LOCUS       DAORAQ010000001        79169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_86_length_79169_cov_69.183318, whole genome shotgun sequence.
ACCESSION   DAORAQ010000001 DAORAQ010000000
VERSION     DAORAQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 79169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 79169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79169
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_79169_cov_69.183318"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1450
                     /gene="hisZ"
                     /locus_tag="PKM72_00005"
     CDS             125..1450
                     /gene="hisZ"
                     /locus_tag="PKM72_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNP59183.1"
                     /translation="MKPLRSLIPIGTATLLPHTAQSIRRLEDQLLAQCSTWGYQEIIL
                     PTFEYLDVLAVGLAPEILEKCYKFADWASGRILVLRPDATAQIARMVAMGLGGETLPL
                     RFSYRTTVFRYEPEHRGRDREVFQVGFEMLGNDTSQSDAEVVTLLAGLLEGIGLPEWK
                     ISLGHVGFFKALLAQSGLSLNGRKQAEIAAAHKDLPQLEKILETERLSRSKVKDILQV
                     PERYGRDEVLEWGMRIAGKDSQLLKPLQRLTQVYRQLIANGVQDHILLDLGEFRGFDY
                     YDGVVFDVFTSTFGSEIGGGGRYNHLVGRFGRNLSAIGLGLDLDRVFSALERGGKVGG
                     NGLQPVRIFTSPHQAEASFALAQRLRGAGICVIEEMIEGSPKSALRWVITVGRRRGLP
                     CALIFEERTSTPQSVLLLEFSSFSQKPRQTRIPVQELPQRLQTFSHDNI"
     gene            1437..2792
                     /gene="dnaB"
                     /locus_tag="PKM72_00010"
     CDS             1437..2792
                     /gene="dnaB"
                     /locus_tag="PKM72_00010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNP59184.1"
                     /translation="MTISEAPEVRVPPQNLEAEQSVLGAILLDNAALNRAMEILSEED
                     FYRTGNRVVYRGMVALSERNQPVDQITLTDYLRGTGELDQIGGASYIAEIVQVVPSAA
                     NIRYHSNIVRDKSLLRGLVRTATDVVMRGYEGTTETNELLEFAEREIFRLAQGHLGGT
                     FAPVSSIIKDSVEIVDRLYSRKERITGVPTGFTDLDNLLAGLQPSDLIIVAGRPSMGK
                     TSLALGMVEHASLHSNAVVGIFSLEMSRAQLVLRMLSSQARLDSHALRTGQLTKHDWV
                     ALTEAAGRLEQAKVFIDDSGNLTVQQMRGKARRLKAEHGLSLLIVDYLQLMEGRGNAE
                     SRQQEISDISRALKGLAKELNIPVIALSQLSRAVENRTDKRPMLADLRESGAIEQDAD
                     VVMFIYRDEVYNPDSDDKGIAEILVRKHRNGPIGEVDLQFHDRYAKFNDLTRVREAGI
                     V"
     gene            2814..4619
                     /locus_tag="PKM72_00015"
     CDS             2814..4619
                     /locus_tag="PKM72_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP59185.1"
                     /translation="MKSTRVLAMKVRAGQTFPMWLGRLRSLLWGIPLLVVSCGTAPSP
                     TLNGQEVSMVSRETSHPQAYYHFLRGSLAELNNDATTAVEEYQAGLAFDTDSNFLKFR
                     LAKLHFSMSHLTEALGLARQIPVSEVSQAAMFFDLAKIFAGGGDTERALEVLTEGERH
                     FPQDERIYISHGTLLLNMKELQKAEEVFHELLVHVPGSAEAHYFLGVIALEAKTKQEA
                     KAHFQEAIALHATFERAYLKLVAISEEEKDSQEGIALLERYLIEVNPHHREFRLRVIR
                     LYIGQQKTDKALNHLDYFLQENPDDLHAQILKAQIYGELGNFPAAIEELNAILRVRPN
                     ELRVRDFLGLLYEEMKDYDRAIQAYRTNVQMDSTFFDSILHLGFVSYRLKRNQEALSY
                     LDQAVNLNPRRPEPYLLLGLTHFQMEDYHKARARLEEGIHQDPSNAELHFNLGTAYDK
                     LDLFDKVVREMEQTLELDPEHADALNYLGYSYADRGINGEQALALTQRAVALKPHNGY
                     YVDSLAWALYKLGRIEEALETIQRAVSLVSDDPVIYEHMGDIFLRQDERDKAREAWLH
                     SLQLDSTNKPLGKRFRDAGFGEPIPTLSHKSTLTP"
     gene            5018..5464
                     /locus_tag="PKM72_00020"
     CDS             5018..5464
                     /locus_tag="PKM72_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNP59186.1"
                     /translation="MLTQLNDQRGFSLTELMIVVAIIGILATIAIPNFLRYQAKAKQT
                     EAKSNLVAIHTAEIAYFAENNGYIDDFNAIGFAMSGSSQRYFYKIGNANLGTLPPGCT
                     DPNLDSVSALGFTAVAIGNIDGDATCDVWTINEGKVITNVTNDVSS"
     gene            5583..6884
                     /locus_tag="PKM72_00025"
     CDS             5583..6884
                     /locus_tag="PKM72_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNP59187.1"
                     /translation="MLDKIKNRSAKVGVIGLGYVGLPLAVLQAKAGYQVFGVDEVAAK
                     VEMVNQGRNYILDVVDSDLREVVEQKKLAATTDFSVLRQCDVILICVPTPLTKNKEPD
                     ISAIVKVLGHLADNSHPNMLVVLESTTYPGTTEEVILPALTATGLTVGKDLFVAFSPE
                     RVDPGNPEFKTHNTFKLVGGVTEACGEVAKTFYEQSIVKIFLLSSPRVAEMSKVFENV
                     FRSVNIALVNELTLLCDRMNINVYEVIDAAATKNFGFMPFYPGPGVGGHCIPLDPYYL
                     AWKSKEYDVHTRFIELAGEINENMPYFVMNKLQRVLNQRGKCLKGSTILILGVTYKAD
                     IEDPRESPATKVMELLQQEGADLQYSDPYTPSLIIQGHEYKSQQINAELLKGSSCALV
                     LTAHSAFDYQLIVDHAPVVFDTRNGTRQVKHHRDRIVLLST"
     gene            7065..9233
                     /gene="shc"
                     /locus_tag="PKM72_00030"
     CDS             7065..9233
                     /gene="shc"
                     /locus_tag="PKM72_00030"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNP59188.1"
                     /translation="MKLLKQFLVRLSGNIFETLTPKLIPYRGKQKPPPLKLVSHNLHP
                     DVSHDPTLQRTANLGHMSALESALQKGEDWLLGMQDPERGFWVQELEADTTLTSEYVM
                     LRRFLDMVDPERERKATRYLLHTQLEDGGWPIFSGGPSEISASVKAYFALKLAGVSSD
                     EPVMQRARDLILGKGGVVQANVFTKITLALFGQYDWRGIPCMPPEIFLAPRWFYFNLY
                     AVSYWSRVVIIPLLIVFAHRPVCAISKEQGIDELFLQPRQEVDYANVPPLHRDSTILS
                     WRNFFVWVDGLLRLYEAYPIKALRKKALKSAQAWVIEHMDGEGGLGAIYPAMAYSIYA
                     LRALGYSTDHPLVQKALREIEDLEVYSNSGNTPTPTMLHLQPCHSPVWDTALIINTLI
                     ERGLALDHSSLRRADAWLRSRQCQKVGDWAVSAPEARSGGWAFQFENEWYPDVDDTAA
                     VITGLAKIHPVELSGSDGAIQRGFRWALALQSSDGGWGAYDKDNNKLIFNKIPFADHQ
                     ALLDPSTADLTGRCLEMLGTLGYDQSHPAVNPAIEFLRKEQEPNGSWYGRWGVNYIYG
                     TWCVLSGLRAIGIDMTVPWIQQAVIWLESVQNPDGGWGETCDSYADPQKAGRGESGAS
                     QTAWALMALLQAGESDSICVVRGVNWLIRHQRENGVWEEPFHTGTGFPRVFYLRYHGY
                     FKYFPIWALSMYRNVKVTGEARADHLRKAAQVARHISKLV"
     gene            9317..10105
                     /locus_tag="PKM72_00035"
     CDS             9317..10105
                     /locus_tag="PKM72_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59189.1"
                     /translation="MTATRVEFNAIGRVLPKGSSVKCVGHAGLESRAESTHVLVIQTG
                     MGPDKARSIAQKILESEVCDVVISTGFAGALNSSPIGSLVIGQDVLLLEQSGDLMSDF
                     VLPPIKCHPDWVNASLGIQLIDGYPLQSGRFVTTDQVLTQASQKGILGKRSGAVAVDM
                     ESGAIGQVAKHHGLPFLIVRSISDGINEDLPVDFNIFLKPFGWVKGVALVLSTPRCWK
                     GLFRLYQNSKQAGIQLSSFFEKFFAIIFTPTFSMAINKSRSEIS"
     gene            10102..10869
                     /locus_tag="PKM72_00040"
     CDS             10102..10869
                     /locus_tag="PKM72_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP59190.1"
                     /translation="MILLQHIGKRTLFLIEEMGAMFVFLIRTFGWLFRPPVRIVQIVK
                     QMYVVGFKSSFVVILTALFTGMVLALQGYYSLRKFGSEGLLGSAVAISMIRELGPVLA
                     ALMVTARAGSAMTAEIGIMRITEQIDALETMAINSLQYLITPKIVAALISVPLLVAIF
                     DVVGIWGGYLVGVKLLGVSGSSYWSSIESAVEWKDVYGGILKSISFGLIISWICCYKG
                     YYTRMSSEGLGKATTESVVLASVLILVWDYFLTSVLM"
     gene            10882..11643
                     /locus_tag="PKM72_00045"
     CDS             10882..11643
                     /locus_tag="PKM72_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP59191.1"
                     /translation="MSLPIIVKLAEVEKAFGEQSVLKGVTLDIPKGKITTIIGPSGEG
                     KSVMLKHIIGLVRPDRGKIIVDGTDLTRINDRELNDIRKKFAMLFQSAALFDSMNVFD
                     NVAFPLREKRRAAEQDICRLVQEMLERVELGNMGHKFPAELSGGMKKRAGLARALVMR
                     PEIILFDEPTTGLDPLMAHAIHKLILAMQKTFGFTGIMVSHQIPEIFPISDWVAMLKN
                     GKIIAMSPSDEFQQTADPVVREFISVNNGIHLAPA"
     gene            11764..12210
                     /gene="mlaD"
                     /locus_tag="PKM72_00050"
     CDS             11764..12210
                     /gene="mlaD"
                     /locus_tag="PKM72_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNP59192.1"
                     /translation="MMERGKLELIVGIFVLVGVICLGYLAIKLGKLELVGGDYYELQA
                     EFSSTSGLKNGASVEIAGVEVGRVKNIGLKQDRAQVVLAIQDGITVFDDAIASIKTRG
                     IIGEKFMELSPGGAGERLETGGTIIDTESGIDLEQVISQFIHGNVE"
     gene            12336..12935
                     /locus_tag="PKM72_00055"
     CDS             12336..12935
                     /locus_tag="PKM72_00055"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNP59193.1"
                     /translation="MAIIFFGWVVVSLACLTVAWGGGTPTGAVKETVDQVFVVLRDQA
                     LKDPGKEMERRAKLEEIIGRRFDYAEMAKRTLAAQWKGLSPEQQQEFVTLFQQFLANS
                     YVGNVDGYSGEEVEYLKEREKGGFAEVQTKVVSPKVQIPLDYRLLQKNGEWRVYDVVI
                     DGVSLMKNYRGQFSRIINSSSFEALLEKLRSKADLGTSS"
     gene            12948..14156
                     /locus_tag="PKM72_00060"
     CDS             12948..14156
                     /locus_tag="PKM72_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNP59194.1"
                     /translation="MISLRFWGLALFRLAVGGAILPSSNALAENSFFVVPAVSSSKND
                     GQDFGLITPILNSDTEGNLRSLVAPMLIHNSFLGVRGTLNYFHYWSGGRQMEAVGSYT
                     EEIERKLKFRFQDPGFIEGLFFVDIGAQFFKNATKRFYGLGQITPQDNETNYTGREIQ
                     VHWNFGIHLNDVTRLAVNQRFRDVEIQPGGVDSEPYAKDRFPGDPGMKGATILAHRLV
                     FQYDSRDNLNTPTAGTRFEAFGELAQNFDAGKNDDMLYFLSGFDIRHLIPSPSKRYIF
                     VARAMVQLSFGSDIPFYEQSSLGGEDNLRGFGRDRFIDKHMVAFNVEERIHLFGMKMF
                     NVGIECELAPFVDMGRTYPNFEFRQFHDWEVTPGVGFRAIVRPNVMARVDWGYSKEGG
                     AVFAGLNYPF"
     gene            14191..14967
                     /locus_tag="PKM72_00065"
     CDS             14191..14967
                     /locus_tag="PKM72_00065"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNP59195.1"
                     /translation="MLTISENMNQYVRLSLFSLMTGLLFLPSGALLGEEVTDQQYPES
                     LEKELGTLMREGAANSTDLTRLLRLAGLYLDLGYGVYIDREQKLAAFQEGARVAQKAL
                     DLRESSAEAHFLYAANLGSAAELEGLVSAALTIQKLKKHVHRVLELDPENVQAHHMLG
                     RMYEELPWVLGGDQELAEDHLKMAVSLDSHYAPAGLDLAKWYLKHDQTLEAVRELTRV
                     VNTPPLKKRWIWERIHRPEAQDLLQQILSQGNVDRSNDHS"
     gene            15094..16989
                     /gene="dxs"
                     /locus_tag="PKM72_00070"
     CDS             15094..16989
                     /gene="dxs"
                     /locus_tag="PKM72_00070"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNP59196.1"
                     /translation="MSLLKRIESPADLKKLSREQFPELCQEIRELIIEVISHVGGHLA
                     SNLGVVELTVALQYLLNTPDDKIVWDTGNQAYTHKLLTGRREQFHTVRQFGGISGFCK
                     REESIYDTFNAGHAGTGVSAAVGFVEAREQLGKSNKVVCVVGDGALTAGMTLEGLQHA
                     GDMGRDCVVILNDNQMSISKNVGAISAYLNRTFTGEFYTRLREETSQLLETIPQIGEP
                     VKRMAIRAEELAKGFLLPGLLFEELGFRYVGPIDGHNFEHLLPTLENVLKLKGPTLLH
                     VITKKGLGFQPAMKNPVWFHACSPFDSKTGQPVKKPGHPSYSSIAAKTLIRQAKKDNR
                     VVVITAAMCEGTGMAEFEKEFPSRLIDVGIAEQHAVTYAAGLAADNMRPVICMYSTFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDFAFLRHVPNMVIMAPKDE
                     NELQHMVQTGLVHDGPVAVRYPRGSSLGVPLDSEPTPLSIGQGELLSDGHDVAIIAIG
                     VMVSEAMKAAERLEEEGVSVAVINARFVKPLDKNLIRDIAKKVKCLITIEEGCRMGGF
                     GSAVLEFLSEEECWSMPTKVLGLPDWYIEQGPQDLLREKYGLTADGIYDQAKALHDRV
                     SLQAAIR"
     gene            16999..18195
                     /gene="ispG"
                     /locus_tag="PKM72_00075"
                     /gene_synonym="gcpE"
     CDS             16999..18195
                     /gene="ispG"
                     /locus_tag="PKM72_00075"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNP59197.1"
                     /translation="MYISRRKTKQIQVGSVKIGGNAPISVQSMTIPHPRDVAGTVEQI
                     QQLEKAGCELIRVAVPDMEAADALPKIKSQTTLPLIADIHFDHRLALKAAKVVDCVRI
                     NPGNIGPWWKTEEVIQAVNDYGIPLRIGVNGGSLEKHLLEKYGYPTAEALAESALNAV
                     HALEDVGFTNMKVSLKASDVHMAMDAYWLFAQQSNYPLHIGITEAGTAMTGAVKSAIG
                     LGWLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGVNVIACPTCGRVEIDVV
                     KMANELEHRLGHITTPITVSVLGCVVNGIGEGKEADIGIAGGQGVGILFKKGKLYKRV
                     PSEELLHTLIEEVELMAKEQGGQEPSINIPETPDVLSGNPSSMEDLSLKSIRSVSREL
                     PVLPRQ"
     gene            18285..18360
                     /locus_tag="PKM72_00080"
     tRNA            18285..18360
                     /locus_tag="PKM72_00080"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18318..18320,aa:Cys,seq:gca)
     gene            18460..19476
                     /gene="hpnH"
                     /locus_tag="PKM72_00085"
     CDS             18460..19476
                     /gene="hpnH"
                     /locus_tag="PKM72_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HNP59198.1"
                     /translation="MSVPVSQMYTVAHYALSQHLRGVKRYPLVMMLEPLFRCNLECAG
                     CGKIQYPDHILNRRLTPEQCLAAADECGAPIVSIPGGEPLIHPEMPAIAEGLVRRKKY
                     VYLCTNAILLERKLQDYTPSKYLTFSIHMDGLKEEHDHAVCRDGVYDVAVKAIKAALA
                     KGFRVTTNTTLFNDAAPARVRRFFDEMMELGVEGMMISPGYSYEKAPDQHSFLSRERT
                     HSLFAQLLAQRKRSWQFNQSPLFLEFLMGKREYECTPWGNPTYNIFGWQRPCYLLQDG
                     YVSTFRELMEETEWERYGSGRHEQCRDCMVHCGYEASAVKDTFSSWGGFFGTVRATLF
                     PNAV"
     gene            19576..19911
                     /locus_tag="PKM72_00090"
     CDS             19576..19911
                     /locus_tag="PKM72_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59199.1"
                     /translation="MSSIDSPQSWEGRCLQPLNEKELHEAMNLVFDYRGDVTIQLKNG
                     EQVVGFVFDRQEEAPQPYIQLFLPGDQHPRMVLYQDVAGIEFSGQDTAFGRSWEDWAQ
                     KWKKSESSS"
     gene            20527..21075
                     /locus_tag="PKM72_00095"
     CDS             20527..21075
                     /locus_tag="PKM72_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNP59200.1"
                     /translation="MNEFQFVKSLLVSGLAVMGLSMSACGGGGEGPPQPPPPAPPEYA
                     DKHMPSGYWNNPEILAEGEAIFTGQQNIDVNCASCHGKDGKPVKAGARDFRRTEQMEL
                     YSDAVWFWRVSEGVSGTKMKAWKSKLSEDAIWKVIAYEASFGLKGKEYDVAKGQWVPS
                     GGAGAAPAAAPPADGEPEKAAE"
     gene            21114..22829
                     /locus_tag="PKM72_00100"
     CDS             21114..22829
                     /locus_tag="PKM72_00100"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNP59201.1"
                     /translation="MKTLNRSLWIFLALGLSGVATAFGQVPDAPLVDFPYSGNRTAVW
                     VVAQLHILFAAFILGAPIFAVVAEWLGYKNNDPKYDRLAKEVIKVTVILYSMTALTGG
                     LFIFVLLGTYPDFSTWLIKHFFLVFAVIYPLLFILETIILYTYFYSWDSMKGAKKGRH
                     IALGILLNIVGTVTLFVIDGPTAFMNTPAKAVEGVSLVEFIQTASLWDKMANFSWMPL
                     NLHRLVGNVTFGGFIAGLIAAYMFMGSKTEEERAYYDWMGFVGNMIGVGALLLLPFMG
                     YLLAYELCDYDASICPYMMADQLSMFFEMQGAMIGLIFLASNYYIWLSLKRIQGVEQV
                     RISGFVAVVVVFMPAIMGFTWKMFPPPEWQSLIVLGILVILPVVLGKVPGLKNFTVSA
                     FTMIKVGFLMIVVADAIWMTPHGFVPTQGLATEELELPSWASELALMPAKNAAAFTLV
                     FLTVVNYLLYNRAIKRGTIIWGKIDFASQFVLIFLAFSVIWTMGLMGTVRSLTRKYYH
                     VYNLVPDFTPEAFTPTLAYSAWWITGVTIVFYAVVSFAILVTLKAGSSKPATSLASSV
                     PAEAK"
     gene            22854..23666
                     /locus_tag="PKM72_00105"
     CDS             22854..23666
                     /locus_tag="PKM72_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP59202.1"
                     /translation="MANQSFAKSVMKKTTLGVIIGVLLVVGAKFTHFPPVFQVMFFLY
                     AMLGALVFILLDAPAMKRLEGVKAVGALVVFYLLLSGLYIAGASILPQFDPEDEKGKI
                     EKVLKLRRAQTEQGKADELIARAKELDERAKAISKQLSSLGADTKVEVVSTPGPAGGG
                     AASGDLVALGKDQWELQECYNCHKLYGQGGKKRGPEMDNIGNLMTPEQLKEKIIDPKS
                     WKAEGFDKDYDKGKMPDKYKDLMFPQEIDALVAFLASLKDESVKTPKPIKMN"
     gene            23692..24636
                     /locus_tag="PKM72_00110"
     CDS             23692..24636
                     /locus_tag="PKM72_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNP59203.1"
                     /translation="MQPKLTAKALCANKEVGKISKVIVDPLSHEISHIIVQELNGHGA
                     QRQIPIGQVQEVVSEEEIVLRCSSEEFGQFPVLERDQYVTIKEVEIAHLEDHLHVEPG
                     EILVPLPRLEQGVPRRTFFTNMTHAIGALIALPLVFPVLKYLMKPMFKPYDNAWFSVG
                     NVKKVNKENIGFQFKFTRGFKEAFMPEQQIEKNIWVVKATPAVQKAVYQGNDMKFYDN
                     KGDVIWVNKSNSPYIGYSGKCPHLGCGYKWRKTKNFPDGVFLCPCHLSIYDEAGKVID
                     GPAPRPLDVLPLQVDAGGEVKIIDVEYKAGVNNQIRLL"
     gene            24665..25777
                     /locus_tag="PKM72_00115"
     CDS             24665..25777
                     /locus_tag="PKM72_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNP59204.1"
                     /translation="MKEDSQIASPQNAFEKVVEFVDERVGLKTIQAKMLNEPIPGGSR
                     WAYAFGSVLLFIFILQVVTGILLMFYYVPSTDHAYASTQYIIHEVDYGWFLLSYHFWG
                     SSAMVVMVFAHMSQVFLWGAYKKPRELVWLVGLALFGIVMGFGFTGYLLPWDQRAYWA
                     TVVGVEIMDKTPLVGDFIARFLKGGPTPGQMTLSRFFVIHVMVLPAALAGLAGLHIFL
                     FRKAGPAGPFRGTPEEIKAKTDYFFPRQIWKDIVAMATVFLIICSLAFIEPVVLLEKA
                     TPDPGDYHPEPEWYFLFLFQLLRLKIFGGEFGQFLGAMAIPGAFMAFLAALPFLDTNS
                     ERNIFKRPIALVGWTVIMVFILVFTVSAIINRHFLD"
     gene            25791..26687
                     /locus_tag="PKM72_00120"
     CDS             25791..26687
                     /locus_tag="PKM72_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59205.1"
                     /translation="MSNTKLGLLVFWPTFWTGFPIKMVVALLLLAAHIHPWEGSGLFL
                     LLLVSIPVDIWALGLCARTVFIDRLKVDPRPGIGLQLWIRWAVFSALALPILLFVVST
                     VAETAQSVVSSIVESIKENIFVIPVAEQISLELVMWGSVASVALILCLFGWLFGLGWL
                     AQPFVKDAKPVVGSAVDQANFWDSLRIPADQPLLLTAFTGLGVVLVFLFWVLLPPTTP
                     HPHEEYVYTFEKKEKEIVEPKKILMEAEQVLANAELAVLKLQEENPLDSESAVKSKDL
                     PKESKQNNKIDTQVPVKGEGKP"
     gene            26808..28616
                     /locus_tag="PKM72_00125"
     CDS             26808..28616
                     /locus_tag="PKM72_00125"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNP59206.1"
                     /translation="MPGMLKLASFLALLFLPALGMAAGGAEIQAMSVEYRDVPGIGSR
                     NLIWIVAQQHLLLAGFVLGVPIFAWICELVGWKTKEARYDKLAKEFTKLLTSAYATTA
                     LFGGILLFLLIGLYPKLMAYLTDMFFPSFLVYCLLFLLETATLYMYWYGWDYMQGNKK
                     TFHLFLGFLLNLFALGIMAVPNSWATFQASPVVLGEGDAWSRAWMAMQNPTWMPVNIH
                     RLIANVVLGGFIVGAYAGIRYLLAVSREEREHYDWMGYVGNFIGVFGMLPLPFAGYWL
                     MREVYQYNQQMGITLMGGFLAWLFILQAMLIGVLFLGANYYFWMGITYRIPGSENQYK
                     KPVMAMLIVLLLCLGIWMTPHSLVASLAEAQKMGGTHHPLLGVFGVMSAKMTVSNIMI
                     LVTFMSFIMYWRAGKQETAGWAKAAKAIMGGLLVIAGIAVVVLGVWGYFVPAIIRINY
                     FSVAQVLIVLFIMITFTPLTAILMKSAKTTTEMVWGKMPIRAGYSLVLNAVMVILLMS
                     LMGYARSSSRVHWHIYGVMRDTSEYAYSPALGYAAAFMSLNTFVFCLIVAFIFWVATL
                     GDKAKAQPPRGSTVDIPGHSAPAMAGGAPRAVEKLE"
     gene            28677..29315
                     /locus_tag="PKM72_00130"
     CDS             28677..29315
                     /locus_tag="PKM72_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP59207.1"
                     /translation="MSEVALLQIIGMCVIGVGILILLFIKGMFLRVLGFVAMVLGVFS
                     LIALSVPQMASLPPAVETFDLASVKSPDDLASIGQKIFFSKGQCALCHSIGPSESARC
                     PDLNGIGAKLSPEFIYESLTQPQAYIYLDFRHDGVPKEYPAQMPHIDKDPIGLSKQEI
                     YSVIAFLQKMSGEPISIKVEDIMETEQKSDDSLKVASVSSPLKSQMENSADR"
     gene            29344..30069
                     /locus_tag="PKM72_00135"
     CDS             29344..30069
                     /locus_tag="PKM72_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP59208.1"
                     /translation="MKGPITSGAIVLAGVFIFLKFLLPLFTAPLPASLIYLYLALALS
                     GIMIYGTMSGPSLEAMMGPVFRFLSGQGQSGIGHMARLAVLVMFPLLVGWQTYSSLAP
                     SDLPPAENRTIHPAPPGEFVGLSNPYPKTPENIMMGKGLYAAYCSPCHGANFDGKGTA
                     APGFNPPPADFTDPGTIAQLQEAYLFWRIKKGGVGLPVEGMPWKSAMPRWEEELPEEF
                     IWKIIMGEYDGAHQSPRTWEEEE"
     gene            30114..30950
                     /locus_tag="PKM72_00140"
     CDS             30114..30950
                     /locus_tag="PKM72_00140"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNP59209.1"
                     /translation="MSNGLRSLGAISIIGLFGVLGFTETFASEPPASDELGSVSVRMY
                     QLEGSLPTADPNSSQWDSVPGSEFNLAPQVHWPPRIQEVTVKSVKAKGVHNGQELAIL
                     VEYDDPTEDPGDAAALEFMVGDKPAHFAHGQEMLQVEGGPVNIWFWKKESGQAVDMSA
                     KGFKTLETQPHQDVQATGVWQNGTWKVVFTRLLKTEDEQDVQIEPGEWRNVAFAFWDG
                     AIVDGLVKEKGGQKAVSTWWYIRAEPSADNSIFGWVILGLALAAAFELVIVRKLRKGQ
                     SV"
     gene            30947..31984
                     /locus_tag="PKM72_00145"
     CDS             30947..31984
                     /locus_tag="PKM72_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNP59210.1"
                     /translation="MKLIAGGGRDITVIGLAVVGFTFTLFSGCAIFEDEKTSKGRKLY
                     NHYCSHCHGETGQQGEGFNWERMSDPRPKNLASNAEMSTFSDEEIFHTIYRDMKDTTD
                     PKVLDDEDYFAVPTMPTFKYTLSEEEIWSIVGYVRTLHGMSLSYDFEARKKELDEAFQ
                     AAQQEMETAKASFEAATEKAEAAQAAYEEALTDEQLENYEPEEVTIPEEEAFLAANEK
                     YENAKASVDNFLKRPKKAQVSRPDLSLSEEERAVQANLGKQLYNDKYGCQGCHSLNDE
                     GGLVGPALDRAGFRLNPTWVYRWILYPQAMKKHTRMPNLGISDQDAKAITYYLGTLQA
                     PKPENPVPLSE"
     gene            complement(32235..33086)
                     /gene="ubiA"
                     /locus_tag="PKM72_00150"
     CDS             complement(32235..33086)
                     /gene="ubiA"
                     /locus_tag="PKM72_00150"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNP59211.1"
                     /translation="MPPFLAFANLIRLFNQTGTLLLLFPTLWSLFLASEGWPGLKLLL
                     VFIFGSFLMRSAGVIMNDLADRSFDREVQRTRHRPLASGQLTPSQGLALLAVLLGLAA
                     ILLWLLNPLTLLLSPVALLLAGIYPFCKRFIHIPQLVLGLAFGWGGIMAWAAVRNELD
                     LSAWILFAATVCWAMAYDTIYAIQDRDDDQRIGVKSTAILFGSYTWLGVGIAGLVMLA
                     CLSLVANINHLGIEYSMTLMGVAGLLGYQVLLLRSNISGEKAFFLFKQHVWIGVLILG
                     GIWMGHP"
     gene            complement(33233..33694)
                     /locus_tag="PKM72_00155"
     CDS             complement(33233..33694)
                     /locus_tag="PKM72_00155"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNP59212.1"
                     /translation="MKHILRGIGLLIGLAGTPLLAQADFLVKTVVLSKEVDKRNPQGI
                     FRPPAYCEKDKNGQAAIPMIQTSQTSQVIFWTKIEATTKGKIRHSWHHKTDGTWTKIS
                     EVNIPIQPSPGYRMWSMKSLHPDLHTGEWMIVVAPSNEPDRILCIARFMVK"
     gene            33973..34047
                     /locus_tag="PKM72_00160"
     tRNA            33973..34047
                     /locus_tag="PKM72_00160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34005..34007,aa:Thr,seq:cgt)
     gene            complement(35992..39453)
                     /locus_tag="PKM72_00165"
     CDS             complement(35992..39453)
                     /locus_tag="PKM72_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HNP59213.1"
                     /translation="MTEAYSASPPPATQSSALRGLLVAQFFGAFNDNAWKLIVALLAI
                     KQVAASAGGASGPELEAATQATTTQAFVIFTLPLMVVSAFAGVFSDRFSKRTVIVVMK
                     AVEVLLMGAGTAALFWNPLGGILPLLVLAAMGAQSALFSPSKYGIIPELLPHHRLSWG
                     NGQIEMWTFIAIIAGTALAGLMLDLSGQTPWLTGLVLMMCSGIGLWASLFIPTVPRAR
                     LDGGVRETWKAAIEALRFDRVLKLGIMGAIAFWTLASLVGQDVLIYAKVVLHLSDSLS
                     GLPLAAFGIGVGIGSLLVGKLSAAKVELGYLPLGGTGITASLFALGFGEPQLGGTLIA
                     MGCLGLASGFVVVPLNALIQWRSPADRRGAVIAFANTLVFGGVLLGSLGSGFFSKIGL
                     SASNIFLISGLGSAALTIWALRVLPEMFIRLLLVLFTHTIYRLTITGRDHIPQEGGAL
                     LVPNHVSFIDGLLLLSTTDRPIRFLVDQNYYDHRVFQPFAKIMGVIPISSNGSPREIL
                     HAIRQAGQSLDQGELVCIFPEGQITRTGNLLPFRSGFTRIVKGRDVPIIPINLDRVWG
                     SIFSFIGGRFLAKWPTRFPYPITLSVGAPLPSTTSAEEVRQAVQELGETAWHLRKLTR
                     RPLHHSFVWSMRKHPFRVVFGDATRPRLSCFEALTGAIALARALRSHWEGQQTVGILL
                     PPSVGGALANMAATLSGRTTVNLNYTVGVQGMESSSNQAGLVTVLTSRVFLEKANLEL
                     PMNLTPIWIEEIRNTIDGQARITAMLFGLFAPIRKVERFCGATMHPSIDDIATIIFSS
                     GSTGEPKGVPLSHFNLDSNVEGIAQVLHLDHNDRILGILPFFHSFGYLTTLWFPVIHG
                     AGVIYHPSPMDAGPIGNLIHKHRVTILLTTPTFLQLYLRRCTPEQFGSLRIVLTGAEK
                     LPDRLLIAFEERFGIRPIEGYGVTECAPVIAVNCPDFRASGFFQSASRRGTVGQPLPG
                     VSVRIVDPDTEQPLPVGTPGMLLVKGPNVMSGYLGREDLTAKVIRQGWYITGDIAALD
                     EDGFITITDRLSRFSKIGGEMIPHGRVESALMEAANTETMTLAVTAVPDERKGEQLVV
                     LHTLDESAIPEILEKVTTHGLPNLFIPKRSNFIKVDRLPVLGTGKLDLRTLKQLALAK
                     IKAQLPQ"
     gene            40096..40956
                     /locus_tag="PKM72_00170"
     CDS             40096..40956
                     /locus_tag="PKM72_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59214.1"
                     /translation="MTTRTETSGFSEGHMEAVGESIFHRMRNVLNSIHVSAGLIHHRL
                     RESHVADVGRVADMLEAHSHDYGWYLTQDPKGKKIPNFLGQLNEELSQNHLDTLTELT
                     TLNYHLEQLEYYLTVGQTPHRVGGFKDTIQFAAIMDEALASHQCELDRMGVQVCREYQ
                     RVGEGILEVSKLLRILVNLIRNAINGMREITSWTHRLTLYVFPCPDREGFVRLQVADT
                     GRGIPLEGLTRVFSPQASGEQTDLLPNLHASALAAKELTGSLRVWSDGLMQGAIFTLD
                     LPVIHMEGER"
     gene            40956..41519
                     /locus_tag="PKM72_00175"
     CDS             40956..41519
                     /locus_tag="PKM72_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59215.1"
                     /translation="MEKEQANRRILVIDDNVAIHEDFRKILERSLDTSLLQEVRAELF
                     DDVEEQKIVEKFEVETADQGQMGHRMVQDAMKAERPYAVAFVDMRMPPGWDGVETVEH
                     LWQEDPDLQVVICTAFSDHAWEDVIQRLNKNGQLLILRKPFDNIEVWQLANSLTKRWA
                     ESCQAKSQLELLAKWAEERAEETIKSF"
     gene            41787..43073
                     /locus_tag="PKM72_00180"
     CDS             41787..43073
                     /locus_tag="PKM72_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNP59216.1"
                     /translation="MSESNGQVIPKFPLLRVVMVGIQESDLSPSFHRVLASQGPILQV
                     TWLDYASMHPSCFDSHNPFDLILVDCRVNRRGRDIACDIKKIWGNAFILGLIDGESSA
                     VELEDDSAIDWLVNEKMEGIAMPWAIREGLVRRRGLIEREHLRRQVEGASYGNEMADV
                     ASTVLHNVGNVLNSINVAVHVVHELMNQSSIILVHRIAELLKNHEENWEIYLTQDPKG
                     KRIPPAIMKLGSHLIAEQQTVLKELEGLVRNIDHVKQIIISHQSMAKSRGIIEALSVV
                     ELLNQAVELSFHAGDAKWIRIKRDYQSVPPVVTDRHQLLQILVNLLRNAKQAMQLQVG
                     VDHLLTIRVEGRTDDGLSVVIAIQDTGIGIAPEHLARMFTRGFTTKHDGNGIGLHSSM
                     VTIHRMGGMLKAHSDGIGTGATLTLILPVEAGAGQA"
     gene            43070..44530
                     /locus_tag="PKM72_00185"
     CDS             43070..44530
                     /locus_tag="PKM72_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59217.1"
                     /translation="MNPVCTLNRRILVIDDSPDILQDFRKILCSAISLISNAWLESSQ
                     DFFDGPFLHHGEEPFDLHCVNNGESGRDVVAQAKERGSPFAVVFLDVQLPNGWDGIET
                     VERIWDEDSDVQVVLCTAYPDFGWSDVLPRLGHRDQLLILRKPFDPIEVWQLSTALTM
                     KWHCAQQARLRVQELEQTVMTRTGLLEETNRCLEQDLLRRQEVEVELAQVVCDVQERN
                     LELSAMRDQALNEIHERERIERILRHQTEELARSNRDLEQFASLAAHDLQEPLHSIQV
                     FLDLLQVKYGSALTSQGLGYVDRVKNAADRMQQLIQSLLVYSRIDLPHTSGEPLVLRE
                     MVDEILSDLGARIEEMQAVVQIGELPTIYGNAFQVRQLLQNLLGNALKFHRPGVPPVI
                     RITGTIIQNRRHTGLGQPGWLCQIEIHDQGIGIPSEQFGKIFGMFKRLHRKDEFEGTG
                     IGLAVCQRIVEKCGGAISVRSKLGEGSTFIVTLPIQ"
     gene            44625..46478
                     /locus_tag="PKM72_00190"
     CDS             44625..46478
                     /locus_tag="PKM72_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP59218.1"
                     /translation="MVQIPAHRRVLVVGAHPETQIMLRDALSAPAVGDMSVGSSLNGK
                     NLATSGIQPFLTQTQFALVSVEMGLHVLSKFAEAQVTGNPFVLVLIDGRSEDWEVVRD
                     LVESLWEYDQTLRCIFCLPPDRASWPHRLVVSRPEQWAVLRSPFLEEEVFQLASCLSL
                     PYPVSGETVLNGATYSGENGCHSKSPSDLHVHNGEAATGALESARGELAASRYYVDNI
                     LRSMADSLLVINADMTIGAVNPSLLNLLGYQEDELIGESPGLIFGEEFSQGAIIENLL
                     LQGSVSGVESSFLTHEGQKITISVSGSMMQDLQGQFQGLVCVAQDITERKRMEEEKLQ
                     LNERLMETSRQLGMAEVATSVLHNVGNVLNSINVSIGVITDLLKNSMVEDVGRISQLL
                     DKHHEDLGTYLSQNPKGKQVPGYLEKLSGQLKEEKRVALLELDRLREYAGHAQQCVAT
                     QQDLAKPSGMTEPVCVAEVVAEALAVNQEMLEETKITVIQEFQEVPQLIVDKHQLLQI
                     LVDVIRNACQAMESVARRDLVVRVKLVIGPPDSICLEVQDTGSGIPPDDLTKVFGQGY
                     STKYGGRGLSLHHGALMAKNMCGALRAHSEGLGQGATFFLDLPGNFHFPDL"
     gene            46825..47541
                     /locus_tag="PKM72_00195"
     CDS             46825..47541
                     /locus_tag="PKM72_00195"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP59219.1"
                     /translation="MLIRGHTSFDELQAQKLSHFDREVKVHVEAVSGDAGMIPVDLER
                     ALEFGVEVFLLSEGCAVESSRIVSLWRHSLRTGYLAALIALNQGSNQRMVWQSFVGGV
                     LHDIGMLVFLTQQPEMFTTVVEIAQCRGGNLAVLERQVLGYTHGESGVTFLARWGIED
                     MLLAIVAFHDDPSKVPHTTFCPLTAVYAANFVEGGGIAQDGDGVIGKDGEEYLTRLGL
                     WDDLPIWQSWMPNIQQMAIH"
     gene            47901..50153
                     /locus_tag="PKM72_00200"
     CDS             47901..50153
                     /locus_tag="PKM72_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNP59220.1"
                     /translation="MRILSRHLIGGVIVGACILFLPQTVVSLDPPSAPCQNSEGCVTK
                     FLEQLESFGADSEAGWDHAKSFNQIVTSNDNSALVKRVGFRYGYWLKESSPLEAIPLL
                     KSALHDFPILGDYLTFWMGQAYAYAGLGREAAEAFQQFNEHYADSLLRAEALYAGGDV
                     LAKLGDCDAALSMWSQALSIKSDHPKSAEAFFQMGLCSAQIGQREKAIEIFREIWWKF
                     PLAPERLKAESWLRREVGSLFLPSPEERYKRSMALYNGGALEEAVQEFQTITTLSSPI
                     PQFFQVQYTLAMALVRLKRYDQAEAILTSLSRSSSSRTDDAWVWLGRVYLRQGKGAEL
                     EALVETSAMGRVTGDQQSLLFTFYGIWLEDHARWLEAGKAYRRAGAVARTLTQRLDAL
                     WRVGWIHYQQKQFIDAIEIFQEITQAVGTPQTESSLHAASQASYWLARAQEHLGQMEA
                     ARERLKKLSQDYPFTYYGQLADIRLGPSEFSTKQWAVLASTDVMPVDTPTHLQQDIHY
                     QKIQELRTLRLHKEAVNELEVVSARFGADPKAFSQLVSLASEVGAYDVGIRLSIRHFG
                     STLRKGQLHASSAAWLGAFPMGYQRVIQSFAPKQVDPFLVAGLIREESLYSARVVSPV
                     GAIGLMQLMPETAKRVGRQLGLPASDSDRKGLVEPNSNIQLGTYYLGQLLNEFQGNII
                     YSVAAYNAGPQAVKRWIGQNGHRDPDEFIELIGYRETRGYVKRVLGSYRIYRTLFGDV
                     CRGVSLDRFC"
     gene            50203..50436
                     /gene="zapB"
                     /locus_tag="PKM72_00205"
     CDS             50203..50436
                     /gene="zapB"
                     /locus_tag="PKM72_00205"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HNP59221.1"
                     /translation="MSVDKLETLEVRVKKLLDLVIELRQDKSHLEQELESTRERLAKH
                     EEMSEGWEEERTTIRSRIEKVLGELEFLDRSND"
     gene            50453..50746
                     /locus_tag="PKM72_00210"
     CDS             50453..50746
                     /locus_tag="PKM72_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNP59222.1"
                     /translation="MTRTIEVEIFGHRISLQGEGDEAYFHELAGYVDAQMRNLAQKTR
                     TSTPTKLAILAAINITDQLFRQERHRQFGEAEIERRTQVLLETIEKHLETNPN"
     gene            51450..53012
                     /gene="rny"
                     /locus_tag="PKM72_00215"
     CDS             51450..53012
                     /gene="rny"
                     /locus_tag="PKM72_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNP59223.1"
                     /translation="MDTVIPILALVIGVLVGFVLSKVYQQQRDLKRRSAAEEQVQQLT
                     QNAQREAENLVKEAKLEAKDLLFQAKAELEAKEKEKRNEIQVADRKIIQREEALERKV
                     SQFEKRDEEMRRKERSLKEKEEVLDNKAVACDQAIKEHRLALEQVAGITAEEAKRHLL
                     GEIESEARMDSALLTKRILDEAKETAEREAREIVTRSIQRITRDYVNEATISVVPLAN
                     DSMKGRIIGREGRNIRALEAATGVDLIIDETPEAVIVSGFDPLRREVAKIALERLMQD
                     GRIHPTRIEEVVEKVKGDLDKLMRDDAEKVIFEVGLSDFHPEIVKLLGRLKYRTSYGQ
                     NNLYHAREASYICGIMASELGLDVKLAKRGALLHDIGKVVSHEEEGTHAMLGAELAKK
                     YGESELIVNAIAAHHEQVEPLCPESVLVAAAEALSAARPGARRETLEAYVKRLEKLES
                     LATGFTGVDKAYAIQAGREIRVIIRQGELNDSESFALSRDLAKKIEQELTYPGQIKVT
                     VIRENRFIEFAK"
     gene            53033..53812
                     /locus_tag="PKM72_00220"
     CDS             53033..53812
                     /locus_tag="PKM72_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNP59224.1"
                     /translation="MNILFIGDIMGEPGRRVILKHLSRVIQEHHIDLVIGNGENAAGG
                     FGITQEVAEDLFDLGLSVITLGNHAWDKREALEYLQKEQRVIRPANYPDGVPGKGTCI
                     VETVHGERLGILQLMGRVFMPMVDCPFRVAERELAQLTTQTNCILVDMHAETTSEKMA
                     MGYFLDGKVSAVLGTHTHVQTADEQILPQGTGYLTDVGMTGPVQSVIGMKPNLVIQKF
                     LTQLPRRFEVATGPSVLSAAVLNLNHSTGKTTHITRLRVFE"
     gene            53823..55166
                     /gene="xseA"
                     /locus_tag="PKM72_00225"
     CDS             53823..55166
                     /gene="xseA"
                     /locus_tag="PKM72_00225"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNP59225.1"
                     /translation="MSGLFPSVLTVSTLTRQIRSTFEKNFPPLWVEGEISNLRCPSSG
                     HRYFTLKDQSSQIRAVLFRSQVERLKFALQDGLEVFVFGRLSVYEPRGDYQLLLEAVE
                     PKGIGALQLAFVQLKAKLEAEGFFETIGKKVLPLYPQRIGLVTSPSGAAIHDLLTIIH
                     RRWPLAQILIAPVSVQGDEAAGQIAKAIGTLNQIGELDVLIVGRGGGSLEDLWPFNEE
                     IVVRAIASSRIPVVSAVGHESDVTLADLAADYRAPTPSAAAELVVPDCSTVRRYLGHQ
                     RVGVERSMRSFCVAWRGQIQELTGRLPEPRLVLGQFVQQVDELERQLYVKMESWCRSL
                     HVRLLKHQSTIWERNPLIDIRRQQLALIDRGIRLARGMSGCILLKRHQSELWMSQLNQ
                     LSPLAVLARGYSIVHNQQSGKVVKQSTEVSVGESVRTRLHEGELICLVERIHPST"
     gene            55278..55523
                     /gene="xseB"
                     /locus_tag="PKM72_00230"
     CDS             55278..55523
                     /gene="xseB"
                     /locus_tag="PKM72_00230"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNP59226.1"
                     /translation="MAVLKFEKALSRLETIVAELERGELSLDESLRIFEEGVKLSKTC
                     LKMLDDAERKVEILVQDKDGRKRIQAFSVEDSNLPES"
     gene            55590..56414
                     /locus_tag="PKM72_00235"
     CDS             55590..56414
                     /locus_tag="PKM72_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNP59227.1"
                     /translation="MKVVQPSAPSRKSSKERLDSLVLNHELVSSREQACRLILAGRVK
                     VDGVLIDKPGKLVATTVSVEVTKPEYAYASRAGEKLAPALDAFCISCLDFVVMDVGAS
                     TGGFTDCVLQRGANRVYAIDVGYGQLDWRLRTDHRVVVMDRCNIRHLCPKDIPEPVDL
                     AVIDVSFISLRLVLPVILPVLRDQAYLVALVKPQFEVGKGQVGKGGIVRDERLREQVR
                     DGFVDYARSLCLDVIGVMDSTLLGKKGNKEMLVGMKKNGKMSAEGSKPIACGSPLP"
     gene            56439..57368
                     /locus_tag="PKM72_00240"
     CDS             56439..57368
                     /locus_tag="PKM72_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNP59228.1"
                     /translation="MSTKILVTGGAGFIGSHLVDRLIQEGNEVIVVDNLSTGKRKNVN
                     KKAQFYKMDIQSKRIERVFRNERPLIVVHLAAQMSVRHSTEDPSFDAQVNILGTINVL
                     EHAVKQGVRKVTFASSGGVVYGEQEVFPAAESHRTEPLSPYGISKLAGEKYLAYYTNA
                     TGLRYVALRFANVYGPRQDSEGEAGVVAIFSKQMLDGGQPIVNGTGKQTRDFVYVDDV
                     VEAILVTLGEDVQGIFNVGTGQETTINECYGIIKNLTKCQCKDLYGAAKKGEQFRSVL
                     DVTKLREVFGWDPQVTLEEGLTRTVDYFRGTEK"
     gene            complement(57419..57739)
                     /locus_tag="PKM72_00245"
     CDS             complement(57419..57739)
                     /locus_tag="PKM72_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNP59229.1"
                     /translation="MSYTITVRDLKARMDKGDQIFLLDVREPHEYSIAKIEGSVLIPL
                     GQVPHSLKQLDPSAEIVAYCHKGMRSADAVGFLLQQGFSNVKNLIGGIEAWSIEIDQS
                     VPRY"
     gene            57850..59151
                     /gene="asnS"
                     /locus_tag="PKM72_00250"
     CDS             57850..59151
                     /gene="asnS"
                     /locus_tag="PKM72_00250"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNP59230.1"
                     /translation="MSELPVVSIEHIAKFEGQDITIKGWIRNRRSSGKLSFLTLRDGT
                     GDLQAILSKAEVGEDQFILSGQLTQESSVVLTGKPRKDVRAPGGFELDVSKIQVIQIA
                     EPFPIQPKEHGTGFLMEYRHLWLRSRRQHAILRVRHEIIRTCRNFFDDRGFTLIDTPI
                     FTPNACEGTTTLFQTQYFDQVAYLAQSGQLYGEAAAAAFGKIYCFGPTFRAEKSKTRR
                     HLMEFWMVEPEMAYADLPDAMQLAEELVSTVIQRVVDTRRPELEILERDIPKLEAMVR
                     PFPRITYEEALTRLQQKGVAIQFGDDFGADDETSLAQEFDRPVIVHRYPAAAKAFYMA
                     KDPERPDLALCMDMLAPEGYGEIIGGGQRIHDLDELLQQIQAHDLPQEAFKWYIDLRR
                     YGTVPHAGFGMGIERVVAWICGLDHVRETIPFPRMLYTLYP"
     gene            59344..60258
                     /gene="rsmH"
                     /locus_tag="PKM72_00255"
     CDS             59344..60258
                     /gene="rsmH"
                     /locus_tag="PKM72_00255"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNP59231.1"
                     /translation="MNDVHIPVLVEEISFWLNPLPGGVYVDCTVGLGGTSLKLLEKSG
                     KNAHIIGLDRDYQAVAIAKKTLSEYESSVNIEHGNFSHIKDVVQKSGYEKVDGVVFDL
                     GVSSMQLDQPERGFSFSQSGPLDMRMDQTQKMTAADLVNHLPEKELADVIFTYGEERY
                     SRRIARAIVQARNVSLIQTTQALVAVLEGALPYAYKKGRLHFATRTFQALRIRVNREL
                     DLIEPALRDAVDLLKEGGRVCVVAFHSLEDRIVKQTFRSMAKREHPSVALLVKKPVIP
                     SVLEIQQNPRARSAKLRVAERLPEGVTL"
     gene            60255..60602
                     /locus_tag="PKM72_00260"
     CDS             60255..60602
                     /locus_tag="PKM72_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59232.1"
                     /translation="MRKTVFLGVMILLGVWCLLYVGVKIDMWRLGYAIEELESQRAEF
                     KKQQEGLQVRLSQLTDPQRVAQRAQSQLGLTVAQEGQIVMVSLDTLPPSESGENFPVR
                     LVREFSDTIVSLP"
     gene            60599..62368
                     /locus_tag="PKM72_00265"
     CDS             60599..62368
                     /locus_tag="PKM72_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNP59233.1"
                     /translation="MKNPSPDIHPVCRIRSGIVCFGLLCGFALIGCRLFYLQVLQADV
                     GAHQAQQQHEKTVVVQPDRGVIVDSQGHPLALNVEVASLYVRPPSLNDPQRVARSLAP
                     ILQMPAKELRDLFTRNQPYVWVKRNIPESVVKELEALNISGLGLTKEPHRFYPKGELV
                     SHLVGFAGIDSQGLEGVELQYESYLRGEKNLVKYQRDALGRKLASPPSHDSVPLSTGY
                     HLTLTIDEVVQFIAEAELAQALKQSGAKSGSIVIMDPLTGAILAWALHPTFDPNHLGQ
                     FSTQDWRNRVVTDSYEPGSTLKIVVAAAALEEGVMSPDTLIYGGDGKMSVAGTIVHDP
                     AKTSWMTFSEALAQSSNVGAIKVAMELGQGRVFRYLKAFGFGEKTEIDLPGESSGILR
                     EPNKWSGRSLSSIAMGQEIAVTPIQMVTAMSVVANGGWLMTPYVVSSILDSHGQAVLT
                     KDPQPKRRPISLETTTTLTRILEAAVEVGTGKRARLAGYRAAGKTGTAQKIDPKTGSY
                     SSSQVIASFVGFAPVERPRLAMLVVIDEPTIGNWGGEIAAPVFRKVAERVLPHLGVLP
                     GGSAVIRTAAANSPISSPQPIVQ"
     gene            62372..63883
                     /locus_tag="PKM72_00270"
     CDS             62372..63883
                     /locus_tag="PKM72_00270"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNP59234.1"
                     /translation="MTLRELLASLDMVLSEGDLQPEVLSITEDSRQVKPGSVFVAIKG
                     EHYDGHQFVRQAQQQGAVGVVVEEDFERHAPLVGGATALIKVKNSRKALGLLAAKLSG
                     LPSAHLHMVGVTGTNGKTTVTYLAKSLLEAQGHRVGLLGTVGYVFGTEHRAASHTTPA
                     SVELQDMLKAMVNAGSDAAVMEVSSHALALDRIAGCEFDIVVFTNLTQDHLDFHHTLD
                     DYFQAKLRLFTECVDSGQKTRPKRALVNADDERGPLILQHCSIPTWTYGLHAHADIRA
                     EAIRLSMGGTDFQVNSPHGLLTIKSQLVGEHNVSNMLAAIGIALEMGMTVPMIEQAVQ
                     SVANVPGRFERINEGQPFTVVVDYAHTEDALYRLLRAAKAITEGRIITVFGCGGDRDA
                     GKRPKMGQVAARHSDVVIVTSDNPRTENPHTILTQIEQGIKALRPEERCSYQIIADRA
                     EAIHAAVSEAKGGDLLLIAGKGHEDYQILGTQKIHFDDREEARKAIQRQMSSR"
     gene            63899..65314
                     /gene="murF"
                     /locus_tag="PKM72_00275"
     CDS             63899..65314
                     /gene="murF"
                     /locus_tag="PKM72_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNP59235.1"
                     /translation="MAAFEIDEVLDATEGKLILQGAQKRKMLRVQTDSREIKSGDLFL
                     ALKGEKFDGHDFVKTACKLGARGVIVEEARAGEMLTQVRRAGLGPVMVVEVKNTLRAY
                     QGLARYHRLRFSIPVVAITGSNGKTTTKEMVYQVLATRWRVHKTQENFNNAIGVPRTL
                     LGLHAGHQAAVIEMGVDQVGQTTQLCDMTRPTAGVITNVGPDHLEFYGTMARSAISKA
                     ELLDWLPQSDGGAILNADDRYFEKFSRKAKCPVISFGFSAQADVRAADSEWNGRRTAF
                     RLFLPHRKLAKRASVRIMGPHNIANALAGAAVGCAMGLSGDDIVSGLHKFRPAPMRSE
                     IRRWGGVLYLYDCYNANPASVKVALESLVGLNSTRRTIAVLGDMLELGPKEAQFHQEI
                     GREAAKLHVTHLIACGAFGHNMSEGVQNVHGPTQVSVVRDAMEAGALLKTMVKRGDAV
                     LIKASRGAKMERVLDAVRPGS"
     gene            65445..66521
                     /gene="mraY"
                     /locus_tag="PKM72_00280"
     CDS             65445..66521
                     /gene="mraY"
                     /locus_tag="PKM72_00280"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNP59236.1"
                     /translation="MLYLWLYPLHTEFAIFNVFRYLSFRIIYAAVTAFLIVFFLAPPM
                     IKKLQTLKLGQKIRTDGPQSHLGKSGTPTMGGLLIIFSVLLSTVLWADITNFYVWLVL
                     LSLVGFGLIGFVDDYIKILRGQSKGLTATQKIVGQVGVALGIGMFFYLSPGYSTELSV
                     PFFKSFTPDLGVLYIPFAILVIVGCSNAVNLTDGLDGLAVGPVIISSMAYTVVAWAVG
                     NKLVSGYLLVPHIEGAGELAILTASIVGAGLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVVCKHELLLILVGGVFVMEAVSVIFQVASFKSRGKRIFLMAPLHHHFELKGWEEP
                     KVVVRLWIIAIILGLLSISTLKLR"
     gene            66553..67959
                     /gene="murD"
                     /locus_tag="PKM72_00285"
     CDS             66553..67959
                     /gene="murD"
                     /locus_tag="PKM72_00285"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNP59237.1"
                     /translation="MEAVKVPPVFPIVNWPNKRVTVFGLGRSGRAAADLLLDVGARVT
                     IVEEHTIQDFESTSAAYGLRGAQVFRGDEVADGLRDLELLVVSPGVPKDHRLLNEIGQ
                     RGIPIIGEMELAGWFLRAPIIAVTGTNGKSTTVRLIGSILQQSGKRAFVGGNLGIPLC
                     EAVPKRTNPSSTPDYEYIVAEVSSFQLETIHRFRPWIAAVLNVTPDHLDRHPTQEDYQ
                     AAKERIFENQTIQDWALINADDPVVRTMALSARSGICEFSLTKKVEQGVYLEGGDIKA
                     RMHGEDLLLASRDALPMRGDHNVANAMAAMGIGLLCGCSAEDMVRALQTTPTFEHALE
                     VVREWQGITFVNDSKGTNVDATLKALQSFQEPLILILGGKDKGGDFSQLVDRMTRQVK
                     GVVIMGEATQKILKAFDQIGPPVALATSLTDAVSQAVAFASRGDVVLFSPACASFDMF
                     RNYHHRGLEFKRVVGELQ"
     gene            67978..69138
                     /gene="ftsW"
                     /locus_tag="PKM72_00290"
     CDS             67978..69138
                     /gene="ftsW"
                     /locus_tag="PKM72_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNP59238.1"
                     /translation="MRRKNSHVDSRAWADLFPRNLGVGSKRIDPLIVGITLALSLGGL
                     VMVFSASGVMAENKFTNATYYLQRQIVWMVLGFSVLLIGSLIDYRQWKQWIPMVVGGC
                     VVGLLLVLAVGPQINGARRWLALGFFSIQPTEMAKLAVVLYLAAFLSNPQRRVTDWQR
                     GFLPPVAMVGMICGLIVVEPDLGSTVVIGLVFVGMMYLAGARISHLGYLGVPMIVGVG
                     SLIWMSPERWERMTTFLNPFADRQGAGYQLVQSILALENGGLFGVGLGQGKQKLMFLP
                     EGHTDFVLALVGEELGLVGTCGLLALFAILVCKGFRVAALAPDLFGRYLALGITMLLG
                     IQALINAGVVSGLLPTKGLTLPLVSYGGSSLLVTMLALGILLSVARQGRAGP"
     gene            69156..70244
                     /gene="murG"
                     /locus_tag="PKM72_00295"
     CDS             69156..70244
                     /gene="murG"
                     /locus_tag="PKM72_00295"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNP59239.1"
                     /translation="MRVVIAAGGTGGHMFPAIAYAKEFQQHDPGGSIVFVGTGKSLEG
                     EILGKEGFGFEPITVEGFVGRGVVGRLRSLILLPNSLWRAVRILRGHHADLVIGTGGY
                     FSPPVVVAAWLLNIPRVLLEPNAMPGLANRVLGPLADRIFLAFDSAKPFFSSSKVKVI
                     GTPIRKAFALERPPVPPQKISHLLIFGGSQGARAINSAMLNAVECSSRLREQVTITHQ
                     TGADDFERVKAGYAQLGVQVDVRPFLFDMPQELAKADLIVCRAGASTLAELSACGKVG
                     ILIPFPQATHNHQEMNARSMEAAGAARVLLQSDLTGQRLAEEIEQWISDIPRLQEMAG
                     RSWALRKIHATEQMVGECLSLVGRPVAS"
     gene            70567..71946
                     /gene="murC"
                     /locus_tag="PKM72_00300"
     CDS             70567..71946
                     /gene="murC"
                     /locus_tag="PKM72_00300"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNP59240.1"
                     /translation="MFRKIQHIHLVGIGGSGMSGIAEVLLTLGYKVTGSDVGVSDTIR
                     RLEELGGTVFIGHKESNVEGAQVVVVSSAIAGSNPEIRAARAKVIPVIPRAEMLAELM
                     RLKFGIAIAGAHGKTTTTSMVASILAQAGLDPTFVIGGKVNAMGTHARLGRSDLLIAE
                     ADESDGSFLRLSPSIVVVTNIDREHLDHYGNMEGLQEAFLEFINKIPFYGVAIVCADD
                     PWIRKLLPRVVKRYHTYGMSDFSGALTSDLYATEIETKSMGVEFRAHYRDQKLGPFRI
                     RIPGVHNVSNALAAIGVALELDVPADLIRAGLAAFAGVERRFQIRGEKNGIVVVDDYG
                     HHPTEIRATLAAARAAWQRPLIVVFQPHRFTRTRDLADEFSKAFEQADRVYVMDIYPA
                     GETPIPGINGQMMADTIRASGHPSVQWLNRDSGLISKLREELRDGDVLLTLGAGDVWK
                     VGTEVLESL"
     gene            71985..72905
                     /gene="murB"
                     /locus_tag="PKM72_00305"
     CDS             71985..72905
                     /gene="murB"
                     /locus_tag="PKM72_00305"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNP59241.1"
                     /translation="MKTGRPKWTAKRLQRAVQHVQGEVCWNEPLAPLLSLQVGGPADV
                     LVFPSDVEDVIRVVLGARTEGIPLVVLGGTNVVVRDKGIRGIVMQLKQLTGLQEEAGH
                     VVYAQAGVRLPRLMQFAVGHHLSGMEWAAGIPGTVGGGVVMNAGTRLGEMQEVLKGVD
                     LVNLRGHRVRIEASNMSFAYRKATLPKGIVVGAWVQLALSVKEQVEAKTKTYLRYRRE
                     TQPLTLPNAGSVFKNPPGDSAGRLVEAAGLKGVRVGDAEVSTKHANFIVNRGTATADQ
                     VLALIRKVRQTVARKFGVHLQLEWKIIGES"
     gene            72892..73953
                     /locus_tag="PKM72_00310"
     CDS             72892..73953
                     /locus_tag="PKM72_00310"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNP59242.1"
                     /translation="MENRRDARLDGGEEAIQSEWKAYHKVKKQGMMTQKSLRIGVLMG
                     GSSAEREISLKTGRSICDALTRRGYTVIPIEVDASLPHQIRAKKVSVAFLALHGPGGE
                     DGTVQGMLEVMKMPYTGSRVSASAVCMDKGLTRVVLQAAKVPVPPGMTLSGKMIPVRP
                     PATLGLPIVVKPCAEGSTFGVSIVRKPSQWKQALQKAYQFGEQAVVEKYIPGREVAVG
                     VFGNEVLPGVEIIVPGGFYDFTAKYGKAPTRYVCPASLSSKLEAALRDYSLRAYQALG
                     CRGAARIDFRIHTNGRPYILELNTIPGMTERSLLPMAAAQIGWDYDDLVERILRDALF
                     PKKMEASVRKAKTTRKSAS"
     gene            74022..74771
                     /locus_tag="PKM72_00315"
     CDS             74022..74771
                     /locus_tag="PKM72_00315"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNP59243.1"
                     /translation="MKAKTSSTALKVRRVVIGGVLACLIVVVLVGGRQVIRWADEWTE
                     IQQITVVGLDRVTRDEILTRLDLPPQTSLFSVDPELLATRLDSHPWIGSVAFERVFPH
                     SLVVQVTERQPAAVFGSPTEPYFLDAEGYLLPKGKAVAGTTLPIVDGVTSKFMTQHED
                     EGHRRAKQGIHIAELLSQQFSGRPRIDVSQPHTTVVDLPNVRFQFGREVEDQWQRFLV
                     LYPTVKDKIEGRSQEVDLRFAQKVIFRKRTL"
     gene            74775..76028
                     /gene="ftsA"
                     /locus_tag="PKM72_00320"
     CDS             74775..76028
                     /gene="ftsA"
                     /locus_tag="PKM72_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNP59244.1"
                     /translation="MTKKEHIVVGLDIGTTKICAVVGEIQDDRSIHVIGVGSHRSNGL
                     RKGMVVNMESTVESIKRAVEEAELMSAVQINSVYTGIAGSHIGSESAQGVVALKKREV
                     TKADVRRAVDTARACAVPAPDRQILHVLPREFIVDDQEGIRDPLGIAGSRLEVDVHIV
                     TGAVTSAQNLIRCVSRAGLDVVDIVLQPLASSAAVLTAEEKDLGVVMVDIGGGTTDIA
                     IFVEGCIRHSAVLPIGGSHLTGDLAMGLKTAPEEAEKIKLKYGVASSLFVNEDEGIEV
                     PSVGGRPPRVMPRALVAQILSPRVEEMFELVLREIRRAGYEGMLVAGGVLTGGTSLLP
                     GMAEVAEHVLNLSVRLGRPIGVEGLREIVSNPSYSTAVGLIVHSVSHENEFELVGPGA
                     GKGKKSGRWAGGVVGKMKGWIMNFF"
     gene            76141..77346
                     /gene="ftsZ"
                     /locus_tag="PKM72_00325"
     CDS             76141..77346
                     /gene="ftsZ"
                     /locus_tag="PKM72_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNP59245.1"
                     /translation="MFSLSEEEESGINIKVVGVGGAGCNAVNTMIEAGLSRVEFVVAN
                     TDLQALGRSLASYKIQLGPERTRGLGAGAKPEVGKESALESEHQIREALEGADMVFVT
                     AGMGGGTGTGGAPVAAKIAKELGILTVGVVTKPFQYEGNRRTSFAEEGIRELKKHVDS
                     LLIIPNQKLLGMVDKNTPLVEAFKIADDVLRQAIQGISDVITTPGLVNVDFADVRTVM
                     GYSGRAVMGMGTGRGPTRAGDAARQAIASPLLEDGSVEGARGLLLNITGGPNLTLHEV
                     NEASNIAREAADPQANIIVGQVINPDLGDELTVTVIATGFEQAESVLRFPSAHQPTLV
                     VEPVKKPALVAVPTENGGSNGSNGSEDLDRPTYLRRQTAARPIPEKNGLLVDDDWDVP
                     TFLRRKADN"
     gene            77351..78106
                     /gene="pgeF"
                     /locus_tag="PKM72_00330"
     CDS             77351..78106
                     /gene="pgeF"
                     /locus_tag="PKM72_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNP59246.1"
                     /translation="MSAEKRPLPVGTGVSDDGFTSHVIPVRHFFGTRSTPAGLHTHIQ
                     LGHLVSGPKEFPAIVSLKQIHSTRVVVIRPHRGLGALEQTEGDALVTNQPETLVVVRT
                     ADCVPVLLVEKARGVVAAIHAGWRGAVAGIVAETIRACVDEFGAKPEHMHLAIGPSIG
                     PCCYEVDEQVINPLRSRYPAWPGVLQETHEGKGVLDLKQLIYHQIRAGGVPDSQIGRV
                     DHCTHCRDDLFYSYRREGQVNGTMCSGIMLPAA"
     gene            78330..79070
                     /locus_tag="PKM72_00335"
     CDS             78330..79070
                     /locus_tag="PKM72_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNP59247.1"
                     /translation="MKDGHVSHSITHTIQMVKENIRQAALRVGRDPSGVRLVAATKTV
                     PAVNLDEAYWAGVRIFGENRLQEAQEKREVLGPREELVWHFIGRMQRRKLKDLVGNFS
                     LLHSVESLEQAERINAVAEKLGIQQAVLLEVNVGEEASKGGFTSQEVEARMEDLDRLP
                     HVEIQGLMTLPPWQENPEDVRPYFIQVSRLRDRLARQTWSRVRMTELSMGMSHDYEIA
                     IEEGATMVRIGTAIFGSRGKAVSPGTAG"
BASE COUNT        19557 a        16624 c        21784 g        21204 t
ORIGIN      
        1 ttctcaagaa agagaaagat attctgtccg ttcgcatggt tgatctcagc tagctagacg
       61 gtttctttct gcagactatc ttggccaaac atttcgagtt ggcgggttct cttccatacg
      121 cctcatgaaa ccactccgtt cgcttatccc cattggcaca gccaccttac ttcctcatac
      181 agcgcaaagc attcggaggt tggaggatca attattagcc caatgttcga cctggggcta
      241 tcaggaaatc attcttccta cctttgagta tcttgatgtc ctagccgtgg gcttggctcc
      301 tgaaatctta gagaaatgct ataaatttgc tgattgggca tcaggaagaa ttctggtctt
      361 gcgacctgat gccacggctc agattgccag gatggtggcc atgggccttg gaggagaaac
      421 tcttccctta cggttttcct atcggaccac agtttttcgg tatgaacccg aacatcgggg
      481 gcgagaccgc gaagtatttc aggtcggatt tgaaatgctt ggcaatgata cttctcaaag
      541 tgatgccgag gttgtgacat tactggctgg gcttctggaa gggattggat tgccagaatg
      601 gaagatttct cttggtcatg tcgggttttt taaagcccta ttggcccaat caggtctgtc
      661 tctgaatggg cgaaagcagg cagaaatcgc ggcagcccat aaagatctcc ctcagttgga
      721 aaagatatta gagactgaac ggttatcccg ttctaaggta aaggacatct tgcaggtgcc
      781 ggaacggtac ggccgtgatg aagtgttgga atggggaatg cgaattgcgg ggaaggattc
      841 gcaattgctt aaacccctac aacgtctgac gcaggtctat cggcaattga tagcgaatgg
      901 agtgcaagat cacattcttt tggatcttgg tgagtttcgt ggttttgact attacgatgg
      961 tgtggtcttt gatgttttca cctccacgtt cggcagtgaa atcggtggag gagggaggta
     1021 caatcatttg gttgggcggt ttggccgaaa cctttccgct attggattgg ggttggattt
     1081 ggaccgcgtg ttttccgcct tggagcgcgg aggaaaagtg ggcggcaacg ggttgcagcc
     1141 tgtgagaatt tttacttctc ctcatcaagc tgaggcatct tttgctcttg cccaacgatt
     1201 acggggggct gggatttgtg tgattgaaga aatgatagag ggttctccaa agtctgcttt
     1261 gaggtgggtc atcacggtgg gtcggcgtcg agggttgccg tgtgcattga tttttgaaga
     1321 aaggacctcc accccgcaat cggtgctttt attagaattc tcctcctttt ctcaaaaacc
     1381 ccggcaaacc cggataccag ttcaagaact tcctcaacgg ttacaaacat tcagccatga
     1441 caatatctga agcaccagag gttcgggttc ctcctcaaaa tctagaagcg gaacaatccg
     1501 ttcttggagc gatacttctt gataatgctg cgctcaatcg agcgatggaa atcttgtcgg
     1561 aggaggattt ttatcgaacg gggaaccgtg tggtgtatcg cggcatggtg gcattgtccg
     1621 aacgaaatca acctgttgat caaatcaccc tgacagacta tttacgagga accggcgaac
     1681 tcgaccagat agggggtgct tcgtatattg ccgaaattgt gcaagtggtt cccagtgccg
     1741 ccaatattcg gtatcacagt aatattgttc gcgataaatc cttgcttcgt ggcctcgtac
     1801 gaaccgccac cgacgtggtc atgcggggct atgaagggac gacggagacg aatgaacttc
     1861 tggagtttgc tgaacgggaa attttccggt tggctcaggg acatttagga ggcacgtttg
     1921 cgcccgttag tagtatcatt aaagacagtg ttgagattgt ggatcggttg tatagccgaa
     1981 aagagcggat caccggtgtg ccgactgggt ttacagacct ggataatttg ctggccgggc
     2041 ttcaaccatc cgatttaatt attgtggcgg gtaggccgag tatggggaag accagtttag
     2101 ccttggggat ggtcgaacat gcctcgcttc actccaatgc tgtagtcggc atttttagtt
     2161 tggaaatgtc tcgagcacaa ctggttttgc gcatgctgag ttctcaggct cgtctcgatt
     2221 ctcatgctct tcgtaccggg cagttgacca aacatgattg ggtggcctta actgaggcgg
     2281 ccggacgact tgagcaggct aaagtcttta ttgatgattc cggaaatttg accgttcagc
     2341 aaatgcgtgg aaaagcgcgg cggcttaagg ctgaacatgg attgagcctg ttgatcgttg
     2401 attatttgca actgatggaa gggagaggaa atgccgaatc ccgacagcag gaaatttcgg
     2461 atatctcccg ggccttaaaa ggactggcga aagaattaaa tattccggtt attgccctgt
     2521 cccagttgag tcgagcagta gaaaaccgga cggacaaaag gcccatgctt gccgacctac
     2581 gtgaatcggg agcaattgaa caagatgcgg atgtggtgat gtttatttat cgagatgaag
     2641 tttacaatcc tgattccgac gataagggca ttgcggaaat cctggtgaga aaacatcgga
     2701 atgggcccat tggagaagtg gatctgcaat ttcatgaccg gtatgccaaa tttaatgact
     2761 taacgagagt gcgtgaggct ggtatagtat aacgagttgt tgaggtaggg tttatgaaat
     2821 ctacaagagt gctagctatg aaggttcgtg ctgggcagac atttccaatg tggttgggtc
     2881 gtctgaggtc tctcttgtgg ggaattccgc tgttggtggt atcttgcggg acagctccct
     2941 ctcccacgct gaatggacaa gaagtgtcca tggtaagtcg agaaacttct cacccccagg
     3001 cctactatca cttccttcgt ggttcccttg ccgagttgaa taatgatgcc actacggcag
     3061 tagaggaata tcaggccggt ttggcttttg acacagattc gaacttcttg aaatttcgtc
     3121 ttgccaaact gcatttctcc atgtcgcatc tgacggaagc cttgggttta gcgagacaaa
     3181 ttcctgtgtc ggaggtttct caggcggcga tgtttttcga tttggcaaaa atctttgctg
     3241 gtggagggga tactgaacgg gccttggagg ttctcactga aggggaacga cactttccac
     3301 aggatgaacg gatatatatt tctcatggca cccttctgtt aaatatgaaa gagcttcaaa
     3361 aggctgaaga ggttttccat gaattattag ttcatgtgcc tggctcagcg gaagcgcatt
     3421 attttttggg cgtgattgct ctcgaggcta aaacgaagca ggaagccaag gcccattttc
     3481 aggaagccat tgctcttcat gccacctttg aacgggcgta tctgaagtta gtggcaattt
     3541 ctgaagaaga aaaggactct caggagggta ttgcactttt agagcgctat ttgattgaag
     3601 tgaatccgca tcatcgggaa ttccggttgc gggtaatccg tttatatatt ggccagcaaa
     3661 aaacggacaa agcattgaac catttggact attttctcca agaaaatcca gatgatttgc
     3721 atgcgcaaat tcttaaggct caaatctatg gtgaattagg caattttcct gctgcaatcg
     3781 aggagttaaa tgccatcttg cgggtgagac caaacgaatt gcgagttcgt gatttcctgg
     3841 gattgttata tgaggaaatg aaggactatg accgcgcgat tcaagcgtat cgaactaatg
     3901 ttcaaatgga ttccacattt ttcgatagta ttctgcattt aggctttgtg tcctatcggt
     3961 taaaacgcaa ccaagaggcg ctttcatatt tggatcaggc cgttaatctc aatcctagaa
     4021 ggccagaacc gtatttatta ttggggttga cccattttca gatggaagat tatcacaagg
     4081 ccagggccag attggaggag ggcattcacc aggacccctc aaatgccgag ctccatttta
     4141 atttaggcac agcctatgac aaattagacc ttttcgataa agtggttcgt gaaatggagc
     4201 agactcttga gttggatccg gaacatgcgg atgccttgaa ttatttgggt tatagctatg
     4261 ccgatcgtgg catcaatggg gagcaggcct tggctcttac gcaacgtgct gtcgcgttaa
     4321 aaccccataa cgggtattac gtggatagtc tagcttgggc gctttacaaa cttggacgca
     4381 ttgaagaagc gctggaaacg attcagcgtg cggtctcttt agtgtccgat gatccagtga
     4441 tttatgaaca tatgggggat attttcctaa gacaagatga acgagataag gcacgagagg
     4501 catggcttca ttcactgcaa ctcgattcaa ccaataaacc gctggggaag cggtttcgtg
     4561 acgccggttt tggggaaccg attcctaccc tttcccacaa gtctaccttg accccctaac
     4621 atctcaccat cctaaaattt gacgtgggtc aatcaataat taagaggggg cttcaggtta
     4681 ttttaaaggt cgctacgtgg cggttttgat gaattttgtt ttaagtcatt attcgcctca
     4741 tggtctgatt cttaaagttt tggttctttc tggcgaataa atagtcacgc cttttcttta
     4801 ttggccttgc gtgacgattg atacttccaa cctttgtgtc gctgatatag ccatttttat
     4861 gagattagtt gtgtttggcg tatattcttt gccgtcttta aggtcgtgtt gataatcatc
     4921 aaagccctgg ctacttggaa aatccaggtg taccatcatc cgagggtaag ataaataaca
     4981 atctattgga gaatagtcct ccatggaggt ttccaggatg ctaactcaac tcaatgacca
     5041 aagaggattc agtttgactg agctgatgat tgtggtggca attattggga ttttggcgac
     5101 catagccatc ccaaattttt tgcgttacca agccaaggcc aagcaaacag aggcaaaaag
     5161 caatttggtg gcaatccata ccgccgaaat tgcatatttc gctgagaata atgggtatat
     5221 agatgatttt aatgcgattg gatttgccat gagtgggtct tcgcaaagat atttttataa
     5281 aattggcaat gccaatcttg ggacattacc tcctggttgt accgacccga atttagattc
     5341 tgtaagtgca ttgggattta ctgcggttgc aattggaaat attgatggag atgcaacctg
     5401 cgacgtgtgg accattaatg agggaaaggt catcacgaat gtgacaaatg atgtgtcttc
     5461 ctagtttttt tgtgtcatgg cgcgatgcat ttaacagtaa ccaatacaag gaaagttgat
     5521 ttcctaaaac tttaactgaa tcgttttaag aaacatgcct caagaggagg gcaaaagtgg
     5581 atatgctcga taaaattaaa aatcggtcag ccaaggttgg agtgattgga ctaggatatg
     5641 tagggcttcc cttagccgta cttcaggcca aggccgggta ccaggtgttt ggggtggatg
     5701 aagtagcagc caaggtagaa atggtgaacc aggggcggaa ttatatattg gatgtggtgg
     5761 actctgattt gcgagaagtc gttgagcaaa aaaaattagc ggcgacaacc gatttctctg
     5821 tgttacggca gtgtgatgta atccttattt gtgtgccgac tcctctgacc aaaaataaag
     5881 aaccggatat ttccgccatt gtgaaagttc ttgggcatct tgccgataat tcacatccaa
     5941 atatgctcgt tgtcttagaa agtacaacat atcctgggac aactgaggag gttattttgc
     6001 ccgccttaac cgcaacgggc ttaacggttg gaaaggatct gtttgttgca ttttctccag
     6061 aaagagttga ccctggaaat cctgagttta aaacccataa cacatttaaa ttggttggtg
     6121 gtgtgacaga ggcatgcggg gaagttgcca aaaccttcta tgagcaatcc atcgtgaaaa
     6181 tctttcttct cagctcccca cgggtagcgg aaatgtctaa ggtatttgaa aatgtttttc
     6241 gctcagtaaa tattgcttta gtgaatgagc tcactctgct gtgtgaccga atgaacatta
     6301 atgtatatga agtgattgat gctgcggcca cgaagaattt cggtttcatg cctttttatc
     6361 ctggcccagg agtgggaggc cattgcattc cgcttgatcc atattattta gcgtggaagt
     6421 caaaagagta tgatgtccat accaggttca ttgaactggc tggggaaatc aacgaaaata
     6481 tgccttattt tgtaatgaat aaactgcagc gggttttaaa tcaacgaggg aaatgcttaa
     6541 aaggttcaac catcctaatt ttgggagtaa cctataaagc cgacattgaa gacccacgtg
     6601 aatcgcctgc cacaaaagtc atggagttgc tgcaacagga aggtgcggat ttgcaatatt
     6661 ctgaccccta taccccttcc ctcattattc aaggacatga atataaatct caacagataa
     6721 atgccgaatt gttgaagggc agttcctgtg cgctggtttt gaccgcgcat tctgcctttg
     6781 attaccagtt aattgttgat cacgctccgg tggtatttga cacgcggaat ggaactcgtc
     6841 aggtgaagca tcaccgtgat cgtattgttc tgctttcaac ctagggaggg tcttaaatca
     6901 gagcctattg acgggtgaat agccatcggg ttgacagtct agtctcgttt cgttataaag
     6961 ccctgtttaa gcagggcttt ttgcgtcctg tgaaaatcac attggttgtt aacattcccc
     7021 caaaatcgca ttatagaata ttgcatctct gaaagaacgt ctgaatgaag ttgctcaagc
     7081 aattcttggt acggttatcg ggtaatattt ttgaaaccct gaccccaaag ttaataccct
     7141 atcgaggaaa gcaaaaacca ccgcccctta agctggtttc tcataacctg catcctgacg
     7201 tttctcacga tcctactctc caacgtacgg ccaatcttgg gcatatgagt gctctggagt
     7261 cagctcttca aaaaggggag gactggttat tgggcatgca agatcctgaa cgaggatttt
     7321 gggttcagga attggaagcg gatacgaccc tgacatctga atatgtaatg cttcgacggt
     7381 ttttggatat ggtggatcct gaaagagagc ggaaagctac tcggtattta cttcataccc
     7441 agcttgagga tggcgggtgg ccgatttttt ccggagggcc ttcagaaatc agtgcatcgg
     7501 taaaagcgta ttttgcgctc aaactggctg gggtttcttc tgatgagcct gtgatgcaac
     7561 gtgcgaggga tttaatcctt ggaaaaggtg gtgttgttca ggccaatgtc ttcaccaaaa
     7621 ttaccttagc attattcggg caatatgatt ggcgggggat accgtgtatg cctcctgaaa
     7681 tattcttagc cccgagatgg ttttatttta atttatatgc ggtttcgtat tggtctcgtg
     7741 tagtcattat cccactttta attgtttttg cccatcggcc cgtttgcgca atctccaagg
     7801 aacaaggaat tgatgaactt tttcttcagc ctcgtcagga ggtggattat gctaatgtgc
     7861 caccgctgca tcgggattcc accatcctaa gttggcggaa tttctttgtg tgggtcgatg
     7921 ggctgcttcg tttatatgag gcctacccga taaaggccct taggaaaaag gcgttgaaaa
     7981 gcgcacaggc gtgggtaatt gaacatatgg acggagaagg tgggttaggg gccatttatc
     8041 ccgctatggc ctattcaatt tatgcacttc gagctttggg atattctacg gatcatccgt
     8101 tagttcagaa ggcattgagg gaaattgaag accttgaagt ctactcaaac tcgggtaata
     8161 ccccaacccc tactatgttg catttacagc cttgtcattc tccagtgtgg gatacagcgt
     8221 taattattaa taccctgatc gaacggggcc ttgcgctgga ccattcgtca ttgcgacggg
     8281 cagatgcctg gttgcgatcc cgtcaatgtc aaaaagtggg agattgggct gtgtcagcac
     8341 cggaagctcg atctggtgga tgggcgttcc aatttgaaaa cgaatggtat cccgatgttg
     8401 atgataccgc agctgtgata acggggcttg ccaagatcca ccctgtcgag ttgtccggtt
     8461 ctgatggggc cattcaacga ggatttcgtt gggctttggc gttacagagt tctgatggtg
     8521 gatggggggc atatgataag gataacaata aattaatttt caataagatt ccttttgcgg
     8581 atcatcaggc tctcttggat cctagtacag ccgatttaac cggtcgatgt ttggaaatgt
     8641 tgggaacgtt aggttatgac cagtctcacc cggctgtgaa ccctgcaatt gagttcttac
     8701 gcaaggaaca agagccaaac ggaagttggt atggtcgatg gggggtcaat tatatttatg
     8761 ggacttggtg tgtcctttca ggtcttcgtg caataggcat cgatatgacg gtgccctgga
     8821 tccagcaggc cgtgatttgg ctggagtccg ttcagaaccc tgacgggggt tggggggaaa
     8881 cctgtgattc ctatgctgac ccgcagaagg cgggtcgagg ggaaagtggg gcatctcaaa
     8941 cagcctgggc tcttatggca cttcttcagg ccggagaatc ggactccatt tgtgttgtcc
     9001 ggggagttaa ttggctcatt cgccatcaac gtgaaaatgg tgtttgggag gaaccgtttc
     9061 atacaggtac cgggttcccc agagtttttt atttacggta tcatggttat tttaagtatt
     9121 tccccatatg ggccctgagt atgtaccgga atgtaaaagt aaccggggag gcaagggccg
     9181 accatttacg aaaggccgca caagtggcca gacacatcag caagcttgtc taatttccgt
     9241 cctgactgct cgtttttggc atatctccct ttggaaaatg atatggcagg agccttttct
     9301 gaccagaatc gtcatattga cagcaacccg agtagagttt aacgcaattg gccgagttct
     9361 tcctaaaggg agctctgtta aatgcgtagg ccatgctgga ttagagagtc gggctgagtc
     9421 gacccatgta ttggtgattc agacaggaat gggtcccgac aaggcccgat ctattgccca
     9481 gaaaatttta gagagcgaag tctgcgatgt cgttatttct acaggttttg ccggggctct
     9541 taattcctca ccaatcggat cgctggtgat tggtcaggat gttcttctat tggaacaatc
     9601 tggagatttg atgtctgatt ttgttctacc accaatcaag tgccatcctg attgggtcaa
     9661 tgcgtcctta ggcatccaat tgatagatgg gtatcctctt caaagtggcc gctttgtgac
     9721 aacggatcaa gtgcttacac aggcttcaca aaaaggtatt cttgggaaac ggtctggagc
     9781 cgtggctgtc gatatggaga gtggagcgat tgggcaagtg gcgaagcatc atggtcttcc
     9841 ttttctgatt gttcgttcga tctctgatgg tattaacgag gatttgccgg tagacttcaa
     9901 tatctttctc aaaccatttg gatgggttaa aggagtagca ctggtcctat ccactcctcg
     9961 gtgttggaag ggattatttc gtttgtatca gaattccaag caggcgggta tccagctttc
    10021 tagttttttt gaaaaatttt ttgccattat tttcacccca acgttttcta tggccatcaa
    10081 taaatctcgc tcggaaattt catgatattg ttgcaacaca ttgggaaacg gactcttttc
    10141 ttgatagaag aaatgggggc catgtttgtg ttcttgatcc ggacgtttgg atggttattc
    10201 cgaccgccag tgaggatcgt tcaaattgtc aaacaaatgt atgttgtggg gttcaagtcg
    10261 tcctttgttg tgatcctgac ggcgttattt accgggatgg tattagctct tcaaggctat
    10321 tattccctga gaaagtttgg gtctgaaggt ttattagggt ctgctgtggc aataagcatg
    10381 atccgtgaac tgggccctgt attggctgca ttgatggtga cggctcgtgc gggatccgcc
    10441 atgaccgcag aaattgggat tatgcgtatt accgagcaga ttgacgcatt ggagaccatg
    10501 gcgatcaatt ccttgcaata tttgattact ccgaaaattg tcgcggctct tatttccgta
    10561 cccttgttgg ttgctatatt tgacgttgtg ggaatttggg gagggtatct ggttggagtc
    10621 aagttgctcg gagtcagcgg gagctcatac tggagttcaa tcgaatcagc ggtggaatgg
    10681 aaagatgtct atgggggaat attaaagtcg atcagctttg gcctgattat tagctggatc
    10741 tgctgttata aaggttatta tacgagaatg agttccgagg gactgggaaa agctaccact
    10801 gaatctgtgg tgttagcctc ggttctcatt ttggtgtggg attatttttt aacgtctgta
    10861 cttatgtaac gttaccattc tatgagtcta ccgattatcg ttaagctggc cgaagtagaa
    10921 aaagcatttg gcgagcagtc tgtgcttaaa ggggtcacac tggacattcc taagggtaaa
    10981 attaccacga tcatcggtcc aagcggtgag gggaaaagtg tgatgctcaa acacatcatt
    11041 ggattggttc gtcctgatcg aggcaagatc atcgttgatg gaacagacct tacacgaatt
    11101 aatgatcggg aattaaatga catacgcaaa aaatttgcta tgttgttcca atcggccgcc
    11161 ttatttgatt ccatgaatgt atttgataac gtcgcgtttc ccctgcggga aaaacgaagg
    11221 gctgctgaac aggatatatg tcgattggtt caagaaatgc tggaaagggt ggagttggga
    11281 aacatgggtc ataaatttcc agcagaattg agcggaggga tgaagaagcg tgcaggtctg
    11341 gcgagagctc tggtgatgag gcctgagatt attttatttg atgaaccgac gacgggactg
    11401 gatccactga tggcccatgc aatccacaag ctcattttag ctatgcaaaa aacatttggg
    11461 ttcacgggga ttatggtgag tcaccaaatt ccagagatct ttcctatttc agattgggtt
    11521 gctatgttaa agaatggaaa gatcattgct atgagtccat cggatgaatt ccagcagact
    11581 gccgatcccg tcgttcgtga gtttatttca gtcaataatg gtattcatct tgctcctgcc
    11641 tgaatcgtta atctggatag tcgtttaagg agtcatatca aaatttatgt cacgctgaaa
    11701 tatgagaatt attcttcctg gcccttgcct taatgaaata tcacttaacc taactgaatt
    11761 attatgatgg aacgtggaaa acttgaatta attgtcggca tatttgtgtt ggtgggggtc
    11821 atttgtcttg ggtatttggc cattaaactt ggaaaacttg aattggttgg tggtgattat
    11881 tacgaactcc aggccgaatt ctcttctaca tccgggttaa aaaacggagc ttctgtagaa
    11941 attgcgggag tggaagtggg ccgggtaaag aatatcggtc tgaaacaaga tcgggctcaa
    12001 gtggtccttg cgatccagga cgggatcacc gtgtttgacg atgcgattgc ttcaataaaa
    12061 actcggggga ttatcggaga aaaattcatg gaattatctc ccgggggtgc tggagaacgc
    12121 ctggaaactg gtggaaccat tatagatact gaatctggaa ttgatttgga gcaggtgatt
    12181 agtcaattta tccacggcaa tgttgaatga ttattcgttc agtgaaatag aggtggggga
    12241 atatgcaaag ggaattggtt cggaggcctg aatcaaccat gaagatggga gcaaatgcat
    12301 ctcgaaggtg tcagtggagc aattctttaa gaaatgtggc gattattttt tttggttggg
    12361 tggtagtttc tcttgcatgc ttaactgttg catggggagg aggaactcca accggagcgg
    12421 tcaaggagac cgttgatcag gtatttgtgg tgctgagaga tcaagcgttg aaggatcctg
    12481 ggaaagagat ggagcgccgg gcgaagcttg aagaaatcat tgggcggcga tttgactatg
    12541 ccgaaatggc caagcgcacc ctggctgctc agtggaaggg attaagtcca gagcagcaac
    12601 aagaatttgt aactttattt cagcagtttt tggcaaattc ctacgttggt aatgtagacg
    12661 ggtattcggg ggaagaggtg gaatacctaa aagaacgcga aaagggagga tttgctgaag
    12721 tccagaccaa agtggtgtct ccaaaagtgc agataccctt ggattatcgt ctgttgcaaa
    12781 aaaatgggga gtggcgggta tatgacgtgg taattgatgg agtgagcttg atgaaaaatt
    12841 accgcggaca attttctcgc attattaact cctcatcgtt tgaggccctt ctcgagaaac
    12901 ttcgttcaaa agccgacctg gggacttcgt cctaaggagt cggctttgtg atatcgcttc
    12961 gtttctgggg cctggcgtta tttcgcctgg ctgtgggcgg cgctatcctt ccctcatcaa
    13021 acgcactcgc cgagaattcg ttttttgtgg tccctgcggt ttcttcttca aaaaatgatg
    13081 ggcaggactt cgggctgatt acaccgatat tgaattcaga tacggaaggg aacctccgtt
    13141 ctctcgttgc cccgatgctg atccacaatt cctttttagg agttcgggga accttgaact
    13201 attttcatta ttggtctggc gggaggcaaa tggaggcggt cggttcgtat accgaggaaa
    13261 ttgaacgaaa gctgaaattt cgcttccagg accctgggtt tatcgaaggg ttgttcttcg
    13321 tggatattgg tgcacaattt tttaaaaatg cgaccaagcg gttttatggc ttagggcaaa
    13381 tcactcctca agataatgaa acaaactata ccggccgtga aattcaagtc cattggaact
    13441 ttggaattca tttgaatgat gtgacgcgtt tggcggttaa ccaaaggttt cgagatgtag
    13501 aaattcaacc gggaggcgtt gatagcgaac catatgccaa ggatcggttt ccaggagatc
    13561 cgggaatgaa aggcgcgacg attttggctc accgtcttgt attccagtat gattccaggg
    13621 ataaccttaa tacccccaca gcagggactc gattcgaagc atttggagaa ttggctcaga
    13681 actttgatgc tggaaaaaat gacgatatgt tgtactttct gtcagggttt gatatcagac
    13741 acctgattcc gagtccctct aagcgatata tttttgtggc aagggcgatg gtgcaattaa
    13801 gttttgggag cgatattccc ttttacgagc aaagttcttt gggtggagaa gataacctca
    13861 ggggctttgg gagggataga tttatagaca agcatatggt ggcatttaat gttgaggagc
    13921 gtattcatct atttgggatg aaaatgttta acgtcggtat cgaatgtgaa ttagcgccat
    13981 ttgttgatat ggggcggacc tatccaaatt tcgaatttcg ccagttccat gactgggagg
    14041 ttacgcctgg agtgggattc cgagccatcg ttcgtcccaa tgtcatggcc cgcgtcgatt
    14101 gggggtatag taaggagggt ggagccgtgt ttgcggggct caattatcct ttctgatgat
    14161 aatggttaat tcgctatgca gggaaaatat gtgctgacaa tatctgaaaa tatgaatcaa
    14221 tatgtgcgtc taagtttatt ctcgctaatg acgggactcc tctttctccc ttctggggca
    14281 ttgttgggtg aggaggtaac cgatcagcaa tatcccgaga gccttgaaaa ggagctgggg
    14341 accttgatga gggagggagc tgcaaattca accgacctga ctcgcttgct tcggttggcc
    14401 ggcttgtatc tggatctggg ttatggggtg tacattgatc gggagcagaa gttagcagcg
    14461 ttccaggagg gagccagggt tgcccaaaaa gcccttgatc ttcgtgagtc ttctgcggag
    14521 gcccattttt tgtatgctgc caatcttggg agtgctgcgg agttggaggg tttggtctcg
    14581 gcggcattga caattcagaa gttgaagaaa catgtgcatc gagtgttgga acttgatccg
    14641 gaaaatgttc aggctcacca tatgttaggg cgaatgtatg aggaattgcc ttgggtctta
    14701 ggaggcgatc aggagttagc cgaggatcac ttgaaaatgg cggtttctct cgatagtcat
    14761 tatgcaccgg cggggctgga cctcgcgaag tggtacctga agcacgacca aacattggag
    14821 gcggtgaggg agctaacgcg tgttgttaac acgccccctc ttaaaaagag gtggatatgg
    14881 gagcggattc atcgaccgga agcacaagac ttactgcagc agattcttag tcaggggaat
    14941 gttgatcgtt ccaatgatca ttcctaaagt taggtatcta aatgcttgac agggtatagc
    15001 agctatggga agattttgtt tattcttatc ggaattgggg tatttttagt ttttccctta
    15061 caacaggcta tgtttatcaa gaaaggatga gcaatgtcct tgctaaagag gattgaaagt
    15121 ccagcagatt taaagaaact ttcccgtgag caattccctg aactctgtca ggaaattcgg
    15181 gaactcatta tagaagtgat ttcccatgtt ggtgggcatt tagcctccaa tttaggcgtg
    15241 gttgagttga ccgtggcttt gcaatattta ttgaatactc ctgacgataa aatcgtctgg
    15301 gataccggca atcaggctta tacacataaa ttattaacgg gtcggcgtga acaattccat
    15361 acggtccgac agttcggggg aataagcgga ttttgtaaaa gagaggaaag tatttatgat
    15421 acgtttaatg ccggacatgc cgggaccggg gtgtccgctg cggtgggctt tgttgaggct
    15481 cgtgagcaat taggaaagtc caataaggtg gtttgcgtcg taggggacgg tgccctcact
    15541 gccggaatga ccttggaagg gcttcagcat gccggagata tgggccgaga ctgtgtcgtg
    15601 atcctgaatg ataatcaaat gtcgatttcc aaaaatgtcg gggccatttc cgcctacctt
    15661 aatcggacct ttaccgggga attctataca aggcttcggg aagaaacctc tcaactcctt
    15721 gaaactattc ctcaaattgg ggaacccgtg aagcgaatgg cgattcgggc tgaagagctg
    15781 gccaaaggct ttctgctgcc aggcttgctt tttgaagagt taggatttcg atatgtgggt
    15841 ccgattgatg gacataattt tgagcattta ttgccgactc tggagaatgt tctaaaatta
    15901 aaagggccaa ctctcctgca tgtgattacc aaaaaaggat tagggtttca gccggccatg
    15961 aagaatcctg tttggtttca tgcctgttca ccctttgatt ccaagactgg gcaaccggtg
    16021 aaaaagcccg gtcatccctc gtatagttcc attgctgcca aaacactgat tcgtcaagcc
    16081 aaaaaagata accgggtggt ggtcattacg gctgctatgt gcgagggaac aggaatggct
    16141 gagtttgaga aggagtttcc ttccaggtta attgatgtag ggattgctga acaacatgcc
    16201 gtgacgtatg cggctggtct cgccgctgat aatatgcggc cggttatctg tatgtactct
    16261 acatttctgc aacgggcata tgatcaggtt gtgcatgatg tggcaaccca aaatcttccg
    16321 gtgacctttt gtattgatcg gggaggattg gtggcggaag acggaaccac gcatcatggg
    16381 gcttttgatt ttgcgtttct gcggcatgtt cccaatatgg tgattatggc accaaaggat
    16441 gaaaatgaac ttcagcatat ggtccaaaca ggactggtgc atgatgggcc agtggcggtt
    16501 agatatcctc gcggcagtag cctgggggtg ccattagatt ctgagcccac tcctctttca
    16561 attggtcagg gtgaactgtt gtctgatggg catgatgtgg cgattatcgc gattggagtg
    16621 atggtttcgg aggctatgaa agcagcggag cgtttagaag aagagggcgt gtcggtggcg
    16681 gtcataaatg cacggtttgt gaagcctcta gataaaaatc ttattcggga tattgccaaa
    16741 aaagtaaaat gtttaattac gattgaagaa ggttgccgga tgggcggttt tggatcggct
    16801 gtattagagt ttttatcaga ggaagaatgt tggagtatgc cgaccaaggt gctcgggttg
    16861 ccagattggt atattgaaca gggtcctcag gatttgctcc gggaaaagta tggcctcacg
    16921 gcggatggta tttatgatca ggccaaggcg ttgcatgacc gggtttccct tcaagccgca
    16981 atacgttaga ttattttcgt gtatatttct cgaagaaaaa caaagcaaat ccaggttgga
    17041 tcggtcaaaa ttggtggcaa tgcccccata tcggttcagt ctatgacgat tcctcatccg
    17101 agggatgttg ctgggacggt agagcaaatt caacagctag agaaagctgg gtgtgaatta
    17161 atcagggtgg ccgtgcctga tatggaagcg gctgatgcgc ttccaaaaat aaaatcacaa
    17221 acgaccctac ccttgatcgc ggatattcat ttcgaccatc gtctggcatt aaaagccgct
    17281 aaagtcgtgg attgcgtcag aattaatcca gggaatattg ggccgtggtg gaaaaccgaa
    17341 gaggtgattc aggccgtcaa tgactatggt attccactca gaattggagt gaatgggggc
    17401 tcgttagaga agcatcttct tgagaaatac ggctatccca cagctgaagc tcttgctgaa
    17461 tcggcgctga atgcagtcca tgcgttggag gatgttggat ttaccaatat gaaggtgtcc
    17521 ctgaaggcct ctgacgtgca catggcaatg gacgcctatt ggttgtttgc ccaacagtcc
    17581 aattaccctc tgcatatcgg cattactgaa gcaggaactg caatgactgg cgctgtgaag
    17641 tctgctatag ggttaggttg gctgctttct cagggtatcg gcgataccct ccgggtttcg
    17701 ttagcagccg atcctgtgga ggaagtcaag gtgggttttg aaatcttgaa atcgctggag
    17761 cttcgtcacc gaggagtcaa tgtgattgct tgtccgacat gtgggcgagt tgaaattgat
    17821 gtagtgaaaa tggcgaacga attagagcat cgactggggc atattaccac cccaatcacc
    17881 gtgtctgtct taggttgtgt ggtgaatgga atcggcgaag ggaaagaagc tgatattgga
    17941 atagccggag gacaaggcgt agggattctc tttaaaaaag ggaaattgta taaacgtgtg
    18001 ccatctgaag agcttcttca cactttaatt gaagaggtgg aattgatggc caaggagcaa
    18061 ggtggtcaag agccgagcat caatattcct gagacacctg atgtcctttc cggtaatccc
    18121 tcctctatgg aagatctttc cttaaaatca atacggtctg tttcaagaga acttcccgtg
    18181 ttgcctcgtc agtaatggtt ccagcccttc caagactaaa gaaagagaaa gccagatctt
    18241 ctcttgtaca agagaaaata gacttcttat aatgatcccg tcatggcgcc gtacccaagt
    18301 ggctaaggga gcagtctgca aaactgcgat tcagcggttc gaacccgctc ggcgcctcca
    18361 tttattcggg ttttatcaga cgtaggtgtt aggtttttgc ggggaaaaag aagtagacgc
    18421 tcttgaaatt tgtcaattac ctccaggagt aaaataacaa tgtcggtacc tgtgtcccaa
    18481 atgtacaccg tagctcatta tgctctctca cagcacttgc ggggtgtgaa gcgataccct
    18541 cttgtgatga tgttggaacc gctctttcgg tgtaatctgg aatgtgcagg atgtgggaaa
    18601 atacaatacc cggaccatat tttgaatcga cgcctgacgc ctgaacaatg tttggctgcg
    18661 gctgatgaat gtggtgcgcc aattgtgagt attcctgggg gagagcccct cattcatcct
    18721 gaaatgccag ctattgccga agggcttgtg aggcggaaaa agtatgttta tctctgtaca
    18781 aatgccattc tgcttgaaag aaaattacag gactacactc cctccaaata ccttaccttc
    18841 agcatccata tggatggcct caaggaagaa catgaccatg ccgtttgtcg agatggcgtc
    18901 tatgacgtgg ctgtgaaagc cattaaagcc gctttggcta aaggttttcg cgtaacgacc
    18961 aatacgacat tatttaatga tgcggcgcct gctcgtgtga gaagattttt tgatgagatg
    19021 atggagttgg gtgtggaagg catgatgatc tctcctgggt atagttatga aaaggcgccg
    19081 gatcagcata gttttttaag ccgtgaacga acgcattcgt tatttgcgca attgctggct
    19141 caacgaaaac gcagttggca gttcaatcaa tctcccttat tccttgaatt tctgatgggg
    19201 aagcgagagt atgaatgcac cccttggggg aatcccacgt acaatatttt tgggtggcag
    19261 cgcccctgtt atttattgca ggatgggtac gtatccactt ttcgagaatt aatggaagaa
    19321 acggagtggg agcgttatgg atcgggacga catgaacaat gccgcgattg catggtgcat
    19381 tgtggatatg aagcttcggc tgtgaaagat accttcagtt catggggtgg gtttttcggc
    19441 accgtcaggg caaccctgtt tcccaatgct gtgtgatctc ctattttttg ttttttcttt
    19501 cccttcctca attccgatca tctggtaatc gtctcaacgc cttgcgggaa gtaaagggcc
    19561 attatggatt cagatatgtc ctcaatcgat tctcctcaat cctgggaagg tcggtgtctg
    19621 caacctttga atgagaaaga attgcacgaa gccatgaatc tcgtgtttga ttataggggc
    19681 gatgtgacga ttcaactcaa aaatggggag caggttgtgg gatttgtatt tgatcgtcag
    19741 gaggaggctc ctcagccgta tatacagcta ttcctgccag gggatcagca tccgcggatg
    19801 gttttatacc aagatgtggc tggaatcgaa ttttcaggtc aagatacggc gtttggtcga
    19861 tcgtgggagg actgggcgca aaagtggaag aagtctgagt ccagctctta acaatcttaa
    19921 caatactact attcccgcaa gcattgtttt cccttcatcg ttctgaatca tccaaataat
    19981 tcaaaacagc ttttgcattt aatccgattc taggtgggat gggaaatttt tttctctctc
    20041 gactcattca ctggtctgtt tattctccaa aaatacaggc atggaaaggg taaagagccc
    20101 gcaagaacgt aggaaataat tgacggttgg cttctgactg tgatagaagt cctgaacttt
    20161 tttgggccca agagacctat gtagcccaag ggattttgaa taggaaaaaa caattgttcc
    20221 ggtcagtctg tttatgggct tcagaggtag accatcattc ttggattgag gtcagaattt
    20281 gtggaaatga gatggaatgg ccttgaggcg gtcgttgttt ccgtgaggga agctctggca
    20341 gatattttgg agaacgggga gtcaggattg atgttttata gaaggttgtc cttcactaaa
    20401 aagataagga tcggaagaag taatttttag aaggagtgat tctggtcttt aagtctagag
    20461 gggcaaagtt taggtgaggt aatgtggaat ttggcaaacc aagattcagg taaacgaggg
    20521 ggaacaatga acgagtttca atttgtaaaa agtcttttag ttagtggttt ggctgtcatg
    20581 ggcctgtcga tgtcagcatg tggtggtggg ggtgaaggtc cgccgcaacc tcctcctcct
    20641 gctcctccgg aatatgcaga taaacatatg ccgtcgggtt attggaataa tccggagatc
    20701 cttgcagaag gggaagcgat ttttaccggt cagcagaata ttgatgtgaa ttgcgcgagt
    20761 tgccatggta aagatggaaa gcctgtgaag gctggtgctc gcgatttccg gcgaactgag
    20821 caaatggagc tatattcgga tgcggtatgg ttttggcgag tgtctgaggg ggtatctggt
    20881 acaaagatga aagcttggaa gagcaaactt tcagaggatg ccatttggaa ggtaattgcg
    20941 tatgaagcca gttttggact caagggcaag gaatatgatg ttgcgaaagg tcaatgggtt
    21001 ccttcaggag gagctggtgc cgctcctgct gccgcaccac ctgcggatgg agagcctgaa
    21061 aaagcggcag agtaatccag gaattgaatg aaaaagaagg agggcgggga cccttgaaaa
    21121 cactgaatcg cagtctatgg atatttctag cgttgggcct aagtggagtg gctactgcct
    21181 ttgggcaggt tccagatgct ccacttgtag attttcccta ttcggggaat cgaacggccg
    21241 tatgggttgt cgcccaactt catatattat ttgcggcgtt tattctaggc gctccgatat
    21301 ttgcggttgt agctgaatgg cttgggtata agaataacga tccgaagtat gaccgtctgg
    21361 ccaaggaagt cattaaggtt acagtcatcc tgtatagtat gacagcctta actggaggtt
    21421 tgttcatatt tgtcctcctg gggacgtatc ctgacttttc gacatggttg atcaagcatt
    21481 ttttccttgt ctttgcggtc atttaccctc tgctgtttat ccttgaaaca ataattttgt
    21541 atacctattt ttattcatgg gattccatga agggagcgaa aaaaggacgc catatcgctc
    21601 ttggaatcct attgaatata gtgggaactg tgacactctt tgtcattgat ggaccgaccg
    21661 catttatgaa tacgccggct aaagctgtgg aaggcgtgtc attggttgag tttatccaga
    21721 ctgccagttt gtgggacaag atggctaatt ttagctggat gcccctcaac cttcatcgac
    21781 tggtaggaaa tgtgacgttt ggtggattta ttgccggact tattgcggct tatatgttta
    21841 tgggatcaaa aacagaagag gagagggcat attacgactg gatgggtttt gtgggaaaca
    21901 tgatcggcgt tggggcgctg ttgctccttc cattcatggg ttacctcctg gcctacgagt
    21961 tgtgtgatta cgacgcctcc atttgtccct acatgatggc tgaccaattg tcgatgtttt
    22021 ttgagatgca gggggcaatg atcggcctga tctttctggc cagtaattat tatatctggt
    22081 taagtcttaa gcgaatacag ggtgtagagc aggtacggat atctggattc gttgccgtgg
    22141 tggtggtgtt tatgccggcc atcatgggat ttacctggaa aatgttccct ccgcctgagt
    22201 ggcagtcttt gattgttcta ggaattctgg tcattttgcc tgttgtgctt gggaaagttc
    22261 ccggtcttaa gaattttacg gtgtcggctt tcaccatgat taaggttggt tttttaatga
    22321 ttgtggtggc ggatgcgatt tggatgaccc ctcatggttt tgttccgacc caaggcctgg
    22381 ccactgagga attggaattg ccgtcatggg cgagcgagtt agcgcttatg ccagcgaaaa
    22441 atgctgcagc ctttacactc gtgttcctga ctgtggtcaa ttatctgttg tataaccggg
    22501 caattaagcg aggaacaatc atctggggaa aaattgattt cgcttcacag tttgtcctca
    22561 tattcctcgc ctttagtgtg atttggacaa tgggcctcat gggcacggta cgttctttga
    22621 cgcgaaagta ttaccatgtt tataacctcg ttcctgattt tactcctgaa gcgtttacac
    22681 cgaccctggc atactcggct tggtggataa caggggtgac tattgtgttt tacgctgttg
    22741 tgagttttgc cattcttgtc acgctaaaag ctggaagctc gaaaccggcc acatcattgg
    22801 catcctcggt cccggctgaa gctaaatgag aagcttgaaa aaagaggaaa caaatggcta
    22861 accaaagttt tgccaagagt gtcatgaaaa agaccacctt gggggtcatt attggagtgt
    22921 tgttggtagt tggggccaag tttacccatt tcccaccagt ctttcaggtc atgttttttc
    22981 tttatgcgat gctaggggcg ttggtattta ttcttttaga tgctccggca atgaaacggt
    23041 tggaaggagt gaaggctgtt ggggctttag tggtgtttta cctcctgctt tctggacttt
    23101 atattgccgg ggcgtcgatt ctgcctcagt ttgaccctga agatgaaaag gggaaaattg
    23161 aaaaagtctt gaaacttcgt cgcgcccaaa cggaacaagg caaagcggat gagttgattg
    23221 ccagggcaaa ggaattggat gaacgggcta aagctatttc caagcaactg agttctcttg
    23281 gagctgatac caaagttgag gtggtgtcta cgccaggtcc ggcaggtggt ggagcggcct
    23341 caggcgatct tgtggctctt gggaaagatc aatgggaact tcaagagtgc tataactgcc
    23401 ataagctgta tggccagggt gggaaaaagc gtgggccaga gatggataat atcggtaacc
    23461 tcatgacccc tgaacagttg aaggaaaaaa ttatagatcc aaaaagttgg aaagcagaag
    23521 gattcgataa ggattacgat aaagggaaaa tgcctgataa gtataaagat ctgatgttcc
    23581 cacaggaaat tgatgcgctt gttgcctttc ttgcttcgtt aaaagacgaa tcagttaaaa
    23641 ctccgaagcc aataaaaatg aattaatcaa catctgaggt ttttcctggc tatgcaacca
    23701 aaattgacag caaaagcatt gtgtgcgaac aaggaagtcg ggaagatttc taaggttatt
    23761 gttgatccac tttcccacga aattagtcat attatcgttc aggaattgaa tggccacggg
    23821 gcgcaacgcc agattcctat cggccaggtt caagaggtgg tgagtgagga ggaaattgtt
    23881 cttcggtgtt catccgaaga attcggtcaa tttccagtac tggagcgaga tcagtacgtc
    23941 acaattaagg aagtggaaat tgcgcatttg gaggatcatt tacatgttga acctggagaa
    24001 atcttggttc ctttgccccg tttagagcaa ggggtaccaa ggcgcacgtt ttttaccaat
    24061 atgacacatg cgattggagc tttaattgcc ttgccattag tgtttcctgt gttgaagtat
    24121 ctcatgaagc ccatgttcaa accctacgat aatgcctggt tttcggttgg caatgtcaaa
    24181 aaggtgaata aagagaatat tggattccaa ttcaaattta cccgtgggtt taaagaagca
    24241 tttatgccgg agcaacagat tgaaaaaaat atttgggtgg tcaaggcaac tccagcagtt
    24301 caaaaagccg tatatcaggg gaatgatatg aagttttacg ataataaagg ggatgttatt
    24361 tgggtaaaca aatccaattc tccctatatc ggctactccg ggaaatgccc tcatcttggt
    24421 tgtgggtata aatggagaaa gacgaaaaac tttccggacg gggtgttttt atgcccctgc
    24481 catttgagta tttatgatga ggctggaaaa gttatcgatg gcccggcacc gaggccgctc
    24541 gacgttctcc ctctgcaagt tgatgcgggt ggagaagtga aaataatcga tgtggagtat
    24601 aaagccgggg taaataacca aattcggctt ctataaaagc ctaggatgga atggtgaaag
    24661 gcgcatgaag gaagacagtc aaatcgcaag tccacaaaat gcttttgaaa aggttgtgga
    24721 atttgttgat gagcgagtag gcctgaaaac aattcaggcc aaaatgctga atgagccaat
    24781 tcccggtggt tctcgctggg cttacgcttt tggctcggtg ttgttattta tctttattct
    24841 tcaggtcgtc actgggatcc tgctgatgtt ctactatgtc cccagtacgg atcatgctta
    24901 tgcgagtact cagtacatca ttcacgaggt ggactacgga tggtttctgt tgagctacca
    24961 tttttgggga tcctctgcaa tggtggttat ggttttcgcg catatgtctc aagtattttt
    25021 atggggtgcc tacaaaaagc cgcgagaact ggtctggctc gtcggtttgg cgttatttgg
    25081 cattgtgatg ggttttggtt ttacaggtta tcttcttccg tgggatcaac gcgcctactg
    25141 ggcaacggtg gtaggtgtag aaattatgga taaaactcca cttgttggtg attttatagc
    25201 tcgatttctt aagggtggtc ctacgcctgg gcaaatgacc ttgagccggt tctttgtcat
    25261 acatgtcatg gtcttgcctg ctgcgttagc ggggctggct ggccttcata ttttcctgtt
    25321 tagaaaggcc gggcccgcag gtccgttcag aggaactcct gaagagatta aagcaaaaac
    25381 agattatttc tttcctcgtc aaatctggaa agatattgta gctatggcca cggtgttctt
    25441 aatcatttgt tctctggcct tcattgaacc agtggtgctg ctggaaaagg cgactcctga
    25501 tcctggagat taccatcctg agcctgagtg gtatttcttg ttcttgtttc agttgctgcg
    25561 tttgaaaatt tttggcgggg agtttggtca gttccttgga gctatggcca taccaggtgc
    25621 ctttatggcg tttttagcag cgttgccttt tttggatacc aattcagagc gcaatatttt
    25681 taagcgcccc atcgctctgg ttgggtggac tgtgatcatg gtctttattt tggtgtttac
    25741 ggtgtccgca attataaacc gacacttttt agactaattt ctgggaacat atgtcaaata
    25801 cgaagttagg acttcttgtt ttttggccta cattctggac cggatttcca ataaagatgg
    25861 ttgtcgcctt gttgttgctg gcggcacaca tccacccttg ggaggggagc gggctttttt
    25921 tattgctact tgtttccatc ccggttgata tttgggcgct tggtctgtgc gcgagaaccg
    25981 tttttattga ccggctgaag gtcgatccga ggcctggtat tggccttcaa ctttggattc
    26041 gatgggcagt cttcagtgct ttggcattgc cgatccttct tttcgtggtg tccacggtag
    26101 cggaaactgc tcagtcagtg gtttccagta ttgtggaatc tattaaagag aatatttttg
    26161 tcattccggt tgccgagcag ataagtctgg agcttgttat gtgggggagt gtggcgtcgg
    26221 tcgcgctcat tctctgtctt ttcgggtggc tgtttggact agggtggctt gctcagccat
    26281 ttgtaaagga tgcgaagcca gtggtcggtt ccgcagtgga ccaggctaat ttttgggata
    26341 gtttacgtat accagcggat cagcccctgc tcttaacagc atttaccggg ttaggggtgg
    26401 tgttggtgtt tcttttctgg gttcttctgc ccccaacaac ccctcatccc catgaagaat
    26461 atgtctatac gtttgaaaaa aaggaaaagg agatagttga accaaaaaag atactaatgg
    26521 aagcggagca agttctagcc aatgcagagc ttgccgtctt aaagcttcag gaagagaatc
    26581 cattggattc agaatcagct gtaaaatcta aggaccttcc aaaagaaagc aaacagaata
    26641 ataagataga tacacaggtt ccggttaagg gagagggaaa accttagact aagttttgga
    26701 atttctggag tgtagccttg aacaaaataa tgaacgatcg aaggggggcc agaatgaata
    26761 cggccggcca attaggacat acaaccgttt cgcaaggaat gaatagaatg cctggaatgc
    26821 ttaaattggc aagttttttg gcccttctct ttcttccggc tttgggtatg gcggctggag
    26881 gtgctgagat tcaggcaatg tcggtggaat atcgggatgt tccaggaatc ggtagccgta
    26941 atctgatatg gattgtggcc caacagcatt tgttgctggc tggtttcgtt ttgggtgtcc
    27001 caatttttgc ctggatttgt gaacttgttg gttggaaaac gaaagaagct cggtacgaca
    27061 agctcgccaa agaatttaca aagcttttga cgtcagccta tgccaccacg gccctttttg
    27121 gagggatcct tttgttcctc ctgatcggac tttatccaaa gttgatggct tacctgaccg
    27181 atatgttttt tccttctttc ttagtatatt gcctgctctt cttactagaa accgccaccc
    27241 tctacatgta ttggtacggg tgggattaca tgcagggaaa taaaaagaca ttccatcttt
    27301 ttttaggatt cctcctgaat ctttttgctc ttggaatcat ggcggttccc aattcatggg
    27361 caaccttcca agcgagtcct gtcgtgctag gggaaggaga tgcctggtcg agagcttgga
    27421 tggctatgca aaatcctaca tggatgccgg tcaatattca tcgcctcatc gccaatgtag
    27481 tgttaggtgg atttatcgtg ggagcttatg caggcatcag gtatcttttg gcggtttccc
    27541 gtgaagagcg tgagcattac gactggatgg gttatgtcgg taattttatt ggggtgttcg
    27601 gtatgttgcc ccttcccttt gccggctatt ggcttatgcg tgaggtgtac caatacaacc
    27661 aacaaatggg gattaccctt atgggtggtt tcttggcatg gctgtttatt cttcaagcca
    27721 tgctgattgg ggtgctgttc ctgggggcga actattattt ttggatggga attacctata
    27781 gaataccggg gtctgaaaac caatacaaaa aaccggtaat ggcgatgtta atcgtcttat
    27841 tattgtgtct cggaatatgg atgactccgc attctttggt cgccagtttg gctgaagccc
    27901 aaaagatggg tgggactcac catcccttgc ttggtgtttt cggtgtgatg tctgccaaaa
    27961 tgacggtatc gaatatcatg atcctcgtca cgtttatgag ttttattatg tattggcgcg
    28021 cgggaaaaca ggaaacggca ggttgggcaa aagcagctaa agccattatg ggaggattgc
    28081 ttgtcattgc aggaatcgcc gttgtggttc ttggggtatg ggggtatttc gtccctgcca
    28141 tcattcgtat taattacttt tcggtcgcac aggtattaat tgtcctgttc attatgatta
    28201 cgttcactcc gctaaccgcc attttaatga aaagtgccaa aactacaacc gaaatggtgt
    28261 ggggtaaaat gcctattcgg gcagggtata gtttagtttt aaatgcggtc atggtgattt
    28321 tacttatgtc attaatgggg tatgccaggt catcatcccg agtacattgg cacatttatg
    28381 gtgttatgag agatacctcg gaatatgcct attccccagc attagggtat gcagcggcat
    28441 ttatgtctct taacactttt gtgttttgtc taatcgtcgc gtttatcttt tgggtggcaa
    28501 cactaggtga taaagccaag gctcagccgc caaggggatc taccgtggat attcctggcc
    28561 attcggctcc agctatggct ggtggtgcac caagggccgt ggaaaagctt gaataggaat
    28621 ggaataagag aaagactaaa gggccatatt tctttggaaa gcatgggtga ggaaacatga
    28681 gtgaagtcgc gctattacaa ataataggca tgtgtgtcat tggggtagga attcttatcc
    28741 tgctttttat taagggaatg ttccttcggg ttttaggttt tgtcgcgatg gtgttgggag
    28801 tctttagttt aattgccttg agcgttccgc aaatggcctc cttgcctcct gctgtagaaa
    28861 ccttcgatct tgccagcgtt aaaagcccgg atgatttggc ttcaattggg cagaaaatat
    28921 tttttagtaa aggacaatgt gccttgtgcc attcaatcgg gccaagtgag tctgcccggt
    28981 gcccagattt aaatggtatt ggagcaaaac tgtctccgga atttatctat gaaagtttaa
    29041 ctcagcctca agcctatatt tatttggact tcaggcatga tggtgttcca aaagaatatc
    29101 cggcacagat gccccatatc gacaaggatc ctatcggatt aagtaagcag gaaatttatt
    29161 cggtcattgc tttcctccaa aaaatgagtg gtgaaccaat cagtatcaaa gttgaggaca
    29221 ttatggaaac cgagcaaaaa tcggatgatt cgttaaaggt cgcttcggtc tcttcaccac
    29281 tgaaatcgca aatggaaaat tcggcggatc gctaaaggga atagccaggt tgaggagaac
    29341 atcatgaaag gccctattac tagtggagca atcgttcttg ctggtgtgtt tatattttta
    29401 aagtttttac ttccgttatt tacggcacca cttccagcaa gtttaattta cctgtatttg
    29461 gccttagccc tgagcggcat tatgatttat gggaccatga gtggcccatc cttggaggcg
    29521 atgatgggcc cggtcttccg ttttttatct ggtcaaggcc aatcaggaat aggccatatg
    29581 gcaagattgg cagtgttggt aatgttcccg cttctggtgg ggtggcagac gtactccagt
    29641 ttggctccga gcgacctgcc gcctgcggaa aatagaacca ttcatcctgc acctcctggg
    29701 gagtttgttg gtttgtcaaa tccttatcca aaaactcccg aaaacattat gatgggtaaa
    29761 ggcctgtatg cagcctactg ctcaccttgc cacggagcta attttgatgg aaagggcaca
    29821 gcagctcctg ggtttaatcc tcctccagct gattttactg atcctgggac cattgcccag
    29881 ttacaagagg cctatttgtt ctggaggatt aaaaaaggtg gagtaggatt acctgtggaa
    29941 ggaatgcctt ggaagtcagc tatgccaaga tgggaagagg aattgccgga agaatttata
    30001 tggaaaataa taatgggtga atatgacgga gcccatcaat ccccacggac gtgggaagaa
    30061 gaagagtaag cataacccaa tcatgttgtg gataaatgag tggagaatat gacatgtcga
    30121 acggactaag aagcctaggt gccatatcca ttattgggct gttcggagtc ctggggttta
    30181 cggaaacatt tgctagcgaa ccgccggctt ccgatgaact cgggagtgtt tcggttcgaa
    30241 tgtatcaatt agaaggcagc ttaccaaccg ctgaccctaa ttcttcacaa tgggattcag
    30301 tgcctggttc tgaatttaat ctggcgccac aggtgcattg gcctccccga attcaggaag
    30361 taaccgttaa atcagtcaag gctaaagggg ttcataatgg gcaagaactg gctattttag
    30421 tggaatacga tgatcccaca gaagatccgg gggacgccgc tgcacttgaa tttatggttg
    30481 gagacaagcc agcccatttt gcccatggtc aggaaatgct gcaggttgaa ggtggaccgg
    30541 taaacatttg gttttggaaa aaggaaagtg gccaagctgt tgatatgagt gcgaaggggt
    30601 ttaagacctt ggaaactcaa cctcaccagg atgttcaggc aacaggggtt tggcaaaatg
    30661 ggacatggaa agtggtcttt acccgattgt taaaaactga ggacgagcag gatgttcaaa
    30721 tagaacctgg cgaatggaga aatgtggcct ttgctttttg ggatggggcc attgtggatg
    30781 gtcttgtaaa ggaaaaaggt ggacagaaag ccgtttctac ttggtggtat attcgggctg
    30841 aaccgagtgc agataattcc atttttggtt gggttatatt gggcttagcc ctcgcagcgg
    30901 ccttcgaact ggtgattgta aggaaactga gaaaggggca atcagtatga aactgatagc
    30961 gggaggcggt cgtgatataa cagtcatcgg tttggccgtt gtcggattta ccttcacgct
    31021 attttctgga tgcgcaattt ttgaggatga aaaaacctct aagggccgaa agctctataa
    31081 ccattattgt agtcattgtc atggggaaac cggacaacaa ggggaaggct ttaattggga
    31141 gcgcatgtcg gaccccagac ctaaaaatct cgcatccaat gccgaaatgt ccacctttag
    31201 tgatgaagag attttccaca ctatttatcg ggacatgaaa gatacgaccg atcccaaagt
    31261 attagatgac gaggactact ttgccgtccc taccatgccg acatttaagt acaccttatc
    31321 ggaggaagaa atctggtcta ttgtgggtta tgttcggacc cttcatggta tgtcgttgag
    31381 ctatgatttt gaagccagga aaaaagaatt agatgaggca ttccaggcgg cacagcaaga
    31441 gatggaaacg gcaaaagcgt cttttgaggc cgcaaccgag aaagcggagg ccgctcaagc
    31501 tgcttatgaa gaagcgctga cagatgagca gcttgagaat tacgaaccgg aagaagtcac
    31561 gattccggaa gaggaagcgt tcctggcagc gaatgagaaa tatgaaaacg ctaaagcctc
    31621 tgttgataac tttttaaagc gtcctaaaaa ggctcaagtc tctcgaccgg atctctcctt
    31681 atctgaggag gaacgcgccg tccaagctaa tttgggaaaa cagttatata atgataaata
    31741 cggatgtcag ggatgtcata gtctcaacga tgaaggtggg ctggtcggtc cggcattaga
    31801 tcgagcagga tttcgcctca atcctacatg ggtctatcgg tggattctgt atcctcaagc
    31861 catgaaaaaa cataccagga tgccaaattt agggatttca gaccaagatg ccaaggcaat
    31921 cacgtattat ttagggacac tccaggcccc aaaaccagaa aatccagttc ccctctcaga
    31981 ataaaaacaa aacggtcctt ctttccacga gacgcgtgcc tttaggcttg cattagccaa
    32041 aaaaggtgtt cacctacgat ttatccatgc taaggacctg gtttgaatct ctaaggaatt
    32101 gtgggaatgc ttttgataaa caggctagga taaaaaataa aaaaagccta tgagtggctg
    32161 gaattctcct gagtgattgg ccgattaaaa taaaactaat cggtttgttt gtagtcctta
    32221 cttattttgt atatttatgg atgccccatc catattcctc ctaaaataag aaccccaatc
    32281 cagacatgtt gtttgaataa aaagaaggct ttttcccctg aaatatttga gcggagaaga
    32341 agaacttgat agcctaataa cccagctact cccattaagg tcatagaata ttcaatgcca
    32401 agatgattga tgttggcgac cagagacaga catgccagca tgaccaaccc cgcaatgccc
    32461 accccaagcc aggtgtagga tccaaagaga atggcggtgg atttgactcc aatcctttgg
    32521 tcatcatccc tgtcctgtat ggcgtaaatc gtatcgtagg ccatggccca acacaccgtt
    32581 gctgcgaaaa ggatccaagc cgacaggtcc aattcgtttc gaacagcggc ccacgccatg
    32641 atgccacccc acccaaatgc taatcccaaa acaagttggg ggatatggat gaaacgcttg
    32701 cagaaaggat aaatcccagc aagcagcaat gccacaggac tcagcaaaag tgttagagga
    32761 ttcaagagcc ataacaaaat ggcggctaac ccaagcaaaa ctgccagaag tgctaagcct
    32821 tgggaaggcg ttaattggcc agaagcaagg ggacgatgcc gtgttcgttg aacttcccga
    32881 tcaaatgacc ggtcagccag gtcattcata atcacccctg cacttcgcat gagaaatgat
    32941 ccaaaaatga acaccaaaag gagcttaaga ccgggccaac cctcggaagc aagaaacaga
    33001 gaccaaaggg tggggaagag taaaagcaga gtccctgttt gattgaacaa ccggatgaga
    33061 ttcgcaaatg caaggaaagg aggcatggac gctatgtaga gctgcctaaa acaataaggg
    33121 acacggatgg taagacatga ccattggaaa agacgccatt atcggaacac ataaactatg
    33181 agttttgagt catgcgaatg tgaatattat tgctcaggaa gcccccccaa tgttatttca
    33241 ccataaatcg tgcgatacag aggattcgat ccggttcatt cgaaggagca accacgatca
    33301 tccattcccc ggtgtgtaaa tccggatgta gtgatttcat gctccacatg cgatagcctg
    33361 gagaaggttg aatggggatg ttcacttctg aaattttggt ccaagttcca tcggttttgt
    33421 gatgccagct gtgacgaatt tttccttttg tggttgcttc gatttttgtc caaaatatca
    33481 cttgcgaagt ttgtgatgtt tggatcattg gaattgccgc ttgcccattt ttatcttttt
    33541 cgcaataggc tggaggacgg aatattccct gtgggttcct cttgtccact tctttagata
    33601 aaacaacggt cttaacaaga aagtcagcct gggctaacag aggtgttcct gctaggccga
    33661 tcaaaagccc gattcccctc aatatgtgtt tcactcgatc aaccaccttc agtcctcatt
    33721 tggatcgtga gacaacgcca acctaagaaa ctaaaatata cctgcggtct tgtcaatgga
    33781 gaaggtgaag ggtgggcata agtgcggaac ctacttattc ctcagcaaat tccctccact
    33841 ttttgataag acccacgacc ttttgaagtt gacgttacta agcgacgaaa ggtataaacg
    33901 accacaaggt agcctaccaa actcgttctc cagtaagggc ggtccttcgc tatttgattc
    33961 cttccaatgt gagccggcgt agctcagtgg tagagcagct gattcgtaat cagcaggtcg
    34021 gtggttcaat cccactcgcc ggctccagtc aatatccaat cggacaagtt tttaaagtaa
    34081 tagcccggaa ggattccggt tattttcttt cacagcgaat gttggaaatt tgatctgaaa
    34141 aaatggagct tgtgtggcat cggtatgtgt atttgggttc agggaagcta ccttgatagt
    34201 tcttacgtag tggacctgaa ccgccaggtc cttttacttt tctgtcttga ggccacacaa
    34261 atgactgggc cgaaatgttc attctcccct gggagattcg aatcttggta ctttccattg
    34321 ggtgtttctt gatcaacaat ccctctgcag aattttgaat gtaattaggt cgttttccct
    34381 tcctgccaat ttcattttgt tgcgaatctc aattggtcgc catttccctc accttcctct
    34441 caaattcacc cccttgtcag agaaaacatt cgggtccctt atccaattgg gatgtggtgg
    34501 gtcttgtcag gatgcttaac gggtttgccg ccatacaact ataaccaacc tgctctcaaa
    34561 tcacatgatc ttgccctact gtccaagggc gcctagagac atctgtcaac ggttttaaca
    34621 tcggaatttt gttgatcaag attgtggtgg tgttccaacc cttcaatgac cccctgttct
    34681 tttagatttt atgcaaatcg gggatgttga ttaaattccg aagagaggca catggtttgc
    34741 ttaaacgaac ggaagacagg gacacttatt atttgaggga cattatgaaa atctgtgaaa
    34801 aatgtcaggg tgcaatggtg gtcgagcgcg ctgtggattt ggaggtcgga ttaagtcttt
    34861 tgtattttgc ttgcttaaat tgtgggaaac ggattcaggc ggaaaaagaa ccgagacctc
    34921 tagtccatta atccttgatt aaggcaacca tgaatgggga ggagttatgc aagaacagag
    34981 tcgaccattg acttatgatg aacgaaaggc ggccgaagca gcttttcgag gggcgccgtt
    35041 cgatccacaa ttgtccgaat cagccagaaa ggtttatctc gggatttcct ccgcaatggc
    35101 gaacaaacgg aatgaggcgt ttcaggaata tgatctttcg caaaggccgg tttcaggaaa
    35161 aagagcgtca aatgaatcgc agggtcgatc gagagcaaaa tcttctttat ggtaatctgg
    35221 gctcactttt ctgaggacga cccgaggatc gggtcgtcct cagttagcac cttccattat
    35281 gcttgaccgc ccggtattcc tcattccaag tatttttttt tggttacggg atcgccccgg
    35341 cgaggagttt tgtcggagag agtatatctg ggttgtgaag ttaactatgc caaagaatgt
    35401 tcgggtgtct tcgaagtctt gagtcaattc tttccgccaa ttttggtgct gaggtatgat
    35461 gatcaaaata ccatcaccat attcccgcca ttgatgaagt ggtcaattaa caaaattttt
    35521 aactgcaaca ccacaaattc cttttcaccg gatcaatttc attatacacc tctccctttg
    35581 acatacagag atggccccca atttttggac agctccctaa gtgaaatctt atgcattata
    35641 ttttcgttca atgaatgggg cagaagatga caggaggctc tcgagagaat cttttcccct
    35701 ggctgagtga acggagcgct tggtccttgg gttcgatggc tgactaaagt agcggaaaag
    35761 cattaacccc aaatacctaa aaattccatt ttaggtttac gcatagctct tttaggttag
    35821 ggaaaaggcc ttccaatggt gtctggtttg gaaggttatg tcgcaacaaa ttttatgcct
    35881 atttgagcta gcagtacggg ctcatcttga tcaatcctat ttttggtttt gggtgtctct
    35941 atggcgggat gaagatgagg tgaataggga cctcaggccc ttcagtgctc gttattgagg
    36001 aagttgtgct tttatttttg ccaatgccaa ttgttttagg gtgcgcaagt ccaacttacc
    36061 tgttcccaat acagggagac gatccacttt gatgaaattg ctccgtttgg ggatgaaaag
    36121 attaggcaac ccatgagtgg tgaccttttc caaaatttcc gggatggccg attcatcgag
    36181 ggtatgtaag accaccagtt gctccccttt ccgttcatca ggcacggcgg tgacagccaa
    36241 agtcatagtc tcggtatttg ctgcttccat tagggcactc tcaactcgtc catgaggaat
    36301 catctcacca ccgattttgg aaaatcggga gaggcgatcg gtaatggtaa taaacccatc
    36361 ctcatccagg gcggcaatat ctccggtaat gtaccatccc tgtcgtatca cttttgcggt
    36421 caaatcttca cgtcctaaat acccactcat gacgtttggt cccttgacta acagcatgcc
    36481 tggagtcccg accggcaatg gttgctcggt gtcaggatcc acaattctga cagacacgcc
    36541 tggaagtggt tgtccgaccg ttccccgccg agaggcggat tggaaaaatc cagaagcccg
    36601 aaaatccgga caattgacgg caatcaccgg agcacattct gtgactccat atccttcaat
    36661 gggacgaatg ccaaatcgtt cttcgaatgc gatcagtagc cggtcgggta atttttctgc
    36721 gcctgtgagc acgatccgaa gggaaccaaa ttgttcaggg gtgcagcggc gcaggtacag
    36781 ttgcaggaaa gtcggggtcg ttaacaggat ggtgactcga tgcttgtgga tcagattccc
    36841 aatgggaccg gcatccattg gagaaggatg atagattacg cccgctccat gaataaccgg
    36901 aaaccacagc gtggtcagat agccgaaaga atgaaagaac ggcaaaatcc ccaaaatccg
    36961 gtcgttgtga tccaggtgaa ggacttgggc aattccttcc acgtttgagt caaggttgaa
    37021 atgactcagc gggacgcctt tgggctctcc agtacttcca ctactaaaaa taatcgtggc
    37081 gatatcatca atggatggat gcatggtcgc cccacaaaaa cgctccacct tccggattgg
    37141 agcgaagagc ccaaataaca tagcggtaat tcgagcctgc ccatcaatcg tgtttcgaat
    37201 ctcctcgatc cagataggcg tgaggttcat cggcaattcg agattggcct tttctaagaa
    37261 aacgcgactg gtgagaacag tcactaggcc tgcttggttt gatgaagatt ccatcccttg
    37321 caccccaacc gtataattca agttgacggt ggttcttcca gaaagcgttg ccgccatatt
    37381 ggcgagcgcg ccacctacac ttggaggtag gagaattccc accgtttgtt gcccttccca
    37441 gtgagatctg agggctcttg ccaaagcgat agcgcctgtc agtgcctcga agcaggagag
    37501 tcgaggtctg gttgcatctc caaatacgac gcgaaacggg tgtttgcgca tggaccagac
    37561 aaagctgtga tggagagggc gtcgtgtgag ttttcgtaaa tgccatgccg tttctcctaa
    37621 ttcctggacg gcctgccgta cctcttcagc ggatgtggtg gaggggaggg gggcgccgac
    37681 agagagcgta attggatagg ggaatcgggt tggccatttt gctaaaaaac gtccgccgat
    37741 gaagctaaag atactccccc agacccggtc aaggttaatg ggaataatgg gcacatcccg
    37801 gcctttgaca atacgagtga atcccgaacg aaagggtaaa aggttgcctg tgcgggtaat
    37861 ttgtccttct ggaaaaatgc aaaccagttc tccttgatca agactctgtc ccgcttgtcg
    37921 tatcgcgtgt aagatttctc taggggatcc gttcgaagaa attggaatga cccccatgat
    37981 tttggcaaag ggttggaaca cgcgatggtc ataataattt tggtcgacga gaaagcgaat
    38041 ggggcgatcg gtggtggaga gtaggagtag tccatcgatg aaggagacat ggttgggtac
    38101 cagcagtgcc ccaccttcct gcgggatatg atcccgtcct gtgatggtaa gtcgatagat
    38161 ggtgtgagta aacaagacta acaacaaccg aataaacatt tccgggagga cccgaagtgc
    38221 ccaaatggtt agagctgcgc ttcccagacc ggatatgaga aaaatattgc tggcgctgag
    38281 tccgatcttt gagaagaaac ctgatcccag agaccccaac aggactcctc caaagaccag
    38341 ggtattggca aaggcaatca cggcgcctcg tcggtcggct ggggatcgcc attgaatcag
    38401 agcattgagc gggaccacca caaatccgct ggcgaggcct agacatccca tggcgatgag
    38461 ggttcctcct aactggggct ccccaaaccc taaggcgaaa agactggcgg tgatcccagt
    38521 gcctcccaag ggaaggtagc ctaattcgac tttggccgct gagagttttc caaccaatag
    38581 ggacccaatt ccgactccga ttccgaaggc tgccagggga aggcctgaaa gggaatctga
    38641 caggtgaagg acgactttgg cgtagatcaa cacatcctgg ccaactaaac tggccaaagt
    38701 ccaaaatgca atggccccca tgatgcccaa tttcaagact cgatcgaatc gaagagcttc
    38761 aatggcggcc ttccacgttt ctcgaactcc cccatcaagt cgagcacgtg gaactgtggg
    38821 aataaataat gacgcccata atcctattcc tgagcacatc atgaggacta atcctgtcaa
    38881 ccatggggtt tgaccggaaa ggtcaagcat gagacctgcc aaggcggttc ctgcaatgat
    38941 ggcgatgaag gtccacatct cgatttgacc gtttccccag gacaagcgat ggtgaggcaa
    39001 gagttcagga ataattccat atttggaagg actgaaaagt gcactttgtg cacccattgc
    39061 tgccagcacc aggaggggaa gaattccacc cagaggattc caaaagagag ctgcggttcc
    39121 ggctcccatc aataagactt ccacggcctt catgacgacg atgacggttc tcttgctaaa
    39181 ccgatcggaa aagactccgg caaaggcgga gaccaccatc aggggaaggg taaagatgac
    39241 aaaggcttgc gtggttgtcg cctgtgtggc ggcttcgagc tcagggccag aagcacctcc
    39301 tgccgaggcg gccacttgct tgatagccag gagggcgaca atgagtttcc aggcattatc
    39361 gttgaaggct ccaaaaaatt gggcgacgag cagtccgcgg agagccgagg attgagttgc
    39421 gggtgggggg gaggctgagt aggcttcagt caccgggatc acgaggtatg agtattgggt
    39481 ttttcttctt tttctcttgg ggtccaggag atattaaaca aatacggcgc ataacgaaat
    39541 cacaattttt tgttttgttg ggttatcttg gagaagccat acttacccgt caatcgtttt
    39601 tatccatatt tttgattttt ggtacggaat tagcgacgtt atggattgat ccatgtatgt
    39661 cgggacgagg cggattatca aaacaaagaa atcacactag tatctgtttg tatcgtcaat
    39721 ccataccttc caagaagtcc tccatcagtt taagctgatt cggaattccg ctaccctcct
    39781 gggagtgctt taaatgggaa cgatgccaca ttttcttcgt ctgccacggt tagccttttc
    39841 cctttgacaa ctttgacaat ccggttgttc ccatggtgca ggagcatgcc tgttctgttc
    39901 ccaaaactct taaggttctt tcccatatag ccgatttatt tccgtatgcc acgccaggga
    39961 tttggtggag atgttttggt gagttcttta ccgttggaat aacccagcct ccggcaaaga
    40021 atttagaact acgcgatatc acaaaataag ttgcaacgcc acctcggtag agggatttcg
    40081 acggatattt tcgcgatgac gactcgtacg gaaacatcag gattttcgga aggccacatg
    40141 gaagcagtgg gggaatccat cttccataga atgcgaaacg tcctgaatag catccatgtg
    40201 tctgccggac ttattcacca tcggcttcgg gaatctcatg tggctgatgt tgggcgggtt
    40261 gcggatatgc tcgaggccca tagtcatgat tacgggtggt atctcactca ggatcccaaa
    40321 gggaaaaaga ttccgaattt cttggggcaa ttgaatgagg aattgtcaca gaaccatctc
    40381 gataccctga ccgaattgac cacgctcaat tatcacctgg agcaattgga atattatttg
    40441 acggttggcc aaacgcccca tcgcgtgggg ggatttaaag ataccattca gtttgcagcg
    40501 attatggatg aggctttggc atcccatcag tgtgaattgg atcggatggg ggtccaagtg
    40561 tgtcgggagt accaaagggt tggcgaaggt attctggaag tatccaagct tctccgcatt
    40621 ctggtgaatt taatccggaa tgcgatcaat gggatgaggg agataactag ttggactcat
    40681 cgcctcactc tttatgtctt tccctgtccg gaccgagaag ggtttgttcg tttgcaggtt
    40741 gctgatactg gaaggggaat tcctttagaa ggtctgactc gggtgttttc tccacaggct
    40801 tctggggagc aaacagacct gttaccgaat cttcatgcta gtgctctagc ggccaaagag
    40861 cttactgggt cactgcgggt gtggagtgat ggtctgatgc agggggcaat atttactctt
    40921 gatttgccag ttattcacat ggagggagaa cggtaatgga gaaggaacaa gccaaccggc
    40981 gtattttggt gattgatgat aatgtggcga tccacgaaga ttttcgaaaa attcttgagc
    41041 gatcattaga cacctcgctg ctgcaagaag ttcgggcgga attgtttgat gatgtggaag
    41101 aacaaaaaat tgttgaaaag tttgaagttg aaactgcgga ccaagggcaa atggggcatc
    41161 ggatggtgca agatgcgatg aaggctgaac ggccgtatgc ggttgccttt gtggatatgc
    41221 gtatgccccc aggttgggat ggagtagaga cggtggagca tctgtggcaa gaagatcctg
    41281 atttgcaggt cgtgatctgt acggcttttt cggaccatgc gtgggaagat gtcatccaac
    41341 gattgaataa aaatggccaa ctcctcatct tgcgaaagcc gtttgataat attgaagtgt
    41401 ggcagttggc caattccctt accaagcgtt gggcggaatc gtgtcaagca aagtcacagt
    41461 tagaactatt ggccaaatgg gcagaagaac gagcagaaga aaccataaaa tccttttgac
    41521 gccagggaat ctaggttcgg ccgtttggga cctttccatt taaaagggta tttctggaga
    41581 agatagggga ctcccgttac tgggtgcgca atttgtcgtc atgccaggga atcctcaact
    41641 caggggattc tgtgaatgag atgccagcgg tgttcaagac gaaacttcat ttatccaccg
    41701 tgtgatgctg ttttgcaatt tcatgagttg tttatcgttc aaaattgatt ttctctcctt
    41761 accttcttca tggaatggtt catgtcatgt cggaatctaa tgggcaggtg attccgaaat
    41821 ttccgctgct ccgtgtggtg atggtgggaa ttcaggaaag tgacctttcc ccatcctttc
    41881 atcgcgtgtt ggcttctcag ggccccatac ttcaagtcac ttggcttgat tatgcctcaa
    41941 tgcacccttc ctgttttgat tcccacaacc catttgatct cattctcgtt gactgtcggg
    42001 taaaccggag ggggagggat attgcttgcg atatcaaaaa aatctggggc aatgcgttca
    42061 ttttgggatt gattgatgga gagagttccg ctgttgagct ggaagacgac tcggctatcg
    42121 attggctggt taatgaaaaa atggagggta tcgcgatgcc ctgggccatt cgagaggggt
    42181 tggttcgacg tcggggtttg atcgaacgtg agcatttgcg caggcaggtt gaaggtgcct
    42241 catatggtaa tgagatggct gatgttgcct caaccgtatt gcacaatgtg ggcaatgtgc
    42301 tcaatagcat taatgtggcc gtgcatgtgg ttcatgagtt aatgaatcag tcttcgataa
    42361 ttctggttca tcgaatcgcg gaactgttaa agaatcatga ggagaattgg gaaatctatc
    42421 tgactcagga tccaaaaggg aaacggattc ctccggcgat tatgaaattg gggagccacc
    42481 tcatagcaga gcaacagacc gtgctaaaag aattggaagg cctggttcgc aatattgatc
    42541 atgtcaaaca aattatcatt tcacatcagt cgatggcgaa gtctcggggt atcatcgagg
    42601 ctctctctgt tgtggaactt ctgaatcaag ccgtggagtt aagttttcac gctggagatg
    42661 cgaaatggat caggatcaaa cgagactatc agtctgtgcc gcctgtcgtg accgatcggc
    42721 atcagttgct tcaaattctg gtgaatctgc tgcgaaatgc taaacaggcg atgcaattac
    42781 aggtcggagt tgatcatctg ctcaccatac gagtagaagg tcgcacggat gatgggttgt
    42841 cggttgtcat cgcaattcaa gataccggca ttggaattgc gcctgaacat ttggccagaa
    42901 tgtttactcg cggttttacg accaagcatg atggcaatgg gattggtctg catagttcga
    42961 tggttacgat tcatcgtatg ggtgggatgt taaaggccca tagtgatggg attggcaccg
    43021 gcgcgacctt gacactaatt cttccggttg aggcaggggc aggacaggca tgaatcccgt
    43081 ctgtactcta aatcgacgga tcttagtgat agatgatagc ccggacattt tgcaggattt
    43141 ccggaagatt ttgtgttccg caattagcct catttccaat gcgtggcttg aaagcagcca
    43201 ggatttcttt gacgggccct tcctgcatca tggcgaagaa cccttcgatc tccattgtgt
    43261 gaacaatggg gagtcagggc gtgatgtggt cgcgcaggct aaggaacggg gttcgccctt
    43321 tgcggtggtg ttcctggatg tgcaattgcc caatggttgg gacgggattg aaacggttga
    43381 gcgaatttgg gatgaggatt ctgacgtaca ggtggtgttg tgtacggcct atcccgattt
    43441 tggttggtcg gacgtattgc ctcgattagg acatcgggac cagttattaa ttctacgaaa
    43501 gccctttgat ccgattgagg tatggcaatt gtctacggct ttgacaatga aatggcattg
    43561 tgcccagcag gcccggttgc gggttcagga acttgaacag accgtcatga ctcgcacggg
    43621 ccttcttgaa gagaccaacc gttgtttgga gcaggatttg ctcaggcggc aagaggtaga
    43681 agtggagtta gctcaagtgg tttgtgatgt ccaagaaaga aatctcgaac tttctgctat
    43741 gcgggaccaa gcattgaatg aaatccatga acgggaacga atcgaacgga ttttacgtca
    43801 ccaaaccgaa gaattggccc ggtcgaatcg ggacttggag caatttgcat cgcttgcagc
    43861 tcatgattta caggagcccc ttcattcaat ccaggtattt cttgatctct tacaggtgaa
    43921 atatgggtca gccctcacca gccaaggtct gggatatgtt gaccgagtca aaaacgcggc
    43981 ggatcgcatg caacagctaa ttcagagcct gttagtgtat tccaggatcg atttgccgca
    44041 cacctcaggg gagccgcttg tcttgcgaga aatggtcgac gaaatcctgt cggatctcgg
    44101 cgcgcggatc gaggaaatgc aggcggtcgt gcagattggg gaattaccta ctatttatgg
    44161 aaatgcgttt caagtccgac aattgttaca aaacctgctc ggcaacgcct tgaaatttca
    44221 tcggccgggg gttccaccgg tcattcgtat caccgggacg attattcaaa atcgacgtca
    44281 caccggttta ggccaacctg gatggctttg ccaaattgaa attcatgacc agggcatagg
    44341 tattccatca gaacagttcg ggaaaatttt tgggatgttc aaaaggctgc atcggaaaga
    44401 tgaattcgaa gggacaggga ttggtttggc cgtatgtcaa cgtattgtcg agaagtgtgg
    44461 tggtgccatt tccgttcgtt ccaagttggg agaagggtct acctttatag ttacgctgcc
    44521 aattcagtaa tggggagaac tgatggccgg tttaaaagca ttcgattttg gttcataggg
    44581 aatacgacct tttcaaatat ataattagtg aagctcaata ttttatggtt cagattcctg
    44641 cgcatcgtcg tgtattagtg gtcggggctc atcccgaaac ccaaatcatg ttgcgcgatg
    44701 cgttgtctgc gcccgctgtg ggtgacatgt cagtagggag ttccttaaat ggcaaaaatt
    44761 tggcaacctc tggtattcag ccgtttttaa ctcagaccca gttcgctctt gtcagcgtgg
    44821 aaatgggcct ccatgttttg tcgaagttcg cggaagctca agttactggc aatccttttg
    44881 ttcttgtcct tattgatggg cgttcagagg attgggaggt cgttcgagac ctggtggagt
    44941 cgctttggga atatgatcag actttacggt gtatcttctg tcttccccct gatcgggcat
    45001 cctggcccca tcgtctggtt gtgagccgtc cagagcaatg ggcggtactt cggagccctt
    45061 ttcttgaaga agaagtcttt caactagcca gttgtttgag tctgccgtac ccggtatccg
    45121 gtgaaactgt gctcaatggg gcaacctatt caggggaaaa tggctgtcat tccaaatctc
    45181 cttcggatct ccatgtccat aatggtgaag cggcgacggg agcattggaa tccgctcgtg
    45241 gtgaattggc tgcttccaga tattatgttg ataatatcct ccgctccatg gcggattctc
    45301 tgctagtcat taacgcagat atgactattg gggcggtgaa tccttccttg ctaaatcttt
    45361 taggctatca ggaggatgaa ctcatcgggg agtcgcccgg tttgattttt ggcgaagaat
    45421 tttcccaagg tgcgatcatc gaaaacctct tacttcaagg atcggtgagt ggcgtggaat
    45481 ccagtttcct gactcatgag ggtcagaaaa tcaccatttc agtctctggt tcgatgatgc
    45541 aggacctgca gggacaattt caggggttgg tgtgtgttgc ccaggatatt accgaacgaa
    45601 agcgaatgga agaagagaag ctgcagttga atgagcggtt aatggaaacg tcaaggcagt
    45661 tgggaatggc cgaagtagcg acaagtgtcc tgcataacgt gggcaatgtg ttaaatagca
    45721 ttaatgtatc tatcggagtc atcacggatc ttttgaaaaa ttcgatggtc gaggatgtgg
    45781 gtcgaatttc acagttattg gacaagcatc atgaagatct aggaacctac ttatcgcaga
    45841 atcccaaggg gaaacaagtc cccggttatc tggagaaatt atcggggcaa ttgaaggagg
    45901 aaaagcgagt ggctctattg gaattagatc gattgcggga gtatgccggg catgctcagc
    45961 aatgtgttgc gacccaacag gatctggcca agccaagcgg gatgacggaa ccggtatgtg
    46021 tggctgaggt ggtagccgag gccctggcag ttaaccaaga gatgttggag gaaaccaaaa
    46081 ttacggtcat tcaggagttt caggaagtgc cacagctcat cgtggataag catcaactcc
    46141 ttcaaatttt ggtggatgtc attcgcaatg cctgtcaggc catggagtcg gttgcccgga
    46201 gagacttagt tgttcgtgtc aagctcgtca ttggtcctcc ggattctatt tgcctggaag
    46261 ttcaggatac cggcagcggg attcctcccg acgaccttac gaaagttttt gggcaagggt
    46321 atagcaccaa atatggagga cggggcttga gtctgcatca tggagcgttg atggcaaaaa
    46381 atatgtgtgg cgcacttcgc gcccacagtg agggcctagg acaaggcgcc acgttttttc
    46441 tagacctgcc tgggaatttt cattttcccg atttgtaatt tctccctcca gccaattcaa
    46501 tcattctttc tgcgcgcctc tttatgagtg ttgcgttgta ctgttgtttc tttctattga
    46561 ttagcatttt tcatgtcctg tctcacgggt ttgcacctca agagtcagtt tggtgaagcc
    46621 gagaaaaatt gaaggagtaa gaggacgctt tccggcttcg ataatccttc tgtgtttctt
    46681 ggagtatcgc atgtgactga atattggcct cgaacagagg atgactcttg ggatggggat
    46741 ggagaaagga cccacgagag ctggggggct ttgtcttatg gtggggagtc actggcgcct
    46801 tggtcaggat cattttcacc cagattgttg atacgagggc atacaagttt tgatgagctt
    46861 caagcccaaa agttatctca ttttgaccgt gaggtgaaag tgcatgtgga ggctgtttcg
    46921 ggtgatgcag gaatgatccc ggttgatctg gagcgcgctt tagaatttgg ggtcgaagtg
    46981 tttctcctat ccgagggatg cgcagtagag tcttcgcgta ttgtaagttt gtggaggcat
    47041 agcctcagaa cgggatacct tgctgcgttg attgccttga atcaggggtc gaatcaacgc
    47101 atggtgtggc aatcgtttgt gggaggggtg cttcatgata ttggcatgct cgtgtttttg
    47161 actcagcagc cggagatgtt cacgaccgtc gtagagatag cccaatgccg aggagggaac
    47221 ttggccgtgc tggaaagaca ggtcttgggg tacacacacg gggaaagtgg agtcactttt
    47281 ctggcacgat ggggtataga ggatatgtta cttgcgattg tggcgttcca tgatgatcca
    47341 tcgaaggttc cccatacgac cttctgtcca ttgacggctg tgtatgccgc aaattttgtt
    47401 gaggggggag ggatcgctca ggatggggat ggagtcattg ggaaagatgg agaagagtat
    47461 ttaacccggt taggtctgtg ggatgatcta ccgatttggc aaagctggat gccaaatatt
    47521 cagcagatgg cgattcatta aatacgagtg gcactcaatt cattattcaa cggtgtgcat
    47581 gttcactaat cggcctccct tgtttttatt cagatgcttc tccttacttt ctttagcatt
    47641 tgtctagata tttttcctca tttgtgtttt tagcctggtc tgaaattcct gaattctttg
    47701 ggttcccatt gtcaggaaga gggtgtagga tttgtctgaa gggtgcgtct ctgaattata
    47761 gaaatgcggg ggggaatgag ggcaggtcct tggacgactc gtatattgtc acactcttga
    47821 ttggaatttt ctggcactct ggtgattgta tgcataacga atactgggta agctggtaga
    47881 taatgatgtt gaggaggggc atgaggattc tgtctagaca cttaattggc ggagtaatcg
    47941 ttggggcatg catcctgttc ctccctcaga ccgtcgtctc cttggaccct ccttcggctc
    48001 cctgtcaaaa ttctgaagga tgtgttacga aatttcttga acagctagaa tcatttggag
    48061 ctgattctga agccggatgg gatcatgcaa agtctttcaa tcagattgtt acatctaatg
    48121 ataattccgc tttggtcaaa cgtgtcgggt tccgttatgg atattggctc aaggaatcct
    48181 cccctcttga agctattccc ctgttgaaat ccgctctcca tgatttccct attctgggcg
    48241 attatcttac cttttggatg ggacaggcat atgcttatgc tggtttaggg agggaggcgg
    48301 cagaggcgtt tcaacagttt aatgagcatt atgccgattc tttgcttcgc gctgaagcac
    48361 tctatgcggg tggggatgtt ttggcaaaat tgggggattg tgacgcagcc ctctcgatgt
    48421 ggtcccaggc tctcagcata aaatctgacc atcccaaatc cgcagaagcc ttcttccaaa
    48481 tgggtttatg ttctgctcaa ataggacaac gggaaaaggc gattgagatt ttccgtgaaa
    48541 tatggtggaa attcccactc gcccctgagc ggctgaaggc tgaatcgtgg ctacgccggg
    48601 aagtgggttc tttgtttttg ccctcaccgg aagagcgata caagcgtagc atggcattgt
    48661 ataacggggg agctttggag gaagccgttc aggaatttca aaccattacg actctgtctt
    48721 ctcctattcc tcagtttttt caggtccaat acaccttggc catggccttg gttcgtttga
    48781 aacggtatga ccaggcagaa gcgatactga cctcgctatc ccgttcctcc tcctccagaa
    48841 cagatgatgc atgggtatgg ttgggaagag tgtatttgcg tcaggggaaa ggggcagaat
    48901 tggaggctct tgtcgaaacc tccgcaatgg gtagggtaac cggtgatcag caatcgctgc
    48961 tcttcacctt ttacggtatt tggttggaag atcatgctcg ctggctcgag gccgggaagg
    49021 cctataggcg ggcaggcgct gtggctcgca cgttaacgca gcggttggat gcgctttggc
    49081 gagtggggtg gattcactat caacagaaac aatttattga tgctattgag atatttcagg
    49141 agattaccca ggcagtcgga actcctcaaa ccgaatcttc tctccatgcc gcctctcagg
    49201 cttcctattg gttggcccgt gcccaggaac atcttggaca gatggaggcc gcgcgagaac
    49261 gtctaaaaaa attaagccag gactatccat ttacctacta tgggcaattg gcagatattc
    49321 gactcggtcc ctcagaattc tcgacgaaac aatgggcggt gttggcatcg actgacgtga
    49381 tgcccgtgga tactccaact caccttcagc aggatatcca ctatcaaaaa attcaagagt
    49441 tacgaactct tcgcctgcat aaggaggcag tcaatgagct tgaggtcgtt tctgcccgct
    49501 tcggagccga ccccaaagct ttttctcaat tggtttcatt ggctagtgaa gttggggcct
    49561 acgatgtcgg gattcgatta tctattcgtc attttgggtc gacgttgcgg aagggacagc
    49621 tccatgcctc ttctgctgcc tggttggggg cttttcccat gggttaccaa agggttattc
    49681 aatcgttcgc cccaaagcaa gtggatccgt ttttagtcgc ggggctaatt cgcgaagaaa
    49741 gcctatatag tgcccgcgtc gtttccccgg ttggcgcgat tggcttgatg caactcatgc
    49801 cagagacagc caaaagggtt ggccgtcagt tggggttacc ggcctccgat tctgatcgga
    49861 agggcctggt tgaaccaaac agcaacattc aattggggac gtattacttg gggcagttgc
    49921 tgaatgaatt tcaaggcaat atcatttatt cggtggctgc ctataatgct ggcccacagg
    49981 cggttaaacg gtggattggg caaaatggtc atcgagatcc ggatgaattt atcgagctga
    50041 ttggatatcg agaaacccgg ggatatgtga agcgcgtact tggaagctat cgaatttatc
    50101 gaactctgtt tggggatgtc tgccggggtg tttctcttga caggttttgt tgaacgaatt
    50161 atagtccgcc tcgtttgccc tagctaataa ggaggcgtgc acgtgagcgt agataaatta
    50221 gaaacgttgg aggttcgagt taagaaatta cttgatttgg ttattgagct acggcaggat
    50281 aaatcccacc tggaacagga attggaatcc actcgagagc ggctggccaa acacgaagaa
    50341 atgtctgaag gatgggaaga ggagcggacc acgatccggt ctagaattga aaaagtactt
    50401 ggcgagcttg aatttttgga ccgttcgaac gattaaccta tgggattagg gaatgacgag
    50461 gaccattgaa gttgaaatct ttggtcatcg aatctctctt caaggagagg gagatgaggc
    50521 gtactttcat gagttagctg ggtatgtgga tgcgcaaatg cgaaaccttg ctcaaaaaac
    50581 ccgaacaagc actcccacta agctagctat tttggccgcc attaacatta cggaccagct
    50641 ctttcggcaa gagaggcacc gacagtttgg agaagctgaa attgaacgac ggactcaagt
    50701 tttattggaa acgatagaaa agcacctcga gactaacccg aattaaccat tttcctcttc
    50761 gtatcctctc ttcctgccat agttaggtat tacctttgaa atcaattggt tgaattgcat
    50821 tgttctgctc gttgccgaat ccttgtcggt ctattcccat cattcaactg cctttttatt
    50881 gaaatacctc atcgtcttct gatattttac tcctcataag ttattgaatt gcaaagaaag
    50941 aaaggaatat ccctcttgta gggggaatta aactggaggc ccactatgac aagaattttt
    51001 atagtcattg tgggtttttt gtatgatggg attgtacgaa gtaggaattt tttatttgag
    51061 ggcaggttaa aatcttggaa atcgtttttg aagaggcaaa ttgctcattt ctattcgaat
    51121 ggctcgcctt gtgatctaaa aattttaaat ttttggcctg tgtggtgtcc atgtccttcc
    51181 aattcaatta gtagctgtga aggctggatt cattgggtga gtccattgcg tggtgctatt
    51241 gggccttttg gcttagagta ttgccctgtc tctcctcctt ctcgttccct gcaaatccgg
    51301 gccttctgtc caatcagata ggcttttctc cagttttcgt tttccttcct tttctggttt
    51361 tcccctgtgg tttggtggga ttatttccga ctgactgggg gaaaataaat cttatctgat
    51421 gatatgaaga aacgaatagg agctaaccta tggacacggt aattcccata cttgcactgg
    51481 tgatcggggt tttagttgga tttgtgttat cgaaggtgta tcagcagcag agggacttga
    51541 agcgtcggtc agctgctgaa gagcaagttc aacagctcac ccaaaacgct caacgagagg
    51601 ctgaaaatct agttaaggaa gcaaaactcg aagcaaaaga tctattgttt caagccaagg
    51661 ctgaactcga agcgaaagaa aaggaaaaga ggaatgagat tcaggtcgct gatcgaaaaa
    51721 tcatccagcg ggaagaggcc cttgagcgta aggtcagcca atttgaaaaa cgtgatgagg
    51781 aaatgcgacg aaaagagcgt tctcttaagg aaaaggagga agttctagac aataaagccg
    51841 tggcgtgcga ccaagccatt aaagagcacc ggcttgccct agagcaggtg gcaggaatta
    51901 ccgccgaaga agccaagcgt catttattgg gtgaaattga aagtgaagca cgtatggatt
    51961 cggcgttgtt aacaaaaaga attttggatg aggctaaaga aaccgctgaa cgagaagcta
    52021 gagagattgt gacccggtcg atacagcgaa tcacaagaga ttacgtcaat gaggccacga
    52081 tttcagtggt tcccctcgcg aatgatagca tgaagggaag gatcatcggt cgtgagggaa
    52141 gaaatattcg ggctttggag gcggcaactg gcgtcgatct tattattgac gaaaccccgg
    52201 aggcggtgat cgtgtccgga ttcgatcccc ttcggagaga agtagcaaaa attgcgttgg
    52261 aacgtctcat gcaagatggg cgtattcacc cgactcggat tgaggaagtc gtagaaaaag
    52321 tcaaaggtga tttagacaag ttaatgagag atgatgctga aaaagtcatc tttgaggtcg
    52381 gcctttcaga tttccaccct gaaattgtca aattattagg tcgtcttaaa tatcgaacca
    52441 gttacggtca aaataactta tatcatgcca gggaagcctc atatatctgt gggattatgg
    52501 cttcagagct gggtttagat gtgaagttag ctaagcgtgg cgccctcttg catgatattg
    52561 gaaaagtagt tagccatgaa gaggaaggta cacacgcgat gctcggtgcg gaattggcga
    52621 agaagtatgg agagtcagag ctcattgtga atgccattgc tgcccatcat gagcaagtgg
    52681 agccactctg cccagaatcg gtcttggttg ctgctgcgga agccctttcg gccgcccgtc
    52741 ctggtgccag acgcgaaacg ctcgaggcct atgtcaagcg attggaaaag ctggaatcct
    52801 tagccaccgg gtttacagga gttgataagg cctatgcgat tcaggctgga cgagaaattc
    52861 gggtgattat ccgtcagggg gaattaaatg acagtgaaag ttttgcccta tctcgagatt
    52921 tggccaaaaa gattgaacaa gaactgacgt atcctggcca gatcaaggtg acagttattc
    52981 gagaaaatcg ctttattgaa tttgccaaat agctctgtga gtaaaggaga acatgaacat
    53041 actgtttatc ggagatatca tgggagagcc aggccgtcgg gtgatcctaa agcatctatc
    53101 aagggttatt caggagcatc acattgatct ggttattgga aatggtgaga atgcggcagg
    53161 tggatttggt atcacacagg aggttgccga agatctgttt gatttaggtc tttcagtcat
    53221 tacattaggc aatcatgcct gggataaacg tgaagcgtta gagtatttgc aaaaagagca
    53281 gcgggtgatc cgtcccgcaa attatcccga tggggttcca ggaaaaggta cgtgtattgt
    53341 agagaccgtt catggcgagc gcctagggat tttacagctc atggggcgag tgtttatgcc
    53401 catggtggac tgcccttttc gggttgcgga acgcgaactg gctcaattaa ccacacagac
    53461 taactgtatt cttgtggata tgcatgctga aaccacatca gaaaaaatgg cgatggggta
    53521 ttttttagat ggcaaggtat ctgcggtcct tggaacgcat acacatgttc aaactgcgga
    53581 tgaacaaatc ttacctcagg gaacagggta tctaaccgat gtcgggatga ccggacctgt
    53641 tcaatccgtc atcggaatga agccaaactt ggtcattcag aaatttctga ctcaactccc
    53701 gagaagattc gaagtggcga cagggccttc tgtcttgagt gcggcggtgt taaatcttaa
    53761 tcactcaact ggaaaaacca ctcacatcac gcgtcttcgt gtgtttgagt aacctaatct
    53821 tcgtgtcagg gcttttccct tcagtactta ctgtctcgac tctcacccga cagattcgtt
    53881 caacgttcga gaaaaatttc ccaccactct gggtggaagg ggaaatttct aatcttcgat
    53941 gcccttcttc gggacaccgg tattttaccc tgaaagacca atcaagtcag atcagagctg
    54001 tgctctttcg cagtcaggtc gaacgcctga aatttgcgct tcaagatggt ttggaggtct
    54061 ttgtgtttgg ccgcctttcg gtgtatgagc cgcgaggaga ctatcaatta ctcttggaag
    54121 cggttgagcc taagggcatt ggtgcgctgc aattagcttt tgttcaactg aaagcaaagt
    54181 tggaagccga agggtttttt gagactatag ggaaaaaggt ccttcctctt tatccgcaac
    54241 gaattgggct ggtgacctca ccttccggtg cggcaattca tgatttgttg accatcattc
    54301 accgacgttg gcctctggct caaattttga tagctcctgt ttctgtgcaa ggggatgaag
    54361 ctgccggaca aatcgctaag gctattggaa cgctcaacca aattggcgaa ttggatgtgt
    54421 tgattgtcgg gcgtggtggg gggtcgttag aggatctctg gccttttaac gaagaaattg
    54481 tggtgagagc tattgcctct tccaggattc cggtggtttc cgctgttggc catgaatccg
    54541 atgtgacgct tgcggattta gccgctgatt atcgggctcc cactccttct gccgcggcag
    54601 aattggtggt tccggattgt tcaacggtta gacgttatct ggggcatcaa cgggtagggg
    54661 tggaacggtc tatgaggagt ttctgtgtgg cgtggagagg gcaaattcag gaactcaccg
    54721 gacgattgcc tgagcctaga ctcgtgcttg gacaattcgt gcaacaggtg gatgagttag
    54781 agcggcaact ttatgtaaaa atggagtctt ggtgccggag tctccatgtg cggttgctga
    54841 agcatcagtc cacgatttgg gagagaaatc ccctgattga cattcgtcgg cagcaactgg
    54901 cattgattga ccgtggcatt cgccttgctc gaggaatgtc agggtgtatt cttcttaaac
    54961 gccaccaatc agaattgtgg atgtctcagc taaatcaatt aagtcccctt gcggtcttag
    55021 cgcggggata tagcattgtt cataatcagc aaagtggaaa agtcgtgaaa caatcaactg
    55081 aagtttctgt gggagagtca gtacgcacca gacttcatga aggtgaatta atttgtctcg
    55141 tagagcgtat ccatccttca acttaaggaa ttcccttttg cttcaggttc cagcctggct
    55201 ataatacctg tttagataaa tagatgatgg tcttcatagt atagaaccga atcactgacc
    55261 ctctacggac aattttcatg gctgtactga aatttgaaaa agcattgtcc cgcttggaaa
    55321 caattgtggc cgagctggaa cgcggggagc tttccttgga tgagtccttg aggatttttg
    55381 aggagggagt caagctctca aaaacctgtc tgaaaatgct ggatgatgca gagcgcaaag
    55441 tggagatcct tgtccaagac aaggatggaa gaaagcgtat acaggctttt tcagttgagg
    55501 attccaattt gccggaatcc taggtttctt tcactaccat ctctctcaat cagtaggctt
    55561 cacattgtct gcttcgtgga catgaacata tgaaggttgt tcagccatca gccccttctc
    55621 ggaaatcctc aaaagaacga ctggactccc ttgtcctgaa tcatgagttg gtctcaagca
    55681 gggaacaggc ctgtcgcctt attcttgccg gtcgggtgaa agtcgatggg gtattgattg
    55741 ataagccagg gaaactggtt gcgacaactg tttcggtgga agtgacgaag ccggaatatg
    55801 cctatgccag ccgggcaggt gaaaagctgg caccagcctt agacgcattt tgtatttctt
    55861 gtttagattt tgtggttatg gatgtgggtg cctccaccgg aggattcacc gattgtgtgc
    55921 ttcaacgtgg cgccaatcga gtctatgcca ttgacgtggg gtatggacaa ttggattgga
    55981 ggctgagaac ggatcatcgg gttgtggtca tggaccgttg caatatccgc catctttgcc
    56041 ccaaagatat tcctgagccg gttgatttgg cggtcatcga tgtttcgttt atttcccttc
    56101 gcttggtctt gccagtgatt ttgcctgttc tgcgtgacca ggcgtatctt gtcgcattgg
    56161 ttaagccgca gtttgaagtc gggaaaggac aagtcggaaa aggaggcatt gtccgtgatg
    56221 aacgattgcg tgagcaggtg agggacggtt ttgtggatta tgcccgtagc ctgtgtcttg
    56281 atgtcattgg tgtcatggat tccacgctgt tgggaaaaaa aggaaacaaa gaaatgctgg
    56341 tgggaatgaa aaaaaacggg aagatgagtg ctgaaggaag caagccaatt gcttgcggta
    56401 gtcccctacc ctaattatgt aaaaggagaa ttctgggcat gtctacaaaa attctggtga
    56461 ccggtggagc tggattcatt ggatcacatc tggttgaccg gttaattcag gaaggcaatg
    56521 aggtcattgt tgttgataat ctctccacag ggaaaagaaa gaatgtcaat aaaaaagccc
    56581 aattttacaa aatggatatc cagagtaaac ggattgaacg ggtattccga aatgagcgtc
    56641 cccttattgt ggtccatctg gctgcgcaaa tgagtgttcg ccattccacg gaggatccaa
    56701 gctttgatgc gcaagtgaat attttaggga ccatcaatgt tttagagcat gccgtgaagc
    56761 agggtgtgcg aaaggtgacg tttgcgtcct cgggaggagt ggtctatggg gaacaggaag
    56821 tgttcccggc cgcagagtcg caccggacgg aacctctttc tccttatgga attagtaaac
    56881 tggctggtga aaagtatttg gcctattaca ccaatgctac cggactccgg tatgtggcgc
    56941 ttcgttttgc gaatgtctat ggacccaggc aagactctga aggggaggca ggagtcgtag
    57001 cgattttctc aaaacagatg ctggatggtg gtcaaccgat tgtcaatgga actgggaaac
    57061 aaacaagaga tttcgtctat gtggatgatg tggtggaagc gatcctggtg accttaggag
    57121 aagatgtgca gggaatattt aatgtgggaa ccgggcagga gacgacgatc aacgaatgct
    57181 atggaattat taaaaactta acgaagtgcc aatgcaaaga tttgtacgga gcggcaaaga
    57241 aaggcgagca attccgaagt gtgttggatg tgaccaaact ccgagaggta tttggttggg
    57301 atcctcaggt tacattggag gaagggctta cccggacagt cgattatttc cgtggaacag
    57361 aaaagtaatt aattaggtgc aggtagtagg aggatgattg ctcctggtca atcatctgtt
    57421 aatacctggg aacggattga tcgatctcaa tggaccaggc ttcaattcct ccaatcaggt
    57481 ttttgacatt cgagaatccc tgttgcaaaa gaaaccctac ggcatcggcg ctccgcattc
    57541 ctttatgaca gtatgcgaca atttcagcac tcgggtccaa ttgtttcagg gaatgaggaa
    57601 cctggcccaa gggaatcaga acggatccct ctatttttgc aatggagtac tcatgaggtt
    57661 cgcgtacatc caataaaaaa atctgatcgc ctttatccat gcgcgctttt aagtcgcgga
    57721 cggtgatggt gtaactcata ttgaaagcct ccttacgttt cttaagtaac tccttgtagt
    57781 cgaatcatca tagcggctga attttcgggg tgtcaattcc ttcttcctgc gagtgagagg
    57841 agcagaataa tgtcagaatt gccggtcgtg tcgattgaac acattgcaaa gtttgaagga
    57901 caggatatta ccatcaaggg atggatccgc aaccgccgtt caagtggaaa gttaagtttt
    57961 ttgacgctgc gggatggcac tggagatctt caggccatcc taagcaaggc agaagtagga
    58021 gaagatcaat ttatcttgag cggacaatta acccaggagt cttccgtcgt acttaccggg
    58081 aagccacgga aggatgtccg tgccccgggt ggattcgagc tggatgtgtc gaaaattcag
    58141 gttattcaaa ttgccgaacc ctttccaatc caaccgaagg aacatggcac cggatttctg
    58201 atggagtatc ggcatctgtg gctgcgttca cgacgtcaac atgccatttt acgagttcgc
    58261 catgaaatca ttcggacctg tcgaaatttt tttgatgatc gaggttttac cttaattgat
    58321 acgccgattt tcaccccaaa cgcctgtgag ggcaccacga ccttattcca aactcaatat
    58381 tttgaccagg tagcttacct ggcgcaaagc gggcaattat atggagaagc ggcggcggcg
    58441 gcttttggaa aaatatactg tttcggccca acctttcgag ctgaaaaatc aaaaacccga
    58501 cgacacctga tggagttttg gatggtggag ccggaaatgg catatgccga cctaccggat
    58561 gccatgcaac tggcggagga actggtttcc acagtcattc agcgggtggt cgatactcgc
    58621 cgacctgaat tggaaatcct agagcgggat attcctaaat tagaagccat ggtcaggccc
    58681 tttccacgaa taacctatga ggaggccctg acgcgtcttc agcagaaggg ggtcgccatt
    58741 caatttggtg acgatttcgg agcggatgac gaaacatctt tggctcaaga atttgatcgt
    58801 ccggtgattg ttcaccgtta tccggctgcc gctaaagcct tttatatggc taaagatcca
    58861 gagaggccgg atttggcgtt atgtatggat atgctcgccc ctgagggata tggagaaatt
    58921 attggagggg gacaacgaat tcatgatttg gatgaattgc tgcagcagat tcaagctcac
    58981 gaccttcctc aagaagcctt taagtggtat atagatctgc ggcgatatgg gacggttcct
    59041 catgctggtt ttggaatggg aattgaacgg gtcgttgcct ggatttgtgg gttagaccat
    59101 gttcgagaaa cgattccttt ccccaggatg ctctatacgt tgtatcctta gaatgtccat
    59161 tgggaatgac cttgaggttc ccagatattt ttggcacggc tcctgaattt ctttttccct
    59221 agctcctgcc ttttcacaac attttttgaa tgcttgacat cattggcaag ggttgtatac
    59281 ttccaggtgt gggaggaagt ggggtgattt ccacatgtat tgtaaaaatg aaacaaatca
    59341 tgaatgaatg atgttcatat accggtactc gtcgaggaaa tcagtttttg gcttaatccg
    59401 cttcctggtg gggtctatgt agattgtaca gtgggattgg gtggaacgtc gctgaaatta
    59461 ctggaaaagt ctgggaaaaa tgctcatatt attggtttgg atcgtgatta tcaagccgtt
    59521 gctattgcga aaaagacatt aagtgaatat gaatcatcag taaatataga acatgggaat
    59581 ttctcccaca tcaaagatgt tgttcagaag tccggctatg aaaaagttga cggggttgtg
    59641 ttcgatttag gggtgtcttc tatgcagttg gaccaaccag agcggggctt tagtttttcc
    59701 caaagtgggc cattggatat gcgaatggat caaactcaga agatgaccgc agctgatttg
    59761 gtaaatcacc tcccggaaaa agagttagcg gatgtcattt ttacgtatgg tgaagaacgg
    59821 tattcacgtc gaatcgcccg tgcaattgtg caggccagaa acgtcagtct tattcaaacc
    59881 acccaggccc tggtagctgt ccttgaaggc gcgctgccct atgcttacaa aaaggggcgc
    59941 ttacattttg ccactagaac ctttcaggct cttcgaatac gcgtgaatcg ggaattggat
    60001 ttgattgagc cggccctacg agatgctgtc gatctgttga aggaaggcgg aagggtgtgt
    60061 gtcgtggcgt ttcactcgtt ggaagatcgt attgtgaaac aaacgtttcg ttctatggca
    60121 aagagagagc atccaagcgt tgcattgcta gtcaagaaac ctgtgattcc aagtgtactg
    60181 gaaattcaac aaaatcctcg tgccagaagc gctaagttac gtgtggccga gcgccttccg
    60241 gaaggagtaa ccctatgagg aaaactgtgt tccttggcgt catgattctt ttgggggtgt
    60301 ggtgtctcct gtatgttggg gtcaagatag acatgtggcg cctcgggtat gccattgaag
    60361 agctggaatc tcagcgtgca gaattcaaaa aacagcagga aggcttgcag gttcggctgt
    60421 cacagttaac ggatccccaa cgagtcgctc aacgggcgca aagtcaattg ggactcaccg
    60481 tagctcagga ggggcagatt gtgatggtct ccctggatac attacccccg tctgaatctg
    60541 gtgaaaattt tcctgttcgt ttagtccgtg aattttccga taccatcgtg agccttccgt
    60601 gaaaaatccc tcaccggaca ttcatcccgt ctgtcgaatc cgaagtggca ttgtgtgttt
    60661 tgggctcctt tgcgggtttg cgcttattgg ttgtcgactt ttttacctac aggtccttca
    60721 agcggatgtt ggggctcatc aggcacaaca gcaacacgaa aagaccgtgg tggttcaacc
    60781 agatcgaggg gtaattgttg acagccaagg acatccattg gctctcaatg tggaagtggc
    60841 atccttatat gtgaggccac cgtccttgaa tgatccacaa cgagttgccc ggtcattagc
    60901 gccgatcttg cagatgccgg ccaaggaatt acgtgacctg ttcacacgaa atcagcctta
    60961 cgtttgggtt aaacgcaata ttccggaatc cgtcgtcaag gaattagagg ccttgaatat
    61021 atcaggattg gggttgacca aagaaccgca tcgtttttat ccgaagggag aattggtctc
    61081 tcatctcgtg gggtttgccg gaattgatag ccagggcttg gaaggggtgg aacttcaata
    61141 tgaatcctat ctacggggtg aaaaaaacct ggtgaaatac caaagggacg cgttgggcag
    61201 aaagcttgct tcacccccaa gtcatgattc cgtccccttg tcgactgggt accatctcac
    61261 tttgactatt gatgaggttg ttcaatttat tgcggaggcg gagttggcgc aagccttaaa
    61321 gcaaagcggt gcgaagtctg gaagcattgt gattatggat cccttaaccg gggcgattct
    61381 ggcgtgggca cttcatccca cctttgaccc taatcaccta gggcagtttt ccacgcagga
    61441 ttggcgcaat cgagtggtga cggattccta tgagccgggg tcaacgttaa agattgtggt
    61501 ggcagcggcg gcgcttgaag agggggtgat gtccccagac acgttaatct atggtggtga
    61561 tggaaagatg tcggtggcgg ggacgattgt ccatgatccg gcaaaaacca gttggatgac
    61621 gttttccgag gcgttggcgc aatcgagtaa tgtgggtgcg atcaaagtcg caatggaact
    61681 cgggcaaggc agggtatttc ggtatttgaa agcatttgga tttggagaaa aaacggaaat
    61741 tgatctcccg ggagaatcct cagggattct ccgagagccc aacaaatgga gtggtcgaag
    61801 cctctcttca attgccatgg ggcaagaaat cgccgtcacc ccgatacaaa tggtcacggc
    61861 catgtcggta gtggctaatg gtggttggct gatgacgccg tacgttgtct cctcgatcct
    61921 ggactcccac ggacaggccg tgctgacaaa agatccgcaa cccaaacggc gacccatctc
    61981 gcttgaaaca accactacgt taactcgaat tctcgaagcc gccgtagagg taggaaccgg
    62041 caaacgggcc aggcttgccg gttatcgagc tgcgggcaag accgggaccg ctcaaaaaat
    62101 tgacccgaaa accgggtcct actcttcttc gcaagtgatt gcctcatttg tcggatttgc
    62161 accagtcgag cggccacgcc ttgccatgtt agtcgtcatt gatgaaccga cgatcgggaa
    62221 ctggggggga gaaattgccg ctccggtctt tcgaaaggtc gcagagcgag tcttgcccca
    62281 tttaggagtg ttgcccggtg gatccgcagt tattcggaca gcggcggcca attcgcccat
    62341 ttcttcaccg cagcctattg tgcagtaacc tgtgacgcta cgagagcttc ttgcctcatt
    62401 ggatatggtg ctaagtgaag gagatcttca gcctgaggtt ctgtccatta ccgaagactc
    62461 ccggcaagta aagcctggtt cggtatttgt ggcaatcaaa ggggagcact atgatgggca
    62521 tcagtttgtg agacaggctc aacaacaagg ggctgttggt gtggtggtgg aagaggactt
    62581 tgagaggcat gccccactgg taggaggcgc gacagctctc attaaagtga aaaattctcg
    62641 aaaggcgttg ggtttgttgg cggcaaaatt gtccggtctg ccttctgccc atctgcacat
    62701 ggtgggagtc acggggacga atggcaaaac gacggtgacg tatttagcca aatccctgct
    62761 ggaagctcaa ggacatcgtg tggggctgtt gggaacggta ggctatgtgt tcgggaccga
    62821 acatcgtgcg gcgtcacata ccacccctgc ttctgtcgag ctacaggaca tgttaaaggc
    62881 catggtgaat gccgggtcgg atgccgcggt catggaagtt tcttcccatg cgttggcttt
    62941 agatcggata gccggttgtg aattcgacat agtcgtgttt accaatctga cgcaagacca
    63001 tttagatttt catcacacat tggatgacta ttttcaggcc aaactccgct tatttacaga
    63061 atgcgtcgat agcggacaga agacccgccc caaacgagcc cttgtcaatg cggatgatga
    63121 acgggggcca ctaattctcc aacattgttc gattccaact tggacctatg ggctccatgc
    63181 ccacgcagac atcagagcgg aagccatccg tctctccatg ggaggaacgg actttcaggt
    63241 gaacagtccc catggcctgt tgacgatcaa gagccaattg gtgggcgagc ataatgtgtc
    63301 taatatgttg gcggccatcg ggattgcact ggaaatgggc atgacggttc cgatgattga
    63361 acaagccgtg cagtcagtgg cgaatgttcc tggacgattt gaacggatca atgaaggcca
    63421 accatttacg gtcgtcgtgg attacgccca taccgaagat gccctgtatc ggttgcttcg
    63481 agcggcaaag gctattacag aaggacgaat cattaccgtg tttggctgtg ggggcgatcg
    63541 ggatgccggg aaaagaccca agatggggca ggttgcggca cgacacagcg atgtggtgat
    63601 cgttacgtcc gataatccac gaacggaaaa tcctcacaca atcctgactc aaatcgaaca
    63661 ggggattaaa gctctgcgtc cggaagaacg gtgttcgtat cagatcatcg ccgatcgagc
    63721 cgaagccatt catgccgcag tgtcagaagc caagggagga gatctgttgt tgatagcagg
    63781 gaaggggcat gaagactacc aaattcttgg cactcagaaa attcattttg atgatcgtga
    63841 agaagccagg aaggcgatcc aacgacagat gagttccagg tagctcagac cacccgatat
    63901 ggcagctttt gagatagacg aagtgctcga tgcgactgag ggaaagctga tcctccaggg
    63961 agcccagaaa cgaaaaatgc ttcgagtgca aactgattcg cgggagatta aatccggcga
    64021 tctgtttctg gccttgaaag gggagaaatt cgatggccat gattttgtga aaacggcctg
    64081 caaacttgga gcccgaggag tgattgttga agaggcgcgg gccggagaga tgttgacgca
    64141 ggtgcgtcgg gctggcttgg gtcccgtcat ggtggtggag gtaaaaaata ctctgcgagc
    64201 ctatcagggc ttagccagat atcatcggtt gcgcttttca atccctgtgg tggccattac
    64261 cggcagcaat ggaaaaacca cgacgaagga aatggtgtat caagtcctgg cgacccgctg
    64321 gcgcgttcat aaaacgcagg agaattttaa taatgccatc ggcgtaccca ggaccctcct
    64381 gggccttcac gccggacatc aggctgcggt gattgaaatg ggggtggatc aagtgggaca
    64441 gaccacccaa ttgtgtgaca tgaccagacc gaccgcaggg gtgatcacca atgtcggtcc
    64501 cgaccatttg gaattctatg gcacgatggc caggtcggcg atatcgaagg ctgaattatt
    64561 ggattggctt cctcaaagtg atggtggggc aatcctgaat gcggacgacc gatattttga
    64621 aaagttttcc cggaaggcaa aatgccctgt gatctcattt gggttctcgg cccaggcgga
    64681 tgttcgtgcg gccgactcgg aatggaacgg ccggcgaacg gcatttcgcc tgttcctacc
    64741 tcatcgtaaa ttggccaagc gggcgagtgt ccgaatcatg ggcccgcaca atattgcgaa
    64801 tgctctggct ggcgcggcag taggctgtgc catggggttg tcgggcgatg acattgtttc
    64861 tggattacat aaatttcgcc cagctccgat gcggtcggaa attcgccggt ggggtggagt
    64921 gctgtatctc tacgactgtt ataacgccaa cccggcttca gtcaaagtcg ccttggagtc
    64981 gctggttggt ctgaattcca ctcgtaggac catagccgta ttgggggaca tgcttgagtt
    65041 gggtcctaaa gaagctcagt ttcatcagga aattggacgc gaggcggcaa aactccatgt
    65101 gacccattta attgcctgtg gggcgtttgg acacaacatg tccgaaggtg ttcaaaacgt
    65161 gcacggaccc actcaggtgt ccgtggtcag ggatgccatg gaagccggag ccttgctgaa
    65221 aacaatggtg aagcgtggcg atgccgtgct cattaaggcc tctcgtggcg caaaaatgga
    65281 acgggtgctc gacgccgtcc gtcctgggtc atagcctgcc tggatcgggc cggatgccat
    65341 ggccaaaggg gtctccgata gtgaaatgtc taactgaaat gatacatgag caacgatatc
    65401 cgggcggagc cttctttccc tctccccgaa gaaagtaaat cctaatgctg tatttatggc
    65461 tgtatcccct tcataccgaa tttgccatct tcaatgtgtt tcgctatctc agtttccgca
    65521 ttatttatgc ggcggtgacc gccttcctga ttgtgttttt ccttgctcct cccatgatca
    65581 aaaaattgca aacattgaaa ctggggcaaa aaatccggac tgatggcccg caatcccatc
    65641 ttggaaaatc cggaaccccg acgatgggag gcctgctcat cattttttcg gtgttgctct
    65701 caaccgtgtt atgggcggat atcaccaatt tctatgtctg gttggttctc ttgtcgctgg
    65761 tgggtttcgg gctgatcgga tttgtggatg attacattaa aatattacgg ggccaatcca
    65821 agggattaac ggccacacaa aaaattgtcg ggcaggtagg agtggcgttg ggcattggga
    65881 tgttttttta tctctctcct ggctattcca cggaactcag tgtgccgttc tttaaatcct
    65941 tcactccgga tttaggagtg ctctatatcc cctttgccat tctggtcatt gtggggtgtt
    66001 ccaatgcggt gaacctcact gatgggttgg atgggctggc cgtcggaccg gtcattattt
    66061 cttccatggc ctataccgtg gtggcctggg ccgttggaaa taaattggtt tcaggatatc
    66121 tccttgtccc tcatatcgaa ggagcggggg aactcgccat tctgacggcc tctattgtcg
    66181 gagcgggatt gggttttctc tggtttaaca cctatccggc atccgtcttc atgggcgatg
    66241 tgggatcctt accccttggt gccgccttgg ggacggtggc agtggtgtgc aagcatgaat
    66301 tgttattgat cctggttggc ggagtctttg tgatggaggc ggtgtcggtg atttttcagg
    66361 tggcatcgtt taagtccagg gggaagcgaa ttttccttat ggcgccgttg caccatcatt
    66421 ttgaattgaa gggatgggaa gaacccaaag tggttgtgcg tttgtggatc attgcgatca
    66481 ttttgggcct cttaagcatt agcacattga aactacgcta atcagaggat tgttgtggag
    66541 ccctgtggag ccatggaagc cgtgaaagtc cctcccgtgt ttcccattgt gaattggcca
    66601 aataagcgtg tcacggtgtt tggtctcgga cgaagcggca gggcggctgc tgatctcctt
    66661 ctggacgttg gcgcaagggt gacgattgtc gaagaacaca caattcagga ttttgagtcc
    66721 acatcggccg catatggcct tcgaggtgct caggtgtttc ggggggatga agtcgccgac
    66781 gggttgaggg atctcgaact cctggtcgtc agtccaggag tccccaagga ccatcgtctt
    66841 ctcaatgaga ttggccaacg gggcatcccc attattgggg aaatggaatt agctggatgg
    66901 tttcttcgtg ctcccatcat tgccgtgacc ggaaccaatg ggaaaagtac cacggtacgt
    66961 ctgattggat cgatccttca acaaagcggg aagcgggcat ttgtgggcgg aaatcttggt
    67021 attccgttat gcgaggcggt gcccaagcgc acgaacccgt cttctacacc agactatgag
    67081 tacattgttg cagaagtctc aagcttccaa ttagaaacta ttcaccggtt tcggccctgg
    67141 attgccgcgg tgttaaatgt gacgccagat catctggatc gacatcccac tcaggaagac
    67201 tatcaggctg caaaagagcg tatttttgaa aaccaaacga ttcaggattg ggcactcatt
    67261 aatgcggacg atcccgtggt gagaaccatg gccttgtcgg cacggtcagg catttgtgaa
    67321 ttcagtctca ccaaaaaagt ggaacagggt gtctatctcg aaggaggcga tattaaggcc
    67381 aggatgcatg gagaggattt gttgttggct tcacgcgacg cccttccgat gcggggggac
    67441 cataatgtcg cgaatgccat ggcggcaatg gggatcggac ttctctgtgg atgttcagca
    67501 gaggatatgg tccgtgccct tcagactacc ccgacctttg agcatgcctt ggaggtggtt
    67561 cgggagtggc aggggatcac attcgtcaat gattcgaaag gcaccaatgt ggacgccacc
    67621 ctcaaagcct tacagagctt tcaggaacct ttaattctga ttcttggtgg aaaagacaaa
    67681 ggcggtgatt tttcgcagtt ggttgacaga atgacacggc aggttaaggg agtggtgatt
    67741 atgggagaag ccactcaaaa aatcctcaaa gcgtttgatc agatcggacc gccggtagcc
    67801 cttgcaacca gcctgacgga tgcggtgtcc caggccgtgg ccttcgcgtc caggggggat
    67861 gtggtgttgt tttccccggc gtgcgccagt ttcgatatgt ttcggaatta tcatcatcga
    67921 ggacttgaat ttaagcgggt tgtcggagaa cttcaataaa tgggcatccc agccatcttg
    67981 cggcgtaaaa actctcatgt ggattcccgg gcatgggcgg atctgttccc gagaaatctg
    68041 ggtgtcggct ccaagcggat cgatcctctg attgtgggaa taaccctggc tttgtccctt
    68101 ggggggttgg tcatggtgtt tagtgccagt ggagtcatgg cggaaaataa atttaccaat
    68161 gccacctatt atctccaacg acagatcgta tggatggtgc tgggatttag cgttctgctg
    68221 atcgggtccc tgatcgacta tcgccagtgg aagcaatgga ttcccatggt tgtcggagga
    68281 tgtgtcgtag gtttactgct ggttctggct gtgggccctc aaataaatgg tgctcgacga
    68341 tggcttgcac ttggattttt ttccattcaa cccacggaaa tggccaagct ggctgttgtg
    68401 ctgtatctcg cggcatttct ctccaatcct caacggcgag tcactgattg gcaacgaggg
    68461 tttcttcctc cggtggcgat ggtaggcatg atatgtgggc tcattgtcgt tgagccggat
    68521 ttggggagta ccgtcgtcat cggtctggta ttcgtcggca tgatgtatct ggccggcgcg
    68581 cgaatcagtc acctggggta tttaggggtg ccgatgattg tgggtgtggg ctctctcatc
    68641 tggatgagtc ctgaacgatg ggaacggatg acgacatttt taaatccttt tgcggaccgc
    68701 caaggtgcag gctaccaact ggtccagtcc atcctggcgt tggaaaatgg gggattattt
    68761 ggtgtcggtc ttgggcaggg caaacaaaaa ttgatgtttc ttcccgaggg ccataccgat
    68821 ttcgtgttgg ccctggtggg agaagagttg gggctcgttg gaacctgcgg ccttctggct
    68881 ttgtttgcca tattggtgtg taaaggcttt cgggtcgcgg ctctggcacc agatttattt
    68941 gggcgatacc tcgccttggg gatcaccatg ctcctgggta ttcaggctct gatcaatgcg
    69001 ggtgtcgtga gtgggctgtt gccgaccaaa ggtctcacgc ttcccctggt cagttatggg
    69061 gggtcgtcgc ttctggttac gatgttagct cttggaattt tgctgagtgt ggcccgacag
    69121 ggacgagcag gtccttaagt gaagagaata gtgtaatgcg ggttgtcatt gctgcgggtg
    69181 gaacgggagg tcatatgttt ccggctatcg cgtatgcgaa agaatttcaa cagcatgatc
    69241 ccgggggaag cattgtgttt gtcgggacgg gaaagtcttt agagggagaa attctgggga
    69301 aagaagggtt cgggtttgaa ccgatcacgg tagaaggttt tgtggggcga ggggtcgttg
    69361 gcaggttgcg ttctctcatt ctgctcccga attcgttgtg gcgtgcggtc aggattctcc
    69421 gaggtcacca tgccgatctg gttattggga cgggtgggta ttttagtcct cccgtcgtcg
    69481 tggcggcctg gttgctgaac attcccaggg tcttgctgga accgaatgcc atgccgggat
    69541 tggccaatcg cgtgttgggt cctttggccg acagaatttt tctggctttt gacagcgcga
    69601 agcctttttt ctcgtcatca aaggtcaagg tcattggtac acctattcga aaggcttttg
    69661 cattggaacg tcctcctgtt cctccgcaga agatctcaca cctgttgatt tttggcggga
    69721 gccaaggggc gcgggcgatt aattctgcca tgctgaatgc tgtggaatgt tcatccaggc
    69781 tgcgggagca ggtgacgatc acccatcaga ccggcgcgga cgatttcgaa cgggtcaagg
    69841 ccggctatgc acaattgggc gttcaggtgg atgtccgccc ttttctcttt gatatgcctc
    69901 aggaacttgc caaagcggat ctcatcgtct gtcgtgcggg ggctagcaca ttggctgagt
    69961 tatcagcatg tggaaaagtc ggaatcctga ttccctttcc ccaggcgaca cacaatcatc
    70021 aggaaatgaa tgccaggtcc atggaagcgg ccggagcggc tcgagtgctg ctgcaatccg
    70081 acctcaccgg gcagcggctg gcagaagaaa ttgaacaatg gatctcggat attccccggt
    70141 tacaggaaat ggctggacga agttgggcac tcaggaaaat ccatgctaca gagcagatgg
    70201 tgggtgaatg cctctcgctg gtggggcgtc cggtcgcgtc gtaataccac agatatgatg
    70261 aggaaatatg tccacaagga tgaatacgta acagggtcaa agtagaaagc attgcgtgag
    70321 tatcttcaat ctttaatggg gagggactct tacagggaat gaagagcatg tcctgcgagg
    70381 tcaatgtctg gaatgcccag ggtcgtcatg tcatgagaaa ggcctatggt cgcgttgcga
    70441 gtccggtagg ccgaatcggt attgagacca tacgtccaga cctacagacc atgtgttcac
    70501 cgcggtggat acatcaagtg accgtctccg gttatcctct cttcaactct ggaagtctaa
    70561 aacattatgt ttcgaaaaat tcagcacatt catttagttg gtatcggggg aagtggaatg
    70621 agtggcatcg cagaggttct cctcacctta gggtataagg tgaccggctc cgatgtgggg
    70681 gtgtccgaca cgatacgccg gttagaggaa ttagggggga cggtgtttat cgggcataag
    70741 gaatcgaatg tggaaggagc gcaagtggtg gtggtgtcct cggccattgc cggttccaac
    70801 ccggaaattc gtgcggcccg ggccaaggtg attcccgtca ttccacgagc ggaaatgctg
    70861 gcagaactga tgcgattgaa gttcgggatt gccattgccg gggctcatgg aaaaaccacc
    70921 acgacgtcga tggtcgcctc gattctggcg caagccggcc tcgaccccac attcgtgatt
    70981 ggaggaaaag tgaatgccat gggtacccat gcgcgattgg gcaggagtga tctgctgatt
    71041 gcggaagctg atgagagtga cggatcgttt ttgcggttat caccctccat cgtggtcgtc
    71101 acgaatatcg accgggaaca cctcgatcat tacgggaaca tggaaggtct tcaagaggcg
    71161 tttttagaat tcatcaataa gattcctttt tacggggtgg cgattgtgtg tgccgatgat
    71221 ccctggattc gcaagctcct gcctcgggtg gtgaagcgat atcacacgta tggcatgagt
    71281 gatttttcgg gtgcgctgac atctgatttg tatgcgacgg agattgaaac caaatccatg
    71341 ggtgtggaat tcagggcgca ttatcgggac caaaagcttg gcccctttcg cattcgcatt
    71401 cctggtgtgc ataacgtgtc gaacgcatta gcggccattg gggtggcctt ggaattggat
    71461 gtgccggcgg atttgatccg tgcagggttg gcggcctttg caggggtcga acgacggttc
    71521 cagattcgtg gagaaaaaaa cggcattgta gtggtggacg attatgggca ccatcctacg
    71581 gaaatccgtg ccaccttggc ggctgcgcgg gccgcctggc agcgcccgtt gatcgtcgtg
    71641 tttcagcccc atcgatttac aagaaccagg gatcttgcgg acgagttttc caaggcgttc
    71701 gaacaggcgg atcgcgtgta tgtcatggat atctatccgg ccggtgagac gccgattccc
    71761 ggaatcaacg ggcagatgat ggccgatacc attcgcgcct ctgggcaccc ttccgtccaa
    71821 tggctcaatc gtgactcggg attgatctcc aaattgcgtg aggagctccg tgatggcgat
    71881 gtgctgttaa ctctgggggc gggagatgtt tggaaagtcg gaaccgaagt gttggaatcg
    71941 ttgtaagcga gtgtggtgag tcatgaatat cgaaggacgg agaggtgaag accggccgac
    72001 caaaatggac agcgaaacgg cttcaacgag ccgtgcagca tgtgcaggga gaagtctgct
    72061 ggaacgaacc gttggcgcca cttctgtcgt tgcaagtggg aggtcctgcg gatgtgctgg
    72121 tctttccctc ggatgtggaa gacgtcattc gcgtggtcct aggggctcga acggaaggga
    72181 ttcccttggt ggtgttaggc ggaaccaatg tcgttgttcg tgataaaggg attcgaggca
    72241 ttgtgatgca attgaaacaa ttaaccgggt tgcaggagga agccggccac gtggtgtatg
    72301 ctcaggcggg tgtgcggttg ccccggttga tgcaatttgc ggtggggcat catttgtccg
    72361 gaatggagtg ggcggcgggg attcccggaa ccgtgggtgg aggtgtggtg atgaatgccg
    72421 gaacacggtt gggggaaatg caggaggttt tgaaaggcgt cgatttggtg aatcttcgtg
    72481 ggcatcgcgt acggatcgag gcgtccaaca tgtcatttgc ctatcggaag gcgaccctcc
    72541 ccaaaggtat cgtcgtaggg gcgtgggtcc agttggctct ctcggtgaaa gagcaggttg
    72601 aagcgaaaac caaaacctat ctgcggtaca ggcgggagac ccaaccgtta actcttccca
    72661 atgccgggtc ggtctttaaa aacccgcctg gggattctgc cggacggtta gtcgaagcgg
    72721 caggattaaa gggcgtgcgg gttggtgatg ccgaagtgtc aaccaaacat gccaatttta
    72781 ttgtgaatag aggaacggcc accgctgacc aggtactggc attgattcga aaagttcgac
    72841 aaaccgtggc gaggaaattt ggggtgcatc ttcagcttga atggaaaatt attggagaat
    72901 cgtagagatg ccaggcttga tggtggtgaa gaagcgattc agtcagagtg gaaggcttat
    72961 cacaaggtga aaaaacaggg aatgatgaca cagaaatctt tgcgaatcgg tgtactgatg
    73021 ggtggatcat cagctgaaag agagatttcg ctcaagaccg ggcgttcgat ctgtgatgcg
    73081 ttgacccgtc gtggatatac ggtgatccct attgaagtcg atgcctcatt gccgcatcag
    73141 atccgggcta agaaagtttc ggtcgccttt ctggccttac atggaccggg aggtgaggat
    73201 ggcacggttc aagggatgtt ggaagtgatg aagatgccgt acaccggatc gagggtgagt
    73261 gccagtgccg tctgcatgga caagggattg acgcgggtgg tccttcaagc ggccaaggtc
    73321 cccgtcccgc caggtatgac cttatccggc aaaatgatcc cggttcgtcc acctgccacc
    73381 ttaggtttgc ctattgtggt gaagccttgc gcagagggat cgacgtttgg tgtctcaatc
    73441 gtgcgcaagc cctctcaatg gaaacaggcc ttgcagaaag cctatcaatt tggagagcag
    73501 gcggtggtgg agaaatatat tcccggccgg gaagtggcgg tgggcgtctt tggcaacgag
    73561 gtgttacccg gcgtggaaat cattgtgccc ggagggtttt acgattttac ggcaaaatat
    73621 ggaaaagctc caacccgtta tgtgtgtccg gcttcccttt cttcaaaact ggaagctgct
    73681 cttcgtgatt acagtcttcg tgcctatcag gcgttagggt gtcgaggtgc tgctcgaatt
    73741 gattttcgca ttcataccaa cggacgtccg tatatcctcg agttaaacac catccccggc
    73801 atgaccgaac ggagtttgct ccccatggca gcagcacaga tcgggtggga ctatgatgat
    73861 ctggttgaac ggatattgcg tgacgccctc tttccgaaaa agatggaggc gtctgtcagg
    73921 aaagccaaga cgacaaggaa atctgcgtca tgataggaag agaatcgttg aacgcgaaac
    73981 ggcctcgaaa aaaccggccc ctcaaaaaaa taggaggacc ggtgaaagcg aaaacttcat
    74041 cgaccgcctt gaaggtccgg cgtgtcgtga tcggaggtgt cctggcctgt cttatcgtgg
    74101 tggtgctggt gggagggcga caggtgatcc gatgggccga tgagtggacg gaaatccaac
    74161 agattacggt tgtaggtttg gatcgagtca cgcgtgatga aattctgacg aggctggatt
    74221 tgccgccaca aaccagtttg ttttctgtgg atccggaact gctcgcgacc cggttggatt
    74281 cccatccctg gattgggtcg gtcgcctttg agcgggtgtt tccccattcc ctggtagtcc
    74341 aggtgactga gcgtcagccc gccgcggtat ttgggtcacc aaccgaaccc tattttctgg
    74401 atgcagaagg gtatctgttg ccgaaaggga aggcggtggc ggggacgaca ttgcccattg
    74461 ttgatggggt gacctcgaaa tttatgaccc aacatgaaga tgaaggccat cgacgcgcca
    74521 agcagggcat tcacattgcg gaattgctgt ctcagcagtt ttcgggaaga ccacggattg
    74581 atgtgtctca gcctcatacg acggtcgtgg atcttcccaa tgtccggttt caattcggtc
    74641 gtgaggtaga ggaccagtgg cagcgatttt tggtgttgta tcccaccgtg aaggataaaa
    74701 ttgaaggtcg gtcgcaagaa gtggatttgc gatttgctca aaaagtcatt tttcgcaaga
    74761 ggacactata ggacatgacc aaaaaagaac acatcgtcgt gggactggac atcggaacca
    74821 caaaaatttg tgcagtggtg ggggaaatcc aggatgatcg ctccattcat gtgatcgggg
    74881 tgggctccca tcgctcgaac gggttgagaa aaggcatggt cgtcaacatg gaaagtaccg
    74941 tggaatccat caagcgggcc gtggaggaag cagaattgat gtcggcggtc caaatcaatt
    75001 ccgtgtatac ggggattgca ggaagccata tcgggagtga aagtgcgcaa ggcgtggtgg
    75061 ctctgaaaaa acgggaagtg accaaggcgg atgtccggcg tgccgttgat accgctcgag
    75121 cctgcgccgt tccggctccg gaccgccaaa ttctccatgt gcttccccgg gaatttattg
    75181 tggatgatca ggaggggatt cgcgatccat taggcattgc aggatcgcgc ttggaagtcg
    75241 atgtgcatat tgtgacggga gcggtgacct cggcacaaaa tcttatccgg tgtgtcagcc
    75301 gggcgggatt ggatgtcgtc gatattgtgt tgcaaccgtt agcctccagt gctgctgtgc
    75361 tgacagctga agagaaggac ctgggagtcg tgatggtgga tatcggaggc ggaaccaccg
    75421 atatcgcgat cttcgtcgag ggctgtatca ggcattcggc ggtccttccc atcgggggga
    75481 gccatctcac cggggacctg gcgatgggtt tgaaaacggc gccggaggag gccgaaaaaa
    75541 ttaaattgaa atatggggtg gccagcagtc tctttgtgaa tgaggacgaa ggcatcgaag
    75601 taccgagtgt cggaggacgg ccgccgcggg tcatgcctcg agcgttagtg gctcaaatcc
    75661 tttctccacg ggtggaagaa atgttcgaac tcgtgcttcg cgaaatccgt cgtgccgggt
    75721 atgaaggcat gttggtggca ggaggcgtat tgacgggcgg gacctccctg cttccgggca
    75781 tggccgaggt ggcggaacat gtgctcaacc tttcggtgcg gttaggtcgg ccaattggag
    75841 tggaaggtct gcgggaaatt gtgagtaacc cgagctactc caccgctgtg gggctcatcg
    75901 tccattcggt cagtcacgaa aatgagtttg aactcgtggg accaggggcc ggcaaaggca
    75961 aaaaatccgg acgttgggcc ggtggggtgg tcggtaaaat gaagggatgg atcatgaact
    76021 ttttttaatt catgacaaga aggggggatc atgtatcgga gcctactttg tgagtcaatt
    76081 tgacccatta atcaaatgtc cccacgggtt atcaggggag tggtagaaag gaggagtcgt
    76141 atgttttcac tgtctgagga ggaggagtct ggcattaaca tcaaagttgt gggtgtcgga
    76201 ggagccggat gcaacgcggt caacaccatg attgaagccg gcttaagccg ggtagaattt
    76261 gtcgtggcca ataccgatct gcaggccctg ggccgctcgc ttgcatcgta taaaatccaa
    76321 ttggggccgg agcggacgcg gggtttgggg gcaggtgcca agcctgaggt tggaaaagag
    76381 tcggcattgg aaagtgaaca ccaaatccgt gaggccctgg aaggcgcgga tatggtgttt
    76441 gtcacggcag gcatgggagg tggcacgggc acgggcggtg ctcccgttgc cgcaaaaatt
    76501 gccaaagagt tgggaattct cacggtgggg gtcgtgacca aacccttcca atatgaagga
    76561 aaccggcgga ccagttttgc cgaagaagga atccgtgaat taaaaaaaca cgtggattct
    76621 ctcttgatca tccccaacca gaaactcctt ggaatggttg ataaaaatac tccgcttgtg
    76681 gaagcgttca aaattgccga cgatgtgctg cgtcaggcca ttcagggtat atcggatgtc
    76741 attaccactc cgggtttggt caatgtggat tttgccgatg tgcgaactgt catgggctat
    76801 tcgggccgag cggtgatggg gatgggaacg ggtcggggtc cgacgcgagc cggcgatgcg
    76861 gccagacaag ccattgccag tcccttactg gaagatggaa gcgtggaagg ggctcgaggg
    76921 ttgttgttga atattacggg cgggcccaat ctcacgttgc atgaagtgaa cgaagcgtcc
    76981 aacattgccc gggaagcagc ggatcctcag gccaatatca ttgttggcca agtcattaat
    77041 cccgacctgg gggatgaatt gactgtgacg gtcattgcga ccggattcga acaagccgag
    77101 tcggtcctgc ggtttccgag tgcacatcaa ccgactctcg tggtagagcc tgttaaaaag
    77161 cccgcattgg tagcggtgcc tactgaaaat ggggggagca acggttcaaa cggatctgag
    77221 gatcttgacc gccccaccta cctgcgacga cagacggctg ccaggccgat tccggaaaag
    77281 aacggcctgt tggtggatga tgattgggat gtgccgacgt ttttacggag gaaggccgac
    77341 aactaatctc atgagcgccg agaaacgtcc cctgccagtg gggaccggag tatccgatga
    77401 cggattcacg agtcatgtga tccccgtccg gcatttcttc gggaccagga gtacaccggc
    77461 agggttacat acccatatcc aactcgggca ccttgtgagt gggccgaagg aatttcccgc
    77521 tattgtctcg ctcaagcaaa tccatagtac ccgggtggtc gtcatccgac cccatagggg
    77581 actgggggca ttggagcaga ccgaagggga cgcgttagtc accaatcaac ctgagaccct
    77641 ggtagtcgtg cgcacggcgg attgtgtccc tgtgctgctg gttgagaagg ccaggggcgt
    77701 cgttgcggcc attcatgccg gatggcgtgg cgcagtggcc ggcatcgtgg cagaaaccat
    77761 tcgggcttgt gtggatgaat tcggggcaaa gccggagcac atgcatctgg cgatcggtcc
    77821 ctctatcgga ccgtgttgtt acgaagtcga tgaacaggtc attaacccgt tgcgaagccg
    77881 gtatcccgca tggcccgggg tgcttcaaga aacccacgaa ggcaaagggg tcttagacct
    77941 caagcaactg atctatcacc agatccgagc cggtggcgtt cctgactctc agattggacg
    78001 ggtagaccat tgcacccatt gtcgggacga tctcttttat tcctacaggc gggaggggca
    78061 ggtgaatggg accatgtgta gtggaattat gttgcctgcc gcataaggat tgatcccgtt
    78121 tgggggagta gtgagacttc ctgtggtgag gggaagcatc gctgacccat cctgggcgct
    78181 tcgctccatc ttggcctcca tatctggcgg agtgcagtaa ggagaaaacg tcttcttctt
    78241 agtccaggta ggggttttat tcttgtccgt gactcaagcg gcaatcccgt ttacaataga
    78301 agtcatatga aaagtcaccg gtattctcgt tgaaggatgg ccacgtttct cactctatta
    78361 cccatacgat tcagatggtc aaggagaata tccggcaggc cgcccttcgt gtcgggcgag
    78421 atccttccgg agttcggctg gtggcggcga ccaaaaccgt tcccgcggtc aatttagacg
    78481 aagcgtattg ggctggcgta cgaatatttg gagaaaacag gctccaggag gctcaggaga
    78541 aacgggaggt tctggggcca cgggaagaat tagtctggca cttcattggt cggatgcaac
    78601 gtcggaaact caaagacctt gtcgggaatt tttcgttgct gcattctgtg gaaagtctgg
    78661 aacaggccga gaggattaat gccgtggcgg aaaaactcgg gattcaacaa gccgtgttac
    78721 tggaagtgaa tgtcggggag gaagccagca agggaggatt tacctcacag gaggtggaag
    78781 caaggatgga ggacttagac cggctccctc acgtcgagat tcagggactc atgacgcttc
    78841 cgccctggca ggaaaatcct gaagacgttc gcccttattt tatacaagtc agtcggttac
    78901 gagatcgatt ggctcgacag acatggagtc gtgtacggat gaccgaattg tccatgggca
    78961 tgtctcatga ctacgagatt gcgatagaag aaggggcaac catggttcgg atcgggacgg
    79021 ctatttttgg aagtcgaggg aaagcggtta gtcccggcac agcgggatga ttcgactggt
    79081 atagcggtaa aggagaaaca tgtttattgc aggaaatatt ctacaggcga ttgcgaccat
    79141 cctggatacg attttatgga tctttatgt
//
LOCUS       DAORAQ010000002        58327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_137_length_58327_cov_63.122546, whole genome shotgun sequence.
ACCESSION   DAORAQ010000002 DAORAQ010000000
VERSION     DAORAQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 58327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58327
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_58327_cov_63.122546"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKM72_00340"
     CDS             <1..386
                     /locus_tag="PKM72_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNP59248.1"
                     /translation="LLQMIDHHWKDHLWGMDQLKDGIGLRGYGQKDPLAEYKREGYDM
                     FAGMMERIKNDTLERLFKFQLVRGERPEPEREAPRPTQLNLNRGGESASAPQQRTVQR
                     AEEKVGRNDPCPCGSGKKYKKCHGT"
     gene            623..1822
                     /locus_tag="PKM72_00345"
     CDS             623..1822
                     /locus_tag="PKM72_00345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNP59249.1"
                     /translation="MPSDHQNTQGNPDLIEKILLEIDLNGPISFARFMELALYDVPHG
                     YYMTKKVEPDLPPSDRIGWGGDFYTAPELSPILAKTLVRQVLAIDDQLGHPAQFTFME
                     IGAGNGTFAFDFIQECRSIDSDFYHRLSYQIIERSPHLQSRQAAKLSEIGEGWGNKHL
                     SWKSSLENVETDSVTGVVFSNELVDALPVHRVRMADHRLHEICVAYKGGHFVECLNHR
                     LSPNLIDYIETHKVALSEAQTSELHLAAEIWMRHVARLLRRGVVVTIDYGHTHSDYYR
                     SDRKDGTFLCYYRHAISTDPYSRVGEQDMTAHVNFSALASVGTTNGLDLIGFTTMANW
                     LIGLGVEELVRDQDQESEDVKALTQLLRPHGMGTTFKVLAQRKGMEPFPLQGLRYRAF
                     FDDAVSS"
     gene            1894..2265
                     /gene="ndhC"
                     /locus_tag="PKM72_00350"
     CDS             1894..2265
                     /gene="ndhC"
                     /locus_tag="PKM72_00350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNP59250.1"
                     /translation="MGSEPVPFNYLPIIIFMVLGVLFGVGSFVVGWIVRPSRPYRAKL
                     LPYESGAPLFMDARVQFPMRYYIIAMLFVIFDIEVVFLFPWAVVFKKLGLLGLVEMGV
                     FLAILIVGFWYAWKKGALDWD"
     gene            2383..2862
                     /locus_tag="PKM72_00355"
     CDS             2383..2862
                     /locus_tag="PKM72_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNP59251.1"
                     /translation="MGMLERQLEPNIITTNLDYCIAWCRKSALWPMTFGLACCAIEMI
                     ASVSSKYDIDRFGAGVFRASPRQSDLMIVAGTVCRKMAPVIRRIYDQMAEPRYVISMG
                     SCATSGNHYNSYSVVQGVDQIVPVDVYIAGCPPRPEALMDGLIKLQEKIQKEKYFVK"
     gene            2922..4673
                     /gene="nuoD"
                     /locus_tag="PKM72_00360"
     CDS             2922..4673
                     /gene="nuoD"
                     /locus_tag="PKM72_00360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNP59252.1"
                     /translation="MHAFLESLVNRFPQAVLTAQQDPVRLDLWAQVQADHWLEIAQFL
                     HDDPSMVLDHITDICSADYPDDLERFEVIYHFLSLSHRTRLRIKARVTEDHPEIASIS
                     GIWKGANFLEREVYDLMGIRFSGHPDLRRILMPDDYDEGYPLRKDFPAEGKGWRSRFD
                     FLPRLDEPIGEWSEAEVPDDQRHQFMADDHPGDTKRTTEMLLNMGPQHPSTHGVLRVV
                     LELDGERVSKATPDLGYLHRGVEKLAEGLTYMQMVPHTDRLDYVCAMTNNYAYVRAVE
                     KLIDMAVPERAEYVRTIVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREVLLDMF
                     EKLCGARLTLNYYRIGGVDADLTPEIITTLRDFLKTFPAHIREYDTLLQKNRIWVART
                     KGVALLSAEDAINFGCTGPVLRGSGVSYDIRKLEPYGVYDRVDWEVPLGKDGDTYDRY
                     WIRMEEMWQSARIIEQCLNQMPEGPVMAVDAQAIPPPKPEVMRDMQSLIHHFIIFTQG
                     FKPPKGETYCATEVPKGELGFFIISDGSSRPYRLKIRSPSFIHMGAFDHMARGYLISD
                     IVTIFGTYDIVMGECDR"
     gene            4760..5284
                     /locus_tag="PKM72_00365"
     CDS             4760..5284
                     /locus_tag="PKM72_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNP59253.1"
                     /translation="MLFERFKNEVDDILSRYPVKRSALLPLLNFAQREEGYVSESAMK
                     EIAKILDLTPPQVFETVTFYTMFNLKPIGKFHLQVCKSLMCALVGSDDLVMWIQKKLS
                     IAPGQTTADNIFTLSVVECLGSCGTGPMMQVNDDYYEQLSEEKVGRVLDDLKRDGTSS
                     LKTGPFMYPQPLAK"
     gene            5365..6672
                     /gene="nuoF"
                     /locus_tag="PKM72_00370"
     CDS             5365..6672
                     /gene="nuoF"
                     /locus_tag="PKM72_00370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNP59254.1"
                     /translation="MTTYEKILLKNMEQPGYTGSLADYEGTGGYRALRTVLKEMQPDQ
                     VIEVVKKSGLRGRGGAGFPTGVKWGFIPKNHPGPKYLCCNADESEPGTFKDRQLMERD
                     PHQMLEGMAITCFAIGAQTAYIYIRGEFRLGAKILEQALQEANAAGYLGKNVLGSGLT
                     INIFVHLGAGAYICGEETALLESLEGKRGMPRFKPPFPATHGLYQNPTVVNNVETMAN
                     VPHIINRGGEWFASIGSPPKSCGTRVFCLSGHVKRPGNYEAPMGITFRELIYELGGGM
                     RSDKPLKAFIPGGASAPFLTPDHLDVKMDFESVALAGSMLGSGGVTVMEEGTNMVWAA
                     LRLMEFFYHESCGKCTPCREGTSWLVQTLQRIWNKRGRPEDIGVLDELCGNIPGRTVC
                     AFGDAAVSPIVSTLLHWREEYEALIQEAQETMPRNKEIPVFSH"
     gene            6684..9374
                     /gene="nuoG"
                     /locus_tag="PKM72_00375"
     CDS             6684..9374
                     /gene="nuoG"
                     /locus_tag="PKM72_00375"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNP59255.1"
                     /translation="MATPPTTTTDMITLTIDGKAASVAKGTLVIEAARQVGVMVPHFC
                     YHPKLTPDANCRMCLVEIEKMPRLQTSCSTQVAEGMVVKSASSSVVQEARKSVLDFIL
                     ANHPLDCPVCDQGGRCDLQDFSHEYTATTSEFKELKRVFPKEYFSPLIETQMNRCVQC
                     LRCVRYCDQVMDVKALAPAGRGTMTEIKAFGAHELDCEFCGGCIQICPVGAITSRLSM
                     YEFRPWMLKRTDSICGYCGDGCQITFQTKNNDLIEVNSTHGAGRNNGDLCARGYFGYH
                     VSVHPDRLTAPLIRRGEALQSVQWEEALELVGQRLLDIKAKYGPDAIGGLITGRCTNE
                     DIYVFQKFMRGVVGTNRIDSSVRYGHLNAVHAMQRVQGTHRWAVSFDDILSADTLLLV
                     GTNITETNPITGLKVKEAVKRRGAKLITIEALQPAIGSISNIVNLAHHHLGVKPTGYA
                     GAILGMLKAVVDQQMVDPELQTNASSYVNDMTDTLKALSWNVIQAQTGLDSEMFTQAG
                     QVFAKGEKVVILVGPGVLRSEGGFGMTMNLLDLLLISGKLTKPGCGLAPLAEENNDQG
                     AFEMGGIGSVLPGGQSLGSPEERKRVAQAWGRPIPDTPGSSLPQMIEDAQQGRLKALF
                     VVGENPLASLPPSSGIQSALEKLEFLVCQELFLTETAQKAHVVLPACSYAEKDGTFTN
                     TEGHIQSVRKAIDLVGESRPDWEVFSALSVMMNDPMEYGDVKEIGKEIHNLLPGTRTL
                     GPGPLPPRPDLTVIAKYLTSDYQRDMATRYQLPANDLADDADDMILQVSQSLFHSGKL
                     SRKAKGLLQVEATGKLYLHPKDAARRGIGEGDTVKVSNRLGEVVTQVGLRERIPNGVA
                     VYPEHFDEEIRRLMPYSVDPETQVPSCKVARVRLEKMAGI"
     gene            9426..10487
                     /gene="nuoH"
                     /locus_tag="PKM72_00380"
     CDS             9426..10487
                     /gene="nuoH"
                     /locus_tag="PKM72_00380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNP59256.1"
                     /translation="MDTGIRLALTLSQIGAVMVAVLLTVAALTLLERKVLGWMQDRMG
                     PMEVGPYGILQPVADGLKLFFKEDIIPAGANKFMFSMAPVLSLVPALIGFAVIPFGPD
                     WTVDVGGVNFKPFVITDINIGVLYILAFASIGAYGIILGGWSSNSKYSLLGGLRSAAQ
                     LISYELNVGLSIVGVLLLSSSLSLVAITNAQAGGFWNWYFFGGGGFPQAVAFVVFIIS
                     SVAETNRTPFDLPEAESELVAGFFTEYSGMRFAFFFLAEYGNMILVSCVATVLFFGGW
                     HAPFEFLQGPESIHWATGIFWFVFKVSSFLFLFFWLRATLPRLRYDQLMRFGWKVLLP
                     IALANIVLTAIVAFLFPGS"
     gene            10502..11038
                     /gene="nuoI"
                     /locus_tag="PKM72_00385"
     CDS             10502..11038
                     /gene="nuoI"
                     /locus_tag="PKM72_00385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNP59257.1"
                     /translation="MKLKEWVKTLLFYEIALGMKATLSHLLHYKPITLQYPHEKKQLP
                     SNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEPTKRYSKEYYMDMT
                     RCLFCGLCVKACPVDALGMTQEYEWAVYDKRQLQLNKQQLLAIGDRNYPIREKRLEFQ
                     HPNVAYFNVGFKDIPQKE"
     gene            11116..11634
                     /locus_tag="PKM72_00390"
     CDS             11116..11634
                     /locus_tag="PKM72_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNP59258.1"
                     /translation="MGTIVFFYFASVIVATAALVVALRSPVYSALALLVMFFHVAGLF
                     VTLHAEFLAVIQILVYAGAILVLYLFVVMLLNLKAEVRFHHQLTVGLFLGCMLFAEAV
                     LLVVKGETSLGANLPAPGEPTHLTQGNTESIGELLYSTYLFPFEIASLILLVAMVGAV
                     ILTKKGILEAKS"
     gene            11634..11939
                     /gene="nuoK"
                     /locus_tag="PKM72_00395"
     CDS             11634..11939
                     /gene="nuoK"
                     /locus_tag="PKM72_00395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNP59259.1"
                     /translation="MDVPVSYYLVLSGIVFSIGLVGVLIRRNIIIILLSIELMLNATN
                     INFVAFSSYLGNLSGQVFVFFALTVAAAEVAVGLAIIIALYRHSTSTNIDNFRLLKW"
     gene            11944..13845
                     /gene="nuoL"
                     /locus_tag="PKM72_00400"
     CDS             11944..13845
                     /gene="nuoL"
                     /locus_tag="PKM72_00400"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNP59260.1"
                     /translation="MLYALIPFLPLLAFVILGLFGHWIKDRSHWVAVPAVLGSFLLSI
                     MALVDVAGGQTLHIPLYTWADSGNLHIAIGLFVDQLTVAMLLLVTIVSSLVHVYTIGY
                     MHGEPGYARFFSNIALFTFSMLMLVMSDNFLQLFVFWEAVGLCSYLLIGHWYERESAR
                     AAATKAFLVNRVGDFGFMLGILLVFVTFGSLHYQEVFPQLEQKAGDLVNLLGSVGGHW
                     EISVMTLICLFLFIGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVAR
                     FAPLFNLSPVAMDVVAVVGGVTMFIGATIALTQTDIKRVVAYSTLSQLGYMMMACGLG
                     GYIAGMYHLLTHGAFKALLFLGCGAVIIALHHEQDMKNMGGLKDKLPVTYWTFLIGAL
                     ALSGFPFTAGFFSKDQLLLAAWMSGDLGKVLAALGLLTALMTAFYTFRLVFVTFWGES
                     RVDPHHAKHVHEPSKTMTIPLMILAVLSILAGYMGIPEFLAPAFPGAEGGGGHHEGSA
                     AMGMMVTATAMGLLGIAGAYWVYVKSPGLPDRLANQWQSLYQLSLNKWFVDEAYDRTV
                     VMPTLNLADRLWKRVDIAVIDGAVNGVARAVAWGGWVTRRLQSGQAQNYALAMTVGAV
                     VILGAMIFY"
     gene            13930..15504
                     /locus_tag="PKM72_00405"
     CDS             13930..15504
                     /locus_tag="PKM72_00405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNP59261.1"
                     /translation="MVIPTGGIPWLTIVLILPLLGVVAVLLAQEALSRTIALTVSILT
                     LVVSLPLWFAFDNASAGMQFVEKHLWINSPAIHYSLGVDGISMPLVLLTTFLTPLCIL
                     VSWTSVQTRMKEFLISLLVMETATIGVFAALDFVLFYVFWETMLIPMYLLIGVWGGPN
                     RVYAAIKFFLYTLAGSILLLVAILVLFFEGGRTFDILALTHASYAPGLQSLLFWAFFA
                     AFAVKVPMFPFHTWLPDAHVEAPTAGSIILASVLLKMGTYGFLRFSLPMLPDASVAYT
                     PVIMTLSVIAIIYGAYMALAQSDLKKLIAYSSVSHMGFVTLGIFALNAQGIEGAVLQM
                     INHGITTGALFMCVGIIYERTHSRQITDNGGLANAMPKYATFFVIFALSSVGLPGMNS
                     FVGEFLVLLGTFAWSKLTGTLAALGIILAAVYILYLVQRMIYGPASPKMLPKLTDLNV
                     REFAMLVPLVVLVFWIGLYPKPLLDVMHASVARVISSQSTSVLVQQGGDGPGHTLVMT
                     ADPSGHPLIMNGEVAP"
     gene            15501..16982
                     /locus_tag="PKM72_00410"
     CDS             15501..16982
                     /locus_tag="PKM72_00410"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNP59262.1"
                     /translation="MMLPLADIVLILPEMLVVGMACLILVLDPITPAHKKEFLAWMSL
                     GILVVCSVVTASGFGQRVMAFSDLVVVDSYAVFWKMLLYLVSGLTILLSMGYLKEEKI
                     QLAEYYAFVLLALTGMMVMVSGADLLTIYLGIELMSITLYIMAGFKRFELRSIESSAK
                     YFVLGAFSSGILLYGISIIFGVTGSTRLVEIAAVVNGRGIDDPLVFIALMLLIVGFGF
                     KVAAVPFHMWTPDVYEGAPTSVTAFMAVASKAASFAAFLRVLLEAFGGIKPDWNLLIL
                     GICIITVALGNLVAIVQTNVKRMLAYSSIAHAGYALIGVVVAGWSGTEAAVSAQGVSS
                     LMLYLAIYSFMTIGAFSMVAILRKGGLEGEEIEDFTGLAKRHKGGAFLMLVFMVSLAG
                     IPPTAGFIGKLYLFMAAVNAGLAWLAIIGLIFAAISAFFYLRIVMVMYMREPSSEQEL
                     HTRLVLSPQVSFVLACAVAGVVLLGIFPGPLVSVADLSSLPIK"
     gene            complement(17039..17917)
                     /locus_tag="PKM72_00415"
     CDS             complement(17039..17917)
                     /locus_tag="PKM72_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNP59263.1"
                     /translation="MSWMSLALLAALGESLKDLLSKQGLRSVSPQLVAFAACATPIPL
                     LLGMLLLTDSIPPLGPQYIPALLLGGTLNILAMFQFMRALQSSDLSLAIPFVSLTPLF
                     LLLTSPLLVGEMPTNQDIAGIACIVAGAYVLHIQSVHRGLLGPLVAIFQQPGPRRMLS
                     VAFIYSLTSNFDKIGVQNSSPLFWSLSLTSVMTLGFILGLRFLPRPNHGIPHSRTVGI
                     LILIGLFQGIGLLLHNTALNLGPVPSVIAVKRSSMLFAVIWGILFLRERYVGQRLVGA
                     VLMMIGVGVLGLGQAA"
     gene            complement(18118..18897)
                     /locus_tag="PKM72_00420"
     CDS             complement(18118..18897)
                     /locus_tag="PKM72_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNP59264.1"
                     /translation="MPQSRGVITVKYVGIILGILLSYAFASEWNSPSPQPDLIQAIQD
                     RDVTLTHRLIDQGSDVNQRTPQGATPLHFAARSGQFTIAQLLLQNGADPGAIYQSEWT
                     PLHFAAKGGHVDIANLLLQNGALIDGLEGNITPLHIAVQEHHRRMASYLLAHGATVQT
                     SFKEGWTALHVAAQAGDTDMTRLLLDSGAPIDATNTIGLTPLHSAVLSGQRELTHYLL
                     SRGATCSLPSQPLQSSSSANFSRLYGALQEILQHCPNHPTS"
     gene            complement(19379..20020)
                     /locus_tag="PKM72_00425"
     CDS             complement(19379..20020)
                     /locus_tag="PKM72_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59265.1"
                     /translation="MDFTLIFVVLFVSLTLGGIVLTLSLSPHAKKKKEENRILGTNFI
                     PSQMFMGSDGLGGIAVNDQSLTICLLHSHAVPPRLLPISNLVGSILVKNGEVLQETLR
                     TGPKALISFQKLMHVNAERLIKSWQTPSPPKDNQRIDLIVWIHDEDEPFHVVSFLNME
                     TKEGGILYEKSLGTASHWHNVLDGLIHQADQHALVQPEPDERGTESPISVGCR"
     gene            20578..22134
                     /gene="purH"
                     /locus_tag="PKM72_00430"
     CDS             20578..22134
                     /gene="purH"
                     /locus_tag="PKM72_00430"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNP59266.1"
                     /translation="MARLGKALISVSDKTGVEKMAKGLAALEADILSTGGTAKMLRDA
                     GVAVTEVADYTGFPEILNGRVKTLHPKVHGGLLGRRSVDAHVKQMQEQGIEPIDVVVV
                     NLYPFEATITKPGCTFEEAIENIDIGGPSMLRSAAKNHEDVLVVVDPQDYERVLEALQ
                     SGTVSLGLRRELAKKVFDHTARYDSLIANYLTSKLADTAGQKFPSLLCLSYEKVEDLR
                     YGENPHQAGAVYRDRQTQEASLSQAQQLHGKAMSYNNYLDANAALELVKEFDETAVVI
                     VKHNNPCGVAIGDMPVEAYVRARETDPVSAFGGVIACNRNVDLAMAKEITSTFVEVLI
                     APAFSEEALAELQRKKDLRLLAVGALESAQRDALDMKKIVGGILVQDRDLGSLREMGD
                     LSMPSERQPTDYEYQACDFAWKVCKHVKSNAIVFATQTQTVGIGAGQMSRVDSVKLAQ
                     MKAKLPIKGCVMASDAFFPFRDGIDAAADAGITAVIQPGGSIRDKEIIQAVDEHKMAM
                     IMTGMRHFRH"
     gene            22264..23598
                     /gene="purD"
                     /locus_tag="PKM72_00435"
     CDS             22264..23598
                     /gene="purD"
                     /locus_tag="PKM72_00435"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNP59267.1"
                     /translation="MLAHSPILGMEREGKAIRVPMNVFIIGNGGREHALAWKLAQSPR
                     VTKLFCAPGNAGIAQVAECVSLQVDDLEGLKHFAQTHEINLTLVGPELPLSLGIVDEF
                     RKSHMRIFGPTRNAALIESSKSFAKELMVREKIPTPSSHTFDKLEPALTYLESCPLPV
                     VVKADGLAQGKGVIICATRAEAREAVCSMLEEQRFGKAGARVVLEEFLEGEELTVMAF
                     ADGRTVIPMLPAQDHKRIGEGDTGLNTGGMGAYAPAPLGTPELRQRILDEVLCPAVTG
                     LSRVGSPFYGVLYAGIMVKDGNPYVLEFNARFGDPETQVVLPLLKTDLLDVLEAVVEH
                     RLDQLQVEWHDQAAVCVVLTSGGYPGSYQTGLPITGLPEKESKSVMVFHAGTTHANEK
                     IVTNGGRVLGVTGLDSTLAGAREHANQALASIQFEGRYFRSDIGHRVLQVPS"
     gene            23616..24260
                     /locus_tag="PKM72_00440"
     CDS             23616..24260
                     /locus_tag="PKM72_00440"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNP59268.1"
                     /translation="MATVLELNSAPSPQQIHHLAQCLQDGGVVAVPTDSFYALAASPF
                     NEMALERLMHIKGERNHKPFPVLVGDPSQLDWLTEVIPDVARILMKEFWPGLLTLIFQ
                     ARSTLSPILTGGQGTIGIRQPNDPRVCELMTHIGPLTGTSANRSGQPPAESVGDVMEQ
                     LGAVVDVIVDGGPTPGGQPSTVLQILPKPRIIRQGAISRDRLQQILSASSIQLS"
     gene            24961..25809
                     /locus_tag="PKM72_00445"
     CDS             24961..25809
                     /locus_tag="PKM72_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59269.1"
                     /translation="MPRKRNQKRRKRKAKVPKGQRKPREKWIHLKATANAEGRVEFRF
                     SDEGTLICPEAELGSVVSYRTYSRESGKPKVIARSPGLSFDLRKNIELSYDAIAGIDT
                     NDFNFDGRHLSICSSFCSKPILKNRPDSIWVDIAPAFILEGVRENFNPEVIGWHLFLT
                     HVLPLLGLSESQRLALVVDSELSNHIPMNQRRMPYFRNHDLPTCVDILYASSDTGDYL
                     PNQLIKQCDKASRLLFSQICDGEIALPPILVGGTTEFAGYAYVNFLMTPYQIAKSSVG
                     FLGRGV"
     gene            25971..26663
                     /locus_tag="PKM72_00450"
     CDS             25971..26663
                     /locus_tag="PKM72_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59270.1"
                     /translation="MSSYKPSQSGLAPCKRDVIDLKSAADALGKTLVMGMELSFELQA
                     IAQNPKHPFHANAEEMPLQTLVLLQGMLIAERWLIEFLLGPSLRGDKKRATDICVDCF
                     NHDWRFPKGPVADELVARLSPINKLVLHPTWNLVNAAPRKWTLNRIARCIEGLDIFAD
                     GLQEKRPEVAKIFKSHVRAAHSVISPGPLSDRFCWFHPGTRMVPTAVTSPNDIGARHE
                     VRGVQRDGEPTS"
     gene            27014..27376
                     /locus_tag="PKM72_00455"
     CDS             27014..27376
                     /locus_tag="PKM72_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNP59271.1"
                     /translation="MKIVEFAFIVYPATDLARSRAFYEGVLGLTSATSMDLVDGFWVE
                     YEIGPHTLAIGKEPFLKPSGDGPQLVLEVNDFDKTVEHLRYHNVQFALEPFDLPHCRA
                     AIVMDPDGNKLGIHKRNS"
     gene            28149..30653
                     /locus_tag="PKM72_00460"
     CDS             28149..30653
                     /locus_tag="PKM72_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNP59272.1"
                     /translation="MHEFAPVHPHHHRHVAKGFRLSRFSVLLTSFLMLLINSLLATSA
                     VLPSTVAAQSAPGELDSKALYDSSAGPVFTLPPWTDASGWEVRENYATIQLADINGDG
                     HDELLGRGNNGMSVQEWDTTLGLWNEISGSGPFTNKQGWDHPQFALTIQTADIDGDGA
                     AELLGRSATGMETFKWDGTNWKLLKAEDPYWADKNGWDAAKYYSTIQTADIDGDGADE
                     LLARGVNGMDTYKWTDGEWKNLKSGSPPWSDAAGWDKVQYYSTIQTADIDGDGAEELL
                     VRAAGGMDTYKWTGSEWKYLKGGSPPWLDAYDWDDSVAYRTIQTGDLDGDGKAELIAR
                     SVDWLEVYTWTDGEWKTFFNFLAHTYWPTYQGWNNPGYYLTIQSADIDGEKGDELLIR
                     NASGMLAYKWNPTSKLLDRIVSDNPKLADDYWADGDNYLTIQTGDVDGDGAADLIARG
                     VYGIRTWSYNKVTAKAWDYPAKYGLKVFTGDETIAYDYINSYLTLAAGLTIRGQYTTD
                     ANTLKGYHTCLNHRVSGYNWTDWASLPIETCGVPGTPITPPSGLTLVTWNKVAEQIWN
                     ELVLAQAVSDYYADLLALYNTIFNNEQSILTATAGTLYNESMDEKSVDATFEDLLIAP
                     FALSSLGGDVAAAVGGILSALIVAGVDASGDDNFQGTWSDAQHELTAILGKYQHIIPA
                     GKAFVSGDSGFLQYVAEQKTSGAWDPSNAWVTRSMLSEGQRQIARWIYQTLSPSVWKM
                     YAAYFTMSPCERPPDSIVYYYVDDNAHQDCFRQIVGAEDSQLHNIMDPVSPSCAPTKE
                     NLATWEYGSCSIGEDKANFFLNRDGWKFRVKAVPAP"
     gene            complement(31289..32128)
                     /locus_tag="PKM72_00465"
     CDS             complement(31289..32128)
                     /locus_tag="PKM72_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59273.1"
                     /translation="MSFTTVFQHTSKFPTAQGAIGRLKLYGMCLLTGCLIFSGVALAH
                     DGKDTKESGTLSGERVISGTVEEIRGEQAKVDTGEAQPRYLPMNLREEKGLPALKKGD
                     RVDITVNDQNLIVDVHLEGEESNHRTVHGVLTQPLATGHEKAVIQSKEGEEETHPIRP
                     LARSKVASIPVGAEAIFLIDEMDQIVDVTFENAAEVEKIKNPEKKKSPLKGNFKQVSG
                     ILKEPLKDNKVSVQQDGKKEQQYEVRSIIQDKFKNLSSGQEVVLFVDEENKVTDVSFI
                     EKE"
     gene            complement(32275..33657)
                     /gene="nhaA"
                     /locus_tag="PKM72_00470"
     CDS             complement(32275..33657)
                     /gene="nhaA"
                     /locus_tag="PKM72_00470"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNP59274.1"
                     /translation="MDLVKEAKQTVITRPMERVISPFEDFTKLNASGGIVLLVCTAIG
                     LLWANSAFGDLYSNFWELPFTIGIGTHLLEKPLILWINDGLMAIFFFVIGLEIKREIL
                     VGELTEPRQAILPIAAALGGMVIPAFIFLLFNFQSPYSSGWGIPMATDIAFAIGLLGL
                     LGSRIPFSAKIFLTTLAIVDDLGAVLVIALFYTSEISWISLGTACVFLAILMSANRMG
                     LRSPFIYGLFGIGGLWLAFLFSGLHPTIAGVLAAFTIPATSAITKKEFAHKSEKFSQE
                     FRKAEVSGVPVLGNEEQSEAIKALETAKDLIQPPLQKLEHALYPWVTFAIMPIFALAN
                     AGVVIATDFSSLLSHSVSLGIGLGLLLGKPLGICLGAWVVVQFGSAVLPSDLGWPQII
                     GLGFMGGIGFTMAIFIATLAFQEPSDLQTAKLAILIASCLSACIGLLLLSKSTRTPTE
                     NGLHSAESPP"
     gene            34391..35965
                     /locus_tag="PKM72_00475"
     CDS             34391..35965
                     /locus_tag="PKM72_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNP59275.1"
                     /translation="MIRTLIISLGTLGFAILVVCLASFFVDEPLRRYIETNLNKNLEG
                     YTVQLEAVDFHPLGFSIDFENLTLRQETNPDPPLLIIPSWTASIQWTELIKMGIVSDH
                     VIRNAEVKFLFTQAEKEVKDKTGVEDKGWQEALYAMYPVTINTFRIEDSSFSYRDQPD
                     QPPLELTNLLLDVGNIQNVRSQVGEYPSSIHLEGSLNQTGFVSISGRANFLAEPFPGI
                     SVDFDLKNLQLQPFIPVSSLVNVEIHSGTLNGQGHVEYSPWANMAQIVRLEIENPDIN
                     YVEKHSPRKAQKAKQESPTQDTDDSKKSQDPFKVAIDSALVKNGEFGYTNQTTNPPYK
                     IFFNHVDLQVTGVGSPRITQKGELKLSGKFMGKGKTSIEGGFRPEVKHPDFDVKVKID
                     KTEMTTLNDVLKAYGGFDVKSGEFSLVSELHTDSGRVQGYIKPFFDKPEIYDLSQDKT
                     DNIFQKAYEGVVNAVASLLENVPRDQVATKTEISGNLENIKVGTWELIWNLFKNAFVD
                     AMKPAFENLKTTGRKN"
     gene            36137..36268
                     /locus_tag="PKM72_00480"
     CDS             36137..36268
                     /locus_tag="PKM72_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HNP59276.1"
                     /translation="MNEKNYVGAVLLIALLAMTGCNTMEGAGEDVQKAGEEIEKAAD"
     gene            36702..36911
                     /locus_tag="PKM72_00485"
     CDS             36702..36911
                     /locus_tag="PKM72_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNP59277.1"
                     /translation="MNKEQFKGNWNQFKGEVKKKWGKFTDDELTQIEGDYDKFKGRAQ
                     ELYGDQKEELNKWTEDWQAKNQTVR"
     gene            37096..37473
                     /locus_tag="PKM72_00490"
     CDS             37096..37473
                     /locus_tag="PKM72_00490"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNP59278.1"
                     /translation="MNITQHILKNKIASTVAAVLLMSAPAWGANGMNYSVSAEQGVQN
                     GYIHKVYNEDQFNGNWKQLKGALKENWGEFTEDDLLAIEGRTDRFEGKLQERYGDRKE
                     EVKAWVDDWMEKHPYEKTQDRQH"
     gene            complement(37570..37935)
                     /locus_tag="PKM72_00495"
     CDS             complement(37570..37935)
                     /locus_tag="PKM72_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59279.1"
                     /translation="MPFLLSGVIACQEEHEPVNTPPAQSNNLPGTVAIEPPIESDMPT
                     VSQQEGLTPGIPGNITGEVVSIEGKTYVIKNQDEREISVEVNSMTLVDESITVGDQAE
                     IRYSADNLPIAIRKMPKPL"
     gene            complement(38497..38964)
                     /locus_tag="PKM72_00500"
     CDS             complement(38497..38964)
                     /locus_tag="PKM72_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNP59280.1"
                     /translation="MNPNVAWMSLIFSISLVSGAYAEEIGSVDTKFNFLGPDHKIVIE
                     AFDDPKIEGVTCHLSRAKTGGLKGMVGVAEDTSDASIACRQVGPIRIIDELEEGEKVF
                     HESRSLIFKKLQVVRFFDKKRQTFIYLVYSDKVIEGSPKNSISTVPIRSWSTP"
     gene            complement(39234..39677)
                     /locus_tag="PKM72_00505"
     CDS             complement(39234..39677)
                     /locus_tag="PKM72_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNP59281.1"
                     /translation="MPVSLLFIFWGFILLVAPLSQALSANLDQDSYKASEQVGKSVKN
                     LQGEDIGLIEELVIGSDGKVGYAVLSFGGFLGMGDKLFAVPWASLAHPPGQEHLTLDI
                     QSKKLETAPGFNKNDWPDMKDEKWKLMIGEFYSTPEPSPHNTKAR"
     gene            39984..40415
                     /locus_tag="PKM72_00510"
     CDS             39984..40415
                     /locus_tag="PKM72_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59282.1"
                     /translation="MIYLVIEDQKISEIIAERLRKMGKKTRVISQSEELWMGIRESSP
                     DIIILDGNSPHINALEILKDLKAQEYKGQTIVLGGESSAPFTPEISRFGAIQTAGRPL
                     AASRVMGAIRIAQEHLNADLWRSSSSRSEVMRDSGQKATFL"
     gene            40486..40875
                     /locus_tag="PKM72_00515"
     CDS             40486..40875
                     /locus_tag="PKM72_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59283.1"
                     /translation="MPNTNASFSAFSRVDQLKQQLEKLENLSQTEVDAKELDNFDRET
                     EKILKRVDGRDTKMAAYSFATMAEAEALVNLPESAQEPTSRDLLQKSLQQRRQVLLGM
                     VSEMEGMEEAEAEVLTGEDKEDPPLMG"
     gene            complement(40970..41971)
                     /locus_tag="PKM72_00520"
     CDS             complement(40970..41971)
                     /locus_tag="PKM72_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNP59284.1"
                     /translation="MPGLEEIIAGCIVISLTLYALLGGADYGAGIWYLLARGPEAQTQ
                     RHLISTAIGPIWEANHVWLILVITLLFSAFPLAYARISTVLHIPLTLLLTGIVLRGSA
                     FAFRSYDVQQSPLHRFWGTLFGLSSLITAMFLGIILGTLTSSYLQNVQGGFVDVFVLP
                     WIQPFPLAVGIWTITLFAFLASTYLIHETPLPALRGLYRRKALWSLAASAIMAILTLL
                     LTKSYASEISQGLLQSPTGWVFLILALLGMLASIIGLWTKRFHLARISAVGYVTAIIW
                     GWGFGQYPYLIKPDLTIFNTTAPASTLELLLFALVAGMIVVLPSLLYLFRVFKKIQV"
     gene            complement(41958..43304)
                     /locus_tag="PKM72_00525"
     CDS             complement(41958..43304)
                     /locus_tag="PKM72_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNP59285.1"
                     /translation="MIENLLAARLQMAISLGFHIIFAVAGMAMPLLMVIAEWRYIRTG
                     NEIFRELATRWAKGTAILFAIGAVSGTVLSFELGLLWPTFMAWAGPIIGLLFSLEGFA
                     FFTEAIFLGIYLYGWKKIPAYLHLMSGVIVTVSGVLSAFFVVLANGWMNHPVGFQLLD
                     GQPIQIAPLATFTNPIGLHESHHMILAAFVAIGFLVAGVHAFFLLRQPENPFHQEALR
                     IALMTGGVAAIFQPLSGDLLARAVATYSPIKLAALEGHFHTTSGASLWIGGIPDGSTA
                     TISYGIEIPYALSLLLHGDPMATVTGLLDFPRQDWPPLVVVHLAFQIMIASGFILMAM
                     SVWGFCKIWKQKFLWKSRWFLRCLVGCAPLGLIAIESGWVVTEVGRQPWIIVGIMRTT
                     DAVTPMTGLTVSLIFFTLLYLGLAEIVLWLLHRHLTVFPQIGEPDGPSSRKTHARA"
     gene            complement(43406..44011)
                     /locus_tag="PKM72_00530"
     CDS             complement(43406..44011)
                     /locus_tag="PKM72_00530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNP59286.1"
                     /translation="MPLNLAIIYGSVRTDRQGIKAARFMLNTCQTRGHTASLIDPLLF
                     PLPLLDKMYKEYQPGQAPHLLQLVADLIIPADGYIIVSGEYNHTIPPALSNVLDHFLE
                     EYFRKPSAIVCYSAGGFGGVRAAMTLRAMLAELGMSSIPSLFPIPHVQDAFQEDGTPA
                     DQKFHHKAREFFEELEWYAHALKQARAHHCPRSESSAQSLT"
     gene            44308..44946
                     /locus_tag="PKM72_00535"
     CDS             44308..44946
                     /locus_tag="PKM72_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNP59287.1"
                     /translation="MISNWIDISMPLYPGMVQRPGDPPFQMEPVTDRKNGDKVPVSKI
                     SMSSHAGTHIGAPVKCEKSFQDNEQTEFSATIGLAKVIEIQDRNSVKAEELKKHRIYS
                     HDRILLKTRNSASDWYSEHPPEDTIFLDRTAAAWLAERRVSTVGIDALFIGGKHQNGK
                     EIARMLIQAGIVIIEGLEFSRVKPGEYMLICLPLKIPNGDGAPARAILQPVR"
     gene            45094..46242
                     /locus_tag="PKM72_00540"
     CDS             45094..46242
                     /locus_tag="PKM72_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNP59288.1"
                     /translation="MPEIHQKEFSEFERKASSPPPSSEIEAGYLSPLFQGPINIRSIA
                     LTGLFVYASLMVMYVAKAIMLPVFVALFLNLLFAPLVRGVEKIHIPAPFAAGGILLVL
                     ISTLTFGIIQLSTPASLWLDRAPDALQQVEHKIRSIKSSVLEMGEATKALEHMASLSE
                     SRKAQNIQVKTESLGGQLIYWTTEFILGLVSTMILLYFFLASGDLFLEKLVKVLPRFS
                     DKRRAVEIVRGIEGNISSYLVTVTSVNVGLALATSLAMYLLGMPNPFLWGAMAGILNF
                     IPYVGSIIGLGAVTLAAILTFEHLNFVAVVSGTYLFLTAIEGSVITPHLLGRKLTLNP
                     VIILVSVLFWGWLWGVVGALLAVPFVASLKIICDQIEPLYSIGEFLSR"
     gene            46330..47706
                     /locus_tag="PKM72_00545"
     CDS             46330..47706
                     /locus_tag="PKM72_00545"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HNP59289.1"
                     /translation="MPAWVLNWLNKLRSSLWLIPTCMTLAAILLSLVVPYFDKYFSPG
                     NIDFLQWLSNMGPEGAMTLLSTIAGSMITIAGVVFSITIVALTLASGQFGPRLLGNFL
                     RDRGNQVVLGTFVATFLYCLLVLRTIHVDPDGSTPYWGTLAGLILAVCSLGVLIYFMH
                     HIAVSIQAPNLIAAVYGELEEDLARLFPERLGLADENEANKTRIPSILSRIEKEGMEI
                     KADIGGYLQAIENDALMEIAREQNLVIFLHYRPGHFITKGASLAKVLALGPMADVVTP
                     QINKQMICGSNRTQEQDIEFSVLQLVEVAVRALSPGINDPFTCVHCIDHLSAILCQLG
                     QRKFPSPFRYDSEGVLRVIAPSVTFEGVMNAAFNQIRQNGKGNVAVTICLLDGLGRIG
                     ETAERNEDRVAILRHVEMVQRGSRESIQEKNDLEAVEDRCRQVMASLSMGESRSSFLM
                     DKLLHILP"
     gene            48046..48819
                     /locus_tag="PKM72_00550"
     CDS             48046..48819
                     /locus_tag="PKM72_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNP59290.1"
                     /translation="MLKTLLEMRGYQLRAIDKDLGSVDDFYFDDRLWRTRYLVADTGN
                     WLPGRKVLIGREALERSEWEDKVIPVTLTSIEIEDSPGIETDLPFSLQKEKELRLFFK
                     WREYWDEDGFIQPAGFTPMSVPGGEAMEQSLTDGMRAPGKLDGNPHLRSANEVQGYYI
                     GAQDGEIGHVDDFIVEDTSWEIRYLVIDTQNWLPGKKVLISPKWVNSIDWNKQTVQVG
                     MNRDLIEQSPEYDPSKPINREYEVRMYDFYGKPHDWVEK"
     gene            49002..49613
                     /locus_tag="PKM72_00555"
     CDS             49002..49613
                     /locus_tag="PKM72_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59291.1"
                     /translation="MLNTLTNNPVPTVLIGLGLYWLFKDGAEPSHETTRRHRSDNNPW
                     RNGEKELQPSMEEQVTEKFRTAKDAVQGQVSEWKDEASHQAHELKEQASQQAKEWKEG
                     AGQKIEEVKGYLQEKKAVVGGEFNHLLDSNPLAVGAVMVALGTAVAAAIPASRKEDQW
                     MGDSRDRVMKAVKSSVESTVEDVKQKTGQVAEKVFSEDTKNFL"
     gene            49651..49833
                     /locus_tag="PKM72_00560"
     CDS             49651..49833
                     /locus_tag="PKM72_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59292.1"
                     /translation="MTSYAKRISLVLCFSGSLFGALGCEQEGPAERAGERVDESMEQA
                     GEKMEQAGENIQDSAN"
     gene            50256..50648
                     /locus_tag="PKM72_00565"
     CDS             50256..50648
                     /locus_tag="PKM72_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59293.1"
                     /translation="MFTPNHKTQWFLDVKIIMAFAVLMGVPGFAVGSDLTGHAPDHNR
                     STTTQIQEDRQLDGGHRIIQGVVGEVNKNTVRVDAGEAGEMSPRYLNLNNSEGKENFE
                     VGDRVKIEVNAQNKVVKFHPLNRTEGKQ"
     gene            50740..50904
                     /locus_tag="PKM72_00570"
     CDS             50740..50904
                     /locus_tag="PKM72_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNP59294.1"
                     /translation="MLSWAITFLIIALIAGVVGLTGVAGTATNIAWVLFVVFLIIFAL
                     SFITGKRPPA"
     gene            51053..51568
                     /locus_tag="PKM72_00575"
     CDS             51053..51568
                     /locus_tag="PKM72_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59295.1"
                     /translation="MIMIITPDETFRKAMVLHFQEKEYELSIPERRQDVPSLVEKHAP
                     DVIVLDMSIADPNGPTVLKDLRGTGYTGKIVVISGPSNRPLMVDIHQQKVDQVMRKSP
                     TDPIPSLMDQLESIIRVMFREEISKKAYDHYNKRLGQHGKDWEDWFKAKEEILKNPTG
                     GRAPSNGPQQP"
     gene            51655..52515
                     /locus_tag="PKM72_00580"
     CDS             51655..52515
                     /locus_tag="PKM72_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNP59296.1"
                     /translation="MTRPIWKGHITFGLVTIPVVVHSAEKQFDLHFRLLDSRNNARVR
                     YERVNEETGEEVPWQDIVKGFEYEEGRYVLLNDADFEKVAIEVNKSVEIEGFVNGKDI
                     GYEYFDKPYYLVPDKKGEKVYVLLREVLRRSGKIGIAKVVIRTRQYLAALIPQGHVLV
                     LELLRFHQEIRDVKELDVPEGNVKSYKISEKELTLAQQLVESMSTKWEPQRYHDDYRD
                     ALLKWIEKKSRSSRSRTSVPPVAKEEKTGSGKVVDIMSLLKKSVQKAQPSVRAKKRKS
                     ASPRKSRKAS"
     gene            52615..55239
                     /gene="ligD"
                     /locus_tag="PKM72_00585"
     CDS             52615..55239
                     /gene="ligD"
                     /locus_tag="PKM72_00585"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HNP59297.1"
                     /translation="MGLKEYQGKRHFEKTPEPRGTAFRKKSSSFVVHKHAASRLHYDF
                     RLELDGVLKSWAVPKGPSLFPSLKRLAVHVEDHPVEYGSFEGTIPKEEYGGGTVMVWD
                     RGQWVPQGDPQYSYDRGRMKFLLKGQKLKGGWSLVRMGGAKAKEEKNWLLIKEKDSEA
                     LRGKHSDIIDMAPGSVATGRSLEEIAEVNQEKRRKNPASQREPKNNRSTKSRSSRTPS
                     RKNIISGTSRVPDPSDLPSAVSSSQPATFHPQLATLFKTSPEGEDWLHEIKFDGYRIV
                     SMVKNGRIRLLTRNGKDWTKKFPDIAGALKALPVQQGILDGEVVVLRSNGTSDFQALQ
                     NVLKGKASDPLVYFVFDLPYCEGYNLCRTPLIKRKELLWEFIKALPEKAAAHVRYSDH
                     IQGQGERTFEQACRRALEGIISKNAMSPYRQARSKHWVKVKCHHRQEFVIGGYTNPSG
                     SRKFFGALLLGYYDKEGHLQYAGRVGTGFTHQRLSRIYALLAKRAQSHPPFARLPSDR
                     NIREVHWVHPELVAEVEFFEWTRDGILRHPSFVGLREDKPAKEIIKETPHPVNNILSE
                     PLKPSNRRSKASPDISTEKPALALTHPERILYPQQGITKRNLAEFYQQIAKWVLPHVA
                     GRPLTLVRCPEGSQKKCFYQKHASGSLPDSVHRILIREKETKTKNTYLVIDDVKGLIA
                     LVQMGVLEIHPWGCRKDRTDRPDRLVLDLDPGPGVKWEQLVEGAHMLKKRLSGDGIES
                     FVKTSGGKGLHVVAPLTRRATWDQLKQYTRRLAMETARTHPSGFVATMSKAKRKGKIF
                     IDYLRNSFGATSIATYGTRALPGAPVSTPVSWDELFSISGSQVFTLTTLPQRLQNLSA
                     DPWKGFFDLRQTLSRSLI"
     gene            complement(55292..55597)
                     /locus_tag="PKM72_00590"
     CDS             complement(55292..55597)
                     /locus_tag="PKM72_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59298.1"
                     /translation="MPRGDKSKYTGKQKRQAEKIEEGYEKRGVPEDEAERRAWATVNK
                     MTGGGKKPGGSGRGKVMNTAPAKKGGEKGGKASASRSASAKSASARKAAETRARKKK"
     gene            complement(55723..56724)
                     /locus_tag="PKM72_00595"
     CDS             complement(55723..56724)
                     /locus_tag="PKM72_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNP59299.1"
                     /translation="MSQDNTAEQKSGTRGRNATQPSQIPNAGWWDILLRLKDQISQDN
                     VSIVAAGVAFYAVLALFPALAAIVSLYGLFTEASDIQQQITSLSSFLPHDTQTLIQEQ
                     LTTISTSGESALSFGAIGGLLFAIWSASKGMAAMITSLNIAYNEEEERSFIKVTALAL
                     GLTVGGLIFVILTLFLITLLPAVLDALGFGPKMQTALSLIRWPLLAMIVMGGLAILYR
                     YAPCRNYPRWQWVSWGAGIATLMWIVGSSAFAIYANDYSSYNQTYGSLGAAVILLMWF
                     WLTAFIVMVGAEINSEMERQTRADTTEGEPEPMGQRQAYSADTLGRTAETHTHSTRS"
     gene            complement(56826..57707)
                     /locus_tag="PKM72_00600"
     CDS             complement(56826..57707)
                     /locus_tag="PKM72_00600"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HNP59300.1"
                     /translation="MDDQPTQLNALESKKIEDPARLREQIRVTRAELDGTVHTLQERL
                     SPETIKEQVKTAAMDKVETVKENARVKVNQWQSILVERVLNNPLPAALIGVGLLWMMK
                     QAGTSSSARGRFGRDRYGDDYSLDAYEEWPDYAPESEAGRIPRQRSRRGRSPLDAIKA
                     RAQTSGQQAREQISEWTDQAQESLEDWKEKASHRSDEIRERTREGGERMKHELFRYMQ
                     ENPLTIGAVTLAMGTAIGLSLPRSEKEDQWMGETRDRLLEEAKATAKEIMPKAKEAAA
                     EAQRVAAETIKEQVGMN"
     gene            complement(57683..58075)
                     /locus_tag="PKM72_00605"
     CDS             complement(57683..58075)
                     /locus_tag="PKM72_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNP59301.1"
                     /translation="MNLYQEERPLSDLFRDLVNETKILIQQELQLLKLEMSQKAKQAG
                     KNVGFIAVGGALAYAGLLVLLGGATLALALIIPAWASALIVGLTVVGIGYALIHKGIS
                     DLKTMDPAPQKTIDSLKETKQWTTNLLN"
     gene            complement(58125..>58327)
                     /locus_tag="PKM72_00610"
     CDS             complement(58125..>58327)
                     /locus_tag="PKM72_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59302.1"
                     /translation="LNETEMFARRHPEAFLGGAFMLGLVAGRFLKSSRDRRIDESSSY
                     GRQESFRREEATATTTPAAPTM"
BASE COUNT        14567 a        13206 c        15397 g        15157 t
ORIGIN      
        1 ttctgcttca aatgattgac catcattgga aagatcactt gtggggaatg gaccagttga
       61 aagatggaat cggcctgcgc gggtatgggc aaaaggatcc cctcgcagaa tataagcgtg
      121 aaggctatga catgtttgcc ggaatgatgg agcggattaa aaatgatacc ttggaacggc
      181 tctttaaatt tcaactggtt cgcggtgaac ggcctgagcc tgagcgggaa gcgcctcgcc
      241 cgacacaatt aaatctgaac cggggaggtg agtccgcttc cgctccccag caacgtaccg
      301 ttcaacgtgc cgaagagaaa gttggtcgaa acgatccctg cccatgtggg agcgggaaaa
      361 aatacaaaaa atgccatggg acctgacccc tgaaaggcct gccctgctct tctcattgac
      421 gcgagtattg ttgtaacctc accctctttc tgtttgccac tcacctacct tcttcttccg
      481 attaggttct tgttcataac gtgttgaatc tattgttttt tattgccata aaaagtgcga
      541 aattgcttga cttgtttaaa ggtaggccgt tatcgtaacc gattctcaat gggcatattg
      601 caggagggac catagtccat tcgtgccatc agatcatcag aatacacagg gtaatccaga
      661 ccttatcgag aagattctgt tggaaattga ccttaatggg cccataagtt ttgcgcgttt
      721 catggaattg gcgctctacg atgttccaca cggatattat atgaccaaaa aggttgaacc
      781 tgatctccct ccctcagacc gaatcggatg gggtggtgat ttttatacgg caccggaact
      841 ttcccctatt ctggcgaaaa ccttggttcg ccaggttttg gctatcgatg accagttagg
      901 acatcccgct cagtttactt ttatggaaat tggtgcaggg aacggaactt ttgccttcga
      961 tttcattcaa gaatgccgat cgatcgattc tgatttttat catcgccttt cttatcaaat
     1021 catcgagcgg agtccccatc tccaatcgcg tcaggcagcc aagctcagcg agatagggga
     1081 gggctgggga aacaagcatc tgagttggaa gtcgtcgtta gagaacgttg aaacggattc
     1141 ggtgaccggt gttgtctttt ccaacgaatt agttgacgcg ttacctgtcc atcgagtccg
     1201 aatggctgac caccgccttc atgaaatatg tgtggcctac aaaggcgggc attttgttga
     1261 atgtctgaat cataggctct ctccgaacct gatcgactac atcgagactc ataaggtggc
     1321 cttgagtgaa gcccaaactt cagaactcca tcttgccgct gagatttgga tgaggcatgt
     1381 ggctcgcctt ctccgtcgcg gtgtcgtggt gactatcgac tatggtcata cgcatagtga
     1441 ttattatcga tccgaccgaa aggacgggac cttcttgtgt tattatcggc atgccatttc
     1501 gaccgatccg tattcaaggg ttggagagca ggatatgacg gctcatgtca atttttccgc
     1561 tttggcgagt gtcggaacga caaacgggtt ggacctcatc ggattcacaa ctatggctaa
     1621 ttggctgatc ggattggggg tcgaagaatt ggtgcgagac caggaccaag agtctgaaga
     1681 tgtaaaggcg ttgacgcaat tattgcgtcc ccatgggatg gggacaacat ttaaggttct
     1741 cgctcagcga aagggaatgg agcctttccc gcttcaaggt ttgcgttatc gggctttttt
     1801 tgatgacgcg gtgtcctcat aatgaagatg gaaaaagttt tgaatcggag atgaatgtgg
     1861 tgagtatgac tcattctgga atgagagtaa tctatgggta gtgaaccggt tccttttaat
     1921 tatctcccca tcatcatctt catggttctt ggggtgctct ttggagtcgg gtcctttgtc
     1981 gtggggtgga ttgtccgtcc aagccggcct tatcgggcga aactgcttcc ttacgaaagt
     2041 ggcgctccgt tgtttatgga cgccagggtt cagttcccga tgcgctatta catcattgcc
     2101 atgctctttg tcatttttga tattgaggtg gtgttcttat ttccttgggc ggtggtgttt
     2161 aaaaagcttg gccttcttgg cctagttgaa atgggagtgt tcctggccat tctcattgtg
     2221 gggttctggt atgcctggaa aaaaggtgca ttagattggg attaagaaaa ataggtcttt
     2281 acgcctggaa aaaaccggag ttcacccagt acaatgacac ccccattgtt tgggggtagg
     2341 cagggagcca catacagaac attgaggtag gcagataatc ttatgggcat gcttgaacga
     2401 caacttgaac cgaatattat tacgaccaac ttggattatt gtattgcctg gtgtcggaaa
     2461 tctgcccttt ggccgatgac cttcggatta gcctgttgtg cgattgaaat gatcgcctcc
     2521 gtgtcatcca agtatgacat tgatcggttt ggggccgggg tatttcgtgc gtcaccacgc
     2581 caatccgatt tgatgattgt ggctggtacg gtttgtcgga aaatggcacc ggtgatccgt
     2641 cgaatttatg atcaaatggc cgagcccaga tatgtgattt ccatgggatc ctgcgcgaca
     2701 tccgggaatc attacaatag ttacagcgtc gttcagggtg tggatcaaat cgtccctgtg
     2761 gatgtctaca ttgcgggttg tccaccaagg ccagaggcgc tcatggatgg tcttattaaa
     2821 ctccaagaaa aaattcaaaa agaaaaatac tttgtgaagt aacaccggaa gttgtgtaga
     2881 gggtttttcg ataatcaacg atccagcaac cgatctcatt tatgcatgct tttcttgaat
     2941 ccctggttaa ccgctttcct caggcggtgc tgacggcgca gcaggatccc gtgcgtttgg
     3001 acctgtgggc tcaagtgcag gccgatcatt ggctggaaat tgcccaattt ctccatgacg
     3061 atccctcaat ggtcctggat catatcaccg atatctgttc ggcggattat cctgatgacc
     3121 ttgagcgctt tgaagtcatt tatcactttc tctccctttc acacaggacc cgccttcgaa
     3181 ttaaagctcg cgtgacagag gaccatccgg agatcgcctc tatcagtggg atctggaaag
     3241 gggcgaattt cctggaacgc gaagtgtacg acttgatggg catccgcttt agcgggcatc
     3301 ccgatctccg acgaattttg atgcctgatg attatgatga agggtatccg ttacgaaaag
     3361 attttccggc cgaagggaaa gggtggcgaa gccgctttga ttttctcccc aggttggacg
     3421 aaccgattgg cgaatggtcg gaagccgagg tgccagatga tcagcggcat caattcatgg
     3481 cggatgacca tccgggtgac accaaacgca cgacggaaat gctcctcaat atgggaccgc
     3541 agcatcccag tacgcatggt gtgcttcggg tcgtgttgga attggatggt gagcgggtca
     3601 gcaaagccac ccccgatctt gggtatctgc accggggagt ggaaaagttg gctgaaggtc
     3661 tgacgtatat gcaaatggtt ccgcataccg accgattaga ctatgtctgt gccatgacca
     3721 ataactatgc gtatgtgagg gctgtggaga aactgataga tatggccgtt ccggaacggg
     3781 cggaatatgt gaggaccatc gttgccgaaa tgcaacggat catcggccat ttattctggc
     3841 tgggaacaca agccctggat atcggagcca tgaccgtgtt cttttggaca ttccgggaac
     3901 gtgaagtctt gttagatatg tttgaaaagt tgtgtggagc ccgtttgaca ttgaattact
     3961 atcggattgg aggcgtggat gcggatctca caccggaaat tattacgacc ctcagggatt
     4021 ttttgaaaac gtttcctgcc cacattcggg aatacgatac gctcttgcaa aaaaatagaa
     4081 tttgggtagc ccgcacaaag ggggtggcac tcctttctgc ggaggatgcc atcaatttcg
     4141 gatgcaccgg gccggttctg cgtgggtctg gagtgtccta tgatattcga aaactggagc
     4201 cctatggcgt gtatgatcgc gtggattggg aagtccctct gggaaaagac ggggatacct
     4261 atgaccggta ttggatcaga atggaagaaa tgtggcaaag cgctcgcatc attgaacaat
     4321 gcctcaatca aatgcctgag ggacccgtga tggccgtcga tgcccaagcc attccccctc
     4381 ccaagcccga ggtgatgagg gatatgcaaa gcctgattca tcatttcatt atctttacgc
     4441 aagggtttaa gccaccgaaa ggcgaaacgt attgtgcgac tgaggtgcct aaaggtgaat
     4501 tgggcttttt tatcattagt gacgggagtt cgcgtcccta tcggttgaaa atccgatctc
     4561 cttcctttat tcatatgggc gcctttgatc atatggcgcg tggatatttg atttctgata
     4621 tcgtcacgat tttcggaacg tacgatattg tgatggggga gtgtgatcga taacatgggg
     4681 aatggtgtca gggagttaaa gggttacgcg cacaatggtc attgggcgat gactcctcac
     4741 ctcaactcac gaggctgcca tgttatttga gcgattcaaa aacgaggttg atgatattct
     4801 gtcccgctat ccggtgaagc gctctgcgtt gttgccgtta ttgaattttg cgcagaggga
     4861 agagggatat gtcagtgaat cagccatgaa agagattgcc aaaattttgg atttgactcc
     4921 cccgcaagtg tttgagaccg tcacgtttta tacgatgttt aatctcaagc cgatcgggaa
     4981 gtttcacctt caagtgtgta agtcgctcat gtgtgcttta gttggatcgg atgatcttgt
     5041 gatgtggatt cagaaaaaac tgagcattgc cccaggtcag acaacggcag ataacatctt
     5101 tacgctcagc gtggtggagt gtcttggatc ctgtgggacc ggtcccatga tgcaggtgaa
     5161 tgatgactat tatgagcaat tatctgaaga aaaggtcgga cgagtcctgg acgatttgaa
     5221 acgggacggg acaagttcgc ttaaaacagg acccttcatg tatcctcagc cccttgctaa
     5281 atgacaggag tctttgtaga gcatagacgt ggataagcaa ttcatgggtt attcaaaacc
     5341 gcccgtttac gaattaatgt gtttatgacc acttacgaaa aaatattatt gaagaatatg
     5401 gagcagccgg gatacaccgg ttcgttggcg gactatgaag ggaccggtgg gtatagagcc
     5461 cttcggacag ttttgaagga aatgcagccc gatcaggtga ttgaggtggt gaaaaagtca
     5521 ggtcttcgcg gtcgtggtgg agccgggttt cccaccggtg tgaaatgggg attcattccc
     5581 aagaatcatc ccggtcctaa atatttgtgc tgcaacgcgg atgaaagtga acccggaaca
     5641 tttaaagacc ggcaactcat ggaacgggat ccccatcaaa tgctggaggg gatggccatt
     5701 acctgtttcg ccattggcgc acaaacggcc tacatctaca ttcgaggaga gtttcgtctc
     5761 ggtgcgaaga ttctggaaca ggctcttcag gaagccaatg cggccgggta tcttggcaaa
     5821 aatgttctgg ggagcggatt gacgatcaat atttttgtcc atcttggagc cggagcgtat
     5881 atttgtggtg aggaaacagc tctactggaa tcattagaag ggaaacgggg catgcctcgc
     5941 ttcaagccgc cgtttccggc gacgcatggg ctatatcaaa accccactgt ggtcaataat
     6001 gtcgaaacga tggccaatgt tcctcatatt atcaatcgag ggggagagtg gtttgcctcg
     6061 attggctctc cgcctaaaag ctgtggaacg cgggtgtttt gtttgagtgg acatgtaaag
     6121 cggcctggca attatgaggc tcccatgggc attacattcc gcgaattgat ttatgagttg
     6181 ggcggaggga tgcgatcgga taagcccctt aaggccttta tccctggtgg ggcctcggct
     6241 ccatttctga cgcctgacca tctggatgtg aaaatggatt ttgagtcagt ggcattagcc
     6301 ggctctatgc tgggatccgg gggcgtgacg gtgatggaag aaggcacgaa tatggtgtgg
     6361 gccgctctcc gattaatgga atttttctat catgaatcgt gcggaaaatg tacgccctgc
     6421 cgggaaggaa cttcctggct cgttcagact cttcagcgaa tctggaacaa acgggggcgt
     6481 cctgaagata ttggagttct tgatgaactc tgcggaaata tccccggccg tacggtttgt
     6541 gcgtttggtg atgcggcagt ctctcccatc gtgagcacat tactccactg gcgcgaggaa
     6601 tatgaggctc tcattcagga agctcaggag acgatgcctc gcaacaagga aattccggtt
     6661 ttctcgcatt gaggatatct gaaatggcaa cacctccaac gaccactaca gacatgatta
     6721 ccctgacgat cgatggaaag gcggcatcgg tcgcaaaggg gacactggtc attgaggctg
     6781 cgcggcaggt cggtgtcatg gtgccgcact tttgctatca cccgaaattg acgccagacg
     6841 ccaattgccg gatgtgttta gtggaaattg aaaaaatgcc tcggttgcaa acctcctgta
     6901 gtacgcaagt ggcggaaggc atggtggtga aatcggcgtc ttcctctgtg gtacaagagg
     6961 cacgcaagtc tgtattagat tttattttgg ccaatcaccc gttggattgt ccggtctgcg
     7021 accaaggggg gcggtgcgat cttcaggact tttctcacga atacaccgcg acgacaagcg
     7081 aatttaagga actcaaacgg gtctttccca aggaatattt cagcccgctg atagaaacgc
     7141 agatgaaccg ctgtgtccaa tgtttgcggt gcgtgcgcta ttgtgatcag gtcatggatg
     7201 tgaaagctct ggcgcctgca ggacgcggca ccatgacgga aatcaaagcc ttcggggctc
     7261 atgaattgga ctgcgagttt tgtggtggtt gtattcagat ttgtccggtg ggagccatta
     7321 cgagtcgact ctccatgtac gaatttcgcc catggatgct gaagcggacg gactcaattt
     7381 gtggctattg tggggatggg tgtcaaatca cctttcaaac gaagaataat gacctcatcg
     7441 aagtgaattc cactcacgga gctggccgta ataacggtga tctctgtgcc cgaggctact
     7501 ttggctacca tgtcagcgtt catcctgatc ggctcacggc gcctctcatc cgtcgaggag
     7561 aagcccttca atccgtgcaa tgggaagagg ccttggaact cgtggggcag cgtctattgg
     7621 acattaaagc caagtatggt cccgatgcga ttggcggatt aattacggga cgatgcacca
     7681 atgaagatat ctatgtgttt cagaaattca tgcgtggggt ggtggggacc aaccggattg
     7741 atagcagtgt gcggtatggc catctgaatg cggtgcatgc catgcagcgg gtgcaaggca
     7801 cccatcgttg ggccgtgagc tttgacgata ttctatcggc ggataccctt ttactcgtcg
     7861 ggaccaatat taccgaaacc aatcccatta cagggttgaa ggtcaaagag gcggtgaaac
     7921 gacgtggggc gaagctgatt acgatcgaag ccttgcaacc tgccattggg agcatcagca
     7981 atattgtgaa tctggcccac caccatttgg gcgtcaaacc gacgggctat gccggagcga
     8041 ttttgggcat gctgaaagcg gtcgtggatc agcaaatggt ggatccggaa cttcagacca
     8101 acgcctcgtc ctatgtcaac gacatgaccg atacgctgaa agccctgtcc tggaatgtga
     8161 ttcaagcgca aacaggattg gattcggaaa tgtttactca agccggccag gtgtttgcca
     8221 aaggggagaa ggttgtcatt ctcgtcggac cgggagtcct gcgatccgag ggtggattcg
     8281 gaatgaccat gaatttgttg gacctcttgt tgataagtgg aaaactcaca aaacccggtt
     8341 gtggtcttgc tcctctggcg gaagaaaata atgatcaggg agcctttgag atgggaggaa
     8401 tcggatcggt attgcctggc ggacaatccc tgggtagccc ggaggagagg aagcgagtgg
     8461 cgcaagcatg gggtcggcca attcccgaca cgccaggctc ttcgctccca cagatgatcg
     8521 aagatgcgca acaaggcaga ttgaaagctt tgtttgtcgt gggtgaaaat cccctggctt
     8581 cgttgcctcc gtcatcgggt attcagtcgg cattagagaa gttggagttt ctggtatgtc
     8641 aggaactgtt tctcactgaa accgcacaga aagctcatgt tgttctaccg gcctgttcct
     8701 atgctgaaaa agacggaacc tttaccaata cggaagggca tatccagtca gtacgaaaag
     8761 cgattgattt ggttggtgag agtcgaccgg actgggaagt gttctctgcg ctgtcggtga
     8821 tgatgaatga tccaatggaa tatggagacg tcaaggaaat cggcaaagaa atacataatc
     8881 ttctccctgg aacacgcacc ttagggcctg gtcccttacc ccctcgccct gacctgacgg
     8941 tcatcgcaaa atacctcacg tcggattatc aacgggatat ggcgacgcgg taccaattgc
     9001 cggcaaacga tttagctgat gatgcggatg acatgatctt gcaggtttcc caatccttgt
     9061 ttcattccgg aaagctttca cgaaaggcca aaggtttgct gcaggttgag gcaaccggca
     9121 aactttatct gcatccgaaa gacgcggctc gacgcgggat tggtgaaggg gacaccgtga
     9181 aggtgagcaa tcgtttgggg gaggtggtca ctcaggtcgg acttcgtgaa cgtattccca
     9241 acggtgttgc ggtgtaccct gagcattttg atgaagagat acgccgtctt atgccctata
     9301 gtgttgatcc ggaaacgcag gtgccctctt gcaaagtggc tcgtgttcgc cttgaaaaaa
     9361 tggctgggat atgaggcggt tcttagcttg tggtttgatt catagaggag aggagtgaat
     9421 ccgtcatgga tacgggaatt cgattagcct taacgttaag ccaaattggc gcagtcatgg
     9481 tggcagtgct tcttaccgtt gctgcgctta ccctgctgga acgcaaggtg ttgggttgga
     9541 tgcaggatcg gatgggaccg atggaggtcg ggccttatgg catcttgcaa ccggtagccg
     9601 acggcttaaa gctctttttt aaagaagata tcattcctgc cggtgccaat aagttcatgt
     9661 ttagcatggc gccagtgttg tcattagttc ccgccctcat cggattcgcg gtgattccat
     9721 ttggcccgga ttggacggtt gatgtcggcg gcgtcaactt taaaccgttt gtgattaccg
     9781 acatcaatat cggcgttctc tatatcctgg cgtttgcttc aatcggggca tacggcatta
     9841 ttctgggcgg atggtcatca aatagtaagt attcccttct gggaggtttg cggtctgcgg
     9901 cacaattgat cagttacgaa ttgaatgtgg ggctatcaat tgtcggcgta ttactccttt
     9961 caagctcttt gagtttggtg gccattacca atgcgcaggc aggagggttt tggaactggt
    10021 actttttcgg gggagggggc tttccccaag ccgtggcatt cgtcgtgttt attatttcct
    10081 cggtggcaga aacgaatcgg accccatttg atttaccgga agcggaaagc gagttggtgg
    10141 cgggattctt taccgaatat agcggaatgc ggttcgcctt cttctttctg gccgaatacg
    10201 gcaacatgat tctggtgtcg tgtgtggcca ccgtattgtt ttttggcgga tggcatgcac
    10261 cttttgagtt tttacaaggt ccggaatcca ttcattgggc tacaggtata ttttggtttg
    10321 tgtttaaagt ttcgagtttt ttatttttat tcttttggtt gcgcgccacg ttaccccgct
    10381 tacgctatga ccagcttatg cggttcgggt ggaaggtcct ccttcccatc gcgttggcaa
    10441 atattgtatt gacagctatc gtcgcatttc tctttccggg aagctgagaa ggttgaatgg
    10501 tatgaaactc aaggagtggg ttaaaacgct tttgttttat gagattgcgt tagggatgaa
    10561 ggcaacctta tcccatttac tccattataa gcccatcacc ttacaatacc cgcacgagaa
    10621 gaagcagttg ccgtccaact atcgcggcat gttggcgtta ctgcgttacg atgatggcac
    10681 ggaaaagtgc gtgggctgcg atttatgtga ggcggcttgt ccctcccgtg tcatccgtgt
    10741 agtgagtgct gaggtgccgg gggaaccgac taagcggtat tccaaggaat attacatgga
    10801 catgacccga tgtttgtttt gcgggttgtg tgtgaaggca tgtccggttg atgccttggg
    10861 aatgacacag gagtatgaat gggcggtcta tgataagcgg cagcttcaat taaataagca
    10921 acaactcctg gcaattggtg atcgaaatta tccgattcgg gaaaagcgcc tggaatttca
    10981 gcatcccaat gtggcctatt ttaacgtagg gtttaaggac attcctcaaa aagaatagta
    11041 tctgtttttg tgcggcgcta cattcggtct acatgccgga taaggaatat ccaaaaagtt
    11101 tttgaagagc agataatggg aacgatagtt tttttctatt ttgccagtgt gattgtggcg
    11161 acggccgctt tggtggtggc cttgagaagc ccggtatata gtgccttggc gctcctggtc
    11221 atgttctttc acgtcgcggg gctctttgtc acattgcatg cggaattcct ggcggtgatt
    11281 caaatcctgg tgtatgcggg cgccattctg gtgctctatc tttttgtggt tatgttgctc
    11341 aatttgaagg cggaagtacg ctttcaccac caattgaccg taggcctctt tctgggatgt
    11401 atgttgtttg cggaagcggt tctgctggtg gttaagggcg aaacctcatt gggtgcgaac
    11461 cttcctgctc cgggagagcc tacccattta acccagggca atacggaatc gatcggtgag
    11521 ttgctgtatt ccacgtatct gtttccattt gaaatcgcgt ctcttattct cttggtggcg
    11581 atggtgggtg cggtcatcct caccaaaaag gggatcttgg aggcgaagag ttaatggacg
    11641 ttccggtgag ttactatctt gtgctgagcg ggattgtgtt ctcaatcgga ttggtaggag
    11701 tgctgatccg acgcaatatc atcatcattt tattatccat tgagttgatg ttgaatgcca
    11761 cgaacattaa ttttgtggct ttctcttcct atttgggtaa tctttcaggt caggtgttcg
    11821 tgttttttgc gcttaccgtg gctgctgcgg aagttgcggt agggctggcg attatcatcg
    11881 cgctctatcg gcattcgacc agtactaata ttgacaactt ccggttgttg aaatggtaag
    11941 gcgatgctct acgccttaat tcctttcctc ccactcctgg cctttgtcat cctcgggcta
    12001 ttcggtcact ggatcaaaga ccgaagccat tgggtggccg ttcctgccgt cctgggatcg
    12061 ttcctacttt ccatcatggc gcttgtcgat gtggccggtg gtcaaacgct tcacatccct
    12121 ctctatacct gggcggattc cggaaatctg catattgcca tagggttatt tgttgaccag
    12181 ctcaccgtcg cgatgttgtt actggtcacc attgtgagtt ccctggtcca tgtctatacg
    12241 attggttaca tgcatggaga gccgggctac gccagatttt ttagcaatat cgcgctcttt
    12301 accttttcca tgttgatgct ggtgatgtcc gataatttcc ttcagctttt tgtcttttgg
    12361 gaagcggtgg gtctgtgctc ctacttgtta atcggccatt ggtatgagcg tgagtcagcc
    12421 agagccgcgg ccactaaggc ctttctggtg aatcgggtcg gggattttgg attcatgttg
    12481 ggtattctgc tggtctttgt caccttcggc agtctgcatt atcaggaggt gtttcctcag
    12541 ttggagcaaa aggctggtga tttggtcaat cttcttggtt cagtcggagg tcattgggaa
    12601 atatcagtga tgaccttgat ctgcctcttt ctctttatcg gggcagtcgg aaaatctgct
    12661 caagtgcctc tgcatgtgtg gttgcctgat gcgatggagg gtcccactcc gatttcagcg
    12721 ctcattcatg cggcgacgat ggtgacggca ggggtgttta tggttgccag atttgctccg
    12781 ttatttaacc tttctcccgt ggccatggat gtggtggcag tggtcggtgg agtcacgatg
    12841 tttattggag cgacgatcgc gttgacgcaa accgatatca aacgggttgt ggcgtattct
    12901 acgctcagcc aacttgggta catgatgatg gcctgtgggc tgggtggcta tattgccgga
    12961 atgtatcatc ttctgaccca cggggcgttt aaggccttgt tattcctcgg atgtggggcc
    13021 gtgatcattg ctcttcatca tgagcaagat atgaaaaata tgggtggact caaagataag
    13081 ctccctgtga cgtattggac ctttctcatt ggggccttgg ccctgtccgg ttttcccttc
    13141 actgcgggat ttttcagtaa agaccaatta ctcctggcgg catggatgtc cggcgacctg
    13201 gggaaagtcc tggccgcctt gggattactg accgcgctca tgacggcctt ttacactttc
    13261 cgattggtct ttgtgacatt ttggggagaa tcgcgagtgg atccccacca tgccaagcat
    13321 gtgcatgaac cctcaaaaac aatgacgatt cctcttatga ttcttgccgt tctgagtatt
    13381 ctcgcgggat acatgggaat tccagagttt ttggcccccg cgtttcccgg tgctgagggt
    13441 ggtggaggtc atcatgaagg atctgcggcc atgggcatga tggtgacggc caccgcaatg
    13501 ggtctgctgg gtattgccgg cgcctattgg gtctacgtga agtcgccggg tttgccggac
    13561 cgattggcca atcagtggca atcgctgtat cagttgtcct tgaataaatg gtttgtggat
    13621 gaagcctatg accggaccgt ggtgatgcca acattgaatc tcgcggaccg gttatggaaa
    13681 agagtggata ttgcggtgat tgatggtgca gtcaatggag tcgcgcgagc cgtggcctgg
    13741 ggaggatggg tgacgcgaag acttcaaagc ggacaagcac agaattatgc gctggccatg
    13801 actgtcggcg ctgtggtcat tttaggtgcg atgatctttt attagtttgg gtggcggacc
    13861 gtaggatggg atattcgtga tggttcaacc gacaaccgta tggcaggaga ttattcaatc
    13921 gtgactgaga tggtcattcc cacagggggg atcccctggc tcacgattgt gcttattctc
    13981 ccgctgctcg gagtggtcgc ggtcttgctg gctcaggaag ccctttcacg cactattgcg
    14041 ttgacggtgt ccatcctgac attggtggtc tccctgcctc tgtggttcgc gttcgacaat
    14101 gccagtgccg gcatgcaatt cgtggaaaag catctatgga tcaattctcc cgcgatccat
    14161 tattcgctgg gtgtggatgg gatcagcatg ccacttgtcc tgctcacgac ctttctcact
    14221 cccctctgta ttctggtctc ctggacgtcg gttcaaacca gaatgaaaga attcctgatc
    14281 agtttattgg tgatggagac ggcgacgatc ggcgtgtttg cggccttgga ttttgtgctg
    14341 ttttatgtgt tttgggagac gatgctgatt cccatgtatc ttttgattgg ggtctggggt
    14401 gggccgaatc gtgtctatgc ggcgatcaag tttttcctgt ataccctggc cggcagtatc
    14461 ctgctgctgg tagcgatttt agtgctgttt tttgaaggtg ggcgaacttt tgatattttg
    14521 gccctcacgc atgcgtccta tgcccccggg ttgcaatccc tgctcttctg ggcctttttt
    14581 gccgcctttg cggtgaaagt gcccatgttt ccctttcata cctggcttcc ggatgctcat
    14641 gttgaagcgc ccactgcagg gagcatcatt ctcgcgagtg tcctgctcaa gatggggacg
    14701 tatgggtttt tacgattttc attgcccatg ttgcctgatg cctcggttgc ctataccccc
    14761 gttatcatga ccctctcggt tattgccatt atttatgggg cctatatggc attagcgcaa
    14821 tccgacctca agaaacttat tgcctattcc agtgtcagtc acatgggatt tgtcaccttg
    14881 ggaatttttg cgttaaatgc ccagggaatt gaaggcgcgg ttcttcagat gatcaaccac
    14941 gggattacga ccggtgccct ctttatgtgt gtgggcatca tttacgaacg gacacatagc
    15001 cggcaaatta cggataatgg cgggttggcg aacgccatgc ctaaatacgc cacttttttt
    15061 gtgatttttg cgttgtcttc cgttggtctt ccgggcatga acagttttgt cggagagttt
    15121 ttagtcctac ttggcacctt tgcctggagc aagctcacag ggactcttgc cgcgttggga
    15181 attattttgg ctgccgtcta tattttgtat ttggtgcaac ggatgatcta tgggccggcc
    15241 tctccgaaga tgctcccaaa attaacagat ttaaatgttc gcgaatttgc catgctggtt
    15301 ccgttggtgg tgctggtgtt ttggatcggg ctctatccaa aaccgttgct ggatgtgatg
    15361 catgcaagcg tggcgcgggt catttcgtct caatccacct cggttctggt tcaacaggga
    15421 ggggatggcc caggtcatac cctggtgatg acggccgatc catccgggca ccctcttatc
    15481 atgaatggtg aggtcgcacc atgatgcttc ctctcgccga tattgtcctt attcttcctg
    15541 aaatgctcgt tgtgggcatg gcatgtttga ttctcgttct tgatcccatt acccctgcac
    15601 acaaaaaaga attcctggcg tggatgagtc tcggcattct cgtggtatgc agcgtggtga
    15661 cggcgagcgg ttttggccag cgagtgatgg cgtttagtga tttagtggtc gtggattcgt
    15721 atgccgtatt ttggaaaatg ttgctgtatc tcgtgagtgg tctgaccatt ttattgtcca
    15781 tggggtatct aaaagaggaa aagatccaac tcgcggaata ctatgccttt gtcctattgg
    15841 cattgaccgg catgatggtg atggtatcgg gggctgattt gctcacgatc tatcttggaa
    15901 tcgaactgat gtcgattacc ctctatatca tggccggatt caaacgtttt gaactcaggt
    15961 caatcgagtc ctctgccaaa tattttgtgt tgggggcttt ctcctccggg attttgctct
    16021 atgggatttc aattatattc ggggtcacgg gaagtacgag gctggtggaa attgcggccg
    16081 tggtcaatgg gcgcgggatt gatgatccgt tagtgtttat tgcgctcatg ctgttgatcg
    16141 tgggattcgg gtttaaagtt gcggcggtgc cctttcatat gtggacgccc gatgtgtacg
    16201 aaggagctcc aacatcggtg acggccttta tggcggtggc ttccaaggct gccagctttg
    16261 ccgcgttcct ccgggtgctc ctggaagctt tcggtggaat taaaccagat tggaatctgt
    16321 tgatcctggg catttgtatc atcaccgtgg ctcttggcaa tcttgtggct attgtgcaga
    16381 ccaacgtgaa acggatgctg gcctattcca gtattgccca tgcggggtat gccttgatcg
    16441 gggtcgtcgt cgccgggtgg agcggtacgg aggcggcagt ttcggctcaa ggcgtgtcga
    16501 gcttgatgct ctatctggcg atctattcgt ttatgacgat aggggcattt tccatggtgg
    16561 ctattttgcg gaaaggcggc ctcgaaggcg aagaaataga agatttcact ggattggcca
    16621 aacgacataa gggtggtgca tttctcatgt tggtatttat ggtgtcgttg gcgggtattc
    16681 ctcccacggc cgggtttatt gggaaattgt atctctttat ggctgccgtc aatgcggggt
    16741 tggcatggct ggcgatcatt ggtctgatct ttgcggccat ttcggctttt ttctatttgc
    16801 ggattgtcat ggtcatgtat atgcgtgagc catcctccga acaagaatta catacccgac
    16861 ttgttctttc tccccaggta tcgtttgtgt tggcctgcgc agttgccggc gtggtgttac
    16921 tcggaatctt tccaggtccg ctggtctcgg ttgcagacct ttcctcgctc cccattaaat
    16981 aaatatcggt tgtccttttc atgcccggcc ttttgggcta ttcatgggca tgaaagggtt
    17041 acgcggcttg tcccaatcct aatactccca ccccgatcat catcagcaca gcaccgacca
    17101 accgctgccc aacatatctt tccctcaaaa agagaatgcc ccatatcacc gcaaagagca
    17161 tgctggagcg cttgacggcg atcacggagg gaacaggacc aaggttgagc gcggtattat
    17221 ggaggagaag accgatgcct tgaaacaggc cgatgagtat aaggattccg acggtgcgcg
    17281 aatgagggat gccatggtta ggccgtggca ggaaacggag tcctaatata aatcccaatg
    17341 tcatcacact ggtaagggaa agactccaaa agagaggaga ggaattttgt acgccgatct
    17401 tgtcgaagtt ggatgtgaga ctatagatga aggccaccga cagcatgcgt ctggggcccg
    17461 gttgttggaa gatggcgacc aaaggaccca ataaacctcg gtgcaccgac tgaatgtgta
    17521 ggacatatgc tccagccaca atacaggcaa tgcccgcaat atcctggttc gttggcatct
    17581 ctcccaccag gagaggtgag gtgagtaata aaaaaagggg ggttaaggaa acgaagggaa
    17641 tggccaagga aagatcggag ctttgtaagg ccctcatgaa ttggaacatg gccaggatat
    17701 ttaaggtccc gcccaacagg agagcgggaa tatattgagg tcccagtggg ggaatggaat
    17761 cggtgagcag caacataccg agtagcaatg gaatgggagt cgcgcaagca gcgaatgcga
    17821 ccagttgagg tgagacggat cgtagtccct gcttactcaa taggtctttc agggattctc
    17881 cgagcgcagc caacagggca agagacatcc agctcatgtt ttgagcccca atacagacaa
    17941 tctccaggtg aaagaatcga tcactaattt tgtccgaata cactttggca gggacgcatt
    18001 cccaggatct tacaaggaat acctggatgc caccagcggt acacctgatg gcctaaggaa
    18061 gataaggaga cgaataggaa ctggaaaggg cggccgaatg gactttacag cgaagattca
    18121 agaagtagga tgattaggac agtgctggag aatttcctgg agggcaccat acagtcgact
    18181 gaaattggca gacgaggaac tttggagtgg ttgtgaggga agagagcatg tggctccacg
    18241 agacaacaga tagtgggtca gctcccgttg tccggagagg acggcagaat ggagcggcgt
    18301 caggccaatg gtattggtgg catcgatagg ggccccggaa tccagaagta ggcgagtcat
    18361 gtcggtatct cctgcctggg cggcgacgtg cagcgcagtc cagccttctt tgaaagaagt
    18421 ttgaacggtg gcgccgtgag cgaggagata tgacgccatg cgtcggtggt gttcctggac
    18481 ggcgatgtgg aggggtgtga tattcccttc aaggccatca ataagggcac cgttttgaag
    18541 gagaaggttg gcgatatcca catgaccccc cttcgcggcg aaatgcagtg gggtccattc
    18601 ggattgataa atagccccgg ggtctgcccc gttttgtaac aggagttggg caatcgtaaa
    18661 ttgtccggac cttgccgcga aatggagagg tgtggctcct tgtggcgtgc gctgattcac
    18721 atccgatcct tggtctatta accggtgtgt taaggtcacg tcccggtctt ggatggcttg
    18781 aataaggtca ggttgcgggg aggggctgtt ccactctgaa gcaaatgcat aggacagaag
    18841 aattccaagg ataatcccta catatttgac agtgatgact ccacgtgatt ggggcatgag
    18901 aaagcgttcc ttgttttgga ggtcagagcg tacggaataa gaaccgggct cactgagtat
    18961 aaagatgtag ggaacgtcaa aattggtggc ctacaccatt ccccatatga aggggagggt
    19021 cggcgtccta tttggtgagg cccacgggtt gcgtcaggaa tcaatcttgc cgaaaccccg
    19081 aacgattttt gttcatgttg gaatgaatgg tcgtaactgg accgggattt actggtggat
    19141 ggattcgtcg actgttttcc ccctttcccc cctctaaatc tgataagagg aaaaaagaaa
    19201 acatggccga ttttttggag aaataacccg gagtatcgtt tcggaatgtg tgaggaaatg
    19261 cctggggcgg agagaaaaaa gaggttaggt cttgccggag aaaagttttt ctttccggat
    19321 gatgaattct tgtgaagtca tgggttactt gtcgactagg tcatctgatt tttccaattc
    19381 atcggcaacc gacgctaata ggactttccg ttcccctttc gtcgggttcc ggctgaacga
    19441 gggcatgttg atcggcctga tgtatcagcc catccaaaac attgtgccaa tgggatgccg
    19501 tacccaagga tttttcatag agaatccccc cttctttggt ttccatattg agaaagctga
    19561 caacatggaa tggttcgtct tcatcgtgga tccacacaat gaggtcgatt cgctggttat
    19621 cttttggggg agaaggggtt tgccaacttt ttatcagtct ttccgcgttc acgtgcatga
    19681 gtttttgaaa tgagatgagt gcttttgggc ccgttcgtaa ggtttcttga aggacttctc
    19741 catttttcac taaaatcgaa ccgaccaggt ttgaaatagg aagcaatcgt ggggggactg
    19801 catgagaatg taataggcaa atggtgagag attggtcgtt cacggcaatg cctccaagcc
    19861 catcgcttcc catgaacatt tgtgatggaa tgaaattggt gccgaggatc cggttttctt
    19921 cttttttctt tttcgcgtga ggactcaggc ttaaggtgag cacaatgccc ccgagtgtaa
    19981 gagatacaaa cagcacaacg aatatcaggg tgaaatccat caatgaatcc tatgactgta
    20041 aggggcccta cggtgttatg gaaagtgcaa aacggcatat gaatcgggga tgtgggggaa
    20101 tttttgttgt catcttaaga cctgagttaa cctccaaggg cgtgaaccga gcagaatatg
    20161 cagcaatgcc ctgatactga ttgctcttct ttcgcttacc atgtgaatag aaattttggt
    20221 tacggaaacc aagccgagag gggttgttca agatgctgta tttggcatta tttcgacctg
    20281 gctcccaatg tattatttcg gcagagtcgg atgtttgctt tatggggcga tcaggaaata
    20341 atttgttgat ggaaacttac acggttactc atacgagttg tgtttggaag atgagggtgg
    20401 tgaaccacaa aacttgaatg tccgaaaagt agggcgtcga cggggttgca taagggaacg
    20461 agcgggaata ggtgctggta gcaagaactt cagaattcgt tgaagttccc gaggtcattt
    20521 gctaagctga ccttccgttt ttttacccat caacagagac gagtggcagg agaacacatg
    20581 gcaagattgg ggaaggcgct gatcagcgta tccgataaaa ccggtgtaga gaaaatggcc
    20641 aagggcctgg cggcacttga ggcagatatt ttgtcgacag gtggaaccgc caaaatgctc
    20701 agggacgcgg gagtggccgt gaccgaggtc gcagattaca ccgggtttcc tgaaatcctg
    20761 aacgggcggg tgaaaacgtt acatcccaag gttcatggtg ggttattagg gcggcggtcg
    20821 gtggatgcgc atgtgaagca aatgcaggaa caggggatcg agcccatcga tgtcgtagtc
    20881 gtgaatctct atccgtttga agcgaccatt acgaagcctg gttgtacatt tgaagaagcc
    20941 attgaaaata ttgatatcgg gggaccgtcc atgctccggt cggcggccaa gaatcatgag
    21001 gatgtgctgg tggtggtcga tccacaggat tatgagcggg tgttggaggc cttgcagtcc
    21061 gggaccgtct ccttaggatt gcgacgggag ttagccaaaa aagtctttga ccataccgcc
    21121 aggtatgaca gcctgatcgc caattatctc acctccaagt tagccgatac cgcagggcaa
    21181 aaatttccct cgttattatg cctgtcgtat gaaaaggtcg aagatttgcg gtacggggag
    21241 aatccgcatc aggccggtgc ggtctatcgt gatcggcaga ctcaagaagc ctctctttct
    21301 caggctcaac agcttcatgg caaagccatg tcctataata attatttgga tgccaatgcg
    21361 gcgttggaac tcgtgaagga atttgatgaa acggcggtgg tcatcgttaa acataataat
    21421 ccctgcgggg ttgcgatcgg ggatatgccg gtcgaggcct atgtccgggc gcgagagaca
    21481 gatccggtct cggcatttgg cggggtcatt gcctgtaatc gaaacgtcga tctggccatg
    21541 gccaaagaaa tcacctcgac gtttgtggaa gtattgattg cgccggcatt ttcggaagag
    21601 gccttggcgg aacttcaacg gaaaaaggat ttgcggctgt tagcggtggg ggcgttggag
    21661 agtgctcaac gggatgcgtt agatatgaaa aaaattgtgg gcgggatctt ggtgcaggat
    21721 cgggatctgg gatcgttgcg tgagatggga gacttatcca tgccgagtga acggcagccg
    21781 accgactacg agtatcaagc ctgcgatttt gcctggaaag tctgcaaaca cgtcaagtcc
    21841 aatgccattg tctttgccac gcagacccaa accgtgggga taggcgccgg tcaaatgagc
    21901 cgggtagatt cagtgaaact ggcgcaaatg aaagcgaagc ttcccatcaa agggtgcgtg
    21961 atggcttcgg atgcattttt cccatttcgt gatggcattg atgcagccgc agacgcaggc
    22021 attaccgccg tgattcaacc tggagggtct atccgggata aggagatcat ccaggccgtg
    22081 gatgagcata agatggccat gattatgacg ggtatgcgtc actttcgtca ttaaccgaaa
    22141 tcaatccgat tttcctttca atctttccgg attgcggttg ttcagcgtga tgccgggtgt
    22201 gttttcggat cggagaggtg taaccgggct tcgggtcatc agtagtagga tagggcacag
    22261 gccatgttgg cgcattcccc catcctcggg atggagcggg aagggaaagc aatccgtgtt
    22321 ccgatgaacg tattcatcat aggcaacggt ggacgagaac acgcgctcgc gtggaagctt
    22381 gcgcaatccc ctcgcgtgac caaactcttc tgtgcccccg gcaatgcggg gattgcacag
    22441 gtggccgaat gtgtgtcact tcaggtggac gaccttgaag gcctcaagca ctttgcccaa
    22501 acccacgaaa tcaatctgac gctggttgga ccggaactac cgctttcctt aggtatcgtc
    22561 gacgaattcc ggaaatccca catgcggatt tttggcccta cccgcaatgc ggccctcatt
    22621 gaatccagta aatcgtttgc caaagaactc atggtgcggg aaaaaattcc caccccatct
    22681 tcgcacacgt ttgacaaact ggagccggcg ttaacctatc tggaatcctg tccgctcccg
    22741 gtcgttgtga aagccgatgg tctggcgcaa gggaaagggg tcatcatctg tgcgaccagg
    22801 gccgaggccc gtgaggcggt ttgcagcatg ctggaagagc agcgctttgg aaaggcaggc
    22861 gcgcgggtgg tgctggaaga atttctagag ggagaagaac tcacggtcat ggcctttgcc
    22921 gatggccgaa cggtcattcc gatgctgcct gctcaggatc ataagcgaat cggtgagggg
    22981 gacacaggtc tcaataccgg cgggatgggg gcctatgcac cggcgccgtt gggaacaccg
    23041 gaactccggc agcgtattct tgatgaggtg ttgtgtccgg cggtcacagg gttgtcccgg
    23101 gtggggagtc cattttacgg cgtgttgtac gcggggatta tggtgaaaga tgggaacccg
    23161 tatgtgctgg aatttaacgc ccgctttggc gatcctgaaa cgcaggtcgt gttgccactc
    23221 ttaaaaacgg atttgcttga tgtgcttgaa gccgtggtcg aacatcgttt ggaccagttg
    23281 caggtagagt ggcatgatca ggcggcggtg tgtgtagtat tgacctccgg cggatatcct
    23341 gggtcctatc aaacgggtct cccgatcaca ggcctgccgg aaaaagagtc gaagtccgtc
    23401 atggtgtttc atgccgggac gactcatgcc aatgaaaaga ttgtcacgaa tgggggacgg
    23461 gtcttagggg tgaccgggct tgattcaacc ctggcgggag ccagagaaca tgccaatcag
    23521 gccctcgcct cgattcagtt tgaaggccgg tatttccgtt cggacattgg ccaccgcgtg
    23581 ctgcaagttc catcctgact caaagggccc ctattgtggc cacagttctc gaacttaatt
    23641 ccgcaccatc acctcaacag attcatcatc ttgcgcaatg tctgcaggat ggcggagtcg
    23701 tggctgtgcc gacggatagc ttttacgcgc tcgcggcgag tcccttcaat gagatggcat
    23761 tagagcgtct gatgcacatc aaaggggaaa gaaaccacaa accctttccg gtgttagtgg
    23821 gtgacccatc gcaactggac tggctgacgg aagtgattcc tgatgtcgct cgaatactca
    23881 tgaaagagtt ctggccgggt ttgctcacct tgatctttca ggctcgttca accttgtctc
    23941 ccattctgac cggcggccag ggaaccattg gtatccggca gccgaatgat ccacgagtct
    24001 gcgaactcat gacgcacatt ggaccgctta ccggcaccag cgccaatcga agcgggcagc
    24061 cgccggcaga atccgtggga gatgtgatgg aacaattagg ggcagtggtt gatgtgattg
    24121 ttgatggtgg ccctacaccg ggagggcaac cttccacggt gctacaaatc ctgccaaagc
    24181 cgcgcatcat tcgccagggt gctatttctc gcgatcgact ccaacagatt ctgtctgcta
    24241 gttctatcca actttcctaa ttaaaactaa agtgagccta atcctctccg ttccttttta
    24301 aggaatccaa ggagcggatt ccttcctaaa ccgatcaagc ttctggcagg gatctgttcc
    24361 gtttcagccg ttgaccaatt gacatatttc gcaaattgag taaggattcg gttggccaat
    24421 attagtgttt ccaatattgc actgcccctc cagtttgtaa gatgtgtatc aggcatttat
    24481 cttgagtttt accagtaaaa agacgagggt aagtgcatgc atcgtactta tgttcacaac
    24541 tttcgtatca acgtaatgtt ttatgttgcc gggactcgcc ccggctggcg aggccctttt
    24601 gtttcggcaa aaggtcccaa aaccagtgtc acccagatcg gcccatttta atcgtgctga
    24661 cgcaggtaac ggaggggcgg accaactcgc cgggctcaga caaggtccgc ccctgatttg
    24721 agcgtcagcc cgtttggccg atctgcaggt gagggaggaa aatgaaggtt gaggacaggt
    24781 gtagaaaaag gactgtcgtg gctaaggcgt ttggagtctg gcggagggtt tcacccccgc
    24841 ctgatgacaa aagctagata gactgctgca gccagctata cagaccaata tccttcaata
    24901 ggtagacgct atatacgtgt gtgagacgta atgggtttat agagttaact ggtcctcaat
    24961 atgccaagaa aacgtaacca aaagcgtcga aagcgcaagg caaaggtgcc caaggggcag
    25021 agaaagccaa gagagaaatg gattcatcta aaggccacag caaacgccga aggccgggtc
    25081 gagtttcggt tctctgacga gggtactctc atttgtccag aagcagaact cggttccgtg
    25141 gtgtcgtatc gtacctattc ccgagagtcg ggaaagccga aggtaattgc ccggtctccc
    25201 ggattgagct ttgaccttcg gaagaatatt gaactaagtt atgatgcaat cgctgggatc
    25261 gatacgaacg attttaactt tgatgggcgg cacctatcga tttgctctag cttttgttcc
    25321 aagccgatac tgaagaaccg tccagattct atatgggttg atattgctcc tgcttttatt
    25381 cttgaagggg tgcgagagaa tttcaatcct gaagtgatcg gttggcacct ctttctcacc
    25441 catgttctcc cactattagg tctttctgaa agccagcgtc ttgcattggt agtggacagt
    25501 gagttgagca atcatatccc aatgaaccag cgtcgaatgc cctactttcg aaatcatgat
    25561 ctgccaacct gcgttgacat actttatgcc tcatccgaca ctggagacta tttgccaaat
    25621 caactcatca agcaatgtga taaggcgtca cggttactgt tttcgcagat ttgtgatggt
    25681 gagattgctc tgcctcctat acttgtgggt ggtactacag aattcgctgg gtatgcatat
    25741 gttaacttcc tgatgactcc ataccagata gcgaaatcat ctgttggatt cctaggccgt
    25801 ggggtctaac ctatggttga agtcgctgaa ttgacggacc tccagcccgc cacccagcta
    25861 caaatttttt ggggggtgac tggtagcacg atgaatttac agtgcttacc gctcttagtc
    25921 ctaacatctt ggtagaacga ccatctcatg cattagagaa aattgtgact atgagttcct
    25981 ataaaccaag ccaaagtggt cttgccccct gtaaacgcga tgtaatcgat ctgaagtcag
    26041 ccgctgacgc tcttggcaag acgttggtga tgggaatgga gctatctttc gagcttcaag
    26101 ctattgcaca aaatccaaaa caccctttcc acgcaaatgc tgaggaaatg cctttacaga
    26161 ctttggtgct tcttcaagga atgctcatag ctgagcgatg gctcattgag tttcttctcg
    26221 gtcccagtct gagaggggac aaaaaacggg caactgacat ttgcgttgat tgtttcaatc
    26281 acgactggcg cttccccaaa ggaccggtag ccgatgagct tgtggcgcgc ctctctccga
    26341 tcaataaact agtacttcac ccgacttgga atctggtcaa cgcggctccc cggaaatgga
    26401 cactcaaccg gattgctcgc tgcatcgagg gactcgatat ctttgcagac ggccttcaag
    26461 agaaacgacc ggaggtagca aagatattta agagccatgt ccgagccgcg cattcagtta
    26521 tctcgcctgg tcccctgagt gatcgttttt gctggttcca tcctggcact cgcatggtac
    26581 ctactgctgt tacctcgcca aatgatattg gagctaggca tgaagtcaga ggcgtccaac
    26641 gggatggtga gccgacttcg taagctcgat gactctccct caatgttcag ccgaatgcag
    26701 gcggaggacg ggagattcag gagatcgaga ttagggggca agtctgttgt taagagtttc
    26761 aggtaaggga tcaggagcct gtttttggaa actctgtttc ttgttcgacc atataggggc
    26821 agtctgttgt taggtagggt atcaggaacc tatctttgtg tactcttttc cctgagttcg
    26881 tccataaggt cggggttcca tttgcttggc ttatggccga tatcaggact tgccataaga
    26941 tattccggaa gtatggccgg tccgccactc agttgcggac tctgttaggc tatcaccgga
    27001 taggacaccg ccaatgaaga ttgtcgaatt tgcttttatt gtatatccgg ccacggatct
    27061 ggcgcgatcc cgtgcctttt atgagggcgt tcttgggctt accagcgcaa cttccatgga
    27121 tctggtcgac ggattctggg ttgagtatga gatcggtcct catacactcg ccatcggaaa
    27181 agaaccattc ctgaagcctt cgggcgacgg tcctcagctc gtgctggaag tgaacgattt
    27241 tgacaagacg gtagaacatc tcaggtatca caacgtgcag ttcgcccttg agcctttcga
    27301 ccttcctcac tgccgggcag cgattgtcat ggaccccgac ggtaacaaac tcggcattca
    27361 taaacgaaac tcctaaccaa cgagctgcag catggggacg attccatttc tccttgtgac
    27421 atttccattc tccgcaatgt tctgtggttt ttttgtcgtt tgggggagct gggtttcggt
    27481 ccggcagcgg aggccttctt gcataatgca tctcctagag cgcgaccttt gtttggcaca
    27541 aggatccaaa gctctttcca cgcaggtcgg tcccattcaa tcctccctct tttcgcaatt
    27601 cactgtattc ctcaaagcct ctatggtcgt ttaattactt caagggtatt tttgcagttc
    27661 acctgctgac agtatgtatg ctgatttggg gattcccctt ttttgagatg gcttggaaaa
    27721 ccacaatgcg aatcggcttc tttcatctgc cctttgggta tttatacgcc tagtgttcac
    27781 aagataggca aaggtttgtt tcagcagccc agaaaagaat tgggaaggga gaaacgctat
    27841 aaatattcca gaaaacagaa atgttccatt aaaaaacttc tggctgagtt tgcgcagcta
    27901 gcgaatggaa ggcataatgc gatgaatgct gcctacacga gaaagaaaaa agctcacgat
    27961 gaagcgaaga atattttcag ttgaatctca gtacgggagg tatgcttatt tgtagaagtg
    28021 agatttctta gcgagggatc tgacccatgt gtttggtatt tgttgaggcc cgctcaaaca
    28081 ggcccgttca cattcctgct ctcccgtacc tgactataaa tctgaattca tagaaaggga
    28141 cgctattcat gcatgagttt gcacctgttc atcctcacca tcatcgtcat gtggccaaag
    28201 gcttccggct ttcacgattt tccgtgttgt tgacttcatt cctgatgctg ctaattaact
    28261 cgttgctggc gaccagtgcg gtattgccgt cgacggttgc cgcacagtcc gctcccggcg
    28321 aacttgatag caaagctctc tacgattcct cagccggtcc ggtatttacc ctgccgccgt
    28381 ggaccgatgc ctctggctgg gaagtccgcg aaaactacgc gaccattcag ttggctgata
    28441 tcaacggtga tggacatgac gaacttctcg ggcgtggcaa caacggaatg tccgttcagg
    28501 agtgggacac caccctggga ctctggaacg aaatatccgg cagcggccca ttcaccaaca
    28561 aacagggttg ggatcacccg cagttcgctt taacgattca gacggcagat atcgatggag
    28621 acggcgcggc tgagctgctt ggccgcagtg cgaccggtat ggagaccttc aaatgggatg
    28681 ggacgaattg gaaactgctc aaagcagagg atccatactg ggctgataaa aatggttggg
    28741 atgccgctaa atactactca accatccaga ccgctgatat tgatggtgac ggcgccgacg
    28801 aacttctggc tcgcggcgtc aatggcatgg atacctacaa atggaccgat ggcgagtgga
    28861 agaacctcaa aagcggtagc ccaccctggt cggatgctgc cggttgggac aaggtccagt
    28921 actactcaac gatccagact gctgatatcg atggtgacgg cgcggaagag ctgctggtgc
    28981 gcgccgcggg tggcatggac acctacaagt ggaccggtag cgaatggaag tacctcaaag
    29041 gcggtagccc gccctggttg gatgcctacg actgggatga ctccgtggcc tacagaacca
    29101 tccagaccgg cgatctggac ggcgatggca aagccgagct tattgcgcgc tcggtagact
    29161 ggctggaagt ctatacctgg accgatggcg agtggaagac cttctttaat ttccttgcgc
    29221 atacgtactg gcccacctac caggggtgga ataatccagg gtactacctg actatccagt
    29281 cggccgacat tgacggagaa aaaggcgatg aacttctgat tcggaatgcc tctggtatgt
    29341 tggcctataa gtggaaccca acgtcgaagt tattggatcg cattgtgtcc gacaacccca
    29401 agctggccga cgattactgg gccgatggtg acaattacct gaccatccag acgggggatg
    29461 tcgacggcga tggcgctgcc gacctgattg cccgcggagt ctacggcata cgcacttggt
    29521 cctacaataa agtgaccgcc aaggcctggg actatccagc caagtatggg ttgaaagttt
    29581 tcaccggcga tgagactatc gcctacgact atatcaacag ctatctgact cttgctgccg
    29641 gcctgaccat ccgcggacag tacacgacgg acgcaaatac gttgaagggt taccacacgt
    29701 gcctcaacca ccgcgtcagc ggttataact ggaccgactg ggcatcactg ccaatcgaga
    29761 cctgtggcgt acccggtacc ccgatcacgc caccatccgg tctgactctg gttacctgga
    29821 acaaggtcgc cgagcagatc tggaacgaac tagtgctggc gcaggcggtc tccgactact
    29881 acgcggatct tctggcgcta tacaacacta ttttcaataa tgagcagagc atcctgactg
    29941 ccacagcggg aactctctat aacgagagca tggacgagaa gagcgtggac gcgacattcg
    30001 aggatctctt aattgcgccg tttgcgctct cgtctctggg tggcgatgtc gcggcggctg
    30061 taggcggaat tctttccgcg ctcatcgtgg ctggtgtgga cgcatccggc gatgacaact
    30121 tccagggtac atggagcgac gctcagcacg agttaactgc aattttgggt aagtatcaac
    30181 atattatccc tgctggcaag gcttttgtct caggcgactc gggattcttg cagtacgtgg
    30241 cagagcagaa aacgtccgga gcctgggatc cgtcgaatgc gtgggtgacg cgatcgatgc
    30301 tcagtgaagg ccagcggcag atagcacgct ggatttacca gacactctcg ccatcggtct
    30361 ggaagatgta tgcagcctac ttcaccatgt caccctgtga gcggccccct gatagcatcg
    30421 tctactacta tgttgatgac aacgctcacc aagactgttt ccggcaaatc gtcggagccg
    30481 aagattccca actgcacaac attatggatc cggtctcccc atcctgtgcg ccaaccaagg
    30541 aaaatctcgc aacctgggaa tatggtagct gtagcatcgg cgaagataaa gctaattttt
    30601 tcctgaaccg tgacggctgg aagtttaggg ttaaggctgt cccggcgcca tagtgagcaa
    30661 attctcagat gccaccggca cttgggtact gaggaataat tcattacatc tagtggcacc
    30721 tatgagaagt cctgttaaca gggcaagatc ctccgttctc ggcatttttt gttttttgcg
    30781 tccgctctcc gttcctttac tgtaaatcta tggtggaaag tttgcggaca ggtatgagat
    30841 caggagccta cctttggtta gtctttttcc tagggtcgac catgttacgg aagtttattc
    30901 gggttggctt agcgtcgcgt atagttttgg ccatacgtcg acgttttcaa tctaactgga
    30961 aaaagcgaac tggattaata ctggagttca gcctgacctt aagaatcatt ggtcccggca
    31021 ataaataggg cacagaagct gggtggtgat cctttcgaac gtaaagctta gttgtaacag
    31081 ttgatcctga attagaatga cttcccggca cacttcacac ccgtgagaga gttggacgca
    31141 ccgggtggag aggcgtccac ccaggtgtgt catgttgctc ggttttctcg gcccattcga
    31201 aatacatctg cctcttatcg gttccgtaag attcgaaatg ccattggcgt ctcaatcgtt
    31261 ttgattgcac cgttagtgcg aggggctttt actctttctc tatgaaagac acatcggtga
    31321 ccttattttc ctcatcaaca aataatacga cttcttgtcc tgaagaaaga tttttaaact
    31381 tatcttgaat gatggaacgc acttcatact gttgttcttt tttcccatcc tgttgaacgg
    31441 aaaccttatt gtctttcagt ggttctttca ggatgcctga aacctgtttg aaatttcctt
    31501 tcaggggaga tttctttttc tcagggtttt tgattttttc cacttctgcc gcattctcaa
    31561 atgtgacgtc aacgatctga tccatttcat caatgaggaa tatagcttcc gcgccaaccg
    31621 ggatggatgc gactttgctc ctggccaatg gcctgatagg atgcgtttcc tcttcacctt
    31681 ccttcgactg aatgacagct ttttcatgtc cggtcgccag tggctgagtc aacaccccat
    31741 gtacggttcg gtgattggac tcttcacctt ccagatgaac gtccacaatg aggttttgat
    31801 cgttaacggt tatgtcaact cgatcaccct tttttaaagc gggaagccct ttctcctcac
    31861 ggagattcat gggtagatag cgaggttgag cttccccggt atctaccttg gcttgttccc
    31921 ctctgatttc ttccacagtt ccactaatga ctcgttcccc ggacagggtt ccgctttctt
    31981 tcgtatcttt tccatcatgg gctaacgcca caccggaaaa aatcagacat ccagtcaaca
    32041 agcacattcc atacagtttt aagcgtccga tggcgccctg tgcggttgga aactttgatg
    32101 tgtgttgaaa taccgtcgtg aaagacatgg agcactcctt tggctaattg aacattgaga
    32161 gatttgattc agacatttga aatatatcca atttttcttc aggggagacc tgaggaaaac
    32221 cctgggtctg atattcatag attttctatg gagtgggaaa agggctggaa gaactcaagg
    32281 cggggattct gcgctatgaa gtccgttttc agttggcgtc cgggtggatt tcgataaaag
    32341 aagtaagccg atacaggctg agaggcagga ggcgattaaa atggccaatt tcgctgtttg
    32401 taaatcggag ggttcctgga aagccaaggt tgcaatgaaa atggccatgg taaatccaat
    32461 tcctcccata aaacccaaac caatgatttg tggccagcca agatcggatg gaagaacagc
    32521 actgccaaac tgaacgacaa cccaggctcc aagacatatt cccaatggct tgcccaacaa
    32581 caatcccagc ccgattccca atgagacgga atggctgaga agggaggaga agtccgtggc
    32641 aatcaccacg cctgcattgg ccaaagcaaa aattggcatg atggcgaacg tgacccacgg
    32701 gtataaggca tgttcgagtt tttggagtgg gggttgaatt aaatcctttg ccgtttcgag
    32761 agccttaatc gcctcagact gttcttcatt ccctaacaca ggaacccccg agacttcggc
    32821 ttttcggaat tcttgactga atttttcgct tttatgggca aattcctttt tcgtgatagc
    32881 ggaagtggcc ggaatggtga aggcggcaag cactcccgca atcgttgggt gaaggccgga
    32941 gaaaaggaac gccaaccaca ggccgccaat cccgaatagg ccatatataa agggagagcg
    33001 aaggcccatc cggttggcac tcataagtat ggctaagaat acgcaggcgg ttccaaggct
    33061 gatccaggag atttccgacg tataaaacaa cgcaatcacc agaacggctc caagatcgtc
    33121 aacgatagcc agggtggtca gaaaaatttt agcggaaaag ggaatccttg aacccaatag
    33181 gcccagtagg ccaatggcaa aagcaatatc ggtggccatg ggaatgcccc acccggaact
    33241 gtagggcgat tggaaattga acaggagaaa tatgaatgcc ggaatcacca ttccccccaa
    33301 agcagccgca atgggaagaa tggcttgacg aggttcggtt aattccccca ccaatatttc
    33361 ccgtttgatt tcgagtccga tgacgaaaaa gaaaatggcc atcaagccgt cattgatcca
    33421 aagtattaaa ggtttctcta atagatgggt gccaatgcca atggtaaaag ggagttccca
    33481 aaaattcgaa tagagatcgc caaaggccga attggcccaa agcaatccga tagcagtaca
    33541 gaccaagaga acgattccac cggaagcatt gagtttggtg aaatcctcaa agggcgaaat
    33601 aacccgttcc atcggtcggg ttatcacggt ttgtttcgcc tcttttacaa gatccatgat
    33661 ttcgaaaatt ttggattatg acgtaaggtt cgggatccac ccatggagga gatgaatgcc
    33721 tgtctgcctg ccagttagtg aagagttgtt aagagatgct tgcataattt tgagtgcgaa
    33781 ggtcgaaccg attcggctgt tatgggtggc actgtccaaa agctggtggg gccacgaatc
    33841 aacccggatg acacaatctg acgataaaga actccctaaa atgaagtgtt attgatgata
    33901 catgggagag aatttgaaat ctaagacaaa tgttaaagac aggagtctgt tttgtctctt
    33961 gaggtccatc cactcccatc cattaggata aaggaaatta gaccatagga gagaaaatga
    34021 aatgaatttt tcctaacaac ttcccaaatc ttatgggaga cgaacctttt tgaaggcaaa
    34081 tcaaactttt ctcggccttg ctgtggtgat gggattgttg gtgggtgcca gtctatgacg
    34141 ggtcaagcga attgatactg atctgacagt tcatcttcaa tagttcaagc cttcattgtt
    34201 cccggaaact cccgccttag tggcgggaga tatttttggg tgaacggaat cgtcagagga
    34261 ttaatcaaca gcaaaagcca gtattttttt catatagatt gaaaaatatt ttctcattct
    34321 gcaagttccc ccatggaagg ggtggccacg gctggaatca cacatggtaa cccaggaaaa
    34381 caaatcatgg atgattcgta cccttatcat ttcattaggg acgctgggat ttgccattct
    34441 ggtggtgtgt ttggcgtcat tttttgtcga tgaacctcta cgaaggtata tcgaaacaaa
    34501 cctcaataaa aacctcgaag gatatacggt gcaattagag gcagtggatt ttcatccatt
    34561 ggggttttcg atcgactttg aaaatttaac gttaagacag gaaaccaatc ctgatccacc
    34621 tcttctcatc attccctcct ggactgccag tattcaatgg acagaattaa tcaaaatggg
    34681 aattgtgagt gatcatgtca ttagaaatgc tgaagtaaaa ttccttttca ctcaagccga
    34741 aaaagaagtg aaggataaaa caggcgttga agacaaaggt tggcaagaag ccttatatgc
    34801 gatgtacccc gtcaccatca acacttttcg aattgaagat agttcctttt catatcggga
    34861 tcagcctgat caacctccgc ttgaactgac caatctccta ctggacgtgg gaaacataca
    34921 gaacgtccgc tcacaagtag gggagtatcc atccagcatt catttggaag gatccctgaa
    34981 tcagaccggt tttgtgagta tctcaggaag agccaatttc ctagccgaac cgtttccagg
    35041 catatcagta gatttcgatt taaaaaatct tcagctgcag ccgtttattc ctgtctcctc
    35101 cctcgtcaat gtggaaatac attccggaac gctgaacgga cagggacatg tcgaatattc
    35161 cccttgggca aacatggctc aaattgtcag gctggaaatt gagaatccag atattaacta
    35221 tgtggagaaa cattctccca ggaaagcaca aaaggccaag caggagtccc ctacgcaaga
    35281 cacggatgat tcaaaaaaat cccaagaccc cttcaaggtg gcgattgact ccgcattagt
    35341 gaaaaatggc gaatttggct atacgaacca gacaacaaat cccccctata agattttttt
    35401 caatcatgtg gatctccagg taactggagt ggggtctcca aggattacac agaaaggaga
    35461 gttgaaactt tccgggaaat ttatgggaaa ggggaagacg tcaatcgaag gcggattccg
    35521 gccggaggtg aaacatcctg attttgatgt aaaagtgaaa attgacaaaa cagaaatgac
    35581 aaccttaaac gatgtattga aagcctatgg aggtttcgat gtaaagagcg gggaattttc
    35641 gttagtttct gaattgcata ctgatagtgg ccgggtccag gggtatatta agccattttt
    35701 cgataaacct gaaatttacg atctttcaca agacaaaacc gacaatatct ttcaaaaggc
    35761 atatgagggc gttgtgaatg cggttgcttc tctattggaa aatgttccaa gagatcaggt
    35821 ggccacgaaa acagaaatca gcgggaattt agaaaatatt aaggtaggga catgggagct
    35881 tatatggaat ctattcaaga atgcatttgt tgatgccatg aaaccggcct tcgaaaattt
    35941 aaaaaccact ggcaggaaaa attaagccga tttatgtgaa ccagggttgg aagcattcat
    36001 gtatgaaatt taaatgcgtt agattcggcc atgccagagg aggctgggag tcttgttggt
    36061 gcgaagaaca attctcacgc ccgctgttac aagaatatct ttcccatggt tttttaaaca
    36121 caaaggaggc ggaaaaatga acgaaaagaa ttatgtgggg gcagtgcttt tgatcgcatt
    36181 attagcgatg accggatgta atactatgga gggagccggc gaggatgtac aaaaagccgg
    36241 ggaagaaatt gagaaagcag cggattaatc ttccgtgtcc ataacataag gcggaattcc
    36301 tttacccgcg tttggggatt ccgtcttcct ctctctgttc attctttttc cctccaaagt
    36361 ccaacaccct gagtaaatca tgcctgtgaa tttgtccggg atacctttac atagcccctg
    36421 agcaacattc ggaaaacgaa ccggcttggc cctgtattaa gtctgaagga aaatctggat
    36481 aaaaaagagt gagtagaaaa ccaatttata cgttggtgaa gcggggtttg tcttgataat
    36541 acatagtaac gaaataatta agaagtgggt tacccaccca caggacattt aaaaacgttt
    36601 aggaatccca tcaaaggagg tggatgcaca aacgaagaaa aaggttttaa ttggtatttg
    36661 aaagattttc atattcacaa caacatttca aggagaacaa aatgaacaaa gaacaattca
    36721 aaggaaattg gaaccagttt aaaggcgagg tgaaaaaaaa gtggggaaag tttacggacg
    36781 atgaattaac ccaaatcgag ggagattacg ataaatttaa gggaagagcc caagaattat
    36841 atggagatca aaaagaggaa ttgaataagt ggactgagga ttggcaggcg aaaaaccaaa
    36901 cagttcgcta atcctatctt cactcctcgt gtaagatcag aatacccttt accccaggag
    36961 acgggagatt ataccttccg ctcctgggta aaggattgaa tgttttgggt gatatgaagt
    37021 gcctcaaata tctatcttaa caatcttcta ttccaacaag atttagtcaa gatgcatgga
    37081 ggaaggagta aaattatgaa catcacgcaa catatactga aaaacaaaat tgcctcaacc
    37141 gtggcggccg ttcttctcat gagtgctcca gcttgggggg caaacggaat gaattattct
    37201 gtctccgcgg aacaaggagt tcaaaacggc tatatccaca aggtgtataa tgaggaccaa
    37261 tttaatggca attggaagca gctaaagggg gcgctcaagg aaaactgggg agagtttact
    37321 gaggacgatt tgttggcgat tgagggccga actgaccgtt tcgaaggaaa acttcaagaa
    37381 cgatatggag accggaaaga agaagtgaag gcttgggtcg atgactggat ggaaaaacat
    37441 ccttatgaga agacccagga tcgacaacat taagacaaaa gttacttttc tgcgttcaat
    37501 acttccggcg aaatgggatt tcctcccatt tcgccgatat cccgggctgt ctatcacctc
    37561 ccatagctcc tataaaggtt ttggcatttt tcgtatcgct atgggtagat tatcagccga
    37621 atagcgaatt tcggcttgat ccccaacggt aattgactca tcgaccagcg tcatggagtt
    37681 cacttcaacg gaaatttccc tttcatcctg gtttttaata acataggttt tcccttcaat
    37741 cgataccact tcaccggtaa tatttcctgg gatgccgggt gtgagtccct cctgttggga
    37801 aacggtcggc atgtcggatt caatcggcgg ttcaattgcc acggttcccg gaagattatt
    37861 tgattgagca gggggtgtat tgacgggctc atgttcttcc tgacacgcaa taactccaga
    37921 aagtaaaaat ggcactaacc aaactgttct tatgaaaact gatcgttgag ttcgtgagtc
    37981 atgtttcatg ggatcacccc tattgtaaaa ttagaaaaat tactgtgcga gctcggtcct
    38041 tccttgtaat gatgatttta gaaaaagaaa attgaagtgt tgttcacaaa aaatttgatt
    38101 cggtggggcc ttctgccgga tcaatggctc tggacataat gctattatat ttctttccct
    38161 ccttttcacc tcaatagcat tcattttagg gcatggggag atattgggaa ctcatcaaaa
    38221 ccctggtttg gtttttgacg ttaaggactt ggcgggaatt tctatggttg ctttaaattg
    38281 aaaaggtaca tccacgattg tggatgccgt cgtgggggag ggggatcgtg gagaataaat
    38341 ctcgtgaaac ctgatataga ccgtttgtcg ggggatgagg tattgatgga ccaaaagcac
    38401 atcgggtcca tttcttcgtg gtaaaaaaat ggattcaaaa catgatgaat gatgcgtcga
    38461 ggtttgaccg aaatatctct acctgagctc tcgttgttat ggagtcgacc aagacctgat
    38521 cggcaccgta gatatggaat tttttggtga gccttcgatc actttatcac tgtatacgag
    38581 ataaatgaag gtttgacgtt tcttgtcaaa aaatctgacc acctgcaatt ttttaaagat
    38641 taatgagcga ctctcgtgaa acactttctc tccttcttca agctcatcaa ttatgcgaat
    38701 gggaccgacc tgtcggcagg caattgaggc atctgaagtg tcctcagcga caccaaccat
    38761 gcctttcaag ccccctgtct tcgctcgact cagatggcag gtgactcctt caatcttggg
    38821 atcatcaaac gcttcaatca caattttgtg atcaggaccc aaaaaattga atttggtatc
    38881 cacacttcca atttcctcag cataggcacc agaaaccagt gatatagaaa agatcaaaga
    38941 catccatgca acattgggat tcatttatta tctcctctga gcgttatttg tatagttgat
    39001 aggacaactt gtatgaattt gcactctacg ctatttggga ccataaacaa cacaaccaaa
    39061 acattgaaac cttcatcgag aagttccagt ttttttattg ataaggcgag ctatggtgtg
    39121 gatttggttg cagccctcaa tgaggcgtct ttatctaccc tgaaagccca ccccggctcc
    39181 ttattcatgg cccatgcccg ttccgtctat tgagacgaac actaccagag gtgttaccgg
    39241 gccttggtat tatgagggga aggctctggc gttgagtaga attcccctat cattaatttc
    39301 cacttttcgt ccttcatgtc gggccaatca tttttattaa agcctggcgc cgtttctaat
    39361 tttttcgatt gaatatccag agtcaaatgt tcctggcctg gtggatgtgc aagcgatgcc
    39421 catgggacgg caaagagctt gtcccccata cctaaaaatc ctccaaagga taaaaccgca
    39481 tatccgactt ttccgtcact ccctataacc agttcttcaa tcagcccaat gtcttcacct
    39541 tggagatttt tgacggattt cccaacttgc tcactggcct tgtaggaatc ctggtctaaa
    39601 tttgcagata aagcttggga aaggggtgcg acgagcaaga taaaacccca aaaaataaat
    39661 agaagcgaaa ccggcattca gacctccatt ctgtttttgt gtcgtccggg caattgggca
    39721 tcgaaatctt cttatgcagg cggcagcgtg cccccttcaa taaagagcca taaatgattc
    39781 tctaacatag atgaaatagg gcggtctatc aggatgttgt ccgctcctca ttcttgaggt
    39841 atttccccgg atactcaggg ataggataaa gcccagggaa gatttaataa gttacccatt
    39901 caagggtttt tgatagtggc cgatccccta ggaatccgct aaaagttacc agcctgattc
    39961 tatctatggt ggagggggcc tacatgattt atcttgtgat agaagatcaa aaaatttcag
    40021 aaatcattgc agagcgtttg cggaaaatgg gtaaaaagac gagggtaatt tcccaatcgg
    40081 aggaattgtg gatgggcatt cgggaaagtt ctcccgatat cattattctg gatggtaata
    40141 gcccgcatat taatgctctg gaaattttga aggatcttaa agcccaggaa tataaaggcc
    40201 agactatagt tttaggaggt gaatcttctg ccccgtttac ccctgaaatc tcccgtttcg
    40261 gtgctattca gacagccggt cgccctctgg cggccagtcg cgtaatgggc gctatcagga
    40321 ttgcccaaga acatttgaat gcggatctat ggcgttcctc gtcaagcaga tctgaagtga
    40381 tgagggattc cggccaaaag gcaacctttt tatagaccct aggactgaaa agtttgaaaa
    40441 tattcatgct gaaaaattcg gggtaatccc aaggaggaaa ttgttatgcc aaacaccaat
    40501 gcatcatttt cggcattttc ccgtgtagat caattgaaac agcaactgga aaagctagag
    40561 aatctcagtc agaccgaggt tgatgccaaa gaattggaca attttgatcg tgagactgaa
    40621 aaaattttga agcgtgtgga tggaagagat accaaaatgg ccgcctatag ttttgccacc
    40681 atggcagagg cagaagcttt ggtcaatctt cctgagtccg ctcaagaacc gacctcccgt
    40741 gaccttttgc aaaagtctct acagcagaga cgacaagtgt tgttgggaat ggttagtgaa
    40801 atggaaggca tggaggaagc ggaagcggaa gtgttaacgg gagaagataa ggaagatccc
    40861 ccgctcatgg gataaatccc tagaagaacc tgagcccgaa gcacgttgtt tcattgaggg
    40921 cggctgggcc tccgtgatgt tcctgtcggt cacgattgaa taggctgatt cacacctgga
    40981 tttttttgaa cacacgaaaa aggtacagaa gtgagggtaa gaccacaatc attcctgcca
    41041 ccaatgcgaa gagaagcaat tccagcgttg aagccggggc cgtggtgttg aaaatggtta
    41101 aatcgggttt tatgagatat ggatattgac caaatcccca tccccagata atagcggtga
    41161 catacccgac ggctgatatc cgggcgaggt gaaatcgttt ggtccacagg ccaattatac
    41221 ttgccagcat ccctaataaa gccagaatga gaaataccca tccggttgga ctttgtagca
    41281 atccttggct gatttctgaa gcgtaacttt tcgtaaggag gagggtcagg atagccatta
    41341 tggctgaagc cgcaagcgac cacagagctt ttcggcgata cagacctctg agagctggta
    41401 acggggtttc atggattaag taggttgatg ccaaaaatgc aaaaagcgtg atcgtccaga
    41461 ttcccactgc caacgggaaa ggttgaatcc aggggagcac aaacacgtca acgaatcctc
    41521 cttgaacgtt ctgcaggtag ctggaggtta gcgtgccaag aataatgcct aagaacatcg
    41581 ccgtgatcag actggagagc ccgaagagcg ttccccagaa tcgatggagc ggagactgct
    41641 ggacatcata actacggaag gcaaaggcag atcctcgaag cacgataccg gtgaggagca
    41701 aggtcaaggg aatgtgaagt acggtcgata ttctggcata ggcaagggga aaggctgaaa
    41761 agagaagcgt gatgaccagg atcaaccata cgtggttcgc ttcccaaatg ggcccaatag
    41821 cagtagaaat caagtgacgt tgcgtttgcg cctccggccc tctggccagg agataccaaa
    41881 tgccggctcc ataatctgct ccgcccaaca gggcatacaa ggtgagcgat atgacaatgc
    41941 acccggcgat aatttcctca agccctggca tgagtcttcc ttgaagatgg accatccggt
    42001 tctccaatct gtggaaatac cgtaagatgg cgatgtaaaa gccacagaac aatttcagcc
    42061 aatcccagat acagcagggt aaaaaaaata agcgaaacgg tcaggccggt catcggggta
    42121 acggcatcag tggtgcgcat aattcctacg atgatccaag gctgccgacc gacttccgtc
    42181 accacccaac cggattcgat ggcgattaac cctaaaggag cgcaacccac taggcatcga
    42241 agaaaccaac gggatttcca tagaaatttc tgtttccata tcttgcagaa tccccacaca
    42301 ctcatggcca tgaggataaa tccagatgcg atcatgattt ggaatgcgag atggaccact
    42361 acgagcggag gccaatcctg gcgaggaaaa tccaacaatc ctgtgacagt tgccatagga
    42421 tccccatgaa gtaagaggct gagcgcataa ggaatttcaa ttccatatga aatcgttgcg
    42481 gtggagccat cgggaatgcc tccaatccaa agggaagcgc cggaggtggt atgaaaatgt
    42541 ccttcgagcg ccgccagttt aatcgggctg taggtggcca ctgcgcgggc gagcaagtct
    42601 ccactgagag gttgaaagat ggccgccact ccccctgtca tcagggcaat tctgagcgcc
    42661 tcctggtgaa agggattttc cggttgccga agtaagaaaa aagcatgcac acccgccaca
    42721 aggaatccga ttgccacaaa tgccgccaga atcatgtgat gcgattcatg tagcccgatc
    42781 ggattggtaa aggtggcaag cggggcaatc tggataggct gtccatcgag tagctgaaac
    42841 ccgacagggt ggttcatcca accattggcc agcacgacaa aaaaagcgga tagaacacca
    42901 ctcaccgtca cgatcactcc ggacatcaaa tgcagataag ccggaatctt cttccaccca
    42961 tagagataaa ttcccaaaaa gatagcttcc gtgaaaaaag caaacccttc taaggaaaac
    43021 aagagtccga taatcggccc ggcccaagcc ataaatgtcg gccataacag tccgagttca
    43081 aaggaaagca cagtgccgga tacggcgcca atggcaaaaa gaatggcggt tccctttgcc
    43141 catcgtgtgg ccaattcacg aaagatttca ttgccggtgc gaatataacg ccattctgca
    43201 atgaccatga gtagtggcat ggccatgcct gccacggcga atatgatatg aaaaccaagg
    43261 gaaatcgcca tttggagacg cgcagccaga aggttttcaa tcatcttagg cgcccgctcc
    43321 tcagtatgat atagagtcgt ctcttcaatt tcgtcctggt taaaaagttt tgaaaaatgc
    43381 gatgtccgat aagtcctgaa gacggtcagg taagggattg cgctgaactc tcacttcgag
    43441 ggcaatgatg ggccctggcc tgtttaaggg catgggcata ccattccagt tcttcaaaaa
    43501 actcccgggc tttgtgatgg aatttttgat cagcaggcgt gccatcttcc tgaaaggcgt
    43561 cctgaacatg ggggatgggg aacagagagg gaatactcga cattcccagt tcagccaaca
    43621 tggccctgag ggtcattgcc gcacgaacgc cgccgaaccc acctgctgaa taacacacga
    43681 tggcggaagg ttttcgaaag tattcctcaa gaaaatggtc taatacgttc gagagggcag
    43741 gaggaatggt atgattatat tcgcccgaca caataatata accgtcagcc ggaataatca
    43801 gatcagccac aagttggagg aggtgaggcg cctgacccgg ctgatactct ttgtacattt
    43861 tatccaacaa cgggagagga aagagaagag gatcgatgag cgacgccgta tgcccacgtg
    43921 tttggcaggt gttcagcata aacctggcgg ccttgatacc ctgccggtcg gttctcactg
    43981 atccataaat tatagccaaa ttcagtggca tggagaattt tctcttaatc caaccattta
    44041 tatcgaatga tttctggatg aacagattat cccaaagtag aattttcagt gttgacagtt
    44101 taaaccgtgt gttcgaccac agggtgtaga caaatcgcaa gtgagccttt aaaaaagatc
    44161 cattgagcga aggaaggaag tacccataat ctgtcaatcg tgtcatccga ttgccgggtt
    44221 ccggaaagac gtcatggcaa aagttggcct ggcctaaatc ccggttcaaa aggagaagcg
    44281 attgaaagtc ggaatgtgga gagggtcatg atatctaatt ggatcgatat ctcaatgcct
    44341 ttgtatccgg gaatggttca aagacccgga gacccaccat ttcagatgga accggtgacg
    44401 gacagaaaaa atggggataa ggttcctgtc tcaaaaatat caatgagttc ccatgccgga
    44461 acgcatattg gcgctccggt gaaatgtgaa aaatcttttc aagataatga acaaacagaa
    44521 ttttcagcaa caataggcct ggccaaagtg attgagatcc aagataggaa ttctgtaaaa
    44581 gcggaagaat taaaaaaaca ccggatctat tcgcatgatc ggattcttct gaaaacacgc
    44641 aattccgcct ctgactggta ctcggaacac ccccctgaag atacaatctt tcttgaccga
    44701 actgccgcgg catggcttgc tgaacggcgg gtgagcaccg tcggaattga tgcgctgttt
    44761 attggaggga aacaccaaaa tggaaaagag attgctcgca tgcttatcca agccgggatt
    44821 gtcattattg aaggactcga attttctcga gtgaaaccgg gtgagtatat gctcatctgt
    44881 ttacccttaa aaattccgaa tggagatggt gctccggcaa gggccattct ccaaccggtc
    44941 cggtagtggg aagggaaagt aataggtcgc tcatgccgat gaatagttat ccaatcctca
    45001 agaacgaaat gatcaggagg tttattgatg gttcctgtga cccatgctta agttcggtgc
    45061 ccccacctct tttgccgaac atggctccct ccgatgcctg aaatccatca gaaggaattc
    45121 tcagaattcg aacgtaaggc ttcctctcca cctccttcat ccgaaatcga agctggttat
    45181 ctctctccat tatttcaggg accgatcaat atccggtcca ttgcccttac aggactgttt
    45241 gtctatgcct cactcatggt gatgtatgtg gccaaggcca tcatgcttcc tgtattcgtg
    45301 gctttatttc tgaatctttt gtttgctcct cttgtccggg gggttgaaaa aatacatatt
    45361 cccgccccgt ttgcggctgg ggggattctt ctcgttttaa tcagcaccct gacgtttgga
    45421 attattcagc tgtcgactcc cgcttctctc tggctggacc gtgccccgga tgccttacaa
    45481 caggtagagc ataaaatccg gtccatcaaa agttcagtcc tggaaatggg ggaggcgacc
    45541 aaagcactgg aacatatggc cagtctttcg gaatcccgga aagcccagaa tattcaagtg
    45601 aaaaccgaaa gtttgggtgg tcaattgatc tattggacca cggaatttat tctggggctg
    45661 gtttcaacca tgattctact gtattttttc cttgcgtccg gtgatttatt tctggaaaaa
    45721 ctcgtgaaag ttcttccacg attttcggat aaacgacgtg cagtggaaat tgtccgagga
    45781 atagagggaa atatttccag ttaccttgtc acggtgacca gtgtaaatgt tggtcttgcg
    45841 ctggcgacaa gtctcgccat gtatctgctc ggcatgccta acccgttttt gtggggagcc
    45901 atggcgggta ttctgaattt catcccttat gttggaagca ttattggttt aggagcggtg
    45961 acgttggcag cgatcctcac atttgaacat ctgaatttcg tggctgtggt gtccgggacc
    46021 tatttatttc tgacggctat tgaaggaagt gttatcacac cccatttatt aggacgaaaa
    46081 ctgactttaa acccggtgat aattttggtt agtgttttgt tctggggctg gttatgggga
    46141 gtcgtcgggg cgctgcttgc ggtaccattt gtcgcatccc ttaaaattat ttgcgatcaa
    46201 attgagccgc tttattccat cggagaattt ttaagtcgtt aagtttacaa tagttagggc
    46261 aaactgctgc ctactcgaat tatggcggga tgatgaattt cattgatctc gcgacagggt
    46321 aaggaactca tgccggcttg ggtattaaat tggttgaata aactccgttc gagtctctgg
    46381 ctcataccca cttgtatgac ccttgccgct attctcctct cgttggtcgt accgtatttt
    46441 gacaaatatt tttctcccgg gaatattgat tttctccaat ggctaagcaa catgggtcct
    46501 gaaggagcga tgactctcct ttcgaccatt gccgggtcca tgatcactat agcgggcgtc
    46561 gtgttttcca ttacgattgt ggccctcacc ctggcttcgg ggcaattcgg cccacgtctg
    46621 ttaggaaatt ttttgcggga tcgagggaat caagtagttc tgggaacgtt tgtggcgact
    46681 ttcctgtatt gtctgctcgt cttgcgaacc atccatgtgg atcctgatgg ctctacgcca
    46741 tattggggaa cgttggcggg ccttattttg gctgtgtgca gcctaggggt attgatttat
    46801 ttcatgcatc atattgctgt ttccatacaa gcccccaatt tgatcgctgc ggtatatggg
    46861 gaattggaag aagacctggc caggcttttt ccagagcgtt tgggactggc cgatgagaat
    46921 gaggcgaaca aaacccgtat tccttctatc ctgtccagga tagaaaaaga gggaatggaa
    46981 attaaggccg acattggggg ttaccttcag gcaattgaaa atgatgccct tatggaaatc
    47041 gctcgtgaac agaatttagt gatatttctt cattatcggc cggggcactt tatcaccaag
    47101 ggagcatcat tagccaaagt actggccctt gggccgatgg cagatgtcgt aaccccacag
    47161 attaacaagc aaatgatctg tggatccaac cgaacccagg aacaggatat tgagttttcc
    47221 gtacttcaac ttgttgaagt ggccgtgcgg gcactttcac ctggaataaa cgacccgttc
    47281 acttgcgtcc attgtattga tcatcttagc gcgattttat gccagcttgg gcaaagaaag
    47341 tttccatctc ctttccggta cgactctgag ggagttcttc gtgtgattgc tccgtctgtc
    47401 acctttgaag gcgtcatgaa tgcggctttt aatcaaatcc gacaaaacgg aaaaggcaat
    47461 gtggcggtga ccatctgctt gttggacggt ttgggacgaa taggtgaaac cgcggaacga
    47521 aatgaggatc gtgtggcaat ccttcgtcat gtcgaaatgg tccaaagagg gagccgggaa
    47581 tccatccagg aaaaaaatga cttagaggct gtggaggatc ggtgccgcca ggtcatggct
    47641 tctttaagta tgggagaaag ccggagttct tttttgatgg acaaattgtt gcatatcctt
    47701 ccctaactgt gtctttcact cctgctcctg ggaccattga tttggcccat gttagccctc
    47761 cggttcataa ttcgtcgctt aagactttcg gatttctacg catggtttcc cttgaatgat
    47821 tgaccgggaa cttccccgcc tttaccccac agacctttcc attacttttc atgcccgttc
    47881 cgaggcagtt ttttgacttt tcaaggttgg caataaaaat tttgtacatt gtcctggaaa
    47941 cttcagaaag tggttaatga atgatacagg cccctccact taatctttat cgaactcagg
    48001 ggactagatc cattttttgg taaacatgaa cgtaaaagga agactatgtt gaaaacgttg
    48061 ttggaaatgc gaggctatca attgcgagcc attgataaag accttgggag cgtggacgat
    48121 ttttattttg atgaccggtt gtggagaacc cgttatctcg tggcagatac aggaaactgg
    48181 cttcccggaa gaaaggtact tatcggacgg gaagcccttg aacggtcaga atgggaagac
    48241 aaggtcattc ccgtgaccct gacttccata gaaatcgaag atagcccggg gattgaaacc
    48301 gatctgcctt tttcgcttca aaaagaaaag gagttacgtc tgttttttaa atggagagaa
    48361 tattgggacg aggatggttt catccagcct gccggattca cccccatgag tgtcccaggc
    48421 ggggaagcta tggaacagtc gctcactgat ggaatgaggg cgcccggaaa attggatgga
    48481 aatccccatt tgcggtccgc aaatgaggtg cagggatatt acatcggtgc ccaggatgga
    48541 gagatcggtc atgtggacga ttttattgtc gaggatacct cgtgggaaat ccgatattta
    48601 gtgattgata ctcaaaattg gttgcctggg aaaaaggttc tcatatctcc aaaatgggtt
    48661 aattctatcg actggaataa acaaaccgtt caggtaggca tgaaccggga cctgattgaa
    48721 caaagtcctg aatatgaccc aagcaaaccc ataaatagag aatatgaagt ccgtatgtat
    48781 gacttttatg gtaaacccca cgattgggtt gaaaaatagt cgacaattaa aaaatctgtt
    48841 ttaaaggcgc catggtccgg tcatctacca tcatcgcctt acaatttatt gaatgccttt
    48901 tgacaacaaa ttattccgaa agaggaacga ctgacgttct atgtgaggga atcttttcta
    48961 tcgtaaatcg ggttttgagc acttactaaa ggagggagtc aatgttgaat acattaacaa
    49021 ataatcctgt tcccaccgta cttatcggat tgggattgta ctggttattc aaagatgggg
    49081 ctgaaccctc acacgagaca acaagacgcc atcggagtga caacaatcct tggcggaacg
    49141 gtgaaaaaga actccaaccc tcaatggagg agcaggtgac ggaaaaattc cgaacagcaa
    49201 aggatgccgt tcaaggtcaa gtgtcggagt ggaaggatga ggcgagtcac caggctcatg
    49261 aattgaaaga acaagccagt caacaggcta aggaatggaa agagggggcc gggcaaaaaa
    49321 tagaagaggt gaagggatat ttgcaagaaa aaaaggctgt ggtaggagga gaattcaatc
    49381 acctcctgga tagtaatcct ctggcagtgg gagcagtgat ggtggcacta ggcacggccg
    49441 tagcagccgc cataccggcg agtcggaaag aagaccaatg gatgggagat tcgcgagaca
    49501 gggtgatgaa ggcggttaaa tccagcgtgg aatcgacggt ggaggatgta aagcagaaga
    49561 ccggtcaagt tgcggaaaag gttttttcgg aagatacgaa gaattttctt taattgaaaa
    49621 aatgctgtgc aatcggacga aaggaaactt atgacttctt atgctaaaag aatttcattg
    49681 gttctgtgct tttctggatc actattcggt gccctggggt gtgaacaaga aggtcctgcg
    49741 gaacgagcag gcgaaagagt agatgaatca atggagcaag cgggagaaaa gatggaacag
    49801 gccggagaaa atatacagga ttcggccaat tagaaatctc cttcgaacag atggggaact
    49861 aatgtctcca ttttttctca tttgagtgaa gttttgtcca ttgtccgaaa cgtcttgaaa
    49921 ttgccacact gaattatgaa atcggtcatt gtccccggta gagacagata taaacgaaat
    49981 tcaggttctt tttttctatt accctgacgg aaaggagaaa agatgaccac tgccaatgca
    50041 aaaaaatgtg ctcacccttc ctgttcatgc gaggcccctc cagggaagga gttttgttcc
    50101 gaaaattgct cctcttctca accttccaaa ggcggaaaat gtcagtgcgg acattccggg
    50161 tgtgggcacg gaggataacg gcacgaatgc tcaaagaacg agcgttagtc gttgtttttt
    50221 ggatacagcg gaaatctaca atgaaggagg cgaacatgtt tactcccaac cacaagacgc
    50281 agtggtttct ggatgtaaaa attattatgg catttgctgt cctaatgggg gttccgggtt
    50341 tcgctgttgg tagcgatctc acaggtcatg ctcctgacca taatcgctcc acgacaactc
    50401 aaatacagga agaccgtcaa ttagatggag gacatcgaat tattcaagga gtggtggggg
    50461 aagtgaacaa aaatacggta cgggttgatg cgggtgaggc tggtgagatg tcccctcggt
    50521 atctgaattt aaataattcc gaggggaaag aaaattttga agttggagac agggttaaga
    50581 ttgaagtcaa tgctcaaaat aaagtcgtaa aatttcatcc actcaacagg accgaaggaa
    50641 agcagtagtt atcaccagtc tacgagtagc acattaatgt ttccttagtg gcggataatt
    50701 tattccaccc cttcggaaaa ggaataaagg agaaatcata tgttaagttg ggccattaca
    50761 tttttgatca ttgctcttat tgcaggagtg gtcgggttaa cgggtgttgc gggtacggcg
    50821 accaatattg cctgggtatt gtttgttgtg ttcttaatta tttttgcttt aagttttatt
    50881 acgggcaaaa gaccaccggc atagcaccgt attttgtacg ggaaggaaac ccccagttgc
    50941 tcaacggcgg agggtgaacc atggttgggc tttacgagac tgtggttatt gagtaaaaaa
    51001 ttcccgcaat ggaatttcaa taaagaccct gaagagggac gagggatgta tcatgattat
    51061 gatcatcacc cctgacgaga catttcgaaa ggcaatggtt ctccatttcc aagaaaagga
    51121 atatgagctt tcgatacccg agcgtagaca ggatgtccct tcgttggtgg aaaaacatgc
    51181 cccggacgtc attgtcctgg atatgtctat cgccgatccc aatgggccaa cggtcctaaa
    51241 agatctgcgt ggcaccggtt ataccgggaa aattgtcgtg atcagtggac cctccaatcg
    51301 gcccctcatg gtggatatcc atcaacagaa ggttgatcag gtcatgagga aatctccaac
    51361 ggatcctatt ccttccttga tggatcaact ggaaagtatt atccgggtca tgttccgtga
    51421 ggaaatttcc aaaaaagcct atgaccatta caataagcga ttaggtcaac atggaaaaga
    51481 ctgggaggac tggtttaagg caaaggagga aatattgaag aatccaacag gcgggagagc
    51541 accatcgaat ggtccccaac aaccataaac aaataatgtt gaagggtggt agaagggaca
    51601 tcagcgaatt ttatttctga aatggacgac ggggaaggga aaggattcag tgatatgacc
    51661 aggccaattt ggaaagggca catcaccttt ggtcttgtta ccattcctgt tgtcgttcat
    51721 tcagcggaaa aacaatttga cttgcatttt cggttgttgg atagccggaa taatgccaga
    51781 gtccggtatg aacgggtgaa cgaggaaacg ggtgaagagg ttccatggca ggacattgtg
    51841 aaagggtttg aatatgaaga gggtcggtat gttcttttaa atgatgccga ttttgaaaaa
    51901 gttgccattg aagtgaataa atcagttgaa atcgaaggtt ttgtcaacgg aaaggatatc
    51961 ggctacgagt attttgataa gccctattat cttgttcccg ataaaaaggg tgaaaaagta
    52021 tatgtgctgt tacgggaagt gctccgtcgg agcggaaaaa tcggtattgc caaagtcgtt
    52081 atccgaacac gccagtattt agccgctctg attcctcaag gacatgttct ggtgctggag
    52141 cttctgcgat ttcatcaaga aatccgcgat gtaaaagaat tggatgtgcc ggaagggaat
    52201 gtgaaatcct acaaaatttc agaaaaagaa ttgactttgg cccaacagtt agtggaatcc
    52261 atgagcacca agtgggaacc ccagcgctac catgatgatt accgtgatgc cttgttgaaa
    52321 tggatcgaaa agaaatcgag aagttctcgg tcacgaacat ccgtcccccc tgtcgcaaaa
    52381 gaagagaaaa caggttcagg aaaagtggtt gatatcatgt cattattaaa gaaaagcgtt
    52441 caaaaggccc aaccctccgt ccgagccaaa aaacgcaaat ccgcctcccc tcgaaaatcc
    52501 cgtaaggcca gttaacatac caccatgaat tgccggcctg actgagtaga agggtctttg
    52561 tttcggtttt ttaagaacta agacactcaa agctgctccc aggaagtcac aaacatggga
    52621 ttaaaggaat atcaaggtaa acgtcacttt gagaaaaccc cggaaccccg tgggacggcc
    52681 ttccggaaaa agagctcttc gtttgttgtc cataaacatg cggcaagccg gttgcattac
    52741 gatttcaggc ttgagctgga cggggtgtta aaaagttggg ccgttccgaa aggtcccagt
    52801 ttattccctt ccctcaagcg tctggccgtt catgttgagg atcaccccgt cgaatatgga
    52861 tcattcgaag gaaccatccc caaagaagaa tatggcggag ggacggtcat ggtatgggat
    52921 cgcgggcaat gggtacctca aggagatccc cagtattcct acgaccgggg acgaatgaaa
    52981 tttctgttaa aaggccaaaa gctgaaaggg ggatggagcc tggttcggat gggaggggca
    53041 aaagccaaag aagaaaaaaa ctggttgctg attaaagaaa aggattcgga agccctccga
    53101 gggaaacatt ccgatattat tgacatggcc cccgggagtg ttgccaccgg acgatcattg
    53161 gaagagattg ccgaggtgaa tcaagaaaaa agaaggaaga acccggcttc ccaacgggag
    53221 ccgaaaaaca accgttccac caaaagccgt tcctcccgta caccttcacg aaagaatatc
    53281 atttccggaa cttcccgtgt ccctgatccg tcagaccttc cttccgccgt gtcctcttcc
    53341 caacccgcaa cctttcatcc tcagttggcg actttgttca aaacttcacc tgaaggggag
    53401 gattggctgc acgagatcaa gttcgatggg tatcgtattg tcagcatggt taaaaatgga
    53461 agaattcgcc tgttgaccag aaacggtaag gattggacaa aaaaatttcc cgacattgcc
    53521 ggagcgctga aggcattgcc ggttcaacag ggtatccttg acggggaggt cgtcgtactc
    53581 cgatcgaacg gcacctccga ttttcaggcc ttgcaaaatg tcttgaaggg taaagcctcc
    53641 gaccctctgg tctactttgt gttcgatcta ccctactgcg aagggtataa cctgtgtcgg
    53701 accccgctca tcaagcgcaa agaactgtta tgggaattca tcaaagcttt gccggagaaa
    53761 gcggccgctc acgtccgtta cagcgatcat attcagggtc agggagaacg aacattcgaa
    53821 caggcctgtc gacgggcctt ggaagggatc atcagcaaaa atgccatgag cccctatcgg
    53881 caagcccgtt caaagcattg ggttaaagtc aagtgtcatc accggcagga atttgtcatc
    53941 ggcgggtata ccaacccttc aggatccaga aaatttttcg gagcactgct attgggctat
    54001 tatgataagg agggacactt acaatatgct ggccgggtgg gcaccggttt tacccaccaa
    54061 cgcctttcac gtatttatgc gcttctggcc aagcgagctc agtcccatcc tccatttgcc
    54121 cggctgcctt cggacagaaa cattcgggag gtgcattggg tacatcccga gcttgttgcc
    54181 gaggtagaat tttttgaatg gacacgcgac ggcatattgc gccatccttc gtttgtcggt
    54241 ctccgtgagg ataaaccggc aaaagaaatc atcaaagaga ctccacaccc tgtgaacaat
    54301 attctttccg aaccactcaa accttccaac cggcgatcca aggcctcgcc cgatatctct
    54361 acggaaaagc cggcgctggc gcttacgcat cctgaaagga tcctgtatcc tcagcagggc
    54421 atcacgaaac ggaatctggc ggaattttat caacagatcg ccaagtgggt gttgcctcat
    54481 gtggccggcc ggccgctgac cctggtgcgg tgtcctgagg gttctcaaaa aaaatgtttc
    54541 tatcaaaaac atgcctcggg atctcttccg gattccgtgc accgcattct gatccgggaa
    54601 aaagaaacga aaacaaagaa cacttatctg gtaattgacg atgtgaaagg gctaatcgct
    54661 ttggtgcaaa tgggcgtatt ggaaattcat ccgtggggat gccggaagga tcgaacggat
    54721 cgacctgatc gcctggtcct cgacctggac cccggtccag gggtgaaatg ggaacagctg
    54781 gttgaaggag cccatatgtt aaaaaaacgg ctctcggggg atggtataga aagttttgtg
    54841 aaaacctcgg gaggaaaggg attacatgtc gtggccccct tgacgcgccg tgccacgtgg
    54901 gaccagttga agcagtatac tcggagactg gcgatggaaa ccgcccggac gcatccctcg
    54961 ggatttgtgg ccacgatgag caaagccaaa cgaaaaggga aaatatttat tgattatctt
    55021 cgtaacagtt tcggtgccac ttctattgcc acctatggca ctcgagctct ccccggagcg
    55081 cctgtgtcaa cccctgtgag ttgggatgaa ctcttttcta tctccggttc ccaggtcttt
    55141 actctcacca ctctccctca acgccttcaa aatctaagcg ccgatccctg gaagggattt
    55201 tttgatcttc ggcagacact ctctcgatcc cttatctaat ttccaaaaac gaattcatgc
    55261 gcttgcgtga aggggacctc tccgatgccg ttcatttttt ctttcgggca cgcgtctcag
    55321 cggccttcct ggcagaggcg gatttggcgg atgccgatcg tgaggccgag gcttttccgc
    55381 ctttttcccc gccttttttt gccggtgctg tattcatcac ttttcctcga ccggatccgc
    55441 ctggtttctt ccctccaccg gtcattttgt tcacggtagc ccatgcacgg cgttccgctt
    55501 cgtcctccgg tacgcctcgc ttttcatatc cttcttcaat tttttctgcc tgacgttttt
    55561 gctttccggt gtatttggat ttgtctcctc gtggcataag gacctccttg ttgctggcag
    55621 agccgagaag aatgtgagaa aaatgatttg gagtgaacgg tcaagataga atggtagggg
    55681 aatacgcaca actatcaaaa accctcattg accaaaacgt gtttaggaac gtgtggaatg
    55741 agtgtgggtt tcagctgtcc gacccaatgt atctgccgaa taggcttggc gttggcccat
    55801 cggttcgggt tcaccctccg tcgtgtcagc ccgcgtctgt cgttccattt ccgaattaat
    55861 ttcggctccg accatgacga tgaatgcggt taaccagaac cacatcagca gaatgactgc
    55921 cgcgccgagt gacccgtacg tctggttgta actactgtaa tcattcgcat agatagcaaa
    55981 ggccgacgat cccactatcc acatcaatgt ggcgattccg gctccccatg acacccactg
    56041 ccatctcggg taattccggc agggcgcata ccggtacagg atggccaatc cccccatgac
    56101 gatcatggcc aggagcggcc aacgaatgag agagagggcc gtttgcattt tcgggccgaa
    56161 tcccagcgcg tccaatactg ccggcagcag ggtaatgaga aagagagtca gaatgacaaa
    56221 aattagacct ccaaccgtta atcctaatgc cagcgcggta actttgatga aactccgttc
    56281 ttcttcttca ttgtaggcaa tgttcaagga cgtgatcatt gcggccatgc ctttggacgc
    56341 actccaaatt gcaaagagca accccccgat cgctccaaaa cttaaggcag attccccgct
    56401 ggttgaaata gttgtcagct gctcttggat cagagtttgg gtgtcatgcg ggagaaagga
    56461 actaagagaa gtaatctgtt gctggatatc agaggcttcg gtaaacagtc catacagcga
    56521 gacaatggcg gccagcgccg gaaagagcgc gaggaccgca taaaacgcca ctcctgcggc
    56581 gacaatcgaa acgttatcct gtgagatctg atccttcagg cgcaggagga tgtcccacca
    56641 tcccgcattg gggatttgac tgggctgggt agcattccgg cctctcgtgc cggacttctg
    56701 ttcagctgtg ttgtcttggg acataatcta gcctcgtgtt gtaaagatgt ggtccgacag
    56761 gaaaaatttt aaaactgctt atttggcaca ccggcttatg caactcttta tgcggtgctc
    56821 ctccttcaat tcatgccgac ctgttctttg atggtctctg ccgcaacccg ctgcgcctca
    56881 gcagcggctt ctttggcctt cggcatgatc tccttggcag ttgctttcgc ttcctccagc
    56941 aaccggtcgc gtgtttcccc catccattga tcttcctttt cagaccgagg cagggaaagg
    57001 ccgatagctg tccccatggc taaagttacg gctccgatgg tcagagggtt ctcctgcata
    57061 tagcggaaca attcatgctt catacgttcg cccccctcgc gtgtgcgctc tcggatttca
    57121 tctgaccggt gcgatgcttt ttctttccaa tcttccaatg attcttgtgc ctggtctgtc
    57181 cattctgaaa tctgttcccg tgcctgttgc cccgaggtct gtgctcttgc cttaatggcg
    57241 tccagggggg atcttccccg ccgggatcgt tgacggggaa tccgcccggc ttcgctttcg
    57301 ggagcataat ccggccactc ttcataggca tcaagggagt agtcatcgcc atatcggtcc
    57361 cttccaaatc tgcctctggc tgatgaggaa gtgccggcct gcttcatcat ccataggaga
    57421 cccacgccaa taagggcggc aggtaggggg ttgttcagga cccgttccac caggatggat
    57481 tgccattggt tcaccttcac acgggcattt tcttttaccg tttcgacttt atccatggct
    57541 gcggttttga cctgttcctt gatagtttcc ggggacagac gttcttgaag tgtatgaacc
    57601 gtcccatcca attccgccct cgttactcga atttgttcgc gaagccgggc aggatcttca
    57661 atctttttcg attccagtgc atttaattga gtaggttggt cgtccactgt ttggtctcct
    57721 ttaaactgtc tatggttttt tgcggagcag gatccatggt tttcagatcg ctgattcctt
    57781 tgtgaatcaa tgcgtagccg atccccacaa cggttagtcc aacaatcagc gccgaagccc
    57841 aggcgggtat gattaatgcc agggcgagag tggccccacc taaaagaacc agtaatccgg
    57901 cataggccag cgctcctcct actgcaataa atcccacatt ttttcctgcc tgtttggcct
    57961 tttgggacat ttcaagcttc aagagctgca gttcctgctg aatgaggatt tttgtttcgt
    58021 tcaccaggtc acggaagaga tcactaagtg gtcgttcttc ctgatataaa ttcataacat
    58081 cctccttcta tggtctcatt gatgtgaata agaggatttc ccctttacat ggttggcgcg
    58141 gcaggagtag ttgtcgccgt ggcttcttcc cttcggaacg attcctgccg tccatatgat
    58201 gaggattcgt ctatccttcg atctcgggaa ctctttagaa atcttccggc tacaagcccc
    58261 agcataaaag ctccccctaa aaacgcttcc ggatggcggc gggcaaacat ttcagtctcg
    58321 ttcagca
//
LOCUS       DAORAQ010000003        45896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_179_length_45896_cov_70.974717, whole genome shotgun sequence.
ACCESSION   DAORAQ010000003 DAORAQ010000000
VERSION     DAORAQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 45896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45896
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179_length_45896_cov_70.974717"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKM72_00615"
     CDS             <1..296
                     /locus_tag="PKM72_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59303.1"
                     /translation="VMDSGEGRLVAGHRFGSFTPSFDGVQLTLGGDESSGWGLRAFVT
                     QPVQRHTVRPDWTSPINYFSGAAISSRHVPWANGELYLNEFHYPSKGKEASFS"
     gene            561..2360
                     /locus_tag="PKM72_00620"
     CDS             561..2360
                     /locus_tag="PKM72_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNP59304.1"
                     /translation="MDHVTAGIREYFKDFNFKENFKGITSNVRGDVLAGITVAMVVLP
                     MALAFGVASGLGAIAGMWSAVAAGLIAGPLSGSAWSVGGPTGPLTIQILSMTQTNKLA
                     DGSPDLVFVFTTVALAGFLLIVMGLLKLGQFIKFTPYSVISGFMTGLGVLYMLLQLNP
                     FLGLPGVKSITSAITELPSSLAHASPVAMGVGVLTLLIVVYWPKISPVTWLPGPLIGL
                     WAGTVTTVVFGLDIPKIGDIPTGLPELYIPDLTLLEKAFVPAAALAGLCVFDSLLTCL
                     IVDNMTGTHHNSDRELVAQGSANLFSGLVGGLGGATNTMPCVVNIQSGARTRLSSVTM
                     GLVLLSFILGLGSLAASIPLSALAGILLKAGYDILDMRVLPVVRRLPTSDLMVFGLVV
                     LMTVFWNLLSAMAMGLAVAFFRFVKDQSDRYKADLSQRDEDVKQEEEDLIFSFARDYA
                     RKISQNGVNIGELSGRFEHIVRDRLIIVRPHGPLFFGAIDWLNETVEHLDGKDVLIIR
                     CKWLDELDLSGAYALGDLIEAANCRGVAVLTAGMSPRTRQVLADLNELSRLKDDYICT
                     HFNEALDIAMQIVEKKAVDAGPQAHKEPVLAFS"
     gene            2778..4703
                     /locus_tag="PKM72_00625"
     CDS             2778..4703
                     /locus_tag="PKM72_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59305.1"
                     /translation="MSLNKLFLNLPIAKKLLLVSGVPVLAVLILSLVTYRSVQTFSLD
                     EDRLNDVYHVQSASSEYMRLVVDLETGFRGFILTQQTPFLQPYLAARQRVMLLGNSLR
                     QMVEPIEDQRQLIESVQKMIGQLMNDKDRIIERVKAGHPEEAMNYVESEKGRTLMLAI
                     REEMARFDRREVMQLQKALGSSAADRAALMGVVVWGGSLALILLLVPLHLIARSITGP
                     LTTLVKAVENVSGGTIPSIPVFQRDDEIGELTKVMQTMGTQIRNHIRRIEQSEQELRT
                     LNHDLTSSEAKYRGIVDYAPIGIFTAKEVHLIFSNRQNWILAGRSSDEVLDPEHMWEA
                     IHPDDREGVRETFQDACHQGEPFERVFRFLHPDGTVRKVLCRAVPIQDEGGQTMVYQG
                     FNVDITALEHMRAKLNRSERLATLGQMAAGIAHEIRNPLVGIGSTTTLLMDDFPEGDS
                     RHDDLVTILKETRRLDRIVNQIVDFARPRDLLPVTFQLKDLVLESLQVLNEPIKQKNI
                     QVEFHPSANLDTIQADRDQLKQVLLNVIQNAVEAMSDGGSLCLSIEEESIERKPGLRL
                     KVKDNGTGISPGALPRIFEPFFTIGKNRGTGLGLPICRNIIEAHGGEIHAESQSGLGT
                     TMTWWLPCVCQPQLPTV"
     gene            4707..6095
                     /locus_tag="PKM72_00630"
     CDS             4707..6095
                     /locus_tag="PKM72_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP59306.1"
                     /translation="MRANIFVTDDDDVVRQAISRRLAKRHHQVRSFASGEALLEALDH
                     DIPDLILLDLKMAGLSGLETLKNIRAKSQQALVILLTAYGTVEEAVEAIKLGAYDFLI
                     KSVDFSSVEPVIDRALDYLSLRRRIDFETQDRPGRYALNGLISNSPSMKELVGQIQEM
                     AKNLKTTVLLFGETGTGKEFVARVLHHNGPRDKGPFVGVNCTAIPQELFESELFGYER
                     GAFTGANQRKLGLCEQAEGGTLFLDEIGDLNPSMQAKLLRVLQERSFKRLGGQEDIDV
                     DFRLIAATNRDLRKDVTQGRFREDLFFRLNVVSMTLPPLRNRTEDIIPLALATLIRQS
                     SELGKEVTAIEPEAQRLLERYPYPGNIRELENIMERATIFCRGKSLTEGDLPREVHEE
                     ARSSVNAVARGDQQVVRMEMILGEQTLAAIESDLIEEVMRLSDYNKSLAAKYLGITRF
                     ALDRRLKKIPES"
     gene            6699..9020
                     /gene="priA"
                     /locus_tag="PKM72_00635"
     CDS             6699..9020
                     /gene="priA"
                     /locus_tag="PKM72_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNP59307.1"
                     /translation="MLADVVLPGRRFQVFTYQVPPHLLSLLHVGSSVVVPLGSAVVSG
                     VVVTIFEAKSSSSLQSQFRHKPLRDILSLEGDVGSSPLEHNLIRLVEKISDYYLAPFS
                     ACLRLIVPPHSIQVIRRLILTDDGRQALLDRSLPSDVQLVLRKLERAPRGLLRSSLIR
                     TMNNVPAFLTRAKKKGWITEQTIMSSGSKGPSPVRGISAGRKPVPSIIRGLFDSPEED
                     ARELPESARRMESSSFHDNRIWKNLVSAVQSGGFQEVPVVGSESVRQDLLLKLIQTII
                     DQGRRVLILAPEVQEVEILGEQVRFRGRIQVEIYHGHLSTIVRAARWERIRQGDVQVV
                     VGTRSALFLPIPNLRLIWVNQEESSSFKDEHLPYFHARDVARMRGDCEQALVVYGSTR
                     PSLELYGRFPEQVGEALEGPSQQIPQVEMVDLRTLPYETILSPVLLSRMTQTLKEGEQ
                     LILLLNRKGFSGALVCRDCGKTPICPTCGVPMKLYQRPSRLVCTYCDGIGPTPEICPT
                     CHGRVFRFSGTGTQRLEDEVTRLFPDVSVARFDRENVKTPEVADAILRQFRQRKIGVL
                     IGTEFLVHQSNPPMAPVIGFPQADLGLHIPDFRSAERTFQMLSRAFTLARNGQEPGEV
                     ILQTRIPDHHVHTAIGHHRPRMFYDHELQLRNLLGYPPATHVILVVVTGEQKPRVQRV
                     VDFLHQRLKAFEARRVPCAEGKGIIGASMILGPMVSKKPGRIKKNRVLFLIKTDDLSE
                     AQRGLRAIQLEYEAEFHKDPVIVEFNVDPMDIQ"
     gene            complement(9034..9480)
                     /locus_tag="PKM72_00640"
     CDS             complement(9034..9480)
                     /locus_tag="PKM72_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59308.1"
                     /translation="MNDHLWHQLNAVLGSLTITVGLWLLVGALPVPIGIALAIGLAIL
                     LAWKCPSIGLIWATSTLLLGVESLAWPIMQMADLQKLGPEPPLEELERIFTAVLFGLF
                     SGVFWMTFAYGIFKRTRDKNSPPAPSNTVPKTKNQKKQSRRQSSRV"
     gene            complement(9590..10396)
                     /locus_tag="PKM72_00645"
     CDS             complement(9590..10396)
                     /locus_tag="PKM72_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNP59309.1"
                     /translation="MSAEPSSFHEPPPNPFGGKTLIVDAKRPGSFTLPSEALAQAQKD
                     DQIFIRPGVYEDRLVMTERTIHLVGAGRDLVTIFNRRSGPCYLQRVSGGNITGITFRY
                     VGSDQHSALNILDSVCTISHCRATEGLLSGVVIYGPDCRPTLLANEVSHNRESGIFSF
                     AGAQPYLRENACFGNHHFGIAARDEGTRPDMIKNICRGNMLSGILLFHLAKALILDNT
                     CAENAHWGIVLTPDCETTPTPEELHQSNHLENNPRGAYSLTTEPLNDIGR"
     gene            10876..12414
                     /locus_tag="PKM72_00650"
     CDS             10876..12414
                     /locus_tag="PKM72_00650"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNP59310.1"
                     /translation="MSFVKGLSGAQAQETDIFQHPNSEQAGIKKSLEQQIGAGRGDIN
                     TPDSSLYIINRDPFRSIRRGRNLFQRKFTISQGMGPRNGDGFGDISADASLGAGLADS
                     CAACHGRPRSSAGFGGDVFTRPDSRDAPHLFGLGLIEMLADEITQDLRKIRDQAIARA
                     KGGRGVEVSLKSKGISYGKIRVNPDGSVDPSHLEGVDPDLRVRPFFHHGGTISIREFV
                     VGALNGEMGMEAFDPDLAKAADGEEVVTPSGMVLNGKTDTIETPPAFSESDDSDRDGV
                     ANEVPVSLVDHLEFYLLNYFKPAIGRETKDSKRGQRLFQDIQCTSCHIQNLVIEKDRR
                     VADVETTYDRRRGIFNDLFAVATPLFQEVQGSGSPSVKKPKGQRFVVKNIYADFKRHD
                     LGSTYWERNFDGSLTKEFMTEPLWGVGTTAPYGHDGRSIDLWSVIMRHGGEAKDARDR
                     FARLSEEQQGQIIDFLQTLVLFPPDDTASNLNPGDSQAQNFPQYGHGNIALPALFNNP
                     SDVE"
     gene            complement(12759..13412)
                     /locus_tag="PKM72_00655"
     CDS             complement(12759..13412)
                     /locus_tag="PKM72_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59311.1"
                     /translation="MLTHDEKKASEAAFRGLPMNDSWTDSAKAVYEGLLQALGKTPLS
                     DLAPLEKDDTDSQDRSNLLPSNLSESSQLALSLGSGDIPSEFESLPIGSRKEAIESGI
                     LKDVTTIAQSVGLDFCVGMTNPLWDKYITPSPDYPEELIQTRLRDTLVAVRLRLAILK
                     TPTPLVDVPILLQFSPEPTPQLCLTFALFHKDPVDGDCLLLIHPGEVFATRQAFENN"
     gene            complement(13597..13968)
                     /locus_tag="PKM72_00660"
     CDS             complement(13597..13968)
                     /locus_tag="PKM72_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HNP59312.1"
                     /translation="MKHTVTRGEMESAIRNAIIKFEQEFMGRGPTDVRASILRDTILI
                     RLKGVLTPAERQLAKSEEGIGMVKQMRQNLISQGREQLCREMLEITGAETTALFTDID
                     TRIEERIIVLTLDRDLEATFR"
     gene            14487..17513
                     /locus_tag="PKM72_00665"
     CDS             14487..17513
                     /locus_tag="PKM72_00665"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNP59313.1"
                     /translation="MNKVEQDPLSVKSWKWLRDIVDEACQPIGPYWPMKSFAYYNPIR
                     DLEHLPFARAIEAANQLLGAKGFLPVGEYRQLFHQGRITLSAIDQALRRVGPPLPSRT
                     TVQVGDRSIHVQDVWRIHAIYGIEILPAVLLTWTLELEGLNTRFRSDLPENSRTSIIN
                     RTIRECEKCRHDPESAYLHNLWKSSLAACQLFDPVSGEGSFHASDLGRSSHLSGKEAY
                     SVTVDLPKDRTLSDWLDSETGSALVETINTHMIKWIAAFVDEGVAGWSMPSRDKGFFA
                     AWRELAEGDLSGKFLGIPDLRQKFRELSDVPEEMLCKHLQNLNIPKERWQEYLCRHLA
                     QLPGWAGFIRWRGDHPGYPAQQHYPIDPLQYLAVRLFYESGMVEGLCQREWGIKGTLP
                     GLLAYWNKQGTHEQALSLSSSHATVANDQAVCHQAWRLFHLAQFLELTPIEVHELSYS
                     DTSTLLEWLDLFPQSSHGPVWLEAYEDVYRESLLRNISGHQAVAPVSNQRPRAQGIFC
                     IDARSESFRRHLEAQGPYETFGYAGFFGVPMSHVAFDSHDHLALCPILLTPKAEVIEV
                     PRMGQHERVKSYLSGTRWHQFSHHLFHDLKHNPFASFMLIDVLGMFFSVGLVGKTLFR
                     TSFDAIKQRLHQWLGSTVVTHIPVEPSGDDEHKNPPVGELAQGFTTLEQAAFVEGGLR
                     VIGLTKNFGRFVMVCGHGSQSDNNPYYAALDCGACGGSHGDPNARAFSAMANNPEVRQ
                     ILNDHGLVIPEDTWFLPAKHNTTTDQVMLYDLVDVPAIHLEELGLLVRDLEQAGTHQA
                     LERCGRIPGAPTAVSPNEAFKHVRQRSMDWANSRPEWGLSGNAAFLIGRRALTKGLDL
                     EGRVFLHSYDPGSDLDGSLLEKIMTAPLIVGELISLTYYFSGVDPWAYGSGSKVIHNM
                     VGGVGVMLGSQSDLQKGLPLQSVNDGARHYHEPMRLLAIIEAPPDRIRSIIQKHTLLQ
                     HVFHNQWMNVIAFDPDSKGFSRYLSDSTWEPVTTTI"
     gene            17992..18483
                     /locus_tag="PKM72_00670"
     CDS             17992..18483
                     /locus_tag="PKM72_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNP59314.1"
                     /translation="MKSSYPKQFIANTLALFSVWLVLSGKYDNFHLVLGFMASCGVAW
                     LNTGFPHSPFHNFPWARVVLYSPWLFLRIVESSLHLTKLILNPSLPISPRLITYQSHL
                     KHKGAIVLLGNSVTLTPGTITVEANGNTLLVHAIDGTAAKDLTTGRMERKLAWVFQED
                     TNH"
     gene            18483..18749
                     /locus_tag="PKM72_00675"
     CDS             18483..18749
                     /locus_tag="PKM72_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HNP59315.1"
                     /translation="MRDFFLFLLITLSFLILVYLYRVIQGPTVFDRVLGLAGISTKAI
                     ILGVVMGTVYDRVEMFVDISTGYALLNLVGAFAIAKFLEQRGTP"
     gene            18750..19049
                     /gene="mnhG"
                     /locus_tag="PKM72_00680"
     CDS             18750..19049
                     /gene="mnhG"
                     /locus_tag="PKM72_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNP59316.1"
                     /translation="MVVLAIGLILVGVFFLVVAAIGTLRLPDVFSRSHAVSLTDSLGA
                     ICVLAGLALYQGFGINMLKILVVLVLLILLNPVIAHATIRAANRAGLKPQTKEDP"
     gene            19046..19579
                     /locus_tag="PKM72_00685"
     CDS             19046..19579
                     /locus_tag="PKM72_00685"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNP59317.1"
                     /translation="MIYSVNIALLLLLVLTAASATFLVKELMSSVFILGSYSFFLSLV
                     WAWLGAVDVAFVEAVIGAGLGTVLFLITLFDTAPKDSRLRRPPPPLAGVLGLPLLGLL
                     LLLAANDLPQFGDPTSPPNVHISPVYLQNSYADTLTPNVVTSVLMDYRAFDTLIETVV
                     IFTAGIACALLLREPGS"
     gene            19576..20025
                     /locus_tag="PKM72_00690"
     CDS             19576..20025
                     /locus_tag="PKM72_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNP59318.1"
                     /translation="MKQIHDSIIVQSMSRLLIPLAQLFAFYVLFFGQYGPGGGFVAGV
                     IWTTSMILAFLVFGLKSPQGRLVEKVLHGDGIGLIIFVGVGGLCLIGGGEFLNYANLE
                     IPGLDAPARRYMGILLAQIGVAVDVAVTGISIVLSLAYHDTEQEYDV"
     gene            20015..20395
                     /locus_tag="PKM72_00695"
     CDS             20015..20395
                     /locus_tag="PKM72_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HNP59319.1"
                     /translation="MMFELIGGWLQRPNYIAFVLLFLWGIYIMVTRYNLVKKLIGMYL
                     MQTSVIFFLVTTSAKRDATVPILLSTTEIIQPEVYANPLPHVLTLTAIVVGVATLGVS
                     LALADAIYKKYGSLDEEEILKRME"
     gene            20392..21861
                     /locus_tag="PKM72_00700"
     CDS             20392..21861
                     /locus_tag="PKM72_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNP59320.1"
                     /translation="MTRHLPVILFLLPLGLAICLPLIGVRRREWCRPLAIGVLAAMVP
                     VSFASLAGVLQNGTIHYDFGGWAAPVGIEWVADGLASIMTVALSLVGLVCITFLSSVL
                     FPYLGSRIVPFYTLVLLLIASLIGMVFAGDLFNLFVFLEVSALCAYALVGLAGGKALV
                     AGFRYLILGTFGASLYLLGVGYLYAETGTLNMADLAQRVPPLVGSKAIAVGVLFMFIG
                     LGIKMALVPLHGWLPDAYTYAPDRVSPMLASLVTKVALYGWIRIMFWVLGAHAVVYRI
                     PVLFLVGVLGALAALVGAFLALSQTEIKRMFAYGGLSHIGLILVGISLGNQTGFVGGV
                     FYLLNDAVMQAGLFFWAGAIIHLYGVRTIEDLGRLQGGAGWMSVALVVIALSMIGIPP
                     TGGFFGKWYIILGAVEAQNYLAIVAVLIATLLTLAYFAKLFERLFRDRSAQQPLPIRE
                     IPLAVRIGLGATSVGVIVLGVMSDHIVSFLLKNALPPGL"
     gene            21891..24068
                     /locus_tag="PKM72_00705"
     CDS             21891..24068
                     /locus_tag="PKM72_00705"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNP59321.1"
                     /translation="MAFLILIPLLPLMAVLILVLGKPWLGEQGHKIGIPAMALSFACS
                     IIAFAQVATDGAFSIPLYRLIHSGDLVVDLGLYVDQLTVLLLLLVTGVSSVVHVYASR
                     YMIGDPRYNRFFAVIALFTFSMIMLVMSNNLLMMFVFWEIMGICSYLLISHWGERQPS
                     AQAATKAFLVNSVADVGFGFGVILTYSTFGTLDIQTILAEAPSIQGNTINLFGWIGLD
                     FPIHIITLITLFLFLGAMGKSAQMPFHVWLPFAMEAPTPVSALIHAATMVNAGPFLLV
                     RMSPLVILSPDAMTVIAVIGGLTAIFATLVSLTQVDIKRMLAFSTIGQIGFMIMTCGV
                     GAFVLSIFHMLAHGCLKGFLFLSTGNALRVVGSHGHEPTSFSPHVSSNHSLWPIVSGA
                     LVLACFPPFLIFFGPYEQLWMIQPGSSGQWTFWILGLLTVFFSAMYVMRGALLLFNQP
                     LSTAREDGMGSQAMKPRFFSTIHLLGILAIIGIVGSLLLILWSWFAEFLSPVVGHRMP
                     YIPEKSSSGPGLQGILAFAVAVCGIVVGFFSMTKFSGSWFQQSEMGKRWYVHFLNKLY
                     FDEMYEAFVVAPTLWFARWLWRVVDRKVFDALIMGIANGSVLAAKWMWRVVDLRGIDG
                     LVVGLGRNSVGIAGWLWRVVDLRGVDRVVVGIGQRSLGIADWLWKSIEIKVLDKNVNR
                     AGNQAETTGNLMRELEPRTLQHHLLVMIFWLILGIGLLFWLVV"
     gene            24205..25713
                     /locus_tag="PKM72_00710"
     CDS             24205..25713
                     /locus_tag="PKM72_00710"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNP59322.1"
                     /translation="MNTIFIEHLLSVMIAVPFVGIAIIACIQDQEWIRRVALGCTMGD
                     GALSLVLLKEFDFSIQGMQFVERMAWMPTFNIQYAVGVDGISLLLVLLTTLLCPFCVL
                     CSWQSITTRVRSFMAMILLVEGAMIVVFTALDLFLFFMLWEVTMIPMYFMIILWGGPQ
                     RIAAGIKFVLYSLSGSLILLLAILGLYVEGGRTFDILALAEKTYSPDAQFWIFLAFFL
                     AFAIKLPMLPFHTWLPDAHSEAPTAGSVLLAGVLLKMGGYGFLRFCLPMFPEASVTFA
                     PFILWLSVAAIVYGGYMALAQSDLKKLVAYSSISHMGFVTLGIFVFNNHGIQGAILQM
                     VNHGITTGALFLAVGQLYDRTHSRSIQDYGGLQKSMPRFVALFCLFSVASFGLPGTCN
                     FIGEFLVLVGTSFESYVMVLLSMGGIVLAAAYMLWMLQRVVLGQPKNSTIAQLPDLSM
                     RELATVIPLAVLILGIGLYPSPLMEMMDSSVTHLAQHMAQYQVGMIHEVALR"
     gene            26183..27262
                     /locus_tag="PKM72_00715"
     CDS             26183..27262
                     /locus_tag="PKM72_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNP59323.1"
                     /translation="MKLINVLLVAVACTGVVGCVSQQSHERALQEYEKSQESTAVELE
                     RLQQAVEEKQTEVMQLQETKMALEHTVEVQDQIKEEQLAAVQQEVEALYTQIRKLGNT
                     EGVGSLPDHLFGGEPVDFQDLRAALSRIRSGLDGQIGQVSHLREENSLLEKELSALEA
                     RLKEVERLKHELELVKKAREAQEAQLAKVKYEVLTVGEEIDRITKALEDKFGKSLVVT
                     QHHDRLVLTMLGQVLFESGEADLTPLGLTIMKQVGQVLATLPGKNIQVEGHTDNHRIY
                     GQLQKQYPTNWELSTARATTVLRYLIEQTGMDPNQFSATGYADRRPVMTNETEEGRAQ
                     NRRVEIVLYPERVVQDIETVATLAR"
     gene            complement(27412..29004)
                     /gene="nhaB"
                     /locus_tag="PKM72_00720"
     CDS             complement(27412..29004)
                     /gene="nhaB"
                     /locus_tag="PKM72_00720"
                     /inference="COORDINATES: protein motif:HMM:NF007093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HNP59324.1"
                     /translation="MKTTVPQALIQNFLNHTPTWYKLTILGFLILNPILLMSIGSFYT
                     GWVLILEFIFTLALALKSYPLQPGGLLALEAVLLGMTTPATVYHEALNNFQVILLLIF
                     MVAGIYFMKDLLLFLFTKILLGVRSKVLLGLLFSIMGAFLSAFLDALTVTAVIIAVAL
                     GFYNIYHRVASGKSSQTSHDSTSDHEIDSLHRENLDNFRGFLRNLLMHGAVGTALGGV
                     CTLVGEPQNLLIADKAGWEFLEFFLRMAPVTLPVLATGLFTCIMVEKLRWFDYGFQLP
                     DPVRLILVEFETEQQKRRDSADTAKLIIQAIAGIWLIFALAFHLAEVGIIGLTVIVFI
                     TAFNGIIDEHQLGEAFKEALPFTALLVVFFAIVAVIQDQNLFSGIIGYGLSLEGSHQI
                     GMFYLANGILSAISDNVFVATVYVQEVLRAYQAGTITRDQFDLLAVAINTGTNIPSVA
                     TPNGQAAFLFLLTSSLAPVIRLSYGKMVWMALPYTLTMASVGLFAVYMVLDPATAYLY
                     DTGLLQHHSPSSSGNPTVPAAH"
     gene            complement(29202..30911)
                     /locus_tag="PKM72_00725"
     CDS             complement(29202..30911)
                     /locus_tag="PKM72_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNP59325.1"
                     /translation="MAATFPILSWVHGLHFNNIRGDLYGGLVAAVVALPLALAFGVTS
                     GLGAIAGLYGAIFVGFFAALFGGTPAQASGPTGPMTVVMAGIILNFADEPAVAFTAVI
                     LGGGFQVLFGLMGFGRYITLVPYPVISGFMSGIGGIILILQVAPFLGLEAKSKVVANL
                     MAMPEYLASFSLPAMLVGSLTLAIVYLTPASIGKRLPPVLLALIVGTTVGEMFFPTLP
                     VIGDIPSGFPTLIWPTMHTSTLLLMVEEALVLALLGTIDSLLTSLVCDNMTRTQHDSN
                     RELIGQGIGNMAAGFFGGLPGAGATMRSVANIRTGGRTPISGMFMAVVLLGTLLGLGP
                     LAEKIPLAVLAGLLIKVGFDIIDWRFLKHMVQAPRTEVLVMIVVLLVTVLVDLITAVG
                     IGVVLASVMFVKRMADLELENLSVITGASNETPLLKEEQRILDRNCERIIMIHVNGPM
                     SFGSAKDMVRRLESVKGFNSFSSVVLDLTSVPSIDGTAALAIEDMLLMVKTHQQHVFF
                     VGMRPGVARVLKGLGVLDQVPPEHRYPLRLDALRHAAHIGGSTHPFDTPPTEVLSKNP
                     EPS"
     gene            complement(31035..32756)
                     /locus_tag="PKM72_00730"
     CDS             complement(31035..32756)
                     /locus_tag="PKM72_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNP59326.1"
                     /translation="MPSPLTLQSSGERPQNNRGQILSLALMVASVVFVVDFFLPTGIA
                     EGVPYVAVVLIGLRSPEKNDSLRLAGLCTVLTIVGYGLSPAGTEPWIGMINRSIALFA
                     IWSTALLAMQVREADAATRDQSSMLTGILSNMPAVAFRIDQEGVMHDSFGRGLTRFGL
                     KELTAIGRIPLEPPDRIKSEIKEGTLANSVFYESNGILQGKPWWFLNCLCKLEGDQPG
                     LIGFGIDITDRKRAERRLALHDAIADILTTASSISETYPQILKAICHTLDWRVGILWR
                     MDYQRQALGAAVIWPQDSPQAQAMSSSNSGWALTPAVGLAGQVWDNRSPLWIKDLADY
                     PKDPRNQAALAVGLHAGFAIPICLAGNTRAVLEFFSPFPEEADDMLLQRLANIGFQIG
                     QGIEREHKERRLATHHGLTNVLAESAGMTQAASLILEAIGENLGWDLGAIWILDSSQQ
                     VLRCLNVWHSPHLNLDAFRQASHAITLARGVGLPGRVWKGGEPSWVTDVVSDPNFPRA
                     DAAAHEGLHSGFAFPIKSGNEILGVIEFFHRDIQKPDKELLAMFASIGLQIGQFIQRT
                     TKPGLNG"
     gene            33052..33423
                     /locus_tag="PKM72_00735"
     CDS             33052..33423
                     /locus_tag="PKM72_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59327.1"
                     /translation="MSSTSPQVHIYHVAGLDDRDRGFSRQIQVLPREGGFQARLRYES
                     LQIDVDPVPTEDQVLLGLIHLLHDRGYRQLRTQRIFIGEQYLGNQEMWVEYPDPEAPP
                     ETRNTWVAWLRRILGRSSSPS"
     gene            complement(33504..34880)
                     /locus_tag="PKM72_00740"
     CDS             complement(33504..34880)
                     /locus_tag="PKM72_00740"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNP59328.1"
                     /translation="MAFVPLHRTALRLGLYIKIEGSWFSHPFPTNSFKIELTKDLETI
                     RELTNVKLYFDPDRSDPEMAHSNCTEKPDRVQVNNIKKPGGKSSDESEAYLTTDSSEE
                     DAVPENPIQRKIAQHEAFQVYQEHLQAVGGQFQEVVQEAKQVMQDVISGRPRGLRTAE
                     KIVGELYEILDIADNSRALLNLIGSNEPNEEFFLHALNVCSLSLMVGQDLGFSREDAE
                     KLALGALFHDIGELRFSAEQLLRKGSMSAFERKNFLSTHPKFGVEMVEKLPNFPYEAI
                     DVICRHHERLNGSGFPAGKKDPQISMFAKIVMVVDEYDELCHHPDPTKSLVPSEALSY
                     LYVKCRHTLWQDAVVSLISQLGVYPPGSLVNLSNQKIGIVTSVNLANRLRPVILVYDE
                     SNSSNEPIILNLSEEDESLSIVSAIRPVDLSPRIRECLNPRRIISYFPSASPAESRVG
                     ALNSLAGS"
     gene            complement(35349..36767)
                     /locus_tag="PKM72_00745"
     CDS             complement(35349..36767)
                     /locus_tag="PKM72_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP59329.1"
                     /translation="MSIIADTLQRLQTQTKREGTETTQQAAPLVLPARVRREPGWHRR
                     PSPLKFWLIGIGMAIGLSSLGLGAYWIGLHLDFGMPTEASPFPSQHVALSNFSPLAET
                     PPADSVSFETSQIPTADPVESLPISATQEPDGESPTSQESISSQLPSPLNVKHALLTP
                     PVGEIPASSTPETPVVTPTFNDPQQKTVPTSLSMPPQTPDPSPKHQSTDPKKTGTSSP
                     IPIVAQTEQSDSDLAESTSVNETDIAIPLEVDLEEEKIILGEFANTTAPVNDEPSIPS
                     NTIALPLKNEGTQENGQVTDPAQRSPANRLHHVQELIQTGKYEEAQAILSPLFHDPPV
                     TWEPWFWMGTAYLGTGQLDQADQYFLSGLARNDKVPQLWIQRALVAQQQGSFQLAVHE
                     LRQAEALQPDLPHIHLNMGYVYERMGNNRLANKYFGRFLQLTEGQPEFFPIRKKLFAS
                     LTSMTKTQISTPDKPLSLRGER"
     gene            complement(36796..37620)
                     /locus_tag="PKM72_00750"
     CDS             complement(36796..37620)
                     /locus_tag="PKM72_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP59330.1"
                     /translation="MTTLDLSMCYQELGFSEPPFRLTPDTDYFFPGSHHLEALDHLRF
                     GIASGGLTMLTGEVGLGKTLLCRHLLRHPPTGVRFAYLMNPDQSYSDLLVSIYEDLTG
                     TLPEDQSLGALQRELPKLLLRLAVEGERVVVLVDEAHRLSGKVLEGLRLLSNLDTEKD
                     KLMCLLLVGQPELEQKLATRAFRPLRQRISVRYRLESFTWQETRAYIRHRLHIADSRH
                     RFHLGAGVLWLIYAWSRGVPRRINQLCDRALLAAYAQGRHTVTPMMIWRATKEFLP"
     gene            complement(37620..39233)
                     /locus_tag="PKM72_00755"
     CDS             complement(37620..39233)
                     /locus_tag="PKM72_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNP59331.1"
                     /translation="MRHNTIVNPQTIIISNRPSNGASITYGVRVFQSLALLGMLSLWG
                     CTIANPDADRSISDANTTQTSQSLSLSSDLAASSVNNLRATFSLEDRMLLTSDLPKPP
                     TPPPAPSLGPLYSFRAQDLPVIDALALFARSNHLNIVTGPDITGTITVDFHHLPLEQA
                     MSAILEAHGYYWEHHQNLIQVRQFKTKTLNVDYIRLVRSGTGQNRAQLSSGSSGGSGG
                     GASQDTGQITVNQEDEIKFWEELEMQIKTLMSEEGRLVVNRLSGTIQISDRYKRVDEI
                     DDFLKSVHQALYRQVEIEVRIYEVALDDQYSLGIDWSRINFDGTNGAIALANIITAPF
                     GGFMAKAATTTISFEDGSFDGVLEALQEQGDIRVISQPRVVTMNNQPALIKVATDEPF
                     FSSTVAQGTAGSGNIVTEQVRSVTVGLVLSVTPQISEDGWIMLDVTPILSRLREIRES
                     PGAKRGDGGGATAPVLDVKQSSGLVRLKDGEMVIIGGLIQEESSETERKVPLLGDIPY
                     LGRLFKGTYTAKKKSELVIFLSPKIIRAS"
     gene            complement(39262..39777)
                     /locus_tag="PKM72_00760"
     CDS             complement(39262..39777)
                     /locus_tag="PKM72_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59332.1"
                     /translation="MDENQRFRRALKNPFVAIGLCLLAGFAVYTNFVDTASDAPGILS
                     VGLNRLLSAPTLPPLTPTGQPHDDEAGQWIEYSSRDPFAPFVDASHAQSDPPSMGADQ
                     TEEKQVTNTFTLKAIAVEGEQRSAVINRTVVYEGEMINGYQVLSILPKGVWLKHRGKN
                     QWLTFPKKATS"
     gene            complement(39744..40331)
                     /locus_tag="PKM72_00765"
     CDS             complement(39744..40331)
                     /locus_tag="PKM72_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59333.1"
                     /translation="MKWLPRFEWPKPQQLKSTALVVLLFGTLSIVGWVSLDKLFTIQK
                     RQSLTQDTIGLEQQVSELRSQYLQARPDALDTHIQLAEQRLLHNFTHLAQWAQTLQFD
                     GKQRNLLIQYRILSTDRTTTPIQGVTLVPLEIQVNSQGTPHAYHSYLQFLKALTRSGP
                     RVDIQNVTVMGDGQQATHLTIGLSVWMKTNDSVEL"
     gene            complement(40328..41824)
                     /locus_tag="PKM72_00770"
     CDS             complement(40328..41824)
                     /locus_tag="PKM72_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59334.1"
                     /translation="MAPKTMIDTLQHPTSYSEVQTPILEIPPNLRKTLLRKKQTILSV
                     SLSNGRLKALSIVKNTIGQSWEQPGRLVSLHTLRDALEEAIHHTQFPGTHLAMLVDHP
                     RLATRTIQIPPMLPTDLLPFLERKVQQEKPWEGPAAWRYRVGLEARGKLHIHLDIWPQ
                     DYIDKIVHICQELNLSLRQLAPLSTLSESQLSALPVEPGKGSMLITMIEGKIMIVAGN
                     DDGTLIWTRHLFPAQDWVPLGERVGTEANRTIMFITQQTNLKFPCIWFWDDEERLTAG
                     EIQPHLSTPLVPFPIKPDWNYWLWVGAMLPVNLSSNFTPTQVLQAPLRKTLTKAFVSM
                     LAVFILFGVGTTSVIEGYLARHRTAMQTMTDQVTSLQQDYAQWESRLLSLDTKRQWAH
                     SVSETTTPELEGPFLSYLGSIIPTQVILRKAFVERTPDGWNVELDGSTSTNLAATLLV
                     VDQFVQKLADGPYHVSLHKDWRDQLLTQTATPTNERPLYRFTLKGTIS"
     gene            complement(41814..42734)
                     /locus_tag="PKM72_00775"
     CDS             complement(41814..42734)
                     /locus_tag="PKM72_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNP59335.1"
                     /translation="MTRRNDFLTQAIGRHTASISFKTDWRPGTTLQPSQCSNRNAVEI
                     IGRRMSAFIRTSRCLATSGFTLVEMIGVMAIIAILASFIAPNVINQLASVRRDSEDGQ
                     LATIAQGVELYVRQTHSFPTTLTALSPDYVAMALGQLTTNPNGFLRYYFVQPNISGFT
                     NSTGLPTTALADARFMVITHLAQNVNPSITTDAHFETWWSTDETGTPDLKIHRGHVSH
                     LFHLLSLSANGAGGSYAIDGSQTVAGVGTLVPHIRYHLTGTPISLDETNSFTGQKTQF
                     TLTGEAGFQFDPDCPAGSQWRLNSSGCYGP"
     gene            complement(43012..43806)
                     /locus_tag="PKM72_00780"
     CDS             complement(43012..43806)
                     /locus_tag="PKM72_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNP59336.1"
                     /translation="MNSPGVSARSSTHHQITSDPEAGMTLIVLIGLLAMISIGLAVVS
                     PSMVRVWDQNSEDRETRDLQMIATGVIAYLRQHHTFPPSLLALSPDYVPLPLAQLTRN
                     ARGFPRYYSVHPSIAGFQNSTGLTGAEIINARFLLLSNLSQDAAPSITTPVSFETWWN
                     TNDSITPALTIHRGTISHLFYSVSLIPKGNGGSFSIDHQPTHSNGDLLPTHSRFHLIG
                     TEIGMDEDDSYNPPPDVQFALTTNTSYWFHPLCVFTKRWNPLSPPC"
     gene            complement(43815..45026)
                     /locus_tag="PKM72_00785"
     CDS             complement(43815..45026)
                     /locus_tag="PKM72_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNP59337.1"
                     /translation="MANFQYRAIDDHGKPVEGEMTAPDLFHLERQLQDMGLWLVRTFE
                     SKPKSLARTTQKRGRVKPRDLMEFSTHMFTLLEAGIPLTQALKSLSIETPNLHLRATL
                     QAIFQQVEAGNTLHDAMKQHPRIFPPQITNLVHAGEDSGTLTDTFKELERYLDWLDQI
                     RGDVRQATIYPSIVIIAVIGLVVLLFTFVIPRFVPILEGLHVPLPTITLIIITLSELF
                     VGSWWLWSSFLLLGPMVWVLGQKYLPEFAHWRDRMILQLPVLGELIRMLTISKFVQNF
                     AVLYRAGIPVLQSLQLCQGLVGNSVMERALQDTQRAVSEGSTINESLNRHPIFPPMVI
                     QMISVGEATGTLPNTLMNVANYYNREIPRRIKKVFGILEPVITLGLIGIVGIVALALF
                     LPMMSLMGGIH"
     gene            complement(45019..>45896)
                     /locus_tag="PKM72_00790"
     CDS             complement(45019..>45896)
                     /locus_tag="PKM72_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNP59338.1"
                     /translation="PTNFGESIVLRILDKGALKLEFLALGFTPNDQSRVQALIQRPHG
                     IILVTGPTGSGKTTTLYTALRHVDAKEKSVFTLEDPIEYQMPLIRQTQVNPDIGMTFA
                     AGLRALLRQDPDVILVGEIRDQETADLAMRAALTGHLVFSTLHTNDALGAIPRLIDMG
                     VEAFLLASALTAIIGQRLVRCICPECKVERTDASTILADLRVELPEGPLLSLWTGKGC
                     HACSQTGYKGRAGIYEIIPISEELHAPIVHGPDMAGIRAIIRRDQMPTMFDDGLLKAL
                     QGITTIEEVLRVTGG"
BASE COUNT        10805 a        10509 c        12360 g        12222 t
ORIGIN      
        1 tagtcatgga ttctggagaa gggcgtctcg tggctgggca ccgttttggg agcttcactc
       61 catcatttga tggtgtgcaa ttgacccttg gtggggatga aagctctggt tggggacttc
      121 gggcctttgt cacgcaaccg gttcaacgtc atacggttag accggattgg actagcccca
      181 ttaactactt ttccggtgcg gcgatctcaa gccgccatgt cccgtgggct aatggtgagt
      241 tatatttgaa tgagttccat tatccttcca aagggaaaga ggcctctttc tcctgatcct
      301 gccggaacaa gattgaattt cgcacagggc acaccgtacg aaatcgcaca gaggagcttg
      361 ccgggaaaag ggttgctaag tcggccagga aaaaccttgg cagaataatt tgaaatgtaa
      421 aggaaagtca gaaagttttc tttgggacgg acctttttgt ttaatggcat gtaagttgcc
      481 gatgatgctt gtggggtaat ggaaaagcat gtcattggga ttttggatag tcaatgaggg
      541 actaggcgga ggcaagagga atggaccacg tgactgcggg tattcgtgaa tatttcaaag
      601 attttaattt caaagaaaac ttcaaaggga ttaccagcaa tgtgagaggg gatgtgttgg
      661 cggggatcac ggtggcgatg gtggtgttgc ccatggcctt ggccttcggt gtggcttccg
      721 ggctcggggc gattgctggc atgtggtcgg cagtggcggc tggtcttatt gcgggtccat
      781 taagtgggtc agcatggtcg gtgggtgggc cgaccgggcc gctgacgatc caaatccttt
      841 ccatgactca aaccaataag ttggctgatg ggagtccaga tttggttttc gttttcacga
      901 ccgttgcctt agcgggcttt cttttaattg tgatgggctt gttgaagctc gggcagttta
      961 tcaagtttac cccctattca gtcatttcgg gatttatgac gggtttgggt gtgttgtaca
     1021 tgttacttca gcttaacccc tttttgggac ttcccggcgt caaaagtatc acgtccgcca
     1081 ttacggaatt accatccagt ttggcccatg catcgcctgt ggcgatggga gtcggggtgt
     1141 tgaccttact catcgtggtg tattggccaa aaatttctcc cgtgacctgg ttgcctggtc
     1201 cattgattgg attatgggca ggtacggtca caaccgtcgt gttcgggttg gacatcccga
     1261 aaataggaga tatccccacc ggcttacctg aactgtatat tcctgatctc acgctactgg
     1321 aaaaagcttt tgtcccggct gcggctttgg ctggattatg tgtattcgat agtctcctga
     1381 cctgtctcat cgtagataat atgaccggca ctcatcacaa cagtgaccgg gagttggttg
     1441 ctcagggatc agccaatctc ttctcaggtt tggttggagg attaggtgga gccacgaata
     1501 ccatgccttg tgtcgtgaat atccagagcg gggcacgaac caggttgtcc tctgtcacaa
     1561 tgggacttgt cctgctttcc tttattctcg gtctgggttc tctggctgcc tccatccctc
     1621 tttccgcttt ggcgggaatc ctcctcaagg ccggctatga cattctggat atgcgagtgc
     1681 tccctgtcgt cagaagattg cccacgtctg atttgatggt gtttggactc gtggtcttaa
     1741 tgaccgtatt ttggaatctg ctgtccgcca tggcaatggg actcgcagtg gcctttttcc
     1801 gtttcgtgaa ggatcagtcc gatcgctata aggcggatct cagccaacgc gatgaggatg
     1861 taaaacagga agaagaagac ctcatttttt ccttcgccag ggactatgcc aggaagatca
     1921 gtcagaatgg ggtcaatata ggcgaactta gcggtcgttt tgaacatatc gttcgggatc
     1981 ggcttatcat cgtacgtccc cacggtccct tatttttcgg tgcgattgat tggctgaatg
     2041 agacggtgga acatcttgac ggtaaagatg tgctcattat tcgttgcaaa tggttggatg
     2101 aattggattt atccggtgcc tatgccttag gagatttaat tgaagcggcc aactgtcgcg
     2161 gggtggcggt gttgaccgct ggcatgtcgc ctcgaacccg acaggttctt gccgatttaa
     2221 atgagctttc cagattgaaa gacgattata tttgcacaca tttcaatgag gccctggata
     2281 ttgcgatgca gatcgtggaa aagaaggcag ttgacgctgg gccacaggcc cacaaagaac
     2341 cagtactggc attcagttag aaaacggtta tgttctctcc ccaacaaacc gggcgagata
     2401 tgtaaatatg acattccgct tcctttgtgt atttgaacaa agttttctcg accatgcagc
     2461 gaagtggtat aaggggattt ttttgaaagt gtctcatcag gttcattgta cggcttttcc
     2521 cgccatttat tttcgtttgt gatcacagac tgttccaatc cgaccatgcc atttttattc
     2581 gttaattgga gtccgccgat ctgtcaactt gttcagaaaa tagactctgt atacgggaat
     2641 tatcctgagc tggacagatc tcataatgag attagacagg ctattggggt atagtggatt
     2701 gggctctgat gaggtaggac aggtctgtta ggggagaagg cttgaaggga ccgcaagatg
     2761 ggttttgagg attaccaatg tctctcaata aattattttt aaatcttccg attgccaaga
     2821 aactcctttt agtctcgggt gtcccggttt tggccgtctt aattttgagc ctggtgacct
     2881 atagaagtgt gcagactttt tcgctggatg aagaccgcct caatgatgtg tatcatgtgc
     2941 aaagcgcgtc atctgaatat atgcgattgg ttgtggatct cgaaaccgga tttcgcggtt
     3001 ttattctgac ccaacaaact ccgtttttac agccctatct ggcagccaga caacgggtga
     3061 tgttattggg gaattccttg agacaaatgg tggagcccat tgaggaccaa cgacagttaa
     3121 tagaatctgt tcaaaaaatg atcggccaat taatgaacga caaagatcgg attattgaac
     3181 gggtcaaagc ggggcacccg gaggaagcca tgaactatgt ggagagcgag aaaggtcgta
     3241 cgctcatgct cgcgattcgt gaagagatgg ctcgctttga tcggcgggaa gtcatgcaac
     3301 tgcaaaaggc acttgggagt agcgccgcag accgggccgc cctgatggga gtggtcgtgt
     3361 ggggagggtc gttggcgttg attttattgt tggtccctct tcatctcatt gcgcgttcta
     3421 ttactggtcc tttaaccacg ttggtcaagg ccgtggaaaa tgtttccggt gggaccatcc
     3481 cgagtattcc agttttccaa agagacgatg aaattggcga attgacgaaa gtcatgcaga
     3541 caatgggcac tcaaatccga aatcatatcc gccgtatcga acagtcggaa caggaactcc
     3601 gaacgctgaa ccatgattta acctcttcgg aggccaaata tcgaggaata gtggattatg
     3661 cgcctatcgg gatattcacg gcaaaagaag tccacctgat ttttagcaac cggcagaatt
     3721 ggattctagc cggcaggagt tccgatgagg tgttggatcc cgaacacatg tgggaggcca
     3781 ttcatccgga tgatcgggag ggtgtgcgcg agacgtttca agatgcctgt catcaggggg
     3841 agccttttga acgggtcttt cgctttctgc atccggatgg aactgttcga aaggtcttat
     3901 gtcgggcggt accgattcag gacgaaggtg gtcagacgat ggtataccaa gggtttaacg
     3961 tggatatcac ggctttagag catatgcgtg caaagctgaa ccgatctgaa cgattggcga
     4021 cgttgggcca aatggctgca ggtattgccc atgagattcg gaatccgctt gtgggtattg
     4081 ggtcaaccac gacgttgctg atggatgatt ttcctgaggg tgattcacgg catgatgatc
     4141 ttgtgacgat tctcaaggaa acccgtcgat tggaccgcat tgtaaatcaa atcgttgact
     4201 ttgcgcggcc aagagatttg cttcccgtga cctttcaact gaaagatctg gttctggaat
     4261 ctttgcaggt gctcaatgaa ccaataaaac aaaagaacat tcaggtcgaa tttcatccgt
     4321 cagccaattt ggacaccatt caggctgatc gagatcaact caagcaagtg ttgctcaatg
     4381 tgattcaaaa tgccgttgag gcaatgtcgg atgggggaag tctttgtctt tccattgaag
     4441 aggaaagcat cgaacggaag cctgggcttc gtcttaaagt taaagataat ggaacgggaa
     4501 tcagtccagg cgcccttcct cgaattttcg agccattttt cacaattggc aaaaatcgcg
     4561 gtaccggatt gggattgcct atttgtcgga atattatcga agcccatgga ggcgaaattc
     4621 atgcagaaag tcagtcagga ctggggacca ctatgacatg gtggcttcct tgcgtttgcc
     4681 aaccccaact cccaacggtg taaccaatgc gcgccaatat ttttgtcacg gatgatgatg
     4741 atgtagttcg ccaggccatc agccgacggc tcgcgaagcg acaccatcaa gttcggagtt
     4801 ttgcgtcggg agaagcctta ttggaagctc ttgaccatga tatccctgat ctgattttgt
     4861 tggacttgaa aatggccgga ttaagcgggc tggagactct caagaatatt cgtgccaaat
     4921 ctcagcaagc cttggtcatt ctccttacgg cctatggcac ggttgaagag gcagtggaag
     4981 ccattaaact cggggcctat gattttctga ttaaaagcgt cgatttctcc agcgtggagc
     5041 ccgtgatcga tcgagcattg gactatctga gtctccgtcg ccgtatcgac tttgaaacgc
     5101 aggataggcc aggccgatat gccttaaacg gtttgatttc caatagcccc agtatgaaag
     5161 aattggttgg acaaattcag gaaatggcca aaaatctgaa aacgaccgtg ctcctctttg
     5221 gagaaaccgg aacaggtaaa gaatttgtcg cacgagtgct ccaccataat ggtcctcggg
     5281 acaaaggtcc gtttgtggga gtgaactgta cagccattcc tcaggaatta tttgaaagtg
     5341 agttgtttgg ctatgaacgc ggggctttca cgggggcaaa ccagcgcaaa ctcggcctct
     5401 gtgagcaggc cgaaggcggg actctttttt tagatgagat tggagacctc aatccctcaa
     5461 tgcaagccaa attgcttcga gttttacagg aacgttcgtt taagcggttg ggggggcaag
     5521 aagatattga tgtggatttt cgtctgattg cggccaccaa tcgggatttg cgcaaagatg
     5581 tgacgcaagg cagatttcgg gaggacttgt tttttcggct gaatgtggtg tcaatgactc
     5641 tcccaccatt acggaaccga acagaggata ttatcccgct ggctctagcg acgttaattc
     5701 gacaaagcag cgaacttggt aaagaggtca cggcgattga gccagaggcc caacggctgt
     5761 tggaacgcta tccgtatccg ggaaatattc gtgaactcga aaatattatg gagcgtgcga
     5821 ctattttttg tcgtgggaaa agcttgacgg aaggggatct gcctcgtgaa gttcatgaag
     5881 aggcacgttc ctcagtcaat gcggtcgctc gaggggatca gcaggttgtt cgcatggaaa
     5941 tgattcttgg ggaacaaacc cttgcggcca ttgaaagtga tttaattgag gaagtcatgc
     6001 gattgtctga ttataataaa agtctcgctg ccaaatatct tggcattacc cgctttgctt
     6061 tggaccgccg cctcaaaaaa attcccgagt cttgatcccc cctgttgtta cctgaggtag
     6121 cccttccatg atgatggtcg agtgtttgtc ttccaagaac gtgtagctgt cctgaagtgt
     6181 ttcttcagga caattggata ccatctttgt tcacagagaa aaggggccat ccctaagatg
     6241 atttttttcg attttttaga agaacaacga tgccaatgat tccaatcaac agagccaatc
     6301 ctcccatctt gctattctcc ttttctgatt ccgttgcttc tctgtttttt ggctcgctat
     6361 ctgtgtcttg tcgggcaaag aactgtgtcc cgaccaattc ttaactaact tgttcctatc
     6421 cttcctgttt ttatgggaat cgttaaggtg tctttttaag caagtatgct ggatgccatg
     6481 agtgatggaa tccggcagcc agatttattg ctaaggaaca tgcgtcacag tattcagatg
     6541 atgagcgcgc aacgcggttg aaaaaacttt ggagaccaag tgtatccttg ctcgtggtcc
     6601 atatgttgtt tattagtctg aaggcttgag gttgaaaagc gttgtggcta aagcaaaccg
     6661 ctggtagaat tgggtgctca tgaaaatcgc catcactcat gcttgctgac gtggtcctgc
     6721 cgggcaggcg ttttcaggtc ttcacttatc aagtcccccc ccatctgctt tccctgcttc
     6781 atgtgggaag ctccgtggtc gtgcctttag gctctgctgt cgtttctgga gtggtggtca
     6841 ccatctttga ggccaaaagc tcttcttccc tccaatctca attccgtcat aaaccccttc
     6901 gcgacattct ttcgttggag ggcgatgtcg ggagcagtcc tttggaacac aaccttattc
     6961 gtttggttga aaaaatttca gattattatc tggctccttt ttccgcatgt ttgcggctga
     7021 ttgttccccc acattcgata caagtgataa ggcgtttaat cctaaccgac gatggtcgtc
     7081 aggctttgtt ggacaggtcg ttgccatctg atgtccagtt ggtccttcga aagttagagc
     7141 gggctcccag gggattactc cgatcctctc ttatacgaac aatgaacaat gttccagctt
     7201 ttctgactcg tgcgaaaaag aaaggatgga ttactgaaca gacgattatg tcctcgggat
     7261 ccaagggtcc aagtccggtc cggggaatca gtgccggacg aaagccggtg ccctctatta
     7321 tccgagggtt atttgattct cctgaagaag atgctaggga gctgccggaa tctgcccgcc
     7381 gaatggagtc cagctccttt cacgacaacc ggatatggaa gaatctggtg tctgctgtgc
     7441 agtccggtgg ttttcaggag gtgcctgttg tgggatcaga atccgtccga caggatctgc
     7501 tattgaagct gattcagacc attatagatc agggacgtcg agtgctcatt ctcgctcccg
     7561 aagtgcagga agtcgaaatc cttggggaac aggttcgatt ccgggggagg attcaggttg
     7621 agatctatca tggccatctt tcgacaatcg ttcgtgctgc tcgatgggaa cggattcgac
     7681 agggtgatgt gcaggtcgtg gtggggactc gatctgcgct attcctgcct attccaaatt
     7741 tgaggctcat ttgggtcaat caagaggaga gttcgtcctt taaggatgag catcttccgt
     7801 attttcatgc tcgggacgtt gcgcgaatgc gaggcgattg tgagcaggcg ctcgtggtct
     7861 atggttcgac gcggccttct ttggaactat atggacgttt ccccgagcag gtcggtgagg
     7921 cactggaagg cccatcacag cagattcctc aggtggaaat ggtcgattta cgcactttac
     7981 cttatgagac cattctatct ccggttttac tttccaggat gactcagacc ttgaaggagg
     8041 gcgagcaact tattttactt ctcaatcgca aggggttttc cggtgccctg gtgtgccgag
     8101 attgtgggaa gactccgatc tgtcccactt gcggagttcc aatgaaactc tatcagcgtc
     8161 cttcacgttt ggtgtgtacc tactgtgatg gaatcggacc aactcctgaa atctgtccca
     8221 cttgccatgg cagggtgttt cgattttctg gcacgggaac ccagcgattg gaggatgagg
     8281 tgacccgtct ttttccggat gtttctgtcg cccgattcga tcgggagaat gtgaaaactc
     8341 ccgaagtggc cgatgcgatc ttgcgccaat tccggcaacg aaagattgga gtattgattg
     8401 ggacggaatt tttggttcat cagtcaaatc cacccatggc tccggtgatt gggttccctc
     8461 aagccgatct tgggctccat attcctgact ttcgatcagc cgaacgaacg tttcagatgc
     8521 tgtcgagggc tttcaccttg gcccggaatg ggcaggagcc aggggaagtg attctacaaa
     8581 ccagaatccc ggatcatcat gtacatactg ctattgggca ccatcgtccc agaatgtttt
     8641 atgaccacga actgcagttg cgtaacctac taggatatcc cccggctacc catgtgatct
     8701 tggtggttgt aactggggaa cagaagccac gcgttcaaag ggttgtggat tttctgcatc
     8761 aacgattgaa agcttttgaa gcgagaagag ttccttgtgc agaggggaag gggatcatcg
     8821 gagcatcaat gattttgggg ccaatggtat ctaaaaaacc cgggcggatt aaaaaaaatc
     8881 gcgtactttt tttgatcaaa acggatgatt tatcggaagc acagcgaggc cttcgagcca
     8941 tacagctgga gtatgaagcg gaatttcaca aagatcctgt gatcgttgaa ttcaatgttg
     9001 accccatgga catccagtag caacgggttg gtctcatacg cgtgaggatt gcctccttga
     9061 ctgtttcttc tggttttttg ttttgggaac ggtgtttgaa ggggcgggcg gggagttttt
     9121 atctcgagtg cgtttgaaaa taccataggc gaaggtcatc cagaacacgc cggaaaatag
     9181 gccaaacaac acggcggtaa aaattcgttc caattcttct aatggaggtt ccggtcccag
     9241 cttttgaagg tcggccattt gcatgatggg ccatgccaga ctttctactc ctaagaggag
     9301 cgtggaggtc gcccagatta atccaataga cggacatttc caggccaaca ggatagccag
     9361 cccgatggcc agagcaattc cgatgggtac cggtaaagcc cccactaata accagagtcc
     9421 caccgtaatt gtgaggctac ccaaaacagc gttcagttga tgccacaaat gatcattcat
     9481 ctcgaatcgt cctagaaatt cctgtcaacg ctgcccacct taccatagac tttcgttgcc
     9541 cagcatcttg tattgtcctg gtggatgatc atgcatgcgg agattggatc tagcgaccaa
     9601 tatcattcaa gggttcagtg gtgagggaat aggccccgcg agggttattt tccaggtggt
     9661 tggattggtg gagctcttca ggtgttggcg tggtttcaca gtccggagta agcacgattc
     9721 cccaatgcgc attttctgca caggtattgt cgaggataag cgctttggcg aggtgaaaga
     9781 ggagaatgcc actgagcata tttccgcgac agatgttttt aatcatatcg ggtcgagtgc
     9841 cctcgtcccg tgcggctatt ccaaaatgat gatttccaaa acacgcattt tcgcgcaaat
     9901 aaggctgagc tccggcaaaa gaaaaaatac cggactcccg attatgagaa acctcgttgg
     9961 cgagcaaggt cgggcggcaa tccggtccat aaatcacgac tccggataac agtccttccg
    10021 tggccctgca gtgagatatc gtgcataccg aatctaaaat attcagtgcg gaatgttggt
    10081 cgctgcccac gtaccgaaac gttatgccgg taatgtttcc tccagagact cgctgaaggt
    10141 agcaagggcc gcttcgtcga ttgaatattg tgaccaggtc ccggccggcg ccgacaagat
    10201 ggatggttcg ctcagtcatt accaaccgat cttcatatac ccccggtcga atgaatattt
    10261 gatcatcttt ttgggcttga gcgagagctt cgctgggtaa agtgaagcta cctgggcgct
    10321 tcgcgtccac aattaacgtt tttcccccga atggattggg cggtggttcg tgaaaggatg
    10381 atggttcggc ggacaaggct ttctcctctt tcaaaagaat tctggcaaat ggcgaccctg
    10441 atatctacgg gaccaaaaat gtatcgaccg ttcatcaagt atgcgaatgg aaaccgaagg
    10501 gtgaaccata cggaatttcg aagaatgaca tttcacaatc ttaaatccaa tccaattcac
    10561 cgacgtaaag ttctgtaatg ttggtatttt atatcctggt attgaatttg cttgagatct
    10621 tttccgttgt acaaaaaata atcagtaaag tcttttttgt ctaaatttat tttttaaggg
    10681 gtgaaaaatt ttttcccatg ttggggagca gtccgctgcc gccctgatta actttaattg
    10741 tgtctgtcag agaagtccgc ctccccctcc ccctcattaa tttcaacagt ttcccccagg
    10801 aaagagagtt tcgttatgtc aaagaatggg agaagtggta tgtctctgtt ctttttgttc
    10861 ctagtgctgg gcacaatgtc gtttgtaaaa ggtttatctg gagcgcaggc acaggaaacc
    10921 gacatctttc aacatcctaa ttctgaacag gcagggatta agaaatcttt ggagcagcaa
    10981 attggggctg gccggggaga tatcaacact cctgattcct cgctatatat tattaacagg
    11041 gatccgttcc gctcgattcg tcgtggtcga aatctttttc aacgcaaatt caccatatcc
    11101 caagggatgg gcccacgcaa tggtgatggg tttggagata taagcgccga tgcctcgctg
    11161 ggtgcaggtt tagcggatag ttgtgcggcc tgccacggac gtccacgtag ctcggcagga
    11221 tttggtgggg acgtgtttac ccgtccggat agtcgggatg ccccgcattt attcgggtta
    11281 gggcttattg aaatgcttgc ggatgaaatt acccaagatc ttcggaaaat ccgggatcag
    11341 gccattgcca gagctaaagg aggacgaggt gtggaggtgt cgttgaagag taaaggcatt
    11401 tcctatggaa aaatcagagt caatcctgac ggttcggtcg atccctcaca cctggaaggt
    11461 gtcgatccgg atttgcgggt acggccattt tttcatcacg gtggcaccat ttccatcagg
    11521 gaatttgtgg tgggggcatt gaatggggaa atgggtatgg aggcttttga tcccgatctg
    11581 gcgaaggctg ctgatggtga ggaagtggtc acaccatcgg gaatggtgtt aaacggaaag
    11641 actgatacca tcgaaacgcc tccggctttc agtgaaagtg atgattcgga tcgagatggg
    11701 gttgccaatg aagtgcccgt gagcctggtg gaccatcttg agttctattt attgaattat
    11761 tttaagcccg ccatcgggcg tgagacgaaa gatagcaaga gggggcagag gctctttcaa
    11821 gacattcaat gcaccagctg tcatatccaa aatttagtca ttgagaaaga tcgtcgtgtg
    11881 gctgatgtgg aaaccactta tgaccgtcga cgagggattt tcaatgatct tttcgccgtg
    11941 gccactcctt tgttccaaga agtccagggt tcgggttctc cttcggtgaa aaaacccaaa
    12001 ggtcagcgct ttgtcgtcaa aaatatctat gcggatttca aacgccatga cttaggctca
    12061 acctattggg agcgcaattt tgatggttct ttgactaagg agtttatgac cgaaccacta
    12121 tggggagtag gtacaactgc tccttacggt catgacgggc ggagtatcga tctatggtcg
    12181 gtcattatgc ggcatggagg agaggcgaaa gatgcacggg atcgctttgc ccgattatcg
    12241 gaggagcagc aagggcagat tatcgatttt ctacagactc tggttttgtt tccaccagac
    12301 gataccgctt ccaatttaaa tcctggcgat tctcaagccc agaattttcc gcaatatgga
    12361 catggtaata tagccctgcc cgcgttattc aacaatccta gcgatgtgga ataaatgaag
    12421 ttttctttat tcttatctgc ctaatggaat caagcccgtg atccgtaagg tcacgggctt
    12481 gaaccctctc tcgcaattgg ggatgtttcc cattgtcttt ctcttttttg tgcgatccaa
    12541 catcaagcgg gaaaaaaacc atctggattt caaccaggtg gcttctcacg cctcccttgt
    12601 ctctcgccgc atctctcgtt gctgtgcgtt ctagccctat cctgatggac tatttttaga
    12661 attatcgggt ggcgaaaatc acgtcacgta tcgatttgaa attaaaagaa gacatttctt
    12721 gtcttcaaga atgttcagag acctgggagg aaaatgggtt aattattctc aaatgcttgg
    12781 cgggttgcga agacctcgcc tggatgaatc aggagcaggc aatcgccatc aaccggatcc
    12841 ttatgaaaga gtgcgaaggt gaggcagagc tgaggggtcg gttcaggaga aaactgtaac
    12901 aagatgggaa catccaccag gggagttggt gttttcagga tggccaaccg gaggcggaca
    12961 gccaccagcg tatcgcgtag gcgtgtttga ataagttctt caggataatc tggagaaggt
    13021 gtaatgtatt tgtcccagag cggattggtc atgcccacac agaaatccag ccctacgctt
    13081 tgcgcaatag tcgtaacatc tttgagtatc ccagattcaa tggcttcttt gcgggatcca
    13141 atcgggagtg attcaaattc tgaagggata tctcctgagc ccagggaaag agctaactgg
    13201 gatgattcag agaggtttga aggtaaaagg ttagatctat cctgggagtc ggtgtcgtct
    13261 ttttctaaag gagcaagatc gctcaaaggt gtcttcccta atgcttgaag aagaccttca
    13321 tagacggctt tggcggaatc cgtccatgaa tcattcatcg gcagtccccg aaaggccgcc
    13381 tcggaggctt ttttctcatc atgggttaac atgcgttctg gattcatacc ttcaaagata
    13441 agctaaattc ctgagaagtc aagagtaaaa catttctgtc tccaaataag ccaggaatgg
    13501 aaacgcagaa agagtcgtga attcagtcaa gaaagcggtc caattagccc agccatttac
    13561 agatagcaag gtggttgatc tctcaatcga tggttttcag cgaaaggttg cctccaaatc
    13621 ccgatctaaa gtaaggacga tgatgcgttc ttcgattcgg gtatcgatat ctgtaaataa
    13681 agcagtcgtt tccgctccgg tgatttccaa catttcccga cacagttgct ctcttccttg
    13741 ggaaatgaga ttttggcgca tctgcttgac cattccaatg ccttcttcac tcttggccag
    13801 ctggcgttca gctggcgtaa gaacgccctt gagccgaatg agaatggtgt cacggagtat
    13861 agaagctcga acatcggtgg ggccgcggcc catgaattcc tgctcaaatt taataatggc
    13921 atttcgaatt gccgattcca tttcccctcg tgtgacagtg tgtttcatgc ggtcatgctt
    13981 tctggcaaga gcgatcttcc ccttcaaaat cggatgtttg cgcattcccg aagaaaatca
    14041 cggaaacttg aggaacaacc tgtccccctg atataagcgt aaaactgatg tgaggtcaag
    14101 aagaggaagg gcttcttctc gaatgttcga gcagagtggt ctctgtgctg tttttgaatt
    14161 tgaccgtaga acgggtttgt cggatcgcct gcatcattca ccatgtgata gtgaaaagcg
    14221 atagaataag ccaatcgagt gaactcctga agacagtaaa agggggcgtc cttcggttgg
    14281 cttttttatt gtcaattgag ttaatcgagt actgcacaat tgactgacat ctctcatgag
    14341 ttgggtgtat ggtcctgtca ggttttcccc tctgccaatt caccctgatt cccgttatct
    14401 tatccggtga gagcacaaat ggtttctcta tgacttgatt tgaggatggt aggcatccgt
    14461 gctctaaaaa aagtggaaat ggggcaatga acaaggttga gcaagatcct ttatccgtga
    14521 agagttggaa atggcttcga gacatcgttg atgaggcgtg tcagcccatc ggtccatatt
    14581 ggccgatgaa atcatttgcc tattataatc ccattcgaga cctcgagcat ttaccatttg
    14641 cccgtgcgat cgaggccgca aatcagttgt tgggtgccaa ggggtttctg cctgttggag
    14701 agtatcgcca actttttcat caaggtcgaa tcactctttc ggccattgac caggctcttc
    14761 ggcgcgtagg acctcccctg ccgtctcgaa ccactgtgca ggttggtgac cgtagcattc
    14821 atgtccaaga tgtctggcgc attcatgcta tatacgggat tgaaatttta cctgctgtgc
    14881 tgctgacgtg gacactggaa ctagaaggtc tgaacacacg atttcgatct gatcttccgg
    14941 agaattctcg aacatccatt attaaccgaa ctattcgaga gtgcgagaag tgccgacatg
    15001 atccggaatc ggcctatctg cacaacctct ggaaaagttc gttggcggcc tgccaattgt
    15061 tcgaccctgt gtcgggtgag ggttctttcc atgcatcgga ccttggtcgg agctcccatc
    15121 tatcaggaaa agaggcctat tctgtcacag ttgacttacc taaagaccga acgctgagtg
    15181 attggctgga cagtgagacg gggagcgcgc tggttgagac cattaatacg cacatgatca
    15241 aatggattgc ggcatttgta gacgaaggtg ttgcgggatg gagtatgcct tcaagagata
    15301 aggggttttt tgctgcatgg cgagaattgg ccgaagggga cctctcaggc aaatttctcg
    15361 ggattccgga tcttcgacaa aagtttcgcg aattatccga cgtaccggaa gagatgttgt
    15421 gcaaacatct gcagaatctc aatatcccga aggaacgttg gcaagagtat ctctgccgtc
    15481 atctggctca attgccaggt tgggcagggt ttattcgctg gcgtggagac catcctgggt
    15541 atcccgccca gcaacattac cccattgatc ctctgcaata tctggccgtc cggctctttt
    15601 atgaatcggg aatggtggag ggactttgcc aacgggaatg gggcatcaag gggacgttac
    15661 ctggactcct tgcctattgg aacaaacagg gtacgcatga acaggcgttg agtctctctt
    15721 cttctcacgc cactgttgct aatgatcagg cggtgtgcca tcaagcttgg cgtctttttc
    15781 acctggctca gtttttggaa ctgaccccaa ttgaggtaca cgaattgtct tatagcgaca
    15841 cgtcaacctt attggaatgg ttggatctct ttccccaatc ttcacatgga ccggtatggc
    15901 ttgaagcgta tgaggacgtc tatcgtgagt cattgctgag gaacatcagc ggacatcagg
    15961 ctgtggctcc cgttagcaat caacggcctc gggcgcaagg gattttttgt attgatgccc
    16021 gctcagaatc gtttcgtcgt catcttgaag ctcagggacc gtatgaaaca tttgggtatg
    16081 cgggattttt tggggtgccc atgagtcatg tggcatttga tagccacgac cacttagcat
    16141 tgtgtccaat tttgttgaca ccaaaagcgg aagtgatcga agttccccga atggggcagc
    16201 atgaaagggt gaagagctat ctttccggaa ccagatggca tcaattcagt catcacctct
    16261 ttcacgatct gaaacacaat ccatttgcct cctttatgct gattgatgtg ttaggaatgt
    16321 tttttagtgt tggattggtc ggaaaaacac tctttcgaac ctccttcgat gccatcaaac
    16381 agaggttgca ccagtggttg gggagcaccg ttgtcacgca cattcctgtt gagccttcag
    16441 gtgatgacga acataaaaat ccccctgtgg gagaactggc acaaggtttt accacattgg
    16501 agcaagcggc ctttgtggag ggtggattgc gtgtgatcgg gctcacgaaa aattttggcc
    16561 ggtttgtgat ggtttgcggt catggaagcc aatccgataa taatccctat tacgcggccc
    16621 tggactgtgg ggcatgtggg ggcagtcatg gtgacccgaa tgctcgtgcc ttttcggcca
    16681 tggccaataa tccagaggtc cgacagattc tgaacgatca tgggctggtt attccggagg
    16741 acacctggtt tcttcccgcc aaacataaca ccaccacaga tcaagtgatg ttgtatgacc
    16801 ttgtcgatgt tcctgctatc catttagagg aattaggact gttggtgaga gacttggagc
    16861 aggctggcac acatcaggca ttggaacgat gtggacgaat tcccggcgct ccgacagccg
    16921 tgtctcccaa tgaagcgttt aaacatgtca ggcaacggag catggattgg gcgaattctc
    16981 gtcctgaatg gggattgtca gggaacgctg cctttttgat tggaaggcgg gcccttacca
    17041 aggggctgga tttggaaggg agagtttttt tgcattccta cgatcccgga tccgatcttg
    17101 atggaagcct tcttgaaaaa atcatgaccg cacccctcat cgtgggtgaa ttgattagct
    17161 taacgtatta cttttccggg gtggatcctt gggcctatgg gagtggaagc aaggtcattc
    17221 acaatatggt tggcggagtt ggggtgatgt tggggagtca aagtgatctg caaaaaggcc
    17281 ttccccttca atcggtaaac gatggggcgc ggcattatca tgaacccatg cgcttgctcg
    17341 ccatcattga ggcaccgccg gatcgaatcc ggtctatcat tcagaagcat acattgcttc
    17401 aacatgtctt ccataaccaa tggatgaatg tcatagcgtt tgatccagat tccaaaggat
    17461 tttcacgtta tctctcggat tcaacgtggg aacccgtcac gactacaata taattttgcg
    17521 atgggaaggg aagtcttttg gacgtttgcc gtgcttggaa tggcagagcg aatcccgcat
    17581 catctctgat gagagatgtt tgtttcccag ggatttcggt tccacctaca ggatcaagtt
    17641 gtcctaagaa ggctggctaa aagactccga tcgaaccctt cccttgattc gcagttgtgg
    17701 atggaagagg gtgcctggta taaggtcaga ggcttgtggg ttgttgaaaa ggatgaggct
    17761 cctctttgta tttggcgcag accaagtagg gagagtggga gcatccggtt ttccgaaatg
    17821 tcatttgcat catggccgta gggttcccag cattttgttt gttccgatag gctggagaca
    17881 gtacatgcca accaggtttt ttgaatacct cattcgaaaa acatatggtt ggcttttttt
    17941 ttaccgaatc ggattcattg agtttagatt ttggtctgag aatagcgcag gatgaagtct
    18001 tcttatccga aacagtttat cgctaacacc ttggcccttt ttagtgtctg gcttgtgtta
    18061 tcggggaagt acgacaattt tcacctcgta cttggattca tggcatcttg tggagtggcg
    18121 tggttaaaca cgggattccc tcattctccc ttccataatt tcccatgggc tcgtgtggtt
    18181 ctgtacagcc cctggctctt tcttcgtatt gttgagagta gtctgcactt gacgaaactc
    18241 attctcaatc cctcattacc cattagtccc agactcatta cctatcagtc tcatttaaag
    18301 cataagggtg ccattgtcct gcttggaaat tctgtgacct tgacgcctgg caccattacc
    18361 gttgaagcca acggcaatac gcttctggtg catgcgatag atgggacagc agcaaaggac
    18421 ctcacgacag gccggatgga gcgtaaactc gcctgggtct ttcaggaaga tacgaatcac
    18481 tgatgcgtga tttttttctg tttttattaa tcactttgtc ttttctgatt ctcgtgtatc
    18541 tataccgggt gattcagggt cccaccgtgt ttgatcgggt attagggtta gcgggaattt
    18601 cgaccaaagc catcattctg ggtgttgtga tgggaacagt ctatgaccgg gtagaaatgt
    18661 tcgtggatat ctccacgggg tatgcgcttc tcaatttagt tggagcattt gccattgcca
    18721 aatttcttga gcaacgaggg acaccctaaa tggtcgttct tgccatcggg ttaattctag
    18781 taggggtgtt ttttttagtt gtagctgcaa ttggcacact acgccttccc gacgtgtttt
    18841 cgcgatccca tgcggtctct ctgactgatt cccttggtgc catttgcgtc cttgcgggcc
    18901 ttgctttgta ccagggattc gggatcaata tgctgaagat cctggtcgtc ctggtcttgc
    18961 tcatcctgtt aaatcccgtc attgctcatg ccaccattcg agcggccaat cgggcaggcc
    19021 tcaaacctca gacgaaggaa gacccatgat atattcagtg aatattgctt tgctgttact
    19081 cctggtcctg accgccgcca gcgcaacatt tttggtcaag gaattgatga gttcagtgtt
    19141 tattctcggg tcgtatagtt ttttcctatc tctagtttgg gcttggctgg gtgcggtgga
    19201 tgtggcattt gtggaagcgg ttattggggc tggattgggc acggtgttgt tcctcatcac
    19261 cctgtttgat accgctccaa aagatagccg ccttcgtcgt ccccctcctc ctctggcagg
    19321 agtgctgggg ttgcccttgt tgggcctgtt gttgttactc gcggctaatg atcttcctca
    19381 gtttggcgac ccgacctcac cgcccaacgt gcatatttct cctgtgtact tacagaatag
    19441 ctatgcggac acgctcacgc cgaatgtggt cacctcggtg ctgatggact atcgggcatt
    19501 tgatacgttg attgagaccg tggtgatttt tacggctggc attgcctgtg cgttgttatt
    19561 aagggagcca ggctcatgaa acagatccat gacagcatca ttgttcagag catgagtcga
    19621 ttgctcattc ctctggcaca gttatttgcc ttttatgtct tgttcttcgg ccagtacggc
    19681 ccgggaggag gatttgtagc tggcgtcatt tggacgacca gtatgatcct ggcctttttg
    19741 gtgtttgggc tgaagtcccc tcaggggcgt ctggtagaaa aggtgttgca tggcgatggg
    19801 atcggattga ttatttttgt gggagtgggt ggtttatgtc tcatcggagg tggggaattt
    19861 ctcaactatg ccaatttgga aattccgggt ttggatgctc cggcacgtcg gtacatgggg
    19921 attctattag cacaaatcgg ggtggccgtg gacgtggcgg tgaccggaat ctctattgtt
    19981 ctcagcttgg cctatcacga tactgagcag gagtatgatg tttgagttga ttggggggtg
    20041 gctccagcgc cccaactata ttgcctttgt tcttctgttt ctgtggggca tctatattat
    20101 ggtgacccgg tacaatctgg tgaaaaaact catcgggatg tatctcatgc aaacaagtgt
    20161 gatttttttt ctcgtcacga caagtgccaa gcgagacgct acggtgccta ttttgctctc
    20221 cactacggag attattcagc cggaagtcta tgccaatcct ctccctcatg ttctgacatt
    20281 gacggcgatt gtcgtgggcg ttgccaccct gggagtatct ttggccctgg ccgatgccat
    20341 ttataaaaaa tacggcagcc tggacgaaga agaaattctc aaaaggatgg aatgacgcga
    20401 caccttcccg taatcctttt cctcttacct ctcggtttgg ccatttgttt accattaatc
    20461 ggtgtcaggc gtcgggagtg gtgccggcca ttagcgattg gtgtgctggc tgccatggtc
    20521 ccggtctcct tcgccagtct ggcaggagtt cttcaaaacg ggaccattca ttatgacttt
    20581 gggggatggg cggccccggt tgggattgag tgggtggcgg acggattggc cagcatcatg
    20641 actgtggctt taagtctcgt cgggttggta tgtatcacct tcctcagctc ggtattattt
    20701 ccctatctcg gcagccggat tgtccctttt tataccctgg tcctgttatt gatcgccagt
    20761 ctgatcggca tggtgtttgc cggagatctg ttcaatcttt tcgttttttt agaagtatcc
    20821 gccttgtgtg cctatgcctt ggttggattg gctggtggga aagctcttgt cgcaggattc
    20881 cggtatttaa ttctaggcac gttcggagct tccctgtatc tcctgggggt agggtactta
    20941 tatgctgaaa cgggcacgct caatatggcg gatctggcgc agcgggtgcc acccttggtg
    21001 ggctctaagg ccatcgctgt cggagtgtta tttatgttta tcggtctggg gatcaagatg
    21061 gcgttggtac cgctccacgg gtggttgccg gatgcctaca catatgctcc ggaccgcgta
    21121 tcacccatgc tggcttcgct agtgaccaag gtcgccctct atggatggat ccgtatcatg
    21181 ttttgggtgc tcggagccca tgcggtagtc taccggattc ccgttctgtt tttagtggga
    21241 gtgttgggcg ctcttgctgc tttggttggc gcattcctgg ctttgtccca gacagaaatt
    21301 aaacgcatgt ttgcctatgg agggctttcg catatcggat tgattctggt gggtattagt
    21361 cttgggaatc aaaccggttt tgtcggagga gtgttttatc tcttgaatga tgctgtgatg
    21421 caggcgggtt tgtttttctg ggcaggggcg attattcacc tgtatggggt tcgcacaatt
    21481 gaggatcttg ggcgattgca aggaggagcg ggatggatgt cggttgctct ggtggtcata
    21541 gccctgtcga tgatcggaat tcctccaaca gggggatttt ttggaaaatg gtatattatt
    21601 cttggcgccg tggaagcgca gaactaccta gccattgtcg cggtgctgat tgcgactctg
    21661 ttaactcttg cctacttcgc taaattgttt gaacggctgt ttcgggaccg gtccgcccag
    21721 cagcctcttc ccatacgtga gattccgttg gcggtgcgaa ttggtctcgg cgcaacctca
    21781 gtaggggtca ttgtgttggg agtgatgagc gatcacattg tcagctttct cctcaagaat
    21841 gcgcttccgc ctgggcttta ggagaattgg gcatatggtg tatttctgat atggcattcc
    21901 tcatccttat tcccctcctt cctctgatgg ccgttctgat tctcgtgctg gggaaaccct
    21961 ggctaggcga gcaaggtcat aaaatcggga tcccggcgat ggccctctcc ttcgcttgtt
    22021 ccatcatcgc gtttgcccaa gtggcaaccg acggggcctt ttccatccct ctgtatcgcc
    22081 tgatccattc tggcgacctg gttgtcgatt tggggttgta tgtcgaccaa ctcaccgtgt
    22141 tgcttctgct cttggtgacc ggtgtgagca gcgtggtgca tgtgtacgcg tcccgctata
    22201 tgatcggcga tccccggtat aaccgattct ttgccgtcat cgcgttgttt acgttttcga
    22261 tgatcatgct ggtcatgagc aataacctgc tcatgatgtt tgtgttctgg gaaatcatgg
    22321 gtatctgttc ctatttgttg atttctcatt ggggtgagcg gcagccatct gctcaggcgg
    22381 caaccaaggc ctttctcgtg aattcggttg cggatgtggg gtttggtttc ggggtcattc
    22441 tgacctattc cacttttgga acattggata tccagacgat tttagcggaa gccccatcca
    22501 tacagggaaa caccatcaac ctgttcggct ggatcgggtt ggacttcccg attcatatca
    22561 tcacgctcat tacactcttt ttattcctgg gtgccatggg aaaatccgca caaatgccat
    22621 ttcacgtctg gctacccttt gccatggaag cgcctacgcc agtttcggct ttgattcacg
    22681 ccgcgaccat ggtgaatgcg gggcccttct tgttggttcg catgagtccc ctcgtgatac
    22741 tttcgcctga tgccatgact gtcattgcgg taattggggg cctcacggcc atttttgcga
    22801 ctctggtctc cctgactcag gtggacatta aacgcatgct ggcgttttcc acgattggtc
    22861 aaatcggttt tatgatcatg acctgtggag tcggggcttt tgtgttatct attttccata
    22921 tgctggccca tggctgtttg aagggatttt tatttttatc gacgggaaat gcgcttcgtg
    22981 tggttggttc tcatggacat gaaccaactt cattttcccc acatgtgtcc tcgaaccatt
    23041 ccttgtggcc aatcgtatca ggggcattgg tgttagcctg tttccccccc ttccttattt
    23101 ttttcggacc atatgagcaa ttgtggatga ttcaaccggg gtcctcaggc caatggacct
    23161 tttggatact ggggcttttg acggtatttt tttccgccat gtatgtcatg cgcggcgctc
    23221 tgttgttgtt taatcaacct ctatccaccg cacgtgaaga tggaatggga agccaggcca
    23281 tgaagcctcg tttcttttcc accattcatc ttctgggaat cttggcgatc attggaattg
    23341 tcgggagcct attactgatc ttatggagtt ggtttgccga atttctatcc ccggtcgtgg
    23401 gacatcggat gccgtatata ccagaaaaat cttcgagtgg gcctggcttg caaggtattc
    23461 tagcttttgc cgttgctgta tgcggtatcg tggttgggtt cttttccatg acaaaatttt
    23521 ccggatcctg gtttcaacaa tcagaaatgg gcaagcgctg gtatgtccat ttcctgaata
    23581 aattgtattt tgacgaaatg tatgaagcct ttgttgtggc gcccactctc tggtttgccc
    23641 gatggctatg gcgggttgtc gaccggaaag tgtttgacgc cctgatcatg ggtatcgcga
    23701 atgggtcggt gttggcggcg aagtggatgt ggcgggtggt ggaccttcgc ggaattgacg
    23761 ggctggtggt gggtttaggt cgaaacagcg taggaattgc cgggtggctc tggcgggtgg
    23821 tggatcttcg aggtgtcgat cgggtcgtgg taggtatcgg tcaacgtagc cttggtatcg
    23881 ccgactggct atggaaaagt atcgagatca aagttctcga caagaatgtg aatcgtgccg
    23941 ggaatcaagc agagactaca gggaatttga tgcgggaatt ggaaccccgc acattacaac
    24001 accatttact ggtcatgatt ttctggctca ttttaggcat tgggctatta ttttggcttg
    24061 ttgtgtgatt cacagggggt ttttcagcaa tgtggggaag acgcgttcgg catgaacaaa
    24121 caaggactct gggtttaaaa aaagcgatag gacgtctttc atgttttggt ggtgtcatga
    24181 tcggcatttc atgaagggga gactatgaat acaatcttta ttgaacatct tttgagtgtc
    24241 atgattgccg tcccgtttgt gggcattgcg attattgcct gtattcagga tcaggaatgg
    24301 attcggcgcg tggctctcgg gtgtaccatg ggggatggtg ctctctcatt ggtgctatta
    24361 aaagagttcg atttttccat tcaaggcatg caattcgtgg agcggatggc atggatgccg
    24421 acctttaata ttcaatatgc tgtcggtgtg gatgggatta gtcttctcct ggttttactc
    24481 acgactttgc tctgcccgtt ttgcgtgctc tgttcctggc aatcgatcac tacccgtgta
    24541 cgctccttta tggcgatgat tctgttggtc gaaggggcca tgatcgtcgt ctttaccgct
    24601 ctggatctgt ttttgttctt catgttgtgg gaagtcacca tgattcccat gtatttcatg
    24661 atcattctat ggggtggccc ccagcgcata gccgccggca tcaaattcgt cttgtacagt
    24721 ttgtcgggaa gcctgatttt actcttggcg atactcgggt tgtatgtgga aggagggcgg
    24781 acattcgata ttttagcgtt ggcggagaag acctattccc ccgacgccca gttttggata
    24841 tttttggcgt tcttcctggc ctttgccatt aaattaccca tgctgccttt tcacacatgg
    24901 ctaccggatg cccattccga agctcccacc gccggaagcg tcctgctggc tggggtgctc
    24961 ttgaaaatgg gtggatatgg ctttttacga ttttgtctcc ctatgtttcc tgaagcctca
    25021 gtgacctttg ccccgtttat cctgtggtta tccgtggcgg ctattgtgta tggggggtat
    25081 atggccttgg cccaatcgga cctgaaaaag ctggtggcgt attcgtccat ttcgcatatg
    25141 ggattcgtga cattgggaat tttcgtgttc aataaccatg gtattcaagg agccatcttg
    25201 cagatggtga accatggaat aaccacagga gccctctttt tggctgtcgg tcaattgtat
    25261 gatcggaccc atagccgttc gattcaggat tacggaggat tgcaaaaatc gatgcctaga
    25321 tttgtggcac ttttttgcct gttttcagtg gcatctttcg gactgcctgg cacatgtaat
    25381 tttattggtg aatttttggt cttggtcgga acgtcttttg aaagctatgt catggtgttg
    25441 ttgtccatgg ggggcattgt cctcgcggcg gcgtatatgc tctggatgct ccagcgggtg
    25501 gtgttgggac aaccaaaaaa ttccaccatc gcacaactac cggatctcag tatgcgtgag
    25561 ctggccacgg tgatccctct tgcggtatta atcttgggga tcggtttgta cccgtcacca
    25621 cttatggaaa tgatggattc gagcgtcacg cacttggctc agcatatggc acagtatcag
    25681 gtggggatga tccatgaggt ggcccttcgg taaaagactt aactggcagc agtcgcgaca
    25741 aagattatcc ctccgaaaag cccctgttgg tagatttctc ggttagtgac cccgagggat
    25801 gaccatcaga cttcttttat ccccgaatcc cctcttgcca atattttccc ccttcatctt
    25861 tcctcctaaa tcccctggga attgacgcag actcagttgt ggaccttgat gttgacaggc
    25921 cattctgacg tttttgccaa aatttcacag tgttcttgcc gttttctcaa gtggcctgtc
    25981 aaaaatgccg agaataatct tctctgctct cttggggggt ggaggcacct tccagtcact
    26041 agaggaagga tgagtgtcgt acccagaaaa gtagatcgaa tcaatttaga ccaatgcgta
    26101 ccaatcagtc ttgaatcctc aggtttcatt gattgcagag aaattgaggt gaggaggggc
    26161 ggtaaacgtg gaggagcaga ccatgaaact gatcaatgtt ttacttgtgg cagtggcgtg
    26221 tacgggtgtg gtcgggtgtg tgagtcagca atcacatgaa cgtgctcttc aagaatacga
    26281 aaagagccaa gagtccaccg ccgtggaact tgaacggttg cagcaggctg ttgaagagaa
    26341 gcaaactgaa gtcatgcagc ttcaagaaac caagatggcc ctcgagcata ccgttgaggt
    26401 gcaagatcaa ataaaagaag aacaactagc cgcggttcaa caagaagtcg aagcgctgta
    26461 tacccaaatc cggaaactcg gaaacacgga aggagtcggt tctctccccg atcatctttt
    26521 cgggggagag ccggtggatt tccaagattt gagggcagca ctttcaagga tcaggtcggg
    26581 tttggatggg caaattggcc aggtgtctca ccttcgtgaa gagaatagtc tgttggagaa
    26641 ggagctttcg gctttggaag ctaggctcaa ggaagtggaa cggttaaaac atgagttgga
    26701 attggtcaaa aaagctcgag aggctcagga agcgcaactg gcgaaggtca agtatgaagt
    26761 gctcacagtc ggagaagaga tcgatcgcat taccaaagcg cttgaggaca aatttggcaa
    26821 aagtcttgtg gtgactcaac accatgatcg attggtgttg accatgctgg gtcaagtcct
    26881 ctttgaatcc ggtgaggcgg atttgactcc tctggggctt acaatcatga aacaggtggg
    26941 ccaggtgtta gcgaccttgc cagggaaaaa catccaagtt gaaggccaca cggacaacca
    27001 ccgcatttat ggacagctgc aaaaacaata tcccacgaat tgggaacttt caaccgcccg
    27061 ggctaccacg gtattgcggt atctgattga gcaaaccggc atggatccca accaattctc
    27121 agcgacgggc tatgcggata ggcgaccggt gatgaccaat gagaccgagg aggggagagc
    27181 gcaaaatcgt cgtgtggaaa ttgttctata cccggaacgg gtcgttcagg atattgaaac
    27241 tgttgccacc ctggcccggt aatacaagcc gggaaccaca ataatcgata tggcttcaga
    27301 tgtggtgctt ctgagctttc acatcccttt tctcctccct tcagtcgttg gtcagccttg
    27361 cagcagaggt gcagtttctt ctccccggcc actatcggat ttttccaaca attaatgcgc
    27421 tgccggtact gtgggattgc cggagctact cgggctatga tgttggagga gtccggtgtc
    27481 gtatagatat gctgttgccg gatctaatac catatacacg gcaaaaagac cgacactcgc
    27541 catggtcaac gtatagggaa gcgccatcca gaccattttc ccatatgata aacggataac
    27601 cggagccagt gaagaggtga gtaaaaataa aaatgcggcc tgtccgtttg gcgtcgccac
    27661 actgggaata ttcgtgccgg tattgatggc aacggccaaa aggtcaaatt ggtcacgggt
    27721 aatggtgccc gcctggtagg cgcggagcac ttcctggaca taaaccgtcg caacaaagac
    27781 attgtcgctg atggccgaaa gaatgccatt ggccagataa aacatgccga tttgatgcga
    27841 tccctccaag cttaatccat agccgataat gcccgaaaac aggttctggt cctggatcac
    27901 ggccacaatg gcgaaaaaaa ccacgaggag cgccgtgaag ggaagcgctt ccttgaacgc
    27961 ttctccaagt tgatgctcat cgataatccc gttaaaggcg gtaatgaaca cgatcaccgt
    28021 gagtccgatg attccgacct ccgccaggtg aaaggctaag gcaaaaatca accaaatgcc
    28081 cgcaatcgct tgaataataa gcttcgccgt gtccgcggaa tctcgtcgtt tctgttgctc
    28141 ggtttcgaat tccaccaaaa tgaggcgaac gggatccggg agttgaaagc cataatcaaa
    28201 ccatcgtaat ttttcaacca tgatgcaggt aaacaatccc gtggccagga ccggaagcgt
    28261 cacgggggcc atgcgcaaaa agaactccag aaattcccag cccgctttgt ccgcgattaa
    28321 taaattttgg ggttccccta caagagtaca gactccacct aatgcggtac caaccgctcc
    28381 gtgcattaat aaattgcgaa ggaagcctcg gaaattgtct aaattttccc tgtgaagaga
    28441 gtcgatctca tgatcgcttg tggaatcatg agaggtctgc gacgatttgc cggaggccac
    28501 ccggtggtaa atgttataga accccaacgc gacggcaatg atgactgctg tgaccgtcaa
    28561 ggcatcgagg aatgccgaaa gaaaggcacc catgatggaa aacaagagtc ccaacaaaac
    28621 ttttgagcgg acgcccaata aaattttggt aaacagaaac aggagtaaat ctttcatgaa
    28681 gtagatcccg gccaccatga aaatcaatag caggatcact tgaaaattat tgagtgcttc
    28741 atgatagacc gttgccggag tggtcatccc caataagaca gcttcaagtg cgagcaagcc
    28801 accgggttgt agcggataac ttttgagagc cagggctaag gtaaagataa attccagtat
    28861 gagcacccac ccggtgtaaa aggaaccaat gctcatcaat aagatgggat tgagaattaa
    28921 aaatcccagg atagtcaact tgtaccaggt tggagtgtgg ttcaagaaat tctgtatgag
    28981 cgcctgagga actgtcgttt tcatttcagt taggtgacct ttcggaccgg tggtcccgga
    29041 tggagtctga ctcattaatc caatatcact atggtcaatt cgttgggtag tgatctcttt
    29101 cgtatccagg cctttaatag ggcggatgga gtgaggagtt cagcatatac aatgaaggct
    29161 ttttcagaaa cagggaaggc gacattgtta aaaaacctac cttaggaggg ttccggattt
    29221 ttagacagaa cttccgtggg aggggtatcg aacggatggg tggatccgcc gatatgagct
    29281 gcgtggcgta atgcatccaa ccgaagaggg tatcgatgct caggtgggac ctgatccagc
    29341 accccgagtc ctttgaggac tcgagccact cctggacgca tacccacgaa aaaaacatgc
    29401 tgctgatgag ttttcaccat caggagcata tcttcgatgg ctaatgcagc ggttccgtca
    29461 atagacggca ccgatgtgag atcaagaacc acgctggaaa aagaattgaa ccctttcaca
    29521 gattctaacc gacgaaccat gtccttagcc gatccgaaac tcatcggccc attcacatga
    29581 atcatgataa tgcgctcgca gtttcgatcg aggatgcgtt gctcctcttt taacagtggt
    29641 gtttcgttcg aggcaccagt gatcacactg agattttcaa gttccaggtc ggccatgcgc
    29701 ttcacgaaca ttacactggc caagaccacg ccaattccca cagccgtgat gaggtctact
    29761 aaaacagtaa ccaacaagac cacgatcatc accagaactt cggttcgtgg agcttgcacc
    29821 atatgtttca gaaatcgcca atcgataata tcgaatccga ctttgataag caggccggcg
    29881 aggactgcaa gtggaatttt ttcggccaga ggacccaagc caagcaacgt acctaggagg
    29941 acgaccgcca taaacatgcc agagataggc gtacggcctc cagtgcgaat gttcgccaca
    30001 gatctcatgg tggcgccggc tccaggaagg cctccgaaaa atcccgcagc catattccca
    30061 atgccttggc cgatcaattc ccgattggag tcatgttggg tccgcgtcat gttgtcacag
    30121 acgagagaag tcagcaggct atcaattgtt cccaataatg ccaggacaag ggcttcctca
    30181 accatgagta agagtgtact ggtatgcatg gttggccaaa ttagcgtggg aaatcctgaa
    30241 ggaatgtctc cgatcaccgg aagggtcggg aagaacatct caccgacagt cgttccaacg
    30301 atgagagcca gcaacaccgg aggaagacgt ttgccgatag aggcaggagt gagatacaca
    30361 atggctagtg tgagagaacc caccaacatg gcgggaagag aaaaagatgc gaggtactcg
    30421 ggcatcgcca tgaggttggc cacgactttg gacttcgctt ccagtccgag aaagggtgca
    30481 acctgaagaa tgagaataat tcctccgatg ccgctcatga atcctgaaat aacaggataa
    30541 gggaccaagg tgatataacg accaaacccc atcaatccaa ataagacttg aaatccacct
    30601 ccgagaatga cggccgtaaa tgccactgcc ggttcatcgg caaagttcaa gatgatgccg
    30661 gccattacca cggtcatcgg accggtcggt cctgaggcct gtgcaggcgt cccgccgaag
    30721 agcgctgcga aaaaccccac aaagatagcc ccatagaggc cggctatggc accgagtccg
    30781 gatgtcactc caaaggccag ggctaggggt agagccacga ccgctgccac taatccacca
    30841 tacaggtctc cccgtatgtt attgaaatga aggccgtgaa cccaggaaag tattggaaag
    30901 gtagctgcca ttttttagga gggaggccgt agggttcgat gatgggtcac aaatgctacc
    30961 aggaaaagaa atgttacttg tatgtcgttg ggtatcaagg aatcaggccg aaaagtgatt
    31021 cttgtgaacc tcgattatcc attgagacct ggcttggtgg tccgttggat gaattggcca
    31081 atttgaagac caatggatgc aaacatggcc aacagttctt tgtcgggctt ttggatatcc
    31141 cggtgaaaaa attcgatgac tccaagaatt tcgtttcccg atttaatggg aaaggcgaat
    31201 ccggaatgga gaccttcatg tgcggcagcg tcagcgcgcg gaaaattcgg gtcggacact
    31261 acatcggtga cccaggatgg ttcgccgcct ttccaaaccc ggccgggtaa tcccacccct
    31321 cgtgcaagcg tgattgcatg ggatgcctgc cgaaaggcat ccagatttaa gtgaggggaa
    31381 tgccagacat tgaggcaacg taagacctgt tgtgaggaat cgagtatcca aatagctccg
    31441 agatcccatc ctaaattttc accaatggct tccagaataa gggaagcagc ctgagtcatt
    31501 ccagccgatt cggcgagaac attggttaag ccgtgatggg tggccagtcg acgttcttta
    31561 tgctcccgtt cgataccctg gccaatttga aatccgatat tcgcaagtct ttgaagcaac
    31621 atatcatctg cttcctcagg gaatggcgag aaaaattcga gaaccgctct cgtattccca
    31681 gctaggcaaa tgggaatggc gaatccggcg tgaagtccca ctgccagcgc tgcctggttt
    31741 ctagggtctt tgggatagtc ggcaaggtcc ttgatccaca gggggcttcg attgtcccac
    31801 acctgcccag ccaatcctac agctggcgtg agcgcccaac cggagtttga tgatgacatc
    31861 gcttgggctt gaggagagtc ctgtggccaa atgacggcgg ctcccagagc ctgccgttga
    31921 tagtccattc gccagaggat gcccaccctc caatccagcg tgtggcaaat ggctttgaga
    31981 atttgaggat atgtctctga aatgctggac gcggtcgtca agatgtctgc gatggcatcg
    32041 tgcaaggcca agcgtcgctc cgcacgctta cggtcagtga tatcaatgcc aaatccaatg
    32101 aggccaggtt ggtcgccttc caatttgcac agacaattta aaaaccacca gggcttcccc
    32161 tgcaaaatgc cattgctctc ataaaatacc gaattggcaa gagttccttc cttgatttcg
    32221 ctttttatcc gatcaggtgg ttctaatgga attcgtccaa tggcggtaag ttcctttaat
    32281 ccgaaacgag tcaggccgcg accgaaggaa tcatgcatga ccccttcctg atcgattcga
    32341 aatgccacgg ccggcatatt tgataaaatg cctgttagca tggaactttg atcgcgagtt
    32401 gccgcatcag cctctcttac ctgcatggct aatagagcgg tggaccaaat ggcaaacaag
    32461 gctatggagc gattgatcat gccaatccaa ggttctgtcc cggctgggga taaaccgtaa
    32521 cccacgattg ttaagaccgt acacaagccg gcaagacgca aagagtcatt tttttctggt
    32581 gaccgaaggc caattaatac cacggcaaca taggggacac cttccgcaat tccagtagga
    32641 aggaaaaagt ccacgacgaa aactacactg gccaccatga gggcaagcga taagatttgt
    32701 ccccgattat tttgaggacg ttcccccgag ctttgaagag tcagcggact tggcatagag
    32761 taatggtagt attcatgaaa atacggaaaa ttagagaaat tatccccagc attcagtctc
    32821 aatacctcga ccctacgata cgaaaaggac gccacagagt gcaagaggaa tcagcgggaa
    32881 ttgattgccc gggccgtccg tggggagagc atcctattga ttggaggcat tctctgatct
    32941 gcagaggatc tgttcgggaa taggcaaagt ctagaagtgg atcccaaggc tcgacggctt
    33001 ttttcctctt atgcgaagag gacaaactga ggtaggataa tttttacgcg tatgagttcc
    33061 acatctcctc aggtccatat ctatcacgtt gccgggttgg atgaccgtga tcgcgggttt
    33121 agtcgacaaa tccaggttct tccccgtgaa ggaggatttc aggccagact ccgctatgaa
    33181 tcgcttcaga tagatgtcga ccccgtgcct accgaagacc aggtcctgct aggtctcatt
    33241 cacctgttgc atgatagagg ctatcggcaa ttacgaaccc aacgtatctt tattggtgaa
    33301 cagtatcttg ggaatcagga gatgtgggtg gaatatcccg atcccgaagc tcctcctgaa
    33361 acgaggaaca cctgggtggc ctggcttcgc cgaatccttg gtcgttcttc ttcaccatcg
    33421 tagggctcct tgaatcacat aatatcgcaa gatcaggttt tgtgagtgag gacgggatcg
    33481 cgagatatac cttggagtga ttatcaggat ccggccagag agttcagggc tccgactcgg
    33541 gattctgcgg gagaagctga gggaaagtag ctgataattc gacgaggatt gaggcattcc
    33601 ctaattctgg gagaaaggtc aaccggtcga atggcactga caatagagag ggattcatcc
    33661 tcttcggaca gattcagaat aatcggttca tttgaagagt ttgattcatc atagacaagg
    33721 atgacgggcc ttagccgatt cgcgagattc acgctggtga caatcccaat cttctgatta
    33781 ctcaagttga ccagtgagcc tggagggtag acacccagtt ggctgattaa tgagacgacc
    33841 gcgtcttgcc atagtgtgtg tcgacatttc acgtataaat aggagagggc ctctgaggga
    33901 acgagggatt tggttggatc cgggtgatga catagttcat cgtattcatc caccaccatg
    33961 acaattttgg cgaacatgct gatttgcggg tcttttttcc ctgctgggaa tccagaccca
    34021 ttgaggcgtt catggtgccg gcaaatcacg tcaattgctt cataggggaa attaggcaat
    34081 ttctcgacca tctctacccc gaatttcgga tgagtactca aaaagttttt ccgttcgaaa
    34141 gcagacatcg acccttttcg taataattgc tctgcagaaa acctgagttc gcctatatca
    34201 tgaaacagag ccccaagggc cagtttttca gcgtcttcac gcgaaaatcc taagtcctgc
    34261 ccgaccatga gagacaatga acacacgttg agggcatgaa gaaaaaattc ctcgttcggt
    34321 tcgttggaac cgatcagatt cagtagcgct cttgagttgt cagctatatc aagaatctca
    34381 taaagctccc ccacaatttt ctctgctgtc cggagtccgc gaggtcggcc ggagatcacg
    34441 tcctgcatga cctgtttggc ttcttgcacc acctcctgga attgtccacc aactgcttgc
    34501 aaatgttcct ggtaaacttg gaaagcttca tgttgtgcaa tcttccgttg aataggattt
    34561 tcaggaaccg catcttcttc tgaggaatcg gtagtgaggt aggcctcaga ttcgtcagag
    34621 gattttcccc ccggtttctt tatgttattg acctgcaccc ggtctggttt ttccgtgcaa
    34681 ttggaatgag ccatttccgg gtctgagcgg tccggatcga aatacaattt gacatttgtg
    34741 agttcacgaa tggtttcaag atctttcgtc aattcgattt taaatgaatt ggttgggaat
    34801 ggatgactga accaggatcc ctcgatcttg atgtacaatc ccagtcgaag agcggttcta
    34861 tgaagtggaa cgaaggccat ggtagctcct tatttcagtc atttgtcatg actgggctgg
    34921 tttcacggtt gatccccggt tttgtccgtg ggcacaagaa atcgcatgtc cgggttgttt
    34981 gcgtcgttgt aggccgtttt cctattgaaa ttgtggccac tgatatttgt cggaaggaat
    35041 gagccaggaa ttaaggggaa gttcggaaaa tgtgtggaaa agagaggaaa ggggagtgca
    35101 atcgaagatg ttgggtgagt cagtatgggt gtcccctcta tgaggcggta gtgccgatca
    35161 atcactctgg gctggtagag aactctgagc gatgccctga ttgctttatt cctgttcccg
    35221 accacgttac aacggaatcc cattcgctcc ggtataaaaa cctcaaaatg taaagagagt
    35281 ctggaaaaaa ctcattgcca gtcccgtgga tgaaaacgtc atcacgcgct tcacaatgat
    35341 gaggggtatt atcgttctcc ccgaagactt aaaggtttgt ccggggtgga gatctgcgtt
    35401 ttggtcatgg acgttaagct ggcaaataat ttttttctga taggaaagaa ctccggctgg
    35461 ccttctgtta attgtaagaa tctaccaaaa tatttattcg ctaaccgatt attccccatt
    35521 cgctcataga catagcccat gttcaaatgg atatggggca aatccggttg gagtgcttcg
    35581 gcttgccgaa gttcatgtac cgcaagttgg aaacttccct gttgttgtgc caccagagct
    35641 cgttgaatcc ataattgtgg gactttgtcg tttcgtgcga ggccgctcag aaaatactga
    35701 tcggcctgat cgagctgacc tgttcccaaa tacgccgttc ccatccaaaa ccatggttcc
    35761 caagtgacgg ggggatcatg gaagagtggg gagagtatcg cctgtgcctc ttcgtatttc
    35821 ccggtttgaa tgagttcttg gacgtgatga agcctattgg ctggagatcg ctgagccggg
    35881 tctgtgacct gaccattctc ctgagtcccc tcatttttca gaggaagggc tatcgtatta
    35941 ctagggatag aaggctcgtc gttaacagga gctgtggtgt ttgcaaactc acctaggatg
    36001 attttttctt cttctaaatc gacttccaac ggaatggcaa tatctgtctc attgacggat
    36061 gtggattcgg ccaggtccga atctgattgt tctgtctggg caacgattgg gataggagag
    36121 ctggtcccgg ttttcttcgg atcagtgctc tggtgtttcg gtgatgggtc tggtgtttgt
    36181 ggtggcatcg aaagggacgt gggcacggtt ttctgctgtg ggtcattgaa tgtgggggtg
    36241 actactggtg tttcaggtgt ggaggaggcc ggaatttctc ctacgggtgg tgtgaggaga
    36301 gcatgtttga cattcaatgg agagggcagt tgcgatgaaa tactctcttg agatgtgggc
    36361 gattccccat ccggttcctg ggtagctgat attggaaggc tctcaactgg gtcagcagtt
    36421 ggtatctgcg aggtttcaaa agaaactgaa tcagcaggcg gagtctccgc tagtggagag
    36481 aagttcgaaa gagccacatg ctgactggga aaaggggagg cctccgttgg cataccaaag
    36541 tccaaatgca agccgatcca gtaggcaccc agacctagac tgctcaatcc gattgccatg
    36601 ccgatcccga tcaaccaaaa ctttaatgga gagggccggc ggtgccaacc cggctctctt
    36661 ctcactctag ctggaaggac aaggggggcg gcctgttggg tcgtctcagt cccttccctt
    36721 ttggtctggg tctgaagtcg ctggagggtg tctgcaatga tactcatggt ccgttggttt
    36781 tccgtgatcc tctcactaag ggagaaattc cttggttgcg cgccagatca tcatgggagt
    36841 caccgtatgt cggccttgag cataagcggc taacagggcc cggtcgcaca gttgattgat
    36901 gcggcgtggg actccacgac tccaagcata aatgagccag aggactcccg caccaagatg
    36961 aaatcggtgt ctggaatcgg cgatatgcaa tcgatgtcgg atgtatgcac gagtctcctg
    37021 ccaggtaaat gactccaggc gatagcgaac gctaatgcgc tgccgcaatg gtcgaaaggc
    37081 tcgagtcgcc aacttttgtt ccagctccgg ttgtccaacc agcaggagac acatcaattt
    37141 atctttttcg gtatccagat tggagagaag ccgaagtcct tccaggactt ttccactgag
    37201 ccggtgcgct tcgtccacca acacgacgac ccgttctcct tccacggcca aacggagcaa
    37261 cagcttaggc agttcccgtt gtaaggcccc aagggactga tcctcaggca gtgttccggt
    37321 caaatcttca taaatggata caagtaaatc agaataggac tgatccggat tcatgaggta
    37381 tgcaaaccgg accccggtcg gcggatggcg aagcaggtgc cggcacaaca gggttttgcc
    37441 gagaccaact tctccagtca gcatggtgag tccaccactg gcgataccaa atctgaggtg
    37501 gtccagcgct tccagatggt ggctgccggg gaaaaagtaa tcggtatccg gtgtcaggcg
    37561 aaatgggggt tcagaaaacc ccaattcctg atagcacatg ctgagatcta acgtcgtcat
    37621 tatgaggctc gaataatttt aggagagaga aagatgacca gctcactttt ctttttagct
    37681 gtgtacgttc ccttaaataa gcgaccaaga tagggaatgt cacctaagag aggaaccttg
    37741 cgctcggttt ctgagctttc ttcttggatg agtccaccga tgatgaccat ttctccatct
    37801 ttcaatcgaa ccaaaccgga ggattgcttg acatccaaaa ccggagcagt ggctcctccg
    37861 ccatcccccc ttttagcacc tggcgattcc ctaatttcac ggagacggga aagaatagga
    37921 gtcacatcca gcataatcca tccatcttcg gagatttggg gtgtaacgga aaggaccaag
    37981 cctaccgtca cggatcgcac ctgctcagtg acaatattgc cactcccggc agttccttga
    38041 gcaacggttg aggaaaaaaa aggttcatcg gtggcgactt taattaaggc cggttggtta
    38101 ttcatggtaa ccacgcgagg ttgagagatc acacgaatgt cgccctgttc ttgcagggct
    38161 tcgagaacac catcaaaact cccatcctcg aaggagatag tggtcgtggc agctttcgcc
    38221 atgaatcctc cgaagggcgc agtaattata tttgcgagag caatggcccc atttgtgcca
    38281 tcaaaattaa ttctgctcca atcaatgccc agactgtact gatcatccag ggcgacttca
    38341 taaatccgca cctcgatctc tacctgtcgg tatagggcct ggtgaacgct tttaaggaaa
    38401 tcgtcaattt catcgacccg tttataccga tcggatatct gaatagtgcc tgaaagacga
    38461 ttgaccacga gtcgtccttc ttcggacatt agagttttaa tttgcatttc caattcttcc
    38521 cagaatttta tttcgtcctc ctggttcacc gtgatttgtc cggtgtcttg tgatgcgccg
    38581 cctccgctcc ccccgctgga accggagctg agctgcgcgc ggttttgtcc tgttccactc
    38641 cgaacgagac ggatgtaatc gacgttcaat gttttcgttt tgaattgcct gacctggatc
    38701 aggttctggt ggtgttccca gtaatagcca tgggcttcca aaatggctga catcgcctgt
    38761 tccaaaggca gatgatgaaa atccaccgta atggtgccgg tgatgtctgg cccggtcaca
    38821 atatttaaat ggttgctccg agcaaacaac gccaacgcgt cgatgacagg aagatcttgt
    38881 gcgcgaaacg aatacaaggg gcccaaagaa ggggcaggtg ggggtgtcgg aggcttgggg
    38941 agatcgctgg tgagcaacat acgatcctcc agggaaaatg tggcccgaag gttgttcacg
    39001 gatgatgccg ccaaatctga actcagggaa aggctctgtg aagtctgtgt ggtgttggca
    39061 tctgagattg accggtctgc atctggattg gcgatggtgc atccccacag gctgagcatg
    39121 cccaaaagag ccagactttg aaaaactcgt acgccatagg tgatggatgc tccgttggac
    39181 ggtcgattgg aaataatgat cgtctgagga ttcactatgg tgttgtgtcg catgtcgtct
    39241 ttcatagagt gatgaggaaa attatgacgt ggcttttttg ggaaatgtta accattggtt
    39301 tttgccacga tgtttgagcc acacgccttt gggcagaatg gacaggactt gatacccgtt
    39361 aatcatttct ccttcataca ccacggtccg gttgatcacc gcactccgtt gttcaccttc
    39421 gacggcaatg gctttaagag tgaacgtatt cgtcacctgc ttctcctctg tttggtccgc
    39481 tcccatcgaa ggtggatccg attgagcgtg agaagcatcc acgaaggggg caaaaggatc
    39541 acgactggag tattcgatcc attgaccagc ttcatcgtca tggggttgcc ccgtaggggt
    39601 aaggggaggg agtgtcgggg cagaaagcag tcgattcagt cccacggaga ggatgcccgg
    39661 tgcatcggat gccgtgtcca cgaaattggt atagaccgcg aatcctgcaa gaaggcacag
    39721 accaatcgcg acaaacggat tttttaaagc tcgacggaat cgttggtttt catccatacc
    39781 gacaatccaa tggttaaatg agtggcttgt tgaccatcac ccatgaccgt cacattctga
    39841 atgtcgactc ttggtccgga tcgtgtcagg gcttttagga actgaagata ggaatgatac
    39901 gcatgggggg ttccctgcga gttgacttga atttccaacg gaaccaatgt cactccctgg
    39961 attggagtgg tggttcggtc tgtgctcaga atccgatatt gaatgaggag attccgctgt
    40021 ttgccgtcga attgaagcgt ttgcgcccat tgcgccaaat gggtgaagtt gtggagtaag
    40081 cgttgctcag ccagttgaat atgggtgtca agcgcatccg gacgagcctg aaggtattga
    40141 gagcgaagct ccgacacctg ttgttccagg ccgatcgtat cttgagttaa cgattgacgc
    40201 ttttgaatgg tgaaaagttt atccagactc acccagccca caatggataa cgttccaaac
    40261 agtagcacca cgagtgccgt gcttttgagc tgttggggct tgggccactc aaagcgggga
    40321 agccacttca tgatatggtt cccttcaaag tgaatcgata gagtgggcgt tcattggttg
    40381 gggtggcggt ttgtgtcaag agttgatctc tccaatcctt atgaagggac acatgatatg
    40441 gtccatccgc cagcttttgc acgaattgat ccaccacaag caatgtggca gcaagattgg
    40501 tagaagtgct gccgtccaac tccacgttcc atccgtccgg ggttcgctcc acaaaggctt
    40561 tgcgcaaaat gacctgggtg ggaataatgg atcccaaata gctgagaaac gggccttcta
    40621 attccggcgt ggttgtttcg ctgacggaat gagcccattg ccgtttcgtg tctaacgaca
    40681 ggagacgact ttcccattgc gcgtaatcct gctggaggga ggtgacctgg tcggtcatgg
    40741 tctgcatcgc tgtgcgatgt ctggctaaat acccttcgat gacgctggtt gttccgactc
    40801 caaaaagaat aaaaaccgca agcatggaga cgaaagcctt ggtgagggtc tttcgtagcg
    40861 gggcttggag aacctgcgtg ggcgtgaaat tgctggacag attgaccgga agcatcgcgc
    40921 cgacccacag ccagtaattc cagtcgggtt taatggggaa tggcacaagg ggagtgctca
    40981 aatgtggctg aatttcacca gccgtcaatc gttcttcatc gtcccaaaac cagatgcagg
    41041 gaaattttaa attggtttgt tgtgtgataa acattatcgt tcgattggcc tcggttccga
    41101 cccgctcccc aagtggaacc cagtcttggg ccgggaacag atgtcgagtc cagatcaggg
    41161 ttccatcatc atttcctgcg acgatcatga ttttgccttc gatcatggtt atcagcatcg
    41221 aaccttttcc cggttcgacg ggcaaggcgc tgagttgact ctcagaaaga gtggaaagag
    41281 gagccaactg acgaagagac aaattcaact cttggcagat gtgcacgatc ttgtcgatat
    41341 agtcctgtgg ccatatatca aggtgaatat gtaatttccc tcgcgcttcc aggccaaccc
    41401 gatatcgcca agccgccggt ccttcccaag gtttttcctg ttggaccttt cgttccagaa
    41461 agggaaggag gtcggtgggg agcatcggag ggatttgaat ggtccgcgtg gcaagccgcg
    41521 gatggtccac gagcatggcg aggtgcgtac ctggaaattg ggtgtgatgg atcgcttctt
    41581 ccaatgcgtc gcgaagagtg tgtagggaca cgagccgtcc cggttgttcc caactctgtc
    41641 caatcgtgtt tttcacgatc gataaggctt tcaatcgccc attggaaagg ctaacgctta
    41701 aaatcgtctg ttttttcctc agcagagtct ttcggaggtt tggtgggatt tccagaatgg
    41761 gtgtctgaac ctctgaatac gatgtgggat gttgaagagt gtcgatcatg gtcttagggg
    41821 ccataacacc cgctactgtt gagtcgccat tgggatcctg ccgggcagtc ggggtcgaat
    41881 tgaaatccgg cttcgccggt cagggtgaat tgcgtttttt gaccagtaaa tgaattcgtt
    41941 tcatccaaac tgattggggt tccagtgaga tgatagcgaa tatgcggcac aagcgtgccg
    42001 acacctgcta cggtttggct cccatcgatt gcgtagctgc ctccggcgcc attggcagaa
    42061 agactgagga gatgaaataa atggctaacg tgtccacggt gaattttaag atcgggcgta
    42121 ccggtttcat cggtgctcca ccaggtttca aaatgtgcat cggtcgtaat ggaggggttc
    42181 acattttgtg cgagatgggt gatgaccata aaccgcgcat ccgccaaagc cgtcgttggg
    42241 agaccggtgg agttggtgaa tccactgatg ttgggttgca cgaaataata gcggaggaat
    42301 ccattgggat tcgtcgtcag ttgccctaaa gccattgcga cataatccgg actcagcgct
    42361 gtaagggttg ttggaaagga atgggtttgc cgcacgtaga gttcaactcc ttgggcaatg
    42421 gtcgccaatt gtccatcctc cgaatcccgc ctcacggacg ccagttgatt gatcacattt
    42481 ggtgcgatga agctggcaag aatggcgata atggccatga cgccaatcat ttccacaagc
    42541 gtaaatccgc ttgtggctag gcatcgtgag gtgcgaatga atgcggacat cctccggcca
    42601 ataatttcaa ctgcgttccg gttgctgcat tgactgggct ggagcgttgt gccgggacgc
    42661 cagtctgttt tgaatgagat tgaggccgta tggcgcccta tggcctgagt aagaaagtca
    42721 ttgcgtcttg tcatgaatcc gatcctgtct tgctccttct taaagaggct cctgcctggg
    42781 gggcgtgtga gtattcttcc ctccaacggg catatccagc ctggtagtcc accaaccctt
    42841 ctttcggtgt ttgaaaagcc aatcttaatt ttcttaaact tttcaaatgg tcatggtggg
    42901 tttgcctgga tcacaggagg caggaggatc gggtggaagt caggttatgg agatgggaaa
    42961 tgccgtcatt ttttcccggt catggggcgg gggtcttgac gatgtttcga cttaacaggg
    43021 cggtgacaga ggattccatc gtttggtgaa gacgcacaag gggtggaacc aatagctggt
    43081 attggtggtg agagcgaact gcacgtcagg gggggggttg tagctatcgt cctcgtccat
    43141 accaatttcg gtgcctataa ggtgaaatct gctatgcgtg ggaagaaggt cgccgttgga
    43201 atgcgttggt tggtggtcaa tagaaaaact tcccccattg cctttgggaa tcaggctcac
    43261 ggaataaaaa agatggctaa tcgtcccacg atgaatagtg agggcgggag ttatgctgtc
    43321 attcgtgttc caccaggttt caaaagagac gggagtcgtg atggatgggg cggcatcttg
    43381 tgagagattg gaaaggagaa gaaaacgggc attgatgatt tccgcgccag tcagtccagt
    43441 cgagttctga aatccggcta tcgaggggtg caccgaataa tagcgtggga acccccgtgc
    43501 atttcgcgtc agttgggcta gaggcaatgg cacatagtca ggggaaaggg cgagtaacga
    43561 aggggggaac gtgtggtgct gtcgaagata ggcgatcaca ccggttgcga tcatctgaag
    43621 gtcacgggtc tcacggtctt cactattctg atcccagacc ctgaccatag agggcgatac
    43681 aacagccagc cctatagata tcatggccaa caagccgatg aggacaatga gggtcattcc
    43741 ggcttccggg tcagaggtaa tctggtggtg agttgaggac cgtgcagaga cgccaggaga
    43801 gttcatgagg cttgtcaatg aattccaccc attaaactca tcatgggcaa aaagagggct
    43861 agtgcgacga tacccacgat cccgattaat cccagggtga tcacgggctc aagaatgcca
    43921 aagacttttt tgattcggcg cgggatttca cgattataat aattcgccac gttcatcagt
    43981 gtgttgggta atgtcccggt ggcttccccg actgaaatca tttgaatcac cattggcgga
    44041 aagatcggat gacggtttaa ggattcattg atggtggagc cttcggacac agcgcgttgc
    44101 gtgtcctgta gcgcccgttc catcacggaa ttgccaacga ggccctggca cagttgcagg
    44161 ctttgcagga cagggattcc ggctcgatac aagaccgcaa agttttgaac aaacttcgat
    44221 atcgtgagca tccggatgag ttcgcccagg acggggagtt gtaagatcat gcggtcgcgc
    44281 cagtgagcaa attctgggag gtatttttgt cccaaaaccc acaccattgg gccgagcagg
    44341 aggaatgaac tccagagcca ccaactcccg acgaagagtt cgctgagggt gatgatgata
    44401 agggtgatag taggaagggg cacatgcaga ccttccagga tagggacaaa tcgcggaatg
    44461 acgaaggtaa agagcaagac cactaatccg atcacggcta tgatgacgat actcggataa
    44521 atggtggcct gacgcacatc tccacgaatt tgatcaagcc aatccagata tcgttctaat
    44581 tctttgaagg tatcggtcaa ggtgccactg tcttctcccg catgcaccag attggtgatt
    44641 tgcggaggaa aaatcctggg gtgttgcttc atggcatcat gcaatgtatt cccggcttcg
    44701 acttgttgga aaatggcttg taaggttgcg cgtaaatgaa gattgggtgt ctcgatcgac
    44761 aaacttttta acgcctgggt taggggaatg ccggcctcga ggagtgtaaa catgtgtgtg
    44821 gaaaattcca tgagatcacg gggtttgact ctccctcttt tttgagtggt tcgtgcgagg
    44881 gatttcggtt tggattcgaa tgtccgcacc agccagagcc ccatgtcctg gagctggcgt
    44941 tccaggtgga aaagatccgg cgccgtcatc tctccctcaa ccggcttccc atggtcgtcg
    45001 atggcacggt attgaaaatt agccaccggt gaccctcaac acttcctcga tggtggtaat
    45061 tccttgaagg gctttcagaa gtccgtcatc aaacatggtt ggcatctgat ctctccggat
    45121 gatggcccga atccctgcca tatccggtcc gtgcacgata ggcgcatgga gttcctctga
    45181 aatcgggata atttcgtaaa tacctgctcg gcctttatac cctgtctggc tacatgcgtg
    45241 gcatcccttt cccgtccaaa ggctaaggag tggaccttcc ggtaattcta ctcgaagatc
    45301 cgcaaggatg gtggaggcat ctgtccgttc aaccttgcat tccggacaaa tacaccgaac
    45361 gaggcgttgt ccaataatgg ccgtcagggc ggaggcgagg aggaaggctt ccacacccat
    45421 gtcgattaaa cgaggaatgg ctcccagtgc atcattggtg tgaagagtgg aaaaaaccaa
    45481 atgtccggtt aaggccgcac gcatggccag atcggccgtt tcctggtcac gaatttcacc
    45541 aacaagtatg acgtccggat cttgccgaag gagggcacga agcccggccg caaaggtcat
    45601 cccgatgtcc ggattgacct gcgtttggcg aatcagaggc atctgatatt cgatcggatc
    45661 ttccaacgta aacacacttt tttctttcgc atcgacatgg cggagcgccg tatacaatgt
    45721 cgtggtcttt ccactccccg tcggacccgt cacgaggata atgccatggg gacgttggat
    45781 cagtgcttgg acgcgcgatt ggtcgttggg tgtaaatcct aatgccagga attcaagctt
    45841 taatgcccct ttatccagaa ttcggaggac aatgctttct ccgaaattcg ttggaa
//
LOCUS       DAORAQ010000004        43915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_193_length_43915_cov_62.901961, whole genome shotgun sequence.
ACCESSION   DAORAQ010000004 DAORAQ010000000
VERSION     DAORAQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 43915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43915
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193_length_43915_cov_62.901961"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKM72_00795"
     CDS             complement(<1..1033)
                     /locus_tag="PKM72_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59339.1"
                     /translation="MIAQGKSGQIITIAGTGEPGYEGDGQPGTRAVLNEPKNVCFDAE
                     GNLLIADSENHVIRRLIHSTGVIETVAGCVGTRRAGEGNPETPMPSPISQVEEEIDPL
                     ADVAEIPGQAYSQTPDLSGTVRYIVGKGIGKDRFAGDGGLATSAQLNFPSSVAVDAAG
                     TLFIADTWNHRIRRVDAKTGLIQTVAGNGQSKWTGDGGPGISASLNEPVALVVDGTRL
                     YIADQSNNRIRMLDLGSGVITTIAGTGDSGYTGDGMPAVESALAGPSGLALDHDGNLY
                     IADTFNSRIRMLDHNTGLLSTVVGDGAEFRYERGVNEQSQALARPYGIAITPQGHVLI
                     TDSDHHLIRK"
     gene            complement(1037..2110)
                     /gene="leuB"
                     /locus_tag="PKM72_00800"
     CDS             complement(1037..2110)
                     /gene="leuB"
                     /locus_tag="PKM72_00800"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNP59340.1"
                     /translation="MKKRIAVLPGDGIGPEIMPQAIRVLEAIGQKCGHQFTFTYGQVG
                     GEAIDATGFPLPAETLKLAQSCDAVLLGAVGGPKWEGLEYERRPERALLGLREQLGLF
                     ANLRPAKMYSALIGSSSLKPEVVEGIDILVVRELTGGIYFGKPKGIEQTVTGHRGFNT
                     EVYTTEEIRRIADVAFQAAKKRRGLVHSIDKANVLESSELWRREVINVHQSYPDVELR
                     HMYVDNCAMQLVRYPKQFDVVVTTNLFGDILSDEAAMLTGSIGMLPSASVGATVGMFE
                     PIHGSAPDIAGKNLANPIGTIASAAMMLSYSFELTKEAEMIESAIQRTLEQGYRTKDI
                     ESQGSTLVGTAEMGNHILKQLGH"
     gene            complement(2259..3806)
                     /locus_tag="PKM72_00805"
     CDS             complement(2259..3806)
                     /locus_tag="PKM72_00805"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNP59341.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNIEEKILVAKQLARLNVDIIE
                     AGFAFSSPGDFEAIHRIAQEVEGPVICSLARAKPEDIDRAWEALKGAPKCRIHTFLSS
                     SDIHLKHQFRMTRDEALKRAVEMVRHARGYVEDVEYSPMDATRSDLTYLCEVVEAVVE
                     AGAGTVNIPDTVGYTTPEEFGKIIRTLRERVRGMETAIISVHCHNDLGLAVANSMAAI
                     YAGAGQVECTINGIGERAGNASLEEVAMGLRTRTDFYQSDTRIRTEEISKASRLVSNI
                     TGMVVQPNKAIVGANAFAHTSGIHQDGLLKDKSTYEIMRPESVGLTQSRLVMGKLSGR
                     HAFREELANLGYTLSDQELQEAFERFKRLADQKKELFEEDLETIVNKAITKVPERYAL
                     KGLHVESGLEQRPSAMVELIMDGRIEKRTGTGDGPVDAVYRTIAALTETKSLLRAYIV
                     KAITGGTDAQGEVSVRVEENSETVTGHGSDTDIILASAQAYLNALNKLADLAERQAKA
                     EQKIGLC"
     gene            complement(3869..4639)
                     /gene="pssA"
                     /locus_tag="PKM72_00810"
     CDS             complement(3869..4639)
                     /gene="pssA"
                     /locus_tag="PKM72_00810"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNP59342.1"
                     /translation="MKQPKKRRVVYLIPNLLTTGNLFSGLASILFVFGGDYGPAAIAI
                     LVAIVFDVLDGTSARLMKSTSDFGLQYDSLADVVAFGVAPGLLMYSWALSGPKMLGAA
                     VMFAFVACGALRLARHNVLATTGGDGKPFTGLPIPGAAGVMATLVIFDHHVAPLGEKV
                     KPILGIVLTLGLAFLMVSTFRYRSLKELKTQEHHHFNYLVYAVLILMAVLAYPQAMLF
                     VVFAAYALSGVLEQGWKLVAGLMGKKPTEEVGTDGLHK"
     gene            complement(4636..5301)
                     /locus_tag="PKM72_00815"
     CDS             complement(4636..5301)
                     /locus_tag="PKM72_00815"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNP59343.1"
                     /translation="MADQAKGIPVAKEGLPFIAGGGLLTLGCWVAGPVWLTCLMGSLT
                     LFTAWFFRNPARTIPQGRNLIVSPGDGTVVAVEQEFEHRFLKEPSIRISIFLNVFNVH
                     INRLPATGVLQDVVYAPGKFLVASRPEATVENEQNALMLKRSDGKKILCVQIAGLIAR
                     RIVCWVKPGEQVEVGERFGLIRFGSRMDLYLPHDSAIQVKVGEKVKGGSSIVAELPCL
                     EPS"
     gene            complement(5335..6351)
                     /gene="ilvC"
                     /locus_tag="PKM72_00820"
     CDS             complement(5335..6351)
                     /gene="ilvC"
                     /locus_tag="PKM72_00820"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNP59344.1"
                     /translation="MMKIYYEKDADRQILAQKTVAVVGFGSQGHAHALNMRDSGLQVV
                     VGCREGSSWNKAEAAGLKVMPTADAVKGADVVMLLAPDEAQAAIYNKDIGPNLKQGAY
                     LAFGHGFNIHFGQIQPASHVNVFMVAPKGPGHLVRSEYTKGTGVPCLLAIHQDPGGQT
                     TKVGLAYACAIGGARAGVIETSFREETETDLFGEQAVLCGGLTSLIQAGFETLVEAGY
                     SPEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRVVTDETKKVMKE
                     ILGEIQSGRFAREWVLENQANRPVYNALLRRGEEHPIEEVGSRLRGMMPWLQKDQLVD
                     KQKN"
     gene            complement(6445..6966)
                     /gene="ilvN"
                     /locus_tag="PKM72_00825"
     CDS             complement(6445..6966)
                     /gene="ilvN"
                     /locus_tag="PKM72_00825"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNP59345.1"
                     /translation="MEHIISLTVENKFGSLSRIAGLFSGRGFNIESLSVAPTLDPSVS
                     MMTVVTKGDDPIIEQILKQLNKLIDVIKVVDISESEFVERETALIKVHTRAEDRAEAL
                     RIADIFRANVLDSSPTSYTIEVTGDVRKVQAIINLLQPLGIKELVRTGRIAIGREPTR
                     STQTQSRPLAKAN"
     gene            complement(6989..8764)
                     /gene="ilvB"
                     /locus_tag="PKM72_00830"
     CDS             complement(6989..8764)
                     /gene="ilvB"
                     /locus_tag="PKM72_00830"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNP59346.1"
                     /translation="MKITGAEIFLEALKREGVKTMFALPGGVVLKIFDVLHQQKDIRV
                     ILTRHEQGAGFMAVGYAKAMGKPGVALITSGPGMTNVITSLADAYMDSVPIVVFSGQV
                     PTALIGNDAFQEADNIGLSRPCTKYNFLVKDVNDLAQTIKEAFYIATTGRPGPVLVDI
                     PKDISLDKAEFHYPTSVSIRGYNPTYDGSRWKIKQAADAIMKSKRPILYVGGGVVLSG
                     ASHEVKDLAELTQIPVDMTLMGLGAFPGNHPLSLGMLGMHGTYVANMAMHYSDLVIAV
                     GARFDDRVTGKVSEFCPDATIIHIDIDPTSIRKNIQVDIPIVGDCKRVLTELNNILRA
                     TVNGNQKEQRKPWWDQLNAWREAHPLHYDQDPDGLIKPQFLIDRLYHLTSDRNPILAT
                     DVGQHQMWAAQYFKLQNPQSWLTSGGLGTMGFGLPAAMGAQAAFPDRLVLCVAGDGSI
                     QMNTQELATAVVEQLPVKVFIINNRFHGMVRQWQDLFYEGRYASSYLGTVPDFVKLAE
                     AYGATGIRIEKVSEMDSGIREALSIKGPVVIDVPTYQFENCYPMIPAGGCNHEMLLGD
                     PPDLKKPNAMQKIGTRADSDSVITA"
     gene            9024..10184
                     /gene="bioF"
                     /locus_tag="PKM72_00835"
     CDS             9024..10184
                     /gene="bioF"
                     /locus_tag="PKM72_00835"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNP59347.1"
                     /translation="MFQEELQTRAQQHLLRELSLINSPSNPVISVEGREVLLFASNNY
                     LGLANHPKVKEAAVNAIEKFGVGSGASRLISGTITPHHVLERELGSFKNAEAALTFSS
                     GYATNIGIIPNLATADSLILADRLCHASLIDGCRLSRATLRVFHHNDVGHLKRLLSGP
                     RSRTSTLILTEGVFSMDGDMAPLPEMVRLAEEYGATLLIDDAHGTGVMGATGRGTVEH
                     FGLDPRRILQMGTLSKALGGSGGFVAGPKDFVAYLVNTARSFIYSTAPPPAIAAAASA
                     ALSVIQEEPQRRAKLWCNREFLHHGLVAMGFELTNTQSPIIPIMVNDPELGVQMSQHL
                     RAEGVWIPAVRPPTVPKGTSRLRITVTADHSPEHLETALRALRKVGTAFKVL"
     gene            10457..12352
                     /locus_tag="PKM72_00840"
     CDS             10457..12352
                     /locus_tag="PKM72_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNP59348.1"
                     /translation="MNTQLLPGKIEIQGEIAKGQTGTIYYGYDLELRQEMAIKVYHTH
                     INGRLIPGRAFIEKAQSLLRLEHPNLIKIFKVEEQDGTPIVCMEFFDAPSLQHVIQSE
                     GPLSVEKMLMLAREIAEVLVHIHFQGIIHGTLHPGHVLVGPQGQIKVMDFGLSWILMD
                     ILKNCDAELLRPLPYLLPETAKSELLTLGSDLYCLGFMMYDMLTTRVPYSGLPKTSIM
                     GKLAFDQADPVFDFPDHVPKAICELIRQMTRNQSSQRLQDATHVLTILNQQLAKFAPD
                     VLRPPVAPAEPQPTPEIQTTKVEAPSLPPQAPVSVPSSNQPPAVTRPKHSTDYQGIHQ
                     AKTRKKLGVTIGISLFLLLGGTMGYIYKERLGIPFLTSPEFRVPEQPRINEPAPSVPP
                     IDSFTTMTPEPPISPPPHSSQAEEPRLNTNDPSKGDPSLRTTPPPMPTPVVTKDHPLQ
                     PDVSQHPGTPLPSTLERTRTQPQNLSPQPKPEAKPVPAQPHSTPPQDNSTIGNPPLLK
                     KPTTPVTLPAPTPKPPAIVSETDQAGVPPDPDKHSSHSKESSEVSHTGPQPIQDSTVL
                     SPNAESLDNIESKELKDILNSVQSPETVPILSKDGPGTLLPSAPNPPPLSPSPKLPAL
                     SSPVSPP"
     gene            12455..13981
                     /locus_tag="PKM72_00845"
     CDS             12455..13981
                     /locus_tag="PKM72_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNP59349.1"
                     /translation="MLAKIQSVGLVGLDANLIEIEVDIGGGLPQFSIVGLPDATVRES
                     RDRVRSALKNTGFHFPAKKITVNLAPAGLKKEGAGLELGIAIGILVAEGVLSQEEVMS
                     YIFVGELALDGRIKAVPGALSMAIATHHPFSLILPHDNASQAAVVDHATVFGVSTLPQ
                     VVEFLQGSLTFLPAKNTFSIGQAGPSSIEEDFADVVGQYQAKRALEVAAAGGHNLLMV
                     GPPGSGKTMLAQRLTGILPPMSFQECLEASQVHSVAGNLPPHAALLANRPFRAPHHTI
                     SEAGLVGGGSIPRPGEVSLAHHGVLFLDEALEFKRSLLDSLRQPLENGTVTLTRAHAS
                     LTFPARLMLIVAMNPCPCGYCGDPTHECVCTPYHIQRYRSRLSGPLLDRLDIHIEVPA
                     VPMKELSGRTIGESSSKIRDRVIRARHCQIERYSAEQTLNNAQLKPRLLKKYCQLDQA
                     GNTLLDQAVTRLGLSARAYGRILRVARTIADLGESVMISSTHVAEAIQYRTLDRPILA
                     "
     gene            14000..14215
                     /locus_tag="PKM72_00850"
     CDS             14000..14215
                     /locus_tag="PKM72_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59350.1"
                     /translation="MESFKVFRWWFIIGSLMALAVIMIQGGVRDLMLANEPIWEIKLV
                     ELGPPIFGGGLLGGCIALILNRIKDKS"
     gene            14618..15052
                     /locus_tag="PKM72_00855"
     CDS             14618..15052
                     /locus_tag="PKM72_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59351.1"
                     /translation="MDVKVGPHWYRNSNGIVEVDGLPQIEMTLRQTGKTPVRVNFAIF
                     DKVGKIHAKIEANSLVINEQGAYHLERLETGLVLSNRGDQKRVLAINVTGTDQVEIPE
                     GEFYTLKGHLLKITPNEWSVEKTTERSGETDMKGQAVAVGSQ"
     gene            complement(15378..15680)
                     /locus_tag="PKM72_00860"
     CDS             complement(15378..15680)
                     /locus_tag="PKM72_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59352.1"
                     /translation="MSSWTEPGTVSTISLISMAKGVDVSLLDTVSFQHITRILEVIDD
                     LDISREAVEIPLSPASPGVVRRLPNGKLEIVVDADLPFDEWVQTLPEKVRAQMPDD"
     gene            complement(15694..16734)
                     /locus_tag="PKM72_00865"
     CDS             complement(15694..16734)
                     /locus_tag="PKM72_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNP59353.1"
                     /translation="MQKIDLRSDTVTKPTPAMREAMAQAEVGDDVYGEDPTVNRLEAM
                     AADMLGKEAAVYVPSGVMGNQLALRLHTRPGDEVIVDSTSHLIRYEGGSASSLSGVQL
                     CCVPGDRGRLSPELVKAAIRPKGLHNPPSTLVCLEQTHNVGGGSIYSLETINRIAEVA
                     RTHGLALHLDGARLFNAVVATGVAAADYSRPFDTVSFCLSKGLGAPIGSLVVSDTARV
                     QTLRRLRKVFGGGMRQVGILAAAGVYALENHIARLAEDHANAHYLATLLEDIPGVVVD
                     VKAVETNMVMFQVPRSLKTTEVLLADCLEAGVLLNAMGDRAFRVVTHLDVNHEDMVAA
                     GRIFRRVFSATE"
     gene            16835..17689
                     /locus_tag="PKM72_00870"
     CDS             16835..17689
                     /locus_tag="PKM72_00870"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP59354.1"
                     /translation="MKPRLLVTGGAGFLGNHLLRRANQFVAAGTLHATPSTSLPGVTF
                     HVCDLQSPEEVRVLMDRVQPDVIIHTACSDQGKGIEAILPAAGLLAMQSVERNIRFIH
                     LSTDQVFDGTSAPYTEESPTNPINPYGKAKAQAEELIRSLNPKATIVRTSLLYDLRTP
                     DRQTTRLIESTKTGEQYRLFIDELRCPIWVENLAELLLEVATKNIPGILHLGGPDSLN
                     RWELGMGLLRHFGVTQTLNIQKGTIEESGLCRPKNLTMDSSRSERLLKKPRVSLQEAR
                     KMTVTSER"
     gene            complement(18426..18743)
                     /locus_tag="PKM72_00875"
     CDS             complement(18426..18743)
                     /locus_tag="PKM72_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNP59355.1"
                     /translation="MRHHESDVVDGKMLGVFAMGLIIGSVATLLLSPQSGRDSREQIR
                     DYVRRKGEDWSDLQEKTVETYQDIVSGTHKGLAEIQETIREAVHAGMDEFRKEWKDVK
                     NNR"
     gene            18868..19017
                     /locus_tag="PKM72_00880"
     CDS             18868..19017
                     /locus_tag="PKM72_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59356.1"
                     /translation="MSQKLGNVMMHHSNRVTKVLFVAEDVSDHITDHHVSLRKKNRDF
                     DNYAT"
     gene            19771..20067
                     /locus_tag="PKM72_00885"
     CDS             19771..20067
                     /locus_tag="PKM72_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNP59357.1"
                     /translation="MNKQLITGVMAIAFTLSVNPAWALFGDDKAELLQGTKVTLVEAV
                     QTALTNVKGKAFNAELEKEDGKTVFEVKVIDETGAAREIYVDAQTGEVLKIEKD"
     gene            20623..21672
                     /locus_tag="PKM72_00890"
     CDS             20623..21672
                     /locus_tag="PKM72_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNP59358.1"
                     /translation="MKDIQTHDRHRLAEKIRFLERDVIEVITTTFLQQHPETKELGEV
                     AKNHCITDARYHLTFLRTAIEFGNKDTFRQYLRWTTKVLQTRNIPQHVLQRFLEHISH
                     ALEPHLTEPEKTVVRSHLLETPESEPPEPQTSARSTTPLALHQEVFLQALLAGDRHAA
                     RTIALEAFSNTRSLSDVYVELFQESLYKIGSLWEANHISVAQEHVATAITQYVMANLY
                     TCVTPAQPSQGKGIVTGVEGELHQIGPHMVADMLEMQGWDIRFLGVNVPHQSILQLIK
                     DFRPTLVGISATMVLKVPTVRDLIKDIRNHFPSAATPRILVGGSAFRALPDVYRDIGA
                     DAFAYDLRSLTALLP"
     gene            21686..23164
                     /locus_tag="PKM72_00895"
     CDS             21686..23164
                     /locus_tag="PKM72_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNP59359.1"
                     /translation="MVLVISRFKVANGLEGDVVEAFQKRPRLVEAVPGFLGMEVYTPH
                     SDQTIFHLVTRWTDRETFHAWHKSPDHRLSHGMLPTGLKLDPTVTQILELERLPQTGL
                     QGGLQDFLGDSILLLEKFLNQTDTIVYCVMTPDGRIQVYNDVLLTKLNVSRAELQTKF
                     IWEFLTDPDQKHLRDILEDGISSHSNRMRLNFLDSSGIPHTLDCLVQKNSADTTILIG
                     EEPEKFNQRAFVELMQIQNEMTVKMREEVKSGNKLSENNSYLNQLVTERTKDLVYHQE
                     QLRAMLVKLALVEQSERQNLANDLHDYLAQMLIACRLKLGQIDHEHTLPSHSKVIQEV
                     DGMIDQSLTFTRTLISDLNPSILQHHGLLTALKYLSEDMQRFGLTVASPETILPVKVA
                     EDISIVVYKTVRELLFNVLKHAGVTTASIHWECPSEHVLLIRVSDSGKGFDPETRQLE
                     QERGHLGLFFYRERIRALKGQFDITSSAKMGTTITIIVPPSP"
     gene            23473..24132
                     /locus_tag="PKM72_00900"
     CDS             23473..24132
                     /locus_tag="PKM72_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP59360.1"
                     /translation="MNTLKRARLFLVDDHILVLESCKKLLEPHHDIVGEAHDASDLVS
                     QIRRAKPDILLMDISMPDQNGYEAARVIKIACPSVKIIFVSMHLEPTFIMEAFRAGGE
                     GYVPKQTAGSELLSAIQQVQEKQRYLSPLIPEEVQYAVLAQMDGMPGTELSGKLTPRQ
                     QEVLKLVAQGFSAKDIANVLTISQSTVAFHKMQIVQALGLHSKADLTKYAVKLGISPL
                     D"
     gene            complement(24250..24903)
                     /locus_tag="PKM72_00905"
     CDS             complement(24250..24903)
                     /locus_tag="PKM72_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP59361.1"
                     /translation="MSKTRVLLAEDHVLVSEGLTKLLETDFTLVGTVVDGHALVQAVK
                     KHTPDIAIVDISLPLLNGLEAARQIKKCEPQTKIIFLTMHSEDHFVQEAFKAGGAGYI
                     LKQSATAELVFAINEVYQGRTYVSPSIAQGIVSQALNPSSNSKKTAQPETPPLTQRQV
                     EILQLVAEGKSNKDIAVVLNLAVKTVEFHKTRIMQILGLKTASELTKYAIATGIISI"
     gene            complement(25303..25926)
                     /locus_tag="PKM72_00910"
     CDS             complement(25303..25926)
                     /locus_tag="PKM72_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNP59362.1"
                     /translation="MKTKNDKNYPVKEFPLKGLTGISDKTLEMHFKLYEGYVKATNSL
                     TRQIGEFLIDGQVDQEEMPAYSELTRRLGFEYNGMVLHELYFENLSSQESKGPQQGQI
                     SIQKAIEDSFGSFDIWRADFSSVGKMRGVGWAVCNINPANGRVSNHWVSLHEFGNVAG
                     FRPLLVMDVWEHAYLMDFKPSERGEYIERFLAHVNWDVFAKRLEGQS"
     gene            26168..26683
                     /locus_tag="PKM72_00915"
     CDS             26168..26683
                     /locus_tag="PKM72_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59363.1"
                     /translation="MNIQFTISDEMGKELTALQSALGMKGVEDLTIHAWNVLGFLIET
                     IRNEKGPLSPEAIHWISQPTMVSSPTETLQEHAALSHAEIDTRILEEIHRLLDDDQLD
                     QALKATHILKILKSHAVGRSTNHQVKSEMLLAAAETFAAECDPEKAAQLIIDATHEHH
                     LNTTDGSTDRG"
     gene            26979..27314
                     /locus_tag="PKM72_00920"
     CDS             26979..27314
                     /locus_tag="PKM72_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59364.1"
                     /translation="MLSNEPKFLITAKIKEFSIPPITDGLIIGKDAAIGVSALQKALR
                     LLIPEEFDHIEIQDEVIHSMLIRSSITRRLTQKRVVTFLLDQVKPLMVADDILHIQLD
                     TEITITQEL"
     gene            27324..28511
                     /locus_tag="PKM72_00925"
     CDS             27324..28511
                     /locus_tag="PKM72_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59365.1"
                     /translation="MVHLTAQDAVSPSVLMVSAEMGLRDLNLNHKLQSYEAYAVFSEV
                     VNQKPSEQFLGIVPKHMIGRYPYRIFGDLLSCSTSTQVEPSTPLDVLYADFQRHHIDA
                     FSVIDTGRGFIGAITPTSLLETLWHREQFLTTRLQKEINEKNFAKRELQQAKDELDNH
                     VGSRNNLVFDHTQQLLTLSESLISTENRERRALAGELHDHLAQMLAVGRIKLAQGSHL
                     TSDPEVLSLLNTVDQFLQESLSYTRTLMTELNSFNLHQSGLLKVLEELTKKMKLLGLV
                     VTLEAERPLPDLSENQDFLLYWSLRELLFNVIKHAHVEQATISIHQLHGTHLRCMVSD
                     HGCGFDPATMAGEIPGQDHFGLSGIRGRMTALHGTISLNSYPGQGTRVSLTIPLSPPS
                     PPH"
     gene            28692..29297
                     /locus_tag="PKM72_00930"
     CDS             28692..29297
                     /locus_tag="PKM72_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNP59366.1"
                     /translation="MKSPFKYFVVLVTCPLFIATIMENTEGQVLVVPLAGFEETISPE
                     THVPRSDEDNRRGHSDEELEYRIQRRLEISPYRSDGITVQVKQGKTVLSGHVKDRDSI
                     VDVVEIAYDSGATNVDNQIQLDHPWREINDQKLKEAVEAELYLSPFVNSVPIRVEAQS
                     GTVTLSGRVENRGEIVDAVKNAYEAGAKNVRIRLWIDPTLD"
     gene            complement(29449..29862)
                     /locus_tag="PKM72_00935"
     CDS             complement(29449..29862)
                     /locus_tag="PKM72_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59367.1"
                     /translation="MKKTAVADQVFRVSLPCLGFMLVVMFPLAEPVFGDDSNEAPADE
                     IHSKDRSENPSPSIGDKTKDSPILGEGVRTPPDIGASGTASSTPPVGTESPTDDPQIL
                     PDISPVPTHPLNSEMAPINESEPLGQPNHLKNTPK"
     gene            complement(29931..31502)
                     /locus_tag="PKM72_00940"
     CDS             complement(29931..31502)
                     /locus_tag="PKM72_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59368.1"
                     /translation="MDIYSTVSQVTWRMSRSLIMGCILIASSGLGMGLAADQGKPKSW
                     EAVPGSGKLVPQIGPPAVVKDWQRAYPQYQPVGNGDTQESPDAKPGTPNPPAIHTFGG
                     RTPSSFVFDLSPDEPLEKVIERETQQRSKIRSQQQDLLEERYDLSPQFVGGVTMTRGK
                     PVPKGPTARLKKGLTFAQLARMPEEDIKASDLFPYLPLPHPLHSTGGMVFPSMMTDIH
                     PERERFDVVHDLPDHFLPEFPPPLFLTSRPDLGDVSQGQEITNENFYALFDGILSPVQ
                     FDGVRLLVEKIPQQQFNATDDRKTVPPSRGVACFDCHVNGHTNGAIHLNPDNRPQFSR
                     FRIETPSLRGVNIQKVFGSKRAMTSVEDFSEFENRSAYFDHDLATAEKKGRRDLTRTE
                     VMKMAQFQKLVDFPPAPKLDLFGRLNPKKATEEELRGEALFSGKAKCVACHTAPYFTD
                     NLMHDLKVERFYHGRAEGSIKTFPLRGIKDSPPYLHDGRLLTLEDTVEFFALVTGVQL
                     TKEEKADLSAYLRTL"
     gene            complement(31586..32056)
                     /locus_tag="PKM72_00945"
     CDS             complement(31586..32056)
                     /locus_tag="PKM72_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59369.1"
                     /translation="MPFKELTLLAVVLGSLFLGSIGGMLLASPNPTESVLTSENSTKP
                     ISTSALNIVNEEGKVRATLSLWDGVHPALIMGDNHCDRRTSLAVYGKERTGLTLYGED
                     CKRRVALEVQSDDLPTFVLRDYLDNPRVQVVLNKDGSPLVRLFDENGKALWEKP"
     gene            complement(32107..32259)
                     /locus_tag="PKM72_00950"
     CDS             complement(32107..32259)
                     /locus_tag="PKM72_00950"
                     /inference="COORDINATES: protein motif:HMM:NF023180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HNP59370.1"
                     /translation="MSTIVLVILILLLVGALPVWPYSSSWGAYPSGGLGLVLVIVLIL
                     ALTGRL"
     gene            complement(32282..32467)
                     /locus_tag="PKM72_00955"
     CDS             complement(32282..32467)
                     /locus_tag="PKM72_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59371.1"
                     /translation="MNKILEEDMPGINGSGQSGKSRCFFGDWSRRTLLKTALPMKDWP
                     RSVHVSEETMSTDHTIS"
     gene            32984..34447
                     /locus_tag="PKM72_00960"
     CDS             32984..34447
                     /locus_tag="PKM72_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59372.1"
                     /translation="MTLPQDGTQTGTRSPKWDVSVLIAICALIGAIFILDLYTKTGVA
                     IGMLYVSAIMLTPWLPHEKSPFIVAGICTIVAIIGVVYSPGVNVVHSGSTIAGNAVTN
                     RLLSLFMIWATALLTYQYRQGMEVRLRLGSIVESTHDAIIGQTLEGKVTNWNNGAEQM
                     FGYSPQESIGQYMTFLFPPDRIPEEKDILEKLHGGKQIKNFETIRRRKDGQEIPVSVT
                     ISPIIDRWGKVIGASKIARDISQQKGLENLLAIQNLTLASHAASLKQSNEDLEQFAYI
                     ASHDLQEPLRTIHGFTQLLAERYKDQLDDQGREFIGFVTDGANRMQTLIQDLLKYSRI
                     QAQELNTVQVNGEEVLQEILEHLLMVIEDKQASITHDPLPIIQTDRSHFQHLLQNLIT
                     NAIKFHGPKPPRIHLSAQKKTNEWLFSVQDNGIGVEPEYFERIFLPFKRLHTREEYQG
                     TGIGLAICKKIVERRGGRIWVESQPGNGTKFSFTIPK"
     gene            34462..34905
                     /locus_tag="PKM72_00965"
     CDS             34462..34905
                     /locus_tag="PKM72_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59373.1"
                     /translation="MQNPIEILLIEDNPADIRLTQEAFRAARLHNTIHVAQDGVSAMS
                     FIRQTAPFQDAPRPDLILLDLNLPKKDGREILKEIKSDPHTRTIPVVVLTTSEDEGDV
                     LRSYDLHANAYLVKPIDVLRFIKMIQSLESFWLSVVKLPPKVPEL"
     gene            34902..36884
                     /locus_tag="PKM72_00970"
     CDS             34902..36884
                     /locus_tag="PKM72_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59374.1"
                     /translation="MSDLHSPTHSILLVEDNPADAELVRERLKDASGMSPFYMTRAST
                     LNEALTTLQNTSFDVILLDLNLPETNGLETLKRIRSANSVIPLVVLTVNEDEPLALEA
                     IKLGAQDYVPKSSMNAVLLSRILHYAIEREKNDRIRRDSERKYRLFVDGATGLAFVML
                     DLHGNITHWNSGAERLFGYPEDAALHKHFSMLFTDEDQRNGRPENELRRAEAVEKGDD
                     DNWLVRADGSRFWASGAVTAIRDSTDHLIGFAKVVRDKSAQKDARDKLAELNRTLEER
                     VIQRTQELTRNQERLRAMASDLTVTEQRERRRLASDLHDYLAQLLVVCRLKLGQGQSL
                     TDTESLVKVIQEADALLDQSLTYTRTLVSQLSPTCLYEFGLYAALVWLGSEMRKQGLT
                     VNVNGANLSLNLPEDQAVLIFQSVRELLYNALKHSGVNEVTVNLAMQGNNHLIIEVED
                     FGCGFIDNAEDRNEQSPTNFGLFSIRERLEALGGEMVLNSTLQKGTHVLLRIPVEKGI
                     EEFRPHTARPTSVRAVEQTPSLRSHSEKIRILIVDDHKMVREGFCHILNSQLDFEVVG
                     EAEDGIQAMTLCESLRPDVIVMDLHMPNMDGLEATRRIKENIPETVIIGLSVYDTPDV
                     ARWFQEAGAETFVTKGGPAESLVTMVRKSWHQKKPA"
     gene            complement(36896..37615)
                     /locus_tag="PKM72_00975"
     CDS             complement(36896..37615)
                     /locus_tag="PKM72_00975"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59375.1"
                     /translation="MKQFIRIGLIFRVLAWAIPVVWFCSFPRLAEAAPSFWQQQTVTF
                     LGTTLNQHHQPKGIVGKLEIAFRKQEGQEVLNVSFSSGPGKFSPFTQVAIQSGIVAAA
                     RVAGLDPKTWDIFLKFPNPGVTIYGDSLTAMVSLVVLAMANDNTILLSRVMTGKVTRD
                     GHIGAVGGIPLKIQAAYAEHIRRVIIPEERLPEDNDWQTPFLMQISPVNTVFQAYQIL
                     TGANLPNVEKAGGRMAKNENE"
     gene            complement(37619..37999)
                     /locus_tag="PKM72_00980"
     CDS             complement(37619..37999)
                     /locus_tag="PKM72_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59376.1"
                     /translation="MRNNKPCFHWIFGVVFCLLFGGNGMAADMPNEPGEANLIHEDVN
                     IITGLYIREYSLKGDGIVDYKTARQIIFFENNKYWNTVIETVEWPLFYWIDENRDGVF
                     DHFVDQRVEGKREYIIPYLPVSGN"
     gene            complement(38111..38416)
                     /locus_tag="PKM72_00985"
     CDS             complement(38111..38416)
                     /locus_tag="PKM72_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59377.1"
                     /translation="MQTLTLEAMRTDHPDVFPQLMNQMQQSHCSRCGGTMIEETCMDI
                     FSDYEDFQFQAKHCIQCGDVIDPFILLNRLLKNQTETPALNSFPQARPRVNGLVMTA"
     gene            complement(38933..40444)
                     /locus_tag="PKM72_00990"
     CDS             complement(38933..40444)
                     /locus_tag="PKM72_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HNP59378.1"
                     /translation="MNQHPPDNMSLSVQFRNAGLFLVIFLWAWMGSETIWASERSDIY
                     LQSRSSAVLLVSVDRETKSLSFGTGFFMSQDGHILTNAHVLISSAKLLVYVPDQGVFT
                     DAKIVTIDQDADLAVLHLPQGHDRALSLFGTLAEDGAEGLAVGFPRVIDTLQMGLMLH
                     GTVKPVNVSGLTMGRSRTQGRTMPFLQVSGVLHAGTSGGPIVDAVSGQVLGIVVHSVP
                     YIGQATDRKGSLIGSVQLRADMSYAIPSTFIRQWLADQHIPYEMSPSQVASSSSSGSP
                     GRLPVDPQILGMGFFLTGHLMQTIAGTLNDEPEFMELAVNHYEKALEIIPGNMAILKN
                     MALGYGALHRYKDALHIYEGLLQQLPADPFILTETAKTLKLLHYDDRAMMMYRTVLDH
                     ESCHLDALNRLGSLYFSHQEYEKAVPTFRQAVKCEPSSAYAWFYLGESMTRSGLVEEA
                     HAAWKESLERVVIQNSQEKELFNLMRERAVSGPAILSHASPVEAISSPQVVSR"
     gene            40696..41085
                     /locus_tag="PKM72_00995"
     CDS             40696..41085
                     /locus_tag="PKM72_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59379.1"
                     /translation="MTIMRNSILTLACFGLLSTAAMGMPPESMNEPELHHGTNTDPTN
                     QPGPEHSESSMGSAQGNIMGSEITGEILTVEGDLYTVKDSTGKEVQLHVDESTEKSGK
                     LIEGDKIKAEVTPQGHAVMLKKANDMN"
     gene            41470..41616
                     /locus_tag="PKM72_01000"
     CDS             41470..41616
                     /locus_tag="PKM72_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59380.1"
                     /translation="MKPKAEALLLTGYFLFLISQTTASPAPLIVILKEAKDLCPVRRE
                     HGFV"
     gene            complement(41686..>43915)
                     /locus_tag="PKM72_01005"
     CDS             complement(41686..>43915)
                     /locus_tag="PKM72_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59381.1"
                     /translation="VNEELQSANEELQTSKEEMQSINEELGTVNAQLVSKVEELDQSH
                     ADVENLFLTSDIATLFLDSKLAIKHFTPAVQKIFGFRDIDLGRPVSELAAFLEDETLV
                     PDIQEVIRTSMVKEMERPLGKDSRIYLSRMIPFRTQDNLLEGVVLTFVDITRIRKAEI
                     QVADFSRRQNNVEVIAAFGRLALQERDLAKVMDECVRLLSRTLSVEMVKVLELLPGKQ
                     SLLLRAGVGWNDGIVGHAVVGAGRESQAGYALLSGDPVVVADLSQETRFSDPALLSNH
                     HVVSGMSCLIRDHEGVPHGVLGVHSTSKRSFTEEDVIFLQAMANILAYAVYRTGVEAQ
                     LQSIKDSLEGRVAERTDALLRHQHRIRNLASELLLTEQRERRRISNELHDYLAQLLVV
                     GKIKVSQLQQSGVSEQQSLLIKEMEESLDEALTYTRDLMAQISPPVLYEYGLLAALRW
                     LADKMGRYELHVTVTAHPDDESIHLPESAGVILYQVVRELLFNVVKHAKTGEARVEMT
                     RASPDIISIEVVDRGCGFDLDSTFEKIGKSPKFGLLNMQERLEGLGGQCEIYSKKEEG
                     TRVRITIPLYTRFMADPEADPEADPEADPEADPETHHHSEFSPTDVEKSGDLIRVILA
                     DDHPILREGLATLLNACPDIQVVGEAENGQKVIELVQMLHPDVVVMDINMPVMNGIEA
                     TRRIKASHPSIYIIGLSMHGDQIVMKNFSEAGGDEYVTKGDSFNSFAEVIRSSQKNYS
                     SS"
BASE COUNT        11577 a        11660 c         9896 g        10782 t
ORIGIN      
        1 acttccggat taagtgatga tcggaatccg tgataagcac atgtccttgt ggtgtgattg
       61 caataccata gggcctggcc aatgcctgag actgttcatt caccccccgc tcgtagcgaa
      121 actcggcgcc atcccctaca accgtggaca gaagccccgt gttgtggtcc agcattcgga
      181 tacggctatt aaacgtatca gcgatataga gatttccatc gtgatcgagt gccaacccgc
      241 tcggtcccgc cagcgcagat tccacggcag gcatgccatc cccggtatat ccggaatctc
      301 ccgtgcctgc gatagtcgtg atcacacctg atcccaaatc cagcatacgg atgcgattat
      361 tactctgatc ggcaatataa aggcgtgtac catccacgac taatgccacc ggttcattca
      421 acgaggcgga gatccccgga ccaccgtccc ccgtccactt cgactgaccg ttcccagcca
      481 cggtctggat aagacccgtc ttggcatcta cccgtcgaat gcgatggttc cacgtgtccg
      541 caataaagag ggtccctgcc gcatcaacgg caacggagga gggaaagttt aattgtgcac
      601 tcgtggccaa gcccccatct ccggcaaacc gatctttgcc aatccccttt ccaacgatgt
      661 aacgaaccgt tccactcaga tcaggcgttt gagagtaggc ttggccagga atctctgcca
      721 catccgcaag aggatcaatc tcttcctcta cctgagaaat tggagatggc atcggagttt
      781 ctggatttcc ctcaccggcg cggcgggtcc caacacaccc ggctaccgtt tcgataacac
      841 cagtcgaatg tatcagtcgg cgaatgacgt gattttcaga atcggcaatg aggaggttgc
      901 cttcagcgtc aaagcacaca tttttgggtt catttaaaac tgctcgggtc cctggttgtc
      961 cgtcaccctc atacccaggt tccccggtcc ccgctatcgt gataatttgc ccactcttcc
     1021 cttgcgcaat catcctttaa tgtcctaact gcttcaggat gtgattgccc atttcagcgg
     1081 ttccaaccag cgtactcccc tgactctcaa tatctttcgt gcgatatcct tgctccagag
     1141 tcctctgaat cgccgattca atcatctcgg cttctttcgt caactcgaac gaataagaca
     1201 acatcatggc tgccgaagca atggtcccaa taggattggc gagatttttt cctgcaatat
     1261 ccggagcgct tccatggata ggctcaaaca ttcccactgt cgctccgacg ctggcggaag
     1321 ggagcatgcc tatcgaccca gtcaacatcg ccgcctcatc actcaggata tccccaaaca
     1381 aattggttgt cacaaccacg tcgaattgct tgggatagcg aacgagttgc atggcacagt
     1441 tgtccacata catgtgtcgc aattccacat cggggtagct ctgatgcacg ttaatcactt
     1501 ctcgccgcca aagctcggat gactccagaa cattggcttt atcgatagag tgaacaaggc
     1561 cacgccgttt tttggctgct tgaaaagcca catcagcgat tcggcggatt tcctccgtcg
     1621 tgtagacttc agtattgaac ccccgatgtc ctgtcaccgt ttgttcaatg cctttcggtt
     1681 tgccaaagta aattccaccg gtcaattccc gaacaaccag aatatcaatg ccctcgacga
     1741 cttccggttt tagactggac gacccgatta acgctgaata catttttgcc gggcgaaggt
     1801 tagcgaaaag tccaagctgc tcccgcaacc cgagcaaggc gcgttccggg cgacgctcat
     1861 attcaaggcc ctcccacttc ggcccaccca cagcccccaa gaggacagca tcacacgatt
     1921 gagccaattt cagagtctcg gccggcaatg gaaagcctgt ggcgtcaatc gcctcccccc
     1981 caacctgccc ataggtaaag gtaaattgat gcccacattt ctgaccaatg gcctcaagaa
     2041 cccgaatcgc ttgtggcata atttccggtc cgattccatc acccggtaat acggcaatcc
     2101 gtttcttcat gattcccccg ataatataat gaacagtttt agattaattc cagcgataac
     2161 tgcggacctg aaatggtcta ccagaaacta cttgcccaac ggcaagacaa tggcaggaaa
     2221 tcaggtgctc atttaattgg atgaagggaa cgctttcttc aacacaaccc gattttttgt
     2281 tcggccttgg cctgtcgttc cgccaaatcc gccaatttgt tgagcgcgtt gaggtaggcc
     2341 tgcgcagaag caagaataat atcggtatcc gatccgtgac cggtaacagt ttcactattt
     2401 tcttcgacac ggaccgacac ttcaccctgc gcatcggtac cacccgtaat cgctttaaca
     2461 atgtaggcac gaagaagact tttcgtttca gtcaaggccg caatggtccg atacaccgca
     2521 tccacgggac catcacccgt acccgtccgc ttctcgatcc gcccatccat tatcaattcc
     2581 accattgcgg acgggcgctg ctccaaccca ctctctacat gcaacccctt taaggcatag
     2641 cgctcaggaa ctttggtaat agccttattg acgattgtct ccaaatcctc ctcaaataat
     2701 tccttctttt gatccgccag ccgtttaaag cgttcaaagg cctcttgcaa ttcttgatca
     2761 gaaagggtgt accccaggtt ggccagttcc tcacgaaagg catgtcgacc agacagttta
     2821 cccatcacca atcgactttg ggttaatccg acggattccg gtcgcatgat ctcataagta
     2881 cttttgtctt tgagcaaccc gtcctgatga attccggacg tatgcgcaaa agcattggca
     2941 cccacaatcg ctttgttggg ctgtaccacc atcccggtga tattgctcac caagcggctg
     3001 gctttggaga tttcctctgt gcgtattctg gtatcggact ggtaaaaatc tgttcgcgta
     3061 cgaagtccca tcgcaacttc ttctaatgag gcattgccgg ctcgttcacc aatcccattg
     3121 atcgtacatt caacctggcc tgctcccgca taaatggcgg ccatggaatt tgcgacagcc
     3181 aaccccaagt cgttatgaca atggacggaa ataatggctg tctccatccc ccggactcgc
     3241 tctcttaatg tccgaataat ctttccaaac tcctcaggag tcgtataccc caccgtatcc
     3301 ggaatgttca ccgttcccgc tccagcctcc actacggctt ccaccacctc acagagatag
     3361 gtcaaatcgg aacgggtggc atccatggga gaatattcca catcctcaac gtacccccga
     3421 gcatggcgaa ccatctccac cgctctcttg agggcctcat ccctggtcat acgaaattga
     3481 tgtttcaggt ggatatccga agaagacaaa aacgtatgga tacggcattt gggtgcacct
     3541 ttgagtgcct cccaagcccg gtcaatatct tccggtttcg cccgggccag actgcaaatg
     3601 accggacctt ccacttcctg ggcaatacga tgaatcgctt caaaatcacc aggagaacta
     3661 aacgcaaaac cggcttcgat aatatccaca ttcaacctgg ccaattgctt cgccacgagg
     3721 attttttctt cgatattcat gctcgcgcca ggcgattgct ctccatcccg caaggtggta
     3781 tcgaaaattc gaatcatccg tgtcatggct catttccccc acatttgctc ttcatccaag
     3841 ctccaatttt ttcccgcctg ccggtcattt atttgtggag tccatccgtc cccacttctt
     3901 ccgtaggctt tttccccatc agacctgcca caagcttcca cccttgctcc aagacgcctg
     3961 aaagcgcata ggcagcaaac acaacaaaaa gcatcgcctg tggatacgct aagaccgcca
     4021 tgagaatgag gactgcatac accaaataat taaaatggtg gtgttcctgc gtttttaatt
     4081 ctttcagact acgataacga aaggtgctca ccatgagaaa cgccagccct aaggtcaaga
     4141 cgatcccaag aataggcttc accttctccc ctaaaggcgc aacatgatga tcgaaaatca
     4201 ccaacgtggc catcaccccc gctgcgccgg gaataggaag ccccgtaaac ggcttaccat
     4261 ctccgccagt tgtcgccaaa acgttatgcc tggccaatcg caaggcacca caggcaacaa
     4321 aggcaaacat caccgcggca cccaacatct tcggaccact gagcgcccag gaatacatca
     4381 gcaagcccgg agccactccg aaagccacga catctgccaa cgaatcgtat tgcagcccga
     4441 aatcactcgt acttttcatc agacgggcgg acgtcccatc caacacgtca aaaacgatcg
     4501 ccacaagaat cgcaatggcc gcaggcccat aatcgccccc aaagacaaac aagatagagg
     4561 ccagaccact aaaaagattt ccggtggtca gaagattggg aattaaatag acgacccgtc
     4621 gttttttggg ttgtttcatg aaggctccag gcatggcaac tccgccacga tggacgatcc
     4681 ccccttcact ttttcgccaa ccttgacttg aatggcacta tcgtggggaa gatacaaatc
     4741 catccgagat ccaaacctta taagtccaaa ccgttcgcca acttccacct gttcacctgg
     4801 cttcacccaa caaacgattc gcctggcaat taacccggca atttgcacac acagtatttt
     4861 tttcccatcg gaacgcttga gcatgagcgc attttgctca ttttccactg tagcctctgg
     4921 acgactggct actaaaaact tacctggagc atagacgaca tcctgcagca ctcctgtcgc
     4981 aggcaaccgg ttaatatgta cattgaagac atttaaaaaa atactgattc tgatactggg
     5041 ttctttgaga aatcgatgtt caaattcctg ttccaccgct acgaccgtcc catcaccggg
     5101 agacactatg agatttctcc cctgcggtat cgttctggcc gggtttctaa aaaaccaggc
     5161 agtaaacagg gttaacgatc ccatcaggca cgtgagccag accggcccgg ccacccaaca
     5221 cccaagcgtc aggagccctc ccccagcgat aaagggtagg ccttctttgg ccacaggaat
     5281 cccttttgcc tgatcagcca ttaggtatgg gtctcacgtc ccgcaacctt ctcatcaatt
     5341 tttctgttta tccacaagct gatctttttg cagccaaggc atcatcccgc gcaaccgtga
     5401 accgacttct tcgatggggt gctcctctcc ccttctcaaa agggcattat acacgggccg
     5461 gttcgcttga ttttccaaca cccactctct ggcgaatcga ccgctttgga tctctcccaa
     5521 aatctctttc atcactttct tcgtctcatc ggttaccaca cgtggtcctc ttgtcacatc
     5581 gccatatttg gcggtcgtgc taatagaata gcgcatattg gcaatgccac cctgatagat
     5641 gagatccact atgagcttca cttcatgtaa acactcgaaa taggccattt caggcgaata
     5701 accggcttcg accaaggttt caaatcctgc ctgaatcagg gaggtaagcc ctccgcataa
     5761 gactgcctgc tccccaaaaa gatccgtttc cgtctcttct cgaaaggatg tttcgatgac
     5821 acccgctctg gctccgccaa tcgcacaggc ataggccaat ccgaccttgg ttgtctgacc
     5881 gccgggatcc tggtgaatgg ctaacaagca tggtacgccc gtccccttgg tatattccga
     5941 cctgaccaga tgccctggac ccttgggagc caccataaac acattcacgt ggctggcagg
     6001 ttgaatttgc ccaaaatgga tattaaaccc atgcccaaag gccagataag ctccctgttt
     6061 caagtttgga ccaatatctt tattgtaaat ggcggcttgg gcttcatcag gagctaacag
     6121 catcaccaca tcggcacctt tcaccgcatc tgcagtcggc atgactttca acccggccgc
     6181 ttccgccttg ttccatgacg aaccttcccg gcagcccacg accacttgaa gaccactatc
     6241 ccgcatattt aaggcatgag catgaccttg gctaccgaac cccactacgg ccacagtttt
     6301 ttgggctaaa atctgtcgat ctgcgtcttt ttcataataa attttcatca tgcactcctc
     6361 ataaagataa aatagtcctt agtcgttccg tcgatcaggg tggacggatc ccctcagtct
     6421 aaaggcaggt gttggttggc ctgattaatt cgctttcgca agcggtcggg attgggtttg
     6481 tgtcgagcgt gtcggttcgc gcccaatggc aattctgccg gttcgtacca gctctttgat
     6541 gccaagtggc tgcaggagat tgatgatcgc ctggactttc ctcacatctc cagtgacttc
     6601 aatagtgtag ctggtcggag aggaatccag aacattagct cgaaaaatat ccgcaatgcg
     6661 aagagcttcc gctcgatcct ctgcccgggt atgaacctta atgagcgcag tctctcgttc
     6721 aacaaactcg ctttcgctaa tatcaacgac ttttatcaca tcaatgagtt tgttcagctg
     6781 cttcaggatt tgttcgataa tcggatcatc ccccttagtc accactgtca tcatggacac
     6841 agagggatcc agagtggggg ctactgacaa actttcaata ttaaaacccc gcccgctgaa
     6901 gaggcccgct attcgagaca atgatccgaa tttattctct acggtcaagg aaataatgtg
     6961 ctccataata cccttttata gttgtactct aagctgtgat gacagaatct gaatccgccc
     7021 gagtcccgat tttttgcatc gcattgggtt tcttcaagtc cgggggatcc cctaacaaca
     7081 tttcatgatt acagcctccg gccgggatca tgggataaca attttcaaac tggtaggtcg
     7141 ggacatcaat cacgactggc cccttaatgg agagggcttc acgaatgccg ctatccatct
     7201 ccgacacctt ttcgatccgg ataccggtag ccccataggc ttcagctaac ttaacaaaat
     7261 caggaacggt ccccaggtaa cttgaggcat accggccttc ataaaacaaa tcttgccact
     7321 ggcgaaccat gccatggaac cggttattaa taataaacac tttaactggt agttgctcga
     7381 cgaccgcagt cgccaattcc tgggtattca tttgaatact gccatcccca gcgacgcaca
     7441 aaaccagacg atccgggaac gcagcctgcg cacccatagc ggccggcaac ccaaaaccca
     7501 tagttccaag accaccggat gttaaccagg actgaggatt ttgcaattta aaatattgcg
     7561 ctgcccacat ttggtgttga ccgacatccg tggccaaaat aggatttcga tcgctcgtta
     7621 agtgatacag acgatcaata agaaattgtg gcttaattaa tccgtcagga tcttggtcat
     7681 agtgcagtgg gtgtgcttcc ctccaggcat tcagttgatc ccaccagggc ttccgttgtt
     7741 ctttttgatt cccattcaca gtggcacgaa gaatattatt caattcggtg agcacccgct
     7801 tacagtcacc cacaattgga atgtctacct gaatattttt tctgatggag gtcggatcaa
     7861 tatcaatatg aataatagtc gcatcgggac aaaactctga caccttgccc gtcactcgat
     7921 catcaaatcg tgccccaacg gcaataacca ggtcagaata atgcatagcc atgttcgcga
     7981 catacgttcc atgcatgccc aacattccca gggacaatgg gtgattgcca ggaaatgctc
     8041 ccaaccccat caacgtcata tccaccggga tttgagtcaa ttctgccaga tccttcacct
     8101 catgagaagc tccagacaac accactccgc cacccacata taaaatagga cgcttggatt
     8161 tcataatcgc gtcagctgct tgcttaattt tccaacgact tccgtcgtag gtgggattat
     8221 atcctcgaat ggagacagac gtcgggtagt gaaattccgc tttgtccagc gaaatatcct
     8281 taggaatatc gactaaaacc gggccaggac gaccagtggt ggcaatataa aacgcttcct
     8341 taatcgtctg cgctaaatcg ttcacgtcct tgactagaaa attatatttg gtgcatggac
     8401 gactgagacc aatattgtct gcctcttgaa aggcatcatt gcctatcaat gcggtcggaa
     8461 cttgtccaga aaaaacgacg atgggaactg aatccatata cgcatctgca agagacgtga
     8521 taacatttgt cattccgggg cccgaggtaa tgagcgccac accgggtttg cccatggctt
     8581 tcgcgtatcc aacggccata aacccggcac cttgttcatg ccgagtcagg attacccgaa
     8641 tatctttttg ctggtgcaag acatcgaata ttttgagaac cactccgcct ggtaaggcaa
     8701 acatggtttt cacgccttca cgctttaatg cttctaaaaa aatttctgca ccagtgattt
     8761 tcatgattct caccctctgg ttaatttgac gttggacgag acctggtgaa ccacatcgtc
     8821 acgggaaatt ccctcaaaaa cctctccaac aaaaaagatt ccccaattgt gataatttta
     8881 ttgtctgggg aagtaaataa accatggtaa tcattatggt tttttcaagt caacatttat
     8941 tgtaacaggg ttgatcgcat tacgaaatac ctgtggatcc cgggctccct agcttagaat
     9001 tccaaaattc tgtgggcatc tcaatgttcc aagaagagct tcagacccga gcgcaacaac
     9061 acctcctgag ggaattatct ctcatcaact ccccctcgaa tccggttatt tcagtggaag
     9121 ggcgggaagt cctccttttt gcctccaata actatcttgg actggccaac catccaaagg
     9181 taaaagaggc cgccgtgaat gccatcgaaa aatttggggt aggatctgga gcctctagat
     9241 taatttccgg caccatcact cctcatcacg tattagaaag ggaattgggc agttttaaaa
     9301 acgcagaagc ggctctcacc ttttcttcag gatacgccac caatatagga attatcccga
     9361 atcttgccac ggcggacagc ctgattttgg cggatcgact ctgccatgcc agcctgattg
     9421 atggttgccg tctcagccgg gccacattgc gcgtcttcca ccataatgac gttggccatc
     9481 tcaagcgcct tctgtccgga ccacgttccc gaacctcgac gctcattcta actgaagggg
     9541 tgtttagtat ggatggagat atggctccgc tgcctgagat ggttcgattg gcagaagagt
     9601 atggtgccac cctgctcatt gatgacgcac atggtacagg ggtcatgggc gcaaccggtc
     9661 gaggcacggt ggaacatttt gggttagacc cccgccgcat cctgcagatg ggtaccctca
     9721 gtaaagctct tgggggtagt ggcggatttg tcgccgggcc gaaagatttc gtggcctatc
     9781 tggtcaatac cgcacgatca tttatctata gcactgcccc gccaccagcc atcgccgcag
     9841 cagcgagcgc agccctgtca gtcattcaag aagaacccca aagaagggca aaactctggt
     9901 gcaatcgaga gtttttgcat cacggcttag tagccatggg atttgaactg accaacaccc
     9961 agagccctat tatccctatc atggtgaacg acccggaact cggagtccaa atgagccaac
    10021 atctacgagc agaaggagtg tggattcctg ctgtccgccc ccctacggtg cctaaaggga
    10081 ccagccgcct cagaatcacg gtcacagctg accattcgcc agagcatctc gaaactgccc
    10141 tccgggcatt acggaaagtg ggaacagcct ttaaggttct ctgagaaaag aattgctgcc
    10201 cttgagcttc tgttgtttct gccagctgat cctaaataac acctttctct gctgacctac
    10261 atccgaaact ccaagcgatc aagaattccc tccattcaag aaaatcaaac ggtattccga
    10321 caagatgagc aggaattcca tgggtccgag atttcttcat tattattgat aagtgtgtct
    10381 tcgtttcaat gggccacgat caccatcacg tcttcatttc tcacaaaggt tcattgaaac
    10441 ggttaggttt attcatatga atacacaact gttgcccgga aaaattgaaa tacaaggaga
    10501 aatagccaaa ggccaaaccg ggaccattta ctatgggtat gatctcgaac ttcggcagga
    10561 aatggcgatt aaggtttatc atacccatat caatggtcgg ctcattcctg gaagagcctt
    10621 catcgaaaaa gcccaatcgc tccttcgatt ggagcatccc aatctcatta aaatcttcaa
    10681 agtggaagag caggacggca cccctatcgt ctgcatggaa ttctttgacg ctcccagtct
    10741 ccaacacgtc attcagagtg aggggcctct ttcagtcgaa aagatgctca tgctggctcg
    10801 tgaaattgcg gaagtcttag ttcatatcca ctttcaaggc atcattcacg gcacgctcca
    10861 tccagggcat gtcctggtcg gccctcaagg ccagatcaaa gtcatggact tcggattatc
    10921 ctggatcctg atggacatcc tgaaaaactg tgacgctgag ttactcagac cattacccta
    10981 cttgctcccg gaaactgcca aaagcgaact cctgaccctc ggtagcgatt tgtactgttt
    11041 gggattcatg atgtacgaca tgctcaccac gcgagtgccc tattcaggtc tcccaaaaac
    11101 atcgattatg ggaaaactgg ctttcgacca ggctgaccca gtgtttgact tcccggacca
    11161 tgtcccaaaa gccatctgcg agcttatccg ccaaatgacc cggaatcagt cctcacagcg
    11221 acttcaagat gccacccatg ttctcacgat tttgaatcag caattggcaa aatttgcccc
    11281 tgacgttttg aggcccccag tggcccctgc agaacctcaa ccaacgccag aaatccaaac
    11341 aaccaaggta gaggcaccga gccttccacc acaggcacca gtatcagtac cctcttcaaa
    11401 ccaaccgcct gccgtgaccc ggccgaaaca ctctaccgac tatcaaggaa tacaccaagc
    11461 caagacgaga aaaaaattag gcgtaacgat tggaatcagc ttgttccttt tgcttggtgg
    11521 caccatgggt tatatctaca aggaaaggct cggcatcccg tttcttacat cccctgaatt
    11581 cagggtccca gagcagccac gaatcaacga accggcccct tcagttcctc caattgactc
    11641 gttcacgacc atgacccctg aacccccaat ttctcctcca ccccattcct cacaggcaga
    11701 ggagccgcgc ctaaatacca atgacccatc aaagggcgat ccctcactca ggaccacacc
    11761 accaccaatg ccgacacccg tggtgacaaa ggatcaccca ttgcaacctg atgtatcaca
    11821 acacccagga acgccattgc cttccacact ggaacgcaca aggacacaac ctcagaactt
    11881 gagcccgcaa cccaaacccg aagccaagcc agttccggct caacctcact cgacgccacc
    11941 tcaagacaat tccacgattg gcaatccacc attgctcaag aaaccgacaa ctccggtcac
    12001 cctacccgcg cccacaccaa agcctccagc aatcgtgtcg gaaactgatc aagcgggtgt
    12061 ccccccagac cctgataaac attcttcaca ctccaaagag tcgtcagagg tttcccacac
    12121 gggaccccaa cccatacagg actcgactgt tctctccccg aacgcagaat cgctagacaa
    12181 cattgaaagc aaggagctta aagacatatt gaattcggtg cagagccctg agaccgtgcc
    12241 catactttca aaagatgggc caggtacttt acttccatcc gctccaaatc caccgcccct
    12301 ttccccttca ccgaaactcc cggctctttc gtcaccagta tctcccccat aaacacaatc
    12361 tcacatgacg gattctctgt acctccatga acgctaccta taatccttga ataaacagac
    12421 cctacaggtt gtaatctaaa tatatgaaac aggcatgtta gccaaaattc aaagtgtggg
    12481 attggtggga ttagacgcta atctcattga aattgaggtg gatataggcg gtggcctccc
    12541 acaattttcc attgtggggc tacccgatgc gaccgttcgt gaaagccggg accgggttcg
    12601 atccgcactc aaaaataccg ggttccactt tccagcaaaa aaaatcaccg ttaatttggc
    12661 cccagccgga ttgaaaaaag aaggagcagg acttgagttg ggaattgcca ttggcatttt
    12721 ggtagctgaa ggcgtcctgt cccaggaaga ggtcatgtcc tacatttttg tcggtgaact
    12781 ggcgctggat ggcagaatca aagccgttcc cggagcgctg tccatggcca ttgccaccca
    12841 tcaccccttt tcattaattc taccgcacga caatgcctcg caggctgccg tggtcgacca
    12901 tgccaccgtg tttggcgtct ccaccctccc tcaggtggtg gaatttcttc aggggtccct
    12961 caccttcctt cctgcaaaaa ataccttctc tattgggcaa gccggcccct cctctatcga
    13021 ggaagacttt gccgatgtgg tgggacaata tcaggccaaa cgtgccctgg aggtggctgc
    13081 ggcaggcggg cacaatcttc tgatggtggg accaccagga tcaggaaaaa ccatgttagc
    13141 ccaacggtta acaggtattc ttccacccat gagctttcag gaatgtttgg aagccagtca
    13201 ggtccacagt gtagcgggaa accttccacc tcatgcggcc cttcttgcca atcgcccctt
    13261 tcgggctccc catcacacca tttcagaagc tggcctcgtt ggaggaggat ccatcccccg
    13321 tcccggagaa gtctctctgg cacatcatgg cgtcctcttt ttagatgagg ccttggaatt
    13381 taagcggtcg ctcttggata gcttgcgcca gccactggaa aatggaaccg tcacactgac
    13441 ccgcgcccat gccagcctaa cctttccagc cagactcatg ttgatcgtcg cgatgaatcc
    13501 ctgcccatgc gggtattgcg gagaccccac ccatgaatgc gtgtgtacgc cctatcacat
    13561 tcaacggtat cgaagccgtc tgtccggtcc actccttgat cgactggata tccacatcga
    13621 agttcctgcc gtcccgatga aagaactatc gggtaggaca atcggtgaat cctcttcgaa
    13681 aattcgagac agggtgatcc gggcacgaca ttgtcaaatc gagcgctaca gtgcggaaca
    13741 gaccttaaat aatgcacagc ttaagcctcg cttgcttaaa aaatattgcc aattggatca
    13801 agccggaaac acactgttgg atcaagccgt cactcgcctg ggactatctg cgcgggcgta
    13861 tggccggatt ttacgtgtgg cccgaactat tgccgactta ggagaatcgg ttatgatttc
    13921 atccacacat gttgctgaag cgattcagta ccgaacatta gatcgaccca ttctggcata
    13981 attggcataa ggacatttca tggaatcgtt taaagttttt cgatggtggt tcattattgg
    14041 ttctctcatg gccctggctg tcattatgat tcaagggggg gtccgcgatc tgatgttagc
    14101 gaatgaaccc atatgggaaa tcaagctggt cgagttgggc cccccgatct ttgggggagg
    14161 tctattgggt gggtgcattg ccctcattct gaatcgaatc aaagataagt cctgactcag
    14221 ggaaccattg acggcctttc cgtctaattc cccctatcgc atcccaaggt ccctcacaga
    14281 ctcattccaa ccccttcctg aatttccaat cctctatctg cagttgcagc acagcaagag
    14341 tcaaatgggc aatttttgga tggttgcaca aatccctggg gctgatgcca atttttaata
    14401 ttttgtcttt tcaacaacac acgtttacca gaaacccgac ggggaactag accatggcca
    14461 agcagccaaa acacattcct aaaagcctca cgaagagtgc aacctttgct ttcaacgctc
    14521 ctccggaaca tttcctctag ccaccaggct gatccggtca gtacaatacg attcagtatt
    14581 tagtctattc actgacgaaa aaaggaggct tttgggcatg gatgtgaaag tcggaccgca
    14641 ctggtaccgc aattccaatg gaattgttga agtcgacgga ctccctcaaa tcgaaatgac
    14701 actccgacaa accggaaaaa cccctgtccg agtaaacttt gccatattcg ataaagttgg
    14761 gaaaattcac gcaaagatag aagccaattc tctggtcatc aacgaacagg gagcctatca
    14821 cctggaacgg ttggagaccg gcttggtttt atccaacaga ggggatcaaa aacgcgtgct
    14881 ggccatcaac gtgacgggca ctgatcaggt ggaaataccg gaaggcgagt tctatacgct
    14941 caaaggtcat ctcttaaaga ttacccccaa tgaatggtca gtggaaaaaa ccacagaaag
    15001 gtcaggcgag accgacatga agggccaagc agtcgctgtt ggctctcagt aaatgagcta
    15061 aggaatcgtg ggttcgttga ggccaatcgc ttgacttccc catacaatca cggtctttaa
    15121 atttcagacg acggcgcatg ccaccgccct ttacaaattg cacagacttg cccgcggacg
    15181 ccgtctccga caatggcctg gcctagtgaa atacagaggt ccacaacgcc acgacatctg
    15241 acccctgata cgtccacaaa cggccacatt cctgacaaca atactaaagg gtatatccag
    15301 gccactctcg atcacgcaca gccatgtggt tggcctcctt tgccaacctc ttacttacac
    15361 aacccaatga ttcggactca atcgtctggc atttgcgccc ggactttttc aggcaacgtc
    15421 tgtacccact catcaaaggg caggtcggca tccacgacaa tttccaactt accattgggc
    15481 aaccgacgaa ccactccagg acttgccggg gagagaggga tttccaccgc ctctcgtgaa
    15541 atatccaaat catctatcac ttctaaaatc ctcgtgatgt gttgaaacga aaccgtatcc
    15601 aataaactca cgtcgactcc cttcgccatg ctgatcaatg aaattgtaga aacggtgcca
    15661 ggctctgtcc aagaactcat agagagtgga aattcactcc gtggctgaaa aaacacgcct
    15721 gaagatccga cccgcggcga ccatgtcttc atgattaaca tccaaatgcg tgaccacccg
    15781 aaacgcccga tctcccatgg cattcaacag gactccggcc tccaggcaat cagctaataa
    15841 cacctcggtc gttttcaatg accgagggac ctgaaacatc accatattgg tctccacagc
    15901 cttaacatcc accaccacgc cgggaatgtc ctccaacaga gtcgccaggt aatgcgcatt
    15961 cgcatgatcc tcagccaatc gggcaatatg gttctcaagt gcatacacac cggccgcagc
    16021 cagaattccc acctgccgca ttcctccgcc aaacactttg cgaaggcggc gcaatgtttg
    16081 aactcgtgcc gtatccgaca ccaccagcga cccaatggga gcccccaaac ctttcgacaa
    16141 gcaaaaggac accgtatcga aaggacgaga atagtcagcc gccgccaccc cggttgcgac
    16201 cactgcatta aacaatcggg caccatccaa atgcagggcc aggccatggg tacgggccac
    16261 ctctgcaatt cggttgatgg tctccaatga ataaatcgat cctccaccca cattatgtgt
    16321 ctgttcaaga cacaccaagg tggatggagg attatgaaga ccctttggcc gaatggccgc
    16381 cttgaccaat tccggcgaca ggcgtccacg atcacccggc acacaacaca attggacacc
    16441 ggagagtgaa gacgccgatc caccttcata gcgaatgaga tgggatgtac tgtcaacgat
    16501 cacttcatca ccaggccgcg tatgtaaccg gagggccaat tgattgccca tcacccctga
    16561 tggaacatac actgcggcct ccttgcctaa catatccgca gccatggctt ccaagcgatt
    16621 cacggtcgga tcttctccat acacatcatc gcccacctca gcctgagcca tcgcttctcg
    16681 catggccggt gtcggtttcg tcacggtgtc gctacgtaaa tcaatttttt gcataaatca
    16741 attccttcaa caaggatata ttctattcgt attctaagga accgtcctcc atcacacaag
    16801 attttccttt tggcattggt ctacaatgcg gacgatgaaa ccacgtctcc tcgtcaccgg
    16861 cggagcgggc tttttaggga atcacctcct tcgtcgggcc aatcaatttg tcgcagccgg
    16921 aacgctgcac gccacgccct ccacatccct tcccggcgta acctttcatg tgtgtgatct
    16981 ccaaagcccc gaggaagttc gagtcctcat ggatcgggtg caaccggacg tcattattca
    17041 caccgcctgc tcggaccagg gaaaaggaat tgaagcgatc ctccctgcag caggactcct
    17101 tgccatgcaa tcagtagaac gaaacattcg tttcattcac ctttcgactg atcaagtctt
    17161 tgatgggact tccgcgccct ataccgaaga aagccccacg aaccccatca acccctacgg
    17221 taaggccaaa gcccaggcgg aagaactcat ccgctccctg aatccgaagg ccaccatcgt
    17281 ccgcacctcg ttgctctatg atttgcgcac accggaccgc cagacgacac gcttaatcga
    17341 atccacaaaa accggcgaac agtaccgctt gttcatagat gaacttcgct gtcccatctg
    17401 ggtggaaaat cttgccgaac tgttactgga agtcgccacc aagaatatcc cgggaatctt
    17461 acatctaggc ggcccagata gcctaaaccg gtgggaattg gggatgggat tgctcaggca
    17521 ctttggagtc acacagacac tgaatattca aaagggaaca atcgaggaat ccggattgtg
    17581 tcgccccaaa aacctcacca tggactcctc ccgatccgaa cggctcctca aaaaaccgag
    17641 ggtttctcta caagaagccc gcaagatgac cgtcacctct gaaaggtaaa attcttttaa
    17701 cacacctttt ttaggattac agaagactcc taaaaacatg gtcctaccta ccacctgcta
    17761 gtccaggttc ttttttttcg ggccctgccc cctgcaatat catatgcctc acgacctgca
    17821 aatcggcaaa caaagacagg ccttgtctgt tctggacaag cctggccgtt cttcgcaaat
    17881 cgagatagat tcagccgaca gaattcgtgg aagtcgatct caagggatgt caccctggca
    17941 ctatgaggag acgctgcgga tgcaacaatc ccaggacaca aatttggcgg ttatccggcc
    18001 tttgaaaatg gggaagcgag ggtaagaaca cacccttttg atcaattcaa aaaaaattaa
    18061 acgagcgtcg gtaaagaaca gcacggttgg cggaagaccg cggaaggagt ttaagggcct
    18121 gaacaaaagc ttccagcact gcgatcttct gatcggcgac acctgaaatt gaccgaatcc
    18181 ggcctcagag tgcgagctta tcctatccga agtaggggca gcatgcgctc tgaggtctat
    18241 ttaaatgggt catgggaaat tgattcaaac gaaacttgga gctgttcgcg aagacggaaa
    18301 agaattactc cacattgtgt ttaatttgtt ctctgcttct tcatctggtt catcaatggc
    18361 agagtatttc acaaccatga ccgtcttcag ttatttctca ttcacacaac tgtcttggtt
    18421 cttttctatc gattgttttt gacgtccttc cattccttac ggaattcatc cattccggca
    18481 tggactgcct ctcttatcgt ctcctgaatc tctgccaatc ccttatgcgt gccggacacg
    18541 atgtcttgat aggtctcaac agttttttct tgcaaatcac tccaatcttc tccttttcga
    18601 cgaacataat cacgaatttg ctcccgcgag tcacgtcccg actgaggaga aagtagtaat
    18661 gtggcaacag atccaataat taagcccatg gcaaatacac ctaacatctt tccatctacg
    18721 acatctgatt catggtgacg catacatttc ctcacttcta gtgtttagtt tcgattaacg
    18781 atttccataa tgtataagta ccatgaagac gaaccatggt gaatagacaa atcactacga
    18841 gggatcatca aaaagtagtc cacaacgatg tctcagaaat taggaaatgt gatgatgcac
    18901 catagtaatc gtgtgacgaa ggtattattt gtagcagagg atgtttcaga tcatattacc
    18961 gaccatcatg tttccctccg gaagaagaac cgggattttg acaactatgc cacatgagcg
    19021 gagccaacag gaatgacggt taggtctggg cgtcccgata tttcaaataa ccttcacaac
    19081 catcatggcc acaccgacat ttccctaccg gcgcggtaat gacattcgca cattcttctg
    19141 aacagtattc ctcgccttct aaattttcgc atgaacatgg gggatgggca cagacggtat
    19201 tcatccggaa aacctcactt tcttccagaa aacggagtat atgacgctaa aatatcaatg
    19261 aaccctttag agtttcatag ccggactctc cggcatttat ccacttttcc tgactctcga
    19321 aaaacatttc ttgacaatta tgtctgtaat ttttcctctt tagcaacctt tctgaccgtg
    19381 gttgttattc cctctgggga tataagctgc ctcgatgcca tcagctctga caaagaatgc
    19441 agaagattcc ccgcttgttc cagacatcga aacgtaaccg acatttccgt tggtattgtg
    19501 gcccccaata ttcggattcc ctgcatccaa gagatacccg tttccttcat taaggacatc
    19561 gtttcatgtc aatggcttgt ctgaacaagt gtttttgtct cggcattgga ccacctggaa
    19621 agaacgctcg ggaatttgtc caatgcttac ggatggaggt tcagccatta ttctcactca
    19681 gaaggagttg ccataatcaa tttccttaat gtgtgtttat ttcaacttgt gcgctttaca
    19741 accactctca agcacgaaaa ggagttcatt atgaacaagc agcttattac gggtgtaatg
    19801 gccatcgctt tcactctatc ggttaaccca gcctgggcgt tgttcggaga tgataaggca
    19861 gaattacttc agggcaccaa agttacctta gtcgaggccg tccaaaccgc tctaaccaat
    19921 gtaaagggaa aggcatttaa tgcagagctg gaaaaggaag acggcaaaac ggtattcgag
    19981 gttaaagtca tcgatgagac aggagccgca agagaaatct acgtggatgc ccaaacagga
    20041 gaggttctca aaattgaaaa ggactaaacc tggtcctggc ttaaatctga cattggaagt
    20101 cattccctat ggactgatgg acttggaatg gcaaaacagg cttgggtggt aaagataaag
    20161 taacttgaat ctttccgttc atcttcttat cacacccgtg aacatgtata taaaaaatgg
    20221 aggaatattc aggctacgaa ttcaatgacc attggcctgc ccaataatta atgagccgca
    20281 tcgcaggcat atatccggcc ttgcgagcgg ctcatggttt tttactggct aagccccgtg
    20341 aataatcgaa agttatctcc gccaggaaat ccagcaacaa aaccgacatc ctgcgaccct
    20401 cccccccacg gacctcagac ccttacgagc catccttagg gtttgaccat cgactcattc
    20461 gggttgaaca tgctgacgta ccaggcttag aatgttgatt ctgaccattt ttaaccggca
    20521 cgtgaaggat cacgacctga ttcggttgtt cctcttcata tttttgagat tttcttatcg
    20581 tcgtgaagca atgatccttc tcagggtctt ttccccattt acatgaaaga cattcaaaca
    20641 cacgaccggc accggcttgc agaaaaaata cgatttcttg aacgagacgt cattgaggtc
    20701 atcacgacca catttctcca acaacatccc gagaccaaag aactgggaga agttgccaaa
    20761 aaccactgta tcaccgatgc acgctatcac ttgacctttc tcagaacggc cattgaattt
    20821 ggaaacaagg acacattcag acaatatctg cgttggacga caaaggtgct ccagacaaga
    20881 aacattccgc aacatgtgtt acagcgattt ttggaacaca tttctcatgc attggaaccc
    20941 cacctgaccg agcccgaaaa aacagtcgtt cggtcccatt tgttggagac cccagaatct
    21001 gaaccaccag aacctcaaac ttccgctcgt tcaacaactc ccttagcact ccatcaggag
    21061 gttttccttc aagctctttt ggccggtgat cgccatgcgg ccagaacgat cgcgctagag
    21121 gccttttcca atacccgctc tctctcggat gtgtatgtgg agctctttca agaatcgttg
    21181 tataagatag gcagcctttg ggaagccaat cacatttccg tcgctcaaga acacgtggcc
    21241 accgccatca cccaatatgt catggccaat ttatatacgt gtgtgacacc tgcccaaccc
    21301 tcgcaaggga aagggatcgt caccggcgtg gaaggtgaac tgcatcaaat cggaccacac
    21361 atggtcgcgg acatgctgga aatgcaggga tgggacattc gatttttagg cgtgaacgtt
    21421 cctcatcaat ctatcctcca attgattaag gatttccgac cgacactcgt cggaatttcg
    21481 gcaaccatgg ttttaaaagt tccgaccgtg agagatttga tcaaggacat tcgaaaccat
    21541 ttcccctccg ctgccactcc ccgcatcctg gttgggggat cagcctttcg tgcacttccg
    21601 gatgtgtatc gggatatagg cgccgatgcg tttgcgtatg atttacggtc cttaacggca
    21661 ctattacctt aatggacaca tatcaatggt tttagtcata tcacgattta aggtggcaaa
    21721 cggacttgaa ggggacgtgg tcgaagcctt tcaaaagcgc ccgcgattgg ttgaggccgt
    21781 tccgggcttt ttagggatgg aagtctacac gcctcactca gatcaaacaa ttttccattt
    21841 ggtgactcgt tggacggatc gggaaacctt tcacgcctgg cataaaagcc cggaccatcg
    21901 cctttctcac ggcatgcttc ccacggggct taagctggac cctaccgtga cccaaatcct
    21961 ggaactggag cgtctcccgc aaacggggct acagggcgga ctacaggatt ttcttggcga
    22021 ctcgatcctc ctcctggaaa aattcctcaa ccagacagat accattgtgt attgcgtcat
    22081 gacgcctgat ggacggattc aggtctacaa tgacgtcctc ttgaccaaac tcaatgtgtc
    22141 cagagcggaa ttgcagacaa aattcatctg ggaatttctc actgatccgg atcaaaagca
    22201 cctgcgcgat atccttgaag atgggatctc cagccacagc aaccggatgc gacttaattt
    22261 cctcgattcg tccggaatcc ctcacacact ggactgtttg gtgcagaaga attctgctga
    22321 caccaccatt ctcatcgggg aagaaccgga aaagttcaac cagagggcgt tcgtggaact
    22381 catgcagatt caaaacgaaa tgacggttaa aatgcgagag gaagtaaaaa gcgggaacaa
    22441 actgtcagaa aataattcat atttgaatca gctggtgacc gaacggacaa aagacctcgt
    22501 ttaccatcag gaacaattgc gtgccatgtt ggtgaagtta gcccttgtcg aacaatccga
    22561 acgccaaaat ctggcaaatg atttacacga ttatctcgcc caaatgctca tcgcctgcag
    22621 actcaagctc gggcaaatag accatgagca cactcttccg tcacattcca aggtgattca
    22681 ggaggttgac ggaatgatcg atcaatcatt gacgttcacc cgaacactga tctctgattt
    22741 aaacccctcc atactccagc accatggttt actcactgcc ctcaaatatc tttccgaaga
    22801 catgcagcgc tttggtttaa ccgtggcaag ccctgagacc atcctaccgg tgaaggtggc
    22861 ggaagacatc agcatcgtgg tgtataaaac ggtgcgagag ttactgttca acgtgttaaa
    22921 acatgcagga gttaccaccg catcgataca ttgggaatgc ccttcggaac atgttctcct
    22981 gataagggtg agtgactccg gaaaggggtt tgatccagaa acccggcaac ttgaacaaga
    23041 acggggacat cttggtctct tcttttaccg ggaacgcata agggcgttaa agggacaatt
    23101 tgatattaca tccagcgcga aaatgggaac gaccattacc atcatcgttc ctccatcccc
    23161 gtaagtcctt tgctcgtctt atcatggcct tcccacggct tgccggactc ctctgaaaac
    23221 cgtcacaata ggttcagacg cctttaggtc gacatagaag cttccttttc aagggttttg
    23281 gtcagttggc cataggccgg actctcgtta cagtgaagaa atgccaggca tccgaaccta
    23341 acatggaagg aacatcatat tgcaccactt ttatgtagac actcctacaa aaccatatgg
    23401 tgtaattttt tcccttagac atgcggattg gaatgtgcaa acaaccattg atatgtgaca
    23461 tttttttaat atatgaacac actcaagcga gcaagattat tccttgttga cgatcatatt
    23521 cttgttttgg agagttgcaa aaaactgctc gaaccccatc atgacattgt cggggaggct
    23581 catgacgcaa gcgatttggt cagccaaatc cgacgagcaa aacccgacat tctccttatg
    23641 gatatttcaa tgccggacca gaacggatac gaagccgctc gagtcataaa aatcgcctgc
    23701 ccatcagtaa aaataatttt tgtgtcgatg catttggagc ccacctttat catggaagca
    23761 tttcgtgccg gaggagaggg ctacgtgcca aagcaaacgg ccggcagcga attattatca
    23821 gccattcaac aggttcagga aaaacagaga tacctctctc ctttaattcc tgaggaggtg
    23881 caatatgcgg ttctggccca gatggatggt atgccgggta ccgaactatc cggaaaattg
    23941 actcccagac agcaggaggt gttaaaactt gtggctcaag gtttctctgc caaggacatc
    24001 gccaacgtat tgactatttc tcaaagtacg gtggcgttcc ataaaatgca aattgtgcag
    24061 gcattaggtc ttcattccaa ggccgatctc acgaaatacg cggtcaaatt aggaatttct
    24121 cctcttgatt agggattgag cggacagaag cttctgagat tagagggcgt ggatgatcgc
    24181 aggatgtatg gcagaagtga ggaggaaaag tctcggatgg gagacgggaa gaagaacttc
    24241 tagcagctat taaatggaaa ttattccggt ggcaatggca tatttcgtga gttcagatgc
    24301 ggtcttgagt cccagaatct gcatgatccg ggttttatga aattccacag tcttgacggc
    24361 cagatttaat accaccgcaa tgtctttatt ggatttcccc tcagcaacca gttggagtat
    24421 ttcaacctgt cgttgagtca atggaggagt ctccggctgg gccgtcttct ttgaattaga
    24481 tgagggattc agggcttgac tgacgatccc ctgggcaatg gatggggaaa cgtaggtacg
    24541 tccctgataa acctcattaa tggcaaatac taattctgcc gtcgcagatt gttttaagat
    24601 ataccccgcc ccgccagcct taaaggcttc ctggacaaaa tggtcttccg aatgcatggt
    24661 gagaaaaatt attttggtct ggggttcgca tttttttatt tgacgggccg cttccaaccc
    24721 attcagcagg ggcaatgaaa tatcaacaat cgcaatgtcc ggtgtatgtt tcttgacggc
    24781 ttgaactaaa gcatgaccgt ctacaacggt ccccaccaat gtaaaatccg tttccagcaa
    24841 tttggtcagt ccctcactta ccaggacgtg atcctcagct aaaagtaccc gagttttgct
    24901 catgtcagta gaagattgaa gaatgaattt ttaatataat ataaaagctt gagataggta
    24961 ttatgaaaga atcactcata taagtccaca atgagcatca agattggttg tggcaaacct
    25021 ttagtatggg gggatcacaa aacctggcct tcggcctgtg aactggtgga ccgatcaatc
    25081 cgacccacct gacaacctat cttcgaagcc gaaagagaaa ttgctgaatt ttgggtatgg
    25141 ggggacgtgt tggtggagga ggaggaatgg gcggttctgg aggaggagtg ggaagcggtc
    25201 caggaggagg aaaagggttg ggattggggt tagggatagg attcggattg gcatagccaa
    25261 agttaaaact tgataggcac cagccattga atttaaagct attcatgatt gaccctccaa
    25321 acgcttggca aacacatccc aattaacgtg ggcaaggaaa cgttcaatat attcgcctcg
    25381 ttccgatggt ttaaaatcca tcaaataggc atgttcccac acatccataa cgagaagagg
    25441 tcgaaaccct gctacattgc cgaattcatg aagactgacc cagtggttgg aaaccctccc
    25501 atttgcagga ttaatattgc acacagccca accgactccc cgcattttcc cgacgcttga
    25561 aaagtctgcc ctccaaatgt caaaactccc aaaactatct tcaatggctt tttgaatcga
    25621 tatctgtccc tgttgtgggc cttttgactc ctgggatgac aaattttcaa aatataattc
    25681 atgtaggacc attccattat attcaaatcc caatctacgg gtcagttcag aatatgcagg
    25741 catttcctcc tgatccacct gtccatcaat caaaaattcc ccgatttgcc tggtgagaga
    25801 attggtagcc ttcacgtatc cttcatagag cttaaaatgc atctctaagg ttttatctga
    25861 aataccggtt aatcctttga gggggaattc cttgaccgga tagtttttat cgttctttgt
    25921 cttcataggt gagatttctc cttccccaat cacaatttat tgctcattcc aagagctttt
    25981 agcgaaatac agccccgtca ctttcgttga aattacatga aattatgctg ttgtataact
    26041 ggaaaaaacc cttgttccgg gaatctccgg tcggggaaaa aacatttcga ccagggtttt
    26101 gcctagtcgc cttcctctcc gctatggagt aagttttcaa aaagttaaaa agagtgtcag
    26161 aggatttatg aatatccaat tcaccatatc cgatgaaatg ggcaaggaat taactgccct
    26221 tcaatccgcc ctcgggatga agggggttga agatctcaca attcatgcgt ggaatgtttt
    26281 gggctttctt attgaaacca ttcgcaacga gaaggggcca ctttcccctg aggcgatcca
    26341 ctggatttcc caaccgacga tggtctcgtc tcccaccgaa actcttcagg aacatgcagc
    26401 cttatcgcat gcggaaattg acacacgaat actggaggaa attcatcggt tgcttgatga
    26461 tgaccagctt gatcaggcat taaaagccac acatattctc aaaatcctca aatcccacgc
    26521 cgtagggaga tcgacgaatc accaggtgaa atccgagatg cttcttgcag ccgcggaaac
    26581 atttgcagcc gaatgtgatc ctgaaaaagc cgcccagctt attatcgatg ccacccatga
    26641 acaccacctg aacaccactg atggctccac cgaccgaggc taactcatat tttcttcatg
    26701 acccctcttg ccaagacatt cccacaggct ctgatattgg tttgtgattt gtccttcaaa
    26761 actggtgaga cgccctcctc ccaccccctg cacaattcct gggtaaagaa acctgggcgg
    26821 ttctgatttg gggcacactt tccatcgggg gatatctttt ttgttgaata atttcaaagg
    26881 gatcaataca ctacgtttcc gaatactatt tcttgcagga gtccgcccta taattaaaat
    26941 ttcttgagat gcattaatgg cttttggagg taacccgggt gctttccaac gaaccaaaat
    27001 ttctcattac ggctaagatt aaagaatttt ccatccctcc tatcaccgac ggactcatca
    27061 ttggtaagga tgcggctatt ggtgtgagcg ccttgcagaa ggcgctgcgg ttgctcatcc
    27121 cggaagaatt tgaccatatc gaaattcagg atgaagttat ccatagcatg ctgatacgat
    27181 cttccatcac cagacgatta acacaaaaac gcgtggtgac tttccttctt gatcaagtaa
    27241 agccattgat ggtggcggat gatatcctcc acattcagtt ggacacagaa atcaccatta
    27301 cccaagaatt gtagaatggt ctcgtggttc atttaactgc acaagatgcc gtcagtccct
    27361 ccgttttgat ggtatccgca gagatgggcc tgcgtgacct caatctgaat cataaactac
    27421 agagttatga agcctatgcc gtattttctg aagtcgtgaa ccagaagcct tccgagcaat
    27481 ttctgggcat cgtccccaaa catatgatag ggcgatatcc ttataggatt tttggggatc
    27541 ttctctcatg ttccacgtcc acccaggtcg aaccctccac tcctcttgat gtgctgtacg
    27601 cagatttcca acgacaccat attgatgcct tctctgttat agataccggg cgggggttta
    27661 ttggagccat tacgcccaca agcctgcttg aaaccctctg gcatcgagaa caatttctga
    27721 ccacacgact tcaaaaagag ataaatgaaa aaaatttcgc aaagcgagaa ctacaacaag
    27781 ccaaagatga gttggataat catgtaggat cccggaacaa tttagttttt gaccatacac
    27841 aacagctctt aacgctttcg gaaagcctca tctcaaccga aaatcgggaa cggcgagccc
    27901 tagcgggcga gcttcatgat catctggccc aaatgttggc agtcggccga ataaagctgg
    27961 ctcaaggaag ccacttgaca tcagacccgg aagttttgag tcttctcaat accgtcgatc
    28021 agttccttca ggaatcattg agctatacac gaacgttgat gacggagctg aactctttta
    28081 accttcatca atcaggccta ctgaaggttt tagaggaact gactaaaaag atgaagttgc
    28141 ttgggttggt cgtcaccctc gaagccgaac gccctttgcc cgacctatcc gaaaatcagg
    28201 attttcttct ctattggtcc cttcgtgagt tattatttaa tgtgattaaa catgctcacg
    28261 tcgaacaagc cacgatcagc attcaccagc ttcacggcac acaccttcgc tgcatggttt
    28321 ccgatcacgg atgcggcttt gatcctgcaa ccatggcggg ggagattccc ggtcaggatc
    28381 atttcggttt atccggcatc aggggacgaa tgacggcgct tcatggaaca atatcgctga
    28441 attcttatcc aggccaaggc acacgggtct ctctcaccat tcctttgtca ccaccttctc
    28501 ccccacattg attctccatc catacctcaa gggtttcgat cagtttcctt tctctttcaa
    28561 cttgatatgt tttaaagaca ctcctctgcc agacgaccgg tagcagcccg cgaccggaag
    28621 gaatcggatg agagcttttc ctgcgtaatc tctcagaatg acagaaacct cgaaacattc
    28681 tggagtcgct tatgaaatcc ccatttaaat attttgttgt attggtgacg tgtccattat
    28741 tcatcgcaac cataatggaa aatacagaag gacaggtcct ggttgtccct ctggccggtt
    28801 ttgaggaaac gatatctccg gaaacacatg ttccgcgttc cgacgaggac aataggcgtg
    28861 ggcattcaga cgaagagtta gaatatcgca ttcaaagacg cttggagatc agtccatatc
    28921 ggagtgacgg catcacggtt caagtgaaac agggcaaaac ggtactctca ggacatgtca
    28981 aagaccggga ttcgatagtg gatgtggtcg aaatcgctta tgactccgga gccacgaacg
    29041 tggacaatca aattcagctt gaccatccat ggagagagat aaacgaccag aaattgaaag
    29101 aggcggttga agccgagctt tatttgagcc catttgtgaa ttcggttccc atccgcgttg
    29161 aagctcaaag cggaaccgtc acactttcgg gaagagtcga aaatcgaggg gaaattgtgg
    29221 atgcagtgaa aaacgcctat gaagcgggag cgaagaatgt ccgcattcgt ttatggatcg
    29281 atcccacatt agattaacga aggaaggagc ctgccccaaa aacaaaacgg actagggttg
    29341 aatatcaaag cagccggcat taaagatttc ttcattagaa taattagcta aaatttttga
    29401 caaatttatt ttatttttga ccaagataaa gtttctaaaa gtccctcatt atttgggcgt
    29461 gtttttaaga tgatttggtt gccctaacgg ctcgctctca ttgattggag ccatttccga
    29521 attcaaggga tgcgtgggaa ctggggagat gtcgggaaga atttgggggt cgtctgtcgg
    29581 tgattcggtt ccaaccggag gagtggacga tgcggtgcct gaagctccga tgtcgggtgg
    29641 tgtccgtacc ccttccccaa gaatggggga atctttggtt ttgtctccta tagaaggaga
    29701 gggattttct gaacggtctt tggagtgaat ttcatcagca ggtgcttcgt tagaatcatc
    29761 cccgaagacg ggttccgcta ggggaaacat cacaacaagc atgaatccca gacagggtaa
    29821 cgacactctg aagacctggt cggcaacggc tgtttttttc atagtacatc ctccattttt
    29881 cctgcgaccc cggaagcccg ctcatcaaca cttcattgaa aagcttttct tcacaaggtt
    29941 cgtaggtatg cggaaagatc cgccttttcc tcttttgtca actgcacacc agtgacgagg
    30001 gcaaaaaact ccaccgtgtc ttcaagagtc aagagacggc cgtcatggag atagggtggg
    30061 gaatccttta ttcctcgaag agggaaggtc ttgatagatc cctcggccct accatgataa
    30121 aaccgttcca ctttcaaatc gtgcatcagg ttatcggtga aatagggagc agtatggcag
    30181 gctacacact tagcttttcc ggaaaacaag gcctcccctc gcaactcttc ttccgtggct
    30241 tttttaggat tcaatcggcc aaagaggtct aatttcggcg caggggggaa atcgacaagc
    30301 ttctggaact gtgccatttt catcacttcc gtgcgagtca aatcacgacg accctttttc
    30361 tcggcagtcg ccagatcatg atcaaaatac gctgaccggt tttcaaattc agagaaatcc
    30421 tcaacagacg tcatcgcccg cttggatccg aagacttttt ggatattgac accccgtaag
    30481 gagggcgttt caatgcgaaa cctcgaaaat tgcggccggt tatcaggatt taaatgaatg
    30541 gccccattcg tatgcccgtt tacatggcaa tcgaaacaag ccacaccacg actgggcggc
    30601 actgtttttc gatcatccgt cgcattgaat tgctgctggg ggattttttc gaccagtaat
    30661 cgaacgccgt caaattgcac tggcgagaga atgccatcga acaacgcata aaaattttcg
    30721 ttggtgattt cctgaccttg tgaaacgtct cccagatccg gcctgcttgt gaggaataga
    30781 ggcggaggaa attccggcaa aaaatgatcc ggcaaatcat gaaccacgtc gaaacgttcg
    30841 cgctcaggat gaatatcggt catcattgaa ggaaacacca ttccccctgt ggaatgaagt
    30901 ggatgcggca acggcaagta cgggaagaga tcgctggctt tgatgtcctc ctccggcatt
    30961 cgggcgagtt gggcaaaggt gagccctttt ttcaagcggg ccgtgggtcc tttaggaacg
    31021 ggtttccccc gagtcatggt cacccctcca acaaattgcg gagacaaatc ataccgttcc
    31081 tcaagcagat cttgctgctg ggacctaatc ttcgatcgct gttgggtttc cctctcaatc
    31141 accttttcca atggttcatc aggactgagg tcaaagacaa aggaactggg agttcgacca
    31201 ccaaaggtat gaatagccgg agggttggga gttcccggtt tggcatccgg cgattcttgg
    31261 gtgtccccat ttccaaccgg ttgatattgt ggatacgccc gttgccaatc cttgaccaca
    31321 gccggaggcc cgatttgagg aaccagcttt cccgaaccgg gcacagcctc ccaacttttt
    31381 ggcttgccct gatccgcagc cagtcccatt cccaaaccgc tactagcgat taagatacac
    31441 cccatgatga gactccggct catccgccat gtcacttgag aaactgttga atagatatcc
    31501 attgttctcc ttattgtatg gccttttttt ggaacacact gtccagaaat aaccgggaaa
    31561 gttaatcgga aaatggttgc ttatcctatg gcttttccca caaggctttc ccattttcat
    31621 caaacagccg gacaagggga ctcccgtcct tatttaacac gacttgaact ctaggattat
    31681 ccagataatc tctcaacaca aacgtgggaa ggtcatcgct ctgaacttcc aatgccaccc
    31741 gccgcttaca atcctcgcca tagagggtta atccagttcg ctctttgccg tacacagcaa
    31801 gagaggttcg acggtcacag tgattatccc ccataatgag tgctggatgt acaccatccc
    31861 acaggctaag cgtagcccgg acctttccct cctcgttcac gatattaagg gctgaagtgg
    31921 agatcggctt tgttgaattt tcagaagtca atactgactc ggtcgggttg ggagaagcca
    31981 ataacattcc cccaatcgac cccaaaaata atgatccaag tactacggcc aacaacgtca
    32041 attctttaaa aggcataggg cctccctttg ttttccgtgg caaacaaatg atgaacctga
    32101 cccctgctat agtcttcccg taagagcaag aatcagcacg atcaccagaa ccaatcccaa
    32161 cccaccactg gggtaagccc cccacgatga actgtacggc cagaccggca aggcccccac
    32221 taataaaaga ataagaatga ctaatacgat agtagacata aatatggttc ctcccattcc
    32281 gtcatgagat tgtatgatca gtgctcatcg tctcctcact gacatggaca gaacgtggcc
    32341 agtctttcat aggcagagcc gtcttgagta gggttcgacg cgaccagtcc ccaaaaaagc
    32401 atcgggactt tccactctgc cccgatccat tgatgcctgg catgtcctct tcaagaattt
    32461 tgttcatggt ttcgttctcc ttattgctaa tctaatttga tatctgtcag gccccggttg
    32521 ttcatttcca aaattacatt tgtctcgttt tcttgattgt cttattacta caaatttttt
    32581 ggatcggaag tcctgataaa aaccctggaa tgagtgaaca atgcccctgg attctcctgt
    32641 gcttgcacct tttgggtatt tcctcttaga caaggcttat tccctgacgc tatacttttc
    32701 tcgtctacgt actttcacga catgtctcga atggcgttcg gtttacaacg gcgggggaaa
    32761 agctccatca ttcgatggtg ttacctctat tccaccgtgt attcatatat ttcagagtca
    32821 gaccctttta gaaaacgagg tctcccgctg attggacgaa tggtcaaaaa cgattttaag
    32881 aatcctgatg caatgcggga tgacacagtt ttcagatcga aaactaagcc atactcagga
    32941 acggagaccc tcctcttcag gtcggtagga aatggatgaa gtcatgacat tgcctcagga
    33001 cggaactcaa acaggcactc gctccccaaa atgggatgta agcgttttga tagccatttg
    33061 cgctctcata ggtgcgatat tcatattgga cctgtacacc aaaacaggcg tggcaatcgg
    33121 catgctatat gtgagtgcca ttatgctgac cccatggcta ccacatgaaa aatccccatt
    33181 catcgtggca gggatctgca cgattgtggc catcatcggg gtggtgtatt cacccggcgt
    33241 gaatgtggtg cattccggca gtaccattgc aggcaatgcc gtaaccaacc gtctgttgtc
    33301 gctttttatg atttgggcaa ccgccctgct cacgtatcaa tatcgtcaag ggatggaggt
    33361 cagacttcgc ttagggtcta tcgtggaatc cacgcatgac gcgatcatcg gtcaaacgct
    33421 tgagggaaaa gtcaccaatt ggaacaatgg agccgaacag atgttcggat attcgccaca
    33481 ggagagtatc gggcaataca tgacatttct ttttccaccg gaccggattc ccgaagaaaa
    33541 agatatcctg gaaaaacttc acggtggcaa gcagataaag aattttgaaa caattcgacg
    33601 ccggaaggac ggtcaggaaa tacctgtatc ggtaaccatt tctccgatta tcgaccgatg
    33661 gggaaaggtt attggcgcct ctaaaattgc acgggatatt tcccagcaaa aggggttgga
    33721 aaatctcctg gccatacaaa acctgacatt ggccagccat gcggcctcac tgaagcaatc
    33781 aaatgaagac ctcgaacaat ttgcctatat tgcctctcat gatctccagg aacccctccg
    33841 caccattcat ggctttacgc agttgttggc ggaacgctat aaagaccaat tagacgacca
    33901 gggtagggaa tttattggat tcgtgaccga tggagcgaat cggatgcaaa ccctcattca
    33961 ggacctgcta aaatattcga gaatccaagc ccaggagctg aacacagtcc aggtcaatgg
    34021 cgaggaagtg cttcaggaaa tcctcgaaca tctcctcatg gtcattgaag acaagcaggc
    34081 ttcgattact catgaccctc tgcccatcat tcagacggat cggtcacatt ttcaacatct
    34141 gctccagaat ctgattacca atgctattaa atttcatggc ccgaaaccac cacgcattca
    34201 tctttctgcc cagaaaaaaa ccaacgaatg gctcttttca gtccaggaca atgggattgg
    34261 ggtggaaccg gaatatttcg agcgaatttt tcttcccttc aaacgattac atacccggga
    34321 agaataccag ggcaccggaa tcggtctggc catctgtaag aaaatcgtag agcgccgagg
    34381 gggccgcatc tgggtcgaat cccaaccggg aaacggaacc aagttttcct ttaccatacc
    34441 aaaataaacc ggagattttt catgcaaaat cctattgaaa ttttgttaat agaagataat
    34501 ccggctgata tccgcctgac ccaggaagcc tttcgggcag cgcgcctcca taataccatt
    34561 catgtcgctc aagacggggt cagtgccatg tccttcattc gacagacagc cccctttcag
    34621 gatgctcccc gaccggacct tattctgtta gacttaaacc ttccgaagaa ggacgggaga
    34681 gagattctga aggaaatcaa atctgatccc cacacgcgca caattcccgt agtcgtgctg
    34741 accacctccg aggacgaagg agacgtgttg cgtagttacg atcttcatgc taatgcctat
    34801 ctggtcaaac ccatcgatgt tctgcgcttc atcaaaatga ttcaatccct cgagagtttc
    34861 tggctctccg tagtgaaact tcctccaaaa gtaccagagt tatgagcgat cttcattctc
    34921 caacccattc tatcctgctc gtggaagaca atcctgctga cgcggaatta gtgcgggaac
    34981 ggctgaagga tgcctccggc atgtctcctt tttatatgac gcgcgcatcg acactcaacg
    35041 aagctctgac aacccttcaa aacacgtcgt ttgacgtcat ccttcttgat ttaaacctgc
    35101 ccgaaaccaa cggccttgaa acattgaaac gaatccggtc ggcaaactcg gtgattccac
    35161 tcgtcgtctt aaccgtgaat gaggatgaac ccttagccct tgaagcgatc aaattagggg
    35221 cacaggacta tgttccgaag tcttccatga atgccgtgct actttcccga attctgcact
    35281 atgccataga acgggaaaag aacgaccgga ttcgccgtga tagcgaaaga aaataccggt
    35341 tatttgtgga tggagccacc ggcttggctt ttgtcatgct tgacttgcac gggaacatta
    35401 ctcattggaa ttcaggagcg gagcggctct tcggttatcc ggaagacgcc gccttgcata
    35461 aacatttttc tatgctattt accgatgagg accaacgcaa cggtcgtccg gaaaacgaac
    35521 tccggagggc cgaggcagta gaaaaggggg acgatgataa ttggctggtt cgagctgatg
    35581 ggtcgcgatt ttgggccagc ggcgcagtga cagccatacg cgactcaaca gaccacctca
    35641 ttggtttcgc caaagtggtt cgggacaaaa gcgcccaaaa agatgccagg gataaactag
    35701 ccgaactcaa tcgcaccctg gaagaacgcg taatccaacg aacccaggag ctcactcgca
    35761 accaggagcg cttacgagcc atggcttcag atctcacagt caccgaacaa cgcgaacgac
    35821 gtcggttggc ttcggatctt catgattatc tggcacaact ccttgttgtg tgccgactga
    35881 aattaggtca gggtcaatcg ctcaccgata cggaaagcct cgtaaaggtg atccaggagg
    35941 cggatgctct cttggaccaa tccttgacct atacgcggac actcgtcagc caactcagtc
    36001 cgacttgtct ctatgaattt ggactctatg cagctctcgt ctggttggga tctgaaatga
    36061 ggaagcaggg gttgaccgtc aacgtcaacg gcgcaaactt gtcgttgaat cttccggaag
    36121 atcaggctgt ccttatcttc caatcggttc gtgaattatt atataacgcc ctcaagcatt
    36181 ccggagtcaa tgaagtgacg gtgaacctcg ccatgcaagg aaacaaccat ttgattatcg
    36241 aggtggaaga ttttggctgc ggttttatag acaatgccga agacagaaat gaacaatcac
    36301 caaccaattt tggactcttt agcatccggg aacgattgga agccctcggc ggagaaatgg
    36361 tgctcaattc gaccctgcag aaagggactc atgtcctcct gcgtattccg gtagaaaagg
    36421 gcatcgagga gttccgacca cataccgctc gtccaacatc agtgcgagcg gtcgagcaga
    36481 ctccttccct ccgttctcat tcagagaaaa ttcgaattct tattgtggac gatcacaaga
    36541 tggttcggga ggggttttgt catattctaa actctcagtt ggacttcgaa gtcgtgggag
    36601 aggcggaaga cgggatacaa gccatgaccc tctgtgaatc gcttcgccct gacgtcatcg
    36661 tgatggatct tcatatgccc aatatggacg gtctcgaagc gaccagaagg attaaagaaa
    36721 acattcctga aaccgtgatt atcggattat ctgtttacga cactccggac gtggcccgat
    36781 ggttccaaga ggcaggagcc gaaacttttg tcacgaaagg aggaccggcc gaaagcttag
    36841 tcaccatggt cagaaaatcc tggcatcaga aaaagcccgc ctagcctttt ctctcctact
    36901 cgttttcatt cttcgccatt cgtccgcctg ctttttcaac gttgggtagg ttcgcccccg
    36961 tcagaatctg ataggcttga aataccgtat taacaggaga aatctgcatc agaaaagggg
    37021 tctgccaatc attgtcctca ggtaaacgct cttcgggaat aatcactctt cggatatgtt
    37081 ccgcataggc agcctggatt ttcaatggta tccctccgac cgccccaata tggccatcac
    37141 gggtcacttt cccggtcatc actcggctca ggaggatagt attatcgttg gccatggcta
    37201 gaactacgag actcaccatt gctgtcagac tatcgccgta tatcgtcaca ccaggattgg
    37261 gaaattttaa aaatatatcc caggtctttg gatccaggcc cgcaacacga gccgcagcca
    37321 cgatgccgga ttgaatagcc acctgagtaa aaggcgaaaa ttttcccggt ccgctggaaa
    37381 aggacacatt cagcacttct tgaccttcct gtttgcggaa cgctatttca agttttccca
    37441 caattccttt aggctgatga tgctggttca gtgtggttcc cagaaatgtc acggtttgct
    37501 gctgccaaaa cgacggtgcg gcctcggcga gacgagggaa agagcagaac cagacaaccg
    37561 ggatcgccca ggccaaaact ctgaagatga gacctatccg tataaactgt ttcattggct
    37621 aattccccga aaccggcaaa tacgggataa tatattctcg tttcccttcc actctctgat
    37681 cgacaaagtg atcgaaaacg ccatctcgat tctcgtctat ccaataaaat aatggccatt
    37741 caacagtttc aattaccgta ttccaatatt tgttattttc aaaaaaaata atttggcggg
    37801 ccgttttata atccacgatc ccatctccct tcagggagta ttcccggata tacaatcccg
    37861 taatgatatt cacgtcttca tgaataagat tggcttcccc aggttcattc ggcatatctg
    37921 cagccattcc gtttcctcca aagaggagac agaatacgac tccgaaaatc cagtgaaaac
    37981 agggtttgtt gtttctcatg atatgaatcc ctccatcgga cccttcgttg ccctgggatc
    38041 ctgaacgtat ctcccgataa aacagtcatt ccttaccaac tccacggcct tatcgagagt
    38101 attcatcttt ttacgcggtc atgaccaacc catttacacg aggacgcgcc tgaggaaatg
    38161 agttcaatgc aggagtttcg gtctgatttt tcaacaaccg gttcaggagg atgaaaggat
    38221 cgatcacatc accacattga atacagtgct tggcctgaaa ctggaaatcc tcataatcgg
    38281 agaaaatatc catacacgtc tcttcaatca tcgttcctcc gcaccgcgaa caatgtgatt
    38341 gttgcatttg gttcatcaat tgagggaata catcggggtg atcagttcgc atggcttcta
    38401 aagtcaacgt ttgcataacg cctccttcat aaattcataa aaggttgaga attgatgagc
    38461 atgatctgac tctaaattct caccctgcaa gccactacta ctgaaaaccc ttggtctagt
    38521 ggggaagacc ccttgaatat tttttgaaag gaaggtgaga gattaaaccc aaccactggg
    38581 attccatcgc gccctttaag gtctcttgaa aaaggccgcc agcggcgttc cctgccttcc
    38641 tccttcgccg aagtagccac aaaagctgaa atcggcttcg cccttacctc gaaatgaatc
    38701 agggcagaac tggacgaaat tttttgaacc gccccgagac ctctgatagg caacgagcct
    38761 gtgggtctat atgaccttaa caactagtac tattcaacat tcccatgaca aggaagtacg
    38821 gctcaggcat cacgaagcag ttgaaggcat cgacgaaacg aacatccaca aggggaatga
    38881 aaacgattta aaaaattcgg ggaaagaaga aaagaaggat agaactcgcg tcttaccggg
    38941 agacaacttg cgggctgctg attgcctcga ccggagaggc atgggagagg attgcaggcc
    39001 cactcacagc ccgctcgcgc atgagattaa acaactcttt ctcctgggaa ttctgaatga
    39061 caacccgctc caatgattcc ttccaggcag catgagcttc ctccaccaag ccggaacggg
    39121 tcatcgattc accgagataa aaccaggcat acgccgatga aggctcgcac ttcacggcct
    39181 gtcgaaatgt gggaactgcc ttttcatatt cctgatgact gaaatacaga ctgccaagcc
    39241 tattgagggc atcaagatga cacgactcat gatccaggac agttcgatac atcatcatgg
    39301 ctcggtcgtc atagtgaagt aatttcaaag ttttggccgt ttcggtcaaa ataaaaggat
    39361 cggccggcaa ttgttgaaga aggccttcat agatatgcaa ggcatcctta taccggtgca
    39421 atgcgccata gcccagagcc atgtttttga ggatagccat attcccagga ataatttcga
    39481 gcgctttttc gtaatggttg acggcaagtt ccataaactc gggttcatcg ttcaaggtcc
    39541 ccgcaatcgt ctgcatcaga tgacccgtga gaaagaaccc catccctaat atttgaggat
    39601 caaccggtaa gcggccaggc gacccggaag aagaggaact ggcgacctga gacggggaca
    39661 tttcataagg aatatgctga tccgccaacc attgccgaat gaacgtagaa ggaatggcat
    39721 agctcatatc ggccctgagc tgaacactcc cgatcaatga gcccttcctg tccgtagcct
    39781 gaccaatata cggaaccgaa tgaacgacga tccccaagac ctggccactc actgcatcaa
    39841 caatcggacc accgctggtt cctgcgtgaa ggacgccaga gacttgaaga aaaggcatgg
    39901 ttcggccctg tgtccttgaa cggcccatgg tcaacccact gacattcacc ggtttcactg
    39961 tgccatggag catcaacccc atttgcaacg tgtctatgac ccgaggaaat cccacagcga
    40021 gaccttctgc gccatcttcg gccaaggttc cgaaaagaga taaggcccgg tcatgaccct
    40081 ggggaagatg aagaacggcc aggtcggcat cctggtcgat ggtgacgatt ttcgcatcag
    40141 taaacacccc ctgatcaggg acatagacaa gcaatttggc ggatgagatc aggacatggg
    40201 cgttggtcag aatatgtcca tcctgactca taaaaaaacc cgtcccaaaa gagagtgatt
    40261 tggtctcccg gtctacagaa accagaagaa ccgcactcga gcgggactga aggtaaatgt
    40321 cactgcgttc tgatgcccag atggtttcgg agcccatcca ggcccacaag aagatcacca
    40381 agaatagccc agcatttctg aattgaacgg ataaggacat attgtcggga gggtgttgat
    40441 tcatgggtcc cgggaatctg tgagttatga tgagaggaaa ttgagagctc tcagaacttc
    40501 atatattttt gagtgagatt accggatttt gtccatcgcc ttttctccca tccagctggt
    40561 acttctaaat attatgaccc aggtgtccct gtatgtctaa tgcacaggtc ggcgaaatct
    40621 ctataagagg taaagaagaa ggacagccgg tcattttaat gaattcccgt ttattcacaa
    40681 caaggaggtg cttcaatgac aataatgcga aattcaattt taacccttgc ctgcttcggt
    40741 cttttatcta cggccgccat gggaatgcct ccggagagca tgaacgaacc agaattacac
    40801 catggcacaa acacggatcc cacgaaccag cccggacctg agcattccga aagctccatg
    40861 ggatccgccc aagggaacat tatggggtcc gagattacag gtgaaattct tactgtcgaa
    40921 ggggatctgt ataccgttaa agattcaaca ggcaaagaag tgcaattgca tgtggacgaa
    40981 tccacggaaa agtccggaaa attaatagag ggagacaaga ttaaagccga ggtcaccccg
    41041 caaggtcacg cagtcatgct gaaaaaagca aatgacatga actaagcttt agagtcaggg
    41101 aaaaaagccg gcggattccg ccggcttttt tttgccattt tcattctacc cactggggat
    41161 ggatgcccaa acgagagaga gtcaacaaat tattcggcag atccggaaac agggcctcgg
    41221 agggatgttg caccataatc tgccacgagg gaggtcactc tctgtcaatc ggacgctcag
    41281 gataaattcc acaatgtatc tcgctgagcg ttggagcctt tcgagctgga cattaattct
    41341 tcgctatcgt tcagaatgac aatctggtgg gttttactgg actagtgata tacgcacccc
    41401 atgcctgaaa gaggagaacc aaccaataaa acactcaccc tctgtgactc tgtcatactg
    41461 gattgagagg tgaaaccgaa ggcggaagct cttttactga ctggttattt tttattcctg
    41521 atttcccaga ccactgcgtc cccagctcct ctcattgtca ttctgaaaga agcgaaggat
    41581 ctgtgcccgg ttcgacggga acatggcttt gtctgatgct tcgtcttcat ctcagcatgg
    41641 ctgtggaacg ccgaagggga aatgaggaag tcaggtgtga ggctttcagg aagagctata
    41701 gttcttttga ctggatcgaa tgacttcggc aaaggaatta aaggaatccc ctttggttac
    41761 atattcgtcc cctccagcct cagaaaagtt cttcatcact atttgatctc catgcatgga
    41821 caacccgatg atatagattg acgggtggct ggctttgatg cgacgggtgg cttcgatgcc
    41881 gttcatcacc ggcatattga tatccatcac aaccacatca ggatggagca tctgaacaag
    41941 ctcgatgacc ttttgaccat tctcggcttc tccgaccacc tgtatatcgg gacaggcatt
    42001 caacagggtg gccaaccctt cccgcaatat tggatgatcg tcagcaagaa tgacacgaat
    42061 gaggtcgccg gatttctcaa cgtcagttgg agaaaattcg gaatggtggt gggtctcggg
    42121 gtctgcctcg gggtctgcct cggggtctgc ctcggggtct gcctcggggt ctgccataaa
    42181 acgggtgtaa aggggaatgg tgatcctgac acgggtgccc tcctcttttt tcgaataaat
    42241 ctcacattgt ccccccaaac cctccaggcg ctcctgcata ttcagaagac cgaatttcgg
    42301 agatttgcca attttttcaa aggtagagtc aagatcgaat ccacatccac ggtccaccac
    42361 ctcaatacta atgatatcag gcgaggcacg agtcatctca accctggctt ctccggtttt
    42421 ggcatgtttg actacattaa agaggagttc tctcacaacc tgatacagaa taacccccgc
    42481 tgattcgggc aggtgaatag actcgtcatc aggatgagcg gtgaccgtga catgcagctc
    42541 atatcggccc attttatccg ctaaccaacg aagggccgcc agaagtccgt attcatacaa
    42601 aacgggtggg ctgatctgtg ccatgagatc tcgcgtatag gttaacgcct catccagtga
    42661 ttcctccatc tctttgatca agagagactg ctgttccgaa acaccgcttt gctgcaactg
    42721 actgactttt atttttccga caaccaataa ctgggctaaa taatcgtgca attcgttcga
    42781 aatccggcga cgttcccgtt gttcggtcag gagtaactct gaagcaaggt tccgaatacg
    42841 gtgttggtgc cgtaggagcg catccgtccg ttccgccacg cggccttcca gtgaatcttt
    42901 tatggactgc aattgtgctt cgacacccgt gcggtagacg gcgtaggcca gaatattcgc
    42961 catggcttga agaaatatga catcctcctc ggtaaacgac cttttcgacg tggaatgcac
    43021 accgaggacg ccgtgaggaa cgccctcatg atcgcggatg aggcaactca taccgctgac
    43081 cacatgatga ttggacaata atgcggggtc cgaaaaccgg gtttcctgag agagatccgc
    43141 cacgaccacc gggtcaccgg acaacagcgc atagccggct tgcgattctc gaccggcgcc
    43201 tacgacggcg tgtccgacaa tcccgtcgtt ccagccgact cctgcccgga gcaggaggga
    43261 ctgtttgcca ggtaataatt ccaggacctt gaccatttcg accgaaagcg tgcggctcag
    43321 aagcctgaca cattcatcca tgaccttggc caaatcccgt tcctgaagcg ccagtcgtcc
    43381 gaaagctgcg atcacctcaa cgttattttg ccggcgcgag aaatccgcca cttggatttc
    43441 cgccttccgg atccgggtga tatccacaaa ggtcaagacc actccctcta gcagattgtc
    43501 ctgggtccga aagggaatca ttcgggatag ataaatcctg gaatccttgc ccaacggtcg
    43561 ttccatttcc ttgaccattg aggttcgaat cacttcctga atatccggca ctaaggtctc
    43621 atcctccaag aatgccgcca attcggaaac cggtcggccc aggtcgatat cccgaaaccc
    43681 aaaaattttt tgaaccgcag gggtgaagtg tttgatcgcg agtttggaat caagaaacag
    43741 cgtggcaatg tccgatgtca gaaaaagatt ttccacgtct gcatgggact ggtccaattc
    43801 ctccacctta ctcaccaatt gggcattgac cgttcccaac tcttcattga tggattgcat
    43861 ttcctctttg gaggtttgca gttcttcgtt ggcggattgc aattcctcat tcacg
//
LOCUS       DAORAQ010000005        43897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_194_length_43897_cov_62.411934, whole genome shotgun sequence.
ACCESSION   DAORAQ010000005 DAORAQ010000000
VERSION     DAORAQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 43897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43897
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194_length_43897_cov_62.411934"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..928)
                     /locus_tag="PKM72_01010"
     CDS             complement(101..928)
                     /locus_tag="PKM72_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNP59382.1"
                     /translation="MALVVNNNIASLNAQRNLGVSSAQLEGSVARLSSGLRITRAADD
                     AAGLGISETLRAQIRSINQAVRNSNDGISLLQIADGGAEGIGNLLGRLRELAEQSASG
                     ILGSNERSFLDQEFVALRSEIDRISSVTEFNGVKLLSGTANDSLSIQIGFRSSANDTL
                     SLSLKDLNTTTLSLGSVNVSTSGNALSALSNIDSAISAVASARANIGSLQNRIDAAVG
                     NLQVAGENITAAESRIRDADIAFESAKFVRNQILVQAGTSILAQANTLPQQALALLQ"
     gene            complement(1263..1811)
                     /locus_tag="PKM72_01015"
     CDS             complement(1263..1811)
                     /locus_tag="PKM72_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59383.1"
                     /translation="MHVQLDEEIWEVSDRVQLGEVLANLSDRAQAKGCLVTELRVGDR
                     KMTDRELVPPTLAQVASSFGSIAAVSKHVETIVRYSEETGTQFGAQLCDEGQKFVDAF
                     RQGDGKFHHLDKWFGQIADYLEWLKIHEIVGLKNPEATRDLSFWVNELLNAREVEDEV
                     RIADMLEYEVIPRLVQKNPSPM"
     gene            complement(1792..3447)
                     /locus_tag="PKM72_01020"
     CDS             complement(1792..3447)
                     /locus_tag="PKM72_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNP59384.1"
                     /translation="MNKQILIINITRMGDLIQMVPLLSRLEEEWPGVGIDLIVDKEFA
                     HVASLIPGIRQVLMFDFQLLMDESRVGIRDVVTLYQDIAGWVRPLLQVGYDRVVNLTF
                     NRRSAFLVKYFGCADERGMTTTQDGSFVVKNPWMKYFVDFQVYRQLNRFNIVDLFSLG
                     GSGTGSFHPLRLHVPQDMKEWARTYLRQAGTADKWIAIQVGASDPMKAWRPEYYGQMM
                     ASLSQKQPVGFLLIGTKKEETDVQEAIRCYRQAGGLGTLCEAVGQTTVPQLVALLEHC
                     QLMVTNDTGPMHMAVGVNTPVLNVSVGHVNFWETGPFGPGHWVVQPEILCGPCGFDKV
                     CPHHACKDQIIPQEMADLCLHLMGTRPFPSFSSKIRVYEGGVNEDQLGTFTIRAGTEE
                     QAASWYAEYWRQHWYEFNTGTSSLVHFPPTSPPDFQECEQVWRRLAPQLDQLCIQAET
                     VLDMCRQRPIPVNRLKAQQHQLSVDTLTMQLVARPSLAFGPLAMAFYRDTLSLESATL
                     SGMAEEHVRAYHVFRTRGEENYRRLKEMYSHDPRRALYACAIG"
     gene            complement(3487..5304)
                     /locus_tag="PKM72_01025"
     CDS             complement(3487..5304)
                     /locus_tag="PKM72_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3880 domain-containing protein"
                     /protein_id="HNP59385.1"
                     /translation="MDVLKKNIGILRSQDPILAAQILQVPGGTLSIHPAKSGMPTALV
                     NSRYLHSAYDPVREAGRWAEERVKDCQAGETIVLLGVGLLYHVEALRQMLPHDQNMMV
                     VVPDLSEFADCVSVRSLEGWGERLMWMTGSITDMAVQVTQKAKRVRILSYEPAATVHH
                     DLYEHFRLQLRDHLAQGLSGTLHIMVIGPIYGGSLPIARYVVNALEGLGHRVSWVDHS
                     PHYSGYQNLDTIRDHRLRLTVQQRMSETLAVISLAHVAEDPPDLVLALSQAPLTMAVL
                     EQMRRKKVLTAMWFVENFRHLTYWQQMVSGYDFWFVMQQAACLDAFRKAGAKQVSYLP
                     LAADPAIHQPMILTQGAQYEFGADVSFLGAGYRNRRQILPSLVGQGWTFKLWGNEWDN
                     PGPLAQVLQRGGARIDTPTSVKIFNATSVNINLHSYTGDGFDPEGDGVNPRTFELASC
                     GAFQVVDARTLLPALFDESMMAVINSPDQLLPTVQTYLHEPARRVAMAELSRKRVLEA
                     HTYGHRMRTFLGAVGMASPDRLGAILQGDRTAESLVARSGQSPELIPMLQKFLPTDRV
                     ELVDVAKDIRKKGPEARLNREELLILMMDEYRQEKRDFL"
     gene            complement(5373..6608)
                     /locus_tag="PKM72_01030"
     CDS             complement(5373..6608)
                     /locus_tag="PKM72_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNP59386.1"
                     /translation="MSRALLIQLARLGDLVQSLPVLTALQSAKPERPLDLLCPSPLVA
                     LGELFPCVDRVYPWYGEQWHELARTKMTDCDQQLAQATRYLAEHAFPTYSLAYNLNNH
                     PRGILAAHVLSSLVVGPGENGPLNQRLPAWAGYLRQIAQDRGSNRVHLADAFCGLCQV
                     FPPSDIPYLKAPLVKLPLELEWMVNNRSSTLIGIVLGAGDTERRVPLSVWQALISACA
                     EYMPQSYLLLIGGEGEREAALALEHRLPAKYLNRVVNGCGRTSLPQLVALLNRCHWVV
                     GSDTGPLHLGAMCGTRAIGWYFSQARVHETGPYGVGHYVWQHDQGRPGDVLDARELRT
                     EESSFAHWPVMETVNLIRDERVNSRIDEWDLWISNKDEWGAFYTTDGLPDEAVLQRKD
                     TWEALSQLANHNIRMEVAK"
     gene            complement(6642..11288)
                     /locus_tag="PKM72_01035"
     CDS             complement(6642..11288)
                     /locus_tag="PKM72_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP59387.1"
                     /translation="MKSYFTSAGYLFNKDEGVWHRPGYKGINYSDGEQIENRLKTIIS
                     GASDVSVMSGELAKSCTDWHSLYHLSRKRVNLLRPFEEQLQGKSVLEIGAGCGALTRY
                     LGEIGAEVVALEGSLRRASIASLRCRGLSNVTVMGEVVHHFNPGPQFDVVTVIGVLEY
                     ARKFFPGDGADPVDAMLVFMKGLLKPGGTLIIAIENQLGLKYFAGFPEDHMGKPMFGI
                     EEHYTKGSIVTFGRRELSRRVGQAGLPEQEWWYPFPDYKLPSLMVSERGAMPQDGIDL
                     TPVLRSACTDDPQFPARIHFNQERALRPIVRNGLLPDLANSFVLLATDLACKEKSIVP
                     LAVHYATGRRPEFAKKVVFTQESQRAGYTSQVRLYPTDTPNKNSLVTQRLVDQTFVKG
                     ELWQDRLVQIMTSPGWRVTQIEEWLKVWLKGFFDVVGIQKSNDMTKKKVSGQFLDLIP
                     RNMIMEGNGEVTFFDQEWVLGEDLEVGYVVFRALFSSFYALGMIAPTGEKTLPSILSL
                     IKDIGQGAGFSFTDHGIKHYHDLECEFQRLVVGGCNLSYGMMSAMQFRVSHTQSNQDG
                     YGAEVHGQNIILQQALLEKTRQIADLTQTVKDLDKQFIRINRVLSEKELNRSLSEPKM
                     CLDDRISTSGQNLQVAPRVVAIINDPICSQIRILQALTASVMKSWFGGMVKIEGAEGS
                     HPHQLPSGPGTVWIVQRTCRIEIADMVIARSNGTRLVHDIDDLLWKIPEDNQNHQVMK
                     KWLIDHLMKLLELSDCVTTSTIPLQTALESVGIKAALLPNCLVSEEWRDLAPLRGGGS
                     RPRIGWAGQVGVHRSDLRFLGSVFEELGDEVEWVFLGEVPEDLRRTGVRSEVHPMVPL
                     GQFPSKLASLNLDLALAPLAINEFNEAKSDLRLLQYGVLGFPVIATDIYPHRTAPVTR
                     VPNDPQAWVRAIRDHLYNSDHSEKEGKVLREWVLRHRMLESWLPQYRAVWLGESANRR
                     DHSNFSVLGNNEARSTPTSYMPIVDTQYECSIIIPVCNRDDLTKQCLLHLAEVTQGCH
                     YEVLVVDNASTDETQNFLASFGGDIQIIRNTENLGFAKACNQGARAARGKYLIFLNND
                     TIPQSGWLTALLEEVETHDDVSIVGSKLLYPNDTIQHAGVVFSKNCLTPYHIFSGAPA
                     GLHAANVRREFQAVTAACFLIRREDFESIGGFDEEFRNGFEDVDLCLKIRERGKKIIY
                     QPKSVLYHLEHQTPGRKDPEAERHNGNRLMSRWGSKIVVDEDVYTVPEGYANRYYFRD
                     GWLRQSLEPFRSDGERTQWNRVQRVQELLLSRRYDPIRGIKKQDNGELHALLSDFREW
                     PDDGEVLRWAVKLCRTLQLFDGERAFLTRLLSLGEDREIREQLAKLALNVKDLSDAAQ
                     HVQALIQTDPNDGSAHWLQGILFMQSQEWGEARKSFRRALQYGSDSRKTNIGLGMACM
                     GMGDGEEAWRVFDQVVADHPDDVEAMNGLIQAGTSLQRWKDLGERLSRYVERNPANCD
                     MRFALAGVHFRASRLDLAKQQFEMLRLLMPDHEGLLDLGVLLQTAPRDLHAVAT"
     gene            complement(11833..12084)
                     /gene="csrA"
                     /locus_tag="PKM72_01040"
     CDS             complement(11833..12084)
                     /gene="csrA"
                     /locus_tag="PKM72_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNP59388.1"
                     /translation="MLILTRKIDEAIRLGDDIRIVLVQIKGGQVRLGIECPSHVRVLR
                     EELYEAVRQENLNAVSSDPKLLASLPRQKRPQAKPTEST"
     gene            complement(12195..12671)
                     /gene="fliW"
                     /locus_tag="PKM72_01045"
     CDS             complement(12195..12671)
                     /gene="fliW"
                     /locus_tag="PKM72_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNP59389.1"
                     /translation="MKVQTSRFGMLDVSDDTLLTFPSGLVGFPDFRRYLVLDPPEDAD
                     YQWFQSVDEPSLAFVIMDVDLLQPDFRTNLSGEGLAELDMTPADPISIMAVISIPSDH
                     PDKATANLRAPLVVNGRTRQGKQLILHESISLRHPLFHDVAEGQPHPEGVTEAASV"
     gene            complement(12757..15111)
                     /locus_tag="PKM72_01050"
     CDS             complement(12757..15111)
                     /locus_tag="PKM72_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59390.1"
                     /translation="MASILVINDDPVQLHLLASWLEKDYFAVNRFVCSEDAWRWLQEG
                     NVPDAIVLDLHMPGISGWRFCELLHSFFGSTQAVPPVLAVSATYTGIDAHDILKDLGA
                     SAFLALPTEPQRFRQQVCELVENPPSPQRPHVWMVSTHKSEIQRIHHIFGERGWQVVE
                     WQAGKQVQVAVNLVLPDIVLIDHPLSDMTSEEFVIWCKVQYPQAMCIVLGQESTARGY
                     PAHSIHADAYLPKACDPLHIISLCEKGRWERALSRVEHLLDIRTDDLRESEAQFKELF
                     ETLPDVLVIYDFQGKITHVNSLGAQQLGYLPSVLNGKAFSLIRPEPSSGESGPTLPSS
                     APGGARWEETVLRQKNGSNLPVEMMEREVQFLGQRQTLFIGRDLTARKRMEEENIALE
                     HQLRQVQKMEAIGRLASGVAHDMNNTLTAIMAHASLFKIQEKTDTPLWAAGDVIEKAV
                     RRGKELTSQLLGYARQGKHHDVTVDTHDVIQEVMTLLGRTIHKMITFRTDLSATKPYV
                     LGDPNQLYQILMNLGVNACDAMGERGELLVQTSNETVTQEYASRVPGLHAGDYVVIRV
                     TDTGTGMSLETQRHIFEPFFTTKGRGQGTGMGLAMVYGIVKNHRGYIGVTSSPGCGTT
                     MRVYLPDALCASLKDAPVQTTKPSHGTGHIVVIDDEKDVGEAAQAILEFLGYQVTVVL
                     NGKEALKICRDLTVPVDVVLLDMVMPEMSGATCFEELRAIRPDLKVILCTGYDRNHAV
                     QDLLNQGVVGFIQKPYDVDELAHACQIVLRDDNSVKICGTERVS"
     gene            complement(15114..16085)
                     /locus_tag="PKM72_01055"
     CDS             complement(15114..16085)
                     /locus_tag="PKM72_01055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNP59391.1"
                     /translation="MRILVVDDDPLTLHMVVYRLRQWGHDVISCTDGDSAWKVLERGS
                     VPNVAIVDWIMPGLNGPELCQKIRGRTDCPYVYIVMLTGRKNLEDLIAGLDAGADDYL
                     TKPFLLEELDARLRAGKRIVDLQNELISARETLRIQAMQDPLTQILNHGAIVDTLLRE
                     IDRAHREEQPLSLILADLDGFKNVNDSYGHVAGDHVLVEVSRRMRFCLRSYDAIGRYG
                     GEEFLMVLPNSDAAQAVRLAERIRVAVSQKPFRVHNVDLTVTVSQGVTTWTDPCPIPI
                     EQLIQSADGVLYLVKNSGRNGVEFAQFHRHADDTFSRLTISPSSLKQ"
     gene            complement(16157..17308)
                     /locus_tag="PKM72_01060"
     CDS             complement(16157..17308)
                     /locus_tag="PKM72_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNP59392.1"
                     /translation="MRVTDRQQVDALLRAIQRIQGNIGDRNEQVSSGKRVNRPSDNPA
                     ASERINQFRNVLRTTEQRLTTVNEGVGRLNLSDSVLGTAGSTVQRAIELALNMASDTN
                     TPVERRNAALEVQQIILGLAGIANTQLNGRFVFAGSQTQSEPFVPGSATGSASINNGG
                     SANIAVSVVTASALQPDAYQIQFTSSTQFDVLNLTTNQTVSSGNTYSSGAVFSFEGLD
                     ATITDGGGPPAAGDQFFVRVGYTYQGDGAGIEVEIGDGRTVETNVPGNQIFSGPTVDL
                     FQDLQDFHQALVTNDGKGIQAAIGQLNQSHSQVTDARATIGARVNRLDTVKDGLELLS
                     VNTQTLRSNFEDADIAQVASDLATLQNTLEASMSTLARQFQTSLLNFIK"
     gene            complement(17388..19034)
                     /gene="flgK"
                     /locus_tag="PKM72_01065"
     CDS             complement(17388..19034)
                     /gene="flgK"
                     /locus_tag="PKM72_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNP59393.1"
                     /translation="MAGISDIFNIARSGIRANQQGLATTSHNIANINTKGYSRQEVVL
                     ESARPAEGKIGSGVRVAAIRQTVDTFLENQLTALHEDLGSISARNNYLVQADGIFTET
                     DNSGLSLSLTEFFNAVRDVATNPESTIQRTVLLAKGQSLTDQFGTVAKGLNQIRLDAN
                     GEISRHVNTINGLATKIASLNDVIFKVESSGPEALDLRDQRRVLINDLAGLVDIEQVT
                     MKDGIGIHVGGQLLVGGNHANKLSTKSDPDNPPLHDVTFVRSDGSEYSISDKIYGGQI
                     GGLLALRDGDIVKFQDQVDRLAAVLTTEFNQQHQVGYGLDATTNNNFFSTLTPPAPLA
                     NERNTGGATGNSVTIADSTLATFQGYEVQFSGATSYSVVNTATGASVTSGSYTSGAPL
                     SFDGLDVVINGTPAAGDVFYVNAQKGAALQFGVALSDTDKIAASSTAAGVPGNNTNAL
                     DMVAVHTNRQLDLGNVTLNDYHTITIGDVGSATQKSTQALHSKQLEVDQVTALRESVS
                     GVSLDEELTNLLSFQRSFEASARMITVADELMQTMLSMGR"
     gene            complement(19042..19539)
                     /gene="flgN"
                     /locus_tag="PKM72_01070"
     CDS             complement(19042..19539)
                     /gene="flgN"
                     /locus_tag="PKM72_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNP59394.1"
                     /translation="MNHPQSAKWERLLKALAEAQAAFQQYGVLLFQEAHSLRIMDQPK
                     LVEVNSQKEQVLNSMRRLEKQVEGALHQLVGDVAQETIWTWLKANPDPQAQTVQRMLI
                     DLRRLARLIQEQGKKNESAIRPIYHRVCEAIQVMYTGLGTGPVYQGSGTLYFPSVPSS
                     VYLQG"
     gene            complement(19573..19893)
                     /locus_tag="PKM72_01075"
     CDS             complement(19573..19893)
                     /locus_tag="PKM72_01075"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNP59395.1"
                     /translation="MTIRGLDPSGELGKLFFHVQAKNLQTQEAGPSRASQHPSSADPV
                     DLSVLAQEIRDYSIRVAQFPEVREDKINRIQQALETGTQLATSNQVADALTRETILNE
                     LGTK"
     gene            complement(19955..20311)
                     /locus_tag="PKM72_01080"
     CDS             complement(19955..20311)
                     /locus_tag="PKM72_01080"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNP59396.1"
                     /translation="MIPDMFPLDMTGLSHSVSSGKIDRVAESARRGELLRASQEFESY
                     FLSYLMKVMRETVPKGELTSNPMGEMYYSFYDEEIGKRAAQSGGIGLSAYVLDTVARN
                     EDLKRSPSSGPPFLKS"
     gene            complement(20369..21583)
                     /locus_tag="PKM72_01085"
     CDS             complement(20369..21583)
                     /locus_tag="PKM72_01085"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNP59397.1"
                     /translation="MIHPSSFMKSHAKLQKFTRTWFSRWGLVLVCCSGWLIAGTGSLT
                     EPAETQAARIKDIASIEGVRENQLVGYGLIVGLDRTGDSVVGGQFTAQAIISMLNTMG
                     LKLNIDPIQLLTKNTASVMVTAKLPPFARPGMKLDVQVSSMANAKSLRGGTLLLTPLK
                     AANQQVYAVAQGPLSTSGFEAGAGGTTVKKNQQSGGIIPGGAIVERSVDLDMQAWEKF
                     SLTMHQADFVTALRVSEAINAHLGDGVASALNSGQIQVGVPERFQGKIVQMIAAVEGL
                     DVHVDVLAKVVVNERTGTIVMGEHVRLASMAISHGDLTIKVGTQFNVSQPETAFIGRG
                     AQGAGRTVVTPDVQTDVQEEEGRVIQVDSSVTLGDLVKALNSLGVTPLDLVAVLTAAK
                     AAGALQADLELI"
     gene            complement(21634..22335)
                     /locus_tag="PKM72_01090"
     CDS             complement(21634..22335)
                     /locus_tag="PKM72_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNP59398.1"
                     /translation="MSVTRLITRFGAGGFALLIIVSGWTGCAKEKTITQIEAEPNIHK
                     KDTPSSLGSLWDPENGRAFMFEDRRAGRIGDIVVVQIVEQHRGSKKANTKADRDSSLS
                     AGVSGGLFGINTLTQKFAEYFNIDAGTSHEFEGDGSTSREDTLTGTIAANVIEVFPNG
                     DLRIRGKREVTVNSETQTMTIKGIVRRIDLDTENMVLSSKVADAEISYTGLGVVDDVQ
                     RPGWATRIFDWITPF"
     gene            complement(22332..23084)
                     /gene="flgA"
                     /locus_tag="PKM72_01095"
     CDS             complement(22332..23084)
                     /gene="flgA"
                     /locus_tag="PKM72_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNP59399.1"
                     /translation="MPSILLLGLLLCGHGWWTAEGMAWAEELVSPTPVRLNTQTVGVK
                     DFERVIVSELTRRYGRPHHQVSFRVLFPKQLVEVPKGKLHLEIGAWSGGGRTGRRAFR
                     VGIFVNQQFLQTVNVVGEVKAQVEVATPIRWLKPTEVVTVEDLSSMMVDAPSLTHDFI
                     LDLDEVVGKQVLRPLPPSQPIRKTMLDDPPMIHKGDRVMIEVRGEGLLVQTVGVAKSA
                     GKRGETIPVKNQTSGREVLGTVMASGLVEVGF"
     gene            complement(23099..23890)
                     /gene="flgG"
                     /locus_tag="PKM72_01100"
     CDS             complement(23099..23890)
                     /gene="flgG"
                     /locus_tag="PKM72_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNP59400.1"
                     /translation="MIRAMHTASTGMSAQQLNIDTIANNLANVNTTGFKRSRAEFADL
                     LSQIARLPGASASNVGVFPVGIQVGLGVRPVSVSKEYVQGSLKQTTNPLDLAIEGVGF
                     FQVTRPDGTTMYTRAGSFKQDNTGNVVSGDGDQLIPSITIPSGALQVDIGQDGTVSAL
                     LPGVSQATQVGQIQLVRFDNPSGLIAQGNNLFSESSSSGPAQQGTPGFSTGFGNIQQG
                     FLEISNVNIADEMVNMIIAQRAYELNAKAIQASDEMMQVVNGLRR"
     gene            complement(23981..24700)
                     /locus_tag="PKM72_01105"
     CDS             complement(23981..24700)
                     /locus_tag="PKM72_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNP59401.1"
                     /translation="MNRGIYPPLAGAITLEHRLEVLSHNIGNVHTTGFKKDKPVFATI
                     LGQTSGPSVAGIDLFPTMDGLPPDRSQGALFQTGEPLDVGLEGNGFLVAQTPDGLRYY
                     RGGKLYRNANGNLVAHTGDPLLGKKGAITLPPGDVVIGVDGTLRVNNQVVDKLRVDTI
                     ADGQETVKVGNLFWTVPDQVVADKQTTVHQGMLEQSNVNPALDMVELIKVTREYEQMQ
                     KAIKAMDELAAQAIQAGRVQG"
     gene            complement(25031..25771)
                     /locus_tag="PKM72_01110"
     CDS             complement(25031..25771)
                     /locus_tag="PKM72_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNP59402.1"
                     /translation="MKKKHEEHENHERWLVSYADFITLLFAFFVVMYSVSSVNEGKFK
                     VLSESLVSSFNNKKTVGELSIVSLPINRNTPIQVKVKPSSGDNARAFLSVANAITAAN
                     IPQGVQITTTERGLNIRIKDDALFQSGSARLNPQIKEFIDLIAGLVKELPNSIAVEGH
                     TDNQPIRSSLYPSNWDLSTARANTLVRYLIDQHHLADYRLSSTGYAGTRPVELNDTPQ
                     GQASNRRVELIVLKNTPSDTDSSHPYLP"
     gene            complement(25811..26569)
                     /locus_tag="PKM72_01115"
     CDS             complement(25811..26569)
                     /locus_tag="PKM72_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNP59403.1"
                     /translation="MDILSVLGIIVSLGAIVGGQHLEGGHLSSILQGTAFLIVMGGTL
                     GAVMLQFPLPIFLKALSSASMVFGNVKVDMKGIIAKIVELSNISRKQGLLALEGQIKS
                     LADPLMAKGVQLVVDGTEPHKIKEILEVEVEMFEEEFGTTSKVYEAFGGYAPCVGIIG
                     AVLGLIHVMENLADPSALGGGIAVAFVATVYGVGAANLLFLPMGSKIKLKAKTLIIGK
                     IMVIEGLVAVAQGENPRMIEEKLSGFLPSSEKKK"
     gene            complement(26589..27074)
                     /locus_tag="PKM72_01120"
     CDS             complement(26589..27074)
                     /locus_tag="PKM72_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNP59404.1"
                     /translation="MQNTLTKVLPHDDFGEDCGVVLQMVSFQLGDEIFAIDILGVQEI
                     IRMLDITQVPNAPHYVDGVVNLRGKVIPIINLRARFGLSGTEPTKDTRIVVVEVAHMI
                     LGFVVDSVEEVLRLSEECIEIPPPTGRGGAEECHKGVGRVNGSLVLLLDLERLFNNQT
                     F"
     gene            27530..27820
                     /locus_tag="PKM72_01125"
     CDS             27530..27820
                     /locus_tag="PKM72_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59405.1"
                     /translation="MDRLASSQADRLLLDAKKAILQEQHQRFQDLQLEGRWDDAWKQL
                     HVTLACAADLLKDSVTMLEQVVVQKKLPPPPDQSTGFNGSINQNPLPPHKDD"
     assembly_gap    27996..28095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28096..29680)
                     /locus_tag="PKM72_01130"
     CDS             complement(<28096..29680)
                     /locus_tag="PKM72_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNP59406.1"
                     /translation="MQCLRIGHKLVLILGTLAVGMIVCGLTVIYQQELGRLQVNLEEE
                     GRIIQAQLEVTRAYIAKNYVGKIKQSSMGPHLHVSRDHEQDPDAVPFPATAIQEIGKE
                     LRLAGGDQARYVSDQPMNPANAPKDTFEQKALDLLKNGAKGLSEIETINGVPTFRRVS
                     ADVATVEACVSCHGGKKLGDVLGMLSLSIPITQAKEAMVSSVLHSGMWLIGIVGIFVS
                     AVHWLVYRLVLKPLYALMGICRDIAQGEGDLTKRVPVGNGSDEIVELSHDLNSFIEKM
                     QLALSLVSHATNRLVTSTVRLSATVDGVVSTAEGQDGRFLQSASVVKEMTRTAGEVAR
                     HSTEAARIAQETAQTARGGQNEMKQSVMDMQQVSHAVVHAANIITALGRSSDQIGEIV
                     RVIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTTRATKEIGDMIRE
                     IQKDTRSAVSSMEQGTSQVGQGVALAKKTGEALANIHSMINATAGMIQQIANATEEQS
                     TATRQIASDLESMTQATRQA"
     gene            complement(29721..31826)
                     /locus_tag="PKM72_01135"
     CDS             complement(29721..31826)
                     /locus_tag="PKM72_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNP59407.1"
                     /translation="MRKPTWNVGITTKLVWILMFFSLIPLSVQVYSLFQTGEVLKKEV
                     GVQYEDVAKVIVEKISLYLAERSADAQILSRSPILGGAELWEQAVRPSQEFVQVLNAY
                     VQATGMYSLVQVVDLDGNLIAVNDQDAEGLPLRTEKLYGKSYRLTPWFRALQQGEESI
                     RLTESSLTRNVPQEVFVENVMVDQDVQALFPQESSLTIGLSVPLYAKGRVVGYGSHRM
                     KFSKIEAFFQEAYLDLKKAGFPHAELTLQDGKGMTLMEYAPAVHGTEEMIHDLENVVF
                     KENLPQLGLEAAQLAIQGQSGNARNFHPGKNANQVIGYSPMSGAGGSLGMHWSVLVQV
                     PENEAFSHVRSLTNKTLLEMLLGLLVVVPIGIFMGRKVVSRLKPVWEVAAKASKGDFT
                     HRVPVKTQDELGQMGLALNNLLDELNRMLLQTRNVAHSLSQASVQLSSVGHQVVRASQ
                     SQVHQATQVAEAVEEMSATADDMARHTQALASTASGVNDSAVRGGDIVVSSIHGMESV
                     SNRIQESASRIQNLGQRSKDIGDIIGVIQDIAEQTNLLALNAAIEAARAGEQGRGFAV
                     VADEVRKLAERTGKATKEIATVIESVQAGTHEAVLSMEAGTEEAHSGMVLAREAGSRL
                     TEIVQGVQRVVDMIQHFAESTKQQSAVSGKISSSIQQVAQLSQDNESHIQGVATATKQ
                     FAALAEDLQTSLSRFTLKR"
     gene            complement(31910..32866)
                     /locus_tag="PKM72_01140"
     CDS             complement(31910..32866)
                     /locus_tag="PKM72_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNP59408.1"
                     /translation="MAKWMAEIDQRTGLAGANKMELLMFHFGTNETYGINVFKIREVM
                     KLPPVVKIPDSDPRILGLVNLRGENIALVDLKQAIGLGPLDPVGAKLIIAEYNDNKQG
                     FLVGGVDRIIRMSWERIQIPPPMVQSSRQGAVTAITKLEDGRMVLILDVEKVLSELHP
                     RSEEEVLVGIELNDELAGKRVLVADDSLVARKQITKTLERLGMKYEETQTGKEALTRL
                     RQYAELGDKTDRTIFDFVVGVITDIEMPEMDGFSLTKAIREDSRLQGLPILMHSSLSG
                     QCNVDKGKALGVTDYVTKFHPTDLRDKLVQHLGNSSGVVVSS"
     gene            complement(33021..34925)
                     /locus_tag="PKM72_01145"
     CDS             complement(33021..34925)
                     /locus_tag="PKM72_01145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNP59409.1"
                     /translation="MNDEMKEILNDFLAESSEMLEALDQHFVKLETEPSNSELLNEIF
                     RCMHSMKGSAGFLGFTHLVEVAHQAESLLNKLRQGEMSVSPFIIDIILEAVDAVKMLQ
                     SDIRETGEDSHVETFGIVNKLALTMDSAEDLMLSVSPPHKAEKDTVTSAPSPPTAETM
                     LSPVVVPPETSSWSSLREAPQHDVPAGIEDVAESAGASDCPVGLSDVLNKMKEATTRT
                     VQAANAPAGKASGGKEEDQTVRVETKRLDHVMNLVGELVLNRNRLIKLGNGLEEKEET
                     SPAIRELNVTLAQLNLVTSDLQLAVMKTRMVPIRKVFSRFPRLVRDLAGKLGKQVRLE
                     LLGEDTEVDKSVADELSDPLVHLVRNSMDHGLETAADRKQHGKGSEGYVRLSAHQEGN
                     SIVIRVEDNGRGLQVEKIAEKALEKGLVAQAELEVMSPREIMNLIFLPGFSTADQVSD
                     VSGRGVGMDVVRTNISRMNGSLELDSDPGQGSRVTIKLPLTVAIIQALMVEVECATFA
                     IPLASVVEAVKVTKDEIKSINGQAVLNLRERILPLLDLGETFHIPRDRTGVECYVVVV
                     KIGEQQFGVVVNRLRAQEEVVIKSLGEFLANVKCVAGATITGDGKVVLILDMADLVRE
                     VQTSTYTGMR"
     gene            complement(35014..35655)
                     /locus_tag="PKM72_01150"
     CDS             complement(35014..35655)
                     /locus_tag="PKM72_01150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNP59410.1"
                     /translation="MLVDRMIETEVPGDGEMPEEVLNEKRSINAEMEELAKYVEVITK
                     TIREMESPVTSTSDQLPQATSHLYDLAKMTEDGTHKVLSLTEELERNREGIQHHLEQL
                     RANVAGRAVKDLDAILVMVKADEGRLLNIHVALSFQDLVAQRVAKLVAILNEIQHKLL
                     KLVVIFGIQQQKGETGASGEGRGYEMLRQLESSKTTALQQDLVDNIMSEYGMS"
     gene            complement(35701..36102)
                     /locus_tag="PKM72_01155"
     CDS             complement(35701..36102)
                     /locus_tag="PKM72_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNP59411.1"
                     /translation="MIDTGMKVLVVDDMSTMRRIVKNVLRQIGFSDIMEAENGQDALT
                     KLKGGGFGLVVSDWNMPVMQGIELLRAVRSDAELKTLPFLMVTAEAQKENLIEAVQAG
                     VSNYVVKPFTAEVLQGKLEKIFANVQPAKTS"
     gene            complement(36637..36864)
                     /locus_tag="PKM72_01160"
     CDS             complement(36637..36864)
                     /locus_tag="PKM72_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNP59412.1"
                     /translation="MKDLLTLSEVSTFLKVPKSTIYKLARERRLPGHKVGKHWRFVRE
                     EIEAWVQNAGGESVMVGAGPQPAARDRFSLN"
     gene            complement(36985..40020)
                     /locus_tag="PKM72_01165"
     CDS             complement(36985..40020)
                     /locus_tag="PKM72_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59413.1"
                     /translation="MWANSLLFQTLWDGLMIGVCLVDRNGLLTHMNLAGSRLLGWGAA
                     CPTNVSCHDLLECLVPCEKNHTEICPLSGLLLEKTMLWVPRTRLRSRQGTWCWVELKG
                     LVVDDVEASGYLLMFRDLSSEMKLTEETRRLASIPKENPFPVIEVDAAGQLLYANPAM
                     VRLMEEAHIGQDGFSTALPDRFLDLARRCLSQKHLEMHYEVSVGERQYSWTFAPHADL
                     GLLRGYGMDITEPKRAAAEMSAFADTLEAKNHELDHALIKAESATRAKAAFLAVMSHE
                     IRTPLNGVIGMAEVLLASSLDKEQQECVNIIRMSGEGLLTIINDILDFSKLESGQLVL
                     ESIGFDLTSLFEEVVDLFSERAHRKGLDLAAYVDPDVPFELFGDPHRLRQILCNYLSN
                     ALKFTMEGSVLLRGSLIHPANPACNGSLHGLNDRSGESGEETQLWVRLSTQDTGLGVS
                     EEVQEKIFHVFTQADSSMSRKFGGSGLGLAICKQLAELMHGTVGVRSQPDQGSTFWCD
                     IPCRASRTIHAPRPDVRSVAGKEVWVIGPQQSSGWVMGQLLQEVRVKVVQIESLQQAN
                     ILFESVHESERHVAGVILSNRLEKAAVREWVGRMRSFSLFHDVKVLGLKPFWYGKDDE
                     EKSLGFDGMITLPLHRKQFFQCIFGEMDDPGPSDIPGPPERLSVEKVQGTLEVLEQPL
                     LQKQAGPSVLVVEDNPVNQRVAAGMLGKLGCQVTIVDTGSQALTLLQSTDVDCILMDW
                     ELPDMDGLAITQHIRELEHAGAFLHTSAYWHRHYGSASPPVHHIPIVGMTAHVLSEHG
                     QQCLENGMDDCLFKPVHLRDFERILRKWVGFVPGGVIDPSSSKTDNKTDRKGTFSVAT
                     RDLDREHHAGNPSEFPEDRTLYDVSAALHALEGDEGLLYSLFHIFKVTTPDLIKGLHQ
                     AIHMQNRSELQRLAHQMKGALGAVHAMSEWKEAEQLECRAVSATFSLLHSAVIQLEQR
                     VKQLICVFERLIFVQGEKEGRSSQTISFAEGGTRNDHVS"
     gene            complement(40024..41292)
                     /locus_tag="PKM72_01170"
     CDS             complement(40024..41292)
                     /locus_tag="PKM72_01170"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNP59414.1"
                     /translation="MPTRKRLACSQICAGMYLVRILDSWWKSPFFRHRRLLSSHDVKQ
                     LRQSGIQEVEIDTAKGLDVSTDAESFPVEDTQECHPELETSSSQADPYSQAPRSYAPE
                     GKDGREDEHQERLIRLRGDTIAALEDMFEGVKTGQIIPHIAMQEAAKALVEKALAHPT
                     VVAEILLIEHLEQFDPTLYSHVVDTALLSILVGLQLRWEVNQLEEVAVAALLHDVGYM
                     RLPLNVVQARWGSMGIDYSLLQQHVDMSVALINKKSQFSQEIVQIVQEHHAYLDGSGY
                     STVMGGNPVSDSGTLLGLTDYVDELLAVGNSSGSFPVALAIRRVYQEAKSGKFPTRFV
                     EAMIRVLGVYPVGTVVQLSTGEDAVVVKQNPEMSVRPQVKIIKTCTGEILKKPEVRNL
                     GIPSELKYEVKITKVLDSADSSINLREILS"
     gene            complement(41586..41933)
                     /locus_tag="PKM72_01175"
     CDS             complement(41586..41933)
                     /locus_tag="PKM72_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP59415.1"
                     /translation="MNVTQEKIEKAVVMHLAGRFDFGAKKTFKDSLDEAMKEGSPIVL
                     DFGQVSFVDSSALGLLVIAHQTLKSRKIPFSLVNPQTYVRQVLDLANVAKMIPIYQTI
                     DEVPQLPVVPSGV"
     gene            complement(43001..>43897)
                     /locus_tag="PKM72_01180"
     CDS             complement(43001..>43897)
                     /locus_tag="PKM72_01180"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNP59416.1"
                     /translation="DVEMPKMDGLTFLQKIMAARPMPVVMVSSLTEQGAATTLQALEA
                     GAVDFVTKPTVDIQHGLSDLAHQIISKVKIAAQASVKSRTPPADCTERIKLLAAQSAM
                     IKTTDTIIAIGASTGGTEALRELLEVLPPNTPPIIMTQHMPEQFTKSFANRLNDLCQI
                     HVKEAREGDSVMPGQALLAPGNYHMELRRSGARYYVSLNQEPQVNRFRPSVDVMFRSV
                     ARYAGGNSLGVILTGMGSDGAVGMFEMKQAGAYNFAQDEASCVVFGMPKEAIKAGGVD
                     KILPLNDIPSAMLAHLKMLAPR"
BASE COUNT        11306 a        12057 c         9358 g        11076 t
ORIGIN      
        1 aaagaggcgc tgtcagaaag tgttgaaatc ataatgggtt aaccccaact ttcttttagg
       61 gagaaaccgg gacgaagccc cggtttctcc ttaatactga ttactgtaac aaggcaaggg
      121 cttgttgagg caacgtattg gcttgagcca aaattgacgt acccgcctga accaatatct
      181 gattccgaac aaacttcgct gattcaaagg caatatccgc atcccgaatc cgtgattctg
      241 cggcggtgat attctcaccg gctacttgca aattccccac agccgcatcg atcctgtttt
      301 gcaaagaccc gatattcgct cgagcagacg ccacagcact aatggcacta tcaatattag
      361 acagtgcgct taaggcattg ccagaagtag acacattgac actgcccaga cttaatgtcg
      421 tggtgttcaa atccttaagg gacagtgata acgtatcatt cgctgaacta cgaaaaccga
      481 tttggatgct taaggagtca ttagcggttc cacttaacag tttcactccg ttaaactcgg
      541 tcaccgagga gattcgatcg atttcagacc gaagggccac aaactcctga tcaaggaagg
      601 accgctcatt cgatccaagg attccactgg ctgattgttc ggcaagttca cgaagccgac
      661 ctaacagatt accgatacct tccgcgccac cgtctgcaat ctgcaataag ctaatgccgt
      721 catttgagtt tctgacggct tgattaatgc tccgaatttg cgcccgtaag gtttcagaaa
      781 ttcctaatcc cgccgcatca tccgccgccc gggtgattcg caaacccgaa gaaagacggg
      841 cgaccgaccc ttccaattga gcactggaca cgcccaagtt tcgttgagcg ttaagtgatg
      901 cgatgttgtt gttaacgaca agtgccatac tttcctcctt gatggctgcg cctcaatgga
      961 tccgtccctt gaggaatgaa ataaatgagt tgagcctctt ggcctcctcg aaagttttga
     1021 agtgaaccaa tttccacttc tgtcgtatgt atcgactacc agaagcaaaa cttgagggag
     1081 ggattaatgc cgatcactcg gtcgctctgg cagaatccca ttacagaccc tggcagcaat
     1141 tgaacgggta aatagaaaaa gagcaacttc atatcttcca gtgggaaccc catagcaaac
     1201 tacgggaaac agaacggttc acacgcacca cagagaaggg ccagaccaaa aactgcttgc
     1261 cgttacatgg gagaaggatt tttttggaca agacgtggaa tgacctcata ctccaacatg
     1321 tccgcaattc gaacctcatc ttcgacttcc cgagcattca ataattcgtt cacccaaaac
     1381 gataaatctc ttgtagcctc gggattttta agccccacaa tttcatgaat cttcagccac
     1441 tccaggtagt ccgcaatttg accaaaccat ttgtctaagt ggtgaaactt tccatcccct
     1501 tgtcggaaag catccacaaa cttctgccct tcatcgcata gctgtgctcc gaattgtgtt
     1561 cccgtttctt cagaataccg cacgatggtt tcaacatgtt tgctcacggc cgcgatagag
     1621 ccaaacgaac tcgccacctg agccagggtg ggaggcacta actcccgatc cgtcattttc
     1681 cgatcaccaa cccttaattc cgttaccaaa caccctttgg cctgagcgcg gtcactcaaa
     1741 ttggccaaaa cttcccctag ctgaacacga tcactcactt cccagatttc ttcatccaat
     1801 tgcacatgca tagagagccc tccttggatc atgagaatac atttctttca accgcctata
     1861 attttcttcc ccacgggtgc gaaatacatg atatgctcga acatgttcct cagccatgcc
     1921 gctgagggtc gcggactcca agcttaacgt atctcgataa aaagccattg ctaacgggcc
     1981 aaaagccaag gacggcctgg ccaccagttg cattgtgagt gtgtcaacac ttaattgatg
     2041 ttgttgcgcc ttcaacctat taactggaat gggcctttga cgacacatat ctaataccgt
     2101 ctcggcttga atacacaact gatcgagctg tggagccaaa cgacgccata cctgctcgca
     2161 ttcttggaaa tccggtggag aagttggggg aaaatggacc aggctcgagg taccggtatt
     2221 aaattcatac caatgttggc gccaatactc cgcataccag gaggccgctt gttcttccgt
     2281 tcccgctctt atcgtaaatg tccctaactg atcttcattg acgccaccct catagacccg
     2341 aatcttggac gaaaacgaag gaaagggacg agtacccata agatgtaaac agagatcggc
     2401 catctcttga ggaatgatct ggtctttaca agcatggtga ggacagacct tatcgaaccc
     2461 acatgggcca cacaggatct cgggctgaac cacccaatgc cctggcccaa aaggtccggt
     2521 ttcccaaaag ttcacatgac ctactgacac gttaagaacc ggcgtattca ctcccacggc
     2581 catgtgcata ggccctgtat cattggtcac catcaactga caatgttcaa gcaatgccac
     2641 cagttgcggc actgtagttt ggccgaccgc ttcgcaaagg gttcccaacc ctcccgcctg
     2701 ccggtaacac cgaatagcct cctgaacatc cgtctcttct ttcttggtcc cgatcaagag
     2761 aaatccgact ggctgttttt gactaagact cgccatcatt tgtccgtaat attccggtcg
     2821 ccaggccttc atcggatcac tggcgccgac ttgaatggca atccatttat ccgccgtgcc
     2881 tgcttgtctc aaataggtcc gtgcccattc tttcatgtcc tgtggcacat gtagacgaag
     2941 aggatggaac gatccagttc cactgccacc aagtgaaaaa agatcaacaa tattgaaccg
     3001 attcaattgc cgatacactt ggaaatccac gaaatatttc atccaaggat ttttcaccac
     3061 gaaactccca tcctgggtcg tggtcattcc tcgctcatct gcacacccaa aatacttcac
     3121 cagaaacgca ctgcgacgat taaaggtgag attcacgaca cgatcgtacc caacctgcag
     3181 taacggtcgt acccaacctg caatatcctg atagagggtc accacatccc gtattcccac
     3241 gcggctctcg tccatcagca gctgaaaatc aaacatcaac acctgtcgaa ttccaggaat
     3301 aaggctagcc acatgggcaa attctttatc aacaatcaga tcaatgccca caccaggcca
     3361 ttcttcctcc aaacgtgaaa gcaagggaac catctgaatc aaatcaccca tccgggtaat
     3421 attgataatg aggatttgtt tattcattaa gaattgacca taaaagaatt ttttaccatc
     3481 agggttttaa agaaaatctc gtttttcctg tcggtattca tccatcatca aaattagtaa
     3541 ttcctcacgg ttcaaccggg cttccggccc tttcttccga atgtctttcg ccacatccac
     3601 taactccaca cgatcggtcg gaagaaactt ctgcagcatg ggaatcaatt cgggggactg
     3661 tccactccga gccactagcg attcagccgt acggtccccc tgaagaatgg ctcctagccg
     3721 atcaggactc gccataccca cggcacctaa aaacgtcctc atccgatgcc cgtacgtgtg
     3781 cgcctctaaa acgcgcttcc gggacaattc agccatggcc actcgtctgg ctggttcgtg
     3841 caaataagtt tggacggttg ggagaagctg atccggactg ttgatgaccg ccatcatcga
     3901 ttcatcaaag agcgccggaa gcaaggtgcg agcatcgacc acttggaacg ccccgcaact
     3961 ggctaattcg aatgttctgg ggttcacccc atctccctct ggatcgaatc cgtcccccgt
     4021 ataagaatgc aaattaatat tgaccgaggt ggcattgaaa atttttacac tagtgggggt
     4081 atcaatgcgt gcaccccctc tctgaagaac ctgggctaac ggcccagggt tgtcccattc
     4141 attcccccac aatttaaatg tccacccttg gccgaccagg gatgggagga tctgccgccg
     4201 gtttctatac cctgccccca aaaaagacac atcggcacca aactcgtatt gcgctccctg
     4261 ggtaaggatc attggttgat gaatggccgg atcagcagct aaggggagat acgacacctg
     4321 tttggctccg gccttcctga aagcgtccaa gcatgcggct tgttgcatca caaaccaaaa
     4381 gtcatatccc gacaccatct gctgccaata ggttagatga cgaaaatttt ctacaaacca
     4441 catggccgtt aacacctttt ttcgacgcat ctgctccaaa accgccatcg ttaatggcgc
     4501 ctgggacaag gctaaaacca ggtcaggggg atcttctgcc acatgagcca ggctaatcac
     4561 cgccaaggtt tcactcatcc gttgttggac tgtgagtcgc agccgatgat cacggattgt
     4621 gtccaaattc tgatatcccg aataatgtgg actatgatcc acccaactca cacgatgtcc
     4681 aaggccttcc aaggcgttca ccacatatcg ggcaatgggc aacgacccgc cataaatggg
     4741 accgatcacc atgatgtgaa gcgttccgct taagccttga gcgagatgat cacgaagttg
     4801 caaacggaaa tgttcataga ggtcgtgatg aaccgtggcg gcaggttcgt aactcagaat
     4861 gcgaacccgc ttggctttct gggtcacttg gacagccata tcagtgatgg aacccgtcat
     4921 ccacatcagc cgttcacccc acccttccag ggaccggacg gaaacacaat ctgcgaattc
     4981 ggaaaggtcc ggcaccacga ccatcatgtt ctggtcatga ggcaacatct gacgcaaggc
     5041 ttccacgtgg tagagcagtc ccacgccgag gaggacaatc gtttcccctg cttgacagtc
     5101 tttcacgcgt tcttcagccc agcgccctgc ttcgcgaacc ggatcatacg cactatgaag
     5161 atatcgggag ttgaccagcg cggttggcat tccggattta gcaggatgga tcgacagggt
     5221 tcctcctggc acctggagga tctgagcagc aagaatcgga tcctggcttc gaagaatacc
     5281 gatattcttt ttcaacacgt ccatttatct gttcctggtt tattaacaat gaaacacacc
     5341 cccagccttc aacctaccgg gagtatttcc tactacttcg cgacttccat ccgaatatta
     5401 tgattcgcta gctgagacag cgcctcccac gtatcttttc gctgaagaac agcctcgtca
     5461 ggaaggccat cagtggtata aaacgccccc cactcatctt tattactgat ccagagatcc
     5521 cactcgtcaa ttcttgaatt cacccgttca tcccgtatta agttgaccgt ttccatcaca
     5581 ggccaatggg caaaggatga ctcttccgtt cgtaattcac gagcatccag aacatcccca
     5641 ggtcgccctt gatcgtgttg ccacacataa tggccaaccc catagggacc ggtttcatgg
     5701 actctggcct gggagaaata ccaaccgatc gcccgtgtcc cacacatcgc tccgagatgc
     5761 aacggtcccg tatccgatcc gaccacccaa tgacatcgat taaggagagc cacaagctgg
     5821 ggcaacgagg tacgaccaca cccattcacc acccgattga gatatttagc gggaaggcga
     5881 tgttccaggg ccaatgccgc ttcccgttct ccctcgccac caatcaaaag caggtagctc
     5941 tgaggcatgt actcagcaca tgcggaaatc agggcttgcc acacagaaag gggaactcgc
     6001 cgttcggtat ctcccgcacc aagaacaatt ccgatgaggg tagaagatcg attattcacc
     6061 atccattcca gctcaagagg caatttcacc agcggggcct tcaagtaggg aatgtccgat
     6121 ggcggaaaaa cttgacacaa tccgcaaaat gcatccgcta aatgcacccg gttgctccct
     6181 cgatcttgag caatctgacg taaatatccc gcccatgcgg gaagcctctg attcagaggc
     6241 ccattttctc ccgggccgac caccagacta ctcaaaacat gggcagcaag tatccctcga
     6301 ggatggttat tcaaattata ggccaatgaa tacgtgggga aggcatgttc agccaaatag
     6361 cgcgtggctt gggctaactg ctggtcacaa tccgtcatct ttgtcctggc caattcatgc
     6421 cattgttccc cataccaagg gtaaaccctg tccacacaag gaaaaagttc tccaagcgcc
     6481 accaagggag atgggcaaag aaggtcaagg ggtcgctcag gtttggcaga ttgtagggct
     6541 gtcaaaacag gaagggattg gaccagatca cctaaccgag cgagttgaat taagagtgct
     6601 cgggacatag gctaacttga ccaggcaaga agttcctcta attatgttgc gacggcatgg
     6661 aggtctcgag gtgcggtctg gaggagaacc cctaaatcaa gtaatccctc atggtcgggc
     6721 attaagaggc gcaacatctc aaattgttgc ttggccaaat caagccgact agcacgaaaa
     6781 tgcacaccag ccaaagcaaa ccgcatatcg cagtttgcgg gattgcgttc gacataccgt
     6841 gaaagccgtt cccctaagtc tttccatcgt tgcagagatg ttccggcttg aatcaaccca
     6901 ttcatggctt caacatcatc aggatgatct gccacaacct ggtcgaaaac cctccaggct
     6961 tcttccccgt ctcccatccc catacatgcc attcccaagc cgatgttggt ttttcgggaa
     7021 tctgagccat attgaagagc gcgccgaaaa gactttctcg cttctcccca ttcctgggat
     7081 tgcatgaaca ggatcccctg taaccaatgt gctgaaccat cgttggggtc tgtttgaatg
     7141 agggcctgga catgttgagc cgcgtcggac agatctttca cgttgagtgc cagttttgct
     7201 agctgttccc ggatctctcg atcttctcca agactcagaa ggcgtgtcaa aaatgcccgc
     7261 tccccatcga ataactgcaa tgttcgacag agtttcacgg cccaccgcaa cacttctcca
     7321 tcatctggcc actcgcgaaa atcggaaaga agggcatgta attcaccatt gtcctgtttt
     7381 ttaatgcccc gaattgggtc atatcgccgg gataataaga gctcttgtac gcgttggacc
     7441 cgattccact gggtccgttc tccatcgctc cgaaatggtt cgagcgactg ccgaagccac
     7501 ccgtcacgaa aataatagcg attggcatag ccttccggaa cggtatatac gtcttcatcc
     7561 actacaattt ttgaacccca gcggctcatc aggcgattcc cgttatgacg ttctgcttcc
     7621 ggatctttgc gaccgggagt ttgatgttcc agatgataca atacactctt gggttggtag
     7681 ataatttttt taccgcgctc tcggattttc aaacacaaat caacatcctc aaacccgttt
     7741 cggaattcct catcaaatcc cccgattgac tcaaaatctt ctctgcgaat cagaaaacag
     7801 gccgccgtca ctgcttgaaa ttcacggcga acattggctg catgaagacc cgcaggggct
     7861 ccactgaaaa tatgataggg agtcaggcag tttttagaaa aaaccactcc ggcatgttga
     7921 atggtgtcat tcggatacaa cagtttactg ccgacaatgg aaacatcatc gtgcgtctca
     7981 acttcctcca acaaagctgt caaccatcct gattgtggaa tagtgtcatt gttcaggaag
     8041 attaaatact ttcctcgtgc ggcacgagcc ccttgattac aggccttcgc aaatcctaga
     8101 ttttcagtat tccgaattat ctgaatatcc cccccgaatg aagccagaaa attctgagtc
     8161 tcatccgtcg acgcattatc caccacaaga acctcatagt ggcatccttg tgtcacttcg
     8221 gccaaatgca acaggcattg cttcgttaaa tcatcacgat tacacacggg aataataata
     8281 gaacactcat attgtgtatc aacaattggc atgtaggaag tcggagttga tcgagcctcg
     8341 ttattcccca atacggaaaa gtttgaatga tcccgcctgt tagcagattc acccagccat
     8401 acggctcgat attgtggtag ccaggattcc aacattcgat gccttaggac ccactccctt
     8461 aaaactttgc cctctttctc agaatgatcc gaattataaa gatgatcccg aattgctcgt
     8521 acccaggctt gaggatcatt aggaactcgc gtgaccggtg cagttcgatg aggataaata
     8581 tcagtggcaa tgaccggaaa tcctaataca ccatattgca aaagacgaag gtcacttttt
     8641 gcctcattaa attcatttat ggctaatgga gctaaagcca gatcaagatt cagactggca
     8701 agtttggatg gaaattgacc taaaggaacc atgggatgga cttcagatct gaccccggtc
     8761 cgtcgaagat cttccggcac ctctcctaaa aatacccact caacctcatc acctaactcc
     8821 tcgaacaccg aaccaaggaa gcggagatca gaacggtgta ccccaacctg tccagcccaa
     8881 ccaatacgtg gcctggagcc accccctcgg agaggagcca aatccctcca ctcttcggag
     8941 accaagcaat tcggcagaag ggcagccttg attcctacac tttccaaggc ggtttgtaag
     9001 ggaatggtag aagttgttac gcaatcggac aattcaagca atttcatcaa atggtcaata
     9061 agccatttct tcatcacttg atgattttga ttatcttccg ggatcttcca gagcaaatca
     9121 tcgatatcgt ggaccaaacg ggtaccattc gatcgcgcaa taaccatatc agcgatttca
     9181 atcctacacg ttcgctgaac aatccacacc gttcctggac cacttggtaa ttggtgagga
     9241 tggctcccct ccgctccttc aattttcacc attccgccaa accaggattt cattacgctc
     9301 gcggtgagtg cttggagtat tcggatttgg ctacatatgg gatcattaat aattgccaca
     9361 acgcgtggag ccacctgaag gttttgacct gaggtggaaa tcctatcatc caaacacatt
     9421 tttggctctg ataggcttct gttgagttcc ttctcagaca agactcgatt aatgcggatg
     9481 aactgcttat ctaaatcctt taccgtttgc gtaagatccg caatttgtcg ggttttttcc
     9541 aatagggcct gttgaaggat aatgttctgt ccatgaactt cagcaccata accatcctga
     9601 ttcgactgcg tatgggatac acggaactgc atcgcggaca tcattccgta actgagatta
     9661 caacccccta cgacaagccg ctgaaattca cattccagat cgtgatagtg cttaatgccg
     9721 tgatcggtaa acgaaaagcc ggccccttgt ccaatatcct tgataagaga aagaatcgaa
     9781 ggaagtgtct tttctcccgt tggcgctatc atgcctaaag cataaaatga agagaacaaa
     9841 gcacgaaaga cgacataacc aacctccagg tcttcgccaa gcacccactc ttgatcaaaa
     9901 aatgtcacct caccatttcc ttccatgatc atattacgtg gaataagatc aaggaattgg
     9961 cctgatacct tcttcttagt catatcgttt gacttctgga tacccacaac gtcgaaaaac
    10021 cccttcagcc agacctttaa ccattcttct atttgcgtaa cccgccaccc agggctggtc
    10081 atgatttgta caagccgatc ttgccaaagt tcgcctttca caaatgtttg atcaacaagc
    10141 cgttgggtaa ccaatgaatt cttatttggg gtgtcagtag ggtacagccg cacttgagag
    10201 gtataaccgg cacgctgact ctcctgtgta aaaacgacct ttttggcaaa ttctggcctt
    10261 ctcccagttg cataatgtac cgcgagagga acaattgatt tttccttaca agcgaggtct
    10321 gtggccagta atacaaagga attcgccaaa tcaggcaaaa gaccattacg aacaattggg
    10381 cgcaaagctc gttcctgatt aaaatgaata cgagctggaa attgcgggtc gtcggtgcac
    10441 gcactacgca ggaccggggt cagatcaata ccgtcctgcg gcatggcccc gcgttccgac
    10501 accatcaagc ttggtagctt gtaatccgga aagggatacc accattcctg ttcaggtagg
    10561 ccagcctgac cgactcttcg gctaagctct ctccggccaa aggtcacaat gctccctttg
    10621 gtgtaatgtt cttcgatgcc aaacatgggc ttgcccatat ggtcttcggg aaatccggca
    10681 aaatatttca accctagctg gttttcaata gcgataatca atgtacctcc aggctttagc
    10741 aaacccttca taaaaaccaa catagcatca actgggtctg ccccatcacc tgggaaaaat
    10801 ttcctggcat attctaaaac tccaataacg gtcacgacat caaactgtgg tccaggatta
    10861 aaatgatgaa caacttcccc cataactgtg acattgctca ggccacggca acgcaatgac
    10921 gcaatcgatg cgcgacggag gctcccttcc agtgccacca cctccgctcc tatttcaccg
    10981 agataacgcg tgagcgcacc gcaacccgca ccgatttcaa gtacactttt cccttgtaat
    11041 tgttcctcaa agggccgaag cagattgacg cgtttccgtg ataaatgata cagcgaatgc
    11101 caatccgtac aggattttgc caattcgccg gacatcacac tgacatcgct cgcaccggaa
    11161 ataatcgttt tcaatcgatt ttcaatttgc tcgccatcac tataattgat gcctttatat
    11221 ccagggcgat gccacactcc ctcatcttta ttgaaaagat aaccggcgct tgtgaaatag
    11281 gatttcatag gcgaacttgt actcctaata ttctaagaat gagccattga tgtgccaaat
    11341 tttggatgaa ttctaaggta gaaaaccatc caagcgaatt cgcatatgag ggttttcaac
    11401 actccccaaa agatcatttc aaattaatat gagttcaagt tttatgccat acagttcaag
    11461 atcaaaaggc aaggtttcag gtcacaatga agccgattcc cacatcactc gaaaaaattc
    11521 taccgactag gaattccttg ctaataaacc gggaaatttc tgcctggaaa ggaaaggatg
    11581 aaggcaagac ttgagaaggc actgaaggat tatggagctt ccgcttttat taccacttcg
    11641 cagcagggct cccaatgttg gaacttcaac cctacctttt gaaaagaggg aaacagccct
    11701 attgaccgag gctacgtgtt ttattccttc gcccaaacgg gaaagtttgt cattccagtc
    11761 aattctcaca aggacctgta gggaccatgg gattgtttac caggaatgcc cctgaactgg
    11821 agcctcttcc cgttaggttg attcggtagg tttagcctga ggtcttttct gtctgggtag
    11881 gctggccaaa agcttcggat ccgaggatac ggcattcaaa ttttcctgcc ggacggcttc
    11941 atataattcc tcacgaagaa cacggacatg cgacggacat tcaatcccga gtcgcacctg
    12001 tcctccttta atttggacaa ggacaatgcg aatgtcatct cctaatcgaa tagcttcatc
    12061 aatttttctg gtgagtatga gcatggtgcc ttccttggct ttggggtaaa agtcacatgt
    12121 aaaacaatgg catgtacatc atcaacgaat tacatctgga cgatcttgct tcttcggagg
    12181 cgtgactgca tagtttacac cgatgcagcc tctgtcaccc cttcagggtg gggttgacct
    12241 tccgccacat catggaataa aggatggcgc aaggagattg attcatggag aatcaattgt
    12301 ttcccttgtc tggttcgccc atttacgacc agaggggcac gaagatttgc tgtcgcctta
    12361 tctggatgat ctgaagggat cgagataacg gccataattg aaattggatc ggcaggagtc
    12421 atatccaatt cagccaatcc ttctccggat aggtttgtac gaaaatcggg ttgcaacaaa
    12481 tccacatcca taatcacaaa agctaaactg ggttcatcca ccgactgaaa ccactgatag
    12541 tcggcatcct ctggcgggtc aagtacgaga taccgtcgga aatctgggaa acctacgagg
    12601 ccggatggga aagttaacag cgtgtcatcc gacacatcca acataccgaa ccgactagtc
    12661 tgaactttca tgatggtatg ttctccaaat agttcaatgg tttcttcctc atgccaaggc
    12721 aaccccgcct cggggtcggt ttcatttttt ctgtgattag gacaccctct ccgtaccaca
    12781 gattttgacc gaattgtcat ctctcaagac aatctgacag gcatgagcca attcgtcgac
    12841 atcatacggt ttttgaataa accctacgac accctgattg agtaaatctt gcacagcatg
    12901 gttccgatca taccccgtac agagaattac cttgagatct ggtcgaatcg ctcgcaattc
    12961 ttcaaaacat gtcgctcccg acatttcagg catcaccata tccaacagca caacatcaac
    13021 gggaacagtc aaatcccggc agattttcag ggcctccttc ccattcaaca caaccgtcac
    13081 ctgatagcca aggaattcaa gtatggcctg agcggcttct cctacatcct tctcatcatc
    13141 aatcactaca atatgcccgg ttccgtgaga aggctttgtc gtctgaacgg gggcgtcctt
    13201 aaggctcgca catagggcat ccggaagata tacacgcatt gtcgttccac acccaggaga
    13261 actggtcacc ccgatatacc cgcgatggtt tttgacgatt ccatacacca tcgccaatcc
    13321 cattcctgtt ccctgtcctc ggcctttcgt cgtaaagaat ggctcaaaaa tatgtcgttg
    13381 ggtctctaat gacattccgg tcccggtatc cgtaacccga attaccacat aatcacctgc
    13441 gtgaagtcct gggacacgtg aagcgtattc ctgggtaacc gtttcattcg aagtctgcac
    13501 aagcaattcc ccacgctcac ccatcgcatc acacgcattg acacctaaat tcatgagaat
    13561 ctggtataac tgattgggat cccccaacac ataaggcttc gtagcggaca ggtcagttcg
    13621 gaaggtgatc attttatgga ttgtccttcc gagcagtgtc atgacctcct gaattacatc
    13681 atgggtatcg accgtcacat catgatgctt tccctgcctg gcatacccca agagctggga
    13741 tgtcaattcc tttccacgac gaacggcttt ttcgatgaca tcgcccgctg cccacaaggg
    13801 ggtgtcagtc ttttcctgta tcttgaacaa acttgcatgg gccatgattg ccgttaacgt
    13861 attattcata tcgtgggcaa cccctgaggc tagtcgaccg atggcttcca ttttttgaac
    13921 ctgccgcaac tgatgctcaa gggcaatatt ctcttcctcc atgcgctttc ttgccgtcaa
    13981 atcgcgtcca ataaaaagag tttgacgctg gcctaaaaac tgaacctccc gttccatcat
    14041 ctctacggga agattgctgc cgttcttttg acgaaggacc gtttcctccc atctcgcccc
    14101 cccaggggca gagcttggca acgtgggacc ggattcccca cttgatggct caggtctgat
    14161 aagagagaag gccttcccat tcaagacaga cgggagatat cccaattgct gcgctccaag
    14221 cgaattaaca tgggtgattt ttccctggaa atcataaatc accaagacat ccggaagcgt
    14281 ttcgaacaac tctttaaatt gggcttcaga ttcgcgtaaa tcatcagtac ggatatccag
    14341 taaatgctct actcgtgaga gtgcccgctc ccagcgccct ttttcacata gtgaaataat
    14401 atgcaatgga tcgcacgctt ttgggagata ggcatcagca tgaatcgaat gagcagggta
    14461 tccacgggct gtagattcct gtccaaggac aatgcacatt gcctgaggat attggacttt
    14521 gcaccaaatt acaaactctt cgcttgtcat gtccgacagc ggatggtcaa tgaggacaat
    14581 atcaggaagc acaagattca ctgccacctg aacctgtttc cccgcttgcc attccaccac
    14641 ctgccatccc cgttcaccaa atatgtgatg aattcgttgg atttcggact tgtgggtgga
    14701 aaccatccaa acatgtgggc gttgaggact aggagggttc tccactaact cgcaaacctg
    14761 ctgacgaaag cgttgaggtt ctgtcgggag agcaagaaat gccgaagccc ctaaatcttt
    14821 caaaatgtca tgagcatcaa ttccagtata cgtggcggaa accgcaagaa cagggggcac
    14881 tgcctgtgtg gaaccaaaga atgaatgcaa caattcgcaa aatcgccatc cgctaattcc
    14941 cggcatatgg agatcgagga caatggcatc cggcacattt ccttcttgga gccaccgcca
    15001 ggcatcttca gaacagacaa agcgattgac cgcaaagtaa tccttctcaa gccaactcgc
    15061 aagaagatgg agttggaccg gatcatcatt aattacaagg atggacgcca tatttattgt
    15121 ttcaatgaac tcggcgaaat cgtgagccgc gaaaaggtat cgtccgcatg cctatgaaat
    15181 tgcgcaaatt ccaccccatt tcgaccgcta tttttgacta agtagagaac accgtctgca
    15241 gattgtatca attgctcaat gggaattgga cagggatcgg tccaggtcgt gaccccctga
    15301 ctgaccgtca ccgttaaatc aacattgtga actcgaaagg gtttctggga aaccgccact
    15361 cgaatgcgtt cagcgagccg cacggcctgg gccgcatcac tattgggcaa taccatgaga
    15421 aattcctccc cgccataccg gccaatggca tcatatgaac gcaagcaaaa ccgcatccgc
    15481 ctagagacct cgaccagcac gtgatcaccc gcaacatgcc cataggaatc attcacattc
    15541 ttaaacccat ccaaatcagc cagaatgaga ctcaggggtt gctcttcccg atgagcccgg
    15601 tcaatttccc ggagtaaggt atccacgatg gcgccgtgat tcagaatttg cgtcagggga
    15661 tcttgcatcg cctgaatccg aagtgtttct cgagccgaaa ttaattcatt ttgcaaatcc
    15721 acaattcgct tccctgctcg taatcgagca tccaattctt ctaagaggaa tggcttggtc
    15781 aggtaatcat cagctccagc atccaacccg gcaatgagat cctcaaggtt tttccgccct
    15841 gttaacataa caatatatac ataagggcaa tcagttctcc cccggatttt ttgacacagt
    15901 tcaggaccgt tgagcccggg catgatccag tccacaatag ccacattggg cacgcttcct
    15961 cgttccaaca ccttccacgc ggaatcccca tcagtacagg atatgacatc atgcccccat
    16021 tgacgcaatc gatagacaac catatggagt gtgagtggat catcatcaac aacaagaatt
    16081 cgcatagaga tgtcgtgcct atttttttaa aacggataac gtatttagtt caatggaaaa
    16141 aggccgagtt catgccctat tttatgaagt tcaacaagct cgtctgaaat tgacgcgcca
    16201 gggtgctcat cgaagcttca agggtgtttt gcaatgtagc caaatcagag gccacttgag
    16261 cgatatcggc atcctcaaaa ttggaccgga gcgtttgcgt attcacgctc aacagttcca
    16321 atccatcctt caccgtatcc aaacgattga ctctggcccc gattgtagcc cgggcgtcgg
    16381 tgacttggga gtgggactga ttcaattgtc cgatggcggc ttgaataccc ttgccatcgt
    16441 tcgtcaccag ggcttgatga aagtcctgaa gatcctgaaa tagatccacc gtgggcccgg
    16501 aaaaaatttg gtttcccgga acattggttt ccacggtccg gccatcacca atttctacct
    16561 cgatccccgc tccatctcct tgataggtat agccaactct tacaaaaaat tgatcgccag
    16621 cggcaggcgg cccgcctccg tcagtaatgg tggcatccaa tccttcaaac gagaagaccg
    16681 cgccggatga ataggtatta cctgatgaga cggtttgatt cgtggtcaga ttcaggacat
    16741 caaactgggt tgaggaagta aattggattt gatacgcatc cggttgtaag gccgacgccg
    16801 tgacgacaga aacagcaata tttgcgcttc ctccattgtt gatgctagct gatcccgttg
    16861 ctgatcccgg cacaaatggt tcggattggg tttgacttcc cgcaaacaca aatcttccgt
    16921 tgagttgagt gttggcaata ccggccaacc ctaggatgat ttgttgaact tccagagcgg
    16981 cattccgtcg ctccaccggc gtattggtat cgctcgccat attcaatgct agttctattg
    17041 cccgctgcac cgtacttcca gccgtcccca gaacgctatc ggataaattg agccttccca
    17101 ctccttcatt cactgtggtc aaacgttgtt cagtggtccg taggacattt cgaaactgat
    17161 tgatcctttc cgatgccgca ggattatccg aggggcgatt gacccgcttc cctgaagaaa
    17221 cctgctcatt gcgatccccg atatttccct gaatacgttg aatcgccctt agcagagcat
    17281 caacctgttg acgatcagtc acccgcattc gattgttcgt ccttttccga tagacatcga
    17341 tttgtcatcg atgagtccta tcccagccag ttgcctcatc aagaaggtta gcgacccatg
    17401 gacagcatgg tttgcatcaa ttcatccgcc accgtaatca tccgggccga ggcttcgaat
    17461 gaacgctgga aactgagcag attagtcagt tcctcatcca atgaaacacc agagacactt
    17521 tctcgcaagg ccgtcacttg atcgacttcg agctgtttac tatgtaacgc ctgtgtcgat
    17581 ttctgcgtgg cactcccgac atctccaatc gtaatggtgt gataatcatt gagtgtcaca
    17641 ttacccagat ccagttgccg gttcgtatga acagccacca tatccaatgc attggtgttg
    17701 ttccccggta ctcctgcggc cgtagaagaa gccgcaatct tgtccgtatc cgacagggcc
    17761 acaccaaatt gcagtgcggc gcctttttga gcattcacat aaaatacatc gcccgcagcg
    17821 ggtgtgccgt taatcacgac atctaatcca tcaaaactca atggagctcc ggaggtatag
    17881 gaaccggagg tcacgctcgc tccggttgcc gtattgacca ccgaatagga agtggctccg
    17941 gaaaactgca cctcatatcc ctgaaacgtt gcaagagtag aatctgcaat cgttaccgaa
    18001 ttgccggtgg cgcctcccgt attccgctca ttggccaaag gggctggggg agttaacgtg
    18061 gaaaagaaat tattattcgt ggtggcgtcc aacccatacc caacctgatg ctgttgatta
    18121 aattcggtag ttaaaacggc cgccaatcga tcgacttggt cctgaaactt cacaatatcc
    18181 ccatcacgca gagcgagtaa tccgccgatt tgtcctccat aaatcttatc ggaaattgaa
    18241 tactcactcc cgtcactgcg cacgaacgtc acatcatgga gcggggggtt gtcggggtcg
    18301 gatttagtcg acagcttatt ggcatgattt cctcctacca acaattgtcc acctacatga
    18361 attccgatac catctttcat ggttacttgc tcaatatcca ctagccccgc caaatcatta
    18421 atgaggaccc gtcgttgatc ccgtaaatca agggcttccg gtccactgga ttctacttta
    18481 aaaataacat cattcagcga ggcgattttg gttgccaacc cattaatagt attgacatgc
    18541 ctggaaattt ccccattggc atccaggcgg atttgattta accctttagc caccgtccca
    18601 aactggtcgg tgagggattg ccctttggcc aacagcaccg tccgctgaat agtactctct
    18661 ggattcgtgg caacatcccg aaccgcattg aaaaattccg tcagactcaa cgatagtccg
    18721 gaattatccg tttcggtgaa aatcccatcg gcctgaacca ggtaattgtt cctggcggaa
    18781 atagatccca gatcctcatg gagggcggtc aattggtttt ccaggaaggt atcgacggtc
    18841 tgacgaatag cagcaactcg gactccgctc ccaattttcc cttcagcggg ccgcgctgac
    18901 tcgaggacaa cttcctgtct ggaatagcct ttggtattga tattggcgat gttatgggat
    18961 gtggtcgcga gcccctgttg gttggcacga attcccgaac gcgcgatatt aaagatatca
    19021 ctaatccccg ccataagagt cctatccctg aagatagacc gagctaggaa ccgatggaaa
    19081 atataatgtt ccagatccct gatacaccgg acctgtaccc aaccctgtat acatcacttg
    19141 aatcgcctca cagactctgt gatagatcgg ccgaattgca gattcatttt ttttgccttg
    19201 ctcctgaata agccgcgcca aacggcgaag gtcaatcaac atgcgttgca ccgtctgtgc
    19261 ctgtggatcc ggatttgcct tcaaccaggt ccaaatggtt tcttgggcga cgtccccgac
    19321 caactgatgc aaggcaccct ccacctgctt ttctaatcgg cgcatcgaat tcaaaacttg
    19381 ttctttttgg ctgttcactt cgaccaactt gggctgatcc atgatgcgta ggctatgggc
    19441 ttcttgaaaa agcagtacac catactgctg aaaagccgcc tgagcctcgg ctaatgcctt
    19501 cagtagtcgc tcccacttcg ccgattgagg gtgattcatc ggattgcctt tcccctcatg
    19561 aacttccgat aattattttg ttcccaattc gttcagtatc gtctcccggg tcaaggcatc
    19621 agcgacctga ttggaggtcg ctaattgggt ccccgtttcc aaggcctgct gaatgcggtt
    19681 gattttgtct tctctcacct caggaaactg cgccactcga atcgaatagt cacgaatttc
    19741 ctgtgccagg acggaaagat cgacagggtc agcagacgaa gggtgctggg aggcccgcga
    19801 cgggccagcc tcctgggtct gaagattttt agcttgaacg tgaaaaaata gtttccccag
    19861 ctctccactg ggatcaaggc cccgtatggt catggcatct tgcctccttc tctatatgta
    19921 tcggaagcaa caccattaaa ctttacagaa cctcttatga tttcaaaaaa gggggacctg
    19981 atgagggaga acgcttgaga tcttcattcc tggccaccgt gtccaagaca taggccgaaa
    20041 gaccaatccc acccgattga gcggcacgct tgccaatttc ttcgtcataa aatgaataat
    20101 acatctcccc catggggttg gacgttaatt cgcctttggg gaccgtctct cgcatcacct
    20161 tcatcaaata ggacaggaaa taggattcga actcctggct ggcgcgcaat aattcccctc
    20221 gacgagccga ttctgccacg cgatctattt tgccgctaga tacagaatgg gagagacccg
    20281 tcatatccaa aggaaacatg tcaggaatca tggtctttat cctttccaac agaccgtcat
    20341 gaataccccg ctgccttctg taccgaaatt agatcaattc caagtctgct tggagcgctc
    20401 ctgccgcctt ggccgccgtt agcacagcaa ccaaatccag aggagtcaca cccaatgaat
    20461 tcaatgcctt cactaaatct ccaagcgtca cagaactatc gacttgaatc acccgaccct
    20521 cctcttcttg aacatcggtt tgtacatcag gggtcaccac ggtcctcccg gcaccctgcg
    20581 ctccgcgacc aatgaaggca gtctcaggct gagagacatt aaactgcgtt ccaaccttga
    20641 tggtcagatc gccatgagag atggccatac tcgccaaccg gacatgttcc cccatgacaa
    20701 tcgttcccgt tctttcattc accaccacct tagccaggac atccacatgc acatctaacc
    20761 cttcaaccgc agcaatcatt tggacaatct taccctgaaa ccgttcggga acccccactt
    20821 gaatctgtcc ggaattgagc gccgaggcga ccccatcgcc aagatgcgca ttgatggcct
    20881 cagacactcg caatgccgtc acgaaatcgg cctggtgcat agtcaatgaa aatttctccc
    20941 acgcctgcat atccaaatct accgatcgtt caacaatcgc tccaccggga ataattccac
    21001 cggattgttg attcttcttc actgtggttc ccccagcccc ggcttcaaac cccgaagtgg
    21061 agaggggccc ttgtgcaacg gcgtacacct gttgattggc cgctttgagt ggggtcagca
    21121 acaaggtgcc tcctcgcaag cttttggcat tggccatcga agagacctgg acatccaatt
    21181 tcatgcccgg tcttgcgaag ggcggtaatt tggcagtcac catcaccgac gcggtatttt
    21241 tagttagcaa ttgaatggga tcgatattga gtttaagtcc catggtattc aacatggaaa
    21301 taatggcttg cgcggtaaac tgaccaccga cgacggaatc accggtccgg tccaaaccaa
    21361 caattaagcc gtacccaacc aactggtttt cccgcacgcc ttcgatagag gcaatatctt
    21421 taatccgggc agcctgagtt tccgccggtt cggtcaaaga cccagtacca gctatgagcc
    21481 atccactaca gcaaaccaag accaaacccc atcgggaaaa ccatgttcgc gtgaattttt
    21541 gtaattttgc gtggcttttc ataaaggacg atggatgaat cacaaaactc tcccgttggg
    21601 tcaaagtaag aaaaattaat tcagctcaaa accctaaaac ggcgtaatcc agtcaaaaat
    21661 tctggtggcc catcccggtc tctgaacatc gtccaccacc cctagtccgg tataggaaat
    21721 ttctgcatca gcgaccttgg atgacaagac catgttctcg gtatccagat cgatccggcg
    21781 gacaatgcct tttatggtca ttgtttgcgt ttcactatta accgttacct cccgttttcc
    21841 acgaattcgt aggtccccgt tgggaaagac ttcaatcaca ttggcggcaa tggtgcccgt
    21901 cagagtatct tcccggctgg tcgacccgtc gccctcaaat tcatgggagg tcccagcatc
    21961 aatattgaaa tattccgcaa acttctgggt gagggtgtta atcccgaaga gtcccccact
    22021 tacgccagct gataggctgg aatctcggtc tgctttcgtg ttggcttttt tggaacctcg
    22081 gtgttgttcc acaatttgca caacaacgat gtccccgatg cgaccggctc gtcgatcctc
    22141 aaacatgaag gcccgcccgt tttcgggatc ccacaagcta cccagggagg atggtgtatc
    22201 ctttttatgg atgttcggct cggcttcgat ttgtgttatt gttttttctt tggcacagcc
    22261 cgtccaacca ctcacaatta tcagtagagc gaatcctccc gctccgaatc tggttattaa
    22321 ccgtgtaaca ctcaaaatcc cacctccact agccccgatg ccattaccgt acctaacacc
    22381 tcccgaccag acgtctggtt ttttacggga atggtttccc cccttttccc agccgatttg
    22441 gctactccaa cggtctgaac cagcaggcct tcgcccctga cctcaatcat cactcgatca
    22501 cccttatgaa tcattggtgg gtcgtccaac attgttttcc gaatcggttg gcttggaggc
    22561 aatggacgta agacctgttt ccctacgact tcgtcaagat caagaataaa atcatgcgtc
    22621 agtgaaggtg catcgaccat cattgatgac aggtcctcga ccgtcacgac ctctgtcggt
    22681 ttcaaccagc gaattggagt cgccacttcg acttgggcct tcacctcacc cacaacattc
    22741 acggtttgga gaaactgctg attgacaaag atccctactc gaaaggcacg tcgcccagtc
    22801 cttccgcctc ctgaccatgc tccgatttca agatggagtt ttcctttggg tacctccacc
    22861 aattgtttgg ggaacaatac ccgaaacgac acctgatgat gcggacgtcc atatcgacgt
    22921 gtcagttcag acacgattac ccgctcgaaa tctttgacac ctaccgtttg cgtgttcaga
    22981 cggactgggg tcggtgagac cagctcttcc gcccaagcca ttccttcggc tgtccaccac
    23041 ccatgcccgc acagaagcaa tcccaataat aaaatgcttg gcatcaaatt cttatcctct
    23101 aacgcctgag tccattcaca acttgcatca tttcgtccga tgcctgaatg gctttcgcat
    23161 tcagttcata ggcacgttga gcaatgatca tattgaccat ttcatccgca atattcacat
    23221 tggatatttc aagaaatcct tgctggatat ttccaaatcc cgtggaaaat cccggtgtcc
    23281 cctgctgagc aggacctgaa gacgaacttt ctgaaaataa attattgcct tgggcaatca
    23341 atcctgacgg attatcaaat cgtaccaatt gaatttgccc gacctgcgtg gcttgagata
    23401 ctcctggtaa taacgcggat acagttccat cctgcccaat atcgacttgg agtgctccgg
    23461 acggaatagt aatgctcgga atcagctgat cgccatctcc cgataccacg ttgcccgtat
    23521 tatcttgttt gaaggatccc gcccgcgtat acatcgtagt cccatccgga cgtgtaactt
    23581 ggaaaaaccc aaccccttcg attgccagat ccaacggatt ggtcgtttgc ttgaggcttc
    23641 cttgaacata ttccttggac acactgacgg gtctcacgcc tagcccgacc tgaatgccaa
    23701 ccggaaacac cccaacattc gaagcgctgg cgccagggag ccgagcaatc tgagacaaaa
    23761 gatccgcgaa ttcagctcga cttcgcttga atccggtcgt attcacgttg gcgagattat
    23821 tggcgatcgt gtcaatattg agttgttggg cagacatgcc cgttgaagca gtatgcatcg
    23881 ctcgaatcat tctggtctcc ctttttgtac tgtgccttaa tcgccggggc atggtcagac
    23941 agcaataccg tcagatggct ttcaggatgc atgtgaatca tcatccttga acccgacctg
    24001 cttgaatagc ttgggccgcc agttcgtcca tcgctttaat cgccttctgc atttgttcat
    24061 attctctagt cactttaatc aactcaacca tatccaaagc cggattcacg ttggattgtt
    24121 ccaacatgcc ttggtgcacc gtggtctgct tatcggcgac aacctgatct ggcacggtcc
    24181 agaataaatt gcccaccttg accgtttctt gtccatccgc aattgtatcc acccgcaact
    24241 tgtccacaac ctgattattg accctaagag tcccatcgac cccgatcaca acatccccag
    24301 gcggaagagt aatagcccct ttctttccta ggagggggtc accggtatgc gccactaagt
    24361 taccgttcgc atttcgatat aatttcccac cacggtaata gcgcaatcca tccggcgttt
    24421 gagcaaccaa aaacccattg ccctccaacc ctacatctaa cggttctccc gtctgaaaca
    24481 aagcgccttg ggaacgatct ggcggaagcc cgtccattgt gggaaataaa tctattcccg
    24541 ccactgacgg tcctgaggtc tgccccagga tggtggcgaa aaccggctta tcctttttaa
    24601 acccggtggt atgcacattc ccaatattat gagagagaac ttccaggcgg tgttcaagtg
    24661 taatcgcccc ggctaagggg ggatagatac ctcggttcat tacttcctcc ttgaggtgaa
    24721 tacctgaaga attctgcaac gcatgtgcca ataaggaaag gacagatgtt ttgaaccatg
    24781 aagagggtcg gaagatagaa ttttccaggg aagagaacct ttcactccac aaacctggca
    24841 ccagtgcagc caaattggct atggctgcct gatgatccgt tcttgtctaa aaccgagagc
    24901 cgttgtaacc acgggaagcg caatggccca taccggcatt actttcccca ttggcctgat
    24961 cctgcctagc gaaagggagg aaccgggtca gacaatactt ctgaattgtt gacagatcgt
    25021 gaaatagact ctagggaagg taggggtggg aggaatctgt gtcggaagga gtattcttca
    25081 gcacaatgag ttctactcga cggttagagg cttgtccctg aggtgtatca tttaactcga
    25141 caggacgagt ccccgcatag cctgttgagg acagtcgata atccgcgaga tgatgctggt
    25201 cgatcaaata tcggaccaag gtattggccc tggcggtcga aagatcccaa ttggaggggt
    25261 agagagagga tcgaatgggt tgattgtccg tatgcccttc aacggcaata gaattgggta
    25321 actccttgac gaggccggca atgagatcaa taaattcttt aatctgtgga ttcagcctgg
    25381 cactaccact ttgaaacaac gcatcgtctt tgatcctaat atttaaacca cgttccgtcg
    25441 tggtgatctg caccccttga ggaatattcg ctgcggtaat tgcattggcc acacttaaga
    25501 aggcccgagc attgtcccct gaagaaggtt tgactttcac ctgaatagga gtatttctgt
    25561 ttatgggaag actcacgata gaaagttctc ccacggtctt cttgttgtta aaagaactca
    25621 ccagggattc actgaggacc ttgaatttcc cttcattaac ggaggagact gagtacatca
    25681 ccacaaaaaa ggcaaacagc aaagtaatga aatccgcata cgagaccagc catcgttcgt
    25741 gattctcatg ttcctcatgt ttctttttca cacgctccct ctcaccaaca atccaatggt
    25801 cctcaatttt ctatttcttt ttctccgacg agggtagaaa tccactcaac ttctcctcaa
    25861 tcattctggg attctcacct tgagccaccg ccacaaggcc ttctatcacc ataatttttc
    25921 cgatgatcaa ggtcttcgct ttcaatttga ttttactgcc catggggaga aacaagaggt
    25981 tggccgctcc cacaccatag accgtggcca caaatgctac cgcgatccca ccacccaatg
    26041 ccgaagggtc ggccagattt tccatcacat gaatcaaccc taatacggca ccaataatac
    26101 ccacacacgg cgcatatcct ccaaatgctt catacacctt gcttgtcgtg ccaaattctt
    26161 cttcaaacat ttcgacttct acttccagaa tttcttttat tttgtgaggt tctgtcccat
    26221 cgaccaccaa ctgcacccct ttggccatta atggatcggc caaggacttt atttgccctt
    26281 ccagggccaa gagaccctgt ttccgggaga tatttgataa ctccacgatc ttggcaataa
    26341 tgcccttcat atcaaccttc acatttccaa ataccatgct cgcactggaa agagctttca
    26401 ggaaaattgg aagagggaat tgcagcatca ccgcacctaa ggtgcctccc atcacaatca
    26461 aaaatgccgt cccttggaga atggaactta aatgcccgcc ctccagatgt tgccccccaa
    26521 caatggcgcc aagggaaact atgatgccca ataccgataa aatatccata atggacgact
    26581 ctttcttatt aaaaagtctg attattaaac aagcgttcaa gatccagcag cagcaccaaa
    26641 gacccattca cccgtccgac ccccttatga cattcttcgg ctcctcctcg accagtaggg
    26701 gggggtattt caatacattc ttccgacagg cgtaagactt cttcaacaga atcgacgaca
    26761 aatcctaaaa tcatatgagc gacttctaca accacaattc gagtatcctt ggttggttcc
    26821 gtcccagaca gaccaaagcg ggcccgaagg ttaataatgg ggatcacttt tccacgcaga
    26881 ttcaccaccc cgtccacata atgaggcgca ttcggaacct gagtgatgtc cagcatacga
    26941 ataatttctt gaacccctaa aatatcaatg gcaaagattt cgtcccccaa ctgaaaactc
    27001 accatttgga gaacaactcc acagtcctcc ccaaaatcat catgaggtaa gaccttcgtc
    27061 aaagtattct gcatgacttg tgtccctcca gacgcgtcaa aagtctctct cctgtcccgt
    27121 tccgaccttg tgagaatcgt tgggtaagaa ccatcaccaa gcttttttct ccaatcacct
    27181 caatgacata gctcatacac tgaacctaca tcatggaacc ccatcgcccg tggcatcatt
    27241 ttcctcaata cggacagttc atttttgtta aatgcaatac tcggttcgtc agcaatttcc
    27301 aaccatccac cctcgatttt gcaatttgag ggtaattgtt tctcattgat ccccaacaac
    27361 aggcattggg ctcccttgga ggtatgtgac aatttgaaac aatctcaccg aaacctccac
    27421 aatccccacc aacgtcaatc atcagtgcct tgagacctca acaagggctc tgatagggtg
    27481 aacaccaata ttacttcatt atcatgcatc gaaataaagg attcattcca tggacagact
    27541 agccagttca caagccgacc gattactcct tgatgcaaaa aaagccattc tgcaagaaca
    27601 acatcaacgt tttcaggacc ttcaacttga gggccgctgg gatgatgcct ggaaacaatt
    27661 gcatgtcacc ttggcctgcg cagcggactt actcaaggac tctgtcacca tgctggaaca
    27721 ggttgtcgtt cagaaaaagc ttcctccccc acccgaccaa tcaacaggat tcaacggttc
    27781 catcaaccag aatccccttc cgccccataa ggatgattga caacccactg aacaggggaa
    27841 tctttgcgtt acaccttaaa cgatccgacc aacttctgaa gatccacggc caccacccgt
    27901 aaatcgtgac aggcttgggc cgattgaaag actcctctgg ttgcctgacg tgttgcctgg
    27961 gtcatggatt ctaaatcgct ggcaatttgt cgcgtnnnnn nnnnnnnnnn nnnnnnnnnn
    28021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnttgcc tgacgtgttg cctgggtcat ggattctaaa tcgctggcaa
    28141 tttgtcgcgt ggccgtggac tgctcttccg tagcattggc aatttgttga atcatccccg
    28201 ccgtagcatt gatcatagaa tgaatgtttg ccaaagcctc tccggttttc ttggctaacg
    28261 ccaccccctg tccgacctga cttgtccctt gctccatcga cgaaaccgca cttcgtgtat
    28321 ccttctgaat ttctcggatc atatccccaa tttcttttgt tgccctggtc gtccgttctg
    28381 ctaacttccg cacttcatcg gcaaccacag cgaaccctcg tccttgttca cccgcccgag
    28441 cagcctcaat cgccgcattg agtgccaaaa gatttgtctg atccgcaata tcctcaatca
    28501 cgcgaacgat ttcaccaatt tgatccgaag accgcccgag tgcggtaata atgtttgcgg
    28561 catggaccac tgcgtgagaa acctgctgca tgtccatcac ggattgcttc atctcattct
    28621 gcccgccacg ggctgtttgg gccgtttcct gagcaatccg tgcagcttcg gtagaatgtc
    28681 gcgccacttc ccccgccgtc ctggtcattt ccttcacaac ggaagctgac tgcaaaaaac
    28741 ggccatcctg cccttcagcg gttgaaacta ccccatccac ggtcgcagag agccgaacgg
    28801 tggacgttac aagacggttt gttgcgtgac tgaccaacga caacgccaac tgcatctttt
    28861 caataaatga attgaggtcg tgactgagtt ccacgatttc atcagagcca tttccaaccg
    28921 gcactcgttt cgtcagatcg ccttcgccct gtgcaatatc acgacagata cccattaagg
    28981 catatagagg tttcaacaca agtcgataaa ccaaccaatg caccgcactc acgaaaatgc
    29041 cgacgattcc gatcaaccac attcctgaat ggagcaccga actgaccatc gcctctttcg
    29101 cctgcgttat cggaattgaa agagacaaca ttccaagcac atctcccaat tttttcccgc
    29161 catgacaact tacgcaggcc tccacggtag ccacatcggc actcacccgt cgaaaggtgg
    29221 gaaccccgtt gatcgtctca atttcgctga gaccctttgc cccatttttc agtagatcaa
    29281 gagccttctg ctcgaacgta tctttcggcg cattggcggg attcatcggc tgatcactca
    29341 cataccgggc ctgatcccct cccgccaatc tcaactcctt gccgatttcc tgtatggcag
    29401 tggcgggaaa aggaacggca tcgggatctt gctcatgatc acgagacaca tgcagatgag
    29461 gccccatgga cgattgtttt attttcccca cataattttt ggcaatatag gctctcgtga
    29521 cttcaagttg cgcttgaata atccttcctt cttcctccaa attcacctgc aacctgccaa
    29581 gctcttgctg gtaaatcacg gttaggccgc agacaatcat tccaacggct agggttccca
    29641 gaattaatac gagtttatga ccgattctta agcactgcat acacgtccat caccagggac
    29701 acattgaaca aaggatggta ttatcttttt agcgtaaacc gactaagcga tgtttggaga
    29761 tcctctgcca atgccgcaaa ttgcttcgtt gccgtcgcca ccccctgaat gtggctttca
    29821 ttatcctgac tgagttgcgc gacctgttga atactcgaag agatttttcc ggagaccgca
    29881 gactgttgtt tcgtggattc ggcaaaatgt tgaatcatat ccaccacccg ttgaaccccc
    29941 tggacgatct ccgttaatcg actccccgct tccctggcca gcaccatgcc ggaatgcgcc
    30001 tcttcagttc ccgcctccat cgaaagaacc gcttcgtgag tgccagcctg tacagattcg
    30061 ataaccgtcg caatttcttt tgtcgctttc cccgttcgct cagccagttt tcgcacctca
    30121 tcggcaacca cagcaaaccc tcgaccctgc tcccctgccc ttgcagcttc aatggctgca
    30181 ttgagcgcca aaagattggt ctgttccgca atatcctgga ttactccaat aatgtcccca
    30241 atatccttgg aacgttgtcc taaattctga atacggctgg cggattcctg aatccggttg
    30301 gataccgatt ccatcccatg aatagaagag accacaatat ccccaccccg gacggctgaa
    30361 tcattgaccc ctgaggccgt cgaggccaag gcctgcgtgt gtcgagccat atcatccgcg
    30421 gtagcagaca tttcctcaac cgcttcagca acctgagtgg cttggtgaac ttgtgattgg
    30481 ctggcgcgaa cgacctgatg gcctaccgag gacaattgga ccgacgcttg agacagggag
    30541 tgagcgacgt tccgtgtttg caaaagcatc cggtttaatt catccaacag gttattcaag
    30601 gctaaaccca tttgcccgag ttcgtcttga gtcttcacgg gaacccggtg agtgaaatcc
    30661 cccttcgagg ctttcgccgc cacttcccac acgggtttca gtcggctcac gacttttctt
    30721 cccataaaga ttccaatggg gaccaccacg agaagcccca acagcatctc caacaaggtt
    30781 ttattggtca ggctcctgac atgactgaat gcttcattct ccggaacttg cactaacacg
    30841 gaccagtgca tccccaacga tccccccgcc cctgacatcg ggctatatcc gatgacttga
    30901 ttcgcgtttt tcccgggatg aaaatttctg gcgtttccgg attggccctg aatcgccaat
    30961 tgtgcggcct caaggcccaa ctgaggcagg ttttccttaa acaccacatt ttctaaatcg
    31021 tgaatcattt cctctgttcc atggacagcc ggagcatatt ccatcagcgt catacctttg
    31081 ccatcctgta gggttagttc cgcatgggga aacccggcct tttttaaatc caggtatgcc
    31141 tcttggaaaa aggcttcaat ttttgaaaac ttcatgcgat gactcccata cccaaccact
    31201 cgccccttag catagagagg aaccgagagc ccgattgtca aactgctctc ttgggggaag
    31261 agtgcctgca catcctgatc gaccatgacg ttttccacaa agacctcttg gggcacattt
    31321 cttgtcaaag aagactctgt caagcgtata ctttcttccc cctgttggag ggctcgaaac
    31381 cacggagtca atctataact ttttccgtac aacttttccg tccgaagagg caggccttct
    31441 gcatcttgat catttacggc gatcagattg ccatccaaat ccaccacttg aaccaaagaa
    31501 tacatgccgg ttgcctgaac atacgcattc aatacctgga cgaattcctg tgagggacgg
    31561 acagcctgtt cccataattc tgcaccaccc aaaattggac tccgactcaa gatctgggca
    31621 tccgcagaac gctcagcgag atagagggaa attttttcaa caatcacttt tgccacgtct
    31681 tcgtattgaa caccaacctc ttttttcaag acctctccag tttggaacaa cgaatacact
    31741 tgaacactca aaggaatcag cgaaaaaaac ataagaatcc agactaattt tgtcgtaatg
    31801 cctacattcc acgtgggctt cctcattggt atcctcttgt taatcttttc atggatctcg
    31861 agatggggta gtacattctt ctcaacaatt acaataatta ttctttcctt tacgaggaca
    31921 ccacgactcc cgaagaattt ccgagatgtt gaactaattt gtcacgaaga tcggttggat
    31981 gaaatttggt cacataatct gtcacaccca aagcctttcc tttatccaca ttacactgtc
    32041 cggacaagga ggaatgcatc aagatgggaa ggccctgcaa tcgggaatct tcacggatag
    32101 cctttgtcag ggaaaatccg tccatttctg gcatttcaat gtcggtaata accccaacga
    32161 caaagtcgaa aatggttcga tccgttttgt cacccaactc cgcatactgt cgtaaccggg
    32221 tgagcgcctc ttttcccgtc tgtgtttctt cgtatttcat gcccaaccgt tccagcgttt
    32281 tggtaatttg ttttctggcc acaagcgaat cgtcggcaac caacacacgt tttccggcta
    32341 attcatcgtt cagttcaatc ccaacgagga cttcctcttc acttctgggg tgcaattcgg
    32401 acaacacctt ttccacatct agaattaaca ccattcggcc atcctctaat ttcgtaatag
    32461 ccgtgaccgc cccttgccgg ctggactgga ccatcggggg gggaatttga atccgttccc
    32521 acgacatacg aatgatccga tcaacgccgc ctaccaaaaa tccctgtttg ttatcgttat
    32581 attccgcaat gatcaacttc gcgccaaccg ggtccaaggg cccgagtcca atcgcctgtt
    32641 ttaaatccac taatgcgata ttttctccac ggagattcac taaacccagg attcggggat
    32701 cggagtccgg aattttcacc acgggtggga gcttcatgac ttcgcgaatc ttaaaaacat
    32761 taatcccgta agtctcatta gtcccaaaat ggaacattaa aagttccatc ttattggccc
    32821 cggccaaccc ggtccgttga tcaatttctg ccatccattt tgccattacg ctttcctcat
    32881 ccttccactt accaaacgga gcacaggtca gccttcttta tagttccacc gctgtcctca
    32941 tgcctgtcgg gataaagacc caggcagtct agtcagccct actcactttc cctaggcctc
    33001 acgcttcact gttttaaaca ttagcgcata cccgtatacg ttgaagtctg cacttcccgt
    33061 actaaatcgg ccatatccaa aatcaggacc actttgccat ctccggtaat ggtcgccccc
    33121 gccacgcact tcacgttggc caaaaattcc cccaaagact taatcacgac ttcctcttgt
    33181 gcccgcaaac gattcaccac aactccaaac tgttgttcac caatcttcac aactacgaca
    33241 tagcattcaa caccagtccg gtcccgggga atatgaaagg tttccccaag gtccaggaga
    33301 ggaagaatgc gttctcggag attcaatacc gcctgtccat tgatactttt aatttcatcc
    33361 ttggtgactt tgacggcctc aactacggaa gccagcggga tggcaaatgt ggcacattcc
    33421 acctcgacca ttagtgcttg aataatggcc acggtcagtg gaagcttgat cgtcacgcga
    33481 ctcccctgac cgggatcaga atctaactcc aaactcccat tcatgcgact gatattggta
    33541 cgaaccacat ccatacccac cccacgaccg gacacatcac tcacctggtc cgccgtacta
    33601 aatcctggaa gaaatattag attcatgatt tcccgagggc tcatgacttc caattccgct
    33661 tgagccacca agcctttttc gagagctttt tcagcgattt tctcaacttg taacccccga
    33721 ccattatctt ccacccgaat aacaatactg ttcccttcct ggtgggctga gagtcgcaca
    33781 tagccttcgg aacctttacc gtgttgctta cggtccgcag cagtttccaa gccatgatcc
    33841 atcgaattcc tcaccaaatg gaccaacgga tcacttaatt cgtccgccac ggacttgtcg
    33901 acttcggtat cctcccccag taattccaaa cgcacttgct tacctaactt accggccaaa
    33961 tcccgaacca atcttgggaa tcgggaaaac accttgcgaa taggaaccat ccgtgttttc
    34021 ataaccgcca attgcaaatc ggaggtcacc agattgagct gcgccaacgt cacatttaat
    34081 tcccggatgg caggactggt ctcctctttc tcctccagcc cattccccag tttaataaga
    34141 cgattacgat tcaaaaccag ttcccccacc agattcataa catgatccaa gcgtttggtt
    34201 tcgactcgaa cggtctggtc ctcttcttta ccccctgatg ccttgccggc cggagcattc
    34261 gcggcttgaa cggttcgagt cgtggcttct ttcattttat ttaatacatc agataacccg
    34321 actgggcagt cagaagctcc tgctgattcc gccacatcct caatcccggc gggtacatca
    34381 tgctgcgggg cttcacgcaa agaagaccag gaagaggttt caggaggcac taccacagga
    34441 gaaagcatcg tttctgccgt aggtggagaa ggggctgagg tgactgtgtc cttctcggcc
    34501 ttatggggag gtgagacgga cagcatcaga tcctcggcgc tatccatggt taacgccaat
    34561 ttattcacaa tgccaaaggt ctcgacatgg gaatcttctc cggtctcccg aatatcagac
    34621 tggagcattt tcaccgcgtc caccgcttcc aaaataatgt cgataatgaa cggagaaacc
    34681 gacatttccc cctgtctcag tttgttcaac aaactctcgg cttgatgcgc cacttcaacc
    34741 aaatgggtaa accccaaaaa tccggccgat cctttcatac tgtgcataca ccggaaaatt
    34801 tcgttaagca gttcgctgtt ggaaggctcc gtttcgagtt ttacgaaatg ttggtcaagg
    34861 gcttccagca tttccgagct ttccgctaaa aagtcgttga gtatttcttt catttcgtca
    34921 ttcatcgatc acctgttctc agctcctaga cttttctctt tcatcccatc ccccaccctg
    34981 agacatcggg tttgaaggtt cgtgtatagg gaattaggac attccgtatt cagacatgat
    35041 gttatcgacc aggtcttgtt gaagggccgt agtttttgag gattccaact gacgcagcat
    35101 ttcatacccc cgtccctcac ccgatgctcc ggtctcaccc ttttgttgtt gaattccaaa
    35161 aattaccacc aactttaata atttgtgttg aatttcattt aaaatcgcca cgagctttgc
    35221 cacgcgttgg gctacgagat cctggaaaga caacgctaca tggatattta acaatctacc
    35281 ttcatcagcc ttcaccataa ccaaaattgc atcaagatcc ttgacggccc tcccggccac
    35341 attggcacgc aattgttcca aatgatgttg gatcccctcc cggttccgtt ccaattcctc
    35401 tgtcaggctg agaaccttat gcgttccgtc ctccgtcatt tttgcaagat catacaaatg
    35461 tgatgtggct tgaggcagtt gatctgaggt ggaggtcaca ggagattcca tttcccgaat
    35521 ggttttggta atgacttcca cgtacttggc caattcctcc atttctgcat taatggaacg
    35581 tttttcattc aaaacctcct cgggcatctc tccatcgcct ggcacctcgg tctcaatcat
    35641 ccgatccacg agcataagaa tctcctttat atcgtcatat tcggaagatg ttctgccccg
    35701 ctatgacgtt ttggccggct gaacatttgc aaatattttt tctaattttc cttgcaagac
    35761 ttccgcggta aatggcttaa cgacgtagtt actgaccccg gcttggactg cttcgattaa
    35821 attttccttt tgcgcctcag cagtcaccat taaaaacggg agcgttttta attctgcatc
    35881 gcttcgaacc gcccgcaaaa gctcaatacc ttgcatcacc ggcatattcc aatccgacac
    35941 caccaaacca aaaccaccgc ccttcaattt cgtcaaagca tcctgcccgt tttcggcttc
    36001 catgatgtcc gaaaagccga tttgacgtaa cacatttttg acaatccgac gcatggtaga
    36061 catatcgtcc accaccaaca ctttcatccc ggtatcaatc atgatgcgct ccttacgcct
    36121 tggtccataa aaccccaggc aatgtgggta ttcccattaa agaatcatct tgaattcgtt
    36181 gatattctta ttatcctgta ctaacctggc ctattcgaaa ttcggttttc ttctcttgtt
    36241 tctttagccg tccttataaa atttcacatc tcggctttcg ttctttcatc agaccatctt
    36301 ggtagattgc ccatgccgta gactatcgat aatgtatttt taagtgttcg ggaccggaac
    36361 cttgattgaa gtcaatttcc cctggtctta aaaccttaaa caatattcga ggactcagga
    36421 ttgaattgct ttggacagac aaggttagga gggaagagga agttttatct cgtgcgacgg
    36481 aaaagccatg aacgataacg cctctgacca cgttcaaaga tcagggatac actcacttgc
    36541 tcattccggg gcatcttgcc ctcagaatat gagcaaggaa ttcatgtggc gtctggccta
    36601 tcctccagga agaaagtcac gggccacctg cggaagttaa ttcaaactaa acctgtcccg
    36661 agccgccggt tggggcccag caccaaccat cacggactct cctccagcat tttgtaccca
    36721 ggcttcaatt tcctcccgaa caaaacgcca gtgtttccca actttatgcc ctgggagtcg
    36781 acgttctctc gctagcttgt agattgtcga ttttgggact ttcaagaacg tcgatacttc
    36841 acttaaggtc agcaaatctt tcattgtggc accctcatta gagtttggtc agtaatgtgt
    36901 gtgatatcgg actaatcatg tgaaaacttg agacttcttc ttagatagta ggaaattgtg
    36961 ctctcttcac ctaccgatcc cggcttacga aacatggtca ttccgtgttc cgccctcagc
    37021 gaaagagata gtttgggaag accgtccctc cttttctcct tgaacaaaaa tcaaacgttc
    37081 aaatacacaa attagctgtt taaccctctg ctctaattgg atgaccgctg aatgaagaag
    37141 cgaaaatgtt gccgagacag ccctgcattc aagttgttca gcttctttcc attctgacat
    37201 ggcatgtacc gcacccagcg cccctttcat ttgatgagcc agtctttgaa gctctgagcg
    37261 attttgcatg tgaatagctt gatgcaaccc tttgatcaaa tccggtgttg tgaccttaaa
    37321 tatgtgaaac aaggaataca gcaacccctc atctccctcc agggcgtgca aagcggctga
    37381 gacgtcatag agcgttctgt cctcagggaa ctcggaggga tttcctgcat ggtgttcccg
    37441 atctaagtct ctagtggcaa ccgaaaaagt tcctttccga tcagtcttgt tatctgtctt
    37501 cgatgaggac ggatctatga cacctccagg aacaaaccct acccatttcc tgagtattcg
    37561 ttcaaagtct cgtaaatgaa ccggcttaaa aagacaatca tccatgccat tctccaggca
    37621 ttgttgtcca tgctcagaaa gcacatgggc tgtcatcccg acaatgggaa tatggtgaac
    37681 cggaggagac gccgacccat aatggcgatg ccaataggcc gatgtatgga gaaatgctcc
    37741 agcatgttcc aattcccgaa tatgctgagt gatggccagt ccatccatgt cgggaagttc
    37801 ccagtccatc agaatgcaat ccacatctgt ggattgcaac aatgtcaaag cttgggaccc
    37861 cgtgtccacg atcgtcactt gacatccgag tttccctaac atccccgctg cgacccgttg
    37921 attgacggga ttatcctcca caaccaacac cgaaggtcct gcttgtttct ggaggagggg
    37981 ttgctctaac acctcaagcg tcccctgcac tttctcaacg gaaagccgct cagggggtcc
    38041 tggtatatct gacggtccag gatcgtccat ttcgccaaat atgcattgaa aaaattgctt
    38101 ccggtgtaag ggaagcgtga tcatcccatc aaaccccaag cttttttctt catcatcctt
    38161 gccataccaa aacggcttaa gacccaaaac tttgacatcg tgaaacagcg aaaaagaacg
    38221 cattcgtcca acccattccc gaacagccgc tttctccaga cgattactga gaataacccc
    38281 tgcgacgtga cgttctgatt cgtggacact ttcgaacagg atgttcgcct gttgaaggct
    38341 ctctatctgc acaaccttga cacgcacttc ctgaagaagt tgtcccatca cccacccaga
    38401 cgattgttga ggaccgatca cccagacctc tttgccagca acggatctga cgtcagggcg
    38461 tggcgcatgt atagtccgtg atgcccgaca gggaatatca caccaaaatg tcgatccctg
    38521 atcgggttga ctcctcaccc cgacagtccc gtgcatcaac tcagctaatt gtttgcaaat
    38581 cgctaaccct aaaccggacc ccccgaattt tcggctcatg gagctatccg cctgggtaaa
    38641 cacgtgaaaa attttttcct gaacctcctc ggacacacca aggccggtgt cttgggtcga
    38701 caatcgaacc cacaactgag tctcttcgcc tgactcaccg gaacgatcat tcagcccatg
    38761 cagggaacca ttacacgcag gattcgctgg atgaatcagc gaaccgcgca gcaaaactga
    38821 tccctccatc gtaaatttca gcgcattgga aagataatta catagaattt gtcgcaaacg
    38881 gtgaggatcg ccaaacagtt cgaagggcac atccggatcg acataggctg ccaaatccag
    38941 accctttcga tgggcccgct ctgaaaacag atcgacgacc tcttcaaata gagacgtcaa
    39001 atcgaatcca atgctttcca ataccaattg accggattca agtttggaaa aatccaaaat
    39061 atcattaatg atggtaagca acccctcacc cgacatacgg ataatgttga cacattcctg
    39121 ttgctcttta tcgagagatg acgcaagcaa gacctccgcc ataccaatca ctccgtttaa
    39181 gggcgtgcga atttcatgac tcatcacagc caggaatgcg gccttggctc gagtagcaga
    39241 ttcagcctta attaacgcat gatccagttc atgatttttt gcttccaagg tatccgcaaa
    39301 ggccgacatt tctgctgcgg ctcgctttgg ctcggtgata tccatgccat atccgcgaag
    39361 taatcccaaa tccgcatggg gagcaaatgt ccaggaatat tgcctctccc caacactcac
    39421 ttcgtaatgc atctctagat gtttttgtga caaacaacgt ctagccaaat ccaaaaaccg
    39481 atccggcaaa gccgtggaaa aaccgtcctg accgatatgg gcttcttcca tcaggcgaac
    39541 catagcggga ttggcataca gaagttgccc cgccgcatcg acttcaatca cggggaaggg
    39601 attttccttt ggaatggacg ctaatcggcg tgtttcttcg gtcaatttca tttctgaact
    39661 gagatctcga aacatcaaca ggtatcccga agcctccacg tcatctacca caagcccttt
    39721 taattccacc caacaccatg tcccctgccg acttcgcaaa cgagtccgtg gaacccatag
    39781 catggttttt tccagtagta atcctgaaag agggcagatt tctgtatgat ttttctcaca
    39841 ggggaccaga cattccaaaa gatcatgaca cgaaacattc gtgggacagg cggctcccca
    39901 ccctaacaga cgtgagccgg ccaaattcat atgtgtcaat agaccattcc gatcaactag
    39961 acacaccccg atcatcaggc catcccacag agtctgaaac aaaagagaat ttgcccacat
    40021 aagttatgac aggatttctc gaaggtttat agaagaatcc gcagaatcaa gtactttcgt
    40081 aatcttcact tcatacttta attctgaagg gatccctaaa tttcttactt ctggcttctt
    40141 aaggatttcg ccagtgcaag tcttgataat tttaacctgc ggtctcacac tcatctcagg
    40201 attctgtttc accaccacgg catcctcacc agtggaaagt tgaacaaccg tgcccaccgg
    40261 atagaccccc aacacccgaa tcatggcttc gacaaaccgt gtcgggaact tcccactttt
    40321 ggcttcttgg tagactcggc gaattgctaa agccacagga aatgatccgg aggaattccc
    40381 aacagcaagc aactcatcaa cataatcagt gaggcccaat aaagtcccag aatcagagac
    40441 cgggttcccc cccataacag tggaatatcc agatccgtct agataagcat gatgctcctg
    40501 tacaatttga acgatttcct gagaaaattg cgatttctta ttaatcaggg ccacactcat
    40561 atcgacatgt tgttgcaaca acgaataatc aatccccatg cttccccacc gtgcctggac
    40621 gacgttgagc ggaagccgca tatagccgac atcatgtaaa agcgcggcaa cggcgacctc
    40681 ttcaagctga ttgacctccc acctaagttg aaggccaacc agaatcgaca acaaggcggt
    40741 atcgacaaca tgcgagtaaa gagtgggatc gaactgttca agatgctcaa tcaggaggat
    40801 ctcggcgacc acagtagggt gagctaatgc cttttcgacc aatgccttgg ccgcctcctg
    40861 catagcgata tgaggaatga tctggccggt tttgacgcct tcgaacatgt cttccaaggc
    40921 tgcaatcgta tctccgcgca accggattaa ccgttcttga tgttcatcct cccttccatc
    40981 ctttccttct ggagcgtatg acctgggcgc ttggctgtat ggatcagcct gactggaaga
    41041 cgtttctagt tccggatgac actcctgggt gtcctcgact ggaaaggatt ctgcatcggt
    41101 cgacacatcg agacccttgg cagtatcaat ttcgacttct tgaattcccg actgacgcaa
    41161 ttgcttcaca tcgtgggagg atagaagtcg gcgatgacga aaaaacggcg acttccacca
    41221 ggaatcgagg attcgaacca aatacatgcc tgcacatatc tgtgaacagg caagacgctt
    41281 tcgagtcggc atagatgagg gctctccgta agaaaggcct ttttttatta ttgaacgggg
    41341 aaagtcagga gcagtcctgt tctctcccac cttcgccctc catcatacac tggcattccc
    41401 gacatttttc atcggtaata ctcttttgct ttttcagata gatccccact aacgaccggc
    41461 ggagatgatg gggaaatgta gtcattcctt cctttcccct cacactcatc ttttacgttc
    41521 caccatccaa atctgggttc cattggtgag agagaacatc aagggcggaa aaagatgaat
    41581 atggattaca ctcctgatgg cacgaccggc aattgaggta cctcatcaat tgtctgatat
    41641 atgggaatca ttttggcgac attggctaaa tccaagacct gacgaacata cgtttgcggg
    41701 ttcaccaggc taaaaggaat ttttctactt ttgagtgttt gatgggcaat caccaaaaga
    41761 cccaaggcgg aactatccac aaaagagacc tgcccaaaat ccaagactat cggcgatccc
    41821 tctttcattg cctcatctaa gctgtctttg aacgtttttt ttgcaccaaa gtcgaaccgc
    41881 ccagccaaat gcatgaccac ggctttctca attttttctt gcgtgacatt cataaactcc
    41941 tccatggtgt atgacaagaa tccttcttga caatcctgtg atcatccaat tcaccttatc
    42001 ggtggaagtt tacaaagaat taaccattcc cgctctatcc tttaaacctt agattgcttt
    42061 ttttaacaac taagagacta gagatgatgc gaccaagttg gcaccatacc gtcctatgca
    42121 cccggacttt ctgacaattc gaatcgagac cagcacgcag tttgtcctta tgtagtgagc
    42181 aatccattcg agggaacctg acgaacattc tcctgagccc accgaagaac atagacaaaa
    42241 tctattgaaa tattaggttc ttcgagtgag gcagtttgaa aagattagcc aagacaaaga
    42301 actaagccct ccgttaatcc gtccttactg ggtacactga ggaactgttt accgacacac
    42361 gcttgaaatg atcagccact gtggatgtcg ttttcgtggc aaacaaatga aatacccatt
    42421 agacgcgaga atgcctcaaa tacgccgaag aatgtctttt ttggtcttca aatcgaaatt
    42481 cgcgataaca gttcggataa acaccagatc catctttaaa aggaccagcc atgaaccagt
    42541 aagattcatc tccggaaatt cgatcatatt ccgcacatct tcacgaatct gccattccat
    42601 ccactgccat gaaaagtagg tattgaggaa atggagggaa agacctcgac ttacatcgaa
    42661 tgatccatcc tgtccacgtc tgatcatttc ccgggaaata tcgatggcga acagacgaat
    42721 atttcaggcc atcatttctg aaaaatgatg caaaatcatt aagaaaatta aacaggtttc
    42781 ctctctgctg gaactgaccc cgcatcagat agataaacgc ttggcgtccc aattggcttg
    42841 aatcacttcc ggcaggattc gttctttttg cggagacgga tctaacaaag gtcctgagga
    42901 ttttttgtag tcttgtttca attgaagaaa aatcgaataa taaccatcgg aaagacaatc
    42961 atgttcatgc ctcagtatcc gacataccca tgaggcccca ttaacgggga gccaacattt
    43021 tcaaatgagc caacattgcc gaaggaatat cgtttaacgg aagaattttg tccactcctc
    43081 cagccttgat ggcttccttg ggcatcccaa aaaccacaca cgatgcttca tcctgggcaa
    43141 agttatatgc tccggcctgt ttcatttcga acattcccac agctccgtcg ctccccatgc
    43201 cggtcagaat aaccccgaga ctattccctc cggcataccg ggcgaccgat cgaaacatga
    43261 cgtccacgga aggacgaaat cggttaacct gtggctcttg attaagtgag acataatagc
    43321 gggccccgct ccggcgaagt tccatatgat aattacccgg cgccaacaag gcctgtccgg
    43381 gcatgacgct gtctccttcc cgagcttctt tcacgtgaat ctgacacagg tcatttaacc
    43441 gattggcaaa cgatttggta aattgttccg gcatatgctg cgtcataata atgggtgggg
    43501 tattcggtgg aagcacctcc aataattccc ggagcgcttc ggtcccaccc gtagacgccc
    43561 cgatagcgat aattgtgtca gtagtcttaa tcattgcgga ttgggcagcc agcagtttaa
    43621 tgcgttcagt acagtcggcc ggtggggttc ggcttttcac gctggcctgg gccgcgatct
    43681 tgactttgct tataatttga tgagctaagt ccgacagacc atgctgaata tccaccgtgg
    43741 gcttcgtcac aaaatccaca gcaccggctt ccaaagcctg taacgtggtc gccgcgcctt
    43801 gttcagtcaa ggaactcacc atcaccacag gcatagggcg cgccgccatg attttctgca
    43861 aaaaggtgag tccatccatt ttaggcattt ccacatc
//
LOCUS       DAORAQ010000006        43796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_195_length_43796_cov_68.280926, whole genome shotgun sequence.
ACCESSION   DAORAQ010000006 DAORAQ010000000
VERSION     DAORAQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 43796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43796
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195_length_43796_cov_68.280926"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="tuf"
                     /locus_tag="PKM72_01185"
     CDS             <1..233
                     /gene="tuf"
                     /locus_tag="PKM72_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNP59417.1"
                     /translation="RHTPFFNGYRPQFYFRTTDVTGVVQLNPGVEMVMPGDNVSFEGE
                     LISPIAMEQGVRFAVREGGRTVGAGVVTEIVA"
     gene            269..580
                     /gene="rpsJ"
                     /locus_tag="PKM72_01190"
     CDS             269..580
                     /gene="rpsJ"
                     /locus_tag="PKM72_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNP59418.1"
                     /translation="MDRDRRIRIRLKGFDYRVLDQSLREIVDTVRRSGARIAGPVPLP
                     TRIEKWTVQRSTHVDKKSREQFEIRTHKRLLDIMEPTPETMDALMKLNLAAGVDVEIK
                     L"
     gene            640..1257
                     /gene="rplC"
                     /locus_tag="PKM72_01195"
     CDS             640..1257
                     /gene="rplC"
                     /locus_tag="PKM72_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNP59419.1"
                     /translation="MVKGLIGKKLGMIQVFDEERRLIPVTVIEAGPCGIVQLKKKSTD
                     GYEAVQIGFDEVPERKQSKAQLGHLKKAGNKVWRHLREFDSAGDGEVGSMVDVNLFTE
                     GEPINVQGISKGKGFQGVIKRHNYAGGPASHGSMFHRAPGSIGSSSFPSRVLKNKKLP
                     GHMGNKQITVRGLKVFGIKSEENILLITGSVPGPVGGVVIVRKVG"
     gene            1257..1886
                     /gene="rplD"
                     /locus_tag="PKM72_01200"
     CDS             1257..1886
                     /gene="rplD"
                     /locus_tag="PKM72_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNP59420.1"
                     /translation="MQSIDAFPVIGQDARPVDKVDLTDGVFSSTVNPSLVHRAVVMQQ
                     SSKRQGTASTIGRGEVRGGGKKPWKQKHTGRARSGSSRSPIWRGGGTVFGPQPRSYAS
                     TLPKKMYRAALKGALASKVGDGLIVLDDLALPEMKTRELVKLLLNIGASGKVLLVIHD
                     GLADFFRLGKNVKNLKILHGKDLNVYDVLWCEKMVVTRVELERIQEIWV"
     gene            1888..2175
                     /locus_tag="PKM72_01205"
     CDS             1888..2175
                     /locus_tag="PKM72_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNP59421.1"
                     /translation="MNPHDVLIRPLLTEKITAIREIKNAIAFSVHRQATRIDVRRAVE
                     KVFSVKVASVNMMNVKGKKKRQGRFTGKRSDWRKAFITLKEGEKIELYESA"
     gene            2219..3058
                     /gene="rplB"
                     /locus_tag="PKM72_01210"
     CDS             2219..3058
                     /gene="rplB"
                     /locus_tag="PKM72_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNP59422.1"
                     /translation="MPIKEYNPTSPGRRGMSAVTGEGLSRKRPLKSLTTSNSKSGGRN
                     NAGRITSRFKGGGHKRLYRKIDFKRDKFKVVGKVAGLEYDPNRSARIALLYYADGEKR
                     YILAPFGLKVGDSVQSGSGVDVRVGNAMPLMEMPLGIVVHNVELKPGKGAQLARSAGA
                     SAQVMGRDEGYVQIRLTSGEMRKILGTCMATIGQVGNTDHNNVTIGKAGRSRWLGRKP
                     HQRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGQPTKGFKTRKPRNQSSRFIIAGRKK
                     KSR"
     gene            3074..3361
                     /gene="rpsS"
                     /locus_tag="PKM72_01215"
     CDS             3074..3361
                     /gene="rpsS"
                     /locus_tag="PKM72_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNP59423.1"
                     /translation="MPRSVHKGPFVDDHIAKKVEKAREGRESKVIKTWSRRSTIIPDM
                     IGLTFAVHNGKKFIPVFITDNMVGHKLGEFAPTRFFKGHGHARSEKAVALK"
     gene            3415..3753
                     /gene="rplV"
                     /locus_tag="PKM72_01220"
     CDS             3415..3753
                     /gene="rplV"
                     /locus_tag="PKM72_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNP59424.1"
                     /translation="MTEAKAILRHVRLAPRKARLMVDLVRGRNAAEALAILKYTPRSA
                     ARVVEQLLFSAVSNAGQKDLGDPENLKISRAYVDGAQIYKRFRPRSMGRANPIHKRTS
                     HITIVVCPAN"
     gene            3803..4468
                     /gene="rpsC"
                     /locus_tag="PKM72_01225"
     CDS             3803..4468
                     /gene="rpsC"
                     /locus_tag="PKM72_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNP59425.1"
                     /translation="MGQKTHPYGFRLGYTRTWHSRWYAKKDYAKLLHQDLSIRDVVKK
                     RLYHAGISSVEIERSGNQLKLIIHTARPGIIIGRKGAEVDKLKATLEKEYGNEVFVNV
                     KEIKKPELDAQLVAENIATQLEKRVSFRRALKRSVASSLRLGALGIKVYCAGRLGGHE
                     IARTEWYREGRVPLHTLRADVEYGFAEAKTAMGQIGVKAWIYKGDAQIPSLEKSEPSL
                     GFL"
     gene            4506..4928
                     /gene="rplP"
                     /locus_tag="PKM72_01230"
     CDS             4506..4928
                     /gene="rplP"
                     /locus_tag="PKM72_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNP59426.1"
                     /translation="MLAPKRVKFRKVQKGRMRGKAYRGGEIAYGVFGLKAMEPGRITA
                     RQIEAARIAMTRHVKRGGRIWTRIFPDKPITKKPAEVRMGKGKGNPEYWVAPIKSGRI
                     LFEMDDVSQIVAEEALRLAGHKLPVATKFVIRGEIPVL"
     gene            4938..5126
                     /gene="rpmC"
                     /locus_tag="PKM72_01235"
     CDS             4938..5126
                     /gene="rpmC"
                     /locus_tag="PKM72_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNP59427.1"
                     /translation="MEMDEIKNLEKIELLEKVNKLKQELFHFRSQLALGRMENPMRIR
                     ETRKNIARVQTVLRQKAT"
     gene            5178..5426
                     /gene="rpsQ"
                     /locus_tag="PKM72_01240"
     CDS             5178..5426
                     /gene="rpsQ"
                     /locus_tag="PKM72_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNP59428.1"
                     /translation="MPRSLTGKVVSNKMQKTVVVEVVRIERHSLYGKVMRKKTKIKAH
                     DEINQCLIGDQVNIVYARPLSKSKHWRVAEVLTKRVTT"
     gene            5486..5854
                     /gene="rplN"
                     /locus_tag="PKM72_01245"
     CDS             5486..5854
                     /gene="rplN"
                     /locus_tag="PKM72_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNP59429.1"
                     /translation="MIQNYTYLDVADNSGAKNVMCFHVLGGTKRRYGSLGDIIVVAVK
                     EAIPKASVKKGDVMKAVIVRTKKGRRREDGSHIKFDRNACVLVNAQGDPVGTRIFGPV
                     ARELRKKKYMKIISLAPEVI"
     gene            5865..6197
                     /gene="rplX"
                     /locus_tag="PKM72_01250"
     CDS             5865..6197
                     /gene="rplX"
                     /locus_tag="PKM72_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNP59430.1"
                     /translation="MSEKFRIKKGDMVMVIAGKERGKTGKVLQVQTKDARVTVEKLNI
                     LKRHTKPNAKNKQGGILEREGFIAISNVMAFCESAKKPSRIKMKTFDDGRKVRVYQKD
                     SNEVLEKS"
     gene            6290..6823
                     /gene="rplE"
                     /locus_tag="PKM72_01255"
     CDS             6290..6823
                     /gene="rplE"
                     /locus_tag="PKM72_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNP59431.1"
                     /translation="MKAMYQEKVIPAMMKEFGYQNPMQVPRVERIVLNIGMGEAVQNV
                     KLLESAAAELEQISGQKPVLTRSKKAIAGFKLREGIPIGTKVTLRGARMHEFLDRLVS
                     VALPRIRDFRGISPKAFDGRGNYTLGLKEQLAFPEIKYDDVASIHGMDITFVTTAQRN
                     DEAKSLLTHLGMPFRKP"
     gene            6842..7027
                     /locus_tag="PKM72_01260"
     CDS             6842..7027
                     /locus_tag="PKM72_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNP59432.1"
                     /translation="MSRLALKNKAKRKPKFTVRHYNRCALCGRVRGFLRRFLMCRICF
                     RFLSLKGDIPGVTKSSW"
     gene            7091..7498
                     /gene="rpsH"
                     /locus_tag="PKM72_01265"
     CDS             7091..7498
                     /gene="rpsH"
                     /locus_tag="PKM72_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNP59433.1"
                     /translation="MSMTDPLADLFVRIQNAGRRGHDLVKIPSSRVKKDILRIFKEEG
                     FIRDYKAATGANGHPVIEVSLRYAPGRQKKSFITGIKRISKPGCRVYAGKEDLPRVMR
                     GLGLAILTTDRGLLTDEQCRSSQVGGEILCHIW"
     gene            7544..8083
                     /gene="rplF"
                     /locus_tag="PKM72_01270"
     CDS             7544..8083
                     /gene="rplF"
                     /locus_tag="PKM72_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNP59434.1"
                     /translation="MSRIGRKPISIPKGVEVRVLDGNVLVKGPLGELQWNLNPGIQIS
                     IENEEVSLTRNSDSAKLKALHGLTRAELANQVKGVKEGFQENLEVMGVGYRAQIQGKE
                     LSFSVGYAHPVSMGLPKGVEASVDKQTSISLKGIDKRMVSLVAAQIRGMKVPDVYKQK
                     GIKYVGEILRKKAGKAGKK"
     gene            8093..8458
                     /gene="rplR"
                     /locus_tag="PKM72_01275"
     CDS             8093..8458
                     /gene="rplR"
                     /locus_tag="PKM72_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNP59435.1"
                     /translation="MNILEKQKRLSKRSRRVRLRIVGLSSRPRLSVFRSNSHIYAQII
                     DDSAGRTLVSASSCDPGLKDKIKSGGNIEAAKMVGQAIAERAKAIPIELVVFDRGGRL
                     YHGRVKALADAARSGGLKF"
     gene            8503..8997
                     /gene="rpsE"
                     /locus_tag="PKM72_01280"
     CDS             8503..8997
                     /gene="rpsE"
                     /locus_tag="PKM72_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNP59436.1"
                     /translation="MRVNVEELNLKDKPVVINRVAKVVKGGKRFSFSALVVVGDGDGW
                     VGVGKGKATEVPSAIAKAVAHAKKNLIRIPLKSNTIPHEVHGYFGGEHIVLKPAKQGT
                     GIIAGGAVRSLLEVAGAQNIVAKTLGRGNPFNAVRATIQGLSQLRDPHEVRELRRTAV
                     GMED"
     gene            9035..9223
                     /gene="rpmD"
                     /locus_tag="PKM72_01285"
     CDS             9035..9223
                     /gene="rpmD"
                     /locus_tag="PKM72_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNP59437.1"
                     /translation="MVTPTNLSITLKRSTIGHPDKMRVVLLGLGLRKIGQTVSRPDTA
                     QVRGLIYKVRHLIEVAGS"
     gene            9220..9669
                     /gene="rplO"
                     /locus_tag="PKM72_01290"
     CDS             9220..9669
                     /gene="rplO"
                     /locus_tag="PKM72_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNP59438.1"
                     /translation="MKLHDLHPSPGSRTKRKRLGRGPGSGLGKTSGKGHKGLLARSGR
                     ANQAGFEGGQMPLARRLPKRGFTNIFRIQYSVINLKDLVAQEKTTNFNPTVFNEIGLT
                     KGRNQQIKILGSGEVDRPIVVEAHKFSDSAKQKIENAGGQVKVIGRA"
     gene            9662..10975
                     /gene="secY"
                     /locus_tag="PKM72_01295"
     CDS             9662..10975
                     /gene="secY"
                     /locus_tag="PKM72_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNP59439.1"
                     /translation="MLERLVTSIQNIFKIPELRSRVIFTLSMLAVYRIGAHVPTPGIN
                     NEELFKFLTERGGALMGFLDIFSGGALSRLTIFALGIMPYISASIILQLLTVVVPHLS
                     KLAKEGERGRKTIIQYTRYGTILIALIQGFGIALGLEGMNDGAFVLDPGWPFRLMTVI
                     TLVAGTAFLMWLGEQITERGVGNGISLIIFSGIVASLPSAVVQTFDLYQVGQISLLLL
                     LILGVVMLAVMGAIVFLESGRRKIAVQYAKRIVGRRVYGGQNTHIPLKINTAGVIPPI
                     FASSIIAFPATIASFIQVPWVQSIGAQLAPGSLLYTMLYVGMIIFFCFFYTAVVLNPV
                     DMADNMKKYGGFIPGVRPGQKTADFIYKVLTRITFAGAIYLAIVCVIPELLIYRLNMP
                     FYFGGTSLLIVIGVGLDTAQQIENHMLMRNYEGFLGKGSLKGRSG"
     gene            10980..11630
                     /locus_tag="PKM72_01300"
     CDS             10980..11630
                     /locus_tag="PKM72_01300"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNP59440.1"
                     /translation="MRVIFLGPPGVGKGTQAEFIAKKFGIQKLSTGDLLRESVDRGTT
                     LGKEAKHFMERGELVPDNVVIGLVEEKLASSECRDGFLLDGFPRTVAQADKLSTFLGG
                     NGEAIDRVVYFSLPESDIIERISGRRSCPKCKAVYHLKSIPPKKNGVCNECEVPLIQR
                     NDDKPETIQSRLVVYQKQTEPLIQYYKDRGILSELDGSGVVSAVQDRLVALLTQSR"
     gene            11632..12378
                     /gene="map"
                     /locus_tag="PKM72_01305"
     CDS             11632..12378
                     /gene="map"
                     /locus_tag="PKM72_01305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNP59441.1"
                     /translation="MIIIKTAEEIELIARAGKIVTHCHQLLVNAVRPGITTLELDRLT
                     EECILEMGGIPAFKGYRNFPNSLCASINEEVVHGIPSRRELKEGDIIGLDVGAIVEGF
                     YGDGAVTVSVGAVPEKIQLLIGVTREALLKGLEKAVVGNRLSDISFAIQTHVENHGYS
                     VVRDFVGHGIGRQLHEEPQVPNYGRPGQGPRLKPGMALAIEPMVNLGGSAVKVLKDGW
                     TAVTCDNSLSAHFEHTITIEANGAPRILTG"
     gene            12409..12627
                     /gene="infA"
                     /locus_tag="PKM72_01310"
     CDS             12409..12627
                     /gene="infA"
                     /locus_tag="PKM72_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNP59442.1"
                     /translation="MGKEDIIEVQGAVTETLPNAMFRVQLENGHRILAHISGKMRMHF
                     IRILPGDKVTVELSPYDLTRGRITYRFK"
     gene            12639..12752
                     /gene="rpmJ"
                     /locus_tag="PKM72_01315"
     CDS             12639..12752
                     /gene="rpmJ"
                     /locus_tag="PKM72_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNP59443.1"
                     /translation="MKVKSSVKPICVKCKVIRRKGVVRVLCTNPKHKQRQG"
     gene            12813..13184
                     /gene="rpsM"
                     /locus_tag="PKM72_01320"
     CDS             12813..13184
                     /gene="rpsM"
                     /locus_tag="PKM72_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNP59444.1"
                     /translation="MARIAGVELPVGKRIDVGLTYIFGIGPASSRDILQKTGVSPETR
                     VKDLEEDEVVRLRETIDKDYDVEGDLRKEITMSIKRLMDIGTYRGLRHRKGLPVRGQR
                     TKTNSRTRKGRRGAIGGKVRK"
     gene            13205..13588
                     /gene="rpsK"
                     /locus_tag="PKM72_01325"
     CDS             13205..13588
                     /gene="rpsK"
                     /locus_tag="PKM72_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNP59445.1"
                     /translation="MSVKKGKKKDRKVLQVGIVHIMASFNNTIVTITDMTGGAVTWAS
                     AGSQGFKGSRKSTPFAATRAGEVAAKKAMEFGLRQVDVYVNGPGSGREAAVRALQSAG
                     LRVNLIRDVTPIPHNGCRPPKRRRV"
     gene            13653..14282
                     /gene="rpsD"
                     /locus_tag="PKM72_01330"
     CDS             13653..14282
                     /gene="rpsD"
                     /locus_tag="PKM72_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNP59446.1"
                     /translation="MAKYTGPVCRLCRREGVKLFLKGTRCMTEKCAIERRSYPPGQHG
                     QSRRGRATEYGTQLREKQKLRRVYGMQERQFLNTYHRAVRRKGITGEHLLSLLERRLD
                     NVVYRLGFASSRGQARQLVNHGHVMLNGRKVTIASVTVNVGDIIEIREKSRQLVPVQA
                     ALEITSGRGVPNWLEMERNLLKGTVQAIPTKQDIDVLVNEQIVVELYSR"
     gene            14377..15351
                     /locus_tag="PKM72_01335"
     CDS             14377..15351
                     /locus_tag="PKM72_01335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNP59447.1"
                     /translation="MKDFQIPTKLELEKDTASPTYGKFTAEPFERGFGTTMGNSLRRV
                     LLSSLPGAAVTSVKIEGVYHEFSTIPGVTEDVTIIILNIKALRLRLHSDKAKAIRLKK
                     KGPGEVLAADLSVDPDVVVLNPDFHIATLDKDGMLDIELIVKPGRGYVPAERNKEEGL
                     PIGVIPVDSVFSPIKRVNFSVESARVGRITDYDKLILEVWTDGSITPQEAVTSAAGIL
                     RNHLDICLPSEERGEPAQEEGGDESKMLINQHLFRSVNELELSVRAANCLKNANIKSI
                     GDLVHKSENEMLKTKNFGKKSLNEIKEVLAEMGLTLGTKVSEVTESQT"
     gene            15375..15773
                     /gene="rplQ"
                     /locus_tag="PKM72_01340"
     CDS             15375..15773
                     /gene="rplQ"
                     /locus_tag="PKM72_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNP59448.1"
                     /translation="MRHRKRGRQLGRNSKHRLALFRNLVTSLMEHERIETTEAKAKEL
                     RGITDRLITLGKQGTLQARRGALRVLRTKQTVQKLFDDVAKRFPERNGGYTRIIKTRQ
                     RPGDAAKLVAIELVEKTNESDKEIVSSTPS"
     gene            15961..16539
                     /gene="lptC"
                     /locus_tag="PKM72_01345"
     CDS             15961..16539
                     /gene="lptC"
                     /locus_tag="PKM72_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNP59449.1"
                     /translation="MQFYGTRAVLTLLVLGMTIFIGSRVINHMQTRSQDTTLVTLQEQ
                     QGADAWIQGFNYRQTRSGSTKWVVNADQAKVFDKEHVAKLETVQVHLFDGEFQKEQLK
                     ITSEKGVMNTSSNDFELISKNEKTVMTFESGYQVFSDKLTWNEEARQIHTPDQVLIKG
                     DGLTITGTGLEGDVDKHEFHLLKNVRAELVSP"
     gene            16548..17063
                     /locus_tag="PKM72_01350"
     CDS             16548..17063
                     /locus_tag="PKM72_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNP59450.1"
                     /translation="MPISFGWLIFAIILVHVPIVAMGEEPKASTVITSKTMTASSEKN
                     VAIFQGAVKMVQGELVVHSDVMIVYFKEKAPSNPSAATDSGAKSESGNEINYIEAKGN
                     VRIEKGESRATSQHALYYKSEEKVILTGSPVAWQTGTRISGPKMTMFLKENRSMVEGG
                     TRVIIDGSEQN"
     gene            17269..18021
                     /gene="lptB"
                     /locus_tag="PKM72_01355"
     CDS             17269..18021
                     /gene="lptB"
                     /locus_tag="PKM72_01355"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNP59451.1"
                     /translation="MPEARGKSLHRISAKDLKKSYKGRQVVRGVSLELLGGEVVGLLG
                     PNGAGKTTIFDMVVGLCQPDQGKIFLNNDEITFLPMYKRARRGIGYLPQEASIFRRLS
                     VEDNVLAVLETLNLPASGRKARLDELLEELDLTRLRRHGSYTLSGGERRRLEITRALA
                     TNPKFLLLDEPFAGIDPIAVGDIQDILTSLKRKNIGILITDHNVQDTLSITDRAYIIS
                     EGTILESGPPDVIVNSPKARAVYLGERFKMMQ"
     gene            18042..19559
                     /gene="rpoN"
                     /locus_tag="PKM72_01360"
     CDS             18042..19559
                     /gene="rpoN"
                     /locus_tag="PKM72_01360"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNP59452.1"
                     /translation="MDLRLDLRLSQKLVMTPQLQQAIKLLQLSRLELQQTLAQHLVEN
                     PLLEDLALEAQDEEPSVGEESVGQVKEPDTESEPSGEVAEVQPTIKEGEPLSSEEWDN
                     ILGNDWRPAQREEAYSGDEEFPSYEQTLTKPTSLEEHLDWQLRLSSLAGDDRDIGRMI
                     IGNLDEDGYMRVPIAELAEDAKCSVEHVEGVLRQIQQFDPPGVAARDLAECLLIQLDH
                     LQHDETELTSHLQAAVPMHVVAAILKDHLVDLQKKRYQSVAKTLSVTLDEVYEAVRVI
                     ESLEPKPGRPYFSDSNSIIIPDVYVVKYEGEWVVLLNDDGLPRLRISPYYRRLLSHSG
                     EDAGNTKNYLEEKLKGAQWIIRSIDQRNKTIVKVVKSLVKFQEAFLEKGIQYLRPLVL
                     KQVAEDVGMHESTISRVTTNKYLHCPQGILELKFFFNAGIQRADNRGEDMSSVTVREM
                     IREMVAQEDAANPLKDQEIVSRLRHKNILIARRTVAKYRAELHISSASQRKRIPT"
     gene            19665..20609
                     /gene="rapZ"
                     /locus_tag="PKM72_01365"
     CDS             19665..20609
                     /gene="rapZ"
                     /locus_tag="PKM72_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNP59453.1"
                     /translation="MHDDCGSETMPSESPQASLKNDPLPLQLMMVTGTSGSGKTQALK
                     CLEDLGFFCVDNLPPALFQKFTELCIQPDKDVRKVALGIDIRERAFFEDLLSNLEALQ
                     SDGCHVELLFLEAHDDILVRRFSESRRPHPLLPHRPIIEGIQLERERLQSLRARAHRV
                     LDTSEFSVHDLKAWIIKHYRERGEGQVLKLSLMSFGYKFGVPFDVDMVFDVRFLRNPH
                     FVPDLQPLTGEHGDIQRFVLDTREAKVFLDHLKELFAFLLPMFEREQRSYVTVAFGCT
                     GGRHRSVAMVLRMKEILNILGYDVTIHHREISKEANPG"
     gene            20954..22024
                     /gene="pilM"
                     /locus_tag="PKM72_01370"
     CDS             20954..22024
                     /gene="pilM"
                     /locus_tag="PKM72_01370"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNP59454.1"
                     /translation="MNAIWKGIAERVSGYSWKSQNVTGVDVGSRLLKAVTLKPRYGSP
                     FLQGVVIETLTSRSNLDPQSDFEQDRHLVQILRKQLSPSPRTVGLTVSSSHVLLKRLT
                     FPHMSENDLREHLALELDRYIPLDVQGAVWDVYRHNVTGPLDNGKDQALLVVAKKEFI
                     EERMRQFQQQGMSVGFVDVDAFSLVNMVVYNYGQEGTWLIVHLGPTGILSVVMEEGHP
                     IHIHQVSYEADWYGDLLDGMQSASETSGNGYKIGASESLLLEQFFKETIEQVIQTAKD
                     VSEITDGGVVRGVFLSGGYSVLEGLQSRLADSLAVSVTPVNPFKKIAVPQDIQQDCRF
                     QKALPLFGVAVGAALRWLDPHD"
     gene            22017..22580
                     /locus_tag="PKM72_01375"
     CDS             22017..22580
                     /locus_tag="PKM72_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59455.1"
                     /translation="MIEINLARQIQRQVSPHKSSWLICMLVGLVVFMGFGGASWWWTK
                     SLQEQVDALLQEKSVKMQALVRIKERLQNVESRKEQKALLMTSVGRLSDQEKEKAWPV
                     ALLDGVSRNVGDLDIWLERVQLESRMVELHGQSLALEDIVEFMEALENDRIIMSLPVV
                     EILERPEGESVAFSFLIRFVWDHQVTT"
     gene            22577..23170
                     /gene="pilO"
                     /locus_tag="PKM72_01380"
     CDS             22577..23170
                     /gene="pilO"
                     /locus_tag="PKM72_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNP59456.1"
                     /translation="MMAQWKALSIWHRFGFLVGVMLVCLLGNHLFVWQQMSHSLEELQ
                     QEVAHLDQERHGLLQKTDSLKAIEREINVLREILSARIQQLPEHIESKVFRRDIMEIA
                     KRRGVTVRGWKPEGLWTELQGAEASIRIAVRVEGEFEGTVQFLDDLRQLAWVESISSI
                     VMAGRSENENSSTLITNLVMHGLTPLGIEHVQQLLKT"
     gene            23145..23762
                     /locus_tag="PKM72_01385"
     CDS             23145..23762
                     /locus_tag="PKM72_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59457.1"
                     /translation="MFSNCLKHDRANQLLAGMRGHGVMFMWGTVSRMAVYWRWCASYS
                     VCVWGVLIFVGLMGEAAGKGASPLPSHPSRLAKTSVDQDNARHAVVNRRDPFKRIPKP
                     LPTPKVSTQSDTEPKDAPLADNPVWRLLGVMHGQDGHQAVIQISPTERVLVQPGVELA
                     RSGWTIKTISEKEVLLEYVSSASSVRGASPTRTFILSFPTIQKSP"
     gene            23980..25140
                     /gene="aroC"
                     /locus_tag="PKM72_01390"
     CDS             23980..25140
                     /gene="aroC"
                     /locus_tag="PKM72_01390"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNP59458.1"
                     /translation="MLRYLNAGESHGRGLMAVVEGVPSGLPVVADEINADLIRRQGGY
                     GRGGRMRIEKDRIEFICGVRKGKTLGNPLGLLIWNKDWENWKDIMASEPGPPSTERVV
                     TRPRPGHADLVGAIKYGHRDIRNVLEKASARETAIRVAIGGVAKAVLAQFDMRVVSYT
                     MDIGGVAAPSPNDPLMAYEYAEQSDVRCHDPETAKKMIEQIRAAKHKGDSLGGIFEVV
                     VTNVPIGLGTYAQWDRRLSARLAFAAMSIQAMKGVEIGMGFESARRFGSEVHDDIYFD
                     KATGQFIRKSNNAGGLEGGITNGQPIVLRVAMKPIATLYTPKDSVDIETKESFEATVE
                     RSDICTVPAAGVVGEAVIAYEIANALIEKFGGDTLDEMKKNFEAYQEYVRTF"
     gene            25220..26389
                     /gene="aroB"
                     /locus_tag="PKM72_01395"
     CDS             25220..26389
                     /gene="aroB"
                     /locus_tag="PKM72_01395"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNP59459.1"
                     /translation="MNISELSKHVPVSLGDRSYHVLIQPQILPQIGRVLQDVGLSGQV
                     AIVTNSIVNELYGRVVFRSLKRCGFSPFFVVIPDGEKAKSMYWLSKILDELVRQRLER
                     QEVVLALGGGVVGDVAGFAASVYLRGVPFVQVPTTLVAQVDSSVGGKTGVNHPIGKNL
                     VGAFYQPRVVVVDPQALQSLPKREWVAGLAEVIKYGMIADPKFFEYLEQNIDGLREQA
                     EDVIPTVIRRCCEIKAEVVGGDERESGRRRILNYGHTVGHALETWGRYKTWIHGEAVG
                     IGMVQEAAMAQFLGMCTNELVERQRDLIQDVGLPIAMPSMTFMDLWGAMQHDKKVVKG
                     QINCVVPTSIGNVQVVPLVRQRIRQWFTASQSSRVKRLKSVPETLSRPRLSRKQK"
     gene            26391..27644
                     /locus_tag="PKM72_01400"
     CDS             26391..27644
                     /locus_tag="PKM72_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNP59460.1"
                     /translation="MAQVRPSTIEDLKHLVRERSREVQILHRISESISGSLELETVLK
                     QIVEVVVEVTKGDACLLYLISETTDELILRASKNPHPRLIGRISIGLGEGITGWVARE
                     KVPVVISRNASDDARFKFFHRLPEDRFQAFVSVPITSKGRMIGVINVQHKRPKRYAEE
                     TLALLLTIANQVGGAIANARLYDDMRQKARRLETLSQVSETVASNRLIEDVLQLIVTM
                     TAQLMNSKICSIMLVDQASGELRIAATQSLSDAYRRKPPLKVGQSMSGRAVQDHHPVY
                     VSDVKAEEGYFYRDLAKQEGLHSLLSIPMLMKENPIGVINVYTSSYHAFSDEEVITLQ
                     AIANQSAVAIEHTRLMEKSFEMQEALEVRKLVERAKGFLMASRGLSEPEAFRLMQRQS
                     MNLRKSMREIADAIILAEELQRATQ"
     gene            27819..29609
                     /locus_tag="PKM72_01405"
     CDS             27819..29609
                     /locus_tag="PKM72_01405"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNP59461.1"
                     /translation="MMLRLAMVQMNAVVGDLEGNTRTICGWIREAKKAKADVVVFPEL
                     AVCGYPPEDLLLMPQFLFNISRMRDRIAKVTKGIMAVVGSVIESDRPAQHSILGSGLD
                     RRKPLNAAWVLVDGQVKRSYAKSKLPNYGVFDEERYFQPGRTSPVYCLGPLRIGINIC
                     EDIWFANGPASQQAAYGGAQLILNINASPYHIGKTQSREHMLAVRAKANNVMVSYTNM
                     VGGQDELVFDGNSLVMDSTGRILARAKAFKEDFLLTDLNIPSIRKGKFKRSGKEPEFD
                     ADEVRIPMIRMGWTPTWPKSGVPSVIRLTPVIGEMEEIYRALVLGVQDYVRKNAFSRV
                     LVGISGGIDSALTALIARDALGASNVMGVMMPSPYTSKESREDARRLGKKLGIDMRSL
                     SISGVFKTFLRVLGKSFHGCQADTTEENLQARIRGTLLMALSNKFGYLVLTTGNKSEM
                     SVGYATLYGDMAGGFAVIKDIPKTKVYQLSRWRSEYRGSCFDRVIIPERIQVRAPSAE
                     LRPDQTDQDALPPYPVLDAILQAYVEDNESMNRIVKMGYDSHVVRMVMGMVDRSEYKR
                     RQAPVGIKITSRALGKDRRMPITNRYVSGD"
     gene            29652..32357
                     /gene="glnD"
                     /locus_tag="PKM72_01410"
     CDS             29652..32357
                     /gene="glnD"
                     /locus_tag="PKM72_01410"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNP59462.1"
                     /translation="MSEDLPLTPPGLTEHKDSYLGLHLLQEQREVLRQRLIEGAMGSE
                     ITQAFSDFMDALLIARFREVIQQGNAGVRAGWQQCCLVAMGGYGRRELAPYSDIDVMI
                     LTQGNQGEVAEALSTGVFHRLWDLGFQVGHSVRSLAECLTIAEADLAACTSLMESRFL
                     IGSASVFQEFQRKFARRIIKKRAQRFVLDKIQEREREYAKFGETVFMLEPNIKKSKGG
                     LRDLHLLQWIGQARYGAGTIQELSNRGIIAIQDYQVLQEAQEFLWRVRCFLHFEAGRA
                     QDILTFDDQVRLSALWGFVDHPHLLAVEQFMQVYFRHTTGLFDRGRRFLDQAKKGTWL
                     DRVRRWCPSPLIEGHFRILENRLTIQTDKLLEVMESPMLLLKLFMVSHRQAVPIDSCI
                     LNELSQHMGTLPNEQFHIKSVSGLFRHILSTPGRIAQTLEAMHQAGLLEKLVPAFSRV
                     RGLMQFNQYHKYTVDEHSLLAVREAERLQNHQGIIGEVYRNIPDKDILHLALLLHDLG
                     KGRPGDHSEVGQEIAHLTADRLDLSEKERQALAFLVRHHLQMSHTAFRRDLNDPKTIV
                     TFARIVKTVGILQKLFILTIADISAVGPEVMTKWKESLLGELYSLTYAELAGDTGPSN
                     SGDGSMNFSPEARNELIGILKNYRGEGRYPEFDWTERVNATLQQLPGRYVQSTPVGQM
                     AAYLLAIQSLPVKSTQVEARFNQTLGVSEYVLITREQTKPGIFMQVAGVLATMGLQVL
                     EAQIMTLKDGTVWDVFSVYDSDYEGPPSVGRLEEVAKEIQQVVEGRQSVHQLVERRRR
                     MAFGRPFPTGRHPTEVHIDQGVSDAYTVIDIFADDKQGLLFVIAKTLVQLDLSIHMAR
                     IGTRLDQVADVFYVTNSHGEKILSVENCEQIQHTLHAAVDQFLDE"
     assembly_gap    32643..32742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33466..34638)
                     /locus_tag="PKM72_01415"
     CDS             complement(33466..34638)
                     /locus_tag="PKM72_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNP59463.1"
                     /translation="MMIAPTPTVPVVSKTPFLSVHQWLFLILCLMVGMYAGLGHAPSQ
                     WTDALPDIVLLGATGLAIGCIGLLIERQLMSCRRSTLIGGSIGLIVALAGIGFLSIMG
                     MGSGLLQMSSLTPWIGLPLYLLCPYIGLIVGIHISNTLVPAPIAEQPKNSSPHTSLTP
                     SNTVQKLLDSSAIIDGRILPLCTTGFLEGPFLVPKSILHELQTLADSAHPSKRIKGKR
                     GLDILSQLQQLPNMEVVIIEDWEPDISAVDHQLIAIAKNREAKIVTNDWNLAKVALLQ
                     GVSSLNVNELTYQLRPLVLPGETIRVFIHKEGQGQGQGIAHLDDGTMVVVDHGATLVG
                     QAIEVVVTRFMQTSTGRMIFSTPQTNTSSMLFNLQPLPNRSEAAQEYSRSLVEDQG"
     gene            complement(34690..35220)
                     /locus_tag="PKM72_01420"
     CDS             complement(34690..35220)
                     /locus_tag="PKM72_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59464.1"
                     /translation="MKIGTRAPMWKTPCLLKGGLQYLPLTAFQGSQFVLCCLPAFTET
                     DAWLLEQQADRFHASGAVLAALISDKALLGNSWCRPPRDFGLPLLTDPLRRLERSLHL
                     TNSLPPHRCETLFFDHRCYLQFRLFHDLNLRGITTVLEVAESDFCQRSAQPVLKSRLT
                     FDHSLAHCLPLEAPTG"
     gene            complement(36274..36753)
                     /locus_tag="PKM72_01425"
     CDS             complement(36274..36753)
                     /locus_tag="PKM72_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59465.1"
                     /translation="MGKRLLQVCVSILIYWGGQAQIVHSVQSGTPDHPIAWEPLPNGL
                     ISMAYDRTGDGIPDHFTLHPITWSGWSAQAIEEIEHQACMDDQWVFMVEYAQDRYVYF
                     AQQTPVLVGDDPKQTGRWMAQNVRPTDVKEYQHLNQTAHQPDNVTCPIAVPRQHQKP"
     gene            complement(36768..36902)
                     /locus_tag="PKM72_01430"
     CDS             complement(36768..36902)
                     /locus_tag="PKM72_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59466.1"
                     /translation="MNGHSFIVEGEKPVFISLLISLGLVFLSIIGLWAWPEFMMAIGH
                     "
     gene            37452..37928
                     /gene="bfr"
                     /locus_tag="PKM72_01435"
     CDS             37452..37928
                     /gene="bfr"
                     /locus_tag="PKM72_01435"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNP59467.1"
                     /translation="MKAKKGVVEFLNQILTEELTVINQYFLHAKMCDNWGYNRLEHKV
                     KERSFSEMKDADKIIEHILYLEGVPNVQRLGTVKIGESVPEQLKSDLAKEKKMVTLLT
                     EAIQHCTKVGDFTTRHLLEDMVADEDEHIDWIETQQETIKQIGLENYLSEQIKNEG"
     gene            complement(38599..38805)
                     /locus_tag="PKM72_01440"
     CDS             complement(38599..38805)
                     /locus_tag="PKM72_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59468.1"
                     /translation="MPPSQQLKLNVCDCSRIHLTYKSVTLHFEKEEFLAYAMQMTRMA
                     AQVSNTAGLRRAPDLVDAAISNCH"
     gene            complement(38848..39384)
                     /locus_tag="PKM72_01445"
     CDS             complement(38848..39384)
                     /locus_tag="PKM72_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNP59469.1"
                     /translation="MTIGHSTIPRMRVPALLKGNMIDVDLASLEGQWGMLCCLPRLEF
                     CDAVFLNQFRRAVQKQGAALLGIQHPAHPFLAAHLPKAKILSIPLLTDSAQRIGRALG
                     LSSETHLNQCQSFIFDPEGVVRNKLTHSFNWHCMTLLLGILKQCQVFYPQATQEHIDF
                     PPTDNFIFAEQGLNPSLC"
     gene            complement(39526..40074)
                     /locus_tag="PKM72_01450"
     CDS             complement(39526..40074)
                     /locus_tag="PKM72_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNP59470.1"
                     /translation="MTCQREQQNSQDHLEKLIEELRCLAREGTHVKIGTNPVEDRSSF
                     ICKILQDLLSGLKDTRAFSHSTAQQIHTFMISHLHQGLTLKDISLFLGYSEKYCSQLF
                     HTVIGESFRFHLKRLRIERAKFLLQEGHANLREIATALGFSDQFAFSHFFKNAVGYSP
                     SCFRALFEDVSEVQPTQNANER"
     gene            complement(40720..40983)
                     /locus_tag="PKM72_01455"
     CDS             complement(40720..40983)
                     /locus_tag="PKM72_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59471.1"
                     /translation="MEEKMILRGFTPDQKREMLPTENCYMSSSAVYGPKNSMAKSSPR
                     LGVRLMEGIALRDPGKEFLVVQRVQRLDEGAHRACAMISGYGH"
     gene            complement(41327..43165)
                     /locus_tag="PKM72_01460"
     CDS             complement(41327..43165)
                     /locus_tag="PKM72_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59472.1"
                     /translation="MNIRRVIIFSCAQVFCISLLTSTLSFLVNAPAVLAQEAEEADPP
                     EVTNGERLFLETRFAQLFKNFLDDGGDVNDPLTQGDPALDKVANWQLPPDQYADGPFA
                     GQSMNCRSCHFVDEVLDIPKYGMNTYSDFARRSPIPAREDEKHTSVRNAPPLVNAALP
                     RQMFFLHTDAEFPTMVDLVKGTLTGRNYGWLPNEEAAAISHIARIIREDSGTGDLAQE
                     FGGLSYRVLFTGTDPSIPQEFLIPEDFRVEVSQATDLQLVQAVTQLIAAYTDQLLFSQ
                     DDEGNYNLSPYDAFLEINDLPRQPDKWESPDSYTRRLLQQIQFLESYDSQNYSRWPSP
                     SSSDSPTLQFVEKNPHTADGKFQFHNQPFTFGPEELKGLKIFFAQQDRHRHRRHKGYK
                     IRPSDQAKGGIGNCVACHTPPNFTDFRFHNTGIAQTEYERIHGPESFSMLQIPRLDER
                     NGNIEAYLPATGKHPYAQEPFRAIPSPDNPQQTDLGIWNIFWNPDFPGSQLPIWSILC
                     EDTLNEHYGVFRGFQACRPDRLLPKAIGAFKTPGLRDLGHSAPYSHTGIADTLEDVIR
                     GYMTNSDLMRNGKLRNGASQLKDIVLLPEDIPALTAFLRSLNEDYE"
BASE COUNT        11882 a         8492 c        11667 g        11655 t
ORIGIN      
        1 ggcggcatac gccgtttttt aatgggtatc gtccgcagtt ttacttccgg acgacggatg
       61 tcacgggggt ggtgcaatta aacccggggg tggagatggt gatgccgggg gataatgtgt
      121 cgtttgaggg ggaattgatc tccccgattg ccatggagca aggggtgcga tttgcagtgc
      181 gagaaggggg ccgaacggtg ggagccgggg tggtcacgga aatcgtcgcg taagaacata
      241 ctggtgttag tggaaagggt aggtttttgt ggatcgagac cgcagaatac gaatacgctt
      301 aaaaggtttt gactataggg tgttggatca atctcttcgt gagattgttg atactgttcg
      361 tcggagcgga gcccgcattg ccggaccggt gcctctccct acgaggattg agaagtggac
      421 ggttcagaga tcgactcacg ttgataaaaa gtcacgtgag cagtttgaaa tccgaactca
      481 taagcggttg ctggacatta tggaaccaac gcctgagact atggacgctt tgatgaagct
      541 gaatctggcg gccggagtag atgtggaaat taagctttag gttttgtggt caaggctttc
      601 cttagttacg gtaaacttaa tctaggtaat tattgaatta tggttaaagg gttgatagga
      661 aaaaaattag gaatgattca agtgttcgat gaggagcggc gccttatccc tgtgacggtt
      721 attgaagcgg gtccatgcgg aattgttcag ctcaaaaaaa agagtacaga tggctatgaa
      781 gccgtccaaa taggattcga tgaggtcccg gagcggaagc aatcaaaggc ccagttgggg
      841 cacctgaaaa aagcaggaaa taaggtttgg cgtcatttaa gggaatttga ctctgccggg
      901 gatggggagg ttggctccat ggttgacgta aatttattta ccgaaggtga gccaattaat
      961 gtccagggga tttctaaggg aaagggtttt cagggagtta ttaaaaggca taattatgct
     1021 ggtggtcccg cgtcccatgg ttcaatgttc catcgagccc ctgggtctat cggatcaagc
     1081 tccttccctt caagggtgtt aaagaataaa aagctacctg gtcatatggg taataagcag
     1141 ataacggtta gaggtttgaa ggttttcgga ataaagtcag aagaaaatat cctgttaatt
     1201 accgggtctg tccctggtcc ggtcggaggg gtagtcattg taaggaaggt tggctaatgc
     1261 agtctattga tgcttttccc gtaataggcc aagacgctcg gcctgtagat aaagttgact
     1321 taactgatgg ggttttcagt tccactgtca atccttcgct ggttcatcgg gctgtggtga
     1381 tgcagcaatc aagtaaaagg cagggtacgg cttctacaat cgggcgtggc gaagtaaggg
     1441 gtggtggtaa aaaaccttgg aagcagaaac atacggggag agcaaggtct ggatcaagtc
     1501 gttctcccat ttggaggggt ggtgggaccg tttttgggcc acaaccacga tcatatgctt
     1561 ccacgctgcc aaaaaaaatg tatagggcgg ccctgaaggg ggctcttgca tccaaggttg
     1621 gagatggatt gatagttttg gatgatctgg cactgccaga aatgaaaaca cgtgaattgg
     1681 ttaaattgtt attaaatatt ggggcaagtg gaaaagtcct tttagttatc catgatggtt
     1741 tggccgattt ttttcgtctt gggaaaaatg taaaaaattt aaagatcctt catggcaaag
     1801 accttaatgt gtatgacgtg ctttggtgtg agaaaatggt agtaaccaga gtagagctag
     1861 aacgaatcca ggaaatttgg gtgtaacgtg aatccacatg atgttttgat acgtccattg
     1921 ttaacagaaa aaatcacggc aatacgcgaa ataaaaaacg ctattgcatt ctccgtgcat
     1981 cgtcaggcaa cgcgaattga tgttcgtaga gccgtggaga aggtttttag cgtaaaggtc
     2041 gcttctgtga atatgatgaa tgttaagggc aaaaagaagc gacaaggtcg ttttaccggt
     2101 aaaagatcgg attggagaaa ggccttcatc acattaaaag aaggtgaaaa aatagaactg
     2161 tatgaaagcg cctagtatgg tttctttgtt tcatttaatg aatggtgaga attaggttat
     2221 gccaataaaa gaatataatc caacatcacc tgggcgaaga ggaatgtcgg cagttacggg
     2281 tgaggggttg tcgcgtaaaa ggcctctgaa atccttgacc acctctaatt ctaagtctgg
     2341 tggaaggaat aacgctggga ggattacatc ccgatttaag ggtggtggac ataagcggct
     2401 ttatcggaaa attgatttca aacgtgacaa gtttaaggtt gtcggtaagg ttgccggttt
     2461 ggagtatgac ccaaatcgtt ccgctcgaat tgcactgctg tattatgcag atggagagaa
     2521 gcgctatatt ctggctcctt ttggtttaaa ggtgggtgat tcagtccagt ccggtagtgg
     2581 agttgacgtg agggttggga atgcgatgcc acttatggaa atgccgcttg gtatagtggt
     2641 tcataatgtt gaattgaagc cagggaaggg tgcccagttg gctagaagtg ctggggcttc
     2701 tgctcaggta atgggccgtg atgaaggtta cgtccagatt agattaacgt ctggtgaaat
     2761 gagaaagata ctcggaacat gtatggcaac tattggccag gtgggaaata cggaccataa
     2821 caacgtaact attggcaaag ccggcaggtc acgatggttg ggacgtaagc ctcatcagcg
     2881 tggggttgtg atgaatccgg ttgaccatcc gcatggcggt ggggagggta agtctggtca
     2941 aggtaatcct catcccgttt caccgtgggg gcagcctacc aagggtttta agactcggaa
     3001 gccgcgaaat caaagctcgc gttttatcat tgcaggtaga aagaaaaagt cccgttaatt
     3061 taagggggaa tcgatgccaa ggtcagttca taaaggtcca tttgtcgatg accatattgc
     3121 taaaaaagta gaaaaggcca gggaagggag agagtccaag gtaataaaaa cttggtctcg
     3181 ccgttcgaca attattccgg atatgatcgg tttgactttt gcggtccata atgggaaaaa
     3241 atttattccg gtttttatta ccgacaatat ggttggccat aaattgggtg aattcgctcc
     3301 aacaagattt tttaaaggac atgggcatgc caggagtgag aaggcagttg cgctaaagta
     3361 gggaaattaa ccgaatccga agtcatgaaa tacttgagaa ggtagaataa agatatgact
     3421 gaagctaaag caattcttcg acatgtaaga ctggccccac gtaaagcaag gttaatggtt
     3481 gatttggtga ggggaaggaa tgcggcggaa gcattggcca tattgaaata tacgccaagg
     3541 tcagctgcca gggtggttga acaattgctc ttttctgcag tttccaacgc tgggcaaaaa
     3601 gatttgggtg acccggaaaa tcttaaaatt tcgagggctt atgtggatgg tgctcaaatt
     3661 tacaaacggt ttcgcccgcg atcaatggga agggctaacc ctattcataa gcgaacaagt
     3721 catattacga tcgttgtatg tccagctaat tgataaagtg cctttatatt caaaacttgg
     3781 gagttagggg gagacagaat acatgggtca aaagacgcac ccgtatggat tcagattagg
     3841 ttatacccgt acatggcatt cgcgatggta tgcaaaaaag gactatgcga agcttcttca
     3901 ccaagatttg tctataaggg acgtggttaa aaagcgattg tatcatgccg gcatatcaag
     3961 tgtagagatt gaacggtcag gtaaccagct gaaattaatt attcataccg cccgtcctgg
     4021 aattatcata gggaggaagg gcgctgaagt agataaattg aaggccacgc ttgaaaaaga
     4081 atatggcaat gaagttttcg taaacgtgaa ggaaattaaa aagccggaat tggatgcgca
     4141 gttggttgcg gaaaatattg ctacccaatt ggaaaagcgt gtctcttttc ggcgtgcatt
     4201 gaagcgtagt gttgcatctt cccttcgact tggggctttg ggaattaaag tgtactgtgc
     4261 tggacgcctg ggtggacatg agattgccag aacggaatgg tatcgggagg gtcgagtacc
     4321 ccttcatact cttagggccg acgtggaata tggatttgca gaagcaaaaa ccgcaatggg
     4381 tcaaatagga gttaaggctt ggatttataa aggggatgct cagattcctt ctttggaaaa
     4441 atccgagcct tccttgggtt ttttataaaa gattaaaaga tttaagtgat tggagtaatt
     4501 gaaagatgtt agctccgaaa agggtaaaat tcagaaaggt tcaaaaaggc cgaatgcgtg
     4561 ggaaggccta ccgtgggggg gaaatcgcct acggggtgtt tggccttaaa gccatggagc
     4621 ccggaagaat tactgccagg caaatcgaag cagctcgtat tgctatgacc agacatgtaa
     4681 aaaggggtgg aaggatttgg accagaattt tccctgataa acccattacg aagaaacctg
     4741 cggaagttcg aatgggaaaa ggaaagggta accctgagta ttgggttgca cctataaagt
     4801 ctggaagaat tttgtttgaa atggatgatg tttcccaaat cgttgcggaa gaggctttgc
     4861 gtttagcagg acataagtta cctgtggcaa caaagtttgt tattcgtggc gaaataccgg
     4921 ttttgtaggt attggatatg gaaatggacg aaataaaaaa tttagaaaaa attgaattgc
     4981 ttgaaaaagt aaataaattg aaacaagaat tgtttcattt taggagtcaa ttggcattgg
     5041 ggcgaatgga aaaccccatg agaatacgtg agacaagaaa aaatatcgcc agggttcaaa
     5101 cggttcttcg ccaaaaagct acctagtcaa ccacgatttt gaaggaaagt tgctccacta
     5161 tgacacagac cagggctatg cctcgatcgc ttacgggaaa agttgtcagt aataaaatgc
     5221 aaaagaccgt agtggttgaa gttgttcgca ttgagcgcca ttctttgtat gggaaagtta
     5281 tgagaaaaaa aaccaagata aaagctcatg acgaaataaa ccaatgccta ataggtgatc
     5341 aggttaatat tgtttatgcg cgccctttaa gcaagagcaa gcactggcgt gttgcagagg
     5401 tcttaactaa gcgagtgacc acctaggatt acagaagaag gtgtcttata ttttcccttg
     5461 agtttgtaag aaatagagtt aataaatgat acaaaattat acctatcttg atgttgctga
     5521 taattcaggc gcaaaaaatg tcatgtgctt tcatgtattg ggtggtacaa agcgtcggta
     5581 tggatctttg ggtgacatta ttgttgtggc agtgaaagag gcaattccaa aggcatccgt
     5641 taaaaagggt gacgtgatga aggctgtgat tgtgcgaaca aaaaaaggcc gacgtcgaga
     5701 ggatggttca cacattaaat ttgaccggaa tgcttgtgtc ttagttaacg ctcagggaga
     5761 cccggttgga actcgtatct ttggaccggt tgcaagagag ttaagaaaga agaaatatat
     5821 gaaaatcata tctctggccc cagaagtcat ttaggtaggt tattatgtct gaaaaatttc
     5881 gaataaaaaa aggcgatatg gttatggtta tcgctggcaa ggagcgtggg aagacgggta
     5941 aggtcctcca ggtccaaaca aaagatgcca gagtcacagt tgaaaagctc aacatattaa
     6001 aacgtcatac aaagcccaac gcaaaaaaca aacagggcgg aattcttgaa agggagggtt
     6061 ttatagccat ttcaaacgtc atggctttct gtgaatccgc caagaaacct tctaggatta
     6121 aaatgaaaac ttttgatgat ggacgaaaag tgcgtgttta ccaaaaagat tcaaacgaag
     6181 ttttggaaaa aagctgatga aatcaaaatc ggttacgaaa caaacagcta aaacaaaaga
     6241 aaaagctgta ggttcttctg ctattaaacc gacggattat gttcctagga ttaaggcgat
     6301 gtaccaagaa aaagtcattc cggctatgat gaaggaattt gggtatcaaa atcccatgca
     6361 ggtccctcgg gttgagcgga ttgtcttaaa tattggaatg ggagaagccg ttcaaaatgt
     6421 aaagttgtta gaaagtgctg ctgcagaact tgagcaaatt tcaggacaaa agcctgtcct
     6481 gactcgttca aagaaggcca tagctggatt taaattgcgt gaaggaattc cgatcggaac
     6541 caaggttaca ttaaggggcg caagaatgca tgagttcttg gaccgtttgg tatccgtggc
     6601 tttgccgaga attcgtgatt tccgtgggat ctcaccaaaa gctttcgacg gacgagggaa
     6661 ctatactctg ggtttaaaag aacagctggc atttcctgaa attaaatatg acgatgttgc
     6721 atcaatacat ggtatggata ttacgtttgt taccaccgct caacggaatg atgaagccaa
     6781 atctttattg acccacttgg gaatgccttt tagaaaaccc tagagaggga aaggagcgta
     6841 cgtgtcaagg ttagcattaa agaataaggc taagcgaaag ccaaaattca cagttcgcca
     6901 ctataatcgc tgcgctcttt gcggcagagt gagaggtttt ttgcgacgtt tccttatgtg
     6961 tcgaatttgt tttcgtttcc ttagtttgaa gggtgatatc cctggtgtca ctaaatctag
     7021 ttggtagact ttttgtggta aacagatttt ttgggtattt ggctaatcat tcaatacgag
     7081 gttttttagc atgtcgatga ctgatcctct cgcagattta ttcgtgcgaa ttcaaaatgc
     7141 gggacgccgt ggacacgatt tggttaaaat cccatcttct cgtgttaaaa aagatatact
     7201 acgaattttc aaggaagaag gatttattcg tgactacaag gcagctacgg gtgccaatgg
     7261 ccacccggtt attgaagtat cgcttcgata tgcccctggt agacaaaaga agtccttcat
     7321 aactgggata aaaagaataa gtaagcctgg atgtagagtt tatgctggga aagaagatct
     7381 gccacgggta atgcgtggat tgggtttggc aatcttgacc actgaccgtg ggttattaac
     7441 ggatgagcaa tgccggagtt cccaggttgg cggtgaaatt ctgtgccata tttggtaatt
     7501 tttggatact tatttacttg gttttttaat taataggtaa atcatgtcga ggattggaag
     7561 aaagccgata tcaattccaa agggcgtgga agtgcgcgtc cttgatggaa acgttttggt
     7621 gaagggaccg ctcggtgagt tgcaatggaa cctaaaccca ggaatacaaa tcagtatcga
     7681 aaatgaggaa gtttccttaa cccggaatag cgattcggca aaactcaagg ccttgcatgg
     7741 actaacaagg gctgaactcg caaatcaggt aaagggggtg aaggagggtt ttcaggaaaa
     7801 cctagaagtg atgggggttg gataccgtgc ccaaattcaa ggaaaagaat tgagcttttc
     7861 cgtaggatat gctcatcccg tatcaatggg attgccaaaa ggggtagagg cgtctgttga
     7921 caaacaaact tcgatttctc ttaagggtat tgataaacga atggttagct tggttgcagc
     7981 tcaaatcaga ggtatgaagg ttcctgatgt gtacaagcaa aaaggtataa agtatgttgg
     8041 tgaaatactt cgtaaaaaag ctggcaaagc cggtaaaaag taggttgaac ttgtgaatat
     8101 acttgaaaaa caaaaaagat taagtaaacg aagtaggcgc gtccgtttgc gcatcgtggg
     8161 cttgtcttcc cgtccacgat taagtgtatt tagaagtaat tctcatatct atgcccagat
     8221 cattgatgat tcggctgggc gcactttggt ttctgcgtca tcctgcgacc ctggtttaaa
     8281 agacaagata aaatctggtg ggaatattga agcggcgaaa atggttggac aagcaatcgc
     8341 tgaacgggca aaagccatac caattgaact agtcgtcttt gatagaggtg gaagattgta
     8401 tcatggtcgg gtaaaggctt tggcagatgc cgcgcgttcc ggaggactga aattctaatc
     8461 agaaccagcc ttaaattttg ctaaagggaa ggattcactt ctgtgagggt taatgttgaa
     8521 gagctcaatt taaaagataa gccggttgta attaaccgag tggccaaggt agtgaaaggt
     8581 ggaaaacgct tttcattttc ggccttggtg gttgtgggtg atggtgatgg ttgggtgggt
     8641 gtgggaaaag gtaaagcgac tgaagttcca tctgctatag ctaaagccgt ggctcatgca
     8701 aaaaaaaatc ttatcagaat accccttaaa tcgaatacca taccccatga ggtgcatgga
     8761 tattttggtg gggaacacat tgtcttaaaa cctgccaagc agggtactgg gatcattgca
     8821 ggaggagcag ttcgctcatt gttggaagtg gctggtgctc agaatatcgt tgcgaaaacc
     8881 ctaggtcgtg ggaatccttt taacgcagtg agagcaacca ttcaagggtt gagtcaactc
     8941 cgtgatccac atgaagtaag agagttgcgt cgaaccgctg tcgggatgga agattaatag
     9001 atatcgaaat gaccagtcag gaaaccagga aatcatggtg acaccaacca atctctcaat
     9061 tacattaaag cgaagcacaa taggccatcc agataagatg cgtgtggttt tattggggct
     9121 tggacttagg aaaattggac agacggttag tcgtccggat acagcccaag tacggggttt
     9181 aatttataaa gtcaggcatc ttattgaggt tgcaggttca tgaagctaca cgatttacat
     9241 ccgtctccag gatcaagaac caagaggaaa cgccttggac gtgggcctgg ttcaggtttg
     9301 ggaaagacat ctggaaaagg gcataagggg ttgttggccc gttcaggtcg ggctaatcaa
     9361 gctggttttg agggagggca aatgccgctt gcccgaagat tacccaaacg tgggtttaca
     9421 aacattttcc ggattcaata ttccgtaata aatcttaaag atttggttgc tcaggaaaaa
     9481 actacaaact ttaacccaac cgtatttaat gaaattgggt taactaaagg ccgaaaccaa
     9541 caaattaaaa tacttggatc tggtgaagtc gaccgtccta tagttgttga agcacataaa
     9601 tttagtgatt ctgccaaaca aaaaattgag aatgccgggg ggcaagtaaa ggtgattggc
     9661 cgtgcttgag cggcttgtca cgagcattca aaatattttc aaaattccgg agttgcgtag
     9721 ccgggtaatt ttcaccttgt ctatgcttgc agtatatcga atcggggctc atgtccccac
     9781 acccggaatt aataatgaag aactttttaa gtttcttact gaacgaggcg gagccctcat
     9841 ggggttcctt gatatttttt ctggaggcgc attatccaga ttgacgattt tcgccttggg
     9901 cataatgccc tatattagcg cctcaattat tttacagctc cttacggtcg ttgttccgca
     9961 tctttcgaaa ttagcaaaag aaggagaacg aggaagaaag acgatcattc agtataccag
    10021 gtatggcacg atccttattg ccctaattca aggatttggt attgctcttg gccttgaagg
    10081 catgaatgat ggggcatttg ttcttgaccc tggttggcct tttcgattga tgacagtgat
    10141 tacgttggta gccggaacgg cctttcttat gtggttagga gaacagatta ccgaacgcgg
    10201 ggtgggaaac ggaatctccc ttataatttt ttcaggtatc gttgctagtc ttccaagtgc
    10261 ggtggttcaa acatttgatc tatatcaggt tggtcaaatc agcctcctct tactgctaat
    10321 ccttggggtc gtgatgttgg cagtaatggg agctattgtg tttctggaaa gcggacgaag
    10381 aaaaattgcg gttcaatatg ccaagcgaat agtcgggaga cgggtttatg gtgggcaaaa
    10441 cactcatata cctttgaaaa taaatacggc tggtgtgatc ccgccaattt ttgcttcatc
    10501 aataatcgca tttccagcca cgatagcaag tttcatacag gtgccgtggg ttcaaagtat
    10561 cggtgcgcaa ttagccccgg gttctttgtt gtatacgatg ctatacgttg gaatgattat
    10621 ctttttctgt tttttctata ccgctgtggt tcttaaccct gttgatatgg cagacaacat
    10681 gaaaaaatat gggggattta tccctggggt aagacctgga caaaaaactg cggactttat
    10741 ttataaggta ctgacaagga ttacttttgc tggagcaatt tatttagcaa ttgtatgcgt
    10801 tattccggaa cttttgattt acagactaaa tatgccattc tactttgggg gtacctcact
    10861 tttgattgtc attggagtgg gtttggatac cgcccaacaa attgaaaatc atatgctcat
    10921 gcggaattat gagggctttc ttggaaaagg ttccttgaag ggaagaagtg gttaacggaa
    10981 tgagggtgat ctttcttggt cctccaggag ttggaaaagg tacccaagcc gaatttattg
    11041 caaaaaaatt cgggattcaa aaactttcga ccggagattt gcttagggaa agcgttgatc
    11101 gtggtacaac gcttggaaaa gaagctaagc atttcatgga acgtggggaa ttagttcccg
    11161 ataatgtagt aattggtttg gttgaagaaa aattggcatc ttcagagtgc cgtgatggtt
    11221 ttctgttaga tggatttcct cgaacggtag cccaagcaga taaattgtca acgtttttgg
    11281 gagggaatgg agaggccatt gatcgtgttg tatatttttc tcttccagaa tctgacataa
    11341 tcgaacgaat aagtggaaga aggagttgcc caaaatgtaa ggcagtatat cacctcaagt
    11401 ccataccacc aaaaaaaaat ggggtatgca atgaatgtga agtgccactt atccaacgaa
    11461 atgatgataa accggaaact attcaatcca gattggttgt atatcaaaag caaacagagc
    11521 ccttaatcca atattataag gaccggggaa ttctttcaga attggatggg tcaggggtag
    11581 tttctgcggt tcaggataga ttggtagctc tgcttaccca gtcaagatag aatgatcatc
    11641 ataaaaacgg ctgaggaaat tgaactcatt gctcgtgccg ggaaaattgt aactcattgt
    11701 catcaattgc tggtaaatgc agtgagacca ggtataacta ccttggaatt agataggctt
    11761 acagaggaat gtattcttga aatgggggga attccagctt ttaaagggta tagaaatttc
    11821 cccaatagtc tttgtgcatc tattaacgaa gaagtcgttc atggaatacc ctcaaggcgt
    11881 gaattgaaag agggagatat catcggattg gatgtggggg ccattgttga ggggttttat
    11941 ggagatggag ctgtgacagt ttcggttggg gccgttcctg aaaaaattca attattgatt
    12001 ggagtcactc gagaagccct acttaaagga ctcgaaaagg ctgttgtggg taatcgtctt
    12061 tcagatattt cttttgccat tcaaacacat gtagaaaatc atgggtattc ggttgtgcgt
    12121 gattttgtgg gccatggtat tgggcgccag ttgcacgaag agcctcaggt tccaaattat
    12181 ggaagaccag gacagggacc gcgtttaaag cctgggatgg ctcttgccat agagccaatg
    12241 gttaatttgg gaggatcggc ggtcaaagtc ttgaaagatg gatggacggc agtcacttgc
    12301 gataattcac tttctgccca ctttgaacac acaatcacaa ttgaagcgaa tggtgcgcct
    12361 cgcattttaa caggttagtc ctaggttgga ggtcaggtaa atttcctcat gggaaaagaa
    12421 gatattatcg aggttcaagg ggcagtaaca gaaactttgc cgaatgcgat gtttcgggtt
    12481 cagttagaaa atggacatag gatattagct catatttctg gaaagatgcg gatgcatttt
    12541 attagaattt tgccaggaga taaggtgaca gtggaattat ctccatatga cctgactcgt
    12601 ggacggatta cctatcggtt taaatagagg ttgttatcgt gaaagttaaa tcatcagtaa
    12661 aaccaatttg tgttaaatgt aaggtcattc gccgcaaagg ggttgtaaga gttctatgca
    12721 caaaccccaa gcacaagcag cgacaggggt aaggttcgtc aatgtttttg gtggatagat
    12781 taagccaatc cctagagaga ggaaagtaag aaatggctcg tatcgcaggc gtagaattgc
    12841 cggtaggaaa acggatcgat gttggattaa cctatatttt cggaattggt cctgccagtt
    12901 cacgggatat attgcaaaag acgggtgttt ctcctgaaac gagggtgaag gacctagaag
    12961 aagatgaagt ggtccgtttg cgggagacaa ttgacaagga ttacgacgta gagggtgacc
    13021 ttaggaaaga aatcacgatg agtattaaac gtttgatgga tatcggaact tatcgtgggt
    13081 tacgacatcg gaaaggtctg ccggtgagag gtcagaggac aaaaacaaat tcacgaacga
    13141 gaaaagggcg acgtggggct attgggggaa aagttaggaa atgagctgct gaaagggatc
    13201 tttcatgagt gttaaaaagg gaaaaaagaa ggatagaaaa gttttacaag tcggtatcgt
    13261 gcatatcatg gcctcattta ataataccat cgtgacgata acggatatga ccggtggggc
    13321 tgtaacttgg gccagtgccg gtagtcaagg atttaagggg tcgagaaaga gcaccccatt
    13381 tgccgctacg cgtgcaggtg aagtggcggc aaaaaaagca atggaatttg gccttcgaca
    13441 agtggatgtc tatgtaaatg gccctgggtc tggacgagaa gccgcagtga gagcccttca
    13501 atccgctggt ttgcgagtca atcttattcg tgatgtaacg ccgattcccc ataatgggtg
    13561 tcggcctcct aagcgaaggc gagtgtaatt tatcagagag gtttttgtag attatcggtt
    13621 tttcgtgaac tggagatagg gggagaaagt ccatggccaa atatactggt cctgtatgtc
    13681 ggttatgtcg gcgtgaggga gtgaagttgt ttttaaaagg cactaggtgt atgactgaaa
    13741 aatgcgcaat tgagcgtcga agttatcctc ctggacagca tgggcaatca cggcgaggcc
    13801 gtgcgacgga atatggcact cagctaaggg aaaaacaaaa gcttcggcgt gtatatggta
    13861 tgcaggaacg acaattcctg aatacctacc atcgagccgt cagacgtaag gggatcaccg
    13921 gcgagcattt gttgagcctg ctagagcgaa gacttgataa tgttgtgtat cgactgggat
    13981 ttgcctcatc acgtggccaa gccagacagt tggtgaatca cggtcatgtg atgttgaatg
    14041 gccgaaaagt cactattgct tcggtaaccg tgaacgtcgg ggatattatc gaaatcaggg
    14101 agaagagccg tcagcttgtt ccggtccaag ccgccctgga aattactagt ggtcggggag
    14161 ttccgaattg gttagagatg gagcggaatt tattaaaggg taccgtccaa gccattccca
    14221 ctaaacagga cattgatgtt ctggtcaatg aacagattgt agtggagctg tattcacggt
    14281 aaattttcct gccggagata gagcggatgt tgcgaatgga tcacatatat ggccttggga
    14341 ccaagaaagg gagacgcgat ccttatgata aatagcatga aggatttcca aatacccacg
    14401 aagttggaat tggaaaagga cactgcttca cctacttatg gcaagtttac ggccgaacca
    14461 tttgagcgag gttttggaac caccatgggg aattccctta ggcgggtctt gctttcttcg
    14521 cttcctggag cagctgtcac ctccgtaaag attgagggag tatatcatga gttttcgacc
    14581 attcccggtg tgaccgagga tgttacgatt atcattttga atattaaggc gttacggttg
    14641 cgtttgcatt ctgacaaggc taaggcgata cgcctgaaaa agaaggggcc tggggaagtg
    14701 ttagccgcag atttatctgt agacccggac gtggtagttt tgaacccaga ttttcatatt
    14761 gctactttgg ataaagatgg gatgttggat attgaactca ttgtgaaacc tggacgtggg
    14821 tatgttcctg ctgaacggaa taaagaagaa gggttgccaa taggggtcat cccggtagac
    14881 tctgtttttt ctcccattaa acgggttaat ttcagtgtgg aaagtgctcg ggttggtcgg
    14941 atcacagatt atgataaact cattctggaa gtgtggacgg acggaagcat aactccgcag
    15001 gaagctgtca cctcagcggc aggaattctt cgcaatcatc ttgatatttg cctgccatct
    15061 gaagaaaggg gagagccagc tcaagaagaa ggtggagatg agagcaagat gttgattaac
    15121 caacatctat ttcgaagtgt aaatgaatta gaactgtcgg tccgcgctgc aaattgcctg
    15181 aaaaatgcta acattaaatc aataggtgat ttggttcata aaagcgaaaa tgaaatgttg
    15241 aagactaaaa actttgggaa aaaatcattg aatgaaatta aagaggtctt agcagaaatg
    15301 gggttgacct taggaaccaa agtgagtgaa gtgacagaat cccaaacctg aacttttttc
    15361 aaaggacgtg tattgtgcgt catagaaaac gcggtagaca attaggacga aacagtaaac
    15421 atcggttggc tttatttaga aatttggtca catctctcat ggaacatgag cgaattgaaa
    15481 ccacggaggc taaagcgaaa gaacttcgtg ggatcaccga tagactgatt actcttggga
    15541 agcaaggaac tcttcaagcc agaagagggg ctttgcgtgt gttacgaacc aaacaaacgg
    15601 ttcaaaagct ttttgatgac gttgccaagc gttttcctga gaggaacggt ggctatacaa
    15661 ggattattaa aactcgtcaa cgcccagggg acgcagcaaa actggtagct attgaattgg
    15721 ttgaaaaaac gaatgaatcc gataaggaga tagtttcctc aacccccagt tagaaatgta
    15781 tagagcgtag tcttttaaat ctatcattct cactgctcct gttcctccag catagtttct
    15841 tctctcctcc ttcaatatgt cacagggtta aggtctttta tttacttgac ctggaccctg
    15901 tgctattctt ttttatgggt tttttaccct aagggtggtc gttcgaccga gcagggataa
    15961 atgcaatttt atggtacgag agccgtttta actctgctag ttttaggtat gacaatattt
    16021 atcggaagtc gtgtgataaa tcacatgcaa acacgaagcc aagacacaac tttggtaaca
    16081 ttacaggaac agcaaggagc tgacgcgtgg attcagggat ttaattaccg ccaaactcga
    16141 tccggctcca caaaatgggt tgtaaatgct gatcaagcca aggtctttga taaagagcat
    16201 gtagccaagt tggaaacggt tcaagtccac ctttttgatg gagagtttca aaaggaacaa
    16261 ttaaaaatta cctctgaaaa aggcgtaatg aatacatcaa gcaatgactt tgagttaata
    16321 agcaaaaatg aaaaaactgt tatgactttt gagtctggtt atcaagtgtt ttcagataaa
    16381 ttgacctgga acgaagaagc acggcagata cacactccgg atcaagtgct tatcaaaggg
    16441 gatggcttaa ccatcaccgg aacaggatta gaaggagacg tggataagca tgaatttcat
    16501 ttgctcaaaa atgttcgtgc tgagttggtg tcgccataag agtttcaatg ccgatctcat
    16561 tcggttggtt gatttttgcg attatactgg tccacgttcc cattgtggca atgggagaag
    16621 agccaaaagc ctctacggtc attacttcta agacgatgac ggccagtagc gaaaaaaacg
    16681 tggccatctt tcaaggagca gtcaaaatgg ttcaaggcga actggtggtg cattccgatg
    16741 tgatgatcgt gtatttcaaa gaaaaagcac cttcaaatcc ctctgccgca acagattcag
    16801 gagccaaatc agaatctgga aatgaaatta attatattga ggcgaaaggg aatgtgagaa
    16861 ttgaaaaagg ggaaagtcga gctaccagtc aacatgcctt gtattacaaa tcggaagaaa
    16921 aggttatctt gaccggttct cccgtagcct ggcagaccgg aacaagaatt agcggtccca
    16981 aaatgaccat gtttttaaaa gaaaaccgga gtatggtaga aggtggaacc cgggtaataa
    17041 ttgatggatc ggagcaaaat tagaatttgc aggcttgtga tccgactgcc atgattcttg
    17101 gtgtattcat cgtgctccca taaaactgga tttttgattg tgattgacaa gggccgatca
    17161 acccaatcat ggtttgatcg tagattctgt ggcctgagtt tgaggaatct gctcggttca
    17221 acgttttctt ggaaatcgag atccgcatga ttgaatcaag gaatgtcaat gcctgaagca
    17281 cgtgggaaat ctcttcatag aatttctgcc aaggatttaa agaagagcta taaaggtcga
    17341 caagtcgtta ggggcgtcag tttggaattg ttgggaggag aagttgttgg gcttctcggt
    17401 cctaatggtg ctggcaagac gacgattttt gatatggtgg tggggctttg tcaacctgac
    17461 caagggaaaa tatttctcaa caacgatgag attactttcc tgcccatgta caagcgggca
    17521 aggcggggaa ttggatattt gccacaagaa gcttcaattt ttcgaagact ctcggttgaa
    17581 gataatgtat tggcggtctt ggagacgtta aatctccctg cgtctggccg gaaagcgaga
    17641 ttagatgaat tgttagagga gttggatctg actcggcttc gtaggcatgg ttcctatact
    17701 ctctcaggtg gagagcgtcg acgtttggag attacgcgcg cattagccac aaacccaaaa
    17761 ttcttactcc tggatgagcc ttttgccgga attgatccaa ttgcggtggg ggatattcaa
    17821 gatatattaa ccagtttaaa aaggaaaaat atcggcatct tgattacaga ccacaatgtc
    17881 caagatacgc tttccattac cgatcgagcc tatataataa gcgaaggaac gattttagaa
    17941 agtggtccac ctgatgtcat tgtgaatagt ccaaaagcca gggctgtcta cctgggcgaa
    18001 cgcttcaaaa tgatgcaata aagaggaagt gggtttctgc aatggatctt cggttagacc
    18061 ttcggttatc tcaaaagttg gtgatgactc cccaactgca acaagcaatt aagttgttgc
    18121 aactctcacg tttagaactt cagcaaacgc tggctcaaca cttagtagaa aatcccctct
    18181 tagaagattt ggccctggaa gcccaggatg aagaaccttc agtgggagaa gagtctgtgg
    18241 gacaggtgaa agagcctgac accgaaagtg agccttcagg tgaggtcgcg gaggttcaac
    18301 caaccatcaa agaaggcgag ccgttgtcct ccgaagagtg ggacaatatt ttggggaatg
    18361 attggcggcc tgcccagcgg gaagaggcct actcgggaga tgaagagttc ccttcatatg
    18421 aacaaactct caccaaaccg acttctcttg aggagcatct ggattggcaa cttcgtctct
    18481 catctcttgc aggggatgac cgagatattg gcagaatgat cattgggaat ttggatgaag
    18541 acgggtatat gcgcgtgcct attgccgaat tagctgaaga cgccaaatgc tctgttgaac
    18601 atgttgaggg ggtgctccgt cagattcagc agtttgatcc tccaggtgtg gcggctcggg
    18661 atttggcaga atgtttattg atccaactgg atcatctaca acatgatgag actgaactaa
    18721 cctctcattt gcaagcagcg gtgcccatgc atgtggtggc tgctatcctg aaagatcatt
    18781 tagtcgatct tcagaaaaaa cggtatcagt ctgtagccaa aacattgagc gtgactctgg
    18841 atgaagtcta tgaagctgtt cgggtgatag agagtctgga accgaagcca ggtcgcccct
    18901 atttttcgga tagtaactca attattattc cagatgtcta cgtcgtgaag tatgaagggg
    18961 agtgggtcgt gctgttaaat gatgatggcc tccctcgatt gagaatcagt ccgtattatc
    19021 ggcgcctcct ttcccattcc ggcgaagatg ctgggaatac gaaaaactat cttgaagaaa
    19081 aattgaaggg cgcacaatgg attattcgga gtattgatca gaggaacaaa acgattgtga
    19141 aagtcgtcaa aagcctggtg aagtttcaag aagctttttt ggaaaagggg attcagtatc
    19201 ttcggccatt agtcttgaag caggtggccg aagatgtggg tatgcatgag tcaacaatta
    19261 gccgggtgac gaccaataag tatttgcatt gcccccaggg aattcttgaa ctgaaattct
    19321 tttttaatgc agggattcag cgggctgaca accgtggaga ggatatgtcc tctgtgactg
    19381 ttcgtgagat gattcgggag atggtggcac aagaagatgc agccaatccg ttgaaagatc
    19441 aggagatcgt ctccaggctc cgtcataaaa atattttgat tgcccgtcga actgtagcga
    19501 aatatcgggc tgaattacat atttcttctg ctagccaacg aaaaagaatt cccacataat
    19561 tcttgacagc ggacttcagg ccccagacac catagtgagt gttgcagaaa gctgatctca
    19621 ttgagtctca ggtttccgtt agtctggttg aaatgagtcg gtccatgcac gatgattgtg
    19681 gaagcgaaac gatgccgagt gagtcacctc aagcttctct gaaaaacgat ccgttgccac
    19741 ttcaactgat gatggtgacg gggacgtcgg gatctggcaa aacccaagcc ttgaagtgct
    19801 tagaggattt gggctttttc tgtgtggata atcttccacc ggccttattt caaaaattca
    19861 ctgaattgtg tattcaacct gacaaagatg tgaggaaggt tgccctgggg attgatattc
    19921 gtgaacgggc tttttttgag gatctattga gcaatctcga agctttacaa agtgatggct
    19981 gtcacgttga attgcttttt ttagaggcac atgatgatat tttagtcagg cgtttttctg
    20041 aatctcggcg tccgcatcca ttactccccc atcgaccgat tattgagggc attcaactag
    20101 agcgtgaacg cttacaaagt cttcgggcta gagcccatcg cgtgctggat acgtcggagt
    20161 tttccgttca cgatctcaaa gcctggatca ttaaacatta tcgagagagg ggtgaagggc
    20221 aagtcctgaa acttagtctc atgagttttg gctataaatt cggagtgccc ttcgatgtgg
    20281 atatggtgtt tgatgtccgt tttcttcgca atcctcattt cgtgcctgac cttcagcctt
    20341 taacagggga gcatggcgat attcaacgtt ttgtcctaga cacgagggag gcgaaagtct
    20401 ttcttgacca tttaaaagaa ttgtttgctt ttttgttgcc aatgttcgaa agggaacaac
    20461 gaagttatgt taccgttgct ttcgggtgta cgggtgggag acatcgatcg gtggcgatgg
    20521 tcctgcgtat gaaagagatt ttgaatattt tgggctatga cgtcacgatt caccatcgag
    20581 aaatatcaaa agaggcgaat ccaggctaat ttcccttcgc ccttgagtag ccttttcctc
    20641 agaattagtt cccacattcc agaaatttct gcttttaaag cccattcttt ttgtcattgt
    20701 cgccgtcctt tctttaagcg acggtttttg acctctcatt cagcaagcct ggcataatag
    20761 agccattccg aagggaaggg gaaggggatt ggtaaaccag gcaaacgagg aggattgtct
    20821 ccaagtgttg ggaccaaccg aatctaggta aaaacacgac atcatatttg ggaatggcat
    20881 ttacagagat aatccagata caacctcaat tgaggtaaag ggacatttgg ccattattgt
    20941 tggactaaat aagatgaatg caatttggaa aggcatagca gaaagggtca gtggatattc
    21001 gtggaaaagc cagaatgtta caggcgtgga tgtggggtct cgattgttga aggcagtgac
    21061 cctaaaaccg agatatggca gtcctttctt acagggtgtt gtgattgaaa cccttacctc
    21121 tagatctaat ctcgatcccc aatcggattt tgagcaggac cgacatctcg tccaaattct
    21181 ccgaaaacag ttatcccctt ctcctcgtac tgtgggcctt acggtttcga gttctcacgt
    21241 gttgttgaaa agactgacgt ttccccatat gtcggaaaat gatcttcgag aacacctggc
    21301 tttggagctt gaccggtata ttcctcttga tgttcaaggt gccgtgtggg acgtgtatcg
    21361 gcacaacgtc acaggaccct tggacaatgg aaaggatcaa gctcttctgg ttgtggcaaa
    21421 aaaagaattt attgaggaac gaatgcgaca atttcaacag caagggatga gtgtcggttt
    21481 tgtcgatgtg gatgctttct cactcgtgaa tatggttgtc tataactatg gccaagaagg
    21541 aacgtggctg attgttcatc ttggaccaac cggcattctt tcggtggtca tggaagaagg
    21601 tcatcctata catattcacc aagtatccta cgaggccgat tggtatggag acctgcttga
    21661 cgggatgcaa tcggcttctg aaacttctgg taatggctat aagataggag cctcggaatc
    21721 tttacttctc gagcaatttt ttaaggagac catcgagcag gttattcaaa ctgccaagga
    21781 tgtttcggaa ataactgacg ggggagtcgt gaggggcgta ttcctgtcag gggggtattc
    21841 ggtcctggaa ggactccaat caagactcgc tgattcattg gctgtgtccg taactccggt
    21901 caatcccttc aaaaagatcg cggtccctca ggacattcaa caggattgtc gatttcagaa
    21961 agccttgcct ttatttggtg ttgccgttgg agcggcattg cgttggctcg accctcatga
    22021 ttgaaattaa tttagcacgc caaattcagc gtcaagtgag tcctcataag tcttcctggc
    22081 tgatttgtat gttggtaggt ctggtggttt tcatgggttt tgggggggcg agttggtggt
    22141 ggacgaaatc tttgcaagaa caggttgatg ccttgcttca agaaaaatca gtcaaaatgc
    22201 aggctttagt tcggataaag gaaagacttc aaaatgtgga aagtcgtaag gagcaaaaag
    22261 cgcttcttat gacttccgtt gggcggctca gtgaccagga gaaagaaaag gcttggccgg
    22321 tagctttgct ggatggggtc agtcgaaatg tcggagatct tgatatctgg ttggagcggg
    22381 tacaactgga atcacggatg gtggaattac acggacaatc gttagcactg gaagatattg
    22441 tggaatttat ggaggcgtta gagaatgatc ggatcatcat gagtttgcct gtagtggaaa
    22501 ttcttgagcg cccagaagga gaatcagtgg ccttttcctt tctgattcgt tttgtatggg
    22561 atcatcaggt aaccacatga tggcgcagtg gaaggcacta tcgatctggc atcgattcgg
    22621 ttttctggtg ggggttatgc ttgtctgtct gttgggtaac cacctctttg tgtggcagca
    22681 aatgagtcac tcgcttgagg agttacaaca agaagtcgct cacctagatc aggaacgcca
    22741 cgggctcctc cagaaaacag actccttgaa agccatcgaa agagaaatca acgtcttacg
    22801 tgaaattttg tccgctcgaa ttcaacaact tcctgaacat attgaatcaa aagtttttcg
    22861 aagggatatc atggaaattg cgaagcgaag gggtgtgacg gttcgggggt ggaagcccga
    22921 aggtctttgg acggaacttc agggtgctga agcttcgatt cggattgccg tgagagtgga
    22981 aggggaattc gagggaacag ttcaattttt ggatgacttg cgtcagcttg catgggtcga
    23041 aagtatttct tccattgtca tggccggaag gtcagaaaac gaaaattcat ctacccttat
    23101 cacgaatctg gtgatgcacg ggttaacccc cttaggtatt gaacatgttc agcaactgct
    23161 taaaacatga tcgagccaat caactcctgg cgggaatgcg agggcacggt gtcatgttca
    23221 tgtggggtac ggtctcaagg atggcggtat actggagatg gtgcgcgagc tactctgtct
    23281 gtgtttgggg tgtgttgatt tttgttgggt taatgggaga ggctgccggg aaaggagcct
    23341 ccccattgcc ttctcatcca agcaggttag caaaaaccag tgtagatcag gataatgcca
    23401 gacacgctgt cgtgaatcgt cgagaccctt tcaaacgcat accgaagccc ctgccaacgc
    23461 ccaaggtttc gacccaatca gatacagaac cgaaagatgc tcccctcgca gataatccag
    23521 tctggagact tcttggtgtg atgcatggcc aggatggcca ccaggcggtc attcagatat
    23581 ctccaactga gcgagttttg gtacaaccgg gggttgaact tgctcgatca ggatggacga
    23641 ttaagaccat cagtgagaag gaggtgctac tggagtacgt ctcttcagcc tcctcggtga
    23701 ggggggcttc cccgacaaga accttcattc tttccttccc caccattcaa aaatcaccat
    23761 gaatattttt gttgagaaag cctgaaaaaa ccttcataat ccatactctt gaagtctagg
    23821 ggatgattcg tcaactcatt tgcgcgcatc gaaaggggtt attaggcgtt caacaatacc
    23881 cgtcgcctaa gttcgcagct ccatacttga tacgtttggg aatggataag aggagacatg
    23941 ccaccggcat gagtgtgtaa tcctgaaaaa aggaatgaaa tgttgagata tttaaatgcg
    24001 ggggaatccc atgggagagg tctcatggcg gtggtggagg gagttccgtc cggtttgccg
    24061 gtcgtggccg atgaaattaa tgcagatttg atccgtcggc aaggcggata tggtcgtggt
    24121 ggccggatgc gcattgaaaa agaccggatt gaatttattt gtggggttcg aaaagggaaa
    24181 actttgggaa atcccttagg tctgttgatc tggaataaag actgggaaaa ctggaaagac
    24241 atcatggcgt cagaacctgg tccaccctct accgaacgcg tggtgactcg tccacgaccg
    24301 ggccacgcag atttggttgg agccatcaaa tatgggcatc gggatatccg caatgtgctg
    24361 gaaaaagcca gtgccagaga aacagccatc agagtggcta ttggtggggt ggcgaaggcg
    24421 gtgctcgccc agtttgatat gcgggtggtc agttatacta tggatattgg aggcgttgcg
    24481 gcccccagcc cgaatgaccc gttgatggca tatgaatatg cagaacaatc tgacgtacga
    24541 tgccatgatc cggagacggc aaaaaaaatg attgaacaga ttcgggcagc caaacataaa
    24601 ggggactcct tgggtggaat ttttgaagtc gtggtgacga acgttccgat tgggctaggg
    24661 acttatgctc aatgggatcg gcgactgagt gctcgcttgg catttgctgc gatgagtatt
    24721 caagctatga agggggtcga aattggaatg ggttttgagt cggcccgccg ttttggctct
    24781 gaagtgcacg acgatatcta ctttgataaa gccacaggac aatttatcag aaagtcgaac
    24841 aatgctggtg ggttggaagg cggcattacg aatgggcaac cgattgtcct gcgagtggcc
    24901 atgaaaccca ttgcgaccct ttacactccc aaagacagtg tggatattga aactaaagaa
    24961 tcgtttgagg ccaccgtgga gcgttcggat atttgtactg taccggctgc cggtgttgtc
    25021 ggagaagcgg tgattgcata tgaaattgcc aatgccttaa ttgaaaaatt tggtggagat
    25081 acgttggatg aaatgaaaaa aaattttgag gcttatcaag agtatgtccg cacgttttaa
    25141 gtgctttggc ctgagctcct atcgtggcag atttttttcg ttgcttgtaa tgagaccgtt
    25201 ttctggtgat ttccgctaga tgaatatctc tgaactctcg aaacacgttc cggtttcttt
    25261 aggagaccgg agctaccacg ttcttatcca accgcaaatt ctgcctcaga ttggccgtgt
    25321 gcttcaggat gttggattat ccggtcaagt tgccattgtg accaattcaa tcgtcaatga
    25381 gctctacggt cgagtcgttt tccggtcctt gaagcggtgc gggttttctc cattttttgt
    25441 tgtgattccc gatggagaaa aagctaaatc tatgtattgg ctgtcaaaaa tactggatga
    25501 actggtcagg caacgacttg agcgccagga agtggtgttg gcgttaggtg ggggggtcgt
    25561 gggagatgtg gctggatttg cggcctctgt ctatctgcgc ggtgttccct tcgtccaagt
    25621 cccgacgaca ttggtggctc aggtagattc gagcgtgggg ggaaaaacag gggttaacca
    25681 tccaatagga aaaaatttgg tcggcgcatt ttatcagcca cgtgtcgttg tcgttgatcc
    25741 gcaggcgttg caatctcttc cgaaacgtga atgggtcgca ggtttagcgg aggtgattaa
    25801 gtatggcatg attgccgatc cgaaattttt tgaatattta gagcaaaaca tagatggctt
    25861 acgagaacaa gcagaggatg tgatccctac cgtcattcgg cggtgttgtg aaattaaagc
    25921 cgaagttgtg ggcggagatg aacgggaatc ggggcgccgc cgaattttga attatggcca
    25981 taccgtaggc catgcactgg aaacctgggg acgctataaa acatggattc atggagaggc
    26041 tgtgggaatc ggcatggtgc aggaagccgc aatggcgcaa tttttaggga tgtgtactaa
    26101 tgagcttgtt gagcggcaac gtgatttgat tcaggatgtc gggttgccca ttgctatgcc
    26161 ctcaatgaca ttcatggatc tctggggggc catgcaacat gacaaaaagg tggtaaaggg
    26221 ccaaatcaat tgcgtggtac ccacctcaat tgggaatgtc caagttgtgc cccttgttcg
    26281 gcaacgtatt cgtcaatggt tcactgccag tcagtcctcc cgcgtgaaac gattgaagtc
    26341 cgttccagag acattgagtc gtccacgtct atccaggaaa caaaaataac atggctcagg
    26401 tccgtccttc gaccatagaa gacctgaagc acctcgttcg ggaacggagc cgtgaagtgc
    26461 agattcttca ccgaatcagc gaatccatta gtggttcttt ggagctggaa acagtattaa
    26521 agcaaatagt ggaggtggtt gttgaagtga cgaaggggga tgcctgtctg ttgtatttga
    26581 tctctgaaac aacagacgaa cttattctgc gtgcatcaaa aaatcctcat ccccggctca
    26641 tcgggcggat atctattggt ctgggtgaag ggattaccgg atgggtggca cgagaaaaag
    26701 ttccagtggt gatttcccgc aatgcaagtg atgacgccag atttaagttt tttcatcgtc
    26761 tgccggaaga tcgttttcag gcgtttgtgt cagttcccat cacaagtaag gggagaatga
    26821 tcggagtcat caatgttcag cataagcggc cgaaacggta tgccgaagaa acgctagccc
    26881 tcttactgac cattgctaat caagtcggcg gagccattgc caatgccaga ttgtatgatg
    26941 atatgcggca aaaagcgcgc cggcttgaaa ctctttccca agtgtcggag actgtggcgt
    27001 ctaatcgact gatagaggat gtgctgcaat taattgtgac tatgaccgct cagctgatga
    27061 attcaaagat ctgctcgatc atgctggtcg accaggcttc tggggaactt cgcattgctg
    27121 ccactcaaag tctgagcgat gcgtaccggc ggaagccacc cttgaaggtt gggcagagca
    27181 tgagtggtcg ggctgtgcag gatcaccatc cggtgtatgt ctcggatgta aaggcggaag
    27241 agggatattt ttatcgggat ttggcgaaac aggaaggatt gcattcttta ttgtccatcc
    27301 ccatgctcat gaaagaaaat ccgattgggg tgataaatgt atacacttcc agttaccatg
    27361 cgttttcaga tgaagaagtg atcacgcttc aagccattgc gaatcaatcc gccgtcgcca
    27421 tagagcacac tcggcttatg gaaaaatcgt ttgagatgca ggaggccctg gaagtgcgaa
    27481 aattggtaga gcgtgctaag ggatttttaa tggcgtctag gggattgtca gaacctgaag
    27541 cgtttcgtct tatgcaacga caaagcatga atcttcgaaa atccatgaga gaaattgcag
    27601 atgcgattat cctcgcggag gaacttcaac gggcgactca gtaggcatcc ctaggacgat
    27661 tcatcatgga cataggaatt tgaggaaagg ttttttgaac ggactcttca tcacttgtat
    27721 aatacgctga agggcgacaa cgacgtcttc ctgtatatgt tagctcaaag gacaatggcg
    27781 tcctcggttc cgctgggaca gaggacgttt tttttatcat gatgcttcga ttagcaatgg
    27841 tgcagatgaa tgcggtcgtt ggggatctag aggggaacac gcgcaccatt tgcggatgga
    27901 ttcgcgaggc caaaaaagcc aaagcggatg tggtggtctt tccggaattg gcggtgtgtg
    27961 gatatcctcc agaagatctt ctcctcatgc cccagtttct atttaatata agtcggatgc
    28021 gggaccgtat tgccaaggtc acgaagggga ttatggccgt agtgggaagc gtaatcgaat
    28081 ctgatcgtcc tgctcaacat tccatcctgg gctccgggct tgatcgaagg aagcccttaa
    28141 atgcggcgtg ggttttggtc gatggacagg tgaaaagatc atatgccaaa tcaaagttgc
    28201 ccaattatgg agtgtttgat gaagaacggt attttcaacc gggacgaacc agtccggtct
    28261 actgcctggg cccgttacgt atcggaatca atatttgtga ggatatatgg tttgccaatg
    28321 gtccggccag ccagcaggca gcgtatggcg gtgctcagct tatcctaaac atcaatgctt
    28381 ctccttatca cattgggaaa acccaaagtc gtgaacacat gttggcggtt cgggctaagg
    28441 ctaacaatgt aatggttagt tataccaata tggttggagg ccaggatgaa ttggtctttg
    28501 atgggaatag tctcgtcatg gattccaccg gtcgaatact tgcgcgggca aaagccttta
    28561 aagaagattt cttgcttacc gacctcaata ttccgtcaat acgaaagggg aaattcaaaa
    28621 ggtctggaaa agaacctgaa ttcgatgccg atgaggttcg gattccgatg atccgcatgg
    28681 gatggacacc aacgtggccg aagtctggag ttccaagtgt cataaggctc acaccggtaa
    28741 tcggagagat ggaagaaatc taccgggcac tcgtgctggg agtgcaagat tatgtgagga
    28801 agaatgcctt ctccagggtt cttgtcggta tcagcggagg gattgattcg gctttgaccg
    28861 cattgattgc ccgagatgct ttaggcgcgt ccaatgtcat gggtgtcatg atgccatccc
    28921 cctatacttc taaggaaagt cgtgaggatg ctcgccggct agggaaaaaa ttaggaatcg
    28981 atatgcggag tctttcaata tcaggcgtat tcaagacttt tcttcgggtg ttgggcaaat
    29041 cctttcacgg ctgtcaggct gatacgacag aagaaaatct ccaagcccgc atccgaggaa
    29101 ccctgctgat ggctctttcc aataagttcg gatacttagt gctgacgacg gggaataaaa
    29161 gcgaaatgag tgtggggtat gccacactgt atggggatat ggctggaggg tttgccgtta
    29221 tcaaagacat tcccaaaaca aaagtgtatc agctttctcg atggcgaagc gagtataggg
    29281 gctcttgttt tgatcgagtg attattccag aaagaattca ggtgcgcgcc ccttccgctg
    29341 agttgaggcc tgatcagaca gatcaggatg ccctccctcc ctatccggtg ttagatgcga
    29401 ttcttcaggc ctatgttgag gacaatgagt caatgaatag gattgtgaag atgggctatg
    29461 attctcatgt tgtccgaatg gtcatgggga tggtagatcg cagcgagtac aagcgtcgac
    29521 aagcgccagt gggcattaaa attacttcga gagcgttagg aaaagatcgg cggatgccga
    29581 ttacgaaccg atatgtttcg ggagattaac ctgaatgcct ggcatgatca gctttagtgg
    29641 agttaccttc tgtgagtgaa gaccttcctt tgacccctcc cggtttaacg gaacacaagg
    29701 actcctatct ggggcttcac cttctccagg aacagcggga ggttcttcgg caaagattaa
    29761 tcgaaggggc gatgggcagt gaaatcaccc aggctttttc agattttatg gatgccttac
    29821 tgatcgcgcg gtttcgtgag gtcattcaac aaggcaatgc cggtgtacgc gctggctggc
    29881 agcagtgttg tttagtggcc atgggggggt atgggcggcg ggagttagct ccatattccg
    29941 acattgatgt catgattctc actcagggca atcaaggaga agtcgctgag gcgctttcaa
    30001 ccggagtttt tcaccggctt tgggatcttg ggtttcaagt tgggcatagt gtgcggtccc
    30061 tcgcagaatg cctgacgatc gcagaggccg acctcgctgc ctgtacctca ctgatggaat
    30121 cacgatttct tattggcagt gcttcagtgt ttcaggaatt tcaacgaaag ttcgcacgtc
    30181 ggatcatcaa gaagcgagcg caacggtttg ttctcgataa aatccaagag cgggagagag
    30241 aatatgccaa atttggtgag acggtattta tgctcgagcc gaatattaaa aaaagtaagg
    30301 gggggctacg tgatctgcat ttgttgcaat ggattggcca ggctcgatat ggggcgggga
    30361 cgattcaaga actctcgaat cggggaataa ttgctattca ggattatcag gttctgcaag
    30421 aggcacagga atttctgtgg cgagttcggt gttttttaca ttttgaagcg ggacgggctc
    30481 aagatatcct cacgttcgac gatcaggttc ggctctctgc actttgggga tttgtggatc
    30541 atcctcattt gctagccgtt gagcaattta tgcaagtgta tttccgtcat acgaccggat
    30601 tatttgaccg tgggcggcgg tttcttgatc aagccaaaaa gggaacttgg cttgaccggg
    30661 tccggcgatg gtgcccttcc cccttaatcg aaggacattt ccgtattcta gaaaatcggt
    30721 tgacaattca gaccgataaa ttacttgagg tgatggaaag ccctatgttg cttcttaagt
    30781 tatttatggt ctctcatcgt caggctgtcc ccatcgattc gtgcattctc aatgaattgt
    30841 cccagcacat gggaacccta cccaatgaac agtttcatat caaatcggtg agtgggcttt
    30901 ttcgacacat tttgtcaacc cctggtcgga tagctcaaac tcttgaggcc atgcatcagg
    30961 ccggtctact ggaaaaactc gtgccggctt tctcccgagt acgtggcctc atgcagttca
    31021 atcaatatca taaatacacg gttgatgagc acagtctgct cgcagtgagg gaagcggagc
    31081 gcctccagaa tcatcaagga ataatagggg aagtctaccg gaacatccct gataaagaca
    31141 ttttgcattt ggcactgttg cttcatgatt taggcaaagg gcgtccaggg gatcatagtg
    31201 aagtcggcca ggagattgcc cacttaacgg ctgatcggtt ggatttgtct gagaaggaac
    31261 gacaggctct ggcattcctg gttcgccacc atttgcagat gtctcatacg gcttttcgtc
    31321 gagacctgaa cgatcctaaa acgattgtca cattcgctcg aatcgtgaaa accgtcggga
    31381 tccttcagaa gctcttcatt ctgacgattg cagatatatc agcggtggga cctgaggtga
    31441 tgacgaaatg gaaggagtcc ctgttaggtg aactgtattc cctgacctac gcagagctcg
    31501 caggggacac aggtccatct aattccggtg atgggagtat gaatttttcg ccagaagctc
    31561 gaaatgaact tatcggaatt ttaaagaatt atcgaggcga aggacgttat cctgaattcg
    31621 actggacgga acgggtgaat gccacacttc aacaacttcc cggtcggtat gttcaatcga
    31681 caccggttgg ccagatggct gcgtatcttt tagccattca atctctgcct gtaaagtcga
    31741 cccaagtgga ggcaagattc aatcaaacgt taggcgtatc ggagtacgtg ctgatcacgc
    31801 gggaacagac caagccggga attttcatgc aagtggcggg agtattagcc accatgggat
    31861 tgcaggtatt ggaagcgcaa attatgacac tcaaggatgg cacggtgtgg gatgtgtttt
    31921 cggtctacga ttctgattat gaagggccgc catctgtcgg tcgcctagag gaggtggcga
    31981 aggagattca acaggtcgtg gagggaaggc aatcagtgca tcaattggtt gaacgacgac
    32041 ggcgcatggc ctttggccgt ccctttccaa ctggccggca tcccacggaa gtccatattg
    32101 atcagggggt atccgatgct tatacggtga ttgacatctt tgccgatgac aagcaagggc
    32161 tactctttgt gatcgctaaa acgctggttc aattggatct ctcaattcat atggcgagaa
    32221 tcgggactag gttagatcag gtggctgatg tcttttatgt aaccaactcc catggagaaa
    32281 aaattctttc cgttgaaaac tgtgagcaaa ttcaacatac tcttcatgcc gctgttgatc
    32341 aatttctgga tgaataatgt aggtggttct tgcgtatcat gcaccatcta ccggcacggt
    32401 gagtgatgga agagggcatt tggaatagac tccggctttt cagctagaaa cctatttttg
    32461 gccattaacc attgctgcat cttcagaaaa tttaaattca cgggagaggg tttcggaaat
    32521 tcccttatta ttgtcagggt tggatattaa tgcttggcgg cactgctgga catcctcctc
    32581 acattatctg gtcgggcatc agatttagcc taaaattgag gggttcatcc aatgttaagg
    32641 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctggtcgg gcatcagatt
    32761 tagcctaaaa ttgaggggtt catccaatgt taaggaaggg tagaaggggg ttaaggaaac
    32821 cactcttggt gattagcgga acaaacaggt ctaattgggc atagagaagc cgtctcatcc
    32881 aacaggtcag gggcgtcaga gaacgtagct ttagtccaaa ccggttctac cctcccatag
    32941 cggtgggcga tatgggcaaa taacgggagg gggcgtgtgt agttattgcc ccccttctat
    33001 ggttaccagg gttctgccgt gacttaatga tgtggtgaac ttacggtata ggataaagca
    33061 tttttgtcct agatagcatt aaagtgtctg gtgaacgagg tgggcatgcc gatggctttt
    33121 gatttttttg gattccttca tggcggctag tgcctgggat gtgattgcag atccgcaatt
    33181 cgtacaacac caaaaggctc cggttggtgc aagtgtactg ttctggcatt gtgggcagcg
    33241 ttgaaatggc atggttggtt cctttcttaa ggctaattgt gtcaaaaagc agcatcaaag
    33301 taagtagggg acccaggagt gcggatgggt cccctactag cttggagagg ggaagagtcc
    33361 tctccggggt aggcgggcag ttcgatgagc ggagatatcc ggataaatga atgttgagga
    33421 cgtttaacga caccatgttt gggtgatttg gtcatttgtc catcctcatc cctggtcttc
    33481 cacgaggcta cgagagtatt cctgagcggc ctctgagcga ttggggagcg gttgaagatt
    33541 gaaaagcatt gaagaggtgt tggtttgggg agttgagaaa atcatccttc ccgtgctggt
    33601 ttgcatgaat cgggtaacca ccacctcaat ggcttgaccg accagagtgg caccgtggtc
    33661 aacgacgacc atcgtgccat catctagatg tgcaatcccc tgcccttgcc cttgcccttc
    33721 cttgtgaatg aaaactcgaa tcgtttcccc aggcaatacc aaaggccgta attggtaggt
    33781 taattcgttc acattgaggg aggatacacc ttgaagtaat gcgacttttg ccaggttcca
    33841 atcgttggtc acgatcttag cttcgcgatt tttggctatg gcgatgagtt gatggtcaac
    33901 tgcggatatg tcgggttccc agtcctcaat tatcaccacc tccatgtttg gcaactgttg
    33961 gagttgagat aagatatcca agccacgctt ccccttaatt cgtttagagg ggtgagcgga
    34021 gtcggctaat gtttggagtt catgaagaat acttttggga accaggaaag gaccttccaa
    34081 aaaccctgtg gtgcagagag gaagtattct gccatcgata atggcgctag aatccagcaa
    34141 tttttgaacg gtgttcgatg gggtaagtga agtgtgggga gaagaatttt ttggttgttc
    34201 agcgataggg gccggcacca gtgtgttgga gatgtgaata ccgacaatca acccgatgta
    34261 gggacagaga agatacagag ggagtccgat ccacggggtg agggaagaca tctggaggag
    34321 acctgatccc attcccataa ttgataaaaa accgattcct gccagggcga caataagtcc
    34381 tatgctacca ccaatcaagg tggaacggcg gcatgacatg agttgtcgtt ctatcaatag
    34441 gcctatgcag ccgattgcca agccggtggc tcccaacagc acgatgtctg gaagggcatc
    34501 ggtccattga gaaggagcat gtcctaggcc ggcatacatc cctaccatga gacacaaaat
    34561 gagaaacagc cattggtgaa cggaaagaaa gggcgttttc gaaacaacag gtactgtggg
    34621 ggttggtgca atcatcatag agtatatcct tcaaaggtta ctgcaaattc gataagtgag
    34681 ggaggagatt catcccgttg gagcctcaag tggtaaacaa tgcgccaggg aatggtcgaa
    34741 ggtgagcctg gacttgagaa caggttgagc agatctctgg cagaaatcac tttctgcaac
    34801 ctcaagcaca gtggtaatcc ctcggaggtt cagatcatgg aatagacgaa attgtaggta
    34861 gcaacgatgg tcaaaaaata atgtttcgca tcgatggggt ggtaaagagt tggtgaggtg
    34921 cagggaacgt tccaagcgcc tgagaggatc agtcaataac ggaagaccaa agtcccgagg
    34981 aggacgacac caagaattcc caagtaaggc tttgtcagat attaaggccg cgagcacggc
    35041 cccacttgca tgaaaccggt cggcttgttg ttctaacaac caggcatcgg tttcagtaaa
    35101 ggcgggcaag cagcacaaca caaattggga tccctgaaat gctgttaagg gaagatactg
    35161 caatcctccc tttaacagac agggggtttt ccacattgga gcacgtgttc cgatcttcat
    35221 gttatttcct ccttggtatc cagtgaaaaa tatgccgggt ttgagaaatc gatccatgaa
    35281 tatgttgtgt ctgagcactt acgggcatcc tgggtagagt caggtggtca cttttattta
    35341 tatgaatttg agatccatta tcaatatcaa attatggtac ataaaaccac caaggattgt
    35401 caatttcaag gatactcatt tggaaatgaa tcatttaatg tggaaaacag gctggtgaaa
    35461 acaagtgatc agtgggaaaa gacggctgcc gggaggttac atcttattta tttgaattga
    35521 atgaaagaat aattggatga atataaggaa tggtgccact ggatggacgc tgctctctgg
    35581 gttgattttt aaatgggatg tcccctggca gtcaggtttt tcaatcaagt tgtagtagct
    35641 gcgttctgct tctcgtcagt cattccggtc tccccgtgtt tttcatctcg ttatttgatc
    35701 aatttagagg agggcaaagg tttgtcggtc tttttcctcc ttattcctcc gattccacga
    35761 tttggagatt caaaaagctg atttccttga gagagctttt cctgtatttc ctgaaatggg
    35821 atatggtgct gtggcctcct atgtgtgggt ttaattatcc tggcggtttg caagtatctg
    35881 atcatccacg taatcccccc aattaatgca ccgttatggg atgaccgcgg tgcctggaat
    35941 ggctgattca ctcattcctc tctacccaca ttcgcgtcca ttaaacattc actccatctt
    36001 gaaaattgtg tgaaactgaa gttgaaaatg agcatttatt aaatgctcac caaatggttt
    36061 tatgaaagaa tctggcgaaa ggtattgatg aggaaggggc ggtagaacgc cgcagggttc
    36121 ttcctgcctt atggtttact cgtgggctgg tgcggaagtt attttgtttg gttagttcgt
    36181 tcggtaaaga aggacattga tgtatgtgaa gaaagagagc gaactttttg caacgtcacg
    36241 acgacccagg gcaatcctcg gaaaagaaat gggtcatggc ttttgatgtt gcctggggac
    36301 ggcaatgggg catgttacat tgtcaggttg gtgggctgtc tggttgaggt gttggtattc
    36361 tttcacgtca gtgggcctca cattttgagc catccaccta cctgtttgtt tgggatcatc
    36421 cccaaccaac acgggagtct gctgggcaaa gtagacgtac cgatcctggg catactccac
    36481 catgaagacc cattggtcat ccatacaggc ttgatgttca atttcttcga tcgcttgggc
    36541 cgaccatccg gaccacgtaa tggggtgtaa agtgaaatgg tccgggatgc catctcccgt
    36601 tcggtcatag gccatggaaa tcaggccatt ggggaggggc tcccaggcta tgggatgatc
    36661 cggagttcct gattgaacac tgtgaacgat ttgggcctgg ccaccccaat agatcagaat
    36721 actcacacag acctgcagca aacgttttcc cattcttcaa tcctttccta atggcctatg
    36781 gccatcatga attcgggcca tgcccacaga ccaatgatac tgagaaaaac gagtcctagt
    36841 gatatcaaca agctgatgaa cacgggtttt tcgccttcca cgataaaaga atgcccgttc
    36901 atgtgagatc ctcctggaaa tggttttctt ggtacagggt gagagggata gcgatggttt
    36961 ctatttaaat gaaactcact atcaatatca ttattgtaac tctcttctcg ctcctattac
    37021 catgaccaat gtttagaaaa tgcatgttta agaaaggcat ttccttaacc gcgtgctcag
    37081 tcatcttcat gaaaatagat ttatcgatga aaggtactta ggcagattaa tttacttaag
    37141 agaaagggaa ctcaggccaa catcaatttc tgatgttccg taagggaaaa tttggtcaaa
    37201 ggaaataaga atcttagtta atgaaggcgg gctgggttaa agggcgtgga ctgaggttga
    37261 gtttttgttt catcgaagtt gattttgaat cttattaata agtatttgtt cctagatttt
    37321 tatattgacg ttgatggaag aaccgacgga taatgtctca ctctggcggc tatcacatgg
    37381 agtggcagtg agccctttga ggccccgctt ttttaattca tttctattta atcaccaaag
    37441 ggaggtgtgt aatgaaagcc aaaaaggggg ttgtggaatt tttgaatcaa attttaaccg
    37501 aggaattgac ggttattaac caatattttc tccatgccaa aatgtgtgat aactggggat
    37561 ataaccggtt ggagcataaa gtgaaagaac ggagttttag cgagatgaaa gacgccgaca
    37621 aaattattga acatattctg tatcttgaag gcgtccccaa cgtccagcga ttgggaacgg
    37681 tgaaaatcgg cgagtccgtg cccgaacaac tgaagtcgga tttagcgaaa gaaaaaaaga
    37741 tggtcacgct cctgactgag gccatccaac attgtacaaa ggttggagac ttcacgacgc
    37801 ggcatttgct cgaagatatg gtggcagatg aagacgaaca tatcgattgg attgaaactc
    37861 agcaggaaac aattaagcag attggcctgg agaattattt gagcgagcaa atcaagaatg
    37921 aggggtaaga cgtcaattgt ttggcgtcag gagaaagagg agggtctttt ttcgtccgtc
    37981 cgcgagcttt ccaatatatc tgcataaggc gaggtgtctg tatccaacag gcgcctcgcc
    38041 catcagggag atgaaccggc tttttgcttg tcacatacgt aaactgtcat tttagtttga
    38101 gagtgatgtc ccgttgcctc caaagaggaa cccttctctc tccctccgtt gttcgtggaa
    38161 atccgctgtt aagatttgtg tgagcctaga cctgttcttt tgtcacatga aacgaggata
    38221 catttttttt cgtaatgctt cgggacgtta cagaagagga cgtaacggct tgaatccagg
    38281 agaaccttat ttttcctact gttgccagga ggaagatcct ctatttcttt gattaactcc
    38341 gtggcagcat gttcgttccc aatgtggccg gtgacagttc ctttatgatc cttctccttc
    38401 atccgcgcaa ggttggccgg attcagggca gtacggagga tcaccaattc atgtggcatc
    38461 gtttcggatt tcactgctta aatggggatc cggcacgctt tggccatggt ttgagatgtt
    38521 cctttgattc gtcaactcag ttaagaggca tgacggatgt tttccatgat catacttgat
    38581 ggatgacgaa catttttttc aatggcaatt tgagattgct gcgtcaacaa gatcgggtgc
    38641 ccttcgtaac ccagcggtgt ttgatacctg agctgccatt ctcgtcattt gcatggcata
    38701 ggcgaggaac tcttctttct caaaatgaag ggtgactgac ttataggtga gatgaattcg
    38761 actacaatcg cagacattca gtttgagttg ttgacttggt ggcataggtg tctcctgcgt
    38821 ggtatgaggt tgatttgtta gtgagtgtca gcagaggctt ggattcaggc cctgttcagc
    38881 aaatatgaaa ttgtcggtgg gaggaaagtc gatatgttct tgagtggcct gaggataaaa
    38941 aacttggcat tgcttcaata tgcccaaaag gagggtcatg caatgccagt tgaacgaatg
    39001 agtcagtttg tttctgacta ctccctctgg atcgaagatg aagctctggc attggtttaa
    39061 gtgagtttca ctcgataagc ccaaggctcg acctatgcgt tgtgcggagt cagtcaataa
    39121 agggatggaa agaattttgg ctttggggag gtgggcagcg agaaacgggt gagctgggtg
    39181 ttggataccc aaaagggcgg cgccctgttt ttgaacggct ctgcgaaact gattgagaaa
    39241 cacggcatcg caaaattcta ggcggggcag gcaacatagc atgccccatt gtccctccag
    39301 actggctagg tccacatcga tcatattccc cttcagtagt gcaggaactc gcatacgtgg
    39361 aatagtggaa tgtcctatgg tcatggcaat ggcctccatt gtgtttgtgt gtgtgtcctc
    39421 caaccaaaaa aaggggttgg gcgcccggga ggagggaacg acgcccaaca tttttgagca
    39481 ggtactgggt agctcgccct ggaatatagt ttgtattgat ggcttttagc gttcattggc
    39541 attttgtgtg ggctggactt cagatacatc ctcaaacaga gcccggaaac atgagggaga
    39601 gtaacctacc gcatttttga aaaaatgact aaaggcaaac tggtcgctaa acccaagggc
    39661 tgtcgcaatt tccctgaggt tggcatgacc ttcttgtaac agaaatttag ctcgttctat
    39721 tcgcagtctt ttgagatgga aacggaatga ttcaccgata accgtgtgaa agagttgcga
    39781 acagtacttt tccgaatacc ccaagaagag ggaaatgtct ttgagggtga gcccctgatg
    39841 cagatgtgag atcatgaagg tgtgaatctg ttgagcagtt gaatgtgaaa atgcccgggt
    39901 atcctttaac ccggagagga ggtcttgaag gattttgcaa ataaatgaag atcggtcttc
    39961 gactggattt gtcccgattt tcacgtgagt cccttctcgc gccaaacagc gtaattcctc
    40021 tatcagcttt tccaggtgat cttgtgaatt ttgttgttct cgttggcatg tcatggtctg
    40081 ggtgcctcga ccacattgga cttcagtgcc ttttattgta agtctggcaa taggcctccc
    40141 atcgtggcag gtccaatcag atggttgagg gttagttggt ttggatattg tcttcttcat
    40201 tatttcctat aacgtcactg gtctggaggg gaatcttaag agaatttata ttggaaatat
    40261 ccgtcgtctc tgtaattcgt catttaatga taatgaaaat catttatgtt ttcaataata
    40321 gataaaaata catgctttgc ccatgttcaa ataacagaaa aaccatgttg tttgtctata
    40381 actgaaattg agcgatttcc tgtcagccag aaggtttttc tctgtcgagg gcatccccag
    40441 tcagcctgag ccattggcct ggagatgcaa aatcctggaa ataataggtg cttgggccgt
    40501 gattaacgag cgtgagacgg gtcctccttt tgcgcccatg atttggatag acggtttgac
    40561 tggatggacg gggagtctat aaggctgatg acgttgttag aaagagagaa tcggcaagga
    40621 tcattgactg gtagtagtca caggcaacac ctcgacaaaa acaacttcct tgaaagttct
    40681 cgagagaaaa aatgggataa aaacaaccgg gaggatcaat cagtgaccgt aaccagatat
    40741 catcgcacat gcccgatgcg caccttcatc taatcgttgt acccgttgta ccacaaggaa
    40801 ttcctttccc ggatctctta gcgcaattcc ttccatgagc ctcacgccga gcctagggct
    40861 cgacttggcc atgctatttt tggggccata gacagccgag gaggacatat aacaattttc
    40921 tgtcggcaac atctcacgct tctggtcagg ggtgaagccc ctcagaatca tcttctcttc
    40981 catgccgttc cctccttctg agaacgactc actttgtacg gcatgttgtc tcactataaa
    41041 aatagaccac tcctaatgag gaaaatcggt ccgaatcgga atgggaggtg caaggaacgg
    41101 ggatgaacca tccttcgagg aaagttcctc ccgttgtatg gcccaaggtg atttctcaaa
    41161 tttgctgctt tcggagtcgt tggagcgaaa gaaccgtctt gcaaggagag gcattcaaaa
    41221 aaacaagccc acggtgaaag attcaccgtg ggcttaagga agttcagtct cttccagttg
    41281 ttagccatac atgtgtaagg ttttgtctcg acgatatttg agtcgtttat tcgtagtctt
    41341 cgtttaagga tcgtaaaaaa gccgttaagg ccgggatatc ttctgggagt aagacgatat
    41401 ctttgagctg ggacgcgcca ttgcgtagtt tcccgtttcg cattaaatcc gaatttgtca
    41461 tataccctcg aattacatcc tccagggtgt cggcgatacc agtatgacta tagggagctg
    41521 aatgccctaa gtcgcggagg cctggagttt tgaaggctcc aatggctttt gggaggagtc
    41581 gatctggtcg acaggcctgg aaaccacgaa acactccata gtgttcattg agggtgtcct
    41641 cgcagaggat ggaccaaata ggtagctgag agccgggaaa gtcgggattc cagaaaatgt
    41701 tccaaatgcc caggtcggtc tgttgggggt tgtccggcga ggggatagcg cggaatggtt
    41761 cttgagcata aggatgcttt cccgtggcag gcaggtaggc ttcgatattt ccatttcgtt
    41821 catccaatct tggaatttga agcatggaaa acgattcggg accatgaatg cgttcatact
    41881 ctgtttgggc aattccagta ttgtgaaatc gaaaatcggt gaagttgggt ggggtatgac
    41941 atgccacaca attcccaatt cctcccttgg cctggtcact ggggcgaatc ttgtaacctt
    42001 tatgacgacg gtgtctgtgt cggtcctgtt gagcgaaaaa gattttcaac ccttttagtt
    42061 cctcaggccc aaaggtgaat ggttgattat ggaactgaaa cttgccatct gccgtatggg
    42121 gatttttctc cacgaattgc agggtcgggc tatcggaaga agagggtgag ggccatcggg
    42181 aataattttg ggaatcgtat gattcgagga attgaatctg ttggagcaac cgacgagtat
    42241 atgaatccgg agactcccat ttatccggtt ggcgtggcaa atcgttgatc tcaagaaagg
    42301 catcataggg agagagattg tagttccctt cgtcatcctg tgagaagagg agttgatccg
    42361 tataggcggc gatgagttgt gtgacggctt gaaccagttg caggtcggtg gcttgagaga
    42421 cctctacgcg gaaatcttca gggatcagaa attcttgagg aattgatgga tcggttccgg
    42481 tgaacaggac acgataggat agtccaccaa attcttgcgc aagatctccg gttccagagt
    42541 cttctcgtat aatcctggca atatgggaaa tggcggctgc ctcttcgttg ggaagccatc
    42601 catagtttct tccggtgagt gtgcctttga ccaaatccac catcgttggg aattcagcat
    42661 cggtatgaag aaaaaacatt tgtcgcggaa gagcggcatt gaccaatggg ggcgcgtttc
    42721 gaaccgatgt gtgtttttca tcttcacggg ctgggatagg acttcgtcgt gcaaaatccg
    42781 aataggtatt catgccgtat ttcggaatgt cgagtacttc atcgacaaaa tggcaacttc
    42841 ggcagttcat cgattggccg gcaaacgggc catcggcata ttgatctggc gggagttgcc
    42901 aattcgccac tttgtcgaga gcgggatcgc cttgcgtcaa gggatcattc acatcgcccc
    42961 cgtcatccag aaaattttta aataattgag caaatcgggt ttccagaaac aaccgttctc
    43021 cgttggtgac ttccggtgga tcggcttctt cggcttcctg cgctaacacc gcaggtgcat
    43081 tgacgagaaa ggatagggtt gatgtgagta aggaaataca aaagacctgc gcgcaagaga
    43141 aaataataac tctacggatg ttcatggggg cacctcgctt taaaatgaat cctttttcgg
    43201 agaaatcctc tttgatttct ctcttatgga tgaatggtga atgtatcctc acacgtcatg
    43261 tgtagaggag gatcaagacg agggggacag gaggaggcca aaccccctca ccctcatgag
    43321 gatgaatgca cgagtagtca tcctcggagt aaattaagaa tcagaacatg gcactctctg
    43381 accacaaaaa agcccatgag cgtagaatgg cagctcatgg gctctggact ccaaaagtct
    43441 cgggccttaa gaatgtggcg agacgtactt agtcgtcaga cattggcatc ctcaacgcgg
    43501 ccttttgtga ccgtatttca tttatgggta cgtcaaacat catgaaaaat ccaatgccga
    43561 attgaaagat ctccggccaa ctgtgatggc caacacaggg agatctgggt aaagagtcag
    43621 ggcacagatt ggaatttatg gcttttgagc caaaaaccag cccttgctgt tcatgcattg
    43681 tgccaaggca tcatcggtta gtcctttgct gaaggggtta aatgaggaat cggaatgagc
    43741 gtcattgatc aggatttccc gacatgagcg cagatcctgt tcgaattcac cctcgc
//
LOCUS       DAORAQ010000007        42907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_203_length_42907_cov_60.803813, whole genome shotgun sequence.
ACCESSION   DAORAQ010000007 DAORAQ010000000
VERSION     DAORAQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 42907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42907
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_42907_cov_60.803813"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..259)
                     /locus_tag="PKM72_01465"
     CDS             complement(137..259)
                     /locus_tag="PKM72_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59473.1"
                     /translation="MLSDTTALDRAELPERERISLADHMGVPLEEIPHQYGSVE"
     gene            complement(558..770)
                     /locus_tag="PKM72_01470"
     CDS             complement(558..770)
                     /locus_tag="PKM72_01470"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HNP59474.1"
                     /translation="MGVSAQRQSVLRCPQCGFQKTETMSLDSCRVMYECLKCRTILKP
                     RKGDCCVFCSYGTVSCPPQQTTHLDT"
     gene            950..1351
                     /locus_tag="PKM72_01475"
     CDS             950..1351
                     /locus_tag="PKM72_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNP59475.1"
                     /translation="MDTIGVLSRKTGCHIETIRYYERIGLLSKPPRTEGGHRLYDKEP
                     LRRLVFIRRSRELGFSLKEIRTLFKLVDGERYTCQEVKGVTEQHLRDVSKKISDLRRL
                     QKILRSISSQCEGGLVPDCPIIESLFEEKCP"
     gene            1348..1674
                     /locus_tag="PKM72_01480"
     CDS             1348..1674
                     /locus_tag="PKM72_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HNP59476.1"
                     /translation="MNTLAVYVGAALAEIGGCFAFWMWLRLYRTPWLGIVGIVSLVIF
                     AVILTRSEAVFAGRAYAAYGGVYIMASLVWLWAVEGMPPDRWDMVGAGMCLAGAAMIL
                     WAPRMG"
     gene            complement(1879..2556)
                     /locus_tag="PKM72_01485"
     CDS             complement(1879..2556)
                     /locus_tag="PKM72_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNP59477.1"
                     /translation="MKIKRMNFGYLLGGLLILMLSVALAQEEGVMGETRRLFLEPVLC
                     LMLLMGIWSHVSEKKWLMIGGGILAVSGVATATIDYFLGIPELHLVNMSILFVFSLVS
                     TWIAYRHLLLTGSITVNKIIGAICIYLLIGLNWAMFYLFTNLANPNSFQGLTSTAIGV
                     QFSELMYYSYITITTVGYGDLTPIKPIARTLTYLEAIVGQFYVAVLVAWLVGMYLSDK
                     GDPLKKS"
     gene            3242..3595
                     /locus_tag="PKM72_01490"
     CDS             3242..3595
                     /locus_tag="PKM72_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59478.1"
                     /translation="MNRGWYWVQRICAWVVTIILLSACSQNRAYLYETGERPIPPGIE
                     NQILPHKTTQDEVLTLLGEPIARLKTKTGGGHIHIWTYSYMDLQSPSHDKGESLTITF
                     DDKSFVVLSVTRGPL"
     gene            4029..4640
                     /locus_tag="PKM72_01495"
     CDS             4029..4640
                     /locus_tag="PKM72_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HNP59479.1"
                     /translation="MAAAHTLIACHECDLLHRMHPLYYGERAKCSRCGASLYAKKRDS
                     FEHTIILALASLIFFTIANVFPFMTFELNGRVQESLLSTGVMEFFEREMWALGVLVLC
                     ASILFPALKILGMLYVLGPLELNKRPWHAALVFRMVEHCRTWAMMDVYLLGVIVAVVK
                     LSDLANLVPGVAIYSFVALILTLAAADSALDTHAVWEKMEEIS"
     gene            4637..5272
                     /locus_tag="PKM72_01500"
     CDS             4637..5272
                     /locus_tag="PKM72_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HNP59480.1"
                     /translation="MMGLYPTAARSGLMSCHACHLLSPIPPLSSEGEAICPRCEAPLH
                     LRKPNSISRTWALLIAAYILYIPANLLPVMTVISFGKGEADTILSGVKELIHAGMMPI
                     ALLVFFASITVPVLKLLALTYLLLSVQYKSQWRPRERTMLYRMTEVVGRWSMIDIFMI
                     SILIALVKLDAVATIEPGAGAISFAAVVILTMFAAMSFDPRLIWDSIEEKT"
     gene            5269..6945
                     /locus_tag="PKM72_01505"
     CDS             5269..6945
                     /locus_tag="PKM72_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNP59481.1"
                     /translation="MKDSEPNPGNFEELAEVTVQKKRGISIVWIIPVVAALIGGWLTY
                     KTISEKGPTVTITFEDGAGLEAGKTKIKYKSLEIGTVKRVEISQDLSRVNVIAEFRKP
                     AEPHLTENSRFWVVRPRLGLGGISGLETLVSGAYIAMDPRPGPSALTFTGLEKPPGVT
                     REDKGAQFQLRAENLGSSAPGAPVLYHDIQVGRVLDYVLDEEGEGVFIDIFIQDPYHL
                     RVEDTSRFWQLSGFEISVGAEGLNVKMESLSSLLTGGIAFDTPAMAGGSGEPSQPGTA
                     FKLFRNFSSIQDEKYVLTRPFMINFDGSVRGLSVGAPVEFRGIKIGIVSDIAVNLDPN
                     TLDIKIPVLIEIQPERITTTQVLQSQIMQAQVSDDRYAVMKHMVKRGLRAQLETGSLL
                     TGQLFVGLDFHKDLPQKELNLTGKYPEIPAIPATMDELRRTVTDVMAEIRRLPLDKIA
                     KEILETVEGGNRLVNSPETQNAIHNLNAALGNVEKLTGGLDQQVDGLMMNLDKTLGTV
                     QKGLRIVDPDSPAAVNINNTLKELSAAARSIRVLADYLEQHPEALVKGKQ"
     gene            6956..7591
                     /locus_tag="PKM72_01510"
     CDS             6956..7591
                     /locus_tag="PKM72_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HNP59482.1"
                     /translation="MNRVIKGVFLFFACVGVLTLLGCGTSQPSHFYLLRAVSPASVSG
                     LSDTKPSSLSFGLGPITLPKYLDRPQIVTKTSAHEVELAEFHKWAEPLSENVSHVLAE
                     NLSALLSSDRILQYPWRSSIPLDYQIAVDVLQFDGTMGGEAVLVARWSLLGGDEQTVL
                     TTRKTQMTHAPTSQNYEALVEAMSRNLEDLSREIAEALTTLSPKIQSRSLP"
     gene            8030..8431
                     /locus_tag="PKM72_01515"
     CDS             8030..8431
                     /locus_tag="PKM72_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNP59483.1"
                     /translation="MWNRVWDADWREGGKGVVDVKGDEYAHNRGRLSIKGLMNREILY
                     GKVRALHPMIRKNGKLERATREEAMDLVAKKFRQAIDEFGPDSVAYYGSGQLYTQESY
                     IAKKLFKAGIGTLIRRLRRPLFTKANEGFGS"
     gene            complement(9392..10264)
                     /gene="atpG"
                     /locus_tag="PKM72_01520"
     CDS             complement(9392..10264)
                     /gene="atpG"
                     /locus_tag="PKM72_01520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNP59484.1"
                     /translation="MANLQALRRKIGSVKNTQKITKAMKMVAASKLRRAQQRILAFRP
                     YGQELRRVLANLSGRVNQSIHPLFQKRQVKIIQVTVVTSDRGLCGAFNMNVIRRALQM
                     IQKCEESGAKVIVGVVGRKGNEYFRRRHWPLRDPNVNVFDRLTYEHALEVGWAAVEAF
                     TEGAFDEGYIVYNEFKNVVQQQVVVDKVFPIEPLKEFGAAEAPSGGGYLYEPDQEDLL
                     STLLPKYAQGQVFRILLESSAAEQSARMAAMDGATRNAGELIKQLSLKYNRSRQEAIT
                     KELMDIVGGAEALK"
     gene            complement(10683..>11027)
                     /locus_tag="PKM72_01525"
     CDS             complement(10683..>11027)
                     /locus_tag="PKM72_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP59485.1"
                     /translation="FREVEDSLITIKKTGEQREAQESQVSSLQSALNFADKRYRGGFA
                     SYLDVLTAQRDLFQAELGLVSTRQQNLSSIVRLYKALGGGWSPTDKEGQPVSSPSEPG
                     PIDHEMQPELKS"
     assembly_gap    11028..11127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11128..12346)
                     /locus_tag="PKM72_01530"
     CDS             complement(<11128..12346)
                     /locus_tag="PKM72_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNP59486.1"
                     /translation="MNKCGAIVLSLLLFSGCTMGPDYKRPDVPTSDSWRLAPGTSESI
                     ANMPWWELFKDEELQKLIHTALQENLDLRVAAAAVQEFNNQLVIAKWDLAPSLGYEGK
                     ALYFHNTNNKGLETGSGLIPTPDGGNKKSGGLDYSREKFFGGIKWEIDLWGRIRRNVE
                     ATQAELLARVENQRGVVIALVSDVAQSYFTLRALDLQVKIAERTLKVWNEAVRLSRSQ
                     YEHGYASKLDLDRFEAEQAGTRAQLANLEQHVVLAENRISVLLGHRPMAIPRGFALTE
                     QPLPPEVPVGLPSSLLSQRPDILEAEQKLAAATANIGVAQAQRFPQFSINAGGGVAGF
                     QLNSMGTGPFAAANVSGTLTGPLLNATALGYGVGVATSQEQQALARYELAILNAFREV
                     EDSLITIKKTGEQR"
     gene            complement(12360..15587)
                     /locus_tag="PKM72_01535"
     CDS             complement(12360..15587)
                     /locus_tag="PKM72_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNP59487.1"
                     /translation="MAKFFIDRPIVAIVISIIMTILGLIAMVQLPIALFPDIAPPEIQ
                     LQATYVGADAVTVEQSVATPIEQQMAGVDNSIYMYSTNASNGQMTLRVDFDVNTVPND
                     DQILTQMRYLQAESQLPSDVKNYGVTIKKSTTSPLMLFSLYSPNGTYDEIFLANYAYV
                     NINDPLSRSQGVGQVTIFGAGQYAMRFWVEPDHLAKLGITVTEILDAIKKQNTVNPAG
                     QIGAQPVPQGQEFTYTVRSQGRLLNEEEFGQIIVRADPDGSIIRLKDVARVELGAQSY
                     SMIGRFNGKPSALIAVYQLPGSNAIDTVDRAKKLMEEMKERFPEDLDYAISLDTTLAV
                     REGIKEIVHTLFEAIVLVIIVVFLFLQGWRATLIPLLAVPVSLIGTFAIFPLMDFSIN
                     TLSLFGLVLAIGLVVDDAIVVVEAVEHHIEKGLSPKDATRKAMEEVSGPVIAIALILA
                     AVFVPTAFIPGITGRLYQQFALTIALSVIISAFNALTLSPALAGMILRPRTPARGPLG
                     AFFGWFNHWFGRATDGYVGYCHYLIRKSGRAMIFLVVAAVCAGLLGTKLPTGFLPMED
                     QGYVYLNVQLPLAASLQRTDEVAKKIEAILSEAPGIQFVSTIVGFSLLSTVNTTYNGF
                     FFVTLAPWDERTKPEEKLQAIFKNVNEKLAALPEANAFLFPPPAIPGVGTSGGVSFIL
                     EDRSGQDVAFLADNLNTFMEAARQRPEIASMNTTFIPDVPQVFAHVDRDKVLKQGVKL
                     EDVYKTLQTFMGGVMVNYFNRFGRVWQVYVQAEGEFRTKAENVGQFFVRNKSDEMVPL
                     STLVTMEKISGPEFTMRFNGYRSAQVFASANPAFTSGQVMQALEEVFAETMPREMGYD
                     FIGMSFQEKVASEGVPASVIFGFSLLFVFLILAAQYESWGLPFSVLICTPIAVVGAFG
                     ALWALGMENDVFTQIGLVMLIGLSAKNAILIVEFAKMEYEKGRSLVDAALTGARLRLR
                     PILMTSFAFIFGVIPLVLSSGSGAHGRILLGVTVIGGMIAASFIAIFLIPVSFYVVER
                     LVHRHDDHPDRDPDDHDFVDPEFMEDEDAVGVIPQPKNAPTPFKL"
     gene            complement(15602..16906)
                     /locus_tag="PKM72_01540"
     CDS             complement(15602..16906)
                     /locus_tag="PKM72_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP59488.1"
                     /translation="MKESQPFHFLPSIGSTSMTIHRFNAFFQAPRPFIVRAQPQTARR
                     DFRGVASLMVCLLLGITLGCDRKEAESTAAPPEVLVAEVIQKDVPIYGEWVGTTVGYV
                     DAQIRARIQGYLLSRHYTEGSAVRTNDLLFKIDPRPYQAALDQAKAELARAGAAYKKS
                     QLDVKRNTPLAAEGAISQKELDDALQASAVNLASVASARAKLENAQLNLNWTSVTSPI
                     NGIAGIATAQIGDLIVENTLMTTVSQLDPIKVNFPISEQEYLKLAERIEKAGGQENLN
                     PSHEKRLELVLADGSIYPHKGRAVLADRQVDVKTGTITIVSYFPNPGNILRPGLFAKI
                     RTPIETRTAGLLVQQRAVKELQGTFEVAVIGKENKVAIRKVTLGPRIGSLWLVDEGLH
                     PGEMVIVEGLLKVRDGMVVTPKATSDEPPPGAESSPDPVSAT"
     gene            17313..18944
                     /gene="pgi"
                     /locus_tag="PKM72_01545"
     CDS             17313..18944
                     /gene="pgi"
                     /locus_tag="PKM72_01545"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNP59489.1"
                     /translation="MTSLTQRPVWKSLEQHYQQAKKLHLRALFADDPGRGRRMTVEAE
                     GIYLDYSKNLITGDTLRLLHELAESVNLRGHIQAMFRGDKINITEDRAVLHAALRASR
                     NASILVEGRNVVPDVHAVLDQMGDFSTRVRSGEWKGYTGKRIRNIINIGIGGSDLGPV
                     MAYEALKHYSDRNLTVRFVSNIDGTDLAEATRDLDPAETLFVVCSKTFTTIETLTNAQ
                     SARDWCLNALHDKQAIAKHFVAVSTNRNEVQKFGIDPANMFEFWDWVGGRYSLDSAIG
                     LSLMIGVGPDHFRDMLAGFRAMDEHFQSAPFEKNLPVLLGLLGVWYVNFFHAETHAIL
                     PYDQYLWRLSAYCQQLDMESNGKRVTLDGTVVDYQTGPIIWGQPGTNGQHAYYQLIHQ
                     GTRLIPCDFIGFCRSLNPSGRHHDLLMANFFAQTEALAFGKTAEEVETEGVSQTLIPH
                     RTFPGNHPTNTIMAEELTPAMLGKLIALYEHKVFVQGIIWNLNSFDQWGVELGKVLAN
                     RIAPELTTPREPDLGHDSSTNALIQRYRQHRQIQA"
     gene            19220..19927
                     /locus_tag="PKM72_01550"
     CDS             19220..19927
                     /locus_tag="PKM72_01550"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNP59490.1"
                     /translation="MRQSVERTILILFLFVFFLQGCAATQQRRDVVESGFLTEGEHGM
                     LTEGGSNEALLRYVNPDVDWRSYDKIMLDSVSVWKDPETQDVSPEDLKKLTDFAYGQL
                     HDALSLDYTFVTQPGPGVIRATFAITEAEASNPVADVVTSIIPQTRILTGVKGLIVGG
                     KPGFVGTAGLEAKFTDATTGRILALAVDRRGGTKNLRGMTNKWNDVEKAYTYWASAVR
                     YRLCMFRGDANCVEPES"
     gene            20954..21229
                     /locus_tag="PKM72_01555"
     CDS             20954..21229
                     /locus_tag="PKM72_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59491.1"
                     /translation="MNVRLWVGSCFLLLSACGPTQQWVKPGATEADLRSTITLCDREN
                     RHFNQEGISHYGPEDETVRPRNYRRGGGDMMREQCLQSHGWTLEYVE"
     gene            complement(21936..22712)
                     /locus_tag="PKM72_01560"
     CDS             complement(21936..22712)
                     /locus_tag="PKM72_01560"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNP59492.1"
                     /translation="MKKHETSSTPHRTSHMDGEERHLPFQRNLRFAQWHVMVSILIIL
                     AGVAGCAQTEQAKEVELSGFLEDYSILQPGKEGEALLLYKNPQADFSIYRAVYVEPAI
                     VLISKQSTVPQEELHRLADDLRSKVIWKLKEDFLVVPKLVPGALQIELALTEAEPSDV
                     GMDIVSTIIPPAAMVSGAKKLATGTQAFVGRASVEAKLTDGNTGTLLMAAADRRVGGR
                     TLDGSIDSWDDAHQAFEYWADKLAQRLREWGRKPIPATTN"
     gene            complement(23213..25279)
                     /gene="tkt"
                     /locus_tag="PKM72_01565"
     CDS             complement(23213..25279)
                     /gene="tkt"
                     /locus_tag="PKM72_01565"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNP59493.1"
                     /translation="MMPDKQLDQLCINTIRTLSMDAVQAANSGHPGTPMALAPVAYCL
                     WNRFLRFDPDDPIWGNRDRFVLSIGHASMLLYSLLHLCGVKTVNAKYERLGHLSVTLD
                     DIKRFRQLESKCPGHPEYRWTSGVEMTTGPLGQGVATSVGMAIAERWMAAQFNREGFP
                     LFDYRVYALCGDGDMMEGISHEAASLAGHLRLSNLCWIYDNNNITIEGHTELATSDDV
                     ATRFIGYGWNVTRVGDANDLDILTRAFNTFLATPDRPTLIIVDSHIAYGAPNKQDTSA
                     AHGEPLGEEEIRLTKQRYGWPEDAKFLVPEEVPGHFREGIGKRGRSLRENWFTRFEAY
                     TQAYPDLADQLHKMQHRQLPEDWDKGLPSFPADAKGLATRDSSGKTLNSIAQHIPWLI
                     GGSADLGPSVKTRLTFDGAGDFLSGTYQGRNLHFGIREHVMGAVINGLALSKVRAFGS
                     TFLVFCDYARPTMRLSAIMEIPVIYIFTHDSIGVGEDGPTHQPVDQIASLRAIPGLII
                     IRPADANEVVEAWRVIMQFRHEPVALVLTRQTIPTLDRTHYASASGVAKGAYTLTNTM
                     DGEPDVILIGTGSEVSLCVEASERLKAERINARVVSMPSWEIFEYYCRKHPEYRETVL
                     PAQVTARVSVEQASTLGWERYVGSTGRIIGMETFGASAPLKELTKKFGFTVEHIVDAA
                     KDQLKK"
     gene            complement(25301..26110)
                     /gene="ppk2"
                     /locus_tag="PKM72_01570"
     CDS             complement(25301..26110)
                     /gene="ppk2"
                     /locus_tag="PKM72_01570"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNP59494.1"
                     /translation="MPKKQKEKDESKLKRKTYEKELRRLQAELCYLQDWVKEKGARIM
                     VVFEGRDGAGKGGTIRAITERVSPRVFRVVALPAPSDREKSQMYIQRYMGHFPASGEI
                     VIWDRSWYNRAGVEHVMGFCTKEQHERFLHLCPEVEKYIVDGGIILIKYWLEVSHEEQ
                     KRRFQARVEDPVRQWKLSPMDLPSLEKWYEYSRARDRMLDATDSKHAPWHIVRSDNKK
                     RARLNCISHLLSSIPYKRVKRDKVKLPARSKKYQYDDQAAMQKRSYIPENF"
     gene            complement(26242..28713)
                     /locus_tag="PKM72_01575"
     CDS             complement(26242..28713)
                     /locus_tag="PKM72_01575"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNP59495.1"
                     /translation="MKEELKEILAEYGCGPIQFSGTNDALYERHLIFDHVIDPKKADP
                     REQFEATARSVRDVISQRWLKTETTYEKKNPKRVYYLSMEFLLGRSLANNVTNALLGP
                     LALEAGKKRGLDPLAIAEIEPDAGLGNGGLGRLAACFIDSMATMQIPGMGYGLRYEYG
                     IFKQTIKDGWQCEQPDNWLRRPDPWEVPRLEESVEVKLNCSFELRDGILRMIPGRPTS
                     IIGIPFDRPVIGYGGKTINTLRLWAATVPDYFDFQRFSSGDFVAALAGTLAAESLTRV
                     LYPDDSTIRGQGLRFLQEYFLVACSLGDLVRRFRRGNTDWNALPEKVAIQLNDTHPTM
                     AVPELMRILLDEAQQDWDQAWDLTQRSLAYTNHTLLPEALEKWPHQWFELLLPRHLEI
                     IHEINRRHLDRARARFGGDESRLSNVSLIEEGAIRKVRMANLAIVGSHSTNGVAAIHS
                     GLLRSMTVKDLAEMYPERFNNKTNGVTPRRWLLMANPSLSHAITEAIGDGWITDLAQL
                     TKLKKLAGDKGFRDAARKAKRDAKSTFAKWLKSESGQTVDPDSIFDCQIKRIHEYKRQ
                     LLNALRIVILYNRLRENPKLQMAPRTFFFAGKAAPAYHLAKVIIKFLNNLAGTIDGDP
                     AVKDRLKVVFLPEYCVSLAERLIPATDVSNQISTAGYEASGTSNMKFMMNGGLTLGTR
                     DGATIEMAEEAGEENFFLFGLTADQVAGSRGWYNPHWHYEHEAETKRALDLIASDHFS
                     RYDPGVFAPLLHTLLAGGDHYMHLADLTSYLEADQRVCDLYADSDGWTRKAIMNIASS
                     GKFSSDRTIAEYATDIWNVKPCPVK"
     gene            complement(28831..30978)
                     /gene="glgX"
                     /locus_tag="PKM72_01580"
     CDS             complement(28831..30978)
                     /gene="glgX"
                     /locus_tag="PKM72_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNP59496.1"
                     /translation="MPKNQKRGAKQEVSMQAGTTAPGKSFPLGATVSPEGVNFCVFSK
                     NCTLMELLLFDHADDKKPVTIIPLDSWKNRTYHYWHVFLPGIRPGQIYGYRAHGPFEP
                     ERGLRFDSEKILLDPYGRAVAMPTQYSRVAASHPGDNCATAMKSVVVDMQAYDWEGDA
                     PLRQPSTRTIVYEMHVRGFTRHPSSGVAENTRGTYAGLIEKIPYLQALGITAVELLPV
                     FHFDPQDCPSGRVNYWGYAPVSFFAPHPMYSSRQDPTGVVDEFRDMVKALHRAGIEVI
                     LDVVYNHTAEGNEDGPTVCYRGLENGAYYILEPDRTCYSNYSGTGNTLNANQPIVRRL
                     IMDSLRYWVEEMHVDGFRFDLASILSRDEAGQPLQNPPILWDIESDPSLAGTKLIAEA
                     WDAAGLYQVGSFIGDSWKEWNGRFRDDIRDFFRGEEGSSARFADRLIGSPEIYGHKER
                     EPEQSVNFVTCHDGFTLNDLVSYNDKHNEANGEDNRDGANDNKSWNCGIEGPTDNEVV
                     ERLRNRQVKNFLAMTLLSVGIPMILMGDEVRRTQNGNNNTYCQDDETNWFDWTLVHRH
                     ADVHRFVTLLNARRLLRDVEREQQRMSLNQLIRQANKAWHGVKLNQPDWGPNSHSIAF
                     TVELPKEMLLVHFMLNAFWEPLEFELPTAHHDEDDIWYRWIDTALDSPHDIEAWESSP
                     AIPGRRYRTEARSVVVLFAHLNTSRTQTMSPGR"
     gene            complement(31103..31717)
                     /locus_tag="PKM72_01585"
     CDS             complement(31103..31717)
                     /locus_tag="PKM72_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNP59497.1"
                     /translation="MLKKLWNWFWSPSSKYSLGAIFVVGGIAGIIFWGGFNTAMEQSN
                     TLPFCANACHEMTDFVLPEYQQSIHYTNAQGVRAICADCHVPKQWGPKVIRKIKATWE
                     LWGKITGSINTREKFEAKREQMALKVWLEMKENDSQECRNCHSYEAMDWHAQSLVAQR
                     DMQKAMSEGKTCIDCHKGIGHTLPDGYESDTPVYKEDLAKLANK"
     gene            complement(31751..33283)
                     /locus_tag="PKM72_01590"
     CDS             complement(31751..33283)
                     /locus_tag="PKM72_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNP59498.1"
                     /translation="MKNIDDGSRLGNSLRISFCVILILLIGSSAMVLLDGATLLLRET
                     GEANAARPAGTYGTDWGNPDGEECVQCHRKHSPGLYQEWNQSMHGQRGVNCLDCHKAD
                     GKDKDGFRHKGQLISIIVTPKDCARCHTTEFQEMDGSHHSKAGDILASFDNLLGEVVG
                     GPEAVNAGCKQCHGSTIEIGEDGKPLPTTWPNTGIGRINPDGSRGSCSACHGRHRFSK
                     AQARTPDTCGKCHVGPDHPQIEVYNESKHGIIYHAKTTEMNLESDKWEAGVDYSAAPT
                     CATCHMSAGGGEKPTHNVGERISWTLRPPISVKNNLVKLKDGSEFDVPEGQPLPKVGD
                     KAKKSEVVEVLTWKERRLKMQNVCTACHTDSVILGHYNQFDKVVDLYNEKFAKPISAI
                     MGDLQKAGTISQAPFDEKIEWIWWEIWHHEGRRARHGASMSGPDYTWWHGMYEVAKHT
                     YFEFIPELKKVAGEKEAQALLEKHFKPIPGHAWYFEGMNPDQLDAVRKGFESRYGKGS
                     LK"
     gene            complement(33407..35815)
                     /locus_tag="PKM72_01595"
     CDS             complement(33407..35815)
                     /locus_tag="PKM72_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNP59499.1"
                     /translation="MANPLSKKELELIDAYWRAANYLSIGQIYLYDNPLLKKTLTKDH
                     IKPRLLGHWGTTPGLNFLYVHLNRLIKKHDLNMIYITGPGHGGPGLVANAYLEGTYSE
                     VYPNISQDEEGLKRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYAVSHAYGAAYDNP
                     DLIVACVVGDGEAETGPLATSWHSNKFLNPVTDGVVLPILHLNGYKIANPCVLARISH
                     EELDHLFRGYGYTPYFVEGDDPRKMHQLMANTLDIVTKEIQSIKADAQKKGATKRPRW
                     PMIVLRTPKGWTCPKEIDGKKTEGYWRAHQVPMGQMHEKPEHVRILEKWMKTYKPNEL
                     FDKNGQLNPELAELPPKGERRMSANPHANGGLLLKDLRMPDFRSYAVKVTSPGSVTAE
                     STRVMGTFLRDVMKQNLSSKNFRLFSPDESNSNRWQDVLEVTNRTWMADTFPYDDHLA
                     PDGRVMEMLSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIIDSMFNQHAKWLKVCDEIP
                     WRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVINKKADVVRVYLPPDANCLLSVT
                     DHCLRSRNYVNVVVAGKQPAPQWLTMDQAIKHCSAGLSIWEWASNDRGGEPDVVMACC
                     GDVPTLETLAAVDLLRTHVPELKVRVINVVNLMKLQPQIEHPHGLSDKDFDALFTKDK
                     PIIFAFHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTTTPFDICVMNDLDRFHLVDDV
                     IDRVPKLETRAAYAKQAIHDKLIEHKQYIAKYGDDMPEISGWQWGQRGTTGRRKSSTE
                     ADNV"
     gene            complement(35921..37174)
                     /locus_tag="PKM72_01600"
     CDS             complement(35921..37174)
                     /locus_tag="PKM72_01600"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNP59500.1"
                     /translation="MRVLVLNSGSSSVKFRVLELVPEGSTSGVRPARTPIRGAVKDIG
                     STASLELETDGTPHKKMTRLIADHHQAIRWLFEQLDQLDRGSKNAGSLTGVQAVGHRV
                     VHGGERFSESVMITDTVMGEIDALGELAPLHNPGCLEGIRGARDVLGPKVPMVAVFDT
                     AFHHTMPPHAKVYALPHKLADRHHIRRYGFHGIAHASLFENYIEKTGRPSGTSRLITL
                     QLGNGCSISAIKGGKSIETSMGFTPLEGLVMGTRSGDLDPSIVKYLCQKEKVDSSEVD
                     RWLNEQSGLLGLSGRTNDMRELLQAADREGHEPSQMAIDVFCYRAKKYIGSYLAVLGG
                     ADAIVFGGGIGEAAPTIRARICEGMEWCGLRLDSDLNHAAKGLQPGDVARISQNGHLL
                     EAYVGAADEETWIARETMRCLQATM"
     gene            complement(37188..39575)
                     /locus_tag="PKM72_01605"
     CDS             complement(37188..39575)
                     /locus_tag="PKM72_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HNP59501.1"
                     /translation="MIHHERVKPPPHIYPPDEWNLIEKQFHPPFLEMTESIFAIGNGY
                     FGMRGCGEEGVPAVQNGTFVNGFYESWPIVYGEEAYGFARTGQTIVNVTNSNKIKLYV
                     DDEPLYLPNANLLNYDRRLNMKSATLDREIVWETPSGKQVRIKSRRLVSFSHRHLGAI
                     SYEVTVLNAEAPIVLSSEMLYLHDHQKGNSDPRQAKGFLDRTLHPRKQIAKDQRIVLC
                     HGTENSNMTLACGMDHDLATDCAYTIQDKCYEDFGAIVFSIDAKPGIPVRLFKYMTYH
                     TTHTASPEGLCARAERTLDRALGQGFALILDDQEQFMSDFWSRSDIQVSNLDPTRTKR
                     SNIEIQQAIRFNLFHVLQASGRADTTGVPAKGLTGQAYEGQYFWDTEIYLLPFLIYTS
                     PRVAKNLLMFRYRMLDKARKWARELSQKGAMFPWRTINGEEASAYYAAGTAQYHINAD
                     IMYALKKYVQATGDEEFLNDYGAEMLIETARLWSDLGFFSDDKKGKFCIHGVTGPDEY
                     NTVVNNNTYTNLMARENLLYAASTIESLREKNPDAFAALTSKTGIEMEEVKDWRRAGE
                     NMHIPYNEEREIHPQDDAFLQKERWDLKNIPPENYPLLLFYHPLVIYRYQVIKQADII
                     LAMFLFGQAFSLEQKKRNFDYYDPLTTGDSSLSSCIQSIVASEIGYPDLATEYARAAL
                     LMDLADIGGNVKDGCHIASMGGTWMVLVYGMAGMRDFDGILTFRPRRLPAQPARIRFP
                     LTYRGQILDIDMGPEGAKYSLREGKGFMIRHEEEEINLSPETPVAIRPIVTWQSR"
     gene            complement(39693..40451)
                     /locus_tag="PKM72_01610"
     CDS             complement(39693..40451)
                     /locus_tag="PKM72_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HNP59502.1"
                     /translation="MSGAKSLYPITPDKFDAVLFDLDGVLTATAKVHAICWKKMFDEY
                     LQNRASRTGGPFQPFEIATDYRLYVDGKPRFDGVRDFLQSRGIELPEGTPADPPQTET
                     VCGLGNHKNEMINAVIETNGVEAYEGSIALVRRIRKDGIEVAVVTSSQNCDIVLNAAG
                     IGDLFKVKVDGNVIHQENLSGKPAPDAFLAAAKKLGVDPKRAVVVEDAISGVQAGRAG
                     GFGLVIGVARKQDSEELRKHGADMVVNDLGELLV"
     gene            complement(40587..41654)
                     /locus_tag="PKM72_01615"
     CDS             complement(40587..41654)
                     /locus_tag="PKM72_01615"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HNP59503.1"
                     /translation="MNHDSLQATAKALIAEGKGILAADETVGTITKRFESLKINSTEN
                     SRCAYREMLFTSPGVSEFISGVIMYDETIRQTNAQGVPLPEVCTRLGIIPGIKVDTGA
                     KDLAGSPGEKITEGLDGLRDRLTAYYNMGARFAKWRAVIQISDRHPSQTCLAVNAHAL
                     GRYAALCQEQGLVPIVEPEVLMDGPHSIERCGEITGHVLHTVFNALYAQGVSLEGMLL
                     KPNMVVSGKECTKQASVQKVAQATLQCLCRHVPAAVPGIVFLSGGQHDRLATAHLNAI
                     NILPSPKPWKISFSYGRALQDAALEAWHGDNNNIKAGQQALYQRAKCNGAASLGKYTE
                     EMEGNASRKRMPAHRADWHDD"
     gene            complement(41741..42187)
                     /locus_tag="PKM72_01620"
     CDS             complement(41741..42187)
                     /locus_tag="PKM72_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59504.1"
                     /translation="MLAPLIESKFTFIEVQVERSFFIFLETEQVCFDLPAEAFNPVHL
                     PIPLENTKDKNFTVRPSSKIPLDTLNPKVGVIALRQSAELQLRFTEWPETFSNHPHIP
                     IHPTENQSDRYENDKTVKSRGSIPYGLPGQLTRYSGAVLKPVFPSY"
     gene            42565..>42907
                     /locus_tag="PKM72_01625"
     CDS             42565..>42907
                     /locus_tag="PKM72_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNP59505.1"
                     /translation="MAENFQKVYVIRHGETEWSLSGQHTGTTDLPLTENGRNLARLLQ
                     PLFSNYSFDLVLTSPLQRAHETCVLAGLGEKAEVDTNLVEWNYGEYEGVTSKEIQEKR
                     PGWLIFTDGCPG"
BASE COUNT         9727 a        10935 c        11372 g        10773 t
ORIGIN      
        1 tacgctctgc gcgcaaaaat gactgcggcc ttgctggacg aacccttcga aagcctcagg
       61 acatgctttt ttgaaccgcc ccgaggcctc tgatatgcaa cgtgcctgtg ggtcaatagg
      121 tccttggaaa ttattattat tcaacactcc catactgatg gggaatctct tccaacggca
      181 cgcccatgtg gtcagccaga ctgatccgct cgcgttccgg tagctcggcc cgatcgaggg
      241 ctgttgtgtc tgaaagcacc aaacgccgga accatctaaa cccctttgtg acggagataa
      301 ggttctggca tccgctagaa tggtgacctg atgcccggca ttcactattg cccaggccac
      361 gttggggacc gggcaaatcc gggcatcatc ttcggccaag gaagacttca tatggatcag
      421 gatgtgttga ggctgaattt gctcagcctc aacggcccta tttccgtaag aagtggtaag
      481 acgacgacgc acaggcccaa aatgaatttc atgtttttct tacaccatct ccttcgcgat
      541 tgttacaact atggggttta cgtgtccaag tgggttgtct gctgaggcgg acaggacacc
      601 gttccatagg aacaaaagac gcaacaatct cctttcctgg gtttgagaat cgtccggcac
      661 ttcaggcact catacatcac ccggcaggaa tcaagtgaca tggtttcggt cttctgaaaa
      721 ccgcattgcg gacagcgtag gacggattgt cgttgtgcgc ttactcccat tggccattct
      781 ccattttttt ctgcatgacc gttcgtacta ccccaatctt cattccatgg agttagtcca
      841 agtcaggaaa cgtttcatac ttataagaca agtttcctcc ttcctgattc tcatggtaca
      901 acctgtagca actacagatg caagaaggtg tcttaaggag cgaggcagca tggataccat
      961 cggagtcttg tctcgaaaga ccgggtgcca catcgagacc atccgctatt acgagcgtat
     1021 tggtttgctg tcgaaaccac cgagaaccga aggcggacac cgtctctatg acaaggaacc
     1081 actcagacgg ttggtcttta ttcgtcgtag ccgggaactg ggattttcgc ttaaggagat
     1141 tcgcacgctg ttcaagttgg ttgacggcga gcggtacacg tgccaggaag tgaagggggt
     1201 aacggagcaa catctcaggg atgtcagtaa aaaaatctca gatttgcgta ggcttcaaaa
     1261 aatacttcgt tcgatttcct ctcaatgcga aggaggtttg gttcctgact gtcccattat
     1321 tgaatcgctg tttgaggaga aatgcccatg aatacgttag cggtttatgt cggagccgct
     1381 ttggctgaaa tcggcggatg ttttgcgttc tggatgtggc ttaggctata ccgaacaccc
     1441 tggttgggaa tagtgggaat cgtttccctt gtgatttttg ccgtgatact gacacgttca
     1501 gaagcggtct ttgcggggcg agcctatgcg gcctatgggg gagtctatat catggcctct
     1561 ctggtatggc tgtgggctgt ggaaggaatg ccccctgacc ggtgggacat ggtcggagcc
     1621 gggatgtgtt tagcgggagc ggcgatgatt ctctgggctc ccagaatggg gtagaaggaa
     1681 ggctaatcat gtctgtcagc tatgcgttgt tttttattag ggaagaagct tttgaaccgc
     1741 ttaaaaaaca aaggttggga gatcggcgta ggtcctagcc tcatggtggt agactccgga
     1801 tttgcaagtg cactcatgag tacaacggcc cgggacgaca tcaagttgga gagggacgca
     1861 agttacgccc gattggatct aggatttttt gagggggtcg cctttgtcgg aaagatacat
     1921 gcccacaagc caagccacca ggacggcaac ataaaattgc ccgacaatcg cctcgagata
     1981 ggtgagggtg cgtgcgatcg gcttaatggg cgtgaggtcg ccatagccta cggtagtaat
     2041 ggtgatatag ctataataca ttaattctga aaattggact cctatggcgg tagatgtcag
     2101 gccttgaaaa gagttcgggt tggccagatt ggtgaataga tagaacattg cccagtttag
     2161 tccgatcagg agataaatgc agatggcgcc aatgatttta ttcacagtga ttgagcctgt
     2221 aagaagcaga tgtcggtaag caatccaggt actgaccaga gaaaacacaa atagaataga
     2281 catgttgacc aaatgaagct caggaatgcc taagaagtag tcgattgtgg cagtcgcgac
     2341 cccgcttact gcaaggattc ctccccctat catcaaccat tttttttcac tgacatggct
     2401 ccaaatcccc atcaggagca tgagacaaag aaccggctct aggaacagac gtcgggtctc
     2461 acccatgact ccttcctctt gagcaagggc aaccgagagc atgaggatca agagacctcc
     2521 taacaaatat ccaaaattca tccttttaat tttcattgat gtcgtctcca aataatgaaa
     2581 tggggaggtt tcatattcat gaacagaccc gggtttcccg gcacttcaat gactcgtatg
     2641 gtcaaacctt gtttaccggc tgatctgaat gtttgacttg tgtgtcccac cggcagagag
     2701 aattttcctc tgtgtaagtc atcgccacaa gatagaggtg attggtttta ggacaatatg
     2761 ccggaatatc gacgtgtggc gatttccgaa ttctttcatc ccatacaaag agattgtgta
     2821 tcagcatctg tgaaaaagac tgtatcccgc ccactcctgt gcggggttga aagggtgagg
     2881 gcggggcgaa aaaaccctcc tttcggtgcc aggcattccg gagagtctat catagaaaaa
     2941 aaaggacagg atccaaaagt tttttaatgc aaaactctag ggaagcgtcc acacgacaat
     3001 ccctgaagac aagcgtgggt ggattcaata gttcctcacg ggaagtaagt gtggtgatgg
     3061 gcctctacta cctggtcctc cgttccatga aatttttttg tgggtaggcg gaaattttga
     3121 gtagggttgc ttctcagccc gaaacaggta ctatagcaac tcttccttag ctgggaggta
     3181 ttcatgagat tactgtattt gtgggaggct ttttatgacg atttctggta aaggtgtttt
     3241 catgaatagg ggatggtatt gggtacaacg aatatgcgca tgggttgtca ccattattct
     3301 cctctcggca tgttcgcaaa atcgtgccta tctctatgag acgggcgaga ggccgatacc
     3361 tccaggcatc gaaaaccaaa tcctccctca taaaacgact caggatgagg tattgactct
     3421 acttggagag ccgattgccc gcctcaaaac caagacgggt ggggggcata ttcatatctg
     3481 gacctattcc tatatggatc tccagagccc ctcacatgac aagggtgagt cattgacgat
     3541 cacctttgat gacaaatcct ttgtggtcct cagtgtcacc agaggcccgc tctaactcag
     3601 gatggggaaa caaggaatat ctcatttgcg tcccttcgga taggcaaatg gattttgagg
     3661 gaactctccc ggatgtattc ttcgcatttg tcctgagcca tggggttaac ttggaaaatg
     3721 cttgaaggaa aaatttccaa tggattggca ggaaatgcct caagcgtcca tttgccttat
     3781 tgccattaaa gggggcaggt ctctgtttga ggacatagca gatcaaccgt ttctctaaat
     3841 ggtatttttc catcctcatg atcatctttt acgggtccaa tccgtgcctg tcatcccatg
     3901 tttgttggct aattgatagt gaaagatatt atcgaatatg ttaaaagcat catggacgtg
     3961 gtggatcgtt acatcggttt ccactcaggg gagaggatgt gtttcccgct agatcgaatg
     4021 agaatgacat ggctgccgca cacaccttaa ttgcctgtca tgaatgtgat ctccttcatc
     4081 gtatgcaccc tctgtattac ggtgagcggg ccaagtgttc cagatgtggt gcatcgctgt
     4141 atgcgaaaaa acgagatagt ttcgagcata ccatcatatt ggcgttggcc agtctcattt
     4201 tctttaccat tgcgaatgtt tttccgttta tgacgtttga gctcaatggc cgtgttcagg
     4261 agagcctgct ttccacaggg gtaatggagt ttttcgaacg ggaaatgtgg gcgttagggg
     4321 tgctggtctt gtgtgcgagt atcctctttc cggccctgaa aattcttggc atgttgtacg
     4381 ttcttgggcc gttggaatta aacaagcgac cgtggcacgc cgctctggtt tttcggatgg
     4441 ttgagcactg ccggacctgg gccatgatgg acgtctatct gctgggagtg attgtcgctg
     4501 tggtgaaatt gtcggatttg gcgaatctgg ttcccggagt cgcgatttat tcattcgtcg
     4561 ccttaatcct gaccttagct gccgccgatt ctgcactgga cacccatgcg gtctgggaaa
     4621 aaatggagga gatttcatga tgggcttgta ccccaccgca gcccggtcgg gactcatgag
     4681 ttgtcatgcc tgccatctac tcagtcccat acctccgctg tcgagtgagg gcgaggccat
     4741 ctgccctcgc tgcgaggccc ctctccattt gcgaaaaccg aatagtattt cgcgcacatg
     4801 ggcattgttg attgcggcct acattctgta tatccccgcc aacctgcttc ccgtcatgac
     4861 ggtgatttcg tttgggaaag gcgaggcgga tacgattctg agcggagtca aggaactcat
     4921 ccatgccgga atgatgccga ttgccttgct ggtattcttc gccagcatca ctgtgccggt
     4981 gttaaagctt ttggcgctga cgtatctgtt gctttccgtt caatacaaat ctcaatggcg
     5041 cccgagagaa cggaccatgt tataccggat gaccgaggtg gtcggccggt ggtccatgat
     5101 tgatattttt atgatttcga ttttaatagc tctggtaaaa cttgatgccg tggcgacaat
     5161 tgaacccggg gcgggggcga tctcatttgc ggcagtcgtt atcctgacca tgtttgccgc
     5221 catgagtttc gatccccgat tgatctggga cagcatagag gagaaaacat gaaggacagc
     5281 gaacccaatc ctggcaattt tgaggaattg gcggaagtga cggtacaaaa aaagcgcggg
     5341 atttctatcg tgtggattat tcccgtggtt gcggcactga tcggagggtg gctgacctac
     5401 aaaaccatct ccgaaaaggg tcccacggtc accataacct ttgaagacgg ggcgggattg
     5461 gaagccggca aaacgaagat taaatataaa tcccttgaaa ttggtaccgt caagcgggtt
     5521 gaaatcagcc aagacctctc tcgggttaat gtgatagccg agtttcgcaa accagccgaa
     5581 ccccacctca cggaaaattc caggttttgg gtggttcgtc cccgcctggg tttaggcggc
     5641 atttcagggc ttgagacgtt agtgtccgga gcctatatcg ccatggatcc caggccggga
     5701 ccttcggctc tgacatttac gggattagag aaaccacccg gagtgacacg agaagacaaa
     5761 ggggctcaat ttcaattgcg tgcggaaaac ctgggatcga gtgcccccgg tgcgccggtc
     5821 ctttatcacg atatccaggt tggacgtgtt cttgattatg tcttggatga ggaaggcgag
     5881 ggcgtgttta tcgatatttt tattcaagac ccctatcatc tgcgtgtaga agataccagc
     5941 cgattctggc agttaagcgg gtttgaaatt tcggttgggg cggagggttt gaatgtgaaa
     6001 atggagtcgc tctcctcact ccttacagga ggaatcgcgt tcgatacccc ggcaatggca
     6061 ggaggctcgg gggaaccgag ccaaccgggt acggcattca aacttttcag gaatttttcc
     6121 agtattcaag acgagaaata tgttctgacg cgtccgttca tgatcaattt cgatgggtcg
     6181 gttcgaggct tgagtgttgg ggctcctgtg gaattccgtg gcattaaaat cgggatcgtc
     6241 tccgatatcg cagtcaatct cgatcccaac acgcttgata ttaaaattcc cgtactgatc
     6301 gagattcagc cggaacgaat taccacgaca caagtcctgc aatcacaaat catgcaagcc
     6361 caggtttcag atgatcgata tgctgtcatg aagcatatgg tgaaacgtgg cttgagagcc
     6421 caattggaga ccgggagctt actgaccgga caattgtttg tcggacttga cttccacaaa
     6481 gatcttccac agaaagagct gaacctgact ggaaaatatc cggagattcc agccataccg
     6541 gcaaccatgg atgaattgcg gcggaccgtc accgatgtga tggccgagat tcgacgcttg
     6601 cctcttgata aaattgccaa ggaaattctg gagacggttg aaggaggaaa ccgactcgtc
     6661 aactcccctg agacgcaaaa tgccattcat aacctcaatg ccgcattagg aaatgtggaa
     6721 aagctcacag gggggcttga ccagcaagtt gatggtctga tgatgaatct ggacaaaacc
     6781 ctggggaccg tccagaaagg gctacggata gttgatcctg attcacccgc tgcggtgaat
     6841 ataaataata ctctgaagga gctgtctgct gccgcccgtt ccattcgggt gttggccgat
     6901 tatcttgaac aacatcccga ggcattagtc aaagggaaac aatgaggtgg tggacatgaa
     6961 cagggtcata aagggggttt ttttattttt tgcttgtgta ggcgtgctca ctctcctcgg
     7021 gtgtggaacg agccaaccat cgcatttcta tttgttgcga gccgtgtcgc ctgcctcggt
     7081 ctccggccta tcggacacga aaccatcgag cctgtccttt ggactagggc cgatcaccct
     7141 tcccaaatat ttagaccggc cgcagattgt gaccaaaacc agtgcgcatg aagtcgagtt
     7201 ggcggaattt cataaatggg ctgaaccgtt gagtgagaat gtgtctcatg tcctggccga
     7261 aaatctttca gccctacttt cgtcggatcg aattctacag tatccgtgga ggagttcaat
     7321 tcccctggat taccagattg ccgtggatgt cttgcagttc gatggcacca tgggagggga
     7381 ggctgttctg gtcgcccggt ggagtctgtt gggtggggat gaacaaactg tgctcaccac
     7441 taggaaaacg cagatgaccc atgctccgac cagtcagaat tatgaagcgt tggtcgaggc
     7501 catgagtcgg aatcttgagg atctcagtcg cgaaatcgca gaggcgctca ccactctttc
     7561 accaaagatc cagtcccggt cgttacccta atcttctgtt gaggtatccg aatttctttt
     7621 gagtgttctc ttacaactgg agtttgacgg tcgcatcatc aatcctacat gctgcccagc
     7681 tcaatacact gaggtcatcg ctctttttca atgattgcta ataatagtca atggctttga
     7741 cccgtaacga tttttcctgc ctggcagatc tgatagcgat taccacggaa tcattcatcg
     7801 cggacaggca gaggaaaagc aggcgtcatt cttatctatc gtattgagag taagacaata
     7861 tgaatgacgg aggattcgaa gacaacgggt tcaccagacg aaatttatta cgggtccttg
     7921 ggatttcggc gggaatggct tccctggccc atcttacggg atgtggaaac ctttgggaac
     7981 ggcagcccgt tattccggtg gacccctgga ataagggagt ctgccgattt tgtggaaccg
     8041 ggtgtgggat gctgattggc gtgaaggagg gaagggggtc gtcgatgtga agggggatga
     8101 atatgcccac aaccgtggac gcctgagtat taaggggctg atgaaccgtg aaattctgta
     8161 tggaaaggtt cgagccctcc accccatgat taggaaaaat ggaaaacttg agcgtgcgac
     8221 aagggaagag gccatggact tggtggcaaa gaaatttcgt caggccatcg acgagttcgg
     8281 gccggatagt gtggcctatt acgggagcgg ccaactctat actcaggaaa gttatattgc
     8341 caaaaaatta ttcaaggcgg ggattggcac tttaatcagg aggcttcgga gacccttatt
     8401 cacaaaagcg aatgagggat ttggaagcta aacgtctttt gtaatggcaa aatttctgcg
     8461 aggtattgga aaaactaacc aaatatgact gggccaatgc ccgtctcaag tggaggaatt
     8521 cataaaaaca gcacctttca gacttctgag gggaggaatg cgcaaatggg attaacctat
     8581 ccaatgaaaa tgggctcccc agctttctag gggagagcct cacttttaaa atcacaagcc
     8641 ggttttccct tgccctctga ggttgcgcca tgttcattct gggccaggcc ggaagcatct
     8701 tatatcttat cgccggcaga actgcctcga cgaccgaccc gataaccgga cataaacaag
     8761 tcagtcatgg cgcccgggaa tattccgatt atgagcaaat acaggcaccg tccaactaac
     8821 cggctaaaaa actatcaatc aatgcagtaa atacgccatg aatcaggggt acgccaagga
     8881 gcccctgtgt cacactgcgc atcgtgggcg gcaggttctg cgagttaagg tgtgtggaat
     8941 aataaagatt ttcaagggaa aatttgccct tgattagatt attcaaaggc tccggatcag
     9001 ttcaaaaaag ttcgtccagc aaggccgcag ctgttttttc gcgctgggag cccagggtcg
     9061 ttcgatctgt gttagctttt ggagagtgat aatccgggag gaataaagaa gggcgattct
     9121 ttcctcaatt ttatagatcg attgtcgatg ggcttaagca cgcaacttat aagaagaatt
     9181 tcatgtatcc ttagattgct ttccctggtg aggccaatga aaatccacct gcgaatcggg
     9241 tgtgtcatcg cagagaacaa aaaaggatgg gtgaaaaatt tatatcacac aaagagagga
     9301 gccatgtgcc cactttaccg gagtcttttg atggatgccc ctcaattagg tggtcatcgg
     9361 atttttccga tgaccttttt cgaaaggggg gtcatttgag ggcttcggct cctcccacaa
     9421 tatccatcag ttccttggta atggcttcct gacggctcct gttatacttg aggctgagtt
     9481 gcttgatgag ttcaccggcg ttgcgagtag caccatccat ggcagccatc cttgcggatt
     9541 gttcggcagc agatgattcc aacaagatgc ggaatacctg accctgggca tacttgggaa
     9601 gcagtgttga cagcaaatcc tcctgatcgg gttcatacag atagccgccg ccggatggag
     9661 cctcagccgc tccgaattct ttcagcggtt cgatagggaa gactttatcc acgacaactt
     9721 gctgttggac gacgttttta aactcgttgt acacgatata tccttcatca aaggcccctt
     9781 cggtaaacgc ctctacggca gcccatccga cttcgagtgc atgctcatag gtcaatcggt
     9841 caaacacatt cacattcgga tcgcgaaggg gccaatgacg gcggcgaaaa tactcgtttc
     9901 ctttccggcc gaccactccc acgatgacct tcgcccctga ttcttcgcat ttctggatca
     9961 tctggagagc tctgcgaatc acgttcatat tgaacgctcc gcacaatcca cggtcactcg
    10021 tcacgacggt cacttgaatg atcttgacct ggcgtttttg gaataggggg tggatagatt
    10081 gattgacccg accactcagg ttggcaagca cacggcgtaa ttcttgtcca taggggcgaa
    10141 acgccagaat tcgctgctgt gcacggcgga gtttggatgc agccaccatc ttcatggcct
    10201 ttgtgatctt ttgtgtgttc ttgaccgagc cgattttacg gcgtaaggcc tgaagatttg
    10261 ccatccctgt tcctcctaca tcttgaatgc gatcttgtga attgaatcat gagtttccta
    10321 ttttttttct tacagtcttc tcttgaattg tatgccttct catcctgatc aggatctgac
    10381 gaagaaaggc ctgcgaacca gaaccgcgtg ccctccacat cgggttctta aagccattgc
    10441 tccctttttt aaggggtccc atgacaagtc ggagagtcta gcaagttcaa ccggcgatta
    10501 tgatcccatg aggcaggaat gcagaaaagt attgtgcatg gaacgaggga atgttgcaag
    10561 aggatttggt tgaaaaaatt aaagggtttc cctcacaggt acagaaaatc cggttcaaac
    10621 ttaaatttca tgatggccgg tagatagagc caaccttttt tctgtcgccg acttccgctc
    10681 gtttaacttt tcagttcagg ctgcatttca tggtctattg gaccaggttc tgaaggggag
    10741 gaaacaggct gcccctcttt atcggtcggc gaccagccgc cccctagggc tttgtacagc
    10801 cggacaattg aactcaggtt ttgctggcgt gttgagacaa gtcccaattc ggcctgaaag
    10861 aggtcccgtt gggctgttaa gacatccagg taggaggcga accctccgcg ataccgtttg
    10921 tccgcgaaat tcaaagcaga ctgtaacgat gagacctggg attcctgcgc ctcacgttgt
    10981 tcgccagttt ttttgatggt gatcaacgaa tcctcaactt ctctaaannn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnncac gttgttcgcc agtttttttg atggtgatca
    11161 acgaatcctc aacttctcta aaggcattca ggatggccaa ctcgtaccgg gcaagagcct
    11221 gttgttcctg ggaggtcgcc accccgacgc cgtagccaag cgcggttgca tttaacagcg
    11281 ggccggttaa ggtgcctgag acgttagcag cagcaaaggg tcccgttccc atcgagttta
    11341 actgaaagcc ggccacccct cctccggcat tgatgctgaa ttggggaaag cgttgagcct
    11401 gggccacacc gatatttgcg gtggccgcgg cgagtttttg ttccgcttcc agaatatcgg
    11461 ggcgttgact caaaaggctg gaaggcaacc cgaccggcac ttcgggcggc agaggctgtt
    11521 ccgtcagcgc aaatccgcga ggaatcgcca tcgggcggtg tcccaacagg acgctgatcc
    11581 ggttctccgc tagcacgaca tgctgttcca gattggccaa ttgtgctctg gtgccggcct
    11641 gttcggcctc gaagcgatcc agatccagtt tagaagcata cccgtgttca tattgtgacc
    11701 ggctcaacct cacagcctca ttccagactt tgagcgtacg ttcggcgatc ttcacctgaa
    11761 gatccagagc ccggagggtg aagtaggatt gcgccacgtc gctgaccagg gcaatgacca
    11821 caccacgctg gttttcaacc cgtgctaata attctgcttg ggtagcttcg acattccggc
    11881 gtatccgtcc ccaaagatcg atttcccatt tgatgccccc aaagaacttc tccctcgagt
    11941 aatcaagtcc ccctgatttt ttgtttccac cgtctggggt cgggatcagc ccgcttccgg
    12001 tttccagccc tttattattt gtgttgtgga agtaaagcgc cttcccctca taccccaagg
    12061 aaggggcaag gtcccatttg gcgataacga gttggttgtt gaactcctgg accgccgcgg
    12121 cggccacccg aaggtcaaga ttttcctgta aggcggtgtg aataagtttc tgaagttctt
    12181 catccttgaa caattcccac cagggcatat tcgcaataga ttccgaggtt ccaggggcga
    12241 gccgccagga atccgaggtg ggaacgtccg ggcgtttgta atccggaccc atggtgcagc
    12301 cggagaagag caggaggctc agaacgatgg ctccgcattt gttcatatcc gcgatctccc
    12361 tagagcttga aaggagttgg tgcatttttg ggttggggga tgactccaac cgcatcttcg
    12421 tcttccatga attcaggatc cacaaaatca tggtcgtcgg ggtctcggtc agggtggtcg
    12481 tcgtggcggt ggactaatcg ctccaccacg taaaacgaga ccgggatgag gaaaatcgcg
    12541 ataaagcttg ccgcgatcat ccctccgatg acggtgacgc ccagaaggat tctcccatga
    12601 gctccggatc ccgaagagag aacaagtgga atcactccga agataaaggc gaaggatgtc
    12661 atgaggatcg gccgcaatcg caggcgggct ccggtcagag ccgcatcgac gagagaacga
    12721 cccttttcat actccatttt ggcaaattca acaatcagga tcgcgttttt cgcggacagc
    12781 ccgatgagca tgacgagacc gatttgggtg aacacatcgt tttccattcc gagtgcccac
    12841 aaggcgccga aggcaccgac gactgcaatc ggtgtgcaga tgagaacact gaacggaagc
    12901 ccccaactct catattgggc cgcgagaatg agaaagacaa agagaaggga gaagccgaag
    12961 atcacgctgg ccgggactcc ttcggaggca actttctcct gaaacgacat tccgatgaag
    13021 tcgtacccca tctcgcgtgg catcgtttcg gcgaagactt cttccagggc ctgcatgacc
    13081 tgaccggagg taaatgcggg attcgctgaa gcgaaaacct gcgcggatcg gtagccgttg
    13141 aatcgcatgg tgaattccgg accagaaatt ttttccattg tcaccagtgt cgagagaggg
    13201 accatctcat cggatttatt tcgcacgaaa aattgcccca cgttttcggc cttggtcctg
    13261 aattctcctt cagcctgcac atacacctgc cagacccgcc cgaaccggtt gaaataattt
    13321 accattacgc cgcccataaa ggtctgcagg gttttataga catcttcaag tttcacccct
    13381 tgcttcagca ctttgtcgcg atcgacatgg gcaaagacct gtgggacgtc ggggatgaag
    13441 gtggtgttca tggacgcgat ttcgggacgc tgtcgtgcgg cttccatgaa cgtgttgagg
    13501 ttgtcggcga ggaaggccac atcctgtccg gaacggtctt ccaggatgaa ggaaacgcca
    13561 cccgatgtgc cgacgccggg aatggccgga ggcgggaaaa gaaaggcgtt cgcttcgggt
    13621 aaggcggcga gtttctcgtt cacatttttg aagatcgcct gaagcttttc ctcgggtttg
    13681 gtccgttcat cccatggagc gagcgtgacg aaaaagaacc cgttataggt cgtattgacg
    13741 gtgctcagga gactaaaccc tacgatggtg ctcacaaatt gaattccggg agcttctgaa
    13801 agaatggctt cgattttttt tgcaacctca tccgttcgtt ggagagaggc ggccaacggc
    13861 agttggacgt ttaaatagac atatccctga tcctccatcg gaagaaaccc ggtgggaagt
    13921 ttggttccca acagaccggc acataccgca gccacgacca gaaagatcat cgcacggccc
    13981 gactttcgga tcaggtagtg gcaatacccg acatacccgt ccgtggcccg tccgaaccaa
    14041 tggttaaacc atccgaaaaa tgcccccaac gggcctctgg ctggagttct gggcctcaga
    14101 atcattccgg caagcgcggg gctgagggtc aaggcattga aagcggagat gatcaccgat
    14161 aaggcaatcg tcagcgcgaa ttgttggtag agccgccccg taatgcccgg aataaacgcg
    14221 gtgggcacga agaccgccgc gagaatcagt gcgatcgcga tcaccggccc ggaaacttcc
    14281 tccatagctt tccgggtcgc atccttgggc gacaggcctt tttcgatatg gtgttcgacg
    14341 gcttcgacta ccacgatggc atcgtctacg acgagcccga tggctaatac cagtccgaat
    14401 aaggagagcg tgttgatcga gaaatccatc aatgggaaga tcgcgaacgt ccctatcagc
    14461 gacacaggca cggccagcag aggaatgagc gtggcccgcc atccctgtaa aaaaaggaag
    14521 acgacaatta tgacgagcac aattgcttca aagagagtat gcacgatctc cttgattccc
    14581 tctctgacag ccagtgtcgt atcgagcgag atggcgtaat caaggtcttc gggaaaccgc
    14641 tctttcatct cctccattag cttctttgcc cgatcaaccg tatcgatggc gtttgacccc
    14701 gggagttggt agacggcaat gagcgcgctg ggctttccgt tgaaacggcc gatcatactg
    14761 taggattgcg cacctaattc cacccgggcc acgtccttca gtcggatgat agaaccgtcg
    14821 ggatcggccc gaacgatgat ctgtccgaat tcttcctcgt taagcagacg accttgtgag
    14881 cggaccgtat acgtgaattc ctggccttgc ggaaccggtt gcgctccgat ctgaccggcc
    14941 gggttgacgg tattctgctt cttgatggca tccaagatct cggtcacggt gatgcccagt
    15001 ttggcgagat gatcgggttc cacccagaag cgcatggcat actggccggc tccaaagatc
    15061 gtgacctggc cgaccccttg gctccgcgaa agaggatcgt tgatgttcac gtaggcatag
    15121 ttagccagga agatttcgtc ataggtgcca ttcggggaat acaggctgaa gagcatcaga
    15181 ggactggtgg tggacttttt gatggtcacc ccgtagttct tcacatctga gggcaattga
    15241 gattcggcct gcaggtaccg catctgggtc aggatctggt catcgttggg gaccgtgttt
    15301 acgtcaaagt ccacgcgcaa cgtcatctgc ccattactgg cgttcgtgga gtacatgtag
    15361 atcgagttgt caacaccggc catctgctgt tcgatggggg tggcgaccga ttgttcgacg
    15421 gtgacggcgt ctgctccgac gtaggtggcc tgcagttgga tttccggagg cgcaatgtcg
    15481 ggaaacaggg cgatgggaag ttgcaccatg gcgatcagcc cgaggatcgt catgatgatc
    15541 gaaatgacga tagcgacgat gggccggtcg atgaaaaatt tagccataag acagcttgtg
    15601 attatgtggc gctaaccggg tctggggaac tctcagcgcc gggtggtggt tcgtccgaag
    15661 ttgcttttgg cgtgacaacc ataccgtctc gaaccttgag aagtccctcg acgatgacca
    15721 tctcgccggg atgaaggccc tcgtcgacta gccagagaga tccaattctt ggccccaggg
    15781 tcacttttcg aatcgctacc ttgttttcct ttccgatcac ggcgacctca aaggtaccct
    15841 ggagttcctt gactgcccgc tgttggacta acaggcccgc ggttcgagtt tcgatggggg
    15901 tacggatctt ggcaaaaagg ccggggcgca ggatgttgcc ggggtttggg aaataactga
    15961 cgatggtgat cgtcccggtt ttgacatcca cctgacggtc ggccaggacg gctcttccct
    16021 tgtgtgggta tatgctccca tcagccagaa ccagctcaag tcttttttca tgggaagggt
    16081 ttaaattttc ctgaccgccg gccttttcaa tccgttccgc cagttttaaa tattcctgct
    16141 cgctaatggg aaagttgacc ttgatggggt ccaactggga cacagtcgtc atcaacgtat
    16201 tctcgacgat gagatcaccg atctgggcgg tggcgatccc ggctatcccg ttgatcgggg
    16261 aggtcacgct cgtccagttt agattcagtt gcgcattctc cagtttggca cgcgccgaag
    16321 cgactgaggc caggttgact gcgcttgcct gaagggcatc atccaattct ttttggctga
    16381 tggcgccttc tgctgccagg ggagtgttcc gcttaacgtc aagctggctc tttttatagg
    16441 ccgctcccgc acgggccagt tcggccttcg cttggtccag agcagcctgg tatggtcgtg
    16501 gatcgatttt aaacaacagg tcattggtcc tgaccgccga cccttctgtg taatgacggg
    16561 acaacagata cccctggatt cttgcacgaa tctgggcgtc cacatacccg acggtcgttc
    16621 cgacccattc cccataaatc gggacgtcct tttgtattac ctcagctacc aacacctcag
    16681 gtggcgccgc cgtggattcc gcctcttttc gatcacagcc caaagttatc cccaatagaa
    16741 ggcagaccat gaggcttgcc actccccgaa agtcccgacg ggcggtttgc ggttgtgccc
    16801 tcacgataaa aggccttggg gcctggaaaa aagcattgaa acgatggatg gtcatactcg
    16861 tacttcctat ggagggaaga aaatgaaacg gttgtgattc tttcatgttt cgagttccat
    16921 tctcaagtag ggatcctaaa tatattagtg ttgttttttg aaactggctg gtgaattcac
    16981 atttgagggc agcgtcctga aagagttagc aaaagttttt tcggtacttt ccatttgtga
    17041 atgtagccat ttcgaacgat cgatggtctt catttgacgt cttattcact gctaaatttg
    17101 aattttcaaa acttttctgt atggggatat ttcatcaaca aaaaatggcc agggactgaa
    17161 accgattgtt ttaaacgtac aaaaccttta aatttactcc ctacatgacg catcttcctg
    17221 gaggtagggg cttcccccaa aaggattgga ccgatctcgg acaaacggac acagatctca
    17281 caagagatta accttcaacg gagcatctcc ctatgacctc tctcactcaa cgacctgttt
    17341 ggaaatccct tgaacaacat tatcaacagg ccaagaaact tcatctgcgc gccttgttcg
    17401 cggatgatcc tggaagaggt cgacggatga ccgtggaggc tgaggggatc tacctggatt
    17461 attcgaaaaa tcttatcacc ggagacactc tacgcttact ccatgaactg gcggaaagcg
    17521 tgaatttgcg gggccatatt caggcgatgt tccggggtga caaaattaat atcacagaag
    17581 accgggcagt tcttcatgcc gccttgagag cctccagaaa cgcatctatc ttggtggagg
    17641 gcagaaacgt ggtgccggat gttcacgcgg tgttggatca aatgggggat ttttctacac
    17701 gagtcagaag cggggaatgg aagggctata ccgggaaacg catccggaat atcatcaata
    17761 tcggcatcgg gggttcggat ctagggccgg tgatggccta tgaggcctta aagcattaca
    17821 gcgaccgcaa tctgaccgtg cggtttgttt cgaatatcga tggaacggat ctggctgagg
    17881 ctaccagaga tctcgaccct gcagaaactc tttttgtcgt ctgttcaaaa acctttacca
    17941 ccatcgaaac cctgacgaat gcgcaatccg cccgagactg gtgtctcaat gctcttcatg
    18001 acaaacaggc tattgccaaa cattttgtcg ccgtttcgac aaatcgcaac gaggtccaga
    18061 aattcggaat tgacccggcg aatatgttcg aattctggga ctgggtcggc gggcgctatt
    18121 ccctggattc ggccattgga ctttcgctga tgatcggggt cgggcctgat cattttcgag
    18181 acatgctcgc tggatttcgg gcgatggatg agcatttcca atcagcacct ttcgagaaaa
    18241 accttcctgt gcttctcggc ctcctgggtg tgtggtacgt caactttttt cacgctgaga
    18301 cccacgccat tctcccctat gatcaatatc tgtggcggct cagcgcctat tgtcagcagc
    18361 ttgacatgga gagcaacggc aagcgcgtga ctctagacgg gacggttgtg gattatcaga
    18421 caggcccaat catctggggc cagcccggca ccaacggtca gcatgcctat tatcaattga
    18481 ttcaccaggg gacccggctg ataccctgtg actttatcgg tttctgccgt tccttgaatc
    18541 cctccgggcg gcaccacgac ctgctcatgg ccaatttttt cgctcaaacc gaggctctcg
    18601 cattcggcaa gaccgccgag gaggttgaaa ctgagggagt ctcgcaaacg ttaattcccc
    18661 atcgaacttt tcccggcaac catcccacca atacaatcat ggctgaggaa ctcacaccag
    18721 ccatgctggg aaaactcatc gccttgtatg aacataaagt gttcgtgcag gggattatct
    18781 ggaacctgaa ttccttcgat caatggggag tggaattggg caaagtgctt gccaatcgga
    18841 ttgctccgga actgaccaca ccacgggagc cggatctggg tcatgacagc tccacgaatg
    18901 ctttgataca acgctatcga cagcaccgtc aaatccaggc ttgatgctgc cattttaagt
    18961 cttaaaatct tccaaaattc atttcttttt aggatcggaa aagggacagt tgaatataac
    19021 cgtgtggggc tttctcgtgg agcggtgacg gccaaggcca tcttgtgaaa gggccacgga
    19081 aaaatccaat caatactcat cttcaccttt gccggtacgg tttttgatat tttcgatcag
    19141 ccttttgagg tagaatcgat tccgaatcgt ggcatgcagt tccgaatgtg tttttcttaa
    19201 attaaggagg gctgaagtta tgcgacagag tgtggagaga acaattttga ttctgttcct
    19261 ctttgttttc tttctgcagg ggtgtgcggc gacacaacaa cggcgagatg tggtcgaatc
    19321 aggatttctt acagagggtg agcatggcat gctcacggaa ggtgggagca acgaagcact
    19381 ccttcgttac gtcaatccgg atgtcgactg gcgctcgtat gacaaaataa tgcttgattc
    19441 cgtgtcagtc tggaaagacc ccgaaacaca agatgtgtct ccggaagatc tgaagaagct
    19501 gaccgatttt gcctatggcc aactgcatga cgctctgagt ctggactata cctttgtgac
    19561 ccaacccgga cccggagtca tacgtgccac ctttgccatc acggaagcgg aggcttctaa
    19621 cccggttgca gatgtggtga cctccattat acctcaaacc agaattttga ctggagtcaa
    19681 agggcttatc gtgggaggta agcctgggtt cgtaggaaca gccggcttag aggccaagtt
    19741 taccgacgcc acaaccggga gaattctggc tttggccgtt gaccggcggg gcgggaccaa
    19801 gaatctcagg gggatgacta acaaatggaa tgatgtggag aaagcctaca cttattgggc
    19861 gtcggctgtt cgctatcgat tgtgcatgtt tcgtggcgac gcgaattgtg tggaaccgga
    19921 gtcctaagga catcattgtg tggaaaacga gggaagttaa gagagagtgc gggatctctt
    19981 tctcagttta tttataaatt ccagtgaatt ccctctcaca caacgtcaaa tgtagtgtga
    20041 atgtgttgcg agtgaagaat agaaagccgt cccgggtttc ctccggaaac ccgggttttc
    20101 ttattcgcgt catttcggaa aaggtctatt gcagagttcc ccgctttagc cattcccggc
    20161 attgatgatc gggaatgcat cctggattcc tttcggatga cccgccacaa gctttgcgta
    20221 tgacccaagg agacaggaac ctctgccacc gatacgccgg catgacaaag aggggggacc
    20281 tccccctacc tgccggcaga gatgacgatt gcaagaaaga aaaatcggtg gggaaggaga
    20341 agagactgcg agcttggttg gtaaattttc cgcagacggt aaatggcttt ttccccgctc
    20401 cacttcttct tatggattat gcgtcttgac tgatgggtag caacacggat caaccaacat
    20461 acaattctcc ggtgggtgac atcgaagggt ttttgtaatc ggatagctgc taactggaac
    20521 tgcccggagc aggccaccat tgaggcagga aatccgcgaa tgggatcgcc ttttcactca
    20581 ctcaggcgaa ggagaaaagt gaaaggaagt tggccaagaa aatacaaaag ccagttgcga
    20641 acttcatcaa caatttttcc tttcaaagca catgaatttc cggctggggc ctcaccaact
    20701 gacacaaatc gtgttgaact ttcagccggt tatttccgtt aatcttacct gatgaataga
    20761 acctgagtta cgcgcgcgat cctgccggtg atcaaacaac cgggtttccg cacagcagcg
    20821 atacccggag aattggagat ggcaatctgt acctgttttt ctcaagagac gccttatgca
    20881 gagtgcttgg gtgatgaggt tcacgggaaa tgtcttgaat gagagtcttc aaccaacagc
    20941 cagaggtaag aacatgaatg tacggttatg ggtggggtca tgttttctcc ttctgtcagc
    21001 ctgcggccct acacagcaat gggtgaaacc cggggcgaca gaggcagact tacgttcaac
    21061 gattactctc tgcgaccggg agaatcgtca ttttaatcag gaaggcatct ctcattatgg
    21121 cccggaagat gaaaccgtcc gaccaagaaa ttatcgcagg ggcggagggg atatgatgag
    21181 ggaacaatgc ctgcaaagtc acggttggac acttgaatat gtagagtgaa acggtaggtt
    21241 caaaaaccgt gaaccaacag gccttcagcc ttgattcaat tcatttcctt gactttgcga
    21301 cggcattagg gtacgagcaa tctttgctgt gagcagcgtc tagtgaacca gatatctccg
    21361 gattattaac ccgcgagcac aaggagggac ccgcatgcga tttctgattc agaaaaattt
    21421 tgttggcctg gcgatcttgg tgttggccat gcctgccatt tttccgtgct cacgtactgg
    21481 aagtatgctc ttcgcgcaaa aatgactgcg gccttccctt cgatttgact cagggcaggg
    21541 ctggacaaac ccttcgaaag gctcaggaca tgcttttatt gaaccccccc gaggcctctg
    21601 atatgaaccg tgcctgtggg tcaatatccc cttggaaatt ataattattc aacactccct
    21661 gtagagccca gatcatcgcc ggtggtgttg attttgttga tgctggcacg cgtacctccg
    21721 atacatcatt tttcgagtga aataaagttt ggtgcgatcc tcacaaaatg ccaagtagtt
    21781 tcctaggaga cgatgcagga gagattcatc cagggtgatt aaatggttga cgctcttccg
    21841 atattgatgc aatgaagctc ttcaatcttt gagtggcatt tcctcctctg acttggagga
    21901 attcgtcagc aacatccgga gaaaatgcag ggttttcaat ttgtagttgc gggaatcggt
    21961 ttcctccccc attcgcgaag ccgttgggct aacttatctg cccaatactc aaatgcctgg
    22021 tgggcgtcgt cccaggaatc tatcgaacca tccagagttc tgccaccaac ccgacgatcc
    22081 gcagcggcca tcaatagggt tcccgtgttg ccatcggtca atttcgcctc gacgctggcg
    22141 cgacctacaa atgcctgtgt ccctgtggcc agtttttttg caccggatac catggccgca
    22201 ggaggaataa tcgtcgatac gatatccatc ccgacgtcgg atggctcagc ctccgtcagg
    22261 gccagttcta tttgcaatgc acccggaaca agtttgggta ccacaagaaa atcttctttc
    22321 agtttccaaa tgacttttga acgtagatca tccgctaacc ggtgtaattc ttcctgtggt
    22381 acggtggatt gtttggatat caatacgatg gccggttcca catacacggc ccggtaaatt
    22441 gaaaaatcag cttgagggtt tttgtacaac aggagagctt ctccctcctt tcccggttgg
    22501 agaatcgaat aatcttcgag gaaaccagag agttccacct ccttagcttg ttcggtttga
    22561 gcacatcctg ctaccccggc cagaataatc agaatcgaca ccattacatg ccattgtgca
    22621 aatctaaggt ttcgttgaaa tggtaaatgc cgttcttctc catccatatg agatgttcga
    22681 tggggagtgg agcttgtctc atgtttcttc atcagccatc ccttctggtt cattttgtaa
    22741 gcctataggt gaaaaagggg ctgtgattaa atgcttcttt taattttccc ctgattctga
    22801 ttttttatgt tcgtgagtaa aacacgattt ccctcaataa gtcattgcgg ggatttctat
    22861 cttcctgagg acttggcggg tgcttaacgt gaactctcct tgggaaggat gcggaggctg
    22921 accatacaga caccaccgaa cagcttctgg attaaaccga atgtatggtt ctggcccaac
    22981 accttgacaa tctatatcgg gaattccctg tggggtttac caataaccat cgccgggagg
    23041 tgaacgtctc gcgcgtgtgg ccccaatcct cgtcgcaact taatggtttg tatgaccggc
    23101 tttcgcccag gcaggcgtcg ggcaaaggcg cacttatgta aagagtgggg agccatatat
    23161 attctttaga atcccaactt cttgattcgt cctgtcatga gtgggaagac tgttattttt
    23221 tgagctggtc tttcgccgca tcgacgatgt gttcaaccgt aaatccaaat ttcttggtga
    23281 gttccttgag gggagccgaa gccccaaagg tttccatacc gatgatccgt ccggttgatc
    23341 ccacatagcg ctcccagccc agggtagaag cctgctccac ggaaacccgt gcggtcacct
    23401 gtgcagggag caccgtttca cgatactcgg gatgcttccg gcaatagtac tcaaagatct
    23461 cccatgaggg catgctcaca acgcgcgcat taattctctc ggctttgagc cgctcggagg
    23521 cttccacgca cagagatact tcactcccgg ttccgatgag gatcacatcc ggttccccat
    23581 ccatcgtatt cgtcagggta tacgcccctt tggccacacc ggaggctgag gcatagtgag
    23641 tccgatcaag tgtgggaatg gtctgtctgg tcagaacaag tgcaaccggc tcatgacgga
    23701 attgcatgat cacgcgccaa gcttcgacga cctcgttggc atcggcgggt cgaatgataa
    23761 tcagtcctgg gatagctctc agtgaggcga tctgatcgac tggctgatgt gtcggaccgt
    23821 cctcgcccac accaatggaa tcgtgcgtga aaatatagat gacgggaatt tccatgatgg
    23881 ctgacagtct catggttggc cgagcgtaat cgcagaagac caggaaggta gatccgaatg
    23941 cgcgaacctt tgagagtgcc agcccattta tcacggcccc catcacatgc tcgcgaatgc
    24001 cgaaatgcaa attacgtcct tgataggtgc cggacaaaaa gtccccggct ccgtcaaagg
    24061 tcaggcgtgt cttgacagac ggaccaagat cggcggatcc cccgatgagc cacggaatgt
    24121 gttgtgcgat gctattaagc gtcttccctg aggaatcccg ggtagccaac cctttggcgt
    24181 ctgccgggaa tgacggtaat cccttatccc agtcttcggg gagttgacga tgctgcattt
    24241 tgtggagttg atcggcaaga tcgggatagg cttgcgtata ggcctcgaac cgggtaaacc
    24301 agttttcccg aagggaccgt cctcgttttc ctatgccttc acggaaatgg ccgggtactt
    24361 cttcgggaac caggaatttg gcatcttccg gccagccata tcgttgtttc gtcaaccgga
    24421 tttcttcttc accaaggggt tcaccatgtg cggcactggt gtcctgtttg ttgggcgccc
    24481 cataggcaat gtggctatcc acgataatca acgtcggccg gtccggagtg gcaaggaagg
    24541 tgttgaatgc tcgagtcagg atgtccaggt cattggcatc tcctacgcgc gtgacattcc
    24601 agccgtaccc aataaaccgg gtggcgacat cgtcgctggt cgccaattcg gtatggcctt
    24661 ctatcgtgat gttgttgttg tcatagatcc aacacaaatt ggagagtcga agatgtccgg
    24721 caagggaggc agcttcatgg gaaattccct ccatcatgtc tccgtccccg cagagcgcat
    24781 agacgcggta atcgaagagc gggaaaccct cccggttgaa ctgcgccgcc atccagcgtt
    24841 cggcaatggc cattcctacg ctggtcgcca caccctgacc taaaggaccg gtcgtcattt
    24901 ccaccccgga ggtccaccgg tattccgggt ggccgggaca tttactctcc aattggcgaa
    24961 accgcttgat gtcgtccagt gtcaccgaga gatggccgag ccgttcgtat ttggcattga
    25021 cggttttgac cccacagaga tgcaggagag agtacaacaa catggatgca tggccgatgg
    25081 ataagacgaa gcgatcccgg tttccccata tcggatcatc gggatcgaac cgtaggaagc
    25141 gattccataa acagtacgcc accggcgcca gcgccatcgg cgtgccgggg tgccctgaat
    25201 tcgcggcctg gacggcatcc atggacaagg tgcggatcgt attgatacag agttgatcaa
    25261 gttgtttgtc cggcatcatg tcagtgctcc aagaggtctt ttagaaattc tccgggatgt
    25321 acgatctttt ctgcatcgcc gcttgatcgt cgtactgata tttctttgat cgtgcgggta
    25381 gttttacttt gtcccgcttc acccgtttat agggaatcga gctgagcaga tggctgatgc
    25441 agttgaggcg cgcgcgtttc ttattgtccg atcgcacgat gtgccagggg gcatgtttgg
    25501 agtctgtggc atccagcatt ctgtcgcgcg cgcgcgagta ttcgtaccat ttctcaagtg
    25561 acggcaggtc catcgggctg agcttccatt gacgcaccgg atcttcgaca cgggcctgga
    25621 agcgccgctt ttgctcctcg tgactcactt cgagccagta tttgatcaaa atgatcccgc
    25681 catccacgat gtatttttcg acttccgggc agaggtggag gaaacgctcg tgttgttcct
    25741 tggtgcagaa gcccatgacg tgttcgacac cggcccggtt gtaccaactg cggtcccaga
    25801 tcacgatttc accggatgcc ggaaagtgtc ccatgtaacg ctggatgtac atctggcttt
    25861 tctcgcggtc ggagggtgca ggcagcgcca cgacgcggaa cacgcggggg ctcacgcgtt
    25921 cagtaatggc ccgaatcgtg cctcccttgc cggctccgtc tcggccctcg aacaccacca
    25981 tgatccgcgc gcccttttct ttgacccaat cctgaagata gcagagttcg gcttgaagcc
    26041 tgcgtagctc cttctcgtat gtcttgcgtt tgagtttcga ttcgtctttt tctttctgtt
    26101 tcttaggcat agctcttgac cctccgtatg ttattcatat gcgtcacacg ccctgtctag
    26161 accgtttttc gattcactct gtcatctcgt tccaggggaa ccgtatgggc gcccttattt
    26221 cgaattctgc ggcggccatt gttacttcac cgggcagggt ttgacattcc agatatcggt
    26281 tgcgtactcc gcaatagtcc ggtcactgga gaactttcca gaacttgcga tgttcattat
    26341 cgccttgcgt gtccatccat ccgaatccgc atacaggtcg cagacccgct ggtcagcctc
    26401 gagataggat gtgaggtccg ccaggtgcat gtagtgatcc ccgcctgcca gcaacgtatg
    26461 aagcagcggc gcgaagacgc cgggatcata acgactgaag tggtcgctgg caatcagatc
    26521 cagtgccctc ttggtctcgg cctcgtgttc atagtgccag tgcgggttgt accagcctcg
    26581 gctgccggcc acctggtccg ctgtcaaacc gaacagaaag aaattctcct cgcccgcttc
    26641 ttccgccatc tcaatggtcg cgccgtctcg tgtgcccaac gtcagcccgc cgttcatcat
    26701 gaatttcata ttgctcgtgc cgctggcctc gtagccggcg gtggaaatct ggttggagac
    26761 atcggtagcc gggatcagcc gctccgcgag cgacacgcag tattccggaa ggaacacgac
    26821 cttgaggcga tccttcaccg ccggatcacc atcgatggtc ccggccagat tgttcagaaa
    26881 cttaatgatg actttggcca gatggtaggc cggtgccgct ttgccggcaa agaaaaatgt
    26941 ccgcggggcc atctgaagtt tcggattctc ccgcagccgg ttgtacagta tgacgatccg
    27001 tagtgcgttg agaagttgcc gtttatattc atggatgcgt ttgatctgac aatcgaagat
    27061 ggagtcagga tcaacggtct ggccgctctc cgacttgagc cacttggcga acgtggattt
    27121 ggcgtcgcgc ttcgctttcc gggccgcatc gcgaaacccc ttgtcgccgg cgagtttctt
    27181 gagctttgtc agctgggcga gatcggtgat ccagccgtcg ccgatggcct ccgtgatggc
    27241 gtgggaaagc gaagggttcg ccatcagtaa ccatcgtctc ggtgtgaccc cgttggtctt
    27301 gttgttgaac cgttcgggat acatttcggc cagatctttg accgtcatgg agcgtaaaag
    27361 gcctgaatga atggctgcca cgccattggt gctatgcgat ccgacgatgg ccagattggc
    27421 catgcgaact ttacgtatcg ccccttcctc gatgaggctc acattggaga gccggctttc
    27481 gtcaccgccg aagcgcgccc gcgcgcggtc aagatgccgg cggttgattt cgtggataat
    27541 ttccaaatgc cgcggcaaca agagttcaaa ccattgatgc ggccattttt ccaatgcttc
    27601 gggcagcagg gtatggttgg tataggccag tgaacgttgc gtcagatccc aggcctggtc
    27661 ccagtcctgt tgggcctcat cgaggagaat gcgcatgagt tcggggacgg ccatcgtcgg
    27721 gtgcgtgtcg ttcaactgaa tggcgacttt ttccggaaga gcgttccaat cggtattgcc
    27781 gcgtcggaac cgccggacca gatcgcccag cgagcaggcg accaggaaat attcctgaag
    27841 aaaccgcaac ccctgacccc ggatggtgga gtcgtcggga tacagcacac gggtcagcga
    27901 ctcggccgcg agcgtaccgg ccaaggcggc cacgaaatca ccgctgctga accgctgaaa
    27961 gtcgaaataa tccggaaccg tcgcagccca gagtcggagg gtattgatcg tttttccccc
    28021 gtacccgatg accggacggt cgaaggggat gccgatgatg ctggtaggcc gcccggggat
    28081 catgcgtaaa atcccgtcgc gcagttcgaa cgaacagttt aatttcactt ctaccgactc
    28141 ttcaagccgt ggcacttccc agggatcggg acgccgcaac caattatccg gttgctcgca
    28201 ttgccagcca tccttgatcg tttgcttgaa gattccatat tcataacgga ggccgtagcc
    28261 cattccggga atctgcatcg tcgccatgga atcgatgaag caggcagcga gccggccgag
    28321 gcctccgttc cctaacccgg catccggctc gatctcggcg atggcgagag gatcaaggcc
    28381 ccgttttttc ccggcctcta acgccaatgg tcccagcagt gcgttggtaa cgttattggc
    28441 gagtgaccgc ccaaggagga actccatgga caggtaatac acccgcttgg gatttttttt
    28501 ctcgtaggtt gtttcggtct tgagccatcg ttgcgagatc acatcgcgaa cggagcgggc
    28561 cgtcgcctca aactgttcgc gggggtcggc cttcttgggg tcgatgacgt ggtcgaagat
    28621 caagtgtcgt tcataaagcg cgtcgttcgt gccggagaat tgaatgggtc cgcagccgta
    28681 ctctgcaaga atttccttga gttcttcttt catatcacct ccacgcaaaa acgtatgaga
    28741 tcagctagtc tgtgttctgg gggcaggatt gcgcccaggt ccgggtgccg gtccgccgga
    28801 tcttttcccg gatcggcagc gtttgacgtt tcatcgtcca ggtgacattg tttgagtcct
    28861 gctggtgttc aggtgcgcga acagcaccac gacggaccgt gcctcggtcc ggtaccgccg
    28921 accgggaatc gccggggagg attcccatgc ttcaatgtca tgcggcgaat ccagggcggt
    28981 atcgatccat ctgtaccaga tgtcgtcctc gtcgtggtgt gcggtgggca gttcgaattc
    29041 cagcggctcc caaaaggcat tcagcataaa gtggacgagg agcatttctt tgggaagttc
    29101 aacggtaaaa gcgatgctgt gtgaattcgg gccccagtca ggttggttga gtttgacacc
    29161 gtgccaggcc ttattcgcct gtcgaatcaa ctggttgaga ctcatccgtt gctgctctcg
    29221 ttcaacgtcg cgcaggagac gccgtgcatt cagcaacgtg acaaatcgat gaacgtcggc
    29281 gtgcctatga acaagcgtcc aatcgaacca attggtttcg tcgtcctggc agtatgtatt
    29341 gttgtttccg ttttgtgtgc ggcggacctc atcccccatc aaaatcatgg gaatccccac
    29401 ggacaggagg gtcatggcca aaaaattctt cacctgccgg ttccgtaacc gttccacgac
    29461 ctcattatct gtcggccctt cgattccaca gttccagctt ttattgtcgt tggccccgtc
    29521 gcggttatct tcgccgttag cctcgttgtg cttgtcgttg taggagacga gatcattgag
    29581 cgtgaagccg tcatggcagg tgacaaaatt aacgctctgt tccggctccc gttctttatg
    29641 cccgtagatc tccggactgc cgatcaagcg atcggcgaag cgtgccgacg aaccttcttc
    29701 gccacggaag aaatcccgga tatcgtcacg aaaccgtcca ttccattctt tccagctgtc
    29761 tccgataaac gagccgacct ggtacagccc ggcggcatcc caggcttccg cgatgagttt
    29821 cgtcccggcc agggaggggt ccgattcaat gtcccagaga atggggggat tctgcagggg
    29881 ctggccggct tcgtcgcggg agaggatcga tgcgaggtcg aaccgaaagc cgtccacgtg
    29941 catctcctcc acccaatacc ggagactatc catgattaaa cgacggacaa tcggctgatt
    30001 ggcatttaag gtgttaccgg tgccggagta gttgctgtag caggtccggt ccggctccag
    30061 aatgtaatac gcgccgtttt cgagaccgcg ataacagacc gtggggccat cctcatttcc
    30121 ctcggcagtg tggttgtaga cgacatcgag gatgacctcg atccctgctc gatgcaacgc
    30181 cttgaccatg tcacggaatt cgtccacgac gcccgtcgga tcctggcgcg aactgtacat
    30241 cggatgcggt gcgaagaacg aaaccggcgc atagccccaa tagttgactc gtcccgaagg
    30301 acaatcctgt gggtcgaaat ggaacaccgg caagagttca accgcggtga tgccgagtgc
    30361 ctgtagatac ggaatttttt cgatcagacc ggcatacgtg ccccgggtgt tttccgcgac
    30421 gcccgaactg ggatgtcggg taaacccgcg cacatgcatc tcatacacga tggtccgggt
    30481 agagggttgt ctgagggggg cgtcgccttc ccaatcgtag gcctgcatat ctacaaccac
    30541 gcttttcatg gctgttgcac aattgtctcc cgggtgggat gctgcgacgc ggctgtattg
    30601 cgtcggcatc gccacggctc tgccataggg atcgagtagg atcttctcag agtcaaatcg
    30661 tagtcctcgc tccggttcga aggggccgtg ggcacgatag ccataaatct ggccggggcg
    30721 aatcccaggg aggaaaacat gccaatagtg gtatgtccgg tttttccagg agtcgagggg
    30781 aataatggtc accggcttct tatcatccgc gtgatcgaac agcagcaatt ccatgagggt
    30841 gcagtttttt gaaaagacgc agaagttgac cccctcagga gaaacggttg ccccgagggg
    30901 aaaacttttt ccgggagcgg ttgttccggc ttgcatactc acttcctgtt tggcccccct
    30961 tttctgattc ttcggcatgg gtttcgatcc tcaggcagag atccacatgc cccaccctga
    31021 acctgtgact ggtctggtgc cagagtgggt ctctgtcttt tctctccctc ggcgttgctc
    31081 ctcagggttt gatgattaat ggttatttgt ttgccagttt ggccaagtct tctttgtata
    31141 ccggcgtatc cgattcgtac ccatcgggga gtgtatggcc tatgccttta tggcaatcga
    31201 tgcaggtttt gccttcactc atggctttct gcatgtcccg ttgggccacc agcgactggg
    31261 cgtgccagtc catggcctca taggaatggc aattacggca ttcctgtgaa tcgttttcct
    31321 tcatctcgag ccaaaccttt aaggccatct gttcacgctt ggcctcaaat ttttctcgcg
    31381 tattaatgga gccggtgatt ttaccccata attcccatgt ggccttaatc ttccgaatga
    31441 cctttggtcc ccactgcttg ggcacatgac agtcggcgca aatggcgcgc actccctggg
    31501 cgttggtgta gtgaatggat tgctgatatt ccggcaggac gaaatccgtc atttcatggc
    31561 aggcattggc gcaaaagggg agcgtattgg actgctccat ggcggtgttg aagccccccc
    31621 agaaaataat cccggctatt ccgccaacga cgaaaatcgc tcccaaggag tatttgctgg
    31681 aggggctcca aaaccagttc cagagtttct taagcattga atgatgtgaa ccctcaattc
    31741 tgtttcgtgt ttacttcaat gacccttttc cgtaacggga ttcaaatcct tttcgtacgg
    31801 cgtcaagctg gtcagggttc atcccttcaa agtaccaggc atggcctggg ataggtttga
    31861 aatgcttttc gagtaatgcc tgcgcttctt tttcacccgc gacctttttg agttccggaa
    31921 tgaattcgaa ataggtgtgc ttggccacct catacatccc atgccaccac gtgtaatcgg
    31981 gaccgctcat ggaggcaccg tgccgcgctc gacgaccttc gtgatgccag atttcccacc
    32041 aaatccattc aatcttctcg tcgaaggggg cttggctgat tgtcccggct ttctgtaggt
    32101 cacccatgat ggcggagatg ggcttagcaa atttctcgtt atagaggtct accaccttat
    32161 caaactggtt gtaatgtccc aatatcaccg aatcggtgtg acaggccgtg cacacatttt
    32221 gcattttgag acgacgttct ttccacgtca gcacttcaac gacttctgac ttcttggcct
    32281 tgtcgccgac tttgggcaat ggttgccctt ccggcacatc aaactcactg ccgtctttca
    32341 gtttcaccag attgtttttg acggaaatgg gtggccgcaa ggtccaggag atccgttcgc
    32401 ccacattatg ggtgggcttt tccccccctc cggcgctcat atggcaagtg gcgcaggtgg
    32461 gtgctgccga ataatccacg ccggcttccc atttatccga ttcgagattc atctcagtcg
    32521 ttttggcgtg atagatgatc ccatgcttgg attcgttata gacctcgatt tgaggatgat
    32581 ccggaccgac atgacatttt ccgcaggtat ccggcgttcg tgcctgcgct ttggagaagc
    32641 ggtgccgacc atgacaggcg gaacaggaac ccctagaccc gtcgggattg atgcgtccta
    32701 ttccggtgtt gggccaagtg gtgggtaggg gttttccatc ttcaccaatt tcaatggttg
    32761 agccgtgaca ttgtttacat ccggcattca cagcttccgg gccgccaacg acctcaccca
    32821 aaagattatc gaatgatgcc agaatatcgc cggctttcga atgatgcgac ccatccatct
    32881 cctggaattc cgtggtatga cagcgtgcac aatccttggg cgtcacaata atggagatca
    32941 attgcccttt atgcctgaac ccgtccttgt ctttcccatc cgctttgtga caatcaaggc
    33001 aattcacccc tcgttgcccg tgcatgcttt gattccattc ctggtacaac cctggtgaat
    33061 gttttcgatg acattgcacg cattcctcac cgtcgggatt tccccaatcc gtgccatagg
    33121 tgccggcggg tctcgccgca ttggcttcac cggtttctcg aagcagcagt gtggcaccgt
    33181 ctaacaacac cattgccgaa cttccgatca ggagaatcag aatgacacaa aacgatatcc
    33241 gaagagaatt tcctagccgg gaaccgtcat cgatgttctt cataagtgtt cctcacagaa
    33301 cgttgggttg acgattgtgg gttctcctct gggggttgag ggcccctcac cttgattggt
    33361 cccgaagttc atgtcacttt cttgagaaac gtatttttgt catcaatcat acgttgtcgg
    33421 cttccgtgga gctttttctt cgcccggtgg tcccgcgctg accccattgc caaccactta
    33481 tttccggcat gtcgtcgcca tatttggcaa tgtactgttt gtgctcgatg agcttgtcgt
    33541 gaatggcctg cttggcgtat gccgcccgag tttcgagttt cggcacgcgg tcgatcacat
    33601 cgtccaccaa atgaaagcga tccagatcat tcatcacaca aatgtcgaag ggcgtggtgg
    33661 tcgtgccctc ttccttatag ccacgcacat gcaggttttt gtgattggtt cggcgatagg
    33721 tgagccggtg aataagccat ggatatccgt gaaacgcaaa gataatcggt ttgtctttcg
    33781 tgaacagcgc gtcgaagtcc ttgtcgctca atccatgcgg gtgttcgatc tgaggttgca
    33841 gcttcatgag gttcaccaca ttgatgacac gaacctttaa ttctggaacg tgagtacgaa
    33901 ggaggtctac ggcggctagc gtttccagcg tcgggacatc cccacaacaa gccatcacca
    33961 cgtcgggttc gcctccacga tcattactcg cccactccca aatgctgaga ccagcggagc
    34021 aatgtttaat ggcctgatcc atggtcagcc actgtggagc cggttgcttc ccggccacca
    34081 cgacgttgac atagttccgg ctccgcaggc agtggtcggt cacggagagc aggcagttcg
    34141 catccggcgg cagatagacc cgaaccacgt ctgctttctt attgatgacg tgatcgataa
    34201 acccaggatc ctgatgactg aacccgttgt ggtcctgacg ccacacgtgg gatgagagga
    34261 gataattcag cgacgcgatt ggtcgccgcc aggggatttc gtcgcatact ttcagccact
    34321 tggcgtgttg attgaacatg gaatcgatga tatggatgaa tgcctcgtaa caggagaaga
    34381 agccatgccg gcccgtgagc agatagccct ccagccatcc ctggcactgg tgttcgctga
    34441 gcatttccat gacccgacca tcgggagcca agtggtcgtc gtaaggaaag gtgtcggcca
    34501 tccaggtacg gttggtcacc tccaatacgt cttgccagcg attcgagttg ctctcgtcag
    34561 gactgaacag tcgaaagttt ttgctgctca ggttctgctt catgacatct cgcaggaatg
    34621 ttcccatcac ccgggtggac tcggcggtga ctgatccagg ggatgtcacc tttaccgcat
    34681 agctccggaa atctggcatg cgcaggtcct taagcagcaa cccgccattt gcatgcggat
    34741 tggcgctcat tcgtcgctcc cctttgggtg gcagctctgc cagttcgggg ttcagctgtc
    34801 cattcttatc gaatagttca ttcggcttgt acgtcttcat ccatttttca agaattcgca
    34861 catgctcagg tttttcatgc atctgtccca tcgggacttg gtgagcgcgc cagtaccctt
    34921 cggttttttt gccgtcaatt tccttcgggc aggtccaacc ctttggggtg cggagaacga
    34981 tcatgggcca gcgtggtcgc ttcgtggctc cttttttttg cgcatccgcc ttgatgcttt
    35041 ggatttcttt ggtcacgatg tcgagcgtat tggccatgag ctgatgcatc ttcctggggt
    35101 catccccctc aacgaaatac ggcgtatacc catacccgcg gaacaagtga tccagctctt
    35161 cgtggctgat tctggccagg acgcaggggt tggcaatttt gtagccgttg aggtggagaa
    35221 tcggtaagac cacgccatcg gttaccggat tcaaaaattt gttggaatgc cagcttgtcg
    35281 ctagcggtcc ggtttcggcc tcgccgtcgc caacgacgca ggcgacgatc agatcgggat
    35341 tatcataggc agccccgtag gcatgggaga ccgcatagcc caactcacca ccttcatgga
    35401 tcgagccagg agtttcagga gcgacatggc taggaatacc gccgggaaag gaaaattggg
    35461 tgaagaggcg cttcaaccct tcttcatcct gtgagatgtt cggatatacc tcgctatacg
    35521 tcccctctag gtaagcattc gcaaccaggc caggcccacc atgtccaggt cccgtaatgt
    35581 aaatcatgtt caagtcgtgt tttttgatta accggttcag gtgcacatag aggaaattca
    35641 ggccaggggt tgtgccccag tggccgagaa gcctcggctt gatatggtcc ttggtcaagg
    35701 tcttcttcag taacgggttg tcatagagat agatttgacc gatagagagg taattcgccg
    35761 ctcgccaata agcatcgatt agttccagtt cttttttaga taagggattc gccatgctga
    35821 cctccttaca ggtacatcaa taaaagggga ataaaactga aagtttcaat cgatgatgaa
    35881 atgaatcgga tgccgccatg ctcagtggtg tcctcaatac ttacatggtt gcctgaagac
    35941 agcgcatggt ttcccgtgca atccaggttt cctcatcggc tgctcccaca tacgcctcta
    36001 agagatgccc attctgactg atccgggcga cgtcacccgg ctgaagtcct ttagctgcgt
    36061 gattgaggtc tgagtccaat cgcaatccgc accattccat tccctcgcaa atcctggccc
    36121 ggatggtggg agcggcctcg ccgattccac caccaaatac gatggcgtcc gcaccgccca
    36181 gaaccgcaag ataggacccg atgtattttt tcgcgcgata gcaaaacaca tcaatggcca
    36241 tttgcgatgg ttcatgccct tcgcggtcgg ccgcctgcag caattccctc atgtcgttcg
    36301 tccttccgga cagtcccagc aatccggact gttcattcaa ccaccggtcc acctccgagg
    36361 aatcgacttt ttctttttga cagagatatt tcacgatgga ggggtcgaga tctccggatc
    36421 tggttcccat caccagacct tccagaggcg taaatcccat cgacgtctct atagatttcc
    36481 cgcccttaat tgcggaaatg gaacatccgt tgccgagttg tagggtgatc agccgggatg
    36541 tgccggaagg tctcccagtt ttttctatat aattctcgaa gagagaggca tgggcgattc
    36601 cgtggaaccc gtaccggcga atgtgatgcc gatcggccaa cttatgggga agcgcataca
    36661 cttttgcatg agggggcatc gtatggtgaa aagccgtatc gaacaccgcc accatcggca
    36721 ccttgggacc cagcacgtcc ctcgctccac gaatcccttc caagcacccc gggttatgca
    36781 ggggcgccaa ttccccgagc gcatcgatct cgcccatcac cgtatccgta atcatgaccg
    36841 actccgaaaa tcgttctccg ccgtggacaa ctcggtgccc gacagcttgg actccggtaa
    36901 gggacccggc attcttgctc ccgcggtcaa gttggtcaag ttgctcaaac agccagcgga
    36961 tagcctggtg gtgatcggcg atcagccgag tcattttctt gtgaggtgtt ccgtccgtct
    37021 ccagttccaa gctcgccgtg ctgccgatgt cttttaccgc gcctctgata ggggtccgag
    37081 ccggtcgaac accggaagtc gatccttccg ggaccaactc gagtacccga aactttacgg
    37141 atgaactccc gctgtttaag accagaactc gcatgggtcc tttcgggtca acgtgactgc
    37201 caggtcacga tcggtcgaat ggcaacggga gtctcgggag aaaggttgat ctcttcctcc
    37261 tcatgccgga tcatgaaacc cttgccctcg cgcaacgaat atttcgcgcc ttcgggcccc
    37321 atatcgatat caaggatttg gccgcggtac gtcaacggaa agcggatcct tgccggctgg
    37381 gcagggaggc gtcggggcct gaacgtcagg ataccgtcga agtcccgcat ccccgccatg
    37441 ccatagacca ggaccatcca ggtgccgccc atggaggcga tgtgacatcc gtctttcaca
    37501 tttcctccga tatccgccag gtccatgagc aaggctgccc gtgcatattc ggttgccaaa
    37561 tccgggtatc cgatttcgga ggccactata ctctgaatgc aggatgagag agaggagtcc
    37621 cctgtcgtca gggggtcata ataatcaaag ttgcgcttct tttgctcgag ggagaacgcc
    37681 tgtccgaaca gaaacatggc taaaataatg tcggcctgtt taataacctg ataccgatag
    37741 atgaccaagg ggtgataaaa caaaagaaga gggtaatttt ccggtgggat attcttcaga
    37801 tcccaccgct ctttttgtaa aaaggcatca tcctgcgggt gaatttcccg ctcttcattg
    37861 taggggatgt gcatgttttc cccagccctc cgccaatcct tgacttcctc catttctatc
    37921 cctgttttac tggtcagggc ggcgaacgca tcggggttct tttctctcag agactcgatc
    37981 gtggacgcgg catacagaag attttcccgc gccatgagat tcgtgtaggt attattgttg
    38041 acgacggtat tatattcatc agggccggtc accccatgaa tgcaaaactt tcccttcttg
    38101 tcgtcggaga aaaatccgag gtcgctccat aggcgggccg tttcgatcaa catttccgcc
    38161 ccatagtcat tcagaaattc ctcgtcaccg gttgcctgga cgtatttttt tagggcatac
    38221 atgatgtccg cattgatgtg gtattgagca gtccctgcgg cataataggc cgaggcttct
    38281 tctccattga tggttctcca gggaaacatg gcgcctttct gactcagttc ccgtgcccat
    38341 tttctggcct tgtccagcat gcgataacga aacatcagaa gatttttggc gacgcgtggg
    38401 gaagtgtaga tcaggaaggg cagcagatag atttccgtat cccaaaaata ttgaccttca
    38461 taggcctggc ccgtcaaacc cttggcgggt acaccggtgg tatctgctct gcccgacgcc
    38521 tgaaggacat gaaacagatt gaatcgtatg gcttgctgaa tttctatatt ggaacgcttg
    38581 gtccgggttg gatccagatt gctgacctgg atatcgctcc ttgaccaaaa atcagacatg
    38641 aattgttcct ggtcgtcgag gatgagagcg aatccctgtc cgagggctct atccaaagtc
    38701 cgttcggctc tggcacaaag tccttcggga gatgcggtgt gcgtcgtgtg ataggtcatg
    38761 tacttgaaca ggcggacggg gatgcccggc ttggcatcga tcgagaaaac gatggccccg
    38821 aagtcctcgt aacacttgtc ctgaatggtg tacgcacagt cggtcgccag atcgtgatcc
    38881 atcccgcatg ccagtgtcat gttgctgttc tcggttccgt gacacagaac aatgcgttga
    38941 tccttggcaa tctgctttct gggatgcaaa gtccggtcga gaaaaccctt cgcctgtcga
    39001 gggtcactgt ttcccttctg atggtcatga agatagagca tttcagagga cagcacgatc
    39061 ggcgcctctg cattcaacac cgtcacttca taggagatgg cccccaggtg gcggtgggag
    39121 aatgacacca ggcgtctgga tttgatgcgc acctgtttcc cggacggggt ctcccacaca
    39181 atctcccggt ccagtgtggc ggatttcatg ttcaggcggc ggtcatagtt cagcagattg
    39241 gcgttgggta aatacaaagg ctcgtcgtca acatacagtt tgattttgtt gctgtttgtc
    39301 acattgacga tggtctgacc ggttcgggca aagccatatg cctcttcgcc gtatacgatc
    39361 ggccatgatt cgtagaaacc attgacaaag gtcccgttct gaacggcggg cactccctct
    39421 tcaccgcatc ctcgcatgcc aaaatatccg tttcctatcg caaaaattga ttcggtcatt
    39481 tccaggaacg ggggatggaa ttgtttttca atcagattcc attcgtcggg aggataaata
    39541 tgagggggag gcttcacacg ttcatggtgt atcatgagtt ttcacccttc caatgaacag
    39601 gcgatacgtg aagaccccgg cacgccagcg gaaaaggcga tatgtcgagc tgatcgcccg
    39661 tccaaaaaag agaaggaatt ctttcgggac gtttagacaa gcaattctcc aagatcgttg
    39721 actaccatat cggcgccatg ttttctgagt tcttcggaat cttgttttcg tgccactccg
    39781 attaccaacc cgaatcctcc ggcccggccc gcttgaaccc cggaaattgc atcttccacc
    39841 accaccgcgc gtttcggatc cactcctaat ttttttgcgg cggccaggaa agcatccgga
    39901 gccggctttc ccgagagatt ctcctggtgg atgacgtttc catcgacttt caccttaaag
    39961 agatctccaa ttccggcagc gttgagtaca atatcgcaat tttggcttga ggtcacgacg
    40021 gcgacttcga ttccatcttt gcggattcgt cgaaccaatg cgatacttcc ttcgtaggcc
    40081 tcaacaccgt tcgtctcgat gaccgcgttg atcatttcgt tcttgtggtt tccaaggccg
    40141 catacggtct ctgtttgtgg tggatcggcc ggagtccctt cagggagttc aattcccctt
    40201 gactggagga aatcccgaac accatcaaat cgcggttttc catcgacata gagacgataa
    40261 tccgtggcga tctcaaacgg ttgaaacggt ccaccagtcc gggatgcccg attctgcagg
    40321 tattcgtcga acattttttt ccaacagatc gcgtgaactt ttgcggtcgc agtaaggact
    40381 ccatcaaggt caaacagcac ggcatcgaat ttgtccgggg ttatcgggta aagcgacttt
    40441 gctccgctca tgtttggtct tccctctctc tgaaggaaat gaatggagcc tctaaatcgc
    40501 actcacaatc gctttgtcta aaagtgggca tcgaatacgt gcctccatcc atctcacggg
    40561 ttaaaagtga ttcgcccaaa cacggcttag tcatcatgcc agtcggcccg gtgtgccggc
    40621 atcctttttc gggaagcatt tccttccatc tcttctgtgt atttcccgag gctggcagct
    40681 ccgttacatt tggctctctg ataaagcgcc tgttggccgg ccttgatatt gttattgtcc
    40741 ccatgccagg cctccagggc agcgtcctgc agagcccggc catacgaaaa gctgattttc
    40801 cagggcttcg gtgaaggaag gatatttatg gcattcaaat gtgctgtggc caggcggtca
    40861 tgttggccgc ctgatagaaa gacaattccc ggaacagccg ccggcacatg acggcaaagg
    40921 cattgcagtg tagcctgagc aaccttttgt accgaagcct gcttggtgca ttccttgcct
    40981 gaaacgacca tattgggctt gagaagcatc ccctccagcg aaaccccctg ggcataaagg
    41041 gcgttgaaaa ctgtatggag cacatgcccc gtaatctctc cacagcgttc gatggaatgg
    41101 ggcccatcca tcagcacctc gggctcgact atgggaacga gcccttgctc ctgacagagg
    41161 gccgcatagc gcccgagtgc gtgagcattg accgccaggc aggtttgact tggatgcctg
    41221 tctgaaatct gaataaccgc ccgccatttg gcgaatcggg cacccatgtt gtagtaggcg
    41281 gtcaatctgt ctcgaagtcc gtccaatccc tcagttattt tttcgcctgg agatcctgcc
    41341 aggtctttag cccctgtatc cactttaatc cctggaatga tacccagtct ggtgcagacc
    41401 tccggcaggg gaactccctg ggcgtttgtt tgtcgaatgg tttcgtcata cataattacg
    41461 ccgctgataa actccgaaac tcccggactg gtgaaaagca tttcccggta ggcacagcga
    41521 ctgttttcgg ttgagttaat tttcagggac tcaaatcgtt tcgtgattgt cccaaccgtt
    41581 tcatcggccg caagaatacc tttcccttct gctatcaggg ctttcgccgt tgcttggaga
    41641 ctgtcgtggt tcatggagtt atccccctac agttcgcgac ccgcaaaact aaaaatgggt
    41701 catgacggga acaggttgtt cagataacga taatttttag tcaataagaa ggaaaaaccg
    41761 gtttgagcac cgcgcccgaa tatcgggtaa gctgccccgg caaaccgtac ggtatcgatc
    41821 ccctggattt aacggtctta tcattctcat atcgatcgga ttggttctca gttgggtgaa
    41881 tcggtatgtg aggatgattc gaaaacgttt cgggccattc tgtaaagcgc aattgcagtt
    41941 cggcggattg gcggagggcg atgaccccta cttttgggtt caacgtgtca agaggaatct
    42001 tggatgaagg gcgtacggta aaattcttgt ctttggtatt ttccaacgga atgggcaggt
    42061 gaacgggatt gaaggcttct gctggtaggt caaaacacac ttgctccgtt tccaaaaaaa
    42121 tgaaaaatga acgctcgacc tgtacctcaa taaaagtaaa ttttgactca ataagcgggg
    42181 cgagcatttc taccagtttg taccaaaggt tttttgaaac gtgtatttaa ctagcatata
    42241 attgaaattg acatcaagta tcacatctgg atcgtgggat catcagtata attcaggcat
    42301 aagccggatc caaaacattc tatggtgttg ctagcttaac ccaaataatg actcaaaaaa
    42361 aacatatggg ggcagtgaat ttacaaatgc gtaacacata cttcatatat gaatatgtga
    42421 gggacggacc atggtctttt ccttcattcg ggagggatgt tttttgcctg aatcatggct
    42481 ttgcagggag tcgaacgttg acagtagggg tgaacgaatg gttacagttt cctcttccct
    42541 tgtgaatttc attttggatt gctgatggcc gaaaattttc aaaaagttta cgtcattcgt
    42601 catggagaga cggaatggag tttgagcgga caacataccg ggaccacgga tttgcctctc
    42661 accgaaaacg gccggaatct ggcacgactt ctccagcctc ttttttcgaa ttactccttt
    42721 gacctggtgc ttaccagtcc tctgcagcga gcccatgaaa cgtgtgtatt ggcaggattg
    42781 ggcgagaagg ccgaggtgga caccaatctt gtggagtgga attatggaga gtatgaaggg
    42841 gtcacttcca aggagattca agaaaagaga cccggttggc tgatatttac cgatggttgt
    42901 cctggtg
//
LOCUS       DAORAQ010000008        40564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_216_length_40564_cov_61.429534, whole genome shotgun sequence.
ACCESSION   DAORAQ010000008 DAORAQ010000000
VERSION     DAORAQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 40564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40564
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216_length_40564_cov_61.429534"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKM72_01630"
     CDS             <1..381
                     /locus_tag="PKM72_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59506.1"
                     /translation="LPTSGLQAGATVYIDRSYTFATVPTLVQGGAYIRTANDDKAATN
                     STFLSFTVNQPVSVYVAHGDRITSKPSWLSTFTDTGTNLVTSDATFSLFVKSFPAGTI
                     TLGGNVSNNNAGNDLSMYSVIVKQ"
     gene            1092..2633
                     /locus_tag="PKM72_01635"
     CDS             1092..2633
                     /locus_tag="PKM72_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59507.1"
                     /translation="MKSRYATNPAILCWSLLAAVFMLNATQSIAATYYISPTGSNVLG
                     NGTSQNPYYTLTHAFSKGGGHTYILKDGLYNYEGGTIVNPPSGSTTSPTIIQAEHDGK
                     AVIDGAGVRTGVDIRQAGTHHIVIEGLRLQNCGEGPSVSVQSPDGTSLTNQINNITIR
                     RTGAYGSAVNTNNNVWSLSRIRDSLFEDVWGWGAGRYVLSVYGGTKVTVRRAVLRWDR
                     WDGLAYKPGDPKFNMSLYNTYDSLVENVILLDGTTATGGGDRGGILLAGNSNGDTAPY
                     TGSSNNVILGMISLNNVGNGLASESGGGINNNNRFENVVSWGNEYAITVPRKSSNTTL
                     NHVTLGGGSYGTYFGGGGNQVTNVSLKNSLIHNNSNSGINGSVTSDYNNVYSNSPNYN
                     GASPGAHDSSIQPGLKYLLRAETGTPNKGAASDGGDIGATIIKRYQNGTLTNQNLWPF
                     PNEARIKADLCANESRGFCGKSSLTHYIWEFLGNPLPAEYANGGGTSDTIPPASPKGL
                     TLTIQ"
     gene            complement(2722..3924)
                     /locus_tag="PKM72_01640"
     CDS             complement(2722..3924)
                     /locus_tag="PKM72_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP59508.1"
                     /translation="MPMSLKVLFISQIFPTPVNPTKGVFSHQLVQDLTNYCEVRVVCP
                     QPWFPKLPRWKFLERWHGYSLIPIEYAIDSIPVLSPKYPFIPKWGDHYHSFLLFLGLV
                     RYAYALRRCFRFDVINAHWLYPDGVAAALLARIMGVPLVLTGLGCDVNMFLFEKRKRG
                     PILRALNQASRVTVVSEELKAHLLGVGIDHKRVTVIRNGVNKQLFFPRDQTDSETILG
                     LPQKGPCVLFVGRLAEEEEKGLRYLIEAFQELVQIRKPITLYIIGDGPMLKAYQELVH
                     KQDLQGHIQFVGAKAHQDIPLWIGACDVFCLPSLREGCPNVILEALSCGRPVVASRVG
                     DIPNLIGKTNGILVEPGDSHQLSEALDMALAREWDEGEICGSVGNLTWDAAASQYYSV
                     LLGACSDV"
     gene            complement(3958..>5055)
                     /locus_tag="PKM72_01645"
     CDS             complement(3958..>5055)
                     /locus_tag="PKM72_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNP59509.1"
                     /translation="PLGAFLSGGIDSSAVVAGMSKFMTEPVKTFSVGYQDDKDASELP
                     YAQCIANIFHTEHHEYILESEEFFASLDLLLEYTEEPIVESAAIALFHLSRLAKEHVT
                     VILSGEGSDEILAGYPLYPIMARLDRIHRVFGYLPSWIHECLSTSLKANEKYTKYVDW
                     MRMPLLNRYRGISNDVTPSIKRKMYQPGFASRLGNDSDVFFEKLLGTLSQSSGLRKMG
                     YLDIKSWLPDDLLVKADKMTMANSLELRVPFLDHHLLEFAMSLPDEYRLKGWEGKLAL
                     KKVMEKVLPHEIIYRKKKGFPVPVARWFKEKLYHQVRDVLLDSKSLTRNYFQESYIDN
                     ALKRHRQGKEDLSRRLFSLVALEMWHKKYID"
     assembly_gap    5056..5155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5156..5589)
                     /locus_tag="PKM72_01650"
     CDS             complement(<5156..5589)
                     /locus_tag="PKM72_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNP59510.1"
                     /translation="MAHRGPDSAGIFLNKHVGFGHRRLKIIDLHAGAQPMFNEDGTIA
                     VVYNGEIYNFLELREHLIAKGHTFQTKCDTEVIVHAYEEYGIDCPKWFDGMFAFALYD
                     VKRKSVFLARDRVGIKPLYFYVDQHQFLFASEVRAILKVLEFR"
     gene            complement(5695..7059)
                     /locus_tag="PKM72_01655"
     CDS             complement(5695..7059)
                     /locus_tag="PKM72_01655"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNP59511.1"
                     /translation="MVKEETADLEPVDSIGPNRASGNGPIPSPLSFLLFLYFITLYLR
                     PQDWVPFMLNWPVDYIIFAFLLGGSIFQMDGWQRLCKLPLLYVLVVWLVVISLSNLAQ
                     GGEGAWYARAAFTEYSKSFLIFMCLFLFLNSTGQIRRLIWFQIFLSGLLAYQCIDQVA
                     TGVGWAGQHLGWDDGHGGRAKWVGLWDGMNVLALLFVISFPFLIQFMFSPWNIFIRIT
                     SFLFSLLIFQGLLLTQSRGGLLATIVALFASLVLRFKPRTALILGAIVLVLAAPLVGF
                     LTTRGFDDSDGSKSAAHRVDLWAAGLEMAKENPVFGVGKMRFKNAARDKTGLSLIAHN
                     SYVENLGETGFPGLFIYISLSYLSIKGLWHVSKQVSDPKDRSLALALLASICSYAATS
                     MFVTTDFILFYVQLGIAAAFCSLNGFLPRLTLNDIFWIAVIEVGFVLFLRLFTALFFA
                     GGMS"
     gene            complement(7124..8299)
                     /locus_tag="PKM72_01660"
     CDS             complement(7124..8299)
                     /locus_tag="PKM72_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59512.1"
                     /translation="MYLVSGLLGGFLIILGFVGLVLRQKQMHLWIASYVIQQFRSRPK
                     VTAGPIHIFFCVTDHYEPAVGGVPYEQECARVEKWMKNYPHIASRHRDVSGRCPQHSF
                     FFPAEEYRPAHLDHLATLCGMGFGDVEIHLHHDNDTPEHLRDTLHEFKTILHERHDLL
                     RKNPESGEIEYGFIHGNWALDNSGGAGRWCGVNNEISILRETGCYADFTFPSAPDESQ
                     VATINSVYYATDDPLRPKSHNTGVAVEAGRKSSGDLMLIQGPLGLNWKSRKLGLLPRI
                     ENGELSGDNPPSKERVDLWIKTHIHVQGQPNWIFVKLHTHGADEQNMAVLLGEPMDRM
                     WTYLEDEYNDGQRYCLHYVTAYELFQAVKAAESGVALDPSFYLAKFGRSFQATTKNK"
     gene            complement(8327..9463)
                     /locus_tag="PKM72_01665"
     CDS             complement(8327..9463)
                     /locus_tag="PKM72_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNP59513.1"
                     /translation="MKLLFWAAFGAVLYTYFGYPLLVWVFARIRPRPIKQGAFYPSVS
                     MIIAAYNEEKVIGSKMANTMALAYPEGQLEIIVVADGSTDQTVSIAQSYEKQGVTVLH
                     RPARQGKTAALNHAVSVATGEIVLFSDANTVYEPTVIQKITRNFHDPSVGGVSGRKVI
                     LKDCDRQTSQGETAFWNYESWLKLGESRLGSIVGADGEIFAIRRALFAPMPSFIVHDD
                     MFLSLKIIEAGFRVIYEGEGTSAEYSSKTLHDEFFLKVRYASAGYQILTQFPKMFFPP
                     KTFFAFQFISHKLFRWLIPFFLLTMLLSSALIPSLWYHSLFWGQVSFYLAAVLGWVLM
                     KTLGRATFFYVPLYFCMGNAAALYGFFLHFFSAGQTSLWRKAER"
     gene            complement(9661..10851)
                     /locus_tag="PKM72_01670"
     CDS             complement(9661..10851)
                     /locus_tag="PKM72_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP59514.1"
                     /translation="MKFLYHHRTLGRGGEGVHIASMVRALETLGHTIQVASPSGVDPL
                     RMAGASPVDKGQAQVRGITRIWKFVSCWVPDIFFEILEIGYNVVVMIRLGPILLRRGQ
                     DVYYERYAFFMFMGVFLAKFFGWPVLLEVNEVVGVQRARNQVLVPVSQWFEKMIFKKA
                     DVILTVSSFLKEEVLKRGGKPGAVHVIPNAVDAQVIETTEWGDQIRKRYHLNGHTVVG
                     FAGWFDTWDRLDLLIKAVGDVHKEYPHIRLMLVGNGPVVDGLIEQIRASKLEEVVFLT
                     GPVPRSQVLSYIAAMDICVLPDSNVFGSPIVLFEFMGMGKAVIAPDLLPIRDIIEDRK
                     DGLIVQCGDPGALRQALSLLLGDSALRMELGARARKKVLGQHTWHENGKKVERFASAL
                     IDQS"
     gene            complement(10864..12630)
                     /locus_tag="PKM72_01675"
     CDS             complement(10864..12630)
                     /locus_tag="PKM72_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP59515.1"
                     /translation="MANQTKVAHVVLSLQPGGLERLVCTLVCSPILSTLPTIVLCLDE
                     PGVLAPMVENAGCRIVVVKRRPGLDLGLIGRLVKVLKREGVTVVHTHSLDPMLYAGWA
                     AWWMSVAVRIHTQHDIWLRTYNWKDRLKFRVGSLFFHKIVGVSQDTTRAFGEYGISSP
                     QGLTILNGIEEKKFSNPHNGKNMAFREAPDFQNQREWVVGTVARLSPEKGIHHLLHAY
                     SVLQARGIALRLILVGDGPQRGELEALAEGLGISSSVEFMGYQENVEIFLSTFDFFVL
                     PSLTEGIPLSLLEAMANGLPVVATNVGGIPEIIVHQESGLLIPPGNPEDIAKALSRLI
                     QDPGEAKRMANNAQDRIRDHFGMTPMTEAYQYLYEMEPPPSFLKNIVKKVAQKFPRRW
                     MLWNGERDTRHIAITVDDGPDPTYTPQILAILKTYGVTATFFLVGEKIERHKELVLRM
                     IEEGHELGNHSFTHPSFDHLSWKEVVVEIERTQALFQSITGRPCRLFRPPYGKLCLGS
                     IGGAWLARLTCVMWNVDLKDFQAESPGEILAALQCQSLQSGDILLYHGTNPSAVKALP
                     NILDVIVRQNLNPVHISKMLTV"
     gene            complement(12689..14170)
                     /locus_tag="PKM72_01680"
     CDS             complement(12689..14170)
                     /locus_tag="PKM72_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNP59516.1"
                     /translation="MNMQSDMKRLFKHSSIYAVGGIVNRAGAFLLLPLYTSYLTPSEY
                     GTLELVYGVTALVSSLLGVGLAHATLRFYFEFQEELERKFLVSTTLVGSSVIASSGVA
                     LLWFTTPILSGFLLGSRQDASLLQLGLLILILQLSTEIGLAYFRAREYSTRFVVASFL
                     RLLLQVGCNYYSVAILGWGVWGILLGNLMSVGISWVYVVGTTVYECGLRFHQAKFRQV
                     LTYSFPFLLSTIVGVAVLYSDRFLLKALYGLEAVGLYALALKFVELGNVLVLEPFKNS
                     YGSFRFSIMKNENVKELQAKIVIYMALVVGLLALGISAFSPAILRMLTTLEYWPAEHL
                     IPILAAGLLVSSLGYPFQTGILYLKKTKYLFYVTVLAGFVKIILSVAMIPRLGAMGAG
                     FAYLLAEVVSVSLMLFYSQQLFPIQYDYRALGKIGGICALLLGLNWGILFMGYGGFLG
                     AGIAIVSCYPFLILSAGCLNWSEVEAGWHFAVEEIRRLRGTTI"
     gene            complement(14243..15958)
                     /locus_tag="PKM72_01685"
     CDS             complement(14243..15958)
                     /locus_tag="PKM72_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNP59517.1"
                     /translation="MASTIHERLADLVEGYGWLDGVGKILNELGSEERKKLFWEIHLG
                     SQAAWMLWVGRTSQDTVLVLDAGLGTTTVALSYLFINVTGVYRDEPSRRCAIARARQE
                     GRKIKTVLYGLNQSLSFQEGLFDLVCLPDPQVWLEAGVGTVRKARDFFSELFNLLTPG
                     GMIYLGMREGPLFSDIGYEATRVYQRGLGSAMVRNSSRVLNRLSFYPTCGPVEAVFEE
                     RNKKSWVSKGRSTLKKVLRGESYGLIVGKSCPERSSGVIGDIVSQPEIDQSDRSDIQV
                     YVGTANTLVGKLPGRIVRFPLGDEPLFRCRNNYETLHALQGKAPVAIPAPYNLRRFGD
                     LRYFVESKLPGLEPTGTRKCSWESDRMTAQALHFILQLHTATAQKVILDDALFERLVG
                     ASLCHLMGYSNGTDKEMVAKVEALLKEGLLGKEAVLVRTHGDFKRTNLLVNESGEVVG
                     LIDWDLSREMGFPLMDLLWYLSYEVYLKQRIPFYQAIVRVAFEEDLFKNECVQTYWHA
                     LDLGPGDRQKIYAVILLLYQFHEHFDHWHKSEEDWFSHAMMPILRSAFEKALATVTEA
                     NCSVS"
     gene            complement(15958..16941)
                     /locus_tag="PKM72_01690"
     CDS             complement(15958..16941)
                     /locus_tag="PKM72_01690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNP59518.1"
                     /translation="MGHKLNFTEQSIVRLPLVSVVVPVYNAAKFLTETLNSILCQTYK
                     QIEVIAVNDGSTDSSRDILKEFSGRIHWIDQENYGVAIARNVGIAHSRGNFVAFCDAD
                     DIWFPEKIECQMDLMHRHPDVGVVGGLLENIDELGRKIEPCLCYLDLYNKPINLRQIL
                     LMEGNVNGIDMSTAIIRKNVLEKIQGFDPEHKHLKAEDYDFWIRISNQTKFYLPYEYF
                     GQYRVLRKSRSHGSLRKEYSAQFQILQMYRNEYSLENYNIRKAKIYSEWAESSFVVGE
                     KHAWKLQAKAMKLHPANLYHQVRFGWGLIKHMVKSLVRPFQGNIGFRKDIG"
     gene            complement(16944..18971)
                     /locus_tag="PKM72_01695"
     CDS             complement(16944..18971)
                     /locus_tag="PKM72_01695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNP59519.1"
                     /translation="MNSPLASSPGVSISKSELGCAIVCLAAFFIGVLARFDALGTWCL
                     AVDEFYIAKSVELIQQKGIPLYPHGGMYWRGLLFQYFEAASAGLFGLNEYALRLPAVL
                     FNLLTIPPLYLLGRKRIGNAGALLLVAFFCVSIWEVEFARFARMYSMFQCAFLWFLYY
                     FHEGFFLGRKRAILWSYAIASISTVIYEGSIFMACLLFLPLLIRGVSLVNQLRYGIWA
                     SAILLLNVVFPKVWLWLFPGGSENEYPKGFVLPQEGGGPLLQPDISLWESVFLRGDLW
                     GVGYMVLVLMGIGLAYMGMKNALWDWRQKTIAVALLGAGLFQLFGVVLAILLVSYFTQ
                     TIDPQSVLRRSIERSIAIFLVACLGFWLTVGFLMTTGYMVTQGPESLSLHKIFAIVFH
                     YPRVMEEFVFLWLDVMPYFLMVSCLFGFMVLWGSTESRQFLNNHFLLLVLVSCVLILG
                     VINTMYNSTRYSFFLYPLVLLLWFEGGLVLKSWLDSKFFPNTNLGRIPKCAVVFLPFG
                     LLLCSEDFSFAHIMNVGKAEVNFRMNVPEKVSVHYYQRADVRGPADFINEGWKPGDLI
                     LNAVPSSSFYLTPENYIFYDDQSSLFWENSQDRGRKDRWGNLILHNKETVQKAVQEIN
                     GTVWILTKPRYRSLLIGWVTEVFGEKNSVIQSGVAGKDHRIQVVRVVRYPS"
     gene            complement(19057..20046)
                     /locus_tag="PKM72_01700"
     CDS             complement(19057..20046)
                     /locus_tag="PKM72_01700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP59520.1"
                     /translation="MTVHSVHPACVTSEPLQVAVSVVVPVTERCDALDEVYRLHADIL
                     RRMVPSFEFIFVLDEGFEQQAESLNALIAKGEPIRMVPLQRHFGEATVLMVGFQEAKA
                     EVIITLSAYFQTLPEGLHTVMDSLKEGYDLVATRRYPRKDPWINRLQNRIFHFAMGLL
                     TGVEFHDMSCSLRGIRRPVVREVNLYGDLHRFLPLLAYQRGFRVVERDVAQHPADCHS
                     RMLRPGVYLRRFLDIITVAFLFKFTKKPLRFFGLIGAGLFSGGLVISMILAIQKLFGL
                     TGLTDRPLLILGVLLMVLGVQTASVGLLGEMIVFTHARKMKDYTIKDLLKAKP"
     gene            complement(20270..21193)
                     /locus_tag="PKM72_01705"
     CDS             complement(20270..21193)
                     /locus_tag="PKM72_01705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNP59521.1"
                     /translation="MMRPLLSIVVPIYNEEECVHPLYEAIRTACERNEQSYEIVFADD
                     GSKDRTYELLEDIHRKNPEVTVIRLRKNFGQTAAMAAGFRAARGHIIISMDGDLQNDP
                     SDIPQLLAKIAEGYDVVCGWRKERKDKFLSRRFPSLIANWLIGKITGVAIHDNGCSLK
                     AYRASIIKRVALYSELHRFIPAMATLSGARITELVVTHHPRMFGKSKYGISRAWRVFL
                     DLFLVKMVTGFAARPALWFGILAIPGALLAGLSLVGMVFSETIGIVFPATTFLLCALT
                     GHLLTMGMLGEMILFSGDFRPDQMIWRDQTQ"
     gene            complement(22157..23227)
                     /locus_tag="PKM72_01710"
     CDS             complement(22157..23227)
                     /locus_tag="PKM72_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59522.1"
                     /translation="MRILFLGIISLAMLGQAFALDTIPEKLSDWTEKGVVLQNSSGVA
                     WENKPGIAVSGISKVGGIYYLHYLAGFNGCWDADGDVNHQSLGLATSTDGVNFTKFTG
                     NPLLKPHDFVSVSSQEEGIRTAYIQYLPSKGKFYGYFGVESPGGSQTCNFGGGGTCGC
                     NISVDSRVYGATSVDGKAWTVEGSVGGTYSSSGSEVYAAGWVYDGSQFGLYVTTAEGG
                     QLKSASRGSNAWSLNELGGISALDWGWSGLDVYLHDDNNTLTLIYNPQGGSHPGSSND
                     NLYFATTHLDDMRNIQNERVITTSGVLQNKILRDGNEWKWYYSDDPSWNNNVIRLRTH
                     PIANSDNVPPEPPKNLNVAPIP"
     assembly_gap    23959..24058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24088..25095)
                     /locus_tag="PKM72_01715"
     CDS             complement(24088..25095)
                     /locus_tag="PKM72_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP59523.1"
                     /translation="MIDGMVSRITGLAKKVIMSNKALDRLFYDFSNMGEFSSLYQHEK
                     MLADHLRVDTYHDAIRAHVKAGDTVIDLGTGTGILALFAARQGAKVYAIDHSSFIDIA
                     RTTAAKNGIDTITFVRSNSQRFNPPEKVDFIIHEQMGDDLFNENMIHNLLDLKRRALK
                     STGKILPGRFELFLEPVALKKGRRMPFLWEKPVTVRDLPQGVDFSFLRDLPEAKAFQP
                     PGYIRHMVEEAAVDFFLCEPQPIMTFDLNKMDDASTVPMHWQANKRVNRAGGLDGFCL
                     YFLADFGEGFQLDTRPGRTHWPNRLFRMPRVEYAVGDTIRYAVHVGDIRRSYTWTFTV
                     E"
     gene            26752..27624
                     /locus_tag="PKM72_01720"
     CDS             26752..27624
                     /locus_tag="PKM72_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HNP59524.1"
                     /translation="MSLKVVIISYCGASEAYVARKIIEQYPSTVIIQPVRSQKAPEVH
                     ISSLTGATRLLRNWLGKMGERRHRDRLINRLYPGGTQPSIPNIVPFPESDMNSRPGVE
                     LIDSLQPDVLMSCGAPMLSAELIGIPKLAAINIHYGIPPHYRGNNTLFWPMLKRDFDN
                     LGACIHHLTKGVDTGNLLAVIRPALQPKDGLLSVEFKITHLLPGVLLEFLALIEQSHA
                     VPGIPQTGAGRNYKKKERTIRKRIEYHSKRTFGLLRIPNRPAEVIKYFGDIRQSQGEL
                     GHEDPGSTQEKIVI"
     gene            complement(27706..28824)
                     /locus_tag="PKM72_01725"
     CDS             complement(27706..28824)
                     /locus_tag="PKM72_01725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP59525.1"
                     /translation="MKLKIKRIQTYKELDSLKLIWEEFQNAKNGPSVFQSWTWNRAWC
                     EEVLQSQKYANLDVRVAEESNGKIVAILPTYLDKRTIPGISTLQFLAHRMSIYNDILL
                     CEPENQELALAVAESLIESLGPTTIVHLRHLGGESTFTKALLLKNWAQIQCPRLLLRN
                     DPAITDQSKRLGQTRRKAFRSAHNRLMKEYSIEYRVVKSQDFSPAMSELIELHLRRFD
                     AKGQSSLLQGQTLPFLQRAMQDSTNRNQFEIVQLRANDTTVAAVLMVKDRKSYIYFQG
                     GFEPEFSRYSPMRLLLTETIRRGFEELGCEVYDLGDGYGTYKCDWSPTAAMNYFCCRG
                     NNPLYANVFATATRYAFQRRTLSCLSEEVLELSGIQPH"
     gene            complement(28882..29790)
                     /locus_tag="PKM72_01730"
     CDS             complement(28882..29790)
                     /locus_tag="PKM72_01730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP59526.1"
                     /translation="MSFVQLGFVSDTNPNEIIIGQSDQSRCVAAFFSYLYEAENEWDV
                     MKLRGLKKQSTTMHLLEDFLKKNPRRWIWDTSIESPFLPIKTDWENYYKNLSKNLRNS
                     IRRGIKDIQKCGTLKTLDYSTPDRVDVGIEIIYEISERSWQAQEGTDISSGKDGRFYA
                     SFAKAAADRGWLNLKILFLDDHPIAFEYNICFGGTVYELKSGYDQAYRQFSPGKILMR
                     YNVEYFISNKFSEIDFMGASDDYKLQWTKLVCPYTNLWMYAQKKKSMVHYFIRAKIIS
                     KVRHYKSIEKLQRKISRLGSRPDKQL"
     gene            complement(30265..31743)
                     /locus_tag="PKM72_01735"
     CDS             complement(30265..31743)
                     /locus_tag="PKM72_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNP59527.1"
                     /translation="MNEDANNDVGAGARIFRNFGYLTIGKLLADGCTFLFFLVLARTY
                     GQEGIGQYSFAMALTGVLSVFADFGIQTLSIKNLSRYRDQVTTYYGRLLAVRLLLSGM
                     VISLLLGMTALYPFPDEMKLLIIVIGAYQIFLTLVEGFGAVFVAWEDMHITSLFEVSL
                     RITTALTGMVIVYLGGSLLLAVSSLPVMTLLHVALGYHLILRRCGALRISVRTAGVRQ
                     VLREAFPYALYSLLDLFSSRVALLFVGFMLTVSATGVFNVAYRFIVVLLFMPIFAGLA
                     ILPLASRLYVSSLEEFQELYRVTLNVAIVLGLPMAGGIWLIAPRLIELLYGLEFVESI
                     VILRWLAWLVPVVFLRCIMRVFLIASDRQEAVTRSQFLVACVGGILHYWLISRFGLQG
                     AAVATLLAELLLIGLLGFQLKKFFVWQNVGIRIVVSSTAVAVFLMILVFWFSLPLSAT
                     ILCAVLIYSGVLVLFSDIRQDELRLLLNYLSHNRPIQSGLTK"
     gene            complement(31846..32970)
                     /locus_tag="PKM72_01740"
     CDS             complement(31846..32970)
                     /locus_tag="PKM72_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNP59528.1"
                     /translation="MTIRHFKRSFEKNRNELLGLGLGRYPLFVRSDVQPEQIPVFQFH
                     DVSTATLQPILEFIARNDYVTLTGDEYYERTVGASPRGDREVLLTFDDGQASLYTVAF
                     PLLDKFKQRAVAYIVPGRVPDGVTNLSGNDLDSALCSWPQIREMHSSGVIDFQSHSLY
                     HHSIAISPTVVDFKRPGLSTSFLESDLAPIHQQTLDSSRTELHNFWGSPIYQWNARMC
                     ANPAFIENVQIEDLCVRYVADHGGQEFFYDRDWRRQLKQIIKNAQGKCAPIEFETADE
                     QRNAILTDLCRSKAAIEANLPGSRVRHFCFPWYRGSALAAELSLEAGYVTNAWGSLLP
                     QFVDKDEIQPLPIPRLQPKYIYRLPGVGRRPLWRLFGGAM"
     gene            complement(32980..34089)
                     /locus_tag="PKM72_01745"
     CDS             complement(32980..34089)
                     /locus_tag="PKM72_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNP59529.1"
                     /translation="MKIGICARTWGENGGIGVYTRSVIEAILHLDTRNEYHILYSNKA
                     HLGSLPGTDRVREVYLSAKGKWMWDQWAVPRYAHREGLDLIFHTKFAIPFLARCKTAM
                     VLHGTERFVHPEFHQTADLWFFKTVYPQYLKQASLIIAVSNRAKEDIISYLGIDPDKV
                     KLAYLATDPIFRSITDPRQLDPVRVKYKLPSRFILFAGHIYPGKNFTRLLEAFSKVRK
                     HNDVHLVVAGGMRWKYQEDMKTLESLGLQDCVHFAGYVPQHELVAFYNLAAATVFPSH
                     YESFGLPNIEANACGCPLVTSRTGGSPEAAGNAAIYVDPLDVAEIANAIGRVLTDDDL
                     RQTLIAKGYENAARFSWEKTAKATLDAFEWVVAPA"
     gene            complement(34086..34346)
                     /locus_tag="PKM72_01750"
     CDS             complement(34086..34346)
                     /locus_tag="PKM72_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNP59530.1"
                     /translation="MSNTHAKVIEFLSGVLQVEIPPDIDDLHRREFSDWDSINHLRLI
                     MELEEMLGVSLEDEQAADLSSSRQIEALFLKHGLTLPGEESS"
     gene            complement(34358..35926)
                     /locus_tag="PKM72_01755"
     CDS             complement(34358..35926)
                     /locus_tag="PKM72_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNP59531.1"
                     /translation="MKSLPIVWQEAEGLWPDKIALITAEEELTYRELARRIRRVSNAL
                     IRQWDVRPGHVVALLAPNSVEFVVSYFAVTAIAGIVHPVDERLKPEEIGFLLEDSGAR
                     CAIVHQALWSKFSTEWKTLPALEHVLSIGKVETHSNVECFDGWLNLPADPGDLHESGI
                     SPAPDAIAEFMYTSGTIGKPKGAMRSHGNARAASGNARRAFGYRHEDVIAIVMPLSHS
                     SALVSQMLPMIEVGGTAVLVERFDAVDLLARIRKWNVTCFRAVPATFKMLMVYPEFHA
                     GYLPSLRLLMNSSAAIESQTHLAIKERFPNVELINSYGLTEASTCTILSDTVGLEHPD
                     SIGVPIPGVEMAVMDDEGCPVEEGLTGEIWVRGPHACLGYHRLPAETEALFAPGGWLK
                     TGDMGHKDSLGLFYFHGRKVDVINCGGRKYAPVEVEQCILELKDVAEVAVLGVPHRVL
                     GQVAKAYVVFRDRATGDLKAVTRHCARSLPSHKVPFFMEAVDALPRNSLGKMLHRELK
                     AESPPALHKVSRNG"
     gene            complement(35923..36663)
                     /locus_tag="PKM72_01760"
     CDS             complement(35923..36663)
                     /locus_tag="PKM72_01760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNP59532.1"
                     /translation="MNERPLVESLGDAKLSPYRQYLSMFVGEESLGALAKYELYISVL
                     GRLPGAIGYFVRGKCYPGLLGSVGRGTVFGAGGVLRCPGRIHLGRGVMIDDCVVLDAK
                     GHGSSIVLGDQILLGRNSILSCNDSSMSIGNFVSIGPFCFLVSRSHLTIGSNVAIGAG
                     THMLGGGHAHDDPDVPVIQQARTSKGITVEDGAWIGIGAKILDGVTIGKNSIVGAGAV
                     VSKDVPPWTVVLGNPARVVEKRKQVNGA"
     gene            complement(36747..37736)
                     /locus_tag="PKM72_01765"
     CDS             complement(36747..37736)
                     /locus_tag="PKM72_01765"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNP59533.1"
                     /translation="MGRVMVTGGKGFLGTHLVRRLLADGQEVRVFAHRGSKQGEASPA
                     ITECEVIWGDIRNLPDVEQAVKGVEKIFHLVSNFRKGGSDKTDAYAVNVEGTKNVLGA
                     AKKYGVGRVVHCSTIGVHGDVQSIPAHEETPFNPTDLYQETKLLAEQYVWKFHQETGL
                     PISVVRPISLYGPEDLRMLKLFRTIKKRQFIYIGQGNVLFHPAYIDDVIEGFLLCASH
                     EKAVGEAFIIGGDGYLPLHDLVDRISAQLQVPAPSMHIPLRPVEWLAGLCESVCTPLG
                     IEPPLHKRRVSFFKNNRAFSIEKVKRVLGYAPRVSLEEGLKRTIRWYEDHGLL"
     gene            complement(37736..38536)
                     /locus_tag="PKM72_01770"
     CDS             complement(37736..38536)
                     /locus_tag="PKM72_01770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP59534.1"
                     /translation="MTEHAEPIWPLRLFKKSVLKQRKYKELTEALGETTTLQCLDIGA
                     DNGVISYLLRQRGGHWKSADLDQTTVETIQDLIQEPVFQIDDRPTPFADNEFDRVAVI
                     DFLEHIHNDEAFIRELHRIVKPYGLVVLNVPHIKPTLLRKIRLAIGQTDEKHGHLRPG
                     YTRGDINRILGDHFQVVSEKTYSKFFSECIDTLITFAFDLLKRGKRTSAKGLVVTGKD
                     LKQYQKMFTMYSLLYPIIWTISKLDTALFFTSGYMLLVVAQNKKDANA"
     gene            complement(38539..40212)
                     /locus_tag="PKM72_01775"
     CDS             complement(38539..40212)
                     /locus_tag="PKM72_01775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNP59535.1"
                     /translation="MLTRNLPRLANARYDVAVVGGGIYGACVAWDASLRGLSVVLFEQ
                     GDIGSGTSANSLKIIHGGFRYLQHGDVTRMRESMQEQMALMRVAPHLVHPLPVLIPTY
                     GHGLRGKEVFACALAINDLIGFDRNQLDDPQKHIPRGRILSRRQVLELLPNIPEHDLT
                     GGGVFHDGMVYNSERLLLSFLHSAESMGAEVANYVKVVGYLKTKDQIRGVEAEDQLSG
                     ERFQVEANVVVNACGPWLDQTEDMLSDRPGYHKIPWVKAFNIITRPLFSSYAVGLAAR
                     EVYSDDRALLKKGKRLLFMVPWRDCSLIGTSYRSYEGKPDEFRISEEDIHEFLQEINQ
                     SYPHAALTMEDVAMVNGGLLPGAKAESGRRDIQLESDVYIRTREDVGARNVISLVGVK
                     FTTARRVAEHVVDRVFRLLGRTPSPSQTAITPLYGGNIGRFQEFLNQEMKCPPGKLSQ
                     KTVHRLIHNYGSSYRTVLQYLPDSGERNTSDGSESMEILQAEVRYALREEMAQRLSDV
                     VFRRTELGTAGYPGDMPLDLCADILSQELGWSECRTLEELAMVRGRYPG"
     gene            complement(40241..>40564)
                     /locus_tag="PKM72_01780"
     CDS             complement(40241..>40564)
                     /locus_tag="PKM72_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59536.1"
                     /translation="SPRIKGNNTPMKLYSYLASGKAVLATNLMTHTQVINRDVAMLAD
                     PTPELFADGLLQLIRNRPLRVSLGQAAQKLVEEKYSYPAYRMKLNAAYDHLVTTIQQP
                     SESIT"
BASE COUNT        11590 a        10425 c         8661 g         9688 t
ORIGIN      
        1 ctgcccacct ccggcctcca agccggcgcc accgtgtata tcgaccgttc ctataccttt
       61 gcgaccgtcc ccactctcgt ccagggaggt gcttatatca gaaccgccaa tgatgacaaa
      121 gcggcgacca attcgacttt cctcagcttt acggtcaatc aacccgtctc cgtatatgtc
      181 gcccatggcg atcgtattac ctcgaaaccc tcctggttga gcacctttac cgacaccggc
      241 acaaacctgg tgacctcgga tgcgaccttc agcctgtttg tgaagtcctt cccggccggc
      301 acgattaccc tcgggggaaa tgtcagcaat aacaatgcgg gaaatgattt gagcatgtat
      361 tccgtcatcg tcaaacagta gggcgggatt atcccatgaa ccatcaatgg cgaaactata
      421 agagactatc cggagaaaga taagtgatac ctatattatt tcgactagat ataacagatc
      481 caagaaggag gtggtggaaa gctttgagtg ctcagcacca tcccttacag acaactcttc
      541 agatgaggag cagttggtcg aagcctgatc agataaggga aatgcctttg aaagggcttt
      601 tgatctctat tgcttccctt aaaaagattt aaagacacac tcaacaaatt atacgtgctt
      661 taaactggct tatagggttg tattacaggt tagcaaatta agcggtactt aaacttaagc
      721 atccccgtag gagagattta gcctatgatg acgaatccat tgacatatat cttttaccaa
      781 ctacagatac ccagattagg caggggaacc atacaaactc caaccagacg cacagcaagc
      841 agctttttcc gaaaacttcg tccgtcaaca aggttgagtt ttcgaactat gccctaatta
      901 aggttaaaaa aattcctaaa aacttactaa agcttttcta aatattaccg aaacataacc
      961 agtcacggga tgtgacaacc caatttccag gaaactataa gggaatatgt ctaatagctt
     1021 taccatccat taaattccta tgcgtaaggc attctagaga agtcacgaca tagcaccaca
     1081 aggagaaatc catgaaaagt cgatatgcaa ccaatccagc aatactctgt tggtctttac
     1141 tcgcagcagt tttcatgctg aatgccactc agagcattgc cgcgacctat tacatctccc
     1201 ccacagggag taatgtccta ggtaatggca ccagccagaa cccttattat acgctcacgc
     1261 atgccttttc gaagggagga ggccacacgt atatccttaa agatggccta tataattatg
     1321 agggaggcac catcgtgaat ccgccaagtg ggtcaacaac ttcacccacg ataattcaag
     1381 cagaacacga tggcaaagcc gtcattgatg gtgcaggtgt tcgtacgggg gtggacatcc
     1441 gtcaagcggg cacccatcat atcgttatcg aagggctccg cctccagaat tgcggcgaag
     1501 gaccgtccgt ttcggtccaa tccccagatg gcacatcgtt aaccaatcaa atcaataata
     1561 ttaccattcg ccgtacaggt gcgtatggga gtgcggtaaa cacgaacaat aatgtctgga
     1621 gcttgtccag gattcgcgac tctctattcg aagatgtgtg gggctgggga gccggccgat
     1681 atgtcctgag tgtctacggc ggcaccaaag tgaccgtccg gcgggctgtg ctccgatggg
     1741 atcgttggga tggattagcc tacaaacccg gtgacccgaa attcaacatg agtttatata
     1801 atacctatga cagcttggtg gaaaatgtga ttctcctgga tgggactact gcaacaggag
     1861 ggggagatcg tggtggcata ttgcttgccg gaaatagcaa tggtgacacc gctccctata
     1921 ctggctcatc aaataacgtc attcttggca tgattagcct caataatgtc ggaaacggtc
     1981 tggcatctga gtcaggaggg ggaatcaata acaataatcg atttgaaaat gttgtttcct
     2041 ggggcaacga atacgcaatt acggttccta ggaaaagcag taataccaca ctcaaccatg
     2101 tcacattggg tgggggatct tatgggacgt atttcggtgg gggcggcaat caggtgacca
     2161 acgtctccct aaaaaatagc ctcatacaca ataattcaaa tagtggaatc aatggatccg
     2221 ttacatcaga ttacaataac gtctattcga atagtcctaa ttataacggc gcaagtcctg
     2281 gtgctcacga ttccagtata caaccaggat taaaatatct gctgcgggcg gaaacaggga
     2341 caccgaataa aggggcggct tcggatggtg gagatattgg cgccacaatc atcaaacgct
     2401 atcaaaatgg cactttaacc aaccaaaatc tctggccatt ccccaatgag gcacggatta
     2461 aggcggatct ctgtgcaaat gaatcacgag gattctgcgg caaatcatcc ctcacacatt
     2521 atatttggga atttcttggg aacccacttc cagcagaata tgcgaatggg gggggcacct
     2581 cagatacgat tcctccggct tccccaaaag gccttaccct taccattcaa taatatggtc
     2641 taacctggtg cagcaatcag ccatgaaagg tttgctgcac ctcccccttc cgagaaatac
     2701 ttccacccat ccgttagaaa atcacacatc agaacaggcg ccgaggagaa cggaatagta
     2761 ttgggatgcg gcggcatccc aggtcaagtt accgacagac ccgcaaattt ccccttcatc
     2821 ccattccctg gccagtgcca tatccaacgc ctcagataat tggtgactat cgccaggctc
     2881 aacaagaatc ccattagttt tcccaatgag attgggaata tcacccactc tggatgccac
     2941 cactggacgg ccacaggata atgcctcaag aatcacattg ggacacccct cacgaagact
     3001 cggcaaacag aaaacatcac atgcaccgat ccatagaggg atatcctgat gtgccttcgc
     3061 accaacaaat tgaatatgcc cttgaaggtc ctgcttgtgg acgagttctt gatacgcttt
     3121 gagcatgggg ccatccccga taatatacag ggttattggc tttctaattt gaacaagttc
     3181 ttggaaggcc tcaatcagat accgaagccc cttctcttcc tcttctgcta atcttcccac
     3241 aaatagaacg cagggaccct tttgtggaag tccaaggatg gtttccgaat cggtctgatc
     3301 ccttggaaaa aataattgtt tattaacccc gttacgaata accgtgacac gtttatggtc
     3361 tatccccacg cctaacaggt gagccttcaa ttcttctgaa acgactgtga ctcgagaagc
     3421 ctgattcaag gcccgaagaa tcggccccct cttgcgcttc tcaaacaaaa acatattcac
     3481 atcacatccg agacctgtca gtaccaaggg cacccccata attctggcaa gcagggcagc
     3541 agccactcca tcaggataaa gccaatgggc atttatcaca tcaaaccgaa aacagcgcct
     3601 cagggcgtac gcgtatcgaa caaggcccag gaataatagg aagctatggt aatggtcacc
     3661 ccatttagga ataaacggat atttgggact caagacgggt atgctatcga tagcatattc
     3721 aattggaatt aatgaatacc catgccatct ctccaaaaat ttccatcgag ggagcttcgg
     3781 gaaccagggt tgtgggcaaa cgacgcggac ctcgcaatag ttcgttaaat cttgaacaag
     3841 ttggtggcta aagactccct tagttggatt cacgggagtt ggaaaaatct gagaaataaa
     3901 cagaactttt aaggacattg gcatgacgaa cagaacaccc atcaaaaatg agagtgatca
     3961 atcaatgtac tttttgtgcc acatttccaa agcgacaagg gaaaaaagtc ttcggctcaa
     4021 atcttccttt ccctgccgat gtcgcttcaa agcattatca atatacgact cttgaaagta
     4081 atttcgtgtg agacttttgc tatccagcaa gacatcacga acctgatgat aaagtttttc
     4141 cttaaaccac cttgccactg gcacgggaaa tcctttcttc tttcgataaa taatctcatg
     4201 aggcaaaact ttttccataa ctttttttaa ggccaatttc ccctcccacc ctttcaatcg
     4261 atactcatca ggcaaggaca tggcaaattc aagtaaatgg tgatccaaaa atggtacgcg
     4321 cagttcaagg gaattggcca tggtcatctt atccgctttg accagcaaat catcaggcaa
     4381 ccaagactta atatccagat agcccatttt cctcaatccg gaactttggg aaagtgtgcc
     4441 aagaagtttt tcaaaaaata catcagaatc attgcctaaa cgagaagcaa agcccggttg
     4501 atacattttc cgcttgatgc tcggcgtcac atcgttagag attcctcgat agcggttaag
     4561 aagaggcatt ctcatccaat caacatactt tgtgtatttc tcattggcct tgaggctcgt
     4621 cgacagacat tcatgaatcc aggatgggag gtagccaaat acacggtgga ttcgatcaag
     4681 ccttgccata attgggtaca ggggatagcc ggctagaatc tcgtcacttc cctcccctga
     4741 gagaataacc gtcacatgct ctttggctaa ccgagagagg tgaaaaagtg caattgccgc
     4801 actttccaca atgggttctt ccgtgtactc aagtaacaaa tccaatgatg caaagaattc
     4861 ttccgattca aggatatact cgtgatgttc cgtgtgaaaa atatttgcaa tacactgggc
     4921 atagggaagt tcgctggcat ctttgtcatc ctgatatcca acagaaaagg ttttgaccgg
     4981 ctcggtcata aatttactca ttcctgcaac caccgcactg gaatcaatcc caccacttaa
     5041 aaatgccccc aatggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgaaa
     5161 ttcaagcacc ttgagaatgg cgcgcacctc ggaagcaaag aggaactgat gctgatccac
     5221 ataaaagtag agtggcttta tcccaacccg gtcccgagcg agaaaaactg atttcctttt
     5281 gacatcataa agggcaaagg caaacatgcc gtcaaaccat ttgggacagt caattccata
     5341 ttcttcatag gcatgcacaa taacctcagt atcacacttg gtttgaaagg tgtgcccctt
     5401 ggctattaag tgctcccgca attccaaaaa attatatatc tcgccattgt agacaaccgc
     5461 aatagtccca tcctcattaa acataggctg tgcgccagca tgtaaatcga taatcttcag
     5521 ccttcggtgt ccaaagccca catgcttatt caaaaatatt cccgcggagt caggaccccg
     5581 atgagccatc gaatccctca tctgcacaag gagattttgt tcgacaggtt caccctccct
     5641 aaacctcacc acaccacata tggcacacat agcatttgac tccgaagaaa aaggttatga
     5701 cattccccca gcaaaaaaca gtgcagtaaa taatcgaaga aacagtacaa atccaacttc
     5761 tatcacggca atccaaaaga tatcatttag agtcaaccga ggaagaaaac catttaatga
     5821 gcaaaaagct gcagcaatac caagctgtac ataaaaaaga ataaaatctg tagtcacaaa
     5881 catcgaggtg gctgcatacg aacaaatgct cgctaaaaga gccaaagcga gtgagcgatc
     5941 cttaggatca gagacttgct tcgatacatg ccacagccct tttatcgaca gatacgacaa
     6001 tgagatgtag ataaacagcc cgggaaaacc agtttcgccg agattttcca cataagaatt
     6061 atgtgcaatg aggctgagcc ctgttttatc tctcgcagcg ttcttaaaac gcatctttcc
     6121 taccccaaaa acagggtttt ctttagccat ctccaaccct gcagcccaaa gatccacacg
     6181 atgtgccgct gacttactcc catcactatc atcaaacccg cgtgtcgtca gaaagccaac
     6241 caacggcgcg gccagaacca gaacaatggc accaaggatc aacgcggtac gtggcttaaa
     6301 tcgtaagacc agcgaagcga acaaggctac gattgtggcc aataatcctc cgcgtgactg
     6361 agtcaaaaga agtccttgaa aaataagtag gctaaaaaga aaggatgtaa tccgaataaa
     6421 gatattccag ggggagaaca taaattgaat gagaaacgga aaggaaatga caaacagcaa
     6481 agccagaaca ttcatcccat cccaaagccc cacccacttt gcgcgaccac cgtgaccatc
     6541 atcccaacca agatgttgcc cggcccaccc aaccccagtc gccacttgat caatacactg
     6601 gtaggcgaga agccctgaca ggaaaatttg gaaccatatc agccgacgga tttgtccagt
     6661 tgagtttaaa aacagaaaca agcacatgaa aataagaaag gatttcgaat actccgtgaa
     6721 agccgcacgc gcataccatg ctccttcccc tccttgcgca aggtttgaca ggctgatcac
     6781 cacaagccag actaccaata catacaataa tggcaacttg cacaaacgct gccatccatc
     6841 catttgaaaa atggagccac cgagcaaaaa ggcaaaaatt atataatcaa caggccaatt
     6901 caacataaac ggcacccagt cttggggccg caaataaagc gtgatgaagt aaagaaatag
     6961 aagaaaagac agcggtgaag gtataggtcc attcccagat gcccggttgg gcccaataga
     7021 gtccacaggc tctagatctg cggtttcttc tttgaccata atgttttaaa tggaatccgt
     7081 ttcgccgttt cggaatagaa tagcctggag agttgtattt aattcatttg ttttttgttg
     7141 tagcttggaa agatctccca aattttgcta aatagaaaga aggatcgagc gcgactccac
     7201 tttccgcagc tttgactgcc tgaaataatt cgtaggcagt cacataatgg agacagtatc
     7261 tttgaccatc gttatattcg tcctccagat aggtccacat acggtccatg ggctctccaa
     7321 gcaaaaccgc catgttctgc tcatcggctc catgagtatg aagtttcaca aagatccaat
     7381 tgggctgtcc ttgtacgtga atatgagtct taatccaaag gtcaacgcgt tctttggagg
     7441 gagggttatc cccggagagt tctccatttt caatcctggg gagtaaccct aattttcgag
     7501 atttccaatt caaccccaat ggaccttgga tcaacatcaa atctccactg gacttcctcc
     7561 cagcttcaac agcaacaccg gtgttatggg atttcggtct cagggggtca tcggtggcat
     7621 aatacacaga gttaattgtg gcaacctgag actcgtcggg agcggaagga aaggtaaaat
     7681 ctgcgtaaca ccccgtctca cgcaaaatgg agatctcatt attgactcca caccatctcc
     7741 ctgcaccacc actattatct aaggcccagt ttccatggat aaaaccatat tcgatctctc
     7801 ctgattcagg atttttacgg aggagatcgt ggcgctcatg aagaatggtc ttaaattcat
     7861 gaagtgtatc cctcaaatgt tcgggggtat cattatcatg atgtagatga atctcaacat
     7921 ctccaaaccc cattccacat aaagtggcga gatgatctaa atgagcaggt cgatattctt
     7981 ccgcaggaaa gaagaagcta tgctgtggac atctcccact cacatcccga tgcctggacg
     8041 cgatatgcgg ataatttttc atccattttt ctacccgggc acattcttgt tcatacggca
     8101 ccccaccaac agcaggttca taatggtcgg tcacacaaaa aaatatatga atcggccccg
     8161 cagtcacctt tggacgagaa cgaaattgct ggatgacata gctcgcaatc cagagatgca
     8221 tctgcttttg ccgcaataca agcccgacaa atcccaggat gatcaaaaac ccaccgagca
     8281 gtccagatac gagatacact tagccattct ccagggttag cgaatcctaa cgttcggctt
     8341 tacgccaaag actggtttgt ccggcactaa agaaatgaag gaaaaatccg tacaacgcgg
     8401 cggcattccc catacagaag tacagcggca cataaaaaaa ggtagcgcgg ccgagtgttt
     8461 tcatgagaac ccatcccagg accgcagcaa gataaaaaga cacttgcccc cagaaaaggc
     8521 tgtgatacca aagggaggga atcagcgcac tgctcaacag catggttaaa agaaaaaatg
     8581 ggatcaacca gcggaataat ttgtgagaga taaactggaa ggcaaagaac gtctttggcg
     8641 gaaaaaacat cttggggaat tgggtgagaa tctgataccc ggcagatgca tagcgaacct
     8701 tgagaaaaaa ttcatcatga agcgtttttg aggaatattc ggctgaagtc ccctctcctt
     8761 cataaatcac tcgaaatccg gcttcaataa ttttcagaga aagaaacata tcgtcatgaa
     8821 caatgaagga tggcataggg gcaaaaagag ctctgcggat ggcgaaaatt tcaccatccg
     8881 cacccacgat ggaacccaaa cggctttccc ccaatttaag ccaactttca taattccaga
     8941 aggccgtctc cccttgactg gtttgcctat cgcagtcttt taaaatgacc tttcgacccg
     9001 aaaccccacc cacagaagga tcatggaaat ttcgtgtaat cttctgaatc accgttggct
     9061 catagacggt gttagcatca gaaaataaaa caatctcccc agtagccacc gaaacggcat
     9121 gattcaaagc tgccgtcttt ccttgcctgg ccggtcgatg caagacggtg actccctgtt
     9181 tttcatatga ctgagcaata ctcaccgttt gatcagtcga cccatccgca acaacaataa
     9241 tctccaattg cccttctgga tatgccaacg ccatagtgtt ggccattttg ctaccaatca
     9301 ctttctcttc gttataggcg gcaataatca tagatacaga aggatagaat gcgccttgct
     9361 tgatgggcct cggtcttatc cgtgcgaaaa cccaaacaag aagtggataa ccaaaatagg
     9421 tatacagcac cgccccaaat gccgcccaaa agagcagttt catcccaaat ccttttcagt
     9481 acagtagaga ttcatgcaac acctctcatt tccttcaagg agagcctacg gccgttcttc
     9541 cctctcaaaa aggaaaggag agattaaact ttattaaagt taagtgtatg ctgtttatgt
     9601 cggtaatttt tcgacaatcc taagcggatc catatagcga ggaaggaaaa accagggtct
     9661 tcaactctgg tcaataagtg cagaggcaaa ccgttcaacc ttttttccgt tctcatgcca
     9721 ggtatgctga cccagaactt ttttcctggc acgggcacct aactccatcc gcagtgcact
     9781 atcgcccaat aacagtgaga gggcctgccg gagcgcacca ggatcgccac attggacgat
     9841 caatccatcc ttacgatctt caataatatc tcgaatgggc agaaggtccg gcgcaatcac
     9901 cgccttcccc atgcccataa actcaaacaa gacgatcgga gaaccaaaga catttgaatc
     9961 tggaagcaca cagatatcca tagccgcaat atacgataac acctgagatc tgggcacggg
    10021 gccagtgaga aacacaacct cttctaactt gctggctcga atctgttcga tgagcccatc
    10081 aacaaccggg ccattgccta ccagcataag ccgaatatgt ggatattcct tgtgaacatc
    10141 tccgaccgct ttaataagca gatctaaccg atcccaggta tcaaaccatc ctgcaaaacc
    10201 aacgacagta tgaccattga ggtgataccg ctttcgaatc tgatctcccc attccgtcgt
    10261 ttctatcact tgggcatcca ccgcgttggg aatcacatgg actgcacctg gttttccgcc
    10321 tcgctttaac acctcttctt tcaaaaagct cgagacagtg agtatcacat cggccttttt
    10381 gaatatcatt ttttcaaacc attgagagac aggaaccagc acctggtttc gagccctctg
    10441 aactccgact acctcattca cctccagcaa aacaggccac ccaaaaaatt tggctaaaaa
    10501 cactcccata aacatgaaaa atgcataccg ttcatagtac acatcttggc ctcgccgtag
    10561 taaaattggc cccaatcgaa tcatcaccac cacgttatag ccgatttcaa gaatttcaaa
    10621 gaaaatatca ggaacccaac aactcacaaa tttccagatt cgggttatcc ctcgtacctg
    10681 ggcctgacct ttatcaacgg gcgaggcgcc ggccatccgg agtggatcta caccagacgg
    10741 agatgccacc tgaatcgtat ggcctagcgt ttcaagagcc cgaaccatac tggcaatatg
    10801 aaccccttcc cctcctcgac ccagcgttct gtgatgataa agaaatttca ccgtggctct
    10861 ccgtcaaacc gtgagcattt ttgaaatatg aaccgggttc aggttttggc ggacgataac
    10921 atccaggata ttcggaagtg ccttgaccgc agagggattg gttccgtgat ataacagaat
    10981 atccccggat tgaagagact ggcattgtag cgcggcaaga atttctcctg gactctctgc
    11041 ctggaaatcc ttgagatcaa cattccacat gacacacgtg agacgtgcaa gccaagctcc
    11101 cccaatagag cccaaacata attttccata gggagggcgg aataatcgac aggggcgtcc
    11161 tgtgatgctt tgaaaaagtg cctgagtgcg ctcaatttcc acgacgactt ccttccatga
    11221 caagtgatcg aaggaaggat gagtaaacga atgattcccc aattcgtgac cttcctcaat
    11281 cattcgtaaa accaactctt tgtgccgctc gatcttttca ccaacaagga aaaatgttgc
    11341 ggtgacacca taggttttga ggattgcaag aatttgcggg gtataggttg gatcggggcc
    11401 atcgtcgact gtgatggcaa tgtgacgagt atcccgctcc ccattccata acatccatcg
    11461 ccgtggaaac ttctgagcta cctttttcac gatattcttc aagaatgaag gtggtggctc
    11521 catctcatag agatattgat aggcctcggt catcggagtc attccaaaat ggtctcgaat
    11581 acggtcttgg gcattgttgg ccattcgctt ggcttcgcca ggatcctgaa taagtcggga
    11641 cagagcttta gcaatatctt cgggattccc cggaggaatt aaaagacctg attcctgatg
    11701 aactataatc tcgggaatac ccccaacatt cgtcgccaca acgggaagac cgttggccat
    11761 cgcctccagc aatgaaagtg gtatgccctc ggtcaaagat ggaagaacaa aaaaatcaaa
    11821 agtggacagg aagatttcaa cattttcctg gtaccccata aattctacgc ttgaagagat
    11881 accaagaccc tctgctagcg cttccaattc accgcgctga ggtccgtctc caacaaggat
    11941 cagtcgaagg gcaatgcctc gtgcctgaag gaccgaataa gcgtgcaaaa gatgatgtat
    12001 tcctttttca ggagagagcc gggctacggt ccccaccacc cattctcttt gattttgaaa
    12061 atcgggggcc tcgcgaaaag ccatgttctt cccattgtgg gggttcgaaa atttcttttc
    12121 ttcgatccca tttaaaatcg taagaccttg aggagaagag attccatact ccccaaacgc
    12181 ccgcgttgta tcttgagaga caccgacgat tttatgaaaa aacagagaac cgactcgaaa
    12241 cttcagacgg tccttccagt tgtatgttct caaccagata tcatgttgcg tatgaatacg
    12301 aacggcgaca gacatccacc acgctgccca cccggcatac aacatcggat caagactgtg
    12361 ggtgtgcacc acggtaactc cttcccgctt caatactttg accaaacgac caatcaaacc
    12421 caaatcaagt cctggcctac gtttgacgac cacaatccga cagccggcat tttctaccat
    12481 aggagcaaga acccccggtt catctaaaca aaggacaatc gttggcaaag ttgaaagaat
    12541 aggagaacaa acaagcgtac ataccaaccg ctcgagtcca ccgggttgaa gacttaaaac
    12601 cacatgagca acttttgttt gattggccaa tgcattcctc tgattaaagc ttgttgaaaa
    12661 agcctccatt caggacaaag ggattcccct atatagttgt tccccgcaac cgccggattt
    12721 cttcgacagc gaaatgccac cctgcctcga cttctgacca gttcagacag cctgcactta
    12781 atatgagaaa aggataacaa ctcacaatag ctatcccggc acccaggaat cccccatatc
    12841 ccataaacag aattccccaa ttgagtccta aaaggagagc acaaatccct ccaatttttc
    12901 ccagagcccg ataatcatat tgaatgggaa aaagctgttg cgaataaaac agcattaaac
    12961 tcacactcac gacttccgcc aaaagatacg caaaccctgc ccccattgcc cccaaacgag
    13021 ggatcatggc aacacttaag atgattttca caaagccggc caacacagtc acataaaata
    13081 agtacttcgt tttttttaaa tacaaaatac cggtctgaaa tgggtagccc aaacttgaca
    13141 ccaaaagacc cgcggccaga atcgggataa ggtgttcagc cggccaatat tctaatgtcg
    13201 tcagcatccg cagaatagcc ggtgaaaaag ctgaaattcc cagtgccaac aggccaacca
    13261 ctaaagccat ataaataaca attttggctt gcaattcttt cacattttca ttcttcatta
    13321 ttgaaaatcg aaaagatcca taactgtttt taaacggctc gagaaccagc acattgccca
    13381 attccacgaa cttcagcgcc aaagcataaa gtccgaccgc ttcaagtcca tacaaagcct
    13441 tcaggagaaa ccgatctgaa tacagaacgg caaccccaac aatggtgctc aagagaaagg
    13501 ggaaactata ggtgagcacc tggcgaaact tggcttggtg aaaccgtagc ccgcattcat
    13561 acaccgtggt gccaacaaca tagacccacg atatcccaac gctcatgaga tttcccagaa
    13621 gaatccccca cactccccat ccaaggatag ccaccgagta ataattacat cctacctgta
    13681 aaagtaatcg cagaaaggaa gccaccacaa atcgggtgga atactccctg gcccggaagt
    13741 acgcgagacc aatttccgtg gataattgta gaatgagaat aaggaggcca agctgtagga
    13801 gactcgcgtc ttgtcttgag ccaagcagaa atcctgacaa aatcggtgtc gtaaaccaca
    13861 acaaggcgac cccgctggag gcaatcacac tggatccgac caatgtcgta ctcaccagaa
    13921 attttcgttc caactcttct tggaactcaa aataaaatcg caatgtcgca tgcgccaatc
    13981 ccactcccag caaagaagag acgagtgccg tgaccccgta aactaactcc agcgtcccat
    14041 actcagaagg cgtcaaatag cttgtataga gcggaagaag aagaaatgct ccagcgcgat
    14101 tgacaattcc tccgactgca taaatggaag aatgcttgaa gaggcgcttc atatctgact
    14161 gcatattcat aagtcccatt caaaagcaaa accttcaaca gaaaccatct ggctgtttat
    14221 gaccctatat tcaatcggaa cattatgaaa cggaacagtt tgcttcagtg accgtagcta
    14281 acgccttctc aaaagcagaa cgcaaaattg gcatcatggc gtgagagaac caatcttctt
    14341 ccgatttatg ccaatgatcg aaatgctcat gaaattgata gagaagcaga atgaccgcat
    14401 aaattttctg ccgatctcca gggcctaaat cgagtgcgtg ccaatacgtt tggacacact
    14461 cattcttaaa aagatcttct tcaaaggcca ctctcacaat ggcttggtaa aagggaattc
    14521 gctgcttcaa atagacttcg taactcaagt accacaataa atccatcaac ggaaaaccca
    14581 tctctcgcga caaatcccaa tcgatcaaac cgacaacttc cccggactca ttgacaagta
    14641 aatttgtccg cttaaaatca ccatgggtcc gcaccagaac cgcttccttt cccagcaacc
    14701 cttctttcaa tagggcttca acctttgcaa ccatctcttt atcggttcca ttggaatacc
    14761 ccattaaatg gcaaagagaa gcacccacaa ggcgctcaaa aagcgcgtca tcaagaatca
    14821 ctttctgtgc tgttgcggta tgaagttgca aaatgaagtg aagggcctga gccgtcatcc
    14881 tgtcagattc ccacgaacat tttcgggtcc ctgttggttc aagaccgggt agtttggatt
    14941 cgacaaaata cctcaaatcc ccaaaacgac gaagattgta gggagctgga atggccacag
    15001 gagctttacc ctgtaacgca tgtaaggtct cataattatt tcgacatcga aacaaaggct
    15061 cgtcgcctaa aggaaaccgg acaatccgac ctggaagttt ccccacaagt gtattggcag
    15121 ttccgacata cacttgaata tctgatcggt ccgattggtc aatctcaggc tgactcacga
    15181 tgtcccctat aacccctgaa gaccgctccg ggcaggactt tcctacaatc aaaccgtacg
    15241 actcccctct tagcactttt ttcaatgtgg accgaccttt cgagacccac gactttttgt
    15301 ttcgctcctc aaataccgct tcaacaggcc cgcaagtcgg ataaaaactg agtctgttga
    15361 gaactctgct tgaattcctc accattgcag atccaaggcc gcgttggtat acccgggttg
    15421 cctcatagcc aatgtcagag aataatggcc cttccctcat tcccaaatag atcattccac
    15481 caggagtcaa taaattgaag agttcggaaa aaaaatccct agccttgcgc acagtgccta
    15541 cgccggcttc caaccaaacc tgcgggtcag gaaggcacac aagatcaaag aggccttcct
    15601 gaaaagaaag ggactgatta agtccataga ggaccgtctt aatttttcgg ccctcttgcc
    15661 tggcgcgagc aatggcacat ctcctgctag gctcatcccg atagaccccc gtcacattga
    15721 taaataaata ggatagggcc accgttgtgg ttcctaaccc cgcatcaaga accaggacgg
    15781 tatcctggct tgttcttccc acccataaca tccatgctgc ctgggatccg aggtggattt
    15841 cccaaaagag tttctttcgc tcttcggagc ccaattcatt cagaatcttt ccgacaccgt
    15901 ccagccagcc atatccttcc accaaatcgg caagacgttc atgtatcgtt gaggccatca
    15961 cccgatatcc ttgcgaaaac caatattccc ttggaaagga cgaacaaggc ttttcaccat
    16021 gtgtttaatc aatccccagc cgaaccggac ctggtggtag aggtttgccg gatgcaattt
    16081 catagccttg gcttgcaact tccaggcatg cttttcccca acaacaaagc tactctcggc
    16141 ccattcacta taaatcttgg ccttacgtat gttgtaattc tcgagagaat actcatttct
    16201 gtacatttgc aatatttgaa attgcgcaga atattctttt cgcaagcttc catgactcct
    16261 ggattttctg agaacccgat attgaccaaa atattcatag ggcaggtaaa attttgtttg
    16321 gttcgaaatt cgtatccaaa aatcatagtc ctccgccttg agatgtttat gctcaggatc
    16381 aaatccctgg attttttcca aaacattttt tctaatgatc gcagtcgaca tgtctatacc
    16441 gttcacatta ccttccatca gtagaatctg cctaagattt ataggcttgt tatataggtc
    16501 taaataacac aggcatggtt cgatttttct tcccaattca tcaatatttt ccaacaaccc
    16561 cccgacaacc cctacatcag gatgcctatg cataaggtcc atttgacatt cgattttctc
    16621 aggaaaccaa atatcatcgg cgtcacaaaa cgcgacaaaa tttcccctac tgtgggcaat
    16681 tcccacattt cgagcaatgg ccaccccata gttctcctga tctatccaat gaatccgacc
    16741 agaaaattct tttaaaatat ctctcgagga atccgtagag ccatcattta cggcaataac
    16801 ctcaatttgt ttataggtct gacataggat ggaattaaga gtttcagtta aaaatttagc
    16861 ggcattatag actggaacaa ccacagagac taaaggtaaa cgtacaatgc tttgttcagt
    16921 aaaattcaat ttatggccca tgcttatgat ggatatcgga caactctgac aacctgtatt
    16981 cggtgatctt ttccggccac cccactttgg atcaccgaat ttttttcacc aaaaacctcg
    17041 gtcacccatc caatcagcag tgaccggtat cttggtttag tcaaaatcca gaccgttcca
    17101 ttaatttcct gaacagcttt ttgtacggtc tccttattat gaagaattaa atttccccat
    17161 cggtctttcc ttcctcggtc ctgggaattt tcccaaaata aggaagactg atcgtcataa
    17221 aatatatagt tctcaggcgt gagataaaat gacgaagaag ggacggcatt gagaatcagg
    17281 tcgcccggct tccaaccttc attaataaag tcggcaggac ctcttacatc agcccgctga
    17341 taatagtgaa cggaaacttt ttcgggaaca ttcatccgaa agttcacctc ggctttcccg
    17401 acattcatga tatgtgcgaa ggagaaatct tccgagcaga gcaatagtcc aaatggcagg
    17461 aataccacgg cacacttcgg aattcgtccc aaattagtat tggggaaaaa tttcgagtct
    17521 agccatgatt ttaagaccag ccccccttca aaccaaagaa gaagaaccaa gggatacagg
    17581 aaaaatgaat atcttgtcga gttgtacatc gtattgatca ctcccaagat gaggacacaa
    17641 ctaactaaca ccagaaggag aaagtgattg ttcaggaact ggcgggattc tgtcgaaccc
    17701 cacaacacca taaatccgaa aagacaggac accatcagga aatagggcat cacatcaagc
    17761 cagagaaaga caaactcctc catcactctt ggataatgaa aaacaatcgc aaaaatttta
    17821 tgcaaagata gactttctgg tccttgtgta accatatacc cagtagtcat gagaaacccc
    17881 acggtaagcc agaatccaag gcatgcgacc aggaatatgg caatactcct ttcaattgac
    17941 cttcgtaaga ccgactgtgg atcaatggtt tgagtgaaat atgatacaag tagaatggcc
    18001 agcacgactc caaataactg gaataggccc gcgccaagta acgctacggc tatggttttt
    18061 tgccgccaat cccataaggc atttttcatc cccatatagg cgagacctat tcccatcagg
    18121 acaagtacca tatatcccac accccagaga tctcctcgca ggaagacaga ctcccacaag
    18181 gaaatgtctg gctgtagaag agggcctccc ccctcctgag ggagaacgaa tccctttggg
    18241 tactcgtttt ccgaacctcc agggaaaagc cacaaccaca ccttcggaaa aacaacattc
    18301 aaaagaagaa ttgcactagc ccaaatacca taccgtaact gatttacaag acttacccct
    18361 cgaatcaata gtggcaagaa cagtaagcac gccatgaaaa tcgaaccctc atagatgacg
    18421 gtgctgatgg aagcaatggc atatgaccaa agaattgccc gttttcggcc aagaaaaaat
    18481 ccttcatgaa aataataaag aaaccaaaga aaggcacatt gaaacataga atacattcgt
    18541 gcaaatcgag caaattccac ttcccaaatt gatacacaaa aaaaagcaac tagaagaagt
    18601 gctcctgcgt ttccaattcg ttttctaccc aacaaataga gaggggggat cgttaacaga
    18661 ttaaacagga ccgctggcaa tcgaagcgca tattcattca gaccgaatag gcctgcactg
    18721 gcagcttcga agtattggaa aagcaagcct cgccaataca tgccaccatg gggatataaa
    18781 gggatcccct tttgctgaat cagttcaacc gattttgcga tataaaattc atcgacagcc
    18841 aggcaccagg tacctaacgc atcgaacctg gccaagaccc caataaaaaa ggcggcaaga
    18901 caaacaatgg cacaacctaa ctcgcttttc gaaatggata cacctggaga ggaagccaga
    18961 ggggaattca taagaacaga gtgaccagaa atcgtgttcg agctacctaa cttcagacta
    19021 tcgttttctt acttgacatt gatttggcga ctattcttaa ggttttgcct ttaagagatc
    19081 tttgatggtg tagtctttca tcttcctggc atgagtgaaa acgatcattt caccaaggag
    19141 gccgaccgag gcggtttgga ctccgagtac catcaatagg acacccagga ttaacagtgg
    19201 ccgatcggtg agtccagtga gaccaaagag tttttggata gccaaaatca tagaaatgac
    19261 caggccacct gaaaacaatc ccgctccgat cagcccaaaa aatcgcaatg gtttttttgt
    19321 gaatttaaag aggaaggcga ccgtaatgat gtccagaaaa cggcgaaggt atacgccggg
    19381 ccgaagcatt cgggagtggc aatcggccgg atgctgggcg acatctcgct ccaccacccg
    19441 gaacccccgt tgataggcaa gcaagggaag gaatcggtga agatctccat aaagattgac
    19501 ttcacgaacc acaggccttc gaatgccacg aaggctgcag ctcatatcat gaaactccac
    19561 gccagtcagc aatcccatgg caaaatggaa gatgcgattt tgcagacgat tgatccaggg
    19621 atcttttcgg ggataacggc gagtcgccac caaatcatat ccctccttaa gggaatccat
    19681 caccgtgtgc agaccttcag gcagtgtctg aaaataggct gataacgtga taatgacctc
    19741 agccttggcc tcctgaaacc ccaccatcag aacagtggcc tctccaaaat gtcgctgaag
    19801 cggaaccatt cgaatcggtt cacccttggc tatcagggca ttgagagatt cggcttgttg
    19861 ctcaaaccct tcgtccagga cgaatataaa ttcaaaggag ggaaccatac gacgtaagat
    19921 gtccgcatgc agacgataaa cctcatcgag ggcatcacac cgttctgtta ccgggacgac
    19981 aaccgaaacg gcaacctgca atggctctga agtgacacat gctggatgga cggaatggac
    20041 agtcatgaga attcgccttt taatgttaag catggcaaag caaaagaaat gacaaatggg
    20101 agaaggtagg agagaaccct gtgtatttgg gatatgcatt ggccttcaaa aagactggca
    20161 atccaatgaa agtccacccc cgtccggaca tatgcgtgag gcggtcgata catcattcca
    20221 aaccgattta ggtcattccc ttgccaattt tgaagactgg acgaaagaat cattgagtct
    20281 gatctctcca aatcatctga tccggacgaa aatccccgga aaagagaatc atttcaccaa
    20341 gcattcccat agtcagcaaa tgccctgtca gtgcgcataa caaaaaagtc gtagcaggga
    20401 atacaattcc aatggtctct gagaaaacca tgccgaccaa actcagcccc gccaacaacg
    20461 ctccaggaat ggcgagtatg ccaaaccaca atgccggcct ggcggcaaac ccggtcacca
    20521 ttttcactaa aaacaaatcc aaaaacacac gccacgctct tgaaattcca tatttgcttt
    20581 ttccgaacat ccgtggatgg tgggtgacaa ccaattcagt aattctcgca ccggacaaag
    20641 ttgccattgc cggaataaac cgatgcaatt ctgaatatag agctacgcgt ttaataatgg
    20701 aggctcgata ggctttcagt gaacatccat tatcatgaat ggctactcct gtgattttgc
    20761 cgataagcca gtttgcaatg agtgaaggaa atcgccgaga aagaaattta tctttccgtt
    20821 ccttcctcca tccacagacc acgtcatagc cttctgcgat cttggccaac aattgcggga
    20881 tatcactggg atcattttga agatccccat ccatactgat aataatgtgc ccgcgagccg
    20941 ctctgaaccc tgcagccatc gctgcggtct gtccaaaatt ctttcgtaaa cgaatcacgg
    21001 tgacttcagg attttttcga tgaatgtctt caagtaactc ataggtacgg tccttgcttc
    21061 catcatccgc aaacacgatt tcatacgatt gttcattcct ctcacaggcc gttcgtatcg
    21121 cttcataaag gggatggaca cattcctcct cattgtaaat tggaacaaca atagaaagga
    21181 gaggtcgcat catccaaaac ctttatccaa gaaactccga acagaaacct atgcgccttt
    21241 gaataatgaa ctcaaatgac tcacctgtgt ttccgctcgc attccctaca ccgatcaagc
    21301 gtggcacaag gactatttcg ggagaaagtc atgagctccc cttacgctca accacatttc
    21361 tcaaagacaa ggaatcccaa gcaaagaaaa tagggaataa acaacacaga attcctattt
    21421 atcatgagaa ctgacaaaaa tgaatatgta gttatgtttc ggatagggaa agacgaaact
    21481 aaaggtcctt agccaagtga ccgcacaggc caaagtcgtc gagaggaacc tgtcaccttc
    21541 tgaacaactc tattcgttag taggcaggcg aaagacctgc tgtctatctt ttactacaac
    21601 tccaccatgg agatacaagc cttcctacaa atccaagttg aacagaaaac cataggacgc
    21661 tccgtccatt ccatgccact aacatgcggg ggatgggtcc ctggcaacta caacgaattg
    21721 attctgtgaa ctgggtggat aggattaaaa tcgaggtcta ggcggagcac aaaacggtga
    21781 ctgggtgcct cgtcatagaa ggaatctcaa aaagaaaaaa gtcttgtagg tgaaagacga
    21841 tatctgcgaa ccaatctgaa atctaaaagt gcggaaaggg agggcgaatg ggcatttgaa
    21901 aatgatagag agaaaggctt cagcttaatg aaagttcacg actgatcctg gaaatattca
    21961 ctcccattat ctagactgct agaagttgat tctgaaccgt tcacaatcaa tgcgcaattg
    22021 gtaaccttac tcacagatca gccgcatcta gctgctcctg tatgcgcacc gttcattatt
    22081 gatcggcgaa agaaagccac gaggctatca aaaccattca ctcaatcaag agcaagagtt
    22141 gcactgaaaa atgcaattaa ggaatgggcg cgacattcag atttttggga ggttcgggtg
    22201 gcacattgtc tgaattggca atcgggtgcg tgcgaagcct gatgacatta ttattccaac
    22261 tcgggtcatc actgtaatac catttccatt cattgccatc ccgcaatatt ttattttgta
    22321 gtactcctga tgtcgtgatg acccgctcat tttgtatgtt cctcatgtcg tcgaggtgag
    22381 tggtagcaaa atacagattg tcattgctac tcccgggatg actaccaccc tggggattat
    22441 atattaatgt gagagtgttg ttatcatcgt gtaaatagac atcaagtccg gaccagcccc
    22501 agtccaatgc actgataccg cccaattcat tgaggctcca tgcatttgat cctctagagg
    22561 cagattttag ctggccaccc tcagcagtag tcacgtacag accaaattgg ctgccatcat
    22621 aaacccaacc tgcagcatag acttcgctcc cacttgagga atatgtgcca ccgactgacc
    22681 cttccaccgt ccaagctttc ccgtcaaccg acgtcgctcc atacacccga gaatcaacac
    22741 taatattaca tccacaggtt cctccaccac caaagttaca tgtctgagaa ccccctggcg
    22801 attcaacacc aaagtatccg taaaacttcc ccttcgaggg aagatactga atatacgcag
    22861 ttcggatccc ctcttcctgg gaacttacag atacaaaatc atggggtttc aacaatggat
    22921 tacctgtgaa tttcgtaaaa ttcactccgt ccgtactggt ggcaagaccg agagattgat
    22981 ggttgacatc accatccgca tcccaacacc cgttaaaccc tgcgagataa tggagataat
    23041 agatcccgcc cactttcgag ataccactca cggcgatacc aggtttattt tcccaagcaa
    23101 cgcctgaact gttttggaga acaacccctt tttcagtcca atcagagagt ttttccggaa
    23161 tcgtatcaag ggcaaacgcc tgccctagca ttgccaaact tataattcca aggaataaaa
    23221 ttcgcattgt gatacatacg tcatgccttc cttgaatttt caaaattgac acttttcctg
    23281 ttttgctctt ttccattttc atttcgcccc cgtttagttt tttcaacctg tggtaagatt
    23341 tgtggttcac taaaaactga ttgtaagcgc aagcaaaatc tacgccatcg taccaggagc
    23401 atgctggatg cgattcaaag gaatgggttt taccccaaga aatccctttc ttccattcgg
    23461 agaaaaagcc aaatcttaag gcatatcaag gtaccaggaa gtaagttgga gtcgctggta
    23521 atgcaaaaaa gttcatttcc ataaaaatat ggaataagaa aactaaatca ataatagacc
    23581 agtgcatcaa aataaaaacc ttaaacgcag tcataatcat tcatcaataa tacgtagctg
    23641 caaagaaacc agtattgagg ccaatctgtt taatcataaa aattctagag cgttggcgtc
    23701 aggattagat tgacaggcca acgaagggaa aacatgcttt tggctttgct catgattttt
    23761 tcgcactcct tacaagagca ccttgtgcga aacaactgtc cccttagcca ggataactcc
    23821 tcatacagga ggcaatcagc acaagggacc tcagtaagag cagcctttgc agctttctta
    23881 gcttcaaatt tctatcgcag gatacctgag accttgttca ctcatttatc ttttcaggcg
    23941 gaaagttccc attcctctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
    24061 ggttggttgg atacatgcca gtatcgttta ttcaactgtg aaggtccaag tatatgatct
    24121 cctaatatcc ccgacgtgca ctgcatatcg aatcgtatcc ccaacggcat actccacacg
    24181 aggcattcgg aaaagccggt ttggccaatg ggtacgccca ggacgagtgt caagctgaaa
    24241 accctctcca aagtcagcca ggaagtacag acaaaatcca tcaagacctc cagcccgatt
    24301 aacacgtttg ttcgcctgcc aatgcattgg taccgtactg gcatcatcca tcttattcag
    24361 atcaaatgtc ataattggct gaggctcaca aaggaaaaaa tccactgcag cttcttccac
    24421 catgtgtcgg atataaccag gtggttggaa tgccttggct tctggaagat cacgcaagaa
    24481 gctgaagtca accccctgag gaaggtcacg cacggtaaca ggcttctccc agagaaaggg
    24541 cattctgcga cctttcttca gagcaacagg ttccagaaac agttcgaatc gcccaggtaa
    24601 aatctttccg gtgctcttta aagcccgtcg tttcaagtcc agcaggttat gtatcatatt
    24661 ctcgttaaac agatcatcgc ccatttgttc atgtatgatg aagtcaactt tctccggggg
    24721 attgaagcgc tggctgttag aacgtacgaa tgtgatggtg tcgataccat tctttgcggc
    24781 tgttgtccgt gcgatgtcaa tgaaactgga atgatcaatg gcgtaaacct tggctccttg
    24841 tcgtgccgca aaaagtgcaa gaatgcccgt accggttccc agatcaataa ccgtatctcc
    24901 cgccttaaca tgcgcacgga tagcgtcatg ataggtatcg acgcgtaaat ggtcggccaa
    24961 cattttttca tgctggtaaa gactgctaaa ctcccccata ttagaaaaat cataaaacag
    25021 cctgtctaga gctttattag acatgatgac cttctttgcg aggccggtta ttcggctgac
    25081 cattccatca atcatttttc cctccttaga attctcacct ttcgaacacg ctcttaatgc
    25141 actaagatca tgagaagatg cgacaccggc acggtaccca caattatctt tcccacaatt
    25201 tgtgactagc cacagctttg ggcagtgact ttttatcgcc aagtattgct gatcgctgcc
    25261 tgaaatcctt tagctgccaa taggacgttg ctggcaccca gagttggaaa ggaactcatg
    25321 gacgttcacg cttcaaacta gaattgtacg cttcatcaga ccgctccaaa aaacccggga
    25381 caatatatta accatacatg ccacatatcc aacctccccg tagatgcttt ccatggccaa
    25441 aaaccggcga ggctaatggg cctcgtagca ttaattaaat tcagtaatta gcataaaacg
    25501 aatatttgac taagtcgata gtcaggaaga ccactgcaac catagacaga aagcatggga
    25561 ttctcaggga aattttctgc agtatgtttc cttttaacca atatttctga agatgcaaag
    25621 cgcgcggcat tttccctctc cgaacctcaa ggtgaaagac cactttaact atacggagtg
    25681 gaccgcaccc tggtggaatg agccaaactg ttcgacctac atcacgatca ggtgcaggac
    25741 tggaagaagc accaaatcgc caggggggag aacgtatttg gacagggata gccacctctg
    25801 ctacccctaa accgttacag caaaagccgc gtgcaacatc gggcagttgc cgatggaggg
    25861 gtgtgttttg atccgcctcg gacccggcca atgactaggc gcatagccat gaccaagccc
    25921 aagtaaaaac tctcggtgat ccgtcatgtc acctggtggc cgttccatgt tccagtgagg
    25981 tggccacgga taacttgaca tctcttaagt gagagagtcc caaataatta tattccccca
    26041 tggatgtctt gaaatgagcg actgaagaat atgactgccc agttagcatg aactccgatc
    26101 aaggcagcca atttacccgt gaacacttca ctcaaacatg taaagataaa attatttacg
    26161 tgcgcattaa cggaagagga acctgtactg acattgttat tgttgaatga ctgaggtgct
    26221 cagttaattt aaagatctga acttgcacgc ctatgagatc acaattgagt ttacacaaga
    26281 acagaggccg tattgcctta aacaatacca gtagccacta tcaggcccag ggtgacaaac
    26341 acccgagggg gcgtatgtca ccctgttgtc gacaactaaa gtcgagtcgt tccttggacc
    26401 actttccatt taaggaggaa cttggagtgt tcaaataaag tggactatgc tgaacgccca
    26461 cgacaacgtg ggttacgatc caaacccctg agaatctgga atatggagta aaagcgaagg
    26521 aatttgtata ccagcaacga gagctctgca cagaccctgt gaaggtgcgc gaaaattagt
    26581 gagtggtcca tgttcattcg gcacactcta tgcggtccca agtatttgcg atgtgttatt
    26641 cccaattcac tcaattatca ggctcgcctt gacataatta tggtccagcg ataggttgtt
    26701 ttcattggca gttcacgcga tcgaaccggg ttcggcaaaa ggattcacga ggtgtcattg
    26761 aaggtcgtca taattagcta ttgcggcgcc agcgaagcct atgtggccag gaaaatcatt
    26821 gagcaatatc cgtcaaccgt catcattcaa cccgtccgtt cgcagaaagc acccgaggta
    26881 catatcagct ccctcacggg tgcaactcga ttacttcgaa actggctcgg aaagatgggt
    26941 gaaaggcgcc acagggaccg cctgattaat aggctctacc ccggcggaac acagccatcg
    27001 atcccgaaca tcgttccgtt tcctgaatca gatatgaatt ctcgcccggg agttgaactg
    27061 atcgacagcc ttcagcctga tgtactgatg tcctgcggtg cgccaatgct cagcgccgag
    27121 ctgatcggga tccctaagct agcggctatc aatatacatt acggaatccc tcctcattac
    27181 cgcggcaaca acacgctgtt ctggccgatg ctaaagcggg atttcgataa tctcggcgcc
    27241 tgcatacacc atctgacgaa gggtgttgat acggggaacc tgctcgcggt tattcgcccc
    27301 gctctccagc cgaaagacgg gctgctatct gttgaattta agataacgca tcttctgcct
    27361 ggagtccttc tggaattctt ggcactcatc gagcagtctc acgctgttcc ggggataccg
    27421 caaacgggtg ctgggagaaa ttacaagaag aaggagcgta ctatccggaa acggatcgag
    27481 tatcactcga aaaggacctt tggtctattg cgtattccca atcgccctgc agaggtcatt
    27541 aaatactttg gggacatacg gcaatctcag ggggaactcg gccacgagga ccccggatcc
    27601 acgcaggaga aaattgtaat ttaatcgatc tcactccccc ttcctagcca agttaatgac
    27661 agcaatcggt gcctgctccc tgaaatctgg tgagtgaatt ggtggctagt gcggttgaat
    27721 tccagataat tctaggactt cttcggaaag acagctcaag gttctccgct gaaatgcata
    27781 tcttgttgct gtcgcaaaaa catttgcgta gaggggatta ttgcctcgac aacagaaata
    27841 attcatagca gctgttggcg accagtcgca cttgtatgtc ccgtaaccat cacctaagtc
    27901 ataaacttca cagccgagtt cctcaaaacc tctacgaatc gtctctgtta aaagaaggcg
    27961 catgggtgaa taacgggaaa actctggttc aaaaccgcct tgaaagtaaa tataactctt
    28021 tcgatccttt accattaaga cggctgccac tgtggtgtca ttagctcgaa gttggacgat
    28081 ctcaaattga ttccggttcg tgctatcttg catggcccgt tgaagaaatg gaagagtttg
    28141 cccctgcaac aaactggact ggccttttgc gtcaaatcgt cgcaaatgaa gttcaatgag
    28201 ttcactcatg gcaggactga agtcctgcga cttaactacc cggtattcga tcgaatactc
    28261 tttcattagt cgattatgcg ctgaacgaaa agcttttcgc cttgtctgac caaggcgttt
    28321 gctttgatcg gtgatagcgg gatcattcct cagcaataat cgtgggcact gaatctgcgc
    28381 ccaattcttc aggagaagtg cctttgtgaa ggttgactcc cctcccaagt gacggaggtg
    28441 gactatggta gttggaccga gggattcaat gagcgattca gctacagcca gggctagttc
    28501 ctgattttca ggctcacaga gtagtatatc gttgtagatc gacatccggt gggcaaggaa
    28561 ttgcagtgtg gaaattcccg gtatcgtcct tttgtcgaga tacgttggta gaatagctac
    28621 tatctttcca tttgattctt ctgccacccg gacatctaaa ttggcatatt tttgtgattg
    28681 gagaacttcc tcacaccatg cacgattcca ggtccacgat tgaaataccg atggcccatt
    28741 tttagcgttc tgaaattctt cccagattag ctttaagctg tccaattctt tataggtctg
    28801 gattctcttg atcttcaatt tcatgtcaca gtctcctgta atgccctagg gccggcaagt
    28861 accattgcaa gcgaccaata tctatagttg tttgtcgggt ctgctcccta ggcgactaat
    28921 cttacgctga agcttttcaa tgctcttata gtgcctaact tttgaaataa ttttggctct
    28981 tatgaagtaa tggaccattg atttcttttt ttgtgcatac atccagaggt tcgtatatgg
    29041 gcaaaccagc tttgtccact gcagcttata gtcatctgac gcccccataa aatcaatttc
    29101 actgaattta ttggaaataa aatactcgac gttgtacctc attaatattt tccccgggga
    29161 aaactggcga taagcttggt cgtagccaga cttgagttcg tacacggtac ctccaaaaca
    29221 tatattgtat tcaaaggcta tgggatggtc atcgagaaat agaattttga gattcaacca
    29281 cccacgatca gccgccgctt tcgcaaagga agcataaaat cgaccgtcct tgcctgagga
    29341 aatatcagtg ccctcttgag cctgccagct ccgttcggat atctcataaa taatttcaat
    29401 tcctacgtct accctatcag gggttgaata atctaacgtc ttgagcgttc cgcacttctg
    29461 aatatccttg atacccctac gtatagaatt tcgaagattt tttgaaagat ttttgtagta
    29521 attctcccag tcagttttaa taggcaagaa gggtgattca atactggtat cccatatcca
    29581 tctacgtgga tttttcttca aaaaatcctc taataaatgc attgtcgttg actgtttctt
    29641 caagccccgc aatttcatga catcccattc gttttccgcc tcataaaggt acgagaagaa
    29701 tgccgcaaca caccgggatt gatcagactg accaatgatt atttcattgg gattagtgtc
    29761 agatacaaag cctagctgaa caaacgacat cccatagtgc ctgactacag ttcgtacaag
    29821 cggtgccaga ccgatgggct ttccattttc cagaaatagg ataatgaaag gactaatcgc
    29881 ttcggaagac gcgtagtacc atgagctaaa ccaatcatat gtggcccagg gaggacagct
    29941 agtgttggtt ctcgctacct cgtcccagac cgggcccaag cggtccaaat cttgactcga
    30001 tctcactacc tgtatgttca ctggcatcat ccttttaaat tagaccacga gtatatgaac
    30061 acaactttct tacgatcctt tatggacgct tgctccccac ttcaaattct gctcgtgaac
    30121 agatggagtt tcattcgaac accccttcgg cctaaaagtg tactctttat tcaacgccgg
    30181 accaccggca tggtcgagaa tttttatgtt catagggcac atgtctcact taccttctct
    30241 agttctgccc ggttttttag cttgttattt ggtaagacca ctctgtattg gacgattatg
    30301 cgataaatag ttgagaagca gccgaagttc atcctgtcga atatcgctaa aaagaaccaa
    30361 cacgccacta taaatgagga cagcacaaag tatcgtggca gacagtggca aactgaacca
    30421 aaacactaag atcatcagaa aaacggcgac cgcagtgcta ctaacaacaa ttctgatacc
    30481 gacgttctgc cacacaaaaa acttcttaag ctgaaagcca agaagcccaa tgagcaaaag
    30541 ttccgccaag agcgttgcta ccgctgcccc ttgcaaacca aacctgctaa taagccagta
    30601 gtgaagaatg cccccgacac acgcaacaag aaattggctt ctggtgacgg cctcctggcg
    30661 atcactggct atgagaaaaa cccgcatgat acacctgaga aataccaccg ggacgagcca
    30721 ggcaagccaa cggagtatga caattgattc aacaaactct aatccataaa gaagctcgat
    30781 caatcttggt gcaatcagcc aaatcccccc cgccattgga agaccaagaa caatagccac
    30841 gtttagcgtg actcgataga gttcctgaaa ctcctccaac gaactgacat aaagacggga
    30901 tgcaagcggt aaaattgcga ggccggcgaa tatcggcata aacaacagga ccacaataaa
    30961 tcgataggcg acattaaaga ccccggtggc ggatacagta agcatgaacc cgacaaataa
    31021 taaggcaacc ctggaggaga atagatccaa cagggaatac agggcatagg ggaacgcttc
    31081 gcgcaacacc tgacgaacac cggcagtcct aacggaaata cgaagcgccc cacaacgcct
    31141 gagaattaag tgatacccaa gtgccacatg aagtaaggtc atgactggca gggatgatac
    31201 ggcgagtaac aggcttccac ccagataaac aatcaccatc cctgttaaag ctgtcgtgat
    31261 tcgaagggag acctcaaaaa gactcgtgat gtgcatatcc tcccaggcga caaatacagc
    31321 tccaaatccc tcaaccagtg tgagaaaaat ttgataggct ccgatcacaa tgatgagaag
    31381 cttcatctca tccgggaaag ggtacagggc cgtcatcccc agcagcagag atataaccat
    31441 tcccgatagc aagagacgaa ccgccagtag gcggccatag taggtcgtta cctgatccct
    31501 gtaacggctc agattcttta tggacagggt ttggatcccg aaatcggcaa aaacggaaag
    31561 tacacccgtc aaggccatgg caaacgaata ctgcccgatg ccttcttggc cataggttcg
    31621 agccagaaca aggaagaaca gaaatgtgca tccatcagca aggagcttcc cgatggttaa
    31681 atacccgaaa ttcctgaata tccttgctcc tgccccaaca tcgttattgg catcttcatt
    31741 catatgacag caaatctctc atgggaacaa atggttcttt cttaaaattg ggcaacgtaa
    31801 catctctacc cttcctcgtt accccatcta tgtgacaacc acccactaca ttgctccacc
    31861 gaataacctc cataatggcc tacgtccaac gcctggaagc ctatagatat acttcggttg
    31921 aagccgaggg attgggagcg gttggatctc atccttatcc acgaattgcg gcaggagact
    31981 cccccaagcg ttggtaacgt atccggcctc aagggaaagc tccgctgcaa gtgcagaccc
    32041 cctgtaccag ggaaaacaaa aatgccgcac ccggctacct ggcaggttgg cttcaatggc
    32101 tgccttcgac cgacacagat ccgtgaggat agcgtttcgt tgttcatcgg cagtttcgaa
    32161 ttcaattgga gcgcattttc cctgtgcatt ctttatgatt tgtttgagtt gacgccgcca
    32221 gtcccgatcg taaaagaatt cttgaccgcc gtgatctgcc acgtatctga cacacaggtc
    32281 ttcgatttga acgttttcga taaacgctgg attcgcacac atacgtgcat tccattgata
    32341 tatcggcgat ccccaaaagt tatgaagttc ggtgcgagaa ctatccaaag tttgctgatg
    32401 aatcggcgca agatctgact ccagaaacga ggtggaaagg cctggtcttt tgaaatccac
    32461 taccgtcggg gagatggcga tactgtggtg atacagagaa tgtgactgga aatctatgac
    32521 accacttgaa tgcatttcgc gtatctgtgg ccaactgcaa agagcactat ctaaatcgtt
    32581 tccagacaga ttcgttacgc catctggcac tcgcccaggg acgatatagg ccaccgccct
    32641 ttgcttgaac ttatcgagca agggaaaggc gacggtatag aggctggctt gaccatcatc
    32701 gaacgtcagc aacacttcac gatccccacg tggcgaggcc ccgactgtcc gttcgtaata
    32761 ctcatctccc gtcagggtga catagtcatt tcgggcaata aattccaaga tcggttggag
    32821 ggtggccgtt gaaacgtcgt ggaactgaaa gacagggatc tgttcgggtt gcacatccga
    32881 acgcacaaat agcggatacc gtccgagccc caatccaagc agctcgttac ggttcttctc
    32941 aaagcttctc ttgaaatgtc gaatagtcaa tgttgcacct taggcaggag caacgaccca
    33001 ctcaaaggca tccagcgtgg cttttgccgt tttctcccaa gaaaaccgtg ccgcgttttc
    33061 atatcccttg gcaataagtg tttgccgcag gtcgtcatcg gtcaagaccc gaccaatggc
    33121 atttgcaatt tccgcaacgt ccagtggatc gacatagata gcggcatttc ctgccgcttc
    33181 aggacttcct cctgtacgac tggtgactaa cgggcatccg cacgcattcg cctctatgtt
    33241 tggcaggcca aagctttcgt agtgtgaagg aaacactgtt gcagcagcga ggttgtaaaa
    33301 cgcgacaagt tcatgttgcg gaacataccc ggcgaaatga acgcagtctt gtaatcccaa
    33361 gctttctaga gttttcatgt cttcttgata cttccaacgc ataccgccgg ccacgacaag
    33421 atgaacatcg ttatgttttc ttaccttcga aaaagcctcc aaaaggcgcg tgaaattctt
    33481 cccaggatag atatggccgg caaacagaat aaatcgcgat ggtagtttgt atttaactct
    33541 gaccgggtcc agctgtcttg gatcagtaat cgatcgaaag ataggatcag tggcaaggta
    33601 cgccagcttc actttatccg gatcaatacc caagtaggaa ataatatcct ccttcgctcg
    33661 attggatacc gcaatgataa gggaagcctg ctttaaatac tggggataga cggttttaaa
    33721 aaaccagaga tccgcagtct gatgaaactc agggtggaca aaccgttcag tcccatggag
    33781 taccatggct gttttacatc gcgccagaaa cgggatggca aacttggtgt gaaagatcag
    33841 gtcgagaccc tcacgatgcg catacctcgg cactgcccat tgatcccaca tccacttgcc
    33901 tttggcagac aagtacacct cccggacgcg atccgtgcca ggaagcgatc caaggtgtgc
    33961 cttgttggag taaagaatgt gatactcgtt tcgagtatcc aaatgtaaaa tggcctcaat
    34021 cacgcttcgc gtatagacac caatgccgcc attttctccc catgttctgg cacaaatgcc
    34081 tattttcatg aggattcctc tccaggtaac gtcaaaccat gcttaaggaa cagggcctca
    34141 atttgtcgcg atgaggaaag gtctgcggct tgttcgtcct caagactcac acctaacatt
    34201 tcttcaagct ccatgatcag acgcagatga ttgatggaat cccagtcgga gaattccctg
    34261 cggtgcagat cgtcgatatc cggaggaatc tccacctgaa gcactcctga aagaaactcg
    34321 atcaccttcg catgtgtgtt tgacataatg ttcgtcctta tccattccgt gacactttgt
    34381 gcaacgctgg tggtgactct gccttcagct cccgatgcag catcttgcca agactgtttc
    34441 tcggcaaggc atcaaccgcc tccataaaaa acgggacctt gtgactcgga agagacctgg
    34501 cacaatggcg ggtcacggct ttaagatccc cagtcgcgcg gtcccgaaaa accacatagg
    34561 ccttggcaac ctggccaagg acacgatggg gcacaccaag caccgccacc tcggcaacat
    34621 ccttgagctc cagtatgcat tgttccactt ctacgggggc atacttccta cccccacagt
    34681 taatgacatc aactttacgt ccatgaaagt aaaaaagccc tagggaatcc ttgtggccca
    34741 tgtcaccggt tttcaaccaa cctccgggtg caaacaacgc ttccgtttcc gccggaagtc
    34801 gatggtaacc aaggcacgca tgcggacctc gaacccaaat ctcgcccgtc agtccctcct
    34861 ccacagggca cccctcgtcg tccataactg ccatctccac accgggtatc ggaacaccga
    34921 tggaatccgg atgttccagt ccgacggtat ctgagagtat cgtgcaggta gaggcttcgg
    34981 tcagcccgta cgaattgatc aactccacgt tggggaaccg ctccttgatg gccaaatggg
    35041 tttgagactc gatcgccgcg ctggaattca tcaacaaacg caaagaggga agatagccgg
    35101 catgaaactc tggatagacc attaacattt tgaaggtggc cggcaccgca cggaagcagg
    35161 tgacgttcca cttacgaatt cgtgccagaa gatcaaccgc atcaaaacgt tctaccaaca
    35221 cggctgttcc tcccacttca atcatgggca acatctgact aaccagggca ctggaatggc
    35281 tgagtggcat aacgatggca atgacatcct catgccggta gccaaatgcc cgacgtgcgt
    35341 taccggatgc agctctggca ttgccatggg accgcatggc gcccttcggc ttgcctatcg
    35401 tacccgaggt atacatgaac tcggcgatgg cgtcaggcgc tggtgaaatc cccgattcat
    35461 ggagatcccc gggatcggcc ggcaggttca accacccatc gaaacattca acatttgaat
    35521 gtgtttcaac cttgccaata gacaatacat gttcgagggc gggaagtgtc ttccactccg
    35581 tcgaaaattt ggaccagagc gcctgatgaa caatcgcgca ccgggcaccg gagtcttcaa
    35641 gaagaaagcc gatctcctca ggcttcagac gctcatcgac aggatgaaca atcccggcaa
    35701 tcgcggtcac cgcaaagtaa ctcacgacga actcaacgct gttcggcgcc agcaaagcca
    35761 ccacgtggcc cggccgaacg tcccattgac gaatgagtgc gttggataca cgacgtatac
    35821 gccgagccaa ttcacgatag gtcagttctt cttccgcagt gatgagagca atcttatccg
    35881 gccacagacc ttccgcctct tgccagacta tgggaagact cttcatgctc cgttgacctg
    35941 ctttcgtttc tcgacgactc gggcggggtt acccaacacc acggtccatg gggggacatc
    36001 cttggagacg acggcgcccg caccgacaat gctatttttc ccaatcgtca cgccatcaag
    36061 aatctttgcg ccaatgccaa tccaggcacc gtcttcaacg gtaatcccct tcgatgtccg
    36121 ggcttgctga atgaccggca catcggggtc atcatgcgca tggccgccac caagcatgtg
    36181 cgttcctgct ccgatcgcga catttgaccc tatcgtcaaa tgactgcgtg aaaccaaaaa
    36241 acaaaatggg ccaatcgaca caaagttgcc gatggacatc gaagagtcat tgcaactgag
    36301 aatactattc cggcccagga ggatctgatc gccaagcacg atggaagagc cgtgtccttt
    36361 ggcatcaaga accacgcaat catcaatcat cactccccgc cccaggtgaa ttcttcccgg
    36421 acaacggagc acgccccctg caccaaagac cgtccctcga cccaccgacc ccagtagccc
    36481 cgggtaacac ttgccccgta cgaaataccc gatggcaccg ggtaatcgac ccagcaccga
    36541 gatatacagt tcatatttgg ccagcgcacc aagagattcc tcgccaacga acatgctgag
    36601 gtattgacga tacggagaaa gttttgcgtc cccaagcgac tcgactaacg gtctctcatt
    36661 catcccggtg ctcccttgcc gattgcaaac agatacatag tgtttagaga tccccgtaga
    36721 ttcttgatat ggaccatggg caacgtttag aggagtccat gatcctcata ccaacggatg
    36781 gttcgtttca gcccttcctc taaggatacg cgtggagcat agccaagcac gcgtttgact
    36841 ttttcgatag aaaaagcccg attattttta aaaaagctca cccgtcgttt gtgcagagga
    36901 ggttctattc ccagtggggt gcagaccgac tcacagagac cggcaagcca ttccacgggc
    36961 ctaagcggaa tatgcatact cggggcagga acctgaagtt gcgcggaaat gcgatccacg
    37021 aggtcatgca agggaaggta tccatctcct ccgatgatga acgcttcacc tacggccttc
    37081 tcatgtgatg cacaaagcag aaacccttca atcacatcat caatataggc gggatgaaac
    37141 aatacattgc cttgcccgat atagataaac tgtcgctttt ttatggtgcg aaacagtttc
    37201 agcattcgaa gatcttccgg cccatacaga gaaatcggtc gaacgacgct aatgggcaac
    37261 cctgtttcct gatgaaattt ccacacgtac tgttccgcca ggagcttcgt ttcctgatat
    37321 aaatccgtgg ggttgaacgg ggtttcttca tgagccggaa tcgactgcac atccccatgg
    37381 acgccgatcg tactgcaatg caccacccga cccaccccat atttcttggc ggcaccgagc
    37441 acattctttg tcccttcgac attcacggca taggcatcgg tcttatccga tccccctttt
    37501 cgaaagttgg agaccaagtg aaatattttc tccactcctt tcacggcctg ctccacatcc
    37561 gggagattac ggatgtcccc ccatatgact tcacactccg taatggctgg cgaggcctca
    37621 ccctgcttgc tcccccgatg cgcaaagacc cgaacctcct gaccgtctgc gagtaaccgc
    37681 ctgacaagat gggtccccaa aaaccctttt ccgcccgtga ccatgactct tcccattacg
    37741 cgtttgcatc ctttttgttt tgagcaacca ctaaaagcat gtatccactc gtaaaaaata
    37801 aggcggtatc aagtttcgag atcgtccaaa taatcgggta gagcaatgaa tacatggtga
    37861 acattttttg atattgcttc agatcttttc cggtcacgac cagccccttc gcagaagtcc
    37921 gcttccctct cttcaggaga tcaaacgcaa acgtgatcaa cgtatcgata cattctgaaa
    37981 agaatttgga ataggtcttc tccgatacca cctggaaatg atcgcccagt atccgattga
    38041 tgtcaccgcg ggtatatccc gggcggagat gcccatgttt ttcatcagtc tggccaatgg
    38101 ccaatcgaat tttcctgagg agagtcggtt ttatgtgcgg cacattcaga acgactagtc
    38161 cataaggctt cacaatccga tgcagctcac ggataaaggc ctcatcattg tgaatatgtt
    38221 caaggaaatc gatcacggcc acacggtcga attcattgtc ggcaaatggt gttggacgat
    38281 catcaatctg aaacaccggc tcctgaatca aatcttgaat tgtttccaca gtggtttgat
    38341 ccaagtccgc acttttccaa tgtcctcccc gttgacgaag cagatagctg atcacgccat
    38401 tatccgcgcc aatatccaga cactgtaatg tcgtcgtttc gccaagtgct tcggtgagtt
    38461 ctttgtattt ccgttgtttc aagacggatt ttttaaagag tcgtaagggc catatgggct
    38521 ctgcgtgttc cgtcatgttc atcccggata ccgacctcgc accatggcca actcctctag
    38581 ggtccggcat tcactccatc ccaactcttg ggaaagaata tccgcacaca aatccagggg
    38641 catatcgcct ggataaccag ccgtccctaa ctctgttcgg cgaaaaacca catcacttaa
    38701 tcgctgggcc atttcctccc gcaaggcata ccggacttct gcttggagaa tctccataga
    38761 ttctgacccg tccgaggtgt ttctctcgcc cgaatcaggc aaatactgaa gcaccgtgcg
    38821 ataagaagac ccataattgt gaatcaagcg atgtacagtt ttttggctca atttccctgg
    38881 tggacatttc atttcctgat ttaaaaattc ctgaaatctg ccaatatttc ccccataaag
    38941 aggggttata gccgtttgtg aaggtgatgg agttctgccc aacaaccgaa acacccgatc
    39001 aaccacatgc tcggccactc ttcgtgccgt tgtgaatttc acccccacca gcgagataac
    39061 atttcgcgca cccacatctt cccttgtacg gatgtaaaca tcactttcca gttggatatc
    39121 tctgcggcca gactccgcct tcgctccagg gagcaatccg ccattgacca tggccacgtc
    39181 ttccatcgtg agagccgcgt ggggatagga ctgattaatc tcctgcaaaa attcatgaat
    39241 atcttcctcg gaaatcctga actcatccgg cttgccttca taggagcgat aggatgtccc
    39301 gatgagggaa caatcccgcc agggcaccat aaagagcaat cgtttcccct ttttcagcag
    39361 agctcggtca tcggaataga cttctctggc cgccagcccg accgcatagg aagagaacaa
    39421 tggacgggta ataatattaa aagccttgac ccacgggatc ttgtggtatc cgggtcgatc
    39481 agacaacatg tcttccgttt gatccaacca gggtccgcac gcattcacca ccacgttggc
    39541 ttcgacttga aagcgctccc ctgagagctg atcctcagct tctaccccac gaatctgatc
    39601 cttcgttttg agatatccca ccaccttgac ataattcgcc acttccgccc ccattgattc
    39661 ggccgaatgg agaaatgaca gaagcaatcg ctctgagttg tacaccatac cgtcatgaaa
    39721 cactccacca cccgtcagat catgttcagg aatattcggc aacagctcca gtacctgacg
    39781 tcgagaaaga attcgtcctc gtggaatatg tttttgcggg tcgtccaatt ggtttcgatc
    39841 aaacccgata agatcgttga ttgctaaggc acatgcaaaa acctcttttc ctcgcaggcc
    39901 atgcccatag gtgggaataa ggacaggcaa gggatgaacc aaatgggggg caacccgcat
    39961 aagagccatt tgctcctgca tggattcccg cattcgcgta acatccccat gctgtagata
    40021 cctaaatcct ccatgaatga tctttaagct attggccgat gtccctgaac cgatatcccc
    40081 ttgctcaaac agcacgaccg ataatccacg aagactcgca tcccaggcca cacaggcacc
    40141 ataaattcct ccgcctacca ccgcgacatc gtaccgggcg ttcgctaaac gaggtaaatt
    40201 tctggtcaac atcgcagcta aaataaatag aaagtccgac ctaagttata gattcggagg
    40261 gctgttgtat ggtcgtcacg agatgatcat aggccgcatt caatttcatg cgatatgcgg
    40321 gataactgta tttctcctca accagtttct gcgcggcctg cccgagagac actcgcagtg
    40381 gccggttccg aataagctga agcaacccat cagcaaataa ttccggagtc gggtcggcca
    40441 acatggccac atcccgattg ataacctggg tatgggtcat caaatttgtg gcgaggacgg
    40501 cctttccact ggcgagatag gaatacaatt tcatcggagt attattgcct ttgattcttg
    40561 gcga
//
LOCUS       DAORAQ010000009        39669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_219_length_39669_cov_66.376180, whole genome shotgun sequence.
ACCESSION   DAORAQ010000009 DAORAQ010000000
VERSION     DAORAQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 39669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39669
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219_length_39669_cov_66.376180"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1794
                     /locus_tag="PKM72_01785"
     CDS             313..1794
                     /locus_tag="PKM72_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP59537.1"
                     /translation="MEENTEYHAELFPYLQSWGLREFRDEASYYGWQRETLSKEELHD
                     FQRLIEHRYGEENGKGDIEFYDGLARRDLIPILYSQRLQYFLTIGTAVCRRIAPAQQV
                     LDYGCGVGILTIFFALQHPDVEFVGIDRSPRSVEMACLEAEKRQIRNVRFEISHVPPQ
                     QISGTYDLILSTHTLFQAEREPGLPSRTWETFQRANDPQRQEQLEVLTGVQGRLDALM
                     LVLGSIGRMLLFEKTWNLGRRIFFQRALGARGLFPISSPVFCRYRSVDDEVTDGPLYE
                     VARITQGVETHGWNEEPYRASGETLYRCIGIAAERMGRVLATDQVSASVSGVHSSMGS
                     WMFRFGLWKEIFAWGLCQYSSGLTGLVIGAGADRDLLHQLVETVTYITESDFQQLVHD
                     FWGGMIHAGEDQSAPCYENHHSSAQIIYEGLPSKHVQRESTFQDGERGEMHIELGTTT
                     HLTYLYFANVFDQRQLVLMDEGRAEILSEHYRESLQHRHGPPS"
     gene            complement(1826..2140)
                     /locus_tag="PKM72_01790"
     CDS             complement(1826..2140)
                     /locus_tag="PKM72_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59538.1"
                     /translation="MRLAIIFFLSLLFWSVTISIEYGNRNTNTTFTAQACSGEVNGDV
                     LLDYLQIGRIKGIRDNGRILTVGLTSDWATLPSDIQQKTYEAVACYAKTQQRTLQVIE
                     TP"
     gene            2514..3479
                     /locus_tag="PKM72_01795"
     CDS             2514..3479
                     /locus_tag="PKM72_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59539.1"
                     /translation="MNGETLLFWVLLATCLTHWLTVVLLLRRVRSTENTLVWNLLSVA
                     VGLLAIQKTYGITIPYLELNPPPVNFVSTWLGFVVAGLLLAGIMRLGPFLYLLERNKE
                     LLAVIEERNVIILHFYERIARALQKIQMAMEVGRPTNCIIDQVAEMSEMLKGFLENLK
                     AGVLLGNKFEVALKTLVEDLSQEASFPILVNVDPSCEDQLSREQGAQLLHIVREAVLN
                     SIQYSHAKKGQVSAKMTATDTVVEVSDNGKGFEVDLVRAQGHGLGNMVNRAKKIGARL
                     KIHSQPSKGTSVFIEVPLNEAPAENRHSASTVDSSSNATQRAPVG"
     gene            complement(3560..3635)
                     /locus_tag="PKM72_01800"
     tRNA            complement(3560..3635)
                     /locus_tag="PKM72_01800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3600..3602),aa:Met,seq:cat)
     gene            3842..5086
                     /locus_tag="PKM72_01805"
     CDS             3842..5086
                     /locus_tag="PKM72_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP59540.1"
                     /translation="MTLTAAPNIKHSVLVGRQAIFDREKGVFGYELLYRDGEANSAQV
                     IDGDEATARVMVNTFLEMGIDQIAGTGQAFINLTANFFLSQNYEVLPKNNVVLEVLES
                     IEPTPLVLQSLVRARQLGYKIALDDFIVRDSHRALLEVTDFVKVDILALTSEQLLEQI
                     DVLKQYPVRLLAEKVENQEVYTFCYEQGFEYFQGYFFCKPQIMEGVKLSGNRMAIVLL
                     LAKLQDPDIKMEDLEALVENDLSLSLKLLRFVNSASVGLPRVVNSIGQAVGMVGTERM
                     RQWASLLVLANTGGKPSELMRIALIRGRMCQGLSRLQGESPSQGFTIGLFSVLDAYFD
                     CEMKQLLAGLPLASEVLLALTERQGCFGEILTCVMSYERGDWDQIEDSRFEPHVIRHE
                     YFLSTEWANDVMKATLGAGDGT"
     gene            complement(5123..6289)
                     /locus_tag="PKM72_01810"
     CDS             complement(5123..6289)
                     /locus_tag="PKM72_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59541.1"
                     /translation="MPTTLRFEHIESAIQTLPTQGRTMLQLLLLQYLDLSPESIAYMV
                     NDQPDSRFQAGNQPKGNPLSLEAERNITSRANQYRDYYRQKRERPGMHIEFLTQELKI
                     LDETIQIAERLLTSEFGLTQNGLQDAKTQAPSILLRQELRKLDRGWDNQELSTKEYQA
                     QRLVLEYQSLLRRRGIFRRRLKFAQNDFIVSGNSPLQDHEIAHIWGIPLGSLVARKVK
                     ALQQFLTELQKHQDKHSAPDEPLQPINLWKEALDFLSQRPFEKSMVEYDGLGKTEEAL
                     LDQLRAFVEGSMSEPEESKFWTSITKVNDTEFSGTWLSQARSILAFQRLNALLNDMDF
                     SDEGLEESLRVKIYPQLPDDQLAPESDDKPIELSEKGVGVLNYFAGEQDDKRRG"
     gene            complement(6341..6556)
                     /locus_tag="PKM72_01815"
     CDS             complement(6341..6556)
                     /locus_tag="PKM72_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59542.1"
                     /translation="MEGEKDVLFVRRDKDGAVTLFIDEDWAAERGIDPSQLVKIEIPR
                     ELYANGTVQQLREYAATCLESLDNGTP"
     gene            complement(6960..7673)
                     /locus_tag="PKM72_01820"
     CDS             complement(6960..7673)
                     /locus_tag="PKM72_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNP59543.1"
                     /translation="MNMAGAEEAAKQQFVNFAFYKVDPLWRRLPKEVRDQGKQEFVRA
                     AEEYTGKVIVVPYTTTGIRGDCDFLLWRISYDLEMFQEMSSKLLATGLGQYLTTPYSY
                     LSMTKRSIYVDHHVHEGQESRRLKISPGRAKYIFVYPFEKTREWYLLTKAARQGMMDE
                     HIAIGHKYPSVKLNTTYSFGLDDQEFVVAFETDSPQDFLDLVMELRSTEGSRYVKRDI
                     PIFTCIMKSLKEVVETVGG"
     gene            7931..8512
                     /locus_tag="PKM72_01825"
     CDS             7931..8512
                     /locus_tag="PKM72_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59544.1"
                     /translation="MMLFEVGLVILASSPVWGIVSDPSQSQILEAVHIGQEGASSRTP
                     PNMLYRHFGGSSSDVPQAQGFLMTKLNGIAVLSCHFALRGENPGSEDIQRVLDEKALQ
                     VVVAIFGDSPTFANNSYLLLKQENRLIKPDHIRFDARASQISQSQELPIFRAKIVASF
                     NYDAFDALAQTTIKVFPGAGGEVTFDLDFADIP"
     gene            8568..9848
                     /locus_tag="PKM72_01830"
     CDS             8568..9848
                     /locus_tag="PKM72_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNP59545.1"
                     /translation="MKLFHAEIIAVGSELLLGGRIDSNSVFVGHLLAECGIEVRKKIS
                     VGDQKGEIQEALRGALKRAHVIVLTGGLGSTLDDCTREAVAETFRCPLIKRKRAYDNL
                     KACYRRFGRPVTPILAKQAFLPSGATMLANTVGTASGFLMRQGQTVIVVLPGVPREAK
                     VMMIDQVQPILRKIFKSKRHYWLHTFHTFGLPETDVQKALSAVLREDGPIQFGLLASP
                     RGVTVTVSCWMVSDSQSFAKERSPFFPEWADLVRQVRECIGPWLFAEGERTMEEIVGE
                     ALRGLSWTVAVAESCTGGLVAHRLTGVPGSSLYVDRGVVSYSNEAKQELVGVPASTLR
                     RYGAVSSQVALAMAKGIRRRSRVNVGVGVTGIAGPGGGSLDKPVGLVYGAIDGPQGPR
                     WYRWQFHGDRAEITLKTSQAVLDLIRRYANEASF"
     gene            9832..10449
                     /gene="thpR"
                     /locus_tag="PKM72_01835"
     CDS             9832..10449
                     /gene="thpR"
                     /locus_tag="PKM72_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNP59546.1"
                     /translation="MKLHSNRNSLRVFLAVELSLDLCRKVREFQQQLQNFLPHVNWVR
                     PESIHLTLKFLGNVDASMVDQVLTALEPIGKSQPPFTLEIQGLGVFPHVRRPRIFWIG
                     CTGDIPALLNLVSGVEGALDPLEFPSEEKPYVPHLTLARIKHDNSQVGKVLTHSGLLE
                     QPQNLGILPVARITLFRSNVTTIGVEYIPLWAVPLINEAVSNFSS"
     gene            10533..11633
                     /gene="recA"
                     /locus_tag="PKM72_01840"
     CDS             10533..11633
                     /gene="recA"
                     /locus_tag="PKM72_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNP59547.1"
                     /translation="MAERVTPQKETQKDGKKRALDLALTQIEKQFGKGAIMKLGTDNV
                     PHDIPVISTGSLGLDMALGIGGLPRGRVIEVFGPESSGKTTLCLHAIAEAQKAGGAAA
                     FVDAEHALDISYARKLGVQTDDLLVAQPDTGEQALEITETLVRSGALDIIVVDSVAAL
                     VPRAEIEGEMGDSHMGLQARLMSQALRKLTGAISKSQASVVFINQIRMKIGVMFGSPE
                     TTTGGNALKFYSSVRLDIRRIESIKDGQDVMGSRVRVKVVKNKMAPPFKQAEFDVMFA
                     EGISKVGELVDLGVERRIVDKAGAWYSYKEERLGQGREAVKTFLKSNPDIAKDIDTQL
                     RNSYGLSVEAQQKEESSAPASSSGKKSTGDRD"
     gene            11676..14336
                     /gene="alaS"
                     /locus_tag="PKM72_01845"
     CDS             11676..14336
                     /gene="alaS"
                     /locus_tag="PKM72_01845"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNP59548.1"
                     /translation="MIATSLELRQAFVSYFSTRGHTVVPSGPLIPQADPTLLFTNAGM
                     NQFKGVFLGEESRPYSRAVSIQKCMRAGGKHNDLENVGFTRRHHTFFEMLGNFSFGDY
                     FKEEAIAFGWEFLTQIAGLPADRMWVTVFRDDQEAYDLWHTRMGISESRLVRLGEKDN
                     FWQMGETGPCGPCSEILIDQGEAFGCGRPTCAVGCDCDRYLEIWNLVFMQFDRDLAGT
                     LHPLPKPSIDTGMGLERLAAVTQGVSSNYDSDVFLPILERIGKATSQEYGASLAADRS
                     MRVIADHLRAMTFLITDGVLPSNEGRGYVLRRIMRRASRHGRLLGMEREFLYDLVPSV
                     VDRMDSVYPDLRSMKDTVCEIVQGEEARFIGTLEQALPLLNQILTEAKQKGSQILDGE
                     AVFKLYDTYGFPLDLVEDAAREEGLAIDHDGYQQALEAQRDRARKTARFSQGDSRSAL
                     VSALEQFPLTQFVGYAQQEGRGRLLALVQDEHVIKEAKQGETVECILDATPFYPEGGG
                     QVGDQGALVGPSARIAVEDTTKLAKGWFLHKGRVIEGSVHVGDVLIATVQGQLRQNAA
                     RNHTATHLLHAALREILGPHVKQHGSLVAPNRLRFDFSHFKGLTPRNIEEIEGLVNEQ
                     IRQNLPVMVEEMGIQDALSRGALAFFGDKYGERVRVVEMGSFSQELCGGTHCVRTGDV
                     GLFRILSEGGIAAGVRRIEALTGEGAVLQTQHQEAEWRELGAVLKAGPNEVVEKAKKL
                     VGTLRETERELERTKQKLLDQQGAGQETLIRNIGGMPILIQRIDGLTIQELRIFSDKL
                     RHKVPSGLLVLGSVKEGKVSLLVIVGKDQTHKIPAGKVAQHVAQLVGGSGGGRPDMAQ
                     AGGNQPEQLDAALQSVYDYVASQIGALTEE"
     gene            14418..15425
                     /gene="mltG"
                     /locus_tag="PKM72_01850"
     CDS             14418..15425
                     /gene="mltG"
                     /locus_tag="PKM72_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNP59549.1"
                     /translation="MILVCRRIVSLLSGVVLVMVGFMWLNQPVGGPAQPVLVEIPPGT
                     PFTQVSDILDQHHLIKSGWFFTFLGRVQRVDRNIIPGEYELDAGMQPTELLNKLVKGK
                     VYQYAITIPEGFTVAQIADILDYKRLALKQDIFRLSRDPVFIQSLNVHAPTLEGYLFP
                     DTYHFSRFTPPETIIRTFVNRFHDVLTPELKARTTAMGMTLQEVLTLASVVEKETGLA
                     AERPLVSGVFHNRLRLGIPLQSDPTVIYALESFDGNIRKTDLSVDSPYNTYKVRGLPP
                     GPIANPGLAAIHAALYPTQTDFVYFVSRNDGSHQFSVTLADHNKAVDIYQRRLIGKRA
                     S"
     gene            15446..15922
                     /locus_tag="PKM72_01855"
     CDS             15446..15922
                     /locus_tag="PKM72_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNP59550.1"
                     /translation="MGIHETLSRLGLTLPEAPCPVGSYVPARQAGDLIFVSGVLPFRD
                     GKIEQPGKLGRELTVKEGAAAAQLAMLNGLAILQEMMGDFSCLNQVVRLTGHVASVEG
                     FVDQPTVINGASDLLVKLFGDTGRHARLALGAFELPLHAPIELELIVQVFPPKAGP"
     gene            16423..18078
                     /locus_tag="PKM72_01860"
     CDS             16423..18078
                     /locus_tag="PKM72_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNP59551.1"
                     /translation="MKRVRILMSVALGFLLFSSIAHAEMFPSDGPPGTTVTISGEGFG
                     EFRNTQANRVEFQGVSALIQSWEPDFILVKVPLQARSGPVMVINGSAKREAGIFSVTD
                     VRISSLNPSQAEAGSLLTIVGEHFGNTAGSRDPNTMFGVNQVIINGIRAEVRRWRPTK
                     IEVLIPANAKTGDVEVRLASSDPLPDGSCCAPVEYAVSNAVHLTVIPPIAFDPTEGPI
                     GSKVVLSGQGFGESRPEDGRIFFGGKPAIIAQWSDRTIVAHVPLNAQSGFLMLQREGK
                     EREIGTFTVLDSQVSGMNPAQGPIGTLVTIKGKNFGLYSEAGSTAYAFDFLSGGNGVE
                     IGGVPAVIHRWLDEQIDVWVPYSAKSGPVIVKRGGAIPKTDGTCCEQQEIVTLKAGTF
                     TVVQPEIHSYSPKEAGLDEVVTITGKGFGEFLKISEATRLSLNQYAHDWKIYELGQDV
                     SRSEVLVNGVATHIETWTDTEITIRVPRRPSYGFGNPKGFEADLSKGELILKRGSWDM
                     LDSGECCTPKKYISIVAGPFTILQRGLPNKGYWNEKNGERSND"
     gene            18140..19114
                     /locus_tag="PKM72_01865"
     CDS             18140..19114
                     /locus_tag="PKM72_01865"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNP59552.1"
                     /translation="MKSLCALGVFASLGFFVNLQPAFPQSPPSALSLQSSPTKVTFLG
                     VHFLTASKGWVVGAGGTVLHTDDGGASWKSNPRRTNALLTAVTFADAMHGWILGQNGT
                     VLHTVDGGKQWIPQDSGTNGTLYAADFLTTDHGWVVGSRGVILHTEDGGESWADQVSG
                     TTADLFGVHFLNEKRGWAVGALGVILATTDGGKSWGLQSTNNPATFFDIVFTDNVSGW
                     AVGTQGAIFQTLNGGEVWVDHTRPCGSPCIKPADLVNIQFLNSLVGRIVGERGTILET
                     QDAGFTWQEIEPLNSETLYAQSFPDLSHGFAVGDHGTIIHFETASPRP"
     gene            complement(19139..19873)
                     /locus_tag="PKM72_01870"
     CDS             complement(19139..19873)
                     /locus_tag="PKM72_01870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNP59553.1"
                     /translation="MPVYFIHSTQVEDERVIIGNPLFTHLSKSLRTRPGDRLILNDDR
                     GRRYQTTIHQITKQAIYANVLSIQESSPSSTPPITLAQALLKGEKMAWVIQKATELGV
                     HTLAPLVTERIIPRVSPVHAPKFQERWERIALEAAQQSERWNVPTILPIQTFQEFLQR
                     ENGGILIMLAERLEDISLSKILLPPEIQGGITLFIGPEGGWTKEERQAAQSLNVVFAT
                     LGKGILRAETASLASLAILQARLNYL"
     gene            complement(19913..21037)
                     /gene="dnaJ"
                     /locus_tag="PKM72_01875"
     CDS             complement(19913..21037)
                     /gene="dnaJ"
                     /locus_tag="PKM72_01875"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNP59554.1"
                     /translation="MAQTGKRDYYEILGVDRNASEEEIKKGFRKLARQHHPDLHADPN
                     HKKTAEEKFKEAGEAYEVLSDPDRRKKYDMFGHAAAGQGGGADGFGFGGGGFGDVFGD
                     IFEDFFGGGRGQNRAEQGNDLQYNLEISFEEAINGKEAKLKIPRWEVCETCHGTGAKS
                     DQAVKPCSACRGAGQIRLQQGFFTINRTCGQCQGSGQVIADPCKTCRGQRRVHKERLL
                     AVTIPAGVETGMRLRLSHEGEHGMNGGPPGDLYVALSVRPHPHFQRKGQEILYDLRLD
                     FVTATLGGKVEVPTLNGTTMVKIPGGTQHDQILRLKGLGAPGLKSQSRGDQLIRTKIE
                     IPTKLTPKQHELLTAYAEECGISTDTQGEGLFDKVKNMFD"
     gene            complement(21110..23041)
                     /gene="dnaK"
                     /locus_tag="PKM72_01880"
     CDS             complement(21110..23041)
                     /gene="dnaK"
                     /locus_tag="PKM72_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNP59555.1"
                     /translation="MSKIIGIDLGTTNSCVAVMSGGDPTVIANSEGARTTPSVVALTD
                     KDERLVGQIAKRQAITNPENTIYSVKRLMGRKHSSPEVKNAANRLSYKTAEGSNGDAH
                     VTIRGKSYSPAEVSAMILQKMKQTAEDYLGEKVTDAVITVPAYFDDSQRQATKDAGKI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDVSILEIGEGVFEVKSTNG
                     DTFLGGDDFDLRVMDWLVEEFKKDQGIDLRNDRMALQRLKEAAERAKIELSSSQETEI
                     NLPFITADASGPKHLVLKLTRSKYEQLVNDLIERTMEPCKKALADADMTTSDINEIVL
                     VGGMTRMPKVIERVKQFFGKEPHRGVNPDEVVAIGAAIQGGVLKGDVKDVLLLDVTPL
                     SLGIETLGGIFTKLIERNTTIPTKKSQIFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGLPMAPRGVPQVEVTFDIDANGIVHVSAKDMATQKEQSIKITASSGLSKEEVE
                     RLVKEAQSHTEEDKKKRELVEVKNQADTLIYSTEKNLSEHGDKIEESEKSNITGAVDA
                     LRKAMEETDLEAIKTAMQNLTTASHKLAEEMYKKASTDASSGGTGPEDGPGPSGGESP
                     GNEEKVVDAEFEEVDKGKN"
     gene            complement(23038..23643)
                     /gene="grpE"
                     /locus_tag="PKM72_01885"
     CDS             complement(23038..23643)
                     /gene="grpE"
                     /locus_tag="PKM72_01885"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNP59556.1"
                     /translation="MSSTDETTEEVTAEEVGPQEDPTEDTTGNTSECGETPEEELQIS
                     QDKYLRLAAEFENYKRRAQRDQSDSIRFGNESLLKNLLPIIDNLERAIQCAKDTGTSG
                     PLLEGVELTHKQFLETVGKVGVRQLCTTGNSFDPAIHQAVTQVESEGVDPNTVIEEFQ
                     KGYLLHDRILRPAMVSVAKEKSELTQPCSTEESGEEGGIQV"
     gene            complement(23813..24589)
                     /locus_tag="PKM72_01890"
     CDS             complement(23813..24589)
                     /locus_tag="PKM72_01890"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNP59557.1"
                     /translation="MLLAIDIGNSQIVCGIFQGSTLISHWRLSTDHSKTPDEYSIVLR
                     SLLQFHKILPEAISGCIVTSVVPPLTHIFDMLAQSLLGQQPLMVTSACPHGLILQYSN
                     PDEIGTDRLVNAAAAFARYRRYLIIVDFGTATTFCIVTQLGEYLGGTIAPGLKSAADT
                     LHTKTAKLPKVDLAIPASVIGKDTTSSMQAGIMYGYAGLVDEIVRRIQHEIGDSPLVI
                     ATGGLAQTIVPISRTIQEVRPNLTLEGLKLLYDRMTPSCG"
     gene            complement(24631..25455)
                     /locus_tag="PKM72_01895"
     CDS             complement(24631..25455)
                     /locus_tag="PKM72_01895"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNP59558.1"
                     /translation="MLSAQKLAQLLPLKKIGEALYIFDELASTNAFAIERARDQALSG
                     TVVLADRQTSGRGRLDRLWFSPGKSNIYGSLLLVPETTIQYLGWIPHMAGVAIAQALE
                     QQTGIRIDLKWPNDLLIGGRKLGGVLCDSFRNAGHQTCVVIGFGINVNLSQSEFPSEL
                     QTSATSLHIHCHCPLDREALITKVITSLEENWENLRSNGPQSLLEEYNHWCITIGHTI
                     QVQFPDGSQLQGLAHSIGEHGQLRVIPSPSDPNEQSSRMRDIHSGEILHLRTTAQQ"
     gene            complement(25483..26397)
                     /gene="nadC"
                     /locus_tag="PKM72_01900"
     CDS             complement(25483..26397)
                     /gene="nadC"
                     /locus_tag="PKM72_01900"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNP59559.1"
                     /translation="MSAPSLASPPPLSSMRRVISAALEEDLAYGDLTSTLLIPPTLLA
                     QADIVAKSHMVVAGVAVAKEVFQAVDSTIAFTTHFDDGTRVPPSTPVLTLKGKAQSLL
                     QGERIALNFLQRLSGISTLTFQFCEAVRDYPVAIVDTRKTTPGLRALEKWAVRLGGGK
                     NHRFSLYDGMLIKDNHLMVMASQGMNITQTCLWARQHAPHGLRICVEVETIAQVRQAL
                     KGKADVLLLDNMTPDHVRQAIDIIQKQALVEVSGGMTLDNIREMAEAGPNFISIGALT
                     HSAPSMDLSLDMTPLRTKTVPQKRKYRT"
     gene            complement(26397..29183)
                     /locus_tag="PKM72_01905"
     CDS             complement(26397..29183)
                     /locus_tag="PKM72_01905"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNP59560.1"
                     /translation="MAFSQLEKTYNPGEVENRWGSYWIEHQLFQPDPTNPALSFSLVI
                     PPPNVTGSLHIGHALNTSIQDIIVRWRRMQGLNTLWIPGTDHAGIATQNVVERQLAQE
                     GQTREHLGRPAFIDRVWKWRHQSGDTIIEQLKQLGASCDWTRLRFTMDEGLSLAVREV
                     FVRLYEEGLIYRGERLINWCPRCLTALSDIEVEHEPTKGKLYHIRYSLADDPTQFLIV
                     ATTRPETILGDTAVAVHPEDPRYQKYIGTKIRVPLTTRTIPIVADPILVDREFGTGAV
                     KITPAHDFNDFEAGERHGLSRLVILDYQARMSPMGLQEAQANPPLQAELHSKSVQEAR
                     GIVIEALEKQEFLSRVEDHAMAIGKCYRCKTVVEPYLSPQWFVKIQPLATPAIQAVEA
                     GDIRIIPDEWKNNYLGWMRQIKDWCISRQIWWGHQIPAWYCRNCNTDHLPKTSQAGEA
                     PDQEVIPPAQPTRSCFIASDAQPIVSRTPPQSCPTCGGTNLFQDPDVLDTWFSSALWP
                     FSTLGWPENTEDLKTFYPTATLVTGLDILFFWVARMIMMGLKFTGKVPFRDVYIHALV
                     RDAEGQKMSKSKGNVIDPLTKMRQYGTDALRFTLASMASPGRDIKLAEERIEGYRNFV
                     TKLWNAARFIHLYAEGPRVSLSTGLRPFPDRWILSRLNHTIHEVTKAFEDYRFDQAAS
                     ALYQFIWHEYCDWFIELAKPCLQQENHPDAPATRQTLLETFKIIQRLLHPVMPFITEE
                     IWQSLPHEGISIMTQPFPTEKPEWANAEAEQAFLFLQAFVNTARTGQNFLNISSTQTP
                     LIYGASTQANHTATLTFLAPYIESILRSSVITDQGIPSLRTLQLPSGYFSTIGIPVPR
                     EIDLHEVVKKIQKQIGEKEKEVQRLGNRLSSSEFREKAEPSVIQDSEDRRTKLVDELG
                     ILNMTEQQLASMKT"
     gene            29616..30338
                     /locus_tag="PKM72_01910"
     CDS             29616..30338
                     /locus_tag="PKM72_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNP59561.1"
                     /translation="MSSSPIKVIFFDAVGTLFDVKGSVGEVYLTYAKKYGVPDTEQTQ
                     QALNVAFKQTMREMPLPVFSVERPEKLKQCERLWWFDVVHAVFYRVGMFEGFDDFFEE
                     VFEAFGQSTHWQLFPDTYEVLGHLKRQGFELGIISNFDSRFFQVSRGLGLDTFFDSVT
                     ISSLVGVAKPAQRIFCHALDEHMLVPQEALHVGDHPIEDFDGARQAGLHAVLIDRSSN
                     AASHPHTLSSLTQLSSYDLLNS"
     gene            31195..31500
                     /locus_tag="PKM72_01915"
     CDS             31195..31500
                     /locus_tag="PKM72_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59562.1"
                     /translation="MKGGKSFPARVAPGAKLRRRGGRIPLIAHRARRLDQHPGSPLPY
                     AGKRQSGQTSAGAAIGPESASSADAHSRQTGLRSPRARDGRHAHPNEWGASSTKDRT"
     gene            32050..34269
                     /locus_tag="PKM72_01920"
     CDS             32050..34269
                     /locus_tag="PKM72_01920"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNP59563.1"
                     /translation="MEFFAISGLLNGLAASGLASFVYFRAPYDPRHWTFGLFGIATAL
                     WSFGYFAWQVSDSEFYALLNLRILMAGAIFIPITFLHHVLYLLGKEIAFSGTIKWNYL
                     LGGVFLLTDFTPFFIEGVRQISVFPFWGVPGLIFHFCLIWWVGLVIFAHFLLTQAYVR
                     EKGLRRRQFLYLLIGSGIGYIGGATNYPLWYGIEILPYGTIGFAVYISIVAYTLLRFH
                     WLEFSVYVEKGLSYFAILLFVSQPVYPMLLLAQKSLLGAINVRFSVVQLVLHLMTVVG
                     VYQMKVGTKGAVARTILKGRELRTQALSRFSSKVANIQNIDDLGQAILETVGRSAGAS
                     KAALFVLQVEENRYRAVSSFGFSHDHPVIQNGWAISDNLPQLLLFTQSRVSIKELKGI
                     DSNEGEMRIAQILEEAGLEVFYPIFGNNQLLGVLALGPTSSEAIRMMGGKTFWNMIIQ
                     ESALALENAILREEIHRSQNLLCQVDRLRSLEAMANGLTQELHNPLVSIKAFVQVAEL
                     RRHDGEFMDRLHRIVGEDLAKIDALTKEIREYVKPLSGSLNAKTHVHDIIDSCLMFVA
                     SNPSYQKTMIEKIFSSDVPMVSIDRQGLMQAIFNGLLFLLKDPSKLSGTLRIETEADR
                     FVRGQNWLQVSVWWKAEKAVMDSELVSMEEWGFDDSFIDEPDPSATQGLILAHQIIQR
                     HSGRFQVWTNKRGILGFQIQLPLSLPSNNVYPLTSIRNPAIPLKPVKVSPESGPLFS"
     gene            34347..36635
                     /locus_tag="PKM72_01925"
     CDS             34347..36635
                     /locus_tag="PKM72_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNP59564.1"
                     /translation="MNSFLQNEHAAFDQVPLVVYRCIQQGIDRRFEFVTPYVYSLLNL
                     TSEQLIQDSKAFFCRIHPEDRNMFLRGWENHTNLPATIRLEYRMLGEGSRVIWVQENS
                     VISKGSIENEIVCHGALIEMPHHQQIKEELQRWDRELQSLTDNLPDIVGRFDRKNRVM
                     YLNRWWDSVEPFPPEKYLGKQLIELGVSQKVADIFEEKIQSVCANGRSDSLEISHPTK
                     QGLRNFEIRFCPEPKINGHISTVLLICRDVTDVRMAELAFRESDEKFRQLAEAVDSVF
                     WIWDVDLQQIVYVSPAYKRHWGGDPQKLMSNPFDWLTIVFPEDQSKVENLFLKRIDVK
                     CLDIEYRIVTRHNELRWVHNRTFPVKDSLGRIHRVIGIAQDVTERKKWEEERLRGAKL
                     ESLGLLAGGLAHDFNNLLTAILGQLSLAKFSLDSTNPLFNRISEAEHASLRAQDISRQ
                     LLTFSKGGAPVKKTVLLQEIIKENVQLVLSGSNVRPIFNIADDLWPVTIDAGQICQVI
                     HNLVINARQAMEKGGECIIQAHNVKGDMVERSGFGPMALHDGEWIEIRFMDKGVGISK
                     ENLEKIFDPYFTTKSTGSGLGLATSYSIVRNHGGVLAVNSTLGQGSTFSLFLPAIPIP
                     KIAYEVPERRVKMGQGKILIMDDEIQIRKVLGEMVETCGYSYQTAKDGEEALRDFCEA
                     WEVGSPFSAVILDLTVPGGLGGKEVLSRLLTIDPQVKAIVVSGYSNDPVLANYQEYGF
                     KGRVAKPFNLVDLSVVLHSVLE"
     gene            complement(36803..37756)
                     /locus_tag="PKM72_01930"
     CDS             complement(36803..37756)
                     /locus_tag="PKM72_01930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP59565.1"
                     /translation="MFPHTISLSQFFPRSATEIQKLSQSTIELISGFSKKGGPDPTDY
                     PILNSLLGQFSGENIHKVFSADQIRSIRFAFGEALSPETIQGWAYHKPLGYAGDYQII
                     EKIYNYYTSSNPSLTKWDQFFHSQKAPKAVRNRLSFFLDQLWRTKMETPSTSHILNLA
                     SGPGRDMYEALKVLGTSKLEIDCVDQDANAINYAKDLCRDFLAHIHFTHKNVFRFSPN
                     KTYHLIWSAGLFDYFNDNIFKRTLKRFFTLLKPNGKMVIGNFTTDNPSRGYMELFKWD
                     LFHRSKTHLRRLAQECGFTENQIRIEQEPEGVNLFLVIMKN"
     gene            38330..39037
                     /locus_tag="PKM72_01935"
     CDS             38330..39037
                     /locus_tag="PKM72_01935"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNP59566.1"
                     /translation="MKKITGKKKSNPSVRVVYVIPKTKKAQFQKRLLAWYGEFGRDLP
                     WRRTSDPYKILVSEVMLQQTQVDRVIPKFHEFLGKYPTLQDLAEAQPEDVRKTWYPLG
                     YNIRPYRLQGIACETIERYGGAIPHKPEELLSLKGIGRYTAGAIRSFAFNKDAPILDT
                     NVMRVLHRIFIGKGEAKKQKAELWLLSEKLIPRGKGYDFNQALMDFGAMVCTARKPIC
                     LLCPMRNICLTVSLDEK"
     gene            39051..39437
                     /gene="mutT"
                     /locus_tag="PKM72_01940"
     CDS             39051..39437
                     /gene="mutT"
                     /locus_tag="PKM72_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNP59567.1"
                     /translation="MPPIVVAAAIIWQKDRVLLSRRKPESYLGGLWEFPGGKKEFGET
                     LEDCLRREVKEELGVEISEPVWFHSLQYQYPEKMVELYFYTCSIILGIPQALESAEIT
                     WVRKHELISYEFPPADIPVINKIMQI"
BASE COUNT         9717 a         8471 c        10260 g        11221 t
ORIGIN      
        1 gtcctcttat tctctcgaag ctcacgattg aaaaatatat ggtattggga actggacaac
       61 tccaacagcc acacccggta cctcaggcct accaggatgc gatgatgtta tctgccaagt
      121 tctagtcggc tttttgatga aatatgcatg gccggtaccg gtgtgttggc tatgcatatt
      181 cccatgcctt cgttagtcaa atcccgttgt ttgccttgat cgtttttctc cgttcctccg
      241 gtctgccatc gtttccggta ttcaattttc ttttgagtga atttcttact cgggttcatt
      301 atgggatatg aaatggaaga aaatactgaa tatcatgcgg agcttttccc ttatttgcag
      361 tcttgggggt tgcgagaatt tcgtgatgag gcgtcatact atggctggca acgggagacg
      421 ctttctaaag aggaattaca cgattttcaa cgattgatcg aacatcgata cggtgaagaa
      481 aacggaaagg gcgacattga gttttatgat ggattggcac gtcgggatct tattcccatt
      541 ttgtattccc agcggttgca atattttttg acgataggca ccgctgtgtg tcgccggatc
      601 gcaccggctc aacaggtttt ggactatggt tgtggggttg gcattctaac tatatttttt
      661 gccctacagc atcctgacgt tgaatttgtg ggaatcgatc gatcgcctcg atcggttgaa
      721 atggcttgtc ttgaggcaga aaaacgacaa atcagaaatg ttcgctttga gatctctcat
      781 gtgccaccac aacagatttc cggaacttac gatctcattc tttcgacgca tacgttgttt
      841 caagcggaac gggagccggg gcttccaagc cgaacctggg aaacgtttca acgtgccaat
      901 gaccctcaac gacaggagca gctggaagtt ctgacgggag tacagggacg tttggatgcc
      961 ttgatgttgg tattagggtc gattgggaga atgctccttt ttgaaaaaac ctggaatctt
     1021 ggtcggcgta ttttctttca acgtgcgtta ggtgccagag gcttgtttcc catatcttcg
     1081 ccggttttct gccggtatcg gtcagtggat gatgaggtga ccgatggacc cctgtatgag
     1141 gttgcccgga tcacacaggg tgtggagact catggatgga atgaggaacc atatcgggca
     1201 tcaggtgaaa cactctatcg gtgcatcggg attgctgccg aaaggatggg gcgggtgttg
     1261 gctacagatc aagtgagtgc cagtgtttcg ggtgttcact ccagtatggg gtcctggatg
     1321 tttcgcttcg gactgtggaa ggaaatcttt gcttggggtt tgtgtcagta ttcctccggt
     1381 ttgacaggcc tggttattgg agctggggcg gatcgggatc tattacatca attggttgaa
     1441 actgttacct acataaccga gtctgacttt caacagttgg ttcatgactt ttggggcggc
     1501 atgattcacg ctggggagga tcaatccgcg ccttgttacg aaaatcacca ttcctctgca
     1561 caaatcatct acgaaggact tccctccaaa cacgttcaac gggagtcgac ttttcaagat
     1621 ggtgagagag gggaaatgca tatcgagttg gggaccacaa cccatctgac atatctttat
     1681 ttcgccaatg ttttcgatca acgtcagctt gtacttatgg atgaaggtcg agctgagatt
     1741 ctaagtgaac attatcgaga gtccctccag catcgacacg gtccaccttc ctgagttcct
     1801 tcaatccgag tcttcggttg gttttttagg gagtttcaat aacctgcaat gtgcgctgtt
     1861 gcgttttggc atagcaggcg actgcttcat atgttttctg ctgaatgtct gagggaaggg
     1921 ttgcccaatc ggaagtgagg ccaaccgtaa gtatgcggcc attatctctt attcccttaa
     1981 ttcttcctat ctgtaaataa tcaaggagga cgtctccatt gacttcccct gaacaggctt
     2041 gggctgtgaa agtggtgttt gtgttccgat tcccatactc gattgagatg gttaccgacc
     2101 agaaaagaag gcttaagaag aaaattatgg ctaatcgcat ctcactatat cccaatactg
     2161 tgaggagtct gtctgaccga ctcctaccgg taaatacctc agagggagga ggcgaggaat
     2221 cacgaaatga cagcgcacca gggtctgtag aaggagatgg gattggagga gatcactaga
     2281 gcagaaagcc taaatcaagg ttattcaaga atggttttct taagatggtc tgggaaattt
     2341 ccgacaagaa gagcgaggct gtgaaatgca ttcgctaatg gttttattga ctgccttaat
     2401 ctgttctata gccgagaata ttgggggtga ccaaacacga tagagggagg ggcaacccgc
     2461 tttaggtgaa gaaggaaggt ctatgtatta cgggggaagg atgtcttagc tagatgaatg
     2521 gtgagacact tttgttttgg gtgttgctcg caacctgtct gacgcattgg ttgacggttg
     2581 ttttgcttct tcgtcgtgta cgttcgactg aaaataccct ggtatggaat ctcttaagtg
     2641 tggcagttgg gctcctggct attcagaaga cttatgggat aaccattccc taccttgaac
     2701 tgaatcctcc tccagtgaat tttgtgagta catggctggg ttttgttgtc gccggattat
     2761 tgttagctgg aatcatgcgg ttgggtccct ttctgtatct gcttgaacgc aataaggagc
     2821 tattagccgt cattgaggaa cgaaatgtca ttattctgca cttttatgaa cgtattgccc
     2881 gcgccttgca aaaaattcaa atggctatgg aggtagggcg tcccaccaac tgcattattg
     2941 atcaagtggc cgaaatgtca gagatgctca aggggttttt ggaaaatctc aaggccggag
     3001 ttctgttggg gaataaattc gaggtggcac tcaagacact ggtagaagat ttgagtcagg
     3061 aagcttcatt cccgatactg gtcaatgtcg atccctcttg tgaggatcaa ctgtcacgcg
     3121 agcagggtgc tcagctgcta catattgtac gagaggccgt tctaaatagc atacaatatt
     3181 cgcacgcgaa gaagggacag gtgtctgcga agatgacggc tacagacacg gttgtggagg
     3241 tgtcagataa tgggaagggt tttgaagttg atcttgtccg ggctcaagga catggattag
     3301 gaaatatggt caatcgggca aagaagatag gggctcgttt gaaaatccac agtcaaccgt
     3361 ctaaaggaac ttcggtattc attgaagttc cattaaatga agcgccggct gagaatcgtc
     3421 attcggcatc aaccgtagat tcctcatcga atgcgaccca acgcgctccg gtcggataac
     3481 ttttgtgtcc taatgtgtcc caacggggaa gcgtcggctg tttcctggga tcaagggaga
     3541 ggaagggaaa taagaaactt ggtggcggtg gagggatttg aacccccggc ccgcggctta
     3601 tgagtccgct gctctaccac ctgagctaca ccgccgtatt tcaccaagaa cgaaaagatc
     3661 tataccatag ctgaagaact ttggacaatt ctttttctgg aaatgatcaa aatttttggc
     3721 tgaatttttt agtggtggtg tgcaggattc agtgtagcac ctcctttact tattcgtttg
     3781 aggacctggt aatcgtgggg atatctctaa agttggtgat ttttaacctt gaaaggtttt
     3841 tatgacttta acggctgctc caaacatcaa gcactccgtt ctggttgggc gacaggccat
     3901 ttttgatcgt gaaaaaggtg tttttggcta tgagctctta tatcgagatg gtgaagccaa
     3961 tagtgcacaa gtgattgacg gggatgaggc taccgccagg gtcatggtga atacgttttt
     4021 agagatggga attgaccaga ttgctggaac gggtcaggcg ttcatcaatc tgacagcgaa
     4081 tttcttttta agtcaaaatt atgaagtcct gccaaagaat aatgtggttc tcgaggtatt
     4141 ggagtccatt gagccaacgc ccctcgtcct ccaatctctt gtcagggcta ggcaacttgg
     4201 gtataaaatc gcgctggatg attttatcgt gcgggattct caccgagcat tattagaagt
     4261 tactgacttt gtgaaagtgg atattttggc cttaacgagt gaacagcttc tggagcaaat
     4321 tgatgtgttg aagcaatatc ccgttcgatt gcttgcagaa aaagttgaaa atcaagaggt
     4381 gtatacgttc tgttatgaac agggatttga atattttcaa ggatactttt tttgtaaacc
     4441 gcagatcatg gagggagtca agctttccgg aaatcgtatg gcgatcgtgc tgttattggc
     4501 caaattgcaa gatcccgata ttaaaatgga agatcttgag gcgttggttg aaaatgatct
     4561 gtcgctgagt ctcaagctcc ttcgttttgt gaattctgca tctgtgggcc ttcctcgggt
     4621 ggtcaattca attggacaag ccgttgggat ggtagggacc gaacgtatgc ggcaatgggc
     4681 ctccttgctt gtgctggcta ataccggcgg aaagccaagc gaactgatgc gcattgcgct
     4741 gatccgggga cgaatgtgtc aagggttgag ccggctacaa ggggagtccc caagccaagg
     4801 ctttaccatt ggcctatttt ctgtattgga tgcctacttt gattgtgaaa tgaaacaact
     4861 tcttgcaggt cttcctcttg cgtctgaagt tctgcttgct ctgaccgaac ggcagggatg
     4921 ctttggagaa attttaacct gcgtgatgtc ttacgaacga ggagattggg atcagattga
     4981 ggatagtcga tttgagcccc acgtaatacg ccatgagtat tttctcagta ccgaatgggc
     5041 caatgatgtt atgaaagcga ctttaggggc gggggacggt acctagcggg aaaggatacg
     5101 gggaaaggct tgaaggccag aattatcccc ttcgtttatc gtcctgttct cctgcaaaat
     5161 aattcaagac accgacaccc ttttcactaa gctcaatagg tttatcatcg gattcggggg
     5221 ccagttgatc atcagggagt tgggggtaaa ttttaacacg taagctttct tccaatcctt
     5281 catctgaaaa atccatatca ttgagcagag cgtttaatcg ttggaaagct aaaatggaac
     5341 gggcttggga aagccatgtt ccggaaaatt ccgtatcatt tactttggta atacttgtcc
     5401 aaaacttgga ttcttcaggc tctgacatac ttccctcgac aaatgctcgc aattgatcaa
     5461 gcaacgcttc ctctgttttt cccaagccat cgtattcaac cattgacttt tcaaagggac
     5521 gctgggacaa gaagtcaagg gcttctttcc ataggtttat gggttgcaac ggctcatccg
     5581 gcgctgagtg tttgtcctga tgcttttgta attcagtcag aaactgttga agggctttga
     5641 cctttctggc aacgagactc cctaaaggaa ttccccatat atgggcaatt tcgtgatcct
     5701 gcaatgggga atttcctgaa acgatgaaat cattttgagc aaatttcagg cgacgcctga
     5761 atatcccacg tcgtcgcaat aaagactggt attccaggac caatcgttgt gcctgatact
     5821 cctttgtgga cagttcctga ttatcccatc cacgatctaa ttttcggagt tcctggcgaa
     5881 gtaggatcga gggtgcttgg gttttagcat cctgcaagcc attctgagtc aatccaaatt
     5941 ctgatgtgag taaccgctct gcaatctgaa tggtttcatc aaggattttt aattcttgag
     6001 tcaggaattc tatgtgcatt cctgggcgct cacgtttttg cctgtagtaa tccctgtact
     6061 ggttcgcacg tgatgtgata tttcgttcgg cttccaggga gagcgggttc ccttttggct
     6121 ggttgcctgc ctgaaagcgg gaatcgggtt gatcgttgac catataggcg atggattcag
     6181 gactcaggtc caggtactgc aacaaaagaa gttgcagcat ggtgcggcct tgagttggaa
     6241 gtgtctgaat ggctgactcg atatgttcaa aacgtagagt tgtaggcata agcattaatg
     6301 aactcaagtg caagagttcg gatttttcac cttaaaagtt ttatggagtg ccattatcaa
     6361 gagattcgag acatgtggct gcgtattccc ttagttgctg taccgttccg ttggcataga
     6421 gttctctggg gatttcaatt ttgaccaatt gcgatggatc aatcccccgt tctgccgccc
     6481 aatcttcgtc aataaataag gtcactgcgc catctttgtc tcggcgcacg aaaagaacat
     6541 ctttttcccc ttccattctg actctccaaa aatgaaaatt aggctttggt tttcttgata
     6601 acacaccgct ttgagacctg tcaaaatggg gtcgtgggcc taaccaataa tttctaaaat
     6661 ccaagaaact tttaaattgt tggatgtcgc aggtaagagg ataagatagg tgaaaaaacc
     6721 aggttgcgtg tagtagaaat tccctagcaa acggtgtttg tcatgaacca tcaaatgacc
     6781 gaatgctccg tcctcctgtt tttctgtcta gcagtgataa tttacgtcct cagattaggg
     6841 gacgtatatt gggaagcatt tacaggtacc cttcggtaag ggtacctgtt tgaaagagtt
     6901 atcctggggt agtgccgagt ggctgttcga acatgcggtt cgagattgat tgagagaggt
     6961 tacccgccca cggtttcgac aacttcttta agacttttca ttatacaagt gaaaattggg
     7021 atgtcacgtt tgacgtaacg gctgccttcg gtagagcgta attccatgac gaggtccaga
     7081 aagtcctggg gagagtcggt ttcaaaggcc accacaaatt cctggtcatc caaaccaaac
     7141 gaataggtgg tattgagttt gaccgagggg tacttgtgcc caatagcaat atgttcatcc
     7201 atcatgcctt ggcgggcagc tttggtgagc agataccact ctctggtttt ttcaaatgga
     7261 tacacaaaga tatatttcgc acggccggga gaaattttca atcttcgact ttcctggccc
     7321 tcatgcacat gatggtctac gtatatggat cgtttcgtca tagaaagata ggaatacggg
     7381 gtagtaaggt attgccccaa gcctgtagcc aagagttttg aactcatttc ttgaaacatt
     7441 tccaggtcat agctaattcg ccacaacaga aagtcgcaat cgcctcgaat gcctgttgtg
     7501 gtgtaaggca cgacaatgac tttaccagta tattcttccg ctgcgcggac aaattcttgt
     7561 ttgccctggt cccgcacctc tttgggaaga cgtctccata aagggtcgac tttatagaaa
     7621 gcaaaattga caaattgttg tttggcagcc tcttcggcac cggccatatt catacctccc
     7681 ctgaaatgaa aacacgtgtt tttttaatca cttaaaaaaa taggtcttaa catcgctacc
     7741 gcaatattgt caatgcgcac gttgcgtgat gcctgttaag atccttttgg acacggagga
     7801 gtgcggagta gggccttagt ccggagggag caacatttgt agttcatggt tattgatgcc
     7861 aatcttgtta tttcaaccat tcctcaaacc tgtggggact atgggtagat cgataagatg
     7921 tgccggtttt ttgatgcttt ttgaagtggg attggtcatt ttagcttcct ctccggtgtg
     7981 gggcattgtt tcagatccct cgcaatccca aattcttgaa gccgttcata ttggtcaaga
     8041 gggagcaagt agtcgaactc cacccaatat gttgtatcgg catttcggtg ggtcttcttc
     8101 tgatgttcct caggcccagg gatttcttat gacaaaatta aatggaattg cggtgttgtc
     8161 ctgtcatttt gctcttcgag gagaaaatcc cggctcagag gatattcaac gagtcctgga
     8221 cgaaaaagcc ttgcaagtcg tggtggcaat tttcggggat tcacccacgt ttgccaataa
     8281 cagctatctt ttgctcaaac aagaaaatcg gttgatcaag cctgatcata ttcggtttga
     8341 tgccagagct tcccagatca gccaaagcca agaacttccc atatttcgtg caaagattgt
     8401 ggcgtccttt aactatgatg cttttgatgc cctggctcag accactatca aggttttccc
     8461 tggagcggga ggcgaagtga cctttgatct agattttgct gacattcctt gagggtcatt
     8521 ggatgaataa acaaccgatt gatgccatct gacggaatcc cttgaacttg aagctttttc
     8581 atgccgaaat cattgccgtg ggctcggaat tgcttctggg cgggcgaatc gatagtaatt
     8641 cggtatttgt tggacatctt ctcgcggaat gtgggattga ggttcggaaa aagatctctg
     8701 ttggtgatca gaaaggtgag attcaagaag cccttcgtgg ggcattgaag cgcgctcatg
     8761 taattgtctt gaccggtgga ctcggttcaa cactcgatga ttgtacccga gaggctgtag
     8821 ccgaaacctt tcgatgtccg ttgattaaac gcaaaagagc gtatgacaat ctcaaagcct
     8881 gctatcgacg atttggaaga ccggtcacgc caatattggc caaacaggcg tttcttcctt
     8941 ccggagccac gatgttggcc aataccgtgg gcacggcatc aggtttttta atgcgccagg
     9001 ggcaaacggt cattgtggtg ttaccaggtg ttcctcgcga agccaaagtt atgatgattg
     9061 atcaggttca accgatactc aggaagattt ttaagagcaa aagacactat tggttgcata
     9121 cctttcatac ctttggatta ccggaaaccg atgtccaaaa ggcattgagt gctgttttga
     9181 gggaagatgg acctatccaa tttggcctcc tggcctcgcc caggggagtg acggtgacgg
     9241 tgagttgttg gatggtcagc gactcccaat cttttgcgaa ggaaaggtcc cccttctttc
     9301 cggaatgggc tgacctagtc cgtcaggtac gtgaatgtat aggaccatgg ctctttgcag
     9361 aaggtgaacg caccatggag gaaatcgtcg gagaagctct gcgaggtctt tcctggacgg
     9421 ttgcggttgc cgaatcctgt accggagggt tagtggctca tcgcctaacc ggcgtacctg
     9481 gaagttcgct gtatgtggat aggggagtcg tgtcctacag taatgaagcg aaacaagaac
     9541 ttgtgggggt tcctgcatcg acccttcggc ggtatggggc ggtgagctcg caggtagcct
     9601 tggctatggc caaaggaata cgaaggcgaa gccgagtcaa tgtaggggtg ggggtcacag
     9661 gaattgccgg tcctggtgga ggtagcctag acaaaccagt gggtctggta tatggcgcga
     9721 ttgatggtcc gcaaggtccg cggtggtatc gttggcagtt tcatggggac cgcgcggaaa
     9781 ttacactgaa gacatctcag gctgttttgg atttaattcg tcgatatgct aatgaagctt
     9841 cattctaacc ggaatagtct gagagttttt cttgcggtgg aactctcctt ggacctgtgc
     9901 cggaaagtga gggagtttca acaacagctt cagaacttct tgccacatgt caattgggtt
     9961 cgccctgaat cgattcatct caccctgaaa tttcttggta atgttgacgc tagcatggtg
    10021 gaccaggttt tgaccgctct tgagcccatc ggaaaaagtc agccgccttt cacattagag
    10081 attcagggcc tgggggtttt tccacacgtt cgacgtccac ggatattctg gattggatgc
    10141 acgggagaca ttcctgcctt gcttaatctt gtttccggag tcgagggcgc gttggacccg
    10201 ttagaatttc cctccgagga gaagccctat gttcctcatt tgaccctggc caggattaaa
    10261 catgacaatt ctcaggtggg taaggtactg actcattcgg gcttgttgga acaacctcag
    10321 aatcttggga tattacctgt tgcccggatc actctttttc gcagtaatgt gaccacgatc
    10381 ggtgtggaat atattcctct gtgggcggtg ccattaataa atgaggcagt atcaaatttc
    10441 tcaagttaag atagccattt acctgttaga gagggtgggg taagatagcc gccactttgc
    10501 acgaccaggg atattgaaca aaaggacacc taatggcaga acgcgtaact cctcaaaaag
    10561 aaacacaaaa agacggaaaa aaacgagcat tggatttagc tctgacccaa attgaaaagc
    10621 aatttggcaa aggggcgatc atgaagctag ggacggacaa cgttcctcat gatattcctg
    10681 ttatttctac agggtctctt gggcttgata tggcgctggg aatcggtggg ttgccacgag
    10741 gtcgcgtcat tgaggtgttc gggccggaat cgtccgggaa aaccacgctg tgcttgcatg
    10801 cgattgctga agctcagaag gccggagggg cggccgcctt tgttgatgct gagcatgcgt
    10861 tggatatttc ctatgccaga aagctgggtg tgcagacgga tgatctgctg gtggcccagc
    10921 cggatacggg tgagcaggct ttggaaatca ccgaaacgtt ggttcgaagc ggagcgttag
    10981 atatcattgt cgtggattca gttgcagccc tggttcctcg tgccgaaatt gaaggggaga
    11041 tgggcgattc ccatatgggg cttcaggcga gattgatgtc acaggccttg agaaaattaa
    11101 ccggggccat ttcaaaatct caagcgtcgg tagtgtttat caatcagatt cgtatgaaaa
    11161 ttggggtgat gtttggcagc cctgagacca cgaccggcgg caacgcgttg aagttttatt
    11221 cttcagtgcg gttggatatt cgccggattg aatcgattaa agatggtcag gatgtgatgg
    11281 gcagtcgggt acgagtgaaa gtggttaaaa ataaaatggc cccacccttc aagcaagccg
    11341 aatttgatgt catgtttgcg gaaggtatct ctaaagttgg ggaattagtg gatttaggcg
    11401 ttgagcgtcg aatcgtggat aaagccggag cctggtattc gtataaggag gaacggttgg
    11461 ggcagggccg ggaggcggtc aaaacatttt tgaaaagtaa tccggatatt gccaaggata
    11521 tcgacacgca attacgaaac agctatggcc tctccgtgga ggctcaacaa aaagaggaat
    11581 catctgcccc tgccagttct agtggaaaaa aatcaacagg ggatcgtgac tgatcttgcc
    11641 gagtggcaca ttcatcttga ccgaaaccca atgatatgat tgcaacatca cttgaacttc
    11701 gacaggcgtt tgtcagctat ttttcgaccc gtgggcacac cgtggttccc agcgggccgc
    11761 tgattccaca agcagatcct actctcttgt ttactaatgc gggcatgaat caatttaaag
    11821 gtgtgttctt gggcgaggaa tctcgaccgt atagccgggc cgtctccatt caaaaatgta
    11881 tgcgggccgg gggtaagcac aatgatttgg aaaatgtggg atttacccgc cgccatcaca
    11941 ccttttttga aatgctgggc aatttttcct ttggagatta ttttaaagaa gaggcgattg
    12001 cgtttgggtg ggagtttctc actcagattg ccggattgcc ggcggaccga atgtgggtga
    12061 cggtgtttcg tgatgatcag gaagcctatg acctttggca cacacgaatg ggcatctcag
    12121 agagtcgtct tgttcgtctg ggtgaaaagg ataatttttg gcaaatgggg gagacgggtc
    12181 cctgtggtcc ttgtagcgaa atcctcattg atcagggaga ggcattcggt tgtggtcgac
    12241 cgacatgcgc cgttgggtgt gactgcgacc gatatctcga aatttggaat ttggtgttta
    12301 tgcaatttga tcgggatctg gctggtactc ttcatcctct cccgaagccc agcattgata
    12361 cggggatggg gttggagcgg ttagctgcgg tgactcaagg agtatcctca aattatgata
    12421 gcgacgtgtt tcttcctatt ttggaaagaa tagggaaagc cactagccag gagtatggtg
    12481 cttccttggc ggctgatcgg tcgatgcgag tcattgcgga ccatctgcgg gcgatgacat
    12541 ttttaatcac tgatggcgtg ttgccgtcga atgaggggcg tggctatgtt ttgcgacgga
    12601 tcatgcgtcg agcctcccgg catggtcgat tattgggtat ggagcgggaa tttttatatg
    12661 accttgtgcc ctccgtggtt gatcgcatgg attcggtcta tccggacctt cgttcaatga
    12721 aagatacggt ttgcgagatt gtccagggtg aggaagcgcg ctttattgga acgttagaac
    12781 aggcattgcc tcttcttaat caaatcctga cggaggcgaa gcaaaagggg agccaaatct
    12841 tagatgggga agcggtcttt aagttgtatg atacgtacgg gttcccgctt gatttggtgg
    12901 aagatgctgc ccgggaagaa gggctagcca tcgaccatga cggttatcaa caggcactag
    12961 aggcgcaacg tgatcgagcc aggaaaactg cgaggtttag ccagggggac tctcgaagcg
    13021 cattggtgag tgctcttgaa cagtttcctt taacgcaatt tgtgggttat gcccaacagg
    13081 aaggcagggg gaggctcctt gctctggtgc aggatgagca tgtcatcaag gaggctaagc
    13141 aaggtgagac ggtggagtgt atcttggatg ctactccatt ttatcctgaa gggggtggac
    13201 aggtcgggga tcaaggcgcg ttggtgggtc cgtcggcaag aatcgccgtt gaggatacca
    13261 ctaagctggc gaaggggtgg tttctccata agggccgtgt catcgaagga tctgtccatg
    13321 ttggcgatgt tctcatcgcg acggtacagg gtcaattgag gcagaatgcg gcgagaaacc
    13381 atacggcgac tcatcttctt catgctgcct tgcgggaaat tttaggtccc catgtgaagc
    13441 aacatgggtc tttggtagcc ccaaatcgat tgcggtttga tttttcgcat tttaaaggat
    13501 tgactcccag aaatattgaa gaaatcgaag gactggtgaa cgaacaaatc cgtcaaaacc
    13561 tgcctgttat ggttgaagaa atgggaattc aggatgctct gagtcgtgga gcgctggctt
    13621 tctttggtga taagtatggt gagcgggttc gggtcgtaga aatggggtct tttagtcagg
    13681 aattatgcgg gggaacgcat tgcgttcgaa ccggagatgt cggcctgttt cgcatcctgt
    13741 ccgaaggagg aattgcggca ggcgtccggc gcatagaggc actcacaggg gaaggagcgg
    13801 tgcttcagac tcagcatcag gaagccgaat ggagagaatt gggcgctgtg ttgaaagccg
    13861 gtccaaatga ggtcgttgaa aaagccaaga aactcgttgg aactcttcgt gaaactgagc
    13921 gggaactaga gcgcacgaaa caaaaattgc ttgatcagca aggtgctggt caggaaacct
    13981 taattcggaa tatcggtggg atgccgatcc ttatccagcg gattgatggc ctgactattc
    14041 aggaattgcg aatcttttcc gataagctcc gccacaaagt gccttctggt cttttggttc
    14101 tgggatcggt taaagaggga aaggtttcgt tgttggtcat tgtgggcaaa gaccagactc
    14161 acaaaattcc tgcggggaaa gtggctcaac atgtggctca actcgttgga ggttctggag
    14221 gcggccgacc ggatatggct caggcgggtg gaaatcaacc cgaacagtta gatgcggcgt
    14281 tgcagagcgt ctatgactat gttgcctcac agataggtgc cttgacggag gagtaaactc
    14341 cttcagcaaa atggtgtgct tgaaatgtcc ccccctcatg aaccgaccgg gagcagtagg
    14401 aaagagggcg tcgtgacgtg atccttgttt gccggagaat agtaagcctc ttatcgggtg
    14461 tagttctggt catggttggt tttatgtggc tgaatcaacc cgttggtgga ccagctcagc
    14521 cagttctcgt cgaaatccca ccgggtactc catttacaca ggtttccgac attctcgatc
    14581 aacatcacct tattaaatca ggatggtttt tcactttttt ggggcgagtg caacgggttg
    14641 atcgaaatat tattcctggc gaatatgaac tggatgcggg aatgcaacca actgaattac
    14701 tgaataagct tgtgaaaggt aaagtgtatc aatatgccat cactattccc gaagggttta
    14761 ccgtggcaca aattgcggac atccttgatt acaaacgtct tgctttgaag caggatattt
    14821 ttcgattaag tcgtgacccg gtatttattc aaagtctgaa tgttcatgcg ccaaccctgg
    14881 agggctatct ctttcctgat acctatcatt tttctcgctt tactccacca gagacgatca
    14941 ttcgaacatt tgtaaaccgg tttcatgatg ttctgactcc agaactcaaa gctcggacaa
    15001 cggccatggg gatgaccctt caggaggtgt tgacactagc ctccgtggtg gaaaaggaaa
    15061 cggggctggc agccgaacga ccattggttt caggagtatt tcataatcgc ctccggctag
    15121 gtattccttt acagagcgat cccaccgtta tctacgctct cgaatcattt gatggaaata
    15181 ttcggaagac cgatttgtcg gttgatagtc cctacaacac ctacaaggta cgcggccttc
    15241 ctcctggacc cattgccaat ccgggtttgg cggccattca tgcggcgctc tatccgactc
    15301 agactgattt tgtgtatttt gtgtcgcgga acgatgggag tcatcaattt tctgtcacgc
    15361 tagcggacca taataaagcg gtggatatct atcaacgacg attgatcggt aagcgtgcat
    15421 cgtaagtggg ggattgtgtc attttatggg tattcatgaa acattgagcc gactaggatt
    15481 aacgcttcca gaagcccctt gtcctgtcgg gtcatatgtc ccggctcgtc aggcaggaga
    15541 tttaatcttt gtgagtggtg tgctgccttt ccgtgacggg aaaattgaac aacctggaaa
    15601 actagggcgg gaattgacgg tcaaggaagg ggctgccgcc gctcaattag ccatgctaaa
    15661 tggcttggcc attctccagg agatgatggg agatttttcg tgtctcaatc aggtggttcg
    15721 tctcacgggg catgtggcgt ctgttgaagg ttttgtagat cagcctacgg tgattaatgg
    15781 ggcttcggat ttgttagtca aactgtttgg agataccggg cgtcatgcca gattggccct
    15841 tggggctttc gaattacctc ttcatgctcc aattgaactt gaattgatcg ttcaagtgtt
    15901 tcccccaaaa gccggtcctt aaaacgtatt catcttttaa caagttcaaa gcccgcctat
    15961 gcttttcgtc aggaatcacc cctcctgatc ctgtagcaac ctcatttgaa atttcaccct
    16021 ttgctttggc tcgctttttg aaattgttta gttaggtttt cctcctttgc ggttgtccta
    16081 tctgaaaatt cattttgaaa tggtagaaaa aatttaaatg tggtgtgaga ctttttccct
    16141 tactcatttg gtctctgatg ggcctggcga acgaagaatt ttcataaaaa tcttatgata
    16201 taagtgtgaa tgttgatctt tctccctgag cggttctaca aaattgacgc ctgaaaagat
    16261 cgcttgatat agtcttttgg gcaaccgctc tgacttggtt ccatcccctg tgttggtagc
    16321 cacgaagttg ggttggcttc tcttcccggt tcgatttgaa tgaagaagtc cattgttcat
    16381 acctaattta tttttagaat ctctaagcaa ggtctggtac ttatgaaacg agttcgaatt
    16441 ctcatgagcg tcgctttagg ttttcttctt ttttcatcca tagcccatgc tgaaatgttc
    16501 ccttccgatg gtcctcccgg cacaacagtg acgattagtg gggaaggatt tggagaattc
    16561 cgaaatactc aggctaaccg agtagaattt cagggtgtct cggcgctcat tcaatcctgg
    16621 gaacctgatt ttattctggt caaggttcct ttgcaggctc gttccggccc ggtgatggtt
    16681 ataaacggtt cagcgaaacg ggaagccgga attttttccg tcaccgatgt tcgtatttcc
    16741 agcttgaatc cttcacaagc cgaagcgggt tccttgctca cgattgttgg tgaacacttc
    16801 ggcaacactg ccggatctcg tgatcccaat accatgttcg gggtcaatca agtgataatc
    16861 aatgggattc gcgcagaggt tcgacggtgg cgtcctacca aaattgaagt tctcattccg
    16921 gccaatgcta agactggaga tgtagaagtg cgtttggcct caagcgaccc attaccggat
    16981 ggttcgtgct gtgctcctgt cgagtatgcg gtcagtaatg ccgtccattt gacggtgatt
    17041 cctcctattg cctttgatcc tactgagggt ccgatcggga gtaaggtcgt attgtccggt
    17101 cagggatttg gggaaagccg ccctgaagat ggacgaatat tttttggggg aaagcctgcg
    17161 atcattgcgc aatggtctga tcgaaccatc gtggctcatg tgccgttaaa tgctcaatca
    17221 ggtttcctga tgcttcaacg ggaaggaaag gagcgagaga ttggaacttt taccgtattg
    17281 gacagtcagg tatcagggat gaatcctgct caaggaccaa ttgggacgct tgtgacgata
    17341 aagggaaaaa attttggatt atattcagaa gccggcagca ctgcctatgc ttttgatttc
    17401 ctttccgggg gcaatggggt ggaaattggg ggagtgcctg ctgtcattca ccgatggctt
    17461 gatgaacaaa tcgatgtctg ggtgccctac agcgcgaaaa gtggaccggt gattgtaaag
    17521 cgcggaggag ccatccctaa aactgatggc acctgttgtg aacaacaaga gattgtgacg
    17581 ctaaaagccg gaacttttac cgttgttcag ccggaaattc attcctactc tccgaaagag
    17641 gccggtttgg atgaggtcgt gactattacg ggcaaaggat tcggtgaatt ccttaaaatt
    17701 tcagaagcga ctcgcttatc gctcaaccag tatgctcatg actggaaaat ctacgagcta
    17761 gggcaagatg tgtctcgtag tgaggtattg gtgaatggag ttgccacgca catcgagact
    17821 tggaccgata ccgagatcac gatccgtgtc cctcgccgtc cgtcctatgg ttttggaaat
    17881 ccaaaaggtt ttgaggctga tttgagcaaa ggagaactga ttcttaaacg ggggtcatgg
    17941 gatatgctgg attccggaga atgttgtaca cctaaaaagt atatttcgat cgttgcgggg
    18001 ccatttacga ttttgcaaag aggccttcct aacaagggtt attggaatga aaaaaatggc
    18061 gaacgcagta atgactaatg gcataatttg gatttggaac aatgatttta tgaaaacttg
    18121 ccatgcgtgg aatcaaaagg tgaaaagcct gtgtgcgttg ggagtttttg caagtttagg
    18181 cttttttgta aatcttcagc cagcctttcc tcaatcgcct ccctctgctc tttccctgca
    18241 atcaagtcct accaaggtga cgtttcttgg tgtgcatttc ctgaccgcca gtaagggatg
    18301 ggttgttgga gcaggtggga ccgtactcca taccgatgat ggcggagcct catggaagtc
    18361 gaacccacgc cggactaacg ccttattgac tgcggtgacc tttgcggatg cgatgcacgg
    18421 atggattctt ggacaaaacg gaacagtgtt gcatactgtc gacggtggaa aacagtggat
    18481 tccacaagat agtgggacaa atggaactct ttacgcggct gattttctca caaccgatca
    18541 tgggtgggtg gttggttccc gtggggtaat tctgcacacg gaagacgggg gagagagctg
    18601 ggccgatcaa gtcagtggta cgacggcaga tctcttcggc gtccattttc tgaatgaaaa
    18661 acgggggtgg gccgtgggtg ctcttggtgt catattggcc actactgatg ggggaaagag
    18721 ttgggggctc caatccacca ataatcccgc aacattcttt gatattgtgt ttacggataa
    18781 tgtgtcggga tgggctgttg ggacgcaggg agccatcttc caaacgttga atggtgggga
    18841 ggtatgggtg gatcacactc gaccctgtgg aagtccatgc attaagcccg ctgatttggt
    18901 caatatacag tttttgaatt cactggttgg tcgaattgtt ggtgagcgtg ggacaatctt
    18961 ggagactcaa gacgctggat ttacctggca ggaaattgag ccactaaact ctgaaaccct
    19021 atatgctcaa tcatttcctg atctttctca tggttttgcc gtgggagacc atgggacaat
    19081 aattcatttt gaaaccgcgt caccacgacc ctaattgtgg acttgtccgg cgcgggcatc
    19141 aaagataatt gagtctggcc tggagaatgg ccaatgaagc caggctggca gtttctgctc
    19201 gcaaaattcc cttacctaag gtggcaaaaa ctacatttag agattgagcc gcttgtcgtt
    19261 cttctttcgt ccaaccgcct tccgggccga tgaacaaggt gattccgcct tgaatttccg
    19321 gtggtaagag tatttttgat agcgaaatgt cctccagccg ctcagctaac atgattagta
    19381 tccctccatt ttcccgctgt aaaaattcct gaaatgtctg aatgggaagt atcgtcggta
    19441 cgttccatcg ctcactttgt tgcgctgctt ccaaagcaat gcgttcccat ctttcctgga
    19501 acttgggtgc gtgtactgga gacactcgag gaatgatcct ctctgtcacg agtggcgcaa
    19561 gcgtatggac tccgagttcc gttgcttttt gaataaccca ggccattttc tctcctttta
    19621 aaagagcttg tgccaaggta atgggcggag tggaagaggg tgaggattct tgaatgctta
    19681 gaacattggc gtaaatagcc tgtttggtta tctgatggat tgttgtctgg taccgtcgac
    19741 ctcgatcatc attgagaatc agccgatctc ctggtctcgt tcgtaaactt tttgacagat
    19801 gggtgaataa agggttgcca attatgaccc tttcatcctc aacctgggtt gaatggatga
    19861 aataaactgg catatgttct agacgtaatg gtgagaaaag atcagaggaa ggttaatcga
    19921 acatattctt gactttatcg aacaggcctt ctccttgtgt gtccgtagaa attccgcact
    19981 cctctgcata cgcggtaagg agttcgtgtt gctttggagt cagtttagta ggaatttcta
    20041 ttttagtccg aatcagttga tccccacgag attgggattt aagcccaggg gcacctaatc
    20101 ctttgaggcg gagaatttga tcatgttggg ttcctccagg gatcttcacc atggtggttc
    20161 catttaaggt ggggacttca acttttcctc caagagtcgc tgtgacaaaa tccaatcgta
    20221 agtcataaag gatctcttgg cctttccgtt gaaaatgtgg atggggccgt acactgagtg
    20281 ctacatacaa atcaccaggg ggtccgccat tcatcccatg ctctccttcg tgggaaaggc
    20341 gaagtctcat gccagtttcc accccagctg gaatggtcac cgccaagagc cgttccttgt
    20401 gaacgcgtct ttgccctcta caggttttgc atgggtccgc aatgacttgt cctgatcctt
    20461 ggcattgccc acaggttcga ttgatggtga aaaacccctg ttgcagacga atttgtccag
    20521 ctccacggca tgcagaacag ggtttgacgg cctgatcgga tttcgcaccg gttccatggc
    20581 aggtttcaca gacttcccaa cgaggaattt tgagtttggc ttccttgcca ttgatggcct
    20641 cttcaaagga gatttctaga ttatattgaa gatcattgcc ttgttcggct cgattttgtc
    20701 ctctaccacc accaaagaaa tcttcaaaaa tatcgccaaa cacatctccg aagccgcctc
    20761 caccaaaccc gaacccgtca gctccacctc cttgcccggc ggctgcatgt ccgaacatgt
    20821 cgtatttttt tcggcgatct ggatcgctga gcacttcata agcctctccg gcttctttga
    20881 acttttcttc cgctgttttt ttgtgattgg ggtcggcatg caaatctggg tgatgttgcc
    20941 tggccagctt tctaaagcct tttttgattt cttcttccga ggcattgcgg tccaccccga
    21001 gaatttcgta ataatctcgt tttccggtct gtgccatcag tgttgtccaa aggatggaaa
    21061 aatttggccg taatgtttaa gcatgcacat cagtctgctt gaagaatttt taattcttgc
    21121 ctttatcaac ttcttcaaat tcggcatcca caactttttc ctcattgccg ggactttcgc
    21181 caccggatgg tccagggccg tcctccggtc cggtcccacc tgacgaggcg tcagtagagg
    21241 ctttcttgta catttcttca gctaatttat gcgaagctgt ggtgaggttt tgcatggcgg
    21301 tttttatcgc ttccaggtcg gtctcctcca tggctttacg gagtgcatcc actgccccgg
    21361 tgatattaga cttttccgat tcctcaatct tgtctccatg ttcgcttaaa ttcttttcgg
    21421 tactataaat aagagtatcg gcctggttct tgacttcaac caattctcgc ttctttttgt
    21481 cctcttcggt atgactttga gcttccttaa cgagacgctc aacttcttcc ttacttaatc
    21541 cactcgaagc cgtgatcttg attgattgtt ctttctgtgt cgccatatct ttggctgaga
    21601 catgcacaat tccatttgcg tcaatgtcaa aggtgacttc aacttgggga acgcctcttg
    21661 gagccatagg aagccccact aaatcaaact gtcccaatag cttattgtca ttggccatct
    21721 cccgctcacc ttggaagact ctgatggtca cggcagtttg gttgtcggct gcggtggaaa
    21781 aaatttggct ttttttggtg ggtatcgtgg tattgcgttc gatgagtttc gtgaagattc
    21841 cgcctagtgt ttcaatgccg agcgaaagtg gtgtgacatc taacaacagg acatccttga
    21901 cgtcgccttt cagtactcca ccttgaattg ctgcaccgat tgcgaccact tcatcaggat
    21961 tcacgccacg atgaggttct ttcccaaaaa attgcttgac tcgttcaatg acttttggca
    22021 tccgagtcat cccacccacc aggacgattt cattgatgtc gctggtggtc atatcagcat
    22081 cggcaagggc ttttttgcag ggttccatag ttcgctcaat taaatcattc accagttgct
    22141 cgtattttga tctggtgagc ttaagcacca agtgttttgg tccactggca tcggcggtaa
    22201 tgaagggcag atttatttcg gtttcttggg aggaggaaag ctcgatcttt gcccgttcgg
    22261 ccgcttcttt cagtcgttga agggccattc gatcattccg taaatcgatt ccctggtctt
    22321 ttttgaattc ttcaaccagc caatccatca cacgaagatc aaagtcgtct ccgcccaaaa
    22381 aggtgtcacc attagtggac ttgacttcga agaccccttc tccaatttct agaattgaaa
    22441 cgtcaaaggt tccgccaccc aggtcgtaga cggcaatacg ttcatctttt ttcttatcga
    22501 gcccataggc gagtgacgct gccgtcggtt cattaatgat gcgcaagaca tttaagcctg
    22561 caatctttcc tgcatccttg gtggcttgcc gttggctatc atcaaaatag gcgggaaccg
    22621 ttatgacagc atctgtcacc ttttccccta aataatcttc agccgtttgc ttcatttttt
    22681 gaaggatcat ggctgaaact tctgctggac tatagctttt tcctcgaatg gtgacgtggg
    22741 catcgccatt agaaccttcc gctgttttat aggaaagccg gtttgctgca tttttgactt
    22801 caggagagga atgctttctc cccataaggc gctttaccga ataaatagtg ttttctgggt
    22861 tggtaatggc ttgccgtttg gcaatctgtc cgactaatcg ttcgtctttg tccgtcaggg
    22921 ccacgaccga tggtgtggtc cgtgcgcctt ccgaattggc gatgacagtc ggatctcccc
    22981 cactcatcac agcaacgcaa gaatttgttg ttcctaggtc aatcccaata attttgctca
    23041 tacctgtatt cctccctctt caccagattc ttccgtggaa catggttgtg tcagttctga
    23101 tttttctttc gctacgctta ccatagccgg gcgtaagatg cgatcgtgca ataaatatcc
    23161 tttttgaaat tcttcaatga cagtgtttgg atccacgcct tcggattcca cctgggtcac
    23221 tgcttggtga atagcaggat caaatgaatt tcccgtggta catagttgtc gaacgccaac
    23281 tttacccacg gtctccagga attgtttgtg ggtaagctct accccttcca gtaatggccc
    23341 gctggttcct gtatctttcg cgcactgaat agctcgctca agattgtcta taataggaag
    23401 caaatttttc agtaatgatt cattgccaaa tcgaatcgaa tcgctttgat cccgttgcgc
    23461 ccgacgtttg tagttttcaa actctgcagc cagacgcaaa tatttatctt gggagatttg
    23521 gagttcttct tctggcgttt cgccacattc cgaagtattt cctgttgtgt cctccgttgg
    23581 atcttcttga ggaccgactt cttctgcagt cacttcttca gttgtttcat ctgtggaaga
    23641 catggattta ccttttcccg tttgttgcgt catgaatgac caatatgaag agagagacat
    23701 ttcaagctgc ttatcgaagt tagatagtca cttcctctgg caagtcaacc catcctctta
    23761 aatttcttat gatttttggt ataaagaaaa caatagaaaa tagggagaaa agttatccac
    23821 aggaaggggt catgcgatca tagaggagct ttaaaccctc taatgtaagg tttggtctaa
    23881 cttcctgaat agtccgagaa attggaacaa tcgtctgagc cagtccacct gttgcaatga
    23941 caagcgggga atctccaatt tcatgttgta ttctcctcac gatttcatca accaaaccag
    24001 catatccata cataattcct gcctgcatac tggaggttgt gtctttgcca attacggagg
    24061 ccgggatagc cagatcaact ttgggaagct ttgctgtttt agtgtgaagg gtatctgctg
    24121 cgctttttaa gccaggggca atggttccgc ctaggtactc gccaagttgg gtgacgatac
    24181 agaatgtggt ggcggtccca aagtccacaa tgatcagata tcgtcgatag cgggcaaagg
    24241 ccgcagccgc attgacgaga cggtctgtcc caatttcatc gggattactg tattgaagga
    24301 tgagcccatg aggacatgcg ctcgtgacca taaggggttg ttgtccaagt aaggattggg
    24361 ccaacatgtc aaaaatatgc gtgagtgggg ggaccacact cgtgactata cagcctgaaa
    24421 tggcttccgg caggatttta tgaaattgaa ggagggagcg aagaactatg ctgtattcat
    24481 caggggtctt ggaatggtcg gtggataagc gccagtgtga gatcagcgtg ctgccctgaa
    24541 aaatgccaca cacaatttgt gagttaccaa tgtcgatggc taaaagcata aaaaattgcc
    24601 actcctcttg agccatcctt cagcgatggg tcattgttgg gcggttgtgc gtagatgaag
    24661 gatttcccct gaatgaatat ctcgcatcct agacgattgt tcgtttggat cggaagggga
    24721 ggggatgact cggagttggc catgttctcc tatggaatgg gccagtccct gtagttggct
    24781 tccatcaggg aactgaacct gaatcgtgtg tccaatggta atacaccaat ggttgtattc
    24841 ttcaagtaaa gattggggac cattggacct caggttttcc caattttctt ccaaggatgt
    24901 gatcactttg gtgatcagcg cttctctatc cagagggcaa tggcagtgga tatgaaggga
    24961 agtcgcactg gtttggagtt ctgaggggaa ttcggattga gagaggttaa cgttaatccc
    25021 gaatccgatc acgacgcatg tctggtgtcc tgcattccga aaggaatcac ataacacgcc
    25081 accaagttta cgtcctccta ttaataagtc attgggccac ttaagatcga tacgaattcc
    25141 tgtctgttgt tcaagtgcct gggcaatggc cacgccggcc atgtggggaa tccatcctaa
    25201 atattgaata gttgtttcag gaaccaaaag gagagatccg taaatattgg acttgcctgg
    25261 ggaaaaccat aaccggtcta atcgacctcg ccccgaggtt tgtctgtcgg ccagaacgac
    25321 ggttccagac agtgcctggt ccctggccct ttcaatggca aatgcattgg ttgaagctaa
    25381 ctcgtcaaaa atataaaggg cctcgccaat tttttttaaa ggaagaagtt gggctagttt
    25441 ttgggcggat aacacagatg cgacagtggg ttagagtaaa agtcaggttc ggtattttcg
    25501 tttttgagga acggttttgg ttcgaagcgg agtcatgtcc aaggagaggt ccatggacgg
    25561 ggcggaatgt gtgagggctc caatcgagat gaagttcggt ccggcttctg ccatttctcg
    25621 aatattgtcc agggtcatgc ctcctgatac ttccaccaag gcctgttttt gaatgatgtc
    25681 tatggcctgc ctgacatgat cgggggtcat gttatccaat aaaaggacat cggcttttcc
    25741 cttcaaggcc tgtcggacct gagcaatcgt ttcgacttcc acacaaattc gaaggccatg
    25801 cggggcatgt tgcctggccc atagacaagt ctgggtgatg ttcatacctt gggatgccat
    25861 gaccatcaag tgattatcct taatgagcat accatcataa agggagaagc gatgattttt
    25921 ccctccgcct aggcggacgg cccatttttc tagggcccga agtcccgggg tggttttcct
    25981 tgtgtccact attgcgaccg gataatcgcg aacagcctca caaaattgaa aggtgagcgt
    26041 gctgatcccc gaaagccttt ggagaaagtt tagggcgata cgttcgcctt gaaggagcga
    26101 ctgtgccttc cctttgaggg tgaggacagg ggttgatggg ggcacccttg ttccatcgtc
    26161 aaaatgcgtg gtgaatgcga tagttgaatc gactgcttga aacacctcct tggcaacggc
    26221 cacgcctgcc acgaccatgt ggctttttgc gacaatgtcg gcttgagcta acaaggttgg
    26281 tggaatgaga agggtcgagg tcaggtctcc gtaggcaagg tcttcctcca gagcagcact
    26341 gattacgcgc cgcattgagg aaaggggagg aggcgaggca agagaaggtg cgctcatcag
    26401 gtcttcattg aagccagttg ttgttcggtc atattcagaa ttcccagctc atcgaccagt
    26461 ttggtacgac ggtcttctga atcctggatt acggaaggct ctgccttttc cctgaattca
    26521 gaagaggata accgattgcc aaggcgttgc acttccttct ctttttctcc aatttgtttc
    26581 tggatttttt tgacaacttc atggaggtca atctctctgg gaacgggaat accaatggta
    26641 ctaaaataac ctgaaggcaa ttgtaatgtg cggagagaag gaattccctg gtctgtgatg
    26701 accgatgagc ggagaattga ctcaatataa ggcgcaagaa aggtcagcgt ggccgtatgg
    26761 ttggcctggg tactcgcccc gtagatcaaa ggtgtttgag tggaggaaat attcaggaag
    26821 ttttgtccgg ttctcgccgt gttgacaaac gcttggagaa acaagaaagc ctgttcggct
    26881 tcagcattgg cccattccgg tttttcggtg ggaaagggtt gcgtcatgat tgatatccct
    26941 tcgtgcggga gggattgcca gatttcttcg gtaataaatg gcatcacggg gtgtaacagt
    27001 cgttggatga ttttaaatgt ttccaagagc gtctggcggg tggcgggtgc atcaggatga
    27061 ttttcctgct ggaggcaggg tttggcgagt tctatgaacc aatcacagta ttcatgccat
    27121 atgaattgat agagcgcgga ggcggcttgg tcaaagcgat agtcttcaaa tgctttggtt
    27181 acttcatgga tcgtatggtt cagtcggctg agaatccacc gatccggaaa aggccggagt
    27241 cctgtggaga gtgatacacg tggtccctca gcgtagagat gaataaatcg cgccgcattc
    27301 caaagttttg tgacaaaatt tcgatatcct tcgatgcgtt cttcggcaag cttaatgtct
    27361 cgtcccggtg aggccattga ggccagggta aacctcagcg catccgttcc gtattggcgc
    27421 attttggtca ggggatcaat gacatttcct tttgatttgc tcatcttctg gccctcagcg
    27481 tcccgaacca aggcatggat gtacacatcg cgaaatggga cttttcctgt aaacttcagg
    27541 cccatcatga tcattctggc aacccaaaaa aacaggatgt ccaatcccgt gacgagggtc
    27601 gctgttggat aaaaggtttt cagatcttct gtgttttctg gccatccgag ggtagaaaat
    27661 ggccagagag ctgaggaaaa ccaggtatct aatacgtcag gatcttgaaa aaggttggtc
    27721 cctccacatg ttggacatga ttgaggtggg gttcttgata cgatgggttg agcatctgaa
    27781 gctataaagc aactccttgt gggctgggcg ggaggaataa cctcctggtc aggtgcttcc
    27841 ccagcttggc tggtttttgg caaatggtct gtgttgcagt ttcgacaata ccaggcgggg
    27901 atttgatgcc cccaccagat ttggcgagaa atacaccaat ctttaatctg gcgcatccag
    27961 cccaggtaat tatttttcca ttcatcggga ataatacgaa tgtcacccgc ttcaactgct
    28021 tggatagctg gagtcgccaa gggttgtatc ttgacgaacc attgtgggga aagatacggc
    28081 tcaacaacgg ttttgcatcg gtagcatttg ccaatggcca tggcgtgatc ttcgaccctt
    28141 gagagaaatt cctgtttttc gagggcttct atcacaatgc cccgggcttc ctgcacggac
    28201 ttggagtgga gctccgcttg aagcggggga ttggcttggg cttcctggag ccccatggga
    28261 ctcatacgag cttggtagtc aagtataacc aggcgtgaaa gcccgtgacg ttcgcctgct
    28321 tcaaagtcat taaagtcatg cgcaggggtt atttttacgg ccccagttcc aaattcccgg
    28381 tcaaccaata tcggatcagc cacaatcgga atggttcgtg tcgtcaaagg gacccgaatt
    28441 ttcgttccaa tgtacttctg gtagcgagga tcttcagggt ggacggccac tgcggtatcc
    28501 cccagaatgg tctctggtct ggtggtagcc actatgagaa attgcgtggg atcgtcagct
    28561 agggaatacc gaatgtgata gagctttccc tttgttggtt catgctcaac ttcaatgtcg
    28621 gaaagggccg tcaggcagcg aggacaccaa ttaattaagc gttccccacg gtaaatgagg
    28681 ccttcctcgt acaggcgaac aaacacttct cgaaccgcca gggaaaggcc ttcatccatg
    28741 gtaaaccgaa ggcgagtcca atcacaggag gcccccaact gtttgagttg ttcgataatg
    28801 gtatctccgg attgatgtct ccacttccag acccggtcga tgaaggctgg gcgcccaagg
    28861 tgttctcgtg tttgaccttc ctgcgcgagt tgccgttcaa ccacattttg ggtcgcaatc
    28921 cctgcatggt ctgttccggg aatccataag gtgttcaatc cctgcatgcg tcgccaacga
    28981 acgataatgt cctggataga cgtattgagt gcatgtccta tatggaggga acccgtcacg
    29041 tttggcgggg gaatgaccag actaaaggaa agggcggggt tggttgggtc aggctggaaa
    29101 agttggtgtt caatccagta tgacccccat cggttctcga cttcaccggg attgtacgtt
    29161 ttttctaatt gtgaaaatgc cataatggta tcttgaatgg gcgaaaaaag tccgatgtta
    29221 tcacgggctc tatggccctg caaatacaac ctcttgttcc cctcatgatg cctatggtaa
    29281 aagtggggca caggagtctt aacaatctca attaactcag gagtattgct catgccacgt
    29341 ggtcggcaaa aagatcgtga tttgcgaaga aaacatcgaa aaaatcaaca acgggtaaaa
    29401 ggtttggaag ttgcccggcg cgaaaaaggc aaaaagaaat aataggttaa tttcttgtgg
    29461 tggtcgttga atttttaata gaagattgcc tcacttccta atttccttcc ttgcgtttgt
    29521 tctgattgaa caacttcatg aaacctttgg tttgaggcaa aggtcgggcc accgatcttt
    29581 tagttttgat cctctggtaa ctcatcttac tcctaatgag ttcatctccc ataaaagtca
    29641 ttttttttga tgcggtaggg accttatttg atgtgaaggg atcagtaggg gaggtgtatt
    29701 taacctatgc caaaaaatac ggggttccag acaccgaaca gacccaacag gcattgaatg
    29761 tagcttttaa gcaaacgatg agagagatgc ctcttccggt tttttcggtg gagcggccgg
    29821 aaaaacttaa acaatgtgag cggttatggt ggtttgatgt cgtacatgcc gttttttatc
    29881 gggtgggtat gttcgaggga ttcgatgatt ttttcgaaga ggtatttgag gcgtttgggc
    29941 agtctaccca ttggcaacta ttccctgata catatgaagt cttaggtcat ctaaagcgtc
    30001 agggatttga acttggaatc atttccaatt ttgatagcag gttttttcag gtttcccgtg
    30061 ggcttggcct cgatacgttt tttgattctg tgaccatttc cagcctggtc ggagttgcaa
    30121 agccggccca aaggattttt tgtcatgcac ttgatgaaca catgttagtc cctcaagaag
    30181 ctcttcatgt gggagatcat cctattgagg atttcgatgg ggctcgacag gctggtcttc
    30241 atgctgtctt gattgatcgt tcttcgaatg ccgcctctca tccacacaca ctttcaagtt
    30301 tgacccaact ctcttcatat gatcttttga attcctaaag tacatcccct ccattcaaat
    30361 tttccttaca acactttact attgtgaatc ccatgacaga cctcctatga ggttgttcgg
    30421 tccttttatg atgtcgtggc tattaggtcc tgcctgatta atatttgccc gatgataggg
    30481 tttgtcgtat ggcacctggt gctgccacat gcggaaaatg atcttcaacc atttcgccct
    30541 taacactcgc agtgtgtggt tgtgctgctg cgaccgggcg cgacattgtt gaaaatgaag
    30601 tttcgcccat tcacagcgtc tcagcgactg aaaggccaat taccaaatcg cttgccggag
    30661 gtgcttgttg cctgcgacac agagcctgcc gtgacatcat ttcccactct accccgttaa
    30721 ttgcacatgg ctcgactggg ctcgtttctg gctccacgag gttctaagag acccagggcc
    30781 tcccccagtt cggcccacaa gcgagggatc atggtgcagc tgagccctcc cgacagcgtc
    30841 tgcattcact cagccgctgg gaggttcttg aaaaacgtgg aatctccgtc tgatagtccc
    30901 tcatgaaacg ggaggccacc aacacctgcg cgatcagcat attcaccaag cgagctttgg
    30961 tccgggggac atgctcgggc acggagactt ggggggcttt gaattgggcc caccattcga
    31021 tcgtcctagc cggttcaatc ctttgctcgc gtgcaaaccg cgttcattga ctcggatcaa
    31081 gcgtgatcag cctggtcggg gttggatagt tttttagaaa atccaacgcc agttgtggga
    31141 taaagtcgtc aaagagctca gaagcaatgg catagccgag aagaaactgg accgatgaaa
    31201 gggggcaaga gcttcccagc gcgggtggcc cccggagcca agctgaggag gcgagggggg
    31261 cgtattccgc tcattgctca cagagcccga cgtctggatc aacaccccgg atcccccctc
    31321 ccatatgcag gaaaacggca atcgggacaa acctctgccg gcgcagccat cggaccggaa
    31381 tcggcctcga gtgcggacgc tcattcgcgt caaacaggtc ttcggtcacc acgcgcccgc
    31441 gatggacggc acgctcatcc aaacgagtgg ggggcgagct cgaccaaaga tcggacgtaa
    31501 aaatctgggt gataacgtac aaagggggcc gtttcttacg gttgccagtc ggcagcaggg
    31561 ggcctcatac agggagcggg gaaggcctta aacggtttcc agacaacccc gccctcgcat
    31621 gcacacgatg ctcaggggat tccgcccatc gctcaagcag gcacgggaag ggaggtcccg
    31681 aaactgtgag tttttagggg tgcccttaga taggatgagg taaacgaagg ttataagcat
    31741 atcaagcttg ctagccggtg ataaggagag aattttcggt tttcatgaaa gaccaatata
    31801 ttatgttttg gcttttgtcc cagatcataa cctataaacc atagcaacaa tgctttgagt
    31861 ttttctgctg ttatattcaa acgttaacag tgccaaaatt actgaatact tgaccatttt
    31921 ggccattcag gttacggttt aaaatcaaat tggacccatc aaggctggtg cggcctttta
    31981 tttcatgatg acctctttcc tggtgcggaa tccttaagtt catactcctt cgttaataac
    32041 acgaaacccg tggaattttt tgccatcagc ggattgttaa atggattagc cgctagcggg
    32101 ttggcatcct ttgtctattt tcgcgctccc tatgatccca ggcattggac ttttgggttg
    32161 tttggaatcg ccactgcctt atggagtttt gggtattttg cttggcaagt atcagattca
    32221 gagttttatg cgcttctcaa tctccgcatt ttaatggcag gagccatttt tattcccatc
    32281 accttcctgc atcatgttct gtatttactt gggaaagaaa ttgcttttag tggcaccatt
    32341 aaatggaatt atcttctggg tggggttttt cttcttaccg atttcacgcc tttttttatt
    32401 gaaggtgtcc gtcaaatttc tgtatttccg ttttggggtg tgcctggctt gatctttcat
    32461 ttttgtttga tttggtgggt tggtcttgta atctttgctc attttcttct cactcaagcc
    32521 tatgtgaggg aaaagggatt gcgtcgtagg cagtttttgt atcttctcat cggctctggc
    32581 attggataca ttggtggggc gacaaattat cccctctggt atgggattga aattctgccc
    32641 tacgggacga ttggttttgc ggtatatatt tcaatcgtag cctataccct gttgcggttt
    32701 cactggttgg aattttcggt gtatgttgaa aaaggattgt cctattttgc tattttattg
    32761 tttgtgtccc agcctgttta ccccatgctg ctccttgctc agaaatccct cctgggtgcc
    32821 attaatgtca gattttcagt cgtgcaactt gtgcttcatc tcatgacagt tgtgggtgtt
    32881 tatcagatga aagtaggcac gaaaggcgcc gtcgcgagaa ctattctcaa aggcagggaa
    32941 ttgaggactc aggctctctc cagattttcg tcgaaagtcg caaacattca aaatattgac
    33001 gatttggggc aggccattct agaaaccgtt ggaaggagtg ctggagcatc gaaagccgct
    33061 ctctttgttt tgcaggtcga agaaaatcgg tatagagctg tttcaagctt cggattttcc
    33121 catgatcatc ctgttatcca gaatggatgg gcaatctccg ataatctgcc acaattatta
    33181 ctatttacgc agtcaagggt ctccattaag gagttaaagg gaatagactc caatgaagga
    33241 gagatgcgta tcgctcaaat attagaagaa gccggattgg aggtgtttta tccaattttt
    33301 gggaataacc aattattggg agttttggcg cttggcccca cctccagtga agctattcgg
    33361 atgatgggtg gaaaaacatt ttggaatatg attattcagg aatccgcctt agctttagaa
    33421 aatgccattt tgcgagaaga aattcaccga tctcaaaatt tgctttgcca ggtggatcgt
    33481 ctacggtctc ttgaagccat ggcaaatgga ttaactcaag aacttcataa tccattagtc
    33541 tcgattaaag cctttgttca agtggctgaa ttgcgacggc atgacggaga atttatggat
    33601 aggcttcatc gtatagttgg agaagatctt gcgaagatag atgcattaac aaaagaaatc
    33661 agagaatatg tcaagccgtt atcgggttcc ttaaatgcaa aaacccatgt tcacgatatt
    33721 attgattcct gtctgatgtt tgtggcaagc aatccttctt accaaaagac gatgattgag
    33781 aagatcttta gttccgatgt cccgatggtc tctatagatc gacaagggct tatgcaggcc
    33841 attttcaatg gactgttatt tcttttaaag gatccctcaa aattgtctgg aactttgcga
    33901 attgaaacag aagctgatcg atttgttagg ggacagaatt ggcttcaagt ttcggtgtgg
    33961 tggaaagctg aaaaagcggt aatggattcg gagctggttt caatggagga atggggcttt
    34021 gatgattcct ttattgatga gcctgatcct tccgccaccc aaggattaat cctcgctcat
    34081 caaattattc aacgccattc tggacggttt caggtgtgga ccaataagcg tggcattctc
    34141 ggatttcaaa tccaactgcc tcttagcctc ccctctaaca atgtgtatcc cttgacttcc
    34201 attcgtaatc ccgccattcc tctcaagcca gtgaaggtgt ccccggagtc tgggcctctt
    34261 ttttcgtaga aaaattggta gaataggaac agttgagagc tgactttcgt tgagtcagat
    34321 cgttgaaata gcgtcattct ttgtttatga atagtttttt gcaaaatgag catgccgcat
    34381 ttgaccaggt gccactggtt gtctatcggt gtattcaaca ggggattgac cgaaggtttg
    34441 aatttgtgac cccgtatgtc tattcgttgc ttaacctaac ttctgagcaa cttatccaag
    34501 attctaaagc gtttttttgt cgaatacatc cggaagatcg gaacatgttt ttgagaggtt
    34561 gggagaatca caccaatttg cccgcgacaa ttcggttaga gtatcggatg ctcggtgaag
    34621 ggagtcgggt gatttgggtt caagaaaata gtgtgatatc caaggggagt attgaaaatg
    34681 aaatagtctg ccacggtgcg ttaattgaaa tgccacatca ccagcagatt aaagaggaac
    34741 tgcagcggtg ggaccgggag cttcaatcct tgaccgacaa tcttcccgat attgtcggac
    34801 gatttgatcg caaaaatcgt gtgatgtatt taaatcgttg gtgggatagc gtggaaccat
    34861 ttcccccaga aaagtatttg ggaaagcaac tcatcgagtt gggggtctct caaaaggtag
    34921 cagatatttt tgaggaaaaa attcaatcgg tttgtgcaaa tggcagatcc gattctctgg
    34981 agatttccca tcccactaag cagggattga gaaattttga aattcgattt tgccccgagc
    35041 ctaaaattaa cggacacatt tcaaccgtat tgttgatctg tcgtgatgtg acagatgttc
    35101 gcatggcgga attggctttt agagaaagtg atgaaaaatt tcgccaattg gctgaagctg
    35161 tggatagtgt gttttggatc tgggatgtag acctacaaca aatagtctat gtgagtcccg
    35221 cttataaacg ccattggggg ggagaccccc aaaaactcat gagtaatccg tttgattggc
    35281 tgaccatcgt ctttccagaa gatcagtcca aagttgaaaa tctgttcttg aaaagaattg
    35341 atgtgaaatg cctggatatt gaatatcgaa ttgtcactcg tcacaatgag cttcgatggg
    35401 tccacaaccg aacctttcct gtgaaagatt cattagggag gattcatcgg gtcattggga
    35461 ttgctcagga tgtgacagaa agaaagaaat gggaagagga aagattgagg ggggccaaac
    35521 tcgaatcgct tgggctctta gctggtggac ttgctcatga tttcaataat ttgctgacgg
    35581 caattttagg gcagctgtcg cttgcgaaat tttcattgga ctcaaccaat cctttattta
    35641 atcggatttc tgaggccgaa catgcctctt taagagcgca agatatttca cggcaacttc
    35701 taaccttttc taagggaggg gctccggtaa aaaagacggt ccttttgcaa gaaatcataa
    35761 aagagaatgt ccagttggtg ttgtcaggat ccaatgttcg tccaatcttt aacattgctg
    35821 atgatttgtg gccagtcact atcgatgcag gacaaatttg ccaagtgatt cataatcttg
    35881 tgataaatgc ccgacaggcg atggaaaagg gtggggaatg tatcattcaa gcccataatg
    35941 ttaagggaga tatggtcgaa cgatcgggtt ttggaccaat ggctttacac gatggcgaat
    36001 ggattgaaat ccgatttatg gacaaagggg ttgggatttc taaggaaaat ctagagaaaa
    36061 tatttgatcc atattttacg acaaagtcaa cagggtcagg gctcggtctt gcaacctcct
    36121 actccatcgt taggaatcat ggaggagtgt tggctgtaaa ttcaacgctc gggcaaggta
    36181 gtactttttc cttatttctc cccgctatcc ccatcccaaa aatagcttac gaagtgcctg
    36241 agaggcgggt gaaaatgggc caaggcaaaa ttctgattat ggatgatgaa attcaaatcc
    36301 ggaaagttct tggtgagatg gtggaaacgt gtggatacag ttaccagaca gccaaggacg
    36361 gggaagaagc cttgagggat ttttgtgagg cgtgggaagt tggttctcca ttttcagccg
    36421 tcattcttga tttaaccgtg cccggaggat taggaggaaa agaagtcctc agtcggctgt
    36481 taactataga cccccaagtc aaagctattg tggtgagtgg ttattcaaat gatcctgtcc
    36541 ttgccaatta tcaagaatat ggtttcaagg gtagagtggc aaagccattt aatcttgtgg
    36601 atctgagtgt tgtgctgcat tccgtattag agtaaacgac ctcatgcgcc ttggcctagg
    36661 gtaggtcctc ggtctctgca tcattgttcc tcaaaaaagg ggaatcctca tttgtgaagg
    36721 attcccgctt ttttgtggcc actgatttta gtgatgctct gcctacagtt tacgaaatga
    36781 ggttgtggac ctggtctgct gactaattct tcatgataac caagaataag ttgactcctt
    36841 cgggttcctg ttcaatgcgg atttgattct ctgtaaaccc gcattcctgc gcaagcctac
    36901 gaaggtgcgt tttgcttcga tgaaataaat cccatttaaa aagttccata tagcctcgac
    36961 tgggattgtc agtcgtgaaa tttccaatga ccattttccc atttggtttc agaagagtga
    37021 agaacctttt taaggtacgc ttaaagatat tgtcattgaa atagtcaaat aaaccagccg
    37081 accaaataag gtggtatgtt ttgttcggtg aaaaacgaaa cacattcttg tgggtaaagt
    37141 gaatgtgagc aaggaagtca cgacaaaggt ccttggcata attaatggca ttcgcgtctt
    37201 gatcaacgca atcgatctca agttttgagg tccctagaac cttgagggct tcatacatat
    37261 cccgtccagg gccagaggct aggttgagaa tatggctggt tgaaggggtt tccatttttg
    37321 tcctccataa ttgatcaagg aaaaaagata atcgatttct gactgccttg ggtgcttttt
    37381 gggaatgaaa aaactggtcc cactttgtta aagaagggtt ggaagatgtg tagtaattgt
    37441 atattttttc aatgatttga taatctcctg catatcctaa cggtttatgg tacgcccacc
    37501 cttgaatggt ttcgggggaa agggcttctc cgaaggcaaa tcgaatggat cttatttgat
    37561 cagcggaaaa taccttgtgg atgttttctc ctgaaaattg tccaagaagc gaatttagaa
    37621 tgggatagtc tgttggatca ggtcctcctt tttttgagaa tccggaaatg agttcaatcg
    37681 tgctttggga aagtttttga atttcggtgg cactccttgg gaaaaactgg gaaagtgaga
    37741 tagtgtgtgg aaacatagga aacctccttt tggtgtttga acaaaacaga tggttgaaaa
    37801 ttccctggaa cgtcaaagac ggtagggtgg gggatgcaca gcgtgacaat gggaaaagat
    37861 tgtgaaccga aaaatatctt aatgatcccc tgatgatggg gggactggga aagggtagtt
    37921 cctggtccgg aattcgtccg catagtagga caaagacccg ggattctgaa ggttgaccgc
    37981 taataaagat ttggatattt catcggactt cgtctattct gccaaacctt aaaaaaggaa
    38041 gggatatgtg gagggagagc atatgaaaat ggatggaatg atccaaatta gtggacggga
    38101 cattacccga tcttaaagac gcctgtctag aaaaatttct gtgagttttg agttttccgt
    38161 aaaagttaaa gtaatggatt atactttttg gcctgctggc taaaattgaa tatttggttt
    38221 aatttttgat tccttcgtag tatgaaaaaa tcggcatgat tgagggaagg gcttccgcta
    38281 tggtcgctaa aatccaaatt gtaaaaaata atttttgtcg atggcaaaga tgaaaaaaat
    38341 aacaggtaaa aaaaaatcta atcccagtgt cagggttgtt tatgtcattc ccaaaactaa
    38401 aaaagcacag tttcagaaac gccttctggc gtggtatggg gaattcggtc gggatcttcc
    38461 atggaggaga acatccgatc catataaaat tctggtttct gaggtaatgt tgcaacagac
    38521 gcaagtggac agggttattc ctaaattcca cgagttcctt ggtaaatatc ccactctgca
    38581 ggatctggcg gaagctcagc cagaggatgt tcgaaagacg tggtatcccc tagggtacaa
    38641 tattcgacca tatcgtcttc aaggcattgc ctgtgaaacg attgagcgtt atgggggtgc
    38701 tattccccat aaacctgagg aactcctttc cctaaaaggc attggtcgct ataccgcagg
    38761 agcaatcaga tcttttgcct ttaataaaga tgcgcccatt ttagacacca atgtgatgcg
    38821 ggtattgcat aggatattta ttgggaaagg ggaggctaag aagcaaaagg cggagttatg
    38881 gttactctct gaaaaactca ttccccgagg caaaggatat gattttaacc aggcgttaat
    38941 ggactttggg gccatggtgt gtacggccag gaagccgatt tgtttattat gtcccatgcg
    39001 gaatatttgc cttaccgtct ctttagatga aaagtaatga gcctcaggaa ttgccaccta
    39061 ttgttgtggc ggccgcaatt atctggcaga aagatagggt tctcctgtca aggagaaaac
    39121 cggaatccta tctagggggg ctttgggaat ttcctggggg aaaaaaagaa tttggagaga
    39181 ctttggagga ttgcctgcgc agagaggtga aggaggagtt aggggtagag attagtgagc
    39241 ctgtttggtt ccattctctt caatatcagt atccggaaaa aatggtggag ctgtattttt
    39301 atacctgttc aatcattctg gggattcctc aagcgttaga atccgctgaa attacttggg
    39361 ttcgcaagca tgaattgatt tcctatgagt ttcctcctgc agatattcca gtcataaaca
    39421 aaattatgca aatttgaaat tcagacgaat cagtatattt ccattcatag ccaggtttta
    39481 tgaggacatg aagattgtcc ttaatatcta acttgctcaa gctattcagc caggatggat
    39541 gaaattgtta gggaatggtc tcttgtctcc cccccatggc tgctccttac cctgaagctt
    39601 ccctttttgc cgttgcgaaa tacgaaggat gaatccataa gggaaatgag cggtgaacgc
    39661 tctatcgct
//
LOCUS       DAORAQ010000010        38110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_237_length_38110_cov_65.819209, whole genome shotgun sequence.
ACCESSION   DAORAQ010000010 DAORAQ010000000
VERSION     DAORAQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_38110_cov_65.819209"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..697
                     /gene="flgC"
                     /locus_tag="PKM72_01945"
     CDS             254..697
                     /gene="flgC"
                     /locus_tag="PKM72_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNP59568.1"
                     /translation="MNIDRVFAVVGSALDAQRQRLNIIASNLANAESTRTPEGGPYIR
                     RDVVFQASPAGQQFSNVFANAFGDQSGPSGVRVTDVIQDDRPLRTVYDPSHPDADDQG
                     YLHLPNVNPIEEMVNLMSATRAYEANLAVMETGKTMTLRALEMSR"
     gene            732..1034
                     /gene="fliE"
                     /locus_tag="PKM72_01950"
     CDS             732..1034
                     /gene="fliE"
                     /locus_tag="PKM72_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNP59569.1"
                     /translation="MEYLPIKSIEGISEPFGVTPLKGPAGQDKGFGDLLKNAVESVNT
                     MQHEAGRLEEAVAHGENVNIHQAVIAGEKAGLSFKLLMQVRNKVIDAYQEVMRMQV"
     gene            1053..1175
                     /locus_tag="PKM72_01955"
     CDS             1053..1175
                     /locus_tag="PKM72_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59570.1"
                     /translation="MMTIMGEVRMNETPHRSHRVRIISVRMTPTFLSKELSGTL"
     gene            1180..2772
                     /gene="fliF"
                     /locus_tag="PKM72_01960"
     CDS             1180..2772
                     /gene="fliF"
                     /locus_tag="PKM72_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNP59571.1"
                     /translation="MDSLLGNFQALTLTQRIGVVVVVALALATIPMLMMVGKSPELVT
                     LFSQLNPDDTRAIIQELGKQGAVYEVGDSGNSIKVPAERVHELRLQLASLGLPESAGV
                     GFEIFDRTGLGATPFTQQMNYRRALQGELARTIGQLSQVERVRVHLVMPEKRLFATEQ
                     KPAQAAVVLTLKRGAPLGGSQVQGIVHLVASSVEGLEPSQVTVVDNHGQVLSQNSADS
                     DAQLTASQIETQRRVERDLEQRVQTMLDQVLGRDKSVIRVTAPLEFRQVEITEESFDP
                     NSQVVRSENRSQEKVLESGSSRGGPGVPGVRSNVPSELKAGNGAEQKEAKRKNETLNY
                     EVNRKVSKIIEPTGAIKRLSVAVLVDGTYEGAPGAEGPAAENAAEKKYVPRPEQEIQN
                     LVQIVKKAVGFSEERGDQIEVINVPFESPTILEGEESMTAGVQSFLAVWGGFLKPILF
                     FFLGAMVLWFVVRPMALNLTKPAAAAVVLPQKGLPATVTEYEAEISETPQEQAIKLAA
                     DNPASAAQVIRTWIKEEQGEKV"
     gene            2776..3771
                     /gene="fliG"
                     /locus_tag="PKM72_01965"
     CDS             2776..3771
                     /gene="fliG"
                     /locus_tag="PKM72_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNP59572.1"
                     /translation="MNSQLSGYEKAAILLLAIGESAAVEVLKVLDQKDIRAIGAYLGA
                     LVNVGQDEHRMVLKEFRELAGTSGLSVEGKAYLSKILNAALGKDKARRILSSLNTSEN
                     AGFETLKSLDAASIANLLAGEHPQTGALILANLESDHAAQILSLLPAHKRDAIVYRLA
                     TTEEVSPNMLDELNSVLQEELRLGSSKNAVAIGGTGLVAEILNSVKRNVEGEILTALE
                     GKNPEIAEEIRGKMFVFEDLENIDDRGMQEVLREVSKEELLLALKPIDGPLRDKFFRN
                     MSSRAAESLKEDMETRGPVKLSDVEASQQNIIKTVQRLAGEGRVALGGKGEEQMV"
     gene            3773..4390
                     /locus_tag="PKM72_01970"
     CDS             3773..4390
                     /locus_tag="PKM72_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNP59573.1"
                     /translation="MRSLMEQDDTVKCFQMVELVHKSAKQRAEEAQPHDCTELQRDAF
                     ERGRQDGIAEGRAQCKAQVEEEVKKAIRLANQIGRARLLALEEHERDIVEVALAISRK
                     ILLRECEIDKELVVRQVRQVLGLLPDKTLVTLKVHPQDFTTLEPLQHTLRPEWADGDH
                     LVLEAYEDVDPGGCVVEQAGLLFDARLTQQLALVANEFGLETPQA"
     gene            4387..5730
                     /locus_tag="PKM72_01975"
     CDS             4387..5730
                     /locus_tag="PKM72_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNP59574.1"
                     /translation="MNLATIQQRLDDITPVTITGRVVKAVGLTLEGTGLGASVGQRCH
                     IFSTRGKSMMEGEVIGFREQRVVVMPFGAVRGIAAGNLIRYDPTSPRLLVGPQMLGRV
                     LDGLGNPLDEKGPLSRSRRYALYAPAPAPMLRERIRTPMDLGVRAINALLTCGVGQKL
                     GIFAGSGVGKSVLMGTMCRHTSADVNVIALVGERGREVREFLERDLGREGLRRSVVVV
                     ATSDQSPLVRVRAAFVATAIAEFFRDQGNQVLLLVDSLTRLAHAQREVGLAAGEPPTT
                     KGYPPSVFTLFPQVLERVGPMGTGSITGLYTVLVDGDDLNDPIADSIRSILDGHIVLS
                     RRLAMQGHFPAIDVLQSVSRVMSDITSTVHQDAARFLVQMMAEYRNAEDLINLGAYQL
                     GSNPRLDAAINMKGPIDSFLRQTREEGVGIPESCQSLEALAQQGRRLLERKGKTP"
     gene            5727..6173
                     /locus_tag="PKM72_01980"
     CDS             5727..6173
                     /locus_tag="PKM72_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59575.1"
                     /translation="MNWTTLLRFRKQVEDLAREEVVLAEWEKSQISSKRDDLMVEMEA
                     VATELEQRIRVGIDNMIAEQRYQWMDRISIAIEQQSQHIQEVEDKLADLRATLKKAHH
                     ARRVVELVIAKKEEEVMQKIATQEQEIQEDVTAHAYAISHLEEMTQ"
     gene            6379..6963
                     /locus_tag="PKM72_01985"
     CDS             6379..6963
                     /locus_tag="PKM72_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59576.1"
                     /translation="MLPSRSLIVWWVLALILWATTVGSVQEMPEVGKTNSFENQLVED
                     ERVEEAPSAESTLLEQLQARLKVVEERERALQQREERLTALQQDIEALAARQTKEGKR
                     LVGEASSLAEEQRRYVEQDPALIHLIKIYESMDPEEAALRIAEMREGLALDILASIKD
                     KKAAGVLAGVEPVKAARLSEGLRRYRDIKLNQAN"
     gene            6985..8559
                     /locus_tag="PKM72_01990"
     CDS             6985..8559
                     /locus_tag="PKM72_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59577.1"
                     /translation="MINALPFFFGPSESPPHVPGQTGGLGLDQAKTTENSSNHLFAKI
                     FRDHSGPDSFQNGVSNTTSGKDGGKVSNFELPLGTTAVSGDNLLALYLAGVFAEDGQR
                     VPEEGDGQRITEEADVGKISSIPLAAEGLNGSRSGLAVSQKALFGLLPGNVPQDGPVS
                     GVVVSPHPFLNGVRGGQTGLPLVMGNSPNQIASVGNILVDPSGTDLIAKVDPQQVLRN
                     PLASVAGEAVTEPHTNQGKPGEPFSSLVQGPGPRPSQDSLSPMGLPQESLKPVSNPVN
                     QMLQVPKPFEDTPAGIADVGQLNQNRLKYVSGVSSVLSESTANGKESSVGIPVDLLAD
                     AKVSLTGVGSRDVLEATGKAEGVDSNAGQGLNHGMGNPTHSQSGFQQSSSSSSPGPTV
                     RMPEEPVSNLPGPALQRLQMEVQLSETNRVQIDVGVQHRQVYASLLMDQATLKNLAVQ
                     FVPQLEEQLTQGDMELQEFSAEVRDQHGERESSTRSDGAGTQTSQRGTTFLHQTPGSL
                     SHVGMRVDEQGLHLVA"
     gene            8784..9452
                     /locus_tag="PKM72_01995"
     CDS             8784..9452
                     /locus_tag="PKM72_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNP59578.1"
                     /translation="MEVTPTQAGTNATATGSANVQAAVSALGSDVFLRLLVTQLQSQD
                     PTNPVQNEEFVAQLAQFTTLEQATSTNKLLEKLIGQDTQRTQLDLVNLIGRTVVAQGD
                     TVSIAEDEQPTLAYALSGEARSVIIKVVDSGKQVVRTIESTDIQKAGANQVQWDGLND
                     SGDRVPEGVYQFIVKAVDANEQPVPNFTFARERVMTIAFGTESPVVLQSGKTLHTEDI
                     LSIL"
     gene            9516..10889
                     /locus_tag="PKM72_02000"
     CDS             9516..10889
                     /locus_tag="PKM72_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNP59579.1"
                     /translation="MGITSAFFAATSGINATSRALSEIGNNIANAQTIGYKSRSVSFG
                     DLFGATIGGGGTSTALVEGRGVRVLGIDPSFTQGSLQTTSNALDLAIDGDGFFRVSDA
                     TGNIFYSRAGQFDVDSKGNLVNPAGLRLQGDQADDTGLLSGTVGNISLTTSTQPGRLT
                     SEIAMSGRLAANAPINAYTIAQVLDPQSNKGLFPGNAPVRVFDSQGVGHDLQIYFAKT
                     AANVWEYSVLAQEGEIDLSTLPAANVDTNTHNALVADGTLTYTDEGFLNLESTVNYYN
                     TAGSGLGGGIDFLGKAPNQPIAFDFGTSITEGGAGTNGLLQQGTSLNNNALVLDTLSQ
                     DGFGPGSLAGTSIGEDGIITGRFDNGTTRTLGQVRLTRFINPDGLQPIGRNLFIQSGD
                     SGTPLDGVPGTGAFGKVSASTLEASNVDLGEELVNMITMQRGFQANSRIITTTNDLLG
                     ELVNLAR"
     gene            11292..11786
                     /locus_tag="PKM72_02005"
     CDS             11292..11786
                     /locus_tag="PKM72_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNP59580.1"
                     /translation="MAEDDAKEEAAVKAGAGKKMILIIIAGVLLLGGGGGAAWYFMGG
                     TEEKPAEHDEHAKAETGHEEPGPVMELEPFLLNLADREELRFLKVSIKLELDRAEEKT
                     DYQNKIPAIRDALLVLLSSKESQLLRTVNGKRRVREEIMTRVNGVMSKGKVANVYFTD
                     FIIQ"
     gene            11880..12869
                     /gene="fliM"
                     /locus_tag="PKM72_02010"
     CDS             11880..12869
                     /gene="fliM"
                     /locus_tag="PKM72_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNP59581.1"
                     /translation="MEKILSQDEVDALLRGVSDGGVETEPEDPVDAGLHDGPVSYDLG
                     NQEWVVRGRMPTLDVIHQQFSRGFRISLSDVLRKTAEVTVTNQTVMKFGEFTKRLPVP
                     AYLQIISMDPLRGLAMIATDAATVYLLVDHFFGGAGQTHVKPEGQDFTIIEQRVMKKV
                     MLMGLGNLQKAWDPVQKLLINAVRVEMNPQLAAIVLPADIVIVVTLGIELGNSVGDLH
                     LCLPYTMLEPIRERLQVSFQGDSFETDYGWVKRFSARLKEAYVTMSVCLGETEISVEE
                     LMQFSPGDVIGLNQGTTQLLTARVEGVPKFLGSPGTTKGMQSFQIKDIILARE"
     gene            12931..13356
                     /gene="fliN"
                     /locus_tag="PKM72_02015"
     CDS             12931..13356
                     /gene="fliN"
                     /locus_tag="PKM72_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNP59582.1"
                     /translation="MSEEDNIDMDTFDAEKEMMEALAQEASAKEQSAAAKKQEVAASS
                     SSQAPELMTSHDPNLNRILDIPLVLSAQLGNTRILIKDLLQLGPGSIVELDKLAGEPL
                     EVLVNERLVARGEVVMVNEKFGIRLTDVISPSERVNKLR"
     gene            13437..13871
                     /gene="fliO"
                     /locus_tag="PKM72_02020"
     CDS             13437..13871
                     /gene="fliO"
                     /locus_tag="PKM72_02020"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNP59583.1"
                     /translation="MDLTHEALKMAGTLALVVLVLLGGLAWVRRTFGELPAKNGEPVM
                     RILGGLRVGTGKHIMLVEVAGEVLVLGTTAREMTLLTTIAEADRIDRLRSITNPLVGP
                     LGAWLGQWTRQAFGKSEMGSVTSKPLIRLAQGLARVRGEGKS"
     gene            13868..14647
                     /gene="fliP"
                     /locus_tag="PKM72_02025"
     CDS             13868..14647
                     /gene="fliP"
                     /locus_tag="PKM72_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960008.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNP59584.1"
                     /translation="MINAYCGFRKRRDFRGLLVLSLVMGPSTSALAQVTKDPAISLQV
                     SGLDGTEPWTFGLRILFLLTALTLAPALLMLVTAFTRVVIVLGLLRQALGTMHAPPNQ
                     VMIGLAMFLTLFIMMPVWEQIRVEALNPLLEQTVTQEVALERAKIPLRMFMLKQVREK
                     DLTLFVEMAKVPHPQKPDDVPLHVLVPAFVTSELRTAFQIGFLIYVPFLVIDMIVASI
                     LMSMGMMMLPPIMISLPFKLVLFVLADGWFLVVGALMNSFQ"
     gene            14787..15056
                     /gene="fliQ"
                     /locus_tag="PKM72_02030"
     CDS             14787..15056
                     /gene="fliQ"
                     /locus_tag="PKM72_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNP59585.1"
                     /translation="MTTESVLSIGQEAIQTILLVAGPMLGLSLVVGLCVSAFQAMTQI
                     NEMTLTFLPKVATMFVVLLVTFPWMVEILVGFMTGVWARIPEFAQ"
     gene            15105..15890
                     /gene="fliR"
                     /locus_tag="PKM72_02035"
     CDS             15105..15890
                     /gene="fliR"
                     /locus_tag="PKM72_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNP59586.1"
                     /translation="MFQWDFAIQQVWQFVVVLVRTAVILSALPLLGGQSVPNRIKIGM
                     AVIISILLMPVVTFTLPPNWLEPANLVIALSAELLVGLVLGLALRLLMTAVELAGSVM
                     GFQVGFAMAGVLDPVTQVETPVFGQLLTILATLLYFQVDGHHLVLLALGSSFQLIPPF
                     GAHLSAPLLSDVTGVMQRTYDTGLKLAFPVMAATFLVHAIMGILGRLVPQMNVMLTSF
                     PITISVGLLVLGLGLPFIALVFQDWIVGMETVLWDLVQDLGNG"
     gene            15883..16959
                     /gene="flhB"
                     /locus_tag="PKM72_02040"
     CDS             15883..16959
                     /gene="flhB"
                     /locus_tag="PKM72_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNP59587.1"
                     /translation="MAENKDGADKSEQPSPKRLAEARRKGQVPMTRELPSLFVLLGGV
                     GMISLWAPHAFVQFFDHYRQWLAQAGTLQLDAQSTHVLLFNIAYQAFVPLIPFGLLVG
                     VFAFLAIVLQTGPLWIEKALQPKPSKLNPSNGLKRIFSWKGVVDLLKSLLKLAGVSGI
                     AYLILSHNMLAILQLPLLELTEAVGGVWGILQYIVWSVGGALLLLAIIDLIYQRWQHT
                     EDHKMTKQEVKDESKDVEGDPQIRSRRLSLQRERARQRMLQAVPKADVVITNPTHVAV
                     ALRYERGKMDAPVVVAKGAGFMADKIKQIARHAGVPIVENRSLARGLYKAVKIGQEVP
                     GALYQAAAEVLAYVYRLKKMHEGP"
     gene            17112..19217
                     /gene="flhA"
                     /locus_tag="PKM72_02045"
     CDS             17112..19217
                     /gene="flhA"
                     /locus_tag="PKM72_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNP59588.1"
                     /translation="MAFDSQKLRDELTPAHYGDVLLPVGVVGMLLLMLLPLPSFLLDL
                     FLSFSITLAVLILLVTMHAGRPAEFSVFPSVVLLTTLFRLALNIASTRAILMHGNEGP
                     AAAGQVIAVFGEFVVGGNYAVGIVVFSILVIINFVVITKGATRIAEVAARFTLDAMPG
                     RQMSIDADLNAGMIDEAEARKRREAISQEADFYGSMDGASKFVRGDAIAALLIIFINV
                     IGGLGIGVLEHGMSIVEAAKRFTLLTVGDAIVAQIPALIMSTAAGIIVTRVSTKTSLG
                     RDLTDQIFFNPHLVAAVAGMLVFLGFLPGLPHVAFLMLGAMTAGLAYVLRNQKQEAAR
                     LKSLPKKKDPSTSPSTESVDTVTPPDLMELHVGYGLVPFVDQAQGGELLERIRSIRRQ
                     TALDMGFVVPAVHIRDNLQLKPHEYSILLKGIQVAKGELVPRHLLAINPGHARKGLQG
                     KPGKDPCFGLPSVWIAPSEKERAQMEGYTVVDGPAIIATHLTEFIKSNGHELLGRQEV
                     QNLLDNLAKTYPKVVDDLIPTQITLGAVVRILKNLLRERVSIRDLRTILETVADYTTQ
                     SKDPDTLTEYARQNLSRTISSQYANADGLLQVISLDPMLDRRLMEAMRPSGGEQGGLP
                     PSLLNQVIVSVRQAIERVVSQGYQPVLLCSQSIRSQLYRLVSPGVHALAVLSPNELDP
                     KTQIQAIEVVRMPHEAETV"
     gene            19198..20535
                     /gene="flhF"
                     /locus_tag="PKM72_02050"
     CDS             19198..20535
                     /gene="flhF"
                     /locus_tag="PKM72_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNP59589.1"
                     /translation="MKLKRFEALSLQEALQAVKAELGPEAVIVSSRRIQKGGGLFGLL
                     SQSIIEVTAAVDRSSQAEVEPARVDRSFQSLLAKRFVPDAPTPPPPPVPIKADHGFSE
                     ALQVANALDPMTQHLHAMHQEIQRLREEQQDPERVLGPLRQELEGLRIVVGQVLGSQM
                     DKRVEGLPGDLMDVYQSLVSQGVNPQTAHHLVRAVVETLGTAGIGNREAMKSLLWERM
                     EEAVAVSGPAVSKHYGQKVMMLVGPTGVGKTTTIAKLAGLARQQDDHRRTVLITLDTY
                     RVAAVEQLRVFAKILKIPLEVAVSHKDFMECMGRHQHADLLLIDTAGRSPKDQAGYDE
                     LLAITRGQVHVETHLVLAAPMAELVQMETIRRYQSVPIHKLIITKLDEVSMGGGLYNV
                     LSQTGLPVSYLSAGQRVPEDLEVATRTRLVELAWGQSPGQVLQERVPLTGVAH"
     gene            20535..21467
                     /locus_tag="PKM72_02055"
     CDS             20535..21467
                     /locus_tag="PKM72_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNP59590.1"
                     /translation="MAGSSSAPLLVPGVSVQRRLDQASGLRAAMNSNLQDPSRIQVIA
                     VSSGKGGVGKSNVVANLAVAAARQGRKVLIMDADLALGNVDILLGLTPQYTIENVLSG
                     QRRLEEVLVTGPEGIRLLPATSGGQAFTQLTHEQQLHLQSAFLQLASPPDLLLIDCAA
                     GISANVLYFSLVAHETLVVVSPDPTSLTDAYALIKILSTRYHLRKFRMLVNKVKHSQE
                     GKEVFRKLSLVTDRFLTISLDYLGCVPTDDYLTMAVAQQRAVCDVYPRAPASRAFTSL
                     VKVLNEWRGDCSWQGGFQLFGPAALGATRTVTEG"
     gene            21471..22265
                     /locus_tag="PKM72_02060"
     CDS             21471..22265
                     /locus_tag="PKM72_02060"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNP59591.1"
                     /translation="MKRGAVTTVSERPVSENTAPSIPTKLSLSPQEREALVQEFLSVI
                     KYMAMRMAMRASSGLNVEDLMSAGMVGLLDALTKFDPSREIKFRTYAEFRIRGAMLDE
                     IRAMDWVPRSLRERIGKIQHAANEWTKRKGRPPTEEELAESLGMQAQEVDETLLQAKG
                     AVVLSLDDLGSNDEDSHPILDALADRDQPSPLETLVSEDARQALVDAIERLPERQRLV
                     LTLYYFEELTMKEIGVVLHVTESRICQLHAQAMIRLKALLHTRLGP"
     gene            22544..23752
                     /locus_tag="PKM72_02065"
     CDS             22544..23752
                     /locus_tag="PKM72_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNP59592.1"
                     /translation="MLPMTTGNIPDGGTLTDNFIYTILIVNNEKIMRMVARKQLAKLQ
                     CRLLEASNGDDALALLDQEPVDLILSDWVMPGLDGPGLCEAIKQDERYRTIHFILMTE
                     LDHPTQIAEGLSRGADNFLSKTASGYEIVARVGAGLRARQLMLDLEKINRLLSQKQAE
                     LHSELRSASTFVRDLLPRKGEVVPGVHVEWEFLPSSHLGGDFFQVARWGDDHLGLMML
                     DMSGHGIGPALRAVALSLFFKEEHIQQMFPSYDPGEILTSLNKQYPMSDDGAYFTIWV
                     GVWQSSTRLLRYATAGHPGAIVVKRDGPSVVLGGRSWPIGFSGDEVYGTDSVVLNPGD
                     RMYLFSDGIYEVMNPQEEMWGRKRLQKAFEEFAGQPLQSGLSRIIEGSRTWNAQAGFE
                     DDVALLGVEF"
     gene            23826..24797
                     /locus_tag="PKM72_02070"
     CDS             23826..24797
                     /locus_tag="PKM72_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59593.1"
                     /translation="MKNTFSRILLVDDDESNLDVLVECLKDEPYELVQAHNGEEALAQ
                     LQQDRQGFHAMVLDRMMPGMSGLDLLLHVKREEHLQWLPVVMQTAAGSPQEICEGIEA
                     GVFFYLIKPFDQPLLLRIVNAAVEEGLKWKRIFRNLSQQSRIIELLQQGRFHVRTMEE
                     AYDLAFLLGRACPNSEKVVFGLNELLMNGVEHGNLGIGFDEKTALQASGTWEEEIQYR
                     VGLPEYVHKVVDVLFERCLDCIRVTITDQGSGFDWRRYEQINPDRILESHGRGIAMAK
                     ALSFSRVEYRGKGNQVVCVIKTDTPIGEECISIGSETSGKAHQLEKA"
     gene            25064..25360
                     /locus_tag="PKM72_02075"
     CDS             25064..25360
                     /locus_tag="PKM72_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP59594.1"
                     /translation="MEECQVMPTIPDESILSPLGDLTIFEAAQFHADLVALHQQEGPL
                     KIDLSGVTCMDSSCVQLMVAATRSGRVSVQGYSSRIRDKFEQIGFAQFLPTQES"
     gene            25442..26554
                     /locus_tag="PKM72_02080"
     CDS             25442..26554
                     /locus_tag="PKM72_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNP59595.1"
                     /translation="MCVVMVGTGIGYALGVKSRQTRIHMLEKQLQEGIQAQRRWLENW
                     KAFCQCLAPVFPVFVGQIKAVIQETGQAADGLIQRFQAISRNAREQVEETEALLNMAE
                     GHGGNDDYTVDRILHDTQKTIEMFVNQVTQTTTVTLATVSVMEQALETTSRISEVVEE
                     VEFIADQTRLLALNAAIEAARAGEHGRGFAVVADEVTKLANRSAQASEQIRTLATTVK
                     GTTESAMSELQVLASLDLSETVEAQNKVLEMIKIMGQKNDALKKSVGHNSGRTKELGK
                     DIGQIVMSMQFQDITRQKLEHVYQPLERIHAPLQAVVNDTEGIDMMPQVLEEIRNLEH
                     TYTMESERLTMEAARSGQGTVMVGTGSDGGDNITLF"
     gene            26710..27081
                     /locus_tag="PKM72_02085"
     CDS             26710..27081
                     /locus_tag="PKM72_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59596.1"
                     /translation="MGKTVLVVDDSVSMRQMVSFTLTGAGYEVVEAGDGKEAVDKLNG
                     GAKPNLVITDLNMPNMDGISLIKAVRGMPAHKFTPILMLTTESSEDKKKEGQSAGATG
                     WVVKPFNPEQMLATIKKVLPG"
     gene            27553..29658
                     /locus_tag="PKM72_02090"
     CDS             27553..29658
                     /locus_tag="PKM72_02090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNP59597.1"
                     /translation="MAVDLSRFQESFFTESAEHVETIESGLLALEQRPKDLDLLNRIF
                     RGAHSIKGNAGMFHFTAIAGLTHKMENILDDLRNEKMPVTPHAIDVLLRGLDGLKSLL
                     DAAQGGESADETAIQVVEAELEACQKGSTAHAHEESSSKTALKSSPPQTATWRMIQID
                     WSPLPELFQRGMDPAQIFKELHELGLVKVLQVRTDGLPPLETLDPERCYLSWTVELET
                     DASIGQVEEVFAFVRDGSELTIIDRAPKQAERYKRVGDILVEEGVVTPEQVTESLAKQ
                     KPLGEILVEEKKASPQQVDKALQKQQQLKKNEVASIRVDTEKIDKLINLVGELVITQS
                     MITDLSEKFSIDQLPVLQERITQLERNTREIQERVMSIRMMPIGSAFHRFPRLVRDLA
                     GKSGKQIQLVMSGEETELDKTLIEAIGDPLTHLVRNSADHGLELPEDRLSQGKSARGT
                     IRLHAYHDGGNICVAVEDDGRGLNREKIIKKAVEKGIIADGSNMSDEAVYQLIFRPGF
                     STAESITDVSGRGVGMDVVKRNIEGLGGSVDIQSTMGKGSRLTLKLPLTLAIIDGMTV
                     RVGQDNYIIPLIAVTESIRPKPNELQRIVGKGEVVSLRGEWVPVVKLYEAFNLTPDFT
                     DPSQALLVIVEADGRRLAVLVDELTGQQQVVIKSLEQNFRKVEAISGATILGDGQVAL
                     ILDVPGLAKLARVGHIAAA"
     gene            29795..30334
                     /locus_tag="PKM72_02095"
     CDS             29795..30334
                     /locus_tag="PKM72_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNP59598.1"
                     /translation="MTTATAEPMEQRSIRTTYASDDQQYLTFNLADEYYGVDILKVQE
                     IKGYTTVTRIPNTPDFLKGVLNLRGTIVPIVDLRMKFGMGVTEPTSFTVVVVVNVRNR
                     VMGFLVDAVSDVLDMNAKDIQAPPDMGTSVDVTFIAGIGSAQDRLVTLLDIDRVLTEE
                     EVTVVGKIQDVEKKAEVHA"
     gene            30412..32649
                     /locus_tag="PKM72_02100"
     CDS             30412..32649
                     /locus_tag="PKM72_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNP59599.1"
                     /translation="MNRMVNKIFRLGIATKLVVLFAIFGIVPMAAVGYLGASATGEME
                     TAAGLRVQAMAETIGDKIDRNLFERYGDPQSFGLNRIVGERYHWYSKENNAIASAMNQ
                     YVATSGIYYLTILVDPAGDVMAVNSKDAKGNSIDTTAIYSKKYTDAPWFKAVEAKEFT
                     TRQPFTSPGNDTATGTYIEDVHVDEDVKSVYLGDDGLTLGFSAPVYVDGDLIGYWTNR
                     VKFSLVEEIVQSAYQELKGAGWMGSEISLLGDQGQVIVDFDPSNQGTEDMTHDFEKTL
                     FKLNLADNGVETAKFAVAGKTGYQLALHDEKNIWQMGGYTHLKGALGYPGMNWSVLVR
                     VPKAEATAAAIAFNRDLTITAIICLVLILVVGLWVGRRGANNLVQISEVAQRAADGDL
                     SRRVTVMSNDELGTMGKAMNVMLDNLVRVVSEVRQAAEHVSIASGEITQGNEDLSQRT
                     SAQAGALEETSASMEEMTSTIKQNADNAKQANQLAVAAREVAEKGGAVTDKAVVAMDE
                     INKSSKKIADIINVIDEIAFQTNLLALNAAVEAARAGEQGRGFAVVASEVRNLAQRSA
                     SAAKEIKTLINESVQKVGDGSELVNRSGQTLGEIVNSVKRVTDIISEISAASQEQAAG
                     IDQVNKSVMQMDQGTQQNAALVEEATSASQSMKQQATELLNQVAFFKLEDTEQKRVAG
                     TQPGSGAKVALSSGNNSSAMSKPLHSVKGVPPPKATAKTQPVGVGSSNGHDRRQRQDD
                     FFEEF"
     gene            32838..34646
                     /locus_tag="PKM72_02105"
     CDS             32838..34646
                     /locus_tag="PKM72_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNP59600.1"
                     /translation="MGKVWNSIGIGPKVVMTIMVVFSSLLAGGAYLLGQQLHSDLERV
                     LSDQAFIVQKQIEVTRAYVTKEFVVKAKAAGMNMGVDHSAPDTVPFPATFARETSELL
                     AEEGVFNARIVSLSPLNPKNAPQDAFEKEALAALSAGQKEFTKNDTVNGKMMFRRATP
                     DLATTQACIGCHEGKKIGEMLGAVSVQIPMDGPAAQLQGNLSNMYMGILAVGVLMMGL
                     LYLMIAKLVVKPMKSLESMAERAKEGDLTGRASVNSQDEIGRTSESFNMMFDRVSEVI
                     GSVKTAVSGITTGTSEINAGTSDLAGRTSAQAGALEETSASMEEMTSTIKQNADNAKQ
                     ANQLAVAAREVAEKGGAVTDKAVEAMDEINKSSKKIADIINVIDEIAFQTNLLALNAA
                     VEAARAGEQGRGFAVVASEVRNLAQRSATAAKEIKTLINESVQKVGGGSELVNRSGQT
                     LAEIVNSVKRVTDIISEISAASQEQAAGIDQVNKAVMQMDQGTQQNAALVEQATSASQ
                     SMAQQAEDLLRQVEFFKVQTEFAGQRSEPVAKTGSSVHTQSTSGTGSQYSRSASSMNG
                     KAQGKHSPTKKEVAVGVGSSGTNGHKKAGDDFFEEF"
     gene            34849..35388
                     /locus_tag="PKM72_02110"
     CDS             34849..35388
                     /locus_tag="PKM72_02110"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNP59601.1"
                     /translation="MRRWAAFAMMFVVFMIGFALVVPVGQAADAAKDLAAKYILATAK
                     AARTVYVKGVVADAGKGGMKPDENWVKDDHAMMLPAQFVKELGKELKEFDLSLVGTEP
                     LYASNAPKSAKEKEMLAELAKGKEKVIVGEDGGVTVGMSADYAIADSCADCHNKHPKT
                     TKKDWKKGDFMGAIVVRLK"
     gene            36223..37062
                     /locus_tag="PKM72_02115"
     CDS             36223..37062
                     /locus_tag="PKM72_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HNP59602.1"
                     /translation="MMTTTLSDKEFEQFKHLIYQQVGITLDVPKKTLLVSRLGKRLRD
                     LQLSSYQAYYDCVSGKGGEEELMKLLDLVSTNKTDFYREPVHFDFLRDQVLPEMQSAK
                     TLRIWSSASSSGEEPYTIAMTLSDAIADINRWDIKILASDISTRVLAKASSGIYEEER
                     VSQLPNDLVKRHFLRGKGPQEGKLQVRPQIARLVTFRRINLMDPTFPIRSQLDVIFCR
                     NVMIYFDRPTQAKLMDKFFRYLRPGGYLFIGHSESLQWIDHQFTYLRPTIYQKPVNVK
                     SEA"
     gene            37156..37764
                     /locus_tag="PKM72_02120"
     CDS             37156..37764
                     /locus_tag="PKM72_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59603.1"
                     /translation="MSSTGLMEFGHIRRMQDTRFPYEVAVILPGEYFVSREPKVVYTV
                     LGSCISVCLRDPEAGVGGMNHFMLAAPSNTEGHDNWADSGRYGSFAMEMLMNDIFKRG
                     GKKERLEAKVFGGGKIYDGTIDIGAQNAAWALAFLEQEGLSPIKADVGDVCPRKVYYF
                     TDSGKVLMKKLDRVVASAIAQEEGQYRKKLQHAPVQSDVTLF"
     gene            37885..>38110
                     /locus_tag="PKM72_02125"
     CDS             37885..>38110
                     /locus_tag="PKM72_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59604.1"
                     /translation="MNGKKIRVLIVDDSALIRNVMTEILSQDPEIEVVGTAPDPYVAR
                     DKMKALHPDVLTLDVEMPKMDGLTFLQKIMA"
BASE COUNT         9535 a         8388 c        11008 g         9179 t
ORIGIN      
        1 gtgaatttgg aaaaagaaat gaccctgatg gcggaaaata ccttgatgta tatggcggtc
       61 agtcaatttc taaaaggtcg cttcgagggg tggaatgcgg ctattgatgg aggaaatggc
      121 ggtaggtgac agccgttgaa ctgatagccg ttatgacgct gattccagga aatcagagcg
      181 agggaatcgg gaagttgttg aggatgagaa gtattggaag atctgaaaag ggtgttggaa
      241 aaaggaggct gaaatgaata ttgatcgcgt gtttgcggtg gtggggtctg ccttagatgc
      301 acaacggcag cggctaaata tcattgccag caacctggcc aatgcagaat ccacccgaac
      361 gccggaaggg ggaccgtaca tcaggcgtga tgtggtattt caggcctcac cggccgggca
      421 acagttttcc aatgtatttg ccaatgcctt tggcgatcag tccgggccat ccggcgttcg
      481 ggtgacggat gtgatccaag acgatcggcc gttgaggacc gtgtacgatc ccagtcatcc
      541 cgatgcggat gaccaagggt atttacatct tcctaatgtg aatccgatcg aggaaatggt
      601 gaatctcatg tccgcgaccc gtgcctacga ggcgaattta gcggtgatgg aaaccgggaa
      661 aaccatgaca cttcgtgcat tagaaatgag tcgttaaggt gtcccaatct tgaaggaagg
      721 aggacgatga aatggaatat ctcccgataa aaagcattga aggtatttca gagccgtttg
      781 gggtcacgcc gctcaaagga ccagcgggcc aggacaaggg attcggcgat ctcttgaaaa
      841 acgcggtgga gtcggtcaat accatgcaac atgaggccgg gcgtttagag gaagccgttg
      901 cccatggaga aaacgtgaat attcatcaag cggtcattgc cggggaaaaa gccggtttat
      961 cgttcaaact tttgatgcaa gtgagaaata aggtgattga tgcataccag gaagtgatgc
     1021 ggatgcaagt gtaagggagt attgagttgc cgatgatgac gatcatgggg gaggttcgaa
     1081 tgaacgagac cccgcaccgg tctcatcggg tacgaatcat tagtgtcaga atgacaccca
     1141 catttttatc caaagagttg agcggaaccc tttaatttaa tggacagtct cctcggtaat
     1201 tttcaagcct taaccctgac ccagcggatc ggcgtggtcg tggtggtggc gttagccctg
     1261 gcgactattc caatgttgat gatggtcggt aagtctccgg aattggtgac cctgttctct
     1321 cagctcaatc ctgacgatac gcgagcgatt attcaggaac ttgggaagca aggtgccgtc
     1381 tatgaggtgg gtgacagcgg gaattcaatt aaagttccgg cggaacgggt gcatgaattg
     1441 cgactgcagt tggcctcctt gggacttcct gagtcggctg gtgtgggatt tgaaatattc
     1501 gatcgcaccg gattgggcgc gacccctttt acgcagcaaa tgaattaccg gcgagcgctt
     1561 cagggagagc tggctcggac catcggtcaa ttatctcaag ttgagcgggt acgcgtgcat
     1621 ctggtgatgc cggagaaacg tctgtttgcg acggaacaaa aaccggctca ggcggcggtt
     1681 gtgttgactc tcaaacgcgg ggctcctctt ggaggctctc aagtccaggg aatcgtgcat
     1741 ttagtagcca gtagtgtcga aggattggag cccagccagg tgacggtcgt ggacaatcat
     1801 ggtcaggtct tgagtcagaa ctctgcggat agtgatgctc aactgacggc ctcccaaatt
     1861 gaaacccaac gacgggtgga acgggacctt gagcaacggg tgcagaccat gttggaccag
     1921 gtcctgggcc gggacaaatc cgtgattcgg gtcactgcgc cgttggagtt tcggcaagtc
     1981 gaaattaccg aagaaagttt tgatcccaat agccaggtgg tgcgaagtga aaaccgtagt
     2041 caggaaaagg tgttggaatc cggttcgagc agaggagggc ctggtgttcc cggtgtcagg
     2101 agtaatgtgc caagcgagct caaggcagga aatggagcag agcaaaagga agccaaacga
     2161 aaaaatgaaa cattgaatta tgaagtcaat cgtaaagtga gcaaaattat tgagccgacc
     2221 ggtgccatta aacgattaag tgtggcggtc ttagtggatg ggacctatga aggggctccg
     2281 ggcgctgagg gaccagcggc tgagaatgcg gccgaaaaga aatatgtgcc acggccggaa
     2341 caggaaatac agaatctggt tcaaattgtg aaaaaagccg tcggattctc cgaagaacgc
     2401 ggagatcaaa ttgaagtcat caatgtccca tttgaatcgc ccactatcct tgagggtgag
     2461 gaaagtatga cggcgggggt tcaatcgttc ctggccgtct ggggtggatt cctcaaaccg
     2521 atactctttt tcttccttgg ggccatggtg ttgtggtttg tcgttcgacc gatggcgttg
     2581 aatcttacca agcccgcagc ggcagcggtc gtcctcccac agaaaggatt gcctgccacc
     2641 gtcaccgagt atgaagcgga aatttcagaa acccctcagg agcaagccat taagcttgca
     2701 gcagacaatc cagcttccgc cgctcaagta attcgaacct ggataaaaga agaacaagga
     2761 gaaaaggtct aatccgtgaa tagtcaatta tccggatatg aaaaagcggc tattttatta
     2821 ctcgcaatcg gggaaagcgc cgcggtcgag gtcttgaaag tgttagacca aaaagatatc
     2881 cgggcaattg gcgcgtatct gggcgcgttg gtgaatgtcg ggcaggacga acatcgaatg
     2941 gtgcttaaag agtttcggga attggccgga acatccggtc tgtcggttga aggaaaagcc
     3001 tacctctcaa aaatcctgaa tgcggccttg ggcaaggata aagctcgtcg aatcctgagt
     3061 tccctgaaca cctcggagaa tgccggattt gaaaccttaa aatcccttga tgcggcttcg
     3121 attgcgaatt tattggcggg tgaacatcct caaacgggcg ccttgatcct ggctaactta
     3181 gagtccgatc acgcggctca gattttgtca ttgctccccg cacataagcg ggatgccatc
     3241 gtctaccggt tagccaccac ggaagaagtc tcgcccaata tgctggatga attgaattcc
     3301 gtgcttcagg aggaattgcg tctgggatcg tcgaaaaatg cggtggccat aggtgggacc
     3361 ggtctggtcg ccgaaatcct gaattcggtg aaacgcaatg tggaagggga aatactgacg
     3421 gccctggaag gcaaaaatcc ggagattgcc gaagaaattc gtggaaagat gtttgtgttc
     3481 gaagatctgg aaaatattga tgaccgtggg atgcaggaag tgctgcgaga agtgagcaaa
     3541 gaagaattgc ttctcgccct gaagccgatc gatggtccgc tcagggacaa attcttcagg
     3601 aatatgtcca gtcgcgcggc ggaatccctc aaggaagata tggaaactcg aggaccggtc
     3661 aagctgagtg atgtggaagc ctctcagcag aacattatta aaaccgttca acgcctggcc
     3721 ggtgaaggcc gtgtagcctt gggaggaaaa ggtgaggagc aaatggtcta aggtgcgctc
     3781 gcttatggaa caggatgaca cggtcaaatg ttttcagatg gttgaattag ttcacaaaag
     3841 tgcgaagcaa cgcgccgaag aggcccaacc gcatgattgt accgaacttc aacgtgacgc
     3901 atttgagcgg gggaggcagg acgggattgc cgagggacgt gcccagtgca aagcccaggt
     3961 agaggaggaa gtcaaaaagg ccattcggtt ggctaaccag attggtcggg ccaggctgct
     4021 cgcgttagaa gaacatgaac gcgatattgt ggaggtcgca ttggccattt cccgaaaaat
     4081 cctgttacgg gaatgtgaga tcgataaaga acttgtcgtt cggcaagtgc gccaggttct
     4141 tggactgctc cccgacaaaa cgttggtgac gctgaaagtc catccacaag atttcaccac
     4201 cttagagccc ctacagcaca ccttacgacc ggaatgggcg gatggcgatc atctcgtgct
     4261 tgaagcctac gaagacgtgg atccgggtgg atgcgtggtt gaacaggccg gtctactttt
     4321 cgatgccagg ctgactcaac aactggctct tgtggccaat gaattcggat tggaaacacc
     4381 gcaggcatga acctcgccac cattcaacaa cgtttggacg acattactcc tgtgacaatt
     4441 acggggcggg tggtcaaagc cgtgggcttg actctggagg gaacaggcct gggagcttcg
     4501 gttggtcagc gatgtcatat cttcagcaca cgagggaagt ccatgatgga gggcgaagtc
     4561 atcgggtttc gtgagcaacg tgtggtggtc atgccgttcg gagctgttcg cggcatcgct
     4621 gccggaaatc tcatacgcta cgatccgacc tctccacgat tattggtcgg gcctcaaatg
     4681 ttgggacgag tcctggatgg actcgggaac ccattagatg aaaagggtcc gctctcccgt
     4741 agccgtcgct atgccctcta tgcacctgcg cctgctccca tgctccggga gcgaattcgt
     4801 acgccaatgg atttaggcgt acgagccatc aatgcccttt tgacctgcgg ggtcgggcaa
     4861 aaactcggaa tttttgcggg gagcggagtc gggaaaagtg tcttgatggg aaccatgtgc
     4921 cgtcacacgt ctgcggatgt gaatgtgatc gcgctggtgg gtgagcgagg acgggaggtg
     4981 agggaatttc tggaacggga tttggggcgg gagggactcc ggcgttctgt ggtcgtggtc
     5041 gccacatccg atcaatctcc gttagtccgg gtccgggcgg cttttgtcgc gacagccata
     5101 gctgaatttt tccgggatca gggtaatcag gtcctcctcc tggtcgattc cttaacccgc
     5161 ttggctcatg cgcaacggga ggtgggactg gctgccggag aaccacctac caccaaaggg
     5221 tatccccctt cggtctttac gctctttcct caagttctgg agcgggtggg cccgatgggc
     5281 acaggatcaa ttaccggtct atataccgtg ctcgttgacg gagacgattt aaatgatccc
     5341 attgccgatt ccatccggtc cattttagac gggcatattg tgttgtcccg gcgcttggcg
     5401 atgcaaggac attttcccgc gattgatgtc ctgcaaagtg tgagtcgggt aatgtcggat
     5461 attacctcga ccgtccatca ggatgctgcc aggttcctgg tgcagatgat ggcggaatat
     5521 cgcaatgccg aagatctgat caatctagga gcctatcaat tagggtcaaa tcctcggctt
     5581 gatgcggcga tcaacatgaa agggccgatc gattcctttc tccggcaaac cagggaggag
     5641 ggggtgggaa taccagaaag ttgccaaagt ctagaagcct tggcacaaca gggccggcgt
     5701 cttcttgaga gaaagggaaa gaccccatga attggactac attacttcga tttcgcaagc
     5761 aagtggaaga tctcgctcgt gaagaagtcg tcctggccga gtgggaaaaa agtcagattt
     5821 cctcgaagcg tgatgacctt atggtggaaa tggaagcggt ggccactgaa ttagaacagc
     5881 gtatacgagt gggtatcgac aacatgattg ccgagcaacg ctatcagtgg atggatcgga
     5941 tcagcatagc catagaacaa cagagccaac atattcagga ggtggaagat aagctggctg
     6001 atttacgggc cacattaaaa aaagcccacc atgccagacg cgtagtggaa ttggtgattg
     6061 ctaaaaagga agaagaggtc atgcagaaaa tcgcgacaca ggaacaagag attcaggagg
     6121 atgtgacggc tcatgcctat gccatctctc accttgaaga aatgacccag tagggggcag
     6181 gaaatatgtc aattttttca cctttccatc agcatattcg taatcgagag catagaccaa
     6241 acactggaat gttcaaaaag gccgtttcag gatggctcaa ggcgttttgg gcgggcgaag
     6301 catacggagg agtatgtgag cacggccgag tggcaagaac gcgggtgtcg gcattttcca
     6361 tcactccgga ggggaacaat gttgcctagt cgatcactca ttgtctggtg ggtgttggcc
     6421 ttgattttgt gggccaccac ggtcgggtct gttcaggaaa tgccggaggt aggaaagacg
     6481 aattcatttg aaaatcaatt agttgaagat gaaagggtgg aggaggcgcc tagtgcagaa
     6541 agcacattat tggaacaact gcaagcgcgt ttgaaagtgg tggaagagcg ggaacgtgct
     6601 ttgcaacaac gtgaagagcg gctcaccgca ctccagcaag acatagaggc gttggcggca
     6661 cgacaaacca aagaggggaa acggttagtg ggtgaggcat cctcgctcgc agaagaacaa
     6721 cgtcgatatg tggaacagga tccggcgtta atccatttaa tcaaaattta tgaatccatg
     6781 gatcccgaag aagcggcctt gcgaatcgca gaaatgcgag aagggttagc cttggatatt
     6841 ttggcatcga tcaaagataa aaaagccgcc ggagtgttag cgggagttga accggtgaaa
     6901 gcggcacgat tatctgaagg cctccggcga tatcgggaca ttaaattgaa ccaagccaat
     6961 taacccaaga ctcagaatta aaccatgatc aatgcccttc cattcttttt cggaccttcg
     7021 gagagtcctc ctcacgtacc cggtcaaacg ggtggactcg gcttggatca ggcaaagacc
     7081 acggagaatt cttccaatca tctatttgcc aaaatttttc gcgatcactc ggggccggac
     7141 tcttttcaga acggtgtatc caacacgacg tctggaaaag atgggggaaa agtgtcgaat
     7201 tttgagttgc ctcttggcac gacggctgtg tcaggtgaca atctcttggc tctttatctg
     7261 gcgggggtct ttgctgagga tggacagcgg gtaccggaag aaggggatgg acaacggatt
     7321 accgaagaag ctgatgtagg aaaaatctca tcgattccct tggcggcaga aggcctgaat
     7381 ggaagtcgct caggcctagc cgtgagccag aaggcgttgt tcggactctt gcccgggaat
     7441 gtgcctcagg acgggccggt ttccggtgtg gtggtttcgc ctcacccatt cctcaacgga
     7501 gtacgtggag gccagacagg tttgccgctt gtcatgggga attcaccaaa tcagatagca
     7561 tccgttggga acatactcgt cgatccgagt ggaactgacc tcattgccaa ggtcgatcct
     7621 caacaggttc ttcgaaatcc cctagcatcg gttgctgggg aggctgtgac agagcctcac
     7681 acgaatcagg ggaaacccgg ggaacctttt tcaagccttg ttcagggtcc cgggcctcgt
     7741 ccttcgcaag attctctctc cccgatgggg cttccgcagg agtcactcaa gcctgtatcc
     7801 aatccagtca accagatgct ccaggttccc aaaccatttg aagacacgcc ggcaggtatc
     7861 gccgatgttg gtcagttgaa tcagaatcga ttgaaatatg tcagtggggt ttcgtccgtg
     7921 ctgagtgagt ctacggcgaa cgggaaagaa tcctcggtcg gaattcccgt ggaccttctt
     7981 gctgatgcca aggtgtcttt gactggcgtt ggttccaggg acgttcttga ggcaacagga
     8041 aaagccgagg gagtcgattc caacgccgga caggggctca atcatggcat gggcaatcca
     8101 acccattctc agtccggctt tcagcagtcc tcgtcttctt cctcacccgg accgactgtc
     8161 cggatgccgg aagagcctgt ttcgaatctt cctggtccgg cgcttcaacg cctccaaatg
     8221 gaggtgcaac tttcggaaac caatcgagtg cagattgatg ttggcgtgca gcaccggcag
     8281 gtctatgcca gtttgttgat ggatcaagct accttgaaaa atcttgctgt tcaatttgtt
     8341 ccacaattgg aagaacaact cactcagggc gatatggagt tgcaggaatt ttccgcagaa
     8401 gttcgtgatc agcacggtga acgggagtca agcacacggt cggatggggc aggaacgcaa
     8461 acgagccaac gagggacgac attcctccat cagacaccag ggtcgctttc ccatgtcggc
     8521 atgcgggtgg acgaacaggg tctgcacttg gtggcgtgaa gaaaatggca gcgcgcaggg
     8581 tgagggttaa aacatcagta gtaaggggta aggaatcagg aattatgaga aggcgatcaa
     8641 atacgacgtt cgtttcacgc ctgatgactc ctgcttcatt aattaccgga aaatgagttg
     8701 ccggtgagct gagggtctct cagtctttaa ttacaaatga tgccctcgcg taaaaatggt
     8761 cgaaaaaaca ggagctcata attatggaag tgacgccgac acaagcagga acgaatgcca
     8821 cggccacagg gagcgccaat gtgcaggcgg cggtttcggc actcggatca gatgtttttc
     8881 ttcgcttgct cgtcacgcag ttgcagagcc aggatcccac caacccggtt caaaatgagg
     8941 aatttgtggc gcagttagcg cagttcacca cgttagagca agccacgagt accaataaac
     9001 tcctggaaaa attgatcggc caggatacac aacggacaca attggatctg gtgaatttga
     9061 ttggacggac ggtcgttgcg cagggtgata ccgtcagtat tgcggaggat gaacagccca
     9121 cgctggccta tgccttaagc ggagaggcca ggtcagtcat cattaaagtg gtggactcgg
     9181 gcaagcaggt ggttcggact atagagtcga cagatattca aaaagccggg gccaatcagg
     9241 tccaatggga tgggctgaat gattcgggag accgggtgcc ggaaggagtc tatcagttca
     9301 tcgtgaaagc cgtagacgcc aatgaacaac ctgttccgaa tttcacattc gctcgggaac
     9361 gggtcatgac cattgcattt ggaacagaga gtccggtggt cctgcaaagt gggaaaacct
     9421 tacacaccga ggacattctc tccatacttt gaggtgggtg gttttagggg aaacatcatc
     9481 aggtcggtca atcgagaaac aaggagtaga cgccaatggg aattacctca gctttttttg
     9541 ccgctaccag tgggataaac gcgaccagtc gagcgcttag tgaaatcggc aacaacatcg
     9601 ccaatgcgca gacgatcggc tataagagcc ggtcggtctc attcggagat ctcttcggag
     9661 ccacgattgg tggtggaggg accagcacgg ccttagtgga aggtcgtggc gtgcgggtgc
     9721 tggggatcga cccgagcttt acgcaagggt ctcttcagac gacgtccaat gccctggacc
     9781 tggccattga tggagacgga ttttttcggg tgagtgatgc aaccggaaat attttctact
     9841 cccgtgccgg ccagtttgat gttgattcga aaggaaacct cgtgaacccc gcggggctga
     9901 gattacaggg tgaccaagca gatgatacag ggcttttaag cgggactgtt gggaacatca
     9961 gtttgaccac cagtactcaa cctggaagac tcacatccga aattgcgatg tcaggccgtc
    10021 tggccgcaaa tgctcccatt aatgcctaca ccatcgcaca ggtgcttgat cctcaatcaa
    10081 ataagggcct attccctggt aatgccccag tccgggtatt tgactcccag ggagtcgggc
    10141 atgatttgca aatatacttt gccaaaacgg ccgccaatgt ttgggagtat agtgtgttgg
    10201 ctcaggaagg agaaattgac ctctcaaccc ttcccgccgc caacgttgat accaatacgc
    10261 ataatgcttt ggtcgcagac gggacattaa cttacactga cgaaggattt ttaaatttag
    10321 aatccactgt taactattat aataccgcgg gatcaggttt aggaggtggt atcgattttt
    10381 taggaaaagc accgaatcaa cctattgcat ttgattttgg gaccagcatt acagaaggtg
    10441 gagccggcac gaatggattg ttacagcaag gcacctcttt gaataataat gcgttagtgc
    10501 ttgacacttt atctcaagac gggtttggac cgggatcact cgcgggaacg tctattggtg
    10561 aagacggaat catcacgggg cgcttcgata atgggaccac caggacgttg ggacaggtgc
    10621 gcttaacccg atttatcaac ccggatggac tgcaaccgat tggacggaat ttgtttattc
    10681 aatccggtga ttccggcact cccttggacg gggtgccagg cacgggtgcc tttggcaaag
    10741 tatcggccag caccctcgaa gcctccaatg tggatttggg tgaagagtta gtcaatatga
    10801 ttaccatgca gcgtggcttc caggcgaatt cacgaatcat caccaccacc aacgatctgc
    10861 tcggagaact ggtaaatctg gcccgttaga ttttaaactt ggatgtgact catcaagccc
    10921 ccaggcaggt attcctgtct gggggttttt ttctaatgga ttgttaaaaa aacttgcctc
    10981 aggaacccag aggcggaagg taaaaatctg ttcctttccc ttgcctcctt aggcctcatc
    11041 gctcaccttc cataattgaa cctgtcaaag cttcccccga aatgccagga aaatgacgta
    11101 atggttcgtc tcatcttgtt gccgggcaaa tgagatgcag gcagggccac ggaataaaac
    11161 tatgcgaatt cagtacgtta caggattagc ttcaggctgc ttacgtagaa aaatggagtg
    11221 gcacaaaggt tgctgatttg gaaagaggaa tgtgatgacc cacaaaaatt tctagcgtgt
    11281 gaggttgatg tatggctgaa gacgatgcca aagaggaagc tgccgttaaa gctggggcag
    11341 ggaaaaaaat gatcctaatc attatcgcag gcgttcttct tctgggcgga ggaggaggtg
    11401 cagcctggta tttcatggga ggcacagagg aaaaaccagc cgagcacgat gagcatgcca
    11461 aagccgagac tggtcatgaa gagcccgggc cggtgatgga actagaacct tttcttctga
    11521 atttagcgga tcgtgaagag cttcgatttt taaaagtgag tatcaagctt gagttggacc
    11581 gggctgaaga aaaaacggat tatcagaata aaattccggc cattcgtgat gcgctgttgg
    11641 tgttgctgag tagtaaagaa tcgcaattac ttagaacggt gaacgggaag cggcgggttc
    11701 gagaagaaat tatgacgcga gtcaacggcg tcatgagtaa agggaaagtc gccaatgtat
    11761 attttactga ttttattatt cagtagggcg tggggagaaa gaagtaagcg ttcagggtcc
    11821 gggagttaga aagaggagag aagagacagc agggctgttt ctttgtatca cgtccgaaca
    11881 tggaaaaaat tcttagtcag gatgaagttg atgcccttct tcggggggtt tccgatgggg
    11941 gcgttgagac ggagcctgaa gacccggtag atgccggact acacgacgga ccggtttcgt
    12001 atgatttggg gaatcaggaa tgggtcgtcc gcgggcgaat gccgaccctg gatgtgatcc
    12061 accaacaatt ctcgcgtggt tttcgcatct ctctgagcga tgtgcttcga aaaactgctg
    12121 aggttacggt caccaaccaa acggtgatga agttcgggga gttcaccaaa cgtcttcccg
    12181 tcccggccta tctccagatt atttccatgg accctcttcg cggcttagcg atgatcgcca
    12241 cggatgcggc gacggtgtat ttgcttgtgg atcatttttt cgggggtgcc ggacagactc
    12301 acgtgaaacc ggaagggcag gattttacca ttatagagca gcgcgtaatg aagaaagtca
    12361 tgctgatggg gttggggaat ctccaaaaag cctgggatcc tgtccaaaaa ctcctcatca
    12421 atgcggttcg ggtagaaatg aatcctcagt tggctgccat tgtgttgccg gcggatatcg
    12481 tgattgtggt cacgttaggc atcgaattag ggaattcggt tggagatctc catctctgtc
    12541 ttccctatac gatgttggag ccgatccggg aacgtctaca ggtgagtttt cagggagact
    12601 cgtttgaaac ggattatggt tgggtgaaac gattttcggc caggctgaag gaggcttatg
    12661 tcacgatgtc tgtctgtttg ggagaaacgg aaatctcggt ggaggaattg atgcaatttt
    12721 cacccggcga tgtgatcggg ctgaatcaag gaaccacgca actgttaacg gccagagtgg
    12781 aaggagtccc gaaatttctt ggttctccgg gaaccaccaa aggcatgcaa tcatttcaga
    12841 ttaaggacat catcctggcg agagagtaag ggccggtgaa cacgatgagg agccggccag
    12901 tcactgaccg tggaaacctg gagataaacg atgagtgaag aagacaacat cgacatggat
    12961 acatttgatg ctgagaaaga aatgatggag gcgttagccc aggaagcgtc ggcgaaggaa
    13021 cagagcgctg cggcaaaaaa acaggaggtg gcggcgagta gcagtagcca ggcgccagaa
    13081 ttgatgacgt cccacgatcc gaatttaaat cgcatcttag atattccctt ggtgctttcg
    13141 gcacaactgg gcaacacacg catactgatt aaggacctgt tgcagttagg acccggatcc
    13201 attgtggaat tagataaact cgccggtgaa ccgttggaag tgttggtgaa tgaacggtta
    13261 gtcgcgcgtg gtgaggtggt gatggtgaat gagaaattcg gcatccgtct cacggacgtg
    13321 attagccctt ctgagcgggt gaataaactt cgatagggac atgaggtgta aggatgaaca
    13381 cgtgaagggg cgatgaggcc gtttcacgca tcacgactga cgcatcacga aacaatatgg
    13441 atctcacaca tgaagcgctc aaaatggcgg gtaccttggc cctggtggtc ctggttcttc
    13501 ttggaggatt agcctgggtt cggcgtacat tcggagaatt gcccgcgaag aatggtgagc
    13561 cggtgatgcg cattttgggc ggacttcggg tcgggacggg aaagcacatc atgctcgtgg
    13621 aagtggccgg agaagtgttg gtgttgggaa cgacggccag ggagatgacg ctgctgacga
    13681 ccatagcgga ggccgatcgg attgatcggc tccgctccat tacgaatccg cttgtcggac
    13741 ctttaggcgc ttggttgggt cagtggacgc ggcaggcatt tgggaaatcc gaaatgggat
    13801 cggtgacatc caaaccgctc atccgattgg cccaaggcct cgcaagggtg aggggagagg
    13861 gaaagtcgtg atcaacgcct attgcgggtt tcgtaaacga cgtgattttc ggggactgct
    13921 tgtcctgagt ctggtcatgg gaccatcgac ttcggccctt gcccaggtca cgaaggatcc
    13981 ggcgatcagt ttacaggtgt ccggattgga cgggaccgaa ccctggacct tcgggcttcg
    14041 cattctcttt cttctgacgg ccttaacgct tgcgcctgcc ctgttgatgt tggtgacggc
    14101 atttacacgc gtggtgattg ttctgggtct tcttcggcag gcgttgggga ccatgcatgc
    14161 tccaccgaat caagtgatga ttggattagc catgtttttg accctgttta ttatgatgcc
    14221 ggtctgggaa caaattcggg ttgaggcgct taatccccta ttggaacaaa cggtgactca
    14281 ggaagtggcg cttgaacggg ccaaaattcc actaagaatg tttatgctga aacaagtgcg
    14341 cgaaaaggat ttaaccttgt ttgtggagat ggccaaagtt cctcatcctc agaagcccga
    14401 tgatgtgccg ctccatgtcc tggtgccggc gtttgtcacg agtgaattgc ggaccgcatt
    14461 tcaaatcggt ttcctgatct atgtgccttt tctcgtgatc gatatgattg tcgccagcat
    14521 attaatgtcc atgggtatga tgatgttgcc accgatcatg atctcgttgc cgtttaagtt
    14581 ggtgctgttt gtgttggcgg atggatggtt tttggtcgtg ggagcattga tgaatagttt
    14641 tcaataggaa aggccgtagg acgtgaagcg tcaggcgtgg ggagtgatcg aatgccgtca
    14701 ttgtctttct tctgtctgtc ctcctacagc tttttcttca tgtctaactt tccaaatttc
    14761 atggccagtc tgaaagggtt gaagagatga caacggaaag tgtcttgagc atcggtcagg
    14821 aggcgattca aaccatactg cttgtggctg gtccaatgtt ggggctcagt ttagtggtag
    14881 gactctgtgt gagtgcgttc caagccatga cccaaatcaa tgaaatgacc ctcacgtttt
    14941 tgccaaaggt tgccaccatg tttgtggtgc tactggtcac ctttccctgg atggtggaaa
    15001 ttcttgtggg attcatgaca ggggtatggg caaggatacc cgagtttgcc caatgataga
    15061 gggagtcaac ttacggcatg tgtgagaaag atgtccggta accggtgttt caatgggatt
    15121 ttgcgattca gcaggtgtgg caattcgtgg tcgtgctcgt ccgtacagcc gtcattttat
    15181 cggccttacc gttgctgggt gggcaatcgg ttccgaaccg catcaaaatc gggatggcgg
    15241 tcattatctc catcctgttg atgcccgtcg tgacgtttac tctccctccc aattggttgg
    15301 aacctgccaa tctggtaatc gcgttgagtg ccgaattgct cgtcggattg gtcttaggtt
    15361 tggcattgcg tttacttatg accgcggtgg aactggctgg gagtgtgatg ggttttcagg
    15421 tggggtttgc catggccgga gtcttggatc ctgtcacgca agtggaaaca ccggtgtttg
    15481 gtcaattact gaccattctg gcaacccttc tgtactttca agtcgatgga catcatttgg
    15541 tgttgttggc gttgggcagt agttttcagt tgattccccc cttcggcgct catttaagcg
    15601 cccctctgtt gagcgatgta accggagtca tgcaacgaac ctacgatacg ggattgaagc
    15661 tggcctttcc ggtcatggcc gcaacatttc tggtccatgc catcatgggg attcttggcc
    15721 ggctggttcc tcagatgaac gttatgctca ccagttttcc cattacgatt tctgttggat
    15781 tgttggtgtt aggactgggt ttgcccttca tcgccttagt ctttcaagac tggattgtcg
    15841 gcatggagac ggtcttgtgg gatttagttc aggacttagg aaatggctga gaataaagac
    15901 ggggcggata aatcagagca accgagcccg aaacgcttag ccgaggcccg tcgcaaaggc
    15961 caagtgccga tgaccaggga attgccctcc ttgtttgttt tgttgggagg ggttgggatg
    16021 atttctctct gggctcctca cgcgtttgtc cagttttttg atcactaccg acagtggttg
    16081 gcgcaggccg gaaccctcca attagatgct cagtccacac atgtgttgtt gtttaatatc
    16141 gcctatcaag cctttgttcc tctcatcccc ttcggactcc tggttggtgt gtttgcgttt
    16201 ctcgctattg tgttgcaaac cgggccgctt tggattgaaa aagccttgca acccaaaccg
    16261 tcgaaattga atccctctaa tgggttgaag cgtatttttt cgtggaaagg ggtcgtcgac
    16321 cttttgaaat cattgctgaa gctggcaggc gtgagtggga ttgcctatct gattctgtct
    16381 cataacatgc tcgcgattct tcaacttccc ctccttgagc taaccgaggc ggttggtggt
    16441 gtgtggggga tcctgcaata cattgtgtgg tcggtcggag gggccttgct cctgttggcc
    16501 atcatcgatc tgatctatca gcgctggcag cataccgaag atcacaaaat gaccaaacag
    16561 gaagtgaaag atgagtcgaa ggatgtggaa ggtgatccgc agattcgatc ccgccgcctc
    16621 agtttgcaac gggaacgtgc cagacagcgc atgttacagg ctgtgcccaa agccgatgtg
    16681 gtgattacca acccaaccca cgtggctgtg gcgctcaggt acgaaagggg aaaaatggac
    16741 gctccggtgg tggtggccaa aggcgcaggg tttatggcgg acaagattaa gcagattgcg
    16801 cggcatgcgg gggttcccat tgttgaaaac cgcagtctgg cccgcgggtt gtataaagcc
    16861 gtgaaaatcg gccaggaagt gcccggggca ttgtatcagg cggcggctga ggtgctggcc
    16921 tatgtgtatc gcttgaagaa aatgcatgag ggaccgtgac gcgtaaggtc taacgcatga
    16981 gggggagaat cgaaaaagga aatatcggca tgccccctca cacttcacac ctgccgtctc
    17041 atgacgtgta ggagaggcat atgaaaatag agtaccaacg aatgctgcaa cggtaaccga
    17101 tcaaggcaca catggcgttt gattctcaaa aattacgtga cgaactcact cccgcgcatt
    17161 atggggatgt cctcttgccg gtcggggtgg tggggatgct cctcctcatg ctgttgcccc
    17221 ttccctcgtt tcttctggac ctctttttat cctttagtat taccctggct gtcttgattt
    17281 tgctcgtgac catgcatgcc gggcgtcccg cggaattttc cgtcttcccg tcggtggtat
    17341 tgctcaccac gttattccgg cttgcgttga atattgcttc gacgcgggcc attcttatgc
    17401 atgggaacga aggtcccgct gccgccgggc aagtcattgc cgtgtttggg gaatttgtgg
    17461 tcgggggaaa ttatgccgtg ggcattgtgg tcttctccat cctggtgatt atcaattttg
    17521 tcgtgattac caaaggtgcc acccggatag ctgaagtggc cgcgcggttt accctggatg
    17581 cgatgcccgg caggcaaatg agtatcgatg cggatctcaa tgcgggaatg attgacgaag
    17641 ccgaagcccg gaagcggcgg gaggccattt cgcaggaagc tgacttttat ggatcgatgg
    17701 acggtgctag taaatttgtt cgcggcgatg cgattgccgc gctgctcatt atctttatca
    17761 acgtcatcgg cgggttgggc atcggcgtgt tggaacatgg gatgtccatc gtggaagccg
    17821 cgaaacgctt taccctgctg acggtcggcg acgcgatcgt ggctcaaatt cccgccctga
    17881 ttatgtcgac tgccgccggt atcattgtga cacgggtttc gaccaaaacc agccttggcc
    17941 gtgatttaac cgaccaaatc ttttttaatc cccatcttgt ggccgccgtg gccggcatgt
    18001 tggtcttttt gggatttctt cccggattgc cgcatgtcgc gtttttgatg ttgggagcga
    18061 tgaccgccgg cttggcttat gtattgagga atcaaaagca agaagccgct cgcctcaaaa
    18121 gtctccccaa gaaaaaagat ccatcgacat cccctagcac ggaatcggtg gataccgtca
    18181 ctcctccgga tctgatggag ttgcatgtcg gttatggact tgtcccgttt gtcgatcagg
    18241 cccagggagg ggagttgcta gaacggattc ggtccatccg gcgccaaacg gctcttgata
    18301 tgggatttgt cgtgcccgcc gtgcatatcc gggataacct ccagctcaaa ccgcatgagt
    18361 acagtatttt gttgaagggg attcaggtgg ctaaggggga actcgtgccc aggcatttat
    18421 tggccatcaa tcccggtcat gcaagaaagg gcctacaagg taagccggga aaagatccgt
    18481 gtttcggttt gccgtcggtc tggattgccc cctccgaaaa ggagcgagct caaatggagg
    18541 gttatacggt cgtcgatggg ccggccatta ttgccactca tctaacggaa tttattaaat
    18601 ccaatgggca tgaattgctg ggtcggcaag aggtccagaa tttgctggat aacctcgcta
    18661 agacgtatcc caaggtggtg gatgacttga ttccgactca aataacgtta ggagcagtgg
    18721 tgagaatcct taagaatctc ttacgagaac gagtgtcgat ccgcgacctg cggaccattt
    18781 tggagactgt tgcggattac accactcaga gtaaggatcc tgacaccctg acggaatatg
    18841 ctcggcaaaa tttatccaga accatttcgt cccaatatgc caatgcggac ggattgctgc
    18901 aggtgataag tctggatccg atgctggatc gccggttgat ggaggcgatg cggcctagtg
    18961 gtggagaaca agggggattg cctcccagtt tgttgaatca agtgattgtg tccgtccgtc
    19021 aggccattga gcgcgtggtc agtcaagggt atcaacccgt gttgttatgt tcccaatcga
    19081 ttcgatccca actctatcgg ttagtctcgc cgggtgtgca tgctctggcg gtgctctcgc
    19141 cgaacgagtt ggaccctaaa actcaaattc aagctattga agtcgtgagg atgccacatg
    19201 aagctgaaac ggtttgaagc cctctcatta caagaagctc tccaagcggt gaaagcggaa
    19261 ctgggtcctg aggcggtcat cgtttcgtcc cgccggattc aaaaaggggg aggcctgttt
    19321 ggtctgctca gtcaatcgat cattgaggtg acggcagcgg tggatcgttc atctcaggcg
    19381 gaagttgagc ccgcacgtgt tgatcgatct tttcagtccc ttctcgccaa acgctttgtg
    19441 cctgatgcgc ctactcctcc acctccaccg gtgccgatca aagccgatca cggattttcg
    19501 gaagccctgc aagtcgcgaa tgcgctggat ccgatgactc agcaccttca tgcgatgcac
    19561 caggaaattc aacgattgcg cgaagaacaa caagatcctg aacgggtgtt ggggcccttg
    19621 cgacaagagt tagaaggctt gcggattgtg gtgggacaag tgttgggaag tcagatggac
    19681 aaacgggttg aaggcttacc tggagacctg atggacgtct atcagtcctt ggtgtctcag
    19741 ggagtgaacc ctcagactgc gcatcatctg gttcgtgcgg tggtggaaac gctcgggaca
    19801 gccggtattg gcaatcgcga ggcgatgaaa agtctgttat gggaacggat ggaggaggcg
    19861 gtggccgttt cgggtccagc ggtgtctaag cattatggcc aaaaagtgat gatgctggtt
    19921 gggccgacag gcgtggggaa aacgacgacc attgccaagt tggcggggtt ggctcgtcaa
    19981 caggatgacc atcggcgaac ggtgttgatt acgttggata cgtatcgtgt ggcggcggtg
    20041 gagcaacttc gggtatttgc caaaatttta aaaatacctc tggaggtagc ggtctctcac
    20101 aaagatttca tggaatgcat gggacggcat cagcatgcgg atttattgtt gattgatacg
    20161 gcgggacgaa gccccaagga tcaagccggg tatgatgaat tactcgcgat caccagggga
    20221 caagtccatg tggaaacgca tctggtctta gccgccccca tggccgagtt ggtccagatg
    20281 gagaccattc ggcgctatca atcggtccct attcataagc tcatcattac gaaactggat
    20341 gaggtgtcga tggggggcgg tctgtataac gtgttgtcac agacgggttt gccggtctcc
    20401 tatctttctg ccggacagcg tgtacctgaa gatttagagg ttgccacccg aacgcgcctg
    20461 gtggagttgg cctgggggca atcaccgggg caggttcttc aggaacgtgt accgttgacg
    20521 ggggtcgcgc actaatggcc gggtcatcat ctgcaccact cctggttccc ggtgtttccg
    20581 ttcaacgacg actggatcaa gccagtggat tacgtgccgc catgaactcc aacctacagg
    20641 atccttcacg cattcaggtg atagcggtca gttcaggaaa aggcggggtc ggaaaaagta
    20701 atgtggtggc gaatttagcg gtggctgcgg ctcgtcaggg acggaaggtg ttgatcatgg
    20761 atgcggatct tgccttagga aatgtcgata tcttattagg actgactcca cagtatacga
    20821 ttgagaacgt gctttccggg caacgacgct tggaggaagt cctggtgacc gggcccgaag
    20881 ggattcgcct gttgccggca acgtccggag gtcaggcctt cactcaactg acgcatgagc
    20941 agcaactcca tttacaatcc gcctttcttc agttggcgtc tccgccggac ttgctgctga
    21001 ttgattgtgc ggcaggaatt tcggccaatg tgttgtactt tagtttagtg gctcacgaaa
    21061 cgctggtggt ggtctcgccc gatccaacct ctctgactga tgcctatgcc ctgatcaaaa
    21121 ttctttccac tcgctatcac ttgcggaaat tccgcatgct tgtcaacaaa gtcaagcata
    21181 gccaggaagg caaagaggta tttagaaaat taagtttagt gacggatcgg tttttgacca
    21241 tttcgctcga ttatctggga tgtgttccca cagatgacta tctgacgatg gccgtggctc
    21301 aacaacgtgc ggtgtgtgat gtttacccgc gcgctccggc gagccgggcc tttacttctt
    21361 tggtgaaggt tctcaatgag tggaggggag attgttcctg gcagggggga tttcaacttt
    21421 ttggccctgc tgccttggga gccactagaa ctgtgacgga aggataacgc atgaaacgtg
    21481 gcgcagtaac aacagtgtca gaaagaccgg tttcggaaaa cacagcaccg tcgattccga
    21541 cgaagctttc gctctccccc caggaacgcg aagcgctggt tcaggagttt ttgtcggtca
    21601 ttaaatacat ggcgatgcga atggccatgc gagcctcgag cgggctcaat gtggaagatt
    21661 tgatgagcgc cggcatggtc gggcttctgg atgccctgac gaagttcgat ccctcacgag
    21721 aaattaaatt tcgaacctat gccgagtttc gtattcgtgg cgcgatgctt gacgaaatcc
    21781 gcgcgatgga ctgggtgcca aggtctctta gggagcgaat cgggaaaatc caacatgcgg
    21841 ccaatgaatg gacaaaacgc aaaggtcggc cacccacaga agaagagttg gcggaatcct
    21901 taggtatgca ggcgcaagaa gtggatgaga ctcttttgca agccaaaggc gctgtggtac
    21961 tgagtctcga tgatttgggc tccaatgatg aagattccca tcccattttg gatgcgttgg
    22021 cggatcgcga tcaaccttcg ccgttggaga ccctggtgtc cgaggatgca cgccaagcct
    22081 tggttgatgc cattgagcga ttgcctgagc gccaacgcct tgtgttgacc ctctattact
    22141 ttgaagagct gacgatgaaa gagatcggtg tcgtcttgca cgtcacggaa tcccgcattt
    22201 gtcagttgca tgcgcaagcc atgattcgtc tgaaggctct tctccataca cggctcggtc
    22261 cgtaattaat ttctgtctct gaggaaagcc ctctaggcgg aaacctgtat gcggtttgtc
    22321 catgggctta ggccccgcat cgtaacggag gttttaccct gtggacgtca tgtccatgcc
    22381 gggaaccctt gtatcttcga cctatcggat gccgcacatt gatggtggtg aggaaagctc
    22441 cctgcttccc catgaagggc catcatcaga gttggatttg tgcccatccc tggcgaaaaa
    22501 cacaaatatt taacatcgtc caataacttc attgagaaag atcatgctac cgatgacgac
    22561 cgggaatatc ccggatgggg gaacccttac ggataatttt atctatacca ttctcattgt
    22621 gaataatgaa aaaattatgc ggatggttgc caggaagcaa ttggccaaac tgcagtgtag
    22681 attattggag gccagtaatg gagacgacgc tctggccctg ctggatcagg agccggtgga
    22741 tctcattcta tctgattggg tgatgcccgg tcttgatggt cccggacttt gtgaagcgat
    22801 taaacaagat gaacgctatc ggacgatcca tttcatcctg atgacggaat tggaccatcc
    22861 gacgcagatt gccgaaggac tcagtcgggg ggccgataat ttcttatcca aaaccgcttc
    22921 cggttatgaa attgtcgcca gagtaggcgc gggtcttcga gctcgacagt tgatgctgga
    22981 tctagaaaag atcaatcgtt tgttgtcgca aaagcaagcg gaattacatt ctgagctccg
    23041 ttcggcttcc acctttgttc gagatctcct cccgcggaaa ggggaagtgg ttcccggagt
    23101 tcacgtggag tgggaatttt tgccttcctc gcatttggga ggagatttct ttcaggttgc
    23161 ccgatggggg gatgatcacc ttggtctcat gatgctggat atgtcggggc atgggatcgg
    23221 gcccgcctta cgggctgtgg cgttatcgtt gtttttcaag gaagaacaca tccaacaaat
    23281 gtttccctca tacgatccgg gcgagatcct gacgagtctg aacaaacaat atcccatgag
    23341 cgatgacggg gcgtatttca caatctgggt tggggtgtgg caaagctcca cgcgccttct
    23401 tcggtatgcg actgccggac atcccggggc cattgtggtg aaaagagacg gaccttcggt
    23461 tgtgttggga gggcgatcct ggcctatagg tttttcgggt gatgaggtgt atggaacgga
    23521 ttccgtcgtt ttgaatcccg gagaccggat gtatcttttt agcgatggta tttatgaagt
    23581 gatgaatcct caggaagaga tgtggggaag aaaaaggctt cagaaagcat ttgaagagtt
    23641 tgccggtcag ccgttgcagt caggattgtc ccgtattatt gaggggagcc ggacatggaa
    23701 tgcccaagcc ggctttgagg atgacgtggc cttactcggg gtggagtttt aggcgtgaga
    23761 gagtaagacc tgaggagttt attcaccctc acccctcacg tttggtcgtt gtcgagcgca
    23821 cacgcatgaa aaacacattt tcccgtatct tattagtaga cgacgatgag tcaaacctgg
    23881 atgtccttgt tgaatgtctt aaggatgaac cctatgaact tgttcaagcg cataacggcg
    23941 aagaggcctt agcgcaatta caacaggacc gacaagggtt ccatgccatg gttctggatc
    24001 gaatgatgcc ggggatgagt ggccttgatc tgttgttgca tgtgaaacgc gaggaacacc
    24061 tccaatggct tccggttgtg atgcaaaccg cagcgggatc cccccaggaa atatgcgaag
    24121 ggattgaagc gggggtcttt ttttatttga tcaagccctt tgatcaaccg ttgttattgc
    24181 ggattgtgaa cgcagcggtg gaagaggggt tgaaatggaa acggatattc cgaaatctta
    24241 gccagcagtc ccggattatt gaattattac agcaaggccg tttccacgtt cgaaccatgg
    24301 aagaagccta tgatctcgcc ttcttgttgg ggcgagcctg tcccaattca gaaaaagtcg
    24361 tgttcggatt gaatgaattg ctgatgaatg gagtggagca cggaaatttg ggaatcgggt
    24421 tcgatgaaaa aaccgctctt caggcaagtg gaacctggga ggaagaaatt caatatcggg
    24481 tgggacttcc tgagtacgtc cataaagtgg tcgacgtact gtttgaacga tgcttggatt
    24541 gcatccgggt gacaataacc gaccagggtt ccggcttcga ctggcggagg tatgaacaga
    24601 tcaaccccga ccggattttg gaaagtcacg gacggggtat tgcgatggcc aaagccttaa
    24661 gtttctcacg agttgagtat cgggggaaag gcaatcaagt cgtgtgtgtc atcaaaacag
    24721 acacaccgat cggcgaggaa tgtatctcaa ttggctctga aacctccggg aaggctcacc
    24781 aattggaaaa ggcatgagga gtaaagcgtg aaagtaaggc atgcggaaaa aatgattttg
    24841 tctttcggac attctttttt cctatcttct ttcactgcgg gttgtggcca caataaagga
    24901 atgtggagtc tttctaaagt agggtccttt gggttccgat agtcctgttt gtcccctgtt
    24961 tgtttttgac catgtcaaag gcgctcaggc gggaaactaa agcatcaggc caggaaagtc
    25021 gtctgaatat tcacgttttt tccccgagag atgagaggat aacatggaag aatgtcaagt
    25081 catgccaacg attcctgatg agtcgattct cagtcctctg ggcgatctca ccatttttga
    25141 agcagctcaa tttcatgccg atctggtggc cttgcatcaa caagaagggc ccctcaaaat
    25201 tgatttaagc ggggtgactt gtatggattc ctcctgtgta cagctgatgg tggcagcgac
    25261 ccgatccggg cgggtatccg tgcaagggta ttccagtcgt attcgtgaca agtttgagca
    25321 aattggattt gcacagtttc tccccaccca ggagtcgtaa tacagagcca ataaattatg
    25381 caaatgataa tcatcgagat tcaaaatgag gtgacgtgtg aatggtgatt ggatcgtatg
    25441 gatgtgcgta gtgatggtcg gaacagggat cggatatgcc ttgggcgtca aaagccgcca
    25501 gacgcgtatc cacatgttgg aaaagcaact tcaggaaggt attcaggccc agcgccgctg
    25561 gctggagaac tggaaggcct tttgccaatg tttggccccg gtgtttccgg tatttgtggg
    25621 acagattaaa gcggtcattc aagaaacggg acaagcagcc gacggcttga ttcagcgatt
    25681 tcaggcaatt tcacggaatg ccagggaaca ggtagaggaa accgaagcgc tattaaatat
    25741 ggccgagggg catggcggga atgatgacta taccgtcgat cggattcttc atgacacaca
    25801 aaagaccata gaaatgttcg tcaaccaagt cacacagacg accacggtga cgctcgcaac
    25861 ggttagcgtg atggagcaag ccttggaaac cacttctcga atttcagagg tggtggagga
    25921 agtcgaattc attgccgatc aaacacgatt gttagcactc aatgcggcga tcgaagccgc
    25981 acgggcggga gagcatggac gagggtttgc cgtggtagcg gacgaagtga cgaaattggc
    26041 gaatcggtct gctcaggcct cagagcaaat tcgaaccctt gcgacgacgg tcaaaggtac
    26101 aaccgaatcc gcaatgtcgg aactccaggt gctggcctca ttagacttat cggaaacggt
    26161 agaggctcag aacaaagttc tggagatgat caagattatg ggtcaaaaaa atgatgccct
    26221 aaaaaagagc gttggacaca attcggggcg aaccaaagaa cttggaaagg atatcggaca
    26281 aattgtgatg tccatgcagt ttcaagatat cacccgccaa aagttggaac atgtctacca
    26341 accattggag cgaattcatg cgccgcttca agcggtggtc aacgatactg aaggaataga
    26401 catgatgccc caagtgcttg aagaaatccg gaatctcgaa cacacctata cgatggagtc
    26461 tgaacgcctc accatggagg ctgccagatc ggggcaaggg acggtgatgg tcggaaccgg
    26521 ttctgatgga ggggacaaca tcacattatt ctgaagaagt taggtgtgga gggtcaggcg
    26581 taaaaaggtg ggtatttttc ttccgtccca ctcctgacgc catacgaaaa attgggtaga
    26641 agccgaacga acagtggtga tcgttcaggg ttaataatga aatacacaga accagagtga
    26701 gggaatgcta tgggcaagac tgttttagtt gtggacgatt ccgtctcaat gcgtcaaatg
    26761 gtgagcttta cgctgaccgg tgccggctac gaagtggttg aggctggaga cgggaaagaa
    26821 gccgtggata aactcaatgg tggggcaaag ccgaatttgg tgattacgga tctgaatatg
    26881 ccgaacatgg atggaatttc tttgataaag gcagttagag ggatgcccgc ccataaattc
    26941 accccgattc tgatgttaac caccgaatca tctgaggaca aaaagaagga aggccaaagt
    27001 gcaggagcaa ctgggtgggt cgtgaagccg tttaacccgg aacaaatgtt agccacgatt
    27061 aaaaaagtac tgccggggta ggagacatga agcctaaggc gggaaacgta aagcgaaagt
    27121 tgatgtgaaa tttgaaaaat tcacacaaaa cattgaatct atggaatgtg gcacagccac
    27181 ttgtggctaa tgagtacaga agatcaaaaa attatcctga ccaaaagaga ttttggccta
    27241 ccaatcaaat acggggaact gtagtccgta tttccagtca tatcgcggaa ggtgcggcgg
    27301 ggcagacatc aacagaatgc attcagtatt ttcacgttta tcagaggttg ggaaccaaac
    27361 cggatactca actgggaatt gccaagcgac ttgggtggtt acagaaaaag aatggggatg
    27421 agcttgagaa tgcaatgagc cgaatggata agatgccgag tggtttaatc cggcatcaac
    27481 aatcgtcccg cccttcacgc gttatccttc acgaaagaca aatcctcagt acattcgcaa
    27541 aggagtcgaa taatggcggt tgatctctca cgttttcaag agtcgttttt tactgaatcc
    27601 gcagaacatg tggaaaccat agaatccggt ttgttggcgt tagagcaacg tccgaaagat
    27661 ctggacttac tgaatcgaat ttttcggggt gcccattcga ttaaaggtaa cgcggggatg
    27721 tttcatttta ccgccattgc cgggttgact cacaaaatgg aaaacattct ggatgatctc
    27781 cggaatgaga aaatgccggt gacccctcat gccatcgatg tgctgttgcg ggggttggac
    27841 ggcttgaaaa gtctgttgga tgcggcgcaa gggggggagt cggcagatga gacagccatt
    27901 caggtcgtag aagctgaact ggaagcctgt cagaagggat cgactgccca tgcacatgag
    27961 gagtcttcct caaaaactgc tctcaagagt tctccccctc aaacggcgac ttggcgaatg
    28021 atccaaattg attggagtcc cttacctgaa ttatttcagc gaggaatgga tccggctcag
    28081 attttcaaag aacttcatga gttgggcctg gtgaaagtgc tgcaagtaag aactgatggt
    28141 cttcctccct tggaaaccct cgatccagaa cgttgttatc tcagttggac cgtggaattg
    28201 gaaaccgatg catctatcgg tcaggtcgaa gaggtgttcg catttgtacg ggacgggagt
    28261 gaattgacca tcattgatcg tgctccgaaa caggcagaac gctataaacg ggtgggggat
    28321 attctggtag aagaaggggt ggtgaccccg gagcaagtaa ccgaaagtct ggccaagcaa
    28381 aagcctcttg gcgaaattct ggtggaagag aaaaaggcgt ctcctcaaca agtcgataag
    28441 gcccttcaaa agcagcagca attgaaaaag aatgaagtgg catccattcg ggtcgatacg
    28501 gaaaaaatcg acaagttgat caatttggtc ggagaattag tgattactca atcgatgatt
    28561 acggatctca gcgagaagtt ctccatcgat cagctccccg ttctccagga acgtattacc
    28621 caattggaac gcaatacccg agagattcaa gaacgggtca tgtccattcg gatgatgccg
    28681 atcggcagtg cgttccaccg gttcccacgg ttagttcggg atctcgcagg caaaagtggt
    28741 aagcagatcc aattagtcat gtccggcgag gaaacggaat tggataaaac gctgattgag
    28801 gcgattggcg atccgctcac ccatctggtt cgcaattcgg ccgatcatgg attggagcta
    28861 cccgaagacc ggttgtcaca aggcaagtcg gcccgtggaa cgattcgact ccatgcctat
    28921 catgatggcg ggaatatttg tgtcgccgtg gaggatgacg ggcgaggatt gaatcgggaa
    28981 aaaatcatta aaaaagcggt tgaaaaaggg atcattgccg atggaagcaa tatgtcggat
    29041 gaggcggtgt atcaactgat ttttcgtccg ggtttttcga ccgccgagag tattaccgat
    29101 gtgtcaggtc gaggggtagg catggatgtg gtgaagcgga atatcgaagg gcttggtggg
    29161 tcggtggaca ttcagagcac gatgggcaag ggttctcgtc ttacgttgaa attgccgctc
    29221 acgctggcca ttattgatgg gatgactgtc cgggtcggtc aggataacta catcataccg
    29281 cttattgcgg tcaccgagtc cattcggcct aaacccaatg aattgcaacg gattgtgggg
    29341 aagggtgagg tggtcagtct tcgaggagaa tgggtgcctg tggtcaaact ttatgaggct
    29401 tttaacctca cgccggattt tactgatcct tcacaagcct tgttggtgat cgtggaagcc
    29461 gatggtcggc gattggccgt gttggtggat gaactgacag gacagcaaca agtagttatt
    29521 aagagtttgg agcaaaattt ccgaaaggtt gaggctattt ccggagccac aattcttggg
    29581 gacgggcaag tagccttgat cttggatgtg ccggggttag ccaaattggc aagggttggg
    29641 cacatcgcgg cggcataaga gaccgtgagg ggaaaaggac aaagggcact gaagttgtga
    29701 agtttgttat gcgccttata cttcacacgt ttcaagacaa aataatatat gtgttgagcc
    29761 aaaaccatga cgtacaaaac aaaggagtct aaccatgacc accgcaacgg ctgaaccgat
    29821 ggagcaacgc tcgattcgaa cgacgtatgc cagcgacgat caacaatatc tgacctttaa
    29881 tctggctgat gaatattacg gagtagatat tctgaaagta caggagatca agggatacac
    29941 cacggtaacc aggatcccca ataccccgga tttcctcaaa ggggtgctca accttcgtgg
    30001 caccattgta cccattgtgg atcttcggat gaaattcggc atgggggtga ccgaacccac
    30061 ttcgtttacc gtggttgttg tcgtcaatgt ccggaatcgt gtgatggggt ttctggttga
    30121 tgcggtttcc gatgtgttgg atatgaatgc caaggatatc caggcccctc cggacatggg
    30181 tacctcagtc gatgtcacgt ttatcgccgg cattggtagt gcccaggatc gcttagtgac
    30241 tctgttggac attgatcggg ttctcacgga ggaggaagtc accgtagtgg ggaagattca
    30301 agatgtggaa aaaaaagctg aagtccatgc ctgacccgaa tcgacatagg ccatggatgg
    30361 tccaggaggc cagaggcctt taacaatttc aaggaaatca gcgaggcttt tatgaacagg
    30421 atggtgaaca aaattttccg tttgggaatc gcaaccaagt tggtggtgtt gttcgccatc
    30481 tttggcatag tgccgatggc cgcggtgggg tatttagggg cttccgctac gggggaaatg
    30541 gaaacagccg ccggactgcg cgtgcaagcg atggctgaga ccattggaga taaaatcgac
    30601 cgtaatttat tcgaacggta tggggatcct caatcctttg gtcttaatag gatcgtgggt
    30661 gagcgctacc attggtacag caaggagaat aatgcgatag cgagtgccat gaatcaatat
    30721 gtcgcgactt ccggaattta ttatttgacg atattggtag atcctgctgg agatgtcatg
    30781 gccgtgaact caaaggatgc caaagggaat tcgatcgaca ccacggcaat ttattcaaaa
    30841 aaatatacgg acgctccgtg gtttaaggcg gtggaagcga aagaatttac cacaagacaa
    30901 ccctttacga gccccggcaa tgatacggcg accgggacct acattgaaga cgttcatgtc
    30961 gatgaagatg tgaagtcggt gtatttggga gatgacgggt tgaccttggg tttttctgct
    31021 ccggtgtatg tggatggcga tttgatcggg tattggacca atcgggtcaa attttctttg
    31081 gtggaagaaa tcgtgcaatc cgcttatcaa gagcttaaag gggccggatg gatggggtcg
    31141 gaaatttctc tgctcggaga tcaggggcag gtcatcgtgg attttgatcc ctccaatcaa
    31201 ggcacagagg acatgaccca tgattttgaa aagacgttgt ttaaactcaa tctggcagac
    31261 aatggagtag aaacggcaaa attcgccgtt gctggaaaaa caggctatca attagccctc
    31321 catgatgaga agaacatctg gcaaatgggt ggctataccc atctgaaagg cgccttaggc
    31381 tatccgggga tgaattggtc tgtcctggtg agagttccta aagccgaagc cactgctgcg
    31441 gccattgcat ttaatcgaga tttaaccatt accgccatta tttgtctggt tctgattttg
    31501 gtcgttggat tgtgggttgg tcgtcgtggg gcaaataatc tggttcaaat tagcgaagtg
    31561 gctcaacggg cggccgatgg agatttgagt cggcgggtca ccgtgatgtc caatgatgaa
    31621 ttgggaacca tgggcaaggc gatgaatgtc atgttggata atttggtgag agtggtatcg
    31681 gaagtccgcc aggctgcgga acatgtctct atcgcatcag gtgaaatcac ccaaggtaat
    31741 gaagatctct cgcaacgcac ctcggctcag gcaggcgcct tggaggagac cagcgcgtcg
    31801 atggaagaga tgacgtccac catcaagcaa aatgccgata acgccaagca ggccaaccaa
    31861 ttagcggtgg ccgcccggga agtggcagag aaggggggcg cggtcacgga caaggctgtg
    31921 gtggccatgg atgagattaa taagagcagc aagaagatcg cggatattat taacgtgatt
    31981 gacgaaatcg cgtttcagac aaaccttttg gcactcaatg ctgccgtgga agctgcacgc
    32041 gcaggtgaac aaggccgggg ttttgctgtg gtggcctcgg aagtgcgaaa tctggcacaa
    32101 cgttcggcct cggctgccaa ggagatcaag acactgatca atgaatcggt gcaaaaagtt
    32161 ggtgacggca gcgagttagt gaatcgctcg ggccagacgc tgggcgagat tgtgaattcg
    32221 gttaaacgag tcacggatat tatttcggaa atcagtgcgg cctcccagga gcaggctgcg
    32281 gggatcgatc aggtgaacaa gtcggtgatg cagatggatc agggcacgca gcagaatgcg
    32341 gccttggtag aggaagctac gtcggcttcc caatccatga agcagcaagc gactgaactg
    32401 ttgaatcaag tcgcattttt taaattagaa gatacagaac aaaaacgggt tgcagggaca
    32461 caacccggat cgggagccaa ggttgcgctt tcaagtggca ataattcctc cgccatgagt
    32521 aagcctttac attcagtgaa gggtgtcccc cctccaaagg ccacggcgaa gactcaaccg
    32581 gttggcgtgg gcagttcgaa tggccatgac cgccgtcaac gtcaggatga tttttttgaa
    32641 gagttttaga agacgtgacg ggtcaatcgt gaggggagag acgaaatgac agaaaagtgg
    32701 gaggccaatg gcgttgcctt ttcaggcatc ccggccggtt ggtagggggg atccatccta
    32761 cgccttacgg ttcacccatt acgcgggaca ttgaggcagg caagtttgtt tatttatcaa
    32821 ataagatagg aggtcgcatg ggtaaagtat ggaactcaat aggcatcggt ccaaaggtgg
    32881 tgatgactat tatggtggtc ttttcaagtt tacttgcagg gggagcgtat ttgctcggac
    32941 agcaattgca ttccgatctt gaacgggtct tgtccgatca ggcgtttatt gttcaaaaac
    33001 aaattgaggt cactcgtgcc tatgtgacca aagagtttgt ggtgaaggcc aaagcggctg
    33061 ggatgaacat gggtgttgat catagtgctc ccgatacggt tccctttccc gccacatttg
    33121 cccgtgaaac ctctgaacta ttggctgagg aaggggtctt taatgcccgg atcgtgagtc
    33181 tctctcccct gaatccgaaa aatgctccgc aagatgcctt tgagaaagag gctctagctg
    33241 ccctatctgc gggccaaaag gaatttacca aaaacgatac cgtgaatggg aaaatgatgt
    33301 tccggcgtgc gaccccggat ttagcgacga cccaggcctg tataggctgt catgagggta
    33361 aaaaaattgg ggagatgtta ggagcggtgt cggtacaaat tcccatggat ggtcccgcgg
    33421 cacaattaca agggaatctc agcaatatgt atatggggat actggccgta ggtgtgttga
    33481 tgatggggct gttgtatctg atgattgcga agctggtggt caagccgatg aagagtctgg
    33541 agtccatggc ggagcgagcc aaggaaggag atctcacagg tcgggccagc gtgaacagtc
    33601 aggatgaaat cggtcgaacc agtgaatcgt ttaatatgat gtttgaccgg gtgagtgaag
    33661 taatcgggtc tgtaaaaacc gctgtttccg gaattacgac agggacttcc gagattaatg
    33721 caggaacctc cgatctcgcc gggcgaacct cggcgcaagc aggagcccta gaagaaacca
    33781 gtgcgtccat ggaagagatg acctctacca ttaaacagaa tgcggataac gccaaacagg
    33841 cgaaccaatt ggcggtggcc gctcgggaag tggcggagaa gggtggcgcg gtcacggaca
    33901 aggccgtaga ggccatggat gagattaata agagcagcaa aaaaattgcc gatattatta
    33961 atgtgataga tgaaatcgcg tttcaaacaa atctcttagc tttgaatgcc gcggtggaag
    34021 cagcaagagc gggcgagcag ggtcggggtt ttgcggtggt ggcctcggaa gtgcgaaatc
    34081 tggcacaacg ctcagccacg gcggccaagg agattaagac gctgatcaac gaatcggtgc
    34141 aaaaagttgg cggtggcagt gagttggtca atcgctcggg tcagacgctg gccgagattg
    34201 tgaactcggt caaacgggtc acggacatta tttcagaaat cagtgccgct tcgcaggaac
    34261 aggcggcggg gattgatcaa gtgaataagg cggtgatgca gatggatcag ggcacgcagc
    34321 aaaatgcagc cttggtggaa caggcgactt cagcatccca gtcaatggcc caacaggctg
    34381 aggatctgtt gcgtcaggtc gagtttttca aagtccagac tgaattcgcg gggcaacgtt
    34441 ctgagcctgt ggcgaaaacc ggctcatctg ttcacactca atcaaccagc ggaacggggt
    34501 ctcaatattc tcgttcggcc tcgtctatga atggtaaagc tcagggaaag cattccccaa
    34561 ctaaaaaaga ggtcgctgtg ggtgttggaa gttcggggac caacgggcat aaaaaggccg
    34621 gggatgattt tttcgaagaa ttttagacac cagtaagtcg taaatcatta atggtaagga
    34681 gcgtggggaa aatccatttg ggattttctt ccgctcctta ctcctgcgtt ttctccttcc
    34741 gtaaatttaa ttgactgttc ctggttatgt cggataatct acagtcccga accagggcat
    34801 ctaatccgtt catatcatat tttcaacact cacaggagga gggttgttat gagaagatgg
    34861 gctgcattcg caatgatgtt tgtcgtcttc atgattgggt tcgccctggt tgtaccggtg
    34921 ggccaagccg cagacgcggc taaggatttg gcggcgaagt acatcctggc caccgctaaa
    34981 gctgccagaa cggtgtacgt caaaggtgtg gtggccgatg cgggcaaagg tggcatgaaa
    35041 cctgatgaaa attgggtgaa ggatgaccac gctatgatgc tcccagctca attcgtgaaa
    35101 gagctcggaa aggaattaaa agaatttgat ttgtccttgg tgggaactga gcccttgtat
    35161 gcttccaatg cacccaagag cgcaaaagaa aaagaaatgt tagcagaatt agccaagggg
    35221 aaagaaaaag tgatcgtggg agaagatggt ggagtgaccg tcggtatgtc ggccgattat
    35281 gctatcgctg atagttgtgc tgattgccat aacaagcacc ctaaaactac caaaaaagat
    35341 tggaaaaagg gtgattttat gggagccatt gttgtcagac tgaaataatt tcgttgtaag
    35401 actaatcgtc ttgaggaaag gcggggaagg tatcactacc ttcctcgcct ttttttattc
    35461 ccaccccggg cttccatttt tttaattacc tccggtcctt acattcggtc ataggtgtgc
    35521 cgataacatg aaatggaaaa ggaaaaagaa gtgaggctga agggtgagtg gtaaaaccaa
    35581 taggccaacg tttcatgcca catgaaaaaa tttgattctt gtatccttcc aattttcttc
    35641 actttattga ttccctggag gggaaaattt tcaacagaga atatggttaa aaaaagtaat
    35701 ttttcgaatt gggctctggt cggtaagacc accgcccaca atcttgctct ttttgggtgc
    35761 aggagaaact gctgatgatt ttcctcggta tggtagctct tgagtcaaaa gtcaccaatt
    35821 aattgaatgt cggacatgaa tagttgtacg gaacagtctg gcaatgttgg gcccaagaat
    35881 atgccgaatc ctcgtgtgca tacggactcc gcggggatca cgatagggtt gggaatcacc
    35941 aatacagtac ttgaactccg gaagcttgga aatggagtgg agtttggtgg atgtggggag
    36001 ggtcacttgg catcgtcttc ctcaggcata tgatgcaatt gaaatttctg gaaaaccttt
    36061 tagcgttctt tatagtccat ggaatccgat ctttatgggt tccaggatgg tgagaagcaa
    36121 ggcgtaaggg aggtgtgagg tgttctttga ctccttactt ttcactcctc actatcaatc
    36181 ccctccctgt ttggtttgga tcaattgagg ataaaggaca atatgatgac gacaacgttg
    36241 agtgataaag aatttgaaca gtttaaacac ttaatttatc agcaggtcgg gattaccctc
    36301 gatgtcccta aaaaaacgct cttagtttct cgtttgggaa aacggcttcg ggatcttcag
    36361 ctttcctcct atcaggccta ttacgactgc gtgagcggga aaggtggaga agaagagttg
    36421 atgaagttgt tggatcttgt gtcaacgaat aaaactgatt tttatcggga gcctgtgcat
    36481 tttgattttc tccgcgacca ggttcttcca gagatgcaat cggccaagac tttgagaatt
    36541 tggtcttccg cgtcttcttc cggggaagaa ccctatacga ttgccatgac cttgtccgat
    36601 gccattgccg atatcaatcg atgggacatc aaaattctcg cctcggatat ttctacgcgg
    36661 gtgctggcaa aggcctcctc gggtatctat gaggaggagc gggtgagtca acttcccaat
    36721 gatctggtga agcggcattt tctccgtggg aaaggtccgc aagaggggaa actacaggtg
    36781 cgcccgcaga tcgcgcgcct tgtgaccttt cgacggataa atttgatgga tccgacattt
    36841 cctatacgca gtcaactgga tgtgattttt tgtcggaatg tgatgattta tttcgatcgt
    36901 cccacacaag ccaaactcat ggataagttt ttccgatatc tccgccctgg tggatatttg
    36961 tttatcgggc attccgaaag tctgcagtgg atcgatcatc aatttacgta tctgcgccca
    37021 accatttatc aaaaaccagt taacgtgaag agtgaggcgt aaggggtgaa gggtaagtag
    37081 ggaggagtgg cgaaatggaa atattcatcc cgtttctcat gcttctgact ggtgtctctg
    37141 tactttaagg aaattatgtc atctacagga ttgatggaat ttggacatat tcggcggatg
    37201 caggataccc gcttccccta cgaagtggcc gtcattcttc caggtgaata ttttgtaagc
    37261 cgggaaccca aagttgttta tacggtatta ggatcctgta tctcggtgtg tctgcgggat
    37321 ccagaagccg gcgtgggagg gatgaatcat tttatgttag ccgcaccctc caacactgaa
    37381 ggacacgata attgggcgga ttcaggacga tacgggagtt ttgccatgga aatgctgatg
    37441 aacgatattt ttaaacgtgg cgggaaaaaa gagcggcttg aagcgaaagt ttttggcggg
    37501 gggaaaattt atgatggcac cattgatatt ggggctcaaa atgccgcttg ggctctcgcc
    37561 tttttggaac aggaaggtct ttcgcctatt aaggcggatg tcggagatgt ctgtcctcgg
    37621 aaagtgtact atttcaccga ttcagggaag gtactcatga aaaagctcga ccgtgtcgtg
    37681 gcgagtgcaa ttgcgcaaga ggaggggcag tatcgaaaga aacttcaaca tgctcctgtg
    37741 cagtccgatg tcacactttt ttgagaaaac acgacaagta catggagtaa ggcgtgagga
    37801 gagaggaatg gccagagaac agaccatctt ctttttactg gggactcctc actgtgtact
    37861 cttttctgaa ttagggggtt taatatgaat gggaaaaaaa tccgtgtgtt aattgttgat
    37921 gactcggcgt tgattcggaa tgttatgacg gagattctat cgcaagatcc tgaaatcgag
    37981 gtggtcggaa ccgctcctga cccgtatgtc gccagggata agatgaaagc ccttcatcct
    38041 gatgtcctga ccctggatgt ggaaatgcct aaaatggatg gactcacctt tttgcagaaa
    38101 atcatggcgg
//
LOCUS       DAORAQ010000011        36591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_248_length_36591_cov_62.911402, whole genome shotgun sequence.
ACCESSION   DAORAQ010000011 DAORAQ010000000
VERSION     DAORAQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 36591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36591
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_248_length_36591_cov_62.911402"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="trpB"
                     /locus_tag="PKM72_02130"
     CDS             <1..1158
                     /gene="trpB"
                     /locus_tag="PKM72_02130"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNP59605.1"
                     /translation="QFGGKYAPEILMPAIHELEQAYHAAKASQPFQQELRDCLKQYVG
                     RPTSLFFAERLTKTLGGAKIYLKREDLCHTGAHKINNTVGQALLTRRMKKKRIIAETG
                     AGQHGVAVATVAAMFGLEAEIYMGTDDMERQALNVFRMRLLGSKVTGVNSGSRTLKDA
                     ISEAMRDWTTNVSTTHYLLGSVLGPHPYPMMVRDFQSVIGRETRKQILALEGRLPHCL
                     VACVGGGSNSIGLFYPFVKDTQVKMVGVEAGGYGIKSGKHAARFAGGKPGVLHGTMTY
                     LLQDADGQVNPTHSVSAGLDYPAVGPEHSYHHDTGRIRYTSATDVEALAAFDLLAKEE
                     GIIPALESAHAVAEVVKLAPTMKKSHIIIMNLSGRGDKDVQQLAKMRGIPL"
     gene            1222..2079
                     /gene="trpA"
                     /locus_tag="PKM72_02135"
     CDS             1222..2079
                     /gene="trpA"
                     /locus_tag="PKM72_02135"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNP59606.1"
                     /translation="MKSFGMSNRIQDTFAQLHSKGEKALIPYIMAGDPNLAMTESLVL
                     TLEQGGADLIELGVPFSDPIADGPVIQQAAERALHSGTTLKKILATVTNLRKKTSIPL
                     ILMAYYNSLMAMGITDFCANAIQAGVDGLIVPDLPPEESDILYHAAQASGGPVNIFLL
                     APTSTAERRRAVIQRSHGFIYYVSLTGITGAKLTDLSRVRQSVHQLQRTSRKPVAVGF
                     GIASAEQAREVGRFADGVIIGSALVRHIGEHHSATTFLTDIQGMAQEWKSVLLPVTGK
                     SSTRQKPKT"
     gene            2271..3197
                     /locus_tag="PKM72_02140"
     CDS             2271..3197
                     /locus_tag="PKM72_02140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNP59607.1"
                     /translation="MAPARKTPRKHSTPSSSKTGSSSREQLATAPDSSLFRLVNHRLE
                     GNKVNYLETPNKGGTLQPSYLVFHYTAGRSSQSSIAWLTNPLAKASAHLVVGRDGTIT
                     QLAPFITKTWHAGVSHWDGLTGLNQHSIGIEMDNAGPLIAVGTKLTAWFGKSYPKSQA
                     MFATHKLDTEPKWWHAFSEKQILIAVELAKILVSQYALKDILGHDDIAPDRKRDPGPA
                     FPLDHIRALVIGRSQEEGDWYQVMASALNIRSGPGSEYDIVASPLTKGTKLLALEKQD
                     RWTKVDVDPSALSSGQDLEGWVFNRYIESLDA"
     gene            3450..3650
                     /locus_tag="PKM72_02145"
     CDS             3450..3650
                     /locus_tag="PKM72_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59608.1"
                     /translation="MGETEYLDQEDQEKAVEIRIEDVIYRSDESPAVVLAIKRTCSLS
                     GECKVGHVTLGHENTLRLYLET"
     gene            complement(3866..4321)
                     /locus_tag="PKM72_02150"
     CDS             complement(3866..4321)
                     /locus_tag="PKM72_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNP59609.1"
                     /translation="MIGIISDTHGLVRPQVIEVLTGAELIIHAGDIGNPDVLKMLETI
                     APVIAVRGNNDHDPWAEEIPLTNVVEHQSYFFYVLHELDDLALDPVASEISAVIFGHS
                     HRASAERRKRVLYLNPGSAGPRRFTLPITVARLHLTDTGLTPEIIEVEN"
     gene            complement(4357..4887)
                     /locus_tag="PKM72_02155"
     CDS             complement(4357..4887)
                     /locus_tag="PKM72_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59610.1"
                     /translation="MTRSKLFPGYLIALALVILLGDYVAGPTIQFPILYLIPVSLASW
                     YSGFFWGLFFAVTMSLIRLYFYGMLWPIHLAMFDASINTIIRILVLGGMAFLVNRTAE
                     QTRALAQRVNVLEGFLPICSFCKKIRDSNNIWQPLEQFITDRSNAQFTHGFCPECGQR
                     HYGDILKMGDQPTKES"
     gene            5427..6995
                     /gene="zwf"
                     /locus_tag="PKM72_02160"
     CDS             5427..6995
                     /gene="zwf"
                     /locus_tag="PKM72_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNP59611.1"
                     /translation="MKPSHPVFDQAEDLKESPLFQQRGNGQTRPPTLFVIFGAQGDLT
                     KRKLIPALYNLASSHHLPQEFAILGIDGIPMHTNDFREKIRQDIDELCPRPIDATIKE
                     WLLDRLHYLSGDFRDPQTYERLQVLLTQLNTTHGTQGNYLYYMATAPTFFSEIVQQLG
                     AKGLVEYRKEFSRRIVIEKPFGHDLPSARSLNQALKQVLDESQIYRIDHYLGKETVQN
                     ILIFRFANGIFEPIWNRQYIDHVQITVAETLGVEHRGAYYEKAGALRDMVSNHLLQIL
                     AFVAMEPPNTFDPEAVRNEKAKVLRAIQPMNPVEVLQSTVAGQYEAGSINGHSVLPYR
                     TEKDVDPASLTETFAAMTLGIESWRWEGVPFYLRTGKRLHTRVTEVVIQFKSAPLMLF
                     RKTPVDRLTPNVLVIRIQPDEGISLSFGAKIPGPKVQVGTVDMDFQYAEYFGDAPSTG
                     YETLLHDVMAGDATLFQRSDSVEVGWSIVDPIVKVWESLGSHAIRPYSSGSWGPPEAD
                     ALLAKDGRMWRNHG"
     gene            complement(7042..8475)
                     /locus_tag="PKM72_02165"
     CDS             complement(7042..8475)
                     /locus_tag="PKM72_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNP59612.1"
                     /translation="MRNHQPASDISWTREAILYQIFPDRFARDSHETKSGPGIVPWRS
                     KPTAHGFMGGTLRGITERLDYLLDLGVNLIYLAPIFFASANHRYNTYDYFRIDPRLGS
                     LADFQGLLSKAHANDIRVLLDGVFNHCGRGFFPFYDVMERGQASPFASWFYITRFPVN
                     AFGPFNYRGWQERPLMPILNLANPATRRYFLEVAAYWTNQGIDGWRLDAVADVRGHRF
                     WKALWHEVKTINPSAYMLAEIWGPGRSWIRDGQFDGGTNYVFRQIVLDYFIQGSISTR
                     KFVSRVSKLLRMYPWKKTSAMVNLLGSHDTERLYTVAQGNIERLKLALLFQFVFPGIP
                     AVYYGDEIGMEGEKDPDNRRGMEWNQSKWNDELRDFTKRLVAIRKSLPTLRQGDWTVI
                     QELPDQQCCVFLRKTKRSVVFILIHNDDHPVSIQIDLAPWVGKTARWLEDHLTGNKFK
                     CEKGKVVLADFGPRSGMILTPVSQAGA"
     gene            complement(8614..9159)
                     /locus_tag="PKM72_02170"
     CDS             complement(8614..9159)
                     /locus_tag="PKM72_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59613.1"
                     /translation="MAATPKNSSKGKNVGAANKAKVEFQEYKKRIEHAPAAEHGGPQF
                     GTGATGMPQMPMGWPFPPMTGFMPQIPQMPHLNPALGQTVSETSGEIADRVGETLKLG
                     MDLLNSGLGVGLRFLQGLAGPEYHHGDAGWGHAHGAGDYSHGSHGYGCQMTCGCDCAD
                     CCQHSGCASSCCCSPSVGSCC"
     gene            complement(9183..9758)
                     /locus_tag="PKM72_02175"
     CDS             complement(9183..9758)
                     /locus_tag="PKM72_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59614.1"
                     /translation="MSGPLFQHGGAGFCPGCCHPRAKCCCGHRECRKEAKELLVEADT
                     NVDGKTDSHSMVLQRVRAMAMFAAPVELSVDDTVGKNAETKAAVAPIVGEVDVRGKAG
                     IGKAFIGGGCCVHLSIEYTPTNAASTAPALVMVAVQDSEKTTLGWVKQAEPQDGYVIK
                     EGIITTKPGAHLTVLVVNMTARVRWCEVFSC"
     gene            complement(9911..10387)
                     /locus_tag="PKM72_02180"
     CDS             complement(9911..10387)
                     /locus_tag="PKM72_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59615.1"
                     /translation="MHHHSPHCCVPTSHHSSPHCHSTGYRSMAPMGYPVLMPMPYYPS
                     VGYAGYGWGGQATHSMPLPQEVSVDSATGMKEVLIGGTTKASLTLEYVPDGGATSPLV
                     KVTVEIDGSISTWQETSMTDGYHVKSQLLSIEPGAKVTLEVKEAMARLRWCETICC"
     gene            complement(10387..10581)
                     /locus_tag="PKM72_02185"
     CDS             complement(10387..10581)
                     /locus_tag="PKM72_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59616.1"
                     /translation="MATVIGSVNKLINQTSTQILENHIPQKLADMINTVVNTGFQVVE
                     DVLKITQDLTKASDSNEGDS"
     gene            complement(10625..12085)
                     /locus_tag="PKM72_02190"
     CDS             complement(10625..12085)
                     /locus_tag="PKM72_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNP59617.1"
                     /translation="MTYDVIVVGAGPGGSSTAAFTARKGLSTLILDRTDFPRDKVCGD
                     GLTPQALYWLDQLGCIDEVLTHTKSCIKTCDLFINGELLLTGGFPNSTQYPDFCVLLD
                     RRRFDHILLQHAVDSGAKFQAHSLVREIHQKPDHARVVLDHKGQRTEVQGKIVIGADG
                     VSSVVSRSIGNVLKNGATALSLRAYYQNVDAEGAQIKVYFDERFFPGYAWVFVDDDGF
                     ANIGLGYAFDKPFPMIPNLKQVFQDFLDNDLGHLLRHASRCGAVSGGAVSFFKPKAIV
                     ADRVMLVGDAANQADPLNGGGIHKAMEGAYLAAETAHQAVLQGDCSVRVLQQYQMQWE
                     QQFDLDWRTGELFLSVAKNPDLKEFCLFVLANIARMTTHDRQFQEFCSGVFSGVLSQN
                     ICLVPQALFHAMPRNPAAWLAMLQLDEKGVVTGPLSLATDALTSSAKASSRMLMNPWQ
                     SLNWSLEVMTKVLRLARGHAEGSLTPTGTALTKCPI"
     gene            complement(12145..13134)
                     /locus_tag="PKM72_02195"
     CDS             complement(12145..13134)
                     /locus_tag="PKM72_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59618.1"
                     /translation="MGRPYWDRPLGRLLASPSIWSALVPNFSGNSCTGLFGAIHLVPT
                     DAHVPCVRDVDPLSSTIIVCNANLHPVRAIHVVNPHNVFGVMSMKSGSKATAGKNSKK
                     NPQPRRSPAQKTSTAQASAHTDQRINSEDSVFGHGDQIGTTFAKGLDLAEAGLSLGLT
                     LINRFGTIMQDSMMEKFSGVMGTATASPQESAPPTSSPRDPAAPMSSSEQSEGIEPQD
                     PAYYIANRLPLTPGQSVQVSFSINNDSMDSAKNVKLKVDGFIGSTQGQVFPCKGFTVN
                     PGTKTINPMDFEKFVLKGVIPDGLPPDIYHGRILVVSEQAIEIPVKLLVTSAT"
     gene            complement(13184..13951)
                     /locus_tag="PKM72_02200"
     CDS             complement(13184..13951)
                     /locus_tag="PKM72_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59619.1"
                     /translation="MGDIKIKEAKNLDKLRISELSNVDPLVISEVRNIAPAAVHIKEL
                     NHIDPLTIESLRIDEIRNLDPIRVEQFNVTNLPTVNLSLRQLPAVDMNIRRLPPVSIG
                     LHQDFCLPSSYTLRARVLGIELLRLNLDGRTMVSPRDRVRREQSRTPAQSFPQVATAG
                     NPAIPSRSMTKGSRVVATGLPCQPIPPSHQPMHGTFTERPIKGKDGLKPGGKFGGGRQ
                     APSGGLKVGNVPSHTFPMTKPRSQRDFMDSAVSSGEN"
     gene            complement(13976..14689)
                     /locus_tag="PKM72_02205"
     CDS             complement(13976..14689)
                     /locus_tag="PKM72_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59620.1"
                     /translation="MPVHTAPCRHSHPTYQVYHQIVCQHQIEVEVWPVNAEVYFRDER
                     YGDPVNSQVRFQASVYNGMSSKVNWAVQDPAGNPGSGSIDPTGLYVAPPKGSLAHGTT
                     DIIIATAAESPFRKAYAFVTLMGNGPEPLPQPTLEVFPKQAYLYYPQGHDNAYIDQSN
                     TQQLFRATTRHSSGATVQWLVDSVVQGGAGTAPWFLYQLNGSGSSKVVTISARLQTQP
                     AIIDEAKAIHLNYSWPGLV"
     gene            complement(14726..15154)
                     /locus_tag="PKM72_02210"
     CDS             complement(14726..15154)
                     /locus_tag="PKM72_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59621.1"
                     /translation="MNFKGQLGMESCPDFSVQAKTEPIAFATKAKGSFVAQVSSISVQ
                     VSEIPINLSIPFLRRAGGIHTVASIGSFGIKLSPFTVAVEGANVQLAGVLGTKGITTQ
                     LEGKVGCKSKLSFNGKLFGKIANCSVDLPDDDFEEHVDTM"
     gene            complement(15151..15357)
                     /locus_tag="PKM72_02215"
     CDS             complement(15151..15357)
                     /locus_tag="PKM72_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59622.1"
                     /translation="MAANMVLRIKLGPFVSFEIEGENCEEICQSLKGFEQLNERIDAM
                     CSDLAERVYPDGMTTQQTAQEEST"
     gene            complement(15458..16165)
                     /locus_tag="PKM72_02220"
     CDS             complement(15458..16165)
                     /locus_tag="PKM72_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59623.1"
                     /translation="MGRSPEDASGTTEVYWGDVFAIALQQLRSQGKVSDARPLLRIDG
                     QAEAAMAPYQFDPVYGKLHRRECSNIADDSRSALFARWSMGIDEQVLACSHCRPHPYP
                     RGQEGQDVTLDILFGVISILDQFGTVLRERGKEYRTSSDGQELERKLEDLYKNLDRQQ
                     KDTLEVMLHSMDQMVKFLREADRNLSQVNDKPGNGHVQTNGHGMAKKTANETGTMKAV
                     NGNSKRPVRRQDSRKSK"
     gene            complement(16282..17193)
                     /locus_tag="PKM72_02225"
     CDS             complement(16282..17193)
                     /locus_tag="PKM72_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59624.1"
                     /translation="MYASQSLHTPLDQLFSFYRKQVVVFLKPLVLEYYPDLFEQRQDE
                     YRKMMELSAKMTVVGHACAEIAGFPYDARRQMIGSLFGGCCFLADSFIDDFGLDVAHS
                     YIDRMDDLLTKGWFEIQTDREKLFYVIIARLFAERDVLDPFLRQAILLLFQAQKRDVA
                     FRSDPESIRSRPRREQLRVLRESARNRSGHAILVLTGFLVPAVPLHYLSMIFLAGSLI
                     MYIDDHGDCYSDLHYNRVTYLNQVADPERTLRRIFQGHIARLRQGLPEGSGRDLLVAF
                     LTKYFATRLKKHRLQKTHRELAWAVYD"
     gene            complement(17652..18803)
                     /locus_tag="PKM72_02230"
     CDS             complement(17652..18803)
                     /locus_tag="PKM72_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNP59625.1"
                     /translation="MPNSTWIDVGGVEDLKHQPVQQVLIGRTPVALTYRNGEFGAING
                     ACNHVGGPLGEGTLDGEYVVCPWHYWKFHYRTGKGEPGYEEDAVPAYEVKVAEGRVLI
                     RSTAISKRTRKAHEPHPLARTPHREKGPIRVLGLSTTVMTPDHPRYSTSEDLLEVGLK
                     HASENLDCETRLLRLRDLSFRPCEGFYSKSSRACTWPCSITQMDPQDEMDQVYEGLVH
                     WADVMVLATPIRWGAASSLYFKMVERMNCIQNQETISDTHLLHNKVGGFIITGGQDNI
                     QAVAGQLLGFFAEIGVQFPQFPYIAHSRGWSAEDMQNNMRYVQKSQSLREGATALVER
                     CVQMASVMIHGNLGECRTARGGRKAHELDTRAQLHGSTDAEKNPVAKPD"
     gene            complement(18886..19188)
                     /locus_tag="PKM72_02235"
     CDS             complement(18886..19188)
                     /locus_tag="PKM72_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNP59626.1"
                     /translation="MNEPIRMEHFPPYYYPHEAVVWAGVPIEWINATASPHTVQHDDC
                     GTGKPCLFDSGKVAPGKTYTLPGLPPGRYSYHCQIHPIMGGILIVEDSKGRDLPPQ"
     assembly_gap    19531..19630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19800..23954
                     /locus_tag="PKM72_02240"
     CDS             19800..23954
                     /locus_tag="PKM72_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNP59627.1"
                     /translation="MLRFPFKIFHRQLRRILVAFLSVGTFVIVDGSPAQAQTPPITSS
                     GLNTQVSAPRHLPGGAVQHDITGGTRPGGGSNLFHSFGDFGVPTDNIANFLNDSGLPT
                     SNILSRVTGGNPSNIFGTIQTKGFGNANLFLMNPAGIVFGPNAALNVGGATHFTTADY
                     VRLEDGAQFTALPGAQDSLLSIAPIAAFGFLGPNPAGISVEGSTLSISEGQPLSLIGG
                     DITIGRSNLTAPGGQIALASVGSPGEILANTFDQTSNIHGHTMGSGGTIRISDQSVID
                     AGGEHGGKMLIRGGRLVLDHESQIRANTLGAQDGGHVDIAVSENVLIAGTGAQTDTPS
                     GIFAKTEGGGKGGTITISASSVKLTDGGNLSVSTRGEGDAGTIMVNAPDGKVILSGSD
                     NRLTDFSPEEEAVSGLFANTVGTGRAGTITVQTGDLTISEGARISAGTFGRGNGDAGK
                     LFIAATGDVLLSGTQVNGITSSGLFAGASELTLPGNGLLGSGGTINMTGQTVRILDGA
                     KIHVASIGQEQPDMNRLDNAPSAGTVNIKAAMLDLTGAELDGKVLDIIPSEITASATG
                     PASAGHILLEADKITIKDGGGVTADTESITRIEKTGGIQLKAKENLVISGTAVRTEFP
                     PNLLPQNTLNPRTVVQSAVRAQTKGVNRGGEIHISAENLFVDGGGLISNAALNGAGAG
                     GNIHLDVVDTIRLSGAYEKVDKNRTIFRSEISSGTEGPGHGGDISITAKNVEMLDGAW
                     ISSRTRGRGSGHAGRVSITAEENVSLSGFMSVYDPEINNTSKTQEISSRIIARAEEHD
                     NRLADLPLGNAGDISITAKNVVMKDGAEIIAPSSSGRVIPITDAIEEIVANMPEEQRP
                     RAGNITVKADTIILSGARTVGGSGKFGWAPTTIRAGVIGPAGTGNVSLTANTIVIRDG
                     ADISAETQSSTQGVQRGNVTITAHDSLTLSGYLLRTEFPENPIGSDPFPEANGRTIRP
                     TSIVARTSGTNQAGDIILSAKNLIITEGAQIANGTLNHQLDNAGGGGKILLTADHLTI
                     SGMAPFPDAIGRPLPSIISSRTDGPGQAGQIDITSKTIEIKSGGQISASSIPREDAIG
                     AGAGGNIQVVATERFLAKGVGILEGTRGVRSGIFSSTEGTGLGGNLRITAPVIKMKNG
                     AAISASTTGTGNAGDIFITASDSILLDKASVTTEAGSAVGGNIKLTADNRIDVINSQV
                     TSRVREGSGSAGSINFDPDFIVLKNSQILSTAVAGSGGDITLTANSAIVMDPFTFLDA
                     RSRFGGNGRVNIESPVQFLSSTIVPLPQNLVPVTTLYGTKCVAEQGRFSSFVDSKADS
                     IAPTPGALLASPLLPFSSHSVASNPIDIHVDSNNQTQADPTHATTYSPPVLFSQATGQ
                     RATCRP"
     gene            complement(24226..24567)
                     /locus_tag="PKM72_02245"
     CDS             complement(24226..24567)
                     /locus_tag="PKM72_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59628.1"
                     /translation="MATWLIPALKAVLPHIGTIISATAPAFTKKDSAPTTDQTHLLQQ
                     QISELQTAASQNATHVKELAEQLQSTVAAIEHAASVATSNQQRALQISLAAIAISAIS
                     LCAVLLHVLFL"
     gene            complement(24748..26724)
                     /locus_tag="PKM72_02250"
     CDS             complement(24748..26724)
                     /locus_tag="PKM72_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNP59629.1"
                     /translation="MIEQFFLQSLLLLGTAVVVVVAFHRLHIPSPLGYLLVGLLLGSY
                     TPGPVIEAQPIRALAEFGIVFLLFTIGLNFSMPQIHALRHQLLGLGTGQVLLTTIAVG
                     LLAWLVGMPIAAAFVVGAVFAQSSTTIISKQLTDQGEEHSRHGRLGIAMSVFQDVTAV
                     PFVVVIPVLAMANTEVVVLASSLGMAFAKAALAFGLVFLAGRWVMRPLFYLVAELRST
                     EVFTLTVLFVSLVSAWTTSSLGLSMAFGAFLAGMMLGETEFRHQVESTIRPFRDVLLG
                     LFFIGIGMLVDPSTIPEIWHLALAGATVLLLIKTLLVALMVRIAGMDATTAWRTGWLL
                     AVGGEFGFALLSIALDVGVIQPKIGQIVLTSVLFSMIVAPFLIRYNQVLARWCAPRPA
                     REGKDAVPQVSIETVGHLRDHVIICGYGRIGQSVAHLLEEEQIPFVALDLDPSRVKEA
                     NIAGEGVFYGDASERTILEGIGVGTARLVVIGHADVASAHKTLHHLRTLRPDLPIMVR
                     TRDETHVEELRAAGATEVVPETLEAGLMIAAHALVLLDVPISRVARRMREQRKGRYHL
                     LREFFLGDSLLADTREEHNVDRLRPVVLPPDSSVVGHSLQELNLKGVAIAALVRQGQR
                     QLMPSGETRVEAGDVVVLFGSPDDLQRAERALFG"
     gene            complement(27116..27475)
                     /locus_tag="PKM72_02255"
     CDS             complement(27116..27475)
                     /locus_tag="PKM72_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNP59630.1"
                     /translation="MDVTSLAIIASGVFVGMGASFTGLGGGFLMVPLLLFLGFTAQKA
                     VGTSFVGILVIAVSALIAHGRLANVDYKYGLLLGLGGIIGAQIGARLIEGVPTDMFKK
                     IFSVILIGLAIYLFVKK"
     gene            27830..28687
                     /locus_tag="PKM72_02260"
     CDS             27830..28687
                     /locus_tag="PKM72_02260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP59631.1"
                     /translation="MSSAEFDSSQPISMAQLADPHLLYQKSVQSPELDMPFLTEYFES
                     YTNTPLRHFREDFCGTAFLSSHFVTRHPDNHAMGIDLDWPTLNWGIKHNVSHLTPEQQ
                     QRLTLVNDNVLNVQHPLSQLTVAMNFSYMVFRDRPTLLEYFRNARRSIQPGGLFMLDI
                     WGGSETQVEQEEHRDIDNPVDDGIGDFIFVWDQDVFEPSTYFCTTRIHFLFRDGSELR
                     NAFVYDWRLWTIPEVTELMKEAGFKDVHFLWEGLNAEANEGTGTYHRVEKGDSDPSWI
                     AYLVGQNPE"
     gene            28651..30243
                     /locus_tag="PKM72_02265"
     CDS             28651..30243
                     /locus_tag="PKM72_02265"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNP59632.1"
                     /translation="MDRLSGWPESRIRDGVSQTMALSLFECESIVGELNRALVGGFIQ
                     KIHQPRLLTLTLDVRTRGQTSTLLVSVEPRFARLHLTSQKFENPPTPPPFCSFLRSHV
                     EGGRIVEISQEPGDRIVFITIVKAEQVCVLVIALIGNQANVHLLNEKKLTLRSLRESR
                     VKVGERYAPPTLRPVKPPEALPPPSMAGRVPQNGIVIQQEGEMSEPLSESFTEGKGAF
                     REIFPVSAELEARYGQQEQEEGQDAILEQQLFQARKALKHAKRKIQALQEDFKKTERF
                     REYARYGELLKSHLHEIKKGQESIAMVDYYDPALPTLTLPLDPSKDAVWNMEDYFRKY
                     RKFIGAQEHLQPRVDHAQQEMARLEAKLAQLEQGIVDSDFSPKPSKTRGSSVPSGTRV
                     SKGKPAPAQAYRTYTSVDGLPILVGKTAKDNDHLTLKVANADDLWLHARGTPGSHVVV
                     RLEKGATVPPETLKDAATLTLWFSDLRKSGKGEVIYTLRKFVRKGKGFKPGSVTVERE
                     KSLWIELKEDRLKRLKGETSPG"
     gene            30821..31288
                     /locus_tag="PKM72_02270"
     CDS             30821..31288
                     /locus_tag="PKM72_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59633.1"
                     /translation="MRNICTLGLVGFFVLLGGCASNHTANDEQIDLYLNTLDDKHFVW
                     CELDLEQCRHDFEQWKDTAGGRRIIQEFEKEDTGQTDNIQHLPHVFRTRFVDEGQLGE
                     ELLGGEGKGQTFGQDTEGLVRSDSSIDENDHATQEGMSMIPKIQDPHFVPQIP"
     gene            complement(31564..32460)
                     /locus_tag="PKM72_02275"
     CDS             complement(31564..32460)
                     /locus_tag="PKM72_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNP59634.1"
                     /translation="MSTPDFNLLVTLDAVLAEGSVARAAQRLRLSPSAMSRALARLRE
                     TVGDPLLVRAGRSLVPTPRALELRERVRQLVQDGEAVLRPAEKLNLKQLVRTFTLRTG
                     EGFVENFGPALIARIGEEAPGVRLRFVQKPNKDSAPLRDGTVDLETGVVADTTAPEVR
                     AQALFRDRFIGVVRMGHALSKGKITPSRYAAGKHIVVSRRGLDKGSVDEALEPFGLER
                     KIVTIVGGFSTALALARASDLVATVPERHTGNLRAGMYSFPLPVSLPEVTVSLLWHPR
                     LDSDLAHRWLRGHVRDICAPIR"
     gene            32601..34091
                     /locus_tag="PKM72_02280"
     CDS             32601..34091
                     /locus_tag="PKM72_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP59635.1"
                     /translation="MLKPTIATQDVIVTGSAEQTPSIRWALIGLSLSMLLSSLDTSIA
                     NVALPTLTRAFTASFQEVQWVILAYLLAATTLIVSVGRLGDIIGRRRLLLAGIFLYTV
                     ASVLCATAPTFWLLIAGRAAQGLGGAIMMALSMAFVGETVPKAKTGSAMGLLGTMSAI
                     GTALGPSLGGVLIAGFGWRAIFLINLPLGILTFLLAHHYLPVDRLEPKTDRAGLDHVG
                     TLLLALTLAAYALAVTIGRGSFGALNIALLLAAAFGVSLFVLAERRAASPLIQLAWFR
                     DPVLSASLAMSALVTNVVTATLVVGPFYLSRALGLDAAMVGLVLSVGPLVAALTGVPA
                     GRMADRFGAQRVTIVGLIGMAAGSFILSMMSKTYGIPGYIVPIVLITGGYGLFQTANN
                     TAVMTDISPDQRGVTSGMLNLARNLGRITGASVMGAVFAYASATIDITTAHPEAVATG
                     MQITFGVAAMVIVVALALAIGTNRRTLHNREFAPEAEFQADGKVLE"
     gene            complement(34274..35065)
                     /locus_tag="PKM72_02285"
     CDS             complement(34274..35065)
                     /locus_tag="PKM72_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP59636.1"
                     /translation="MSLGRILALLSRHMYLYKRSFARLLEIFYWPFLDLVVWGFITIY
                     LEKVGMSLHGAVSFFLGALILWDVLFRAQQGIAVSFLEEMWARNLMNLFASPLTVGEY
                     LVATMTMSVLKVTAVGGLMMLFAWVFYSYDILQMGFSLLPFVLNLVISGWIIGVLTTS
                     IIMRFGQQAEVLAWGMVFLFQPISCVFYPIEVLPPVLQSIAWMVPPAHIFEGMRAVLT
                     TGDLPVMHLLWATGLNFGFLVIIVAWFYHTFSICKERGMLVRVGE"
     gene            complement(35062..35817)
                     /locus_tag="PKM72_02290"
     CDS             complement(35062..35817)
                     /locus_tag="PKM72_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP59637.1"
                     /translation="MPASGSNASPVVEVRNLTKRFQGFTAVDNISFDIKKGEILGLLG
                     PNGAGKTTTIHMLLGLITPTVGSIHMFGLDLATHRETILQQVNFSSTYISMPFSLTVE
                     ENLKVIARLYELKHIQQRIDDIVKKLEMEDIRHRLTRKLSSGQMSRLTLAKAIMTEPK
                     VLFLDEPTASLDPDIVNKIKSFLKEYQRSEGLSILYTSHNMREMEEMSDRIIFLQRGK
                     IVAEGTAPEIIQRYGQRDLEEVFLKLAREQNGP"
     gene            complement(35817..36515)
                     /locus_tag="PKM72_02295"
     CDS             complement(35817..36515)
                     /locus_tag="PKM72_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNP59638.1"
                     /translation="MGKGLTLIAATFLVLALGGAAAIPLTNQPTFCASCHTIKPSYDS
                     WIRSSHKDVTCVDCHVRPGISGFIEDKVFAGIEDVAITFFGTPTDPHNLKSHVTSSVC
                     INCHRAILRVTEVSVRDLPAPVKDVGLIMNHREHMEAFEKRGKGEGCTTCHGRVVHGS
                     PIKAYPIVIPRGHVKLDDQPHEPDHPKDSVLWKANMADCLRCHDGEQRYEGEVLSNRC
                     ETCHLPDKIGGFLF"
BASE COUNT         9518 a         9475 c         8973 g         8525 t
ORIGIN      
        1 caattcggag gcaagtatgc tccggaaatc ctgatgcccg ccatacatga attggaacag
       61 gcgtatcatg cggccaaagc aagccaaccc ttccaacaag agcttcggga ttgtctgaaa
      121 cagtatgtcg gtcgtccgac gtctctcttc ttcgccgaac gtctcaccaa aaccctcggt
      181 ggagccaaga tttacctcaa aagagaagac ctctgtcata ccggtgcgca caaaatcaac
      241 aataccgtag gacaagcctt gctcaccagg cgaatgaaaa agaaacgcat cattgccgaa
      301 accggtgccg ggcaacatgg agtagcggtc gccaccgtgg ccgccatgtt cggtctggag
      361 gccgaaattt acatgggcac cgatgatatg gaacgacagg cattaaatgt cttccgaatg
      421 cggttacttg gatcaaaagt gacgggtgtg aattccggaa gccgtacact caaggatgcc
      481 attagtgagg cgatgcgtga ctggacgacc aatgtgagca ctacccacta tttgcttgga
      541 tcggtcctgg gccctcatcc gtaccccatg atggtccggg attttcaatc cgtgattggt
      601 cgagaaaccc gcaagcaaat cctggcattg gaaggtcgtt tacctcactg tcttgtcgct
      661 tgtgttggcg gcggcagcaa tagcattgga ttattttatc cgttcgtcaa agatacgcag
      721 gttaaaatgg tcggcgtgga agccgggggc tacgggataa aaagcggaaa acatgccgcg
      781 cgctttgcag gagggaaacc tggcgttttg cacggaacga tgacctatct cctccaggat
      841 gcggatggtc aagtcaaccc cacacattcg gtgtctgcgg gattggacta tccggcagtc
      901 ggtcctgaac acagttacca tcatgataca ggacgaatcc ggtacacctc cgcgacagat
      961 gttgaagcct tagctgcttt cgacctactc gccaaggaag aaggcatcat tcccgcgctg
     1021 gaaagtgccc atgccgttgc agaagtcgtc aagctggcgc caacgatgaa aaagtcccac
     1081 atcataatca tgaatctctc cgggcgcgga gataaagacg ttcaacaatt agcaaaaatg
     1141 cgaggcattc ctctctagaa gatggcatga cgcctgccac agtgtataga gaaattcaag
     1201 agaggaaggc atcaggcatc tatgaaatct tttggtatga gcaatcgcat tcaagacaca
     1261 tttgcacagc tccactccaa gggtgaaaaa gccctcatcc cttacattat ggctggcgat
     1321 ccaaacttag ccatgaccga atctctggtg ttaactttag aacagggcgg tgccgacctg
     1381 attgaactgg gtgtgccatt ctcagatccc atcgccgatg gtccggtgat tcaacaagcg
     1441 gcagaacgag cccttcattc agggactacc cttaaaaaaa tcctcgccac tgtcacaaac
     1501 ctgagaaaga agacgtccat tccccttatt ttgatggcct attacaacag cctcatggcc
     1561 atggggatca cagatttttg tgccaatgcc attcaagcag gggtggatgg actcatcgtg
     1621 ccggatttgc caccggaaga atctgacatt ctttatcacg ccgcccaggc ttcgggagga
     1681 cctgtgaata tcttcctgct ggctcccacc agcacggcgg aacgacggcg tgctgtgata
     1741 caacgatcac atggatttat ttattatgtc tctctcaccg gtattacggg tgccaagtta
     1801 accgatctca gtcgcgtgcg ccaaagcgtc catcagcttc aacgaacctc acgaaaacct
     1861 gtggcagtgg gatttggaat tgcctcggct gagcaggcca gagaagttgg gcgatttgca
     1921 gatggggtca ttattgggag cgccctggtt cgacacattg gtgaacacca ttccgccacg
     1981 acatttctca ccgatatcca gggcatggca caagaatgga aatccgtcct cttgcccgtc
     2041 acaggaaaat cgtcgaccag acaaaagcca aagacgtaag gtgtccgcgc attcataaag
     2101 gctgccatcg ttgaatttgc actttcaatt cttattatcg atattcatgc aatattgttg
     2161 acagctctct ctgacgtaat tttctttgaa ttgaatcagc cttcctgtcc ctcgtgacgg
     2221 atccttccga cccaggaggc tcaaaaaatt cttaaaacag gaaagtgagc atggctcctg
     2281 ccagaaaaac ccctcgcaaa cattccactc caagctcatc aaagacgggg tcgtccagtc
     2341 gagagcaact ggccactgcc cctgattctt cactgtttcg tcttgtaaac caccggttag
     2401 agggaaacaa ggttaactac ctagagactc ccaataaggg tgggactctc caaccgtcct
     2461 acttggtctt tcattataca gccggacgaa gctcccaatc ctcgattgcc tggttgacaa
     2521 accccctagc aaaagcctca gcccatctgg tcgtaggccg ggatggaaca attacccagc
     2581 ttgcaccctt tatcacaaaa acctggcatg cgggggtgag tcattgggat ggcctaacag
     2641 gccttaatca gcattcgatt gggattgaaa tggataatgc cgggccatta atcgcagtgg
     2701 gaaccaaatt gacggcgtgg tttgggaaat catatccaaa atcccaggcc atgtttgcta
     2761 cccataaatt agacaccgag ccgaaatggt ggcatgcctt ttccgagaaa caaattctca
     2821 ttgcggtgga actagccaaa atactggttt cacagtatgc cttaaaggat attcttggac
     2881 atgacgacat tgcccctgat cggaagcgag atccgggccc cgcttttcct ctagaccata
     2941 tccgtgcatt ggtaattgga agatcacagg aggaaggtga ttggtatcag gtgatggcat
     3001 ctgcgttaaa tattcgctcc ggtcccggtt cggaatatga cattgtggcc tctcccctca
     3061 ccaagggaac gaaactcctc gctttggaga aacaggatcg atggacaaaa gtggatgtag
     3121 atccaagcgc actttcgtcc ggacaagatc tcgaaggttg ggtctttaac cgatacattg
     3181 aatccttgga cgcataacaa tcaggagtaa actgagaaat ctttcctctc cctccctggg
     3241 tcatcatcca gaagagattt agcaaatctt ttttacctag cccgggcaga ccttgcccgg
     3301 tgtcaaaatc cacacaggac aaatcaggta aaactctcct gcttttcaag ccttttatca
     3361 atttctttat cagcgatttc tggcacggtt cttggtctat tggatagtac gcacacgccg
     3421 aatcgttcat aattaacaag gggttgtcca tgggtgaaac cgaatattta gaccaggaag
     3481 atcaagaaaa agcagtagag attcgcattg aagatgtcat ttatcgctca gatgaaagcc
     3541 cggccgtcgt gctggcaatt aaacgaacct gttccctgag tggagaatgt aaagtcggac
     3601 atgtcacctt gggacatgaa aacacccttc gtttatattt ggaaacctaa tcatgcattc
     3661 actctcttaa atatattaag gctttaaccc aagactttca tccccatgtt ttcatccgcg
     3721 taccattttc atgtcctcaa ggggtattgc acatcaatat ccccttgatc agagagcaat
     3781 cggtaacccc acccaaatct taaccatttc acaagagtgg acaaggcggc tcacagaaga
     3841 ctcaccattt ctcctcctct ctgacttaat tttcaacctc aataatttca ggggtaaggc
     3901 cggtatccgt caaatgaaga cgggctacgg taatcggaag agtaaatcga cgagggccgg
     3961 cgctgccagg attgaggtag agtactctct tgcgtctctc agcagaggca cgatgggaat
     4021 gaccaaaaat gacagccgaa atttccgagg cgacagggtc tagagccaaa tcatccagtt
     4081 catgcaagac ataaaagaaa tatgattgat gttcgacgac attcgtcaga ggaatttcct
     4141 cagcccaagg gtcatgatca ttatttccac gaacagcgat caccggagcg atcgtctcca
     4201 gcatcttcag gacgtcaggg tttccgatat ctccggcgtg gataatcaat tcagctccgg
     4261 tcaacacctc aatcacctga gggcgaacaa gtccatgcgt atcagaaata atgccgatca
     4321 tattctcgct cttattggat gccctcatcc taagaattag gattcttttg tcggttgatc
     4381 acccattttc aaaatatccc cataatggcg ttggccacac tccggacaaa acccatgtgt
     4441 aaattgggca ttagatcgat cagtaataaa ttgttcgagc ggttgccaga tgttgttcga
     4501 atcacgaatc tttttgcaaa aactacaaat agggagaaac ccctccaata cgttgaccct
     4561 ttgggccaag gctcgtgttt gctcggcggt tcgatttacc aaaaatgcca taccacctag
     4621 caccaggatc ctgataatgg tgttgatcga agcatcaaac atggccagat gaatgggcca
     4681 tagcatgcca taaaaataca agcggataag agacatcgtc accgcaaaga atagtcccca
     4741 gaaaaatcca ctataccagg aggcgagaga caccggaata agatacaaaa tgggaaactg
     4801 aatggtcgga cctgctacgt aatccccaag taatattacg agtgccaacg caatcaaata
     4861 gccaggaaat aattttgaac gtgtcaaggc atctgacaaa aatcctgctc tggagaggag
     4921 taaagagaaa gctcggggag gcagcatagg cctaaacagg acagagtaaa aaagatcctt
     4981 ttcaaatcat cataccatag tcccatatgt cgagcatggg gttctgagtc aaatcattct
     5041 gaccagaaac cacctgatgc cgcactggga caattgaaga gaagccgcac tactccggaa
     5101 accggaccat ccccattggt aaacccaaca tggctcgcca gtcggcctaa cttctcaggg
     5161 atggttcact gaaggaggtc tccattcaga tgacgcatga attcgttatt tccttacggt
     5221 agaggagagt tacttatgga tagcgcttac tactggaaat ggtatagtaa aaagtatgtt
     5281 ggtcacggtt ttggaggctg cgtccattat cgcagccttt tttatcatga aaagaccatc
     5341 ttaggaatca agatccaacc ctcgttggaa tatttcctcc atggacacca atggcgaatg
     5401 ctacttccgg aaaggcaagc agaccagtga aaccgtccca tcctgttttt gatcaagcag
     5461 aagacttgaa agagagtccc ctcttccaac agcggggtaa tggccagacg cgaccaccca
     5521 cattatttgt catctttggt gcccagggag atctcacgaa gcgaaaattg atccccgcct
     5581 tatataacct ggcatcaagc caccatcttc ctcaagagtt tgccattctt gggatagatg
     5641 ggatccccat gcatacgaat gactttcggg agaagatccg tcaagatatc gatgaattgt
     5701 gtccccgtcc catcgatgcc acgatcaaag aatggctcct tgatcgtctg cattaccttt
     5761 caggggattt ccgggatcct cagacctacg aacgccttca ggttctgctg acacaattaa
     5821 ataccacaca cggaacccag gggaattacc tctattacat ggccacggct ccgacatttt
     5881 tttcggaaat cgtgcaacaa cttggggcca aaggattagt ggagtatcgc aaagaatttt
     5941 cacggcgaat tgtgattgaa aagccattcg gccatgattt gccatcggcc cgatcgctca
     6001 atcaagctct gaaacaggtg ttagatgaaa gtcagattta tcggatcgat cattatctgg
     6061 gaaaagaaac cgtacaaaac attttaatat ttcgttttgc gaacggcatc tttgaaccca
     6121 tctggaaccg tcaatacatt gatcatgtcc aaattactgt ggcagaaacc ttaggagttg
     6181 agcatcgcgg ggcctattat gaaaaagccg gtgcgctccg cgatatggtc tccaatcacc
     6241 tcttgcaaat tttggcattc gtagcgatgg agccacccaa tacgtttgat cctgaagcag
     6301 ttcggaacga gaaagccaag gtccttcgcg ccatccagcc catgaacccc gtagaggtct
     6361 tgcaatcaac agtcgccgga cagtatgaag ccggatcgat taatggtcat agcgtccttc
     6421 cttatcggac agaaaaggat gtcgatccag cctccctgac cgaaacgttt gccgccatga
     6481 ccttaggcat tgaaagttgg cgctgggaag gcgtgccctt ttaccttcgg accgggaaac
     6541 gattacacac ccgtgttacc gaagtggtca ttcaatttaa atcggctcca ctgatgctat
     6601 ttcgtaagac ccccgtcgac cgactcacgc cgaatgtgct ggtcattcgc attcaaccgg
     6661 acgaaggcat ttccttgagc tttggagcga aaatacccgg acccaaagtt caggtaggga
     6721 cagtagatat ggatttccaa tatgcggaat atttcggtga tgccccaagc acgggctatg
     6781 aaacgcttct acacgacgtg atggctggag acgcgacact ttttcagcga agcgatagcg
     6841 tcgaggtggg ctggagtatc gtcgatccca tcgtcaaagt atgggagagc ctgggttcgc
     6901 atgctattcg tccctatagc tcgggtagct ggggaccacc cgaagccgat gccttattgg
     6961 ccaaagacgg ccgaatgtgg agaaaccacg ggtaaccaga ccattgagta tccgggaatt
     7021 tcatttgcta ctataaactt ctcacgcacc agcctggctc acaggagtca gtatcatccc
     7081 tgaacgtggt ccaaaatcgg ctaagaccac ctttcccttt tcacacttga acttatttcc
     7141 cgtgaggtgg tcctcaagcc atcgagcggt cttccctacc cacggggcga gatcaatctg
     7201 aatggagacc ggatgatcat cattatgaat aaggatgaac acaacgcttc ttttcgtctt
     7261 tctcagaaat acacagcact gttgatccgg gagttcctgg atcaccgtcc aatccccctg
     7321 tcgtaacgtt ggaagagatt tccggattgc gacaagccgc ttggtaaaat cccgcaactc
     7381 gtcattccat ttgctctgat tccattccat gcccctacga ttgtccgggt ccttttctcc
     7441 ttccatcccg atttcatccc cataatacac tgccggtatc cctgggaaga caaactgaaa
     7501 cagcagggct aacttcagcc gttcaatatt tccttgagct acggtataaa ggcgttcagt
     7561 gtcatgggac cctaacaggt tcaccatcgc tgaggtcttt ttccatggat acatccgaag
     7621 cagtttgctc acgcgggaca caaacttcct ggttgaaatg gaaccttgaa taaaatagtc
     7681 caacacgatc tgacggaata cgtagttcgt tcctccgtca aattgtccat cccgaatcca
     7741 ggagcgaccg ggaccccaga tttccgccag catatacgcg gaggggttaa tcgttttgac
     7801 ttcatgccag agcgctttcc aaaatcgatg tccccggaca tcagccaccg catccaatcg
     7861 ccatccatca ataccctgat tcgtccaata tgctgccacc tcaagaaagt accgtcgtgt
     7921 ggcaggattg gctaaattga gaataggcat gaggggccgc tcttgccagc cccgataatt
     7981 aaatggtcca aatgcattga ccggaaatcg tgtgatgtaa aaccaggatg cgaaggggga
     8041 tgcctgacca cgctccatca cgtcataaaa gggaaagaag ccccggccgc aatgattgaa
     8101 tacgccatcc aacaatacgc gaatgtcatt ggcgtgagcc ttggacaaca atccctgaaa
     8161 gtctgctaat gaaccaagac gaggatcaat cctaaagtaa tcgtaagtat tgtatcgatg
     8221 attggccgag gcaaaaaaaa tgggcgccag atagatcaga ttcacaccaa ggtccaagag
     8281 ataatcgaga cgttcagtga ttccccggag agtacccccc ataaacccgt gggctgtggg
     8341 cttggatctc cagggtacga taccaggtcc ggacttagtc tcgtggctat ctcgcgcaaa
     8401 ccggtccgga aaaatctgat agaggatagc ctcccgagtc caggaaatgt ccgaagcggg
     8461 ttgatgattt ctcatggata cagaaccgga atcttgttca tgagaaagat gcgggcgtta
     8521 tggtacaaag aaaataaaaa aacccggagt cacaggaaac tccgggctag aggcggaaga
     8581 agaaattcgc tcagattttt ccaaaactga cgttcaacaa cacgagccga cgctgggaga
     8641 acaacagcag gaggatgcgc accctgagtg ctgacaacaa tccgcacaat cgcagccaca
     8701 ggtcatttgg cacccgtatc cgtgagatcc atgagaatag tcgccggcac cgtgggcatg
     8761 gccccaccca gcatctccgt gatggtattc agggccagct agcccttgaa ggaatcgtaa
     8821 tccgacaccc aaccccgagt ttaaaagatc cattcccaat ttcaaggttt cccctacccg
     8881 atcagcaatt tccccacttg tctcgctgac cgtttgtccc aaggccggat tcaaatgagg
     8941 catctgggga atctgaggca tgaaaccagt catagggggg aaaggccatc ccataggcat
     9001 ttgaggcatt cctgtcgcgc ccgtgccgaa ctgagggccg ccgtgttctg cggccggagc
     9061 atgttcaatt cgttttttat attcctgaaa ttccactttg gccttgttgg ctgcgccgac
     9121 attttttcct tttgaagaat tttttggtgt tgccgccatt gttatgctcc tggttaaacc
     9181 gattaacaac taaagacttc acaccatcga accctggcgg tcatgttgac gaccaagacc
     9241 gtcagatgag cgcctggttt ggttgtgata atgccttcct taatgacata gccatcctga
     9301 ggctcagcct gtttcaccca tcccagagtt gttttttcgc tgtcctgtac ggcgaccatg
     9361 accagggctg gggcagttga tgcggcattc gtcggagtat attcgatgga tagatgcacg
     9421 caacatccgc ccccgataaa agcttttcct attcccgctt ttcctcgcac atccacctcg
     9481 ccgacaattg gagccacggc agccttcgtc tctgcattct tgccgactgt atcgtcgact
     9541 gacagttcaa ccggagctgc aaacatggcc attgcccgga cccgctggag gaccatggaa
     9601 tgggaatccg tctttccatc cacattagtg tctgcttcta caagcaattc cttggcttcc
     9661 ttccgacatt cgcgatgccc gcaacagcat ttagccctgg gatggcaaca accaggacaa
     9721 aatccagcgc ccccatgctg aaaaagcggt ccggacatgt gaccaggtcc atggtgcgca
     9781 tgagtgggaa ttccagaata catcggttga gtgtaattca ttggcgtgac cttccttaat
     9841 cttgcctcaa attaaacagc cattcacaaa aaaataagcc gacgattgag tcatcctcaa
     9901 tcgatcaaca tcaacagcaa attgtttcgc accagcgcaa tcgggccatc gcttctttta
     9961 cttccaatgt cactttggca cctggctcaa tggataacaa ttgagatttc acatgatagc
    10021 cgtccgtcat ggaggtttcc tgccaggttg agatacttcc atcgatttca accgtgacct
    10081 tgaccaaggg ggaagtcgcc cccccatccg gcacgtattc cagggtcaat gaggccttgg
    10141 tcgttcctcc gatcaacact tccttcatgc cggttgcgct atccacactg acttcttgag
    10201 gaagaggcat ggaatgcgtg gcctgcccgc cccatccgta tccggcataa ccgactgagg
    10261 ggtaatacgg cattggcatc aggaccgggt accccattgg agccatgctg cgataaccag
    10321 tcgaatgaca atgcggtgac gaatggtggg acgtcgggac acagcaatgc ggtgaatggt
    10381 gatgcattag gaatctcctt cgttagagtc cgatgctttt gtcaaatcct gggtaatctt
    10441 caacacatct tccacaacct ggaaacccgt attgaccacg gtattgatca tgtcggccaa
    10501 tttttgagga atgtgattct ccagaatttg cgtggaagtc tgattaatca gtttgttaac
    10561 ggatcctata acggtggcca tggtgtgtgc ctttctccgg atgggaggga ttccctgccg
    10621 gtaatcaaat ggggcattta gtcaatgcgg ttcccgtcgg cgtcaatgat ccttcggcat
    10681 ggcctctggc caatcgaaga actttggtca tcacctcgag gctccagttt aaactttgcc
    10741 agggattcat cagcatacgg cttgacgctt tcgcactact ggtcaatgcg tcggtggcta
    10801 aacttaaagg cccggtcacc actccttttt cgtctaattg taacatagcc aaccaggccg
    10861 ccggattgcg gggcatggca tgaaacaacg cttgcgggac aagacaaata ttctgagaaa
    10921 gaaccccact gaacactccg ctacagaatt cttgaaattg ccggtcatgg gtggtcatgc
    10981 gagcaatgtt ggcaagaaca aataagcaga attctttcaa atcgggattt ttggccacgg
    11041 ataaaaataa ttctcccgtt cgccagtcca aatcaaattg ttgttcccat tgcatttggt
    11101 attgttgaag aacgcggacg gaacaatctc cttgtaacac ggcctgatgc gcagtttcgg
    11161 ccgctaaata cgccccttcc atcgctttat gaatgcctcc gccattgagt ggatcggctt
    11221 gattcgcggc atcacccacc agcatcaccc gatcggccac aatggccttg ggtttaaaaa
    11281 atgagacggc cccacctgaa acagctccgc aacgtgaggc atgacgcaag aggtgaccca
    11341 aatcattgtc aagaaaatcc tggaacacct gcttcagatt cgggatcatg ggaaacggct
    11401 tatcaaacgc gtaccccaac ccgatattcg caaagccatc atcatccaca aacacccagg
    11461 cataccctgg aaaaaaccgc tcatcgaaat acacctttat ttgggcgcct tcggcatcca
    11521 cattttggta ataggcacga agagaaaggg ccgtcgctcc attttttaag acgttcccga
    11581 tagagcgtga cacgacggag ctcacgccgt ctgccccgat aacaattttc ccctgtactt
    11641 ccgtgcgttg acctttatga tccaggacaa cacgggcatg atcgggtttc tggtgaatct
    11701 cccgaacaag ggaatgcgct tgaaatttgg ctccggagtc cacggcatgt tgaagaagga
    11761 tatgatcaaa acggcggcga tccaacaaca cgcaaaaatc cggatattga gtggaattcg
    11821 gaaatccccc ggtcagcagt aattcgccgt taataaagag gtcacacgtc ttaatacagg
    11881 atttggtgtg cgtcagcact tcgtcgatac agcccaactg gtcaagccag tacaacgctt
    11941 gaggggtcaa cccatcccca cagaccttat ccctgggaaa atcggttcga tcaaggatta
    12001 aggttgacag ccccttccgt gccgtaaacg cggcagtact gctccctccc ggtccggcgc
    12061 cgaccacaat cacatcgtac gtcataggtt ttcccagaaa atatccatat ggacagaaca
    12121 actctcactc aagagctttt ccctttacgt tgccgacgtc accaataatt tcaccggaat
    12181 ttcaatggcc tgttcggaaa ccacaaggat acgtccatga taaatgtccg gaggaagccc
    12241 atcgggaatg acacctttca acacaaattt ttcaaaatcc atcggattaa tcgttttcgt
    12301 tccagggttg accgtgaagc ctttgcaggg aaatacctga ccttgagtcg atccgataaa
    12361 cccgtcgact ttcaacttga cgttcttggc tgaatccatt gaatcattat ttatagaaaa
    12421 tgacacctgc acggattgcc ctggtgtcag cggcaaccga ttggcgatgt aataggcagg
    12481 gtcctgtggt tcgatacctt ctgattgttc cgaggaagac attggagcag ccggatcgcg
    12541 tgggctggag gtcggaggag cggattcctg aggtgaagcc gtggccgtac ccatgactcc
    12601 ggaaaatttc tccatcatgg aatcttgcat tatcgttcca aaacgattaa tcagggtcaa
    12661 tcccaaactt aaacccgctt cagccagatc caatcctttg gcaaacgtcg tgcctatttg
    12721 atcaccatgc ccgaacaccg aatcctcaga gttgatacgt tgatccgtat gagcagaggc
    12781 ttgggctgtc gacgtttttt gagctggaga cctgcggggt tgcgggttct ttttactgtt
    12841 cttaccggcc gtggctttac ttccggactt catgctcatg actccaaaga cattgtgcgg
    12901 attaacaaca tgaatggcac gtacagggtg aaggttggca ttgcacacaa taatggtgct
    12961 ggacaacggg tccacatccc tgacgcatgg tacatgcgca tccgtgggaa caaggtggat
    13021 cgcgccaaat aagcccgtgc atgaattgcc ggaaaaattg ggcaccagcg ccgaccagat
    13081 ggaaggcgag gccaagagcc tgcccagtgg tctgtcccag tatggtcgcc ccatctggtc
    13141 cgtgtgttga gtgtgaggaa ttcgaatgtg aacaggacat aggttaattc tccccgctgc
    13201 tgacagcact atccatgaaa tccctttgag atcggggttt ggtcatcgga aaagtatgag
    13261 aaggcacatt tcccactttc aacccacccg aaggggcttg cctccctcct ccgaatttac
    13321 ctcccggctt gagaccatct ttacctttta ttggtcgttc cgtgaatgtc ccgtgcattg
    13381 gctgatgaga gggagggatg ggctgacaag gtaatcccgt ggccacgacc cgtgaacctt
    13441 tagtcatcga tcgagatgga atagccggat tgccggcagt cgccacctga ggaaaacttt
    13501 gcgcgggagt acgcgattgc tcccgcctga ccctatctcg tggagaaacc atggtccgtc
    13561 cgtccaggtt gaggcggagg agttcaatcc ccaacacccg cgcgcgtaac gtatagctgg
    13621 aagggagaca aaaatcctga tgaagtccga tcgacacagg gggcaagcgc cggatattca
    13681 tatcaacagc agggagctgc cggagtgaca aattgacggt tggcagattg gtcacattga
    13741 actgttcaac acgtatcgga tccagattgc gaatttcgtc gattcgaaga gattcaatag
    13801 tcaggggatc aatatgattc agttccttaa tgtgtacagc ggccggggca atattccgaa
    13861 cttcgctgat cactagcgga tcaacattgc tcagttcact gatccgcaat ttgtcgagat
    13921 tttttgcttc tttaattttt atgtcgccca tataaatgcc cttccttggc tgatgctaca
    13981 ccagtcctgg ccagctatag tttaggtgaa tcgccttggc ctcatcgatg atggccggtt
    14041 gggtttgtaa tcgagccgaa attgtcacaa ctttggaaga gcccgatccg ttgagctgat
    14101 agagaaacca gggagcagtc cccgccccgc cctgcaccac tgagtccaca agccattgga
    14161 cagtcgcccc tgaactgtgc cgagtggtcg cccgaaataa ttgttgagtg ttgctctgat
    14221 caatataggc gttgtcatgt ccttgaggat aatagagata cgcctgcttg gggaaaactt
    14281 caagcgttgg ttgaggaagc ggctcgggtc catttcccat caaagtgaca aaagcatagg
    14341 ctttccgaaa agggctttcc gccgcagtgg cgatgataat gtcagttgtc ccatgagcaa
    14401 ggctgccttt cggaggagcg acatataagc cggtcggatc aatcgaacca gacccgggat
    14461 ttcctgcagg atcctgaacg gcccaattca ccttactgct catcccgtta tacaccgaag
    14521 cttgaaaccg gacctggcta ttcacaggat cgccatatcg ttcatcacga aaatacactt
    14581 cagcgttgac cggccaaacc tctacttcaa tttgatgctg acagacgatc tgatggtaga
    14641 cctggtaggt cggatgggaa tgacgacatg gtgcagtgtg aaccggcacg gctaaccttt
    14701 ctctatcatt tgctcacgag gacatctaca ttgtatcgac gtgttcctca aagtcatcgt
    14761 ccgggagatc aacggaacaa ttggcaatct tcccgaagag tttgccattg aagcttagct
    14821 tggatttaca tccaaccttc ccctccaatt gtgtcgtgat cccttttgtg ccaaggactc
    14881 cggctaattg cacgttggcc ccttcaaccg cgacggtaaa tggagaaagc tttataccga
    14941 aactgccaat cgaagccacc gtatggattc caccggcccg ccggagaaac ggaatcgaaa
    15001 gattgatcgg gatctcgctg acctgcaccg aaattgaact cacctgcgcg acaaaagatc
    15061 ctttggcctt tgtggcaaag gcgattggtt cggttttagc ctggacagaa aaatccgggc
    15121 aactctccat acccagttgg cctttgaaat tcacgtagac tcctcctgtg ctgtctgttg
    15181 agtcgtcatc ccgtccggat agacccgctc ggccagatca ctacacattg catcaatccg
    15241 ttcattcaat tgttcaaacc ccttcaagga ttgacaaatt tcttcacagt tttccccttc
    15301 aatctcaaac gagacaaagg gaccaagttt aattcgaaga accatattgg ccgccattct
    15361 atcacctttg gtaaaaaagg aaaccaggga gtacaaataa gggtgttccc ttcaatacat
    15421 tgcctcccct taatttaacg tggccaccta ccattgatca tttcgacttc cttgaatcct
    15481 ggcgccgcac cggacgcttg ctgtttccat tcaccgcttt catcgttccg gtctcattgg
    15541 ctgtcttttt cgccatacca tggccattcg tctggacgtg cccattacca ggcttgtcgt
    15601 tcacctgact caggtttcta tctgcctctc gcaagaattt caccatctga tccatggaat
    15661 gtaacatgac ttcaagcgtg tctttttgtt gccgatcaag atttttgtat aaatcttcaa
    15721 gctttcgttc aagttcttga ccgtcactgg aagtgcggta ttctttgcca cgttcccgta
    15781 gaaccgtccc gaactgatcc aggatggaga tcacaccaaa taaaatatcc aacgttacgt
    15841 cctgtccttc ctggccacga ggatatgggt gcggacggca atgggaacaa gccagaacct
    15901 gttcatctat tcccatactc catctggcga aaagagccga tcgggaatcg tcagcaatat
    15961 tcgaacattc ccgacgatga agtttcccat aaacaggatc gaattgatac ggggccatgg
    16021 ccgcttcggc ttggccatca attcgcaaca gaggacgcgc atcgctgacc tttccttgag
    16081 atcgtagctg ctgaagggca atcgcaaata catcacccca atacacttct gtggttcccg
    16141 atgcatcctc aggagaacgt cccataactc tatgtacgcc tgaccaccgg gcttcctctt
    16201 ccttaaacgt ttgcgtcggt gaaaatggaa caaaccggac acaccatttc ccaaacggtc
    16261 atgatcattc ttcctggggg cttaatcata gactgcccag gccaactctc gatgtgtctt
    16321 ttgcaaacga tgctttttga gacgtgtcgc aaaatatttt gtcaagaagg caaccagcaa
    16381 atcccgcccc gaaccttctg gtagcccctg acgcaaacgg gcaatatggc cttgaaagat
    16441 ccgacgaaga gtgcgttcgg ggtcggcaac ctgattgaga taggtcaccc ggttataatg
    16501 caagtctgaa tagcaatccc cgtgatcgtc gatatacata atcaaagaac ctgcgagaaa
    16561 gatcatactt aaataatgca gaggaaccgc tgggactaaa aatcctgtta gaactaaaat
    16621 agcatggccg cttcggttcc tggcactttc ccgtaagact cgcaattgtt ctcggcgagg
    16681 gcgtgagcga atgctctccg gatcagagcg gaaagccaca tcccgttttt gcgcctgaaa
    16741 tagcagcagg atggcttgcc gaaggaaagg atccaagaca tcccgttcag cgaacaaacg
    16801 agcaataatc acataaaaca gtttttctcg atccgtttga atctcaaacc aacccttggt
    16861 caaaaggtca tccatccgat caatatagga atgagcaaca tccaagccaa agtcatcaat
    16921 aaagctatcc gcaaggaaac aacaaccccc gaacaaactg ccaatcattt gccgacgcgc
    16981 atcataggga aatcccgcaa tttccgcgca ggcatgtccc acaacggtca tcttggcact
    17041 cagttccatc atcttccggt attcatcctg gcgttgttca aaaagatcgg gataatactc
    17101 cagcactaac ggcttcagaa agaccaccac ctgtttccga taaaaactga acaattgatc
    17161 aagaggcgta tggagggatt gggacgcgta catgaaaaaa acatctcgtt agaacgaaag
    17221 ggttcctatt ataatgcgtt gaagcgaaac tttaaatatt tcccaatgaa tattgagaaa
    17281 aaatctaaaa attaaatcag agctatatga tgcaacggtt atgccttcag gaagatatgc
    17341 aaatgtgaag tgtgagccgg cgaagattct tgtctcacca aaaatttcca ccaaatgaaa
    17401 gggttttatc agaattctgg caaatgcggg ctctaccgga agaaaagact catgggatcc
    17461 gtgagggaat gttgtaggct caacttcact gcactgatgc aaaaagcctc aacatcatac
    17521 tttgaaatcc cttagaagcc gagatacttt acgacacaaa aacaattggt gggttgcagg
    17581 ttgtccaata caaaacaaga ttggatcaga cttcgaagtt gacagaaaag ttattttctc
    17641 ctaccaaaaa ctcaatccgg tttggccact ggattttttt cggcgtctgt agagccatgc
    17701 aattgagctc tggtgtctag ttcgtgggct tttcgcccgc ccctcgcggt ccggcattcg
    17761 cctaaattcc catgtatcat cacgctggcc atttgaacac agcgttctac cagggcagtt
    17821 gcgccctccc tgagtgattg ggacttctgc acatatcgca tattattttg catgtcttcc
    17881 gcactccatc ctcgggaatg ggcaatatag ggaaactgcg ggaactgcac accgatttcg
    17941 gcgaaaaacc ccagcagttg tcccgccaca gcctgaatat tatcctgccc acccgtaatg
    18001 ataaacccgc ccactttatt atgaagcaga tgtgtatctg aaatcgtttc ttgattctgt
    18061 atgcagttca tccgctcaac catcttgaaa taaaggctgc tggccgcccc ccaccgaatg
    18121 ggagtggcca gcaccatgac atcagcccaa tgcaccagac cctcgtagac ctggtccatc
    18181 tcatcttgtg gatccatctg cgtaatcgaa cagggccagg tacaggcccg tgatgatttg
    18241 gaataaaaac cctcgcaagg gcgaaaagac aaatcccgaa gacgcaacaa gcgggtttca
    18301 caatctagat tctccgaggc atgcttcagt ccgacttcaa gcaaatcttc cgaggtacta
    18361 tagcgaggat gatcaggcgt cataaccgtc gtggaaagcc cgagaacccg aatcggacct
    18421 ttttccctgt ggggagttcg agcaagcgga tggggttcat gtgctttccg agttcgtttc
    18481 gaaatcgccg tggaccgaat caagactcgc ccctccgcta ctttcacctc ataggccggc
    18541 accgcgtctt cttcatatcc cggttcccct ttgcccgttc gataatgaaa cttccaataa
    18601 tgccaggggc agaccacgta ctccccgtcc aaggttcctt cacccaacgg cccacctaca
    18661 tggttgcacg cgccattgat cgctccgaac tccccattgc ggtaggttaa cgccactggg
    18721 gttcgtccga tcagcacctg ctgcaccggc tgatgcttca aatcttcgac accacccaca
    18781 tcgatccagg ttgaattcgg catgttgact cctggtaaaa atgaatcgtc aatctttttt
    18841 taacgaaaaa aaacgattat cacaaactgg aaagaccgtg cgaccctatt gcggaggcaa
    18901 atctctgcct ttcgaatcct cgacgattaa tataccgccc ataatcggat ggatttggca
    18961 gtggtatgaa tagcggccag ggggtagccc aggaagtgta tatgttttcc ccggtgcaac
    19021 cttcccggaa tcaaacagac acggtttccc tgttccacaa tcgtcatgtt gaacggtatg
    19081 aggagacgcg gtcgcattaa tccactctat gggaacaccg gcccagacca cagcttcatg
    19141 tggataataa tagggaggaa aatgttccat ccggatcggt tcattcacaa attcggttcc
    19201 tgatgtgaca gtgcctagca agacgatata gagaaaaatg acacactgga gtgttttcag
    19261 aggatatctc ctttccgatt gtatggtttt agggattgta ctgtgcctac tagtctcaac
    19321 caaggtctta aataaagcaa acagtggcaa ggaggttccc cggcctctcc tttctcaaat
    19381 cctgagctat tagccaattg tcaccttctt cattataact gacgcagctc cgggatttgg
    19441 atgcctcaca cagaaagaca gggaatgtcc caggggagca tatggggatg ggaaaaatga
    19501 tgctgaatca gtccctcgtc atacaatgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn gagcatatgg ggatgggaaa aatgatgctg aatcagtccc tcgtcataca
    19681 atgctgatcc cgtgaggcac ggatcgaatc gttccattta taagcccact tgattcctaa
    19741 gccaaacgga atagaatagt atcaccttcg accggaaaga aaagtcagga ctgaaccgag
    19801 tgcttcgctt tcctttcaaa atcttccaca gacaattgcg aaggatactc gttgcctttt
    19861 tatccgttgg gaccttcgtg attgtggatg gaagtccggc tcaagctcaa acaccaccca
    19921 ttacttcatc cggactcaat actcaggtca gtgcgccgag gcatcttccc ggcggagcgg
    19981 ttcaacatga tattaccggt ggaacgagac cgggtggcgg aagtaatctc ttccatagct
    20041 ttggggattt tggtgttccc acggacaata ttgccaactt cctgaacgat tcgggacttc
    20101 cgacttcaaa catccttagt cgcgtcaccg gcggcaatcc atccaatatt tttggaacca
    20161 ttcaaaccaa aggtttcggc aatgcaaatc tctttttaat gaatcccgcg ggaatagtct
    20221 tcggccccaa tgctgccttg aatgtgggcg gggctaccca ttttacgacc gctgattatg
    20281 tgcggcttga agatggggcc cagttcaccg ccctgcccgg tgctcaggat tctcttctca
    20341 gcattgctcc gattgcggcc tttggatttc tcggtccgaa tccagccggc atatcggtcg
    20401 aaggtagcac gttgtcgata tccgaaggcc aaccgctttc gctcataggc ggcgacatca
    20461 cgattggccg aagcaatctc actgcacccg gagggcagat tgccctggct agtgtcggct
    20521 cgccgggaga aatcctggcg aacacttttg accagacatc caatattcat gggcatacca
    20581 tggggtcggg tggcactatc aggatctcag accagtctgt catagatgcc ggcggagagc
    20641 atggtggcaa gatgttgatt cggggtggtc gattggtcct cgaccatgaa tcccaaatcc
    20701 gtgccaatac gctgggagcg caagacggtg gccatgtcga tattgccgta agcgagaatg
    20761 tcctgatagc gggtacaggt gcgcaaacgg acaccccgag cggaatattt gccaaaacag
    20821 aaggcggcgg aaagggggga acgattacga tcagtgcaag ctctgtgaag ttgactgatg
    20881 gcggaaacct gtcagtgagc acccgagggg aaggggatgc gggaaccatt atggtcaatg
    20941 cccctgatgg caaggtcatc ctatcaggga gtgataatcg cttgactgat ttttcgcctg
    21001 aggaagaggc cgtgagcggc ttgtttgcca acacagtggg aacaggtcgg gcgggtacca
    21061 tcacggtgca gaccggggat ctcactataa gtgaaggggc tcgcatttcg gccggcacct
    21121 ttgggcgagg caacggcgat gcaggaaaac tctttattgc ggctacagga gacgttcttc
    21181 tttcaggcac acaagtcaat ggcatcacct catctggtct ctttgcgggt gcaagtgaat
    21241 tgactcttcc cgggaatgga cttctgggtt ccggcggaac gattaacatg accggtcaaa
    21301 ctgtccggat cctggatggg gcgaaaatac atgtcgcctc aataggacag gagcaaccgg
    21361 acatgaacag gctcgataat gctccatcgg ccgggaccgt gaacattaag gcggccatgc
    21421 tggaccttac tggagcggaa ttggacggaa aggtcctcga cattattcca agtgagataa
    21481 cagccagcgc gactggcccg gcctcggcgg ggcacattct gttggaagca gacaagatca
    21541 cgattaaaga tggagggggc gtcacggcgg atacggaatc cataaccaga atagaaaaaa
    21601 ctgggggtat ccagctaaag gcaaaggaaa atcttgtcat ctcgggaacc gcggttcgaa
    21661 cggagtttcc accgaatctt cttccacaaa acacacttaa cccccgaacc gtcgttcaga
    21721 gtgcagttcg tgctcagaca aagggcgtga atcgcggagg cgaaattcac atcagtgcag
    21781 aaaatctatt tgttgacggc ggaggcctca tctccaatgc ggctcttaat ggagcgggag
    21841 ctggtggaaa catacacctt gacgtcgtag atacgattcg attgtcaggt gcgtacgaaa
    21901 aagtggacaa gaacaggaca atttttagaa gtgagatttc tagtgggaca gaggggcccg
    21961 gtcatggtgg tgacatttct atcacggcca aaaatgttga gatgctggat ggggcatgga
    22021 tttcatcaag aacgagaggc cggggaagtg gtcatgccgg ccgggtgagt atcacggcag
    22081 aagaaaacgt ttctctctcc ggattcatga gcgtatatga tccagaaatc aataacacca
    22141 gcaaaaccca ggaaatatca agtcgaatta ttgcacgcgc agaggaacac gacaatcggc
    22201 tagcggattt acctcttggt aatgctggcg atatctcgat tacagcgaaa aatgtcgtga
    22261 tgaaagatgg ggcggaaatc atagccccgt catcctccgg aagagtgata cccataacgg
    22321 atgccataga ggaaatcgtt gccaacatgc cagaagaaca gcgtccacgt gcagggaata
    22381 tcactgttaa agctgataca ataatcctat ccggcgcgcg aacggttgga ggatcaggaa
    22441 aattcggttg ggctccaacc accatccgcg ctggggttat cggaccggca ggaaccggga
    22501 atgtttcact gactgccaat actattgtca ttcgtgatgg ggccgatatc agcgctgaaa
    22561 ctcaatctag cactcaggga gttcagcggg gcaacgtgac aattaccgct catgacagct
    22621 taactctctc aggatatctt ttgcgtacgg aatttccaga aaaccctata ggatcagacc
    22681 cctttcctga agccaatggg cggacgatca ggccgaccag cattgttgcc cgaacgagtg
    22741 ggacaaatca ggctggggat atcattctct cggcaaaaaa tctcataatt actgagggag
    22801 ctcaaattgc aaatgggacc ctcaatcatc agcttgacaa tgcagggggg ggtgggaaaa
    22861 tccttctcac agccgaccat ttgactatat ctggcatggc cccattccct gatgccatcg
    22921 gaagacccct gccaagtatt atttcaagca gaaccgatgg gcctggtcaa gccggtcaaa
    22981 ttgacattac ttcgaaaacg atagaaatca agagcggggg gcagatttcc gccagcagta
    23041 ttccccgcga ggatgcgatt ggcgcgggag caggaggcaa tattcaggta gtggcgaccg
    23101 aaagattcct ggcaaaagga gtgggaattc ttgagggaac cagaggtgtt cgaagtggaa
    23161 tctttagtag cacggaagga actggattag gagggaattt gcggattacg gcccccgtga
    23221 tcaaaatgaa aaatggagcc gctattagtg catccacgac cggcacggga aatgccggag
    23281 atattttcat tacggccagt gactcaatcc tgcttgacaa ggcttctgtg acaacagagg
    23341 cggggtcggc ggtgggtgga aatattaaac tcacggccga taatcgcatt gatgtgatca
    23401 acagtcaggt gactagccga gttagggaag gatcagggtc cgctggaagt atcaatttcg
    23461 acccagactt tatcgtactc aaaaatagtc aaatattgag taccgccgtt gcaggatctg
    23521 gaggtgatat caccctcacc gccaattcgg cgatcgtgat ggacccattt acattcttag
    23581 acgcgcgttc gcggttcggg ggcaacggca gggtcaacat tgaatccccg gttcaattcc
    23641 tgagtagtac cattgtgccc ttgccgcaaa atctggttcc ggtgacgact ctgtatggca
    23701 ccaaatgtgt ggcggagcaa ggtcggttta gttcgtttgt agattccaag gccgactcca
    23761 ttgcgccgac tcctggagcc ttgttagcca gtccccttct tccgttttcc tctcattctg
    23821 ttgcgtcgaa tccgatcgat attcatgtag attcaaacaa tcaaacacag gctgatccca
    23881 ctcacgcgac gacatattcc ccgccggttc tctttagcca ggccactggt cagcgtgcaa
    23941 cctgccgtcc gtaaccacta tgaaacacct atccctttta attcctgaat ttctaaaata
    24001 gatattcttt tccctggttc gattactctt tgccatttct tcctcctgtt cataaaagga
    24061 agtcgggcta tgaatacgta tttccctggt tatagagata cgatgtgccg cgttttctag
    24121 tttggaggta gtgccttgaa aaacgtgttt gcgcaggagc ttcagaggtg gaacaagtgg
    24181 ttgaaactta catggccgat acttttattg aaccgggaca gccccttaaa ggaaaagaac
    24241 atgcaagagg acggcacaga gggagatagc agatatggcg atcgccgcaa gactgatttg
    24301 caaggcgcgt tgctgattag atgtggccac tgaggcggcg tgttcgattg ccgcaaccgt
    24361 actttgcaat tgttcggcga gttctttgac atgtgtggcg ttttgggagg cggctgtttg
    24421 taactcgctg atctgttgct gcaacaaatg tgtctgatcg gttgtagggg cggagtcttt
    24481 cttggtaaac gctggtgcgg tagccgaaat gattgtcccg atatgtggaa gaacggcttt
    24541 aagtgccgga ataagccatg ttgccataag attacctcac aacaattaac atctattagt
    24601 ccgatgaagt accgaaagca atatgctaaa aatgtagcgg tttgtcaaag aaatggccat
    24661 aaagctcaaa agatgttcta aggagatttc cctgtgccgg gtggagttac ggttcgacac
    24721 caatccactg tgggagatct ccccacctta tccaaacagg gcacgctcag ctcgctgcag
    24781 atcatcaggc gagccgaata ataccacgac atcccctgcc tccaccctgg tttcgcctga
    24841 cggcatgagt tgtcgttgcc cttgccgtac caaagcagca atcgcaacgc ccttaagatt
    24901 cagttcctgt aatgagtgtc ccacaacaga actgtccgga ggtagcacga ccggtcgaag
    24961 gcggtcaacg ttgtgttctt cccgtgtgtc agccagcagg ctatcgccca gaaagaactc
    25021 acgtaataaa tgataacgcc ctttgcgctg ttcccgcatt cggcgtgcca cgcgggaaat
    25081 gggcacgtcc agtaacacga gagcatgtgc ggcgatcatg agaccggctt ccagcgtctc
    25141 cggcaccacc tctgttgcgc cagccgcacg caattcctcg acgtgggttt catcgcgggt
    25201 gcgtaccatg atcggaagat ccggacgtag agtccgaagg tgatgcagag ttttatgggc
    25261 cgaggctaca tctgcatgcc cgatcacgac cagccgggcc gtgccgacac caatcccttc
    25321 gagaatggta cgttcggacg catctccgta aaaaactccc tcgcctgcga tattcgcctc
    25381 ctttaccctg gacggatcca gatccaatgc cacaaaagga atctgttcct cttccagaag
    25441 atgagccaca ctctggccaa tgcgtccgta cccgcaaatg atgacatgat cgcgaagatg
    25501 tccgactgtt tctatgctga cttggggcac cgcatccttt ccctctcggg caggacgagg
    25561 ggcacaccag cgagccagca cctggttgta ccggataaga aatggagcaa caatcataga
    25621 aaataataca gaggtcagca caatctgccc gatttttggt tggattacac ccacgtccag
    25681 cgcgatagaa agcaaagcga atccgaactc acctcccaca gccagcagcc aaccggtacg
    25741 ccaggccgtc gtcgcgtcca ttccggcaat gcgtaccatc agtgccacga gtaaggtctt
    25801 gatgagcaac aagaccgtgg ctcccgccaa ggccaaatgc caaatttcag ggattgtcga
    25861 cgggtcgacc aacataccga tcccgatgaa gaatagacca agcaatacgt cgcggaaagg
    25921 acgaattgtc gattccacct gatgtcggaa ttcggtctca cccagcatca tgcctgccag
    25981 gaacgcaccg aacgccatag acaggccaag gctactcgtg gtccatgccg agaccaatga
    26041 cacaaatagc actgtcaggg taaacacttc cgtggagcgg agctccgcga ccagataaaa
    26101 caacgggcgc atgacccatc gtccggccag aaagaccaat ccgaatgcca gggccgcttt
    26161 cgcaaatgcc attccgagcg agctggccag aaccacgacc tccgtattcg ccatagcaag
    26221 cacagggatg acgaccacga aaggaactgc tgtaacatcc tggaagacgg acatggctat
    26281 ccccaaccgt ccatgccggc tatgctcctc tccctgatca gtgagttgct tactgataat
    26341 ggttgtggag gactgagcga agaccgctcc caccacgaag gccgccgcaa tgggcatccc
    26401 cacgagccaa gccaggaggc ccaccgcgat cgtcgtaagc aggacctgtc ccgtgccaag
    26461 acccagtagc tgatgacgca aggcatggat ctgcggcatc gagaagttca ggccaatggt
    26521 aaacaataaa aagacgatgc cgaattctgc cagtgcgcga atgggctggg cttcaatcac
    26581 cggcccaggg gtgtacgaac cgagcagaag gccaacgagc agataaccaa gaggcgaagg
    26641 aatatgcaac cggtggaatg caactacaac aaccacggct gttccgagta ataaaagaga
    26701 ctgcaggaaa aactgctcta tcattgggat ccttttggaa ccatcgatgc cataccggac
    26761 taaaattgtt cttgcgtcac aaacaatccg ccaccaatta cttacgtctg ttccgaagat
    26821 actccgaaac ctatcagggg aaacgtatca cgacggacgg tgtcgagtcc taagaaggac
    26881 aaaactcatc ctgaaaggat aaaaccggcc atgcgggcta caatcaaaga tgtagcccat
    26941 gagaaaccat gccaacaata gttactatta aatatgatga attggcaaga gccacttgtg
    27001 gaaacatctc attccaacaa aaaatgaatg taaccttcac gagagattca atagcgaaaa
    27061 taaacgaccc ccggtactgc ttctgatgtt cattggctag tgactccccc tgcacctact
    27121 tttttacaaa caaatatatc gccaggccga tgagaatgac ggaaaagatt tttttgaaca
    27181 tgtcagtggg gacaccttca ataaggcgag caccgatttg tgccccgatg attcctccca
    27241 ggccgagcag gaggccatat ttgtaatcca cgttagccag cctaccgtgg gcaatcaagg
    27301 ccgagaccgc aatcaccagg atcccgacga atgaggtgcc aacggctttt tgtgccgtga
    27361 agcctagaaa aaggagaaga ggaaccatca agaatccgcc gccaagcccg gtgaaggaag
    27421 cacccatacc cacaaatacc ccgctcgcaa taatggctaa cgatgtcaca tccatgaaag
    27481 ctttcctttt cgatcttgtg tgcaaccaaa gcaccaaatg ttacaaaaaa ccatgaagtt
    27541 gaatgacagc gaatttaacc ggaacaggta attttaaaat tcttttttct cctttcctca
    27601 ccccaggact aatacactta aaagagcgga aaaggtcgaa gatggaatga gtttgaagtg
    27661 attcaactgt tcctggccca tatctctgga aagagaccac cccattcccg taagattgca
    27721 aaccacaaca agccatcaat tgagggtttc ctgatttcaa taatacccca cccgactgtt
    27781 tggcgaaatc ttaaaatgaa ttcactctca taatagaaat gaggtttcca tgagttctgc
    27841 tgaatttgac tcttcccaac caatttcaat ggctcaattg gccgatcccc atcttcttta
    27901 tcaaaagagc gtgcaaagcc ctgaacttga catgccgttc ctcactgaat atttcgagag
    27961 ctataccaac actccccttc gccactttcg ggaggatttc tgtggaaccg cctttttatc
    28021 ctcccatttt gtaaccaggc atcccgacaa tcatgccatg ggcatcgacc tggattggcc
    28081 cacgctgaat tggggcatca aacacaacgt ttctcatctg actcccgagc aacagcagcg
    28141 actcacattg gtgaatgaca atgtcttgaa cgtccaacac cccctctcgc aactgaccgt
    28201 cgccatgaat tttagttata tggtttttcg tgaccgcccg acccttttag aatatttcag
    28261 gaatgccagg cgatcgattc agcccggcgg gttgtttatg ctggatatct gggggggcag
    28321 cgaaacgcag gtggaacaag aggaacatcg agacatcgac aatccggttg atgacggcat
    28381 cggcgatttt atctttgtgt gggatcagga tgtctttgaa ccctccacgt atttctgtac
    28441 aacccggatt cattttcttt tccgcgatgg cagtgagctt cgcaatgcct ttgtctatga
    28501 ttggcgcttg tggacgattc ccgaagtcac agaactcatg aaagaggccg gttttaaaga
    28561 cgtgcacttt ctctgggaag gcctcaatgc agaggcaaat gaagggacag gcacctatca
    28621 tcgcgtggag aaaggtgatt ccgatccatc atggatcgcc tatctggttg gccagaatcc
    28681 agaataaggg atggtgtcag ccaaactatg gctctttccc tttttgaatg tgagtctatt
    28741 gtcggagaat tgaaccgggc tcttgtcggt gggttcatcc agaaaatcca ccaaccccgg
    28801 ctccttaccc tcaccctcga tgtccgaaca cgaggacaga cgagtacctt actcgtgagc
    28861 gtggaacccc ggtttgcccg cctgcatctt acctctcaga agtttgagaa tccgccgact
    28921 cccccaccct tttgttcttt tctgcgttcc cacgtggagg gtgggcgcat tgtggagatc
    28981 agccaggagc ccggggaccg gattgtgttt atcaccattg tgaaagccga gcaggtgtgt
    29041 gttttggtga ttgcgttgat cggcaatcag gcgaatgtgc atttgttgaa tgagaaaaaa
    29101 ttgacgttgc ggtcattacg ggaatcgcgt gtgaaggtcg gagagcgcta tgcgccccca
    29161 acattgaggc cggtgaaacc gccggaggct ctccctcccc cctccatggc tggacgggtc
    29221 cctcagaacg ggatcgttat tcagcaggaa ggcgaaatga gtgaaccgct ttctgaatcc
    29281 tttactgaag gaaaaggagc gtttagggaa atatttccgg tgtcggctga actggaagcc
    29341 cgatatggcc aacaagaaca ggaggaaggc caggatgcca ttcttgaaca gcaactgttt
    29401 caggctcgaa aggcgctgaa gcatgcgaaa agaaaaattc aggccttaca agaagatttc
    29461 aaaaagacgg agcggtttcg ggaatatgcc cggtatggag aactattgaa aagtcatctc
    29521 catgaaatca aaaagggaca ggagagcatc gccatggttg attattatga tcccgccttg
    29581 cccactctga ctctccctct tgacccatct aaagatgccg tgtggaatat ggaagattac
    29641 tttcgaaaat atcgtaaatt tatcggggct caggaacatc tccaaccccg tgtggaccat
    29701 gcccaacagg agatggcccg attggaggca aaattggcgc agttggaaca agggatcgta
    29761 gattctgatt tttcaccaaa acccagcaaa acacgaggct cctctgttcc ttctggcact
    29821 cgagtatcca aaggcaaacc ggctccagcc caagcctacc ggacatatac ctctgtcgac
    29881 gggctcccga ttttagtcgg aaaaacagcc aaggataatg atcatctgac attgaaggtc
    29941 gccaatgcgg atgacctgtg gctccatgca cgagggacac caggctctca tgtggttgtc
    30001 cggttggaga aaggcgccac ggtgccaccg gagaccttaa aggatgcggc gacgttgacc
    30061 ctctggttta gtgatttgcg aaaaagcggg aaaggcgagg tgatttatac tctgcggaag
    30121 ttcgtcagga aaggcaaagg cttcaaacca ggttccgtca cggtggagag agaaaaatcg
    30181 ctctggattg aattaaaaga agatcgattg aagaggttga agggcgaaac aagccctggc
    30241 taaccatgtc atccagatcc attcgaattc gctcaggaaa aggtccgacg agactctaaa
    30301 aaagtcgatc atcgtcaacc gtggcacaga cccttcgccc gtgaccgata accaggaaat
    30361 tattgacttt tccaatccgc cgtttctcta tcaagaacaa actacttctc tgagaacgga
    30421 gccattccta taaaactgtt tcccccttcc tcagtcttgg accggcgcca cgacagccta
    30481 tgaatgagat gctgggctaa ccgaaggatt aaacccagaa acacacaaga tgaaaacgaa
    30541 aagaaagtgc ctgcaactcc ttcaagaaat catcttcaat tctcacactg cacaaagcac
    30601 tcactaatag ggtgcatgcc ctcaccccct tttccgtatt gcctgacttt tctgacttaa
    30661 ccactaaaaa aaaagaccta aaaatcacga ttattaccac agaaaaaaag aaaaacgaaa
    30721 acagttcacc tctgaactgc gcgatcttca catgaagcgc taagttcgtg cgtataggaa
    30781 attcgaatcc aaccgatagg atgagctatc ggagggggaa atgcgaaata tttgtacgct
    30841 tggccttgtg ggtttcttcg tactccttgg tggatgtgcc tcgaatcata cggccaatga
    30901 tgagcagatt gacctctatc tcaacacact ggatgataag catttcgtct ggtgcgaatt
    30961 agatctggag caatgtcgac atgactttga gcaatggaag gataccgcag gaggtcgcag
    31021 gataattcag gaattcgaaa aagaggatac tggtcaaacg gacaatattc agcacttgcc
    31081 gcatgtcttt cgtacgcggt ttgtggatga aggccagtta ggagaggagt tgctaggagg
    31141 agagggtaag gggcaaactt tcggtcagga taccgaagga ttggtcaggt ccgattcgag
    31201 tatcgatgaa aatgaccatg ccacacaaga aggaatgagc atgattccaa aaattcagga
    31261 tccccacttc gtgccccaaa taccttgata acccattagg gaataaatca ccaagacagt
    31321 gagtttttcc ggttcgttcg cagacttccc gctttcgctc tgatacagca gatttaacct
    31381 caccattcgg ttcagatcca tcccccgttg ttagtcgatc atcattcgtc tccaccttgg
    31441 aaagagcaaa cagtcgtgta accaacagta tcttcaggac tgcgccccta cctcgatggt
    31501 ttgcacctga tccggtaggc aatcggcaat catgctcggg tggacttcag aaaattttca
    31561 ccttcagcgg atcggcgcgc agatatcccg aacgtggccg cgcagccagc gatgcgcgag
    31621 atcggaatcc aggcgcgggt gccagagcag ggaaactgtg acctccggta gagagaccgg
    31681 aaggggaaaa ctatacatcc cggcacgcaa gtttcctgtg tgtcgttcag gaaccgtggc
    31741 gaccaggtcg gaggctcggg cgagagccag cgccgtcgaa aaaccaccga cgatcgtgac
    31801 aatttttcgc tccagcccga atggctccaa ggcttcatca accgatccct tgtcgagacc
    31861 ccgccgtgag acgacgatgt gcttcccggc agcataacgg gagggcgtga tcttcccctt
    31921 gctcagcgca tgccccattc gcacgacccc aataaaccgg tctcggaaca aagcctgcgc
    31981 gcgcacctcc ggagcagtcg tatcggccac aacgccggtt tccagatcga cggtcccgtc
    32041 gcgaaggggc gcgctgtctt tgttgggctt ctgcacgaag cgcaaccgca caccaggagc
    32101 ttcttcgcca atgcgggcaa tgagggccgg tccgaagttc tccacaaagc cttcgccggt
    32161 ccggagcgtg aacgtccgga cgagctgttt gaggttgagt ttctcggcag gccgtagaac
    32221 ggcttctccg tcctgcacga gttgcctcac ccgctcgcgg agttcgagcg ctcggggtgt
    32281 gggaacgagg ctgcgtccgg ccctcaccaa cagcggatcg ccaactgtct cacgcaatcg
    32341 cgccagcgcc cggctcatcg ccgacgggct cagccgcaac cgttgggccg cgcgcgccac
    32401 actcccttcc gcgagcaccg catcaagcgt gacaagcagg ttgaaatcag gggtcgacat
    32461 gcattgattt tagcacagtt tattcctgat gtagcgtcac acgcacgaat aaagtgcaag
    32521 tggtgcgtct tccgccatat caaacttgag ggtatatttt ggatagcaac atatcgggag
    32581 ttgccaaaaa gtgggagcct atgttgaagc caaccattgc aacacaagat gtgatcgtaa
    32641 ctggaagtgc agaacagaca ccgtcaattc ggtgggcgct catcggcctt tccctatcca
    32701 tgttgctatc ttcactcgac acgagcatcg ccaatgtggc gttgccgacg ttgacacggg
    32761 ccttcaccgc ctccttccag gaagtccaat gggtaattct cgcgtatctc cttgccgcca
    32821 cgactctgat cgtcagcgtc ggacggctcg gtgacatcat tggccgacga cggctgctat
    32881 tagccggaat tttcctgtac acggtggcct cggttctctg cgcaaccgcg cccacgttct
    32941 ggttgctgat tgccgggagg gcggcgcagg gcctgggagg ggccatcatg atggctctct
    33001 caatggcgtt tgtcggtgag acagttccaa aggcaaagac cggtagcgcc atgggcctgc
    33061 tcggaaccat gtccgcaatt gggaccgctc tcggcccatc gctcggcggg gttttgattg
    33121 ccgggttcgg ttggcgggcg atcttcctca tcaacctacc cctgggtatc ctgacttttc
    33181 ttctcgcaca tcattacttg cccgttgatc gtctggagcc gaagacggat cgggccggac
    33241 tcgaccatgt gggtacgctg ctgcttgcac tgacgctcgc ggcttatgca ctcgccgtga
    33301 caattgggcg cggcagtttc ggtgccctca acatcgctct cctgttggct gcggcctttg
    33361 gggtcagcct cttcgtactc gctgagagga gagccgcatc gcccttaatc caattggcgt
    33421 ggtttcgcga tccagtgcta agtgccagcc ttgccatgag cgcactcgtc acgaacgtgg
    33481 tgacggcgac gctcgtggtc ggaccattct atctctctcg tgcgctcggg ctcgatgcgg
    33541 ccatggttgg actcgttttg tcagtcggtc cgcttgtcgc cgcactgact ggggtgcccg
    33601 ccggtcgcat ggcggaccgt tttggcgcgc aacgcgtgac catcgtcggg ctcatcggaa
    33661 tggcggctgg ttccttcatt ttatccatga tgtcgaagac atacggtatc cctggctaca
    33721 ttgtccccat cgtgctcatc accggcggat atgggttgtt ccagaccgcc aacaacactg
    33781 ccgtcatgac agatatcagc cctgaccagc gtggcgtgac ttccggaatg cttaatctag
    33841 cgcgcaatct cggacgcatc acgggtgcat cggtaatggg cgctgtgttc gcttatgcat
    33901 cggcaacgat cgacatcaca actgcacatc ccgaggccgt tgccacgggt atgcagatta
    33961 cattcggagt cgcggcgatg gtgattgtcg ttgcgctggc cctcgcgatt ggaactaatc
    34021 gccgcacatt gcacaatcgg gaatttgctc cggaggcaga gttccaggcc gacgggaaag
    34081 ttttagaatg accgctctgc acattaattc aagttgcgca atccattgaa attcatcatg
    34141 ggcgaatggc acatccccat gatgataata ggaaccaggg aagcattaat ggattgcaac
    34201 ccatttgttc tttgcttcct gtccgttgga cagggggaaa atgaaggaag atgtttggtc
    34261 cgttttgaaa aatttattcc cctacccgga caagcatacc gcgctctttg cagatactga
    34321 aggtgtgata aaaccatgcc acgatgatca caagaaaccc aaaattgagg ccggtcgccc
    34381 acagtaaatg catcacaggt aaatccccag tggtgaggac tgcacgcatc ccttcaaaga
    34441 tatgcgcggg agggaccatc caggcaatcg actggagcac aggcggaaga acctcaatgg
    34501 ggtaaaacac gcaagatatg ggttgaaaaa gaaagaccat gccccaggcc agcacttcgg
    34561 cctgctgccc gaagcgcata atgatggaag tggtaagcac cccaatgatc cagccggata
    34621 tcaccaggtt gagaacgaag ggaagtagcg agaaccccat ctggaggata tcgtaggaat
    34681 aaaacaccca ggcaaagagc atcatcaacc cgcctacggc cgtcaccttg agcacactca
    34741 tggtcatggt cgcgacgaga tattctccga ccgttaacgg actggcaaac aggttcatca
    34801 gatttcgagc ccacatttcc tctagaaatg agacggcaat gccttgctgc gcgcgaaaca
    34861 atacatccca gaggatcagt gctccaagaa aaaaggagac ggcgccatgc agcgacatcc
    34921 cgaccttttc cagataaatc gtaatgaatc cccacaccac gagatctaaa aacggccaat
    34981 aaaaaatctc cagcaaacgg gcaaagcttc gcttgtagag atacatatga cgcgataata
    35041 aagcgaggat acgaccgaga ctcatgggcc gttctgttcc ctggccaact ttaaaaagac
    35101 ctcttccaga tctcgttggc cgtagcgttg aatgatttcc ggggcggtgc cttctgccac
    35161 aatcttccct cgttgcagga agatgatccg atcggacatt tcctccatct cccgcatgtt
    35221 gtgcgaggta tagagaatac tcaatccttc agagcgttgg tattctttta aaaacgattt
    35281 aattttattg acaatatcag gatccaaact ggctgtaggc tcgtccagaa acagaacctt
    35341 tggttcggtc atgatggctt tggccaaggt gagccgcgac atttggcctg aagacagttt
    35401 tcttgtgagg cgatgccgga tatcctccat ctccaacttt ttgacaatgt catcaatacg
    35461 ctgttgaata tgcttgagtt catagagccg cgcgatgacc ttcaggtttt cctctaccgt
    35521 taaggaaaac ggcatcgaaa tataggtgga ggaaaaattc acctgttgaa gaatagtttc
    35581 tcgatgtgtg gccaaatcca ggccaaacat gtgaatgctc ccgacggttg gtgtgatcaa
    35641 acccaacaac atatgaatcg tggtggtttt gccagcacca ttcggcccca gcagaccgag
    35701 tatttcacct tttttgatgt cgaaagaaat attatcgacg gccgtaaagc cttgaaaccg
    35761 tttggtgagg tttcgaactt caaccacagg agaagcgttg gaacccgagg caggcattaa
    35821 aacaaaaacc caccgatttt atcaggtaaa tggcaggttt cacagcgatt actcagcact
    35881 tcgccttcgt aacgttgttc cccatcatga caacgaaggc aatcggccat gttggccttc
    35941 cagagcaccg agtcttttgg atgatctggt tcatggggtt ggtcatcgag tttgacatgc
    36001 ccgcgcggga tcacaatggg atacgcttta atcggcgatc catggacgac acgcccgtga
    36061 caggtcgtac acccctcccc tttcccacgc ttctcaaagg cctccatgtg ttcccggtga
    36121 ttcataatca atcccacatc tttcactggt gcgggaagat cacgaacgga gacttctgtc
    36181 acccgcaaga tggctcgatg acaattgatg cacacggagg aagtcacgtg ggatttcaga
    36241 ttgtgcgggt cggtcggggt tccgaagaat gtgatcgcga cgtcttctat tccggcaaag
    36301 actttatcct caatgaaccc ggagatgcct ggacgcacgt ggcaatcaac acaggtgaca
    36361 tctttatgac tggaccgaat ccaggaatcg taggagggtt tgatggtatg acaactcgca
    36421 cagaaggtag gctgattagt cagcgggatt gccgcagcgc ctcctaaggc cagcaccagg
    36481 aatgtggccg caatgagggt caggcctttt cccatggagg ttacgaatcc cttttaaggg
    36541 ggaactgttt cataatcagc tgcgccaggc cctggacgtc gtcccgatca a
//
LOCUS       DAORAQ010000012        36553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_249_length_36553_cov_64.095183, whole genome shotgun sequence.
ACCESSION   DAORAQ010000012 DAORAQ010000000
VERSION     DAORAQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 36553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36553
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249_length_36553_cov_64.095183"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKM72_02300"
     CDS             complement(<1..255)
                     /locus_tag="PKM72_02300"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNP59639.1"
                     /translation="MARKQPTQSSSTSDRKIVKYADQTVETGTGGEVHQTSGKTNPVL
                     TTQQGIPVADDQNSLKIGERGPTALEDFHFREKMFHFDHER"
     gene            531..1097
                     /locus_tag="PKM72_02305"
     CDS             531..1097
                     /locus_tag="PKM72_02305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNP59640.1"
                     /translation="MMMQIGQTIPDGTYQAFHKEQIRSLTLSECQGKWLVLVFYPGDF
                     TFICPTELQELAELYPEFQKLGAEVLSVSTDSVFVHKAWHDTSPAIKNIQFPMVADTT
                     GRLSRDFGTYLEDEGVSLRGSFLIDPDGVLKVGEIHDNSIGRNGRELLRKLQAAVHVR
                     EGKGEVCPASWSPGDKTLKPGLDLVGKI"
     gene            complement(1227..2870)
                     /locus_tag="PKM72_02310"
     CDS             complement(1227..2870)
                     /locus_tag="PKM72_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNP59641.1"
                     /translation="MNTSRTSGWIVGLAAVVLTMGTTSMAPAAGFTAYNDLAWGTGQL
                     ETNITKFTSPNGGSGLPATGLLKDFATGLDTDVTMTVAGGQFVAGANDLQGADPTTGD
                     AFAIFDGKVNGKGVLTYIDDVNNSLVLTFSGLNPGKTYNLTFYAHRNNNEWDRASLVT
                     LSGQDAFTNTSSVATDNPNTGTYPGGVLFTGPTSASTRLPADNDNGYVAQFSGIDPGS
                     DGTVVLTISFDGSVGEQYKGKYGSAVRLIESSGSGGQDLNGDSKADLVFRNTNGNAAV
                     WLLNGAAAPLSIVSLGGISAEWEVEGVGDTDADGNADIIWRNTTTGAVAVWLMNGGTR
                     ISAGFPGTASTAWSIQGVGDLNGDGKADLVFRHTNGNVAVWLLNGTATPSSTGSLGVV
                     SSDYQISGVGDTDADGNADIIWRNTTNGAVAVWLMNGLTRTSVGFPGTASTAWTIQGV
                     GDLNGDGKADLVFRNTNGNAAVWLLNGTATPSSTGSLGAVSPIWQLRQVGDTDADGNA
                     DIIWRNTSSGAVAVWLMNGVTRTSVGFPGTASTAWEIQP"
     gene            complement(3197..4390)
                     /locus_tag="PKM72_02315"
     CDS             complement(3197..4390)
                     /locus_tag="PKM72_02315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HNP59642.1"
                     /translation="MRAVCWNGTHDVRIERVPDPEILNPRDAIVRVTLTAICGSDLHL
                     YDGYIPTMKAGDILGHEFMGEIVEIGAGVQNLRKGDRVVVPFNIACGRCYFCTGKMWS
                     CCDNSNPNAWMAETAYGHSPAGLFGYSHMLGGYAGGQAEYVRVPFADTGPLKIHNGMR
                     DEEVLFLSDIFPTGYQAAENCGIREGDTIAVWGCGPVGQFAIRSALMLGAERVFAIDH
                     FPQRLALARQGHPGQFHVDDRIETINFDENDVYDYLMNATGGRGPDACIDAVGLESHG
                     TTVDALFDRIKVASLLATDRIHVLRQATRAVRKNGTISIPGVYGGFADKFPIGAIFGK
                     GITIRCGQTHVHNYMQPLLERIEKEEIHPSFIISHRYNLDEAPDAYKNFRFHQNEFRK
                     VVLSP"
     gene            complement(4549..5328)
                     /locus_tag="PKM72_02320"
     CDS             complement(4549..5328)
                     /locus_tag="PKM72_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNP59643.1"
                     /translation="MKSTNIGALEQILSLGIGSGLIVHGLGQKTGRGILPLLFGSGLV
                     IHGLTSHSRLYEAIGIDELHGSHIRHPLNRTVHFRGSITINRSQEDLYSFWRDFKNLP
                     RFMQNIVAVQELDERHSHWQARGPFNKNFYWEAEILEERQNELIKWRTIEIHSNLEHE
                     GVLSLRRAVGNRGTILSLDCRWTPPGGIFGAAMAKLLPDDPARQVIEDLRRFRQLMEA
                     GEISRNQRASVDLDHSGIARKAREVGNKPGIVESVGTEQPL"
     gene            complement(5487..5693)
                     /locus_tag="PKM72_02325"
     CDS             complement(5487..5693)
                     /locus_tag="PKM72_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59644.1"
                     /translation="MSTYIRLRMSHDGNHLVVLNFSGEPQTLERDHSFQARVCLSTFL
                     DKEGEEIHGRLDLRGHEGLILSCD"
     gene            5893..6246
                     /locus_tag="PKM72_02330"
     CDS             5893..6246
                     /locus_tag="PKM72_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNP59645.1"
                     /translation="MIHGKKLFLKLKDALEAHSEAEEQVFYTPLQKNEETKQKINHAN
                     KEHEKVTTLLNEIEAMDPQDENWGKRVSKLKEDVQHHIQEEEGEIFKNARGILSQDQA
                     DQMGEEFKQVKEQKV"
     gene            6778..7443
                     /locus_tag="PKM72_02335"
     CDS             6778..7443
                     /locus_tag="PKM72_02335"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNP59646.1"
                     /translation="MKHRVGYGLLVWYLGFFVFMGLAPIDPQSWAFANILPLLFVGVL
                     TMTHRRWPFSSASYVLLTVFLTLHTIGSHYTYAQVPFGLWLEESFELPRNHFDRIVHF
                     CFGLLFALPLHELFSRISGMAAWLRSGMVLITLVGLGGMWEILESWVTRIAHPELGLA
                     YLGAQGDIWDAQKDMAATFYGALASLVIMAGGQKLTGTRLTITRERPVELPHAIDHCD
                     KRQ"
     gene            7412..8074
                     /locus_tag="PKM72_02340"
     CDS             7412..8074
                     /locus_tag="PKM72_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNP59647.1"
                     /translation="MQLTTATSVNNQRPFKNRILLHGLMAWYAVLWIFLAIEPIDRHD
                     WFLENILAIGLVIVLVTTYRWFPLSDLSYIFLTVFMTLHAIGAHYTYSKVPLGYWMQE
                     WWGLERNHFDRIAHFSFGLLLAYPLRELFLRRVNVRGFWAYYLPISGILALSGFFEII
                     ESWVALLVRPELGEAYLGTQGDEWDAQKDMTVAVIGAVLTIMLTYVFSKVVPQKSLPT
                     AP"
     gene            8091..9458
                     /locus_tag="PKM72_02345"
     CDS             8091..9458
                     /locus_tag="PKM72_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59648.1"
                     /translation="MIHLSTAGSIPIHAHGDESKGPHTHERSRHTPIHNWMLALSICL
                     MVISPRVASGEDPDTPLAGQPHQTEIFGQEIHVPPRDRRHVTAINMGMNWIPDGPKRL
                     ELLPFGALFIWHNWEEENRRFRGTISGVFNDIQYQHGLSSTSPWFAAMTFENVIIPFG
                     RYEYVEGQAIKDVDLQWNYFFAGMGFGYRTSVSPGFQDNVVEVSLTYEPGYRWFDRSS
                     KTSQNFTVPQNTYEGRVHARLRVDKFERNLMELLHHGVAFGGDAIYGHRSQWRQWGDP
                     EFETPDNHDDPTFMLASFYAALAGKVPFVPTERHRLVVTMYGGIGKNLDRFSTFRLPG
                     RPTGYEWEALSRPLIPGVAFNELFPRKYAIANLTYRYEALFFLYPYIRGTWSAIERAR
                     FNGDGSIRNQVDMLPAVGGGIVSGAPWGSQIEINYSYNFGIYRDRHGPKLGGNSVVVF
                     WSKLL"
     gene            complement(9543..9746)
                     /locus_tag="PKM72_02350"
     CDS             complement(9543..9746)
                     /locus_tag="PKM72_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59649.1"
                     /translation="MLTIGFILLIIAGLCALIGFGVFTTPILGPARVAFYFLLILSLA
                     TIFYGVVQTEVHVDPQVPTQVRN"
     gene            complement(10213..11964)
                     /locus_tag="PKM72_02355"
     CDS             complement(10213..11964)
                     /locus_tag="PKM72_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNP59650.1"
                     /translation="MTKTAADVLVEKLHNWGVEIIFGMPGDGINGIMEALRQRQDHIR
                     FFQVRHEEAAALMACGYAKYTGKLGVCLATSGPGGLHLLNGLYDAKMEKQPVLAITGH
                     HFHDLIDTHAQQDVNLTRAFEDVTVYNTRVMSATHLENVVDLACRTALGYRGVAHINF
                     PVDLQESKADKGYSQRNIPHHSMEVFSRSARLPNESDLYEAAEVLNVGKRIAIMAGAG
                     ALEAIDELEQLAEILGAPIIKPLLGKASVPDDSPYTTGGIGLLGTAPSQEALEDCDTL
                     FLIGTSFPYLEFYPKPGQARAVQIDLDPARIGLRYPVEVGLVGDCRKTLQQLLPMLHH
                     NKHRKFLERAQTNMKKWRELMEARGTRKDKPMKPQVVAWELGKRLSDHAIVSCDSGTI
                     TTWWARQIPAKRGQKHTVSGTLASMGCGLPYAIAAQIAFPDRQCIAFVGDGGFSMLMA
                     EFATCVKYRLPVKVVVIKNNTLGQIKWEQMVFLGNPEYGCELQPIDFALIAQACGGTG
                     LTIDDPADCGSIIEQALQTPGPVLVQAIVDPFEPPMPANITTSQALKFAESLARGEPN
                     RLKIAMTALSDRVRELV"
     gene            complement(12033..12413)
                     /locus_tag="PKM72_02360"
     CDS             complement(12033..12413)
                     /locus_tag="PKM72_02360"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HNP59651.1"
                     /translation="MTQAMGATRWAIPEGYIPANSTGPEPQMTSHETACLLNISNKPA
                     HVKITIFYSDREPIGPYNITVPAQRTRHVRFNDLEDPEPIPRDTDFASLIESDVPIVV
                     QHTRLDSRQAENALLSTIAYTDNS"
     gene            complement(12410..12589)
                     /locus_tag="PKM72_02365"
     CDS             complement(12410..12589)
                     /locus_tag="PKM72_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59652.1"
                     /translation="MNEPMNISDSQGSHLSGNINPSLPSYLLEAALDREWERRHRQGQ
                     VGKAPRTPAEKEIGS"
     gene            complement(12658..13815)
                     /locus_tag="PKM72_02370"
     CDS             complement(12658..13815)
                     /locus_tag="PKM72_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNP59653.1"
                     /translation="MAPKKTSTKKRSRTQTKPSNPIRYAVVGLGYIAQVAVLPAFAHA
                     KKNSELAAFISDDQKKLKQLGKQYGIRGLYSYDQYEECLEREKIDAVYIALPNHLHCE
                     YTTRAAKAGVHVLCEKPMATSVKECETMIRTADHHGIKLMVAYRLHFEEANLRAIQIV
                     QSGKIGNPRYFQSAFSLPVKDKNNIRLREETGGGTLWDIGIYCLNAARYLFQAEPLSV
                     YATNAGQGNGKFEEVEEMCAVTLRFPEDRLASFICSFGAADVSEFRIVGTKGQLRVTS
                     AYEYVEPITHYLTINGKEKKQTFSKRDQFAPQLLKFSDCILNRTIPEPAGDEGLHDVR
                     IIDALYRSAQKGQPVSLKELNRKRRPTMRQNLRRPPVKKPQLVHAQSPSGS"
     gene            complement(14062..14868)
                     /locus_tag="PKM72_02375"
     CDS             complement(14062..14868)
                     /locus_tag="PKM72_02375"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNP59654.1"
                     /translation="MNTRRRTSGTLGMALAFALIGGGMSLALSESGGSTHPSNELLLN
                     ANTLIGNTVIDKAGKELGTVKDLLIDQKTGQISYIVLSYGGTFGGTLGIGSENYSIPW
                     AEVKLTKQDDKMMVQVAEAPIGEKAPAKNHPSVHNQQAAGIHTRDFNPATVETVDGTV
                     ENVNNDLFESGVATTDSLIVLDVKTSSGTERVRVAPDNYLKAQGVEVTEGDKVEVTGS
                     RIMKEGESVIVASKIVLKRNGKVLTVRKIDGSPAWNMDGSVHTKSKTGHD"
     gene            15562..15948
                     /locus_tag="PKM72_02380"
     CDS             15562..15948
                     /locus_tag="PKM72_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59655.1"
                     /translation="MSRTRIEMGVVMLGFSITLLLNAQGFPQGLPESSAQQLQISADE
                     AQSHQPNQFGEKKDLQKPQPVIPPNNDAEMVIKPDVPTNPEAVITPPVVDPEMAVDPT
                     TRQPMTDEKLEELTPEELEENARGEK"
     gene            complement(16159..17019)
                     /locus_tag="PKM72_02385"
     CDS             complement(16159..17019)
                     /locus_tag="PKM72_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNP59656.1"
                     /translation="MTWREFQRLQLVSRLNDRFLSYVEAGTGPPVILIHGIPVWGYLW
                     HGIFSELARTRRVLVPDLLGFGFSDKADCFDRSIACQAHMIASWMTQLGIPRATIIGH
                     DIGGGVALRLATLFPDRVQTLIVMNTVCYDSWPIELMLQFGHPEAHRKLSASLAVRLL
                     RQALKGGFASSVNREVLDGLLAPYTTETGKLSLIRNAAALNTNLTTEITHLLSRIRIP
                     TLVVWGQNDRFQPIHYGERLVKDIQGSRLIRVKDARHFVMLDQPEIVTRHLLEFFERT
                     DEMSFKDTRV"
     gene            complement(17016..17912)
                     /locus_tag="PKM72_02390"
     CDS             complement(17016..17912)
                     /locus_tag="PKM72_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     acid halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="halocarboxylic acid dehydrogenase DehI family
                     protein"
                     /protein_id="HNP59657.1"
                     /translation="MKNHPIPEEEAEKELDSIFHEIKQVFRVSGVNLNFRTWAAYPTF
                     FPVLWESIRPVAETRVFEDSSDQLRALAARLAEKLPRLEVASSVRLGESQVFHIQAAL
                     ELYHYINPKLLLITSAVEYACHHPTMTAFQSRQQDHELITRGIPRHMYPMEMIEETPD
                     NATLRKTFRDLQRTLGLTRINSDYRTLALWPEYFLSVWHRLKSVIHQPRYMEAALALQ
                     EAAQQMAGEVIPRLTLSEDQLRILGRKRQSFLETTSDFTHLLPPLILNIVLMSLDWRS
                     REDLEMSPFPVGVPSPIMKSPT"
     gene            18118..18606
                     /locus_tag="PKM72_02395"
     CDS             18118..18606
                     /locus_tag="PKM72_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59658.1"
                     /translation="MMTSPTTTRVEENTDADINEEIYNRTKNNVLLYASAGPSAIDRR
                     LRELEVEWDIERILEANAASFSLMGLALGAFIDRRWFIFPAAVAGFLLQHAIQGWCPP
                     VPLFRRMGIRTAHEIQQERHALKALRGDYRDVAELKAEVNGADALQVTRAFEAAGNNS
                     ST"
     gene            18648..20042
                     /locus_tag="PKM72_02400"
     CDS             18648..20042
                     /locus_tag="PKM72_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP59659.1"
                     /translation="MIIKQFHVESLGHASYLLGSEETGEAFVVDIQRDVEPYLKESRT
                     QGMTIAYALETHQHNDYVTGIRELAERIPLQCLAGRHAKLGYDARPMADGERLTMGEI
                     DFQILHTPGHTPEHISVLVTDRSRGKEPVLLLSGGALLVGDVARPDLLGGTEEAAHHA
                     EVMCKMLQEKILPLPDYVEVYPTHVSGSLCGGNIGSRLSTTIGYERRMNPLLADLSNK
                     DHFVKHCLDLKHLPGVPPYWPRMRKMNTEGPPLLGTMPEPPALTPDQFCRQQEEGCLV
                     VDCRQAEGFTTHIPGALNVGLGSSFATWAGTVIPADTRVLLVLDHPADLWEVSWQLLR
                     IGYPTPAGWLAGGMFAWRTAAKPLHQLTQWSVWDLQAHLEKDSDLEVLDVRQPGEWAA
                     GHIKNARFVTGSELPQRVQEIRKNLQVAVICGSGYRSSVAASYLQHHGYPNIVNVIGG
                     MSAWKKANLPIIKD"
     gene            complement(20584..20928)
                     /locus_tag="PKM72_02405"
     CDS             complement(20584..20928)
                     /locus_tag="PKM72_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59660.1"
                     /translation="MTKLLTVMGMSAFLVFSGVSVFAGQAGMNDQQRQAGDSPSMQGE
                     QNQGTIESKSQSNRNTEPTEDQQREARPGGVGEEGSTFGSPSTFPPGDTSQGYTGEDK
                     KAEEREKRQQGM"
     gene            complement(21046..22659)
                     /locus_tag="PKM72_02410"
     CDS             complement(21046..22659)
                     /locus_tag="PKM72_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59661.1"
                     /translation="MKLRSHMVKTIFLAGIPLLTFPLLAESGMSREDRSQRGDRGHSN
                     AIEQIIEGEISKKNGEKITLRTSDGERITFRITDQTNQLCPQGTQTSQSSSMSGESQK
                     NQHSAMDQGNSTGKSSAQSTSGKNQMAQNSPGGDSSTSAQTSSSNLLENPSGRASGQS
                     SPGMSSDSQPSQQQGFRFGECNFQKGEFVKAKLGENNTATFVRSMGNSEDYYGTAGVE
                     GEYFVLPAGQLGGLDISDKDAHYSVNSNDGKEIGSIYRVMTNNNGDIAYAIIRKPDGQ
                     LISVPWQALEGSGNKSFKMKVTQNQLGNLPILNDGENVGAHVQKNWDLSEQERQNLAK
                     WNERLDERDQVRDRYYEDTPTFRNNREGYDRYDQDRSRMSRSGDDRQRHYQDQNAEDY
                     QTKRNNFSPTEFPPHDRAFQYEGADQQNSTTRNRQGSYSDDYRDRGNEQYSRSEGRNY
                     NNSGDREYRNQSRSNRPNYDDLQGDRQRSMYSQNREGNYRDMEQLSDRYNTTAPRRDY
                     DSQRQNDDQYQSRRSNMKEYDDRPEGYRP"
     gene            22863..23222
                     /locus_tag="PKM72_02415"
     CDS             22863..23222
                     /locus_tag="PKM72_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNP59662.1"
                     /translation="MTTKIRQVLSSSSITGDTVQNMDREDLGEIKDLMIDLSEGRIAY
                     AVLSFGGFLGMGDKLFAIPWEALSVVQEEKVFLLNVPKEKLEQAPGFDKDNWPDMADV
                     TWGQSIHEYYGYNPYWG"
     gene            23575..23916
                     /locus_tag="PKM72_02420"
     CDS             23575..23916
                     /locus_tag="PKM72_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNP59663.1"
                     /translation="MADTSVKKVQADHSPKGSMGQKYLASGVGLAMRLWEELPVGYTH
                     ESSTRDYETIGYVIQGRAELQLEGQTLLLNAGECWVVPKGASHSYHILETFTAVEATH
                     PPAHIHGRDEV"
     gene            complement(23964..24836)
                     /locus_tag="PKM72_02425"
     CDS             complement(23964..24836)
                     /locus_tag="PKM72_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNP59664.1"
                     /translation="MPNPFSVIRFQQVKLFCVLCMLTGMAMGCETVPYTDRSQLVVVP
                     QSQAAEMGEQAFQQILSDPKVQQSQSAEEQEAVNRVADRIIQAAKESKFAEDAKAFQW
                     EVTVIKDDQTKNAFALPGGKIAVYTGIFPVAKNDAGLAAILGHEVVHALARHGSERMS
                     QEVLSQAGLSAAAVGLGASGAGPVVGQATMAALGLGAKVGVLLPYSRTHESEADTIGI
                     LLAAKAGYDPQEAVRVWQRMQEGSQGTPSEFLSTHPSHETRITRLKEAMPEALEIYQK
                     VQHAPDTPLPPVGG"
     gene            complement(25113..26054)
                     /locus_tag="PKM72_02430"
     CDS             complement(25113..26054)
                     /locus_tag="PKM72_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HNP59665.1"
                     /translation="MFKAFRNLSKQGILGLNKRNAVYTLGFNQRRFYPFVDDKAQTKT
                     LCSQAGMAVPELYGKIEIERQIRDLPKLLNTHTEFVIKPAHGSGGDGIVVIRGRVKDK
                     YRKANGIILSLDTLQHHISNVLSGMYSLGGAADTALIEYCVNFDPIFESISYQGVPDI
                     RIIVFLGVPVMSMVRLPTRMSDGKANLHQGALGVGVNMATGTTKTAVLLNEIVSEHPD
                     TNNSVTGLVIPNWKDLLTLASQCYDIFKLGYLGVDIVLDRDQGPLLLEVNARPGLNIQ
                     IANCSGLLPRLHLVEQSARDLTSVQDRIEFAIRQFQS"
     gene            complement(26056..27516)
                     /locus_tag="PKM72_02435"
     CDS             complement(26056..27516)
                     /locus_tag="PKM72_02435"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UUP1 family membrane protein"
                     /protein_id="HNP59666.1"
                     /translation="MLGFPLNPESTSEVWNVESRISFQATDGPVQVSMFTPKSILPYI
                     ITTESFIGQEYGVTEKVEGPNRLVTWSKRQAEGVQILYYRGIIRQMKNWETEFQQFTD
                     ALQQPQDSQGNLPTLEGAFLQAAIALLAEVKKQTADTTSLTLSLIRRFTKINPDSYEA
                     LLLSQQPAEQAIAQIITLNGTPARVVHGIELSREARTVNLKEWIEVFDEDTWVPFDLE
                     EVKTGIPDNYLSWWRGDAPLFHIDGGKELEFRLSASPNQEKAIQAAVARGMVMNPAWL
                     KFSLFSLPVEKQIVYHILILIPVGAFLLVLLRNVVGLKTFGTFMPILIALAFRETQLL
                     WGITLFSLLIALGLAVRFYLEHLKLLLVPRLAAVLIVVVLMMGVVSVLSHHLGIERGL
                     SVALFPMVIVTMTIERMSIVWDERGPGEALQQATGSLAVASLTYLLMSLETMDYVFFT
                     FPELLLILLACTIALGRYTGYRLLEVRRFQELLKSS"
     gene            complement(27591..28118)
                     /locus_tag="PKM72_02440"
     CDS             complement(27591..28118)
                     /locus_tag="PKM72_02440"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HNP59667.1"
                     /translation="MTELNGRLTLAFMLTFGGALFQPPTPLMAKDHPIRAGWVEEVIL
                     IPDSIRLEAKLDTGAKSASLHAENLSTFSKDNERWVRFDITAQDGKHTTLERKVHRTV
                     KIKQHERESSERPVILLDLCLGTHIEQTEVNLTDRSNYKYPLLIGRMFLSGHFIVDSS
                     TTHLLKPDCSSQSKK"
     gene            complement(28373..28732)
                     /locus_tag="PKM72_02445"
     CDS             complement(28373..28732)
                     /locus_tag="PKM72_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59668.1"
                     /translation="MEDNPDHREILKTRLTSLGYVCQETDDGHEGLCALSYSRFDFVM
                     CDYQMPEMNGLQFIHTVRTTSGISTIPIIFMTAHSDPFFIDHAVSVGATATLVKPFSL
                     ENLKTALDRIWTMKRNS"
     gene            29423..30103
                     /locus_tag="PKM72_02450"
     CDS             29423..30103
                     /locus_tag="PKM72_02450"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNP59669.1"
                     /translation="MLRDLDKINNCVLQGTDGIIGSVADVYVNDQTWKIHYLAADTGN
                     WLQGRHVLISTQSFVHPHEDIGSFRALVNKAQVEEAPKFEIEKPISWEKEREICNYYR
                     WSTYYPIDERELSVFPGLLTSAKGLKDFSLQATDGEIGKIINFLIDDANWTVRYLVVD
                     TSKWLAGRKVLISPMWNKSIDWLERMMVVDLNQSQIEQSPEYDPAAPIERVYEINLYK
                     HYGKPHYW"
     gene            30326..31786
                     /locus_tag="PKM72_02455"
     CDS             30326..31786
                     /locus_tag="PKM72_02455"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNP59670.1"
                     /translation="MYEHLIIFGATGDVTKRFVFPALAHVFANRGLPREHRITGVGRR
                     DWTTPHFQEHVSELLDNHESSPPPRSREGFLESLDYARVQDISDPRQIQAIFSQQAGS
                     TLLYLGLPPQMFPSVLETFRHVTLPPESRVIIEKPFGLSYAQSMELNGLLHQKFPEGM
                     VFRIDHFLGKPVVDTILGLRFANPVFTPLWNRHHIKKIEVIWDETLALEGRADYYDHA
                     GALKDMIQNHLLQLMAVLALGPLESMWSPEFRDKRVELFKAIRKPSKPEIRAQTVRAR
                     YESGEIGGVRIPGYTENRGVVSERNTETFAQVTLWVDNERWQGVPFVLRSGKALGRDR
                     QEILVHFKGGQRSTESLLSEEGGNILRINLGSEKVNLGIFTIHEPGGITPEPMRVDDP
                     VSPEHLSPYERLFLEAMSDKLRLFVRNDEVEEMWSIMQPIADAWAENLVPLQIYRAGS
                     YGPSMGDQGDVGESMESKMSRTKEESPARDPSSSYL"
     gene            complement(31823..32251)
                     /locus_tag="PKM72_02460"
     CDS             complement(31823..32251)
                     /locus_tag="PKM72_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNP59671.1"
                     /translation="MDGLIDLNWHQVWSHLYHLLIAFLLAVPLGWDREHESRGAGLRT
                     FPLVAVGSCAYMLTGINVLNSTDAEARVIYGILTGIGFIGGGAILKERGNVAGTATAA
                     SIWNTGAIGMAVAWDRYEIALVLSVLNFLTFRFMPLLKNK"
     gene            complement(32315..33205)
                     /locus_tag="PKM72_02465"
     CDS             complement(32315..33205)
                     /locus_tag="PKM72_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNP59672.1"
                     /translation="MEPLNIILNSLREMGSQALAHLPNVISAIVILALTWLSTKALET
                     LLTRLLSRFGIRESLLELLQKLVYIGIWIVGILIAAIIIFPSLTPGKILTGLGLGSIA
                     IGFAFKDIFENFLAGILILLREPFQLGDVIECEDLEGFVEEITIRDTHIRQTDGQRVV
                     IPNSVLFKNPVIVRTDRDLRRVTIMCGIAYGEDVNAGRDVIRRALEGLDTVNQEKDIQ
                     VFAQGFGASSIDFEVTWWTGSRPVDIRKSKDKVVAAVKQSLDSAGIEIPFPYRTLTFK
                     EPLETLITRHRKEQDVPHDT"
     gene            complement(33223..34401)
                     /locus_tag="PKM72_02470"
     CDS             complement(33223..34401)
                     /locus_tag="PKM72_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNP59673.1"
                     /translation="MPMPTPSTTRRKLSRNLHLWEVVAIRELCLLGVVVWFLWLCLQL
                     QAILVPVLIGLGLAYLADPTLDYVEQAWRLPRWVAVAFMAMIMCLLFSGLVLWIGPLL
                     VDQLRHFFEKVPTYLSILANRYEVDLEDISRRLYEVSTNIKSNPFATLKTFFSGTGQV
                     FYFTNIIIGTLGSFLFSLSLIVIYFFFFAWSFPSIQKTFWRLLESQGDPRWEKLLTEM
                     DVAIGEFFRGRLLIALIMSVMFGGGWYLVGMPYWVLLGFCAGVLSLIPYAATLMWPIA
                     LLLKYLDMSMGPSSIDDYWMQVLVWPSAVYLGVQFIEGWVLTPWIQSQSTDLSAATIL
                     LVVLIGGALGGVLGLILAIPFAACLKILAKELIIPRLQSGDITEVHGKIITPQSSGLT
                     "
     gene            35243..35422
                     /locus_tag="PKM72_02475"
     CDS             35243..35422
                     /locus_tag="PKM72_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNP59674.1"
                     /translation="MTILKWAAIFFVIALIAGGFGFSGIAEGAAGIAQVLFYIFLAIC
                     VVMVLIGVFVAKKIT"
     gene            35542..35835
                     /locus_tag="PKM72_02480"
     CDS             35542..35835
                     /locus_tag="PKM72_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59675.1"
                     /translation="MTNHEKAKLIAPWINPAERVTVDFKDEIGLNAEVFGCTENVVHL
                     LFQEAFPHIKEQITIPLREVEVGEDHGRYTRDPDTPLQWRLRLQVNRNRPEGM"
BASE COUNT         9203 a         8820 c         9174 g         9356 t
ORIGIN      
        1 ccgttcatgg tcaaaatgaa acatcttctc ccgaaagtga aagtcttcca atgcggttgg
       61 accccgctct ccgattttca gcgagttttg gtcatccgcg acgggaatgc cctgctgagt
      121 ggttaatacc ggattggttt ttccgcttgt ctggtgcact tcccctcctg taccggtctc
      181 gacagtctgg tctgcgtatt tcacgatttt acggtcggaa gtagaagaag attgagtggg
      241 ttgctttctg gccatgcgaa tcgctccttt ttattgatga tttcgtaatg aataggtgag
      301 taaacatatg atcaacttat gacgctaacc tacctatcat ataggaccaa ttgaagaggc
      361 tatactgtaa gaaaccctgg tgagaaattt cagaaagttt tttgcattcc ggggattaga
      421 tttgtttaag taacttgaga attggagttc agatacccac gcccgaatat ctgctctgca
      481 acccttgtgt taaacatttc aacccgtttc aataaaggag gtcgaaaagc atgatgatgc
      541 aaattggaca gacgatcccg gatggcacat accaggcttt ccacaaagag caaattcgtt
      601 cactgactct gtcggaatgt cagggaaagt ggttggtgct ggtgttttat ccgggagatt
      661 tcacgtttat ttgtccaact gaattgcaag aactggcaga gttgtatccg gaatttcaaa
      721 aactgggggc tgaggttctt agcgtcagca ccgattcggt ctttgtgcat aaagcctggc
      781 acgatacctc gcctgccata aaaaatattc agtttcccat ggtagctgat acgacgggcc
      841 gcctaagccg ggatttcgga acctatttgg aggatgaagg ggtatcgtta cgcgggagct
      901 ttctcatcga tccggacggt gtgctcaaag ttggagaaat tcacgataat agcattggac
      961 ggaatggtcg ggagctctta cgaaaactcc aggctgccgt ccatgttaga gaaggaaaag
     1021 gcgaagtatg tcccgccagc tggtcccccg gcgataagac cttgaagcca gggttggatc
     1081 tggttgggaa aatttaatgt aaagaccaag gtcgatcaac catttcatct ggagtaggga
     1141 tctgcgtcat ccagccaggt gactctcgct tgtgaaagaa gcgagagtca cctatagcag
     1201 gccctttatt cggcaggtgc atcaagctaa ggttgaattt cccaagccgt cgacgccgtg
     1261 ccggggaagc ccacggaagt cctggtcacc ccgttcatca accacaccgc caccgcgccg
     1321 ctcgaggtat tgcgccagat aatatccgcg ttgccatcgg catccgtatc gcctacttgt
     1381 ctcaactgcc agatcgggct caccgcacct aaggagccgg tagaggaggg cgttgcggtc
     1441 ccattcagca accagaccgc cgcgttgcca ttcgtgtttc taaagaccag atcggccttg
     1501 ccatctccat tcaggtcacc gactccctgg atggtccacg ctgtcgacgc cgtgccggga
     1561 aagcccacag aggtccgggt caacccattc atcaaccaca ccgccaccgc gccgttcgtg
     1621 gtattgcgcc aaatgatatc cgcattgccg tcggcatcgg tatcacccac ccctgaaatc
     1681 tgatagtcgg agctcaccac acctaaggat ccggtagaag agggcgttgc ggtcccattc
     1741 agcaaccaga ccgccacgtt gccattcgtg tgtctaaaga ccaggtcggc cttgccgtcc
     1801 ccattgagat ccccgactcc ctgaatagac catgcggtcg acgctgtgcc gggaaagccc
     1861 gccgaaatcc gggttcctcc gttcatcaac cacaccgcca ccgcgcccgt cgtggtattg
     1921 cgccagatga tgtctgcgtt gccatcggca tcggtatcgc ctactccctc tacttcccat
     1981 tcggcactta tccctccgag agagacgatg gacaggggcg ctgctgcccc attcaacagc
     2041 cacactgcag cattaccatt cgtattccta aaaacaaggt cagctttgct atctccattc
     2101 aaatcctgtc cacctgaacc tgatgactca atcagccgaa ccgcgctgcc atactttccc
     2161 ttatattgct cacctacgct tccatcaaaa ctgatagtca gcaccaccgt cccatcgcta
     2221 ccgggatcaa tgccactaaa ctgggccaca tagccattat cgttgtccgc tggcaatctt
     2281 gtagaagcac ttgtcggccc cgtaaacagc acacccccag gatacgttcc cgtattagga
     2341 ttatcagtcg caacggaact cgtattcgta aaggcgtcct gcccggaaag agttaccagg
     2401 gaagctcgat cccactcgtt attgtttcga tgagcataga aggtcagatt atacgttttg
     2461 ccagggttta gcccgctaaa tgtcaacaca agggaattat tcacatcatc gatataggta
     2521 agcacgccct tcccgttgac cttcccatca aagatcgcaa aggcatcacc ggtagtcggg
     2581 tctgcccctt gcagatcatt ggccccggca acaaattgac cgccggccac ggtcatggtc
     2641 acgtccgtat ccaggccggt agcaaaatct ttcagtaggc cggtggcagg aaggccagag
     2701 cccccattcg gagaagtgaa cttcgtgata ttcgtttcca gttgcccagt cccccaagcc
     2761 agatcattat aggcggtaaa accagctgcc ggagccatag aggtcgtccc catggtcaag
     2821 accaccgccg ctaggcccac tatccacccg ctagtccgag acgtattcat tttcataaat
     2881 ccccctttct ttttccgcct tgagttaaac catttttggg atgagcgctg tccatttcac
     2941 gatacctcct ttaaaagtct atgccggcac atggttgagg gggatctagc aaagagtcag
     3001 gccgcagcct attgagctca cgcggatgtg ccgcttgtgc gactggccgg tggtcttcca
     3061 ggagagagag aagaaatagg cgggtggaaa atttcctcta tccctcataa tcttcttcat
     3121 ctgttattgt aaggagacat tcagggttgt aatgaccgaa ccttcgttta gtattccgat
     3181 tatattccgg caagcttcaa ggggaaagaa ccactttcct gaactcattt tggtgaaacc
     3241 gaaaattttt ataggcgtca ggagcttcat caaggttata gcggtgtgag atgataaaag
     3301 acggatgaat ctcttccttt tcgatgcgtt caagcaatgg ctgcatataa ttatgcacat
     3361 gagtctgacc gcaacgtatg gttatgcctt taccaaagat ggcgccaata ggaaatttat
     3421 cggcaaaacc cccatatacg ccgggtatgg agatcgtgcc gtttttgcga acggctcgtg
     3481 tcgcctggcg aaggacatga atgcgatcgg tcgcgagaag acttgccacc ttgatccggt
     3541 cgaaaagggc gtctaccgtt gtgccgtgag attcaagtcc tacggcgtca atgcaggcat
     3601 cagggcctcg gccaccggtc gcattcataa ggtagtcata gacatcattc tcatcaaaat
     3661 taatagtttc tattcgatca tccacatgaa attgtcctgg gtggccctgg cgcgccaggg
     3721 ccagacgttg ggggaaatga tcaatggcaa ataccctttc cgctcctagc atcagggcgc
     3781 ttcggatggc gaattgcccc acgggtccac aaccccagac ggcaatggtg tcgccttccc
     3841 gaatcccgca gttttccgct gcctgatatc ccgtgggaaa gatatcggag aggaacaaca
     3901 cttcctcatc cctcatacca ttatgaattt tgagcggtcc cgtgtccgca aagggcactc
     3961 ggacatattc ggcttgtcct cctgcatacc cgccgagcat gtgggaatat ccgaacagtc
     4021 cggcagggga atgtccatag gccgtctctg ccatccatgc gttggggttg gagttgtcgc
     4081 agcacgacca catcttgccg gtacaaaaat aacaccttcc acatgcgatg ttaaatggga
     4141 cgacgacccg atcgcctttt cgtaggtttt gtacgccggc tccgatttcg acaatttctc
     4201 ccataaactc atgaccgaga atgtcgccgg ccttcatggt cggaatgtat ccgtcataca
     4261 ggtggaggtc tgagccgcag atagccgtaa gagtgacgcg tacgatcgca tcacggggat
     4321 tgagaatttc cgggtcggga acgcgttcga tccggacgtc atgtgttcca ttccaacaga
     4381 ctgctcgcat gtaaagattc cttcccgccc tcgacaggga ttgacgaatt gtttagaaga
     4441 gcaaacgcct acacaatcga ctttttccgg atccggaatt gcctttttgt tcaaaagtga
     4501 agtcgacaaa aaaaaggttt tatgccccca gagcctcagc atggacaact agaggggttg
     4561 ttccgtaccc acggactcga cgatgcccgg tttgttgccg acttcccgtg ccttccgcgc
     4621 gattccggaa tggtcaagat cgactgaagc ccgctggttt cgggaaattt ctccggcctc
     4681 cattaattga cgaaagcgtc gaagatcttc aatcacctga cgtgcagggt catcgggaag
     4741 gagctttgcc attgctgcgc cgaatattcc tcccggcgga gtccatcgac aatccaggga
     4801 gagaatggtt ccgcggtttc cgacagcgcg tcgcagggac aagactccct cgtgttcaag
     4861 gttggaatgt atttcaattg tccgccattt gatgagttcg ttttgacgtt cttcaagaat
     4921 ttcggcttcc caataaaaat ttttattgaa gggaccacgt gcctgccaat gggaatgtct
     4981 ctcgtcgagt tcctggaccg caacgatatt ttgcatgaac cgtggtaaat ttttaaaatc
     5041 ccgccaaaac gaatagaggt cctcctgtga tcgattgatc gtaatggacc ctcggaaatg
     5101 gacggttcta ttcaggggat gccgtatatg agatccatgc aattcgtcaa ttccaatagc
     5161 ctcatacagg cggctatggc ttgtaaggcc atggatgact aacccgctcc cgaataacag
     5221 gggtaaaata cctcggccgg ttttttgccc taggccatga acaattaaac cactgccgat
     5281 tcctaaggaa aggatttgct ccagagctcc gatattcgtg cttttcatat gacatctcca
     5341 atacaaatta ttccagccca gagaatcggg tgattggccc tcacgccatc gggatcacca
     5401 tacgaaataa cttataccct ctcgtgctcc acggttactg gtaacatccc tggaaataaa
     5461 ccagaaacag accaatgaag gttttttcaa tcgcatgaga gaatcagccc ttcatgtccg
     5521 cgcagatcga gacggccgtg gatttcttcc ccttccttgt ccagaaacgt ggaaagacag
     5581 acgcgggcct gaaacgaatg gtcccgctca agtgtttgcg gttcaccact gaagttgagc
     5641 acgaccagat gattaccgtc gtgactcatt cgcagccgga tgtatgtgga catttatgcg
     5701 agcctcttta ccaattcgtc catacctctc atcaaccgct ccgtggtgta ctacggaaca
     5761 tcatattgat gttccggtag aagaacttaa cattcaatag gggggcttta atggatattt
     5821 atcaggcgct caaggaagat catcaagaag ctaagcaact cttcagccaa atcgaagccg
     5881 cggggaagaa cagtgattca cgggaaaaaa ctgtttctca aacttaagga tgccttggag
     5941 gcgcatagcg aagcggaaga gcaagtcttc tatactcccc ttcaaaaaaa tgaggagacc
     6001 aaacaaaaaa tcaaccatgc caacaaggag cacgaaaaag tcaccacgtt gctgaatgaa
     6061 atcgaggcta tggatcccca ggatgagaat tgggggaaga gagtcagtaa actgaaagaa
     6121 gatgtgcaac atcatattca ggaggaagaa ggcgaaattt ttaagaacgc tcggggtatc
     6181 ctgtcacagg atcaggccga ccagatgggg gaagaattca agcaggtcaa ggaacagaag
     6241 gtctaagaca aaggatatgg atttgcctcg gagacgaaca tctatgatcg gaatggagga
     6301 agacgatggc ggataattgg ggattatgta agaggtgcca atggtggcaa attgaaccat
     6361 ctgcaaatat cgaagatgcg acaatgggac tgtgtattga tgaaaactta caaccctttc
     6421 gattgttggt ctccggcaat agtggatgca atagattcat gaaaggcacc cctgcgaaag
     6481 cggaagggtc ctcttccagt cctccgactg ccgagccaac ccgttaggtg aacggggttt
     6541 gacctgccag tgagatgggg gaggctggag gctgtctctc acctgagtcc gtcgggtggg
     6601 atgcatcaaa gggaagaatg gcgaatcggc tttgtcaagc caggtactca tcaatgggtt
     6661 caagcctcag tctatgtacg aaatttttcc cgttgacttg tttttcaggg gttcatggtg
     6721 accaatatat attacccatg taatggctta ttccgaacgc tctgaggagg agtttatttg
     6781 aagcatcgag tagggtacgg attattggtc tggtatttgg ggtttttcgt gttcatgggg
     6841 ttggctccca ttgaccctca aagttgggcc tttgcgaata tcctcccgct tctcttcgtc
     6901 ggtgtgttaa ccatgacaca tcgccgatgg ccattttcaa gcgcttcata tgtcctcctc
     6961 acggtgttcc tcaccttgca tacgattgga tcacactaca cctatgctca ggtgccgttc
     7021 ggactttggc tggaagagtc ctttgaattg cccagaaacc atttcgatcg catcgtccat
     7081 ttttgtttcg gcctactatt tgccctgcct ttgcatgaac tcttctcccg gatttccggt
     7141 atggctgcat ggctccgctc cggcatggtc ctgatcaccc tggtggggct gggcggtatg
     7201 tgggaaattc tcgaatcatg ggtgaccaga atcgcccacc ccgaattagg tctggcctat
     7261 ctgggcgcac agggagatat ctgggatgcg cagaaggata tggccgcgac attctatgga
     7321 gcattagcct ctctagtgat catggctggt gggcagaaac taacgggaac ccgtttgacc
     7381 ataacccggg aaaggccggt ggaacttccg catgcaattg accactgcga caagcgtcaa
     7441 taatcagagg ccgtttaaga accggattct ccttcacggg ttaatggcct ggtatgcggt
     7501 gctttggata tttctcgcca tagaaccgat cgaccgccat gattggtttc tcgaaaatat
     7561 attggcgatt ggattggtca ttgtgcttgt cacgacgtat cgatggtttc ccttatcgga
     7621 tctttcctat atttttctca cggtgtttat gaccctccat gcgatcggag cccattatac
     7681 ctattcaaaa gttcctcttg gatattggat gcaggagtgg tggggattag agcgcaatca
     7741 ttttgaccgg atcgcgcatt tttcattcgg tctgctcctc gcctatcctt taagggagct
     7801 atttcttcgc agggtcaacg tgcgtggatt ctgggcgtat tatctcccca ttagcggaat
     7861 attggccctc agtggattct tcgaaattat cgaatcctgg gttgctctcc tcgttcgtcc
     7921 ggaattgggg gaggcctatc taggtactca aggagacgaa tgggatgcac aaaaggatat
     7981 gaccgtggcg gtcatcgggg ccgttttaac aattatgctg acgtatgtct tctccaaagt
     8041 cgtgccacaa aaatcccttc cgactgctcc ttagaatttc cgatttccct atgattcacc
     8101 tgtccactgc gggctcgatt ccgatccacg ctcacggaga tgaatccaag ggaccacaca
     8161 cgcacgaacg tagccgccac acccctatcc ataattggat gttggccttg tcgatatgcc
     8221 tgatggtgat ttcccctcgg gtcgcttcgg gggaagatcc cgacacgccg cttgcaggac
     8281 aacctcatca gacggaaatt tttggacagg aaattcatgt ccctccacgg gatcgccggc
     8341 atgtgacggc gatcaatatg gggatgaatt ggattccgga cggacccaaa agattggagc
     8401 tcctcccatt tggagcgttg tttatttggc acaattggga ggaggaaaac cggcggtttc
     8461 gggggacaat ttcaggcgtt ttcaatgaca ttcagtacca gcatggtctg tcatcgacca
     8521 gcccatggtt tgccgccatg acctttgaaa atgtgatcat tccattcggc cgatacgagt
     8581 atgtagaagg acaggctatc aaggatgtgg acctgcaatg gaattatttc tttgccggaa
     8641 tgggtttcgg gtatcgcacg tcagtttctc ccgggtttca ggataatgtg gttgaagttt
     8701 ctttgacgta tgaaccaggt taccggtggt tcgaccgcag ttcaaaaaca tcccaaaatt
     8761 tcaccgttcc tcagaatacc tacgaagggc gtgtccatgc ccggctccgg gtagataaat
     8821 ttgaacgaaa tctcatggag ttgcttcatc acggtgtggc tttcggaggt gatgccattt
     8881 atggacatcg ctcccaatgg cgtcagtggg gagacccgga atttgaaacc cccgataacc
     8941 atgatgaccc cacatttatg ctggccagct tctatgccgc cttggcgggc aaggtcccgt
     9001 ttgtcccgac agaacggcac aggttggttg tcacgatgta tggtggaatc gggaaaaacc
     9061 ttgaccgatt ttccaccttc cggttgcccg gccgtcctac gggctacgag tgggaggcac
     9121 tgtcacgtcc tctcataccc ggagtggcct tcaacgaact gttccctcgc aagtacgcga
     9181 ttgcgaacct cacctaccgg tatgaggcgt tgtttttctt gtatccgtat atacgaggta
     9241 cctggtctgc gattgaacga gcccgtttta acggggatgg ctctattcgc aaccaggtag
     9301 atatgctgcc ggccgttgga gggggaattg tcagtggggc tccatggggt tcacaaatag
     9361 agatcaatta tagttataac tttgggatat accgtgaccg gcacggcccc aaactgggag
     9421 ggaatagtgt ggtggtgttc tggtcaaagc tactttaaca ccaagcgaaa aaaattcccg
     9481 catccgattt tctgggaatc agaatgagga tcttcgcctt ctttttttct cttccgatac
     9541 ggtcagttcc gtacttgagt cgggacctgt ggatcgacat gcacttctgt ttgaaccact
     9601 ccatagaaaa tcgtcgccag ggacagaatc aacaaaaaat aaaaggccac gcgcgccggt
     9661 cctaaaatag gggtcgtaaa gactccaaat ccgattaagg cacaaagtcc tgcgatgatg
     9721 agtaaaatga agccgatggt gagcatgggt acctcctttt ctggcaggca agggaccgac
     9781 tttaaaaaaa gatttccgcc atgggttccc ttcccggcca tcattccatc aaacgtcgta
     9841 tccgcaatca accaggttct gtcttttcct ttcaacgtat ttgtgcgcaa gagaaatggc
     9901 cgccgccatc gcccgcctgg agagaatggt gttggtgtct acaatatttt tatccatcca
     9961 gttcatctct gcattttccg aaatagaaaa cacccaactc ctacatagaa atttgaattc
    10021 ttgaatatgc aatcttcaaa agcttatcac catagtgaca actcttttag cccatccccc
    10081 taaacactta tcacttgtag gaattcatgg cgcagagaag aaaggagaga gaaacaagat
    10141 aatggacctc catatttcgg gagtgccgct agtattccct tcgccaggcc tacgaattac
    10201 tccatcaatc tgttacacaa gttcccgtac tcgatctgat aaggccgtca tggcaatttt
    10261 caaacgattt ggttcgcctc ttgccaacga ttcggcaaat ttcagggcct gactcgtggt
    10321 aatgttggcg ggcattggag gctcgaatgg gtccacaatg gcttgcacta ataccggacc
    10381 aggagtttgc aaggcctgtt cgatgatgga gccgcaatcc gccggatcat ctatagtcaa
    10441 ccctgtgcct ccgcacgcct gagcgatgag ggcgaaatcg atcggctgca gctcgcaacc
    10501 atattcagga tttcccagga acaccatctg ttcccattta atctggccga gggtattatt
    10561 cttgatgaca accactttca cgggtaatcg gtatttgaca caggtggcga actcagccat
    10621 gagcatggag aaacctccat cacccacaaa cgcaatgcat tgccgatccg gaaaggcgat
    10681 ttgagcggcg atggcatagg gcagcccgca gcccattgaa gcgagtgttc ccgagaccgt
    10741 atgtttctgt ccgcgtttgg cgggtatttg tctggcccac caggtcgtaa tggttcctga
    10801 atcacaggaa acaatcgcgt ggtccgacag ccgtttgccc agttcccagg cgaccacttg
    10861 tggcttcatg ggtttgtcct tcctggttcc tcgggcttcc attaattccc gccatttttt
    10921 catattggtc tgtgcgcgtt ccaaaaattt ccggtgttta ttgtggtgta acatcggaag
    10981 taattgttgg agtgtctttc gacagtcgcc gaccaggccg acttccacag ggtatcgcaa
    11041 cccgattctt gccgggtcca ggtcgatctg gaccgcacgc gcctgtcctg gttttggata
    11101 aaattccaaa taggggaagg acgtgccgat gaggaagagc gtatcacaat cctccagggc
    11161 ctcctgggag ggtgcggtgc ccagcaagcc aatcccgcct gtcgtataag gactgtcatc
    11221 aggcaccgaa gctttgccta gcaacggttt aataatcggg gctccaagaa tttccgctaa
    11281 ttgttctaat tcatctatcg cttcaagtgc tcccgctccg gccataatgg caatccgttt
    11341 tccgacattc agcacttctg cggcttcata caggtctgat tcattgggga ggcgggcgct
    11401 acgggaaaat acttccatcg aatgatgggg aatgttccgt tgggaataac ctttgtcggc
    11461 ttttgattcc tgaagatcca ccgggaaatt aatatgagcc acgccacgat agcccaaggc
    11521 ggtccgacat gcgagatcca cgacattttc aagatgtgtc gcgctcatca cccgggtgtt
    11581 atagactgtg acatcctcaa aggctctggt gagattgaca tcctgctggg catgggtatc
    11641 aatcaggtcg tgaaagtgat ggccggtgat ggcaagaacc ggttgttttt ccattttcgc
    11701 atcatacaat ccgttcaaca gatgaagccc ccctggaccg gacgtggcca agcacacacc
    11761 tagctttccc gtgtatttgg catatccaca tgccatcaag gctgcggctt cctcgtgacg
    11821 cacctggaaa aaccggatat ggtcttgtcg ttggcgaaga gcttccataa ttccattaat
    11881 accatctccg ggcatgccga aaattatttc gaccccccaa ttgtgaagct tttcgaccaa
    11941 cacatctgcg gcggttttcg tcatattctg agtttcctct cttgtttgtt caaggttgaa
    12001 ccagacggga ttgggatggg ggtttgcagg tcttaggaat tgtccgtata ggcgatggta
    12061 gaaaggaggg cattttcggc ctggcgggag tccaacctgg tatgttgcac cacaattggc
    12121 acatcggact caatcaggct tgcgaaatcc gtatccctgg gaatgggttc cgggtcttct
    12181 aaatcgttaa accggacatg tctcgttcgt tgagcgggga cagtaatgtt ataaggccct
    12241 atcggttccc gatctgaata aaaaatggtg atttttacat gcgcgggctt gttggatatg
    12301 ttcaggagac aggctgtttc atgactcgtc atttgaggtt ctggaccggt actgttcgcc
    12361 ggaatatacc cttccgggat tgcccatcgc gtggcgccca tcgcttgagt catgacccta
    12421 tctctttttc tgctggcgtt cgcggggctt ttccgacttg tccttggcga tggcgacgtt
    12481 cccattccct atccaatgca gcctcaagaa gataggaagg caaggatggg ttaatatttc
    12541 cggatagatg tgacccctgg gaatcactga tgttcatcgg ttcattcatg agaagattcc
    12601 tttccttgat taccttttaa aggtgaatga aaagattgca tgcccatcag ccgctcatta
    12661 tgatcccgac ggactctggg cgtgaacgag ttggggtttt ttaaccggcg gacggcggag
    12721 attttgccgc attgttggac ggcgtttccg gtttaattct ttaagagaga ccggctggcc
    12781 tttttgggct gaacgataaa gagcatcgat aattcgaaca tcatgaaggc cttcatcgcc
    12841 tgccggttcg ggaatcgtcc tgttcaaaat gcagtccgaa aatttgagca gttggggagc
    12901 aaattgatcg cgtttggaaa aggtttgctt tttctccttc ccgttaatgg tcaggtaatg
    12961 cgtaatgggc tcaacatact cgtaggctga tgtcacccgt agttgtccct ttgtacccac
    13021 gatccgaaac tcggaaacgt cggcggctcc gaaactacag atgaaggaag ccaaccgatc
    13081 ttcgggaaac cggagtgtca ccgcgcacat ttcttcaact tcctcgaatt taccgtttcc
    13141 ctgtccggcg ttggtggcat acacgctaag aggttccgct tgaaaaagat accgggcggc
    13201 atttaaacaa taaatgccga tatcccaaag cgttcctcca ccggtctctt cccgtaggcg
    13261 gatattattt ttgtctttga ccgggagact aaatgccgat tgaaagtacc ggggattgcc
    13321 gattttcccg gattgaacga tttggattgc gcgtagattg gcctcctcaa aatgaagccg
    13381 ataggccacc atcaatttga taccatgatg gtctgccgtg cgaatcatgg tttcacattc
    13441 cttgaccgag gtggccatgg gtttttcaca caagacatga accccagctt tcgcagctcg
    13501 ggtggtatat tcgcaatgca aatgattggg aagagcgata tacacggcat caattttttc
    13561 ccgttccagg cattcttcat attggtcata ggaatagagc ccgcgaatgc catattgttt
    13621 ccccaattgc ttcaattttt tttggtcatc ggagatgaat gccgctaatt ccgagttctt
    13681 tttcgcatgc gcgaaggccg ggagcacggc cacctgagcg atatatccta atcctacaac
    13741 cgcgtaccgg atagggtttg agggctttgt ttgtgtgcgt gatctctttt ttgtcgatgt
    13801 tttttttggt gccatgatgt gattccattc tcttaagatc ttaagaaaaa aattattggt
    13861 ttttatatca gggtctgctt tttagcaaaa ctgtggaaaa cccttgtcgg gaattttccg
    13921 ggtcgatatg accgggatcc gataccctca aataaaaaaa gttggagtag ggaaggttct
    13981 gaaaaaggaa tgaggcggga ggaactcgaa gcgggaaaaa gcacggaggc tttttcccgc
    14041 ttacagacaa tacggagata attagtcgtg tccagtttta ctcttcgtat ggacactccc
    14101 atccatattc catgcgggag agccgtcaat cttccgcacg gtcaggactt ttccgtttct
    14161 tttcagcaca atttttgatg cgacaatcac actttcccct tctttcatga tgcgtgaacc
    14221 ggtcacttcg actttatcgc cttccgtaac ctctaccccc tgagctttga ggtaattatc
    14281 tggtgcaacc cgcacacgct cggttccgga ggaggttttc acatccagca cgataagact
    14341 atcggttgtg gcgactcctg attcaaacag gtcattattc acgttttcca ccgttccatc
    14401 gactgtttcg acagtggccg ggttgaagtc cctggtatga atgccggctg cttgttgatt
    14461 gtggactgat gggtggtttt tagccggcgc cttttcgcca atcggcgctt cggcgacctg
    14521 aaccatcatt ttatcatctt gtttggtgag cttgacctcc gcccagggga tggaataatt
    14581 ttctgacccg atgcccaacg ttcccccgaa tgtgcctcca taggagagca caatataaga
    14641 tatctggccg gtcttttggt caatcagtaa atctttgacc gtgccaagtt ccttgcccgc
    14701 cttgtcaatg acggtgttgc caataagagt attggcgttg agaagaagtt cattagatgg
    14761 atgagtcgat cctcctgatt cactgagggc taaggacatg cctcctccga tgagagcaaa
    14821 ggctaatgcc attccgagcg tgccactggt tctgcgtctt gtgttcataa tgattaattt
    14881 cctttttgta agagtttcta aaaactgatg gtgatttggt gatttcatct tcttgattta
    14941 taaggtaatg gaatggtcac gagccaaaaa ccgtgcaatc ttctttcctc ctcttattaa
    15001 aagggctaac cattacgata aaggaaggta aatcgcccct gcggctaggg gtggctgtac
    15061 ctctcacctg gaaaaggaat agatttccga tgcgatgcta gttgttttgg aaaggtttcc
    15121 gtgatcggaa aggatatata ggaaatttgg ccgggattct gatcctttaa caattcggta
    15181 accgttgccg acttcccccc cggacctgtc gggactgatt cattaagtac gaacatcatc
    15241 cctccccctg ccaggccaaa cgctatgatc agtccgagaa ttcctttaaa ggtcatgagt
    15301 gggggcatgg tatgttttcc ttgtaaaaaa ctttcgtgag tatcatctgt gttttctgac
    15361 ttcctgattg taaagaaaat tggagaaaat gcgcctggag aaaacccttg cttccttgtt
    15421 ttttctccct gacgcatatg gccgaacaat atttgggaaa tcgcgattca agggttcttg
    15481 ccagatgttt ttgcttctcg tttcagggga taattccaag agaaatccaa gaataaaatc
    15541 ccaggagaaa gaggtgagaa gatgagtcga acaaggattg agatgggagt cgtgatgctg
    15601 ggtttcagca taaccctctt attgaatgca caaggattcc cccagggtct accagaatca
    15661 agtgcccagc aacttcagat ttcggcggat gaagctcaat cacatcaacc gaaccaattt
    15721 ggagagaaga aggaccttca gaagccccaa cctgtcatcc caccaaacaa tgatgctgag
    15781 atggtcataa agccggatgt tcctaccaac ccggaagcag tgatcactcc gccggtggtg
    15841 gatccggaga tggctgtgga tcctaccact cgtcagccca tgaccgacga aaaactggaa
    15901 gaactgacgc cagaggaatt agaggagaac gcccgagggg agaaataaac cttttacaaa
    15961 atacatgagg ttgctaaggg tttgttatca aaacggcggc ggcctgatgc ggtaacgtca
    16021 attttgtgag gcctacgcga gagtggtgct acggaggggc actacgactg ccattgccct
    16081 taacggatca ggctttattt gtaaatcgtt ttcttaatct ggagacccct tgggcgctgt
    16141 atatctgggt taggttgcct acaccctcgt atctttgaac gacatttcat ccgttcgctc
    16201 gaaaaattcc aacagatgtc ttgtcacgat ctccggttgg tccaacataa caaagtgcct
    16261 tgcgtccttc acacgaatca atctcgaacc ctgaatatcc ttcacgagcc tttctccata
    16321 atgaataggt tgaaaacgat cgttctgacc ccatacgacc agtgttggaa tcctgattct
    16381 cgagaggaga tgggtgattt ccgtcgtgag attggtattc aatgcggctg cattccgtat
    16441 gagtgacagt ttccccgtct cggtggtgta gggtgccaat aatccatcca atacttcccg
    16501 gttaacggag gaggcaaatc cgccctttaa ggcctgtcga agcaatcgaa ccgcaagcga
    16561 ggctgataat ttccggtgag cttcaggatg tccaaactgg agcatcaatt caattggcca
    16621 ggagtcataa cagaccgtgt tcatgacgat aagcgtttga acccggtcgg ggaacagggt
    16681 cgccaacctc aacgcgacac cgcccccgat atcatgcccg atgatcgtgg cacgagggat
    16741 tcctaattgt gtcatccagg aggcaatcat atgggcctgg caggcgatgg agcgatcaaa
    16801 gcaatcggct ttatcactaa aaccgaatcc gagcaaatcc gggaccagga cgcgacgggt
    16861 tcgcgccaac tcggaaaaga tcccgtgcca caaataaccc cagacaggga tcccatggat
    16921 gaggataaca gggggaccag ttcccgcctc cacataactg agaaaccggt cgttaagccg
    16981 gctaacgagt tgaagccgct gaaattcccg ccaggtcatg ttggtgactt cattatgggt
    17041 gagggtacgc caaccggaaa gggcgacatt tccaaatcct cacgagatcg ccaatccaga
    17101 gacatcagca cgatatttaa aatcaaaggt ggcaaaaggt gggtaaaatc ggaggttgtc
    17161 tctaggaagc tctgtcgttt ccttcccaga atcctcaact gatcctcaga caaggttagt
    17221 cgaggaatga cctcgcccgc catctgttga gccgcttcct gtaaggccaa cgcggcttcc
    17281 atgtatcggg gttgatgtat cacagatttt aggcgatgcc agactgaaag aaaatattcc
    17341 ggccatagtg ccaacgtccg gtaatcgctg ttaatcctgg ttaaacccaa tgttcgttgt
    17401 aaatcccgaa atgttttccg gagagtggca ttgtccggag tttcctcgat catttccatg
    17461 gggtacatgt gacggggaat tcctcgcgta atcagctcat gatcctgttg gcgggattgg
    17521 aaagcggtca tagtgggatg atggcaagcg tattccaccg cggacgtgat gagcaggagc
    17581 ttgggattga tgtaatggta aagttcgaga gcggcctgta tgtgaaaaac ctggctttcc
    17641 cccaacctga cggacgaggc cacctccaac ctgggaagtt tctcagcaag gcgggcagct
    17701 aacgcccgga gttgatcgga tgaatcttcg aatacacggg tttcagcgac cggtcgaata
    17761 gactcccata agacagggaa aaacgttgga taggcggccc atgtgcgaaa gttgagattg
    17821 actccggaaa cacgaaagac ctgtttaatc tcatgaaaaa tgctgtccag ctctttctca
    17881 gcctcttctt ctggtatggg atggtttttc ataacacaca aaaatgagtg gggtgttaat
    17941 ccaagtgtgg gccacgcggc ctgaattgac tactagcaga aaccctgtag tttcaacgga
    18001 tgccagggtt tttgttagtt gttgatcctt ttgtccttgc ttatcttctg aattacacag
    18061 aacggcgccc cattgcattg gagcgcgtcg tacctcagaa cagaatggag gccatggatg
    18121 atgacttcac caacgaccac acgagttgag gaaaataccg acgctgacat caatgaggaa
    18181 atctataacc gcacaaaaaa taatgttctg ttgtatgcct cagccgggcc ttcggccatc
    18241 gatcgacggt taagggagct agaggtggaa tgggatatcg aaagaatact ggaagccaat
    18301 gctgcctcat tttcccttat gggcctggca ttgggagctt ttattgatcg ccgatggttt
    18361 atatttcctg ccgccgtagc cggttttctt ctgcaacatg ccatacaagg ctggtgtccg
    18421 ccggttcccc tgttccggcg aatgggaata cggaccgcac atgagattca acaggagcgt
    18481 catgcgttaa aggcattgcg gggagattat cgggacgtgg ccgaattaaa ggccgaagtc
    18541 aatggggcgg acgctctgca ggtgactcga gcatttgagg cggccggaaa taactcctcc
    18601 acctagatgt ttaggagcat ttttggcaca atcatcatcc acaactcatg atcattaaac
    18661 aatttcatgt ggaaagtctg gggcacgcct cctatcttct cggttcggaa gaaaccggag
    18721 aagccttcgt ggtagatata cagcgggacg tggaacccta tctcaaagaa tcccgcacac
    18781 agggaatgac gattgcgtat gccctggaga cccatcagca caacgactat gtgacgggaa
    18841 ttcgtgaatt agccgaacgg attcctctcc aatgcttagc gggtcgacat gccaagctcg
    18901 gatatgacgc gcgccccatg gccgatggag agcggttaac aatgggagaa atagattttc
    18961 aaattctgca taccccggga catacacccg aacacatcag cgttctggtg acagaccgct
    19021 cgcggggtaa ggaacccgtt ctgctcctct ccgggggagc cttgttggtt ggggatgtgg
    19081 ccagacccga tcttttgggt ggaacggaag aagcggctca tcacgcggag gtcatgtgca
    19141 aaatgctcca ggaaaaaatt ctgccgcttc cggactatgt ggaagtctac cccacgcatg
    19201 tgagtggctc tctgtgtggg gggaatattg gaagcaggtt gtccacgacg atcgggtacg
    19261 aacgccggat gaatcctctt ctggccgatc tgtccaacaa agatcatttc gtcaaacact
    19321 gcctggattt aaagcatctt cccggtgtgc cgccctattg gcccaggatg cggaaaatga
    19381 ataccgaagg cccccctctg cttggaacca tgcccgaacc gcctgcactc acaccggacc
    19441 agttttgccg acaacaggaa gaagggtgcc ttgtcgtcga ttgccgccaa gcggaagggt
    19501 ttaccactca cattcccggt gcgctgaacg ttggattggg atcatcgttt gcgacatggg
    19561 cgggtacggt catcccggcg gatacccggg tcttacttgt tttggatcat ccggcagacc
    19621 tttgggaagt gtcctggcag cttcttcgaa tcggatatcc tacaccggca ggttggctgg
    19681 cgggcgggat gttcgcatgg cgaacggcag cgaaacccct ccatcagtta acacagtggt
    19741 ccgtgtggga tcttcaggct cacctagaga aagattccga tctggaggta ttggacgttc
    19801 gccagccggg ggagtgggct gccggtcaca tcaagaatgc ccgatttgtg acgggcagcg
    19861 aactcccgca acgtgttcag gaaatcagaa aaaatctaca ggttgcggtc atctgtggaa
    19921 gcggataccg ctcgtcggtt gcggcaagtt accttcaaca tcatggctat ccaaacatcg
    19981 tcaatgtgat tggaggaatg agcgcttgga aaaaggctaa ccttccgatt atcaaggatt
    20041 aatgccccag tccggcccgc ttgagggcgc cacaggattc cgcaagaatt gttctattgc
    20101 aaaaggcttg agcccccgga tggttggtgg tgaaattgac gtgttaagtt ggtttgcttt
    20161 cgccaccgcg gatcctcttc ggtaatacga ctccttttga gcatttggcc gctcttgttg
    20221 ttttcgcctc ggaggattct tctgccatcg taatttcaga catttatctg gaggatatgt
    20281 cctggttgca ctctatgaat gaatgcccgg tgatatcgtg gaaagaagat gacgacatac
    20341 acatcgaatg gggtcaggcc cataaagggc ttgatgtgta ggttcccaga ggataataga
    20401 gatgttcaca agaaatgatt aaatgtcgat gtgattcaaa cggagataaa aaactctgtc
    20461 aacaatgggt gtcggccgat cggacgatct ccccttcgtc cgatcggctc ctacccctca
    20521 ctagatggca ttccctggcc ctagtgatgc ttgtatgtca tgaagctcct catatcaatc
    20581 tcatcacata ccttgttgac gtttttccct ttcttctgcc tttttatctt cacccgtata
    20641 tccttgtgag gtgtcgcctg ggggaaaggt gctagggctg ccaaaggttg aaccttcttc
    20701 acctacacca cccgggcggg cttctctttg ctgatcctcc gtaggttcag tattccggtt
    20761 cgactgactt tttgactcaa tcgtcccctg attttgctcg ccctgcatgc ttggggaatc
    20821 tccagcttga cgctgttggt cgttcatccc ggcctgacct gcaaacacgg aaacaccgga
    20881 aaacacgagg aacgcggaca tacccatgac ggttaaaagt tttgtcatgt cacttctcct
    20941 ttgaatagtg gttattatta attaaacggc tcagactgta agggttaaat gttgaaggtt
    21001 ttggcctctg gatcctagtg tgaagctcag agggctcttt ccaatttatg gacggtatcc
    21061 ttccgggcga tcatcatatt ccttcatatt ggatctcctg gattgatact gatcgtcgtt
    21121 ctgccgttgg gaatcataat ccctccgcgg ggctgtcgtg ttgtaccggt cagacaactg
    21181 ctccatatct ctgtaatttc cttcgcgatt ctgggaatac atggaccttt gcctatcacc
    21241 ttggagatca tcatagttcg gcctgtttga acgggattgg ttcctgtatt cccgatcgcc
    21301 cgaattattg taatttcttc cctcgctgcg agaatactgt tcatttccgc gatcccgata
    21361 atcatccgaa tatgatcctt gtcgatttcg ggtagttgaa ttctgctgat cagccccttc
    21421 atattgaaag gctcgatcat gtggcggaaa ttcggttggg ctgaaattgt tccgtttggt
    21481 ctgatagtct tcagcattct gatcttgata gtgcctttgc cgatcatctc ccgaacggct
    21541 catgcgtgac cgatcctgat cgtaccggtc atagccttcc cgattatttc ggaaagttgg
    21601 cgtgtcttcg tagtagcggt ctcggacttg gtctctctca tctagtcgct cgttccactt
    21661 ggccaaattc tgccgttcct gttccgaaag atcccaattc ttttgcacat gggcgccgac
    21721 attttcacca tcattcagaa tgggaagatt ccccaattga ttctgggtga ctttcatttt
    21781 gaaggattta ttccctgatc cctccaatgc ctgccagggg accgaaatta actgtccgtc
    21841 cggtttcctg ataatggcat aggcaatgtc tccgttgtta ttcgtcatca ccctgtagat
    21901 actcccgatt tccttgccat cattagaatt gaccgagtaa tgagcatcct tatccgagat
    21961 atccaacccg cctaattgcc cggctggaag aacaaagtac tccccttcca ctccggctgt
    22021 tccatagtag tcctcactat tccccatgga ccgcacaaat gtggctgtat tgttttcacc
    22081 taatttggct ttgacaaact cccctttttg gaaattacat tcgccaaacc taaagccctg
    22141 ttgttgggat ggctgtgaat cactggacat ccctggagag ctctgaccgg aagcccgtcc
    22201 actcggattt tccaaaagat tgctactcga ggtttgagcg gaggtagagg agtctcctcc
    22261 gggagaattt tgggccattt gatttttccc ggaagtgctt tgcgcagaag atttcccggt
    22321 ggaattccct tggtccattg cggaatgttg atttttttgg gactcaccac tcatggaaga
    22381 cgattgtgat gtttgagttc cttgaggaca aagctgattc gtttgatcag tgatccggaa
    22441 ggtaattcgt tctccatcgc tagtacgcaa ggtgattttc tcaccattct ttttggaaat
    22501 ttcgccctca atgatttgtt cgatggcgtt cgaatgcccc cggtccccgc gttgtgaacg
    22561 gtcctcccga gacatgccgg attctgccaa caacgggaat gtcagcagtg gaatgccggc
    22621 gagaaaaatt gttttgacca tgtgacttcg caacttcatg gtttgtacct ccctaggggt
    22681 tggggttgaa aaatatgatg atggtcatgt tcgtcacaat aaatgttttt gtgcccggag
    22741 caactgttga aaaccctagc ggtttcccaa gggtttatca cagttcgacc cgatgggacg
    22801 ccatgctata ccgaatgaaa cggttccata ttattcattt aacgatttgc aaggaggtcc
    22861 gtatgactac caaaattcga caggtgcttt catccagttc catcaccggt gacacagtgc
    22921 agaatatgga tagagaagac ctcggagaaa ttaaagattt aatgattgat ctgtcggaag
    22981 gccggattgc gtatgccgtc ctttcattcg gaggattttt aggaatgggt gataagctct
    23041 ttgcgattcc ctgggaagcc ttgagtgtgg tgcaggaaga aaaagtgttt ctcttaaacg
    23101 taccaaaaga aaagttggaa caagcccctg gatttgacaa ggacaattgg cctgatatgg
    23161 ctgatgtgac ctggggccag agtatccatg aatattatgg gtacaatcct tactggggtt
    23221 aaataaaagg aatgaacatt ctgaagacaa atttgacatt ggatgaatgc tgctcagaaa
    23281 gaacccgccg tccggcgggt tctttttttt cgaatcacac ctcctcttca atagttgggg
    23341 tagcgtcaat tttcttctga attatcacgg attgacccat tccttcccga atccacaatc
    23401 ttcttccgtg gttactctaa agccattcct gcaggataat aattaaccaa gatttcgatg
    23461 ggttccggca tggattacca gagatcagcc agggttttat agagttggtg ggatgaattc
    23521 aagcatgtaa tcttttaatg aggaaacaca tcaacagagg aggcatggac atctatggca
    23581 gataccagtg ttaaaaaagt acaggcggat cattctccca agggttccat gggacagaaa
    23641 tatttagcct caggtgttgg attggcaatg cgattatggg aggaattacc agtcgggtac
    23701 acacacgaat catctaccag ggattatgaa acaatcggat atgtgatcca gggacgtgct
    23761 gagcttcagc ttgaagggca aacgcttttg ctgaatgcgg gagaatgttg ggttgtcccc
    23821 aagggcgcga gccacagtta tcatattctg gaaaccttta ccgccgttga ggcgactcac
    23881 cctcctgcac acattcatgg gagggatgaa gtctagctcc agtttgtctt ggttccccgt
    23941 ggcgttttgg aaagcaaagg aattcagcct ccgactgggg gaaggggagt gtctggagca
    24001 tgctgaacct tttgataaat ttccaacgcc tcgggcatcg cctctttaag acgtgtaatc
    24061 cttgtctcat ggctgggatg ggtggataaa aattcggagg gtgtcccttg gctaccttcc
    24121 tgcattcgtt gccagacccg aacggcttct tgagggtcat atcccgcctt ggcggcaagg
    24181 agaatgccaa tggtatcggc ttcggattca tgggtccgac tgtagggtag gagaacgccg
    24241 acttttgctc ccaagcctaa ggcggccatg gtggcttgtc ctactaccgg tcccgcacca
    24301 cttgcgccca gccctaccgc cgcagcggag agccccgctt gagaaagtac ttcctggctc
    24361 atacgctccg aaccatggcg tgcgagggca tggaccacct cgtgtccgag aatggccgcc
    24421 agtcctgcat cgtttttcgc aacggggaag attcccgtat acacagcgat cttcccgccc
    24481 gggagggcga aagcattttt ggtctggtca tctttgatca ccgtcacttc ccactggaat
    24541 gccttggcgt cttccgcaaa cttggattct ttggcggcct gaataatcct gtccgccaca
    24601 cgatttacgg cttcttgttc ctcagcagat tgagattgct ggaccttagg atcggaaaga
    24661 atttgttgaa aggcctgctc tcccatttct gcggcctgtg attgagggac caccacaagt
    24721 tgcgatcgat cggtatacgg aacggtttcg caacccatgg ccataccggt caacatacat
    24781 aaaacacaga aaagtttaac ctgctggaaa cgaattacgc tgaatgggtt gggcattggt
    24841 tttctcactt taccggaaga aattatattt ttcgttcaca cggttttgta cgaaggtgca
    24901 atagaaaatg atgaccgtac cgtataggga aatgcaaaag acaagtgacc tgtgattggt
    24961 ggttaaaatg tccaggttgc gaacaagaag atttgttttc gcaagagaat gctttgaatg
    25021 caggttggga aatattagac gactttaaaa gaatcgagga cggcaggaat gttggctcat
    25081 tgagatcatg atgtggcctg atggggagaa tatcaggatt gaaattgccg aatggcaaac
    25141 tcaatacggt cctggacgct ggtaagatcc cgggcggatt gttctaccag gtgtagacgg
    25201 gggagaagac cggaacaatt ggcaatttga atatttaacc cagggcgggc gttcacctct
    25261 aacaacaaag gaccctgatc tctatcgaga acaatatcga ctcccaggta ccctaactta
    25321 aaaatatcgt aacattggga tgccaatgtg aggagatcct tccaattagg aatgacaaga
    25381 cccgtgacgg aattattcgt atcaggatgt tcactgacaa tttcatttaa tagcacggct
    25441 gtcttggtag taccggtcgc catattgaca cctaccccca aggctccctg atgcaaattg
    25501 gctttgccat ctgacatccg ggtaggcagg cgaaccattg acataacagg cacgcccaga
    25561 aaaacaataa tccggatatc cggaacaccc tggtaactaa tagattcgaa gatcgggtca
    25621 aaattaacgc aatattcaat cagtgcggta tcagccgccc cacccagact atacatgcca
    25681 cttaatacgt tggaaatatg atgctgcagt gtatccaatg agaggatgat cccgttggct
    25741 tttcgatatt tatccttaac ccgccctctg atgaccacga tgccatcgcc tccgcttcca
    25801 tgagcgggtt tgatcacaaa ttctgtatgg gtatttaata atttgggaag atcgcggatt
    25861 tgtctttcaa tttcaatttt tccgtacagt tcaggaacag ccatgccggc ttgggagcag
    25921 agtgttttag tctgggcttt atcgtcgacg aagggataaa accgacgttg attgaaaccc
    25981 agagtataca cggcatttcg tttgttcagc cccagaatgc cctgtttgga taagttgcgg
    26041 aatgccttga acatgttatg aggatttcaa cagctcttga aaccggcgga cttcaagtag
    26101 gcggtaaccc gtgtaccgtc ccaaggcaat tgtacaggct aagaggatga ggagtaattc
    26161 aggaaaagta aaaaacacat aatccattgt ttcaagactc atgagtaaat aggtgaggga
    26221 ggccaccgcc aaacttcctg tggcctgctg aagagcttca ccagggcccc gttcatccca
    26281 cacaatcgac atgcgttcaa tcgtcatggt gacaataacc ataggaaata aggccacgga
    26341 aagtcctctt tcaatcccca ggtgatgaga gaggacactg accaccccca tcattaagac
    26401 cacgacaatg aggacggcag cgaggcgggg aaccagcagc aattttaaat gttccaggta
    26461 aaatcgaacc gctaagccta acgcgattaa cagagaaaaa agggtgattc cccaaagcaa
    26521 ttgagtttct ctaaatgcca acgcaatgag aatgggcatg aacgttccaa atgtttttaa
    26581 tccgacaaca tttctcagca ggacgaggag gaaggcgcct acaggaatga gaataaggat
    26641 gtgataaacg atctgcttct ccacgggcag gctgaacagg gaaaatttca accaggccgg
    26701 attcatgacc atgccacggg caaccgcagc ttgtatggct ttttcctggt tagggctggc
    26761 ggaaagacga aattcaagtt ccttccctcc atcaatgtgg aacaagggtg catcgccccg
    26821 ccaccaggaa agatagttgt ccggaattcc ggttttgacc tcctcaaggt cgaatggtac
    26881 ccacgtatct tcgtcaaaga cttcaatcca ctctttcaga ttaacggttc gagcctccct
    26941 gcttaattcg attccgtgaa ccacacgggc gggggttcca tttaaagtga taatttgagc
    27001 gatggcttgt tcagcaggtt gttgagagag taaaagggct tcataggaat ccggattgat
    27061 tttggtaaat cgtcgaatca ggctcaatgt taacgaggtg gtgtctgccg tttgtttctt
    27121 gacctcagcg agaagggcta tggccgcttg aagaaaggcg ccttcaagag tggggaggtt
    27181 cccctgcgaa tcctggggct gctgaagagc atcggtaaac tgttgaaatt ccgtttccca
    27241 attcttcatt tggcgaatga tgccgcggta gtaaagaatc tggacccctt ctgcctgacg
    27301 tttcgaccag gtgaccagcc ggtttgggcc ctctactttt tccgtgacac catattcttg
    27361 cccgatgaag gattctgtgg tgatgatata cgggaggatg gatttcgggg taaacatgga
    27421 aacctgcaca ggcccatctg ttgcttgaaa gctgatgcgg ctctccacat tccaaacctc
    27481 agatgtactt tcaggattga gtgggaatcc taacacgacg actttgtaca gaaagataga
    27541 aaggcccaaa agagtaagga ggaaaatcag agaaatgaaa tgaaggcggt tcactttttg
    27601 gattgagagg aacagtcagg ttttaaaaga tgggtggtgg aggaatcgac aataaaatgc
    27661 cctgacaaaa acattcgccc gattaataag ggatatttgt agttactacg atcggtcaga
    27721 ttcacttccg tttgttcaat atgtgtaccc aggcagaggt ctaaaagaat gacggggcgt
    27781 tcggaggatt cccgttcatg ctgtttgatt ttgacggtcc gatgaacctt acgttcgagg
    27841 gttgtgtgtt ttccgtcctg ggcggtgatg tcaaatctga cccaacgttc attatccttg
    27901 gagaacgtgg ataaattttc ggcatgcaag gaggcgctct ttgctccggt atccaatttg
    27961 gcttcaagac gaattgaatc gggaatgagt atgacctctt caacccagcc ggcacgtatg
    28021 ggatggtcct tggccataag gggtgtagga ggttggaata aggctccccc gaaggtcaac
    28081 atgaatgcca aggtgagccg gccattcaat tccgtcatgg gcagaatcac gcgcctaaac
    28141 aaaaatgaaa gatgtggata aatccaaccc cggtcaaggt tttttgggaa tgcgtaaagg
    28201 cctgaattcc cgcataaatt atcataaccc cctatgaaaa tatagtttaa cggagagaaa
    28261 gaccacgggg ggaaggaaaa aaatgaaaag gataaatttt tcaagccaat cttgaagaag
    28321 ccgaagttcc ttcaggtggt gataacttgc ccctcgagag gcctgatccg atttatgagt
    28381 ttcgcttcat ggtccaaatc cgatcaaggg ctgttttcaa attttcaaga gaaaaaggtt
    28441 tgaccagagt ggcggttgct ccaaccgaga cagcatgatc gatgaagaaa ggatcggaat
    28501 gcgccgtcat gaaaatgatg ggaatggtgg aaatgccgga tgtcgttcgt acggtatgga
    28561 tgaattgaag gccattcatc tccggcatct gataatcaca catcacaaaa tcgaaccgtg
    28621 agtagctcaa tgcgcacaga ccttcatgcc catcatccgt ttcctgacac acatacccca
    28681 acgaggttaa tcgggttttt aaaatttccc gatgatccgg gttgtcctcc acgattaaaa
    28741 ttctgccttc aggtgaattt tcggagagtg gcggaaatgg actgatgggt tccgatgaat
    28801 agattgggaa tgacgatatt gtaaaattct ttattgtgct catttgcatt ttcctctccg
    28861 taagtgaagg gttgcttgga ttcagctatt tcttcctaat cgccacgtct ctgccgctta
    28921 cgagggggag tcctctcctc ttttacgatt atcactattt catcagaagt gccagggaca
    28981 aatacggcgg aaatatgggt tcttgtcttt ttcgcatggc aggtggacgg aaaggttctg
    29041 ccgagcatag gacggtcgtt gggcggtttc agcagtaacg ctatgaccga tgaaaaccgc
    29101 cgatgaatga aagctctcca tgtccccgtg tgcatggtga gggaactata gacatttggt
    29161 tttttgggat ggtcgatttg tccattggct gaatgtcggc cacgtgatat ttgagaaata
    29221 cattcagcga ttatgaacct catgctgttg ttcaatccta tttggaaaac ccaaaggttc
    29281 ttcaattaat cggcacattc acatttggag aagggggtct gaaaagtatt tgaacgtaaa
    29341 gacgctgctc tctcaagctg gttgagaaag ttgacagcac gaagatggat ggaaatggac
    29401 ctaaccaaga aaggggaatg gtatgttacg agatctcgat aaaatcaata attgcgtgtt
    29461 acaagggaca gacgggatta taggctccgt ggccgatgtg tatgtgaatg accagacgtg
    29521 gaaaattcat tatctggcgg ctgacaccgg gaactggcta cagggccgcc atgtgttgat
    29581 ttctacccag agttttgtgc atccccatga ggacattggg tcattccggg ccctagtcaa
    29641 taaggctcag gtcgaggagg ctcctaaatt tgaaatagaa aaacctattt cctgggaaaa
    29701 ggaacgtgaa atctgcaact attatcgatg gagtacctat taccctattg acgaaaggga
    29761 actttcggta tttcccggac ttttgaccag cgcgaagggt ctcaaagatt tttccctcca
    29821 ggcgacggat ggggaaatcg gaaaaattat taacttcctc attgatgatg ccaattggac
    29881 cgtacggtat ttggtcgtgg atacctcgaa atggttggcc ggcagaaagg tgttaattag
    29941 tccaatgtgg aataagtcca tagattggct ggagagaatg atggtcgtgg acctcaatca
    30001 gagccaaatt gaacagtctc cggagtatga tccagctgcc ccaatcgaac gagtctatga
    30061 aattaatctg tataaacatt acgggaaacc acattattgg tagaacaatt gagaagacat
    30121 tccaggccgg aatgaaggaa ggattcgtga gaggaacatc tgattcaaaa catttaatca
    30181 ggataatacc catgtggcga tccaggcagt ccctcacgct ccgattcttt tcctggtcac
    30241 cccacacatg ttcttttgca tattggtgga gagacctggt gctacccaag gcttacctat
    30301 tgcattgagg tacacaaaag tccttatgta tgaacatctg attatttttg gagccacagg
    30361 ggacgtcacg aagcggtttg tttttcctgc tttggctcac gtgttcgcca accggggtct
    30421 tcccagggag catagaatca cgggagtagg acgacgagat tggacgactc ctcattttca
    30481 ggagcatgtc tccgaattgc tcgataacca tgaatcttca ccacccccaa gatcccggga
    30541 aggattcttg gaatctttgg actatgcacg tgtacaggat atttccgacc ctcgacagat
    30601 tcaggccatc tttagccagc aggcagggtc aacgctcctc tatttagggt taccgccaca
    30661 gatgtttccg tcggtgttgg aaacatttcg acacgtgacg ttaccgccgg aatcccgtgt
    30721 catcatcgaa aaaccgttcg ggttaagcta tgcccaatca atggaactga acggacttct
    30781 gcatcagaaa ttccctgaag gcatggtatt tcgtattgac cattttttag gaaagccggt
    30841 ggtggacacc attctcggtc tccggtttgc caatcctgtg ttcacccctc tctggaaccg
    30901 tcatcacatc aaaaagatcg aggtgatctg ggatgagacg ttggcgctgg aagggcgtgc
    30961 agactattat gatcacgccg gagcgctgaa ggatatgatc caaaatcatc tccttcagtt
    31021 gatggctgtc ctggcgctgg gaccattgga gtccatgtgg tctcctgaat ttcgggataa
    31081 aagagtcgaa ttgttcaagg ctattagaaa accctcaaag cctgaaatcc gagcccagac
    31141 cgtacgggct cgttacgaat ccggggaaat aggtggtgtg cggattccgg gctatacaga
    31201 aaacagggga gtggtttcag agcgaaacac tgagaccttt gctcaagtga ccttgtgggt
    31261 cgataatgaa cgatggcagg gtgtgccttt tgtgttacgt tcgggaaaag ccctgggtcg
    31321 ggaccgacag gaaattctgg tgcattttaa gggtggacag agaagtaccg aatcccttct
    31381 ttctgaagaa ggcggcaata tcttgcgaat aaatctcggg agcgaaaaag tgaaccttgg
    31441 aatatttacc atccatgaac ccggaggaat tacacctgaa ccaatgaggg tggacgaccc
    31501 cgtgtctcct gaacatcttt ccccctatga acgcctgttt ctcgaggcca tgtccgacaa
    31561 attgcgactt tttgtgcgaa atgacgaggt agaagagatg tggagcatca tgcagcccat
    31621 tgccgatgcg tgggcggaaa acctcgtgcc cctgcaaatt taccgggctg gatcctatgg
    31681 tccatctatg ggagatcaag gcgacgttgg agagtccatg gagtccaaaa tgtctcgaac
    31741 gaaagaggaa tctcctgccc gcgacccatc ctcctcatat ctctaggatt ccatcagaag
    31801 tctggtcgtg atggtgcgac tgttacttgt ttttcaacaa cggcatgaac cggaatgtta
    31861 aaaaattcaa cacgcttagc accagggcaa tttcgtaacg gtcccaggcc acggccattc
    31921 cgatggctcc ggtattccaa atgctcgccg cagtggcagt cccggccaca tttcctctct
    31981 ctttgaggat ggcccctccg ccaataaatc cgattccggt taagatgccg tatatgacac
    32041 gagcttcggc atcggtggaa ttcagcacat tgatccctgt cagcatatac gcgcaagacc
    32101 cgactgcaac caaagggaag gtgcggagcc cggcaccgcg cgattcatgt tcccgatccc
    32161 aaccgagagg aacggccaaa agaaaggcaa tgagcagatg atacaaatgc gaccagacct
    32221 gatgccagtt cagatcgatt aatccatcca ttgggaatcc ctcgttttgg aggccggaca
    32281 agttggagac gctgggacct tcgggataaa aatgtcacgt atcatgcggc acgtcctgtt
    32341 ctttccggtg gcgtgtgatc aaggtctcca atggttcttt aaatgttaat gtgcgataag
    32401 ggaatgggat ctcaatcccg gcggaatcta aggactgttt caccgcagcc acaaccttgt
    32461 ccttggattt tctgatgtcc accgggcggg acccagtcca ccaggtcacc tcgaagtcaa
    32521 tgctactggc cccaaaaccc tgggcaaaga cttgtatgtc tttctcctga ttgaccgtgt
    32581 ccaatccttc taatgcgcgc cggattacat cgcgtccagc attgacatcc tctccatagg
    32641 cgatgccgca catgatggtg acccggcgca gatcccggtc tgtccgcacg atgaccggat
    32701 ttttaaacag cacgctgttg ggaataacta ctcgttggcc gtccgtttga cggatatggg
    32761 tatctcgaat ggtgatttct tctacaaacc cttccaggtc ctcgcattca atgacatccc
    32821 ccaattgaaa cggctcgcga aggaggatca agatgccggc caggaaattt tcaaaaatat
    32881 ccttgaaggc aaatccaatg gctatggatc ccaaccccaa tcctgtgaga atttttcccg
    32941 gcgttaagct gggaaagata ataatggccg caatcaatat tccgactatc caaatgccga
    33001 tgtacacgag tttctgaagt aattccaaaa gagattcccg tatgccaaac cgagaaagca
    33061 gtcgggtcaa tagggtttcc aaagccttgg tgcttaacca ggtcaacgca aggatcacga
    33121 tagctgaaat cacattcgga aggtgagcca atgcctggct tcccatttcc cgaagactgt
    33181 tcaatataat gttgaggggt tccatgtgtt atccggcaac aatcaggtca gacctgagga
    33241 ttgaggggta ataatttttc catgcacttc cgtaatatct cccgactgaa ggcggggaat
    33301 aattaactcc ttcgccagga ttttcaaaca ggccgcaaag gggatggcca gaatgaggcc
    33361 caacactccc cctagagctc cgccgattaa gacgacgagc agaatcgttg cggcactgag
    33421 gtcggtggac tgactttgaa tccagggcgt gagaacccaa ccctcaatga attgaacacc
    33481 cagataaact gcgctgggcc agacgaggac ttgcatccaa taatcgtcga tagaggaagg
    33541 ccccatggac atatctaaat atttcaacag gagagcaata ggccacatta aggtggcggc
    33601 atagggaatc aggctcaaca cgcctgcgca aaatcccagg agaacccaat agggcattcc
    33661 caccaggtac cagccacctc caaacatcac gctcataatc aacgcaatga gcaatcgtcc
    33721 tctgaaaaat tctcctatag ccacgtccat ttctgtcagc agcttttccc agcggggatc
    33781 gccctgagat tcgagcaaac gccaaaaagt tttctggatg gagggaaaac tccaggcaaa
    33841 aaagaaaaag taaatgacga ttaatgaaag ggaaaatagg aaagaaccca gtgtcccgat
    33901 tatgatattc gtaaaataaa acacctgccc ggttcctgag aaaaatgttt tcagcgtggc
    33961 gaagggatta ctcttaatat tggtggaaac ttcataaagc ctgcgggaaa tgtcctcgag
    34021 gtccacctca taccggttgg ccaaaatgga aagatatgtg gggacttttt caaagaaatg
    34081 ccgtagttga tcgacaagaa gtggtcctat ccacagaacg aggccactga acagcaggca
    34141 cataatcatg gccatgaagg ccacggcgac ccaccgcggc aacctccagg cctgttccac
    34201 gtaatccagt gttgggtcag ccaggtaggc caaacccaga ccaatcaaaa ccggaacaag
    34261 aatggcctgt aattgcaagc ataaccagag gaaccacaca accactccga gtaaacacaa
    34321 ttcacgaatg gccacgactt cccagagatg cagatttctg gaaagttttc ttctggtcgt
    34381 ggaaggagtt ggcattggca tacattatgt ccgcaatatt tttcacggca tcagagatcg
    34441 ttggaacacg aactctgaat aagctagagg tttccctgct gtactcaaaa atttctacta
    34501 aacgctgaga cagctttgcg agtggctgaa ccatgcacgc gcccccaaca tacgccgggc
    34561 gtttttggct ccaattaaaa gaggcgtaga aaggatgagg ctatgttccc tctggttcta
    34621 cagaccttgc ttttcctact ttccatctaa atctctaaca aagttcctgt tgatcctata
    34681 tagataaaac ccaaccgtct cactcacaga tgtctattta aaaaataatc gtcttgagac
    34741 atgataggaa aatcaacgat tcaattcctg gatacgattt cataacggga ggagaaagtc
    34801 gtgtaccgga ggggagagaa aatatgagag attatccaga cgcggggagc cctgatagag
    34861 ggaggccgag ggtagagtgg attcggagag agaccggggg gtatgggtta cacggctcca
    34921 aagaccatcc gcctgtcaca ccacctccgg aacctgaaaa aaaacgacca cccatgggtc
    34981 ctccaaaccg ggaccctaag ccggaaccga ttaacgatcc gcttgttccc cctccgccgg
    35041 gaaaggaccc tttagaggag cccaaaccca tcggtgatcc tcctgatgag acggatcctc
    35101 caatgcgtat gggtgtcatg acaagagttt gttctaatca agaggcagcg gatttttttc
    35161 cctatagaga cagcgtttca ttcagtgcct accgtcatcg caggatctct aataaccatg
    35221 actcactagg aaggagcctt ctatgacgat attaaaatgg gcggcaattt tctttgtcat
    35281 tgcgctcatc gcgggtggat ttgggttttc agggattgct gagggagctg cgggaatcgc
    35341 acaggtatta ttttacattt ttctcgcaat ctgcgttgtc atggtgctga tcggagtatt
    35401 tgtggccaaa aaaattacat gaacccgttt gatcaaccgt ggagacttca aggaataggg
    35461 ttcattggct ttattgacga ttctggcgca aatatggagg caaggccatg aaggaatccg
    35521 ggcctagaca agagggatat tatgaccaat cacgagaaag caaaattgat tgcgccctgg
    35581 attaatcctg cggaacgggt cactgtcgat tttaaagatg agataggttt gaatgctgag
    35641 gtcttcgggt gcaccgaaaa cgtcgtccat ttattatttc aagaagcttt cccacatata
    35701 aaagaacaaa tcaccattcc gttgagagag gtggaggttg gagaggatca tggacgctat
    35761 acacgggacc ccgatacacc tcttcaatgg cgattgcgtt tgcaggttaa tcgaaatcgt
    35821 cctgaaggca tgtgaagtct tgccaaattc accctttccg aactcaagca tgtcatcggg
    35881 acaaggaaaa ttggacgatg tggaatatcc tagcaacaac ttgtccatac aatatttcgc
    35941 gggtctccgg tacattaaaa aagaaggagt gaatgggacc gctggcagaa ttatctcgcg
    36001 atgacggata caagcgggat cctctgtcat aatttattaa ctcacttaga ataaaggagt
    36061 agaaatatgc caatattatt gtggttatta ggagttcctg tcagtctcat ccttgtacta
    36121 tggttgttcg gggtcctttc tttttaaatg cctgtttggt tgaacaggat ttatgaatca
    36181 gggattgtgc aaccttgtga atcccgtcgg gttggaaggg tacatccctc acttaaaccc
    36241 taacccggta agcttttctt gtacacggaa gcgggcctgg gacattccat ttgaggaaat
    36301 gtccgggccc gttttcatta aagacctcgt cgctgcctca gatgtagatt tccgttcatt
    36361 cttatccacc acaccctcac atttcagacg tcgctctaaa ggacaggctc ctagagaaat
    36421 taaaaagaaa tgtccattgg gttaatgcca agtatcctgt atatgaatag gtcattctga
    36481 ttaaaaaatg tgtgaatacc attcataacg aaaagggatt gggagacgcc gatgaaaagg
    36541 aatttactcc atg
//
LOCUS       DAORAQ010000013        35690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_255_length_35690_cov_67.459885, whole genome shotgun sequence.
ACCESSION   DAORAQ010000013 DAORAQ010000000
VERSION     DAORAQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 35690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35690
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_255_length_35690_cov_67.459885"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PKM72_02485"
     CDS             <1..1101
                     /locus_tag="PKM72_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP59676.1"
                     /translation="RELYLFGNVSIIRDEQGQVGGAVGTFTDLTSFILNNQKIAVLEE
                     QAKSRESFQQLIGKSPAMQEVFRRLRLAAQSDVTVLLTGESGTGKELAARAIHTLSDR
                     KDKPFFAINCSAIPETLLESELFGHVKGAFTGAIRDKIGVFQAAGGGTLFLDEIGDTS
                     PLLQLKLLRVLQESEIRRVGDDRGMQVDVRLITATNRDLKVLMAEGTVREDFYYRIHV
                     FEITLPPLRARREDIPLLVHHFMAASSMTFRREIGDIAQDALQRLIDYNWPGNVRELK
                     NAIEHAFVTVNGDCLTLWDLPSEVQRPPRSKSLKPLSSQVEPSLPPDEEARIAEALQQ
                     TKGNKTEAAKLLGVSRVTLWKKIKQLQSPPHA"
     gene            1369..2799
                     /locus_tag="PKM72_02490"
     CDS             1369..2799
                     /locus_tag="PKM72_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP59677.1"
                     /translation="MIVKQFYLSCLAHASYMIGDHKTSTAVVVDPQRDVEQYLQEARR
                     HDWKIRYVFLTHFHADFLAGHLELQRQTGAEICLGAKAKTDFPIRNFRDKEVLEFGSV
                     RIQVLETPGHTPESISLAVYDLSRSADHPHCVLTGDTLFIGDVGRPDLMASIGVTAEE
                     LGGQLFDSLRNTLMRLPDETLVYPAHGAGSMCGKNLSSDTVSTLGRQRWENYALQPMT
                     KEKFVELVTADQPEAPSYFGYDAKMNREQHPVLDDVVRNSLTPLSLQTVLDHQKSGTQ
                     VLDVRNAVEYAGGHLKGSLNIGLGGKFATWAGTVLDPKRSIVIIAEPGHEKEAIQRLG
                     RIGFDRVLGYLKGGMQSLEVNPDIIQKVQRISAQGLAELLTTSQPPMVVDVRSEKEWQ
                     AGHIDRSINIPLPHLAEHLQDIPADAPVVVHCASGYRSSTAIGILEQAGRTQAMDLIG
                     GHDAWLTTWGHPRHKVQHENTAACAK"
     gene            2819..3406
                     /locus_tag="PKM72_02495"
     CDS             2819..3406
                     /locus_tag="PKM72_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNP59678.1"
                     /translation="MNMMLLDQKPKSGMGHKNQVSPLSLNDPIYIDVRTPQEFEGMHI
                     SGSRNIPLPDLHTYVEELQTLSREHPIRLLCRTQNRVKIAYEYLTNHGLTNCEILQGG
                     ITAWVNQGKPVVRGQQRISLEGQVRAIAGGLILAGVAFGFTVHSGFFFIPALVGAGLL
                     HAGLTDSCLMGMLLAKLPMNRIENSSKNIGKKGDS"
     gene            3403..3627
                     /locus_tag="PKM72_02500"
     CDS             3403..3627
                     /locus_tag="PKM72_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59679.1"
                     /translation="MMNCPRCEGLLMSDQVYNPEEAVYVLPIWRCVNCGESFDPMILQ
                     NRTTQKAKGIMQHSKSTSWTNNRTPLAITR"
     gene            3704..4222
                     /locus_tag="PKM72_02505"
     CDS             3704..4222
                     /locus_tag="PKM72_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HNP59680.1"
                     /translation="MHHLIRSVLMLGGFSLLAMGWMTTPAMSEGKSPTDGYDIHIQAP
                     HMMADGTVGGPYHHYCKGISDELLQCQLYESTDPNAKLVAIEYFIAKDLARKNVPLIQ
                     WNRAFHDHQVEIDTGRVQILDVEDPAKVKALAEAAGKTDGVIFHLWGKHQVVPDGAVS
                     IPTSLGHVFRTE"
     gene            4295..4762
                     /locus_tag="PKM72_02510"
     CDS             4295..4762
                     /locus_tag="PKM72_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNP59681.1"
                     /translation="MIRRKLGWLLVGYFIFQILWAFSSEAADLEVLKPRVPQDQIAEA
                     RSWENPFPSTPENIKKGKHIFHGKAFCVTCHGPDGKGLGDIPGLRGKLPRNFTDKTWQ
                     AARTDGELFWILQNGSQGTAMASFIPLVLTEEEAWYVILYVRSFGNGVESRKE"
     gene            5094..5501
                     /locus_tag="PKM72_02515"
     CDS             5094..5501
                     /locus_tag="PKM72_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59682.1"
                     /translation="MTVMPLVVLATWLAFTSVESPSPSTTLALPIEPDESTRIDEIYD
                     QEALMLLSLYSLKGNGKADYITGRTVERHVRSTYGNPVYYTLEFPVFYWWNHTMWNDP
                     FVDGVNGNEQVYQEETDFDVSRYKPCVFNGQTC"
     gene            5799..7031
                     /locus_tag="PKM72_02520"
     CDS             5799..7031
                     /locus_tag="PKM72_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNP59683.1"
                     /translation="MNEKKPGIDRRQLLQGSLALGLTGLLTPKGLWAKDSPTVTLPFE
                     RGRRPLVAFPQKRPLMVMTTRPPQLETPFHIFNEDIFTPNDAFFVRWHLANIPQKIDM
                     NGFRLTIRGRVNHPLSLSLHELQTQFEQVEIAAVAQCAGNSRGFFQPRVPGGQWGNGA
                     MGNANWKGIRLRDLLLKAGIEGDAVQVRFDGLDQPVAQATPDLRKSLRLDDALQEEVL
                     IAHSMNGEALPMLNGFPLRLVAPGWYATYWVKMLNDIEVLNEADQNFWTTQAYRLPAN
                     PCHCVKPGEALTDTVPIGKMPVRSFITNLEEGRTIPGGESCTIKGIAFDQGYGIDRVL
                     VSVDGGQQWLSARLGKDYGDFSFRPWEAKFIPKPGQAYALQSLAINRIGESQRFGARW
                     NPNGYLRNVIETVNIQAS"
     gene            7044..7709
                     /locus_tag="PKM72_02525"
     CDS             7044..7709
                     /locus_tag="PKM72_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNP59684.1"
                     /translation="MRVCMVVVVFLWISGLAWELPANGENRDQQVSILNLPPDPISFQ
                     PGSGSELASRYCLMCHSAEYVYMQPPHPHGQWMEIVNKMKESFGCPIPNEDMAQLVDY
                     LVTQNSLQPSPSAPPAQRDDQTDTPGQSSIQSGDSQKGKSLYERHCVTCHGPGGKGDG
                     PIGPVLVPPAANLTLLGKQSDKTILDTLRKGRPGTAMPAWEHDLNPQELNHLLSFLRT
                     LTP"
     gene            7924..9354
                     /gene="pyk"
                     /locus_tag="PKM72_02530"
     CDS             7924..9354
                     /gene="pyk"
                     /locus_tag="PKM72_02530"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNP59685.1"
                     /translation="MRHVKIVATIGPATASQECLKQLLHAGINVARFNMSHGTAEWHK
                     TTIQRLRCLALEEKTPLAILVDLAGPKIRLGKLPDEGTLLQKGRDIILVAKSDQSSRQ
                     NESEETLPVNLSHFPDRMEPGQIVLIDDGNLALTLEKQEPNRLICKVLVGGKVTSHKG
                     VNFPGLPLDIPGFTKKDADDLQVAIDIEADYIALSFVRSPQDILTLQRALADRSAVIP
                     LIAKIERPEALTCLDEILDASDGVMVARGDLALEMSPEEVPLLQKQIIAQANRMGKPV
                     ITATQMLESMTRNAFPTRAEASDVANAVLDGTDAVMLSAETSMGNFPLEAVQVMDRII
                     RQAEKEMFRKSQNLHSPKGVVRSTPEAVCEAAVSLAKSINAQAIVVFTESGHTAMLLA
                     RHRPTVKIFALTPSPTVTRQLALVWGMTSSVFPQINKTDDRIHAAQDHLKTLGLLQES
                     RLIVIVTGEQLGNAFGTNIIKAHVVV"
     gene            9379..11031
                     /locus_tag="PKM72_02535"
     CDS             9379..11031
                     /locus_tag="PKM72_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP59686.1"
                     /translation="MSLNIIIIGGVAGGASAAAKARRTNEAANIEMFEKGPYVSFANC
                     GLPFYVGETISDRDDLLLQTPERFWKRFRVQAYVLHEVLHIDRTAKCVQVKNLMTQAI
                     TTHPYDTLILAPGAGAIMPDIPGIHARNIFTVKTVPDSDAIKTFLAGQPSQHALVIGG
                     GFIGLESAEALIGLGLTVTIVEKAPQILPAFDQDMATLVAHHLQEKGVKIIAGDGING
                     FRQDGDLAKEAELESGTRLPMDLAILSIGVRPELKLAREAGLEIGAAGGIAVDTRQQT
                     SDPDIYAAGDAVETLHLVTGKYARIPLAGPANKQGRVAGANAAGGDLRFHGALGTAIV
                     ESMGITAAKTGLSEHEAKAHGLNYFVSLTHPLDHAEYYPGAVALHMKLVVEQQTGRLL
                     GAQIVGDQGVDKRIDVLATAIHGNLNVQDLEQLDLAYAPQFNSAKGPVIMAGFVAANT
                     LRGEVNTLTGEELQQKIKTNPSLQLLDVRTTDEYQEAHIPHSRLIPVDELRDHLQQLD
                     PSQETVVYCRVGLRGYLAARILLQHGFTKVFNLTGGFLSFPQ"
     gene            11292..11738
                     /locus_tag="PKM72_02540"
     CDS             11292..11738
                     /locus_tag="PKM72_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59687.1"
                     /translation="MEQTTSKSRTAAMFSGQATLGRILVVDDEPDVRKVVKLSLEKAG
                     YEVIEAEDGQQAVQEIKSGENPLLLDVILTDIRMPKLNGLEAIQFFQKEFPRVSLIVL
                     TGFPDLTMATSLMKNGVIDYLVKPVEKEKLLTAVAKAMEQRELNHL"
     gene            12023..13456
                     /locus_tag="PKM72_02545"
     CDS             12023..13456
                     /locus_tag="PKM72_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP59688.1"
                     /translation="MAHTRIVHFRTQGHALVLTVGVLLFLLFPGPLFAADGKDAEALI
                     NTTCSTCHKFQGEGESRFHLKAPDLMWGGSKFQRDWLIKWLTGKEPMLYAKSYRWDQS
                     QQPEQHMTVSEPEAEGLADYFETHLQDPRVKPGSIDMSTFSKQEAKFGEEIFTQHSCI
                     GCHQIMVDGKKTGGPQSASFFNSGKRLKADWIYRFNSDPPDFVPHSGEFVGDVSALGL
                     RYVTGFIATRGNEDFPYYEPWKSADFDHPNMENGAKIYQEYCAQCHGAEGKGDGPAAS
                     GLEPKPAVHANIPFEKIPLDYLYNVIYYGGKAVGKSPLMPYWGLTIGGTEGVSDVIAY
                     LKTTFKGAPEMAGTASPSGGTSGVCPQPRNTKRAPGKFRNMTSPLPNSQEHITAGEKL
                     YHETAQPLACTQCHGDKGDGQGAMGAALNPHPRNFTCGETMKDISDGQLYWIIKNGSP
                     GTGMMAFSGMPDNQIWQVIQYIRTLAK"
     gene            13558..15168
                     /locus_tag="PKM72_02550"
     CDS             13558..15168
                     /locus_tag="PKM72_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP59689.1"
                     /translation="MPPAPENVQQDNSDLPRMLKHLEQHHALILQAAGEGIFGLDLEG
                     RHTFVNQAAAKMLGYEPQELIGQPSHSLWHHTKADGTPYPQEQCPNYATYKDGQVHRG
                     DNEVFWRKNGTSFPVEYSSTPIRHEGVLVGAVVVFQDITYRRQCEKSLEQFRRHNDMI
                     LQAAGEGIFGLDLQGHHTFVNPAANILLGYEPGELFGKPSHTTWHHTKSDGSPYPAEE
                     CPIYGAYKDGLVHQGEEEVFWKKDGTSFPARYTSTPIWDEQGGLAGAVVTFQDITEKK
                     RMAAQLLEEAKLAEVTRVLGDIGHDIKNMLMPVLSGADLLKDELDEQFPTLIREKAKG
                     AETSYANSLDLIRMIVTNAKRIQDRVREIADAVKGVTSPPHFTPCRIRDVVDGVLDTL
                     RTYAGEKGITLLTDGLDALPVIDADERRLFNAFYNLINNAIPEVPRGGSVTVGGSPGP
                     RPDTVELTVADTGKGMPAEVRDRLFTAQAISTKKEGTGLGTKIIKDAIDLHHGLITVE
                     SEEGHGTIFRLVLPVSQVVSSQAKPGNT"
     gene            16050..16838
                     /locus_tag="PKM72_02555"
     CDS             16050..16838
                     /locus_tag="PKM72_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNP59690.1"
                     /translation="MDFHYLLALLSGGVIGLLLGATGGGGSLVAIPLLVYVVGIPVQN
                     ATAMSLIVVGYSALFGAWHESRQQRVHFISALLFSLTGMVGAWVGAHGHQLVPDSLVL
                     FSFGNLLLFISMWTFWKNYEGEQNDVPSGCAKEFSGRCALKALTLGWGVGLLTGFFGV
                     GGGFLIVPALMFLMGFPIRLAIGTSLLIIALISIGGVIGHFREAHLDIVLTGLVLLGS
                     LLGLMFASQVTRSIPAHHLRRGVAILIGVIGILLIVANARPLLF"
     gene            complement(16857..17930)
                     /locus_tag="PKM72_02560"
     CDS             complement(16857..17930)
                     /locus_tag="PKM72_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNP59691.1"
                     /translation="MAFLFRTPASRKASRLFFRRVVYMPTDLDPFRTLLSRIILVAML
                     FCFLTGILWLDRNGLKDQIDGEITFSDVIYFTMVTVTTVGYGDIVPVTTRARLIDAFV
                     VTPVRIFLWVIFLGTAYQLAFRQFTEVFRMAKLQRSLDQHVVICGFGHTGYSTVKELL
                     AKGTNPDHILVIDPGEERVRVAGELGVVALRGDATQEGILKYGAFLNEAKAVIIAAGR
                     DDTNALILLTVRNLNSRCRVVVSAKEEENVKLFRQGGAQTIISPATYGGYMLAAAVDQ
                     HFLVDYLEDFLTAGGKVRIEERTADQQDVGKSAMDLRPDLLLRVYRQGTILSHWEFEK
                     NHTLELGDVMLLFKPVSEDAASS"
     gene            complement(17964..19784)
                     /locus_tag="PKM72_02565"
     CDS             complement(17964..19784)
                     /locus_tag="PKM72_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNP59692.1"
                     /translation="MNSDHLFFLGLMGIMLVFFIWERWRYDLVALSALLLSTLLGFVP
                     SEEAFLGFGHPAVVTVAAVLIISRGLSNAGAVEILTRYVNVATSSSPSIHVAVLSSLG
                     GLISTVMNNVGALALLMPVAIQSSVEAKRSPAVVLMPLAFGTILGGMVTLIGTPPNII
                     VAHYRAEVAGEPFGMFDFTPVGGIMALVGILFVALVGWRFIPIARRSQNAPQDLFSIQ
                     EYLTEVEVPENSKAIGKSLSELEMATEDSDALIIGLVRGDQSIRGAAWREHIQVGDVL
                     VLEAGPQGINKFVSTLGLKLTGTQPKEEIQDSKKAPPAKRDEDMLLEAVVPPDRSWLV
                     GRLAGSLKLRSRFGINLLGASRQGTPYRGRLREFRFKAGDVLLLQGEKERVLEVIGQL
                     GCLPLAERGLQLGKPGQAWLAGGLFALALGSAMSGLASLPIALICSALGMVLLNIVSP
                     RDVYQSVDWPVLVLLGAMIPIGQALERSGATTVLAQVLVNMMEGFPPIMLLGIIMVLT
                     MTLSDVMNNAATAVVMAPLSVAVAQNLGVNTDPFLMAVAIGASCAFLTPIGHQNNLLV
                     MGPGGYRFGDYWRMGLPLELLILLVSIPLLVFMWPLTGQS"
     gene            complement(20366..20596)
                     /locus_tag="PKM72_02570"
     CDS             complement(20366..20596)
                     /locus_tag="PKM72_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59693.1"
                     /translation="MTCVRCNGLMVVDDCLDIKGAMGELWIKAYRCIMCGNLMDPVIN
                     HHRTDGPVQAQVIPFRKRLRRAPRTPVARLTA"
     gene            complement(20974..22041)
                     /locus_tag="PKM72_02575"
     CDS             complement(20974..22041)
                     /locus_tag="PKM72_02575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNP59694.1"
                     /translation="MNRIDTELGNALKGKIRTQGPITFRDFMAAALYDENMGFYAKAP
                     KIGSPDGPFDTNARFPAFGYAIAKAITYAEKAVDVPLRVLELGGGTGQLGSHIIACLE
                     NDHEYVVLEPSQGLRANQQQRGLQAIQNIESLPPKPTFVFGNEVLDALPVHRVMGMGH
                     EEVLELYVDLDEDGEFCEQPRPLSTMALAERLSGERVQLGRGQVAEICLELKPFLKSI
                     WKVVDPGYVIFIDYGDCAKNLYSHRHRNGTLRSYYHQKQIYDQFFAVGQQDLTADVDY
                     TAACFLAEEVGFEVDGLIPQGRWLRNLGILDYKGSAGDRESDQEEIDVLTRPAGLGST
                     FDVLIFKTKGLSNGPGLQPTS"
     gene            complement(22200..22553)
                     /locus_tag="PKM72_02580"
     CDS             complement(22200..22553)
                     /locus_tag="PKM72_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59695.1"
                     /translation="MKGIGIFLGTVGASTFALSMAFANPGLLPAHPGYPAAGKSPVTG
                     QATANDPGQTNAGGETSLLSSQGFGSKAAMNDVNDPNRARIQKSAGAGRLPEVEGPIN
                     KITPNPAGAKSTVIK"
     gene            complement(22735..24531)
                     /locus_tag="PKM72_02585"
     CDS             complement(22735..24531)
                     /locus_tag="PKM72_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59696.1"
                     /translation="MSNRLEPTLAAKQQSTLQKILTSVVNEVGMEAVLVAIMTHDGGP
                     LVEQVSRGFSPREVRAILRALSMEDLKSVAVRNGGGGELESVLRVRMVTPGSKVALVL
                     PLKFGGRVYGTLVLARRENSALTKREKTTLSTNVSHISTELKKAGLFGSSLILSKPFV
                     GNEPVSLAVEEGHATVARTYSTAEVQERIGSILTEDVEGGLSFDRGWVSIYDPLAATL
                     EVLGVFGTQKRDVTPGQLLSLDDSASGWSVRHRKPRCDNNLASTQGRFHDYKQLYRDR
                     FASTIVVPFYVRGRVAGTVTLASKNPNNFDQTGSDSKSLDAITTKLVELFEDPSCHLS
                     VMDVAPVQPEKPVLKGQVIVAPPEGGDVRKEERRAALHEVSSFLATEIREPIGFIRAQ
                     LEEITTDADLDFDSQTRVESAMRDMIRIETVLNEILDFAKPLELDRKMCRVQDLLDKA
                     FSLVSTDIRVNRIEVIKKVPARLAQVRWDEVKMQHVFLCIFKNAIEAMSPGGHLRVEV
                     MLTRARKPELQIIIANDGVPIPAEFVDKVFEPYFTTKRSGTGLGLATVKKVVEEHQGQ
                     ISIASEPDKGTTVTILMPAPRPRTPYRPRRPV"
     gene            complement(24677..25078)
                     /locus_tag="PKM72_02590"
     CDS             complement(24677..25078)
                     /locus_tag="PKM72_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNP59697.1"
                     /translation="MKGFLIRCGITGFAVLLASQIIPGIEITGVASGIAAVIILAFLN
                     AIIRPVLYLLSAPFIFVTLGLFMLLINGFLLYLVSILVKGFIISGFWPAVGGALIISL
                     VSGVLNLWISEQGRIEIVTHPSSGRKIRHIN"
     gene            complement(25160..25972)
                     /locus_tag="PKM72_02595"
     CDS             complement(25160..25972)
                     /locus_tag="PKM72_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNP59698.1"
                     /translation="MEYVYLLTNTFILRPYVFVFLGVSLYSGQQLLGWRRAGRFFGLT
                     WAIAFICEYASTRIGIPFGDYFYTGSTQDHELYLSNIPFMDSISFSFLLYSSYCLALI
                     FVLTPAKGVDQQGWLFDPELRNSWPVVGLTVVLFTFSDVVIDPVALQGERWFLGNIYG
                     YHQEGIYFGVPLANFAGWAVVGFLSLLGYRLMERGTNATDPIPRMVVKWELILGIGLY
                     YGVLAFNLGMTFWIGEMLMGIVGSFIFVPLTAVLLNTLWRGLFDYRVKESSE"
     gene            complement(26019..27005)
                     /locus_tag="PKM72_02600"
     CDS             complement(26019..27005)
                     /locus_tag="PKM72_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNP59699.1"
                     /translation="MKVLITGSSGFIGAAVTRAVVAKGDEVRVLVRPTSNLKNLEGLP
                     VETVYGDLQDYLSLKKAIAGCEGLYHVAAHYALWDQNPSVFYAINVEGTKNLFRAASE
                     AGVQRIVYTSTIGAIGLPDQRGLGNETLFPTLAQLAGDYKRSKFLAEQEVLRMAQEGL
                     PVVIVNPTAPVGERDVKPTPTGQIIVDFMRGRMLAYIETGMNLVDVEDVAIGHIRAME
                     CGRVGERYILGNQNLTLKEVCQILSRLTGVPAPRLRLPWRLVLPLAHLNQWIANLVTH
                     RSPRIPLEGVRMAKYHMHYDCTKARQELALPQTSVETALNKAVEWFRQHGYA"
     gene            complement(27107..27997)
                     /locus_tag="PKM72_02605"
     CDS             complement(27107..27997)
                     /locus_tag="PKM72_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNP59700.1"
                     /translation="MNIQAYLDQQRQRVDQFLERSVPLLSVHPQRLYESMRYSLLGGG
                     KRIRPILTIATAQALGYDRDAMLPFAASLEFVHTYSLIHDDLPAMDNDDYRRGRLTNH
                     KVFGDGMAILAGDALLTMAFELCSQVDGSEAFSAAQQLDIVRELAHGSGHRGMVGGQV
                     MDIQAENQEIDLPHLQQIHTCKTGRLIRAAVRIGGIIGEAKTQQMQCLTDYSEDIGLA
                     FQIADDVLDMVGTREELGKDAGTDERRGKQTYPSFFGVEGARQLGEECVKRAITRLDA
                     FDKQADPLRDIATYIMARRS"
     gene            28860..29039
                     /locus_tag="PKM72_02610"
     CDS             28860..29039
                     /locus_tag="PKM72_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59701.1"
                     /translation="MKNVELTVNGTMLTITVDLSKDFGPSSSGKTTIIASTEGNVSIP
                     DREEKIGLNIYKKKS"
     gene            complement(29046..31580)
                     /gene="uvrA"
                     /locus_tag="PKM72_02615"
     CDS             complement(29046..31580)
                     /gene="uvrA"
                     /locus_tag="PKM72_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNP59702.1"
                     /translation="MIYSPPMNNSIVIRGARQHNLKNLDLEFPRDQLVVITGLSGSGK
                     SSLAFDTIYAEGQRRYVESLSAYARQFLDQMTKPDVDSIEGLSPAISIEQKTTSHNPR
                     STVGTVTEIYDYFRLLFARIGQPFCYLCGNAIAAQTVQQMVDAIHVLPLGTKIHILAP
                     IVRGRKGEYRKELLAARKAGFVRARIDGAIRDLGESIHLHKQRKHTIEIVIDRLIVKS
                     EPGVSRRLADSVETALKLAHGLVGVLTEDDQVRVYSEHLACIQCGVSYSEISPRIFSF
                     NSPHGACDGCDGIGYEASTHSEWEEWTFDTPCEKCGGGRLKKESLAIKIGGQSIAELT
                     HLSVRNTLAFMNALNLTDREQMIGERVLKEIRERLEFLLNVGLGYLTLDRPSATLSGG
                     EGQRIRLATQIGSGLVGVLYILDEPSIGLHQRDHRRLLKTLLRLRDMGNTVVVVEHDA
                     ETMRAADYVLDLGPGAGVEGGELVAHGSPAVVMAHPTSLTGQYLNGNRRVSLPQRARH
                     PKGFLTVVGAEKHNLRDLTVNFPLGLLTCVTGVSGSGKSTLVVDVLFRSLGQGFSQKK
                     PVFEGCREIRGLDQLDKLIDIDQSPIGRTPRSNPATYTGLFSFIRDLFAQLPESRVRG
                     YKPGRYSFNVKGGRCEACQGDGLIKIEMHFLPDIYVTCEACNGQRYNRETLDVTYRGR
                     TIADVLNMTVAEALEFFVNIPPLRIRLQTLSDVGLHYIKLGQSATTLSGGEAQRVKLS
                     KELSKRPTGRTLYILDEPTTGLHFVDIQRLLDVLDRLVETGNTVLVIEHNVDVIQNAD
                     WVIDLGPEGGDQGGCVVAEGTPQAVMKVKESWTGQVLLEDSKSRAC"
     gene            31737..32294
                     /locus_tag="PKM72_02620"
     CDS             31737..32294
                     /locus_tag="PKM72_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59703.1"
                     /translation="MKNLKTACILTATIILTSLFSLAPPAWAAKYELTRNSIEDAKDV
                     DAGDISLFRVKLGDAEAIAVEALVEARISGVRAEQEGTFILLWDKENPTGAMAGVRIT
                     DGKVDLIFINNRFAHKVRGIFRHILGAKSTKEIRDLLGPEDFPNETLMGAKLLYEKQG
                     FLVNFLERQVNVEFCLGCQDLFSGF"
     gene            32458..33471
                     /gene="ruvB"
                     /locus_tag="PKM72_02625"
     CDS             32458..33471
                     /gene="ruvB"
                     /locus_tag="PKM72_02625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNP59704.1"
                     /translation="MDDRILTTHLIEEEQGLEGTLRPQRLTEYIGQERMKESLRVCID
                     AATGRGEALDHAIFYGPPGLGKTTIAHIIAKEMGGTIRSTSGLVLTHAGDLAAILANL
                     QPRDVLFIDEIHRLPPAAEEVLYPAMEDYQIDLVIGQGPSLRTMKLDLPPFTLIGATT
                     RAGSLTSPLRERFGLVYRLDYYLPEELQVIIVRSARLLGVHIDDEGAGEISCRARGTP
                     RIANRLIKRVRDFAEVKAQGRITRSVAQEALQWLGVDHAGFDEMDRKILLTIIKKFKG
                     GPVGIDALAAAIQEEKSTIEDVYEPFLLQSGYLDRTARGRQITSKALQHFGENPNLFS
                     MGI"
     gene            complement(34330..34851)
                     /locus_tag="PKM72_02630"
     CDS             complement(34330..34851)
                     /locus_tag="PKM72_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59705.1"
                     /translation="MNNDISNSGSGMKRRVWLKMFCALLMVSTLSACTGDRYQQQADV
                     IKGHVEMFYGHLQSHQPARALADNEQIEVLALRSEERLLRRVGQMRQGERTQEWKIIK
                     TAKETAAENWLALARYFSESQQYNKALGTYKRIIETYQGLAYQLYVDRAKTGMQDVEA
                     ILDSGTRPVGPAR"
BASE COUNT         9430 a         9264 c         8227 g         8769 t
ORIGIN      
        1 cgagaactct acctcttcgg caatgtctcg atcatccgag atgaacaagg tcaagtcgga
       61 ggtgctgtgg ggacctttac cgacctcact tcatttattt taaataatca aaaaattgcc
      121 gtgctggagg aacaggcgaa atcccgtgag tcctttcaac agctcatcgg caaaagccct
      181 gcaatgcaag aagtgtttcg ccgcctccgc ctggccgcac aaagcgatgt cacggtgctg
      241 ctcaccgggg aatctggtac cggaaaagaa ctggcagccc gagccattca caccctgagt
      301 gatcgaaaag acaaaccttt tttcgccatt aattgctcag ccattcctga aaccttgctt
      361 gaaagcgaac ttttcgggca tgtgaaggga gcatttaccg gggccatacg cgataaaatc
      421 ggcgtctttc aagccgccgg cggtggcacg ttatttttag atgaaatcgg agataccagc
      481 cctctcctgc agttgaaatt actcagagtc ctgcaggaaa gcgagattcg ccgggttgga
      541 gatgatcggg gcatgcaggt cgatgttcga ctcattacgg ccaccaatag agacttgaaa
      601 gtccttatgg cggaaggcac ggttcgggaa gatttttatt atcggattca tgtgttcgag
      661 attaccctcc cgcctttgcg agccagacga gaagatattc ctctactggt tcaccatttc
      721 atggcggcca gttccatgac gtttcgtcgc gaaataggag atatagccca ggatgctctt
      781 caaagattaa ttgactataa ctggcccgga aatgtccgcg aattaaaaaa tgcgatcgaa
      841 catgccttcg tcaccgtcaa tggtgattgc ctcaccctgt gggatcttcc ttccgaagtc
      901 caacgtcccc cacgatctaa aagtctcaaa cctctctctt cccaagtaga gccttccctt
      961 ccaccggatg aggaagctcg cattgctgaa gccctccaac agaccaaggg caataaaacg
     1021 gaggctgcca agctactcgg agtgagccgg gtcacccttt ggaagaaaat caaacagctt
     1081 cagtcccctc cacacgcata accaaaccgt taatacgtta acttaacagt taacgcgttc
     1141 tgttcagtgc atattaaatt taagaccacc accggattta acctgtcgta gtctttccat
     1201 tatccattgt ttttgtttga aattttcaaa tacggctaca gaaaaacaat ctggcacaat
     1261 ccatgcttaa ctatgttgca tggacagaat cttaaaagaa tatttcgaaa cgagctcagt
     1321 cgctttcgac caatgctcct tttcaagaag gaggggaggg tatcaatcat gattgtgaaa
     1381 caattttatt tgagttgttt agctcatgcg tcttacatga ttggcgatca caaaacctca
     1441 accgctgtgg tcgtagatcc tcaaagggac gtcgagcaat atcttcagga ggcccgacgt
     1501 cacgattgga agattcggta tgtgtttctt actcactttc atgcggactt tctagccggg
     1561 caccttgaac tgcaacgaca aacgggtgct gagatctgtc ttggcgccaa agccaaaacc
     1621 gacttcccca ttcgaaattt tcgggacaaa gaggtgcttg agtttggatc ggtgcgcatt
     1681 caggtgctgg aaaccccagg acatacccct gaaagtattt cacttgccgt ctacgacctg
     1741 agcaggagcg ccgatcatcc acactgtgtg ctgacaggag ataccctgtt tattggcgat
     1801 gtcggtaggc cggatcttat ggcctccatt ggcgtgactg ccgaagaatt agggggccaa
     1861 ttattcgatt cgctccgaaa tactctcatg cgccttccgg atgaaacgct ggtctatccg
     1921 gcacacggag cgggatctat gtgcgggaaa aatttaagca gtgacacggt gtccacattg
     1981 ggcagacaac gttgggaaaa ctatgcgctt caacctatga caaaagaaaa atttgtggaa
     2041 ttggtcacgg ccgaccaacc tgaagcccct tcgtactttg gatatgatgc caagatgaat
     2101 agggaacagc accccgttct ggatgatgtg gtacggaaca gccttacgcc actttctctt
     2161 caaacggtgt tggatcacca gaaaagtggg actcaagtcc ttgatgtccg aaatgccgtt
     2221 gaatatgccg gaggccacct gaaggggagt ctcaatattg gattaggagg aaaatttgcg
     2281 acatgggccg ggacggtcct ggaccccaaa cgctccatcg tcatcattgc cgagcctgga
     2341 catgaaaagg aggcgattca acgattaggc cgcattgggt ttgaccgggt cctgggctat
     2401 ctgaagggtg gaatgcaatc ccttgaggta aaccccgata ttatccaaaa agtccaacgc
     2461 atctctgctc agggacttgc agaactattg accacctccc aaccaccaat ggttgtcgac
     2521 gtcagatcgg aaaaagagtg gcaggcaggg catattgatc gaagcatcaa tatcccctta
     2581 cctcacctgg ccgaacatct tcaggacatc cctgcggatg cccctgtagt ggtccactgt
     2641 gccagtggtt atcgatcatc caccgccata gggattttgg aacaagccgg acgaacccag
     2701 gcgatggatc ttatcggcgg gcatgacgcc tggctcacca catgggggca tccacgccac
     2761 aaagttcaac acgaaaatac cgccgcatgc gcgaaatagc cgtaaaggac aatacaagat
     2821 gaatatgatg ctcttagatc aaaaaccaaa atcgggaatg ggtcacaaaa atcaggtatc
     2881 gcctctttcg ttgaacgacc ctatttatat tgatgtcagg actccgcaag aatttgaagg
     2941 tatgcacatt tcaggttctc gaaatattcc tctgccagac ctccatacct atgtagagga
     3001 actacagact ctctctcgtg aacaccccat caggttactg tgccgaacac agaaccgtgt
     3061 gaagattgcc tacgaatatt tgaccaacca tggtctgacg aattgtgaaa ttctgcaagg
     3121 aggcattacc gcctgggtta atcagggcaa gcctgttgtc cggggacaac aacgaatttc
     3181 acttgaagga caggttcgag ccattgcagg cgggttgatt ctggcagggg tagcctttgg
     3241 cttcacggtt cacagtggat tctttttcat ccccgccttg gttggagccg ggctcctgca
     3301 cgcgggatta accgattcgt gtttaatggg tatgctgtta gccaagctac ccatgaaccg
     3361 aattgaaaat tcctcgaaaa atataggcaa gaaaggagat tcatgatgaa ttgtccacga
     3421 tgtgaaggat tattgatgag tgatcaagtc tataacccgg aggaagctgt atatgtgttg
     3481 cccatatggc gatgcgttaa ttgcggcgaa tcgtttgatc ccatgattct ccaaaaccgg
     3541 acgacacaaa aagcgaaagg gatcatgcaa cattcaaaat ccacaagttg gacaaacaat
     3601 agaaccccat tagctattac acgataggac gtctatagtt ttgcccaggc cctaacctgg
     3661 aattcagtca ggtctgtcac accttaatca caaggaggtt gctatgcatc atttaattcg
     3721 ttccgttttg atgttggggg gttttagcct tttggccatg gggtggatga ccactccagc
     3781 catgagtgag ggaaaaagcc cgacagatgg ctatgatatt cacatccagg ccccgcatat
     3841 gatggcggat gggacagtgg gaggaccgta ccatcattac tgtaagggca tttcggatga
     3901 actgttgcaa tgtcagttat atgagtccac cgatcccaac gccaaactgg tggccattga
     3961 atattttatt gccaaagacc tcgcacgcaa aaatgtcccc ctgattcagt ggaatcgggc
     4021 ctttcatgat catcaagtcg aaatcgatac cggtcgcgtg caaattctcg atgttgaaga
     4081 ccctgccaaa gtcaaagcac tggcagaagc ggctgggaaa accgatggag tcatcttcca
     4141 cctctggggc aagcatcagg tggttccaga tggggccgtc tcgataccaa cctcgcttgg
     4201 tcacgtcttc cgaacagaat aatctaacct cacagggaga gcggtctgtc gctctccctg
     4261 tattcctaca aactcattat tttaaggagc gcttgtgata cgtcggaaat tgggatggct
     4321 cctggtgggg tatttcattt ttcaaattct ctgggctttt tcaagtgaag cggctgatct
     4381 tgaagtgtta aagcctcggg tacctcagga tcaaatagcc gaagcgcgat cgtgggaaaa
     4441 tccatttccc tccacccctg agaatattaa gaagggcaaa cacatttttc acggcaaagc
     4501 cttttgtgtc acctgtcatg gaccagacgg aaaaggcctg ggagatatac ctggattaag
     4561 aggaaaactt ccccggaatt ttacggataa aacctggcag gcagctcgca ccgatgggga
     4621 actcttttgg atattgcaaa acggcagcca ggggactgct atggcctcct tcatccccct
     4681 ggtgttgact gaagaagaag cttggtatgt gatattgtat gtgagatcat ttggcaatgg
     4741 ggtagaatcg agaaaagaat aaaacaatga tacggccatc gacctcagta agttttctat
     4801 cgctcaccac gaggcgtttg tggccattta ggttccgaat ccgttgaccg ggcaccttaa
     4861 gaatgtcaat atgcgaaaga ggccacgcct ggagtggcga tgatcaaagt gttacatgcc
     4921 ggaaaaggcg gtgaattgat aggtattacc cctctcatca atatcgggga gggaagtgaa
     4981 acagcggatc cgcatggcct tgcggttgtt caaccctaat ttgttccgta cgcgagcatg
     5041 gccttttcct gaggccatat tccgaaccac atctaggaag gaggacttgg cttatgacgg
     5101 ttatgcctct agttgttctt gcaacctggt tagcgttcac ctcggtcgaa tctccctccc
     5161 cctcaacgac cttagccttg cccatagaac ccgacgaaag tacgcgcatc gatgaaatct
     5221 acgatcagga agccctcatg ctgctgtccc tgtattcctt aaaagggaat gggaaagccg
     5281 attacattac gggacgaacg gtcgaacgac atgtccggag cacctacgga aatccggtgt
     5341 actacacgtt agagtttccc gttttttatt ggtggaacca tacgatgtgg aatgacccgt
     5401 ttgtcgatgg tgtcaacgga aacgaacagg tctatcagga ggaaaccgat tttgatgtga
     5461 gccgttataa gccttgtgtc tttaacgggc aaacttgcta gccaaacggc gatggcagca
     5521 gggtcttctc cctcaactat ccatcggttc gtcgataggt catctagggc tttagtcccc
     5581 cccaaaaaac tccatttcag atgaatattc tgcataagaa tcaacggcat gggattatta
     5641 aaatggaaaa ccaggaacgg accttatagc gctttctgga atatctgatg agggtagagg
     5701 ctaggtattt attctatccg tgcattcaga taaaggcctg gcctctagaa gggcaggcat
     5761 tctggtttct attggaaaaa gacaaggtag attgacctat gaatgaaaaa aaaccgggaa
     5821 tagaccgtcg ccaattattg cagggaagcc tggctcttgg attgacaggc ctactgactc
     5881 ccaaaggcct ttgggctaag gattccccga ccgtcaccct tcctttcgag cgaggtcgcc
     5941 gtcccttggt cgcttttcca cagaagcggc cactcatggt gatgaccaca agacctcccc
     6001 aattggaaac tccatttcat atttttaatg aggacatctt tacgccaaat gatgcgtttt
     6061 ttgttcgctg gcatcttgcc aatatccctc agaaaattga catgaacggc ttccggctga
     6121 ctattcgcgg tcgagtcaat cacccactat ccttaagcct tcatgaatta caaacccaat
     6181 ttgaacaagt tgaaattgcg gccgtcgccc aatgtgccgg aaatagtcgc gggttttttc
     6241 aaccacgggt acccggcgga caatggggaa atggggcgat gggaaatgcc aactggaagg
     6301 gaatcagact tcgtgatctc cttctcaagg caggaataga aggtgatgca gtacaggtga
     6361 ggttcgatgg gctggaccaa ccggtcgcac aagccacgcc tgacttacga aaatcgctga
     6421 ggcttgatga tgccctgcag gaagaggtgt tgatcgctca ctccatgaac ggggaggcac
     6481 tccccatgct gaacggcttt ccccttcgcc tcgtggcgcc tggatggtat gccacttatt
     6541 gggtgaagat gctgaatgat attgaagtgc tcaatgaggc cgatcaaaat ttttggacga
     6601 cccaagccta tcgcttgccg gctaatcctt gtcactgcgt gaaaccagga gaggccttaa
     6661 ccgataccgt gcccattggc aaaatgcccg ttcgctcctt tatcacgaat ctggaagagg
     6721 gtcgaaccat tccgggaggg gaatcttgca ccattaaagg catcgccttt gaccagggct
     6781 atggcattga tcgcgtatta gtctctgtgg atggaggaca acaatggcta tcagcgcgat
     6841 taggaaagga ttatggagat ttcagcttcc gtccctggga agcgaaattt attccgaaac
     6901 ctggacaggc gtatgcactt caatcattgg ccatcaatcg gattggagaa tcacagcgat
     6961 ttggtgcccg atggaatcca aacggatatc ttcgtaatgt gatcgaaaca gtgaacattc
     7021 aagccagctg aggacaaccc gtgatgcgtg tttgcatggt cgttgtagtg tttctgtgga
     7081 tttcaggact agcgtgggag cttccggcca atggcgaaaa cagggatcag caggtatcca
     7141 ttcttaatct tcctcctgat ccgatatcgt ttcaacccgg ttccgggagt gaactcgcca
     7201 gccggtactg tttgatgtgc cattcggcag agtatgtgta tatgcaacca cctcatcccc
     7261 atgggcaatg gatggaaatc gtcaacaaaa tgaaagaatc atttggctgt ccaatcccga
     7321 atgaggacat ggctcaattg gtcgattatt tggtgacgca aaattcactc cagccttcac
     7381 cttctgcgcc tcccgctcag cgggacgacc agacagacac acccggtcaa tcctccattc
     7441 aatctggcga ttcccaaaaa ggaaaatccc tttatgaacg acattgcgtc acctgccatg
     7501 gaccaggtgg caagggagac ggccccatcg gaccggtctt ggttcctcca gcagccaacc
     7561 tcaccttatt aggtaagcaa tcggacaaaa ccatccttga tacgctccga aaaggacgcc
     7621 cgggtacggc catgccagcc tgggaacacg atctgaatcc acaagagctg aatcatctcc
     7681 tatcatttct tcgaacctta acaccataac accgatgaat ccttcgagcc tgactcagag
     7741 gccattccat cagcaattaa atccattcct tggatttctc ttttcatgcg ggtgcttccc
     7801 gttcgtagct ccgtgagacc cgaaaacctc cggggaatat tgacggttgt tcccataaaa
     7861 tcttccgctt ctctcaccaa tgtctgttag cgtcacctta gccaatttgg agatcccata
     7921 cccatgcgcc atgtaaaaat tgtagccacg attggccctg ccactgcctc ccaagaatgc
     7981 ttgaaacaac tcctgcatgc cgggatcaat gtcgccaggt tcaatatgtc tcatggaaca
     8041 gcggaatggc acaaaaccac gatccaacga ctgcgatgcc ttgctcttga ggaaaagacg
     8101 ccactggcca tccttgtaga tttggcaggt ccaaaaatcc gcctggggaa actcccggac
     8161 gaaggaaccc tcttgcagaa aggccgggat attatactgg ttgcgaagtc cgatcaaagc
     8221 tcaaggcaaa acgaatcgga ggaaacgctg ccggtcaatc tttctcattt cccggatcga
     8281 atggagccag gtcaaatcgt cctcattgat gacggaaacc tggccctcac cctggaaaag
     8341 caagaaccca atcgactgat ctgcaaagtc ctggtgggag gcaaggtcac ctcacataaa
     8401 ggggtcaatt tccctggtct ccccctcgac attccggggt ttacgaagaa agacgccgac
     8461 gatcttcaag tagccatcga cattgaagcc gattatatcg ccctctcgtt tgttcgaagt
     8521 ccgcaggata ttctcacact ccaaagggct ttggctgatc gatcagcggt gattcctctc
     8581 attgccaaaa ttgaaaggcc tgaagccctg acatgtcttg atgaaatcct ggatgcgtca
     8641 gacggggtga tggtcgctag gggagacctg gccctggaaa tgagcccgga agaagttccg
     8701 cttctgcaaa agcagatcat cgcacaggcc aatcgtatgg ggaaacccgt gatcaccgcc
     8761 acacaaatgc tggaatcgat gactcgcaat gcttttccca ctcgcgcgga agcctcggat
     8821 gtggccaacg ccgtactgga cgggactgat gccgtcatgt tgtcggccga aacttcaatg
     8881 ggaaattttc ctttggaagc ggtccaggtc atggatcgga ttattcgaca agcagaaaaa
     8941 gaaatgttcc gcaaaagcca aaacctccat tcgcccaaag gagtcgtgcg ttctactccg
     9001 gaagcagtct gtgaagctgc cgtctctctt gcaaaatcca ttaatgccca agctatcgtg
     9061 gtcttcaccg agtccggcca tacagccatg ctattggccc gccatcgacc gacggtcaag
     9121 attttcgcat tgactccctc cccaaccgtc acccgtcaat tagctttagt atggggcatg
     9181 acttcaagtg tatttcctca aatcaataaa accgatgacc gaattcatgc cgcccaggat
     9241 cacctcaaaa cgctcggcct acttcaggaa agccggctca ttgtcatcgt caccggagaa
     9301 caattgggaa atgcctttgg aaccaatatc atcaaagccc atgtggtcgt ctaaagtcga
     9361 gcaaggagaa taggaaacat gtccctcaac atcatcatta tcggaggcgt ggcaggcggg
     9421 gcgagcgcgg ccgccaaggc gcgtcggacc aacgaagccg caaacattga gatgtttgaa
     9481 aaagggccct atgtctcctt tgccaactgt ggtctcccgt tttatgtggg agagactatt
     9541 tcggatcgtg atgaccttct ccttcaaacc ccggagcggt tttggaaacg tttcagggtg
     9601 caagcctacg tgttacacga agtccttcac atagaccgaa cggcgaaatg cgtacaggtg
     9661 aaaaacctga tgactcaggc aatcaccacg cacccctatg acacactcat tctggcaccc
     9721 ggtgccgggg ccatcatgcc cgacataccc ggcattcacg ccaggaatat ctttacggtc
     9781 aaaaccgttc ctgactctga tgccatcaaa acctttctcg ctggccaacc ttctcagcac
     9841 gccttggtga ttggaggcgg atttattgga ctggaatcgg ctgaggccct gataggcctt
     9901 ggattgaccg tgacgattgt cgaaaaagct cctcaaatac ttcctgcttt tgatcaggac
     9961 atggccaccc tggtggctca tcatctccaa gaaaaggggg tgaagattat tgcgggtgac
    10021 ggtatcaacg gatttcggca ggacggcgat ctcgccaaag aagccgaatt agaaagtgga
    10081 acccggctgc cgatggacct ggccattctc tcgatcggcg tcagaccgga actcaagctt
    10141 gccagagagg caggacttga aattggtgca gcaggaggca ttgccgtgga tacacgacag
    10201 caaacctctg accccgatat ctatgcagcc ggtgacgcgg tcgaaacgct ccatctcgtg
    10261 actggcaaat atgccagaat tcctttagcc ggccccgcca ataagcaggg gagggtcgcc
    10321 ggagccaatg ctgcgggcgg agacttacgg tttcacggtg ccctcggaac ggcaattgtc
    10381 gagagcatgg gcatcaccgc tgccaaaacg ggattaagcg aacacgaagc caaggcccac
    10441 ggcttgaatt actttgtctc cctcacccac cccctggatc atgcagaata ttatcctgga
    10501 gcagtagcgc ttcacatgaa attagtcgta gagcaacaga cgggaagatt gttaggtgct
    10561 caaattgtgg gggatcaggg agtggataaa cgcattgatg tattggccac tgcgatacat
    10621 ggaaatttaa atgtccagga tttggaacaa ttggatttgg cctatgcccc ccaatttaac
    10681 tcggccaaag ggccggtcat tatggcaggt ttcgtcgccg ccaatacgct acgtggtgag
    10741 gtcaacacgc tcacgggaga agaattacaa cagaaaataa agacgaatcc ttctctccaa
    10801 ttgttagatg tccgaaccac ggacgaatat caagaagcgc atatccctca ttcccgcctc
    10861 attcccgtgg atgagctgag agaccatctt cagcaactgg atccctccca ggaaaccgtc
    10921 gtctattgtc gagtcggatt acgcggatac cttgctgcgc gcattctcct tcagcatggc
    10981 ttcaccaagg tcttcaacct cacgggcggc tttttaagtt tccctcaata aaattggatc
    11041 cccctaccat tcaagcaaac ccataaatct tcgaaacctt gagctgttaa ctcgttaact
    11101 caattcgtta aatcaagctt aacgcttcac cgcttttttc gttgctggat attttttcaa
    11161 acgtcataag caattgatat tccttatttt tatatcattt atgtcatttg gcatttggtt
    11221 tgctgatgtt tatgacatga agagaagaaa tccaaaggtc ccttaacaat tttcaccaaa
    11281 ggagagcgct catggaacaa acgacaagta aatctcgaac tgcagcgatg ttcagtggtc
    11341 aagccacatt gggccggatc ctggtcgttg acgacgagcc ggatgtacgc aaagttgtga
    11401 aattgtcatt ggaaaaagcc ggctacgagg tgattgaagc cgaagacggt caacaggccg
    11461 tgcaagaaat caaaagtgga gaaaaccctc ttttactgga cgtgatcctc accgatatcc
    11521 gaatgcccaa actgaacgga cttgaggcca ttcagttttt tcagaaagaa ttcccacgtg
    11581 tctcgttgat tgtgctgacg ggatttccgg atttaactat ggccacgtcc ttgatgaaga
    11641 atggggttat tgactatttg gtcaaaccgg ttgaaaagga gaaacttctg actgcggttg
    11701 cgaaggccat ggaacaacgg gagctcaacc atctataaga agttagaaga gaggggtact
    11761 acctccctct ttttgacgaa aaattctttc tgaaaaccta tgaggaaact tctagctttt
    11821 cattttggtg ttgattttgc tctatctatt aggaaagtgc tgaacatggg ttttatgagt
    11881 caaaatgccc catttaagat aagcttggga tggggaattt ttccccatag catattcaaa
    11941 aaagatcttt taaatcaaaa aacctcgaac atgatgatat tgtctggtct ttcttaacca
    12001 attattaatg gaggtgttcc caatggctca cacgaggatt gtccacttca gaactcaagg
    12061 ccatgctctc gtactcacgg tgggcgtcct tctctttctc ctttttcccg gtcctctttt
    12121 tgccgccgac ggaaaagatg cagaagccct gatcaacacc acatgttcga cctgtcataa
    12181 gtttcagggc gagggggaga gccgctttca tttaaaggct ccggatctca tgtggggggg
    12241 cagcaagttt cagcgggatt ggctgattaa atggctgaca ggaaaagaac caatgttata
    12301 cgccaaaagc taccggtggg atcagtctca acaacctgag cagcacatga ccgtctctga
    12361 accggaagct gaaggccttg ccgactattt cgagacacac cttcaggatc cgagagtaaa
    12421 gccggggtcc attgatatgt ccaccttcag caaacaggaa gcgaagtttg gagaggaaat
    12481 ctttacccaa cattcctgca ttggctgtca tcagattatg gtggatggga aaaagaccgg
    12541 cggtccacaa agcgcctcgt tctttaactc cggcaaaagg ctgaaggctg attggattta
    12601 tcgcttcaac tccgatcccc ccgactttgt gccacacagt ggagagtttg tcggggatgt
    12661 cagtgcactc gggctgcgat atgtcacggg gtttattgcg acccgaggga atgaggattt
    12721 cccctattat gaaccatgga aaagtgccga tttcgaccac ccgaatatgg aaaacggtgc
    12781 aaaaatttat caagaatact gcgcccaatg tcacggagcg gaaggcaaag gtgatggtcc
    12841 ggcggcttca ggcttagaac ccaaaccggc ggtgcatgcc aatattccct ttgaaaaaat
    12901 tcctctcgat tatctgtata acgtgattta ctatgggggg aaagctgttg ggaaatctcc
    12961 cctgatgccg tattggggac tgaccattgg cgggacagaa ggcgtatccg atgtcattgc
    13021 ctatttgaag accacgttta agggtgcccc ggaaatggcc ggcacggctt caccatccgg
    13081 aggaacatca ggagtctgtc cacaaccgcg caacaccaaa cgggcacctg gcaagtttcg
    13141 caatatgacc agtccgttac ccaactctca agagcacatc acagcgggag aaaagctgta
    13201 tcacgaaacc gctcaaccct tggcctgcac acaatgtcat ggcgacaagg gggacgggca
    13261 gggggcaatg ggcgcagcgt tgaatccaca tccccgaaac tttacatgtg gagaaacgat
    13321 gaaagatatt tctgacgggc aactgtattg gattatcaaa aacggatcgc cggggacagg
    13381 catgatggcg ttttccggga tgccagataa ccaaatctgg caagtgatcc agtacatccg
    13441 gactttggcc aaataaccgg aaattcggtt caaacccttc tgggtaacct gaaatttcat
    13501 ttcagggact ggagacacct tctccagtcc ctgaaccaaa cgagtaggaa gagcgccatg
    13561 ccaccagcac ccgaaaatgt acagcaggac aattccgacc ttccccgaat gttgaaacat
    13621 cttgaacaac accacgccct catcttacag gctgccggtg aaggcatatt cgggctggat
    13681 ttggagggcc ggcatacctt tgtgaaccag gcagccgcga aaatgttggg ctacgagccc
    13741 caagaactga tcggccaacc cagccactcc ctctggcacc acaccaaagc cgatggcact
    13801 ccctaccccc aggaacaatg tcccaactat gcgacttaca aggatggcca agtgcatcga
    13861 ggggacaacg aagtgttctg gcgaaagaat ggcaccagtt tccccgtcga atattccagc
    13921 acccctattc gccatgaagg ggttctggtc ggcgccgttg tggtttttca agatattacc
    13981 tatcgccgac aatgtgaaaa atcccttgaa caatttcgtc gacacaacga tatgatttta
    14041 caggccgccg gcgaaggcat tttcgggttg gacctccaag gacatcacac ctttgtcaat
    14101 cctgccgcca acatcttact cggctatgaa ccgggcgagt tatttggaaa acccagtcac
    14161 accacctggc accacacgaa atcagatggc tctccttatc ctgcagagga atgccccatc
    14221 tatggagcct ataaggatgg gttggttcac cagggtgagg aagaggtgtt ctggaaaaaa
    14281 gatgggacga gcttccctgc ccggtataca agcaccccga tctgggatga gcaaggggga
    14341 ttggcgggag ccgtggtcac gtttcaggat atcaccgaaa agaaacggat ggctgcccaa
    14401 ttattagaag aagccaaact tgcagaagtg accagagttc ttggggatat cggccacgat
    14461 attaaaaata tgttgatgcc tgtgttgtca ggtgcagatt tattaaagga tgaattggat
    14521 gaacagttcc ccaccttaat ccgggaaaag gcgaaagggg cagagaccag ttatgccaac
    14581 agcctggatc taatcagaat gatcgtcacc aatgccaagc ggattcagga ccgtgtgcgg
    14641 gaaattgcag atgccgtgaa aggtgtcacc agtcctccac atttcacacc ctgtcggata
    14701 cgagacgtcg tggatggagt gctcgacact cttcgcacgt acgctggaga aaaaggcatt
    14761 acccttctga ccgatggcct tgatgccctg ccggttattg atgccgatga acgtcgactg
    14821 tttaatgcct tttacaatct catcaataat gccataccgg aagtgccccg tggaggatca
    14881 gtcacggtcg gcggatctcc aggaccgcgt ccagacaccg tagaattgac ggtagccgat
    14941 accggaaaag gaatgcccgc tgaagttcgg gatcggctct ttaccgctca ggccattagc
    15001 accaaaaagg aaggaacagg gctcggaacc aaaattatca aagatgccat tgatctccat
    15061 cacggcctca ttacggttga aagtgaagaa ggccacggaa cgatctttcg tcttgtcctt
    15121 cccgtatctc aagtcgtttc atcccaggcc aagcctggca acacatgaca tcatcatgcg
    15181 attcaaagaa gattcattcc ttgcacaaat tgaatccctc ttagacaaaa ccatacattc
    15241 cccaaatggt aactgtcggt tgtatcgaaa tatctttgga ggatggtgtc cactgcgcgg
    15301 aaccactcat tgagtagtga tcctgacctg cccatccttg gaagaaatac ctttctcctt
    15361 tcctttaggt ccatggtcac ccccgaaacc acatcttctg tccgcgctag cctgttaatc
    15421 cttttcaggc aaccataaat caccatatat cattcgtaac ctgatcaaat attgcttagt
    15481 ctggatacat gacataaaaa cggggttgat ccagggaaaa acaattattg gttcccactc
    15541 acgtcaatta cccacccact tatgtctggg gaatcccatc ggacaagact tcgttttgta
    15601 atatagacgg gctgttaccc ataactggtg cccctgtagc caccggtaga aaaaagaatt
    15661 tcctaatggt tcgaccaatc cataaaaaca agaatctaag agccggttgc cctgatcaga
    15721 gcagactctt gtctcattcg ctttctcccc atacaattaa cttctactca ccttcaaacg
    15781 tttcatttgt tcatccctgg gagatttcct gaaagtaatc tatggggtat tgtccgcacc
    15841 gcctcgataa atcttacggc ctgcatgaat acctctgggc atcctggcct cagcgcatga
    15901 agtgggacag ctcctggaac tggaggcgat gaatatcatt gtggttcata atttcttcct
    15961 tagtccgttt ggcttttaaa ggtatgtacc acgattctga catctcaggc cctacccttt
    16021 cactttttcc tcttgcatct taggttctca tggactttca ttatttgtta gcactcctct
    16081 ctggtggcgt gatcggcctg ttattagggg ccacaggagg gggaggatcg ttagtggcca
    16141 ttcccttatt ggtctatgtg gttggcatac ctgttcaaaa tgccacagcc atgtcactta
    16201 tcgtggttgg ctattcggct ctctttgggg catggcatga gagccgacag cagcgggttc
    16261 acttcatctc tgccctttta ttcagcctga ccggaatggt cggcgcttgg gtgggcgctc
    16321 atggccacca gctcgttcca gactccctgg tgcttttttc gtttggcaac ctacttttgt
    16381 ttattagcat gtggaccttt tggaaaaatt atgaggggga acagaacgat gtcccgagcg
    16441 gatgcgcaaa agaattttcc gggcgttgtg cgctgaaagc cctcacgctc ggatgggggg
    16501 ttggcctgtt gacagggttt tttggggtcg gaggcggatt tctcattgtt cctgctttga
    16561 tgtttctcat gggatttcct attcggttag ctattggcac ctcgcttctc attattgctc
    16621 ttatttccat tggtggagtg atcggccatt tcagggaggc ccacctggat attgtgttaa
    16681 cgggattggt gttactcggc agtcttctag ggttgatgtt tgcttcacaa gtgacgcgat
    16741 ctattccagc acatcatctg agacggggag tggccatcct cataggtgta atcggcatat
    16801 tgttaatcgt ggccaatgcc agacctctcc tcttttaaaa atgggatgga gtgccttcaa
    16861 gaagaagctg catcctccga aaccggttta aacaatagca tcacatcccc cagctccagt
    16921 gtgtggttct tttcaaactc ccagtgtgaa agtatcgtac cttgccgata cacccgaagc
    16981 agcaaatccg gccgcaaatc cattgcggac tttccaacat cctgctggtc ggccgttcgt
    17041 tcttcgatac ggaccttccc gcctgccgtt aagaaatctt ccaaataatc caccaaaaaa
    17101 tgttgatcca ctgcggcggc taacatatat ccaccatacg tcgccggcga aataatggtc
    17161 tgtgcccctc cctggcgaaa gagtttgacg ttttcttctt ccttggcact gacaaccacc
    17221 cgacaacgtg aattcaaatt cctgacggtt aacaagataa gggcattcgt atcatctcgt
    17281 ccggcggcaa taatgacggc cttggcctca ttgagaaacg ccccatactt taagattccc
    17341 tcctgcgtcg catcccctct gagtgccacg actcccaatt ctccagcgac gcgtacccgt
    17401 tcttctcctg gatcaatgac caaaatatgg tctgggttcg tccccttggc gagcagttcc
    17461 ttgacggtcg aatagccagt atgaccgaat ccgcaaatga cgacatgctg atcaagagac
    17521 cgttggagtt ttgccatgcg aaatacctca gtaaattgtc tgaacgccag ttgataggcc
    17581 gtccctaaaa aaatgaccca aagaaagatg cggaccggtg tgacgacgaa tgcatcaatc
    17641 aggcgtgccc gtgtggtaac cggcacaatg tccccataac cgacagtcgt cacggtgacc
    17701 atcgtgaaat agatcacatc tgaaaaagtt atctctccat caatttgatc cttgagaccg
    17761 ttgcgatcca accacaggat ccctgtcaaa aaacaaaata acatggctac caatataatc
    17821 cggctcagaa gcgtgcgaaa gggatcaagg tcggtgggca tgtacaccac ccgtcgaaaa
    17881 aagagcctgg aggcttttcg ggaagcaggg gtgcggaata aaaatgccat gctgtcttct
    17941 tctcgtccct attcaaagat gcgttaactt tgccccgtta agggccacat aaagacaagc
    18001 agggggattg agaccaggag aattaagagt tccaaaggca accccatacg ccagtagtcc
    18061 ccaaatcgat acccccccgg ccccatgacc aacaaattat tttgatggcc aattggagtc
    18121 aaaaaggcac aagaggctcc aatcgccact gccatgagaa agggatcggt attcacccca
    18181 agattctgcg ccaccgccac gctcaatggt gccatcacga ccgccgtcgc ggcattattc
    18241 atgacatctg acaacgtcat ggttaatacc attatgatgc ctaagagcat aattggggga
    18301 aacccctcca tcatattgac caagacttga gctaacacgg tcgttgctcc actccgttcc
    18361 aacgcttgcc caataggaat catggccccc aacaagacta ggaccggcca atccacggat
    18421 tgatacacat ccctcggtga aacaatgttg agaagcacca tgcctaaggc cgaacagatt
    18481 aaggcaatcg ggagtgacgc caacccggac atggcgctgc ccaatgcaag agcgaatagc
    18541 ccgcctgcca gccacgcctg tcccggcttt cctaattgaa gtccccgttc tgccaaaggg
    18601 agacatccga gctggccaat cacttctaaa accctttcct tttctccctg cagcagaagc
    18661 acatcccccg ctttaaaccg aaattccctc agccgcccac ggtaaggtgt accttgacga
    18721 gaggccccta acaagttaat tccgaaccgg cttcgcaact tgagagaccc cgccagccgc
    18781 ccaaccaacc aggaacgatc cggaggcacc acggcctcca ataacatatc ttcatccctt
    18841 tttgcgggtg gcgctttttt agaatcctga atttcttcct ttggctgggt ccccgttaac
    18901 ttcaaaccca atgtggacac aaatttatta atgccttgtg gtcccgcctc caggacgagg
    18961 acatcgccga cctgaatgtg ttccctccaa gccgcccccc gaatagattg gtcacctcgc
    19021 accaatccaa taattaaggc gtcggaatcc tcagtggcca tttccagttc tgacaaagac
    19081 tttcctattg cctttgaatt ttccggcact tcaacttctg ttaaatattc ctgtatagaa
    19141 aacaggtcct gtggcgcatt ttgagatcgt cgggcaatgg gaataaagcg ccaacccact
    19201 aacgccacaa agagaattcc caccagcgcc atgattcctc ccaccggcgt gaaatcgaac
    19261 attccaaatg gctccccggc cacctctgcc cgataatggg ccacgatgat attgggaggt
    19321 gttccaataa gcgtaaccat tccacccaaa atcgttccaa aagccagggg cattaatact
    19381 acggccggag aacgcttggc ctcgacggac gactgaatag ccactggcat cagtaatgcc
    19441 agggcaccga cattattcat gacggttgaa atcaatccac ccagagaaga taaaacggcc
    19501 acatggatgg acggggaaga agacgtggca acattgacat agcgcgtgag aatttccacg
    19561 gctccggcat tactcaaccc acgactgata atcaacacag ccgccacggt gacgaccgca
    19621 ggatgaccaa atcctagaaa tgcctcttcg gaaggaacga accccaaaag tgttgagaga
    19681 agcaatgcac tgagggcgac caagtcgtaa cgccatcgtt cccagataaa gaacaccaac
    19741 atgattccca ttaggcccag gaaaaagaga tggtcactgt tcacgaacga gaagaatcct
    19801 tttattcagg gcatcgatga tgtggtcggg aaccggaagg aaattgccaa aacactccaa
    19861 gatcagatct taaaaaaact atgcaccctt atctgctccg atcattcgga gactaatgga
    19921 accctcttag tgtatcaagg ttgggaaagg aatgttccac catttcgatc cagcaggata
    19981 aaaatggtag ttacggacta gattggaatg ctcatgcagg ataccgggga tgcagggctg
    20041 aatgccgtcg cggaagtccc ccatccccat gctcagtcaa taattcaagg aggacttgtc
    20101 aggtaacctc aagcaatcac gccggaatag tcgcaggtga tagtatggta aaaggtcgag
    20161 gaatgtacca tgccaaagcc gtatggccag gaatgtttac ctctaaagga acccgcttca
    20221 gaaacgtcag aagaaccagg aaattaatct attagattga taggtcctcg ccgcaacaca
    20281 gcattcgaac ctcatcctga accatgaatc aatcggtccc tgggagcgtg gtccttccca
    20341 gaacacaacg ggtcttttaa gcttttcagg ccgtcaaccg agcaacagga gtccgtggag
    20401 ctctccgcag ccgcttccga aatggaatta cctgggcctg aacaggacca tcagtgcgat
    20461 gatgattaat gacggggtcc atcaggttcc cgcacataat acatcgatac gctttaatcc
    20521 acagttcccc cattgctccc ttaatgtcca gacaatcatc aaccaccatc agcccattac
    20581 atcgtacaca cgtcataact ctacctcctt tgaaagcctc ctgcttccca ctcttctcaa
    20641 cgggtgagtc aatgcatcac gcgtgccacg caacacgtga tatctcgaaa agcaccaact
    20701 aattaggtgt gtatgaatgc ggaaaatgaa aggagaacac tggacaatcg aatgaatttt
    20761 tggagtctac aatattttgt gataaaaaaa aagagcaaac acacattttg tgggtcatag
    20821 aaatattgac tataattcag gcatccggct cttcttgaaa aatatatttt ttgactttgc
    20881 tctccgcatt tatcgaccta tgtcagttca attaaaaata tggtgagatt tggcgggcta
    20941 gtggaatttt tgacagggtc ccctcatgag cactcatgag gttggttgta ggcctggtcc
    21001 attagaaaga cccttagttt tgaatatgag aacgtcaaag gtactcccca agccagcagg
    21061 cctagtgagc acatcaatct cttcttgatc agattcccta tcacctgctg agcctttata
    21121 atccagaatt ccaagattgc gcagccatct tccttgcggg ataagcccat ccacttcaaa
    21181 accgacctct tctgcaagga aacaagcagc ggtataatcc acatcagccg tcaaatcttg
    21241 ttggcccaca gcaaaaaact ggtcgtatat ttgtttctgg tgatagtaag aacgaagagt
    21301 cccgtttcta tgtcgatgtg aatataaatt tttcgcacaa tcgccataat cgataaaaat
    21361 cacataccct ggatccacaa ctttccatat gcttttaagg aatggtttca actccagaca
    21421 aatttctgcg acttgccctc gcccaagctg caccctctcg ccactcaacc gctctgctaa
    21481 agccatggtt gaaagaggcc tcggttgctc acaaaactct ccatcctcat ccaggtccac
    21541 atacaattcc aaaacttcct catgtcccat tcccatcaca cgatgaacgg gcaatgcatc
    21601 aagcacttca tttccgaata caaatgtcgg ttttgggggt aaagattcaa tattttggat
    21661 ggcctgcaat cctctttgct gttgattggc tcgcaaccct tggcttggtt ctagaactac
    21721 gtattcatga tcgttttcaa gacaagcaat tatatggctc cctaactgac ctgttcctcc
    21781 acctaattcc aagacccgta ggggaacatc taccgccttc tctgcatacg tgatggcttt
    21841 ggcaatggca tatccaaagg ccggaaatct tgcattggtg tcaaacggcc catcaggact
    21901 cccaattttg ggtgcctttg cataaaaccc catattttca tcatataagg ccgcagccat
    21961 aaagtccctg aaggttattg gcccttgcgt tcgaatcttg cccttcaagg cgttgccaag
    22021 ttccgtatca atccggttca aggcatgtcc aggctaattt gttgatggtc agaattaaaa
    22081 gaatcatgga atgaggacta gataggtcat aaaaattatg cgagggaaag tggccataca
    22141 aaaaaagggt gccattgctg gcaccctttt cctaagtatt caaaccgaaa aaaagtaaat
    22201 tacttaatta cagtcgattt agccccagct ggattcggag taattttgtt gatcgggcct
    22261 tcaacttcag gcaaacgacc ggcaccagcc gacttttgga tccgagcccg atttggatca
    22321 ttaacatcat tcatagcagc tttgctgcca aatccttgag aagacaacag actcgtctct
    22381 cctcctgcat tcgtttgacc aggatcattg gcggtagctt gacccgtcac tggagacttc
    22441 ccagcagccg gataccccgg gtgagctggt aacaaccctg gattggcaaa ggccattgat
    22501 agggcgaagg tgctagcacc caccgttcca agaaaaattc caatcccttt cataagaccg
    22561 ctcctttgtt ggttatgact ggttaacaat agaaaatggt ctggatatgt acatgcccta
    22621 atttattcag atagaaaagg cgatcttatt cagattggcc ggtagtgtca agtttactca
    22681 acaaaaatgg aaaaatttgc gaaatgccgt cgcttcaggg acttactttt tcaattaaac
    22741 cggacggcga ggccgatacg gagttctcgg tcgtggggct ggcatgagaa tagtgacggt
    22801 tgttccctta tctggctcac tagcgatact aatctgcccc tggtgctctt caacgacttt
    22861 tttcacggtc gccaaaccca acccggtccc tgatcgcttt gtggtgaaat agggttcaaa
    22921 caccttatcc acaaactcgg ctggaatggg cactccatca ttggcaataa ttatctgaag
    22981 ttccggcttt cgagcccggg tgagcatgac ttccacccgt aaatgacctc ccggagacat
    23041 ggcttcaatg gcatttttga aaatacacag gaatacatgt tgcattttca cttcatccca
    23101 ccggacttgg gccaaccgag ccggcacctt tttgataacc tcaatgcgat ttacacgaat
    23161 gtccgtcgac accaatgaaa aagccttatc caatagatcc tgaacccggc acatctttcg
    23221 gtcaagttcc aagggtttgg caaaatctag aatttcattc aacacggttt caatacgaat
    23281 catatcccgc atggcacttt ccacccgggt ttgcgaatcg aaatctaggt cggcgtcagt
    23341 cgtaatttct tctagttgag ccctgataaa cccgataggt tcacgaattt ctgtggccaa
    23401 aaacgaactc acttcatgca gtgcggcacg tcgctcttcc ttacgcacat cccctccctc
    23461 cggtggagca acaatcacct gccccttgag tacaggtttt tccggttgaa ccggggcgac
    23521 atccatgacg gaaaggtgac acgatgggtc ttcaaacaat tccaccaatt tggtcgtaat
    23581 ggcatcgaga cttttggaat cgctcccagt ttgatcaaaa ttattaggat tttttgacgc
    23641 taacgtgacg gtacccgcca ctcgtcctct gacgtaaaaa ggaacgacga ttgtcgacgc
    23701 gaagcgatca cgatacagct gcttatagtc atgaaaccgc ccttgggtcg aagccagatt
    23761 attgtcacag cgaggctttc gatggcgaac agaccacccg gacgccgaat catctagaga
    23821 gagaagctgc cccggagtca catcccgttt ctgggttccg aatacaccca acacttccag
    23881 cgtcgcggca agagggtcat aaatgctgac ccagccacga tcaaaagaca ggcccccctc
    23941 cacgtcttct gtcaaaatac tccctatccg ctcttgaacc tccgcagttg aataggttcg
    24001 agccaccgtg gcatgaccct cttccacagc caatgacaca ggttcattac ccacgaatgg
    24061 cttactcaaa atcagtgaag aaccaaatag ccccgctttc tttaattctg tagaaatatg
    24121 cgagacattg gttgagagag tcgtcttttc ccttttggtc agcgcggagt tttctctcct
    24181 ggccagcacc agcgtgccat atacccggcc cccaaacttt agcggtagga ctaacgcgac
    24241 cttgctccca ggagtgacca tgcgaactct gagtacgctc tccaactccc cgccaccccc
    24301 gttacggaca gcgacacttt tcaaatcctc cattgaaagc gcacgaagaa tcgcgcggac
    24361 ttcccttgga gaaaaccccc gggaaacctg ctcgactaat ggtccaccat catgagtcat
    24421 aatcgcaact aagaccgctt ccatcccgac ctcattaacc acagaggtca gaatcttttg
    24481 aagggtactt tgttgcttgg ctgctagtgt gggttcaaga cgattggaca tgcatgcgtt
    24541 tcctctcaga atatttgcgc acaatggcag tgtgggatat gccgatggtt aaggggaaaa
    24601 ggttctttcc taaaccctct atatattcct aaatctctca tcaacgccag aatggaactt
    24661 ccaagtggtt gtcccgttaa ttaatatggc gaatttttcg accggatgaa ggatgagtga
    24721 caatttcgat tcggccttgc tcggaaatcc agagatttaa tactccactg accaaactaa
    24781 taatgagcgc gccacccacc gctggccaaa atcctgagat aatgaatccc ttgaccagga
    24841 ttgagactag ataaaggaga aatccattga tgagaagcat gaacagcccc aacgtaacga
    24901 aaataaatgg agctgaaagc agataaagca cggggcggat gatggcatta agaaaggcga
    24961 ggataatcac tgccgcgatc cccgacgcaa cccccgtgat ttcaatgcct gggataatct
    25021 ggcttgccaa caaaaccgca aaccccgtga tcccgcaacg aataagaaaa cccttcatgg
    25081 ctgcatcata ccggccctgc ggtcgtggcc tcaagccgtg gccaccaata gtagggaaca
    25141 cctctttggg acaggggatt cattctgaag attccttgac tctgtaatcg aacaatcctc
    25201 gccacaaagt attaagaaga accgcagtca ggggtacaaa aataaagctt cccacgatac
    25261 ccatcagcat ttcaccaatc cagaaggtca tccccaagtt aaaggccagg acaccgtaat
    25321 aaagaccaat tcctagtatc aattcccatt tcactaccat tcggggaatg gggtctgtgg
    25381 catttgtgcc ccgctccatc aagcggtagc cgagaaggga gagaaatccg accacggccc
    25441 atcctgcaaa attcgcgagg ggaacaccaa aataaatccc ctcctggtgg taaccgtaaa
    25501 tatttcccag aaaccatcgt tcaccctgaa gggctactgg atcgatcacc acatccgaaa
    25561 aggtaaataa caccaccgtt aatcccacaa caggccatga gtttcttaat tccggatcga
    25621 atagccatcc ctgttggtcc accccttttg caggagttaa cacgaaaatg agagccaggc
    25681 agtaactgga atataataaa aatgagaatg agatggagtc catgaacggg atattagaaa
    25741 gatacaactc atggtcctgg gttgaacccg tataaaaata atcgccaaag gggatcccga
    25801 tccgggtcga ggcatattca caaataaaag caattgccca cgtgaggccg aagaatctgc
    25861 cggcccgccg ccatcctaaa agctgttgtc cgctatatag ggagacccct aaaaagacaa
    25921 agacataggg cctgagtatg aacgtgtttg tgagaagata aacgtattcc aaagcttaga
    25981 tttgtcgatg ggaaatggct aagacttgag aagggggatc aggcatatcc atgttggcga
    26041 aaccactcta ccgccttatt caaagccgtt tccacggacg tttggggaag agccaactcc
    26101 tggcgcgctt tcgtgcaatc ataatgcatg tgatatttgg ccatccgcac cccttccaaa
    26161 ggaattcttg gtgaacgatg ggtcaccaaa ttggcaatcc attgatttag atgcgctagg
    26221 ggaagcacca gcctccaagg caatcgaagg cgtggagcag gcactccggt tagtcgactg
    26281 agtatttgac aaacttcttt cagtgtaaga ttctgattgc ccagaatata ccgctccccc
    26341 actcgaccgc actccatcgc cctgatatgc ccgatggcca catcctcaac atccaccaaa
    26401 ttcatcccgg tttctatata cgccaacatt cgaccacgca taaaatccac aataatttgg
    26461 ccggttggag tgggcttgac gtcccgttcc cccactgggg ccgttggatt gacaataacc
    26521 acaggcaatc cctcttgagc catacggagg acctcttgct ctgccaaaaa cttagatctc
    26581 ttataatccc ctgccagttg ggccaacgtt ggaaagagcg tttcattccc aaggccccgt
    26641 tgatcgggca acccgatcgc accaatcgta ctcgtgtaga cgattcgttg aacacctgcc
    26701 tcacttgcgg cacgaaacaa atttttggtc ccttcgacat tgatggcata aaaaacagaa
    26761 gggttttgat cccacaacgc ataatgcgcc gccacgtgat agagcccttc gcagccggca
    26821 atggctttct tgagggacaa gtaatcctgc aaatccccat aaacggtttc gactggtaac
    26881 ccttcaagat ttttcagatt gctggtgggt cgaaccagga cacggacctc atcgcctttc
    26941 gcaacaactg cccgtgtcac cgcagcccca atgaagccgg aagaacctgt aatgagtact
    27001 ttcataaaat tcgggtgttg ccaaggagtt taatagcaac ccaacatgac ctacaccgac
    27061 aaaactgttg taaatgagaa gggttgggaa aaaacctaaa gtcgcttcaa gaccgtcgcg
    27121 ccattatata ggtggcaatg tctcgtaggg ggtcggcctg cttatcaaag gcatctaatc
    27181 gggttatcgc ccgcttgaca cattcctctc ccagttgcct ggcaccttca accccaaaaa
    27241 aagagggata ggtctgcttc cctcttcttt catcagtccc tgcgtccttt cccaattcct
    27301 cccgtgtccc caccatatcc aacacatcat cggcaatctg aaaagccagc ccaatatcct
    27361 ctgaatagtc cgttaggcac tgcatttgct gtgtcttggc ctcaccaata ataccgccga
    27421 tccgcactgc agcgcgaatc agacgtcccg ttttacaggt gtggatttgc tggaggtgag
    27481 gtaagtcaat ctcctggttt tcggcctgaa tatccattac ctgccccccc accattcccc
    27541 gatgcccaga accatgcgcc agttcccgca caatgtctaa ttgctgagca gcagagaaag
    27601 cctctgagcc atcaacttga ctgcataact caaacgccat ggtcaacaat gcatctcctg
    27661 ccaatatcgc cataccatca ccaaaaactt tgtggttggt taatcggcct cgccgatagt
    27721 catcgttatc catcgccggg agatcatcat gaataagaga atacgtatga acgaactcca
    27781 aggaagcggc gaacggcaac atggcatcgc ggtcatatcc cagggcttgg gcagtggcaa
    27841 tggtcaaaat cggacgaatg cgtttacctc cacccagaag gctatatcgc atggattcat
    27901 acagtcgttg aggatggacc gaaagcagag ggacgctccg ctccagaaac tggtcaaccc
    27961 gttggcgctg ctggtctaag taggcttgaa tgttcacaaa ggaacgaata gacgctgtgg
    28021 aaggatagaa aaaatagttc gtgaccctat caaatggatt tttggagtgt caaatgatgt
    28081 gtctataact tgggagcata agctcaggaa acggttccca aaaaacttct tcattgtttt
    28141 cacctgaatt actcagtata cttttgtgcc atgagcattc gaaaccgagc cggagattgg
    28201 gaaccccttt tgttgggtat tgaacccgaa atgtgcgggg tgtgtcacga aggcctatac
    28261 cggaacctta ccatgctccg tggaggatgg tcccgttgca gcatttgtga acatttcgtc
    28321 cattatagtt gtctggccag cgggaaggtc tcctatctga aaagacgccc gcgtgtgtgc
    28381 aaaacttgtg cagaccagca gactttcacc caacctgcca tgtccacgcc ttcggtgcct
    28441 gccgaagtca aataatggaa catccagggc tttcaacccg acctgctatg ggttctcctc
    28501 gactctcggc atcatttctc catttttttc tggctcccat cctctgcgca tttagtggct
    28561 ttcatactgc cagctatctc ttcgtcggtc tctacacgtg gcctcgaacc agtcgggtat
    28621 ttgtcctgac cattggtatg cttatttttt cctatgaatt catttataag aaccagtcct
    28681 cacctcctac ttccacatgg aaatattctt cctttgaatc ggtcttctat acctgtgctg
    28741 ttccctatat aataggagca ggaacactct tggtccttgc gggcctgtga gaatagggca
    28801 gcttttctgc caataccctt gaacctgtat tgatctgcac ttctagagag gatccaacca
    28861 tgaaaaatgt ggaattgact gtcaatggaa ctatgctaac tattacggta gacctgtcga
    28921 aggattttgg tccttcttca tctgggaaaa cgacgatcat tgcctcaacc gaaggcaatg
    28981 tttcgattcc cgatcgagaa gaaaaaatcg ggctgaatat ttacaagaaa aaatcctaga
    29041 ctttttcagc aagctcggct tttagaatct tctagtaaca cctgccctgt ccacgattcc
    29101 ttgactttca tcacggcttg cggagtaccc tccgccacca cacatcctcc ttgatctcca
    29161 ccttccggcc ccaaatcaat gacccaatcc gcgttctgaa tcacatccac attatgctca
    29221 atcacgagga ccgtattccc cgtctccact aaccggtcaa gcacatccaa caaccgttga
    29281 atatctacaa aatgtagtcc cgtcgtcggt tcatccagaa tatagagcgt acgaccagtg
    29341 ggtcgtttcg agagctcctt ggaaagcttg acccgctggg cttctccccc ggacaaagtg
    29401 gtcgccgatt gcccgagttt aatgtaatgc agacccacat ctgataaagt ttgcagtcga
    29461 atccgcagtg gcggaatgtt cacgaaaaac tccagggctt cagccacggt catgtttaag
    29521 acatctgcga tcgttcgccc cctgtaggtc acatccaagg tttcgcgatt gtaccgttgg
    29581 ccattacagg cctcacaggt tacataaata tctgggagaa aatgcatttc aatttttatg
    29641 aggccatcac cctgacaagc ctcgcatcgc ccacccttga cattgaaact gtagcggcct
    29701 ggcttgtagc ctcgcactcg agactccggt aattgcgcga acagatcacg aataaaacta
    29761 aagaggccgg tataggtggc gggattcgaa cgcggggtcc gtccaattgg ggactgatca
    29821 atatcaataa gtttatccag ctgatcaagc ccacgtattt cccggcatcc ctcaaacacc
    29881 ggcttcttct gggaaaaacc ctgtcccagc gaccggaaca acacatctac gaccaaagtg
    29941 ctttttccgg atcctgacac accggtgaca caggtcaaga ggcccaatgg aaaatttacg
    30001 gtcagatccc gaagattgtg cttttccgct cccacgacgg tcaaaaatcc tttggggtgc
    30061 cgtgcacgct gaggcaagga cacccggcga ttcccattta gatattggcc ggtcagcgag
    30121 gtggggtgag ccattaccac cgcgggagat ccatgggcga ctaattctcc accctccacg
    30181 ccagctcctg gccctaaatc caatacataa tcggcagcgc gcatggtctc ggcgtcgtgc
    30241 tccaccacca cgacagtatt ccccatatca cgcaatcgta acaacgtttt gagtaaccgt
    30301 cggtgatccc gctgatgcaa cccgatactg ggttcatcca gaatatacaa cacgcctacc
    30361 aatcccgatc cgatttgcgt ggccaatcga atccgttgcc cttcccctcc ggacaaagtg
    30421 gcagagggac gatctagagt gaggtagccc aatccgacgt tgagtaaaaa ctccagcctc
    30481 tcccgaatct ctttcagcac tcgctcccca atcatctgtt cgcgatcagt gaggttcaac
    30541 gcattcataa atgccaaggt attccgcaca gacagatgcg tcagttcagc aatagactgc
    30601 cctccaattt taatggcaag gctttccttc ttcagtcggc ctccgccaca cttctcacat
    30661 ggggtatcga aggtccattc ctcccattcc gaatgagttg aggcttcata cccaatccca
    30721 tcgcatccat cacaagcacc atgggggctg ttaaatgaaa aaattcttgg ggaaatttct
    30781 gaatagctga caccacactg aatacatgcc aggtgttcgc tatagacacg gacttgatca
    30841 tcttctgtca gcaccccaac gaggccgtgc gccaacttga gggccgtttc taccgaatct
    30901 gccaaacgac gcgacacccc gggctctgac ttgacgatga gtcgatcaat cacaatctct
    30961 attgtatgtt tacgttgttt atgtaaatga atagactccc cgagatcccg aatggctcca
    31021 tcaatgcgag cccgaacaaa tccggccttg cgagctgcca ataattcttt gcgatattcg
    31081 cctttgcgtc ctcgcacaat gggtgccaga atatggattt ttgtgcctag aggaagcacg
    31141 tgaatggcat ccaccatttg ttggaccgtt tgcgctgcaa tggcattccc acagaggtag
    31201 caaaagggct ggccgattct ggcaaacaac agacgaaaat aatcatagat ctctgtcacc
    31261 gtccccaccg tggaacgggg attgtgactt gtcgtctttt gttcgatgga aatggcaggt
    31321 gaaagccctt caatggaatc cacatcaggt ttcgtcattt ggtcaaggaa ttgcctggca
    31381 taggcggaga gtgattcgac gtatcgccgc tggccttcag cataaatcgt gtcaaatgcc
    31441 aaagacgact tacctgatcc acttaggccc gtaattacca ccaactgatc ccgaggaaac
    31501 tccaggtcga gattcttgag gttatgttga cgagcgcccc taatcacgat tgagttgttc
    31561 atgggaggcg agtatatcat acgaatcccc ttgactgtgg cttctctttg aacaatggaa
    31621 ttgaccctta cacaaaaggt cggatacaat ctggcaggct tcatgtatca ttttgaatag
    31681 acggaatcgt attcttttcc ccaaccttac tttcatttcc cttgatgagg aatttcatga
    31741 agaatctgaa aacagcatgt atcttgactg ccactattat tctgacgagt cttttttcgc
    31801 ttgctccacc agcatgggcc gccaaatacg aactcacccg aaacagcatt gaggatgcca
    31861 aggatgtgga tgccggagac atttccctat tccgcgtaaa actcggagat gctgaagcta
    31921 tcgccgttga agccctcgtg gaagcaagga tttctggcgt tcgggcagag caagaaggta
    31981 cctttattct tctttgggac aaggaaaatc caactggtgc aatggcagga gtccgtatca
    32041 cggacgggaa agtagattta atttttataa acaatcggtt tgcccataaa gtccgtggga
    32101 ttttccgaca catcctcggg gccaaatcca caaaagaaat ccgtgacctg ctgggaccag
    32161 aggatttccc caatgaaaca ctaatgggcg ccaaactctt gtatgaaaag caagggtttc
    32221 tcgtgaattt ccttgaacgg caagtaaatg ttgaattctg tcttggctgt caggatctat
    32281 tttcaggatt ctaggaaccc tgttgggggc tgcagtttca tctcacttcc taagtctcaa
    32341 tctgctttca atttttaagt catttcccaa aggaggaatt cgtcctccca cttctgtttt
    32401 cccaacgtcg tcaagaatgc cgttctttta atttttctgt ttagactgat tccctttatg
    32461 gacgaccgaa ttcttaccac ccatctcata gaagaagagc aaggccttga ggggactctt
    32521 aggcctcaac gcttaacgga atatatcggg caggagcgga tgaaagaatc gcttcgggtc
    32581 tgtattgacg cggcgacggg aagaggagaa gccttagacc atgccatttt ctatgggcct
    32641 ccgggattgg gtaaaaccac catcgctcac atcatcgcca aagaaatggg aggcacgatt
    32701 cgttctacct ccgggctggt gctcacccat gcgggagacc tagccgccat tttggctaat
    32761 cttcaaccac gggatgttct gtttatcgat gaaattcatc gattaccgcc agcagccgaa
    32821 gaggtcttat atcccgccat ggaggactac caaatcgatt tggtgattgg acagggcccc
    32881 tcactgcgca ccatgaaatt agatctcccg ccttttacac tcattggagc caccacacga
    32941 gcgggctcac tcacctctcc tctgcgagaa cgctttggac tagtttatcg attggattat
    33001 tatttacctg aagagctgca ggtcatcatt gtgcgttccg ctagactgct cggcgtccac
    33061 attgatgatg agggggcagg ggaaatttcc tgccgggctc gtgggactcc ccgtattgct
    33121 aatcggttaa tcaagcgtgt gcgtgacttc gcggaagtca aagcccaagg acgtatcacg
    33181 agatcggtcg cacaggaagc tttacaatgg ctaggcgtcg accatgcggg ctttgatgaa
    33241 atggaccgca aaatactatt gaccataatt aaaaaattta aagggggacc ggttggcatc
    33301 gacgccttgg ctgcagcgat ccaagaagaa aaatccacca tagaagatgt gtacgaacct
    33361 tttctacttc aatccggata tctggatcga acggcccgag gccgacaaat cacctcaaaa
    33421 gctttgcaac attttggaga aaatcctaat ctcttttcga tgggcatctg accattcttc
    33481 cctcctacct ttaatctata caactttcac tttcactttc cctctcgcac gtttataaaa
    33541 aaataaccga cccctccctc cccttccttt gctgtattga cggtatccga aaaatgatcg
    33601 gtatagtagg gaaaactgac aaacaggcca tgagtcagat gaaatgaacc tgttctgtct
    33661 tatccattta tacgaaagtg aggggatttc ctaatgaata tacgcgcctt atcatcaatt
    33721 tatttttttt acagaaagac ctccctatca ttgaagtatt tatccctgag gaatgtcgtt
    33781 tttctggtcc ttttctgttc cctgagcctg gtaaactcag tctcggcaaa tgaactacat
    33841 atcgagtttg ggaattcctg ggtggaagat ggctacattt tccatgtgga cattcaatta
    33901 ccgccctaca atgtttattg tacaacatct gggagagggt acattggctt taacgtgtat
    33961 tatgaagaca cgagaagcct ggaaggagag tgggcctttg gtatcgctcc gtggcctgag
    34021 gggcctgcat ctgattccaa agtggggtca catatgactg ctgtggctcc tcaagtatat
    34081 tgcaccagtt tttccccttg tacaattgag aatattcaaa tcgtaaaggc ttggtgccca
    34141 ccactagaag gagaaagttt ttatgcacaa taaatcttca ccatcctttg tcttcccaga
    34201 aacacccatc tattttcatg ctacataaaa acatttagta aatacattat aagaaaacct
    34261 gtctattcca cagcaggagt tactctatac attaagggaa atttaaaaaa ttctcagtat
    34321 tagggaaggc taccttgcag gcccaacagg ccgagttccg gaatccagga tggcttccac
    34381 atcttgcatt cccgttttcg cacgatcgac atataattgg taggccaagc cttggtaggt
    34441 ttcaataatc cgtttgtagg tacccagggc tttgttgtac tgttgggatt ccgaaaagta
    34501 acgggccaag gctaaccaat tctccgcagc tgtctctttt gctgttttga taattttcca
    34561 ttcctgcgtt ctctcaccct gcctcatctg gcccacacga cggagcaagc gctcttcact
    34621 tcttagcgcc agtacctcga tctgttcatt atctgccaaa gcccgggcag gctgatggga
    34681 ttgcaaatgg ccatagaaca tttccacatg gcctttaatg acatccgcct gttgctgata
    34741 cctgtctcct gtgcatgcgg aaagggtact taccatgagt agggcacaga acattttcaa
    34801 ccagacacgc ctcttcatgc cagaaccaga attggaaata tcgttgttca tgttttcctc
    34861 gcaaatacaa caatggtcac cttattttcc cccctccaac aggcatcctc gataggatct
    34921 cgtccgtaaa aatcagcaag ttttactatt tggcgaagac cacaagactc tccacgtctt
    34981 acccagttca aactttgcct gatggattgc cgagattatt gacgactaag gctatgattc
    35041 gatcagtcaa cgcttctttt actcctcata aaatctgcaa ctctcactcc tgactttaaa
    35101 aaatcaaccg gggcgcaatg gctgatgcct gaaatgtact caggtgatcg ccataataca
    35161 gcccaaatac aatgacctca tcatcaggat ttaataaagg tcatgcagga acatgtaagc
    35221 ggactattaa caacccaatt aacaataatg ttcgtttata aatacttgat ttacgggatt
    35281 atcgcgagcc ttgatgccga cgtgcggacg actttcgcca tattgccaag ccatcaaaat
    35341 gcctggggta ttgattccta tacgactgat gccttggcct ctcccatcgc aatttctgtc
    35401 atgctcgtat ctcacgcctg cagttcacta cattgcttcc ctccacaagg atgagctgtg
    35461 gtcattgccc ggatctctga ttgccctaac cctgctaaca ggggaattct gaatcttatc
    35521 atcccccaaa ctttatcatc ctattgcatc ccccgctatt gtcggcttgc tcccaaagtg
    35581 cccttcacgt agactgactt cctttctgtc ctcagaaacg acaagaaaca tgctccacat
    35641 attcttcctg ccggtcacag cagttcgact ctcccattat cgttttttga
//
LOCUS       DAORAQ010000014        35545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_257_length_35545_cov_61.440462, whole genome shotgun sequence.
ACCESSION   DAORAQ010000014 DAORAQ010000000
VERSION     DAORAQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 35545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35545
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_35545_cov_61.440462"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            786..1079
                     /locus_tag="PKM72_02635"
     CDS             786..1079
                     /locus_tag="PKM72_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNP59706.1"
                     /translation="MNHTNLLSLLTNGREEIVRRFGIKKLGIFGSAARDEMHEGSDID
                     VLVEFQSSPTFDAYMDLKFYLEDLFHSSVDLVIEDTVKPRMRPLIEKDLIRVA"
     gene            1333..1461
                     /locus_tag="PKM72_02640"
     CDS             1333..1461
                     /locus_tag="PKM72_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59707.1"
                     /translation="MDGSCLIDAGFRPGSRTTFVSAKVVKTIDAPFGLIKEEGRQL"
     gene            complement(1458..1634)
                     /locus_tag="PKM72_02645"
     CDS             complement(1458..1634)
                     /locus_tag="PKM72_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59708.1"
                     /translation="MGQVIVRNLDDSIFRSSPRAYARIDRQIVDPTPAGWPNGGTLLS
                     ASGPCLSGASWAAL"
     gene            complement(1901..4054)
                     /locus_tag="PKM72_02650"
     CDS             complement(1901..4054)
                     /locus_tag="PKM72_02650"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNP59709.1"
                     /translation="MNSALHDKTIAKELKITDKQVTATVSLLDDGATVPFLSRYRKEV
                     TGGLDEVVITSIRDRVAQLRELDKRRDVILASLEEQGKLTDVLQEQVQAATTMTELED
                     IYLPYRPKRRTRAMIAKERGLEPLALSLWAQDAQLNVEAEAQKFLNPELSVETVEDAL
                     AGARDIMAEWISEDLQARASMRALYLEQGTFKTTAVRGKDVQGSKYRDYVEWEEPVAK
                     APSHRVLAMRRGEVEGFLTFRVLVPEAEALSILHRLFVKGKGPASEQVILAVKDSFTR
                     LLALSMETEARLVTKTRADQTAIEVFAQNVQQLLMAPPLGQKTVLAIDPGFRTGCKMV
                     CLDRQGTLRHTETIFPHLGAAGAAKAGDTVVELCQRFQVEAIAVGNGTAGRETETFLR
                     ALKFPSAIPIVMVNESGASVYSASPVAREEFPDHDVTVRGAVSIGRRLMDPLAELVKI
                     DPKAIGVGQYQHDVDQSILKQRLQDVVISCVNRVGVDVNMASPQLLTAVSGVGPQLAT
                     NIVAYRQEHGPFASRTALKKVPRLGAKAFEQAAGFLRITDGEHPLDASAVHPERYAVV
                     NAMAKDLGCTVKDLMKDPARQRTIDLTRYITEEVGKPTLTDILTELAKPGRDPRQQFE
                     AVKFDEGVQSIEQVKPGMIMSGVVTNVTAFGAFVDIGVHQDGLVHISQLANKFVSDPN
                     TVVQVNQQVKVTVLEVDIPRKRISLSMKAASNGKP"
     gene            complement(4151..4441)
                     /locus_tag="PKM72_02655"
     CDS             complement(4151..4441)
                     /locus_tag="PKM72_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59710.1"
                     /translation="MMRAWLVVCVGSLFLYGCTDVKYVKAGATEADLEADRVDCRSQI
                     LMPSPGPGIPSGQMGKPGVSQGFITRSAEQSAQQEVDQCLRAKGWELEPQPK"
     gene            4561..5268
                     /locus_tag="PKM72_02660"
     CDS             4561..5268
                     /locus_tag="PKM72_02660"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNP59711.1"
                     /translation="MNTFSKIQRLSFLFCVILSLPGCAQKTQSLDFSGFLTDYNSLRP
                     SPDDSGAWTYRKPGVNFKDYNTIMLDPLVIFPSQHSEYGGLDGLTAWRLALAFQESMS
                     RALAGGYVIVKDPGPGVLRLRAALTDVMLERPALESSIPLLPLANDFLIQGAEKISGM
                     NALEGEAAIEAEILDTQSQERLVAYVEKRMSSEVLLTREKDSLGPVLEIFDYWARKLR
                     QRLDEERGIREYQKDIQ"
     gene            complement(5481..7838)
                     /locus_tag="PKM72_02665"
     CDS             complement(5481..7838)
                     /locus_tag="PKM72_02665"
                     /inference="COORDINATES: protein motif:HMM:NF018620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HNP59712.1"
                     /translation="MTDRPYVRIVFILLLTIIVGGCALYAGMQLEQQFGPSSPQNRLT
                     KTTGVTTVSYRQDVKPILEARCIVCHGCYDAPCQLNLSAHEGIERGANKELIYNPGRL
                     LAMEPTRLFVDAHSPSEWREKQFFPVLNERVQTKEANLQASLMAKMLLLKRQHPLPAV
                     SPLPPSFDFGIERPQYCPTLPEFDAFARDNPLWGMPFGLPGLGDEEHATLMKWLEEGA
                     DDEAPAPVTAGVLSNISEWETFLNGQTPKEQLMSRYLYEHLFLAHLYFDDLAPKQFFR
                     LVRSTTPPGEGIDLIATRRPYDDPKVDRFFYRFEPVRTTILAKTHMPYALNPDRMSRY
                     RTLFLEPAYGVPTLPSYDPETSANPFETFKLIPVKSRYQFLLDEAQFTLMNFIKGPVC
                     RGQVALNVINERFWIVFSNPNSTTIAQGAQFLEKEAKNLQMPIVEQSNATPLGTWLKY
                     SQLEKKYVAGKIKFIQQHVSTPEAISLDLLWDGDGQNDNAALTVFRHLDNATVVKGFV
                     GEEPKTMVLLGYPLLERIHYLLVAGYDVYGNIGHQLNSRLYMDFLRMEGELDFLTLLP
                     ERTRTQEWKYWYRDAGDKVEDFGAVYFNSINRQTGIQYRTADHKLELIGMVRERLAPV
                     LHRPYAIENSGNDHVRQQLEKLSHLQGTAVSWLPQNAILSIGGSHAQVVTLIHDNGMS
                     NVSHLLFEQERRLPEEDTLTVVRGFLGAYPNAFYQVDESTLEEFVSAVEHLGSEEDYQ
                     ALLNRFGVRRSDPDFWKHSDAIQAAYKNESPVEAGLLDYSRLENR"
     gene            complement(8379..9692)
                     /locus_tag="PKM72_02670"
     CDS             complement(8379..9692)
                     /locus_tag="PKM72_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HNP59713.1"
                     /translation="MKEVVAVRARLHILGLLLMGALILPPSIQAGGLFLTEIGTPDVG
                     LAGAGWAARAQDASTLFKNPAGMSLLEGAQFQGGAQLLYGDIGFTSNGKSTPSGGGGG
                     NPIDVFPGASGFYVHPLGDDWRVGFGIFSNFGLGLKYKGEWVGRYYVKDALLAGVSFM
                     PTASYRVNEYLSVGGGVNVMLGAVKQTVGINNLGSTNDGQMQIKDQAVGVGGNIGVLF
                     TPTRGTRFGLTYQSPIDLNFSDKPKFSNLGPIGTALQNNGLLNKKLDVGMTVPQSLMF
                     SAYHDLTGAWAIMGDFGWQDWSEFGKVDIGVDTANPTNLVANLNYQDTWHVAFGTQYR
                     VNPAWVVSTGFAYDSSMVKDKNRTLSIPVGETYKFGFGALWQATRTLNLGFSYELTWI
                     GDMPVDQNRGPLSGRVSGDFKNTTLNFFALTVTWGEGVKMGPGGA"
     gene            complement(9766..10095)
                     /locus_tag="PKM72_02675"
     CDS             complement(9766..10095)
                     /locus_tag="PKM72_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59714.1"
                     /translation="MKVVPWWSSCVILALASCGGGNLQHMDATPYAQMIERPNGECPH
                     SKIEQGIVNKHPDATIMATVSEITGDNVVTTAQIPVKAKETVYLGCAVLPSGQDADRK
                     ILDVQFE"
     gene            complement(10160..10855)
                     /locus_tag="PKM72_02680"
     CDS             complement(10160..10855)
                     /locus_tag="PKM72_02680"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNP59715.1"
                     /translation="MIRLPLHVVLILLVGVSGCASTQQTRSVEQSGFLGDYSMLKEGK
                     KDETLLIYKNPQANWRKYQKIILDPVTLWIGKDSQLKDVSAEDRQRMADLFWAKLHEA
                     LKTDYEIVTTPGPDVMRLQAAITEAETSNPVLDTVSSIVPQLRVLTGVKGFATGVSGF
                     TGSASVELKVTDSSDKTLLAAAVDRRGGTKSLSGVTNSWNDVEEAYRYWAEKVRWRAC
                     LLRGGENCVEPEA"
     gene            complement(11092..12960)
                     /locus_tag="PKM72_02685"
     CDS             complement(11092..12960)
                     /locus_tag="PKM72_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59716.1"
                     /translation="MTRTQSHGLWFFIIFTAVMMTGCQSPVGVTRVDSETVRHQLTKN
                     VISSGETSPYTENVLRVTDLTEAYFDDPREALNTLHHIFLAEEQRKGPVAFALAELSF
                     LYAHKTNQPTYYLAAALYAYLYLFPEDSSNHFNPFDPRVRIAADLYNRSLTTGFISKE
                     RSVMILQSGSYTLPFGTLQVALPENRLQWENRTLTNFIPVAELEVRGLHNRYRQAGIG
                     VPLAADQIPLGQEEGLQIAKGKMPVTAFMRLPNLMEQLKKGTIQGDWEVYSAYDQRTV
                     RIQESDVPLEIEFTSSLAASLADDRVWARELKGFFMGDSLAGESGELIALEPYRPGRI
                     PVVFVHGTASGSGRWADMTNDLMADPSVRDRFQFWFFTYDTGNPILYSAALLRETLQT
                     TVSDLERKYQDPALQAMVVLGHSQGGLLSRLTAVETGNIFWDEVSNQPLDDIKVSDQT
                     RDLLERMLFITPLPTVKRLIFIATPHHGSYVAGSWGAHQLAKFVKLPGRLMSGVTDLL
                     SLNLDTLKLDLQGMNVGSVSAMEPGSPLAKGLAQTPLAPGVSGHSIIAVEGDGPMEDG
                     NDGVVAYQSAHLEGMESEFIVRSGHSCQSNTHTIQEVRRILLLHALETCKTHGLCA"
     gene            complement(13180..13653)
                     /locus_tag="PKM72_02690"
     CDS             complement(13180..13653)
                     /locus_tag="PKM72_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59717.1"
                     /translation="MKQFIMCTVAAVILCVTPVFAENAQITNMQILIDKVKADKKLLI
                     ASNMDLTDKESKDFWPLYEGYQKELEHLNQHLGGLINEYAEAYNKGSVPDKVAKDLIN
                     ESIKTEGAEVKMRREYAEKLSAVLPAHKVARYLQMENKIRAAIKFDLAANIPLMD"
     gene            complement(13877..14776)
                     /locus_tag="PKM72_02695"
     CDS             complement(13877..14776)
                     /locus_tag="PKM72_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNP59718.1"
                     /translation="MLRTLLAACFAVAGLVALVGFCTGRFRWMAAGSYLSLFILLFVW
                     WSMIEPSNDRVWQAEVAMLPYATFDGNLITVHNIRNFDYRTETDFTPAYYDQTYDLTK
                     LDSVDLVAAYWMGPMIAHIFLTFGFGDDHLAISIEARKEATEGYSSIKGFFKQYELIY
                     IVGDERDLIRVRTNYRKDPPEDVYLYSVTGSPENLKRIFLGYMKTINELRERPKFYNT
                     LTANCTNVIWMHTRLNPGHVPFSWKILLSGYTPAYLHEQGKLGAGPSFEALQARAHIN
                     ERAGQADQAPDFSSRIRAHLLSP"
     gene            15564..16058
                     /locus_tag="PKM72_02700"
     CDS             15564..16058
                     /locus_tag="PKM72_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNP59719.1"
                     /translation="MPIVFSRRLRISGLILLVIGFMSACSTLPGNFETPKISIAGITP
                     KDVTLFEQRFEVQLRIQNPNNFDLGLNGIRFDIELNDKEFGNGMSPAKVIVPRLGSEV
                     VTGEVITGLDSFLRQVQGMNSTAAKLRYHLQGTAFAESPGNFTIPFDDSGEVDLHFAP
                     AAQE"
     gene            complement(16075..16548)
                     /locus_tag="PKM72_02705"
     CDS             complement(16075..16548)
                     /locus_tag="PKM72_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59720.1"
                     /translation="MRTLISKRVVLATIMSLAGFLQGGLDRASVQAAVKGQEVLMAEV
                     HPPREIGGSYPNLSNNDGEGAEEKVRGERLFQRNLQSGVLQQSGNGARLQLKSPGKLP
                     GIELGVEMHSLQAFQSVIPSENLPQGLDVKQKQPILLRPSINAPDYNGGFLRFTW"
     gene            complement(16730..17068)
                     /locus_tag="PKM72_02710"
     CDS             complement(16730..17068)
                     /locus_tag="PKM72_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNP59721.1"
                     /translation="MDKVMWKVSGLVFVTSSLVGLIGGLLAAPQSGLRTRKKIHRGYE
                     HMKKDIKGQVKQEVHDLKGAVANVARQGNAYREKLLAWKGFSEWQQGFLDFPRAAPVK
                     VLRWHPKDVQ"
     gene            complement(17246..17977)
                     /locus_tag="PKM72_02715"
     CDS             complement(17246..17977)
                     /locus_tag="PKM72_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNP59722.1"
                     /translation="MAVSERKKREYAQRESLIIDTARKLLLDRGYLELNMDQIAEVTE
                     YSKGTIYQHFSCKEEILVAMLSESADKAGQFLLRGSSLKGNPRERMAAMALGYDLFVC
                     LNPDHFKAKLLVQNESIMRKASPTFQKKMDASNQENMRLVAGVLRDAVAEGHVQLKEG
                     ITPEEVAFGLWTGALGAYVLMSSEVNLHALGITDPRKAVWENMHALLDGFGWRPLSSE
                     LDYEQTRIRILKEVFPQEAAQVGKA"
     gene            complement(18354..19670)
                     /locus_tag="PKM72_02720"
     CDS             complement(18354..19670)
                     /locus_tag="PKM72_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNP59723.1"
                     /translation="MTVPPATAQEPFARARIHPEGLVTVGQPVELVVEVFVSTWFAGA
                     PQFPSLNIDNALVIPPGRSSNLNEEVNGEKFFGITRSYTLYPQVPGRYQVPSIEVRIK
                     PGLAKTTTVVRTPPLTLEAGIPREARGLPYFIGAKELTVSQIVQPTNRLFKVGDVITR
                     TVTTTVYDSLSLVLPSVKFLALPGLAVYPSPPVVTDEGGERGERIKGQRTDAATYVMQ
                     EEGDYELPAVEVVWWDVANLRIQRSTAPSISFHVDPDPAYGAEFALSDKPDSGLEKDM
                     SGGNGREWVPWVLTIIPLMVAVILLGLNFRYGSHLTAWWQEKQRRHRQSESVHFSAFR
                     RACRKHDPREAMRCLLTWLEHVCPFDRTVTVSWLVTETSAPDLAAAVSELEARLYGPS
                     PPAESWNGDALNQSVSRQRKRLLSSSPIQQTQTMLQPLNPSTGVSG"
     gene            complement(19756..21357)
                     /locus_tag="PKM72_02725"
     CDS             complement(19756..21357)
                     /locus_tag="PKM72_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNP59724.1"
                     /translation="MLDQVTELHFLRPLWLLGLGAALFLYLLIRLRTSVDRQWHGIIE
                     PHLLKHLKIHGDGGSRFQPLHLITLVVALGSLGMAGPAWEREQTPFTEDLAPLVIVLD
                     LSSTMNAIDVPPTRLERVKQKIRDLLAQRKNARTGLLVYAGSAHSVLPLTNDPSILDL
                     YVSDLRTDVMPVQGKNPALALQVAEGMLQRDTVAGTILFVTDGIALEHLPVFRTHQES
                     TRDQVMVLAVGTREGAPVRIGDNRFLNDANGRRLIARLDYEGLEALSKQAAIEVISTT
                     IDDKDVERIQDSVQSHLQAVLAKDEQVRWKDFGYFFTIPVGLLALFWFRKGWTVPWIP
                     MFLLFLLPGCSANDGEFSFIDLWLTADQQGRFYLERQDYATAADRFDNPMWKGITYYQ
                     AGEYESAIDCFARLSTPEGYFNLGNAYAKIGDYPQAVQSYDMALKLRPDWSEALENKA
                     LVAGLIPASPEEPPGSDDPGGPTFDPDAIEFSEKGKEGQSGEVEMEQLSDEQIAEMWL
                     RRLHTTPADFLRTKFALQAEQPGER"
     gene            complement(21350..22345)
                     /locus_tag="PKM72_02730"
     CDS             complement(21350..22345)
                     /locus_tag="PKM72_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNP59725.1"
                     /translation="MLEFEYLWIFFLLPAPLLIWLFFPPYHEPQESVRIPRFELLTGA
                     IGGRPTRGAVVLRRNWLQGFLAPVFWILIIIALARPVWIEEPIERIESGRDLLLAVDL
                     SGSMETRDMRDLDGRQIERLEAVKGVLDNFIARRPGDRIGLLFFGTAPFMQVGFTLDH
                     EVVRTLLSEAEVGMPGPQTMMGDAIGLAINVFENSEAEERVLVLLTDGVDTNSKVPPE
                     KAADLAKTKDITIHTIGFGDPSLAGEDLFDEAVLQTIAEKTKGLSFMAENREALEAVY
                     RELDKLEPQNFETLSYRPTHPLYFWSFGAAVILLLFYHASLTVWLALRSRGGRDA"
     gene            complement(22338..22850)
                     /locus_tag="PKM72_02735"
     CDS             complement(22338..22850)
                     /locus_tag="PKM72_02735"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HNP59726.1"
                     /translation="MDEIHGRQDFPSLPGLHDIVMPEPVAWVPQTPGWYLVAFLLSVG
                     LVWTAVCFRRRWMLSRYRKSALIELDRIEALALHPESQVKGLAELPVLVKRTALSFKS
                     REEVAALTGEAWLRFLDTSYGGTGFAQGVGRLLIHAGYEVPTSSPHEKQEKIVDLFRL
                     VRYWIRRHHA"
     gene            complement(22872..23813)
                     /locus_tag="PKM72_02740"
     CDS             complement(22872..23813)
                     /locus_tag="PKM72_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNP59727.1"
                     /translation="MIDTGVYVELNELVALQYKARGVSFLPRQPVHSILSGRHASRMR
                     GRGLDFEEIRGYLPGDDPRTIDWKVTARTRKPHVRVYTEERDRPAIVVVDQRLEMFFG
                     SKRAMKSVVAAQVAALASWRVLSMGDRVGGILFNDSDLVEIRPHRSRRNVLHILRTLV
                     EMNHSLQADSDVGSNPSMLNRVLKNLLRLVKHDCLIIIISDFDGIDGETRPLITQLSG
                     HNDLIVVPVFDRLSRELPDRGRLVVSDGTLQVEVDTAKGSIRKKLLETCERRMKTLME
                     WWEELAIPVAPLLTDRDPVDQIRELLGQAQARRSKRR"
     gene            complement(24020..25009)
                     /locus_tag="PKM72_02745"
     CDS             complement(24020..25009)
                     /locus_tag="PKM72_02745"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNP59728.1"
                     /translation="MIARDSILELKSRMSRSILGQEHVVERLLIGLLANGNLLVEGLP
                     GLAKTRAVKSLARQLNADFSRIQFTPDLLPSDITGTEVYHNVDGRGEFHFEPGPLFAN
                     IVLADEINRAPAKVQAALLEAMEERQVTVAKKTHKLPDLFMVMATQNPIEQEGTYPLP
                     EAQMDRFLMHVYIDYPDEASEIEVIRLVRREERPQPAIHEAKASSAEAAPISQEVVFA
                     ARDEIHKVYVSENIEKYIIDLISATRHSDRYGEEMGKLIQIGASPRGGLGLDKCSRVH
                     AWLNGRDHVTPDNVRAVVNDVLRHRIMLSYEANASGVTADQVIEKLIKVVAVA"
     gene            complement(25104..26675)
                     /locus_tag="PKM72_02750"
     CDS             complement(25104..26675)
                     /locus_tag="PKM72_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNP59729.1"
                     /translation="MVQKRLANISGHSLLALAGLVLCAGLSPAFAQDKPNILVIWGDD
                     IGQSNISAYTKGMMGYRTPNIDRIADEGMIFTDYYGEQSCTAGRSSFITGQSVYRTGL
                     SKVGLPGAELGLKGEDPTIAEVLKPLGYATGQFGKNHLGDKDEHLPSNHGFDEFFGNL
                     YHLNAEEEPELPDYPNAKDYPNFQKRYGPRGVIHSYADGKIEDTGPLTKKRMETVDDE
                     TSAAALDFIDRQHQAKKPFFVWWNGTRMHFRTHVKPELRGISGQDEYSDGMVEHDRHI
                     GLFLKKLDDLGITDNTIVFYSTDNGPHMNSWPDAAMTPFRGEKNTNWEGGWRVPAMVR
                     WPGKIQPGSVSNEIMHHMDWLPTFAAIAGDSDIKEKLLKGHQAGNKTFKVHLDGYNFL
                     PYLTGKEKKGSRDEIFYFTDDGDLSALRYRDWKLVFLEQRAEGTLKLWSNPFTSLRIP
                     LMFNLRRDPYERAQLTSNTYYDWLLDHVFLLVPAQEYVGKFLATFKEFPPRQKAASFS
                     LDQVMEKLKEGGGSQ"
     gene            complement(26793..26987)
                     /locus_tag="PKM72_02755"
     CDS             complement(26793..26987)
                     /locus_tag="PKM72_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59730.1"
                     /translation="MPEDVAKNLIGEALKTEGADVNMRHEYAEKFTHVIPVINVARYS
                     SNGEQKPDCDSRANLRRIFT"
     gene            27237..27383
                     /locus_tag="PKM72_02760"
     CDS             27237..27383
                     /locus_tag="PKM72_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59731.1"
                     /translation="MGTILAGQLMTMRMSLLLVGLMLGKNVFAKPNVAAVGQFKEMNR
                     SDLI"
     gene            complement(27403..27714)
                     /locus_tag="PKM72_02765"
     CDS             complement(27403..27714)
                     /locus_tag="PKM72_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59732.1"
                     /translation="MRGRFASIALGLALVTWTLLGHVAISEADVVFLENGDRVSGDLI
                     ALDDTVLAIDTDYADIVNIDCDDVAGLTPDSPLWLAFHDGAIIPDGLGTRDGDRLILP
                     A"
     gene            complement(27920..29395)
                     /locus_tag="PKM72_02770"
     CDS             complement(27920..29395)
                     /locus_tag="PKM72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HNP59733.1"
                     /translation="MTTLIPKLQKPTWLACLALSILILIPGSELGASQESENQLFSPK
                     FPQALKPWTGDWDGMVSRNQIRVLVPFSKTYYFLDRGRQRGLTYDLMKIFAKQINEDL
                     QRKIVQVQFIFIPVNRDELIPDLLNGVGDIAAGALTITPERKKRVDFSDPLLTGVREI
                     IVTGPSSPPLSSLDDLAGKSIHVRKSSSFYEHLVRLNASFATTGKPEITLIPEEENLE
                     DEDLLEMVNAGLLPMLVMDSPKAEFWAKIFEHIRLHPDITLNAGGEIAWAFRKNSPKL
                     KRVINRFVSKNKKGTLIGNLLFTRYLKNTRYVENAVSTQERKKFQNLMHVFKQSARPY
                     GFDWVLAMALGYQESMLDQRKRSSAGAIGVMQLLPSTARDPNVNIPNIKQLEPNIHAG
                     VKYLHFLHDRYFKDPKISLLNQWLFAVASYNAGPARIAKLRTKAPSMGLNPNQWFKNV
                     EIVAAKHIGRETVQYVSNIYKYFIAYRLILEKEEKKSTVQY"
     assembly_gap    29582..29681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29948..30646
                     /locus_tag="PKM72_02775"
     CDS             29948..30646
                     /locus_tag="PKM72_02775"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNP59734.1"
                     /translation="MPRSERFLLALVFFAMSACATTQQANTVNRSGFLEDYSMLQKGE
                     GDSEALLRYVNPVADWKHYSKIMIDPIQLWLGEGSSLRDIPQEDRIRLTSLLSGKLQN
                     ALLADYRIVRKAGPRVMRLSVALTEAEASNTVLDTLSSVLPTGYVISGTKSLTTGTGT
                     FVGSASIEAKISDAELGTLLAAAVDRRGGAKSLSGVTSEWNDVEESFEYWANTLRYRL
                     CQWRGEHSCVPPTP"
     gene            30942..31748
                     /locus_tag="PKM72_02780"
     CDS             30942..31748
                     /locus_tag="PKM72_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59735.1"
                     /translation="MIGVILGFFGSQSTHAEEITKLAEEVQNPVSDLVRVGFVNGTLF
                     GAGANNHLVNVFNLQASTTRRFGNWALLNRLTIPIPYIPANAIEDKTGSMTGLGDIEY
                     TGFFARDESKRRFKLIGGIGPTFILNTATDNHLGLGKWSVGPTLALVSIPDPWVVGAV
                     IRNVWSFAGEKQRPKVNLFTLQPFVNYNFSNGWYLTSTPAITANWEAEDRRNRWTVPL
                     GGGFGKVVFRGEKRPINIKLQGFYYLEKPDRAPDWTLQLQFQILFPDKPA"
     gene            complement(31891..32451)
                     /locus_tag="PKM72_02785"
     CDS             complement(31891..32451)
                     /locus_tag="PKM72_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59736.1"
                     /translation="MRTGMIALGIFGLVSLQMGCAALPGSDLLSSKANGTKTVTTDQQ
                     FDELADNSSSFLNEEKKLAGAVVSIDQTDEGYLILAEWLPYPKTELAEGPKIGQSDMS
                     RHFLIRFIGKREKEFYTTRGNIFLLEGTVEGTKKALVNVFGPRKDLLYVKAKCIRIWD
                     TGQDRDPSSQLDSQYPPRISRTLCAD"
     gene            complement(33376..33900)
                     /locus_tag="PKM72_02790"
     CDS             complement(33376..33900)
                     /locus_tag="PKM72_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59737.1"
                     /translation="MRVQRSCIILSCVVMLAGCVGTQQGGRQPSGSSVDSAPPPQART
                     FVFPEKGQSPEQQEQDDFTCYKWAKQQTGYDPEHPNLAAAPPPPSGGGALFGAMGGAA
                     LGAIGGAIAGDPGMGAAIGAAAGGGLGMMGQRGAEQEYQQQVQQAGAQNQRTRETFDR
                     AHRTCMEGKGYKVG"
     gene            complement(33935..34405)
                     /locus_tag="PKM72_02795"
     CDS             complement(33935..34405)
                     /locus_tag="PKM72_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HNP59738.1"
                     /translation="MRTYMVLGLALGFGMLLHVSHLSAQTFVFPEKGQSPEQQELDDF
                     TCFKWAKQQTGYDPEHPDLAATPPPPSGGGAMRGAMGGAALGAIGGAIAGDAGKGAAI
                     GAAAGGGMGMMRQRRVERGYQEEVQQTSAKNQQTRATFDRAHNVCMEAKGYKIG"
     gene            complement(34443..34988)
                     /locus_tag="PKM72_02800"
     CDS             complement(34443..34988)
                     /locus_tag="PKM72_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59739.1"
                     /translation="MKRLVSGIITMTVLIAVTLVLSVNGYAEEGSVKAMSPWKGVGFA
                     FPVGPDQVYMVAVYSGVMLMDDAKGALHAASIVCPGTVDADMKKGTKTGYAKCVITGE
                     QGNRVFAEFNCTGDVEGCRGIFKITGGTGKFAGITGEGEMISKIQAREITMVSGVESA
                     QQLGEGVAVWPNLTYHIPDQK"
BASE COUNT         8646 a         9555 c         8707 g         8537 t
ORIGIN      
        1 tcccggcccc ttcccacctt tctgattgtt caaactgatt agatgccggg tttcgacccg
       61 gcagccgaac cacttttgtt tcggcaaaag tgctcaaaac cattgacgcc ccgtttggcc
      121 tcattaaaga ggagggacgc caactctgaa gagcggccca actcgccagg ctcagacaag
      181 gtccactaaa tactaagagc gccccttcct agggccagcc ggaaggcgtc ggtcaaaaca
      241 aattcgcgcc gaagcagatc catggaggac gacaaaatcg tggagcctgt cctgagtttt
      301 cgaagggctc agacaaggtc cgctagctgc caagagcgtc cctcctttgg gccaaccggc
      361 aggcgtcgga tcaactagcc acctggtggt ttgagcgtca gtccggtggg ccgaccggaa
      421 ggtgggaggt taggtaaacc ttctttcaaa ttcgcctgcc agtggctctt tcgcctttaa
      481 tctgaacagg gaaatatctg gtagaaaata gccggcatga cggagaagag ccgatgcagt
      541 tccgctaatt aaaggcggga atggcaccca tggagaaaga tggatgttag gatagagtca
      601 ggttgccatc caaaacatgc ggaaattctg ctcaaaaaga tgggctaatg ctaagctgaa
      661 ccgtttttgg taaggttccc tgagaacgat gacgatacac ggttttagga ctttgatggt
      721 tgtcgggatg cctttgcatc atttttcggc aggcagggaa cctggtttta tatggaggca
      781 atgatatgaa ccacacgaat cttctctcgt tattaacaaa tggccgtgag gaaatcgtac
      841 gccgatttgg catcaagaaa ttgggcatct ttggttcggc tgctcgtgat gagatgcacg
      901 agggtagcga cattgatgtt ctcgtggagt ttcagtcctc ccccaccttc gatgcctata
      961 tggatctcaa gttttatttg gaggatcttt ttcattctag tgtcgatttg gttattgagg
     1021 ataccgtgaa gccacgtatg cgacctctta ttgaaaagga tctgattcgt gtcgcgtgat
     1081 tggacgttaa ccgtctcatt tcctttcaaa gccctcatat gaggtcttcc atccttccct
     1141 accgcaagat ttcctttaaa ttccctgtcc ttcaaaaaca ttccaccttt ctttggcgat
     1201 tcttctgcga gagtttgaga gtggataggg tcagccctcc aatcgcagat aatccaggcc
     1261 ccttcccacc tgcctgattg ttcaaactga tttactcttc tctgcggggc tttaaggtgt
     1321 ctcccgaaaa agatggatgg aagttgcctt atagatgccg ggtttcggcc cggtagccga
     1381 accacttttg tttcggcaaa agtggtcaaa accattgacg ccccgtttgg cctcattaaa
     1441 gaggagggac gccaactcta aagagcggcc caactcgctc cgctcagaca aggtccgcta
     1501 gctgataaga gcgtccctcc attgggccaa ccggcaggcg tcggatcaac aatttgcctg
     1561 tcgattctgg cgtaagcccg tggggatgac ctgaagatgg aatcatcaag gtttcggaca
     1621 ataacttggc ccatcgtctc cctcctttgt tgttagcaaa aaatgatatc gctcttaagg
     1681 ttaaggggca aggtttttcg agcaaaacca cgccactgga gagataggtt tactcgaagt
     1741 tcgagcacca tcaaatcttt tcagttgaaa tggccatcct cgacaacgca atgtgcttga
     1801 ggggcatgtc gccctccaac attccgttgt cattctgagt gtaacgaaga atctggggcc
     1861 agctcacagc cacatggctt agcgaaatgc cccccgcgac tcatggcttc ccgtttgagg
     1921 ccgctttcat ggaaagggaa atgcgcttgc ggggaatgtc gacttccagc acggtgactt
     1981 tgacttgttg gttgacctgc accaccgtgt tgggatcgct gacaaatttg ttggcgagtt
     2041 gactgatatg cacgagccca tcctgatgca cgccgatgtc gacgaacgcg ccaaaggccg
     2101 tgacattggt caccaccccg gacatgatca tgcccggctt aacctgctcg atggattgca
     2161 ccccttcatc aaatttcacg gcttcaaatt gttggcgggg atcacggccg ggtttggcca
     2221 attcggtgag aatatccgtt aaggtcggtt tgcccacttc ctccgtgatg tatcgagtga
     2281 gatcgatggt gcgttgtctg gcaggatcct tcatgagatc cttgaccgtg cagcctaaat
     2341 ctttcgccat cgcgttaacc acggcatacc gttcaggatg cacggcgctg gcatccagcg
     2401 gatgctcccc atcggtgatg cgtaaaaatc ctgcggcttg ttcaaacgct ttggctccca
     2461 ggcggggaac cttctttaat gccgtccggc tcgcaaacgg accatgttcc tggcgatagg
     2521 ccacgatatt cgtggcgagt tgcggaccca cgccggagac ggccgtgagc agttgcgggc
     2581 tggccatatt gacgtccacc cctacccgat tgacgcaact gatcaccaca tcctggagcc
     2641 gttgctttaa gatgctctga tcgacatcat gttggtattg cccgacgccg atggccttgg
     2701 gatcgatctt caccagttcg gctaagggat ccatcagccg ccgcccgatc gagaccgcgc
     2761 cgcgcaccgt cacatcgtga tcgggaaatt cctcccgggc gaccggagac gcggaataga
     2821 cggacgcgcc gctttcgttg accatgacga tcgggatggc ggatggaaat ttgagcgcgc
     2881 gcagaaacgt ttcggtctcc ctcccggccg tcccattgcc caccgcgatc gcctcaacct
     2941 gaaaacgctg acacaactcc accaccgtgt ctcccgcctt ggcggctccg gctgctccca
     3001 gatgcggaaa gatggtctcc gtatgacgca aggtgccctg tcggtccaaa cagaccattt
     3061 tacatccggt gcggaatccc ggatcaatgg ccaggacggt cttctgtccc agaggtggcg
     3121 ccatgagcag ttgctgcaca ttttgggcaa acacttctat ggccgtttga tcggcccggg
     3181 ttttggtgac caagcgggct tcggtttcca tggaaagggc cagaagcctg gtaaagctat
     3241 ctttgacggc caggatgacc tgttccgatg cgggcccctt tcctttcaca aagaggcgat
     3301 gcagaatgga gagggcttcg gcttccggca ccagcacacg aaaggtcaga aacccttcca
     3361 cctcgccacg ccgcatggcc agcacacgat gggatggcgc cttggccacc ggttcttccc
     3421 attccacata atcccgatac ttactgcctt gcacatcttt tccgcgcaca gccgtggtct
     3481 tgaaagtccc ttgttccaga tacagggcac gcatggaggc gcgagcctgc agatcttcgc
     3541 tgatccattc ggccatgata tcgcgcgcac cggccaacgc gtcctccacc gtctcgacgc
     3601 tcagttccgg attcaaaaat ttctgcgctt ccgcttcgac attcaattgg gcatcttgtg
     3661 cccagaggct caacgccaac ggttccaatc ctcgttcttt ggcgatcatg gctctggtgc
     3721 ggcgcttggg tcggtagggt aaatagatgt cttccaattc ggtcatggtc gtggccgcct
     3781 gcacctgctc ttgcagcaca tcggtcagct tgccttgttc ctcgagcgag gccaggatga
     3841 cgtcccgccg tttatccaat tctcgaagct gcgccacgcg gtcccgaatg gaggtaatga
     3901 cgacctcatc caacccgccg gtgacttctt tgcggtaccg tgaaagaaac ggcaccgtgg
     3961 cgccatcatc cagcagactc accgtggccg tgacctgctt gtcggtgatc ttcaattcct
     4021 tcgcaatggt cttatcatgc aatgctgaat tcatagtctc gttacatcct tacaaatttt
     4081 tgagtgaaat ttttgtcacg agtcgttctt cacaacgaca gcccggttgc gcttacatcc
     4141 tctcagcaac tcacttgggt tggggttcca gctcccatcc ctttgctcgc aaacactggt
     4201 caacttcttg ttgtgccgac tgctcggccg atcgagtgat gaacccttgg ctgacccctg
     4261 gttttcccat ttgaccactg ggaatgcccg ggccggggga cggcatcagt atttgactgc
     4321 ggcaatcgac tcgatcagcc tctaagtccg cttccgtggc accggccttg acatacttca
     4381 cgtcagtgca tccatagagg aacagacttc caacgcaaac caccagccat gctctcatca
     4441 tgagataccc tctttttcta aatggagttg agtggaaatg tggccttgat atggtcttta
     4501 tggaatagac gagatatact cgtcaacttt caatctcaaa aggaaatgta ggataccaga
     4561 gtgaacacct tttcaaaaat acagaggctg agtttcctct tctgtgtgat tctcagtctg
     4621 cccggttgtg cgcaaaaaac ccaatcattg gatttttccg gttttttaac tgattacaac
     4681 agcctccgtc cttccccgga tgacagcggt gcctggacct accggaagcc aggcgtcaat
     4741 ttcaaagact acaacaccat catgctggat cccctggtga tctttcccag ccaacattct
     4801 gagtatggtg gccttgatgg gttaacagcc tggagattgg ctctggcatt ccaggaaagc
     4861 atgagccgcg cactggcagg aggctatgtg atcgtgaaag atcccggtcc cggggttctg
     4921 cgcctccgcg cagccctgac ggatgtgatg ttggagcgcc cggcactgga atcgtcgatc
     4981 ccgctgcttc ccctggccaa cgatttcctg atccagggtg ccgaaaaaat ttccggcatg
     5041 aatgccctgg agggagaggc ggcgattgag gccgagatat tggatacaca gagccaggaa
     5101 cggttggttg cttatgttga aaaacggatg agctcagagg ttctcctgac gcgagaaaag
     5161 gattccctgg gacccgtgct ggaaatattt gactactggg caaggaaact tcgacaacgg
     5221 ttagacgagg aacgcgggat acgcgaatat caaaaagata ttcagtaaac ctctctccag
     5281 tttcccgatc accacaaaaa ctaaggggat aagaggcaag acttccacga tcacggtcgc
     5341 cggggtgaag cacctatgcg ctggatcgtc atggttactc tttaactttt agacaacgct
     5401 tcttctcaac cgagagttgt tttttccctg tccgggggtg gtcttcatcg ttgaacgcat
     5461 gatcatcctc tcgccacgtc ttacctgttt tccagacgac tgtagtccag caaccctgct
     5521 tcaacgggtg actcgttttt ataggcagct tgtatggcat cactatgctt ccaaaaatca
     5581 ggatcacttc gtctgacacc gaaacgattg agcagagctt ggtaatcctc ctcactcccg
     5641 agatgttcca cggcgctgac aaactcttcg agtgtggatt cgtcgacctg gtaaaaagcg
     5701 ttgggatagg cacccaaaaa ccctcgcacc accgttaagg tatcttcttc cggcaaacgc
     5761 cgttcttgtt caaagagcag atgggacaca ttgctcatcc cgttatcatg gatgagggtc
     5821 accacctgag catgggaccc gccaattgat aagatggcgt tttggggcaa ccaggacacg
     5881 gctgttcctt ggaggtggga taacttctcc agctgttgac ggacgtggtc attaccagaa
     5941 ttctcaatcg catagggcct gtgcaagacc ggcgcgagcc gttcgcgcac catcccgatt
     6001 aattctaatt tatggtctgc cgtgcgatat tgaattcctg tctgacgatt gatgctgttg
     6061 aaatataccg caccaaaatc ttcgacctta tccccggcat ctcgatacca gtatttccat
     6121 tcttgggttc tcgtccgttc cgggagcagg gtgagaaaat ccagttcccc ctccattcgc
     6181 aaaaaatcca tgtacaggcg ggaatttaac tggtgaccga tattgccata cacgtcatac
     6241 cctgcaacca acagataatg aatccgctca agcaagggat accccagtaa caccatcgtc
     6301 ttgggctcct cgccgacaaa ccctttcacg acggtcgcat tatccagatg tcgaaacaca
     6361 gtgagagccg cattgtcatt ctgcccatcg ccatcccaaa gcaggtcgag tgaaatggct
     6421 tccggtgtcg agacatgttg ttggatgaac ttaattttgc cggccacata tttcttttct
     6481 aattgagaat atttcagcca ggtccccagg ggtgtggcgt tgctctgctc gacgatgggc
     6541 atttgaagat tcttcgcttc tttttcaagg aattgggccc cctgggcaat ggtcgtgcta
     6601 ttggggtttg aaaaaactat ccagaaccgc tcattaatca cattgagcgc cacctgtccc
     6661 cggcagacag gtcccttgat gaaattcatc agggtaaact gggcttcatc gagcagaaac
     6721 tgataccggg atttcaccgg aatcaatttg aaggtttcaa aaggattggc cgacgtctcc
     6781 gggtcataag acggcaaggt cggcacccca taggccggtt ctaaaaagag ggtgcgatac
     6841 cgggacatcc ggtcgggatt gagcgcatac ggcatgtggg ttttggccaa aatcgtggtc
     6901 cggaccggct caaagcgata aaaaaaacga tccactttcg ggtcatcata aggccgtctg
     6961 gtggcaatga gatcaatgcc ttcacctgga ggggttgtgg atcgtacgag acgaaagaac
     7021 tgcttggggg cgaggtcatc aaaataaaga tgggccagga agagatgctc gtagagatac
     7081 cggctcatta attgttcttt cggtgtctgg ccattcaaaa acgtttccca ctccgaaatg
     7141 tttgacagga cacctgcggt caccggagct ggtgcttcat cgtcggcgcc ctcctccaac
     7201 cacttcatca atgtggcatg ttcctcatcg cctaaaccgg gtaatccaaa gggcatcccc
     7261 cataagggat tgtcccgcgc aaacgcgtca aactccggaa gcgtcgggca atattgaggc
     7321 cgctcgatgc caaaatcaaa actggggggg agcgggctga cagcgggaag cgggtgctgt
     7381 cgcttcagga gcaacatctt cgccatcagg ctcgcttgga gattggcctc ttttgtctgc
     7441 acccgttcgt tcaacaccgg aaaaaactgc ttctcccgcc attcggaggg tgaatgcgca
     7501 tcgacaaaaa gccgggtggg ttccatggcc aataatcgac cggggttata aatcagttct
     7561 ttgttggcac cgcgttcaat cccttcatgg gcactgagat tgagttgaca tggggcatcg
     7621 taacatccat gacagacgat acatcgggcc tcaagaatcg gtttcacatc ctgccggtaa
     7681 ctgactgtgg tcaccccggt tgtttttgtc agccggtttt gcggggagga aggcccgaat
     7741 tgttgttcca gctgcatccc ggcatagagg gcgcaaccac ccacgatgat cgtgagaagc
     7801 aggataaaca caatacggac gtatggacga tctgtcatct tatctcctca gggttgcttc
     7861 tctattgttc aggaccatgt ttttcgttca cggtccccca ctatatctaa cgggtagcga
     7921 agttggcgaa ctttctgaat ggccgttatt tcagcgcagg atatggtgaa ccggtgagcg
     7981 tcgttttttt ttaccatggt cccggctcgc ataaagagcc agaaacccat cacgaaagtg
     8041 ctcgtttgtt atctatacaa aaggaaaaaa tctgttgatc gattggacgt tcgtcaacca
     8101 cgtggcagga gattgtttca acatgaagat ggaaatcgac tgaagaggaa aacccgaaag
     8161 cgcctcggag ccatcgcgtg attggccttc ctgttcacat gaggcttcaa gtgaccaatc
     8221 ctcgcaccct tcacccgatg ccaacgcaat gagtcagagg aatgggttct ctcatcctgt
     8281 catgatcgaa gtgcagggag gaggcatcgg tcctgagccc ggcaacaaac cattccccct
     8341 cgaccgtgtc acagatgctc gccagaagga ctgtggagtt aagctcctcc cggccccatc
     8401 ttcacccctt ctccccaggt gacggtcaag gcaaaaaagt tgagcgtcgt attcttaaag
     8461 tcaccggaca cccggccgga aagcggtcca cgattttgat caaccggcat atctcctatc
     8521 caggtcagtt cataggaaaa cccaagattg agggtgcgcg tcgcttgcca gagtgcccca
     8581 aacccgaatt tgtaggtttc accaaccggt atactgagtg tacgattttt atccttcacc
     8641 atggaactat cgtaggcaaa accggtcgag accacccatg cggggttcac acgatattgg
     8701 gtcccgaatg ccacatgcca ggtatcctgg tagttcaggt tggcgaccag gtttgtggga
     8761 ttggccgtat ctacgccgat atccaccttc ccgaattcag accagtcctg ccaaccaaaa
     8821 tcacccatga tagcccatgc cccggtcagg tcatggtagg cgctgaacat caacgactgg
     8881 ggaaccgtca tgccgacatc gagtttctta ttcaacaggc cgttattctg cagagcggtc
     8941 ccgatgggtc cgagattcga aaacttcggc ttatcggaaa aattcaagtc aatgggagat
     9001 tgatacgtga gtccgaagcg tgtccctcgg gtcggcgtga acaggacgcc gatattgccc
     9061 ccaaccccga cagcctgatc tttgatttgc atttgcccgt cgttggtact cccgagattg
     9121 ttgataccaa ccgtttgttt gacggcaccc agcatcacgt tgacccctcc gccgacagac
     9181 aggtattcat tgacacgata gctggccgtg ggcataaaac tgactccggc cagcagcgca
     9241 tctttcacgt agtagcgacc gacccattcc cctttgtatt tcaatcccag accgaaattg
     9301 gaaaatatgc caaagcccac tctccaatca tctcccaggg gatgcacgta aaatccactg
     9361 gctccgggaa acacatcaat gggattcccg cctcctccgc cggacggggt ggacttgccg
     9421 ttggaggtga atcctatatc accatataac agttgagcgc caccctggaa ttgagcgccc
     9481 tccaggaggg acatgccggc cggattctta aataaggttg acgcatcctg agcgcgagcc
     9541 gcccatcccg ccccggcgag tcctacgtcc ggtgtgccga tttcagtcag gaagagacct
     9601 ccggcttgaa ttgatggagg cagaatcaat gctcccatca agagaagacc caggatgtgt
     9661 aaccgggctc tcaccgcaac aacttccttc atgattcttc cttcatctga gggatgacct
     9721 gctggaatgt ccagggccac acgtttgatg tggccctgaa atcgtttatt caaattggac
     9781 gtcgagaatc tttcgatccg catcctggcc ggaaggcagg acggcgcatc cgagatacac
     9841 cgtttccttt gctttcaccg ggatttgggc ggtcgtgacc acgttgtctc ctgtgatttc
     9901 ggaaacggtt gccatgatgg tcgcatccgg atgcttgttg acaattccct gttcaatctt
     9961 ggaatgagga cactccccgt taggacgctc gatcatttga gcatacggtg tggcatccat
    10021 atgctggaga ttccctccac cacaggacgc taaggcgagg atcacgcaac tggaccacca
    10081 gggcacgact ttcataggaa tctccttttc agtgaacaac gggatgattc gaccggatca
    10141 tgctggggat gccgcgccat tacgcctccg gctccacaca attttctcca cctcgaagca
    10201 gacatgcccg ccagcgaact ttttccgccc aataccggta ggcttcctcg acatcgttcc
    10261 acgaattggt gactccgctc agacttttcg tccctccccg gcgatccacc gcagcggcca
    10321 gcagggtttt gtccgaggaa tccgtgactt tcaactccac actcgccgac cctgtaaaac
    10381 cggacacgcc tgtagcgaaa ccttttactc cggttaggac tctcaactga ggaacgatgc
    10441 ttgaaaccgt atctagaacg ggattggagg tttcggcttc cgtaatggcg gcctggagac
    10501 gcataacatc cggaccgggt gtggtcacaa tttcataatc ggtttttaag gcttcatgca
    10561 atttggccca gaagagatcc gccatccgtt gacgatcttc cgcggacacg tctttcaatt
    10621 gggagtcctt gccgatccag agggtgacgg gatcaagaat gattttctgg tattttctcc
    10681 aattggcttg aggattttta tagatcaaaa gggtttcgtc ttttttccct tcttttaaca
    10741 tggaatagtc tccaagaaat cctgattgtt ccacgctccg agtttgctga gtgctggcac
    10801 aacccgatac acccaccaac aggatgagaa ccacatgtaa cggtaaacgg atcatcacgc
    10861 ttttctcctc gcttaagatt gataagaggc tcgtcaaagg cagttgtctt tatcgggatt
    10921 atagcgatat catcatggaa aaaatacgag gaataacagg cacggttaac gtgttgtatc
    10981 atgccgccaa ctgaaaaact cctcgcggat cggtaacctc gaaaaatgtt ggctcaggaa
    11041 gatgccaggg cttatgaaca gaccctcgtg caatcctccc acccccccac atcaggcgca
    11101 gaggccatgg gtcttgcagg tttccaaggc atgaagcaat aaaatccgtc tgacttcctg
    11161 gatcgtatgc gtattcgatt gacaggaatg gccggaccgg acaatgaatt ccgattccat
    11221 tccctccaga tgagcactct gatacgccac cactccatcg ttcccatcct ccatgggacc
    11281 atcgccctcc accgctataa ttgagtggcc ggaaactccg ggagccaaag gggtctgagc
    11341 caggcctttc gccagggggc ttcccggttc catcgccgac acacttccca cattcattcc
    11401 ttgtaaatcc agcttgagtg tgtcgaggtt taacgacagg agatctgtga cacctgacat
    11461 caatcggccc ggcagcttta cgaacttggc cagttgatgg gcaccccaac taccagccac
    11521 ataactccca tgatggggcg tggcaataaa gataaggcgt ttgaccgtgg gaagtggggt
    11581 aataaacagc attcgttcca gtaagtcccg ggtctggtcg gataccttga tgtcgtcaag
    11641 tggttggttg ctcacttcat cccaaaagat atttccggtt tcgacggccg tgagtctgct
    11701 caacagccca ccctgactat gtccaaggac gaccatcgcc tggagagcag gatcctgata
    11761 tttccgctcg aggtcactca cagttgtttg cagggtttcc cgcagcaaag ccgcagaata
    11821 caaaatggga ttgccggtat catatgtgaa aaaccagaac tgaaatcgat cacgaactga
    11881 ggggtccgcc atcagatcgt tggtcatgtc tgcccacctt ccggagccgg aggccgtccc
    11941 atggacaaag acgaccggaa tacggccggg tctatacggc tccagagcaa ttaattcccc
    12001 tgactcaccg gccaacgaat ctcccataaa aaaccccttc agttcccgtg cccagacccg
    12061 gtcatcggcc aaactagctg ccagagaaga ggtaaattca atttccaacg gcacatcact
    12121 ttcctggata cgtaccgtcc tctggtcata ggcgctatat acctcccagt ctccctgaat
    12181 agtccccttc ttcagttgtt ccataagatt gggaagcctc atgaaggccg tgacaggcat
    12241 tttgcctttg gcaatctgta acccttcctc ctgacctaag gggatttgat cggccgctaa
    12301 cggaaccccg attccggcct gccgataacg gttatgtaac ccacggactt ccaactctgc
    12361 gacggggatg aaattggtga gagtccggtt ttcccactgg agccgatttt ccggaagggc
    12421 cacctggagg gttccgaagg gaagggtata gcttcccgat tgcagaatca tgacggaacg
    12481 ctctttggaa atgaatcccg tcgtcagact cctattgtac aaatcagccg caatccggac
    12541 acgaggatca aatgggttga aatgattgct cgaatcttcg ggaaacaaat agagatacgc
    12601 atacaaggcc gcggccagat aataggtcgg ttggttggtt ttatgcgcat agagaaaaga
    12661 caattcagcg agcgcgaacg ccacgggacc ttttcgttgc tcttcagcga ggaagatatg
    12721 gtgcaaggtg ttcaacgcct cacggggatc atcaaaataa gcctcagtca gatcggtgac
    12781 acggagcaca ttttcggtat aggggctggt ttcccctgaa gaaatgacgt ttttggttaa
    12841 ttggtgccga acggtctcag aatcgacacg agtcacgcct acaggcgact ggcaaccggt
    12901 catcatgacg gcggtgaaga tgataaaaaa ccagaggccg tgagattgtg tacgtgtcat
    12961 gttccttgcg attcctttga taaaatcggg taaatactga ctccggccaa acataaatca
    13021 actttttctc cgaggtgggg caactgttgc caccctcggc ccaccgactt actcctccat
    13081 tcacggagga gcaagtcggc ccaatctgcg aacaaccgag gaacacaacc tataccatct
    13141 tgctaaggca caattcagga ctcattgctg aatccccgac taatccatca atggaatatt
    13201 ggccgcgaga tcaaacttga tcgcagcccg gatcttattc tccatttgca gatagcgagc
    13261 cactttatga gcgggaagga ctgcgctgag tttttcagca tattccctcc gcattttgac
    13321 ttccgcccct tccgttttaa ttgattcgtt gatgagatct ttggccacct tatcaggcac
    13381 ggatccttta ttgtaggctt ccgcatattc gttgatgagt ccgccaagat gctggtttaa
    13441 atgctccagt tctttttgat agccctcgta gagtggccag aaatccttgg attctttgtc
    13501 cgtcagatcc atattgctgg cgatgagcag cttcttatcg gctttgacct tatcgatcag
    13561 tatttgcata ttggtgattt gtgcattctc cgcgaaaact ggagtcacgc acagaatgac
    13621 ggcggcaacg gtacacatga tgaactgctt cataacgcta atactccttg gttgtgggta
    13681 gatgatggtg aatgctagat gagagaatga aattctctca catcttcgcc tgaatcggcg
    13741 gcagcaagcg tagcaaaggc ataaattaaa gtaaacgcta gagaaaagta tattcagcct
    13801 gtctgtggga actgggaaaa ctgaaatagc acaacggtta cagattgaga atcaacgaac
    13861 atcctggttg cagatatcag ggagagagaa gatgggcacg gatgcgactg gaaaaatcag
    13921 gagcttggtc ggcctgcccg gcccgttcgt taatatgggc acgagcctgc aatgcttcga
    13981 acgaaggacc ggcacccaac tttccctgtt cgtggaggta ggcaggagtg tatccgctta
    14041 acagaatttt ccaggaaaaa ggcacatggc cggggttcag acgcgtgtgc atccagatga
    14101 cattcgtgca gttggcggtg agggtattgt agaacttcgg tcgctcgcgg agttcgttga
    14161 tggtcttcat gtaccccaga aaaatccgtt tgaggttttc aggagacccg gttactgaat
    14221 acaggtacac atcttcagga ggatcttttc gataattcgt ccggacccgg atcagatccc
    14281 gttcatcgcc cacgatatag atcagctcat actgtttaaa aaatcctttg atggaagaat
    14341 agccttcagt ggcttccttt ctggcttcga tggaaatggc caagtggtca tcaccgaatc
    14401 cgaacgtgag aaaaatatgc gcgatcatcg gacccatcca ataggcggcc acaaggtcca
    14461 ctgaatccag tttggtgaga tcgtaggtct ggtcgtaata ggcgggcgta aagtcggttt
    14521 cggtacggta gtcaaagttt cgaatattgt gaacggtgat caaatttccg tcgaaggtgg
    14581 catagggaag catggccact tccgcttgcc acacgcgatc attggacggc tcgatcatgc
    14641 tccaccagac aaagagcagg ataaacaggc tgagataaga cccggcggcc atccatcgaa
    14701 accgaccggt gcaaaagccg accaacgcca caaggccggc tacggcaaag catgcagcca
    14761 ggagcgtccg gagcatgtca gatcccggcc cgtcaaaata taacgcgaag gtgccccagc
    14821 cccccattcc aagaatcagg aatgctccca gagaaagacc gacgtacctt attatctgtc
    14881 gcagaaaaat tcctttagga gcatgtgtca gaaatgaagt gcactttact cttgctcttg
    14941 gcaaaaccta ccaaacaaga gacatgccat tcgcgagcta agcagaagat tccggaagat
    15001 tgacataccc gggaaacgac aatcaacagg ccgggtgtca tctggaatta tcggatcttt
    15061 gtccgaaaga tactgcggca ggcttcggga aatttttctg gctcattgaa cagcatactt
    15121 gaaaacgtga tcgagacaac acacatagtc atcaattccc agaagttatg caatgggagt
    15181 caaatatggt ttgcggaaat atccaatttc tcctgtcata tccctcggac ataaactcaa
    15241 aataaatccg aaagacccgg accttctcac tatcatgaag atgtatcttt tccgcgattc
    15301 atcgactttc gggaaaagtc acgcctgaaa aatttcgaga tcacatctga ataacacgct
    15361 gaattgtctt cggctctctt cctcggaggg gggaattcta tcagaaccat cgggaatgga
    15421 ctttaatggc agttgtgctg atgtgaatgg gaatttaaaa tttggtgccc acatttattc
    15481 ccgtttgtgg gctcgacaga agtagacggg agattctttg ctggtgcggc gtaaccgcac
    15541 tacaccatga agggaaaaaa tctatgccga ttgtgttcag caggaggttg cgaatatccg
    15601 gcctcatcct actcgttatc gggtttatgt cggcctgctc aactctccct gggaattttg
    15661 agacaccaaa gatctctatt gccggcatta ctccgaaaga tgtcaccctc tttgaacagc
    15721 gttttgaggt gcaactccgg atccagaatc ccaataattt cgatctgggt ctcaacggta
    15781 ttcgttttga tattgagctg aacgacaagg aattcggcaa cggcatgtca ccagcaaaag
    15841 tgatcgtccc gcgattggga tccgaggtgg tcacgggaga ggtgattacc ggcctcgaca
    15901 gcttccttcg tcaggttcaa ggaatgaatt ccacggccgc caaactgcgg tatcatctcc
    15961 aaggcacagc cttcgctgaa tcccccggca acttcaccat ccccttcgac gatagcggag
    16021 aagtggacct ccattttgcg ccggcagcac aagagtaaaa aataatttcc ggttttacca
    16081 tgtaaaccgg aggaacccac cgttatagtc aggagcattg atggaagggc ggagcagaat
    16141 gggttgcttc tgtttgacat ccaggccctg cggcagattc tctgacggaa tgacggattg
    16201 aaaagcctgg aggctgtgca tttcgacccc caattcaatc cccggcaact tgccgggaga
    16261 tttcagttga agacgagctc cattccccga ttgctgcaat accccacttt gaagattgcg
    16321 ttgaaacaat ctttcccctc ttaccttttc ttccgcacct tctccatcgt tattggataa
    16381 attgggatag gatcccccaa tttcccgagg tggatgcacc tctgccatca acacctcctg
    16441 ccccttcacg gcagcttgga cagatgcacg gtccagaccg ccctgaagaa accctgcaag
    16501 ggacataatg gtggcgagta ccactctttt acttatcaat gtccgcataa atctactccc
    16561 ttgttgctct cccgtttatg atgactattc accaatgccg atacttggat gacctcacga
    16621 ttatcataaa agagtttttt gttcatcccc gcatcctggg gtctctaccc cccaggatga
    16681 ccactcaaag catgtttgta attgacacgt tgcgactcca gaaagattat cactgaacat
    16741 ctttgggatg ccagcgaagg accttgaccg gtgcagcccg tgggaaatct aaaaacccct
    16801 gttgccattc tgagaatccc ttccaggcca acaatttttc ccgataggca ttcccttgcc
    16861 tcgccacgtt ggccaccgcc cccttaagat catggacctc ctgtttcact tgccccttaa
    16921 tatccttctt catatgctca tacccacggt gaattttttt ccgggttcgt aaaccggact
    16981 gcggagccgc aagcagtcca cctatgagac cgaccaggga gctggtgaca aatacaagcc
    17041 ctgagacctt ccacatgact ttatccattg gacacctcct tgaatggtaa acggttcata
    17101 accacatgct tcgtttctaa tccaaatcag gtttgctctt acaacaaaaa gaaaagcagc
    17161 cggcaatgga tcctccctta ttatacaatg tttttcttcg tcaaattttg acttttcgtc
    17221 aacagagaat aaaaaaagag ttgcctcagg cctttcctac ctgagcggcc tcctggggaa
    17281 acacttcctt caaaatccgg attctggtct gctcataatc caattcgctg gacaatggac
    17341 gccagccgaa tccatcaagt aatgcatgca tattttccca gactgctttt ctcggatcgg
    17401 ttattcccag agcatgaagg ttcacttcgg aggacattaa cacgtatgcc cccaaggccc
    17461 ctgtccataa accaaacgcg acttcctcag gggtgatgcc ctctttcaat tgaacatgtc
    17521 cctccgccac cgcatcacgc aacacgccgg ccacgaggcg catgttttcc tgattcgagg
    17581 catccatttt tttttgaaaa gtcggcgagg cctttctcat gattgattca ttttgaacca
    17641 ggagtttcgc tttaaagtgg tccgggttca gacacacaaa gaggtcatag cccaacgcca
    17701 tggctgccat ccgttcacgc ggattgcctt tcaggcttga acccctgaga agaaactgcc
    17761 cggctttatc ggctgactcg gaaagcatcg ccactaaaat ttcttccttg catgagaaat
    17821 gttgatagat ggtacctttt gaatattctg ttacttcagc gatttgatcc atattgagtt
    17881 ccagatagcc acggtcgagc aatagctttc tggccgtatc aataatcaga ctctctcgtt
    17941 gggcatattc acgtttcttc cgttctgaca cagccataag tcatttgtag caaacatttg
    18001 acgtttagtc aatttttttc aatgcgtcac aaaatgactg ccattcatta tataagaagg
    18061 tacatttcac atggctgttg aaattcgctc aaatgctatc tgtgtaggga aagtatgctg
    18121 gaagggggga gagggaagag ctgtggatat tctgaggggg catgccttat cccgaaaaca
    18181 ccagaggcat acttccccta cccccatagg aaccatgagc gatcgccatg ggcaacccgc
    18241 caaaatcatt gatgaccaaa tgtgggtcga agatgatgta ggtttctctt gaacatttta
    18301 tgaacatgaa agatatggcg ttcagacaac agagaatctg attataccag gcactatccc
    18361 gacacccctg tagaaggatt gagaggttgg agcatagtct gtgtttgttg gattggagac
    18421 gatgacagca gcctttttcg ctgacggctg acagactgat tcaatgcgtc accgttccag
    18481 gactccgcag gtggagaggg cccgtacaaa cgggcctcca actccgaaac cgcagcagcc
    18541 aggtcaggag ccgaagtttc tgtgacaagc caggatacgg tcactgtccg gtcgaatgga
    18601 cagacgtgtt caagccaggt aagcaggcac cgcatggctt cccgagggtc atgctttcga
    18661 caggctcttc taaaagctga aaaatgaaca ctctccgact gccgatgccg gcgttgtttt
    18721 tcctgccacc aggcggtgag atgtgatccg tagcgaaagt tcaaccccaa gaggataacc
    18781 gccaccataa gagggatgat ggtgagaacc cacggcaccc actcacgacc gttcccacct
    18841 gacatatctt tctctaaccc cgaatcgggc ttgtcggaaa gagcaaactc ggccccatag
    18901 gcgggatcag ggtcaacatg gaacgaaata gaaggagcag tggaccgttg aattcgtagg
    18961 ttcgccacat cccaccagac cacttcaacc gcgggcaact cgtaatcgcc ttcctcttgc
    19021 atgacatagg tggcggcatc ggtccgttgg cccttgatac gttcgcctcg ctccccgcct
    19081 tcatccgtca cgacaggggg agagggatag acggccagac cgggaagcgc caagaactta
    19141 acggatggca gcaccaggga taacgaatca tatacggtgg tggtcactgt ccgggtgata
    19201 acgtccccaa ccttgaagag gcgatttgta ggttgcacga tttgggaaac cgtaagttct
    19261 ttggccccga taaagtaggg cagccctctg gcttccctgg gaatgccggc ttccagggtc
    19321 agtggaggag ttcgaaccac cgtggtagtc ttggcaagac caggctttat tctcacctcg
    19381 atcgagggta cctgataacg accggggact tgaggataga gggtatacga acgggtgata
    19441 ccgaaaaatt tttcaccgtt gacctcttca ttcaggttgg aggagcgtcc gggaggaatc
    19501 acgagggcgt tatctatatt gagactcggg aactgcggcg ccccggcaaa ccaggtggag
    19561 acaaatacct cgactaccag ctcgacaggc tgacccaccg tcaccagacc ttcggggtga
    19621 atacgggctc tggcaaaagg ctcttgcgcg gttgccggag gcacggtcat gatcaggatt
    19681 attggagtga tgacaagtgg gacccatgcc ctccaccagc ctcccaccct cctggtcctg
    19741 agtttaccca ttgcctcaac gttcacccgg ctgctccgct tgcaacgcaa atttggttcg
    19801 cagaaaatca gccggggtgg tgtgtaaccg gcgcagccac atttcggcaa tctgttcatc
    19861 gctcagttgc tccatctcca cctcgcccga ttgaccttct tttccttttt cgctgaattc
    19921 aatggcatcc ggatcgaagg ttggtccgcc agggtcatcc gacccgggtg gctcctccgg
    19981 agaggcagga ataagccccg cgactaatgc cttattctcc agagcctccg accaatccgg
    20041 ccggagtttt aaggccatgt cataggactg caccgcctga ggataatcgc cgattttcgc
    20101 gtaggcattg cccagattaa aatacccttc cggcgtagag aggcgtgcaa agcagtcaat
    20161 ggcggattca tactcaccgg cctgatagta ggtgatgcct ttccacatcg gattatcaaa
    20221 acgatctgcc gccgtcgcat aatcctgtcg ctccaaatag aaccggccct gctggtccgc
    20281 tgtcagccac agatctatga aggaaaattc gccgtcgttg gcggagcatc cgggaagcaa
    20341 aaagagcaaa aacatcggta tccatgggac tgtccagcct tttcgaaacc agaataatgc
    20401 aagcaatccc accggaatcg tgaaaaaata gccgaagtcc ttccagcgaa cctgctcatc
    20461 cttagccagc acagcttgta aatgactctg aacactgtct tgaatccgct ccacatcctt
    20521 gtcatcgata gtcgtgctga tgacctcgat ggcggcttgt ttgctcaatg cttcaagccc
    20581 ttcatagtca agtcgggcta tcaggcgacg gccattggcg tcgttaagaa accggttatc
    20641 gccaatgcga accggggcgc cttcccgtgt gccgacggcc agcaccatga cctgatcccg
    20701 ggtggattcc tgatgagttc tgaaaaccgg cagatgctcg agggcaattc catcggtcac
    20761 aaaaagaatc gttccggcaa cggtatcccg ttgcaacatg ccttcggcaa cctgcaaggc
    20821 gagagccgga tttttcccct gtacaggcat cacgtccgtc ctgaggtccg acacatacaa
    20881 atccaaaatc gacggatcat tggtaagagg caatacagag tgggcggatc cggcatagac
    20941 gagcagtccc gtacgggcat ttttgcgttg cgcgagaaga tcgcggattt tttgtttgac
    21001 ccgttcaagc cgtgtaggag gcacgtcaat ggcattcatc gtagatgaaa ggtcaagaac
    21061 aatgacgaga ggagcaagat cttcggtaaa cggcgtctgt tcccgttccc aggccggtcc
    21121 cgccatcccc agggatccta gggcaaccac caaagtgatc aggtgcaagg gctggaatct
    21181 ggagcctcca tcgccatgaa tttttaaatg cttcaacagg tggggctcaa taattccatg
    21241 ccattggcgg tcgacacttg tcctcaggcg aatcagcaga tagagaaaaa gggcggctcc
    21301 cagacccagt aaccagagcg gccggagaaa atgcagttcc gtcacctggt caagcatcgc
    21361 gccctcccct actccgcaaa gccagccata cggtcaagct ggcatgatag aaaagaagta
    21421 aaatcacagc ggctccaaaa gaccaaaaat acagcggatg tgtcgggcga taggagagtg
    21481 tctcaaaatt ttgcggctcc agtttatcca gttcacggta gacggcttcc agtgcttcac
    21541 gattttctgc catgaaggaa agccctttcg ttttctccgc gatggtctgt agtacagcct
    21601 catcaaatag gtcctccccg gccaggctcg ggtcaccgaa tcctatcgtg tgaatggtga
    21661 tgtccttggt tttggccagg tccgcagctt tttcaggtgg aactttactg ttggtatcta
    21721 ccccgtcggt tagaaggacc aacacccgtt cctcggcctc gctgttttcg aagacattaa
    21781 tcgccaagcc gatggcatct cccatcatcg tctgcggtcc gggcattccc acctcggcct
    21841 cggaaagaag cgtgcgaacc acttcatggt cgagggtaaa accaacctgc ataaacggag
    21901 cggtcccgaa aaacaacaac ccgatccggt ctcccgggcg ccgggcgata aaattatcaa
    21961 ggactccttt caccgcctca agacgctcta tctgccgtcc atccagatcc cgcatgtcgc
    22021 gggtctccat cgagcccgag agatcaacgg ccaacaaaag atcccggccg gactcgatcc
    22081 gttcgatcgg ctcctcaatc cacaccggtc gggccaaggc gatgatgatc agaatccaaa
    22141 agacaggcgc caggaatcct tgaagccaat tccgacgcaa aaccactgcc ccgcgtgtcg
    22201 gtctgccccc gatagcgccc gttaatagct cgaacctcgg aatgcgtacg gattcctgag
    22261 gttcatggta aggcggaaaa aacaaccaaa tgagtagagg ggcaggaagc agaaagaata
    22321 tccaaagata ttcgaattca agcatggtgc ctcctgatcc aataccggac cagacggaac
    22381 aagtctacaa ttttttcctg cttctcatga ggggagcttg ttgggacctc atacccggcg
    22441 tggataagaa gtctaccgac tccttgagcg aatccggtgc ctccgtagga ggtatccagg
    22501 aagcgaagcc atgcttcacc cgtgagggct gcgacctctt ctcgtgattt aaaggaaaga
    22561 gcggttcgtt tgaccaacac cggtagttcg gccaggccct tgacctgtga ctcaggatgg
    22621 agggcaagag cttcaatacg atcaagctcg attaaggccg attttcgata ccggctgagc
    22681 atccaacggc gccgaaaaca tacagcagtc cataccagcc ctacactgag cagaaaagcc
    22741 accaaatacc acccgggcgt ctgcgggacc catgcaacgg gctccggcat gacaatatca
    22801 tgcaacccag ggagggatgg gaagtcctgt cggccgtgta tctcgtccat agagttccat
    22861 tctaaagatg gttaccgcct cttgctcctt cgagcctgcg cctgacctaa caattcacga
    22921 atctggtcga ccggatcgcg atcagtcaac aggggcgcca ccggaatagc caattcctcc
    22981 caccattcca tgagagtctt catccggcgt tcgcacgttt ccagtaactt ctttcgtatg
    23041 gacccttttg cggtgtccac ctccacctgc aacgtgccgt cgcttaccac cagtcggccg
    23101 cgatccggca gttccctcga caaccggtcg aagacgggca cgacgatcag atcgttatga
    23161 cctgacagtt gagtgatcag ggggcgggtc tctccatcga ttccgtcgaa atcactgatg
    23221 atgataatca ggcaatcgtg cttgaccagc cggagaaggt tcttcagtac ccgattcaac
    23281 atggaggggt tggagcccac atcggaatca gcctggagcg agtggttcat ttccacaaga
    23341 gtgcggagaa tatgaaggac attccgacgg ctacgatgcg gcctgatttc caccagatcg
    23401 gaatcgttga acaggatgcc ccccacacga tcgcccatgc tgagaactct ccaggaggcg
    23461 agtgccgcga cctgcgcggc cactaccgat ttcatggccc gcttgctccc gaaaaacatt
    23521 tcaagtcgtt gatccactac cacgattgct ggccgatccc gctcttccgt atagacacga
    23581 acatggggtt ttcgtgttcg agccgtgacc ttccaatcaa tagtgcgggg atcgtctccg
    23641 gggaggtaac cgcggatttc ttcaaagtcc agaccacgtc cgcgcattcg tgaggcatgt
    23701 cgtccggaaa ggatgctgtg aacaggctga cgaggcagga aggaaacccc tcgggctttg
    23761 tattgaaggg caacgagttc gttaagctct acgtaaacgc cggtgtcgat catcgcgggt
    23821 caagatttcc atgtgagatt tgcctgcgtt ctactcaaca taccgggtta atacttccgg
    23881 ctcagatcat ccagaaatgg catgagatca tcataccttc atgatcccgg agtgatgtga
    23941 tcgtgaacga ctatgggaat gtgccgcgcc ggtaagctct tgcatttcca accttcacgg
    24001 ccggccagac gccagctctt caggctaccg ccaccacctt gatcaatttc tcgatcacct
    24061 gatccgctgt caccccggat gcattcgcct catagctcag cataatccgg tgccgcagca
    24121 catcgttgac cacggcgcgc acgttatccg gagtcacgtg gtcgcgcccg ttgagccagg
    24181 catgaacccg ggaacattta tcaagtccca agcccccgcg cggactcgcg ccgatctgaa
    24241 tcaactttcc catttcctcg ccatagcgat cggaatgccg ggtggcactg atcaggtcga
    24301 taatatactt ttcgatattt tcggaaacgt atactttatg aatttcatcc ctcgccgcga
    24361 agaccacctc ctgcgaaata ggggcagcct ctgctgagga cgccttcgcc tcatgaatag
    24421 ccggctgtgg ccgttcctct ctgcgtacca ggcggatcac ttcgatctcg gaggcttcgt
    24481 cgggatagtc gatatagacg tgcatgagaa aacgatccat ctgggcttca ggcagaggat
    24541 acgttccctc ctgttcgatc ggattttgag tggccatgac cataaacaga tcagggagct
    24601 tatgcgtttt cttcgcgacc gtgacctgcc gttcttccat ggcctccaga agcgcggctt
    24661 gcaccttggc cggagccctg ttgatttcgt cggccagaac aatgttggca aataaaggcc
    24721 ctggctcgaa atgaaactcc ccacgcccat ccacattatg gtagacctcg gtcccggtaa
    24781 tgtcggatgg aagcaaatcc ggagtgaatt gaatgcggga aaaatcagcg ttaagttggc
    24841 gggccagact tttgactgct cgggttttcg caaggcccgg gagaccttca accaacaggt
    24901 ttccattggc caggagcccg atcaggagcc gctcaaccac atgctcctgt ccgagaatgg
    24961 agcgcgacat cctggacttc aactcaagaa tggaatcgcg agcaatcata gtgtcctcaa
    25021 ttgtccaaaa accattggtg aacccgtaaa aaaacggccc cgaccaagac attctgaccg
    25081 gggccgttcc tgcaccagaa gaattattgg gatcctcccc cttccttaag tttttccatt
    25141 acctgatcca gactaaaact cgccgctttt tggcgtggtg gaaattcctt gaacgtggcc
    25201 aggaattttc cgacatactc ctgggctgga acaagcagga agacatggtc cagcaaccaa
    25261 tcgtaatagg tattggaggt aagttgtgcg cgctcatagg gatcacggcg caaattgaac
    25321 attaagggaa tccgtaagga ggtaaaggga ttggaccaaa gtttcagtgt tccttccgcc
    25381 cgttgctcca aaaagaccaa tttccaatcc ctgtagcgca aggcgctgag atctccatcg
    25441 tcggtaaaat aaaaaatttc gtcccggctg ccttttttct ccttccccgt gaggtatgga
    25501 agaaaattgt acccatccaa atgaaccttg aaggttttgt taccggcctg atggcccttc
    25561 agcagttttt ccttgatgtc ggaatctccg gcgattgccg caaatgtggg caaccaatcc
    25621 atatgatgca tgatttcatt cgagaccgac cctggttgga ttttaccggg ccaccggacc
    25681 atggcgggca cccgccagcc gccttcccaa tttgtgttct tttctccccg aaacggcgtc
    25741 atcgccgcat caggccaact gttcatatgt gggccattat ccgtcgagta aaaaacgatg
    25801 gtattgtcgg tgatgcccag atcatccagt ttttttaaaa acagcccgat gtgcctatca
    25861 tgctcgacca tgccgtcgct gtactcatcc tggccggaaa tccctctaag ttcaggtttc
    25921 acatgggtgc ggaaatgcat ccgcgtcccg ttccaccaga cgaaaaatgg ttttttcgct
    25981 tggtgttggc gatcaataaa atcaagcgcc gcagcggaag tctcgtcatc gacagtttcc
    26041 atccgtttct tcgtcaatgg accggtgtct tcaatttttc catcggcata ggaatgaatc
    26101 acaccacgag gcccgtatcg tttttgaaaa ttcggatagt ccttggcgtt tggatagtca
    26161 ggaagttcag gctcctcttc ggcattcagg tgatagagat tcccaaaaaa ttcgtcgaac
    26221 ccatgattgg agggtagatg ttcatcctta tctccgagat ggtttttgcc aaattgccct
    26281 gttgcgtaac ctaagggctt gagtacttcc gcaatcgtgg gatcttcccc tttgagccct
    26341 agctctgcgc cgggcaatcc gactttactg agaccggtac gatacacact ttggcccgtg
    26401 ataaatgagg accgtccagc cgtacaactt tgctcaccgt aataatccgt aaaaatcatc
    26461 ccttcatcgg caatccggtc gatattgggg gtccgatacc ccatcatgcc tttggtgtaa
    26521 gcgctgatat tggattgccc gatatcatcg ccccaaatga ccaggatatt gggcttgtcc
    26581 tgagcaaaag cgggcgacag tcccgcacat aacaccagcc cggccaaggc cagaagactg
    26641 tgacccgaga tattcgcgag gcgtttttga accataagca atttcctttc ttcaacaagg
    26701 agtaaaatgg aaaacttcac aatgttcatc aaaagattga aagctaattg aaggcaagag
    26761 caaccacacc gttccgtttg ttcctgaata gatcaagtga atattcgtcg caagtttgcc
    26821 cttgaatcgc agtccggctt ttgttctccg tttgaagaat accgtgcgac gttgatcaca
    26881 ggaatcacgt gagtgaattt ttccgcatat tcgtgccgca tattgacgtc cgctccttcg
    26941 gttttgagcg cttcaccaat caggtttttg gcgacgtcct caggcaccga tcctttttgt
    27001 ttgagggcaa tgaaacgcaa gcttgcgaaa acctgaagga acctctcgag tatccctgag
    27061 agcggaaaat cctaacaaaa tcctttcata aaagtaacca tgaaaaagca aaaagcccac
    27121 tacgattcac acatgtcaat atgtcatgtt gaacttcaag ttttatttta agggctcccg
    27181 actcactccc aacgtatacg aatgcagaga atgaagaccg aaaggaggaa aagcgtatgg
    27241 gaaccatact ggcaggacaa ctgatgacaa tgaggatgag cctgttattg gttggtttga
    27301 tgttgggaaa aaacgttttt gccaagccca atgttgccgc ggtaggacag ttcaaagaga
    27361 tgaatcgctc tgacctgatt tagttgaatc gggccatccg ggtcaggccg gaagaatcag
    27421 gcgatcgcca tcccgagtgc ctaagccatc tggaattata gccccatcat gaaacgccaa
    27481 ccagagaggg ctgtcaggag ttaatcccgc aacatcatca cagtcgatat tgacaatgtc
    27541 ggcgtaatcc gtgtcgatag cgagaaccgt gtcatccaag gcgataaggt ccccactcac
    27601 gcggtcaccg ttttcaagaa aaacgacatc agcctcactg attgccacat gtcccaataa
    27661 tgtccatgtg actaatgcca gacccaaggc tatgctggcg aaacgacctc gcacgtgaat
    27721 tcggtcaacc catagaacag ggaagaaacg gaatgaaagt ggccgggttg ccattgatgt
    27781 ggcaggcagt attctatttc aaataagtgg agccgggagc gggatgaatg ggaagaatga
    27841 ggcgggaatg tctcaagatg ctcctaaatg tccacactta cccaaggaag actattccgg
    27901 tcggtgcccc tcacagagct taatattgca ctgttgattt cttttcctct ttttccagaa
    27961 tcaggcgata tgcaatgaaa tatttgtaaa tattactcac gtattgaacg gtctcccgac
    28021 caatatgctt cgccgccacg atctccacat ttttaaacca ttgattcgga ttcaagccca
    28081 tcgacggcgc cttcgtccga agtttcgcga tcctggcagg tcccgcatta taggaggcta
    28141 cggcaaaaag ccattgattc aacaggctga ttttggggtc tttaaaatac cggtcatgaa
    28201 gaaaatgcaa atacttgact ccggcatgaa tattaggctc aagctgttta atattgggaa
    28261 tattgacgtt gggatcccga gcagtactgg gcaagagctg catcacacca attgcacccg
    28321 cagaacttcg ctttcgttgg tccaacatgg actcctgata gccaagggcc atggcaagca
    28381 cccaatcaaa cccatacggt cgggcacttt gtttaaatac atgcatgaga ttctgaaatt
    28441 ttttgcgttc ttgcgttgac acggcatttt caacgtaccg ggtgtttttt aaataccggg
    28501 tgaataacaa attcccgatt aaggtgcctt tcttattttt actcacaaac cggtttatca
    28561 cccttttcag ctttggactg tttttgcgaa aggcccaagc gatttccccg ccggcattaa
    28621 gagtaatgtc cggatgcaga cgaatatgct caaaaatttt cgcccaaaat tccgccttgg
    28681 ggctatccat gaccagcatt ggcaataaac cggcgttcac catttcgaga aggtcctcgt
    28741 cttcaagatt ctcctcttca gggatgagcg tgatctccgg cttcccggta gtggcgaacg
    28801 aggcattcaa gcgaacaagg tgttcgtaaa aactactgga cttccgcaca tggatggact
    28861 tgccggccag atcatccaga ctagagagcg gaggactgct aggacccgtg acgataatct
    28921 cgcgtacccc ggtgagaagc ggatcggaaa aatccacacg tttctttcgc tcaggtgtaa
    28981 tcgtaagagc accagcggca atatccccta ccccattgag taaatccgga atgagttcat
    29041 cacggttcac gggaatgaag atgaactgaa cctgcacgat cttccgctgc aaatcttcgt
    29101 taatctgctt ggcaaatatt ttcataaggt cgtatgtaag gccacgctgt ctgccgcggt
    29161 ctaaaaagta atatgttttg ctaaagggaa caagcacccg aatttgattg cggctgacca
    29221 tcccatccca atcgcccgtc cacggcttca gagcctgagg aaacttcggg ctgaacagtt
    29281 gattctccga ctcctgagaa gcccctaact cagagccagg aataaggatc aggatcgaca
    29341 gcgcaagaca agcgagccat gtgggctttt gaagttttgg aatcagcgtg gtcatatacc
    29401 taaaacctat catgtgcaag gtccgaagtg ccattatgac tatttttaat atatggaatc
    29461 caacacttga ttacagttaa aatcaaatgc aaccacaagc gtgtggtcgg atgaacctcc
    29521 actacaaact accgggattg atcacacgtc tgttttttgt tttagccaca ggctcaatct
    29581 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naactaccgg gattgatcac
    29701 acgtctgttt tttgttttag ccacaggctc aatctcacct gacattgccc tccaacggct
    29761 atcctatttt tgaccattgc ccatgttatt gaaaaaggct tctacacata aggacaattt
    29821 cacatatgcc cctttctgca atcagccagg cttctcaaga accaagaaag ctaacggaga
    29881 aacaaaaaat ttcattccgc cgttttccaa atcatcaaac catttgtcga aacaaaggag
    29941 agcattcatg cccaggagtg agcgattttt actggctctt gttttttttg ccatgtcggc
    30001 ttgtgcgaca actcaacagg caaacactgt caacagatcc gggtttctcg aggattattc
    30061 gatgcttcaa aaaggcgagg gagacagtga agcccttctg cgatacgtca acccagttgc
    30121 agattggaaa cactattcca aaatcatgat tgacccgatc caactctggc tgggagaggg
    30181 atcttctctt cgggacattc cccaagaaga ccgtattcgg ttgacctcgc tcctctcggg
    30241 caaactacag aatgcgctct tggcagatta ccggattgtt cgtaaggccg ggcctcgtgt
    30301 catgcgtttg agtgtggcac tcacagaagc cgaagcctca aatacggtgt tagataccct
    30361 ttccagtgtc ctcccaaccg ggtatgtcat ttctggcacc aaatctctca caaccgggac
    30421 cggaacattt gttgggagtg ccagcataga agctaaaatt tcggatgcgg aacttgggac
    30481 gcttttggcc gccgcggtcg atcgccgggg cggtgcaaaa tctttgtcag gagtcacttc
    30541 agaatggaat gacgtcgaag aatcatttga atactgggcc aatactctac gctatcgcct
    30601 gtgccaatgg cgcggcgaac acagttgtgt gccacctacg ccataggagt cgacaacggt
    30661 cgggagctcg tctctgagga gaaaaacccg gtaaggtgcg aatcgactcg catggccccg
    30721 aaggttcctc ctacttgggg tttcaatttg ccgggaatag gtggctgaat acaagacaga
    30781 tctttctcaa acgtccatgc tacactcccc aaaaatcaca cgattcatga ttctacccaa
    30841 ccctcccatt ggttattccc cacgatttat cacctgcatg attgttagtg ggctcaaagg
    30901 aatattcggt ggcctcgaaa aaaccatgta gaagctgggt catgatagga gtgatcctgg
    30961 gattcttcgg aagtcaatca acccatgcgg aggagattac aaaactagcc gaagaagtgc
    31021 agaaccccgt gtccgacctt gtgcgggtgg ggtttgtaaa tggtacgctt ttcggggccg
    31081 gggccaacaa ccatctcgtc aatgtattca atcttcaggc ctccaccaca agaagatttg
    31141 gcaactgggc cctcctcaat cgcctcacta ttcccatccc atatattccg gccaatgcca
    31201 tagaggataa aacgggcagt atgaccgggt taggagatat tgagtacacg ggattctttg
    31261 cccgtgacga atccaaacga cggtttaaac tgattggtgg tataggcccg acttttattt
    31321 tgaataccgc gactgataat catctgggcc tgggaaaatg gagcgtgggt cccacacttg
    31381 cgcttgtcag cataccggac ccatgggtag tgggggccgt catcagaaat gtctggtcat
    31441 ttgcgggaga gaaacaacgc ccgaaggtca acctgtttac acttcagcca tttgtgaatt
    31501 acaacttttc caatggttgg tacctgacct ctaccccggc aatcaccgca aactgggagg
    31561 cggaggacag gaggaaccgg tggacggtac cccttggagg cggattcgga aaagtggtgt
    31621 tccgtggaga aaaacgtccg atcaacatca aactccaggg attttattat ctggaaaaac
    31681 ctgaccgggc gcccgattgg acgctacagc tccaatttca aatcctgttt ccagataagc
    31741 ccgcttaacc ggcatccatc atttttccat ttttccattt tatcctcccg ctgtttcgga
    31801 acctccttgg ccgtataggg aggaagaaca tccccttgcg gccatggcat ggagcaaaag
    31861 tacaatcccc aacccaagag gactctgatg ttaatccgca cagagtgttc tacttatccg
    31921 tggaggatac tgggagtcca attggctgga ggggtcccta tcttgtccgg tgtcccagat
    31981 acggatacat ttcgccttaa catagagcag atcttttcgt ggaccaaaaa catttactaa
    32041 tgcttttttt gttccttcaa ccgtcccttc taaaagaaat atattccccc gagtggtgta
    32101 aaattctttt tcccgttttc caataaaacg aataagaaaa tgtctgctca tatcggattg
    32161 accgattttg ggtccttccg caagttctgt ttttggatag ggcagccatt cagctaatat
    32221 gagataccct tcgtctgttt gatcaatact caccactgca cctgcgagct tcttttcctc
    32281 gttcaggaaa cttgacgaat tgtcagctaa ttcatcgaac tgctggtcgg tggtcaccgt
    32341 ctttgtccca ttcgctttgg acgacaagag atcagagcct ggtaatgccg cgcatcccat
    32401 ctgcagactc acgagtccaa atattccaag tgcgatcatc cctgtcctca tgaacatcct
    32461 cctttgtgtt aagcaaaaag tcctgacaat gcagaaggaa acagcatgat attgattccc
    32521 cttgcatgga cgattttttt ctcatttgag agacctagcc tacctcgaac ttattgcgga
    32581 agggtcaagg gtttgttaca gaacggttaa ctttcacttg ttccttacat ccatatgcgg
    32641 ctatcaagcg gaacgaacgg gaccttccat ctcccgatgt ccaggatctc tcataaagaa
    32701 atctgctcga ccttattgcc ccaagcatcg gtgcagatcg aaggggggcc tgaaaagcgg
    32761 tgaagggaaa ctggtccgtt tgggtatgac tgccccctct cattcgtccc tgtcttaagc
    32821 caatttgccg aatggaaatg ccctttgcgc ggataagccg caggccccat gaagttgtca
    32881 cgggtgaagg aagccggaca cgcaagttcc gattcaaaca tacaccggtc agctagcatt
    32941 cctccgtgat cgatctgtgt cttttgcgga ataactgagg gaagcttagg tgcaccaagg
    33001 gctaggaacc tgtcggactt aggggatcga gagcgaaaaa gcggatgagc gaatccgaat
    33061 tttgacggtt ttgccggccc ataatgggac tatggaccag gaaagcggcg aaatatggat
    33121 tgcttagacg ctcttgcagc cggttcatcc taagtccgac aggcttatag gtggagttgc
    33181 acctggtatt ggatcatgat ggctatctgc ccattttcgg cctgatcacg gtggaaaggg
    33241 caaggtatat tcgactgact gtatgagcct ggggttttac cgctttggca ttggcccact
    33301 tacgttacaa acagatgaag cacggggcaa tgaaattttt ttgcggcaca atcccggtgg
    33361 tcagggacct ctgctttagc cgaccttata cccttttccc tccatgcagg tacgatgggc
    33421 acgatcaaac gtttcccggg tgcgctgatt ctgggctccg gcctgttgga cctgctgctg
    33481 atactcttgc tcagcacccc gctgtcccat catgcccagc ccgccgccgg cagcggcacc
    33541 gatggcggct cccatacctg gatcgcctgc aatggcccct ccgatagcac cgagcgcggc
    33601 accacccatc gcgccgaaca acgcaccccc gcctgatggc ggaggaggcg ctgcagccag
    33661 attcggatgt tccggatcgt aacccgtttg ctgcttagcc catttatagc aagtaaaatc
    33721 gtcctgttcc tgctgttccg gactctgtcc cttttccgga aatacaaagg tccgcgcctg
    33781 aggaggaggc gcggagtcga cagaagaacc cgatggctgt cgtccaccct gctgagtgcc
    33841 cacacagcct gccagcatga ccacgcatga caggataata cacgaccgct gtaccctcat
    33901 cgttttctcc tttattcggt caaggaccaa tgccctaacc aatcttgtag cctttggctt
    33961 ccatacacac gttatgcgca cggtcaaagg tggcccgggt ctgctggttc ttagccgagg
    34021 tttgttggac ctcttcctga taccctcgct caacccgccg ttgtcgcatc atgcccattc
    34081 cgccgccggc agcggcaccg atcgccgcac ctttacctgc atcgccagcg atggcgccgc
    34141 caatggctcc gagtgcggca ccacccatcg ccccacgcat cgcaccaccg cccgatggcg
    34201 gcggcggcgt tgcggccagg tccggatgtt ccggatcgta gcccgtctgc tgcttggccc
    34261 atttaaaaca agtaaagtcg tccaattcct gctgttccgg actctgtccc ttttcaggga
    34321 acacaaaggt ctgcgccgac aagtgggaca catggagaag catcccgaat ccaagcgcca
    34381 gccccaagac catatacgta cgcatcaatg cctccacatc tagagaatga ttcgttcagc
    34441 acttacttct ggtcaggaat atgataggtc aggttaggcc acaccgcgac tccctctccg
    34501 agttgttggg ccgactcaac cccgctgacc atcgtaattt ctcgcgcctg gatcttcgaa
    34561 atcatttcgc cctcacccgt aattccggca aatttgccgg tgccgccggt gatcttaaat
    34621 atcccccggc acccttccac atcaccggtg caattgaact ccgcaaagac acgattgccc
    34681 tgttctcctg tgataacgca tttggcataa cccgttttcg ttcccttttt catatcggca
    34741 tctacggtgc ctggacagac gatcgacgcc gcatggagcg ctcccttggc gtcatccatc
    34801 aacatcacgc cactgtaaac ggccaccata tacacttgat caggtccaac cggaaaggca
    34861 aagccgaccc ccttccacgg ggacatcgct ttaacgctgc cttcttctgc ataaccattc
    34921 acgctcagca ccaacgtgac cgcaatgaga actgtcattg ttatgattcc gctcaccaat
    34981 cgcttcatag attctttctc ctttgtataa gctaaaatgt aacgatgcag gccaatatag
    35041 caaatttttt tacgagggca aaagctgcat ctcatcccgg accacaaatt cctttatttc
    35101 catacagcac attcgagcag cgacttcgtt ctttgggaaa agcaaagcgc gttcggtatc
    35161 tttcagaatc taatggcttg tcatgaataa tgatagccgg ttcccatgcc acgcgtgacc
    35221 aagcctgatc cgcactttcg tctaaaaaat ccgatcaaaa agcccgtgct ctaaccggca
    35281 cttcaactcg tcatcacttc aatagccgaa cggcagccaa tttgtgttcg ccacgatatt
    35341 ttccgccccc agactcacaa aaattgtgta agggaaaaag ctcagaccaa aaagcctgtg
    35401 gcatgttctc gatttttagg actgcttaac ctacaatgcc ccactgataa gcttcacaga
    35461 tttttcctga tccgttttga actgtcacta ctaccgattg aaaagcattc ttgctaatcc
    35521 aataccctaa cggaaaatcc tgcac
//
LOCUS       DAORAQ010000015        35207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_259_length_35207_cov_69.528562, whole genome shotgun sequence.
ACCESSION   DAORAQ010000015 DAORAQ010000000
VERSION     DAORAQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 35207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35207
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_35207_cov_69.528562"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..372
                     /locus_tag="PKM72_02805"
     CDS             70..372
                     /locus_tag="PKM72_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59740.1"
                     /translation="MKTQHQAIGFFLAVILAILPLSSALGVQHILGGADLAGHAHSDF
                     DLCDWVKIQTTGSLSLDANSLWYGLSRSGDAVVQFSSRFNNQSVISSSAPRAPPQL"
     gene            933..3071
                     /locus_tag="PKM72_02810"
     CDS             933..3071
                     /locus_tag="PKM72_02810"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNP59741.1"
                     /translation="MSWNDAMLTEVSDFSVSFIAHVSGYAIGMILYAMLLWLVFQALQ
                     ESTQKGQSEPSAQSPRWLACWTAILGLLWNVGALLAFLLPNLHLTAPLPVLYALAVSA
                     LGFLPAVVVLAMVKTQPIGLPAWIRRSISASGFVLSGWASLMHLHAALSGESGSMSGA
                     ALQAMVWGFLVLLIWLLAVRVRAEGWDYMGGIVVLSGLAIAVLPFSQHETGEYSWGME
                     VVGHHANLLLALAILYQDYRFALGDLFLKRALSFLVLALVTLGALITVGIPVLRLDPD
                     NEMANLSRLGVFLALWIITALAYPHLRKAVIWLVDVVVLRRPDYDQLRQEVAIEVNQE
                     EEPERILSHVGALLQPALLARQITWRPWRLDGEAGSEWPGQVGSFDGDREEESLASGA
                     VSVGSLVRKGSRGSSLATPCGEEFLVVIPTMELPQYVLVIQGFFHRRRLLSDDLVLLE
                     AVAHIIARRIDAVRSAHERCAVAIREQEMRKLTIEAELRALRTQLNPHFLFNALTTIG
                     YLIQTAPARALDTLMRLTELLRGVLKHLEGDFSTLGQEVDLVQAYLMIEQARFERRLA
                     YQVNVPSNLRDLRVPALVLQPLVENAVKHGIQKCAKGGVIKIEAEVEQRKNENDHGAW
                     ITPELVITVQDTGHGLSGKGQEAAWGAGIGLANIRKRLQLHYGDHATLSMHRNPKLGT
                     IVHIRIPLVRSSDLLAEESECLHSVQGAKE"
     gene            3068..3847
                     /locus_tag="PKM72_02815"
     CDS             3068..3847
                     /locus_tag="PKM72_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNP59742.1"
                     /translation="MSGKIRVIVVDDERPARSFLATTLKTLDDVDIVGEAENGEDAIS
                     LIQREQPDLALLDLQMPGMDGLSMVKAIKKGHIPLIAFVTAHNEYAIEAFDIEAIDYL
                     LKPVDRLRLRKTIKRAKERLERDDVMANELEARPRGQEGGPPEDSDYMDRLPIKKKEE
                     IIFLPVNQIASIVSDGELMHITTLRKERHTISHRLKALEARLDPRRFVRLGRGTLVNV
                     ESICRVATMPGGSYVVTLTNTQQLRVSRSQSRFLRHQILRL"
     gene            4166..>4504
                     /locus_tag="PKM72_02820"
     CDS             4166..>4504
                     /locus_tag="PKM72_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59743.1"
                     /translation="MFKKLRSQAGAALVEYSLLVAGIALIGAAAVAVFGHKTSDMMAA
                     VAAVLPGAHADDNAPIVAGKIIETSVDGGPGGDAIALDIGGIVANAGTERLGNSFGTT
                     TSISTLVVEAQ"
     assembly_gap    4505..4604
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4947..7025
                     /locus_tag="PKM72_02825"
     CDS             4947..7025
                     /locus_tag="PKM72_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HNP59744.1"
                     /translation="MKDNPAGLPKPVTLGYQNIRRMSLLVSGIFLLLLPTAIAQTPSS
                     KLSPRTIVLKKSPESPTPLPVIVAQHRRVLIDDPVQRVAVGDTSIVSAQLINNKEILF
                     LGQRPGRTSVIVWLQSGAIRHYQCNVQLDLSVLHAALHNVHPSIGVTSAPDRDALILT
                     GKVPDVTYSQAAEGIAQKYVNARTGERKAGQGPFVKSSDGQRANQSQPGRSSQSSGSS
                     SSSSDDEGDGESPSSDSSAHIEVAEAIPVGGTIINLIQLDTLPATPEERMQEAIRNLG
                     GEQVTVRRVKRGIVRDDEQDVFVLEGSVKNQVALLRVLEVGSRMLTGHGATRHSIRVV
                     GDEAGALAGRLGQQQGGQQGQQGRMMIGGGGGGIGQLFGGAGRSGRVSNLVQSNLGRA
                     KVIEAANGRIISFLHVRDLPQIRVNIKLYEVNRTKLRSYGSDFVGTFDNFGSAPESGA
                     RNILGFLTGTLVADTFIRSGEFAIRSVFTYLESLDLARSLASPSLTVLSGEIAAFLVG
                     GEVPIEQAFSPFFGGGDITGAAGVFQFVTFREFGISLRVRALVGEDGDLTIDAVPQVV
                     TPDLTLTAGIEEATGTNQPTTAFKNRSMQTSARMQDGQAILIGGLLSRDKSDQQDFIP
                     GVRDIPGLGWLFRRFTQNDEEVELVIVIHPVIMREPIPEVGLWAFPALTDYRPAWEFK
                     SWPAPEEKGL"
     gene            7022..7951
                     /locus_tag="PKM72_02830"
     CDS             7022..7951
                     /locus_tag="PKM72_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNP59745.1"
                     /translation="MKWSVKALMTKAHPSFGPVASQRGGVLIEFAFLSLVFYVLVALV
                     VDIGRMVFAAQVLQDAARVAARELALVPLPAAMTFEQALEDPTVLAQVFDPALLVIDL
                     DTFGDDDVAFQAFLDQLPMVNKMLRPLMIFEQADIQGSGRRLLRFPGALLNDPSAPSG
                     LGVGIPRVESRGADGVEAIRWVPVLEEITNGSFSLVPLDPENPPPQRGLVAVRINYPF
                     QAAMLSGFQQGPGGPFEPNLDNRIQADDESVDDSINAPSRGTPFGVGDLPEVGVYGGR
                     FGLGGQLAFGGETLRPYRKLLSAQAISRREVFL"
     gene            7948..8973
                     /locus_tag="PKM72_02835"
     CDS             7948..8973
                     /locus_tag="PKM72_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59746.1"
                     /translation="MKTAGFHPRSPWVRFVSSPFFLAGMLVVILGGLWVSGLVNPCWF
                     GVNCGPSTEGLVAVPVSAQTIPAFTQLNRQHVWNAGKGQPAVVLLPPEQVTEAMKTNL
                     TDILGRVLGREKPAGYVFTEVDFLPRGSRPGLVGGIPPGKRAVRVEAGQVSGLYGLNP
                     GDRFDLIATLPIDADGKDNSLTMGGVYGQQLALQANLHNWAKQATVRVLVQNGVLVQP
                     LTTREIPLSMNTLTQGLVVKKKPVQEIVIAVDPEEVASLTEAVAVKAEITTIPRSGHP
                     DDPGDSETPESQPWSPFGNTLALGKSSGGISKNGNTESSLTFVETLNGSGERELITVP
                     VASDQEK"
     gene            9041..10270
                     /locus_tag="PKM72_02840"
     CDS             9041..10270
                     /locus_tag="PKM72_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNP59747.1"
                     /translation="MLVLFVLLVWGMLGIGSLVIDWGYVNLTRVQMQNVADAAAGEGL
                     RLRDVAPEDPVASDLNRRVAVSQLAAWTYDDDFDLTDDLLQFGAGPDVFLTGGHTDLN
                     AMQVLDVQPAPVYKPVLQWNDEANARHGDMVSGTFGISESGCSVKAPHRECANYTRID
                     FVPAEVNASPTADAFLVRLRRSIKPEPFQGQAGVDNVPGVSSAGPTLPLVLGLGSPLQ
                     AQIDSPGIRFTGITVRATAIADARPVRGVGLPDSLSEPPRLGVAPFALARAGWRDSLV
                     MDVPSSGTVDSSGVVAVGSTAIGRFLLPVTSIGEPVLARFPEGGQVTIDGYAPLYEDF
                     LGTERVVGFARVEMTGTSPGSVQIIRRPGQVAVENATVTLLPAFPLLPEPELALLLQV
                     NRDIQKEGALLAPALVR"
     gene            10285..11505
                     /locus_tag="PKM72_02845"
     CDS             10285..11505
                     /locus_tag="PKM72_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP59748.1"
                     /translation="METNLHILVVGKSPGLRAEFDSALQGVGNYRCVTVYAESFRHAE
                     ELARNRRPQLICVEMNGDLRGLKEFTIDVSQMLPGTAIAGLYKPDLFESDQSESAFIL
                     EGVRARIQDFLRHPLSSTEIRQVLDRVFVQGKTKSSLSSLGHIVSFVSNKGGVGKSTI
                     SVNMACLLASRHPGQVLLVDASLQLGVAAVMLDLNPRTTIVDAVREKGRLDETLLREL
                     TVSHPCGLSLLAAPGNAAEASEVDEESMYRILNLARRTFMYVVVDTFPMLDSVVMTVL
                     DLSTFTYVVMQGTAPNVIGTAKLLPILEGLGCPVERQRLVLNQNYRNFAGNLTQTEIE
                     ERLMREFHYAFPYQKKLLTCANLGEPYILQTTRYFGFGRSAHLLVGEIEQLADRAVAA
                     EIDHLAKLMNEEPA"
     gene            11519..12973
                     /locus_tag="PKM72_02850"
     CDS             11519..12973
                     /locus_tag="PKM72_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNP59749.1"
                     /translation="MSEQDFPFPSSSESRPPVPFGKSLSADTASRLKAALKRPAMNVV
                     KDEALLDDPLARVKHADYLSIKSALQERLLNEIGEHLAAGLNHEDVSAVVKEFTARIL
                     ESEHIPLNQAEQGRLAEELTEEAIGLGPLTPLLADPAVTDILVNGHQSIYVERYGRLE
                     KTDVQFLDDDHVIRVIERIAAQMGRRVDTASPMVDLRLADGTRVNATLPPATIDGPTL
                     SIRRFGVRRLRRNDLLGLGTLSPDMATFLELVVRGKKNLLISGGTGAGKSTLLGALSE
                     AIPFHERIITIEDTAELHLDQEHVVRMETRQPNIEGKGRILARDLVVNALRMRPDRII
                     VGEVRGPESLDMLQAMNTGHEGSMSTVHANSPRDALSRLETMVLFAGTELPSRAIREQ
                     MVSALHLIIQVRRYEDGVRRIETIAEMTGMEGLTPLMQDIFQFRRRGFRGKRVAGEFV
                     PTGIVPRVVEELREQHIDIPLSLFTGGRDLHGFS"
     gene            12960..13823
                     /locus_tag="PKM72_02855"
     CDS             12960..13823
                     /locus_tag="PKM72_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNP59750.1"
                     /translation="MGFREFAGLMAIGLIVGLFVWWWRNFSFRVAIQARLAQDGAGRE
                     SPQTGRSVRRVFPPRYRIIPACLGVGASVGLFYFVEFSPPFAGAAGLMLGVLGFLLES
                     IIAGKRLSRIETQLADSIDLMVGALQAGLSLPKALDTARQESVAPLRPYLDNLVRKIR
                     LGENPATAVRELGERVPLETFQLFSLTLSAQWWTGGSLAPTLSVVGRTIRDRIELSRR
                     IRTQAVEAKASVIGVLAISYVLTFIMWRANPRPLENFFNSDIGSLLAGAAIGLQALGI
                     VWINRISQVKF"
     gene            13835..14797
                     /locus_tag="PKM72_02860"
     CDS             13835..14797
                     /locus_tag="PKM72_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNP59751.1"
                     /translation="MDRMWYFGLIIVAIVASVMLTPWAGMFVALLLLLFGMVSWASRH
                     RSVERVRDTLESERSVLDLGRARSEWWLRRWLFLSGFRGRLAPVVFMGLTGGALALGI
                     ASAYIMNRSGVVDVMVVPLQGIPGGIGEAFISVAYAGPWIVLSLLASIPLLVVRASRR
                     QRVMDVDHDLPLMLELLATLTEGGLSFDAALDKILGAQFRTRPLSQEFRTYHRELQLG
                     VSRLQGLRRLAYRLEVTSVSFLISALIQSEQVGASIATTLRRQADDLRNRRREQALML
                     AQALPVKLVVPLILCFLPGIFVSTLGPSLHQLVNLVSGMTGGIR"
     gene            14838..15605
                     /locus_tag="PKM72_02865"
     CDS             14838..15605
                     /locus_tag="PKM72_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59752.1"
                     /translation="MKLWAAYGLIVGLLVCSACADAPYLRNQYETACTLPDLQLAEAW
                     DLLEEARSTSGGCDRDARGMDRCRALRAEIERIAFVCPNHIPSLMTTAVLAYEDRDLG
                     KAGERLTSILDLVKVHPDAAVLRARIALEEGNLPFALTFLKQRMELSPDHAQLREVYA
                     ATLFLSGRLEEARTQLAVAEQLGAPRWRVAYHLGLFEERKGELQKARLLYQESADLKA
                     DWNLPISRLHGLLAKPLSDKKPSALDGGYFELPSLLK"
     gene            complement(15830..18532)
                     /locus_tag="PKM72_02870"
     CDS             complement(15830..18532)
                     /locus_tag="PKM72_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNP59753.1"
                     /translation="MQAPLIDPTHLPDTIPEAFFALSAIAADHVVMQIKEGEVYRQQT
                     YGEVSKLVRGLASGLHEHGLHPGHRVAIVAENCPEWVIAHLGILTVGATAVPLDIQMP
                     QEQLLSFLTTSNSRFVFVSSKTVDLIRDLPATITVVNMDSLTESHHLSMKDLMDQGQE
                     KPSVDLRVNPDDVASLLYTSGTTKKPKGVLLTHRNFMANAKDIIGKDLAGPEDNFLVM
                     LPLHHAYPFMIAYLVPILLGARMTFLSSLKGPDLVQCIHETGITIAVGVPQIFTMIRR
                     SIFEELDRRPGLVRWIIRLLLSLSDFVRTHTRWNPGRRFFAPVHRRFGPSLHLLCSGG
                     AKLDPQISKDLGNLGFTVREGYGLTETAPVVAFSSLSRLKPGSVGPPLASVEVRIDQP
                     NESGIGEVVVRGPNVMKGYDQAPAETAEAIRDGWFHTGDLGYLDSDGYLFLTGRIKEL
                     IVTPGGKNILPEELEEAYKQNPAITELCILGLPRAGGEGEHLHAVVVPNFDYLREHKI
                     HDSATYIKDALNSVAATLPPYKRISGVTFIKDPLPRTRLGKIQRHLVLAMTQTKQTAV
                     EPAPEQEPETDQEIRETTIGQAVIQTLNRLISADRALRLDDHLDLDLGFDSLKRVEFQ
                     AALESRLGPVPETFMGEIVTVRDVITKLMALQQIPAAQTETTSSWHQIFETPLPPALT
                     NTVLAPLSRGNKIIGQIGMAIVGSFSRIVFPLTVKGLTHLPKEGPFILAANHLSFIDP
                     FIILGTVPPSTFSQLYTLGWEPFFRSPFRRWVARVGHVIPVGPETPMVTVLKASVALL
                     RKGKSLLIFPEGERSLDGRLLPFKKGLGVLACELHIPIIPVKIDGSFEVWPPDAKTPK
                     RHPITLTFGQSLCITPSMVETWTTRGEDPHLRATTQIRDAVASL"
     gene            18835..20217
                     /locus_tag="PKM72_02875"
     CDS             18835..20217
                     /locus_tag="PKM72_02875"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNP59754.1"
                     /translation="MPFFKVEDTRDLCVGTFVKLEGSWFSHPFPTNTFQIKSSDDLAT
                     IQALKNVVILQDPDRSALLDTEEDSDQNRRPLNFEESASREEDTEAIFDSELDPPADS
                     GPLIKESSLSTILDRREDYHEFQDHLRKVEGAYQKTLGQGNELFRQLSERRPSGRKTA
                     DAMITSIVTAIQHPHTAMSLIDVVGSNGATWGLSEHALNVCTLSLLIGRQLGLEAEGL
                     LDLGRGALFHDVGYRALPMNVRFSAGGMKIESDPELGKRHTEVGRQLMAAFPDTSPAV
                     LEMIGHHHERLDGSGFPKGMRASGLSLPTKIVMVADHYDELCNAPDLQTSLTPHAALS
                     RLFRHVVMKEELSRFCPEVVQALVQAIGVYPPGSLVELTDGFLGVVARINIHHPTRPT
                     VLLYAPWICRSDGMLVNLAQEPELEIKQALHVKDVPLNALVYLSPRRMAMFVHATEPT
                     TMAFNSRRRR"
     gene            complement(20399..20761)
                     /locus_tag="PKM72_02880"
     CDS             complement(20399..20761)
                     /locus_tag="PKM72_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59755.1"
                     /translation="MDQKVFPDPSRSTRRVRVFISYPHKNQDPYLERMKVCLKILKRS
                     FSPRVRARPAIVSRRALGGENPPTIRTGRSGLFVDWSGFHRLGLLFLQGNGKGAFQGK
                     NPSGENRRMKFRLALFGA"
     gene            complement(20763..21038)
                     /locus_tag="PKM72_02885"
     CDS             complement(20763..21038)
                     /locus_tag="PKM72_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNP59756.1"
                     /translation="MEQLTTLHCSNNRLTDPAPLATLKELTILGCAGNQLTDLALLAP
                     LSLDGKDFVALAVLEQARQNGKAAGDFPCTRHCGGATLSWVPPVTGA"
     gene            complement(21068..21331)
                     /locus_tag="PKM72_02890"
     CDS             complement(21068..21331)
                     /locus_tag="PKM72_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59757.1"
                     /translation="MNPDQIERDYQQALRRIREAKDTLAASLDLSCLIWEGLPPELFE
                     LTWLEELALQQSVESWKGPKGTIPLLPQEFAQLPGLRHGILVR"
     gene            complement(21434..22876)
                     /locus_tag="PKM72_02895"
     CDS             complement(21434..22876)
                     /locus_tag="PKM72_02895"
                     /inference="COORDINATES: protein motif:HMM:NF023407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HNP59758.1"
                     /translation="MTAPSSITLSLISHTNIGKTTLARTLLRRDIGQVLDQAHVTLQN
                     EHFVLLETSDGSRLNLWDTPGFGNSHKLLGRLQGLTNPIGWMVSQVWDRIADKPFWCS
                     QQAIRNVRDEADVVLYLVNAAEDPSMAGYLQPELDLLTWLDKPVIVLVNQTGLIDPQE
                     QRQLESLWRQHWVNHRVIKDVMSLDAFTRCWVQEGVLWDHVTQALPSEKHHTMKKLEK
                     AWYATHRQIFDASMTRLAQLLAETALDGERLSQEPTGLSKKPQIKNAIHALDQRLAQR
                     ISAVTADLIELHGLTGNVPHTLKSRIEDVIVPGERKPWEEETFWGALASGAAAGLASD
                     LATGGLSHGAFTIGGAILGALAERTYAKSQETEDSNRISWVPAFLDRQTRDALLRYLA
                     VTHCGRGRGDYTDPREFPLFWQRATEKALEQRKDDLHQLWKLTQSPQPLTGITDHLQT
                     NLAPLLSEMSQEILGQFYPEAKGWLKQQAR"
     gene            complement(22873..24363)
                     /locus_tag="PKM72_02900"
     CDS             complement(22873..24363)
                     /locus_tag="PKM72_02900"
                     /inference="COORDINATES: protein motif:HMM:NF022518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HNP59759.1"
                     /translation="MNSRAASKILLIQALEQSDPQARYISHATRQRATQQAKAHSSHQ
                     ANPSTESSIQFLINRAESLWNFLSTSYPIITESFRGVQVTIPFTFVAIPALAAGLLIN
                     GLGTSQQVNLLNFPLLFLLLWNIGTYTGNILLPLLGNDPNGPLLRHLSKGFATTTKWW
                     RKGPWPKIALPERAGREWIPQSTERFMNLWWKHCHPIIISRVRHLLHIGSACLALGII
                     LSMYVRGLVLDYQATWESTFLSATQVHTVLHLLLGPAAWLLGFPFPSAVDLADLQAPG
                     HGSAAPWMHMWALTAFVGIVIPRVILAWLSARFAHKAAESIPLNLNEPYYLQLLSTER
                     GQGIQIDILPYSYQPSPATLDFLGQCLLDLLGNQATLHWRDPLPYGQTSVPWLQTNSS
                     PQTMVLVCNLAQTPEAEVHGELFHMLQTSIEPCNGQHHLLLVLDQEPYRHLADQAQMT
                     GRQQTWQRLANDHHLQIVMFDAKNTLRDQLLEKAQAALWPPKGEMT"
     gene            complement(25138..26001)
                     /locus_tag="PKM72_02905"
     CDS             complement(25138..26001)
                     /locus_tag="PKM72_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNP59760.1"
                     /translation="MRLSIWVILCLTVGLSGCAGNAKSTKTQGGMESEPTIQMVEFED
                     GLHSEGEEKETEFFDPFDESDQAQGKDYDPLEPVNSAVFEFNYRLDKYVVKPAAKVYN
                     FFIPPDVQQSFSNVFQNIRFVPRLFNNLFQAKFQGAGIELSRFLINSTLGVGGLFDPA
                     KIMFDLDTPQEDMGQTLGSYGVGPGPFLMIPFLGPFTLRDGIGFIGDSFLDPFNWLVL
                     PFIEIADAPRLVQHDPTVTFSRLGMVAGETVNLRALTLEKFQGVEEGTLDLYGAVRNA
                     YLQQRRKAIQE"
     gene            26593..28215
                     /locus_tag="PKM72_02910"
     CDS             26593..28215
                     /locus_tag="PKM72_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNP59761.1"
                     /translation="MSPSVSEQKASWNGSIKRGKKQDRHPVSSGGGSPDVDRSSGISD
                     KQLPLYLKEIGQVSLLDRDGEVRLCKKIEEARRSMLDILYSLPMTLSYLQEQRMRLMN
                     GEIVAKHIVQKEKEEYVEAESEEECDIPDIQAEQLEDEEFRQRVIQQLSHLCHCVEFM
                     MTKDNDSTAMPGASTSVQARKKLWQSLEGVNWHPAFTKQVECRIRAIERQLAELLKRL
                     GISSREINNSGEATKGMSRESFSVRNTVIEATSSSHRMAREAGEHLVYLEQEVLRMGF
                     SEFLARVGQLDQAKIRLHFAKEAMLEANLRLVVSVAKRYVNRGLDLLDLIQEGNIGLM
                     RAVDRFEYQRGYKFSTYATWWIRQAVTRALADQSRTVRIPVHVCDVLTRVRRTLERLA
                     VQLGREPKLEELSGAVDIPLDKLSTMLEATKGTLSLETPMGDEDGSPLSDLLQDHAAV
                     SPCYSAERMDLQEKVTTLLGTLTPREAHIIRRRFGIGELEDATLEEIGIEFSVTRERI
                     RQIEERALTKLREPQRNVVLRNFFQPSGPALE"
     gene            complement(28294..28968)
                     /locus_tag="PKM72_02915"
     CDS             complement(28294..28968)
                     /locus_tag="PKM72_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNP59762.1"
                     /translation="MASIEDRPLQPPPNEVEDDRALLLHTHQNWRRSQQAGIKKDKQS
                     EHEQSTHTAPPSSQDFFQIDLREADFRHQDLRNANFQEAYLPGANFEGANLRGANFQG
                     ANLRSVRFRGAQLAWANFSQADLIWADFQHANLQGTEFQHATLRGATFVRAQIRDGNF
                     FCSDCAWADFQDTDLYGSDFQQANLNGTTFLNAQTAGANFKGSLGHHESSTTESSPPS
                     GDSLFP"
     gene            29220..29924
                     /locus_tag="PKM72_02920"
     CDS             29220..29924
                     /locus_tag="PKM72_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59763.1"
                     /translation="MELTIFVLWTVVALLGYQLWVLWKAHGSFLQILKELPDRIRSGL
                     GVVGVRELKDIQERFYRLEQRDHIRKIPETTGEKIDQHFQECFQKVDPPVDCLEPIDA
                     SLFAMDGPVPRSRSIMWKGLRFTLSDSIWFHAGVVSNEDVEDDQVQSMVHGPFCQRCL
                     KRLVGRIRGQDSGVPPQCHNCGLSWRIHEPDYTEMPLGDLKRMVYAMLDRKVRISRNV
                     QCEVFIVGEEDSGAIQ"
     gene            29983..30684
                     /locus_tag="PKM72_02925"
     CDS             29983..30684
                     /locus_tag="PKM72_02925"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HNP59764.1"
                     /translation="MLLCPSCSQENPDTANFCLQCGKALGSPPSETVPEVNSEEPGAR
                     TPPTQSQLWETFIGPSKSIQFSVKTGWVWRSAFLYYKEKFENIETPQGPRFALSWNWP
                     AALFDSFLWFLYRKMYLFALLYAVAPALAIFMTGDVMVGIVSRILAGASANYLYYWHV
                     KDKVQRIMAMPGLDDTGRAHLIQEEGGVQPYVLWLGVVLHMLMFGLLAATLVQGPPVE
                     DPLGGTEGRPVQKFF"
     gene            complement(30681..31571)
                     /locus_tag="PKM72_02930"
     CDS             complement(30681..31571)
                     /locus_tag="PKM72_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59765.1"
                     /translation="MTRKFFPCFRYDMVSDEHKLEQFARWWTALAGRHGVADRTEQFF
                     RQGLGPQNALDRAAQEGGDLDYILFTLIRHWIPAVLPPAGQERTAKNDSDYWLESERI
                     LKTAVGRLRELKPVIDMLTTPSPLAMPADSPRSEVEVELANMLEGIAELVGEYGGPDY
                     TSIIKNFDPIPLRQTQPFKHNKKHSAELWVVFLLREHFRNLGLGKDRAWKLITQIVDA
                     ADIFQSDDQPSSPEALKSWWTKNWPRTYTLLTEKGKQGDAKSTAYQSDYDWFRAWFNW
                     QTASNQSQGHPAIKNPDSPS"
     gene            31665..32270
                     /locus_tag="PKM72_02935"
     CDS             31665..32270
                     /locus_tag="PKM72_02935"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNP59766.1"
                     /translation="MAQLVLIRHGESQWNLENRFTGWVDVPLTPKGEQEARDAGKKLK
                     SFQFDCAFTSMLMRAKETLRLVLEEIGQTTIPIEENKALNERMYGELQGLNKTETAQK
                     YGDQQVKIWRRSFDVPPPGGESLKDTAERVLPYYENRIRPALLADKTVLIVAHGNSLR
                     SLVMQLEQLSKEAVLELNIPTGAPLWYELATSGEVTDHRYL"
     gene            32362..32751
                     /locus_tag="PKM72_02940"
     CDS             32362..32751
                     /locus_tag="PKM72_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59767.1"
                     /translation="MVVIFLFFIAGLWMADGVALLIAPERMIATLRQSLIVAPGFIKW
                     GGVAALLGILLLFGTRGIPYQPLWIVVGLSMVAKGIFLYAGSDRWRLRIVKWCLEREL
                     IDYRIWGLALCTLSVLLLDALGWGKGP"
     gene            32816..33109
                     /locus_tag="PKM72_02945"
     CDS             32816..33109
                     /locus_tag="PKM72_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59768.1"
                     /translation="MKDIDREKLYDTWQIHQSMDWPDGLGGDEGQLMTLDTVIGGCLT
                     YYLDEHHLDEARIEILRDCLAELESLVPDLTEATSEYFRRLQFLGVTLLQDFS"
     gene            33299..34219
                     /locus_tag="PKM72_02950"
     CDS             33299..34219
                     /locus_tag="PKM72_02950"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNP59769.1"
                     /translation="MSQQNDLKTILNKLHYSDDAKVVAQISAQTKLVMARMKGIRRKV
                     VVMSGKGGVGKSMTTVNLALAFARMGQRVGLLDVDINGPCVPQMLGMRGKGLLDTPEG
                     AVPPTGPLNIKVASMDFLLQENAPVRWKGPMDLSPVWLGMTEMNVIREFLSDMVWGEL
                     DYLLADLPPGAAADKPPLLAGLIPDLAGAVVVTTPSEVASNVVQKSIAYAQELGIEIL
                     GVVENMSQYRCPSCGEESPLFEGDTDSMCEALGLPLLGRIPFDRNFARTFDKGEPILD
                     GGNPTAAKYQDIAKKVHTLLDYQHVLDGKV"
     gene            34269..35042
                     /locus_tag="PKM72_02955"
     CDS             34269..35042
                     /locus_tag="PKM72_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNP59770.1"
                     /translation="MKFVCLKCETYMTFEKVEKPAEGSLGVFFECPSCQSRFSMVTNP
                     GETQMVSSLGVQLGGRTEAPKPLEMTRGGLEDNVSAGMGQMAAYLNEKIQGGQPAAAP
                     AASAAPATASAPGTTSEGGGCPFSAMVAQMGLGSTGATGTPAPPAGEQLIWSPDAQEK
                     LAKLPSFVQPMVKSSVETYARKSGFTTVTLQVMDDSKNASTEGIKWTPEAQQRLDNIP
                     DFIRPMARREIERLVKERGQSEITAEIMEEAKEKFMKFM"
BASE COUNT         8006 a         8171 c        10099 g         8831 t
ORIGIN      
        1 ggcgccgatg aattgaaata ctccaagcgc cttggttaaa gtagttcgat tgctctatta
       61 ttgaatccca tgaaaacaca acaccaggcc attggatttt ttcttgccgt catcctggct
      121 atcctgcccc tttcttcggc attgggcgtc cagcatatcc tgggcggagc agacctcgcc
      181 ggccatgccc actcggattt cgatctctgt gattgggtaa aaattcagac gacaggttcc
      241 ctgtcgttgg atgccaattc tttatggtat gggctgtctc gatccggcga tgcggtggtt
      301 caattctcct cacgtttcaa taaccagtcg gttatttcct ccagtgcacc tcgagctcct
      361 ccccaattgt aatactcgaa atctttcagc gcgtatttcg aaaaactgca aatagccttg
      421 aagtccttcc aggatcttgg acagacgcgt gtcttcgttt tgtcgatatc ggaatcttgg
      481 gcgtgcctgc tctagcttcc ttcttcaccc cccgtcgggg gaaatttttt ggctatgaga
      541 caggtagttg acgatccaga taggaagatg aaaggtgccg aagccggcgt aattggaggc
      601 tggtgtggat aaagatgatg ccgaccattc cacaggcaag ggccggcttc acgataaaaa
      661 agatgagcag caatgtcagt aagacagtca ttgtcgtgtg tgtcctcagg gttgatctgt
      721 agaggtgagg gctgaagcta tatggatatg ttcggccgaa tgggagctta gcttgagagg
      781 ggacacagag gatgatccgg agaccctgac catcaatcct ggtattaaat gaccgctgat
      841 tgattaaaat cgaccactcg tcaggttagc ttcgtggaaa taatctatcg gctctatgat
      901 gaggcgccaa tattgggaag gaaaggaaat ggatgtcatg gaacgatgcc atgttgactg
      961 aagtgtccga tttctctgtc tcgtttatcg cgcatgtcag cgggtacgcc atcggcatga
     1021 ttttgtatgc catgttgttg tggctggttt tccaagcgtt gcaggaatcg acccaaaagg
     1081 gtcagagcga accctctgcc caatcaccca gatggcttgc atgttggacg gcaattttag
     1141 gcttgctgtg gaatgttgga gccctgctgg cctttctgct tccgaattta cacctgaccg
     1201 caccgcttcc ggtgttatat gcgttggcgg tctccgcatt ggggtttctt cccgctgtcg
     1261 ttgttctggc tatggttaaa acgcagccca ttggcctgcc tgcctggatt cgtcgatcga
     1321 tttctgcatc cggatttgtg ttaagcgggt gggcatccct gatgcacctg catgctgctc
     1381 taagcgggga atcggggagc atgtccggag cagcgctcca ggccatggtc tggggctttc
     1441 tcgtgctttt gatctggctt cttgctgtca gggtaagggc tgaggggtgg gattatatgg
     1501 ggggaatcgt ggtgctctcc ggattggcca ttgcggtcct tccctttagt cagcatgaga
     1561 ccggggaata ttcctggggc atggaggttg tcggtcatca cgccaacctc ctgttggccc
     1621 tggccattct ctatcaggac taccgctttg ctctgggaga ccttttcttg aagcgggcgt
     1681 tatcgttttt ggtgttggcc ctggtgacgc ttggtgcgtt gatcaccgtt gggattccgg
     1741 tgttacggct cgacccggac aatgaaatgg ccaacctatc ccggcttggt gtttttttgg
     1801 cactttggat catcactgcg ttggcctatc cccatttgcg aaaagcggtg atctggctgg
     1861 tggatgtcgt tgtgttgcgg cgtccggact acgatcagct caggcaggag gtggcaatcg
     1921 aggttaatca ggaggaagag cccgaacgga ttttgtccca tgtcggcgcg ttactccagc
     1981 cagctctgtt ggccagacag attacctgga ggccttggag actggacggc gaggccgggt
     2041 ctgaatggcc gggccaggtc ggctcctttg acggggatcg ggaggaagaa tctttggcca
     2101 gtggagcggt ctccgttgga agcctggtca gaaaagggag cagagggtct tctttggcca
     2161 cgccttgcgg agaagagttc ctcgtggtta ttccaacgat ggaattgcct caatacgttc
     2221 tggtgatcca ggggtttttc catcgaaggc gattgctttc ggacgatctg gtcctgttgg
     2281 aagccgtggc ccatatcatt gccagaagga tcgatgcggt gcgttcggcc cacgagcgct
     2341 gtgccgtcgc cattcgagag caggaaatga gaaaactgac cattgaggcg gaactccggg
     2401 ctctgcgcac ccaattgaat cctcactttt tatttaatgc cttaaccacc attggctatc
     2461 ttattcaaac ggcaccggcg cgggcgctgg acacgctcat gcggctgacg gagttgctga
     2521 gaggcgtgtt gaagcatctt gagggggatt tttccacctt gggccaggaa gtggatttgg
     2581 tccaggctta tctcatgatt gaacaggcac gtttcgaacg gcggttggcc tatcaagtca
     2641 atgtgccgtc aaatttacgg gatctacggg tgccggctct ggtgttgcaa ccactggtgg
     2701 aaaatgcggt gaaacacggt attcaaaagt gtgccaaggg aggggtcatt aaaatcgagg
     2761 ccgaagtgga acagcggaaa aacgaaaatg atcacggagc atggataact cctgagctcg
     2821 taattacggt gcaggatacg ggacatggtc tgtcagggaa ggggcaggag gctgcctggg
     2881 gagctgggat cgggttggcc aatatcagga aacgtttgca attacattat ggcgatcatg
     2941 ccacgttatc catgcatcga aacccgaagc ttggcacgat tgtccacatc cgtattcctc
     3001 ttgttcgttc gagcgatctc ctggcagaag aatccgaatg tcttcattcc gtccagggag
     3061 cgaaagaatg agcggaaaaa tccgggtcat tgttgttgat gatgagcggc cagcacggtc
     3121 atttctggcg acaaccctga agaccctgga tgatgtcgac attgtgggtg aagcggagaa
     3181 cggtgaggac gcgatcagcc tgattcaacg ggaacaaccc gatctggcat tgctggatct
     3241 ccaaatgcct ggaatggatg gcctctctat ggtcaaggcc attaaaaagg ggcatattcc
     3301 gttgattgcg tttgtcactg ctcacaatga atatgccatt gaagcgtttg atatcgaggc
     3361 catcgactac ttgttgaaac cggtcgatcg cttgcgactg cgaaaaacca tcaaacgggc
     3421 aaaggagcgt ttggaacggg atgatgtcat ggcgaatgaa ttggaagctc gaccgcgcgg
     3481 ccaggagggg ggccctccgg aggattcgga ttacatggac cgtctcccga tcaaaaagaa
     3541 ggaagagatc attttcctgc cggtcaatca gatcgcctct atcgtctcgg atggagagtt
     3601 gatgcatatc accacgcttc gaaaggaacg tcatactatc agccaccggc tcaaggcgct
     3661 tgaggccaga ctggatccca ggcgattcgt gcgtttgggt cgcgggactt tggtcaatgt
     3721 ggagtcgata tgccgggtgg ctactatgcc gggagggtcg tatgtcgtca ccttgaccaa
     3781 tacccaacaa ctcagagtca gtcgcagtca gtcacgtttt ttacggcacc aaatcttgag
     3841 gttataagaa gcctgccttg aggaccggtc ccttgccggt tctcccttcc ccctctgccg
     3901 taatccattc attcaggcca atcaaccgct catgaaatta tatgaccgtt cataaaaaaa
     3961 gactgtggat tacgcgcatc ccgcctttat aaattgactg gtattcgaaa tgaaagttta
     4021 tggaaagccg tgtagtccgt tcagtgacag gtttccggaa atcgtaagtc tcaggcaagt
     4081 gcccggacgc tcgaaggagg attggtcctc tttggcccca gaagggtgca tgaggtgtca
     4141 actttactca aaggagggtt ttcccatgtt taaaaagtta cgtagtcaag caggcgcggc
     4201 gttggtggaa tatagtctcc tcgtcgcggg aattgcctta atcggagcgg ccgccgtggc
     4261 ggtattcggc cacaaaacct cggatatgat ggccgcggtc gctgcggtgt tgcccggggc
     4321 acatgctgac gataatgccc cgattgtcgc aggaaaaatc attgaaacca gtgtagatgg
     4381 tggacccggt ggcgatgcca ttgccttgga tattggcggc attgttgcca acgccggcac
     4441 cgaacggcta ggaaatagct ttgggaccac tacgagtatt tcgacattgg ttgtcgaagc
     4501 tcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggaaa tagctttggg
     4621 accactacga gtatttcgac attggttgtc gaagctcaag ctgagtaagc tcccaagggt
     4681 ttgtgtatgc acgcgagtga aaagccttcc cggtcgagtc aggccgggaa ggctttttgt
     4741 ttgagccgtc cattggtaaa aaatagccgt tcattggatg actctgacag tccagaagaa
     4801 tcacctcgtg ggcgagcgaa gcaatctcct ataagacctt tacagtcgga ccatacaagc
     4861 ccttgaagga catgtggggg aagagcggat tgggcctgga gcaagataga accggtgtgt
     4921 agatgaacga gggtataacg aataaaatga aagataatcc agctggattg ccaaagcctg
     4981 tcactctggg ctatcagaat atccggcgca tgagcctcct ggtgagcggg atttttctgc
     5041 ttcttcttcc caccgcaata gcccaaacgc cctcttcgaa gctatcccca aggacaatag
     5101 ttcttaaaaa atccccggag tctcctaccc ctttgcccgt gattgtggcc caacaccgac
     5161 gtgtgttgat tgatgatccg gtgcagcggg ttgcggtggg cgatacgtcc atcgtttcgg
     5221 ctcagttgat taacaataag gaaatcttgt ttcttggtca gcggcccgga cgcacctcgg
     5281 tcattgtctg gcttcagtca ggagcgattc gtcattatca atgcaatgtg cagttggatc
     5341 tttccgttct gcatgcggct ttgcataacg ttcatcccag catcggtgtg accagcgccc
     5401 cggaccgtga cgccttgatc ctgaccggaa aggttcctga tgtgacctat tctcaggcgg
     5461 cggagggaat cgcgcagaaa tatgtgaatg ccagaacggg agaaagaaag gcaggtcaag
     5521 ggccctttgt gaaaagttcc gacgggcagc gggcaaatca atctcagccc ggaaggtcga
     5581 gtcaatcctc gggatcatcc agctcttctt ccgatgatga gggggatgga gaatccccat
     5641 caagcgattc ctccgcccac attgaagtcg cggaggccat cccggtcggc ggcaccatta
     5701 tcaatctgat tcaattggat acgttgccgg ctactcccga ggagcgcatg caagaggcaa
     5761 ttcggaatct tgggggtgag caggtgacgg tgcggcgagt caaacgaggt attgtgcgtg
     5821 atgatgagca ggatgtcttt gtcctggagg ggtcggtgaa aaatcaagtg gccctcttgc
     5881 gtgtgctgga ggtgggttcc cgaatgctga ccggccacgg cgccacgcga catagtattc
     5941 gcgtggtggg tgatgaagcg ggggcgttgg ccggtcgatt gggacaacag caaggggggc
     6001 agcaagggca gcaagggcga atgatgattg gtgggggcgg cggtggaatt ggccagctgt
     6061 ttggaggggc aggtaggtcg gggcgcgtca gtaacctggt gcaatcgaat ctgggccggg
     6121 ccaaagtgat cgaagcggcc aatgggcgca ttatttcttt tcttcatgtg agggatcttc
     6181 cgcaaattcg ggtcaacatt aaactctatg aagtcaatcg gacaaaactg cgatcctatg
     6241 gctcggattt tgtcgggacc tttgacaatt tcgggtcagc ccccgagtcc ggggcccgaa
     6301 acattttggg ctttctgacc ggtacgcttg tagccgacac gtttattcga agcggcgagt
     6361 ttgccattcg atcagtcttc acctatttgg agagtctgga tttggcgcga agcctggctt
     6421 ctccttcgct caccgtgctg tctggagaaa ttgccgcctt tctggttgga ggagaagtcc
     6481 cgatcgagca ggcattttcc ccgtttttcg gcggtggaga tatcactggt gcggcagggg
     6541 tttttcagtt tgtcacgttt cgtgaattcg ggatctccct ccgtgttcgc gcgctggtgg
     6601 gggaagacgg ggatctcacg atcgatgcgg ttccgcaagt ggtgacacct gatttgacct
     6661 tgacggcggg cattgaagag gccacgggaa ccaatcaacc cacgacagca ttcaaaaacc
     6721 ggtccatgca gacgagtgca cgcatgcagg atggccaggc catcctgatt ggaggcctcc
     6781 tctcgcgtga caaatcagat cagcaggact ttattcctgg agttcgtgac atcccggggt
     6841 tgggttggtt gtttcgacgg tttacgcaaa acgacgaaga ggttgaactg gttattgtca
     6901 ttcatcctgt cattatgagg gagccgattc ccgaggtggg cctctgggct tttcccgctc
     6961 tcaccgacta tcgaccggct tgggaattca agtcatggcc ggcacctgag gagaaaggct
     7021 tgtgaagtgg agtgtaaaag ccttgatgac aaaggctcat ccatccttcg gtccggtggc
     7081 atcacaacgt ggcggggtac tcattgaatt tgcctttctg tcgttggtgt tctatgtgct
     7141 ggttgcgttg gttgtggaca tcggaagaat ggtctttgcc gctcaagtgc tccaggatgc
     7201 tgcccgtgtg gccgcccgcg aattggcatt ggtccccctt cccgccgcca tgacctttga
     7261 gcaagcgttg gaggatccaa cggtcctggc tcaggtattc gatccggcct tgctggtcat
     7321 cgatctcgac acgtttggcg atgacgatgt ggcgtttcaa gcgtttctgg atcagttgcc
     7381 catggtcaat aaaatgttga gaccgctcat gattttcgaa caggctgaca ttcaaggttc
     7441 cggccgtcga ttgctgaggt ttcccggtgc gctgttgaat gatccttcgg ccccgagtgg
     7501 tctgggagtg ggaattccac gggtggaatc ccgcggggcg gatggcgtgg aagccattcg
     7561 atgggttccg gttcttgaag aaatcacgaa cggctctttc agtctggtgc cacttgaccc
     7621 cgagaatccg cctcctcaac ggggtctggt ggcggttcgc atcaattatc cgtttcaagc
     7681 ggcgatgttg agcgggtttc agcaaggtcc gggaggcccg tttgaaccga acctggacaa
     7741 tcgcattcaa gcggacgatg aaagcgtaga cgattccata aatgctccgt cgcgcgggac
     7801 gccatttggg gttggtgact tgcctgaagt aggcgtgtac ggcggacgtt ttggccttgg
     7861 aggacaattg gcttttggcg gtgaaacgct caggccctac cggaagcttc tttccgctca
     7921 agccatttcc aggcgggagg tgttcttatg aaaactgccg ggtttcatcc tcgatcgccg
     7981 tgggtcagat tcgtgtcgtc cccgtttttt ttggcgggca tgcttgtcgt gattctaggg
     8041 ggattgtggg tgtccggcct ggtgaaccct tgctggttcg gagtgaattg cgggccttcc
     8101 acggaggggc tggttgccgt tccggtcagc gcgcaaacga ttccggcgtt cacccaattg
     8161 aaccgtcaac atgtgtggaa tgccggaaaa gggcagccgg ctgtcgtgct gcttcccccg
     8221 gaacaggtga ccgaggcaat gaaaaccaat ttaacggaca ttctcggtcg tgtccttggg
     8281 cgagagaagc ctgccggata tgttttcacg gaagtcgatt ttctgccgcg tggttcccgt
     8341 cctgggttag tcggggggat tcccccaggg aagcgggcgg tgcgggtcga agccggtcag
     8401 gtcagcgggt tatatggcct gaatcccgga gatcgcttcg acctgatcgc gacattgccg
     8461 atcgacgcgg atgggaagga caactcattg accatgggag gagtctatgg tcaacaatta
     8521 gcgctccagg cgaatttaca taattgggcc aaacaagcca ccgtgcgggt gctggtgcaa
     8581 aacggtgtgc tggttcaacc gttgacgacc cgggaaattc ctctctcgat gaatacgctg
     8641 acccagggtc ttgtggtcaa aaagaagcct gttcaagaaa tcgtcatcgc ggtggacccg
     8701 gaagaagtgg cttcattgac cgaagccgtg gccgtgaagg ccgagattac gaccattcct
     8761 cgttcaggac atccggacga tcccggggac agcgagacac cggaatccca accctggagc
     8821 ccctttggga atacgttggc tttagggaag tcgtctggcg ggatttcaaa aaatggaaat
     8881 acggaatctt cgctcacgtt tgtggaaacc ttgaacggat cgggagaacg ggagttaatt
     8941 accgtgcctg ttgcgtcgga tcaggagaaa tagtgcgatg ttatacacga tgcgcattca
     9001 tgcttgaacg cgacacggta ctctttggga atgtcaggct atgttggtgc tgttcgtctt
     9061 gctggtttgg ggaatgttgg gtattggctc cttagtgatc gattgggggt atgtcaatct
     9121 caccagagtg caaatgcaaa atgttgcaga tgctgcggcg ggggaaggct tgcgtctgcg
     9181 tgatgtggcc ccagaggatc cggttgccag tgatctcaac cgccgggtgg cggtgagcca
     9241 gctggccgca tggacgtatg atgatgactt tgatctgacc gacgatctcc tgcagtttgg
     9301 tgcggggcct gatgtgttcc tgactggagg acataccgac ctcaatgcga tgcaggtatt
     9361 agacgtccag ccggcccccg tctacaaacc ggtgttgcaa tggaatgatg aagccaatgc
     9421 ccgacatggg gatatggtca gcgggacgtt tgggatttcc gagtccggtt gttcggtgaa
     9481 agccccccac agggaatgtg caaattatac ccggatcgat ttcgtcccgg ctgaagtgaa
     9541 cgccagtccg acggccgatg cctttctcgt gcgattgcgg cgctccataa aaccggaacc
     9601 ctttcaaggt caggccggcg tcgataatgt gccgggagtg agttcggccg gcccgaccct
     9661 gccgctggtg ttaggacttg gctcgcctct ccaggcccaa atcgattcgc ctggaattcg
     9721 ttttacgggc ataaccgtgc gggcgacagc cattgccgat gcccgccctg ttcgaggagt
     9781 gggtctgccg gatagtttat ccgagcctcc ccgtctgggt gttgccccgt ttgcgcttgc
     9841 ccgtgcaggc tggcgggatt ctctggtgat ggatgttccc agttcgggca ccgtggattc
     9901 ttcaggggtc gtggcggtcg ggtccacggc aataggccgg tttctccttc cggttacgag
     9961 cattggcgaa cctgttcttg ctcgatttcc tgaaggggga caggtgacga tcgacggata
    10021 tgcacctctc tacgaagatt ttttggggac ggaacgggtg gtgggatttg cgcgggttga
    10081 aatgaccggc acctcgcccg gttcggtaca gattatcagg cgaccgggtc aagtggctgt
    10141 agaaaatgcc accgtcactc tcctgcctgc gtttcccctt ctaccggaac ccgaattggc
    10201 attgttgctt caagttaatc gtgacattca gaaagaggga gccctgctgg ctccggcgct
    10261 ggttcgttaa ggagtaaacg tgcaatggaa acgaatctcc atatcctcgt ggtagggaaa
    10321 tcccctggac tccgcgcaga attcgattcc gcgcttcagg gggtgggcaa ctaccgatgc
    10381 gtcacggtgt atgccgagtc attccgtcat gcggaagagt tggcgaggaa ccgccggcct
    10441 caattgattt gtgtcgagat gaatggagat cttcgcggct taaaggagtt tacgattgat
    10501 gtgtctcaga tgttgccggg aaccgctatc gccggcttgt acaagcccga tctttttgaa
    10561 tccgatcaat cggaaagcgc atttattctt gaaggcgtcc gggcgcgtat tcaggatttc
    10621 ctccggcacc cgctctccag caccgaaatt cgacaggttc tcgaccgggt ttttgtgcag
    10681 ggaaagacca aatccagcct gtcctcgctc ggccatatcg tctcgtttgt cagcaacaaa
    10741 gggggagtgg ggaaatcgac gatttcagtc aatatggcgt gtttgctggc cagtcgccat
    10801 cctggccagg tgttgctggt ggatgcgtct ttgcagttgg gcgttgccgc ggtgatgctt
    10861 gatctgaatc cccgaacgac cattgtggac gccgttcgtg aaaaaggacg tcttgatgag
    10921 acgttgcttc gcgaattgac cgtgtctcat ccgtgcgggt tgtcgcttct ggccgcgccg
    10981 gggaatgcgg ccgaagcgtc tgaggtggat gaggaatcca tgtatcgtat tttgaactta
    11041 gcgcggcgaa cgttcatgta cgtggtggtt gatacgtttc ccatgttgga tagtgtcgtg
    11101 atgacggtgt tggatctgtc cacctttacc tatgtcgtga tgcagggaac ggcgcccaat
    11161 gtcattggca cggccaaact gcttccaatt ttggaggggc ttggatgtcc ggtcgagcgt
    11221 cagcgtttgg ttctgaatca gaactaccgg aattttgccg gaaatttgac gcaaacggag
    11281 attgaggagc ggttgatgag agaatttcat tatgcctttc cctatcaaaa gaaactgtta
    11341 acctgcgcca atctcgggga accgtatatt ctccaaacta cccgatactt cgggttcgga
    11401 cgtagcgccc atctgttggt tggggaaatc gagcagttag cggaccgggc ggttgccgcg
    11461 gaaattgatc acttggcgaa actgatgaac gaggagccgg cctgaccgtt cccttaccat
    11521 gagcgaacag gattttccat tcccttcgtc atcagagagc cggccgcccg ttccgtttgg
    11581 aaaatcatta agtgcggata cggcctcgag gctcaaggct gcgctgaaac gtccggcgat
    11641 gaacgtggtc aaagacgaag cattgctgga tgatccattg gcgcgagtca agcatgccga
    11701 ttatctgtcg attaaaagcg cccttcagga gcgactcctg aatgaaatcg gagaacattt
    11761 ggccgctgga ctcaatcatg aggatgtatc ggccgtagtg aaggagttta ccgcgcggat
    11821 tttagaatcg gagcacattc ccttgaatca ggcggagcag ggacggctgg ccgaagaatt
    11881 gaccgaggaa gccattggtt tggggccctt gactccgctg ttggccgatc cggctgtgac
    11941 ggatattctt gtcaacggtc accagtcaat ctatgtggag cggtatggcc ggttggaaaa
    12001 aactgacgtt cagtttctgg acgacgatca tgtgattcgg gtgatcgagc ggattgcggc
    12061 gcaaatgggc cggagggtgg atacggcctc tccaatggtg gatctacgcc tggcggatgg
    12121 cacgcgggtt aatgccaccc tgcctccggc gacgatcgac gggccgaccc tctccattcg
    12181 gcggtttggc gtccgtcggc tccggcggaa tgatctcctg ggactgggaa cgctgtcgcc
    12241 ggatatggcg acgtttctgg agttggtggt tcgtggcaag aaaaatcttc tgatttcagg
    12301 tggaacgggg gccgggaaat ccaccttgtt gggggcgttg tcggaggcta ttccctttca
    12361 cgagcgcatc attacgattg aagataccgc ggaattacac ctggatcagg aacatgtggt
    12421 ccggatggaa acccgccagc cgaatatcga gggcaaaggg cggattttgg ctagggatct
    12481 ggtggtgaat gccttacgga tgcggccgga ccggatcatc gtcggggaag ttcgtgggcc
    12541 ggaatccctg gatatgttgc aagctatgaa taccggtcat gaaggcagca tgagcacggt
    12601 tcacgccaat tccccccgtg atgcgttgtc gcggttggaa accatggtgc tttttgcggg
    12661 cacggaattg ccttcccgcg ccattcgaga gcaaatggtt tctgcccttc atcttattat
    12721 tcaggtccgg cggtatgaag acggcgtgcg ccggatcgaa acgattgcgg aaatgaccgg
    12781 catggagggc ctgacccctc tcatgcagga catctttcag ttccggcggc gcgggtttcg
    12841 gggaaagcgt gtggccggag aatttgtgcc gaccggtatc gttccaagag tggttgaaga
    12901 attgcgtgaa cagcatatcg acattcccct gtccctgttt accggaggaa gagatctcca
    12961 tgggttttcg tgaatttgcg gggttgatgg cgattggtct gattgtcggg ctgttcgttt
    13021 ggtggtggag aaacttctct tttcgggtgg ccattcaggc caggcttgcg caggatgggg
    13081 cagggcggga atcccctcag accggtcggt cggtccggcg cgtattccct ccccgttatc
    13141 gtatcattcc cgcgtgtcta ggagtggggg cgagtgttgg cctattttat ttcgtggagt
    13201 tctctccccc gtttgccgga gccgcagggc tgatgcttgg agtgttagga tttttactgg
    13261 agtctattat tgccggcaag cgcttgagcc gcattgaaac ccaattggcc gattcgatcg
    13321 atctaatggt gggagcctta caggcggggt tatcactccc caaggcgctg gatactgctc
    13381 gacaggagtc tgtcgcgcct ctacggccgt atttggataa ccttgtgcga aaaatccgtc
    13441 tgggagaaaa ccccgcaact gcagttcgag aattgggaga gcgggttccg ttggaaacct
    13501 ttcaactgtt ttcgctgacg ctctcggccc agtggtggac aggagggagt ttggccccca
    13561 ccttgtccgt tgtgggaaga acgattcggg accggattga actctcccgg cgtattcgga
    13621 cccaagccgt agaggctaaa gcctcggtga tcggggtgtt ggccatatcc tatgtgttga
    13681 cgttcatcat gtggagggcg aatccccgcc cgttagagaa ttttttcaat agtgacatcg
    13741 gatccttgct ggcgggagca gctatcggat tgcaagcgtt gggcatcgtg tggattaacc
    13801 ggatcagtca ggtcaaattt taggtgaggt cgtcatggat cgaatgtggt atttcggcct
    13861 gattattgtc gcgattgtgg catcagtcat gttgacgccc tgggcgggta tgtttgtcgc
    13921 gcttcttctg ctgttgttcg gaatggtttc ttgggcaagt cgtcaccgga gtgttgagcg
    13981 ggttcgcgac actctggagt cggaacgttc tgttctggat cttggacggg cacgctccga
    14041 gtggtggctg cgccggtggt tgtttttgtc ggggtttcga gggcgattgg cgcccgtggt
    14101 ctttatggga cttaccggag gagcgctagc tctgggaatc gcgagtgcat atatcatgaa
    14161 ccggtctgga gtggtcgatg tgatggtggt gccactgcag ggtattccgg gaggaatcgg
    14221 ggaggctttt atttccgtgg cctatgcggg cccctggatc gtgctttccc tgttagccag
    14281 cattccattg ctggtggttc gtgcgtcccg tcgccaacgg gtcatggatg tcgaccatga
    14341 ccttcctttg atgctggaat tgctggccac gctgacagaa gggggactga gttttgatgc
    14401 ggccctggat aaaatactcg gggcccagtt tcgcaccagg cctttgtcgc aggagtttcg
    14461 tacctaccat cgagaactgc agctgggtgt gtccagattg cagggtcttc gacggctggc
    14521 ctaccgtttg gaagtgactt ccgtctcgtt cctgatttcc gcattgattc aaagcgaaca
    14581 agtaggggca agtattgcca cgacgcttcg ccgtcaggcc gacgatctcc ggaaccgtcg
    14641 ccgagagcag gctttgatgc ttgctcaagc ccttcccgtc aaattggtgg ttccgctcat
    14701 tctttgcttt ctcccgggaa tattcgtgtc cacgctcggt ccctcgttac atcaactggt
    14761 gaaccttgtg agcgggatga cgggagggat tcggtaatgg tctggcctgt gaggccctac
    14821 acagcagagg agtcgacgtg aagttgtggg ctgcctatgg gttgatagtt ggtctgcttg
    14881 tctgttcggc gtgcgccgat gccccttatc ttcgaaatca atacgaaacg gcctgcacgt
    14941 tacccgatct tcaactggcc gaagcgtggg acttgctgga ggaagcccga tcgacctctg
    15001 gcggatgcga ccgcgatgcc cgcggaatgg atcgttgccg tgcgctgcgc gctgaaattg
    15061 aaagaattgc gtttgtttgt ccaaaccaca ttccttcttt gatgacgacg gctgttctgg
    15121 cctatgagga cagggacttg gggaaagccg gggaacggct cacatccatt ttggatttgg
    15181 tcaaagtgca tccggatgcc gcggtgttac gggcacggat tgctctggaa gaagggaatc
    15241 ttccgttcgc ccttaccttc ctgaaacagc gcatggagct gtctcctgac catgctcagc
    15301 tccgggaggt gtatgcggcc accctgtttc tttccgggcg acttgaagaa gccaggactc
    15361 aattggcggt ggccgaacaa ctcggggccc ctcgatggcg ggttgcatat catctcgggc
    15421 tgtttgaaga acggaaggga gagctacaga aagcgcgcct cctgtaccag gaatctgcgg
    15481 atttaaaggc tgattggaat ctacccattt cccggttgca cgggctattg gccaagcctc
    15541 tttcagataa aaagccgtct gctctggatg gaggttattt cgagcttcct tcgcttctca
    15601 agtagaagca tgttgtttcg gctacgaaac ccgcgctctc cctggccggt cgcacatcaa
    15661 agttgaggag gaaaaatctc aagtgtctct cttttcttga gtgattaagg acaggtgctg
    15721 cccttttact ctggctcatg tgttgcgtgg ttgtcccttt ctgttgcctg atgtttggag
    15781 gcagaagtca tgttcgggca agaccaggtt gaatgattcc cctctattgt taaagagatg
    15841 ccacggcatc ccggatttgt gttgttgcgc gaaggtgagg gtcttcccct ctggttgtcc
    15901 atgtttcaac catggatggt gtaatgcata ggctttgccc aaaggtgaga gtgatgggat
    15961 gacgcttagg ggtcttagca tctgggggcc agacctcaaa acttccatca attttgaccg
    16021 ggataatggg gatgtgtaat tcacaggcaa gaaccccgag acctttttta aagggtagga
    16081 gtcgtccatc aaggcttcgt tctccttccg gaaaaataag gagacttttt cccttccgga
    16141 gaagggcgac tgaggccttg agaactgtca ccatgggcgt ctccggcccc acgggaatca
    16201 catgacccac gcgtgcgacc catcgacgaa acggtgatcg aaaaaagggt tcccagccaa
    16261 gagtgtaaag ttgtgaaaag gtggatggtg gcacagtccc caaaataata aagggatcaa
    16321 taaaactcag atggttggcc gccaggataa aaggtccctc tttgggtaga tgtgtgaggc
    16381 ctttgacagt caatggaaag actatgcggg agaagctccc tacgatagcc attcctattt
    16441 gtccgatgat tttattccct ctggaaagcg gagcgaggac tgtgtttgtc agggctggag
    16501 ggagtggggt ttcaaagatt tgatgccagg aggaggtggt ttctgtctgt gcggcgggaa
    16561 tttgctggag ggccatgagc ttggtaatca catcacgcac cgtgacgatt tctcccatga
    16621 acgtttccgg gaccggcccc aaccggcttt cgagggcggc ttgaaattcg acgcgtttca
    16681 acgagtcgaa tcccaagtcc agatccaggt gatcgtccaa tcgtagggcg cggtctgccg
    16741 aaatcagcct gttaagcgtt tgaataaccg cttggcctat ggttgtttcg cgaatctcct
    16801 ggtccgtctc aggttcctgc tccggtgcgg gttcaacggc agtctgtttt gtttgagtca
    16861 tggcaagaac aagatgccgt tggattttcc ctaaacgtgt acgggggagg ggatctttga
    16921 tgaaggtcac accactaatc cgtttgtatg ggggaagggt tgcggcgaca ctattgagtg
    16981 catctttgat gtaggtggcg gagtcatgga ttttgtgttc tcgtaaataa tcaaaatttg
    17041 gtacgaccac ggcatgaaga tgttcgcctt ccccaccagc acgggggagt ccaaggatgc
    17101 agagttcggt gattgcagga ttctgtttgt aggcttcttc caattcttcg ggaagaatgt
    17161 ttttccctcc aggtgtgacg atcaattcct tgattcgtcc ggtgagaaac aggtatccgt
    17221 ctgagtccag atagcctaga tctccggtat gaaaccagcc atcacggata gcctcggccg
    17281 tttcagccgg cgcttggtca tagcccttca tcacattggg tcctcgtacc accacttcgc
    17341 caattccgga ttcgttcggc tgatcgattc gcacctcgac gctggcgagt ggtgggccga
    17401 cggagcccgg tttcaggcga gacaaggaac taaaggcaac cacgggcgct gtttccgtga
    17461 gcccgtaccc ttcacgaacc gtaaatccca gattgcctaa atcctttgag atttgtgggt
    17521 cgagtttggc tccgccgctg cataaaaggt gaagcgaggg cccaaaccgg cggtgaacag
    17581 gagcaaaaaa ccgtcgtccg ggattccatc tggtatgggt tctcacaaaa tccgacaggc
    17641 tgaggagaag cctgatgatc caacggacca atcccggacg tcggtcgagt tcttcaaaaa
    17701 tcgatcgacg aatcatggta aaaatttgtg ggacccctac ggcgatcgtg atcccggtct
    17761 catgaataca ttgcaccaga tccggcccct tgagtgatga taggaaggtc attcttgcac
    17821 ccagtaatat gggaacgagg taggcgatca tgaagggata ggcatggtgc agcgggagca
    17881 tcactaggaa gttatcttcc ggccccgcta aatcttttcc aataatgtct ttggcgttgg
    17941 ccatgaaatt ccggtgagtg agtaacacgc ctttaggttt tttggttgtg ccagaggtat
    18001 aaagtaacga agccacgtcg tccggattca cccgcaggtc aacggagggt ttttcctgtc
    18061 cttggtccat gaggtccttc atggacaggt ggtgagattc tgtaagggaa tccatgttca
    18121 cgacggtgat ggtggctgga aggtctcgga tcaggtctac ggtttttgag ctcacgaaga
    18181 caaaccgact gttggacgtg gtgaggaagg acagaagttg ctcctgtggc atctgaatat
    18241 ccaggggaac ggctgtggca ccgacggtga gaatgccgag gtgagcgatc acccattccg
    18301 gacaattttc agccacaatg gcgacgcgat gccccggatg aagtccatgc tcatgaagac
    18361 cgctcgccag tccccgaaca agcttcgaca cttccccgta ggtttgttgt cgatagactt
    18421 ctccttcttt tatttgcatg accacatgat cggcggcgat tgccgagagt gcgaaaaagg
    18481 cctcaggaat cgtatctgga aggtgagtgg gatcgatgag aggagcctgc atcgtttgag
    18541 tctcctaaaa gggatgccaa gaaaagatgc aaaagcaagt ctacccgaaa gggggcatgg
    18601 ttccaagaca agacttgggt agcggaaacc gactctaaaa aagcggcagg taggtgtgat
    18661 ggggattact cagcaggaag agcgaagatt taatacccag gaggcaaata caaaagctta
    18721 acaaaaaagc aaaaaaatcc gaaaaagcca ggaggtttct gtttaggcgc ctggtgtttt
    18781 gcggtcactc cctaacaggt tattttgatt tattgttgaa aaagggattg ttctatgccg
    18841 ttctttaaag ttgaagatac ccgggatctt tgcgtcggga cgtttgtcaa actggaaggg
    18901 tcctggttca gccatccttt tcccacgaac acctttcaaa tcaagtcctc tgatgatctg
    18961 gccaccattc aagccctcaa aaatgtcgtc attctccaag atcccgaccg gtccgccttg
    19021 ttagataccg aggaggattc agatcaaaac cggcggccct tgaatttcga ggagagtgca
    19081 agcagggagg aagatacaga ggccattttt gattcagaat tggatcctcc cgcagacagc
    19141 ggaccactta ttaaagaatc ttctctatcg acaatcctgg atcggcgaga ggattaccat
    19201 gaatttcagg atcatttgcg caaggtcgaa ggggcatatc aaaaaaccct tggacagggc
    19261 aatgagttgt ttcggcaact aagtgaacgc cgaccgtctg gacgtaaaac cgcggatgcg
    19321 atgatcacca gtattgtcac cgccatacaa catccccaca cggccatgtc tttaattgat
    19381 gttgtcggta gtaacggtgc cacgtggggc ctgtccgaac atgcccttaa cgtgtgcacc
    19441 ttgtccctcc tcattggccg tcaattgggt ttggaagccg aaggattgct tgatctcggg
    19501 cgtggagccc tcttccacga tgtgggttat cgcgcgttac ctatgaacgt acgatttagt
    19561 gcggggggca tgaaaatcga atcggatccg gagttgggga aacgtcatac ggaggtaggg
    19621 cgacagctca tggcggcctt cccggatacc agtccggcag tattggaaat gatcggtcac
    19681 catcatgaac gactggatgg ttcggggttt cccaaaggca tgagggcaag tggcctctcg
    19741 ttgccgacca aaattgtcat ggtggcagat cattatgatg aactctgcaa tgcccctgat
    19801 ctgcaaacga gtctcactcc ccatgcggcc ctgtcccgtc tctttcgcca cgtcgtcatg
    19861 aaggaagaat tgtccagatt ctgtccggag gtggtgcaag ccctggtgca ggccattggg
    19921 gtctatccgc caggaagcct ggtagaatta acggacggat ttctcggggt cgtggctcgg
    19981 atcaatattc atcaccccac aagaccgaca gtgttgttgt atgcgccttg gatttgccga
    20041 agtgatggaa tgttggtcaa tttggcgcaa gagcctgagt tggaaatcaa gcaagctctc
    20101 catgtcaagg atgtaccctt gaatgcccta gtgtatttga gtccccgtcg aatggccatg
    20161 tttgtgcatg cgaccgaacc caccaccatg gcgttcaaca gcaggcggcg gcggtaatct
    20221 cccaaaccca ccctgaagtg tgaaccggag ttgatgtaga gccggataca ttttcttcag
    20281 aaatgaagtc tgtctctaac cactgtctag tatcgggatg gccttgggcc atgcttggaa
    20341 atgtcgatgg atgaaaagaa attcttcata ccaaatccga cgccgactcc aaccatattt
    20401 aggctccgaa taaagctaac cggaatttca tcctgcggtt ctcaccggaa ggatttttcc
    20461 cctgaaaggc gccttttcca tttccttgga gaaacaataa tccgaggcga tgaaatccgg
    20521 accaatcaac aaacagacca gatcgacctg ttctaattgt cggaggattt tcgcctccca
    20581 aggctcgccg ggatacaatt gccggtcgtg cccgaactcg aggggaaaaa cttcgtttta
    20641 aaattttcaa acacactttc atccgttcca aatagggatc ttgattcttg tggggatagg
    20701 aaatgaagac ccgcacgcgc cgagtcgagc gggacgggtc gggaaagact ttctgatcca
    20761 tatcaagctc cggtaactgg aggaacccat gatagggttg cccctccaca atgtcttgtg
    20821 cacgggaaat cacctgcggc tttcccgttt tgccgggctt gctctaacac cgcaagcgcc
    20881 acaaaatcct ttccgtccag actcagtggt gcaagcagtg cgaggtcagt gagctgattg
    20941 cccgcgcagc cgaggatggt caattctttc agagtggcga gcggtgcggg gtcggtgagc
    21001 cgattgttgc tgcagtggag ggtagtcaac tgttccatgg cggtcagggg tgccaggttt
    21061 tgaacgccta ccttactaat attccgtggc gcaaccctgg aagctgggca aattcctgcg
    21121 gaagaagagg aatggttccc tttggaccct tccaagattc gacactctgt tgcagggcta
    21181 gctcttccag ccaggtcagc tcgaagagct caggcgggag tccttcccag atgaggcagg
    21241 acaggtccag cgacgcggcc agggtgtctt tggcctctcg tatgcgccga agagcttgtt
    21301 gatagtctcg ctcgatttga tctggattca cgggagtcca ctcgaccaag ctgattttaa
    21361 gtttggaact ggaaactccc ccatttgaga ttgggcttag gttttcccaa ctcgatcgtg
    21421 gaatgagtgc agattagcga gcttgttgtt ttaaccagcc tttggcttcc gggtaaaatt
    21481 ggcctagaat ttcttggctc atctctgaga gtagaggagc gaggttagtc tggaggtgat
    21541 cggtaattcc ggttaagggt tgcggagatt gggtcaattt ccagagttgg tgaagatcgt
    21601 cctttcgttg ttcaagagct ttttccgtgg cgcgttgcca aaagagggga aactctcttg
    21661 ggtcagtgta gtcacccctt cccctcccgc aatgggtcac cgctaaatag cgtagcaacg
    21721 catcgcgtgt ttgccggtcg agaaaggcag gaacccagga tattcgattg gaatcttctg
    21781 tttcctgact tttggcataa gtccgttcgg ctaatgctcc aaggatggct ccaccaatgg
    21841 taaaggcgcc atgagagagt cctccggtgg ctaagtctga ggccaatccg gcggcggctc
    21901 cgctggctag tgctccccag aacgtttctt cttcccatgg ttttctttca cccggcacga
    21961 tcacatcttc aatcctggac ttgagtgtgt gtgggacatt gccggttaac ccatgtaact
    22021 cgatcaggtc tgccgtgacg gctgagatac gttgggctaa tcgttggtcc agggcgtgaa
    22081 tggcattttt gatttgcggc ttttttgaga gccccgtcgg ctcttgggaa aggcgttctc
    22141 catccaaggc ggtctcggct aagagttgag cgagacgggt catggatgca tcaaagatct
    22201 gccggtgggt ggcataccag gccttttcta attttttcat cgtgtgatgt ttttccgagg
    22261 gcaaggcctg agtgacgtga tcccataaaa caccttcttg aacccagcaa cgcgtaaagg
    22321 cgtccaagct catcacgtct ttaatgacgc gatgattgac ccaatgttgc ctccaaaggg
    22381 attccaattg tcgctgttct tgtggatcga tcaggccggt ttgattcact aacacaatga
    22441 cgggcttatc gagccacgtg agaagatcca gttcaggctg aagatagccc gccatggacg
    22501 ggtcctctgc agcattgacg aggtataaca cgacgtcagc ttcatccctg acgtttcgga
    22561 tcgcttgctg actgcaccaa aacggtttgt ctgctatacg gtcccagact tgagacacca
    22621 tccacccaat cgggttggtg agtccttgaa gtcgacccaa gagcttgtgg ctatttccaa
    22681 aacctggggt atcccacagg ttgagccgtg agccatcaga agtctccagc aacacaaagt
    22741 gttcattttg aagtgtgaca tgggcctgat cgagcacctg tccgatgtcg cgacgcaaca
    22801 gagtgcgggc caaggtggtc ttcccgatat tcgtgtggga aatgagtgaa agagtgatgg
    22861 aggaaggcgc cgtcatgtca tctcaccttt tgggggccaa agagcagctt gtgctttctc
    22921 aagaagctgg tcgcggaggg tgttctttgc atcgaacatc acgatttgca ggtggtggtc
    22981 gttggcgagt cgttgccagg tttgttgccg tcctgtcatt tgtgcctgat cagccaagtg
    23041 gcgatagggt tcctggtcca gaacaaggag taagtgatgt tgtccgttac agggttcgat
    23101 tgaggtttga agcatatgga atagttcacc gtgcacctcc gcttccgggg tttgcgccag
    23161 attacaaacg agaaccatcg tttggggtga ggagtttgtc tgcaaccagg gaacacttgt
    23221 ctgtccataa ggaagcgggt ctctccaatg caaagtcgcc tgattgccaa ggaggtccag
    23281 gaggcattgt cccaagaaat ccaaagtggc cggggagggt tgataactgt acggcagaat
    23341 gtcaatttgg atgccttgtc cgcgttcagt actcagcaac tgcagatagt acggctcatt
    23401 gagattgagc gggatggatt ccgcggcttt atgtgcaaaa cgtgcggata gccaggccag
    23461 gattacccga ggtatgacga ttcccacaaa ggccgtcagt gcccacatat gcatccacgg
    23521 agcagcagag ccatggccgg gagcttgaag atctgcaagg tctacagctg atggaaaggg
    23581 aaaacccaga agccaggctg caggtcccaa gagtaggtgt aagaccgtat gcacctgtgt
    23641 cgctgataag aaggtgcttt cccaggtggc ttgataatct aaaactagtc cacgaacgta
    23701 catactgagg atgatgccca gcgccagaca ggctgatcca atatgcagca gatgacgaac
    23761 gcgactgata atgatgggat gacagtgttt ccaccatagg ttcataaatc gttcggtaga
    23821 ctgagggatc cactctctac cggcacgttc aggtaaagca atctttggcc atggtccttt
    23881 cctccaccat ttggttgtgg tggcaaatcc ttttgacagg tgccggagaa gcggcccgtt
    23941 tgggtcgttt ccaagaagcg gcagcagaat gttaccggta taggtcccaa tgttccataa
    24001 aagcagaaac aagagtggaa aatttaagag attgacttgt tgagatgttc ctagtccatt
    24061 gatgagaagg ccggcagcta aggccggtat cgccacgaaa gtgaatggaa tcgtgacctg
    24121 aacacctcga aatgactccg tgatgatggg gtaagaagtg gagagaaaat tccagaggga
    24181 ttcagcccga tttatgagaa attgtattga gctttcagta gatgggtttg cctggtgaga
    24241 cgagtgtgct tttgcttgtt gagtggcccg ttgtctggtg gcatgtgaga tgtaacgtgc
    24301 ctgcgggtct gattgttcca gcgcctggat taataaaatc tttgatgcgg ctctcgaatt
    24361 catgactgga gctattttgg aatctgaatc ggtgacattc ttaggatgtg gtgactatgc
    24421 aaggacggag ttgacctacg atcatgactg atttgactgt tgtgtgggtg taggaagtga
    24481 tgttttctat tgagtgaaaa atcgctaatt gcatggtgtt tggtcgagcc aaggaaaata
    24541 ggaaacacca ggcatgctcg ccatatttga gttaggaatg gagtgaaacc gcacctacct
    24601 ggtgtgactc agaaggagaa gcatgcagtt gagtctcatg agattggcaa ttttcaccac
    24661 catggtcttg ggaaaggaaa gaaatattcc aaccggtcct cacacctacc tctaaaacac
    24721 taaagacaga aacacaaccc cgagcccaaa aactctcagg acgacaccaa acatcgccag
    24781 tggtgatgcc ataataatgg caaaaaaaat cgatacaata agggggacga ggatgggctc
    24841 tgccgtccgt atcccatcca caacaatgac aaatattggg ggagttagca agaatggccc
    24901 agctgggaga tgggtggaag tcagtggttg agttttctgc tggtttgggc ataattgaaa
    24961 attgaatcct gaaaaaccct tccatttttc aggtatgata cccgtttctt tccttccctg
    25021 tcttcgagaa cagttgttgg tgctatgaaa aggaggcagc gggaaaatga aatttcaaga
    25081 ggctctatac tcttagagtt gtggttgggg cggagtccag gatgaggggc ttgagggcta
    25141 ttcctgaatg gccttccttc gttgctggag atatgcattc cggacggctc catataaatc
    25201 gagcgtgcct tcctcaacgc cttgaaattt ttcaagtgtg agtgcccgga gattcacggt
    25261 ttcgccagca accattccaa gccgtgaaaa tgtgacggtc ggatcgtgtt ggaccaacct
    25321 tggggcatca gcgatttcaa taaagggaag gaccagccaa ttaaaagggt caaggaagga
    25381 gtcgccgata aaaccaatcc catcccgtaa tgtgaagggt cccagaaatg gaatcatgag
    25441 gaagggtccc ggccccactc cataggatcc taaggtttgc cccatgtcct cttggggggt
    25501 atccaaatca aacatgattt tcgccggatc aaacaaccct cccaccccaa gggtggaatt
    25561 gatgaggaac cggctcagct caatgccggc tccctgaaac tttgcctgga aaaggttgtt
    25621 gaaaagtctc ggcacaaatc gaatattttg aaacacgtta gagaagcttt gctgaacgtc
    25681 gggagggata aagaaattat aaactttggc agccggcttc accacgtatt tgtccagtcg
    25741 gtaattaaat tcaaagacgg cggaattaac aggttctaga gggtcatagt ccttgccctg
    25801 ggcttgatcg gattcatcaa agggatcaaa aaattcagtt tctttctcct cgccctccga
    25861 gtggagtccg tcctcaaatt caaccatttg aatagtcggc tctgattcca tgccaccctg
    25921 ggtcttggtg gatttggcat ttcctgcaca tccactaagt cctacggtaa ggcatagtat
    25981 cacccagatg gacaatcgca tcgtttctcc tctcatgcca aaagcttctt ttttgaagat
    26041 gtaggtggaa ccagtccact tcatctagtg attagaactt taaaataatc cagtaataca
    26101 gaataacttc aattatttca gggataaacc gagcggactg taacaaaatc ccttacccaa
    26161 gccaacaatc tctttcctcg tccccacttc tcgaaagatc tgtgactagt gataggattc
    26221 aggcctttct cgtctgtttt ggtaaaggca tgaaaaagac tgctgaaagt tttgtcagat
    26281 tccttaacaa aacattacag agaaaacctt ggaaaactca gatgctgcca ttctttttca
    26341 tgatgttcct cagaggtcgt aaagtgcatc gacgttttca atgaggcttt tctctttaga
    26401 gggaagatcg tcctcagtat tggagaggag gcagtattaa cgaatatcaa agtgctgcat
    26461 gtgaaaaagc ttttttagtg gcactccctt tgctggtttg tcgtaagtga cccatgcggt
    26521 tcacggggca tcaatgcctc ttaaccagaa ggtcagatga cctgagaacg ttcaatagaa
    26581 gggagatgag ctatgagccc atcggtatcg gaacagaagg cttcctggaa tggttccatt
    26641 aaacggggga aaaaacagga ccgtcaccct gtctcgtccg gcggtggctc ccctgatgtg
    26701 gataggtcgt caggaataag tgacaagcaa cttcccctat acctaaagga aattgggcaa
    26761 gtgtcattac tagaccgtga tggtgaagtt cgtctgtgta agaaaattga ggaagcccgg
    26821 cgaagcatgc tggatattct ttactccctt cctatgacgt tgagctatct tcaagagcag
    26881 cgtatgcggt tgatgaatgg cgaaatcgtg gctaaacaca ttgttcaaaa ggagaaagag
    26941 gagtatgtag aggccgagtc ggaggaagaa tgtgacattc cagacatcca agccgagcag
    27001 ctagaagatg aagaatttcg tcaacgggtc attcagcaac tgagtcatct ttgtcactgc
    27061 gttgaattca tgatgacgaa ggacaatgac tccacagcta tgcctggcgc ttcgacctct
    27121 gtgcaagcaa gaaaaaaatt atggcaaagt cttgaaggag tgaattggca tcctgcgttt
    27181 acgaaacagg tggaatgccg catacgcgca atcgaacgtc aacttgcaga gttgttgaaa
    27241 cggttaggaa tatcctcccg agagattaat aatagtggcg aggcaacaaa agggatgagc
    27301 cgggaatctt tctctgtcag gaacacggtt attgaggcca cctcaagttc ccatcgcatg
    27361 gcccgggaag caggggaaca cctcgtatat ttggagcaag aagttttgcg catgggcttt
    27421 tccgaatttc tcgcacgggt tgggcaatta gaccaagcca agatccgtct gcatttcgcc
    27481 aaggaggcga tgttggaggc aaacttacgt ctggttgtta gtgtggcaaa acgatatgtg
    27541 aatagaggtc tcgatttgct ggacctcata caggaaggca atattggcct gatgcgtgcg
    27601 gtagatcgtt ttgagtatca gcgaggctat aagttcagca cctatgcgac gtggtggatt
    27661 cgacaggcgg taacccgggc attagccgat caatcaagaa cagtgcggat tccggtacac
    27721 gtgtgtgatg tgcttactcg agtgcgacga acgttggaac gtttagcggt acaattgggg
    27781 cgtgaaccga aattagagga gctcagcggc gctgtggata ttcctctgga taaactgtcc
    27841 accatgttgg aagcgacaaa agggactctg tcgctggaga ctcctatggg cgatgaagac
    27901 gggtcgcctc tttctgatct cttgcaggac catgccgcag tttctccatg ttattctgcg
    27961 gaacggatgg atctacagga aaaagtgact accttattag ggacattaac ccctcgggag
    28021 gcacatatta ttcggcgacg cttcggaatt ggagagcttg aggatgccac tcttgaagag
    28081 atcggcatag aattttctgt gacccgagag cgaattcgac aaattgagga acgggcgtta
    28141 accaaattgc gtgaacccca acgaaatgtg gtcttgcgta atttttttca accatccggt
    28201 ccagccctag aatagccaga tccttttggt gaacgtgcta tgtcgaacca caaaggtggt
    28261 tgatttcaga atggtacaat ctaggaaagg ccattaagga aaaagcgaat caccggaagg
    28321 tggggaagat tcagtcgtcg aagattcgtg gtgacccaga ctacctttga aattagcacc
    28381 cgctgtctgg gcattgagga aggtcgttcc attaagattg gcctgctgga aatcgctccc
    28441 atacaggtca gtgtcctgaa aatctgccca agcacagtcc gagcaaaaaa agtttccatc
    28501 ccgaatttga gcacggacga atgtggcgcc gcgaagtgtc gcatgctgga actcggttcc
    28561 ttgaaggttc gcatgctgga aatccgccca tataagatcg gcttgtgaaa aattcgccca
    28621 ggctaattgg gcacctctga aacggacact tcgcagattc gcgccttgaa aatttgcgcc
    28681 acgcaggttc gccccctcaa aatttgcccc agggaggtag gcttcttgaa aattggcatt
    28741 ccgaagatcc tggtggcgga agtcggcctc gcgaaggtca atctgaaaaa aatcttggga
    28801 acttggtgga gctgtgtggg tagactgttc gtgctcagat tgtttgtctt ttttgattcc
    28861 agcctgctgg gagcgtctcc aattctgatg ggtgtggaga agaagggccc ggtcatcttc
    28921 aacttcgttg ggaggaggct gcaagggacg gtcttcaatt gatgccataa agagatgcct
    28981 tgaaaagaca acagaaaatg aaaaatgaat tgacagggat caatgggatg tagatcacaa
    29041 tgaaacctta gcgaaattga taacaagggt ctagtccaaa aagcgggtct ggagggacca
    29101 tggaagcatc aatatggtat acaaatactc ctgcgctttg gacatgaaaa attatcgaag
    29161 gatgttccaa aatattttat tggcggatat tacaaaaatt agacatgtgg tgatgtgaga
    29221 tggaattgac catttttgtt ttatggacgg tggtggcgct attgggctat caactgtggg
    29281 tgttgtggaa agcgcatggg tcatttctgc agattctgaa ggaacttcct gatcgtattc
    29341 gatctggctt aggggtggta ggcgttagag agctgaagga cattcaagaa agattttatc
    29401 gtcttgagca acgggaccat attaggaaga ttccagagac aacgggcgaa aaaatcgacc
    29461 agcattttca agaatgtttt caaaaagtgg acccccctgt tgattgcctc gagcccattg
    29521 atgcctcact ctttgccatg gacggacctg tcccccgttc caggtcgatc atgtggaaag
    29581 gccttcgctt taccctcagc gattcgattt ggttccatgc aggagtggtg tcgaacgaag
    29641 atgtggaaga tgatcaagtt caatccatgg tgcatggacc attttgtcag aggtgtttga
    29701 agcgattggt ggggaggatt cgtggtcagg attcgggtgt tccaccgcaa tgtcacaact
    29761 gtggcctatc ctggaggata catgagcccg actatacaga aatgcccctt ggtgatctca
    29821 agcgcatggt gtatgccatg cttgatcgaa aagtaagaat aagtcgaaac gttcaatgcg
    29881 aagttttcat tgtgggggag gaggattcag gtgctatcca gtgatctttt gccgacagac
    29941 agatcttgtg tgggtgtagt tgggatgcta taaccgacca ctatgctcct ctgcccttcc
    30001 tgttcacagg agaatccaga taccgcgaac ttttgtttgc agtgcggaaa agcgcttggt
    30061 tccccacctt cagagactgt cccagaggtc aattctgaag aacccggggc caggacacct
    30121 ccaactcaat cccaattgtg ggaaacgttc atcggtccaa gtaaatccat tcaattttcc
    30181 gtcaagaccg ggtgggtgtg gcggtcggct tttttgtact acaaagaaaa atttgaaaat
    30241 attgaaaccc ctcaaggtcc acgatttgcg ttaagttgga actggcccgc cgccctgttt
    30301 gattcgtttc tttggttttt atatcgaaag atgtacctgt ttgcgttgtt atacgctgta
    30361 gccccggctc tggccatttt tatgactggg gatgtgatgg tgggaattgt gtcaagaata
    30421 ttggcaggag ccagcgcgaa ttacttgtac tattggcatg tgaaagacaa ggtacagaga
    30481 attatggcga tgcccggtct tgatgatact ggtcgtgctc acctgattca ggaggagggt
    30541 ggagtacaac cttatgtcct ctggttgggg gtggtgctgc atatgctcat gtttggttta
    30601 ctggcggcga cccttgttca agggcctccg gttgaggatc ctcttggtgg aacggaagga
    30661 cgtcctgttc aaaaattctt ttaagaggga gaatcgggat tcttgatggc tggatggccc
    30721 tgggattgat ttgaggcggt ctgccaattg aaccaggctc gaaaccaatc ataatcagat
    30781 tggtaggcgg tagatttggc gtccccctgt ttgccttttt cagtcaagag ggtgtaggtt
    30841 ctgggccaat tttttgtcca ccaggatttg agggcttcgg gtgaggaggg ctgatcgtct
    30901 gactgaaaaa tatctgcggc atcaacaatt tgggtgatga gcttccacgc tcgatctttt
    30961 cctaaaccca aatttcgaaa atgttcccgt aataaaaaga ctacccataa ttccgcactg
    31021 tgtttcttgt tgtgtttaaa cggttgtgtt tgacgaaggg gaatcgggtc aaaatttttg
    31081 atgattgagg tataatcggg gcctccgtac tctcccacca attctgcaat accctctaac
    31141 atgttggcca attcgacctc aacttccgat cgaggggagt ccgcaggcat ggccagcgga
    31201 ctcggtgttg tgagcatgtc aatgacgggc ttgagttctc tgaggcgacc gactgcagtc
    31261 ttgaggatac gttccgactc caaccaatag tcagaatcgt ttttggcagt tcgttcttgt
    31321 cctgccgggg gaaggactgc gggaatccaa tgccggatga gtgtaaatag tatgtagtcc
    31381 agatcaccgc cttcctgagc cgctcgatcc aaggcgtttt gcggcccaag gccttgtcgg
    31441 aaaaactgtt ctgttcgatc tgcgacgcca tgtcggccag ccaatgcggt ccaccagcgt
    31501 gcgaattgtt ccaatttatg ttcgtcagag accatatcgt atcgaaagca gggaaaaaat
    31561 tttctggtca tggtacgatt cggtgccaag caatgcaagc aagaataggt cactgaacaa
    31621 tgttgaagag gcaaccacga aagggagcga gggaggaaaa ctagatggct cagttggtgt
    31681 taattcgtca tggcgaatct cagtggaatt tggaaaaccg ttttacgggc tgggtcgatg
    31741 tcccattaac tcctaaagga gaacaggaag cccgcgatgc tggtaagaaa cttaagtcct
    31801 ttcagtttga ttgtgcattt acctcgatgt tgatgcgcgc caaggaaacc ctccgattgg
    31861 ttctggaaga aattggacag acgacgattc ccattgagga aaacaaggcg ctgaatgagc
    31921 ggatgtatgg agagctgcag gggctcaaca aaacagaaac agcacaaaaa tatggcgacc
    31981 aacaggtgaa aatttggcgg cgaagctttg atgtgcctcc ccctggtggt gaaagtctga
    32041 aggataccgc tgaacgagtc cttccctatt atgaaaatcg aatccggccg gctctattgg
    32101 cagataaaac ggttttaatc gtggcccatg gcaatagttt gcggtcatta gttatgcaat
    32161 tggagcaatt atcaaaagaa gctgtgcttg aattgaatat tccgacgggt gcaccattat
    32221 ggtatgaatt ggccacatct ggggaggtaa ccgaccatcg atatttgtaa ttgagctaga
    32281 gcgccttgtt gaaatctcta aagcgcaatt tcaagtcatc atttgccgga gaagattgct
    32341 ttatatctgt ttggttgaat catggtcgtc attttccttt tctttattgc agggttgtgg
    32401 atggccgatg gtgtggcctt gttgattgcc ccggaacgca tgattgccac cttgcgacag
    32461 tcgctgattg tggcaccagg gtttataaaa tggggcggcg ttgcagccct attgggaatc
    32521 cttctgttat tcggaaccag aggcatcccc taccaacctc tttggatcgt cgtgggtttg
    32581 agtatggttg cgaagggcat tttcctgtat gccggatctg atcgttggcg gttacggatc
    32641 gtcaaatggt gcttagagcg ggagctgatc gattatcgca tctggggctt ggcgttatgt
    32701 accctttctg tcttgctctt agatgcgttg ggctggggca aaggacccta ggttgttgaa
    32761 atgataagag caatctgatg cggctatgcc aaaagcatca tgatattgag ggcatatgaa
    32821 agatattgat cgagaaaagc tgtacgatac ctggcagatt catcaatcta tggattggcc
    32881 ggacgggttg gggggtgatg aagggcaact catgacgttg gatacagtca ttggaggatg
    32941 tttgacctat taccttgatg aacatcatct tgatgaagca cggattgaaa tcttacgtga
    33001 ttgtcttgct gagctggaat ctctcgtccc tgacctaact gaagccacga gtgaatattt
    33061 cagacgtttg caatttcttg gcgtgaccct tctacaagat ttttcttaaa tctaattttg
    33121 ctttttttcg ctcttcctgc cccttttccc gttgcaacct aaatgagctc cctttttgga
    33181 caatttagga cttctgactt tcatggaaat ggttttggct ggtttgacgc tggaaaaaat
    33241 gctgtggtac cgtggttgct ggtttctacc tattgcctgt cacagtaagg taccgacaat
    33301 gagtcaacaa aatgacctaa aaacgatttt gaataaactt cattattccg acgatgccaa
    33361 ggtggtggcg cagatttcgg cccagaccaa actggtcatg gcacgaatga aggggattcg
    33421 ccgcaaggtg gtggtgatga gcggaaaagg cggggtgggg aaaagtatga ccaccgtgaa
    33481 tctggctttg gccttcgcgc gaatggggca acgggtggga ttactggatg tggatattaa
    33541 tgggccttgt gtgccgcaaa tgttgggcat gcgcgggaag ggcttattgg ataccccgga
    33601 aggggcggtg ccgccgaccg gtccgttaaa tattaaagtg gcgtcgatgg attttctcct
    33661 acaagaaaac gccccggtgc ggtggaaagg tcccatggac ctcagcccgg tttggttggg
    33721 catgaccgaa atgaatgtga ttcgggaatt tctctccgac atggtctggg gagaattgga
    33781 ctatttgttg gccgacctgc ccccaggggc ggcagccgat aagccacccc tcctggccgg
    33841 actcattcct gatttagcgg gggcggtggt ggtgacgacg ccttccgagg tggcttcgaa
    33901 tgtcgtccaa aaatccattg cctatgccca ggagctgggt attgagattt tgggggtggt
    33961 cgagaatatg agccagtacc gctgtccctc ctgcggggaa gaaagtccct tgtttgaggg
    34021 ggataccgat tccatgtgtg aggccttggg tttgcctctc ttaggacgca ttccctttga
    34081 tcggaatttt gcccgcacct ttgataaggg ggaaccgatc ctggatggag gcaatcccac
    34141 tgcggcgaaa tatcaggaca ttgccaaaaa ggttcataca ttgttggatt atcaacatgt
    34201 tttagacggg aaagtgtaac gtctcgaagc gatcgttggc cttcgccgac gttcagaagg
    34261 gaaacgatat gaagtttgtt tgtctcaaat gcgaaaccta tatgactttt gaaaaagtcg
    34321 aaaaacctgc tgaaggctca ttgggggtgt tttttgaatg cccttcctgc caatcgcggt
    34381 tttcgatggt gaccaatccc ggcgagaccc agatggtctc ctctttaggc gttcagttgg
    34441 gtgggagaac cgaagcgccc aaaccgttag aaatgactcg gggcgggtta gaggataatg
    34501 tttcggctgg catgggacag atggccgcct atttgaatga gaaaattcaa ggtggccaac
    34561 cggccgcggc tccagcggcc tcggctgcgc cggctactgc cagtgcacca ggtacaactt
    34621 cggaaggcgg cggatgtccc ttttcggcaa tggtcgccca aatgggtctg ggctcaacgg
    34681 gcgccacagg caccccagcc cctccggccg gggagcaatt gatttggtcg ccggatgccc
    34741 aagagaagct ggccaaactg ccatcgtttg tgcagccgat ggtcaaaagc agcgtagaga
    34801 cctatgcccg gaagagcggt tttaccaccg tgactcttca ggtgatggat gattccaaga
    34861 acgcctccac ggaagggata aagtggacgc cggaagcgca acaacggttg gataatattc
    34921 ccgatttcat cagacccatg gcacggcggg aaatcgaacg tcttgtcaag gagcggggtc
    34981 agtcggaaat tacggcagag atcatggaag aggccaaaga gaagtttatg aagtttatgt
    35041 agtctcgata gatatatgca ggttttagga aaggcggctc ttacacaaga gccgcctttt
    35101 ttgttttgtt tattgcagaa agcgggggct aaatcccgca aatcctttaa ggaaaatcct
    35161 tccaattcgc gtatggcttt gttcggtaaa tggcttctgc cttcacg
//
LOCUS       DAORAQ010000016        34947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_260_length_34947_cov_66.371375, whole genome shotgun sequence.
ACCESSION   DAORAQ010000016 DAORAQ010000000
VERSION     DAORAQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 34947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34947
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_34947_cov_66.371375"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKM72_02960"
     CDS             complement(<1..428)
                     /locus_tag="PKM72_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNP59771.1"
                     /translation="MSKGWQLTLILCLLGLFALFYQGLWGDPRHIPTVLIETDAPNFE
                     GPDVETGQTISLEQFKGKVVLLNFWASWCYECKVEHESILQLHQLFKDNPEFVMLGVN
                     YQDELPDAQKYLKEYGSTFSHVRDLKGQLAIDYGVYGVPET"
     gene            complement(425..2374)
                     /locus_tag="PKM72_02965"
     CDS             complement(425..2374)
                     /locus_tag="PKM72_02965"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNP59772.1"
                     /translation="MIIEIGHFSTIVALVLSVFGMASAALALKTRNPDWARSGRMAIT
                     LIFVLLGVGMLSLVYAFIIRDFSVLYVANNSNSKLPFFYTVAAVWGGHEGSLLLWVFI
                     LSVFSTLAVWLHWRTQPTIMPYLIAVESAIIFGFLCLIVFLSSPFERLLPVPLDGKDL
                     NPLLQDPAMVMHPPMLYMGYVGFSIPFSFAMAALFSGRLGEEWIKVTQRWSMFAWMCL
                     TTGILLGGYWAYYELGWGGYWGWDPVENASFMPWLVGTAYLHSVLVQEKRKMFKVWNL
                     FLIIVTFSLSLIGTFLVRSGVLTSVHSFATDPERGLYILIFVGTVIGIAFGALILRSN
                     KLKSQVEMDSLVSRESIFLFNNLFFLVAAATVFLGTLYPLILETLQDAKVTVGPPYYN
                     AVFMPIALGLLFLMGIGPYIPWRKASVASLKKSFSTPLLAGAVIVVLLFVTGIHNLYA
                     LAGGYVVTFAGMAIVLDFGKISQLYAQRNGSNIFQGGLAAFTANQRRYASMLTHIGVL
                     VLVVGIIASTVYQTEKVVSMRVGDEFAIGDYRMKLAKIHEVAGGNWNAQEGVFQVFKG
                     EDLITELRPQKRIYSATQTPTTESAIYATNMGHVFLTMPEVSEDGVAVARGVLNPLVL
                     WVWGGGVIMGLGVILNILRPRKKEE"
     gene            complement(2393..2848)
                     /gene="ccmE"
                     /locus_tag="PKM72_02970"
     CDS             complement(2393..2848)
                     /gene="ccmE"
                     /locus_tag="PKM72_02970"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNP59773.1"
                     /translation="MGTKNKKIAIIVSILLVAGSLGYLAFGNFGENIVYFVTPSEVKA
                     FTAEAYSKKVRVGGMVVKGSLKTQPEPVGLTFELTDGASTIPVAFQGIPPDLFKEGQG
                     AVVEGQWRDGRTFHSTMIMAKHSEDYMPMELKQAGVELPKKDFMKTLSP"
     gene            complement(2832..3344)
                     /locus_tag="PKM72_02975"
     CDS             complement(2832..3344)
                     /locus_tag="PKM72_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNP59774.1"
                     /translation="MTGLYTRWALILGATLLLAILTYQHYQQHLSTLSMGTLLSSPPV
                     TQPVRIQGMVKSGTLQGDVEHGQATFEFVDGETSLAVEYQGPPLENIRELKTLVLIGR
                     WDSQAQIFRAQDTALINNFGFVAAAYLISVLALGWMVFAMSQRVMVLFKEIKESKLYE
                     PEADSLGYKE"
     gene            complement(3341..4066)
                     /gene="ccmC"
                     /locus_tag="PKM72_02980"
     CDS             complement(3341..4066)
                     /gene="ccmC"
                     /locus_tag="PKM72_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HNP59775.1"
                     /translation="MINRIIRQIQRYKGWFGGGAALCIFLGLYIGLLASPPDYYQGEL
                     VRIMYVHVPLAHTSTLAYSVLFFGSIWYLWKRDPLVDNMCQASASICALFTSLALVTG
                     SIWAKPTWNTWWTWDPRLVSFTVLLLILGGYIMLRRLVDDRVQGGRYAAILAIVGGID
                     LPIIKFSVEWWRTLHQPMSFSTRGVSISEDILVPLTLMSIGCWLLFAYMVMVRTQILY
                     LTDLLHAKKGRFLSQTHFSQTTP"
     gene            complement(4141..4818)
                     /locus_tag="PKM72_02985"
     CDS             complement(4141..4818)
                     /locus_tag="PKM72_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNP59776.1"
                     /translation="MPFFNTIRWVVWKDLISEWRTRETMSSMLFFALIVILVFSFSFS
                     MDQDAARQLIAGIIWVAFTFTGIIGLGKSFTSELQNDCLESLQMCPAPKGAIYLGKVA
                     ANFLFMLSVEILLFPLFVLFFNLDVIEAVGTLLIIFFLATLGLSAVGTLFSALTVQIR
                     AREVMLPVLLLPLAVPVMIAAVEATRGALSGDPFSFYSQWIQLLIIFDIIFTVVSFWL
                     FEFILDS"
     gene            complement(4805..5479)
                     /gene="ccmA"
                     /locus_tag="PKM72_02990"
     CDS             complement(4805..5479)
                     /gene="ccmA"
                     /locus_tag="PKM72_02990"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNP59777.1"
                     /translation="MIAIQVSNLSKAYGFYRIFEDLSFDIHPGECFALFGPNGAGKTT
                     LLRILATLQAPSHGQFTIFGQDGVAQKDTVREIIMLIAHGSHLYDELSATENLKFALG
                     MRGQAPSPPQIKRALDRTGIGAFADLKIRQYSAGMKKRLDFAKTILAQPQLLLLDEPY
                     NALDHDGVAITNQLIQETLDRAGTVFMTTHDRDKATQIATRGGILKGGHLQLLSPEQL
                     TTDAIF"
     gene            5905..6525
                     /locus_tag="PKM72_02995"
     CDS             5905..6525
                     /locus_tag="PKM72_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNP59778.1"
                     /translation="MKSILGLRATYHYLSKSWPFLGGIGIFCALLGCDSGYSESTYAD
                     APSTHTVASRPSKGSPAPDFRLMDMHGKAVSLSDFQGKVVLLNFWATWCGPCRVEMPA
                     MEALYRSMQSKGLEIVAVSVDQQGTAVTRPFQEAMGLSFPILHDQDYEVGLTYGARTL
                     PMTFAIDRQGIIRQVVFGSRDWNSPEARRGIAEVLQEPVPESSQRM"
     gene            6533..7282
                     /locus_tag="PKM72_03000"
     CDS             6533..7282
                     /locus_tag="PKM72_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNP59779.1"
                     /translation="MIDSISQVSLLAAFSAGLLSFVSPCVLPLVPSYLSYITGLSVEN
                     LAKVEERERFKSAIILNALLFIAGFSTVFIAFGASASLMGQWLYDYQDVIRKVGGVLI
                     IIFGLYLLGILKLNFFMTERRLMHFETRPVGYLGSFLIGTAFAAGWTPCVGPVLGAIL
                     AYASTTESMSSGVMLLSAYSLGLGLPFFLTAFGMDTFLSYFKNFRSYLGGVSFVSGGL
                     LVAVGVMIYADSLTLITSFLERNGIGWYIGQ"
     gene            7743..8762
                     /gene="galE"
                     /locus_tag="PKM72_03005"
     CDS             7743..8762
                     /gene="galE"
                     /locus_tag="PKM72_03005"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNP59780.1"
                     /translation="MILVTGGAGYIGSHTCVELIRAGCDVTVFDNFSNSHAESLARVQ
                     RITGKSLRVICGDCRDRAAVVTALRESRATAVIHFAGLKAVGESVQQPLAYYDNNVVG
                     SLRLLEAMGECGVKQLVFSSSATVYGDPQRLPLTEDHPLSATNPYGRTKLMVEEILRD
                     VHRSDSSWRIGILRYFNPVGAHDSGLIGEDPQGMPNNLLPFVAQVAVGRREYLNVWGD
                     DYATPDGTGVRDYIHVVDLALGHLKALEALARLDVPKECLTVNLGTGNGYSVLEIVRE
                     FEAASGKPIPYKVAPRRSGDIASCYADPNQAFTLLGWRAERGLSEMCADAWRWQRDNP
                     QGYAG"
     gene            complement(9231..10283)
                     /gene="bamD"
                     /locus_tag="PKM72_03010"
     CDS             complement(9231..10283)
                     /gene="bamD"
                     /locus_tag="PKM72_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNP59781.1"
                     /translation="MTFPVNFRILSTTFLAALVMIAGCSSTPKPTDTTSKQAVSSTDE
                     QIFVGDSVEMNYDPNVIMKRAESFFDKESYAESIVEYKHFLDLHRNHVLAPYAQYKIG
                     VSHFKQYRTVDRDPEPLDLAIQNFDKLLQEFPASRYETEAKQTILICKEQLAQRHLMV
                     GQFYLNRDSYLAAAHRFEKIIKEFPELETAGDAMFHLAKTYQDLGIEEWSQEWLLALV
                     NEHPNNSYHSDGQKLLAKLQKKNPTFLASLPHDPSMDEQTDPNPLTIEQKPENQTLVR
                     TVSHSPESPLLSSVGTPSPALTPTTLSDSIASKPPVSDGACTIGTWCDSPSSTITTPP
                     TQLSSNVKSCKTGEWC"
     gene            10519..11511
                     /locus_tag="PKM72_03015"
     CDS             10519..11511
                     /locus_tag="PKM72_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNP59782.1"
                     /translation="MNGKLSRIVGTGSFLPSRRVDNQEVADLLGIEPSYIFRVSGIRT
                     RFWAGPQEECSFLAEQATRRALDQAGLVPENLDAILVSSTSPEMIFPSTACLLQARLG
                     IKGIPAFDLSASCSGFLYGLSMADCFIRAGQFRRCLVVASEIKSRSLDVKDLSTTILF
                     GDGAGAAIVEKSSNADMGVVSIRLHADGAYHDLVKIAGGGSRQPLTQSVLGSNEHTMR
                     IRGSRLFRIAIKRLGQAVRNHLEQEKWDVSELDQVIFHQANGRMLAQFCENIGIPHER
                     CLTMIEQVGNTSSASLPMALDHANRGQRLKKGDQVMLGAFGGGLTWGTALVRWG"
     gene            11669..12607
                     /gene="ispH"
                     /locus_tag="PKM72_03020"
                     /gene_synonym="lytB"
     CDS             11669..12607
                     /gene="ispH"
                     /locus_tag="PKM72_03020"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNP59783.1"
                     /translation="MKIFLANPRGFCAGVDRAIEIVDLSLKTYGAPIFVRHEIVHSRH
                     VVESLRGKGAIFVEELNEVPDGAIVIFSAHGVAKEVWEESTRRNLKVIDATCPLVIKV
                     HNEVNRDYSNEYELILIGHAGHPEVVGTLGQVPDKFHLVSSVEDVDSLEVENPLHLSY
                     VTQTTLSVDECRDIVAALHRRFPGIKGPHQEDICYATQNRQNAVKELSRFVDVILVIG
                     SPNSSNSNRLRELGERCGIPSYLIDSYQDIQTQWLEGVNAIGIAAGASAPEVLVAQVI
                     KFLKEQGATSVEELTVVEENVEFLLPKELLMAKVPR"
     gene            12932..16609
                     /gene="smc"
                     /locus_tag="PKM72_03025"
     CDS             12932..16609
                     /gene="smc"
                     /locus_tag="PKM72_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNP59784.1"
                     /translation="MYLRTLVVSGFKSFAEAKIDFPNGITAVVGPNGTGKSNIVDAIL
                     WAMGEQSVKSLRSERMEDVIFNGTEQRKPMGMVEASLIFSEVTPRELEPVSALLEGLD
                     QATDVMITRRLYREGDSEYYFNKIPCRLKDIRGFLWNARAGARGQSVIEQGNIEQLLS
                     ASPQERREFIEGTAGIIRYKKQKAEALRKLQSTENNLLRVRDILGEVRSQLRTLNRQA
                     KQAEEYQTFLREARELEVRLLTHDFRRFFQDQCQFENELHESENQELSCVAEEARLVA
                     EHQEVQLELTSSSEILKEAQDRFREIEQQMSNAYTAIQIERNRLDQYEQQHEQVMEER
                     ARLNGEGQDATGSLEALRARLAQIRCEMEILSVTVKDGEGAIADLAVRRRETAEKVDK
                     GRETILALAVDKTNQENRLRSIGEGQSSMARRIERLAVEYAQSQTDQTTLQSESEALR
                     RECSSLESQLDELRNNQDRLEVNLQSQRETREELDGKILDFQTQTAGAESELRAIQSV
                     FREEIGYGHHGEGDEASIRVACSCIQEALAERMGVPEDVEKAMEAVLGERLQAWIVQS
                     PQEAEMSIAQFKQHEWGKGSFIPLNIPRQGRGGPADWWSIVQHDTEVLGLAVDFVQVP
                     DELKPVVEALLGNTVIVKSLAGALNLMTRHSWFQGNGPLLVALDGELVSPSGVISGGS
                     GGEAGGLLRRRREILALEERLNTLSVGLEEAKANRKLLLQEIEDLSQQLEEATLSIRE
                     MEFQMLTIQKEASSKEKALPDLVRRLDALQQDRLAEEEEWGQLQVEEVEVRTRLERLN
                     QTRAQEDEALRQLLDSLNALDQERQDMLESLNDTRMNFQSLKSQWDHEQANVERIERE
                     EEHRNSRIRQIDGQLEHLFLQSRKSQEERLTNEDLVEELSIQKEAIAGTLLELGNRHA
                     ECMEGVKRLDGLIAKARETLSHIFKSRVPIEGRLAEVRAKFHAVQETLTMTYEISTDD
                     LKYSQDAEQTPEVLEGTEQASDEEKTGQWREQLQGIRKKLERIGPINLAAIEEHAQLE
                     ERFQFLLAQEEDLAGSIQSLQEIIQRLNQTTNKLFVDTFKELQVKFSEVFSALFAGGR
                     AELILVEETQEGQESEAPALEPGVEIVAQPPGKRLKNLNMLSGGEKTMTVMALLFASF
                     LIRPSPFCVLDEVDAPLDETNVVRFGQFLRQMADRSQFIVITHNKRTMETANSLFGVT
                     MEDPGVSKLIAVRLHELEEVS"
     gene            complement(16713..17060)
                     /locus_tag="PKM72_03030"
     CDS             complement(16713..17060)
                     /locus_tag="PKM72_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNP59785.1"
                     /translation="MSLLITEECINCGACLPECPNEAIFETRSAAEEKGHKVGEGQGD
                     GDTVYVITYERCTECVGHFDEPQCAAVCPVDDCCIPDPEIPETTDTLLDKAKELNPDK
                     EIDPAKVWAGVRN"
     gene            17641..18537
                     /locus_tag="PKM72_03035"
     CDS             17641..18537
                     /locus_tag="PKM72_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNP59786.1"
                     /translation="MFSAYVALTTAAMVWGGSVVAQKVALGPFSPVEASVFRGLGALL
                     ILIPLWLWKEGIVSFSRRDWRNFFLLGLGVLGNHLFVLFGLQFIGAGAAGIIIGASPA
                     ITAFLSSLILKDVPLRVIWFGCLVSFFGVLSISGREAVEGLGTNPWLGGSLVVCALVS
                     WALYTIGCRKTMERFSPLTVTWTTLLISLIFEIPLLAMNHKVLGAGLATVPVSGWLAL
                     LYVMVFATALGQQAWLYGVKGIGPSRAGIFGNLIPVSALFFSLVILGEPVGMREISGI
                     GLILLGVWLVDRQSCAVLVKVT"
     gene            18575..18967
                     /locus_tag="PKM72_03040"
     CDS             18575..18967
                     /locus_tag="PKM72_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNP59787.1"
                     /translation="MLKERIEEVTQMNELFYRGFEQLDVALMDSIWAHQEYVTCIHPG
                     WSIRVGWPAVRDSWVVIFNNTFSMKFELTEVQVQVAADVAWVICTENITSRVGENEQN
                     SQVVSTNLFERIGDEWKIIHHHGSPLME"
     gene            complement(19114..19335)
                     /locus_tag="PKM72_03045"
     CDS             complement(19114..19335)
                     /locus_tag="PKM72_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59788.1"
                     /translation="MNADTCLIGGCTEPLYAPAGTQSLCKDHFLHFVTWRRKKGGLGM
                     FKKYCALNMEERDVIVEEWAKSTFSSSSS"
     gene            19574..20050
                     /locus_tag="PKM72_03050"
     CDS             19574..20050
                     /locus_tag="PKM72_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59789.1"
                     /translation="MLTATDVFEQTREMALRATSQEERALQIDYPALGKKIEQALTGR
                     KINLLYVNEFLPEGYEDQGRFNVMVMTAGNVLFDMVIGDSYFRYDIFSCCDLDKIQLI
                     DGTWDNKEKRTEESFLSLRLMHGDEAHILLALKDEQRPAVLSFAEKISHSRHPEKV"
     gene            complement(20098..21192)
                     /locus_tag="PKM72_03055"
     CDS             complement(20098..21192)
                     /locus_tag="PKM72_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HNP59790.1"
                     /translation="MKTLALHKQHESLGARFQPCGEWEVPLHYGNPLLEYESIHQRGG
                     LADLSLRGKIMVTGDDRVSWLQSIISNDILPLKPGQGRYSAFMNHKGKMLSYFRVFLQ
                     PETLIIEDVGEVDDLTFQALRKFLLYGTKAKLHNGLESWGLLLVTGPNGPDILKQALG
                     VEVNSLQVLDTISFTFGDMQGVIARTEETGGQDFELFVQAEGLPALWSHLLTIGKEAG
                     LQPVGREALETARIEGGLPRLGPDLNEKIVPPEANLEGIAFSLSKGCYPGQEVVARMD
                     TYGSVKRRMVGLVLEVEPPQLPEAGAKLFSGTREVGWVSSSTHSPRIKRPIALGFPLR
                     DFTQPQTKLEIEIRDQRFPASVCALPFTAL"
     gene            complement(21189..21572)
                     /locus_tag="PKM72_03060"
     CDS             complement(21189..21572)
                     /locus_tag="PKM72_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNP59791.1"
                     /translation="MSDTKTILVAVSDIFFYTKIRDAFLPQGYKLERIRTGDDWQTKA
                     LASQPMGIIVNMNDDRLNASDIVKSLRTLPQTQALPILAFANHEEVQTWKLAKDLGIQ
                     KIVSRNEFSARTLALFEEITTAATS"
     gene            complement(21594..22109)
                     /locus_tag="PKM72_03065"
     CDS             complement(21594..22109)
                     /locus_tag="PKM72_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP59792.1"
                     /translation="MDIQDFLDQGEGHEEDKVTAWNLFQQAYELQMKGQLEEAVDLYK
                     QSIDIFPTAEAHTFLGWAYSFMGQLQEAIEECQVAIRQDPEFGNPYNDIGAYLIELNQ
                     LDEAIPWLEKAMKAKRYENPSFPHMNLGRVYERQGEWDQAVDSYKKSLALNPEYKTAK
                     QALMRILTLMN"
     gene            complement(22162..22962)
                     /locus_tag="PKM72_03070"
     CDS             complement(22162..22962)
                     /locus_tag="PKM72_03070"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNP59793.1"
                     /translation="MNPINIAPLRERDITRHIAREYYKEFDSLIESDIIIVGGGPSGL
                     LCARDLAATGFRTLLIEQSLALGGGFWSGGFLMNKATICEPADRILEELGIPFKPIKD
                     CPGMTMVDPPHVTSRLISAAYEAGVKIMNLTKVVDLILRQDQRIEGVVVNNSTVEMAG
                     HGTIHVDPIALESQIVVDATGHDAVVVNLLHQRNLYQKVPGNGAMWVARSEVLVVENT
                     REIYPNCFVAGLAVAAVDGSPRMGPAFGSMLLSGRRAAELVQSKLKGE"
     gene            complement(23082..24971)
                     /gene="thiC"
                     /locus_tag="PKM72_03075"
     CDS             complement(23082..24971)
                     /gene="thiC"
                     /locus_tag="PKM72_03075"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNP59794.1"
                     /translation="MRVVSEEPRIPLSSLSIQPFPGSQKIYRTGSRPDVRVPMREIHQ
                     TPSHSPQDHTEVQNPSVIVYDTSGPYTDPALHIDVRKGLHPLRKDWIAERQDVEPLKE
                     VSSQYGRTRANDPALQAIRFHLQRPPLRAKSGRNVTQLHYARKGIVTPEMEFIAIREN
                     QAREEHQSAQHGTGQTFGVTQHPGQNWGASTPSYITPEFVRDEIARGRAIIPANINHP
                     EIEPMIIGRNFLVKINANIGNSAVSSSIEEEVEKLIWSIRWGGDTVMDLSTGKNIHET
                     REWIIRNSPVPIGTVPIYQALEKVGGKPEELTWEIFRDTLVEQAEQGVDYFTIHAGVR
                     LAYVPLTASRMTGIVSRGGSIHAKWCLAHHQENFAYTHFEEICEIMKSYDVSFSLGDG
                     LRPGSLADANDAAQFAELETLGELTHIAWKHDVQVMIEGPGHVPMQLIKENMDKQLSV
                     CDEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGCAMLCYVTPKEHLGLPDKEDVK
                     AGVMAYKIAAHAADLAKGHPGAQHRDNALSQARFEFRWQDQFNLSLDPETAKDFHDAT
                     LPAQGAKLAHFCSMCGPHFCSMKITQDVREYAAEKQLADEAALKQGMQEKSEEFRKTG
                     GDLYL"
     regulatory      complement(25119..25221)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(25289..26494)
                     /locus_tag="PKM72_03080"
     CDS             complement(25289..26494)
                     /locus_tag="PKM72_03080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP59795.1"
                     /translation="MNKKIKDDTPVRVLHLIDRITGYGTTRLLWDIVRLTPPDKVKHL
                     VISFSPDKGKWITADLLREKGAYGQVPNRRILKLLDRWSLTWFLGRYISTIWHVCKAL
                     IWFRPDIINVHTTYALTIGVPLKVLLRRPVVHLVPSLFSQMVDEGKPWVPRLYARFHG
                     LFDCFFTAASRCQDELHAVGIPNSKIVPLRGILDLEEINKIRHKREQFHKTIRENYHL
                     PSDAFIALSVGRLDSSKGHIYALEALSSLVQQFPKLYWLLVGDGKQRAELENRAKDLG
                     TYTHMHLVGYQDDLLPYYAAATMFLRTMIFEETNLSTYKAMAMGLPIIGFDTGCETEL
                     LKTVGHGILVPNKSVAGLSAAVAQILTLPDHGRELGGRGIEFCQANLDIRLGIEDITA
                     VYKKLRDGG"
     gene            complement(26742..27911)
                     /locus_tag="PKM72_03085"
     CDS             complement(26742..27911)
                     /locus_tag="PKM72_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59796.1"
                     /translation="MPLEAGVLVMSKQKPSTKKSIFFHIVLLLIPITTLVLTYLAYTT
                     FRTVQFYNYVKSDQRGWSGQVHQAHKELGYAPIPNSHGWEIMPLGPDIPVRYDKDGFR
                     IPVDEKLKTTNNHPYALTLGCSFTYGAATHAENTFPYLIGADFGVSTKNAGVCSYGLS
                     QMVLIAKLLIPRHKPDYVIVQYSPWLVKRAIQPFAPSYYGKLPNPYYFGESALGIAPA
                     VFQTKVFDLPIDNYRNTQKGVSDFFLFSVNIGFPLLLHDDFNMTKYTLAKFLRLTNGP
                     TKNRSAVIQDAYKEIANMAEQNNARMIIVVLGENHLPVPISKDLFPRNAVVIDAHSEL
                     LNRLPTIDEKSFQRHYNHWRGEPPTLVDTHPNELAHKIIAEMITLQMVKFSKKEI"
     gene            complement(28134..28871)
                     /locus_tag="PKM72_03090"
     CDS             complement(28134..28871)
                     /locus_tag="PKM72_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP59797.1"
                     /translation="MPLPWDSQHFEISVGQIISPALTNSELENILSFAKEQGYHLIYW
                     ATCPDRMIPLPLLQSFSGSLVDRKVTYQRKLSTEFTFGEIKNPSHSFEVIEFPRSQAN
                     QQLKTLALIAGGHSRFHDDPCISKEKFAGMYHIWINRSTLHEIADVVFIVKNSANMED
                     CLGMVTGSVKNGIGKVGLMGVQKKVQGQGIGSLLMKTLHEWLISNGIHTSEVVTQKNN
                     IRACKFYERFGYKLESLQHYYHFWVQL"
     gene            complement(28951..30147)
                     /gene="rffA"
                     /locus_tag="PKM72_03095"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(28951..30147)
                     /gene="rffA"
                     /locus_tag="PKM72_03095"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNP59798.1"
                     /translation="MEKIIPFNKPFIAGKELYYIAQAVTFGNLAGDGYFTKKCSELFE
                     KQFGVHKVLMTPSCTAALEMAAMLCNLGPGDEVILPSYTFVSTANAIVRLGAKPVFVD
                     IRPDTLNLDENRVEEAITPKTKAILPVHYAGIACEMDHIMAIAKKHALLVIEDAAQGV
                     GAYYKNRALGTIGHLGTYSFHETKNYICGEGGALCINDPGMIRRAEIIRDKGTNRSEF
                     FRGEVDKYTWVDIGSSYVPSEIVCAFLYAQLEMLDSIAERRRGIFKRYRMLLRPLEIQ
                     GLLSLPTIPGDCKSNFHMFYILLPDIETRDALMGYLRQQNILAVFHFVPLHSSPMGQK
                     FGYIEGDFPITEQLSGRLLRLPFYYEMTEKEQEYIIKCITEGLETVKGKIKLNREKPP
                     ALRFVQ"
     gene            31837..32934
                     /gene="nadA"
                     /locus_tag="PKM72_03100"
     CDS             31837..32934
                     /gene="nadA"
                     /locus_tag="PKM72_03100"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNP59799.1"
                     /translation="MPTITEFPITDYQTLPDQELFDRIVDAKAILGRKVLLLGHNYQR
                     DDVIIHADFRGDSLLLAKYAAEHPEFPHVVFCGVHFMAETADILSRSNQTVILPDMAA
                     GCSMADMASMEQVDDCWERLARVLPVEETVTPIVYVNSAASLKAFCGEHGGVTCTSSN
                     AKAIMNWAWERREKILFFPDEHLGRNTANAMGFPPEEMIVWDPFQPNGGHTRETIHKA
                     RLILWKGHCSVHQMFQPAHVSYFRKQYPNVQVIVHPECHEDVVNQADLVGSTEFIIRT
                     VTQADPGTIWAVGTELNLVNRLKHDQADKQVFFLSPMVCRCSTMYRIDGPHLCWAMEN
                     LVQGHVVNHIQVPDEEKVFAKLALDRMMDLS"
     gene            33113..>34947
                     /gene="ileS"
                     /locus_tag="PKM72_03105"
     CDS             33113..>34947
                     /gene="ileS"
                     /locus_tag="PKM72_03105"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNP59800.1"
                     /translation="MDYKATLNLPRTEFPMKANLPQKEPERLAWWEQERVYDRIQEAR
                     NGSPRYILHDGPPYANGHIHIGHGLNKILKDIIIKSKTMAGFQAPYIPGWDCHGLPIE
                     HQVTKQLGPKKKDLSAVELRKLCREYAEKFVEIQRDEFRRLGVFGDWEHPYLTMDHAY
                     EAAIVREFGKFVEKGRVYKGLKPVLWCTVDQTALAEAEVEYENHLSPSVYVKFPFTVL
                     PAEMGSDQRLGLPTVKKLKAVSAVIWTTTPWTLPANQAICLHPDFDYAVLQAGEEAFI
                     VALGLVDAFAKACKIEKFEVLGKKKGKEFEGWTCHPPFAKKEIPILNGDFVTLEQGTG
                     CVHIAPGHGMDDYLLVLKYNKPPYVDMLPDRKPLEILVPVNNAGRFTDDFPEFAGQPV
                     LEANPGIVEVLNARKLLVGEKRLEHTYPHCWRCKQPVIFRATHQWFVSMEEADLRQKA
                     LKEIDKVQWIPERGRDRIYGMILNRPDWCLSRQRMWGTPIPVFSCQSCSTPLTDSKVI
                     EHISVQMQESGADIWFSRSAQELLPQGTTCVQCGHGLFKKEHDILDVWFESGVSHAAV
                     LRPQGEGWYPADLYLEGSDQHRGWFHSSLLTSVTTDEQAPYRAVLT"
BASE COUNT         8661 a         7792 c         9160 g         9334 t
ORIGIN      
        1 gtttcgggta ccccatagac cccgtaatca atggccaatt gtcctttgag gtcacggaca
       61 tgggaaaacg ttgagccgta ttcttttaaa tatttttggg catccggtaa ttcgtcctga
      121 taattcaccc ccagcatcac gaattcggga ttatctttaa agagttgatg aagctgtaag
      181 atgctttcgt gctccacctt gcattcataa caccatgacg cccaaaaatt gagcaggacc
      241 actttcccct tgaattgttc caaagagatc gtttgtcccg tttccacatc cggtccttca
      301 aagttgggag catcggtttc aatcaggaca gtgggaatgt gtcgtggatc tccccagagc
      361 ccctggtaaa acaaggcgaa cagtcctaac aggcagagga tgagtgtcaa ttgccagcct
      421 ttactcattc ttccttcttt cgggggcgga ggatattcaa aatcacgccg agccccatga
      481 tgacaccgcc tccccatacc cacagaacca gtggattgag caccccacgc gcgacggcca
      541 ccccgtcttc ggacacctca ggcatggtca gaaagacgtg ccccatgttc gtcgcataaa
      601 tggccgattc cgtggtaggc gtttgagtgg ccgaatagat tcgcttttgg ggccggagct
      661 ccgtaatcaa atcctctccc ttaaatactt gaaagacgcc ttcctgggca ttccaattcc
      721 cgccggccac ttcatgaatt ttcgccagtt tcatccgata gtccccgatc gcgaattcgt
      781 ctcccacgcg catcgagacg actttttccg tttgatacac ggtggaggca atgatgccga
      841 cgactaggac gaggaccccg atatgggtga gcatgctggc atatcgtcgt tggttggcgg
      901 taaaggccgc tagaccgccc tggaagatat tactcccgtt ccgctgcgca tacagttgcg
      961 aaatttttcc aaagtccagg acgattgcca tgccggcgaa ggtcaccaca taccctcctg
     1021 ccagggcata aaggttatga atgccggtca caaatagcag gacgacaatg accgctcctg
     1081 cgagaagggg tgtggagaaa ctttttttca ggctggccac cgacgctttg cgccagggga
     1141 tatagggacc gattcccatg agaaataaca atcccagggc aatgggcata aacaccgcat
     1201 tgtaataggg gggacccacg gtcaccttgg cgtcctgcag tgtttcgagg attaatggat
     1261 agagggtgcc cagaaagacc gtggcggcag ccaccagaaa gaatagatta ttgaagagga
     1321 agatcgattc acgggaaacc aacgaatcca tctcgacttg tgatttcaat ttattggatc
     1381 tcaggattaa tgcgccgaag gcgatcccga tgacggtgcc gacgaagatg agaatataca
     1441 gaccgcgctc aggatccgtg gcaaaggaat ggaccgaagt gagcacgccg ctccgcacaa
     1501 gaaaggtccc gatcagagac aaagaaaagg tgacgatgat gaggaagaga ttccagactt
     1561 tgaacatctt ccgcttttcc tgcaccagaa cggaatgcag gtaggcggta cccaccagcc
     1621 agggcataaa cgacgcgttt tcgaccggat cccagcccca atagcctccc caacccagtt
     1681 cgtaataggc ccaatagccg ccgagcagga tgccggttgt caggcacatc caggcaaaca
     1741 tgctccatcg ctgtgttacc ttgatccatt cttctcctaa ccgtccggaa aataatgcgg
     1801 ccatggcaaa ggaaaaggga atggaaaatc ccacatagcc catgtagagc atgggtgggt
     1861 gcatgaccat cgcggggtct tgcagtaacg gattcaggtc tttgccatcg agaggaacag
     1921 gtagaagccg ttcaaaagga ctggagagaa aaacaattaa acataaaaaa ccgaagatga
     1981 tggccgattc cacggcaatc aaatacggca taatggtggg ctgagtgcgc caatgcagcc
     2041 agacggccaa ggtgctgaag acggaaagta tgaagaccca gagcaggagc gagccttcat
     2101 gtccgcccca caccgccgcg accgtataga aaaacgggag tttcgaattt gaattgttgg
     2161 cgacatagag aaccgagaag tcccggatga taaaagcata gaccaatgag agcataccca
     2221 cacccagcaa gacaaagatg agggtgatag ccatgcgccc ggaacgggcc caatcgggat
     2281 tgcgtgtttt gagggccagc gcggcgctgg ccatgccaaa gactgacagc accagggcaa
     2341 caatggtcga aaaatgtcca atttcaataa tcatagagaa tggcgaacag ggttagggag
     2401 agagggtttt catgaaatct tttttgggaa gttccacacc tgcttgtttg agttccatgg
     2461 gcatgtagtc ctcggaatgc ttggccatga tcatcgtgga atgaaacgtc cggccgtccc
     2521 gccattggcc ttccaccacc gccccttgtc cctccttaaa caggtccggg ggaataccct
     2581 gaaatgccac aggaatcgtc gaggcaccat ctgtgagctc aaaggtcagc cctaccggct
     2641 cgggttgggt tttgagactg cctttgacca ccatgccacc cacacgaact tttttgctgt
     2701 aggcttcggc ggtaaaggct tttacctcag agggagtcac gaagtacaca atattttccc
     2761 cgaaattccc aaacgcgaga tagccgagag aaccggcaac caggagtatg ctgacaataa
     2821 tggcgatttt tttattcttt gtacccaagg gagtctgcct ctggttcgta aagtttggat
     2881 tctttgatct ctttaaataa aaccatgact ctttggctca tggcaaatac catccacccc
     2941 aaggccagga cggaaatcag gtaggccgcg gccacaaatc caaaattatt aattaacgcg
     3001 gtgtcctgtg cgcgaaaaat ctgtgcctgg ctgtcccagc gcccgatgag aaccagggtt
     3061 ttcaattcgc gaatgttttc caggggagga ccttggtatt ccactgcaag actggtttcc
     3121 ccatccacaa attcaaatgt ggcctgccca tgttctacgt caccctgcaa ggtaccactt
     3181 ttgaccatcc cttgtatcct gaccggttgg gtaaccggag gactgctcaa aagcgttccc
     3241 atggacaatg tggataagtg ttgctggtag tgctgatagg tcaggatggc gagaaggagg
     3301 gttgccccca gaattaacgc ccagcgtgtg tataacccgg tcatggagtc gtctgagaaa
     3361 aatgggtctg actgagaaag cgaccttttt tggcatgcaa taagtcagtc aagtacaaaa
     3421 tttgagtgcg gaccattacc atataggcaa acaataacca acagccaatg ctcattaacg
     3481 tcagggggac cagaatgtct tctgaaatgc tgactcctct ggtggagaac gacatgggtt
     3541 ggtggagtgt tctccaccat tccaccgaaa attttatgat gggcagatcg atgcctccga
     3601 caatagccag aattgcggca tagcggccgc cctgaacccg gtcatccacg aggcgccgaa
     3661 gcatgatgta cccccctaaa atcaggagaa gcaccgtaaa cgagacgagg cgtgggtccc
     3721 aggtccacca ggtgttccat gtgggcttgg cccagatcga accggtgacg agggccagtg
     3781 aggtgaagag ggcacaaatg ctcgccgagg cctggcacat attatccaca agcggatccc
     3841 gcttccacaa ataccagatg ctcccgaaaa ataatacgga ataggccagc gtgcttgtgt
     3901 gagccagggg cacatggaca tacatgatcc ggaccagttc cccttgataa taatcaggcg
     3961 gtgaggcgag caggccgata tacaagccta aaaaaatgca gagagcagcc cctccgccaa
     4021 accacccttt atagcgttga atctgacgaa ttatgcgatt gatcatgtgc tgtattgtaa
     4081 agagagattc ctcaggggga aaggtccgta ggaactagac ttttaagccg ttttcaacat
     4141 ttacgagtcg aggatgaatt caaatagcca gaatgagacg acggtgaaaa taatgtcaaa
     4201 gatgatcaga agttggatcc attgcgagta aaaggaaaat ggatctccac ttaacgcccc
     4261 tcgcgtggct tccacggccg cgatcatgac cggaactgcc aggggaagga gcaggacggg
     4321 gagcatgact tcccgggctc gaatctgaac ggttaaggct gaaaagagtg ttccgacggc
     4381 ggataacccc aaggtggcca ggaaaaatat tatcaaaagg gttcctacgg cttcaatgac
     4441 atccagatta aagaataaga cgaacaaggg aaataagagg atttcgactg agagcatgaa
     4501 gaggaaattg gccgcgactt ttccgagata aatggcgccc ttgggagcgg ggcacatttg
     4561 cagggactcc agacaatcgt tttgcaattc cgaggtgaag gatttcccta agccgataat
     4621 gcccgtaaag gtaaaggcaa cccagatgat tcccgcaatc agttggcgtg ccgcatcctg
     4681 gtccatggaa aagctgaagc taaacaccag aatgacaatg agggcaaaga atagcatcga
     4741 cgacatggtt tcccgtgtcc gccattcact gatcaggtcc ttccagacga cccagcgaat
     4801 tgtgttaaaa aatggcatcg gtggtgagtt gctcagggga gagaagttga aggtgtccgc
     4861 ctttgagaat cccgccacgt gtagcgattt gggtggcctt gtcacggtca tgcgtggtca
     4921 tgaacacggt gccggcacgg tccagcgttt cctggatgag ctggttggtg atcgccaccc
     4981 cgtcatgatc cagggcgttg tagggctcat cgagtagcag cagttgtggc tgagccagaa
     5041 tggttttggc gaaatccaat cgttttttca tcccggccga atattgacgt attttgaggt
     5101 ccgcaaaggc cccgattcct gttcgatcca gtgcccgttt gatctgaggc ggggaagggg
     5161 cttgaccacg catgcccagg gcaaatttca gattttcggt ggcactgagt tcatcatata
     5221 aatgggaccc atgggcgatg agcatgatga tttctcgaac ggtatccttc tgtgccacgc
     5281 catcctgtcc aaaaatggta aactgaccat gagacggcgc ttggagggtg gctaaaatac
     5341 ggagtaacgt cgttttccct gccccgttgg ggccaaataa ggcaaagcac tctcccggat
     5401 gaatgtcaaa agacagatcc tcaaaaatac gatagaaacc gtaggctttg gagaggttgc
     5461 tgacctggat ggcgatcatg tcatggtatg agacttggaa aagttagcct tgcagtctac
     5521 gaaatgtatt ggaggttgtc taggaatgta acaagatgtt ggaaacagtc gctagcgatg
     5581 ggctttcatt attcatagat ttccaggtct cgcaggttat ggggtttcgt tgcgggatgc
     5641 ggccttgtcg ggtaaattga aagacatctt tggcaatccg attttcgtcg agaccctgtc
     5701 atatttcagg gcctcctcaa aaataggtaa ttggcagaca acaaatacca taaaactcaa
     5761 ataatcctgg tgggttgcct tatttagagt gagcatcgcg ttgacgatga aatttatcgg
     5821 gtcttatgat gacggttggt tgaaggagat ttccggtatc gtgaatcggg gattgcttga
     5881 atttttccgt ggggaggcag tgagttgaaa tcaatccttg ggttgagggc cacctaccat
     5941 tatctctcga aatcctggcc ttttttgggg gggataggaa tcttttgcgc tcttctaggc
     6001 tgcgattccg gctattctga aagtacctat gccgatgcac catctaccca tactgtagct
     6061 tcccgtcctt caaagggatc ccccgcgccg gatttccgtc tgatggatat gcatggaaag
     6121 gctgtctcgc tttccgattt tcagggaaaa gtggtgttgc tgaatttttg ggcgacctgg
     6181 tgtggaccgt gccgggttga aatgcccgcc atggaagcct tgtaccgcag tatgcaatca
     6241 aaagggttgg aaattgtcgc ggtctccgtt gaccaacagg gcacagccgt gacaaggcct
     6301 tttcaggaag cgatggggtt gagttttcct attttgcatg atcaggatta tgaggtgggg
     6361 ctgacctatg gcgcacgtac gctccccatg acctttgcga tcgatcgtca aggcattatc
     6421 cggcaggtgg tcttcggatc ccgggattgg aacagccctg aagctcgcag gggaattgcc
     6481 gaggtccttc aagagccggt tccagagtct tcccaacgga tgtaatacag ttatgatcga
     6541 ttcgattagc caggtgtctt tgcttgccgc atttagtgcc ggacttttgt cttttgtttc
     6601 tccgtgcgtc cttccgctcg ttccttctta tctctcctat atcaccgggc tctcagtgga
     6661 aaatctcgcg aaagttgaag agcgagagcg ttttaagtct gcgattattc tcaatgccct
     6721 gttgtttatt gccgggtttt ctacggtctt catcgcgttt ggggcatcgg ccagcctgat
     6781 ggggcaatgg ttgtatgact atcaagatgt gatccggaaa gtcgggggtg tcctgattat
     6841 tattttcgga ctatatttgc ttggaatttt gaaattaaac ttttttatga ccgaacgacg
     6901 tttgatgcat tttgagacgc gtcctgtcgg ctacctgggt tcatttctca tcgggacggc
     6961 gtttgctgcc ggctggacgc cttgcgtagg accggtgttg ggggccattc tggcctatgc
     7021 cagtacgacg gaatcgatgt ccagcggggt gatgctcctg tcggcctatt cgttgggttt
     7081 gggtctcccg ttttttctga cggcatttgg gatggatacg tttttgagct acttcaagaa
     7141 ctttcggtcc tatttaggcg gggtatcgtt tgttagcggc gggctgttgg tcgcggtcgg
     7201 cgtcatgatt tatgcggatt ctttgaccct catcaccagc tttcttgaac gcaacggtat
     7261 tggatggtac atcggccaat aaaccggcat gtacgacctg ggctatcctc ctctttcttc
     7321 tttcctcgtc tttccactcg cttgtatagt ttcgggaatg gtgggtggct taagcagatg
     7381 taggagtata ttggagcaac caatattcat ggaggagcga cggtcttctc ttggttatgg
     7441 agtggtgggg gccacaggaa aaattgaaca gaggattcat gcacggcttt tgttttttta
     7501 agccaccgcc tctcagtcat atatactgat agtcctcaat tcacctctat tccttaccga
     7561 tctatttctt gacgcacctt tcttctccgt gtagggtccc accacatttt ttgagactct
     7621 atattatgga atcgttggct tgtcagcatt gatgtgtctc tgcaaccttc aagtcatgta
     7681 aaaatggtca acatgaaaag aaaaagcagc gggctatctc aatttcaacg ggagcgtatt
     7741 caatgattct cgtaaccggc ggtgccggtt atatcgggtc acatacctgc gttgaactca
     7801 tccgtgccgg ctgtgatgtg acggtctttg acaatttctc caatagccac gctgagtccc
     7861 tggcgcgggt gcagcgaatt acagggaagt cccttcgcgt gatatgcggt gattgccgtg
     7921 atcgagctgc ggtggtgacg gctctgcgcg agagcagggc gaccgcagtt attcactttg
     7981 ctggcctcaa ggcggtggga gaatcagtcc aacaaccctt ggcctactat gacaataatg
     8041 tggtcggatc ccttcgcctg ttggaagcca tgggcgagtg cggcgtaaaa caactggtct
     8101 tcagctcttc tgccacggtg tatggcgatc cccagcgtct tccgctcacg gaggaccacc
     8161 cgctatctgc gaccaaccct tatggtcgaa cgaaactgat ggtggaggaa atcttgcgtg
     8221 acgtacatcg tagcgattcc tcctggcgga tcggcatcct gcggtatttt aatccggttg
     8281 gtgcacatga cagtggtctg atcggggaag atccccaggg tatgccgaat aatctcctgc
     8341 cctttgttgc gcaggttgca gtgggacggc gggaatactt gaacgtgtgg ggtgacgatt
     8401 atgcgactcc tgatgggacc ggagtgcggg attatatcca tgtggttgat ttagcattgg
     8461 ggcatctcaa agcgctggaa gcgctggctc gattggatgt cccgaaagag tgcttaacgg
     8521 ttaatcttgg tacgggcaac gggtacagcg tattggagat cgtgcgggaa tttgaagccg
     8581 cgagtggcaa gccgattccc tacaaggttg ccccacgtcg ttccggtgac attgcctctt
     8641 gctatgctga tccgaatcaa gctttcaccc ttctgggttg gcgtgcggaa cgcgggctca
     8701 gtgagatgtg cgcggatgcc tggcgatggc aacgtgacaa cccccaggga tacgcaggct
     8761 gacttttttc ccttggttat ccgggcacct ctgtgggggg gagccgttga cggcctggtg
     8821 ccagggaatt ccggtggatg cttgcttcat tgccgtgaaa cactctctcg gtgcggtcac
     8881 gtggtcggaa tcctcgcgtg gtcatatagg gcctcactgg aggcggcagt cccaccatgg
     8941 ttcctcaact taaccacatt ccacgttcaa tctaaaaaaa gcgggtccgt gaatttcgcg
     9001 gtagttctcg cggggttcgt ctttctgaga aagcatcacg aaagtggtca ggagcctgct
     9061 catggaacgt ttttgaaagg tgatggtctt cggtcaatcg attgtaagga ttaggccacc
     9121 caggtcaggt acttttggat ggcaaattgg tgttggtcgg agaatgtggc caacggagtc
     9181 aattggcctc tataagatta cgcgaaaaca aggttcaggg aggtcgtggg ttaacaccat
     9241 tcgccggttt tgcaggattt gacgttactc gataattggg taggtggggt cgtgatcgtg
     9301 gaggatggtg agtcacacca ggtcccaatt gtgcaggctc cgtcggagac aggcggcttt
     9361 gatgctattg agtcgctcag cgtggtcggt gtcaacgcag gtgaaggggt gccgactgag
     9421 gataacaatg gggattccgg gctatgggat accgtccgta cgagggtttg attctcaggt
     9481 ttttgctcta tggttaaagg gtttgggtcg gtttgttcat ccatcgaggg atcgtgggga
     9541 agggaagcta gaaaagtcgg attctttttt tgtagctttg ccaagagttt ttggccgtct
     9601 gagtgatagg aattgttagg atgttcattg accagggcaa gtaaccattc ctgtgaccat
     9661 tcttcgatac ccaggtcctg gtaggttttc gccagatgga acatggcatc cccggcagtt
     9721 tctaattcag gaaactcctt gataattttt tcaaatcgat gggcagcagc gagatatgaa
     9781 tcccggttga ggtagaattg cccaaccatc aaatgtcgtt gggctaattg ttctttgcaa
     9841 atcagaatgg tttgcttggc ttcggtttca tagcggcttg ccggaaattc ttgaagaagt
     9901 ttgtcgaagt tctgaatggc tagatccagg ggctcagggt cacgatcgac tgtccggtat
     9961 tgtttgaaat ggctgacccc aattttgtat tgggcataag gtgccaatac atgatttcgg
    10021 tggaggtcga ggaaatgttt gtattccacg atggattccg cataggattc cttgtcaaaa
    10081 aatgattcgg cccgtttcat gatcacgttc ggatcgtaat tcatttctac ggaatcacca
    10141 acgaaaattt gttcgtccgt tgaggacact gcttgtttcg atgtggtgtc ggttggtttg
    10201 ggtgtggagg aacatccagc gatcatgaca agagcagcga ggaaggtggt cgaaagaatc
    10261 cgaaaattga caggaaatgt cacaggtaag tcactccggg cagtagatga tcatgggctg
    10321 aggtgaatgt cgatacagag agtatgaaat agcctgtaat ctatacaaga ttcggaccaa
    10381 taaaagaaag tctttaatat tgcgattagg ttaaatcttt taccgaaaat cgtctctttt
    10441 ttgccacatt attgagtagg cataaaataa atgcgacagt cggtattatt gtgaatttaa
    10501 cgaataggcc acgagtacat gaatggcaaa ctctctagaa ttgtgggaac cggatccttt
    10561 ctccccagtc gtcgggtaga taaccaggag gtagcagatc tgctagggat tgagccttcg
    10621 tatatttttc gagtttcagg tatccggacc aggttttggg ccggtccaca ggaggaatgc
    10681 tctttcttgg cagagcaggc tactcgaagg gcattggatc aggccgggtt ggtcccggag
    10741 aatcttgacg ccattcttgt ctcctccacc tctccagaga tgattttccc ctccacggcc
    10801 tgcttgcttc aggctcgatt gggtatcaaa ggcatcccgg cctttgatct ctctgcctcc
    10861 tgctcgggat ttctctatgg cctttccatg gcggattgtt ttattcgggc cggacagttt
    10921 cgaagatgtc tcgtcgtggc ttcggaaatc aaatcccggt ctttggatgt caaagatttg
    10981 agtacaacca ttttattcgg tgacggggcc ggtgccgcca ttgtcgaaaa atcatcaaat
    11041 gctgacatgg gcgtcgtcag tattcgactt cacgccgacg gggcatatca tgatctggtc
    11101 aaaattgccg ggggggggtc ccgtcagcct ttgactcaat ctgtccttgg ttccaatgag
    11161 cataccatgc gaatacgggg ttccaggctg tttcgcattg caatcaagcg cttaggtcaa
    11221 gcggtccgca accatttgga gcaggagaaa tgggatgtct cggaactcga ccaagtcatt
    11281 ttccatcaag ccaatggacg gatgcttgcc cagttttgtg aaaatatcgg gatccctcat
    11341 gagcgatgcc tcacgatgat cgagcaagtt ggaaatacct cctcggcttc attgccgatg
    11401 gctttagacc atgcgaatcg ggggcagaga ctgaagaagg gcgatcaggt catgttgggg
    11461 gcctttggtg gagggctgac gtggggcacg gctttggttc ggtggggata aaattgccct
    11521 ttgggcgtag gtccacttac cggagaaacc tggttgggca ttgtgttgta ttgacgatct
    11581 cttgctattt ggcctatcgt gcgcaagcaa taggcatgag tgggatagag gtagataatt
    11641 aagaatagat aaaataagga ggggtgcgtt gaaaatcttc ctggccaatc ccagagggtt
    11701 ctgtgcggga gtggatcgag cgattgaaat cgtcgatttg tccttaaaga cctacggggc
    11761 accaattttt gttcggcatg aaattgtcca tagccgtcat gtggttgagt cccttcgtgg
    11821 aaagggcgct atttttgtgg aagaactgaa tgaggtgcct gatggcgcca ttgtcatttt
    11881 cagtgcacat ggtgtggcaa aagaggtgtg ggaagaatcc acccgacgaa atttgaaagt
    11941 cattgatgcc acatgtcccc tggtcatcaa ggtccataac gaagtcaatc gggattactc
    12001 caacgaatat gagttgattc tgattgggca cgcgggtcat cctgaggtgg tgggaaccct
    12061 ggggcaggtg cctgataaat ttcatttagt ttcctcggtt gaggatgttg actccttaga
    12121 agtggaaaat cctctgcatt tgtcgtatgt cacccaaacg acattgagtg tggatgagtg
    12181 cagagatatt gttgccgcat tacatcgtcg atttcccggt attaaggggc cacaccagga
    12241 agatatttgt tatgccacgc aaaatcggca aaatgcggtc aaagagctgt cacgttttgt
    12301 tgacgtcatt ctggtcatcg ggtctcctaa tagctccaat tccaaccgat tgagagaatt
    12361 gggcgagcgg tgtggaatcc cttcatatct cattgattct tatcaggata ttcaaactca
    12421 atggctggaa ggtgtgaacg caattggcat tgcggcgggg gcctcggctc cagaagtgtt
    12481 ggtagctcag gttattaagt ttttgaaaga gcaaggagcg acatcggtag aggagttgac
    12541 ggtggtggaa gagaatgttg aatttcttct tcccaaagaa ttactgatgg caaaagtgcc
    12601 gcgataagtt cctctggatt tccgctttcc aggtggaggg cgagcgtatc ttttccgaat
    12661 ttatccttcc tcattatctt tcccaatata ctgaagccgg tggatctgtt tgattgggga
    12721 ctcctcatgg caatagagga acctccgtaa aaggtttcat ggttttatcc tgttgacagg
    12781 aaccttggtt ttggcaatat gtcaagatat tgaataggct gctagtcagc cctaccgtat
    12841 tttgtggaaa attttgattt ttctttcttc tttatggtac tgatttgagc caaattactg
    12901 ccctgtcatc cactccattc tgggagggaa tatgtatctt cgtactctag tcgtcagtgg
    12961 gtttaaatcc tttgctgagg caaaaataga ttttccgaat gggataaccg ctgttgtcgg
    13021 tcccaacggg acaggtaaga gtaatatcgt ggatgccatt ctgtgggcga tgggtgagca
    13081 aagcgtgaag agtttacgaa gtgagcgcat ggaagacgtg atttttaatg ggactgaaca
    13141 acgtaaaccc atgggaatgg tcgaagcctc actcattttt tccgaggtga cgcctcggga
    13201 gttggagcct gtctctgccc ttctggaagg gcttgaccag gcaacggatg tcatgattac
    13261 ccgccggctt taccgcgagg gagacagtga atattacttt aataagattc cctgccgact
    13321 caaggatatt cgaggatttc tctggaatgc ccgggcagga gctcgagggc agtctgtcat
    13381 tgaacagggc aatattgaac agttgctcag tgcttcccca caggaacggc gtgagtttat
    13441 tgagggcacg gcaggtatta ttcgatataa aaagcaaaag gcagaggcgt tacgaaaatt
    13501 acaaagtaca gaaaataatc ttttgcgggt tcgggatatt ctaggagaag ttcgaagcca
    13561 gcttcggaca ctcaatcgtc aggcgaaaca ggcagaggag tatcagacgt ttctccgtga
    13621 agctcgggag cttgaggtgc ggttgttgac ccatgacttt cgccggtttt ttcaagacca
    13681 gtgtcagttt gaaaacgagt tacacgaatc tgaaaatcag gagctttcct gcgtggctga
    13741 agaggccagg cttgtggccg agcaccaaga ggtgcaattg gagttgacgt cttctagcga
    13801 aattctcaag gaagcgcagg accgatttcg agagattgag caacaaatgt ccaatgcata
    13861 tacggcgatt cagattgaac gcaaccggct tgatcagtat gaacagcaac atgagcaggt
    13921 catggaggag cgggctcgac tgaatgggga ggggcaggat gccacgggtt ccttggaagc
    13981 tctgagagcg cggctggccc aaattcggtg tgagatggaa attctctctg tcacggtgaa
    14041 agacggagag ggggccatag cggatctcgc cgttcgccgt cgggaaactg cggagaaggt
    14101 cgacaagggg cgagaaacca ttctggcttt agcggtagat aaaacaaatc aggaaaaccg
    14161 cttgagaagt attggtgaag ggcagagttc gatggccagg cggattgaac gattggctgt
    14221 ggaatatgct caatcgcaaa ccgatcaaac cacacttcaa tcggaaagcg aagcccttcg
    14281 acgggaatgc tcatctctcg aaagtcagct tgatgaactt cgaaacaatc aggatcgctt
    14341 agaggtcaac ctccaaagtc agcgagaaac cagggaagag cttgatggaa aaattctcga
    14401 cttccaaacc caaacagctg gtgctgaatc tgaattacgg gccattcaaa gcgtctttcg
    14461 ggaagaaata gggtatggcc atcatggtga gggtgatgaa gcctcaattc gcgtcgcgtg
    14521 ttcgtgcatt caggaggcgt tggccgaacg gatgggagtg ccagaggatg ttgaaaaagc
    14581 catggaagca gttctgggtg agcggttgca ggcgtggatt gtccaatcgc cccaagaggc
    14641 agaaatgtcc attgcgcaat tcaagcaaca tgaatgggga aaaggcagct ttattccctt
    14701 gaatattccg cgacagggtc gaggggggcc agccgattgg tggagcatcg ttcagcacga
    14761 cacggaggtg ctgggcttgg ctgtggattt tgttcaggtt cctgacgagt tgaagcctgt
    14821 ggttgaggcc ttgttgggga atacggtcat tgtcaaatca ttggctggag cgttgaatct
    14881 catgaccagg cattcctggt tccaagggaa cggtccgctt ttagtggctt tggatgggga
    14941 gcttgtgtct ccatccgggg ttatcagtgg tgggtctggt ggagaggcag gtggattatt
    15001 acgtcgtcgt agagaaattc tcgcattgga agaaaggttg aatactctct ctgtgggatt
    15061 ggaggaagcc aaagccaacc ggaaattgct tctacaggaa attgaggacc tgtcgcaaca
    15121 acttgaagaa gccacgttgt ctattcgcga gatggaattt cagatgttga ccattcaaaa
    15181 agaggcttct tccaaagaga aggctcttcc cgatctcgtt cgtcggttag atgcacttca
    15241 gcaggatcga ttagcggaag aggaggaatg gggccagctc caggttgaag aggtagaggt
    15301 tcgaacccgg cttgagcgtc ttaatcagac acgggctcag gaagatgagg ctcttcgtca
    15361 actgttggac tctctcaatg cattagacca agagcgacaa gacatgttgg aaagcctcaa
    15421 tgatactcga atgaattttc agtctctgaa gtcccaatgg gaccatgaac aagctaatgt
    15481 tgaacggatt gaacgggaag aagagcatcg gaacagccgt attcgacaga ttgatggaca
    15541 attggaacac ctctttctcc aaagtcgaaa aagccaggag gaacgactca ccaacgaaga
    15601 tttggtcgaa gaattaagca ttcaaaaaga ggctattgcc ggaacattat tggagctggg
    15661 gaaccggcat gcagaatgta tggagggagt gaaacgattg gatggtctga ttgccaaggc
    15721 tcgtgaaaca ttgagccata tcttcaaatc ccgagtgcca attgaggggc gtttagcaga
    15781 ggttcgggcg aagttccatg ctgtgcaaga aacgctgacc atgacgtatg aaatttcgac
    15841 ggatgacctc aaatattccc aggatgcgga gcagactcct gaagtgctgg aagggactga
    15901 acaggcatct gacgaggaga aaaccgggca gtggagagag cagttgcagg gtatcagaaa
    15961 aaaattagag cggatcggtc cgattaacct ggccgctatt gaggagcatg ctcaattaga
    16021 agaacgcttt caatttttat tagcgcaaga agaagattta gcgggttcaa tccaatccct
    16081 tcaagaaatt attcagcgat taaatcagac aaccaataag ctatttgtgg atacctttaa
    16141 agagctgcag gtgaaattta gtgaagtctt ttcagccttg ttcgctggag gacgtgctga
    16201 gttgattctc gtggaagaaa ctcaggaagg gcaggaatct gaagcgccag ctcttgagcc
    16261 aggtgtggaa attgtcgccc aaccaccagg gaagcgcttg aaaaacctca atatgctctc
    16321 tgggggggaa aagacgatga ccgtgatggc tcttctattt gcgagctttc tgattcgtcc
    16381 ttctccattt tgtgtattgg atgaggtgga tgcgccatta gatgaaacga atgtggtccg
    16441 gtttggtcaa tttttgagac aaatggcgga ccggtctcaa tttattgtga tcacacacaa
    16501 taaacgcacc atggagactg ccaactctct gtttggtgtg acgatggagg atcctggtgt
    16561 ttcgaagttg atagctgttc ggctccatga attagaagag gtgagctgag catttacagt
    16621 gagtcccatc atttttggga agtctcaaat aaaaagcccc ttccgtagaa tgtcggaagg
    16681 ggcttttttg ccgaaccagg ttttgtattt ggttagtttc gaacgccggc ccaaactttc
    16741 gctgggtcaa tttctttgtc aggattcagc tctttggcct tatccagtag cgtatcagtt
    16801 gtctcaggga tttctggatc aggtatacag caatcatcca caggacagac tgccgcacat
    16861 tgaggctcat caaaatgacc gacgcactct gtgcagcgct cataggtaat cacgtatacc
    16921 gtgtctccat caccctgacc ttcaccgacc ttatggccct tttcttctgc ggcgctacgt
    16981 gtctcaaaaa ttgcttcgtt gggacattcg gggagacagg caccacaatt aatgcactct
    17041 tctgtgatta gcaacgacat aattcctcct taggggacag gtaaaccgtc atgtcaattg
    17101 taggaagcca acgcctacga tttttaatga aaattccctt ggcatgaggg ctcgcacggt
    17161 atcaactctt tggcgatttt aacggtgtaa atttatttgc gtcaaccgga cagaagacct
    17221 atctgataag ccattcgggc ctgcggtaaa attaggtgga ctcaaaaacc attttaagaa
    17281 attgttgggg ccaataacca tacaaattat ggacacatgt ctatattttg aggaaaggca
    17341 gtaatttggt ctgaccttga cgaacctttg gacgattgtt atagcaccga tacgtcattc
    17401 ataacggacg agaaaggttc cgtcgcattt agagagtcgc gtttgaggtt tgagggtgtt
    17461 tgagtaatat gaagcgaagg attatgtctg tgtggatgaa cccttggtag gctcatctca
    17521 gggtattttc gtgatcgggg ttgtttttcc tggtagtcca atcggggtta gacaccttcc
    17581 gtatttttct tcccttttct tgcctgcttt cctgtgaaaa caaatagctc aggatcctca
    17641 gtgttttccg cctatgttgc cctcacgacg gcggcgatgg tctggggagg atcggtggtt
    17701 gcgcaaaaag tcgctttagg tccattctcg cctgtagagg cgtctgtttt cagaggatta
    17761 ggagccttac tgatccttat tcctctttgg ctgtggaagg aaggaattgt ttctttttcc
    17821 cgaagagatt ggcggaattt ttttctgttg ggattgggag tcctgggaaa tcacctgttc
    17881 gtgctatttg gcctgcaatt tattggggca ggggcagcgg gtattattat aggtgcgagt
    17941 ccggcaatta ccgcctttct gtcctcgtta atcctgaagg atgtacctct ccgggtaatt
    18001 tggtttggtt gcctggtttc gttttttgga gtattgagta tttccgggcg cgaggcggtg
    18061 gagggattag gtaccaaccc ctggctcgga gggagtctgg tggtgtgcgc cttagtcagt
    18121 tgggccctct atacgattgg ctgtcgcaaa accatggaac ggttctctcc gctgaccgtg
    18181 acgtggacca ctcttctgat ttctttgatc tttgaaatcc cgttgttggc catgaatcac
    18241 aaagtattgg gtgcggggtt ggcgacggtt cccgtgtctg gttggctggc actcctgtat
    18301 gtcatggtat ttgccacggc gttgggtcaa caggcctggt tgtatggcgt caagggcatt
    18361 ggcccttcca gagcaggaat ttttggcaat cttattccgg tgtccgcctt gtttttttcc
    18421 ctagtgattt tgggagagcc ggttggcatg agggaaataa gtgggatagg attgatcttg
    18481 ctcggggtct ggttggttga tcgacaatcc tgtgccgtat tagtgaaagt tacatgatct
    18541 ggaaataggc ggaaacgaag aaagagagcg cactatgctg aaggaacgga ttgaggaagt
    18601 cacacagatg aatgagttgt tttatcgtgg gtttgaacag ctggatgttg cgctgatgga
    18661 ttctatctgg gctcatcagg agtatgtgac ctgtattcat ccagggtgga gtatacgagt
    18721 ggggtggccg gcggttaggg attcctgggt ggtcattttc aataatacat tttcgatgaa
    18781 atttgaattg actgaagtcc aggtccaagt tgcggctgat gttgcctggg ttatttgtac
    18841 agaaaacatc accagccggg tgggagagaa cgaacagaat agccaggtag tgtcaaccaa
    18901 tttatttgag cggattggag acgaatggaa gattatccat catcacggat caccgctcat
    18961 ggaataagat ctgagtgtgc cttggcggtg ggttacttct gaggcattca attcactatt
    19021 tattagtaaa tttcttacat tttgtggtca atcgggaagg tgcaaggcca ggataatggc
    19081 caaacaggtg tggcttggaa aatggagttt tgatcaggag gaagagctac tgaacgttga
    19141 cttggcccat tcttcaacaa tcacatctcg ttcttccatg ttcagggcgc aatatttttt
    19201 aaacataccc agtccgcctt tttttctgcg ccacgtgacg aaatgtaaaa agtggtcctt
    19261 gcataacgat tgggttccgg caggggcgta gaggggttcg gtgcatcccc caatcagaca
    19321 cgtatcagca ttcatatcct ggtcctcctt ttaagttcgc ctggcagtgt tcaacagtcg
    19381 aagtatgccc aaccgcgagg ggagaaggca atcaattcac gccaggacga ttaagaattt
    19441 tacgtgaatg ccattataga ggagatgggg gtgttccctt gctttctctg tttaatggcg
    19501 ggtatcgtag gcacccagta agcgaaccat ttgacatata ttgacagtgt tgttgaagaa
    19561 aggagcgtgt tctgtgttga ctgctacaga tgtatttgaa caaacaagag agatggcctt
    19621 gcgcgccacg agtcaggaag aacgggctct tcaaatcgat tatccggcgt tgggcaagaa
    19681 gattgagcag gcgttgacag gccgaaaaat taaccttttg tatgtgaatg aatttttgcc
    19741 ggaagggtat gaagatcaag ggcgttttaa cgttatggtc atgaccgctg gcaatgttct
    19801 ctttgatatg gttatcggag attcgtattt tcgatacgac attttttctt gctgtgattt
    19861 agataagatt caattgatcg acgggacatg ggataacaag gaaaaacgca cggaagaatc
    19921 atttttgagt ctacggttaa tgcatgggga tgaggcacat attcttctcg cactcaagga
    19981 cgagcaacgt ccggccgtgt tgtcttttgc cgaaaaaatc tctcatagtc gacatccaga
    20041 aaaagtctga tggatttttc tgaggtctga ataatcgttc ttgaatctcg taaccgctta
    20101 aagggcggta aacggcaagg cacagacaga ggccggaaag cgttggtctc ggatctcgat
    20161 ttccagtttt gtctggggtt gggtgaaatc tcgtagggga aatcccagtg caatcggtct
    20221 tttgatacga ggggagtggg ttgagcttga tacccatccc acttcccgcg ttccactaaa
    20281 aagtttcgcc cccgcttccg gtagttgtgg gggttctact tcaagaacta accccaccat
    20341 tcgccgtttg accgatccgt aggtatccat tctggctaca acttcctgcc cgggatagca
    20401 tcccttcgat aagctaaagg cgataccctc caaattcgct tccggcggaa cgattttttc
    20461 attcaagtct ggcccaaggc gaggcaatcc cccttcaatc ctggcggtct cgagtgcctc
    20521 ccttccgacc ggctgaaggc cggcctcttt ccctattgtt aagagatgag accacagcgc
    20581 gggaagacct tcggcctgga caaataattc aaagtcctgc ccacccgttt cttccgttcg
    20641 agcgattacc ccttgcatgt caccaaatgt gaatgaaatg gtatctaaaa cctggaggga
    20701 attcacttct acaccgaggg cttgtttgag aatatccgga ccattcggcc cggtaaccag
    20761 gaggagtccc caactttcca ggccattgtg gagtttggct tttgtcccat agagcagaaa
    20821 ttttcggagg gcttgaaagg tcaggtcatc cacctctccg acatcctcaa tgatcaacgt
    20881 ctccggttgc agaaatactc gaaaatagga aagcattttt cctttatgat tcatgaaggc
    20941 cgagtaccga ccctgaccag gcttaagagg aagaatatcg ttgctgatga tgctttggag
    21001 ccatgagact cggtcatcac ccgtgaccat gatttttcct cgtaatgata gatcggcgag
    21061 gccgcctctc tgatgaatgg actcatactc taacagaggg ttgccgtaat gaagggggac
    21121 ttcccattcg ccacaaggtt gaaacctggc gcctagggat tcatgctgct tatgaagggc
    21181 aagggtcttc atgatgtcgc tgccgtcgtg atttcctcaa atagcgcgag tgtccgcgca
    21241 gaaaactcat tgcgcgagac aattttttga attccaaggt ctttcgccag tttccatgtt
    21301 tgaacttctt catgattggc aaaggccagg atgggtaatg cctgtgtttg aggaagtgtg
    21361 cgtagcgatt tcacaatatc ggatgcattg agccgatcat cattcatgtt gacgataatg
    21421 cccatcggtt ggctggcaag agctttcgtt tgccagtcat ctccagtgcg gatacgttcc
    21481 agtttatagc cttgcggtaa aaaggcgtcg cgtattttgg tgtagaagaa gatatcacta
    21541 accgccacaa gaatcgtttt ggtgtctgac atttggtaag gcctttattc gttctagttc
    21601 ataagagtca gaatgcgcat gagcgcttgt tttgctgtct tgtattcagg gttaagagcc
    21661 agggattttt tgtaggaatc gactgcctgg tcccattccc cttgtcgttc atacacacgg
    21721 ccgagattca tgtgaggaaa agaagggttt tcatatcgtt tggctttcat cgctttttca
    21781 agccagggaa tagcctcgtc cagctggttg agctcaataa ggtaagcccc gatatcgtta
    21841 tagggatttc cgaattcagg gtcctgacga atagccactt ggcattcctc aatggcttcc
    21901 tgcaattgtc ccatgaaact ataggcccat cctaaaaacg tatgtgcttc tgcagtcggg
    21961 aaaatgtcga tggattgttt atagagatcc acggcctctt ccagttgccc tttcatttgg
    22021 agttcatagg cctgctgaaa aaggttccag gccgtcacct tatcttcttc gtggccctcg
    22081 ccctggtcga gaaaatcttg aatatccata ggaaattgtg ttgtgaacag tcctttttgt
    22141 gtctcttagg aaaaaaggtg attattcgcc tttgagttta ctctgaacca actcagctgc
    22201 acgtcggccg gaaagtaaca tcgacccaaa cgccggtccc atccgtggac tgccatcaac
    22261 tgcagcaacg gccaagccag cgacaaagca atttggatag atttcacggg tattttctac
    22321 cactaatact tcggaacgcg ccacccacat agccccgttt ccaggcacct tctgatataa
    22381 gttacgttgg tggaggagat taaccacgac ggcatcatga ccggtggcat caaccacaat
    22441 ttgactttca agggcaatgg gatctacgtg aatggtacca tgccccgcca tttccacggt
    22501 ggaattattg accaccacac cttcgattcg ctgatcttgc cgaagaatga gatcgacgac
    22561 tttggtaaga ttcataatct tgacgccggc ttcgtaggca gcggagatga gccggctggt
    22621 cacatgaggc ggatcaacca tggtcattcc cgggcagtct ttaatcggtt taaagggaat
    22681 gcctaattct tcaagaatcc gatccgctgg ttcgcaaatc gtggccttat tcatgagaaa
    22741 accaccagac caaaagccgc caccaagagc tagtgattgt tcgatgagca gtgtccggaa
    22801 accggtcgcg gcaaggtctc tggcgcagag gagtccggac ggacctcctc cgacgatgat
    22861 gatatcgctt tcaatcagtg agtcgaattc tttgtagtac tccctggcaa tgtgacgggt
    22921 gatatcccgt tctcgtaaag gagcgatgtt gataggattc ataggggagc tccagataat
    22981 atttcgtttt tatggaacat caagtacaat cacgttttgt ttttgcctat taccaagact
    23041 ctgttgatca cctgcttgtt ccaccatgga cttttaccgc tttacagata taagtctcca
    23101 ccggttttcc ggaactcttc ggatttttcc tgcatgcctt gtttcagtgc ggcctcatct
    23161 gccaattgtt tttccgcagc atactctcgc acgtcctggg taattttcat ggaacaaaaa
    23221 tgcgggccac acatggagca aaaatgggct agcttcgctc cttgcgcggg gagtgtggca
    23281 tcatggaaat ccttcgccgt ttccgggtca agggataaat tgaattggtc ctgccatcga
    23341 aattcaaagc gcgcctgaga tagggcattg tccctgtgtt gggctccggg atggcctttg
    23401 gccaaatctg ccgcatgtgc ggcgattttg taagccatga ctccggcctt cacgtcttct
    23461 ttgtccggta ggcccaaatg ttccttgggc gtgacgtaac acagcatagc gcatccatac
    23521 cagccgatca tcgccgcgcc gatcgccgag gtgatgtggt cgtagcctgg agcgatatcc
    23581 gtggtgaggg ggcccagggt ataaaagggg gcttcgtcac agacggaaag ctgcttgtcc
    23641 atgttttctt taatgagttg catgggaacg tgtcccggac cttcaatcat gacctgcaca
    23701 tcatgcttcc atgcaatatg ggtgagttct cccaaggttt ccagttcggc gaattgagcc
    23761 gcgtcgttgg cgtctgccag agaaccaggg cgcagtccgt cgcctaaact gaaggataca
    23821 tcatagcttt tcataatctc gcagatttct tcaaaatgcg tataggcgaa gttttcctga
    23881 tggtgagcga ggcaccattt ggcatggata gatccgcctc gtgagacgat gccggtcatg
    23941 cgagaggcgg tcagtgggac ataggccagg cgaacaccgg cgtgaatggt gaagtagtcc
    24001 acgccttgtt cggcttgttc cacgagagta tctcgaaaga tttcccaggt aagttcctca
    24061 ggttttcctc caactttttc taatgcttgg tagatgggaa cggtccctat aggtactgga
    24121 gaatttcgaa taatccactc acgagtttcg tggatatttt tccctgtgga gaggtccatg
    24181 acggtgtcgc caccccatcg gatggaccaa atcaattttt caacttcctc ttcaatagaa
    24241 gaggacacgg cagaattgcc gatgttggcg tttattttaa ctaaaaaatt gcgcccgatg
    24301 atcataggct caatttccgg atgattaata ttggcaggaa taatggcccg tccccgggca
    24361 atttcatcac gaacaaactc cggggtgata taggaaggag tggacgcgcc ccaattctgg
    24421 ccaggatgtt gagtcacccc aaaggtttgt ccggtcccgt gttgggcgga ttggtgttcc
    24481 tctcgtgcct ggttctcccg tatggcaata aattccattt ccggtgtgac gattcccttc
    24541 cgagcataat gcaattgggt gacatttcga cctgattttg cccgaagggg tgggcgttgc
    24601 aaatggaatc ggatggcctg taaggcggga tcgttggccc gggttcgtcc atactgtgaa
    24661 ctgacctctt ttaaaggttc tacgtcttgc cgttccgcaa tccaatcctt tctgagggga
    24721 tggagacctt tgcgcacatc aatgtggaga gcaggatccg tatagggtcc tgaggtatca
    24781 tagacgatga cagaagggtt ctgaacttca gtgtggtctt gaggactatg acttggggtt
    24841 tgatgaatct cgcgcatagg gactcgtaca tctggcctcg agccggttcg atatattttt
    24901 tgtgagcccg gaaatggttg aatcgataag gaggaaagag gaatacgggg ctcctcagaa
    24961 acgacgcgca atgaaggtgt tggtgggcta gtggttgtgg cttggggtgg tgtcatgcaa
    25021 accctctctt tctggggtta ggaattgatc cagggagctg actccaaaat acaaaagccg
    25081 cccctttttt tgctaaagag tgcggctgct cgtgcgggct ttcgtgtctc ctgcttccct
    25141 acgctggtat tatccaggtc aggtcgtgag ggtcgtcccc gcttttggga ctctcagctt
    25201 gtcgcacccc tagcaaactc atggtaagtc tcgttctgtt tgtttgtcaa acagtgccag
    25261 tcccctattc cctcaaagag aagtaatgtc aacctccatc tctaagtttc ttgtacactg
    25321 cggttatatc ctcgattccc aatcgaatgt ccaaattggc ctgacaaaac tctatgccac
    25381 gaccaccaag ttctcttcca tggtcaggca gtgtcaggat ctgtgctact gccgcagata
    25441 aaccagcaac acttttattg ggtactaaaa tcccgtgacc aaccgttttt aaaagctcgg
    25501 tttcacagcc cgtatcaaac cctatgatgg gtaagcccat agccatggct ttataggttg
    25561 aaagattggt ctcctcaaaa atcatggtac gaagaaacat tgtggcggct gcgtaataag
    25621 gtaggaggtc atcctgatag ccaacgagat gcatatgggt ataagtgcct aaatcttttg
    25681 cacggttttc tagttctgca cgttgcttcc catcgccaac caaaagccaa tagagcttag
    25741 gaaactgttg tacaaggctg gaaagagctt ccaaggcata tatgtgccct ttagaggagt
    25801 ccaggcgacc gacagaaagt gcaataaaag catctgaagg caaatgataa ttttctctga
    25861 ttgtcttatg gaattgttcc cgtttatgtc gaatcttgtt gatttcttca aggtcaagaa
    25921 tcccgcgaag aggaacaatt ttggaattgg gaatgcctac tgcatgcagt tcatcctgac
    25981 agcgggaggc agcagtgaag aaacaatcga ataagccatg aaaacgggca tacagtcttg
    26041 gaacccaggg ttttccttca tccaccattt gggaaaagag tgacggaacc agatgaacca
    26101 ctgggcgccg caacaggact tttaatggca cgccaatggt taaggcataa gttgtatgta
    26161 cattaataat gtccgggcga aaccagatta aggctttaca gacatgccat attgttgaaa
    26221 tgtaccggcc aagaaaccaa gtcagggacc aacgatctaa aagttttaag atacgccgat
    26281 taggaacttg gccataggca cccttctctc gcagaagatc tgccgttatc cattttcctt
    26341 tatcaggcga aaagctaatg actaagtgtt tgaccttatc cggtggggtc aagcgcacaa
    26401 tatcccagag caatcttgtt gtgccatagc cggttattct atcaatcaga tggagcactc
    26461 ggaccggtgt atcatccttg atctttttgt tcacttttcc atccccgtct gcttccgaag
    26521 aggtctcgtt gaaatagggc actaaattac tgccttcgtt ttttaatgca tgcttattcg
    26581 cctgatatgg aaatttgtta gataggtcca tgaaattagg gtcagaccaa attggtacat
    26641 taaaattggt acattaagac cccaagcaaa gtacgagatt ccaagagact ctgtaattgg
    26701 gtaaaaccga cggcttagac tctcgctgta tttcaatcat gttaaatttc ttttttggaa
    26761 aatttaacca tctgcaaagt aatcatttca gcaattattt tatgggcgag ttcatttggg
    26821 tgtgtatcaa cgagagtcgg gggttcacca cgccaatggt tgtagtgtcg ttggaaactt
    26881 ttttcatcga tggttggcag ccggtttagt aactcactgt gcgcatctat tactaccgca
    26941 ttcctcggga aaagatcttt tgaaattggg acaggaagat gattttctcc cagtactact
    27001 atgatcatcc tggcgttatt ttgttctgcc atgttggcga tttctttata tgcgtcttgt
    27061 atgaccgccg agcgattttt tgtgggacca tttgtcaatc tcaaaaattt tgcgagagtg
    27121 tattttgtca tattgaaatc atcgtgaagt aggagaggaa agccgatatt caccgaaaaa
    27181 agaaaaaaat cagaaactcc tttttgggta tttcgatagt tgtcgatagg taagtcaaat
    27241 accttggttt gaaagacggc gggtgctatc cctagggcgc tttctccaaa atagtagggg
    27301 ttagggagct ttccgtaata cgaaggcgca aagggttgga ttgctcgttt cacaagccag
    27361 ggcgaatatt gaacaatgac atagtcaggc ttatggcgag gaatcaatag tttcgcaatt
    27421 aacaccattt gtgaaaggcc gtaactacaa acaccagcat tctttgtcga tacgccgaaa
    27481 tcggcgccaa tcaggtaggg aaaggtattt tcagcatgag tggcagctcc atacgtgaag
    27541 gagcatccta aggtgagcgc atatggatgg ttgtttgtgg tctttaactt ttcatccact
    27601 ggaattctaa atccgtcctt gtcatatcgt accggaatgt cagggcctaa aggcattatt
    27661 tcccagccat gggaattggg aataggtgca tatccaagtt ctttatgagc ttgatggact
    27721 tgtccgctcc aaccacgctg atcggattta acgtagttat agaattggac tgttctaaac
    27781 gttgtgtacg caagataagt taatacgagg gtagtgatcg ggataagcaa tagaactata
    27841 tgaaaaaata tacttttctt cgtggaaggt ttctgcttgg acatcaccaa taccccagct
    27901 tcaagaggca atctccatgg atctcactaa ctttcggtaa ctggtaaacg gacaccgctt
    27961 tttggcaatt gatcaatggt agtttttcca aatgtggatt ccgctcccca atgtttgagt
    28021 tcctttaaac tgtaattcct tcggttcccc atgccccccg ctcgccaagt attgggtttt
    28081 ttagatttat ccctcaaggc agaggtagac cacacctaca attgaaccca gaactacaat
    28141 tgaacccaga aatggtaata atgctgaagc gattccagtt tgtacccaaa cctttcataa
    28201 aatttacagg ctcgtatatt gtttttctgg gtaaccactt ctgaagtatg tataccattt
    28261 gatatcagcc attcatgtag agtcttcatc aacaatgagc cgattccttg accttgaact
    28321 tttttttgaa cccccatcaa gccaactttt ccaatgccat ttttgacaga cccggtcacc
    28381 attccgagac aatcttccat atttgcggaa ttcttcacga taaacaccac atcggcaatt
    28441 tcatgtaaag tgcttcggtt aatccaaata tggtacatgc ctgcaaattt ttccttcgaa
    28501 atacatggat cgtcgtgaaa tctagaatgg ccacctgcga tcaacgcaag agtttttaac
    28561 tgctgattgg cctgcgatcg agggaattca attacttcaa acgaatgact tgggttctta
    28621 atctcgccga aggtgaattc ggtactcagc ttccgttgat aggtaacttt tctgtctacc
    28681 agggaacctg aaaaactttg aagaagaggc aaagggatca ttctgtccgg gcaagtggcc
    28741 caataaatca agtggtagcc ctgttctttt gcaaaagaca gaatgttctc aagttcagag
    28801 ttcgttaatg ctggagaaat aatctgccca acggaaatct caaaatgttg agagtcccag
    28861 ggtagtggca caagaaccgt taaaagatta ttcttaattt atctcttctc tctttgtgca
    28921 agtgttctac taggtagaac ctcctcgcca ctattggaca aatctgagcg ctggaggttt
    28981 ctctcggttt agctttattt tccctttcac ggtttccaga ccttcggtta tacattttat
    29041 gatgtattcc tgctcttttt cagtcatttc gtaatagaat ggcaaacgaa gcagtcgacc
    29101 gctcaattgt tctgtgattg ggaaatcgcc ctcgatatat ccaaactttt gccccattgg
    29161 cgaactgtgc agtggaacaa aatgaaaaac cgccaaaatg ttctgttgcc ttaagtaccc
    29221 catgagggca tctcttgttt ctatatctgg aagaagaatg taaaacatat gaaaattact
    29281 cttgcaatct cctggtatgg tagggagact caatagacct tgaatttcaa ggggtcgcag
    29341 gagcatccgg taccgtttaa aaattcccct tctgcgctct gcaatcgagt ccaacatctc
    29401 caattgggca tagaggaatg cacagacgat ttcactgggc acatatgacg atccaatgtc
    29461 gacccatgtg tacttatcaa cttccccgcg aaaaaattcg ctccggttgg ttcccttgtc
    29521 tcgaatgatt tctgcccgcc ttatcattcc gggatcgttg atgcaaagtg cgcccccctc
    29581 accacagata taatttttag tctcatggaa actataggtt ccgagatgcc caatagttcc
    29641 gagtgcccgg tttttataat acgcccccac tccttgcgcc gcatcttcta tgaccaagag
    29701 tgcatgtttc tttgcaatcg ccataatgtg gtccatttca catgcgattc cggcataatg
    29761 aaccggcaaa atggcttttg tttttggagt aatcgcttct tctactcgat tttcatccag
    29821 attcagtgtg tcaggcctga tatcgacaaa taccggtttg gcgcccaacc gaacaatcgc
    29881 attggcggtc gagacgaaag tataggaggg gagaatgacc tcatcaccgg gtccaaggtt
    29941 acaaagcatt gcggccattt ccagggctgc cgtgcaggaa ggtgtcataa gcactttgtg
    30001 aacgccgaac tgtttttcaa acagttcaga acatttttta gtgaaatacc catcaccagc
    30061 caaatttcca aacgtcaccg cctgcgcaat ataataaagc tcctttcccg caatgaacgg
    30121 tttattgaag gggatgatct tttccatctt tctctctcca tttgttggaa aatacctttc
    30181 tatgcgtcaa cggcagaggt ctcttgcctg aaattagaat ttcgtgcctc acaccgttct
    30241 gaaaatactt ggcgtaatca cccttggagg gtgttactcc caatgagtcg ttgccccaaa
    30301 cctttttgcc ggttcacctc ttcgaaacac ctacggtaca taccctgctt gagttgcatg
    30361 atttcgacaa agtttggtta cctttacgga gaatttttgg cacctctatc aagccaggca
    30421 agcccacaac caagcatttc tatgtagggt tgttgccgat ctaagtcgtt ggctcacagc
    30481 agggatatct catctcctta cggctgaagg ctttagctat cgggaattct ggctaccgga
    30541 ttacccaaag ggtataaatc tgggaatctg ggattgtgta atgagagcgg ggcggtgacg
    30601 gctttcttcc gggaaaaatt ttcatcccag ggcattagat tttaacatcc attctggaat
    30661 ctaacggtgc catggtcccc cctcgaacaa ctcggtccaa ctttttctga aaccctacaa
    30721 attacccgtt gtcctcaaaa aaacagaata agtacttaat cgttgtcaaa aaaatcggcc
    30781 accactgagg aaggagcatg cttttttctt gaagtttacc tgaaaggcac actaatggga
    30841 attcccaatt tttcttcatc ccctggcaat accaatacct caaggatttc attgggagat
    30901 tcgatatacc cggtatattg ttgatttcgg cctgtatcag cctcgacaga ggcaattagc
    30961 ttattgtccg atccaaggtt gcagggtttt tccctcaacc atggacaaat tctttcgggt
    31021 ttccaaatcc agggataacg ccaagcctaa atatgaggca atgccatgaa gatacagagc
    31081 gctggaagtg tgagtggtag gcgcacggga cattttttag tcgtggagta aatggtgttt
    31141 cagaacttat taacgggacc ttgttaataa aaatgacgga aatcttaggc tgtagaagta
    31201 aggctggtgg aaacataccc gatataagaa aaggcctatt ggttgaactt ggattggaca
    31261 tcatccttat gccgagattg tacaggtggg ggaatgtaat gaacgttttg tcagtcggct
    31321 attccggcaa tttatgctaa tagaagataa cagattctcc tggactgctc cttcatcaac
    31381 aggcccatgc ggattttgaa cttggttggt ctgtattgat tccagtgcag gctctttttg
    31441 tgcaccattg ctcggatagg gaagagtggg attgatatgt ctcggtggca aaaccgattc
    31501 cccagggaaa aggcagagag gtatctgtgt gtatagcacc aaagggaaaa cgcatttagc
    31561 aaaagattaa cagaccgtct ttttgattta tcgggcttaa taaaacactg gaggtcgagc
    31621 cttctgtagt ccccgcaggc acgggagtcg attgtcggca ggtggattgt tgtggagcag
    31681 ctcatatagt agctatttaa ggagaattcc tttcataggt gcctacagat ttggtaagct
    31741 taatttctgt tccttgattt cgttaaattg gtagttagta tcagaaaaaa cagccatctt
    31801 tgaacaaaaa actcgaaaac gtccttggaa ataattatgc ccactattac agaattccct
    31861 attactgact accaaactct tccggatcag gaattgtttg atcgaattgt cgacgcaaaa
    31921 gccatattag gtcggaaagt attgctattg ggacacaact atcaacggga tgatgtaatc
    31981 atccatgccg attttcgagg cgattctctt ctcttggcca agtatgcggc tgaacaccct
    32041 gaatttcctc atgtggtgtt ttgtggggtg catttcatgg ctgaaacagc cgacattctc
    32101 agtcgttcca atcaaacggt cattctgcct gatatggctg ctggctgctc aatggcggat
    32161 atggcttcca tggaacaggt ggatgattgt tgggagcggc ttgcacgggt gttgcctgtt
    32221 gaggagaccg tgacccccat cgtgtatgtg aattcagctg cttctctcaa agccttttgc
    32281 ggagaacatg gtggggtgac ctgcacttcc tcaaacgcca aagccattat gaactgggcc
    32341 tgggagagac gggagaaaat tctttttttc ccggatgagc atttagggcg gaacaccgcg
    32401 aatgcgatgg gatttccccc tgaggagatg atcgtctggg atccttttca acccaatggc
    32461 ggacatactc gcgaaaccat acacaaggct cgtctgattc tctggaaggg gcattgtagt
    32521 gtgcatcaga tgttccagcc ggcacacgtt agctattttc gaaagcagta tcctaatgtt
    32581 caggttattg tgcatcctga atgtcatgag gatgtggtca atcaggcaga cctggtggga
    32641 tcgacggaat ttattattcg gacggtgact caagccgatc caggaacaat ttgggcggtg
    32701 ggaaccgagc tgaatctggt gaatcggttg aaacatgatc aagccgataa gcaagtcttt
    32761 ttcctttccc caatggtgtg tcgctgttcg acgatgtata gaattgatgg gcctcattta
    32821 tgttgggcca tggaaaatct cgtgcagggt catgtggtta atcacatcca agttcctgac
    32881 gaggagaaag tgtttgccaa gttggctttg gaccgcatga tggatttgag ctgaatacct
    32941 ttcttctgtg gttccccccc ttccttcatt tgttgcgctt tcatatcaat aacacggttg
    33001 ttcccattga tgtgttgttg tcgcgatatg actgaagggt gttgtttgac gggtttggga
    33061 ggcatggcta gaatggcctt gagtttatcc cactattctt gagaatttcc ccatggacta
    33121 caaagcgaca ttgaaccttc cccggactga gtttccgatg aaggccaatc tgccccaaaa
    33181 agaaccggag cgattagcct ggtgggagca ggagcgggtg tacgaccgta ttcaggaggc
    33241 cagaaatggc agcccccgtt atatccttca tgatggccct ccctatgcca atgggcatat
    33301 tcatatcggg catgggctga ataaaattct caaagacatc attattaaat cgaaaaccat
    33361 ggcggggttc caggctccct atatccctgg atgggattgt cacgggttgc cgattgaaca
    33421 tcaggtgacc aagcagcttg gtcccaagaa aaaagatctg agtgcggtgg aactccggaa
    33481 gctctgtcgc gagtatgcgg agaaatttgt tgagatccaa cgagacgagt ttcgccggtt
    33541 aggggtattc ggggattggg agcatccgta tctgacgatg gatcatgcct atgaggccgc
    33601 gattgttcga gagtttggaa agtttgtgga aaagggtcgc gtctataagg gattgaagcc
    33661 ggtgttgtgg tgtaccgtgg atcaaacggc gctggctgaa gccgaagtgg aatatgagaa
    33721 tcatctctca ccgtcggtat atgtgaaatt tccctttacg gtcctgccgg ctgaaatggg
    33781 ttccgaccaa cgattgggtc tgccaaccgt caaaaagttg aaagccgttt cggccgtcat
    33841 ttggacaacg accccttgga cccttcccgc gaaccaagcc atttgcctcc atccggattt
    33901 tgactatgcc gttcttcaag ctggcgaaga ggcttttatc gtggctcttg gattggtaga
    33961 cgcatttgcc aaggcctgta agattgagaa atttgaggta ttaggaaaaa agaaaggaaa
    34021 agaattcgaa gggtggacgt gtcatcctcc atttgccaag aaagagatcc ctatcctcaa
    34081 tggagatttt gtcaccttgg agcaagggac cgggtgtgtc catattgctc ctggacatgg
    34141 gatggacgac tatcttttag ttctgaaata taacaaaccc ccttatgtcg atatgcttcc
    34201 tgatcggaag ccattggaaa tcctggtgcc ggtcaacaat gcaggacggt ttacggatga
    34261 ttttcctgag tttgcaggac aacccgtttt ggaggcgaat cccgggattg tggaagtctt
    34321 gaatgccagg aagttgttag ttggtgaaaa aaggcttgaa catacttatc ctcattgttg
    34381 gcgctgtaag caaccggtca tctttcgggc tacacaccaa tggtttgtgt ccatggagga
    34441 ggccgatctt cgccaaaaag ccctgaaaga aattgataaa gtccaatgga ttccagagcg
    34501 gggacgagat cgtatttatg ggatgatttt gaaccgtccg gattggtgct tatctcgtca
    34561 gcgaatgtgg gggacgccca ttcctgtgtt ttcatgccaa agctgttcca cgccattaac
    34621 tgattccaaa gtcatcgagc atatcagtgt tcaaatgcag gaaagtgggg ccgacatttg
    34681 gtttagtcga tccgcccagg aattacttcc ccagggaacg acatgtgtgc aatgcggcca
    34741 tgggttgttt aagaaagaac atgatatttt agatgtctgg tttgaatcgg gggtgagtca
    34801 tgcagcggtg ttgaggcctc aaggtgaagg ctggtatccc gcagacttat accttgaagg
    34861 ttccgatcaa cataggggat ggttccacag ttcgcttttg acatcggtga cgacggatga
    34921 gcaagcaccc tatcgggccg tcttgac
//
LOCUS       DAORAQ010000017        34915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_261_length_34915_cov_71.139587, whole genome shotgun sequence.
ACCESSION   DAORAQ010000017 DAORAQ010000000
VERSION     DAORAQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 34915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34915
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_261_length_34915_cov_71.139587"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1814)
                     /locus_tag="PKM72_03110"
     CDS             complement(264..1814)
                     /locus_tag="PKM72_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HNP59801.1"
                     /translation="MNTTQSPAHTSLVLPADQYNQELVNNVHPPQWRNPTPSGRYNLV
                     IIGAGTAGLVTAAIASALGAKVALIERHLMGGDCLNVGCVPSKGVIRAAKAWTAVREA
                     EQFGVHISGEVKRDFGAAMARMRKLRARISHVDSAHRFTKLGVDVFIGNGRFTSSSTI
                     DVDGTTLQFTKAVICTGARATAPPIPGLANTAYLTNETIFSLTELPPRLGVIGAGPIG
                     CELAQSFARFGSQVFLVEAMHGILPNEDRDAAEIVEQSIVRDGVQLLCCGKDLNIQSA
                     KGAKHLVVDSHGTHYDIPVDEILVGVGRSPNIEGLELEAVGVEFDKTGVKVNPRLQTS
                     NPKIFAAGDICSPFKFTHTADAQAQIVIQNALFPHPFGLGYGSTDQLVIPWATYTQPE
                     IAHVGLYEKDAKDKNIPIDTFTFPLNEVDRAILDGEEEGFARVHVKKGTDKIVGATIV
                     ASHAGDMISEYTLAMKGGLGLNTIASTIHPYPTQAEVVKKSANAWRKTTLTEGKKQIL
                     RKLFAWTR"
     gene            complement(1849..2586)
                     /locus_tag="PKM72_03115"
     CDS             complement(1849..2586)
                     /locus_tag="PKM72_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HNP59802.1"
                     /translation="MSHPSTTPTDAPNSLKGKILIGCILLAGIGAFLYFDLGQYLSLE
                     ALKANRDTLLAYTASHYETAVAVFILVYILQTAFSLPGGAILTLTGGFLFGSLMGTLF
                     VNIGATAGATLAFLAARYLLHDWVERKFGDRLGTIQEGFANNAFSYLMTLRLIPAFPF
                     FLVNLVSGLTRVNLGTYVLATSIGIIPGSFVFAFAGRQLGAINSLSEIASPPVLLAFT
                     LLGLLALMPVAYQKWKKPQSGAINTKS"
     gene            complement(2877..4262)
                     /locus_tag="PKM72_03120"
     CDS             complement(2877..4262)
                     /locus_tag="PKM72_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNP59803.1"
                     /translation="MIHSHDIIIIGGGSAGYAAARTAQAEGADVGIVDQGPLGGLCIL
                     RGCMPTKAILRSSDLAALIRRAPEFGLTSSRLQVNLPEIIDRKDKLIKEFAEDRIQAL
                     HHPRFTLYQEYAHFVSPTIIQVGQQQLSAKAYIIATGSVVSHPDIPGLEEAGYLTSDE
                     ALELHSQPESMIVLGGGPVALELAQFFSRIGVAVTLIQRSTHILSDTDEDLVQPVEAH
                     LRAEGMTVYTNTCLQQVQASGEMKTVHFLHQGQPRHVTATSILHALGRRPNIDGLNLD
                     AAQIAHQPNAILVNKEMRTSQPHIFAVGDVNGGYEIVHIAIEEGEIAGWNAVHANLPP
                     KHFSDRLKTQVVFTDPQVASVGLSERECLDRQLPYLVASYPFNDHGKSLCLGETYGHV
                     KVLCHPQSGEILGGHIVGPEASELIHELIAIMHFHGTVHDLLRIPHYHPTLAEILTYP
                     AEELVGKLPSA"
     gene            complement(4764..6713)
                     /locus_tag="PKM72_03125"
     CDS             complement(4764..6713)
                     /locus_tag="PKM72_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59804.1"
                     /translation="MPIRIEKSQLTTLRIFIVDDSPGDRSTYTRWIRRTAPFRLDIQE
                     METGVQAWEACKISLPHCLLMDDQLPDMTGLEFLAKVRGSQSQSKNPIIFLTGYGDEK
                     IAAHAIKLGAQEYFNKNTLSEKFLWHHMEQAIEQCVTIPRIRSLERQSSIILQSSSDG
                     ILVVGHDGLIRFSNPAAEQLFQKTSEQLNGSPFGFPVSANQTMEIEIMSELGRPTPVE
                     MRVVPIEWDGEPAFLASLRDVTEQRRAEDERRRHEIERQYSQKLESLGVLAGGIAHDF
                     NNLLMTIVARSGLALRSLPPDAPAREHLDFIEKAGLRGGELANQMLAFAGQTRLDFQV
                     INLPNLLEDMAPFLRATLSKRLTFEYDLAPSLPPIRADQAQLRQIIMNIVVNASESFG
                     EGDGVITIRTFEWEPATENFQSWYVIGELPHSRGVALTIQDTGCGMGSDTIPKIFDPF
                     FSTKLPGRGLGLAALLGLAQAHAASIAVRSQPDIGTEFTLLFPATTPNPSTKKGPVFS
                     IPTQHASPTKKPMVLVVDDEEDVRVACSMIFEEMGLQTLVASDGQIGIQVFQQHQQEI
                     MVVLLDLTMPNMDGQQFLEHIQGLNATIPVILSSGYSEEEAMKRFHNPNMAAFIQKPY
                     QVETLIAKVEEIRQKQTTSSNNPGR"
     gene            complement(7730..9409)
                     /locus_tag="PKM72_03130"
     CDS             complement(7730..9409)
                     /locus_tag="PKM72_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNP59805.1"
                     /translation="MISLLLVEDDLKEALVIRNMLKEGLQNQFTLENSLSLSDALDLI
                     QQNKFQAIILDSHLPDGKSFESILQFMQFCPDIPILILSGVEEEAQAIQAVKSGAQDY
                     LIKGQTSSSTLCRAIRYAMERQKATQRITQLAHYDHLTGLANRGLFYERLNCAVARCH
                     RNDTAIALMFLDLDHFKDINDTLGHEYGDSLLKTVAARIKKCIREIDTGVRLGGDEFA
                     VLLEQIVSIEDVAAVAHRILHLLAQPVIIKQHQLRVTGSLGITIYPWDSANPQELLSH
                     ADAAMYRAKAQGGNTYQFYTAGMKTAGLDGSTLEMELSRALAKEEFLLHYQPQMNICT
                     KQVIGMEALLRWHHPYQGLIGPNHFIPQAEENGMIIPIGEWVLRSASKQAKYWEKQGF
                     PAPHVAVNLSARQIHQGHLPELIQDILKHSHLDPENLKLELTETFLIHETEETIQTLR
                     EIRAMGVHLYIDDFGAGYASLRYLKSFPLDGIKLDQSLIQNLPYSTNDAAIVLAVISL
                     AKALGLQVIAEGVERQEQADFLEEHGCDAMQGFWIAPPLPANESAQHMVHM"
     gene            10014..11837
                     /gene="rpoD"
                     /locus_tag="PKM72_03135"
     CDS             10014..11837
                     /gene="rpoD"
                     /locus_tag="PKM72_03135"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNP59806.1"
                     /translation="MGKTASLPEVIQRLIEIGKPKGHVTIKDLTSILPADQITSDQLH
                     TILSELHEVNLQVIDPSKRTALQLAALKKNAGQEESDDSEEETDSSLDIDLTPGEPTR
                     IDDPVRLYLKEMGRVPLLTREGEIELAKHIEEGKRELACAVYGMPLNINYLESLHDQL
                     KLEEIRVRDIVLLQETLEEEEVEEEAIASEQEDEELRLRTLESLATVRKLGRGLLSLY
                     IQNRDGQTTKNSKAQLEKRRIGLADQFVDQIEALNLHQRVKDQMLRRVKDVGQEIRSV
                     EIMTARHTKRLGLAGQDGMQVIGKKPRATTKGSSHRRKQQLTPEEIEKLKQEVEAGKG
                     TLLRIQDEVLGMPMEEFTAALTILETAEEKVKRGKAQLIEANLRLVVSIARKYTNRGL
                     QFIDLIQEGNIGLMRAVDKFEYQRGYKFSTYATWWIRQGVTRAIADQARTIRIPVHMI
                     EANTKLARTARQLVQQLGREPTPEEIAERMGLTPEKVRMMLDISKGTISLETPIGEKE
                     DSQLGDLIEDKNAVSPMDAANRYDLQRQIANALGVLTPREETVIRKRFGIGDSTDHTL
                     EEIGQDFDVTRERIRQIEAKALKKLRHPSCSHKLRSLVDHL"
     gene            12020..12694
                     /locus_tag="PKM72_03140"
     CDS             12020..12694
                     /locus_tag="PKM72_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP59807.1"
                     /translation="MRILVVEDEPKVASFIRRALEEESYAVDVCADGPGGLDWAQAVN
                     YDLIVLDLMLPGLPGLEVLKRARAAGVKAPVLILTARSEVDQRVKGLDAGADDYLTKP
                     FAIEELLARARALLRRAGGAPTGILQVDDVILNPVTREVTRAGQRLEFTTKEYALLEY
                     LMRNAGRVLTRSMITEHVWDLDFDTFTNVIDVYISYLRNKIDKGRERSLIQTVRGSGY
                     MMRSDG"
     gene            12691..14109
                     /locus_tag="PKM72_03145"
     CDS             12691..14109
                     /locus_tag="PKM72_03145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HNP59808.1"
                     /translation="MKTTSIRVRLTLWYGSALALILVLFAVALYLVMSRALREQVDAS
                     LDEAAAVAIRTLGEHRFGPFLIFEDLSQDFPEIALLDKFFQIFGPAGQVTIQSANIQS
                     REIPLSQTSFRASLDGKSTFESVQFEKGVPLRLLSVPIRQDDQLVNILRVGTSLYPTE
                     RMLHRLLAGLYIASPLALLVSLVGGWFLAGRALRPVHAITQAAQRIAAGDWTQRIQTP
                     HSNDEIGQLASTFNDMIGRLEVSFRQIRQFSADASHELRTPLTITKGETELALRRPRQ
                     AEDYQAVLESNLEEIDRMSRIVDELLFLSRADLGEIKLKMYPVQLDDLVREIQQQALV
                     LGQERNIHTVLKAIEPVVVQGDDLRLRELLLNLVDNAVKYSHQGQTVELSLLVAGNKG
                     KLVVRDHGIGLASEDHGRIFDRFYRTDEARSHSAKGTGLGLAICKWIVEVHHGTIEVQ
                     SVVHGGSCFTVFLPLAPSAAIV"
     gene            14297..15541
                     /locus_tag="PKM72_03150"
     CDS             14297..15541
                     /locus_tag="PKM72_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNP59809.1"
                     /translation="MITIQRGSQFSETQESHALSLVRSRSKKRAQSSYRNSQADLDMD
                     KEESSKSIAEPGSVNLESMYFRGFRSRPLLNAREELALATRLYQGTAHLRSLLQEAFE
                     LTKGLNQGPEVKLFQDELTKFSELNGYSAPVVEAIVECLGNIETFSAMNGKAGQKLSL
                     RFSEISLSIGEVRVDIEEPKDALVQRNLRLVVDVAKRYLGRGMNFLDLVQEGNIGLMK
                     AAERFDYTRGFKFSTYATWWIRQGISRAVADQSRTIRVPVHTNEASTKIAKTAQRLAQ
                     QFDREPTFEEIGQRLEMTGDRVKETIEAFQEPISLDAPSVDGETELGELIPDLACQSP
                     DEEVSRKSNAQWLNQIFQVLTDREQQVIRLRFGIGVDEAWTLEQVGRSMSVTRERIRQ
                     IEVVALKKLKESHVKAMLAEIQ"
     gene            15854..16285
                     /gene="arfB"
                     /locus_tag="PKM72_03155"
     CDS             15854..16285
                     /gene="arfB"
                     /locus_tag="PKM72_03155"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNP59810.1"
                     /translation="MPIEITPHLSIPDSDVRFSAARSAGPGGQHVNKVNSRVILEFDV
                     NHTSVLSGYQKRRIAEMVGQRMNQEGVLRLQAQRHRTQSANRADLLEKFVKILQDSLR
                     PVKARVPTRVPHRVREKRLEEKKLRTRAKRVRQNPKNIDET"
     gene            16351..18399
                     /locus_tag="PKM72_03160"
     CDS             16351..18399
                     /locus_tag="PKM72_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNP59811.1"
                     /translation="MPTPPRAKRVPYRLEHHGHVRTDEYYWLRDRENPEVLAYLQAEN
                     EYAATLRSHTRELEQTLFEEIRDRIQPTDLSVPFRLGDFWYYTRYEEGKEYALYCRKE
                     KSLANPELIMVDGNELARGHDYFSLGNWTVSFAQDILAYAIDTQGRRIYTIGFKNLTT
                     GETIDEWIPSVTGNMEWGNDNRTLFYSRQDSVTLRSNQIFCHRLGTNPQLDTLVYEET
                     DETFSVSIEKTKSKQYLLIGSHQSITNEYRYLNADNPESDFQIFQARKRGHEYDVDHL
                     GDHFWIRTNDDAKNFRLMRTETKATSATHWEEVIPHRLEVYLEGFELFKNFLVLEERR
                     QGLIHLRMLRTEDASEHELNFGEPAYLAALGDNLEADTPYLRFGYTSMTTPMTIYDYH
                     METREKILLKQEPVLGNFHISHYQTERLFAPAPDGTSIPISVVYRKGFVPDGTHPLLL
                     YGYGSYGASMDASFSSPRLSLLDRGFVYALAHIRGGEELGRQWYENGKLLSKKNTFTD
                     FIACAEFLIRQGYAAPQKLFALGGSAGGLLMGAIMNMRPDLFHGVVAQVPFVDVVTTM
                     LDPNIPLTTGEYDEWGDPNQQQFYEYMLSYSPYDNLQPQPYPHLLVTSGLHDSQVQFW
                     EPTKWVAKLRALKTDNQRLLLRTNMDAGHSGASGRFKRYEETAMIYAFLLDLAGSVDR
                     "
     gene            18646..19362
                     /locus_tag="PKM72_03165"
     CDS             18646..19362
                     /locus_tag="PKM72_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNP59812.1"
                     /translation="MTPRRFRAKVLSVRHLTQDVRELILELVDTQSLEFLPGQAIAVN
                     IPDGASGRSFLRYYSLASLPSSSHQLVLLFNAGDKGKGATFSLEHSIGEELECSGPFG
                     SFHLHEDPERDLLFIGTGTGIAPLWSMLSSLFEQSCSQPMTLLWGLRSESDIYYLDQL
                     QKWADCYKNFSFNLTLSQPAHHWRGRTGRVTHLLQEFPDVDHLAVYVCGNRKMVADVT
                     SLLQRKGICPVYRERHHEEK"
     gene            19504..20685
                     /gene="hemW"
                     /locus_tag="PKM72_03170"
     CDS             19504..20685
                     /gene="hemW"
                     /locus_tag="PKM72_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNP59813.1"
                     /translation="MNVTTQLSERSGEHDDLPADLGIYLHVPFCIKRCHFCAFYLVLH
                     EEQRVKQFLQAIEQEIALYANQPGMRERTVATVYVGGGTPTVLRPEQLALIFFSIRSK
                     FSLSEQCEITVEATPESLTDRYVDFLQQAGVTRLSIGIQSFDQQERTMLGLSGTSAEA
                     KAKVQSAKQAGFSNINLDLIYGTPNQTVQSWERTLTYALELDPSHLSCYALSIEKGTR
                     FYNEFRRGLFEVMDPDHEASFQEQAEVQLESLQWSRYELSNWAKPGFACQHNLRYWRG
                     LEYLGLGPSAQSFISECRFGNVSSLEHYSKYLMAGQLPIASKEVLSRFQQEKERIVFG
                     LRLLEGVPIDWIYRASQDECWMTSYEGLVKEDYLLPTERCVQLTRKGRQFADTVGMRL
                     L"
     gene            21241..22773
                     /locus_tag="PKM72_03175"
     CDS             21241..22773
                     /locus_tag="PKM72_03175"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNP59814.1"
                     /translation="MNPISPLPLVTGLEKQTQPVSTHRLSKSRFLAGLQCAKRLYLEI
                     HSPELATPPTPDRRAIMDMGTDVGVVARQCFPHGVLIEQTHRQIPAALLRTNEIVKDA
                     DIPSLFEGAFVWQGILVRVDILERIDARRWRLIEVKATSKVKATHLDDLAIQAAVLEG
                     AGIEVSGCFLMHLNTQYSYLGGDLNLEEMFVLENKTEEVEARRPLIQAQLEDMWKTLE
                     QATPPSIVPDNHCYQPYECPFWNHCTKGKPQRWVFHLPGSSKLQPLLLEEGIETIDEI
                     PDHIQLSATQQRVKDNVEWISPLLRPRLQSVRFPVHHLDFETFMPAIPAFAHTRPYRP
                     IPFQWSNHIEYPDGTVVHHHYLCTDGRDPREEVALSLLESVGDAGTICVYSEYERFLL
                     FGLGDVLPQLKPTLSKVVRRLWDLLSVIQQHYYHPNFHGSYSIKTVLPALVPSLAYDD
                     LTIQNGAVAAVMYQKMVFHETDLMERAHIAQALHEYCGRDTWAMVELRRVLLDRAGGC
                     SP"
     gene            22922..23374
                     /gene="aroQ"
                     /locus_tag="PKM72_03180"
     CDS             22922..23374
                     /gene="aroQ"
                     /locus_tag="PKM72_03180"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNP59815.1"
                     /translation="MNILVLHGPNLNLLGTREPTIYGTITLDEMNVSLENLGKELGVS
                     VESRQSNVEGELITWIQEAKARFEGVVFNPAAYTHTSIALRDAIVSVGIPMVEVHLSN
                     IHRRESFRRRSYLAPVSLGQISGFGPQSYLLGLRALVDALQRTIPLHH"
     gene            23414..23974
                     /gene="efp"
                     /locus_tag="PKM72_03185"
     CDS             23414..23974
                     /gene="efp"
                     /locus_tag="PKM72_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNP59816.1"
                     /translation="MITTAEFRNGSRLELDGQPYYIVEFQHVKPGKGGAFVRTKLKGY
                     KTGNVIDRTFRSGEKFDEPNLEECDMQFLYNAGEEYAFMDISSFEQFTYLKNQLGENV
                     DLLKENTVVKILLYHDEPIAVELPVFMELKVVETDPGIRGDTASGGTKLAVVETGASV
                     KVPLYLVSGEVIKIDTRTRTYVERAR"
     gene            24017..24484
                     /gene="accB"
                     /locus_tag="PKM72_03190"
     CDS             24017..24484
                     /gene="accB"
                     /locus_tag="PKM72_03190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNP59817.1"
                     /translation="MADSSRREIEDLVEVLNRHHLTELEFEKKGVRIRIRRDHVPAVT
                     SHQIDGSKPVDTGSSLEKPVPILSTEPAHFLTVTSPIVGTFYRSPSPDTDPYVEEGDI
                     VKKGQVLCIVEAMKLMNEIEAETDGKVVKILVESTTPVEFGQPLYLIDPLSGS"
     gene            24581..25930
                     /gene="accC"
                     /locus_tag="PKM72_03195"
     CDS             24581..25930
                     /gene="accC"
                     /locus_tag="PKM72_03195"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNP59818.1"
                     /translation="MFKKILIANRGEIALRVNRACRELGIRTVAIHSDVDAQSLHVRY
                     ADEHVCVGPAEGGLSYRNIPNVLSAAEITGVDAIHPGYGFLAENAHFAEVCESIGITF
                     IGPTSEHIALLSDKSKARAHMKKRGIPVLPGSDGELNDPNTCVAIAKKLGFPVIVKAS
                     AGGGGRGMRVVWHEEELAQAIESAQLEAQTAFGHGGVYLERFFEDPRHIEFQIMADNH
                     GHVVHFHERDCSIQRRYQKVLEESPSPALDDSLRRKMGQVAVEAIKSVKYRNAGTVEF
                     LMDQSGKFYFMEVNTRIQVEHPVTEMVTGVDLIKEQIRIAAGEELSYRQKDIQLTGHA
                     IECRINAECPDRFTPSPGTITKFCPPGGPGIRVDTAMDTTGIVHPYYDSMIAKLIAFG
                     RDREEAMARTRRALDEFVIEGIKTSIPLHRRILDHPDFQKGPVSTRFLDRLVALQSP"
     gene            25927..26580
                     /gene="thiE"
                     /locus_tag="PKM72_03200"
     CDS             25927..26580
                     /gene="thiE"
                     /locus_tag="PKM72_03200"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNP59819.1"
                     /translation="MSAVPISRISLPTVFSRFYLIVDDSWASRCSLAEVVQQAGEAGV
                     KLVQYRNKTGSMKQAYEAGRVLRNVAAERDMTFIVNDRCDLALALEADGVHLGQADLP
                     LLLARKVVGQKMLIGVSTHNPEQVRLATEEGADYLGFGPIFSTRTKSNHEPVVGIEGL
                     AGIRDLTPLPIIAIGGIVPDSVPALQAAGANGVAVASAILNASDRPKALARFMAIFQ"
     gene            complement(26567..27364)
                     /gene="bioD"
                     /locus_tag="PKM72_03205"
     CDS             complement(26567..27364)
                     /gene="bioD"
                     /locus_tag="PKM72_03205"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNP59820.1"
                     /translation="MESGQPDLVTRTFSSISLTMSAQSDHLAIFMTATDTGVGKTTIT
                     AALVLALQKKGHRVGVMKPVETGLDPQQPETSDTGRLQSLLSPPPPFASICLYAFPQP
                     IAPLACARAHGIAIDVAGIATALHVVRQQHSVCFVEGAGGVLTPLSPTHTIRDLIVSL
                     NLPAIVVSRTSLGSVNHLLLTLEALHGAGIKPCGIVLNDPLPSTQNECFIRQRTSTIG
                     LIHEWSTVPVFGPLEYQKTVQTDWRKGMENLANHPEIQRLANHLIEK"
     gene            complement(27337..28062)
                     /locus_tag="PKM72_03210"
     CDS             complement(27337..28062)
                     /locus_tag="PKM72_03210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP59821.1"
                     /translation="MNTRSNIMVFGGSGVIGGAIAKVFGHQGWRVGLHYHQHQTAANA
                     TAAAIRKAGGESHLYQADVANNLKIQAILQEFIQSHHSLNVLVWAVGVAPSKLLLKTS
                     PEDWTRTLHTNLTGAYTVLQAIAPIFEQQHDGAVIFVGSLSAEQGVAGQAAYAASKAG
                     LTGLMQTVAKEWGAFNIRINMVFPGWHTSPLSEPGWQSAMEPGNHTLPRPSSLQYVAT
                     TVYHLALSPNTSGQIWNLDSRIW"
     gene            28470..29297
                     /locus_tag="PKM72_03215"
     CDS             28470..29297
                     /locus_tag="PKM72_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59822.1"
                     /translation="MVYSSDSTKFIVDKSHEYFPDQVGNEWVYKGTMIEGGMVQIKNE
                     DTQFTNISKVTGTEILEGVEVTVFNDTNPGNQGPSDSYYRRDIAGIRYYGSKPGTTLE
                     RQLVPYQIVRFPLEIPTSFEQFNRTGVDFGTDVDHDGEKETVDIEATVSVQGQETVIV
                     PAGVYPEAIRLEAKMIMKVHLSESGETIQGTDTMTAWFARGVGLLKYIERQTIPSVRS
                     QNERMVEVIEELEKVTVASEVALLRRSEATAKRVFRNHFFHHELLKIPASAGFPADS"
     gene            complement(29190..32180)
                     /locus_tag="PKM72_03220"
     CDS             complement(29190..32180)
                     /locus_tag="PKM72_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNP59823.1"
                     /translation="MIAHDSLEPDIRRIGQTLAKYAHHHTPGFWDHRWWTSLLLDWGT
                     RDEQFKVQLFRFIDVLPSLQTDAQFVRILKEYFQDLPTLPGPLQWLLHRFSKNSLTAQ
                     VGTRILRRQFLKMAYTFMAGETVGHAVPTLTQLWEAGSACSLDLLGESTVSEEEADQY
                     HTRCLQTLTQLHEIIPQWNHQPLLETDHLGSLPRINLSIKLSALYSQLDPIDPESSYA
                     GVAPRLRSILELAQALPASLTFDMEQAELEPLILLVFMRLFSEPAYQHFPHASIAIQA
                     YLKHTNHTLDTLLAWGKQRNTPFGIRLVKGAYWDSEVIRYQQQGWPIPVFLKKTDTDA
                     NYEHLAQRILEHRAFIRPAFGSHNIRTLAVVQAMGDSLNLPPGTFEYQMLYGMAEPLR
                     DAVVQQGFRLRVYAPIGELLPGMAYLVRRLLENTSNESFIRRQYETAESLEHLLLAPS
                     NAHRNEEETMSREIQSEATVLAGDSPDRFSNEPHSDFSQQDVQRDFSLALNHIGPLLG
                     QIHSYPVPTPIQWFGAELISTNPSFPGQIIAKFPTPSPDQIDPIVHIAHDHIGTWAKV
                     PPKARARILFHAAGLMRQRRPELAAWEILETGKPWREADADVAEAIDFLEFYGREMIR
                     LGTPQRLGIEPGEYNQRIFQPRGLAVVIAPWNFSLAIPTGLVSAALVSGNVVLFKPSE
                     RSPMMGYHLFTLLMEAGLPEGVLQFLPGGPDVGQALVKHPKVHLIAFTGSQTVGLNII
                     REAGQVSAGQRHIKHVIAEMGGKNAIIVDETADLDEAVIGALASATGYQGQKCSACSR
                     ILVLNNVYPLFIERLKQAASSIPIGPPEHPGNRMGPLIDQRALERVRHFVQLGKTDGT
                     CLLDCQIEGPGYFQGPVILTDLPPSHPVVQEEIFGPVMVVLKVSTISEALELANNSPY
                     ALTGGIYSRSPANIQLARDTFDVGNLYINRPITGSLVNRQPFGGHRLSGIGRKAGGSG
                     YLEQFMVEKVITENTLRRGFAPTQ"
     gene            complement(32264..33196)
                     /locus_tag="PKM72_03225"
     CDS             complement(32264..33196)
                     /locus_tag="PKM72_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59824.1"
                     /translation="MKSQLLPTQLNLPAEIKEAIIIYLNSLATTIHTQTFREILIKST
                     IGIPPLSDIIPDDAHLKWITKHSPPQALESVINGYKQVSAWSHTMTSHPPPPSEEFAK
                     FASYYDQTYPDWDNSPQSWTSTFTQHGQKGIEERLLEYWQTPDHIADQHQSAQPIHDA
                     YTQHYIETRLFPMFRMILLTKISELEATAHRTAWEAWQKIQQWQKLEQANVDTTRLCG
                     TWRWIVHNHQNHGDRKMTVTFSPPDQSSPSQIAPTTIEIHGDTVYLQWTFPQGVQEDS
                     LLLSNNDTHLEGTFKNSLGPHGSISGKRLSACEP"
     gene            complement(33508..34329)
                     /locus_tag="PKM72_03230"
     CDS             complement(33508..34329)
                     /locus_tag="PKM72_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNP59825.1"
                     /translation="MTQHTTPYDIEQIGYTVVRFNSSGIFETHKDHMVDPYAETRHAK
                     GPQVEGLQFAPQEANKILPAMVLLHDRYGLTSHIQELAKGLSCQGYVVLVPNLYVRQG
                     GMVTANAEVADALMERVNEQQALQDINASFEFLNANLTEDINLERTTRNAHAVIGFGM
                     GGSLAIKTAAHRRRLQAAVAISGTLPTDPELAQRLYCPLLLQTPGKSLLNSAEERDQF
                     CQMANQAGKKIEVRSYPDASDEFWQSSTPSYRASDLEDVLQTSIDFINAIIHKTP"
BASE COUNT         8596 a         7592 c         9389 g         9338 t
ORIGIN      
        1 tcccggagtg gcgagcgaga agttgccgac gaccagttgg ttcgggccct ctgcgctgac
       61 cgaggtcgag tggatcagat ttataaggcc taaggccagt gtgagaaggt agaagaaggc
      121 gcgtcggaat gtggtgagtg tcatgcctcg tctctgaatg agtttgagag gtgttctaaa
      181 aattcgttga ctggctcggt tcgccatgag cctgtcaaaa ggccgccatg agcggagtga
      241 gcggttactg atggaattca atcttacctg gtccatgcaa agagtttgcg gaggatctgt
      301 tttttccctt ccgtcagcgt ggtttttcgc caggcatttg cggacttttt gacgacttct
      361 gcctgggttg gataggggtg aatggtgctg gctatggtgt taagtcccag gcctcccttc
      421 atggccaggg tgtattcact aatcatatca cccgcatggg aggcgacgat ggtggctccg
      481 actattttat ccgttccttt tttgacgtga actctggcga aaccttcctc ctctccatcc
      541 agaatggccc gatcgacttc attgagagga aaggtgaagg tgtcgatcgg aatgtttttg
      601 tccttcgcat ccttttcata taggccgaca tgggcaattt ccggctgggt ataggtcgcc
      661 cagggaatta ccagttggtc cgtgctcccg taaccaaggc cgaacggatg gggaaatagc
      721 gcgttctgaa tgacgatttg cgcttgggca tcggccgtat gcgtgaactt gaacggagag
      781 cagatatctc ctgccgcaaa tattttggga ttgctggttt gaagccgagg gttgaccttg
      841 actccggttt tgtcgaattc gaccccaacg gcctctagtt ccaatccctc aatgttcgga
      901 ctgcggccga cgcccaccag aatttcatca accgggatgt cgtaatgggt gccatgcgag
      961 tcaaccacaa ggtgcttggc gcctttggct gactgaatat tcaagtcctt cccgcaacaa
     1021 agcagttgca cgccgtcccg cacaatcgat tgttccacaa tctcggcagc gtcgcggtct
     1081 tcatttggaa gaatgccgtg catcgcttcc accaaaaaca cctgactgcc gaatcgggca
     1141 aatgattggg ccagttcgca gccaatggga cccgcaccga tgaccccgag tctcggagga
     1201 agttccgtca gagaaaaaat ggtttcgttg gtgagatagg ctgtgttggc caagcctgga
     1261 atcggtggag ctgtggcccg ggctcccgtg cagatgactg ctttggtaaa ttgtagtgtt
     1321 gtcccatcca catcgatcgt gcttgaactg gtgaatcggc cgttgccgat aaatacatcc
     1381 acgccaagtt tcgtaaagcg atgagctgag tcgacatgac tgatccgtgc ccgcagtttt
     1441 cgcatgcggg ccatggctgc accgaaatcc cgtttgacct ccccggaaat atgtacacca
     1501 aattgctcgg cctctctgac agcggtccag gccttggcag cgcggatgac gcccttggac
     1561 ggcacacacc ctacattcag gcagtctccg cccatcagat gtcgttcaat caacgccacc
     1621 ttggctccga gagctgacgc aatcgcggcg gtgaccaagc cggcagttcc ggccccgatg
     1681 atgacgaggt tatatcgtcc ggatggcgtg ggattgcgcc attgaggggg atgcacattg
     1741 ttgactaatt cctggttata ttgatccgcg ggaaggacta aactggtatg ggctggtgat
     1801 tgtgtggtgt tcatctgtgg cctctagttt agtaaagggt caaagtattt acgatttagt
     1861 attgatggct ccgctttgag gcttcttcca tttctgatag gccaccggca taagggccaa
     1921 caggcctagg agcgtaaagg cgagtaagac cggtggactg gcaatttcgc tcaaagagtt
     1981 aatggcgccc aattggcgac cggcaaaagc aaagacaaag ctgccgggaa tgatgccgat
     2041 ggacgtcgcc agaacatagg tgccgaggtt gacacgggtg aggccggaga ccagattcac
     2101 gagaaaaaat gggaaggcgg gaatgagtcg aagcgtcata agataactaa acgcattatt
     2161 ggcaaatcct tcctggatgg tcccgagtcg gtcgccaaac tttcgctcca cccaatcgtg
     2221 gagcagataa cgggccgcca aaaaggcgag cgtcgctcca gcggtggctc cgatgttgac
     2281 gaatagggtt cccatcagac ttccaaagag gaatcccccc gtcaacgtta agatcgctcc
     2341 accgggaagc gagaaggcgg tctggagaat atagacaaga ataaagacgg caacggctgt
     2401 ttcataatgg gaggccgtgt acgctaataa ggtgtcccgg ttcgctttta aggcctcgag
     2461 agagagatat tggccaagat caaaatataa aaaggcaccg atccctgcca gcagaatgca
     2521 cccgatgaga atctttccct tcaaggaatt gggtgcgtcg gttggcgtgg tagatggatg
     2581 ggacatgtga ggctccatta tgtgggtttt catgggaatt ctccagtcat gttttttgaa
     2641 atccggtaat tggcaaaccg ggtatcgcta aaattcctag tgtgtcaagg ctatagagat
     2701 ggttaactct atttctgttt gccatgaggt gtctttgacg atatagagtt gtagtcacga
     2761 tgttgcgcca tttgttacgg ggaaaatttc agaggaagag ttgcccgttc cattccggat
     2821 tgacgctcgt acgggctgtt tgaacagggg ggatatcttg caaatgctcg ctaacgttac
     2881 gcggaaggta attttccgac aagttcctca gccggatagg tgagaatttc agcaagcgtg
     2941 ggatggtaat gaggtatgcg gagaagatcg tgaaccgtcc catgaaaatg catgatggca
     3001 atcagttcat ggatgagttc agaagcttcc ggcccgacga tatggccacc gagaatctca
     3061 ccagactgag gatgacagag cactttcacg tgtccatacg tttcacccag gcagagagat
     3121 tttccatggt cgttaaaggg gtaggaagcg acaagatagg ggagttgacg gtcaaggcat
     3181 tcccgctcgg acagaccgac gctggccacc tggggatcag taaagacaac ttgggtttta
     3241 agacggtcag aaaaatgttt gggaggtaga ttcgcatgaa cggcgttcca tcctgcgatt
     3301 tcgccctctt cgatggcgat atggacgatt tcatagccgc cgttgacatc tccaacggca
     3361 aaaatatgcg gctgggatgt tctcatttcc ttgttcacta aaatggcgtt gggttgatgg
     3421 gcaatctgag ccgcgtccaa attgagtccg tcgatattgg ggcggcggcc tagagcatgg
     3481 aggattgacg tggcggtgac gtggcggggt tgaccttgat gaagaaagtg aaccgttttc
     3541 atttctccag atgcctggac ctgttggagg caggtattgg tatagactgt cattccttca
     3601 gcccgaagat gagcttcaac tggttgaacg agatcttcgt ctgtgtcgga gagaatatgg
     3661 gtgcttcgtt gaatcaaggt tactgcgact ccgatgcggg aaaaaaactg ggcgagttcc
     3721 aacgcgaccg gtccgccgcc cagaacaatc atggattcag gctgtgaatg aagctcgagt
     3781 gcctcatcac tcgtgagata gccggcttct tctaatcctg gaatgtcggg gtgcgatacc
     3841 acggatccag tggcgatgat ataggctttt gccgatagct gctgttgacc gacttgaatg
     3901 atcgttggcg aaacaaagtg ggcatattct tgatagagtg taaatcgcgg atggtggagt
     3961 gcttggatgc gatcttcagc aaattcctta attaacttat cttttcgatc aataatctct
     4021 ggcagattca cctggagcct ggacgaggta agaccgaatt ccggcgcacg acggatgagc
     4081 gccgcgaggt cggaggagcg gagaatggct ttggtcggca tgcatccccg taaaatgcag
     4141 agaccgccta acggaccctg atcgacgatg ccgacgtctg ccccttccgc ttgggcggtg
     4201 cgtgctgctg catagcccgc cgaccctcct ccaattatga tgatgtcatg ggaatggatc
     4261 atgtggagtg cctgtttttc cggatgaata gagagaaaac atggtcgaat tacgaatgca
     4321 agtatgtagc atgaattcat ctcacgaaga aaggcagtcg tgctggcggc cagtgcagga
     4381 tggttgaagt tgggtggctg tgtacccggg tctattcctc cggcttccct attcatggct
     4441 accgacttga atctgaccgc attgattggg aacaggctgg ccaatggatg aaagtctttg
     4501 catcgtttga cgcagcttct agattattcc tcaatttgtg gaggcccact ttaatgcggg
     4561 actggcgttg caccagaggg gtcgacataa ggaggcgacc cggcgctttc gacgagcagg
     4621 ggcgttggag tttcggaatg cggcggttat tcactccacg ttgtatcgga atcatctggg
     4681 actgtcatcg accctcgagc gctacctgaa cggaggctat cgttattagc agtattgcct
     4741 ggtagaagat ggaaggtatg tttctatcgg ccagggttgt tggaagatgt cgtctgtttt
     4801 tgcctgattt cttcgacttt ggcgatgagt gtttccactt gatagggttt ttgaataaat
     4861 gcagccatgt tcgggttgtg aaatcttttc atcgcttctt cctcggaata tccacttgat
     4921 aaaatgaccg gaatcgttgc gttaagtcct tgaatgtgtt cgaggaattg ttgaccgtcc
     4981 atattgggca tggtgagatc taagagtacg accataattt cctgctgatg ttgttggaag
     5041 acttgaatgc caatttgtcc atccgaggcg actaaagttt gtaatcccat ttcttcaaag
     5101 atcatagaac aggctacgcg aacgtcctct tcgtcatcca ccaccaacac catgggtttt
     5161 ttggtcggtg aggcatgttg ggttggaatg gagaagaccg gccccttctt tgttgaagga
     5221 ttgggtgtgg tggcgggaaa cagtaacgta aattctgtcc cgatatctgg ttgacttcgg
     5281 acggcaatgg aggcggcatg ggcctgggcg agtccaagaa gcgctgcgag tcctagccct
     5341 cgtccaggaa gtttggtcga aaagaatggg tcaaaaattt ttggaattgt gtcggacccc
     5401 attccgcacc cggtatcctg aatggttagg gcaacacccc ggctatgcgg gagctcacca
     5461 ataacatacc aactctgaaa attctctgtt gccggctccc attcgaatgt acggatggtg
     5521 atgaccccat ccccttctcc aaaggattct gatgcgttta ccacgatatt cattatgatt
     5581 tggcgcaatt gagcctggtc ggctcgaatc ggtggaaggg agggggcgag atcgtattcg
     5641 aacgtgagtc gttttgaaag ggtggcccgg agaaaagggg ccatgtcctc gagaagattt
     5701 ggaagattga taacttgaaa atccaagcgg gtctgtccgg caaatgccaa catttgattg
     5761 gccaattccc caccgcgaag accggctttt tcaatgaagt ccaagtgctc tcgagccgga
     5821 gcgtcaggag gaagggagcg aagtgcgagg ccggagcggg ccacaatagt catcaggaga
     5881 ttattgaaat catgcgcgat ccctccggcg agtacaccca ggctttccag cttttgtgaa
     5941 tattgccgtt cgatttcatg acggcgtcgt tcgtcttcgg cccttcgctg ttctgtcaca
     6001 tccctgagag aggccaaaaa ggcgggttct ccatcccact caatagggac cactcgcatt
     6061 tctacaggag ttggtcgacc gagttcgctc atgatttcta tttccatggt ctggtttgca
     6121 gagaccggga aaccgaatgg ggatccgttg agttgttcgg atgtcttttg aaagagctgt
     6181 tctgctgcag gattggaaaa acggatcagg ccgtcatgtc cgacgacaag gattccgtcg
     6241 ctggatgatt gcaagatgat gctggactga cgttcaagag agcgtatgcg aggtatggtg
     6301 acacactgct caatcgcctg ctccatatga tgccagagga atttttccga tagagtgttt
     6361 ttgttgaaat actcttgtgc gccaagtttg attgcgtgag ccgcaatttt ttcgtcaccg
     6421 tacccggtga gaaaaatgat gggattcttt gactgcgact gtgaaccccg tactttcgcc
     6481 aggaattcta atccggtcat atccggcaat tggtcatcca taagcagaca gtgagggagg
     6541 gagatcttgc aggcttccca ggcctggact cctgtttcca tttcttgaat atcgaggcga
     6601 aacggggctg tgcgccgaat ccaacgagta taggttgatc ggtccccagg gctatcgtcg
     6661 acgataaaaa ttcggagagt ggtgagttga gatttttcaa tacggattgg catgtttgac
     6721 ggagatccca ttaatggaag tggaaagacc gggaatgagc tgggatgaaa aaatgtattc
     6781 gtcaagcatt aggaaggccc tgcatcacct cgaaattttc cagtttgtgt cttttgtcca
     6841 gtcttaggtg gagaactaat gtaaaccggt ataattatag ttttatcatg gcttgtagtg
     6901 gcaatcggtt tcaaccggtt tcacaatata actgttgatc tctacctgat tccaggctta
     6961 cctatcgcat ttgtctggta agaggtttcc acgatgggtt gaatcatttt gagtttcaaa
     7021 tcagactgtg cttttggacc cgtttcccgt ttgtcggtac ctggggatat tcagatcgag
     7081 aagaatcgta cctggtggag gatcttcggt attatctgcc tgattatttg gacaaatagg
     7141 gccagaactt attcccattt tcgccatgta tgacggagtc ctggttcttg aaagctgcga
     7201 atggtgtctt ctgcatggtc ctggctggct tttacccgaa gtatggattg gctctgagca
     7261 gaaaggttag tcattcgtgg tccgattagc cagacctctt gaactcttac gaaggcacag
     7321 gatggcatca aaatctacat ggatgaaggc taaatccgta tggccaactc ctaaatctta
     7381 ccttagcgat tcggtcagtc ttctcatccg tgggatctaa tatattgtcg tatgggattg
     7441 ccccttgctt tcttcgtggc gccgggctca acggtgggcg atccggaact tctttgccga
     7501 tccgcatagc tcagggcgcg tgagaccaat gaaaatatcc atggcctgcg gcggcagtat
     7561 tgatccagca cgaatttgtt caactgtacg cagtgcgaac tcaatcccat tgcgtagcgt
     7621 ttgaacacca gtttcagaaa atgtctgaat tgggcaatgc tccagaaact gttcacaact
     7681 gcggtatcag tcacccgtgg cacttgtagg agcttgaagc agcccctaat tacatatgca
     7741 ccatgtgctg ggcgctttca ttggcgggga gtggcggggc gatccaaaat ccttgcattg
     7801 catcgcatcc atgttcttcc aggaaatccg cttgttcttg cctttctact ccctcggcga
     7861 tgacttgtag tcctagggct ttggccaagg aaatgacggc caaaacgatc gcggcatcgt
     7921 tggtactgta tgggagattt tgaataaggc tttgatcgag tttgatgcca tcaaggggaa
     7981 atgacttcag atatcgaaga gaagcatagc ccgctccaaa atcatcaatg tacagatgga
     8041 cgcccatcgc tctgatttcg cggagggtct ggatggtttc ttccgtttca tgaatcagaa
     8101 acgtctcggt cagttccagt ttgagatttt ccggatccag atgcgaatgc ttgaggatgt
     8161 cttgtatgag ttcggggaga tgcccctgat ggatttgcct ggctgagagg tttacggcga
     8221 catgaggagc aggaaatccc tgcttttccc aatatttggc ttgtttgctg gctgaacgta
     8281 aaacccattc ccctatagga atgatcatgc cattttcttc ggcttgggga atgaagtgat
     8341 tcgggccaat aagcccttgg taggggtgat gccaacgcag caaagcttcc atgccaatga
     8401 cttgcttggt gcaaatattc atttgtggtt ggtaatggag gagaaattct tcttttgcca
     8461 acgcccgact gagctccatc tccagtgtgg agccatcaag gccagctgtt ttcatgccgg
     8521 cggtgtaaaa ctgataggta ttgccgcctt gggctttggc tcgatacatc gcggcatcgg
     8581 catgagataa aagttcctgg gggttggcgc tatcccaggg atagatggtg atcccgaggc
     8641 ttccggtcac acgcagttgg tgctgcttga taatcacggg ctgggccagg aggtgaagaa
     8701 tgcgatgtgc aacagcggcc acgtcctcaa tcgacacgat ttgttccaaa aggactgcaa
     8761 attcatctcc tcccaatcgg acacccgtat caatttctct aatacatttt ttgattcgtg
     8821 cggccacagt tttgagtaac gaatccccgt attcatggcc gagggtatcg ttaatatctt
     8881 tgaaatgatc caggtccaaa aacattagag cgattgccgt gtcgttccga tggcagcggg
     8941 ccacggcaca atttaatcgt tcataaaaaa ggcctctgtt tgcaagccct gtcaggtggt
     9001 cgtaatgagc caattgggta atgcgttgtg tggccttctg gcgttccatg gcgtaccgaa
     9061 tggcccggca cagagtcgag ctgctggtct ggcctttgat taaatagtcc tgggctccac
     9121 tttttacggc ttgaatcgcc tgggcttctt cctcaactcc gctcaaaatc agaatcggga
     9181 tatcggggca aaactgcatg aattgcagaa tggattcaaa tgatttcccg tctgggagat
     9241 gcgagtccag aataatggct tggaatttgt tttgttgaat gaggtcgaga gcatcactga
     9301 gagaaaggct attctccaaa gtaaattggt tttggaggcc ttctttcagc atgtttctaa
     9361 taaccaacgc ttctttaagg tcatcttcaa ccaacaaaag tgaaatcatg gttgctgctt
     9421 tcttcctgtt taagctcgtt cctaagtcca ggaactattt aggttcgaga aggaggtcat
     9481 aggttatctt gtcggatgta gcagtgtttt cttaatagtt tttgtagatt ttgtcttttt
     9541 gaggagaaga tcacaaaatc aatttcagga tcttaatgct aaaattttaa ataaaaacaa
     9601 gcggttaatt ggtttaatag gtcaaatgaa ggcgacggat tttcagagaa atgatacgtg
     9661 ctttgttgta ggtgactttg gttgattctt aggaaattta tctttacttt tcagacaaaa
     9721 tttttatata gtgttatcaa attgcgcctt gtatttttac cttcgctttt tgtaaacaat
     9781 aggtcttttt cctggtttcg cccaaaactc ggtatgacgt acttaataca gattggaggg
     9841 cgatttatct ttaaatctgt atgagagatt gctcactttt ctggaagtaa gattcttttt
     9901 tggaagaacg gtgaggaagg actgattggt ttcttgtgct gcgagggccc gttggctcaa
     9961 tacgaaaagt ggtatgattg ggttgagcta ttaattttta aggagctatg ttaatgggga
    10021 agacagcgtc attgccggag gtgattcagc gtttaattga gattggtaag cccaaagggc
    10081 atgtgaccat aaaagacttg acgagtattc ttcctgccga ccagatcact tctgatcaat
    10141 tacataccat tttaagcgag ttgcacgaag tcaatctcca ggtaatcgat ccttcaaagc
    10201 ggacggcctt gcagttggcg gcgttaaaaa agaatgcggg gcaagaggaa tcagatgatt
    10261 cagaggagga aacggattct tccctggata ttgatttgac gccaggtgaa cctacccgca
    10321 tcgatgatcc ggtccggttg tatctaaaag aaatgggacg ggtgcctttg ctcacccgtg
    10381 aaggcgaaat tgagctggca aaacacattg aggaaggcaa acgtgagtta gcatgtgctg
    10441 tctatggcat gccgttgaat atcaattacc tagaatctct ccacgaccaa ctgaagctgg
    10501 aggagattcg tgttcgagat atcgtgcttc tgcaggaaac cttagaggaa gaggaagttg
    10561 aagaggaggc cattgccagc gaacaggagg atgaagagct ccgattacga acgttagaga
    10621 gtctggcaac ggttcgaaag cttgggcgtg ggttgctttc actttacatt caaaatcgag
    10681 atgggcaaac gactaaaaat tcgaaggcgc aacttgagaa gcgccggatt ggtctggctg
    10741 accaatttgt ggatcaaatc gaggcactta atctgcatca gcgtgttaaa gaccaaatgt
    10801 taaggcgtgt caaagatgtt gggcaggaaa ttcgatcggt tgaaatcatg actgcccgtc
    10861 atacgaaaag gcttgggctt gcagggcagg atggtatgca ggtgattggg aaaaaaccac
    10921 gcgcgaccac aaaagggagt tcgcataggc gaaaacagca attaactccg gaagaaatcg
    10981 aaaagttaaa acaagaagtg gaagcaggga aaggcaccct cttacgcatc caggacgagg
    11041 tgctgggtat gccgatggaa gaatttacgg ctgccctgac tattctggag accgccgagg
    11101 aaaaggtgaa acgagggaag gcccaattga tcgaggccaa tttgcgattg gtcgtcagta
    11161 ttgcacgaaa atatacaaat cggggattgc agtttatcga tttgatccaa gaaggcaata
    11221 ttggattaat gcgtgctgtt gataagtttg agtatcagcg tggctataaa tttagcacct
    11281 atgcaacgtg gtggattcga cagggtgtga cgcgtgccat agccgatcag gcccgtacca
    11341 ttcgtattcc ggtgcatatg attgaggcca atacaaaact agcccggacg gcaaggcagt
    11401 tggtgcaaca attagggcgt gagcctaccc cagaggaaat tgccgaacgg atggggctaa
    11461 ctcctgaaaa ggtccgcatg atgttggata tttctaaggg gactatttcc cttgaaacgc
    11521 caatcggcga aaaagaggat agccaattag gggacctaat tgaagataaa aatgcagttt
    11581 ctcctatgga tgctgcaaac cgttatgatc tgcaacgtca gattgctaat gccttggggg
    11641 tgttgactcc tcgagaagaa actgtgattc gtaagcggtt tgggatcggt gatagtaccg
    11701 atcataccct ggaggaaatt ggtcaggatt ttgacgtgac tcgtgagcgc atccgtcaga
    11761 ttgaggccaa agccctaaag aaacttcgtc acccaagttg cagccataaa cttcggagtt
    11821 tggtggacca tctctagtct gagtctataa atgaatcgat aaggccccgt catgtacatg
    11881 acggggcttt tttttgccat atccctaggg aattatgtta tggatacttg tgattctctc
    11941 ttttcatgaa tttctattat tcattccttt gacgttcccc ttctccgttc cggtatgtag
    12001 atggtaagat ttttttaaga tgcggatttt agtggtggaa gatgaaccta aggtggcttc
    12061 ctttatccgg cgagcgctgg aggaagaaag ttatgccgtc gatgtatgcg cggatgggcc
    12121 gggagggttg gattgggctc aagccgtaaa ttatgacttg attgtcttgg atttaatgtt
    12181 gccaggattg ccaggtctcg aagtgcttaa gcgtgctcgt gccgcaggtg tcaaagctcc
    12241 cgtacttatt ttaacggcac gttcagaagt ggaccaacgg gtcaaggggc tggatgctgg
    12301 ggctgacgat tatttaacga aaccctttgc gattgaagaa ttgctcgcca gagctcgagc
    12361 gctccttcgt cgagcaggag gggctccaac cggaattctt caagtagatg atgtgatact
    12421 aaatcctgtg actcgagagg tgacacgtgc cggccaacgt cttgagttta cgactaaaga
    12481 atatgcgctc ttagagtatt taatgcgaaa tgcgggacgg gtgttaacgc ggtccatgat
    12541 tacggaacat gtgtgggatt tggattttga tacgtttaca aatgttattg atgtgtatat
    12601 cagctacttg cgaaacaaaa ttgataaagg acgggaacga agtttgattc aaacggtcag
    12661 ggggagcgga tatatgatga ggtcggacgg gtgaaaacga cctcgattcg agttcgatta
    12721 actttatggt atggatccgc gttggctctc attctggttc tttttgccgt ggcattatat
    12781 ttggtgatgt cccgcgcact tcgggaacag gtggatgcct cgttggatga ggccgcagct
    12841 gtggcgattc gaaccctcgg agaacaccgg tttggtccat tcttgatttt tgaagattta
    12901 tctcaggact ttcctgaaat cgcgcttttg gataagtttt ttcaaatttt tgggccggcg
    12961 gggcaggtga cgattcagtc agctaatatt caaagccgag agattccatt gagtcagaca
    13021 tcgtttcgtg cctctcttga tggaaaatcc acgtttgaat ccgttcaatt tgaaaaaggt
    13081 gttccactgc gattgttgtc ggttcctatt cgtcaagatg atcaattggt gaatatttta
    13141 cgcgttggaa catcccttta cccaacagag cgaatgctgc atcggctctt agccgggctc
    13201 tacatcgcgt ctcctttagc cttgctcgtc tccttggtcg gagggtggtt tttggcaggc
    13261 cgagcgttgc gtccggttca tgccattacg caagcggccc aacggatagc agccggggat
    13321 tggacccagc ggattcaaac tcctcattcc aacgatgaaa ttggacaatt agcctcgacc
    13381 tttaatgaca tgattggacg attagaagtg tcattccggc aaatccggca gtttagtgcg
    13441 gatgcgtctc atgaacttcg aacaccctta accattacaa aaggggaaac tgagctggcg
    13501 ttacggcgac cacggcaagc agaagattat caggctgtgc tggagagcaa tttggaggaa
    13561 atcgaccgca tgagccggat cgtggatgag cttttgtttt tatcacgagc ggatcttggg
    13621 gaaattaagc tcaagatgta tccagttcaa ttggatgatt tagtgcgaga aatccagcaa
    13681 caggccttgg tgttaggcca agaacgcaat attcacacgg tactcaaagc cattgagccc
    13741 gtcgttgtcc agggagacga tctacgtttg agggaattac ttctaaattt ggtcgataat
    13801 gccgtgaaat attctcatca aggacaaacc gtggaacttt ctttgcttgt cgccggaaac
    13861 aagggaaagc tagtggttcg agaccacggt attggtcttg cttctgagga tcacggcaga
    13921 atttttgatc gattttaccg gaccgatgaa gcgcgttctc attcggcaaa aggaactgga
    13981 ttgggtttag cgatttgtaa gtggattgtg gaagttcacc atgggactat tgaggtccaa
    14041 agtgtcgttc atggtggttc atgctttacg gttttcctcc ctttggctcc gtctgctgcg
    14101 atagtgtaag acacagactt cccgcactat taaaattttt taattttccc ctaatttatc
    14161 tttcatctcc tggtggtaca catatacctg tactgaccaa atataaaagg agcctctaca
    14221 ggccaggtct acagaggtaa atttgtattg gtcaaggttt gttgccttaa ccctgaaatg
    14281 tccagagggg gaactcatga ttacaattca aaggggttca caattcagcg aaacgcagga
    14341 aagtcacgca ctgtccttgg ttaggagccg atctaagaaa cgggctcaat caagttatcg
    14401 aaattcgcag gcggatttgg atatggataa agaggagagc tccaaatcca ttgctgagcc
    14461 tggatccgta aatttagagt ccatgtactt tcgtgggttt cgctcacgcc cacttttgaa
    14521 tgccagggag gagttagcgc tggctaccag actatatcag ggtacggccc accttcgttc
    14581 attgttacag gaggcctttg agcttaccaa gggactgaat caaggtccgg aggtaaagtt
    14641 gtttcaggat gaattgacaa aattctcaga gctaaatgga tattcagctc cggtagtgga
    14701 agccatcgtg gagtgtttag gcaacattga gacattctcg gcgatgaatg gcaaggcagg
    14761 ccaaaagctt agtctgcggt tttcggaaat tagtctttcg attggtgaag ttcgggtgga
    14821 tattgaagaa ccaaaagatg ccttggtgca acgcaatctt cgtctggtag tggatgtcgc
    14881 taagcgttac ttagggcgag ggatgaactt tcttgacctt gtgcaagaag ggaatattgg
    14941 actgatgaaa gcggcggaac gatttgatta cacccgtggg tttaaattca gtacctatgc
    15001 gacgtggtgg attcgccagg gcatttcgcg agcggttgcc gatcaaagcc ggacgattcg
    15061 tgtgccggtg catacgaatg aggcatccac caaaattgcc aagactgctc agcgtcttgc
    15121 gcagcaattt gatagagagc ccacgtttga agaaattggc caacgcctgg agatgaccgg
    15181 cgatcgggtg aaagaaacga ttgaagcgtt tcaggagccg atatccctag atgctccttc
    15241 tgtggatgga gagacggaat taggagaatt aattcctgac ctggcatgcc agtctccgga
    15301 cgaagaagtg tcccgtaaat caaatgcaca gtggctgaat caaatttttc aggttttgac
    15361 tgatcgggag caacaagtca ttcgtttacg ctttgggatt ggagtggatg aagcttggac
    15421 tctggagcag gtcgggcggt ccatgtccgt aactcgtgag cggattcgtc aaatcgaggt
    15481 tgtggcattg aaaaagctca aggagtccca tgtcaaggcc atgttggctg aaattcagtg
    15541 acctgtttcg gtcgcgaaat tttaattgta caggataaaa tccatgacat cagattgaca
    15601 taggtaccaa aaaagtggtg aattcagaaa ggcaggaatc ctatggttcc tgcttttctt
    15661 tttgtgagtg tggtcattct ccttattgat ccgccaagat ggttaaagga tgacatcatt
    15721 ccgtggggaa ttacttagtg caagggagat ggaagccgtc tagtttagcg tgaggtctca
    15781 ggttggtgag tgggaaatga tttctaacgt gagcacaaaa aaagaccaga tccgattttg
    15841 aacggataac agtatgccaa ttgagatcac tccccatctt tctattcctg attctgacgt
    15901 gcgattttca gctgctcgaa gcgcaggtcc gggtgggcag catgttaata aggtgaatag
    15961 tcgggtcatt ttagagtttg acgtcaacca tacgtccgtt cttagtggct atcaaaaacg
    16021 acgaattgcc gaaatggttg ggcaacgcat gaaccaagaa ggggttctga ggctgcaggc
    16081 tcaacgacat cgcacccaat cggctaatcg cgctgattta ttggagaagt ttgtgaaaat
    16141 actgcaggat tccctgcgtc ctgtaaaggc ccgggttcca acccgagtgc cgcatcgagt
    16201 gcgtgagaag cgtttggaag agaagaaact gcgaactcgc gcgaagcggg tgcgacaaaa
    16261 cccaaaaaat atagatgaaa cgtaacgttc tgcaattttt gacgacaagg gaatttcatg
    16321 acgacccgtt cctcccattc tagttctttc atgccaactc caccccgtgc taaacgcgtc
    16381 ccgtaccggt tagaacatca tgggcacgtt cgaacagacg agtattattg gctcagagat
    16441 cgtgagaacc ctgaagtcct ggcctatttg caggctgaga atgagtatgc cgccacgttg
    16501 aggagccaca ccagagaatt ggagcagacc ctgtttgaag agatcaggga ccgcattcag
    16561 ccaacggatt tatctgttcc gtttcggctg ggagactttt ggtattacac tcgctatgaa
    16621 gagggaaagg aatatgccct gtactgccgg aaagagaaat ccttagcgaa tccggaactc
    16681 atcatggtcg atggcaatga attggctcgg ggacatgatt atttctcatt ggggaattgg
    16741 acggtaagtt ttgcccaaga tattctcgcc tatgccattg atacgcaagg tcggcggata
    16801 tataccattg gctttaaaaa tctgacaacg ggcgagacga ttgatgaatg gattccttcg
    16861 gtgacaggaa atatggagtg gggcaatgac aaccgtactc tcttttattc cagacaggat
    16921 tctgtaacct tgcgatccaa tcagattttt tgtcatcggt tggggacgaa cccccaactg
    16981 gatacgttgg tgtatgagga aacggatgaa accttttctg tttctatcga aaaaacaaaa
    17041 tccaaacagt atttgttgat tggatcccat caatccataa cgaatgagta ccggtatctg
    17101 aatgcggata atcctgaaag cgattttcag atatttcagg ccaggaaacg agggcatgaa
    17161 tacgatgtgg atcatttggg ggatcatttc tggattcgaa cgaatgatga tgccaaaaac
    17221 tttcgtttga tgcggactga aacaaaagcc acgagcgcca cacactggga agaagtgatt
    17281 cctcaccggc tggaggtgta tttggaagga tttgagctat tcaagaattt cctggtgtta
    17341 gaagaacgga ggcagggtct catccatctt cgtatgcttc ggaccgaaga tgcctctgaa
    17401 catgaactga attttggaga gccggcctac cttgcagcgt tgggggataa tttagaagcc
    17461 gatactcctt atctacgatt tgggtataca tccatgacca cccccatgac catctatgat
    17521 tatcacatgg aaacacgaga aaaaattctt ctcaaacaag agccggttct tgggaatttt
    17581 cacatcagcc actaccagac ggaacgcctt tttgctccag caccagatgg tacctctatt
    17641 cctatatccg tggtctaccg aaaaggtttt gtcccggatg gcacgcatcc cctgttgttg
    17701 tatggctatg gttcctatgg tgcgagcatg gatgcaagtt ttagctcccc ccgactcagc
    17761 ttgttggatc gtgggtttgt ctatgcgtta gcccacattc gaggtggaga ggaactgggc
    17821 cgacagtggt atgaaaacgg caagttatta tccaagaaaa ataccttcac ggattttatc
    17881 gcctgtgccg aatttttaat tcggcaaggg tacgctgccc ctcagaaatt attcgctctt
    17941 ggtggaagcg ctggtggttt gttgatgggg gcgattatga atatgcggcc tgatttgttt
    18001 catggtgtag tggcacaggt tccttttgtg gatgtggtga ccacgatgtt agatcccaat
    18061 atccctctga cgacgggaga atatgatgaa tggggtgatc ccaatcaaca acagttttac
    18121 gagtatatgc tttcctattc accttacgat aaccttcaac cccagccgta tcctcacctt
    18181 ttagtgactt cagggttgca tgactcacaa gtgcaatttt gggagccgac caaatgggtt
    18241 gcaaaactgc gtgcgctgaa gaccgataat caacgccttt tgctcagaac caatatggat
    18301 gcaggccata gtggagcctc aggccgtttt aaacggtatg aggaaaccgc gatgatctat
    18361 gcattcttat tggatctcgc cggttccgtt gaccggtaag gtattccctt ttttttcagg
    18421 aatctcattg actcgcacat tcgtcttcag ctccaggcca catttcccct agtgagtcaa
    18481 ggcgagcgtg aggaggctcc cagtgttggt tcccctgtcc tgatgggtag ctgtcctcag
    18541 agcctggctc tatccgcgta gggtctcttc agcccttggt ggaaatggaa aaacctcgat
    18601 cctaggctct tgccagatga accaagattc tctacattct taggcatgac ccccagacga
    18661 ttccgggcca aggttctctc tgttcggcat ttgactcaag atgttcgaga attaatcttg
    18721 gagttggtgg atacccagag cttagaattt ctcccaggcc aagcaattgc cgtaaacatc
    18781 cctgatgggg cttcgggccg ttcattcctg agatactatt cgctggcttc acttcccagt
    18841 tcctctcatc agctcgtact actctttaat gccggagaca aaggaaaagg cgcaacgttt
    18901 tcgttggagc attcgatcgg tgaggaactt gaatgttccg gtcctttcgg ttcatttcac
    18961 cttcatgagg accctgaacg agatctgtta tttattggga cgggaaccgg catcgcgcct
    19021 ttatggtcta tgctgtccag tctctttgag caatcgtgtt ctcagcccat gaccttgctg
    19081 tggggacttc ggagtgagtc ggatatctac tatttggacc agttgcaaaa gtgggccgat
    19141 tgctataaaa atttttcctt taatctgacc ctgtctcaac ctgctcatca ttggagggga
    19201 aggacgggac gggtgacaca tctgcttcag gagttcccgg atgtggatca tctggcggta
    19261 tatgtgtgtg gtaatcgaaa aatggttgcg gatgtcacca gtctccttca gcgtaaggga
    19321 atatgtcctg tctatcgcga acggcaccat gaagagaaat aatacgttgg tcttcgattg
    19381 gcagatttgt ctcttcatgg tgccgtttag ttccttgctt cgcacgtcca tgcaggttca
    19441 ctctcttctc gccaaaggct ttctggagga ataggttgaa aggtcgttgt gatgcaggat
    19501 gagatgaatg ttacgacaca gttaagtgag cgctcagggg aacacgatga tctccctgct
    19561 gatcttggga tttatctcca tgttcctttt tgtataaaac gatgtcattt ttgtgcattt
    19621 tatctcgtcc tgcatgaaga acaacgggtg aaacagtttc ttcaggccat tgaacaagaa
    19681 atcgcgctgt atgctaacca accgggaatg agagagcgaa cggttgcaac cgtctatgtc
    19741 gggggtggga ctcccacggt cttacgtcct gaacagttgg ctcttatttt ttttagcatt
    19801 aggtccaagt tttccctctc ggaacagtgt gaaatcaccg tcgaagcaac accggaatct
    19861 cttacggaca ggtatgttga ttttcttcag caagcaggcg tgacacgttt aagtataggg
    19921 atccaatctt ttgatcaaca ggaacggacg atgttgggac tttcagggac cagtgcggag
    19981 gctaaagcca aggttcaaag cgcaaaacag gctggtttct ccaatattaa tcttgacctc
    20041 atctacggga ctcccaacca aactgtccag tcatgggaaa ggactctgac ttatgcactg
    20101 gagctggatc cctctcattt gtcctgttat gcactttcta ttgaaaaagg gacacgattc
    20161 tataatgaat ttcggcgcgg gctatttgag gtgatggacc cagatcatga ggcgtctttt
    20221 caggagcagg ctgaagtcca attggagtcg ttgcaatggt ctcgctatga actttccaat
    20281 tgggcgaagc caggatttgc ctgtcagcat aatcttcgat attggcgagg gttggagtat
    20341 ttgggattgg gtccaagtgc ccaatccttt atttccgaat gccggtttgg aaacgtgtcc
    20401 agtcttgagc attattcgaa atatttgatg gccggacaac tgccgattgc ctcaaaagag
    20461 gtgctgtctc gttttcagca agaaaaggaa cgtattgtgt ttggattgcg tctgcttgag
    20521 ggggtgccaa ttgattggat ctatcgggca tctcaggacg aatgctggat gacatcgtat
    20581 gagggactgg tgaaagaaga ttatcttctt ccgacagaaa gatgtgtgca gttaacgcgg
    20641 aagggtcgcc agttcgccga tacggttggt atgcgattac tgtaaaaatt atgtgacttg
    20701 aattgaattc ttgaagagat gtcaatggca tttaaggaaa gtgcctgtta ttgaggcgtt
    20761 ttgctgagag aggcgtacaa tgaggatttg attgcgttat atacagggtt attcagaaat
    20821 cttccccagc ttttgtggat aaaacatctt ctggtctttt ttgaatagta aggagttgaa
    20881 tgtattttgt aggtaagatt atacggaatc tgtgttgaaa aaaatggtaa agtttttaac
    20941 aaacaattag ttaagcggtg gtaggatgtt tcgtaagtca ttggcaattt tagaggaatc
    21001 gtttctgaca gctggattgt tggtgttttt ccacaggcca tggggataaa tggaatccac
    21061 ctgaattatt cctgagaaat gtactgagga tgatgaaatt gcaggtatgt tgcatgggag
    21121 aaaaaaattg agcagtatgc ttctatctga aatgcagcaa tcatgtatgg gtcctgatgc
    21181 ttaagaatga ctttttgaaa ttgggtgttc ctcttcattc ccgggagctc tcataagccc
    21241 atgaacccaa tttctccttt gccccttgtt acaggattag aaaaacaaac ccaacctgtt
    21301 tccacgcatc gtttatcaaa aagccgcttc ctggcgggtt tgcaatgtgc caagcggttg
    21361 tatttggaaa tccattcgcc agaattagcc accccgccta cgccggatcg ccgggccatc
    21421 atggatatgg gcacagatgt tggcgtcgtt gcgcgtcaat gttttcccca cggtgttctg
    21481 attgaacaaa cacaccgtca aattcctgca gctcttcttc gcaccaatga gattgtgaag
    21541 gatgcagaca tcccgtccct gtttgaaggg gcgtttgtct ggcaaggtat tttggttcgt
    21601 gttgacatct tggagcggat tgatgctagg cgttggcgat tgattgaagt gaaggcgact
    21661 tcgaaagtga aagctacgca tttggacgat ctggcaattc aagctgcagt cctggaggga
    21721 gcgggcatcg aagtcagcgg gtgtttttta atgcatctga atacccagta ttcttatctt
    21781 ggcggagatc taaatttaga agaaatgttt gttttggaaa ataagaccga agaagtggag
    21841 gcgcgtcggc cattgattca ggcacagctt gaggatatgt ggaagacgtt ggagcaggcg
    21901 acgccaccct ccattgtccc tgataatcat tgctatcagc cgtatgaatg ccctttctgg
    21961 aaccattgca cgaaagggaa gcctcagcgg tgggtgtttc atctccctgg cagctcgaaa
    22021 ttgcaaccgc tcctcctgga ggaggggatt gaaaccattg atgagattcc agaccatatt
    22081 cagttgtccg caacccagca acgagtgaag gataatgtgg aatggatctc acctctcctt
    22141 cggcctcgtc tgcaatcagt caggtttccg gttcatcatt tagattttga aacttttatg
    22201 ccggcgatcc cggcgtttgc tcatactcgt ccctatcggc cgattccctt ccagtggtca
    22261 aaccatattg aataccctga tggcacagtc gttcaccatc attatctgtg caccgacggc
    22321 cgggaccctc gagaagaagt ggctttgagt ctgttagaga gcgtagggga tgccggaact
    22381 atttgtgtct attcggaata tgagcgtttt ctgctttttg ggctgggaga tgtgctgcct
    22441 cagttgaagc ccaccctatc gaaggtggtt cgccggttat gggatcttct ttcggtcatt
    22501 cagcaacatt attatcatcc gaactttcac ggttcctact ccattaaaac cgtcttgcca
    22561 gctttggttc cttccttggc ttacgatgat ttaaccatcc aaaatggggc cgtggctgcc
    22621 gtcatgtacc agaaaatggt ttttcatgaa acagacctca tggagcgtgc tcacatcgcc
    22681 caggcattac atgaatattg tggccgggac acatgggcaa tggttgagct gagacgggta
    22741 ctcctggatc gtgccggagg atgctcacct tgagaagtgc gctaaattcc gttgacccat
    22801 tcgtctgtcg ctgctaaaat ttcacggggt ctgtctttcc cagacttcac cagtccaatg
    22861 atgcctgtgt cctagatatt ctgcctcctc gccaacgcaa tttattgagg gaaaacacct
    22921 agtgaatata ctggtattac atgggcctaa tctgaattta cttggaactc gtgagccgac
    22981 catctatggc accatcacct tggacgaaat gaatgtttca ctggaaaatt taggaaagga
    23041 gttaggtgtt tcggtcgaaa gccggcaatc caatgtggaa ggggaattga ttacctggat
    23101 ccaggaggcc aaggcacgat ttgagggggt ggtgtttaat cctgctgcct atacccacac
    23161 cagtatagcc ctccgggatg cgattgtgag cgtgggaatt cctatggtcg aagtccattt
    23221 atcgaatatc catcgccggg aaagttttcg tcggcgctct taccttgccc cggtgtcgct
    23281 tggccaaatt tctgggtttg gaccccaaag ttacctgctt ggactgagag cgctggttga
    23341 tgcgcttcag cggacaatcc cattacacca ctaacatttc cgttcacgtt tattgtatga
    23401 ggagtttgat gctgtgataa cgactgcaga atttcgaaat gggtcgcgct tggagctgga
    23461 tgggcaaccc tattatattg tggaatttca acatgtaaaa cccggaaaag gcggtgcctt
    23521 tgttcgaaca aaactgaaag gctataaaac ggggaatgtc attgatcgta catttcgttc
    23581 tggagaaaaa ttcgacgagc ccaaccttga ggaatgcgac atgcaatttc tctataacgc
    23641 tggcgaagaa tatgccttca tggatattag tagttttgag cagtttacct atcttaaaaa
    23701 ccaattgggc gaaaacgtcg atttgctcaa ggaaaacacc gtcgtcaaaa ttttgttgta
    23761 tcatgatgag ccgatcgcgg tagagttgcc tgtctttatg gagttgaagg tcgtggaaac
    23821 cgatcccggt atccgaggag atacggcatc cgggggaacc aagttggcgg ttgtcgagac
    23881 cggggcctcg gtaaaagtgc ctctctatct ggtatccgga gaagttatta aaattgatac
    23941 gaggacgcgc acctatgtag aacgggctcg gtaattcggg attcctgtcc tccagatata
    24001 cacgaaatag gagattatgg ctgattcatc cagaagagaa atcgaagacc ttgtcgaagt
    24061 gttaaaccgg caccacctga ccgagttgga attcgaaaaa aaaggtgtgc gaattcgcat
    24121 tagacgggat catgtgccgg ctgttacttc ccaccagata gacggatcaa agccagtgga
    24181 cactggctcc tcattggaga agccggttcc catcctctct accgagcccg cacatttctt
    24241 gactgtgacc tcgcccattg tggggacctt ttatcgttcc ccttctcctg atactgatcc
    24301 ctatgtcgaa gaaggtgata ttgtcaaaaa agggcaggtg ctttgcattg tcgaagccat
    24361 gaaactgatg aatgaaattg aggcggaaac cgatggcaaa gtcgtcaaaa ttctggtgga
    24421 aagtacgact ccggttgagt tcggccagcc gctctattta attgatcctt tatcgggatc
    24481 ttgagagctg ctgggttgtc ctgatagtga tttggagagt cacggttgct cataatgatt
    24541 agaatggctt tctgaagtct ttttatctta aggatacgac gtgtttaaaa aaattcttat
    24601 tgccaatcgt ggagaaattg ctctgcgggt gaatcgagcg tgccgggagt tgggtattcg
    24661 caccgtcgcg attcattcag atgttgatgc tcaatcactc catgtccgtt acgcagacga
    24721 acatgtctgt gtcggacctg ccgaaggggg tctgagttat agaaatattc cgaatgtctt
    24781 gagtgctgcc gaaattaccg gagtggatgc gattcacccc gggtacgggt ttttagctga
    24841 aaatgcgcat tttgccgagg tatgcgagtc gattgggatt acctttattg gaccaacttc
    24901 cgagcatatt gctctactca gcgataaatc aaaagccagg gcccacatga agaaacgtgg
    24961 aatccctgtg ttgccaggga gcgatggtga attaaacgat cccaatacct gtgtcgctat
    25021 tgccaaaaag cttgggtttc ccgttattgt gaaagcctcg gccggtggcg gggggcgagg
    25081 gatgcgggtg gtctggcatg aagaagagtt ggcccaggcg atcgaatccg ctcaattaga
    25141 agcccaaaca gcctttgggc atggcggtgt gtatcttgag cgattttttg aagatccacg
    25201 gcacattgaa tttcagatta tggcggataa ccatgggcat gttgtgcatt ttcatgaacg
    25261 agattgttcc atacagcgtc gctatcaaaa ggtgttggag gaatcccctt ctccggcgct
    25321 ggacgattcg ttacgacgca aaatggggca ggtggcagtt gaggccatca agtccgtgaa
    25381 gtatcgaaat gccggcaccg tggagttcct catggatcaa agtggaaaat tttatttcat
    25441 ggaagtcaac acgcgtattc aagttgaaca tccggtcacc gaaatggtga caggagtcga
    25501 cctgattaag gagcaaattc gtattgctgc tggggaagag ctgtcctatc gacaaaaaga
    25561 tattcaattg accgggcatg ccatcgaatg ccgaatcaat gccgaatgtc cggatcgatt
    25621 tacccctagc cccggtacca tcacaaaatt ttgtccacct ggcggaccag ggatacgcgt
    25681 ggataccgcc atggatacca ccggtattgt ccatccgtat tatgactcga tgattgcgaa
    25741 gcttattgcc tttgggcgag atcgagagga agccatggct cgtactagac gggcgttgga
    25801 tgaatttgtg attgaaggaa ttaaaaccag tattcctctc catcggcgga ttcttgatca
    25861 tcctgatttc caaaagggtc ccgtgtccac tcgatttttg gaccggcttg ttgctttaca
    25921 gtccccatga gcgcagtccc tatttctcgg atttccctcc ccactgtttt ttctcggttt
    25981 tatctcattg tcgatgacag ctgggcctcg cgatgttcac tggcggaggt cgtgcagcag
    26041 gctggcgaag caggggttaa gctggtgcaa tatcggaata aaaccggatc gatgaaacag
    26101 gcatatgagg ctggccgggt tctgcgaaat gtggcggcag agagggacat gacgtttatt
    26161 gtgaatgatc gatgtgattt ggcgttggct ctcgaggccg atggtgttca tcttggtcag
    26221 gccgaccttc ccctacttct tgcacggaaa gttgtcggac agaaaatgtt gattggcgtt
    26281 tcgacccaca atcctgaaca ggtgcgatta gccacggaag aaggtgcgga ttatttaggg
    26341 tttggcccta tcttttcgac aagaaccaaa tcgaatcatg agccggttgt tggtattgaa
    26401 ggactggctg gtattcgaga cctgaccccc cttcccatca ttgccattgg tgggattgtt
    26461 ccggattctg ttccggcact ccaagcggca ggtgccaatg gagtagcggt cgcttcggcc
    26521 atactcaatg cgagtgatcg ccccaaagcg ttggcgcggt ttatggctat ttttcaatga
    26581 ggtggttggc caatcgttga atttcaggat ggttggctaa gttttccatt ccctttcgcc
    26641 agtccgtctg cacggttttt tgatattcca aggggccaaa taccggtacg gtcgaccatt
    26701 catgaatcag accaatcgtg gaggttcgct gccgtatgaa acactcgttt tgggttgagg
    26761 gcaggggatc attcaggacg attccgcacg gcttaatgcc tgcaccgtgc agtgcttcca
    26821 aagtcaggag caaatgattc acgctgccaa ggcttgttct gctgaccacg atagcaggaa
    26881 gattcagcga cacgatcagg tctcgtatgg tgtgtgttgg tgagagtgga gtcaagaccc
    26941 ctcctgcacc ttccacgaaa cacacggaat gctgttgcct gacgacatga agggcggtgg
    27001 caattcccgc cacatcaatg gctattccat gtgcgcgagc gcaagctaac ggcgcaatgg
    27061 gctgaggaaa ggcatagaga cagattgacg caaagggagg aggaggggag agtagactct
    27121 gaagacggcc tgtgtcggaa gtctctggct gttgaggatc gagaccggtt tccaccggct
    27181 tcatgacgcc cactcgatgc cctttttttt gcaacgccag aaccagagcc gccgtgatgg
    27241 ttgtttttcc gacaccggtg tcggttgcgg tcatgaatat ggcgagatgg tcagattgag
    27301 cactcatggt cagagatatg ctggaaaatg tgcgggtcac caaatccggc tgtccagatt
    27361 ccatatttgt ccggatgtgt taggcgataa ggccaggtga tagacagttg tagccacata
    27421 ttgtaatgaa gaaggtctcg ggagagtatg gttgcctggc tccattgccg actgccatcc
    27481 tggttctgat aatggtgagg tgtgccatcc tggaaacacc atatttattc gaatattgaa
    27541 ggctccccat tcctttgcca ctgtctgcat caatccagtc agtcctgctt tggaagccgc
    27601 ataggcggct tggcctgcca ctccttgttc agcggaaaga gagcctacaa aaatcacggc
    27661 cccatcatgt tgttgttcaa aaatgggggc gatagcttgg agcacagtat aggcaccggt
    27721 caagttcgtg tgaagggtac gggtccagtc ctcaggggag gttttgagta agagttttga
    27781 aggggctacc ccgacggccc acaccagtac gttcagagaa tggtgggact ggatgaactc
    27841 ctgcagaatg gcctgaattt ttaagttgtt ggcgacatcg gcttgataca gatgactttc
    27901 tcccccggcc ttcctgatcg ccgccgcagt tgcattcgct gcggtttgat gttgatggta
    27961 atgaaggcct actctccagc cctggtgtcc gaaaaccttc gcaatggctc caccgatgac
    28021 tccggatcct ccaaatacca tgatgttcga acgggtgttc atcttaccga ctattgattg
    28081 gcgatggggt tcattcgtaa atggggagta cagcgtgtca ctctcatgca tcgttgaaaa
    28141 agtgaaaata atgagattag gggttggtat aggactggac tggcttggtg ccgttttgta
    28201 ttgattacca ttcggtttcc ttgtttctta gtctgcatag gacaaagaga ttgtcaatgc
    28261 ttgaatggtc tgcaaacaaa aaatgcggaa tttttagggt gaatgggacc tggatcgttg
    28321 gtccttgctt ggatcttgcc cgttttaatg atcctatggt aggttcacat cactttctgt
    28381 ctatgaatat ttcctttaca ttgcttggaa cttcctgcag gactggagca ttggtttgcc
    28441 ttggtctttc cttgttgtgc ctacccggca tggtctattc ttccgattcc acgaagttta
    28501 ttgttgacaa aagtcatgaa tattttcctg atcaggtggg aaatgaatgg gtgtataagg
    28561 gaacgatgat cgaaggaggc atggtccaga ttaagaatga ggatacacag tttacgaata
    28621 tctcaaaagt gaccgggaca gaaatcctgg agggagtgga ggttacggtt ttcaacgata
    28681 ccaatcctgg gaatcagggc ccgtcggata gttattatcg ccgtgatatt gcagggatcc
    28741 gatattatgg gtcgaaaccc gggacgacgt tggagcgcca attggtgccc tatcaaatcg
    28801 tgcggtttcc cctagagatc cctacctctt tcgagcaatt taaccggacc ggggtggatt
    28861 ttggaacaga tgtggatcat gatggagaaa aagaaacggt ggatattgag gcaactgtct
    28921 ccgttcaagg acaagagacc gtgatcgtac cggctggcgt atatccggaa gccattcgcc
    28981 ttgaagccaa aatgattatg aaggtgcatt tgtctgagag tggagagaca atccaaggaa
    29041 cagatacgat gacggcctgg tttgctcgtg gggtgggatt gctcaaatac attgaacggc
    29101 aaaccattcc ttccgttcgc agtcaaaatg aacggatggt cgaagtgatt gaggaattgg
    29161 aaaaggtgac agtggcatct gaagtggcat tattgcgtcg gagcgaagcc acggcgaagc
    29221 gtgttttccg taatcacttt ttccaccatg aattgctcaa gatacccgct tccgccggct
    29281 ttccggccga ttcctgataa tcgatgccct ccaaaaggct gccggttcac caatgatccg
    29341 gtaatcggtc gattgatata taaattcccc acatcaaatg tgtcgcgagc cagttggata
    29401 ttggcaggac tgcgtgaata gattcctccg gtaagggcat agggggaatt attggctaat
    29461 tccagagctt cagaaattgt agagaccttc aacacaacca tgaccgggcc aaatatttcc
    29521 tcctgaacga caggatgcga agggggtaga tctgtgagta tcaccggccc ttgaaaatac
    29581 ccaggtccct caatctgaca atcgagaagg cacgtaccat ctgttttccc aagttgcaca
    29641 aagtgccgga ctcgttccag tgcccgttga tcaatgagtg gtcccatccg gtttccaggg
    29701 tgttcgggtg gaccgatcgg aatgctagag gcggcctgtt tgagtcgttc aataaataag
    29761 ggatacacat tgtttaggac aaggattctg gagcaggcgg agcatttttg tccttggtag
    29821 ccggttgcgc tggctaatgc cccaattacc gcttcatcca aatcggccgt ttcatcaaca
    29881 atgatcgcat tttttccccc catttctgca atgacatgtt taatatgtcg ctgtccagcc
    29941 gacacctgac cggcctcccg gataatattc aatccaacgg tttgtgatcc tgtaaaggca
    30001 atgagatgga ccttggggtg tttgacaagg gcttgtccca cgtccggccc accaggaaga
    30061 aattgcaaga cgccttcagg taacccagct tccatgagca gcgtgaagag gtgatacccc
    30121 atcatcggtg agcgctcgga tggtttaaac aggacaacat tcccgctgac taaggctgcc
    30181 gaaacaagac cggtggggat ggctaacgaa aaattccacg gcgcaatcac cacggccaat
    30241 cctcgtggtt ggaagatccg ttggttgtat tcccctggct ctattcccag gcgctgcgga
    30301 gtgcctaacc gaatcatttc ccttccataa aattccagaa aatcgatggc ttcagcgaca
    30361 tcggcatcgg cttcccgcca gggcttaccg gtttccagta tttcccacgc ggctaattcc
    30421 ggtctacgtt gtcgcatgag tccggctgca tgaaataaaa tccgcgcacg ggctttcgga
    30481 gggacctttg cccaggtacc aatatggtcg tgggcgatgt gaacaatcgg atcaatctgg
    30541 tcaggagacg gagttggaaa tttggcaatg atttgcccag gaaaactggg attagtcgag
    30601 atcagttctg ctccaaacca ttgtatgggg gtcggaaccg gataagaatg gatttgtccc
    30661 aggaggggac cgatgtgatt gagagctaga gaaaaatctc gttgaacgtc ctgttgagaa
    30721 aaatccgaat gtggttcgtt ggagaacctg tcgggactgt ctccagcgag tacagtggct
    30781 tccgactgaa tttcccgaga catcgtctcc tcttcattcc tgtgagcgtt cgagggtgcc
    30841 aatagcagat gctccaggga ttcggccgtt tcatattggc gtcgaataaa actttcattc
    30901 gaggtatttt ccaataaccg acgcacgaga taggccatgc cgggaagaag ttccccaatc
    30961 ggcgcataga ctctaagccg aaatccctgt tgcaccacgg catcacgtag aggttcggcc
    31021 atgccatata acatctgata ttcaaacgtt cccggcggga gattgagtga gtcgcccatc
    31081 gcctggacta cggctaaggt tcgaatattg tgcgagccaa aggcagggcg aatgaaggct
    31141 cgatgttcca gaattcgctg ggcgaggtgt tcgtagttcg catccgtgtc ggtttttttt
    31201 aagaagaccg ggatgggcca accttgttgt tgatagcgaa tcacttcaga gtcccagtag
    31261 gcgcctttga cgaggcgaat accgaaggga gtgttgcgct gttttcccca tgctagaaga
    31321 gtatccagtg tgtggttggt gtgcttgagg taggcttgaa tggcgatgct ggcatgcgga
    31381 aaatgttggt aggcaggttc agaaaataag cgcataaata ccaaaagaat gagcggctcc
    31441 aattcggcct gttccatatc aaatgtgagg gaggcaggga gggcctgcgc aagctccagg
    31501 atcgatcgta gtcgaggggc aaccccagcg taactgctct caggatcaat cgggtctaat
    31561 tgcgaatata aggcagagag tttgatggac agattgatcc tgggaagaga cccaagatga
    31621 tcggtttcta atagaggttg atgattccat tgtgggataa tctcatgtaa ttgggtcaat
    31681 gtctgaaggc atcgcgtgtg atattggtcg gcctcctctt cactgaccgt ggattctccg
    31741 agcaaatcca gcgaacacgc acttcctgct tcccaaagtt gagtgagcgt cgggacagcg
    31801 tgaccgacgg tttcccctgc cataaatgtg taggccattt tcaaaaattg gcgtcgcaat
    31861 atccgtgtcc caacttgggc cgtaagggaa ttttttgaaa accggtgcag gagccactgc
    31921 aggggaccag ggagagtcgg cagatcttgg aagtattcct tcaggatacg cacaaattgt
    31981 gcgtctgtct gaagggaggg aaggacgtca ataaagcgaa acaattgcac tttgaactgt
    32041 tcatcacggg ttccccaatc tagaagtagt gaggtccacc accggtgatc ccaaaatccc
    32101 ggtgtatggt gatgagcgta tttggctaat gtttgtccaa tgcgccgaat gtcaggttcc
    32161 aacgaatcat gggcaatcat ccgcattttc ctcctaaagg tattttacac cttttcggag
    32221 aatggggaag agagatgagg gagaggaaaa ctcagaagaa ggattagggc tcgcaagcag
    32281 agagacgttt gccggaaata ctgccatgag gaccgaggga atttttgaaa gtgccttcca
    32341 aatgagtgtc gttgttgctg agcagcaaac tatcttcctg aactccttgg ggaaaagtcc
    32401 attggagata gaccgtatcc ccgtgaatct caatagtggt cggtgcgatt tgagacggcg
    32461 aggattggtc aggtggtgaa aaggtgacag tcattttacg atcgccatgg ttttggtggt
    32521 tatgtactat ccatcgccag gtaccgcaca gccgggtggt gtcaacattg gcttgttcca
    32581 gtttttgcca ctgctgaatc ttttgccagg cttcccatgc tgtcctatgg gcggtggcct
    32641 ccaactcgct gatttttgtg agaagaatca ttctaaacat agggaaaagc cgggtttcaa
    32701 tataatgctg agtataggca tcatgaattg gttgggcgga ttggtgttga tcggctatgt
    32761 gatcgggagt ttgccaatac tcgagtaacc tctcctcgat tcctttttgg ccgtgctgcg
    32821 tgaaagtact cgtccaggat tggggactat tatcccaatc cggatacgtt tgatcgtaat
    32881 acgaggcaaa tttggcaaat tcttcgctag ggggaggtgg gtgagaagtc atcgtgtgag
    32941 accaagccga tacttgtttg tagccattaa tgacagattc caaggcttga gggggcgagt
    33001 gtttcgtgat ccatttcagg tgtgcatcgt cggggatgat gtcggaaagt ggtgggatac
    33061 ctattgttga tttaataaga atctcccgaa atgtttgcgt atgaatggta gtagccagtg
    33121 agttcaagta gatgatgatc gcttccttga tctctgctgg gagattcaac tgcgtaggta
    33181 acagttgtga tttcatgtga gagcgtgaac ctctttcacc tggaatggtt gtggctttga
    33241 agagggctaa agcctcgtca tcagaggaaa gctgaagaac ttgtgtacga tgtttttcaa
    33301 ggagggacca ggggagccta gttgattcct gatcggcaaa ggcagtcgaa aggaaacaga
    33361 aaatcaagac tttggcgcaa acaaaccagg gaatcaacca aacatttcga ttgccatgcc
    33421 attccatttt tcaaaaaaaa agttggcggt gtatcgggat aaatggatga aaggagtcag
    33481 gcatggattt caaaaatcaa acgatgctta tggggttttg tgaatgatcg cgttaatgaa
    33541 gtctattgag gtttgtagca cgtcttctag atctgatgcc cggtagctag gagtgctact
    33601 ttgccagaat tcatcggagg cgtcgggata agatcgcact tcgatctttt tcccggcctg
    33661 gttggccatt tggcaaaact ggtcacgttc ttctgctgaa ttgagtaggc ttttccccgg
    33721 tgtctggagc agaagggggc agtataaacg ttgagccaat tcagggtcgg ttggcagagt
    33781 cccagagatt gccactgcgg cctgaaggcg acgtcgatgc gcagcagttt tgattgccag
    33841 ggatccgccc attccaaacc cgatcacggc atgcgcattg cgggtggttc tttctaaatt
    33901 tatatcctcg gtcaggttag cgttgagaaa ttcaaagctg gcattgatgt cttgtaaggc
    33961 ttgttgctca ttgactcgct ccattaaggc atcggcaact tcagcattgg ccgtcaccat
    34021 cccgccctgt ctcacataca aattgggaac taagaccacg tacccttggc aggagaggcc
    34081 ttttgctaat tcctgaatat gggacgtgag tccatagcga tcatgcagga ggaccatagc
    34141 cggtagtatt ttgttggcct cttggggggc gaattgaaga ccctccacct gcgggccttt
    34201 agcatgtcgt gtttccgcgt agggatccac catgtggtct ttgtgtgttt caaaaattcc
    34261 tgaactatta aatcgcacaa cggtatatcc aatttgttca atgtcatagg gggtggtgtg
    34321 ttgggtcatc agaatagcct ttggataaga atgagccgag ctagcggaaa agaaaaaatc
    34381 attgaaaatt caatggcaaa agtcaaaaca ctatatcgat ggattctttg gcctgtcaat
    34441 cgagcatctg ttcttcagag cgggaagcgt taaacgaaga ttttttattg gtttcgtctt
    34501 tcagaggaac gattccattg gaatgcattc tctggtgata agagttcggt catgccatat
    34561 ttcctcacat gggaagccat ctcagaataa ttggcatcac tttttcgagg aaaaccacac
    34621 cacattcacg caggaaaaaa tcaactcaaa tgctattgtg cgtttcgtta attttgtaat
    34681 cagatatttc ttgctaattc ctaaaacgat cagtttgacc accttccaag tccatttttt
    34741 cttgatacaa actgtacttc ctgaatccta gaaaagttca ttagggaata ttgccaatgt
    34801 ggtggctttt agtgtaagat ggcgcctgca gtggaatgtc agaaggttgg atatcgggat
    34861 aaggaaagga gttgagcatg gaacggcgag tcacttccca tacggaagaa gaaga
//
LOCUS       DAORAQ010000018        33929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_270_length_33929_cov_62.686574, whole genome shotgun sequence.
ACCESSION   DAORAQ010000018 DAORAQ010000000
VERSION     DAORAQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 33929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33929
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_33929_cov_62.686574"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKM72_03235"
     CDS             complement(<1..228)
                     /locus_tag="PKM72_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59826.1"
                     /translation="MGLAGGGFLRLGLGLSGPLLVTRYLDLYFPNFLNESLHPLEGSV
                     IHKQREQNRLLVTITTQDGTVLATFQQQVQEI"
     gene            complement(233..556)
                     /locus_tag="PKM72_03240"
     CDS             complement(233..556)
                     /locus_tag="PKM72_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59827.1"
                     /translation="MEVITLLIAVVALVIAILAFQRTGGGIRDLRRQMEDLSEKSEHA
                     TKGARDMTADALSRLETFIRGQQKPTPTEQESREGEPPITEPLTSPTDRQSRKEEPPS
                     TEQKP"
     gene            667..846
                     /locus_tag="PKM72_03245"
     CDS             667..846
                     /locus_tag="PKM72_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59828.1"
                     /translation="MNDLVSQNLTVSLMSVSGNFGETWNNPFWIYIYGFGSNRHLSIA
                     YTIELGRLHHIHLAK"
     gene            1010..1231
                     /locus_tag="PKM72_03250"
     CDS             1010..1231
                     /locus_tag="PKM72_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59829.1"
                     /translation="MKELRCEPVLNTMVDPDTLERSVGESPFCNKILGLTNDELDFYF
                     GGLSNFESPLIHAQDGVATANVRIDQLLL"
     gene            1302..1444
                     /locus_tag="PKM72_03255"
                     /pseudo
     CDS             1302..1444
                     /locus_tag="PKM72_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558324.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1468..1659
                     /locus_tag="PKM72_03260"
     CDS             1468..1659
                     /locus_tag="PKM72_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59830.1"
                     /translation="MPRIVVSLPLPIETFSLRAMHYQSTQFSERNNYSVLCGVLVNGF
                     CEQWTHEKTLNDGMTVTSV"
     gene            1768..2244
                     /locus_tag="PKM72_03265"
     CDS             1768..2244
                     /locus_tag="PKM72_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59831.1"
                     /translation="MAKRLRVLLVVLTVIFTGMGTESGLAEEIRPLPSILSDVERHIG
                     ELTINLDDIRKRIDFLQNLPVTHDPVIQELRALDLQGWTLHREQWELQLEHLRFAETM
                     LRKVQGISGEPHELRAAWRLHEGQYRTSLEGYRRQRDTIEERRLITEGRMFERYLP"
     gene            2241..2753
                     /locus_tag="PKM72_03270"
     CDS             2241..2753
                     /locus_tag="PKM72_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59832.1"
                     /translation="MSERHATKHMPVWRDMVFIGLLGSGIFMTGCTSVNTLLLTSQTF
                     PPKASWQEVEILIREPSCAHIDIAQLSMEGSGSGSYATEQTDILKKAASLGSDAVVFT
                     KSEKYLEHGVSYQSPMMMGGPWGMGSYGYPGWGYGMGYGRGMAIPYNYTVTSLTGIAI
                     RYTNNAQTKC"
     gene            3034..4440
                     /gene="sthA"
                     /locus_tag="PKM72_03275"
     CDS             3034..4440
                     /gene="sthA"
                     /locus_tag="PKM72_03275"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNP59833.1"
                     /translation="MSHQFDFDLLCIGSGPAGQRAAIQAAKFRKRVGVVERRQCAGGV
                     CIELGTIPSKTLREAVLSFMATNHPFVQQTRNRERHRPTAEQLFSRVQFVRNREVDVV
                     EDQLWRNDIELISGEAAFTDAHTLIIHTEQGTKTVTAENILLAVGTVAAPPPGTGVDG
                     EIIVTSDEMAHLTSLPKRLVVVGGGVIGMEYASMFAALEIDVTVVDKREHLLEFLDNE
                     IVEELIHQMRNRDVTFRLGETVGALTIAEGPPKQAVIQLESGKRLVSDMVLYSAGRIG
                     ATDRLNLAGAGLTADARGRLAVDQDFRTQVPHIFAAGDVIGFPSLASTSAAQGRRAAC
                     HAFGVSAGAMDTHFPFGIYAIPEISMIGAHEHELTEKKIPYETGVARYKEIARGQIMG
                     DDNGLLKMLFHRETRKLLGVHVIGTGATELIHIGQAVLELGGGLDYFLDRVFNYPTLA
                     ECYKVAALNAFNKLSQIG"
     gene            4902..8081
                     /locus_tag="PKM72_03280"
     CDS             4902..8081
                     /locus_tag="PKM72_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP59834.1"
                     /translation="MNQLVLIALRRPLTFVVLAILIVIFGTKSVLQTPTDVFPNIRIP
                     VISVVWSYAGLLPSDVSGRITFYFERALTSTVEGIKKIDSKSYFGISIINIFLQEGVN
                     LAGAEAEVAAIAQTVVKALPPDISPPMIMRLEASSVPVAMLQVTSDTLTPAELYNLAF
                     TQIRPLLVTIPGAILPHPYGGQPKQLLVSLDQQKLLARNLTPADIFHAFDRQNKVLPA
                     GDQKIKATDWMVQTNAMPLQVEDFNDIPIKREGNAFIYMRDVANVTLAGPPQTNAVLV
                     EGKQAVTLVVMKSGEASTLEVVDGVKKAIPRIEKIVPKGVEVKILNDASLFVKESIAD
                     VVKEMATASALVALIVLMLLGSWRPTVIIATSIPLSILSALICLNLAGESLNLMTLGG
                     LALAVGILVDDATVMIENIDTHIAMGKPLEEAIVDAANQIIVPTLVATLSIAIVWLPL
                     FELSGVSGWLFAPMAKAVIFAMLSSFILSRTLVPTMAKYILADHHAPQANEAGGGGEP
                     AKPQSFFVRFQQGFERGFDRFRERYNARLEQVILHRRTFVAISLALALASLSLFFING
                     RDFFPEVKSGTMQMHMRAPLGTRIEVSSRLSSLVSKDIAQMLPGQVEGVVSNCGLPVG
                     PHNLAFIPTPTIGSQDCDLTIDLKNEKSPVWDYRRILRKGLSERYPGTEFTFQPADLT
                     AKILNFGSPAPIDVQINGMDMYANYEFARKLAGELRKIPGARDVTIQQTMRTPTLFVE
                     GQRTFGLGTNLSEGEIADNLLLSTSGSQQIDQQYWLNHKTGISYQINIYTPQPQLTSI
                     KNLLTVPVDQNNLDSSQDDVQLLGNVTTHSMIGTPGLISHANIMPLFNIYVSAEGRDL
                     GGVLADVEKAAKSLEDELPRSAALEIHGQAETMYEAYVELIVGLIFAVVLVYLLIVVN
                     FQSWLDPFIIITALPGALAGIAWALFLTHTNISVPALTGAIMTMGTATANSILIVSYA
                     RERLEMHGDAMLAAVEAGKARIRPVLMTASAMIIGMLPMSMGNSPNAPLGRAVIGGLM
                     VATVFTLFFVPCVYAIIYNRRGARQKDHG"
     gene            8083..9324
                     /locus_tag="PKM72_03285"
     CDS             8083..9324
                     /locus_tag="PKM72_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP59835.1"
                     /translation="MKILRGRGLVIFAILLSLLYMGYRIYEHNSGAAMLREETLADAV
                     PIVAIINPKPLPPTQTITLPGNIQGWFEAPIYAQVSGYVKMWYTDYGAKVKKGDILAK
                     INAPALDAQYRQAKADLASERALYGLAKLTAKRYLALREKHSVSEQSISVKVAEANAQ
                     AARVNAAEQNVKNFEALIRFKTIVAPFDGVVIDRNINVGDYVNKEGTISSPTAISNLF
                     TVADVSKVRLFISVPESFGAFLKPGLTAEVTVPQLPSRRFTAKFLTVAGGFNVSTRTA
                     VTEFVLDNEDRALWPGSYAKVSLTTPVHSDFLTIPTSAMVFQENGTEVAMLTDEDRIH
                     FKSIAVGKILDATVEVTEGITSTDRIVNNPSAALLENEKVRIVTPAAGYDRLNTDGPA
                     AKEVSVPMDNPAPTEAAASTE"
     gene            9435..10961
                     /locus_tag="PKM72_03290"
     CDS             9435..10961
                     /locus_tag="PKM72_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNP59836.1"
                     /translation="MLNLSACLPHVDLAPDYQPAEFVVPVSWHGASPFVEAKPSDGEL
                     RRDWWTLYNDPVLNTLEEQAMVANPDLQAAAERFVQARDMMMKARSRRIPQVGLGFGT
                     SRNRQSDNHLFRDPDISDTASTVSIGGIASWEPDFWSAIRNATRIEMYRAEERAAEYG
                     LARLSLQAEIAANYFTLRGFDAQLAIYTKSIDLYKGSLELITNQYTVGIASQLDIARV
                     ESLLFSTQTKEAEVRGQRQVTEQAIAILLNRAPASFTIDPVADLRVENFTIPKTIPST
                     LLERRPDIARMERRMAQANRAIGIARAAFYPNVTFLAGGGFEDSGFDLIKMANSFWSY
                     GSAIELPLFQGGFRRAQLQQSWSVYRETEDRYRSTVLNAFREVENNLSLTNQLTTAAK
                     RQDAAVGAFLKTQDLTSSLYFGGLASSLELIYAEVNTLAARIDAVVIKADLLKSSVAL
                     IRALGGGWNRNQLPADEQIQPFDTFQYVDLDKPPPAGGIDVNADNNSVNNDLTKPSIP
                     "
     gene            11288..12631
                     /locus_tag="PKM72_03295"
     CDS             11288..12631
                     /locus_tag="PKM72_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNP59837.1"
                     /translation="MASALLYDRLQFAFTVTFHYLFPQLTIGLALLLLYLRSRALVSG
                     DEHYHQIARFWTKIFALSFAFGVVTGIPLEFQFGTNWAKFSNFAGGVIGQTLAMEGLF
                     AFFLESSFLGIMLFGEGRFSRRVQWAASLMLFLGSWLSGYFILATNSWMQHPVAYTVA
                     PDGRLFVDSLSSLLTNPWLFWQFAHNMTAAVVTASFVMAAIGSFYLLSGLHVKEAKTF
                     LRTGVVAGAIATALMIFPTGHENARQVFEHQPVKGAAFEGLFKTERGAGMVLIGQPNI
                     ETMTIDNPLVVPDALSIVLFDELYAEVKGLDAFPRSEWPDNVALLYYAYHVMAGLGTI
                     LVSVMGLALLWLWRGRLFTANWLLWLLMLAAPFPYLATTAGWMTAELGRQPWLVYGLL
                     RTADGVSPLVHSGNALFTLLGYLGIYLVLGLLFLFLIADIIRHGPESSIPKSANL"
     gene            12644..13678
                     /gene="cydB"
                     /locus_tag="PKM72_03300"
     CDS             12644..13678
                     /gene="cydB"
                     /locus_tag="PKM72_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNP59838.1"
                     /translation="METFWYVAVTFMLAVYVVLDGFDFGLGIVYSFVAKNETDRRAAL
                     GAIGPIWNGNEVWLIAAGGLLFFAFPKAYSAGFSGFYLALHLVLWLLIARGLALELRS
                     HVNHPLWRQFWDVAFAGASLLLAVVFGVALGNLIRGVPLNPEGYFFVALWTNLMTGPE
                     PGILDWFTILIGATSAAILAMHGANFLAMKTEGELRKRARRAAWLTGSAVVILVVLAL
                     TAVPFVQPLFFLNYAAHPMGYVFPVLTMVTLLLALEIRRRDWDAGAFSATSLLILTML
                     ASTAWGSYPNILIATSEPANSLTVTNATAGVYGMQIGLWWFLVGFILLLVYQACAHQA
                     FWGKVKIDAE"
     gene            complement(14332..15135)
                     /gene="modA"
                     /locus_tag="PKM72_03305"
     CDS             complement(14332..15135)
                     /gene="modA"
                     /locus_tag="PKM72_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNP59839.1"
                     /translation="MTGIRKLGMAITLVVGILGIVATGEPVQAEALTIGAAPSLRSAL
                     REIVPMFEKEYDAAVKVVYGPSQTFRRQIEKGAPIDVFLPAAVGEVEKLQKKKLTLNG
                     GPRVYAQTSLVLVMSASSHVMPISFREALPNGAIRMALGDPKTSSLGEITAQALAKRD
                     PAYKNRFKLVHAAHAEDIVDLIHTGKADMGIIYRVDAINNASVRIIDENPAGMHIPVL
                     FGEAVVSTCREASLNVAEEFFDFIMSPRIQKLLVKYGFDHGFDSVSSNG"
     gene            15716..>15972
                     /locus_tag="PKM72_03310"
     CDS             15716..>15972
                     /locus_tag="PKM72_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP59840.1"
                     /translation="MNQIVLIALRRPYTFVVMTILIVLLGVNTVRHMPTDIFPNITIP
                     VTSVVWNYSGMLPQQVEGRITYLFERFLTSTVEGIKYIHSHS"
     assembly_gap    15973..16072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16073..17032
                     /locus_tag="PKM72_03315"
     CDS             <16073..17032
                     /locus_tag="PKM72_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP59841.1"
                     /translation="GQDMQVMVNLDQQKLLARQLTPADVHNALQQQYLVLPSGDVKIK
                     ETDWIVMTNASPLKIEDFADFPIKREGNAFVYLRDVATVRLMGRVQQNSVLVKGKQTV
                     ILVAMKSTEASTLDVVDGIKKMIPRAEQISPEGVEIKLVNDASTFVKDSISDVLHEML
                     MAGGLVGLIVLLLLGSWRATVIVWTSIPLSILTAIIGLHLLEETINVMTLGGLALAVG
                     ILVDDATVMIENIDRHIEMGKLLEQAIIDAANEIVVPTFVATLCIAIVWLPLFELGGV
                     AGYLFKPMAEAVIIAMLASFIFSRTLVPTMAKYMLKDHHVAAA"
     gene            17016..18728
                     /locus_tag="PKM72_03320"
     CDS             17016..18728
                     /locus_tag="PKM72_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP59842.1"
                     /translation="MSQPHEPHGELNAEGSRNIFVRFQNGFERAFNRFRGGYGALLEQ
                     AMANRGVFVATALAIAVSSLSLFFFLGRDYFPEIRSDIMTMHMRAPLGTRIEVSGRIA
                     TLVSNSIKELLPGHVENIVSNCGLPVGPHNLAFIPTPTIGSQDCDLTILLNDEKSPVW
                     EYRKILRKGLRDRYPGTEFTFQPSDLTAKILNFGAPAPIDVQINGPDMYPNFEYARKL
                     VGKFREIPGAADVVIQQTMATPTIMVEGNRTFGLGVNRSLKDMADNVLMTTAGSQQTD
                     QIYWLDPSTGMSYLINVYTPQPQINSVNSLKTIPVQASSTSSKDPEGQEMQLLGNLAD
                     ITATGTPGVITHGNIMPLFNIYVSAEGRDLGGVLADVEKIAKDMEDEMPPTAALEIHG
                     QASLMHDAYFELIVGLFAAVVLIYLLIVVNFQSWLDPFIIITALPGALAGIAWALFLT
                     HTRLSEPALTGAIMTMGTATANSILVVTYARDQLKKHGDALRAAVEAGTTRFRPVLMT
                     ASAMIFGMVPMATGYSANAPLGRAVIGGLLMATLFTLFFVPCVYAIVYNRRAARQKDH
                     IQNE"
     gene            18748..>19915
                     /locus_tag="PKM72_03325"
     CDS             18748..>19915
                     /locus_tag="PKM72_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP59843.1"
                     /translation="MVISAALLCVLYLGYRVYESKSDASLLREETHENAVSTVAVIYA
                     KPVPPTETITLPGNIQAWFQAPIYAQVSGYVKMWYKDYGALVKKGDILAEINAPALDA
                     QYAQAKADLESVRAIYALAEITAKRWLALRKNHAVSEQSISVKVAEAKAELAKVRAAE
                     QNVRNFEAQIRFKTIVAPYDGVVTVRNINVGDYVNKEGTISSPGSVSNLFTVADVSML
                     RLFVSVPESFGPFLQPGLTADVTVPQLPNRHFTGKFLTVARGFDESTRTAITIFTIEN
                     EDRALWPGSYAQVHLTAPVDREVFMIPSTALVFQEHGTQLAVVGEDDQVHFNPIVVSK
                     LLDSAVEVAEGVSTTDRIVNNPSAALLEGDRVRIVTPAPGYDQLNAPAPTKAAAP"
     assembly_gap    19916..20015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20098..21642
                     /locus_tag="PKM72_03330"
     CDS             20098..21642
                     /locus_tag="PKM72_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNP59844.1"
                     /translation="MLLLTLNLPACSWFPAVDLAPSYEPPQYVVPASWKGASPFVEAN
                     PSDEELRPDWWQLFNDPILNSLEEQAMAANPDLQAAAERFVQARDVMMRARSQYLPHA
                     GLGFGASNNRQSDNSLFRGLGEDNREVSITTGGLASWEPDFWSALRNATRAEIYRAEE
                     RAAEYGLARLSLQAEIGANYFSLRGFDAQAAIYTQSIDLYRNVLNLVKAQFAGAIASA
                     LDVARVESLLFSTETKLAQIQGQRQVTEQAIAILVNMAPSSFTIEPVDDLRVADFEIP
                     KTIPSTLLERRPDIAGMERRMAQANRVIGIARAAFFPDVRFSAGGGFEDTGFSLIKLA
                     SSFWSYGSTVSLPLFQGGYRRAQLQQAWSAYRETEDRYRSTVLNAFREVENNLSLTNR
                     LTLAANRQGAAVGATLKTQNLTTELYRGGLASSLELIYAQVATLEARIDLVAIKAELL
                     RASVALVRALGGGWNRNQLPTDEEIQPFGTFQYVDLDKPSPAGGIDVNAGNNGVNNDL
                     TVPFVP"
     gene            22194..23711
                     /locus_tag="PKM72_03335"
     CDS             22194..23711
                     /locus_tag="PKM72_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNP59845.1"
                     /translation="MVTKNRFLSLTRMNANKAGLAGIGFLAGILIMFGSWNMPFYSHA
                     ANSQPVASASIGSPPNMEKGGFADIAQAVTPAVVNITVRKEAPVPMSGVPSDRMREFF
                     GIPGMPEMPGMPKRRGGPPAPEAQGAGSGVIISSDGYVLTNDHVVDGANEITVTLPDK
                     REFTGKVVGLDPQTDLAVVKVDATDLPFVPWGNSSSLRVGEYVLAVGNPFGLNSTVTM
                     GIISALGRGGMGITQYEDFIQTDAAINPGNSGGALVNTRGELVGINTAIFSQSGGYQG
                     VGFAVPTNLAKPVYESLISTGTVVRGFLGVGIQEMNADLAQSFQLDGTKGALVTNVVP
                     GSPADEAGLQRGDVVVEFQGKPVLDPRSLQHQVIRTTVGSEVSMVVMRNGHKKSVKTV
                     IREQDNPTQVAQASPVEKEGPLAGVAVQDLDSGIAQQLGLDKNVNGVVVMDVAQGSYA
                     ARAGLAQGDVISEINRKPVRSEEDFVKAVSYLNENSSALIFIHRGKGALYLNIKV"
     gene            complement(24119..25525)
                     /gene="zwf"
                     /locus_tag="PKM72_03340"
     CDS             complement(24119..25525)
                     /gene="zwf"
                     /locus_tag="PKM72_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNP59846.1"
                     /translation="MGRTNSRPKQSDALVLFGVTGDLAHKMIIPALYAMTKRGLLNVP
                     VVGIAAPKWSLAQLRKHVTDSIKQAGKIDDPEALDHLLSLLQYVGGDYNDPVTFKALK
                     KTLGNAKRPAHYLAIPPSLFTAVIQGLGGSDLAKDARLIVEKPFGRDLASAQKLNRMA
                     QSVFPEDSIFRIDHYLGKEAIMNILYFRFANSFLEPIWNRDCIDSVQITLCEDFGVKA
                     RGAFYESTGCLRDVIQNHLFQIVSLLAMEPPAYQGYRAVHSEKAKVFQAMRPLRPDDV
                     VRGQYTGYRKEPGVAKDSDVETFCALRLFIDSWRWEGVPWYLRAGKCLATTAAEVLVE
                     LKPPPQRLFDDSRPAAGRGNYLRFRLSPSSAVALAARVKRPGKEFIGDQQELYLLEEQ
                     SGEEKPYERLIGNAMAGDGALFTREDAVEAAWKVVDPVLGKHHPVRPYKRGTWGPKEA
                     DALIATDGGWHNPLSEET"
     gene            complement(25527..26546)
                     /gene="gnd"
                     /locus_tag="PKM72_03345"
     CDS             complement(25527..26546)
                     /gene="gnd"
                     /locus_tag="PKM72_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNP59847.1"
                     /translation="MQLGMIGLGRMGANLVRRLTKAGHTCVVYDRNSAEVKKLEGRDV
                     QGAASLDEFIAKLSKPRAAWVMVPAGVAGETVKELASRMETGDIIIDGGNTYYRDDLE
                     RAKALKSRGIHYVDCGTSGGIFGLERGYCLMIGGEEKIVQHLDPIFSSIAPGVKAAPR
                     TPGRTGASGRDEQGYLHCGPTGAGHFVKMVHNGIEYGLMAAYAEGLNILKHADVGLRD
                     QEHDAETTPLRDPDAYQYQIDIGQVAEVWRRGSVVASWLLDLTAEALVEDPDLTSFSG
                     RVSDSGEGRWTIAAAVDEGVPAPVISAALFGRFESRGNADYADRILSAMRKQFGGHDE
                     KKEKS"
     gene            complement(26571..28673)
                     /gene="tkt"
                     /locus_tag="PKM72_03350"
     CDS             complement(26571..28673)
                     /gene="tkt"
                     /locus_tag="PKM72_03350"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNP59848.1"
                     /translation="MNKAPTSVSSSEQLDQLSINTIRFLSVDAVQKAKSGHPGLPLGA
                     APMAYVLWTRFLQHSPSNPDWFNRDRFVLSAGHGSMLLYSLLYLTGYDLPLDQLKQFR
                     QWGSRTPGHPERGMTPGVETTTGPLGQGFGNAAGMAIAEAYLATRYNRPGFEIVRHFT
                     YALVSDGDLMEGVAAETASLAGHLKLGKLIYLYDDNRITLAASTQLTFTEDRAQRFAA
                     YGWHTQSIEDGNDVNAIDRAIQEARQETERPSLILVRTHIGYGSPNKQGTFAAHGSPL
                     GEEEVKLTKENLGWPVDPPFLVPEEVEQHCRQAVDRGRQAETEWNATFKAYETQYPEL
                     AGELRLLIKGELPPGWDTNIPQFPADGKGLATREASGKIMDAVNRTLPALIGGSADLN
                     PSTYTELKDAGNFEPSDMAVGDLQGSAGGGWSYAGRNLQFGVREHAMGAILNGLATHG
                     GIIPFGATFLTFSDYMRPPIRLAALMGIQVVYVFTHDSLALGEDGPTHQSVEQIACLR
                     AIPKLIVIRPADANETAMAWRVAIETRDRPVALILTRQSVPTLDRAQLSAADGLKRGG
                     YILAEAPNGHPDLILIASGSEVALIVEAREKLLEQKLQVRIVSMPSWELFEAQSEEYR
                     NTVLPPVVGARLAVEAGVSQGWYRYVGNNGDVLAVDRFGTSAPGKMVMQEYGFTVANV
                     CRRALALLEPHDEVITHG"
     gene            29316..29537
                     /locus_tag="PKM72_03355"
     CDS             29316..29537
                     /locus_tag="PKM72_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59849.1"
                     /translation="MGTPHSSPGGSDKGKNLYTAAHQQEHAEQIHDRVDPKPEWKSSG
                     VLVHPSLYLSYLAFTGFLRFHFLVKRELD"
     gene            complement(29561..29860)
                     /locus_tag="PKM72_03360"
     CDS             complement(29561..29860)
                     /locus_tag="PKM72_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59850.1"
                     /translation="MTNDEKMATITRWLNPEERVTVQFLDAQDLNTEVTGCPEQLVDL
                     SIETQVSHIKQHLSIPLSKVELSEDFSHYTRDPERPLQPSRLKLVINEKHPPIIY"
     gene            complement(29914..30132)
                     /locus_tag="PKM72_03365"
     CDS             complement(29914..30132)
                     /locus_tag="PKM72_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP59851.1"
                     /translation="MKALDHSQELRKITTQQIMSKEAYLIHDDTTIKDAISIMKEKKL
                     LNLCVEENGVVTKTLTRHDLLRGYLGGI"
     gene            complement(30178..30315)
                     /locus_tag="PKM72_03370"
     CDS             complement(30178..30315)
                     /locus_tag="PKM72_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59852.1"
                     /translation="MDQSQPHFTTVGQLIHTNPLRFDVATTCMEVAIALLSSQLSGGP
                     V"
     gene            complement(30556..31065)
                     /locus_tag="PKM72_03375"
     CDS             complement(30556..31065)
                     /locus_tag="PKM72_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59853.1"
                     /translation="MPPSLAFGGNGQQGQIARGLLVPGERIILGTVQLAMSNVIQLTV
                     GHPEPLFLSLRTGAEKGFQSIQRGDQLTIVISGKNQYTDFHTADFPEESRALKGHLLQ
                     PLMGDCKWVVIQTESGINQTHEVDENTRHTVMNIPVRMPAVFLLNKDNIPIDVRFGNE
                     DVYFMPLAQ"
     gene            complement(31464..32876)
                     /locus_tag="PKM72_03380"
     CDS             complement(31464..32876)
                     /locus_tag="PKM72_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNP59854.1"
                     /translation="MPPLPLPTPRSSWIPSTIIRRFFPHHHLSAGQLVTLGAMGLIVA
                     GTFLLKTPLATTPGKPLSFLNALFTATSAVTVTGLIVADTEKDFTLFGKIVILTLIQL
                     GGLGYAMLATLLLIAVGQRIGLRNRMMVAEAFSSLDLAGLIRFVKTILIITFTLEGVG
                     ALILAARFSQDMPVLAALGHGIFHAISAFNNAGFSTFSRNLIPYGGDFIVNASITLLL
                     VLGGIGFIVFRDLLEFYQKKRYRIQTHTRLALLISIGLWVIGTIGFFLLEMNSPATLG
                     SQTPSAGAMVAAFHSGAARTAGFNTLDLSLLRPSTLYMLILLMIIGGSPGGTAGGIKT
                     TTFGIVCLSVWAVFRKRLDVEFHYRRMPSHLVLQALAIMFLAIGAVTTLTFFLAFIEN
                     KPFLALMFEVASALGTVGFSVGNGGVLSLSATLTDFGKVIILCSMLLGRFGPLLVGLV
                     SLTPPVRMLYRFPQAKVAIG"
     gene            complement(33104..33499)
                     /locus_tag="PKM72_03385"
     CDS             complement(33104..33499)
                     /locus_tag="PKM72_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59855.1"
                     /translation="MPSFSKHRTIILVENQPDLRWLLKLRLEINGYTCLEAGNGMEAL
                     SLIQQHPSIGPILSDDLMPGMNGLQLLQELRLNFMNRAIPFILITATWSDEFRHQVLS
                     EGAFAVLPKPYHHSDLLQLLEQGIFPQAA"
BASE COUNT         7646 a         8877 c         9068 g         8138 t
ORIGIN      
        1 aatttcctga acctgttgtt gaaaggttgc taaaacggtt ccgtcttggg tggtaatcgt
       61 gaccagtaat cgattctgtt cgcgttgttt gtgtatcacc gatccttcaa gtggatgcag
      121 ggattcattc aagaaatttg gaaaatagag gtccagatag cgggtgacta acagtggacc
      181 acttaagccg aggcctaacc taagaaaacc gccgccggca agacccatga tgtcatggtt
      241 tctgctccgt gcttggaggt tcctctttcc ggctttgtcg gtccgttggg gaagtgagcg
      301 gttctgtgat tggaggttcc ccttcccggg attcctgctc cgttggggtc ggtttctgct
      361 gtccacgaat aaatgtttcc aggcgactga gggcatcggc ggtcatatcg cgagcccctt
      421 ttgtcgcatg ttcggatttt tcgctgaggt cttccatttg acgacggaga tcgcgaatcc
      481 ctccgccggt tcgctgaaac gccagaattg caatcactaa cgcgacgaca gcaatgagta
      541 atgtgatcac ttccatgatg tcctccttgt cgttctcaaa acgacttcac cgataaatga
      601 aaagtctttg acgcattgcg accgaaccct ttctgattct gacatacttc aaggatgaaa
      661 gataagatga atgatctggt ttctcaaaat ctcaccgtgt ctctgatgtc tgtgagcgga
      721 aattttggtg aaacttggaa taatccgttt tggatctata tttatggctt tggctcaaac
      781 cgccacctgt cgatagccta caccatcgag ctaggacgat tacaccacat ccacctggcg
      841 aagtaacaat tcccctcgca tgcatgaagg gagatcaccg ttgaattcgt agtcatccgg
      901 ctggcatctg aaaccccaat tgaaagatcg tttccggggt aaattataag ccctcaggaa
      961 ttttcccata gacctggctg ctattagaga aggtgcatac ccgtaaactt tgaaagaatt
     1021 gagatgcgaa ccggtattga ataccatggt cgatcccgat accttggaac gctccgtagg
     1081 ggagagccca ttttgtaata aaatattagg actgacgaat gatgaactcg atttctactt
     1141 cggtgggctg agcaattttg aatcgccgct tatccatgct caagacggcg tggccacggc
     1201 taatgtaaga atagaccagc ttttgcttta gtggttcatt aaggattttc ctatgtctgc
     1261 gaactcaggt cttggcagag gaaatgccct tcgctgtggc cattgaagta gatgagttgt
     1321 atttctgcag tcgtagaaga ggaagcatgg acgaggggct gttgggaaaa tcccggcctt
     1381 tggtattttg aaatgtgggt ggcttgtcta taatcccatc attcccgata ccaaagctac
     1441 aatgtctcgg tattagggga aatggggatg ccgcgcatag tcgtgtctct tcccctaccc
     1501 atcgaaacct ttagtttgag ggccatgcac taccaatcga cgcagttttc cgaacgtaat
     1561 aattattcgg tgctttgcgg tgttttagtg aatggcttct gtgagcaatg gacccatgaa
     1621 aaaactttga acgatgggat gacggtgacg agtgtatagg gccttatggc aattcgataa
     1681 ggtgattcag aatgggaaga gttcggtctg tccggtatag ataggggaat gcactgttgg
     1741 aggtaagaga aaggaaacat gatgtctatg gcaaaaagat tgagggtgct tctggtggtt
     1801 ttgaccgtga tcttcacggg aatgggaacc gagtctgggc ttgccgagga aattcggcct
     1861 ttaccctcca ttctcagtga tgtggaaaga cacattggag agttgaccat caacctggat
     1921 gacatcagaa aacggataga ttttttgcaa aatctccctg tcacacatga tccagtcatt
     1981 caggaattac gagcccttga tcttcaagga tggacactgc accgggaaca atgggaattg
     2041 caactggaac atttgcgatt tgcggagacc atgctacgaa aagtgcaggg aatttccggt
     2101 gaaccgcatg agttaagggc tgcctggcgc ctccatgaag gccagtaccg gacgagcctt
     2161 gaaggctatc gccgacaacg ggatacgatc gaagaacgcc gcctgatcac agaagggcgc
     2221 atgtttgagc ggtacctccc atgagcgaac gtcatgccac caagcacatg cctgtgtggc
     2281 gagacatggt tttcataggg ctcctcggtt ccggaatatt catgaccgga tgtacgtccg
     2341 tcaacacgct tcttctcact tcccagacgt ttccacccaa agcctcatgg caggaagttg
     2401 aaattttaat tcgggagccc tcctgtgccc atatcgacat tgcacaattg agtatggaag
     2461 gtagtgggtc gggcagttat gccaccgagc agaccgatat tcttaaaaag gcggcttcat
     2521 taggcagtga tgcggtcgtg tttaccaaat ctgaaaaata tttggagcat ggagtcagct
     2581 accaaagccc catgatgatg ggaggaccct ggggtatggg aagttatggg tatcctggtt
     2641 ggggttacgg aatgggttac ggtagaggca tggcgattcc ctacaattat actgtcacgt
     2701 cactaaccgg aatcgccata cgatatacga ataatgcgca gactaaatgc taatgtcttg
     2761 agaagataat tccagaaatg ttagtttcct ggccctgatg gggctcaatc cggaatagaa
     2821 acaacggcaa ataaagcatt gccaacccgt tgatatgggc acaatcatac tcagtatttt
     2881 ggagaaaata attgtcgtag cattgaggga gcgatatcgt gaacaggttt gaggctctta
     2941 cctattacat gtccacatta aggatgccat gtaacctccc tattgcattt tttttcatgg
     3001 catcaactgt ggcctgatat attttggatt ccgatgagtc atcaattcga ttttgatctc
     3061 ttatgcatcg gcagcggccc ggcaggccaa cgggctgcga tccaagccgc taagttcagg
     3121 aagcgggtgg gagtggttga acgtcgccag tgcgcgggtg gggtatgtat agaactcggg
     3181 accattccca gcaaaactct acgcgaggcc gtgttatcct tcatggctac caaccatcca
     3241 tttgtccaac agacaagaaa tcgggaacgg caccgtccca cagcggaaca actcttttcc
     3301 cgtgtgcaat ttgtccgcaa ccgggaagtc gatgttgtgg aggaccagct ttggaggaat
     3361 gatatcgaac tcatttccgg ggaagccgcc tttaccgatg cccataccct cattattcac
     3421 acagaacaag gaacgaaaac ggtcactgct gagaatattc tgcttgccgt cggaaccgta
     3481 gccgccccgc cgcctggtac cggcgtggat ggtgaaatca ttgttaccag tgatgagatg
     3541 gctcacctca catctctgcc caaacgatta gtggttgtcg gagggggcgt catcggaatg
     3601 gaatatgcct ccatgtttgc cgcactcgaa atcgatgtaa ccgtggtgga caaacgagag
     3661 catttactgg aattcctcga caatgaaatc gttgaagaac tcattcatca aatgagaaac
     3721 cgggatgtga ccttccgatt gggagaaacc gtgggcgcat tgaccattgc tgaaggcccg
     3781 cccaagcaag cggtgatcca actggagtcc gggaagcgcc tcgtgtccga catggttttg
     3841 tattccgccg ggagaatcgg cgcaaccgac cgattgaatt tggcgggagc gggtctgacc
     3901 gcggatgctc gggggcgcct cgcggttgat caagattttc gaacccaggt cccccatatc
     3961 tttgccgccg gggacgtgat tgggttcccc agtctcgcct ccacctctgc cgcgcagggc
     4021 cgacgggcgg cgtgccatgc ctttggagtc agtgccggtg cgatggatac ccactttccc
     4081 ttcggcatct acgccattcc ggagatttct atgataggcg cgcatgaaca tgagctgacg
     4141 gagaaaaaaa tcccttatga aaccggcgtg gcccgctata aggaaatcgc ccgtgggcaa
     4201 attatggggg atgacaatgg tttgcttaag atgctgtttc accgggagac ccggaagctt
     4261 ctcggcgtcc atgtgatcgg tacgggtgcc acggaactca tccatatcgg gcaagcggta
     4321 ctggaattag ggggtgggct ggactatttt cttgaccgcg tcttcaatta tccgacactg
     4381 gccgaatgtt acaaggtggc cgccttaaat gcctttaata aactttcaca aataggttag
     4441 ggtcttgagt cgaccagaaa ttgaagtcga caatgatatt cagaaaatcc accaaagcaa
     4501 gatcaatgcc ttttttatga ttcctgaaaa aggaatcagg aaaacctttt aaatcccaca
     4561 ttttcacccg actgaagatt tctctcacat ttcctgtcca acagcctaag ccgccgctcc
     4621 gaattcttca tgattggcgg ttcaaaactc tctaaaggat gagatggcta cgacttgaca
     4681 cacatatcgt gtgcgcatct tgccaaggct caggcggatg tcgaggccat ccaatttgtt
     4741 aggtattttg ctattcgcta attcaatttg ataagtattt ccctgtttat tatttcccta
     4801 gaggacgcga gaggaggtgt tttggttgga agatggtatc tctcttgggt cagcaaagca
     4861 tcaatgatga aaaattgaaa ggcttttaat agagtgtacg catgaaccaa cttgtcctta
     4921 ttgccctccg aaggccttta accttcgtcg ttcttgccat cttaatcgtg atattcggga
     4981 ccaagtcggt gctccaaacg ccgaccgatg tctttccgaa tatcaggatt cccgtcatct
     5041 ccgtcgtctg gtcctatgca ggcttgctgc cgagtgatgt gtccggtcgt atcacctttt
     5101 actttgaacg ggccttgacc tcgaccgtcg agggcatcaa gaagattgac agcaagtcct
     5161 atttcggcat cagcatcatt aatatttttc ttcaggaggg agtcaacctt gccggcgccg
     5221 aagccgaggt tgcggcgatt gcgcagacgg tcgtgaaagc gctgccacca gatatttccc
     5281 caccaatgat catgcgtctg gaagcgtcct ccgtgccggt cgcgatgctg caggtcacct
     5341 cagacacatt gacacccgca gaactctata accttgcctt tacccaaatc cgtccccttc
     5401 ttgtgacgat tcccggggcc atcttgcctc atccatatgg gggacaaccg aaacagcttc
     5461 tggtctcgct tgatcagcag aaattactcg cccgtaacct taccccagca gatattttcc
     5521 atgctttcga caggcagaac aaggtgctgc cagctggcga ccaaaagatt aaggccaccg
     5581 actggatggt ccagacaaac gcgatgccgt tgcaggtcga ggattttaac gatattccga
     5641 tcaagagaga gggaaacgcc ttcatttata tgcgtgatgt ggccaacgtg accctcgccg
     5701 gccccccaca aacgaatgcc gtgctggtgg aaggcaaaca ggccgtcacc ctcgtcgtca
     5761 tgaagagcgg ggaggcttcg acgttggagg tggtcgatgg ggtcaagaag gcgattcctc
     5821 gcattgaaaa gatcgtgccc aagggcgtag aggtaaaaat tctcaacgac gcctcgctgt
     5881 tcgtgaagga atcgatcgca gatgtggtga aagaaatggc gacggcttcc gcgctggtgg
     5941 cccttatcgt cctgatgttg ctgggctcat ggcggccgac ggtgattatc gccacctcga
     6001 tcccgctctc catcctgtcc gcactcatct gcttaaactt agcaggagag tcgctgaatc
     6061 tcatgacgtt aggaggattg gctctggccg tcggcatcct cgtcgacgac gcgacggtga
     6121 tgattgagaa tatcgatacc catattgcca tgggcaaacc ccttgaggag gcgatcgtgg
     6181 acgccgccaa ccagattatc gtccccacgt tggttgccac gttaagcatc gcgatcgtct
     6241 ggctcccgct ctttgaactc agcggcgtat ccggctggct gtttgcacca atggcaaagg
     6301 cggtcatttt tgccatgctc tcctcgttca tcttgtcgcg tacgctggtg ccaaccatgg
     6361 caaaatatat cctcgcggat caccacgcgc cgcaggccaa tgaagccggg ggaggaggcg
     6421 aaccggcaaa accccaaagc ttcttcgtcc gcttccagca gggttttgaa cgcggatttg
     6481 accggttccg tgagcgctat aatgcccgtc tcgaacaggt aatcctccat cgtcgcacct
     6541 tcgtcgccat ctctctcgcc ctcgcgcttg cctcccttag cctgtttttc atcaacgggc
     6601 gggatttctt ccctgaggtc aagtcgggga cgatgcaaat gcatatgagg gcaccgcttg
     6661 gtacgcgtat tgaggtctcc agccgtctca gttctcttgt ttcgaaagac atcgcgcaga
     6721 tgcttcccgg gcaggttgaa ggggtcgtca gcaattgcgg cctgccggtc ggaccgcata
     6781 acctcgcttt tattccgacg ccgacgatcg ggtcgcagga ttgcgacctg acgatcgacc
     6841 tgaaaaatga aaagtcaccg gtttgggact atcggcgtat tctccgcaaa ggtctaagtg
     6901 agcggtatcc aggaaccgaa ttcacgtttc agccggctga tctgaccgcc aaaattctca
     6961 attttggctc gcctgcgccg attgatgtgc agattaatgg gatggatatg tacgccaact
     7021 acgagttcgc acgaaaattg gcaggagagc ttcgcaagat tcccggcgcc agggacgtga
     7081 cgatacagca gacgatgcgc acgccgaccc ttttcgttga aggccaacgc acgtttggtc
     7141 tcggcacaaa cctgagcgag ggggaaatcg ccgacaatct gctgctgtcc acctcgggta
     7201 gtcaacagat tgaccagcaa tattggctta atcacaaaac gggtatctcc tatcagatca
     7261 acatttatac gccccagcct cagctcacga gcattaaaaa tctcttgacg gtccctgtcg
     7321 atcagaacaa tctcgactcg tcgcaagatg acgtacagct actcggcaat gtgacgacgc
     7381 attcaatgat tggaacgcca ggcctgattt cgcacgcgaa cattatgccg ctgtttaata
     7441 tctacgtgtc tgccgaaggg cgcgatctcg gaggtgttct ggcggatgtc gagaaggctg
     7501 ccaaaagtct ggaggatgag ttgccacgca gtgcagcgct tgaaattcat gggcaagccg
     7561 aaaccatgta tgaggcgtat gtcgagttga ttgtcggtct catctttgcc gtcgtgctgg
     7621 tgtatctcct gatcgttgtc aactttcaat cctggctgga tccctttatc attattaccg
     7681 cattgccagg ggctttggcg ggtattgcat gggcattatt cctgacccat acgaatattt
     7741 ccgttccagc gctgacgggg gccattatga cgatgggcac agcaaccgcc aattctattc
     7801 ttatcgtatc ttacgcccgc gaacggctcg aaatgcacgg agacgcgatg ctggcggctg
     7861 tcgaagccgg gaaagcccgt atccgtccgg tgctgatgac ggcatcggcc atgatcatcg
     7921 gcatgttgcc gatgtcgatg ggcaattccc ccaatgcgcc gttgggtcgg gctgtcatcg
     7981 gcggtctgat ggtcgcgacc gtgttcacct tgtttttcgt gccttgcgtg tatgccatca
     8041 tctacaacag gcgaggggct cgccaaaagg accatggata atatgaagat cctgcgtgga
     8101 agaggccttg tgatcttcgc aattctgctg agccttctat acatgggcta tcgaatctat
     8161 gaacacaaca gcggcgccgc tatgctgcgc gaggagacac ttgcagacgc cgttccaatc
     8221 gtggccatca tcaatcccaa acccttacca ccaacccaga ccattactct accaggcaat
     8281 attcaaggtt ggtttgaggc gccgatctac gcgcaggtct cgggctatgt gaaaatgtgg
     8341 tacacggatt acggagcgaa ggtgaagaaa ggcgacattc tggccaaaat caacgcgccg
     8401 gctcttgacg ctcaatatcg gcaggccaag gcggatctgg cgtccgagcg cgccttatat
     8461 ggtcttgcca aattgacagc aaaacgttat ctggcgctcc gcgaaaaaca ttccgtctcc
     8521 gaacagtcga tttcagtaaa agtggcagag gcgaacgccc aagcggcaag ggtcaatgca
     8581 gcggagcaga atgtcaaaaa ttttgaggcg ctgattcggt ttaagaccat cgtggcgccc
     8641 ttcgatggtg tggtgattga tcgcaacatc aatgtcggtg actatgttaa taaagaaggc
     8701 acgatcagtt ctcccactgc gatcagcaac ctctttactg tcgccgacgt cagcaaggtg
     8761 cgcctcttta tctctgtgcc ggaatctttt ggcgcattcc tcaaaccagg tctgactgct
     8821 gaagtaaccg tgccgcaatt acccagtcgt cgttttactg ccaaattcct taccgtggca
     8881 ggtggattca atgtgagcac acgcaccgct gttaccgaat tcgtgctgga caacgaggat
     8941 agggctctct ggcctggctc atatgccaag gtaagtctca cgacgcctgt tcattcggat
     9001 tttctcacaa ttccaaccag cgcgatggtg ttccaggaga atggcaccga agtagccatg
     9061 ttgacggatg aggatcgcat tcactttaaa tcgatcgccg tgggaaaaat cctcgacgcc
     9121 acggttgagg tcacagaggg gattacctcc accgaccgca ttgtgaataa tcccagtgcg
     9181 gcattgctcg aaaacgaaaa agtgcgcatc gtcacaccag cagctggtta tgaccgcctc
     9241 aacaccgatg gccccgcggc aaaggaggtt tctgtgccaa tggacaatcc tgcgccaacg
     9301 gaggcggcgg cctcaacgga ataatttcca ccatgatcgt ccctatacac tcaatgggcg
     9361 agcgcttaca tatgagacta gaaggatcac gcatctttcg ggcttgggcg tggcgcagca
     9421 gttccttact ccttgtgctc aatttgtcgg cttgcttgcc gcacgttgat ctggcgccgg
     9481 actatcaacc ggccgaattt gtcgtccccg tttcatggca tggtgccagt cccttcgtcg
     9541 aggcaaaacc atcggatggt gaattacgac gggactggtg gacgctgtat aacgatccgg
     9601 tattgaatac gcttgaagag caggccatgg tggccaatcc tgatttgcag gcggccgctg
     9661 agcggttcgt ccaggctcgc gatatgatga tgaaagcccg gtcacgtcgc atccctcaag
     9721 tgggcctggg attcggcacc tcccgcaatc ggcaatccga caatcatctg tttcgcgatc
     9781 ccgatatttc ggacaccgca tcgacggtct ctattggagg gattgcatca tgggaacccg
     9841 atttctggtc ggcgatccgg aacgccacgc gtatagagat gtaccgggcc gaagaacgtg
     9901 ccgctgaata tgggcttgcg cgactcagtt tacaggcgga aattgcggca aactatttca
     9961 cgcttcgcgg cttcgacgcg cagcttgcga tttataccaa atcaatcgac ctctataagg
    10021 gctcgctgga actgatcacg aaccagtaca ccgtcggaat cgcgtcacaa cttgacattg
    10081 cccgtgtcga atcgctgctg ttcagcactc agacgaaaga agccgaggtt cgaggccagc
    10141 gccaggtgac ggagcaggcg atcgccattc ttctcaatag ggcgccggct agtttcacga
    10201 tcgacccggt cgccgacctt cgtgtggaaa atttcacgat cccgaaaacc attccctcca
    10261 ccttgctgga acgacgaccc gatatcgctc ggatggagcg ccggatggcc caggccaatc
    10321 gcgcaatcgg catcgctcgc gccgcctttt atcctaatgt gacgtttctt gccggcgggg
    10381 ggttcgaaga ctcgggcttc gatctgatca agatggccaa tagtttctgg tcctatgggt
    10441 ccgccattga gctaccgctc ttccagggtg gatttcgccg cgcccaattg cagcagtcct
    10501 ggtccgttta tcgcgagacg gaagaccgat accgctcgac agtgctcaac gcctttcgcg
    10561 aagtcgagaa caatttgagc ctgaccaacc agctcaccac tgcagccaaa cgacaagacg
    10621 cggccgtcgg ggcctttctc aaaacgcaag acctgacctc tagcctctac tttggcggac
    10681 tggcctccag ccttgagctc atttacgccg aggtcaacac gctagcggcg cgtatcgacg
    10741 ctgtggtaat caaggccgac ctgctgaaat cttcggtagc cctcattcgt gcgctgggcg
    10801 ggggctggaa ccgcaatcaa ttacccgccg atgaacagat ccagcccttc gatacgttcc
    10861 aatatgttga tcttgacaaa cccccacccg ccgggggaat cgatgtcaac gcggacaata
    10921 actcggtgaa taatgatttg accaaacctt ccattcctta accggcattc aacccatgtt
    10981 gactcggcta tattggagag ccatggccgc attgaacgtc cctttctttg gaagattcga
    11041 ataaacaatg agctatcgga aaatcatggc catgtccgat attttcccgc acaaggtccg
    11101 aacatgtttg gcaagctgtt tgcgtaaagg gggtggtttc cgggccaccc ctcccggacg
    11161 ctccactcca cctgccggat ctgttacgat gacaacacag ataagggaga gaccagactc
    11221 ctagaagacg tttttcaccc ggccggttcc cgttccgagg cgtcaccgtt cagttcaaag
    11281 gaattttatg gccagtgcgc tactttacga tcgtctacaa tttgccttca ctgtcacgtt
    11341 tcattatctc tttccccaat tgacgatcgg gctggccttg ctccttcttt atctgaggag
    11401 ccgtgcactc gtttccggtg acgagcatta tcatcagatc gcccggttct ggacaaagat
    11461 cttcgccttg agttttgcct ttggtgtcgt gaccggtatt ccattggaat tccagttcgg
    11521 caccaattgg gcgaaattct ccaacttcgc cggcggagtg atcggccaga cactcgcgat
    11581 ggaaggcctg tttgcgtttt ttctggaatc gtcgtttctc ggcatcatgc tgttcggcga
    11641 agggcgattc agccggcggg tgcaatgggc cgcatcgctg atgctctttc tcgggtcctg
    11701 gctttccggt tattttattc tggcaacaaa ctcctggatg caacatccgg tcgcctatac
    11761 ggtggccccg gatggacggt tgttcgtcga cagtctttca agtctgctca cgaatccctg
    11821 gctgttctgg caattcgccc acaatatgac ggccgctgtc gtcacggcct cattcgtcat
    11881 ggcggcgatc ggttcgttct atctcctgtc cgggttgcat gtgaaggagg caaagacgtt
    11941 cctccgcacc ggcgtggtag cgggggccat cgccaccgcc ctgatgatct ttccgaccgg
    12001 ccacgaaaat gcccggcagg tgtttgaaca ccaaccggtc aaaggcgccg ccttcgaagg
    12061 gctcttcaag accgagcgcg gcgcgggtat ggtgctcatc ggtcagccga acatcgaaac
    12121 catgacaatc gacaatccac tcgtggtgcc tgatgcgttg agtattgtgc tctttgacga
    12181 actctatgcc gaggtaaaag gactcgatgc ttttccacgt tcagagtggc cggacaatgt
    12241 ggcgctgctt tattatgcct accatgtgat ggccgggctt ggaaccatac tcgtatcggt
    12301 tatgggactg gcgctgctct ggctttggcg gggacgattg tttaccgcga actggctgct
    12361 ctggctgctc atgcttgccg ccccgtttcc ttatctcgcc accacggccg gctggatgac
    12421 cgccgaactc ggccgccaac cctggctggt gtatggcctg ctccgcacgg ctgacggtgt
    12481 ctcaccgctg gtccattcag ggaatgcact gtttacatta ctcggatatc tcggaatcta
    12541 tctcgtattg ggattactct tcttattcct gatcgctgat ataatcaggc atggcccgga
    12601 aagttctatt cctaagtctg cgaacctata ggaaccggta caaatggaaa ccttctggta
    12661 tgttgccgtc acgttcatgc tggcggtcta tgttgtcctt gacggctttg acttcggcct
    12721 gggcatcgtc tatagcttcg tggcgaaaaa tgaaacggac cggcgtgcgg cgttgggcgc
    12781 catcggccct atttggaacg gcaacgaagt gtggctgatt gctgcgggtg gtctgttatt
    12841 tttcgcgttt cccaaggcct attcggcggg gttcagcggt ttctatctgg ccttgcatct
    12901 ggttttgtgg ctgctgattg cccgtggact cgcgctcgaa ctccggtcgc acgtgaatca
    12961 tcccctgtgg cggcagtttt gggatgtggc ctttgccggg gcgagccttc tcctggctgt
    13021 tgtgttcggt gtggccctag gtaacctgat tcgcggcgtg cccttgaatc ctgaaggcta
    13081 tttcttcgtg gcgctctgga caaatctcat gacaggaccg gagccgggga tactggattg
    13141 gtttaccatt ctcatcggag ccacaagcgc ggcgatcctc gccatgcacg gcgccaattt
    13201 cctggcgatg aaaacggagg gcgagttgcg aaaacgggcg cgacgtgctg cctggctgac
    13261 cggatcggcc gtggtaattc tggtcgttct tgccctgacc gccgttccct ttgtccaacc
    13321 cttattcttt ctcaactatg ccgcccatcc catggggtat gtctttcctg ttctcaccat
    13381 ggtgacattg ttgttggctc ttgaaatacg aaggcgtgat tgggatgcgg gagcattttc
    13441 cgcaacgagt ctcttgattc tgaccatgct ggccagcaca gcctgggggt catacccgaa
    13501 catcctgatt gcgacgagtg agccggcgaa cagcttgacc gtgaccaacg ccaccgccgg
    13561 cgtctatggc atgcagatcg gcctttggtg gtttctcgtt ggattcattc tgctacttgt
    13621 ctatcaagcc tgtgcacatc aggccttctg gggaaaggtc aagattgacg cagaataaat
    13681 ttttctgttc tgtatgatcc ctttccccgg actcgcgatc agggtctcta tcatcagcct
    13741 ctgagccaaa agtcgccact taccggttct tcgaaggtcc gccatacctg gaggaccata
    13801 tctccaaatg tgattccatg tgtaggacag taggcggggg atctccaagt actccgaact
    13861 tttcaacggt agacctcgtc attgagcgaa ttggtgatgt gctgcgctat gaccggcaga
    13921 cactgcttcc tgcccgagaa tgacatgagt catgaggcca cacagtttcg acggcctgag
    13981 agaatgacga atcgtaattt ccaaggcaac acgcttcccg attagcatac cgctgcctgt
    14041 cccgcctgtg ctgcctctgt attcaatcta agtcttgacg aaagtccaca ggcttccaga
    14101 gccccgatga atccgcttat attaaaagga gtagcagaag tactctggtt tctgggtcaa
    14161 agcgaacttt cttttttcct tcgatgaaca taaagtacgt tttctgcgaa gcaggcgtcg
    14221 gataacccac catcgcgacg agaggggaga atggtgtaac aggctcaggg ctcttcttca
    14281 gcatccccta aactggcctt tagggcatac gctcaattct ttctcccctc gtcatccatt
    14341 cgatgacacg gaatcaaagc catgatcaaa gccatatttg accaggagtt tttgtatgcg
    14401 aggactcatg atgaaatcaa aaaactcctc cgcgacattc agggatgcct ctcggcaagt
    14461 cgacacgacc gcttccccga acagaaccgg tatatgcatt ccggcaggat tttcatcaat
    14521 gatgcgcacg cttgcgttgt tgatcgcatc cacgcgatag atgatgccca tgtccgcctt
    14581 ccctgtgtga atcaggtcca cgatatcctc agcatgcgcc gcatggacca gcttgaaccg
    14641 gttcttatag gcgggatcac gtttggcaag tgcctgtgcc gtgatttctc ccaatgacga
    14701 cgttttcgga tcgccaaggg ccatgcggat cgctccattg ggcagcgcct cacgaaagga
    14761 gatcggcatg acgtgtgagc tcgctgacat gacgagtacc agagaggttt gtgcgtagac
    14821 ccgaggtcct ccattgagag tcagtttctt cttttgcagt ttttcgactt ccccaaccgc
    14881 cgcagggaga aacacatcga tcggagctcc cttttcaatt tgccgccgga atgtttgtga
    14941 cggaccgtac acgaccttta cggccgcatc atattctttc tcgaacatcg gcacaatttc
    15001 ccgtagtgcc gacctcaagc ttggggcggc cccaatggtc agagcttcag cctgaacagg
    15061 ctctccggtt gccacgatac cgaggatgcc aactaccagc gttatcgcca taccaagttt
    15121 ccgaattcct gtcatgatgc tcaactttcc gactctctcg ttagagggtc ttaccggtag
    15181 attgaataaa tgatgtctca gcagaacatc tgagtccgtt accatgccca tcgattcctt
    15241 ctttgatgac cgctgaagag gcgcataaac taaatgagat ttagtgcaaa attaataccc
    15301 cttacgcgaa acatatgatg gaacaaagaa agccttgaca acttaagcat tctgacgaat
    15361 tcctcgtgat ttcttctacc attgcctgat cccaacgtct tgccaaattg tgagataacg
    15421 cacggttcgc tcagtgcaga aatccacacc acaatcttta aaccttgacg ttcaacacgt
    15481 tcaacgtaga tctgaggtct acgacacgga gccaataacc cgctcgcatc ctgccatggc
    15541 gtactgaaaa gtggagcgca tttaacttgg tagggatttt gctagctcct ggtaatcgct
    15601 agggaagacg ttttgcacag ggaatgatcc tacgatcaca gaaaccaatg agttcatgta
    15661 ggtatatggt gtccaatatg gcagaaactc aaaccgtttt ttggaggctt cgcgcatgaa
    15721 tcagattgtc ctcattgccc tgcgaaggcc gtataccttc gtcgtcatga ccatcctgat
    15781 tgtccttttg ggggtcaaca ccgtgcgtca tatgccgacc gacatctttc cgaatatcac
    15841 cattcccgtc acctcggttg tctggaacta ttccggcatg ctcccgcagc aggtggaagg
    15901 gcgcatcacg tatctgttcg aacgcttttt gacctcgacg gttgaaggca tcaagtatat
    15961 ccatagccac tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggtcagga
    16081 catgcaggtc atggtcaacc tcgatcaaca gaaattgctt gcccgtcagc tcactccggc
    16141 tgacgttcac aatgccctcc aacagcaata cctcgtgctg ccttccggcg atgtgaagat
    16201 caaggagacc gactggatcg ttatgacgaa cgcgtcaccg ttaaagattg aggatttcgc
    16261 cgatttcccg atcaagcggg aaggtaacgc gtttgtctac ttgcgcgacg tcgccactgt
    16321 tcgtctcatg ggacgggtgc aacagaactc ggttctggtg aagggcaaac agaccgtcat
    16381 cctggtcgca atgaaaagca cggaggcctc gactctcgac gtcgtcgatg ggatcaagaa
    16441 aatgatcccg cgcgccgagc aaatctctcc ggagggcgtg gagatcaagc tcgtcaatga
    16501 cgcctcgacg ttcgtgaagg attcgatctc ggacgtcttg catgaaatgc tgatggccgg
    16561 gggcctggtt ggcctcatcg tgctgttgtt gctcggctca tggcgtgcga cggtcattgt
    16621 ctggacctcg attccgctgt ccattctgac cgcgatcatc ggccttcatc tgctcgaaga
    16681 aacgattaat gtcatgacct tgggcgggtt ggcgctggcc gtcggcattc tggtcgacga
    16741 tgcgaccgtg atgatcgaaa atatcgatcg ccatattgaa atgggcaagt tgcttgaaca
    16801 ggctatcatc gacgctgcca atgagatcgt tgtcccgacc ttcgttgcca ccctttgcat
    16861 cgcgatcgtc tggctcccgc tctttgaact cggcggggtt gccggctacc tgtttaagcc
    16921 catggcagag gcggtcatta ttgcgatgct cgcctccttt attttttcac gcaccctggt
    16981 gccgactatg gccaaatata tgctaaagga tcatcatgtc gcagccgcat gaacctcacg
    17041 gagagctcaa tgcggaagga tcgcggaata tttttgtccg ctttcagaat gggttcgaac
    17101 gcgccttcaa tcgatttcgc gggggctacg gtgcgctgct cgaacaggcg atggccaatc
    17161 gtggcgtctt cgttgcgacc gctctggcca tcgcggtgag ttccctgtcc cttttcttct
    17221 tcctcgggcg ggattacttt cccgagatca ggtcggacat catgacgatg catatgcggg
    17281 caccgcttgg gacgcgcatt gaggtttcag gacgcattgc cacccttgtc tccaacagca
    17341 tcaaggagtt gctgcccggt catgtcgaaa atatcgtgag taactgcggc ctgccggtcg
    17401 gaccgcacaa tctcgctttc attccgacgc cgacaatcgg ctcccaggat tgtgacctca
    17461 cgatcctcct gaatgatgaa aagtcaccgg tgtgggagta tcggaaaatt ctccgaaagg
    17521 gcctgaggga tcgctatcct ggaacggaat tcaccttcca gccgtccgat ctcaccgcaa
    17581 aaattctcaa cttcggcgcg cctgcgccga tcgatgtgca aatcaacggc ccggacatgt
    17641 accccaattt tgagtatgcc cgaaaactgg tgggcaagtt tcgcgaaatc cccggcgccg
    17701 cggacgtggt gatccagcag acaatggcca cgccgaccat tatggtcgaa ggcaatcgca
    17761 cattcggact cggggttaac aggtctctga aggatatggc cgacaacgta ctcatgacaa
    17821 ctgccgggag ccaacagacc gaccagatct attggctcga tcccagtacc ggcatgtcgt
    17881 atctgatcaa tgtctatacg ccgcagccgc agatcaacag cgtcaatagt ctcaagacca
    17941 tcccggtcca ggcctcaagc acttcttcta aggatccgga agggcaggag atgcagctcc
    18001 tcggcaattt agccgacatt accgcgacgg gcacgccggg ggtgatcacg catgggaaca
    18061 ttatgcccct gttcaacatc tatgtctctg ccgaaggacg agacctcggt ggtgtgctgg
    18121 cggatgtcga aaagattgcc aaggacatgg aggacgagat gcctcctacc gccgcgctcg
    18181 aaatccatgg ccaggcttcc ctgatgcacg acgcctattt cgagctgatc gtcggactgt
    18241 tcgccgcggt tgtgctgatc tatttgctga tcgtcgtcaa ctttcaatcc tggttggatc
    18301 cattcatcat cattacggcc ctgcccggcg cattggcggg cattgcgtgg gcattatttc
    18361 tgacccatac gcgtctttcg gaacccgcgc tgacgggcgc catcatgacc atgggcacgg
    18421 caaccgccaa ttcgatcctc gttgtgacct atgcgcgcga tcagctcaaa aagcacggcg
    18481 acgcgttacg ggcggcggtt gaagccggaa cgacccgctt ccgtccggtg cttatgacgg
    18541 cttcggccat gattttcggg atggtcccca tggcaacagg gtattctgcg aacgcgccgc
    18601 tgggtcgggc cgtcatcggc ggcttgctca tggccaccct cttcactctg tttttcgtgc
    18661 cctgcgtcta cgcaattgtt tacaacaggc gagcggctcg tcagaaggac catatacaaa
    18721 atgaataaga ccctacgtgg aaggctcatg gtcatctcag cggcacttct gtgtgtcctc
    18781 tacctcggct atcgggttta tgagagcaaa agcgacgcct ctttgctacg cgaggagacg
    18841 cacgaaaacg ccgtctccac cgtggcagtc atttacgcaa agccggtgcc gccaaccgag
    18901 accatcacgc ttcccggcaa tattcaggcc tggtttcagg cgcccattta cgcacaggtc
    18961 tcgggctatg tgaagatgtg gtacaaggac tacggcgccc tggtgaaaaa aggcgatatc
    19021 ctcgctgaaa tcaacgcgcc ggccctcgac gcacaatatg cacaggccaa agcggacttg
    19081 gaatctgtgc gcgccatcta tgccctcgcc gaaatcaccg caaagcgttg gctggccctg
    19141 cggaaaaacc acgcagtttc cgaacagtcg atctcggtaa aggtggcgga agcgaaagct
    19201 gaattggcga aggtcagggc agcagaacag aacgtcagga acttcgaggc gcagattcga
    19261 ttcaaaacca ttgtcgcgcc ctatgatggg gtggtaaccg tccgtaacat taatgtcggt
    19321 gattacgtga acaaagaagg cacgatcagt tcccccggtt cggtcagcaa tctctttact
    19381 gtggctgacg tgagtatgct gcgcctcttt gtctccgtgc cggaatcgtt cgggcccttc
    19441 ctccagcctg gcttgacggc cgacgtgact gtgccgcaat tgcccaatcg tcattttacc
    19501 ggaaaattcc tgaccgtcgc tcgtggattc gacgaaagca cgcgcaccgc gatcaccatc
    19561 ttcacgatcg aaaacgagga cagagccctc tggcctggct cttatgccca ggttcacctt
    19621 accgcgccgg tagacaggga agtgttcatg attccctcca ccgcattggt gttccaggag
    19681 cacggcaccc aattggccgt ggtgggagag gacgaccagg tccactttaa ccccattgtc
    19741 gtgagcaaac tcctggacag tgccgtcgaa gtggccgagg gggtttccac caccgaccgc
    19801 atcgtgaaca acccgagcgc cgcgttgctc gaaggggaca gggtgcgcat cgtgacgccg
    19861 gcgccgggtt atgaccaatt aaacgcgcca gctccaacga aggctgctgc gccaannnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccta tacagaggat gggccaacct
    20041 ttagacacga gaccggaagc ctcgcacatt tttcggagtc atccatggcg atttgctttg
    20101 ttactgctaa ccctcaattt gccggcgtgt agttggttcc cggctgtcga tctagcgccc
    20161 agctatgaac cgcctcaata cgtcgtcccc gcctcatgga agggggcaag tcccttcgtc
    20221 gaggcgaatc catcggatga ggaattacgc ccggattggt ggcaactttt taacgatcca
    20281 atcctgaaca gccttgaaga acaggcaatg gcagccaatc cggatttgca ggccgccgca
    20341 gaacggttcg ttcaggctcg cgatgtgatg atgagggcca ggtcgcagta ccttcctcac
    20401 gccggtctgg ggtttggcgc ttcgaacaat agacaatccg ataatagtct gtttcgcggt
    20461 cttggcgagg acaaccggga agtgtctatt accaccgggg ggctcgcgtc ctgggaaccg
    20521 gatttttggt cggcgcttcg caatgccacc cgtgccgaaa tctaccgggc cgaagagcgc
    20581 gccgcggagt atgggctcgc gcggctcagc ctgcaggcgg aaatcggggc gaactatttc
    20641 agcctccgag gattcgacgc acaggcggca atctataccc aatcgatcga cctctataga
    20701 aacgtgctca acctcgtcaa ggcccagttt gccggcgcaa tcgcgtcagc gctcgatgtc
    20761 gcccgcgtcg aatcgctgct gttcagcacg gaaacgaaat tagctcagat tcaaggccaa
    20821 cgccaagtga cggaacaggc gatcgccatt ctcgtcaaca tggcaccgag cagcttcacg
    20881 atcgaaccgg tcgatgatct tcgggtggcg gatttcgaaa ttcccaagac aatcccttcc
    20941 accttgctgg agcggcggcc cgacatcgcc ggaatggagc gccggatggc acaggccaac
    21001 cgggtcatcg gcattgcccg cgcggctttt ttccccgatg tccggttctc ggccggcggc
    21061 gggttcgaag ataccgggtt cagtctgatc aagctcgcca gtagtttctg gtcttacggg
    21121 tccactgttt cgttgcccct ctttcagggt ggatatcgcc gcgctcaatt gcagcaggcc
    21181 tggtcggcct atcgcgagac ggaagatcgc taccgttcga cggtgctgaa cgcctttcgc
    21241 gaggtcgaga acaatttgag cctgaccaac cggctgacgc tggcggccaa tcggcaggga
    21301 gccgccgtgg gagccacact caagacgcaa aatctgacga cggagctcta taggggcggg
    21361 ctggcgtcca gccttgaact catttatgcg caggtcgcca cactcgaggc gcgtatcgac
    21421 ttggtagcta tcaaggctga actgctgaga gcctcggtgg ccctcgtccg cgcgctcgga
    21481 ggaggctgga accgcaacca attacccact gatgaggaga tccaaccctt tggtacgttc
    21541 caatatgtcg atcttgacaa accatctccc gccgggggga tcgatgtaaa cgcgggcaat
    21601 aacggggtga ataatgattt gaccgtacct ttcgttcctt aaccggtgcc caccaaggat
    21661 ttgctgatat ttgtctttta ccgaaataat tcattcgcgt cctcctccgc ttggaagact
    21721 catacgagaa atcctaccgt gtgatgaaaa attcaatgag aaactccaag ggtttccagg
    21781 agaataaaaa ctggccgtcc aaagcatggg tgacctcctt gagtgggatt acgggcagtt
    21841 ttggcatgga atcctcttct tcagcgtcga attcggatcc ctgaatgaat ggaaattttg
    21901 ccttcgcgga caggttgttt tcttggccgg tgattcgttg aggggcgaat atgccaatac
    21961 gtcatgggtg tatcctgata cctgaaaatt accagttatc tggcaactcc cgaatgccgg
    22021 attcttcggt tcacccggtt cttacattcc caagaggcat ggaagaggag agacgttttt
    22081 ctaagattag tgaaaattaa tggcccctta atctcccttt tatcccgggg ggctaggatg
    22141 tgcttgcaac atcatattga ggtggtcttc ttaactatca aggaggaatg tccatggtca
    22201 caaaaaatcg ctttctatct ttgacccgta tgaacgccaa taaggcaggt ctggctggca
    22261 tcggatttct ggctggcatt ctaataatgt tcgggtcttg gaatatgcca ttttattctc
    22321 atgcggcaaa ttcccagcca gtagcttcag catctattgg atctccacca aatatggaga
    22381 aaggagggtt tgctgacatt gcccaggcgg tgacacccgc agtggtcaat attacggttc
    22441 gaaaagaagc accggttcct atgtcgggag tgccctctga tcggatgaga gaattttttg
    22501 gtataccagg aatgccggag atgccaggaa tgccgaaaag gcgaggcggg cctcctgctc
    22561 cggaagcgca gggagccggt tccggtgtca ttatttccag tgatgggtat gtactgacca
    22621 acgaccatgt ggttgatggt gcaaatgaaa ttacggtgac tcttccggac aaacgagagt
    22681 ttaccggaaa agttgtgggc cttgaccctc agacagattt agcggtggtg aaagttgatg
    22741 ccacggatct tccatttgta ccttggggaa attcctcaag tcttcgtgta ggtgaatatg
    22801 ttctggcagt cggcaatccc tttggattga actccactgt caccatggga attatcagtg
    22861 cgttgggtcg agggggaatg gggattacac aatatgagga ttttatccaa accgatgccg
    22921 ccatcaatcc gggtaattcc ggcggagcgt tggtgaatac gcgaggagaa cttgtcggaa
    22981 taaatacggc aatcttttcg cagtccggcg gataccaggg cgtgggattt gcggttccca
    23041 ccaatcttgc caagccggta tacgagagtt taatcagcac cggcacggtt gtcagagggt
    23101 tcctgggagt cggtattcaa gaaatgaatg ccgatctcgc acagtccttt caattagatg
    23161 gaacaaaagg ggctttggtt acgaatgtgg taccggggag tccggcagat gaagccggtc
    23221 ttcagcgtgg ggatgtggtt gttgaattcc agggtaaacc cgttttggat ccacgcagcc
    23281 tgcagcatca ggttatccgg accacagtgg ggtctgaagt gagcatggtg gttatgcgaa
    23341 atggtcataa gaaatccgtg aagaccgtga ttcgtgaaca agataatccc actcaggtgg
    23401 cacaggccag ccctgtggag aaagaaggcc ccctagccgg ggtggctgtt caagatctgg
    23461 atagtggcat tgcacaacaa ttgggattgg ataaaaatgt caatggcgtg gtagtcatgg
    23521 atgtggccca gggaagctat gctgcacggg ccggactagc gcagggcgat gtgatcagtg
    23581 aaatcaaccg taagccagtc cgttctgaag aagatttcgt gaaggcggtg tcctacttga
    23641 atgaaaattc atccgcgttg atctttatac atcgcgggaa aggggcgttg tatttgaaca
    23701 ttaaagttta acccccaaag gtcgtggacg agtcggttta aaaaaggggg cccccttcat
    23761 cgaaggggcc ccctttttgt tgaaaaatat taagtatttc cgtctgagta aaatgaaatt
    23821 ctgtgcacgg cgtagtgccc caggataccg gcgagccgca atcggatagt ccaccagaaa
    23881 tgaagccatc aacagcaacc gcgggtcctt tatggtttca acactgtagt gtttcaaagc
    23941 tcccagacag gtcgcatagc cgaggtcatg tcgtcaccct ttaaaattat cccattagcg
    24001 attcgggctt tctcgctaaa ctcctacaca agatgatggt cgatgccaat tcgatcgtta
    24061 tcgagcagag tgttatcgac atccagcagg aagaccacat tgtccggcgc gttcatgctc
    24121 atgtctcctc ggataagggg ttgtgccagc ctccgtctgt tgcaatgagg gcatccgcct
    24181 ctttcggccc ccaggtgcct cgcttataag gccggaccgg gtggtgcttt cccagaaccg
    24241 gatcgacaac tttccaggcg gcttccaccg cgtcctcgcg ggtaaagagc gccccgtcgc
    24301 ctgccatggc attccctatc agtcgctcgt atggcttttc ctctcccgat tgttcttcca
    24361 gaagatagag ttcttgctga tcgccgatga actcctttcc cggccgcttg acgcgggcag
    24421 cgagggcgac agccgaactg ggggagagtc gaaaacgcag ataattgcct cgtccggctg
    24481 ccggtcttga atcgtcaaac agtcgttgcg gtggaggctt gagttccacg aggacctcgg
    24541 ctgccgttgt cgccaaacac tttcctgcac gcaggtacca cggcactcct tcccagcgcc
    24601 acgagtcgat gaagagtcgc agggcacaga atgtctccac gtcggagtct ttcgcgacgc
    24661 ccggttcctt ccggtaaccg gtgtactggc cgcgcaccac gtcgtccggc cgtaggggac
    24721 gcatggcttg aaacaccttc gctttttcgc tgtggacggc tcgatagccc tgataggcgg
    24781 gtggctccat ggccagcagt gagacgatct gaaagaggtg gttctgtatg acgtcgcgga
    24841 ggcatccggt actttcgtaa aatgccccgc gagccttcac gccgaaatct tcgcagaggg
    24901 taatctgcac gctgtctatg cagtcacggt tccagatcgg ctctaggaac gaattggcga
    24961 agcgaaaata gagaatattc atgatggctt ctttcccgag gtagtggtcg atacgaaaga
    25021 tcgagtcctc tggaaacacc gactgtgcca ttcggttgag tttctgtgct gaggccaagt
    25081 cgcgtccgaa gggcttttcg actatgaggc gcgcatcttt ggccaagtct gagccgccga
    25141 gtccctggat gaccgccgtg aagagcgagg gagggattgc cagataatgc gccggacgct
    25201 tggcattgcc cagagtcttt ttgagtgcct tgaaggtcac ggggtcgttg taatctccgc
    25261 ctacatactg gagaagggaa agcaggtggt ctaaagcctc cgggtcatcg atcttgccgg
    25321 cctgcttaat gctgtccgtt acatgtttac gcaattgcgc cagactccac ttcggagcgg
    25381 cgataccgac caccggaacg ttgagcagcc ctcgtttggt catcgcatag agggccggta
    25441 tgatcatctt gtgggcgagg tctccagtga ccccgaaaag gaccagggcg tcggactgct
    25501 ttggtcggct gttcgtcctg cccatttcag cttttctcct ttttctcatc atgtccgcca
    25561 aactgctttc gcatggcgga aaggatccga tcggcgtagt ccgcgttccc gcgagattca
    25621 aatcgcccga aaagagccgc gctgatcacc ggcgcgggca ctccctcgtc gaccgcagcc
    25681 gcgattgtcc accgcccttc tccggaatcc gacacgcgtc cggaaaaact tgtcagatcc
    25741 ggatcctcga ccagagcctc ggcggtgaga tcaagcagcc aggatgccac gacactgccc
    25801 cggcgccaga cctcggccac ctggccgatg tcgatctggt actgatacgc atcggggtcg
    25861 cgcaggggag tcgtctcggc atcatgctct tggtctcgta ggccgacgtc ggcatgcttg
    25921 agaatgttga gcccttcggc ataggccgcc atcaggccat actctattcc gttgtggacc
    25981 attttcacga aatgtcctgc accggtcggg ccgcaatgca ggtagccctg ctcgtcgcga
    26041 ccggatgcgc ctgtccggcc cggggtgcgg ggtgctgcct tgactcccgg cgcgatactg
    26101 ctgaatatcg ggtccagatg ttggacgatt ttttcctctc cgccgatcat caagcagtag
    26161 ccgcgttcga gtccgaaaat tccgccactt gtgccgcagt cgacatagtg aatgccgcgg
    26221 cttttgagcg ccttcgctcg ctcaagatcg tcgcggtagt aggtgttgcc gccgtcgatg
    26281 atgatatcgc ccgtctccat gcgtgaggcc agttccttga ctgtctcgcc ggccacgccg
    26341 gcgggcacca tcacccaggc cgcgcgcggt ttggatagtt tcgcgatgaa ctcgtcaagc
    26401 gaggcggccc cctggacatc ccggccctcg agtttcttta cctcagcgct gttcctatcg
    26461 tagaccacgc atgtgtggcc ggcttttgtc aggcgccgga ccaggttcgc ccccattcgt
    26521 ccaagaccga tcatgccaag ttgcattttg tctctccttt tttttaatgg ttatccgtgt
    26581 gtgatcactt catcgtgggg ttccagaaga gccagggccc gcctgcatac gttagcgacg
    26641 gtgaagccat attcctgcat gaccatcttc cccggggccg atgtgccaaa acgatctacg
    26701 gcgagcacat caccattgtt gccgacatag cgataccaac cttgtgatac tccggcttcg
    26761 acggcgagcc tcgcgccgac caccggcgga agcacggtgt tacggtactc ttctgactgg
    26821 gcttcgaata gttcccaact gggcattgaa acgatgcgca cctggagttt ttgttccagc
    26881 agtttctccc gcgcctccac aatcaatgcg acttcagagc cgctggcaat cagaatcagg
    26941 tcaggatgcc cattcggggc ctcggccagg atgtatcccc cacgcttcag gccatcggca
    27001 gcggagagct gtgcacggtc gagcgttggc acagattgtc gtgtcaggat taatgccacg
    27061 ggccgatccc gcgtttcgat cgccacccgc caggccatgg ccgtctcgtt ggcatcggcg
    27121 ggacggatca cgatgagctt gggaatcgct cgcagacagg cgatctgctc cacggattga
    27181 tgggttgggc cgtcctcacc cagtgcgagg ctgtcatgtg tgaatacata caccacttga
    27241 atccccatca gggcagcgag ccggatcggc gggcgcatgt aatcggagaa ggtcaaaaac
    27301 gttgccccga atgggatgat ccctccgtgc gtagcgaggc cgttcaggat cgcccccatt
    27361 gcatgctccc ggaccccgaa ctggagattc cggccggcat agctccagcc gccgccggca
    27421 gaaccttgca gatccccgac cgccatgtcg gagggttcaa aattgcctgc atccttcaat
    27481 tcagtatacg tggagggatt caagtcggcc gagccgccaa tcaaggcggg gagtgtccgg
    27541 ttcaccgcgt ccataatttt tcccgaggcc tcgcgggtgg ccaggccttt gccatcggcg
    27601 gggaattgcg ggatgtttgt gtcccagcct ggcggtaatt cccctttgat gagcaggcga
    27661 agttcgcccg caagctcggg gtattgggtc tcgtacgcct taaaggtggc attccattcc
    27721 gtctccgctt gacggccacg gtcaacggcc tggcgacaat gctgctcgac ttcttccggc
    27781 acaagaaatg gcgggtcaac aggccagccc aggttttctt tcgtcagctt cacttcttcc
    27841 tcgccgagcg gggaaccatg ggccgcaaat gtgccttgtt tgttaggaga accgtaacca
    27901 atatgagtcc gtacgaggat cagtgaggga cgctccgtct cctgacgtgc ctcttggata
    27961 gcgcgatcaa tggcgttcac gtcgttaccg tcctcgatcg attgggtatg ccagccataa
    28021 gcggcaaatc gttgggcgcg gtcctcggta aatgtgagct gtgttgatgc ggcgagcgtg
    28081 attcgattat cgtcgtacag atagatcagt ttgccgagtt tcaaatgacc ggcgagcgag
    28141 gccgtttcgg ccgccacgcc ttccatcagg tcgccgtcac tgaccagggc ataagtgaaa
    28201 tgacgaacga tctcaaatcc tggccgatta taccgggtgg ccagataggc ttcggcaatg
    28261 gccatgcccg ctgcattgcc gaaaccctga ccgagggggc cggtggtggt ttccacgccg
    28321 ggggtcattc ctctttcggg atgtccgggg gtgcggctgc cccattggcg aaactgtttg
    28381 agttgatcga gcggcagatc ataacccgtg agatatagca aactgtagag cagcatcgat
    28441 ccgtggcccg ccgagagaac aaatctgtcc cgattgaacc agtccggatt ggaggggcta
    28501 tgttgaagaa atcgtgtcca caggacatag gccatcgggg cggcacctag cgggaggcct
    28561 gggtggccgc tcttggcctt ttgcaccgca tctaccgaca aaaaacggat cgtattgatc
    28621 gacaattgat ccaactgctc ggaactggaa acggatgtag gtgctttatt catgataggt
    28681 actccttctt tcgttgagag aatacaacat ggccgttcct gtttattccc ccggccgcat
    28741 gggttttcaa gtgccagtat gcttgaaggc aatcggaaga aaggcctttc cgaagtctag
    28801 gtagcgggct cactttgttt ttcgtcgcat ggtgcatttc ttggaggcag aagtctgcca
    28861 tgatgttctt gacgatgatg ccacgatctc ctccggcttc ccatgtccat tcaccatgat
    28921 cttcgcgtgt acaccttttg gccaagaacc tcttcgttgt ctccctgttc gtatgttttc
    28981 tcatatggca gcgattttgt cataataccg aaatctggac atcctcccaa ccctattccg
    29041 ttcttgtcaa agctgtccat catttcaatt acacaatcat acctcaccat ctggtagccg
    29101 gctttgattt ccatgggaag aggatgggcg ctatatgggg gagcaacaga gagttccggt
    29161 ttgtctcata gcccagcatg gttggcgagc cgatgattcc ttccggcgtc aagacttcgt
    29221 taaaggggtc tggcgcatac tccgcttgat gatatgcgtt gaagacgaca tatcgtgtct
    29281 gtgggagcgg ccgaaacaag ttcaggatcg cggtcatggg aacgcctcat agttcccctg
    29341 gaggttcgga taaaggaaaa aatctataca ctgctgcgca tcagcaagaa catgcagaac
    29401 agattcatga cagggttgat ccaaagccgg agtggaaatc atcaggtgtc ctggtccatc
    29461 cctctctcta tctttcctat ctggctttta cgggtttctt gcgatttcat ttccttgtca
    29521 aaagagagtt ggattgacag agatccggtc tcctcgtgtt ctaataaatg atgggggggt
    29581 gcttttcatt gatgactaat ttcaatcggc ttggttgaag ggggcgctcg ggatcgcgag
    29641 tatagtgcga gaaatcctcc gacaactcaa ccttgctgag ggggatggaa aggtgttgtt
    29701 ttatatgtga tacctgagtt tcaatcgaga gatcaaccaa ttgctcgggg cagcccgtga
    29761 cttcggtgtt tagatcctga gcatccagga attgcaccgt cactcgctct tccgggttaa
    29821 gccaccgagt gatcgtggcc attttttcat cgttcgtcat gttgatcctc ttcacgtgtt
    29881 gacggctgtt ataggattat tcctcatcaa tccctaaatc cccccaagat accctcgcag
    29941 aaggtcatgt cgggttaagg ttttcgtgac aactccattt tcttcgacac agagattcag
    30001 gagtttcttc tctttcatga tgctgattgc atctttaatt gtcgtatcat cgtgaatgag
    30061 ataggcctct ttcgacataa tttgttgagt ggtgatcttc ctaagttcct gcgaatgatc
    30121 cagggctttc aatagatcaa actcactgac aaaacccaga taggctccgg tctcatctta
    30181 gacaggccca ccagacagtt gggacgacaa cagcgcaatg gccacttcca tacaggtggt
    30241 tgccacgtca aaccgtaagg gatttgtgtg gatgagctga ccaacggtcg tgaaatgggg
    30301 ttgtgactga tccatggttc ctccttaagc cggttagaaa ttattacgaa aattccggtg
    30361 gtagccaaat gatctgtgac ctcagcccca aaggccaaca tggaatgggg gattcgcttc
    30421 tcgaaagaaa agccaaaatc ttatggatat ccaagacttc ccggagagaa ttcacaagaa
    30481 acgaggcaac acaatcaaac cggagattct atagccttac cactaattat gaagcaggcg
    30541 gtggcgtttt ctcgatcatt gggccagggg catgaaatac acgtcttcat ttccgaacct
    30601 gacatcaatc ggaatattgt ccttatttag aagaaagacg gccggcattc tcaccggaat
    30661 gttcatgacg gtatgcctcg tattttcatc cacctcgtgg gtttggttga tcccactctc
    30721 cgtttgaatc acgacccact tacaatcacc catgaggggt tggagtaaat ggcccttaag
    30781 tgccctgctc tcctctggaa aatcagccgt atggaaatcg gtatattggt tcttgccact
    30841 gattacgatt gtaagttggt cgccccgttg aatcgattga aatcctttct ccgccccagt
    30901 tctcaacgag agaaacaatg gctccgggtg cccaaccgtg agttggatga cattcgacat
    30961 ggccagttgg acagtaccca ggatgatacg ttcacccggt accaacagcc cccgagctat
    31021 ctgtccctgt tggccatttc cgccaaatgc cagcgatggc ggcattttgc tcatggaaaa
    31081 taaaagataa tcatttcaat ataaaacaaa ttggttggtt catactttgt ggaacatgaa
    31141 gtaatgaatt ggttacacga cgtacgctta tttgaggatc aaaattcatc gaaggcattc
    31201 tctttagcca tcatgggatg cgcgaaaatg cactttaacc acggagcgaa atagctcaat
    31261 ttcaggaatt gtaatcttgt ttgatttgaa attagatttg aatgtgaccg gccacttgag
    31321 gcctggtcat cgcagatgaa aaacgctaag aatggtgaag gggaggctgg tttcaatatt
    31381 tatgggtttt tccggattgc agaacttgga ggaaatggct gaatgcgtgg gttgtgtgag
    31441 gagttttttc gaggcgtctc ttcctaacct atcgccactt tggcttgagg aaaacgataa
    31501 agcatgcgaa caggtggtgt gagggaaacg agcccgacca ggaggggtcc gaatcttcca
    31561 aggagcatgc tacatagaat aatgactttt ccgaaatccg ttaaggtcgc cgagagactg
    31621 agcacgcctc cgtttccgac ggaaaaccca accgttccca atgcggaggc cacttcaaac
    31681 atcagagcca aaaaaggttt attttcaata aaggcgagga aaaacgttaa tgtggtgacg
    31741 gctccaatgg ccaggaacat gatagccagg gcttgtagga cgagatggga aggcatccgc
    31801 cggtaatgaa actccacatc cagtcgtttt cggaatacgg cccagaccga taaacacaca
    31861 atgccgaatg tggtggtttt aattcctcca gcggttcctc ctggtgatcc cccgatgatc
    31921 attagcaaaa tcaacatata caatgtgctg gggcggagga ggctcagatc cagcgtattg
    31981 aatcctgcag tccgggccgc tcccgagtga aaggcggcaa ccatggctcc cgctgaggga
    32041 gtttgtgagc caagcgtggc aggactattc atttccaata ggaaaaatcc tatcgtacca
    32101 atcacccata aacctatcga aatgagtaag gccagtcttg tatgggtttg aatccgatac
    32161 cgctttttct ggtaaaattc caagaggtcg cggaagacaa taaacccgat gccgcctaga
    32221 accagaagga gggtgatgga ggcattcacg atgaaatcgc caccataggg gatcaggttt
    32281 cgagaaaatg tcgaaaaccc tgcattgtta aaggcagaaa tggcgtgaaa aataccatgc
    32341 cccaatgcgg ctaatacggg catgtcctgg gaaaaccgtg cggcgagtat caaggccccc
    32401 accccctcaa gagtaaaggt aatgatcaga attgttttca caaaacgaat caatccagcc
    32461 aaatccaatg aactgaacgc ctcggccacc atcatgcggt ttcgtaatcc aatgcgttgg
    32521 cccacggcga tgagaaggag cgtcgcgagc atcgcatacc ctaacccgcc taattggatg
    32581 agtgtcagaa tgacgatctt tccgaataag gtgaaatctt tttccgtgtc cgccacaatt
    32641 aggcccgtca cggtgacggc tgaggttgcc gtaaagaggg cgttgagaaa acttaatggc
    32701 ttgccgggag tcgtggccaa tggggttttc aacaagaagg ttcctgcgac gatgagtccc
    32761 atggcgccca gcgtgacgag ttggccggct gagaggtggt ggtggggaaa aaaccgccga
    32821 attatggtgc tcggaatcca agagcttcga ggagttggaa gtggcaatgg aggcattatg
    32881 gaagccaggg aagtggactt aattcactgg aggcgggggt aaaaaaccgt gggaaaactc
    32941 aggccgcgaa tctagagtga aattgtgcga atgggctcct ccgcaggttg tcttcatggg
    33001 cgacatgatt tccaggggga aacaagggga gaagagcata ttgcaagaaa accagtgagc
    33061 aatcttgtcc gaggtgtagt ttttctgtca agaggggcaa gcatcaggcg gcctgaggaa
    33121 aaattccttg ctcaagaagt tggaggaggt cactgtgatg atagggcttg ggcaggaccg
    33181 cgaacgctcc ttcactcagg acctgatgtc ggaactcatc ggaccaggtt gcggtaatca
    33241 gaatgaacgg aatggcccgg ttcataaaat ttagtcgaag ttcttgaaga agttgcagcc
    33301 cattcattcc tggcatcagg tcgtcagaca aaatcgggcc gatggagggg tgttgctgaa
    33361 tcaaggagag ggcctccatc ccattccctg cttccagaca tgtgtaccca ttgatttcaa
    33421 gcctgagttt cagcagccat ctgaggtctg gttgattctc aacgaggata atcgtccgat
    33481 gtttagaaaa agaaggcaat gagtcttata caaggatata catatcctgg gagaactcca
    33541 tgtgtaggac aacatatctg caagggatat tccagaatgc tcttcagagc ctattgtcat
    33601 atgggctaga gggcacccct ttatcaaacg gtttttacag atatttttgg aattgccgga
    33661 aaaaagattc cgggaagaac atgtgacggc ctaagaaagg gagaaatgtt gtctctatcg
    33721 gagacaatct gtcttttttc ttttggacag gtctcgctga aggttgctac gcgagaggaa
    33781 ttacttcctg aatggttaga gaacagatga agattgaggc ccctgccaat gtctgacagg
    33841 ggccgattcc ccgaacgggg aaaaatcgtg cttaggttca cgttacatga agtagtcgaa
    33901 ttcgctttca cgatgggtcc ggcggcgga
//
LOCUS       DAORAQ010000019        29813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_309_length_29813_cov_64.202702, whole genome shotgun sequence.
ACCESSION   DAORAQ010000019 DAORAQ010000000
VERSION     DAORAQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29813
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_29813_cov_64.202702"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKM72_03390"
     CDS             <1..557
                     /locus_tag="PKM72_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59856.1"
                     /translation="GMIAMLAFFISYYLFRSLGVLAFEGKEGFGGPQWLYDQVFVPVL
                     TLHILLVVIGLVMAIYMMVLGFRSQTFVEGKRVLKDAILKTSGRRIAMILGGVTTIVL
                     LFFLSRVMTSGFSMGKFSVYLGLILLIALVFGIEISIQRIWPNGARRHRVLGRFTMVI
                     YCVLFVTGTFTYTMLYILYPGKIG"
     gene            703..1044
                     /locus_tag="PKM72_03395"
     CDS             703..1044
                     /locus_tag="PKM72_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNP59857.1"
                     /translation="MVPHTLWSDEALYQLSRLPPYLLPLVREEVEEFASTRGQRVVTV
                     SRVGSARNKGMVEWSPDAERRINNVPSGIRAMARIELERTALDRGMPAVTVALMEEVK
                     ARYFGMAAGRG"
     gene            1111..2184
                     /locus_tag="PKM72_03400"
     CDS             1111..2184
                     /locus_tag="PKM72_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59858.1"
                     /translation="MLHRLSLRVLPQLNALVTDQPVRKRKRLVMLAPGLVAFGIYHLL
                     KPVLPLADPLVLLAFTGCICMLTAIWSYRQGRGVSLMESWREDGVKQWVWLVGWIGLV
                     YGIQLSLLVLAILQVFVAYDFLLHPEGPAMMATIISCTSVTRDAFEIGHVQRMRQQGV
                     VVPTFPDGEALRKWIPQEIGSIVKWGALATMIGIAGSWLLFTVGPIELQPILQSLLVP
                     VMIASIGMKTFFSGESLYAQNTQGSRPRTWLSSLWFWMWPNLTFSATYFLVLMGMAAF
                     VLRVEQISQVGFMGIAACTAMVMALYTLFLGWRKSYEEQLVGIPESIQRCPFVMGILQ
                     NSKSSEPLPSLADAKVGHGNLVN"
     gene            2186..4057
                     /locus_tag="PKM72_03405"
     CDS             2186..4057
                     /locus_tag="PKM72_03405"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNP59859.1"
                     /translation="MKNLFKPLLSVLFIFLMQSGIALGMDAYSLPATDGQASQDIFYK
                     TPGTIEGPSVAETHQDYSAYPLVDNRLVVWFVTQQHTYFGGFVLSIPLFCLLLEFIGI
                     TRKQPASQQKFDGLARDILRVALLSLSITALLGCLMLITFVTLYPGFMSYMGSTFKPV
                     MPVYATVFVSEAFLLALYYYTWDFLKTPAAKWVHMTLGVLSNATGVVLLLLANSWASF
                     MMAPAGVDAEGHFLGNVWHLLHSPLWNPLNTHRFLADIMSGGAVVVAYATYRYFMAKT
                     PEDRAYYDWVGHVFIVVVVCALLPMPLAGYWLMRSVFEFRQTMGMAMMGGMLSWLFVL
                     QAIMVGVLFLGINYYIWQSLARLQGGERFHPHFKAILFTLMVCFLVWFTPHTIAMSGS
                     EMKAMGGQQHPVLGNYGVMSAKNGAVNVMICLTALSYILYRRANYVMTVPWASKGNIF
                     LVGLFLMGIANIVWLAIYGFYIPANVRVGLSYPQGMTTATLVVGGLLLNRLLLRGAHM
                     NGPVHWGRITPRGIVALFVVAAAFTWVMGLMGYIRSVGRLEWHISEIMPDQSSWAFTP
                     SLGFAAKMVTLNMIVFWSSVFFVFWLSRRDQQVVERRTAEFARPSPVIQVISEEESA"
     gene            4057..4791
                     /locus_tag="PKM72_03410"
     CDS             4057..4791
                     /locus_tag="PKM72_03410"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNP59860.1"
                     /translation="MKVYHGQYNNLGNVKRVRARSSFLACLIVSILVYGSGAAWAQEV
                     SNTGSSSSSMVLEDFQHPDAKGFPVGWEAQRSTVTAHETYTIQEEDGTFFLSAKDANQ
                     RVYTKHMTWDPKQHPILTWRWRIQTVPDNADFLAAIYPSLDVDLMFIPVNTKYVWSAT
                     LPVGSVKEGGMFSSTEIVIRSGTESLGTWVEERVNVYEDFLRIHDHEPAPHAWGISLL
                     GGPGVEVDFGSIQVQAESSKGDLVRE"
     gene            4802..5176
                     /locus_tag="PKM72_03415"
     CDS             4802..5176
                     /locus_tag="PKM72_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59861.1"
                     /translation="MKITPAKIFDVLLGVLGLGTVGLLIGVFMGDGWLPVALALGAIL
                     GAGVGLVGGRGFFLSIFIGTILGGLLTLGVSGTEAVTVGAASGAAMGGFLGIWISMLI
                     ETWRQRNQTVPECDKHPNHLQP"
     gene            5227..6147
                     /locus_tag="PKM72_03420"
     CDS             5227..6147
                     /locus_tag="PKM72_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNP59862.1"
                     /translation="MENQRVLIGSILFVFGSFIMMIVMLIYMTYKGKADLAELSAGQP
                     ANVRVLQPMPTQDFSMYKTFVGDDGREMVEIPEGPFTMGIEDGDPDEGPSHPVYLQTF
                     YIDLKEVTQADYDRYIKMTKRDKPKVPVFEDDVSKLVGLDYPVVAVTWNDAFGYCQWA
                     GKRLPTEAEWEKAARGEGKRRYPWGEKFEYQFANVDGKEDGFQYLAPVGSFEVGRSPF
                     GLYDATGNVAEWVMDSYAADYYQQAPYRDPPGPKVEDENKVIRGGSWRESRIGARVTK
                     RFAAKMWRNDASVGFRCAKDPPATETPQSS"
     gene            6233..7105
                     /locus_tag="PKM72_03425"
     CDS             6233..7105
                     /locus_tag="PKM72_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNP59863.1"
                     /translation="MDGRFKLIFLIAVLFMTGLPLLGILKGTDTPPSLPDPTPQSMPS
                     SALEPSTESMVPDPDIGNEMVEIPAGEFILGSNEGGFNEKPAHVANLGTYWIDRYEVT
                     YKRYMEFVEATGHRQPGPPSRYAAKLGLLRGPYQPITYVSWSDANDYCQWKGKRLPTE
                     QEWEKAMRGTDGRTWPWGDGLTGHPANFAGEGDGFEVSAPVGSFPLDQSVFGVFDGAG
                     NVMEWTDNWYVEDLYLQKDSVSQENNRPTSTYKTMRGSGYTSRGVDLRITNRSFMVPD
                     FRDETIGFRCARSN"
     gene            7794..9761
                     /locus_tag="PKM72_03430"
     CDS             7794..9761
                     /locus_tag="PKM72_03430"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNP59864.1"
                     /translation="MGRLFNQMTRGKKKLFAVSALMVMGGLLLLPIFLSVSAVASGGG
                     APAGGPPPDVVAAQKAGEEGEAEKVEMGRDIYYKTEGPAIGEGAPVTADNETFYPRYN
                     FESRVLLWVANQQHLYYGSFVLAVPIFCMCIEFAGMVSKDKAMAKKYDQLAYDFIKIS
                     LTAYSLTAILGGILIFTFLTLYPAFFGYLSSIFRPVMHIYALTFVAESATLYIYYYGW
                     DKMREGVLKWVHLSMSVILNVIGTVLMFLANSWIAFMMSPAGVDEQGRYLGNIWHVIH
                     TALWNPLNVHRILGNMAFGGGVVAAYAAYRFLSSKTDEERAHYDWMGYIAMSLGVAFL
                     IPLPFAGYWLMREVYAYRQQMGITLMGGLLAWLFIIQATMIGILFLTTNYYLWQALGR
                     MTGGERFQRYIKYLVFILVVGLLVFITPHTIVMSPAELKAMGGQQHPVLGNYGVMSAK
                     NGGINAIIMTTILSFIWYQRGNRVPTVSWAKFGNIFMGCFFVIAQLNNVWLACYGYFI
                     PANVRIGLSVPQVAGTLSCLLLMTPLNLAMLKNGRQLGPIRWGQIPPRSQYAIIMLAT
                     AFTWMMGLMGYIRSSVRLFWHVNEVMRDNSPWAYTHTIGFAANVISFNVLFFWISIMF
                     VFWLGTLGAKKIPVPSPAGQPVPSPQSATGH"
     gene            9828..11141
                     /locus_tag="PKM72_03435"
     CDS             9828..11141
                     /locus_tag="PKM72_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNP59865.1"
                     /translation="MVELLGRAIGMGWPVLLMLVGLLLYFQATISDPEKKKRASFQTI
                     IGIFCAFLAFIAISNYTHNFEGESRLLPVSLVMITIMSFIMGLYFPNISALMKIGGFM
                     FFVAAALSGYGNWLPQVEGGFPPPVVKLDFQSMSPQQLGDEGEKIIFGGIGQSKTQGA
                     IGKGQCPLCHGFQQGFLSERAPNLFGIPERSEERLKEPNYHMNNAAARTTEQKEAFEG
                     SGTATNAQEYIAESHACPSCFVVTGFGVKGSNDTVSPMPKIHKPPISLTIGEMAAVDT
                     WMYTREGKEAPAYDVIATSYEKFIPEADRPSAGGEEEAGGGGGNLLADGSEPYDKLFM
                     KAGCPACHTIPGIEGATGKVGPLLMEGSNAPNRIKDPNYKGKAKSPKEYITESILDPS
                     AYVVKDFPDDQMPKDFGVRLTGGALSKMVDYLAQLKEGQPLPPKE"
     gene            11187..12155
                     /locus_tag="PKM72_03440"
     CDS             11187..12155
                     /locus_tag="PKM72_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP59866.1"
                     /translation="MTWLKLVEGYMPMQMISELAICILVFSIINYSLKRAGMGLPKFW
                     AGIFVWCFVNLFYLKYRIYPPIPFSVRAIYGTVAACGIFMWVSGSQQEWEEFKRPIIN
                     VLDAKTGLTKFVRTALLILLPIGLWGFAYNSFLPSFDEPIELRTVHPAPPATTKVHGK
                     TYVLQTAANPFRINTEGKYDQAYSNAHIVSQDMGRLMKDVKNPEDNPWDPNAKGYIKH
                     VREGGEIFFQNCHFCHGDNLNGRGLWAFAFNPIPANFTDAGTIAQLQETFVFWRVAKG
                     GIGLPGEGFPWASVMPPWEQHLTVDEIWKVIMFEYWHTGYYPRTWE"
     gene            12169..14043
                     /locus_tag="PKM72_03445"
     CDS             12169..14043
                     /locus_tag="PKM72_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNP59867.1"
                     /translation="MNGNKSQNGLFEKRMVMNETTTPAEYGRRGLGAWLLSCGLLIGG
                     LGLATTSHAEMAEGFAEGSRPAPPSAEQVEAGKRVYFTKCVWCHGVEGAGDGPGADRL
                     WPRPRNFNAGTFKIRHTASGELPLIDVDLFQTVTHGLPGSAMPSWEGILTEDQRRDVL
                     AFVTTELVKDRSWQDTEFEEFHVLQLDKIQPVAPSAESIKRGSELVKEMKCIECHGVE
                     GRGDGNAFNLKDDWGFSIQPADWHKCWNFRGSRQDAYNVKNIFRTFSTGVSGTPMPSF
                     ADNTTVEDRWHIANYVNHLCERDVDVDIAGGNVTDEIAAALLAAKPRGIDPLTDKPKV
                     DFVVPSKFVEGELPADEHDERWKLVDRRIVAMGGQITHKPRNFVTRIDDVWVQSLYNE
                     THISFMFRWDDRTKSVQQDSVDWEPYEVNLGDYGIEEQAPGGSKFADDPEHPESIAAK
                     QTAYQVFNDGLAFQFPIKWQELPAPRKPRYFWGDEGFPVDIAKWTADGELKAYQGEGW
                     DVDFEDRDDFTEELKTVKAEWKDGRWTVIITRPLKGDYEEDAYIELGKYIPINFFVWD
                     GHNGDVGRKMAVSAFYYLVLEPPIEKETYIYPTLAAIGLVLVEGWILTRRANRRKGKV
                     "
     gene            14051..14728
                     /locus_tag="PKM72_03450"
     CDS             14051..14728
                     /locus_tag="PKM72_03450"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNP59868.1"
                     /translation="MNFGNIRKNEGGGQPPPFFMTNIKKFTNISGVVLAGGKSRRMGK
                     DKRILEWEGVKFLDKVCLTLVELFDEIIVVTAIEDYPCGHLPVRSVTDAIPHTGSLGG
                     MFTGIKEASHSSVFVVACDMPFLNPSVISRLCALPDNDVVMANLSTGYQPLHARYSKR
                     CSLIMEKMIQDGNLRIHSLIQDSSLSVQIVEESLFDDIDPHGYSFLNINTPVDYEFAR
                     KAASHLK"
     gene            15881..17662
                     /gene="argS"
                     /locus_tag="PKM72_03455"
     CDS             15881..17662
                     /gene="argS"
                     /locus_tag="PKM72_03455"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNP59869.1"
                     /translation="MEKILQNQVIEAIAVILSTAKESGELMVDTIPTIVVEPPKRPEW
                     GDFSSSVAMTLASQARQPPLNVAELLAAGLRAQFSDLFVHVNVVPPGFLNLTLHPHRW
                     IQVLRMIQDKGSLYGTSDIGKGKRILLEFVSANPTGPLHVGHGRGAALGQAIASLLKS
                     TGFSVFREYYINDAGRQLQLLGRSVHARYREHWGKPSSFPEDGYHGDYIKIVAESVAK
                     THGQTLLDESSEKAETLCAQLASQMLLDRIKEDLATFGVEFETWFSETSLHKTGLIQQ
                     SLDELRQKRLLIEEEGAWWFRSSQFGDEKDRVAQKQDGSYTYLAADIAYHRQKLERGF
                     DTLINIWGADHHGYIPRMEATVQAFGFAKETLRIVLVQMVSLRRGGQKIEMSKRAGEF
                     VTLREVIDEVGPDAAKFFFLMRRADTHLDFDLDLAKQQSSDNPVYYVQYAHARLASLF
                     RVAQERQVRLPVIQDVKQALLIQDEELGLIKTLAQYPLVVESSALALEPHRVTFYLQE
                     LAAQLHAYYNKNRVLPSLDPERDTGGRGENIKFETSGNTGTLNQELRYESISPDLTAA
                     RLALLRQVQTVLGNGLSLLGISAPEKM"
     gene            17664..18869
                     /gene="tgt"
                     /locus_tag="PKM72_03460"
     CDS             17664..18869
                     /gene="tgt"
                     /locus_tag="PKM72_03460"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNP59870.1"
                     /translation="MREGQAFAKIMPQEPSISQSGPNRFHIQHTVPSGRARVGRLFTA
                     HGEIETPAFMPVGSQGTVKGLDPKEVRECGFHMILANAYHLFLRPGHELIESLGGLHA
                     FMQWSGAILTDSGGYQMVSLADLCKVTEEGVGFRSHIDGAWHLLSPEKSMAIQVALGS
                     DIMMVLDHCPTFPCTEQQAREAVQRTTRWAKRCAVVPRKEHQWLFGIVQGGVFTQLRE
                     ESTQELVNLNMDGYALGGLSLGEEKPAMLTMIETVIDQLPPSHPRYLMGVGLPEDIVE
                     GVGRGLDLFDCVIPTRHGRTGWLFTSTGKVLIKQARYSRDPNPIDAECGCPVCQRFSR
                     AYLRHLFLSHEMLGVRLNTVHNLWYYGSLMKELRQAISANRFDDYRRDFYRRRECDPS
                     ETLIQDVTT"
     gene            18911..19234
                     /gene="yajC"
                     /locus_tag="PKM72_03465"
     CDS             18911..19234
                     /gene="yajC"
                     /locus_tag="PKM72_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNP59871.1"
                     /translation="MNLDSYAWAQAGGAGPESSAGLLSLIPFLLIFVVFYFLLILPQQ
                     RRQKKQRELLDSLKKGDKVITSSGIWGTVTNLDKETATLQVADNTKIRLQRDHVASIR
                     TSNES"
     gene            19254..20888
                     /gene="secD"
                     /locus_tag="PKM72_03470"
     CDS             19254..20888
                     /gene="secD"
                     /locus_tag="PKM72_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNP59872.1"
                     /translation="MKKLRGRLFLLFVVTVVSAILALPSFPGLFQSLPDGVKRVLSHR
                     GLSLGLDLQGGIHLVLEVEEERAVEIAVDRIRKAVEDLLKDKAIEVEGVRREGSKMIV
                     ITLQQEADGEAVRTLLDESFPNFMSQNSAGTRLVYELRDTEEERVKTSAINQALETLR
                     NRIDEFGVAEPLIQRLGLNQIAIQLPGVKDPQRAKDLIQETALLEFKLLEESKAALDL
                     PPQVEKGQEDTVRKTLEGKIPEGAEILFETAVSEPDGRAYSIPYLVKKDAVLIGDVLQ
                     DARVSIGEFNEPIVSITFDSKGAREFDELTAANIGKRMAVVLDGKVYSAPVIRDRISG
                     GRAIIEGTFSTAEANDLAVVLRAGALPAPLKTLQDLTVGPSLGQDSIEKGLRTTIIAG
                     SLVLIFMIVYYRLSGVIANMAVFLNLICLLGALSGLNATLTLPGIAGIILTIGMGVDS
                     NVLIFERIREELRQGRPVRLAVDSGYNKAFLTIVDSHVTTLITGLALFLFGTGPIKGF
                     AVTLCLGIAINLFTALVGTKVVFDFMNRRKLDTLSI"
     gene            21047..21967
                     /gene="secF"
                     /locus_tag="PKM72_03475"
     CDS             21047..21967
                     /gene="secF"
                     /locus_tag="PKM72_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNP59873.1"
                     /translation="MMEILGKTDIDFMGKRNIAFMVSGFLVSLGLLAVIAILVGWANL
                     GIDFAGGTAVQLKFDKPLLIADARKALDAHGLGAAELQEFPQDQKLLIRVKTETTIEE
                     GVSKNIIQAFQAEFPDHGFVVDSSTSIGPTIGKKLQEDALIAVILSLAGIILYVAVRF
                     EFRFGVAAAIATFHDVLAVLGIYFLLNTEITLLVVTALLTLAGYSLTDTVIVFDRIRE
                     NLRQRRRETTTTMINNGINQVLSRTLITTLTVVLVLVPLTIWGGEVLHDFSLALLLGV
                     LIGTYSSIFVASPLLLLWPGAEGKLLGRGK"
     gene            22031..25255
                     /locus_tag="PKM72_03480"
     CDS             22031..25255
                     /locus_tag="PKM72_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP59874.1"
                     /translation="MLDVLYGFFIVAKVKSFGFPLQLKGKTILAMVLVGLLPLILSLL
                     LTYFEEKRALREAAGITMKGIAVEVARKVETQIIRGINEAQQLATIPFIRSAVLNSNR
                     SYADKNPDEIQALIQQWQESWRIQSSLDEFPVFLNKYATDYLIQWHAIRKSDYLAIVV
                     VDAQGALVLTSFPQVNFFHGNSLWWEAVVQHHETQAFVSDLSFDPEFGTHVLNVGVPI
                     WDDLRQHVVGAISILLRRDSLFRSISEVAAGKTGHAMLTASDGMPILCPAFSLEEHVM
                     PSNVVSAFQQGGVGWLVADPDSHGMPNAIVGYAPLHLGMPLAPESFGGKSWHMLTSQD
                     SAETYAPLDQLLSKVIFYGIAVFVILWGAGVVVAGRIVKPIQVLSQGVEQFGSGNLSE
                     KIAIRSGDEIERLADTFNAMAENLQHSFFELNQKVDEIGRLEQKYRDLIENAPEMIHQ
                     LDPAGRFVHVNTTELQKLGYSLAEMLEMSLWDIVPAEQQEVVQAYVQGLALGGARSIE
                     TVFRTQSREVMDVEIHSTTLMDPGTHLVVYSRGFVRDITARKALEREVERYTNQLEGI
                     VAERTQQLSESEAGYKALFNLAADSIFVVDSEGRILDVNARERDILGYVPSDLEGASF
                     VKLVPPAFQVVSQSLLDRVSGEEPKVPTTEIEVYDRQGVMKSVEMDLVRIDMGGRASI
                     MVQLRDITEHKKFEAELQRYNEVLEEKVSERTREIEQAKLYIESLLENANDVIYTLDV
                     DLRFTYVNGKVDAWGYRKEDLIGKPYLSLLSKRYRGRYLKETLDLGTKQVYEVEVVSR
                     EGELRAVMVSVAPLLNDAGVYTGVLGIARDITERKHLERQVQNSERLASIGKLAAGVA
                     HEINNPLGGILNCLYNIRKGTLTPERSQEYLHFMEDGLRRVQRIVRQLLDFSQQREPE
                     LAMTDLHQILDRVLVLTEHVFLVKHATLSKFYDETLPLVMVDAHMIEQVIMNLVLNAV
                     QALKEGGQVTLRTRKQEDGYEIEVEDTGCGIPPEIRPHIFDPFFTTKGTGEGTGLGLS
                     VSLGIVQRHAGEMLVDSEEGKGTRFTIRLPLVRSRSGIPVKVGT"
     gene            25252..26712
                     /locus_tag="PKM72_03485"
     CDS             25252..26712
                     /locus_tag="PKM72_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP59875.1"
                     /translation="MTHGSILIIDDEPLMRVSMVDALKAIGYVVQEASSGLEGLERLR
                     TSRFNLVITDLRLPGVDGLHIVKQCKEHCPGTEVIVITAHGSVDTAVDAMKSGAYDYI
                     TKPFSMDELLLSVERVCAVVALREENRVLRDELEKKFSFQGIVGKNERMRQVLEKIKL
                     VSVTDATVLVVGESGTGKELIANAIHANSARRNHALVKVSCAALPETLLEAELFGHEK
                     GAFTGALRQRHGRFELAHQGTLFLDEIGEISPLVQVKLLRVLQERQFERVGGNDTIEV
                     DVRLVCATQKDLRKEVEQGRFREDLYYRLNVVPVQLPPLRERREDIFMIAQHFLKTMS
                     GRNHQEPKALSRQAREVLLQYSFPGNVRELENTIERALALAQHTQEIQVWDLCGQSRC
                     PYAGGEPQKGCGLCGDQEGRRGVKNGAMETLAEARENFERGHILEILNRTGWSRMTAS
                     RVLGLSRKGLWEKCKRYGIVRACDDTNSEKDDRDDD"
     gene            complement(26786..27880)
                     /locus_tag="PKM72_03490"
     CDS             complement(26786..27880)
                     /locus_tag="PKM72_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNP59876.1"
                     /translation="MTQPTSPDTLSSNPGFIHNPDFQPTPLIDSLAYWKSGVRELKSF
                     RGHTQEIWSVSFSPDGLTLASGGNDRFIRIWDIETGRLLRSLRGHTLTVREVKHSPDG
                     QMIASASEDRTIRLWNPQTGESLRLLFTRYDHGVTSISFSPDGLMLARAGQNKDIKIW
                     EVTTGTELMTLLGKDQYDHNWSVCTAFSPDGIHLVAGTDVGKIKLYEVLPSGEERIVH
                     NGHWRDDADDESTESRGFYVDDQGGFQKPMEYWIGALTFTPDGGTMISGSRDCTIKFW
                     DMPTMEERRTLKGHSAWVRNLLVTQDGQVLISASDDGTVKMWDIQTGRQFRTIRSHKG
                     PVRGITLSSDGKYLATAGNDRIITLWEGGE"
     gene            28136..29134
                     /locus_tag="PKM72_03495"
     CDS             28136..29134
                     /locus_tag="PKM72_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59877.1"
                     /translation="MEIRFQPALLQEVIDSFAEKTEREGDPTYFNEFHEFADPIYEKF
                     SLDDRDPEFKRLYQHLFAKWGFAEILRDAFDDFPVLRDKTGIVLVRGVLKEDQEGVDV
                     LRKWGVVEEKLSRKFEEAGKKGVGIKLIPRRFYDPAITRYLRHELTHISDMLDEAFGY
                     DPDTKVGLNPGEEHLILNRYRVLWNLHVDSRIARTGKEPMFSKEYRFREFRSWYRKIL
                     PGQVESVFEGIWQADYLTHAELVEMASDTIRVIERAIEVEDSEVPDVPTKPMLLPGFP
                     CPLCRFPTYTWVENMDETLEGFVLDYIRENHPGWDVEYGACDRCVEVYKLRASGVV"
     gene            29251..29805
                     /locus_tag="PKM72_03500"
     CDS             29251..29805
                     /locus_tag="PKM72_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNP59878.1"
                     /translation="MFLRQSAISIGATVHEFVKHRDAPSPIKKEQPVPEKTGWLRPPG
                     AVEEKLFLERCTGCADCVEVCPHDAIQVLSSNGTPVIYPGESPCKLCDDLPCIGACTT
                     EALLPVEDSFQVNMGVAKVWAKMCTAGTGCNACVSKCPTDALSMDFSSYHIRVDDSVC
                     VGCGICQYICGTVNDRTAIRIVPR"
BASE COUNT         7336 a         6060 c         8112 g         8305 t
ORIGIN      
        1 gtgggatgat tgccatgctc gccttcttta tcagctatta tttgtttcgc agtctgggtg
       61 tcctggcttt tgagggaaag gaaggctttg gcggacctca atggttatat gaccaggtgt
      121 ttgtccctgt cttgaccctt catattctcc tggtcgtgat cggcctcgtg atggccattt
      181 atatgatggt gttggggttc cggtcacaga ctttcgtgga agggaagcgg gttttgaagg
      241 atgcgatatt aaagacttcc gggagacgta ttgcgatgat cctgggaggg gtgaccacca
      301 ttgtcctcct atttttcctg tcacgggtca tgacctctgg attttccatg ggaaaattca
      361 gtgtgtatct tggtcttatt cttctcattg cattggtgtt tggcattgaa atatctatcc
      421 agcggatttg gccaaatggg gccagacggc atcgagtgct tggacgattc accatggtga
      481 tttactgtgt cttgttcgta acgggaacgt tcacctacac gatgctctac atcctctatc
      541 caggaaaaat tgggtaggct gttcatgcaa tgcggttgtg attatttctt gcggatcatg
      601 gatattgaag agttgccctc cgattctgtg tttgccaaat ttcattgcga tcggtgtgaa
      661 ttttctctcg gggccgaagg aagtgccaag gaattggatt ctatggtccc tcacacttta
      721 tggtcagatg aggctttata tcagttgagc cggcttcctc cctatttact tccccttgtt
      781 cgggaagagg tggaggagtt tgcctctacc agggggcaaa gggttgtgac ggtttcccga
      841 gttgggtcgg cgcggaataa ggggatggtg gagtggagcc cggacgcaga gcgccgtatc
      901 aataatgttc cgtcaggaat tcgggcaatg gcgagaattg aattggagcg tacagctttg
      961 gatcgcggga tgcctgcggt cacggttgca ctcatggaag aggtgaaagc ccgttatttt
     1021 ggtatggcgg cagggcgcgg atgaaacgcc ttgtgagcga attagcgaaa acgattaatc
     1081 tctggaaatc tcttcaaagc gagtaacgat atgttgcatc gactttccct tcgtgtcctt
     1141 ccgcaattga atgccttagt aacggatcaa cccgttcgca agcggaaacg tttggtgatg
     1201 ctggctcccg gcttagtggc ctttgggatc tatcaccttc tcaagcctgt cttgccgctt
     1261 gccgatccgt tggtgcttct ggcattcacc ggctgtattt gtatgcttac cgccatctgg
     1321 tcctatcgac agggacgtgg agtctctctc atggaatcct ggcgcgaaga cggggtaaag
     1381 cagtgggtct ggttggtggg atggattggt ctggtctatg ggattcaatt atcccttttg
     1441 gttttggcga ttcttcaagt ttttgtggcc tatgattttt tgctccatcc ggaaggtcca
     1501 gccatgatgg caaccatcat ttcatgcacg tctgtcaccc gggatgcctt tgaaatcggg
     1561 catgtgcaac ggatgaggca acaaggggtt gtcgtcccaa cctttcctga cggagaggcg
     1621 ttacgaaaat ggattccgca ggaaatagga agcattgtga aatggggagc gttggcaacc
     1681 atgataggga tagccggttc gtggctcctg ttcactgtgg gaccgattga actgcaaccc
     1741 atccttcaat cattgcttgt tcccgttatg atcgcctcca tcggaatgaa gacgtttttc
     1801 agcggggaat cgttatatgc tcaaaataca cagggaagta ggccacgaac ctggctgtca
     1861 tccctttggt tttggatgtg gccaaatctg accttttcag cgacgtattt tctggttctc
     1921 atggggatgg ccgcctttgt cttgcgggtg gagcagattt cccaagtagg atttatgggg
     1981 atagccgcct gtaccgccat ggtgatggcg ctgtataccc tctttctggg ttggcgcaaa
     2041 tcctatgaag aacagcttgt cggcattcct gaatccatac aacggtgtcc ctttgtgatg
     2101 gggatactcc aaaattctaa atcctcagaa cctcttcctt ccctcgctga tgccaaagtc
     2161 gggcatggca atttggtgaa ttaaattgaa aaatcttttc aaaccattat tgagtgtgct
     2221 gttcatcttt ctcatgcagt ccggaattgc gttgggtatg gatgcttatt ccttaccggc
     2281 aactgatggg caggcaagcc aggatatttt ctacaaaact cccggaacga ttgagggtcc
     2341 atctgtcgcg gagacccacc aggattattc agcttatcct ctcgtggata accggttggt
     2401 ggtctggttt gtgactcagc aacacaccta ctttggggga tttgtcttat caattcccct
     2461 attttgtcta ttattggaat tcataggaat tactcgaaag cagccggcat cccaacagaa
     2521 gttcgatgga ctggctcgtg atattttgcg cgtggccctg ttgtccttgt ccataaccgc
     2581 tttattaggc tgtctcatgc tcataacctt tgtgactctt taccctgggt ttatgagtta
     2641 catgggatcg acgtttaagc ccgtcatgcc ggtgtatgcc acggtgtttg tgagtgaagc
     2701 attcctgttg gccctctatt attacacctg ggatttcctc aagactccgg cggcaaagtg
     2761 ggtccatatg actcttggag ttctttcaaa cgcaaccgga gtggttttac tgttgttagc
     2821 caattcatgg gcttcgttta tgatggcacc agccggagtt gatgccgaag ggcacttttt
     2881 aggaaatgtg tggcatcttt tgcattctcc attatggaat ccactgaaca cgcatcgctt
     2941 tttggcggat attatgtcag gtggcgccgt ggtcgtcgcc tatgcgacct accggtattt
     3001 catggcaaaa acccctgagg atcgggcgta ttatgattgg gttgggcatg tctttattgt
     3061 ggttgtggtc tgtgcgctcc tgcccatgcc acttgccggc tactggttga tgcgctctgt
     3121 gtttgagttt cggcaaacta tgggcatggc catgatgggg ggcatgttgt cgtggctgtt
     3181 tgtgcttcaa gccattatgg ttggtgtctt gtttttggga attaattatt acatctggca
     3241 atcattggcc cgccttcagg gtggtgagcg ctttcatcca cattttaagg ccattctctt
     3301 tacattgatg gtgtgttttc tcgtgtggtt taccccgcac accattgcga tgagtgggag
     3361 tgagatgaaa gccatgggcg ggcaacagca tccggtgctt gggaattatg gggtcatgtc
     3421 cgcaaagaat ggtgcggtta atgtcatgat ctgtttaacc gcactgagtt acattctgta
     3481 tcggcgagcc aattatgtga tgacagtacc gtgggcatcc aagggaaata tttttcttgt
     3541 ggggttgttc ctcatgggga ttgccaatat tgtgtggttg gctatttacg gattttatat
     3601 tccggcaaat gtccgcgtgg gcctttctta ccctcaaggg atgacgaccg cgactctagt
     3661 ggttggtggg ttattgttga atcggttgtt attgcgtggc gctcacatga acgggcctgt
     3721 gcattgggga cgaattaccc cgcgtgggat tgtggcattg tttgttgtgg ctgccgcctt
     3781 tacctgggta atggggttga tggggtatat acggtccgtt ggccgattgg agtggcatat
     3841 ttccgaaata atgccggatc aatcctcgtg ggcatttacc ccttctctgg gatttgcagc
     3901 aaaaatggtc accctcaata tgattgtttt ttggtcttca gtctttttcg tgttttggct
     3961 tagccgacgg gatcaacaag tggtagagcg gcgaacggct gaatttgcaa ggccttctcc
     4021 ggtgattcag gttatttcag aagaggaatc cgcataatga aagtttatca tgggcaatac
     4081 aataatttag gaaatgtgaa gcgagtccgt gcaaggtctt cttttttggc ctgtttaatc
     4141 gtgagtattt tagtatatgg aagtggtgcc gcatgggccc aagaggtttc gaacacaggg
     4201 tcttccagta gttcgatggt gttggaggat tttcagcatc ctgatgctaa aggatttccc
     4261 gtgggatggg aagcgcagcg gagtacggtc actgcccatg aaacctatac gattcaggaa
     4321 gaggatggga cttttttcct ttcggcaaaa gacgccaatc aacgagttta taccaagcac
     4381 atgacgtggg acccgaagca gcaccctatt ctgacctggc gttggcggat tcagacggtt
     4441 cccgataacg ccgatttcct ggccgcaatc tacccgtcat tggatgttga cttgatgttt
     4501 attcccgtga atacgaaata tgtgtggagt gcaacgcttc cggtagggtc agtcaaagag
     4561 ggtggcatgt ttagctctac ggaaattgtc attcgtagtg ggactgaatc ccttgggacg
     4621 tgggttgagg agcgggttaa tgtatatgaa gattttctga ggattcatga tcatgagccg
     4681 gctccacatg cctggggtat ttctttgtta ggagggccgg gagtggaagt ggactttggg
     4741 tccattcagg ttcaggctga atcatccaaa ggtgatcttg tgcgtgaatg aagagtgaaa
     4801 agtgaagata actccagcta aaatttttga tgtcctcctt ggagtcctgg gattagggac
     4861 agtgggcctc ttgatcgggg tcttcatggg ggatgggtgg cttcctgttg cgttagcatt
     4921 gggagccatt cttggggccg gagtgggctt ggtaggcgga cgaggatttt ttttgagtat
     4981 ttttatcgga accattttag gtggtttgct gacattagga gtgagtggaa ccgaggctgt
     5041 gacggttggc gcagcctcgg gtgcggcaat gggtggattt ttgggaattt ggatttcgat
     5101 gcttatagag acgtggcgac aacggaatca aaccgttcct gagtgcgaca agcatcctaa
     5161 tcatttacaa ccgtaatcaa tctgaatcat cgaggtgagc gtgtcaaagc gagaggcaag
     5221 gtagtcatgg aaaatcagcg ggtactcatc ggctcaattt tatttgtatt cggctcattt
     5281 atcatgatga tcgtgatgtt gatttatatg acttataaag ggaaggctga tttggcggaa
     5341 ttgtcggccg gccaaccggc caacgtcagg gtcttgcagc caatgcccac acaagatttt
     5401 tccatgtata aaacgtttgt cggggatgat ggccgggaaa tggtcgaaat tcctgaaggg
     5461 ccgtttacca tgggcattga ggatggggat cccgatgaag ggccatcgca tccggtgtat
     5521 ctccaaacgt tttatatcga ccttaaagaa gtcacacaag cagattatga tcggtatatc
     5581 aagatgacga aacgggacaa acctaaggtg ccggtctttg aagatgatgt ctccaagctg
     5641 gtcggtctcg attatccggt ggtggctgtg acctggaacg atgcctttgg gtattgtcag
     5701 tgggctggga agcggttgcc gaccgaagcg gagtgggaaa aggcggccag gggagaaggg
     5761 aagcggcgtt atccttgggg ggaaaaattc gaatatcagt ttgccaatgt tgatgggaag
     5821 gaagatggct ttcagtattt agctcctgtg ggatcctttg aggtagggag aagccctttc
     5881 ggactctatg atgccaccgg caatgtagcc gagtgggtca tggatagtta tgctgctgac
     5941 tattatcaac aggccccata tcgggatccc ccaggaccaa aagtcgaaga tgagaataaa
     6001 gtcattcgtg ggggctcttg gcgagaatct cgtatcggcg ccagggttac caagcgtttt
     6061 gcagctaaaa tgtggaggaa cgatgccagt gttggatttc ggtgcgcaaa agatcctccg
     6121 gccactgaga ctcctcaatc ctcctaagcg gtaagcaggc tcctcgccgg cgtgaataat
     6181 cgcccatggt ttctcgagat aggacacatt gacggataag gaatagggag acatggacgg
     6241 tcgatttaaa ttaatttttc tgattgcggt cctgtttatg actggcctgc cattattagg
     6301 gattttgaaa gggaccgaca ctcccccaag tctcccggac ccaactcccc aatcaatgcc
     6361 ttcttcagca ttggagccgt ccacagaatc gatggttcct gatcctgaca tagggaatga
     6421 gatggttgag atacctgcag gggaattcat tcttggcagt aatgaaggcg gatttaatga
     6481 aaagcctgcc cacgtcgcta atttagggac gtactggatt gatcgatatg aagtcaccta
     6541 taaacggtat atggaatttg tggaagctac aggccatcgg caacctggtc cgccatctcg
     6601 ttatgccgca aagctcggac tgttaagagg gccttatcag ccgattacct atgtgtcatg
     6661 gagcgatgcg aatgactatt gtcagtggaa aggcaagcgt ctccctactg aacaggaatg
     6721 ggaaaaagct atgcgaggga cagatggacg cacctggccg tggggtgatg ggttgaccgg
     6781 acatcctgcc aattttgcag gggagggcga tgggtttgaa gtatcggctc cagtaggttc
     6841 gtttcccctt gatcaaagtg tattcggggt ctttgacggg gcaggaaatg ttatggaatg
     6901 gacggataat tggtacgtgg aagatctata tctacagaaa gattcagttt cgcaagaaaa
     6961 taatcggcca acctcgactt acaagacaat gagaggaagt gggtatacga gccgaggcgt
     7021 agatctccga atcaccaata ggagttttat ggttcctgat tttcgagatg agacaattgg
     7081 tttcagatgt gcacgttcaa attaatgaca ataaaggtga cgaaattctg ttgaggaaaa
     7141 tcaaaaaaat taaagaaaat caaaatagta gaggatgaga aagacggcaa gcagaatatt
     7201 gacaaccatt ccggccaaaa ctataatgtc gaacactttt gcatttgtac gcatgtgcaa
     7261 tccctctttt tacgtgatca ataaatgttt gaatagcacg aaaaattata ttatgtcaat
     7321 gaagtttatt agggaaaatc atttaagatt cagtttcgaa ttaagcacag ggaggaattg
     7381 aaatgggtga ttcgacggct gtcataagag agtcaacgga gttagagact aaaattggaa
     7441 aagcgatttt ttatgtaacc tgctcggtaa gcctttggtt tttctattgg ttttcaggca
     7501 ttcagtgccc ctgctaaaaa agcatgattt ccaataagac aatcaattat ttaatactct
     7561 tagacgcaag gaggggcgca tgcagacagc tctgtatgta atagtttcat tggttatttg
     7621 ttttgtgtat tttcaggcac tggattatgt actgatggac gctcaggggt tggactattt
     7681 ctacatgttc agatagtcgt aagaatggaa atatttaaga ttcacattca tgacgaattt
     7741 taggtaaatt tgatgggttt aaacacaaca cgttttcaca aggaggtagg tcaatgggtc
     7801 gcctgttcaa tcaaatgacc cgggggaaga aaaagttgtt cgccgtttcg gccctgatgg
     7861 taatgggcgg tctgcttttg ctgccaattt tcctgtcagt ctcagctgtg gcgagtggtg
     7921 gcggggcacc agcaggtggt cctccaccag atgtcgttgc cgcacaaaag gcgggagagg
     7981 aaggcgaggc ggaaaaggtg gagatgggcc gtgacattta ttacaagacg gaaggtccag
     8041 ccattggaga gggcgcaccg gtaacggccg acaacgaaac tttttatcct cggtataact
     8101 tcgagagtcg tgtcctcctt tgggtagcca accagcagca tctttattac ggtagctttg
     8161 tgttggcggt tccaatcttt tgtatgtgta tcgaatttgc cggtatggtg agcaaagata
     8221 aggctatggc caagaagtat gaccaattgg cttatgattt tatcaagatc agtctgacgg
     8281 cctattcttt aactgcgatc ctgggtggga tcctgatctt taccttcctg actctttatc
     8341 ctgccttttt tggatactta tctagtattt tccgtccggt catgcacatt tatgcattaa
     8401 cattcgtggc ggaaagtgca accctctaca tttattacta cggatgggac aagatgaggg
     8461 aaggggtcct gaagtgggtt catctcagca tgtccgtgat tctgaatgtg attggaacgg
     8521 tgctgatgtt cctcgctaat tcatggattg cgtttatgat gtccccggct ggagttgatg
     8581 agcaggggag atatcttgga aatatttggc atgtaattca tacggccctt tggaatcctt
     8641 tgaatgttca taggattctt gggaacatgg catttggtgg tggtgtggta gctgcctatg
     8701 ctgcctatcg cttcctttca tcaaaaacgg atgaagagcg tgctcattat gactggatgg
     8761 gctatatcgc aatgagttta ggtgtggcgt ttttaattcc gctacccttt gccggatatt
     8821 ggttgatgcg ggaggtgtat gcctaccgtc agcagatggg aattacctta atgggtgggt
     8881 tgctggcttg gttatttatt attcaagcca ccatgattgg aattctattc ctcaccacca
     8941 attattacct gtggcaagct cttggccgta tgacgggtgg agagcgtttc caaaggtata
     9001 ttaaatatct cgtgtttatc ttagtggttg ggcttctggt gtttattacg ccgcacacga
     9061 tcgtgatgtc tcccgcggaa ttgaaagcaa tgggcgggca gcagcatccg gtcctcggaa
     9121 attatggtgt catgtctgcc aaaaatggtg gaattaacgc cattatcatg acaaccattc
     9181 tgagttttat ttggtaccag cgagggaaca gggtccccac agttagctgg gctaagtttg
     9241 gtaatatttt tatgggctgt ttttttgtca tagcacagct aaataatgtc tggttggctt
     9301 gttatgggta ctttatacct gcaaacgtac gaatcggttt gtctgtgcct caggtggcgg
     9361 gaaccttgtc ttgtctgctc ttaatgaccc cgctcaacct ggcgatgttg aaaaatggaa
     9421 gacaattggg gccgatcaga tggggccaaa ttccaccacg ttcacaatat gcgatcatca
     9481 tgttggccac agcattcacg tggatgatgg gattaatggg gtatatccgt tcttcggtgc
     9541 gattattctg gcacgtgaat gaagtaatgc gggataattc tccatgggcc tacacccata
     9601 caattggttt tgcggcaaac gttatttcgt ttaatgtgct gtttttctgg atcagtataa
     9661 tgtttgtctt ttggcttggc acactaggtg ctaaaaaaat accggttcct tcgcctgcag
     9721 ggcagccagt tccatctcct caatctgcaa ctggtcatta acgaaaaata caattacaaa
     9781 caatcgtggg gctgcattag gcagccccca agttggagga ttgcactgtg gttgagttat
     9841 tgggacgggc gataggaatg ggttggccag tattactgat gctggttggg ctattgctgt
     9901 atttccaggc aactatttcg gatcctgaaa aaaagaaaag ggcaagtttt cagaccataa
     9961 ttggaatttt ttgtgctttt ttagcgttta tcgctatttc caattatacc cataattttg
    10021 aaggggaaag tcggttgctg ccggtttccc ttgtcatgat cacaatcatg tcttttatta
    10081 tgggtctcta ttttccgaat atcagtgccc taatgaaaat tggagggttc atgttttttg
    10141 tggcggccgc gctttccggg tacggaaatt ggctgcctca ggtcgagggc ggatttcctc
    10201 caccggtcgt gaagttagac ttccaaagta tgtcaccgca acaacttggt gatgaagggg
    10261 aaaaaattat atttggtggg attgggcaaa gtaaaaccca aggagccata gggaaaggcc
    10321 agtgcccgtt gtgtcatggg ttccaacagg gatttttaag tgaacgggca cctaatttat
    10381 ttggaattcc tgaaagatct gaagaaagac ttaaagagcc aaattaccat atgaataatg
    10441 ccgctgctcg aactactgaa caaaaggaag cttttgaagg atcggggacg gctacaaatg
    10501 ctcaggaata cattgctgag tcacatgcat gcccgagttg ttttgtagta acgggatttg
    10561 gggtcaaagg atcaaatgat accgtaagtc ccatgcctaa gattcataaa ccaccgatat
    10621 ctttaacaat cggcgaaatg gcagcagtgg atacctggat gtatacgagg gaaggaaaag
    10681 aagctcctgc ttatgatgtg attgctacct catatgaaaa gttcattcca gaagccgatc
    10741 gcccctctgc tggtggagaa gaagaagctg gtggtggtgg tgggaatctt ttagctgacg
    10801 ggagcgagcc ttacgacaag ctctttatga aagccggttg tcctgcctgt cataccattc
    10861 ccgggattga aggagccacg ggaaaagttg gtcctcttct catggaggga tcaaatgctc
    10921 ccaatcggat taaagatccc aactataagg gtaaagcaaa atctccaaag gagtacatta
    10981 ctgaatcgat attagaccca agtgcctacg tggtgaaaga ttttcctgac gatcaaatgc
    11041 caaaggactt tggtgtaagg ctcacaggcg gtgcacttag taagatggtc gattacttgg
    11101 cgcaattaaa agaaggccaa cctttacctc caaaagaata agaaatatta atttagggtt
    11161 taacccttta taaggagtaa ggaagaatga cgtggcttaa attagttgaa ggctacatgc
    11221 cgatgcagat gatttccgag ttggcgatct gcatccttgt gttttctatt attaattact
    11281 ctctcaagag agcaggcatg ggattgccaa agttttgggc aggaatcttt gtctggtgtt
    11341 tcgtgaattt gttttatttg aaatatcgaa tttacccacc aataccattt agtgtgcggg
    11401 ctatttatgg caccgtggcc gcttgcggta tttttatgtg ggtttcgggg tcacagcagg
    11461 aatgggaaga attcaagcgc ccaattatca atgtgttgga cgcaaaaacg ggtttgacca
    11521 agttcgttcg tacggcccta ttgattttat taccaattgg actctgggga tttgcctaca
    11581 actccttcct gccaagtttt gatgaaccaa ttgagctaag aactgtgcat ccggccccac
    11641 cggccacaac taaagtacat gggaaaacct atgtattgca aaccgcagct aatccttttc
    11701 gtatcaatac tgaagggaaa tacgatcagg catacagcaa cgctcatatc gtcagccagg
    11761 atatggggcg attaatgaag gatgtgaaaa atccggaaga taacccctgg gatcccaatg
    11821 cgaaaggcta catcaaacat gttcgtgagg ggggggagat cttttttcaa aattgccatt
    11881 tttgccatgg ggataattta aatggccgtg gtctttgggc attcgccttc aatccaatac
    11941 ctgccaattt tacggatgca ggaaccatcg ctcagctaca ggaaaccttt gttttctgga
    12001 gggttgctaa aggtggaatt ggtcttcctg gtgaggggtt cccatgggca tcagttatgc
    12061 caccgtggga acagcattta accgttgatg aaatatggaa agtcatcatg tttgaatact
    12121 ggcacaccgg gtattacccg cggacttggg aataaggagg acgcggacat gaatggtaat
    12181 aaatcccaaa atggactttt tgaaaagagg atggtcatga acgaaacgac gacaccggcc
    12241 gaatatggaa ggaggggttt aggagcctgg cttctgagtt gcggtctcct gattggtggc
    12301 ctaggcctag ccaccacctc ccacgcggaa atggcggaag gatttgccga agggagtcgc
    12361 cccgccccgc catctgcgga acaggttgaa gcagggaagc gagtgtattt caccaaatgt
    12421 gtatggtgtc atggggttga gggggctggt gacggacccg gagccgatcg attatggccg
    12481 cgtccacgga attttaatgc agggacattt aaaatccgac ataccgcgag cggtgaattg
    12541 cccctaatag acgtggattt atttcaaacc gttacccatg gattgcctgg ttcggccatg
    12601 ccgtcatggg agggtatttt gaccgaagat caacgcagag atgtattggc ttttgtgaca
    12661 actgagttag tcaaggatcg tagttggcag gatacggaat ttgaagaatt ccatgttttg
    12721 caattggata aaattcaacc ggttgctccc tcggctgaat caatcaaacg cggttcggag
    12781 ttagtaaaag aaatgaaatg tatcgaatgc catggggtag aaggtcgggg agatgggaat
    12841 gcctttaatc tcaaagatga ctggggcttt tccattcaac ctgcggattg gcataaatgc
    12901 tggaatttca gaggcagccg acaagatgcc tataatgtaa aaaacatttt ccggacattt
    12961 tccacagggg taagtggaac gccgatgcca tcttttgcag ataatactac tgtagaggac
    13021 aggtggcata ttgccaatta cgtcaatcat ctttgtgaac gtgatgttga tgtggatatt
    13081 gccggcggta atgtgacgga tgaaattgct gcggctttac tagcggcaaa gcccagggga
    13141 attgatcctc ttacggataa acccaaagtt gatttcgtgg ttccttctaa gtttgtcgaa
    13201 ggagagttgc cggctgacga gcatgatgaa cggtggaaac tggttgaccg tcgcattgta
    13261 gccatgggag ggcagatcac ccataaacca agaaactttg tgacaagaat tgacgatgtg
    13321 tgggttcaat ccttgtacaa tgaaacccat atctcattta tgttccgttg ggatgaccgg
    13381 accaagagtg tgcagcaaga tagcgttgac tgggaaccct atgaggtaaa tcttggagat
    13441 tacggtatag aagaacaggc accaggagga tcaaaatttg ccgatgatcc tgaacaccct
    13501 gaatcgattg ccgcgaaaca aacggcctat caagtgttta atgacgggct ggcttttcag
    13561 ttcccaataa aatggcagga attgcctgcg ccaagaaagc ctagatattt ctggggagat
    13621 gagggatttc ccgtggacat cgccaaatgg acggctgatg gagaattgaa agcgtaccaa
    13681 ggtgaaggtt gggatgttga tttcgaagat cgtgacgatt tcaccgaaga actgaaaact
    13741 gtgaaagctg aatggaaaga tggacgatgg acggttatca taacgcgccc actcaagggt
    13801 gactatgagg aagatgctta cattgaattg ggaaaatata ttcctattaa tttctttgtt
    13861 tgggatggtc acaatgggga tgtcgggcga aagatggcag tctccgcttt ctattatctc
    13921 gtgttagagc cacccattga aaaagaaaca tatatttacc caacactggc cgccattggg
    13981 ttggtccttg ttgaagggtg gattttgacc cgacgggcta atcgtcgtaa gggtaaagtc
    14041 taaggaaaat gtgaacttcg gaaatatccg aaaaaatgaa gggggtgggc aaccaccccc
    14101 tttttttatg accaatatta aaaaatttac aaacatttcc ggtgtagtct tagcaggcgg
    14161 gaaaagccgg aggatgggga aagataagag aatcctcgaa tgggaggggg ttaaatttct
    14221 cgataaagtt tgcctcaccc tcgtagaatt atttgatgag atcattgtgg tgactgccat
    14281 tgaagattac ccttgcggac acctccctgt tagatcagtg accgacgcca ttcctcatac
    14341 aggttcctta gggggcatgt ttaccgggat caaggaggcc tctcattctt ctgtatttgt
    14401 agtggcctgt gatatgccat ttttaaatcc atcggttatt tcaagactgt gcgctttacc
    14461 tgataatgat gtggtgatgg ccaatctttc aactggatat caacctcttc atgcacgata
    14521 ttccaaacgt tgctccctca taatggagaa gatgattcag gacgggaatc ttcgaattca
    14581 tagcctgatt caggactcat ctctctcggt ccaaatcgtt gaggaatctt tattcgatga
    14641 tatcgatcct catgggtatt cttttttgaa tattaatacc cccgttgatt atgaattcgc
    14701 tagaaaagct gcttctcatc tgaaataata gtatgcctcc cgcccgccac aatttattga
    14761 ttattcatcc tggagcatta ggggatggat tattagctct gcctgctctt cggatattgc
    14821 agattagatt tcctgaccat cgcctaattt ggtttggtca taaggaattg ggtgaggtac
    14881 ttgtgcatac gcaggaggtt catcaggcct attcttttga tagactggaa tttttggcat
    14941 atcgtgtgga aaatgactcc cttcaagaga attttctgtc gattctccgt cgttgcgata
    15001 gagccatatg ttggttagag gacacagacg gaatttggct aagttggttg aagactgcgg
    15061 gaattccaaa ctgtattctt cggtcccctc gtgacccgac attgataaat aaacatatgg
    15121 ttgatcggta tgttgaaata cttcaacctt gggctaggac gacagaggtc cgtagtgaca
    15181 taggagcaca tgagaggcct gcacggcatc ttgttttcca caaatcactc tcgttaagag
    15241 tccattctcc catcaaggag ccacttattg ttcttcatgt tggaagcggt tgtcgatata
    15301 aatgtgcctc tccgatgttt tgggcgagta ttgtaaatgg cctgatgagg acgaatccga
    15361 aatggaagat ttgtcttgta ggaggacccg cagatattga ctctcttcga aatgtgcaag
    15421 ggttactgac tgacattgag tatgccactc ttaccggaat ggatctgctt caggtcgcaa
    15481 aatatttaca atatgcccaa atatttattg ggcatgattc tggactttcg catttcgctg
    15541 caagctttgg catttcttcc gtattgttgt ttgggccaac cgatccggca aaatgggcgc
    15601 cgcagggagc tcatgtaacc attatacgaa aatcctgtca ttgcattggt aagacgtcca
    15661 ttgctggttg tatggataga caatgtcttt ctgtttctcc acttgaagtc ctcggaaagg
    15721 ttgaagaggt attatgtgga gtgaagacag ccgtaaatta cccagccttt gagtgcgttg
    15781 atgaagactt aactgtgcct tgccttggtt agaatgctgt gctacattcg ccgcggccat
    15841 tgaaaaaccc atatattctg cggagatagg atgtttcagc gtggagaaaa tcctccaaaa
    15901 ccaagtcatt gaggcaattg cggttatcct tagcacggcc aaggaatccg gcgagttaat
    15961 ggtggatacg attcctacca tcgttgtgga acctcctaaa cggccggaat ggggagactt
    16021 ttcttctagt gtcgcaatga cattggcctc acaagctcga caaccacctt tgaatgtagc
    16081 cgaactgttg gcagcaggcc ttcgtgcgca gttttcagat ctattcgtcc acgttaacgt
    16141 tgtccctcca ggatttttga atttgaccct ccatccccac cggtggattc aagtcctacg
    16201 gatgattcag gacaagggat cgttatatgg aaccagcgac attggaaaag ggaaacgcat
    16261 cctcctggag tttgtgagtg cgaatccgac aggcccgttg catgtcggcc atggacgagg
    16321 tgctgcattg gggcaagcca ttgccagctt attgaaatct accggatttt cagttttccg
    16381 tgaatattat attaacgatg ccggtcgcca attacagttg ttgggccgtt ccgtccatgc
    16441 ccgttatcga gagcattggg gcaaaccgtc atcgtttccc gaggatggct atcatggaga
    16501 ctatataaaa attgtggctg aatcggtggc gaagactcat gggcaaacct tactggatga
    16561 atcatcggaa aaggcggaga cactctgtgc gcaattggcc agtcagatgc ttttggatcg
    16621 aatcaaagaa gatttggcca catttggtgt tgaatttgaa acctggttta gcgagacatc
    16681 cctgcataag actggtctta ttcaacagtc gttggacgaa cttcgacaga aaaggctgct
    16741 catagaagag gagggagcct ggtggtttcg ttcatctcag tttggagatg aaaaagatcg
    16801 tgtggctcaa aaacaggatg ggagctatac ctatttagcg gccgatatag cctaccatcg
    16861 acaaaaatta gaacgtgggt ttgatacctt aataaatatt tggggtgctg atcatcatgg
    16921 ctatattcca cggatggaag ccacagttca agcgtttggc tttgccaagg agactttgcg
    16981 gatcgttttg gtgcaaatgg tgagtctgcg acgtggtggg caaaaaattg agatgtcgaa
    17041 gcgagctggt gaatttgtga ccctacgcga ggtgattgat gaggtgggtc cagatgccgc
    17101 taagttcttt ttcctgatga gacgagccga tacgcatttg gattttgact tggatttagc
    17161 caaacaacag tcttcggata atcccgtcta ttatgttcag tatgcgcatg ctcgattggc
    17221 cagtctcttt cgagttgccc aggaacgcca agtacgcctt ccggtcatcc aggacgttaa
    17281 gcaggcatta ttgattcagg atgaagagtt agggcttatt aaaacattgg cacagtatcc
    17341 tttggtggtc gaaagcagtg ctctcgcgtt agagcctcat cgggtgacgt tttatctcca
    17401 agagcttgct gcgcaattgc atgcctacta caacaaaaat cgggtccttc cctcattgga
    17461 tcctgagagg gacactgggg gtaggggaga aaatattaaa ttcgagacaa gtgggaatac
    17521 tggaacgttg aatcaggagc tcaggtacga aagtatttcg ccggatttga ccgctgctcg
    17581 cttagccctg cttcggcaag tccagacggt tcttggtaat gggctatctc tcctgggaat
    17641 atccgcaccg gagaaaatgt aacgtgcgtg aaggtcaagc attcgcgaag ataatgcctc
    17701 aagagccatc catctcacag tcaggtccaa atcgtttcca tattcagcat actgtgcctt
    17761 cgggtcgtgc gagggttgga cgattattta ccgcccatgg agagatcgag actccggcat
    17821 ttatgccggt tggctcacag gggacggtca agggactgga cccgaaggaa gtccgcgaat
    17881 gcgggtttca tatgattttg gcgaatgcct atcatctctt tttgcgacct ggccatgaac
    17941 tcattgaatc tctgggaggg cttcatgcct ttatgcaatg gtccggagcg attttgactg
    18001 atagtggtgg gtaccagatg gtgagcctgg cagatctttg taaggtgacg gaggagggag
    18061 tcggctttcg ttcccatatc gacggtgcgt ggcatttact ctcaccggaa aaaagtatgg
    18121 ccatccaagt cgcacttgga tccgatatta tgatggttct tgaccattgt ccgacctttc
    18181 cttgtaccga gcagcaagcg cgtgaagccg tacagcgaac gaccaggtgg gccaagcgat
    18241 gtgcggtggt gccgagaaaa gagcaccaat ggttatttgg aattgttcag ggtggagttt
    18301 ttacccagtt gagagaagag tcgactcagg agttggttaa tctgaacatg gatgggtatg
    18361 ccttgggtgg attatccttg ggggaagaaa agccagccat gttgaccatg atcgagacgg
    18421 taattgatca gcttccccct agccacccgc gttacttaat gggagttggg cttcctgaag
    18481 atattgtgga aggggtaggc cgaggattgg atttatttga ttgcgtcatc ccgacccggc
    18541 atggaagaac tggctggctg tttacctcta cagggaaggt gttgattaaa caggcccgct
    18601 actctcgtga tccaaatccc atagatgccg aatgtggttg tcctgtatgc caacggtttt
    18661 ccagggcata tttgcgacac ctgtttttat cgcatgaaat gttgggtgta cgtctgaata
    18721 cggtccataa tctttggtat tatggctcgc tcatgaagga gttacgacaa gctatcagcg
    18781 cgaatcggtt tgacgactac cggagagatt tttaccggcg acgcgaatgt gatccgagtg
    18841 aaacgctcat tcaggatgtg acgacctgat cgaatggatt gaccacagaa acaattacat
    18901 tgaggtactt atgaatttag attcctatgc ctgggcccaa gccggaggag ccggccccga
    18961 atcctcagcc ggcttattat cgctcattcc ttttcttcta atttttgtag tgttttattt
    19021 tttgcttatt ttgccgcagc aacgtcgaca aaaaaaacaa cgggaattgt tggatagcct
    19081 caaaaaaggc gataaggtga ttacgtcttc agggatttgg ggaaccgtga ccaatttgga
    19141 taaggaaacg gctaccctac aagtcgcgga taatacaaaa atccggttgc aacgcgatca
    19201 tgtcgcgagc atccgtacgt cgaatgaatc atgatggatc aaggggtaga tgaatgaaaa
    19261 agcttcgtgg gaggttgttt ctccttttcg tggtcacggt ggtgtccgcc atcctggccc
    19321 ttccttcgtt tccaggtctg ttccagtcgt tacctgatgg ggtcaagagg gtattgagtc
    19381 atcgcggact ctccttgggc ctggatcttc agggaggaat tcacctggtc ctggaagtag
    19441 aagaggagcg tgcggtggag attgccgttg atcgaattcg aaaggctgtc gaggatctgc
    19501 tgaaggataa ggcaatcgag gtggagggtg tgcgtcgtga gggctcgaaa atgattgtca
    19561 tcacccttca acaagaggcg gatggggagg cagtccgaac gcttctcgat gagtctttcc
    19621 cgaactttat gtcgcaaaat tcagcgggaa cgcgcttagt atacgaactc cgagacaccg
    19681 aggaagagcg agttaaaact tcagccatca atcaggcact tgaaaccctc agaaaccgga
    19741 ttgatgaatt tggcgtggcg gaacctctca ttcagcggtt ggggctcaac caaattgcaa
    19801 ttcaattgcc tggggtcaag gaccctcaac gggctaaaga tctcattcaa gaaacggctc
    19861 tcttggaatt taaattattg gaggagtcca aggcggcatt ggatttaccg cctcaagtgg
    19921 aaaaaggcca agaagacacg gtacggaaga cgttggaagg aaaaattcct gaaggggcag
    19981 aaattctatt tgaaacggcg gtttcggaac ccgatgggcg ggcgtatagt attccctatc
    20041 ttgtgaaaaa agatgcggtt ctcattgggg atgtgttgca ggatgcacgc gtgtcaattg
    20101 gtgagttcaa tgagccgatt gtcagtatta cctttgacag caaaggggca cgagaatttg
    20161 atgagctgac ggctgccaat atcggtaagc ggatggctgt ggtcttggat ggaaaggtgt
    20221 attcagcgcc ggtcattcga gatcggatca gcggcggacg agcgatcatt gaaggtacct
    20281 tttccacggc agaagcgaat gatctggctg tggtgctccg ggccggtgca ttaccggcac
    20341 cgttaaaaac cttgcaggat ttaaccgtgg ggccctcact gggacaggat tcaattgaga
    20401 aagggttacg aacaactatt attgccgggt cgttggtcct gatctttatg attgtgtatt
    20461 accgactttc cggcgtcatt gcgaatatgg ccgtttttct taatttaatt tgtttgctgg
    20521 gagccttatc tgggctcaat gccaccttaa ctttgccggg tattgccgga attattttga
    20581 ctattggaat gggagtcgat tccaatgtct tgatatttga gcgaattagg gaggagcttc
    20641 gtcagggccg ccccgttcgt ttagccgtag atagtgggta taataaggcc tttttaacaa
    20701 tcgtcgattc ccatgtgacg accctgatta ccgggttagc actgttttta tttggaaccg
    20761 gacccatcaa ggggtttgcg gtgaccttgt gcttgggcat tgctattaac ttgtttacgg
    20821 cgttggtcgg gacaaaggtg gtatttgatt tcatgaatcg acgaaagttg gatacgctca
    20881 gtatctaatg ggtcatctgg attgagaagt gcttcgtgag cttaattgtc gtggttggtg
    20941 ggagaataga catgcggatt tattgggctt gagcattctt tcatgcgggg cgtcaacgca
    21001 agcatatggg catgagacat tctttgggga ggagcgctga tagattatga tggaaattct
    21061 ggggaaaaca gatattgatt ttatgggaaa gcggaacatt gccttcatgg tatcaggctt
    21121 tctggtttct ctgggattgt tggccgttat cgccattctg gtgggatggg ccaatttggg
    21181 gattgatttt gccgggggga cggccgtcca actgaagttt gataagcccc tcctgattgc
    21241 cgacgccagg aaagccttgg atgcccatgg attgggtgcc gcggaacttc aggagtttcc
    21301 ccaggatcaa aaacttttga tacgcgtgaa aactgaaacc acaattgaag agggcgtcag
    21361 taagaatatc attcaggctt ttcaagctga atttcctgat catgggtttg tggtggactc
    21421 cagtacgtca ataggtccca cgattgggaa gaaactacaa gaagatgcct tgattgccgt
    21481 tatactttcg ctcgcaggaa ttattctcta tgttgccgtt cgttttgagt ttcgttttgg
    21541 cgtggctgcg gccattgcca catttcatga tgtccttgca gtattgggga tttatttttt
    21601 gttgaatacc gaaattacgc tgctggtggt gacggcccta ttgaccctgg caggatattc
    21661 tttgaccgat actgtgattg tgtttgaccg tattcgtgaa aatcttcgac agaggagacg
    21721 tgagacgacg acgacgatga tcaataacgg gattaatcag gtcttaagcc ggacattaat
    21781 tacgacgttg actgttgttc tggtcctggt tccattgacc atttggggag gagaagtgtt
    21841 acacgatttt tctttggcct tattgttggg cgttctcatt ggaacctatt cctcgatttt
    21901 tgtcgcgagt ccgctcctct tactgtggcc tggtgcagag ggaaagctat taggtcgagg
    21961 taaatagact tctctgttac tgtttcttga cagcaaggaa aaaggagcac caaaatgtag
    22021 aaaaatatag gtgttagatg ttctctatgg atttttcatt gttgccaaag tgaaaagctt
    22081 tgggtttccc ctccaactga agggcaagac gatcctggct atggtgctgg ttggtctatt
    22141 gccccttatt ctttcccttc tcctcacata ttttgaagaa aaacgggcac tacgggaagc
    22201 cgccggtatc accatgaagg ggattgccgt ggaagtggct cgtaaagtcg agacccagat
    22261 tattcgtggg atcaatgaag cacaacagtt ggccaccatt ccctttattc gaagtgccgt
    22321 cctaaattcg aaccggagtt atgctgataa aaatccagac gaaattcagg cccttattca
    22381 acagtggcag gaaagttggc ggatccaatc cagtctggat gagtttccgg tatttctgaa
    22441 caaatatgcc acggactacc tgattcaatg gcatgcgatc agaaaatcgg attatctcgc
    22501 catagttgtg gtcgatgcgc aaggagcgct ggtattaacc tcttttccac aagtcaattt
    22561 tttccatgga aacagtctct ggtgggaggc ggtcgttcaa catcatgaaa cccaagcatt
    22621 tgtgagtgat ttgtcatttg atccagagtt tgggactcat gtcctgaatg ttggtgtccc
    22681 tatttgggac gatcttcgtc agcatgtagt gggagccatt agtattcttc tgaggagaga
    22741 ctcgttattt cggtcgattt ctgaagtggc ggccgggaaa acaggtcatg ccatgttaac
    22801 tgcttctgat gggatgccaa ttctctgccc agcattttcc ttggaagagc atgtgatgcc
    22861 ctctaatgtg gtcagtgcgt tccaacaagg tggtgtgggg tggttggttg ctgatccgga
    22921 ttcacatggt atgccgaatg ccatagttgg ctatgctcct ctccacttgg ggatgccact
    22981 tgctccagaa agttttggag gtaagtcctg gcatatgttg accagtcaag actctgccga
    23041 aacgtatgct ccgctggatc aattattaag caaggtcatc ttctatggaa tcgccgtttt
    23101 tgtcatcttg tggggggcgg gggtcgtggt tgctggccgg attgtgaagc ccattcaggt
    23161 cttatcccaa ggagttgagc aatttggaag tgggaatctt tcggaaaaga ttgccatccg
    23221 ttccggtgat gaaatcgaac gtttggctga cacatttaat gccatggcgg agaatcttca
    23281 acattcgttt tttgagttga atcagaaagt tgatgaaatt gggcgtttgg agcagaaata
    23341 tcgtgatctc attgaaaatg ctccggaaat gattcatcaa ttggatcctg cagggcggtt
    23401 cgtgcatgtc aatacgaccg agttgcaaaa gctgggatac tcactagccg aaatgcttga
    23461 gatgtcgctc tgggatattg ttccggcgga acaacaagaa gtggtgcagg cctacgtcca
    23521 agggttagcc ttaggcgggg ctcgctccat cgaaactgtc tttcggacgc aatcgcgtga
    23581 agtgatggat gtggaaattc attccacgac tttgatggat ccaggaaccc atttggtggt
    23641 gtattcgaga ggatttgtga gggatatcac agcacgaaag gctctggagc gtgaggttga
    23701 gcggtatacc aaccaactgg aagggattgt ggctgaacga acccaacaat tgtctgaatc
    23761 agaggcggga tataaggcgc ttttcaattt ggctgccgat tcaatttttg tggtcgattc
    23821 ggaaggccgg atcttggatg tcaatgcccg tgaacgggat attctagggt atgtcccttc
    23881 tgatttagag ggtgcttcct tcgtgaagct tgttccaccg gcgttccaag tcgtcagtca
    23941 gagtttgttg gatcgggtca gcggtgagga gccaaaggtg cccacgacag agattgaagt
    24001 ttatgatcgg caaggcgtga tgaaatccgt ggagatggac ctcgttcgga ttgatatggg
    24061 aggaagagcc tcaatcatgg tccagttacg agatataacc gagcataaaa agtttgaagc
    24121 tgaactacaa cgatacaatg aagtcttgga agagaaagtc tctgagcgta ctcgggaaat
    24181 tgaacaagcc aaactgtaca ttgaaagttt gttggaaaat gcgaatgacg tgatttatac
    24241 cttggacgtt gatttacggt ttacttatgt caatggaaaa gttgatgcct ggggatatcg
    24301 aaaagaggac ttgattggta agccgtatct ctcgttactc tccaagcggt atcgtggacg
    24361 gtatttgaaa gagacattgg atctcgggac aaagcaagtc tacgaagtgg aggtggtgag
    24421 tcgagaggga gagttgcggg cggtgatggt gagtgtggca ccgcttctca atgatgcagg
    24481 cgtctacaca ggagtcttgg gcatcgccag ggatattacg gagcgcaagc atttggaacg
    24541 ccaggtgcag aattctgagc gattagcgtc cattgggaag ctggccgccg gggttgccca
    24601 tgaaatcaac aatccgttag ggggaatttt gaattgttta tacaatatcc gaaaagggac
    24661 gttgacgcct gagcgatctc aggaatattt gcattttatg gaggatgggt tgcgacgggt
    24721 tcaacgtatc gttcgccagc tcctggattt ttctcagcaa cgcgaaccgg aattagccat
    24781 gacagatctt catcaaattt tggaccgggt cctggtattg accgagcatg tatttttggt
    24841 caaacatgct acgttgagta agttctatga tgagacactt cctttggtga tggtcgatgc
    24901 gcatatgatt gagcaggtga ttatgaactt agtcctgaac gcggttcagg cactcaaaga
    24961 aggtggacag gtgacgttgc ggactcgaaa acaggaagat ggatatgaaa ttgaggttga
    25021 ggataccggg tgcggaatac ctccagagat tcgtcctcat atttttgatc ctttttttac
    25081 gaccaaaggg accggcgaag ggaccgggtt gggattgtcg gtgagtttgg gaattgtcca
    25141 gcgacatgca ggtgagatgt tggtggacag tgaagagggg aaagggactc ggtttaccat
    25201 tcgcttgcca ttggttcgat ctcgttcggg cattcctgtg aaggtaggca catgactcac
    25261 ggttcaatcc tgattattga tgatgagccc ttgatgcgag tctctatggt ggatgcgctg
    25321 aaggcgatcg gctatgtggt acaggaagcc agttccgggt tggagggact ggagcggttg
    25381 cggacttccc ggtttaattt ggtgattact gatttgcgat tacccggtgt ggacggcctt
    25441 catattgtaa aacagtgtaa ggagcattgc cccggcacgg aagtgattgt cataactgcc
    25501 catggctctg ttgacacggc cgtggatgcg atgaaaagcg gtgcttacga ttacatcacg
    25561 aaaccctttt ccatggatga attgttgttg agtgtggagc gagtctgcgc agtggtggct
    25621 cttcgggaag agaatcgggt cttacgggat gaattggaaa agaaatttag ttttcaaggg
    25681 attgtgggaa aaaacgagcg aatgcgacaa gtactggaaa agatcaagtt agtttcggtc
    25741 accgacgcga cggtattagt ggttggtgaa agtggaacgg gaaaagagtt gatcgcaaat
    25801 gccatacatg ccaatagcgc ccgccgaaat catgctttgg tcaaagtgag ttgtgccgcg
    25861 ctaccggaaa cgctattgga ggctgagctg tttggccatg agaaaggggc gtttacgggg
    25921 gctctccggc aacggcatgg tcgattcgaa cttgctcatc agggcaccct ctttttggat
    25981 gaaatcggcg aaatttcccc tttggtccaa gttaaattac ttcgtgtcct acaggaacgg
    26041 caatttgaac gcgtcggggg aaatgacacg attgaagtgg atgtgcgatt ggtgtgtgca
    26101 acacagaagg atttacggaa agaagttgaa caaggacgat tccgggagga tctctattat
    26161 cgattgaatg tggtgcccgt tcagcttccc cctctccgag agcgtcgaga ggatatattc
    26221 atgattgccc agcatttttt gaagacaatg tcaggtcgaa atcaccaaga accaaaagcc
    26281 ttgtcacggc aagctcgcga ggtgttgttg cagtattcct ttccggggaa tgtcagagaa
    26341 ttagaaaata ccattgaacg tgcgttagct ttggcacaac acactcaaga aatccaggtt
    26401 tgggatctct gtgggcagag tcgatgtcca tatgcgggtg gggaaccgca aaaaggatgc
    26461 gggctttgcg gggaccagga agggaggaga ggcgtgaaga acggggcgat ggaaaccttg
    26521 gccgaagcgc gagaaaattt tgaacggggg catattctcg aaatacttaa tcgaacgggg
    26581 tggagtcgga tgacggcttc gagggttttg ggattatcca gaaaaggtct ttgggaaaaa
    26641 tgtaaacggt atgggattgt tcgggcatgc gacgacacga attctgagaa ggacgatcgg
    26701 gatgacgatt gaaagcgcag cccttttttg aaaagagctg ccccatgagt gcttgcccct
    26761 tagggtttct tagtcggaaa atagattatt ctccaccctc ccagagggtg atgatccggt
    26821 cgtttccagc cgtggcaagg tatttcccat cagatgaaag ggtaatccct ctgacaggtc
    26881 ccttgtgtga ccgaatcgtt ctgaactgac gcccagtttg aatatcccac attttgacgg
    26941 tcccatcatc actggcgctg ataagcacct gtccatcctg ggtcacaaga agatttctaa
    27001 cccatgccga gtggcctttc agtgtgcggc gttcttccat cgtcggcatg tcccagaact
    27061 taatcgtaca gtcgcgactt ccggaaatca tggtgccccc atcaggagtg aaggtgagcg
    27121 ccccaatcca atattccatg ggcttttgaa atccgccctg gtcgtctacg taaaaaccac
    27181 gactttcggt cgattcgtcg tcggcatcat ctctccaatg gccgttgtga acaattcttt
    27241 cttccccgct tggtagaact tcatagagct taattttccc gacatcggta ccagcaacta
    27301 ggtggatacc gtccggggaa aacgctgtgc agacgctcca attatggtca tattggtctt
    27361 tcccaaggag cgtcatgagt tcagtgcctg tggttacttc ccagatttta atgtctttat
    27421 tctggccagc cctagcgagc atgaggccat ctggggaaaa tgaaatgctg gtcacgccat
    27481 ggtcgtaacg agtaaagagt aatcgaagtg attcccctgt ttgaggattc caaagccgaa
    27541 tggtccggtc ttcacttgct gaagcgatca tttgcccatc agggctatgc ttgacctctc
    27601 gaacggtaag ggtgtggcct cgtagagatc gaagaagacg gccggtttca atatcccaaa
    27661 tcctaataaa acgatcattt cctccgcttg ctaatgtcag gccatcaggg gaaaaggaga
    27721 cagaccatat ttcctgtgtg tgaccacgaa atgacttcag ttccctaaca ccgcttttcc
    27781 aataggcgag tgaatcaatg agtggcgtgg gttggaaatc tggattatgg atgaacccgg
    27841 gattggaact tagtgtgtca ggacttgtcg gctgggtcat gaaattcctc gcttcggaaa
    27901 cagattgaca ataaatggtc ggttatatga cctcgggaat ggatcaaggg aattctcttg
    27961 ccaaagggga aaactcattc ctataattca actagttatg atactaaacg gcgcttttcg
    28021 agggagtcaa gatagctacc tcccaaatcc aatgcgaatt tataattacg gtgatcatct
    28081 ttcgagaaat gaaactcgtc ttgtggtgat cttgcggtaa acggaggttt tgctcatgga
    28141 aattcgtttt caacccgccc tccttcagga ggtgattgac tcgtttgctg aaaagactga
    28201 gcgtgagggc gatccgactt attttaatga gtttcatgag tttgcggatc ccatttacga
    28261 gaagttttct cttgatgacc gggatccaga gttcaagcgg ctctatcaac atttatttgc
    28321 taaatggggg tttgctgaaa ttctcaggga tgcttttgat gatttcccgg ttttgcgaga
    28381 taaaactggg attgtattgg tgcgaggggt attaaaagaa gatcaggaag gggttgatgt
    28441 cctccggaaa tggggggtgg tcgaggagaa attatcccgc aaatttgagg aggccgggaa
    28501 aaaaggggta ggaattaaat tgatccccag gcgattttat gatccagcca ttacccgtta
    28561 cctacgacat gagttaacgc atatttctga tatgttagat gaggcctttg gttacgatcc
    28621 tgacacaaaa gtggggttga accccgggga agaacatttg attttaaatc gttaccgtgt
    28681 gctgtggaac cttcatgtcg atagccggat tgcccgaaca ggaaaagagc cgatgttttc
    28741 caaggaatat cggtttcggg agttccgttc ctggtatcga aagattcttc ccggtcaggt
    28801 ggaatccgtg tttgaaggga tctggcaggc ggattatctc acacatgctg aattagtaga
    28861 gatggcttcg gatacgatac gagttatcga gagagctatt gaggttgagg acagtgaagt
    28921 tcctgatgtg ccaaccaagc ccatgcttct cccgggattt ccttgccccc tttgtcgatt
    28981 cccaacctat acctgggtag aaaacatgga tgagacccta gaaggctttg tcttagatta
    29041 tattcgcgaa aaccatccag gatgggacgt ggagtatggg gcctgtgaca gatgtgtcga
    29101 ggtgtataag ctgcgggctt ctggagtagt ataaattctt aaaatgcctt tcctcttgtc
    29161 caatttggtt tatacgctat tgaaagtggt ataatggcgg aatccgataa gtcaagacag
    29221 ccttcatcta atccgacatt aggtcggaga atgtttcttc gacaatcggc aatttccatt
    29281 ggcgcgactg ttcatgaatt tgtgaagcac agagatgctc cttccccgat aaaaaaggaa
    29341 caacctgttc ctgaaaaaac ggggtggctt cgtcccccag gggccgtgga ggagaagttg
    29401 tttttggagc gctgtaccgg atgcgctgat tgtgtggaag tctgtcctca cgacgccatt
    29461 caagtgttat cgagtaatgg cactcccgtt atctatcctg gagaatcccc atgcaaatta
    29521 tgtgatgatc ttccttgtat tggggcatgt actacggaag cccttttacc tgttgaggat
    29581 agttttcagg tcaatatggg cgtggcgaaa gtttgggcaa aaatgtgtac ggcaggaacc
    29641 ggctgcaatg cctgtgtttc taaatgcccc actgatgctc tctcgatgga tttttcctca
    29701 tatcacattc gtgtagatga ttcagtatgt gtgggttgtg gaatatgcca atatatctgt
    29761 ggcacggtaa atgaccgtac agctattcga attgttccca ggtaatgggt cga
//
LOCUS       DAORAQ010000020        27015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_345_length_27015_cov_66.297255, whole genome shotgun sequence.
ACCESSION   DAORAQ010000020 DAORAQ010000000
VERSION     DAORAQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 27015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27015
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_345_length_27015_cov_66.297255"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKM72_03505"
     CDS             <1..781
                     /locus_tag="PKM72_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59879.1"
                     /translation="GLIGNEEELFATLQRLLGQTRLFTVGIGSAPNGHFMRKAAQHGR
                     GTFTYIGTAQEVQDKMHRLFVKLEQPAFLNLALEGSTEGAWDLLPAPLPDVYAGEPLM
                     AAFRSKTPPAHLTISGMHGTVPWKRVLPFAAGLSRSGIAVHWAREKISQLMDQQASSP
                     QPGKSDQETELRQAVIDVALRHHLVSKYTSLVAVETNPARPEHRPLQSHAMKTNLPHG
                     MQYEAILGWPQTATPSTLYLLLGIFMVGMGCLWKRQQAART"
     gene            790..1386
                     /locus_tag="PKM72_03510"
     CDS             790..1386
                     /locus_tag="PKM72_03510"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HNP59880.1"
                     /translation="MRRVLASLMLVLGIWLIGQGLWIPMKAIAAQWLLQQAWSKTLKT
                     QQPAKPWPWADTWPVGRLIVPQHGIDQIILADASGQSLAFGPGQLSESIKPGEPGSIV
                     VSGHRDTHFQFLQYLQEKDLIYVEWPTGHRTAYIVQELTVVDSRHSRLANHDDAETLI
                     LTTCYPFDALIPGGPLRYVVKAEKILAMENRSRVEMNN"
     gene            complement(1536..2009)
                     /locus_tag="PKM72_03515"
     CDS             complement(1536..2009)
                     /locus_tag="PKM72_03515"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNP59881.1"
                     /translation="MSPEPDKKGAAVKVPPPLIVLVVLLLAYALDYVVPLRIPQSSVL
                     DFAGYALVGISLLLIVITSRSFRRLNIHIEPWKPTTKIITTGFFGISRNPIYLALCAM
                     CIGVGMILHSWWVLFGAIPAALLLYHFVIAREEAYLEQKFGEAYVQYKARVRRWL"
     gene            complement(2524..3657)
                     /locus_tag="PKM72_03520"
     CDS             complement(2524..3657)
                     /locus_tag="PKM72_03520"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNP59882.1"
                     /translation="MKSRGHTIVIARNETPKIVDKGRGNFIRVLRARVDDHFAGRNRR
                     DDLRLYLKSAIVLIWFFISFGMLLASSNGAFQLLLCVSTAFAACGVGFNIFHDSIHNS
                     FSANPTVNLFLARSSCAMLGVSRYFWRHKHNVLHHSYTNILEWDDDLETRGALRMSPR
                     QAWEKKFKHQHLYCWFLYALSTIEWIFIKDFVHYFTMRINQFQKYPSMSSRDEIEFWT
                     FKAMYFSVFIVTPFLFQPFWKCVVGLVIFHLTLGLSVTLIFNLAHIMEKSAYPKSRGE
                     NPPNVESEWAVLQLATTVNFGRNNQCLTWFSGCLNYQIEHHLFPKVSHTHYPEISKIL
                     RRTAAEFHIPYNDFPTYLSALKSHFRTLKALSELPRLGVHPVP"
     gene            complement(3821..5410)
                     /locus_tag="PKM72_03525"
     CDS             complement(3821..5410)
                     /locus_tag="PKM72_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59883.1"
                     /translation="MRPRRVTILDLVTKGPTNSLYARVMNQNLASIMPQVVGVWCEEL
                     GHQVRFVCYTGREDLHRELLDETDIVFIGAFTRSALTAYAISSMFRARGAVTVLGGPH
                     ARSYPQDAAKYFDYVVGFTDKNLIQDILVDCAQHRPLGMHLAAPRQPTELPGVKERWK
                     FVEPTIAKAPTSFKVVPMIGSMGCPYTCGFCVDANVDYQPLSFDQISEDLKFLRTKLK
                     RPIVAWHDPNFGVRFQEYMNAIETAIPPNSIDFIAESTLSLLAEPHLKRLKQNGFKAI
                     LPGIESWYDMGDKSRTGRHTGQDKVRQVADHINLILRYIPYVQVNFVLGLDSDKGPEP
                     FELTRKFLDLAPGAFPAFSLLTAFGQAAPFNRELQRDGRVLPFPFHFLDNNHAMNVRP
                     KHYAWPEFYDHVVDLSRHAFSWPMIAKRVMLNRGWVPRWFNFIRSVSSEGFGRIKYHT
                     KIRGLMDTNVSFRKFLEGETSELPMFYARKIRGKLGSLYDMLPEGATMHDHHAYLNSY
                     QDPIPPLVALNPLPKVESAAI"
     gene            complement(5927..7324)
                     /locus_tag="PKM72_03530"
     CDS             complement(5927..7324)
                     /locus_tag="PKM72_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59884.1"
                     /translation="MRKLLRTASFRLTLFTACLFAVFVITTFVVSYWIAVRYLLERVD
                     GEITWQIQMLVKEYQAGSLNSANPDLVEPGTYLLLRDSAGRSIVQNLPSGAPVRKWFQ
                     ELPPKEIPIDHASHKLRAQTVLLPDGTQLTVGQDLRDLEAAKEFLRQAFGWLLAAILI
                     LGLACGAIVSAITLRRVEEINLTVGDIINGHFDRRISLKGTHDEFDRLSRQINLMLAQ
                     IEKLMGGLRQVSIDMAHDLRTPLSRMRQKLEGARNRATSLEDYEQAIDQALKQNDEIL
                     ETFSALLGIAQLGSGAYRQKFHEISLSEIFEILEETYAPIAEERHQILFATIQPDLQF
                     HGDSRLITQMLVNLIENAIRHSPSGSRISLILSQRMSGMTAVITDTGPGVPESEREKI
                     FQRFYRLESSRTTTGSGLGLSLVSAIADLHGIRISLSDNHPGLRVTLEFPLPISQSYK
                     KPSTLPIRNNLKQLR"
     gene            complement(7317..7994)
                     /locus_tag="PKM72_03535"
     CDS             complement(7317..7994)
                     /locus_tag="PKM72_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP59885.1"
                     /translation="MKILVLEDDQETASYIASGLIEEGYVVDIATDGKEGFKKAIDEV
                     YEVLIVDRMLPSLDGISLVKGLRTTNVKTPVLFLSALGGIDHRVEGLQCGGDDYLTKP
                     FAFSELKARVEALRRRPPMSMIETRLRVGDLEMDLLTRIVKRGEVCIELQPREFRLLE
                     YLMRNAGQVVTRTMLLEHVWDFYFDPRTSVVDTHISRLRMKINKGFTQQLIHTVRGTG
                     YRMSADA"
     gene            8533..10371
                     /gene="typA"
                     /locus_tag="PKM72_03540"
     CDS             8533..10371
                     /gene="typA"
                     /locus_tag="PKM72_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNP59886.1"
                     /translation="MLNQKRRKDLRNVAIIAHVDHGKTTLVDALLRQTGAHVFKEGEA
                     VIMDSNPLEKERGITIFSKNASLLFKDTRINLVDTPGHADFGSEVERILRMVDGVLLL
                     VDAFDGPMPQTKFVLKKSLELHLKPIVVINKIDRPSARPEEIVNQTFDLFCELNATDE
                     QLDFPIVYASGKEGKSTLDLNDPAVDMKPLLDTIIHRVLPPVADPEQPFQMLVTMLEY
                     DSYIGRVAVGRIVHGMIEVGNQIVAVKRDGTISRGKVTKLLAYQGLTRVETGSAQAGD
                     IISLAGHQDVRVGETLASPQNPTALPTVNVDEPTISMNFSHNTSPLAGKDGGRFLTSR
                     HIRERLAHEAMMNVGIKVEEADGGERFTVSGRGELHLSILIETMRREGFELEVSPPKV
                     IYKEVDGETLEPVELVVIEVDPGYQGAVIEALGSRKAEMKDLRISAVGTVRMEFHIAS
                     RALLGFRGELLRMTRGSGVMSQIFYEYQPFKGEIPHRSTGVLIAHGPGQAVAYGLWGL
                     QDRGEIFLHPGVDIYEGMLVGVNNKGNDLVVNALREKKLTNIRASGTDEAIHLIPPRE
                     MTLEFALEFIEDDELVEVTPKNVRLRKRYLTDNERRSARNMSKKAQ"
     gene            10818..11687
                     /gene="dapF"
                     /locus_tag="PKM72_03545"
     CDS             10818..11687
                     /gene="dapF"
                     /locus_tag="PKM72_03545"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNP59887.1"
                     /translation="MNNTFFKGHGLGNDYIALDPAKMSFKLTPRTIRALCDRHWGVGS
                     DGILALGPSKKADFGLRIYNPDGSEAEKSGNGLRIFGCYLYHTKQTRKKHFTVETKGG
                     LVEIHLDLNGHGFVNGATVDMGRASFQPISLPCTLRVPELIQQPVKAAGQSLRFTGVS
                     VGNPHCVVYKKQEEQWTRQDLLEIGPELENHIIFPKRTNVQLAIPTGPRAISILIWER
                     GAGETQASGSSACAAASAGVRLGLVKSPVRVKAQGGTLDITVDSQYNITMKGPVTEVA
                     RGEISQSFINGLR"
     gene            complement(12107..13132)
                     /locus_tag="PKM72_03550"
     CDS             complement(12107..13132)
                     /locus_tag="PKM72_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNP59888.1"
                     /translation="MMGLFMIASLAMGSEPGFDSSKGMLTVDGHVVPDIGPLPTVVPT
                     PPTNLNYAAKISLGKQLYFDGRLSKNNAISCAFCHNPVTGFADPNQTSAGVGGLRGGR
                     QSPTVYNTAFNPFQFWDGRAGSLEEQAIGPIHNPVEMAETHESVVPKIAKIKGYEEEF
                     QKVFGTGVSLQGIAEAIAAYERTIISTDSTFDKFVLGDPKAMGEDAKRGMDVFKGKGR
                     CILCHNGSNFTDNQFHNLGVPQVGPMKEDLGRYYVTLRERDKGAFKTPTLRSITESAP
                     YMHDGAFKTLEEVVDFFDEGGKANPQLSPLMKPLGLTPQEKTDLIAFMKALTGEPIPF
                     EFPTLPE"
     gene            13439..15961
                     /gene="hrpB"
                     /locus_tag="PKM72_03555"
     CDS             13439..15961
                     /gene="hrpB"
                     /locus_tag="PKM72_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNP59889.1"
                     /translation="MKRPSYPIDAVVPQLRQTLNQQPIVLLTAQPGAGKTTQIPLALL
                     QEPWLTPKTIIMLEPRRLAARAAARRMSDLLGEAVGTTVGYRTRLDTKISPNTKLEVV
                     TEGILTRILQHDPSLESYGLVIFDEFHERSLQADLGLALCLESRKVFREDLRLLIMSA
                     TLDIAAISQQLKQAPVVTCEGTMFPVETRYVGKPDVKNFAIQVAQTIHRLLKTEPGNL
                     LVFLPGAGEIRRIERILADLPLGSHILIAPLYGDLSAQAQDQAILPPPPGWRKVVLAT
                     NIAESSLTIEGIRLVIDTGLMRIPRFDSRSGMSRLTTLTVSQQSAEQRRGRAGRLEPG
                     LCMRLWSEAEQRTFTPRTAPEILDADLTSLALELAQWGIQDPQELLWLDSPPSGAMAQ
                     ARQLLHSLGAFDAQGHITDHGRSMAELPMHPRLAHMVLKGKELDSGALACDLAASLSE
                     RDLFKGSTAREHADLRARFDVLYGSAHAQRDTGFFNRGTLQRIRQVSQAWQRTLHITT
                     PRHVPKQQIDQMGVLLALAYPDRIAQRQSDEARRYRLANGRSARFHHPDPLEHEEWLV
                     IADLNGAPATALIYMAVPISREDLITHCGDLIQSTDSVMWDGSTQTVRSIRQRRLGEL
                     ILEESRLPDPDPDLVLTVLLEGLRNTGLPCLPWTPTLRNWQARVQFLRRVTEPGSTWP
                     DVSDDTLLHTLDQWLGPYLTNLSSLNQLKRIDLAWPLQALLSPEQRQTLDTLAPTHLT
                     VPTGSHIPLDYLSGEIPVLAVRLQEIFGQCDTPRLVNGKVPVLIHLLSPARRPVQVTQ
                     DLTSFWATGYQAVKKELKGRYPKHFWPDDPLQAPPTRGIKKR"
     gene            complement(16099..16893)
                     /locus_tag="PKM72_03560"
     CDS             complement(16099..16893)
                     /locus_tag="PKM72_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNP59890.1"
                     /translation="MKHLVLGIRMTRYLVSVFLISGFLGFGQTVQANEFSTWDALLKK
                     YVASKTIEGITLYAVNYQALGHDPLYRRVIADLEKAPVEGFSTQEEKLAFWINAYNIM
                     AVKMVLDHYPLKSIKDAGGLFSSVWKLNVGTVRGKARTLNEIEHDILRKMGEPRIHVA
                     IVCASLSCPDIRPEAYTSERLHEQLDDQMKAFLANPNKGLQVEESNHRLYLSSIFKWF
                     AEDFESKGGVRPFLAMYVPERVSAYLKNETFRLRYLDYNWNLNAFK"
     gene            17140..17946
                     /locus_tag="PKM72_03565"
     CDS             17140..17946
                     /locus_tag="PKM72_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59891.1"
                     /translation="MNANLPSQIIVVAGWVLGSFFLGCSSVELTKVPKKPLPYHPPPL
                     VELPLELPPEGGPRRPVDAMSPTTLSAPGPEIFIVDSNQPGLRATAERDPDVKTQLGE
                     RFAYINTEKVPADQCLVILQGDDSSNSRTTERASNSDASAYRLTYYSYTHNVAVHVCM
                     ENDRMSSVQQDPREGYQPEEGEEEITAAIELARADQRIAQEIQHLHGHAILTSPEEYR
                     YFWVSDEAGFGDRVLWVTFSEMPESSALYFARVDLTSQTVLDAGKEAGPQ"
     gene            17943..18956
                     /locus_tag="PKM72_03570"
     CDS             17943..18956
                     /locus_tag="PKM72_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59892.1"
                     /translation="MTIPKQEVGRMASFRYFIVGVALTSVLAVSPALADWESKPFTWG
                     PWDLHLEMKDAAGLGLRNVSYEGKQILGKADLPVIRVKYVREWPAWHPFSWFGLGRSS
                     GRCGPFEDRISPPRLKKNPNCDGKQLCQAEHTIQGVKWLELGVYAKIGQYHLYQAWYL
                     SEDGRLYPSVQSWNLSCNTDHDHHAYWRLDFDIGGSDQDQVFVLDRNSTNDNGWGPGW
                     EKYLVEEDAKKPASNSQDRVWFVRDYPTGQGVWIIPGPFDGHSNNFSNRDVSIRKFYR
                     DEDTGWPFGARGDLESKSDESVQETNVVFWYVAHLPHKAAEGDRPMRYWMGPLLQIYQ
                     ESP"
     gene            19212..19778
                     /locus_tag="PKM72_03575"
     CDS             19212..19778
                     /locus_tag="PKM72_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59893.1"
                     /translation="MKLLSRILCIRACSLLAYSSILLFGPLGLAEPGIDGFRDLKFGM
                     TEQDVSALPACKSSTECLYELTDKNRYLELTYLPDTVPGTPEPVRKLAKITIDMGQFT
                     EDWFEQLQIILGDSYHLTQDLTEPTKQSFLAGKQNELNLGFEEGQVLLKVVRRPFGNL
                     ALKVVYQNAALAKGFIQQSQVAATASSN"
     gene            complement(19861..20253)
                     /locus_tag="PKM72_03580"
     CDS             complement(19861..20253)
                     /locus_tag="PKM72_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59894.1"
                     /translation="MRRMLTWDIPESVMTQDQAWNPKRKYIAESVGLVTHRASRGSGI
                     GITCCAKCGSEVKVFVSPRRNPHSSKLGRAVSLKDHDLCRRCWRKLMHQQRKTGVVTL
                     PISFFVKEESLRPRLLVPTIASVPKQAS"
     gene            complement(20634..21077)
                     /locus_tag="PKM72_03585"
     CDS             complement(20634..21077)
                     /locus_tag="PKM72_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59895.1"
                     /translation="MTNKAILLVEDHPDDEELTMRALKKNNIKNEVVVARDGVEALEY
                     LFGTGAHAGRDLSNMPQIILLDLKLPKIDGLEVLRRLRADDRTKFLPVVVLTSSKEEQ
                     DIVKSYQLGANSYVRKPVDFQEFSQAVQNLGLYWLILNESAPERV"
     gene            complement(21098..22750)
                     /locus_tag="PKM72_03590"
     CDS             complement(21098..22750)
                     /locus_tag="PKM72_03590"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP59896.1"
                     /translation="MELLHQHTILLLSVLFGLCLIVIYLHISQLKSQMVTVMAFRGSE
                     IYTRALNEMRIWYTVEVVQRLHAQGIEASEGYRDHQGEIPLPATLSRDLGRRLVAGRP
                     GEQLKLMSPYPFQNRVKSGGLMDSFQGEAWEFFAQHPDSDEPFYRFEQVDGRESLRFA
                     KADRMQKECVECHNRHPDSPKRDWQIGQVGGLLEVVYPINSVETIMGMSWLGTYGLMG
                     MLVVIWMGGFWLVVLKLGRVASELEEEVRDRTGALQSVNKHLELEIHDRQLAEDTLRQ
                     AKDNLEKRVQERTGKLAASNAELIREVAERKRAEEDIRKLNSQLVQRSAQLEASNKEL
                     EAFSYSVSHDLRAPLRGIDGFSQAVLEDYDEKLDESGRSYLRRVRTASQRMSQLIDAM
                     LNLARLTRAEIHTQTFDMSAVVRGILDDLQKVEPDRQVECIVANDVFATADPQLLRAV
                     LENLLGNAWKFTQQQAHPRIEFGYGQYKGQAAYFVTDNGAGFDMTYVHKLFGAFQRLH
                     AYTEYPGVGVGLATVHRIIQRHGGQIWAEGVVGKGATFHFTL"
     gene            complement(23500..25824)
                     /locus_tag="PKM72_03595"
     CDS             complement(23500..25824)
                     /locus_tag="PKM72_03595"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNP59897.1"
                     /translation="MELRPQEWFYTLRQEYLQRFIGQGGSSVKFVVTDSDPDRVDVQN
                     QLAVLSQQEGYACISVDAKDTKIHMMDKFFHQIARRIPWDDLASQFVRRLLQENGYQI
                     PENKEEFCMAGVARMNERAEPLLRRDLNSWLERAIYRDTQMCQEFRMAMIRLCLGQMD
                     SGSEVPFMTEPVKSWLGGEIRQISALKEALIFQKINRTNARYMFVSLCRWLRLVGITG
                     LVVSLDLSRCLLSKKPDDPEGLYYGVSATLDAYEMLRQFIDGTDELESFLLVVHVPQE
                     FLTDERRGLNRYEALKLRIWDEVRDRQYQNPLGALIRLGSQQPGDAHDTGKREYNDRP
                     AMANGDVGHQRAMEALRSGVPNRDVVQVLGSHQPDLEGKFRRLIQQMEANVPHEMPTK
                     GIVIEGGFGSGKSHLLHALQQVALEQNFVCSPIVISKETPLYNGVPFFRAAINNAIVP
                     GKHGDALTEIAGELNFQSPQYADLFDWVHRKDQVCDSRFAASLYLYERMINDPELSHR
                     MIRYWAGDPISNSQLNKYLQGCPPENPYVFNKISSQDLALGRFQFVSRLMVAAGYKGW
                     ILLIDEAEIIGRYSFKQRTKSYMEVARWMGVLADHSCPAIGAIVALTDDFQSVVLEEK
                     QDSQKIEQMCQEESTDEAHMQALQAVQGIRLIEQEGEPLVRPYESMVDALYERLRTLH
                     GSAYSWTPPPVSAVEKLSSTRMREYVRGWITEWDLRRLYPDAQLDIEILDVRQTYDED
                     QELEPSMGDDTDPGKISESEECLMAVALQNQAPV"
     gene            complement(26006..>27015)
                     /locus_tag="PKM72_03600"
     CDS             complement(26006..>27015)
                     /locus_tag="PKM72_03600"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNP59898.1"
                     /translation="TPLYSPALMFRSAIESAEIPEKRGDVLAEVAADLNFKSPQYANF
                     YEWVHRQGGEIDGRFAATVFLYERMVNDPELSHRIIRFWAGDPLTDAEIKRYLKSCDA
                     NVSYRFERVSPMEMALQRFKFVSRLMIAAGYSGWILLIDEAEIIGRYSFKQRAQSYAE
                     LARWLGRLEASSFADLGYKSGLAAVMALTDDFQSAILDEKGDREKVPEKLRETGSETD
                     LVLSRQAEQGMRLIECERIPLVGPYDQLVEEIYHKIRSIHGAAYGWEPPQVPTIERLS
                     STRMREYVKGWITEWDLTRLDPTQKVEIELTEIRQDYSEDRELEGDQEGILHPALSES
                     I"
BASE COUNT         6835 a         7335 c         6280 g         6565 t
ORIGIN      
        1 cggattgatc ggcaatgagg aggaattatt cgcgaccctt caacgtctcc tcggccagac
       61 ccgcttgttt accgtcggca tcggctcggc ccccaacggg catttcatgc gcaaggccgc
      121 ccagcatggc cgcggcacct ttacctacat cggcaccgcc caggaggtgc aagacaagat
      181 gcaccgcctg tttgtcaaac tcgaacaacc tgcatttctc aatctcgcgc tggaaggttc
      241 caccgaaggg gcatgggatc tccttcctgc tccacttccg gatgtgtatg cgggcgaacc
      301 gctcatggcc gcgtttcgat caaaaacccc tcccgcccat ctgacaattt ccggcatgca
      361 cggaaccgtt ccttggaaaa gggtactacc gttcgcggcc gggctttccc gttcgggcat
      421 tgccgtccat tgggccaggg aaaaaatatc ccaactcatg gatcaacagg cttcatcgcc
      481 ccaacccggc aaatcggacc aggaaacgga actccgacag gccgtcatcg acgtggctct
      541 gcggcatcat ctcgtgagca aatacacaag cctggtcgcc gtggaaacca atccggcgcg
      601 gcctgaacat cgcccactcc aatcccatgc gatgaagacc aatcttccgc atggcatgca
      661 atacgaggca atcttgggct ggccacaaac agccacaccg tccacgctct atcttttgtt
      721 aggaatcttc atggtcggga tgggatgctt atggaagcga caacaagctg cacggacgta
      781 ataccgcgtg tgcgtcgagt attggcaagc ctgatgttgg tcctgggaat ctggttgatc
      841 ggacagggac tctggattcc catgaaggct attgccgccc aatggctttt gcaacaggcc
      901 tggagtaaga ctctcaagac acaacagccg gccaagccct ggccatgggc ggacacctgg
      961 ccggtgggac gactgattgt tccgcaacac ggcatcgacc agattatcct ggccgatgcc
     1021 agcgggcaat cgttagcctt cggaccagga cagctttccg aaagtataaa gcctggtgaa
     1081 cctggcagca tcgtggtgag cggacatcgc gatacacact ttcagttcct gcaatatctt
     1141 caggaaaagg atctcatcta tgtggaatgg cccaccggac atcgaacggc ttatatagtt
     1201 caggaactca ccgtggtgga tagccgacac agccgccttg ccaatcatga cgatgcggaa
     1261 actttgatct taacaacctg ttatcccttt gatgctctca taccgggagg accactcagg
     1321 tatgtggtga aagcagaaaa aattctagcg atggagaatc gaagtcgtgt agaaatgaac
     1381 aattaattct catgacagaa tatgatgcat aacatgagaa acaggtaggt atggcaagcc
     1441 taacggatgt gttccggcac atgacgggct ctaataacgg cgcaaaaaat atgactcaca
     1501 tcgctgttcg atgagatcat agccagcgga tgagatcaca gccagcgtct gacgcgcgct
     1561 ttatactgca cataggcttc cccgaatttt tgttcaaggt aggcctcttc gcgtgcaatg
     1621 acgaagtggt agagcaacag ggccgcaggg atcgcgccga acaacaccca ccaactatga
     1681 agaatcatgc cgacaccgat acacatggca cacaacgcca gataaatcgg attgcgtgag
     1741 ataccaaaga acccggtcgt gatgatcttc gtagttggct tccagggttc aatgtgtatg
     1801 ttcagacgcc ggaatgacct tgaggtgata acgatgagta agagtgaaat cccgaccaat
     1861 gcatacccgg caaaatctaa aaccgatgac tgtggaatcc tgaggggaac cacgtagtcc
     1921 agagcatagg ccagaagcag gacgactaga acaatgagtg gcggcggaac cttgaccgct
     1981 gcgccttttt tgtcaggctc agggctcata accacactga aatataatgt gggtaaagga
     2041 tgttgaagcc tttagatggt ctcttcatgg atttgaggat gtcttcactc ctgggggcgg
     2101 gaaagctatc aaccgttcct acctcccttg gtctcctctt acaaaggagg gataattgaa
     2161 gaagaggaaa agggtaaaag aataatgccc aaactcggca ctaaattaaa acggcccagt
     2221 gtttccaatg gtgaattttt ctttcagaat cgatgtggct tccctcacgt tcaggtcctt
     2281 ctgcagcact tgtgcgaatg atcgcatttt ataggtcttg gactgtgggg attcaacctg
     2341 tcccgccttc ccccattccg tcactcacac atccgccgca caacgattag gtgattcctt
     2401 ctggtttcgg cggggttgcc ccaacacacc atcaaaattt gtgaccccat gatgcgttct
     2461 ttcatcatcc tactcctact cacccattag ggcctggatc tttcaggaag aggagctagc
     2521 tctttaaggc acaggatgaa cacccaagcg gggtaactca ctcaacgctt tcaatgtgcg
     2581 aaagtgactc ttcagtgcgg aaagataggt ggggaaatca ttatatggaa tgtggaactc
     2641 tgcggcagtt cgtctgagga ttttgctgat ctccggataa tgcgtgtgac tcactttagg
     2701 aaatagatga tgctcgattt ggtagttcag gcaccccgaa aaccatgtga ggcattgatt
     2761 atttcttcca aaatttactg ttgtggctag ctggaggact gcccattcgg attcgacgtt
     2821 aggcggattc tctccccgcg atttcggata cgcggatttt tccataatat gagccagatt
     2881 gaagatcaag gtcacactga gacctagcgt aaggtggaaa atcaccagac ctacgacaca
     2941 tttccagaac ggctgaaaaa ggaaaggcgt gacgatgaac acggaaaaat acatcgcctt
     3001 aaatgtccag aattcgattt catctctgct cgacattgat ggatatttct gaaattgatt
     3061 gatcctcatc gtgaaatagt ggacaaaatc ttttatgaag atccattcga tcgtggacaa
     3121 cgcatagaga aaccagcaat acagatgctg atgcttgaat tttttttccc aagcctgccg
     3181 aggactcatc cgcagggccc ctcttgtctc caaatcatca tcccattcca gaatattcgt
     3241 ataactatgg tgcagaacat tgtgcttatg gcgccagaaa taccgactga cgcctaacat
     3301 ggcgcaggag ctccgtgcaa ggaacaggtt gacggtgggg ttggccgaaa aagaattatg
     3361 gatcgagtca tggaagatgt tgaatccgac cccacaggcg gcgaaggccg ttgacacaca
     3421 aagaagcagt tgaaacgcac catttgagct ggccaacagc ataccaaacg agataaaaaa
     3481 ccagataagg acgattgctg atttcagata caggcggaga tcatcacggc gatttctgcc
     3541 cgcgaaatgg tcatcaacac gggcccgaag aacgcgaata aaattaccac gacccttgtc
     3601 gacaatcttg ggcgtctcgt tcctggcgat cacgatggta tggcctcttg acttcatctt
     3661 tgagaaacct gcttgaattt ttcccgatta ttaaggttgg cgatctattg gtctgctgaa
     3721 tgggaaggct aagtggaaaa tcccagagtc acgcataacc ccgagtagcc atctgaaaga
     3781 ctgaggctga ggtatgcgat aatgcagtcc tgacttgttc ttatatagcc gcgctttcta
     3841 ccttggggag tgggtttaat gcgacaagag gaggaatcgg gtcctgatag gaattaaggt
     3901 acgcatgatg atcatgcatc gtggcccctt caggaagcat gtcatagagc gaacctaatt
     3961 ttccgcggat tttcctggca tagaacattg gaagttctga tgtttcccct tccagaaatt
     4021 ttcggaacga cacattcgta tccatcagtc ctctgatctt ggtgtgatac ttgatccggc
     4081 caaacccttc actcgacacc gaccgaatga aattaaacca tctgggaacc cagccacgat
     4141 tcagcatcac ccgcttggca atcatcggcc aggaaaacgc atgccgtgac agatccacga
     4201 catgatcata aaattccggc caggcataat gtttgggccg cacattcatc gcatgattat
     4261 tatccagaaa atgaaaaggg aacgggagca cccgaccgtc ccgttgcaat tcccgattaa
     4321 acggcgcggc ctggccaaat gccgtcaata gggaaaatgc cggaaaggcc ccgggcgcta
     4381 aatccagaaa tttcctggtg agttcaaacg gttcaggtcc cttgtcggaa tccaagccca
     4441 acacaaaatt cacctggaca taggggatat agcggagaat cagattgata tgatcggcca
     4501 cctgcctcac tttgtcctgt ccggtgtggc gaccggtccg ggacttatcg cccatgtcat
     4561 accacgactc tatacccggc aaaatcgcct tgaatccatt ttgttttaag cgtttcaggt
     4621 gcggttcggc caagagcgac agggtacttt ctgcaatgaa gtcaatacta ttgggtggaa
     4681 tcgccgtttc aatggcattc atatattcct gaaaccgcac cccgaaattc gggtcgtgcc
     4741 aggccacaat cggtcgcttc aactttgtcc gaagaaattt gagatcctca ctaatctggt
     4801 caaacgacaa gggctgatag tcgacattgg catccacaca aaacccgcag gtataaggac
     4861 accccatgct gccgatcatc gggacgactt taaatgatgt gggcgccttg gcaatggtcg
     4921 gctcaacgaa tttccagcgt tccttcactc ccggcaactc ggttggctgc cggggcgctg
     4981 cgaggtgcat accaagcgga cgatgctgcg cacaatcaac tagaatatcc tgaatgagat
     5041 tcttgtccgt aaaccctacg acataatcga aatacttggc cgcatcctgc gggtagctcc
     5101 gagcatgagg cccacccagt accgtcacgg ctcctcgagc tcgaaacatg ctactgatgg
     5161 cataggcggt cagagcggac cgggtaaagg ccccgataaa cacaatgtcc gtctcgtcca
     5221 gcaattcccg gtggagatct tcacgaccgg tatagcagac aaagcgaacc tgatggccta
     5281 attcttcaca ccacacccca acgacctgcg gcataatgct cgccaggttt tgattcatga
     5341 cccgggcata gagagaattg gtcggcccct tcgtcacaag atccaatatc gtcacccgac
     5401 gtggtcgcat ataaaacctc cataaacaca agggcttaga caggaacagt cccagaatgg
     5461 gggaattgat tattctagat gcctatgccg gacatgatga tttgaacatc acaccacaac
     5521 ccacatgggc ttttcataaa catacgagtt accaagtccc ttaggccaca ccatagacct
     5581 caattgaata catgggattc tcaggggcaa atccggtgcg atgaacttgc cttcagcaga
     5641 tatcgaattt cgcccatccc agtaccccct cacctaattc atgtgttgag agaggaaact
     5701 gaggcgaccc attccgggga aatcaaggat gaagtgaaat gccgtggatt gttcggaatt
     5761 ttcggggatg taaattttgg tatgagaaag tattctgatc gtcaatccga aaatcgtgct
     5821 cacttcgctt tttttattcc cctccttcgt accggtgcgt ataggacaat ctgataggaa
     5881 aaagatccga acgtgtcata gtccttcaat tatacactaa cgaatcttac cgaagctgct
     5941 ttaaattatt acgaattggt aaggttgagg gttttttgta cgactgggaa atgggcagag
     6001 gaaattccaa tgttacccgt aatcctggat gattgtcgga aagactgata cggattccat
     6061 gaagatccgc aatcgcagac actaaactta ggccgagtcc gcttccggtt gtggttcggc
     6121 tcgattccag tcgataaaag cgttggaaaa ttttttctcg ttcggattcc ggaacgccag
     6181 gtcctgtatc ggtaatgacg gccgtcatcc cggacattcg ttgagaaagg atgagagaga
     6241 ttctggaacc tgacggggaa tgccgaatgg cattttcgat gaggttgacg agcatttggg
     6301 tgatcagtct gctatcgcca tggaactgta agtctggttg gatggtcgca aagagaattt
     6361 gatgacgctc ctcggcaatc ggggcgtagg tttcttcaag gatttcaaag atttcactga
     6421 gtgagatctc atgaaatttc tgccggtagg caccagagcc caactgtgcg attcccaaaa
     6481 gggcactgaa cgtttccagg atttcatcat tttgtttgag ggcctgatca atagcctgct
     6541 cataatcctc cagagaggtc gccctgttac gcgctccttc cagtttctgt cgcatacgcg
     6601 acaacggcgt cctcaaatca tgagccatat caattgaaac ctgccgcaat cctcccatca
     6661 atttttcaat ttgtgccagc atcagattaa tttggcgact taaccgatca aattcgtcat
     6721 gggttccctt aagagagata cgacggtcaa agtgcccatt aatgatgtcc cccacagtga
     6781 gattgatttc ttccacacgt cgcaaagtaa tggcgctcac aatcgctcca caggcaagcc
     6841 ccaatatgag aatagcggct aacaaccatc caaacgcttg ccgtaaaaat tctttcgccg
     6901 cttcaagatc tcttaaatcc tgcccgacgg tcaattgggt gccatccggt aacaacaccg
     6961 tctgcgcgcg aagtttatgt gatgcatgat caatcgggat ttctttcggt ggcagctcct
     7021 gaaaccattt tcggacagga gcccctgacg gcaaattctg aacaattgat ctcccggcgc
     7081 tatcccgcaa cagaaggtaa gtccccggtt ccaccagatc gggattcgcc gaattgagag
     7141 atccggcttg atactccttg acgagcatct gaatctgcca ggtgatttct ccgtcaacac
     7201 gctcaagtag ataccggacg gctatccaat aggaaacgac aaaggtggtg atcacaaaaa
     7261 ccgcaaacag gcacgcggtg aaaagggtta atcggaaact ggccgtacgt agaagcttac
     7321 gcatcggcac tcatacgata acccgtgccg cggacggtat ggatcagttg ctgtgtaaag
     7381 cctttattga tcttcatcct cagacggctg atatgcgtgt cgaccacgct ggtccgggga
     7441 tcaaaataga aatcccatac atgctcgagc agcatggtgc gggtcaccac ctgaccggca
     7501 tttcgcatga gatattcgag caggcggaat tcccgaggct gaagctcaat gcacacctct
     7561 ccccgtttta cgattcgggt gagtaaatcc atttcaagat ccccgacgcg caaccgtgtc
     7621 tcaatcattg acatgggcgg acgacgacgc agagcctcaa ctcgagcctt taattccgaa
     7681 aaggcgaagg gtttagtgag ataatcatca ccaccacatt ggagtccttc gactcggtga
     7741 tcaatcccac ccaaggcact gagaaagagt actggggttt tcacattcgt agttcgcagt
     7801 cctttgacca ggctgatccc gtcaagactg ggtagcatcc gatcaacaat caagacttca
     7861 tacacctcat cgatggcctt cttaaaccct tccttcccgt ccgtagcaat atcgaccacg
     7921 tatccttcct cgattagacc actggcaata taggacgccg tttcctgatc atcttccagc
     7981 acgaggatct tcattaccct acgcaagcct ttatatttgg gatggggata acctcagcat
     8041 aacacggtaa agggccctgt ctcagctaaa atatcccggt ccaacacctg gagcgaacgg
     8101 gaaaacctct tccaccctat catgagacca ttttgcgttt atcggtttta cccgacgtca
     8161 gtccatggtc ttgaaactct aaagccacct aatttgcccg ggttcatcgc cttcgtttaa
     8221 atcggagcct aagtaggttc ggtgaatctc accttcaaga atttccttag gaaaggggaa
     8281 aaaatggccg tcaaacgttg agggggcttt ttgaaattta aggaaagtca aggaaaacca
     8341 gttgcattag aactgatctt cctgtacggt tttgcaagaa tcgaccgtct tgcaggatcc
     8401 aagccattct ccggctgttc tggccggtgc cgtagcccaa acctcctcag accggcctcg
     8461 atacgcaacc tcagcataga acaatgtcgt tcgtgcttcg tctggttgtg cccaacaaag
     8521 gaaccgactc tcatgctgaa tcaaaaacgc cgaaaagacc ttcgcaatgt cgccatcatc
     8581 gctcacgtcg atcatggcaa aacaacgtta gtggatgccc tgctcaggca aaccggcgcg
     8641 catgtattta aagaaggcga agcggtcatt atggattcaa atccattaga aaaagaacgt
     8701 gggattacga tcttttcgaa aaatgcttcc ctcctcttta aagatacccg catcaatctc
     8761 gttgataccc ccggccatgc tgactttggt agcgaggtcg agcgaattct gcgaatggtg
     8821 gacggcgtgc tcctgctcgt ggacgccttt gatggcccga tgccccaaac caaattcgtc
     8881 ctgaaaaaat ccttggaact gcatctcaaa cccatcgtgg tgatcaacaa aatcgatcgt
     8941 cccagtgcaa ggcccgaaga aatcgtaaat cagacctttg atttattttg tgaactcaac
     9001 gccaccgacg aacaattaga tttccccatt gtgtacgcat ccggaaaaga aggaaagtcc
     9061 accctggatt taaatgatcc tgccgtcgac atgaagcctt tattggacac gatcatccat
     9121 cgggtcttgc cgccagtggc tgatcctgag caaccgtttc aaatgctggt gaccatgttg
     9181 gaatatgatt cctatatcgg acgtgtggct gtgggacgga ttgtccatgg catgattgaa
     9241 gtcggcaatc agatcgtcgc cgtcaaacgg gatggcacca tttctcgggg taaggtcacc
     9301 aagcttctgg cctatcaggg tctcacccgg gtcgaaaccg gttccgctca agccggagac
     9361 attatttccc ttgccgggca tcaggacgtt cgggtgggag aaaccttagc ctctcctcaa
     9421 aacccgacgg ccttaccgac cgtcaacgtg gatgaaccga cgatttccat gaacttctct
     9481 cataacacca gccccttggc cgggaaggac ggggggcgct ttctcacgtc acgacacatt
     9541 cgagagcgcc tggctcacga agccatgatg aatgtcggta ttaaagtgga ggaggccgac
     9601 ggtggcgagc gatttaccgt ctcgggacga ggcgaactcc atttgtccat tttaatcgaa
     9661 accatgcgtc gggaagggtt tgaattagag gtatccccgc ccaaagtcat ttacaaggag
     9721 gtggacggcg aaaccctgga acccgttgaa ctcgtggtca ttgaggtaga ccccggttat
     9781 cagggtgcgg ttatcgaagc gctcggcagc cggaaagccg aaatgaaaga cctgcgaatc
     9841 agcgcggtcg gcaccgttcg catggaattc cacattgcct cgcgtgccct tttaggattt
     9901 cgcggtgaat tattgaggat gaccagagga agcggtgtca tgtcacaaat tttctatgaa
     9961 taccagccgt ttaaaggaga gattcctcat cgttccaccg gcgtgctcat tgctcatggt
    10021 cctggacagg ccgtggccta tggtctctgg gggcttcaag accgtggaga aatattcctc
    10081 catcctggag tcgatattta cgagggcatg ctggttgggg tcaacaacaa aggcaacgac
    10141 ctggtcgtga atgcccttcg cgagaaaaaa ctcaccaata ttcgggcgtc tggaaccgat
    10201 gaagccattc accttatccc gccacgggaa atgaccttag aattcgcgtt ggaattcatc
    10261 gaagatgatg aactggtcga agtgactccc aaaaatgtgc gcctgcgtaa gcgctacctc
    10321 accgacaacg aacgccggtc ggctcgcaac atgtccaaga aggctcaatg agctccacct
    10381 tctcctcgtg attgatctga tattttggcc tcgctttcac taacctgtgg cagcgaggcc
    10441 catggccctt ccctcccatt caacccctaa ggcttgcgag caaatggact gttcgatgtg
    10501 ctcggaaaag cattgaaccg atctgaggtg ggacaccgcc cccttgtgat ctttttccac
    10561 catacttcta ataaatggct tgcgcgaact cctggttgca tcctctccat catcaagata
    10621 gaccggctcc tgttcttatc aggcaaatgg ttggctaatt cgctataaga ttttgccttc
    10681 ttcgacgttt tctcccctta agtgctttcc tctttcaaca tttttacgta taattcaggc
    10741 cctaccgtcc agccgggaaa aagccttgta ggcagggccg atcacccgat agcagtccat
    10801 tccattgtgg agcaggcatg aacaacactt ttttcaaagg acatggcttg gggaatgact
    10861 atattgccct cgacccggcc aaaatgtctt ttaaactcac accgcggacc atccgggctc
    10921 tttgtgatcg gcattgggga gtaggaagtg acggcattct ggcgctcggc ccttccaaaa
    10981 aagccgactt tggtctgcgc atttacaacc ctgacggcag tgaggcggaa aagtccggta
    11041 atggactgcg gattttcggt tgctatctgt accacaccaa gcaaacgagg aaaaaacact
    11101 ttaccgtcga aaccaagggc ggcctggttg agattcatct ggacctcaac ggtcatgggt
    11161 tcgtgaatgg ggccaccgtt gacatgggac gagcatcgtt tcaacccatc tccctcccct
    11221 gcaccttacg ggtgcctgag ttgattcagc agccggtcaa ggctgcaggg caatctcttc
    11281 ggtttacggg cgtcagcgtc ggaaatccgc attgcgtggt gtataagaaa caggaagaac
    11341 aatggacccg ccaggacctt cttgagatcg gccctgaact ggaaaatcat attattttcc
    11401 cgaaacgaac caatgtccaa cttgccatac ccaccgggcc gcgagccatt tctatcttga
    11461 tttgggaacg gggtgccggg gaaacacaag cctccggatc atcggcctgt gcggcagcct
    11521 ctgcaggagt ccgattgggc ttagtcaaaa gccccgtacg ggtgaaagcc caggggggta
    11581 ctctagatat taccgttgat tctcaataca acattacgat gaaaggccct gtgaccgaag
    11641 tggccagagg ggaaattagt caatcattta taaatggact gcgctaaacc acgtgttagc
    11701 tcctccccac ccactaggcc gttctctgag tttccctcac ccgttgaatg atctctcaag
    11761 ctctcgcgga taggcgaggc cattccattt gaagggccag aatcactctt ccttccagca
    11821 aggcatggtg ttccccgact ttccctctcg aatattttca aaccatcttc ttctgttttt
    11881 tttctagaat atctcggaga agaggtcggc gggcatgggc gtcagacttt ccgccaccaa
    11941 acgattaccc agacctttcg ccccgtgaca attcaaacaa taggtctccc tgctccccaa
    12001 tgtctgtggg atcacctgcc aacacctttc ccacaggcag cttgataaac acgatcacga
    12061 taccacctta gaaacgaata tccaccgatt tttcttttaa tccgctttac tctggtaacg
    12121 ttggaaactc aaacggaatc ggttcgccgg tcaatgcctt catgaaagca ataaggtcgg
    12181 ttttctcctg cggtgtcaat cccaggggtt tcatcaacgg actcaactga ggattcgcct
    12241 tcccaccttc atcgaaaaag tcgacaactt cctctaatgt tttaaaagcg ccgtcatgca
    12301 tatacggggc ggattcagta atgcttcgaa gcgttggagt tttgaatgcg cctttatcgc
    12361 gttctcgaag agtcacataa taccggccca aatcttcttt catcggtccg acttgtggca
    12421 cgccgaggtt gtgaaactga ttatccgtaa aattagaacc gttatgacac agaatgcacc
    12481 ggccttttcc tttaaacacg tccatcccac gtttggcgtc ttcgcccatc gctttgggat
    12541 cacctaaaac aaatttatca aaagtcgaat cggtggaaat gatggtgcgt tcataggcgg
    12601 ctatcgcttc cgcgattccc tgcaaactga caccggttcc aaatactttc tggaactctt
    12661 cctcatagcc ttttattttc gcaattttag gaacgacact ttcatgggtc tcggccatct
    12721 caacgggatt atgaatagga ccaatggcct gttcttccag agagccggct cgaccgtccc
    12781 aaaattggaa gggattaaat gcggtattgt agacagtcgg cgattgccgg cctccccgca
    12841 gtcctccaac tcccgccgaa gtttgattgg gatcggcaaa tcccgtgaca gggttatgac
    12901 agaaagcaca ggagatcgca ttgtttttgg ataaccgtcc atcaaaatac agttgtttgc
    12961 ccaaggaaat ttttgcggca taattcagat ttgtcggtgg tgtggggacc accgtcggaa
    13021 gggggccaat atccggaacc acatgaccat ccactgtgag cattcccttt gaggaatcaa
    13081 atccaggctc actccccatc gcgagtgagg caatcatgaa cagtcccatc atcccgcaaa
    13141 cacctgcgaa aactttcccc ttcctacctg tcctcattgg ttcatttcct cctgatttaa
    13201 ttggtcacat tcacagcaat ttgttcagtc aatagcatcc cggacatctg acacctgaga
    13261 gcaatttttt tcaataattc agtgtacctc gaatgttcgc aatatggtaa gaccatgcat
    13321 ctaaatgtga aagagatgct cccccttggt cttttcaggt aagttccttg tcaacatgag
    13381 agcgttgcga cttattcaga aaccatgggt ccatattccc accttcgcat aatcctccat
    13441 gaagcgccca tcctatccta ttgatgccgt cgttccccaa ttacgacaga cgctgaacca
    13501 acagcccatc gtccttctca ccgcacagcc gggcgccgga aaaacgacac agatcccgct
    13561 ggctcttctc caggaaccgt ggctgactcc caaaaccatc atcatgctgg aaccccgacg
    13621 gttggctgcg cgcgcagcgg cacgccgcat gtctgatctc ctgggtgaag ccgttggcac
    13681 cactgtcggg taccgaaccc ggttggatac caaaattagc ccgaatacca aactggaagt
    13741 ggtgaccgaa ggcattctca caagaatact tcaacacgat ccatcattag agagctatgg
    13801 cctggtcatc tttgatgaat tccatgaacg cagtctgcaa gccgatctgg ggctggccct
    13861 atgtctggaa tcccggaagg tctttcgaga agaccttcga ctcttgatca tgtccgccac
    13921 actggacatt gcagccattt cgcaacaatt aaaacaggct ccggtggtca cctgcgaggg
    13981 aaccatgttt cccgttgaaa cccgctacgt cggaaaaccg gatgtcaaaa attttgccat
    14041 tcaagtggca cagaccattc atcgcctgct gaagaccgaa ccgggcaacc ttttggtctt
    14101 tcttccggga gccggagaaa tccgacgaat agagcgaatc ttggcggacc ttccgcttgg
    14161 ctcacacatc cttatcgctc cgctctatgg tgatttgtcc gcccaagccc aggaccaggc
    14221 cattcttccg ccgcctcccg gatggcgcaa agtggtatta gccaccaaca ttgccgaatc
    14281 cagtctgacc attgaaggca ttcgcctggt catcgatacc ggactcatgc gcatcccacg
    14341 gtttgattca cgcagcggta tgagccggct gacaaccctc acggtctcac aacaatctgc
    14401 cgaacagcgt cgcgggcggg caggacgtct ggaacccggt ctgtgtatgc ggctctggtc
    14461 tgaggctgaa cagcgtacct tcaccccacg caccgcaccg gaaattctgg atgcagacct
    14521 gacctctctt gcgttggaat tggcacaatg gggcattcag gatccacagg aattgctctg
    14581 gctggattct cctccttcgg gggccatggc tcaggcacga caacttcttc actcccttgg
    14641 cgcattcgat gcccaaggcc acattaccga tcatggccgg tctatggccg agctccccat
    14701 gcacccgcgg cttgcgcata tggtcctgaa ggggaaggag ctggactcag gagccttggc
    14761 ctgtgatctc gccgcatcct taagtgaacg ggacctgttc aagggatcca cggcacgaga
    14821 gcatgccgat cttcgcgcca ggtttgatgt gctttatggt agcgcccatg cccagaggga
    14881 taccggattc tttaataggg gaaccctcca acgtattcgc caagtctctc aggcatggca
    14941 acgaacactt cacatcacga ctccccggca tgtccccaag cagcagattg atcagatggg
    15001 tgtactcctg gcgctggcct accccgaccg tattgcgcaa cgacaatccg atgaggccag
    15061 acgataccgc ctagccaatg gacgaagcgc gcgattccat caccccgatc cattggagca
    15121 tgaagaatgg ctcgttatcg cagaccttaa tggagcgcca gcgacggcac tcatctatat
    15181 ggctgtcccg atttcacgtg aagatctgat tactcattgc ggggatctca tacagtcaac
    15241 ggattctgta atgtgggatg gatcaacgca aacggtcagg tccatccggc aacgacgatt
    15301 gggagaactc attctggagg aaagccgcct tcccgatccc gatcctgatt tggtgttgac
    15361 cgtccttctt gaaggccttc gaaacacggg cctcccctgt ttgccctgga cccccaccct
    15421 cagaaactgg caggcgcgcg tccaatttct gcgacgcgtc acggaaccgg gatctacctg
    15481 gccggacgtc tcggatgaca cacttcttca cacattggac cagtggcttg gcccctatct
    15541 caccaatctc tcaagtctta atcagctgaa acggatcgat ctggcctggc cgcttcaagc
    15601 actcctctcc ccagaacaac ggcaaacact cgacaccctg gcgcccaccc atctcaccgt
    15661 gccgacagga tcccacattc ccttggatta tctgtcaggt gagattcccg ttctggctgt
    15721 ccgactccag gaaatattcg gacaatgcga cacgccccgc ctggtcaacg gcaaagtccc
    15781 cgttctcatt catctcctct ctccggccag acgccccgtt caggtcaccc aggacctcac
    15841 gagtttttgg gccaccggtt atcaagcggt taaaaaggaa ttgaagggcc gctaccccaa
    15901 acacttctgg cccgatgacc ctctccaagc cccgcctact cgcggcatca aaaagcgata
    15961 aacaggtgat gaataattac cggccacatg gttcccatga ggcttttcat ttgaatgaga
    16021 cctttcatgc aaccatcgaa ccgaggtcca tggttatatc cccccacaag ggattggctt
    16081 caggcctacc ttccaagctt atttgaatgc attgagattc caattgtagt ccaagtacct
    16141 caatctgaat gtttcgtttt ttaaatacgc agaaacccgt tcagggacat acatggctaa
    16201 aaacggacgc acgcctccct tggactcaaa atcctcagca aaccatttaa agatggagga
    16261 gagatacagg cgatgattgg attcctccac ctgcaatcct ttgttcggat tcgctaaaaa
    16321 tgccttcatc tggtcatcca gttgctcatg caatcgctct gacgtatagg cttccggccg
    16381 tatgtcaggg caactcaagg aggcgcaaac gatcgccaca tggatccggg gttcccccat
    16441 tttgcgtaat atgtcatgtt caatttcatt gagtgttcgt gccttcccgc gaaccgttcc
    16501 cacgttgagt ttccaaaccg aagaaaataa tcctccggca tctttgatgc ttttgagtgg
    16561 ataatgatcc aacaccattt tgacggccat gatgttgtag gcgttgatcc aaaaagccaa
    16621 cttctcttcc tgtgtgctga agccctccac tggtgccttc tccagatcgg caatgactct
    16681 tctgtatagc ggatcatgac caagtgcctg gtaattcacg gcatacagcg ttatcccttc
    16741 gatagtcttg gaggccacat actttttgag taaggcatcc caagttgaaa attcattcgc
    16801 ctgaaccgtc tgtccaaatc ccaaaaatcc acttattaaa aacacgctta ccaaataccg
    16861 tgtcattcgt atgcccagaa caaggtgctt catgcatgcc tccgtatgtg aaaactttct
    16921 actcctgagg tcccgaaccg ccatttggaa tagagtcata tgagttcagc gttcattaca
    16981 accctgacca aaccacatca acgcttcgaa tatcttcctt ggtaccttcc aggggattgt
    17041 cctgcctctc ttgcttttat tacacagaat ttttaaggtc atcctgattg atcaatgctg
    17101 acacaggtct tttcattctc gacctaggga gccggcctca tgaatgcaaa tttgccttca
    17161 caaatcattg tggtggccgg ttgggtcctg ggcagctttt ttctgggctg ttccagcgtc
    17221 gaactgacaa aggttcccaa aaaacccctg ccctaccatc cccctccact ggttgaatta
    17281 ccattggaat taccaccaga aggcggtccg cgacgcccag tcgatgccat gtctcctacg
    17341 accctatccg ctcccggacc ggagattttc attgtcgatt ccaaccaacc tggcttacgt
    17401 gccacagcag aacgggatcc ggacgtgaag acccaattgg gcgagcgatt tgcctacatc
    17461 aataccgaga aagtccctgc cgaccaatgc ctggtaatcc tacagggaga cgactcaagt
    17521 aactctcgaa cgaccgaacg ggcatcgaat tccgatgcct ccgcctatcg gttaacctac
    17581 tacagttaca cccacaatgt ggccgtgcat gtctgcatgg aaaacgaccg aatgtcttct
    17641 gtccagcagg atccgcgtga aggctaccaa cccgaagaag gtgaagaaga aatcaccgcc
    17701 gctattgaac tggcacgagc agatcaacga attgcccaag agattcagca tctgcacgga
    17761 catgccattc tcacctcacc tgaagaatac cggtatttct gggtgagcga tgaagcggga
    17821 tttggggacc gcgtgttgtg ggtcacattt tctgaaatgc cggagagttc ggcactctac
    17881 tttgcccgag ttgacctcac ttctcagacc gtcttggacg caggcaagga ggcaggaccc
    17941 caatgactat tcccaaacaa gaagtcggtc gcatggcatc atttcgatat ttcatcgtcg
    18001 gtgtcgcatt gaccagtgtg ttggctgtca gccccgcctt ggctgactgg gaatccaaac
    18061 cgtttacgtg gggaccgtgg gacctacatc ttgagatgaa ggatgctgct ggcctcgggc
    18121 tacgcaatgt gtcttatgaa ggcaaacaga ttttaggtaa agccgatttg ccggtgatcc
    18181 gggtcaagta tgttcgggag tggccggcct ggcatccgtt tagttggttt gggcttgggc
    18241 ggtccagtgg ccgatgcgga ccattcgaag accggatcag cccaccgcgc cttaaaaaga
    18301 atcctaactg cgatggcaaa cagctgtgtc aggccgaaca taccatccaa ggggtcaagt
    18361 ggttggaatt aggtgtctat gccaaaattg gtcaatacca tctgtatcaa gcctggtatt
    18421 tgagcgaaga cggtcgttta tatcccagtg ttcagagctg gaatctctcc tgtaataccg
    18481 accatgatca ccatgcctac tggcgcctgg attttgatat tggcggatcc gatcaggatc
    18541 aggtctttgt cctggaccgg aattctacaa acgacaacgg ttgggggccg gggtgggaaa
    18601 aatacctggt tgaggaggat gccaagaaac ccgccagcaa tagccaggat cgcgtctggt
    18661 ttgtgcggga ttatcccacc ggacagggtg tctggattat tccaggtcct tttgatggcc
    18721 attcgaataa tttttcaaat cgtgatgtct cgattcgaaa attctaccgg gatgaagata
    18781 ccggctggcc attcggtgca cgtggggatt tggaatccaa aagtgatgaa agcgtccaag
    18841 agaccaatgt ggtcttttgg tatgtcgccc atcttcctca taaggcagct gaaggggacc
    18901 gacccatgcg ctattggatg gggccacttc tccagattta tcaggaatct ccctaaaact
    18961 actcaggaca gcggtaatca atacccgaaa cttaaaaaaa gccgcaattc tcacggcccc
    19021 cgctcaacaa tttcttttct gagtacccca aaaaagacta ttgcctcaat ctgttagtcc
    19081 tcgtgcagtg cctcatcgcc tttttccgga aaggtttgtt atggtgaacc acccatgcaa
    19141 atcagcctac atcccctaga tcggcctttt ttctttaatt ttctatcttc accattaagg
    19201 aaccttcagt catgaaacta ctttcccgca tcctctgcat acgcgcctgc tcactcctcg
    19261 cctattcttc catccttctt tttggacctc tagggttggc agagcccgga attgatggct
    19321 tccgcgattt aaaatttggc atgacggaac aagacgtatc cgctttacct gcctgcaaaa
    19381 gttcgacgga atgcctctat gaattgaccg ataaaaatcg atatttggag ttaacctact
    19441 tacccgacac agttccaggc acacccgaac cggtccggaa actggcaaaa attaccatcg
    19501 atatggggca attcacggag gactggttcg agcagttaca aataatctta ggagatagtt
    19561 atcacctcac acaggacctc accgaaccga ccaagcaatc gtttcttgct ggaaaacaaa
    19621 acgagctgaa tttggggttt gaagagggac aggtattatt gaaggtcgta cgccgtccgt
    19681 tcgggaacct ggcgttgaag gttgtgtatc aaaatgcggc ccttgcgaaa ggattcattc
    19741 aacagagcca agttgcggcg actgcctcgt ctaactgaac ttcctgccag gagtccaagt
    19801 gccacatatc attgcgaatg aggcgattcc cctctccctc agccttcttc gtgttgagga
    19861 ttaggaagct tgtttgggca cagaagcaat tgtaggcacc agcaagcgag gtcggagact
    19921 ttcctctttc acaaagaagg aaatgggcag tgtcaccacc cccgtttttc gttgttgatg
    19981 catcaacttc cgccaacagc gccgacacag atcatgatcc tttaacgaaa ccgcacgtcc
    20041 taatttactg gaatggggat tgcgtctggg agaaacaaag actttgacct cactgccaca
    20101 tttggcacaa caggttatac cgatcccact gcctctcgac gcccggtggg tcaccagccc
    20161 aacactttcg gctatatatt ttcgttttgg attccatgct tgatcttgtg tcatcacgga
    20221 ttcaggtata tcccatgtga gcattcgtct catggccccc tccttggaaa gattggtagg
    20281 aagattcgta tctattacca ggagcaaggg atatgccggg ggccaaactg cataagatag
    20341 agaagagaaa accttatgaa aacaaaagat aagagattca agatccggaa ggctggactc
    20401 aggaagctag gcagattgcg cggtgaacgg gcatttcttt cttatttgat gtcactgaaa
    20461 agttgacacc tgttggaacc tttgacagac agaatgggag cgggaataat ccaaatctca
    20521 tgaaaaggtt gttcaatata cgcgaccagg aatgactgca ccatccctcg gatcctcgca
    20581 aagcaaccag acgcccccca accaggttag taaccctatg gacctcacat gtatcacacc
    20641 ctttccggtg ccgattcatt taaaatcagc caatagagcc caaggttttg aaccgcctga
    20701 gagaactctt gaaagtccac tggcttacga acataactat tggcaccaag ttgatagctt
    20761 ttcacaatgt cctgctcttc cttggacgac gtgagcacaa ccaccgggag aaactttgtc
    20821 cgatcatcag ctcgcaaccg tcgaagcact tccagcccgt caatcttggg aagcttgagg
    20881 tccaacagga ttatttgagg catgttgctt aaatcccgac cggcatgagc tccggttcca
    20941 aataaatatt ccaacgcctc tacaccatca cgggccacca cgacttcatt ttttatattg
    21001 tttttcttga gcgcccgcat cgtcagctct tcatcatcgg gatgatcttc tactaacaaa
    21061 attgctttgt tcgtcatcgt ctcctccttc gtttccctta caacgtaaaa tgaaaggttg
    21121 cgcccttgcc tacgactccc tctgcccaaa tctgaccgcc atgccgttga attatccgat
    21181 gcaccgtcgc cagtcccaca cccactccag gatattcagt ataggcatga agccgttgaa
    21241 acgccccgaa gagtttatgc acataggtca tatcaaaacc cgccccattg tcggtgacga
    21301 agtaggcggc ctgccctttg tattgcccat aaccgaattc gatacgaggg tgagcttgtt
    21361 gctgggtaaa cttccacgcg ttacctaaca aattttctag aaccgcccgg agtaattgcg
    21421 gatcagcggt ggcgaataca tcattggcca caatacattc tacctgccgg tccggctcca
    21481 ccttctgcaa gtcgtcaaga atcccccgaa ctacggcgct catatcaaag gtctgtgtat
    21541 gaatttcagc ccgagtcaat ctggcaagat tgagcatggc atcgataagc tgagacattc
    21601 gttgactggc agtccgcacc ctacgcaaat aacttcgccc tgactcatca agcttttcat
    21661 catagtcttc cagtaccgcc tggctaaacc catcgatgcc ccgcaagggg gcccgcaagt
    21721 catgcgagac cgaataacta aatgcctcaa gttctttatt actcgcctca agttgcgccg
    21781 aacgctgaac caactgggaa ttgagtttgc gtatgtcctc ctcggcccgc ttccgctctg
    21841 ccacctcccg aatgagttcc gcatttgagg cagccaactt tcccgttcgc tcctggactc
    21901 gcttttccag attatctttc gcctgccgaa gggtatcttc ggccaattgc cgatcatgaa
    21961 tctctaattc caaatgtttg ttgacacttt gcagcgcgcc ggttcggtct cgaacctcct
    22021 cttccaattc gcttgctacc cgtccgagct ttaagacgac taaccagaat cctcccatcc
    22081 aaatgacgac caacatcccc atcaatccat aagttccgag ccaactcatg cccatgattg
    22141 tctctaccga attaatggga tagacaacct ccaacaagcc ccccacttgc ccgatctgcc
    22201 aatcacgctt cggactatcc ggatgtctgt tatgacactc cacacattcc ttctgcattc
    22261 gatcggcttt cgcaaaacgg agggactcac gcccatcgac ttgttcaaac cgatagaatg
    22321 gctcatcaga atcaggatgt tgcgcgaaaa attcccaggc ctctccctgg aagctatcca
    22381 taagtccccc tgatttcact ctattttgaa acggataggg gctcattaac ttcaattgtt
    22441 cgcctggtcg cccggccacc aatcgacgac ccagatcccg gcttagagtc gcagggagag
    22501 ggatttcgcc ctgatggtct cgataccctt ccgaggcttc gattccctgg gcatgaagac
    22561 gttgaacaac ctcgacggtg taccatatcc gcatctcatt caacgctctg gtatagatct
    22621 cggatccccg aaaggccatg accgtcacca tttgagattt caattgagaa atatgcaaat
    22681 aaatcacgat aaggcaaagt ccaaagagca cggataacag caaaatcgta tgctggtgta
    22741 agagctccat ggctcccatg atcctgttca cacagatgcg ccacagtctc aaaaccgaca
    22801 atggcaattc tttacgaggc gtcaactcag atacagacaa aggtctttcc tattctcaat
    22861 tcaggctttt caaacccctc cctcttcgag ggcggtcctc tgccttacgc tatcggatca
    22921 ttaccaacta aattgagcct cgttccaatt tgagccctcc ctcaacccat tgccccgata
    22981 atgttgcctt gcaatttctt ccaatgaatt gcgcttgaat cggccgccat atgtagaaca
    23041 aactgttcaa atcattaagg gtacacatcc gatcattgat atgattccgt accttcaatg
    23101 tgagagcgga aaggagggga agcacatcag aagccaccga tgggtctcat ctgggctcct
    23161 gtatcatgac cgttctccac caataacctg ggactgccat cctattgttc ccggtgaaaa
    23221 tttggaattt gcaccgccag tcttgactgg cacgtcaact aatcagacct gattacgtct
    23281 ggaagggatg gaggggaaga agggaatccc aattgggtaa aattgctcac gaggtattgc
    23341 actgggagct aacatccatc gcaaagaatg ccaccgaagc agaagccagc cgattggaag
    23401 acaacccgac cctcacgcta ctaaattaag ccactcattc cccgacgata ggatggccgc
    23461 tgcgcatttc ggtcgaccct tcccggctgg cccccttact cacacgggag cttgattttg
    23521 taaggcgact gccatcaagc attcttcaga ctctgatatc tttccagggt cagtgtcatc
    23581 acccatactg ggctccagtt cctgatcctc gtcatacgtt tgccgcacat caagaatttc
    23641 aatgtccaac tgggcatcag ggtacaatcg tcgcaagtcc cattcggtaa tccaccctcg
    23701 cacatactcg cgcatccggg tgctggataa tttttctaca gcggaaacgg gtggcggtgt
    23761 ccagctatag gcggacccat gaagcgtccg aagccgctca tataacgcat ctaccattga
    23821 ttcataggga cgaactaatg gttcgccctc ctgttcaata agccggatac cctggaccgc
    23881 ctgcaaggct tgcatatgag cttcatccgt cgattcctcc tgacacatct gctcaatttt
    23941 ctgagaatcc tgtttttctt ccaagaccac actttgaaaa tcatcggtca aagccacaat
    24001 ggccccaata gccggacagg aatgatcggc tagcaccccc atccaccgcg ccacctccat
    24061 ataagactta gtccgttgct taaaggaata ccgaccaatg atctccgctt catcaattaa
    24121 cagtatccac cctttatagc cggcagcgac catcaagcgt gaaacaaatt gaaaccggcc
    24181 caaggccaaa tcctgactgg aaattttatt aaacacatac gggttttccg gcgggcaccc
    24241 ctggaggtat ttattaagtt ggctattact tataggatct cctgcccaat accggatcat
    24301 acgatgactc agctccgggt cattgatcat tcgctcatag agatacaggc tggcagcaaa
    24361 ccgggaatcg cagacctggt ccttccgatg cacccaatca aataaatcgg catactgcgg
    24421 gctttggaaa ttcagttcac cggctatttc ggttagagca tcgccatgtt ttccgggaac
    24481 aatggcatta ttgatggctg cacgaaagaa gggcactcca ttgtacaatg gagtctcctt
    24541 actgattaca atgggactac agacaaagtt ctgctccagc gccacctgtt gcaacgcatg
    24601 aagaagatgg gactttccac tcccaaaccc tccttcaatc acgataccct ttgtaggcat
    24661 ttcatgagga acgtttgcct ccatctgttg aatcagccgt ctgaattttc cctccaggtc
    24721 cggttggtgg cttccaagta cctgcaccac atcacgattc ggcaccccgg atcgcaaggc
    24781 ttccatggcg cgttgatgtc caacatcacc attggccatg gcaggccggt cattgtattc
    24841 cctctttccg gtatcgtggg cgtccccggg ctgttgagat ccaagacgaa tcaaagcccc
    24901 taatgggttt tggtattgac gatcacggac ttcgtcccaa atacgcaact tcagcgcctc
    24961 ataccgattg agcccccgac gctcatctgt caaaaattct tgcggtacgt ggaccaccag
    25021 cagaaaactt tccaattcat ccgtcccatc aataaactgg cgaagcattt cgtaggcatc
    25081 taacgtggcc gagacgccat aatacaagcc ttcaggatcg tcaggtttct tactgagcaa
    25141 acatcgactc aagtccaggg acacgactaa ccctgttatc cctactaacc gtaaccaacg
    25201 gcataacgaa acaaacatat atcgagcatt cgtccggtta attttctgaa aaataagggc
    25261 ttctttgaga gctgaaatct gacgaatctc cccgcccagc caggacttaa ccggttcggt
    25321 cataaagggg acttccgagc cagagtccat ttggcccaaa cacaatcgga tcatggccat
    25381 acgaaattcc tggcacatct gcgtatctcg atagatggcc cgctctaacc aactatttaa
    25441 atctcgtcgc aataaaggtt ccgcccgttc attcatccgg gcgactccag ccatgcaaaa
    25501 ctcttcttta ttctccggga tttggtaccc attttcttga agaagtcgac gaacgaattg
    25561 tgaagccagg tcatcccatg gaattcgacg agcaatttga tgaaagaatt tatccatcat
    25621 atgaattttg gtgtctttgg catcaaccga aatgcacgcg tacccttcct gctgagacaa
    25681 gaccgccaat tgattttgga cgtccactct atccgggtca gagtctgtca ccacgaactt
    25741 gaccgatgag cctccttgcc caataaatcg ttgcagatat tcttgccgaa gcgtataaaa
    25801 ccattcttgg ggccgtaact ccattggttg tatccttcat aggttaaaaa gtcacatgca
    25861 aacgcgggtt ggtttttaca gggtgacgaa aagaccggga tctatgctga agaaacgtgt
    25921 actccgcccg cgatagtttc gatttgaata ttttgctcgc catattcaaa aatcctgatg
    25981 acggcgattt tggtcaaacc ccggattaaa tggattcgga gagtgccggg tgaagaatac
    26041 cttcctgatc accttctaat tcacgatctt ctgaataatc ttgtcgaatt tccgttagtt
    26101 caatttcgac tttttgtgtt ggatcaagac gcgttaaatc ccattcggta atccaacctt
    26161 tgacatattc acgcatccgg gtgctggata accgctcaat cgtggggacc tgaggtggtt
    26221 cccacccata cgccgcacca tgaatggaac gaattttatg gtagatttcc tccaccagct
    26281 gatcataggg accaaccaag ggtatgcgct cgcattcgat gagtcgcatg ccctgttccg
    26341 cttggcggga cagcactaaa tcggtttctg atcctgtttc acgcagtttt tctggtactt
    26401 tttcccgatc acctttttca tccagaatcg cactttgaaa atcatcggtt agcgccatca
    26461 ccgccgccaa tccggattta tagccaagat ccgcaaagct ggaagcttca agtctaccca
    26521 gccacctggc taattccgca taggattggg ctcgctgctt aaaggagtac cgtccaataa
    26581 tttccgcttc gtcgatcagt aaaatccaac cagaatatcc agccgcaatc atgagtcgtg
    26641 aaacaaattt aaaccgttgc aacgccatct ccattggcga gacccgttca aatcgataag
    26701 agacattggc atcgcaggat ttgagatatc gcttgatttc agcatcggtc aaggggtccc
    26761 ctgcccaaaa ccgaatgatc cgatgactga gttccgggtc atttaccatg cgttcgtaca
    26821 aaaataccgt ggcagcaaag cgcccatcaa tttccccgcc ttgacgatgc acccattcgt
    26881 aaaaatttgc atactgagga ctcttgaaat tcaaatctgc tgccacctcc gccaagacat
    26941 ctcctcgctt ttctgggatt tcagcagact caatggcaga tcgaaacatc aatgcgggac
    27001 tatacagagg cgttt
//
LOCUS       DAORAQ010000021        26944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_348_length_26944_cov_68.488341, whole genome shotgun sequence.
ACCESSION   DAORAQ010000021 DAORAQ010000000
VERSION     DAORAQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 26944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26944
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_348_length_26944_cov_68.488341"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKM72_03605"
     CDS             complement(<1..406)
                     /locus_tag="PKM72_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59899.1"
                     /translation="MTGNIRYLFNGLIVGIFGMLCALAPTEAALLDGFMGGVEESLGK
                     KAEEKGERLVIGNFYQAERPDSHAPVGVMQDHTHNKGEFMFTYRYMYMYMDKMRNGTD
                     NLSNNDVLKDFAVTPKNMTTQMHMFSAMYGLND"
     gene            complement(622..921)
                     /locus_tag="PKM72_03610"
     CDS             complement(622..921)
                     /locus_tag="PKM72_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59900.1"
                     /translation="MKRISPLFVACLVFCLMVLSAVLTAQAVEHSQHHSQHHQGTHST
                     FLCTWFCAAGQTLETNRVLVDGPVKSLLRIEDWRPIAPQVILVIIPASRAPPPCL"
     gene            complement(1265..1660)
                     /locus_tag="PKM72_03615"
     CDS             complement(1265..1660)
                     /locus_tag="PKM72_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP59901.1"
                     /translation="MIVQIMQKKLKTIPEGSSITHAAEKMQTEGIGSLLVERKGIPVG
                     IITDTAIVHKAVGLKKNLDATKVESLMTSPLPIIQLAQTSHDALDLMGDLGVRHLVVC
                     DGPEIVGLISARDLLVYFGSVSEPRIGVD"
     gene            3016..3399
                     /locus_tag="PKM72_03620"
     CDS             3016..3399
                     /locus_tag="PKM72_03620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNP59902.1"
                     /translation="MSGSDLLLEYLTKYLPILIFVGLAMGFGLVTLVLSYLVQPKYPE
                     PEKLSTYECGSEPFSDSRMPFPIRYYVIAMLFVIFDIEVIFLYPWAITFNQLGVIGFI
                     EMMIFIGLFVVAYVYAWRKGALEWD"
     gene            3390..3905
                     /locus_tag="PKM72_03625"
     CDS             3390..3905
                     /locus_tag="PKM72_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNP59903.1"
                     /translation="MGLIQIGKPPKDGELGVMTLSLEKVVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDIDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VISMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQDRIMQKR
                     VFTKQPEQVKV"
     gene            3925..4419
                     /locus_tag="PKM72_03630"
     CDS             3925..4419
                     /locus_tag="PKM72_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNP59904.1"
                     /translation="MHPIAEKIQSRFPEGFVGANEWRGDLTVTVRREALHDVCSYIRK
                     DPDLDYDYMVHVSSVDWPDDEERFELVYEVYSIRKRHRIRIKTRVPEHDCLVDSMTDL
                     WMGADFMEREVFDMMGIRFNHHPDLRRILMPDDYTEGYPLRKDFPVQGKGWRDTFDFL
                     NDPT"
     gene            4468..5712
                     /gene="nuoD"
                     /locus_tag="PKM72_03635"
     CDS             4468..5712
                     /gene="nuoD"
                     /locus_tag="PKM72_03635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNP59905.1"
                     /translation="MKLEDQRTTVYKVDPNNPETESLPTLRTEELLLNMGPQHPSTHG
                     VLKVILELEGERIVKSTPVLGFLHRGVEKLAEDVTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLMDITVPDRAEYLRTLVAEVQRIIGHLFWLGTQALDIGAMTVFFYTFRDREI
                     LLDWFDELCGARLTTSWYRIGGVERDFTPSLIDKVQKFLDYFPPKIEEYVVFLEKNRI
                     WLARTRGVAVISAEDALSFGLSGPTLRGSGVDYDLRKYEPYSAYPKCEFSVPVGKNGD
                     TYDRYWIRIEEMRESVKIVQQCLNQMEPGPIMADVPSVTLPPKDRVFTNLESMIQQFK
                     LFSQGFDAPAGEIYCGTEAHKGELGFYIVSTGGGRPYRLKIRAPSFIHMGAFDYMAKG
                     YMIADAVTIFGTYDIVMGECDR"
     gene            5774..8464
                     /locus_tag="PKM72_03640"
     CDS             5774..8464
                     /locus_tag="PKM72_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNP59906.1"
                     /translation="MGLKPVTTPDVEAATIELTLDGEVVQAKEGVSLYDVISSTGKIV
                     PAMCYHYTFDPFGSCGMCLVLQEGKKAPVRSCTAKAAAGMVIQTEGEDLFLARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKNIPEDTRSLVLDFNMNRCI
                     ACGECINICKDVLRIDALQFMKKGGFTQVVAKGDQALDCEFCGDCLAVCPVGAITNKF
                     SKYAYKPWQLKKTTTTCNYCGDGCQIYVETKDTEVVRVTSPLSWKNKWGDRTDTINGH
                     DGICVRGRFGFQFIDSATRLKTPLVRTESGLQPSPWLDALDMASGRLAHVKAQYGAQA
                     IAGLITARCTNEDLYVFQKFMRSVIGTNHLDSSARYGHLNFVRAIKHALGLHRMMNTS
                     EEINKAKAVLIIGANITETNPVASLRVKSAMGLYKAQTIVVDSMQTNIAKLASHPMMV
                     KPGTEGLFIQGLVKSVMDQDLIDEEATSAFPEALNSLRQAVGVLSLEDVANQTGVNPD
                     QIHEAARIFAEASRSIIICGEGILRHAGGYQHMLNLIDLAWVTGKLGRKGCGINTYTE
                     EANEQGAVDMGVAPEFLPGPVSFTDSQARETFARIWGNNLPDVDRGANLTEILDRCQN
                     GEIKALYLVGENPLETLPASYDVTSALAKLEVLICQDPFMTEIAKMAHVVFPASTFAE
                     KDGTVTNQEGKVQFLRPALDSLGESTLDWHIMVGMANGLGSPMEYETTQDIQNEIRKV
                     LPGYYNLGKVAHEAPDLSGYFSKAFPEEVASRYSVVRKSPTQRTFALRMIQLIYHSGK
                     LSTRASGLMEISPNSSRLRMSAQDFNDLGLSAHDRVRITSEQGEVEMGVEVDMSMMSG
                     TCAVPEHFNDPPVKDLMSHQVDPVTGVPYFKLTYVSIEKA"
     gene            8488..9057
                     /gene="nuoI"
                     /locus_tag="PKM72_03645"
     CDS             8488..9057
                     /gene="nuoI"
                     /locus_tag="PKM72_03645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNP59907.1"
                     /translation="MSRSSETKKFFDAVLFKEIWTAMKVTFKHMLHRPITFQYPREKR
                     TIPDAHRGALCLLRYEDATERCVGCDLCEAACPSRCIKVISEEDKTLPLQRYASEFYI
                     DITKCVFCGYCVEACPVNALAMTKVYEFSTHDKRTLLFDKARLYQIGERHIDDAKKYL
                     YAHNQEKEGEEGRAYRYHFPVSIKSTQEP"
     gene            9065..9607
                     /locus_tag="PKM72_03650"
     CDS             9065..9607
                     /locus_tag="PKM72_03650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNP59908.1"
                     /translation="MMWLLFGYFATVSVVAGVLTVALRNAVHCAMALLTLLLHVAGLF
                     VLLNAEFLFAVQIIVYAGAILILYLFVLMLMNLKTEDQHLHKKYALLLFAGMAIFGEL
                     LILLVQSPYGGVIGTATPDLILQTGPSHAIGITMFSDYLLLFEVIGIFLLGAVIGAIV
                     LAKTPAKTDPQEEPVISSMP"
     gene            9620..9922
                     /gene="nuoK"
                     /locus_tag="PKM72_03655"
     CDS             9620..9922
                     /gene="nuoK"
                     /locus_tag="PKM72_03655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNP59909.1"
                     /translation="MIPLSAYVALSAILFMTGLVGVLIRRNFIVVLMSVEIMLNAANI
                     NLVAFSHYLHSMSGQMAALFIIAVAAAEAAVGLAIIIVVFRGKMATNVDQINILKW"
     gene            9924..11921
                     /gene="nuoL"
                     /locus_tag="PKM72_03660"
     CDS             9924..11921
                     /gene="nuoL"
                     /locus_tag="PKM72_03660"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNP59910.1"
                     /translation="MFDLLVLLIPLFPLLAVLANGLLGSRYSSDLAGRLAFGSVGLSF
                     LCVLGVFVSFLGDSTPREVIAYSWIFGGDLSINLAFLIDPLTVIMLLVVTGVGFLIHV
                     YSVGYMHGEEGFTRFFTYMNLFMVSMLLLVMGNNYVVLFIGWEGVGLCSYLLIGYYYE
                     KVSAGKAATKAFVVNRIGDAGFLLAIFLIFTHFGTLDYTAVMAQAATLSTEMATAISF
                     CLLVGAIGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMVVRNHAIFDMAPM
                     ALAAVGIVGGVTALFAATIGLVQNDIKRVLAYSTVSQLGYMFLACGVGAYTAAIFHLM
                     THAFFKALLFLSAGSVIHALGGEQDIRKMGGLHDKIPTTYRVFLIGTLAIAGIPPLAG
                     FWSKDEIMAHAFVHGYYHLYLLAAVGAFLTSFYMFRLTYLTFYGSSRVDPHVAHHIHE
                     SPSIMTVPLMVLAGLALLGGFLGVPPEHGWFHGFLHDVATPLGAEAHEVGWGMLLGLM
                     SVAIAIALGGWGLAHYMYSIRPQMANEWAGKFPRAYTTILNKYYVDEIYDFLIVEPCK
                     KLGLLWDWLDRQVLDRFVVGIGKGTDLSAAAITWTEKHVIYAGLNVVGYSNHLLSWSW
                     RKMQSGMVHHYAAIIVIGLALLIHLVLVWFVGSSAADMSIR"
     gene            11931..13484
                     /locus_tag="PKM72_03665"
     CDS             11931..13484
                     /locus_tag="PKM72_03665"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNP59911.1"
                     /translation="MQEELQFGFPILTFLTFFPLVGALVIWALKDQDLIRKATLGIAS
                     LEMVVASLMLWRFIPETAAMQFAERHEWIPFLGISYHMAVDGISVLFVGLTAFLILLL
                     VLYAWDTVKTRPKAFYMCLLGMEATVMGIFVSIDLILFFVFWELMLIPSYFLIKLWGP
                     GEQRHYAALKYVLYTLLGSVFMLVGFCLMSLNYFEAKQLYSFDLLDLLAVQIPLSQQL
                     LIFWLVFMGLAFKAPIFPFHSWLPDALVQGPIPMSVMLAGIKLGTYGFLRFSFPLLPD
                     ASQHSTVVAILMTMGLAAIVYGAIVAIIQPDFRRLLVFSSISHLGFVVVGMFALNFQG
                     IQGSLITMINLGFSTAGLFFLAGFIYERLGHNDVRRYSGLAKKMPLLATFMLIIGMAS
                     IGLPGTNGFIGEFLILLGVFQAWWLYGAIAVTGIIFAAAYFLWFYERSIFGPLKDSVP
                     AAIKDLNSREWAIGVALSVMIFWIGIYPSPFLRMVNGSVQEVVDRLHPGMATAHHRDA
                     LLSAEVTGN"
     gene            13490..15085
                     /locus_tag="PKM72_03670"
     CDS             13490..15085
                     /locus_tag="PKM72_03670"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNP59912.1"
                     /translation="MGEYSLLIILFAPFFGAIALMFVPRQEAILVRMTAAISAGISLL
                     ASFYIFLAYDTTKGGFQFVQRYVWSEELGIAFYLGVDGIGGPLVFASAILLFAGIFVS
                     WHIKDRTKEFYMFLLILAAATIGVFMSLDLFFLYFFYEMSVLPMYLLLGLWGSHTKGY
                     LAMTNPEERKLRDSIGFIFNFGSNSKEYAAMKLVLFLSAGAVVALMGILLIYHLSGLH
                     TFDIVVLREQAHFSDNVATLIWFLIFFGFASIAPIWPLHSWSPVGHAAAPAATSMLHA
                     GVLMKLGHFSIIRVAFEILPDATREWMWVAAVLCVFSIVYGGLVSYFAKDTKYVIGYS
                     SSSHMGYIFLGMAALSYISLSGAVIYMFAHALATSMLFAMAGWVYDQTHSRDIPSLGG
                     LVEKMPFIAGAFIVGCMASIGMPGTINFIAEIMIIVGSWDKYPFQVLVAVLGIVLTLA
                     YMFKMMRGLFYGKLDPEYAHASDARHWVDRMPLLLLIVCSITLGIFPGHFYEVVRSTV
                     EPMVDRINHVAPLITQSTKGFLP"
     gene            15130..16617
                     /locus_tag="PKM72_03675"
     CDS             15130..16617
                     /locus_tag="PKM72_03675"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNP59913.1"
                     /translation="MTFQLDLSVADLLLLLPEIFLTLWLSLLLAMDFSLKRLTHQQLA
                     YLSIAGMAVTGLILLGFDQAGTTGALFKNMFVLDQMAIVFKLIIVGATVLVLFMSMDS
                     VQDFKFFRGEYYVMVVMSALGMMFMVSSNDLLSVFVTLEFSTFGFYVLVAYLRDDQRS
                     SEAGLKFFILGIFAAGLLAYGISLVYGETGTLIFSEMTSTPGSYGLAIGYILIFAALG
                     FKIGAVPFHAWIPDTYQGAPTPVTAFLSIAPKAAALAILVRMFYVSLASFKPMWALLF
                     VVASIFSMTYGNIVAIAQKNIKRLLAYSGIAQIGNIMIGLAAGTKQGSDAIMFFLLTY
                     LFANLGAFAVIIAVSQVMKSDEIEDYNGLNRRSPFLAAAMLLFLLSLAGVPPLAGFIG
                     KIYLFIAAMKEELYTLLVVGLINIVISMYYYLIVVKKMYINEPTDPSPIHASMPIKVA
                     VYVGIAGTVLLGVYPGPFIDWVVGASLMFSNIAGPTAAASLLPGG"
     gene            17769..18974
                     /locus_tag="PKM72_03680"
     CDS             17769..18974
                     /locus_tag="PKM72_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP59914.1"
                     /translation="MANETEEQNHSITQTVSQRYAKAVTNGEQLCCPTGYNHEDLGQF
                     IPEPVLKVSYGCGTPVGLSTVQQGEVVLDIGSGGGIDCFEASRKVGAQGRVIGIDMTD
                     EMLALARTHAPMVAKNLGYPEPNVDFRKGFADAMPVDDDQVDLIVSNCVINLAPDKGK
                     VFQEMYRVLRPGGRFTISDIVADQPIPNYLIYDKAKWGDCLSGALTIKDYWGGLWDAG
                     FKGVHLVNHIPWRVIDGIQFLSVTLTGYKLASASTTPSSTFATLTGPFSQVVDDLGST
                     FTRGHPQQINERTAALLALAPYQDLFIIDEKPVALAVQDPRMRAVYPDEAPCVWEGQF
                     AVLTGPFLAVCDDDHHTYQCGEPIEICSKTYKVLQTDSYQPYFATINRSRDGVHSEPV
                     ICGTSEGCC"
     gene            19020..20036
                     /gene="arsS"
                     /locus_tag="PKM72_03685"
     CDS             19020..20036
                     /gene="arsS"
                     /locus_tag="PKM72_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNP59915.1"
                     /translation="MTMKTSALPMVAPVQPSWIAPSFEQALALHHSLPLKAQVISTLQ
                     VNVGKVCNQNCHHCHVDAGPQRTESMSRTTIDQILDVLDRTPQIRTVDITGGAPEMNP
                     HFEYLVEQCRARDRRVIDRCNLTVFYVKGKTHLPRFLADHRVDIVASLPCYEADNVDQ
                     QRGKGVFDRSITALQTLNGLGYGADGSGLMLDLVYNPLGPVLPPPQVELEQDFKDELG
                     QQFGIQFNRLYTITNMPISRFWDELHDAGQLEHYYTLLLNNFNPLAVEGLMCRSLLSV
                     GWDGRLYDCDFNQMLDLPIQPDSQAWIGRFNLSEWEHRSIVVGPHCLGCTAGSGSSCG
                     GALT"
     gene            complement(20080..20328)
                     /locus_tag="PKM72_03690"
     CDS             complement(20080..20328)
                     /locus_tag="PKM72_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNP59916.1"
                     /translation="MYKIIIILILLAVLFFMIRRAAREWSQPKLDPATPGKDVMIQDP
                     VCKVYVAAGTAIVQERKGKNYYFCSQDCARHFKPEDVT"
     gene            20527..21297
                     /locus_tag="PKM72_03695"
     CDS             20527..21297
                     /locus_tag="PKM72_03695"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNP59917.1"
                     /translation="MLIRAILIILAASGCTMNPPFTGTILDLTYPFNEQTVYWPTNEP
                     FHWKKTSWGPTQQGYWYASAVYSASEHGGTHMDAPIHFSESGWSLDEIPVGQLTGEAV
                     LLDIRAQVDSNPDYTLQVDDIAQWEISYGPIPPKAIVLLFTGFGQYWPDKVRYLGSST
                     PEDPTTLHFPGFSAGAVTFLLTKREILGIGIDTASIDAGQSRDFPVHQILGRANLYAL
                     ENVAQLGRLPPRGARVTALPMKIQGGSGGPVRIIATVP"
     gene            21383..21658
                     /locus_tag="PKM72_03700"
     CDS             21383..21658
                     /locus_tag="PKM72_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNP59918.1"
                     /translation="MVTITLMGDLQTVDGERALGCEIADSISVKQLIRKQGKPLREIF
                     QLLKEKKVMVTVNKRIASEETKVYDGDAVYIVAHDGMGQSGLTLSHY"
     gene            complement(21872..22279)
                     /locus_tag="PKM72_03705"
     CDS             complement(21872..22279)
                     /locus_tag="PKM72_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNP59919.1"
                     /translation="MTTQTQDIQVRDKREVKSDAEQTIPGRVFSPNVDIFEDDQALTI
                     VADMPGVPSENVSIDLRNDVLSLSGVPSVSVPEKEEHVLREYETGKYFRQFTLSEVID
                     QEHIEAKLNNGVLRVTLPKVGPAKPRKIQITEE"
     gene            complement(22340..22801)
                     /locus_tag="PKM72_03710"
     CDS             complement(22340..22801)
                     /locus_tag="PKM72_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNP59920.1"
                     /translation="MVIRRLENWPINGFSGGVSELDRLRRDLGHLFNGLSRVNGWDSP
                     AGVYPLMNVSQDSENVYVRSEVPGMTLDQLEVSVTGRNLTVTGERAIPDEQSNVRYHR
                     REREAGKIRRQLTLPTDVDSERVQAKYQHGILMVVLPKSEKAKPKKIAITG"
     gene            23407..23856
                     /locus_tag="PKM72_03715"
     CDS             23407..23856
                     /locus_tag="PKM72_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP59921.1"
                     /translation="MNQRSFGLGLLFLMMLVWSGCDSGEYGKETKPEPKAESTTMSPA
                     EAPSMAETPQVEGALMAMAGVAGAAENEEGVNHFQQGHWDVAQEHFSKAIAANADLPE
                     AHYNLALALDKLGNHGDATNHFKKALQLAPADPLIAGSQILQAHVGG"
     gene            24127..24789
                     /locus_tag="PKM72_03720"
     CDS             24127..24789
                     /locus_tag="PKM72_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59922.1"
                     /translation="MDALYYPFHLCHEKTLHQLLENYSHVHFLDFMALQLTPFMGATG
                     FPDRMGDYYPDLLESGRIIQGHNVSGPLSGAKVAAVNQDLADPLWRSIFHEALKDDRR
                     FQRGLVAFSQDSQGRSSVPTDSALLVAFQQPEREFVPYTVETLQAMSRRRLPERESLA
                     FEYGFGLIKTSAAVIYSIRRCQQLGSVAVTDSPGHHQLLTRACQRDSVELSNYCVKRE
                     GY"
     gene            complement(24973..25896)
                     /locus_tag="PKM72_03725"
     CDS             complement(24973..25896)
                     /locus_tag="PKM72_03725"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HNP59923.1"
                     /translation="MRQPCFWIIPTEHAHDRLGISEAVRKLQNCLLPAILLIIFLPLS
                     VTQAGTIPELMLADIYRQGLTLSQYRVSEKLDGVRARWDGTHLISRGGNVFAAPAWFT
                     KGFPAVPLDGELWIGRSRYEEVSSIVRTQQPHDGWRNVRLMVFDLPAHGGTFAQRVLE
                     MNRLKIEGSAPYLGIIEQQRIGSEEDLLKWLHTVTDEGGEGLMLHRETALYASGRSQD
                     LLKLKLFTDAEATVIGYRPGKGQFAGQIGSLKVRTDDGVIFFIGSGLSHEQRRRPPPL
                     QSRVTFRHQGLTENGIPRFPVFLRIRDEEPR"
     gene            complement(25924..26763)
                     /locus_tag="PKM72_03730"
     CDS             complement(25924..26763)
                     /locus_tag="PKM72_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNP59924.1"
                     /translation="MTLTAYNNVPLPSLMYGTAWKEGATTHLVQLAVGSGFTAIDTAN
                     QLIHYREDLVGEALLALDKLGVKRETLFLQTKFTPVNGQDHRTPYDASADLTTQVQQS
                     FANSLTHLHTDYVNSYILHGPYSRFGLGDSDREVWATMEDLYQSGKTKMIGISNVTAG
                     QLTQLCEEARIKPMVVQNRCYASHGWDREVRGICQTHGIIYQGFSLLTANRGVMADPD
                     IRTIAKRLGTNPAQVIFCFALQIGMLPLTGTTSQQHMEEDLQAESFSLLPEEIELIDT
                     IGE"
BASE COUNT         6269 a         6371 c         6808 g         7496 t
ORIGIN      
        1 tatcattcaa gccatacatc gcactgaaca tgtgcatctg cgtcgtcatg tttttgggag
       61 tcaccgcaaa atcttttaac acgtcattgt tggacaggtt gtcggtacca ttccgcatct
      121 tgtccatata catgtacata taccggtagg tgaacatgaa ttcacccttg ttatgggtat
      181 ggtcctgcat cactccgacc ggagcgtggg aatcgggtct ttcggcctga taaaaattcc
      241 cgatgacgag ccgttcccct ttttcttcgg ccttcttccc taaggattct tctacgcccc
      301 ccatgaaacc atccaataag gcggcttcag taggtgcaag ggcacagagc attccaaaaa
      361 ttccaacgat gagtccatta aatagatatc gaatattccc agtcatattt tttgccgatc
      421 ctttttggat taaaattttc aattaatcca gtcacaataa ataaatgatg ttgaaatatt
      481 tttaaattaa tcggtatcgg gtaacgggtt tttccaaaga gcaaatttcc gctcgcaaac
      541 aattctcgcg aacgcataca aaaataaaaa acgcgtaaca ccgaatgtgt ttgaaaccca
      601 agatatgcgt aatgataaaa actacaggca gggaggagga gctcgggaag ctggaataat
      661 aacgagaatg acttgagggg cgataggacg ccaatcttcg attcgaagaa gagacttaac
      721 cggcccatcc accagcaccc ggttggtctc tagtgtttgc cctgcagcac aaaaccaggt
      781 acacagaaat gtggaatgag tcccttgatg gtgttgggaa tggtgttgac tgtgttcaac
      841 tgcctgagcg gtgaggacgg cactcaggac cattaaacaa aaaaccagac aggcaacaaa
      901 taggggtgaa atgcgcttca ttttgagagt gcataaagtt ttaaggcctt taatttcact
      961 aacagatatg gagacctcac ttcaacccta tgaggatggg gcatttttcc acaacggccg
     1021 ttttaacaag ggtttggaag gactaaggga gggttcgagg cgttggaatt gtatcgaagg
     1081 atcaggccat tgcgggtttg cgagtcctgc acctaaaggg atactttttc aagatacggt
     1141 tgggtaacgt ctcaaagagc atgaaaagct cgtgggcaac ctcactaaaa taacctcgat
     1201 tgcccgcgag gctctcactt tatcggagta tgaaaaggaa ttttctgaaa atttatccgc
     1261 tgaattaatc caccccgatt ctgggttcag agacacttcc aaaatagacc agaagatccc
     1321 gtgcagaaat taatccaaca atttccggtc catcacatac aacgagatgg cgaacgccta
     1381 agtcacccat caaatccaac gcatcgtgcg aagtttgggc caactgaata atagggagag
     1441 gggaggtcat gagactctct actttggtcg catccaggtt tttcttgagg ccaacggcct
     1501 tatggacaat agctgtatca gtaatgattc caactggaat tcctttccgt tcaaccagca
     1561 agctcccgat tccttctgtc tgcatttttt ccgccgcgtg agtgattgaa gaaccttcag
     1621 ggatggtttt caatttcttc tgcataattt ggacaatcat gtattgttcc cttctaaatg
     1681 aagtttgtga tgttttttat tataccagtt ttccccatac cgtcaccgta ctttattcat
     1741 tccaatcaaa tgcctatcaa ggaggcagga cggggcaacc ttgcaacgaa attcaatttt
     1801 ccagacccca aaaagctccg tgactcaagg taagacgaac ctctttcgat gataatgggc
     1861 gaaatttttg gaaaagcgtt ttttaattcg aagcggcttc tttagcaatg actccatgcc
     1921 ccgtatttta ctccacaata accgactttt gaatccccaa gagtaaatgc agcttttgag
     1981 agtctccaga tgttacccta tttctatgca ttccgaactc tcacaaaaag ccaaaagtga
     2041 tccagaaggg gaatccaggc cttcacccaa aaaaaggatc caaacccaaa ttcaccgacg
     2101 tcagaaagcg aaggcagttt ctccattgga agacgagtgg aatgaccaat ctgataaccc
     2161 tcctaatctt cgaactcctt cgtcggagat gaaaaaatag tagttattcc ggtgtttgtt
     2221 ggatgatcgc caggggggct ggcttccttg accatgatgt atgtgcgtag ttcgaattca
     2281 agtcaggcgt gggatgaaaa cccgccccta aatttaggtc tgttcctcca ccgcttcaaa
     2341 ttgtccattg tcccctgatt ctatccgtgg ctttttccac taattgcgca ttggtcggtc
     2401 ataaaaacat tacttctccc atattccagt ttctgcgccc agcatactaa acaggatgaa
     2461 atttctcctc ctagatttct tgtgataatt ggagaaggag tttcgttaag aatgggaatt
     2521 tatagcaaaa aagatgaaat aggcatagaa taaccaatag acatttttct taagcaaagt
     2581 aacttatatt tgtagccatg gctaaaataa cccaaaatta aacttaagaa agaaggccac
     2641 gatagtctta cctttttctg aatttaacag aggatagatt gtgaataaaa atattgaacc
     2701 ctgaatgaag caatagaagc ccaacgccat tctcgctata gtgggaagga atttgtctct
     2761 tttcacttcg gttcatcagc atagtattgc taaaaaaaca ttttcagaac cattccccga
     2821 atttttttat ggcataaatt gggatgaaat cttatgaaaa gtttccattc aatctcgatt
     2881 tcgagtccag ttggtctaaa tggtccatcg gtccgtgggt ctttcgttcc attgattcac
     2941 aaaaaaatag ggtgttacga tgccggcctc ggtagaaggc tttgaattgt aaactatttt
     3001 taggggagtc cccagatgtc tggctcagac cttctccttg agtatctgac taaatacctt
     3061 cctatcctta tcttcgtcgg ccttgctatg gggtttggcc tggtcactct tgttctttcc
     3121 tatctggttc aacctaaata tcccgaaccg gaaaaacttt cgacttacga atgtggatct
     3181 gaaccatttt cagattcacg aatgccattt ccgattcggt attatgttat tgcaatgctg
     3241 tttgtgattt ttgatatcga ggtgatcttc ctttatcctt gggccattac gttcaatcag
     3301 ttgggcgtga tcgggtttat cgaaatgatg atattcattg gattatttgt cgttgcgtat
     3361 gtgtacgctt ggcgcaaagg ggccttggaa tgggattaat tcaaattgga aagccgccca
     3421 aggacgggga attgggggtc atgacccttt ccctggaaaa agtggtgaac tgggcgcgga
     3481 aaggttctct ctggcctatg acgttcggcc tcgcctgctg cgccattgag atgatcgcgg
     3541 cggtgtcatc ccgttatgat attgaccggt atggtgcagg agtgttccgc gcctctcccc
     3601 gtcaatctga tttgatgatt gtggccggaa ccgtctgtcg caggatggcg ccggttattc
     3661 gcaaaattta tgaccagatg cctgagccaa aatacgtgat atctatggga tcatgtgcca
     3721 catccggcaa tatctatgat agttatagcg tggttcaagg agtcgaccgg ttcgttcccg
     3781 ttgatattta tgtgcctggt tgtcctccaa ccccggaagc attgtttgat gggattttga
     3841 aattacagga tcgaatcatg cagaaacggg tgttcaccaa acaacccgaa caggtcaaag
     3901 tctaaggaaa cccacgaaac attcatgcat ccgatcgcag aaaaaatcca aagccgattt
     3961 cctgaagggt ttgtgggggc caatgaatgg cggggagacc tgacggttac cgtgaggcgg
     4021 gaagctttgc atgatgtctg ttcatatatt cgaaaagatc ctgacctgga ctacgattac
     4081 atggtacatg ttagttcagt ggattggcct gatgacgaag aacgatttga attggtctat
     4141 gaagtgtact caatccggaa acgccaccgt atccgaatca aaacccgggt tcccgaacac
     4201 gactgtctgg ttgattccat gacggatctt tggatgggag cggatttcat ggaacgggaa
     4261 gtgtttgaca tgatgggcat ccggtttaat catcatcctg accttcgccg cattttgatg
     4321 cctgatgatt acacagaagg atatccgctt cgaaaagatt tccccgttca ggggaaaggt
     4381 tggcgtgata cctttgattt tttaaacgac ccgacttagt tatctggtcc cttgccggga
     4441 aaaggccaaa aggtttaggt agatatcatg aagctggaag atcaacgtac gaccgtctac
     4501 aaagtcgatc cgaataaccc ggaaacggag tctttgccga cgcttcggac ggaagaactg
     4561 ctattaaaca tggggccaca acacccgagt acacacgggg tattaaaggt cattttagag
     4621 ctggagggtg agcggatagt aaaatccacc ccggtattag gctttcttca tcgaggagtc
     4681 gaaaaactgg ctgaagatgt gacatatcac cagtttatcc ctcatacgga tcggctggat
     4741 tatgtctgcg cgatgtataa caacttcgcc tactgtcggg cagtggaaaa actcatggat
     4801 attactgttc ccgatcgagc agagtatctc cggactctcg tggctgaagt gcagcgtatt
     4861 atcggccatc tgttctggtt gggcactcag gcgctggata tcggggcgat gaccgtgttc
     4921 ttttatacgt ttcgtgaccg ggagattctg ttggattggt ttgacgaatt atgtggtgcc
     4981 agactgacca ccagttggta tcgcattggt ggcgtcgaac gggacttcac cccttcgttg
     5041 atagacaaag tccaaaagtt cctggactac tttcctccca agattgaaga atatgtagtg
     5101 tttttggaaa aaaaccgcat ctggttagcc cggacacgcg gtgtggcggt aatctccgca
     5161 gaagatgccc tgagttttgg attgagcggt ccgaccttgc gaggatccgg ggttgattat
     5221 gatctgcgga aatatgaacc ctatagtgcc tatcctaaat gtgaatttag cgtgcctgtg
     5281 ggaaaaaatg gagacaccta tgatcggtac tggattcgga ttgaggaaat gcgtgaaagc
     5341 gtcaaaattg ttcaacaatg tttgaatcaa atggaaccgg gcccgatcat ggcggatgtg
     5401 ccgagtgtga ccttgcctcc taaggatagg gtgttcacaa atcttgagtc catgatccag
     5461 caattcaagc ttttttcgca gggatttgat gcgccggccg gggaaatcta ttgcggaacg
     5521 gaagcccata aaggcgaact gggattttat atcgtgagta cgggtggagg tcggccttac
     5581 cgtctgaaaa ttcgtgctcc ctcctttatt catatgggcg ccttcgatta tatggccaaa
     5641 ggctatatga ttgccgatgc ggtcacgatt tttggcacgt acgatattgt tatgggggag
     5701 tgtgatcggt agtgttgtct gctgtaccat gctttgaagc tatgttgttg gttttttcta
     5761 gaaaaggaca tcaatgggat taaagcctgt caccacgcct gacgtcgagg cggcgacgat
     5821 tgaactcacc ttggatggcg aagtcgtcca ggccaaggaa ggagtctccc tctatgacgt
     5881 catttccagc acgggtaaaa ttgtgcccgc gatgtgttac cactatactt tcgacccctt
     5941 cggatcctgt ggaatgtgcc ttgttcttca ggagggaaag aaagcacctg tccggtcatg
     6001 cactgcgaag gccgcggccg ggatggtcat ccagaccgaa ggagaagatc tgtttctcgc
     6061 caggaaaaaa gcggtggaga aacatctttc cgtccatccg ctggattgtc cggtgtgcga
     6121 tgccgatggt cattgcgaac tgcaggacat ggcctttcaa catggcgtaa ccaacttagc
     6181 caacgcgaag caaaaaaata ttccggaaga tacacgcagc ctggttcttg attttaacat
     6241 gaaccgctgc atcgcctgtg gagaatgcat caatatttgc aaggacgtac tacgaattga
     6301 tgctttgcaa tttatgaaaa agggcggttt tactcaggtg gtggcgaagg gtgatcaagc
     6361 cttggactgt gaattctgcg gtgattgcct ggcggtgtgt ccagttgggg ccatcaccaa
     6421 taaattctca aaatatgcct ataaaccctg gcagctcaaa aaaacgacca cgacgtgcaa
     6481 ttattgcggc gacggttgcc aaatttatgt ggaaaccaaa gatacggaag ttgttcgggt
     6541 gacttcccct ctttcctgga agaacaaatg gggagatcgg acagatacga taaatgggca
     6601 cgacggaatc tgtgtgcgtg gccgctttgg gtttcaattc atcgatagtg ccactcgcct
     6661 caaaaccccg ttggttcgta ccgaatccgg attgcagcca tccccctggc ttgatgcctt
     6721 ggatatggcc tcagggcgct tagcccatgt caaagcgcaa tatggggcgc aggcaattgc
     6781 cgggctcatt acagcccggt gtaccaacga agatctgtat gtatttcaaa aatttatgcg
     6841 atcggttatt ggcacgaatc atttggacag cagtgccaga tatgggcatc tgaattttgt
     6901 cagggccata aaacatgctt tgggccttca tcgtatgatg aatacctcgg aagagattaa
     6961 taaagcgaag gcagtcctca tcattggagc caacattaca gagaccaatc cggtcgcgag
     7021 tttgcgggtg aagtcggcta tggggctgta taaggctcaa actattgttg tggattcaat
     7081 gcaaaccaat atcgcgaaat tggcctctca ccctatgatg gtgaagccag ggaccgaggg
     7141 cctgtttatt caaggtctag tgaagtcggt catggatcag gatttgatcg acgaagaagc
     7201 cacgtcggca tttcccgaag ccttgaattc actaagacag gctgttggtg tgctctccct
     7261 tgaggatgtt gccaaccaga ccggtgtgaa tcctgaccaa atccatgagg ctgctcgaat
     7321 ttttgcagag gcgtcccggt cgatcatcat ttgcggggag ggcattctcc gacatgccgg
     7381 aggctatcaa catatgttga atcttatcga tctggcctgg gtaacaggaa agctcggtcg
     7441 aaagggatgc ggcataaaca cttataccga ggaagccaat gaacaaggag cggttgatat
     7501 gggcgtcgca ccggagtttc ttcccggtcc ggtttccttc actgattctc aagcccgaga
     7561 gacatttgct cgtatctggg gcaacaatct tccggatgtt gaccgtggag ccaatctcac
     7621 ggaaatcctt gaccgctgtc aaaatggtga aattaaggcg ctctatctgg ttggcgaaaa
     7681 tccattggag acccttcccg cctcctatga tgttacaagt gcattagcga agttggaagt
     7741 tctcatttgc caggacccct tcatgacgga aatcgccaaa atggcccatg tcgtttttcc
     7801 tgcttcaaca tttgcggaaa aagacggcac agtgaccaat caggagggaa aggtccaatt
     7861 tctccgtccg gctctagatt ctcttggcga aagtactttg gattggcaca ttatggtcgg
     7921 aatggccaac gggttgggct cgcccatgga atacgaaacg acccaggata ttcaaaatga
     7981 aatccgaaaa gtcctaccgg gttattacaa tctggggaag gttgcccacg aagctccaga
     8041 cctttcggga tatttctcga aggcttttcc agaggaggtt gcctcgcggt attccgtagt
     8101 gaggaagagt cccactcaac ggacttttgc cttacgcatg attcaattaa tttatcattc
     8161 gggaaaactt tccacccggg catccggcct catggaaatt tcaccaaatt cgtcacgtct
     8221 tcgaatgagc gcccaggact tcaatgatct tggtctgagt gctcatgatc gggtgcggat
     8281 cacttcagag cagggcgaag tggagatggg agtggaagtg gatatgtcta tgatgtcagg
     8341 gacttgtgcg gttcccgaac atttcaatga tccaccggtg aaagacctga tgtcgcatca
     8401 ggtcgatcct gtcacagggg ttccctattt caagttgacc tatgtttcta ttgaaaaggc
     8461 ctgatcagaa agaccagtca ccagacaatg agtaggtcta gcgagacaaa aaagtttttc
     8521 gacgccgtgc tgttcaaaga aatctggact gccatgaagg tgacgtttaa gcatatgctg
     8581 catcgcccta tcacctttca atatccacgg gagaaacgaa cgattcctga cgcccatcgt
     8641 ggcgcacttt gcctgctacg ctatgaagat gcgacagaac gctgtgtggg ttgcgatttg
     8701 tgtgaagcgg cttgtccttc acgctgtatt aaggttatca gcgaagaaga caaaactctc
     8761 cccctccaac ggtatgcctc cgaattttac atcgacatta cgaagtgcgt gttttgtgga
     8821 tactgtgtcg aagcctgtcc ggtaaatgcc ctggcgatga cgaaggtcta tgaattttcg
     8881 actcatgata agcggaccct cctctttgac aaggcgcggc tctaccaaat tggagagcgt
     8941 catatagatg atgccaaaaa gtatctctat gctcataacc aagagaagga aggggaggaa
     9001 ggccgcgcct accgctacca tttccctgtt tcaataaaaa gcacccagga accgtagttt
     9061 catcatgatg tggcttcttt ttggatattt tgccacggta agtgtcgtgg caggggtttt
     9121 gactgtggca ttgcgaaatg ccgtgcattg cgctatggcc ttgctgacgc tcctgctcca
     9181 tgtggcgggt ctgtttgtcc tgttgaatgc ggaattcctt tttgcggtgc aaatcatcgt
     9241 gtatgccgga gcgattctta ttttgtatct tttcgtgttg atgttgatga atttaaaaac
     9301 ggaggaccag catcttcata aaaaatatgc cctcctgttg tttgccggaa tggcaatttt
     9361 tggagaactt ctgattcttc tggtgcaatc tccctatgga ggtgtgattg gaacggcgac
     9421 gcctgacctc atcctgcaaa cggggcccag ccatgccatt ggaatcacga tgtttagtga
     9481 ttatttgttg ctttttgaag tgattgggat tttcctgtta ggggccgtga ttggcgcaat
     9541 cgtccttgct aaaacaccgg cgaagactga cccccaagaa gaacccgtca tttcttccat
     9601 gccctaaagc ccggtaacga tgattcccct ttctgcctat gtggcactca gcgcaatttt
     9661 gtttatgacc ggactcgtcg gcgtcctgat ccggaggaat ttcattgtcg tcctgatgtc
     9721 cgtggagatt atgttgaacg ctgccaatat caatttggtg gctttttctc attaccttca
     9781 ttccatgtca gggcaaatgg ccgcactctt tatcattgcc gtggccgcag cggaagccgc
     9841 ggtaggatta gcgattatca ttgtggtgtt tcgtggaaaa atggccacga acgtcgatca
     9901 aattaatatt ttgaagtggt aacgtgttcg atctgctcgt tctcctgatt cctctttttc
     9961 ctctgctggc cgtattggcc aatgggcttt tgggctctcg ctattcttcc gatctggcgg
    10021 ggcggctggc ttttgggtct gtaggcctgt cgtttctgtg tgtactcggc gtctttgttt
    10081 cgtttctcgg agattcaaca ccgcgcgaag tcatcgccta ctcctggatt ttcggaggcg
    10141 atctctccat taatcttgcc tttctcatcg atccgctgac cgtcattatg ctgttagtgg
    10201 tgaccggagt cggattcctc atccatgtct attctgtggg ctatatgcat ggggaagaag
    10261 gctttacccg ctttttcacc tatatgaacc tcttcatggt ttccatgctc ttgctcgtca
    10321 tgggaaataa ttatgtggtg ttgttcatcg ggtgggaggg cgtgggcctc tgctcctacc
    10381 tgttgatcgg gtattactat gaaaaagtct cagcggggaa ggctgcaacc aaagcctttg
    10441 tcgtgaaccg gattggggac gccgggtttt tactggcaat atttttgatc tttacccatt
    10501 tcggcacact cgattacacg gcggttatgg cccaggcagc cactctttca actgaaatgg
    10561 ccaccgcaat ctcattctgt ttgttggtcg gcgcgattgg caaatcggcg caattaccgt
    10621 tgtacacatg gttgccggat gcgatggaag gcccaactcc ggtgagtgcg cttatccatg
    10681 cagccaccat ggtcacagcg ggcgtctata tggtggtgag aaaccatgcg atatttgaca
    10741 tggcgccgat ggctttggcc gccgtgggca ttgttggagg cgtcacggca ttgtttgcgg
    10801 ccaccattgg attggttcaa aacgatatta aacgggtgtt ggcctattca accgtcagcc
    10861 agttgggcta tatgttcctg gcctgtgggg tgggtgccta tactgccgcg atttttcacc
    10921 tgatgaccca tgcgtttttt aaggcacttt tattcttgtc ggccggttcg gtgattcatg
    10981 cgttgggtgg ggagcaagac atccggaaaa tgggtggact gcatgacaag atcccgacga
    11041 cctatcgggt gtttctgatc ggaactctcg ccattgcggg aattcctcct cttgcggggt
    11101 tttggagtaa agatgaaatt atggcccatg cctttgtcca tggctattat cacttgtatc
    11161 ttctggcggc cgttggagcg ttcttgacca gtttttatat gtttaggctc acctacctca
    11221 cattctatgg gtcctctcgg gtggacccac atgtggccca tcatattcat gaatccccat
    11281 ccattatgac cgttccgctc atggttttgg ctggcttggc tcttttggga ggctttcttg
    11341 gggtaccgcc cgaacatggc tggttccatg gttttttgca tgatgtggcg actccgcttg
    11401 gtgctgaggc tcatgaggtg ggatggggca tgctgttggg cttgatgagt gtcgccattg
    11461 ctatcgcatt gggcggttgg ggtttagctc attacatgta tagcattcgt ccgcagatgg
    11521 ccaacgaatg ggccggaaaa tttccgcgag cctacaccac tattttgaat aaatattacg
    11581 tcgatgagat ctatgacttc ttgattgtgg aaccctgtaa aaagttgggg ttgctatggg
    11641 actggcttga tcgccaggtt ctggaccgat ttgttgtcgg tattggcaaa ggaaccgacc
    11701 ttagcgcagc agccattacc tggactgaaa agcacgtgat ctacgccggt ttgaatgtgg
    11761 tgggatattc caatcacctt ctctcgtggt cgtggcgaaa aatgcaaagc ggtatggtcc
    11821 atcattatgc ggccattata gtcattgggc tggcgctgct gattcatctt gtcctggtat
    11881 ggtttgtggg atcttctgcc gccgacatgt ccatccggta actgaacatc atgcaagaag
    11941 aattacaatt tggtttcccc attctgacct ttctaacctt tttcccactc gtgggagcgt
    12001 tggtgatctg ggcgctgaaa gaccaggatt tgatccgcaa agctacgctc ggtattgcca
    12061 gcctggaaat ggttgttgcg tcgttgatgt tatggcgatt cattcctgag acggcagcca
    12121 tgcagtttgc cgaacgacat gaatggattc ccttcttagg tattagctac cacatggctg
    12181 tcgacgggat cagcgtccta ttcgtaggcc tgaccgcctt ccttatcctg cttctggtcc
    12241 tttacgcctg ggatacggtt aagacgcgtc caaaagcgtt ttacatgtgc ctgctgggaa
    12301 tggaagccac ggtgatgggg attttcgtct ccattgattt gatcctcttt tttgtgtttt
    12361 gggaactcat gcttattccc agttatttct tgattaaact ctggggccct ggagaacagc
    12421 gccactatgc ggcgctgaag tacgtgctct ataccctttt agggagcgtg ttcatgctcg
    12481 tcgggttttg tctgatgagt ttgaactatt tcgaagcaaa acagttgtat agttttgatt
    12541 tacttgactt gctggcggtt cagattcctc ttagtcagca actgctgatt ttttggttgg
    12601 tatttatggg gctggccttt aaggctccga tctttccttt tcattcctgg ttacccgatg
    12661 ccctggtgca agggccgatc cccatgtcgg tcatgctggc cgggatcaaa cttggcacat
    12721 acggttttct ccgtttttcc ttccctctac tccccgatgc ctctcagcac agcacagtgg
    12781 tggctatcct catgactatg gggttggcgg ccattgtcta cggagccatt gtcgccatca
    12841 tccagcctga cttccgacgc ctgctggtgt ttagcagcat cagccatttg ggttttgtgg
    12901 tcgttggcat gtttgccctg aatttccaag gaatacaagg cagcctgata accatgatta
    12961 atttgggatt tagtacggcg ggattattct tcctggccgg ctttatctat gaacgtctgg
    13021 gtcataatga cgtacgacga tacagtggat tggcgaaaaa gatgccgctt ctcgccacct
    13081 ttatgctcat cattgggatg gcctcaattg ggttgccggg aaccaacggg tttatagggg
    13141 aattcttgat tttattggga gtctttcaag cctggtggct ctatggagcc attgcggtca
    13201 ccgggatcat ttttgctgca gcatactttt tgtggtttta tgagcggtct atctttggac
    13261 cactgaaaga cagcgtgccg gcagccatta aagatttaaa tagccgtgaa tgggcgattg
    13321 gggtggcatt gtcggttatg attttttgga tcggaattta tccttccccg ttcctgcgga
    13381 tggtcaatgg atcggtgcag gaagtggttg atcggttgca tcctggaatg gcgacggctc
    13441 atcatcgtga tgctctttta tccgctgagg ttaccgggaa ctaaatatta tgggtgaata
    13501 ttccttactc atcatactct ttgcgccttt tttcggcgcc attgcattga tgttcgttcc
    13561 tcggcaggaa gccatcctgg tgcgaatgac ggcggcgatt tctgcgggta tttcccttct
    13621 ggcctcattc tatatctttt tagcctacga cacgacgaag ggtggatttc aatttgttca
    13681 acggtatgtc tggtctgaag aattaggaat tgccttttac ctcggggtgg atggaatcgg
    13741 cggcccactg gtctttgcct ccgcaatctt gttgtttgcg ggcatctttg tttcgtggca
    13801 catcaaggac cggaccaaag agttctacat gttcctcctg atccttgcgg ccgcgaccat
    13861 tggggttttc atgtcgttgg acctcttttt cctctatttc ttctatgaaa tgtcggtgtt
    13921 gcccatgtat ttattactgg gcctgtgggg aagtcatacc aaggggtatc tcgccatgac
    13981 caatcccgaa gagcgaaagc ttcgggattc cataggattt attttcaact ttggatccaa
    14041 cagcaaagaa tacgcagcca tgaaactggt cctgttcctg tctgcggggg cggtggtggc
    14101 cttgatgggg atcctgctca tttaccatct ttccggtctc cacacctttg acattgtggt
    14161 gttgcgtgaa caggctcatt tttctgacaa cgtcgccacg ctaatttggt ttctgatctt
    14221 ttttggattt gcctctatcg ccccgatatg gcccttgcat tcctggtccc ccgtcgggca
    14281 tgccgctgca ccggcagcaa ccagcatgct gcatgctggt gtgttaatga aactcgggca
    14341 tttttcgatt attcgtgtgg cctttgaaat cttgcccgat gcgacacgcg agtggatgtg
    14401 ggtggcggct gtcctctgtg ttttttccat cgtctatggg ggactggtgt cttatttcgc
    14461 caaagacacc aaatacgtca ttggttattc cagctccagc catatgggtt atatattcct
    14521 gggcatggcc gcattgagtt acatcagtct atccggagcc gttatttata tgtttgccca
    14581 tgccctggcc accagcatgt tgtttgccat ggctggctgg gtgtacgacc aaacccattc
    14641 acgagacatt ccctctttgg gcggtctcgt tgagaagatg ccgtttattg ccggagcctt
    14701 tattgtcggc tgtatggcct ccatcggcat gccgggcacg ataaatttca ttgctgaaat
    14761 tatgattatt gtggggagtt gggataaata tcctttccag gttctcgtgg ctgtgctcgg
    14821 catcgttctg acccttgcgt atatgttcaa aatgatgcga gggctgtttt acggaaaatt
    14881 agatcctgaa tatgcccatg ccagtgatgc cagacattgg gtggatcgca tgcccttgct
    14941 tcttttaata gtctgtagca ttacccttgg gattttcccc gggcattttt atgaagtggt
    15001 acggtcaacg gttgaaccca tggtagaccg cattaatcat gtggccccac tcataactca
    15061 aagcaccaaa ggctttttgc cctaagcgct ataccattga accttctgat gactttctaa
    15121 atacttgcaa tgacctttca acttgacctt tccgtcgctg atctgctcct gcttctgcct
    15181 gagatctttc tcacgttgtg gttgagcctc cttcttgcca tggatttttc gctgaaacgc
    15241 ttgacccacc aacaattggc ctatctgagt attgcgggga tggcggtcac cggtttgatc
    15301 cttttgggat ttgaccaagc cggaaccaca ggcgccttgt ttaaaaacat gttcgtgctg
    15361 gatcagatgg ccattgtctt taagctcatt attgtgggcg ccacggtcct tgttctattt
    15421 atgtcgatgg attctgtgca ggacttcaag tttttccgag gcgagtatta tgtcatggtc
    15481 gtcatgtcgg ccctcggcat gatgttcatg gtttcgtcca atgatcttct ctccgtcttt
    15541 gtcaccctgg aattttcaac ttttggattt tatgtgctgg tcgcctatct cagagatgac
    15601 caacgctcat cggaagccgg actcaagttt ttcattttag ggatatttgc ggcgggatta
    15661 ttggcctatg gcatcagttt ggtctatggc gaaaccggga ccttgatttt ctctgaaatg
    15721 accagcactc ctggaagtta tgggttggcg attggctaca ttttgatttt tgccgctctg
    15781 ggattcaaaa tcggcgcagt cccttttcat gcctggattc cggacaccta tcaaggtgcg
    15841 ccgactcctg tcacggcctt tctctccatt gcgccgaagg ctgcagcatt ggctatcctt
    15901 gtccggatgt tttatgtgtc tttggcttca ttcaagccga tgtgggcgtt gttatttgtt
    15961 gtcgcatcca ttttctcgat gacctacggc aatatcgtgg ccattgccca aaaaaatatt
    16021 aagcgtctgt tggcctattc cggcattgcc caaattggaa atatcatgat cgggttagcc
    16081 gcagggacga aacaaggcag cgatgcgatc atgttttttc tcctgacata tctttttgcg
    16141 aatttgggag catttgcggt catcattgcc gtcagtcagg tcatgaaaag cgacgaaatt
    16201 gaagattaca atggtctgaa tcgacgctcc ccattcttgg ctgcggccat gttgcttttc
    16261 ctgttatcgc tggctggagt tcctccgctt gccgggttta tcgggaaaat ctatttattt
    16321 attgcggcaa tgaaggaaga gctgtatacg ctcctggttg tgggactgat caatatcgtc
    16381 atctccatgt actactatct gattgtcgtt aaaaaaatgt acatcaacga acccactgat
    16441 ccttcgccaa ttcatgcgtc catgccaatc aaagttgcgg tgtacgtcgg tattgccgga
    16501 actgtgctgt tgggtgtgta ccccggcccg tttattgact gggtcgtcgg tgcctccctc
    16561 atgttctcca atattgcagg gcccaccgcg gccgcctctc ttcttcccgg cggataatcg
    16621 ctagcgtctt gttttcgcct tcatcctcct tcccggattg acgagcgcaa ttacttttcc
    16681 aatatttccc caggacctcc ttgggaggaa ataatgtgag tcgggggtga ggatattgat
    16741 gaagtattgc gaaaatggaa atccggtgga gttacattcg ccaggaaagg atggtttcca
    16801 ggggattgag tccattgagt tgccaaatgg ccccgaacac aacggataag gtcacaatca
    16861 ccaggaggat tagacctatg aggatcgaat ttcttttgct acgcggaaaa cgggggggag
    16921 cgactttctg tgaacccgat gggtcttgcc acaatgcaga gggtcttcgg ggtcctagaa
    16981 gtgctgtggt ttttcggaga ggttccactt gacgttctcg tgggcttttt ggggctaccg
    17041 gtttccttgg tgtgaatgag ggtgtcttag cttgtgactt gtcatctgtc tttaccgatg
    17101 tgggaacttt tctggtatgg atataggatg ctcgacagtg cggacagacc agtgtttcct
    17161 ggattgattc cccattgtcc tcctgctgca cctgtcctcc cgttagtgcc acccggcaat
    17221 atgggcaagc gggggcgtcc ggcgagacca aaccgtgaca ttgtggacag gtatgtagtc
    17281 cggctgcacg ctcctcctcc aatgcgtgtt tcccaggaga agggaaagcg cattgaggac
    17341 atgcccgagc ttcggtcgag acctctttgt gacattcagg gcagggaaca agggccatgg
    17401 agtgaatttc cgttctcgct atatcacacg tttttcgaac atacttcgta aagacacagt
    17461 caatccctag aaactttgcc taattttatg agtaaatcca ggtgagtcct cctcctcctt
    17521 gaaccaattc agaggtaaat tgatgggtcc agcctgcagc tattctctct tatctgattt
    17581 ggtcttctca agggagaggg atttttttta aatccagtct cctcatctta ttctgtgttc
    17641 tctttcctgg ctaaaatgtg taacatttct cggcatgtca ggacaggata atcagagtgt
    17701 gaactcttgt cccgggatga caggatgcaa ggaacgaaca ggattactca aattttcatg
    17761 agggtactat ggccaatgaa acagaagagc aaaatcattc tatcacgcag actgtcagtc
    17821 aacgctatgc caaagcggtg accaacggtg agcagctgtg ttgcccgacc ggctataatc
    17881 atgaggattt gggccaattt attccggaac cggtcttgaa ggtgtcctat ggctgtggaa
    17941 ctccggttgg cctttccact gttcaacagg gagaagtcgt tttggacatt ggttcggggg
    18001 gcgggatcga ctgctttgaa gcgtcccgga aagtcggggc ccaaggccgt gtcattggaa
    18061 ttgatatgac cgatgaaatg ttggcgctgg cccgtacgca tgccccaatg gttgccaaaa
    18121 atctgggtta tccggagccg aatgtggatt ttcgaaaggg ctttgccgac gccatgccgg
    18181 tggatgacga tcaggtggat ctcattgtct cgaattgtgt cattaatctc gctccggata
    18241 aggggaaggt ctttcaggaa atgtatcgcg tgcttcgacc tggtgggcgg ttcaccattt
    18301 ccgatattgt tgccgaccaa cccattccga attacttaat ctatgacaag gccaaatggg
    18361 gcgattgttt gtccggcgcc ctcaccatca aagactattg gggcgggctt tgggacgccg
    18421 gcttcaaagg agttcatctg gttaatcaca ttccatggag agtgattgac gggatccagt
    18481 tcctctctgt gacgcttacc ggatataaac tggcttcggc atcgacaacg ccctcttcaa
    18541 cctttgcgac cctcaccgga ccgttctctc aagtggtcga tgacctgggt tcaacattta
    18601 cccgaggcca tcctcagcaa atcaacgaac ggacggctgc attattggcg ttagctccct
    18661 atcaagacct gttcattatt gacgagaaac cggtggctct ggcggttcaa gatccccgca
    18721 tgcgggccgt ctatcccgac gaagcgcctt gtgtctggga gggacagttt gccgtattga
    18781 ccggaccgtt cctagcggtc tgtgacgacg accaccatac gtatcaatgt ggtgagccga
    18841 tagaaatttg ttcgaaaaca tacaaggtcc tccagaccga ttcttatcaa ccctattttg
    18901 caactatcaa tcgatccagg gatggggtgc attccgaacc tgtcatttgc ggaacttcgg
    18961 aggggtgttg ttaagaacgg tgtgaagtcg ccatttgtgt gtaaggaatt aagaccatca
    19021 tgacgatgaa aacttcagct ctccccatgg tcgccccggt tcaaccttca tggatagctc
    19081 cgtcgttcga gcaagcgttg gccctccatc attcgcttcc cctcaaggcc caagtaattt
    19141 ccacgcttca ggtcaatgtc gggaaggtct gtaaccaaaa ctgccaccac tgccatgttg
    19201 atgccggccc tcaacggacg gaaagcatga gccgaaccac catagatcaa atcttggacg
    19261 tgctcgatcg aactcctcag atcaggacgg tcgatatcac cggtggggcg cctgagatga
    19321 atccacactt tgagtatctg gttgagcaat gccgagctcg agaccgcagg gtgatcgacc
    19381 gctgtaattt gaccgtgttt tatgtcaagg ggaaaacaca tcttccccga tttcttgcag
    19441 accatcgtgt tgacatcgtc gcctccctcc cttgttatga agcagacaat gttgatcagc
    19501 aacgtggaaa aggcgtgttt gatcggagta taaccgcact gcaaactctc aatggcttag
    19561 ggtatggagc agacggttcg ggtctcatgc ttgatttggt ttataatccc ttgggacctg
    19621 tccttccccc gcctcaagtc gaacttgagc aagatttcaa agatgaattg gggcagcaat
    19681 ttgggattca gtttaaccgg ctctatacca ttaccaatat gccgatcagt cgtttctggg
    19741 atgaattaca cgatgccgga caactggaac attactacac gctcttgttg aataatttta
    19801 atccattggc ggtcgagggg ctgatgtgtc ggtctctgct gagtgtcggg tgggacgggc
    19861 gcctctatga ctgtgatttc aatcaaatgt tggatttgcc gatccagccg gattcccagg
    19921 cctggatagg ccggtttaat ctgtcggaat gggaacatcg ttcaattgtg gtcgggcccc
    19981 attgtcttgg gtgcaccgcc ggatccggat catcctgtgg gggagccttg acctagattc
    20041 cggcctggca cccccacatg gatttttcga ataccttttt tacgtgacat cttccggctt
    20101 gaaatgcctg gcgcaatcct ggctacagaa ataataattt ttcccttttc gctcctgcac
    20161 aatggccgta ccggcggcca cgtacacttt acagacggga tcttgaatca tcacgtcttt
    20221 gcctggggtg gcggggtcta gtttgggctg gctccattca cgtgccgccc ggcgaatcat
    20281 aaagaagaga acagctaata aaatcagaat gattataatt ttatacatag tgtgatcagc
    20341 cttttgagat ggatagcatt ttgtaccatg tttccaggtt tgattccaca tagtccgtta
    20401 gcggattttt ggactaagtg tgttgattcg catggcattc ttccattgct gtgccctttc
    20461 tcaacttgtt aggattttct tggtgttgct gtccacttta tttggggcca ataggtttcg
    20521 cagtccatgc tgattcgagc catactcata attctcgcag cgagtggttg caccatgaac
    20581 cctcccttta cgggcacaat ccttgatctg acctatccct ttaatgagca gaccgtgtac
    20641 tggcctacga atgagccatt tcattggaag aaaacaagtt gggggcctac ccaacaaggc
    20701 tattggtatg catctgcggt gtactcagcc tctgaacatg gagggacgca tatggatgcg
    20761 ccgatccatt tttcggaatc aggctggagc ctggatgaaa ttcccgtcgg ccagttaaca
    20821 ggtgaagctg tgctgctgga tattcgagcg caagtggatt caaacccgga ttatactcta
    20881 caggttgacg atatcgccca atgggaaatt tcctacggac caatccctcc aaaagccatt
    20941 gtgttactct ttacgggatt tggacaatat tggccggaca aagtccgata tcttggcagc
    21001 tccaccccgg aagatcccac cacactccat ttcccagggt tctctgcagg cgcggtaacc
    21061 tttctgctga cgaaacggga aatattagga attggcatag atacagccag catagatgcc
    21121 gggcagtcac gagactttcc ggttcatcaa attcttggtc gagcgaatct ttatgcgttg
    21181 gagaatgtgg cgcaactcgg acggttacca cctcgaggcg ctcgcgtgac ggccctcccg
    21241 atgaagattc aaggcggttc aggaggaccg gttcgaatta tcgcaaccgt gccgtagggg
    21301 ctgtttattt caggtcctga acgaaagatg ttctcgatct ctcaaataca caggcattat
    21361 tgatgatgaa caaaaggaaa ttatggttac aattacatta atgggtgatt tgcaaaccgt
    21421 tgatggagag cgagcccttg gatgcgagat tgctgactcc atctccgtaa agcaactgat
    21481 taggaaacaa gggaaacccc ttcgggaaat ctttcagttg ttaaaagaaa aaaaagtcat
    21541 ggtgaccgtg aataaacgaa ttgccagtga agaaactaaa gtctacgatg gagatgccgt
    21601 ctatattgtc gctcacgatg gaatgggaca aagcggactc acgctctcgc attactaatt
    21661 ctagatgacc atgttgatgg gcttgggcaa accgaagtct ccagtggttc ccccatgggc
    21721 ttcagtcttc tggcagggac ttctgacctt aggcggacct cctgtcagcg tgtcaaaggg
    21781 ctcaaaatcg tccatccccg gtgagcgatc acgtacgcaa aaaaagggag ccgccttatt
    21841 cgaacggctc ccctcaaatg ttggaatctg attactcttc agtgatttga atcttgcgag
    21901 gtttggcagg ccccaccttt ggcagagtta ctcgcaatac tccattattc agtttggctt
    21961 caatatgttc ctgatcaatc acttcagaaa gagtaaattg gcggaaatat ttcccggtct
    22021 cgtactcgcg taaaacatgt tcctctttct caggaacaga aactgacggc acaccgctta
    22081 acgaaagcac atcgttacga agatcaattg agacattttc ggatgggacc ccgggcatat
    22141 ccgccacaat tgtcagggcc tgatcgtctt caaatatgtc aacattgggt gaaaataccc
    22201 gaccagggat tgtttgttcc gcatccgatt tgacttcccg tttatcccgc acttgtatat
    22261 cttgcgtctg tgttgtcatg tgttcatcct cctttgaatt ggatggggtc ttccccatcc
    22321 ctcacttcta tttcaatctt tatcctgtaa tggcgatttt tttcggttta gccttttcag
    22381 atttaggcaa gaccaccatt aaaataccgt gttgatattt tgcttgcact cgctcactgt
    22441 ccacatctgt gggtaaagtt aactgtcgtc ggattttccc ggcttcccgt tctcgacgat
    22501 gatatcgcac attagattgc tcatccggaa ttgcccgctc acctgtgacg gtaagatttc
    22561 gacctgtcac cgacacctcc agttgatcga gcgtcatgcc tggaacttcc gagcgaacat
    22621 aaacattttc gctatcctgt gacacattca taagcggata gacacctgct ggagaatccc
    22681 aaccattcac cctggatagt ccattgaaca agtgcccgag atctctccgt agccggtcca
    22741 gctctgagac tccgcccgaa aatccattga tcggccaatt ttccaatctt cgaatgacca
    22801 tagcttaccc ctcctctttg aacttctcgt actccattct tccaaatgtt gttaattcgt
    22861 cgggatacct tcgaaggtca aacatgcatc gatgttactt ataaaataat ttcaaaattc
    22921 cgggagtcaa gtaggacgat aattttctgg ggaaatgcgt aaaaggagtg ggagattgtt
    22981 cggtacacag attgagctag gaacaagaat gaaagcggaa aaatgagaaa cacagggcgc
    23041 ccaatcggcg taattgacct gaagaaagcc atgaccgcaa ccctctcgct tcaaccgtcc
    23101 tgccttttag tgaatccgaa gagaaataac aaaactttgt aatggcgtga ctgtttgtct
    23161 gaaaaagaaa agacttgtcc tgatcgtagg gggaattatt cgccgacata gggagaatgt
    23221 ttttggaaaa cgtctttgcg taaaccgaat ttgtgttggt gcatgggcgt tcgggccatg
    23281 acgaccatgc gggtgaacaa tatggccgct attgacattg ttcaatccaa atcgtgaatg
    23341 tattcctttc tcaggagtgt ttcgcggtct gcgggagggc gctcaccgtt tcataaggag
    23401 gatactatga atcagcgatc tttcggtctc ggattactgt tcctcatgat gttggtttgg
    23461 agtggatgcg attccggcga atacggaaaa gaaacgaaac cggaacccaa agcggaaagt
    23521 acaaccatgt cgccggccga agctccgagc atggcggaaa ctccacaggt tgaaggcgct
    23581 ctgatggcaa tggccggtgt cgccggtgca gcagaaaatg aggaaggggt caaccatttt
    23641 cagcaggggc attgggacgt ggcccaagaa catttttcga aggccatagc cgcgaatgct
    23701 gaccttccgg aagctcatta caatcttgcg ctcgcattgg ataagttggg gaaccacggt
    23761 gatgccacga accattttaa aaaggccttg caactggctc cggctgatcc gttgattgcc
    23821 ggttctcaaa tccttcaggc tcatgtgggc gggtgaaatc ctcacggtta gagaggtcca
    23881 gcaagacatg ggagttgtca tagttttttg gacatccttg tggctcccga attgaagccc
    23941 gacgctgtgc tgaggcccgg tagaatgttt tccaccggtg ggaagaatac catgtcttaa
    24001 ctgttcctag cttttcttaa aaccggaatt gcgccgtgaa tggttgtcct acttccacgt
    24061 cacctcctgg gacaggtttc aagccacctc aaaatccctg agggaaattt gcgaggctct
    24121 ctccccatgg atgcgctgta ttatcccttt cacctttgtc atgaaaagac tctccatcag
    24181 cttttggaga actattccca tgtccatttt ctggatttta tggccttgca attaacccca
    24241 ttcatggggg caacagggtt tccggaccgg atgggagatt attatccgga tcttttagag
    24301 agcggtagaa tcatccaagg tcataatgtc agtgggccgc tgagtggggc gaaggttgcg
    24361 gcggtcaatc aggatctcgc cgatcctctt tggcgatcga tttttcatga ggctttgaag
    24421 gatgatcgac ggtttcagcg agggttggtg gcattttccc aggatagtca aggtcgaagt
    24481 tccgtcccga ctgactctgc actcttggtc gcatttcaac agccagaaag ggagttcgtg
    24541 ccttacaccg ttgaaaccct tcaagccatg agccgaagac ggttgcctga acgggagagc
    24601 cttgcatttg agtatgggtt tggattgatt aaaacgtctg ctgcagtgat ctacagtatt
    24661 cggcgatgcc aacaattggg ctcggtcgcc gtcaccgatt cacctgggca tcatcagctc
    24721 ctgacaagag catgtcagcg ggatagcgtg gagttaagca actactgtgt aaagcgggaa
    24781 gggtactgag ggggactgtt gaaaaagccc accagcggcg ttttcagtgc tcgaagggct
    24841 caacgtacct cccggtacgc ctcgccctcc tcgctgcttg cggccttgct ggacggccat
    24901 tttgaacagc ctatgaatct atgaagagcc aagccatgga tatcgaaaac cctaaggtgc
    24961 aaaacacttt ggctaccggg gttcctcgtc gcggatccgt agaaaaaccg gaaagcgagg
    25021 gatgccgttt tcggtcaatc cctggtgacg aaaggtgacc cggctctgta acggtggcgg
    25081 gcgacgccgc tgctcatgac tgagcccgct tccgataaag aagatgaccc catcatcggt
    25141 gcgtaccttg agtgaaccaa tctgaccggc aaactgcccc ttgcctggcc gatatccaat
    25201 gactgtcgct tccgcatcag tgaataattt caacttcagc aaatcctgac tacggccact
    25261 ggcgtacaac gcagtttccc ggtgaagcat caacccctca ccaccttcat cggtcaccgt
    25321 gtgcaaccat ttcaacaagt cttcttcgct cccaatccgc tgttgttcaa tgattcccaa
    25381 atagggagcg ctgccttcga tcttgaggcg attcatctcg agcacacgtt gggcaaatgt
    25441 tccgccatgt gccggaagat caaagaccat caagcgcaca tttcgccatc catcatgggg
    25501 ttgttgtgtc cggacaatgg acgatacttc ttcatatcgg cttcgcccta tccataactc
    25561 tccatccaag ggcacggctg gaaacccttt ggtaaaccat gccggtgccg caaacacatt
    25621 gccgcctcgc gaaatcaaat gtgttccatc ccaacgagcg cggacgccgt cgagtttttc
    25681 gctgacccgg tattggctga gagtaagacc ctgccgatag atgtcagcca acatcagttc
    25741 gggtatcgtg ccggcttgtg tgacagagag gggaaggaaa atgatcagga ggatggccgg
    25801 caacaggcag ttctgtagct tgcggactgc ctccgatatg cccaagcgat catgcgcatg
    25861 ttcggtgggg atgatccaga aacagggttg cctcattcct cctctcgggg aagggtgatt
    25921 tgtttattcg ccgatcgtgt caatcagttc gatctcttca ggcaataatg agaacgattc
    25981 ggcctgaaga tcctcttcca tatgttgttg gctggtcgtt cccgtcaatg ggagcatccc
    26041 aatttgcagg gcaaagcaaa aaatcacttg cgcgggattc gtccccaatc gtttagcgat
    26101 ggtccgaata tcggggtcgg ccatcacccc tctgttggcc gtcaggagcg aaaagccctg
    26161 gtaaatgatg ccatgggttt ggcaaatccc cctcacttct ctatcccaac catgggaggc
    26221 ataacaccgg ttctgcacga ccatgggttt gatccttgcc tcttcacaca gttgggtgag
    26281 ttgtccggcc gtgacattgc tgataccaat cattttggtc ttcccggatt gatagaggtc
    26341 ctccatggtc gcccacacct cacggtcaga atctcccaac ccaaatcgtg agtacggacc
    26401 atgcaaaata tacgagttca catagtcggt atggaggtgg gtgagcgagt tggcaaatga
    26461 ttgctgcacc tgagtggtga gatcggccga ggcatcatag ggagtgcgat ggtcctgtcc
    26521 gttcaccggg gtaaatttcg tttgcaaaaa cagggtttcc cgcttaactc ccagcttgtc
    26581 gagtgccagc agagcttcac ccaccaggtc ttcccggtaa tgaatgagct ggttggccgt
    26641 gtcgatggct gtgaaccccg accccaccgc caattgcacc agatgggttg tggcgccttc
    26701 tttccacgcc gtgccataca tcaatgaggg aagtgggaca ttattataag ctgttaaagt
    26761 cataacgatt cctccagatt caacgactta cccgaccttg tcttcttcct attctagcga
    26821 cttcgggtgt atttttcagg tattcaggca gtatttgggg agaaatgaaa ggggccgtgg
    26881 aatttgcctc cgttggtatg ggggtattgg cctgcctggg caccacatat tccgggagac
    26941 gcac
//
LOCUS       DAORAQ010000022        26786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_349_length_26786_cov_65.328383, whole genome shotgun sequence.
ACCESSION   DAORAQ010000022 DAORAQ010000000
VERSION     DAORAQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 26786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26786
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_26786_cov_65.328383"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKM72_03735"
     CDS             <1..326
                     /locus_tag="PKM72_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNP59925.1"
                     /translation="NLELRAGLSREQGLRNLVNRTNLDDIRSLVALLIQTDRFGTSIG
                     QALRVHSDSMRLKRRLKAEERGAQLPVKLMIPLILFIFPALMVVIIGPGAISLLRNLM
                     PAMGG"
     gene            503..1498
                     /locus_tag="PKM72_03740"
     CDS             503..1498
                     /locus_tag="PKM72_03740"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP59926.1"
                     /translation="MMKASLLVTLVVLFSLGVVGCAKKEQSMFTLQDEHYQQIMERQK
                     AGIDSESQAPVDLTQVMKGEEYEKLGDAHLQQGDFRTAKIQYEKVLEASPEQIPARYK
                     LAVIYLEKGSPQEAYNQFHQILDYDLNFAPAYEGMGRALLKMEKDQEAEREFQAALLY
                     DGEMWTSQNYLGIVADRRHDHKEAIHLYEITLQKRADDPSILNNLGMAYYLDNQYEEA
                     VRTFQHAIQVGGSNDKISNNLGLALTKLGHFDLAYDAYARGMDPAKAYNNLGVAFLEI
                     GKFARASRCFEKAIETQPTFYEKAKENLLLSNRMLSKFPMVQQQTLLRRDPVCVS"
     gene            complement(2147..4855)
                     /locus_tag="PKM72_03745"
     CDS             complement(2147..4855)
                     /locus_tag="PKM72_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP59927.1"
                     /translation="MDRQHFFQELNQMDISGLEKALRSSQAFVHTLMDSIHIGICHVN
                     TQGQILSLNLEGARILHRTETSCLGQSFHAYAECLYIDLGTQEEHCPITRAITTGKSI
                     WTPQLLIRRPSGETRWVEFQSTPLTDPRHSGALIIFRDLSLELQQHERHHHLASMPEE
                     SPNPIVEVDAQGHLAYANPAMIRLLDTYGFREDGVPMVLPSTLTQLTLECLKTITPIR
                     GLTVIVEGHHFDWTFSPLQEKGLVRGYGLDITHHVRVSQTMTELHSQFSSLVDSAHEG
                     IISADLHGTIVSWNPAAQSMFGYQPDEVLGHSIVTLLEEGYRDMYRKGLGDISYGRPS
                     TFPLQQPLELTGLRKSGESFPLEISSTSWKVGMDTHHGFILRDISDRKRLEQALIAEK
                     DHLVTTLQSVAEGIITTDREGRISFLNPLAEQITEWTTHEALHRPFQEIFRLTGDSKK
                     NDAEPSLQFSSRTLVLSELDAPQQLLTKGGRQRKITVREGPIRDHSGHLVGTVIVFRD
                     ITDQSRQLEEQQRISKLNSLGVLAGGLAHDFNNILTTILGNVFVAKLRMVSSDPLTQN
                     LEQAEQACLRAKELTQQLLTFAKGGAPIKTSIALGELLRKSTIFALSGSSISCHFDIP
                     DDLWPLDADPGQIRQVFQNITLNARQAMPHAGHLSLKVENVRLNDSSALPSPALIPGN
                     YVRISFEDQGNGIEARQLANIFDPYYTTKPGASGLGLATAHSIIQQHHGHISVTSKVG
                     VGTIFTLFLPSAHVTPEVETENIPAIPRNYRGRVLVMDDEHSIRRMVEDALTQFGYDV
                     VSAPDGQTAINLVSKGLADGKKFEVVILDLTIPGAMGGVKAIQHLRKLDPRIKAIVTS
                     GYSDDPIMCDFQEHGFQGILVKPYKIFDLAKTLECMFSQNKGETTH"
     gene            complement(4908..6131)
                     /locus_tag="PKM72_03750"
     CDS             complement(4908..6131)
                     /locus_tag="PKM72_03750"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNP59928.1"
                     /translation="MIKRISIQDLRPGMYITGMDRSWFKTPFLRHKWTIKREDEIALL
                     RNYGIKEVQIDTEKGCDIAKTSGGQHTSEDQDLSPTTPLTSPGVGSGPNPEDIEAARL
                     LRAEAISTMEVFFHQMEAPTPQHVQEVRSVVSILLDGILDHQAAMVSLIQMRRFDPNL
                     ASHGVDTGMLAMAIGHEHGCDPTQLKLVGLAALLHDVGQLRLPLNMIRKVERFTPQEQ
                     KLMHAHCEIGVAILKQFPDIPDESKQMVLEHHERLDGSGYPKGLRGLQISELTQILSI
                     ADTYDAQISGRCSCPPVPPARALSELYQAAVEGQYHLYLVQQLIQLLGVYPIGSLVRL
                     NTGEQAVVVWVSSHSRMTPTIKLLKDSLDQPYPEQAIIDLAAQSPTTPFRSIEKALDP
                     SREGWDMSKILEALW"
     gene            complement(6358..7050)
                     /locus_tag="PKM72_03755"
     CDS             complement(6358..7050)
                     /locus_tag="PKM72_03755"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP59929.1"
                     /translation="MTENVQKTTGFSHHGSQLLTRVVLHLLGSPVRREDSSPTFMKHP
                     AHRTLFLWWLLAGLISLLTGCPTSSEWDRQWEQVHANITRGQFQEAKDLLHGLLPSVR
                     EHGPTDERYALVIFQLGEVARLEGEESLAEAYYWEALPLLAQSLGAEHLRMADPLAAL
                     ASLYQQKNQLEMARPLLKRALALREKSWGLSDPQLLSTLQNYHALLLAGDHQKEATEI
                     AGRINRILKRSS"
     gene            7095..8567
                     /locus_tag="PKM72_03760"
     CDS             7095..8567
                     /locus_tag="PKM72_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNP59930.1"
                     /translation="MINQVLRYCQDDVWKEDVNALTGFRQFGVKFLRLALVAVAEFQQ
                     SALSIRATSLVYTTLLSLVPFLAVTFSVLKAFGIHQKIQPILAQALDPLGPKGVEITT
                     NIIQFVDNMKVGVLGAVGIAGLFYTTYSLIEKIEEALNSIWRVETGRPLARRVTDYLS
                     VVLVGPVLVFTAFGLTASAQSHWLVQKILAVQPLGHLMVVLTNLLPFLFMCLVFTFIY
                     KFLPYTNVKLNSALVGGVTASLLWQVAGLAFASFVVGTGRYSAIYSGFAVLILFLIWL
                     YVGWLIVLAGAQVAYYHQHPSAYLLHLSRKKQTPLFREYLTLSLLTHITRRFLLGKPP
                     LRAEQLARAQGVPLTLVESLIDDLVKGQMVVKGDDPKGLVLAQAPENIQVVEVLRVVQ
                     HQPHSGSQAFTVGSDNISRVLMHRDAAVEAALEGMTLRDLLRRQSEIVEEEVRTDHPE
                     TQGSGGAFHPESECGHSVSPDYLQETRQTDPSVVGKGPAR"
     gene            8595..10043
                     /locus_tag="PKM72_03765"
     CDS             8595..10043
                     /locus_tag="PKM72_03765"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNP59931.1"
                     /translation="MNMWIVPGAPLKEGQGWRIWCSQKGEGSFSPPQVEVRQKNATVP
                     SRHTWTLLPQLPGLKRRMGVMVLTLSTSGPPEGALYSVILSANQQNRVFQWWTMPYHL
                     AAQPVTFLFASCFWHNNDREGYYRSGLQELGQLAHPHFKLLLGDQVYGDWPNAWDLGD
                     EGIELYAKRYAQYWGDEAYREALQVCPNFFTCDDHEYWNDYPEKQIQLPQTWASKNRK
                     TYGGGAEDLYYQYQRCLNPDEARWYRFEIDPVSFFITDTRSEREKCNDKGTSHFMSKE
                     QWVALEAWQQGLTGPGVLALGQPLFQKDGDFRDHSLSNFKQDYARLWRVIERSLAGQN
                     SQHQPHDILILSGDIHTGRYAEAHGPFPDAPYGVPEFIASPAAMINPGNSRPEAPPEK
                     IRILPNAEGKESVWRIDPHSNIDIMTIQNNVGLVRMVPGSQTGKSPRVRFELELWSLP
                     SKVIEIDWDETAPPQGLGGPLKRLWKKELLLR"
     gene            10127..>10908
                     /locus_tag="PKM72_03770"
     CDS             10127..>10908
                     /locus_tag="PKM72_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59932.1"
                     /translation="MRHSQTSTRNPDIKDWTSKYGKRTNLQFFNDAFAYLQKQDIGNR
                     GLMTVGLIGSRDVPGHTLRTAQSTVRWDLRPSFWSHVFVVAEPVTSRTSLRSLPILEI
                     PLHPRNGIFPRPECNGINEGTLGLYEDKDVDANVGLVAVSMSDEDAKKLKKRAMNWNQ
                     DRVRYNFWEMLGVWQSYLWSQGAKRNPLREGTPIAASSYIEFIFEGLSLGVTPGTSER
                     NSAPEHLWNAACWWHKAFKEQDRKVAGVFVARDRGCAMAGCD"
     assembly_gap    10909..11008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11183..11338
                     /locus_tag="PKM72_03775"
     CDS             11183..11338
                     /locus_tag="PKM72_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59933.1"
                     /translation="MTPCFLVSRSAVATFTGESPIVCCAVSPDGRTIVIGESSGRVHV
                     LRLEGID"
     gene            11568..12443
                     /locus_tag="PKM72_03780"
     CDS             11568..12443
                     /locus_tag="PKM72_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNP59934.1"
                     /translation="MMKIGLIQMACSANSAENLERAVAKAEEAAKGGAQIICLPELFR
                     SQYFCQKEDADLFDLAESVPGPSTEAFAKIAKSHRVTILVSVFERRTAGVYHNSIAVL
                     DERGEIAGLYRKMHIPDDPAYYEKFYFTPGDRGFQAINTTHARVGTLICWDQWYPEAA
                     RMTALQGAEILFYPTAIGWHPKEKSEEGQAQREAWMTIQRSHAIANGVFVVAVNRVGH
                     EAPAGGDGLEFWGSSFVCDPFGVVLAQASFEKEETLVVDIDLKRIEEVRRNWPFLRDR
                     RIDAYGGITKRLLDE"
     gene            12901..14457
                     /locus_tag="PKM72_03785"
     CDS             12901..14457
                     /locus_tag="PKM72_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNP59935.1"
                     /translation="MKRISPRWRSFAAHLRGNQSDAEQRLWEEIRDGRIKGWKFRRQH
                     PIGRSIVDFCCLERMLIIELDDGEPQEQETADEESTKYLTALGFRVLRFLNDDVLAKP
                     ENVVEEIVRVMGNFENLPSPPTSVIFSEPSPDRKMEPEDSHTHTFSNQAKVPHTGRRS
                     ALGFRMPAEWEPHEATWLGWPHNTKDWPGKMGAISWVYGEMVRKLSLGEDVRILVNDK
                     DHELKARRVLDKVGVDMTRIQFFRIPTNRGWARDFGPIFVKRDQPGEQVAITRFRFTA
                     WAKYPDWQLDDRIPVRLGPKLKIPVVPVERNRKGVVLEGGAIDVNGQGTLLTTEECLL
                     DERVQVRNPGYSRQDYEEMFGTHLGISQVLWLGQGIAGDDTHGHVDDVCRFVDPYTVV
                     LCQESNPTDENYRPLAENAERLEGMRVEDGRKLLVVPLPMPEPLYFEGRRLPASYANF
                     YIGNAVVLVPTFNDPQDRVALGIVADLFPDRTVVGIHAVDLVWGFGTIHCLTQQQPAS
                     DQLSILKERS"
     gene            14454..15413
                     /locus_tag="PKM72_03790"
     CDS             14454..15413
                     /locus_tag="PKM72_03790"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNP59936.1"
                     /translation="MNTSSPADDPIILPSPVRIGWIGTGVMGAPMCEHLLRAGYALTV
                     HTRTPSKAQILLDQGARWTESPAALVAEADVVCTMVGFPQDVRRIYLQPDGLLSQART
                     GQIFVDFTTSEPTLAGELASQAGSKSASALDAPVSGGDVGARDGTLSIMVGGDSRVFE
                     AVKPILSVFGRTLVYQGQAGCGQHAKLCNQITIAGTMIGVCEALLYAHKAGLDGETLL
                     RSISSGAAGCWTLDHLAPRILKRDFSAGFFVEHFIKDMGMALDEAKRMGLLLTGLSLV
                     RKLYVAIQANGHGRSGTHALLLALEDLSPTFITADSSPRSSSE"
     gene            complement(15511..15843)
                     /locus_tag="PKM72_03795"
     CDS             complement(15511..15843)
                     /locus_tag="PKM72_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59937.1"
                     /translation="MEKIRQQPPTRTWRLLCLIGLTLSVAFILNEGQARTLPMPHSTQ
                     SINGQSTHPTHMASKIHPLEKDHASQSTKPTTHHDRGTRERDNEEKPQKKKFGLAILF
                     LGTLTEKS"
     gene            complement(16052..17329)
                     /locus_tag="PKM72_03800"
     CDS             complement(16052..17329)
                     /locus_tag="PKM72_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNP59938.1"
                     /translation="MAIKRIPTTSVQVGMYLCGIDRSWLNTPFLVHRFLIKDSSDIAK
                     LQQAGIQEITIDTNRGHDVASDSEPSQTQEDEVSSKQGPPIRDSSLPPKTDRLQLLSP
                     SIQGKSLSGELTTMKLVRKYMLEGVQDILLTLQKTGGLAIEQVHQITESVMAETLGHE
                     EACIAMIRTRAFSPALYDHSLAVSTLCVLLGRAVGLDNTVLQHLASAGLLHDAGLLNL
                     PQVLHRPLNQLSDSELGLYQKHPKRGVEMLSQQTCLSDEVDTLIREHHIELDGSGFPA
                     NIDPTKIHTPTRILRIVDEYDELLTGHHTGNPLPVRIALQTLYQQGRKGLLDGELVAT
                     FINLIGIYPTYALVELSTGERGIVTANSRGNLLQPTILLIQDEIHRPLPEPIPFNFSV
                     LSSHELRPEIVRVLAPEEVGIDLDSALENWMTL"
     gene            17632..18426
                     /locus_tag="PKM72_03805"
     CDS             17632..18426
                     /locus_tag="PKM72_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59939.1"
                     /translation="MSGCRTSFQSEWADSLTVGPASPQDVSSTVWLFLGGVLLSIFLS
                     LGFSSSSYAASVTDFPIKPVRRAIPILGTTTNAQSEQVGIVAEVQLEFVQRRDHKGLD
                     LQFLNRPGKFSPYAQQSVKDALILVVQAAGLNPDSWTVRFMLPYPGVTLYGESLSAMA
                     ALNVVALAKNDPVDEKTVLTGTVTMDGHVGTVGGVPLKILAAHEQRYRRVLIPEEPDV
                     ADDDWETPFLMEVSPVGSIKHAYLALTHRPLRSHLNEQPFAATLSQ"
     gene            18645..19313
                     /locus_tag="PKM72_03810"
     CDS             18645..19313
                     /locus_tag="PKM72_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59940.1"
                     /translation="MIRPCFAVDIDNVLGRAEPEVQRLFQELTGRLWPVGLYGSAGGL
                     DASQLAPELLEEIFSRFHQESIPRLPLFPGAKLALTLIHRRYRIIIVTARRPYSRPQT
                     LRWLEEHGLPFDALYHTEEKTDIPESITAAIDDHPHHIQAYRALDRQVFVMDQPWNRG
                     IPHADVIRVTGWDALLQWLHFRQLQQSPKKFPETPSFRHLFSSHLNVSSLTAVGVSTG
                     SAKP"
     gene            19443..19928
                     /locus_tag="PKM72_03815"
     CDS             19443..19928
                     /locus_tag="PKM72_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59941.1"
                     /translation="MMTLPIHTVLLVDDNPDDCEIVREAWEEITVGQELRCVNDGTEL
                     LDYLYRRGLFSGRECAPRPSVILLDLNMPHMTGGEVLIEIKKNPSLACIPIVVLTTSI
                     APKDICSTAGMGVNGYMQKPNSYTGYIQMLVNLRKHWAEVLKQPLTGRGGDCSSNVAW
                     C"
     gene            20380..21246
                     /locus_tag="PKM72_03820"
     CDS             20380..21246
                     /locus_tag="PKM72_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59942.1"
                     /translation="MKTLFGLTGAVVMLGTSLLTTSLAFEASEYLTLTRHRDSNGHLL
                     AGPTHHQPQPSVIQKTVVPAQPQTFERRSTEWGMNAQEVKLNEPVQPVWELQSPILEP
                     YEQRVGYRRQIEEIDASLTYTFYEDQLAQANYLFEPKHDDAVDYVQDFHTVKDWITQS
                     YGMPTSFQEIWLDSLYQYDQSLWGQALLRGHLKMVAEWRNETTNITLILDGGEDTIGL
                     MADFDSVAIIAPAPMEARIIEGNPIVEDVSIGESSVRDEHSLDAPPMEALPVETMPTE
                     SISPEQYPDTKL"
     gene            complement(21466..22074)
                     /locus_tag="PKM72_03825"
     CDS             complement(21466..22074)
                     /locus_tag="PKM72_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNP59943.1"
                     /translation="MTYYRTIRLSRVIFRYKLVFLFSLAAICAGCSTSYKNSLPPDLA
                     EHIEPGLMFPRIKNDPDSFKGKLVILGGQILAAKRLEDRTQLTILQLPLINEEEPTTE
                     LTQSEGRFIAEQQKFLDPATVPPGSRITLAGELSGSVQRSLDEDIYTYPTLLIKHFKI
                     WPAYSSEYHRYGPYPSPFFYPYAYPYWGGYGRFYPYYPFGYW"
     gene            complement(22550..22873)
                     /locus_tag="PKM72_03830"
     CDS             complement(22550..22873)
                     /locus_tag="PKM72_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59944.1"
                     /translation="MKYFSILAILCSMFLTAPAWAHGDAAHVMGTVTESGNDHVVVTT
                     PKGETLTLAFHPKITFQQNGIHVEHARPQVGDRLVAEVSKKGVPENRDWVATEITFST
                     PKKKP"
     gene            complement(22899..23504)
                     /locus_tag="PKM72_03835"
     CDS             complement(22899..23504)
                     /locus_tag="PKM72_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP59945.1"
                     /translation="MFKRIKIFFIIVLLFLVGSTLTALWLLPGAFSAKSTPPEWEVKA
                     ARFIRHLATPSHFLKMTNPVPHSSEALGEARHHFADHCATCHANDGSGKTHMGPNFYP
                     PVPDLRDTAIQAMADGELFYVIHYGIRFTGMPAWGKGPPEEDLDSWKLVHLIRHFPDI
                     TPEEIAEMKQYNPLTRAEREQQEALDQFLSGEDVVPQEHHH"
     gene            23706..25283
                     /locus_tag="PKM72_03840"
     CDS             23706..25283
                     /locus_tag="PKM72_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP59946.1"
                     /translation="MTSSPLIKSLKKRECYSHPVSGVTLVETHISWVFLTGQYVYKIK
                     KPVLFSFVDFSTLEKRRWFCEEEVRLNRRLAPQLYLGVVPITGSPSNPRIDGDGNPFE
                     WAVKMKQFPPDQEFHKLLETGNLDESVISQLAIDIGTFHGRIDSSGDTFPYGKPDMVW
                     RPIAECFEDIPLAALPERIQFCMKDIKEWTNREWTRLRPLLRGRKSSGQIRECHGDLH
                     LGNIALFEGRTCVFDALEFKPGLRWIDVISEVAFLVMDLESKDRVDLAYWFLNCYLEV
                     TGDYAGMSVFRLYEVYRALVRAKVAGLRLAQVESGSPVGEDFLEEIIRYVELANRLVM
                     SVPPMLMLMHGVSGTGKTTVSTEVVKALGAVRVRSDVERKRIYAEQFKKVVDQGASGS
                     LYAPAMTHATYDRLWDLANGMLQAGYPVVVDATFLDGAQRQSFFRLAHERHVSVVILD
                     VWAPDEVLAERIACRAKQRTDASDATIRVMEQQRTKEDPLTELEEDTAIRLDSTDLPT
                     VRSTIESLIKQRRKYAD"
     gene            complement(25398..25973)
                     /locus_tag="PKM72_03845"
     CDS             complement(25398..25973)
                     /locus_tag="PKM72_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNP59947.1"
                     /translation="MMASLLRTVRNERGTASIELAFSLMIFLFVAFGIVEYGSIINER
                     NALTQLAREGASLASRNLTTNQNMMDLLGSTDNALDFKNHPSKYHIYLAQITAGTVAN
                     NNPTCTVVEQGGFNGAGVSPPALPNCDLPQNLYDYLVYVPGTGATVQQFTVVKVYYEH
                     DPMTPVGALGAQFGWGGNLGNSVTLFSRAIF"
     gene            complement(25970..26503)
                     /locus_tag="PKM72_03850"
     CDS             complement(25970..26503)
                     /locus_tag="PKM72_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNP59948.1"
                     /translation="MNSLIRFFRKLMSPCQTESGISAVEVGATMLVFLTALFAVAEMG
                     WFFVHQNTLTSAVREGIRIGTVGATSVDGNGNALSRENSIKQAIQQSASAVMDIDSSD
                     IFIFPVDADWTDPDDPAVVATASAGGAGAFMRVRVNFTHQFFTQLIGGLFGPGDTIVI
                     SSEGTYRNENFILGGGS"
     gene            complement(26581..>26786)
                     /locus_tag="PKM72_03855"
     CDS             complement(26581..>26786)
                     /locus_tag="PKM72_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNP59949.1"
                     /translation="KQMAIDNAAAIKAQGIEIYTIGLGSVDPAFLGTLSSGTAFAYYT
                     PDSSELEGIFQQIANILKLVLIS"
BASE COUNT         6388 a         5931 c         7197 g         7170 t
ORIGIN      
        1 taaacttgga gctacgtgct ggcttaagcc gggaacaagg gcttcggaat ttggtgaaca
       61 ggacgaatct tgacgatatt cgtagcttag tcgccttgct gattcagacc gatcgatttg
      121 gtacgagtat tgggcaggcg cttcgagtcc attcggattc catgcgtttg aagcgaaggc
      181 ttaaagctga agagcggggg gcacagcttc cagtaaaact gatgatccct cttatattgt
      241 ttatttttcc agcattaatg gtggtgatta ttggtccggg tgctataagc cttttgcgaa
      301 atttaatgcc ggctatggga gggtgaacgc ataaaagagc cacgaacgat agattcgata
      361 aacttttcaa aagcgtagag gaagacataa catgggagtt tcattttaca aattaacaga
      421 acaagacatt ccaacagcga atatgactgt ttggtcgatt tcgttactgg cgataaaaga
      481 aacctttcag atgaggtaca caatgatgaa ggcgagcctc ctcgtaactt tggttgtatt
      541 gttcagtttg ggtgtcgttg ggtgtgccaa aaaagaacag tccatgttca cgctacagga
      601 tgaacattac cagcaaatca tggaacggca gaaggcggga atcgattcgg aatctcaagc
      661 gcctgtagat ctcactcaag tcatgaaggg tgaggagtat gaaaaactgg gagatgccca
      721 tttacagcaa ggcgatttta ggacggcaaa aattcaatat gaaaaagtgt tagaggcttc
      781 ccctgaacag attcctgcgc gatacaagct ggccgtgatc tatttggaaa aaggatcccc
      841 tcaggaagcc tataaccaat ttcaccaaat tttggattat gatttgaatt ttgctccagc
      901 ttacgagggg atgggtcgag ccttgctgaa gatggagaag gatcaggagg cagagcggga
      961 gttccaagcg gcactattgt atgacggtga aatgtggacc tcccaaaatt accttggcat
     1021 tgtggccgat cggcgtcatg atcataaaga ggcgatccac ctgtatgaaa ttaccttgca
     1081 gaaaagggca gatgatccat cgatcttgaa taatttgggt atggcctatt acttggataa
     1141 ccaatatgaa gaggcggtca ggacctttca acatgcgatt caggttggtg gatccaacga
     1201 taaaatttcg aataatctag gattagctct caccaaactt gggcatttcg atctagccta
     1261 cgacgcctat gctcgaggaa tggatccagc caaggcctat aataacttgg gtgtggcatt
     1321 tttggagatt gggaagtttg cgcgagcttc ccgttgtttt gagaaagcca ttgaaaccca
     1381 accgaccttt tatgaaaagg ccaaagagaa tctgttgctg agcaatcgta tgctgtcgaa
     1441 attccccatg gttcagcagc aaacattact ccgacgggac cccgtgtgtg tgtcctgagc
     1501 aattatgaaa aattccctgg atggacgcat tgtgtcaatg ccttctgccc agatttgatt
     1561 ggtccgcttg agtcattcct gacagagatg atcgggagtt cattggttgt gtaataaatg
     1621 ggcgtcttct gacaatcatt tagaaaaatc cttacctgat ttttcgttga cttaccttca
     1681 tgtagaattg ccaccgtggg gaaggaaccg tcctctcgtg gaccttccct tcgtcaagct
     1741 cacccaatat atgaatttca atgattggca attctcgcgt tgtgcgagct tgtgggtgag
     1801 gcggtcgggt aagccgtatg ttttcctagt ctgttccttt tttgaatttg tccgtttcct
     1861 aaattgtttt cgatacggac gatggttatt ccctgcttct caattatcct atcatgcatc
     1921 caatatcatc ttttccttta aaatgactct cggtgagact gcggtagaga acaacaggaa
     1981 atctatcggc taagccgaat acgctggact ttgaaattaa atattccctg ctctttgttc
     2041 attctgtgtg acggtttttt ctgagtacga gggtcgagta gtctgtcttt cagagataat
     2101 actcgagtca gagaccccag ctattacgat gaggatctcg agcacgtcag tgggtcgtct
     2161 cacccttgtt ttgagaaaac atgcattcaa gagtttttgc caggtcgaat attttataag
     2221 gcttgaccaa aatcccttgg aatccatgtt cttgaaaatc gcacataatg ggatcgtctg
     2281 aatatccgct ggtgacaata gctttaatgc gagggtctaa ttttctaaga tgctgaatgg
     2341 ctttcacccc tcccatggcg cctggaatgg tgagatcgag gatgaccact tcaaattttt
     2401 ttccatccgc cagtcctttg gaaacaaggt tgatggccgt ttgcccatct ggcgcgctga
     2461 ccacatcata gccgaactgc gttagcgcat cctcaaccat acggcgaatg ctgtgttcgt
     2521 catccataac caggacccgt cctctataat ttcggggaat ggctggaatg ttctctgtct
     2581 caacttctgg cgtgacatgt gcggatggta gaaacagggt gaaaattgtg ccgactccga
     2641 cttttgatgt cacgctgatg tgtccatgat gttgctggat aatgctatgg gcggtggcta
     2701 atcccaaccc ggaagcgcct ggtttggtgg tgtaatacgg atcaaaaatg tttgccagtt
     2761 ggcgtgcctc aatcccgttc ccctggtcct caaaagaaat tcgcacatag tttccaggga
     2821 tgagtgccgg cgaaggaagg gccgaggaat cattcagtct cacgttttct actttgaggc
     2881 tcaagtgacc agcgtgaggc atggcctgtc ttgcattgag agtaatgttt tgaaagacct
     2941 gccggatttg accgggatca gcatcaagag gccaaaggtc atcggggatg tcgaaatgac
     3001 aactgattga tgaccctgat agggcaaaaa tcgtgctttt tcgaagtaat tctcctaacg
     3061 cgatggatgt ctttatcggc gctccgccct tggcaaaggt cagaagttgt tgggtcaatt
     3121 ctttggcgcg caagcaggct tgctcggctt gctcaagatt ttgagtcaag gggtcgctcg
     3181 agaccattcg caatttggcc acaaacacgt tgcccaatat cgtcgtgagg atattattga
     3241 aatcgtgagc cagccctccc gccaagaccc ccaaggaatt gagtttgctg attcgttgtt
     3301 gttcttccag ttgacgggat tggtcggtga tatcccggaa gacaatcacg gtgcccacga
     3361 ggtgaccact atgatcgcgg atcggtcctt ctctaacggt aatcttccgt tgccggccac
     3421 ctttggtcag taattgctga ggggcatcca gctcagaaag gacaagtgtt ctggaagaaa
     3481 actgtaaaga cggctcagca tcgttttttt tgctatcacc ggtcagacgg aatatttctt
     3541 ggaatggccg gtgcaaggcc tcatgggttg tccattcggt gatctgctcg gccaacgggt
     3601 ttaagaaaga aatccgtcct tctcgatcag tcgtgatgat gccttccgca accgattgaa
     3661 gcgtggtgac cagatgatcc ttttcagcaa tgagggcctg ctcgaggcgt ttccggtcgg
     3721 aaatatctcg aaggataaag ccgtgatgtg tgtccatgcc gactttccaa ctcgtggaag
     3781 aaatttccaa gggaaaagat tcgccgcttt tccgtaggcc ggtgagctcc aatggctgct
     3841 gaaggggaaa ggttgaaggt cgaccataac tgatatctcc cagtcctttc ctgtacatgt
     3901 ctcgatatcc ttcttccagt aaagtcacaa tcgaatgccc caggacctcg tcaggctgat
     3961 atccgaacat tgactgggct gcaggattcc aagagacaat ggttccgtgt aaatcggcgg
     4021 agatgattcc ttcatgggcg gagtccacca gagaggaaaa ttgagaatgg agttcggtca
     4081 tggtctggct gacccgcaca tgatgcgtaa tatccaggcc atatccccgc acgagacctt
     4141 tttcctggag aggggagaag gtccaatcaa agtggtggcc ctcgacaatg accgtgagac
     4201 ctctgatggg ggttatcgtt ttgaggcatt ctagcgtgag ctgtgtcagg gtgcttggaa
     4261 gcaccattgg cacgccgtct tcccgaaatc cataggtgtc gaggaggcgg atcatcgctg
     4321 ggttggcata ggccaggtgt ccttgagcgt ccacttccac gatgggattt gggctttctt
     4381 ctggcatcga cgcaaggtgg tgatgccttt cgtgttgctg gagttccagg ctgagatcac
     4441 gaaaaataat cagcgcacca gagtggcgcg gatctgtcag tggcgtggat tggaattcca
     4501 cccatcgggt ttccccactt ggccgtcgaa tcaataattg aggcgtccat atggattttc
     4561 ccgtggtgat cgctctggtg atcgggcaat gttcttcttg tgtgccaaga tcaatataga
     4621 gacattccgc atatgcatgg aaagattgtc ccaggcatga ggtttcggtt cgatgaagaa
     4681 tcctggcgcc ttccaggttt agggaaagaa tctgtccttg tgtattcacg tggcagatgc
     4741 caatgtggat ggagtccatc agggtatgaa caaaggcttg ggatgaacgt aatgccttct
     4801 ctagtcctga aatgtccatt tgattgagtt cttgaaaaaa atgttgccga tccactcagc
     4861 gattccttcc tgtcatattg atgacattgt gtggacgata ccgagtctta ccataaggct
     4921 tcaagaattt ttgacatatc ccatccttcc cgagaagggt ccagggcttt ttcaattgat
     4981 cgaaaaggtg ttgttgggct ttgtgccgcc agatcaatga ttgcctgctc gggatagggt
     5041 tggtccaggg agtccttaag taatttaata gtaggggtca ttcggctatg actagaaacc
     5101 cacacgacta cggcctgttc gccggtattc aggcgaacca gggaaccaat tggatataca
     5161 cctaagagtt ggattaattg ttgtacaaga tacagatggt attgcccttc gacagcagcc
     5221 tgatagagtt cggataaggc ccgggcgggc ggcacaggtg gacagctgca tcgtccacta
     5281 atttgcgcgt catacgtgtc ggcaatgctc agtatttggg tgagttccga aatctgaagc
     5341 cctcgtaatc ccttggggta gcctgatccg tcaagccgtt catggtgttc caagaccatc
     5401 tgtttcgact cgtcagggat atccgggaat tgtttaagaa tagccacgcc tatttcgcaa
     5461 tgcgcgtgca tcagtttctg ctcttgcggt gtgaagcgct ccacctttcg gatcatattc
     5521 aatgggaggc gaagttgtcc gacgtcgtgg agaagggcgg ctagtccaac gagttttaat
     5581 tgcgtcggat cgcagccgtg ttcgtgtccg attgccatgg ctaacatgcc cgtatctacc
     5641 ccatgtgagg ccaggttggg atcgaagcga cgcatttgaa tcagcgacac catggcggcc
     5701 tgatggtcta aaatcccatc aagtaggatg ctgaccacac ttcggacttc ttggacgtgc
     5761 tggggagtgg gtgcttccat ctgatggaaa aatacctcca tggtggagat cgcttctgcg
     5821 cgaaggagac gcgcggcttc aatatcttcc ggattgggtc ctgagccaac cccaggggat
     5881 gttaacgggg ttgtgggaga aaggtcctga tcctctgagg tgtgctgacc gccactcgtc
     5941 ttggcaatat cgcatccttt ttcggtgtcg atctggacct ccttaattcc gtaattgcgc
     6001 aataaggcaa tttcatcctc tcgtttgatc gtccatttgt ggcggagaaa cggggtttta
     6061 aaccatgatc ggtccatgcc ggtgatatac atcccaggcc tcaggtcctg aatagaaata
     6121 cgtttaatca tggtgcaagg ttggacaaag ggggataaaa aacgcaccag atgagatctg
     6181 agatataaag ctgggtatca accagccggg ggctggatag aattgtgtca atggtaacca
     6241 tattcccgaa aaagggcaag aagatgccag ggccgagcca gcccgctaaa ggctggactg
     6301 aagtggttga cgcaagatcg aaacgaggag caattttgga gtaaaacatg ggaaggatta
     6361 ggaggagcgt ttgagaattc gattaatgcg gccggcaatt tccgtcgctt ccttctggtg
     6421 atctcccgcc aataggaggg catggtagtt ttgcagggtt gagagtaatt gcgggtccga
     6481 aagtccccag gacttttcac gaagggccag ggctcgttta agaagtgggc gggccatctc
     6541 taattggttt ttctgctgat acaacgaggc gagtgcggct aaaggatcgg ccatccgaag
     6601 gtgttcagca cccaacgatt gagccagcaa gggtaaggct tcccaataat aggcttcagc
     6661 cagactctct tctccttcta gccgggcgac ctctcccaac tgaaaaatta ccagcgcata
     6721 tcgttcatcg gtcggcccat gttcgcgaac ggatggtaac agaccatgaa gcaggtcttt
     6781 ggcttcttga aattgtccac gggtgatatt cgcatggacc tgctcccatt gccgatccca
     6841 ttcggaagat gtcgggcatc cggtaagcaa agaaatgagg cctgcaagca gccaccataa
     6901 aaataatgtt cggtgtgcgg ggtgtttcat aaatgtcgga gaggagtctt cacgcctgac
     6961 tggggagcca agtaagtgaa gaaccacgcg tgttaaaagt tgagagccat gatgggaaaa
     7021 tcctgtggtt ttctggacat tttctgtcac tctttgtagg gtatccaggt gcaaggcctg
     7081 tgacggatat gaagatgatc aaccaagtat tgcgatattg ccaggatgac gtgtggaagg
     7141 aggatgtcaa tgcgctaacc ggttttcgac agttcggcgt taagtttttg cgtctggcat
     7201 tggtggcggt cgccgaattt cagcaaagtg ccctgagtat tcgagccacc agtttggtct
     7261 acaccactct cctctccctg gttccgtttt tagcagtcac cttttccgtc ctcaaagcct
     7321 ttggcattca tcaaaaaatc caaccgattc tggcgcaagc gttggatccg cttgggccca
     7381 aaggagttga gattaccacg aatatcatcc agtttgtcga taatatgaaa gtcggggtct
     7441 tgggagctgt gggcattgcg ggactgttct atacgacgta ttcgctgatc gaaaaaattg
     7501 aagaggctct gaattccatt tggagggttg aaacaggtcg cccgttagcc agaagggtca
     7561 ccgattattt aagtgtcgta cttgtgggcc cggtgttggt ctttacggcc tttggactca
     7621 cggcttcggc tcaaagtcat tggcttgttc aaaaaattct ggcagtccag ccgctgggcc
     7681 atctgatggt tgtgctcact aatctgctgc ccttcttgtt tatgtgcctc gtgtttacat
     7741 ttatttacaa atttttgccc tatacaaacg tcaaattaaa ctcagccctt gttggaggag
     7801 tgacagccag tctgctctgg caagtggccg gattggcgtt tgcatcattt gtcgtaggga
     7861 caggacgata tagcgccatc tattccgggt ttgctgtgct tatcctcttt ctcatttggc
     7921 tctatgtcgg atggctcatt gtattggcgg gagcccaagt cgcctattat caccaacatc
     7981 catccgcgta tttgctgcac ttaagtcgaa agaaacagac tccacttttt cgagaatatc
     8041 tcacgctgtc attgctaacg catattactc gccggttttt attggggaag ccgcctctta
     8101 gggcagagca gttagcccgc gcgcaaggtg tgcctctgac cttggtggag tcgctcatcg
     8161 atgatctggt gaaagggcag atggtggtga aaggggatga tcccaaagga ttggtgttgg
     8221 cgcaggcacc ggaaaacatt caggttgtcg aggtgttacg agtggtgcag catcaacccc
     8281 atagcggatc ccaagctttt acagtgggat ccgataatat tagtcgggtg ctcatgcacc
     8341 gggatgcggc ggtggaggcc gccttagaag gcatgacatt gcgggattta ttgcgacgtc
     8401 aatctgaaat cgttgaagaa gaggtcagaa ccgaccatcc cgagacccaa ggttctggtg
     8461 gagcttttca ccctgagtcg gagtgtgggc attctgtttc tccagactat ctccaagaaa
     8521 ccagacagac tgatccttct gttgttggaa aagggccggc acggtaatca gcgtatgctg
     8581 aaaaggggaa gtagatgaat atgtggattg tcccaggagc gccgttgaag gagggccaag
     8641 gttggcggat ttggtgttcg caaaaaggtg aggggagttt ttctccgccg caagtcgagg
     8701 tgcggcagaa gaatgcgacg gtcccctccc ggcatacatg gacgctactc ccccaacttc
     8761 caggattgaa gaggcgaatg ggtgtgatgg ttctcacgct cagcacttcg ggtccgccgg
     8821 aaggcgcact ctattcggtc atcctgtctg caaatcaaca aaaccgggtc tttcaatggt
     8881 ggacaatgcc gtaccatctc gctgctcagc cggtaacctt tctgtttgcc tcctgctttt
     8941 ggcataacaa tgatcgtgag gggtattacc ggtccggcct tcaggagttg ggacagttgg
     9001 ctcatccgca ctttaaatta ttgctgggtg atcaggtgta tggcgactgg ccgaatgcgt
     9061 gggatttagg cgatgaggga attgaactct atgcgaagcg atacgctcaa tattggggag
     9121 atgaggccta ccgggaagcc ctacaagtct gcccgaactt ttttacctgt gatgaccacg
     9181 aatattggaa cgattatcct gaaaaacaaa tccaactccc gcagacctgg gcgtcgaaga
     9241 atcgcaaaac ctatggggga ggggctgagg acctctacta ccagtatcaa cgatgtttga
     9301 atcctgatga ggctcgctgg taccggtttg agatcgatcc ggtctcgttt tttattaccg
     9361 acacccgttc agagcgcgaa aagtgcaacg acaaagggac cagtcatttt atgtcaaagg
     9421 agcaatgggt ggcactggaa gcctggcaac agggattaac gggacccgga gtattggcgc
     9481 tgggccaacc gctttttcaa aaagacgggg atttccgtga ccattccttg tcgaatttca
     9541 agcaggatta cgcccgtctc tggagggtaa tcgaacggtc cttagccggt cagaattccc
     9601 agcatcaacc acacgatatc ttgattctgt ctggggatat tcatacggga cgttatgctg
     9661 aagcccatgg gccatttcct gatgccccct atggggtgcc ggaattcatt gcctcgccgg
     9721 cagccatgat caatccagga aattccagac ccgaagcccc gccagaaaag atccggattc
     9781 ttcctaatgc ggaaggaaag gagtcggtgt ggcggatcga tcctcattcg aacattgata
     9841 tcatgaccat tcagaataat gtgggtctcg tgcgtatggt tcccggaagt cagaccggaa
     9901 agtcgccacg agtacgattc gagttagaat tatggagtct gccttccaaa gttattgaaa
     9961 ttgattggga tgaaaccgcc ccacctcaag gtcttggtgg tcccttgaag cgcctctgga
    10021 agaaggagtt gttgctccgg taatggatta ttcggctaga tctgccatgg gtgccagcct
    10081 atgtcctgtg tgcgttttct aagcctcctc tagaaaggaa ctcatcatgc gccattctca
    10141 aacttcgacc agaaatccgg acattaaaga ctggaccagc aaatatggaa aacgcactaa
    10201 tctacaattt tttaatgatg cgtttgcgta cttgcagaaa caggatattg gcaatcgtgg
    10261 attgatgact gtagggttga tcggcagccg ggatgttccc gggcatactc ttcgaactgc
    10321 ccagtctacg gtgcgttggg atcttcgccc gagtttctgg tcacatgtct ttgtggtggc
    10381 ggaaccggtg acctctcgga cctcgctgag atccctgccg atcctggaaa tcccgctgca
    10441 tccacggaat gggatcttcc caaggccgga atgtaatggc atcaacgaag gcaccttagg
    10501 cctgtatgag gataaggatg ttgatgccaa tgtggggttg gtggcggtaa gcatgtcaga
    10561 cgaggatgcc aagaaactta agaagcgggc gatgaactgg aatcaggatc gtgtacgtta
    10621 caatttttgg gagatgttgg gagtatggca aagctatctc tggtcccagg gggccaaacg
    10681 caatccgctg cgggaaggca cgccgatcgc cgcgtcctcc tatattgaat tcatctttga
    10741 aggactcagc ttgggcgtga cgccgggtac ctcggaacgt aatagcgccc cggagcatct
    10801 ctggaatgcg gcctgctggt ggcacaaggc cttcaaggag caagaccgca aagtggccgg
    10861 ggtgtttgtc gctcgagatc gcgggtgtgc catggccggt tgcgacgann nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gccggggtgt ttgtcgctcg agatcgcggg
    11041 tgtgccatgg ccggttgcga cgagtaggtg caacttgcat ggggacgtaa gagtgttgcc
    11101 atggcaggta ttccgttatc cctgaagtag gggtatccat ctaaaaagaa tgaggataga
    11161 gtcccgagga acgatgttgg gaatgacacc gtgttttctg gtatctcgaa gcgcggtcgc
    11221 cacttttacc ggagaaagtc ctattgtgtg ttgtgccgtt tctcccgacg gccgaacgat
    11281 tgtgattgga gaatcctccg ggcgggtgca tgttttacgt ttagagggta ttgattgaag
    11341 aattttttgc ttgtcctgtg catcgcaatc cactaggctt cgttttcttt cttgcccttt
    11401 taaggggagg atcaaggtgg ggtagaatcg ccagatgcct tatgaaccgt gatagggcgc
    11461 tgtacaagtg tcgactgtaa ctcgggagta tttctacctc cccttttatt cctcttcgga
    11521 attaaacctc ctaacaaagg agtgggacat aaaatacggg taaaaatatg atgaaaatcg
    11581 gattgattca aatggcctgt tcggccaatt ctgctgagaa ccttgaacgg gcagtggcta
    11641 aagctgaaga agccgccaag ggcggggcgc agattatctg tctgcctgag ttgtttcgct
    11701 cccaatactt ttgccagaaa gaggatgccg atctttttga tttggcagaa tccgttcctg
    11761 ggccttccac agaagccttt gccaagattg ccaaatccca tcgcgtgacc attttagtat
    11821 cggtgtttga gcggcggacg gcaggggtgt accacaattc cattgccgtg ttggacgagc
    11881 ggggagagat agctggcctg tatcggaaga tgcacatacc cgatgacccg gcctattacg
    11941 aaaagtttta tttcacccca ggggatcgtg ggtttcaggc gatcaacacc acgcacgcaa
    12001 gggtcgggac gttgatctgt tgggatcagt ggtaccccga agcggctagg atgacggccc
    12061 tacagggagc ggagatctta ttttacccta cggccattgg ttggcatccc aaagaaaaat
    12121 cagaagaagg ccaggcccaa cgtgaagcct ggatgacaat tcaacgcagc catgccatcg
    12181 ccaacggggt gtttgtggtg gcggtgaatc gggtcggcca tgaggctccg gccgggggtg
    12241 atgggttgga gttttggggc agttcgtttg tgtgtgatcc gtttggtgtg gtgctggctc
    12301 aagcgtcatt tgagaaagaa gaaaccctgg ttgtggatat cgatctgaaa cgcattgagg
    12361 aagtcagacg taactggcca ttccttcgcg atcgccgcat tgatgcgtat ggggggatta
    12421 caaagagatt attggatgaa tagcataggg cctattttac tggtcctcgg taagatctga
    12481 tctgtcctcc ctttccctcg cgctttttgt tgaaaaaata aggccccttg ttgggagagg
    12541 gaacacattg tttctccaca aattttccga tacaacatag ttttttttac attaaaatct
    12601 atacatggga gtgctgaata ataatagttt cctagggcaa attagcccac aggtacgttg
    12661 catgtcagag gcctcggggc ggttcaaaaa atttcgtcca gcaaggccgc agtcattttt
    12721 acgcgcagag cgtacttcca gtacgtgagc acggaaacat ggcgagaacg ccgctggcgg
    12781 tttttttcaa cagccccata catgtatgct gtgcttcgcc tattcataag gctagatgat
    12841 tcggttgccc cattgtgatg tggttccgaa gaggaactga aggaagagtg aaggggagag
    12901 ttgaagcgaa tttcaccgag gtggcgatcc tttgcggctc atttacgtgg gaatcaaagc
    12961 gatgctgaac agcggctctg ggaagaaatt cgagatggtc ggatcaaggg atggaaattc
    13021 cggcggcaac atccaatcgg taggagtatc gttgattttt gttgtttgga aaggatgctg
    13081 atcattgagc tggatgatgg agaaccacaa gaacaagaaa ccgctgatga ggagtccacc
    13141 aagtatttaa cggctttagg gtttcgtgtc ctgcgatttt tgaatgatga tgttctggct
    13201 aagcctgaga atgtggtgga ggagatagtg agagtcatgg gaaattttga gaaccttcct
    13261 tctcccccta cctctgtcat tttttcggag ccgagccctg acaggaagat ggaaccagag
    13321 gattcccata ctcacacttt ctctaatcag gcaaaagtcc cgcatacggg gaggagatct
    13381 gcattagggt ttcggatgcc cgcagagtgg gagccacacg aggcgacttg gctaggatgg
    13441 ccgcataata caaaagattg gccggggaaa atgggagcca tctcctgggt gtatggggaa
    13501 atggttcgga agctttcttt gggagaagat gttcgaattc tcgtgaatga caaagaccat
    13561 gaattaaaag ccaggcgggt gttggacaaa gttggcgtcg atatgacccg tatccagttt
    13621 tttcgtattc cgaccaaccg gggatgggcg cgggatttcg gccccatatt tgtgaaacgg
    13681 gatcagcctg gcgaacaggt ggcgattacc cggtttcgtt ttacggcatg ggccaagtat
    13741 ccggattggc aattggatga tcgcatcccc gttcggttag gcccgaaatt gaagattccc
    13801 gtggttccgg ttgagcgaaa tcggaaagga gttgttctgg agggtggcgc catcgatgtg
    13861 aacggccaag ggactttgtt gaccacggaa gaatgtttgt tggatgagcg ggtacaggta
    13921 cgtaatcccg ggtattcccg acaggattat gaggaaatgt ttggcaccca tttaggcatt
    13981 tctcaggttc tctggttggg acagggcatt gccggtgacg atacgcatgg gcatgtggat
    14041 gatgtgtgcc gatttgtgga tccttatacg gtggtgctgt gtcaggaatc caatccaacc
    14101 gatgagaact atcgtccatt ggctgaaaat gccgagcgat tagaggggat gcgggtggaa
    14161 gacgggagaa agttgctggt ggtgcccttg ccgatgcctg agccgcttta ctttgaagga
    14221 cgacgcttgc ctgccagtta tgccaatttt tatatcggca atgctgtcgt gttagtgccc
    14281 acctttaatg atccgcagga tcgggtggcg ttagggattg tggctgacct gtttcctgat
    14341 cgaaccgtgg tcggtattca tgcggtggat ttggtctggg ggtttgggac cattcattgt
    14401 ttgacgcaac aacagccagc ctctgatcaa ttgtcgattc tcaaggagcg ttcatgaata
    14461 cctcatcccc tgcggacgat cctattattc ttccctcgcc tgttcgaatt ggatggattg
    14521 gcactggagt catgggcgca ccaatgtgcg aacatctcct tcgtgcgggg tatgccttga
    14581 cggtacatac ccgcactcct tcaaaagctc agatattgtt ggaccaaggg gcccggtgga
    14641 cagagtctcc ggcagcctta gtcgcggagg ccgatgtggt gtgtacgatg gtgggctttc
    14701 ctcaggacgt tcgtcggata tatctccaac cagatggctt gttgtcacag gctcgtacgg
    14761 gtcagatctt tgtggatttc accacttctg agccgacgct ggccggtgaa ttagccagtc
    14821 aggccggttc gaaatctgcg tcggctcttg atgcgccggt ttctggcggt gacgttgggg
    14881 ccagagatgg tacgttgtcc attatggtgg gtggagattc cagggtattt gaagcggtca
    14941 aacccatttt atccgtattt ggaagaactc tggtgtatca aggccaggcc ggatgtggac
    15001 agcatgccaa gctctgtaat caaataacga ttgccggcac gatgatcggt gtgtgtgaag
    15061 cgttactcta tgcccacaaa gccgggttgg atggtgagac cttgttacgc tccatatcca
    15121 gtggagccgc gggatgttgg acacttgacc atctggcgcc acggatattg aaacgggatt
    15181 tttcggccgg attttttgtg gagcatttca ttaaagatat gggtatggcg ttagatgaag
    15241 ccaaacgaat gggcttactt ctgacaggac tgtcacttgt tcgaaagctt tatgtcgcga
    15301 ttcaagccaa tggtcacggg cgttccggta cccatgctct gttgttggcg ctagaagatt
    15361 tgtcgccgac ctttatcacc gcagattcct cccctcgctc ttcgtctgag taattcaaat
    15421 tcggacctga atttccccaa ttttttgcct ctgtgagaat ctgccttgct ccaatttttg
    15481 gagagtgttc tatctttaac aaaaagggaa ttagctcttt tctgtcaagg ttccgagaaa
    15541 taaaatcgct aacccgaatt ttttcttttg tggtttttcc tcgttatccc tttcccgggt
    15601 tcctctatca tggtgggtgg tgggttttgt cgattgggaa gcatgatctt tttctaaagg
    15661 atggatcttt gatgccatgt gtgtggggtg agtactttgc ccgttgatgg attgagtgga
    15721 gtgaggcatg ggcaatgtcc tggcctgtcc ttcattcagg atgaatgcga ccgagagggt
    15781 caggcctatg agacagagaa gtcgccatgt tcttgttggc ggttgctgtc ggattttttc
    15841 cattcttgag ccctccgttc cgcctctggt gcggtgcggg ttggttggga aatgcctggg
    15901 cctgtatcgg cattccgttt ttaaaccttg cgggcggact cagtggtgct tttttcttaa
    15961 aattgctgtg aatttcggtt cggcgttcgg aaacggatca atcccgagtg gagcgagaaa
    16021 gtcttcgcgg tctgaccgag aggagagatg gctacaacgt catccaattt tccagagcag
    16081 agtcaaggtc aatgccgact tcttccggag cgaggacccg cacaatttct ggccgtagtt
    16141 catgcgagga gagaacggag aaattgaacg ggatcggttc aggcaagggg cgatgaattt
    16201 cgtcctggat aaggaggatt gtaggttgaa gcagattacc tcttgaattc gctgtgacga
    16261 ttcccctttc gccggtactt aattctacca gggcataggt gggataaatt ccgataagat
    16321 taatgaaggt cgccactaac tcgccatcta acagcccttt cctgccttgt tgatacaacg
    16381 tttggagtgc gattctgacc ggaagaggat tccctgtgtg gtgaccagtc aataattcat
    16441 catactcgtc gactattcga aggattctgg tgggtgtgtg gatttttgtt ggatcaatat
    16501 tcgcaggaaa tcctgagcca tcaagttcga tatgatgttc gcggattaat gtatcgacct
    16561 cgtcagacag acaagtctgt tgggacaaca tttcaactcc ccgtttggga tgtttctggt
    16621 acaggcctaa ttctgaatca gataactggt tgagcggccg atgaaggact tgtgggaggt
    16681 tcaagagccc cgcgtcatgg aggaggcccg cgcttgccag gtgttgtaat actgtattgt
    16741 ccaatcccac agctcgtccc agtaacacgc ataatgtact gaccgcgagt gaatgatcgt
    16801 acaaggccgg agaaaaggct cttgtccgaa tcatggcaat gcaggcttct tcatgtccga
    16861 gggtttcagc cataacggat tcagtgattt gatgaacctg ttctattgcg agccctccgg
    16921 ttttttgtag tgtgaggagg atatcctgca cgccctcaag catatacttc cgcaccagct
    16981 tcatcgtggt gagttcaccg gatagtgatt ttccctgaat agagggtgag agcagctgaa
    17041 gacgatcagt tttcgggggt agggaggaat cacgtatcgg aggaccctgt ttagatgaga
    17101 cctcatcctc ttgagtctgt gacggttcag aatctgatgc cacatcatgt ccacgattag
    17161 tatcaatagt gatttcttga attccggcct gttgtaattt tgcaatatca cttgaatctt
    17221 taatgagaaa ccggtgcacg aggaagggag tattcaacca tgagcgatcg attccacaga
    17281 ggtacattcc cacttgaacc gatgttgttg gaattcgttt gatggccata cgtttgccga
    17341 tgagacgttt tagaatcgaa atctcattcc ctcaattgag gggaatgccc gatgtcttct
    17401 tcggtgttct ttcgcgcgtt cttaatcttc tccgtcattg ccggacaggt gatgagattg
    17461 aagcgctccc cctatcaaga atgtccttgt tgtccggaga aggaaacatc tatgaattga
    17521 ttaggcctag ggacgatcag agaccgcaag agatggtgcc tgccctcgta aagcttaagt
    17581 gtgctccttg ccaggccgaa aaggtgaaga cagctttcac gggggatggc gatgagtgga
    17641 tgtcgaacat catttcagag cgagtgggca gactccctga ctgttggccc tgcttctcca
    17701 caggatgtgt cctcgacagt ttggttattc ctcggaggcg tccttctctc catattcctg
    17761 tcattgggtt tttcttcctc cagctatgcg gcctcagtaa ccgattttcc gattaagcct
    17821 gttcgaaggg cgattcccat ccttgggacc acgacgaatg cacaatctga acaggtgggt
    17881 attgtcgcgg aagttcaatt ggagtttgtt cagcgacgag atcacaaagg attggatctg
    17941 caatttctta acaggcctgg gaagttttca ccctatgccc aacaatcggt caaagacgcc
    18001 ctcattctgg ttgttcaggc ggcgggactt aatcctgatt cctggacggt caggtttatg
    18061 ctcccctatc ccggggtcac actctatggg gaaagtttat cggccatggc ggcgttgaat
    18121 gtcgtggccc ttgccaaaaa cgatcccgta gatgagaaga cggttctaac ggggaccgtc
    18181 accatggatg gacatgtggg aactgtggga ggagttccct taaagatatt agctgcgcat
    18241 gaacaacggt atcggcgagt gctcattccg gaggaaccgg acgtggcgga tgatgattgg
    18301 gagactccgt tcctgatgga agtgtcaccg gtggggtcca tcaaacatgc atatctcgcg
    18361 ttaacccatc gcccccttcg aagccatctc aacgagcagc cttttgctgc aacgctatct
    18421 caataacctc tcttttgctg aacatccggt ctcctgtatg gtaatccctg tgggtgaggg
    18481 gtgtgatgtt cgaactgtat ggtataccaa gggtgtttcc ggccagcttg cttgtatggt
    18541 tcaaatgttt ctatggtatc caaccgacga gggcgcacct gttgtgttgg agttttcttt
    18601 cgtaggatga cgtgctcaag caaacattaa tggagtaatc ttcgatgatc cgaccatgtt
    18661 tcgcggttga tattgataat gtcttaggcc gcgcggagcc ggaagtgcag cggttgtttc
    18721 aggaattgac cgggaggtta tggcctgtgg gtctgtatgg gagtgctggt ggattagatg
    18781 caagtcaatt agcccctgag cttcttgagg aaattttctc ccggtttcac caggagtcta
    18841 tcccacggtt accgctgttc cctggggcaa aactggcctt gacactcatc catcgccgtt
    18901 atcggattat catcgtgacg gcccggcgtc cgtattctcg acctcagact cttcgttggt
    18961 tagaggagca tggtcttcct tttgatgcgc tgtatcacac cgaagagaaa accgacattc
    19021 ccgaatcgat aacagccgcg attgacgatc atcctcatca tattcaggcc tatcgtgctc
    19081 tcgatagaca agtttttgtc atggatcaac cctggaatcg tggaattccc catgccgatg
    19141 ttattcgtgt gaccggctgg gatgcactcc tgcagtggtt acatttccga caactccagc
    19201 aatcgccaaa aaaatttccg gagactcctt ccttccgaca tttgttctcc tctcatctca
    19261 atgtttcttc cttgacggcg gtgggtgttt cgacgggatc agcaaaacca taaaagtggg
    19321 attgttggtt atgcaggaaa ggttttttaa aaaattgccg atagaagtca gtggttattg
    19381 gttgttggga atcggcattt tccaatacaa aatctctcgt ttagtcaatt caaggaatat
    19441 tcatgatgac tctgccaatc cacacggttc tgcttgttga tgataatccc gatgactgcg
    19501 aaattgtgag agaggcatgg gaagaaatca cggttggaca agaattgcgg tgtgtgaatg
    19561 atggcaccga attgctggat tacttatacc gccgtggcct cttttccggg cgagaatgtg
    19621 cgccacgccc cagtgtgatt ctcctggatt taaatatgcc tcacatgacc ggaggggaag
    19681 ttctcattga aatcaagaag aatccatccc tggcatgtat tcccattgtg gtgttgacca
    19741 catcaattgc tccaaaggat atctgttcca ccgccggtat gggggtgaat ggatacatgc
    19801 aaaaacccaa ttcctatacc gggtatatcc agatgctcgt caatttgaga aagcactggg
    19861 cggaagtcct aaagcagcca ttgactggac gtggggggga ttgctcttcc aatgtcgcct
    19921 ggtgttgaaa gtcggtgttg tgccgtattc gccattatcc cagaccaact ttggtgtctt
    19981 ttctttcttc tctcttccat ccgtgtgggc gttcaggttt tagattggtc tttccgttcc
    20041 tctagtcatg gcctggtacg tgaagaatct tgaacggaaa gacacgcatt aactgcactt
    20101 cataagaccg ctttctcctc ttttggttgt tcgcactctt ctttcccgtc tgcccggttt
    20161 tcactacttc tgagccaaag tagagggcat gaatgtacgc tatgccgggt taagtgtttt
    20221 ctgctttctc tcccccttaa gttctctcct caataaccga cactattcta tattgactgc
    20281 cgagtagtga taacgggaga tccacctgtg tattcatcta tccagggatc ctctgattat
    20341 tcattgcgat tgaaaacctt gaggacaatg ggaaattcta tgaaaacact ttttggcttg
    20401 acgggcgcag ttgtcatgtt gggaacatca ctgttgacca caagtttggc ctttgaggca
    20461 tctgaatatt tgacattgac ccgtcaccgg gattccaatg gtcatttact ggcggggcca
    20521 actcaccacc aaccgcaacc atccgtgatt caaaaaacgg tcgttcctgc tcaaccccag
    20581 acttttgaac ggcgaagtac agagtgggga atgaatgctc aagaggtaaa actcaatgaa
    20641 ccggtgcagc cagtttggga actccaatct ccgatattgg agccttatga gcaacgtgta
    20701 gggtatcgtc gtcaaatcga agaaattgat gcgtcgttaa cctacacatt ttatgaagat
    20761 caacttgctc aggcgaatta tctctttgaa ccgaaacatg atgatgctgt ggactatgtg
    20821 caagatttcc atacggtgaa agattggata acccagtcct atggtatgcc gacgtcgttt
    20881 caagaaatct ggttagattc gttgtaccaa tatgatcaaa gtctttgggg acaagccctg
    20941 cttcgtggcc atttgaagat ggtggcggaa tggcggaacg agactacaaa tattactctc
    21001 atattagacg gtggagagga tacgataggg ttgatggctg atttcgatag cgtggcaatt
    21061 atcgctcccg ctcctatgga agccaggata attgagggaa atcctattgt ggaagacgtc
    21121 tccattgggg aaagttctgt cagggatgag cattcattgg atgctccgcc aatggaagct
    21181 ctgccagttg aaacgatgcc gaccgaatcc atatccccag aacaataccc ggacacaaag
    21241 ttataaatct ctcgtcaaat tttcagttta tttgaaatac ccattttttt tggggaggag
    21301 gccatgcctc ctcctttttt tatttataga tattgaatcg cgttggtatg gctccccgga
    21361 ttccttaggt tgtcctaagc tttaggagtt aatggtgtgc ttgaagaatc cggcctctcc
    21421 gggaaattag tagcgagggt gagtttgtgt ttaggagaga gcgcattacc aatatccaaa
    21481 tgggtaatac ggataaaacc ggccatatcc accccagtac gggtatgcat aggggtaaaa
    21541 aaatggtgaa ggataaggtc cataccggtg gtattcagaa gaatacgctg gccagatttt
    21601 gaaatgtttt atgaggagag tgggataggt atatatgtct tcatcgagac tccgttggac
    21661 agagccggat aattctcctg ccaaggtgat ccggcttccg ggagggactg tggcaggatc
    21721 taaaaatttc tgctgttccg caataaaccg tccttcggat tgagtgagtt cagttgttgg
    21781 ctcctcctca ttgatgaggg gcaattgaag gatcgtgagt tgagttctgt cttctaagcg
    21841 ttttgccgcc aggatttgcc ctccaagtat aaccagtttt cccttgaagc tgtccgggtc
    21901 atttttaatg cgcgggaaca ttaatcctgg ctcaatgtgt tctgccaggt caggaggaag
    21961 agagtttttg taggaggtag agcatccggc acaaatggct gcgagcgaaa aaaggaaaac
    22021 tagtttatag cgaaaaataa cacgtgagag tctaatggtc ctgtagtaag tcattttgct
    22081 ccttcatcat cttattacag gatattcctc cataatcaag agatgcaaat tcaacagata
    22141 cctcagatga tccggtcgcc ccgaaaggtt ttttttagac acgactggct ccacttcccc
    22201 cagatgtgat ctcaggctgg ttcctccatg gtgactatac ccgccggttc gtttactcaa
    22261 tctttgttct gtcctgcttg taaaggaggg gggggcgcca gggagttctg aggtatcgtt
    22321 aggaattctg ggatagccat gggtatgatg tcatcatgga ctgttaaagg caggtcaaaa
    22381 cgataaatat tctggaggat cgaataatgc aattatcatg agaagataag gaatatgcgg
    22441 ggtgtggcac ttttggttcg gtccttccag atggaagact ggatttggct taaataaaaa
    22501 accgaatgac tgttgtttct cattcttggt cgtgaatcgc tctcagcaat cacggcttct
    22561 ttttgggtgt ggaaaatgtg atctcggtcg caacccagtc acggttttca gggactccct
    22621 tttttgaaac ctcggccacg agtcggtccc caacctgcgg tcgggcatgt tctacatgaa
    22681 tgccattttg ttgaaacgta attttagggt gaaaggcaag ggtcagggtt tctccttttg
    22741 gggttgtgac taccacatgg tcatttcccg attccgtgac ggttcccatg acatgagcgg
    22801 catcgccgtg tgcccaggcg ggagcagtca gaaacatgga gcacagaatg gccagaatac
    22861 tgaaatattt catgatgaca actccttgga tgataaaatc aatggtggtg ctcctggggt
    22921 accacatctt ctcctgataa aaattgatca agggcttctt gttgctcccg ttctgcgcgg
    22981 gtaagagggt tatattgttt catttctgcg atttcttcag gagtgatatc ggggaaatgt
    23041 cgaataaggt gaactaattt ccaactgtcg agatcttcct ccggcggacc tttcccccag
    23101 gccggcattc cggtaaatcg aatgccgtaa tgaatgacat aaaacaattc gccatcagcc
    23161 atcgcttgaa tggctgtgtc cctgaggtca ggaacaggag gatagaaatt cggacccata
    23221 tgtgtttttc cacttccatc attggcgtgg caggtggcgc agtgatcggc aaaatgatgt
    23281 cgagcttccc ctagtgcttc agaagaatgg gggaccgggt tcgtcatctt aaggaagtgg
    23341 ctgggggtgg ctagatggcg aataaatctg gccgctttca cttcccactc gggcggagtg
    23401 gatttggcgg aaaacgcccc aggtaacaac cagagcgccg tcaatgtaga cccgaccaag
    23461 aagagaagaa caataatgaa aaatatttta attcgtttaa acataatcag ttagatgatt
    23521 ttattgtacc tgttcgggga attttttcac aagtcaaatg aacagttcac catcagagga
    23581 cgcatttttc tgtttggtga aggggatgaa attgggtagg atggtccagt gtcttgggtg
    23641 aagacattgt ggaatgaaat attcttattc cttctcaatg gaccaatcat ctttattgga
    23701 tggctatgac ttcttctccg ttgatcaaat ccctgaagaa acgggaatgt tattcacacc
    23761 ccgtgagtgg ggtcacattg gtggaaacac acatttcatg ggtgttccta accggtcagt
    23821 atgtctacaa aattaaaaaa cccgtgttgt tttccttcgt tgatttttcg acacttgaaa
    23881 aacggaggtg gttttgtgaa gaagaggttc gtctcaacag acggttagcc ccacagctgt
    23941 atctgggggt ggtccccatt actgggtccc catcaaatcc tcgcattgat ggtgatggaa
    24001 acccttttga atgggcggtc aaaatgaaac aatttcctcc cgatcaagaa tttcacaaac
    24061 ttcttgagac aggcaatctt gatgaatctg tgataagtca actcgccata gatattggaa
    24121 cattccatgg ccggatcgat tcctcaggag atacctttcc ctatggaaag ccggatatgg
    24181 tatggcgacc tatcgcagag tgcttcgagg acattccact tgcagccctc cctgagcgta
    24241 ttcaattctg tatgaaggat atcaaggaat ggactaaccg ggaatggacg cgattgagac
    24301 cattgttacg agggcgcaaa tcatcagggc agattcgaga atgtcatggt gatcttcatt
    24361 taggaaatat tgccctattt gagggccgga cctgtgtgtt tgatgccttg gaatttaagc
    24421 cggggttacg ttggattgat gttattagtg aagtcgcatt tttggtcatg gatttagaga
    24481 gcaaggatcg agtggatttg gcatattggt ttttgaactg ttatctggaa gtgacgggtg
    24541 actacgccgg gatgagtgtg ttccggttgt atgaagttta tcgggcactt gttcgagcta
    24601 aagtggcagg cttgcgtctg gctcaggtgg aatcaggaag cccggtaggg gaggatttcc
    24661 ttgaagaaat aatcagatac gtggagttgg caaatcgatt ggtaatgtct gttcctccaa
    24721 tgttgatgct gatgcatgga gtatccggga ccggcaagac gacggtctcc actgaagtgg
    24781 tgaaagcatt gggtgcagtg cgcgtgcgat ccgatgtgga acgtaaacgg atctatgccg
    24841 aacaatttaa aaaagtcgtt gatcagggag cttcagggag cttgtacgcg ccagctatga
    24901 ctcatgcaac ctatgatcgg ttatgggatc ttgcgaacgg catgttacag gcgggctatc
    24961 cggtcgttgt ggatgccaca ttccttgatg gcgcacaaag gcagtcattc ttccgactag
    25021 ctcatgaacg tcacgtgtct gttgtcatct tggacgtctg ggcgccggat gaggtgttgg
    25081 ccgagcgaat agcatgtcga gctaaacaac ggaccgatgc ctctgatgca acgattagag
    25141 ttatggaaca gcaaaggacc aaagaggatc ctttaactga acttgaagaa gacactgcta
    25201 ttcgcctgga ttcgactgat ttaccaacgg ttagatcgac gatagagagt ctaattaaac
    25261 aacgaaggaa gtatgcagat taaagccgac gccgtgaagg gggtgttgaa tccttgcaag
    25321 tctgtctaac agattggtgg ctattaaatc taaacgtaca cgctgcggaa ttctgaacag
    25381 actcctggca tcatagatta aaaaattgct cggctgaaca aggtcacact attccccagg
    25441 tttccccccc atccaaattg agcacctagt gctccgacag gggtcattgg atcatgttca
    25501 taatagactt taaccacggt aaattgttgt accgttgcac cggtaccggg aacatagacc
    25561 aaatagtcgt aaagattttg gggtaaatca caattcggaa gcgctggagg cgaaaccccg
    25621 gctccgttaa aacctccctg ctcaacgacc gtacaggttg ggttattgtt tgcgaccgtc
    25681 ccggcggtga tttgggctaa gtaaatatga tatttggatg gatgattttt aaaatccagg
    25741 gcattgtcgg tggagccgag aaggtccatc atgttctgat tggttgtcag gtttcgcgag
    25801 gctaagcttg cgccctctct cgctagctgt gtgagagcat tgcgttcgtt gatgatgctt
    25861 ccgtattcaa cgatgccaaa cgcgacgaag agaaaaatca tgagggaaaa ggcgagttcg
    25921 atagaagcgg tgccccgttc atttctcacc gttcgtaaca atgatgccat catgatccgc
    25981 ctcctaagat gaaattttca ttgcggtagg tcccctccga cgaaatgaca atggtatcgc
    26041 ctggtccaaa taacccaccg atcaattgtg tgaaaaattg atgggtaaaa ttcacccgga
    26101 ctcgcataaa ggctccggca cccccggcgc ttgccgttgc tacaactgct ggatcatcag
    26161 gatcggtcca atcggcatcg acggggaaaa tgaaaatatc tgaggagtca atgtccatga
    26221 ctgctgaggc actttgttga atcgcttgtt ttattgaatt ttcccgtgat aaggcattcc
    26281 cattgccatc gactgaagtc gcgccaacgg tcccaatacg aatgccctct cttactgcag
    26341 acgtgagggt attctggtgc acaaaaaacc aacccatttc cgctacagcg aagagagcag
    26401 tgaggaacac cagcatggtc gctcccacct ccacggccga gattcccgat tcggtctggc
    26461 atggggacat tagtttccga aaaaacctga tcaggctgtt cattggggaa ccctcctgca
    26521 tgtggatcta tcatcccagt cacgggtgtt gtgtttgccc tcgtgtggca tcgtggtggc
    26581 ctaactgata aggacaagtt taagaatatt agcaatctgt tgaaaaattc cctccagctc
    26641 tgatgagtca ggggtgtagt acgcaaaagc tgttccgctg gagagcgtcc caagaaatgc
    26701 gggatcgacg ctgcccaacc caatggtata aatttcaatg ccttgagctt tgattgctgc
    26761 ggcattgtca attgccatct gtttag
//
LOCUS       DAORAQ010000023        26671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_351_length_26671_cov_69.076984, whole genome shotgun sequence.
ACCESSION   DAORAQ010000023 DAORAQ010000000
VERSION     DAORAQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 26671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26671
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_351_length_26671_cov_69.076984"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PKM72_03860"
     CDS             <1..821
                     /locus_tag="PKM72_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNP59950.1"
                     /translation="LAELGVRNIDAYNKKVTDSQETGSPLRTMKKLSKGQSMNVEEGA
                     PLLESSQEERVLLPYIVVVIDEFADLIMVAPKDIEDRIARLAQMARASGIHLVLATQR
                     PSVDVVTGLIKANFPARIAYQVSSKIDSRTILDANGAESLLGKGDMLFLSSGTGGINR
                     LHGPYVSDEEVRGVVEWVKSQATPVYDPVALESVQEPSVDEQERDETYERARELVMTT
                     GQASASFIQRRLRVGYPRAARMIEQMEEEGLVSAPGRDGRREVLARSTAIAEIG"
     gene            818..1513
                     /locus_tag="PKM72_03865"
     CDS             818..1513
                     /locus_tag="PKM72_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNP59951.1"
                     /translation="MMPLLFLRGTLLLVIVGLMMPVSDVSAASASASVMEDVAGKVDA
                     RYAQTKDLQADFVQETILEGFTTGFTSSGRLYLKKPGLLRWDYLHPSEEQIYVDGDQV
                     MMYVPEHQQVVKGALTQMAASKGPLALLQGAGNLSQQFTILESTDGSRDADKFPSLTL
                     VPKSVGQTPPTIKKIVLKLFPDTYFIQGITLFEVSGNISRVVFDHIQANTGLSSGQLT
                     FDVPPDVVVVELP"
     gene            1575..2051
                     /locus_tag="PKM72_03870"
     CDS             1575..2051
                     /locus_tag="PKM72_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59952.1"
                     /translation="MAERILVVDDDKGVREALSEFLLSLGYTVVMAENGEEALNQYRK
                     GDFDVIMADLIMPNMDGMELLRRIRDIKNDEVIFLMITGHPSIGTAVEAINRGADDYI
                     TKPFHLEDVRLRVTKALEKQTLKGRLKTAQGLAWGLMLSIPLWLLLGIILVILFKG"
     gene            2123..2689
                     /locus_tag="PKM72_03875"
     CDS             2123..2689
                     /locus_tag="PKM72_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59953.1"
                     /translation="MSLFMQALLCVLGIVVFGASSLSAISGQITEDPTKILQKYLSLD
                     KKGVRLEAHSWQVVRPFVAWLEEPVWGQVVVISQYEVVDDVSQWEILGVLEAKIPVIF
                     EVLGAMHWERVTYVSNPHREMQYFHLKAVEDRWQIVGPQLPPHVGRQRLVDFVRWAEL
                     NESVLERKVFFNSLIQQLEHNNEKDTQK"
     gene            2686..3333
                     /locus_tag="PKM72_03880"
     CDS             2686..3333
                     /locus_tag="PKM72_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNP59954.1"
                     /translation="MTTWRAEVLFFDFDGTLIDSKIDIATSVNLTLRDLGLTIRSREE
                     IFSFVGDGVKRLLRLSVGEENCGQYEKALEIFRKHYLEHCVETTRFYPGIWEVLHHYQ
                     DRRKAIVTNKSLEYTLSIVDGLHAQDLFHHVEAPRDTMELKPEPVMLLRALEGLGIDP
                     SEAVMIGDSTNDVRAAQAAGIRSCAVGYGYGNREKVAALRPDFYCENPKDLLGLF"
     gene            3502..4122
                     /gene="thrH"
                     /locus_tag="PKM72_03885"
     CDS             3502..4122
                     /gene="thrH"
                     /locus_tag="PKM72_03885"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNP59955.1"
                     /translation="MAQPIVTCLDMEGVLFPEIWLALADKVGIQELRLTTRDVADYDV
                     LMKKRLEVLKAHRITLRDIQDVASSISPLPGVPDFIAWLRERCQIIILSDTYYEFVAP
                     LMKKLQFPTIFCHTLGVDSHGFITDYFLRQPDQKRHAVSAMKGIGFRVLAGGDSYNDV
                     SMLKEADAGIFFCPPDSIIQEFPQFPVARSYAEFQEHLGRAGGFSS"
     gene            4440..5192
                     /locus_tag="PKM72_03890"
     CDS             4440..5192
                     /locus_tag="PKM72_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59956.1"
                     /translation="MRASRIRLSVLVMVFFCFSTVTGCLGKRDWQYPPVSTGSFLNEK
                     AASPIPAKAIVLPLEDLRGNEAKEEYWKAAIPFVPYGDTKYQRPETVKHPEEVDVVKF
                     DPTNDLAQSIAAELSHAGVFSSVNFAKDEGQEPADLVFRGRLRSTDWSRRLYSYLFAP
                     VGVVFWMLGLPMSETTTALEMDLRLTPLSDPSKVLWNMTMEFEGKQLDGPYYGLENAA
                     ESYPEALQEALKPAVADLIGLAKEDPNRLMPR"
     gene            5415..5753
                     /gene="cutA"
                     /locus_tag="PKM72_03895"
     CDS             5415..5753
                     /gene="cutA"
                     /locus_tag="PKM72_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNP59957.1"
                     /translation="MEEIVVLVTVSSEAEATSLSRILVENGLAACVNIIPGVRSIFKW
                     DGKISEEQEILLLAKTGRQAFDQLVTMVKANHSYTVPEIIALPIQLGSKEYLAWIQDA
                     TKSRVGMVEG"
     gene            5830..6828
                     /locus_tag="PKM72_03900"
     CDS             5830..6828
                     /locus_tag="PKM72_03900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNP59958.1"
                     /translation="MKESEDALTVIIFRGARANPWRLKFRKSFVKYGIIVGLVLLLVQ
                     GGVITHYVYQWSQLSELGEIKQELNTSRARTSNFSTEVDGMKKRMLVLETLNRKLQTM
                     FGLEADEIQGLPTGLPGQGGEELPYEEVPYDGLQEEASLESKPAIARTGVPAVSGLDS
                     TIDRKITEIKAGLEWLNQQTELENKILEELSAAAQKKADQWASIPSIWPVKGVLTSKF
                     GPRVSPFTGKKALHAGIDIGAPTGTEVRSPASGKIVVAAYDGRMGKFVRIDHGYGIET
                     TYGHMSKINVKYGDKVQRGDLVGLVGSTGKFSTGPHLHYQVAVNDRVVDPIHYILD"
     gene            7253..8143
                     /locus_tag="PKM72_03905"
     CDS             7253..8143
                     /locus_tag="PKM72_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNP59959.1"
                     /translation="MSLDYVKFTPGFGKFMPKEYRDMVEHGPFGKKTSVSQVGTFKEI
                     LEEHPMCAGCAMTLFIRLAIIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGMADIGFAQTLHSWFRKEKFTTIMLDNEVY
                     GNTGGQESGMTNKGQVLKMAPLGKKFEKMDMLGMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGPTYIQAYTSCNIEYAIPTDKVMEDAKDVENDRYAFSEYLTDEAKQHL
                     AELYGYKEYLPKPAAAVTKG"
     gene            8206..8943
                     /locus_tag="PKM72_03910"
     CDS             8206..8943
                     /locus_tag="PKM72_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNP59960.1"
                     /translation="MAIRYNIRMAGVGGQGVVTASHIFSTAVINAGGESTIVPFYGSE
                     KRMAPVESYVRVSNEPIYEIGEITFPHILMIFHPQCITHGKSYTNPFYYGLKNEGVVL
                     INSNIPMNLEPDQAAELKDLNAKIYYLPATQMSLDVAGIDLATNMAMVGAIGAITGLS
                     TLEASGTAVKDRFLGKGFVVSGGTAALDSVVERKFKKKAELIEKNLAVVKAGWDYAVD
                     NGWTLGASSMADQTTSAAAAKAAKASV"
     gene            8974..9297
                     /locus_tag="PKM72_03915"
     CDS             8974..9297
                     /locus_tag="PKM72_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNP59961.1"
                     /translation="MYLVANIDIDICAATSCKLCTQYCPEANTIQYDTEAGKDRGLKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKHHAIKMEMIDQIGDAALTENISYLEKNTKAVLA
                     SPLKG"
     gene            9354..10115
                     /locus_tag="PKM72_03920"
     CDS             9354..10115
                     /locus_tag="PKM72_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HNP59962.1"
                     /translation="MELTKELENAIVAPGPQGFHPPSAAELGVLTPDSGMGLKFGHIV
                     EEERALEAMAHSMFTRKNATIFPGPMVLWAWNEHAADKAKAVLELAAQIPDVLIIPMP
                     DYRPKYPKVEPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCWTS
                     AICAEQGHEDAMFTVRDSDAAKIRRAAAVFKRVREEMGIKLPENSNNVRFTGLQSKVH
                     GGKTHTNPLDFSIESPVDGNAAAFGHKAEHMQKEA"
     gene            10160..11512
                     /locus_tag="PKM72_03925"
     CDS             10160..11512
                     /locus_tag="PKM72_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNP59963.1"
                     /translation="MSEALESEVKTNPQGDPITSAPEAPSIDSAKGRKDPHGDAKNQR
                     VVSPEYMFSEAPREREFITGSECAKEAVRRSNVDMSVAYPITPQSETLQLIGVLYGEG
                     YVKEFYRGEEEYGVMSAIAGASRAGVRAFTATAGPGTLRGLEPIVSWAGHRLPAVCMF
                     TCRVVNAPLAIQPDNIEIAYLLNSGMLVFHAENQQDLFDFIMKGFIISEKNDVTLPVG
                     VCCDGFFVTHARGYCAMPDRSLKLPPRDPYRGSVPVLDAENPPARLSRDAPVQKSNFM
                     AYNIHAVWQQEVWAGVERSRKYINQYMDGLLEAQDVEDADVLLVASGSAAAQSREAVR
                     QCQEKGIKAGLIKIRSLRPFPTNELRKLCGNAKLIVIPEFNYVGWLAKDVATAIYGFS
                     NAKIVAGPHVYGGMSMPVEMITDEVECGLTGKKSNTVPPSAIMGAADPKAVAEFMKSI
                     "
     gene            11611..12429
                     /gene="surE"
                     /locus_tag="PKM72_03930"
     CDS             11611..12429
                     /gene="surE"
                     /locus_tag="PKM72_03930"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNP59964.1"
                     /translation="MKPGRILVTNDDGIGSPGILALAKAMKALGQVWVVAPEKTQNAV
                     GRGMTLHKPLRVRQIRPRWYAVNGTPADCVTLAICKLLETNVPVLVVSGINKGWNLGD
                     DVTNSGTVAGALEGMLHGIPSLAVSLEDCPKASYAMAAHYAVQLAERILKSGLPEGTI
                     LNVNVPGSRMEAIAGLQFTSLSQRRYHKPVVEKIDPRGVSYFWIAGERQSWARKKPSD
                     HDAMEKNLVSVTPLHLDLTDYVALNRLKSWVKTFSPKKGTHVSSVRKLTKIKRD"
     gene            12485..13087
                     /locus_tag="PKM72_03935"
     CDS             12485..13087
                     /locus_tag="PKM72_03935"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNP59965.1"
                     /translation="MDLVKQLYDWMLSWADSPYGVPALFGLALAESSFFPLPPDVLLI
                     AMTLGSPVNAWWFATVATVGSVLGGALGYGIGWYGGQPVLKKIMGQDRMDLVHQYFQR
                     YEGWAILIAGFTPIPYKIFTIGAGAFYVDFKTFMVASIVSRGGRFFLVAGAIQFFGPW
                     IKDSIEKYFNLFSIVFIVLLILGFWIVKHQGQKLAQKTST"
     gene            13100..14584
                     /gene="cysS"
                     /locus_tag="PKM72_03940"
     CDS             13100..14584
                     /gene="cysS"
                     /locus_tag="PKM72_03940"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNP59966.1"
                     /translation="MSIVLYNTQTKKKDVFVPLIPGQISMYVCGVTVYDDCHLGHARS
                     ALTFDMIRRYFEFAGYKVKYVRNFTDIDDKILNRAKQEDVPWQEISHRYIQNFHRDMG
                     ALGIRKPSAEPRATEHIQDILHMVEGLIHKGVAYQLEGDVYFSVKNFSSYGQLSGKKL
                     EELQAGARIEVNTRKQDPMDFALWKTAKPGEPGWDSPWGKGRPGWHIECSAMSVAELG
                     PTFDIHGGGKDLIFPHHENEIAQSSAYTGKEFARFWVHNGFVTVDKEKMSKSLGNFFT
                     IREIFDKSPHSEPVTAECLRYLFLSTHYRNDLSFSDHSINEAKAALDNFYGLFQRLEE
                     SSSKPEIESAQDWETPFKDFTEKFTLAMNDDFNTPKALGAFNEIRGTINRLLGKGLSD
                     NSQKIILETLKVYGETLGLFQITVSKWNLIVGSLGVAKSEETALPINGIVGTTDKWIE
                     EKIRLRNEARAQKDFSTADTIRKELAEQGIILEDRSDGTTRWKR"
     gene            14559..15323
                     /gene="rlmB"
                     /locus_tag="PKM72_03945"
     CDS             14559..15323
                     /gene="rlmB"
                     /locus_tag="PKM72_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNP59967.1"
                     /translation="MAPHDGSDEPPATVYGFHAVLESLHSQSRPVQRIWTLRADGRFL
                     PIVKLARERGISLAVESRERLDRLAGDRHHQGVVAQVSAKEYLEGEELLDLLSIRPIP
                     ALLVVLDQIQDPHNLGAIVRSVEAAGGDGIVIPKHRAVGLTGGVAKSSAGALEHVSIA
                     RVANTGKFLETCQKRDIRTVALVPEGVQPYSEVDYTEPVALVFGSEGEGIRPIVLKKC
                     DQQVRIPMFGKMNSLNLSVAVAVTLFEAVRQRTQKR"
     gene            complement(15338..15598)
                     /locus_tag="PKM72_03950"
     CDS             complement(15338..15598)
                     /locus_tag="PKM72_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP59968.1"
                     /translation="MTNQLKPEKLDMPPIKRKRPEALTQREQEILQLIWSGLKNKEIA
                     QQLKISVKTVEAHRANMMKKVRVSNAAQLLNAAIQEGLIQIK"
     gene            15724..16497
                     /locus_tag="PKM72_03955"
     CDS             15724..16497
                     /locus_tag="PKM72_03955"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNP59969.1"
                     /translation="MSHWIVWPTVFILGTIFGSFLTVCVHRVPKGQSVVAPRSACPHC
                     GRQICWYDNVPLLSFVWLLGRCRSCHGDIPPRYPIIELANGLGYLLVVWRFGLTWPTL
                     VYAGLVSVFLVVAWIDWDHKIIPDMITLPGIVVGFLCASFLLPTGWGNSLVGILVGGG
                     FLLALAWVSPFLFGKEGIGGGDIKFLAMVGAFLGWQQAILTLMVGSVVGAVIGIVLLA
                     TKVLQKGQYIPFGPYLALGGLIAVVWGTELWHWYFHGLL"
     gene            16574..17035
                     /locus_tag="PKM72_03960"
     CDS             16574..17035
                     /locus_tag="PKM72_03960"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNP59970.1"
                     /translation="MKRSFWCTRGFTLTELIIVLGIIGVVLMLAQTWLVSQLPHWRLN
                     GATRQVTSDLLGAKMKAVVERNRQRVFFQDSHRYELLDDDNNNGKSDPGEHLVTRDIQ
                     ESYRDVKMTASNNPSFLPRGTASSLASITLSNSAGKRIITVSITGRVKVKS"
     gene            17036..17452
                     /locus_tag="PKM72_03965"
     CDS             17036..17452
                     /locus_tag="PKM72_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNP59971.1"
                     /translation="MNANIHERYPVGMCLRDQRGFSLLEVLMALVVVFMALLGFAGYS
                     VVAHTGMSASEKMTRAVTLAQEKIEDVRRGGVPISLTDPWINTEPYGSIAGAMHHQRT
                     VIIQPHTPIAGLHTVTVEVRWDHDAHSTALTTFLTQ"
     gene            17455..18075
                     /locus_tag="PKM72_03970"
     CDS             17455..18075
                     /locus_tag="PKM72_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNP59972.1"
                     /translation="MNKQIPGISKWSQQGYTLVEVVVALGLSLLTVSVVYALYIQELK
                     AQGVREHVLDMQQQARVVVDLVTREILMAGYDPRGVNHDSDLTNDFEGITYDPGKLSI
                     KADLNGNGLISDANESIVFVYDATTHLLRRNTGGGNQPFGEDIEAFVIDYLDQGGNPT
                     TNSKAIRQVGISVTARTSRPDPQYAKNGGYRTVTLHSRITVRNRQS"
     gene            18120..19391
                     /locus_tag="PKM72_03975"
     CDS             18120..19391
                     /locus_tag="PKM72_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59973.1"
                     /translation="MGGALIAALLLVAISTIMGATILFATSTDLQISGNFRRSMATFY
                     AAEAGIAETAIRLGGSSLSNSGYLGDPSPILQANWSAYVLSTSDWKSQDDQEYSGLFT
                     NYFPLNGNLSNTAVLPNSVQTTLPYWTKIRHKTEYDAERAGHSSLTPHYQDGDGSTAT
                     HSINNRGNVVFFGFGSGSGLMPRSFTSADPTPYSPVEIIISQGQVEGATSLIQVEVAH
                     PSGPPLLAPVYANSQVVFAGGTAVVEGFDTCGLLPGGRPPVWLGPAGALAGTATFTGN
                     PPMPQVGMDALDLVNTLDSLKRGAQVISGDLISVNIGAPGNPAQLYAEPLAGGFSSRL
                     AIQNLNGYGILLVRGNLNISSPFHWEGLIVVTGQVTFNGGIGTSVIQGALLADQVQIL
                     NGEVKMTLDTCSIGGSLRVLPVATLSWQQLL"
     gene            19759..21993
                     /locus_tag="PKM72_03980"
     CDS             19759..21993
                     /locus_tag="PKM72_03980"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNP59974.1"
                     /translation="MSVQPDIIYTKVDEAPELASGSFLPIIQSFAKAAGVTVGTKDIS
                     LAGRILAQFPDKLKPEQRQPDDLALLGEMVEKPDANVIKLPNISASLPQIKGAITELQ
                     SQGYAIPDYPENPKTDDEKDIKARYDKVKGSAVNPVLRQGNSDRRASASVKQYAKKNP
                     HRMGKWTKESRTHVAHMSDGDFFGNEKSATITEQAAGKGRIEFMAKDGTVKVLKDNVV
                     FDEGDVVDTTKMSVNALREFFKEQIADAKKSGILLSLHLKATMMKVSDPIMFGHAVTV
                     FFENVFKKHEQVFSELGVNPNNGLGDVYAKIGKLPAAQQEEIKADIQEALKNGPALAM
                     VDSDKGITNLHVPSDIIIDASMAAAIRTSGKMWGPDGKEYDTKAIIPDRCYAGIYQAV
                     IEFCQKHGEFNPATMGSVPNVGLMAQKAEEYGSHDKTFEAPGDGTIRIIDGAGKTILR
                     QEVEKGDIFRSCTVRDIPIQDWVKLAVKRARASSTPVVFWLNKDRAHDEQLIAKVNRY
                     LKDHDTKGLDIQIMAPVDAMKFSLKRAKDGLDTISATGNVLRDYLTDLFPILELGTSA
                     KMLSIVPLINGGGLFETGAGGSAPKHVQQFVKEGHLRWDSLGEFLALAESFAHLSQTK
                     NNKKAKILADTLDKATGKLMDNRKSPGRELGELDNAGSHVYEALYWAQELAAQDDDQD
                     LKKQFAPIAKELESKIDTILEEMKAAKGRAQDIGGYYRPDPKKVMVAMRPSATLNSIL
                     GRLA"
     gene            22107..22781
                     /locus_tag="PKM72_03985"
     CDS             22107..22781
                     /locus_tag="PKM72_03985"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP59975.1"
                     /translation="MSHVKVVTLSKIGDVPIVCKTKRDISVETPLDARDDESLGWGYW
                     DRIWPSAVALSEYLIEQFFPTKLQGARVLEIGCGTGVAGVVAAQLGAFTMFSDMVPIT
                     LEAVKDSCRRNHISNFDTCLLNWSKTIEPKEHYDLVLGSEVFYDEEILANISHVLEQM
                     LAPGGKGLFCDPNRLGWDTIERGFNEKFIVVIDEIPLNWPPRKGAGVGKMGSLYQLTR
                     RSQLAS"
     gene            23146..24342
                     /locus_tag="PKM72_03990"
     CDS             23146..24342
                     /locus_tag="PKM72_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNP59976.1"
                     /translation="MAKVLEGPGMGLLRKWGISTPNYAVVTSSDEFDQLSQVNDWLQK
                     SKLVVKAHEALGSRFKLGLVKVGLDLTAAKAAVREMLGKKMETLTIRQVIVSEAVDHK
                     EEYYAAAKSTRDGVDLLVATCGGIEVESNWDKVKHVSIETGETLTDSALSSLVKDAGF
                     SGDTAAKMAEFLKKLHACFENEDAQYVEVNPVVLSGQGELVALDAVTLLDGDAKFRHK
                     DWTFPFAAEFGRAYTKNEEEVMAVDAKVKGSVKLIEIPGGNIAMLPAGGGASVYYSDA
                     VVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIKHIIVGGAIANFTDVKKTFGGI
                     INAFRKAKAEGKLQGVKIWVRRGGPNELQGLAAMRALQDEGFDIQVFDRKTPLTDIVD
                     MAMAAK"
     gene            24378..26204
                     /locus_tag="PKM72_03995"
     CDS             24378..26204
                     /locus_tag="PKM72_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNP59977.1"
                     /translation="MSILATKDTHVVIQGGLAGVNAARRMAEFRYMIKQPLNVSAFVY
                     PPDAGKTNEIICGTELVMIPIYKTVAEATANHPEINTSLVYIGADRAYAGTMEALNDS
                     HIKTVSMITEGVPEKDAKLLGKHARKLGKTFNGPSSIGVVSAGECRLGVIGGAFDNLV
                     ACKLYRPGSFGVVTKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRIKLLAVVSGFCQESLPKGMKFGHAGA
                     KEGLKGEGSARAKSEALKKAGAIVPETFGALGPAIKATHEELLKSGQVKPIPDLAPAD
                     MPKLPKTVQESMKEGEVLVTPLIRSTISDDRGDEPLYQGYPASELINNGYDIPHIIGL
                     LWDNRLVSKQEAEIIKRIIMLSADHGPCVSGALTTIIAACAGIGLSQAVAAGMIMIGP
                     RFGGAVTDAGRWFKYAIDNKLSVDDFLVYMKKNVGPVPGIGHRVKSLKNPDKRVKELV
                     GYVKSLNMATPHLDFALEVEKITAVKKDNLILNVDGTMAAVLVDIGFPVDSLNGFFIL
                     SRTIGMIGHWTDQKKQGSRLIRLFDYLVNYASPKRREVPPLK"
     gene            26272..>26671
                     /locus_tag="PKM72_04000"
     CDS             26272..>26671
                     /locus_tag="PKM72_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNP59978.1"
                     /translation="MSLEMVKKLYTSMPGIVDKARKAFGRPLTLTEKILVSHADNFDT
                     QVWERGKAMLALRPDRVAMQDATAQMAMLQFMQAGKDKVAVPSTIHCDHLIRAEVGSE
                     KDLLRACDENKEVYNFLASAAKKYGIGFWKP"
BASE COUNT         6914 a         5484 c         7123 g         7150 t
ORIGIN      
        1 tgttagcgga attgggagtt cgaaatattg atgcctataa taaaaaagtt accgactctc
       61 aggagacggg ttctccactt cgaaccatga agaaattgag caagggccag tcgatgaatg
      121 tggaggaagg ggcacccttg ttggagtctt cgcaggaaga gcgggttctg ttgccctaca
      181 tcgtggtggt cattgatgag tttgccgatc tgattatggt ggctccgaag gatattgaag
      241 atcgcattgc ccgactcgcc caaatggccc gtgcgtcggg cattcatctc gtcttggcaa
      301 cgcaaagacc atccgttgac gtcgtaactg ggctcataaa ggctaatttc cctgcacgta
      361 ttgcttatca ggtttcttca aaaattgatt cccggaccat tttggatgcg aatggagcag
      421 aaagcctttt gggaaaaggt gatatgctgt ttttatcttc tggcaccggc ggaattaatc
      481 gactgcacgg tccctatgtc tcagatgagg aagtgcgcgg ggtagtcgaa tgggtgaaat
      541 cccaagccac ccccgtctat gacccggtgg ctttggagtc cgttcaagaa ccctcggtgg
      601 atgaacagga acgagatgag acctatgagc gggctaggga attggtgatg actacggggc
      661 aagcctcagc ttcgtttatc cagcgtcgct tgagggtagg atacccacga gcagccagaa
      721 tgattgagca aatggaggag gaaggtctgg tgagtgcccc agggcgtgat gggagacgag
      781 aggtcctcgc tcgaagtacg gccattgcgg agataggatg atgccactat tgtttctgcg
      841 cggtacgctc cttttagtca tcgttggttt aatgatgcct gtatccgatg tctcggccgc
      901 ctccgcttct gcttccgtca tggaagatgt cgctgggaaa gttgatgctc gttatgctca
      961 gaccaaagat ttgcaagctg actttgtcca ggaaaccatt cttgagggat ttacgacggg
     1021 gtttacgtct tcagggcggc tctatttaaa aaaacctgga ttgcttcgat gggattatct
     1081 tcacccgtcc gaagagcaga tttatgtgga tggtgatcag gtgatgatgt atgtccctga
     1141 gcatcagcag gtggtcaagg gagccctcac gcaaatggcc gcgtccaagg gtcctctggc
     1201 gcttcttcag ggggcaggaa acctttctca acaatttacg attttggaat ccacagatgg
     1261 gtcgagggat gcggacaagt tcccgagtct caccctggtc ccaaagtctg ttggccaaac
     1321 ccctccaact atcaagaaga ttgtactcaa actctttcct gatacgtatt tcattcaggg
     1381 tatcaccttg tttgaggtga gcgggaatat ctccagagtg gtatttgacc acattcaagc
     1441 gaatacgggt ctttcgtccg gtcagctcac cttcgatgtt ccacctgatg tcgtggtggt
     1501 cgaactaccc tgattgcaac tttcgggtct atcgtttttg atggtgttct ctgataagga
     1561 aaaggtggaa agaaatggct gagcgcatct tggttgttga tgacgacaag ggagtccggg
     1621 aggccttgtc agagttcttg ctatcgttgg gttataccgt cgtgatggcc gaaaatggtg
     1681 aagaggcctt gaatcaatat cgtaagggtg attttgacgt gattatggcg gatctgatta
     1741 tgcccaatat ggatgggatg gagttgttaa ggcgtatcag agacatcaaa aatgatgagg
     1801 tgatttttct gatgatcact gggcatccct ctattggtac ggctgtggag gctattaatc
     1861 gtggagccga tgattatatt accaaaccct tccatctcga ggacgtaagg ttgcgagtca
     1921 cgaaagcgtt ggaaaagcaa accttaaaag gtcgcttgaa aaccgcacaa ggtttggctt
     1981 ggggtttaat gttgtccatt ccgctttggc tcttgttggg gataattctg gttatcctgt
     2041 ttaaaggata agcggttttt ttctcaagtg gttctcgtgt ggtcattgct caatcgccaa
     2101 tcctttattc atggtgagcc tgttgtcctt gttcatgcag gccttactgt gtgttttggg
     2161 tattgtggtg ttcggtgcaa gctctctttc ggccattagt ggccaaatca ctgaagatcc
     2221 caccaaaatt ttacagaaat atctttccct agataaaaaa ggtgttcggt tggaggcgca
     2281 ttcctggcag gtggtgaggc cttttgtggc ttggctggaa gagccggtct gggggcaagt
     2341 ggttgtgatt tctcaatatg aggttgtaga cgatgtgtcg caatgggaaa ttcttggtgt
     2401 gctagaggcc aaaattcccg tgatcttcga ggtgttagga gcaatgcatt gggaacgtgt
     2461 cacgtatgtg tccaatccac accgtgaaat gcaatatttt catttgaagg cggttgagga
     2521 ccggtggcaa attgttgggc ctcaattgcc tccacatgtg ggccgtcaac gtctggtgga
     2581 ctttgtccgt tgggcggagt taaatgaatc tgtgctggaa agaaaagttt ttttcaattc
     2641 tttaatacaa caactagaac ataataatga gaaagatacg cagaaatgac tacttggcgt
     2701 gcggaagtgt tgttctttga ttttgacgga actttgatcg attcgaaaat tgatatagcc
     2761 acatcggtaa acctgaccct aagggatttg gggttgacca tacgatcgcg ggaagaaatc
     2821 tttagttttg tgggtgatgg ggtgaagcgg ttattgcggt tatcggtggg agaagaaaat
     2881 tgtggtcaat atgaaaaagc cctggagatt ttccggaaac attatttaga gcattgcgtg
     2941 gaaaccacac ggttttatcc tgggatatgg gaagtgctcc atcactatca ggaccgccgg
     3001 aaagccattg tgactaacaa atctttagag tatacccttt caattgttga tgggttacat
     3061 gcacaggatc tctttcatca tgtggaagct cctcgggata cgatggaatt gaagccggaa
     3121 cccgtgatgt tactgcgagc acttgagggg ttgggcattg atccatctga agcggtgatg
     3181 attggggata gtacgaatga tgtccgggca gctcaggccg ccggcatacg atcttgtgct
     3241 gtgggctatg gttatgggaa ccgagaaaaa gttgcggcat tacggcctga tttttattgt
     3301 gaaaatccga aggatcttct tggcctgttt taattaatcc cacctccatt tcattctttt
     3361 cgagggacga cagctgtcaa aagggtactg ctcgccacgg gatttttcta gttgtggcta
     3421 atgcaggaat tcttttatga tcatttgaga tttttgatgc ctttttacat tttttgaaat
     3481 tttttacgag aagaggactt gatggcgcaa ccgattgtca cctgtttgga tatggaagga
     3541 gtgttatttc ctgaaatctg gctagctttg gcggataaag tgggaataca agagctacgt
     3601 ctgaccacca gggatgttgc ggattatgat gtcttgatga aaaaacgact ggaagttctg
     3661 aaggcgcatc gcataactct tcgggatatt caggatgttg ccagttcaat ttctcccctc
     3721 cctggagttc ctgattttat tgcgtggtta cgcgaacgct gccaaattat aatattgtcg
     3781 gacacgtatt atgaatttgt agcgcccctc atgaagaaac tccaattccc tacgattttt
     3841 tgccatacat tgggagttga ttcccatggg tttatcacag attacttcct gcgccaacct
     3901 gatcaaaagc gacatgccgt tagtgcgatg aagggtatcg ggttccgggt gttggcggga
     3961 ggagattcct ataatgatgt ttcaatgtta aaggaggccg acgccggaat ttttttctgt
     4021 cctcctgatt cgataatcca ggaatttccc caattccctg tagctcgttc gtatgcagaa
     4081 ttccaggaac atcttgggcg ggcaggcggc ttctcgtcct gatatggtca tgctgatgta
     4141 ggtttgtatt cctttcccct ttttttgtta aagaggtctc tggctgatgc caagcaatat
     4201 ggaatgtttt ctgaggggga aacattgttg cctttcccca tggtacttgc tagatgtatc
     4261 acgctttata gatggctgag acgggtactg atggtgaggg gaagggaaat caaagagttc
     4321 aaattggaaa gagatgtaat catcagaaac ttgccggaaa atttttctgg atcctctgag
     4381 actgttggcc gcaaagtttt tatgccaaga gcactgacaa acttgaaagg agcatctaca
     4441 tgagggcctc tcgcattcgc ctgtctgtgt tggtaatggt gtttttctgt ttttccactg
     4501 taacaggctg tctaggaaaa cgggattggc aatacccccc ggtttctacg ggttcattct
     4561 tgaacgaaaa agctgcctcg ccaatcccgg ccaaagccat tgttcttccg ttggaagatt
     4621 tgagagggaa tgaggcaaaa gaggaatatt ggaaagctgc tattcccttt gttccctatg
     4681 gggatacgaa atatcaacgc cctgaaactg tgaagcaccc agaagaggtc gatgtcgtca
     4741 agtttgatcc gactaatgat cttgctcaat ccatcgctgc ggaattaagt catgcaggag
     4801 ttttttcctc ggtcaacttt gccaaagatg aaggacaaga gccggcagat ctggtctttc
     4861 gaggccgtct ccgttctacc gattggagcc ggcgattgta ttcgtaccta ttcgctccag
     4921 ttggagtggt tttttggatg ttaggtcttc ccatgagtga aaccactacc gctttggaga
     4981 tggatcttcg actaactccc ctaagtgacc cgtcgaaggt gttatggaat atgacgatgg
     5041 agttcgaagg gaagcagctc gatggaccat attatggtct tgagaatgcg gccgaaagtt
     5101 atcctgaggc tctacaggaa gccttaaaac cagcagttgc agatttaatt ggcctagcca
     5161 aggaggaccc caaccgtcta atgccacgct aagtgattga aattattgcc atgtgaaaaa
     5221 tcaagaaaaa atatgaaata tctggttgat atttggcaag cagaagatta ttttaatctt
     5281 caggaattgt aaaaacggcc gataatgatt aggaaatact gatatcttgc aatgaccttt
     5341 ggcagcgtct ctcatgaatt catgagagcg tttccttttc attcaagatt tcaggtattt
     5401 ggtgctattt tttaatggaa gaaatcgtcg ttttggtgac ggtaagttct gaagcggagg
     5461 ctacaagcct gtcaaggatc ttggtggaaa acgggttagc tgcctgcgtc aatattatcc
     5521 caggggtgcg gtcaattttt aaatgggatg ggaaaatttc ggaggaacag gaaattttat
     5581 tgttggcaaa aactggaaga caggcgtttg accaattagt tactatggtg aaagccaatc
     5641 atagttatac cgttccggaa atcattgctc ttccgattca gcttgggagc aaagaatatt
     5701 tagcctggat tcaagatgcc accaagagcc gagtaggcat ggtcgaaggg tagtggaagg
     5761 tgaatttggc tttattggct gtaattctaa gaaccttaga agttcttaac tttttaattt
     5821 tggtgaatta tgaaagaatc tgaagatgcc ttgacggtga tcatatttcg aggcgcacga
     5881 gcaaatcctt ggcgcctcaa attcaggaag tcttttgtga aatatggaat aattgttggt
     5941 ttagtgttac tgctcgttca gggtggggta attacgcatt atgtttacca gtggagccaa
     6001 ctcagtgaat taggagaaat aaaacaagaa ttaaatacgt cacgtgctcg aaccagcaat
     6061 ttttcaacag aagtagatgg aatgaagaag cgaatgttgg tgttggagac cctcaatcgg
     6121 aaacttcaga ccatgtttgg tctggaggcg gatgagattc aggggttgcc tactggcctt
     6181 cctgggcaag ggggagaaga gttgccatat gaggaggtgc catacgatgg gctgcaagag
     6241 gaggcatctt tggagtcaaa gcctgcaatt gcaaggaccg gggtcccagc ggtttctgga
     6301 ttagattcga ccattgaccg aaaaataacc gagattaaag ctgggctaga gtggctaaac
     6361 caacaaactg aattagaaaa taaaattttg gaagaacttt ctgctgcggc tcagaaaaaa
     6421 gctgatcaat gggcttccat tccttccatc tggccagtta agggtgtctt aacctcaaaa
     6481 tttggtcccc gcgtttctcc ttttacaggg aaaaaagcct tgcatgcagg aattgatatc
     6541 ggtgcaccaa ctggcacaga ggttcggtcc cccgcttcgg gaaaaattgt tgtagccgct
     6601 tatgatgggc ggatggggaa atttgttcgt attgatcatg gttacggcat agaaacgacc
     6661 tacggtcata tgtccaaaat taatgtgaaa tatggggata aagttcaaag aggcgacctt
     6721 gtcggtcttg tcgggagcac gggaaagttt tctacgggac cccatctgca ctatcaagtg
     6781 gctgtgaatg atagagttgt agatcccatt cattatattt tggattagcc agtcccccac
     6841 atcagctctg ggatatacgt taggcatctt ccaggtcctt ctggcatttc tttcctgcaa
     6901 cacttccatc ttcttctgat aattttggga caaaagacct aagcagtttt attcgagact
     6961 gggtcttctt ttatagatgg tgggccttct tttgtgttga attctcaaaa ttaattacat
     7021 agattagcat tgaagacgtt gaggaatttt tgtttgttgt ggggtattta ggataggctc
     7081 atgagggtct gcggaaatta attgaaaatt taagtctaac ggttaaattg acaataattt
     7141 atttcctttg ttagtttgtg tttttgctgg gtgattttac ctagcaggaa tttttgtgaa
     7201 aaataaagat ggtgatggtt atttttgttt ttaaccaagg aggaggccca atatgagtct
     7261 cgattacgta aaattcacgc caggtttcgg aaaatttatg ccaaaagaat atcgggatat
     7321 ggtcgagcat ggtccattcg gtaagaagac ctctgtgtcg caagtgggga cgttcaaaga
     7381 aatattagag gaacatccta tgtgtgcggg gtgtgcgatg acgttgttta tccgattggc
     7441 tatcattgcc ttccctaacc cggaagatac aattaccgtt gggacagcag gatgtgggcg
     7501 actggccatt tcccaggcgg ctattccttt tgtctatgga aattatggtg atcaaaatgg
     7561 agtggccagt gggttgtctc gtggactgag attgcgtttt ggtgataaac ccaaagacgt
     7621 tgtggtaatg gccggagacg ggggaatggc tgacattggt tttgcgcaga cgttacattc
     7681 ctggtttcga aaagaaaaat ttacgaccat catgttggat aacgaggttt atgggaatac
     7741 gggtggtcaa gagagcggaa tgacgaacaa gggccaagtt ttgaagatgg cgcctttggg
     7801 caagaaattc gaaaagatgg atatgttagg tatggccaag gttgcagggt gtgcctatgt
     7861 ggcgacagtg gttcccaaca acccaagaag agtagagagt gtcattaaga aagccgtttt
     7921 aattgcacga gaagtgggcc caacgtacat ccaagcctat acctcctgta acattgaata
     7981 tgcaattcca acagataaag tgatggaaga tgccaaagat gttgagaatg atcgttatgc
     8041 attttctgaa tatcttacgg acgaagccaa gcagcattta gctgaactat acggatataa
     8101 agagtatctc ccgaagccag cagctgcagt aacaaaaggg tagtctactt cctaagggaa
     8161 gaatggttta ggtgctttta aaaggtaaga gaatggaggt tgaaaatggc tattcgttat
     8221 aacattcgta tggcaggagt tggtggccag ggggtggtga ctgcatccca catttttagc
     8281 acggcggtca ttaatgctgg aggggagagc accatcgttc cgttttatgg gtctgaaaag
     8341 cgaatggctc ccgtagagag ttatgtgcgg gtctcgaatg agccaattta cgaaattgga
     8401 gaaattacct ttcctcatat tctcatgatc tttcatccgc agtgcatcac ccatggaaag
     8461 agttatacga accctttcta ttatgggtta aaaaatgagg gagttgttct tatcaatagc
     8521 aacataccta tgaatcttga accggatcaa gctgcggaat tgaaagatct aaatgccaaa
     8581 atatattacc tcccagccac acagatgtcg ctagatgtgg ccggaattga tttggccacc
     8641 aacatggcta tggttggagc aatcggggca attacgggac tttcgacttt agaggcctcg
     8701 ggtacggctg tgaaagaccg atttcttgga aaaggttttg tggtgtcagg cggaactgca
     8761 gcattggata gtgtagtgga aagaaagttc aagaaaaaag cagaattgat tgaaaaaaac
     8821 ttagcggttg tgaaggctgg ttgggattac gccgtcgaca atggatggac attgggagct
     8881 tcctcaatgg ccgaccaaac aactagtgct gcagcagcta aagcagcaaa ggcttcggtc
     8941 taaaagagaa aaagaaattg aaaggggaga gcgatgtatc ttgttgcaaa tatagatata
     9001 gatatttgtg ctgcaaccag ctgtaagttg tgcacacaat actgtccgga agccaatacg
     9061 attcagtatg atacggaagc gggaaaggac aggggtttaa agtatggatc ggcctatgtg
     9121 gccgttgatc gttgtaaggg ttgcgcacaa tgcgtctggg tttgtgacaa tatggccaag
     9181 caccatgcca ttaaaatgga aatgatcgat caaatcgggg atgctgcact cactgaaaat
     9241 atttcctact tggaaaaaaa taccaaagcg gttcttgcta gtccgttaaa gggataacct
     9301 aacaaagtct tttcattttc cagtcatttt atactcatca atcggagttc aacgtggaat
     9361 taaccaaaga actagaaaac gcaatcgtgg ctccagggcc gcaaggcttc catcctccat
     9421 cagctgcaga gcttggtgtg ctgactccag actcaggaat gggcctaaag tttgggcata
     9481 ttgttgagga agaacgggcg ttggaagcta tggcgcattc gatgttcaca aggaagaatg
     9541 ccaccatttt tcctgggcca atggtcttat gggcatggaa tgaacatgct gcggataagg
     9601 cgaaggcggt cttggagtta gccgcccaaa ttcctgacgt attgattatt ccgatgccgg
     9661 attatagacc aaagtatcct aaagttgagc cggaagaagt catcaaccct aatcatccca
     9721 atcttacgat atggggtaat aagattgagg cttgtatctt tattggtgtg cattgtcact
     9781 atgctaattt gaccctcaaa atgatccgag ctggaacaaa ttgctggact tctgctattt
     9841 gcgcggaaca aggccatgag gatgccatgt tcacagttcg agattctgat gccgccaaaa
     9901 ttaggcgagc cgcagccgtg tttaagagag tgcgggaaga aatggggatc aaattgccag
     9961 aaaatagcaa taatgttcgt tttaccggac tacaatccaa ggtccatggt ggcaaaacac
    10021 acaccaatcc actggatttc agtatagaaa gccctgttga tgggaacgcg gcggcgtttg
    10081 gccataaagc tgaacacatg caaaaagaag catgattata tttgtgaaat ttttacttaa
    10141 gtcataaggg ggtaaaacca tgagcgaagc ccttgaatca gaagttaaaa ccaatcccca
    10201 aggtgatcca attaccagtg caccggaagc accatcgatt gatagtgcaa aaggaagaaa
    10261 agaccctcac ggcgatgcaa aaaatcagcg ggtagtctct ccagagtata tgttttcgga
    10321 agctcctcgt gaacgggaat ttattaccgg tagtgaatgt gcaaaagaag cagtgagacg
    10381 ttcaaatgta gatatgtccg tggcctaccc tattaccccg caaagcgaaa cattacagtt
    10441 aattggggtt ttgtatggag aagggtatgt gaaggaattc tatcgaggcg aagaagagta
    10501 tggtgtcatg tcggcaattg ccggggcctc gcgcgcgggt gttcgagctt ttacggccac
    10561 tgcaggtcct ggaacattac gaggtcttga acccatcgtt tcgtgggctg gtcaccgtct
    10621 tcccgccgtt tgcatgttta cctgcagagt cgtcaatgct ccgctggcta tccaacccga
    10681 taatattgaa attgcctatc ttttaaatag cggaatgctc gtttttcatg ctgaaaatca
    10741 acaggacctg ttcgatttta ttatgaaggg ctttattatc agtgaaaaaa atgatgtcac
    10801 gctcccggta ggggtttgtt gtgatggatt ttttgtgact catgctcgcg gatattgtgc
    10861 gatgccggat cgtagcctta agctcccacc tcgagatcct taccgagggt cggtaccggt
    10921 cctggatgcg gagaaccctc cagctcgact gtctcgtgat gctccagttc aaaaatctaa
    10981 ttttatggcc tataatattc acgcggtgtg gcagcaggaa gtatgggctg gcgttgaacg
    11041 ttcacggaaa tatattaatc aatatatgga tggattgtta gaagcccagg acgtggaaga
    11101 cgcagatgtt ctccttgtag cttctgggag tgcagccgcc caatctcgcg aagcagttcg
    11161 acaatgtcag gaaaaaggta ttaaagctgg attgatcaaa attcgttcct tgcgaccttt
    11221 tccaacaaat gaactgagga agctgtgtgg taatgccaag cttatagtca ttcccgagtt
    11281 taattatgtc gggtggttag ctaaagatgt agctacggca atttatggat tctccaacgc
    11341 aaaaattgtg gctggcccac atgtttatgg gggaatgtcc atgccggttg aaatgattac
    11401 ggatgaagtg gaatgtggtc tgactggtaa aaagtccaat acagttcctc cttcagccat
    11461 tatgggtgcg gccgatccga aggccgttgc agaatttatg aaaagtattt aattcaattg
    11521 tccaacaatt gagaattcta aggcccgccc caaagaaatt ggggcgggcc tttctgtatc
    11581 atttcaacta ttcaggacaa tataccttct gtgaaaccgg gaagaattct cgtaaccaat
    11641 gatgatggca ttggttcgcc tggcattttg gcccttgcca aagccatgaa ggcattggga
    11701 caagtttggg ttgtcgcccc agagaaaaca cagaatgcag tagggcgggg gatgacgctg
    11761 cataaaccat tgcgggtaag gcaaattcgg ccgcggtggt atgccgtgaa tgggactccg
    11821 gcagattgtg tcactctggc catttgcaag cttttagaaa cgaacgtgcc tgttcttgtg
    11881 gtctcgggaa ttaataaggg gtggaatctg ggagatgacg tcaccaactc gggtacggtg
    11941 gcaggcgcgt tggaaggcat gctccatgga attccatccc tggcagtgtc cttagaagat
    12001 tgcccaaaag catcctatgc aatggctgcc cattatgcgg ttcaactggc ggagcggatt
    12061 ttaaaatcag gtttgccgga agggacgatt ctcaatgtga atgttcctgg ctcaagaatg
    12121 gaagcgattg ccggcctaca gttcacgagt ttgagccaac gacggtatca taagccagtg
    12181 gtggaaaaaa ttgaccccag gggagtgtcc tatttctgga tcgctgggga acgacaatcg
    12241 tgggccagaa aaaaaccctc cgaccatgat gcgatggaaa agaacctggt ttcagtcact
    12301 cctttgcatt tagatttaac cgattacgtg gcgctcaata ggctgaagag ttgggtgaaa
    12361 actttttcac ccaaaaaagg aactcatgtt tcatctgttc ggaaattgac gaaaataaaa
    12421 agggattgat aatcctcttg cccattttgg taaatattat ctattagctt ttggttccat
    12481 tgccatggac ttggtcaaac aattatatga ctggatgctg tcctgggctg attcgcccta
    12541 tggagttcct gcactctttg gcttggcttt agccgaatcc tcattcttcc cgctccctcc
    12601 tgatgtgtta ttaattgcca tgacgcttgg cagtcctgta aatgcctggt ggtttgccac
    12661 ggtggctaca gttgggtcag ttcttggagg ggctttgggt tatggtattg gttggtatgg
    12721 agggcaaccc gttttaaaaa aaatcatggg gcaagatcga atggatcttg tgcatcagta
    12781 ttttcaacgg tatgaaggct gggcaatcct tattgccgga ttcaccccaa tcccttataa
    12841 aatttttacg ataggggccg gagcctttta tgttgatttc aaaaccttta tggtggcttc
    12901 gatagtgagt cgaggcgggc gtttttttct ggtggcgggc gccattcaat tcttcggccc
    12961 atggataaaa gatagcatcg aaaaatattt caatcttttc tcaatcgtat tcatcgttct
    13021 gttgatcctg ggtttttgga ttgtgaaaca tcagggacaa aaactagcac aaaagacttc
    13081 tacctgagag ctataaataa tgtcgattgt tctctataac acccagacca agaaaaagga
    13141 tgtctttgtt ccgttgatcc cagggcagat cagcatgtac gtctgcggtg tgaccgtcta
    13201 cgatgattgc catctaggcc atgctcgaag tgcactgact ttcgacatga tccgacgcta
    13261 ttttgaattt gctggctaca aagtgaaata tgtgaggaac ttcaccgata tcgacgacaa
    13321 aatcttaaat cgtgccaaac aagaagatgt cccctggcag gaaatcagcc accggtatat
    13381 tcagaatttt caccgagaca tgggggcatt gggcattagg aagccctccg cagaacctcg
    13441 agccaccgaa catattcagg atatacttca catggttgaa ggcttgattc acaagggcgt
    13501 ggcctatcag ctagaagggg atgtctattt ttccgtcaag aatttttcct cttatggcca
    13561 actgtccgga aaaaaattgg aagaactaca ggccggcgcc aggattgagg ttaatacccg
    13621 aaaacaagat cccatggatt ttgccttatg gaaaactgca aagcctggtg aacctggatg
    13681 ggacagcccc tggggaaagg gacgtcctgg gtggcatatc gaatgctcgg cgatgtcagt
    13741 ggctgagcta ggccccacct ttgatattca tggtgggggt aaggatctca tattccctca
    13801 tcatgaaaat gaaattgccc aatcctctgc ctatacgggc aaggagtttg cgcgattttg
    13861 ggtacataac gggtttgtga ccgtggacaa agaaaaaatg tcaaaatcgc ttggcaattt
    13921 tttcactata cgcgagattt ttgacaaatc gccgcattcc gaacccgtta ccgctgaatg
    13981 cctacgttat ttgtttttat caacacatta ccgaaacgat ctgagttttt cagatcattc
    14041 catcaacgaa gcaaaagcag cattagataa tttttatgga ttatttcaac ggcttgagga
    14101 atctagttct aagcctgaaa ttgagagtgc ccaggattgg gaaactcctt tcaaagactt
    14161 tactgagaaa ttcacattgg ccatgaatga tgattttaat acaccaaagg ccctgggagc
    14221 atttaatgaa attcgaggca ctattaatag attgcttggc aagggactgt ctgataattc
    14281 gcagaaaatc atcttggaaa cccttaaagt ctatggggaa accctgggtc tatttcaaat
    14341 tacagtgagt aaatggaatt taattgtagg cagcctggga gtggctaaat ctgaggagac
    14401 cgctcttcct atcaacggca ttgttggtac aactgataaa tggatcgaag aaaaaatccg
    14461 cctacgcaat gaagcccgtg cccagaaaga tttttctacc gccgatacca tcagaaaaga
    14521 attagccgaa caaggcatca tcctggaaga ccgatccgat ggcaccacac gatggaagcg
    14581 atgaaccccc ggcaacggtg tacgggtttc atgcggttct agaatctctc cactcgcagt
    14641 ctcggcccgt ccaacgaatt tggactcttc gggctgatgg ccgatttctt cccattgtaa
    14701 aattggcaag ggaacgtggt atttccctgg ccgtcgaatc acgggagcgt ttagatcgct
    14761 tagctggcga tcgacatcat cagggcgtgg tagctcaggt ttccgccaaa gagtatctgg
    14821 agggagagga gttgctggac ctactttcta ttagaccgat tcctgcttta ttagtggtgc
    14881 tagatcaaat tcaggatcca cataatttag gcgcgatcgt ccgttcggta gaggcggccg
    14941 gtggagatgg aattgttatc ccaaagcacc gggccgtggg gctgacgggt ggggttgcca
    15001 aatcatccgc tggagcttta gagcatgtct caattgctcg ggtagccaat acggggaagt
    15061 ttctcgaaac atgtcagaag cgggacatca ggaccgtggc attggtcccg gaaggagttc
    15121 aaccctattc agaggtcgac tacacggagc ctgttgcact ggtgtttgga agtgaaggag
    15181 aaggaatccg gccgattgtc ctgaaaaaat gcgaccaaca agtgaggata ccgatgtttg
    15241 gaaaaatgaa ctcgctgaat ttgtcagttg ctgtagcagt gacactcttt gaggcagttc
    15301 gccaacggac tcagaaacga taagggccat gaagaaatta ttttatctga atcaggcctt
    15361 cctgaattgc ggcattgagc agttgggcgg cattagaaac ccgaactttc ttcatcatat
    15421 tggcccggtg ggcttcgact gttttgacgc taatttttaa ctgctgtgca atttctttat
    15481 tcttcaatcc tgaccagatg agttgcagga tttcttgctc gcgttgagtc agcgcctccg
    15541 ggcgttttcg cttaataggc ggcatgtcca atttttccgg tttcagttgg ttggtcatgg
    15601 gtacctcagg cattttctct aggcgaatta tctaagggag aatatcaggt cattttagga
    15661 ttgtaaaccg aaagcatacg taaactcacc ctagtcgaat taactacgga atatgtgact
    15721 tatatgtctc actggattgt ttggccgacc gtctttattc tgggaacgat ctttggaagt
    15781 tttttgactg tatgtgtgca cagagtacca aagggacaat cagtggtggc tcctcggtcg
    15841 gcatgtcctc attgcggaag gcaaatatgc tggtatgaca atgttcctct tttgagcttt
    15901 gtctggttgc tggggaggtg tcgttcttgc catggggata tacccccaag gtatcccatt
    15961 attgagttgg ccaatggctt gggctatctc ctggtggtgt ggcggtttgg gctcacgtgg
    16021 ccaacgcttg tgtatgcggg attagtatca gtgttccttg tggtggcctg gattgattgg
    16081 gaccacaaaa tcattccgga tatgattaca ttgccgggaa ttgtagttgg cttcttatgc
    16141 gcatctttcc tgttaccgac aggctggggc aattcgcttg taggtatttt agtggggggc
    16201 gggtttttac tagcccttgc ctgggtcagc ccttttttgt tcgggaaaga aggcattggg
    16261 ggaggcgata taaaattttt ggccatggtt ggagcttttt tgggatggca gcaggccata
    16321 ctcaccttga tggtgggatc agtggtggga gcggttatcg ggatagtgtt gctggccacg
    16381 aaggttcttc aaaaaggcca atacattcct ttcggaccct atttagcgct cgggggcctg
    16441 atcgcggtgg tatggggaac ggaattatgg cactggtatt ttcatggctt gttataagta
    16501 cccaacgaat ctgtaaggca ggtacgacca catggagtcg gttcaagtcg tcactgtaag
    16561 agggataaga gggttgaaga ggtctttttg gtgtaccaga ggttttacgc tcactgaatt
    16621 gattatcgtg cttggcatta tcggggtcgt cttgatgtta gcccaaacct ggttggtatc
    16681 ccaattacct cattggcgat tgaatggagc aaccagacag gtgacctccg accttttagg
    16741 ggcaaaaatg aaggcggtgg ttgagcggaa taggcaacga gtcttctttc aggatagtca
    16801 tcggtatgag ttattggatg acgataacaa taatggaaag tccgacccag gtgagcatct
    16861 tgtcactcgc gatattcaag aaagctatcg tgatgtcaaa atgacggcat ccaacaatcc
    16921 ctcctttctg ccaagaggaa cggcgtccag cttagcttct attacacttt ccaattccgc
    16981 cggaaagcga attattacgg tcagcattac cggacgggta aaagtgaagt cttaaatgaa
    17041 cgccaacatt catgaacgat atccggtagg gatgtgtctt cgtgatcaac gagggttctc
    17101 tttattagaa gtgctcatgg ctttggtggt ggttttcatg gcactccttg gttttgcagg
    17161 ctattccgtt gttgcccata cgggcatgtc cgcaagtgag aaaatgacca gagctgtcac
    17221 actcgcccag gaaaaaatag aagacgtgcg tcggggtgga gtgcctattt cgttaaccga
    17281 cccatggatc aatacagaac cctatggctc gattgcgggt gcgatgcatc atcaacgcac
    17341 agtcatcatt caaccgcaca ctccaatagc cgggttgcac acggtcaccg tcgaagtgcg
    17401 atgggaccat gatgcgcatt ccaccgcact gaccaccttt ctcacccagt aagaatgaat
    17461 aagcagatac caggtatttc aaaatggtcc cagcagggat atacgcttgt tgaggttgtg
    17521 gttgccctgg gactgagtct cctgacggtg agcgtggtct atgccctgta tattcaagaa
    17581 ctcaaggccc agggtgtgcg agagcatgtc ttggacatgc agcaacaggc tcgtgtggtg
    17641 gtagatttgg tgaccaggga aatcctgatg gccggctatg atccccgagg agtgaatcat
    17701 gattccgatc ttacaaatga ttttgaaggc atcacgtatg atccaggcaa gctctctatt
    17761 aaggccgatt tgaatggaaa tggcctcatc agcgatgcca atgaatcaat cgtttttgtc
    17821 tatgatgcca cgacccacct gctccggcgt aataccggtg gaggtaatca accgtttggt
    17881 gaggacattg aagcgtttgt catagactat cttgatcagg gtggaaaccc taccacgaat
    17941 tccaaagcca tacgacaagt gggaatttcg gtgacagctc gaacgtcacg gcctgatcca
    18001 cagtatgcca aaaacggtgg atatcgaaca gtaactctcc actcccgaat tacggtaaga
    18061 aatagacaat catgatcgga caaaataggc atgtaggaat aaggccttgt cttaactcca
    18121 tgggtggagc gctcattgcc gccttgttgt tggtcgctat ttccacaata atgggtgcga
    18181 cgattctgtt tgccacgtcc accgatttac aaatcagtgg aaatttccga aggtcgatgg
    18241 ccacgttcta tgccgcagag gcgggcatcg cagaaaccgc cattcgttta ggtggttcct
    18301 ctctatcaaa ttcagggtat ctcggcgatc cttctcccat cctgcaagca aattggtctg
    18361 cctatgtgtt gtccacctcc gactggaaat cacaggatga tcaggaatat tcagggctgt
    18421 tcaccaacta tttccctctg aatggaaatc tcagcaatac ggcagttctt cccaatagtg
    18481 tgcaaacgac tcttccttat tggactaaaa ttcgtcataa aaccgagtat gatgcggaac
    18541 gggcggggca cagttctctg acccctcact atcaagatgg agatggaagc accgcgaccc
    18601 attccattaa taaccgaggc aacgtggtct tctttggatt tgggtcagga agcgggctca
    18661 tgcccaggtc atttacgtca gcagatccca caccatattc tcccgtcgaa atcatcatca
    18721 gccagggtca ggtagaaggg gccacctcct tgattcaggt agaggtggcg cacccctctg
    18781 gccccccatt gctggcaccg gtatatgcga atagtcaggt cgtatttgct ggcggaaccg
    18841 ctgtcgtgga aggctttgat acctgtggcc tcctgccagg aggaaggcct ccagtgtggt
    18901 tgggccctgc aggtgcattg gcaggaactg ccactttcac aggtaatcct ccgatgccac
    18961 aagtgggcat ggatgccctg gatttggtga atactctgga tagcctcaag cgaggcgcgc
    19021 aggttatttc aggagatctt atcagtgtga atattggggc gccggggaat cctgcccagt
    19081 tgtatgccga accgcttgca gggggatttt cgagccggct tgccattcag aatctcaatg
    19141 gatatggcat cctgttggta agggggaacc tgaacatttc ctcccctttt cattgggaag
    19201 gcctcatcgt cgttactgga caagtcacgt ttaatggtgg aatcgggact tcagtcattc
    19261 agggtgccct cttggcggac caggtgcaga tactgaatgg cgaggtgaag atgactttgg
    19321 atacctgttc gatcggcggg tcccttcggg tgcttcctgt tgctaccctc agttggcaac
    19381 aactcctcta aggtctctct aagactttcc aatttctcga ctgatacatc tcaataaaat
    19441 tccttgcaag acttcctctc aataatccca ctcacttcct gtttctagtg gagttttact
    19501 tttcggttaa agtttctcgc aaacttcccc tttttccgca tcttgcatga gacttttgat
    19561 tcgtatccac atgggtcctt tgattgattg cagaagtgtg aaaccattct tacaatgcct
    19621 tacttcttgt cacggcatgg ggattggttt acaaatttca ttctctacct ggtgggtgag
    19681 atcctaaatg atgtgcttgg agcgaggtct ttgcaggaaa aaatgtgttc atagttaact
    19741 tttaacgggg taaatgccat gagtgtacaa ccagacatta tttacacgaa agttgatgaa
    19801 gcgcctgagc tggccagcgg atcgtttctt ccaatcattc agtcttttgc taaagcggca
    19861 ggtgtgactg ttggcaccaa agatatctct cttgcgggaa gaattcttgc gcaatttcct
    19921 gacaaactca agccggagca acggcaaccg gatgatttgg cgctattagg tgaaatggtt
    19981 gagaagcccg atgccaacgt cattaaattg ccgaacatca gtgcatccct cccacaaatt
    20041 aagggtgcaa ttaccgaatt gcaatctcag ggttatgcga ttcctgacta tccggaaaat
    20101 cctaaaaccg atgacgaaaa ggacattaaa gccagatatg acaaagtaaa aggaagcgcg
    20161 gtcaaccccg tattgcggca ggggaattcc gatcgtcggg cttcagcttc ggtcaagcag
    20221 tatgccaaaa agaaccctca taggatggga aagtggacta aagagtcgcg aacccatgtg
    20281 gcccacatgt ccgacggaga ttttttcggt aacgaaaaat cagcgaccat cacggagcaa
    20341 gcggctggca agggacgcat tgaattcatg gcaaaagatg gaacggtcaa ggtattgaaa
    20401 gataatgtgg ttttcgacga aggcgatgtg gtcgatacca cgaaaatgag cgtgaacgcg
    20461 ttgcgagaat tctttaagga gcaaattgca gacgccaaaa agagcggcat tctgttgtcg
    20521 ttgcacttga aggcgaccat gatgaaagtg tcggatccca tcatgttcgg ccatgccgtg
    20581 acggtctttt ttgaaaatgt gtttaagaag catgagcagg tctttagtga actgggggtg
    20641 aatccgaaca atgggcttgg ggatgtgtat gccaaaattg gcaaattgcc cgccgcccaa
    20701 caagaggaga tcaaagcgga cattcaggag gcactgaaga atggccctgc tttggcaatg
    20761 gtggattctg acaagggtat tacgaatctt catgtgccga gtgacatcat cattgatgcc
    20821 tcgatggcgg cggcgattcg gacatctggg aaaatgtggg gtccggatgg caaagagtat
    20881 gataccaagg ccataatccc agaccggtgt tatgccggca tttaccaggc ggtcattgaa
    20941 ttctgccaga agcatggtga attcaatcct gcgacgatgg ggagcgtgcc gaatgtcggt
    21001 ctgatggcac aaaaagccga ggagtacgga tctcacgaca agactttcga agcaccgggc
    21061 gatgggacca tccgtattat tgatggagcg ggcaagacca ttcttcgaca ggaagtggaa
    21121 aaaggcgata tctttcggtc atgtacggtt agggatattc ccattcaaga ttgggtgaag
    21181 ctggctgtga agcgagccag agcgtcatca actcctgtag tgttctggtt aaataaagat
    21241 cgggcacatg atgagcagct gattgcaaaa gtgaatcgct atttgaaaga tcatgataca
    21301 aaagggttgg acattcagat tatggcgcct gtggatgcca tgaaattctc actcaaacgc
    21361 gcgaaggacg gtctcgatac catttcggcc actggaaatg tgcttcggga ttatctcact
    21421 gacctgtttc cgattttgga gctaggtaca agcgccaaaa tgctgtccat cgttcccttg
    21481 atcaatggtg ggggactgtt tgagacgggg gccggaggat ccgcgccgaa gcatgtacag
    21541 caatttgtca aagaaggaca tttgcgatgg gattcactcg gagagtttct cgcgttggcc
    21601 gaatcgtttg cgcatttaag tcaaaccaaa aataacaaaa aagcaaaaat cctggcggat
    21661 acgttggaca aggccactgg aaagctcatg gacaaccgga aatcacctgg acgtgaattg
    21721 ggagagttgg ataatgcggg aagccatgtg tacgaggcct tgtattgggc gcaagaactg
    21781 gcggctcagg atgatgatca ggacttgaaa aagcaattcg ccccgatcgc aaaagagttg
    21841 gaatccaaaa tcgataccat acttgaagag atgaaagcgg caaaggggcg ggctcaggat
    21901 attggcgggt attatcgtcc tgatccgaaa aaggttatgg tcgccatgcg tcccagcgca
    21961 actcttaata gcatcttggg tcgcttagcc taggttctaa acgtaggctg gtcttccggt
    22021 attatttttt atactgggga tgtgtgcaac gagccgggtt ccgaacggag cccggctcga
    22081 tttttttggg gagaggaggt atttgtgtga gtcatgtgaa agtcgtgacc ctttccaaaa
    22141 tcggggatgt tcccattgtc tgtaagacca agcgggacat ctctgttgaa accccattag
    22201 atgcgaggga tgacgaatcc ctaggatggg ggtactggga tcgaatttgg ccttcggctg
    22261 tggccttatc agaatatctc attgagcagt ttttcccaac aaaactgcaa ggggccaggg
    22321 ttctggaaat tggttgtggg acaggggtgg ccggagtcgt ggctgctcag ctcggggcat
    22381 tcaccatgtt ttcagatatg gtgccgatta cccttgaagc ggtaaaggat tcctgccgtc
    22441 ggaatcatat cagcaatttt gacacatgtc tgttgaattg gtcgaagacg attgaaccaa
    22501 aagaacatta tgatttggtc ttaggcagtg aagtttttta tgacgaagaa attttagcga
    22561 atatttcaca tgtgctagaa caaatgcttg ccccaggggg aaaaggcctt ttttgtgatc
    22621 caaatagact ggggtgggat accatcgaac gtggattcaa cgagaagttt atcgtggtaa
    22681 tagatgaaat ccccctcaac tggcctccgc gaaaaggtgc tggggtagga aaaatggggt
    22741 ctctttacca actcactcgc agaagtcagc tggcatcctg acaatattat tttccacgtc
    22801 ctgaagctcc atgtgttcta acgaggcttt caaccggtag ttggctcaga aggcaaaata
    22861 tctagagtcg aaaattgatg ggatttgtgg tgtaaggaag gtatagcgta aatatcattc
    22921 caactatcta attttaaata ggttttccaa ttttggttgt cgtgatgttc ccatggggct
    22981 cggttatggt atgacaatgg gacttttgaa aggggagcca tgggtctttt gattgattgc
    23041 agcgggatga gggcattcgt acaatgcacc gttccgcgcg gcaggcgggt tgtgctacgt
    23101 ttgtccttaa ccatcatgat gagtccctga aggaagggtt ggattatggc aaaagttctt
    23161 gaaggccctg gaatgggtct actgcgaaaa tggggaattt caactcccaa ttatgctgtc
    23221 gtgacctcaa gtgatgaatt tgaccaatta tcccaggtca atgattggct ccaaaaaagt
    23281 aaactagtgg tcaaagccca tgaagccctt ggctctcgtt ttaaattggg attagttaag
    23341 gtcggcttgg atttaacggc ggcgaaagcg gctgtgcggg agatgcttgg caaaaaaatg
    23401 gaaacactga cgattaggca ggtgattgtt tccgaggcgg ttgatcacaa agaagaatat
    23461 tatgccgcag ccaaatcgac tcgtgatggg gtggacctct tggtggcaac ctgtggcggc
    23521 atcgaagtgg aatctaattg ggataaggtc aagcatgttt ctatagaaac gggggaaact
    23581 ctcactgatt ctgcgctttc ctccctggtg aaagatgccg ggttttccgg tgacacggcg
    23641 gccaaaatgg ctgaatttct gaaaaaatta catgcctgtt ttgaaaatga agatgcccaa
    23701 tacgtggaag tgaaccctgt tgtgcttagc ggccagggag aacttgtggc tcttgatgcg
    23761 gtgaccctct tggacggtga tgccaaattc cggcataaag actggacctt cccgtttgcg
    23821 gcggaatttg gtcgcgccta taccaaaaat gaagaagaag tcatggcggt tgatgccaaa
    23881 gtaaagggct cggtcaagct tattgagatt cccggaggca atattgcgat gttaccagcc
    23941 ggtgggggag ccagcgtcta ttattcagat gctgtggtag cccgtggtgg gaaacttgcg
    24001 aattatgcag aatattccgg cgatccaccg gactgggcag tggaagttct gactgaaaaa
    24061 gtctgctctt tgcctggtat taagcatatt attgtgggtg gagccattgc gaattttacc
    24121 gatgtcaaaa agacgttcgg aggaattatc aacgcgtttc gtaaagcaaa agcggaaggc
    24181 aagcttcagg gtgtcaaaat ttgggtgcgc cggggtgggc caaatgaact gcaaggacta
    24241 gccgccatgc gtgcccttca ggatgagggt tttgatatcc aggtattcga ccgcaaaacg
    24301 ccattaacgg acattgtcga tatggccatg gccgcaaaat aacctatttg accttgagga
    24361 tgtaggagac cgcttcgatg agtattcttg caacaaaaga tacgcatgtc gtgattcaag
    24421 gtgggctggc cggtgtgaat gccgctcgtc gtatggctga gttccggtac atgatcaaac
    24481 aaccgttaaa tgtctctgcg tttgtgtatc cccctgatgc ggggaagacc aacgaaatca
    24541 tttgcgggac cgagttagtg atgatcccca tatacaaaac cgtcgccgag gcgaccgcca
    24601 accatccgga gataaatacc agtttggtat acatcggagc ggatcgggcc tatgcgggaa
    24661 ccatggaagc gttgaatgac tcgcatatta agacggtctc catgattacc gaaggggtgc
    24721 cggaaaaaga tgccaaactt ctgggcaagc atgcccgcaa gcttggaaag acttttaatg
    24781 gcccttcgtc tattggtgtg gtgtctgcag gagaatgtcg actcggagtg atcggtggcg
    24841 cgttcgacaa tttggtggcg tgcaaactgt atcgtcctgg ttccttcggc gtggtcacca
    24901 agtccggggg attgtcaaac gagattattt ggatctgttc ccagttcgca gatggcatta
    24961 cgacggcgat cggtattggc ggtgatgcct atccgggaac cgactatgtc agctatctgg
    25021 aaatgtttga aaacgatcct cagaccaagg cggtggtgat tgttggagaa atgggcggag
    25081 atttggaaga gcgcgccgcc gaatggtacg gggctaagaa gcgaaggatt aaattacttg
    25141 cggtcgtgtc aggcttctgt caggagagtc ttccaaaggg gatgaagttc gggcatgccg
    25201 gggcaaaaga aggattgaag ggtgaagggt ctgctcgggc aaagtctgaa gcgctgaaaa
    25261 aagctggtgc catagtgcct gaaacctttg gtgctctggg accggccatc aaggctaccc
    25321 acgaagagtt gctgaaaagc ggacaggtca agcccatccc tgatctggct ccggcggaca
    25381 tgccgaagct ccccaaaact gttcaagaga gtatgaaaga gggggaagtg ttggtgaccc
    25441 ccctgattcg gtcgacaatc agtgacgatc gtggtgatga accactctat caaggctatc
    25501 cggcttctga actgatcaat aacggatatg acatccctca tattattgga ttactgtggg
    25561 ataatcgcct tgtctccaag caggaagctg aaatcataaa acgaattatc atgctgtctg
    25621 cggatcatgg tccttgtgta agtggagccc tgacaacgat tattgcggct tgtgctggaa
    25681 tcgggttatc tcaagcggtg gcggccggga tgattatgat cggtccacga tttggtggcg
    25741 cggtgacgga cgcggggcga tggttcaagt acgcgatcga caacaaactt tcggtcgatg
    25801 atttcctggt ctacatgaag aaaaatgtgg gtccggttcc gggaatcggt catcgcgtca
    25861 aaagcttgaa aaacccagat aagcgggtga aggagttggt tgggtacgta aaaagtttga
    25921 atatggccac tccgcacttg gattttgcct tggaagtcga aaaaattacc gctgtcaaaa
    25981 aagataatct cattctcaac gtggatggaa ccatggcggc ggtgttagtc gatatcggat
    26041 ttccggtgga tagcctcaat gggtttttca ttttgtcgcg aaccatcgga atgattggcc
    26101 attggacaga tcaaaagaag caagggagtc ggctcatccg gttatttgac tatctggtaa
    26161 attatgcctc tccgaaacgt cgcgaggtgc caccgttaaa gtaatgctgg tgtaagtggg
    26221 ctttcttggt cgaatcttat ccatattgaa ccgaacccag gagatggtgc tatgtcctta
    26281 gaaatggtca aaaaactcta tacttcaatg ccgggaattg tggacaaagc tcgcaaagca
    26341 tttggacgtc ctctcacgct gacagagaag atcctggtca gccatgcaga taatttcgat
    26401 acgcaagtct gggaacgcgg aaaggccatg ttggcccttc gtcccgatag agtggcgatg
    26461 caggatgcga cagcccaaat ggccatgttg cagtttatgc aagcgggtaa ggataaagtt
    26521 gctgttccga gtaccatcca ttgcgatcac ttgatccggg ctgaagtagg ttcggaaaag
    26581 gatcttttac gcgcgtgtga tgaaaacaag gaagtgtata attttctggc gtcggccgcg
    26641 aaaaaatatg ggatcggatt ctggaagccg g
//
LOCUS       DAORAQ010000024        26502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_356_length_26502_cov_68.595228, whole genome shotgun sequence.
ACCESSION   DAORAQ010000024 DAORAQ010000000
VERSION     DAORAQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 26502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26502
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_356_length_26502_cov_68.595228"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /gene="argH"
                     /locus_tag="PKM72_04005"
     CDS             complement(<1..1054)
                     /gene="argH"
                     /locus_tag="PKM72_04005"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNP59979.1"
                     /translation="MKKRAGITLPSNRSRSAGPPSPMPPQKTGDQKLWGGRFQGQTHQ
                     LVETFTASIHVDRRLYEYDIEGSIAHCKTLQRAKVLPQRECQTIIRGLQAIREDIRAG
                     RHQWKTEDEDVHMSIERRLTELIGPVGGKLHTGRSRNDQVTLDLRLYLRDTLQHLSND
                     LRLLQQSLVTLAERYMGVMMPGYTHLQRAQPVLFSHHALAYVEMVERDKGRLQDALVR
                     INVMPLGSGALAGNNYPIDRHYTASLLHFPRVTQNSLDAVSDRDYVIEVLSACTIVMM
                     HLSRLSEELILWSSQEFHFTDISDGFCTGSSMMPQKKNPDVPELIRGKTGRVYGHLFS
                     LLTTLKGLPLSYNRDLQ"
     gene            complement(1051..2055)
                     /gene="argF"
                     /locus_tag="PKM72_04010"
     CDS             complement(1051..2055)
                     /gene="argF"
                     /locus_tag="PKM72_04010"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNP59980.1"
                     /translation="MSPSSQSSRTQKSTSVAPRKKTTALPKDLLTVADIPRDTIHHLI
                     ALAQKLKAARRQGRSTFPLKGKTLGLIFEKPSTRTRVSFEAGMNQLGGQAIFLASEKI
                     QLSRGESLADTAKVLTRYLDGLVVRTFNQASLEEWAQHTSIPVINGLTDDCHPCQALA
                     DLLTIVEHRDHAKGLKLAYIGDGNNITHSLVEIGAKVGMHVVVGCPKGYEPDPRVIEA
                     AKIEGQQTGARIEITHDPKNAATDADVIYTDVWISMGQEHEQQPRMSALTPYQVNGQL
                     MKEAKSNALVMHCLPAHRGEEITEEVLDGPQSVVLDQAENRLHIQKAILLEWLGRSPS
                     "
     gene            complement(2059..3291)
                     /locus_tag="PKM72_04015"
     CDS             complement(2059..3291)
                     /locus_tag="PKM72_04015"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNP59981.1"
                     /translation="MINSPAFTRVFANMITSELQDNADRFLMNTYARLPISLVRGNGC
                     LVYDAEGREYLDCLAGIAVNILGHAHQDLVETITRQARQLIHTSNLFYTEPQTKLAQR
                     LVELSFADKVFFCNSGTEANEAAIKLARRYAHNTFGPERFEILTMINSFHGRTLASLT
                     ATGQPKLQEGFGPLVPGFRYAPFNDLEALKANITPQTAAVMLEPIQAEGGIIVPHPSY
                     LKKLREFCTEQQILLIFDEVQTGMGRTGTLFAYEQFEIQPDIMTLAKGLGGGIPIGAC
                     LATDNVAAAFEPGTHASTFGGNPLACAVALKVLELLIECGKLEQGQAAGHYLAKGLSS
                     LKEQFSCIQEVRGKGLLQGLELTIDGKPLVLECLERRVLINCTMGKVLRFVPPLIISH
                     AQIDRLLSVLSEVLSKHR"
     gene            complement(3415..4275)
                     /locus_tag="PKM72_04020"
     CDS             complement(3415..4275)
                     /locus_tag="PKM72_04020"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNP59982.1"
                     /translation="MSRTLDPSLAGTPQRDFPTMILFGSIVLTTIIGLPLYAYFYDFS
                     WVDWTMFITLYLFTGLGITVGYHRLITHRSFKCPNWIKAIFLIAGGMALENSALKWAS
                     DHIRHHARCDQKEDPYNATLGFWHSHCGWIFWKDPNRDPKYATRLLQDPLILWQDKYY
                     LPILLSGLVLPFVVGFLYNGWIGGLGCFLLAGLARTFFVLNSTFFINSICHIWGDQPH
                     GTSDSSRDSWWISLLTFGEGYHNYHHMYQSDYRNGVRWYNFDPSKWLIWTLSRLGLAY
                     DLRRQSPDQP"
     gene            complement(4716..6098)
                     /gene="ahcY"
                     /locus_tag="PKM72_04025"
     CDS             complement(4716..6098)
                     /gene="ahcY"
                     /locus_tag="PKM72_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNP59983.1"
                     /translation="MSTATMTKTASIDYKVADIRLADWGRKELCIAETEMPALMALRE
                     KYRQSQPLKGAKILGCIHMTIQTGVLIETLIELGAEVRWSSCNIFSTQDHAAAAIAAK
                     GIPVFAWKGETEEEYDWCLEQTILKGGQPWDANMVLDDGGDLTMMLHKKYPAMLERIH
                     GITEETTTGVHRLLEMLKNGTLKVPAINVNDSITKSKNDNKYGCRHSLNDAIKRATDH
                     LLSGKKALVIGYGDVGKGSAQSLRQEGMIVKISEIDPICAMQACMDGYEVVSPYKGGI
                     NTGKVEDTNSVLLQNTDLVVTATGNLNVCDSAILQSLKSGAVVCNIGHFDNEIDTAFM
                     RKNWEWEEVKPQVHTVYRDKASKDHLILLSEGRLVNLGNGTGHPSRIMDGSFANQVLA
                     QIYLYERKFADQKGGTITVQVLPKHLDEEVAAHMVRGFGGVITKLTQTQAAYIHVPVN
                     GPFKEDSYKY"
     gene            complement(6199..7350)
                     /gene="metK"
                     /locus_tag="PKM72_04030"
     CDS             complement(6199..7350)
                     /gene="metK"
                     /locus_tag="PKM72_04030"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNP59984.1"
                     /translation="MRQTNFLFTSESVTEGHPDKIADQISDGILDAIIAKDKNCRVAC
                     ETILTTGIAFVAGEISTRAYVEIPEIIRETIREVGYADATWGFDYSTCSVLTAIHNQS
                     GDIAMGVDSGGAGDQGLMFGYASNETPELMPMPIILAHRLTRRLAEVRKKNILPWVRP
                     DGKSQVTIEYKNGKPIRIDTIVVSTQHSDAVTSKKIEKDVMEKVIIPVMPKKLFNPKS
                     TKFHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGVGSHGGGAFSGKDPSKVDRSASYM
                     ARYIAKNIIAAQLAEKCEVQLAYAIGVAEPVSVLVDTKGTEKAPIDRIEKLIIKHFPL
                     TPRGIIEHLKLRRPIYKRTAAYGHFGRNEPGFTWEKTDKASLLRKGAGL"
     gene            8122..10221
                     /locus_tag="PKM72_04035"
     CDS             8122..10221
                     /locus_tag="PKM72_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP59985.1"
                     /translation="MYKTIYVPVDNSDHSNMAVELGVQFAKIFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDFVDQKCAEANIPL
                     ERRSLEGRNWKAIAEDISKNGYDLTIMGALGVGAVKDSVIGSNTERVIRRIRNSDMFV
                     VKDTKPMNGGKIVVAVDGSHYSFGGLKTALALGKALNKPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEVIDSGLAKIYQSHLDVSRDVAQDEGADIKTTLLDG
                     KAFEKIIQYVRKEQPWLLIVGRIGVHSDDDMDIGSNAENLLRSAPCNVLISNKKFVPP
                     IDTQAEYTIAWTEEALRRMEKIPVFARGVAKTAIHRYAIEKGHTIISNSVIDDAVGDI
                     LPKGAMDAMKTLGGTLDAAGVDRNAMQAGDEVAQDLMGSTLSGMMSQVVEEKPSEASA
                     PMSAGNKSYLARMSRDYYVCGGCGYIGKGDQPVMCPVCGLDGAQFKQVDKSIFDAAAK
                     AEGGLETQVSYDDIPMQWTKDAREAIRAVPAGFQRRRAKAKIEKTARKLGMTTITLEY
                     SGAMIQEAASEDYTPIFANKTAGETAPMVSESKTENTGNNNGADPYVWEPDALQRLER
                     APAGFMRDCTKALIIKHAEKIGTTTITLDVANQGIEQAKTTMEEAMKTGNVKDIVAKL
                     TGTGAPSEAQSGAGHNG"
     gene            10214..11596
                     /locus_tag="PKM72_04040"
     CDS             10214..11596
                     /locus_tag="PKM72_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP59986.1"
                     /translation="MGKSLPVLNSFLNTLNSLGAGAISKFESFRPDSGAPGDGRTVDD
                     FKPYLVALNLTKRCNLKCDHCYLDATTKMGGGQDELTTEECFQLIDQIAQVNAGSLLV
                     ITGGEPLVRPDILEIARHAVEKRFMVVFGTNGMLIDDKMAKAMVDIGVMGVGISIDSL
                     DSATHNAFRGLPGAWEGAMAGIEACKRNGLQFQVHFSAQPMNYKELPAVIDWSHDLGA
                     KVLNVFFMVCTGRGEELTDITPSQYEEVLTYMVESQDKYQDMLVRARCAPHFKRLAYE
                     KDPNSPITKATGYMGGGCLAGTNYARVTPNGDLTPCPYMPLSAGNIRDTSFVDLWENS
                     EVFNSFRYPHLKGKCGDCEYSEICGGCRARPYVDHGDAMDEDEWCLYTPKGGEKIKVA
                     FNMPEASSVEWEADAEERLSRIPYFLRAMVKKGVERHAVEQGLPIVTIELMEELRKRR
                     FGNEKPVFKF"
     gene            11596..12123
                     /locus_tag="PKM72_04045"
     CDS             11596..12123
                     /locus_tag="PKM72_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59987.1"
                     /translation="MESLLGVLTDLNQIIPILVHWLHLLSAVVWIGGLAFLVMAVTPC
                     LKNTVPKQFIRPISETFYQQYKRVVGILLVVILFTGGFNLHYVSEGMVMQSGEGVSHH
                     AKYLTVFFIKLFLVLGILTIFLYTIIFKNEPTGGETSEELEEMAIEPVPFQRVALWMG
                     VFIILCAAAMKHLHV"
     gene            12540..13955
                     /gene="der"
                     /locus_tag="PKM72_04050"
     CDS             12540..13955
                     /gene="der"
                     /locus_tag="PKM72_04050"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNP59988.1"
                     /translation="MARSSRSRTPRSRTRPSLESSLPSDLPVEVPLEPPPHRPLVALV
                     GRPNVGKSTLFNRIVGHRTAIVDDVSGVTRDRNTAECDYQNRIFTLVDTGGLDLTTDD
                     VIVEQIKFQTQAAIEEADLIIAVMDGRVGLSPLDADLVKLLRPVTKPVFLAVNKIDTP
                     QAEPLLADFYKLGAKEIFPISAEGGLGVDELLEALLPYLPQATENEVQREIPRIAVVG
                     RPNVGKSTLVNAILGEQRLIVSDIPGTTRDPIDSMVSLHGRRYIFTDTAGLRRRGRIE
                     RGIEGYSVVRAIRALGRSDVAVLVLDGVEGVTEQDTKIAGLISKQGRGCIILVNKWDL
                     RADDREAYPAYNLELARRFPFFTYVPVVFGAAIQSETLTRIFPKIDQVVADFSKRIQT
                     RKLNLFVQELLEKNPMTLVRGNPKKSVFITQVATKPPTFALFVKSAPKVPASYLRYLE
                     NSIRTEYGFEGIPLRILLRSQ"
     gene            14063..14449
                     /locus_tag="PKM72_04055"
     CDS             14063..14449
                     /locus_tag="PKM72_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP59989.1"
                     /translation="MNRLLWICVLILLGFTASYETRDVEAQPEGKQGRDLYRIHCLEC
                     HGAEGRGDGPRAALLAPRPGNLVSAATSTKTDEELLEILANGVPRTAMHGWSEQLSEE
                     QRRNVLAYVRTLVHFHDLSSPPSPGP"
     gene            complement(14446..15849)
                     /locus_tag="PKM72_04060"
     CDS             complement(14446..15849)
                     /locus_tag="PKM72_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNP59990.1"
                     /translation="MSDYQSQLHTYIQDQRPNFEDLLGQMVEVPSVSSDPEHAHDMHR
                     MAQLAVQYLEQFGAKASIVNTKGYPSVAGEWVAGRQHPSLTIYNHLDVQPAQEPEWRQ
                     SPFAFKKDGDRYCGRGATDDKGPALTALFAAKFAREVGIPLNIRILWELEEEIASPNF
                     PEVLQNRKAVPPSDSVLVSDTIWVAKDRPALSCGLRGLLVAKLLLRTGETDIHSGLTG
                     GGARNPLAELCDAAYSCLDAKTGRVKIPGFYDDVVPPTRTEMKNFLASGFQVRLFKEA
                     YQLRSLRTSDPQDLLKRIWASPTFEVHGLTGGHMGPGVKTIVPAWGELKVSMRLVPNQ
                     HPSKIFRLLKKHLRARNPDIEVINDGELEPYRGISDGPYAEALQEAVAQGFGRRPVFV
                     REGGSIGAIAVLQRAWKVPILFMGLSLPEHGYHAPNEYFDWGQASGGIQAFVHYFDHL
                     SRMRPDKSKTRLKPTTG"
     gene            complement(15957..17087)
                     /locus_tag="PKM72_04065"
     CDS             complement(15957..17087)
                     /locus_tag="PKM72_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNP59991.1"
                     /translation="MDHESPTHSTPSENNPTPPPSLLNPIAPTDSLPSTNPISPPPLI
                     MPISKDAMLRERTLETSSRGIGHWHSIQPDRLDSRDEVDEFPPPTPSAFSQWGLPILL
                     FGLTIFTTLWAGAFQVNMKPVNGAWDFLMRYPGSLVNGIPFAATLLGILVTHEFGHYL
                     LSRIHRVPASLPLFIPGPPHFIGTFGAVIRMRSAIMNRRALFDIGVAGPIAGFIAAVV
                     AIIIGLSFSHIVPRSQTYGLQLGEPLLLQWFAWLMFGPIPPTHDIVLHPIAFAAWFGF
                     FVTAINLLPLGQLDGGHVAFAVLGRRQRSLALGTIPILIYLGLTGWPGWILWVGLASL
                     VGIAHPPVMDPHTVLGKHRRWVAWAALAIFIVTFAPVPFSVG"
     gene            complement(17138..17989)
                     /locus_tag="PKM72_04070"
     CDS             complement(17138..17989)
                     /locus_tag="PKM72_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP59992.1"
                     /translation="MSLEDEFCDIIKKARFGQGLGIETLAELAKMEQADMEDLEKGNR
                     TPTKSEIHGISQALHLRVGPLINLTLDGWLPQPQPEWTTEHDLVQTIKGDIGGYEVKG
                     YLLTDPVTNQTIMIDTGYNAKQMLSSISQRELTLIGVCLTHGHTDHAGGLKEILAQWP
                     VPVYLGNGDFDLLPWTPPDASIKIPADHQMIALGDLTVECLATPGHTPGGFCYLLSLK
                     GQTLCFVGDTLFSGSVGRSNPFSLYPRHLQSVRHTLLHLPKDTALFPGHGPSTTVLEE
                     QAHNPFA"
     gene            18383..18700
                     /locus_tag="PKM72_04075"
     CDS             18383..18700
                     /locus_tag="PKM72_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNP59993.1"
                     /translation="MKNLLIAGFLGFCLLNLTGCMGVASPVAGWAYTEAKFGNQVTDG
                     AAGTKTGTACATSILAMVATGDASQEKAMANGGITQITYVDHSAKNILGIWGEWCTIV
                     HGS"
     gene            19530..20096
                     /locus_tag="PKM72_04080"
     CDS             19530..20096
                     /locus_tag="PKM72_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNP59994.1"
                     /translation="MDDIEIYLAGYGMGSQPLNKGVSFKSREVSGEKLNGDPGLGFKV
                     GFFPHFASGFLGLELESFGNNNSIRFPIDSSLRPTKGNSSLITYQSMVNLIVRYPGTL
                     FRPYAGIGGGLSNGILRNADIPGRKDKNFETGTAPGYQFLGGMQLFITERWFVFGEYK
                     YFSANYHWKELSLNFRSEYFLGGIGFLF"
     gene            complement(20160..21974)
                     /locus_tag="PKM72_04085"
     CDS             complement(20160..21974)
                     /locus_tag="PKM72_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNP59995.1"
                     /translation="MNEQAVRDKLHEQRYWHLLLHYQPSLFGGHTSEVDDPGFFLSEN
                     GKEDPEAELRATISFFFSDKLVGRSQQPAQCAFVARYHWLKEKLKFEEAKLPSHECER
                     FHHWYDEFDAEFISMIFPSAFMGNPASMFGHTFLRVDPKGQTEQTRILAYTINYAAEV
                     PPNPGIEYAYKGVFGGYKGFFSTIPYYMKVKEYGDIENRDIWEYRLNFSPDQIHRLLM
                     HVWELGNAYFDYYFFKENCAYHILSLLEVADPNLHLTDQFHFATIPGNTIRLLFRYKG
                     LVKESVYRPARSTLLKRKRSDLEKQETSLLPKLLNDPHLTERFEFRQLPRNRQIFLLD
                     FASDVLRYKAATDQDKAEDYKAKNRSILVTRSQIKIPSPSFHVEPFSLSPDHGHNSLR
                     VGEGFGWRNHEIFEEISVRAAYHDLLDPDAGYTQDAQIELLDLRLRHYHSRNQFRVER
                     FTFANILSLAPIDSLFISPSWKFNVGMDTVKTSSCDLCSNGNINAGIGGAQETQIFKR
                     EVFFLLGEIDANVSGAYKENHRVGGGISGGVMATITPRWKWLISAGYLYYPLGDRSHD
                     IPVSVGQRWTFAKNFAVRATFTHHDRDNEVLTVLHGYF"
     gene            complement(22132..22644)
                     /locus_tag="PKM72_04090"
     CDS             complement(22132..22644)
                     /locus_tag="PKM72_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNP59996.1"
                     /translation="MAKKPLALVSTFFLVMGMSGMALGNPPNNGPGCGLGKLAWEQSS
                     QNESIGGQVLMATTNGTGFQTFAISSGTSGCTNDGKIFASEKVNVFTALNFDNLSQEM
                     AQGQGEHLTSLATLMGIPQEQHPEFFSMTQEKYSTLVQAGESSPKAVVKAINNAMQGH
                     IILAQTTTNK"
     gene            complement(22806..23900)
                     /locus_tag="PKM72_04095"
     CDS             complement(22806..23900)
                     /locus_tag="PKM72_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP59997.1"
                     /translation="MNIAEKMKHDWNERAQHHARFWIATEAYHTEEMFTQSGKNTAQA
                     LLATLPVPHLPSWNVLDIGCGIGRVLKALASHFHHLVGVDVSSAMIAQSKQWLADFPH
                     IHTYETSGVDLLEFPHSSFDLVYSYVTFQHMPRPVFERYLREIHRVLTPKGYLVFQLP
                     IGHFQDVPLEDTIGIRSYSNQEIEESLRRNGLGFHPSSDPEFTELSDPHSHRFRLAQK
                     IGPISPPVPMDWEELKQPHFVSEWDNHLYGIYADHCADAGNYQEGIQTLQMLVNKNPV
                     NLEGRLRLAALLIETGQLPKAFATMKEIITLHPGYEEGHRTLRQLIEKCPDLKPARIP
                     SLSTTSHHSPADASRNIPALTNREYSTTEV"
     gene            complement(25406..25660)
                     /locus_tag="PKM72_04100"
     CDS             complement(25406..25660)
                     /locus_tag="PKM72_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP59998.1"
                     /translation="MLKIFGGMIIGSLMTMILLGGVSAADKVLANAQTLYFEQIQRPD
                     TTTTLLLLVFSSIFLAVLTLWPAKPVLDTKKITKQLRRHC"
     gene            complement(25938..26306)
                     /locus_tag="PKM72_04105"
     CDS             complement(25938..26306)
                     /locus_tag="PKM72_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP59999.1"
                     /translation="MKKVLLVDDDLNAREALRLVLESQQLECIEASNGSEAIAWLSTH
                     RADLIISDNQMPVLAGLDFIDQIKLKEKEPIQTPVILLSGHLKEQDKHRARQAGVFAI
                     LDKPCNFSQFLSMVQLALDQ"
BASE COUNT         6785 a         5776 c         6720 g         7221 t
ORIGIN      
        1 cctgaaggtc tcgattgtaa ctgagtggca atccttttaa ggtggtcagg aggctgaaca
       61 aatgtccata tacccgacca gtttttccac ggatcagttc cgggacatcc ggattttttt
      121 tctgtggcat catactgctg cccgtacaaa atccgtcgga aatatccgtg aagtggaatt
      181 cttgcgagga ccataaaatg agttcttcac ttagccgaga gaggtgcatc atcacgatgg
      241 tgcaagcgct gagcacttca atgacgtaat ctcggtccga cacggcatcc aggctattct
      301 gggtcacgcg agggaaatgt agtaaggaag ccgtatagtg acggtcgatc gggtaattgt
      361 ttccagctaa tgcgccggac cctagaggca tgacgttaat ccgtaccaag gcgtcctgaa
      421 ggcgcccctt gtcccgctct accatctcaa cataggccag ggcatgatgc gaaaagagaa
      481 cgggctgagc ccgctgtaga tgtgtatagc ccggcatcat cactcccata taccgctcag
      541 cgagggttac gagggactgt tgaagtaacc ggagatcatt gcttaggtgt tggagagtat
      601 cccgaaggta gagtcgaagg tccagggtga cctgatcatt ccgactccgg ccggtgtgca
      661 gttttccccc aacaggtcca atgagttcag tcagccgccg ttcaatgctc atatgcacat
      721 cttcgtcctc ggtcttccat tgatgccggc cggcgcgaat gtcctcgcga atggcctgga
      781 gccctcgaat gatggtttgg cattctcgct gcggcaagac cttagcccgt tgtaacgttt
      841 tgcaatgggc gatactgcct tcaatatcgt attcatacag gcggcggtca acgtgaatcg
      901 aggccgtgaa ggtttccacg agttgatgtg tttggccttg aaatcgccct ccccagagtt
      961 tttgatctcc tgttttttgt ggcggcattg gagaaggagg ccctgcagaa cgggatcggt
     1021 tgctgggcag ggttatgccg gcgcgtttct tcatgaaggc gaccttccta accattcaag
     1081 caaaatagct ttttggatgt gtaggcggtt ttcggcttgg tcgagaacta ctgattgtgg
     1141 gccgtccagc acctcttcgg ttatttcctc cccccgatgg gctggcaggc aatgcatgac
     1201 cagcgcattg gattttgcct ctttcatcag ctgaccgttg acctgatagg gggtcaaagc
     1261 gctcatgcgt ggttgttgtt cgtgttcctg gcccatgctg atccacacat ctgtgtaaat
     1321 cacatcagcg tccgttgctg cgtttttagg atcgtgggtg atttcaatcc tggcaccggt
     1381 ttgttggcct tctattttgg ctgcctcaat gacgcgggga tcaggttcat agccttttgg
     1441 gcatcctacg accacgtgca tacctacctt agctccaatc tcaacaaggg agtgcgtgat
     1501 attgtttccg tctccaatat atgccagctt taagcctttc gcgtggtctc tgtgttcaac
     1561 aatggtcaaa agatcagcca aagcctggca ggggtgacaa tcgtccgtta acccgttgat
     1621 aacaggaatc gaggtgtgtt gagcccattc ttctaaggag gcttggttga atgttcttac
     1681 aacgagtcca tccaaatatc gtgttaagac ttttgccgta tcggctaagc tttccccacg
     1741 actcaattgg attttttcgg acgcaagaaa aatggcttgt ccacccagtt gattcatgcc
     1801 tgcttcaaac gaaaccctgg tccgcgtaga aggtttttca aaaataagac caagcgtttt
     1861 gcctttcaac ggaaaggtag agcgcccctg tcttcgggca gccttcagtt tttgagcaag
     1921 tgcaatcaaa tggtgaatcg tatcccgggg aatgtcggcc accgttagca ggtctttggg
     1981 gagagctgtc gtctttttgc gtggtgcgac agaagtagat ttttgagtac gagaggactg
     2041 acttgatggt gacatgattt atcgatgttt agaaagaact tcagagagga cggacaagag
     2101 ccggtcaatc tgtgcgtggc tgatgattaa gggaggcaca aatctgagaa cttttcccat
     2161 tgtgcagttt atgaggaccc gtcgctccaa gcattccaag accagaggtt ttccgtctat
     2221 ggttaattct agaccctgga gtaggccctt tccacggact tcctgaatac aggagaattg
     2281 ttctttcagg gaactgagtc ctttggctaa atagtgaccg gcagcctggc cctgttcaag
     2341 tttcccgcat tcaataagga gttctaagac ttttaaggct accgcgcagg ctaaagggtt
     2401 gcctccaaac gtggaggcat gcgtgcctgg ctcaaatgcc gctgcgacgt tgtcggtggc
     2461 caaacaggcc ccaatgggaa tgcctccccc tagcccctta gctaacgtca taatgtcagg
     2521 ttgaatttcg aactgttcgt aagcgaataa cgtccctgtt cgtcccatcc ccgtttggac
     2581 ttcatcaaaa atgaggagaa tttgttgctc ggtgcagaac tctcgaagtt ttttcaaata
     2641 ggaaggatga ggaactataa tcccaccttc agcctgaatg ggttctaaca tcacagctgc
     2701 cgtttgtgga gttatgttgg ctttcaacgc ctctagatca ttgaatgggg cgtagcgaaa
     2761 tccaggaaca agcggtccga agccttcttg taattttggc tgtccggttg ccgtgaggct
     2821 cgccaatgtt cgtccatgaa aagagttgat catggtcaga atttcaaagc gttccggacc
     2881 gaaggtgttg tgggcatacc gtctggctaa cttaatggcg gcctcgttgg cttctgttcc
     2941 gctattgcaa aaaaagactt tgtcggcaaa ggacagttct accagccttt gggccaattt
     3001 ggtttggggt tcggtgtaaa acaggttgga ggtatgaatg agttgtcggg cttgtcgtgt
     3061 gatggtctct accagatcct ggtgggcatg acctaaaata ttgacggcaa tccccgcgag
     3121 acaatcgaga tattccctgc cttctgcatc atacacgagg catccatttc cccgaacaag
     3181 tgaaatgggt aggcgggcat aggtattcat cagaaatcta tccgcattat cttggagttc
     3241 acttgtaatc atattggcaa aaacccgcgt gaatgcaggc gaatttatca cacgacttct
     3301 tcggttcgca ataatgagct aggtgggaga ttggtcggat aacaaaaagg caggagaaga
     3361 gactccttcc cagagcccaa ttcaggtaaa ggagccttta aatgctaaat atcgttacgg
     3421 ctggtcagga gactgccgcc gaagatcata ggctaagccc aatctgctca atgtccatat
     3481 aagccacttt gagggatcaa aattatacca gcgaacccca tttcgatagt cactttgata
     3541 catatgatga tagttgtgat atccctctcc aaatgtgagt aatgatatcc accaactatc
     3601 cctgctcgaa tcagatgtgc catggggttg atcgccccag atatggcaaa tggaattgat
     3661 gaaaaacgtt gagtttagaa caaagaaagt acgtgcaagc ccagccagca gaaaacatcc
     3721 gagaccgcca atccacccat tgtacagaaa tcccactacg aatggcagca ccagtccaga
     3781 caaaagaatg ggaaggtaat atttgtcctg ccacaaaatc agagggtcct gaagaaggcg
     3841 tgtggcatat ttggggtcac gattgggatc cttccagaaa atccagccac aatggctgtg
     3901 ccaaaagccc agtgtggcgt tataggggtc ttccttctgg tcgcaccggg catggtgcct
     3961 aatatggtcg ctggcccatt ttaacgccga gttttctaac gccataccgc ctgcgattaa
     4021 gaatatggct ttgatccagt ttgggcactt gaagctccta tgggtaatta accgatgata
     4081 gcccactgta atgcctaatc ctgtaaatag gtaaagcgtg ataaacatgg tccagtctac
     4141 ccaggaaaaa tcgtaaaaat aggcatacaa agggagcccg atgattgtgg tcaacacaat
     4201 tgatccaaac agaatcattg tggggaaatc tcgttgtgga gttccggcta aggatggatc
     4261 taacgttcgt gacatagctg actaagtcct cttatatctc cagtcaccaa agagaatgtt
     4321 ccccatacaa gagggaaaat tcaacggcga gagatttgtt tgtgaaataa aacctaggat
     4381 tgaaatctct tttacatata cgaaagtggg ttgttgaaac cgactaacac gggccttgat
     4441 tttcccgtgt aaaagagtgg cttgagatta taatgtaaca atttagccgg gaagaggcaa
     4501 tcccatggca gaattcaata atgacactct ttatgtgtcc agaataccag atgtaatgaa
     4561 aaaagctagg ggaccctgta caagccaagg ccccctaaat actactgtct ggtatttatt
     4621 tttgaattcg caaacaaaat tgcggaccag tgcatttgtt atggtctttg ttaagaagtc
     4681 ccttgaagaa atatcagaac tgcctgagct atattttaat acttgtagct gtcttctttg
     4741 aatggtccat taactgggac gtgaatatag gcagcctgag tttgggtgag tttagtgata
     4801 acccctccaa agccacgaac catatgggca gccacttctt cgtcgagatg tttcggtaaa
     4861 acttgcactg taatggttcc acctttttgg tctgcgaatt tccgttcgta gaggtagatt
     4921 tgcgcgagga cttggttggc aaatgaacca tccattattc tggaagggtg tccggtccca
     4981 tttcccaaat tcaccaggcg accttcggac agtaaaatga ggtgatcctt gctggctttg
     5041 tcacgataca ctgtatgcac ctgaggtttt acttcttccc attcccagtt cttccgcata
     5101 aaagcagtgt cgatttcatt gtcaaagtgt ccgatattgc aaacaaccgc cccagatttc
     5161 aatgattgca gtatggctga atcgcagaca ttcaagttgc cggtagccgt gacgaccaaa
     5221 tctgtgtttt gaagaaggac ggagttggtg tcttcgacct tgcctgtatt gatgccacct
     5281 ttgtagggag agaccacttc atagccatcc atgcaggctt gcatggcaca gattggatca
     5341 atttctgaaa ttttgacaat catgccttcc tgtcggagtg attgagcgga gcctttcccg
     5401 acatcgccgt acccaatgac cagggctttt ttccctgaca ggaggtggtc agtggcgcgt
     5461 ttgattgcgt catttaaact gtggcgacag ccatatttat tgtcattctt tgatttggtg
     5521 attgaatcat tcacattaat ggctggaacc tttagtgttc cattttttaa catttccaat
     5581 aaacgatgga ccccagtggt tgtctcttca gtgattccat gaattcgctc cagcatagct
     5641 ggatattttt tgtggagcat catggtcaag tctccaccgt cgtccaggac catgtttgca
     5701 tcccagggtt gtccgccctt taaaatggtt tgctccaagc accaatcata ttcttcttct
     5761 gtttcaccct tccaggcgaa tacaggaatt ccttttgcgg cgatggcagc ggcagcatga
     5821 tcttgtgttg aaaaaatatt gcaggatgac cagcggactt cagcacccag ttcgatgagc
     5881 gtttcaatta acactcctgt ctgaatggtc atgtgaatgc agccaagaat ttttgcgcct
     5941 tttaatggct gggactggcg gtatttctcc cgaagagcca tcaatgccgg catttctgtt
     6001 tcggcgatgc ataattcttt tctcccccaa tcagccaaac ggatatccgc aactttgtag
     6061 tcaatcgagg cggttttggt catagttgca gtgctcatag gaattgtctc tccttctctg
     6121 tttgagtatt gaaggtctgt tgaaaagggg gaaggtttgg tccccttccc catcattcgg
     6181 cgggctaggc acttgaaatt ataagcctgc gccttttcgg agcaagctgg ctttgtctgt
     6241 cttctcccaa gtgaatcctg gttcgtttct gccaaaatgt ccgtatgcag cggtcctttt
     6301 atatatagga cgccgcaact tgaggtgttc aatgataccc ctgggggtca atggaaaatg
     6361 cttgatgatg agcttttcaa tgcgatcaat aggtgctttt tcggtgccct ttgtatcgac
     6421 caaaacggaa accggttcgg cgaccccaat ggcatatgcc aattgtactt cacatttttc
     6481 tgccaattgg gccgcaatga tatttttcgc aatataacgg gccatatatg aggctgatcg
     6541 atctacttta ctggggtctt tccctgaaaa agcgcctcct ccgtgactgc caactccgcc
     6601 atatgtgtct acgatgattt ttcttcctgt aaggccagta tcacccatgg ggccaccgac
     6661 gacaaatcgc ccggtggggt taatatggaa tttggtggat tttggattga ataatttctt
     6721 aggcatgacg ggaataatca ctttttccat cacatctttt tcgattttct tcgaagtgac
     6781 agcatcgctg tgttgggtgg aaactacgat ggtgtcaatg cgaataggct tgccgttttt
     6841 atattcgatg gttacttgtg attttccatc agggcgaacc cagggaagta tgttcttttt
     6901 ccgaacctcc gcgagtcttc tggtaagccg gtgggccagg ataattggca ttggcatgag
     6961 ttcaggggtc tcattcgagg catatccgaa catcaaccct tgatcgccag cgcctccaga
     7021 gtccactccc atggcaatgt caccggattg gttatgaatg gcggtcaaaa ccgagcaggt
     7081 gctgtaatcg aatccccatg tggcatctgc atatccaacc tcccgaatcg tttcacggat
     7141 aatctcagga atttctacat atgcccgggt ggagatttcc ccggctacaa aggcgattcc
     7201 tgttgtcaga atagtttcac aggccaccct acagttctta tctttggcaa tgattgcgtc
     7261 aagaataccg tcggaaatct gatcggcaat cttatcggga tgcccttccg tgaccgattc
     7321 cgaggtgaaa aggaaattgg tttgtctcat cgagttcccc ctaaccaatt gcttgagccc
     7381 acatgtccgt gccgggcatg gtctcagtgg tgaataaagc aaagcgcaaa acattcaatg
     7441 tagcgtaact taaccgcctt ccgcaatttt tgatattggc tatagctgaa tttttgattt
     7501 tatgctggtt aactccttgt tttcgacgtt ttcggtggga gaatcggtga aacgtgaaat
     7561 tctctacaac gagcagttcc cctaacccta ataaaggagt acaagggtaa accgggccct
     7621 ggcaatgaat agttgttttc ttttttatat gattttccag gaacacatat cgttctacac
     7681 gcacccatcg gattgtgaag gttccagagt cagccatcgc tcttcgaact ttttgagtct
     7741 tgcgtgcttt ttggtgggaa tttgtattag gttcgattag tgatagtgta gggcacaagc
     7801 attaactggt ttcatattga aacattagat tagaagtaag ctaagggaag gcaagtaatt
     7861 ttacgtggtt gacctgaatt tggtaagaac tgggaaaacc cccacagtag actttgaatt
     7921 ccactgataa atatatgata tttattatct cacccatgtc aaggtggaaa tgttcattgg
     7981 gttgcgtacc tagaattctc agtggtaaga tccgctctta tttcgtgtga aaaaacatct
     8041 gtttataagc atttcccggc gattgagacg tgggtgtgat gccgcttttt ctgaggcgga
     8101 aatctctaag gaggcttgtt gatgtacaaa acaatatatg tcccggttga taattcagac
     8161 cattcgaata tggctgtaga actgggggtg cagttcgcga aaattttcgg atcgaaaatt
     8221 gtgggtagtc atgtgtatgc ggcgaagatg catgataagc ggtttaagca aatggaggca
     8281 ggtcttcctg aggaatatca cgacgaaaag gagttagatc gccaacgcca aatacatgat
     8341 tcccttatta ccagaggttt gcaaataatt acagattcat atttggattt tgtcgatcag
     8401 aaatgcgcgg aagcgaacat tcctctggag cggcgctcac tcgagggacg aaattggaag
     8461 gcgattgcag aagatatttc aaaaaatggg tatgacttga ccattatggg ggccttgggg
     8521 gttggggcag tgaaggactc tgtgatcggc agtaacacgg aacgtgtgat ccgtcggatt
     8581 cgtaattcgg acatgttcgt tgtgaaagat accaaaccca tgaatggtgg gaaaattgtg
     8641 gtagctgtag atgggagcca ttactccttc ggcggattga agaccgcact cgccttggga
     8701 aaggcgctca ataagcctgt agaagcgatt tcggcattcg atccctattt tcattacgcc
     8761 gcgtttcaca gtatttccgg tgtcctgaat gaagaggctg gaaaggtgtt tcgatttaag
     8821 gaacaggaaa agcttcatga ggaagtcatt gacagcgggt tagcaaaaat ttaccagtct
     8881 catcttgatg tctctcgcga tgtcgcccag gatgagggcg cggatattaa gacaactctt
     8941 cttgacggca aagcgtttga gaaaatcatt caatacgttc ggaaagagca gccatggctg
     9001 ctaatcgttg gacggattgg ggtgcatagc gacgatgata tggatattgg cagcaatgcc
     9061 gagaatcttt tgcgttcagc tccttgcaat gtattaatat ctaacaagaa gtttgttcct
     9121 ccgattgaca cgcaggccga atacacgatc gcctggacgg aagaagcctt acggcggatg
     9181 gaaaagatac ctgtctttgc acggggagtg gccaagactg cgattcatcg atatgctatc
     9241 gaaaaggggc ataccatcat cagtaattct gtcattgatg atgcggttgg tgatatttta
     9301 cccaaagggg ccatggacgc catgaaaaca ttaggcggta ctctcgatgc tgctggagtc
     9361 gatcggaatg ccatgcaggc aggggatgag gtggcgcagg acttgatggg ttccaccctg
     9421 agcggcatga tgtctcaggt ggtagaagaa aagccgtcgg aggcttctgc gccaatgagc
     9481 gcagggaaca agtcgtatct agctcgaatg tccagagact attatgtttg tggtggatgt
     9541 ggatatattg ggaaaggcga tcaaccggtg atgtgccctg tctgtggtct ggatggagca
     9601 caattcaaac aagtcgataa atctattttt gatgcggctg ccaaggcgga aggtggcttg
     9661 gaaacccaag tatcatatga cgatattcct atgcagtgga cgaaagatgc tcgtgaagcc
     9721 attcgagccg ttcccgctgg atttcaacgc cgacgggcaa aagcgaaaat cgaaaaaact
     9781 gcaagaaaac ttggaatgac cacgattacg ttggaatatt caggagcaat gattcaagaa
     9841 gcggcaagcg aggactatac accaattttt gccaataaaa cggctgggga gacggcacct
     9901 atggtctctg aatccaaaac tgaaaatact ggcaataata atggagccga cccgtatgtg
     9961 tgggagcctg atgccctaca acgattggaa agagctcctg ccggatttat gcgtgattgc
    10021 accaaagcct taattatcaa acatgctgaa aaaattggta ctacaaccat cactctagat
    10081 gtggcgaacc agggaattga acaagccaaa actactatgg aagaagccat gaaaactggc
    10141 aatgtcaaag acatcgtggc caagttgact ggcacagggg ctccctcgga agctcaatcc
    10201 ggagccggac ataatgggta agtctctgcc tgttctcaat tcattcctta acacgctgaa
    10261 ttcgttaggg gccggagcaa tttctaagtt tgaatcattt cgtccagact ccggagcgcc
    10321 tggggatggt cgaaccgtgg atgattttaa gccctacctc gtggctctca atttaaccaa
    10381 gcgttgcaat ctcaaatgcg accattgtta tctggatgcc acaacgaaaa tgggtggtgg
    10441 gcaggatgaa ttgacgacag aggaatgttt tcagcttatt gatcagattg cccaagtcaa
    10501 tgccggtagt cttcttgtta ttaccggagg agagccgttg gttcgtcctg atattcttga
    10561 aatcgcgcgt catgcagtcg agaagcgatt tatggtcgta tttggcacca atggtatgct
    10621 gattgacgat aagatggcga aagccatggt agacattggt gtgatgggcg taggaattag
    10681 tattgattcc ttagattcag caacacacaa tgcgtttaga ggtttacctg gcgcatggga
    10741 aggcgctatg gctggcattg aagcctgcaa acgaaacggt cttcaatttc aagtacattt
    10801 cagcgcacag cccatgaact acaaggaatt accagcggtc atcgattggt cacacgattt
    10861 aggtgccaaa gtcttaaatg tctttttcat ggtgtgtact ggacggggtg aagaactcac
    10921 tgacatcact ccttcgcaat atgaagaggt cttgacttat atggttgaga gccaagataa
    10981 ataccaggac atgttggttc gggcgcgttg tgcgccacat ttcaaacgct tggcctacga
    11041 aaaggaccca aattctccca tcaccaaagc gacaggatat atgggaggag gatgtctggc
    11101 aggcacaaac tatgcgcgag tgacgccaaa tggcgatctc acgccatgcc catatatgcc
    11161 cctttctgca ggaaacattc gcgacaccag ttttgtcgat ttatgggaaa attcagaagt
    11221 attcaactcc tttcgctatc cacacctcaa gggtaaatgt ggagattgcg aatacagtga
    11281 aatttgcggc gggtgccgtg ctcgtcctta tgtggaccac ggggatgcga tggatgagga
    11341 tgaatggtgt ctgtacactc ccaaaggggg agaaaaaatt aaagtggcct tcaatatgcc
    11401 ggaagcctcg tcggtggaat gggaagccga cgcagaagaa cggctcagtc ggatcccata
    11461 ttttcttcga gccatggtca aaaaaggtgt cgagcgtcat gccgttgaac aaggtcttcc
    11521 aatcgtgacc attgaattaa tggaagaact tcggaagcgt cgattcggaa atgaaaaacc
    11581 tgtgtttaag ttctaatgga gtccctcctc ggagttctca ccgatcttaa tcagattatt
    11641 cccatcctgg tgcattggtt gcacctactg tctgccgtgg tgtggattgg tggtttggcg
    11701 tttcttgtca tggccgtaac gccctgtctc aaaaacactg ttccaaaaca atttatccga
    11761 ccaatatctg aaacctttta tcagcaatat aaacgggtgg tcgggattct cttggtagtt
    11821 atccttttta ctggaggatt caatctccat tatgtcagtg aggggatggt catgcagtct
    11881 ggggaaggtg tctcccatca tgccaaatac cttactgttt tctttattaa attgttcttg
    11941 gtgctaggaa ttctgaccat ctttctatac accattatct ttaaaaatga acccacaggt
    12001 ggtgagacaa gcgaagaatt agaagagatg gccatagagc cagttccatt tcagcgtgta
    12061 gcattgtgga tgggggtgtt tatcatactt tgtgcggcag cgatgaaaca tctccatgtg
    12121 taattaattt ttttacattg aattttgcat ggcgtagttt tagaggcttt tcccctgctt
    12181 caatcttctg aataattgaa cctaccagtg ggattggctg ggttgaccac aaaaaagcct
    12241 aaccctttcg aatacctgtc atagttgata ccaaatagct aaagaattcg aagggatcca
    12301 tttctcaaaa tggatccaac tgcagaaaag atcttcctcg ataaattacc gtgtggctga
    12361 ggaaaaatca gggtcttaat gaaggttaat ttccagatag ttgactgggt agctttatcc
    12421 aggctatcac tgggcgggtt cctttgataa atcgtcttgt tgatctggta gacgaccgga
    12481 cgcctgaaaa ttaattttcc taccaatttg ctccacccta ttaggttatt tgctgtccaa
    12541 tggctcgatc ttcccgttct cgcacccctc gctcccgtac cagaccatct ctggaatcgt
    12601 ccctaccgtc tgatctgcct gtcgaagttc ccttggaacc acccccacat agaccattgg
    12661 tagccctggt gggacggccg aatgtaggaa aatctacgct gtttaatcgg attgtcggcc
    12721 atcgcacggc aattgtagat gatgtatcag gggtcactcg ggatagaaac acggcagagt
    12781 gcgattatca aaatcggata ttcacgttgg tcgacaccgg tgggctggat ctgaccaccg
    12841 acgatgtcat tgtggaacag atcaaatttc aaacccaagc ggctattgag gaggctgatc
    12901 tgatcattgc ggtcatggat ggccgtgtgg ggttgtctcc gctggatgcg gaccttgtta
    12961 agctattgcg accggtgacc aagcccgttt ttctggcggt caacaagatt gatactcctc
    13021 aggctgaacc gctcttggcc gatttctaca aattaggggc aaaggagatt ttccctattt
    13081 cggccgaagg aggattagga gtggatgaat tactggaagc ccttttgccc tatcttcctc
    13141 aagccaccga gaatgaagtc caaagagaga tcccaaggat tgcggtggtg ggtcgaccca
    13201 atgtgggtaa gtcgacgctg gtcaatgcca ttctcggtga acaacgactc attgttagtg
    13261 atatcccagg aacgacccgc gatccaattg attcaatggt cagtcttcat gggcgacggt
    13321 atatctttac agatacggca ggtctccggc gaagggggcg gatcgagcgt ggaattgaag
    13381 gctatagtgt cgtgcgagcc attcgagcgt tgggacgatc tgatgttgcc gtgctggttt
    13441 tggatggggt agaaggagtc acagaacaag atacgaaaat tgccggctta atcagtaagc
    13501 aagggcgagg ctgtattatc ctcgtgaata aatgggactt gcgcgcggat gatcgtgagg
    13561 cgtaccccgc ctacaatctt gaattagctc ggcggtttcc attttttacc tatgtgccgg
    13621 ttgtctttgg tgccgccatt caatcggaaa cgctcacgcg aatctttcca aaaattgatc
    13681 aggtagtggc agatttttcc aagcgtattc agacccgtaa gctgaacctc tttgttcagg
    13741 aacttctgga aaaaaatccc atgacgttgg tccgaggcaa tccaaaaaaa tccgtattta
    13801 tcactcaggt cgcgaccaag ccgcctacct ttgcgttatt tgttaaatcc gcaccaaaag
    13861 ttccagcctc ctacctccgt tatctagaaa actccatcag gacggaatat ggttttgagg
    13921 gcattccgct ccggatattg ttgcgcagtc aataaaaaat aaggaacact ggaaatcacg
    13981 cgagttccct aagtgaagca tcgtgagtca gacaggctag aaatttttga agttcagaga
    14041 ttcatgcagt acaatttgac ggatgaatcg gttgctgtgg atatgcgtcc tgatcctttt
    14101 aggctttacc gcttcctatg agacaaggga cgttgaagcg caacccgaag ggaagcaggg
    14161 gcgagacctc tatcgtattc attgtctgga atgtcatggg gcggaaggac ggggtgatgg
    14221 tccacgggcg gccctgttgg cccctcgccc cggaaatcta gtttcggcag ccacctctac
    14281 caaaaccgat gaagaactcc ttgagatcct tgccaatgga gttccccgaa cggccatgca
    14341 tggctggagt gaacaattgt cagaagagca acggcgcaat gtcctcgcct acgttcgaac
    14401 cctcgtgcat tttcatgacc tatcttctcc tccttctcca ggaccttagc ctgttgtcgg
    14461 tttgaggcgg gtcttggatt tatccggtcg catccgactc aagtgatcga agtagtgcac
    14521 gaaggcttgt atcccccctg aagcttgtcc ccaatcgaaa tattcattgg gggcatggta
    14581 cccatgttcg ggtaggctga gacccataaa cagaatcggg accttccagg cgcgttgtaa
    14641 caccgcaatg gccccaatag atccaccttc ccgcacaaac accggtcggc gtccaaagcc
    14701 ctgggctacc gcctcttgca gcgcctccgc ataggggcca tcggaaattc cacgataggg
    14761 ttccagttct ccatcattaa tcacttcaat atcaggattg cgggcacgaa gatgtttttt
    14821 cagaagtcgg aaaatttttg agggatgttg atttggaact aatcgcatac tcactttgag
    14881 ttcgccccag gccggcacaa tggttttgac gcctggtccc atgtgcccac ccgttaatcc
    14941 atgcacttca aaggtgggag aggcccagat ccgtttgagt aaatcttgag gatcggaggt
    15001 tcgaagcgat cgcagttgat aggcctcttt aaaaaggcga acctgaaatc ctgatgccag
    15061 aaaatttttc atttcggttc gggtgggcgg gacgacatca tcgtagaatc ctgggatttt
    15121 cacgcgtccg gttttcgcgt ccaagcagga ataggcggcg tcacataatt cagccaaggg
    15181 attgcgcgcc ccgccgcccg tgagaccgga atgaatatcg gtttcccctg tgcgaagcag
    15241 caacttggcg accaacaaac cacgaagccc acaggagagg gccgggcgat ccttcgccac
    15301 ccaaatcgtg tcggacacca gtaccgaatc agacggtggg acagctttac ggttttgaag
    15361 gacctcgggg aaattcgggc tggcaatttc ttcctccaat tcccagagga tccgaatgtt
    15421 taggggaatg ccgacttctc ttgcgaattt tgcagcaaag agggcggtta acgccgggcc
    15481 tttatcatcc gttgccccac ggccacagta ccgatcacca tcctttttaa aggcaaaggg
    15541 agactgacgc cattcgggtt cttgagccgg ttggacatcg agatgattgt aaatggtcag
    15601 actggggtgt tgtcgaccgg ccacccattc gccagctacg cttgggtaac ccttggtgtt
    15661 cacgatagag gccttggctc caaattgttc caggtattga acggctaatt gggccattcg
    15721 gtgcatatcg tgggcatgct cagggtctga acttactgat ggcacttcta ccatctgacc
    15781 taaaaggtct tcaaagttgg ggcgctgatc ttgaatgtag gtgtgtaatt gggattgata
    15841 atcactcatg agatgacggt tccttttcgt gagtgggctg agagtgcaag tacgaaaacg
    15901 agagtcggga atacggatgg cattccaaga acaacgacaa gggggctagc atgtcgtcat
    15961 ccaactgaaa acggcaccgg agcaaaagtc acaatgaaaa tagccaaggc cgcccaggcg
    16021 acccatcgcc ggtgtttacc caaaaccgtg tgcgggtcca tcactggcgg gtgggcaatc
    16081 ccgacaaggc tggccaatcc gacccagaga atccacccag gccaacccgt gaggcccaaa
    16141 taaatgagga tgggaatcgt gcccaatgcc agggagcgtt gtcgacggcc taataccgca
    16201 aatgccacat ggcctccgtc caattgcccc aagggtaata aattaatcgc ggtgacgaaa
    16261 aacccgaacc acgcggcaaa agcaatcgga tggagcacaa tatcgtgcgt gggtggaatt
    16321 gggccaaaca tgagccaggc aaaccattgc agcaggagag gttcgcccaa ctgtaaccca
    16381 tacgtttggg atctggggac gatatgggag aaagagaggc cgataataat ggcaacaact
    16441 gcggcgataa atccggcaat gggacctgca acgccaatat caaaaagcgc gcgacgattc
    16501 atgattgcgg atcgcatgcg aatgaccgcc ccaaaggtac caatgaaatg tgggggaccg
    16561 ggaatgaaca gcggtaagga cgcgggaaca cgatggatgc gggagagaag gtaatgcccg
    16621 aattcatggg tgacgaggat tcctaatagg gttgcggcaa aggggatccc attgactaaa
    16681 gagccgggat aacgcattaa aaaatcccag gccccgttga ctggtttcat attaacctga
    16741 aatgctcctg cccagagtgt agtaaatatg gtgagcccaa agagcaaaat aggaagtccc
    16801 cattgggaaa aggcgctggg agttggaggt gggaattcat ccacctcatc tcgagagtcg
    16861 agccggtcag gttgaattga atgccaatga cctattccgc gggaggatgt ttcgagtgtg
    16921 cgttctcgaa gcatggcgtc tttggagatt ggcatgatca gaggtggagg ggaaattggg
    16981 ttagttgaag gcagtgaatc ggtgggagca ataggattca atagagaagg tgggggtgtt
    17041 gggttgttct ccgaaggcgt tgaatgtgta ggcgattcgt gatccattaa cacgtcctta
    17101 ttgaatgcca agccaggaat tggtctgata ttcaatatta ggcaaatgga ttgtgcgctt
    17161 gttcctcaag cactgtcgtg gagggaccat ggccaggaaa caaggcggtg tcttttggta
    17221 agtgtaacag ggtatggcga acggattgca aatgccgtgg atacagggaa aaagggttgg
    17281 agcgccctac cgacccggaa aagagcgtat ctcccacaaa acacagggtt tggcctttga
    17341 gcgataacag gtagcaaaat cctccagggg tatggcccgg ggtggccaag cattcaaccg
    17401 tcaaatcccc cagggcaatc atctgatggt cggctggtat ttttattgag gcgtcaggcg
    17461 gcgtccatgg gagcaggtcg aaatcgccat tacccagata gaccggcact ggccattgag
    17521 ccaaaatctc tttcagccct cccgcatgat ccgtatgccc atgggtgagg cagacgccga
    17581 tcaaggtcaa ttcacgttgg gagatgctcg aaagcatctg tttggcattg tacccagtat
    17641 caatcataat ggtctggttg gtgaccggat cagttaatag ataccctttg acttcatacc
    17701 cgccaatatc gcctttgatg gtctgaacaa gatcgtgttc agtcgtccac tctggttgcg
    17761 gttgaggtaa ccatccgtcc aaagtcaggt taatcagggg tccaacgcgt aggtgcaagg
    17821 cctggctgat tccatgaatt tcgcttttgg tcggagttcg gttacctttc tcaaggtcct
    17881 ccatgtcggc ctgctccatt ttcgccaatt ccgccagggt ctcaatcccc agcccttgcc
    17941 caaaacgagc ctttttaatg atatcgcaaa attcatcttc taaactcatt tacatgctca
    18001 aattaaaatt ggctatggtg atggaagttc cctctcttgg cttgctgctt catctaatca
    18061 aaaaagaggg gcctggggaa agggctatct gtcttttttt gaatcaggac ggttgaccca
    18121 tttgagatgt tcttgtgaga ggggcgtatt tttcgatgaa aaatgagtag gggttcattg
    18181 cttcacataa ggcatttctc caaaatggag agcgtgtcaa cgcttccaca agtcatgata
    18241 ctttgcaaat accgatcctt tatcgaactc tggggtggga attttttctt gacattaata
    18301 ggggaatagg ctacttaaat gaaaacaatt atctgggata aagtgccact tacaatccaa
    18361 taactttaat gggaggcagg aaatgaaaaa tctgctaatt gctggttttt tagggttttg
    18421 tttactcaat ttgaccggat gtatgggggt agcctcacct gttgcaggat gggcctatac
    18481 tgaagctaaa ttcggcaacc aagttactga tggcgctgct gggactaaaa ctggaacagc
    18541 ctgtgcaact tcaattcttg ctatggttgc gacaggtgat gctagtcagg aaaaggccat
    18601 ggcgaatggt ggaataactc agattaccta cgttgatcat tccgcaaaaa atattcttgg
    18661 aatctggggt gagtggtgca ctatcgtgca tggttcgtaa tcggttagta aattaatttt
    18721 tttgtgggat tgtcctaagg gtgacttagg acaatccctt ttttttcccg ggaaaagtag
    18781 tccaaacacc tagacctaga tagtgatggt aatttttatt gcataggcca gtttctcaaa
    18841 ctataaagta catgcctcta ttttcagata ggctctttcc agcccaaggg gaaagcctgt
    18901 ttttgcatta aaatcttcgt ggccatactg tgggataatt taattgacga ggttcctctt
    18961 cgctctgctt tgttcttgta gaaactgctg aatagccaat tctctccccc tactggcttt
    19021 tcgtaaaagc cataggacta aaaacctccc gccccacaac ttttttgagc gtcagttccc
    19081 ctcgaggccc agtttggtcc ctgatactct ggaaaatgtg caccatttgc caaggcaaaa
    19141 accttcggat cggcccaaca cagcagatgg acgtaatccc tcactgccta ggcgggcaag
    19201 ctctatgtcg tagggttcgg caaggctgag cgttacctcc gcattgacgc caatcagatc
    19261 atcatccaac aaagaatccg gcggtttttc atttggctga agaatataat ggtgaaaaag
    19321 gatgggacca aaagaggcga atttcatttc ctagtgtgtt ttcttgacat attaggcaag
    19381 agcacctgga atataatttc aggcgatcta aggtttctta agcatgttga tttagaattt
    19441 actcggagag agagttgtat ttttccataa aatttgtatt gctcctttcc tgggttgtgg
    19501 caatttttct tccctgtcgt ttggaggctt tggatgacat tgagatttat ttggcgggct
    19561 atggtatggg gtcacaacct ttaaataagg gagtttcgtt taaaagcaga gaggtttcag
    19621 gcgagaagct gaatggagat cctggtttgg gatttaaggt agggttcttc cctcattttg
    19681 ccagtggatt ccttggtctt gaattggaat catttggaaa taataattca atccgttttc
    19741 ccatagacag tagtttgaga ccgacaaagg gtaacagcag ccttataacc tatcagtcca
    19801 tggtcaatct tatcgtgcga tatccaggga ctctgttccg accgtatgcg ggcataggag
    19861 gcgggctttc aaacggaatt ttaagaaatg ccgatattcc aggaagaaaa gataaaaatt
    19921 ttgaaacagg aacggctcct ggatatcagt ttctaggagg catgcaacta ttcattaccg
    19981 aacgatggtt tgtatttgga gaatacaaat atttctctgc gaattaccat tggaaggaac
    20041 tttccttaaa tttcaggagt gaatattttt tggggggcat tggttttttg ttttagattt
    20101 tatgtttctg ttatagcttt tgcgaagtaa ggcccaggca ggcctgggcc ctttgggagt
    20161 tagaaatatc cgtgaagaac agtgagaact tcattgtcac gatcatggtg agtgaacgtg
    20221 gcgcgcaccg cgaaattttt ggcgaatgtc catcgttgtc ccacagaaac agggatgtca
    20281 tgcgatcgat ctccaagggg ataatacaaa tatcctgctg aaatgagcca tttccatctc
    20341 ggagtgattg tggccatcac gccgccagat attcctcccc ccactcgatg attttctttg
    20401 tacgcgccac tgacgttggc atctatctcc ccaaggagaa aaaagacctc ccgtttaaag
    20461 atctgagttt cttgggctcc accgattcct gcattaatgt ttccgttaga acagaggtcg
    20521 caacttgagg tcttgacggt gtccattcca acattaaatt tccatgaggg cgaaatgaac
    20581 aaggaatcaa tgggggccaa ggagaggata tttgcaaaag taaaacgttc tactcgaaat
    20641 tgatttcggc tgtgataatg cctcaaccgt aaatcaagta attctatttg ggcatcttgg
    20701 gtatatcctg cgtcaggatc taggagatcg tgataggcgg ctcggacgct gatttcttcg
    20761 aatatctcat ggtttctcca cccgaacccc tcgccgactc ttagtgaatt gtgtccgtga
    20821 tcgggggaaa gggaaaatgg ctccacatga aaggagggcg aaggtatttt tatttgactg
    20881 cgcgtcacta atatagatcg atttttggcc ttatagtctt cagccttgtc ttgatcggtt
    20941 gcggctttat atcgtaatac gtctgatgcg aaatccagaa gaaaaatttg gcgattcctt
    21001 ggtagttggc gaaattcaaa tcgctctgtc aaatgaggat cgttgaggag tttaggaagt
    21061 aacgaggttt cttgtttctc caaatcgctt cgctttcttt taagcagagt gctgcgagca
    21121 ggtcggtaca cgctctcttt gaccaatcct ttgtaacgaa aaaggaggcg aatggtgttg
    21181 cctggaatcg tagcaaaatg aaactgatcg gtcagatgca gattgggatc ggccacttcc
    21241 aataaagaca ggatatggta cgcacaattt tctttaaaga agtaatagtc gaaataggca
    21301 ttccctaatt cccacacatg catcaacagc cgatgaattt gatcgggaga aaaattcaag
    21361 cgatattccc agatgtctcg attttcaatg tctccgtatt ctttgacttt catataatag
    21421 ggaatagtcg aaaaaaaccc tttgtatcct ccaaatactc ctttgtaagc gtattcaatt
    21481 ccaggattgg gcggcacctc tgccgcatag ttaatcgtat aggcgagaat ccgtgtttgt
    21541 tcggtttggc cttttggatc aactctgagg aaagtgtgcc caaacatgga agctgggttc
    21601 cccataaagg ctgaggggaa aatcatggaa ataaattcgg catcaaattc atcataccaa
    21661 tgatgaaacc gttcacattc gtgagaaggg agtttagcct cttcaaattt taatttttct
    21721 ttcagccaat gatagcgggc gacaaaggcg cactgggcag gttgttgtga ccgaccgacc
    21781 aatttatcag aaaaaaagaa ggaaattgtt gcccgtaact cagcctcggg atcttctttc
    21841 ccattttctg ataggaaaaa ccctggatca tccacttcgc tggtgtgacc accaaagagg
    21901 cttggctggt aatgaagtaa gaggtgccag tagcgttgct catggagttt gtccctgacg
    21961 gcttgctcat tcaattcttg aagatacagt ggggttgccc cttgtatggg ggagacaaaa
    22021 aaatggaaag aaaatagcca aaggccaata agaaggcgta ttaccacatg cggggagaga
    22081 aaattgagag acgataaggc tccgcctcta agagccggag cctttcggaa tttacttgtt
    22141 ggttgtggtt tgggctaaaa taatgtggcc ctgcatcgcg ttatttattg ctttcacaac
    22201 ggctttcggt gaactttctc cggcttgcac cagagtcgaa tatttctctt gcgtcattga
    22261 aaaaaactct gggtgctgtt cttgaggtat ccccattaat gtggctagtg atgtcaaatg
    22321 ttctccttgg ccttgcgcca tttcttggga taggttgtcg aaattcaggg ctgtaaacac
    22381 attgactttt tcggatgcaa aaattttccc atcattggtg caaccggaag tgcctgaact
    22441 gattgcaaaa gtttgaaagc cggtaccatt ggtggtggcc atcaggacct gaccaccaat
    22501 gctttcattt tggctggact gttcccacgc gagtttccct aatccgcaac ctggaccatt
    22561 gtttggggga ttccctaacg ccatgcctga cattcccatg accagaaaaa atgttgacac
    22621 caatgccaat ggtttttttg ccatgctttc ctcctaattt ggtggtgaga gataaaggaa
    22681 ttgttttacg acttgtgaag atgattataa caaatggaag gctattggaa agtcaaaatt
    22741 tacacaaaca tgttcattcc ccattgacgc ctgacgggag cccctctggc tagtttattt
    22801 tgtgttcaga cttcagtggt tgaatattcc ctgttggtca aagcaggaat atttcgtgat
    22861 gcgtcagccg gagaatggtg actggttgtg gataaggaag gaattctagc tggctttaga
    22921 tccgggcatt tctcgatgag ttgtctgagc gttcgatggc cttcttcgta gccagggtgg
    22981 agggtgatga tttctttcat ggtggcaaaa gcttttggta gctgtccggt ctcgattaat
    23041 aatgctgcca accgtaacct tccttctaaa tttaccggat tcttattgac cagcatttgc
    23101 aacgtctgaa ttccttcttg atagttgcct gcatccgcgc agtggtcggc ataaatgcca
    23161 taaagatggt tatcccattc agagacaaag tgcggttgtt ttaactcctc ccagtccatt
    23221 gggacagggg ggcttattgg accgatcttt tgtgccagtc ggaatcgatg gctgtgtggg
    23281 tcagataatt ctgtgaattc gggatccgat gatggatgga aacctaggcc gttccgccta
    23341 aggctttcct caatttcctg attggaatac gaccgtatcc caatggtgtc ctcgagagga
    23401 acgtcttgaa aatggcctat gggcagttga aagaccagat accccttagg agtcaagacg
    23461 cggtggattt ctctcaggta ccgttcaaat acaggacgag gcatatgctg aaatgtgaca
    23521 taggaataga ctagatcaaa tgaggagtga ggaaattcca aaagatccac cccagaggtt
    23581 tcatatgtgt ggatatgggg aaagtcagct aaccattgtt tgctctgggc aatcattgcg
    23641 gaagagacat cgacacctac taaatgatga aaatgggagg cgagggcttt gaggactctg
    23701 ccaatgccgc atccgatatc taaaacattc cacgacggca ggtgagggac tgggagagtt
    23761 gccagcaagg cctgggcagt gttttttcct gattgagtaa acatttcctc tgtgtggtaa
    23821 gcctcggtgg caatccaaaa tctggcatgg tgttgggctc gttcgttcca gtcatgtttc
    23881 attttttccg caatgttcat aggtttcctc gcctccatca gttcgagata ttgcacctgg
    23941 tccaatgttg aactttttct ctgtgtgctt gggtatgttg atgtatccgt acgaaaggct
    24001 ctgcttgttt aatgataagc tttccatgct ttgtcttgtg acaaaaggtt tagcaagtcc
    24061 gttgccaact tcattggaga gggcatgtca tatatcgggc tcacaatttc cagaatgtgc
    24121 tggtgaaatg cgcatttttg agagagaaag agtttattca atgtggagaa ggcaatgggc
    24181 aagagattct tgtgagtgga aaggtatgcc gagttttaaa atttaatgac taggttaatg
    24241 tacttgtttg cagatatttt cgatcagatg gtggatgaac tttagtaggg caggctttgt
    24301 aatggggcgt caataaaaaa cgggacttcc aaggtgagag aaatatgaag atttgagcga
    24361 ggagtgaata agtgggtact aagcgaacac atcaatacga gaggatatag catccgttgg
    24421 tttttcagac ggaatcccgt cttctttggt tttgggtgca tttttttgcg ggctgccgct
    24481 tcaatggtat tggctgaccc gtattggctg acacggccac cgtccgatcc tgggctgaag
    24541 atctgcaggt gctagggctg ctggtcgaat ttactgggct ttttgtatgg tggattgtgg
    24601 attacctggg atgagaaaag tggcaatact gacttcacct ccgaccgcat agaatttccc
    24661 atcagaccct cgttgaaagg aaaatctggc cgcacttttg gctagtcttc ctgatgctgc
    24721 tatgtgtgcc tattggttat ttctaacatc acgatcgcta ttctggagtt ccacgatttt
    24781 tttttgtatg gagggagtaa cttgtgattg tttggccttg tgcccgtcag aatggttttg
    24841 gagggctaat agagaattct ggggtgaaaa gggtgaggtg gaagaggata tgttcattag
    24901 ggaaagcctg ttcacattaa tttcggcttt tttggggaga taaattgact catttttggc
    24961 caatggtggt ggattaatcg tataaataga acgcctaaaa aacgttttgc gggggtggcg
    25021 cagaaaccgt gatgtccgaa aaatggtttg gatatatcgt cctgaaaacg gaagcaggcc
    25081 atccctttcc actctaatgg ccgcgtccca ggcggttctt aggtcaaaaa cctactacca
    25141 cgcgttcatg cttcggtttt agaatgaaga attggagacg ggaacgggag attcaattgt
    25201 tcctttctta tttttccggt aagacaatgc ctattgagaa actaatgaaa aaattaacgc
    25261 agattcattt tcctattgca caaatggcag gcttattaat tttgtctgag agtccgcttg
    25321 aaggtctcaa gtggaatagg gctcccgtgg ccgttgtttc accttaaatt gttttgcttc
    25381 cgggggattg aaggattttt attttctaac agtgccggcg caattgtttt gtgatcttct
    25441 ttgtgtccaa aacaggcttt gcgggccata gagtcagaac cgcaaggaag atacttgaaa
    25501 aaacaagtag tagaagtgtg gtggtggtgt ctggtcgctg gatctgttcg aaatataagg
    25561 tttgcgcatt tgccaaaacc ttgtcagcag cagatacccc gcccaagagt atcatggtca
    25621 tgagggaccc aataatcatt cctccgaata ttttcagcat gagatcactc cttttgttga
    25681 agacgaaggt caacgagaaa aattttcaat ctcttgggtc aacctcttcc caaggcgggg
    25741 aaaggatttg tgttgggaag taaatccatc cccctggtga agagatatag agaaaaaggt
    25801 taatgggtaa ataccgctaa aggattggaa gtctagcact gattaggaag gctatagagt
    25861 taagggggag cttttttctt atcgaggaag gggagaatac cgttttaagg agaagagcag
    25921 gatggcaaag accagactta ttggtctaac gctagctgaa ccatagacag gaactgacta
    25981 aaattacagg gtttatctaa aatagcaaag actcccgcct gacgcgcacg atgtttatcc
    26041 tgttctttca gatgtccact gagtaagatg acgggggttt ggatgggttc tttctctttc
    26101 agcttgattt ggtcaatgaa atctagtcct gccagaaccg gcatttgatt gtcggaaatg
    26161 attaagtcag ctcggtgagt ggataaccac gcgatggcct ccgatccatt gctggcctca
    26221 atacattcta attgttggga ttccaggact aggcgaaggg cttcccgagc attgagatca
    26281 tcatctacca gaaggacctt tttcatgttc ttgttcgctt atttaacccg ttcttcaatc
    26341 ttgagctatc acctcattat tctaaatgat cagaacatca cattgtccac cccccgaaga
    26401 gactagccgt ttttttacga aagctccaga gtttatcctc cccaggatta ttttaaaacc
    26461 gagttttgga tttttcgtta ttgagaagaa agagggatag cc
//
LOCUS       DAORAQ010000025        26153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_359_length_26153_cov_62.628017, whole genome shotgun sequence.
ACCESSION   DAORAQ010000025 DAORAQ010000000
VERSION     DAORAQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 26153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26153
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_359_length_26153_cov_62.628017"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /gene="secA"
                     /locus_tag="PKM72_04110"
     CDS             complement(<1..1588)
                     /gene="secA"
                     /locus_tag="PKM72_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNP60000.1"
                     /translation="MFVRLLNILFGSKNDRELKRLRPQVIRANEFEASLQPLDDEGLA
                     AKTAIFKKRLEAGESLDDLLPEAFAVCREMSKRQLGMRHFDVQLLGGMVLHQGRIAEM
                     RTGEGKTLVATLPVYLNALEGKGVHLVTVNDYLAKRDAQWMGPLYHGLGLSVGIIQHD
                     ASFLFDPTYDAPDKRLQFLRPCSRREAYGADITYGTNNEFGFDYLRDNLVVTDLSQGV
                     QRELNFAIVDEVDSILIDEARTPLIISGPAEQSTDLYFQVDRIIPHLKREEDYTIEEK
                     TKTVSLTDEGNAHVEKLLGVDNLYDLKHLSLVHHVIKALQAHVLYHRDVEYVVKDGEV
                     MIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYNKLAGMT
                     GTADTEAAEFAKIYNLEVNVVPTNSKMIRIDNPDVVYRTEKEKFEAIADEIKEYHEKG
                     QPVLIGTISIEKSERLAALLKHRGVKHHVLNAKFHEKEAEIIAQAGQKGSVTIATNMA
                     GRGTDILLGGNAEALFKQQVI"
     gene            1861..2154
                     /locus_tag="PKM72_04115"
     CDS             1861..2154
                     /locus_tag="PKM72_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60001.1"
                     /translation="MPYRKSLTVVLILMLFLIMGSMHRTYASHITEHTHHHTSAMHST
                     AICSWMCAAAQTISGDNQEISPSFQLLAIMEYPIPKVPTLISTHFLPTRAPPR"
     gene            2478..3011
                     /locus_tag="PKM72_04120"
     CDS             2478..3011
                     /locus_tag="PKM72_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNP60002.1"
                     /translation="MASNQTSLAENLGESTEPIETLDVIEVTGTTVTKTSRTFSFPLP
                     TIDRPWPPTTFHTLGAPDLDMDNLPQPAIPRILLDRIGTTRGRLKSVKPLKTERPLYP
                     RMAREQGWHGKVVLRTHISSDGTVTSATVKESSGFSLLDESAVQSVKGWSFEPAKNGE
                     FTVASTVDLPIRFDLLQ"
     gene            3049..4101
                     /locus_tag="PKM72_04125"
     CDS             3049..4101
                     /locus_tag="PKM72_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP60003.1"
                     /translation="MIPPFSKIRLIAFFLLVTHIGLGSPSSQAGEAQDLLSQAFSSYE
                     QQQWTSAIASLERALALYPGYAEAHHLLGLIFNNLEKPDKAIMELQRAVEAYPKFAQA
                     YVDLGSIYQHQAQFPKAEQSFNLALKAYPKFTEARIALAALFDQQQQAQKAIKAHQAV
                     LELEPHRVDSLYGIAFWLAQEQKPDEAQRYVDQLVSLHPTYVNGWLMAGSLAQQSDHV
                     DNAIQAYQHAVKLNNDLASAYFNLGILYRQKEDFKEAAQAFEHVTALDKNNAEAHVNL
                     GVVYAALSQIDQAEEEYQTALSLNDQLAEAHYNLGMLYEFHRNAPQKALKHYEEFLKL
                     GGQDERIQTLLQRSKS"
     gene            4098..4988
                     /locus_tag="PKM72_04130"
     CDS             4098..4988
                     /locus_tag="PKM72_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNP60004.1"
                     /translation="MNSTFEDSSSNFNNRIFLLSALGSVILHAGILAALTYYPSPPEV
                     KDLTPTVQVTLVPALQEISPTPQPPTKPLTPPQPDFAQPTPPSPRTQPTPAMPATPMQ
                     ASIKPTPSKPLAPPPLPQAKPMLKDTRTVQAIQSRQMMKMAVPPQHRQRPQLSPATPQ
                     REQPPARTPMHMNNRQPIAQHTLPPPPNPSARPALKTPPAMAAGSSTSKPRIVASTKP
                     LYPRVARESGWEGTVIVRTLINTDGAPSQVEIRKSCGHEALDLAAQEAIKNWKFQPAK
                     DGNIPIAKWVDIPIKFDLNS"
     gene            5016..5603
                     /locus_tag="PKM72_04135"
     CDS             5016..5603
                     /locus_tag="PKM72_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNP60005.1"
                     /translation="MIQLLMGGGWMMIPILGCSLLALTVSIERGFQFRSLRFSRLADR
                     MIKLVEQGKWTDALALAEQRQSPTLRVLAAGIFHRDQQPDKAMEAAGIGELARLKRGL
                     SVLDTIITLGPLLGLLGTIIGMIDSFDIMSESGLGNPHAVTGGVAEALICTAAGIGVA
                     VITLIPYNFFLSLVERETERIEQYATKLQSFLGHS"
     gene            5566..7185
                     /locus_tag="PKM72_04140"
     CDS             5566..7185
                     /locus_tag="PKM72_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNP60006.1"
                     /translation="MPPNFSRSWDIPNSHIQLTNGMDNPEQQSREIVAKLRRFIHLAC
                     LKPPGMIIQGERKWILSQQAPYTLLPLTILLLISGLGLMGCSIPTHSPPTTNIESFDN
                     RKTAKTYEFINGQWFDGQGFTRDTWYAVDGSLTRLRPNVVEQTIDLKGGFVVPPFGEA
                     HTHNVEGAWNIDQVINHYVRDGIFYVKNTTDIPEFVEQIDGKLNTPETIDAIFAHAGL
                     TGKSGHPIALYEDILRTHRYEPVIGKRKKGWFNGRAYFALSSLKDVETYWPAIMATKP
                     DFIKLYLADSKHFENGTRSTTHGFRNGLNPQLIEPIVTRAHQQNLRVSAHIETVDDFR
                     TAIKNHVDEIAHLPGWFLPSPDQESAVRLTQEDAQLAATHRTVIVTTTVAEHFHPAGH
                     HTKTDAHPHSPANSQEHQPPPVKNVLEVAKRIQMENLKLLHQAGVTIAIGSDHAETSL
                     AEALHLHHMGIFDNLTLLKMWSETTPQTIFPSRNIGRLDEGYEASFLVLQGNPLEDFE
                     QVQNIILRMKQGSPLSWSETHHTDATAKHAH"
     gene            7697..9916
                     /locus_tag="PKM72_04145"
     CDS             7697..9916
                     /locus_tag="PKM72_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNP60007.1"
                     /translation="MIDLKYLKIKNMQLALQFCAAISFLLIEPSLGIAEEIIETLPTV
                     TVEAKRIENIDVVKEELARRPSSTILIEEKEIEESRAFNLTDVLQFAPGVRFQSRFGA
                     DEGQFQIRGTSLRNNFHHRGINILINGIYFGDADGFSDFESIDLMAYERIEVYKGSNA
                     LRYGANTLGGAINFVPRTGYNASLYQARGLAGSFGLYSGQVSSGQVTKPFKVGTMDTT
                     ADYYISASGNHQDGFQDNSQQARGRLNANFGFQIGDHQEVRAYILGAEVSEALPGSIT
                     QAQLDSDRRQANTLGGINPFSCGPNQACKWGRYYSYIRVGAAYRNEFAAHQFFEIIPY
                     YSYQYVDHPIFQTIVQDNYNVGGEIRYVNSHPIFGHENKFLIGNQPRYGDTNQRRFVN
                     NLGKRGIQTQNLTLETTNLSTYLEDQFNVTDDFLVIAGGRWEYSIRQGRNQLYSPFTG
                     DLNTSDTGVRHFNALIPKMGFVYQTTGTSQIYGNVSRGYEPPINVELTSSINPDGTVP
                     TTPFINLDAQRAWQFEIGTRGSTADNRYTWDLTFYNLEMQKEILTFNINNQPTYQNAK
                     GTRHTGIEAGGSMVVANGLLVQGPKNQSDTISVRTAYTWSLFKFTDDVYGGGDNGTQK
                     FLVAKDGNRVPGAPEHQLAGEVRYDHPAGFFIAPNFEWVLAGYYVNSENTAKNSAYFL
                     VNLKAGYNYSKNLSFFAEGRNLSDQTYAGAVVVNDSLGRYFNPGQGISGFGGIEWKF"
     gene            10093..10503
                     /locus_tag="PKM72_04150"
     CDS             10093..10503
                     /locus_tag="PKM72_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNP60008.1"
                     /translation="MRLPAATQKKARIEIIPMIDTMFFLLVFFMIATLSMTIQHGMPV
                     NLPTADSSTDKVPEQISLTLTHEGMLYYNKDRIELPELEVRLATLRQTDSDPSVVINA
                     DEQVSHGQVIKVMDIIRLAGISNMAIATKPNSES"
     gene            complement(10890..11117)
                     /locus_tag="PKM72_04155"
     CDS             complement(10890..11117)
                     /locus_tag="PKM72_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60009.1"
                     /translation="MGSPSAACMLCHAITVMCSCPVTAMKDPERNSSNRDSTWLLLER
                     NTAYQWRMEIIGPFCSRRNDALGMWVQGRIR"
     gene            11116..11322
                     /locus_tag="PKM72_04160"
     CDS             11116..11322
                     /locus_tag="PKM72_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNP60010.1"
                     /translation="MNKQLLRVGEAAQTLNVSRWTIYRWVEEDRLKATKIGKGSLRIF
                     RDSIDALIQQNRKNHWEFAITECQ"
     gene            complement(11404..11565)
                     /locus_tag="PKM72_04165"
     CDS             complement(11404..11565)
                     /locus_tag="PKM72_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60011.1"
                     /translation="MSLLIMVPVVDVEWSRVGKASGHAQEAHAEHESRKRLCESGLPH
                     LGPLHMQQF"
     gene            11904..12650
                     /locus_tag="PKM72_04170"
     CDS             11904..12650
                     /locus_tag="PKM72_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60012.1"
                     /translation="MYSYSFKCFRPTSSFTLLIVLFLFFPSLGFGREQAILSFQLDPR
                     PFSPALCQSSGHDRSIRIQVAKYHDLIIRKVPGQWIPAESREARIKQYVHFIKLQDLT
                     DGIVTQGAIAFDRNYAVIFTAEHPEGIARSKFMASLPASQQQLGNAALNTFLHLRQVL
                     YAAIDSCRSYPPFVVYGTDEEIDRIEAARRIFAKRVNQFNSDPTALSAFTFHETGMAD
                     GFVDPDAGMGTNVSYQAMNPLNFHVPSQYP"
     gene            12884..13435
                     /locus_tag="PKM72_04175"
     CDS             12884..13435
                     /locus_tag="PKM72_04175"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNP60013.1"
                     /translation="MQRMSIAFPAFVVLVCSFFTMPLAVQSGAMPKDGELAFPSDYTS
                     FPVFLSGLQKPDAVRDLYVNPIGARNQPGQAFTNGSILVMAIYNAKKNVEGTLEKGAD
                     GNLVKAELAKVFVMQKGAGWGKGAPENLKNGDWIYSAFNANGDRLEVDYTTCRGCHLP
                     LGESKDFVHRYDEYFEKRMHATH"
     gene            13938..14825
                     /locus_tag="PKM72_04180"
     CDS             13938..14825
                     /locus_tag="PKM72_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNP60014.1"
                     /translation="MAKSKRQKHSVDTEAPRKTSNTPASLHSGSKTLIDLLLIILAGA
                     GLMLTAYLTYTASFEVHPAFCSEGSGCDIVQSSRWATFLGIPMALWGFVTYMVIAGLA
                     WSGRGQSGSGSALIYVAMSGFAVSAYLTIVSVMEIEATCPYCLTSFAIITTILGLALI
                     QRPPNWTKSLKEAVMVGLLIVGGLHLHYGGWFDTAAGPEDPQLQALAIHLNETGAKFY
                     GAYWCPRCQEQKATFLSSAKRLPYVECSSGGRGSGLTAPCFKADIKTYPTWIIGERRL
                     TGLKTPQELARASGFDWQE"
     gene            complement(15200..15856)
                     /locus_tag="PKM72_04185"
     CDS             complement(15200..15856)
                     /locus_tag="PKM72_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60015.1"
                     /translation="MPCTQVGQWYRVGSGMCVVLAVGFLMQSVAMANPHEPREVGPYT
                     LAVGFRIEPAFEDAVNALDFFVTRTSDGKPISVSNGDVVDIEAQVQFRKEESMGSAII
                     QEDRLAKPEQAFGADNRYNAWFKPTIDGAYGFRVRGEINDLSDPQAGPLFFDEVWVCG
                     AGTQNPQGSRFGCVEDPQTFPKGVLDHRGIDHHGYQDDDRRKFFKEGGSRKHGSRDRD
                     "
     assembly_gap    16391..16490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16491..16686
                     /locus_tag="PKM72_04190"
     CDS             <16491..16686
                     /locus_tag="PKM72_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60016.1"
                     /translation="HNTISSPKSGQVDLYHARETNQLIYHDEMDEIARLPSVVLNVLR
                     IWPLTPHDSQHPTKDPRSSL"
     gene            16691..17059
                     /locus_tag="PKM72_04195"
     CDS             16691..17059
                     /locus_tag="PKM72_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60017.1"
                     /translation="MTRQPGQFSLWLICLLIGIRFVAAFGAYICVADWEIRTIRTVHI
                     HAAGSHSHCQHGQSTFNPFVGWACTVYQDDQMLLLPDKPYMPLQGSVLAPLVIQVISL
                     PDSPVIAAHGRSPPFSARIS"
     gene            17289..19622
                     /locus_tag="PKM72_04200"
     CDS             17289..19622
                     /locus_tag="PKM72_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNP60018.1"
                     /translation="MSSAGSSRVITRMPTCSRFLTRVAHIVATLFCLTGLTACMGPAK
                     PVPPTQSHAEQAPPATETVEGPKTLDPAPAPVPEPRIPLLPFPPIGSGQSIEEQDKVE
                     VPVVEAKPVHVTAQRESYNVERATSATKTDTPIMETPISIQVIPQAVLQDQQAIQIED
                     AVKNVSGVFPGFTFGGFGEEFIIRGFNTNFNNYRDGFRLSADRLPLANIERIEVVKGA
                     AANLYGRIEPGGMINLVTKRPQAESMYSLNQQFGSYNEYRTTADATGRLTTDGTLLYR
                     FNLEYLNKDSFRDYGFTKRIFVAPSLTWKMTPRTQLDLDFNYLNQDTREDYGIVAVGN
                     RPASIPRSRYLGEPTTDKSNTGFYNSTATLTHAIHENWKVRARFNYARRDVVDPQTSG
                     ASLDEQTGELQRFFYKGNATGNTYMGTVDLTGRFDTAGIEHDVLVGWDYYGGFTDVKS
                     TSLAANPINIYNPQYDPTNLAGVKNNFFIDQRTQWNGLYFQDQITLFDKLHILGGGRY
                     DWATQELGLAFGADQSVADATNNINKAKNNRFNPRAGIVYQPWQWLSLYGNYVQSLGS
                     ANLAFDASGNILDPQYAEQYEAGFKTAFFNDRLTSTVAFYHLTKENLPVQVPGQPFSE
                     AVGEARSQGVEVDIAGRITEGLSIITTYAYTDAEIMKGDNKGNRLWNVPKHAGSVWLK
                     YDMQQEPLRGLTVGGGVFAQGKRQGDNQNSYQLPSYARVDALMKYQIPITMARMTLQL
                     NVNNLLDQKYYGGTLGDRFSINVGAPRTFIGSIRIAY"
     gene            19573..20847
                     /locus_tag="PKM72_04205"
     CDS             19573..20847
                     /locus_tag="PKM72_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNP60019.1"
                     /translation="MSEPHAHSSGQSELRTKGEGRDRDARNRRRTFWLKIHRYLGLIP
                     GAVFAIIGLTGSILAFWPELEVWLHPGLWKVNPQEEQFEFRPLDEIVAAANTAIPSDG
                     TPYALVFPRMPDTAFMVSYSRPAPNPEQLEWHQVFIDPQTAQIKGQHLRLDLERPWRG
                     TFFDFVLRVHSTLALGARGSTVVGVLALLLLVSLLSGLILWWPSFGQLSGAFTIKRGA
                     GRIRRVYDLHKTLGLYTSMLLVVALSSGLYLVFPDFGKNLIGMFSAITLVPEDLASRP
                     LPGEQPIGFDRAAAIANGYYADGRFQWIFFPQGQEGFYRIVKKEPHERTDILPARALW
                     IDQYSGQILHTYDPEQFTAGDTFERWLFPLHSGEAFGLPGRIGIMILGLVPTILFVTG
                     LMRWKHKRKKRNLPKMFRPTTSRKGIDSQART"
     gene            21438..21752
                     /locus_tag="PKM72_04210"
     CDS             21438..21752
                     /locus_tag="PKM72_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60020.1"
                     /translation="MMKHMMAFILMVMLLMVPSFVFAHGNAIHIFGTVTESAQEQIVV
                     TTPKGESVTLIIQEKTIFQLDGITTNTARPAVGDRVVAEAEKEADDLVAHEIRFATPK
                     PK"
     gene            21925..22386
                     /locus_tag="PKM72_04215"
     CDS             21925..22386
                     /locus_tag="PKM72_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNP60021.1"
                     /translation="MVFTLLSPPVPRTFRIPYLFVLACLLCLPSHLQATESSAQPEVE
                     TFPELTVTDSRIHTDYSWLADSLGHRMAELNRYPHDARRNGWEGKVVLRVTIGAEGQL
                     RDVIVQDSSGYEPLDQAAQEAVRLACPLPLQHKIHRPEVAITLPVVYRLSH"
     assembly_gap    22397..22496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22717..24096
                     /locus_tag="PKM72_04220"
     CDS             22717..24096
                     /locus_tag="PKM72_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNP60022.1"
                     /translation="MKSKLTPAIYSRIWPDYQTVWRWHFYAGLFCVPFIVLLAISGGM
                     YLFTPQINAWLDGPYDRLFLTGPNATAQAQVEAALHAVPGSTLSAYELPKDQDDATRI
                     IVNNRGERLRVYVHPETLEILKTIPEEDRFTRMVFHFHGELLMGNIGSVCVELAASWT
                     IIMIVTGLYLWWPRGHEGIGGIVYPRLNRGSRGFWRDLHAVTGFWISLFVLFLLVTGL
                     PWAKVWGEYFKEIRRVTGTAVVEQDWTVGATSRNTPKDLSGEHLAHIGQTIPPANIVD
                     PPLDYSPIDILLPAVHALDLPPPVFIIPPSHIEGYWTAKSDTQNRPQRVKVMLDATTG
                     AVLQRKNFEDRHVLDRIIGFGVAAHEGQLFGWPNVVLGVFTTGGLLMVVTSGILMWWR
                     RRPSGLLGAPPLPQAQPFSCGFMIFLLFFCLYLPLFAASLTSILLIEKLLIRRVPPLA
                     LWLGLEKRV"
     gene            24318..25607
                     /locus_tag="PKM72_04225"
     CDS             24318..25607
                     /locus_tag="PKM72_04225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNP60023.1"
                     /translation="MIRPVFFGATSVVLSIGLGGVVSLALGAEPSVSMIQAGPKHIID
                     HKGMQTTGPMAQLDTDGTLHLAWGGEKQEERGVFYLKVPPQQEEFPEPTKVNPSNPPA
                     ASLHEPPALALGHNNDVYLTWTTPHPNAGGKPFANLLLLSRSVDGGKTFLPPVQVNDD
                     EAVTGHSFDNLTVSPNGSVHISWLDAREGKKDPATYTVVSQDQGHTLSPNLKIDESSC
                     VCCRTHMTTASDGTVYLAWRKVLPGAVRETVVSRSTDNGKSFSPPVIVGHDQWVFEGC
                     PHRPATIGVDQQGRLYVTWYTEGPDDTPGVYLAYSDDQGKTFTPRRLLNMSKGTFPDH
                     PQLAVNQAGHLLVSWEEQSPVRREVVFTYSLDRGQTFSSPNKVNEKKAKSPAVALNDA
                     GQAVIAWSEQVVFPAWSTVVQPLSFPTPQTAQLHPIP"
     gene            25655..26125
                     /locus_tag="PKM72_04230"
     CDS             25655..26125
                     /locus_tag="PKM72_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNP60024.1"
                     /translation="MGNPSSVPFTLSRVDLSQSLPAFQLRTLQGTPVHSNELAGRVLI
                     LNFWATWCGPCKEEMPSLERLRQKFPPDQLAILAVTTDIRPQEIKVFWQQLELHFDVL
                     LDEQEELSQALMVRNLPTTVIVDTHGHLSQRIMGPREWDSPESVAHIHNLLNPK"
BASE COUNT         6448 a         7261 c         6010 g         6234 t
ORIGIN      
        1 aaataacctg ttgtttgaag agcgcttcag cattgcctcc caataaaata tcggtaccgc
       61 ggccggccat gttcgtggcg atggtcaccg aacccttttg cccggcctgg gcaataattt
      121 ctgcttcttt ttcatgaaac ttggcattca agacatggtg ttttacgcct cgatgcttca
      181 ataaggctgc aagacgttca gatttttcga tagaaatcgt gccaataaga accggttgtc
      241 ccttctcatg atattccttg atctcgtctg caatggcctc gaatttctcc ttttcggtcc
      301 gatagaccac atccggatta tcgattcgaa tcattttgct attggtgggc accacattca
      361 cttccaggtt gtaaattttt gcgaattccg cagcttcggt gtccgcggtt ccggtcatgc
      421 ccgccagttt gttatacata cgaaaataat tctggaacgt cacggatgcc agagtttgat
      481 tttcgttggc aatcttcacc ccttctttgg cctccacagc ctgatggagt ccatcactcc
      541 atcgccgtcc cggcattaat cgaccggtaa actcatcaac aatcatcacc tcgccatctt
      601 tcaccacata ctccacatcc cgatgataga gcacatgggc ttggagagct ttaataacgt
      661 gatgcaccaa actcaggtgc ttcaagtcat agagattatc gactcccaac agtttctcaa
      721 catgcgcatt gccttcatcg gtcagagata ccgtttttgt tttctcttca atcgtataat
      781 cctcttcccg tttgaggtga gggatgatcc gatctacctg gaaataaagg tctgtgcttt
      841 gttcggcggg tccggaaata atcagcggcg tccgagcttc gtcgatgaga atgctgtcaa
      901 cttcatcgac aatggcaaaa ttcaattccc gctgaacccc ctggctcaag tccgtgacca
      961 cgagattatc ccttaaataa tcgaacccaa attcattatt ggtcccgtag gtaatatcgg
     1021 ccccataagc ctcacgtcga gaacatggcc gaagaaattg caaccgttta tccggggcat
     1081 cataggttgg atcaaataaa aaggaggcgt catgttgaat gatcccgacg gataagccta
     1141 aaccatggta gagcggtccc atccactggg catcgcgctt tgccagataa tcgttcactg
     1201 tcaccaaatg aacccctttt ccctccaacg cattcaaata gaccgggaga gtggcgacaa
     1261 gagtttttcc ttcacctgtg cgcatttcgg caatacgtcc ctggtgcaag accattcccc
     1321 ctaacaactg tacatcaaaa tggcgcatgc caagctggcg cttggacatt tcacggcata
     1381 cggcaaacgc ctcagggagc aaatcatcca aagactcgcc agcttctagt ctttttttaa
     1441 atattgcggt ttttgccgct aatccctcgt catcaagggg ttgcaaggaa gcctcaaatt
     1501 cattggctcg aatcacttgt ggtctgagcc gcttgagctc ccgatcattt ttgctaccaa
     1561 agagaatatt taatagacga acaaacatat cgaccttacg aaatagggtg ggagaaaatc
     1621 acaatggata gggaatgagg gctagacaca cctttcaaac catacagttg ccctagtata
     1681 ccgaattacc ggtgggaatc aactcacagg aaaaggattc atgatcgcaa agatccatcc
     1741 tgtgacagac gacgacacga aacgaaggac aggcatttgc ttgacagaat ctttcgggtt
     1801 tccctagcat aagcctcctt tattattcag ggttttgaac atttctggtc tgtagataac
     1861 atgccatatc gaaaaagcct caccgttgta ctcattctga tgctcttcct catcatgggg
     1921 agcatgcatc ggacctacgc ttcgcacatt acggaacata cccatcacca cacatcagcc
     1981 atgcatagca cggcaatctg ttcctggatg tgcgcggcgg cacaaacaat ttccggggat
     2041 aaccaggaaa tttcccctag cttccaactc cttgccatta tggaatatcc gattcctaaa
     2101 gtccctactc tcatttccac tcattttctt ccaaccagag ctcctcctcg ataaatcatc
     2161 cgaagcctgg ttcccaaacc gctttcacca taacgagttg agttcgggaa cggcctcaca
     2221 acgcatggtc agaggagggg acggccgaca tcttcctgac aggagaaacc gaacaaggga
     2281 caacctcttt aagggggtag tgagtttctt aacgtcaaag atgccagctg gcttgatttg
     2341 acgttatccg caacctagaa aaacaaccag tggatataat tcaacacgac cggtcgtcat
     2401 atttcgtaat gggaaaaggt cttttggtac atcaacacaa tctgatgttt ttcattgttt
     2461 tatctctatg gtgcactttg gcctccaacc aaacttcttt ggcggaaaac ctcggtgaga
     2521 gcacagagcc catagaaacc ttagacgtca ttgaagtgac tgggacaacg gtgacaaaaa
     2581 ccagtagaac attttcattt ccgttgccca cgattgaccg cccatggccg ccaacgacat
     2641 ttcataccct tggggctccc gatcttgata tggataatct gccacagccc gccatacccc
     2701 gaatactcct agaccggatc ggaacaacac gcgggcgatt gaaaagcgtc aaacccctga
     2761 aaaccgaacg ccccctctat cccagaatgg ccagagaaca gggatggcat gggaaggtgg
     2821 tcctgcggac gcacataagt tccgatggca cggtcacaag tgccacagta aaggaaagtt
     2881 cgggattttc tctcctggac gaaagtgccg ttcaatcggt aaagggctgg tcctttgaac
     2941 cagcaaaaaa tggagaattt accgttgcgt ccaccgtgga tttgcccatt cgatttgatt
     3001 tgcttcaata aacctgggga atattgccca tatcaataag gttagcccat gatacctcct
     3061 ttctcgaaaa tcagactcat cgcgtttttc cttctcgtca cccatatcgg cctgggcagc
     3121 ccctcaagtc aggctggaga agcacaggac cttctttccc aagccttttc atcctatgaa
     3181 caacaacaat ggacttcggc cattgcctcc ttagagcgtg cgctggcctt gtacccggga
     3241 tacgctgagg cgcaccatct gttggggtta atcttcaaca atcttgaaaa accggataaa
     3301 gccatcatgg agttacaacg ggcggtggag gcctatccca aatttgctca agcctatgtg
     3361 gatcttggat ccatctacca gcatcaggcc caatttccaa aagccgagca atcattcaat
     3421 cttgccttga aggcctatcc caagtttacg gaagcccgca ttgcgttggc ggcgttgttt
     3481 gatcagcaac aacaagcgca gaaagccatt aaggcccatc aggccgtctt ggaactcgag
     3541 cctcatcgcg tcgattcgtt atacggcatc gcattctggc tggcccagga acaaaagcct
     3601 gatgaagccc aacgttacgt cgaccagctg gtctccctcc atcccacgta tgtcaatgga
     3661 tggttaatgg ccggatcact cgctcaacaa tctgatcacg tggacaacgc cattcaggcc
     3721 taccaacatg ccgtaaaatt aaacaatgat ttagccagcg cctatttcaa tttaggcatc
     3781 ctgtaccggc aaaaggaaga tttcaaggaa gcggcacaag catttgaaca cgtcaccgct
     3841 ctcgataaaa acaatgcgga agctcatgtc aatctgggag tcgtgtatgc ggccctgtcc
     3901 caaattgatc aagctgaaga agaatatcaa acagccttat ctttgaatga tcaattggcc
     3961 gaagcccact acaatcttgg catgttgtat gaatttcatc gcaatgcccc ccaaaaagct
     4021 ctcaagcact acgaagaatt cttgaagctt gggggccagg acgaacgaat tcaaacatta
     4081 ttacaacgga gcaaatcgtg aactccacgt ttgaagattc ctcttccaat ttcaataatc
     4141 gaatttttct cctgagcgcc ttgggaagcg ttattcttca tgcaggaatt cttgccgccc
     4201 tgacgtatta tccaagtccc cctgaagtta aagacctcac cccaacagtc caggtgacat
     4261 tggttccggc tctccaggaa atcagtccca ccccacaacc gcccacgaaa cctctgacac
     4321 caccccaacc ggattttgca caacccacgc caccgtctcc ccggacccaa cctacgccag
     4381 ccatgccggc gacaccgatg caggcatcaa ttaaaccgac accttccaaa cccctggctc
     4441 cgccgcctct tcctcaggca aagcccatgc tcaaagacac acgaacggtg caggccatac
     4501 agagtcgtca gatgatgaaa atggccgttc ctcctcaaca ccggcaaaga cctcagcttt
     4561 caccggcgac accgcagcgg gaacaacccc ctgccaggac acctatgcac atgaacaatc
     4621 gtcaaccaat agcgcagcac acactgcctc ccccgccaaa cccgtcggct cgtccggcat
     4681 taaaaactcc gccggcgatg gcagccggat cctcaacgtc caagccgaga attgtcgcct
     4741 cgaccaaacc actctatcct cgtgtggcgc gtgagtctgg gtgggagggg acggtgattg
     4801 tgcgaacatt gattaacacc gatggcgctc ccagccaggt ggaaattcga aagagctgcg
     4861 gtcatgaggc gttagatctg gctgcccagg aggccattaa aaactggaaa tttcagccgg
     4921 caaaagacgg gaacatcccc atagccaaat gggtcgacat tcccattaaa tttgacctga
     4981 acagttaagc gacgcgagaa gaagggagta gaggcatgat tcaattactg atgggtggcg
     5041 gatggatgat gatccccatc ctgggctgct cattattagc tctgaccgtc agcatcgaac
     5101 gtggattcca attccgatcg ttgcgcttca gccgcctggc ggaccggatg atcaaactgg
     5161 ttgaacaggg aaaatggacg gatgcgctgg cattagctga acaacgccag agccccacac
     5221 tccgcgtgct tgcggcaggc atatttcatc gagaccaaca accggacaaa gccatggaag
     5281 cagctggaat cggcgagctg gcccgattaa agcggggatt atccgtcctg gatacgatca
     5341 ttacgttggg tcccttactg ggactgctag gaaccattat tggaatgatc gattcctttg
     5401 acatcatgtc cgaatcagga ctaggaaatc cccacgcggt cacaggtggt gtggctgaag
     5461 cactcatttg caccgctgca ggaattgggg ttgccgtcat tacgctcatt ccctacaact
     5521 ttttcctttc cctagtcgaa cgggaaaccg agcgcattga acaatatgcc accaaacttc
     5581 agtcgttcct gggacattcc taattcccac attcagctta cgaacggcat ggacaaccca
     5641 gagcaacaaa gcagagaaat cgtagccaaa ctcagaaggt tcattcattt agcatgcttg
     5701 aagcctccag gcatgattat tcagggcgaa agaaaatgga ttctgagtca acaggcacca
     5761 tacaccctgt tgcctttgac catcctgtta ctcattagtg gactgggatt gatgggatgt
     5821 agcattccca ctcattcccc accaaccacc aatatcgaat ctttcgacaa ccggaagact
     5881 gccaaaacct atgaatttat caatggccaa tggtttgatg gacaaggctt caccagggat
     5941 acctggtatg cggtcgatgg ctcacttacc cgcctccggc ccaatgttgt tgaacagacc
     6001 attgacctga aaggcgggtt tgtcgtgcca ccgtttggcg aagcccacac ccacaacgtc
     6061 gaaggtgcct ggaacatcga tcaggtcatc aatcattatg tccgggatgg tattttttat
     6121 gtaaaaaata ccaccgacat tcccgagttt gttgagcaaa tcgacggaaa actcaacaca
     6181 ccggagacca ttgatgcgat ctttgcccat gccggtctga ccgggaaaag cggtcatccc
     6241 attgctctat acgaagacat acttcgcacc catcgttatg aaccggtcat tggcaaacgt
     6301 aaaaaaggat ggttcaacgg gcgagcttac tttgctcttt cctctctgaa ggatgtggaa
     6361 acctactggc cggccatcat ggcaaccaag ccggatttca ttaagctcta tcttgccgac
     6421 tcgaaacatt ttgaaaacgg cacccgctcc accactcatg ggtttcggaa cggcctgaat
     6481 ccgcagctta tcgagccgat tgtcacgcgt gcgcaccagc aaaacctgcg tgtttccgca
     6541 catattgaaa cagttgatga ctttcgaacg gccatcaaga atcatgtgga tgagatcgcc
     6601 catctcccag ggtggttcct tccttcgccc gaccaagagt cagccgtgcg attaacccaa
     6661 gaagatgccc aactggcggc cacacaccgg accgtgattg tcacgacgac cgtcgccgag
     6721 cactttcacc cggcaggaca tcataccaag acggacgcgc atccccactc tcctgcaaat
     6781 tcccaagagc accaaccgcc acccgtgaaa aatgtccttg aggtcgccaa gagaattcag
     6841 atggaaaacc tcaagttact gcatcaggcc ggggtcacca ttgccatcgg aagcgaccat
     6901 gcggaaactt ccctggcgga agcacttcac cttcatcaca tgggaatttt tgacaatctg
     6961 accttactga aaatgtggag tgagaccact cctcaaacta tttttccttc aaggaatatt
     7021 ggacgattgg acgagggata tgaagccagt tttcttgttt tacagggcaa tccgcttgaa
     7081 gacttcgaac aggttcaaaa cattatcctt cgtatgaagc aagggtcacc tctgtcatgg
     7141 agcgaaactc accatacaga tgccacagcc aaacatgcgc attaatcttc gagtcgatct
     7201 acaatgggca ggatttttaa accacaagat ataaacgctt caacatgcct ggcaagccat
     7261 cgagccctcc catgatgccg cgccgcctta cactcccttc aatcatcttc gcggtggcgt
     7321 atttcgctct ggcgagcata gccacgtatt gcgtggcatc tcatgagatc caacattctc
     7381 caatcaatca tcattccaaa aacaccactt cccattcctc aatctgctca tgggcctgtc
     7441 aggtcagttc gaaagcgaac tcggccgaca cgacccacaa cgcacttcct acccacaccc
     7501 tattaatcgt tggattcgtt ttcttttttt ccgtcatccc tgcacaggca acgctgagtc
     7561 ggactctcgc tcgcggccct ccggtttaat caggttccac gattccaact taatattttt
     7621 tgcagccata agggcagaac cctgttcagc ctgacctgaa tcccttcatg cgtgcaggaa
     7681 aggatacgtg aaatccatga tcgacctaaa gtacttgaaa atcaaaaaca tgcaactagc
     7741 cttgcagttt tgcgcggcaa ttagctttct cctaatcgag ccctccctgg gtatagctga
     7801 agaaataatt gaaacacttc caactgtgac agtagaagcc aaacgaatcg aaaacatcga
     7861 tgtagtcaag gaagaactcg ctcgccggcc cagcagcacc atcctgatcg aggaaaaaga
     7921 aatcgaggaa tcccgtgcgt tcaatttaac cgacgtcctt caattcgctc caggcgtgcg
     7981 attccaatcc cgattcggtg ccgacgaagg acaattccaa atccggggga cttccctcag
     8041 gaacaacttt catcatcgtg gtattaatat tctcatcaac gggatctatt tcggcgatgc
     8101 ggacggattc tccgattttg aatccatcga cttgatggcc tatgagcgta tcgaggtgta
     8161 taaagggtcc aacgccctcc ggtatggagc caataccctg ggaggagcca tcaattttgt
     8221 gccccgcacg ggctacaacg cctccctcta ccaagcgcgt ggattggctg ggagtttcgg
     8281 actctattca ggacaggtct ccagcggcca ggtcaccaaa ccctttaagg tcggcaccat
     8341 ggacaccacc gccgattact atatcagcgc ttcgggcaat catcaggacg gctttcagga
     8401 taacagccag caggcacgcg gacggttaaa tgccaacttc ggatttcaga tcggcgatca
     8461 ccaggaagtg cgcgcctata tcctgggagc cgaggtgtca gaagcactcc cagggtcgat
     8521 tacccaagcg caattggatt ccgatcgaag acaggctaac accttggggg gaatcaatcc
     8581 gttttcctgc gggccaaacc aggcctgcaa atggggacgg tactatagtt atatccgggt
     8641 cggggccgcc tatcgcaatg aattcgcagc ccaccagttt tttgagatta tcccctatta
     8701 ttcctaccaa tacgtagatc atcctatttt ccaaacgatc gtccaggata actataatgt
     8761 gggaggagaa attcgatacg tcaattcaca tcctattttc ggacatgaga acaagttcct
     8821 catcggcaat cagccacgat acggagacac caatcaacgg cgttttgtca acaatttggg
     8881 caaacgcgga atacagacgc aaaacctcac gttggagacg accaatctca gcacctacct
     8941 ggaagaccaa ttcaacgtaa ccgatgattt ccttgtcatt gcaggcggac gctgggaata
     9001 cagcatccgg cagggcagga accaactcta cagtcccttt acgggagatt taaataccag
     9061 cgataccggc gtacgtcatt tcaatgctct tatcccaaag atggggttcg tgtaccaaac
     9121 caccgggacc tcgcaaattt acggaaacgt cagccgtggg tacgaacccc ccatcaatgt
     9181 cgaattgacc tcatcgatca acccggacgg cacggtcccc accaccccct tcatcaacct
     9241 ggatgcgcaa cgggcctggc agtttgaaat tggcacacga ggctccacag cggataaccg
     9301 ctatacctgg gacctcacct tctataacct ggaaatgcaa aaggaaatcc tgacgtttaa
     9361 tatcaataac caaccgacct atcaaaatgc caaagggacg cgacacacag gcattgaagc
     9421 aggaggcagt atggtggtgg ccaacggatt gctggttcag ggccctaaaa accaaagcga
     9481 cacgatctcc gttcgtaccg cctatacctg gtccttattt aaattcaccg atgatgtgta
     9541 cggcggagga gacaatggaa ctcaaaaatt tttggtggcc aaggacggca accgggttcc
     9601 cggggcgccc gagcaccagc ttgccgggga ggtacgctat gaccatccgg ccggattttt
     9661 tatcgccccg aatttcgaat gggtcctcgc cggatattac gtaaacagcg aaaataccgc
     9721 caagaactcg gcctatttcc tcgtcaatct gaaggcgggc tataattaca gtaagaacct
     9781 gtcctttttc gcagaaggac gaaatctgtc cgatcaaact tatgcgggtg ctgtcgtggt
     9841 caatgacagt ctgggccgat acttcaatcc cggtcagggg atcagcgggt ttggtggaat
     9901 cgaatggaag ttttaatgtg ttcgggccgg acacaatcgt tcagggaacg gaacattgag
     9961 gttgtcacac ctagttcgtt attctaggtg tgacacccaa tggcttatca actgaagacg
    10021 gatattttaa agatttccat tcacattatc tgttaacctt ttcgcaacga caaaccgggc
    10081 attacgattt ccatgagact acctgccgcc acacagaaaa aggcccgcat cgagatcatt
    10141 ccgatgatcg acacgatgtt ctttctcctg gtgttcttta tgatcgccac gctgtccatg
    10201 accattcaac atgggatgcc ggtcaatttg ccgacggccg actcctcaac ggataaagtt
    10261 cctgagcaaa tctctctcac gctgacccat gaaggaatgc tgtactacaa taaggaccgc
    10321 attgaactac cggaactgga agtccgcctg gcaacccttc ggcaaaccga ctcggaccct
    10381 tcagtcgtca ttaacgccga tgaacaggtt tcccatggcc aggtcattaa agtgatggac
    10441 atcattcgat tggccggcat ttccaacatg gcgattgcca caaaacccaa ttcggaatca
    10501 taatcccccg gaagccatga actacacccc agcaacccca ttcaccaatg tagggactcg
    10561 attggcacat gtccttatcg ggctactcat tgggttacac gtcgtcctcc tgggtgcggg
    10621 agcgttctgt gcttcaagat cccttgatcc caacacctct caccattccc acccggccca
    10681 atccctctct cttctttgct cgtgggcctg tcatgtcgcc cagatcgcct caggtgccag
    10741 tccgtttgct caatggtcgg cgctgttttt tcccctcatg ttctggtccc tgacacccgt
    10801 attcatcttt tcccgaacct cgcccttaca cagattctct cctcgcggcc cacccgttcg
    10861 ttttttggtt catggataga ctcggatttt caacgaatcc gtccctgaac ccacatccca
    10921 agcgcatcgt ttcgtcgtga gcagaatggt ccaatgatct ccattctcca ttgataggcc
    10981 gtattgcgct caagaagtaa ccatgttgaa tcccggttgg aactatttcg ttccggatct
    11041 ttcatagcag tgaccggaca cgaacacatg actgtaatcg catgacacag catacaggct
    11101 gcggaaggag aacccatgaa caaacaactt ttacgtgtcg gtgaagcggc tcaaaccttg
    11161 aatgtcagcc gatggaccat ctatcggtgg gtcgaagaag atcgtctgaa agcgacgaaa
    11221 attggaaaag gcagcttacg aatatttcgg gattccatcg atgcgctgat tcaacaaaat
    11281 cgcaaaaacc attgggaatt tgccatcacg gaatgtcaat gagcaacgaa taaactgttg
    11341 catcgatgag cacccgtcca tagacacctt gcaaagaaac agctagcttg acgggggaga
    11401 tcatcaaaat tgctgcatgt gcaatggccc aagatgagga agaccactct cacacaatcg
    11461 tttgcggctc tcatgctcag catgtgcttc ctgtgcgtga ccggaggcct tgcctacacg
    11521 gctccactcc acatccacca cgggcaccat gatcagcaga ctcatgggaa cacctggtgt
    11581 agctggacgt gccaagccgg gcaaggcctt cagacgctgt cagctgacat cctcaggtcg
    11641 ttttctctat tgggcattct tcccctcttc aaacctacag caccggatct cttccgacat
    11701 caccacagtg cttctcgtgc cccgcctcac ttttccctct tctaagcttc tcattggatc
    11761 ccgttggttt ttctcgctca tgaagatgaa agacgtgggt tctcagaaga gctgaaccaa
    11821 acctcagagt ccggagcact caccggctct caagcaggtt ttcaatttat tccttgatac
    11881 ctactcaatg gaggcactgt ctcatgtaca gttattcctt taaatgtttc cgaccaacca
    11941 gcagtttcac cctattgatc gtcctctttt tgttctttcc aagcctgggg tttggccgtg
    12001 agcaagccat tctttcgttt caacttgacc cacgcccttt cagccctgcc ctctgccaaa
    12061 gcagcggcca tgaccgatcc atcaggattc aggtggcaaa atatcatgat ctcatcattc
    12121 gcaaggttcc cggacaatgg atacctgccg aatctcgcga agccagaatc aagcagtatg
    12181 tccatttcat caaattgcag gatttaaccg atggaatcgt cacccaaggc gcgattgcct
    12241 ttgatcggaa ttatgccgtg atctttacgg cagagcaccc cgaaggcata gcgcgaagca
    12301 aatttatggc ctcactccct gcatcacaac aacaattagg aaacgccgcc ctgaatacgt
    12361 ttttgcatct ccgtcaggtc ctctacgccg caatcgattc ctgccgttcg tatccaccat
    12421 ttgtcgtata cggaacggac gaagaaattg accggattga agcggccagg cggatattcg
    12481 ccaaacgggt caatcaattc aatagtgatc cgaccgcctt gtctgcattc acctttcacg
    12541 aaaccgggat ggccgatgga tttgtcgatc ccgatgcggg aatgggcacg aacgtcagct
    12601 accaggcgat gaatccatta aattttcatg tgccttcgca atatccctaa cccgagaagt
    12661 tctggaaatg agagatcgat ataacacaga gaccgtctcg atcacccact cggctataga
    12721 tcccaaacca tcccgctgta gcagaagaca gtatccgtct gtagacggac tattgggtcc
    12781 atcgtatgct ggagccactg tctagacgtt tccaattttg gcttttcacg atctgccgat
    12841 tgcgtcaacc ggacgagtgt tcggcgaagg aggattaaga aacatgcaaa gaatgagtat
    12901 cgcgtttccg gcctttgtgg ttctagtctg ttcttttttc accatgcctc ttgcggttca
    12961 atctggcgcc atgccgaaag acggtgagct ggcatttcct tcagactata catccttccc
    13021 cgtcttttta tcagggctcc aaaagcccga tgcggtccgt gacctgtacg tcaacccgat
    13081 tggtgccaga aaccaaccag gtcaagcctt caccaacgga tccatattgg tgatggcaat
    13141 ctacaacgcc aagaaaaatg tcgaagggac tttggaaaaa ggagcagacg gaaatttggt
    13201 gaaagccgaa ttggccaagg tttttgtcat gcaaaaagga gcaggatggg gaaaaggcgc
    13261 tccggagaat ctcaaaaacg gcgactggat ttactcagcg tttaatgcca atggcgatcg
    13321 cttggaggtc gattatacga cctgccgggg gtgccattta ccccttggtg aatccaaaga
    13381 ttttgtgcac cggtatgatg aatattttga gaaacgaatg catgcgactc attaaccaaa
    13441 acaatgcatc ctttcctggt tccaggaaaa acagaacagc gtagaacatc cagggacgga
    13501 ccggcctcca accttggacg agtagggtga aggtggattg cactcctgca gcctcctaga
    13561 ccttcaccct gctcctatgc gcacccagcc tcttccctcc tcaacaccgc tcatcagtgg
    13621 ccaaaaatat tcaggctaag aggtccctac tccggcatcc aatcactcag aaccaatgac
    13681 cgaaagacta tcaccctatc caatcctgaa acaaaacggc agacaagggc ccgaccctat
    13741 ccctttctca agcaagacct tgagggacca aaagccgttt gaaatttcga tcccgtgctc
    13801 agcaattcat cattgggaga tacgcgcctg acatgacctc atcaaaagtc tcacccacgg
    13861 tcgggagttt ctgaaacatt gattgtcggg atttcattgg caggaatccc atgatacact
    13921 ctttcacgag tcgcgctatg gcaaaatcaa aaagacaaaa acactcagtc gacaccgaag
    13981 ctccaagaaa gacatcgaat actccggctt ccctgcactc aggttcgaaa accctaatcg
    14041 accttctcct catcatcctg gccggtgccg gactgatgtt aaccgcctac ctgacctata
    14101 cggcatcatt cgaggttcac ccggcatttt gcagcgaagg ttcaggctgt gacattgtgc
    14161 agagcagccg atgggctaca tttctgggaa tccccatggc cttgtggggt ttcgtgacct
    14221 atatggtgat cgcggggttg gcatggtccg gccgcggcca gtccggtagc ggatcggcgt
    14281 tgatttacgt ggccatgagt ggattcgcgg ttagcgccta cctgaccatc gtctcggtca
    14341 tggagatcga agccacctgt ccctattgcc tgacttcatt tgccatcatc acgaccatac
    14401 tgggtctcgc tctcattcaa cgtcctccca attggacgaa gtcgttgaaa gaggcagtca
    14461 tggtcggact actcatcgtt ggcgggctcc acctgcatta tgggggttgg tttgacactg
    14521 cggctggccc tgaagatccg caactgcagg cactcgccat ccatctcaat gaaaccggcg
    14581 ccaagtttta tggcgcctat tggtgccctc gttgccagga acagaaagcc acatttctct
    14641 catccgccaa acggctcccc tatgtggaat gcagttcggg cggaagagga agcggtctca
    14701 ccgccccctg cttcaaggcc gacatcaaaa cataccccac ctggattatt ggagaacgac
    14761 ggttaaccgg actcaagacc ccacaggaat tggcacgagc ctccggattc gattggcagg
    14821 agtaaacgat ccggcattgt cttcccctcc atctcattag acctccgttg ccgaccttcc
    14881 ctgtaccccc acattctcga ccccggatag tagaactatt tactcacgac acaggatcat
    14941 tatcttcatc ttcaaacccg tgtcccaccc atcggggtgg cccaggttgc gattacccgt
    15001 gcccaccgcc gaaacacgga aatcgggatt atcttctatc agaatggaat cgtcacggaa
    15061 attcaagaaa aaatacaata gagagggcca tcatcgcttc tcccggcaac accaccgatc
    15121 aaatccacga tgagggagaa cacgcatgcg cgaattcccc ccctcgctga agtcaccgat
    15181 gaaatgccaa tgatgtctct cagtctcgat cgcgggatcc gtgtttacgc gatccccctt
    15241 ccttgaaaaa ttttcttcta tcatcgtcct gatacccatg gtggtctatg cctcggtgat
    15301 caagaacacc tttggggaat gtctgcggat cttccacaca gccaaaccgg ctgccttggg
    15361 gattttgggt tccggccccg cagacccaca cttcatcgaa aaatagcggc ccggcttgcg
    15421 ggtcgctgag atcattgatc tcccccctga ctctgaaccc ataggccccg tcaatcgtgg
    15481 gtttgaacca ggcattgtac cgattgtcgg ctccgaaggc ctgctcgggt ttggcaagtc
    15541 gatcctcctg aatgatcgca gaacccatac tttcttcctt tcggaactga acctgcgctt
    15601 caatatccac cacatctccg ttactcacgc tgataggctt cccgtctgat gttcgggtca
    15661 caaaaaaatc aagagcatta acggcatcct caaatgccgg ttcgatccta aacccgacgg
    15721 ctaaggtata gggcccgact tcccttggct catggggatt ggccatggcg accgattgca
    15781 tcaagaaccc cactgccaac acgacacaca ttcctgagcc cacccgatac cattgaccaa
    15841 cttgcgtgca cggcatacca gaatcctcct ctttggtgaa tgtgaacatg gatgcgaaaa
    15901 ttttcgaaca accggaagaa atatatcctg tgatggaaga tctcgctacc aaaccacgcc
    15961 ttcattaacc aatcgttgca acatattttt tcacttcatg atttttcgaa tgtcaggaaa
    16021 agataccgac atggtctcaa gaaagcttga gaaggagcat gatatggaac cgcggttttt
    16081 ccgaacgggt tcgtaaggga gaactatcaa aataacaata gttacatgaa catatttgcc
    16141 agaaatattc cctgatgtag caccgatcag tagagatcgg tagaacaatc tcaccggata
    16201 ggatacattt tcccttgaag ctgagagatc actgcactct tccagcatca atcggagcgg
    16261 cgtgactcat ccctgaccca ccccgctccc tgtcagggtg aaggcattgg gacttgcaac
    16321 ctccttggcc ttcaccctgt tgttttattc tcatttgtcc cgcttttcca ggacaattca
    16381 ttggcggatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acacaacaca
    16501 atctcttcac caaagagcgg ccaagtagat ctgtatcatg ccagggaaac gaatcagctt
    16561 atttatcatg atgaaatgga tgaaattgca cgacttccct ctgttgttct caatgtactg
    16621 agaatatggc ctctcacccc gcatgatagt caacacccaa caaaagaccc ccgctcttcc
    16681 ctgtaggcct gtgactcgac agcctggaca gttcagccta tggctgatct gtctgctgat
    16741 cggcatccgg tttgtggccg ccttcggggc ctatatctgc gtcgcagatt gggagatcag
    16801 gacaatccgc actgtgcata tccatgcggc aggctcccat agccattgtc agcatggtca
    16861 atccactttc aacccattcg tcggatgggc ctgtacggtg tatcaggacg atcagatgct
    16921 tctcctgccg gacaagcctt acatgccgct gcaggggtcc gtactcgctc cactggtcat
    16981 tcaagttatt tcgttgccag acagtccggt gattgccgca catggccgca gtcctccctt
    17041 cagcgcacgc atctcctaat tacaagcacc acacaacatt cgattaatca tgagccgcag
    17101 ccggcctggt ccgtttcgac gcgtgtgccc tgggactgac atctcgcgat gtgagcagga
    17161 aggggtctct cctcacactg tttcctccca ccacgggcat gcggagtctc gctcccggaa
    17221 ccgaaccgtc tgcatcacac agtactgacg tccctgcatc cagggtgctc aggtaggagc
    17281 gtattactgt gagttcagcc ggatcctctc gagtcatcac tcgaatgccc acatgttccc
    17341 gtttcctcac gagggtcgcg catattgtcg cgacgctctt ctgcctcacc gggttgactg
    17401 cctgcatggg accggccaag ccggtgccac ccacacaaag tcatgcggaa caggcacctc
    17461 cagcaaccga aacggtcgag ggacccaaga cacttgaccc ggctccggct cccgtccccg
    17521 aaccacgcat ccctctttta ccattccccc caatcggctc gggccaatcg atcgaagagc
    17581 aggacaaggt agaggtgccg gtggtggaag ccaaaccggt ccatgtcacg gcgcaacgcg
    17641 agtcctataa cgtggagcgt gcgacgagcg cgaccaaaac cgatacgccg atcatggaga
    17701 cgcccatttc tatccaagtg atcccgcaag cggtgcttca ggatcaacag gccattcaaa
    17761 tcgaggatgc cgtcaaaaat gtcagcgggg tattccccgg atttacgttc ggtggttttg
    17821 gcgaagaatt tatcattcgc ggattcaata ccaatttcaa caactatcga gacgggtttc
    17881 gattatcggc tgatcgactc ccgctggcca atatcgaacg catcgaagtt gtcaaaggcg
    17941 ccgcagcaaa tctttatggc cggatagaac ccggcggcat gattaatctg gtcaccaaac
    18001 ggcctcaagc cgaatccatg tattcgttga atcagcaatt cggctcgtac aacgaatatc
    18061 gaaccaccgc cgacgccacc ggccgactga cgaccgatgg gacattgctc tatcgattca
    18121 acctggagta cctgaataaa gattcctttc gcgattacgg ctttaccaag cggatctttg
    18181 tcgcgccatc gctgacctgg aaaatgactc cacgcaccca attggatcta gattttaatt
    18241 atctcaacca agatacccgt gaagattacg ggatcgtggc cgtgggcaac cgccccgcct
    18301 cgattcccag atcacggtat cttggcgaac cgacgaccga taagagcaac accgggttct
    18361 ataactcgac agcgacgttg actcatgcca ttcacgagaa ttggaaagtc cgtgcccggt
    18421 ttaactacgc tcgcagagac gttgtggacc cacagacatc gggagcaagc ctggatgagc
    18481 agacgggcga attacaacga ttcttttata agggcaatgc gaccggaaac acctatatgg
    18541 gtaccgttga tctcaccggg cgatttgata cggccggcat cgagcatgat gttttggtgg
    18601 gatgggatta ctacgggggg tttacggacg tgaagtccac atccctggcc gccaacccca
    18661 tcaatattta caatccacaa tacgatccga ccaatttagc cggagtcaaa aacaattttt
    18721 tcatcgacca acggacacaa tggaatggtc tgtattttca ggaccagatc acgctgttcg
    18781 ataaactgca tattctgggt ggcgggcgct atgattgggc cacgcaagaa ttgggattgg
    18841 ccttcggtgc cgatcaatcc gtggccgatg cgacaaacaa cattaacaaa gccaaaaaca
    18901 atcgatttaa ccctcgcgcc ggaatcgttt atcaaccgtg gcagtggttg tcgctgtatg
    18961 gcaattacgt ccaatccctg ggtagtgcga atttggcgtt cgatgcgtcg ggcaacatcc
    19021 tcgacccgca atatgccgaa cagtatgagg cgggattcaa aacagcattt ttcaacgatc
    19081 ggttgaccag cactgtggcg ttttaccatc tgaccaagga gaatctgccg gtccaggtac
    19141 ccggccaacc gttttccgag gctgtgggag aagcgcgcag tcaaggggtt gaagtggaca
    19201 tcgccggaag aattaccgag gggctgagta tcatcaccac ctatgcatac accgatgccg
    19261 agatcatgaa aggggacaac aaggggaacc ggctctggaa tgtgcccaag catgccggga
    19321 gtgtctggct taaatacgat atgcagcagg agcctctgcg tggattaaca gtgggtggcg
    19381 gcgtttttgc ccagggtaag cgacaaggag acaatcagaa tagttatcaa ctacccagct
    19441 atgcccgagt cgacgcgtta atgaaatacc agatcccgat tacgatggcc cggatgaccc
    19501 ttcagttgaa tgtcaataat ctcctagatc aaaagtatta cgggggaacc ttgggggatc
    19561 gtttttccat taatgtcgga gccccacgca cattcatcgg gtcaatcaga attgcgtact
    19621 aaaggagaag gccgggatcg agacgctcga aacaggcgcc gcaccttctg gctcaagatc
    19681 catagatatc tcgggttgat tccaggagcg gtcttcgcga tcatcggctt aaccggaagt
    19741 attctggcgt tttggccaga acttgaagtg tggctgcatc ctggtctttg gaaagtcaac
    19801 ccgcaagagg aacaattcga attccgtccc ttggatgaga tcgtggcggc agccaacacc
    19861 gcgattccat ctgatggcac cccctatgcg ctggtgtttc cacgaatgcc ggatacggca
    19921 ttcatggtga gctacagccg cccggcacct aaccctgaac aattggagtg gcatcaagtc
    19981 ttcattgacc cacagacggc acaaatcaaa ggacaacatt tacggcttga tttggagcgg
    20041 ccatggcgcg gtacgttttt tgatttcgtc ctccgtgtac atagtaccct cgccctgggg
    20101 gcgagaggtt caacggtcgt gggagtgctg gctctcctct tgttggtttc tctcttgtcc
    20161 gggctgatcc tatggtggcc gtctttcggt caattgagcg gagccttcac cataaaacgc
    20221 ggagccgggc gtattcgacg tgtttacgat ctgcataaaa cactcggcct ttatacgtcg
    20281 atgctgttgg tagtagccct ctcttccggc ctctatctcg tgtttcccga ctttggaaag
    20341 aacctgatcg gcatgttctc cgccatcacg cttgtcccag aagacctggc gtccagacct
    20401 ttgcctggtg aacaacccat cggctttgat cgggcagcgg ccatcgccaa tggatattac
    20461 gccgatggac ggtttcagtg gatcttcttt ccccagggac aggaaggatt ctatcgaatc
    20521 gtgaaaaagg agccccatga gcgcaccgat atcctaccgg ctcgggcact ctggattgac
    20581 caatacagcg ggcagattct ccacacctat gaccccgaac aattcaccgc aggtgataca
    20641 ttcgagcgat ggctctttcc tcttcacagt ggagaggcct ttggtcttcc cggcaggatc
    20701 ggcattatga tcctggggtt ggtacccacg atcctctttg ttaccggcct gatgcgctgg
    20761 aagcacaaac ggaaaaagcg caaccttccg aagatgtttc gccctaccac ctcaaggaaa
    20821 ggtattgata gccaagcacg cacctaaagc cccttctcat ccgtcattca accatccact
    20881 gtgcgtcatg aattttccag cccacgactt tccctctctg gtgcgcctcg cctccgtctg
    20941 gcccagctac gaggtggaag gatgaaccaa tttaacaagg agtaatgtat gttctgaggc
    21001 tctcacgatg ggttttacag acctttctaa acttagaagg atgattttgt ttgaggtgcg
    21061 gaattcacca ccgcctttcc ggaccaggat ctagggggat ctatattttt gggatctcat
    21121 gagtcatccg gtcttccgtg tgtttcagta aactgttgca tccgtaagca gacattggta
    21181 gaccaccaga cattccttct atatcgttca ttttcaactt acgctcaatc agactgaaaa
    21241 agcaggaaca atccctccat cacatgaggg cgcatcttgt ttcctagagg aaaatcctgg
    21301 gtgatcgacc tcaaatttcg gctttctccg tagattgaaa aagaaccctg acctgaagcg
    21361 tttctgttag gagccaagct tgcatttcac cccaacaggt acgcggcacc atacacacat
    21421 tggactgagg agaattgatg atgaaacaca tgatggcgtt tattctgatg gtcatgcttt
    21481 tgatggtccc aagttttgtg tttgcccacg gcaacgcgat tcatatcttc ggaacggtca
    21541 cagaatccgc acaggaacaa atcgttgtga caactccaaa aggtgaatcc gtcactctga
    21601 taatccagga aaagactatt ttccaactcg atggcattac gacaaacacc gctcgtccgg
    21661 cagtgggaga ccgggttgtg gctgaggcag agaaagaagc ggacgatctt gtcgctcatg
    21721 aaattcgttt tgcgacaccc aagcccaaat agcttgaaag cggcctttcg tccccggtcc
    21781 attcccaaac tgtccaaccc ttcacatcac gaatcctgct tcatatggtt ccgatagatt
    21841 gattggtgat tggcttttga gtaaggtctc gttcctttcc tgatttcttt tggtgctctt
    21901 gttattccag aaaggtttaa cccgatggtc ttcacgcttc tctctccacc agtaccaaga
    21961 acctttcgca tcccatacct cttcgttttg gcatgtcttc tgtgtctacc ttcgcatctc
    22021 caggcaacgg aatcttccgc tcagcctgag gtcgaaacat tcccggaatt gaccgtcaca
    22081 gactcacgca ttcataccga ctatagctgg ctggccgatt ctcttggcca tcgaatggct
    22141 gaactcaatc gctatccaca tgacgcccgc cgcaacggat gggaaggaaa agtcgtgctg
    22201 cgggtgacga tcggagcgga gggccagctc agagacgtca tcgttcagga tagctcaggc
    22261 tacgaaccct tggaccaggc cgcgcaggaa gcggtgcggc tggcctgccc gctcccctta
    22321 caacacaaga tccaccgacc cgaagtcgcc atcactcttc cggtcgttta tcgcctatct
    22381 cactagggcc ttaaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaag
    22501 tcgccatcac tcttccggtc gtttatcgcc tatctcacta gggccttaaa cttcttccct
    22561 gccaataagg attattcaga attttcaccg gcttgttcac caggaaccct ttcactcggc
    22621 atacaaccat caagagttgg gtcgagaaaa agcatccaac tctgttcttt tttttgaaga
    22681 tattttcctt acttccccct gtcactcaga ccggctatga agtctaagct cactccagcc
    22741 atttactctc ggatctggcc cgattatcag acggtctggc gctggcattt ttatgccggc
    22801 ttattttgtg ttccctttat cgtgttgttg gcgattagcg gaggaatgta tttattcact
    22861 ccccagatta atgcctggct cgacgggccc tatgatcgcc ttttccttac tggacccaac
    22921 gccacggcac aggcccaagt ggaagctgcc cttcacgccg tcccgggttc tacattatcc
    22981 gcgtatgaac tgccgaagga tcaggatgac gcgacccgga tcattgtgaa caatcggggg
    23041 gaacgccttc gtgtgtatgt acatcccgaa accctggaaa ttctgaagac cattcctgaa
    23101 gaagaccggt ttaccaggat ggtgtttcat tttcatggag aattattgat gggcaatatc
    23161 ggctctgtct gtgtagaatt agctgcctcc tggacgatca tcatgatcgt caccggcctg
    23221 tacctgtggt ggccacgtgg tcatgaagga attggtggaa tcgtgtatcc ccgattgaac
    23281 cgtggctcac ggggattctg gcgggatctt catgccgtca caggtttctg gatctctctc
    23341 tttgttttgt ttttgcttgt caccggactg ccctgggcga aagtctgggg tgagtatttc
    23401 aaagagattc gtcgtgtgac cggaacggct gtcgtggaac aggattggac ggtcggggca
    23461 acttcccgga acacgccaaa ggatctttca ggggaacatc tcgcccatat tggtcagaca
    23521 attcctcccg ctaacattgt ggaccctccc ttggactatt ctccgatcga tattctcctg
    23581 cccgctgttc atgcgctaga tctcccccct cccgtcttca taatcccccc ttctcacatt
    23641 gaagggtatt ggactgccaa gtcggacact cagaaccgtc cacaacgagt caaagtcatg
    23701 ttggatgcca cgaccggggc cgttctacaa cggaaaaact ttgaagaccg tcatgttttg
    23761 gaccggataa ttggcttcgg tgtagcggct catgaagggc aattgtttgg ttggccgaat
    23821 gtcgtattgg gggtattcac taccgggggt ctcctcatgg tagttacaag cgggattctc
    23881 atgtggtggc gtcgccgtcc ttcgggacta ctgggtgctc ctcccttgcc tcaagctcaa
    23941 cccttttcat gtggattcat gatttttctc ctgttcttct gtctctacct tcccttgttt
    24001 gccgcttccc tgacatcgat actgcttatt gagaaactac tcattcgccg ggttcctcca
    24061 cttgccctat ggcttgggct tgagaaacgc gtctgaagac aatctccctg tcacgtttcc
    24121 agcaatttta attgttcgag ttatttcctt ttccagaaaa actgtagtat gcatatgcat
    24181 ctggtgcggt tgcggccagc actccacatc ttaaggtagc gtatactttc acccttctgg
    24241 tagaaataac ctctctcacg tttaggaaac atgaacgtca tttttcatcc tacattcaaa
    24301 tatctgtgga ggcacccatg ataagaccgg tattttttgg agccacttcc gtggtcctct
    24361 cgattggctt gggaggtgtc gtatccttag ccctcggggc ggaaccatct gtctcgatga
    24421 ttcaggccgg accaaagcac atcatcgacc ataagggcat gcaaaccacc ggccccatgg
    24481 cccaactgga tacagacggg acactgcacc tcgcctgggg tggggaaaag caggaagagc
    24541 gaggtgtctt ctatctcaag gttccacctc aacaggaaga gtttcccgaa ccaaccaaag
    24601 tcaatccctc caatcctccc gccgcttccc tccatgaacc accggcgctg gcgttaggcc
    24661 ataacaacga tgtctaccta acctggacca cccctcatcc gaatgctggc ggaaaaccgt
    24721 ttgccaacct tcttctccta agccgatccg ttgatggagg caagaccttt cttcctccgg
    24781 ttcaagtcaa cgacgacgag gccgtcaccg gccattcttt tgacaatctg accgtgagcc
    24841 cgaatggttc tgttcatatt tcctggcttg atgcccgaga agggaaaaaa gatccggcca
    24901 cctataccgt cgtttcccaa gatcagggcc acactctttc tcccaattta aaaattgacg
    24961 aatccagttg tgtctgctgc cggacccata tgaccactgc ctctgatggc accgtctatc
    25021 tcgcctggcg caaggtcttg cccggagcgg tcagggaaac ggtcgtatcc cgttccaccg
    25081 ataacgggaa atcgttttcc cccccggtca tcgtgggcca tgatcaatgg gtcttcgagg
    25141 gttgcccaca tcgtcccgcc accataggag tcgatcagca aggacgcctc tatgtcacct
    25201 ggtacacgga aggcccggat gatactcccg gtgtatatct ggcatattcc gatgatcagg
    25261 gaaaaacctt taccccccgc cgcttgttaa acatgtcgaa aggcacgttt ccggatcatc
    25321 ctcaactggc cgtgaatcag gcaggccatc tactcgtctc atgggaagag caatctccgg
    25381 tgcgacgtga agtggtgttc acctattccc tcgaccgagg gcaaacattt agttccccga
    25441 ataaggtgaa tgagaagaaa gccaaaagtc cggctgtggc attgaatgac gcgggacagg
    25501 ccgtcattgc ctggtcagag caagtggttt ttccggcatg gagtacggtg gtccagcctc
    25561 tttcttttcc caccccgcag acggctcaat tacacccaat accatgacgc cgctttctct
    25621 gctcgcttca ggaatcctgg gaataaccct tgccatgggg aacccttctt ctgtcccttt
    25681 cactctcagc cgagtcgatc tcagccagag cctccccgcc ttccagctcc gtacattgca
    25741 gggcactccc gtgcattcaa atgaattagc cggtcgagtt ctcatcttga atttttgggc
    25801 aacctggtgt ggcccttgta aggaagaaat gccctcgtta gaacgtcttc gtcaaaaatt
    25861 ccctccagac caattggcca tattggcggt taccaccgat attcgtccac aagaaatcaa
    25921 agtattttgg cagcaactcg agttgcattt tgatgtcctc ctggatgaac aggaagaact
    25981 ctctcaagcg ctcatggttc gaaatcttcc cacaacggtg attgtggata cgcatggaca
    26041 cctgagccaa cgaatcatgg ggcccagaga gtgggatagc cctgaatcgg tcgcccatat
    26101 ccacaacctc ctcaacccga agtagatgca ccagcggttt caaccgattg ccc
//
LOCUS       DAORAQ010000026        26136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_361_length_26136_cov_61.636325, whole genome shotgun sequence.
ACCESSION   DAORAQ010000026 DAORAQ010000000
VERSION     DAORAQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 26136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26136
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_26136_cov_61.636325"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1936)
                     /locus_tag="PKM72_04235"
     CDS             complement(35..1936)
                     /locus_tag="PKM72_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNP60025.1"
                     /translation="MYSSQLYNPKDFVLTLILAGGVFTFDLLLPRGFAEEMLYTGVVF
                     FATQRLPRRMVYVVAIGCTALTIIGFGLTFSTLSGAPSSLSWPFRFPITNRAFCIGAI
                     WAITLLALQRRRALEALRTSEDRFGLVAESIQDYGIVMLDPEGNIASWNAGAAHIFGY
                     LAGDVLGRPHSLLYPPFAIDSGEPTHFLNEARASGSVMKEQWLVRKNGVKFWGHVGIT
                     VLRDETDQLQGFATVMGDLTKQKQEEDVIRALLRLSEKLNSTFVLETLLDELVTEAIQ
                     LVQAHAGFAGLVSGEQLVCEKYFHGQTMKLCHRSWSPGQGLPGWLLLHQTAYLTNRAH
                     EDRQIEQDFRQAFEIHSALSIPILDAKDTLLGCIEVHNKEKTSGFTRFDQQTMIGVSQ
                     VASIAIQNALAYQKLQEAEALHSQLLEKIMTAQEDERRRISRELHDEIGQSLTSLLVG
                     LRAAENDARSDGMAARVNDLRKITSTTLQEVQRLAKGLRPSVLDDFGLEEAIARYGGE
                     FSSTYGIEVDVAQNWQSKNRLSPAVETALYRIVQEALTNIAKYAKATTVSILLQQTPT
                     QIRLIVEDNGQGFDAEAIVGRAAAGAHLGLHGMRERALLLNGSISIESSAGTGTTIYV
                     NIPLKAETA"
     gene            complement(2187..2843)
                     /locus_tag="PKM72_04240"
     CDS             complement(2187..2843)
                     /locus_tag="PKM72_04240"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNP60026.1"
                     /translation="MFSGIIEEMGAIQSINKGLTGAKFSILASLTLEDLKLGDSISVA
                     GACLTASQVGDLGFSVDVSTETLNCTTLGTISVGTPVNLERAMKLNARMGGHIVTGHV
                     DGIGLLRARQQDGNSIQLTIEAPEEIMRYCVAKGSITVDGISLTINAVSERSIAVAII
                     PHTAKVTTMGLKQIGDTVNLESDLIGKYVERLLQTSGMLPAKPAPMINREYLEKRGLL
                     "
     gene            complement(2860..2991)
                     /locus_tag="PKM72_04245"
     CDS             complement(2860..2991)
                     /locus_tag="PKM72_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60027.1"
                     /translation="MNEEMGTGDLIIGIVASLGVVVLIVVFVVIVKKVLLNKDSHIQ"
     gene            complement(3032..4219)
                     /locus_tag="PKM72_04250"
     CDS             complement(3032..4219)
                     /locus_tag="PKM72_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP60028.1"
                     /translation="MKDSKAFLFLSLVGFCSFVSYDMVRRPALALFVESLGAGPFLVG
                     LLVAVSTVTGVILKLPMGLLSDQVNRKRLMLGGVLAFAFPPFLYPFTSDLWTLGALRL
                     VHGLATAMFTPLALAMVAELFAKRRGEAFGWYTSATQGGGLLGPMIGGAVVYQYGFSP
                     TFLLAGVFGLLALVFFWLIPPTQAPSRVHQHSSSTIWKEMREGLRRVCQIPPILITST
                     VEAAKMMGNGTLMAFLPLYGLLIGLNAAEIGVLFGVQALTSFIAKPVMGRISDRGARQ
                     PLIFGGLCLCGLMVMFIPHIQGYILLLVVAGTFGFGEAVVTSSTTALVADFSEGKDLG
                     AGMGLRGTIMDIGHAGGPLLAGILIHALGYGGGFFCIGSLLLLTAAFFGVTMIGIKKP
                     VVL"
     gene            complement(4368..5171)
                     /locus_tag="PKM72_04255"
     CDS             complement(4368..5171)
                     /locus_tag="PKM72_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNP60029.1"
                     /translation="MNGNRVKGQERNYPFLSLIMVGVMWCALMSQQTVHAYQEEEVAG
                     GGIISGVMKFSGIVPSAKTHKVTMGSNPEFCQTISDKNGVIHIPEVRVSSKQRLADVI
                     VFLQEVERGKPVPKEGPVVTVDRCQFAPRVIGAMDEQTLRIAMHDPILHQLRGWEMLD
                     KGRLPLFHLPNLGEGGEATVPLKTRGSSIIKLECDQHRFMQGWLLLAANPYVTVSDDH
                     GVFRLTDVPEGTHTVGAWHPVLGYQEAKVTLSSGRQETLALTWVSPLKP"
     assembly_gap    5631..5730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5746..6093)
                     /locus_tag="PKM72_04260"
     CDS             complement(5746..6093)
                     /locus_tag="PKM72_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP60030.1"
                     /translation="MAIEVIIRGIRGATVVDFHGRLDMHSRWHVKAIINQCCHTEEEH
                     LILNLKGLTFVDSAGLGLLVLCSYQFKELRRRITWIQPQGCVGELLHNLQINELISVC
                     QSEQDALSSVSPS"
     gene            6263..8122
                     /locus_tag="PKM72_04265"
     CDS             6263..8122
                     /locus_tag="PKM72_04265"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60031.1"
                     /translation="MPPAIHPVDLIEILRQHRLTPAIVFLTSRRACDEAIDTFAHSSA
                     RMSSQRSEAIREFLEKFTKDFPSITHHPLIPVVQEFGVAAHHAGHLPSWKIAIEELMR
                     LGLLDAVFATTTLAAGVDFPARTVVITQSSVRKSQDFTDLTNSEIQQLAGRGGRRGKD
                     LVGVVVITPSPYIDLHILGKGLTGYPEPIDSQFVVSYPMVLNLLKAHSLDQIEGLLAK
                     SFAQFQLNQRSGIHQDHLDQIKEQLEPYGPRECADWITQWKGFDLARRRKAHRHPIVT
                     NDTPFLTACLPFLTPGRLIGLPKGPAIILRQYRSRGTFAPMLSVIRAKGTLGECPAHS
                     VTQVYDRVFEFQPSRTIPWCQPQVLSQLKKELSQLPTRLPTVPILPETQETDDPLLAE
                     ILTEEFPCPTCPSHSACKKDFPLASKLRQKSQQLTKTIQALRDGLWHRFQQKADVLHK
                     FGYITASSQLTADGEWARLIRINHSLLITELIRMDAFSGIAPGLLAGIMASISHDDDR
                     PGSYMRLPSGLATMLTQVRAVADSLSPYEPPPLLRSDVAALVESWIGNPQLTWASLVR
                     STSMAEGDVYRLLARTLEFLSQIYGLKITHPGLADTAHSAMVTMRREVLQELP"
     gene            8145..9200
                     /locus_tag="PKM72_04270"
     CDS             8145..9200
                     /locus_tag="PKM72_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNP60032.1"
                     /translation="MHFDDLKHELQSLPVKYLHHMAKGRIHRHFRLGKHRLVELMLAF
                     PPDQILAIETELQQILTTRQERLAAQEARAAARPQKAASPFQSKNRQMKGKPNFGPSQ
                     PSITLQEILKGIGVPEPQPFVPDPWQTEAVEHLAHSDVIVSAPTGSGKTYVAIQAIKQ
                     AMALNQTVIYTSPLKALSNTKFMEFTQLFGPDQVGILTGDRRDNAQAPLLVMTTEILR
                     NLFYDAASGEIDLRLHTLGLVILDESQYLADPERGVVWEETIILCPSQARLLLLSASI
                     GNPQDLAEWLQAIRPIPCHLIRHAKRSVPLRGLYLHPTGQLVPLFKNQDILQGKGYIF
                     HPDTRQLFTEYEIKPFR"
     gene            9455..9982
                     /locus_tag="PKM72_04275"
     CDS             9455..9982
                     /locus_tag="PKM72_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60033.1"
                     /translation="MKTRFLASTCIKYFLVTLLPVIFIGCQSIQPKSPGELAMEDQEF
                     LGIWDAYNQCVDGSDIQRMQANLSVLASAPKPISLDDSPIPVPAFLKKLSTARGSRLA
                     VDPRAMAASCSIHLAEVAQLSSDWPTALRTFQKIIVDYPESQYAFYVSKANHALEQLT
                     TIRPVSLSTQEVLVQ"
     gene            complement(10137..10487)
                     /locus_tag="PKM72_04280"
     CDS             complement(10137..10487)
                     /locus_tag="PKM72_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNP60034.1"
                     /translation="MNSQSSLEPTDMEWLENGVLGITWSDGHKGIYPVRYLRQHCPCA
                     ACTDEWTGELRLKPDSIPLLILLQDMEPVGRYAFRFKWSDGHDTGLYSYTALRSMCQC
                     DICQPNKPKSKQLL"
     gene            complement(10500..11264)
                     /gene="recO"
                     /locus_tag="PKM72_04285"
     CDS             complement(10500..11264)
                     /gene="recO"
                     /locus_tag="PKM72_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNP60035.1"
                     /translation="MPLLRTRAVIIRSQRWGDADRIVTLYSPDLGKIRGAARGARRMK
                     TRFGGVLELFGIVDVTLFQKTPDALGQISQIDLVKNFKAVREDLGVMAAAAKMVRMVE
                     MITADRDPNPEMYSALVYGLESLLPDRDLALATLLFQIHVLSYTGFRPQIDRCTECGN
                     MASSKMSQWFSPRLGGMVCNGCGQDGVGRLLSISKGSLAFIEQARRLPMASLSRLKAI
                     GTVRIEVEEAIEAYFQAVVGKALPTFEQWVSKDAHS"
     gene            complement(11327..12220)
                     /gene="era"
                     /locus_tag="PKM72_04290"
     CDS             complement(11327..12220)
                     /gene="era"
                     /locus_tag="PKM72_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNP60036.1"
                     /translation="MKSGQLAILGRPNVGKSTLLNALVREKIAIVSDKPQTTRNLILG
                     VGHYPEGQLILIDTPGLHTPHHRMNTQMVQSALGTLTQSDVVYMIVDSQVLPGPGDRQ
                     VMEQVTTAHQERPFQGVFLLLNKADAVAKSRLLPLIDQYRTWFPWSEIIPISAKTGVN
                     LPQLVELTFACLPSCPELMYDEDFLTNQPMRHMAAELIREKVLEQTYDELPYAVGVMI
                     EEFQEEPDLTSIRAVILVEKTSQKGIIIGKKGMRLKEIGQSARIDMERLFGMKIYLRM
                     WVKVQEGWRDNVQMLSELGYG"
     gene            complement(12309..12662)
                     /locus_tag="PKM72_04295"
     CDS             complement(12309..12662)
                     /locus_tag="PKM72_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNP60037.1"
                     /translation="MRPNNKKRTSTGFGGDLLDRLPMVGAFLMMSVMAGTMFFNSDGL
                     PLYFHMRETREHLTEQIQQLQDINALLEEDITRIQRDPQRLEELARNRLGMVRQGEVV
                     YQFVEPVPSSFTARP"
     gene            complement(12672..13958)
                     /gene="eno"
                     /locus_tag="PKM72_04300"
     CDS             complement(12672..13958)
                     /gene="eno"
                     /locus_tag="PKM72_04300"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNP60038.1"
                     /translation="MSLIQNITSRMILDSRGNPTIEVDVHLASGMAGRAAVPSGASTG
                     TREALELRDGDKKQWMGKGVSKALAGIQKHILPALRGMEAQDQVSIDSRLIQLDGTKG
                     KTKLGANATLGVSLAVARASAQELGIPLFRYLGGVSARELPVPMMNIINGGAHADNGL
                     DLQEFMIMPVGATSFREALRMGTEIFHNLKAILKSKNMSTAVGDEGGFAPAVRSNEEA
                     LDFIVQAIHQAGYRAGKDVVLALDAAASEFFEKKTYHVRHGVKPKMSSGDMVEYYAKL
                     VKEYPIVSIEDGLDESDWTGWKQLTDHIGDRVQLVGDDLFVTNVEFLGRGIREGIANA
                     ILIKVNQIGTFTETIEAMRMAQRAGYGVVVSHRSGETEDTTIADLAVALNAGQIKTGS
                     LSRTDRLAKYNQLLRIEETLGSSALYRGKAVLPVQA"
     gene            complement(14030..15460)
                     /gene="gatB"
                     /locus_tag="PKM72_04305"
     CDS             complement(14030..15460)
                     /gene="gatB"
                     /locus_tag="PKM72_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNP60039.1"
                     /translation="MAYEPVIGVEVHAQLQTKTKLFCSCPTSFGLPPNTQVCPTCLGL
                     PGSLPVLNRRAVEMAVRTGLALHCEIMLTNQFSRKNYMYPDLPKGYQISQYDLPICGP
                     GWLDISVNGESKRVRICRAHLEEDAGKNVHDVPVEGSGVDLNRAGTPLLEIVTEPDLH
                     SSDEVDTYLKTLHDLLVYLEVCDGNMEQGSFRCEPNVSLRPRGQDRLGTKVELKNINS
                     FRFAKEALEFEISRQTEALTEGETIRQETRLWDVQKGQTVVMRSKEEAHDYRYFPDPD
                     LLPVRVDAEWMESLRQTIPELPAVRMKRFVMEYHLPEYDAKILTDSKNLADYFEACVK
                     LFPHPKTVSNWVMGELLRELNTANTPPESSPVGPEQLVQLLQLVEEGTISLKVARDLF
                     PEFYGSGKSPDQLVKEKGLVQVSDEGILETLIKEVLTQHSTQVEQYRGGKETVLGFLV
                     GQVMKASQGKANPGKVNTLLKQHLTP"
     gene            complement(15505..15819)
                     /locus_tag="PKM72_04310"
     CDS             complement(15505..15819)
                     /locus_tag="PKM72_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNP60040.1"
                     /translation="MAVNQSGASAGSVALAFLSGALLGAVTAIMLAPQPGRDSRERIT
                     GYARRTSEDLRDIKERATDAVEDVVGRGRELVEEISCAVREALDAGRDAMRREREMKS
                     LD"
     gene            complement(15809..16216)
                     /locus_tag="PKM72_04315"
     CDS             complement(15809..16216)
                     /locus_tag="PKM72_04315"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HNP60041.1"
                     /translation="MVDLAAIIIAVAFVVLVGYLVPMILQVKRTVGQSERLLIRLNHE
                     LPGLLKDVKGTNENLLALTDQAKLGIDRATIFLNAVGDVGETVSHVHQTVREKGGGLA
                     VGVTSLLAGFKAATSTMKKRIHKEQKGGPSYGS"
     gene            complement(16231..17703)
                     /gene="gatA"
                     /locus_tag="PKM72_04320"
     CDS             complement(16231..17703)
                     /gene="gatA"
                     /locus_tag="PKM72_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNP60042.1"
                     /translation="MSLFKLTLKELQGKFTAGEVSAMDIARSYLLRLSQVEPKIKAYI
                     TLTPKETLLQQAKAIDDGLKAWRKTQPLMAMPLGIKDNICTEGIRTTCGSRMLETFVP
                     PYDASVMGGLRPHQPLILGKTNLDEFAMGSSTEHSAFGPSRNPWNQKYIPGGSSGGSA
                     AAVAADECVAALGSDTGGSIRQPAACCGVVGLKPTYGRVSRYGLIAFASSLDQIGPIT
                     KTVTDAAILLNAIAGFDPMDSTSANRDVPDFTKSVKKKDVKKLIIGVPREYFAEGLDA
                     EVSEKVQDAILVLQELGGTIREVTLPFTEQAIATYYLVATAEASSNLARYDGVKFGFR
                     SKESGELAEMYRSTRSQAFGPEVKRRIMLGTYALSAGYYDAYYGKAQAVRTLIQREFE
                     AVFQEVDLLVSPVMPTPAFQLGERLDDPLQMYLSDIYTIPASLAGLPAISVPCGLSNL
                     GLPIGLQLMGRPFEEAMVLRAAYAYEQATDWRVKRPVIRS"
     gene            complement(17792..18139)
                     /locus_tag="PKM72_04325"
     CDS             complement(17792..18139)
                     /locus_tag="PKM72_04325"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNP60043.1"
                     /translation="MYRQLLRCKIHRATVTDSSLDYEGSLTIDQDLMDRAGIMAYEAV
                     MVSNLNNGERFSTYALPGARGKGEIILNGPTARLGVVGDKVIIFCYELFNDEEAKRHV
                     PQVIQVDHQNKAK"
     gene            complement(18202..18495)
                     /gene="gatC"
                     /locus_tag="PKM72_04330"
     CDS             complement(18202..18495)
                     /gene="gatC"
                     /locus_tag="PKM72_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNP60044.1"
                     /translation="MASITQKEVEHVAQLARLDLTEAEKPVFAEQLNHILAYVDQLQG
                     VSTEGVPLTASVVHEEPVLREDTPRECLSVEKALANAPLSHNGFFVVPNILVK"
     gene            complement(18673..19404)
                     /gene="radC"
                     /locus_tag="PKM72_04335"
     CDS             complement(18673..19404)
                     /gene="radC"
                     /locus_tag="PKM72_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNP60045.1"
                     /translation="MTSMNEPQLKKGIQSWPERERPRELLLSHGAQRLSDAQLVAILL
                     RIGRPDSSAVDVALDLLTRLKGLRGLANRSLQELCVVPGIGPAKAAQILAAVELGKRA
                     LAIPLTAGLRIHHSQDIYQHYYPLLRDLRHEVFKALLLDAKHGLIRDVTVSEGSLTVS
                     IVHPREVFNLAVRESAAAVIFVHNHPSGDPQPSEEDHALTRRLMAAGEILGIRVLDHI
                     VIGDGRYVSFADEGFLVSSATTLDL"
     gene            19532..20128
                     /gene="rsmD"
                     /locus_tag="PKM72_04340"
     CDS             19532..20128
                     /gene="rsmD"
                     /locus_tag="PKM72_04340"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNP60046.1"
                     /translation="MKPSSIRIVAGELKGRTIPTLPGRTTRPTSQRAREALFSILGGQ
                     IQGATVADLFAGTGAVGLEALSRGATKALFVEQNPLAGANIRRMLSQFKMASSSQVLI
                     EDVSLAIQNSILVGWRPFDMIFMDPPYRLPNVQQILHHLEEADVMAPTGRIIYEHFHK
                     TIPPIPLGKWALIRTARYGDTALTFYRPVPQTEKDSDL"
     gene            20125..20613
                     /gene="coaD"
                     /locus_tag="PKM72_04345"
     CDS             20125..20613
                     /gene="coaD"
                     /locus_tag="PKM72_04345"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNP60047.1"
                     /translation="MTIGIYPGTFDPITRGHTDVIARSLRLVEQLVVAVAPNPRKAPL
                     FDLDERVQLAKIATSDLKGVTVEPFDGLLVNYVRERGALAIIRGLRAVSDFEHEFQMA
                     LLNRKLDSNLETVFLMSSEEYSYLTSSMVKEVASVGGPLHHFLHPEVAECLQGRIRRN
                     AK"
     gene            20610..21803
                     /locus_tag="PKM72_04350"
     CDS             20610..21803
                     /locus_tag="PKM72_04350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNP60048.1"
                     /translation="MNLASRTTRITPSPTLQLSATVKALVANGQQVFDFTAGEPSLDS
                     PEEAKEAAYEAIRSGFTKYTAVTGIDELKEAIIEKFQRDQGLTYSRSQILVSCGAKHT
                     LFNIALALFETGDEVIIPAPYWVSYPEQILVADANPVFFPTSESSGYAIDVKALEAAV
                     TDRTKAIILNSPCNPTGSMYDRQTLEGIAQLALQHNLLIISDEIYEKMIYDQHQHHSI
                     ASLGPDIARNTIIVNGVSKTYSMTGWRIGYAAGPERLIKAMGDIQSQSTSNPSSISQK
                     AAVGALRKSDAFIQHMVKELDIRRKAIVEGLNRIPGIHCPMPSGAFYAFPSVTQLLGR
                     RFQGESLSTPLAFANFFLKEAQVACVPGEPFGSSGHLRFSYTGTLEVIEEGLDHLTKA
                     VAKLE"
     gene            complement(22128..>22451)
                     /locus_tag="PKM72_04355"
     CDS             complement(22128..>22451)
                     /locus_tag="PKM72_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60049.1"
                     /translation="LSELLRRCPGQCICETNQDLLSDEAVVPDPVEALELEDDPDPEA
                     EPAPDPEDPVEDVAGADVDAVEPDPLSELVELVLLSVLVSAVPVSAVFPLFSDLLSGS
                     LSLSE"
     assembly_gap    22452..22551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22668..23252
                     /locus_tag="PKM72_04360"
     CDS             22668..23252
                     /locus_tag="PKM72_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNP60050.1"
                     /translation="MKKMCANGLTKIVGVLFISGICILGVSLEQHALAAGKDSFRVGV
                     LDPQTVIEKSKAGSRALAALKEHAQARETVMKNDQRELETLQEELKAGESRDSKLSEE
                     DQKRKQERFAQKYQAWQKQGQDFQNELGQKQKDLVQEYMKKIEEATAAVAARHGFDVV
                     IDKGSENTLKIVLYNRDGLDLTNEVVKEFDRRFK"
     gene            complement(23413..>23646)
                     /locus_tag="PKM72_04365"
     CDS             complement(23413..>23646)
                     /locus_tag="PKM72_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP60051.1"
                     /translation="QELGCHHIILNFSNITWIDSIGLGQMFLWYHRMKPDRIRLSIVS
                     PQTHVRELLECANLPQVIPIYQSEEEAVGGMTH"
     assembly_gap    23647..23746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23861..26044)
                     /locus_tag="PKM72_04370"
     CDS             complement(23861..26044)
                     /locus_tag="PKM72_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNP60052.1"
                     /translation="MLKFLQIFLVALLLGNLACAADPIPLTIEQKKNALVVFRGQQPI
                     MVIQDIMLDFHSWDDVQLNRKSNSFYRLHLTYRDVANEEEIESMEDSRVIIDIEADQA
                     GLHFSSDADWFSHVNIFMEDLGGHMFGVKQTTEPYNQKSPDLRGLVQDVNAIGEHNRF
                     SSDYATAVSPMFFSTKGYVSFFNTFAEGRYQFAMNGRTELYHAAREFNWHVLDAPTLD
                     HALVNYYQLIGAPKHVPLWGTGPVIWRDVHKRGKQDVLDDTRRFTEMKIPFTGLFVDR
                     PYSDGAHGWSDMNFGRDFSNPREWIKVLHEDYHIHLMTWIAPMTFGEQNFPGRMAGHK
                     GYFDLSNPKAVSEFTRRLTELQYNVGVQGHKMDRADESFPYDESWQDGTPILERRSKF
                     IWLYAKHTHEALADYWGEDQFNFARAAVHGTQPHLSAIWAGDVMTNWEGLRSNMANSI
                     RASYLGFPNWGTDAGGFTGNTGLISEDLYLRWIQFGLWTGFFEIKLDGASGRGNDRAP
                     WRYSESFQDLYRQVFKERMALIPYIYSLLNTAAEQGTLMKPLAGVYPDDEHTYAIWDQ
                     YLFGPSLLVAPIHDQEPIRTVYLPEGSWIDYYNGSIHSGKQWLEVEVVREHVPIFVKA
                     NSLIVKGNMYQGNDRTWSQATPHLDIFYYPQQNATANTFFTLVDAGDTNKHKTIHAKI
                     SGNTVSVSIPGNSYGGKVHIFTSEGSELVRDYPPNKNTIITLELK"
BASE COUNT         6704 a         6909 c         5875 g         6348 t
ORIGIN      
        1 actgcatgat catccacgat aaaaatgcga atcttcacgc tgtctcagcc tttaaaggga
       61 tattgacata gatggtggtt ccggttcccg cagatgattc aatggaaatc gaaccattga
      121 gcaacagcgc tcgctcccgc attccatgca ggcccagatg ggcacctgcc gcagctctcc
      181 cgacaatcgc ttccgcgtca aacccctgac cattatcttc cacaataagt cgaatctggg
      241 taggggtttg ctgcagaagg atcgacacgg tggtggcctt tgcatatttt gctatattcg
      301 tcagcgcctc ctgaacgatt cgatacaagg cggtttccac tgcgggagac aggcgattct
      361 tactttgcca attttgagcc acatccacct ctatgccata tgttgaacta aattctcctc
      421 catagcgggc gatggcttcc tccagtccaa aatcatccag cacgctcgga cgcaaacctt
      481 ttgccagtcg ctgaacttcc tgcagtgtcg tactcgtaat ctttcgtaga tcgttcaccc
      541 gtgctgccat tccatctgaa cgggcatcat tctcagctgc gcggagtcca acaagaaggg
      601 aggtcagcga ttggccaatt tcatcatgta attcccgact gatccttcgc cgctcatctt
      661 cctgagccgt catgattttt tccaataatt ggctatgaag cgcttcagct tcttgaagct
      721 tttggtaggc caaggcattc tgaatggcaa tagaggccac ttgtgaaacc ccgatcatgg
      781 tttgctgatc aaaacgtgta aagccactcg tcttctcttt gttatgtact tcaatacatc
      841 ccaaaagggt atccttggca tcgagaattg gaatgcttaa ggctgaatga atttcaaatg
      901 cctgtcggaa atcctgctca atctgtctgt cctcatgcgc tcgattcgtg agataggcag
      961 tctgatggag gagaagccag ccaggtaatc cctgcccagg ggaccacgaa cgatgacaga
     1021 gtttcattgt ctgaccgtga aaatattttt cacacaccag ctgttccccc gaaaccaacc
     1081 cggcaaaccc ggcatgagcc tggacgagtt gaatggcttc tgtcactaat tcgtccagga
     1141 gggtttcaag aacgaaagtg gaattcaatt tttcactcag acgcagcagg gctcgaataa
     1201 catcttcttc ttgtttttgt ttggtgagat cccccatcac ggtggcaaat ccctgaagtt
     1261 gatcagtttc atcacgtaag acggtgatgc caacatggcc ccaaaatttt accccgttct
     1321 ttcgcactaa ccattgctcc ttcatgacac ttcccgaagc cctggcctca ttcagaaaat
     1381 gagttggttc gccggaatca atggcaaaag gtggatataa cagggaatga ggccgaccca
     1441 gaacatcccc tgcgagatag ccgaaaatgt gtgcggcccc tgcattccaa ctcgcgatgt
     1501 tcccctccgg atccagcatt actattccgt agtcctggat gctttcggcg accaagccaa
     1561 atcgatcctc actcgtccga agggcttcta gcgcccgcct ccgctggaga gccaagaggg
     1621 taatcgccca aatggcgcca atacaaaagg ctctgttcgt gatgggaaat cgaaatggcc
     1681 aactcaagga tgacggtgcc ccgctcaagg tggaaaatgt gaggccaaac cctatgatcg
     1741 tgagtgcggt gcatccaatg gccacaacat agaccatcct tcgtggtaac cgttgtgtgg
     1801 caaagaacac cacgcccgtg tacaacattt cttccgcaaa tccccgtgga agaaggagat
     1861 caaacgtgaa aaccccacca gccaggataa gggttaagac aaaatctttg gggttgtata
     1921 attggcttga atacataagg agaaaaacct gctctcctca tacattggcg gtttcagtga
     1981 atgcctgagt ggggatctac ccggttcgtg gacgatcgag ggcactacag cagccgcacc
     2041 aggcgcaaat aatttcatac agctgaacga ttccccacaa cgttgcggga ataacgatca
     2101 caaagagggt gacgactgca aacaaacgcc aaaggggatg tttggttttt tgtggaagat
     2161 tttgggacat gaaatatata agaaaactat aggagaccgc gtttttcgag atattctcga
     2221 ttgatcattg gggcgggctt tgcgggaagc atcccgcttg tttgtaacaa gcgttcaaca
     2281 tattttccaa tcaaatcgga ttctaaattg accgtatccc caatttgctt gagccccatg
     2341 gtggtaactt tggctgtatg cggaatgatg gctacggcaa tagaacgttc cgagaccgca
     2401 ttgatggtta aactaattcc atccacggta attgagcctt ttgccacgca atagcgcata
     2461 atttcctctg gagcttcaat ggtgagttga atcgaattcc catcttgttg tcgagcacgt
     2521 aaaagaccaa ttccatcgac atggcccgtg actatatgcc cccccatcct ggcattgagt
     2581 ttcatggccc gctccagatt gaccggtgtt ccaacggaaa tggttcctaa tgttgtgcag
     2641 ttcaatgttt cggtcgacac atccaccgaa aagcccaggt cgcccacttg actggccgtt
     2701 aagcaggcac cggcgacact aatactatca cccagtttca gatcttctaa tgtcagagaa
     2761 gccaaaatgg aaaactttgc acctgtcaac cctttattaa tcgactgaat tgcacccatt
     2821 tcctcgatta ttccgctaaa catattccac caattttggt tattggatat gactgtcttt
     2881 attgaggaga acttttttca cgatcacaac aaagaccacg atcaggacta cgacaccgag
     2941 ggacgccaca atcccgatga taagatcacc ggttcccatt tcttcattca tggtgttcga
     3001 ctcctttgcc tgcccatcag gaaaaagctc attatagcac aaccggtttt ttgatcccta
     3061 tcatcgtgac accaaaaaat gccgccgtca gtaataataa gcttccaata cagaaaaacc
     3121 cgcctccata gcctagggca tggataagta ttcccgctaa aagcggtccc cccgcatgcc
     3181 cgatatccat aatggtccct cgtaatccca taccagcccc cagatctttt ccttctgaaa
     3241 aatcagcaac cagggccgtg gtcgaggacg tcaccaccgc ctccccaaat ccaaacgttc
     3301 cggcgaccac taataaaaga atatacccct gaatgtgcgg aataaacatc accatgagtc
     3361 cgcacaggca cagtcctcca aaaatcagtg gttgcctggc cccacgatcg gagatccggc
     3421 ccatcaccgg tttggcgata aaggaagtca acgcctgcac tccaaacaaa accccgattt
     3481 ctgcagcgtt caacccaatg agcaaaccat acagcggcaa aaacgccatc agcgtgccgt
     3541 ttcccatcat cttggccgct tccacggtac tcgtgatgag aatgggtgga atctgacaga
     3601 cccggcggag gccttcgcgc atttccttcc atatggtcga ggaggaatgt tgatgaactc
     3661 gcgaaggagc ctgagtcgga gggatcaacc agaagaagac caaagctaac aatccaaaga
     3721 caccggctaa aagaaacgtg ggcgagaatc catattgata cacgacagca ccgccaatca
     3781 tcggacccag tagcccaccg ccttgggtcg ccgaagtata ccaaccaaag gcttcgcctc
     3841 gccgtttcgc aaagagctct gccaccatcg ccaaggccag cggcgtaaac atggctgtgg
     3901 ccaatccgtg aaccaatcgg agagccccta atgtccaaag atctgaggtg aacggataaa
     3961 gaaagggcgg aaacgcaaag gccagcactc ctcctaacat taaccgtttt cgattgactt
     4021 ggtcggagag caatcccatc ggaagcttga gtatcactcc ggtcactgtc gagacagcca
     4081 ccaataggcc aacaagaaaa ggtcccgcac caaggctttc cacaaacaac gccaaggccg
     4141 gtcgacgcac catgtcataa ctgacaaaac tacaaaatcc gacaagacta agaaagagaa
     4201 atgccttgga atctttcata tcagagagcg aacaaattcg tgagccggtg gaagaaaacc
     4261 gtcattgagg aaaagaaccg aaggttgaaa cggtcaaacc tgacccgcca gaatctttga
     4321 ggaatttaaa gaccgacagg tggtcagctc gattgcctca gaaggcctta gggtttcaag
     4381 ggggacaccc acgtcagcgc aagggtctct tgccgtccag aactgagcgt cactttggct
     4441 tcctgataac ccaacaccgg atgccaggcc ccgactgtat gtgtgccctc tggaacatcc
     4501 gtcaaacgga acaccccatg gtcatcggat acggtcacat acggattggc tgccagcagg
     4561 agccaacctt gcatgaatcg gtgttgatca cattccagct tgataatact gctaccccgt
     4621 gttttgagtg gaaccgtcgc ctcccctcct tctcccagat tgggtaaatg gaaaagtggc
     4681 aggcgtccct tgtccaacat ctcccatccg cgaagctggt gaagaatcgg atcatgcatg
     4741 gctatccgga gggtttgttc gtccatggca ccgatgactc gtggcgcaaa ttgacaacgg
     4801 tccaccgtca cgaccggacc ttctttcggt acgggtttgc cacgctcaac ctcttgaaga
     4861 aacacgatca catcagccaa gcgttgtttg gaagacaccc ggacttctgg aatatgaatg
     4921 acaccatttt tgtcagaaat ggtctgacag aattctggat tgcttcccat ggtgacttta
     4981 tgggtcttgg ccgatggaac aattccagaa aatttcatca caccggaaat tatccctcca
     5041 ccagcaacct cctcttcctg gtaggcatgc accgtctgtt ggctcatcag ggcacaccac
     5101 atcacaccca ccatgatcaa tgataggaat ggataatttc tttcctggcc tttgacccga
     5161 ttgccattca tgacacaaat ctcctcacct ataaatttta tccttaagaa gcctcttgga
     5221 cttccaacgc acatgggacg ggttagtggg ggataacctc tacacatcgt tggagggaac
     5281 gaggatcatt aaatctctcg gagagtctca ctttcagtga ctcaaccatc cagattagcc
     5341 tcactgaatt caagggcact tggtcaattt ttccacaggt tggtacatca aaaaacaacg
     5401 gtacctggca tttagaccaa aatacttcgt ggtgattcct acactccttg gaacaaaatg
     5461 tcatatccac tcgaacggac gcaatatggc cttttgtttt tttcaaggaa cgatcgtttt
     5521 ttctgtacct cttgaggtat tttctgacag aaatcatctt gccgttttct atgtcaggct
     5581 ggcaagagta aagaactctc atttttgcta aagaacggag gatattggtt nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaatgagga ggatttcagg aaggagagac
     5761 tgaagataat gcgtcctgtt cggactggca cactgagatg agttcattga tttggagatt
     5821 atgaagcaat tctccgacac atccctgcgg ctgaatccag gttatgcggc gacgtaattc
     5881 tttgaattga taggagcaca acacgagaag acctaaccca gcgctatcca caaatgttaa
     5941 gcctttgagg ttcagaatca ggtgctcttc ttccgtatgg caacattgat taataatggc
     6001 tttgacatgc catcgggaat gcatatccaa ccggccatga aaatccacga cggtggcgcc
     6061 tcgaattccg cgtataatga cttcaatggc cataatcgaa accttatcgg tccaatatgc
     6121 cagaccttaa cgacgggtgt ttcctgcctt ctcagcttcc gcgatccatg gtaaccccac
     6181 agagcacggt tcgttttctt ttattcgacc tgtatcatat tagtgttttc atccaccgac
     6241 tcctgctaca atttcaccat ccatgccacc agccattcat cccgttgatc tcatagaaat
     6301 tctccgccag caccggttga ctccggcgat tgtctttttg acatctcgcc gggcctgcga
     6361 tgaggccatt gatactttcg cccacagttc agccaggatg tcctctcaac gctcagaggc
     6421 catccgggaa tttctggaga aattcaccaa agacttcccg agtattaccc atcaccccct
     6481 cattccggtt gtgcaagaat ttggagttgc ggcgcatcac gcaggacatc tcccatcctg
     6541 gaaaattgcg attgaagaac tcatgcgcct gggattgttg gatgccgtgt ttgccacaac
     6601 gaccttggcc gccggggtcg acttcccggc acgtaccgtc gtcattaccc aatccagcgt
     6661 ccgtaaatcg caggatttca cggatttgac gaacagcgaa attcaacaat tggccggacg
     6721 cggaggccgc cgaggcaaag atttggtggg agtcgtggtc attaccccct ccccgtatat
     6781 tgacttgcac atccttggaa aaggactcac aggctatccg gaaccgattg acagtcaatt
     6841 cgtggtgtcc tacccgatgg tcctaaatct gctaaaggcc cattcgctgg atcaaattga
     6901 ggggctgctc gctaaaagct ttgcgcaatt tcaactgaat caacgatccg gcattcatca
     6961 agatcatctg gatcagatca aagaacaatt ggaaccctac gggcccaggg aatgcgccga
     7021 ttggatcacc caatggaagg gctttgatct ggcccgtcga cgcaaagcgc accgacatcc
     7081 catcgtcacc aatgacacgc cattcttgac tgcttgtctg ccgtttctga ctccaggtcg
     7141 cctcatcgga ctgccgaaag gtccggcgat tatacttcgt cagtatcgaa gccgtggaac
     7201 ctttgcccct atgttatccg tgattcgggc caaaggcacg ttgggtgagt gtcccgctca
     7261 ttcggtcact caggtttacg accgggtgtt tgagtttcag ccctctcgca ctattccctg
     7321 gtgtcagcca caggtcctct ctcaactcaa gaaagagctt tcccaattgc ccacgcgtct
     7381 gccgacagtc cccattttgc cggaaaccca ggaaacagac gacccgctcc ttgcagaaat
     7441 attaacagag gaatttccct gtcccacctg cccgtcccac tcggcatgta agaaagactt
     7501 tcccctggct tcgaaactcc gacagaaatc ccagcaatta accaaaacga ttcaagcact
     7561 ccgtgacgga ttgtggcacc gatttcaaca aaaagccgat gtgctccaca aatttggata
     7621 tatcaccgcc tcctcgcaac tcactgctga cggagagtgg gctcgactca ttcgtattaa
     7681 ccattccttg ctcataacag aactcatacg tatggatgcc ttctcaggca ttgctccagg
     7741 attgttggca ggcatcatgg cgagtatttc ccatgatgat gatcgtccgg gatcctacat
     7801 gcggctccca tccggcctgg ctacgatgtt gacgcaagtg cgggcggtgg ctgattcatt
     7861 gagtccgtat gaacccccac ccttgttacg ctcagatgtc gccgcactgg tggaaagctg
     7921 gatcgggaat cctcagctca cttgggcctc actggttcgc tccacatcca tggccgaagg
     7981 ggacgtgtat cgcctcttag ccaggacttt agaatttctc tctcaaatat atggactcaa
     8041 gatcactcac ccgggcttgg ccgataccgc ccactccgcg atggtgacca tgcgacggga
     8101 agtcttgcag gaacttccat agccaaagat ggggacccaa cgccatgcat tttgatgatc
     8161 tcaaacatga actccagagt ttaccggtga aatatttgca tcatatggcc aaaggccgca
     8221 tccatcgaca tttccgcctc gggaaacatc ggcttgtgga attaatgctg gctttccctc
     8281 ccgaccagat tctggccatt gaaactgagt tacaacaaat actgactact cgacaggaac
     8341 gactggccgc acaggaagcc cgcgcggctg cccgccctca aaaggccgct tcgccctttc
     8401 aatcaaaaaa tagacaaatg aagggaaagc ccaacttcgg cccttcccag ccatcgatta
     8461 cgcttcagga aattttaaag gggatcgggg ttcctgagcc tcagcctttt gttccagatc
     8521 catggcaaac cgaagccgta gagcatctgg cacattccga tgttatcgtc agtgctccga
     8581 cgggaagtgg aaaaacctat gtcgccatac aagcgatcaa acaggctatg gcactcaacc
     8641 aaacagtgat ttacacctct ccactgaaag cgctttcgaa tacaaaattc atggaattca
     8701 cccaactctt tggaccggac caagtcggga tattaaccgg agaccggagg gacaatgccc
     8761 aagctccgct attggtaatg acgaccgaaa ttctaagaaa tttattctat gatgccgcta
     8821 gtggggagat tgatttacga ttgcatactc tggggttagt cattctggat gaatcgcagt
     8881 acctcgctga tccagaacga ggggtggtgt gggaggaaac gattatcttg tgtccctcac
     8941 aggcacggct cctcctcctc tctgcatcta tcgggaaccc tcaagatttg gccgaatggc
     9001 tacaggccat caggcctatt ccctgccatc ttatccgaca tgccaaacga tccgttcctc
     9061 ttcgagggct gtatctccat cccacagggc aactcgttcc attattcaaa aaccaggata
     9121 ttctccaagg caaaggctat atatttcatc cagacaccag acagctgttc accgaatacg
     9181 aaattaaacc atttcgataa tttatgaatt agtgggaaaa attacatcgc ctgtaatctc
     9241 aagtgtgaca ttaaatttca agggaccaga aactgtgccg aaataaacac atgttgtggt
     9301 aataagtata cgcttccgcc atcattctat tgcttttatt gttaatttta aggaaataac
     9361 ccatatccat aattattatt tttgagcatg aattttgcgt taataatatt gaattcctaa
     9421 aatgtttttt aaacttcgat ttccaggctc aaagatgaag accagatttc ttgcttccac
     9481 atgtatcaag tatttcctcg taaccttact tcctgtcatt tttattggct gtcagagtat
     9541 tcagccaaaa tcacctgggg agttggccat ggaggatcag gaattcctgg gaatttggga
     9601 cgcctataat caatgcgtag atggttctga tattcaaaga atgcaggcca atctttccgt
     9661 gttagcctct gcacctaaac cgatctcact ggatgattct cctattcctg tccccgcctt
     9721 tttaaaaaaa ttatcgacag cgaggggttc ccgtttggca gtcgatccac gggccatggc
     9781 tgcctcatgc tcaatccacc ttgcagaagt tgcccaattg tcatcagact ggccaacggc
     9841 tctgcgcacc tttcagaaaa tcatagtaga ttatccagaa tctcaatatg cattctacgt
     9901 cagcaaggcc aatcatgcgt tagaacaatt gaccaccatt cggccagtct cactatctac
     9961 acaggaagtc ctggtacagt aaaaatcgtt ccctcctggt tcacttactc aagccttttt
    10021 ttgcttcggt cgtccgcttc acgctttctt tcgtaacggt cctcccttta aatattttat
    10081 aggttcatac tggcactatc tcgctggaat cttgatgaaa agaaaaccag aagaagctag
    10141 aggagttgct tgctttttgg cttattgggc tggcaaatat cacattggca catgctgcgc
    10201 agtgcggtgt aggaatagag cccggtatca tgtccgtcac tccatttaaa tcgaaaggca
    10261 tagcgcccaa ccggctccat atcctgcaat aaaataagaa gggggataga gtccggcttg
    10321 agacgcaact caccggtcca ttcgtccgtg caagctgcac aggggcaatg ttgtcgcaaa
    10381 taccgaactg gatagatgcc cttatgccca tcactccagg taatgcccaa caccccgttt
    10441 tccagccatt ccatatctgt aggttcaagt gatgattgag aattcatacc cgattatcct
    10501 tatgagtggg catccttcga cacccattgt tcaaatgttg ggagagcttt tccgacaact
    10561 gcctggaaat aggcttcaat agcttcttcc acttcaatac ggaccgttcc aatcgctttc
    10621 aatcgagata gactggccat ggggagtcgt cgagcctgtt caataaacgc caggcttcct
    10681 ttagaaatgg ataataatcg gcctacccca tcttgaccac agccgttaca gaccatccca
    10741 cctaatcgag gggaaaacca ctgagacatt ttggaggagg ccatatttcc gcattcagta
    10801 caacgatcaa tttgtggacg aaaaccagta tagctcagca catgaatttg aaataataat
    10861 gtggcgagtg ccaggtcccg gtcaggaagc aggctttcca ggccataaac cagtgctgaa
    10921 tacatctcag gattgggatc acgatccgcc gtaatcatct ccaccatacg taccatcttg
    10981 gcggctgcag ccattacgcc caaatcttct cgtacggctt taaagttttt gacaagatct
    11041 atttgactaa tttgtcccaa agcgtcaggg gttttctgga acagggtgac atcaacaatc
    11101 ccaaagagct caagaacccc gccaaaccgt gttttcattc ggcgagcacc acgagcggcg
    11161 ccacgaattt tacctagatc aggggaatac agggtaacaa ttctgtcagc atcaccccag
    11221 cgttggcttc gaataatgac cgcgcgggtt cttaggagcg gcatgagaac tccacgagga
    11281 ctctctgcac gtcagacgta aaaactactg tgattaccac ccatgttcat ccatatccca
    11341 attcgctcaa catctgcaca ttatcgcgcc agccctcctg cactttcacc cacatgcgca
    11401 aataaatctt cataccgaac agtcgctcca tatctatccg cgcactttgg ccaatctctt
    11461 tgaggcgcat gccttttttc ccgataatga ttcctttttg actggttttt tccaccagga
    11521 taacggcccg aatacttgtc aggtctggtt cttcctgaaa ctcctcaatc atgacaccca
    11581 ctgcatacgg caattcatcg taggtctgtt ctagaacttt ttcacgaatg agctccgcag
    11641 ccatatgacg cataggctga ttggtcaaaa aatcttcgtc atacatcaat tccggacatg
    11701 agggcaaaca cgcaaaggtt aattcaacta attggggcag attaaccccg gtcttagcgg
    11761 aaatggggat aatttcactc cagggaaacc acgtgcggta ttgatcaatg agagggagca
    11821 gcctggattt ggcgaccgca tcggctttat ttaataataa aaatactccc tgaaatggtc
    11881 gttcttgatg ggctgtggtc acctgctcca tgacctgtcg gtctccgggg cctggcaaaa
    11941 cctgactgtc cacaatcatg tagaccacat cagattgagt cagggtgcct aacgctgatt
    12001 gcaccatctg tgtattcatt cgatggtgag gcgtatggag gcctggtgta tcaatgagaa
    12061 taagctggcc ttccgggtaa tgcccgactc ctaaaatgag gtttcgcgta gtttgtggtt
    12121 tatcagagac aatggcgatt ttttcgcgaa ccaatgcatt gagcaacgtc gatttgccga
    12181 cattgggtcg gccgagaatg gctaattgac ctgatttcat cgaataactt ttaagtatgg
    12241 gaaaacggaa gaaacatcaa tgcatgagta tgaagggttg gaggggcctg gcgcaagaag
    12301 tctggccgct aaggtctggc cgtaaatgag gaagggaccg gctccacaaa ttgatacacc
    12361 acctctccct gccggaccat gcccaaacga ttacgggcca actcttccag tcgttgcgga
    12421 tcacgctgaa tacgggtgat atcttcttcg agcagggcat tgatatcttg taattgctgg
    12481 atttgttcgg tcaaatgctc acgcgtttct cgcatatgaa agtataaggg caatccatca
    12541 gaattaaaaa acatggtccc cgccattaca ctcatcatca aaaatgcccc aaccatggga
    12601 aggcgatcca acagatcacc cccaaacccg gtggacgtcc gttttttatt attgggacgc
    12661 atacttgaaa atcatgcttg tactggcaga acggctttcc cgcgatataa ggcagatgag
    12721 cctaatgtct cctcaattct gagtagttgg ttatattttg ccagacgatc ggtccgagac
    12781 agggatcctg ttttaatctg tccggcattt aacgccacag ctaaatcggc aattgtcgta
    12841 tcctcagtct cgccggatcg gtgggatacg accactccat acccggcccg ctgagccatg
    12901 cgcatggctt caattgtttc cgtgaacgtg ccgatttgat tgacttttat caagatcgca
    12961 ttcgcaatgc cttcacgaat tcctcgtccc aaaaactcga cgttggtcac aaacaggtca
    13021 tcgccaacaa gctggactcg atcacctatg tgatccgtca gttgtttcca acctgtccaa
    13081 tcactttcat caagcccgtc ttcaattgag acgatcgggt actccttcac taatttggca
    13141 taatattcga ccatgtcccc cgaagacatt ttgggtttaa ccccatgacg cacatgatac
    13201 gtctttttct caaaaaactc gctggccgcc gcatcaagtg ccaacaccac atctttccct
    13261 gctcgatagc cggcttggtg aatcgcttga acaataaaat ccagggcttc ttcatttgat
    13321 cgaacagccg gggcgaatcc accttcatcc cccaccgccg tgctcatgtt ttttgatttc
    13381 aagatggcct tgagattgtg gaaaatctcc gttcccatcc tgagggcttc cctgaaactg
    13441 gtcgccccca ccggcatgat catgaattct tggaggtcca gaccattgtc tgcatgagcg
    13501 cctccattga taatattcat catgggcacc ggtaattctc gagccgagac tccaccaaga
    13561 tagcgaaata agggaattcc aagttcctgt gccgaggccc gggccacggc taacgacacg
    13621 ccaagtgtcg catttgcccc taacttggtt ttccccttgg ttccatctag ttggattaag
    13681 cgggaatcaa tactcacctg gtcctgggct tccatgcctc taagagccgg gagtatatgc
    13741 ttttgaattc cggctaaggc tttagacact cccttgccca tccattgttt tttatcccca
    13801 tcccgtaact ctaaggcttc gcgtgtccca gtggaagctc cggaaggaac agcagctcga
    13861 ccagccatgc cgctggcaag atgcacatcc acttcaattg ttggattccc gcgagaatcc
    13921 agaatcatcc gtgaggtaat gttctgaata aggctcatag aagctgtaat tccttgtaat
    13981 ggtgagtaat ggcgacctct ttatcttgta ccctgagtcg agccttagat caaggcgtga
    14041 gatgctgttt caacagagtg ttgaccttgc caggattggc ttttccctgc gacgccttca
    14101 tcacctgtcc gactaaaaat cctaacaccg tctccttccc accacgatat tgctccactt
    14161 gagttgaatg ctgggtcagc acctctttaa tcagtgtttc caggatgcct tcatcggaaa
    14221 cctggaccag gcccttctct ttgaccaatt gatccggaga ttttccacta ccataaaatt
    14281 ccgggaaaag atctctggca actttcaaac taatggttcc ctcttctacc aattgtaaga
    14341 gttgaaccaa ttgttcgggg cctaccgggg aggattcggg aggggtatta gcggtattga
    14401 gctcacgaag taattcgccc atcacccagt tactgaccgt tttgggatgg ggaaagagtt
    14461 tcacacaggc ttcaaaatag tcggctaagt tttttgaatc cgtcagaatc ttcgcatcat
    14521 actcaggaag atgatattcc atcacaaacc gtttcatgcg gacagccgga agttccggaa
    14581 tcgtctggcg cagcgattcc atccactccg catccacgcg aacggggagc agatccggat
    14641 ctggaaagta ccggtaatca tgggcttctt ccttactccg catgacaacc gtttgtcctt
    14701 tctgcacatc ccacagtcgg gtctcctgac gaatcgtttc accttcggtt aaggcctcag
    14761 tctgcctgga aatttcaaat tccaatgcct ctttggcaaa acggaaagag ttaatatttt
    14821 tcaattcgac cttggttccg agacggtcct ggcccctggg acggagcgac acattgggct
    14881 cacaacgaaa acttccctgt tccatgtttc catcacagac ctccagatag accaagagat
    14941 catgcagtgt tttcaaatag gtatcaacct catccgatga atgcaggtcg ggttcggtga
    15001 caatttctaa caatggagtt cccgcccgat ttaaatcgac accactgcct tccacaggca
    15061 catcatgaac attttttccg gcatcttcct ccaaatgggc ccggcaaatg cgcacgcgtt
    15121 tcgactcgcc gttgaccgaa atgtcaagcc atcctggtcc gcagatcggc aaatcatact
    15181 gcgaaatttg atagcccttc ggcaaatcag ggtacatgta atttttccgg gagaattgat
    15241 tcgtcagcat gatctcgcag tgaagcgcga gtcccgtccg cacggccatt tccaccgcgc
    15301 ggcgattgag caccggcagg cttccaggca accccagaca ggttggacac acctgcgtat
    15361 tcggaggaag tccgaaagaa gtcggacagc tacaaaacaa tttcgtcttc gtttggagtt
    15421 gggcatgcac ctcaactcca atgacaggtt cataagccat aacgcagcag agccactcct
    15481 ctcgatttcg tctttttttg aatcttaatc caaggatttc atttcacgtt cacggcgcat
    15541 ggcatcccga cccgcgtcta acgcttctcg cacggcacag gaaatttctt ccaccagttc
    15601 acggccacga ccgacgacat cttcaacagc atccgtggcc cgctccttaa tgtctcgcaa
    15661 atcttcactc gtgcgtcggg cataccccgt aattcgttct cgggaatctc ttcccggctg
    15721 aggggctaac atgatcgccg tcacagctcc caatagcgct ccacttaaaa aggccaacgc
    15781 aaccgagcct gctgatgcac ctgactgatt aactgccata ggatggccct cctttttgtt
    15841 ctttatgtat acgtttcttc atcgtcgaag tcgctgcttt gaatccggca agtaaactgg
    15901 taacgcccac agcaaggcct ccccctttct cacgaacggt ctgatggaca tgactcacgg
    15961 tttccccaac atctccaact gcattcaaga aaatagtggc acgatcaata ccaagcttgg
    16021 cttgatcggt aagagctaaa aggttttcgt tggttccctt gacgtccttc aataatcccg
    16081 gcaactcatg gttcaagcga ataagtaaac gttctgattg cccaaccgta cgtttcactt
    16141 gaagaatcat cggaaccaga tagcccacca agacgacaaa ggccacggca atgatgatcg
    16201 ctgccaaatc caccatatct cgctatcctt tcaagaccga atcactggcc gtttcactcg
    16261 ccagtcggta gcttgttcat aggcataggc tgcacgcaag accatggctt cctcaaaggg
    16321 acgacccatc agttgtaatc caatggggag gccgagatta ctcaatccac agggaacgga
    16381 aattgccgga agtccagcca aactcgcagg aatggtatag atgtcggata aatacatctg
    16441 caagggatcg tccaatcgtt cccctaattg aaaagccggc gtcggcatga ctggactcac
    16501 cagcagatca acttcctgga acactgcctc aaattcccgt tgaatcaagg tgcgaaccgc
    16561 ttgggctttt ccataatatg cgtcgtaata cccggcactc agcgcataag tccccaacat
    16621 aatacgccgt ttcacttcag gcccaaaggc ttgagaccga gtgctccgat acatctctgc
    16681 cagctctccc gactccttgg aacgaaaccc aaacttgacg ccgtcatagc gagctaaatt
    16741 agagctggct tctgccgtgg cgacaaggta ataggtcgca atggcttgtt ccgtgaaggg
    16801 caacgttacc tcgcgaatcg ttccgcccaa ctcctgcaac accaggatgg catcttgaac
    16861 tttctcggag acttcagcat caagaccttc tgcaaaatat tcccgcggca ctccgataat
    16921 aagttttttg acgtctttct ttttcacgga tttggtgaaa tccgggacgt cacgattagc
    16981 agacgtggaa tccattggat caaatccggc aatggcattg agcaggatag cagcatcagt
    17041 cacggttttg gtaataggac cgatttgatc cagagatgag gcaaatgcga tgagcccata
    17101 ccgggacaca cgcccatacg ttggcttcaa ccccaccacc ccgcaacagg ccgccggttg
    17161 tcggatcgac cctccggtat cggaacccaa ggctgccaca cattcatctg cagccaccgc
    17221 ggcggccgat cctccactgg atcctccggg aatatacttt tgattccacg gatttctgga
    17281 cggcccgaac gctgaatgtt ccgtggacga acccatggca aattcatcaa gattggtttt
    17341 gccaagaatc aggggttggt gaggtcgaag accgcccatc acggaagcgt catacggcgg
    17401 aacaaaggtt tccaacattc gtgatccaca agtcgtccgg atcccctctg tacaaatatt
    17461 atctttaatg cctaatggca tggccatgag cggctgtgtt tttcgccagg ctttcaagcc
    17521 atcatcaatc gctttggctt gctgaagcag agtctctttc ggtgtcaggg taatataggc
    17581 tttaattttt ggttctactt gactgagtcg caacaaatag gacctggcga tatccatcgc
    17641 actcacctcg ccagcggtaa atttcccctg aagctctttt agcgtaagtt taaatagaga
    17701 catacaaacc agaatctggg atcagtggat aaatagggag atggcacgaa aacggccttt
    17761 aaccggagca acccgtttca tacgggattg gttactttgc tttattctga tgatcgactt
    17821 ggataacttg agggacgtgc cgtttggctt cttcgtcatt aaataattca taacaaaaaa
    17881 taatgacctt atcgccaaca actcccaacc tggcagtggg accatttaag atgatctcac
    17941 ctttgccacg ggcaccaggg agcgcgtaag tcgaaaaccg ttctccgtta ttcaaattgg
    18001 agaccatcac cgcttcatag gccatgattc ccgctcgatc catgaggtcc tggtcaatcg
    18061 ttaaactacc ctcatagtcg agactggaat cggtaacggt cgcccggtga attttacatc
    18121 gtaacaattg tcgatacata ttaaatccta atttgtttag tcctgctcgg aaacgagcgt
    18181 ttttggttaa tcaggtcgcc cttatttcac taaaatattc gggaccacaa aaaatccatt
    18241 atgtgacaat ggggcattgg ccagcgcctt ttccacggac aagcattcgc gtggggtatc
    18301 ctcacgtagc accggttcct catgaacaac agaagccgtg agaggaacac cttccgtcga
    18361 caccccttgc aattgatcca catacgctaa aatatgattc aattgttccg caaacacggg
    18421 tttttccgcc tccgtgagat ctaaacgggc taattgggca acatgttcga cttctttttg
    18481 agtgatggag gccatatctg atccttatga gcgcaggaat ttaaaaacca tgctgaaaca
    18541 ttgcaagcaa aaaccacatg ccaccacgta tttctaccat gaaattctca tgaaaagcta
    18601 cttaaacaaa ggatcgaagc gagggtggga aagggagtga ctaggaaaat ccagagaaag
    18661 aacttgaggc aatcacagat ccaaggtggt tgccgaggaa accagaaagc cttcatccgc
    18721 aaagctcaca taacgcccat ctccaatcac aatatgatcc aatacccgga tacccaatat
    18781 ttcccctgcc gccatgagtc gacgggtcaa ggcatggtct tcctcgctgg gctgcgggtc
    18841 accacttgga tgattatgga caaagatgac tgccgccgca gattctcgta cggccaggtt
    18901 gaacacttcc cgcggatgaa caatacttac cgtcaaactt ccctcggaaa cggtgacatc
    18961 tcgaataagc ccatgcttgg catctagcaa cagtgcctta aagacctcat gccgaaggtc
    19021 tcgcaacaaa ggatagtagt gctgataaat atcctggcta tggtgaattc gtaatcctgc
    19081 cgttaacggg atcgctaaag cccgtttccc caactcaacc gctgcgagaa tctgggcggc
    19141 ttttgcagga ccaatacccg ggaccacaca cagttcctgc aaacttcgat tggctaaacc
    19201 gcgcagtccc ttaaggcgcg tcaggagatc caatgccaca tcaaccgccg aggaatcggg
    19261 tcgaccaatt cgtaagagga tggccaccag ctgagcatct gataatctct gagccccatg
    19321 gctcaggagg agttcgcggg ggcgttccct ttccggccag gattgaatcc cttttttgag
    19381 ttgcggttca ttcatactcg tcatgccagt ggggcccata cgaacatgcg ttgaactata
    19441 cgcagctatt ttttaccccg tcaaaaacca tttccccacc ctgtgataga gattgttgag
    19501 gtttccaaaa ctatgatatc gtccactcgc catgaaaccc tcttctatcc ggatcgttgc
    19561 aggagaattg aaaggtcgaa ctatccctac ccttccgggg cgaaccaccc gtccgacctc
    19621 ccagcgagcc agggaggccc tcttttctat cctgggtggc cagattcagg gtgccaccgt
    19681 ggccgatctg ttcgcgggga caggagcggt gggactggaa gccttaagcc gtggagcgac
    19741 caaagcgctc tttgttgagc aaaatccttt ggccggagcg aatattcggc ggatgctttc
    19801 ccaatttaag atggcctcat caagccaggt cctcattgag gatgtgtcac tcgccataca
    19861 aaattcaatt ttggtaggat ggcgtccgtt tgacatgata ttcatggacc ctccttaccg
    19921 gctcccgaat gttcaacaga tcttgcatca cttagaggaa gcggacgtca tggccccgac
    19981 cggtcgaatc atttatgaac atttccataa aaccatcccc ccgattcctc ttgggaaatg
    20041 ggcgctcatc cgaaccgctc gctatgggga tacggccttg acgttttacc gacctgtgcc
    20101 ccagaccgaa aaagattctg acctatgacc attggcatat atccaggcac atttgatccc
    20161 attacccgag ggcataccga cgtcattgct cgaagcctca ggttggttga gcagttggtc
    20221 gtggcggtag ccccaaaccc tcggaaagct cccttgttcg accttgacga acgagtacaa
    20281 ttggccaaaa ttgccacaag cgacctgaag ggagtcacgg ttgaaccgtt tgatggatta
    20341 ctggtgaact acgtgcggga acgtggggcc ttggccatca tccgggggct acgagccgtg
    20401 tcggatttcg aacatgaatt tcaaatggcc ctgctgaatc gaaaacttga ctctaacctg
    20461 gaaaccgtct ttttgatgtc gagtgaggaa tattcgtatc tcacttccag catggtcaaa
    20521 gaggttgcct cggttggtgg tcccctccac catttcttac atcccgaggt ggccgaatgc
    20581 ctccagggcc gaatacggag aaatgccaaa tgaatcttgc ctcgagaacg acccgtatta
    20641 ccccctcccc tacgttgcaa ctctccgcaa ccgtgaaagc cctggtcgcg aatgggcaac
    20701 aggtcttcga ttttacagcc ggagaacctt ctttagattc ccccgaggag gccaaagaag
    20761 cagcctacga agcgattcgc tcaggcttta ccaaatatac tgccgtgaca ggcattgatg
    20821 aattaaaaga agccatcatt gaaaaatttc agcgggacca gggtttaacc tattcgcgat
    20881 ctcaaattct ggtgtcatgt ggggccaaac acacattatt taatatcgcg ctggccttgt
    20941 ttgaaactgg agatgaagtc atcattcccg ctccgtactg ggtctcgtat cccgaacaaa
    21001 ttctcgtggc cgatgccaat cctgtctttt tccccacttc cgaatcctca ggctatgcca
    21061 ttgacgtgaa ggcgctggaa gcggcagtga ccgaccgcac aaaagcgatc atcctgaact
    21121 ctccctgcaa tccgaccggc agcatgtatg atcgacagac cttggagggc attgcccaat
    21181 tggccctcca acataacctg ctcattatct ctgatgaaat ttacgaaaag atgatttatg
    21241 accagcatca acaccacagt attgcctcgc taggcccgga catcgccaga aacaccatta
    21301 ttgtgaacgg ggtatcaaaa acctattcca tgacaggatg gcgaattgga tacgccgccg
    21361 gcccggagag gctgatcaaa gccatgggag acattcaaag ccaaagtacc tcaaacccca
    21421 gctccatttc gcaaaaggcc gccgttggag ctctccgaaa gtctgatgca tttattcaac
    21481 atatggtaaa agagttggat attcgacgaa aagccattgt ggaggggtta aataggatcc
    21541 cgggcattca ctgtcctatg ccttcaggcg ccttctatgc atttccaagc gtcactcaac
    21601 tccttggccg acgcttccag ggagaatccc tgtccacacc gttggctttt gccaactttt
    21661 tcctcaaaga agcccaagtc gcgtgcgtac ccggagaacc atttgggagt tccgggcatt
    21721 tgcgattttc gtataccgga acattagagg tgattgagga gggattagac cacctgacca
    21781 aggccgtggc taaacttgag tagccgacct ctccccgttc ggtggttcgt tttcccataa
    21841 ttccatcacc acgaaacctt gacctgaaca gaatcagcct tatccttatg aacatatgtg
    21901 tgcaagtctc tttaattgac gtttactctc tggaggtatg ccgtgcccat ttatgaatat
    21961 caatgcaccg gtagcggtga acgatttgaa attcaacaga aaatgtcaga tcctcccatt
    22021 caaacctgcg atcaattgtc ctgtggttgt ggcaaagtcg aaccggtcac aaaagtcatc
    22081 tcagcgcccg ccatcatgtt caagggaaag ggctggtatg tcacggacta ctcggacaaa
    22141 ctcaaagagc cagagagtaa atcggagaac aaagggaaaa ccgctgacac cggcacggct
    22201 gaaactaaaa ccgacagcaa gaccaattcg accagctctg atagtgggtc cggttccaca
    22261 gcatccacat ccgcacccgc cacatcttct accgggtctt cgggatcagg agcaggttca
    22321 gcttcaggat cggggtcatc ttccagttca agtgcctcca ctggatccgg gacgactgct
    22381 tcatccgata gtaagtcctg atttgtttcg cagatgcatt gccccgggca tctgcgcaac
    22441 agctctgaca annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngactcttga
    22561 cagctccgct taccctcgtt actctcatca ggtttacccg taattcctgc ctcttcggct
    22621 actctggccg aaccgagggt catcctttaa acgctagagg gaaaagcatg aagaagatgt
    22681 gtgcaaatgg tttgacaaaa attgttgggg tcctgttcat ctccggcatc tgtatcctag
    22741 gtgtgtcttt ggaacagcat gcccttgctg ctggaaaaga ttccttccgc gtgggtgtcc
    22801 ttgatcctca gaccgtgatc gaaaaatcga aggccggaag ccgggccctg gctgcgttaa
    22861 aagaacatgc ccaagccaga gaaaccgtga tgaaaaatga tcaaagagaa ttagagaccc
    22921 tccaggagga actcaaggct ggagagtcca gggactccaa gctgagtgaa gaagatcaaa
    22981 aacgcaaaca agaacgtttt gcccaaaagt atcaagcctg gcaaaaacaa ggtcaagatt
    23041 tccagaatga actcggccaa aaacaaaaag acctggtcca ggaatatatg aaaaaaatcg
    23101 aagaagcgac cgcagccgtt gccgcacgcc atggcttcga tgtcgtcatt gataaaggaa
    23161 gcgaaaacac gctaaaaatt gtgctgtaca atagagatgg gttggatctc accaatgaag
    23221 tcgtcaaaga atttgaccgt cggtttaaat aacgaacgac taagagaaag cgtttgaaca
    23281 ttctcccctg tgggtcagac tcacagggga tttttgtatc tggagggtgg gctcaaagat
    23341 cagatgtgtt caaccgtcaa ggaatcacag aatgcgaaat tttctaaaaa tgctggaata
    23401 aagaatattg cttcagtgcg tcatacctcc aacagcctct tcctcagact gatagatggg
    23461 aatcacttgt ggcaaattag cacattcgag caactctctc acatgtgtct ggggactcac
    23521 gatactcaac cgaattcggt ccggcttcat ccgatggtac cagagaaaca tctgccccaa
    23581 cccgatcgag tcaatccagg taatattcga gaagttgaga ataatatggt ggcaccctaa
    23641 ctcctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaga tgcaacgaaa
    23761 aaaatccatg gagattcgga ggaagccaag tgtgtagaaa cccgggtagc tggctcctct
    23821 gcctaaatga aaatcaaaaa taagaatggt cttttttgca tcatttcaac tcaagggtga
    23881 tgatggtgtt cttgttaggg gggtagtccc tgacaagctc agagccctca cttgtgaaaa
    23941 tatgcacctt tccgccataa ctgttacccg gtatcgatac gctgacggtg ttgccggaaa
    24001 ttttagcgtg tatggtctta tgtttgttgg tatcgccggc gtccaccaga gtgaaaaagg
    24061 tgttcgcggt cgcgttttgc tgggggtaat agaatatatc cagatggggc gtggcttggc
    24121 tccaagtacg gtcattaccc tgatacatat tgcctttgac aatcaaactg ttggccttta
    24181 caaaaatcgg cacatgctct ctcaccacct cgacctcgag ccattgttta cccgaatgaa
    24241 tagagccgtt gtagtaatcg atccagctgc cctccggcag atacaccgtt cggatgggtt
    24301 cttgatcatg aatgggcgcg acgagtaacg aggggccaaa gaggtattga tcccagatgg
    24361 cataggtgtg ctcgtcatcg gggtacacgc ccgcaagcgg tttcatcagg gttccctgct
    24421 ccgcggcggt attcagcagt gagtaaatat agggaatcaa cgccatgcgc tctttgaaaa
    24481 cctgacggta caggtcctga aatgactccg agtaacgcca gggcgcccga tcgttgccgc
    24541 gaccactggc gccgtccagc ttaatctcaa aaaaccctgt ccatagacca aattgaatcc
    24601 aacgcagata aaggtcttca ctgatgaggc ccgtattgcc ggtgaaccct ccggcatctg
    24661 tcccccaatt gggaaaaccc aggtaactgg cgcgaatgga gttggccata ttgctgcgta
    24721 aaccttccca atttgtcatg acatcccctg cccagatggc actcaaatgg ggctgcgtgc
    24781 catgtaccgc cgcacgagcg aaattaaact ggtcttctcc ccagtaatcc gccagcgcct
    24841 catgggtatg tttggcatag agccaaataa atttgctgcg gcgctccaga atcggcgtcc
    24901 catcctgcca gctttcgtca tagggaaagc tctcatccgc ccgatccatt ttgtgcccct
    24961 gcacaccaac gttgtactgc aactccgtta agcgccgcgt aaattcgctg acagccttgg
    25021 ggttgctgag atcaaaatat cctttatgcc ctgccatgcg ccctgggaaa ttctgttcac
    25081 caaaagtcat cggcgcaatc caggtcatca aatggatgtg gtaatcctca tgtaagacct
    25141 ttatccactc ccgggggttg gagaaatcac gaccgaaatt catgtccgac caaccatgtg
    25201 ccccatcgct ataggggcga tcgacaaata atcccgtgaa gggaattttc atttcagtga
    25261 agcgtctggt gtcatcaaga acatcctgct tgcctcgctt gtggacatca cgccagatca
    25321 cggggccggt accccagagc ggaacatgtt tcggggcgcc aatgagctgg taataattaa
    25381 ccagcgcatg atccaaggtt ggtgcatcca gaacatgcca attaaactct ctggcggcgt
    25441 ggtacaactc ggtccggcca ttcatcgcaa attgataacg gccctcagcg aacgtgttga
    25501 aaaagctcac ataccctttc gtactaaaga acatgggtga aaccgcggtg gcatagtcgg
    25561 aactaaaccg gttgtgttct ccaatcgcat tgacatcttg taccaaaccg cgcaaatccg
    25621 ggcttttttg attatacggc tcggtagttt gtttcacgcc aaacatatgt ccgccgagat
    25681 cttccataaa aatgttgaca tgggaaaacc agtcagcatc actggagaaa tgcagccctg
    25741 cctgatcggc ttcaatatca atgatgactc tggaatcctc catggattcg atctcttcct
    25801 cgttcgccac atcacggtac gtcaggtgca aacgatagaa actgttactt ttcctgttga
    25861 gctggacatc atcccaggag tggaaatcca gcatgatatc ctgaatcacc atgatgggtt
    25921 gttgaccccg aaagaccacc aatgcgttct tcttttgctc aatcgttaac gggatagggt
    25981 cggcagcaca tgccaggttg ccaagcaata acgcaaccag gaaaatttga aggaatttca
    26041 acattcctct tctgtaaaac ttcgtccatc ccatccaagg cattctcggt ttccaattgc
    26101 agcaacctta acgacttggc tttaaaaccc aaaatc
//
LOCUS       DAORAQ010000027        26070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_362_length_26070_cov_59.371132, whole genome shotgun sequence.
ACCESSION   DAORAQ010000027 DAORAQ010000000
VERSION     DAORAQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 26070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26070
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_26070_cov_59.371132"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKM72_04375"
     CDS             <1..221
                     /locus_tag="PKM72_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60053.1"
                     /translation="ALEYLNKSKGWEIGVGPSLVEVHAGFAGALTPITAKEDLYAFFF
                     SIGRDSWQASGFSGPKLCRSFRRVVGNY"
     gene            complement(688..1131)
                     /locus_tag="PKM72_04380"
     CDS             complement(688..1131)
                     /locus_tag="PKM72_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNP60054.1"
                     /translation="MSEKIRHGSQLADGVPVRGWRGAFPPAQWLAVYRPQWLTHDAIA
                     GVTLAAYGIPVSLAYASLAGQPPQYGIYCNLVGGLFFALFGSSRQLAIGPTSAISMLV
                     GVTVADMAQGDPGPWADIAALTALVMAGMCVAPIIRIPEISQQIP"
     gene            1621..1944
                     /locus_tag="PKM72_04385"
     CDS             1621..1944
                     /locus_tag="PKM72_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60055.1"
                     /translation="MNLNDVVRKEFLVSSYESTLKLQITKFRKAGDELAVPKILDSMV
                     LMNRALSSVFSLQAGSHSNVTGHSFCSCMRLLMPSRTIHLGLSSDVDCDWKGVPQTLG
                     RACSM"
     gene            2045..>2793
                     /locus_tag="PKM72_04390"
     CDS             2045..>2793
                     /locus_tag="PKM72_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60056.1"
                     /translation="MHFPVQALHILKGGVMVGMLLSFPMTSLGMTKAITTPPPVSGHE
                     LSIESITPADVFARVQLVRKELDDIRFEMGQPKSREVGLLVENATPHEVFFQAKTLNQ
                     KVARLVTELTEEPDRIGTEEDPDDIRPFHVWKMVDTSLHRILSLKQQLGLPLTNSEAL
                     ADAETSPNQVFFVIGLANTQLNLLLMHQFSPRDAAELIRLGIEYTTHLLGQFPNAPAS
                     APLPPLERGRKSWNSYDHLIDCYMVLGSIARA"
     assembly_gap    2794..2893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2894..3089
                     /locus_tag="PKM72_04395"
     CDS             <2894..3089
                     /locus_tag="PKM72_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60057.1"
                     /translation="PRNSGNLPQMWVSMLAPVLIGRSRKPNKLPTMPLRYNRRILTCT
                     YSRMPHYRSSPYAQQLEQRF"
     gene            complement(3371..4408)
                     /locus_tag="PKM72_04400"
     CDS             complement(3371..4408)
                     /locus_tag="PKM72_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60058.1"
                     /translation="MDKVEKRIRAMITSIFSVTFFFMAGMGTGLASNVENPNEPNPIS
                     GHAISLEEITSADVMARVQMFLDTLELIRFEMGKPLVTPSDAIVTNVHSHEAYFQALT
                     LYHKADRLALELTGSTGVPPKPISLSALAPLHIWKMVNAGYIRILTIKEELGLKEIIP
                     ERPEDSATSLTEIGRAIVQANRQINLLQERHFSPSDVSQQVLLASQYTNRLLDRFPPS
                     TVTLETSPLVRGKQPADVFLRLVECYAILEKIAQQSRVPVLHLDLPAAHNVGISRQLY
                     PSDVYDMATLLVSDLAFFYTQLKKHDSLKPIPYPGRTFPSLVYQQATVLLHQLKSLEQ
                     LVTNDPKWLSR"
     gene            complement(4472..5101)
                     /locus_tag="PKM72_04405"
     CDS             complement(4472..5101)
                     /locus_tag="PKM72_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60059.1"
                     /translation="MITQFLERETLPYLGRVCLLLFLFGLGGCSEKAYGDSPNGELVD
                     LTGGVIPLLTYDSGGSFTGGGELFSGGSLSSRGDVRYLVRVKNQTGDPIDADSLILVV
                     DKIQEMARLRDVTTSLDIRGTDGNTEDGKPYFRVPVDGQAELPPYGESESFPIEIKNP
                     DLLRLYPPVLRVRGIRKTATHAFQGALQNLIQEGVVTPEEAEKAIDQSQ"
     gene            complement(5709..6050)
                     /locus_tag="PKM72_04410"
     CDS             complement(5709..6050)
                     /locus_tag="PKM72_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60060.1"
                     /translation="MNMQVICKLLFAATIVALIVGCASSPPRELVQHNDHTRLAAWYQ
                     KEARDLHARTEEMRQIKKEYEFLGTPKEGHESILVQHAKNLEHHYSKAAELAEAMAKA
                     HAEQAKNLQER"
     gene            7134..8648
                     /gene="ilvA"
                     /locus_tag="PKM72_04415"
     CDS             7134..8648
                     /gene="ilvA"
                     /locus_tag="PKM72_04415"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNP60061.1"
                     /translation="MPESYLKRILDARIYDLAIETPLDDTSLVSERMGNHVLLKREDL
                     QPVFSFKIRGAYNKLLHLTDEQRAGGVITASAGNHAQGLALAARHMGVNATIVMPRTT
                     PAIKVDAVKRHGGKVALFGDTYDEASTHAQSLLKEKGLTYIPPYDDPDVIAGQGTVAM
                     ELLRQHPAHIDAVFIPVGGGGLCAGMTAYIKCVRPEIKVFAVEPEDAACLKMAMEAKR
                     RVMLPQVGIFADGVAVARIGKEPFRVLRKTIDGVITANADEMCAAIKDIFEDTRSIAE
                     PAGALSLAGLKKYVAQTGCKGKTLIAIDSGANINFDRLRYISEQTEIGEGREAILAVT
                     IPEKPGSYMKFCSLMGKHSITEFNYRFSDNLDAHIFVGVQISPDKNDREDIISLLKGK
                     GYQVVDMTDNEVAKLHIRHMVGGHAPQAKNEIVFRFEFPERPGALLNFLTKLASRWNI
                     SMFHYRNHGSAYGRVLVGMQVPKPERKQLDILLNALGYPYRDETKNEAYQYFLG"
     gene            8860..10098
                     /locus_tag="PKM72_04420"
     CDS             8860..10098
                     /locus_tag="PKM72_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNP60062.1"
                     /translation="MSIYLVCKSERSPEGDSANSVFWGFLTLLCIPFVVVGIWVIFSP
                     HIPIRIFLLLSLTSFFPLGFFLGWELIVKNLVAGESWVNLIVLPVCSGLLTLVFVELF
                     FGSDLRKPDPVQNISAMNSPDRSYWYYARYRENGIERANSYGFVGPEPNPQIQDERVL
                     LIGDSIPAAERHPKNFPTISQEKFNKEFHQEEKLEIINASIAAYSVEQIYLYYLEKLR
                     DLPHDYLIFSFYLDDVNRNLRYRKNNRLYSPDWAEWQQDVYWQCYLCRRLLSLCNVSD
                     HLFLNYRKRTYEEAFPEALRVLEKTRLLANSRGAKFAVLNIPRFTWEGVLTNVDTYQY
                     FEMNKALERWCIGNGVPCYDTLPLLIGKEISTLRRSKTDIHFTYLGHQVVGLALKDFL
                     ASLIRPGKTIRGPGESVSGM"
     gene            complement(10336..10488)
                     /locus_tag="PKM72_04425"
     CDS             complement(10336..10488)
                     /locus_tag="PKM72_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60063.1"
                     /translation="MVESLLHGLEFTLLLLAMIAIQLHPAQGQRFYNRQQRLSYSKRV
                     TATPTE"
     gene            complement(10564..12063)
                     /locus_tag="PKM72_04430"
     CDS             complement(10564..12063)
                     /locus_tag="PKM72_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNP60064.1"
                     /translation="MALSLFWILLAPPFSHGGEPNKQPTPPIDDPFLLWQLDAVRERA
                     REIPQNIQERIRNGQEWNWNEYPDQVLLQEEAEIHWNRYLSAALNAPKWLDLGMTARL
                     RREGFDYPFKTDQKGSTWQWATRSRFRASARWKAFRAQLEFQGSTNGVDSATDVVGTS
                     TLTAGNVQQLFVALTLPDVWRTGLRTDLHVGRINLDIGSRRLVARSRFSNSSQAFDGI
                     HWNLANEGQWQFRTFFSEVVSNTRAADRLGLFTNSGNLFWGLSYETYQLSWSRIHLYY
                     FGRDEDAEGEQVARSHSTFGVRLYQPPRIGSFDYDGETAWQVGTLDGQDHFAYFQHFS
                     LGYTFALPWAPRILAMYDYASGTDSANGNYNQTFDNLFGARRSELTPTSLFGPFYRSN
                     ISSPGIRLILNPLPVLRLDLKFRAWYLAQSRDAWVNSGLQDPSGAAGNVLGQDLEVGV
                     RWNPSPNLSIDAGYDHFFKGSYITNQTNVPGNPPAQNTNYFYIQTEVRF"
     gene            complement(12210..13256)
                     /locus_tag="PKM72_04435"
     CDS             complement(12210..13256)
                     /locus_tag="PKM72_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60065.1"
                     /translation="MLLRLTASGETAYRPLFLSLVILILFFAPPTFAEWKVTGESNVY
                     YTDDAALFSATRRLSRSQDPTQPVIDSLLANQGDDVVYEPMAHITKSFPIMGRSTQLG
                     VRGQGFIFTNNTQFNHGSLSVEASHHLTSSTHLLMRYFFGPDLYLGKNEVRPLEETGG
                     NTPKVKKEEVTTNYWAGGLAQQIPGMDDARIILYGRYGLREYDKPFTQRDTRFWTIGT
                     HVEWKPAQKIGLALGYHYERGLSDGRKQPELQDDTSYFNHFVTGEMDLEITERFGLEM
                     ALHYEFNGWTTGIEGDERKGQHENVVQGDIAVRYQVNELLQLTGGFQGAHRKESFEKG
                     LKNLNTWVGAKWAF"
     gene            14135..14671
                     /locus_tag="PKM72_04440"
     CDS             14135..14671
                     /locus_tag="PKM72_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNP60066.1"
                     /translation="MGLFVLLSLPAVFGCSSISVSSDYRESTDFSELKTFSWMLKTPE
                     GEIDLGGANHMARQRIESAIAAELANKGYIEIVAGKSDFHVTYYVGREERIQVQSMGG
                     PLMRPYWGMGMGYAEVYQYEEGTLIIDLLDAKAPQNIIWRGIAKGVMDWKGTTGGQTG
                     LINEAVQKVFAQFPPKRS"
     gene            complement(14847..15587)
                     /locus_tag="PKM72_04445"
     CDS             complement(14847..15587)
                     /locus_tag="PKM72_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNP60067.1"
                     /translation="MGNTEPFASLLKGNIKGALADGEKAILKIVMVTHAGMTRDKFEK
                     TVNDWIATAKHPQTGRLISEMVYQSMMELRAYLRAHEFTTFIFSGGGIEFMRPWTEHV
                     YEIPPEQVIGSSGKLKYALRDGYPVLLKLPEINFIDDKDGKPVGIQTHIGRRPILAFG
                     NSDGDFQMLQWAASGSGASFMALIHHTDPERERAYDRKSSIGHLDKALDEAKGKGWAV
                     VDMRQDWRTIYPFHKHGTDSPMSESSGK"
     gene            complement(16075..16320)
                     /locus_tag="PKM72_04450"
     CDS             complement(16075..16320)
                     /locus_tag="PKM72_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60068.1"
                     /translation="MQLRSLFTTTAILEAGTGLALFLSPPAPVAVLVGTSLGSDGGLI
                     TACSAGAALFSLAVACWFARNDGQSRSATGLVMAMLL"
     gene            complement(16581..>18857)
                     /locus_tag="PKM72_04455"
     CDS             complement(16581..>18857)
                     /locus_tag="PKM72_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNP60069.1"
                     /translation="PPTPSASIAGRTMQESIYRQRVEPKRLPADVPNILLVLIDDSGP
                     GLPATYGGEVHTPTLDRIAKQGISYNRFHTTAMCSPTRASLLTGRNHHRVGAGQIAEL
                     ANDWDGYSGLQPKSSAMIPDVLKSYGYHTGAWGKWHNTPAEQTTAAGPFDYWPTGYGF
                     EYFYGFLAGEASQYEPHMVRNTTVVQPTHKPGQPYHLTEDIAHDAIGWLRQHKAFTPD
                     KPFFMYWAPGAIHGPHHVAKEWADKYKGKFDDGWDRYRERVFLRAKEMGWIPQNAQLT
                     PRPETLAAWDSIPEDEKPFQRRLMEVAAGFAEHTDVQVGKVVDEIDRLGYGENTLIFY
                     IWGDNGSSAEGQNGTISELLAQNGIPTKISQHIEALDALGGLDVLGSPKTDNMYHAGW
                     AWAGSTPYKSTKLVAAHFGGTRNPMALRWPAKIKHSATPRAQFHHVNDVVPTIYEILG
                     ITPPRMVNGIPQDPIDGTSFAYTLNDEKAQGRLHTQYFEVMGSRAIYYDGWMASAIGP
                     RIPWVPGLPKGIQDWTPDKDRWELYNIEEDWTQANDLADSMPEKLAQMKELFAMEAAK
                     NGALPIGGGLWVVALHPELRKSTPYKEWTFSGDIIRMPEFTAPALGNKPNTVTIDTEI
                     SANADGVLYALGGFSGGLALYMEDGTLSYEYNLFEIHRTQIKAKEKLSAGKVKIEVLT
                     SYVEPKPAGPLKIILKVNGKEVASGIVPISAPLLFTANDCLDIGTDLGSPVSLDYYDK
                     APFKFDGKIETVHVQYTE"
     assembly_gap    18858..18957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18958..20477)
                     /locus_tag="PKM72_04460"
     CDS             complement(<18958..20477)
                     /locus_tag="PKM72_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNP60070.1"
                     /translation="MNSIRRILFGLLAIFIAPSLAMAGGKPNILVIWGDDIGGFNISA
                     YNMGMMGYQTPNIDRIAKEGALFTDWYGQQSCTAGRAAFITGQSPMRTGLTKVGLPGA
                     PEGMKKEDPTIATLLKAKGYMTGQFGKNHLGDRDEMLPTNHGFDEFFGNLYHLNAEEE
                     PEHPDYPKNPEFKKRFGPRGVIHSFADGRITDTGPLTKKRMETIDEEVNVKALDFMER
                     ARKEDKPFFLWWNSTRMHIFTHLKEESVGKTGLGTYADGMVEHDGHVGQVLDKLKELG
                     LEENTIVMYSTDNGSESFSWPDGGTTMFRGEKNTQWEGGYRVPTAIKWPGVIKPGTVI
                     NDIAAHEDMLPTLLAAAGDTTVKEDLLKGRQIGDMTYKVHIDGYNLLPALKGEGEWPR
                     KEFIYWTDDGNVAALRYGNWKATFLRQDADGLRVWQEPFVKLRAPMLTNLRMDPFEHA
                     HEIGMDYDRWFAEHMFMFAPAAGYVGKWLQSFREFPPRQKPGSFNLDNVMEAVTSGT"
     gene            complement(20886..21383)
                     /locus_tag="PKM72_04465"
     CDS             complement(20886..21383)
                     /locus_tag="PKM72_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60071.1"
                     /translation="MGMVSTVKHEVKEVGLVTLYFFFCFGIVLTLMKLMLEAYEIEFY
                     AFSKSLVGALIVAKVVVLLGKTRAGTRFDAHHSLGAAALYRTLVYSGCTFLVLFAEKM
                     FHAFRESGLLGAAFMAVWEQKDFSLIFAKVICIGLAFMGYFLYRGIERRLGEGTLRRI
                     VFGPP"
     gene            21661..21915
                     /locus_tag="PKM72_04470"
     CDS             21661..21915
                     /locus_tag="PKM72_04470"
                     /inference="COORDINATES: protein motif:HMM:NF028504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5132 domain-containing protein"
                     /protein_id="HNP60072.1"
                     /translation="MEAVIGGVVVGAVVAVAVPSIASGVGSVVRPIAKGMIKGGLVAY
                     AAVSELVSETGEQFNDMIAEAKAEIGNNDKKMTRAKSQRA"
     assembly_gap    22406..22505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22623..23204)
                     /locus_tag="PKM72_04475"
     CDS             complement(22623..23204)
                     /locus_tag="PKM72_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60073.1"
                     /translation="MDCVKRPTLFLLIGLTGIWFIMTGCTSVDTIRFTSQTFPPKKSV
                     LDVEVMALEPKCPHIAIAQLSVLDSAVSYEDEEGAILKKAADLGADGVVLHRGTKRTV
                     ATPAYGGYAYNYSYPGWGYSSYGMVGYGGYGTTGYTIGGYGYSPYGMGTPMMYDTTVR
                     SLTGIAIRYQDGPRCVTGGPESRQISISLPLQS"
     gene            complement(23220..23687)
                     /locus_tag="PKM72_04480"
     CDS             complement(23220..23687)
                     /locus_tag="PKM72_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60074.1"
                     /translation="MTNIFALVLTLTMGIIGSSLAANEEMHPLPSILSDIERQIAELT
                     INIEKISDRIKYLHEIPDSKDPLIQEVRNLDLRGWELHQEQWKVQRDGLRFTEKLLRK
                     IQDHPDEKPEALQTWLGRLKEFKETMNDYRQQRAGIEVLRIESEVKLIEQYLR"
     gene            complement(24056..24727)
                     /locus_tag="PKM72_04485"
     CDS             complement(24056..24727)
                     /locus_tag="PKM72_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60075.1"
                     /translation="MITSWLLGLTLGMTHALDPDHLIAMGTLAAESRDIRRSVLLGVI
                     WGVGHTCALALVGFLVLSLKWTIPIHMAANMEIMVGAMIVALGVHLLWRTLQPWTVHL
                     HEHHHKEMTHSHVHIHGQDHGSHSHHLGGSRAKVLLVGFVHGMAGSAALTLAVLTTIP
                     SMAMGMIYILIFGVGSIGGMLLMSGLISLPFVFVSQSWHHNLKVSAGCLAILFGAYFI
                     WSPFS"
     gene            complement(25180..>26070)
                     /locus_tag="PKM72_04490"
     CDS             complement(25180..>26070)
                     /locus_tag="PKM72_04490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNP60076.1"
                     /translation="TKSAILQFVAKVTEEGSPDYVPSHDRIAVFDNDGTLWAEQPMYF
                     QAIFALDRVKALAPQHPEWKTQEPFASILKGNVKEALAGGEKALLELVLATHAGMTTE
                     DFRKTVTEWIATAKHPKTKRLYTEMVYQPMLELLDYLRSNGFKTFIVSGGGIEFMRPW
                     VERIYGIPPEQVIGSSIKTQYEVREGKPVLVRLREFNFFNDKAGKPVAINQYIGRRPL
                     MSFGNSDGDFQMLEWTTTGEGPRFGLLVHHTDDEREWAYDRESHIGRLSRGLDEGAKR
                     GWTIVNMKADWKRVFSFEQP"
BASE COUNT         6185 a         6349 c         6610 g         6626 t
ORIGIN      
        1 aagcccttga gtatctcaac aagagtaaag gttgggagat cggcgtaggt ccgagtctcg
       61 tggaggtgca cgccgggttt gcaggggctc tcacccctat aacggccaag gaagatcttt
      121 acgctttctt tttttctatc gggagggact catggcaggc atcgggcttt agtgggccaa
      181 aattatgcag atcattccgt cgtgtggtgg ggaactatta attttatgag acgtggtggt
      241 cggatgactt ctcctccttg ccacggacta atctatcgaa ggttttttca tactcggatc
      301 tacgacttga gcctacaccc gaggaaattt ttttaggtaa tgataatcgt tctttcgatc
      361 gagaggagct ttttttgagg ggaaaatttg gcgaaaaagt aaacgtagag aaatgtttcg
      421 tgaaaggttt atggacaggt tgtgggtggt cttaggcgac taacatccaa atgaggtatt
      481 cgagtatctc cgatttttct ttccggtaaa tccaatcagg tgctagagat ccggttttac
      541 attttataat tctgttccca agtcaacacc ggctggttga cggtcgcatc ccttgtctca
      601 atctgccgtc ttacttctgt gacagggtac tgcccactga aatccctccc actactttct
      661 tattcgattc gaatacctgg tcatacgtca agggatttgt tggcttatct ctggaattct
      721 aatgattggg gccacacaca tgcctgccat aaccagtgcg gtcagtgccg cgatatccgc
      781 ccaaggtccc gggtcgcctt gtgccatgtc ggcaactgtg actccaacca gcatggagat
      841 cgccgaggtt ggcccgatcg caagctgccg cgacgaaccg aacagtgcga aaaacaggcc
      901 cccaaccaga ttgcagtaaa tgccgtattg tggcggttga ccggcgagcg aggcataggc
      961 aagtgagacc ggaatgccgt aggcagcgag cgtcactcct gcaatggcgt cgtgcgtcaa
     1021 ccactgtggt cggtagacag ccagccactg tgcgggggga aacgccccac gccatccgcg
     1081 gaccggaacg ccgtccgcga gttggctgcc gtgtcggatt ttttcactca taatgtcgca
     1141 tcccgtaaac catcttctct ggcatccacc gaccgtttcg gaaaattgag cgggagttta
     1201 aatttggagt tagaccatac atggtcgacg atactttcgg cccgggagca ctggagggaa
     1261 actgtaccat aatttcctga aaaacttgac catgactttt taaaatatgg tcatccggga
     1321 acatgatacg aacgcgggga atcttgctgc ccaaagaggt cgggcgatgg attcgccgga
     1381 cttgaacgcc tctcctgtcc tctgtgagat cggccccttt cgcatgggag ataccgatag
     1441 gaccaacggg cactcgcctg tcacagggca caggaggccg gattggatac agatttgccg
     1501 ttcagcttga tcgaagaaat tttcgggtgt tgtatctggc ggaggaggcc ttcaccatgt
     1561 ttttctctct gtgtatgaga aatgtggagg tcaaccattg gtggcgacat tgaaacacca
     1621 atgaatttaa atgatgtggt gaggaaagag tttttggtat cctcctatga aagtaccttg
     1681 aaattacaga ttacgaaatt ccggaaagcc ggggacgaat tggctgtacc gaaaatttta
     1741 gactctatgg tgctcatgaa tcgtgcgctt tcatcggttt tttccctcca agctggttcg
     1801 cattccaatg ttacagggca ctcgttttgt tcgtgtatgc ggttattaat gcccagtcga
     1861 acgattcact tggggctatc ctcagatgtg gattgtgatt ggaagggggt gccgcaaaca
     1921 ttgggaagag cctgttcaat gtaaaatagt cgtttaagaa aaataatatg gacaaccgca
     1981 gcccagcgaa tccctcagtc aggctcgacg ggagagatgt gggcttttac aaaaggcgat
     2041 aacgatgcat tttccagttc aggcgttaca cattctcaaa ggcggagtga tggttggaat
     2101 gttacttagt tttccaatga caagtcttgg aatgaccaaa gccatcacaa cccctcctcc
     2161 cgtgagtggt catgaacttt caattgaatc catcaccccg gctgatgtgt tcgcacgagt
     2221 tcaactggta cggaaagaac tggatgacat ccgttttgaa atggggcaac ccaagagtcg
     2281 ggaagtaggt ctcctggttg aaaatgcgac tccccatgag gtgttctttc aagccaaaac
     2341 ccttaatcaa aaagtggcca ggttggtaac ggaactcacc gaagaaccgg atcgcattgg
     2401 aacagaagaa gatcctgacg atatacgtcc atttcatgtc tggaaaatgg tcgatacctc
     2461 tctacatcga atcctgtctc ttaagcagca actcgggctc cccctgacta atagtgaagc
     2521 tttggccgat gccgaaacgt cccccaatca agtctttttt gttattggat tggcgaatac
     2581 ccaactcaat cttttactga tgcatcaatt ttctccgagg gatgccgcag aattaatccg
     2641 cctgggaatt gaatacacca cgcacctctt gggtcaattt cctaatgctc ccgcgtctgc
     2701 tcctctccca ccgcttgaac gaggtcgcaa gtcatggaat agctatgatc atctcattga
     2761 ttgctatatg gtgttaggaa gcatcgccag ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnntcctcgt aattccggga atctgcctca aatgtgggtt tcaatgctag
     2941 ccccggttct cattggccga tcgcgcaagc ccaacaagct tcccacgatg ccacttcgat
     3001 acaaccggcg aattctgact tgtacgtatt ccagaatgcc gcattatcgt tcgtcaccat
     3061 atgcgcaaca attggagcaa cggttttagg catgtccatg ataccgggag ccggagcctg
     3121 atggtaaaat actgatcaag cagaccgaga ttctgttgtg gatcagtaga gtggtatgac
     3181 atgcgatatg ggtaattttt ccactctgct gaaaaacacg gctgccgtcg tcgtacctca
     3241 gatgggttag tagatggccc aggcttttgc ccgggaatgg tgctgcatgc tgggctgcgg
     3301 gtgtggtgtt aggtctgcgt ttgtaacaag aaggtgatgg tagaaatcgt ttagagaaaa
     3361 tatttcttct ctatctggaa agccattttg gatcgtttgt tactaactgt tccagtgatt
     3421 tcaactgatg aagaaggacg gtagcttgct ggtaaaccag ggaagggaag gttcgtcccg
     3481 gatagggaat gggcttaaga gagtcgtgtt tttttagttg tgtataaaaa aaggcgagat
     3541 ccgaaaccag gagggtagcc atatcataga catcgcttgg gtataattgc cgcgaaattc
     3601 ccacgttatg cgcggcaggc agatcaaggt gaagaaccgg aacgcgggac tgttgcgcaa
     3661 tcttttccag aatggcatag cattcaacca agcgaagaaa tacatcggcc ggctgtttgc
     3721 ccctcactaa aggggaagtc tctaaagtga cggttgaggg gggaaaccgg tccagcaacc
     3781 ggttagtgta ctgactggcc agaagcactt gttgcgatac atcactgggt gagaaatggc
     3841 gctcctgcag taggttgatt tgccggttgg cttgtacaat ggcgcgtccg atttctgtca
     3901 gacttgtcgc ggaatcctcg ggtcgttccg ggataatttc cttcagacct aactcctcct
     3961 tgatggtcag aatacgaatg tagcccgcat tcaccatttt ccagatatgt aaaggcgcaa
     4021 gagcagaaag cgaaatgggc ttcggtggaa ctcctgtgct cccggtgagt tccaaagcga
     4081 gacgatcagc tttatgatag agggtcagag cttggaagta ggcttcatgg gagtggacat
     4141 tggtgacgat ggcatcggac ggggttacca gtggttttcc catttcaaac cggatcaatt
     4201 ccagggtatc cagaaacatc tgaacacggg ccatgacatc tgcagacgtg atttcttcga
     4261 gagaaatggc atgcccgctt atggggtttg gttcattcgg gttttctacg tttgaagcca
     4321 gccccgttcc cattcctgcc atgaagaaga acgtcacact gaatatgctg gttatcatgg
     4381 cccgaatacg tttttccact ttatccacta ttctcatccc ttctgaattc atgcggaatt
     4441 tcaccgccca cgtatcttcc gaatgttatt tctattgtga ttggtctatg gccttttccg
     4501 cttcttcggg ggtcacgaca ccttcctgga tcaagttttg gagcgcacct tgaaacgcat
     4561 gagtggccgt ctttcggatt cctctcaccc gtaagaccgg agggtataag cgaagaaggt
     4621 ccgggttttt gatttcgatg gggaaggatt cactttcccc gtatgggggg agttctgcct
     4681 ggccatcaac aggaacccgg aaatacggtt tcccatcttc agtattccca tcggtccctc
     4741 ttatatcaag gctggtggtc acatcgcgga gccgtgccat ttcttgaatt ttgtcaacga
     4801 cgaggatcag tgagtcggca tcgatgggat caccggtctg atttttaact cgaaccagat
     4861 agcggacgtc gcctcgagag gaaagacttc cccccgaaaa taattctcct ccgcctgtaa
     4921 agcttcctcc ggaatcatag gtgagcaagg gaatgactcc tcccgtgaga tccacgagtt
     4981 cgccattcgg agaatcgcca taggcctttt cactgcaccc tcccaaacca aaaagaaaca
     5041 gcaggagaca gacccgccca agatacggaa gagtttccct ttccagaaat tgagttatca
     5101 tggcgtcatc ttacagagaa cgcgttgaac ctgcaaaggt atgcgtgttc atcaacttcg
     5161 ccatattcaa ctcccggttt ttgtgtagag aaaccgaaca gggccatatt cgaacataga
     5221 gtcggtttgg ttatagggca actcactcga tacctcgtat ccattgccca ggtcagggta
     5281 taggggaaac ggcgttcaaa gtgaatcttg taatgcaggg gactgtacaa atggaagaag
     5341 ctgactgcgc aatcggtcag cagccctgtg gaatttctca ttgaggtaac ttgatcctcc
     5401 gtctgaaggg tggatgcctt ggattgaaat tcaaacaggg gattttgaat aaccatcctg
     5461 atgccatcat cattgcaacc aaatccgagg gcataggacc aaatgttttc accactaccc
     5521 aaatgtctgt aaaaggcata ctaaccgttt atctggggtg caaagcttga ccctccggtc
     5581 tgggtacgaa tcgaaaaatt cagggcgtca aatttaataa acgtgttcca tgcctcatca
     5641 caggaggaat attgaacatg tgaaacgcag cgatcacctt tccctctaaa agacctgata
     5701 agttccgttt acctttcttg caggtttttt gcctgttcag catgggcctt cgccattgct
     5761 tcggcgagtt ccgcggcttt ggagtaatgg tgctctaaat ttttagcatg ttgcacgagg
     5821 attgattcat gtccttcttt gggagttcct aagaattcat actctttctt aatttgacgc
     5881 atctcttccg tccgtgcatg aagatcacga gcttctttct ggtaccaggc ggccaaacgg
     5941 gtatgatcgt tatgctggac caattctcta ggcgggctgg aggcgcaccc aacgatcagg
     6001 gcaacaattg tggcagcaaa caacaattta cagataactt gcatgttcat gatcccttcc
     6061 tttgtgataa atacatgtgg gtttttcggc tatttttgcc gtgcgataat tggtagacaa
     6121 ttagtttaag gtgaagaaaa aatacgacgg tttacaccct cagggcctcg tatatcgttg
     6181 gccccttcac tgtgcaggaa agaaagatgc gttgaatgcc atggatcctc tcatcagatc
     6241 catttatcgg tttccgtcct gttagacatg gagaccatcg cgtttcccaa aggcgacctt
     6301 ccatgattgg aatcttaacg tccacaggag aatatccgat gaagatccaa aaataggaag
     6361 tacgagatag gcagggttca gtcctgaacg tccgagtgtt tggccaaatt cctttgtttc
     6421 ttgatgtaat cctaacaaag tggcatgatt gaaaaaacct ttctcacccg aatgtgcaat
     6481 cgatgcggaa acgtccccag gtgttcatcg ttgcttttcc cttgggttga aacatcgcgt
     6541 tgaagtgatt ccgggactcg gcaatatttg aaaaaaattg gaaagacggt cctcgaaaaa
     6601 atcgggtatg acggcttcat acccttccgc cacaggcaga atacgtattc atcaagtggt
     6661 gcatttaatt tatctatttc ctggttcacc cctctgatgg aatcgtaggc atccatgaga
     6721 tatcgggcgt cagaatgcac caggtcctct actggccgtt tagtcgattg attaaagcgt
     6781 cagaggtaaa aacatttttg aagggtgaga ggcgaaggga tgtcccgatg aacgcggcac
     6841 ccgtaagata gaactctgtg acctctcttt tgtgtggaat gtccagccag gtcaagccca
     6901 tgatttgatg caggtcttgt gaatcctcca ggacagtctt catactccta tgatgcctaa
     6961 agcattggtc tgattcagac tcataccaaa ttggaagttg ctgtgctttt ggaaagggaa
     7021 ttatatatta tggttcgaga ggaactatca tgaactacag cccggagagt gccattgata
     7081 gaaatttcca tgagtgcgaa cggttgcaac gacagaatcc aatttagaaa cttatgccag
     7141 agtcttatct gaaacgcatt cttgatgccc gcatctatga tctcgccata gaaacaccct
     7201 tggatgacac ttccctcgtt tccgaacgca tgggtaatca tgtgttattg aaaagagaag
     7261 atttgcaacc tgtgttttcg ttcaaaattc gtggtgcgta caataaatta ttacacctca
     7321 ccgatgagca gcgggcgggt ggcgtcatta cggcctcggc gggcaatcac gcacagggtc
     7381 tggcgcttgc ggcgaggcac atgggtgtga atgccaccat cgtcatgccg cgaacaaccc
     7441 ccgcaattaa ggtggatgct gtcaaacgtc atggcgggaa agtcgcgttg tttggcgaca
     7501 catacgatga agcgtcaacc catgcgcaat ccttgttgaa agaaaaagga ctgacgtaca
     7561 ttccgcccta cgatgatccg gatgttatcg ctggtcaggg tactgttgcc atggagttat
     7621 tgcggcaaca tcccgcccac attgatgccg tttttattcc cgtaggcggg ggtgggttgt
     7681 gcgcaggcat gacggcctat attaaatgtg tgcgaccgga aatcaaagtg tttgccgttg
     7741 agccagaaga cgcggcctgc ttgaagatgg caatggaagc aaagcgacgg gtgatgttac
     7801 ctcaggtggg tatattcgcc gatggggttg ctgtggcacg aatcggcaag gagccgttcc
     7861 gtgtgctaag gaaaactatc gatggcgtca tcactgccaa cgccgatgaa atgtgtgccg
     7921 ccattaaaga tatctttgag gacacccgct cgattgccga gcccgccgga gcgctttcgc
     7981 tggcaggatt gaaaaaatat gtggcgcaga caggctgcaa gggcaaaaca ttaattgcga
     8041 ttgatagcgg agccaacatt aattttgacc gtttgcgcta tatcagcgaa caaacagaaa
     8101 tcggcgaggg tcgggaagca attctcgcag tcactatccc tgaaaagccc ggaagctata
     8161 tgaaattctg cagcttgatg ggaaaacatt ccattacaga atttaattac cgattttcgg
     8221 ataatcttga tgcacatatt tttgtcggtg tgcagatttc acccgataaa aatgatcgcg
     8281 aagatattat ttcgctctta aagggaaaag gttatcaggt ggttgacatg accgacaatg
     8341 aagtggctaa gctgcacatt cgccatatgg tgggagggca tgcgccacag gccaagaatg
     8401 aaatcgtatt tcgttttgaa tttcccgaac gaccgggagc tctcctgaat tttctcacca
     8461 agctcgcttc ccgctggaat atctccatgt ttcattatcg caatcacggt tctgcctatg
     8521 gccgtgtgct tgtgggtatg caggtgccta aacctgagcg caaacagctt gatattcttc
     8581 tgaacgccct gggttacccc taccgggatg aaacgaaaaa cgaagcctat caatatttct
     8641 taggctaaat cgatggcgag aagtttctcc aactccagga atattggggg aattttgaag
     8701 actagtcgat gggctgcatt tttaatttgt gccactattc ctatcctacc cctatgggca
     8761 tacttctctg atcaactcct acaagaatta atttctgtta ggagtcagag cacgtctcta
     8821 tggttgggtt actgtgtttc ctggttggcc tcagtgggaa tgtctatcta tctcgtttgc
     8881 aaaagtgaac gttctccaga aggggatagt gccaattccg ttttttgggg attccttact
     8941 ttactctgta ttccttttgt tgtggtcggg atatgggtta tttttagtcc gcacattccc
     9001 atccgaattt tcctgctcct ttctctgacc agctttttcc ccctcggctt ttttctggga
     9061 tgggagttaa ttgtcaaaaa cctggtcgct ggagaatcat gggttaacct tattgttctc
     9121 ccggtatgct ctgggctcct gacgcttgtt tttgttgaac tcttcttcgg gagtgattta
     9181 cggaagccag acccggttca aaatatttca gctatgaata gcccggatcg gtcctattgg
     9241 tattatgcac gatataggga aaatggaata gaaagggcaa attcctatgg gttcgtagga
     9301 cctgaaccaa atcctcagat acaggacgag agggttctcc ttattggcga ttccattccg
     9361 gctgccgagc gccaccctaa aaactttcct acaatctccc aagaaaaatt taacaaagaa
     9421 tttcaccagg aagaaaaact tgaaattatc aatgccagta ttgctgctta tagcgtggaa
     9481 cagatttacc tgtattattt agaaaaacta cgagacctcc cccatgatta tttgatcttt
     9541 agcttttatc ttgatgatgt taaccgaaac cttcgttacc gaaaaaacaa tcgcttatat
     9601 tctcctgatt gggctgagtg gcaacaggat gtctattggc aatgttatct ttgtcggcgt
     9661 ctcttgagtc tctgtaatgt gtcggatcac ctatttctta attatcggaa aagaacttac
     9721 gaggaagcct tcccggaggc attgagggtt cttgagaaaa cccgccttct ggccaattca
     9781 cgcggagcca aatttgccgt tttaaatatc cctcgtttta cgtgggaggg ggtacttacg
     9841 aatgttgaca cgtatcaata tttcgagatg aacaaggctt tggaacggtg gtgtataggc
     9901 aatggcgtgc cttgctacga taccttgccg ttgctgattg ggaaagaaat ttctaccctg
     9961 aggaggagca agacggacat tcattttact tatttgggac accaggtcgt tggtcttgcc
    10021 ctaaaggatt tcctggcttc actaatacgg cctggcaaaa ccataagggg tcctggtgag
    10081 tctgtatcgg gcatgtagat gagaagccgt cttgcgtagg aatcgaggct tcatcatttc
    10141 tacctccagc aacgaagagc cccatcgttg gctcagcttt caccgttcca accgtggtag
    10201 gggagggcaa aaaacgcgga cggcaaagaa tcgaaccgtt cattttgcca gggtaagtat
    10261 tgtccggccc ttctgcatca attgaaaagg gtttccgtcg tttgggtgga gacttaattt
    10321 aagtgtttgg actccctatt cggtaggtgt ggccgtaacc cttttcgagt aagagagccg
    10381 ttgctggcgg ttataaaacc gttgaccctg ggctggatga agttggatag caatcatggc
    10441 caataggagc agggtgaatt ccagcccatg tagcagtgat tcaaccatcc aaatgcacca
    10501 ggctcccttt tgctctccgg ccgtgatccc ttgccgttct cccagggtta tagcaccagg
    10561 tcatcaaaat ctgacttcgg tttgaatata gaaataattg gtattttggg ccggaggatt
    10621 tcccggcacg ttcgtttggt ttgtaatata ggagcctttg aaaaagtggt catagccggc
    10681 atctatgctg agattgggcg agggattcca ccgaacgcct acctccagat cctgtcccaa
    10741 cacgttgcct gcggcgccgg atggatcctg caggccggaa ttgacccacg catcccggga
    10801 ttgggccaaa taccaggccc ggaatttcag gtcgagtcga aggacgggaa gcgggttgag
    10861 aatcaatcga atgcccgggg agctgatgtt ggaacggtag aatggtccaa acagactggt
    10921 gggtgttaat tcactgcgcc gcgcgccaaa aagattatca aacgtttggt tatagtttcc
    10981 gtttgcacta tcggttccgc tggcgtaatc gtacatggcc aggatgcggg gagcccaggg
    11041 gagcgcaaag gtatagccca aggagaagtg ttgaaaatac gcaaagtgat cttgcccatc
    11101 cagcgtaccc acctgccagg ccgtttcacc atcataatca aatgatccga ttcgtggtgg
    11161 ctgataaagc ctcacgccaa aagtagaatg ggagcgcgcc acctgttccc cttcagcatc
    11221 ctcatccctg ccgaaataat acaggtgaat tctggaccag gagagctggt aggtttcata
    11281 ggaaaggccc cagaacaaat tcccgctatt ggtaaacagg cccagacgat ccgcggcacg
    11341 ggtgtttgaa accacttcag aaaaaaaagt ccggaattgc cactgacctt cattggcaag
    11401 attccagtga atgccgtcaa aggcctgaga ggaattgcta aaacggctgc gggcaacaag
    11461 acggcgcgac ccgatatcca ggttgatgcg cccgacatga agatcggttc gaagtccggt
    11521 ccgccacaca tccggcaagg tgagcgccac aaagagttgc tgcacgttgc ctgccgtcaa
    11581 tgtcgaggtc cccactacgt cagtggcgga atcgacaccg tttgtggacc cctgaaattc
    11641 cagctgagcc cggaaggcct tccaccgtgc gctggcccgg aaacgggatc tggtcgccca
    11701 ttgccaggtg cttcccttct ggtccgtttt aaagggataa tcaaagccct cgcgccgcag
    11761 acgagcggtc atgccgaggt ccaaccattt cggcgcattg agggccgcag acaggtaccg
    11821 gttccaatgt atttctgcct cctcctgcaa gagcacctga tcgggatatt cgttccaatt
    11881 ccactcctga ccgtttcgaa tccgttcctg gatattttga ggaatttctc tggctctttc
    11941 cctgaccgca tccaattgcc aaagcaggaa aggatcatct ataggaggag ttggttgttt
    12001 gttgggctct ccgccatgcg aaaagggtgg agccagcagg atccaaaaca aggaaagagc
    12061 catgaccttt ccggcattcc tgctgtgggt gaaccatatc cgcatggaag ctaaactatg
    12121 gacgaagctt ggactgaggc tggcttaaca tttgtaatgt gccgattccc tggtttcctc
    12181 ggaggattat ggatttcgct acgaaaaaat taaaatgccc atttggcgcc tacccaggta
    12241 ttgaggtttt ttaacccctt ctcaaaactt tcttttcgat gcgctccttg aaagccaccc
    12301 gtcaattgaa gaagctcgtt tacctgatat cgaacggcaa tatccccttg caccacattt
    12361 tcatgttgtc ccttgcgttc atccccctca atgcctgtgg tccatccgtt aaattcataa
    12421 tggagcgcca tttccagtcc aaaccgttcc gtaatttcca ggtccatctc tccggtcacg
    12481 aaatgattaa agtaggacgt atcatcttgt aattctggct gcttccgtcc atccgacaag
    12541 ccccgctcat aatggtagcc cagagccagc ccgattttct gtgcgggttt ccattccacg
    12601 tgcgtcccga tagtccaaaa ccgggtatcc cgttgtgtga agggtttatc gtattcccgc
    12661 aatccatagc ggccatagag gataatcctg gcatcgtcca taccggggat ctgttgggca
    12721 agtcctcctg cccaataatt ggtagtgacc tcttcctttt ttacttttgg tgtatttccg
    12781 ccagtctctt caagtggccg cacctcattc tttcctaaat atagatcggg tccgaaaaaa
    12841 tagcgcatga ggaggtgtgt ggaggaggtc agatgatggg aggcctctac actcagggat
    12901 ccgtgattaa attgagtatt gttcgtaaag ataaaccctt gtccgcggac acctaattgg
    12961 gtagatcgac ccattatcgg gaaggacttt gtaatatgag ccatgggttc atacaccaca
    13021 tcgtcccctt ggttagcgag aaggctatca ataaccggtt gagtagggtc ctggcttcgg
    13081 ctcagcctcc gggttgccga gaacagggcg gcatcgtcgg tataataaac attgctctca
    13141 cccgtgacct tccactcggc aaacgtcggg ggcgcaaaga atagaatgag gatgacaaga
    13201 gataaaaaga gcggcctgta agcagtctca cctgaggcgg ttagtctcaa aagcataggt
    13261 tgtcgggttt tcatttgtcg gcggacatct atcttttttg catcatacct gcatgactgt
    13321 ggaaggtatc tatctgaaca cacacacaga tgaattctcg atactcaatg tcgggaatga
    13381 ctgctgtgga agggttcccg tttgcacgtg ttgggaatgt cctgggtgga aagatttccg
    13441 atttaaccat acaggaataa cgaggttaaa ggtgcaaggt tggcacgaaa aaaattaaaa
    13501 gtgggggttc actttcagat tttttcgtat gctcaccctt catcaggcat agtccgtggg
    13561 ttcatggtga cctgtttgcc attcagaccc aacatgccct cggtcgcaga agattgggtt
    13621 ggacgacatg ttctttaacg tatctatcca taacgatgca atcaccatcc cacccctcat
    13681 ccgccttcct tgaggaggta cagaggatct gaaggtcgcg agtcgaaaag ggtgagaagt
    13741 gtctgttata gtctgttgcc gcgaaaccgg attaaccaga aagcatgccg gacgaccacc
    13801 catttacact catgatcgtc gcggtggggg cggagcactt ctggtgtcca ttcgaattgc
    13861 ctattttaag cagattactg ttctcccggc agaatttgcc gtctcacaag ggagaacatt
    13921 gagcttcaat gaagcgggaa aattcaataa ccactgtggc tttcaccaag cagagggcgc
    13981 aaattgctca cagatttgcc tccatcgctc tgggtcttcg acaggccatg atatctgttc
    14041 ctgggaagat ctctctgtga tgaatatact gattccgaaa ttctggaatg cgatgcatat
    14101 tgtataagaa agggtattct tcatgcgatg tctcatgggg cttttcgttt tgttgtcatt
    14161 accggccgtc ttcgggtgct ccagtatttc tgtaagttct gattataggg agtcgacgga
    14221 ctttagtgag ctgaagacat tttcatggat gctgaaaacg ccagaagggg agattgatct
    14281 tggtggggct aatcacatgg cgcggcaaag gattgagagt gcgatcgcag ccgaactggc
    14341 gaacaaggga tacattgaaa tagtcgctgg aaagtcagat ttccatgtga cctattatgt
    14401 tggtagagaa gagcgtattc aggtccaatc tatgggaggt ccactaatga ggccttactg
    14461 gggaatgggt atgggctatg cagaagttta ccaatacgag gaagggacgc tcattatcga
    14521 tctgcttgat gcgaaggcgc cgcagaatat tatctggcgt gggattgcta aaggcgtgat
    14581 ggattggaaa ggaaccacag gaggtcaaac cgggctgatc aatgaggccg tccagaaagt
    14641 ttttgcacaa ttccccccta agcgatcttg atgagggcgt ggtggtgtgt gttgattgac
    14701 caatggcccc cccgacgcgc ttcgcgatcg tggactcaac acgtcagtgt aattctcgct
    14761 cctggtggaa tagattcgct acgcttgcat cgtggtgtta tctcactgac cgcagctccc
    14821 ggcacggtaa ttctacatcg gaaaaatcat ttaccagaag attcggacat aggcgaatcg
    14881 gtcccatgtt tgtggaaggg atagatggtc ctccaatcct gtctcatgtc caccaccgcc
    14941 catcctttgc ctttcgcttc gtcaagggcc ttatcaagat ggccgatgga cgacttgcgg
    15001 tcataggccc gttcacgctc agggtccgtg tggtggataa gagccatgaa gcttgctcca
    15061 ctaccgctcg ccgcccactg gagcatttga aaatcgccgt ctgagttccc aaaggcaaga
    15121 atgggcctcc ggccgatgtg tgtttgaatg ccgacgggtt tgccgtcctt gtcgtcaatg
    15181 aaattgattt caggaagttt gaggagtact ggatatccat cgcgcaacgc gtatttcagc
    15241 ttgccgctgc tgccgatgac ttgttcgggg gggatttcat acacgtgctc cgtccatggg
    15301 cgcatgaact cgatgcctcc gcccgaaaaa atgaacgttg tgaattcatg tgcccggaga
    15361 tatgcacgca actccatcat ggactgatac accatttcac tgatcaagcg tccggtttgc
    15421 gggtgcttcg cggttgcgat ccaatcattc acggttttct cgaacttatc cctcgtcata
    15481 cccgcgtggg tgaccatgac gattttgagg attgcttttt cgccatctgc cagagcgccc
    15541 ttgatattcc ctttgagcag cgaggcgaac ggttccgtgt tacccattcg ggatgttgtg
    15601 gggcgagtgc tttgacgcag tcaagtgcaa agaagaattg gaagtacatg ggttgttccg
    15661 cccaaagtgt tccatcgttg tcaaaaacag caatgcagac agcttcaggg acaaggtcgg
    15721 gaccgggtgt ggtgacacgt ttgacgaaat ataccaagga ctgtttgttc ttgccgtcgt
    15781 tccaggatgg gagcacgtct tcggcgtaga tgagtggtgc ggtaaaaatc aagatgaaat
    15841 aaaccccggc agcgatgaaa gtccgcgtac cgatcgtttt cacccggggc tcctttccgt
    15901 gattgatatt tcgtttgaac gacgttctct tgataaggtt tacataacgt gaccgaaggc
    15961 aactgagaca tcagatggcc aacgccgtgc gaaggagagc tgccggccag agaccgttgc
    16021 cggtcagtcc caggccctgc ccccatacag taagactccg acgaccgcta cgttttagag
    16081 caacatggcc atcaccaatc ctgtcgctga ccggctctgt ccgtcattgc gtgcaaacca
    16141 gcaggcgacc gcaagggaaa agagcgcggc tcctgcactg caagccgtga tgagaccgcc
    16201 atctgaaccg agcgaagtac caaccagaac cgcaaccggt gccggtggcg agaggaagag
    16261 cgccaaaccg gtccctgcct caaggatcgc tgtcgtggtg aacagagatc tgagttgcac
    16321 agtgagtctt tccaatgagt gttttacgga ttagcccgca tccgccggag aggcggatgc
    16381 ggacccgtga actcaatcaa tgtttcagga cacctgatac tacttgttgc cttggcccac
    16441 ctaaagcgtt ttcagcatcg gatcgatggc gattcttgct gcggtggtag ggttgatgca
    16501 gctcaggtct ccctctccct gctctgagcc attgatcgtt atcccgagcc tgttgcccct
    16561 ggctccttga tagatgtgac ctactcggta tattgcacat gcaccgtctc gatcttaccg
    16621 tcgaatttga atggcgcctt atcgtaatag tctaacgaca ccggtgagcc gagatcggtg
    16681 ccgatatcga ggcagtcatt ggcggtaaac agcagcggtg cgctaatcgg gacgataccc
    16741 gaggcgactt ctttgccgtt gaccttcaag ataatcttaa gtggaccggc gggcttcggt
    16801 tcgacataac tggtcagaac ctcgatcttt accttaccgg cagaaagttt ctccttggcc
    16861 ttgatctgcg tgcgatgaat ctcgaacagg ttatattcgt aggacagcgt tccgtcttcc
    16921 atgtagaggg ccagtccccc ggaaaaaccg ccgagagcat agagcacgcc atccgcattc
    16981 gcagaaattt ccgtgtcgat ggtcacggtg tttggcttat tgccgagtgc gggcgcggta
    17041 aattccggca tccggataat gtcgccggag aacgtccatt ccttgtaggg tgtacttttt
    17101 cgcaactccg ggtgaagtgc caccacccag aggcctccgc cgatgggaag cgccccgttc
    17161 ttcgctgcct ccatcgcgaa cagctctttc atttgcgcaa gcttctcagg catagagtcc
    17221 gcaaggtcgt tggcctgggt ccaatcctct tcaatattgt agagctccca ccggtctttg
    17281 tcgggtgtcc agtcttggat gcccttaggt aggccgggga cccaagggat gcgggggccg
    17341 atcgcggacg ccatccagcc atcgtaataa atggcgcggc tgcccatcac ttcgaaatac
    17401 tgggtgtgca gtcggccctg agctttttca tcgttgagtg tataggcgaa actcgtcccg
    17461 tcgatcgggt cctgcgggat gccattgacc atccggggcg gggtaatgcc gaggatttca
    17521 tagatggtcg ggacgacgtc gttcacatga tgaaattgcg cgcgcggcgt cgcagagtgt
    17581 ttgatcttcg ccggccagcg cagggccata ggattgcgag tgccgccgaa gtgagcggcg
    17641 acgagcttgg tggacttgta cggcgtgctg cccgcccagg cccaacctgc atggtacatg
    17701 ttgtcggttt ttggtgaacc cagtacatcg aggccgccga gagcatcgag tgcctcaatg
    17761 tgttgcgaga tcttggtggg gattccgttt tgcgccagca gttcgctgat ggtgccgttc
    17821 tggccctccg cggaggagcc attgtcgccc cagatgtaaa aaatgagcgt gttctcccca
    17881 tacccgagtc ggtcgatctc atcgacaacc ttgccgacct gcacatccgt atgttcagcg
    17941 aatccggccg ccacttccat cagacggcgt tggaagggtt tctcatcttc agggatactg
    18001 tcccaagcgg ccagggtttc aggccgaggc gtcaattgcg cattctgcgg aatccaaccc
    18061 atttccttgg cgcgtaggaa tacacgctcg cgatatctgt cccatccgtc gtcgaacttg
    18121 cccttgtatt tgtcggccca ttctttcgcc acgtgatgcg gcccatggat ggcaccggga
    18181 gcccaataca tgaaaaacgg cttgtccggc gtaaaggctt tatgttgacg aagccatccg
    18241 atcgcatcat gggcgatgtc ctcagtcaag tgatagggtt ggccaggctt atgtgtgggc
    18301 tggacgaccg tggtgttgcg caccatatgg ggctcgtatt gagaggcttc gcccgcgaga
    18361 aaaccgtaga agtactcgaa gccatagccg gtcggccaat aatcaaacgg gccggcagcc
    18421 gtggtttgct ccgcgggagt attgtgccac ttgccccagg cgcctgtgtg atagccgtag
    18481 cttttgagga cgtcaggaat catcgcgctg ctttttggtt ggaggccgga atagccatcc
    18541 caatcgttgg caagttccgc aatctgtccg gccccgacgc gatgatggtt gcgtccggtc
    18601 agcaatgagg ctcgcgtggg cgagcacatg gctgtcgtgt ggaagcggtt gtaggaaatg
    18661 ccctgcttgg caatgcggtc gagggtgggg gtatgcactt cgccgccata ggtggccggg
    18721 aggccgggtc cggaatcgtc aataagtaca aggagaatgt ttggcacatc ggccggtaac
    18781 cgtttcggtt cgacgcgctg cctgtaaatt gactcctgca tggttcgtcc cgcgatactg
    18841 gccgaaggcg tcggtggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg
    18961 ccgcttgtga cggcttccat cacgttgtcc aggttgaaac taccgggttt ctggcgtggt
    19021 ggaaactcac ggaagctctg cagccacttc ccgacgtatc ctgcggcggg agcgaacatg
    19081 aacatgtgct cggcaaacca gcgatcgtag tccatgccga tctcatgggc atgctcgaaa
    19141 gggtccatgc gcaggttggt cagcatcggg gcgcgcagtt tcacaaacgg ctcctgccag
    19201 acacgcaggc cgtcggcgtc ctgccgcagg aacgtagcct tccagttacc ataacgcaac
    19261 gcggcgacat tgccatcgtc tgtccagtag atgaattcct tgcgcggcca ctcgccctct
    19321 cccttgaggg cgggcagcag gttgtagcca tcgatgtgca ctttgtaggt catatcgccg
    19381 atttgcctgc ccttcagcag atcttccttg accgtggtat cgcctgcagc ggcgagcaag
    19441 gtcgggagca tatcttcatg cgcagcaatg tcattgatca ccgtgccggg tttgatgaca
    19501 cccggccact tgattgcggt cggcacgcgg tagccgccct cccattgcgt gttcttctcc
    19561 ccgcggaaca tcgtcgtgcc gccgtcaggc caggagaaag attccgagcc gttgtctgtg
    19621 gaatacatga caatggtgtt ctcttcaagg ccaagttcct tgagcttgtc gagcacctga
    19681 ccgacatgac cgtcatgctc gaccatgcca tcagcatagg tccccagacc ggtttttccg
    19741 accgactcct ctttcaggtg cgtgaagatg tgcatgcggg tggagttcca ccagaggaaa
    19801 aacggtttgt cctccttcct ggcacgttcc atgaaatcca gggccttgac gttcacctcc
    19861 tcgtcgatcg tctccatgcg ctttttggtc agggggccgg tgtccgtgat gcggccatcg
    19921 gcgaaactgt gaatgacccc gcgcgggccg aaccgcttct tgaattcagg attcttcgga
    19981 tagtcgggat gttccggctc ctcctcggca ttgagatgat acaggttgcc gaagaactca
    20041 tcgaatccgt ggttcgtcgg caacatttca tcccggtccc cgagatggtt cttgccgaac
    20101 tggccggtca tatagccctt ggctttgagc aaggtggcaa tcgtcggatc ttccttcttc
    20161 atcccctcgg gagcgccagg caggccaacc ttggtgaggc cggtgcgcat gggagactgc
    20221 ccggtaatga aggccgcgcg tccggcggtg cagctttgct ggccatacca atcagtaaac
    20281 agcgcgccct ccttcgcgat acggtcaatg ttcggagtct ggtaacccat catgcccatg
    20341 ttataggcgc tgatgttgaa cccgccgatg tcatctcccc atatcacgag gatgttaggt
    20401 ttcccccctg ccatcgcaag gcttggggca atgaagatgg cgagtagccc gaaaagaata
    20461 cgacgaatcg agttcattct atttctcctt gatttgtggg acatgtgacc attcgaaata
    20521 tatggttaac ctacgggtgc tgatcgttgt tttccggtgc tatttatccg aatccttccc
    20581 atcaggtcat caatattttc gagacgtgga ttaggtgatg atcagccaaa ggaagaattc
    20641 gacgctgaac acactatttc ccttaaaata tccattaacc taccaggaaa aatggtgatc
    20701 atttcacccc gttttcggag ccaaatctat atgacgttgg caataactga tggcctcctc
    20761 acgaattcgg agcgaatagg actgaaattt gaaagaagag ttggaatgga aaaaaagatt
    20821 gttcacaaaa ttttttgtgc attttagaat ttatagagct catgcaattc agccatggaa
    20881 ggccatcagg gaggaccgaa tacgatgcgc ctcaaggtcc cttctcctaa ccgccgctca
    20941 atacctctat aaagaaaata ccccataaaa gccagcccga tgcatataac cttggcgaaa
    21001 atgagggaaa aatccttttg ttcccacacg gccatgaaag ccgcacccaa cagaccactc
    21061 tctcgaaaag catgaaacat cttttcagca aacaaaacca ggaaggtgca accactatac
    21121 accaaggtcc tatacagagc agccgcacca agcgaatgat gagcatcgaa cctggtcccc
    21181 gctcgggttt tacccaacag gaccacaacc tttgcaacaa tgagtgcgcc gaccagtgac
    21241 tttgagaaag catagaactc aatttcatag gcctctagca tcaatttcat gagggtgagg
    21301 acgatgccga aacaaaagaa aaaatacaga gtgaccaaac caacttcctt aacctcatgc
    21361 ttcaccgtcg aaaccattcc catcaggtat tctccacgtg tatgagtgaa atattcagaa
    21421 agtgttacct gagtgcaaac caaccatcaa agagccaatt agtactgggg ttccttcctg
    21481 gatactttga gtaagcctaa ccaattgcat cagatgttgt ctctgaaact cgtttatcca
    21541 aatgaaatac atcgcgaaag gtgtatgcat cctctaactt tttataaatt tactaaaact
    21601 ttctcattat gataatattt tgaggcattt aaggattatt ttttcccaaa ggaggataac
    21661 atggaagctg ttattggtgg tgttgtggtc ggagctgtcg tcgcagtggc tgtcccctcg
    21721 attgcgtctg gagtcggttc ggtagtgcgc cccatcgcta aaggaatgat taagggaggc
    21781 cttgtggcct acgccgccgt ttcagaactg gtttctgaaa ccggggaaca attcaatgat
    21841 atgattgctg aggcaaaagc ggaaatcggg aacaacgata aaaaaatgac cagggctaaa
    21901 tcgcagcggg cttgaccaca gccttagtcc aatcttctca ggaaatagcc tcctaagctt
    21961 ccagaaattt cattcaggaa gcagatgagg ctgttgccaa aattaaaatg tgatctgaag
    22021 tagtttttaa agtagttacc cggtggtcgt tagctactta tgtaagggca ggtttcaaag
    22081 gcaaggggcg gaggagcata atcccgtccc agtgggtttc ccattgccgg tttgaagtat
    22141 aggatcactc tcgcatttga ccgacccctt tacccgtgcc cgcgtctaat ctcgcaattg
    22201 aaacggtgga acgttccgat cacttctcct ggtcttgtga ggtccgtcat tctgggctgg
    22261 cttttttccg caatgaacca tattccatcc gaatcctcac tgctaaatta atgctgctat
    22321 aactacccca aaactggcat aggcgaccca agctgtcatg tttacctcag gaaggccaga
    22381 attattccga agaattgtgt cctgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnntcctt cccaatttac agacctttgg ttctttccca tggaaggtca gatgcatcga
    22561 tcagttttaa ggagggggca taagattcta aagttgttgg cctatgggaa ttcatggaaa
    22621 atttacgact ggagaggaag ggatatagag atctgtcggg attctggccc acccgttacg
    22681 cagcgcgggc catcctggta gcgaatggca atccctgtga gtgatctcac ggttgtgtca
    22741 tacatcatgg gcgtgcccat gccatagggt gaatacccgt atccaccaat cgtgtatccg
    22801 gttgtaccgt atccaccgta tccaaccatc ccgtaagaag aataccccca ccctgggtaa
    22861 ctgtagttgt aggcatagcc tccgtaggca ggggtcgcca cggttcgttt tgtccctcga
    22921 tgaagcacca cgccgtcagc ccccaaatca gcggcttttt tgagaatggc gccttcctcg
    22981 tcttcataac tcactgctga atccaggaca ctcaattggg caatggcgat atggggacat
    23041 tttggttcca gggccatcac ttccacatcg agtaccgatt ttttgggtgg aaaggtttgg
    23101 ctggtaaatc gaatggtatc gactgaggtg cacccggtca tgatgaacca tatccccgtc
    23161 aagcctatga gaagaaataa ggtcggtcgt ttaacacaat ccattgttgg ctccttgcct
    23221 tatcgcaaat attgttcaat cagtttaact tcgctttcta tgcgcaacac ttcgattcca
    23281 gcgcgctgtt gtcgataatc gttcatcgtc tccttaaatt ctttcagtcg gccaagccag
    23341 gtttgtaggg cttctggttt ttcatcggga tggtcctgga tcttcctcaa cagtttctcc
    23401 gtgaatcgca atccgtcgcg ttgcaccttc cattgttctt gatgcagttc ccaccctcgc
    23461 aggtcaagat tccggacttc ttgaatgaga ggatccttgg aatccggaat ctcatgtaaa
    23521 tactttattc gatccgaaat cttttcaatg ttgatggtta attccgcaat ctggcgttca
    23581 atatcgctga ggatggatgg caacgggtgc atttcctcat ttgcggccaa cgaggaacct
    23641 atgattccca tggttagggt cagcaccagt gcgaaaatgt tcgtcatgcg tgacatgctc
    23701 atagcctagg cctttctttg taatccaatt aggttggaaa ataatgcaaa ttctggaaag
    23761 ctggtttgtt tttccggcaa gggggttgct gaaagcccac cgcaaactga gggttaaatc
    23821 ggcatcctcg ttatcattcc atgctggcaa ccttcgaaac caagcgccat ttcaccataa
    23881 acccttctga tctcgcaatg aacgattcct tcaatcgata ttcatttgtg aggttctcag
    23941 cctatgagat ttcgtacaat agcgtggatg gcaatgtatg gttaaaacga atggcagata
    24001 tccgacaccc tcagacaagc ctgtggaacg tccacaatga atgaaccgtg gtggcttagg
    24061 aaaaaggaga ccatatgaaa tacgcaccga ataggattgc aagacaaccg gcactcactt
    24121 tcaggttgtg atgccacgat tgagaaacaa agacaaaggg aagactgatc agcccgctca
    24181 tcaggagcat gcccccaatc gaacccacac caaatatgag aatatagatc atgcccattg
    24241 ccatggatgg gattgtcgtc aggacagcaa gggttaaggc ggcactgcct gccatcccat
    24301 gcacaaaccc cactaacagg actttggcgc gagaaccgcc cagatgatgg gagtggctcc
    24361 catgatcctg cccatgaata tgaacatgtg aatgggtcat ttccttatga tgatgctcgt
    24421 gaaggtgaac cgtccatggc tggagggtcc tccacagaag atgcactccc aaagccacaa
    24481 tcatggctcc gaccataatt tccatgttgg ctgccatgtg tatgggaatc gtccacttga
    24541 gactcaacac cagaaagccg acaagggcta acgcacaggt gtgcccgact ccccagatca
    24601 ctcccaataa caccgaacga cggatatccc gtgattctgc agccaaggtt cccatggcaa
    24661 tgaggtggtc cgggtcgagc gcgtgcgtca tgcccaaagt gaggcccagc aaccatgaag
    24721 tgatcattag atgatccatt gaaagatggt acctggattc ggtgttttgg ccatcatacg
    24781 attcttacct gcttttctct ccccataata tgaacaatgt gcaaaacgac tctattccct
    24841 tcttcagtgc ctccggtgga ttcattcctt ttagggctag cccatcacat ggtcatccac
    24901 aactgttcga ccgcacacgt tttatcagat tgagccgtct tcctgctaca aataacaata
    24961 ttgaggttgg gatagccaag gcacaaagca ttgaagtggt tcagtacaaa aggacagaaa
    25021 gaaaagtgcg cgaataatct ctcggtctca tggtttttcc cgcctccttc tgaatatcga
    25081 cttccataat tctcctttct cgaacgatct ccttctggga catacttagg tccctcgtgt
    25141 cccaaccggc tgtggtgatc tagccatgaa acattcggat tagggttgtt caaaggaaaa
    25201 gacccgtttc cagtccgcct tcatgtttac gatggtccac ccgcgtttgg ccccttcatc
    25261 caggcctcgt gagagccgac cgatgtgcga ttctcgatca tacgcccatt cacgttcgtc
    25321 atcggtatgg tggaccagga gtccaaatcg aggcccttcc ccggtcgtag tccattcgag
    25381 catctggaag tctccgtcgg aattgccaaa agacatcagg gggcgacgcc ctatatattg
    25441 attgatcgcg accggtttcc cggccttgtc gttgaaaaaa ttgaattccc gcagacggac
    25501 gagtacgggt ttgccctctc ggacctcgta ttgagtcttg atgctacttc cgattacctg
    25561 ttcgggcggg ataccataga tgcgttcgac ccagggccgc atgaattcaa taccgcctcc
    25621 agaaacgata aaggttttga agccgtttga acgcagataa tctaataatt cgagcatggg
    25681 ttgatagacc atctccgtgt acaaacgctt cgtcttgggg tgcttggcgg tggcaatcca
    25741 ttccgtgacc gtttttctga aatcctcggt ggtcattccg gcatgtgtgg ctaagacgag
    25801 ttccagcaga gccttttccc cacccgcgag cgcctctttc acgttgccct tcaatatgga
    25861 agcgaagggt tcttgagttt tccattcggg atgctgcgga gccagcgcct tgaccctgtc
    25921 aagggcaaag atcgcctgga aatacatcgg ctgttcggcc cacaaggttc catcgttatc
    25981 gaataccgcg atgcgatcgt gtgagggaac gtagtccgga ctgccttctt ccgtaacttt
    26041 ggcgacaaac tgcagaatgg ctgatttagt
//
LOCUS       DAORAQ010000028        25523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_370_length_25523_cov_63.062353, whole genome shotgun sequence.
ACCESSION   DAORAQ010000028 DAORAQ010000000
VERSION     DAORAQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 25523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25523
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_25523_cov_63.062353"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(428..1102)
                     /locus_tag="PKM72_04495"
     CDS             complement(428..1102)
                     /locus_tag="PKM72_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNP60077.1"
                     /translation="MAGTNLFIPRYASIATLCALVIFSGCSQEYSTSIGEAVANEPQP
                     QLLPSDQQASKFDFTKDKPASQIYNFTMEDIDNKPRSLNEFKGQVMMIVNTASFCGNT
                     PQYAGLQTLYERYRDQGFTILAFPANDFGKQEPGDNKEIAEFCYTKYSLEFPLFSKIT
                     VIGEQKHPLYRYLTEDTSFKGEITWNFQKFLINRNGAVIARYDPKQKPLSPQIVDDIE
                     NTLRSS"
     gene            complement(1882..2211)
                     /locus_tag="PKM72_04500"
     CDS             complement(1882..2211)
                     /locus_tag="PKM72_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNP60078.1"
                     /translation="MIQGVVDREFDGMIEKSPVPVLVEFWQPDCGHCRALTKELEHLQ
                     KELGKRLLILTMNVQENFLIPGELEIHSLPTLALFLNGEFKQFIGGIGKKDQIIEQLS
                     PWLENSH"
     gene            complement(3110..4540)
                     /locus_tag="PKM72_04505"
     CDS             complement(3110..4540)
                     /locus_tag="PKM72_04505"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNP60079.1"
                     /translation="MTTNNSPVVVWFRQDLRIKDNPALLAAHKTGHPILLLFILDEEN
                     SGEWKLGAASRWWLHESLKVLNVTFGGKLCFKKGQAQKVLMDVIKEAGAAAIYWNRCY
                     EPWRIARDAHIQETLQTKGIEVHTCNGSLLFEPQTALKSDGTPYKVFTPFYQNGCLRN
                     RNEPRQPLKAPKNLRLSTYKGVALKDLDLLPNIPWHKKLAKHWEPGEAGAQARLKAFL
                     KSGLKHYKEGRNYPSRNNVSRLSPHLHFGEISPNAVWHAAKQHMGAEGWRKEGETFLS
                     ELGWREFSHSLLFHFPELPRKNLQKKFNTFPWRKDLKALKRWQQGQTGYPIVDAGMRE
                     LWGTGYMHNRIRMIVGSFLVKNLMLHWHLGEDWFWDTLVDADLANNSASWQWIAGCGA
                     DAAPYFRIFNPVTQGKKFDEDGEYVRRFIPELKKMPMKYLHNPWEAPEKVLAEAGVLL
                     GEDYPLPIVKLSDSRERALEAFSGLT"
     gene            complement(4693..5577)
                     /locus_tag="PKM72_04510"
     CDS             complement(4693..5577)
                     /locus_tag="PKM72_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009882 - blue light photoreceptor
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNP60080.1"
                     /translation="MIQISYISTATKPMSQEDLEQILRSSRENNARLGITGMLLYGNN
                     TFIQILEGEEQAVHELLARIKKDPRHTDFQVLKKKTVDRREYADWSMGFKRVSGEEFQ
                     AVKGLENLVEKDFNTTFLGSHESIVDSLMEHFRKDRLKKIGQEELSLDQEDRLIQVLH
                     QTIRAAVKVLAVLMVFTILWGVVDVVYLIYQKLILPSFTTFTIRDIVTTFGAFLAVLI
                     AIEIFINITLYIRKDVIHIKMVVATALMAIARKVIIFDFKEITPPYIFATAAVVLALG
                     ITYWLIERRFTWGEPDNL"
     gene            complement(6008..6964)
                     /locus_tag="PKM72_04515"
     CDS             complement(6008..6964)
                     /locus_tag="PKM72_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNP60081.1"
                     /translation="MKQPRYQHRMISILPIPGILIIAAFLAMIDIPLAVSQPVTIPIA
                     SIKGNIAMDAANPIWESVPGVVVPLSGQTITTPMHPNISVKTVMVKMATNGQDIGVWA
                     NWGDQTINNTTIGPQDFRDQMAIQFPVQDAGAPPFQCMGQSGGTVNIWRWNAEWQKDL
                     GMGVAGMWDVDQQYPAIAWDYYYEEPSGGVTYTNRTGRSAGPFNEGIWSGNIMSDPSL
                     RISSVEDLNANGFSTLTTQSAQNVLGNGLWEPYGALKGGCCNGPTWRVVMKRALTTDD
                     PNDVQFIAGSSFPVAFAVWDGSNVERNGMKGISTWFTAQMPN"
     gene            complement(7055..7693)
                     /locus_tag="PKM72_04520"
     CDS             complement(7055..7693)
                     /locus_tag="PKM72_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HNP60082.1"
                     /translation="MEQITPKFSDSEIHILECGLGWIKESEDAFADKLFHRLLREHPE
                     IATSLQTMGLQPFSRRIVQILDTIIKEIRLSGKIRSPIREQWADLAPTTVSHLQPHHL
                     LRMAETYLDIFSELAEDAWSPAMESAWRKIIHQVSISLWGHQPESLSFLALSSTFPFI
                     NRRNNRMSQPFALFWGAFIILAGSIASIGLWSRYRLAEVKLSKKPGLKKVWC"
     gene            complement(7914..9041)
                     /locus_tag="PKM72_04525"
                     /pseudo
     CDS             complement(7914..9041)
                     /locus_tag="PKM72_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(9232..9588)
                     /locus_tag="PKM72_04530"
     CDS             complement(9232..9588)
                     /locus_tag="PKM72_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNP60083.1"
                     /translation="MAPKGQSPLTRKRKPPSRPPKLDTIHHVAIPVPNVAKAVEWYTS
                     RFNCCVDYIDESWALLAFANTKLALVLPRYHPSHFALSRPDANKFGDLITHRDGLNSV
                     YITDPFGNSIEVMEES"
     gene            complement(9649..10155)
                     /locus_tag="PKM72_04535"
     CDS             complement(9649..10155)
                     /locus_tag="PKM72_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNP60084.1"
                     /translation="MADLKKAILLVGHGGIPKGYPSDLITKLKRLEAQRRAAGTPMSP
                     EELELDTKVRTWPRTPETDPYQAGLESLGAAMKPLLNGSLFGLAYNEFCGPTLAEAVE
                     SLIREGAETITVVSTMFTPGGSHSESEIPEELEELRKKHPKVTLIYAWPFNLEKVSRM
                     LMEHIQQF"
     gene            complement(10177..11136)
                     /locus_tag="PKM72_04540"
     CDS             complement(10177..11136)
                     /locus_tag="PKM72_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNP60085.1"
                     /translation="MSNIANDSQKFRIKSVASTTGLSAHVIRKWEERYQLVHPQRASN
                     GYRLFTEDDIQFLLYLKSQVDHGESIGQLAQAGEHELRQLMQHVPLDLSGIPSTYWND
                     AQEMIRSARRQDVQGITTILENWIDGLGLERALTIIIFPFLRLIGELWHQGGISLRGE
                     QSVSRLVRQHLINIIREESPLGRPHALIACVPGDFHEIGPLTASVLLRRIGWHSIYLG
                     PNVSFEMLEMALRRKHAQLIILSCIIEPGKGTLQTWIRDITQHLQPLCEVVVGGRGFT
                     PFAQLLGTHNISYFGQVQEVKTVQPRTHLLHMAKNISSSSFTT"
     gene            complement(11129..12163)
                     /locus_tag="PKM72_04545"
     CDS             complement(11129..12163)
                     /locus_tag="PKM72_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNP60086.1"
                     /translation="MTTPLIDIDHKPHKKTTRPDNNMAEPLSDHKPRLGISQCLLGDT
                     VRYDGGHKRDAFLTDVLAPFVEWLPVCPEVEAGLSTPREAMHLAGDPEAPQLLTIRSH
                     IDHTATLHDFSQRRVQELQESDLDGYIFKKSSPSCGVYRVKVYTDKGQPSKQGTGIFA
                     SAFQHAFPLLPVEEEGRLSDAPLRENFIERIFCYRRWKTLFQEHRISRGAIVQFHTRH
                     KYLLLTHSRSHYHELGQLIAQAGQYTAGDLANRYGPLFMDALKIKATVRKHVNVLQHL
                     AGYFKKQLSTIERAELQETIQDYHRHLTPLAVPLTLIKHYVRILAVPYLMDQVYLNPH
                     PKELMLRNHV"
     gene            complement(12370..12819)
                     /locus_tag="PKM72_04550"
     CDS             complement(12370..12819)
                     /locus_tag="PKM72_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNP60087.1"
                     /translation="MLPCFVMALILVGSFAPGSLAYEEADEKPPTSSNPHASPPGANT
                     PAPSGIVGLALHITAVRIGEPARLIIRAVHPSGPAARAGLNHGEEILTVDGLPLAGKT
                     YQEAVGLIRGEVGSTVRLGLTGPQGERTVSVVRINESVLMEQTPQAM"
     gene            complement(12884..13360)
                     /locus_tag="PKM72_04555"
     CDS             complement(12884..13360)
                     /locus_tag="PKM72_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNP60088.1"
                     /translation="MTEHILIFFNLLAMAILVGKIVFLSFVTAPVLSRTLDTDTFAKV
                     VRSLFPQYYGLGMISAAVGWTTSIALGLLNGFESIVLLPTTIWLGVLAIEHYCRTPLI
                     AQINDLSDQLKAKQARGLTTPLLQQQRDGLHRLSVQLNSLVLFMGLCLMGLFGISP"
     gene            complement(13452..13709)
                     /locus_tag="PKM72_04560"
     CDS             complement(13452..13709)
                     /locus_tag="PKM72_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60089.1"
                     /translation="MCDEQTHRFSERNGGLDHLMTFVTLSTIAYNDQQEFSAKANIHH
                     IPFLADVLEYRFGTQTGLLLEDLSILRRDAHTGCQGQYSIC"
     gene            complement(14006..14923)
                     /locus_tag="PKM72_04565"
     CDS             complement(14006..14923)
                     /locus_tag="PKM72_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNP60090.1"
                     /translation="MKMVIAGGTGFIGREVCRMLIEEGHSVIILTRHAENYTASATPQ
                     RHFVQWDGVTLGPWAQECEGADVVINLSGAPIADSRWTDKRKQVLIDSRVEPIQALLL
                     AIPTWKHKPHTFISASGVGFYGARGDEELNESSTRGKGFLSDLSQVWENTAMEAEALD
                     LRVIPVRFGMVLGPDGGALSKMTVPFRVFLGGPVLPGTQYVSWIHRNDLARLILFVIT
                     HPTLDGTVNGVAPEAVTMQDFCTTLGKTMNRPSWLPVPGFALKIALGELATMLTTGQR
                     VHPQKALDSGFSYSFPTLQKALQSILGKS"
     gene            complement(14934..15902)
                     /locus_tag="PKM72_04570"
     CDS             complement(14934..15902)
                     /locus_tag="PKM72_04570"
                     /inference="COORDINATES: protein motif:HMM:NF026037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60091.1"
                     /translation="MLILRTEPLFPARGTILAVFAVTFCAVLNPVHSGFAYEEIPNFA
                     GGDLKGSVFMKGTLHSPRRYNLVLSADPYYCGRISDGKGWRLSPITRPGPNHSLPGAV
                     VYLENVPRGKAAPSTPAMVQTKDCMFLPHVGTAKAGQDLHFQNWDPVEHKLEIFLTSA
                     TGGLHLFGQTLPPHPDNRKSDFLSEGKTGTHRAGTKVTYAVEKTGIILFRCSYHEFME
                     GWGIVLPHPYVTQAGKQGEFSITDIPPGTYNLVIWHPMGQTITSIHILPGRTLNVDVD
                     VSSTSTTAYPEDTHKPDPFGIDLVGDAHIVPTVELQEWDAPSEIMR"
     gene            complement(15956..16543)
                     /locus_tag="PKM72_04575"
     CDS             complement(15956..16543)
                     /locus_tag="PKM72_04575"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNP60092.1"
                     /translation="MKTLQVLCLTSAVILLGFSMSDIAASEEGTHSIKGVRPEIVADF
                     IYAVIESDRTLYTTHIVDRMQETGTVIASETWESRNALPLPAQMLLLSGSAVKEKGIG
                     LEYRLASRLPIYQKNGPASDFEEAGLAAVEREPSQPATGYITKGEQTYFQAIYADRAI
                     SRACVTCHNTHLLSPKRDRKLGDVMGGIIISFPVE"
     gene            complement(16564..17391)
                     /locus_tag="PKM72_04580"
     CDS             complement(16564..17391)
                     /locus_tag="PKM72_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNP60093.1"
                     /translation="MKMWCLMIIALMLTVTPPTATAAEATTIEKDPVEMMTIPAGPFI
                     RGSAPGEGRLDEQPRRKIYLNAFAIDTYEVSNAHYMKFLDETLHKPPMNVFAERPFNK
                     EEGIAELPVVQVTWHDAVDYCFWAGKRLPTEAEWEKAARGNDGRLYPWGDQAPTPKQA
                     NFERDWEEKKTFVEVTALPEGQSPYGLRNLAGNVREWVQDWYASEYYASAPDKNPKGP
                     ETGILKVLRGGSWKSFDTDLRATSRGKGGFALKTHGIGFRCARDIGLGQTESESNTQ"
     gene            complement(17388..17771)
                     /locus_tag="PKM72_04585"
     CDS             complement(17388..17771)
                     /locus_tag="PKM72_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60094.1"
                     /translation="MNMRVASSYLAGILFLSCIPMIQGGTPVYGVPPVAAQEQEGSRG
                     LIYQASQATEQAWEEFHAAAIGGTLASPAVQSSIERQLHEARALLMEARKAERDNQFD
                     AVNTLTQKIMQITQNIVQASREKKQ"
     gene            complement(17815..18930)
                     /locus_tag="PKM72_04590"
     CDS             complement(17815..18930)
                     /locus_tag="PKM72_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNP60095.1"
                     /translation="MTNIKSGEVRNEKLTRSGGRLPVAGVLLGSLLLTLHPSAAFAQD
                     SAVVPDKMSYVPFGPSIMGIDKDPSKAASTTGDSATLYQRRMSMPWSKEAFHDEGPAH
                     WVVVDGYFIDKYEVSNSNYAEFMKTKGHPGPAYWDDPRLNKPNQPVVGVNWFDAKAYC
                     EFKGKRLPTEAEWERAARGPEGLKYPWGNEFDAAKANFGKNHAATLPIDSMPEAASPY
                     GLHHMAGNVFEWVEDWYDPDFYRKTPHSVNTMGPLKPIWIGGTGTYVDRLTVGEKRVI
                     RGGSWIAAQSSITTTHRFWNHPSNNSYGVGLGFRCAKSASTVVNDKVRTATIDAMKRM
                     GQEKWQEAKTQIATALELDPHNTELQQMQAIVQDNIR"
     gene            complement(19003..19953)
                     /locus_tag="PKM72_04595"
     CDS             complement(19003..19953)
                     /locus_tag="PKM72_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNP60096.1"
                     /translation="MKPFHQTIGGTMMASLLLITTPALSAGPENMVLVPKGEFVMGSE
                     NHQDEKPHNVVLDAYHIDTYEVSNKDYKEFMKNTGHAAPAYWDDPRLNKPEQPVVGVN
                     WYDSSAFCEWKGKRLPTEAEWERAAKGPDGHAHYPWGHELDASKANYGQTVGQTTAVD
                     SYPEGRSGFGAYNMAGNVFEWVSDWYDPGYFNVSPAMNPQGPSTGLNFANQGQVKVLK
                     GGSWLAPASSLHTSHRFWNQPENNSYGVGLGFRCAQAASPAGLQNVQYDFMLSLISMG
                     QEKWQEALTSIEKALKADPKNEEYLQTQTLIMHQINKDKK"
     gene            20425..21324
                     /locus_tag="PKM72_04600"
     CDS             20425..21324
                     /locus_tag="PKM72_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNP60097.1"
                     /translation="MPMYRIHRFSKLTGLSPHVIRAWERRYGLVNPVRGDNRYRLYTD
                     DDVVLFRYLKSQIDQGVSIGELSVQGREHLQEQAQRVFVETHREPPPSERLVTDLSQA
                     LEENDRVGFERKLNGALAVIPFEEALHRFLLPLQERVGQLWHDGKLGVGQEHYASNQV
                     KQKIFSALNHFQTSDVGPVVVIACPTNEWHEVAAMTAAYVCRARGCRVHYLGANLPIP
                     ELAAYCDRNRPTCVLLSVTMMESSEEAIALAQEIAGTIVPLTPVGLGGQAIQAYAEAF
                     LQENIIVFQDLKALDRFVVSLKH"
     gene            21519..21974
                     /locus_tag="PKM72_04605"
     CDS             21519..21974
                     /locus_tag="PKM72_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNP60098.1"
                     /translation="MENDLYQIIYASAATRRIEEDELLSMLKHARNKNKQLGITGMLL
                     HCDGSFIQALEGPKDQVMNMLGVIRQDSRHNRIVVLFQGPIKNRSFSQWSMGFNRPSQ
                     EKLATSEGYTPYLDVGDDSQAINNYSSVALKLIGAFRELSDKQSSSMEG"
     gene            22405..23220
                     /locus_tag="PKM72_04610"
     CDS             22405..23220
                     /locus_tag="PKM72_04610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNP60099.1"
                     /translation="MPLPVLCSEDSPPHAPPRLKPWRHAWSAVLIVGCFYVVQALGIF
                     MVSAVGGAVETLAGGIDVGAQFGTVPQYKEVWLLPLSLCVGTLGAMMVSIDLARRRAV
                     LSSNANGFSELLGKSYDFRNLWRFVLMGLALGFGFVAFTEYGGLPADDLPQPLFDAMI
                     SAPPSLQTLWALMIVGVFPVVEEVLFRGILFTGMSESWGPGLAGFMTTMIFVGVHMPK
                     VLEYWPALLAVSLVGSLTILLRIRTESLVPGIALHGTYNGVLVMVAFLTQATS"
     gene            23666..24556
                     /locus_tag="PKM72_04615"
     CDS             23666..24556
                     /locus_tag="PKM72_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNP60100.1"
                     /translation="MHTGDVERMVQLAEKKVGYFRSNPIGYLVLSALAGIYLGFGICL
                     IFSVGAPFWAEGSAGFKLVMGVSFGIALTLVIFAGSELFTGNNMVCALGALAKAITMK
                     QVGQIFFWSFVGNLAGSMGLAWLVVQSGVVGQAPQSDLLLKVAALKMNLPMWELFIRG
                     VLCNWLVCLAVWTASRTTNDVAKIMLIFWCLFAFIGSGFEHSIANQSLLGIALFLPHG
                     PDISWTGFLWNQLWVVSGNIVGGVVFMGGAYWIISPVRGWIKKAEAVAEGASLESSRR
                     IPVPAERELLSQPLAVGREN"
     gene            24610..>25523
                     /locus_tag="PKM72_04620"
     CDS             24610..>25523
                     /locus_tag="PKM72_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HNP60101.1"
                     /translation="MNKIEVLKGEKDGLDIKEDIARYAESGWEAIPDEDIQRLKWYGL
                     FLRNPTPGFFMIRVRIPGGRTTSVQLRTLAHLANTYGNGVLDLTTRQQFQLRHLKIEH
                     VPEVFRQMEEAGLCSLQTGMDNVRNIMTCPVAGLTTQEQLDATDLVKRLTEALVGHRA
                     YSNLPRKFNLAITGCLDNCLHLETQDLALVPAMLEGRDGAVIGFNLLAGGKLGSGGYR
                     IATPLDVFVSPQEVVAVTGAILRVFRNHGCRDSRTTARLAFLLDDWGEERFRMEVQRE
                     VGWELSRAGTDSRKHSVNDHMGVYRQKQP"
BASE COUNT         6090 a         5975 c         6603 g         6855 t
ORIGIN      
        1 aattcccggc ggtgaacggg gccgccgact ttttttcgca acgcttccga cagcctatgc
       61 tgacccagcg agagataggc tactactcgc ttcaaccgac cattttcttc ttccaactgc
      121 cggagtcgcc ggaattcact caccccaaat aggcatactt tttcttccac acagaaaacg
      181 tcgtctttgc cacgcttggt tggcggcaga tgttccccac cgaggtgtcc tcttcctctt
      241 ggggaagggc agagatgacc ggtcctccga gaattccgaa tgcttcatag atttttcctt
      301 tcgtgttgga ccaccgaagc tgccttttta ctctcgttct gatgggcgtt catttttggg
      361 gcagacgtca attggttcta tgagttgcat gcaggagtag caataatatt gagaaacgat
      421 cttaggttta ggaagatcgt agagtatttt ctatatcgtc cacaatctgt ggagacaggg
      481 gcttctgttt aggatcatac cgagcaatga ccgctccgtt ccggttgata aggaattttt
      541 ggaaattcca ggtgatctcc cctttaaagc tggtgtcttc ggtgagatag cgatagaggg
      601 ggtgcttctg ttcaccaatc accgtgattt tactaaacaa cgggaattcc aaggaatact
      661 ttgtatagca aaattccgcg atttccttgt tgtctccagg ttcctgtttg ccaaagtcat
      721 tcgccggaaa ggccaaaatc gtaaatcctt gatcgcggta tcgttcatat aaggtttgga
      781 gtccggcata ctgcggcgta tttccacaaa agcttgccgt attcacgatc atcatgacct
      841 gtcctttgaa ctcgttgagg gagcgtggtt tattatcaat atcttccatg gtgaagttgt
      901 aaatttgtga agccggttta tcctttgtga aatcaaactt tgaagcttgt tgatctgaag
      961 ggagtagttg gggctggggt tcattggcaa ctgcttctcc tatagatgtt gaatactcct
     1021 gactacatcc gctaaagatt actagcgcac acagtgtggc tattgaggca taacgaggaa
     1081 tgaatagatt tgtgcctgcc atggtgaatc ctcctgagat aatggacaac cagtcattcg
     1141 tttactccag tgtaattctg aacctccggt cagggcaatt ggcccagata gaaatatcaa
     1201 ctggactatt aggtgtttga actttttcct aataaaccca tactgggttc tagaaaaata
     1261 gccaaaggca tttgtgcttg ataatatgat cggaaatggg attaacagaa agtcgctggc
     1321 cccgccttcg atagccttaa tcatcgtcga ccgagtggga tctgcaggca tcatgaccac
     1381 tgaaatggtc agatcttttc cagagttgtt ttagcgtcgt caagtcctcg atgatcggca
     1441 tttcaaggtc aaggaaaatc tcattaacgg attccgtttt aagggccgta agtctctcca
     1501 ggttgttcga ggaaggtgga aacttcaaat ctaatttttc tcaatctgtc gtgaagaaaa
     1561 aattatagtt tcctgttcat cgactgccag aattcgttcg ccgataggag gtacacctga
     1621 tttgtaaacc aacatttctc tatggtttat tgctttttat ctttatcact atcatatcga
     1681 cttcatccct tgtgggggcc aaagaaatat aatgaaacgt tagacaattt ggagtaacta
     1741 gagggtatgg gggaaggcat atgacgtgaa gaaagaagcc ctcatcgtaa tgagcctggc
     1801 atagctactc caagtgtgaa acatccttga atcacattaa gaaagaaacc cgtaaaaatt
     1861 ttcaataaac ccaaaaccgg gctaatggga attttcaagc caaggagaga gctgctcgat
     1921 gatctgatcc tttttgccga tcccaccaat gaattgcttg aattccccat tcagaaatag
     1981 ggctaaggta ggaagggaat gaatctctaa ttccccggga atgaggaaat tctcctgcac
     2041 gttcattgtg aggatgagaa ggcgtttccc aagctctttc tgcaagtgct ctaattcttt
     2101 ggtcagagct cgacaatggc cacagtcggg ctgccaaaat tctactaaaa cggggacggg
     2161 cgatttttca atcatcccat caaactctcg atcaactact ccctgaatca tgttcgccaa
     2221 ccttgattac ttaatgctgc ctgggaaatt cgcgcatttt ttggaaacaa tgaatgatca
     2281 tgatgttaac tgatttgtaa ggataggttg aagaatttgc ctctggtttt gggtatcttt
     2341 tgggttgtag ggtcttctcc cggtttccca gtgagacttg atgggcacca tgtcaggacc
     2401 atgggaggct tcccggctca acgggcctat tttatcatga cgttgatctt ctaggacgat
     2461 aacccagttt cactttctca tgccttttgt ggatgacttc aatttgttgt gaaattcatt
     2521 catgaggcct ttagtaaatt taggaaaatg gaatccgtgc gatcattaat gtaagtatga
     2581 gtaggaaggt tacggaaaat tttctgccta gatgaatact attaggcacg gccagtcagt
     2641 cgcaatcgat ttttggcaaa ccaggtatat gtgttcacca agagtttatt gaagattggt
     2701 cttcggctac accgagcaag aaagcctagc ccaactccct cccacatttc cctgaacacc
     2761 tcgacacctg taataagggt tccatccgac cagcgggcat ggatcacttt ctccaaatta
     2821 cattggctca gtccatggtc tgtggcatga taagacgaat ttgaaaaatc aatgaaatga
     2881 agatgggctt ttcgattgaa cattttcatc agatcgatct ctcgacgaca aatcgggcat
     2941 tcgccgtcat aatagaccct cagtgggtac ggcttcgtgc aatggaggcg tggctgtgtc
     3001 ttcgggttca aagcaggttt tgttttttaa gatctcatga atgactcttg aaaaccaggg
     3061 aagtccccgt attcaattgc gaccgagttg tatggggcat ttgcgatgct taagtcaatc
     3121 ccgaaaatgc ttccagcgct cgctcacggg aatcgctgag tttgacgatc ggcaggggat
     3181 aatcctctcc gagtagcacc cccgcttctg ccaacacttt ttcgggtgct tcccaaggat
     3241 tatgcaggta tttcatcggc atctttttta actcggggat gaaccgtcga acatactcgc
     3301 catcttcatc gaactttttt ccctgcgtga cggggttgaa aattctaaaa tagggtgccg
     3361 catcggcccc acagcctgca atccattgcc agctggcact gttgttggcc agatcggcat
     3421 cgaccaatgt atcccaaaac cagtcttccc caaggtgcca gtgcaacatg aggtttttaa
     3481 ccagaaagga tccgacaatc atgcgaatac ggttatgcat gtaaccggtt ccccacagtt
     3541 cacgcatgcc ggcatccacg atggggtagc cggtttgacc ttgttgccat cttttcaggg
     3601 cttttagatc tttacgccag ggaaaggtat taaatttctt ttgcagattt tttcggggga
     3661 gctccgggaa gtgaaagagc agactgtgag aaaattcccg ccagcccagt tcacttaaaa
     3721 acgtctcccc ctccttccgc catccttcag cgcccatgtg ttgtttggcg gcatgccaca
     3781 ccgcattcgg tgagatttct ccgaaatgca gatgcggcga taaccgcgag acattgtttc
     3841 tggaggggta attccggcct tctttgtagt gtttgaggcc gcttttgaga aacgctttca
     3901 gtcgagcctg tgccccggct tctccgggct cccaatgttt ggcaagtttt ttgtgccagg
     3961 gaatatttgg cagcaggtcc agatctttca gtgccacacc tttgtatgtg gaaagcctta
     4021 aattttttgg ggcttttagg ggttgtcggg gttcattgcg atttctgaga catccatttt
     4081 gataaaaggg agtgaacacc ttatacggag tgccgtcgct tttgagcgct gtctggggtt
     4141 caaaaagcag ggagccgtta caggtgtgaa cctcgattcc tttggtctgc aatgtttctt
     4201 gtatgtgtgc atccctggcg attcgccagg gttcgtaaca tcggttccaa taaattgccg
     4261 ctgctcctgc ctctttgatg acgtccatca ggaccttttg agcctgtcct ttcttgaagc
     4321 acagcttgcc cccgaatgtt acgttcagca ccttgagact ttcatgcagc caccagcggc
     4381 tggcagcgcc taacttccat tctccgctat tttcctcatc aagaataaat agtaggagga
     4441 tagggtgccc tgttttatgg gccgccaaca atgccgggtt gtccttgatg cgtaaatcct
     4501 ggcgaaacca gaccacgact ggggagttgt ttgttgtcat gctatgcctt tggcacaatt
     4561 caaagccgaa aattcaatgc tccacactaa ctaaaaaaaa atccgtgatt accatttggc
     4621 taaatcctga ttcagccaaa ggatggtatt gggttgaatt ccgatggagg gggcaaccca
     4681 acattcgttg tattacagat tatctggttc tccccaggta aaccgtcttt caatgagcca
     4741 gtaggtaatg cctagcgcca gcaccaccgc agcagttgca aaaatatatg gaggtgtgat
     4801 ttctttgaaa tcgaaaatga tcacctttcg ggctatggcc attaaagctg tagctacgac
     4861 cattttaatg tgaatgacgt ctttgcgtat atacagcgta atgttaatga aaatttctat
     4921 ggcaatgaga acggctagaa atgctccaaa tgtggtgacg atatccctaa tggtaaatgt
     4981 cgtgaaactc ggcaatatga gtttttgata aatgaggtac accacatcaa cgactcccca
     5041 caaaatggta aacaccatca agactgccag gacttttacg gctgcccgta tggtctggtg
     5101 gagaacttgt atgagacgat cttcttgatc gagggataat tcttcctgcc cgattttttt
     5161 gagccggtct ttgcggaaat gctccatcag ggaatccaca atactctcgt ggcttcccaa
     5221 aaaagtcgta ttgaaatcct tctcaacgag gttttcgagg cctttcacgg cctgaaattc
     5281 ttctccggat acccttttaa atcccatgct ccagtcggca tactcacgac gatccacggt
     5341 ttttttcttc agcacttgaa agtcggtgtg gcgaggatct tttttgattc tcgcaagcaa
     5401 ttcatgtaca gcctgctctt ccccttccag tatttggatg aaggtattgt ttccatacag
     5461 caacattccg gttatgccta atcgtgcatt gttttcacgg ctgctcctta gaatctgttc
     5521 caaatcctct tgtgacatcg gtttggtggc ggtactgatg tacgaaattt ggatcattaa
     5581 tttaatcccc cttcacatga gcgaatggtc tatgatgttg ttttttaatt gtaaccatag
     5641 gggaatgagt ccgataggtc atatggcaca gaagggggat aggaataaga acagtttcca
     5701 tctcaagccg taaaaaaagt acccaggctt tagcgggagg aattattcct cgtggcgtgg
     5761 agtaatttat actgaattga gggagagctc cacacttgaa aggctgaggt taaaagcaag
     5821 gtcaattttc tcgatctcat gggcacctga taggcgcagt ttgtattctc aaattttcat
     5881 gccccgatta acgtggaaac acaaagccct tcagaggcct acccgctaaa ccctcacaat
     5941 actttccctt ggactctagg gccttggtga gatttggcac tcctgaagag tttattgcaa
     6001 tattccgcta atttggcatt tgagccgtga accaggtcga aatacctttc atgccattac
     6061 gctctacatt cgagccgtcc cacacggcaa aggccactgg aaagcttgaa ccagcaataa
     6121 actggacatc attgggatca tcagtcgtca gggctcgttt catgacgacg cgccacgtcg
     6181 gtccattaca acaacctcct ttaagggcgc cataaggttc ccataatccg ttgcccagca
     6241 cgttttgtgc tgactgggtg gtgagagtgc taaagccatt ggcgttgaga tcttctacag
     6301 aactgatccg gagggaggga tctgacatga tattgcctga ccagatgccc tcattaaaag
     6361 gcccggcgct tcgtccggtt cgattcgtat aggtgacccc tcctgagggc tcctcataat
     6421 aataatccca ggcaatggcc gggtattgtt ggtccacgtc ccacatgccg gccactccca
     6481 tgcctaggtc tttttgccat tcagcgttcc atcgccaaat attcacggtt ccccctgatt
     6541 gccccatgca ttgaaatggg ggagctccgg catcctgaac cggaaactgg atggccattt
     6601 gatcccgaaa atcttgaggc ccgatagtgg tgttgttaat ggtctgatca ccccagtttg
     6661 cccagacgcc aatgtcttgt ccgttggtgg ccatcttcac catcacggtc ttaaccgaaa
     6721 tattcgggtg catgggggtg gtaatggtct gaccgctcaa tggaacgaca actccgggga
     6781 cggactccca aattggattt gccgcatcca tcgcgatgtt tcccttgatg gaagcaatgg
     6841 gaatcgtcac cggttggctc acggctaggg ggatgtcaat catggctaag aacgccgcta
     6901 taatcagaat tccagggatt ggcaggattg aaatcattcg gtgttgatac ctaggctgtt
     6961 tcatgttgct cctgttcgct gaatcattaa atctcaatgt tcctaattca tggctcgcct
     7021 cgttttggaa gagtctcatc acatcagtgg gtaatcagca ccaaactttt tttagccctg
     7081 gcttctttga cagctttact tctgcgaggc gataacgact ccacaggccg atagaggcga
     7141 tgctccccgc caaaattatg aaggctcccc aaaaaagtgc aaatggttgg ctcatacgat
     7201 tatttctcct gttgatgaat ggaaaggtcg atgaaagtgc taaaaaggaa agggattcag
     7261 gttggtgtcc ccacaaactg atgctcacct ggtgaatgat ttttctccag gcagattcca
     7321 tggcagggct ccaggcatct tctgccaatt cagaaaaaat gtctaaatag gtctccgcca
     7381 tcctgagcag gtgatgaggt tgcaggtggc ttaccgttgt gggtgccaga tctgcccatt
     7441 gttctctgat gggtgaccga atttttccgc ttaagcggat ttccttgatg atcgtatcga
     7501 ggatctggac gatgcgcctg ctgaatggtt gtagccccat tgtttggaga gaagtggcga
     7561 tctcaggatg ctctcgcaac aagcgatgga acagcttgtc ggcaaatgca tcttcacttt
     7621 ccttgatcca tcctaggccg cattcaagta tatgaatctc cgaatctgaa aacttggggg
     7681 taatttgttc catgggagaa gtgcctttcc tatggttctc taaatatagc ttctttttca
     7741 atgtacaccc tggggtctag gggaggtcaa agaaaaatga aaaaccttcg acaacgaaag
     7801 gccgctcaag acttgcgtgc gcatgtcgga aggtagaccg tctcaatgac gactatggag
     7861 attgaagtgt tgttgggtcg gggaattcta gaaaacgggg aggatgccgg atatcaggtc
     7921 tctagccatt ttcgaatctt ttctgccgca cgacgaccgg aaaaaagggc tccatccata
     7981 gtcccggtgg cagaaaaatc cccacaaaca acgactccat cctgaatatg tgtctgacga
     8041 gagaacgggt taggaaatgg tggaacctgg gcaggttggg ctgcggtgat ggaataggct
     8101 cgaattggtt gccagccttc gacttgtgag ccaaaccatt catgcaattg ttggcgaacc
     8161 cgggtggcaa ggtctcctat ggaaggatca gcatggttta acactgaaac ggagatcaat
     8221 tgtttgttgg acgtggaata ggtcggggcg atttgagtga ggacacaaag attgttaatt
     8281 ggaccgactc cctctccgtt caataacaga aacggccctg aaaaaggcgg ctcagaggta
     8341 gcatagtaga gacatgttac atggcgatag ggagctggat tgagtccggg aacaagcctg
     8401 gttgcggccg gtccgtcggt ggccacgaca atggcgcgag cggatagagc ctcccctgat
     8461 tccaaggtta ccgaagtctt ttgcatggat gtgaccctgg cttgagtgcg aattcgccct
     8521 gccggaagac gggataccaa ttgtcgggga atggctccca tccctccggc gggaagtgaa
     8581 atgtctccag acgcaaatat ccgaaaagca aattccgcca tacgactgga agtctgtaaa
     8641 tccgaatcca aaaatacgcc accaaggaat ggtcgaaaaa atcgttcgac tatggagtaa
     8701 gaaaatccga aatttttaag gtgttggagt gttgaggttt cgggcctttg aaataaatcg
     8761 ttcaagcttc ctgtatttgc ccgatggcga aatgttgtta cccggatttt gtctgaaagg
     8821 gtgccgatag gagtgaagag cgcttgaatt ccctgaaaag gatgtcagaa gggatccggc
     8881 atccgatgcc atttaccaag acaccgaatc actgcacctg ggaaaaattg atggagctcg
     8941 agggcagggt aattcaatat gcgtttggcc tcaggatacg cagtggaaaa gacctggaaa
     9001 ccccggtcta gaaggaaacc atcaacgtga tctgtgcgaa tcctcccccc ctacgtcttc
     9061 tgatgcttca agcagtaaaa agtttatccc ataatccgct aatgttttcg cgcaggaaag
     9121 tccggcaaga cctgctccga aaagggcaca tcagcattca tgggagaaat atgttggttg
     9181 aggccgagaa aggaaaaagg tatatgcctg atgttcggaa agtcgtgcaa attatgactc
     9241 ttccatcact tcaattgaat tgccgaaggg atcggtgata tagactgaat tgagtccgtc
     9301 ccggtgggtg atgagatctc caaatttgtt ggcatctgga cggctgagcg caaaatgaga
     9361 aggatggtac cgaggcagaa caagggccag tttggtattg gcgaaggcca gaagcgccca
     9421 ggattcatcg atgtagtcca cgcaacagtt gaagcgggag gtataccatt cgaccgcttt
     9481 ggccacgttc gggactggaa tcgccacatg atggatggta tccagttttg ggggcctgga
     9541 cggtggcttt cgctttctgg tcaaggggct ttgccctttg ggtgccatgc gcttgtgtct
     9601 acctctctac gcgtattgaa cggtggatga aaaaccttat taagcctatc aaaattgttg
     9661 gatatgctcc atcagcattc ttgagacctt ctccaggttg aacggccagg catagatgag
     9721 agtcaccttc ggatgtttct ttctaagttc ctccagttct tcggggattt cactttcgga
     9781 atgcgatcct cccggagtaa acatggtcga aactacggtg atagtctctg ctccctcgcg
     9841 aatgagggac tcaacggctt cggcgagggt cggtccgcag aactcattat aggccaagcc
     9901 aaacaacgat ccgttcaaga gtggcttcat ggcagcccca agggattcga ggccggcctg
     9961 gtaaggatcg gtctcagggg tccgtggcca agtacgaacc ttggtatcca attctaattc
    10021 ttcaggtgac atgggagtgc cggctgctct tcgctgggct tcaaggcgtt ttaatttggt
    10081 aatgaggtcc gatggatatc ctttagggat gcctccgtgt ccgacaagaa gaatagcttt
    10141 tttaaggtct gccataaatc aaactccttc catagactag gtggtgaatg aggacgagct
    10201 tatgttcttt gccatgtgaa gaaggtgagt tcggggttga accgttttga cttcttgaac
    10261 ctgaccaaaa taggaaatat tgtgggtgcc gagtagttgg gcgaagggtg tgaacccgcg
    10321 tccgcccacc actacctcac acaatggctg aagatgttga gtaatatcac gaatccaggt
    10381 ttgtagtgtg cctttacccg gttcaataat gcaggaaaga ataataagct gtgcgtgttt
    10441 tcggcgcaag gccatttcca acatctcaaa tgagacattg ggcccgagat agatactatg
    10501 ccatcctatt cgtcgtaata gcaccgaagc tgtgagcgga ccgatttcat gaaagtctcc
    10561 aggcacgcag gcaatcaagg cgtgcggtct tccaagagga gattcttccc tgatgatgtt
    10621 gatgaggtgt tggcgcacca aacggctcac gctttgttct cccctcaaac tgattcctcc
    10681 ctgatgccat agctcaccaa tcagtcgcaa gaaaggaaag ataatgatag tcaaagcccg
    10741 ttccagcccc agaccatcaa tccagttttc aagtatggtg gtgattccct gcacatcctg
    10801 ccgccgcgcg gatcgaatca tttcctgtgc gtcattccaa tatgtggagg gtatccctga
    10861 gagatcaagg gggacatgct gcatcaactg tcggagttcg tgctcaccgg cctgggctaa
    10921 ttgaccaatg gactcaccat gatccacctg tgatttcagg tacaggagaa attgaatatc
    10981 gtcttcagta aaaagtcgat agccgttaga agctcgctga ggatggacca attggtagcg
    11041 ttcttcccat ttccgaatca cgtgggcgct gagtcctgtc gtactggcca cagatttaat
    11101 gcgaaatttc tgggaatcat ttgcaatgtt agacatgatt gcgcaacatc agctctttcg
    11161 gatgtggatt gagataaacc tgatccatta aatagggaac agccaggatg cgcacataat
    11221 gtttaatgag cgtcagagga acagccaggg gtgtgagatg ccggtggtaa tcctgaatgg
    11281 tttcctgcaa ctcggcccgt tcaatggtgc tcaattgttt tttgaagtag ccggccagat
    11341 gctggaggac attgacatgc tttcgcacgg tggccttgat tttcagcgcg tccataaata
    11401 atggaccgta tcggttggcc agatcaccag ctgtgtattg gcctgcctgc gcgatgagtt
    11461 gtccaagttc gtgataatgt gaacggctgt gggtgagcaa taaatatttg tggcgggtat
    11521 ggaattgcac aatggcccct cggctgatcc ggtgctcctg aaataaggtt ttccaccttc
    11581 gataacagaa aatgcgctcg ataaagtttt cccgaagggg ggcatcgctc aaacgacctt
    11641 cctcctccac aggaagcagg ggaaacgcgt gttggaatgc ggaggcaaag atacccgttc
    11701 cctgcttgga aggttgacct ttatcggtat acaccttgac ccgatacaca ccgcagcttg
    11761 gggaactttt tttaaaaata tacccatcca aatcactttc ctgtaactcc tgaacccggc
    11821 gctgactaaa gtcgtgcagg gtggcggtgt gatctatgtg ggagcggatg gtcaggagtt
    11881 gtggagcctc aggatcgccg gccaaatgca tggcttctcg cggagtactc agtccggctt
    11941 ccacttcagg gcagaccgga agccactcga cgaacggggc taggacgtct gtaagaaagg
    12001 catcacgttt gtgcccacca tcgtatcgaa cggtatcacc gaggaggcat tggctgatcc
    12061 caagtcgcgg cttatgatca gaaaggggct cagccatatt gttgtcgggt cgggtcgttt
    12121 ttttgtgagg tttgtgatcg atgtcgataa gtggtgtggt cataggggtg tgtaatccct
    12181 ttcctgacgg gtgacggatc caccatgccc atgtcgcatg tttctcaatc cttctccggc
    12241 gttgagtgcc tattattcat gacaagtctc ccccaagggt ttggcgttcc tgaatccttc
    12301 ctaacttcat tcaacccctc ggattcggtt tcccagggaa gatggcctgc cctgcaactt
    12361 ttactccatt tacatggctt gaggtgtttg ctccattaac accgattcgt taattctcac
    12421 gacgctgacg gtgcgttcac cttgcgggcc tgtaagtccc agtcggacgg tgcttcccac
    12481 ttctccacgt ataagcccaa ccgcctcctg ataggttttc ccggctaggg ggagcccgtc
    12541 gactgtcaga atttcctccc catgattcag tccagcgcgt gcggcgggtc cggacggatg
    12601 cacggcgcga atgatcaatc gagcaggttc tccaattcga accgctgtaa tatgcagggc
    12661 aagtccgaca attcccgagg gagcgggtgt gttggcgcct ggagggctgg cgtgggggtt
    12721 gctggaagtt gggggttttt catctgcctc ttcgtaagcg agacttccgg gagcgaatga
    12781 gccaaccagg atcaaagcca tgacgaaaca gggaagcatg aatctgctta cgcgattgtt
    12841 gaaaggtgca tagaggcgca tatctcaatt ctccttttat aaattacggc gagatgccga
    12901 ataagcccat gaggcacaaa cccatgaata agaccaggga attcagttgt acggaaaggc
    12961 ggtgaagacc atcccgttgt tgttgaagta acggggttgt gaggcctcta gcctgcttgg
    13021 ctttcaattg gtcactgagg tcattgatct gagcaatgag gggtgtccgg cagtaatgtt
    13081 caatggccag aacgcctaac cagattgtgg tgggaagcaa gacgatggat tcaaatccat
    13141 tcagcagtcc cagggcaatg ctggtggtcc agccaaccgc agcactgatc attcccaatc
    13201 cataatactg aggaaacagg cttcggacga ctttggcgaa ggtgtcggta tccagggtcc
    13261 gtgagagaac cggcgcggtc acaaacgaca gaaagacaat cttgcccacc aaaatggcca
    13321 tggccaacaa attgaaaaaa ataagaatgt gttcagtcat cgtgtcaccc ccttaagaag
    13381 cattgggcag caggctttgg ggcacccgga aggcctggga aaaatcaggt aactggtcca
    13441 taggaactgt ttcaacatat cgaatactgc ccttggcatc cagtatgagc atcgcgccgg
    13501 agaatgctca agtcttccag aagaagccct gtctgtgtgc cgaaccgata ttcaagaacg
    13561 tccgctaaaa atggtatatg atgaatattc gccttagcag aaaattcctg ttgatcgttg
    13621 taggcaatcg tactcaaggt gacgaacgtc atcaggtgat ccaatcctcc attgcgttcg
    13681 ctgaatcggt gtgtctgttc atcgcacacc ggcgtattca actatggtac gacgttgatt
    13741 aacgtcatgt tccccctgag ggtatcgagg gcaacggtcc ctccattttc tccgatggcg
    13801 ctcgcagagg gaatgagatt gcctttctcc agggagggcg atgctcccag aacagaggat
    13861 tcccagaaaa aagaacgact ccgacagtaa aaattgaaaa gcacagcatg aagaagatga
    13921 ccgaaagtga ttgttttttt gcctctaata ttcatcggtt cgctcattag attctatggg
    13981 aatagtcccg ctacgtccct tgagttcatg atttgcccaa tattgattgt aatgctttct
    14041 gtagggtggg aaaggaatat gaaaatccgc tatccagggc cttttgcggg tgaacgcgtt
    14101 gtccggttgt cagcattgtc gcgagctctc ccagagcgat cttcaacgca aatccaggaa
    14161 ccggcaacca ggaaggacga ttcatcgttt ttcccaatgt cgtacaaaag tcctgcatgg
    14221 tcactgcttc cggagcgacg ccgttaaccg tcccatcgag agtggggtgg gtgatgacaa
    14281 aaaggataag gcgagcaaga tcattccgat ggatccacga aacatattga gtgccgggta
    14341 gaacgggacc gcccagaaac acgcgaaacg gcacagtcat ttttgataga gctccgccat
    14401 ccggtcccaa gaccattccg aatcggactg gaatcacccg aaggtccaag gcttcggcct
    14461 ccatggccgt attttcccaa acctgagaga gatctgaaag aaagccctta ccacgcgtag
    14521 aagattcatt cagctcttcg tcacccctgg ctccatagaa gccgactcct gaggcggaaa
    14581 taaatgtgtg aggtttgtgt ttccaggttg gtatggccag aagcaaggct tggatcggtt
    14641 ccacccggct gtcaatgagc acttgcttcc gcttgtcggt ccagcgggaa tcggcaatcg
    14701 gtgcaccgga aagattgatg acaacatctg caccctcaca ttcctgcgcc catggtccca
    14761 gagtgactcc atcccactgg acgaaatgac gttgtggggt ggcagacgca gtgtaatttt
    14821 ctgcatggcg ggtgagaatg atgacggaat gtccttcttc aatcaacatc cggcaaacct
    14881 ctcgtccgat aaatccagtt ccaccggcaa tgaccatctt catgttcagt ctcctatctc
    14941 atgatttcag agggggcatc ccattcttgc aattccaccg taggaacaat atgagcatct
    15001 cccaccagat caatgccaaa aggatcgggc ttatgcgtat cttcagggta ggcagttgtc
    15061 gaagttgagg aaacatccac gtccacgttg agggtgcgtc cgggaaggat gtgaatggac
    15121 gtgatggtct gacccatcgg gtgccagatg acgagattgt aggttcccgg cggaatgtca
    15181 gtgatggaaa attcaccttg ttttcccgcc tgagtgacat agggatgtgg aagtacgata
    15241 ccccaccctt ccataaattc atgatagcta cagcgaaaga ggataatgcc tgtcttctcc
    15301 accgcatacg tgactttcgt cccggctcga tgggtgccgg tcttgccttc tgaaagaaaa
    15361 tcactcttac gattatcggg atgcggtgga agagtctgcc cgaacagatg aagtccaccc
    15421 gtggcagagg ttagaaaaat ttcgagtttg tgttcaacag gatcccaatt ttgaaaatgg
    15481 aggtcctgac cggcttttgc ggtgccgaca tgaggaagaa acatacagtc tttggtttga
    15541 accatggccg gtgtcgaggg agcggctttt cctcggggga cgttctccag atagaccacg
    15601 gcaccaggca gggagtggtt tggacccggt ctggtgatgg gagacaaccg ccaacctttt
    15661 ccgtcagaaa ttcgtccaca ataataggga tcggctgaga gaacgaggtt gtaccgtctt
    15721 ggtgaatgaa gtgttccctt catgaagacg gatcccttaa gatccccccc agcgaaattt
    15781 gggatttctt cataagcgaa tcctgaatgc acaggattca gcaccgcaca aaaagtcacg
    15841 gcgaagaccg cgagtatggt tcctctagcg ggaaagaggg gctctgttct caaaatcaac
    15901 atgattgtct gcctgtcgat atcccatgaa tcgaatgatg ttctatgcct ctaccttatt
    15961 caaccggaaa ggagatgata attcctccca tgacgtcacc cagtttccga tcacgttttg
    16021 ggctgaggag gtgtgtgtta tgacaagtga cgcaggccct ggagattgcc cggtctgcat
    16081 agatcgcctg gaaatatgtt tgttccccct tggtgatgta acctgtggcc ggttgagacg
    16141 gctcacgttc tacggccgcc agaccagctt cttcgaagtc cgaggcagga ccattttttt
    16201 ggtaaatagg cagacggctt gccagacgat actcaaggcc gatgcctttc tccttcacgg
    16261 cagatcccga cagcagcagc atttgagccg gaaggggcag agcgttccgg gactcccagg
    16321 tttccgaggc gatgaccgtt cctgtttcct gcatgcggtc gacgatatgg gtggtataca
    16381 gggtccggtc agattcgatc accgcataaa tgaaatctgc cacgatttcc ggtcgaacgc
    16441 ctttgatcga atgagtgcct tcttcgctag cagcgatgtc tgacatagaa aagcccaaga
    16501 ggatcaccgc tgaagttaaa cagaggactt ggagcgtttt catcacgaca cctttatgat
    16561 tcattactgt gtattgcttt ctgactcggt ctgacccaga ccgatgtccc gcgcacatcg
    16621 aaacccgatg ccatgagttt ttagtgcaaa tccacccttg cctcgggagg tggctctcaa
    16681 atcagtatca aaacttttcc acgatcctcc acggagaact tttaaaattc cggtctccgg
    16741 gcctttggga tttttgtccg gagcgctggc atagtattcg gaggcatacc agtcctgcac
    16801 ccattcacgg acattgccgg caaggtttcg gagtccgtat ggtgattggc cttccggaag
    16861 ggccgtcact tcgacaaagg tctttttctc ttcccagtct ctttcaaaat tggcttgttt
    16921 cggagtaggt gcttggtccc cccaggggta gagacgacca tcgtttcctc gagccgcttt
    16981 ctcccattcg gcttcggtcg gcagacgctt tccggcccaa aagcaataat cgacggcatc
    17041 atgccaggtg acctggacga ctgggagttc cgcaatgccc tcttccttgt tgaacgggcg
    17101 ttcggcgaac acattcattg gcggtttatg taacgtttca tccaggaatt tcatgtaatg
    17161 cgcattgctc acttcatacg tatcgatggc aaaggcattg aggtaaatct tgcggcgagg
    17221 ctgttcgtcg aggcgaccct ctcctggggc gcttcctcgt atgaacggcc cggccggaat
    17281 agtcatcatt tcgaccgggt ccttttctat cgtggtcgct tccgctgccg tggcagtcgg
    17341 tggggtaact gtcagcatga gtgcgattat cattaaacac cacatcttca ttgtttcttc
    17401 tcccgacttg cctgaacaat gttctgagtg atctgcatga tcttttgtgt gagagtgttt
    17461 acggcatcaa attggttgtc ccgctcagcc tttctggcct ccatcaacaa cgcgcgggct
    17521 tcgtgtaact gtcgttcaat gctggactga actgctggtg aagccagggt tcctccgatg
    17581 gccgcagcat gaaactcctc ccaggcttgt tcggtggcct gggaggcttg ataaataagg
    17641 ccccggctcc cttcctgctc ctgtgccgcc actgggggaa ctccgtagac cggagttcca
    17701 ccctggatca tgggaatgca ggagaggaag aggattcctg ccagataaga ggaggccact
    17761 cgcatgttca ttactattat attccttgct tgattgaccc tcatgggaat gttcctaccg
    17821 aatattgtct tgaacgatcg cctgcatctg ttgaagttct gtattatgag gatcgagttc
    17881 aagggcggtg gcaatttgtg tcttggcctc ctgccatttt tcttgtccca ttctcttcat
    17941 ggcgtcaatc gtagccgttc gtactttgtc attgactacc gtggaagcgg attttgcgca
    18001 tcggaatccc agtcctaccc cgtagctgtt attggatgga tgattccaaa accgatgtgt
    18061 ggtggtgatg ctggattgtg cggcaatcca tgatccaccc cgaatgaccc gcttctcacc
    18121 gacggtcagg cgatccacat atgtgccggt tccgccgatc cagatgggtt taagtggccc
    18181 catggtgttc accgaatggg gagtcttcct gtaaaaatcc gggtcatacc agtcttccac
    18241 ccattcaaaa acattcccag ccatatggtg aaggccgtac gggcttgccg cttccggcat
    18301 ggagtcaatc ggcagggtgg cagcatgatt tttgccaaag ttggcttttg ctgcatcgaa
    18361 ctcgttaccc cacgggtatt tcaatccctc agggcctcga gcggctcgct cccattcggc
    18421 ttctgtcggg agtcgcttgc ctttgaattc gcaataggcc ttggcatcaa accaattcac
    18481 tccaaccacc ggctggttgg gtttgttcaa tcgcgggtcg tcccaatagg ctggtccggg
    18541 atgccccttg gtcttcatga actctgcata gttgctgttt gaaacttcgt atttgtcaat
    18601 aaaataacca tcaaccacga cccaatgggc cggtccctca tcatgaaatg cctctttgga
    18661 ccagggcata ctcattctcc gttgatagag tgttgcagag tcgcccgtgg ttgacgcggc
    18721 cttagacgga tctttgtcga tgcccatgat ggaagggccg aacggcacgt acgacatttt
    18781 gtctggcacg accgcactgt cctgagcgaa tgctgccgat ggatgaaggg ttaacaggag
    18841 actgccgagg aggacgcctg ccacaggcag gcgtcctcct gatctggtca atttttcatt
    18901 tctgacttct ccagatttta tgtttgtcat aacgttcttc ccgtttagtt aaaggctgtc
    18961 gaataaatga tcaaggatca tgacgatcgg gacttccatg gtttactttt tgtctttatt
    19021 gatttgatgc atgatcaatg tttgggtctg cagatattct tcatttttcg gatcggcctt
    19081 gagagccttc tcaatggatg tgagagcttc ctgccatttt tcctggccca tgctgatcag
    19141 agagagcatg aagtcatatt ggacgttctg aagtcccgct ggagaggccg cctgggcaca
    19201 gcggaaacct aaaccaacgc cataagaatt gttctcaggt tggttccaga atcgatggct
    19261 ggtatgaagg gagctagccg gtgctaacca ggagccccct tttaacactt tgacctgacc
    19321 ctgattcgca aaattgagac ctgtggaagg accttggggg ttcatggcag ggctgacgtt
    19381 aaaataaccg ggatcatacc aatctgacac ccattcaaaa acgttgccgg ccatgttata
    19441 agccccgaat ccactcctac cttctggata ggagtccaca gcggttgtct gcccgacggt
    19501 ttgaccatag ttggccttgg aagcatcaag ctcatgtccc caggggtaat gggcatgtcc
    19561 atcaggccct ttcgccgctc gctcccattc cgcttctgtc ggcaatcgtt tccctttcca
    19621 ttcgcagaag gcgctgctgt cataccaatt gactcccacc accggttgct cgggtttgtt
    19681 aagtcgtgga tcatcccaat aggccggagc tgcgtgtcct gtgttcttca taaattcctt
    19741 gtaatctttg ttggacacct catacgtgtc gatgtggtag gcatcgagga ccacgttgtg
    19801 aggtttctca tcctgatgat tttcactgcc catcacaaat tcacctttag gcacgaggac
    19861 catattttca ggaccggcgc tcaatgcagg agtagtgatg aggagtagtg aggccatcat
    19921 ggtgccaccg atggtctgat gaaacggctt catagagaaa ttcttccgat agagtgaatg
    19981 ggagattgag aagttccgtg gtacattcat gacgataacc ctccggttaa ttgtttgagt
    20041 gatggtgaaa gagatgccgg ttttttaaaa ccgtggcatc aaatagatct ccgcattgca
    20101 ggcatttcca cacggggatc tgaaattcag aggttccttg atcgggagag atgcaataac
    20161 tactcaccaa gaaccctcca cagcgcgaac aggcatgaag caatcttcca gcattttcag
    20221 tttgcctcct tcccgtttgc tgattggtgt gttttgtggt gtgtgtcatt gtagtccctc
    20281 cgttttaatt taaaattaga caaatgttgg tttgactgat gtttgtctaa tgtatgttca
    20341 tagttataac aatgtgttta atgtttgtca attgttttaa aaaaaagatt tgacatacat
    20401 tatacgcatt gatataaata cctaatgcca atgtatagaa ttcatcgttt ttccaagctt
    20461 accggccttt ctccccacgt catacgagcg tgggagaggc gatatgggct ggtgaaccct
    20521 gttcgaggcg ataatcgtta tcgcctgtat acggatgacg atgtggtgtt gtttcgatat
    20581 ctcaagtctc agatagacca gggggtgtcg attggggaat tgtccgtgca gggacgtgag
    20641 catttacagg agcaggctca gcgggtattt gtggaaaccc atcgtgagcc gccaccgtct
    20701 gaacgtttgg ttaccgattt atcacaggca ttagaggaga atgatcgtgt gggttttgaa
    20761 aggaaattga atggcgcctt agccgtgatt ccttttgaag aagcgttaca tcgtttttta
    20821 ctccccttac aagaacgggt tggacaactc tggcatgatg gaaagcttgg cgtgggccag
    20881 gaacattatg cgtctaatca agtcaaacaa aaaatttttt cagccttgaa ccattttcaa
    20941 acttcagatg tgggacctgt ggtagtcatt gcctgtccta cgaatgaatg gcatgaagtt
    21001 gcggcaatga ccgcagccta tgtctgccgg gcacggggtt gtcgagtaca ttatttgggg
    21061 gccaatttgc cgattcctga attagcggcc tattgtgacc gtaaccgtcc cacctgtgtg
    21121 ttgctttctg tgacaatgat ggagtcttca gaggaagcca tcgccctggc acaagaaata
    21181 gccggtacga ttgtcccgct cactcctgtc ggtttagggg gccaggccat ccaagcttat
    21241 gctgaggcat ttttgcagga aaatatcatc gtctttcagg acttgaaggc tcttgatcgt
    21301 tttgtggttt ctctcaagca ctaagccttc ctccatttgt tctagagaat tttttccctt
    21361 ccgatattcc ctgtttcata tttgagaaag ctttgatatg gtgccctttt ccgccattca
    21421 gaggcgatag aattcgaatg gcatgttcct gaatcaaggc tttcaaaatg agcagagctc
    21481 tttgcctgag acaatgattt gagggaggct gcagggttat ggaaaacgac ttgtatcaaa
    21541 ttatctatgc cagtgcagca acacggcgaa ttgaagaaga cgagctactg tcgatgttga
    21601 aacacgcacg taataaaaac aagcaacttg gaatcacggg tatgttactt cattgtgatg
    21661 gatcatttat tcaggcactt gaggggccca aagaccaggt gatgaatatg cttggggtca
    21721 ttcgacagga ttctcgccac aatcgaattg tggtgttatt tcaaggtccg atcaagaacc
    21781 ggagtttttc ccaatggtca atggggttca acagaccttc ccaagaaaaa ctggcaacga
    21841 gcgaggggta taccccctat cttgacgtag gcgatgattc ccaggccatt aacaattact
    21901 ccagtgtcgc cctaaaatta attggcgcgt ttcgtgaact atctgataaa caatcttcat
    21961 ccatggaggg ctaactccat gctcacctgc atattcttcg ggaacataga ccggacccta
    22021 cctatttcga aaacactgct gtgaaaggac gggttcccgc ccataacaca cggggtgctt
    22081 tgtcttgtgc gaatgacttt tttgcaacga tgtcgttact cttcaatttt ctaagacatg
    22141 tcggtggacc ctctaccctc aaagacatgc cctttcctct ctgagcaata cgtggaagtg
    22201 tccctgattt aaaatgtctg gagcctcatg tgttctctcc tcccccttca tgcatgttta
    22261 aatggctttt gtgatcaaaa atgaatctcg gtccgaggta tcgcatgggt tgagatgtgc
    22321 tggaataaag gaatgtgtga cttctgaagc aaggtctacc atgttgagac atcaagaaat
    22381 tgcgccaaca cccgaaagaa caatatgcct ctcccggtcc tctgctctga agattctcca
    22441 cctcacgctc caccccggtt gaaaccctgg cggcacgcat ggtcggcggt cctgatcgtt
    22501 ggatgtttct atgttgttca ggcccttggg atcttcatgg tttccgctgt gggcggggcg
    22561 gtggagacgt tggccggggg aattgacgtc ggggcgcagt ttggcacggt acctcagtat
    22621 aaggaagttt ggttgcttcc cctgtcgtta tgtgtgggga cattgggagc catgatggtg
    22681 tcgatagacc tggcgagacg gcgagcggtc ctatcttcga atgcaaatgg gttcagtgag
    22741 ctacttggga aatcctatga ttttcgaaat ctgtggcgtt ttgttttgat gggattagcc
    22801 ttgggttttg gatttgttgc ctttactgaa tatggtgggc tcccagcgga cgatcttcct
    22861 caaccgctct ttgatgccat gatcagtgcg cctccttcgt tacagacttt atgggctttg
    22921 atgattgttg gggtatttcc tgttgtggaa gaggtgctct tccgggggat tttgtttacc
    22981 ggaatgtcgg aatcctgggg accgggtttg gctggtttca tgaccacgat gatctttgtt
    23041 ggagtgcata tgccgaaagt gcttgagtat tggccggcac tattagccgt ttcattggtt
    23101 ggttcgttga cgatcctgtt acgcattcga acagaaagtc ttgtgccagg aatcgcgctc
    23161 catggcacat ataacggcgt tttagtcatg gtagcgtttc tcacgcaagc cacttcgtag
    23221 tgcatccggt tttcgtgggg gtttcgttgt aagctttttc ctaaactgat tggataggtc
    23281 tttagccctg attgacaggg gctgacatgt ctgctaaata atttactagc aacaatgaag
    23341 ttgctggtca tttagcaaag atgtgttttt ggacaatgat cgtccaattc cttttcaagg
    23401 gattgggcgt ttttttttgc cgaatgcgaa acatggggtg atgggttcag ggaactgacg
    23461 tattgatccg acaatcttcc gaaacgaacg gtgtaagaat tcggtaggaa caaagaaatc
    23521 agacttcatc atttcgagga gtgaatggtg ggggaagtgg caaaggcgcc actgtttggg
    23581 caaggcggat taatccggcc ttagaccagg cagttggcgc ttttttatta tgcggattac
    23641 ggaataacag aataaggaga gagtcatgca tacgggggat gtcgaacgaa tggttcagct
    23701 ggcagagaag aaagtggggt acttccgaag taatcccatt gggtacctgg tcttatccgc
    23761 cctggcaggc atttatctcg gttttgggat atgtttgatt ttcagtgtgg gcgctccatt
    23821 ttgggccgaa ggatcagccg ggtttaaatt agtgatgggc gtgtcctttg gcattgcgct
    23881 gacgcttgtt atctttgcgg gttcggaact gtttaccgga aataatatgg tgtgtgctct
    23941 tggtgctttg gccaaggcca tcacgatgaa acaggtcggt cagatttttt tctggtcgtt
    24001 tgtgggtaac ctcgcgggat caatggggtt ggcatggttg gtcgtacaat ctggggtggt
    24061 gggacaggcg cctcaaagcg atctgctgct caaggtggcc gcgcttaaaa tgaatcttcc
    24121 gatgtgggag ctgtttatac gcggggttct ttgtaactgg ctcgtctgtt tggcagtgtg
    24181 gacggcatcc cgcacgacca atgatgtcgc gaagatcatg ctgatttttt ggtgtctctt
    24241 cgcattcatc ggaagcgggt ttgagcatag cattgccaac caatcgctct tgggcattgc
    24301 cttgtttctc ccgcatggcc cggatatatc ctggaccggt tttttgtgga atcagttgtg
    24361 ggtcgtatcg ggaaatatcg tcggtggtgt cgtatttatg ggaggagcct attggatcat
    24421 cagtcctgtg cgtgggtgga tcaagaaagc agaagccgta gcggaaggtg cgtcacttga
    24481 atcgtcacgg aggattcctg taccagcgga gagggagtta ttgtctcagc ccctggcggt
    24541 tgggcgggag aattaggaac tagagcaggt cgtgtgactg aatcgagaaa gaaacgtgaa
    24601 aggattctga tgaataagat tgaggtgctc aaaggcgaaa aagacgggtt ggacatcaaa
    24661 gaggatattg cccgatatgc tgaatcgggg tgggaggcga tcccggatga agatattcaa
    24721 cggctgaaat ggtatggact attccttcga aatcccacac cggggttttt tatgatccgg
    24781 gtccgtattc caggaggaag aaccacttcg gtccaattac gcacattggc gcatctggcc
    24841 aacacctatg gaaatggcgt gttggatctg acgacccgtc agcagtttca gttgcggcat
    24901 ctgaagatcg agcatgtccc ggaagtcttt cgtcaaatgg aagaggcggg cctttgttcg
    24961 ttgcaaaccg gcatggacaa tgtccgcaat atcatgacct gcccggtggc cgggttaacc
    25021 acgcaagaac aactcgatgc aacagacttg gtgaaacggc tgacagaagc actggtggga
    25081 catcgggctt attcgaatct tcctcgaaag tttaatttgg ccatcaccgg ttgtctcgac
    25141 aattgtttac acctcgaaac tcaagatctg gcactggttc cggcaatgct ggagggacgg
    25201 gacggagcgg tcatcgggtt taacctgtta gcggggggga aactcggctc tggcgggtat
    25261 cgcattgcca ctccgctaga tgtattcgtc agcccccagg aagtggtggc cgtgaccgga
    25321 gccatccttc gcgtctttcg gaatcatgga tgccgggact cgcgcaccac tgccagactg
    25381 gcttttttat tggatgactg gggtgaagaa cggtttcgca tggaagttca gcgggaagtg
    25441 ggatgggaat tatcgcgagc aggaaccgat tcacggaagc actccgtcaa tgatcatatg
    25501 ggggtctacc ggcaaaagca gcc
//
LOCUS       DAORAQ010000029        25200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_373_length_25200_cov_65.336210, whole genome shotgun sequence.
ACCESSION   DAORAQ010000029 DAORAQ010000000
VERSION     DAORAQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 25200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25200
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_25200_cov_65.336210"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PKM72_04625"
     CDS             complement(<1..1048)
                     /locus_tag="PKM72_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNP60102.1"
                     /translation="MIVGVLKETYPGEHRVALVPAVLPSLKKGGMDVIVESEAGEQAG
                     YPDSTYIEKGATIASSRAQVFEKADCLVQVRLLGANPTEGQSDLASFRKGQMLIGMAE
                     ALTVPQSVQDLASREVTAFALELMPRITRAQSMDILSSMGTVAGYKAVLIAASSLPKM
                     FPMLMTAAGTVTPAKVLIIGAGVAGLQAISVAKRLGAAVEAYDIRPAVKEQILSLGAK
                     FVELPLETGAAEDKGGYAKAQDETFYKKQRELLGKVIAASDVVISTAAVPGKKAPILI
                     TTDMVGAMAPGSVIVDLAAERGGNCELTKPGHTIVVDGVTIHGPENLSSTVPYHASQM
                     YAKNVATFLLHLVKK"
     gene            complement(1310..2584)
                     /locus_tag="PKM72_04630"
     CDS             complement(1310..2584)
                     /locus_tag="PKM72_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60103.1"
                     /translation="MPHENNFQHANYSKSDPGARVGYRTFQPGAAPDSPEWGQAMAGV
                     GEHMAQSRVKGVLFLQGLPFMDLFGAARLDEVGGLKRGYSRGISGLESLLALLRPATN
                     GICFPGDSLHPPVSDDEATHKRLDLLAQEIGNFSSSYVRNIELALTPASEKSIPCNRY
                     IWSSVNHHVGRVEAAIHLLMYLQKWGSSLDLTKDDRVLLVGHGHAGQVLALLSNILTK
                     GESEARARVFEILAKYWQSCPSADRSVAQLERMYGLVMDQVALNGAAVDVVTLGTPIR
                     YGWDIDGVGHLLHFVNHREIRTDGKRWLAKMELPQIAWELPYQTGGDYVQQLAVAGTD
                     MVPNNPEAEQANVDFREIFEPYDGFERWLECTRRATRCANDGQCLLVEYGVQAEESPR
                     QHLFGHACYTHSRAMLFLATEIAQAFYSKKSS"
     gene            2847..3890
                     /locus_tag="PKM72_04635"
     CDS             2847..3890
                     /locus_tag="PKM72_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNP60104.1"
                     /translation="MSTSQIPTPQHRRGKRMRCFFFAWSLVWLPLIGGTASIASATQA
                     EHIILVVLEGIKSSSIQSGATPNLARLAKEGVVTWAAHSIAPPLTVPAMASLLTGLQV
                     EKHRVTAEWETYDFARSFMRSPTVFDYMDLAGGKDTGVFLMDERLYQLIRPEIYVDSQ
                     VCGYTKPQCTPERASAYIKDFLKKVTSEKGYGFRLFGVPNLLLVHLPTAVKVGQKYGW
                     DSEKYSDALKAIDTTVEQIIQTYKELGVLDKTMVIVTGLNSGPLSSPVGGVKPVSTSP
                     TSPELDATVPWIAWGANIKKNHPITQSVSLLDTGATIMYALGLETYTEWDSHAIGEIF
                     QTVPERRTTGNEP"
     gene            3880..4353
                     /locus_tag="PKM72_04640"
     CDS             3880..4353
                     /locus_tag="PKM72_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60105.1"
                     /translation="MNHKTNNKTMIRILFPRQFTHIVFCLLLMGISPLLIQLGHTADS
                     NNPLLRQFPITVNPSALAPPTWWHVPGMTPLIWTKDPISSDAYKTSETRTLNLKPGEY
                     RFGTFTFDFLFKVTLSGTLEFSPTLDQCVDGRGTQVLTIRCSHTQPYPQEPDYPK"
     gene            4378..5766
                     /locus_tag="PKM72_04645"
     CDS             4378..5766
                     /locus_tag="PKM72_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP60106.1"
                     /translation="MSNSVQDWIDALEDADDATREEAAKALAEKGDPKTLEPLLNALE
                     DDYWSVRSHVGWALAKIGGEKAVEGLITLFNDNMMEVQAEAVNAMASMGKSVIPQLLT
                     CLKDKRWRVREQAAKTLGELRDPQAVQGLSLVCRDRDGAVKSAAAEALGKIGDPKAIP
                     TLIKLFKDTSKIVRETAGTALMYVGKESVDALLESLKDPHFVVRCHAVRALGGMTTDY
                     QMGRVWVREPRVVDALIDMVKDPDRAVREDATIALGNIGDARAVDALIEAMKDGTVKR
                     HAIASLGMIGDPRAYPPVLDALKGKGIHQEGKPTPGCIISEDLLIKEAAATALGHFRH
                     PKVIPDLVLLLKDLVLRDYAANSLVLIGDAAIEPLVWFFHDPDLSKVQQESERVLAFA
                     TSRMTAGGALKKTILDTLDKLGWKPSEGISKETREIEYTDTSNVLGEGGEGRFGKSGD
                     FAMPAKPPKIER"
     gene            complement(5831..7513)
                     /locus_tag="PKM72_04650"
     CDS             complement(5831..7513)
                     /locus_tag="PKM72_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP60107.1"
                     /translation="MTQKATMPVSKQKYGDNPIAQRKTDLYKQEYVHSFVQKWDALID
                     WDARASSEGDFFIRILKERGVKRVLDVATGTGFHSIRLMRAGFEVTSADGSPEMLARA
                     FENARRAGFIMRTIHADWRWLSRDIHNKYDAVICLGNSLTHLFSEQDRRKALAEFYTA
                     LKHDGVLILDQRNYDGILDNGFTSKHVYYYCGDNVSAEPEHVDEGLARFKYTFPDGEA
                     FHLNMFPLRKEYTRRLMNEVGFQQVKTYADFQETHKVEDPDFYVHVAEKRYKNEERPP
                     TIMGKIGNLNYSPTVDTARAYYNSSDADRFYATIWGGEDIHIGLYASDNDSIFDASRR
                     TVEKMAASVKGLDSTTRVLDIGSGYGGSARHLVRRYGCQVGCLNLSEVQNKRNRELNQ
                     KENISLAINVIDGNFESIPMPDGSVDLVWSQDAILHSGDREKVFQEVHRVLAKGGQFI
                     FTDPMKSATCSPDVLQPILDRIHLDSLGSIEYYRKLAAKIGFKEVGVEDLSANLASHY
                     ERVLQELTTQHHMLIRVCTEDYLANMKVGLQHWVEAGKSGHLSWGILHFRRD"
     gene            7939..9474
                     /locus_tag="PKM72_04655"
     CDS             7939..9474
                     /locus_tag="PKM72_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HNP60108.1"
                     /translation="MPSMVRPNLPIHFYRPMNPLKSTTLVFNISCALAFLFLLWGAFA
                     PKHLAKITGTVQTALLDSFGWLYVLAATGFLICAFCLIFSRWGDIPLGPDGAKPEFPL
                     LTWFAMLFCAGMGIGLVFWGVAEPISHFHDPPIDEGGTPQAARLALQYSFFHWGLHPW
                     GIYTMVGMALAYFQFRKGTPGLFSAGCQPVLGRHATGPMGTAVDVVAVFATVFGVATS
                     LGFGAVQISGGLSYVFAMPNTLTTQLAVIATVTVLFMLSAQTGLQRGIKYLSNLNMVL
                     ALTLLSFLLFLGPTHFIMTVFPSTFGNYIQNLPTMSLNLAPFRDSTWIHNWTLFYWAW
                     WIAWAPFVGTFIARISKGRTVRQFVLGVLLVPSIFCAFWFTVFGGTGIALELFQDVSL
                     ETVMEKQGIEVLLFTVLEQYPMGTVMSLIAIFLIGTFFITSADSATFVLGTLTTNGSL
                     NPPNRIKFTWGIIQSLSAAILLWSGGLKGLQTGAILAAFPFVFVIIILIISLLKSFQE
                     ETR"
     gene            9597..10046
                     /locus_tag="PKM72_04660"
     CDS             9597..10046
                     /locus_tag="PKM72_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60109.1"
                     /translation="MKLDSLPTEFTPYKQLEIATNKLVDGVALVSVNGFIPVLIGRGE
                     TPEIWVTIPADQQGKEWRPIVSKNSSLLDVITVTALGKKIEVAFADEIVLEVKEISGE
                     NAEITKCNLRPFGINVFLEDDCLKVMTNRYSGNTITEAEIFIDVGKG"
     gene            10578..10943
                     /locus_tag="PKM72_04665"
     CDS             10578..10943
                     /locus_tag="PKM72_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60110.1"
                     /translation="MTDLETPLPHITTTETLVRFYVLLSQYIDRCLDEATKRSLPEGE
                     FQKHLAETHTQVTELLATNRVVKNKAETEFQRITTLCEQFLKSPTDQVIKDNLNHEHD
                     VLRIKMLALSDLLAVFRSV"
     gene            11056..12183
                     /locus_tag="PKM72_04670"
     CDS             11056..12183
                     /locus_tag="PKM72_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP60111.1"
                     /translation="MVYIPPLGTNMVDSVAEHISALDDEDWGVREDAAVALGRLGDPR
                     SVQPLIRALRDSDRAVREAATSALKALGEPAILSLGFCLQDANLQVQESAACILADIA
                     NEQVLDPLLSAALSPNWIVRMSAAKGLSRIQNPQAIDTLILLLQDKVPAVREEAGRAI
                     QAIGNTSIPKLLEKLKDENWKIRLRAVEALALLKPLEAVGHLMILVLEDSDTAVRQDA
                     VRALGQIGDPRAIPLLLSSLALDTPSLKLPSIEALGQIGSTEAIPMLIALVNSLPKET
                     YEDRMEGCTDPQYKEDLPPLEAAIRALGKIRDPQAIPALIQALQSTLLRTEAAEALTQ
                     FGQKAVNPLLNLLKTTKNDNLRRHVLESLSHLGWRPGQIRI"
     gene            12199..13290
                     /locus_tag="PKM72_04675"
     CDS             12199..13290
                     /locus_tag="PKM72_04675"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP60112.1"
                     /translation="MPKETFETIISELDHEEDWRRMRATSTCMKGGPKAVEAIIQAMA
                     TGSTHYKVEAAKMLARLRDPRAGQALAHVLKDEDEQVRQSAVDALEHMAGILDEATAA
                     ALLEHLRDETLQEKVTELLGVIPTSIGPLSERLKDPDPAIRLRAAEILAHLLDPRSAN
                     AFVDAMSDPDLRDVATDTLRKLGAIRDRVDQTMDDLRAVDESELKEGVRQEAVIQLHR
                     IGRPIVEILIEYLEDDDWIVREAAADTLGKIGDIRAVEPLMVRLRSDSDTGVKEHALK
                     SLGLIGDPRPVELFIESIPIRPLRILAVEALEKVKEIEVLRPHMELFARLKTDRDGLI
                     SYNSGVILDKLEAATAQMSMDEEAWAEKE"
     gene            13306..14076
                     /locus_tag="PKM72_04680"
     CDS             13306..14076
                     /locus_tag="PKM72_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP60113.1"
                     /translation="MTEGNHVDPLLSALRDDNEALRNHAASRLGEAGSEAIPKLINMF
                     LEDDCVVREAATSALVQIGESAVDPLIEALKDDEEWSVREQAATALGKLRAIKSVEPL
                     VQALRHDKDGGVRTAAIWALERIGHPDAVPALVDALMDATLREDAARVLKKLGDSRAT
                     DALVEGLQASNWIVRRHAAEALGKIGDPRSLDPLIHALADEDWLVRRNAAESLARFKD
                     SRAIDPLVPLLEDENEMVRDTAEGALASLGWTPGSSRS"
     gene            14129..14797
                     /locus_tag="PKM72_04685"
     CDS             14129..14797
                     /locus_tag="PKM72_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP60114.1"
                     /translation="MANEAPAKLISIGPKSGTKKDGFNLVTENVTAINLDERQIEVEL
                     LAYDGKTVLMDVSEEAVEDLQKIKIGDGATLRVVEEDGKRIVKSFRIRSKDPNIQRAD
                     AALQDLTDSHWLNRKHAIEVLGELRVEAAVKPLVEMLSDEVGDVRQRAYEALIKIGAP
                     CVPDVIPFLVSEEDDLRQSATEILRKIGKPAVEPLALALGEADEKLQKRIMKVLDRMG
                     YKRK"
     gene            complement(14951..16282)
                     /locus_tag="PKM72_04690"
     CDS             complement(14951..16282)
                     /locus_tag="PKM72_04690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNP60115.1"
                     /translation="MYVSVIGTGYVGLVTGACFAEFGLNVLCMDTDASRIAALEKGKV
                     PFYEPGLSELVTKNLTAGRLRFTTDLHKAVDEALVIFIAVGTPSREDGSADLSFIDQV
                     AKDVGSRMTGYKVVVTKSTVPVGTAKRIRSIIQANQTTTCSFGVVSNPEFLREGSAIE
                     DFLRPNRVVIGADSPESVAIMKDLYRPLYILETPIVITNIPTAEMIKYASNVFLATKI
                     SFINEMANLCEGVGADVQLVAKGMGLDKRIGSKFLHAGPGYGGSCFGKDTAALINIGN
                     ENNYEMTIAKATKRVNDQQRVRMMEKIREAVGELRGKTIALLGLAFKPNTDDIRDSPA
                     LYLAEQIMKAGGTVRTYDPEALEISMKVLPTMIPCQDAYHTMEGADVVVLVTEWNQFR
                     SLDFDRVRAAVTSPIFIDLRNVYEPQRMEDLGFHYVSVGRKTVGAPPSQKS"
     gene            16435..16509
                     /locus_tag="PKM72_04695"
     tRNA            16435..16509
                     /locus_tag="PKM72_04695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16467..16469,aa:Gly,seq:gcc)
     gene            complement(16867..18174)
                     /locus_tag="PKM72_04700"
     CDS             complement(16867..18174)
                     /locus_tag="PKM72_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60116.1"
                     /translation="MDDQRLGLTAFRERIKISRESSPHHWEASRKFMVPETLQAKLPA
                     LIQHIQEASLDPTIQERLIAALQKFSQPIGSKEGNQILKDLTGFPPSKAVRALMVWGI
                     LNVNEERESPEVYSGIRWEEILRDVSNPYDVLRQTPTPSLLDIGAGDLSFEQELVDHY
                     VPYLRTQRTPLTLHAFDRLMPGSRVGGVYHKNLERERYLQGFSSEELRFKFWGGMGLE
                     TFSKSKGKLSRYTVSTCHAPANPTFAYEPSRLAPEIIHAHLQSSRGNYRKSRYEGEPI
                     LEVSHRGRTLTFPDWKFDILGPLALLKFMAQRSRVGILSAIDDGVFWEVLSQLLADDR
                     FRPRNEIFTNTVLPAIFGSVYEELTSMAVGQRKDLSVLADLRDSMPFGVVKNEGTQRP
                     PRFRYVEIRRGAVLGGVPSSFTARQFSEMKEESTPWWVIMVTD"
     gene            18308..19273
                     /locus_tag="PKM72_04705"
     CDS             18308..19273
                     /locus_tag="PKM72_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNP60117.1"
                     /translation="MVTHYFLRAKQHVLFLFLGIIVMSATAGCATSQANPTFGVNHIY
                     DVDRQQATTFDDVLPQLLAADVIYIGEEHYTPSHLEAAQKILTALLAHDRHPALAMEM
                     FSWDGQSGLDRYSQESEYTTEQLVKDSEWNTNWGGEFADYQPLVTFAKSHQLKIFGLN
                     PPRPLVRLVATKGLSEALQDPEMRKWPIQEIVTDDHAYEQLIFEQIEACHPGLPDHVY
                     RRIFEASIFRDEGMAQIITNYLNTRSRTAGPLVSYTGGGHIQYKTPVPKRVKRKHPGV
                     KSITIYLEAWDPSKEENVRSELQAGIADFLWLTPLGPKGLQPRCG"
     gene            19318..19563
                     /locus_tag="PKM72_04710"
     CDS             19318..19563
                     /locus_tag="PKM72_04710"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNP60118.1"
                     /translation="MGDPIIIAQRTPYVLNMEAGTYYWCRCGRSKTQPFCDGSHTGTD
                     ISPIEVELKEKQKVAWCGCKYSKKPPFCDGTHSRLES"
     gene            19803..19970
                     /locus_tag="PKM72_04715"
     CDS             19803..19970
                     /locus_tag="PKM72_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60119.1"
                     /translation="MNRITSLLSAFLLLAANPAFANEPGGGYEGITGLYYGTIALILA
                     YGVYDIFFKKS"
     gene            complement(20039..20836)
                     /locus_tag="PKM72_04720"
     CDS             complement(20039..20836)
                     /locus_tag="PKM72_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNP60120.1"
                     /translation="MVNNRDESYYPAIMHTCILSVLVLLCLGIWGCDPQESPSTGIPE
                     PVNHSSDTEISLAQFPPDQSSSELLVPKNESLPKIVAFGDSLTAGFGVSSDESYPAQL
                     EKQLHEGGFHYEVVNAGVSGETSAGGVRRVEWILKSRPTVVILELGVNDGLRGLSLEQ
                     TYLNLHSIIDRLLEEGVAVILAGMRIPPNYGEAYTGEFFGMYERLAQKLTLPLIPFFL
                     EGVAAQPGLNQADGIHPTAEGYTIVAQNVFRTLEPLLKTRRNHQAQE"
     gene            20944..21618
                     /locus_tag="PKM72_04725"
     CDS             20944..21618
                     /locus_tag="PKM72_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP60121.1"
                     /translation="MELQAANQSVKILQNISFEVLTNEVVAIVGPSGSGKSTLLGLLA
                     GLDRPTQGSIRINQTDITTLTETEMAHFRRKHIGYVFQAFHLLPTLTALENVALPLEL
                     QGISTGTVRAKALLQSVGLEHRLHHYPVQLSGGEQQRVALARAFIVRPPLLLADEPTG
                     NLDSSTGAMVIDLLWELHQQHGSTLILVTHDMSLAARAQRILALQDGAMVNETRPPLL
                     SSGSEA"
     gene            21615..>23646
                     /locus_tag="PKM72_04730"
     CDS             21615..>23646
                     /locus_tag="PKM72_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNP60122.1"
                     /translation="MTPVSVKLAWREIHSAWSRFLFLFLCIALGVGAIVAVDLFAVNV
                     EQVILGDTRALLGGDVELSWRRAISDKGRNVLDSLADRDMILSHVTEIAAMATVENPH
                     SQTASQLVELKAIDSAYPLYGQLVVEPDLPMTQLLDPLQSGCGRSPCFGALVQESLLI
                     RLNLTVGSELQIGKASFLITAVLKKEPDRIANGFSLGPRVMISRDALKATTLIQTGTR
                     IQERYRLSLSDSTSLEPLMGELRGRLSQEGVRVSSFRDAQPRLRRFLDQLNLYLGLIG
                     FTILLVGGIGVACTIQGFLTQKIPIIATLKTLGADSSQIIRLYLTQSLILGGIGSLIG
                     VIVGVILHRGLPLLLQGIIPETMPLNTSLAPVLRGIFLGTLATLAFSLWPLLAIRHVS
                     PALVYRQAVDHSQDMANSQSRLTRWRTILKRWWNDRAQVMVSISMMVGVTGLAMWQAH
                     SLTLGLFFSGACAIAVLLLLVGTGALYRILGHVPIPQWYLLPHAVKNLQRPGNFTKAM
                     TLAIGIGVMLMTTLTIVQRSLLDLIGNQIPSQAPSFFFIDIQPDQYPQFVDVLQQKFP
                     DSPYKLVPVVRSRLTTINGQPIDPEEHKGQRNGWYFTREYALTTSQDLPKDNVLTQGQ
                     WWDHTKESDSDGAPGRPLDIPLVSVEEDAAKNLGLTLGSTLTLDIQGVPIVTK"
     assembly_gap    23647..23746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23747..24412
                     /locus_tag="PKM72_04735"
     CDS             <23747..24412
                     /locus_tag="PKM72_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNP60123.1"
                     /translation="TLGSTLTLDIQGVPIVTKVGSLRQVDWGSFSMNFFMILQPGSFD
                     GAPFTYIATTRVPTNLEVPLQQAIVAVMPNVTAINVGDVLQNIGRIFHQLAMGIQALA
                     LLCLVTGAVVMVAAISINRYRRLHELAIVKALGASRRLLVFSLGVEFGVIGAFAGLVG
                     LGLGSLLSWSLLYLFFDLTWNFDLIVLSTGLLLTILLSLMTGFLGTYRLLGFPPLSVL
                     RQE"
     gene            25013..>25200
                     /locus_tag="PKM72_04740"
     CDS             25013..>25200
                     /locus_tag="PKM72_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60124.1"
                     /translation="MAKNILIIDDDEMSRGLLRMVLEYDGCECVEAENGASGLTLLES
                     KSFDVVILDNAMPVMTGM"
BASE COUNT         6433 a         6700 c         5749 g         6218 t
ORIGIN      
        1 cttttttcac taaatggagg agaaaggtcg ccacattttt ggcatacatt tgactagcat
       61 gatacggaac ggttgaggac aagttttcag gaccatgaat agtcacacca tccactacaa
      121 tagtatggcc aggcttggtc agctcgcagt tcccgccccg ctctgcagcc aaatcaacaa
      181 tcactgaccc tggagccata gccccaacca tatccgtcgt aataagaatc ggtgcttttt
      241 tcccaggcac ggctgccgta gagatcacca catcactggc ggcaatgact tttcccaata
      301 actctcgctg ctttttgtaa aaggtttcat cttgggcttt ggcatacccc cctttatctt
      361 ccgccgcgcc cgtttccaat ggcaactcaa cgaacttggc acctaaactt aaaatctgtt
      421 ccttgacggc aggccgaata tcatacgcct caacagcagc ccctaatcgc ttggccacag
      481 aaatggcttg caatcctgca accccagcgc caatgatcaa gaccttcgca ggggtgaccg
      541 ttccggctgc ggtcatgagc attggaaaca tttttggcag agaactggcg gcaatcagta
      601 ccgctttata accagcgact gtgcccatgg aggaaaggat gtccatgctt tgggcacggg
      661 taatgcgagg catcaattct aatgcaaagg ccgtgacctc acgagaggca aggtcttgaa
      721 ccgattgggg gacggtcaag gcttcggcca ttccaattag catctgtcct ttgcgaaagc
      781 tggctaagtc tgattggcct tcggtagggt tagcccccag taaacgcacc tgaaccaggc
      841 aatcagcctt ttcaaacact tgtgcgcgag aggaggctat agttgcccct ttttcgatat
      901 aggtggaatc agggtaaccg gcttgctcac ctgcctcgga ttccacaatg acatccattc
      961 cccctttttt cagtgaggga agcacggctg gaactagagc aacacggtgt tcaccaggat
     1021 acgtttcttt caggacgcca acaatcatag gggcatatct ctaaaaataa gggttataga
     1081 aattttacaa atttattaaa tctgcaggga attttaaggc tccttaacaa accctgtgtt
     1141 gatatcatag ggattcaaag agaatcaatg tgtctttgga tctataaggt gaatgagact
     1201 aacggcccat cgtgttaggc aagaccgagg caacacgcct catagccaat caacagagaa
     1261 atcacaggag aggtagccac gggttccgcg accaaaagaa acttttcact caactacttt
     1321 tctttgaata gaatgcctgc gcgatctcag tcgccagaaa tagcatggca cggctatggg
     1381 tatagcatgc atggccgaat aaatgttgtc gaggagactc ctccgcttgc accccatatt
     1441 ccaccaacaa acattgtccg tcattggcac atcgtgtcgc acgcctggtg cattccagcc
     1501 accgttcaaa gccgtcatag ggttcaaaaa tttcccggaa atcaacattg gcctgctcgg
     1561 cctcagggtt attcggaacc atgtccgttc cagctacagc caattgctgt acataatcac
     1621 ctccagtctg atagggcaat tcccacgcaa tttgaggtaa ttccatttta gccaaccaac
     1681 gcttgccatc cgttcgaatt tcacgatgat tcacaaaatg aagtagatga ccaaccccat
     1741 cgatatccca accatagcga atcggtgtcc ctagcgtgac aacatcgacc gctgctccat
     1801 ttaacgccac ttgatccatc acaagaccat acatacgttc gagttgggct acggaccgat
     1861 ccgctgaagg gcacgactgc cagtatttgg ccaaaatctc gaatacgcgc gccctcgcct
     1921 ctgattcccc tttggtgaga atattcgaca ataaagccag gacctgccca gcatgaccat
     1981 gaccgacgag aagaacccga tcatcttttg ttaagtcaag actagagccc catttttgaa
     2041 gatacattaa gaggtgaatg gcggcttcga ctcggccaac atgatgattt accgaggacc
     2101 aaatgtagcg attacaagga atagatttct cgctcgccgg agtcagggcg agttcaatat
     2161 tccttacata cgacgaagaa aaattcccta tttcctgagc gagcaggtcc aaccttttgt
     2221 gcgttgcttc atcgtctgac acaggggggt ggagggaatc tcctgggaaa caaataccgt
     2281 ttgtggcagg gcgaagaaga gccagcaacg attctaaacc agatattccc cgtgaatacc
     2341 ctcgttttaa acctccaacc tcatctaacc tggcagctcc aaagagatcc ataaagggaa
     2401 gaccctggag aaagagtacc cccttcactc gggattgggc catgtgttcg ccaacgccag
     2461 ccatggcttg gccccactca ggagagtccg gagccgctcc aggctgaaag gttcgatagc
     2521 ccacccgtgc accagggtcc gactttgaat aattggcatg ttggaaatta ttttcgtgag
     2581 gcatcgcgaa ccatccatac tgtgaatacc cccattcata atggataccc cagaccgagg
     2641 gcaagagaag cttgaaaatt tttggggtcc tttattatgg aaagaaacgc ctgggcttct
     2701 cagttggttg accgcctgaa aatctgcatg ataagttcaa atccaaccac tcatctgaca
     2761 gtggaggaga agtttcctac tcagagaatg taaggttcat tcccaatttc atctatattt
     2821 tcttttcctg gagcagggca gaaattatga gcacttccca aatacctacc ccccaacatc
     2881 gtcgtggtaa acgcatgcgg tgttttttct tcgcatggag tttggtctgg cttccgctaa
     2941 tcggaggaac agcttcgatt gcttctgcca cacaagccga acacatcatt ctggttgtat
     3001 tggaagggat caaatcatca tccatccaaa gcggtgccac cccaaacctg gctcgtttag
     3061 ctaaggaggg agtggtgacc tgggccgctc actccattgc cccacctctc actgttccgg
     3121 cgatggcctc tctgctcacc ggacttcaag tcgagaagca tcgggtgacc gccgaatggg
     3181 aaacatacga ttttgcacgt tcatttatgc gttcacccac agtctttgat tacatggatc
     3241 tggctggcgg aaaagacaca ggcgtctttc ttatggatga acgcctctat caattgattc
     3301 gacctgaaat ttacgtcgat tcccaagtct gtggctacac caaaccccaa tgcacgcctg
     3361 aaagagcgtc ggcatacatc aaagactttt taaaaaaagt caccagtgag aaaggctatg
     3421 gcttccggct ttttggagta ccaaacctct tattagtcca cctgcctacg gcggtgaagg
     3481 tcggacaaaa atacgggtgg gattccgaaa aatactccga tgccctaaag gcaatcgata
     3541 ctactgtgga acaaatcatc caaacgtata aagaacttgg ggtattggac aaaaccatgg
     3601 tgattgttac tggcttgaat agcggacccc tttcctcccc agtaggaggc gtgaaaccag
     3661 tctctaccag tcctacttcc cctgagttag atgccaccgt cccatggatt gcctggggcg
     3721 cgaatattaa gaaaaatcac cccatcaccc aatcagtctc tttgttagat acgggagcaa
     3781 ccatcatgta tgccctcggg cttgaaacgt atacagaatg ggacagccac gctattggtg
     3841 aaattttcca aaccgttcca gaacgacgga ccaccggcaa tgaaccataa aacaaacaat
     3901 aaaaccatga ttcgaatact ttttccgcgt caattcacac atatcgtttt ctgcctgttg
     3961 ctcatgggaa tttctccact gcttattcag ctcgggcaca ccgcagactc taacaaccct
     4021 ttgttgcgac aatttccaat taccgtgaat ccgagcgccc tcgcccctcc gacatggtgg
     4081 cacgtacccg gcatgacgcc gttgatttgg acaaaagatc ccattagtag tgacgcctac
     4141 aaaacatccg agaccagaac actgaacctc aagcctggag aatatcgctt tgggactttt
     4201 acgtttgatt tcttattcaa ggtcacctta tccgggacct tggaattctc tccaacactt
     4261 gatcaatgtg tagacggacg aggaacacaa gtcctgacaa ttcgttgtag ccatacgcaa
     4321 ccctatcccc aagaaccgga ctacccgaaa taatcggaag ggataagaag gaaaaaaatg
     4381 tcgaattctg tgcaagactg gattgatgcc ctggaagatg ccgatgatgc cacccgagag
     4441 gaagcggcca aggcccttgc ggaaaagggt gacccgaaga cgctagaacc ccttctgaac
     4501 gccctcgaag atgactattg gtcggttcga tcccatgtcg gatgggcttt ggcaaagatc
     4561 ggcggagaaa aagccgttga aggtctcatt acacttttca atgacaatat gatggaagta
     4621 caagctgaag cagtaaatgc catggcctcc atgggaaaaa gcgtcattcc gcaattactg
     4681 acttgcttaa aagacaaacg gtggcgggtg cgcgaacaag ccgcgaaaac gttaggcgaa
     4741 ttgcgtgacc ctcaggccgt ccaaggcttg agcctggtgt gtcgtgaccg agatggtgca
     4801 gtcaagagtg ctgcagccga agcattagga aaaattggag accccaaagc cataccaacc
     4861 ctgattaaat tatttaaaga tacatccaag atcgtgcggg aaaccgccgg aaccgcattg
     4921 atgtacgttg gaaaagaatc agtcgatgcg cttctggaat ccctcaaaga cccccatttc
     4981 gtcgtgcgct gtcacgccgt tcgtgcgcta ggaggcatga ccacggatta ccaaatggga
     5041 cgggtgtggg tacgtgaacc acgtgtcgtc gatgccttga tcgatatggt caaggaccct
     5101 gaccgagccg ttcgcgaaga tgccacaatt gccttgggca atattggaga tgcacgtgcg
     5161 gtggatgccc tcatagaagc catgaaagat ggaacagtaa aacggcatgc aatcgcttcc
     5221 ctaggcatga tcggtgatcc tcgcgcctac cctcccgtcc tggacgcctt aaaaggtaaa
     5281 gggatacatc aggaggggaa gccaacccct ggctgtatta tcagcgaaga cctcttaatt
     5341 aaagaagcgg ctgccacagc ccttggccat ttccgacatc ccaaagtcat tccggattta
     5401 gtgctgttac tcaaagacct agtccttcgt gattatgccg ccaattcctt ggtcttgatt
     5461 ggagatgcgg ccattgagcc cctggtatgg ttcttccatg acccggattt atcgaaggtc
     5521 cagcaagaaa gcgaaagggt tttggccttt gctacttctc gaatgacagc aggaggtgct
     5581 ctcaagaaaa cgatcctaga taccctggat aaactcgggt ggaaaccatc agaaggcatc
     5641 agcaaagaga ccagagagat tgaatatacc gatacttcca atgtgttagg tgagggagga
     5701 gagggccgct ttggcaagag cggggacttt gccatgccgg ccaaaccacc taaaatcgaa
     5761 cggtaatata ctccaattcc cgtgttggaa tgtgacgggg ctccgctcct gacggatgcc
     5821 ccttcctcaa ttagtctcgc cggaaatgca aaattcccca gctgaggtgg ccacttttcc
     5881 cagcctctac ccaatgctgg agacccacct tcatattagc cagataatcc tccgtacaaa
     5941 cccgaataag catatgatgt tgggtggtaa gttcctgaag cactcgctcg taatgtgaag
     6001 caaggttcgc ggaaagatcc tcaactccaa cctctttgaa tccaattttg gcagccaact
     6061 tccgatagta ttcaatcgat cccagtgaat ccaaatgaat acgatctaaa ataggttgaa
     6121 gcacatcggg agaacaggtt gcactcttca tcggatcggt aaaaataaat tgtccgccct
     6181 tagccaacac ccggtggact tcctggaaaa ctttctcccg atctccgcta tgtaaaatgg
     6241 catcttgcga ccacaccaga tccacgctcc catccggcat gggaatcgac tcaaaattcc
     6301 cgtcaatgac attaatggct agactgatat tctccttctg attgagttct ctattccgct
     6361 tattttggac ttcactcaaa ttcaggcatc caacctgaca tccatatcgt ctcacaagat
     6421 gacgggcgga acctccgtac cctgacccaa tatccaatac ccgtgtcgtt gaatcaagac
     6481 ctttgacaga agccgccatt ttctcaacgg ttcgtcggct ggcatcgaaa atcgagtcgt
     6541 tgtcggatgc gtacaatcca atgtgaatat cttccccgcc ccagattgtg gcataaaacc
     6601 ggtcagcatc cgaactgttg taataggccc gagcagtatc cacggtggga gaataattca
     6661 gattgccaat cttgcccatg atcgtggggg ggcgttcctc atttttatag cgtttctcag
     6721 ctacatgaac ataaaaatcg gggtcctcaa ctttatgcgt ttcttgaaaa tccgcatagg
     6781 tttttacttg ttgaaagcca acctcattca tgagccgacg tgtatattct ttccgcagcg
     6841 gaaacatatt caaatgaaac gcttcgccat cgggaaaagt atatttgaat ctggcaagcc
     6901 cttcatcaac atgttccggc tccgcactca cattgtctcc gcaatagtaa tacacatgct
     6961 ttgaggtaaa cccattatct aaaattccat cataatttct ttggtccaga atcaataccc
     7021 catcgtgctt cagcgcggtg taaaattcgg ccaaggcttt tcgccgatcc tgctcggaaa
     7081 agagatgagt cagagagttc cccaggcaaa tcaccgcatc gtatttattg tgtatatccc
     7141 ggctcaacca gcgccaatca gcatgaatcg ttcgcataat aaacccagct cgtctggcat
     7201 tttcaaatgc ccgtgctaac atttcaggac tgccgtccgc actcgtcact tcgaatccgg
     7261 cacgcatgag ccgaatagaa tgaaatcccg ttcccgtcgc gacatccaat acgcgtttga
     7321 cgccccgttc cttcaaaatt ctgataaaaa agtccccttc actcgaggcc cgggcatccc
     7381 aatcaatcaa ggcgtcccat ttttgcacaa aactatgaac atactcctgt ttgtacaaat
     7441 ccgttttacg ttgggcaatg ggattatccc catatttttg cttactaact ggcatcgtcg
     7501 ctttctgagt cattagggga aacctcctgg atggctacag ccctcaccac tgcttgagaa
     7561 tgatgggtac aaggttaagt aaaggcttgc gatttacaca tttcattaca gtattcaagt
     7621 gaaaaaaccc gcaagggcaa aacaatagtc tgtctccacg gaccagaaat aaaattgggc
     7681 tccgcatttt tcctacgcgg gtaagaaagg ccccactgtt gtgtgtgaga ttctcacact
     7741 aactcgttag gcccatcttc gcaaccccct tttgatgacg ctttcattcc ttcatcaact
     7801 cccaagtaca aaattcagtc ggtagcctta aaatcttagg acgaaaaaaa agctgattta
     7861 ccgccaccta aacaaaattt gcctactcct tgccattctt ttcggtttgg caataaacaa
     7921 aggtttcctt gttcaacaat gccaagtatg gtaaggccta accttcccat tcatttctat
     7981 cgccccatga acccactgaa atctaccacc cttgttttca atatctcctg cgccctcgcc
     8041 tttctctttt tgctctgggg tgcctttgcc ccgaaacatt tagcaaaaat caccgggacc
     8101 gtccaaacgg cccttcttga ttcgtttggc tggttatatg tcctggcagc aaccggtttt
     8161 cttatttgcg cgttctgtct gattttttcc cgatggggtg atattccact tgggcccgat
     8221 ggggccaaac ctgaatttcc tctcctcacc tggtttgcca tgctgttttg cgcaggcatg
     8281 ggcatcggtc tggtgttttg gggcgtggcg gaacccatat cccatttcca tgatcccccc
     8341 atcgacgaag gaggaacacc ccaagccgca cgtctggcgc tgcaatactc ttttttccac
     8401 tggggcctac acccctgggg catctacacc atggtgggca tggccctcgc ctactttcag
     8461 tttcgaaaag gcactccagg gctatttagt gcaggatgcc agccggtttt gggcagacat
     8521 gccaccgggc cgatgggaac ggcggtcgat gtggtcgctg tttttgccac ggtctttggc
     8581 gtagcaacct cccttggatt tggagccgta cagatcagcg gaggattatc ctatgtgttt
     8641 gccatgccca ataccctcac cactcaattg gccgtcatcg ccactgtcac cgtgctcttc
     8701 atgctctccg ctcaaaccgg actccaacga ggcatcaaat accttagtaa tctgaatatg
     8761 gtactggcgc taactctctt gtcgttcctg ctgtttctcg gcccaacgca cttcatcatg
     8821 accgtgtttc cctcgacttt cggaaactac atccaaaacc tcccgacgat gagcctgaac
     8881 ctcgcaccat tccgagattc aacctggatt cacaactgga ccttgtttta ttgggcatgg
     8941 tggatcgcct gggcgccctt tgtgggaacc tttattgcca gaatttccaa gggccggacg
     9001 gtgcgacaat ttgtgttggg cgtattgctt gttccatcaa tcttttgtgc attttggttt
     9061 acggtgtttg gaggaaccgg gatagcatta gaactctttc aggacgtgtc cttggaaacc
     9121 gtcatggaaa agcagggaat cgaggttttg ctctttacgg tgttggagca atatccaatg
     9181 ggcacggtca tgtcgctcat cgccattttc ttgatcggca cctttttcat cacctcagcg
     9241 gactccgcta cttttgtctt gggaacccta acgacgaatg gcagcctcaa tccccccaat
     9301 cggatcaaat tcacttgggg catcattcaa tccctatcgg cagccatcct actttggtca
     9361 ggcggactca aaggcctgca aaccggggcc atcctggctg cctttccgtt tgtcttcgtc
     9421 atcatcatcc tcatcatctc tctcctcaaa tcctttcagg aggaaaccag gtaggcctgg
     9481 tgcgacaaag gacacaatct ctcaatagtt gatttgatca aaaaaaatcg ctattactgc
     9541 tagttctttc aaccctacaa aaatttacct gtcacagaaa tattagagag gaatcaatga
     9601 agttagactc acttccaaca gaatttacgc cttacaagca gttagagatt gcgacgaata
     9661 aactggtcga tggtgtggca ttagtatcgg tcaatggatt tattccggtt ttaattggaa
     9721 gaggagaaac tcctgaaata tgggtgacca ttcctgccga tcaacagggg aaggaatggc
     9781 gtccaatcgt gagtaaaaat tcatctttgc ttgatgtgat aaccgtcact gccttgggaa
     9841 agaagatcga agtcgccttt gccgacgaga ttgttctgga agtcaaagaa attagcgggg
     9901 aaaacgccga aattacgaaa tgcaaccttc ggccatttgg cattaatgtg tttttagagg
     9961 acgactgtct gaaagtcatg acgaaccgct attcaggcaa caccataacc gaggcagaaa
    10021 tattcattga tgtagggaaa ggataaccac ggcaagatca gtcgaaatgc gctcgaccaa
    10081 ccgcatattt cttgatggta aaccatcatc gtgaatccca acttattgcg ggggcttcac
    10141 cctattaaag gacgcacacc cagaaaactc caaagggagt caggaacgcc attttatcgt
    10201 gcccgtctga ggccaaccag gttgcctttc cactagtctt tgtcatcatc accctcacct
    10261 atctcctttc tcaaatccct caactgtcgt ggcaaaagac ggcaacacag aaaatcacgt
    10321 gtgacacgga agtcggggac acaaatgaaa tgcattctcg atccagcgct ttttacagga
    10381 aattctcgag atggatcccg atccacaccg taaatctgga aggccagaaa tttgaactct
    10441 gaatgatcca agcacttctc attccggact tgcgccattt catctttatt gtcagaatgg
    10501 cgatagaagg cctccctgaa ggaggtcaat gattcctact tgactcgagt aaacggcaga
    10561 acccgaaagg aacgatcatg actgaccttg aaacgcccct cccacatatc accaccaccg
    10621 aaaccttagt gagattttat gtccttctct cccaatacat cgatcgatgc ctggatgagg
    10681 ccaccaagcg ctcactcccg gaaggagaat ttcaaaaaca cctggccgag actcacaccc
    10741 aggtgacgga acttctcgcg acaaaccggg ttgtcaaaaa taaggcggag acagaatttc
    10801 aacggatcac cacgctgtgt gaacaatttt tgaaatcccc aaccgatcag gtcattaaag
    10861 acaacctgaa ccacgagcat gacgttctga gaataaaaat gttagcgttg agcgacctct
    10921 tagcggtgtt tcggtcggta tagggtaggt attcaaccgc cttcacaggc aacctctctt
    10981 ctccctcatt gacccctcaa aaaatcgtgt ggtaacgtcg accttcgggt tttccctaat
    11041 ggtagggaaa ggggtgtggt ctatattcca ccacttggca caaacatggt cgactccgtc
    11101 gctgaacata tatctgcgct tgacgacgag gattgggggg tgcgtgaaga cgcagcggta
    11161 gccctcggac gtcttggcga tccccgtagc gttcaaccat tgattcgggc gcttcgtgac
    11221 tcagatcggg ccgtacggga agctgcaacc tcagcattaa aagcgcttgg ggaaccggcc
    11281 attctctcgt tagggttttg ccttcaggat gcaaatcttc aagttcagga atcggctgca
    11341 tgcatcctgg ctgatatcgc taacgaacaa gtcttagatc ccttgttgtc cgcggcatta
    11401 agtcctaact ggattgtgcg aatgtcggca gccaaaggcc tcagtcgcat ccagaacccc
    11461 caagccatag atacgttgat tttgctccta caggataaag tgccagccgt gcgagaggaa
    11521 gcagggcggg ccattcaagc catcggcaac accagcattc caaaattatt ggaaaaatta
    11581 aaggatgaaa actggaaaat ccggttgcgt gcagtggaag cattggccct gttgaagccc
    11641 ctggaagcgg taggacattt aatgattctg gtcctcgaag attccgatac tgccgtgcgc
    11701 caggacgccg tgcgagcact tggccagatt ggcgatcccc gagccattcc tcttttactc
    11761 tcgtcccttg ccctcgacac gccttcgctg aaattgccct cgattgaagc acttggccaa
    11821 atcggatcca ccgaagccat tcccatgtta atcgcgttgg tcaattcgct acccaaggaa
    11881 acctacgaag atcgcatgga aggatgcacc gacccgcaat ataaagaaga tcttcctccc
    11941 ctggaagctg ccatcagggc cttgggaaag atacgcgacc cacaggctat tcccgctctt
    12001 atacaagccc tgcaaagtac gctcctgcgg acagaagccg ccgaagccct gacgcaattt
    12061 ggccaaaaag ccgtgaatcc gttgttaaac ctcttaaaaa ccactaaaaa tgacaatctc
    12121 cgtcgtcatg tgttggaatc cctttctcac ctcggctgga ggccgggaca gatacgcatc
    12181 taaccgaaag acctgacgat gccgaaagaa actttcgaaa ccatcatctc tgaactggat
    12241 cacgaggaag attggcgccg aatgcgggcc acctccacct gcatgaaggg gggacccaag
    12301 gccgtcgaag ccattattca agccatggcg accggttcta cccactacaa ggtggaagcc
    12361 gcaaaaatgt tggcccgcct acgagatccc cgagccggcc aggccctcgc tcatgtcctg
    12421 aaggatgaag atgaacaggt gagacagtct gccgtggatg ccttggaaca tatggctggc
    12481 attttggatg aagccaccgc ggctgcactc ttagaacatt tacgcgacga aactctccag
    12541 gaaaaagtca cggaactcct cggtgtgatt cccacctcta tcggtcctct gtcggaacgg
    12601 ctcaaggatc ccgacccagc catccgcctc cgggccgcag agattctcgc acacctcctt
    12661 gatcctcgat cggcaaatgc atttgttgat gccatgtccg accccgatct tcgtgacgtg
    12721 gccaccgata ccttgcgcaa attaggtgcg attcgagacc gtgtcgatca aacaatggat
    12781 gatctgcgag cggtggacga atccgagtta aaagaaggcg tgcggcagga agcggtcatt
    12841 caactgcatc gtattggccg cccaatcgtt gaaattctca ttgaatacct ggaggacgat
    12901 gattggatcg ttcgggaagc tgccgctgat acccttggca aaattggcga tattcgggcg
    12961 gtggaaccac tcatggtcag gctccggtcg gacagcgata cgggtgtaaa agagcatgct
    13021 cttaaatccc ttggactcat cggcgatccc cggccggttg agttattcat tgaatccatt
    13081 cccattcgcc cccttcgtat actcgcggtc gaagccctgg agaaagtgaa agaaatcgaa
    13141 gtcctacgac cccatatgga attatttgcc agattaaaaa cggaccggga tggcctgatt
    13201 tcctataatt caggggtcat ccttgacaaa ctcgaggctg ccactgcaca aatgagtatg
    13261 gatgaagaag cctgggccga aaaggaataa aaggagtgtt gacccatgac ggaaggtaac
    13321 cacgtagatc ccttattgtc agcgcttcgt gatgacaatg aagcccttcg gaatcatgcc
    13381 gcctctcggc tcggagaagc tggctctgaa gcgattccaa agctgatcaa tatgttcttg
    13441 gaagacgatt gtgtggttcg tgaagcagcc acaagcgcct tggttcaaat tggagaatcc
    13501 gccgttgatc ccctgattga ggcgttgaaa gatgatgagg aatggtctgt tcgggaacag
    13561 gcagctaccg ctctcgggaa actccgtgcg attaaatcgg tggaaccatt ggtccaggcc
    13621 ctccggcacg acaaggatgg aggtgtgcgc actgctgcca tttgggcgtt agaacggatt
    13681 gggcatcctg atgcggttcc cgccttagtt gatgccttga tggacgcgac cctccgtgag
    13741 gatgccgcaa gagtcttaaa gaaattgggg gattcgcgcg cgactgatgc cctggtggaa
    13801 gggttacagg cctcgaattg gattgtgcgc cgacacgcgg ccgaggcgtt ggggaaaatt
    13861 ggcgatcccc gcagtttgga tcctttaatt catgctctgg ctgatgagga ctggttggtt
    13921 cgccgcaatg ccgccgaatc cctagctcgt tttaaggata gtcgagccat cgatcccctg
    13981 gtccctttac tcgaagatga aaatgaaatg gttcgggaca ccgccgaagg tgccctggca
    14041 agcttgggat ggacacccgg ttcttcccga tcataacaac aaaagctctt ttcgtttttt
    14101 taactacaaa taaggacact gaacaatcat ggcaaatgaa gcacccgcaa aactcatttc
    14161 aatcggcccc aagagtggca cgaaaaaaga cgggtttaat cttgtgactg aaaacgtcac
    14221 tgcgatcaat cttgatgaaa gacaaatcga ggtcgaactc ttggcgtacg atgggaagac
    14281 cgtcctaatg gatgtcagtg aagaggcggt cgaagactta caaaagatca aaatcggtga
    14341 cggggcgact cttcgcgtcg ttgaagaaga cggcaaacga atcgtcaaaa gttttcgcat
    14401 ccgctccaag gaccccaata ttcaacgagc cgatgctgca ctgcaagatc ttaccgattc
    14461 tcactggctc aatcgcaagc atgccattga agtgcttggt gaactccggg tggaagcagc
    14521 cgtgaaaccg ttagtagaaa tgctcagcga tgaggtgggt gatgtcagac agcgtgccta
    14581 tgaggccttg atcaaaatag gcgcgccctg tgttcccgat gtcattccct ttttggtgtc
    14641 cgaagaagac gatcttcggc aatcggcaac cgaaattctt cgaaagattg gaaagcccgc
    14701 agtcgagcct ttagctctgg cattaggcga ggctgatgaa aaacttcaaa aacgcatcat
    14761 gaaggtgttg gaccgcatgg gctataaacg gaaataggca atcggcagga tacacccaaa
    14821 gccatcccat cactagttct acaagacccg gaaggcaacc gtaaacgtct tgtgccctaa
    14881 tcctggcttt tctcgaaaca ggcttcccaa ctaagctagt cttcaagcac gaaacgtgtg
    14941 ttgcgatctt ttagcttttt tgggaaggag gggctcccac ggtttttcgt cccaccgaaa
    15001 cataatgaaa ccccagatct tccatccttt ggggttcata aacattgcga agatcgataa
    15061 aaatcggaga ggttactgcg gcccgaaccc ggtcaaaatc caaacttcta aactggttcc
    15121 actccgtaac caacaccacg acatccgccc cttccatcgt gtggtaggca tcctgacagg
    15181 ggatcatggt gggcaacacc ttcatcgata tctccaacgc ctccggatca taggtccgca
    15241 cggtcccacc cgctttcatg atctgctctg cgaggtacaa agccggtgaa tcccgaatat
    15301 cgtcggtgtt gggtttaaag gctaacccga gtaaagcaat ggttttcccc cgcaactccc
    15361 cgaccgcttc tcgaattttc tccatcatcc ggactcgctg ctggtcgttc acccgtttgg
    15421 tcgctttggc gatagtcatc tcataattat tttcatttcc gatattgatc aacgcggcgg
    15481 tatcttttcc aaaacacgat cctccatacc ctggacccgc atgcagaaat ttcgacccga
    15541 ttcgtttgtc taaacccatc cctttggcca ccagttgcac atcggcccca accccttcac
    15601 agagattggc catttcattg ataaaggaga ttttcgtggc caagaaaaca tttgaagcat
    15661 atttgatcat ttcagcggtt ggaatattag taatcacaat tggcgtttca aggatataca
    15721 aggggcggta caaatctttc ataatcgcca ccgattctgg actgtcagct ccaataacca
    15781 cacgattggg ccgcaaaaaa tcctcaatag ccgatccttc gcgaaggaat tcaggattcg
    15841 agaccacgcc aaaactacac gtcgttgttt ggttcgcctg aataatcgac cgaatgcgtt
    15901 ttgccgttcc gaccggcacg gtggatttgg tcaccaccac cttatatccc gtcatccgac
    15961 ttcccacatc tttcgccacc tgatcgataa aggacagatc ggctgaccca tcctcacggg
    16021 atggagtgcc tacggcaata aaaataacaa gggcttcatc aacggcttta tgtaaatcag
    16081 tggtgaatcg aaggcggccg gccgtcagat ttttggttac gagttcggaa agccctggct
    16141 catagaaggg caccttgccc ttttccaaag ccgcaatccg gctggcatcc gtgtccatac
    16201 acaacacatt caagccaaat tccgcaaaac aagcccctgt gaccaatcca acgtacccgg
    16261 ttcctatcac gctgacatac atgaatttat cctccttgat ctcaatatga tatttccatt
    16321 ttactcggtt aaagtcttat tgcacttaaa aaagaacgaa ttagtatacc ctattcgatg
    16381 ttgcttgaca gcctccaggg tgccaactag aatcagttca aaatttccta cagagcggga
    16441 atagctcagt ggtagagcat cgccttgcca aggcgagggt cgggggttca aatccccttt
    16501 cccgctccaa cttcaaccgt gtcctttccg aattacaact ccctctctcc ttcgccctat
    16561 ttctcgggct gccgaggctc aacttttata ttcattccgt ctaggggttc ccacagacct
    16621 tcctccttgg tcggccctgg aattttgacc aatatctcta agtcctcatt caaatctttg
    16681 agaaaatctt tcatttgccc gactgtcatg gatcgtgcct ttctataaaa attgttaact
    16741 actcttaccc tgctggcacc tcaattcttt cctatactac ttcaaagaat aggtgggccc
    16801 tgacgggtct cattaacgaa atttttccca gcttcccaca cccagggaag tgagtatttc
    16861 ttccgttcaa tcggtcacca ttatcaccca ccatggggtg gattcttctt tcatctcgga
    16921 aaactgcctc gcggtaaaac tgctggggac tcccccaagc accgctcccc gccgaatttc
    16981 cacataacga aacctggggg gcctctgagt accctcattc ttcacgaccc caaaaggcat
    17041 agaatctcgc aggtcagcta agacggaaag gtcttttctc tgtccgacag ccatggaggt
    17101 taattcctca tacacgcttc caaatattgc aggcagaact gtattcgtaa aaatctcatt
    17161 tcttggacgg aatcgatcat ccgctaacaa ttggctgagc acctcccaaa agaccccatc
    17221 atcgattgct gaaagaatac ccacacgact tcgttgagcc ataaatttca acaatgccaa
    17281 agggcccaaa atgtcaaact tccaatcggg gaaggttaat gtccgtcctc ggtgagagac
    17341 ttctaatatg ggctcccctt catatcggct tttccgataa tttccccggc tggattgaag
    17401 atgcgcatga ataatttcag gtgccaaacg actgggttca taggcaaagg tgggatttgc
    17461 cggagcatgg caggtactaa cggtgtaacg ggacaacttc cctttgctct tcgaaaatgt
    17521 ctccagaccc atgccccccc aaaatttaaa tcgtaattct tctgacgaga acccctgtaa
    17581 gtaacgctca cgctcaaggt ttttgtgata gacgccgccc acccgtgacc ccggcatcaa
    17641 ccgatcaaag gcatgcaagg tcagcggggt acgttgcgtc cgtaagtatg ggacatagtg
    17701 atccaccaat tcctgttcaa aggacaaatc tccagctccg atatctaaca gggagggtgt
    17761 tggcgtctgt cgtaacacgt cataaggatt agacacatcc cgaagaattt cctcccatcg
    17821 tatcccagaa tacacttccg gactttccct ttcttcattc acgttcaaga ttccccatac
    17881 catgagcgcg cgaaccgcct tggaaggggg aaagccggtg aggtccttga ggatctgatt
    17941 tccctcctta ctcccgatag gttgtgagaa tttttgcagg gcagcaatta atcgttcttg
    18001 aattgttgga tcgagagagg cctcttgaat atgttgaatg agggcgggaa gcttggcctg
    18061 aagggtttca ggaaccataa atttcctact cgcctcccag tgatgggggg aggattcccg
    18121 acttattttg attctctccc gaaacgccgt taatccgagt ctctggtcat ccattgatgt
    18181 gtatcccttt gactgatggc taacctaaat tcttcaccca ccttagcccc tccgaatatc
    18241 ctgaaacaac cgcatagctc ctaaaatatt gcccacaagg gagaactatg ctacaccttg
    18301 tttcgtcatg gtcacgcatt attttctacg tgcgaaacaa cacgttctct tcctctttct
    18361 gggcatcatt gtgatgagcg ccaccgccgg gtgtgcgaca tctcaagcca acccgacctt
    18421 tggagtcaac cacatctacg atgtcgacag gcagcaagca accacattcg acgatgtcct
    18481 cccacaactt ttagccgccg acgtgattta tatcggcgaa gaacattaca ccccttcaca
    18541 ccttgaggca gcccagaaaa tactgactgc cctccttgcc catgaccgcc atccagcgct
    18601 agccatggaa atgtttagtt gggatggaca atccgggcta gaccggtata gccaagaatc
    18661 cgaatatacg acagaacaat tggttaagga ttcagagtgg aacacgaatt gggggggaga
    18721 gtttgccgat tatcaaccat tagtgacctt tgccaaatca catcaactca aaatttttgg
    18781 ccttaacccg ccacgcccac ttgttcgatt agtcgccacg aagggactat ccgaagcctt
    18841 gcaagacccg gaaatgcgaa aatggcctat tcaagaaatc gtgaccgatg atcacgcgta
    18901 tgaacaattg atttttgaac aaattgaagc ctgccatcct ggcttaccag accacgtgta
    18961 ccgccgaatc ttcgaagcat ccatcttccg ggatgagggc atggcacaaa ttattaccaa
    19021 ttacctcaac acgcgatccc ggaccgccgg accgttggtg agctacacgg gcggcggtca
    19081 tattcagtat aagacccccg tccccaagcg agttaaacga aagcaccccg gtgtcaaatc
    19141 tattaccatt tatctcgaag catgggatcc atccaaagaa gagaatgttc gttcggagtt
    19201 gcaagccggc attgctgatt tcctctggct tacccctttg ggccctaaag gccttcaacc
    19261 ccgctgcggc taactaataa caccaatttt cttaacgaag gaaggaggtc taaccacatg
    19321 ggcgacccga ttatcattgc acaacgaaca ccttacgttc tgaacatgga agcaggaacc
    19381 tattattggt gtcgatgtgg ccgatcaaaa acccagccat tttgtgatgg gtcccacacg
    19441 ggaactgata tttcccccat cgaggtggaa ctcaaagaaa aacaaaaggt agcttggtgt
    19501 ggatgcaaat acagcaaaaa gcccccattc tgtgacggta ctcattccag actggaatcg
    19561 taaaatctgc tctcgactca ttaaaccagc tcgtcttttc aagatccttc gtgatcatga
    19621 ttacgtatca taaactaaca tgaatgcttt aattaaatta tactagttag aagcttgcat
    19681 ccatgaaata aaatttacta caatcctcca cgattgtgga tagttgattt ttatttcgaa
    19741 gctatcccca aacataaatt gtgtgagata tttttataat gtccgaaagt gaggattttc
    19801 gtatgaatcg gattacttcc cttctcagtg cttttctcct attagccgca aaccctgcat
    19861 ttgccaatga gccaggcgga ggctatgaag ggatcacggg cttgtactat ggaaccatag
    19921 cccttatttt ggcctatggg gtctatgaca ttttcttcaa aaagtcataa cgtctcccgc
    19981 attccctaac atgccattta gaggattccc cttcccatta aagggggagg ggtgcagact
    20041 actcttgggc ctgatgattc cgccgagttt tgagtaaagg ctccaaggtg cgaaaaacat
    20101 tctgagctac aatggtatag ccttccgccg tgggatggat tccatccgcc tgatttaagc
    20161 ccggttgggc cgccacacct tccaggaaaa atggaatgag tggaagcgtc aatttctggg
    20221 ctagccgttc atacattcca aaaaattctc cagtataggc ttcgccataa tttggcggaa
    20281 tgcgcatgcc cgccaaaatg accgctactc cttcctccag caaccggtca attatgctgt
    20341 gaaggttgag ataggtctgt tctaaagaga gcccccgaag tccatcattg acgcccaatt
    20401 ccaatatgac aaccgtcggc cgacttttta atatccactc tacccgtcgc actccccccg
    20461 ctgaggtttc tccgctgact ccagcattga ccacctcata atgaaatcca ccctcatgca
    20521 actgtttttc caactgagcc ggataggatt catctgatga cacgccaaac ccagccgtta
    20581 agctatcgcc aaaggccaca attttaggca gagattcgtt ctttggcacc aatagctctg
    20641 atgaggattg atcgggcggg aattgagcca gagaaatttc cgtgtctgaa ctgtggttca
    20701 cgggctctgg tataccggta gacggacttt cctgagggtc acacccccag attcccagac
    20761 agaggagcac aaggactgat aggatacatg tatgcataat ggccgggtaa taactctcat
    20821 ctctattatt caccatttgg acccatttgt gtaatcaacg tgaacgttcc tgcatcgtct
    20881 gtaccccctt tgcaccccct caccccatca tctcaggtcc tcatttctgt caatcacctt
    20941 gaaatggagc ttcaggcagc caatcagtcc gtcaagattt tacagaacat atcctttgag
    21001 gtcctcacta atgaggtagt cgctattgtc ggtccatctg gaagcgggaa atcaacactt
    21061 ctggggttac ttgcaggcct cgacaggcca acacaggggt ctatccgcat caaccagacc
    21121 gatatcacga ccttaaccga gaccgaaatg gcacactttc gccgcaaaca cattggctat
    21181 gtgtttcaag cctttcatct ccttcccaca ttgacggctc tggaaaatgt ggctctccca
    21241 ttggaattgc aaggcatatc cacgggcaca gtacgggcca aagcactttt acaatccgta
    21301 ggcctggaac accgtctcca ccattatccg gttcaactct cgggtggcga gcagcaacga
    21361 gtggccttgg cccgagcctt tattgtccgg cctcccctcc tgctggccga cgaacccacc
    21421 ggcaacctgg atagctcaac cggcgcaatg gtcattgatc ttctgtggga acttcatcag
    21481 caacacggca gtactctcat cctcgtcact cacgacatgt cattggccgc tcgggcacaa
    21541 cgaatcctgg ctctgcagga tggagcaatg gtgaacgaga cgagaccccc tttattatca
    21601 tccggctcag aggcatgacc ccagtatccg tcaaactcgc ctggcgggaa atccacagcg
    21661 cctggtcccg tttcctcttt ttatttctct gcattgcact cggtgtcggt gccattgtgg
    21721 ccgtagatct gtttgccgtc aatgtcgaac aggtcattct gggagatacc cgggccctgt
    21781 tagggggtga cgtcgaacta tcatggcggc gggcaatttc cgataagggc cgcaacgtac
    21841 tggactccct cgctgaccgt gacatgatcc tttcacacgt gactgaaatc gccgcaatgg
    21901 cgacagtgga gaaccctcac tcacaaacgg cttctcaatt ggtggaactc aaggccatag
    21961 attcggccta tcccctctat ggtcaattgg tagtggagcc ggatctcccg atgacgcaac
    22021 tcctggatcc ccttcaatcc ggttgcggtc gctcgccgtg ttttggtgca cttgtccagg
    22081 aatccttact gatccggctc aatcttaccg ttggcagcga gctacagatt ggcaaagcga
    22141 gctttctcat taccgccgtg ctgaaaaaag aaccggacag gattgccaat ggatttagtt
    22201 tgggtcccag ggtcatgatt tcccgagatg cgctcaaagc caccaccttg atccaaaccg
    22261 gaacccggat tcaggaacga tatcgcctgt ccctatccga ctctacctct cttgagcctt
    22321 taatgggtga gttacgcggg cgcctcagcc aggaaggagt tcgggtcagc tcatttcgcg
    22381 atgcccaacc acgactccgc cggttccttg accaattaaa cctctatctt gggctgattg
    22441 gttttacgat cttattggtc ggtgggattg gagtggcatg cacgattcaa ggattcctca
    22501 cacaaaaaat tccgattatt gccacactca aaactctggg agcggattct tcacaaatca
    22561 tccgtctcta cctcacacaa agtctcatcc tgggagggat cggcagtctt attggggtca
    22621 tagtcggcgt cattctccac cggggcttac ccctattgct tcaaggcatt attcctgaaa
    22681 ccatgccact gaatacctct ctcgcacccg tcctgcgtgg aatctttctt ggcaccctag
    22741 ccactcttgc cttctcactt tggccgttgc tggctatccg tcatgtctct ccggcattgg
    22801 tctatcgaca agcggtggat cactcccagg acatggccaa tagccaatcc agactaaccc
    22861 gatggcggac catcctgaag cgttggtgga atgaccgggc acaggtcatg gtcagcatca
    22921 gtatgatggt gggcgtgacc ggcctggcca tgtggcaggc ccactcactg accctcggtt
    22981 tatttttttc cggtgcttgt gcgatcgcgg ttctcctctt attagtcggc actggagcac
    23041 tgtatagaat tcttgggcat gtcccgattc ctcagtggta tttattgcct catgcggtga
    23101 agaatcttca acggcctgga aactttacca aagccatgac gttggccata gggattggtg
    23161 tcatgctcat gacaacgctg actattgtgc aacggtcctt acttgacctg ataggaaacc
    23221 agataccctc tcaggctccc tcattttttt tcatagacat ccaaccagac caatatcccc
    23281 aatttgtgga tgtgctacaa cagaaattcc ccgattcacc ctataaatta gttccagtcg
    23341 tgcggtctcg cctcacaacc atcaacggac aacccatcga ccctgaagag cataaaggcc
    23401 aacgaaatgg atggtatttc accagggaat acgcattgac cacatcccag gaccttccaa
    23461 aagataacgt cctcactcag ggacaatggt gggatcacac caaggaatca gactcggacg
    23521 gagcgcccgg gaggccttta gatatcccgt tggtctccgt ggaggaagac gccgcaaaaa
    23581 acctgggact gaccctaggc tcaaccctga ccctggatat tcaaggggtg ccaattgtca
    23641 cgaaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccc taggctcaac
    23761 cctgaccctg gatattcaag gggtgccaat tgtcacgaaa gtaggcagtc tgcggcaggt
    23821 cgattggggc agtttttcca tgaatttttt catgatcctt cagcctggct cctttgacgg
    23881 cgcacctttc acctacatcg ccacaaccag agtgccgact aatctcgaag tccccttaca
    23941 gcaagccatc gtcgccgtta tgcccaatgt gacagccatc aacgtgggag atgtgctgca
    24001 gaatattggt cgaatctttc accagttggc catgggaatt caagccttgg ccctgttatg
    24061 tctggtcacc ggagccgtgg tcatggttgc ggcgatttca atcaaccggt atcggcgcct
    24121 gcatgaattg gcaattgtga aagccctggg agcaagtcgc agactactcg tattttcgct
    24181 cggagtggaa ttcggtgtca ttggggcctt tgcagggctg gttggacttg gattggggtc
    24241 tctcctctcc tggtctctct tgtacctctt ttttgaccta acctggaact tcgatctcat
    24301 cgtgctaagc acaggtttgc ttttgacaat tctgctaagc ctcatgaccg gctttctcgg
    24361 gacgtaccgg ttgctgggct tccctcctct atccgtcctc cgtcaagaat aaaccccgtc
    24421 catcagattg ccgggttctt ctcccaacac aacatccgtg actgaggccg gcctctttct
    24481 tcgatgaaca ctcgcaggag ttatccattt agatacaaac tgtatgctgt tgaataacaa
    24541 cctgtcccac aactgatgcc cttatgcctc atcttctttg catcctttaa aaaattccca
    24601 aaccaatttt aaaaatttgc ttctccattc atcagattat caatgagtta taaccatgaa
    24661 agccttaaag agccgctccc ctactattta ggtacattct ttgctttgta atatggcaag
    24721 taggggatat taaggggagc tatggaaatt attttcgcca tctgtatcac aggctttgga
    24781 atcttcggag gaatgtccct catatggaat gggcagaatc ctataccatt aaaggcaccc
    24841 gacaggtcag gctcacggta aaatgatgac caaaacaaca caggcctgtg cctgcatgac
    24901 atttgttcag gagccatctt taggtgattt caccaaatgg tgagtcggcc atcaatcgac
    24961 tcgaatccac caagatggtg aattcgctca taggttagga aaggagatcc tcatggctaa
    25021 aaatattctg attattgatg atgatgaaat gagtcgagga ttgctcagga tggtcctgga
    25081 atatgacgga tgtgagtgtg tagaagccga aaatggagcc agcgggttaa cacttcttga
    25141 atcaaaatcc tttgacgtgg tgatcctgga taatgcgatg cccgtcatga ccggaatgga
//
LOCUS       DAORAQ010000030        24620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_382_length_24620_cov_64.309750, whole genome shotgun sequence.
ACCESSION   DAORAQ010000030 DAORAQ010000000
VERSION     DAORAQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 24620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24620
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_24620_cov_64.309750"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..422
                     /locus_tag="PKM72_04745"
     CDS             24..422
                     /locus_tag="PKM72_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNP60125.1"
                     /translation="MISTLSDSASFITVSVGNEIRVRAEQKSITGDTQRKSETKQVLL
                     LPEPAEDSMAHEPLTTEQVQSTVARLQETLKHIQPRLEISLERDINQVIFRVVDKESG
                     DLIRQIPSEKMVDLARLFSGQSGLLVEENI"
     gene            552..1967
                     /gene="fliD"
                     /locus_tag="PKM72_04750"
     CDS             552..1967
                     /gene="fliD"
                     /locus_tag="PKM72_04750"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNP60126.1"
                     /translation="MAISFGGLGNGVDFGPVVDALVQAERFPIDALTQKKLQAQKKQT
                     ELGLLGAKLLSFQGLGNSLRNRVSFNKNQVNVASASAQTPLSASVSSSAASGTYQVTV
                     NRLASAHQIISKASTAVSESDADIVSGASSTFQFQVGTGSIHTVNLDAASTLDDLRSA
                     INDLGAGVSASVLNTGSEASPQFRLVLSANETGLDQAITIVADDTNLDMVTTGVDTFQ
                     AAQDSEVVLGATDGEVGTTAITINRSSNTLTDVVTGLTLNLQAVDASNPVTISVSQDH
                     SAVKTAISDFVKGYNEIVTFVNERTFYNTETKERGIFVGESLARNVLDRVRESVFSQI
                     GGLTTYTGASQIGFETQTTDGTIKLNEATLDSALSTNFSAVRDLFVKNATTGTKGIAE
                     LVVDAIDGLDDIDTGSLTRRQNALTKQVKDFTSQITFKEEALTRFEQQQRLKFANLDG
                     LLARLQSQLKQMQNAFPTTNG"
     gene            1983..2375
                     /gene="fliS"
                     /locus_tag="PKM72_04755"
     CDS             1983..2375
                     /gene="fliS"
                     /locus_tag="PKM72_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNP60127.1"
                     /translation="MMVGAQSYANTQIQTASNVQVIVLLYDGAISSMKLAQEGMCALN
                     YPDKARFLDRALRVIGELSAALNMEEGGGIAKDLRRMYEYIQFELTQANLKNEPRRLE
                     GPIRCLSLIREAWVELAVQGVKPQAVGI"
     gene            2389..2703
                     /locus_tag="PKM72_04760"
     CDS             2389..2703
                     /locus_tag="PKM72_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60128.1"
                     /translation="MPEVNCLVEQLTKDAIEAAKNGQWDQVIVLYDQRLSQGPQQSLT
                     SEAVKFLVASDQWLVKRVREAQAAIQQHLNDMQDQRRKLEVLKRQWGANSTIQVRHLL
                     TI"
     gene            3021..3272
                     /locus_tag="PKM72_04765"
     CDS             3021..3272
                     /locus_tag="PKM72_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60129.1"
                     /translation="MTQQSASTANAHLSVLPDNTVQNGKLLHSLSNKLLPIVVFSELA
                     LRRCEDDQLKRQLEKIHGAAEQARDILVEIRSLYNGKSS"
     gene            3358..3963
                     /locus_tag="PKM72_04770"
     CDS             3358..3963
                     /locus_tag="PKM72_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNP60130.1"
                     /translation="MDIVASEDRRQDCRVDVPVCLWVEVPLAFSYETIDLDGAEFSQW
                     QWDELAVSPDLVKAMVDEKDLTIRDPLLLQMVTRIDWMLTSILRTLGKEKNLKGAIPE
                     FTKVSLCGSGIKFFSEDTYPLDSFLVLRLILRPFIPIQAVGKIVRVDSTRKGKEPGFE
                     IAVEFTKISSDDREAIIRHTLRSQATMQRLRLKQPVDSVLD"
     gene            3869..5641
                     /locus_tag="PKM72_04775"
     CDS             3869..5641
                     /locus_tag="PKM72_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNP60131.1"
                     /translation="MIAKRLFDTHFGLKPPCNGFGSSNLSIPFLIKASGIFCCLLSCL
                     VIVAGCQRAPDVPLSPHSVPSSSISSPPPRLLGRPYRVAILPFENMTRHPQLDWLRRG
                     LQEMLVSDLAQWSELEVVSRDVLGSVLREQWLQQRGFSSDENTVGLGRLKGVRYLIQG
                     RMYAQEEALAVDLQIVDVETGVVVGAVNAKGLESDIPGLEQDIVTRVLGMFDPSLNVE
                     TGSMSDQMAESSQLSSNRHLEIDRERGSLPFLGSVPSHAVNQIDALLSLEMLTHKRRE
                     AYWLAETIWHEGWVSEIGNPLHQVWQFPNASLSNLPIMAIPISLFFSPHRLAHIFEQT
                     QKTEPGYGGYVDSDGFRTTIDEASGAQLLFVEHFQKRRRVFIRAINEQGDVVAIFSDW
                     GWRTERKFTMQGTQGVSVPMWPNPFITGFVKFPVDWVEREGRHVTFDSVVVPVPDEQV
                     LVLLEPIGELQKEEIEISSQNDQEDIFLKELGNQIKSNWTPSITEGLPVRGYLPGNKR
                     TAVGIVHAQDGKIRDIQFQHWPEDPFFLKSLQDLQLKLLGACLECRNSGQVLPPLPAS
                     FKTFRLQLTLVKDIHALHLGSRSH"
     gene            complement(5693..7108)
                     /locus_tag="PKM72_04780"
     CDS             complement(5693..7108)
                     /locus_tag="PKM72_04780"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNP60132.1"
                     /translation="MAGSQPFSSPFDGVSLLADPIHGYISITVPYSVPQFTEQTEKDL
                     IDSPWVQRLRYILQLQSAHWVYPGAEHTRFQHSLGTMHLAGRFMVRLYPSLCQTVSEV
                     PSCHFLEEFIRITALLHDIGHGPFCHFFDHHYLHQFDLTHEVLGQHIIRDHLAPIIKK
                     ICRSPSGPFAPGEHLDPDHIAFLILKDPHKSTKGLPPWVIALQPLLGGIYTPDNCDYV
                     LRDSYMCGVAIGPVDIERLLHYTFFSSHGLTIHQSGLPALQMFLHARLYLYANVYYHR
                     TTRAIDIHLQEIFQPTMEILCKKNPLQSLPQYLHLTDWHVIETVRSWKHARSMHKRQL
                     GLEWSQIIGRHVKWKTAFSTLLPIIQPAASSFQPQVFLKRMRRLLPSKYRSQDIRVDF
                     AHKDPRPINLLNMGNFQIFVWNPAKGTVEKDSLQKFLQYLPARMITLRVFSQDHSIDK
                     ILANAAEETLRRHWPTLIQPS"
     gene            complement(7233..7682)
                     /locus_tag="PKM72_04785"
     CDS             complement(7233..7682)
                     /locus_tag="PKM72_04785"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNP60133.1"
                     /translation="MLTLESVRQQLTQVLHLQETPHRTALAFSLGVFIAFAPHYFFHT
                     ASVVFCAWAFRLNILALFLGSLVNNPWTLVPILAASLYTGMLLVGTSSSTTIEWDQMN
                     VDNMFDMLSPYLIPFFVGACALSIVGSLMAYPIMRWVMTRYRGFKNS"
     gene            complement(7704..8747)
                     /gene="thiL"
                     /locus_tag="PKM72_04790"
     CDS             complement(7704..8747)
                     /gene="thiL"
                     /locus_tag="PKM72_04790"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNP60134.1"
                     /translation="MSPSRRQAPVSEIGEFQLIRALNRGFSPPGSRTLIGMGDDAALL
                     AHPSSTHIVISTDLLVEGIHFSKNTASFYDIGYKAAVANLSDIAAMGATPTYILVAIA
                     LPSYVRFGDWKDLYRGLSVPCKSHGVQLVGGDTSASHSSLFLSITILGQVEPGHALTR
                     GGAKEGDIIYVSGSLGNSAAGLACLTHQGSRGKISNLRQPMKFLVRRHLQPTPRIALG
                     RLLSSRRLASAALDLSDGLSGDITHLCQQSRVGALILEANLPMSPHLLAYASQVGADP
                     LPWVLHGGEEYELLFTVSPKKQHSLESAIKRLRISATAIGVMTSRRSGIKITKKNGRT
                     HQLVPKSYVHFSE"
     gene            complement(8756..11233)
                     /gene="lon"
                     /locus_tag="PKM72_04795"
     CDS             complement(8756..11233)
                     /gene="lon"
                     /locus_tag="PKM72_04795"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNP60135.1"
                     /translation="MTEDYPEIPTPSIDQIPDELPLLPVRDIVVFPYMVLPLFVGRNI
                     SIKAIEAALLSPNRLIFLTTQKNQEVEVPETQDLYQVGTVGVIMRMLKLPDARIKILI
                     QGLTKARIQEFTQTNPFFSARIETCTELSTALPSLEKEATVRSTREALGKVIGLGKIF
                     MPDVLTVIENMEEPGRLADIISSNLGLNVETTQGVLEIEDPLLRLRRVNEILGNEQDV
                     LSMQQKIQAEAKGEMDKTQREYFLREQLKAIQKELGELDDRSEEVAEFRRKIQEATMP
                     DKVLKEAEKQLKRLEKMHQDTAEAGTIRTYLEWMVELPWNHHSEDNLNIPQASQVLHE
                     DHYDLERVKERILEYLAVRKLKANMKGPILCFVGPPGVGKTSLGKSIARAMGREFVRM
                     SLGGTRDEAEIRGHRRTYVGALPGRIVQGIKQAGTNNPVFMLDEVDKIGTDFRGDPSA
                     ALLEVLDPEQNHAFMDHYLGVPVDLSKVMFITTANLTDPILSALRDRMEIIEIPGYSE
                     EEKLGIAQRYLIPRQLEEHGITEEHLLLTDPCLARIISQYTREAGVRNLERELANVMR
                     KVAKHIAEGKIKQHQVTPRNLNHYLGVPKYVPDQEQEKDEVGISTGLAWTETGGDVLY
                     IETSAIKGKGLLTLTGHLGEVMKESAHAALTYIRSQAKSLGIDPDIFAKQDIHVHVPA
                     GAIPKDGPSAGITIATALASCLTNQPVKRDLAMTGEVTLRGRILAIGGLKEKVLAAKR
                     AHIKTIVMPKRNQKDLNEIPKHLLRGLQFIYVENMSEVLKVALRPKNRHTLNSAITHG
                     NNSSGRRKKVASSVTRRPLRQTVPKTR"
     gene            11931..14132
                     /gene="bamA"
                     /locus_tag="PKM72_04800"
     CDS             11931..14132
                     /gene="bamA"
                     /locus_tag="PKM72_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNP60136.1"
                     /translation="MLAQSSQSQIEAIKVEGNQHIEEQAILGKLSIKPGDPFTQESIR
                     EQIQLIYGMGFFEEVEVSTEVRAEGVLVVFGVKEKPFTIEVVFDGNEELSDDKLNEKN
                     VIKNQVFLDKEQVKISVENFRQLYHDKGFYHAQIIPITDIVENNQVRLTYYIEEGTQA
                     HIRTIVFEGRNVLQKKELTSFMANREYSWPWSIFSDAGILHRDELPNDVERIKEVYLN
                     KGYLDVQVGMPRMDLDETKEWFTLVFPIVEGEPYVVSQIQYTGNTLFSDAELREGLII
                     EPGEVFQRAKVRDEITRLTDLYGSRGYAFAEANPSVEPDPPTRSTRIGFSIEEGEMVR
                     IRDIRITGNNKTRDNVIRRELRVDERDMIDSPGIKRSFERLNNLNFFETVEILPKQIA
                     PGEVDLDVKVKEKPTGSFSIGGGFSTLDQFTLIADITEGNLFGRGYLARIRGQVGGRR
                     TIGTITFRNPAIFDTLTSGQADLFSSRTNYLTYLEKKQGANLTFGRGFSEYLTGTFTL
                     VAEKIKIDDVDSDANDLIKDQEGSQSTTGFRASLFRDSRDYYLDPRRGTRIGVRASFG
                     TDVLGGSNNFYSMAFDAMKFTPLPFWDLRHSIRGRIGYGDGFNGSDFPIPEFFYVGGI
                     NTVRGFKYGRAGPVTDGNDPEGGNKQIIFNNDLIFPVLPDAKLNGVVFFDYGRGFSQG
                     DKLDLDLRMAAGMEVRWISPFGPLRAAYGRPIDGPKDDRKWVFEFSVGSVF"
     gene            14184..14759
                     /locus_tag="PKM72_04805"
     CDS             14184..14759
                     /locus_tag="PKM72_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNP60137.1"
                     /translation="MRSSTDVGGMGKWMAFGSLVLSLAWGCAGMGSNKTSDLPVGVVD
                     PQRILAETVKGKRLSDALNAFMKDRQTLVELEQRELRKLESELMAQSTVLSQEARDRK
                     EEQFRAKMAGYQQKVADLNREVQEKQQELQNEFRLQVQRVVTEVAGQRGLGLVLEYGV
                     NSGTLFYEAGLDISTDVIQALDRESGAISVP"
     gene            14828..15283
                     /gene="fabZ"
                     /locus_tag="PKM72_04810"
     CDS             14828..15283
                     /gene="fabZ"
                     /locus_tag="PKM72_04810"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNP60138.1"
                     /translation="MVEGELDILHIQDLLPHRYPFLLVDRILEIQDGTRIVGIKNITA
                     NEQFFQGHFPGRPIMPGVLLLEVMAQVGGVLARKTAKEIGRPTVFLTGIEKAKFRRPV
                     IPGDQLRVEVEVVRRKDPFWKMAGKILVESSLVCEAEMTAMVKVEESEA"
     gene            15352..16161
                     /gene="lpxA"
                     /locus_tag="PKM72_04815"
     CDS             15352..16161
                     /gene="lpxA"
                     /locus_tag="PKM72_04815"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNP60139.1"
                     /translation="MNIHPTAIVHPKATVGEDVVIGPYCVVGEHVTIGDQTELVAHVC
                     VEGHTEIGRCCRFYPFVSIGTPPQHLQYKDEPTRVCIGDHNIVRENVTINRGTAFGSG
                     VTRIGHHNFFMAYTHVAHDCELGNHIVMANAANLAGHVSVGNSVVIGGLVGVHQYVRI
                     GEYAMVGGCSALGRDVPPFVRAAGGYRAQMFGLNTIALRRHGFSGARMNTLKAAFELL
                     FRQGHRLQEAIKLARNEYGESTDVMTFLTFLESTSRGICQVSSRELDDDEE"
     gene            16189..17058
                     /gene="lpxI"
                     /locus_tag="PKM72_04820"
     CDS             16189..17058
                     /gene="lpxI"
                     /locus_tag="PKM72_04820"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNP60140.1"
                     /translation="MISPEQDERIGLIAGNGRFPIIFADNVRRLGFSVSAIAHVGETL
                     PELESHVDRIHWLKIGQFSKALAALKGDGIRQAVMLGGIHKTHVFTALRPDLRTLAIF
                     SRLKHWKDDAILRAVAGELEREGIQIRDSTFGLEGILAGEGNLSYRKPTSKEWDDIQF
                     GWDTLETLGKLDIGQCVVVKNRVIVAVEAVEGTDGTITRGGTLGGKGTVVVKRTKPHQ
                     DLRFDLPAVGPQTIQSMVAVKASVLAIEAGRTVVLDRDEMFVNAKSAGIAIVGIAPMD
                     PLHSPAGSSDAPP"
     gene            17146..18102
                     /locus_tag="PKM72_04825"
     CDS             17146..18102
                     /locus_tag="PKM72_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNP60141.1"
                     /translation="MTQSIRVGVIGVGHLGRHHARIYGILPSVTLVGVSDTDTLRGHM
                     VADECGVRYFQDVDELLSLVDAVSVAVPTSVHGVVVTNCLQRGCHVLVEKPIASHVWE
                     GEQLVELAKVRGRILQVGHIERFNPIVEVMRPLVSQPGFIECHRLSPFQPRGTDVDVV
                     RDLMIHDLDLLLSLGLGPIMQVEARGMPVFTDLPDIANVRILFESGCLANLTASRIST
                     GRLRKIRVYQRDRYLSVDFGGKEAVMNTRRSLEGGTFEVETQHIKGDEGDALTRELDC
                     FIQSILGNPSARGVSGEEGVEALRVATQVVSLIQQHAGHSPR"
     gene            18151..19281
                     /gene="lpxB"
                     /locus_tag="PKM72_04830"
     CDS             18151..19281
                     /gene="lpxB"
                     /locus_tag="PKM72_04830"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNP60142.1"
                     /translation="MPKIFLVTGETSGDIHGAHLALALRELNPAVELIGVGGSRMLEA
                     GVSLLPGVKRVDAMGVPGIRQLLQGWKTLRTLACYLKHERFDVIILIDSPGLNLRLAK
                     AIAKRSHKIIYYIAPQVWAWGSRRLKVIRKVISHVLAILPFEEAYFQKAGIPCTYVGH
                     PLLDELKPAYDIVHERQQLGLEREDLVIGLLPGSRVREVREVLPVFLRSVEQIRSQYP
                     RIQILLAQAHSLSDSVVNEILGHVGQQVKVVKGRSNEVMAASNLLLVTSGTATLQSAL
                     IGTPMVVVYRASPLTYHIGKCLVKIPYISLVNILAGQEIVPELIQDRMTPDRIAHEAL
                     KILQDARRQNDMTQAFQAIRNSLGEAGASKRAAAFILAEAIV"
     gene            19278..21032
                     /gene="msbA"
                     /locus_tag="PKM72_04835"
     CDS             19278..21032
                     /gene="msbA"
                     /locus_tag="PKM72_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNP60143.1"
                     /translation="MKTLWRILSYLKEYRLRLIGAFVCSAGVAGLSAVYAWLVQPVLD
                     GIFIERNQEMLVILPLAVLGVAILKASFAYGQGYLMSYVGHWLVSDIRQQLFIHIVRL
                     PIRFHDANASGRLMARVISDVNEMANAIPSVLKDIFQQGLTFVAMLSVVFYQNWKLAT
                     IVLVVLPFSSLVLVRVGRRIRKLSKRGQESIGKMASVLKEAFSGIKIVKAYGQEEKEA
                     QRFSLTNQAFRTAKVKSSQTSAMASPLLEVIGVCGVAFIIWYGGGLVIAGEMKPGEFF
                     SFLAAMFMAYAPLRKMSGANESVQRALAGAQRVFHVLDLESEIAKDEGKKSLPPIAKD
                     LEFSAVNFSYEGSEEPALHEINFTIKVGEVVAFVGASGSGKTTLVSLVPRFYRPTEGT
                     VKIDGQDIRLVDRSSLRRQIGIVSQETVLFDDTIRNNIAYGCLDASEESVIDAARAAF
                     AWEFIERLPQGLDTLVGENGLRLSGGQRQRLAIARAILRDPPILILDEATSALDSESE
                     KLVQKALVNLVKARTTLIIAHRLSTVQHADRIVVMNRGRIEEIGTHSALLQQGGLYTR
                     LYQTQFLFTQPEPLPTIS"
     gene            21052..21567
                     /locus_tag="PKM72_04840"
     CDS             21052..21567
                     /locus_tag="PKM72_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNP60144.1"
                     /translation="MLNWMKLSVAPPLAARIIRLLGLTMRISTVGGEAVDELYRQGRH
                     IIIAFWHGRQLMMPLAYRGQQASILISQHRDGEIIARIMGYFGFLSIRGSSTRGGLRA
                     TRQLLKAGRNGGDLVVTPDGPKGPACTVQPGVIYLAKATGLPIIPLTFACSKKKSLRV
                     GIASRFPIPVG"
     gene            21570..21776
                     /locus_tag="PKM72_04845"
     CDS             21570..21776
                     /locus_tag="PKM72_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60145.1"
                     /translation="MFVWGPPIWVSEDLSNEQLSGPGLELEGSLNRLTAQAELAVQDS
                     DPVKSFLRRMSSPDMIEKTQTFDG"
     gene            21784..23145
                     /locus_tag="PKM72_04850"
     CDS             21784..23145
                     /locus_tag="PKM72_04850"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNP60146.1"
                     /translation="MYYVMYNVLLVLAFPIIIGLLLTKKRSQRGLWCRLGAVPLELRN
                     LQKPVIWIHAVSLGEVATIVPLLQAMKQQYPQWPLVVSTVTETGREVVIKRLEGVAVH
                     CYAPVDYLWAIDRYIRILQPRLFILVESEFWPNLLIRLQRHQVPICLVNGRISSRSFA
                     RYRWIKSMMNEVLTCLDLALMQSEHDAERIGYLGVNPNAIHVTGNMKFDQGLEQGQSA
                     DAPLSFRSLFTCHAAEMLIVAGSTHPQEEECLLEAYRKVVVQHPQAVLVMAPRHIERV
                     AKLEQVIRQYGLSCDRRSRLGQGPGEQAITHGPRVIVLDTRGELAFVYREACVTFVGG
                     TLVPVGGHNLLEPAQWGRPVLFGPHVDHCRDIAGRLLEVGGGIQIQNKEDLASQLIRL
                     IQDPSEAEAIGQRALEMVRTHRGVIARNLQWIDQLLNRKNSASLFSSIKGISSCTSQD
                     VLR"
     gene            23207..24307
                     /gene="lpxK"
                     /locus_tag="PKM72_04855"
     CDS             23207..24307
                     /gene="lpxK"
                     /locus_tag="PKM72_04855"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNP60147.1"
                     /translation="MNTTFPSFAVSPEGLWHWLHRRSPWVLRWCAVPYGAVGRLRRMA
                     YQHGWLPQHRLPKPVISVGNMTVGGTGKTPLVMWLAARLHEKGKRVAILSRGYGRQCP
                     TVNRLVSDGVSLKGDWRSVGDEAMLMAQKCPWAIVAVGTDRYQLGQWVLEQASCDCFL
                     LDDGFQHLSLFRDLDVLLFDATDVEGLSGILPAGRMREPLSAAKWATTIVFSRTEQKV
                     SVEALQHRIEQSVGKSIVPLRLETEPGMFTHIATGETQQAKAFKDRSSLLFSGIGNPC
                     SFRSSIVSCGLTVSEEVRYPDHFAYSEKDVEAIRLKMRRSGLDLAITTEKDALKIKEH
                     LTRDDAFWALDVRVRMTQGEDQLREQLHASCP"
     gene            24376..>24620
                     /locus_tag="PKM72_04860"
     CDS             24376..>24620
                     /locus_tag="PKM72_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60148.1"
                     /translation="MNILVRVPNWIGDAVMCLPALMDLRNHDPHAKVTVLARPIIGEL
                     LAGHPGVDEVIMYVHQGEHKGLLGLWELIQLVKSKQF"
BASE COUNT         5981 a         5336 c         6720 g         6583 t
ORIGIN      
        1 aagaaagttg gggttaaccc attatgattt caacactttc tgacagcgcc tctttcatca
       61 ctgtctcagt tggaaatgag atccgcgtgc gggctgagca gaaatccatc acgggggata
      121 ctcagcgcaa atcagagacg aagcaggtcc ttttattacc ggagccagct gaagattcaa
      181 tggcccatga accattgacc acagagcagg tgcaatcaac cgtggctcgc cttcaagaaa
      241 cattgaagca tattcaacct cgattagaaa tttctctgga aagggatata aatcaggtga
      301 tttttcgagt cgtggataaa gaatcaggtg accttatccg acaaatacct tcagagaaga
      361 tggtagatct tgcgcggtta ttttcggggc agtcagggct gcttgtggag gaaaacattt
      421 agtcaacttc tcaacccgtt tgccgttcat gagcccagtg actcagtcct tgagaaatcc
      481 aggttgtgta attcgattaa agtccgttga acgtttcgtc atgttaaaga aaatgacgag
      541 aggaatgtcg gatggctatt agtttcggtg ggcttgggaa tggggtagat tttggacctg
      601 ttgtcgatgc gctcgttcag gctgaacgct ttcccattga tgcccttacc cagaaaaaac
      661 ttcaggccca gaaaaaacaa accgaattgg gtttgttagg ggctaaatta ctaagctttc
      721 aaggtctggg gaacagtctt cgtaatcggg tcagctttaa caagaatcaa gtcaatgttg
      781 cctcagccag tgcccaaacc cctttgtcgg catctgtttc ttcgtcggca gcttctggta
      841 cgtatcaggt gactgtgaac cggttggcct ccgcacatca aattatttcc aaggcttcca
      901 cggcagtttc tgaatctgat gcggatattg ttagtggcgc atccagtaca tttcaatttc
      961 aggtgggtac aggttcaatt cacactgtaa atcttgatgc cgcgagtaca ttggatgatt
     1021 tacgttccgc gataaatgat ctgggtgccg gagtgagtgc ttccgttttg aatactggga
     1081 gtgaagcctc cccgcagttt cgtctcgtat tatcagcgaa tgagactggg cttgatcagg
     1141 ctatcacgat tgtggctgat gatacgaact tagatatggt gaccacagga gtggatacgt
     1201 ttcaagcggc tcaggactcc gaagttgtgc ttggggcgac ggacggcgag gtagggacca
     1261 ccgccattac gatcaatcgt tcgagcaata cacttaccga tgttgtgacc gggctcaccc
     1321 tcaacctcca agccgtggat gccagtaacc ctgtgacgat ctcggtgagt caggatcata
     1381 gtgctgtcaa gacagccatc tcggattttg tgaaaggata caatgaaata gtaacctttg
     1441 ttaatgaacg aacgttttat aatacagaaa ccaaggaacg tggtattttc gtcggtgaga
     1501 gtttagcccg aaatgttctt gatcgggtcc gagagtccgt cttttctcaa attggcgggt
     1561 taacgacgta cactggagct tcccaaattg ggtttgagac gcaaaccacg gatggaacga
     1621 ttaagctaaa tgaagcgact ttagattctg ccctcagtac aaatttttcc gcggtgcgtg
     1681 atttgtttgt caagaatgct accaccggca caaaggggat tgcggaacta gtcgtggatg
     1741 ccattgatgg attggatgac atcgacacgg gttccctcac gcgccgccaa aacgccttga
     1801 cgaaacaagt gaaggatttt acaagtcaaa ttacttttaa agaggaagcc ttgacgcgat
     1861 tcgagcagca acaacgtttg aagtttgcca acttagatgg gttgttggcc agattacaaa
     1921 gtcaattgaa acaaatgcag aacgcattcc ccacgacaaa tgggtaacga gaggatgttc
     1981 gtatgatggt tggtgctcag tcttatgcaa acacgcaaat ccaaactgcg tccaatgttc
     2041 aagtgattgt gttgctgtat gatggggcca tttcttccat gaaacttgca caagagggaa
     2101 tgtgtgcatt gaattatccg gataaggccc gattcctcga tcgtgcgctt cgagtgattg
     2161 gggaattatc ggctgccctt aatatggagg aaggtggtgg gatcgctaag gatttacgcc
     2221 ggatgtatga atacattcaa tttgagttga ctcaagccaa cctgaaaaat gaaccccgtc
     2281 gcttagaagg ccctattcgt tgtctttccc ttattcgaga agcctgggtg gaattggccg
     2341 ttcaaggagt aaagcctcag gcggtcggga tctaaacact gggattgtat gcctgaagtt
     2401 aactgccttg tcgaacagct caccaaagat gccattgagg cagccaaaaa tggtcaatgg
     2461 gatcaagtta ttgttctcta tgatcagcgt ctgtctcaag gaccacaaca atcccttacc
     2521 tcagaggccg tcaaatttct cgtggcatct gatcaatggt tggtcaagag ggttcgagaa
     2581 gcccaagccg cgatccagca acatctgaat gatatgcagg atcaacgacg caagttagag
     2641 gttctgaaac ggcagtgggg agccaactcc accatccagg tgcgacatct cctgacaatt
     2701 tgaaagttga gggtatgtat gagggttttc cgtgctttgc accatagaga attctccaaa
     2761 aaaccaaatc caatatctgt gcctgagatg agggaatttc cacgtcacta atttgacgga
     2821 cagagccaag ctaatcttca tgcttccatc aaattttttc atgtttcctt ctctcctaca
     2881 ttttcctgaa caaacagccg aaaagaaagg gaagcagtcc aggtataaca attgtgtgat
     2941 tgcatacttc aaagctgtga tttccccttt tagaaaatga catgcccggt tgttcactct
     3001 ccatctgaag gaacgacatc atgactcaac aatcggcatc cactgcaaac gctcatttgt
     3061 ctgtattgcc ggataataca gtgcaaaatg gaaaattatt acattcgctc tccaataaat
     3121 tactccctat tgtggtgttc tccgagttgg cgttacgtcg gtgcgaagat gaccagctga
     3181 agcgacaatt ggagaaaatt catggggccg ccgaacaagc cagggatatt cttgtggaaa
     3241 ttcggagcct ttacaacggg aagagctcct aatatgaact cagggttccc agattcttca
     3301 attgtactat tccccggttt atgtgtgacg gaatgactat tcttataaag gtgtcttatg
     3361 gatattgtgg cttctgagga tcgacgtcaa gattgccgag tggatgtgcc cgtatgtctg
     3421 tgggttgagg ttcctcttgc cttctcttat gaaacgattg atcttgatgg ggctgaattc
     3481 agccagtggc aatgggatga gttagctgtt tccccggatt tagtcaaggc catggtggat
     3541 gaaaaggatc tgaccattcg tgatcctttg ctcttacaga tggttacccg tattgactgg
     3601 atgttgacct caattctccg aacattggga aaagaaaaaa atctcaaggg ggctattcca
     3661 gaatttacga aagtgagtct atgcggttcc ggaataaaat tcttttccga agacacatat
     3721 ccccttgatt cctttctcgt cctacgcctc attctgcggc catttattcc tattcaggct
     3781 gttggcaaaa ttgttagagt tgattctacg cgaaaaggca aggaaccggg atttgagatt
     3841 gcggtggaat ttaccaaaat ttcttctgat gatcgcgaag cgattattcg acacacactt
     3901 cggtctcaag ccaccatgca acggcttcgg ctcaagcaac ctgtcgattc cgttcttgat
     3961 taaggcttcg ggaatttttt gctgtctgtt gtcctgtctc gtaatcgtgg caggatgtca
     4021 gagggcacct gacgttcccc tttctcctca ttcggtacct tcatcgtcta tttcctctcc
     4081 gccccctcgt cttcttggtc gaccgtatcg tgttgctatc ctgccttttg agaatatgac
     4141 ccggcacccc caactagatt ggttacgtcg tgggctacag gaaatgctgg ttagtgattt
     4201 agcgcaatgg tcggaacttg aggtggtatc acgtgatgtt cttggttcgg tcttacgtga
     4261 gcaatggttg caacagcgag ggttttcatc agatgagaat actgttggtc ttggacggtt
     4321 aaaaggagtg cggtatctca ttcaagggcg aatgtatgca caagaggagg cccttgccgt
     4381 cgatctgcag attgtcgacg tggagacggg ggtggttgtg ggggctgtca acgccaaggg
     4441 gttagagtcg gatattcctg gtttggagca agacattgtg actcgcgtcc ttggtatgtt
     4501 tgacccttct cttaatgtgg aaaccgggtc aatgtcagat cagatggcgg aaagctccca
     4561 gctgtcctcc aatcggcatc ttgagattga ccgggaaagg ggatccttgc catttttggg
     4621 atcagttcct tcacatgcag ttaatcaaat cgatgctctc ctatcattgg aaatgcttac
     4681 tcataagaga agggaggcgt attggttagc agaaactatt tggcatgagg gttgggtatc
     4741 tgaaattggg aaccccctcc accaggtttg gcaattcccg aatgcttctc tgagcaattt
     4801 accgattatg gctattccaa tttcactgtt tttctctcct catcggctcg cccatatttt
     4861 tgagcaaacg cagaagaccg aacccggtta tgggggatat gtggattctg atggattccg
     4921 gacgaccatc gatgaagctt ctggggctca gttattattt gttgaacatt ttcagaagcg
     4981 gcggcgtgta tttattcgtg caattaatga acaaggagat gtggtggcca ttttttccga
     5041 ttggggttgg cgaacggaac gtaaatttac aatgcaaggt actcaggggg tatctgtgcc
     5101 catgtggccg aacccgttta tcaccgggtt cgttaaattt cctgttgatt gggttgagcg
     5161 ggaagggcgc catgtgacgt ttgatagcgt ggtggttccg gtgccagacg agcaagttct
     5221 tgttttgctg gagccaattg gagagcttca aaaagaggaa atcgaaattt cctcacaaaa
     5281 tgatcaggag gatatttttc ttaaggaatt ggggaatcaa atcaaatcga attggacccc
     5341 ttcgataact gaaggacttc cagtgcgtgg gtatctgcca ggcaataaac ggacagcggt
     5401 gggtattgtc catgcccaag atggaaaaat tcgtgacatt caatttcagc attggcctga
     5461 ggatccgttt tttttgaaaa gcttacaaga tcttcaacta aagctattgg gggcttgtct
     5521 ggaatgtcgg aactccggtc aggttctccc ccctctccct gcttccttca aaacctttcg
     5581 tttacaactc acgctggtga aagacattca cgcccttcac ctgggcagtc gttcccacta
     5641 acctggaact attgagatcc ataagtggag tgactgttcc ggttctggtc actcaggatg
     5701 gttgtattaa ggtgggccaa tgccgtcgga gggtttcctc ggcggcgttg gcaagtatct
     5761 tgtcgatgga atgatcctgg ctgaaaactc gcagggtgat catccgagcg ggaagatatt
     5821 ggagaaattt ctgaaggcta tctttttcca ccgtgccttt ggcagggttc cagacaaata
     5881 tttgaaagtt ccccatgttg agcaaattaa tggggcgggg gtctttatga gcaaaatcta
     5941 ctcgaatatc ttgggaacga tattttgaag gtaacagtcg gcgcatccgt ttcagaaata
     6001 cctgaggttg aaaggatgat gctgccggct gaatgatggg gagcagcgtg ctgaatgccg
     6061 ttttccattt cacgtgtctt ccaataattt gactccactc taatcctagt tggcgtttgt
     6121 gcatggagcg agcgtgcttc caggaacgaa cggtttcgat gacatgccaa tcggttagat
     6181 ggaggtattg aggtaaggat tggaggggat tttttttgca caggatctcc atggtgggtt
     6241 gaaaaatttc ctgaagatga atgtcgatcg cccgtgtggt gcgatgatag taaacattgg
     6301 catagagata aagcctggcg tgtaaaaaca tttgtaacgc aggaagtcct gattggtgga
     6361 tcgtgagacc atgcgaggaa aagaaggtgt aatgaaggag tcgttctata tccaccggtc
     6421 caatggccac tccgcacata taggaatccc tgaggacgta atcacaattg tccggggtgt
     6481 aaattccgcc gagaaggggt tgtaaggcga taacccatgg tggaaggccc tttgtcgatt
     6541 tgtgaggatc cttaagaatg aggaaggcaa tatgatccgg atccaagtgc tctccgggag
     6601 caaaaggccc tgaaggactc cggcagatct ttttgatgat tggagccaaa tggtcacgaa
     6661 taatgtgttg gccgagcact tcatgcgtca ggtcaaattg atgcaggtaa tggtgatcaa
     6721 aaaaatggca gaacggtcca tgcccgatat catggagcaa ggccgtgata cgaataaatt
     6781 cttctaggaa atgacaagat ggtacttcgg agacggtttg gcaaagcgaa ggatagaggc
     6841 gaaccataaa ccgacctgcc aggtgcatgg ttcctagaga gtgctgaaaa cgggtatgtt
     6901 cggcgcccgg atagacccaa tgtgcactct gaagttggag aatataccga aggcgttgaa
     6961 cccagggaga atcaattaga tctttttcgg tttgttccgt aaactgtggt actgaatagg
     7021 gaacggtgat ggaaatataa ccgtgaatgg gatcggcaag aagcgaaacg ccatcaaatg
     7081 gagatgagaa tggttgtgat ccggccatgg gctaagagtt tatccctttg ctcaattcct
     7141 tccatgttag aaagtgctga ggtatcgcaa ccagcatggg gggagaactg ttccgctttt
     7201 cgatctgctg ctgcgccatg ttttttattc ttctaggaat ttttgaatcc ccggtatcgg
     7261 gtcatgaccc atcgcatgat gggataggcc atcaacgatc cgacaatcga aagggcacag
     7321 gccccaacaa aaaaaggtat gagatagggg gaaagcatgt caaacatatt gtccacgttc
     7381 atttgatccc actcaatcgt ggtagatgag gatgtgccta ccaacagcat tccggtatag
     7441 agactggcgg ctaaaattgg aacaagtgtc cagggattgt tgaccaatga ccccagaaag
     7501 agagctagaa tattcagccg gaaggcccaa gcgcagaaca cgacgctggc cgtgtggaaa
     7561 aaataatgtg gcgcgaacgc gataaacaca ccaagagaaa atgccaaggc tgtgcggtga
     7621 ggagtttcct gaaggtggag gacctgggtc agttgttgtc ggaccgattc aagcgttagc
     7681 atagacattt ccctgacaat ctcttactcg gaaaaatgca cataactttt tggtacaagt
     7741 tggtgagtcc taccgttttt tttcgtgatt ttaattcccg aacgacgaga ggtcatgacg
     7801 ccaatggcgg tcgcagaaat ccgtagtcgt ttaatcgctg attcaaggct gtgttgtttt
     7861 ttgggagata cagtaaacaa taattcgtat tcttctcctc cgtgtaggac ccaaggtagg
     7921 ggatctgcgc ccacctgtga agcataggcg aggagatgag gggacatggg gaggttggct
     7981 tccagaatga gggcacctac tcggctttgt tggcacaaat gcgtgatatc tcccgagagg
     8041 ccatcagata gatccaacgc cgccgaagcc aaacggcggg aagacagcaa tcgtcctaat
     8101 gcaatacgag gggtgggttg gagatggcgc ctaactaaaa acttcatggg ttgccgcagg
     8161 ttcgaaatct ttccgcggga accttgatgt gtcaggcatg ccaaacctgc agccgagttc
     8221 cccaatgatc cactgacata gataatgtct ccttccttag ccccacctcg tgttaatgca
     8281 tgaccgggct caacctgtcc cagtatggtg attgacagaa atagagaaga atgtgaggct
     8341 gaagtatctc cacccacgag ttgaacccca tgcgacttgc aaggaacgga aagcccacga
     8401 tacaggtctt tccagtcccc aaaccgcaca taggagggaa gggctatagc caccagtata
     8461 taggtgggag tggcccccat tgcggcaatg tcgctgagat tggctacggc agccttgtag
     8521 cctatatcat aaaatgaagc ggtatttttt gaaaaatgaa tgccttcaac gagtaggtcc
     8581 gtggatataa caatatgggt agatgaagga tgtgccagaa gtgccgcatc gtctcccatg
     8641 ccaatgagtg tcctcgaacc gggcggggaa aatccacgat tcagggcgcg gatgagttgg
     8701 aattccccta tttctgacac gggcgcctgg cggcgggaag gagacatgat aaaaatcagc
     8761 gtgtcttggg gacggtttgt cgtaaagggc gacgggtgac cgacgaggcc accttttttc
     8821 gacggccgga agaattattt ccatgagtta ttgccgagtt cagagtgtgt cgatttttag
     8881 ggcgaagggc gactttgaga acttccgaca tattctctac ataaataaac tgaaggcctc
     8941 gcaataaatg tttgggaatc tcattcaggt ctttttgatt tcttttaggc atgacaatgg
     9001 ttttgatatg ggcccgtttt gcggcgagaa ctttttcttt taacccgcca atggccagta
     9061 ttctgccgcg aagcgtgact tctcctgtca tcgccaggtc tcgtttgacg ggttggttgg
     9121 tgaggcagga cgcaagtgcc gtggcgatgg ttatgcctgc ggaaggcccg tctttgggta
     9181 tcgcgccggc aggtacgtga acgtgaatgt cttgtttggc gaagatgtca ggatcgattc
     9241 ccagtgactt ggcctgggaa cggatatacg tcagtgcggc atgggcagat tctttcatga
     9301 cttcgccgag atgccctgtt aaggttaaaa ggcctttacc cttgatggcc gaagtctcaa
     9361 tgtacaggac atctccaccg gtttctgtcc atgcgagtcc ggttgagatg ccgacttcat
     9421 ctttctcctg ctcctgatcc ggcacatatt tgggcacgcc tagatagtgg ttgaggtttc
     9481 gaggtgtgac ctgatgctgt tttatctttc cttctgcaat gtgtttggct acttttcgca
     9541 tcacattcgc gagttcgcgc tctagattgc gaacaccggc ctccctggta tattgagaga
     9601 tgatgcgtgc caagcacgga tcggtgagga gtaaatgctc ctccgtaatc ccgtgttctt
     9661 ctaactgtcg ggggatcaaa tagcgttgcg caatgccaag cttttcttcc tctgaatacc
     9721 ctggaatttc aatgatctcc attcggtccc gtagtgcgga gagaatgggg tccgttaaat
     9781 ttgcggtcgt aataaacatc actttactca ggtcaacggg taccccgaga tagtggtcca
     9841 taaaggcatg attctgttcg ggatcgagaa cctcaagcag ggccgcagac ggatccccgc
     9901 ggaaatctgt cccgatctta tcgacttcat ccaacatgaa cacggggtta ttggttccgg
     9961 cctgtttgat cccctgaaca atcctccctg gtaaagctcc gacatacgtt cggcgatgcc
    10021 cgcgaatttc ggcttcgtct cgtgttccac ctaaactcat gcgaacgaat tcccgaccca
    10081 tggcacgagc aattgattta cctaaggagg tttttccgac tccaggaggg cccacaaaac
    10141 acaagatggg ccctttcatg ttggctttga gtttgcggac tgcgagatat tccagaattc
    10201 gttccttgac ccgttccagg tcgtagtgat cctcatgcag cacctggctt gcctgtggaa
    10261 tgttgaggtt atcctcagaa tgatgattcc acggtaattc aaccatccat tccaaatacg
    10321 ttctgattgt cccggcctcg gccgtatcct ggtgcatttt ttctagacgt ttcagttgct
    10381 tctccgcctc tttcaggacc ttgtccggca tggtggcttc ttgaattttt cgacgaaatt
    10441 cagccacttc ttctgatcgg tcatcgagtt ctcccaactc tttttggatc gccttgagtt
    10501 gttcacgaag gaagtattcg cgttgcgtct tatccatttc acccttggct tcagcttgga
    10561 ttttctgttg catggacaaa acgtcttgct cgtttcccag gatttcattg acacgcctca
    10621 gacggagaag ggggtcttca atttcaagga ctccctgtgt ggtctcaaca ttgagcccaa
    10681 ggttagagga gataatgtct gccaggcgcc caggttcttc catgttttca atgacggtga
    10741 gaacatccgg catgaatatt tttcctaagc cgatgacttt acctaatgct tctcgggtgg
    10801 aacgcacggt ggcttctttt tctaatgaag gaagcgctgt ggacaattct gtgcaggttt
    10861 cgatgcgtgc agagaaaaat ggattggttt gggtgaattc ttgaatgcgg gctttggtga
    10921 gcccctgaat gagtatttta atacgggcat caggaagttt cagcatgcgc ataatgacac
    10981 cgaccgttcc cacttgataa aggtcttgag tctccggtac ttccacttct tgattttttt
    11041 gggtggttaa gaaaattaaa cggttggggg agaggagagc ggcttcaatg gccttaatag
    11101 aaatgttacg cccaacaaag aggggaagca ccatataggg aaagacgaca atatcccgta
    11161 caggcaggag tggtaattca tctgggatct ggtcaatgga aggtgtggga atctccgggt
    11221 agtcttcagt catcagtttc aatttctaac gatgaacgaa tagattaagc caagatatgg
    11281 caagaatggt gaatccgatt ttgcttaaat tcttcatgcc caaggagccc ctggcaaata
    11341 gcagggatca cttcggacgc aaggtgaacg gattaatggt cccggcttcc acagcacgtg
    11401 ccgaagatag tccgggatta gggatgggtc cctgagtgaa atccaatact tcgtttttta
    11461 agaatagcta gatgattttc aatgattttg atactaggag aaggtgaaaa atgttgtcaa
    11521 ggtatatccc cgttcaccga tcggaatgtc tctgggagta gggggtgcat agattgttgc
    11581 ctttacaagg aatcctgcca cgaatataat ctggcacctt gatcccaaca tgatgatttt
    11641 cttcccattt tccaggcttc ctccccattt tatcaccatc tcaaaggttt aactgttggg
    11701 gaacaaattg aaggggcaat tctgctgtcc cgctgaccat acaatttttc tgtgatacac
    11761 ccttcttgtt tttaccatcg cttcagagat gcttcatgga tctagtggtg tctcggtcac
    11821 ttggtcttgg gcaaaagaga aggcgttttc gaattttatc cttaccttct gttggctttt
    11881 ttctccttgt cagccttttc gttttggcaa tactttcgtt tgaggggcca gtgttggctc
    11941 aatcttccca atcccaaatt gaggccatta aggttgaagg aaaccagcac attgaggagc
    12001 aagccatttt agggaaatta tccatcaaac caggggatcc atttacccaa gagtctattc
    12061 gtgaacaaat tcagctcatt tatgggatgg gttttttcga agaagtcgaa gtctcaaccg
    12121 aagtcagggc cgaaggagtc ctcgtggttt ttggtgtaaa ggagaaaccg tttaccatcg
    12181 aagtggtgtt tgatggaaat gaagagttgt cagacgataa gcttaacgaa aaaaatgtca
    12241 taaaaaatca agtgttttta gataaagaac aggtaaaaat ttctgtagaa aatttccggc
    12301 agttgtatca cgacaagggg ttttaccatg ctcaaattat acctatcact gatatcgttg
    12361 agaacaatca agtccgattg acctattaca tcgaagaagg gacccaagct catattcgca
    12421 cgatcgtgtt tgaagggcga aatgtccttc agaagaagga attgacgtcg tttatggcca
    12481 atcgggaata ttcatggccc tggtcgattt tttcggatgc cggcattctc catcgcgatg
    12541 agcttcccaa tgatgtcgaa cggataaaag aagtgtatct caataaaggc tatttagatg
    12601 ttcaagtcgg gatgcctcga atggatttgg atgagacgaa ggaatggttt actctggtat
    12661 ttccgattgt cgaaggagag ccctatgtcg tgagtcaaat ccagtatacc ggaaatacac
    12721 ttttctcgga cgcagaattg cgggaaggtc tgatcattga gcctggtgaa gtctttcagc
    12781 gggccaaagt ccgtgatgag attacccgtc tgacggattt atatggcagt cgtggttacg
    12841 catttgccga agccaatcct tcggtagagc cggatccgcc cactcggagc actcgtattg
    12901 gtttctctat tgaggaaggc gaaatggtcc ggattcgtga tatccgcatt accgggaata
    12961 ataagactcg tgacaatgta atccggcgtg aattgcgggt ggatgaacgg gatatgatcg
    13021 attcccctgg aataaagcga agtttcgaac gcctcaataa tttgaatttt ttcgaaacag
    13081 tggaaatcct tccgaaacaa attgctccgg gagaggtgga cttggacgtc aaagtcaagg
    13141 agaaacctac cggctccttc agcattggtg gggggttcag tacgttagac caatttacgt
    13201 taattgcaga tatcaccgag gggaatttat tcgggcgggg gtatttagcc aggattcgag
    13261 ggcaggttgg agggcgacga actattggga cgattacctt tcgaaatccg gccatttttg
    13321 atacgttaac ctccggccaa gccgatctat tcagctctcg cacaaattac ctgacgtacc
    13381 tcgaaaaaaa gcagggtgcg aatttgacgt ttggtcgggg gttttccgaa tacttgacag
    13441 ggacctttac ccttgtggct gaaaagatca aaattgatga tgtggactct gatgcaaacg
    13501 atctgattaa agatcaggaa ggtagtcaat ccacaactgg atttcgtgcc agtttattcc
    13561 gggattcgcg tgattattac ctagatcccc ggcggggtac tcgaatcggt gtccgcgcga
    13621 gttttggaac cgatgtcctt ggaggatcaa ataattttta tagtatggcg tttgatgcga
    13681 tgaaatttac gcctctcccc ttttgggatt tgcgtcattc gattcgaggt cgtatcgggt
    13741 atggcgatgg atttaatggg tcagactttc ctattcctga gtttttttac gtgggaggta
    13801 ttaacactgt gcgggggttt aaatatggcc gtgccggacc ggtaaccgat ggaaacgatc
    13861 ctgaaggagg caataaacaa atcattttca ataatgattt aatttttccg gtgctgcctg
    13921 atgccaagtt gaacggggtt gtctttttcg actatggaag aggattttcc cagggagata
    13981 aattggatct tgatcttcga atggctgcgg gcatggaagt gcgatggatt tcaccatttg
    14041 ggcctcttcg ggcggcgtat ggccgtccta tagatggtcc aaaggatgac agaaagtggg
    14101 tatttgaatt ttctgttgga tcggtttttt agatgaattc tggaaaagaa gcatgaatgg
    14161 ttaggaggga tagagagaac tatatgagaa gtagtacaga tgttggaggt atgggcaagt
    14221 ggatggcttt tgggagttta gtcctcagtc ttgcctgggg atgcgcgggt atgggctcta
    14281 ataaaacatc ggatcttccg gtcggcgtcg ttgatcctca gcggatattg gctgagacgg
    14341 tcaaaggcaa gcggctgtcg gatgccctga atgccttcat gaaggatcga cagacgcttg
    14401 tggaattaga gcaaagggaa ttacggaagc tggaaagtga attaatggcg caaagtacgg
    14461 tattaagtca ggaggccagg gatcggaagg aagaacagtt cagggcaaaa atggctggat
    14521 atcaacaaaa agtcgccgat ttaaatagag aagttcaaga aaaacagcag gaattgcaaa
    14581 atgaatttcg tctccaggta caacgggttg taacggaagt tgccggtcaa cgcggcttgg
    14641 gtctggtctt ggagtacgga gtcaattcag gcacattgtt ttatgaagct ggactggata
    14701 tttctactga tgttattcag gcgctggatc gggagagcgg tgcgatcagt gtgccatagt
    14761 ccgtgggatg attcacggtc aggaggaaca atggtggccg aggagcaaaa agagaaaggg
    14821 gtcaattatg gttgagggcg agttggatat tctccacatt caggacttac ttccgcatag
    14881 atatcccttc ttattagtgg atcggatcct cgaaattcag gatgggacca ggattgtggg
    14941 tattaaaaac atcacggcca acgaacagtt ttttcaagga cacttccccg ggcgtcccat
    15001 tatgccaggc gtattactct tagaagtgat ggcacaagtt ggcggggtgt tggccagaaa
    15061 aacggccaag gagatcgggc gccctaccgt ttttctcaca ggaattgaaa aagccaagtt
    15121 ccgtcgaccg gttattcctg gtgatcaatt acgggttgag gttgaggtcg ttcgaagaaa
    15181 agatccattt tggaagatgg ccgggaaaat tctcgtcgag tcctccttag tctgtgaggc
    15241 tgaaatgacc gctatggtca aagttgaaga aagtgaggcc tagtcgagac caatagggtc
    15301 gttgcgcgag tctggctttg aaatcatttt caaccaaaaa gtgagaattt gatgaatatt
    15361 catcccacag caattgtcca tcccaaagcc acagtaggag aagatgtcgt catcgggccc
    15421 tattgcgtgg tgggcgaaca tgtcacgata ggagatcaaa cagaattagt ggcccatgtg
    15481 tgcgtggagg ggcatacgga aattggtcgg tgttgccggt tttatccatt tgtgtccatt
    15541 ggaacccctc cccagcattt gcaatataaa gatgagccca ccagggtctg tatcggggat
    15601 cataatattg ttcgtgagaa tgtgacgatt aatcggggta cggcctttgg aagcggagtg
    15661 accagaatcg ggcaccataa ctttttcatg gcctataccc atgtggctca tgattgtgag
    15721 ctgggcaatc atattgtgat ggccaatgcg gccaatttgg ccggccacgt ttcagtggga
    15781 aattctgtcg tcattggagg cctagtcggg gtgcaccaat atgtacggat cggtgaatat
    15841 gccatggtgg gagggtgttc ggccttagga cgggatgtcc cgccctttgt tcgtgcagcc
    15901 ggaggctatc gtgcccaaat gttcgggctg aatacgatcg ccttgagacg gcatgggttt
    15961 tccggagcac gtatgaatac cttaaaagcc gcatttgaac tcttattccg tcagggacat
    16021 cgtcttcaag aagccataaa gcttgctcgt aacgagtacg gtgaaagtac agacgtcatg
    16081 acgtttctca cgtttttaga aagtaccagt cgcgggatct gccaggtgtc ttctcgggag
    16141 ttggatgatg atgaggaata agttcgcggc cgtccggggt cgtagggtat gatttctcct
    16201 gaacaggatg agcggattgg tctgattgcg ggaaacggac gctttccgat tatttttgcc
    16261 gacaatgttc gacgattagg gttttcggtt tccgcgatcg cacatgtggg tgagaccctg
    16321 ccggaacttg aatctcatgt cgatcgcatt cattggctaa agatcgggca atttagcaag
    16381 gcattggcgg cattaaaggg ggatggcatc cgtcaggccg tcatgttggg tggcattcat
    16441 aaaacgcatg tctttaccgc acttcgaccc gacctccgaa ccttggcgat ttttagccga
    16501 ttaaagcact ggaaggacga tgcgattctc agagcggttg ctggagaatt agagcgggaa
    16561 gggattcaga ttcgagattc tacctttggc cttgaaggga ttctggcggg agagggcaat
    16621 ctctcctaca ggaaaccaac aagtaaagag tgggacgata ttcagtttgg gtgggatacg
    16681 ctggaaacct tgggaaagtt agatattggg caatgtgtgg tggtgaaaaa tcgggtgatt
    16741 gtcgctgttg aagccgttga gggaacggat ggaacgatta ctcgtggtgg aacccttggt
    16801 ggaaagggta ccgtggtggt caaacgaacc aaaccgcacc aggaccttcg gtttgatttg
    16861 ccggccgtcg gccctcaaac cattcagtca atggtcgccg taaaagcttc ggttcttgct
    16921 attgaagccg ggcgcaccgt cgtccttgat cgagatgaga tgtttgtgaa cgccaaatca
    16981 gcaggtatcg cgatcgttgg aatcgcccct atggaccctt tacactctcc tgccgggtct
    17041 tctgatgctc caccgtaagg ggatgttttc ccactcgtgg tgtttcgggt ggtgtaacac
    17101 gaaccgctgt cattgttctc ctattcagcg accatctatc cctacatgac acaatcaata
    17161 cgagttgggg taattggtgt tggtcatttg ggccgacacc atgctcgcat ttatggaatt
    17221 ctgccttctg tgacattagt aggggtctct gataccgata ctttacgcgg gcatatggtt
    17281 gcggatgaat gtggcgtacg atatttccag gatgtggacg agctcctgtc tttggtcgat
    17341 gcggtgagtg tggcggtccc gacctcggtg catggtgttg tggtcacgaa ctgtctgcaa
    17401 cgcgggtgcc atgtgctcgt tgaaaagccg attgccagcc atgtgtggga aggtgagcag
    17461 ttggtcgagc tggccaaagt ccgtgggagg atattgcagg ttgggcatat tgaacggttt
    17521 aatccgattg tagaagtgat gcgtcctctg gtgagtcagc ccggctttat tgaatgtcac
    17581 cgtctaagtc cttttcagcc tagagggaca gatgtcgatg tagtacggga tctcatgatt
    17641 catgatttag acctcttgtt atcgttgggg ttggggccca ttatgcaggt cgaagcgaga
    17701 ggaatgccgg tttttacgga tctgccggat attgccaatg tgcgaattct gtttgaaagc
    17761 gggtgcttgg ccaatttgac tgcaagccgt atctcgacag gacgacttcg aaagatcaga
    17821 gtgtatcaac gtgatcgcta tctctccgtt gattttggag gaaaagaggc tgtcatgaac
    17881 accaggagaa gtttggaggg tggaacgttt gaagttgaaa cgcaacatat caagggggat
    17941 gaaggcgatg ccctgactcg tgaattggac tgctttattc aatcgattct gggaaatccg
    18001 tccgcccgag gtgtctcagg ggaagaaggg gtggaagccc tgagagtcgc gactcaagtt
    18061 gtctcgctca ttcaacaaca tgccgggcac tcgccacgat aatcggtgtc cgtgggaatt
    18121 ttccgccctg agtgatctgt tagagtctcg atgccaaaaa ttttcttagt gaccggtgaa
    18181 acgtccggcg atattcacgg agcgcactta gccctggcac ttcgggaact gaatcccgct
    18241 gttgagttaa tcggtgtggg tggaagtcgg atgttggaag ccggtgtctc gcttcttccc
    18301 ggtgtcaaac gggtcgatgc catgggcgtc ccgggtatcc ggcagctcct acagggatgg
    18361 aagacgttac gaacgcttgc ttgttatctt aaacatgaac ggtttgatgt cattatcctt
    18421 attgatagtc cagggttgaa tcttcgtttg gccaaggcta ttgcaaagag gtcacacaaa
    18481 atcatctatt atattgcacc tcaggtgtgg gcatggggaa gccgtcgatt aaaagttatt
    18541 cgaaaagtaa tcagccatgt attagcgatt cttccattcg aagaagccta tttccaaaaa
    18601 gccggcatcc cctgcacgta tgtcgggcat ccattattgg acgaactaaa gcctgcatac
    18661 gatattgttc atgaacgtca acaactaggg ttagagagag aagatctggt aatcggtctg
    18721 ctgcctggga gtcgtgtccg ggaagtccgg gaggtgttgc cggtctttct gcgttcggtt
    18781 gaacagattc ggagtcaata ccctcgcatt cagatccttc ttgcccaagc gcattcactg
    18841 tccgattctg tggtgaatga aattcttgga catgtcggac aacaggtgaa ggtggtcaaa
    18901 ggccggtcca atgaggtgat ggcggcctca aatcttctgt tggtgacatc cgggacggcg
    18961 acgttgcaat cggcattgat tggaacgccg atggtcgtgg tctaccgggc atcacccttg
    19021 acctatcata ttggcaaatg tctggttaaa attccctata ttagtttagt gaatatttta
    19081 gccggtcaag aaattgttcc agagctgatt caggatcgta tgactcccga ccgtattgca
    19141 catgaagcct taaaaatttt acaagacgca cgccgtcaaa acgacatgac acaggctttc
    19201 caggctattc ggaattccct gggggaggcg ggtgcgtcaa aacgggctgc cgcatttatc
    19261 cttgctgagg cgatcgtatg aagaccttat ggcgaatctt gtcttatctg aaggagtacc
    19321 gcctgcgctt gattggtgcg tttgtctgtt ctgccggagt ggcaggatta tcagcggtct
    19381 atgcctggct tgtccaaccg gttctggatg gaatcttcat tgagcgcaat caagagatgt
    19441 tggtcatcct tccgttggct gtcctggggg tcgcgatttt gaaagcgtca tttgcgtatg
    19501 gacaagggta tctcatgagc tatgtggggc actggcttgt gtccgatatt cgccaacaat
    19561 tgtttattca tattgtgcgg ctgcctattc gttttcacga tgcgaatgcg tcaggacggt
    19621 tgatggccag ggtgattagc gatgtcaacg aaatggcgaa tgccattccc agtgtgttga
    19681 aggatatctt tcaacagggg ctgacgtttg tggcgatgct gtcagtggtg ttctatcaaa
    19741 attggaaact cgcgactatt gtgttggttg tcttaccctt ctcctcattg gtgttggtcc
    19801 gggttggccg gcggattcgt aaactttcaa aacgtggcca agagtcgatt ggaaaaatgg
    19861 cttcggtatt aaaagaagcc ttttccggca tcaagattgt gaaggcttat ggtcaggaag
    19921 agaaggaagc tcaacgattt tctttgacaa atcaagcatt tcgaacagca aaagtgaagt
    19981 cgtcccaaac ttcagcgatg gcctctcctc ttcttgaagt catcggggtc tgcggtgtgg
    20041 cctttattat atggtatggg ggaggattgg tgattgccgg agagatgaag ccgggggagt
    20101 tcttttcatt cctggctgcc atgttcatgg cgtatgcccc cctcagaaaa atgtccggag
    20161 ccaatgagtc ggttcaacgt gcgcttgcgg gagctcagcg agtgtttcat gttctcgact
    20221 tagaaagcga gatagcgaaa gacgaaggga aaaaatctct tccacccata gcgaaagatt
    20281 tggagttttc cgctgtcaat tttagctatg agggttctga agagcccgcc ttgcacgaga
    20341 tcaatttcac catcaaggtg ggggaggtgg tggcgttcgt cggggccagt ggaagtggaa
    20401 aaaccactct cgtgagtttg gtgccacggt tttatcgtcc gacggaagga accgtgaaaa
    20461 tcgatggtca ggatattcgc ctggtggatc gatcctctct gagacggcag attggcatcg
    20521 tgtctcaaga aactgtgtta ttcgatgata cgatcagaaa taatatcgcc tatggctgtc
    20581 tggatgcgag tgaggagtcg gttattgatg cggctcgcgc cgcttttgcc tgggagttta
    20641 ttgagcgact cccccaggga ctggacacgc tggttgggga gaatggactg cgattatcgg
    20701 gtggacaacg ccaacgactc gctattgcca gggcgatttt gcgggatccc ccgattctga
    20761 tacttgacga ggcgacatcc gctctggatt cggaatcgga aaagctggtc cagaaagcgc
    20821 tggtcaatct ggtgaaagcc agaacgacct tgatcattgc ccatcgattg tccacagttc
    20881 aacatgccga tcgtattgtg gtcatgaatc gtggaagaat tgaagagatt ggtacccatt
    20941 cggccttgct tcagcaagga ggcctctata ctcgactgta tcaaacgcaa tttttattca
    21001 cccaacccga accgttgcca acgatctcct aaatcgaatg acgccgttcc tatgctgaat
    21061 tggatgaaac tgtcggtcgc gcctcctttg gcagcgcgaa tcattcgcct cttgggactg
    21121 acgatgcgca tatctaccgt gggaggggaa gctgttgacg agttgtatcg ccagggccga
    21181 catatcataa ttgccttttg gcatggccga cagttgatga tgccgttagc ctaccgaggg
    21241 cagcaggcct caatcctgat aagccaacat cgggatggag aaattattgc gcgcatcatg
    21301 ggttattttg gatttctgtc cattcgcgga tcgagcaccc gagggggact tcgggccacc
    21361 aggcaattat tgaaggccgg gcgaaatggc ggggatctgg tggtgacacc cgatggcccg
    21421 aagggaccag cctgcaccgt acaaccggga gtgatttatc tggccaaagc gacggggttg
    21481 ccaattatcc ctctgacctt tgcctgctca aaaaaaaagt ctttacgagt tgggatcgct
    21541 tccagattcc ctatcccggt ggggtagggt tgtttgtttg gggaccaccc atctgggtca
    21601 gcgaggatct ttccaacgag cagctctccg gtccagggtt ggaactggaa gggtcgttaa
    21661 atagactcac cgctcaggcc gaattggcag ttcaggattc cgacccggtc aaatcgtttc
    21721 tccgcaggat gtcctccccc gacatgatcg agaaaacaca gacattcgac gggtgaccaa
    21781 ctcatgtatt atgtgatgta taatgtgctg ctggtgctgg cctttcccat tattatcgga
    21841 ctcctgctga caaaaaaacg cagtcaacgc ggtctatggt gccggcttgg tgcggttcct
    21901 ctcgaattgc ggaatcttca gaaacccgtc atttggatcc atgcggtctc cttgggagaa
    21961 gtggcgacca ttgttcctct gctccaggcg atgaaacagc agtatcctca atggccgttg
    22021 gtcgtgtcga ctgtgacaga gaccggccgg gaagtcgtga tcaagcgttt ggaaggtgtg
    22081 gctgttcatt gttatgctcc cgtcgattat ttgtgggcga ttgaccggta tatccgcatc
    22141 ctccagcctc ggctctttat tttggtcgaa tcagaattct ggccgaatct gttgatccgt
    22201 cttcaacggc atcaggtgcc aatttgtctc gtcaacggga gaatctcttc acgatctttt
    22261 gccaggtatc gctggattaa aagcatgatg aacgaggtgt tgacctgtct ggatcttgcc
    22321 ttgatgcaat cggaacacga tgctgagcgg atcggctact tgggggtcaa cccgaatgcc
    22381 attcatgtca ccgggaatat gaagttcgat caagggcttg agcaaggcca gtctgccgat
    22441 gcccctctct ctttcaggtc actcttcacg tgtcatgctg ctgagatgct gattgtggcc
    22501 gggagtacgc accctcagga agaagagtgt ttattggagg cgtatcggaa ggtggttgtg
    22561 caacaccccc aggcggtgct ggtgatggct ccacgacaca tcgaacgtgt ggctaaactt
    22621 gaacaggtta ttcggcagta tggactttcc tgtgaccgtc gcagtcggtt gggacagggt
    22681 cccggagagc aggctattac tcatggtcct cgcgtcattg tgttagatac caggggggaa
    22741 ttggcttttg tctatcggga ggcctgtgtc acgtttgtgg gggggacact ggttcccgtt
    22801 ggaggacata atttgttgga gcctgcccaa tggggtcgcc ccgtcctgtt cggtccccat
    22861 gtcgatcatt gccgggacat tgccgggcgg cttcttgaag tagggggagg aatacagata
    22921 cagaacaaag aggatttagc atcgcaattg attcgtctga tccaggatcc ttcagaggcc
    22981 gaggccattg gccaaagggc gttggaaatg gtccggaccc atcggggggt gatcgccagg
    23041 aatcttcaat ggattgacca attgttgaac aggaaaaatt cggcctctct cttctcgtcg
    23101 ataaagggga tttcttcgtg cacgtctcaa gatgtgctcc gataagtttc cgttccgtga
    23161 aaggttgaaa tgagggggaa aatatgatct acgccgtgtg ttctttgtga acacgacgtt
    23221 tccttcgttt gctgtgagtc ctgaagggct ctggcattgg ttgcaccgtc gtagcccatg
    23281 ggtcctccgg tggtgtgctg ttccctatgg tgcagttggc cgcctgcgga ggatggcgta
    23341 tcaacatggc tggcttcctc aacatcgatt accaaaaccg gtgatcagtg tggggaatat
    23401 gaccgtgggg ggaaccggta agactcccct tgtgatgtgg ttggccgccc gactccatga
    23461 gaaagggaag cgggtggcaa ttctgagccg tgggtatgga aggcaatgcc caacagtaaa
    23521 cagactggtc tccgatggcg tgtcgctgaa gggtgactgg cgatcggtag gggatgaagc
    23581 catgttgatg gctcagaagt gtccatgggc gattgtggca gtgggaaccg atcgctatca
    23641 actggggcag tgggtgttgg agcaggcctc atgcgattgc tttcttcttg atgatggctt
    23701 ccagcatctt tctttgtttc gagacctgga cgtgctctta ttcgatgcga ccgatgttga
    23761 gggtttaagc ggaatcttgc cggccggccg catgcgtgaa ccgctctccg cagcaaaatg
    23821 ggctaccacc attgtcttca gtcgaacgga acaaaaggta tcggtggaag cacttcagca
    23881 tcgaatcgaa caaagcgtgg gaaaatccat tgtccccttg cgtctggaaa ccgaacctgg
    23941 aatgttcacg catattgcga caggtgaaac ccaacaggcc aaggccttca aggacagatc
    24001 ctccttgctc tttagcggaa taggcaatcc atgctccttt cgatcgagta tcgtttcctg
    24061 tgggttgacg gtgagtgagg aggttcggta ccccgatcat tttgcctatt cggaaaaaga
    24121 tgttgaagcc attcgtctga agatgcgacg ttcagggctt gatctcgcga tcacaacgga
    24181 aaaagatgct ctgaaaatta aagagcatct gacacgcgat gatgcgtttt gggcgcttga
    24241 tgtacgagtg cggatgactc aaggcgaaga ccagttacgt gagcaactcc atgcgagttg
    24301 tccataggac tcagtggtgg ttggtcaact tgcatctagc cgatcagttc atgatggagg
    24361 ggctatcgtt tttcgatgaa cattctcgtc cgtgttccca actggatcgg agatgccgtg
    24421 atgtgccttc cggctctcat ggatttacgg aatcatgacc cgcatgccaa ggtgacggta
    24481 ttggccagac cgatcatcgg agaattgttg gcagggcatc cgggtgtgga tgaggtgata
    24541 atgtatgttc atcaaggaga acacaagggc ctcctcgggc tctgggagct cattcaactt
    24601 gtgaagagca agcaatttga
//
LOCUS       DAORAQ010000031        24007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_397_length_24007_cov_75.816174, whole genome shotgun sequence.
ACCESSION   DAORAQ010000031 DAORAQ010000000
VERSION     DAORAQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 24007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24007
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_24007_cov_75.816174"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKM72_04865"
     CDS             <1..816
                     /locus_tag="PKM72_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNP60149.1"
                     /translation="LGAALNVHYSYLNQPRVCQGSSDSMRGSYLGPRFSNEEIETSLQ
                     QLEARYERVEDSDLYRRVAEYLAEGKVIGWLQGHMEFGPRALGGRSILGDPRSEKMQS
                     VMNLKIKYRESFRPFAPSVLRERVSEYFEMDTDSPYMLLVAPVAEKRRRAVQPDLQKL
                     WGIDLLNVPKSDIPAVTHVDYSARVQTVSPDTNPRYYQLLQEFEKQTGCAVLINTSFN
                     VRGEPIVCTPEDAYRCFMRTEMDVLVLEDCLVLKEHQKPVPKDEAWMNEFELD"
     gene            835..1239
                     /locus_tag="PKM72_04870"
     CDS             835..1239
                     /locus_tag="PKM72_04870"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNP60150.1"
                     /translation="MTSTIYQPTAKDLRSFGLLMGGVFLIVALWPLVIHGEGIRVWAS
                     LVAGTFGAMGMVYPKGLGPLHRVWMKIGEKLGWINSRIILSLMFFGMFTPMAFVMGVL
                     GKRPLQLGYDPKANSYRIPKKARSADHVLKPF"
     gene            1254..1406
                     /locus_tag="PKM72_04875"
     CDS             1254..1406
                     /locus_tag="PKM72_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNP60151.1"
                     /translation="MTGFLGELWDFMRERKKFWLAPIIIMLLLLGGLIILSEGSAVAP
                     FIYTLF"
     gene            complement(1560..1868)
                     /locus_tag="PKM72_04880"
     CDS             complement(1560..1868)
                     /locus_tag="PKM72_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60152.1"
                     /translation="MNRKLCAINVIGSVVFGSALALGNPGMLPDHPGYPMGDAKSPVT
                     GQSVANDPGQSHPSRDESLQQASGFHDSHTMNPGKEERPNIVPPGGSKAMNTKSDSGK
                     "
     gene            3138..4112
                     /locus_tag="PKM72_04885"
     CDS             3138..4112
                     /locus_tag="PKM72_04885"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNP60153.1"
                     /translation="MSGSEHEQLSRLRAFLNQQVIGQEGLTLRLLIALMADGHLLVEG
                     APGLAKTTAIKALASGIEADFHRIQFTPDLLPADITGTDIYRPNDGSFRFQPGPIFHN
                     LVLADEINRAPAKVQAALLEAMGEHQVTIGRTSYPLPELFLVMATQNPIEQEGTYPLP
                     EAQLDRFLLYVRVKYPDVEGERKIAELVRRQARAIPGDGMPPPTPVPQKVILAARRQM
                     WDIYFAPALEEYIVQLVMATREPGPYSSMLKRWIRFGASPRATIGMERCARAHAWLEG
                     RDYVSPEDIQAVAHDVLRHRVLLTFEAEAEGIRTDQVISEILNHVAVP"
     gene            4096..5103
                     /locus_tag="PKM72_04890"
     CDS             4096..5103
                     /locus_tag="PKM72_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNP60154.1"
                     /translation="MWPFRKTPSPSEFRPNRPAGFQGVEVRLADLINMRHQTGVLGIN
                     TRKRVHTLLAGGERSPFKGRGMDFEESRRYQPGDDVRLMDWRVMARTHEPYLKVFREE
                     RERPVFFVVDNRKGMRFGTKVAFKSVIAAHAAALLGWASQERGDRIGAVVFSDVDHVE
                     LRPRGGRTGVLQLLNLLAQDSVHPSEVNEQQNPSASPLQLALNRVQATAKPGSLIFLL
                     SDFRDWDQQAKQTLIRLGGHQDVVVIFMYDKLEQEPPPAGQYPVTDGIRTGILNTGSA
                     STIQSYSACFKERYEDVRTLCLTRGIGFISLGTHDDILFQLRGGLQDIGHRRSAQHSM
                     A"
     gene            5119..5616
                     /locus_tag="PKM72_04895"
     CDS             5119..5616
                     /locus_tag="PKM72_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HNP60155.1"
                     /translation="MPTSGSLLQELRDVHLPAPISLWPPAPGWWITLGLVMMGGMVFL
                     WILRNRRRTQARRLAMNELGAIKQHYDTHRDDQWLVQRLSTIVRRYAMVTFPRTEVAG
                     LVGVSWLQFLDRSGRTNEFTAGVGHLLSSGPYQQQAVVSGNELMQLVEHWIQHVTPPK
                     GRERT"
     gene            5613..6662
                     /locus_tag="PKM72_04900"
     CDS             5613..6662
                     /locus_tag="PKM72_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNP60156.1"
                     /translation="MMTFAWPWALAFVLFPWMVQRWAPSVSNSSGRAMKVPHFEDIMS
                     LQSSQLIEANRSRRSTLFWVGIIIWSALLLAAARPQWSGDPVGLPTSGRDLMLAVDIS
                     GSMKIPDFSVNGREVTRLEVVKAAAGEFIARRTGDRVGLIVFGSQAYVQTPLTFDRDT
                     VKAMMTETEIGLAGQETAIGDAIGLAVKRLKEQPAGSRVLVLLTDGANTAGEVSPTQA
                     AALAEEQGIRIYAIGVGADRMEIASFFGTQTVNPSRDLDEDTLRLLAQRTGGLYLRAK
                     DTEGLRKVYEELDRLEPATTETELFRPTTELYIWPLTFALALSCVTVMSFIWKKEWFL
                     RPRWKAEGIPARAGR"
     gene            6664..8577
                     /locus_tag="PKM72_04905"
     CDS             6664..8577
                     /locus_tag="PKM72_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNP60157.1"
                     /translation="MSMFHFLRPEWLWGLLPLFGLFLLAARSGRTAQIWESVCDTHLL
                     PHLLVGTEGATHRMPLVLLGLGWTVAVFALAGPVWSQLPQPVFRAESALVMVLDLSRS
                     MDVQDVTPSRLTRAKHKILDMLKARKEGQTALVVYAGESFVVSPLTDDVNTMVPLVQS
                     LETELMPSQGSRTDLALQKAHELLQHVGQAKGEVLLLTDGEGDPATLKVAEELRRQGK
                     RVSVLGVGTVDGAPIPEIGGFLKDQDGAVVIPKLDIPSLQAIARVGGGRYATVTADDQ
                     DINRVFPSSMPTRALSSSTSEQRTTDLWREEGPWLVLLLLVLALPAFRPGWLGMLLAF
                     LLIPQISEAFSWENLWVRPDQQGIKALEREAPEEAAALFQDPGWKGIAHYRAGKYQEA
                     EEAFSIVDTPEGHYNRGNALARLGRYEEALASYQTALTQQPDHEDAKHNLEIIKQLLD
                     KPSSADQGGEESQESDDSSAKQKNNRNETGVEGEKSEEASDSTHKQPDSEKVDSQNGK
                     QEKSDQTVGSGADPATTSNQSNGNQHASEENIEREAQGVDPLKTEQAKNPLSPPSAME
                     ENSEDQSEHSGVTSAVDAAQIRERQQSEQVLQQWLRRIPDDPGGLLRRKFLLEHQRRV
                     EAGGSTFSQGKRW"
     gene            8619..10379
                     /locus_tag="PKM72_04910"
     CDS             8619..10379
                     /locus_tag="PKM72_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNP60158.1"
                     /translation="MMVLSTPVSAQTVRAYVDRNPVMADETFQLIVETDQTASGESPE
                     WDSLDADFDVLGTTQSQQTSIINMQTTSLVRWIATLAPKRTGPVTIPPIAVGPHQTGP
                     IQLMVNEPNYSSGANEERDIFLEADVDSHFPHLQGQILLTLRLVSAISVQEGRLDEPE
                     IDWGIVERVGKDASYEATRSGRRYHITERRYVITPQQSGAHVIPEVLFSGTVQDGRTR
                     GRLFEELFGNRSGSLGGNPFKTSQSIHRRSPKIELTVKDLPPDLNGRFWLPAKALTLT
                     ETWSGDTDTLQIGDSLTRTIVMRAKGQRGEQLPEVTMPSQPIVKIYPDKAKTQTDFDG
                     KWVVGSREEKFVFVSTHPGTVTLPAIHVPWWNIETSRWEEANLPAKVLTVLGTAPPGS
                     SAQVPTSVQAPSVTSIEGDHQVNAQMPANIGISDRAEQLPWTWITGGCLLLWLVTLAG
                     WWRERQKRNGRSEEPAKKDDEKFESEKKAIQVVKTACFEQSAKKTRDALLQWASFKEE
                     GRPCRSLRLVERILSQPISDQNAISEAIWNLDRTLYTNSTEQETWDGRQFWETVKPAI
                     TAKAPKSHKHAKELPPLYLH"
     gene            10476..11423
                     /locus_tag="PKM72_04915"
     CDS             10476..11423
                     /locus_tag="PKM72_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP60159.1"
                     /translation="MVEIKNLRKAFGPIVAVDGVSFTVGKGEVLGFLGPNGAGKSTTM
                     KMITGFLTPTSGTVHICGHNIEDQPIEAKMRLGYLPEGAPAYQDMTPASFLTFIGEMR
                     GYRGATLKRTVSETVEKVNLQSVMNQSIETLSKGFKRRVGLAQAILHDPDILILDEPT
                     DGLDPNQKHEVRTLIRAMAQEKAIILSTHILEEVHALCSRAMIIAKGKVVFDGTPAEL
                     EGKSPAHNTVLGTIAGVDPLVLHHHLMTVKGVKKIEELDRDGDLVKFRIYPDDGQWIL
                     PDIGHCVREQGWEVRELYPDRGQLDEVFRLLTTPKQTIS"
     gene            11583..12320
                     /locus_tag="PKM72_04920"
     CDS             11583..12320
                     /locus_tag="PKM72_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNP60160.1"
                     /translation="MGRIGVIFQRELAGYFATPIAAVFIVIFLLLSGAFTFYLGNYFA
                     RGQADLVPFFDFHPWLYLVLIPALAMRLWAEERRSGTIELLLTLPVTMWEAVAGKFLA
                     AWCFTGIALALTFPMWLTVNLLGDPDNGVILASYLGSLLMAGGFLAIGSCISALTKNQ
                     VIAFVVSVVISLGFILSGFPLVLELFSGWAPQFLLNAISSFSFLTHFQSISKGVLDLR
                     DILFFLSLIAFWLFATATVIDMKKAES"
     gene            12365..14293
                     /locus_tag="PKM72_04925"
     CDS             12365..14293
                     /locus_tag="PKM72_04925"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNP60161.1"
                     /translation="MMNRKILTGSGLLMAAVLFGVFNMVSNAAFSSARFDLTEHDLYT
                     LSDGTRNVLKNLEEPITLRFYLSQKLATGLPGIKSYATRVREMLEEYAQVAGDQLYLQ
                     VMDPEPFSEEEDRAVAYGLQGIPLDNGSTQFYFGLAGTSSTDELEVIPFFQPEREEFL
                     EYDLTKMIHTLANPKKKVLGLLSTLPIDGSGGMPFMPQQGGSQPWLILSHIEQMFEVK
                     KIEPTAVAIPEEISVLMIVHPKSLSEATLYAIDQYVLGGGHAMIFVDPLAESDSGGGN
                     PMNPMGGGGPRDSNMPKLFEAWGVELVKGQVLGDLPLAKKVQVQQQSRLQVVDYPVWI
                     DFRQEHFSDKDIVTAQVPSITVASAGIIRKKGETGTTVEALIQSDEAAMQIEASRLSA
                     MPDVGGLLNSYRPEGEKFIVAARVTGTVKTAFPEGKPKEVPTKDENPDTAPETESQTK
                     DHLNESTEPINVIVVADTDILQDRFWVQVQNFFGQRIGIPNSGNGTFVTNALDNLTGS
                     NDLISVRSRAGYSRPFTLLRVLQQEAEQQFRQKEQVLQEQLKATERKIQELQSQKPEG
                     NSMILSVEQQEAMGQFRKELLQVRKELRAVQHELGKNIERVESWVKFINIGLVPLLIG
                     IAGVWISSSGIRKKRPKA"
     gene            14357..15547
                     /locus_tag="PKM72_04930"
     CDS             14357..15547
                     /locus_tag="PKM72_04930"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNP60162.1"
                     /translation="MKAKTLGILAAITIVGIVVAVFVNQEPASQLPGSGELLFPSLLS
                     VVNNVGEVVVETKDQTVTLVREEKTWRVKEKAGYRADVEKVKQTLIGLAELRILEPKT
                     KNPELYDRLGLQDKEQEDSLSTTVTLKTANNPEAAVVILGNQRPAKGNPRMSEIYVRK
                     PGDPQTWLTIGNLPLEKVSGEWLDTEITALTTKRVHRVTVTHPDGETLLVLKEKPDDL
                     DFQLDSVPAGSKVASQFNVNNVVGTLVQLPLEDVKKEEEVNFQNHTGVTVVLETFDGL
                     RLHVQTTKQEDKIFGKFSAEFDQKLVQPVEDTPLSEKGETVQAQDSAQDEMDLGSPEG
                     KQPDEEQGKRYSEEESILKKPEEVQGEVEAFNQRVQGWAYQLPTFRVENFSKAKKDLL
                     ATIP"
     gene            complement(15608..16249)
                     /locus_tag="PKM72_04935"
     CDS             complement(15608..16249)
                     /locus_tag="PKM72_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60163.1"
                     /translation="MMFAGIIAILLFLCLIIGDWFQFTTLKSWACRYGCPLAHRQKAL
                     DSHPAHPISHAFHKSDALPLPHGIARWVEDKQVIVIRPYYQLFSLSFRTAWPLKGTID
                     VSSAGNQLLLHLSKRIPWSSAILTLLWLLFVIGGTLTFVVLFILDGGMSTIGGVFWVV
                     GITALGCLVLAGGLILLSFAYRLEDSRLMQVHQELLDALTAPPLKGSDKPENA"
     gene            16337..16413
                     /locus_tag="PKM72_04940"
     tRNA            16337..16413
                     /locus_tag="PKM72_04940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16371..16373,aa:Met,seq:cat)
     gene            16843..17958
                     /locus_tag="PKM72_04945"
     CDS             16843..17958
                     /locus_tag="PKM72_04945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP60164.1"
                     /translation="MNKISQDDKVQKDSGTLSSIQGITISIVVPVYNGGEAFRQCLMS
                     LVALQPPPMEIIVVADGESDGSWQVAEQFGAQVIRLPTTSGGPAQPRNIGALQAKGEY
                     LFFVDADVCVHPDTLRQVAETFHCNPDLTALIGSYDDTPAEKNFLSQYKNLFHHYVHQ
                     HAQKEASTFWGACGAIRRDIFLEIGGFDEKYRRPSIEDIELGYRVKKAGHRIQLCKDI
                     HVKHLKRWGIRSMLKADFFYRAIPWTELILRDRRFTNDLNIRHSERLCVVLTYGLVGA
                     LLGAIWWIPLLPVAGLIMVSLVIINAPLYRFFQKKRGLWFALQSIPWHWLYYLYSGLA
                     FAIGLARHVFCGDNRKESTKPALESDSFDAIKKSGGQ"
     gene            17958..19796
                     /locus_tag="PKM72_04950"
     CDS             17958..19796
                     /locus_tag="PKM72_04950"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNP60165.1"
                     /translation="MASHPVVVIGAGPAGLTAAYELGKNSSPSLILEAGKQVGGISQT
                     VNYRDFRFDIGGHRFFSKVPMVTELWNEILGDNFLLRPRISRIHYNQHFFDYPLKATN
                     ALAGLGVVEALLVCFSYAKTKVFPGAQEDNFEQWVINRFGYRLYQIFFKTYTEKVWGI
                     SCTEISADWAAQRIKNLSLKEAVRNALLGQRGGKKGEIVTSLIEQFHYPRLGPGMMWE
                     RCEELVAGFGSQTLKGIKVERIRHRHGRVDCVSGRASSGEHVEFEGSDFVSTMPLREL
                     IEAMDPQPPAKVLEAALGLRYRDYLTVVLVVNCEDVFPDNWIYIHSPEVKMGRIQNYK
                     NWSPYMVPDPSRTSLGLEYFLWDKDDEWTWSNERLIEFGTRECAQLGLINPRDVEDGT
                     VVRMEKAYPVYDHHYQDHVRTIREYLETFSNLQTIGRNGLHRYNNQDHSMVTGVYAAR
                     NIMGESHHDVWAVNTEKEYHEEGRAASVNAGDRMVPMRVQENVDELPMENEEELIEVV
                     FSKLDPVALGVAVGTVSGLLILIGTAILVLKGGPVVGPNLSLLGNFLFGFQVTWSGSI
                     VGFLEGGIGGFAIGYSGASLRNWSMKAFAKFMLWREEMTRRRNLLD"
     gene            19821..20129
                     /locus_tag="PKM72_04955"
     CDS             19821..20129
                     /locus_tag="PKM72_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60166.1"
                     /translation="MGTDFKSNEELSRVVAKIQAGVLAVVCALMGGLGLFFMTAWLLL
                     EGGPQVGSHLQLLSNYFIGYSVTWKGSLVGLFYGALTGGILGWTIGFIYNRVVGIRGG
                     "
     gene            20827..21615
                     /locus_tag="PKM72_04960"
     CDS             20827..21615
                     /locus_tag="PKM72_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP60167.1"
                     /translation="MINEGAGYWDSIADEWKKTRPDQLWRKHSDTVNQSLLLRWLPEQ
                     PSPRLLKTDTFDEAVGGGLIQFLKTRAHKVVGMDLSFQNVELACPGDMSIRGACADVR
                     HLPFGEESFDVVVSNSTLDHFLTLDEIIVSLRELHRVLRKNGQLILTLDNPANPIIAA
                     RNILPFKLLNRLGILPYYVGATCGPWRLQKLLREIGFEVREATAVLHCPRIFAVLVAK
                     LLCSCAKGSVQQRFLLWLEAFEHLSRWPTRYVTGNFIAVRAEKK"
     gene            21682..23079
                     /locus_tag="PKM72_04965"
     CDS             21682..23079
                     /locus_tag="PKM72_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60168.1"
                     /translation="MRELGWSTGHFYCSLVLLNQNRCKRRDAAPKRMMLLDGWKTFRL
                     IRQALSSSRWSLVDLRQVQEERIRVLLNHAYHNVPLYRSLYKEAGYHPEEFRSLDDIG
                     EIPILQKDRLKQASPEEVVVQGIDPNRCRIVETSGSTGTPLRIFLGSIDQQWQRVVAW
                     RILFEHGFRWTDRTLEIRMTFGRQFFVQRLGIAPKDWCSILDPPEFWAQRMVKTRPDV
                     IVAGAGTLHALAETVQTLGFKLPAPRLIISDSETLSPATRQLVRCALGRDPVDVYGLV
                     ELSNFAWECERHSGFHVSADSHIVEVAAASGEPGPIITTALGMWTMPMIRYETGDLAE
                     VETTSCPCGRTLPLLRRIYGRRVDSVALQDGRRIFWPFFHEVLGRYDELHQWRILQDR
                     LNEVQLQIVVTNNAQDLLSRIESDLQAVLPGEIHLQVERVERILLSPGEKTRMIISKV
                     GTAIEEQPASGRQGL"
     gene            23110..>24007
                     /locus_tag="PKM72_04970"
     CDS             23110..>24007
                     /locus_tag="PKM72_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60169.1"
                     /translation="MDLRSNVTRSPAHLRSLQDHLFRAAAIHAYRNVPFYRRVWDEAR
                     VDVQAIRGIQDLESIPIITAPMVREAAKLGELLARGVDPSSCTYLDTSGSSGVALRVW
                     KRPLEERVRRAVGLRIWFEHGFRWRHHTAQFQIKPGPSHMLQRFGISRKTWISTEQSI
                     EDQLAQLLEAKPDVVVGTATALRRIARASEAAQVILKQPRVVFCAGELVDAETRQLVT
                     RVFNRKPVGLYGQTEVGYIAWQCEQRGSFHVNADTHFVEVLRDGIPAHPGDLGTIVVT
                     DLRARTMPLIRYNTADLAIAASG"
BASE COUNT         6037 a         5382 c         6587 g         6001 t
ORIGIN      
        1 ttgggggcgg cgttaaatgt gcattacagc tatctgaatc agcctcgggt atgccagggc
       61 tctagcgact cgatgcgagg tagttattta gggcctcgct tttctaacga ggaaatagaa
      121 accagccttc aacagcttga ggccaggtat gagcgcgtgg aggattcaga cctgtatcgt
      181 cgagtggcgg agtatttggc tgagggaaag gtcatcggat ggttacaggg gcacatggaa
      241 tttggtcctc gggctttagg gggacgaagt atcttgggtg accctcgtag cgagaaaatg
      301 caatcggtca tgaatttaaa gattaaatat cgggaatcct tccgtccctt tgcaccatcg
      361 gttttaagag aacgagtgtc agagtatttt gagatggata ccgatagtcc ttacatgtta
      421 ctggtggccc cggttgccga gaagcgacga cgagcggtac aaccggatct acaaaaactt
      481 tgggggattg atctgctgaa tgttcccaaa tcggatattc cagcagtgac tcatgtggat
      541 tattctgcca gggttcagac ggtgtcacct gataccaatc ctcgctacta tcaattgttg
      601 caagagtttg aaaaacaaac cgggtgtgcg gtgttgatca atacctcatt caatgttcga
      661 ggcgaaccga ttgtctgcac gcctgaagat gcctaccggt gttttatgcg gactgagatg
      721 gacgtcctgg tgctggaaga ttgtctcgtc ttgaaggaac atcaaaaacc ggtgcctaag
      781 gatgaagcgt ggatgaatga gtttgaattg gattaagcgg gaaagaataa tgacatgaca
      841 tcaacgattt atcaaccaac tgcaaaggat cttcgctcgt ttggcttgtt aatgggcgga
      901 gtctttttga tagtagcgtt atggccattg gtcattcatg gagaaggcat tcgagtatgg
      961 gcaagtcttg ttgccgggac ctttggggca atgggaatgg tttatccaaa aggtctgggt
     1021 cccttacatc gagtctggat gaaaatcggg gaaaaattag gatggatcaa ttcccgaata
     1081 atattaagcc taatgttctt tggaatgttt actcccatgg cgttcgtcat gggagtatta
     1141 ggtaaacgac cattgcaact tggttatgat cccaaagcga acagctatcg cattcccaaa
     1201 aaggccaggt ccgcagacca tgtgctgaaa cccttctaaa ggaggaaaaa cagatgacag
     1261 gttttcttgg ggaattgtgg gactttatgc gggaacgaaa aaagttctgg cttgccccca
     1321 tcatcatcat gctcttattg ctcggcggcc ttataatcct aagtgagggt tcagccgttg
     1381 cccccttcat ctataccttg ttctaaatcg aaggggagga atggagctac taggaaacct
     1441 gattgagtgg caggcaaaag tttgcataga aaataaaaaa aggagggagc tttaagctcc
     1501 ctccttttca gaccccctgg tgctcccgat tgttcgggga cggtttgcag acttataccc
     1561 tacttgccgg aatcactctt tgtgttcatg gcctttgatc ccccaggcgg cacaatattc
     1621 gggcgttctt cctttccagg attcatggta tgtgaatcat gaaatccgga agcttgttgg
     1681 agcgattcat cccggctggg gtgagattga cccgggtcat tggctaccga ctggcccgtc
     1741 accggggatt tggcgtctcc catgggataa cccggatggt ccggcagcat tccaggattt
     1801 cccagggcta aggcacttcc aaatacgaca ctccctatta cgttaattgc gcatagttta
     1861 cggttcatgt tcatccctcc tttttgttcg atacgatcgg gaatcagggg tacagaacca
     1921 gaacctagtt tattttttat cgaagacctc tttggttgtc ttccaatttt ggccccctcc
     1981 cgttttttta attgtgggta ttaccgattt cgtttgaaca ggcaattggg ccaaagatga
     2041 ttcagtgctt cacttggcta tggcatacct tcgtttggtt tgagggttta tacaggaagt
     2101 aaatcttaaa tcaatcctgg gatccccctt cttctcactt ttgctaaagc caggaagaac
     2161 gtagtcccca ttcacacccg cgcgagactt aatctaggct aaatcttgct taacttctcg
     2221 tgaagagggt gaggaggatt tcttgatggt gtgcaataca gcataaggtc accgcattga
     2281 ttgacgggat atttaggtcc aagccttgat gttaaaactg gactatggca atgggcaagt
     2341 ggcgcaaatt agggaagaac tgttgagaga ggtaaatgaa agttactctg ggaaagtagt
     2401 ggtatttcat tagtttttga aatgtaaacc caaaaactaa aaaaccgcca aatgaaaagg
     2461 atgacgatca caaaaagatc gaattcatag gaattatgaa attagaagaa gaaaatatta
     2521 aacataacca tttcgtgaat agaaaacctg agggttgtcg acaggcactg tgcaaaattt
     2581 ttctgggatt atgcatgtta atgagcacta cgtgggcgtg gggtagttcc actcatattc
     2641 agtttagttg agagatttgc gccgtctttt actcctcaac aggcttgatt gacctcggaa
     2701 atctccattt atgcgagaat ccaacggcaa ctcctcatac gatgaggcat aacggttgcg
     2761 gagaggatgg agcatgtgca tttgattcaa gtgccatttg ctcacctgac acaattaaaa
     2821 ttcctggtcg ttttccaggg caattcacta ccattcattt cacactgctc cccgatatgc
     2881 gggttatggt cgaagttggc atccctttcg cattctcctc catctgattc tcaaagaatt
     2941 ctacaagggg gaaagggatc tgtggttttt tggtaagaat atcgtttccc cgcattacct
     3001 cttgactctc aaaaatggta atttattatt aataagagga cttcagaccc ccgaagggtg
     3061 aagttatcct gtgccattca ataagggact cattagtaat ttgactacag gaacccaccg
     3121 gtttcacatc aagacacatg tctggatctg aacacgagca gctatcaagg cttcgagcct
     3181 tcctgaatca acaggtaatt ggacaagagg gcctcactct caggctattg attgctctga
     3241 tggctgatgg acatcttctg gttgagggtg cgccaggttt agccaaaact actgccatta
     3301 aagcgttggc ttctggaatt gaggccgatt ttcaccggat tcagtttacc ccggatttac
     3361 ttccagctga tattaccggg accgatatct atcgtcccaa tgacggctcc tttcgcttcc
     3421 aaccagggcc gatttttcac aatctggtcc tcgccgatga aatcaatcga gcgccggcca
     3481 aggtgcaagc agctttatta gaggccatgg gcgaacatca agtaacgatc gggcggacca
     3541 gttatccttt gcccgagttg tttttagtca tggctacaca aaaccccatt gagcaggaag
     3601 ggacctatcc gttgccggaa gcccaattgg atcggtttct tctctatgtc cgggtgaagt
     3661 accccgatgt ggaaggagag cgaaagattg ccgagcttgt tcgtcgacag gcgagggcga
     3721 ttcctgggga tggaatgccc ccccccaccc cggtgcctca aaaagtcatt ctggcggctc
     3781 gtcggcagat gtgggatata tatttcgcgc cggcactcga agaatatatc gtccaattag
     3841 tcatggccac gcgtgagccc ggcccctata gctccatgct gaagcggtgg atccggtttg
     3901 gagccagtcc ccgggcgacc atcggaatgg agcggtgtgc ccgggcgcat gcctggttgg
     3961 aaggacgtga ttatgtttcg ccggaagata tacaggccgt ggcccatgat gtgttgcgcc
     4021 accgagtatt actgactttt gaagctgaag cggaggggat caggaccgat caggtgatct
     4081 cagaaatttt gaatcatgtg gccgttccgt aaaacgccat caccatcaga attcagaccg
     4141 aaccgtcctg caggatttca gggagtagag gttcggctcg ccgatctcat caatatgcgt
     4201 caccagacag gggtattggg aatcaatacg cggaaacggg tgcataccct gttggctggc
     4261 ggagagcgtt ctccattcaa gggcagaggc atggattttg aggagtctcg ccggtatcaa
     4321 ccgggcgatg atgtgcgcct catggattgg cgggtaatgg cgagaactca tgaaccctat
     4381 ctcaaggtct ttcgcgaaga gagggagcga cccgtcttct tcgtagtgga taatagaaag
     4441 ggaatgcgat ttgggaccaa agtggccttt aagtcagtca tagcagcgca tgctgccgca
     4501 ttattgggtt gggcatcgca agagcgagga gaccggattg gggcggtggt tttttcagat
     4561 gtggaccatg tggaactcag acccagagga ggaagaaccg gggtccttca acttctcaac
     4621 ctcctcgctc aagatagtgt gcacccgtca gaggtgaatg aacagcagaa tccctctgcc
     4681 tcacctcttc agttggcctt aaatcgagtc caggcaaccg cgaagcccgg aagcctcatt
     4741 tttcttttga gtgattttcg ggattgggac cagcaggcca aacagaccct tattcggttg
     4801 ggcggacacc aggatgtggt ggtgatattc atgtatgata aattggagca agagccgcca
     4861 cctgccggcc aatatcctgt aacggacggt atacgaacgg gaatattgaa tacaggatct
     4921 gccagtacaa tccaatccta ttcagcgtgc ttcaaggaac gttatgaaga tgtccggacg
     4981 ctatgcctga cccgtggaat cggattcatt tcattgggaa cccacgacga tattcttttc
     5041 caattacggg gtgggttaca ggatatcggg catcgccgaa gcgcccaaca cagcatggca
     5101 taacgggaat gggctagcat gccgacatca ggttctctcc tacaagagct tcgagatgtg
     5161 catttgccag ccccgatttc tctgtggcct ccagccccgg gttggtggat taccttaggt
     5221 ttggtcatga tgggaggcat ggtgttcctg tggattctga ggaatcgacg tcggacacag
     5281 gcgcgtcgac ttgcaatgaa tgaacttggt gcgatcaaac agcactatga cactcaccga
     5341 gatgatcaat ggctggttca acgactttca accattgtcc gccgctatgc catggtaact
     5401 tttcctcgaa cagaggtagc aggattagta ggagtatcat ggctgcagtt tttagatcgg
     5461 tcgggtcgga ctaatgaatt cactgccgga gttggccatc tgttgagttc agggccatac
     5521 cagcagcagg cggtcgtgtc gggaaacgag ctgatgcaat tggtcgaaca ttggattcag
     5581 cacgtgactc cccctaaagg gagggagagg acatgatgac gtttgcctgg ccgtgggcct
     5641 tggcgtttgt tttgtttccc tggatggtgc agcgctgggc accttctgtg tccaattcat
     5701 ccggtcgcgc catgaaagtg ccgcactttg aagacatcat gagtcttcaa tccagccagt
     5761 tgattgaagc gaatcgttcc aggcgctcaa cattgttttg ggtggggata atcatctggt
     5821 ctgctctgct tctggcggca gcccgtcccc agtggagtgg tgaccctgtc gggttgccaa
     5881 ccagtgggcg agatctgatg ttggcagtcg atatttcagg aagcatgaaa attccagatt
     5941 tttcggtgaa cgggcgggaa gtcacccgct tggaagttgt gaaggctgca gccggggagt
     6001 tcatcgctcg ccgaactggc gatcgagtgg ggttgattgt atttggctcc caggcctacg
     6061 tgcagacacc tctgacattt gatcgggaca cggtaaaggc tatgatgacg gaaacagaaa
     6121 ttggcctggc ggggcaggaa accgcgattg gggatgcgat cggccttgcc gtgaaacgac
     6181 tcaaggaaca accggcggga agccgggtat tggtgctatt aaccgatgga gccaatactg
     6241 cgggagaagt gtcacccaca caagcggcgg cgttggctga ggaacaaggt attcgaatct
     6301 atgccattgg ggtcggtgcc gatcgaatgg aaattgcatc attttttggc acacagactg
     6361 tgaatccttc tcgcgatttg gatgaagata cccttcgcct tcttgcccaa cggaccggag
     6421 ggttgtactt acgtgcgaaa gatactgagg gactcaggaa ggtgtatgaa gaattggatc
     6481 gtctcgagcc ggcgacaact gaaacggaac tgtttcgacc gacgaccgaa ttatatatat
     6541 ggccgttaac atttgccttg gccctttcgt gtgtaacggt catgtctttc atctggaaaa
     6601 aggaatggtt tctcagacca agatggaagg cggaagggat cccagcacga gcaggacgat
     6661 aaaatgtcca tgtttcattt tttacgaccg gaatggttat ggggattgct tcccttgttc
     6721 gggctctttc tgctggcagc caggagtggg agaacagctc agatatggga gtcggtgtgt
     6781 gatacccatt tactccctca tttattggtg gggacggagg gagcgacgca tcgcatgcct
     6841 ctcgtgcttc tcggattagg ttggactgtg gcggtttttg ctcttgccgg gcccgtctgg
     6901 tcccaattac cccagcctgt gtttcgagca gaatcggcgt tggtgatggt tttggattta
     6961 tcccgttcaa tggatgtgca ggatgtgact ccctccaggt tgacccgagc caaacacaaa
     7021 attcttgata tgctcaaggc caggaaggaa gggcaaaccg ctctggtggt ctatgccggt
     7081 gaatctttcg tggtgtcgcc actgactgat gatgtcaaca ccatggtgcc tcttgtgcaa
     7141 tccctcgaga cagaactcat gccatcccag ggtagccgta ctgatctcgc tttgcagaaa
     7201 gcccatgaac tccttcaaca cgtcgggcag gcaaagggag aggtgctatt actgacggac
     7261 ggcgagggag atcctgcaac attgaaggtg gctgaagagt tacgccgtca agggaagcgt
     7321 gtgtctgtgc ttggtgttgg gacggtcgat ggagccccta ttcccgaaat aggcggattc
     7381 ctcaaagatc aggatggagc ggtggttatt cccaaattag atattccgtc tcttcaagct
     7441 atcgctcgag taggaggagg gcgttatgcc acggtgaccg cggacgatca ggatatcaat
     7501 cgagtttttc cttccagtat gcccacgagg gcgctatcct cttcaacatc tgaacagcga
     7561 acaacggatc tgtggcgaga ggagggaccc tggctggtcc tcctgctcct ggtattggca
     7621 ttgcccgcat ttcgaccggg atggttagga atgctgctgg cattcctgct tattcctcag
     7681 atcagcgagg ctttttcctg ggaaaatctc tgggttcgtc ccgatcaaca ggggattaag
     7741 gcattagagc gtgaggcccc ggaagaagcg gcagccttgt tccaagaccc aggttggaag
     7801 ggcatagccc attatcgggc agggaaatat caggaagcag aagaagcctt ttccatagtt
     7861 gatacgccgg aggggcatta caatcgaggc aatgccttgg cccgtctcgg tcgttatgaa
     7921 gaagccctgg cttcctatca aacagctctc actcagcagc cggatcacga agatgccaaa
     7981 cataatctag aaatcatcaa gcagctcctg gataaaccat catctgcgga tcagggagga
     8041 gaagaatctc aggaatcgga tgactcctct gcaaaacaga aaaataatag aaatgaaacc
     8101 ggagttgaag gagaaaaatc agaggaggct tcagactcaa cccacaaaca acccgattcg
     8161 gaaaaagttg actctcaaaa cggaaaacaa gagaaatcag accagacggt cggatccggg
     8221 gcggatcctg ccacgacctc taaccagtcg aatggtaatc aacacgcttc tgaagaaaat
     8281 attgagagag aggcgcaagg ggtagatcct ttaaagacgg aacaagctaa aaatccgctg
     8341 tcccctccat cggcaatgga agaaaattcg gaagatcagt cggagcattc gggagtgact
     8401 tctgccgtgg atgcggccca aatacgggaa aggcagcagt ctgaacaggt tctccaacaa
     8461 tggcttcgcc gtatccctga tgatcccggc ggattgttac gccgaaagtt tcttttggag
     8521 catcaacgcc gtgtagaggc tggagggtcc actttttctc aaggaaaacg ctggtgagaa
     8581 ttttgagatg cctattggta atggttgttt gccttggaat gatggtgctt agcactcctg
     8641 tttcggccca aacggtcagg gcctatgtgg atcggaatcc ggtcatggca gatgagacct
     8701 tccagctcat cgtggagacg gatcagactg cttccggtga atcgccagag tgggatagct
     8761 tagacgcgga cttcgatgta ttgggcacga cccaaagtca acaaacttcg atcatcaata
     8821 tgcaaaccac ttcattggtt cgatggattg cgacgctggc gccgaagcga acaggaccag
     8881 tgacgattcc tcctatcgcc gtgggtcctc atcagactgg ccccattcaa ctgatggtca
     8941 atgagccgaa ttacagcagc ggcgcgaatg aggagcgaga tattttcctc gaagcggatg
     9001 tggactcaca ttttccccat cttcaaggac agattcttct gacgcttcgt ttggttagtg
     9061 cgatttccgt tcaggaaggc cgattagatg aaccggaaat tgattggggc atagtggagc
     9121 gcgtcggaaa ggatgcttcc tacgaagcca ctcgcagcgg gcggcggtat catatcaccg
     9181 agcgtcggta tgtgatcacc ccgcaacaaa gcggggctca tgttattccc gaggtcctct
     9241 tttctggaac ggtgcaggat ggacgaaccc gtgggaggct ctttgaggaa ttatttggca
     9301 atcggtccgg gagtttgggg ggaaatcctt tcaaaacctc acaatcgatt cataggcgaa
     9361 gtccgaagat tgagctcacg gtcaaagatc ttccgccaga tttgaatggg cgtttttggc
     9421 tgccggcgaa agcactcacc ctcaccgaaa catggtcggg tgataccgat acacttcaaa
     9481 taggggattc ccttactcga accatcgtga tgcgtgcaaa aggtcagcgt ggggagcaac
     9541 tgcctgaagt gacaatgccc tcacaaccca tcgtaaagat ctatccggac aaggccaaaa
     9601 cccaaacaga ttttgatggg aaatgggtag tagggtccag ggaagaaaaa ttcgtgtttg
     9661 tgtcgactca tcctggcaca gtgacgttgc ccgctattca tgttccctgg tggaacattg
     9721 agacttcccg ctgggaagaa gccaacctcc ccgctaaggt tctgacggtt ctcggcacgg
     9781 ctccaccagg atcctcagca caggttccca catcggttca ggccccgtct gttacttcaa
     9841 tagagggtga tcatcaagtg aatgcccaaa tgccggctaa catcggcatc tctgatcgtg
     9901 ccgagcaatt accatggacc tggataacag ggggatgctt gctgttgtgg ttggtgaccc
     9961 tggcgggctg gtggcgagag cgtcaaaagc ggaatggacg gagtgaggag ccagcgaaaa
    10021 aagatgatga gaaatttgaa tcggaaaaaa aagctattca agtcgtcaag accgcatgct
    10081 ttgaacagtc agcaaaaaaa acgcgcgatg cgctactgca atgggcttca ttcaaggaag
    10141 aagggcgtcc ctgtcggagc ctgagattgg ttgagcgtat attgagtcag cctatttctg
    10201 atcagaacgc gatttccgag gccatttgga accttgatcg gactttgtat actaactcca
    10261 cagaacaaga gacttgggat ggtcggcagt tttgggaaac agtcaagccg gccataacag
    10321 ccaaggcgcc aaaatctcac aaacacgcaa aagagttgcc cccgttgtat ctccattgag
    10381 tggagaggga gctgatcgaa tcaggtagcg agagcacgac tgtgcgacag atcatttcat
    10441 aaaattattg agataaagag ggtagaatgg aaatcatggt tgaaattaaa aacttgcgaa
    10501 aagcatttgg gccgattgtt gcggtggatg gtgtgtcatt taccgtagga aaaggggaag
    10561 tgctgggttt cctagggccg aacggagcag gtaaatccac aaccatgaaa atgatcacgg
    10621 gttttcttac gccgacgagc ggcacggtgc atatttgtgg gcataatatt gaagatcaac
    10681 ccatcgaagc taaaatgcgt ttaggctatc tgccggaggg ggctccggct tatcaggata
    10741 tgactccagc ctcgtttctc acatttatcg gagagatgcg gggatatcgt ggagcaacgc
    10801 ttaagcgaac cgtttcggaa acggtcgaga aggtgaattt gcaatcggtg atgaaccaat
    10861 cgatcgaaac cctatctaaa gggtttaagc gtcgagtggg attggctcag gcgatccttc
    10921 atgacccgga cattttaatt ttagatgaac ccaccgatgg tttggatccc aatcaaaagc
    10981 atgaagttcg aacactgatt cgggctatgg cccaggagaa ggccattatt ctctctacgc
    11041 atatcttgga ggaagtgcat gcactctgtt cccgcgccat gatcattgcg aaggggaaag
    11101 tggtgtttga tggcactcca gcagaattgg aaggaaagtc tccggctcac aatacggtcc
    11161 tgggcacgat tgccggtgtg gatcccttgg tcctacatca tcacttgatg acggtgaaag
    11221 gggtcaaaaa gattgaggag ttggatcgtg acggtgactt ggtgaaattt cgtatttacc
    11281 ccgatgacgg gcagtggatt cttccggata ttggacattg tgtccgggaa caggggtggg
    11341 aagtgaggga actgtatccc gaccgcgggc agttagatga ggtgtttcgt ttgttgacga
    11401 ctcccaagca aacaatctcc taatacgcga ttgttctgga aggcggggta ctccgagcag
    11461 gtgaggcgcg tagccggtgt acaccctcaa atcaccagga gcaaatctcc atgcgcttct
    11521 gagagaccag tggcgaaaaa ttaaagggac tgaaagaagt attcgtaagt gaaggaggaa
    11581 ttgtgggacg aattggagtg atttttcaac gtgaattggc cgggtatttt gccacaccta
    11641 tcgcggcagt attcatcgtc atatttttgt tactcagtgg agccttcaca ttttatctgg
    11701 ggaattactt tgcgcggggt caggccgatc tggtgccgtt ttttgatttt catccctggc
    11761 tctatcttgt gttgatccct gcattggcga tgcgcctatg ggcggaagag cgtcgttccg
    11821 gaacgattga gctgttgctg accctgccgg tcaccatgtg ggaggccgtg gcagggaagt
    11881 ttttagccgc atggtgtttc acaggtattg ccttggccct gacctttccc atgtggctca
    11941 cggtgaatct gttgggcgac ccggataatg gcgttatcct tgccagctac cttggcagtc
    12001 tgttgatggc cggggggttt ttagccatcg gctcctgcat ctccgctctg accaaaaacc
    12061 aagtgattgc ctttgtggtc agcgtcgtca tttctttggg attcatcttg agtgggttcc
    12121 ctctcgttct agaactattc agtgggtggg cgccacaatt tctgcttaat gccattagct
    12181 cattcagctt tttgacgcat tttcaatcga tcagcaaagg tgtcctggat ttacgggata
    12241 ttctcttttt tctgtcgctg attgcattct ggctatttgc gacggctacg gttattgata
    12301 tgaaaaaggc cgaatcataa aggaatgcaa gggatcgggt gtgaactttt gttaatgggg
    12361 agtgatgatg aatagaaaaa tactcacggg aagcggtctt ctcatggccg ccgtcctgtt
    12421 cggtgtcttc aatatggtga gtaacgcggc gttcagttcc gcacggttcg atctgacaga
    12481 acatgacctg tatacattgt ccgatggcac cagaaatgtt cttaagaatt tggaggaacc
    12541 tatcacactg cggttttatc tctctcaaaa gctggcaact ggtttgccag gaattaaaag
    12601 ttacgcgacg cgagtccggg aaatgttaga ggaatacgca caagttgctg gagatcaatt
    12661 gtacctgcag gtgatggacc cggagccgtt ttcagaggaa gaggatcgag cggtagcata
    12721 cggtttgcaa ggcattccgt tggataatgg cagtacgcag ttttattttg gattggccgg
    12781 caccagctcc accgatgagt tggaagtgat tccatttttt caaccggagc gagaagagtt
    12841 tttggaatat gatctgacta agatgattca cacgctggcc aatcccaaga aaaaggttct
    12901 tggcctattg agtacactac ccattgatgg aagtgggggc atgccgttta tgccccaaca
    12961 aggaggcagc caaccctggc tcatactttc tcacatcgaa caaatgtttg aagtgaaaaa
    13021 gatcgaacca acagcggtcg ccattcccga agagattagt gtcttaatga ttgtgcatcc
    13081 taaatcgctg agtgaggcga ccctttatgc gatcgatcag tatgtgttgg gaggtgggca
    13141 tgccatgatt tttgtggatc cccttgccga atcggatagt ggaggcggaa atcccatgaa
    13201 cccgatggga ggaggaggtc cccgggattc aaatatgcct aaattgtttg aagcctgggg
    13261 tgttgaattg gtgaaagggc aggtgttggg agatttgcca ttggccaaaa aggtgcaagt
    13321 gcaacagcaa agccggctac aggtggtgga ttacccagtc tggattgatt tccggcagga
    13381 gcatttcagt gacaaggata tcgtgacggc tcaagtgccg tcgatcacgg tggcatcagc
    13441 aggaatcatt cgaaagaaag gggagaccgg gaccacagtc gaggcgctca tccaatcaga
    13501 tgaagccgcg atgcagattg aggcatcccg cttgtcagcc atgccggatg tcggtggatt
    13561 actcaatagt tatcgtcccg aaggggagaa gtttatcgtg gccgccagag taaccggaac
    13621 cgtgaaaacg gcttttcctg agggaaaacc aaaagaggtg ccaaccaagg atgagaatcc
    13681 ggatacggct ccagaaaccg aaagccagac caaagaccac cttaacgaat cgacggagcc
    13741 tatcaacgtc attgtggtgg cagatacgga tattttacag gaccggtttt gggttcaagt
    13801 tcaaaatttc ttcgggcaac ggattggtat tcccaactcc ggcaatggca cgtttgtcac
    13861 gaacgccttg gataatttga cgggaagcaa tgatctcatc agcgtcagaa gtcgcgccgg
    13921 atattcccgg ccattcacac ttctgcgagt gttgcaacaa gaagccgagc aacagttccg
    13981 gcaaaaggag caagtactcc aggaacaact caaggccacg gaacgaaaaa ttcaagaact
    14041 gcagagccaa aagcctgaag gaaattccat gatcctgagt gtggaacagc aggaggccat
    14101 gggacaattt cgcaaggagt tgcttcaggt tcgcaaggaa ttaagagctg tgcaacatga
    14161 gcttggcaaa aatattgagc gtgtggagag ttgggtgaaa tttatcaata tcgggcttgt
    14221 gccattgtta attggtatag cgggtgtgtg gatcagttcc tccgggatca ggaaaaagcg
    14281 gccaaaagct taagatgctc agttattgat catcagaaga ccgcatcaga ttattcaaga
    14341 atattaaggg aagcgaatga aagccaaaac actaggaatt ttagccgcca taaccattgt
    14401 ggggattgtt gttgccgttt tcgtgaatca agagccggct tctcagttgc caggtagtgg
    14461 tgaactcttg tttccgtcac tattgtcggt ggtgaataat gtgggcgaag tggtggtgga
    14521 gacgaaggat cagaccgtga cgctggtgcg tgaggagaag acttggcggg tcaaagagaa
    14581 ggcagggtat cgcgctgatg tggagaaggt aaagcagact ctcatcgggt tggcggagtt
    14641 gcggatactg gaacccaaaa cgaaaaatcc agagttatac gatcgattgg gtttacaaga
    14701 caaggagcaa gaagactcgc tttcgacaac cgtgactttg aaaactgcga ataatcctga
    14761 ggcggcagtg gtgattttgg ggaatcaacg tcctgccaag gggaatccac ggatgagtga
    14821 aatctatgtg cggaaaccag gagatcccca aacctggctg acaataggaa atttgccgtt
    14881 ggaaaaagtg tccggagaat ggctggacac ggaaataacg gcactgacga ccaaacgggt
    14941 gcatcgagtg acggtcacgc atcccgacgg ggaaactctt ctcgttttaa aagagaagcc
    15001 ggatgatctt gattttcaat tggattcggt ccctgcaggt tccaaggtgg catctcagtt
    15061 taatgtgaat aatgtggtgg ggactcttgt gcaactcccc ctagaagatg tgaaaaaaga
    15121 agaagaggtg aattttcaga atcacacagg agtaaccgtc gtgttggaaa cctttgacgg
    15181 tctcaggctt catgtccaaa cgaccaaaca ggaagacaaa attttcggaa aattctctgc
    15241 ggaatttgat cagaaactgg ttcaacctgt ggaggacacg cctctctcag aaaaagggga
    15301 aacggtccaa gcccaggatt ctgctcaaga cgaaatggac cttggatcgc ctgaagggaa
    15361 gcaaccagat gaagagcagg ggaagcgtta ctccgaagaa gaatcaatat taaaaaaacc
    15421 ggaagaggtt cagggagaag ttgaagcgtt caatcagcgt gtacaggggt gggcgtatca
    15481 gttgccaacg ttccgggtcg agaacttttc aaaagccaag aaggatcttc ttgccacaat
    15541 tccctaaact agaccattca ggatatagga ggtgatgaaa gattgcttct ctacgtttga
    15601 gacagcttca ggcgttctcg ggcttatccg aaccctttaa gggaggagcg gtcaacgcgt
    15661 ccagtaattc ctgatggacc tgcatcaggc gggaatcctc aagccggtag gcaaaggata
    15721 acaggatcaa gcctcctgcc agcactaaac agcctaaggc ggttatcccg accacccaaa
    15781 aaactccccc aattgtgctc atccctccat caagaataaa aagcacgaca aaggtgagag
    15841 tgcccccaat gacaaaaagt agccataaca gggtgagaat agctgacgac cagggaatac
    15901 gcttggacaa atgaagcagg agttggttgc cggctgaact tacatcaatt gtccccttca
    15961 atggccatgc tgtgcgaaaa ctcagggaaa agagttggta atagggacgg atcacgatga
    16021 cttgtttgtc ttccacccag cgggcaatcc catgaggcaa gggcagtgcg tcagacttgt
    16081 gaaaagcatg ggaaatgggg tgcgctggat gagaatccag agctttctgc ctgtgggcca
    16141 agggacatcc ataccggcag gcccaacttt tcagtgtggt gaattgaaac cagtcaccaa
    16201 tgattaggca tagaaacagc aggatggcaa tgattccggc aaacatcatg ggaaggagaa
    16261 gataccatag tgtcttcttc tgctaaactc ctgtttttgc attgacttct cgaccatccc
    16321 tcctctacta tcgactcgcg gggtggagca gcctggtagc tcgttgggct cataacccaa
    16381 aggtcgaggg ttcaaatccc tcccccgcaa ccacctcggt atcccatcaa agaactcact
    16441 ggtttccacc atgctttcct gcgattttcc tgctttgaaa ctcaagaatg tatttcccct
    16501 gagcctggga gtcgtaatct ctcttgatat gggacccttt taagggttga tttctcttgg
    16561 gttttcgctc tgggaaccga taaatttctg gtgggggtga aagcagctta ctcgagggct
    16621 tgtggaatta ttagccccca attgaatttg aaatggtttg cctcactttt tgacaccaac
    16681 gtacgcgtgg acaggaattg tccatcaagg ctggatttct ttctgctcgg tggatatggc
    16741 taaaccgaaa attttaggga atttcccact agtttcctac ttacgtgcct gtgaaggact
    16801 gtcttgatcc gaatgtgttc gtcagtcaaa tataatttgg atatgaacaa aatttctcaa
    16861 gatgacaaag tccagaagga cagtggtacg ttatcatcca tacagggaat aaccatttct
    16921 attgttgttc ctgtgtacaa cgggggtgag gcctttcgtc aatgcctgat gagcctggtt
    16981 gccttacaac ctcctcccat ggaaattatc gttgttgccg atggtgaatc cgatggatcc
    17041 tggcaagtgg cggaacagtt tggtgctcaa gtcattcgac ttccgaccac ttctggtgga
    17101 ccggcacagc ccagaaacat tggggcactt caggccaaag gcgaatatct tttctttgtc
    17161 gatgccgatg tgtgtgtgca tccggatact ttgcgacagg tagcagaaac gtttcattgt
    17221 aatccggacc tcactgcatt gatcggatcg tacgatgaca cccctgccga aaaaaacttt
    17281 ttatcccaat acaaaaatct ctttcaccat tatgtccatc aacatgctca aaaggaggct
    17341 tccacctttt ggggggcttg cggagccatc cgccgtgata ttttcctgga aataggtggg
    17401 tttgacgaaa aatatcgacg tccttcaatt gaagacattg agttaggata tcgagtgaaa
    17461 aaggctggtc accggattca gttgtgtaaa gatatccatg tcaaacattt gaaacgatgg
    17521 ggtatccgtt caatgctcaa agcagatttt ttttaccgtg ccatcccttg gacagagtta
    17581 atccttcggg atcgcaggtt taccaatgac ttaaacatcc ggcattccga gcgcctgtgc
    17641 gtcgtgttga cgtatgggct cgtgggggca ttgctgggag cgatctggtg gattccattg
    17701 ctgcccgtgg cggggttgat catggtttcc cttgtgatca tcaatgcgcc actttatcgc
    17761 ttttttcaaa aaaaacgtgg gctctggttt gccttgcaga gtattccttg gcattggctc
    17821 tactatctgt atagtggctt agcgtttgcc ataggcctgg cacgacatgt tttttgtggg
    17881 gacaacagga aagagtcaac aaagccagcc cttgaatctg actcctttga cgccataaaa
    17941 aagtcagggg gacaatagtg gcatcccatc ctgtcgtggt gattggtgcc ggtcccgctg
    18001 gtcttacggc ggcctatgag ctgggaaaaa acagttctcc ctccctcatt cttgaggctg
    18061 ggaaacaggt aggtggtata tcgcaaaccg tcaactatcg agatttccgt tttgatattg
    18121 ggggccaccg atttttctca aaagtgccga tggtgactga gttatggaat gaaatcctcg
    18181 gtgacaactt tctccttcgc cctcgtataa gccgaatcca ttacaatcag catttttttg
    18241 actatcccct taaggccacc aatgcgttgg ccggactggg ggtcgttgaa gctctcctgg
    18301 tctgtttcag ttatgccaaa accaaggtat ttccaggtgc ccaagaggat aattttgaac
    18361 aatgggtcat caatcgtttt ggatatcgcc tgtatcagat atttttcaaa acctacacgg
    18421 aaaaagtctg gggaatttcc tgtaccgaga tatccgccga ttgggctgcg caacgaatca
    18481 aaaatctgtc tctgaaagaa gcggttcgca atgccctact tggtcaaagg ggtgggaaga
    18541 agggtgaaat cgtgacctcg ctcattgagc agtttcatta tcctcgccta ggtccgggga
    18601 tgatgtggga acggtgcgag gaattggtgg ctggttttgg atctcagaca ttaaaaggca
    18661 tcaaggtcga gcgtatcaga catcgccatg ggcgggtaga ttgtgtttcc gggcgagcat
    18721 catcagggga acatgtagaa ttcgagggca gcgattttgt gtcgacgatg cctctccgcg
    18781 agttaataga ggccatggac cctcaacctc ctgcgaaggt tttggaggct gcccttggtc
    18841 tgcggtatcg ggattattta accgtggtcc tggtggttaa ctgcgaagac gtattccctg
    18901 ataactggat ctatattcat tcacctgaag tcaaaatggg acgaatacag aattataaaa
    18961 attggagccc gtatatggtg ccggatccct cacggacttc gctgggcctt gagtattttc
    19021 tctgggacaa agacgatgaa tggacatggt cgaacgagcg gttaatcgaa ttcgggacac
    19081 gggaatgcgc gcaactcggg ctcattaatc cccgcgatgt tgaagacgga actgtggttc
    19141 gtatggaaaa ggcctatccg gtttacgatc atcactatca ggatcatgtc cggacgattc
    19201 gagagtatct ggaaacattt tcaaatcttc agaccattgg acgaaatggt ctgcatcgct
    19261 acaacaacca ggatcattcc atggtaacag gagtgtatgc cgcgagaaac atcatgggtg
    19321 aatcgcatca tgatgtatgg gcggtcaata cggaaaagga atatcatgag gagggccgtg
    19381 cggcttcagt taatgccggc gatcgaatgg tgcctatgcg ggttcaggaa aacgtcgatg
    19441 aacttcccat ggaaaacgaa gaagaattaa tcgaggttgt attttccaaa cttgaccctg
    19501 tcgcgctggg tgtggctgtc ggtaccgtga gtggcctcct catcttgatc gggaccgcaa
    19561 ttctggttct caaaggaggg ccggtagtgg ggcccaacct gtctctgctt ggaaattttc
    19621 tatttggatt tcaagtgaca tggagtggtt ctattgtagg tttcctggag ggaggaatcg
    19681 ggggatttgc gataggttac tcaggagcct cactccgcaa ctggagcatg aaggcctttg
    19741 cgaaattcat gctgtggcga gaggaaatga ctcgtcgtcg caatcttcta gattagctac
    19801 cagggtccag gagggaagat atgggaacgg atttcaaaag taatgaagag cttagccggg
    19861 ttgtggccaa aattcaggca ggggtactgg cagtagtttg tgcgctgatg ggtggattag
    19921 gacttttttt catgacagcc tggcttcttc tcgaaggagg gccacaggtt ggctctcatc
    19981 ttcaattgct ttcgaactat tttatcgggt attcagtcac gtggaaaggc agcctggtgg
    20041 ggctttttta tggagcactg accggtggga ttctgggttg gaccatcggg ttcatttata
    20101 acagagtggt gggaatccga ggagggtaaa cggcaatcct cttagttttt cttgattgaa
    20161 tacggatggt gaacgatttc ttaatttgcc aatctgtaaa gggaaaatcg ttacagacta
    20221 tcgtgtaggg aaaaattcag aacacaaaaa ataatgaacc atgaaaatcc tgattatgaa
    20281 ggaagccggt tttggggaaa tcatcaggct aagagtttgc tgaaaacgga ccaggtagga
    20341 tgacgcttta tgtgatcgac caaatttacc tctgttcttg aaaaatctta agtggattgc
    20401 aaagtaaata ttgccaattc gttcgccgtt atacggacta ttcacggata taaccagtcc
    20461 tcctattcaa gtgctcagac attcagggga aatccaccat ctggaaaaga ggtgatggcc
    20521 tgaatggtga gagaaccaga catgtaagtg cccaaccaat tctcgtgccg ccaaatcagg
    20581 cctttcctct atgcacatga tgacaaccca acattctcat tcgggtaaat ggagggttgc
    20641 ttcatcctca ggcggatttg tcagtgtaag gcttgagaac ctacctatta aaatgtcaac
    20701 tcactgttcg gaaagtccga tactatctgg gcgacattgt cgtgggctga tttagcgaga
    20761 gaagaagtat cttggctcag atctaagggc tcttcactcc catttaccaa tcagggaatc
    20821 gacggcgtga taaacgaagg tgcgggctac tgggactcca tagcagacga atggaaaaag
    20881 acacgcccgg atcagctctg gagaaaacat agtgatacgg ttaatcaatc cctacttctc
    20941 cgttggcttc ccgaacagcc atctcctcgg ttgctaaaga ctgatacgtt tgacgaagcg
    21001 gtgggggggg ggcttattca attcctgaag acacgtgctc acaaggtcgt tggaatggat
    21061 ctctcattcc agaatgtgga attggcctgt cccggggata tgagcattcg tggagcatgc
    21121 gcagatgtgc gtcatcttcc tttcggagag gagtcgttcg atgtggtggt gtccaattcc
    21181 accctggacc atttcctaac cctagatgag atcatcgtga gtttgcggga attgcaccgg
    21241 gtgcttcgca aaaacgggca gctaatcctg acattggata atcctgctaa tcccattatt
    21301 gccgcgcgta acattcttcc attcaaatta ttaaaccggt taggtatcct tccctactat
    21361 gttggtgcca cctgcggtcc atggcgctta cagaagttgc tgcgggagat aggattcgag
    21421 gtaagggagg ccacggcagt gctccattgc ccgcgtattt tcgccgtact cgtcgccaag
    21481 ctcttgtgct cctgcgcgaa gggttccgtg caacaacgat ttttgctttg gctggaggca
    21541 tttgaacatc tgtcgcgctg gcccacgcgg tatgtaacag ggaactttat tgctgtcaga
    21601 gcagaaaaaa aataaagcca accgagcagg gtctacaggg attaaatgat ttttctatag
    21661 aatgtttgtg agtcgcgtca tatgcgcgaa cttggttggt caacggggca tttttattgc
    21721 tcactcgtgt tgttgaatca aaacagatgt aagagaagag acgccgcacc aaaacgaatg
    21781 atgttgttag acggatggaa aacttttcgg ttaattcgac aagctctgtc ttccagtcgt
    21841 tggagcctgg tggatctacg tcaggtacag gaggagcgga ttcgggtact cctcaatcat
    21901 gcctaccaca acgttccact ctatcgaagc ctttataagg aggcgggtta tcaccctgag
    21961 gaatttcgtt ctttggatga tataggggaa atccctattc ttcaaaagga tcgtcttaag
    22021 caagcaagtc cggaggaagt tgttgttcag gggattgatc caaaccgatg tagaatcgtt
    22081 gaaacgagcg gttcgacagg aactcccttg cggatctttc ttggatccat agatcagcag
    22141 tggcagcggg tggttgcctg gcgcattcta ttcgaacatg gtttccgctg gactgaccgc
    22201 acgcttgaaa ttcgcatgac ttttggtcgg cagtttttcg tccaacggct tggtatcgct
    22261 ccaaaagatt ggtgttcgat tctggacccg ccagagttct gggcccaacg catggttaag
    22321 acgaggccgg acgtgattgt tgcaggggcg ggtaccttgc atgcattggc ggaaactgtc
    22381 caaacactgg ggttcaagct gcccgcgccc cgacttatca tctccgatag tgaaaccctc
    22441 tcaccggcca cgcgtcaatt ggttcgctgt gcgttgggaa gggatcctgt ggatgtgtac
    22501 gggttggttg aactttcgaa tttcgcgtgg gagtgcgagc gtcattccgg atttcacgta
    22561 agcgctgata gccacattgt ggaggtggct gcggcttcag gggaacctgg tccaatcatc
    22621 accacagcat taggcatgtg gaccatgccg atgattcgct atgaaacagg agatctggcc
    22681 gaggttgaga ccacatcatg tccgtgtggg cgcactcttc cccttctgag gcgaatctat
    22741 ggacgcaggg tggattcagt ggctttgcag gacggcaggc gcatcttctg gccttttttt
    22801 cacgaggtcc tggggcgcta tgacgagtta caccagtgga ggattttgca agataggttg
    22861 aacgaagtcc agctccaaat cgttgtgaca aataacgctc aagatcttct ttcgagaata
    22921 gaatcggatc ttcaagctgt cctgcctggt gaaatccatc tgcaggttga gcgagtggag
    22981 aggatcctgt taagcccggg agagaagacc aggatgatca tctcgaaagt cgggactgca
    23041 attgaggagc agcctgcctc cggaaggcaa gggctctaga tggaaatact gcgaacatgg
    23101 aagtgcctaa tggatctgag gtcgaacgta acacgttctc ccgcacatct cagatcgttg
    23161 caagatcatt tgttccgtgc cgctgcgatc catgcctaca gaaacgtccc gttttaccgg
    23221 cgtgtttggg acgaagcgcg ggtagatgtc caagccattc gaggcattca ggatctggag
    23281 agtattccaa tcattaccgc tcccatggtg cgagaagctg caaaacttgg cgagttgctg
    23341 gcgcgaggag tggacccatc atcctgtacc tatcttgata caagtggctc atcaggtgtg
    23401 gcattacggg tctggaagcg gccgctcgaa gaacgtgtgc gaagagccgt gggactacgg
    23461 atttggttcg aacacggatt tcgttggcgc catcacaccg cccagtttca gataaagccg
    23521 ggtccatcgc acatgctgca gcgcttcgga atttctcgga agacatggat ctcgacggag
    23581 cagtccatcg aggaccagtt ggctcaattg ttggaggcca agcctgatgt ggtggtgggc
    23641 acggcaacgg cacttcgccg cattgcgcga gcgagcgaag cggcccaggt catactcaaa
    23701 cagccacggg tggtgttttg cgcaggagaa ttggtggatg ccgaaacccg tcaattagtc
    23761 acgcgagtct tcaaccgcaa accggtcggg ttgtatggcc agaccgaggt cggatacatc
    23821 gcctggcaat gcgagcagcg tgggtcgttc catgtgaatg cggatacaca ttttgtcgaa
    23881 gtattgcggg atgggatacc ggctcatccg ggcgacttag ggacaatcgt ggtgacagat
    23941 ttgcgtgccc ggacgatgcc actcatacgg tacaatacgg ctgatctggc catagctgca
    24001 tcaggtg
//
LOCUS       DAORAQ010000032        23805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_400_length_23805_cov_64.443874, whole genome shotgun sequence.
ACCESSION   DAORAQ010000032 DAORAQ010000000
VERSION     DAORAQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 23805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23805
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_23805_cov_64.443874"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="mazG"
                     /locus_tag="PKM72_04975"
     CDS             complement(<1..808)
                     /gene="mazG"
                     /locus_tag="PKM72_04975"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNP60170.1"
                     /translation="MNQPSSKPSPSAPPTTCLSDLLAIMARLRGPDGCPWDREQTSQS
                     LKPHLLEEVYEVLEAIDTGKPSALKEELGDLLLQIVFHTQIASEQNHFSFDDVANALA
                     DKLIRRHPHVFQPTTDPTPITTAQDVSRQWDQLKKQEQATQPGPQSVLQGVPKIAPAL
                     QRAFQVQKRASRAGFDWERIEPALEKFQEELNELYTAVAESIPRTQEGTLRPSNLPVQ
                     NTIEEEVGDAFFALVNVSRFLRVNPEEALRRATNKFISRFHFVESQATLEG"
     gene            936..1337
                     /locus_tag="PKM72_04980"
     CDS             936..1337
                     /locus_tag="PKM72_04980"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNP60171.1"
                     /translation="MSEEEQGFVIRDKRGRSEPEASPPPEVKTSAAEPVSSAESPHAP
                     HPPLSFSSFVFSLGTSSLMLMGETLDPQQPPPPVNLPQAKEIVDILSLLEEKTKGNLS
                     SEEASVLGDMLYTLRMKYVSLTSGKGSMVSP"
     gene            1515..3833
                     /locus_tag="PKM72_04985"
     CDS             1515..3833
                     /locus_tag="PKM72_04985"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60172.1"
                     /translation="MNLLSEPLTESRKFPRTGPPSASIESGIPSYSSSSKQKRHHYRP
                     VWIVLVLLLPCLALTLYYARYGLSGPLQPDSILPSPSYALVLFLVYLDSVGLVALTLL
                     LSRNLIRAFFEKRHKLLGKGFRSKLVAAFIGFALIPTVLLALVASGVISEVIDVWFND
                     QIMHVLRDSEEVAHLYQEERESLTADTARAISKEIFREDILKPEHRELLRAIMARMQQ
                     EYHLAGVEVFSPKMETLARMMDPKLSDAVLSLPVGQLVLHVLDTGQPMSSAQEAPVGK
                     LIRAAAPIRGNGQADRVFGVVVVSSYVPEALVMKMDGIAKQFEEYRQILSMKNPIKGG
                     AYLFVAVVTVLILFGATWFGFYVARGITVPIQRLAEGTEAVAKGNLDVKIDVKATDEI
                     GTLVQSFNRMTADLRTSKTSLEEVNMSLVRSNVEIDQRRAYTEAVVETIAAGVLSIDM
                     AGLISTVNPSAERIMGVKGDDLRGRPVHDAFKSFNLQLFQKAYDQMLQEQTESVSMDG
                     QMETPNGRLLTVGLNISRMQNEAGLDLGFVFVFEDRTEFIKAQKTAAWQEVAQRIAHE
                     IKNPLTPIQLSAQRLRKKYAEKSTGFDEIFDQATGVIISEVTRLKGMVDEFSKYARMP
                     LPQMRKTSLHDIIEKVISLYTGAHRDIEFIVELDEGLPSLNCDPEQMHRVLVNLFDNA
                     IQAMNDHGRLWVTTECDWRRHRAVVRVADEGIGIQPDDHKMLFVPYFSKKRTGTGLGL
                     AIVHRIISDHNGTIHAENHHPKGALFTFELPL"
     gene            3937..5310
                     /locus_tag="PKM72_04990"
     CDS             3937..5310
                     /locus_tag="PKM72_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP60173.1"
                     /translation="MAETICLIDDEPAILNTLSSILEDEGYQVLVAKSGLEALKIVRS
                     EVPDLVILDIWMPEMDGLETLKRLRTQFPNILVVMMSGHGSIETAVKSTKLGAYDYLE
                     KPLDLEKVTILVRNALHQRKLEEENLNLRIQVERRFELVGSSPAMERLRELIAMAAPA
                     NSRVLIAGANGTGKELVARAIHLQSPRHNRSFVEINCAAIPETLIESELFGHEKGAFS
                     GASSMKRGKFELADGGTLFLDEIGDMSLATQAKVLRALQEQQFTRVGGTKLINVQVRV
                     IAASNKDLSEEIGKGTFREDLYYRLNVLPIVVPTLKERRDDIPELAQHFLRLHSEEQG
                     IKPKEFSPQGMEALQRHDWPGNIRELRNLIERLLIMVPKTIIDAADVEMFLQGRAPSG
                     VPSLAVGTNYDSLREARNAFEREVISLKLRENNWNVSKTADELKIERSHLHRKIKLLH
                     VELRPEH"
     gene            5285..6700
                     /gene="rlmD"
                     /locus_tag="PKM72_04995"
     CDS             5285..6700
                     /gene="rlmD"
                     /locus_tag="PKM72_04995"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNP60174.1"
                     /translation="MLNSGPNIKPRRTPSPSSTETLSPGSSHPFQIEKLVAGGFSLGR
                     LNGQVVLCAGGIPGESVQVEILSRRKGVSQGKILQVQDPAESRVVPPCPVVGRCGGCQ
                     LQHVQYEEQLSQKRLILEDTLRRIGKIFDVPIFPVIPSPRPYGYRQVLRMGIGEGPDG
                     LFLGFFESGTQQLLPVDTCFLVDDKMRSVIDAVRSSLRSLVIDGMNLESVEIRWSQLE
                     EVGLLVFRGRAVTKEHVERLMNACSEIPNIKGWIYERADAPEHDSRHRLRHEPIVRGA
                     DHVWEAFRGLRLKMGYRSFMQANWDVFQLLGKMVGEWLGHLKGQRILELYAGTGPLGL
                     SLASQGAHVTCVEGNPFAIHDARESLRINEITGCRVRISSVESYLMTVKSGAYDVILL
                     DPPRAGLNPKVVDRLGVLQVPRLFYLSCDAPSLARDIKSLCEKGYTIQRMQPFDMFPQ
                     TAHLETLVELTLSAPEPVDGD"
     gene            6710..7747
                     /gene="purM"
                     /locus_tag="PKM72_05000"
     CDS             6710..7747
                     /gene="purM"
                     /locus_tag="PKM72_05000"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNP60175.1"
                     /translation="MATYREAGVDIQRGDEFVKRIGPMVRSTFRPEVLGDIGGFGGLF
                     RFPSDRYREPILVSGTDGVGTKVKMATSMNRHDSIGIDLVAMCVNDIIVSGAEPLFFL
                     DYLATGHLEVEVGEAIIQGIAEGCRQAGCALIGGETAEMPSCYPQGEYDLAGFAVGVV
                     ERSEMLGPEKIKHGDVLIGVGSSGLHSNGYSLARRVVLELKEWSPETRVPGLRGTVGE
                     ALLKPTLIYVKLVKALLSKSRLHGLAHITGGGITGNVPRVIPESCQAVIDRSSWTPLS
                     LFTVLQEAGAIDQDEMFRVFNMGIGLVVVSPHEETSRILDIIQEQGMTGCVIGEVRER
                     PSSEPKLYYSH"
     gene            7760..8434
                     /gene="purN"
                     /locus_tag="PKM72_05005"
     CDS             7760..8434
                     /gene="purN"
                     /locus_tag="PKM72_05005"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNP60176.1"
                     /translation="MSLALGVLISGRGSNLVAILDAIDRGALDATLKVVASNKLNAAG
                     MERAKERGVPTIYLDPKPFAQETNPREAYDLAMGALLRQHGVEYVALAGYMRIVTPVL
                     IRMYPSKIFNIHPSLLPAFPGLDAQRQALEWGTKVSGCTVHLVTEGVDEGPIIVQAAV
                     PILEGDTVESLSARILEKEHECYPQALQLMAQQRLHLEGRQVHILSNDESIKERMSGS
                     GGTQKK"
     gene            8541..8616
                     /locus_tag="PKM72_05010"
     tRNA            8541..8616
                     /locus_tag="PKM72_05010"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8574..8576,aa:Ala,seq:cgc)
     gene            9063..9572
                     /locus_tag="PKM72_05015"
     CDS             9063..9572
                     /locus_tag="PKM72_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNP60177.1"
                     /translation="MRFSKKSEYALRAMTELTQEFGHGLVQRKSLAERQNIPLGFLEM
                     ILLSLKNAGLIGSRRGAHGGVFLIKSPREVTLGQIIRLLDGPLAPIACVSQTAYQKCR
                     DCPHAATKTCPIQRIMLDVRNAIASVLDHYTLEDFVFPQKGGALLNLKTPPVSGATQK
                     KKKSSDSQF"
     gene            9625..9846
                     /locus_tag="PKM72_05020"
     CDS             9625..9846
                     /locus_tag="PKM72_05020"
                     /inference="COORDINATES: protein motif:HMM:NF021362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HNP60178.1"
                     /translation="METHWRSVVKGISWRAVGTLDTTLLVFIFSGELLLAASVGLTEL
                     VTKIFLYWVHERVWQKIHWGRIYPTTSSP"
     gene            9893..10303
                     /locus_tag="PKM72_05025"
     CDS             9893..10303
                     /locus_tag="PKM72_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNP60179.1"
                     /translation="MARQILMSGALMAFLGVAIGAFGAHGLKPFLSEQMLAAFETGVR
                     YHLIHALAILGAGLSLANVPLRQFRWAGWAFSLGIVLFSGSLYVMSISGVRGLGIITP
                     LGGLCFLVGWGLLARGYWKAFPAENTPFSRPDGT"
     gene            complement(10326..11255)
                     /locus_tag="PKM72_05030"
     CDS             complement(10326..11255)
                     /locus_tag="PKM72_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNP60180.1"
                     /translation="MESFVFEKLTPFLAAKSLVMMEGSLRLLLILVVAYVGIRAVRFG
                     LHRLEIFLLTIRENNDKDRLAREKRVKTLIGLLLTICLTMVWVVAVVMGLDQIGLDIT
                     PIIASAGIVGLAVGFGAQNLVRDVINGFFIILENQVRIGDVAVVNGTGGLVEAISFRT
                     ITLRDQAGTVHIFPNGTVTTLSNMTKDWSAYVMNIGVAYKEDTDRVTEIMREIGKDLQ
                     QDEQLGRKILEPIEIMGVDAFGESEVVIKARIKTIPIEQWGVGREYRRRLKKAFDQNG
                     IEIPFPHRTLYMGETSPPFIVKNTLPQTETPTP"
     gene            11799..13022
                     /locus_tag="PKM72_05035"
     CDS             11799..13022
                     /locus_tag="PKM72_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNP60181.1"
                     /translation="MIGDLMAGITFHHSPKSRGPGGLVSVGLALLAIIGGSSVSEAAV
                     LTKEDFKLYGYVEASYTQNFNFPKNSGANANQLRIFDGDANSFRPNMAQLVFEKEATN
                     GSNAVERAGFRLKLDVGEDVQFTGGRTGDDVDFQEMYVQYVAPLGNGLDIRIGRMNSL
                     VGYEVIESPYNNNYSRSWLFGLGQPFTITGIRVAYAVNEKLSLSMSALNSYTGLTEDN
                     NTAKSVEGLVSYHPTEQLGLNVYGIVGKESSNTRSGSGKRVLVGGYVTFQATDQLGFV
                     VEGYYANQENASAISSGKNARWDGVAGYAIYDFNDQWGLRFRGEIWEDAGGDQSCLGT
                     AGSGGNSNVCTVSAPIAQTLWENTVTLQYKPAPSLITRLEFRYDKSNQNVFLDGKNNS
                     TNNQQTLAVEAIFLF"
     gene            13047..14360
                     /locus_tag="PKM72_05040"
     CDS             13047..14360
                     /locus_tag="PKM72_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNP60182.1"
                     /translation="MNVSIHKASRAIFSGFMSVLVLNGLGSYVWAEEGGASQLNAGDT
                     AWVLISSALVLAMIIPGLALFYGGMVRSKNVLSTMMHSFIAVCLVSVVWVFWGYSFAF
                     APDVGGIIGGLKWLGLAGVGPEPLEGSTIPHLAFMVFQLMFAAITVALISGAIAERMK
                     FSAFALFAVLWVTFVYAPLAHWVWGGGWLAELGDLDFAGGTVVHISSGVAALACALVL
                     GKRHGYGTENMSPHNLPFTVIGASLLWIGWFGFNAGSALAANGVAASAFVATHVATAA
                     GALAWVGVEGLYRGRSTVLGGASGAVAGLVSITPAAGYVGPIAAIVIGFGGGVVCYLG
                     VFLKHKLGYDDALDVLGIHGIGGTWGAVATGLFASMGGGTGLFFGNPGQVVIQFIGVG
                     ATWVFSFVGTFLILKLVDGTIGLRVNREEEVLGCDLTQHRERAYG"
     gene            14471..14809
                     /locus_tag="PKM72_05045"
     CDS             14471..14809
                     /locus_tag="PKM72_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNP60183.1"
                     /translation="MKLIQAIIKPFKLDEVKDALIELGIQGMTVTEVKGFGRQKGHKE
                     TYRGTEYQIEFVPKIKLEIALADERVGEALEAIMRAAKTGSIGDGKIFVSELHSVIRI
                     RTGESGEGAV"
     gene            14854..16167
                     /locus_tag="PKM72_05050"
     CDS             14854..16167
                     /locus_tag="PKM72_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNP60184.1"
                     /translation="MNLFVPRSTMGRLTGLVLWMSLSQPFAVWAGEDGLSKIDTGDTA
                     WLLMSSALVLCMTLPGLALFYGGLVRKKNVLGTIMHTMVILCLISVIWVLCGYSLAFG
                     PDIGGLIGGLEWVGLNGVGLDPSPTYASTVPHQVFMFFQLMFAAITVALITGSVAERM
                     KFKALLLFAVLWSLLIYTPLAHWVWGGGWLAKLGALDFAGGAVVHISSGFGALVCAVM
                     LGKRKGFGADPMPPHDLPMTILGAGLLWFGWFGFNGGSALGANGVAAAAFLATHTAAS
                     AGGLSWMVAEWVHRGKPTVLGVASGVVAGLATVTPAAGFIGPMSALLIGLVAGTVCYV
                     AVVWKMRFGYDDSLDVVGIHGVGGLIGILATGLFASIGAQGLFFGNAGQFGIQVVLAL
                     VTLTFSVIGTYLILKIVDVTVGLRVSPQDEEMGLDLSQHNERAYS"
     gene            16310..17719
                     /gene="glnA"
                     /locus_tag="PKM72_05055"
     CDS             16310..17719
                     /gene="glnA"
                     /locus_tag="PKM72_05055"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNP60185.1"
                     /translation="MTAKEVLDFAKKNKVEMVDVKFVDLMGTWQHFSIPTSELTLDLF
                     KEGSGFDGSSIRGWRMIQNSDMLIVPDPNTACMDPFTEIPTLSIVGDVQDPITRTVYE
                     RDPRGVALRAEQYLKSTKIGDVSFWGPEAEFFIFDHVSFDQNSHSSYYFVDSEEGIWN
                     SGKDGENLGSRPRHKEGYFPVAPVDSQQDIRSEMCREMEKAGIKVEKHHHEVATGGQA
                     EIDLRFDTLVKMADQMMMYKYIVRNVARRHNKTVTFMPKPLYGDNGTGMHTHQSIWKD
                     GKPLFAGKEYAGVSQMCLYYIGGILKHAPALAALTNPITNSYKRLTPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPSCNVYLAFSAMLMAGLDGIQNKIHPGEAM
                     DKNLYDLEPEELGNIPSLPSSLEQALKALEDDHEFLLKGGVFSEDLLEAWIAYKRTNE
                     IDAIRVRPHPYEYAMYFDC"
     gene            complement(17830..20148)
                     /locus_tag="PKM72_05060"
     CDS             complement(17830..20148)
                     /locus_tag="PKM72_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60186.1"
                     /translation="MKKENYTVARPTLQSNIFSVYSGTMTEAERIQSYQTQCPDLRPA
                     LIRDFVQQMDPDYFESFPPAAILEHLALANQLTFERPCAISIRTLPTRQFQLTLVGYD
                     YFSEFATFCGVLSSFGLDIREATIFTSLEKMSPTFPSTASLQGHTSTGSSSQATRGLT
                     RKIVVDVFQVQALEDLKFAEPEQREFQEMVTALLVLLQKNQIRQARRQVNRRLVENLE
                     NMRQKPTELVHPVNITFSNPRAGHETILDISSTDTPAFLYTFANALAMRGIYIVKAKI
                     EVANHRVRNRLYVRGRQGGKIQGKGEQQELRTAATLLKEFTHYLRWAPDPGKALDHFD
                     QFLDLWLEQANTPSHLTKLSQASTLERLAQLFGSSDYLWEDLLRRQHDNLLPMMNQYQ
                     KGPLIRSKSVLTRAIEPLLLKAKTPVHKKQRLNQWKDEELFRIDMRHLLENSPLPDFS
                     QALTNLADVILNQALLHSQQAINPKASLSAPPSMAIFGLGKLGGGELGYASDIEILFV
                     YQTPGKPSRGQTDQKSSDYFERWAQEFLQWIEAKQEGIFHLDTRLRPHGEKGLLANSL
                     QEIQRYYAPEGGAAPFERQALLKLRFLAGNRAVGKAVERHRDQFVYAPDPWDLQTALH
                     LRERQIKELVQPGTTHVKYGAGGLLDVEYTVQYFQLMHGHDHPSIRTPNTLEAIDHLS
                     GEGLFTLEDGAQLKDDYLYFRQLIDGLRIVRGHAQDLVLPPSGSDEMVFLARRLGMLT
                     TNWLQGADDLEHAIHTRMTKIRKQFLQRFPKQ"
     gene            20150..21784
                     /locus_tag="PKM72_05065"
     CDS             20150..21784
                     /locus_tag="PKM72_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60187.1"
                     /translation="MTAFKPHAASEEDPPPARLPDPTPLLLASGLITQDVARILQAYG
                     LQDWKRADQNLQAMAGEPVTRKALATILPAVLRSVARTADPDQAMNEWERYVDSGIQR
                     LQLFQYLAMVPHVVEVMGAAFGNSPAMAQTFIRDPLLVYWIEDDGVLRRRMTRIALEA
                     NVQAALGTVTSFEAKCEALRRMKRREMLRIGIRDLLGIATPVETYTVLSDLASVVIQA
                     VYELVSQELTQRFGDFGGGGSTGKKADGFSVLAMGKLGGWELNYSSDVDLIYVYHAPE
                     GKTEAGRGQTSIARVLYCETLARELTAVLTAPTAEGALFRVDLRLRPEGMVGPLASSL
                     EDALHYYAGRGRTWERLAFLKAKPIAGNLRVGQALIKGLTDFVYGNEEDEGQVFPAIH
                     SLRSQIISKMIRRGEVDRHVKLGIGGIREIEFIVQALQLRWGQAYPKIRDRQTLKALV
                     KLTHIEKLTAQDARLLKESYVFLRNLENKLQMVHEFQTHLLPNKSEEIAKCAVRMGYL
                     KQATVAQAMEAFLSDYRRHTTRVHQLYERILCSSPS"
     gene            21957..>23805
                     /locus_tag="PKM72_05070"
     CDS             21957..>23805
                     /locus_tag="PKM72_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNP60188.1"
                     /translation="MDLNRMTIKLQEALQAASGIAMRRSHQGIDVEHLLLALLEQSGG
                     LAHPILEHTGVSPTAVKNAAEQALQKVPQVQGSGAPPGQVHLSPRLAKVLNQAETEMK
                     ELRDDYLSVEHVILAMVAEGGVFTSMGLKRDKVLAGLQEVRGNQRVTSQDPEGTYQSL
                     EKYGRDLTRLASQGKLDPVIGRDEEIRRVVQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIIKGDVPDGLKNKRLIVLDMGALVAGAKFRGEFEERLKAVLKEIQSSQGQILLFI
                     DELHTVVGAGAAEGSMDAANLLKPMLARGELHLIGATTLDEYRKHIEKDAALERRFQP
                     VVVDQPSVEDTISILRGLKERYEVHHGVRIKDSALVAASRLSNRYIGDRFLPDKAIDL
                     IDEASARLRTEIDSMPAEMDEISRKVLQLEIEREALKKETDAASRTRLTTIEQELKGK
                     QEALKDLKTQWDAEKSSVGKLQTIRQEIEGAKQQIEQAERQYDLNRVAELRYGTLPKL
                     EKSMKEEEERLANQQGTSRLLKEEVDEDDIAEVVSRWTGIPVSRLLQGEVEKLLHLDE
                     WLHKRVIGQDEAVKAVAEAVLRARSGIKDPNRPIGSFLFLGPTGVGKTEL"
BASE COUNT         5548 a         5437 c         6784 g         6036 t
ORIGIN      
        1 ttccttccaa cgtcgcttgt gattcaacga aatgaaatct ggagataaat ttattggtgg
       61 cgcggcgtaa tgcctcttca gggttgactc gaaggaaccg ggagacattt actaacgcaa
      121 agaacgcatc gcctacctct tcttcgatgg tgttttgaac aggcagattt gatggccgaa
      181 gagtcccttc ctgagtccgt ggaatacttt ccgcaacggc cgtatataat tcattcaact
      241 cttcttggaa tttttccaaa gccggctcaa tcctctccca atcaaatcct gcacgagagg
      301 cccgtttttg aacttgaaat gcccgctgga gggctggagc aattttggga actccctgta
      361 aaactgactg aggtccaggt tgggtggctt gttcttgttt tttaagttga tcccattgcc
      421 ggctcacatc ttgtgctgtt gtgataggag taggatcggt ggtcggttgg aaaacgtggg
      481 ggtggcgtcg aatgagttta tcggctaaag cgttagccac atcatcaaat gaaaaatggt
      541 tctgttctga agcaatttgt gtatgaaaca caatctgaag taagaggtct cctaattctt
      601 cctttagagc ggaaggcttc ccggtatcta tggcctccag cacctcatat acttcctcaa
      661 ggagatgggg ctttaaggac tggctggttt gttcacggtc ccaagggcaa ccgtccggtc
      721 cacgcaatct tgccatgatt gccagtaaat ctgacaaaca tgttgtcgga ggagcggagg
      781 gggaaggctt tgaggatggt tgattcatgt agttttcaaa aaatatgagt atggcgcgag
      841 ggcattatac ccgttttctt tctcagtgta agtccttgat tcttgataga gcccattggt
      901 tagtgctact gttttctcac tagaggaggt gttctatgag cgaagaagaa caaggtttcg
      961 taattcggga taaacgaggg cgatcggagc cggaggcttc cccacccccg gaggtaaaga
     1021 cttcagcggc ggaacccgtt tcttcggcgg aatcacccca tgcacctcat cctccattgt
     1081 cattttcatc gtttgtcttt tctctcggaa cctcttctct catgttgatg ggagagacct
     1141 tggatcctca acagccccca cctcccgtga atcttcctca agccaaggaa attgttgata
     1201 tcttatcctt actggaagag aagaccaaag gaaatctgtc gtcggaagaa gcgtccgtct
     1261 taggagatat gctctatacc ctgcggatga aatatgtcag tttaacgtca ggtaagggca
     1321 gtatggtttc cccctaattg ctggcgaacc cttttccttc ccacaaactt ccgggcgagt
     1381 gcttcttctt tcggctttag gcagacaggc agatcgacag tgggtatgtg gtcgcacctt
     1441 gcgaagatac cgttcttgat caataatgat catagttgtc tagcctttgg gtgttattct
     1501 ccgactgaca tgccgtgaac cttctttcag aaccgctcac tgaatcccgg aaatttcctc
     1561 gaacggggcc accgtctgcg tccatcgagt cgggtatccc ctcttacagt tcttcatcaa
     1621 aacagaaacg gcatcattat agaccggtct ggattgtgct ggtcttactc ctaccctgtc
     1681 tggccctcac tctctattac gcgcggtatg gcttatcagg ccctctgcaa ccggattcga
     1741 ttcttccgag tcccagctat gccctggtgc tatttctggt gtacctggat agcgtggggt
     1801 tggtggcgct caccctcctc ttgtctcgaa atttaattcg agcttttttc gagaagcggc
     1861 ataagctgct ggggaagggg ttccggtcaa agttggtcgc ggcctttatc ggctttgccc
     1921 tgatccccac cgtgctactg gccttggtgg cgagtggggt catcagtgaa gtcatcgacg
     1981 tgtggtttaa tgatcagatc atgcatgtct tacgggattc ggaagaagtc gcgcatctct
     2041 atcaggagga gcgtgagtca ttaaccgcgg atacggcgcg cgccatcagt aaagaaattt
     2101 tccgggaaga tattttaaaa ccggaacatc gggaattatt gcgagccatc atggcccgca
     2161 tgcaacagga atatcatcta gcgggggtgg aggtcttttc gccaaaaatg gaaaccctgg
     2221 cccgcatgat ggatcccaaa ttgtctgatg cggtgttgag cctccctgtg gggcaattgg
     2281 tgctccatgt attagatacg ggtcagccaa tgtcatcagc ccaggaagct ccggtgggga
     2341 aactgattcg ggcggcagcg ccgattcgag ggaatggtca agcggaccgg gtgtttggcg
     2401 tcgtggtggt gtcgtcctat gtgccggaag ctttggtgat gaaaatggat gggattgcca
     2461 aacaatttga ggaatatcgc cagattctat ccatgaaaaa tccgatcaag gggggagcct
     2521 acttatttgt ggctgtggtg actgtcctga ttttatttgg cgccacctgg tttgggtttt
     2581 atgttgcccg agggattacc gtccccattc aacgattggc tgaagggacc gaggcggttg
     2641 ccaagggtaa tttggatgtg aaaatcgacg tcaaggcgac ggatgaaatc gggacgttgg
     2701 tccaatcgtt taatcgcatg acggctgatt tgcgtaccag taaaaccagt ttggaagaag
     2761 tgaatatgtc actcgtacga tccaatgtgg agatcgacca acggcgggcc tacaccgaag
     2821 cggtggtgga aaccattgct gccggagtgc tatcgatcga tatggccggt ctgatctcta
     2881 ccgttaaccc ctctgcagag cggattatgg gagtgaaggg agacgatttg cgcggacgac
     2941 cggtccatga cgcgttcaag tccttcaacc tgcaattgtt tcagaaagcc tatgaccaga
     3001 tgctccagga acagacggaa agtgtgtcca tggatggcca gatggaaaca cccaacgggc
     3061 gcctcttgac cgtaggcctt aatatttcca gaatgcaaaa cgaggccggc ttggacctgg
     3121 gctttgtgtt tgtctttgaa gatcggacgg aattcattaa ggcgcagaaa acagcggcct
     3181 ggcaggaagt ggcgcaacgc attgctcacg aaattaagaa tccgttaacc ccgattcaat
     3241 tgtctgccca acgtttgcga aaaaaatacg cagaaaaatc tacagggttt gatgaaattt
     3301 tcgatcaagc taccggggtc atcattagcg aagttacacg gttaaagggg atggtcgatg
     3361 agttttcaaa atatgccaga atgccgcttc ctcagatgcg gaagacttca ctccatgaca
     3421 ttattgagaa agtgatctcc ctctatacgg gggctcatcg ggatattgaa tttattgtcg
     3481 aattagatga aggcctaccc tccctgaatt gcgatccgga gcagatgcac cgggtgctgg
     3541 tcaatttatt tgataatgcg attcaggcca tgaatgatca cggccgtctt tgggtgacga
     3601 cagagtgcga ttggcgtcgg catcgggccg tagtcagagt ggcagatgaa gggattggta
     3661 ttcagccgga tgatcacaaa atgttgttcg tgccgtattt ttcaaaaaag cgaacgggta
     3721 cagggttggg attagccatt gtgcatcgta taatctccga tcacaatggg accattcatg
     3781 cggagaatca tcatccaaaa ggtgcgttat ttacattcga attgccgttg taggagaggg
     3841 gagcgtcagt ggctggacgg ttcttttctc cggaagggtt gtctcgcgtg acacacgtga
     3901 gagaccattc attccacacc tgatggaggg cgtattgtgg ccgagaccat ttgcctgatt
     3961 gatgacgaac cagccattct caatacctta agcagcattc ttgaggatga aggctatcaa
     4021 gtcctggtgg cgaagagcgg cctggaggcg ctcaagattg tccgaagtga ggtcccggac
     4081 ctggtcattt tggatatctg gatgccggag atggatgggc tggagacgtt gaagcgattg
     4141 cgaacccagt ttcccaacat cttggttgtg atgatgtccg ggcatggatc cattgaaacc
     4201 gctgttaaat ccacaaaact gggcgcttat gattatttag agaaaccctt agatttagaa
     4261 aaagtcacga ttttggtgcg aaatgccttg caccaacgca agctggaaga ggaaaacctg
     4321 aatctcagga ttcaggtgga gcggcgattt gaattggtgg gttcttcccc tgctatggag
     4381 cggcttcgcg aactgattgc gatggcggct ccagccaata gtcgtgtgtt gattgccggg
     4441 gccaatggga cagggaagga acttgtcgcc cgcgccattc atttgcagag cccacggcat
     4501 aatcgttcct ttgtggaaat caattgtgcc gcgattccgg aaacgttgat cgagagtgaa
     4561 ttattcggtc atgaaaaagg ggctttcagt ggcgccagct caatgaaacg tggcaagttt
     4621 gagctggctg acgggggaac tctgttttta gatgaaatcg gggacatgag tctggccacg
     4681 caagccaaag tgttgcgtgc ccttcaagag caacaattta cacgggttgg cggaacaaaa
     4741 cttatcaatg tccaggtccg ggtcattgct gcatccaata aagatttgtc ggaagaaatt
     4801 ggaaaaggga ccttccgtga agatctctat taccggttaa atgttctgcc gattgtggtt
     4861 ccgaccctga aggagcgtcg tgatgatatc cctgaactcg cgcagcattt tttgcgactt
     4921 cattcggaag agcagggtat aaaacctaaa gagttcagtc cgcagggcat ggaggcgttg
     4981 caacggcatg attggcctgg aaatattcgg gaactccgga atttgattga gcgtttactc
     5041 atcatggtgc cgaagacaat cattgatgcg gcggatgttg agatgtttct ccagggccgg
     5101 gcgcctagcg gggtgccctc tttggccgtg gggaccaact atgactcact ccgggaagcc
     5161 cgtaatgcct ttgagcgtga agtcatttct ctaaaactca gggagaataa ttggaatgtc
     5221 tccaaaaccg ctgatgaatt gaaaatcgag cgtagtcatt tacacagaaa gattaagcta
     5281 cttcatgttg aactccggcc cgaacattaa gcctcgcagg actccttctc cctcttctac
     5341 tgaaacgttg tctcccggat cttcccatcc tttccagatt gaaaaactgg tggcaggcgg
     5401 attcagtctc ggacgactga atggccaggt ggtgttgtgt gccggtggca ttcccggtga
     5461 atccgtacag gtggagatac tcagtcggcg caaaggcgtg agccagggga agatactcca
     5521 ggtccaggat ccggctgaga gccgtgtagt tccaccctgt ccggtcgtcg ggcgatgtgg
     5581 gggatgccag ctccagcatg tgcaatacga agaacaactg tctcaaaaac gattgattct
     5641 ggaagatacc cttcgtcgga ttggaaaaat tttcgatgta ccgattttcc cagtgatccc
     5701 gtctccgagg ccctatggct atcggcaggt cctgcgcatg ggaatcgggg agggaccgga
     5761 cggattattc ctgggatttt ttgaatcagg gactcaacag ctgctaccgg ttgacacatg
     5821 ttttttggtg gacgataaaa tgcgatcggt tattgatgct gtccggtcca gccttcgctc
     5881 cttggttatt gatgggatga atttggagag tgtggaaatc cgatggtccc aactggaaga
     5941 ggtggggctg ttggtattcc gcggtcgagc cgtgaccaaa gaacatgtcg agcgtctgat
     6001 gaatgcctgt tctgagattc ccaacatcaa gggatggatt tatgaacgcg cggatgcacc
     6061 agaacatgat tcgaggcacc gtttgcggca tgagcccatt gtgcgaggag cggatcatgt
     6121 ctgggaagcc ttccgaggat tgcgcctgaa aatggggtat cgatcattta tgcaggccaa
     6181 ctgggacgtc tttcaattgt tggggaagat ggtaggggag tggctgggtc atctgaaagg
     6241 gcaacgaatt ttggagttgt atgctggaac cggcccttta ggtctcagcc tcgcgagcca
     6301 gggtgctcac gtgacctgtg tggaggggaa tccgtttgcc atccacgatg cccgtgagtc
     6361 cctgcgtatt aatgagatta cagggtgtcg tgtgcgaatc agttcggtcg aatcctatct
     6421 catgactgtc aaatccggcg cctacgatgt tatcctcctg gatcctccgc gtgcggggct
     6481 caatccaaaa gttgtggatc gcttgggagt cttgcaagtc cccagactgt tctatctttc
     6541 ttgcgacgcc ccgtcattgg cccgggatat taaatcgtta tgtgaaaagg gctataccat
     6601 tcagcgcatg caaccctttg atatgtttcc ccagacggca catctcgaaa cattagttga
     6661 attgacgctt tcagcacctg aaccggtcga cggagattaa ggatacgcca tggccaccta
     6721 tcgagaagcg ggagtagata ttcaacgagg cgatgaattt gtcaaacgaa ttggtcctat
     6781 ggttcggtcc accttccgtc ctgaagtctt gggagacatc ggagggttcg ggggcctctt
     6841 tcgatttcct tccgaccgct atcgtgagcc gattctggtg tccgggacgg atggcgtcgg
     6901 gaccaaggtc aagatggcca cctcgatgaa tcgacatgat tcgatcggaa ttgatttggt
     6961 cgcgatgtgc gtcaacgaca tcattgtgag cggagcggag ccgctctttt tcctggatta
     7021 tctcgcgacg ggtcatctag aagtggaagt cggagaagcg attattcagg gaattgccga
     7081 gggatgccgc caagccggtt gtgccttaat cggtggagaa accgcagaga tgccgtcatg
     7141 ttacccgcag ggtgaatacg atctggccgg ttttgcggta ggggtggtgg aacggtccga
     7201 gatgttgggc ccggaaaaaa ttaaacacgg ggatgtgttg attggagtcg gatcaagtgg
     7261 ccttcatagt aatggctatt ctttggcgcg tagagtggtg cttgagctga aggagtggtc
     7321 tcctgagacc cgggtgcccg gtctgcgtgg cacggtcgga gaggcattac tcaagccgac
     7381 tcttatttac gtgaaactag tcaaagcctt attgtcgaag agccgattgc atggtctggc
     7441 gcatatcacc ggtggtggaa ttaccgggaa tgtgccacgc gtcattccgg agtcctgcca
     7501 agcggtcatc gatcgaagct cgtggacgcc actctcgttg ttcaccgttc ttcaagaagc
     7561 aggagccatt gatcaagacg aaatgtttcg cgtgtttaac atgggcattg gtctggtcgt
     7621 ggtgagcccc catgaagaaa ccagccggat attggacata attcaggaac aagggatgac
     7681 aggctgtgtc attggagagg tccgggagcg cccgtcttct gagccgaagc tttattattc
     7741 gcattaaaag gttgaaactg tgtcgcttgc acttggagtc ttaatctccg gccggggatc
     7801 aaaccttgtc gcaattctag atgccattga tcggggcgca ttggatgcga ctctcaaggt
     7861 cgtagccagt aacaagctca atgccgcggg gatggagcgg gcgaaagagc gaggcgttcc
     7921 cacgatctat ctagatccaa agccttttgc ccaggagacc aaccctcgcg aagcctatga
     7981 tctggccatg ggtgcccttc ttcggcaaca tggagtggaa tatgtggccc tggcgggata
     8041 tatgcggatt gtgacccccg tcttaattcg gatgtaccca tcgaagattt ttaatattca
     8101 tccctcactc ctgccggcgt ttccaggact cgatgcgcag cgccaagcct tggagtgggg
     8161 aacaaaagtg agcggttgca cggtgcattt ggtgacggaa ggggtcgacg aagggccgat
     8221 tattgtgcaa gccgccgtgc ccattcttga aggggatacg gtggaatcct tatccgctcg
     8281 tatattggag aaagagcatg aatgttatcc tcaagctctt caattgatgg cacaacaacg
     8341 attacatctg gaagggcgtc aggttcacat tctttccaat gacgaatcga ttaaagagag
     8401 gatgagtgga tcgggaggca cacaaaaaaa atagatcagc gatggcaaca cgactaatag
     8461 tcagttataa tccttcgata tttttcaatt cataagactt tcttttttcc aacgctgcat
     8521 atagcaggaa tctcaagtgt ggggggctag ctcagttggg agagcactac gttcgcaacg
     8581 taggggtcgc gggttcaact cccgtgccct ccaccatttt tatttgcgtc ttgccccccg
     8641 gcaactcaat tcgcgggttc cggttcgaga attctcttct cacttccacc aggttttaaa
     8701 aatttcctca tttctattcg aaaccagata gatccatttc tcacctgacg tcctgttgtg
     8761 tctttccaat cgcgacggga ttcccatcaa ttttcgggca ctggtcttct gaaagctaaa
     8821 acctccttgt cgcaaatttt cttttggtaa caatgcgcca ggaggatgct catttacctg
     8881 agggcattct ttaacttttc ttcaacggtg attgtgtgtg ttggcaattc tttcgtcaac
     8941 aaactgccaa tggtggacgg ctttttttgg taacctgtgt ttggaaattc aggttcggga
     9001 tagaaaatga aaagggcatt atgctttaat ctgcataata ttagttaact ttatgtagta
     9061 ttatgcgatt ttctaaaaaa agcgagtatg ccttgcgggc catgaccgag ctgactcaag
     9121 agttcgggca tggactcgtg caacggaaat ccctggctga gcggcagaat attccattgg
     9181 gttttctgga aatgattttg ttgtcgttga aaaacgccgg gctcatcggg agtcgtcgtg
     9241 gtgcccatgg cggggtgttt ttaattaagt cgccacggga agtgacattg gggcagatta
     9301 ttcgccttct ggatggtccg ctggcaccaa ttgcctgtgt gagtcagacg gcctatcaga
     9361 aatgccggga ttgtccccat gcggcaacga agacatgtcc aattcagcgc atcatgctgg
     9421 atgttcggaa cgcaatcgcg tcggtccttg atcattacac tttggaagat tttgtatttc
     9481 cccagaaggg aggggcacta ctcaatctga aaacaccacc agtgtcaggt gcaacacaga
     9541 agaaaaagaa gtcgtcggat agtcaatttt agccagtctt tttttgctgt cgtcatgcgg
     9601 caaggtcaac gataagagga acctatggaa actcattggc gtagtgtggt gaaaggcata
     9661 agttggcggg cagtgggtac cttggatacc actctcctgg tgtttatttt ttcgggtgaa
     9721 ctccttcttg ccgcttccgt tggcctgacg gaattggtca cgaaaatatt tttatattgg
     9781 gtccatgagc gagtctggca aaaaattcac tgggggcgga tatatccgac aaccagttct
     9841 ccatagtttc tccatgaatg atattttctt ccatcacaaa aggataaatg atatggcacg
     9901 gcaaatactg atgagtgggg cgctgatggc tttccttggt gtcgcaattg gcgcttttgg
     9961 cgcgcatgga ctcaagccat ttctttcgga acagatgttg gcggcctttg aaactggtgt
    10021 ccggtatcat ttgatccatg ccttggccat tcttggtgct ggattgagtc tggcgaatgt
    10081 tccccttcgc cagttcagat gggcaggttg ggccttttcc ctgggcatcg tgttgttttc
    10141 aggaagcctg tatgtcatgt ccatttccgg tgtgcgcgga ttggggataa tcacgccttt
    10201 gggaggcctg tgttttctgg tgggatgggg cttgcttgcg cgagggtatt ggaaagcctt
    10261 tcccgctgag aacactccat tctctcggcc ggatggtacc tagtcgagac ctcctcttgc
    10321 tctgattagg gcgttggtgt ttctgtctga ggcaatgtgt ttttgacgat aaatggcggg
    10381 ctggtctctc ccatataaag cgttcgatgg ggaaaaggaa tttcaatgcc attctggtca
    10441 aaggcttttt tcagacgtcg ccggtattct cgcccgactc cccattgctc gatgggaatg
    10501 gttttgattc gagctttgat gacgacttcc gattccccaa aagcatctac gcccattatt
    10561 tcaatgggtt ccaggatttt cctgcccaat tgctcatctt gttgcaggtc ttttccgatc
    10621 tcgcgcatga tttctgtcac gcggtcagtg tcctctttat aagccacgcc gatattcatg
    10681 acataggctg accaatcttt cgtcatattg gaaagggtcg tgacggtgcc attcgggaaa
    10741 atgtggaccg ttcccgcctg gtcgcgtagg gtaatggttc taaagctgat ggcttcgact
    10801 aagccaccgg ttccattgac cacggccaca tccccgatcc gaacctggtt ttctaaaata
    10861 ataaaaaatc cgttaatgac atctcgaacc agattttggg ctccaaagcc gacggccagt
    10921 cccacgatgc ctgcactggc gataatgggt gtgatatcca accctatttg atccaagccc
    10981 atcacaacgg caacgaccca aaccattgta agacagatgg tcagaagcaa gccgatgaga
    11041 gttttaaccc gtttttcacg ggccaggcgg tccttatcat tattttccct aattgtgagt
    11101 aaaaagattt cgagtctgtg caggccaaaa cgaacggcac gtatcccgac ataggcaacc
    11161 acgaggataa ggagaaggcg caagctgcct tccatcatga cgagcgattt ggctgcgaga
    11221 aaaggagtga gtttttcaaa aacaaaagat tccatccatg acctccttaa aagagaaaga
    11281 gaatagttaa ttggtgaagg actgtttcaa ctcggcccaa tcgagagtct gagccagaga
    11341 agaatgccta ttctttcaag ggaccggagc attggcaagc cggaagggac tgcattgcta
    11401 tgtatctaca atccgggctg aagaatttga gaatgaagtc aattgtaggg gggttgccga
    11461 gcgacttttt tttcacggag accttaggcc acatatttta gggctctccc ttgacacatg
    11521 ggatcttggg tggtaaaaaa ctcaaaatag gtcgtcaagg acaatgaagt ccaatggtcg
    11581 gcctccatac aatagaataa cccggacaat gaagtcctca gccaaaaatt tggttgggga
    11641 tttttttttg cctttttagc ccatgacctc tctttggagt tggagaaacc ccaaaaattt
    11701 tggtcaatga agcgtggctg atcatgaccg aggcaaggtg gcgtggaaaa ttttggtcgt
    11761 gttttggcga gtgcgacctc aaaaacatgg agggctacat gattggagac ctaatggctg
    11821 gaatcacctt tcaccactca cctaagagtc gaggacctgg agggctggtc agcgtcggtc
    11881 ttgcgctgct tgcgattatt ggcggatcga gcgtgagtga ggcagcggtg ctcaccaagg
    11941 aggatttcaa actctatggg tatgtggagg cgtcctacac tcaaaatttc aactttccca
    12001 aaaattccgg ggccaatgcg aatcagttgc gcatcttcga tggggacgcc aattccttca
    12061 ggcccaatat ggcccaattg gtctttgaga aggaagcgac gaacgggagc aatgccgtcg
    12121 agcgtgccgg cttccgcctc aaattagatg tgggtgagga tgtgcaattt acaggcgggc
    12181 gaaccgggga tgatgtggat ttccaggaaa tgtatgtcca gtatgtcgcc ccgctgggca
    12241 acgggttgga tattcggatt ggacgaatga attccctggt gggatatgaa gtcattgaga
    12301 gtccctacaa taataattat tcccgatcct ggttgtttgg attgggtcag cccttcacga
    12361 tcaccggcat tcgtgtggcc tatgcggtca acgagaagct ttccttgtca atgagtgccc
    12421 tgaattccta taccgggttg acggaagata ataatacggc gaaatctgtg gaagggttgg
    12481 tctcctatca ccccacggaa caacttggat tgaatgtgta tggcattgtg ggaaaagaga
    12541 gttcgaatac gagatccggg tcagggaaac gggttctggt cggggggtat gtgaccttcc
    12601 aggctaccga tcaattaggc tttgtggtgg aggggtatta tgccaatcag gaaaatgcaa
    12661 gtgcgatcag ctcgggcaaa aatgcccgtt gggatggggt agccgggtat gccatttatg
    12721 atttcaatga ccaatggggc ctccggtttc gtggagaaat ttgggaggat gccggcggcg
    12781 atcagagttg cttggggacg gccggatccg ggggcaattc gaatgtgtgt accgtttctg
    12841 ccccgatagc ccaaacgctg tgggagaaca cggtgacgct tcagtataag ccggctccct
    12901 cgctgatcac gcgattggaa tttcgctatg acaaatccaa tcaaaatgtc tttttagacg
    12961 gaaaaaataa ctcaactaat aaccaacaaa cactggcagt tgaagccatt ttcctctttt
    13021 aagaagaggt agaaggagca aagacgatga acgtctctat tcataaagcc agtagagcca
    13081 tattttccgg atttatgtcc gtcctggtat tgaacggttt gggttcatac gtgtgggcgg
    13141 aggagggagg cgcgtcgcaa ctcaatgccg gcgacacggc ttgggtgttg atatcctcgg
    13201 cgttggtgtt ggccatgatc attcccggat tagcgttgtt ttatggcggg atggttcgta
    13261 gtaaaaatgt attaagtacg atgatgcata gctttatagc ggtctgtctc gtcagtgtgg
    13321 tgtgggtatt ttggggctat agttttgcgt ttgctcccga tgtgggaggg atcattgggg
    13381 gactgaagtg gttgggatta gccggggtgg gacccgaacc gcttgaaggt tcgacgattc
    13441 cccatttagc ctttatggtg tttcagctca tgtttgcggc gattacggtc gcgctcatta
    13501 gcggagccat tgccgagcga atgaaattta gcgcctttgc cttatttgcg gtgttatggg
    13561 tcacgttcgt ttatgccccc ctggctcatt gggtatgggg gggaggctgg ttggctgagt
    13621 tgggggactt ggattttgcc ggcgggacgg tcgtccatat tagttccggc gtggctgcgt
    13681 tagcctgcgc ccttgtctta ggaaaacgcc acggatatgg taccgagaat atgagccctc
    13741 ataacctccc gtttacggtc attggggcca gtttactgtg gatcgggtgg ttcggtttta
    13801 acgcgggaag cgccttggcg gctaatgggg ttgcggccag cgcctttgtg gccactcatg
    13861 tggcgacagc cgcaggagcg ttagcctggg tcggagtgga gggactctat cggggaagat
    13921 caacggtgtt gggcggggct agcggcgccg tcgcggggct ggtgtctatt acgccagccg
    13981 ccggctatgt gggtcccatc gcggcgattg tgattggttt tggaggaggc gtggtgtgtt
    14041 atctcggtgt atttttaaag cataaactgg gctatgatga tgccttagat gtgcttggca
    14101 tccatggaat aggaggaacc tggggggcag tggctaccgg gttattcgcc agcatgggtg
    14161 gaggcaccgg gctctttttc gggaatcccg ggcaggtcgt cattcagttc attggagtcg
    14221 gtgcaacctg ggtgttctca tttgtcggca cctttcttat tctcaagctc gttgacggca
    14281 cgatcgggtt acgcgtcaat cgggaagaag aggtgcttgg atgtgatctg acccaacacc
    14341 gggaacgggc ctatgggtaa tatcaaaaag aggtgaaggt ttcgagagca agtgacggtc
    14401 agatggtccg tcgccaccac gaatgaaaag atttttcagc gtgtcttaat ccaatggaag
    14461 gatggttctt atgaagctga ttcaagccat tattaaacct tttaaattag atgaagtgaa
    14521 agatgccttg atcgagttgg gtattcaagg catgactgtc accgaagtca agggatttgg
    14581 tcgtcaaaaa ggacataaag agacctaccg gggcactgaa tatcagattg aattcgtccc
    14641 aaagattaaa ctggaaatcg ccttggccga tgagcgggtg ggggaagcgc tggaagccat
    14701 tatgcgtgct gccaaaaccg gcagtattgg ggatggaaaa attttcgtgt ccgagctgca
    14761 tagtgtcatc cgtattcgta cgggggaatc aggcgaagga gcggtctgac cattgccacc
    14821 ctatcacacc acgtgtttac tcgaggaggg gaaatgaatc tgttcgttcc acgctcaacg
    14881 atgggaaggt tgaccggatt ggtcttatgg atgagtctga gtcagccctt tgctgtttgg
    14941 gcaggtgaag acggtttgtc aaaaattgat acgggggata cggcctggct tttaatgtct
    15001 tctgcgctgg tgttatgtat gacgcttccc ggactggccc tattttacgg cgggctggtt
    15061 cgcaaaaaaa atgtgctcgg aaccattatg cataccatgg tgattctttg tttaatcagc
    15121 gtgatttggg tgttgtgcgg atacagttta gccttcggtc ctgatattgg agggttaatc
    15181 gggggactgg aatgggtggg gctgaatggt gtgggattgg atccgagtcc gacctatgcg
    15241 tcaacggtgc cgcatcaagt ctttatgttc tttcaactca tgtttgcggc cattacggtc
    15301 gcactcatta cgggcagtgt agccgagcga atgaaattta aagccttatt gttgtttgcc
    15361 gtcttatggt ctctcctgat ttatacccca ttggcacatt gggtgtgggg tggagggtgg
    15421 ttagccaagc ttggagcctt ggattttgcc ggcggtgcag tggttcacat ctcttcaggg
    15481 ttcggggcgt tggtctgcgc ggtgatgctt gggaaacgca aggggtttgg ggcagatccg
    15541 atgcccccgc atgacttgcc gatgaccatt ctgggagcag gacttctctg gttcgggtgg
    15601 ttcggcttta acggggggag tgcgctcgga gccaacggtg tggctgcggc cgcgtttctc
    15661 gccacgcaca cggccgcgtc ggcaggggga ctcagctgga tggtggctga atgggtccat
    15721 aggggtaagc cgaccgtgtt aggggtcgcc agtggggtgg tcgcgggttt ggccacggtg
    15781 actccggctg caggatttat cgggccgatg tcagccctct tgattgggtt ggttgccggc
    15841 accgtctgtt atgttgcggt cgtgtggaaa atgcggtttg gctatgatga ttccctggat
    15901 gttgtcggta ttcatggcgt gggcggattg ataggcatcc tggctacagg gttgtttgcc
    15961 tcaatcggag cccagggttt gtttttcggg aacgccggac agttcggtat acaagtcgta
    16021 ttggctttgg taaccttgac cttttctgtc attggaacct atctcattct taaaatcgtg
    16081 gatgtgacag tcggactgag agtctccccc caggacgagg aaatgggatt ggatcttagt
    16141 caacataatg agcgagccta ttcctaaagc aggagggatg tggatgagca tgattcggtg
    16201 gaacggtcat tgtctctgat ccacttctga tgagtgcctg gtagtggttg cggcggtgag
    16261 agcgtgggat tacttttata aacagatgtg cttgcgaagg aggtattgaa tgaccgcgaa
    16321 agaagtgttg gattttgcga aaaagaacaa ggtggaaatg gtcgatgtca agtttgtcga
    16381 tctcatgggt acctggcagc atttttcgat tcccacctca gaattgacgt tggatttatt
    16441 taaagagggc tcaggatttg atggatcctc tatccgtggc tggcgcatga tccaaaatag
    16501 cgacatgctg atcgtgcctg atccgaacac cgcctgcatg gatccgttca cggaaatccc
    16561 gacgttaagc atcgtggggg acgtgcaaga tccgattacc cgaaccgttt atgagcggga
    16621 tcctcgtgga gtagcccttc gcgcggaaca atatctcaag agcaccaaaa tcggggatgt
    16681 ttccttttgg gggcctgaag ccgaattttt tatttttgac catgtgtcgt tcgatcaaaa
    16741 cagtcactcc agttattact tcgtcgattc ggaagagggc atctggaatt ccgggaaaga
    16801 cggagagaac ctcgggtctc gaccgcgcca taaggaggga tatttccccg tggcccctgt
    16861 cgactcgcaa caggatatcc ggtcggagat gtgccgggaa atggaaaaag ccgggatcaa
    16921 agtggagaaa catcatcatg aagtggccac gggcgggcaa gctgaaatcg accttcgatt
    16981 cgataccctg gtcaagatgg ccgatcagat gatgatgtac aaatatattg tgcgaaatgt
    17041 cgcgcgtcgg cataacaaaa ccgtcacttt tatgcccaag ccgctctacg gggataatgg
    17101 caccgggatg catacccatc agagtatctg gaaagacggg aaaccattgt ttgccgggaa
    17161 ggaatatgcg ggggtctcgc agatgtgcct gtattatatc ggcggcattc tcaagcatgc
    17221 gccggcgtta gccgcgctca ccaacccgat tacgaactcg tataaacggc tcaccccggg
    17281 ctttgaagcg ccggtgttgc tggcctactc cagtcggaac cgatcggcgg gcatccggat
    17341 acccatgtat tcgccaagtc ccaaggccaa acgcattgag gtccggttcc ctgatccctc
    17401 gtgcaatgtg tatttggcct tcagtgccat gctcatggcc gggttagacg ggattcaaaa
    17461 taaaattcat ccgggcgaag ccatggataa gaatctctat gacttagagc cggaggaatt
    17521 agggaatatt ccctcattgc cgtccagtct ggaacaggca ttaaaggctc ttgaggatga
    17581 ccatgaattt ctcttgaaag gcggagtgtt ttccgaggac ctgcttgaag cctggattgc
    17641 ctataaacgg accaatgaaa tcgatgccat tcgagtcaga ccacacccgt atgaatatgc
    17701 tatgtacttt gattgctaag cgctgaaaaa tggccttccc cacattcttg tggggaaggc
    17761 atacggttta tactcacgcc cgggcaagtc aaatgaagga gataactatt tccttttgat
    17821 gcccgggcgt tactgtttgg gaaaccgttg aaggaattgt ttccggattt tcgtcattct
    17881 ggtgtgaatg gcatgctcca gatcgtcggc tccctgaagc cagtttgtgg tgagcatccc
    17941 gaggcggcga gctaaaaaca ccatttcatc agaaccggat ggtggcagga caagatcctg
    18001 ggcatggccg cggactattc tcagtccatc aatgagttgt cgaaaataca gatagtcgtc
    18061 tttgagctga gccccgtctt caagagtaaa caggccttcc ccgctcaggt gatcgatggc
    18121 ctccagggta ttgggcgtgc ggatggaagg atggtcgtgc ccgtgcatga gttggaaata
    18181 ttgcacggta tactccacat caagcaggcc accggctccg tatttgacat gcgtcgttcc
    18241 cggctggacc aactctttga tttgacgctc acgaagatgg agggccgtct gaagatccca
    18301 cgggtcggga gcatacacaa attggtcacg atgccgttca acggcttttc ctaccgcacg
    18361 atttccagca agaaaacgga gcttgagtaa cgcctgtcgt tcaaaaggag cggctccacc
    18421 ttccggcgca taatagcgct gaatttcctg gagggaattg gcgaggaggc ctttttctcc
    18481 atgcggccgc aatcgggtat ccaggtgaaa aatgccttcc tgcttcgctt caatccattg
    18541 gagaaattcc tgcgcccagc gttcaaaata atctgaggat ttctggtccg tctggcctcg
    18601 cgagggtttg cccggcgttt gatagacgaa cagaatttcg atatccgagg cataccctaa
    18661 ttcgccgcct ccgagttttc ctaatccaaa aatcgccatc gatggaggag cggagaggga
    18721 ggctttcgga ttgatcgcct gttgagaatg cagaagggcc tggttgagaa tgacatcagc
    18781 aagattggtc agggcctggg aaaagtcggg gaggggacta ttttccagga gatgacgcat
    18841 gtcgattcga aacagctctt cgtctttcca ttgattcaaa cgttgttttt tgtggacagg
    18901 agttttggct ttcagcaata acggctcgat ggccctggtg agtaccgatt ttgatcgaat
    18961 cagggggcct ttttgatatt gattcatcat gggcaacaaa ttgtcatgtt gccgtcggag
    19021 taaatcctcc cacaggtaat cactgctgcc aaagagttga gccagtcgtt ccagcgtgga
    19081 agcctggctc agcttggtga gatgcgaggg ggtattcgcc tgctctaacc agagatccag
    19141 gaattgatca aaatgatcga gcgctttccc ggggtccggt gcccacctca gataatgggt
    19201 gaattctttg agtaaggtgg ccgccgttcg tagttcctgt tgttcgcctt ttccctgaat
    19261 ttttccgcct tggcgtccgc ggacgtaaag acggttgcgg acgcggtggt tggcgacttc
    19321 aatcttggct ttcactatat agatcccgcg catggccagg gcattggcaa aggtgtacaa
    19381 aaacgccggc gtatcggttg agctaatatc cagaatggtt tcatgaccgg cgcgcgggtt
    19441 ggaaaacgtg atgttgaccg gatggaccaa ctccgtcggt ttttgtcgca tgttttctaa
    19501 attctcaacg agccggcgat tcacctgtcg acgggcctgc cggatctgat tcttttgaag
    19561 aaggacgaga agggccgtca ccatttcttg aaactcacgt tgttcgggtt cggcaaattt
    19621 tagatcttca agagcctgca cctgaaagac atccacgaca attttccggg ttaatcctcg
    19681 agtcgcttgt gaggaggaac ccgtggaggt atggccttgc aatgatgctg ttgagggaaa
    19741 agttggagac atcttttcta aagacgtaaa aatcgtggcc tctcgaatat ccagcccaaa
    19801 ggaggataag acgccacaga atgtggcaaa ctcggaaaaa taatcgtaac ccaccagcgt
    19861 cagttgaaat tgccgggtgg gcagggtccg gatcgagatc gcacagggtc gttcaaacgt
    19921 gagctggttc gccagggcta aatgttccag gatcgcggcg ggaggaaaac tttcaaaata
    19981 atccgggtcc atctgctgga caaaatctct gatcaaggcc ggacgaagat caggacattg
    20041 ggtttgatac gactggatcc gttctgcttc ggtcatggtg cccgagtata ctgaaaaaat
    20101 gttggattgt aaggtcggac gcgctaccgt ataattttct tttttcacga tgaccgcttt
    20161 caaaccacac gcagcttccg aggaggatcc tcccccagcc cgccttcccg atcccacgcc
    20221 tttactcctg gcttcaggac ttatcacgca agacgtggcc cggatacttc aggcctatgg
    20281 cctacaggat tggaaacgag ccgaccagaa ccttcaggcc atggccgggg aaccggtcac
    20341 ccggaaagcc ctggcgacca ttctgcccgc cgtgttacgc agtgttgcgc gtacggcgga
    20401 tccggatcag gccatgaatg aatgggaacg gtatgtcgac tccggtatcc aacggcttca
    20461 actctttcaa tatttagcga tggtcccaca tgtggtcgaa gtgatgggcg cggcattcgg
    20521 aaatagtccg gccatggcgc aaacgtttat ccgtgatccg ttactcgtgt attggatcga
    20581 ggatgacggg gtcctgcgcc ggcggatgac ccggattgcc cttgaggcca acgtgcaggc
    20641 cgccctcggt accgtgacga gctttgaggc gaaatgtgaa gcattacggc ggatgaagcg
    20701 gcgcgaaatg ctccgaatcg gaattcgcga tttgttgggc atcgccacgc ccgtagaaac
    20761 ctataccgtg ttgtcggatc tcgcctccgt agttattcag gcggtgtatg aactggtgag
    20821 ccaggaatta actcagcggt ttggcgattt tggaggaggg ggatcgaccg ggaaaaaggc
    20881 tgacggtttt tccgtgctgg ccatggggaa actcggaggc tgggagctga attacagctc
    20941 ggatgtcgat cttatttatg tgtaccatgc gccagaaggg aagacggaag cggggagggg
    21001 acaaaccagt attgctcgtg tgctgtattg tgagactctg gccagggaac tgacggccgt
    21061 cttgacggcg ccgacagccg aaggcgcctt gtttcgagtc gatctccgtc tccgtccgga
    21121 aggaatggtc gggccgttgg cctcctccct tgaggatgcc cttcattatt acgcgggtcg
    21181 aggccgaacc tgggaacgtc tggcctttct caaagccaag cccattgccg gcaatctccg
    21241 tgtggggcag gccttaatta aggggctgac agactttgtg tatgggaatg aagaagacga
    21301 agggcaggtc tttccggcta ttcattcgct ccggtcacaa atcatttcca aaatgattcg
    21361 ccggggggaa gtcgaccggc atgtcaagct gggcatcgga ggcattcggg aaatagagtt
    21421 tattgtgcaa gcccttcaac tgcgttgggg acaggcgtat cctaaaatac gcgatcggca
    21481 aacactcaaa gcattggtga agttgacgca cattgagaaa ctgacggcac aggacgctcg
    21541 cctgctaaaa gagtcgtatg tgtttctaag aaatcttgag aacaaacttc aaatggttca
    21601 tgaatttcaa acgcatctgc ttccgaacaa gtccgaagag attgccaaat gtgccgtcag
    21661 aatgggttac ctcaaacagg ccaccgttgc ccaggccatg gaagcgttcc tgagcgacta
    21721 tcgtcgacat accaccaggg tgcatcagtt gtacgaacgc attctgtgct cgtcaccctc
    21781 ctagcaaatc cgggatatac ctttttcaca cttcgttttc tctgggtgtt tctagcagca
    21841 ggaggaatgt caggtgtgaa tcagtccaga ggggttgaaa acgatccagg gattgttatc
    21901 tcttatagta agaagaaaat tttacatcta ttcattcttt tttcggggag gagctaatgg
    21961 accttaaccg gatgacgatc aaattacagg aagccctgca ggctgcctcg gggatcgcga
    22021 tgcgacgaag ccatcaaggg attgacgtgg agcatctcct gctggccttg ctggagcaat
    22081 ccggtggatt ggctcacccg atattagagc atacgggtgt ttcgcccacc gccgtcaaaa
    22141 atgccgcaga acaagcgctt cagaaggttc ctcaggttca gggcagtggg gcacctccag
    22201 ggcaggtgca tctctctcct cgcctggcaa aggtgctcaa tcaggcggaa acggagatga
    22261 aggaactgcg ggatgactac ctgagtgtcg agcatgtcat cctggccatg gtagccgaag
    22321 gaggcgtctt tacgtccatg ggactcaagc gggataaggt gctcgccggt cttcaggagg
    22381 tgcgaggcaa tcaacgcgtc accagccagg atccggaagg gacctatcaa tcactagaaa
    22441 aatacgggcg tgatctgacc cgattggcca gccagggcaa attggatccg gtcatcggcc
    22501 gggatgaaga aattcgtcgg gtcgtgcaaa tcctgtcccg ccgcaccaag aacaatccgg
    22561 tcctgatcgg tgaacctgga gtgggaaaaa cagccattgt cgaaggactg gcacaacgga
    22621 tcattaaagg ggatgtgccc gatggactta aaaataaacg cttgatcgtg ctcgatatgg
    22681 gtgccctggt agcgggtgcc aaatttcgcg gggagtttga ggagcgatta aaagccgtcc
    22741 tcaaagaaat tcaatcctcc cagggacaga tcctgttatt catcgatgag ttgcatacag
    22801 ttgtgggggc cggagcggca gaggggtcca tggatgccgc caatttgctg aagccgatgt
    22861 tggccagagg ggaactccat ctgattggcg ctaccactct tgatgaatac cggaaacata
    22921 ttgaaaaaga tgcggccttg gagcggcggt tccaaccggt cgtggtcgat cagccttcgg
    22981 tggaagatac catctccatc ctgcgcgggt taaaggagcg gtatgaagtc catcatgggg
    23041 tgcgcattaa agattcggca ttagtggcag cctcccgctt atccaatcga tatatcggtg
    23101 accggtttct gcctgataaa gcgattgacc tcattgatga ggcgagtgcg cgcttacgga
    23161 ctgaaatcga cagcatgccc gccgaaatgg atgaaatctc ccgtaaagtt ctgcaattgg
    23221 aaattgaacg ggaagcctta aagaaagaaa ccgatgcggc gagtcgcacc cggttgacta
    23281 caatcgagca ggagctaaaa ggcaaacagg aagccctgaa agacttaaaa acccagtggg
    23341 atgccgagaa atcgtcggtc gggaaattac aaacgatccg gcaggagatc gaaggagcca
    23401 aacaacaaat cgagcaggct gagcggcagt atgacctgaa tcgtgtggcg gagttgcgct
    23461 atgggacatt gccaaaactg gaaaaatcca tgaaggagga agaagagcgg ctggccaatc
    23521 agcagggcac cagccgtctg ctcaaagaag aagtggatga agatgacatt gccgaggtgg
    23581 tcagtcgatg gaccggcatt cctgtcagcc gattacttca aggcgaagtg gaaaagctgc
    23641 tgcatctcga tgaatggtta cacaaacgtg tcatcggtca ggatgaagcg gtcaaggcgg
    23701 tggccgaagc cgtgttgcgt gcccggtccg gcatcaaaga tcccaatcgc cccatcgggt
    23761 cgttcctctt cctgggcccc accggtgtcg gcaaaaccga gttgg
//
LOCUS       DAORAQ010000033        22472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_430_length_22472_cov_67.319222, whole genome shotgun sequence.
ACCESSION   DAORAQ010000033 DAORAQ010000000
VERSION     DAORAQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 22472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22472
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_430_length_22472_cov_67.319222"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(446..1687)
                     /gene="coaBC"
                     /locus_tag="PKM72_05075"
     CDS             complement(446..1687)
                     /gene="coaBC"
                     /locus_tag="PKM72_05075"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNP60189.1"
                     /translation="MSTGLTGKRIVLGVTGSIAAYKAVGLVRLLTQAGAGVHVVMTDS
                     ATRFIAPLTFEVLSGNPVASDVFAGHQDMKHLSLTEHADLLVVAPCTANTLAKMALGL
                     ADNLLGTLSLTVQCPVMVCPAMDGEMWAHPAVQAHAQTLRHRGVVLVEPEVGALASGE
                     WGQGRLAAEEVIMSTVVDLLHRRTDWQAERVLISAGPTQEPIDPVRFISNGSSGKMGF
                     ALAEAAAARGAEVVLVTGPTQLKAPKNVTVIPVVTAEDMLQALTARFEWATTLCMTAA
                     VGDFRPKQAHAQKVKKDQWHGEGLDLERTPDILMALSAQRTHQRLIGFAAETDHLIEH
                     GQDKLANKHLDMLIVNHIGGEQSAFGNDTNEVYVLTPGAAPHHIPRMPKRHLADMLLD
                     RIVSLPVQPSRQQKTLSSPTS"
     gene            complement(1715..2086)
                     /gene="rpoZ"
                     /locus_tag="PKM72_05080"
     CDS             complement(1715..2086)
                     /gene="rpoZ"
                     /locus_tag="PKM72_05080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNP60190.1"
                     /translation="MDALSLLPEHHQKEFDSRYRIVLIAAQRAKQLVRGGEPYGASKF
                     SKDTSRALEEVLNKQVPYLIGQEAKEAIKNAKRANERPIDPALYAQPDENAQEIKKEL
                     SVYIDDTAQHLGTVVEPEEQS"
     gene            complement(2166..2750)
                     /gene="gmk"
                     /locus_tag="PKM72_05085"
     CDS             complement(2166..2750)
                     /gene="gmk"
                     /locus_tag="PKM72_05085"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNP60191.1"
                     /translation="MLFVVSGPSGVGKSTLCRHILKTIPDIRLSVSYTTRKPRSGETD
                     GKEYRFISETEFHAKISENAFAEYAEVYGRLYGTPWKELEQESGSNTDILLDIDVQGA
                     RQVMKNLQKAVTVFILPPSLEVLRSRLVERATDTSDEQERRFQKSQEEMRSYSEYQYT
                     IRNETLEQAIEELQAVIMAERVRTTHVDATHVFR"
     gene            complement(2805..3683)
                     /locus_tag="PKM72_05090"
     CDS             complement(2805..3683)
                     /locus_tag="PKM72_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNP60192.1"
                     /translation="MKSMTGFGKRESLCQGTMVGVEVRSVNHRFCEMMVRLPKSLSHM
                     ELELKEQVKRSCERGRIELTVTMNGGGSGANKTVQLDRAMARRYVQGLRELQREFKLG
                     GTVDVNVVAGFRDLFSTSEEPTVIEDVPKVVEGLVQRALTDLEKMRKKEGTALQKDLL
                     QRVQIVEERLHVVQQRIPSALQVAADRLKARVAKLLEGERVNDDRIAQEIAMLAERSD
                     VTEELTRLQSHVAQFRSTLKSKGAVGKRLDFLLQEMGREVNTIGSKASDSEISGQVVD
                     LKSELEKIREQVQNIE"
     gene            complement(3680..4381)
                     /gene="nth"
                     /locus_tag="PKM72_05095"
     CDS             complement(3680..4381)
                     /gene="nth"
                     /locus_tag="PKM72_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNP60193.1"
                     /translation="MRGLASMSRLRTARHPIESPVEAKKRVGRILAALAKSVPDVRVE
                     LDSSNRLELLVATILSAQCTDQRVNQVTPNLFARYRTAQDYANADPAELEALIRTTGF
                     YKNKGRHLIGCGQALVQRFHGMVPGTMEDLTTLPGVGRKTANVILGSSVGEPAIVVDT
                     HVKRVANRLGLTKSQDPTKIEEDLQRLLPKAQWTVGAQRLLLHGRYVCLARTPKCAHC
                     VIASDCGWEGKQKLL"
     gene            complement(4378..6027)
                     /gene="hflX"
                     /locus_tag="PKM72_05100"
     CDS             complement(4378..6027)
                     /gene="hflX"
                     /locus_tag="PKM72_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNP60194.1"
                     /translation="MERLYRRRIPPEKVVTPEVARECAELTYETRRQIGLLVNRQGQV
                     ESVLIGDHHELVIPHLSRSRSSLRLLRGVRLVHTHLNNQPLTQDDLTDLALLRLDLIM
                     ALGVGKDGSLRDAYVAHILSNPIQGRTTLELPPCAFHSFQLDCQQFIQSLEQEIVRSN
                     PGLREGVREGRALLVSVSTGNAASEEGRLQELQELVRAQDVEVLGCITQRLGAIHPKF
                     VVGSGKMKELAIRALQSGADTLIFDQDLTPAQVRGISEITDLRVLDRSQVILDIFAQR
                     AHSSIGKIQVELAQLRYLLPRLAQSSTALSRLAGGIGTRGPGETKLETDRRRARERIQ
                     RLERDLESMAHGRHEQRKMRMKRGLPIISIIGYTNVGKSTLLNALTKSQVSVKDRVFE
                     TLDTVNRRWYLPGLGDVILTDTVGFIRDLPKDLMAAFQTTLLELQEADVLLHVIDAHV
                     PDPGQHIIAVDNVLKELGLENIPSLRFFNKADLMVEEDVQLLCQRYGGMGGSALQDAS
                     LLMIQQELTTLLRGLQAEGGRKGNSHEMPLYPLPATAETFP"
     gene            complement(6112..7152)
                     /gene="tsaD"
                     /locus_tag="PKM72_05105"
     CDS             complement(6112..7152)
                     /gene="tsaD"
                     /locus_tag="PKM72_05105"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNP60195.1"
                     /translation="MILGIETSCDETAVAVLDGDGHILSNILDSQVEVHARYGGVVPE
                     LASRRHMECLELLTREALDLAKVSLSQLTGIAVTNRPGLVGALLVGVNFAKALAYATN
                     IPWVTVNHLEGHLASAWLSDPNLPTPTMVLIASGGHTHLALVPHRGEYQFIGWTMDDA
                     AGEAFDKGAKMLGLPYPGGPAIDRLAKQGNAQYVSFPRPTLRSQNFNFSFSGLKTALR
                     YFVRDEQQKGSSPLPVADIAASYQEAIVDVLVEKLCRAARQYDVKGVSVVGGVAANSR
                     LRLRLEERARTLGLHVTLPHRALCTDNGAMIAAAGLEKLKRKERAAWDVDAISTLQCK
                     YDESAMAGVLSQ"
     gene            complement(7261..9690)
                     /locus_tag="PKM72_05110"
     CDS             complement(7261..9690)
                     /locus_tag="PKM72_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNP60196.1"
                     /translation="MFERFTDRGRKIIILAREEAERHQNDYLGTEHLVLAILRESDGI
                     ALMIIKKMGLSPEQIRLEIERNLPGGGTTVTFGEIPFSPRVKKVIEYGVEEARLLGHN
                     HIGSEHLLLGLLREEEGIGGKVLRSLGANLLTARQLTVTFLRKSGTRERDKKSSTPAL
                     DEFGRDLTQLAQEGTLDPVIGRSDEIQRVLQILSRRTKNNPALIGESGVGKTAIVEGL
                     AQRIIGLDVPDNLLNRRVIALDLGSLVAGTKYRGQFEERLKVVMKEISQAGNIILFID
                     ELHTLVGAGAAEGSIDAANMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPI
                     YVQPPSVEETVQIIQGLRDRYEEHHGVEITDEAVEEAVKLSERYITDRFLPDKAIDLI
                     DETGSRAKLLTYALPPELKSLEQELKRVAREKEVAIGLQNFEEAVKFREEEERFRKLL
                     DDSKRDWKKKQEKNKPVISGEDVAYVISKMTGIPLFKLEEEETEKLLHMEDYLHKRII
                     GQEEAISAVCRSIRRSRAGLKDAKKPIGSFIFLGPTGVGKTELARALAEFLFNTDDAL
                     IRVDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTEKVRRRPYSVVLFDEIEKAHPDIF
                     NVLLQVLDDGVLTDSLGRKVDFKNTILVMTSNLGTRFIQKGVSLGFQRDVEEEHTSRM
                     RGEVLAELKRHFSPEFLNRIDELVVFHQLTKEHLVSIVDILIGELNARLVERGVYLDV
                     TDEVKQWLIEEGFEQQYGARPLRRTIQRRLGDPLSEELIKGRFKDAKKIKVVLTDQTP
                     NFIEEEVLASV"
     gene            10005..10859
                     /locus_tag="PKM72_05115"
     CDS             10005..10859
                     /locus_tag="PKM72_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNP60197.1"
                     /translation="MSTFQTIGIITKPNAPNILQEISHLRDWLVSHHKTVILDSTQGF
                     PTSDNPTRIQIAKEADLLIVLGGDGTMLSGARLVEQRGIPILGVNMGGLGFLTEINFD
                     DLPTALEKVFAGECQLDKRLMLKVQLQRGDQILYTYSALNDVVISKGHLARMISTKIW
                     VNHAFMTNLRGDGLIVATPTGSTAYSLSAKGPILDPQLEVLLINPICPHTFSHRPFLA
                     PGDVPITIELTSQEPAMATIDGQVGMEILTGDLVQVRASDHRTHLIRFPERSYFEVLR
                     TKLKWGDG"
     gene            complement(10962..11324)
                     /locus_tag="PKM72_05120"
     CDS             complement(10962..11324)
                     /locus_tag="PKM72_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60198.1"
                     /translation="MSDLNPAQDYFCASETERTLKDLRLKRKGQPVYVMGHEDRYKGK
                     EGVFEFFNVRLAVVKFPDEKTLGFDPIDLLLPCEINEDGVPFFEIRYCETCDQVFPLT
                     SSEFHAPVERKECPECAP"
     gene            11462..11812
                     /gene="arsC"
                     /locus_tag="PKM72_05125"
     CDS             11462..11812
                     /gene="arsC"
                     /locus_tag="PKM72_05125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNP60199.1"
                     /translation="MADILIYHKPTCSTCRQVIKLVEESGQPFTTINYYEKTFSKSTL
                     KGLLKKAGIKSSEIIRKKEAIYKNLNLQDSQFTDDELLDILIKHPDLIQRPLVQKGDQ
                     AILARPPETVSKIL"
     gene            complement(11848..12468)
                     /locus_tag="PKM72_05130"
     CDS             complement(11848..12468)
                     /locus_tag="PKM72_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNP60200.1"
                     /translation="MALNEHNRRKFVAGMSRRISKLRDQSEQSLDFAIETMFQERTEA
                     YLHVEQGLMEVDRMLGLVEGELEDITELTTAQRLESRLEFIEDRFEEFESEIRQRPRR
                     RRHKINLFNFFKAAGGGGGEGEPTSVRGEIRSSVHAYEVLGVEFGSPLPVVTRAFRER
                     AKKIHPDIRQGDRSSEPELRRIIEAYQFLKTDYFGGTASDSVQRPF"
     gene            12736..13242
                     /locus_tag="PKM72_05135"
     CDS             12736..13242
                     /locus_tag="PKM72_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNP60201.1"
                     /translation="MNHYRLILGSSFILATMFAPPAWSANPAGYGTAGGEFNIKISRV
                     PSNEIAKAKSAKPPIEPTPEILAEGKEIFLGRGGCISCHGAEGKGDGPAAKNLPIQPR
                     NFTNPKFNTYRTPGEMMWVLKNGSMGQTGRVPGTGMLPVVQPKGFISEKDGWKVLLYE
                     RSLGNQQK"
     gene            13325..17041
                     /locus_tag="PKM72_05140"
     CDS             13325..17041
                     /locus_tag="PKM72_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNP60202.1"
                     /translation="MSESTTEHKPNRLSHETSPYLLQHAYNPVDWFPWGEEALNMAKK
                     ENKPILLSIGYSACHWCHVMERESFENQAIAELMNRYFVNIKVDREERPDLDEIYMQA
                     TTAMNQGHGGWPMTVFLTPDQDPIFAGTYFPPTDRYGRPGFGSILTNIGEGWKKDQSN
                     ITQQASRFTTRLRTALQPASPLAVGAAEIENAVKQFARDFDARYGGFGRAPKFPPATG
                     LSFLLRQYHWSLEKKILAMVTKTLDGMAAGGLYDHIGGGFARYSTDDEWLAPHFEKML
                     YDNALLARTYVEAFQVTGENRYRQVATETLDYILREMTAPEGGFYSATDADSEGVEGK
                     FFVWTPEQIRELITNEQDATRFCAYYDITEEGNWEHTNIPRTTKSLETVAEELGCSTE
                     DLRDTISRVRPEVYQARLKRIPPGLDDKIITAWNGMMIGTMAEAGRVFNHQPYLDGAI
                     RAADFLLTTLSRPESRLWRTYRSGHAHLNACLEDYAYAAEAMIDVYEASGQERYLHEA
                     VSLAERILEDFEDRDNGGFFTTPTDHEALIIRGREGADGATPSGNAVAASALARLSFH
                     GDREDFRKAATNAIRAYGQQIGQVPRGFPKSLMVVDLLLRGPIELALVGIPGDKLYDQ
                     LRSAVNACFVPYRILAYKQELNGEPTHPLLAGKTLANGKSALYVCKNFACQAPITDPQ
                     EVVDALSHPQGTTTSHTKEPPLQALTTQGLSGHATPQGTGQYVARILSSPQDSRPSPH
                     GYTSLGSTGLTTSRIGFGGYRIDVGVEDHRRALEKSLQGGCNLIDTSTNYADGGSERL
                     VGVVLKDLVAKQRISRDEIIVVSKIGYVQGNNITRAEAREQAGKPFPEMVKYGEGIWH
                     CLHPSFLEEQLILSLDRLGLETLDVCLLHNPEYFLSDAKNRNLSIDPLRLEDLRQEFY
                     RRLQEAFTYLETQIAAGRLQYYGISSNTCTAKPENPEATSLSRMLKAAEAAAKHAGLP
                     HHHFRVLQLPMNIFESGALLTPNTGPEQSQTVLAFAQEADIAVLVNRPLNAIPGKGGG
                     MIRLADPQAEISNTNFDAQHPKVAALEHDYKQVLAPQVPKPEKGAAPLDYFNWAEELK
                     RIRPSIHNLEHWDQIESQTIAPHANQVFQLLTRHFAGKQEEEQIWESWRDRYVPELVS
                     LLKVMRMEAAHKSAKKLSEIRKLIDPLIPDSKREEPFARKAIWAVASTPGVTSVLNGM
                     RHPVYVDDSLGIMKWEPLSQTRALFEAIHTSEIP"
     gene            17128..17889
                     /locus_tag="PKM72_05145"
     CDS             17128..17889
                     /locus_tag="PKM72_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNP60203.1"
                     /translation="METIGPLIGLGAIAGVFPIYLGIILALVIAKAMSRAWEGYLTGI
                     ATGVLVYLFFDLMHEAVEFTGATDPLSWVAFLGSLLIGFVGLVLIEQRQQGRNRTEPS
                     KLFLPYMIALGMGLHNLGEGLAIGAAYMQGEWVLSGVLIMGFALHNGTEGFAIIGSAG
                     KSRPSFKDIILMGLIAGLPTCLGTLISGFAVSHYFTIMFFALAAGSLLYVIFSLTTIF
                     YTATRRVQSAYGVFTGISLMFLTAMVLQIVSGIRT"
     gene            complement(18133..18861)
                     /locus_tag="PKM72_05150"
     CDS             complement(18133..18861)
                     /locus_tag="PKM72_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNP60204.1"
                     /translation="MANQEQLKLIKVGVDPWNAWRKANPSNRVDLSGGNFSKMELSGV
                     DLAGANLQDVNFNHADLSGAILSGANLTESSLLETNLTGANLENANVSDADLLRAILI
                     QANLERAHLAKTDLTGANLTKANLTEANLHLANLQGAKLTEANLEGCKLREANLDQAD
                     LAGANLGRADLAQANLKQANLLGANVEKASVIQVDFQEANLQTLANMTLLQLSKAKSL
                     RHAQFDPLIYEQLVREYPHIGEQD"
     gene            complement(19131..21692)
                     /locus_tag="PKM72_05155"
     CDS             complement(19131..21692)
                     /locus_tag="PKM72_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNP60205.1"
                     /translation="MILLGVLSYLTGLWLGPFLTLFPLSLLGALFSFGCILTWFEQQK
                     RVTRQTGFILFALVIGGIGHAHWASTAQLDSNLVVVDEEQSIFFEGTIVAPVRHTPDG
                     LILLVEATRLVRQGKEQVAQGRIRLTWRDPGEYSLIYGDHIAFMARVREPYGTMNPGG
                     FHFGQYLKRQGIHAVATVQGPEAIRVQDAQKRTTRELILGVVDRWRQTIHHAAVSSLR
                     NPALGLFLGMVLGEQSYIDSDIRDAFMASGTVHILSISGSHLGLLALLIFAGARWTFR
                     HLPIFWLERLSLRLTATRFAVLITLPAVSFYTLLAGAEMATVRSWIMIVVCCVGIWLG
                     RERNLVTALAVAALLMVLPHPEAIQDISFQLSYLSVAAIALVVLVRKQKESNLSGLDE
                     VFPPAQPTWLVGLWYKVVLAWLITLAVSLATLPLVAHDFHQIPWLGLFANMVIVPLVG
                     ALMIPLGLLSGMLVLMGGGGTLPLASVNQWVFDWLAQVVLMLSHIPGATWYVASPSLS
                     SMIVFWGMLAGVVALWHRHLFRWGCGTILLAILLWWGWSPRTGWDPGTVRVTFLDVGQ
                     GDATLIELPDGQTVLIDGGPSYRRLDMGRAVIGPYLWNQGIRRLDHVIATHPQWDHVG
                     GLPWVLDTFKVGQYWDNGVSRPEQFFSRLHLAVKSAGLREQILSAGTVIVSSGPCSLI
                     VLSPSRSERAERYSSWTVSSGKDLNNRSLVTRLECGNHSFLLTADVEQQSLDDLLHVS
                     NGFSARIVKVPHHGAKSSLNREWVNRLQAEAMVVSVGAHNRYGHPAPEVLDAYKKRGL
                     PLYRTDRDGAVWFTATPGLDDMTVRTAMEESLLQVQAGPGMWREEWANWTRIRNR"
     gene            complement(21955..>22472)
                     /gene="glmM"
                     /locus_tag="PKM72_05160"
     CDS             complement(21955..>22472)
                     /gene="glmM"
                     /locus_tag="PKM72_05160"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNP60206.1"
                     /translation="RHNTVVGTVMSNFGFEVCMREAGINLIRTAVGDRYILERMVAED
                     YNLGGEQSGHMIFLDYHTSGDGMISGLQMLKLMQRAQKPLSEIAMCMHSTPQVLVGVT
                     VPRKPDLQTLPQLQQAIQDKERLLNGTGRILVRYSGTEPLLRVMVEGQDSMVIKEIAE
                     DLITVVKSCIQ"
BASE COUNT         5799 a         6169 c         5013 g         5491 t
ORIGIN      
        1 aagagcgggt cgatgggatt caaatccgag ccattcccta agattccctg gacagcaatc
       61 ggtcttgtgg ccccattccc tgacacgtcc ctgttttgaa ggtttcaaag aagaaccaag
      121 tccttctatt gaacggggat gggggagagc agttagattc ttcggatagc cagaagaacc
      181 atggaaatga gccaggcaaa aaagacaata ttaatgaaaa atccttccat actattctta
      241 tcggcctatg agttcctaaa gttaagtttt ttcttgttct ttgagattct tgcttcattt
      301 ttcactcgtt atcgatatcc tgccgcacaa tccatgatga cccttggtgg ccattcacca
      361 cacaaccaaa gccctccggc tttggcgatt tcgttcattc ggaatggaaa aggatgggaa
      421 gacaccccca tcaggtgagc acaaattacg acgtcggtga agacagagtc ttctgctgcc
      481 tggaaggctg aactggaagg gacacgatac gatctaagag catatcggca aggtgtcgtt
      541 tcggcattcg tgggatgtga tggggggcag cacccggcgt aagaacatac acctcattgg
      601 tatcatttcc aaatgcagac tgttctccgc cgatgtgatt cacaatcagc atatccaaat
      661 gtttgttggc aagcttgtcc tgtccatgct caataagatg gtcagtttca gccgcaaagc
      721 caatcaggcg ttggtgtgtc cgctgggcgg agagtgccat taatatatcc ggcgtccgtt
      781 ccaaatccaa cccttcgcca tgccattggt cttttttgac tttctgtgca tgcgcctgtt
      841 tagggcgaaa atctcccacc gcagccgtca tgcagagcgt ggtcgcccat tcaaatcgtg
      901 ccgtgagcgc ctgcaacata tcttctgcgg tgaccaccgg gatcacggtc acattcttcg
      961 gagcttttaa ctgcgtggga cccgtgacca agacgacctc ggccccgcgt gcagcggcgg
     1021 cctcggccaa ggcaaatccc attttacctg atgacccgtt actgatgaat cgcacaggat
     1081 caatgggttc ctgcgtggga cccgccgata tcaggacacg ctccgcttgc caatctgttc
     1141 ggcgatgcag gaggtctacc accgtcgaca tgatgacttc ctcggcagcc aaacgcccct
     1201 gtccccattc tccggaggcc aacgccccaa cttccggctc aaccaggacc acccctcgat
     1261 ggcgcagagt ctgtgcatgg gcttggaccg ccggatgggc ccacatttcc ccatccatcg
     1321 ccggacagac catcacggga cattgaacgg taagcgaaag ggttcccaat aaattgtcgg
     1381 ctaacccgag ggccattttg gctaacgtgt tcgcggtaca gggagccacg accaacaaat
     1441 cggcatgttc cgttaaggac aggtgcttca tgtcctgatg gccggcaaag acatcactcg
     1501 ccaccggatt tcctgacaac acttcaaacg tcaggggggc gatgaacctg gtcgctgaat
     1561 ccgtcatgac cacatgaacc ccggcaccgg cttgagtcaa caaccgtaca agtcccactg
     1621 ccttataggc agcaatactt cccgtgactc caaggacaat ccgcttccct gttaatccgg
     1681 tgctcatcct gatggaatct cccgccaagt caattcagct ctgttcttca ggttcaacca
     1741 cggtgccaag atgttgagcc gtgtcatcga tatacacact caattctttt ttgatttctt
     1801 gggcattttc atccggctgt gcatagagag ccggatcaat gggacgttca tttgctcgtt
     1861 tggcattttt aatggcttct ttggcttcct ggcctattaa atacgggacc tgtttgttca
     1921 gcacttcttc caaagccctc gaggtgtctt ttgaaaattt gctggctccg tagggctctc
     1981 caccccgaac caattgcttg gcccgctggg cagcaatcaa tacgatccga taccgtgaat
     2041 caaattcttt ttggtgatgt tcgggtaata acgataatgc atccattgga tatcctcttt
     2101 cctttctgtg aaaaacacct gatcagatga atagtctaca caaatcaatc ccatcaatgc
     2161 agcatttatc ggaacacatg tgtggcatcg acatgggtgg ttcgaacgcg ttcagccata
     2221 atgacggctt gaagttcctc gatggcctgc tccagagttt cattgcgaat cgtatattgg
     2281 tattccgagt aactccgcat ctcctcttgg gatttctgaa accgacgctc ctgctcgtcc
     2341 gatgtatccg tcgcccgttc aaccagacgg ctccgaagga cctccaaaga aggaggcaga
     2401 atgaataccg tcacagcttt ttgaagattt ttcatgacct gccgcgcgcc ctgcacatcg
     2461 atgtccaaca agatatccgt gttcgaaccg gactcctgct ccaactcctt ccatggtgtt
     2521 ccgtataatc gtccatagac ttccgcatat tcagcaaacg cgttctcgga aatttttgcg
     2581 tgaaattcag tttccgaaat aaacctgtac tccttgccat ccgtttcgcc ggaccgtggc
     2641 ttcctcgtgg tatacgacac cgaaaggcga atgtcgggaa tggtcttcaa aatatgccgg
     2701 cagagcgtcg acttccctac cccggaagga cctgatacga caaataacaa tttcttcccc
     2761 tttattgaca acgtttctcc cacgttatga catggctgga ccgattattc aatattttga
     2821 acctgttccc gaattttttc taattcactc ttcaaatcca caacttgtcc ggaaatttcg
     2881 gaatcgctgg cctttgatcc tatggtattc acttccctcc ccatttcctg gaggagaaaa
     2941 tcaagccgtt ttccaacggc acccttggat ttgagtgtgg agcgaaattg tgccacatga
     3001 ctctgcaacc gtgttaattc ttccgtcacg tctgatcgtt ccgccaacat ggctatttct
     3061 tgagcaattc gatcgtcatt cacccgttcg ccttctagta acttggcgac tctggctttc
     3121 agacgatcag cggccacctg cagggcagaa ggaatgcgtt gctgaactac atgaagccgt
     3181 tcctcgacta tctggactcg ctgtaagaga tctttttgta aggcagtgcc ttctttcttg
     3241 cgcatttttt ccaaatcggt caaggcacgc tggactagtc cttcaacaac cttcggcaca
     3301 tcctcgatca ccgtgggctc ctcactggtg gaaaaaaggt cccgaaaccc tgccaccaca
     3361 tttacatcca ccgttccacc cagcttgaat tcccgttgta gttcgcgcaa cccttgaaca
     3421 taccgcctcg ccatggcgcg atccagttga acagttttat tggctccaga ccctccccca
     3481 ttcatggtca cggtcaattc aattcgaccc cgctcacacg aacgtttgac ttgctccttg
     3541 agttccaact ccatatgaga aagagactta ggcagacgca ccatcatctc gcaaaaccga
     3601 tgattgacgg atcgaacttc aaccccaacc atggttccct gacagaggga ttctcgtttt
     3661 ccaaaaccgg tcatactttt cataatagct tttgttttcc ttcccatcca cagtctgaag
     3721 cgatcacaca atgagcacac ttgggcgttc tggccagaca cacataccgc ccgtgaagca
     3781 ggaggcgctg ggctccaacc gtccattgcg cttttggcaa aagccgttga agatcctctt
     3841 caatttttgt cggatcctgg cttttcgtca agccgagccg attggctacc cgtttcacat
     3901 gggtatctac cacaatggca ggctctccca ccgaactccc taaaataaca ttggccgttt
     3961 ttcgcccaac gccaggcagg gtagtcaagt cctccatcgt ccctggcacc attccgtgaa
     4021 aacgttgcac cagtgcctgg ccacacccaa taagatgacg ccccttattt ttataaaacc
     4081 ccgttgtccg aatgagagct tctaattccg ctggatccgc attggcataa tcctgtgccg
     4141 tgcgatagcg ggcaaagagg ttcggcgtga cctggttgac tctctggtct gtacattgcg
     4201 ccgaaagaat cgtggccact aacagctcaa gccggttgga tgaatctaac tctaccctga
     4261 cgtcgggaac ggatttagcc aatgccgcaa gaatccggcc tacccgcttt ttcgcctcca
     4321 ccggcgattc aatcggatgt cgtgctgttc tgagccggct catcgaagca agacccctca
     4381 tggaaaggtc tcggccgtag ccggaagagg atagagaggc atttcatgag aatttccctt
     4441 cctgccacct tccgcctgaa ggccacgaag aagagtcgtg agttcctgct gaatcatgag
     4501 caatgacgcg tcttgtaacg ccgatccccc catgccgcca tatcgctgac aaaggagttg
     4561 gacgtcttct tcgaccatca gatccgcctt attaaaaaaa cgcaggctgg gaatgttttc
     4621 aaggcccaat tccttcaaca cattgtcaac tgcgatgata tgctgcccag gatccggcac
     4681 atgggcatct atcacgtgga gtaacacatc ggcttcctgc aattccagca aggtggtctg
     4741 aaatgccgcc ataaggtctt ttggcaaatc tcggatgaat cctacggtat ccgtcagaat
     4801 gacgtcgcct aatccaggta gataccatcg ccgattgacc gtatcaaggg tttcaaagac
     4861 gcgatctttt acagagactt ggcttttcgt caacgcattt aatagagtcg acttccccac
     4921 attggtatag ccaatgatgg aaatgatggg aagccctcgc ttcatacgca ttttccgttg
     4981 ttcatgacga ccatgcgcca tagactccaa atcccgctcc aagcgctgaa tgcgctcacg
     5041 ggctcgacgg cgatccgtct ccagcttggt ttctccaggt cctcgggtcc caattcctcc
     5101 cgctaaccgg gaaagagcgg tactggattg agcaagtcgt gggagcagat accgcaattg
     5161 tgccagctca acttgaattt tcccaatgga ggaatgcgcc cgttgtgcga aaatatcgag
     5221 aatgacttgt gatcggtcca agacccgcag atccgtgatt tccgaaatac cccgaacctg
     5281 ggctggagtc aaatcctgat caaatatcaa ggtatcggca ccggattgca gcgcacgaat
     5341 tgccaattct ttcatcttcc ccgaaccaac cacaaacttt ggatgaatcg ctcctaaccg
     5401 ttgagtgata caccctaaga cctcaacgtc ctgggcacgc accaattctt gtaattcctg
     5461 gaggcgccct tcttcagaag cggcattccc tgtgctgaca ctgaccagga gggctctccc
     5521 ctcccgaaca ccttctcgta agcccgggtt cgaccgaaca atttcttgct ccaatgattg
     5581 aataaattgc tggcaatcca attgaaacga gtgaaaggca caaggcggta attccaacgt
     5641 agttcgcccc tggatgggat tcgaaagaat gtgcgccaca taggcatcac gaaggctgcc
     5701 atcttttccc acaccaagag ccatgattaa atccagccga agaagagcga ggtcggtcag
     5761 atcatcctgg gtcagcggtt gattattgag gtgtgtatgg actaaccgaa cccctcgtaa
     5821 taatcgaagg ctcgaacggc tcctcgaaag gtgagggatg accaattcgt ggtggtcacc
     5881 gatcaagacc gattcaactt ggccttgtcg attgaccaaa agcccgattt gccgtctcgt
     5941 ctcataggtc agttcagcac attcccgagc cacctctgga gttacgactt tttctggagg
     6001 gattcgtcga cggtaaagac gctccattcg gctaatatga ttagccttta acccatgtag
     6061 attacctagg acaacaggaa taactcttct ccttttgccg tatcatcgga gtcattgaga
     6121 caggactcca gccattgcgg attcatcata tttacattgc aacgtagaaa tcgcatccac
     6181 atcccatgct gccctctcct tgcgcttgag tttttcaagc cccgcagccg caatcatggc
     6241 accattgtcc gtgcagagcg cacggtgagg tagagttaca tgcagcccta atgtgcgagc
     6301 acgctcttca agcctcagac gaaggcggga gtttgccgct actcctccca caactgagac
     6361 ccctttcaca tcatattgac gagcggcgcg gcaaagtttt tccaccaata catccacaat
     6421 cgcttcctgg taactggccg ctatatccgc tacgggcaag ggagagctcc ccttttgttg
     6481 ttcatcccgc acaaaatatc gaagcgcggt tttcagccca ctgaaactaa aattgaaatt
     6541 ttggcttcgg agtgttgggc gagggaagga tacatattga gcattcccct gtttggcaag
     6601 ccggtcaatt gccggaccac caggataagg aagcccaagc attttggcgc ctttatcgaa
     6661 cgcctcaccc gcagcatcat ccatcgtcca tccaatgaac tgatattctc cacgatgagg
     6721 caccaatgcc aaatgggtgt gcccaccaga cgcaatcaaa accatggttg gggtgggcag
     6781 attcggatcg gacaaccatg ccgacgccaa atgcccctcc aaatgattaa ccgtcaccca
     6841 gggtatattg gtggcatagg ccagagcttt ggcaaaattt actccaacaa gtaaggcccc
     6901 taccaatcca ggacgattgg tgaccgctat tcccgttaat tgggaaagag aaaccttggc
     6961 cagatccaat gcttcccttg tcaaaagttc caggcattcc atatgacgac gcgaagccaa
     7021 ttccgggacc actcccccgt aacgggcatg cacctcaacc tgcgaatcca atatatttga
     7081 gaggatgtga ccatccccat ccaacacggc cacagccgtc tcatcacacg atgtttcaat
     7141 tcctaaaatc acggatagag tccttttcag caccaagatt cattgggtaa aaaacaacaa
     7201 aaccctcagg actgatggtc ctgagggttt catctaggat ttgaccatcc ctatttccaa
     7261 tcaaacactc gctaacacct cttcttcaat gaaatttgga gtttggtcgg tcaggacaac
     7321 cttaatcttt ttggcatctt taaaccgccc cttaatcagt tcttcagata gaggatctcc
     7381 caggcggcgt tgaatagttc ggcgtagagg acgtgcacca tattgttgtt caaagccttc
     7441 ctcaatcaac cactgcttca cctcgtcggt gacatctaaa tatactcctc tttccacaag
     7501 acgggcattg agttccccaa tgagaatgtc aacgatactc accaaatgtt ccttcgtgag
     7561 ttggtggaag accactaatt catcaatacg atttaagaat tcaggactga aatgacgctt
     7621 caactccgcc aggacttcac ctcgcatacg tgaagtatgc tcttcttcaa catcacgctg
     7681 gaacccgagc gatacacctt tttgaatgaa tcgggtaccc aagtttgacg tcatcaccag
     7741 aatggtattc ttgaagtcca cctttcggcc taaactgtcc gtcaataccc catcatcaag
     7801 gacctgcagc aacacattaa atatatccgg atgggctttt tcaatttcat caaataacac
     7861 aactgaatag ggtcgacgtc ttactttctc cgttaattgt ccaccttcct catagcctac
     7921 atacccagga ggggcgccaa acaggcgtga actggtaaat ttctcctggt attccgacat
     7981 gtctacccga attaacgcat catcagtatt gaacaagaat tctgctaatg cgcgagccaa
     8041 ctccgtcttc cctacaccgg tcggtccaag gaaaataaac gacccaatgg gttttttggc
     8101 atcttttaat ccagctcgcg atcgacgaat agaccgacaa accgcagaaa tagcctcttc
     8161 ctgccctatg atgcgcttgt gaagataatc ttccatatgc agcagctttt cagtttcttc
     8221 ttcttcaagt ttaaagagag gaatccccgt cattttcgaa atgacgtagg ctacgtcctc
     8281 accggaaata accggtttat tcttttcttg ttttttcttc cagtcacgct ttgaatcatc
     8341 taacaatttt ctgaaccgct cttcctcttc gcgaaatttt acggcttctt caaaattctg
     8401 aagcccgatt gccacttcct tttcacgagc gacccttttg agttcctgtt caagagactt
     8461 caactcagga ggaagggcat acgttagcaa ttttgctcgc gaaccggtct catcaataag
     8521 atcaatggct ttgtccggca aaaaccggtc ggtgatatac cgttcactca atttcacagc
     8581 ctcttcaact gcctcatccg taatttcaac accatggtgt tcctcgtatc gatcacgtag
     8641 cccttgaatg atctgcacgg tttcttctac ggaagggggc tgaacataaa taggctgaaa
     8701 ccgccgcttg agtgccccat ctttttcaat atgctttcta tattcatcca gggtcgtggc
     8761 gccaatacat tgaatttcac cacgagataa agccggcttt aacatgtttg cggcgtcaat
     8821 tgacccttca gcagcacctg caccgactaa ggtgtgaagt tcatcaataa agagaatgat
     8881 attccccgct tgggaaattt ctttcatcac caccttaaga cgctcttcaa attgccctcg
     8941 atatttggtc cctgctacta aggaacctag atccaaagca ataacccttc gattcaataa
     9001 attgtccgga acatccagac cgatgattcg ctgggccagc ccttccacaa tagctgtttt
     9061 accaactccg gattcaccaa ttaacgccgg gttatttttc gtacgccgac tgagaatttg
     9121 aagaaccctt tggatttcat ctgaacgtcc gataacaggg tctagcgtcc cctcctgagc
     9181 caactgtgtc aaatccctgc caaattcatc taatgcaggt gtgctactct ttttatctcg
     9241 ctccctggtt cctgatttcc taaggaacgt gacggttaat tggcgagccg ttaaaagatt
     9301 ggctcctaaa ctacgaagca ccttcccccc tattccttct tcctcacgaa gaaggccaag
     9361 gagcagatgt tcactgccta tatgattgtg gccaagaagc ctggcttcct caacaccata
     9421 ttcaatcact tttttcactc tggggctaaa tgggatttcg ccgaatgtca cggttgttcc
     9481 accccctggc agattccgtt cgatttcaag acggatttgt tccggcgaga gacccatctt
     9541 tttgatgatc atcaaggcta tgccgtccga ctctcgaaga atggcgagca ccaaatgctc
     9601 ggtacccaga taatcgtttt ggtgccgttc cgcttcttct ctggcaagga tgatgatttt
     9661 tctaccacga tccgtgaatc gttcgaacat tggacctcct ctagccgatt attcgcgaaa
     9721 cttttcgctc acacattcgt ctattatcca caaataaatt cgagaaaaat tcacaatttc
     9781 agtctatccg tgatccctca aaactgtcaa gtcagaagcc ggattgagag gacccagcaa
     9841 cggtaatgat gatttactct accaatcaaa atacgctaag aaaagcctcc tgccacacac
     9901 ggatattatt gcctatacca tttccaaaaa atccgcgctt gctatcagtt caatgaagac
     9961 actaccatcc tgacgcctgg caacaccatt tcttatcgaa gctcatgtct accttccaga
    10021 ctatcggaat catcaccaag cctaacgccc ctaacattct gcaggaaatc tcccatctcc
    10081 gtgattggtt ggtttcacat cacaaaactg tcattcttga ttccactcaa gggttcccta
    10141 cttctgataa tcccacccgt attcagattg ccaaggaagc cgaccttctc attgtattgg
    10201 gtggcgatgg cacaatgtta agcggagcac ggctcgttga acaacgagga attcccatac
    10261 tgggagtcaa tatgggaggt ctgggtttcc tcactgaaat caactttgat gatctcccca
    10321 cagccttaga aaaagtgttc gcaggagaat gtcaactgga taaacgcttg atgctaaaag
    10381 tccagctaca acggggtgac caaatcctct acacctattc agccttaaac gacgtggtaa
    10441 taagtaaagg acatttagcg agaatgatct ccaccaaaat atgggtgaat catgcattca
    10501 tgacaaactt gcgtggggat ggactgatcg tcgctacccc gacgggatcg acagcttatt
    10561 ccctttcggc aaagggtccg attttagatc cacagctgga agtattacta ataaacccaa
    10621 tttgcccgca taccttttcc caccgcccct tcctcgcccc tggcgatgtg ccaatcacca
    10681 ttgaactgac aagtcaggaa ccagccatgg ccaccattga tggccaagtg ggaatggaaa
    10741 tcctgacggg tgatcttgtg caggttcgag catcagacca ccgtacacac ctcattcgtt
    10801 ttccagagcg aagctacttt gaagtgttac gcacaaaatt aaagtggggg gatgggtaaa
    10861 tcctgccttc aagattcccc taaaaccgac acaaccacat tcatgtctat cccatttgcc
    10921 agcctggcaa cctgccggaa attccctgtt tcaaaaggga cttatggtgc gcactccgga
    10981 cattctttcc gctcaaccgg agcatgaaac tccgatgaag tgagaggaaa aacttgatcg
    11041 cacgtttcgc aatagcgaat ttcaaaaaaa ggtacgccgt cctcgttgat ctcacaaggt
    11101 aataataaat ctatgggatc aaatcctagc gttttttcat cggggaactt taccacggca
    11161 aggcgaacgt taaaaaattc aaatacccct tccttgcctt tataccgatc ttcatgaccc
    11221 atcacataca cgggctggcc tttacgttta agccgaagat cctttaaggt tcgctccgtc
    11281 tcagatgcac aaaaataatc ttgagctggg tttaggtcgc tcatactggc aaaatccctc
    11341 ggtcgtttaa attcatcaga ctgttccttc cgcctacagc tggaccagct tgtagcatgg
    11401 cctcaaaagg aggtcaaggc ggtgaaagaa ttgacagacc atttacaaag gaggcgtatc
    11461 catggctgac atcctcattt atcacaaacc cacttgctcc acttgccgac aggttatcaa
    11521 attagtggag gaaagcggac aaccctttac gaccattaac tactacgaaa aaacattttc
    11581 caaatccaca ctcaaagggc tgctcaaaaa ggcggggatc aaatcgtcag aaattattcg
    11641 aaaaaaggaa gcgatttaca aaaatctgaa cttacaagac tcacaattca ctgatgatga
    11701 attattagac attcttatca aacacccaga tctcatccag cgtcctctgg ttcaaaaagg
    11761 ggaccaggca atcctcgccc gccctccgga aacagtcagc aaaattcttt aacgaaatcc
    11821 tcgcggggga aaaagcctgg ccaaaagtca gaatggccgc tggacagaat ctgatgccgt
    11881 accgccgaaa tagtcggttt tgaggaattg gtaggcttcg ataattcgcc gcagttccgg
    11941 ctcggaactg cggtcgcctt gtcggatatc aggatgtatc ttcttcgccc gctcacgaaa
    12001 ggctcgagtt accactggca aaggacttcc aaactcaacc cctagcactt cataggcgtg
    12061 gacggacgaa cgaatttctc cacgaacaga agtgggctct ccctcccctc ctccacctcc
    12121 ggcagcctta aagaaattaa agagattgat cttgtgccgt cgacggcggg gacgctgcct
    12181 tatttcgctc tcaaactctt caaaacggtc ttcaataaat tctaaccggg attccaagcg
    12241 ctgggcggta gtcagttcgg taatatcctc caactccccc tcaaccagcc ccaacatccg
    12301 atcaacctcc atcaacccct gctcaacatg cagataggcc tcagttcgct cttgaaacat
    12361 tgtttcaata gcaaagtcca agctttgctc agattggtcg cgtagcttcg aaatccgccg
    12421 actcatgcca gccacaaatt tccgtcgatt gtgctcgttt aatgccataa tgccaaagct
    12481 caaggaaaat ttttggccaa ttgtaacaca actgtccact aagcaagggg actgttatta
    12541 ctcgaaaaga gagaatgccg actcaattaa aacaagtgat taaaggcggg gtttgcccct
    12601 caccttgatg attggtatcc tatttaccct tgagcgcata accttagaag atcatacatg
    12661 gtgaagaacg cgagtgattt ccacaatatt aaatttcttt ctgcctatcc aaaatttgca
    12721 ttgaaggaga tttgcatgaa tcactaccgg cttattttgg gaagtagctt tatcctcgcc
    12781 accatgtttg cgccacctgc ctggtctgcc aaccctgctg ggtatggtac cgcaggcggt
    12841 gaattcaaca tcaaaatctc ccgggtccct tcgaatgaga tcgccaaagc caaatcggcg
    12901 aaacctccca ttgagcccac accggaaatc ctggcggaag gaaaagaaat ctttctggga
    12961 cgtgggggtt gtattagttg tcatggggca gaaggaaagg gggatgggcc ggcggcaaaa
    13021 aaccttccca ttcaaccgag aaatttcacc aaccctaagt ttaacaccta tcgaacacct
    13081 ggtgaaatga tgtgggtcct caaaaacgga agcatgggac agaccggccg ggtccccgga
    13141 accgggatgc ttcctgttgt tcaacccaaa ggattcattt cggaaaagga tggctggaag
    13201 gtgttgctgt atgaacgaag cctcggaaac caacaaaaat agtatttctc ctaaattatg
    13261 tattgctaga tagtactacg tctccaattt ttattatcct gatcacgttg agtgaaaaac
    13321 ctttatgtct gaatccacga cagagcacaa acccaatcga ctgagtcacg aaaccagccc
    13381 ttacctcctt cagcatgcct acaatccagt agattggttc ccctggggcg aggaagcgct
    13441 gaatatggca aagaaagaga ataaaccaat tttgctctcc attggctatt cagcctgcca
    13501 ttggtgccac gtcatggagc gggaatcgtt tgagaatcag gccatcgcgg aactgatgaa
    13561 ccggtatttc gtgaatatca aagtggacag ggaagaacgg ccggatttgg atgaaatcta
    13621 tatgcaggcc accactgcca tgaaccaggg tcacggtggg tggcccatga ccgtattcct
    13681 cacccccgat caggatccga tttttgcagg aacctatttt cctcccacgg accgctatgg
    13741 tcgtccggga ttcggatcaa ttctgacaaa cattggggaa gggtggaaga aagatcaatc
    13801 caacatcacc caacaagcca gtcgattcac cacgcgtctg agaaccgctc tacaacctgc
    13861 atctccgctt gccgttggtg cagccgagat tgaaaatgcc gtcaagcaat ttgcccgtga
    13921 ttttgatgca cgttatgggg gatttggacg agcgcccaag tttccgcccg ccaccggact
    13981 ctcatttctc ttgcgtcagt accactggag tctggagaaa aaaatactcg ccatggtcac
    14041 caaaactttg gatggcatgg ccgcaggggg actgtacgat catatcggcg gcggatttgc
    14101 gcgctattcc actgatgatg aatggttagc cccccacttt gaaaaaatgc tgtatgacaa
    14161 tgccctcctc gcccggacct acgttgaagc ctttcaagtc acaggggaaa atcgataccg
    14221 ccaggtcgcc acggagacct tagattacat tttgcgagaa atgaccgcac cggaaggcgg
    14281 gttttattca gcaactgatg cggacagcga aggagtagaa gggaagtttt tcgtgtggac
    14341 gccggaacag atacgtgaac tcataacaaa tgaacaagac gccacccgct tctgcgccta
    14401 ttacgacatc accgaggaag gcaattggga acacaccaac atcccgcgca caacgaaatc
    14461 actagaaacg gtcgcagagg aattaggctg ttccaccgag gatctacgag acacgatcag
    14521 ccgtgtgcgc ccggaagtct accaggcacg tctgaaacga atcccacctg gacttgatga
    14581 taaaatcatc accgcctgga atggcatgat gatcgggaca atggccgaag ccggtcgagt
    14641 atttaaccac caaccctatc tagatggtgc catccgtgcc gccgattttc ttctgaccac
    14701 actttcacgt ccggagagcc ggctgtggcg cacctatcgg tcagggcatg cccatctcaa
    14761 tgcctgtttg gaagattatg cctatgcggc cgaagccatg atcgacgtct atgaagcctc
    14821 tggccaggaa cggtaccttc atgaggcggt ctcgttagct gaacgtatcc tggaagattt
    14881 tgaagatcgg gacaacggtg gattttttac cacccccact gatcatgaag ccttaattat
    14941 ccggggacgg gaaggcgccg atggtgcaac tcccagtgga aatgcggtgg ccgcttcggc
    15001 tttggcccgc ctatcatttc acggtgatcg tgaagacttt cggaaagccg cgaccaatgc
    15061 tattcgtgcc tatggacaac aaatcgggca ggtgcccaga gggtttccca aaagtctcat
    15121 ggtcgtagac ctcctattac gaggccctat cgagttggcc cttgtaggaa ttcccggcga
    15181 taagctatac gaccaactcc gttcagcagt gaatgcgtgt tttgtgcctt accgcatttt
    15241 ggcctacaag caggagctga atggtgaacc gactcatccc cttttggccg gaaaaactct
    15301 ggcgaatggg aaatccgcgc tgtatgtctg taaaaacttt gcctgtcagg ctccgatcac
    15361 cgatcctcaa gaggtcgttg acgcattgag ccatcctcag ggaaccacca cttcacacac
    15421 gaaagaacca cccctgcaag cactgaccac ccagggcctt tccggacatg ccacccctca
    15481 aggaacgggg caatacgtag ccagaatcct ttccagccca caggattctc gcccttctcc
    15541 tcatggatat acgtctttgg gttcaaccgg attgacgact tctcgcatag gctttggtgg
    15601 ttaccggatc gacgttggag tggaagacca ccgaagggct cttgaaaaat ctttacaagg
    15661 cgggtgcaac cttatcgata cgtccaccaa ttatgccgac ggtggaagcg aacggttggt
    15721 cggtgtggtg ctcaaggatc ttgttgctaa gcagaggatc tcccgcgatg aaatcattgt
    15781 ggtctcaaaa atcggctatg tgcagggaaa taatattaca cgcgccgagg ccagggaaca
    15841 ggccgggaaa ccgtttcccg aaatggtcaa atatggcgag ggcatctggc attgtctcca
    15901 tccttcattt ctagaggaac aactcattct gtctcttgac cgactggggc ttgaaaccct
    15961 ggatgtctgt ctcctgcaca atccagaata ttttttgtct gatgccaaga accggaactt
    16021 atcaattgat ccactcagac ttgaggatct ccggcaggag ttttatcggc gtctgcaaga
    16081 agcttttacc tatctggaga cacaaatcgc cgccggccgt ttgcaatact atgggatctc
    16141 ctccaatacc tgcacggcca aacccgaaaa tcccgaagcc acatcacttt ctcgtatgct
    16201 caaggccgct gaggctgccg cgaaacatgc cggcctccct caccatcatt ttcgtgttct
    16261 gcaactccca atgaatatat ttgaatcggg agccctgctc acccccaata ccggacctga
    16321 acagtcgcaa acggtcctgg catttgccca ggaagccgac attgctgttc tggtgaaccg
    16381 gcctctgaat gccattccgg gaaaaggcgg aggcatgata cgtctcgcag atccccaagc
    16441 ggaaatttcc aatacgaact ttgatgccca gcacccaaaa gtcgccgcgc tggaacacga
    16501 ttacaagcag gtgctagcac cccaggtccc caaaccggaa aaaggagctg ctccactgga
    16561 ctatttcaac tgggccgaag aactgaaacg aattcgtccg tccatccaca atcttgagca
    16621 ctgggaccaa atcgaatcac aaacgattgc ccctcatgcc aatcaggtct tccaactact
    16681 caccagacat ttcgccggca aacaggaaga agaacaaata tgggaatcgt ggcgggatcg
    16741 ctatgtccct gaactcgtct ccctcctcaa ggtcatgcgc atggaggccg cgcataaaag
    16801 tgcgaagaaa ctctctgaaa ttcgtaagct catagacccc ctcataccgg attccaaacg
    16861 ggaagaacct tttgcccgta aagccatctg ggcagtagcc agtaccccag gtgtaacctc
    16921 tgtgctcaat ggcatgcgac atcctgtcta tgtcgatgac tcgctgggca tcatgaaatg
    16981 ggaaccgctc tctcaaaccc gagcactatt tgaagccatt catacatccg agattccctg
    17041 atccttgatc ccatgttatt tataatatga aaatgaaacc aagtttcatt attgccaaca
    17101 aactctaata tttgataggt ttgtttcatg gaaacaattg gaccattaat cgggcttggc
    17161 gcaattgcgg gagttttccc gatttacctt ggcatcatcc tggccttggt catcgcaaag
    17221 gccatgagca gagcctggga aggctattta accggaatcg ccaccggtgt gcttgtctat
    17281 ctcttttttg acctcatgca cgaagccgta gaattcaccg gcgcgacgga tccgctatcc
    17341 tgggttgcct ttctcggaag tttattgatc gggtttgttg gtctggtgct cattgagcaa
    17401 cgccaacagg gtcggaacag aacggagcca tcaaagctgt tcttacccta tatgatcgcc
    17461 ttaggaatgg gcctccataa cctgggcgaa ggcctagcga tcggcgccgc ctatatgcaa
    17521 ggtgaatggg tcttaagtgg tgtcctcatc atgggttttg ctttacacaa tgggacagaa
    17581 ggatttgcca ttattggctc ggctggaaaa tcaagaccaa gtttcaaaga tatcattttg
    17641 atgggattga tagcaggcct accaacgtgt cttgggactt tgatcagcgg attcgcagta
    17701 tctcactact ttactatcat gttttttgcc ttagctgcag gttcgctgtt atacgtcatc
    17761 ttttcattaa ccacgatttt ctacactgcc acacgccggg tccaatcggc ctatggggtc
    17821 ttcaccggta tcagtctcat gttcctcact gccatggttc tgcagatagt cagcggcatc
    17881 cgcacgtaag aaggcgacta gctcaaaccc agcactcctt gaaattccgc agccaacctg
    17941 ttgttcattg cggaccttct gaacgaccac cctcgttctg cttacaccat aaaaaatggc
    18001 cagtcttttc ctctcagatt aggaggtccc catcaaaact cacattatcc aacgttggca
    18061 tgacctttcg agaccccatc atatttttcc ctcacttgta gaaatttctt gatacctacc
    18121 aatgccgctt acctaatcct gttcacctat atggggatac tcccttacca actgttcata
    18181 gatgagcggg tcaaattgag catgccgcaa cgatttggct tttgacaatt ggaggagcgt
    18241 catgttggca agagtctgaa gattagcctc ttgaaaatcc acttgaatta ctgaagcctt
    18301 ttcaacattc gcacccagta gattggcttg cttgagattg gcctgcgcca gatcagcacg
    18361 accaagattg gcaccggcga gatctgcctg gtccagattt gcctctcgaa gtttacatcc
    18421 ttccaaatta gcctcggtga gttttgcacc ctgcaaattg gccaggtgaa gattcgcttc
    18481 agtcagattc gctttagtca gatttgcccc tgttaaatcc gtcttggcga gatgtgcccg
    18541 ctcaagattc gcctgtatga gtatggcccg gagaaggtcc gcgtcactca cattcgcatt
    18601 ttccaagttc gcgccggtta aattcgtttc caacaagctg gactcagtca aattagcccc
    18661 ggacaatatg gcacctgaaa gatccgcgtg attgaaattg acatcttgca agttggcacc
    18721 cgccaaatcg acaccggaca attccatttt ggaaaaattc ccaccactca aatctacgcg
    18781 attggaagga ttcgcctttc tccaggcatt ccagggatcc acacccacct tgatgagctt
    18841 caattgctct tgatttgcca tgagactcct tttaatgccg acaacccgtc ttgatacaat
    18901 attgtatccg aggcttaacc gtatatcctc tattctatgg tggcttaaaa caattaatac
    18961 aaattcaaca cacgtctctt cccaatatgc gggaaaagat ccaacccctc agcaattgac
    19021 ggattcgaga attctatcgt aaaacatccc tgatggaatc aaaagtgggt agaaggtcaa
    19081 gcatgaaaat gaccctgaag cccacatagc gagttatggg aaaaccctgt ttatcggttt
    19141 cttatccgag tccaattggc ccattcctct ctccacatac ccggaccagc ttgaacctgg
    19201 agcaggcttt cctccattgc tgttctcacc gtcatgtcgt ccaaaccggg agtcgcggtg
    19261 aaccagactg ccccatcccg atcagtccgg tataaaggaa ggcctcgctt tttatacgca
    19321 tccaaaacct cgggagccgg atggccatac cggttgtggg ctccaaccga aacaaccatc
    19381 gcttcagctt gaagtcgatt cacccactca cggttcagag aacttttcgc tccatgatgc
    19441 ggcactttca cgatgcgagc agaaaaacca ttggaaacgt gcagcaaatc atcaagagat
    19501 tgctgttcca catcggccgt gagcaaaaag gaatggttcc cgcactcgag tctggtaacg
    19561 agcgatcgat tattgagatc ctttccacta gacactgtcc aggaactata cctctccgcc
    19621 cgttcgctac gggaaggact cagcacaatc agagaacaag gtcctgagct gacaatgaca
    19681 gttcctgccg aaagtatctg ttcccgaagc ccggcggatt ttaccgccaa gtggagccga
    19741 gaaaaaaatt gttccggccg agagacgcca ttatcccaat actgtcctac tttaaaggtg
    19801 tccaacaccc atggtaatcc gccgacatga tcccattgcg gatgcgtcgc gatcacatga
    19861 tccagccgcc ggataccctg attccagaga tacggcccga tgaccgcacg tcccatatcc
    19921 aatcgtcgat aggatggtcc gccatcgatc aacacggttt gaccgtcggg taattcaatg
    19981 agggtggcat caccctgccc cacatccagg aacgtaactc gtacggttcc cggatcccac
    20041 ccggtgcgtg gcgaccatcc ccaccacagc agaatggcga gcaggattgt cccacacccc
    20101 catcggaaca gatgccgatg ccacagggcc acaaccccag ccagcattcc ccagaagacg
    20161 atcatcgaac tcaggctcgg cgatgcgaca taccaagtcg ctcccggaat atgtgagagc
    20221 atcaatacga cctgcgcgag ccagtcaaag acccattgat tgacactagc caagggaaga
    20281 gtgcctcctc cccccattaa gacgagcata cctgacaaca gacctagagg aatcatcaac
    20341 gcgccaacca agggcacaat caccatattg gcaaataacc ccagccaggg tatttgatga
    20401 aagtcatggg caaccaaagg caaggtggcc aaactgactg cgagggtgat cagccaggct
    20461 agcacaacct tataccagag acccacgagc caggtcggtt gtgccggtgg gaagacttca
    20521 tctaatcccg ataggtttga ttccttctgt tttcggacca gaaccacgag ggcaattgcc
    20581 gcaactgaga gatatgataa ctggaatgaa atatcctgaa tggcctccgg atgcggcaat
    20641 accatcagga gggcggcgac ggctaaggct gtcaccaaat tgcgttccct tcccaaccat
    20701 atgccgacac aacacaccac gatcatgatc caggaacgga ccgtcgccat ttccgcacca
    20761 gccagcaagg tataaaagga aacggcgggc aatgtgataa ggaccgcaaa gcgggtggcg
    20821 gtaagtcgta aggatagtcg ctcaagccag aatattggta aatggcggaa ggtccatcgt
    20881 gccccggcaa aaatcaacaa agccaatagg cctaaatgag atccagagat cgagaggata
    20941 tgcacggttc cgctggccat aaatgcgtct cgaatgtccg aatcaatata actctgttct
    21001 cctaatacca tccccaggaa taatcccaaa gcaggatttc tgagactgga cactgccgcg
    21061 tgatgaatcg tttggcgcca acgatccaca acacctaaga tcaactcacg ggtagtccgc
    21121 ttttgggcat cctgaaccct gatggcttcc gggccttgca cagtcgcgac agcatggatt
    21181 ccctgccgtt taaggtattg cccaaaatga aacccgccag ggttcattgt gccatagggt
    21241 tcgcgtactc gagccatgaa ggcaatgtga tccccgtaga tcaaggaata ctcaccgggg
    21301 tcccgccagg tgagacggat tcggccctga gccacctgct cctttccctg cctcaccaat
    21361 cgagtcgcct ccaccagcaa aatgaggcca tctggagtat gccggaccgg ggccacaatg
    21421 gttccttcaa aaaaaattga ttgctcctcg tccacaacaa ctagattcga atccaactga
    21481 gcggtcgatg cccaatgtgc atgaccgatt cctcctatca caagcgcaaa taggataaat
    21541 cccgtctgtc gagttacacg tttctgttgt tcaaaccagg taaggataca cccaaaacta
    21601 aaaagagccc cgagaagtga caggggaaac aaggtaagaa acgggccaag ccaaagcccg
    21661 gtgaggtagg acagaactcc aagaagtatc acataggaca gcgcatatgg ttaatgaagc
    21721 tcatcttaag gcctgctagg ttttccttac tcaaagaccc catatattaa gcgcagagta
    21781 tggccttcca ccgtctccag gagaccttgc ccataaaaaa agcaagggac ttaacgcaaa
    21841 tggttaagga tgggagaaaa gcaggccata ttgatgattg cgtctattct tcctaaatgc
    21901 caaatctttc attggagcaa acgactgaaa taatggtgag agaccctcta atccttactg
    21961 aatgcaggac ttcaccaccg tgattaaatc ttccgcaatc tccttgataa ccatgctatc
    22021 ctgcccttcg accatcactc gcagtaacgg ttccgtaccg gaatagcgta caaggatccg
    22081 tccggttcca ttcagcaggc gctctttgtc ttgaatcgcc tgctgtaact gtggaagggt
    22141 ctgcaggtcc ggtttacggg gaacggtgac ccccaccaac acctgaggcg tggaatgcat
    22201 acacatggca atttccgaca gtggcttttg agcccgctgc atcagcttca gcatttgcaa
    22261 gccggagatc atcccatcac cgctcgtatg ataatccaag aagatcatat ggccggattg
    22321 ttcgccaccc agattataat cctcagccac catccgctct aaaatgtatc gatcccccac
    22381 tgctgttcgt atcagattga taccagcttc tcgcatacag acctcaaacc caaaattact
    22441 catcacggtt cccaccaccg tattgtgtcg ta
//
LOCUS       DAORAQ010000034        22050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_442_length_22050_cov_65.942305, whole genome shotgun sequence.
ACCESSION   DAORAQ010000034 DAORAQ010000000
VERSION     DAORAQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 22050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22050
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_22050_cov_65.942305"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1139
                     /gene="rfaE1"
                     /locus_tag="PKM72_05165"
     CDS             126..1139
                     /gene="rfaE1"
                     /locus_tag="PKM72_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNP60207.1"
                     /translation="MKSLSVVPSISARKFKQYIQRFSRARILVVGDLILDHYVWGKVH
                     RVSPEAPVPIVHVDSESYRMGGAANVYHNILTLGGQAELCGVVGADHVGKQFLADIRQ
                     SSPFTSGVFVDSGRPTIKKTRVVAHNQQIVRFDVEQRHDISSQQTKKMIRHVASRLPG
                     ASCLVISDYAKGMITVELMKAIHTLAVQFNVPIVVDPKVEHMAYYQGVTVITPNHLEA
                     KQAAGFLPSQDVPVDEIGVALQQRLQCRAVVVTRGEEGMSIFENTGNSWSIPAVARQV
                     YDVTGAGDTVISTLALALSAKASLPEAAVLANQAAGIVVGMVGTATVTQAQLQEALLH
                     GHN"
     gene            1126..1929
                     /gene="kdsB"
                     /locus_tag="PKM72_05170"
     CDS             1126..1929
                     /gene="kdsB"
                     /locus_tag="PKM72_05170"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNP60208.1"
                     /translation="MATTDSLISLCIPARHGSSRFPGKPLALLAGKPLIQHVYEAVQQ
                     VSHVGQILIVTDQESIEEEVKKFGGEVCRVTDFCRTGTDRVAKVVSQLKYDVIVNLQA
                     DEIPQHPGLLDDLIHPFVSSPAGIGTLKRQIHTSQDLTNPSIVKVVTNIHGQALYFSR
                     SPIPCWRDGMTSGNNHIAYMHLGVYIFRKSDLLRFAELPTGYLEDAEKLEQLRALEHG
                     LPIMVWETEHQSIRIDTPEDIMAAEDLLSKKPFEPKVRPVGATQRVENR"
     gene            2010..3611
                     /locus_tag="PKM72_05175"
     CDS             2010..3611
                     /locus_tag="PKM72_05175"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNP60209.1"
                     /translation="MSRFLFVTGGVVSSLGKGLSSAAIGHLLESRGMTITFLKLDPYI
                     NVDPGTMNPYQHGEVYVTDDGAETDLDLGHYERFTTVQLHRENNCTTGRIYESVIQRE
                     RRGEYLGATVQVVPHITDAIKQTILNVAHDVDVVIVEIGGTVGDIESLPFLEAIRQMP
                     YEVGRENVLYIHLTLVPYIGTAGELKTKPTQHSVNKLREIGIQPNILLCRTDRFLPPG
                     VKDKIAMFCNVDKGSVITAKDVDSIYEVPIILRKEGLDELIVRSLHLETRPPNLRDWD
                     ILVQKIKRPRHEVTIALVGKYVESRESYKSVSEALTHGGLQDETAVHIHWVESGDIEK
                     DGPERLLADVDGVLIPGGFGLRGIEGKIDTIRYARDRNLPFFGICLGMQCAVIEIARS
                     LGGLQNANSSEFNPDTPYPVIDLLPEQRSIQEKGGTMRLGGFPCRLIPNTLTFAAYEQ
                     TEIRERHRHRYEFNNEYLEALTSKGLTISGTSPDGRLVEIIELQGHPWFVGTQFHPEF
                     QSRLCAPHPLFTAFIGAALQKKFGT"
     gene            3627..4475
                     /gene="kdsA"
                     /locus_tag="PKM72_05180"
     CDS             3627..4475
                     /gene="kdsA"
                     /locus_tag="PKM72_05180"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNP60210.1"
                     /translation="MAQPTQVDIGPFSVGGSSPHFLIAGPCVIESEKLVIETAAAIAE
                     IAKDLGIPYIFKASYDKANRTSVSSFRGLGITEGLKILKKINCELGIPILTDIHEVHD
                     VSQVAEVVDVLQIPAFLCRQTDLLSAAAKSGRVVNVKKGQFLSPWDMANVVHKLEDAG
                     TRKIFLTERGASFGYQNLVVDMRSLPVMRNMGYPVVFDATHSVQLPGGGGTVSSGQRE
                     FVAPLARAAAAAGCDGFFMEVHPRPEEALSDGPNMIRLSELRGLLEQIQAIWQVTGKG
                     EISPVT"
     gene            4504..5124
                     /gene="pgsA"
                     /locus_tag="PKM72_05185"
     CDS             4504..5124
                     /gene="pgsA"
                     /locus_tag="PKM72_05185"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNP60211.1"
                     /translation="MNQSEAVVKEKSERNNVIMGEAYLNLPNSLTLLRILLIPVFVWF
                     FSESSPERALAAGLVFACAAFTDFLDGYLARKSGQITNLGKLLDPVADKLLVASGLIL
                     LVQFQRVAVWLAIVMIAREMIVTGARVVAAKEGFVVPADSLGKFKVIGQIGGILCLIL
                     QGVWVQSEGLLATIGTGLLYIALFFSLFSGWRYLMQIFKKISPQYW"
     gene            5148..5744
                     /gene="plsY"
                     /locus_tag="PKM72_05190"
     CDS             5148..5744
                     /gene="plsY"
                     /locus_tag="PKM72_05190"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNP60212.1"
                     /translation="MEFYLLGVVGAYLLGSIPFGLVASRIFGKEDPRTRGSHNIGFTN
                     TLRVSGKKVGILTLIGDLGKGTVATVVAGSMGFPWLWILFIGFSVILGHVFSIFLRFK
                     GGKGVATALGAIIGIHPLIGWLLVGIWLGAVWGFGYSSGGALIAFGAFPFLVFLLTSD
                     FYFCIFSLGVMAIIFISHKENIHRLRQGTESKINLTST"
     gene            5787..7160
                     /locus_tag="PKM72_05195"
     CDS             5787..7160
                     /locus_tag="PKM72_05195"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNP60213.1"
                     /translation="MKLGHFSLVFSLSCHLLFIAPSLAISQTAKELRGLELLGDIEQA
                     ITSLAERVTPTVVNITPIHEIAQGQGFQRRAPFSKGSGSGVIVREDGIIVTNNHVVGE
                     DATEADVRLSDMSNMIAQVIGRDKETDIAVLKIQADRKFPAAHFGDSNALKVGQWVLA
                     VGNPMGLDRTVTLGVVSGIGRERLNLSRYENFIQTDAAINPGNSGGPLFNLRGEVIGI
                     NTAIIHMAQGIGFSIPADMVSRVVDQLVSQGRVVRGWLGVGIQSLTKELAEQFGIKEG
                     HGVLVNEVYEHDPAHVAGIKPGDIIVSVDDSPVDSPNQLSRLVARVGPGENAKILVLR
                     DGKEMTYLVPMAERQEKSMLASLPLQKSEVTLGLDVQGLTAALAEQFELQETVGVLVT
                     KVEPGGLANSEGVQEGDLINEVNRQTVRTVTQFSEAVAKVKPGQTVLLRIIRKTRAFF
                     IVIKTLP"
     gene            complement(7188..9290)
                     /gene="fusA"
                     /locus_tag="PKM72_05200"
     CDS             complement(7188..9290)
                     /gene="fusA"
                     /locus_tag="PKM72_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNP60214.1"
                     /translation="MPHESAPSIRNVVITSYPGVGKTTLCEALAFSTGVIPAMGTISQ
                     GNTIGDFEPEEVHRHHSISSAICQFEWQGTKINIIDTPGMSDYAFEIQSALRAVDGAV
                     LVVGASTGLRSEIERVWDLIQEHETPCLLFINELDKEGADYRSILAECEKTLGFTGVP
                     ITIPVGEGAMPTGVIDLISRNVITPLSGTTKIQQTEIVAEHQPIANEFRRRAMEQVAE
                     TNDQLVEKYLSQGEISDEDLRDGLTIGTLERKLVPVLCGSAIHNIGTTSLLAAVQRFL
                     PSPSDRSSLHPHVGQQPQTHDEGQRKSDPYDPFSAFVFKTTIDPFMGRLSFVRVLSGT
                     LQADSGFYNASRQTREKGGHLFFSIGKKHHQVHQVSAGDIVAIGKLKDTQTGDTICDE
                     RHSIIFPCLKISRPVMSYALEVKSKNEIDKVSLGLHKLVEEDPSLEFLRNEETKEMLL
                     SGVGQGHIEATLDKLRRKYGVEVDLHMPKVPYKETIHRMAQAQGKYKKQTGGHGQYGD
                     CWLQIEPLPRGTGFEFHNNIVGGVIPRNFIPAVEKGVIEALQHGIVAGFPIVDIRVAV
                     YDGSHHPVDSSEMAFKVAGSMALKHALDSAQSTLLEPIMSVEVLVPDDLVGTVIGDLN
                     SRRGRIQGMATKGHNQIVKAFVPLAEMLKYAPSLTSMTAGKGTYVMEFSGYEEVPKDC
                     LNRIVEEHKKEKEAVSSH"
     gene            9751..12021
                     /locus_tag="PKM72_05205"
     CDS             9751..12021
                     /locus_tag="PKM72_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP60215.1"
                     /translation="MSTLTPELAGMTSKNPFAGLQQLNVEVFPADFSDVRGHSPLPFS
                     LRCLGGTWEGYLIQWDEHNVRLHLTGFPPIELSQIIEWEYRISDQDHSLTTIQGMIKD
                     IHVETGHTAWEGTTNIFLHCSTSTGIRFLPNQGEGLVAYGHVKPEGSDSVSGFDPREQ
                     EKEFKAESIHPPKKGVRSNRLTIEKSNGQRIRAFHDRPIEGNPTEGPVVVIAPGYGET
                     KRDYLTLAYYFASNGFHVVRYDHTNHIGESDGLHHQICLSSMKDDFQTVTRYVRTNWP
                     ASAIIGVASSLASRVALKAEVECPSVSLLIMLMGIVNVERSAATVHQEDLFAGYGKGQ
                     CPESANFLGFNVGNHFLGDAIANQFVTLEHTLTDAASATSPVILVSAEKDAWVASDDL
                     QAFRNALGRRLEEWIVVPNALHRLQENPKIARETYRQLIAQCQNHIAQTPSSETIHNP
                     NRLDLGRQNRREKIAQQQQAETDVGAGFWQDYLGHFQSVAKCRDYVKLLDHAFHALGP
                     ITPGQRILDAGCGNGNAGLYFVNGLASTISPGISCLEESIQYVGIDVVPDALKRAHEA
                     MVAATTEWQHTKISSTPSVTKSWAQVDLGQPLPFPDNQFDRIVSNLVIGYVQDPGAAL
                     RELYRILAPGGRMVISNLKPNGDFSGIYQSLVSNAALPQQREEARDLLNNYGKIRQAE
                     KEGQFCFFDRTQWEWIINTLGGANAGIFSTFAGQAYLIVLEKPVVCAQAQRPMILNKK
                     QSPRLTPLPGALKQVA"
     gene            12266..14437
                     /locus_tag="PKM72_05210"
     CDS             12266..14437
                     /locus_tag="PKM72_05210"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60216.1"
                     /translation="MNFYAFSGLLNGLAATASGLIVYAKNPRSPKHQAYALYCLAAAI
                     WGYGFCAWQLGSSHDLALFFVRLLMVGAIFLPAAYLWHVLTLLDDVESNRWIIRLGWG
                     TSFIYFFANFTSYFVADVHPVGDFLFWPKPGPFFHFYLLGFGLSTIYGTWLLYHGARS
                     RTGPERSRFYLLTIGSVIAYAGGVTTFPLWYGIEIPPNGTILLTVYTSVVAYTLLRYR
                     LMDLGTAVERGITGSLLLALVAFPAYPILLLGQSLYFGEINIAYSFVELSVLLILVLG
                     ASSLKREAKTLITKSLFKERYDRHETVVHFSKSLVSILELKDLTATIVESICPTLGLE
                     WGVLYLRSSSSQDYRPVSSFGKSTNDLPVLYLQKTPTLLEMWRQGTSCFVINELELST
                     INSDSEAVIAQLADIGTEVCLPLVNKTRLLGFCVFGPNTKSGGGYTTQDLDLLTTLSQ
                     EASVALDNALLYEELKRSQSLVRRTDRLRSLETMAGGLAHEIRNPLTSIKAFVDLAPE
                     RKDDEQFLVRFSKVVKEDVFRIERLTREILDYAKPMEPFLKEEDLNDIVESCLYTLRI
                     RPSHELIVVETDLAHGLPKVFADRQQLKQVFLNLLFNAVEAMLPEGGVLTVRTRIIGS
                     GLAQNWVQVDIQDTGKGINPEDIEHIFDPFFTTKHLSQEHEGTGLGLAIAHQIIQEHR
                     GSIEVQSVRGGGATFLVNLPSSPAPTSVSPTQSVLPNPEHR"
     gene            complement(14596..>15327)
                     /locus_tag="PKM72_05215"
     CDS             complement(14596..>15327)
                     /locus_tag="PKM72_05215"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP60217.1"
                     /translation="GDYLGLMQRQWSEWSHLTEVIRVGQPWDSKEPETTEYRRSFSCA
                     MHHRSLQPAREVAQQISLKAARSLLDLGGGPGTYALAFLALNPILHATVMDRPAALDV
                     ARMLAEQSSLGNRLTCQAGDFLTARISGTYDVVWYSNVLHIYSPADNLKIFKKIKRIL
                     NPGGRLLIQDTFLEDQKELRPLEANLFAVSMLLYTEQGNTYSVRDVREWLQRAGLTRS
                     RVLHLKEGTGDWDGQLIEGRLPRTG"
     assembly_gap    15328..15427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15428..16182)
                     /locus_tag="PKM72_05220"
     CDS             complement(<15428..16182)
                     /locus_tag="PKM72_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP60218.1"
                     /translation="MAWSQSANLNSLGNQHFSKGFYTEAFQYYAQALEVDRQNGDQRS
                     LATTLGNLGNICAVSGRREQARAYYSEVLELQKILGDDRGISTTLANLGNLSADAGEW
                     ERAQAYYLEALDLMNQLSDYAGKAVLLSDLGLVARETGKEEEALAYYQESLILMRRVG
                     NEAGQSDVFRMMARLYLSQRRFEEAQSCTLTSLDVARRLKDELRMGGAWYVLASCHEA
                     QGQWNQAVQYLQKVVRIDQKYQLPKLTENTQRL"
     gene            complement(16246..16845)
                     /locus_tag="PKM72_05225"
     CDS             complement(16246..16845)
                     /locus_tag="PKM72_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60219.1"
                     /translation="MKTILPNISEWSWFCEEKQINFNGHLLAVGEHRILVDPPSMSAG
                     DMAMTQRGGSPDYIILTNRDHEREAANFRKTFKCQVMAPELDAKEMTLPIDKTFKDGE
                     LLPGGIWVVQLSDQKSPGESALFLQQGKGVLIVGDGIIGDPAGALRLLPPEKYENILL
                     AREGLRRLLKYTFDSLLVGDGTSILTGAKPALEDLLVDN"
     gene            complement(16842..17081)
                     /locus_tag="PKM72_05230"
     CDS             complement(16842..17081)
                     /locus_tag="PKM72_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60220.1"
                     /translation="MDELEDGKQKFLEIVNTLDSSVEVVIPTTPSRSLLLISLTKGPN
                     RKFITIHEDDMLDLPEESGVQTKITTLLQETLETL"
     gene            complement(17127..17681)
                     /locus_tag="PKM72_05235"
     CDS             complement(17127..17681)
                     /locus_tag="PKM72_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNP60221.1"
                     /translation="MKKNIHQVLIVAILILGTSFISHAGSFFKVGESAPNFSLTGLDG
                     QTLSLDQFKGKVVVLGLFHICEPCLIQSTNLQMVHEAIKDKPVAVIGVNSSGDSMADV
                     REFLQGFPVKVTYPYLLDPEKKTDKLYGGGKFIPNVYVIDQHGLIRWQRVGNFFTDGT
                     LAGHEAILQEVEKLLESPSTPSTM"
     gene            complement(17794..19245)
                     /gene="purF"
                     /locus_tag="PKM72_05240"
     CDS             complement(17794..19245)
                     /gene="purF"
                     /locus_tag="PKM72_05240"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNP60222.1"
                     /translation="MRKLPMVSDMPSSDGFHEECAVFGIAGSKEAANLTYLGLYALQH
                     RGQEGSGIVSSDGEQFFQKKGLGLVADIYSKKTLRELSGTKAIGHNRYSTAGSGQLQN
                     VQPLTVNFAFGNLALAHNGNLINAGVLRGELEAYGAIFQSDSDSEVIIHLIAHSKGDT
                     IVNRVIDALSLVRGAFSLVLLTDNHLIAARDPFGFRPLCLGRYKGSWVVASETCAFDL
                     IDAKFVREVEPGEIVVIHDSEMTSYHPFLERPHAQCVFEYVYFARPDSKIFGPHAVYP
                     VRKAFGRQLAKECPADADMVIPVPDSGVPAALGYAEGMGLPFEIGLTRNHYVGRTFIE
                     PEQAIRHFGVRLKLNPVPDVLEGKRIVVVDDSLVRGTTSRKIVKMLRQAGAREIHLRI
                     SSPPVIAPCFYGIDTPTQKELIGANLNIEEIRRYVTADSLGYLSLEGMLAMAPGFSNH
                     YCNACFTDNYPIMLTKAEQLQLGLFEPDQLSHS"
     gene            complement(19325..21598)
                     /gene="purL"
                     /locus_tag="PKM72_05245"
     CDS             complement(19325..21598)
                     /gene="purL"
                     /locus_tag="PKM72_05245"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNP60223.1"
                     /translation="MTQDSKASPHISLTSKIIEQHGLSEDEYQQILKHLGREPNLTEL
                     GIFSVMWSEHCSYKSSRIHLKRFPTEGERVVQGPGENAGAVDIGEGLAAVFKMESHNH
                     PSYIEPFQGAATGVGGILRDIFTMGARPIALLNSLRFGELDLPKNRHILKGVVSGISS
                     YGNCMGVPTVGGEITFNDIYSKNPLVNVFCLGIVKKDRLLRGLAKGAGNQVLYIGAKT
                     GRDGIHGATMASDSFDDASEKKRPNVQVGDPFLEKLLLEACLEFLEQDLLVGIQDMGA
                     AGLTSSSCEMASRAGTGIDMDLVKVPRREPHMTPYEILLSESQERMLLVAQPGKEEAV
                     LAICRKWGIDAAVVGRVTDDGFLRIREGHKIVAEIPAHALAEEGPRYERPAVPPQYQE
                     FLQSLNIDALPDVKDPAEVLLKLLASPTIASKRWVFRQYDHMVGTNTVVCPGSDSAVV
                     RVKGTSKALAMTTDGNSRFCLLNPYLGGGLAVAEAARNLVCAGALPIGVTDCLNFGNP
                     ERPDIMWQFIMAVEGIADACRTFEIPVVSGNVSFYNETNGLSIYPTPIVGMVGLIERP
                     ENIITQWFKQSGDQVLLLGETKEDLGGTEYLKVIHSREQGNPPWLDMDREKALHSCLL
                     SLIQDGLVQSAHDCSEGGLLVTLAECCLTHPSAPFGATVILTQDRLRLDALLFGESPS
                     RVVISIKPENIKRVRELVEGFGVPSIELGQVTQENMDITVRGGNDQPVSQISLSLSEM
                     GSAWHHSLARQLGTEES"
     gene            complement(21808..>22050)
                     /locus_tag="PKM72_05250"
     CDS             complement(21808..>22050)
                     /locus_tag="PKM72_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNP60224.1"
                     /translation="VKTTMEVFPGITMTSDICHGKPCVAGTVVDVATIVGTLGIGKSF
                     DDVQEALNLTYEQILTALRYASYVTDHLPLRLPSGH"
BASE COUNT         5697 a         5160 c         5310 g         5783 t
ORIGIN      
        1 ggtcatggaa tgggcaggga gcttggaccg attggatccg atgggaacga atcggaaagc
       61 tcattgaata gtgagcacac aatcacttga cccaaccaaa acttcagcct tgtactgatt
      121 caaggatgaa atcgctatcc gttgtcccat cgatttccgc tcggaaattc aaacagtata
      181 ttcaacgctt ttctcgcgcc cgcattctgg ttgttggaga tctcatttta gatcattacg
      241 tctggggcaa ggtgcatcgt gtctcccctg aagcgcctgt accgattgtt catgtggact
      301 ctgaatcgta ccgaatgggt ggggcagcga atgtgtatca taatattctg acattaggcg
      361 ggcaggctga attgtgtggc gtggtcggtg cggatcatgt tgggaaacaa ttccttgctg
      421 acatccgaca atcgtcccca tttacctccg gtgtgtttgt tgattccggt cgtcctacaa
      481 ttaaaaaaac gagagtggtt gcgcacaatc agcaaattgt tcgatttgat gtggagcaac
      541 gccatgacat ttcgtcccaa cagacaaaaa aaatgatacg acatgtggct tcccgacttc
      601 ctggagcctc ctgcctggtg atttctgatt atgcgaaggg catgatcacc gttgaactga
      661 tgaaggccat tcatacccta gctgttcagt ttaacgtacc gattgtggtg gatccgaagg
      721 tggaacatat ggcctattac cagggagtga cggtgattac acccaatcat ctcgaagcca
      781 aacaggctgc agggttcctc ccaagccagg atgttccggt tgatgaaatc ggcgttgctc
      841 ttcaacaacg cttgcagtgc cgggcagttg ttgtcacgag gggagaggag ggaatgagta
      901 tttttgaaaa tacaggcaat tcctggagca ttccggctgt ggcccgacag gtctatgatg
      961 tgaccggagc aggcgatacc gttatcagta ctcttgcctt agcactgagt gcaaaagcct
     1021 cacttcctga ggctgcggtc ttagccaatc aagccgccgg tattgtcgtg ggcatggtcg
     1081 gtactgccac agtgacgcaa gcacaacttc aagaggcatt actccatggc cacaactgac
     1141 tcactcatca gtctgtgtat tcccgctcgc catggatcat ctcggtttcc gggaaaacca
     1201 ctcgcgctct tagccggaaa gccacttatc caacacgtct atgaagcggt tcaacaggtt
     1261 tcacatgtag ggcaaatcct gattgtcacc gaccaggaat ccatcgaaga agaggtcaag
     1321 aagtttggtg gagaagtctg tcgcgtaacg gatttctgtc gaacaggaac ggaccgggtg
     1381 gcgaaagtgg tttctcaatt aaaatatgac gtgattgtca atttgcaagc cgatgaaatt
     1441 cctcaacacc ctgggctgct tgatgatctc attcatcctt ttgtgtcttc tccggcggga
     1501 atagggacct tgaaacgaca aatacacacc tctcaggatc taaccaatcc gtcaatcgta
     1561 aaggtggtga ccaatatcca cggtcaagcc ctgtattttt cacgaagtcc gataccctgt
     1621 tggcgcgatg gaatgacttc tgggaataat catatcgcgt atatgcactt gggagtgtat
     1681 attttcagga aatcggatct actacgattt gccgagttgc caacgggcta tcttgaggat
     1741 gcggaaaaat tagaacaact gcgagcctta gaacatggcc ttcccataat ggtgtgggaa
     1801 acagaacacc aatctattcg aatagatact ccggaggata tcatggctgc ggaagatctt
     1861 ctatcgaaga aaccctttga gccgaaagtc agaccggtgg gggcaacaca gagggttgaa
     1921 aacagataga gcaatatggt ggagtcgaaa gaggaaatcg tcttagaggg aggacatgtg
     1981 ggtcatcata caaccaaccg aaaaaaatca tgagtaggtt tctttttgta acaggtggcg
     2041 tggtctcgtc attagggaaa ggtttgtcat ctgcggccat cgggcattta ttagaaagcc
     2101 gtggcatgac tattaccttt ttgaagctag acccctatat taatgtggac ccgggtacca
     2161 tgaatcccta tcaacacggg gaagtctatg ttaccgatga tggagcagaa accgatttag
     2221 acttgggtca ttatgaacga tttaccacgg tccagttgca tcgagaaaat aattgtacga
     2281 caggacggat ttatgagtcg gtgattcaaa gggagcgaag aggagaatat ctcggagcga
     2341 ccgttcaggt cgtcccgcat attacggacg ccattaagca gacaattctt aatgtggccc
     2401 atgacgtgga tgtggtgatc gtggaaattg gcggaactgt cggagatatt gaaagtttgc
     2461 cgtttttaga ggcgattcgg caaatgcctt atgaggtcgg tagggaaaac gtcctgtata
     2521 ttcatctcac cctggtgccc tatattggaa ctgctggaga actcaaaacc aaacccactc
     2581 aacattccgt taataagctt cgtgaaatcg gcattcaacc caatatactg ttatgtcgga
     2641 ctgacagatt tctccctcct ggcgtcaaag ataaaattgc catgttttgt aacgtggata
     2701 aaggctctgt catcacagcc aaagatgtgg attccatcta cgaagtgccg attatcctga
     2761 gaaaagaagg attggatgaa cttattgtcc gttcactgca tcttgaaacg cggccaccca
     2821 accttcggga ttgggatatt ttagtccaga agattaagcg gccacgacat gaagttacca
     2881 ttgcactggt tggtaaatat gtagaatctc gggaatccta taaaagtgtg tcggaagcct
     2941 tgactcatgg cggattacaa gatgaaacag ccgttcatat tcattgggtg gaatcgggtg
     3001 acattgagaa ggacggcccc gaacgcctgc tagctgatgt ggacggcgtc ttaattccag
     3061 gagggtttgg gcttcgaggg atcgaaggga aaattgatac gatccggtat gccagagata
     3121 gaaatcttcc attctttggc atttgcttgg ggatgcaatg tgcggtgatt gaaattgcca
     3181 gaagtctagg tggacttcag aatgccaata gttcggaatt caatcctgat acgccatatc
     3241 cggtcattga cctacttcct gagcaacgat ccattcaaga gaagggcgga accatgcgat
     3301 tgggaggatt tccctgtcga ttaattccca atacccttac ctttgcggcc tacgagcaaa
     3361 cggagattcg tgaacgacac cgccatcgct atgaattcaa caatgagtac ctggaagctt
     3421 taacttcaaa gggtctgacg attagtggga catcacctga cggaaggctg gtagaaatca
     3481 ttgaattaca agggcacccc tggtttgtgg ggacacagtt ccatccggag tttcagtccc
     3541 gactatgtgc tcctcatcct ctttttacgg catttattgg cgccgcgctt caaaaaaaat
     3601 tcggtacata acgaacagga atgatcatgg ctcagccaac tcaagtcgac attggcccgt
     3661 tctccgttgg cggaagttcc ccccattttt taattgcagg gccttgcgtg atcgaaagcg
     3721 agaagttggt catcgagaca gctgcagcga ttgcggaaat tgccaaagat cttggtatcc
     3781 catacatatt taaggcgtcc tatgataagg ctaatcgcac atcggtttct tcattcaggg
     3841 ggttaggcat tacagaagga ttgaaaattc tcaagaaaat caattgtgaa ttagggatac
     3901 ccatcctcac ggatattcac gaagtccacg acgtttctca ggttgccgaa gtcgtggacg
     3961 tgttgcaaat tccggcattt ttatgtcgac agacagattt actctctgct gcggcaaaat
     4021 caggccgggt ggtcaatgta aaaaaagggc aattcctttc tccatgggat atggctaatg
     4081 ttgtccataa acttgaagac gccgggacca gaaaaatttt cctaacggag cgaggggcca
     4141 gcttcggcta tcaaaatctt gtcgtcgata tgcgctcatt acccgtcatg agaaatatgg
     4201 gatatccggt ggtatttgat gcaactcaca gcgtgcagtt gcctggtggc ggaggaacgg
     4261 tctcttcagg acaacgggaa tttgtggcgc cgttagccag agcggcagcc gctgctggat
     4321 gtgacggatt ctttatggaa gttcatcctc gtcctgagga agccctatcg gatgggccaa
     4381 atatgatacg gttaagtgaa ttgcgcggat tattggaaca aattcaagcg atttggcagg
     4441 taacaggaaa gggagaaatc tcgcctgtta cttgatattt cagcaataaa tatcagagtt
     4501 gccatgaatc aatcggaagc agtagtaaaa gaaaaatctg aacgaaataa tgtaattatg
     4561 ggagaagcct atctcaatct tcctaattct cttactcttt tgcggatttt attaattcct
     4621 gttttcgttt ggtttttttc tgaatccagc cctgaacgag ccttggcagc cggactggtg
     4681 tttgcgtgtg ccgctttcac agattttctt gacgggtatt tggctcgaaa aagcgggcaa
     4741 attaccaatt tagggaaatt gctggatcca gttgccgata aacttctggt ggcttcagga
     4801 cttattcttc ttgttcaatt tcagcgagta gctgtgtggt tggccatcgt catgattgcg
     4861 cgtgaaatga ttgtgacggg agccagggtg gtcgcagcaa aagaaggctt tgtggttcca
     4921 gccgactctc tggggaaatt caaagtgatc gggcaaatcg gaggaattct ttgccttatt
     4981 cttcagggag tctgggttca aagcgagggt ctactggcta cgattgggac cggactgctg
     5041 tatattgcct tgtttttctc ccttttttcc ggctggcgat atctcatgca aatcttcaag
     5101 aaaatcagtc cgcaatattg gtaggagtgc tcagaagtcg ataggagatg gaattctatt
     5161 tattaggagt ggttggcgcc tatctcttag gatctatccc atttggcctc gtcgcctctc
     5221 gtattttcgg aaaggaagat cctcgcactc gggggagtca taatatcgga ttcaccaata
     5281 ccctgcgggt atcagggaaa aaagtaggca tacttaccct aattggtgac ttagggaaag
     5341 gaacggtcgc gacggttgtc gcaggctcaa tggggtttcc ttggctatgg attcttttca
     5401 taggcttttc agtcattctt ggtcatgttt tttcaatttt tctacgattt aaaggtggaa
     5461 aaggagtagc cacagcctta ggggccatta tagggattca tccgttgatt ggatggcttc
     5521 ttgttggtat ttggttggga gcggtatggg gcttcggata ttcatcaggt ggagccctga
     5581 tagcatttgg tgcgttccca tttttggtct ttctcctgac ttctgacttc tatttttgca
     5641 ttttttcttt gggagtcatg gcaatcatat tcatcagtca caaagaaaac atacaccgtc
     5701 ttaggcaggg aaccgaaagt aaaattaacc taacttctac ttaacataat atttattatc
     5761 cgacgttatg aaatataagg tttaggatga aattaggtca cttttcactc gtattttcat
     5821 tatcttgcca tcttttgttt attgctccgt cactcgcaat tagccagaca gccaaagaac
     5881 ttcgggggct agaacttctt ggagatatcg aacaggccat caccagcctg gccgaacggg
     5941 tgactcctac ggtggtcaat attacaccaa tccatgagat agctcaggga caagggtttc
     6001 agcgacgtgc gccattctct aaaggcagtg gatcaggtgt catcgtgcgg gaagatggaa
     6061 tcatcgtgac caataatcat gttgttgggg aagacgccac ggaggcagat gtccggctat
     6121 ccgatatgtc caacatgatt gctcaggtca tcggacgcga caaggaaaca gacatagcgg
     6181 ttctcaaaat tcaagctgac cgaaaatttc ctgcagccca ttttggagat tccaatgcct
     6241 taaaggttgg gcaatgggtg ttggctgtgg gaaatccgat ggggttagat cgcacagtga
     6301 ccctaggggt tgtgagtggg attggaaggg aacgcctcaa tttatcccgg tatgaaaatt
     6361 tcattcaaac ggatgcggcg attaatcctg gcaattcagg aggaccgctc tttaaccttc
     6421 gaggtgaagt gatcgggatt aataccgcca taatccatat ggcacaagga atcggatttt
     6481 ccatacccgc cgacatggtt tcacgtgtgg tcgatcaatt agtgtcccag gggcgagtgg
     6541 tccgcggttg gcttggtgtc gggattcaat ctctcaccaa agaacttgcg gaacaatttg
     6601 ggattaagga aggccatggg gttctcgtca acgaagtcta tgaacacgat cccgcccatg
     6661 tggccggaat taaaccaggg gatatcatcg tgtccgtaga cgacagcccg gttgattcgc
     6721 ccaaccaact gtctagactt gtggcgcgag tcggacctgg tgagaacgcc aaaattcttg
     6781 tgttacggga cggcaaggaa atgacctacc tcgtaccgat ggccgaaagg caggaaaaat
     6841 ccatgttggc ctcactcccc ttacaaaaat cagaagtcac cttaggacta gatgtccagg
     6901 ggcttaccgc tgcacttgcc gagcaatttg aactacaaga aactgtgggt gtgctcgtga
     6961 ccaaagttga accgggtggc ctcgccaact ctgagggagt tcaagaagga gatttaatca
     7021 acgaggttaa tcggcaaacg gttcgaaccg tcacgcaatt ttctgaagcg gtggccaaag
     7081 taaagccggg tcaaacggtt ttacttcgaa ttatccgaaa gacaagggca tttttcattg
     7141 taatcaaaac gctgccatag cgtttctctc tgtttatcat caagcaatta gtgtgaggaa
     7201 acggcttctt tttctttttt atgttcttcc actattcgat ttaggcaatc cttcggaact
     7261 tcctcgtaac ccgaaaattc catcacataa gtgccctttc cggcagtcat cgacgttaag
     7321 gatggagcat atttcagcat ttccgccaga ggcacaaagg ccttcacgat ttgattatgt
     7381 cctttcgtcg ccattccctg gatgcgtccc cgccgtgagt taagatctcc aatcacggtt
     7441 cccaccaaat catccggaac taggacttct accgacatga ttggttcaag aagggtgctt
     7501 tgagcagaat ccagggcatg cttgagggcc attgatcctg ccactttaaa ggccatctcg
     7561 gaagaatcga cgggatgatg agatccgtca tagacagcca cacggatatc cacgatggga
     7621 aacccggcaa caataccatg ctgcagtgct tcgatcacgc ccttttccac tgcagggata
     7681 aaatttcgtg gaatgacacc accaacaatg ttgttgtgaa attcaaatcc ggtcccacgc
     7741 ggaagaggtt caatttgtag ccaacaatct ccatattgtc catgcccacc ggtttgcttt
     7801 ttatatttcc cttgcgcctg agccatccga tggatcgtct ccttataggg aactttcggc
     7861 atatgcaaat ccacttccac tccatacttg cgcctcagct tatctaaagt ggcctcaatg
     7921 tgaccctgcc ctaccccact caacaacatt tcttttgtct cttcattcct tagaaactca
     7981 agtgacggat cttcttcgac taacttatga agacctaaac tgaccttgtc aatttcattc
     8041 ttgcttttca cttctaaggc ataagacatg accgggcgtg atattttcag gcatgggaat
     8101 atgatggaat gtcgttcatc acaaatggtg tcaccggttt gagtgtcctt taatttaccg
     8161 attgctacaa tatctcccgc agaaacttga tgaacctggt gatgtttctt gccaatggaa
     8221 aaaaagagat gcccaccctt ctccctggtc tgccgggagg cattataaaa ccctgaatct
     8281 gcctgtaagg ttccggacag cactcgcaca aatgaaagac ggcccataaa aggatcgata
     8341 gtggttttga aaacgaaggc tgaaaacgga tcatatggat cagattttcg ttgaccttca
     8401 tcatgggtct ggggttgctg cccgacgtgt ggatggagac tggaacggtc ggaaggggat
     8461 gggagaaacc gttgtactgc agccagcaag gatgttgttc caatattgtg aatagccgat
     8521 ccgcacaaca ccggaacaag cttccgttct aatgtgccga tcgtcagacc atcacgcaaa
     8581 tcttcgtctg agatttcacc ctgggagaga tatttctcca ccaattgatc attcgtttct
     8641 gccacctgtt ccatggcacg gcgccggaat tcattcgcaa tgggttgatg ttctgcaaca
     8701 atctcggttt gctggatttt tgtcgtgccg gaaagcggag tgatgacatt cctcgaaatg
     8761 agatcaatca ccccggtcgg catggccccc tctccaaccg ggatcgtgat tgggactccg
     8821 gtgaatccca atgttttctc acactccgcc aaaatcgagc gatagtccgc tccctcttta
     8881 tccagctcat taataaacag gagacaggga gtttcgtgtt cctgaatgag atcccagact
     8941 cgttcgattt cggaccgaag tccggtactg gctcctacaa caagcaccgc accatccacc
     9001 gccctgaggg cggattgaat ctcaaaggca taatcgctca ttcccggtgt atcaataata
     9061 ttgatttttg tcccctgcca ctcgaactga cagatagccg aactgatgga atggtgtctg
     9121 tggacttcct cgggttcaaa atcaccaatg gtgtttccct gggaaatggt ccccatggct
     9181 gggatgactc cagtggaaaa ggccagtgct tcacagaggg tggtctttcc taccccaggg
     9241 taggaagtaa tgaccacgtt gcggatggat ggggcggatt cgtgtggcat gcgaacctcc
     9301 tcttaatttt cttgaattct aggtaggaga ggaaagagtg tcaatggaaa aagcgaagtg
     9361 ggctgtcagg agatcaacgc tctttttatt ttatcgtcag tggatcgaca gataaaagcc
     9421 aagaaaatat gcgccgtggc tctaaaaaaa aaccattcag gcaaaatttt cctagtaaag
     9481 tccaaggttt tctcataaaa atggttcttc gtcttttgtc agaaaattct ggcattcagg
     9541 ttgctctagt gtcatatcta agagagttga attgatacca actccatggt ggaccatcca
     9601 cccgtatcaa tgaccgaaac gatgtaagtc gttccatctc tcacccctct tctatcacag
     9661 gctgatgccg acgtgaggat cttttgagat ccaaggaaag ctttgcgtcg aaatctaggt
     9721 attaacactt aaattcaagg gagggatcgc atgagcacat tgacaccaga gttggcgggt
     9781 atgacttcaa agaatccatt tgctgggtta cagcagctca atgtggaggt atttcctgct
     9841 gacttttctg atgttcgtgg acattcacca cttcccttct ctttgcggtg cctaggaggg
     9901 acttgggagg ggtacctgat tcaatgggac gagcacaatg tacgattgca tttgactgga
     9961 tttcctccaa ttgagctatc gcagatcatt gagtgggaat atcgtatttc ggatcaggac
    10021 cattccctga ccacaatcca gggaatgatc aaagatatcc atgtggaaac gggtcatacc
    10081 gcctgggaag gaacaacaaa cattttttta cattgttcaa cttcgacggg tattagattc
    10141 cttccaaatc aaggagaggg actcgtggcg tatgggcacg ttaagccaga aggctcggat
    10201 tcagtttctg gttttgatcc tcgtgagcag gagaaagagt tcaaggccga gtccattcat
    10261 cccccaaaaa agggcgtgcg cagtaatcgt ctcaccattg aaaaaagcaa tgggcagagg
    10321 attcgtgcct tccatgaccg accaattgaa ggaaatccaa cagaaggacc tgtggtcgtt
    10381 attgcccctg gatacgggga aacgaagcgg gattatctga ccttggcata ttactttgcc
    10441 agcaatggat ttcatgtcgt acgctatgac cataccaatc atatcggcga aagtgatggg
    10501 ctgcaccatc aaatatgtct ttcatcaatg aaggatgatt ttcaaaccgt gacccgttac
    10561 gtcaggacaa attggccggc ctcagcaata atcggtgtgg cctcaagttt agcgtcacga
    10621 gtcgccctca aagccgaggt tgaatgtcca tcagtatccc tcttgatcat gctcatgggc
    10681 attgtcaatg tcgaacgctc cgctgccacg gttcatcagg aagatctctt cgccggctat
    10741 ggtaagggac agtgccctga gtcagcgaat tttttgggtt ttaatgtagg aaatcatttt
    10801 ttgggggatg ctattgcgaa ccaatttgtc acgcttgaac acaccctgac tgatgcggca
    10861 tcggcaacct ctcccgtcat actggtttcg gccgaaaaag atgcctgggt ggcctccgat
    10921 gatctacagg cgtttcgaaa cgcattgggt cgacgtcttg aagaatggat tgtggtgcct
    10981 aatgcattgc atcgcctgca ggaaaacccc aaaatagcac gtgaaactta tcgacaactc
    11041 atcgcacagt gccaaaacca tatcgcacag accccttctt cggagacgat ccacaatccc
    11101 aatcgactag acttgggacg tcaaaatcga cgagaaaaaa ttgcgcaaca acaacaggca
    11161 gagaccgacg tcggggcagg cttttggcag gattacttgg gccattttca gtcggttgcc
    11221 aaatgccggg attatgtgaa actcctcgac catgcgtttc atgccttggg ccccattact
    11281 cctggccaaa gaattctgga tgccgggtgt ggaaatggca atgcggggtt gtattttgtg
    11341 aatgggctag cctccaccat ttctcctgga atctcgtgcc tggaggagtc cattcagtat
    11401 gtgggaattg atgtcgttcc tgacgcattg aagcgcgccc atgaagcgat ggtcgcagcc
    11461 accaccgaat ggcaacatac caagatctct tcaacacctt ctgtcacaaa atcatgggca
    11521 caagtcgatt taggtcagcc gctccccttt cccgacaacc aatttgaccg aattgtctct
    11581 aatttagtga ttggatatgt tcaggatcca ggtgcggctt tgcgggaact atatcgaatc
    11641 ctggctccag gcggacgaat ggtcatatcc aatttaaaac cgaatgggga tttttccggg
    11701 atttaccaga gcctggtcag caatgcggcc ctcccacagc aaagggagga agcacgggat
    11761 ctcttgaata attacggaaa aatcagacag gccgaaaagg aagggcaatt ttgttttttt
    11821 gatcgaacac aatgggaatg gatcatcaat actttgggcg gtgcaaatgc ggggattttt
    11881 tcaacgtttg caggacaagc ctatctcatc gtattagaga aaccagtcgt ctgcgcccaa
    11941 gcgcaaagac caatgatcct gaataaaaaa caatccccaa gactaacacc cctgccggga
    12001 gccttgaaac aagtggcata aggcttcccc gttctgcaaa tatatttcag gagtaccaca
    12061 tcgccgcagg cgacgcatca atgacattga tcacgcatga aggggaaagg accgtgccat
    12121 tcacggaggc acaagaggag atgccctccg gcatcaaatt gccaactctt ttgacttctg
    12181 gatgcgttta ttgtccaata gcctttctaa atgatgtaca tagccaatga tctagtttaa
    12241 tcaactatct ttcattgttt ttgacgtgaa tttttacgcc ttcagcggac tcctgaatgg
    12301 tctcgccgcc acggcctccg gccttattgt ttacgctaaa aaccctcgaa gtcccaaaca
    12361 tcaagcctat gccctgtatt gtttagctgc cgccatttgg ggatatggtt tttgcgcctg
    12421 gcagcttggg tcctcccacg atctcgccct tttctttgtt cgcttgctga tggtcggagc
    12481 catctttctt cccgcagcct atctgtggca cgtcctaacc ttattggatg atgtggaatc
    12541 gaacagatgg attatccgtc ttggatgggg aaccagtttc atttatttct tcgcaaattt
    12601 cacctcctat tttgtggccg atgttcatcc tgttggtgat tttctctttt ggccgaagcc
    12661 cgggcctttt tttcatttct atctactggg gtttggccta tcaactattt atggaacatg
    12721 gttgctctac catggagcac ggagcagaac tggtcctgaa cgttcgcggt tttaccttct
    12781 caccatcgga tcagtcattg cgtatgcagg gggagtgacg acttttcctc tttggtatgg
    12841 gattgagatc cctccgaacg ggacaattct tctgactgtc tatacgtcgg tcgtagccta
    12901 tacactcctg cgataccggc tcatggatct cgggacggct gttgaacgag gcattacggg
    12961 aagccttctg ttagccctcg tagccttccc tgcttatccg atcctccttc ttgggcaaag
    13021 tctgtacttt ggggaaatta atatcgccta ttcttttgtg gaattgagcg tattgcttat
    13081 cttggtgctt ggagcgtctt ctttgaaacg ggaagccaaa acattaatca cgaaatcatt
    13141 gttcaaagaa cgatatgacc gtcatgaaac ggtcgttcat ttctctaaat cactcgtctc
    13201 cattctggaa ttaaaagatt tgacggcaac catcgtggaa tccatttgtc ccacattagg
    13261 attggaatgg ggagtcttat atctacgaag ttccagcagt caggattatc gaccggtatc
    13321 cagctttggg aaatccacca atgatcttcc tgtcctctat ttgcaaaaaa ctcctacttt
    13381 gttagaaatg tggagacaag gcacgtcctg ttttgtcatc aatgaattgg aactctctac
    13441 catcaactct gactctgaag ccgtcatcgc gcaactcgcc gacattggaa cagaagtttg
    13501 tttgcccttg gttaacaaaa cacggctttt agggttttgt gtgtttgggc ccaatacgaa
    13561 aagtggcggc gggtatacga cccaggattt ggatttattg acgaccctat cccaggaagc
    13621 ttccgtagcg ttagacaatg ccttgctgta tgaagagctc aagcgatcac agtcgttagt
    13681 tcgtcggact gatcggcttc gttctctgga gaccatggcg ggcggattag ctcacgaaat
    13741 tagaaatcca ctgacgtcga tcaaagcatt tgtggatctg gcacctgaac gaaaggatga
    13801 tgagcagttt ctcgtacgat tcagcaaggt agtcaaagaa gatgtgttcc gaattgaacg
    13861 gctcacgcgg gagatcttgg attatgccaa accgatggaa ccgtttctca aggaagaaga
    13921 ccttaatgat attgtggaat cctgtttgta taccctcagg attcgtcctt ctcacgagtt
    13981 gattgttgtc gaaacagatt tagcccatgg gttgccaaag gtatttgccg accggcaaca
    14041 attaaagcag gtgtttctta acctattgtt taatgccgtg gaagccatgc ttccagaggg
    14101 tggcgtgttg acagtgcgga ctcgaataat tggctcaggt ctggctcaga actgggtgca
    14161 ggtggacatt caagataccg gaaaagggat taatccggaa gacatcgagc atatttttga
    14221 tccttttttt accacaaaac acttgagtca ggaacatgag ggaacaggct tgggtttggc
    14281 cattgcccat caaattattc aagaacatcg tggaagtatt gaggtacaaa gtgtgagagg
    14341 agggggagcc acttttcttg tgaacctccc aagctcacca gcaccgacat cagtttctcc
    14401 aacgcagagt gtgttgccca atccggaaca ccgataacat ggattgaaac accggatgaa
    14461 ggatccgatc aatgtatgca gtcatagggt ggagggttga cttgtatagc cacgcgagga
    14521 acggaatctc tccacaaggc atactcagca agctttatga ccgttgagta cggggtaagg
    14581 aacaataaat tcacgttaac cagtgcgcgg aagtcgccct tcaatcaatt gcccatccca
    14641 atctccggtt ccctctttca gatggagaac ccgagaacga gtgagtccgg ctcgttgaag
    14701 ccactctcga acatctcgga cggaataggt attgccctgc tcagtataaa gtaacatgga
    14761 gacggcaaag agatttgctt caagaggccg aagttctttt tggtcctcca aaaatgtgtc
    14821 ttggatgaga agccgtccgc cagggttgag aattcgtttg atttttttaa aaattttcag
    14881 gttgtctgcc ggagaataga tatgaagaac attggaatac cagacgacat cataggtgcc
    14941 ggaaatccgt gcagttaaaa aatcaccagc ttgacaggtc aaacgatttc ccaaggagga
    15001 ctgttccgcc agcatacgtg ccacatccag agcagcaggc cgatccatca ctgtggcgtg
    15061 aaggattgga ttcagcgcta aaaacgccaa agcataagtc ccgggcccgc cgcctaagtc
    15121 gagaagggaa cgagccgctt ttagagaaat ctgttgtgcg acctctctgg ccggttggag
    15181 ggagcgatga tgcatggcac aggaaaacga ccgtcgatat tccgtggttt ccggttcctt
    15241 gctgtcccat ggctgaccga cacgtatgac ttctgtcaga tgggaccatt cgctccattg
    15301 acgctgcata agtcccagat agtctccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnntgt aatcgctggg tattttcggt gagttttggt aattgatatt tttggtctat
    15481 acgaacgact ttttgtaagt attgcacggc ttggttccat tggccttggg cctcatggca
    15541 actggcaaga acataccagg caccgcccat acggagttca tccttcagcc gcctggcgac
    15601 atctagactg gtcaaggtgc aggattgcgc ttcctcaaat cgtcgttgac tcaaatataa
    15661 ccgcgccatc atacgaaaga catcggattg cccggcctca tttccgaccc gacgcatcag
    15721 gattaatgat tcttgatagt aggcgagcgc ttcttcttcc ttgccggttt ccctcgcaac
    15781 taaccccaaa tcagaaagta aaactgcctt tccggcatag tcggacaact gattcattaa
    15841 atccaaggcc tctaaataat aggcctgggc tcgttcccat tctccagcat cggccgaaag
    15901 attccccaaa ttggctaaag tcgtactaat tccccgatca tcaccaagaa ttttttgaag
    15961 ttctaacacc tcactataat acgctctggc ttgttcccgg cgaccactca ctgcgcaaat
    16021 attaccaaga ttgcccagtg tggtggccaa agatcgttgg tctccatttt gacgatcaac
    16081 ctccagggcc tgcgcataat attgaaaagc ttcggtatag aagcctttcg aaaagtgctg
    16141 attgcctaaa gaattgagat tggcggattg agaccaggcc atacatagtc actcggcgtc
    16201 acaggacaga aaaaatcttc atgccattct ataatattga ttgattcaat tatctaccaa
    16261 gaggtcttca agtgcgggct ttgccccagt gagaatggaa gtgccatctc caacaaggag
    16321 actgtcaaac gtatatttta ataacctacg aagaccttca cgggctagga ggatgttctc
    16381 atatttctct ggaggtaata gccgtagtgc acctgctgga tccccaatga ttccatctcc
    16441 aacaataagc acacccttgc cttgctgcaa gaataaggcg ctttcccctg gagatttttg
    16501 atcggaaagt tgaacaaccc aaattccccc tggaagcaat tcaccgtctt tgaaggtttt
    16561 atcaatgggc agcgtcattt ctttggcatc caattccgga gccatcacct gacatttaaa
    16621 ggttttcctg aagttggccg cttcccgttc atgatcccga ttagtcaata taatataatc
    16681 cggagaccct ccccgctgcg tcatggccat atcacccgcg ctcatggatg gggggtcgac
    16741 tagtatccga tgttccccca cggctaataa atgtccgtta aaattaatct gtttttcttc
    16801 acagaaccag gaccattcag aaatattggg tagaatggtt ttcataaggt ttccagggtt
    16861 tcttgaagaa gagttgtgat tttggtttga acgccagatt cttctggaag gtccaacata
    16921 tcatcctcat ggattgtaat aaatttacga ttggggccct tcgtaaggga aatgagtaaa
    16981 agactgcgcg aaggagtggt cgggatgact acttccacag aggaatccag agtattcact
    17041 atctcaagaa atttttgttt tccgtcttct aattcgtcca tagtgcccta acatcggttc
    17101 aagaatgcgt aaaaatagtc ctcaacctac atggtcgagg gagtagacgg tgactccaat
    17161 aatttctcca cttcctgcag aatcgcttca tgtccggcta atgtgccgtc ggtaaagaaa
    17221 ttccctacgc gctgccaacg gatgaggcca tgttgatcaa taacatacac gttgggaata
    17281 aattttcccc cgccatagag tttgtctgtt tttttctcgg ggtctaggag atagggatac
    17341 gtcaccttca ccgggaatcc ttgtaagaat tccctgacat cggccatgga gtctccagag
    17401 gaattgactc cgatcaccgc tacaggcttg tcttttatgg cctcatgcac catctgcaaa
    17461 ttggtgcttt ggattaaaca cggctcgcaa atatgaaaga gtcctagaac cacgaccttc
    17521 cccttaaact ggtcaagaga tagagtttgt ccatccagac ccgtcagtga gaaatttgga
    17581 gcggactcac cgactttaaa aaaacttccc gcgtgtgaga tgaaactggt acccagtatt
    17641 aatatggcta caatcagtac ctgatgaata ttttttttca taggttcctc ctgttcatgg
    17701 tgcctcataa ggcatataac gagagattca atcttcccat ccctgaaagc ctaccacatg
    17761 aatttttggg gcggcaacaa aacagaaaga catttaggag tgagagagtt gatctggttc
    17821 gaataagccg agttgaagtt gttcggcttt tgtcaacatg atgggataat tatccgtaaa
    17881 acaggcattg caataatgat tagaaaagcc gggagccatt gctaacatgc cttctagact
    17941 aaggtagcct aagctatcag ccgtgacata acgacgaatt tcttcaatat tgagatttgc
    18001 tccgatgagt tccttttgtg tcggcgtatc aatgccataa aaacatgggg caatgactgg
    18061 gggtgaactg atgcgcaggt gaatttctcg agcaccagcc tgccgaagca ttttgacaat
    18121 ttttcggctg gtggtgcctc gaactaggga gtcatccacg acgactatcc gttttccttc
    18181 taagacatcg ggaacgggat ttaattttag ccttacccca aaatgacgaa tggcttgttc
    18241 cggctcaatg aaggtccgtc ccacgtagtg attcctggtc agtccgatct caaacggtaa
    18301 ccccatccct tcggcatagc ccaaggccgc cggaacaccg gaatcgggaa ccggaataac
    18361 catgtctgcg tccgctggac attccttggc caattgacgt ccaaaggctt tgcgaacggg
    18421 gtacacggca tgagggccga aaattttcga atcgggtctg gcaaaataga cgtattcaaa
    18481 tacacattgg gcatgcggtc gttccagaaa cggatgatat gaggtcattt ctgaatcgtg
    18541 aatgacgacg atttctcctg gttcaacttc acgaacgaat ttggcatcaa tcagatcgaa
    18601 tgcgcaggtc tcggaggcca caacccaact gcccttataa cgccctaaac agagcgggcg
    18661 aaaaccaaac ggatcgcggg ctgcaattaa atgattatca gtcaatagca ccaaagaaaa
    18721 ggctcctcgg actaggctta gtgcgtcaat cacccgattg acgatggtat ccccttttga
    18781 gtgggcgatt aaatggatga tgacctcgct atcggaatcc gattgaaaaa tagcgccata
    18841 ggcctcgagt tcaccacgaa gcacacccgc gttaattaaa ttcccattgt gggctaacgc
    18901 caaatttcca aatgcaaaat taacggttaa gggttgaaca ttttgcaact gtccactccc
    18961 agccgtggaa tatcgattgt ggccgatcgc tttggtgcct gaaagttcgc gtaatgtttt
    19021 ctttgaataa atatcggcaa cgaggcctag gccttttttt tgaaagaatt gctctccatc
    19081 cgaagacaca attccggagc cttcctgccc tcggtgttgt aaggcataga ggccaaggta
    19141 ggtcagattg gcggcttcct tcgaccccgc aatcccgaag acagcacatt cttcatgaaa
    19201 cccgtctgaa gatggcatgt ccgacaccat gggcaacttt ctcatgagat cctcacatag
    19261 aacagatcta cctgatcaac aagaagggcc gaaaatgtcc aatacatcca gggccaaact
    19321 attatcacga ttcttctgtt cctaattggc gtgcgagact gtggtgccat gcacttccca
    19381 tttcggaaag ggacaggctt atttgagaca cgggctggtc attgcctcca cggacagtga
    19441 tgtccatgtt ttcctgagtg acctgtccca attctatgct cgggactccg aagccttcaa
    19501 ccaactctct tactcgctta atattctcgg gttttatgga aatgacaacc cgtgaaggac
    19561 tttctccaaa aagcagggcg tctagacgca ggcgatcttg agtcagtata acagttgccc
    19621 cgaatggggc ggaaggatga gtcagacagc actcggctaa ggtcacgagt aatccacctt
    19681 ccgagcaatc gtgcgctgat tgcaccagcc catcctgtat caatgaaaga aggcaggagt
    19741 ggagggcctt ttcccggtcc atatccaacc aggggggatt gccttgttca cgtgaatgga
    19801 taaccttaag gtattctgtc ccgcccaagt cctcctttgt ctctcccagc aggagaacct
    19861 gatcgcctga ttgcttgaac cattgagtga taatgttttc cgggcgctca atgagtccga
    19921 ccattcctac aatcggggtg ggatagatag agagtccatt cgtttcgtta taaaaactga
    19981 cgttcccgct cacaacagga atttcgaatg ttcgacaggc gtcggcgatc ccttcgacgg
    20041 ccatgataaa ttgccacatg atgtcaggac gctcaggatt gccgaaattc agacagtcgg
    20101 tgaccccgat cggtagggcg cccgcacaga ctaagttccg cgcggcttca gcaaccgcta
    20161 atcctcctcc caaataggga ttcagcagac aaaagcgact attgccgtcc gtcgtcatgg
    20221 ccagggcttt ggaggttcct tttacgcgaa ctacggcgga atcggatcca gggcacacca
    20281 cggtattagt tcctaccata tgatcatatt gccgaaacac ccaacgtttg ctggcgatgg
    20341 ttggtgaagc taagagtttc agtaatacct cagcagggtc tttaacgtca ggaagagcgt
    20401 caatatttaa ggactgcaaa aactcttgat actgaggtgg tacggcagga cgttcatagc
    20461 gtggaccttc ttctgcgagg gcatgagcgg gtatttcagc cacaatctta tggccttctc
    20521 tgattcgaag gaatccatca tccgtcacac gtccgaccac tgcggcatca atgccccatt
    20581 tccggcagat ggcaagaact gcctcttcct tgccaggttg agccaccagc aacatcctct
    20641 cctgagattc cgataacaga atctcatagg gagtcatgtg cggttcacgg cgagggactt
    20701 taactaagtc catgtcaata ccggttccgg cgcgggaggc catttcgcag gacgaactgg
    20761 tgagcccagc agcccccatg tcttgaatcc cgaccaacag gtcctgttcc aaaaattcaa
    20821 ggcacgcttc aagtaatagt ttttctagaa atggatctcc aacttgtaca ttcggacgtt
    20881 ttttttctga ggcatcatca aaactatcag aggccatggt ggcgccatga atcccgtccc
    20941 gtcctgtttt ggcaccaatg taaagcactt gatttccggc acctttggct aagccccgca
    21001 ggagacgatc tttttttacg atgcccagac aaaagacatt caccagtgga ttttttgaat
    21061 aaatatcgtt aaatgtaatt tccccgccta ccgtgggcac ccccatgcag ttcccatagg
    21121 aagaaattcc ggaaacgact cctttgagga tatgccggtt ctttggcagg tccaattcac
    21181 cgaatcgcag ggaattcaat aacgcaatcg ggcgtgctcc catggtaaag atatcgcgta
    21241 gaatcccccc gacaccggtg gccgcccctt gaaacggttc aatatatgac ggatgattgt
    21301 gcgattccat cttgaagact gccgcaagtc cctctccaat atccacggcg ccagcgtttt
    21361 ccccaggccc ttgaaccaca cgctccccct cggtcggaaa tcgtttcaaa tgaatgcgtg
    21421 aactcttata actgcagtgt tcactccaca ttacagaaaa gatgcccaac tcagtgagat
    21481 tcggctcacg acccaggtgc ttgaggattt gctgatactc atcctcggac agaccatgct
    21541 gttcaataat ttttgaggta agagagatgt gcggcgaggc ctttgagtcc tgtgtcataa
    21601 caagtcaatt atcctgaggt ttgcttttct caataggtaa acagcggagg agaaaggtca
    21661 aaaaccaaat atgatactaa aagtttagaa acaaaccgat gaggttcatt tcctcaacgt
    21721 tttcagatat gtaaatggga aagctaggaa agccgttatt ttgatgaaag gtaatttagc
    21781 ataaatacaa tcatactgtc ctgtgggtta atgccctgaa ggaaggcgaa ggggtaaatg
    21841 gtccgtcaca tacgaagcat atcgcaatgc cgtgaggatc tgttcatatg tcagattcaa
    21901 cgcttcttga acatcatcga atgattttcc aatgccaagg gttccgacaa tggtggccac
    21961 gtcaacgacg gtgcctgcca cacatggttt cccatggcaa atgtctgagg tcatcgtaat
    22021 tccgggaaaa acttccatgg tggtcttaac
//
LOCUS       DAORAQ010000035        20647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_469_length_20647_cov_67.502331, whole genome shotgun sequence.
ACCESSION   DAORAQ010000035 DAORAQ010000000
VERSION     DAORAQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_20647_cov_67.502331"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKM72_05255"
     CDS             <1..677
                     /locus_tag="PKM72_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNP60225.1"
                     /translation="SKLLEVMTEPMPESSQHLLGSETYPALLAFAATKSALAFGPGMG
                     ISPETTEVLLQLLPQLKAPCVLDADALNGLAQHCQIFSSMKQPPILTPHPGEMARLLG
                     ASSPKQINEDRIGVSREFAMQHQVILVLKGARTIIAEPRGQVAICPTGNPGMASAGMG
                     DVLTGIITGLLAQGLSGWDAARAGVYLHGLAGDLAAATIGEPGLIAGDVLTAIPHALT
                     HTLSHS"
     gene            747..1211
                     /gene="tsaE"
                     /locus_tag="PKM72_05260"
     CDS             747..1211
                     /gene="tsaE"
                     /locus_tag="PKM72_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNP60226.1"
                     /translation="MTSAQETIKFGERLGKLLTGGDVLALTGDLGAGKTVLTCGIALG
                     LGIPMDQVSSPTFTLIQEYSGAIPLIHVDLYRLDGPSDISTLGLEEYFTPNTIVLIEW
                     AERFPQILPADHITICLEYGEAEDVRLFTVSGSGPRSIRIGANLQENLPKPH"
     gene            1384..4035
                     /gene="mutS"
                     /locus_tag="PKM72_05265"
     CDS             1384..4035
                     /gene="mutS"
                     /locus_tag="PKM72_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNP60227.1"
                     /translation="MKDQELSPLMRQFQDVKAQHSDAIVFFRVGDFYEMFFEDAEEAS
                     KLLGIVLTARGKAKGEGIPLCGVPYHTSTGYIAKLLKAGKIVALCEQVEDPKLAKGLV
                     RREVVRVYTPGTLYDHELLPSREANFLSALCYTPLSNQGNTTLGNRFGLASMDLSTGE
                     FWISESLMKHSLQEIVDELVRLEPKEVIFPAPLQEEMTSAFQSLPIARIVPRDATGFD
                     LESCRTILTRTFQVNQVEDLQLHGLRSGLQAAGGLLQYLAETQPTLAHSHIRRPLIRI
                     LEHEMQLDQATLRNLEVLKPVSEQRQSPTLLTTLDKTKTPMGTRLLRQWIVRPLTQVP
                     AIHLRQEAVKELVLNLGVRMSIRDHLKTVQDLERLNSRIVLEVANPRDLINLHRSLED
                     LVVLQQLLPSFQSPMLKNVHEAWDPLLDVSSWIADTIIPNPPLSVKEGGIIQAGVNGE
                     LDELRVLAKEGTRLLTEMETRERTRTGIDSLKVKYNQVFGYYIEVTKANLSRVPGDYH
                     RKQTLVNAERFTTGELQDLEGRLSSADQKMKSLENQLFGQIRLRVASATTRIQGMAEQ
                     LATLDVLTGLAEAASINRYHQPTVHEGGTIQITDGRHPVIEHIRQSEGFIPNDTTLDL
                     DTNRLLLITGPNMAGKSTYLRQVALIVLMAQMGSFVPAESARIGIVDRIFTRVGAADD
                     LSAGQSTFMVEMSETARILDTATSRSLLLLDEVGRGTSTYDGLSIAWALAEYILDRGH
                     LGARTLFATHYHEMTQLETIREGIKNYTVLVQEKGQDVLFLRKIIQGKADRSYGIQVA
                     KLAGIPKPVLDRARNILAQLEQDSSSNNVSLSDESVVDTPPLEQVSPKPHVILDEVKQ
                     MDLFSMTPLEALNRLADFKARLNEHNP"
     gene            complement(4139..4819)
                     /locus_tag="PKM72_05270"
     CDS             complement(4139..4819)
                     /locus_tag="PKM72_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60228.1"
                     /translation="MKGAKKKGLALVGAVALVGAMASSAMAIEAFQERFEWGDLSKPT
                     TLQGRVAVVDPYDKAVWINVAVFGGNAESGYYWQKYYPGKNLKVYPGSDEIWKTLVAA
                     GKDSSSQAVMTDTKPSNYGLVEIVVQETEQNHRVVSSAKSLQEVAGTPDKPRNISTLR
                     TTPVLDAIRADSWPDQRKMLYDVLIPDGKAVVGLIPYSDQGTGQIYDPAVASFEKVEV
                     HKEMGGKH"
     gene            complement(4879..5418)
                     /locus_tag="PKM72_05275"
     CDS             complement(4879..5418)
                     /locus_tag="PKM72_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60229.1"
                     /translation="MKKSSLRHELVALCAMGLLGLGLASIGYAAGPGPDKSKGDPNDV
                     TSQGDLSFKGEAVTSLKGRMYAQWADNPEGEILFGIQYWNASGPQETADPPGRATDDR
                     NVALPDPLYICCAWGYTGGNEKNNPYGGWYNPQTTVRLAVKDKALMDQLTKAAQELVA
                     MEVQLSGRTITGFKLLEAE"
     gene            complement(5604..6872)
                     /locus_tag="PKM72_05280"
     CDS             complement(5604..6872)
                     /locus_tag="PKM72_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNP60230.1"
                     /translation="MYKKVILDNGVRVVLERMSSLKSVALGVWATVGSRDEGKGEGGL
                     SHFIEHMMFKGTPKRTASQISNEIDALGGEMNAFTTHEATAFYVKVLDQQMGLAFDLI
                     ADLFHHSRFSAKDIAKEKQIVLEEIRTVQDDPEDYIHELHAKDIFGSHPLGRPILGEP
                     AVMKRLDRQLLMKYRQQHYRPEHTIVAVAGNFSFPEIIDTVNKYFGQWKRSKFDKHPS
                     TIQETPWPDQPQERQSLHVKPLEQVHVCVGFKGLPIGHPDRYAGHVLSTILGGGVSSR
                     LFQEIREKRGLAYTIYSHLSGFLDGGTLTVYAATRPNEVTAVIDRICQETKKLCRRDV
                     VSKELERTKTQLKGGLMLGLEGTYGRMNKLAKDEMYQGRHVTLQEMVKAIDRITPDQI
                     RHLSQKLFDLQQFVVTALGPIPKRGIPRWG"
     gene            complement(6893..8998)
                     /gene="pnp"
                     /locus_tag="PKM72_05285"
     CDS             complement(6893..8998)
                     /gene="pnp"
                     /locus_tag="PKM72_05285"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNP60231.1"
                     /translation="MIHVVEMEVGGRPLRLETGRMAKQADGAVLATYADTVVLATAVA
                     SKTSKPDADFLPLTVNYQEKAYSAGKIPGGFFKREGAPSEKEVLTSRLIDRPIRPLMP
                     EGYFFETQIIVSVLSIDQTMSSDVIGIVAASAAMAISDIPASEPLAGVRIGRVKGQFV
                     VNPDKKAQEESELNLVVAGTKDAVMMVEAGADGLSEDTMIDAIELAHREIKNIVAKIE
                     ELRAKAGKVKRVLQVEAIDPEIEQAVRKIAAAPIGEAMYIAAKADRQDRFAQILQEAI
                     EAVAGDDADRKAKVKKVFANLEYSEVRRIILETKKRADGRGLSDIRPISSEAGILPRT
                     HGSALFTRGETQALAVVTLGTSDDEQRIDALEGEYFRSFMLHYNFPPFSVGEAKMLRG
                     PGRREVGHGKLAQRALEAVLPSKEEFPYTIRLVSDILESNGSSSMATICGGALAMMDA
                     GVPIKTPVAGIAMGLILEQGGVAILSDILGMEDHLGDMDFKVAGTRDGVTALQMDIKI
                     AGITPDLMRQALAQAREGRLHILGKMDECLTVPRTELAAYAPRITTIQIKQDKIRDVI
                     GPGGKMIRSIIADCGVKVNVDDSGVVTIAAVDGESAQKAMDMVQRIVEEAEIGKIYLG
                     TVRKIVDFGAFVEILPGMDGLVHISQLAPHRVASVTDEISEGEQILVKVLEIDRQGKI
                     RLSRKDAMAEQTDKDQLTS"
     gene            complement(9125..9394)
                     /gene="rpsO"
                     /locus_tag="PKM72_05290"
     CDS             complement(9125..9394)
                     /gene="rpsO"
                     /locus_tag="PKM72_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNP60232.1"
                     /translation="MGLTKEAKTAAIESHRIHDKDTGSSEVQISVLTNRITSLTEHFK
                     THKKDHHSRRGLLRMVSKRRKLLDYLRRRDEGKYQAVIAALGIRK"
     gene            complement(9495..10484)
                     /gene="truB"
                     /locus_tag="PKM72_05295"
     CDS             complement(9495..10484)
                     /gene="truB"
                     /locus_tag="PKM72_05295"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNP60233.1"
                     /translation="MAPMASCIDQSKGLKAPQGMALHGVLNVNKPDGWTSHDVVQRLR
                     SGLGIPKVGHAGTLDPHATGVLPVLIGKGTKIAQYLLGWEKEYGAVLQLGQRTDTQDA
                     WGTVLEEFPIESLTEDRIRSAFATFQGSIQQVPPMYSAVKIGGQPLYKKARKGETVDR
                     PAKTVVIHDLEIQRLNIPEVAFRVVCSKGTYVRTLCDDIGNVLQVGGHLKWLQRRRVG
                     PIHVDQALEVESLLGGLEFSHLDGAFLSLDQALEGFHAVEVDSDDARKVLHGNAISWD
                     RVVHTFVESGVSPKEGNMVRVKQKDGQLLALGRGPVFQTGKGGPVLAIETVFS"
     gene            complement(10484..10903)
                     /gene="rbfA"
                     /locus_tag="PKM72_05300"
     CDS             complement(10484..10903)
                     /gene="rbfA"
                     /locus_tag="PKM72_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNP60234.1"
                     /translation="MAKTNSSRATRVADQIRMEVAEILSRKTKDPRIQFVTVTGVKMT
                     ADLKIARIYVTALDQAHFDQVTGPGLKSAVGFVRTELGRRLNLRYTPELVFYRDTSAE
                     YGNRIEKLLDSLHEESDEAGKAEASSFLHDKSESIDG"
     gene            complement(10928..11215)
                     /locus_tag="PKM72_05305"
     CDS             complement(10928..11215)
                     /locus_tag="PKM72_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNP60235.1"
                     /translation="MGMIVGLCTLELHIPDVQSLKGKRQVLSSLITRLRNRFNISVAE
                     IEEQDIWQKAILGVACVANDTGRVNQVLDQVLNFIRSNPSIELLRSQIEVL"
     gene            complement(11241..13850)
                     /gene="infB"
                     /locus_tag="PKM72_05310"
     CDS             complement(11241..13850)
                     /gene="infB"
                     /locus_tag="PKM72_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNP60236.1"
                     /translation="MRVHEIAKKIGMESRLLIPELVRLGIQVSSHSNTVDDNMAKWAM
                     NIILGKTPETTPKPGESSVSGHAGVKSSEGGRLLKKDSSTSGRARSEAVAESPKKSEK
                     KHILIKKKKTEEEILEEMRESSPVGEAEAEEPLVIHGESEAPLDTAPVLTQTPGELAV
                     EPPQDAHALLPAESGELPGGVGIPPVPGVESGAIPPSDKTSEKERKSEGKPDKKSAVP
                     SREVPVEEKGKKIKKVARVKDEDVFAARFEDAAVWQDLRPLPTLRREERTRQVQQPSV
                     GEVTKPRKKVMKVTAGISVKDFAEVIGQKPTEIIRKLLDLNIAKTLNQPMDLEAGVLI
                     AEGYGLAVEAVAAKGGEALLEEVIDGGEQGNLEPRAPVVTVMGHVDHGKTSLLDAIRK
                     TQVTDREAGGITQHIGASFVKAGERGVTFLDTPGHEAFTAMRSRGAQVTDIVVLVVAA
                     DDGPMPQTIEAINHAQAANVPIIVAVNKMDKPGANPDRVKQSLAEHGLQPEAWGGQTI
                     FVEVSAKQNQGLDQLLDMLLLQAEIMELKGDSTCSARGVVLEAKLDKGRGPVATVLIQ
                     AGTLRIGESFVVGSVSGRVRGLTSDKGERLKEAGPSIPVEVIGLLGVPEAGDQLVVVK
                     DERAAREIAQARHQKQKDVGLSSTSRRTLEELYAESKDGEIKELPLLIKADVQGSVGA
                     LGDALLKISTDAVKLKLIHSGVGGINETDVLLAAASKAIIIGFHVRPDSKAAAIAERE
                     GVEIRLYTIIYNILDDIRSAAEGLLAPTIRERVMGHAEVRQLFSVPKMGTIAGCYVND
                     GSISRSNTKVRVVRDQVMVYEGKIGSLRRFKDDAREVQQGYECGIGVENFNDLKIGDV
                     LEAYVLEEEATKL"
     gene            complement(13879..15084)
                     /gene="nusA"
                     /locus_tag="PKM72_05315"
     CDS             complement(13879..15084)
                     /gene="nusA"
                     /locus_tag="PKM72_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNP60237.1"
                     /translation="MKSELMLVIDQIGREKGIDKAKVILALESALLTAAKKRFGHGEN
                     IQVDIDTETGEISIVKKKTIAENVLDSKTEISLEDARKIDDEAEMGDEIGSLIELEEF
                     GRIAAQAAKQVICQKVREAEWESIEREYSKKEGELVHGVILGQERRNYLVDIGKTEAL
                     LPIQEQIPREAHRRGDRVRALLLEVRRTPKDVQVILSRAHPQFVVKLFGLEVPEISEK
                     IVEIKGVVREPGDRTKISVASRDKAVDPVGACVGVKGSRVQAIVRELHGEKIDIIPWT
                     NDPRVFIGEALNPASIEKVGIDEQKKSALVVVADSQLSLAIGKNGQNVRLAAKLTGWK
                     IDIISSTEYEKQKLERDLEIKAALAEEASQITEEPAGNAKEPEVASQDENMDNPAVVP
                     TESGQSSGS"
     gene            complement(15157..15636)
                     /gene="rimP"
                     /locus_tag="PKM72_05320"
     CDS             complement(15157..15636)
                     /gene="rimP"
                     /locus_tag="PKM72_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNP60238.1"
                     /translation="MAFTADNLEQVIRQVGEPIARALGVDIFEVQCTGRPANLLVRLT
                     IDKKGGVGIEDCEQFHQSLRRTWEVLHPEQAAYRFEVSSPGLDRPLRDPKDYERVVGE
                     RLRVTLKSSIKKQSVVLGQLITVTDMGIHLMDDQTKNPQEVVVAWDDIAKARLEVSF"
     gene            15906..16835
                     /locus_tag="PKM72_05325"
     CDS             15906..16835
                     /locus_tag="PKM72_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNP60239.1"
                     /translation="MPCIVSVASGKGGVGKSMVVSNLGLLLAKKGLRVTLVDMDIGGA
                     NLHILFGMFHPPSTLSDFLTNTLKNLNETAHPIPGPSSLKLIPGTGETLITANLQHAK
                     KKRLIRHLHKLEADIILVDVGAGTSYHALDFFLLADFFLAVATPDPTSVLDLYRFIKL
                     AAIRKVLTAFSARDPVAESLLDKDFHSIAAVLEAVGKTSESGVATAQEALKRFQPALI
                     LNRMTPKSRINTLHLQHILKQYVGTDLSILGKIPEDIQVQQSILKYLPVVELAPSSPA
                     TNALKQIADNVLEMVEQTPELIERMEEPRKIRA"
     gene            complement(16987..>17204)
                     /locus_tag="PKM72_05330"
     CDS             complement(16987..>17204)
                     /locus_tag="PKM72_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60240.1"
                     /translation="ERLNSQCMEGEEDRIDALRSLQSAQAMIDSLTKELEALKRESEV
                     AQQHCRVLEKKVQQLQESLAENSGTSS"
     assembly_gap    17205..17304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17305..17906)
                     /locus_tag="PKM72_05335"
     CDS             complement(<17305..17906)
                     /locus_tag="PKM72_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNP60241.1"
                     /translation="MRNRISCSAQELSQLDADMLQSVVGGTVFEEGHQYFSAQRVKIL
                     DADQTQITAEVNGVYGVYTQIIKLRAGTLSTRCSCPSNEQPFCRHCVAVLLHQFHNGS
                     SLKPGPKEDPKDQAPPPSDPRIRPAGPYTDESEGAGDLNFWEAILFIDWVQKAAGLLG
                     KEATLPPVPGSLGGVAREWVGMFERLNSQCMEGEEDRIDA"
     gene            18096..19664
                     /locus_tag="PKM72_05340"
     CDS             18096..19664
                     /locus_tag="PKM72_05340"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP60242.1"
                     /translation="MNLATSTGHTTPASFLESLLAEAPETLPILRESCIQVLNLTQDK
                     RSSASDIGEIIMRDQAMMANVIKIANSPVYHTRTPVKTPTYAVALIGFDVIRAMVVSA
                     QLIEQADTFGANTVNLKHLLARALVAGTQAQELGKAINYGEAGSLFTNAMLYSLGDLI
                     LTLCRPDVGEQLEAIRREDPAKVSKAELTLLGRPLHVIAAAMAKHWNLPESLVQLLEK
                     KPVWPKSRPEADQRIMESIVRAANELSYCLLNPLAATQGEVLQSLIEQFLPPFGLSMI
                     QLEHTVSQAFSQASEIASAINIDRQHFLPASDADGTILHNQHLHRLTQTIHQVLHSEG
                     ILSEETIQPAQEASSSLPPSPASDRPLLDFTIQAMKISEPSSLLTFVTRTLYASYGFE
                     RVWLALVVPGKDTLQAKIGYGPHAETIQHTFHCPLSHGTFWDRLLHRFHPIQYSSLVK
                     EGESGGMPTDFLKYWGDPPGFAGTLYAPNRPIGVILVDRGSTGHPLTDTDFATFAMVL
                     SQTNANLARLTQQH"
     gene            complement(19850..20554)
                     /locus_tag="PKM72_05345"
     CDS             complement(19850..20554)
                     /locus_tag="PKM72_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60243.1"
                     /translation="MTKVLYSIGYATPIVTVLGLFICYLMAPEFYLTHILEEVQREQG
                     AVEIVTFWSAMMGGSLLLWSSWKFWKNGNWPAAGVIGAVSAATLLFAGEEIGWGQIYF
                     GWDTPLWWSTRFGGSTDLHSSRFPAHTVAAIFLFVIFILLPLVWKFQLPLRLPANLKP
                     AIPEGPVIFAIVFATLYRELKGFYFWLTPLGYRDRFYEEFLWGLNEHKEMLIAIAMLL
                     YALYRLPHLKQIRSPS"
BASE COUNT         5154 a         5763 c         4404 g         5226 t
ORIGIN      
        1 agtcaaagct tctggaagtg atgacagagc ccatgccgga atcgtcccaa cacctattgg
       61 gaagtgagac ctatccagcc cttctggctt tcgcagctac aaaatcagct ctggctttcg
      121 gacctggtat ggggatttct cctgaaacaa cagaggtctt gcttcaactc cttcctcaac
      181 tgaaggcacc ctgcgttctc gatgccgatg ccctcaatgg attagcgcaa cattgccaaa
      241 tattttcatc catgaaacaa ccgcctatcc tgaccccaca tcccggagag atggccaggc
      301 tacttggagc ctcatcccca aaacaaatta atgaggatcg catcggcgta agtagagagt
      361 ttgccatgca acatcaggta atcttggtac tgaaaggtgc tcgaaccatc atcgctgaac
      421 cccgtggaca ggtggccatt tgtccaactg gcaatcctgg aatggcttcc gcaggaatgg
      481 gcgatgtcct gaccggaatc attaccggtt tgttagctca gggcttgagc ggttgggatg
      541 cggcaagggc tggcgtctac ctgcatggat tggcgggtga cctggctgcc gccaccatcg
      601 gtgaaccagg gctgatcgcc ggggatgtgc tgaccgctat tcctcatgcc ttgactcata
      661 ctctgtctca ttcgtaggag cttccgatcc tgaatccctc tcacgccacg cctcagacct
      721 caagcaacga tgcccttcga ctacggatga catccgccca ggaaaccata aaattcggtg
      781 aacggttagg aaaactgtta accggaggag acgtgctggc ccttaccggt gacctgggag
      841 caggaaaaac cgttctcacg tgtggaattg cgctggggct gggaattcca atggatcaag
      901 tcagcagtcc cacgtttacc cttattcagg aatattcagg cgctattccg cttattcatg
      961 tggatttata tcgtcttgat gggccatccg atatctctac attaggatta gaggaatatt
     1021 ttactccaaa caccattgtc ctcattgaat gggctgaacg gttccctcag atattacccg
     1081 ccgaccacat aaccatttgc ttggaatatg gagaggcgga agacgtccgc ctattcaccg
     1141 tatctggaag tggccccagg agtatccgca taggggccaa ccttcaagaa aatttgccaa
     1201 agccacattg actcaactta tcgataccgc ctacaactga acctccattg tcttgtggga
     1261 atccggtccg aataccataa aaaacaaggg ccggcattga cccataactg aaatggccgg
     1321 cgtactcatt ttcaacgatt caccttacac ccaacaataa gaaagactaa aacggctcat
     1381 cagatgaaag accaagaact ctcccccctc atgcgccaat ttcaggatgt gaaggctcaa
     1441 cattctgatg ccattgtatt tttccgtgtt ggggattttt atgaaatgtt ttttgaggat
     1501 gccgaagaag cctcgaaact ccttggcatc gtgttaaccg cacgaggcaa ggccaaaggg
     1561 gagggcatcc ctctttgcgg agtcccctat cacacttcaa ccggatatat cgcaaaactc
     1621 ttgaaagccg ggaaaatagt ggcgttatgc gaacaagtcg aagaccccaa acttgccaaa
     1681 gggctcgttc gtcgtgaagt cgttcgggtc tatacccctg gaaccttgta cgatcatgaa
     1741 ctccttcctt cgcgagaggc caattttttg tccgccctgt gttacacccc gttatccaac
     1801 cagggaaata ccacgctagg caatcgcttt ggactggcct ccatggacct ttcaaccggg
     1861 gaattttgga tttcagaatc cttgatgaag cattccctgc aagagatcgt cgatgaactc
     1921 gtccgccttg agcccaaaga agtcatcttt cctgccccac ttcaagagga aatgacatcc
     1981 gcattccaat ccctgcctat tgcccgcatc gtgcctcgtg atgccacggg ctttgatctt
     2041 gagtcctgca ggacgatcct caccagaaca tttcaggtca atcaggttga agatctccag
     2101 ctccatggac tccgatccgg ccttcaggcc gccggtggcc ttttacagta cctggctgaa
     2161 acccagccaa cactggcaca tagccatata cgacgccctt tgattcgaat cctcgaacat
     2221 gaaatgcaat tggatcaggc caccctgcgt aatttagaag ttctcaagcc tgtatccgaa
     2281 cagcgccaaa gtcctacctt gctcaccaca ttggacaaga ccaaaacacc aatggggaca
     2341 aggcttttgc ggcagtggat cgtccgtcca ttgacccagg tcccggccat tcacttgcgc
     2401 caggaagcgg taaaggaatt ggtcctgaat ctcggggttc gcatgtcgat tcgtgaccat
     2461 ctgaaaacgg tgcaagatct ggagcgactg aatagccgaa ttgtcctgga agtggccaat
     2521 cctcgggacc ttatcaatct ccatcgctct ctggaggatt tggtggtgct tcagcaactc
     2581 ttaccgtctt tccagtctcc catgctgaag aatgtccatg aggcctggga tcccctcctg
     2641 gatgtctcct cctggattgc cgacaccatc attcctaatc cgcccctttc cgtgaaggag
     2701 ggaggcataa ttcaagcggg ggtgaatggg gaactggatg aattacgagt cctggccaaa
     2761 gaaggaactc gtttactgac cgaaatggaa actcgagaac gcaccagaac cggcatcgac
     2821 tctctcaagg tgaaatacaa tcaagtcttt ggctattaca ttgaagtgac aaaagcgaat
     2881 ttatcgcgag tccctgggga ttaccaccgg aaacaaaccc tggtcaacgc cgaacggttt
     2941 accacgggtg aactgcagga tctcgaaggt cggctttcca gtgcggacca aaaaatgaaa
     3001 agcctggaaa accaattatt tgggcagatc cgtctccgtg tcgcatctgc caccaccaga
     3061 attcagggaa tggcggaaca attggctacc ctggacgttt tgacagggct ggcagaagcg
     3121 gccagcatca accggtatca ccagcctact gtccatgaag gaggaacgat tcagatcacg
     3181 gacggtcgcc atccggtcat tgaacacatc cggcaaagtg aaggatttat tcccaacgat
     3241 acaacgctag acttggacac caaccgactc ttgttaataa ccggtcccaa tatggctgga
     3301 aagagcacat acttgcggca ggttgccctt atcgtactca tggctcagat gggaagcttt
     3361 gtgcctgctg agtctgcacg aataggaatt gttgatcgta tttttaccag agtaggagca
     3421 gccgacgacc tcagtgccgg tcaaagcacc tttatggtgg aaatgagtga aacagccaga
     3481 attttggaca cggcgacttc ccgaagccta ttgttgctgg atgaagtagg aaggggaacc
     3541 agcacctatg atggactgag tatcgcttgg gcactggccg aatatatcct ggaccgtgga
     3601 catctcggag cacggactct ttttgccacc cattaccatg aaatgaccca attagagacc
     3661 atcagagaag ggatcaaaaa ctacacggtg cttgttcagg aaaaagggca agatgtcctg
     3721 tttctcagaa aaatcatcca gggaaaggct gatcgaagct acggaataca agtcgccaaa
     3781 ctggcaggga taccaaaacc tgttcttgac cgggcaagaa atattctagc ccaacttgaa
     3841 caggattcct catctaacaa cgtgtccctc tccgatgagt cagtggtgga tacccctccc
     3901 ctcgaacagg tctcaccaaa accccatgtt atcttagatg aagtcaaaca gatggatctc
     3961 ttttccatga ctccgcttga agccctcaat cgactagctg attttaaggc tcgattgaat
     4021 gagcacaatc cctaataatt tttaagaacc ccacaccaca aaaaaaaagg gcctgcgtta
     4081 tacgcaggcc cctttacaaa acctttaatc taaaattgtc taaaggttat cgtctttttt
     4141 aatgcttccc acccatttcc ttgtgcactt ctaccttctc aaacgaagct acggctggat
     4201 cgtaaatctg gccggtccct tgatcggagt aaggaatgag tccaacaacg gctttaccat
     4261 ctggaatcaa cacatcatag agcattttgc gttgatctgg ccaagaatca gcccgtatgg
     4321 catccaaaac cggcgtggta cgaagagtag aaatatttcg tggcttatca ggagtcccag
     4381 ctacttcctg aagggacttg gccgaagaca caacccggtg gttttgctcg gtttcttgaa
     4441 ccacaatttc caccaaacca taatttgatg gcttcgtgtc tgtcatcaca gcctgacttg
     4501 aagaatcttt cccggccgct acaagtgttt tccaaatttc gtcgctgcct gggtacactt
     4561 tgagattttt gccaggataa tacttttgcc agtaataacc gctttccgca ttcccaccaa
     4621 acacggccac attgatccac accgctttat cgtatggatc gaccacagcg acccgcccct
     4681 ggagggttgt gggcttactt aaatcacccc actcaaatcg ctcttgaaaa gcttcgatgg
     4741 ccattgcgct tgaggccatt gccccaacta gcgccacggc tccaacaagg gccaaccctt
     4801 tctttttagc acctttcata atggcttccc tcctcgagta aagttttaac tctcaatcat
     4861 gatttatctg acaaaagttt attctgcctc taacaattta aatcccgtaa tcgtacgacc
     4921 gcttaattgc acttccatgg ccacaagctc ttgagctgcc ttggtgagtt ggtccattaa
     4981 agctttatcc ttcacagcca accgaacggt agtttgggga ttgtaccaac cgccataagg
     5041 attgtttttt tcattgcctc ctgtataacc ccatgcgcag cagatataca atggatcagg
     5101 aagagcaacg ttgcgatcat cagtagcccg gcctggagga tcggctgttt cttgtggtcc
     5161 agatgcgttc caatactgta tcccgaataa aatttctccc tcagggttgt ctgcccattg
     5221 tgcatacatc cggcccttca aactggtgac tgcctcccct ttaaaagaaa gatcaccctg
     5281 gctggtgaca tcatttgggt cgcccttcga cttatcagga cctggtccag cagcataccc
     5341 aatgcttgct aatccaagac ccaacaggcc catggcacag agagctacca attcatgacg
     5401 tagagatgac ttcttcatct gctccctcct tacagtgtta gaatgacaaa aattaaaaaa
     5461 caacttgacc tttgtttatc tgctaatcga ctttcccagc ttccataccc ctggttctta
     5521 aagggtaaat cagagtttta aaagggtccc atgctaccca tccagggcat ggttgtcaag
     5581 cggaaaaaga tgcgacttat gactcatccc cacctcggaa ttcccctttt cgggataggt
     5641 cccaatgccg taaccacaaa ttgttgtaga tcaaataatt tctggcttaa atggcgaatt
     5701 tgatcagggg ttatccgatc aatggccttg accatttcct gaagggtgac atgacgccct
     5761 tgatacattt cgtcttttgc caatttattc atccgcccgt aggttccttc caatcccaac
     5821 atcaaccccc cctttaattg ggttttggtt cgctccaatt ccttactgac cacgtcccga
     5881 cggcataact tcttggtttc ctgacaaatt cggtcaatga cggctgtaac ttcattcgga
     5941 cgagtggcgg cataaacggt aagcgtcccg ccatctaaaa agcctgaaag atgagaatat
     6001 atggtatagg ccaacccacg tttttcacgg atttcttgaa ataatcgcga actgactcct
     6061 ccgccaagaa ttgtgcttag gacatgaccg gcatatcgat ccggatgtcc tatcggcaac
     6121 cccttaaatc cgacacagac atgaacctgc tccagtggtt tcacgtgaag actttgccgt
     6181 tcctgcggct gatcaggcca tggcgtttcc tggatggttg agggatgctt gtcgaacttg
     6241 ctcctcttcc attggccaaa atatttatta acggtatcaa tgatttctgg aaaggaaaag
     6301 ttaccggcca cggcaacgat cgtgtgttcc gggcggtaat gctgttgtcg atacttcatg
     6361 agcaattggc gatcgagtcg cttcatcacc gcaggctctc ccaagatcgg acgccccaac
     6421 ggatgggagc caaagatatc cttggcatgc aattcatgaa tatagtcctc gggatcatcc
     6481 tgaacggtac ggatctcctc aaggacgatt tgtttttcct ttgcaatgtc tttggcagaa
     6541 aaccttgaat ggtgaaacaa atccgcgatg agatcaaatg ccagccccat ttgctgatcc
     6601 aacaccttca cgtaaaaagc cgtggcctca tgggtggtaa aggcattcat ttcaccaccc
     6661 aaggcatcaa tctcattgga aatctgagag gcagtccgtt tcggcgtgcc cttgaacatc
     6721 atatgttcaa tgaaatgaga gaggccacct tcccctttcc cttcgtcacg acttccaacc
     6781 gtggcccaca ctcctagggc aacggatttc aatgacgaca tccgctccag gacaacacgg
     6841 actccgttat ccaatatgac ttttttatac acgcttggtt actcggaccg gcttatgagg
     6901 tgagctgatc cttgtccgtc tgttcggcca tggcatcttt tcgactaaga cggatcttcc
     6961 cttgtcgatc gatctccagg actttcacaa gaatctgttc gccttcagaa atttcgtccg
     7021 tgaccgatgc cacacggtga ggagccaatt gcgaaatgtg cacaagccca tccataccag
     7081 gaagaatttc cacaaaggcg ccaaaatcta caatttttcg caccgtcccg aggtaaattt
     7141 tcccaatctc cgcttcttca acaatccgct gcaccatatc catcgccttt tgcgccgact
     7201 ccccgtcaac cgcagcaatc gtgaccactc cagagtcatc cacattcact ttcactccac
     7261 aatctgcaat gatgctacga atcattttgc ccccaggccc aataacatcc ctgattttat
     7321 cttgcttgat ctggatcgtg gtgatacggg gtgcataggc cgccagctcg gtacgaggta
     7381 ccgtcaggca ttcatccatt ttcccaagga tgtgcaatcg cccttcacga gcctgcgcca
     7441 acgcctgccg catcaggtct ggggtaattc cggcaatctt aatatccatc tgcagggctg
     7501 tgactccatc tcgcgttcct gccactttga aatccatatc gcccagatga tcttccatac
     7561 ccaaaatatc tgaaagaatc gcgacgccac cctgttccaa aattaacccc atcgcaattc
     7621 ctgccaccgg cgtcttaatg ggtactcccg catccatcat ggctaatgcc ccaccacaaa
     7681 tcgtggccat ggaagaggac ccgtttgatt ctagaatatc cgagacaagc cgaatggtat
     7741 aggggaattc ttctttggaa ggaaggacgg cctctaaagc cctttgggcc agcttgccat
     7801 gcccgacttc ccgccgtccc ggccctcgga gcatttttgc ctcacctacg ctgaaaggcg
     7861 ggaaattata atgcaacata aatgagcgga aatactcgcc ttccaaggca tcaatccgtt
     7921 gctcatcatc tgaagtgccc aaggtcacca cagccaaagc ctgtgtttcc cccctggtaa
     7981 ataacgccga accgtgcgtg cgaggaagaa tccctgcctc agaggatatc ggacgaatgt
     8041 cggatagacc ccggccatca gctcttttct ttgtttccag gataattctc cggacttcgg
     8101 aatattccaa gttagcaaag acttttttca ccttggcttt tcgatcggca tcgtctccgg
     8161 caactgcctc aatggcttct tggagaattt gagcgaatcg atcttgtcga tccgctttgg
     8221 ccgcgatata catcgcttca ccaataggag cggctgcgat ttttcgcacg gcttgttcaa
     8281 tttcagggtc aatggcttcg acctgcaaca cccgtttgac tttccccgcc tttgcccgta
     8341 attcttcaat tttggcgacg atattcttga tttcacgatg agccagttcg atcgcgtcaa
     8401 tcatcgtgtc ttccgaaagg ccatccgcac cagcctcgac catcatcacg gcgtccttgg
     8461 ttccagccac gacgaggttt aactcacttt cctcctgtgc cttcttatcg ggattcacca
     8521 cgaattggcc cttcactcgt ccaattctca ctcctgccag aggctcggag gctggaatgt
     8581 ctgaaatggc cattgctgcc gacgcggcaa caataccaat gacgtccgaa gacatggtct
     8641 gatcaataga gagcacggac acaatgatct gggtttcaaa aaagtaacct tccggcatca
     8701 atggccgaat cggccggtct atgagtcggc tggttaacac ctctttttca ctgggtgccc
     8761 cttcccgttt gaaaaatcct cctggtattt tcccggctga gtaggctttt tcttgatagt
     8821 ttaccgtcag aggcaagaaa tcggcgtccg gcttcgaggt ctttgaggct accgcagtag
     8881 ctaacaccac cgtatcggca taggtcgcca atacggcacc atccgcctgc ttggccattc
     8941 gaccggtttc caaacgaagc gggcgaccac caacttccat ttcaacgaca tgaatcatgt
     9001 ttcaaccttt ctgatccttg agtggatata ttgatatgga gtgtgaggct tcgcttcgca
     9061 ttgagaaccg ggcatctatc acccatctca ttgattctgc tcaccctaaa acctgcattc
     9121 tgtcttattt ccgtatacca agtgcagcga tcacggcttg gtatttgccc tcgtctcgac
     9181 gccgcaaata atccaataac tttcttcgct tgcttaccat tcgcaaaagg ccccttcggg
     9241 aatgatggtc ctttttgtgt gtcttgaaat gttcagtcag actcgtaatt ctattggtta
     9301 acacggaaat ctgcacttca gacgaacccg tgtccttgtc gtgaatccga tgcgattcta
     9361 tggccgccgt ttttgcttct ttcgttaagc ccataactcc tcctccagtt cctcagggtt
     9421 acatgagttc agtaagaaaa tctttctacg gatgtcgtct tatccccact taatcgaacc
     9481 taaattttca taaactagga aaacaccgtt tcaatcgcta atacgggacc tcccttgcct
     9541 gtctgaaaaa ccgggcctct ccctaacgcc agcaattgcc catctttctg tttcaccctc
     9601 accatatttc cctccttggg tgagactcct gattccacaa acgtgtgaac cacacgatcc
     9661 caggaaatcg cattcccatg gagcaccttt cgagcatcat cgctatcgac ctcaacagcg
     9721 tgaaacccct ctaaggcctg atctaaactc aaaaatgccc catccagatg agaaaattca
     9781 agtcccccaa gcaaggattc tacctcaagg gcctgatcaa catgaatagg tccgacccgt
     9841 cgacgctgaa gccatttcaa atgtccaccc acctgcaata cgtttccaat atcatcgcag
     9901 agagttcgaa cataggttcc cttagaacag accactcgaa acgcgacctc agggatattc
     9961 agtctttgaa tttctaaatc atgaatcacc acggttttag cgggtcggtc aacggtctca
    10021 cctttcctgg cttttttata caagggttgc cccccgattt tcacagccga gtacattggt
    10081 ggcacttgct ggatagaccc ttgaaacgtc gcaaaggctg accggatacg atcctctgtc
    10141 aaagattcta tggggaattc ctctaagacc gtaccccaag catcttgtgt gtctgtccgt
    10201 tggcccaatt gaagcacggc gccatactct ttttcccatc ccaacagata ctgggcaatt
    10261 ttggttccct ttccaattaa aaccggcagg acgcccgtcg catggggatc caaggtgccc
    10321 gcatgcccga cttttggaat acccaaaccc gacctgaggc gttgcaccac atcatgcgat
    10381 gtccatccat caggcttatt cacattcaga acgccatgta aggccattcc ctgcggtgcc
    10441 ttcaagcctt tagactggtc gatgcacgaa gccattggtg ccattacccg tctatacttt
    10501 cgctcttgtc atggagaaag gaggaagcct cagctttacc ggcttcatca gattcctcat
    10561 gaagagaatc cagcaacttt tcaattctgt tcccatactc agcactcgta tcacgataaa
    10621 aaaccaattc cggtgtataa cgcagattta agcgcctgcc caattctgtg cgaacaaacc
    10681 ccacagcgct tttaagaccc ggccctgtaa cctggtcaaa atgggcttga tccaaagcgg
    10741 tcacatatat ccgtgcgatt ttgagatccg ccgtcatctt cacacctgtc acggtcacaa
    10801 attggatacg tggatcttta gtttttctgg aaagaatctc tgccacttcc atccggatct
    10861 gatcggcaac tctcgtcgcc cgactgctat ttgtttttgc catgacgtga aaaatttata
    10921 tgagagctta caagacttca atttgagatc gtaaaagctc gatggaggga ttagatcgta
    10981 taaagttgag gacctggtct aagacttgat tgacccttcc cgtgtcattc gctacacacg
    11041 ccactcccaa aatggctttt tgccaaatgt cttgttcctc aatttcagca acggaaatat
    11101 taaaccgatt tcgcaatcgg gtaatcaagc ttgagagaac ctgacgctta cctttcaggg
    11161 attggacatc agggatatgg agttcgagcg tgcacaaccc cacgatcatc cccattcagg
    11221 atccccttat ccattctgga ttataatttt gtggcctcct cttccaagac gtaggcctct
    11281 aacacatcgc cgatttttaa atcattgaaa ttctcaactc caattccgca ttcatagcct
    11341 tgctgaactt cccgtgcatc atccttgaac cgccgtaacg atccaatttt tccttcatag
    11401 accatcacct ggtcacgaac aactcttacc ttggtgtttg aacgggatat cgacccatca
    11461 ttcacgtaac aaccggcaat ggtacccatt ttcggaacag agaatagctg ccgaacctcc
    11521 gcatgcccca tcacccgttc cctgatcgtt ggagccagaa gaccttctgc agccgaacgg
    11581 atatcatcaa gaatattata gatgatggtg tacaaccgaa tttccacacc ctcacgttca
    11641 gcaatagcag ccgcctttga atccggacga acatgaaatc caatgataat cgcttttgaa
    11701 gcagccgcta acaacacatc ggtttcgttg atgcctccca ctccactatg aatgagctta
    11761 agctttaccg cgtcggtaga aatcttcaga agtgcatcgc ccaatgcccc cacggatcct
    11821 tgcacatccg ccttgatcag tagcggcaac tccttgatct ccccatcttt tgattctgca
    11881 taaagctctt cgagagttct tctggacgtc gatgataggc cgacatcttt ttgcttttgg
    11941 tgccgggcct gggcaatttc cctcgcagcc cgttcatcct tcaccacgac cagttgatca
    12001 ccggcctcag gaacccccaa taagccaatg acttctaccg gtatcgatgg cccggcttcc
    12061 ttcagacgct cacctttatc cgatgttaac cctctcaccc ggccgctcac tgacccgaca
    12121 acaaacgatt ctccgattcg taaggttccg gcctgaatca acaccgtggc aaccggtcca
    12181 cgccccttat ccaacttcgc ttctaatacg acccctcgag cggaacaggt ggaatcgcct
    12241 ttcaattcca taatttctgc ctgaagtaac aacatgtcaa gcaattggtc taacccctgg
    12301 ttttgctttg ctgatacttc aacaaaaatt gtttgacctc cccatgcttc aggttgcagg
    12361 ccgtgttcgg ctaaactttg cttgacccga tccggattag cccccggctt atccatttta
    12421 ttcacggcca cgataatcgg cacattggcc gcttgcgcat gattaatggc ttcaattgtc
    12481 tgaggcatcg gcccatcgtc tgcggccacg accaagacca caatatcagt gacctgagcc
    12541 ccacgggacc gcatcgccgt aaaggcctca tggcccgggg tatctaagaa cgtcacgcct
    12601 cgttcaccgg ctttcacgaa ggacgcacca atgtgctgag taatccctcc cgcttcccgg
    12661 tcggttacct gggtcttccg aatcgcatcc aacaaggagg ttttcccatg gtcgacatgt
    12721 cccataaccg tcaccacagg cgctcgaggt tctagatttc cttgttctcc accatcaatc
    12781 acctcttcca ggagggcttc ccctcccttc gcagccacgg cttccactgc gaggccataa
    12841 ccttccgcaa tgagcactcc agcttctaaa tccatgggtt gattaagggt ttttgcaata
    12901 tttaaatcca acaatttccg aattatttcc gtgggttttt gaccgatcac ttcggcaaaa
    12961 tctttcacgg atatcccagc agtgaccttc ataacttttt tcctgggttt tgtaacttcg
    13021 cccaccgaag gttgttgaac ctgacgggta cgttcctcac gtcgcaaagt tggcaacggt
    13081 cgaagatctt gccagaccgc ggcatcttca aagcgtgcgg caaagacatc ttcatctttt
    13141 acacgcgcaa cttttttaat ttttttgcct ttttcttcta ccggaacctc tcgagatgga
    13201 acggcactct ttttatcggg tttcccttcg ctttttctct ctttttcact ggttttatca
    13261 gagggaggaa ttgcgccgct ttcaacccca ggaacaggtg gaatccctac accaccagga
    13321 agctctcccg actcagcggg caataaggcg tgggcatctt gaggaggctc cacagccaat
    13381 tcgcctggag tctgagttaa aacgggagcc gtatccaagg gagcctctga ctcaccatgg
    13441 atgaccagtg gctcttctgc ctcagcttcc ccgaccggag acgattccct catctcttcc
    13501 aggatttctt cttctgtttt tttcttttta atcaggatgt gttttttttc ggattttttg
    13561 ggactttcgg ccactgcttc ggaccgagcc ctcccggaag tcgacgaatc ttttttcagt
    13621 aggcgccccc cctcagagct tttcacaccg gcatgccctg aaacagacga ttcccctggc
    13681 ttcggggttg tttcaggagt tttaccaagg ataatattca tcgcccattt ggccatattg
    13741 tcatccactg tattgctatg ggaactaacc tgaatgccaa gtcgcactaa ttcaggaata
    13801 agcaaacggc tttccatccc aatttttttg gctatctcat gaactctcat gcacgtcctt
    13861 tactgtgaag ccgtatcctt acgatcccga actctgccca gactctgttg gtaccaccgc
    13921 gggattatcc atgttctcat cttgtgaggc tacttcaggc tccttggcat ttcccgccgg
    13981 ctcttctgta atctgtgaag cctcttcagc caaggcagcc ttaatttcca agtcgcgttc
    14041 aagcttttgt ttttcgtatt cggtagagct aatgatatca attttccaac ctgtcaattt
    14101 tgctgctagg cgcacgtttt gcccgttctt cccaattgcc aaagagagct gagaatcagc
    14161 caccaccacc aacgccgatt tcttttgttc gtcaatcccg actttttcaa tggaagcagg
    14221 gttcaaggct tccccgataa acacgcgtgg atcatttgtc caggggataa tatctatttt
    14281 ctcaccatgc agctcccgaa caatggcctg gacacgtgaa cctttgacgc ccacacacgc
    14341 gccaaccgga tcaaccgcct tatcgcgtga cgccactgaa attttcgtcc ggtcgcccgg
    14401 ctcacgcacc acgcctttaa tttcaacaat tttctcagat atctcaggca cctccaaacc
    14461 aaacaatttt acaacgaatt gaggatgcgc ccgggagagg atcacttgca catccttggg
    14521 ggttcgtcga acttctagca acaacgcccg cactcgatca ccccggcgat gagcttcacg
    14581 cgggatttgc tcctgaatcg ggagaagggc ctctgttttt ccaatgtcca ccagataatt
    14641 tcgccgttcc tggcccaaaa tgaccccatg aactaattcc ccttcttttt tggaatattc
    14701 ccgttcaatc gactcccatt cagcctctcg aactttttga caaatgacct gtttcgcagc
    14761 ctgtgcggct attcgcccaa attcttccaa ctcaatgagt gagccgattt cgtctcccat
    14821 ttcagcttca tcatcaattt tcctggcatc ttccaatgaa atctctgttt tggaatccag
    14881 cacgttctcc gcaatcgtct ttttctttac aatagaaatt tcgccggttt ccgtatcaat
    14941 atcgacttga atattttcac catgcccaaa tcgttttttt gcggccgtga gaagcgcgga
    15001 ttctaaggcc aaaatgactt ttgccttatc aatccctttc tcccgaccga tttgatcaat
    15061 gaccaacatg agttcacttt tcatttgtat tcctcgcaag gcacgatgag cggttcaaac
    15121 ggacttgggc gattggtgcg cgcttccaat aaaacgttaa aacgaaacct ctaatctcgc
    15181 tttggcaata tcatcccacg ccacgactac ttcttgggga ttcttggtct ggtcatccat
    15241 taaatgaatc cccatatcgg tgacggttat caattgcccc aaaacaactg attgtttttt
    15301 gatcgagctt ttcagagtga ctcgaagtcg ttcgcccact actcgttcat aatcttttgg
    15361 atctcggagc ggtcgatcta gcccaggcga agaaacttca aaccgatacg cggcctgctc
    15421 tgggtgaaga acctcccagg ttcgacgcag ggactgatgg aattgttcac aatcttcaat
    15481 tccaactccc ccctttttat caattgtcag tcgaaccaaa aggttggcgg gtctcccggt
    15541 gcactgaacc tcaaagatat ccactcctag agctcgagcg atcggttcgc ccacttgacg
    15601 aataacttgc tcaagattgt cagccgtgaa ggccaccaca aatcctcgct acagcggcca
    15661 caagccaaga ccaaaaaaaa gtgggctcac gcccactcta ctcgcaatca acctagcatt
    15721 ttccattttt aaaggcaaga gctcaccaga ttcatcgcga cgtcccggta tgtcaaaatc
    15781 tctcaaaagg ccatccatac gagcattcct tcaccatggg cataagaagg accctgtgat
    15841 aggctatttt ctgagtaagg tgatacttac ttcctggtga tatcaacgag agaggagttc
    15901 aaccgatgcc ttgcatagtt tctgtggctt ccggtaaggg cggagttggg aaaagcatgg
    15961 tcgtcagcaa tcttggactc ctccttgcca aaaaaggact tcgcgtcacg ctggtcgaca
    16021 tggacattgg tggggctaac ctccacatct tgtttggaat gttccatcct ccatccaccc
    16081 tctcagattt tttgacaaac acgctcaaaa acttaaatga gaccgcccac cctattcccg
    16141 gcccctcctc cctgaagctc attcctggaa cgggggaaac cctcattacc gcgaatttgc
    16201 aacatgccaa aaaaaaacgc ctcatccggc acttacacaa acttgaggca gacatcattc
    16261 tcgttgacgt cggcgcgggg accagttacc acgcactgga tttttttctc ctcgcagact
    16321 ttttcctggc cgtagcaacc cctgacccaa cctcggtttt agatctgtat cgattcatta
    16381 aacttgctgc cattcgaaag gttcttacgg ccttttcagc ccgcgaccca gtggcagaaa
    16441 gccttttgga caaagacttt catagcatcg cagcagtact ggaagccgtt ggaaaaacga
    16501 gcgaatcagg tgtggcgact gctcaagagg ctctaaaacg gtttcaaccg gccctcatct
    16561 taaaccgaat gacacccaaa tccaggatca ataccctgca tttacaacac atactaaagc
    16621 aatacgtcgg aaccgatctg tcaatcttag gcaagattcc agaggatatt caggttcagc
    16681 aatccatcct gaaatatctt cctgttgtag aactggctcc ctcttctcca gcaaccaacg
    16741 cgctgaaaca aattgccgac aatgtacttg aaatggtgga acaaacacct gaattaattg
    16801 aacggatgga agagcctaga aagattcgag catgaatgat taacggacga cattacctta
    16861 gcttttcgta aaaaatccga cggctcgcct ctcatataaa aagattcaaa aaagggggtt
    16921 ccatttcact gagccatgac ccgccctgaa aggcaaatta cccgccaagc ctgctgcacg
    16981 ccctgcctaa ctggatgtcc cagagttctc cgccaatgat tcttgcaatt gttggacttt
    17041 tttctctaac actctgcaat gctgttgagc cacctcggat tcccttttta acgcttccag
    17101 ctccttcgtg aggctatcaa tcatggcttg ggctgattgc aggctcctca aggcatcaat
    17161 ccgatcttcc tcgccttcca tgcattgtga attgaggcgt tcaannnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnaaggca tcaatccgat cttcctcgcc ttccatgcat
    17341 tgtgaattga ggcgttcaaa catacccacc cattctcgag caacacctcc caaggaacca
    17401 ggcactgggg gcaaggtggc ctccttaccc agaagccctg cggctttctg aacccaatca
    17461 ataaacaaaa tcgcttccca aaaattcaaa tcacccgcac cctcggattc atcagtatag
    17521 ggtcctgctg gccgtatccg cggatcggaa ggaggaggag cctggtcctt gggatcttcc
    17581 ttcggtcccg gcttaagaga tgatccgtta tggaattgat gcagtaatac cgcgacacaa
    17641 tgacgacaaa aaggttgctc gttcgatgga caggaacacc tggtcgacaa agtccccgca
    17701 cgtaatttta tgatttgcgt atacacgcca tagacgccat taacctcagc cgtgatttgg
    17761 gtctggtccg catcaaggat cttgactcgt tgggcagaaa aatattgatg gccttcttcg
    17821 aagaccgttc ccccaaccac cgattgaagc atgtccgcat ctaattggct cagctcttga
    17881 gcggagcagg aaattctatt tctcatgcag ataccatcca attcaaaaga aaggagaccg
    17941 acctcacgcc tccgagaaag aagagggttt aatatttcgg aaaatataac cgagaatcaa
    18001 cctttagagc caactaccca gcttaatttc cttgatctaa aaccacctgg cccaggaatt
    18061 accgcctggc actcaaccct ggcggagatg ctatcatgaa tctcgctacc tctaccgggc
    18121 acacaacacc ggcttccttt ctggagagtc tgctcgccga agccccggaa accctgccta
    18181 tcctccggga aagttgcatc caagttctca atcttacgca ggataaacgc tcgagtgcca
    18241 gcgacattgg agaaattatc atgcgcgatc aagccatgat ggccaatgtc atcaaaatcg
    18301 caaatagccc tgtctatcat acccgcacac cagtcaaaac cccaacctat gcggtggccc
    18361 ttattggatt tgacgtcatt cgcgccatgg tggtctctgc ccaattaatt gaacaggccg
    18421 acaccttcgg agccaacacc gtcaacctga aacacctcct ggcgcgggct ctcgtcgccg
    18481 gaactcaggc ccaggaactg ggaaaagcca tcaattacgg ggaggcgggc tcgttattta
    18541 ccaatgccat gctctactca ctgggagacc tcatcctcac cctctgtcga ccggacgtag
    18601 gcgaacaact agaagccatt cgccgggaag atccagccaa ggtctccaaa gccgaactga
    18661 ctcttttggg acgaccgctg catgtcatcg ctgccgctat ggccaagcat tggaatctgc
    18721 cggagagttt ggttcaacta ttggagaaaa agcctgtctg gcctaaatcc cgtccagaag
    18781 cagatcaacg gatcatggaa agtatcgtcc gagccgcgaa tgaactgagt tattgcctcc
    18841 tgaacccatt ggctgccacg cagggagagg tactccaaag cctgattgaa cagtttttgc
    18901 ccccattcgg cctctccatg atccaattgg aacacaccgt gtcccaagct tttagtcaag
    18961 catcagaaat tgcttcggcc atcaacatcg atcgacagca ttttcttccc gcatctgatg
    19021 ccgatggcac aattcttcac aaccaacacc tacatcggct cacccaaaca attcatcagg
    19081 ttctacactc agaagggatt ctttccgaag aaaccataca accagctcag gaagcctcat
    19141 cttccctccc tccatctccg gcatccgata ggccattgct cgatttcacc attcaggcca
    19201 tgaaaatatc ggagccctcc tcgctgctta cctttgtcac cagaacgctc tatgcatcat
    19261 atggatttga acgggtctgg ctcgcattgg tagtaccagg aaaagatacg ctccaggcca
    19321 aaatcggcta tggtccacat gcagaaacca tccagcacac gtttcattgc cctctttccc
    19381 atggaacctt ctgggaccga ttgctccata ggtttcaccc tattcaatat tcttcattgg
    19441 tcaaagaagg ggaatccgga ggaatgccga ctgacttttt gaaatactgg ggggacccac
    19501 ccggattcgc cggaaccttg tatgcgccaa acaggcctat cggggtcatc ctggtggatc
    19561 gaggttctac ggggcatcca ttgaccgaca cggatttcgc aaccttcgcc atggtgttgt
    19621 cccaaaccaa cgccaacctg gctcgattaa cgcaacagca ctaagtacga gacatctcac
    19681 cctgcagttc tgctgttcat agctcctcac cgtcagtatt acgtgacgcg caatggaaaa
    19741 tcatagaatt atatctacac cattctcccc tgaactgatg ggagccagaa aagctgttca
    19801 tgtgcggaga aagtgagaag atcagaacca aagatcgagg tggaatggct cacgacggag
    19861 aacgaatttg ttttagatga ggcagacggt agagggcata cagaagcatg gcgatggcga
    19921 tcaacatttc tttgtgttca ttcagacccc acaaaaattc ttcataaaaa cgatctcggt
    19981 agcccagcgg cgttagccag aagtagaaac ccttgagttc ccgatagagg gtggcaaaca
    20041 caatggcgaa aatcaccggc ccttccggga tggccggttt taaatttgcc ggcaaccgaa
    20101 gaggcagttg aaatttccag acaagcggta aaagaataaa tatcacgaac agaaatatgg
    20161 ctgccactgt gtgagcagga aaccggctgc tatggaggtc tgttgatcca ccaaaacgtg
    20221 tactccacca gaggggggtg tcccacccaa aatagatctg tccccaacca atttcttctc
    20281 cggcaaaaag aagcgtggcg gcggaaactg ctccaatcac accggccgcc ggccagtttc
    20341 cgtttttcca gaatttccag ctagaccaca gcaacaagct cccgcccatc attgcggacc
    20401 aaaatgtcac aatctccact gcgccttgtt cacgttgaac ttcttccagg atgtgggtca
    20461 aataaaattc tggggccatt aggtagcata taaaaagccc aagaacagtg acaatgggcg
    20521 tggcataccc aatactatat aagacttttg tcatggtatt tcatctcatg gagcctggca
    20581 tacaacaaaa taaacatgtg aagggagcga tcccccaaga ataggtaacc ggcaggaaga
    20641 cttgaaa
//
LOCUS       DAORAQ010000036        20609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_470_length_20609_cov_68.266080, whole genome shotgun sequence.
ACCESSION   DAORAQ010000036 DAORAQ010000000
VERSION     DAORAQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 20609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20609
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_470_length_20609_cov_68.266080"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKM72_05350"
     CDS             complement(<1..458)
                     /locus_tag="PKM72_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60244.1"
                     /translation="MVKVLGLVSGLVLGIQTTVCAGVVFHVETTFPNRAEMPSRNDAI
                     TVDGKKLKMTINDAGNTQSTVIYRGDQEEILLINHGDHTWRAMDKATMVNLGQQINPA
                     MEKMQEMLKNMPPERRAMMEKMFAGQGINPAMMGGKVEMQVKKTGESQTV"
     gene            1006..2766
                     /locus_tag="PKM72_05355"
     CDS             1006..2766
                     /locus_tag="PKM72_05355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNP60245.1"
                     /translation="MKHDTSNPPTAPTMKGFSPGFAALGLEAALLTTLEALGYEEPTP
                     IQREAIPPLLAGKDLLGRAATGTGKTAAFTLPLLQRLAHSPKQPNPSALILVPTRELA
                     IQVCEAVQRYGKQQHIAILAVYGGQAIRPQLIALKRGVDIVVATPGRALDHIRRKTLQ
                     LKDLQIVVLDEADEMLNMGFAEDLDAILKQTPATRQTALFSATMPPRIASIARQHLRN
                     PIEIQIIKEPVKAGVAPRVHQTAYLVARPHKVAALARILDMSGPKSALVFCRTRLEVD
                     ELTSMLNSRGYRGEGLHGGMNQAQRDRVMQSFRAGKTELLVATDVAARGLDIPHVSHV
                     VNYDVPSSPEAYVHRIGRTGRAGRAGEAITLVESRERWLLQNIERLTKSKVDMGTLPT
                     VADLQTKRLEQIGVSIQDILKANDFEQFRVVVDTLTEKFDQADVAAAAIQLAYGSKTG
                     DRVEEDIPAIRDRASDTTQTSRRPRRGPSDYPDAPRVLRGGAGGGRPVGRRDRHSQST
                     SMATLHVNAGRMAGVRTGDLVGAIANEAGISSNVIGPIKVSDQFSLVKVPEEHVHGII
                     KALGRTRIKGQKVTIRLYKE"
     gene            2901..3635
                     /locus_tag="PKM72_05360"
     CDS             2901..3635
                     /locus_tag="PKM72_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNP60246.1"
                     /translation="MSNGMTILDVRDRIAMARSVTVLTGAGISADSGVPTFRGPEGLW
                     KNFRPEELASPEAFARDPKLVWEWYNWRRELVATKHPNAAHETLVELERRIEHFWLIT
                     QNVDGLHALAGSQRLSEIHGNIWKVRCTQCNRISSNRDLPLVYPPTCTDCSGLLRPHI
                     VWFGESLDQEDLNRSYQALRTCDILLIIGTSGVVYPAASFAPVAKDGGALVVELNLDP
                     TPNSSVVDVSFQGRAKDLVPLLRQVT"
     gene            3719..3919
                     /gene="thiS"
                     /locus_tag="PKM72_05365"
     CDS             3719..3919
                     /gene="thiS"
                     /locus_tag="PKM72_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNP60247.1"
                     /translation="MQLTINGKPETLEVSTVLDVLKTKDIDPQLVAVELNTQMVDQEQ
                     LGTTSLKDGDKLEFLFFMGGGA"
     gene            3916..4845
                     /gene="cysK"
                     /locus_tag="PKM72_05370"
     CDS             3916..4845
                     /gene="cysK"
                     /locus_tag="PKM72_05370"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNP60248.1"
                     /translation="MSAAYLRNITDGIGHTPLVRLNRLSPAGGATIYGKAEFYNPGGS
                     VKDRICLNMIDEAENKGLLKPGGTIVEPTSGNTGIGLALVSAVRGYKLILVMPEGMSL
                     ERASLLSSYGAQLVLTPAREGMRGSIKEAESILEQNPEYFMPNQFSNPANPAIHRKTT
                     ALEIWEALEGKVDAFVAAVGTGGTITGCGELFKENNPSVKVIAVEPTGSPVLSGGEPG
                     PHRIQGIGAGFVPKVLNRSLIDQIMTVTDEEAYQTTKQLARKEGLLVGISAGANIFAA
                     QKVAEALGPNKNVVTILCDTGERYLSIEKYFNI"
     gene            5056..5886
                     /gene="moeB"
                     /locus_tag="PKM72_05375"
     CDS             5056..5886
                     /gene="moeB"
                     /locus_tag="PKM72_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNP60249.1"
                     /translation="MSFTEDQITRYSRHILLPEIGGKGQKKLSQAKVFIVGAGGLGSP
                     VAYYLAAAGIGTIGLIDSDVVDLSNLQRQILHHTPDVGRSKVVSAKEKIQAVNPDVQV
                     NIHEERMTSRNAQELINQYDVVIDGVDNFPAKFLINDACYLENKPLVHGGILRFEGRV
                     FTIIPNQSACYRCVFKNPPPAGVVPTCQEAGIIGVVAGLIGTIQATEAIKLILNIGTP
                     LTNRILDFDARTTAFREIRVKRNPSCALCGPNPSLTELIDYEQEACQLQPSATTLTNG
                     "
     gene            5918..7156
                     /gene="thrC"
                     /locus_tag="PKM72_05380"
     CDS             5918..7156
                     /gene="thrC"
                     /locus_tag="PKM72_05380"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNP60250.1"
                     /translation="MKKMLALVCRECGKEYPTQDIHVCELCFGPLEVKYDYAVIKQVM
                     TREKITSGPRSLWRYVDLLPVEGTNFIGLHAGFTPLVHAKNLGAFLGLDNLYIKNDCV
                     NHPTLSFKDRVVAIALTRARELGFETVACASTGNLANSVSAHAASAGMKCYVFIPGDL
                     EAAKVLGNLIYRPNVVEIEGNYDDVNRLCSEIAGERHWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWRAPDQVVVPMASGSLLTKIWKGLKEFEKLGLVDSVNTKVNGAQAEGCSPI
                     ATAFREGRDFFKPVKPHTIAKSLAIGNPADGYYALKTAAESQGAMDAVTDDEIVEGIK
                     LLAQTEGIFAETAGGVTIGVLRKLVKQGRIQKNDVTVAYITGNGLKTQEAVVDSVGRP
                     FRIPPSLSHFIKTFAIEESA"
     gene            7153..7428
                     /locus_tag="PKM72_05385"
     CDS             7153..7428
                     /locus_tag="PKM72_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNP60251.1"
                     /translation="MIKVRIPTPLRPMTGGKSEVEIAGNTVSEIIDNLGSAHPGIKER
                     VYDEQGEVRRFINIYVNEEDIRFLTGKDTPLKDGDEVSIIPAIAGGA"
     gene            7425..7667
                     /locus_tag="PKM72_05390"
     CDS             7425..7667
                     /locus_tag="PKM72_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNP60252.1"
                     /translation="MMAKLRFHIRYPEDKIPSPILYEIGQEFQVVPSIRRADVRETTG
                     WMDVEFSGETDEIDRAIQGLRQKGCMVDPIELNVVE"
     gene            7682..8482
                     /gene="moeB"
                     /locus_tag="PKM72_05395"
     CDS             7682..8482
                     /gene="moeB"
                     /locus_tag="PKM72_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNP60253.1"
                     /translation="MEFTESQIQRYSRQIILSEVGGKGQKKLQDAKILVIGAGGLGSP
                     AALYLAAAGIGTLGLTDGDVVDLSNLQRQILHTTDRIGVPKVESGGTLLSALNPEITL
                     NLYPEHVSVTNILALIEAYDIVLDGSDNFSTRFLVNDACFFAKKTLISGSIFRFEGQL
                     VTIKPHEGFPCYRCLYPEPPPAGLVPNCQEAGVLGALAGTIGVLQANEAIKEVLGLGE
                     SLAKHLLLYDALDMTFRKVGRPKSPDCPLCGPHPTITKLVEQEVSCSI"
     gene            8487..8996
                     /locus_tag="PKM72_05400"
     CDS             8487..8996
                     /locus_tag="PKM72_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNP60254.1"
                     /translation="MLSIPSSIINNMIAHARELAPHECCGILSGTGSTITEYYRITNI
                     LAELSEEELSRFDRAKLSDLQRLSPEERADIAFQMDAREMAMAQRDIRSKNLDLLGFY
                     HSHTFSPARPSQTDITIAMEFESYRAKLNFPEPFHLIISLEHMEQPVVRAYRIQESKA
                     TEIPIQTMP"
     gene            9121..9471
                     /locus_tag="PKM72_05405"
     CDS             9121..9471
                     /locus_tag="PKM72_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60255.1"
                     /translation="MPNLHHLLAFLLGLIVFFQPGSQVYAEASTSLQKFNSPIMKIES
                     DKGVFLITTDSGIQWVQVEEEGKIQLKTLDVGDIIDVIVEMRPDPTPPLVKSWKLARS
                     GSSCKVFNGRTCSP"
     gene            complement(9749..10996)
                     /locus_tag="PKM72_05410"
     CDS             complement(9749..10996)
                     /locus_tag="PKM72_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNP60256.1"
                     /translation="MGLLVIPEVFRYSNTIMRRIVPKSLKASGRGEVLLQESVCNRLR
                     DIRKKQGISQVELAALVGLTRQAVYAIEANQYLPSTNVALRFARALKCRVEDIFILSN
                     QEEMVEAEFIGAPTALGHPTRVKLAYVGSRILARPMAELGDVLNFVMPADGVVVEQTK
                     RSTSPKRPYVRVQLLNSPEVIKKGILIAGCDPALFLAGEHVRKVNAMAGITNWTMGSA
                     NALRALQREEVHMAGLHLVDVKSGQSNVPYLKRHIPGQDFVGVRFASWVQGLLIQQGN
                     PKHIRTVEDFGQGGIRLVNREVGAGARFLLDTLLQKSGLTGEMLLGYDNEVPSHLEVA
                     RLIRDGIADVGIGVEAAARHFGLDFIPLREEQYDLIMRREFLTSHPVMSQFLDAMVSH
                     PFRREIESLGGYTVTEIGKILHW"
     gene            complement(11118..11798)
                     /locus_tag="PKM72_05415"
     CDS             complement(11118..11798)
                     /locus_tag="PKM72_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60257.1"
                     /translation="MPKKWGRVWPWCLLGGWLLAGCVAPPFYSYLVYENPTSFVRLEV
                     SPWVDTDVPQTLNEHPATMSRHQMIEALSGLRVREYRSGPIRWVRGLADPEPAFLKEE
                     IELLAPRLLDGLGLAVPQELVTFYISHPVNATKREVTSGGMYVRDGHLHVIISNHRTT
                     YEIPPAGLIYDRRYPLFSLAPLDVDLLYDSEETVMPKEKKFWETILGDEHSGEIVLDL
                     SRLSMMKM"
     gene            complement(11791..12312)
                     /locus_tag="PKM72_05420"
     CDS             complement(11791..12312)
                     /locus_tag="PKM72_05420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNP60258.1"
                     /translation="MSDATQKDQTITIHIQVNGDSWGDIHLRLFSDVAPNHVQNMLKL
                     AKEKFYDGTTFHRVIPNFMIQGGDPNSKEQDRSRHGMGGPGYRVDAEFSSTPHKRGTL
                     SMARSQDPNSAGSQFFICVADSSFLDGQYTVFGEVVSGMETVDRIVNAKRDANDNPID
                     RIEMTMSVPEANA"
     gene            complement(12338..13480)
                     /locus_tag="PKM72_05425"
     CDS             complement(12338..13480)
                     /locus_tag="PKM72_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60259.1"
                     /translation="MKKKAKRMGSGIRSTQGQKPLWNCLVGTVASLWLMGFSGLGDSR
                     PLLSSVYPVSNIPSLHPDDSENTHQGFAPSIQTLVVTPDGVVYAGSFGMGLFRSQDKG
                     KSWDALNRGLTDPFLLCLTVTPGKHIYAGTMRGGVYRLGLKGTTWESIGTGLHEAEVK
                     SFLVHGDAVYAGTGTGVYRWVEAEKQWVIVGAGLDRILVPGLAIMDDGKLFAATSGKG
                     LFHLETQQSSPAKWVDSQSIFVDPRERLPHRYLRVIAVNEAQHIFLGTQDGGIFRSTD
                     RGQSWLSLSRNLPNDSIRSIVPDHEDIIVATGNGIFRWKTDQQQWVGMNTGLTNLAIQ
                     SLTLSDAGELYAGTSSGAFRSQDGGAHWTNISQGLGVQLVPKGPYE"
     gene            complement(13464..14264)
                     /gene="tatC"
                     /locus_tag="PKM72_05430"
     CDS             complement(13464..14264)
                     /gene="tatC"
                     /locus_tag="PKM72_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNP60260.1"
                     /translation="MAAFSPTMAMNAVVHPLQRHIRDIKRRLLIVGATIMVFFVIAFS
                     YSSILIDWFKRPFEDDLIFYAPAEALFASIKISFMAAVIASVPIILYQFWKFIEPALL
                     QKEQRWAVPLFFLGLGFFLLGLAFCNMVILPLVIDFFVTFGMDRAITPELAVGTYVDF
                     NVKFLLAFGFAFEIPLVISLLSRAGVIQASVLMRFRKHAALVALILSAVITPDATMFT
                     MLLMAVPLILLYEIGIWGAKVFGRVPQSAGEKSQATEGDEGVDDEEKG"
     gene            complement(14303..14545)
                     /locus_tag="PKM72_05435"
     CDS             complement(14303..14545)
                     /locus_tag="PKM72_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNP60261.1"
                     /translation="MTDEQIAENLRSSNVEYQELEESHHRLDLELQQLLKHHVLTPQE
                     EILKKHLQKEKLGKKDRMAALIREHRASCDNANTPG"
     gene            complement(14593..15126)
                     /gene="rimI"
                     /locus_tag="PKM72_05440"
     CDS             complement(14593..15126)
                     /gene="rimI"
                     /locus_tag="PKM72_05440"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNP60262.1"
                     /translation="MGTDPFHNEAIRLATLADLDALVSLEESCFAVPWSRKSFAVELD
                     GNSFSRMLVIPGPKEQPEIPLLAYICIWVIFEEIRFLNLAVHPHFRGNGLAKQLILQA
                     LCIGSAQGCCRGMLEVRDSNQMAKKLYELFKFKEYGRRSSYYTNPSEDAILMILEPIS
                     GALSDKNDLGEIQVECK"
     gene            complement(15190..15885)
                     /gene="tsaB"
                     /locus_tag="PKM72_05445"
     CDS             complement(15190..15885)
                     /gene="tsaB"
                     /locus_tag="PKM72_05445"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNP60263.1"
                     /translation="MLFLALESATSHQSVAVFRDEQLLGSLDCESGQPLTPQLIPTID
                     RLLSSVSLHLSNLEGLVVSIGPGTFTGLRVGLATMTAFRMALQIPLVGVSTLEGLAWN
                     HPVTDLPLLSTIGIRQGVVYWALFRWENQQMVCVKESQIGDIIDVCGALSEPTIVLGD
                     GWMQNRTTLLSKGFSLLEAPSEVQWPSAKGTGMAGRVLLERGVLLPEGCTPQYIQPSY
                     AEISKTKSANPIK"
     gene            complement(15941..16723)
                     /locus_tag="PKM72_05450"
     CDS             complement(15941..16723)
                     /locus_tag="PKM72_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60264.1"
                     /translation="MKATSRWVLLPMVLLVGCAGILPESLRLLEDGQEQQVLQALRQK
                     ELTILTLRGLFHGSISGSGVPLSQNILGTVSYVRPDVLDLKGFIRMGVPVMDFHRKGN
                     EYELYFPAEGKVVTGKVDDTQERTAWDHTVMLSIRALDAVLGKISGGSSKEARVWKSN
                     ERYRIDLPENQSSATASQDDFTVRTWVDAQTMDLISIEYRRSFDDIVVSVECEDYREV
                     QVTTTEESLPVRLPYLVRATDYRPSGGSITLHFKEFIVNAAS"
     gene            complement(16751..18130)
                     /gene="radA"
                     /locus_tag="PKM72_05455"
     CDS             complement(16751..18130)
                     /gene="radA"
                     /locus_tag="PKM72_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNP60265.1"
                     /translation="MKAARPKTRFVCQECGHQGVRWSGRCPECAQWNSLREEVDRDLS
                     TLQQRSVAGGTPEAVPISSIVQTQEFRLQAGIEELNRVLGGGVVPGSVILIGGDPGIG
                     KTTLLLQTLASLGTARQVGLYVSGEESPQQIKMRADRIGIQSPNLYVAAATSLEEIFK
                     VAEQMNPGVIVVDSIQTVFTQELTSAPGSVSQVQEVGCRLMWFAKRTHVPIFIIGHVT
                     KEGVIAGPRLLEHIVDTVLYFEGDKGQSYRILRAVKNRFGPTNEIGVFEMKDEGLTEV
                     GNPSELFLTGRIGHGAGSVVVSSVEGSRPLLVELQALVAETTYPMPKRMAKGVEVNRV
                     SLLLAVLEKRLGLHFAGYDVYVNVVGGLRIDEPTVDVGIVCAVLSSFRELALDSRLVV
                     MGEVGLGGEVRPVQHADLRIREAMKLGFQRCVVPEPNLNQWKPVAGMEVVGIRDIGDI
                     WETVVSHAS"
     gene            complement(18243..19109)
                     /locus_tag="PKM72_05460"
     CDS             complement(18243..19109)
                     /locus_tag="PKM72_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60266.1"
                     /translation="MKQCDRHVLIFVRLSLALCLLVLGIGLTASLAQAANVSVTDLLT
                     SPGKTIQLQARLTNPEAEGEIGLADQPLEFIVHGRTVGQANTDAKGWARLDFAPSMRG
                     NLPIIVKVVTTSKVDAIQGKGVLLSWERRRPILLIDLAVLVEREVVTEPPPPELYNDP
                     GLILGNAHPAAASELGKLAEFYYNLVYLDLTGRGHLEDIQDWVREHQFPPGMIRILSK
                     TPTALTELIHDLKKEGWEKVSGGIGRTADFAEVLVQNRLQTVILPLPQTEDRFPRRAI
                     VLNDWSRVRRHL"
     gene            complement(19138..19377)
                     /locus_tag="PKM72_05465"
     CDS             complement(19138..19377)
                     /locus_tag="PKM72_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60267.1"
                     /translation="MNDREKTMHALGAATLSILPGIGHLYLGVKRGNIFLVCGILLMI
                     ISKLFWPTGWLIYMQFAILAGFDAFAFGKRGRGLF"
     gene            complement(19509..20123)
                     /locus_tag="PKM72_05470"
                     /pseudo
     CDS             complement(19509..20123)
                     /locus_tag="PKM72_05470"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
     gene            complement(20123..20518)
                     /locus_tag="PKM72_05475"
     CDS             complement(20123..20518)
                     /locus_tag="PKM72_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HNP60268.1"
                     /translation="MEFSPQKIKMTIGGIQVIAQLKPNRTAQAVVDALPVEVPVNQWG
                     EEFYCNMPGVKDYREVATTQVKIGDVAFWGMGGMLAVFFGRTPMSLGDDPVPADRVNI
                     IGKVIGDPRVLMEATGASLMKVERLPEGT"
BASE COUNT         5344 a         5543 c         4769 g         4953 t
ORIGIN      
        1 ttgacggttt ggctttcgcc ggtttttttt acctgcatct cgactttccc gcccatcatg
       61 gcgggattga tcccctgccc cgcaaacatt ttttccatca tggcacgtcg ttccggtggc
      121 atattcttta acatttcctg cattttttcc atggccgggt tgatctgttg accgagattg
      181 accatcgtgg ctttgtccat cgcccgccag gtgtgatctc catgattgat cagcagaatt
      241 tcctcctgat cgcctcgata aatgaccgtg gattgagtgt tcccggcatc atttatcgtc
      301 atcttcagtt ttttcccgtc gaccgttatc gcatcgtttc gagaaggcat ttctgcccga
      361 ttgggaaagg tcgtctcgac atgaaaaacc acgcctgcac aaaccgtcgt ctggatgccc
      421 agcaccaacc cgctcacaag accaagaacc ttcaccatat caacctcctt acgcgtgaca
      481 gatgggacag actgctgttt tgacatgctg gaaacgctga gacccgtcaa tctctcaaaa
      541 ttcaacccga ttcacaagtc tttttcgtgc agaggatccc tcatcgcccc tctcccacta
      601 tatttttggt cccaccaaag acccttttta atgaggggag ctaggaattg gtttaccttt
      661 cctggtaaaa gaactatgga caggaaacgc tcgttaggcg accaccccac ataacgacag
      721 gcagacatcc ctcaaaaaga gtcacaagta gtcgtgactt ttcacggttt cgtcatcatg
      781 gtcagggacc atatgctgcc cggcgcgaca ggaccagcgg gtcgcggggt aggacacggt
      841 aagaaggccc ggtctcttta agatgaggcc atccagccca gggaagccgg ttcaatcaga
      901 accgtccctt tctctcgtct ttaacgaaca tcacataccg acagaccggc tcaagtcggc
      961 ctatacacag atgaggacct catccagaaa ggttgcaaaa ccgtcatgaa acacgacacg
     1021 tccaacccac ccacagctcc tacgatgaag ggcttctcac ccggctttgc ggccctcggc
     1081 cttgaagcgg cgctgctgac cacgctcgaa gcgttaggtt atgaagagcc cacccctatc
     1141 caacgcgaag ccattccacc gcttttggcc ggaaaggacc tcctggggcg tgccgcaacc
     1201 ggaactggaa aaaccgctgc attcaccctt cccctcctcc agcgcctggc ccattcaccc
     1261 aagcaaccga acccatccgc ccttattctc gtgcccacac gcgaattggc tatacaagtc
     1321 tgcgaagctg ttcagcgtta cgggaaacaa cagcacatcg ctatcctcgc cgtctatggc
     1381 ggacaggcca tccgcccaca gcttatcgcg ttgaaacgcg gggtcgatat cgtcgtggcc
     1441 actccaggac gggcactgga tcatatccga cgcaaaaccc ttcagctcaa ggaccttcag
     1501 attgtggtgc tggatgaggc cgatgaaatg ctcaatatgg gtttcgccga agatctggat
     1561 gccattctga aacaaacgcc tgcgaccagg cagacagcac tgttttcagc gaccatgcct
     1621 ccacggattg cctccatcgc acgccagcat ttacgcaatc ccattgagat tcagatcata
     1681 aaggaaccgg tcaaggccgg ggtggcccct cgggtgcatc agaccgccta cctcgtggcg
     1741 agacctcaca aagtggctgc gctggccaga atcctggata tgtccggtcc aaaatccgca
     1801 ttggtctttt gccgaacacg cttggaggtg gatgagttga cctctatgct caacagccgg
     1861 gggtatcggg gagaaggcct gcatggcggt atgaaccagg ctcaacgcga tcgcgtgatg
     1921 caatccttcc gcgccgggaa aacggaattg cttgtcgcca cagacgtggc ggccagaggg
     1981 ctggatattc ctcacgtatc gcacgtggtg aattacgatg ttccgtcctc tcctgaagcc
     2041 tacgttcatc gtatcgggcg aaccggacgc gccggacgcg ccggtgaagc cattacactc
     2101 gtggaatcac gcgaacgctg gctgctgcaa aacattgagc gcctgaccaa atcaaaagtc
     2161 gacatgggaa cactcccgac tgtggccgac cttcaaacta agcggctgga acagattggc
     2221 gtgtccattc aagacattct caaagccaac gactttgaac aattccgagt cgtggtcgac
     2281 accctcaccg aaaaatttga ccaggcggat gtcgccgctg ccgcgattca attggcctac
     2341 gggtcaaaga cgggcgatcg agttgaagaa gacattcctg cgatacggga ccgggcatct
     2401 gacacaaccc agacgtccag acggccaagg cggggtccat cggattaccc ggatgctcct
     2461 agagttctca gaggcggagc tggtggtggc agaccggtcg gcagacggga ccgtcacagt
     2521 caatccacaa gtatggcgac cttgcacgta aacgccggtc gaatggcagg agtgcggaca
     2581 ggagatctgg tcggtgccat tgccaacgag gccgggatca gttcgaatgt gattggcccc
     2641 atcaaagtct ccgatcaatt ctctctggtt aaagtgccgg aagagcatgt ccatggcatc
     2701 atcaaggctc ttggccggac cagaattaaa gggcaaaaag tcaccatccg cctctataag
     2761 gaataaagcg gattcctgaa accgccaggg tgggaatttt cccaaccacg taggtatgcc
     2821 aaacatcact atcatgaaga aatagacatg agaaattaac acggccgttg tcaatatctc
     2881 tctgaatgtg aatcaatttt atgtctaatg gaatgacaat tcttgatgtg cgtgaccgga
     2941 tagccatggc ccgttcggtc acggttctta ccggtgcggg aatttccgca gatagcggcg
     3001 tgccgacgtt tcgaggcccc gaaggcttgt ggaaaaattt ccggcctgaa gaactggctt
     3061 caccggaggc ctttgcccgg gaccccaaac ttgtttggga atggtataac tggcgacgag
     3121 aattagtcgc aaccaaacac ccgaatgcgg cacatgaaac tctcgttgaa cttgaacgac
     3181 gaattgaaca cttctggctc attacgcaaa acgtcgatgg gcttcatgcc ctggccggct
     3241 cgcaacgtct ttcggaaatc catggcaata tctggaaagt gcgttgtaca caatgcaacc
     3301 ggatttcgtc taaccgcgat ctgcctctgg tctatccacc aacctgcacg gactgttcgg
     3361 ggttgttgcg cccccatatt gtgtggtttg gagagtcctt ggatcaggaa gatttgaatc
     3421 gcagttatca ggcattgcga acttgtgata ttcttctcat tattgggacc tcaggcgtgg
     3481 tctatccggc agcctctttt gctccagttg ccaaagatgg tggcgccctg gtggtcgaat
     3541 tgaatttgga tccgacgccc aattcctccg tggtggacgt gtcctttcag ggccgtgcca
     3601 aagatctggt gccgctcttg cgacaagtca cctaatcgca ccatttcttg actcccttcg
     3661 taggggtttg ttacgttttt tgtttaggga ttttcagtac ttacgtttgg agcacggcat
     3721 gcaattgacg atcaatggta aacccgaaac cctggaggtt tctacggtgc tagacgtctt
     3781 gaaaaccaaa gatattgacc cgcaactcgt tgctgtggaa ctgaatacgc agatggttga
     3841 tcaagagcaa ttgggaacga cttctcttaa ggatggcgat aaactcgaat ttttattttt
     3901 catgggcggt ggcgcgtgag tgctgcatat cttcgaaata ttacggatgg cattgggcat
     3961 actccgttag tgcgattaaa ccggctttcc ccagcaggag gagccacaat ttatgggaag
     4021 gcggagtttt acaatcctgg cggcagcgtc aaggatcgta tctgcctgaa tatgattgat
     4081 gaagcagaga acaagggtct gctcaagcct ggtggcacca ttgtggaacc gaccagcggc
     4141 aataccggca ttggattagc cctggtgtct gccgttcggg gctataaatt aattctggtt
     4201 atgccggagg ggatgagcct ggaacgggcc agcctgctct cctcctatgg cgcgcagttg
     4261 gtgttgactc ccgcccggga agggatgcga ggatcgatta aagaagcaga aagtattttg
     4321 gagcaaaatc ccgaatactt catgccgaac cagttttcta acccggctaa tccagccatt
     4381 catcgcaaga caacggcctt ggaaatctgg gaggccttgg agggcaaggt ggatgcattc
     4441 gtcgctgcgg taggaaccgg cggaacgatt accggatgcg gagagctgtt taaagaaaac
     4501 aatccttcgg tcaaggtgat tgcggtagaa ccaaccggct cccccgtatt atcgggagga
     4561 gaaccagggc cgcacagaat tcaagggatt ggcgctggct tcgttcctaa agttctgaat
     4621 cggtccctca tcgatcaaat tatgaccgtg acggatgaag aggcttacca aaccacgaag
     4681 caactagcca ggaaggaagg gctcctggtt ggaatatctg caggggcaaa tatatttgcc
     4741 gctcaaaagg tcgcggaagc cttaggcccg aataagaatg tcgtcaccat tctctgcgat
     4801 acaggcgaac gatatctaag cattgagaaa tattttaata tttaatgcaa ttccacggat
     4861 ccccttccct gaaggaagaa tttgaggagg acaacgtccc tccctcaaaa agataaggat
     4921 aactgagatg tgctctgcaa agggttagtg gagaatgatt ccatggccca ataattattt
     4981 ttgatcacgc tcacctcatt ctcctcggat agcgaggatg aaccaacatt aagcacgtaa
     5041 aaggtcagag gtcatatgag tttcacagaa gaccaaatta ctcgatatag ccgtcacatc
     5101 ttactgccgg aaataggcgg taaaggccag aaaaagctgt cacaagcgaa agtctttatt
     5161 gtcggcgccg gaggcttggg ctccccggtg gcctattacc ttgcggcagc cggcattggc
     5221 accattgggt tgattgatag tgatgtggtt gacctctcca accttcaacg acaaattctt
     5281 caccataccc ctgatgtggg aaggtctaag gtcgtttcgg ctaaggaaaa aattcaggcg
     5341 gtaaaccctg atgtccaggt caacatccat gaagaacgaa tgacttctcg caatgctcaa
     5401 gaattaatta accaatatga tgtcgtcatt gatggggtcg acaacttccc cgcaaaattt
     5461 ttgattaatg atgcctgtta cctggagaac aaacctcttg tgcatggagg aattctccgg
     5521 tttgaaggac gagtcttcac cattattcca aatcaatccg cctgttaccg ttgtgtgttc
     5581 aaaaatcccc cgccagcagg agttgtccct acttgtcagg aagccgggat catcggggtg
     5641 gttgccggac tcattggaac gattcaggcc acagaagcca ttaaactcat cctcaacatt
     5701 ggaacgcctt taacgaaccg tattctggat tttgacgcac gcaccactgc atttcgggaa
     5761 attcgcgtca aacggaatcc ctcatgtgcg ctttgcggcc caaatccttc gttgacggaa
     5821 cttatcgact atgaacagga agcgtgccaa cttcaaccat cggctacaac gctaaccaac
     5881 ggataaaccc cgccgtttaa aaggggagga tgatgctatg aaaaaaatgc tagctctagt
     5941 gtgtcgggag tgcgggaaag aatatcctac gcaggatatt catgtatgcg aattatgctt
     6001 tggcccactg gaagtcaaat acgattatgc cgtcatcaaa caggtcatga cgcgtgaaaa
     6061 aattacatcc ggtcctcgca gcctttggcg atatgtggat cttctccccg tggaaggcac
     6121 caatttcatc ggtcttcatg ccggatttac cccgctggtt catgccaaaa atctgggggc
     6181 cttcctcgga ctggacaacc tgtacattaa aaatgattgc gtcaatcatc cgacgttgtc
     6241 gtttaaggat cgcgtggtgg ccatcgcctt aactcgggcc agggaactgg gatttgaaac
     6301 cgttgcctgt gcctcaaccg gcaatctggc aaattcggta tcggcccatg cagcttcggc
     6361 aggaatgaaa tgctatgtgt tcattcccgg agacctggaa gccgcgaagg tgctgggcaa
     6421 tctgatttat cgtccgaatg tcgtggaaat tgaaggaaat tatgatgatg tcaatcgact
     6481 ctgcagcgaa atcgcgggag aacgtcactg ggcattcgtc aacatcaata tccgccccta
     6541 ttatgctgaa ggctcgaaaa cgttggcctt tgaaactgtc gagcaattag gttggcgggc
     6601 tcctgatcaa gtcgtggtgc ccatggcatc cggatcattg ctgacaaaaa tttggaaagg
     6661 cctcaaagaa tttgagaagt tgggcttggt cgattcagtg aacacgaaag tgaacggggc
     6721 gcaagcagaa ggctgctctc ccattgccac ggcatttcga gagggccgtg atttctttaa
     6781 accggtgaaa ccccacacca ttgccaaatc gctggccatt ggcaatccgg cggatgggta
     6841 ctacgcatta aaaaccgctg cggaaagcca aggggccatg gacgccgtca ccgatgatga
     6901 aatagtggaa ggcattaagc ttctggctca aacggaaggg atttttgctg aaactgcggg
     6961 gggggtcacc attggggtcc ttcgcaaatt agtgaaacaa gggcgcattc aaaaaaatga
     7021 tgtgaccgtg gcctatatta ccgggaacgg actgaaaact caggaagcgg tcgtcgattc
     7081 cgtcggacgc cccttccgca tcccacccag tctcagtcac tttatcaaga cttttgccat
     7141 agaggagtca gcatgattaa ggtcagaatc cccactccct tacgccccat gaccggggga
     7201 aaaagtgaag tggaaatagc ggggaacacg gtgtcggaaa taattgacaa cctgggctct
     7261 gcccatcccg gcattaagga acgcgtctat gatgaacagg gagaggttcg ccgattcatc
     7321 aatatctatg ttaatgagga agatattcgg tttttaaccg gcaaagacac cccactgaaa
     7381 gacggagatg aagtctccat cattcccgca atcgcaggag gtgcatgatg gccaaattac
     7441 gctttcacat tcgatatccc gaggataaaa ttccttctcc catcctgtat gaaatcgggc
     7501 aggaatttca ggtcgtgccc agtatccgac gggccgatgt ccgtgagacc accggatgga
     7561 tggatgtgga attttctgga gaaaccgatg aaattgaccg ggcgattcag ggtcttcgtc
     7621 aaaaaggctg catggttgat ccgattgaac tgaacgtcgt tgaataagga tttcgcttcc
     7681 catggaattt actgaatctc aaatccagcg ttacagccgt caaattattc tgtcggaagt
     7741 gggcggcaaa ggacagaaaa aattgcagga tgccaaaatc ctggtgattg gggcaggagg
     7801 ccttggttct ccggcagctt tgtatcttgc tgcggccgga atcggaacct taggtctgac
     7861 tgatggcgat gtcgtggacc tatccaatct tcaacggcaa attctccata caaccgaccg
     7921 tattggtgtt ccaaaggttg aatccggcgg gacattatta tcagccctca atcctgaaat
     7981 cactcttaat ctttaccctg aacatgtcag cgtcaccaat atccttgcct taattgaggc
     8041 ctacgatatc gttctagacg gatccgacaa tttctccacg cggtttctcg tcaatgatgc
     8101 ctgttttttt gcaaaaaaaa cgctgatttc agggagtatc tttcggtttg aagggcaatt
     8161 agtcacgatt aaaccccatg aaggctttcc ctgttatcgc tgtttatatc ctgagcctcc
     8221 accagccggc cttgtgccga attgccaaga agccggggta ttaggggcct tagccgggac
     8281 cattggtgtc ttgcaagcca acgaggctat taaggaagtc ctcggacttg gagaaagttt
     8341 ggcaaaacat cttcttctct acgatgccct tgatatgaca ttccgaaagg tcggtcgtcc
     8401 aaaatctcca gactgccctc tctgtggtcc ccatcccacc atcaccaaac ttgtggaaca
     8461 ggaagtttcc tgtagtatct aggtccatgc tttccattcc gtcaagtatt atcaacaaca
     8521 tgatagccca tgcgcgggaa ttggcacctc atgaatgttg tggcattctt tcaggaaccg
     8581 gaagcacaat caccgaatac tatcgcatta ccaatattct ggctgagtta tccgaagaag
     8641 aactttcacg ctttgaccga gctaaattgt ccgacctgca acgtttatcg ccggaggagc
     8701 gagcggatat cgcctttcag atggacgcga gagaaatggc catggctcag cgggacattc
     8761 gttcgaaaaa tctcgacctt ttaggatttt atcattccca cacatttagt ccggcaaggc
     8821 catctcaaac cgatatcacc atcgccatgg aatttgaaag ctatcgggcc aaactaaact
     8881 ttcctgaacc atttcacctg ataatttcct tagagcatat ggagcagccg gtggttcgag
     8941 cctaccgaat ccaagaatcc aaggccacag aaattcccat ccaaaccatg ccctagctca
     9001 ggccatcaca aacatgtctg caatttcctg atgcaacccc catagaaacc cccatagact
     9061 ttctgctaag aatattgcga taatgagaaa ggcttctcaa agtaacaaca ggagaaattt
     9121 atgccaaacc tccatcacct gcttgctttt ctgctagggt tgattgtttt ttttcagcct
     9181 gggtcacagg tgtatgccga agcttcaacc tcactccaga agttcaacag tcccattatg
     9241 aaaattgaat cggacaaagg cgtatttttg attacgactg attctggtat tcaatgggtt
     9301 caggttgaag aagaaggaaa gattcaactg aaaactttgg atgtgggaga tatcattgac
     9361 gtgatcgtcg aaatgcgacc agaccctact cccccattgg ttaaatcctg gaaactagcc
     9421 agaagtggtt cttcctgtaa agtgttcaat ggcaggacct gcagtcctta atactttctc
     9481 aacggtaaaa ttctaaccgc cacttttcca cgaactctct tgaggcctat ttcctcaatg
     9541 agccatagcc caacacacct gaacctggca gcttttccga atgagaaggt attgccttaa
     9601 cccctgtccc ctattgggaa atatggtaaa aactgagtac gtgatggtgg caatgcagcc
     9661 aatacatcaa attccatgag aaaacggtgg tatgttgcga ccgtgcccta tctcctgacc
     9721 tccccgagac attggaggag acacgaaatt accaatgaag aatttttccg atttcggtca
     9781 ccgtgtatcc cccaagtgat tcgatttccc gcctgaaggg atgactgacc atggcatcaa
     9841 gaaattgaga catcacgggg tgggatgtaa gaaattcgcg acgcataatc aggtcatact
     9901 gttcttctcg aagcggaata aaatcaagac caaaatgacg cgctgcggct tccactccaa
     9961 tgcccacgtc agctatgcca tctcgaatca gacgagccac ttcgagatga gacggcactt
    10021 cattgtcata ccccagtaac atctctccgg ttaatccaga tttttgaagg agcgtatcca
    10081 aaagaaatct cgcgccggct cctacttccc gattgacgag acgaatcccg ccctggccaa
    10141 aatcttcaac agtacgaata tgtttgggat ttccctgctg aattaagaga ccttgaaccc
    10201 aggaagcaaa tcgcactccg acaaaatcct gcccgggaat atgtcgtttc aggtaaggaa
    10261 cattactttg tccggatttc acatccacaa gatgcaagcc ggccatgtga acttcttccc
    10321 gttgaagcgc acggagcgcg ttcgcgcttc ccatggtcca attcgtgatt cccgccatgg
    10381 cgtttacctt acggacatgc tccccggcta gaaataacgc cgggtcgcat ccggcaatga
    10441 gaatcccctt tttgatgact tccggagaat tcagtaattg aacgcgtaca tatggccttt
    10501 tgggactggt cgatctcttg gtctgctcca caaccacacc atctgctggc atcacaaaat
    10561 ttaacacatc acccagttca gccatcggcc tggccagtat ccttgatcca acgtatgcca
    10621 gcttaaccct tgtcgggtgc cccaacgccg ttggggcacc aatgaattca gcttcgacca
    10681 tttcttcttg atttgaaagg ataaaaatat cctcaacccg gcacttcaac gcacgagcga
    10741 accgtaaagc aacattggtg cttggcaaat attgattggc ttcaatggca tagaccgctt
    10801 gtcgtgtcag acccaccagg gccgctaact ccacctggga aattccctgt ttttttcgta
    10861 tatctcgcaa gcgattacac acggattctt gaagcaagac ctccccccgg ccagaagctt
    10921 tcagactttt agggacaatt cttctcataa tcgtattgga ataacgaaat acctccggga
    10981 tgaccaatag tcccatagcc tacgaaagac tctttcatca tcgttggtct tctctatgca
    11041 gaacctcatt aaggaaggct tatcgttgtc tctcggcctc tttacatccc ctaccggtac
    11101 aacccaggat tagacgttta cattttcatc atcgacaatc gggaaagatc gagaacgatc
    11161 tcaccgctgt gctcatcccc caaaatagtt tcccaaaact ttttctcttt tggcatcacc
    11221 gtctcttctg aatcataaag taaatctaca tcaaggggag ccagagaaaa caacggatac
    11281 cgccgatcat agatcagccc tgcaggggga atttcatacg ttgttcggtg attactgatg
    11341 atgacgtgga gatgcccatc tctgacatac atccctcccg aggtcacttc ccgtttcgta
    11401 gcattcaccg gatgactgat gtaaaacgta accagttcct ggggaacagc taaccctagt
    11461 ccatcgagta aacgtggggc taacaattct atttcctcct tcaagaacgc cggctcagga
    11521 tccgcaaggc cacggaccca tcggatgggg cctgaacgat actcacgcac ccgcaatccg
    11581 ctcagggcct cgatcatttg atgtcgagac atggtggcag gatgctcgtt caatgtctga
    11641 ggaacatcag tgtcgaccca aggggaaacc tcaagtcgaa caaacgatgt gggattttcg
    11701 tagaccaggt aggaatagaa cggtggagca acacaacccg ccaatagcca tccaccaagg
    11761 agacaccaag gccagacccg cccccacttc ttaggcattc gcttcaggga cactcatggt
    11821 catttcaatc cgatcaatag gattatcatt ggcatcccgc ttagcattga cgattcgatc
    11881 aactgtctcc attccactca ccacctcacc aaacacagta tattgaccat ctaaaaaact
    11941 cgaatctgct acacaaataa aaaattggga acccgcgcta ttaggatctt gcgatctcgc
    12001 catcgatagc gtccctcgct tatgaggcgt ggagctaaat tcagcatcga ctcgatagcc
    12061 cggcccaccc attccatggc gtgaacgatc ctgctcctta ctattgggat ctcccccttg
    12121 aatcataaag ttgggaatca cccgatgaaa cgtcgtgccg tcataaaact tttctttggc
    12181 taatttcagc atattctgaa catgattggg cgcgacatcc gaaaaaagcc ttaagtggat
    12241 atcaccccag gaatcgccgt tcacctgaat atggatagtg attgtctgat ctttctgagt
    12301 tgcgtcgctc atctcccgtc ctttccgtta cctggattta ttcataaggg cctttgggaa
    12361 ccaactggac acccaaccct tggctaatat tcgtccaatg cgcacccccg tcttgacttc
    12421 gaaatgctcc actactggtt ccggcatata actccccggc atccgatagc gtgaggcttt
    12481 gaatagccag gttagtcaac ccggtgttca tgcccaccca ttgttgctga tcagtcttcc
    12541 atcggaaaat tccatttcca gtggccacaa taatatcttc atgatcgggg acaatgcttc
    12601 taattgaatc attggggaga ttccggctga gtgacaacca ggattgacca cgatccgtac
    12661 tccggaagat ccctccatcc tgtgttccta aaaaaatgtg ttgcgcctca ttgacggcaa
    12721 tgactcgaag atagcgatgt ggcaaccgtt cccgtggatc gacaaatatg gattgggagt
    12781 ccacccattt agcgggagaa gactgttgag tctccagatg aaaaagccct ttgccagaag
    12841 tcgcggcgaa aagtttccca tcatccatga tggccagacc aggaaccaga attcgatcca
    12901 atccggctcc gacgatgacc cactgttttt cggcttctac ccaccggtac accccggttc
    12961 ctgttcccgc atatacggca tccccgtgaa ccaggaatga tttgacttcc gcctcatgta
    13021 atcctgttcc aatggactcc caagtcgtac ccttgagccc aagacgatag accccaccac
    13081 gcatcgtccc ggcatagatg tgcttccctg gagtcaccgt aagacacagt aaaaatgggt
    13141 ccgtcaaacc tcgattcaac gcgtcccagg atttcccctt atcctggctc cgaaacagcc
    13201 ccattccaaa ggatcccgca tagaccactc cgtccggagt taccacaagg gtttgaatac
    13261 tcggggcaaa cccttggtgg gtattttcag agtcatcagg atgaagagag ggaatattcg
    13321 agaccggata taccgacgat aacagaggac gactgtctcc aagtccggaa aaccccataa
    13381 gccagagcga agcaactgtg cccacaagac aattccatag aggtttttgt ccttgtgtcg
    13441 atcggatacc tgatcccatc cgtttagcct ttttcttcat cgtccacgcc ctcatccccc
    13501 tcagttgctt gactcttttc ccctgccgat tgaggaacac gcccaaagac ctttgcgccc
    13561 caaatgccta tctcatacag taggatcaag ggaacagcca ttaataacat ggtgaacatt
    13621 gtcgcatccg gcgtaatcac ggccgaaaga atcaacgcca ccagagcggc atgtttgcgg
    13681 aaccgcatga ggacagacgc ctgaatgact cctgcacgag acagaagaga aatcaccagt
    13741 ggaatttcga acgcaaagcc aaacgccagg agaaacttga cgttaaagtc gacataagtt
    13801 ccgacggcta attccggcgt aatggctcga tccatgccaa acgtcacaaa aaagtcgatg
    13861 accaacggga ggatcaccat attgcaaaaa gccaacccca agaggaaaaa ccccaacccg
    13921 aggaagaaga gcggcaccgc ccatcgttgc tctttctgaa gcaacgccgg ctcaataaat
    13981 ttccaaaatt ggtacagaat gatagggaca ctggcaatca ccgccgccat aaaagaaatt
    14041 tttatggagg caaataatgc ctctgccggg gcgtaaaaaa tcagatcatc ttcaaagggc
    14101 cgcttgaacc aatcgatcag aatggaagag tatgaaaacg ctataacaaa aaacaccatg
    14161 atcgtggctc ccacgatcaa taatcggcgt tttatatcac gaatgtggcg ctgaagagga
    14221 tgcaccaccg cattcatggc catggtaggg gaaaaggcag ccattagaaa gtggggctta
    14281 gcgtgcgaag cagccgttcg gattatccag gtgtgttggc attatcacat gaggcacgat
    14341 gttcacgaat cagggcagcc attcgatctt tcttgccaag tttttctttc tgtaaatgtt
    14401 tttttagaat ttcttcctga ggagtcaaca catgatgttt taataactgc tggagttcca
    14461 gatccaaccg gtgatgggat tcctccaact cctgatactc cacattggat gaccgcaaat
    14521 tttcggcgat ttgttcgtcc gtcactttcg gtgccctccc gtgttagtag tgattgaacc
    14581 ggtaagcgtc tcctatttgc attcgacctg aatttcccct aaatcatttt tatccgacaa
    14641 tgcccctgaa atcggttcca gaatcataag gatagcatcc tcgctaggat tcgtatagta
    14701 ggaactcctt ctgccatatt ccttaaattt aaagagctca tagagctttt tggccatctg
    14761 attggagtcc cgaacctcca acatcccccg acaacatccc tgagcactcc cgatgcataa
    14821 tgcttgtaga attaactgct tagccagtcc atttccacga aaatgagggt gaaccgccag
    14881 atttaaaaag cggatttctt caaagatcac ccatatgcag atgtaggcca gcaagggaat
    14941 ttccggctgt tcctttggcc ctggaatgac aagcattcgg ctgaaagaat ttccgtccaa
    15001 ttcgactgca aagctttttc tcgaccacgg gacagcaaag caggactctt ccagactcac
    15061 caaagcatcc aaatcagcca atgtggctaa tcgaatggcc tcattgtgaa atggatcagt
    15121 acccacgagc cggtcctctg tgaaaatgct tcactcctct atgtcgcacg aacagatacc
    15181 caagaagtct cacttgatgg gattcgcgct ttttgttttt gatatctctg catacgatgg
    15241 ctgaatatac tgtggggtgc aaccttcagg aaggagaacg cctcgttcca gcagtacccg
    15301 tcctgccatt ccggtccctt tggccgatgg ccattgcacc tcggaaggag cttcgagaag
    15361 agagaatcct tttgaaagga gagtcgtccg gttctgcatc cacccatctc cgagcacaat
    15421 ggtgggttcg gacagggcac cgcagacatc aatgatatcc ccgatttgag actctttcac
    15481 gcataccatc tgttgatttt cccaacggaa cagtgcccaa tagacaactc cctggcgaat
    15541 accgatagtg ctgagcagag ggagatcggt aacaggatga ttccaggcaa gcccttcgag
    15601 tgtggaaacc ccaaccaaag gaatctgtaa agccatccga aaggccgtca tggtcgccag
    15661 gcccactcgt aatccggtga aagtccccgg acctatggaa accaccaaac cttccagatt
    15721 tgataaatgc aaagagaccg aggataacag ccgatcgatt gtggggatga gttgcggggt
    15781 cagaggttgg cctgactcac agtccagact acctaacaac tgttcgtcgc gaaagacggc
    15841 cacactctga tgagaggtgg cggactccag agctaaaaac agcatagagc ttcttgactc
    15901 ccgacttgct gattagtggt ggcttgacgc ataaggacaa ctacgaggcc gcattgacga
    15961 taaattcctt gaagtgcagg gtaatggatc ctccactggg tcgataatct gtcgcccgca
    16021 caagataagg caatcgaaca ggtaaactct cttcggtagt ggtcacttgc acctctcgat
    16081 aatcttcaca ttccacggac acgacgatat catcaaacga gcgtcgatat tcaatagata
    16141 tcaggtccat ggtctgggca tccacccagg tcctgacggt gaaatcgtct tgagatgccg
    16201 tggctgagga ttggttttca ggaagatcaa tgcgataacg ttcgttgctt ttccacacac
    16261 gggcctcttt acttgatccc ccggaaatct ttcctaacac ggcatccagg gcacgaatac
    16321 tcagcatcac ggtgtgatcc catgctgtgc gctcctgggt atcatcaact tttccggtga
    16381 ccaccttacc ttcagcggga aaatacagtt catactcgtt ccccttgcga tgaaaatcca
    16441 tcacgggcac gcccattcga ataaaccctt tcagatccag aacatcggga cgcacgtacg
    16501 aaaccgttcc aagaatattt tgagaaagag gaacgccgga accggaaatt gatccgtgaa
    16561 ataatcctcg tagggtgaga atcgtcaatt ccttttgacg caaggcttga agtacctgtt
    16621 gctcttgccc gtcttcgagc agtcgcaacg attctggtaa tattccggca catcctacga
    16681 gcaacaccat cggcaataga acccatcgac ttgtagcctt catcgtatac agtccgcctg
    16741 caggtaccat ttatgaggca tgactgacaa ccgtttccca aatgtcgcca atgtcacgaa
    16801 tccccacgac ttccatgcca gccacaggtt tccattggtt cagattcggt tcaggcacca
    16861 cacaacgctg aaaccccaat ttcatcgcct cacggatgcg caggtccgca tgctgtaccg
    16921 gccgaacttc tccgcctaac ccgacttctc ccatcacaac caatcgcgag tccagtgcca
    16981 actctcgaaa gctcgaaagc acagcacata cgatgcccac gtcaaccgtt ggctcatcga
    17041 tccgaagccc ccccaccaca tttacgtaca catcgtatcc ggcaaagtgc aacccgagtc
    17101 gcttttccag gacggccaac aacaacgaca cccgattcac ttctacacct ttggccatac
    17161 gtttcggcat agggtaggtc gtctccgcta ccagagcctg caattccact aataaaggcc
    17221 gagagccttc cacgctggac acgaccacag acccggcgcc atgtccgatt cgccccgtca
    17281 aaaatagttc tgagggattc ccaacctcgg tcagtccctc atccttcatt tcaaagaccc
    17341 cgatttcatt ggtcggcccg aaccgatttt tcaccgcccg aagaattcga tagctctgtc
    17401 ctttatctcc ttcgaaatac aacaccgtat cgacaatatg ctccagaagc cttggcccgg
    17461 caatcactcc ttctttcgtc acatgcccga taataaaaat agggacatgc gtccgcttgg
    17521 caaaccacat cagacgacag cccacctcct gaacctggct gacactaccc ggcgctgatg
    17581 tgagctcctg ggtaaagact gtttggatgg aatccacgac gatgacaccg ggattcatct
    17641 gctcagccac tttgaaaatt tcttccaggg atgtcgcggc agcgacatac aggttgggcg
    17701 attgaatacc tatgcgatcc gcccgcattt taatttgctg aggggattct tctcccgata
    17761 catacaaccc gacctgtctg gccgttccca aggaggcgag ggtttggagc aggagtgtcg
    17821 ttttaccaat tccaggatca cctccaatca agataaccga tcccggaacc actcccccac
    17881 caagcactcg attcaattct tcaatgccag cctgaagacg aaactcctga gtctgcacga
    17941 tggagctgat aggaacagcc tctggtgttc ccccagcgac acttcgctgt tgaagagttg
    18001 aaagatctcg atcaacctct tcccgtaagg aattccattg cgcgcattcc ggacaccggc
    18061 cgctccagcg gacgccttga tgtccacatt cctgacacac aaaacgggtt ttaggtcgtg
    18121 cggctttcaa cggagcatct cccactacga tctatcacat gagcagaaag ttatatgcat
    18181 cgagaaatat aaaccttcct caccggatca ttcatcgccc ggctctttgc tcactgtcgt
    18241 catcaaagat gccgtcggac acgtgaccaa tcattgagga caatggccct gcgtggaaac
    18301 cggtcctctg tctgtggcaa gggcaaaatc acggtttgca acctattttg aacgagaact
    18361 tccgcaaaat ctgccgtgcg cccaatgcca ccactcactt tttcccaacc ctcttttttt
    18421 agatcgtgta taagttcggt caatgccgtg ggcgtcttcg aaaggatcct aatcattcct
    18481 ggagggaatt gatgctcccg cacccaatcc tgaatatctt caagatgccc tcgcccggtc
    18541 aagtccagat acacaaggtt gtaatagaat tcagctagct tgcctaattc cgaagccgct
    18601 gcaggatggg catttcctaa tatgagcccg ggatcattat aaagttcagg cggaggcggt
    18661 tccgtgacca cctctctttc cactaacacc gccaggtcaa tcagcaatat gggacggcgc
    18721 cgttcccagg ataagaggac ccctttccct tggatggcat ccaccttcga tgtcgtcact
    18781 actttcacaa tgatcggtaa attcccccgc atactgggag caaagtcaag ccgtgcccac
    18841 cctttcgcgt cggtattcgc ctgcccaact gtccggccat gaacaataaa ttccaaaggc
    18901 tgatcggcca aaccgatttc cccttctgcc tcaggattgg ttaagcgcgc ctgtaattga
    18961 atggtctttc ctggagatgt gagtaaatcg gtcactgaca cattcgcggc ctgagccagc
    19021 gaggccgtca atcctatccc gagcaccaat aggcacagag ccagacttaa tcggacaaag
    19081 ataagcacgt gacgatcgca ttgcttcatt ctacgatctc catatacctg gcagatatca
    19141 aaacaatcct ctcccgcgtt tgccaaatgc aaacgcatcg aagcctgcca gaatggcaaa
    19201 ttgcatgtaa attaaccatc ctgtcggcca gaaaagcttg gaaatgatca ttaaaagaat
    19261 cccacagacc aagaaaatat tcccgcgttt gacacctaag tatagatggc cgatccccgg
    19321 taaaatggag agagtggcgg ctccaagagc atgcatcgtc ttttcacgat cattcatcag
    19381 ggtgacccta ccaaaccata ccattggcca tctggagagt tccagggata agaatcaggg
    19441 ctaaaaaagg attggggcac actcacagga atttaaaaat ctggagggag ggggaaaaga
    19501 gcaattcttc agcttaggtt ccgcttacgt aattcttcct ggtaggtttt ttgataaagg
    19561 acatcccact cttgggaacc ttccggaacc ggccgggaat acgattgcaa tcgacttcga
    19621 accttttgaa ccaactcctc ttcagctttc atgtgctcgg ccaacacacg cttcacttca
    19681 cgaagggcgt gagcagtatc gccgatcatc ttcacctcgg acatttctgt gacattggtc
    19741 agaatcacat gtgcaagatg tgtctgtttt tcgtcgctga ggatgggagt attcatcatc
    19801 aatccagtcg ttcaggcatt aatcacattc ttccgtctgc cctataaaat gaccccgcgg
    19861 tctttgatga gcttttgttt caccatcgta aacattttct gataatccgc tcgaccttcc
    19921 gcaaactccc gttcaaattt tttcaacatt tcccggacat ccgcattcaa ccgatcttcc
    19981 acctgcaact cggatacaat tgctgattcc aatttttgaa tgaccacttc agattttccg
    20041 tgaatttcca acaatccact cgactgtaat cgttcaatga ctgagacggc catctgatgc
    20101 acgcgaactt tattgagtcg catcaggttc cttcaggaag ccgttccact ttcatgaggg
    20161 aagcccctgt ggcctccatc aacactctcg gatcacctat cacttttcca ataatattca
    20221 cccgatcagc cggaaccgga tcatcgccaa gactcatggg agtacgccca aaaaaaacgg
    20281 ccaacattcc ccccataccc caaaacgcca catcaccgat tttcacctgt gtggtggcca
    20341 cctcccgata atccttgaca cccggcatgt tgcaataaaa ctcttctccc cattgattca
    20401 cgggcacctc aacaggcaga gcgtcaacca cagcctgtgc ggttcgattc ggcttcagtt
    20461 gtgcaataac ttgtatccca ccgatggtca ttttgatttt ctgtggactg aattccatat
    20521 attctctcta aggtttcgaa tgaaatacga tctaaagatt gcttcgaatg tagcttgtcc
    20581 tcttggtgaa atcaaggtta gaattaagc
//
LOCUS       DAORAQ010000037        20453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_477_length_20453_cov_61.630601, whole genome shotgun sequence.
ACCESSION   DAORAQ010000037 DAORAQ010000000
VERSION     DAORAQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 20453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20453
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_20453_cov_61.630601"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKM72_05480"
     CDS             <1..281
                     /locus_tag="PKM72_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60269.1"
                     /translation="PMPIFLVENDCPPFSLVKDGCLRTTEITCSGPSVFSVESSLSLR
                     ESRLGRIFYKDGWGGERRCLKHILMNAHWVEAQYAEKWYSKEREFPPV"
     gene            complement(522..1310)
                     /locus_tag="PKM72_05485"
     CDS             complement(522..1310)
                     /locus_tag="PKM72_05485"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNP60270.1"
                     /translation="MTDYRQELSIIRQAMLLASDRLHRIQYEGLAVQIKPDGSPVTNA
                     DLEVNRIIQEALFAAYPDDGWLSEESPDNTLRLKKNRVWILDPIDGTKPFIKSLPQFA
                     ISLALIDHGQASIGIIFNPATREYFCAVRGEPATVNGCPIHVPQIPGHRFSFLVNTGP
                     IARSTIHTWRETANCRSLMGSIAYSLALVAAGQIDGVINIGTQNEWDIAAALLLVQTA
                     GGVVVDRDMKPIQCNQPHPTINGIIAARPDAMPVIQQFLASLPA"
     gene            1534..1764
                     /locus_tag="PKM72_05490"
     CDS             1534..1764
                     /locus_tag="PKM72_05490"
                     /inference="COORDINATES: protein motif:HMM:NF022644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2835 domain-containing protein"
                     /protein_id="HNP60271.1"
                     /translation="MQTVTVRLKISPERFKAYYHGAVEYVVAPSIDGRTIQFPARVLR
                     PFVSHQGIQGTFEITFDANLKFHSIRLAQDDR"
     gene            1761..2213
                     /locus_tag="PKM72_05495"
     CDS             1761..2213
                     /locus_tag="PKM72_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNP60272.1"
                     /translation="MSATSMIGDEYSLVGLFLSAFLSSTVLPGSSEVVFVFFASQGHF
                     PAWSLLATATAGNTLGGMSSWALGWLIGWWYPFHGLTQPAQHLAVERVRKWGSPVLLL
                     SWVPFIGDPLCIAAGWLRVGWVRALVCIGIGKGCRYAVLFALLPTSGG"
     gene            2368..3486
                     /locus_tag="PKM72_05500"
     CDS             2368..3486
                     /locus_tag="PKM72_05500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNP60273.1"
                     /translation="MNVPLLDLKTQYASIKQDIQVALDKVCAEQSFILGTHVQNLEQT
                     LASFIGTDHAIGVASGSDALLLSLMEVGIEPGDRVVTVPFTFFASAGVISRLHARPVF
                     VDVMPDTFNLDPSRLADSFTSEVKAILPVHLFGQCADMESIVQIADEKGIPVIEDACQ
                     AIGAARNGVQAGAFGRTGCFSFFPSKNLGGFGDGGVITTRDPLVAERLQLLRVHGSRS
                     EYHHHFIGMNSRLDALQAAILQVKFQHLAEWTAKRQAHAASYLKLFQTCGLDERVTVP
                     VVSSGNTHVYNQFTIRTPRRDELSTYLTHHGIGNRIYYPVPLHLQECYQDLGYHKGDF
                     PVSEQLSQEVLSLPIYPELTADQLQYVVDTIKSFFDCR"
     gene            complement(3536..3940)
                     /gene="queF"
                     /locus_tag="PKM72_05505"
     CDS             complement(3536..3940)
                     /gene="queF"
                     /locus_tag="PKM72_05505"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNP60274.1"
                     /translation="MKKPHASSRLRYSERHAKSGIAEKLPRIETWPNQYPGYEITIEI
                     PEYTAICPKTGLPDFGTIRLTYVPDKSCLELKSLKMYIHAYRNLGIFYENAVNKILAD
                     FVKACRPVSAIVTGEFTARGGLRSVIQARYPQ"
     gene            4193..4705
                     /locus_tag="PKM72_05510"
     CDS             4193..4705
                     /locus_tag="PKM72_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNP60275.1"
                     /translation="MSSITMLEADDFVKGLQGIGFDFFTGVPDTILGGIIAHLTEEQL
                     YTPAVREDEAVAMAAGAYLGGKIPVVLMQNSGLGNALNVLASLNLIYQIPCLLLVSWR
                     GFEGKDAPEHLVMGETMTTLLDTVRIPHRTLSQKTIVDDLKWTATTFMEKRIPVALLL
                     KKGIVKTIQP"
     gene            4857..5438
                     /locus_tag="PKM72_05515"
     CDS             4857..5438
                     /locus_tag="PKM72_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNP60276.1"
                     /translation="MIGPEEGVMQSRAQAMAAILEIMTDQLLVVCNGFPSREACKLRD
                     RAQNFYMIGSMGATAAIGLGLALAQPEKTVVVFDGDGNVLMSLGTLATISALRPKNFI
                     HVVFDNEVYGTTGNQPTYSRVVGLDKMAKAAGYKNVERVWEREDIVYEFKAMLGDDGP
                     NFLLIKVNEQLEEADRIALGPAELTKRFKGSVT"
     gene            5508..6596
                     /locus_tag="PKM72_05520"
     CDS             5508..6596
                     /locus_tag="PKM72_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNP60277.1"
                     /translation="MILFNPGPVNVSERVRQALLQPDVCHRESEFSELLSDIRQKLLK
                     VFVPGNEDEYTAIVLTGSGTAAVEAALMSSIPTGKRAMVINNGVYGQRMSSMLATHRL
                     QTPDLKYDWGVAPDPQAVDLALKQHPEVHTVAMVHHETTLGLINPVKEIAEIVDRYGR
                     VFMVDSVSGLGGEALDIAGSKLYMVAGAAQKCIQGFPGAAFVLVRKGFMERVMKYPKR
                     SWYLNLANYYEEQERGTIPFTPAVQVYYAFREALNELLEEGLENRIQRFQGYATTIRT
                     RLDEWGVKAVLDPAIQGNTLTAFYLPEGCTYQRLHDELKQAGYVIYAGQGQLEQKIFR
                     VANIGALTNQDIEGFLSAFQSIVSGARA"
     gene            6593..7354
                     /locus_tag="PKM72_05525"
     CDS             6593..7354
                     /locus_tag="PKM72_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HNP60278.1"
                     /translation="MKAIIFAAGVGKRLQGVTQGRPKCLVDIGGRTLLSRHVEYLGRL
                     GVSQVVLVVGYAQDHIRKAMAAESFAQEIRWVVNEQFTRGSMTSLWAARSEMDDDVVL
                     MDADVLYAPSILSRLVHSSFPTALLMDETVKQESEECMIAAKTARVLTLSKTLPSDYD
                     EAGEGVGFLKVQQQDIPALLQSVRAYVDAGALDMEYEDTLKGFFEKVPVGYEKIGGLP
                     WIEIDFPEDIDRAVSEILPAVIKLEENTRATRLTP"
     gene            7365..8849
                     /locus_tag="PKM72_05530"
     CDS             7365..8849
                     /locus_tag="PKM72_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNP60279.1"
                     /translation="MDGTLKQEATHAVDTAILLTSVGVFNSGCAGTGDAVPSPLTRVG
                     GMTLFQRAVFTLQRGGISQIWVLAGPEEQALRQLLREDNRIQAAVRWLPVREFPPHDP
                     QTWEALAEEINGACMIVGCHTVYSPTLIQRLRAEGSQGKAVVVVGHPEEGHHRGNPGV
                     AFRPEGIEGRSTSTVVFHDQAVSQTSQSKTDSQTSERSRLPLVGDLLVLPARLLGISG
                     VLHANGTNPLRLALEQAAVEGTIQTIGGASHWFRDVRGPKGPQLAERTLLQALQTLKG
                     GLDGLVDRYVNRKCSGLLTQGFLRLGWSPNMITMLSMVVGLVAAGLFVPGSWKLAIFG
                     GLILQLSVIIDCCDGEVARLTFSESKFGQELDIWADNVVHIVLFAAIACGAFLHGPWE
                     HTHLPLLLGASAVLANVVSLLLVNHARHLRSRPREPRQFTEQEQANIEFMLGNVANRD
                     FSIIVLLGAGFGLLHWFLALAAIGSWLFVMSMAWMLRRSLISRA"
     gene            8842..9852
                     /locus_tag="PKM72_05535"
     CDS             8842..9852
                     /locus_tag="PKM72_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HNP60280.1"
                     /translation="MLKLLFLVVGLTALVGIILHIGLEPIQHTVSHLGLLHLLLILIP
                     MVFVYGLEALGWQFTLGFHARKVGFMRLFAIRMAGETVNVTTPTAYVGGEPLKAYLLK
                     RYGVPMVEGLASVITAKTTMTFAQVLFILLGLALAFWILGDSGHYWVAMLVSVGVLAF
                     GVGLFVLLQRHGLGMGCLGLLRACGIRFTFLEKREAQLQELDGTIRKFYSQHRETFYA
                     ALAVFFLAWMMETLEVYAILYFLDVSIDVWVAISIAALTVLIKGGTFFIPGSLGAQEG
                     GYTLLLMTFGYSEVTGITFALIRRMREILWIGFGLVCLMLLKGHDGGSDQIHSMERSG
                     DK"
     gene            9952..10383
                     /locus_tag="PKM72_05540"
     CDS             9952..10383
                     /locus_tag="PKM72_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60281.1"
                     /translation="MDIELGTNTLRNTDGVFIAHGKEQLRIEWLEEEKKLALSMGVFM
                     PTGTEVAKLQRNAWEHNSGDRFVLTELPDRVKVEDTTLKTLVLEIHKKSHEAVAIPSA
                     KFYTSKGTLSEISLEWWRVGNKMELAGIDMDLEGGSVEFPE"
     gene            complement(10719..11654)
                     /locus_tag="PKM72_05545"
     CDS             complement(10719..11654)
                     /locus_tag="PKM72_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60282.1"
                     /translation="MRCRKCPKRAVLGLPRHNTAFCGDCLTEFVRTQVQRAIKAQRMF
                     SPEDRILVAVSGGKDSLTLWEILLKLGYRVDALYVDLGIPGYSSRSKEKVEQFAKVVA
                     EPCGSQLTIHTVEEDAGAGIKELANMIKRPTCSACGTIKRYQFNRVAWQNHYNVMATG
                     HNLDDEAARLLGNVLQWQEEYLQKQSPSLPASVEGFAKKVKPLYRMTEREIAAYAVVN
                     RIDYLVEECPMAKGAKMLVYKDALNRLEAESPGTKQRFYWGFLDRQEKSSSVAPSMSE
                     IDQTTLQPCTVCSQPTTAGTCSFCRMMARAKTAVK"
     gene            complement(11659..11853)
                     /locus_tag="PKM72_05550"
     CDS             complement(11659..11853)
                     /locus_tag="PKM72_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiS"
                     /protein_id="HNP60283.1"
                     /translation="MKVHLSHPTRDVVLQGPKRVADIFKELNLIPEAFLIIRGQDLMT
                     EDETVADGDSIEIRPVISGG"
     gene            12103..14193
                     /gene="tkt"
                     /locus_tag="PKM72_05555"
     CDS             12103..14193
                     /gene="tkt"
                     /locus_tag="PKM72_05555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNP60284.1"
                     /translation="MKPACDSLDQLCINTIRTLSMDAVQAANSGHPGTPMALAPVAYW
                     LWNRILRSDPDDPIWPNRDRFILSAGHASMLLYSLLHLCGVKSVNGQYEHLGEPSVTL
                     EDIKRFRQLESKCPGHPEYRWTSGIETTTGPLGQGVATSVGMAIAQQWMAGYFNRPDF
                     EMFNYNVYSICGDGCLMEGVSSEAASLAGHLKLSNLCWIYDNNKITIEGPTDLAFSED
                     VATRFIAYGWNVTRVGDANDLNMLDRAFGTFLKELERPTLIIVDSHIGYGAPNKQDTH
                     SAHGEPLGEEEIRLAKRHYGWPEDAKFLVPDDVPTHFQEGIGKRGKDLRGAWMARFKD
                     YQTKYPELATHLYQMQHRQLPEGWDRKVPVFPPDAKGLAGREVSGIVLNALAATIPWL
                     LGGAADLAPSTKTRLTFEGAGDLTASNQSGRNIHFGVREHAMGAIVNGLSLSKVRPYG
                     SGFLIFSDYARPAIRLSALMEIPVVHIFTHDSIGVGEDGPTHQPIEQISSLRAIPGLM
                     VFRPGDANEIVEAWKVIMELRHEPVIMILSRQAIPTLDRMKYASAAGVAKGAYVLADP
                     LDGYPEVLLLGTGSEVPLCVQAHEQLAAEGIKSRVVSMPSWELFEQQSEDYRRSVIPS
                     DVLARVSVEQASVFGWANYVGTDGRSIGMKTFGASAPLKELAKKFGFTVEHVVAAAKE
                     QIARHQPHTISSQR"
     gene            14219..15367
                     /gene="tal"
                     /locus_tag="PKM72_05560"
     CDS             14219..15367
                     /gene="tal"
                     /locus_tag="PKM72_05560"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNP60285.1"
                     /translation="MNPLVQLREMGQSPWYDYIRRGLITSGELKALIENDGLMGMTSN
                     PSIFEKAIAGSADYDEAIAEVASQVTSVKQIYEGVAIQDIQDAADLMRPVYDASDGRD
                     GYVSLEVSPDLAFDTQGTIDEAVRLWKTVSRDNVMIKVPGTPEGLPAIEELLSQGINI
                     NVTLLFSVLMYEQVAWSYIRGLKRLAAHGGNLHRVASVASFFVSRIDSLIDKKLDEKI
                     AKESRPAQRAMMESLMGKVAIANAKLAYQIFQEVFASAEFQLLKVEGARVQRVLWAST
                     GTKNPKYPDTYYVDNLIGPDTVNTMPEATFMAFRDHGTVKNTIQEGLAEAKVTMQQLA
                     EAGISMEEVTDTLLKDGAKLFLDAFDQLMGVINGKRAKILAKNVDRVT"
     gene            15620..15772
                     /locus_tag="PKM72_05565"
     CDS             15620..15772
                     /locus_tag="PKM72_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60286.1"
                     /translation="MSEVWRMTFGVIAGYVELQVLDSAVPSPALWDLGAWLERLVAES
                     TRKERE"
     gene            complement(16708..18258)
                     /gene="zwf"
                     /locus_tag="PKM72_05570"
     CDS             complement(16708..18258)
                     /gene="zwf"
                     /locus_tag="PKM72_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNP60287.1"
                     /translation="MSPSDSSPKIDTPVTANRTASTQSCTIVIFGASGDLTQRKLLPA
                     LYNLLLDGLLPDNFAVLGLGRKPLSDDDFRSIARKGIEKFSRQTLEPDKWKSFQSRLF
                     YCAGDIVASDYYGTIRKRLKEIESPFKLPGNRIFYLAIPPTSFAPACEGLQNAGLVAA
                     PDQEDTYSRVIVEKPIGHDLSSAQAINTAIGNVFDESQIFRIDHYLGKETVQNIMVMR
                     FANSIFEPLWNHKYIDHVQLTVSEAVTLGTRATYYEGAGALRDMVQNHILQLLCLIAM
                     EPPYSLDPDVVRNARMDVLRGLRPIRGEDVEKMTVRAQYAHGNIKGQEVPGYRREEGV
                     QPDSTTETFVALKVFVENWRWAGVPFYIRTGKAMPTRASEIAVQFKDIPQILFNANPQ
                     IPQAPNLLTLRIQPEEGMALRIISKVPGSKARTHPVEMDFHYGDAFKAPSPEAYERLL
                     LDVMAGDTSLFMRRDAVEASWKWVTDILEGWSAYETKWLPEYPAGSWGPVEADRLIHA
                     GGHHWRKI"
     gene            complement(18280..19212)
                     /gene="gnd"
                     /locus_tag="PKM72_05575"
     CDS             complement(18280..19212)
                     /gene="gnd"
                     /locus_tag="PKM72_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNP60288.1"
                     /translation="MKRPPTVIIGESHMDIGFIGLGKMGMNMVTRLSQGGHRVVAYDR
                     SAALITEAETKGATAASSLEDLITKLPKPRVVWVMVPSGPPTEETVQYLGKILEVNDI
                     IIDGGNTKFHDDVRRAADLHPHGIHYIDAGTSGGIWGLQVGYCLMVGGKPEPVKRLAP
                     ILTTLAPENGWAHVGDHGAGHYVKMVHNGIEYSMMQGYAEGFELMSKSEYNLNLPAIA
                     DLWMHGSVVRSWLLELAAGALKQDPKLEQLQGYVQDSGEGRWMVMDAIEKDVPVPTLT
                     TALFTRFRSRQNTSFAEKMLAALRKAFGGHSVRL"
     gene            complement(19216..>20453)
                     /locus_tag="PKM72_05580"
     CDS             complement(19216..>20453)
                     /locus_tag="PKM72_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNP60289.1"
                     /translation="WQRWLAQCQYDGRWREVVHRSALALKLLTYAPTGAIVAAPTTSL
                     PENIGGPRNWDYRYTWIRDAAFSIYGLLRLGFTVEASRFMDWLNARCCELNPDGSIQL
                     MYGIDGRRDLTEEELPHLDGHRSSRPVRIGNGAASQLQMDMYGALMDAVYLYNKHGAS
                     ISYDLWVNLCRLLDFVCNNWEQPDEGIWEVRGGRRHFVYSKVMCWVALDRGIRLADKR
                     GFPGNRAQWMEERDRIYREIMERGWNAEIGAFVQSYDSEALDAANLIMPLVFFVSPTD
                     PRMLSTIDRTLEQLALGSLVYRYENGKAASDGLDSEEGTFSMCTFWLVEALTKAGRLT
                     EARLIFEKMLSYANHLRLYAEEVSHSGEQLGNFPQAFTHFALITAACNLDLALNTKRA
                     AHTVVRRHRPSSSPSRQSH"
BASE COUNT         4737 a         4714 c         5686 g         5316 t
ORIGIN      
        1 atccgatgcc catctttctg gttgaaaatg actgcccacc cttcagtctg gtgaaagatg
       61 gttgtctgag gacgacagag attacgtgta gtggtccatc tgttttttcg gtcgaatcct
      121 cgttgagttt aagggaaagc cggctgggaa gaatttttta taaagacgga tggggaggag
      181 agcgtcgatg cctgaaacat attctgatga acgcacattg ggtcgaagcc caatacgctg
      241 aaaagtggta cagcaaagag cgagaatttc ctccagtttg aataatcatg aagcaatcag
      301 gcgaataggg gtttccgatt cccctaggta gctggataaa gaaacgcccg tggcccgagg
      361 gaaaggatgt taggtagggg atcgcgagcg aaaaagaggc tgagcgaagc cagattttga
      421 cgatttcgtt ggcccgtagt gggactctgg tctaagaaaa cggcgaaata tggaccgctt
      481 aaacactttt gtagccgatt catcctaagt actacaggca cctaggcagg gaggctcgca
      541 agaaattgtt ggatcacggg catggcatca gggcgagcgg cgatgatgcc attgatggtg
      601 ggatgaggtt ggttgcattg aatgggtttc atatctctgt ccacgacgac ccctcctgcc
      661 gtttgaacca gaaggagggc agccgcgata tcccattcgt tttgtgtgcc aatattgatc
      721 actccgtcaa tctgtccggc ggccaccagt gccaatgaat aagcgatgga acccatcaga
      781 ctgcggcaat tggcggtttc tctccatgtg tgaatggtgg agcgggcaat gggtcctgtg
      841 ttcaccagaa atgagaagcg gtgtcctggg atctggggga catgaatggg gcagccattc
      901 acggttgccg gttcccctcg cacggcacaa aaatattccc gggtggccgg gttgaagata
      961 atcccgatgg aggcctgtcc atggtcgatg agcgcgagag agatggcgaa ttgtggcagg
     1021 cttttaataa agggtttggt cccatcgatg ggatccagga tccacacacg gttttttttc
     1081 agtctgaggg tgttgtcggg cgattcttcc gaaagccagc catcatcagg gtaggccgcg
     1141 aataaagctt cctgaatgat acgattgact tccagatcgg cattggtcac tggcgatccg
     1201 tccggcttga tctggacggc caagccttca tattggattc tgtgaagacg atcacttgca
     1261 aggagcatgg cttgtcgaat gatggaaagt tcttggcgat aatcagtcat gatgtcaatg
     1321 gttctggagt agtcgtcctg ggaagtaaat gctaattctt aatattatat cctggaccat
     1381 cggttaagtg tggagcgcac ccgaggcatc ggacctcaca gttcatagca tgagagacgc
     1441 gtccgaggct agattgtctg cttggtgatg tcggaatgaa agggttcggg atcatgagat
     1501 gagatctgag gagagttcct ggagcagtgt attatgcaaa ccgtgaccgt gagactgaaa
     1561 atttctcccg aacgctttaa ggcctactac catggtgcgg tggaatatgt ggtcgcaccg
     1621 tcaatcgatg gtcgaactat ccaatttcct gcaagggtcc ttcgtccttt tgtctcccat
     1681 caggggatcc aggggacatt tgaaatcacc ttcgatgcca atttaaagtt tcattcgatc
     1741 cgcttggcac aggatgacag gtgagcgcaa ccagtatgat tggggatgag tattcccttg
     1801 tcggattatt tctaagtgcg tttctttcct caacggtatt acctggaagc tccgaggttg
     1861 tgttcgtctt ttttgcatca cagggtcatt tccctgcctg gagtcttctc gccacggcca
     1921 cagctggtaa tacccttggt ggaatgagtt catgggcttt ggggtggctc atcgggtggt
     1981 ggtatccatt ccatgggttg acccaaccag ctcagcatct ggcagtggaa cgggttcgca
     2041 agtggggcag tccggtgctg ttattgtcct gggtgccgtt cattggggat ccgctctgca
     2101 tcgccgctgg gtggttgcgg gtgggctggg tccgggcact cgtgtgcatc gggatcggca
     2161 aagggtgccg gtatgcggtc ttgttcgccc tgttgcccac cagtggaggg tgaacggcga
     2221 aggcgtctga ctatagcatt cagaaaacgt tgttggtatt gttgcccttc cgtcttgcca
     2281 cgtaagtgcc aatatccttt ttttgtaaat ttctagtcca caggactatt tcttttacgt
     2341 ccaagcccaa tatggagccc tcatatcgtg aacgttcctc ttcttgattt aaaaacccaa
     2401 tacgccagca tcaaacaaga cattcaggtg gcgttggaca aggtgtgtgc ggagcaaagt
     2461 tttattctgg gcacccatgt tcaaaacctc gaacagaccc tggcgtcatt tatcggcacg
     2521 gatcatgcga ttggtgtggc gtccggaagc gatgcgttac tgttgtcgtt gatggaagtg
     2581 gggatagaac cgggcgatcg ggtcgtgacg gtccccttta cctttttcgc gtcggcagga
     2641 gtgatttccc ggcttcacgc acgtccggtc tttgtcgatg tgatgcctga tacgtttaat
     2701 ctcgatccca gccggttagc ggatagcttc acttccgagg tcaaagccat tctcccggtc
     2761 catctttttg gacaatgtgc ggacatggaa tccattgttc agattgcaga cgaaaaggga
     2821 atcccggtca ttgaggatgc ctgtcaggcc atcggtgccg ctcgaaacgg tgttcaggct
     2881 ggagcattcg gccgtacagg gtgcttcagc ttttttccgt caaaaaattt agggggattt
     2941 ggtgacggcg gggtgattac tacgcgggat cctctggttg cggaacgact gcaactcttg
     3001 cgtgtgcacg ggagccggtc agagtatcat catcatttca tcgggatgaa cagccggttg
     3061 gatgcgctgc aagcggccat cctccaggta aaatttcagc atttggctga gtggaccgcc
     3121 aagcgtcaag cacatgcagc gagttatctg aaattatttc agacatgcgg tcttgatgag
     3181 cgagtgaccg tgccggtggt gtcatccggc aatactcatg tttacaacca attcaccatc
     3241 cgcaccccac ggcgagacga actcagtacc tatctgaccc accatggaat aggtaatcgc
     3301 atttattacc ctgtccccct tcatcttcag gaatgctacc aggatctcgg gtatcataaa
     3361 ggcgattttc ctgtctcgga acaactctcc caagaggtgc tttcccttcc catttatcct
     3421 gaactcacag cggatcaact tcagtatgtg gttgacacga tcaaatcctt ctttgattgt
     3481 cgttaaagga gataacccca taattggggt ttaggactat tgagaacaag aaaatttatt
     3541 gagggtaccg ggcttgaatc acgctccgga gtccgcctct ggcggtaaac tccccggtga
     3601 cgatggctga cacagggcgg caggctttga cgaaatcggc cagaattttg ttgaccgcat
     3661 tttcataaaa gatgcccagg tttcgataag cgtggatata cattttgagg gattttaatt
     3721 caagacagga tttgtccggc acataggtca accggatggt tccaaagtca ggaaggcctg
     3781 ttttaggaca aatggccgta tattccggaa tctctatcgt aatttcatag cctggatatt
     3841 ggttaggcca ggtttcgatg cgtggtaatt tctcagcaat tccgctcttg gcatgtcgtt
     3901 ccgaatagcg taatcgtgaa gaagcgtggg gttttttcat gagtgaaagg ctcgtctcca
     3961 tggatgtttg aaaatggagc ctagtctact cttttttgaa atttcctcgc aacctcatgc
     4021 caccggtttc ttgagaatac ccctaccctg ggctatactc gctggacatt cctggcgcgc
     4081 ttggtatttt tctgaagccg tttttattca ccatccaaac gtacccttac cccgatcctg
     4141 agtctgacga gttaaaaagc gggggaggga attgtggagt ctgtctaaag cgatgagcag
     4201 tatcacgatg ttggaagctg acgattttgt gaaaggcctg caaggcattg gattcgattt
     4261 ttttaccggt gtgcccgaca cgattttggg gggcattatt gcccatttga cggaagaaca
     4321 gttgtatacc cctgcggttc gcgaagatga ggcggtggcg atggccgcag gtgcctacct
     4381 gggtgggaaa atcccggtcg tgttaatgca aaactccgga ttaggtaatg ctctgaatgt
     4441 tctggcttcg cttaatttga tttatcagat cccctgtttg ctattggtat cctggcgtgg
     4501 cttcgaaggc aaggacgctc ctgagcatct ggtgatgggg gaaacgatga cgacgctgtt
     4561 ggatacggtt cggattccac accggacgtt gtcccagaag acgattgtgg atgatctgaa
     4621 atggacggcc acgactttta tggagaagcg gatccctgtc gcactattgt tgaaaaaggg
     4681 aattgtgaaa accattcagc cctagtggga tgtcaaaaag cagtccgcat gaatctgaca
     4741 tgtgttctgg atcggtcacg tttggaaaga gtagtcaatc cgtaagtcta tacagtgagt
     4801 ggtcaacggg ttgattgtaa aagctaaaaa gtaaccattg tgttggagat gcaacgatga
     4861 ttggaccaga agaaggggtg atgcagagcc gggcgcaggc gatggcggct attttggaaa
     4921 tcatgaccga tcagctgctt gttgtctgca atgggtttcc ctcccgtgaa gcctgtaaac
     4981 tccgtgatcg ggctcaaaat ttttatatga ttggctccat gggcgcgacg gcagctatcg
     5041 gattaggatt ggctctggcc cagccagaaa aaacggtggt cgtatttgat ggcgacggaa
     5101 atgtgctgat gagtcttggc acgttggcca cgattagtgc gttgcgaccg aaaaatttca
     5161 ttcatgtggt ttttgataat gaagtgtatg ggaccaccgg caatcaaccc acctattcca
     5221 gggtggtggg cctggataaa atggccaaag ccgcaggata caagaatgtt gagcgcgtct
     5281 gggagcgaga agatattgta tatgagttta aggccatgtt aggagatgac gggccgaatt
     5341 ttttgttgat caaagtgaat gaacagcttg aagaggcgga tcgtattgcc ttgggtccag
     5401 ccgagctgac gaaacggttt aaggggagtg tcacctaacg acacaatgga tgtgttcgcg
     5461 tagaagccta atcgccagcc tgttaagcgt gtaaaggaag agggttcatg attttattca
     5521 atccaggtcc cgtcaatgtg tctgaaagag tgcgtcaggc gttacttcaa ccagatgtct
     5581 gtcatcgaga gtcggagttt tcggaattac tgtccgatat ccggcaaaaa ttgctgaagg
     5641 tctttgtgcc tggtaatgaa gacgagtaca ccgcgattgt cctgacgggt tccggcacgg
     5701 cggctgtgga agcggcgtta atgtccagta ttcctaccgg caagcgggcg atggtgatta
     5761 ataatggggt gtatggccaa cggatgtctt ccatgctggc gacgcaccgt ttgcaaacgc
     5821 cggacttaaa gtatgactgg ggagtcgcac cggatccgca ggcggttgat ttggcgctga
     5881 agcaacaccc ggaagtgcac accgtcgcga tggttcatca tgaaacgacg ctgggattga
     5941 ttaatcccgt aaaagaaatt gcagagatcg tggaccggta tggtcgggta tttatggtgg
     6001 actctgtgag cgggctgggg ggagaggcgc tggatattgc cggaagtaag ctgtacatgg
     6061 tcgcgggggc cgctcagaaa tgtatccaag gatttcctgg tgcagcattt gtgttggtcc
     6121 gaaaaggctt tatggagcgg gtaatgaaat atcccaagcg gtcctggtat ttgaacctgg
     6181 ctaattatta tgaggaacag gagcggggga cgattccctt tactccagcc gtgcaggtct
     6241 attatgcctt ccgggaagcc ctgaatgaac ttttagagga aggcctcgag aatcggattc
     6301 agcgatttca agggtatgcc accactattc ggacacgttt ggatgagtgg ggggtgaagg
     6361 cggtattgga tcctgccatt caaggtaata ccctaacggc gttttatctc ccggaagggt
     6421 gtacctacca aaggctgcat gatgagttaa agcaagctgg gtacgtgatc tatgccgggc
     6481 aagggcagtt ggaacagaaa atttttcggg tcgccaatat tggtgctctg accaatcagg
     6541 atattgaggg gtttctctcc gcctttcaga gcatagtttc gggggccagg gcatgaaggc
     6601 gattatcttc gcggctggcg tcggcaagcg cttgcagggt gtcacccagg gtcggccgaa
     6661 atgcctggtg gatattggcg ggaggacctt actgtcccgg catgtcgagt acctggggcg
     6721 attgggtgtg tctcaggtcg tattggtcgt ggggtatgct caagaccata ttcgaaaggc
     6781 catggctgcc gaatcatttg ctcaagagat ccgatgggtg gtgaatgaac agtttactcg
     6841 tggaagcatg acctcattgt gggcggcacg gtctgaaatg gacgatgatg tggtgctcat
     6901 ggatgccgat gttctctatg ccccatcgat tctgtctcga ttggttcatt cttccttccc
     6961 gacggccttg ttaatggatg agacagtgaa gcaggaatcg gaagaatgca tgattgccgc
     7021 gaagactgct cgggttctga ccctaagtaa aaccctgcca tccgactatg atgaggctgg
     7081 cgaaggggtc ggatttctca aagtccaaca acaggatatt ccagcattgt tgcaatcggt
     7141 tcgggcttat gtcgatgccg gagcgcttga tatggaatat gaggacacgc taaaggggtt
     7201 ttttgagaag gtgccggtgg gttacgaaaa aatcggaggg cttccttgga tagaaataga
     7261 ttttcccgaa gatatcgatc gagctgtgtc tgagatcttg cccgccgtga taaagttaga
     7321 ggagaatacc agggccacca gactgacgcc gtaggaatga gtgtatggac ggaaccctta
     7381 aacaagaagc cactcacgcg gttgacacgg caattttatt gacgtcggtc ggcgtcttta
     7441 attctggctg tgccgggacg ggtgacgcgg ttcccagtcc cttgactcgg gtcggcggca
     7501 tgacgctgtt tcaacgggcg gtgttcacgt tgcaacgagg agggatttct caaatatggg
     7561 tattggccgg gcctgaagaa caagcgcttc gtcaattact tcgggaagat aaccggatac
     7621 aggccgccgt tcgttggctt cctgtccgtg aatttcctcc tcatgaccct cagacttggg
     7681 aagcgcttgc ggaagagatc aatggagctt gtatgatcgt gggttgccat acagtgtact
     7741 ctccgacatt gatccaacgc ttgcgggctg aaggatctca aggaaaagcg gttgtggtcg
     7801 ttggccatcc tgaggagggg catcaccgag gaaatcctgg tgtggcgttc cggcctgaag
     7861 gaattgaagg acgatcaact tcaactgtgg tcttccatga ccaggcggta tcccaaacct
     7921 cacagtccaa aaccgacagt cagacttctg agcggagccg gttgccgctg gttggagatc
     7981 ttctggttct ccctgcccga ctcctgggta tttcaggcgt gttgcatgcc aatgggacca
     8041 atccactccg gttggcactg gagcaagcgg ctgtggaagg gacgattcaa accatcggcg
     8101 gagcttctca ttggttcagg gatgtccgtg gcccaaaggg accacaactc gcggagcgga
     8161 cgctgttgca ggcacttcag acactgaaag gcggcttgga tgggttggtg gatcgatatg
     8221 taaatcggaa atgttccggt ctgctcactc aggggttttt gcgtttggga tggtcaccga
     8281 acatgattac gatgctatcc atggtcgtgg gactcgtggc cgccgggctg tttgtgcctg
     8341 ggtcctggaa actcgccatt ttcggtggac tgattttgca gttatctgtg attattgatt
     8401 gttgtgatgg agaagtggcc aggctgacgt tttctgagtc aaagtttggg caggaactcg
     8461 atatttgggc agataatgtt gtgcatatcg tgttgtttgc cgccattgcc tgcggggcgt
     8521 ttctccatgg gccgtgggaa catacgcacc ttcctcttct gcttggggcc tcagccgtct
     8581 tagccaatgt cgtgtcgttg ctgttagtca atcatgccag gcacttgcga tcccgtcctc
     8641 gtgagcctcg gcaattcact gaacaggagc aagctaatat cgaatttatg ttaggcaatg
     8701 tcgccaatcg cgatttttcg atcatcgtct tgttgggtgc tggttttggc ctcctgcact
     8761 ggtttttggc cctggccgca attggctcgt ggctctttgt catgtcgatg gcctggatgc
     8821 ttcgccgtag tctcatctct cgtgcttaaa ctgcttttcc tggtcgttgg cctcacggct
     8881 ttggtcggaa ttattctcca catcggcctc gaaccgattc agcacaccgt ttctcactta
     8941 ggtctcctac atctcctact catcctcatt cccatggttt tcgtatatgg gttggaagct
     9001 ttgggatggc aattcacgct gggtttccat gctcgaaagg tcgggttcat gcgattattt
     9061 gccattagga tggctggcga aaccgtgaat gtcacgactc ccacggccta tgtcggtggc
     9121 gaacctctga aggcctacct cctgaaacgt tacggggtgc cgatggtgga gggattggcg
     9181 tccgtgatta ccgcgaagac cacgatgacc ttcgcacaag tgctttttat cttgttgggg
     9241 ttggccttgg ctttttggat acttggcgat tcaggtcatt actgggtggc gatgttggtc
     9301 agtgtcggag tgttagcgtt tggcgtcggg ctgtttgtgc tgctccaacg ccacggtctc
     9361 ggtatggggt gcttgggcct attgcgagca tgcggtatca ggttcacctt tcttgaaaaa
     9421 cgagaagctc aactccagga gctggacggc acgattcgga aattttattc tcagcaccgg
     9481 gagacctttt atgcggcgtt ggctgtgttt tttctggcat ggatgatgga aacgcttgag
     9541 gtgtatgcca tactctactt tctcgatgtg agcatcgatg tgtgggtagc catatcaatt
     9601 gcggcgctca ccgtgctcat caagggcgga acatttttta ttcccggaag cctgggtgcg
     9661 caagaagggg gatacacctt actattgatg acttttggct actcggaagt gacgggaatc
     9721 acctttgccc ttattcgacg aatgcgagaa attctctgga ttggctttgg tcttgtgtgc
     9781 ctgatgctcc tgaaaggcca tgacggtgga tcggatcaga tacactcgat ggaacggtcg
     9841 ggggacaaat aggctaagtg aaaatatccg gtgacttgaa ctcttcgggt ctattgtgaa
     9901 taatcggacc ctcacggatt ttcagacgag acgagaaagg atttgggaac gatggatatt
     9961 gaactgggaa cgaataccct caggaatacc gatggtgtct ttatcgctca tggcaaagaa
    10021 cagcttcgta ttgagtggtt ggaagaggag aaaaaactgg cattgagtat gggggtgttt
    10081 atgcccacgg gaacggaagt ggccaagttg caacgcaatg cctgggaaca taattccggc
    10141 gatcggtttg tcctcactga gttgccggat agagtgaagg tcgaagatac aaccctgaaa
    10201 actctcgtct tggaaataca caaaaaatct cacgaagccg tggctatccc ttcggcaaaa
    10261 ttctatacct cgaaaggcac cttgtctgaa atctcccttg aatggtggcg ggttggtaat
    10321 aaaatggagc tggccggcat tgatatggac ctcgaaggcg gcagtgtgga attccccgaa
    10381 taactcctcc tcggccattc tccgtttttt taaaggtatc gggttatgtc caatatgttg
    10441 tgatgctctc tggcctcttt cttggagttt accagtgatg gatgactccc cttcatggcc
    10501 cacatgttga agggccaatt cccctattgc ttccagttaa ttaataatgt ggaaaccgtc
    10561 gctatgaatt tggatcccag atcaatggcg cacatatttc tttgtgattg ggcaaaggtt
    10621 ttagcaagag tcaggcaggc gggtttctgc ggggtcgtga ctattctgtc ttcgattttc
    10681 taaaggccat tgataatggc catgcaaaaa aagtgaacct acttcactgc cgttttggcg
    10741 cgggccatca tgcggcaaaa ggagcaggtt cccgcagttg tgggttggct gcacacggta
    10801 cagggttgca acgtggtttg gtcaatttcg ctcatggatg gggcgacgga agaggatttt
    10861 tcctgtctat caaggaaccc ccagtaaaat cgttgcttgg ttcccggaga ttctgcttct
    10921 aagcgattca acgcgtcttt atacactaac atcttcgcac ctttggccat cgggcattct
    10981 tcaaccaaat agtcaatccg gttgaccacg gcataggcgg caatttcccg ttcggtcatg
    11041 cggtagagcg gtttcacttt tttggcaaaa ccttcgacgg atgcgggaag gcttggcgat
    11101 tgtttctgca aatattcctc ctgccactgc agaacattcc ccagaagtct ggcagcctca
    11161 tcatctaaat tatggcctgt ggccatcacg ttgtaatggt tttgccaggc cacccgattg
    11221 aattgatacc gtttaattgt gccacaggct gagcaggtcg ggcgtttgat catattggct
    11281 aattctttaa ttcccgctcc tgcatcttct tccacggtat gaatggtgag ttgggacccg
    11341 cagggttcag ccaccacttt tgcaaactgt tcgacttttt cttttgatcg gctggaataa
    11401 ccgggaatgc ctaaatccac gtatagtgca tcgacccggt agcctaattt cagcagaatt
    11461 tcccataggg tcagactgtc cttccctccg gatacggcaa ccagaatgcg atcttcaggg
    11521 gaaaacatcc gttgagcctt aatggctcgt tgcacctggg ttcgcacaaa ttcagtgagg
    11581 cagtccccac aaaacgccgt attgtgcctt ggcagtccca gtaccgcccg ttttggacat
    11641 tttcggcagc gcatgcgttt accctccgga tatgaccggg cggatttcaa tgctatcccc
    11701 atccgccacg gtttcgtctt cggtcatgag gtcctgccct cggatgatca ggaaggcctc
    11761 ggggattaaa ttgagttctt tgaaaatgtc ggcaacccgt ttgggtccct ggagcaccac
    11821 gtcacgggtg ggatggctga gatggacttt catgggttcg aatcataaag acaggttcat
    11881 gggattggca acttattaaa ttagactact ttttatctta accagaggta aactttttgt
    11941 tactgctatt gagtcggccg ggccgtttgc tttaatacct attgggtaag aatgtagagt
    12001 ggaaggatga gctatgatag gatgcagata ctcacctttc tcacaaaata aaccctcgtc
    12061 acgcgagata gctcacttat tatggataac gaaggagcaa gaatgaaacc cgcatgtgat
    12121 tcgctggacc agctttgtat taacaccatt cgtaccttat cgatggatgc ggtgcaggcc
    12181 gcgaactccg ggcatcccgg taccccaatg gctctggcgc cggtcgccta ctggctctgg
    12241 aatcggatcc tgcgttctga tcccgacgat cccatctggc cgaatcggga ccggtttatt
    12301 ctctcggccg ggcatgcgtc gatgttgctc tattccttgt tgcatctctg tggggtgaag
    12361 tcggtgaacg gacaatatga acacctcggc gagccctcag tcacattgga ggatatcaaa
    12421 cggtttcggc aattagagag taaatgtcct ggtcatcctg aatatcgttg gacatctggt
    12481 atcgagacca caaccggacc tttagggcaa ggggtggcga caagtgtcgg tatggcgatt
    12541 gcccaacaat ggatggcggg ctattttaat cggccagatt ttgaaatgtt taattacaat
    12601 gtgtattcca tctgtggtga tggatgtctc atggagggag tgtcatcgga agcggcgtct
    12661 cttgccggac atcttaagtt gtcaaatctc tgttggatct acgacaacaa taagatcacc
    12721 attgaaggcc ctacggactt ggcctttagc gaagatgtgg ctaccaggtt tatcgcctat
    12781 gggtggaatg tgacccgtgt gggtgatgcc aatgatctta atatgctgga tcgcgccttt
    12841 ggcacattct tgaaggaatt agaacgtcca acgttgatca ttgtggatag ccatattggg
    12901 tatggagctc cgaataaaca ggatacgcat tccgctcatg gtgaaccgtt gggtgaagaa
    12961 gaaattcgtt tggccaagcg tcattatggt tggccggaag atgccaaatt tcttgtgccc
    13021 gatgatgtgc cgactcattt tcaagagggg attgggaagc gaggcaaaga tttgcgtggt
    13081 gcctggatgg cccgttttaa agactatcag accaaatatc cggaattggc tactcacttg
    13141 tatcagatgc agcatagaca attgccagag ggatgggacc gtaaagttcc tgttttcccg
    13201 cctgatgcca aggggcttgc cggtcgggaa gtgtctggaa ttgtattaaa tgcgttggca
    13261 gcgacgattc cttggcttct tggcggggcc gcagatttgg ctccgtccac aaagactcga
    13321 ttaacctttg agggagccgg ggatttgacc gccagcaatc aatcaggacg aaatatccat
    13381 ttcggcgttc gagagcatgc gatgggtgct atcgtgaatg gcctgtcttt gtcaaaagtc
    13441 cgcccttatg gctcaggatt tctcatcttt agcgattatg ccagaccggc tatccggtta
    13501 agtgccttaa tggaaattcc cgttgtgcat atttttaccc atgattctat tggggtagga
    13561 gaagatgggc cgacacacca gcccattgaa caaatctctt cgcttcgggc gattccaggg
    13621 ttaatggtat ttcgccctgg cgatgccaac gaaattgtcg aagcgtggaa ggtaattatg
    13681 gaattgcggc atgaaccggt gattatgatt ctctcccggc aggcgattcc cacgttggat
    13741 cgcatgaaat atgcgtctgc agccggcgta gccaaaggcg cctatgtatt ggcggaccca
    13801 ttggacggct atcccgaggt attattgctt ggaacaggaa gcgaagtgcc attgtgtgtg
    13861 caagcccatg agcaattggc cgctgaaggg attaaaagtc gggtcgtcag tatgccttct
    13921 tgggagttat tcgaacaaca atcggaagac tatcgtcgct cggttattcc ctccgatgtc
    13981 ctcgcgcggg tatcggttga acaagcgtcc gtctttggat gggcgaatta tgtcgggacc
    14041 gatggtcggt caattggcat gaaaacattt ggcgcctcgg ctccactcaa ggagttagcc
    14101 aaaaaatttg ggtttacggt tgagcatgta gtggccgcag cgaaggagca gatcgcacgg
    14161 caccaacctc acaccatcag ctctcaacgc taaccatttt tttaaaggaa acatcaccat
    14221 gaatccactc gttcaacttc gtgaaatggg ccaaagtccc tggtatgact atattcgtcg
    14281 gggtttgatt acctctggag aactcaaggc attaattgag aacgatggtc tcatgggcat
    14341 gacctcgaat ccctcgattt ttgaaaaagc gattgccggg agtgcggatt atgatgaggc
    14401 gattgcagag gtcgcgtctc aggttacgag cgtcaagcag atatatgaag gagtggccat
    14461 tcaagacatc caggatgccg ccgacttgat gcggccggtc tatgatgcct cggatggtcg
    14521 tgatggctat gtgagcttgg aggtctctcc ggacttggct tttgatacgc aaggcaccat
    14581 cgatgaagcc gtccgtttgt ggaaaaccgt gagccgggat aatgtcatga ttaaagtacc
    14641 aggaactccg gaagggctac ccgccatcga ggagcttctg tcccagggca tcaatatcaa
    14701 tgtgaccctg ttattcagtg tgttgatgta tgagcaggtg gcttggagtt atatccgtgg
    14761 gttgaaacgg ttggccgcgc atggggggaa tttacatcgc gtcgcgtcgg tggccagctt
    14821 tttcgtcagc cggattgata gtttgattga taaaaaattg gacgagaaaa tcgcaaaaga
    14881 atctcgtccc gcccaacgag cgatgatgga aagtcttatg ggaaaggttg ctattgccaa
    14941 tgccaagttg gcctatcaaa tctttcagga agtctttgcg agtgctgaat ttcaacttct
    15001 caaagtagaa ggtgcccgcg ttcagcgggt cctttgggcc agtaccggca cgaaaaatcc
    15061 caaatatccg gatacctatt atgtcgataa tcttatcggt ccggatacgg tgaatacgat
    15121 gcctgaagcg acatttatgg ctttccggga ccatggaaca gtgaagaata cgatccagga
    15181 aggcctggct gaagccaaag tcaccatgca gcagttggcc gaggccggaa tttcgatgga
    15241 ggaggtgacc gatactctcc tgaaagatgg cgcaaaattg tttctggatg cctttgatca
    15301 attaatgggc gtgattaacg ggaagcgcgc gaaaatatta gcgaagaatg tggatcgggt
    15361 cacctaattc aaccggccct ctgtagccga ttgacgaggc aataaaagaa ccacaatctg
    15421 gtcagccgtt cgtggagcgt tgatccaacc gtttggtatc acggttccgc tcatcatgag
    15481 aatattcgga atgcgccggg acggttgcgg atcacggaac tctcattttt taatataccc
    15541 ccattgctgt ctttggcaaa agaaatcaag gaagcgggtt tcaaacatgt tcgggtcctg
    15601 aacatggggg aagcagcttg tgtcggaagt ctggcggatg acctttggcg tcattgccgg
    15661 atatgtcgaa ttacaagtgt tggatagcgc ggtgccctcg ccggcactat gggatctggg
    15721 tgcgtggtta gaacggttag tggctgaaag cacgaggaaa gaaagggaat aattccgatg
    15781 atgggaaatg cgtaggggca cctgaggtct atggcaagac cgggtctttg cctacatccc
    15841 atataaacaa gagattgatg ccacacaggg ggccaaggtg aaggctcttg aacaagccgg
    15901 gtatccggtg attcgcatca acgtcgctgc cctggttaat cttggtaagc cattttttta
    15961 atggtaaatg gcgccggcgg cggtttgaag gctgttagag ataaacgcgt ttggtcagcc
    16021 aaacgtccag gaaagtaaga attacacgaa gaaatatttt gaagccttta cccggacggc
    16081 actttgcccg actccctacc ttactcattg atggcaatgt cacagtcttg accgataaaa
    16141 aaatgcttca gtcctttaaa ggtaatgctt cattggaaac cgtgctgagg gggcatctcg
    16201 ctggtattaa aacaggtgat tatttcgcca tcaatgctac gtggagccta tcgatgccgt
    16261 tcgtgcaatt tttcaactca tgcgcaccaa cattcatgag atcaaacagg acgcgaccaa
    16321 atgggtatgc cccacggatt tttgcattca accggacaat tgcattaagg cggccccgat
    16381 tctggtgtgt cttttcaggt gatatgtgat gaccgggagg acttcccatt cccgatgaac
    16441 catattcgtt tgaggtgctg aagccgggca agtattgagt gataggcaag gcctaagcag
    16501 tcgtcaacgt tgggtctatc gtctccagct aggggccgat gttgaaaagg ggttggttcg
    16561 tctggagcga ttgatggaaa ccaacatcgg tctgcatgct caccaggcat aattaaggat
    16621 gaggatgttg agcagttttg gcgagcagtg aagcgagggt aaccctctaa agtattctca
    16681 atggggttac ttgagcgacg ggctggacta aatttttcgc caatggtggc cgcctgcatg
    16741 aatgagtcga tcggcctcaa ctggtcccca actcccggct ggatactctg gcaaccactt
    16801 ggtttcgtat gcactccaac cttcaaggat atctgtaacc catttccacg aggcctcaac
    16861 ggcatcccgc ctcatgaata aagatgtatc cccggccatg acatccaaca acaatcgctc
    16921 gtaggcttcc ggggacggtg ccttaaaggc atcgccgtag tgaaagtcca tctccacggg
    16981 atgagttctg gctttggagc caggcacttt tgaaataatg cgaagagcca tgccctcttc
    17041 gggttgaatg cgaagcgtga gcaaattggg agcctgggga atttgtggat tggcattaaa
    17101 tagaatctgg ggaatgtcct tgaattggac ggcgatttcg ctggctcgtg tcggcatggc
    17161 ttttccggtg cgaatataga agggcactcc ggcccagcgc cagttttcca caaagacttt
    17221 taaggcaacg aatgtttccg tggtggagtc aggttgaact ccttcctctc gtcgatagcc
    17281 tggtacctcc tggcccttga tgttgccgtg ggcatactga gcgcgaacgg tcatcttttc
    17341 aacgtcttct ccacgaattg ggcgcagtcc gcgtaacaca tccattcgag cgttccgaac
    17401 cacatcgggg tccagcgagt aggggggctc cattgcaatc agacagagca gttgcaaaat
    17461 gtgattttgc accatgtctc gaagggctcc tgccccctcg taataggtgg ctcgcgtgcc
    17521 aagggtaacg gcttcgctga cggtcaattg cacgtggtcg atatatttat ggttccatag
    17581 cggttcaaaa atggagttgg cgaaccgcat aaccataata ttctggacag tttccttccc
    17641 caaataatga tcgatgcgaa aaatttgtga ttcatcgaag acatttccaa tagccgtatt
    17701 aatggcttga gcggaagaca aatcgtgtcc aattggtttt tccacaatga ctcgggagta
    17761 cgtgtcctct tgatcaggcg cagccactaa cccggcgttc tggagcccct cacatgcggg
    17821 cgcaaaagaa gttggcggga tggctaaata aaagatgcga ttgcctggta gtttgaacgg
    17881 tgattcaatc tcctttagtc gtttccggat tgttccatag tagtcggagg cgacaatatc
    17941 ccctgcacaa taaaacaagc gactttggaa agatttccac ttgtcgggtt caagcgtttg
    18001 ccgggaaaac ttttcaatgc ctttacgggc gatactgcga aaatcatcat ccgacagagg
    18061 tttccgtcct aaccccagta cggcaaaatt gtccggtagc aagccgtcca ggagaaggtt
    18121 atagagggca ggcagaagtt tgcgttgggt cagatcgcct gaggcaccaa agatgactat
    18181 cgtgcatgat tgagtcgatg cagttcggtt agccgtgacg ggtgtgtcga ttttaggaga
    18241 agaatcactc ggtgacatgt tgacctcgca agaaacgggt tatagtcgga cgctatgtcc
    18301 cccaaaagcc tttcgcagtg ctgctaacat tttttccgcg aaggaggtgt tctgccggga
    18361 gcggaatcgc gtgaataacg cggtcgtcag agtgggaacc ggtacgtcct tttcaatggc
    18421 atccataacc atccatcgtc cctcgccgga atcctgaacg tagccttgta attgttccag
    18481 cttaggatcc tgtttaagtg ctccggctgc tagttcaagt agccaggacc tgactacact
    18541 gccatgcatc catagatcgg cgattgcggg gagattcaag ttatattcac ttttggacat
    18601 gagctcgaag ccttccgcat atccctgcat catactatat tcaatgccat tgtgaaccat
    18661 cttgacgtaa tgtccagcgc catggtcccc cacatgggcc catccatttt ccggagccaa
    18721 agttgtgagg atgggggcca atcgtttgac gggttccggt tttccaccga ccatcaaaca
    18781 atagccgact tgcaatcccc aaataccacc gctggttccg gcatcaatat aatggatgcc
    18841 atgggggtga agatcggcag cccggcgaac gtcatcatga aattttgtat tgcctccatc
    18901 gatgatgata tcgttcacct ccaggatctt tccaagatat tgtacggtct cttcagtggg
    18961 tggccctgag ggtaccatga cccataccac tcgcggtttg ggtaatttgg tgatcaaatc
    19021 ttccaatgac gaagccgcag ttgccccttt tgtttcggcc tcggtgatta gggccgccga
    19081 gcggtcatag gccacgaccc gatgtcctcc ctggctcaat cgagtgacca tgttcatccc
    19141 cattttgccg agtccaataa aaccgatgtc catatgcgat tctcctatga tcacggttgg
    19201 tgggcgcttc atttctcaat gggactgacg tgagggcgat gatgacggtc gatgacgcct
    19261 cacgaccgtg tgggcagccc gtttcgtatt gagagccaga tcaagattgc aggcggccgt
    19321 aatgagagca aaatgcgtaa aggcctgggg gaaatttcct aactgttcac ccgagtgtga
    19381 gacttcctcg gcatataatc gaagatgatt tgcatagctt aacatttttt caaagatcag
    19441 tcgcgcttcc gtcaatcgcc cagctttcgt cagggcttcc acaagccaaa aggtacacat
    19501 gctaaaagtg ccttcttcgc tgtccaggcc atccgatgcg gccttcccgt tttcataacg
    19561 gtacacgagg cttcctaatg ccagttgctc gagtgtgcgg tcaatggttg agagcatgcg
    19621 aggatcggtg ggagacacga agaacacgag aggcatgatc aggttggcgg catccaacgc
    19681 ttcgctgtcg taggattgga caaaggcccc aatctcggca ttccaacccc gttccataat
    19741 ttcccggtag atccggtcgc gttcctccat ccattgagcc ctgttacctg gaaatcctcg
    19801 tttatcggct aaacgaatgc cgcggtctaa ggctacccag cacattactt tggaatagac
    19861 aaaatgacgg cgtcctcctc tgacttccca aatgccttca tcaggttgct cccaattgtt
    19921 gcagacgaaa tccaggagac gacagagatt cacccagaga tcgtaggaaa tggacgcgcc
    19981 gtgtttattg taaaggtaga cggcgtccat cagtgcgcca tacatatcca tttgcagttg
    20041 agaagcggcg ccattgccga tgcgaacagg gcgggaactg cgatggccat ccaggtgggg
    20101 caattcttct tcggtcagat cacgacgacc gtcgatgcca tacatcaatt ggatagatcc
    20161 gtccggattg agttcgcaac aacgggcatt cagccaatcc atgaaccgac tggcttccac
    20221 cgtaaatccc agacgtaata gcccatatat agaaaaggcc gcatcccgaa tccaggtata
    20281 gcgatagtcc caatttcgag ggcccccgat attttcagga agactggttg tgggagccgc
    20341 tacaatggct ccggtgggag cataggtcag gagtttgagg gctaaggcgg atcgatggac
    20401 cacttcccgc catcgcccat cgtattggca ttgtgccagc cagcgctgcc aga
//
LOCUS       DAORAQ010000038        20120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_486_length_20120_cov_69.381610, whole genome shotgun sequence.
ACCESSION   DAORAQ010000038 DAORAQ010000000
VERSION     DAORAQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 20120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20120
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_486_length_20120_cov_69.381610"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..503)
                     /locus_tag="PKM72_05585"
     CDS             complement(120..503)
                     /locus_tag="PKM72_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP60290.1"
                     /translation="MRLIKDGGMKQSFYGGTLAFVMIMCLSVVEPGYPGTILNGDQPD
                     ALPPSEERLQQGKALYEGVARCMHCHGRTAVRRPLTKQELFSIIQLGVPGTSHMPFKH
                     LLSDEEIWSIVYYQLHDICKHGCTD"
     gene            1139..1306
                     /locus_tag="PKM72_05590"
     CDS             1139..1306
                     /locus_tag="PKM72_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60291.1"
                     /translation="MTEIIGGSAAYQYVWSGMIMAGSLWGLFLSSRMIPLFTRSLTRH
                     SRNISGVKDVP"
     gene            1398..1895
                     /locus_tag="PKM72_05595"
     CDS             1398..1895
                     /locus_tag="PKM72_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNP60292.1"
                     /translation="MMNMIRPTIQHRIHQLAGMIAHVGQAAIVSMILAFSLGTPYLLQ
                     AEPASQAMTKEQAIKLGEDFGIIVGEVDEEIRDYLGLERAKGVVVFEVIGGKPAALAG
                     IKPRALIKEINSVEVTTLLDFGLALQDALPTENFSVATYEPSDPDNQGISGGINFHFV
                     RVEKS"
     gene            complement(2310..2747)
                     /locus_tag="PKM72_05600"
     CDS             complement(2310..2747)
                     /locus_tag="PKM72_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNP60293.1"
                     /translation="MKLPPSSFGCPLDSLLRILAGPWTIYILCRLHTNGETRFGQLKR
                     QMPGISSKMLTERLRSLEKAEIIFRRQVSTIPLQVTYGLTTEGRELTTILDQINTLAD
                     RWQDQETKTANSSVQVKEAKRRKAVSNDSVMPPLKKGRTTRKN"
     gene            2846..3082
                     /locus_tag="PKM72_05605"
     CDS             2846..3082
                     /locus_tag="PKM72_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60294.1"
                     /translation="MFMTTDQMSAERTMKCHRCAGLLVRDSIYNLDGQFHHLEVLRCL
                     NCGDTVDPTILKARGMKNGQRGRMELQFSSGHVH"
     gene            complement(3377..3988)
                     /locus_tag="PKM72_05610"
     CDS             complement(3377..3988)
                     /locus_tag="PKM72_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60295.1"
                     /translation="MIVNFSRFIILSFIFTTGFIGGCSESSTDKSTPEPRSEHSAFIA
                     NVDGAVHADIWGDGIVTYLPPKEGTVSGNRPGYYMLVNNLPRDDMKGREFFIIFRIPD
                     GAQPGHYNLMAPDPLKVGQNFDVQIEAVEKGQSIAYQNNTEGTMTLDSFSPDRLSPEA
                     STIKGTFQFVTENNAGERVSVEGTFDFPSEKNVFSLTAAWVMV"
     gene            complement(4081..4464)
                     /locus_tag="PKM72_05615"
     CDS             complement(4081..4464)
                     /locus_tag="PKM72_05615"
                     /inference="COORDINATES: protein motif:HMM:NF025140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HNP60296.1"
                     /translation="MKSQQSNLKNFGIVACFAFVCLLMPAAVLPAADGSSQSAPEEMV
                     RKNLEPFAGAYKEVSELSEIYAQRILQSEPGQSDALQEEANQKMTQAVAKHGLSVEEY
                     NLIFQTIQSEPGLQDEFMIALQRGH"
     gene            complement(4557..4988)
                     /locus_tag="PKM72_05620"
     CDS             complement(4557..4988)
                     /locus_tag="PKM72_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP60297.1"
                     /translation="MTTIAGMSATGYSTVGDIVPTHMTIYLAQSEGNSAIMDILSSHL
                     VVAPVVDDEGHLAGFLGESEILNALYSGKDISQVKVEDIMTEDRTSTVTEMTPISAVV
                     TLFQDEELQIIPITREGRVIGSLTRHDLIRALTGAGLGIEN"
     gene            5858..6937
                     /locus_tag="PKM72_05625"
     CDS             5858..6937
                     /locus_tag="PKM72_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP60298.1"
                     /translation="MRIAQLAPLAESVPPKQYGGTERIVSYLTEELVRQGHDVTLFAS
                     GDSQTNAHLKSMCREGLRKTNGVSNPQAPFTMMLEKTFSSADQFDLFHSHLDFLAFPL
                     ARRCGTPVLTTLHGRLDLPELKPIFAEFLDCPLISISNAQRNPLTWANWKQTIYHGLP
                     ENLYSFHPESGSYLVFVGRLTPEKRPDHAIEIAKRVGMPLRIAAKVDPVDQEYFHTVI
                     KPLLSDPLVEYVGEVTDAEKNDLIGNAFALVAPFDWPEPFGLVFIEALACGTPVLAYR
                     RGSVPEIIDDGHTGFICDSIEHMIKAVPCIASLDRQPCRLAFEQRFTVKRMVQDYLRA
                     YENLLNVQEENYHPSLNGRETAVNF"
     gene            6993..8522
                     /locus_tag="PKM72_05630"
     CDS             6993..8522
                     /locus_tag="PKM72_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP60299.1"
                     /translation="MATSNHLDTLPNRWDLFRNRNFRMLLASQAVSQIGDGLTKVALL
                     WFVYQLTGSALKMTIIGLLQTIPPLVLSPVIGVYLDRLPKKPVMVILDVIRAFLIALI
                     PVLYVFDALTLEVLFILVFFTAIFSTAFGPALAASLPLLVKPSELMSANSLLQSTTNM
                     GILIGPAICGVLIAWLGTQNVLYVDAATFLLSALCLLPIKGHLLTTAPRIANATSNIS
                     QDLAVGMRFVFREKPVLRALLITTVFYTLGASAFIYVLPLFVNDHIEADPRWLGWLWS
                     AMGAGMLATSFGLGCVKKLERKSRTFLMAFSMVVGGSALQFLAHSQSVMWGFILVTLI
                     GASTAAFTPIAWSIIQETTPNHLMGRVFTLINTASMAMASGGMVAFGGVADNFGSMAG
                     ISMIGFVFLITGMVVIFSNRSPIQAEEPLPWKAMESPDHSDFKRAWHPVRMGNSTSPL
                     DRPWTRNLQWISIKSPYPFRQIPSVRPASGSRAPPDQQKFRMSPQTLIYKNEVYEAIP
                     P"
     gene            8753..9436
                     /locus_tag="PKM72_05635"
     CDS             8753..9436
                     /locus_tag="PKM72_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNP60300.1"
                     /translation="MTNFNMQIINKILMKSTFTISCIIWGHIWAINVTHGYDVPISVP
                     GGTHILRVQNHQTNPSPKKEFSGEDQRKTSANIIESRNLELKLAFMGDPRLFPFDIDC
                     QIQEKTVKLTGSVTLEEEKTLATLLASHLIREKEIVNLIEVRPSLSATLQAGMDTRLT
                     ELVKQRFAQSQTLRDANFEIITIRGIVSLKGQTRFQVIALEAAQAAREIPGVIAVLTQ
                     NIRLEAGNE"
     gene            9457..10746
                     /locus_tag="PKM72_05640"
     CDS             9457..10746
                     /locus_tag="PKM72_05640"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP60301.1"
                     /translation="MAKLSLSSPTLLQSVSIATNPPLLLQRNFGIIWWSQLISQIGDG
                     VTKLALLWFVYAVTGSALQTTIIGILQTLPPIVLGPFIGVFIDRFPKKIILIGTDLIR
                     ALVIGVIPCLVSHEAFTVEFLYVLVFCNAVATSLFAPALFASIPFIVAKSALTPANAL
                     LQSTMSLGVIFGPLLSGLGIALLSSQEVLCINVLTYIGSALLLVFVSIPEVTHRAKTF
                     QSSLTNYVKDLQEGVQFTFFQQPVILPLMLTAGLYSFVMSAFHTLLPVFGRNMLKFGP
                     VEVGYVTSGVGVGLFLASLFLAKVNLGAQWRRIRLLATTSLMAAFAIWALTGAIGLPL
                     SLAIVVVIGMGSGVLTPIEWGIIQEISPPQLIGRVLALYGTLAMLSAIGGMTMFGWIM
                     EQFGEIWTLLGMGAVLLITSQVAFKLSRRVRSHSNYD"
     gene            complement(11095..11541)
                     /locus_tag="PKM72_05645"
     CDS             complement(11095..11541)
                     /locus_tag="PKM72_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60302.1"
                     /translation="MTTQLLGNIFNGYDFVTIREPLPRKGSTGKTVMVVDDEKDFRLL
                     LRDRLVRLGLICVEAGNGEEAKVQLKKNHVDMVVTDFRMPKMDGLELIDWLQQYHRDL
                     PIIFVSGDISVPVRQKAEQAGVYAILEKPCSLSDLSLKIVEVFDEL"
     gene            complement(11814..12437)
                     /locus_tag="PKM72_05650"
     CDS             complement(11814..12437)
                     /locus_tag="PKM72_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60303.1"
                     /translation="MKRQNFNHFHIAIGVSVIVMVGVLGLNPSTDMAMFSDAPRKDVI
                     QGQGDIGQITIEGEVTRIQGEFVGKDFSQMKDRRYKVETPLGKVWDLHLGEKTQKIGE
                     IFLGDRVKAIIERNGTIQTVQKIEQNNAYSNNSVLPRQISGRVERVDGNFLVVNQGEN
                     NEILHLDERSTLEGNIYEGSTIIAQLGNAGYAIKIQEFQDEPGISSY"
     gene            complement(12531..12854)
                     /locus_tag="PKM72_05655"
     CDS             complement(12531..12854)
                     /locus_tag="PKM72_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60304.1"
                     /translation="MKIFSYFVIGFFISIWVSPIAEASEVRGTTNDLTEQAHQKELLT
                     MVSGTVQDVQGDWCVVEDFEGTEWKIQVDKYTDTIGRVLPGVTIIAMVEGDGHAKKVK
                     VLMNE"
     gene            complement(13228..13599)
                     /locus_tag="PKM72_05660"
     CDS             complement(13228..13599)
                     /locus_tag="PKM72_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60305.1"
                     /translation="MTKKNVLIVEDDLNSREGLQSMLRMYGYHADTSQDGLQAIKKIK
                     ENFFEVAVIDINLPPVMNVEINGWDLIRIFRSFNPSIDIIVVSGEKGIQARAQQFNLA
                     GCLEKPIRPAHLKSLMETLGG"
     gene            complement(13716..14072)
                     /locus_tag="PKM72_05665"
     CDS             complement(13716..14072)
                     /locus_tag="PKM72_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60306.1"
                     /translation="MSSEPIPGKNEIPSDKPQQHVPDSQEQKPAILVPEIPNKDNRQE
                     IVTYKSKIDETQNLLRTINESQLSKEQHDTYISINSFLEKAQEAFSQNDLSMALNLSE
                     KAHTLTKEIVNNSTKP"
     gene            complement(14305..16119)
                     /locus_tag="PKM72_05670"
     CDS             complement(14305..16119)
                     /locus_tag="PKM72_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60307.1"
                     /translation="MMRLNVKGKEALGVTVLTLVVVVGSTFLNLSQLSRVIVQETSEQ
                     VSLIKRQIFEYSKRVVLNAQSPDTHPVVLLATNQELKKFLEASVGYSPHLLYALIVDP
                     QGKTLLHSEGAKTERPHPIRPRLEDLLSMGPIDRFTVLYQEGSIFDSTLPMTWENVPI
                     GEVIVGIHTSLLRERMTSSLKTSVVFALVALPIAWLLTMGLATLTLRPIHALADQMEQ
                     LRQGRFDVLTDLSRTDEFRELASQLQLLGQQLKSDRLKTLSEETHLQGVIEHLEDGII
                     LIDAEGQILFLNQAMETMVDMPISPPPGRLDVLGESFSPLIQLVEQALTEKKNFKAVS
                     CFLPRRGERKSFYISIVCLGPFSQLHGAMILCRDIESMKTLQSLVRYAAQLTTLGQLT
                     SGLAHEVKNPLNSMVIHLEILKEETKGLNGDFQKSLEVLEGEVHRLDRVVLGFLKFMR
                     PQELELSSVNVNQLLKRELSLLEREWGKKGIRFVLDCESDLPVISADPDLLSQVFLNI
                     ILNACQAMEEQGGDILIATSTHQDNEIRISISDQGPGIPPEMQEKIFQLYYTTKHHGS
                     GLGLSLVYRFIQLHEGIIDVQTEMGKGTTFIVRLPRKV"
     gene            complement(16100..17479)
                     /locus_tag="PKM72_05675"
     CDS             complement(16100..17479)
                     /locus_tag="PKM72_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP60308.1"
                     /translation="MAKKIFVVDDEKASREGLAILLAKWGYEVEQAGDGKEALTKIPH
                     FHPDVVITDLVMPGFNGLELIQFLQKELIFSPVIVLTGHGTIETAVSAIKQGAYDYLT
                     KPVDVPRLRIMVEKALEKGETQREMVLLRKRLKGLWGLGKLVGKSKPMQEIYSLIELA
                     APTPARVLILGESGTGKELVAQSLHDLSERNKGPFIPVNCSAIPETLLESEIFGHEKG
                     AFTGALDRKPGCFELAHGGTLFLDEVAEMSPSIQAKFLRILQDSSVKRIGGTVPIQVD
                     VRVVAATNKDPLKAIQDGSLREDLYYRLNVCIIHLPPLRDRTDDIPLLVKALIEEFNG
                     NYTRTVQSINDEALERLMKHTWPGNVRELRNAVERAVMTCTADVITIAHLPEFAKAAD
                     EENPEANGEMVRLPMGATFEDAERQLIVQTLKHNQNNKTRTAEILGVSLKTLHNKLHR
                     YGLHDAIER"
     gene            18101..>20120
                     /locus_tag="PKM72_05680"
     CDS             18101..>20120
                     /locus_tag="PKM72_05680"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNP60309.1"
                     /translation="MDKKIVRIGDEHYILATSALADSRTHVLKDGETFGIFNPYGDIQ
                     HVGLGEQGLYHEGTRFLSGLEFHLEETHPFLLSSAIKGDNALLAVDLTNPDLDRTGFP
                     FLPRGTLHISRTKFLYQSRCLEELRFTNYSLDALHFGFSLYYQADFADLFEVRGEQRS
                     QRGIINISLLNDHTVSWQYQGLDHVQRTTHLTTDLAPQQITTSSMTFNLSLKPQEEKI
                     CHLSIACSINGKALEPKIFSTAQSHAAHNLQTTKSQFCTIYTGNEQFNDWLNRSLDDT
                     CMMTTDLPEGPYPYAGVPWYSTAFGRDGIITALECLWINPSLARGVLSYLASTQATEI
                     IPDQDAEPGKILHETRKGEMAALKEIPFGRYYGSVDSTPLFVLLAGAYYDRTGDKDFI
                     VSLWPHLERALTWIDLYGDKDKDGFVEYFRQTPTGLAQQGWKDSYDSVFHADGTIAEG
                     PIALCEVQGYVYAAKRAASHLARLLEYPERAQSLLREAQTLQDQFNRQFWSEELDTYI
                     VALDGKKQPCTIRSTNAGHTLFTEIATQDRAERIAKGLLEEDFFSGWGIRTIPTNEIR
                     YNPMAYHNGSIWPHDNALIAMGMSRYGLTQSVERLIGGLFDLSIYVDLHRLPELICGF
                     PRKPGEGPILYPVACAPQAWAAGAVFLLLQSCLGISFHGAEREIRFTHP"
BASE COUNT         5305 a         4883 c         4297 g         5635 t
ORIGIN      
        1 ggaatcacgt gaaccggctt tgggcattat tgttcattcc cattttttcc tcattcaatg
       61 tgtgttggtc tcatccaaat ggttacccgt ttcatttacg agtttggact ccagaatctt
      121 tagtcggtgc atccgtgctt gcaaatgtca tggagttgat aataaacgat agaccaaatc
      181 tcctcatcgg aaagcagatg cttaaaaggc atatgagatg tccccggtac ccccaattga
      241 ataatagaaa aaagttcttg tttggttaga ggtcgtcgca cggcggtccg gccatgacaa
      301 tgcatacacc tggcaacccc ctcatataag gctttgccct gttgaagtcg ttcttcggag
      361 ggtggcaggg cgtcgggttg atcaccattc agaatggtcc ccggataacc gggttcaacc
      421 actgagaggc acataatcat cacaaaggct agggttcctc cataaaagct ttgcttcatc
      481 cctccatctt taatcaatct catcagatta tttcctttct gaatcataca acatcaggat
      541 cgtcacatcg ccctcaaagg ttgttgttgg ccaccctggt ctgccctccc ttccacagac
      601 cggccagcgc attcatctgt ccggtccgtc aacattccgg gaacatacgg gacatttgtc
      661 atgtgagcgg ttgaaaacta acctttccgg ttccttttgc catcagtccg gaatctcaat
      721 ggagaagttt ccccgcgcaa caggaaggga accagttggc attcgaataa tttttcaagc
      781 aattcattgg ttactttata gaacctactt tactttagta actagttact tatcttaact
      841 tataaaaccg gccaaagaca aaaatggtta taatcttaag gaaggtgcac aatgggagaa
      901 tgcgaacgct gcagaggatt catggtgaga gacagtattt ttaacgtgga cggacagttt
      961 ttagaaattg aaatggggag atgcctcaat tgcggtcatg tcgtcgatct cgatttcctg
     1021 aaaacccgtc aaataccaaa cggtagaatt ccagcaaagc aggaaggggt gcttgcctga
     1081 gcgccacatc tttgtcattc caagtgcatt gcgtgaagaa cgcgaaaagg agttgccaat
     1141 gacagaaatc atcggaggga gtgctgccta ccagtatgta tggtcgggca tgatcatggc
     1201 cggttctctg tggggattgt tcttgagctc cagaatgatc ccgctattca cacgaagcct
     1261 cacgcgacat tccaggaata tatccggcgt gaaggatgtt ccctaacaac gcacttcatg
     1321 aattcgacgg gaaaagcaac gttgatttcc tcggggaaat taaatatttc aagagtctgt
     1381 gcgataccag ggaggtaatg atgaatatga ttcgtccgac gatccaacat aggatccacc
     1441 agcttgccgg aatgattgct cacgttggcc aggccgcgat tgtgagcatg atcctggctt
     1501 tttcgttggg gaccccctat ctgcttcagg ccgaaccggc ttcccaggct atgaccaagg
     1561 aacaggccat aaagctggga gaagacttcg gcatcatcgt cggagaagtg gacgaagaga
     1621 tacgagatta tctgggctta gagcgcgcaa aaggcgtggt agtctttgag gtaatcgggg
     1681 gaaaaccagc ggctcttgct ggaataaaac cccgggccct catcaaggaa atcaattccg
     1741 tcgaggtaac taccctgttg gatttcggac tggctctcca ggacgcgttg cccacagaaa
     1801 atttttccgt ggcaacgtat gaaccatctg accccgacaa ccagggcatc agtggcggaa
     1861 ttaactttca ctttgttcga gttgaaaaaa gctaaaccag cttccggtcc ttcttgaaat
     1921 cgtgagcgga attgccgcgt caaagccctg atgatgccag gacctccaag aagggagaaa
     1981 cacattcctt ttcgcattca tgaatttccc gacctctcga tctaatcggg tggagtcctt
     2041 tcccgcagga aactactcat cttggattaa atttcaggaa aagccgttca ggctacacca
     2101 gacacagatg tctttccacc cgtgttggat tctcttttca cgacatgtag ggcctggcga
     2161 cggtaaacac actttcgaga aagcgtttgg tcgaggagaa attcccatca ggcgactttc
     2221 cgagccgatt gaaagaggga atgtctcaca tccctccata tccggggaat ttcctcgtcc
     2281 aaccaatctt tggcggtagc aacgatcgcc taatttttac gtgtagttct tccctttttc
     2341 agaggaggca tcacggaatc gttagatacc gcctttctcc tttttgcttc tttcacctga
     2401 acgctggagt tcgccgtctt tgtctcctga tcctgccatc ggtcagccaa agtattgatt
     2461 tgatccagaa tggtggtgag ttcacgacct tccgtcgtga gtccataggt cacctggagg
     2521 ggaattgtcg atacttggcg acggaagata atttcagcct tctccaacga tcgcaatcgc
     2581 tccgtcaaca ttttcgatga aatgcccggc atttgacgct tgagctggcc aaaacgggtt
     2641 tctccattgg tatgcaagcg gcaaagaatg taaatcgtcc acggcccggc aagaatgcga
     2701 aggagagagt ccaacggaca accgaaagaa cttggtggca acttcatggt tacttaacag
     2761 tatctacttt acttttgtaa ctagttactt atcttaacca gcaggaaatc tcagatgcaa
     2821 gtcaacgtat ccaaaaggag gaaatatgtt catgacgact gatcagatga gtgcagagag
     2881 gacgatgaaa tgccaccggt gcgcaggact gttagtgcgg gatagcattt ataatctcga
     2941 tggtcagttt caccatcttg aagtgttgag atgtctcaat tgcggagaca cggtagatcc
     3001 caccattctc aaagcccgcg gaatgaaaaa tgggcagcga ggaagaatgg aactccagtt
     3061 ttcctcaggg cacgttcatt aaataaaaat gttcgacaga aaaggggtgg agcacagcat
     3121 aaaagcccag tggacaaaga gaccgggatg agaaggagta ttgatgcgtg cgggtaaaaa
     3181 ttgagaggga cgtcagccac aaagagttgc gctctgtgct cctagaagga ttcgggtccc
     3241 attgattctt gtgcgcaagt aaaaaaagaa cctcatggaa agtgaattgc cacaccaatt
     3301 ttttcagggc aggtgaagag gaaacgcgga ctaaaaactt attcattccc accgaatggc
     3361 tatttcaacc ttttcttcag accataaccc aggccgcagt tagagaaaac acattctttt
     3421 ctgagggaaa atcgaaagtt ccttctaccg aaaccctttc gccagcattg ttctctgtga
     3481 caaattgaaa ggtgccttta atcgtactgg cttctggaga taatcggtca ggtgaaaagc
     3541 tatccaacgt cattgtgccc tccgtattgt tttggtacgc aatggattgt cctttttcaa
     3601 ccgcttctat ctggacatca aaattctgac ctacctttag tggatcaggg gccatcaggt
     3661 tatagtgtcc aggctgggct ccatccggga tccggaatat aataaagaat tctctccctt
     3721 tcatgtcatc tctaggtaga ttgttcacga gcatataata ccctgggcga tttccggaaa
     3781 ccgtaccttc ctttggaggc agatatgtca caattccgtc tccccatatg tctgcatgaa
     3841 ccgcgccgtc tacatttgca atgaatgcgg aatgttcgct cctgggctcg ggagtactct
     3901 tatcggtgga tgattcagaa cacccgccga tgaaacctgt cgtgaagata aaacttaata
     3961 tgataaacct tgaaaaattt actatcatga cttttcccct tcttcgggtt ttccctagga
     4021 tgcagctact tacaccccta taatttccca tgattaggga attgataggt gaatgattgt
     4081 ttaatgccct cgttgtagtg caatcataaa ttcatcctga agacccggtt cactttggat
     4141 cgtttgaaag attaaattgt attcttcaac cgaaagccca tgcttcgcga cagcctgggt
     4201 cattttttgg tttgcttctt cctgcaacgc atcactttga cccggctcgg attgaagaat
     4261 ccgctgggca tatatttcac tgagctccga cacttctttg taagctccgg caaaaggttc
     4321 aagatttttt cgaaccattt cctccggtgc agattgagag gaaccatcgg cggcaggtag
     4381 aacggccgct ggcatcaata aacagacaaa ggcgaaacaa gccacaatac caaaattttt
     4441 aaggtttgat tgttgggatt tcataatctt ctccttgtag gattggttca tcataggtat
     4501 aaaaattttg aataccgctt tcaaacatac attccttttc ggtcaagcac tctttcttaa
     4561 ttttctatcc ccaacccggc tccggttagg gctcgaataa gatcatgtcg ggtcagggag
     4621 ccgatgactc gtccttctct tgtgattggt attatttgta attcttcgtc ctgaaataat
     4681 gtgacgaccg cagagatggg tgtcatttcc gttaccgtgg aagtcctatc ttctgtcatg
     4741 atgtcctcaa cttttacctg actaatatcc ttccccgaat acaaggcatt aaggatttct
     4801 gactctccaa ggaatcccgc taagtgccct tcgtcgtcga ccacaggagc caccaccaga
     4861 tgactggata aaatatccat aatggcggaa tttccctctg attgagccag atagatcgtc
     4921 atatgagttg gtacgatatc gccaactgtt gaataaccgg ttgcagacat tcccgctata
     4981 gtcgtcatca cgccccccct tctctaattt attcaaaaag ttttgttgat ccaactttgt
     5041 cgttctgacc catcctattt attttccaag caaaaggtag acccaaaaca tattccttga
     5101 gccacaatga attttctctt acttttcaaa atattaaaaa aatctggcga gacggaatct
     5161 aatttgaaaa ttttcaagag aaccacattg aagtaaaatt tacagaaatt cgagtaatac
     5221 ctacagactt gaatactgga atgaaaatca tgcatggagc atcacatttt ccggaatgta
     5281 tcatcattaa ttcacatgtt tttttgcgaa acgggctagg gatgggagat agcgaccatt
     5341 tgaagggtca caggggaaga aaaccttaat aactcttttt aggtctgatg agtattttga
     5401 gcaaggattg tcatccattt ctcgttaatt tttcatttct ttaaccaaaa tggacatctt
     5461 gtctgttttg aattccctgc ttgtagggcg gaccgtatgc cccctctatg atcattcatt
     5521 tccaacgctt cctagcacgc cttaacgaaa cacaacaaat tccctccatt agcgaaaacc
     5581 tttttcctgc aattttcaca aaataatttc ttttacctcg ttcgtgtaac ccttacacgg
     5641 gctttgtagt ttttacattt ttatttctgc ttagaactca ctgttgtttg aaatttccat
     5701 ttcattccaa caaaagtttc attcgctcaa tcacttagct ctgctctcaa taaaaaattt
     5761 tgtcggcatc gagtttgcta ataagcgaat acaacaaaga ttagtttccc ccggaggttc
     5821 ttttaatttt ttttcatttc ctagaaagga gtttcccatg agaatcgccc aactggctcc
     5881 cttggctgaa agcgttccac caaaacaata tggaggaacc gaacggattg tttcctattt
     5941 aaccgaagaa cttgttcgtc aggggcatga tgtcactctg ttcgcttccg gtgattctca
     6001 gacgaatgct catctgaaat ccatgtgtcg ggaaggcctt cgaaaaacta atggggtctc
     6061 caatcctcag gctcccttta ccatgatgtt ggagaagaca ttctcctccg cagatcaatt
     6121 tgaccttttt cattctcatc tggatttttt agcctttccc ttagcccggc gttgtgggac
     6181 tcccgtcctc accacacttc atggccgcct ggaccttccc gaactgaaac ccatttttgc
     6241 agaattttta gattgcccgc ttatttccat ttccaatgcc caacgtaacc ctctgacctg
     6301 ggcaaactgg aaacaaacca tctaccatgg attacccgaa aatctgtaca gctttcatcc
     6361 tgaatcaggt tcttacctcg tctttgtcgg gcgattaaca ccggagaaac gacccgatca
     6421 tgccattgaa attgccaaac gcgtcggcat gcctttaaga atagctgcga aagtcgatcc
     6481 tgttgaccag gaatattttc atacagtgat taaacctctt ctatcggatc ccttagtgga
     6541 atacgtgggt gaagtcacgg atgccgaaaa aaatgactta atcggcaatg cctttgcttt
     6601 ggtcgcaccc tttgattggc cagaaccttt cggtttggtc tttattgagg cattggcttg
     6661 tggcacaccg gtattagcat atcgtcgagg ttccgttccc gaaataattg atgacgggca
     6721 taccggattc atttgtgact ccatcgaaca catgattaaa gccgtgccct gcatcgcttc
     6781 tcttgaccgt caaccttgcc gtttggcctt tgaacaacgg tttaccgtta aacgaatggt
     6841 tcaagactac ctgagagcct atgaaaatct attgaacgtc caggaggaga actaccaccc
     6901 ttcattaaac ggtcgtgaaa cggctgtgaa tttctaacct ccgttgcttt gacacatatt
     6961 ttaggaacca ttcgtgtaga accaggaaca tcatggctac ttctaatcat ttggacacct
     7021 tacccaatag atgggatctg tttagaaatc gaaacttccg catgctgttg gcctctcagg
     7081 ccgtttcaca aattggggac gggttaacca aagtagctct tttatggttt gtgtaccaac
     7141 tgacgggatc agccttgaaa atgacgatca tcggtctatt gcaaacgatt cccccgctgg
     7201 ttctgagccc ggtcatcggt gtgtatttag atcgattgcc taaaaaacct gtgatggtaa
     7261 tccttgatgt cattcgagct tttttaattg ctcttattcc tgtcctgtat gtttttgatg
     7321 ccctgaccct ggaagtgttg tttattttgg tgtttttcac agctatcttc tctacggctt
     7381 ttggtccggc attggctgca tcccttcctc ttcttgtcaa gccctctgaa ctcatgtccg
     7441 ccaactctct tcttcaatcc acaacaaata tgggcatatt gatcggaccg gctatatgcg
     7501 gagtcttgat tgcatggctg ggcacccaga atgttctcta tgtagatgcg gcaacctttc
     7561 tgttatccgc cttgtgccta ttgccgatca aaggtcattt attgacgacc gctcccagga
     7621 tcgcgaatgc gacttcgaat atcagtcagg acctggctgt cggaatgcga tttgtgttta
     7681 gagaaaaacc cgtgcttcgc gctcttctca ttacaacggt cttttatacc ttgggagcca
     7741 gcgcctttat ctatgtgctt ccacttttcg tcaacgatca cattgaagcc gatccgagat
     7801 ggttggggtg gctgtggtct gccatggggg cagggatgct tgcgacctcc tttggtttgg
     7861 gttgcgtgaa gaagctggaa cggaagagtc ggaccttcct catggctttt tcaatggtcg
     7921 tgggcggaag tgcgctgcaa ttccttgctc attcacaatc ggtcatgtgg ggctttatcc
     7981 ttgtgaccct tattggggcc agtacggctg ccttcactcc cattgcctgg tctatcattc
     8041 aggaaacaac tcccaaccat ttaatgggac gagtgtttac cttaatcaat actgcgagta
     8101 tggccatggc cagcgggggc atggtggcct ttggaggggt ggctgataac ttcggatcaa
     8161 tggccggcat ctcgatgatt ggtttcgtat ttttgatcac aggcatggtc gtgatttttt
     8221 cgaatcgttc gcccattcag gcggaagagc ctctcccgtg gaaggcaatg gaatctcctg
     8281 accattctga ttttaaaagg gcttggcatc cagtcagaat gggaaattcc acttctcccc
     8341 tcgatagacc atggacaagg aaccttcaat ggatctcaat taagtcccca tatccattta
     8401 ggcaaatacc gtctgtccgc ccagcatctg gctcgcgcgc accgccggac cagcaaaagt
     8461 ttcgcatgag tcctcagacg ctcatctata agaatgaagt atatgaagcg attccgcctt
     8521 agaccacttt tgatattcct agaacaattt acacttagga gaaacttttc gagtccaatt
     8581 agcctcagag ggttccatcg tttcgacaag aaatctcaat cagaaaagct ttattgaaaa
     8641 ccagacaatc tgggtcaaag atttgtcgta tttcatgaat ccattttcta ccggagtcca
     8701 acctgaatga tttctgaaga aatatgcagc ttgaatggaa ttgagatgaa tcatgacaaa
     8761 tttcaacatg caaattatca ataaaatctt aatgaaatcg acttttacaa ttagttgtat
     8821 tatatggggg catatatggg ccataaatgt gactcatggc tatgatgtgc ccatttcagt
     8881 gccaggcgga actcatattc ttcgtgtcca gaatcatcaa acgaacccat ccccgaaaaa
     8941 agaattttct ggcgaagatc agcgaaaaac atcagccaac atcatagaat cccggaattt
     9001 ggaattaaaa ctggcgttta tgggcgatcc ccgtctcttt ccttttgata tcgattgcca
     9061 gatccaagaa aagacagtca aattgacagg ctcggtaact cttgaagaag aaaagacttt
     9121 ggctacgctc ctggcttcac acctgataag ggaaaaagag atcgttaatc tcatcgaagt
     9181 tcgtccctcc ttgagcgcaa ccttgcaggc cggcatggac actagactga cggaactagt
     9241 taaacagcgt tttgcccaaa gccaaacctt gcgggacgcc aacttcgaaa taatcacaat
     9301 tcgagggata gtatctctaa aaggccaaac ccgatttcag gtgattgcct tggaggccgc
     9361 acaagccgcc cgggaaattc ccggcgttat tgctgttctt acccaaaaca ttcgtcttga
     9421 ggcagggaat gagtgaagga actcatgtag aaagttatgg ccaaactttc tctatcttct
     9481 cccacccttc ttcaatcggt gtctattgca accaatcctc cgctcttgtt gcaaagaaat
     9541 tttggaatta tttggtggag tcagcttatc tctcaaatcg gagatggagt gaccaaactg
     9601 gcactattat ggtttgtcta tgcggtcacc ggttccgcgc tccaaacaac gatcattgga
     9661 atcctgcaaa cactccctcc tattgtgttg ggcccattca tcggcgtctt cattgaccgt
     9721 ttccccaaaa aaatcattct catcgggacg gatcttatac gagcactcgt tattggagtc
     9781 attccctgcc tggtctcaca tgaagcattc acggttgaat tcctatatgt gttggtattt
     9841 tgcaatgccg tagccacctc cttattcgct ccggccttat tcgcctccat tccgtttatc
     9901 gtggcgaaat ccgccttaac acccgcaaat gcactcctgc aaagcacaat gagtctggga
     9961 gtaatattcg gcccgttgtt gagcgggtta gggatcgccc ttttaagttc acaggaggtg
    10021 ctctgcatca atgtcttgac ctatattggg tcggccctgc ttttggtatt tgtttcaatt
    10081 cccgaggtga ctcatcgggc taaaaccttc caaagctccc tcaccaatta tgtaaaagat
    10141 ttacaagagg gcgtccaatt tacctttttc cagcagcccg ttattcttcc ccttatgctt
    10201 acggcgggac tgtacagttt tgtgatgagc gcctttcata ctttattgcc ggtctttggc
    10261 aggaacatgt tgaaatttgg gccggtggaa gtcggttatg tcacatcagg agtgggagtg
    10321 ggcctatttt tggcatccct attccttgcc aaggtcaatc taggagcaca gtggcgacgc
    10381 attcgtctat tagccacaac cagtcttatg gccgcttttg ctatatgggc tctcacagga
    10441 gccattgggc tgcctcttag tttggcaatc gttgttgtta ttggcatggg aagcggcgtt
    10501 ttgaccccga ttgagtgggg aattatccag gaaatctctc ctccacaact tataggacgg
    10561 gttttggcct tatatggaac cttggccatg ctctcggcta tcggaggaat gacgatgttt
    10621 ggatggatta tggaacagtt cggggaaatc tggacgcttc tgggcatggg ggcggtccta
    10681 ctcatcacgt ctcaggtcgc attcaaactt tcacgtagag tccgatccca ctcgaattat
    10741 gattagttgt tgagtgaaat cccttcctcc tgttcattcc tgtatcccaa agagatctcc
    10801 ttcccatgtc ccaacgacaa ccgggaaacg ctttcacttt ttaggagagg tgagttccca
    10861 cgatgaactg aagaggatag aattttgaat gttttttaga agaagagcca aaaagagatg
    10921 agggagcttg aggtccttac ccaaactttc ggaaaattca tcaatttcaa tcggaacttt
    10981 accccaaagg gaagatcctt catggcattt aaaaataact gaaaaaccgc atccatgtcg
    11041 ctggaattat tctgatcaac agcaaatcaa attatgttta ataccgtaaa tttttcataa
    11101 ttcatcaaac acttctacaa tttttaacga taaatcagag agagaacagg gtttttccag
    11161 aatggcataa acccctgcct gttcagcttt ttgtcgaacg ggcacactga tatctcctga
    11221 cacaaaaatt atgggtagat ctctgtgata ttgctggagc cagtcgatta actctaatcc
    11281 atccatttta ggcatgcgaa aatcagtcac caccatatca acatgatttt ttttgagttg
    11341 tactttggcc tcttcaccat tgcctgcttc aacacatatc agtcccaatc gtaccaacct
    11401 gtcacgtaaa agcaatctaa agtctttttc atcatcaaca accatgacag tttttcctgt
    11461 tgatcctttc cggggtaatg gttcccttat cgtcacgaaa tcatacccat tgaatatgtt
    11521 cccaagtaat tgtgtggtca tcgtgaccct ccttcattct ttcaatttcc ccaaatactt
    11581 cttccataaa agaattccat tcaggaatac caaatttact ctttgaatct catagaacca
    11641 ttccaattaa agattattct ttaaatgtgg ccatttacat aactttcggt gccggaacat
    11701 cgacgcacat cctccgaatt tttccaccac aaaggggttc atcagaaccc ccgataccca
    11761 gacaccgttt tgaacctttc ataaataaat atttgagaca acatattgac gccttagtaa
    11821 gagcttatcc ctggttcatc ctgaaattct tggatcttaa tagcataccc ggcgtttcct
    11881 aattgagcga taatggtgct cccttcataa atgttccctt caagagtgct ccgttcatca
    11941 agatgtaaaa tttcattatt ctcaccctga tttacaacta gaaagtttcc atccactcgt
    12001 tcgactcttc cgctaatctg acgcggcaaa acagaattat ttgaatacgc attattctgc
    12061 tcaattttct gaacggtttg aatcgttcca ttcctttcaa taattgcctt cacacgatcc
    12121 cctaaaaaga tctctccaat tttctgtgtt ttttctccaa gatggaggtc ccatactttc
    12181 cctaacgggg tttccacttt gtatcgccga tccttcattt gagaaaaatc ttttcccaca
    12241 aattcccctt ggatccttgt aacctctcct tcaatggtga tttgcccgat gtctccttgt
    12301 ccttgaatca catctttcct tggagcatcg gaaaacatcg ccatatccgt tgaaggattc
    12361 aaacccaaca ctccgaccat gacaatcacg gaaacaccta tcgcaatatg aaaatgattg
    12421 aaattttgtc tcttcatggc cctccccctc ttcttcaccc cactcaacct tcattcggcc
    12481 gagaatcgga gaaaacttct ctgcgatctt tccccaattc catagaaccc ttactcgttc
    12541 atgagcactt tcactttctt ggcatgacca tctccctcta ccatggcgat aatcgttacc
    12601 cctggcagaa cacgaccgat ggtgtcggta tacttatcca cttgaatctt ccattcagtc
    12661 ccttcaaaat cttccacaac acaccaatcc ccctgtacgt cttgaacagt cccgcttacc
    12721 atggtcaaaa gttctttctg atgcgcttgt tcggtcaagt cattggtcgt tccccgtact
    12781 tcggatgcct ctgcaatcgg actgacccaa atagaaatga aaaaccctat gacgaaatac
    12841 gaaaatattt tcatgagatc ctccttcggt tatagggcgt aatttgaatt ccctcccaac
    12901 cctgcccagt tttttcggga aagagggtcc tcaatctaaa aaagctatac cgcaactttc
    12961 aggccagaac agtcctgtgg acttattcaa gaaaaaccct ggagaaatcc cgaatcctgg
    13021 ggtaggagta tcggccgcat cagagaggac catgtaaaaa gtcctcgaac cggtaaaaat
    13081 tacaaaaagt tgaaattttt acaaagcaat gagaaaaaac ctctgatgcc gcagatacgg
    13141 ttttagaaga aaagaaagat tgaactaacc aaaaggagga ggggggacaa ccggaaggaa
    13201 gagtgtcaag agcctggaac tgactattca gcccccgaga gtttccatca gagatttaag
    13261 atgggcaggg cgaatgggtt tttccaagca tccagccaaa ttgaattgtt gagcgcgagc
    13321 ctgaatccct ttttctccac tgacgacaat gatgtctatg cttgggttaa atgatctgaa
    13381 aatccggatc aaatcccagc cattaatttc cacattcatg acaggaggaa ggttgatatc
    13441 gatgacggcc acttcaaaga aattctcttt gattttctta atagcctgca agccgtcttg
    13501 ggaagtatcc gcatgatatc catacattct gagcatagac tggaggccct cacggctatt
    13561 cagatcatcc tctacgatta acacgttttt ttttgtcatt cgacaccaat ttgggaggca
    13621 tcctcaccag caagaaagga atttcctgtt aattgccaat attcctcttc caacattgga
    13681 cttctctccc gataattctt caaacccatt tcttgctacg gttttgtgga attattgaca
    13741 atttctttag tcaaagtatg ggctttctcg gatagattca atgccatgga cagatcattt
    13801 tgtgaaaaag cctcttgggc tttttccaga aaactattaa tagaaatgta ggtatcgtgc
    13861 tgttccttgg acagttggct ttcattaatg gtacgcaaca aattttgcgt ctcatctatt
    13921 tttgacttgt aggtcacaat ttcctgacga ttatccttat taggaatctc ggggacaaga
    13981 attgccggtt tttgttcttg ggaatccggg acgtgttgtt gcggtttatc ggatgggatt
    14041 tcatttttcc cggggatagg ctcggaagac atgcggtcat ctacagaaac tggcctgatg
    14101 ggagatgaat tggagaaaag atgaaggaat gacggacgaa gggcctctat ccctggtgtg
    14161 atctgacggg gataccaaag gtccgttcgt gagacaacac tgtctaatga gcatccacta
    14221 agaaacacag tcattccgca tatcagaaat tgacctccac ccttgaagat cctccgttga
    14281 agacatgacc acaaaaacgt cacctcaaac ctttcttggc agtcggacaa taaaggtagt
    14341 gccttttccc atttccgttt gaacatcaat tatcccttca tgcaattgaa taaaccgata
    14401 gactaacgaa agacccagac cgcttccatg atgtttggta gtgtaatata attggaaaat
    14461 tttttcttgc atttcaggag gaataccagg accctggtcg ctgatggaaa tccgtatttc
    14521 attatcttga tgggttgaag tggcaattag aatgtccccg ccttgttctt ccatggcttg
    14581 acaggcattt aggataatat taagaaaaac ctgacttagg agatcgggat ctgcggaaat
    14641 gactggcaga tctgactcac aatccaggac gaagcgaatg cccttttttc cccattccct
    14701 ttctaataga ctcaactccc tcttgagcaa ctgattgaca ttcacggagg aaagctccag
    14761 ctcttgggga cgcatgaatt tcaaaaaacc aagtacgaca cgatccaaac gatggacttc
    14821 cccttccagg acctccaaac ttttctggaa gtctccattg aggcctttgg tttcctcttt
    14881 taaaatttca agatgaataa ccattgagtt cagaggattt ttgacctcgt gggcaagccc
    14941 tgaggtcagt tgtcccaaag tcgtgagctg ggctgcatat cgaaccaacg attggagagt
    15001 tttcatggac tctatatctc tgcataaaat catggctccg tgaagctgtg aaaatggccc
    15061 caaacacacg atggagatat aaaatgactt acgttccccc cttctgggta agaaacagga
    15121 cacggctttg aaattctttt tctccgtcaa tgcctgttcc accaattgaa tgaggggaga
    15181 aaaagattct cccagcacgt ctaggcgacc agggggagga gaaatcggca tatccaccat
    15241 cgtttccatc gcctggttca gaaagaggat ttggccttct gcatcaataa ggatgattcc
    15301 atcctccaaa tgttcaatga ctccttgaag atgcgtctct tcacttaggg ttttgagacg
    15361 atcagatttc aattgttgcc ccaacaactg gagttgcgac gccagttccc gaaattcatc
    15421 cgtccgactt agatccgtta acacatcaaa acgtccctgg cgaagttgct ccatttgatc
    15481 agccaaggca tggataggac gcaaggtcaa cgtcgccagg cccattgtta acaaccacgc
    15541 aatcggcaag gccaccaggg caaatacaac actggttttc agggaagacg tcattctttc
    15601 acggagcaga ctggtatgga tccccacaat gacttcgccg attggaacat tctcccaggt
    15661 cattggtagc gtgctgtcaa aaatggatcc ctcttgatac agcacagtaa aacggtcaat
    15721 gggtcccatg gaaagaaggt cttcgagtct gggcctgatc ggatgcgggc gctcagtttt
    15781 cgcgccttcg gagtgcaaaa gggtttttcc ttgaggatca acaatgaggg catagaggag
    15841 atgcggggag tatcccacgc tggcttcaag aaatttcttg agttcctgat tggtggccag
    15901 taagacaaca ggatgggtat cgggtgactg agcattcagt accacgcgct tactatactc
    15961 aaaaatttgt cgttttatga gagaaacctg ctccgaggtt tcctgaacga tgacacgact
    16021 taactgagaa agattaagaa acgtgctgcc aaccaccacc accagtgtaa ggactgtaac
    16081 ccctaatgct tcctttccct taacgttcaa tcgcatcatg taacccgtac cggtgaagct
    16141 tattgtgaag cgttttcaga ctgaccccaa gaatctccgc ggttcgcgtc ttattatttt
    16201 gattatgctt aagagtctga acgatgagtt ggcgttcagc atcttcgaac gtcgctccca
    16261 tgggcagtcg caccatctcc ccattcgctt caggattttc ctcgtccgct gctttggcaa
    16321 attcaggaag atgcgcgatc gttatcacat cagcggtaca ggtcatcacg gcacgttcaa
    16381 cggcatttcg caattcacgg acatttcctg gccaggtatg cttcatcaaa cgctctaatg
    16441 cttcatcatt aattgactgg acggttctcg tataattgcc attaaattct tcaattaagg
    16501 ccttcaccaa taaaggaata tcgtccgttc tgtctcgtag gggcgggagg tggatgatac
    16561 acacattcaa acgatagtac agatcttcgc gcaatgatcc atcctgtatg gccttcaatg
    16621 gatccttatt cgtggccgcc acgacacgaa cgtcgacctg gattggaacg gtaccgccaa
    16681 tccgtttgac tgaggaatct tgaagaattc tcagaaactt ggcttgaatg cttggactca
    16741 tttcggccac ttcatccaga aaaagggtac ctccatgtgc caactcgaaa cacccgggct
    16801 ttctatccaa ggctcccgta aatgcacctt tttcatgccc aaatatttca ctctccaaaa
    16861 gggtttccgg gatggctgaa caattcaccg gtataaacgg tcccttgttt ctttcagaca
    16921 aatcatggag actctgggca accaattctt ttcctgtgcc actttcaccg agaatgagga
    16981 ccctcgccgg cgtcggagcc gccagctcaa tgagactata gatttcctgc atgggtttac
    17041 tcttgcccac caattttcca agtccccaca aacctttgag ccttttccgc agcaaaacca
    17101 tttctctctg ggtctctccc ttttccaagg ctttttccac cataatgcga agcctgggca
    17161 catccaccgg cttagtcaga taatcgtagg caccctgttt gattgccgaa acggccgtct
    17221 caatggtccc atgaccagtt aagacgatga ccggactgaa tattaattcc ttctgaagaa
    17281 actgaatgag ttcgagtcca ttgaatccag gcatcaccaa atccgtaatg actacatcag
    17341 gatgaaagtg agggatttta gtcagagctt cctttccatc cccagcctgt tccacttcat
    17401 agccccattt ggccaaaagg atagccaacc cttcacgaga agccttttca tcatcaacca
    17461 caaatatttt tttcgccatt atgcatctcg cagtggattc agttgatcat gactcttgat
    17521 cattctccat cccaaaagcc atgccttctt aaagaaggca atttaataga tattaaaatt
    17581 atatccttta gcaggagaat cttcaccaaa aatgagcggg gcccctatcc cacaattgta
    17641 aagattccct tggtaagaaa aaatggattt aacttaactt tggatggcga aaatttccca
    17701 gccattaccg cctcccataa gagataagag tccagaaacc aggtaaggca tcgtggattc
    17761 ggcttgttcg atttcctccc aggattctgc cctcaaaata gtacatagaa actttatgaa
    17821 tcgatgcggt tttttgggga agatcagcca tgaaatcctt gaattcctgc cccagctacg
    17881 gaagaaaaaa agagatcgca agcgttccta atcccttctc gaagataaac aaaggcttcc
    17941 tgaaggaatt cctatcccac tcgatataaa attccggtga atcacgcatc cgaagatttg
    18001 caggagattt tcagatgtta tttcctctgg ttttgaaaat gtccctccgg actaataaaa
    18061 tgagtaatac aatcactttt cgctaatcaa aggttacaaa atggataaaa aaattgtccg
    18121 gataggagat gagcattata ttctagcgac ttctgcgctt gccgattccc gaacacatgt
    18181 cttaaaggat ggagagacat ttggcatttt taatccatac ggtgacattc aacacgtggg
    18241 tctaggtgag caaggccttt atcatgaagg aacccgtttt ctttcaggat tggaattcca
    18301 cttggaggaa acccacccct ttctgctcag ttccgcgatt aagggagaca atgccttact
    18361 cgcagtcgac ctgaccaatc ctgacctgga tagaactggg tttccattcc ttcctagagg
    18421 cactcttcat atttcgcgga cgaagtttct ctatcagtcc cgttgcttag aggaactccg
    18481 gttcaccaac tattcattag atgccttaca cttcggattt tccctctact atcaggcaga
    18541 cttcgctgac ctcttcgaag tacgagggga acaacgatct caacgaggga tcatcaatat
    18601 ttccctcctg aacgaccaca ctgtttcttg gcaatatcag gggttagacc atgtacaacg
    18661 gacgacccat ctgaccaccg atctggctcc ccaacaaatc acgacaagtt caatgacgtt
    18721 taatttatcg ttaaaacccc aagaggaaaa aatctgtcac ctctcaattg cctgttcaat
    18781 aaatggcaag gcattagaac ctaaaatttt ttccacggcc caatcccatg cggctcataa
    18841 cctacaaaca actaagtccc agttttgcac aatttatacc ggaaatgaac aatttaacga
    18901 ctggctcaat cgatcccttg atgacacctg catgatgacg accgaccttc ctgaagggcc
    18961 ttatccctat gcgggcgtgc catggtacag cacggctttc gggagggacg gaattattac
    19021 cgcgttggaa tgtttatgga ttaatccaag tcttgcacgc ggggtcttga gttatttggc
    19081 ttccacacaa gcgacggaga ttattcctga ccaagatgcg gaacctggga aaattttaca
    19141 tgaaacccgt aagggtgaaa tggccgccct caaagaaatt ccgttcgggc gatattacgg
    19201 gagtgttgat tcgaccccat tatttgtcct cctggctggt gcctattacg atcgaacggg
    19261 agacaaggac tttatcgttt cgctctggcc tcatctggaa cgtgccctga cgtggatcga
    19321 tctttacggg gataaagaca aagatggatt cgtggagtat ttccggcaaa cccccaccgg
    19381 cctcgctcaa cagggctgga aggattccta tgattcggtg ttccacgcgg acggcaccat
    19441 cgccgaggga ccgatcgctc tctgtgaggt tcaaggatat gtgtatgcgg caaaacgggc
    19501 ggcctctcat ttagcgagac ttcttgaata ccccgagcga gcacaatctc ttttgcgtga
    19561 agcccagaca ctccaggacc aattcaatcg gcaattttgg tcggaggaat tagataccta
    19621 cattgtcgcg cttgacggaa aaaaacagcc ctgtacaatt cgatccacca acgccgggca
    19681 tacgctcttc actgaaattg ccacacagga tcgggccgaa cgcatcgcga agggattact
    19741 tgaggaggat tttttttccg gctggggaat ccggacaatt ccgacaaatg aaatacgata
    19801 caaccccatg gcctaccata atggatccat ctggccccat gacaacgccc tcattgccat
    19861 ggggatgagt cgttatggat taacacaaag cgtggaacga ctcataggtg gactctttga
    19921 tttgagcatt tatgttgacc tccatcgcct ccctgagctg atttgcggat ttccccgaaa
    19981 acctggagaa ggtccgattc tttatccggt cgcctgcgcc ccccaggcat gggcagcagg
    20041 tgcggtgttt ttactgttac agtcctgtct gggaatttct ttccatggtg ccgaacgtga
    20101 aatccgattc acgcatccct
//
LOCUS       DAORAQ010000039        19930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_489_length_19930_cov_72.033258, whole genome shotgun sequence.
ACCESSION   DAORAQ010000039 DAORAQ010000000
VERSION     DAORAQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 19930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19930
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_489_length_19930_cov_72.033258"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKM72_05685"
     CDS             complement(<1..184)
                     /locus_tag="PKM72_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60310.1"
                     /translation="MKIREATIAEAQHSLAAPSRISWRIAAGLRRALLTVLVLIQSFV
                     AASYMTAILPYHGGNFL"
     gene            complement(181..429)
                     /locus_tag="PKM72_05690"
     CDS             complement(181..429)
                     /locus_tag="PKM72_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60311.1"
                     /translation="MARSIAEHTLSRVCDYLSAMGVELTREVTLRALTLVEAGLASEQ
                     EDPLQFVMTRIHDHFALQIPELPTTAPPITRGSMSFKP"
     gene            complement(429..1970)
                     /locus_tag="PKM72_05695"
     CDS             complement(429..1970)
                     /locus_tag="PKM72_05695"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HNP60312.1"
                     /translation="MRLLFAGMIAMFLMFPLHVAAGFTFDQVVQRAKALADKPYEAPQ
                     PIPKIMREISYEAYQGIRFNPEHSLWKESNSNFQVMLLTPGLHYTHPVTLNVIDSEGP
                     RPLVFKKSDFVFADPEIEKRVPADVGFAGFKLTFPLKNKNEQNQFLVFAGASYFRGVG
                     KENVFGLAGRGLAIDTGLPSGEVFPSFTEFWLVRPSPDAKEMVVYGLLDSISLTGAYQ
                     FTIVPGSQTKMKVRTQLFPRKPIQLLGVAPLTSMFFYGENTPRPAGEWRREVHDSDGL
                     QIHDGLTREWLWRPLMNPANLEMDFFSTENVQGFGLLQREENFSGYEDLGAHYEKRPS
                     AWVEPQGDWGQGKVVLVQLPTPNETHDNIVAFWTPVAPVTRETPLEMAYDVSFGKATL
                     SQNPLGTVVNTFIGDGNRIGGGNVPQAYRIIIDFAGGPLSKMPPDAPISGQVTVLDNS
                     DILEHFVEYHEQIHGWRLSILAKPQDKKPLHLRAFLKTDLATLTETWTYRLPTNNNIL
                     AQDKE"
     gene            complement(2771..3844)
                     /locus_tag="PKM72_05700"
     CDS             complement(2771..3844)
                     /locus_tag="PKM72_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNP60313.1"
                     /translation="MLRQLIPMIYLNPAGLAPFHQEVQQEISRTLDTFSHLLYADAGI
                     QYYRDTLQRCRRTIADWLTLNDEQRLAFMPNTTTACSLVLSRINWKSGNVLLTTTHEN
                     STILDEIKKLSDRGVRVIALDPDAPSGFLSALERTLDEQPIQAIVISHVSHFDGRIFP
                     ITTIQDLAQSHHTPLIVDGAQAIGHIPVSFQEISPYAYFFPGHKWCTGPMGTGALIIG
                     KDSHQETQPYWAGYELGTQNIGLIAGFAKACHLKVQTHSNNQILEGFREEWKACLRPN
                     PCIRTIEWDGPHAPGILSFVCLDERTEQTMHTLSTTQSLAWKTLTHPSFPSNLSIRVS
                     WTTDTPDIDIRSTLALFTSLSNK"
     gene            complement(3913..4896)
                     /gene="egtD"
                     /locus_tag="PKM72_05705"
     CDS             complement(3913..4896)
                     /gene="egtD"
                     /locus_tag="PKM72_05705"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNP60314.1"
                     /translation="MTSQTDPSITIDLPLTREDPDLLKTEITRGLLANPKTLPSKLFY
                     DERGSTLFEQICELPEYYQTRTEHQLLTRWADEIVGISEAEELVELGSGAATKTRVLL
                     DAMANVNQLRYFVPFDVDEAIVRRVSEELVREYPGLHIHGVVGDFLVHLEHIPEGGKR
                     LVVILGGTIGNLPATAAHEFLSSVNSEMAPGDYFLLGVQLITDRNRLEAAYNDQQGIT
                     AKFNKNIIRVLHNQFGSQSDSESFDHVARYNEADHRIEMWLRSREDQTLGIQDLNLKV
                     HLKQGEEIRTEISTKYDRPLAEELLASSGFALVKWYSDPDSLIGLALAKKP"
     gene            complement(5289..6884)
                     /locus_tag="PKM72_05710"
     CDS             complement(5289..6884)
                     /locus_tag="PKM72_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNP60315.1"
                     /translation="MTTYRIRQRRGRISIGFVVGLLGLLYSFSLPVWASDTIVVGSKN
                     FMESRLLAEMFAQLIEARTELTVTRRLGLAGTQVCFEALKTGGIDLYPEYTGTGLVTI
                     LGEPPMNDRVSVLNHVRTEFLHRWDIWWMAPLGFDNSYALALRRDQAQTLGLRTISDL
                     THVAPQMKAGFGYEFIQREDGLVGLNQSYGLQFQEVIGMQQTLKYQATDKGEVDILDV
                     YTTDGRLAVYDFVVLKDDRHFFPPYEAAALVRGEILNRHPELGKVINLLTNALDLPHM
                     RELNLRIQEQGEPVPQVAHSALRALNLVAGNHTQPDASADSSDSIFSYMWAKRSNLAM
                     RTGEHLGLSGFGLFLGILVAIPLGLALEQWRKGAETIIRIVGMLQTIPSIALLAFMIP
                     IFGVGALPAIMALWMYSLFPILRNTYSGLRDAAPNVVETGRALGMTEWQILRTIRLPL
                     AAPTIMAGIRTSAIWTVGTATLAAFIGAGGLGVPIVAGLQLADVNLILSGALPATVLA
                     LTVDGVLGRVEQWARPRGLDPVH"
     gene            complement(6895..7644)
                     /locus_tag="PKM72_05715"
     CDS             complement(6895..7644)
                     /locus_tag="PKM72_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNP60316.1"
                     /translation="MMPWALEIQQVSKQFPDGTYALQNIEWHVKEGETMALIGESGSG
                     KTTFLRLLNRLIEPTDGTVVIQGKPACLQDPIHLRRRLGYIPQDGGLFPHWTIKQNVC
                     LVPHLLGWTSEQQLNRVDMLLSLVNLDPSQIAERYPIELSGGQRQRVAVARALAADPP
                     IVLLDEPFGALDPLTRHDLQDQFLSLQIKLRKTMVLVTHDMSEAFRLGDRITILKEGH
                     IQQMGTPHEILHSPATPYVHLLVQHYHAHRG"
     gene            8409..8867
                     /locus_tag="PKM72_05720"
     CDS             8409..8867
                     /locus_tag="PKM72_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60317.1"
                     /translation="MKVNTLIGHDVGSIDLKTKNLNGRHPELAPDLPESGKRILVLGV
                     DLKTWIGLTDSLWDLGFTLKRLPSSLQELDQLKDAFVDGVLWDFESSSLKGLAVVSQL
                     RGGSPVLPVIVIASSSNKELLIKAIENGAMDFIKPIDHTEFRNKCVRLFG"
     gene            8912..9400
                     /locus_tag="PKM72_05725"
     CDS             8912..9400
                     /locus_tag="PKM72_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60318.1"
                     /translation="MIPLWVADSQELSPENPSISVQSKEKFDGKDPESPNAVSILVVD
                     DSVECRMILRIAIEREGYHCVEAADGVAALKVFQEKTIDFIITDFEMPHMNGCEFLEI
                     LSSSAISCPPAVMITGNLEDSVRIRATHAGALAVLLKPFDLRKFLEMVREVVHRKQEA
                     HQ"
     assembly_gap    9417..9516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9517..11384
                     /locus_tag="PKM72_05730"
     CDS             <9517..11384
                     /locus_tag="PKM72_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP60319.1"
                     /translation="QELKRTLHDLRERVKELTALHSTTQLFHNEDLSTQELLQHIADL
                     IPPSWQYPEVTAARIRFGHLEITTPNFVLSPWTQREDFICRDGTTGEIEVAYLEERPT
                     EVEGPFLQEERNLLHSLGEMVRLQLDRKRTQAALREAHDVLEQRVLDRTSELTTANLL
                     LSREVAQRKEAEAKLEKLIAEIQKNHDDLIAILNQLHIGTALTDQDGKVTFLSQAAQQ
                     LCGKANFPHSGEPWQALFDDPSYHAQIEDMVNRPTGERERILVRMPAVGGECTRWINV
                     DVRDDPRDPERKMFFLYDASEVQTLRQLLGETGKYQDLIGTSHSMQLVFQQIQDFSRV
                     DSTILIEGETGTGKELVARAIHQASHRNAFPFIAVNCAGLTESLVASQLFGHKRGAFT
                     GAVSDQQGLIEAAEGGTLFLDEIGDIPLTVQTSLLRVLQEWEITRLGESRLRKVDVRV
                     LAATHHNLANDVTAGTFRADLLYRIRVGRVLLPALRERREDIPSLISHFLNQYRATTG
                     RPVEAVSNEALRLLMAYAWPGNVRELKHAIEFAVIRCPQTIIRPQDLPPEVLNQDESL
                     LVIPSEERTPTHEKEKILSALKETGGNRSAAAKLLGIARLTLYRRMSRLGISTRR"
     gene            11878..12249
                     /locus_tag="PKM72_05735"
     CDS             11878..12249
                     /locus_tag="PKM72_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60320.1"
                     /translation="MNKNIFIPFILMGMLLGLPQFVLASEGSQEGNSSWKLEMQDWSI
                     QDHMAAAKDKEAEAQSLRSRVRHLGERIAHFEKKPQFDPKGMQRNSMKLIASTLTEEL
                     NRLNKEIAWHFRQADHAMLIE"
     gene            13162..13533
                     /locus_tag="PKM72_05740"
     CDS             13162..13533
                     /locus_tag="PKM72_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60321.1"
                     /translation="MNKKILFPLFLMGMLFGLPQFVWAAENSQEQSSAWKAETQGWSI
                     QDHMTAAKAKEGEVQDLQSRVQQLEARIAHFEKKPHFDPKGMQRNSMKLIASTLKGDS
                     EILNQRIAWHNRQSDQASLRE"
     gene            14361..15908
                     /locus_tag="PKM72_05745"
     CDS             14361..15908
                     /locus_tag="PKM72_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP60322.1"
                     /translation="MKFEMFHQTDQVGRHFYQTLLEVTNVLNSQRNTEDLWKAITGHI
                     KKVISWERAGITLYHHDIDAFRFYAVETSMPVVKLKCDAIIPREGSAMGWVYGHRRLH
                     IRPHLQRNPVFLEDAYYQEEGLGRMINIPLIVGDNCFGSLNIGSVESGEPDPENVLFL
                     RQVATQIAFAVDLVRAYEEINHLKEQLTRENAYLLEEIKVSHDFGAMVGNSQKFKNVL
                     DLAQAVAPTTTSVLIMGETGTGKELLARLLHELSPRKDKPFVRVNCAALPSGLVESEL
                     FGHEKGAFTGAENGRPGKFELAHNGTLFLDEIGEMPIEAQAKLLRVLQDGIVERVGSI
                     EGKDVDVRIIAATNSDLSKAIAEGRFRSDLYYRLHVFPIVVPPLRERLHDIPLLVRHF
                     MEKNRLQFRRNCQDIDDQSMELLIQYPWPGNVRELKNVIERAMILSQSTLVHVEESLL
                     PSPGHGMDALPPTQLKDVERTRILQALTACEWRIDGPLGAAKHLGLHPSTLRSRLKKF
                     GIKRQSE"
     gene            complement(16095..16835)
                     /locus_tag="PKM72_05750"
     CDS             complement(16095..16835)
                     /locus_tag="PKM72_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP60323.1"
                     /translation="MIKLTKPSLYHVALKKRQAIATGTMAFYFDRPPGFTFTAGQFID
                     LSLPNLSKADPEGHTRALTLASSPSEQHLMVATRLRDTAFKRTLTDMSIGASIDIEGP
                     FGQFILPTDDSRTIVFLAGGIGITPFRSMLVESAHHKLSHRIILIYSNRHPKDAAFLD
                     ELQTLQKENEHFTCIGTMTSPNRGTEIWEGETGRIDQVMLKKYVKETEKALYYVVGPP
                     AMVNGLRKMLESAGIPKTNIRSEEFVGY"
     gene            17022..18557
                     /locus_tag="PKM72_05755"
     CDS             17022..18557
                     /locus_tag="PKM72_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP60324.1"
                     /translation="MERIERNYRALLNVAIVLNSQRDTSSLWQAIIEQIEQVMPWARA
                     SVTLYDRKSDGFQFYVVTTHLARVVVQGDTVIPRVGSGMGWVYDHKTLHVRPDLNQEQ
                     VFLEDRWYVEEGLGRMINLPLLVRGKCVGVLNLGSVDAGDPDPDAIEFLSQVAMQIAY
                     AIDHVQAYEEINLLRQHLARENVYLVEELKLTKHFGAMVGRSQVFQHVLELAREVAPT
                     TATVLITGETGTGKELLAQAIHDWSLRAKKPFIRVNCAALPAGLVESELFGHEKGAFT
                     GADTRREGRFELAHGGTLLLDEIGEIPLETQAKLLRVIQDGMVDRVGGKQSIPVDIRL
                     MASTNADLPSAIEQGRFRADLFYRLHVFPITIPPLRARPEDIPELAQHFLEHYAIKLK
                     RPCELIEPDSLQRLIRYSWPGNIRELENVIERAVILSHQKVLRIDERLLILHNTTQES
                     KAPAGLVDFERQHILQTLASSNWQIEGENGAAEQLGLAPSTLRSRIKKLGLQRPNGST
                     PRK"
     gene            19387..>19930
                     /locus_tag="PKM72_05760"
     CDS             19387..>19930
                     /locus_tag="PKM72_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNP60325.1"
                     /translation="MKFYLDTVSLTEIREIGSLGILDGIAVNASLISEQGLNFYQGIR
                     EICRYVDSPISVGVLSIEEEAIVKEGIELSKIHRNVLVKCPLTPAGLKATKRLTAEGL
                     RVNVSLCFSLTQALIAAKAGAWCVSICPDHTGDNQSMGNDITRKIVTVFRDYGLSTQV
                     LLAGIRSPHEVLEAALAGGHI"
BASE COUNT         5069 a         4303 c         5199 g         5259 t
ORIGIN      
        1 ccaggaaatt ccccccatga tagggaagga tggccgtcat atacgaagcg gccacgaaag
       61 attgaatgag gactaatacg gtgagcaggg cacggcggag tcctgcggca attctccagc
      121 ttattcggct gggggcggct agcgaatgct gggcctcggc gattgtggct tcacgaattt
      181 tcatggttta aaactcatgc tgccgcgtgt gatcggtgga gcggtggttg gaagttcagg
      241 aatttgaagg gcaaagtggt catggattct tgtcatcaca aattgcagtg gatcttcctg
      301 ttctgatgcc aatccggctt caaccagagt gagagcccgc agggtgacct cgcgagttag
      361 ttccactccc atggccgaca ggtaatcgca gactcgtgaa agagtatgtt cggcaatcga
      421 tcgggccatt attctttgtc ttgtgccaga atgttattgt tcgttggtag gcggtacgtc
      481 caggtttccg ttaaggttgc caaatcagtt ttgagaaatg cccgcaaatg aagcggcttt
      541 ttgtcttgag gtttggccag gatggacaat cgccatccgt gtatctgttc atgatattcc
      601 acaaaatgct ccaggatgtc gctattgtcc aacaccgtga cctggccgct gatgggagca
      661 tcgggcggca ttttgctcag tgggccaccc gcaaaatcga taatgatgcg atacgcctgc
      721 ggaacatttc ctcccccgat acgattgccg tctccgataa aggtattgac cacggttccc
      781 aacggatttt gtgagagagt ggccttccca aaactcacat cataggccat ttctaaaggg
      841 gtctcccggg ttacgggcgc gacgggtgtc caaaatgcga cgatgttgtc atgcgtttca
      901 ttgggggtag gcagttggac gagcaccact tttccctgtc cccagtcacc ctgtggttcg
      961 acccaggcgc ttggacgttt ttcataatgc gcaccgagat cttcgtagcc ggaaaaattt
     1021 tcctcacgtt gtaataaccc gaatccttgt acattctccg tgctaaaaaa atccatttcc
     1081 aggttggcag gattcattaa ggggcgccac aaccattcac gagtgaggcc atcatgaatc
     1141 tgcaatccgt ccgaatcatg gacttcccgg cgccattctc cagctggtcg tggggtattc
     1201 tcgccataaa aaaacatgct ggtcagcggg gcaacaccta ataactgaat aggttttctg
     1261 gggaagagtt gagtgcgtac cttcatcttc gtctggctac cgggaacaat ggtaaattga
     1321 tacgctccag tgagactgat gctgtccagt aacccataga ccaccatttc tttggcatcc
     1381 ggagacgggc gtaccaacca gaattccgtg aatgaaggaa atacctcccc gctggggagt
     1441 cctgtatcaa tggccaggcc tcgtcctgct agcccgaaca cgttttcctt tccgaccccc
     1501 cgaaaataac tcgcccccgc gaaaaccaga aactgatttt gctcattttt gttcttcaag
     1561 ggaaatgtca gcttgaatcc cgcaaatcct acgtcggccg gcacccgttt ttcgatttcg
     1621 gggtcggcaa aaacaaagtc gcttttctta aaaaccagcg ggcgtggacc ttcggagtcg
     1681 atgacgttga gtgtcacggg gtgggtgtaa tgcaaacccg gagtcagtaa catgacttgg
     1741 aaatttgaat tactctcttt ccaaagactg tgttcaggat taaaccggat cccttgatat
     1801 gcttcgtagg atatttcccg cataattttg gggatgggtt gaggtgcttc atagggttta
     1861 tctgctaaag ctttcgctct ctgcaccact tgatcgaatg tgaaacccgc tgcaacatgc
     1921 agggggaaca tcaagaacat cgcgatcata ccggcaaaca ataatctcat cactgacgcc
     1981 cccgttaata aagattatga aactgcaatc gcccatatga aatatcccat tgagtccaga
     2041 cctgacaaag gtttgctcga tgggggaggt tattctgagt tggctaagag ggggatggtc
     2101 cacctgcctt atctgtgccc ggtcacccat tgtactacat gggtatatga aaaactgagg
     2161 ggatgagggg aatttattgg gagaaatccc aggggtaaga gaggtggatc tctttcttcc
     2221 attggatttg ggagacaggg gaaccatttg agacggtagc acgcaaaaat atggtcctgg
     2281 acagtgtgaa caattccttg aggcaattct cgtcttagga ctgaatgaga atatattctt
     2341 aatcaaggat aatttggact cgattatagc ataatcttat ccggcatcta cccttgtttt
     2401 tgttgttttt aaatggtttt ttaattagat cacacgattc tggaatatta tcgaatgata
     2461 actgcgattg aaagaggaac cggagtacta tccaggagtg ctcatttcat taaaatgaac
     2521 ccgggcaaac caggttgctc ccttcggtaa gcatggtgct ggccggcttt gctggttgcc
     2581 tatgggccgg atccgaggaa atgggctgca tgcttttagc gccgaaggtc tttacgaggt
     2641 aataagtata aattgtgtgc ccagtatgta ggcaattacc catttatctc aggtcagcgt
     2701 gcagttaggg agcgtttcga aaatattcaa catagaatac tccgagggcg ctcgagagat
     2761 gcagggggcc ttatttattt gaaagtgagg taaacaacgc aagggttgag cggatgtcga
     2821 tatcgggagt atccgtggtc caggagacgc gtatcgaaag gtttgaggga aaggagggat
     2881 gggtgagagt tttccaggca agggattgag tggtggaaag tgtgtgcatg gtctgttccg
     2941 tgcgttcgtc aagacagacg aatgataaaa tgccaggagc atgaggcccg tcccattcta
     3001 tcgtccgaat acatggattt ggtcggagac aggctttcca ctcttctcta aacccttcaa
     3061 gaatttgatt gtttgagtgt gtttgaacct tgagatgaca ggctttggca aatccggcga
     3121 tcaagccgat gttttgagtg cctaattcat atccagccca ataaggttgc gtttcctggt
     3181 gactgtcctt cccgattatc agcgcgccgg tccccattgg accggtgcac catttatggc
     3241 cagggaaaaa atatgcatag ggagaaatct cttggaagga aaccggaata tgtccgatgg
     3301 cttgtgcccc gtctacgatg agcggcgtgt ggtgtgactg agcgagatcc tggattgtgg
     3361 tgataggaaa aatacgccca tcaaaatgag agacgtgact gatgacgatg gcctgaatgg
     3421 gttgttcatc caatgtgcgt tcaagagcgg aaagaaaccc tgagggggca tccgggtcga
     3481 gtgcaatgac gcggacgcca cggtcactca gtttttttat ttcatcgaga atcgtggaat
     3541 tttcatgtgt ggtggtcagt aggacatttc cagatttcca attgattcga gaaaggacaa
     3601 gactgcacgc ggttgtggta ttcggcatga aggcgagccg ttgttcatcg ttcaacgtca
     3661 accaatcggc aatggtccgt cgacatcgtt gaagggtgtc ccggtaatat tgaatgccgg
     3721 catccgcata caacaagtgg ctgaacgtat cgagtgtcct ggaaatctct tgctgcacct
     3781 cctgatggaa cggagcgagt ccagccgggt tgagatagat catgggaata agttggcgga
     3841 gcaccctgcg ataatgaggt tagttcggat ctgagccatg atggagaagg tcctgccctt
     3901 cttgaatccg gcttagggtt tttttgcgag cgcgagacca atcaggctgt cagggtccga
     3961 gtaccatttg accaaggcaa aacccgaaga ggccagaagt tcttcggcca agggtcggtc
     4021 atatttcgtg ctgatttctg tgcggatttc ctccccttgc ttgaggtgga cttttaagtt
     4081 taagtcctga atgcccagcg tttgatcttc tcgcgaccgg agccacatct cgatgcgatg
     4141 gtcggcttca ttataccgcg cgacatggtc aaatgattct gaatcgctct gactaccgaa
     4201 ttgattatga agtactcgaa taatattctt gttgaatttt gcggtgatgc cctgctggtc
     4261 attatatgcg gcttccagcc gattacgatc cgtaataagt tggacaccca ataagaaata
     4321 atctcccggt gccatttctg aattcacaga ggagagaaac tcgtgagcgg cggtagcagg
     4381 aagattgcca atggttcctc caaggatgac cacgaggcgc ttgccgcctt ccggaatatg
     4441 ctccaaatgg accaagaaat ctcccaccac gccatggatg tggagtcctg ggtactcccg
     4501 gaccagctct tcggagaccc gtcggacaat ggcttcatcg acgtcaaagg ggacgaaata
     4561 gcgcagttgg ttgacgttgg ccatggcatc gaggaggact cgcgtttttg tggccgcccc
     4621 cgatcctaat tccacaagtt cttccgcttc gctgatgccg acgatttcat cagcccatcg
     4681 ggtgagcagt tgatgctcgg tgcgggtttg ataatattcc ggcaactcac atatttgctc
     4741 aaagagggtg gatccgcgtt catcatagaa gagtttggaa ggaagcgttt tgggatttgc
     4801 caataggccc cgggtaattt cggtttttag caaatccggg tcttctctgg ttaatggaag
     4861 atcgatggta attgatgggt ctgtttgcga tgtcatgagc tctccttggg gtgtgcaccg
     4921 tagttcatcc tctggcctcc actctcaggc aggagcgcag acggcgcgat gtttggaata
     4981 agatggaaat gaaaagttca aggaaaagaa catccttcga attaattttt ctagtgtaat
     5041 gcaagatggg ggaagatatc cgttcgaaaa attcgatgat cggccttcca taagcaaaac
     5101 tgaaaggccg tccgtggatg atggccaacg tatgaaaatt acatcagtag cttcactata
     5161 ccggcagagg ggtttccggg caagataagg gtattgacca aatcagatat gtaagcggct
     5221 cttttgtccg ccaaaacgtc acgttggttg gcgtgcatct tcaaatatca gatagcgatt
     5281 cggtgtggtt agtgaaccgg atccaggccg cgtggccgag cccattgttc tacccgacct
     5341 aagactccat caactgtcag agccaatacc gtggcgggta aggcaccgga taagatcaaa
     5401 ttgacatccg caagttggag ccctgccaca atggggacac ccaaacctcc tgcgccgata
     5461 aaggcggcca gtgtcgccgt cccgacggtc cagatggcag aggtgcgaat ccccgccatg
     5521 atggtcggcg cggcaagtgg aaggcgaatg gtacggagaa tttgccactc tgtcatccca
     5581 agggctcgcc cggtttccac aacatttggc gctgcgtccc gcaatcccga ataggtattc
     5641 cgaaggatgg ggaataatga atacatccat aaggccatga tggcgggaag cgctcccacg
     5701 ccaaatatcg gaatcataaa ggccagaagc gcgatggatg gaatggtttg caacatgccg
     5761 acaatgcgga taatcgtttc cgctcccttt cgccattgtt cgagggccaa tcccaatggg
     5821 atggccacca gaatcccaag gaataaacca aaaccggaga gcccaagatg ttctcccgtg
     5881 cgcatggcca gattagaacg cttggcccac atgtaggaaa aaatagaatc ggatgagtcc
     5941 gcactggcgt ctggttgtgt gtgattgccg gccacaagat tgagtgcccg taaagcgcta
     6001 tgcgcgactt ggggaaccgg ttctccctgt tcttgaatcc gaagatttaa ttcacgcatg
     6061 tgtggtagat ccaatgcatt ggtgagcaag ttgatgacct ttcccaattc cggatgacgg
     6121 ttgagaattt ctccgcgaac gagtgctgcg gcctcataag gcgggaaaaa gtgacgatca
     6181 tctttcagaa cgacaaagtc atatacggcc aagcgtccat ccgtggtgta gacatctaag
     6241 atgtctacct ctcccttatc tgtggcctga tattttaagg tttgttgcat gccgatcact
     6301 tcctgaaact gtaacccgta ggattgattc aaccctacca atccatcttc tcgctggata
     6361 aattcatacc caaatccggc tttcatttgt ggtgcgacat gcgtgagatc agagattgtt
     6421 cgcagtccta acgtttgggc ttgatcacgg cgaagggcaa gcgcataaga attatcaaat
     6481 cctagcggtg ccatccacca gatatcccaa cggtgaagga actccgtgcg aacatggttc
     6541 aaaacagata ctcgatcgtt cattgggggt tcaccgagaa tggtaacgag ccctgttccg
     6601 gtgtattcgg gatagaggtc aatccctccg gtttttaacg cttcaaaaca gacttgagtg
     6661 ccggcgagtc ccaggcgacg cgtgacggtt aattccgtac gggcctcaat gagttgagca
     6721 aacatttccg ccagtagacg gctttccata aagttctttg atcccaccac aatggtgtca
     6781 gaggcccaaa ccggaaggga aaaagaatag agcagtccca ggagtccgac cacgaatcct
     6841 atagagatcc tccctctgcg ttgtctgatc cgataggtgg tcatccctct cctactaacc
     6901 ccgatgggcg tgataatgct gaacgagcaa atgaacatag ggtgtggccg gagaatggag
     6961 aatttcgtgt ggagtcccca tttgctgaat gtgtccttct ttgagaatgg tgatccggtc
     7021 gcctaaccgg aaggcttctg acatgtcatg ggtcacaagg accattgttt ttcgcaactt
     7081 gatttgaaga gagagaaatt ggtcctgtag atcatggcgg gtgagcggat ccaacgctcc
     7141 aaacggttca tcgagcagga cgatgggcgg atcggctgcc agggcacgtg ccacggccac
     7201 gcgctgtcgc tgtcctcctg agagttcaat ggggtagcgt tcggcaattt gcgaaggatc
     7261 cagattcacg agagataaga gcatgtcgac tcgattgagt tgctgctccg atgtccaacc
     7321 taataagtga gggacgaggc agacgttttg tttgatagtc cagtgaggga agagcccgcc
     7381 atcttgtggg atatacccca ggcgtcgacg aagatgaatg ggatcctgta agcaggccgg
     7441 cttgccttga atgacgacgg taccgtcggt tggttctatc aggcgattca agagccgcaa
     7501 aaaggtggtt ttacctgacc cgctctcacc aatcagcgcc atggtttccc cctctttcac
     7561 atgccattca atattttgaa gcgcataggt gccatccggg aattgtttgc ttacctgttg
     7621 gatttccagt gcccacggca tcatatggac gagtttcctg gtgtggggtt tgacggagta
     7681 agaatggtgc tgtgatgcag atgcgtcagc gtcatgatga acgtgtgggg gttttcccat
     7741 ggccaggata acagggttca aggggagaga ctacccatgt tatcaacacc tgctgcaatg
     7801 ttggtgtagg gaatgccttt ataattttga gaacaagcag gaagaacggg atgcaagtgt
     7861 gccaattcgc tcatgctccc ctattcacct ccgcacggtt atttcatcaa tgtgccttca
     7921 tctactggct ctgaattcaa acgtggaatt gtatggggaa atttattggc ctttggctta
     7981 tttgggatag gcatagtggt tgcaccgtag caaaatttgt atggtgagaa aaaagacgga
     8041 agcctaacta cgggtcctca taaccgggtt ctcaatttat ttcttcctag gagattcgaa
     8101 tgaacgccca cctaattgga aactcttatc gtgttgaaag tcaggccttg acccatgaac
     8161 ctggaggctg ttgccgatgt ggtggattca tgtataggga gcctgttgtt gattcaggtg
     8221 aaaatgaaat ggaatttatg gcttctcgct gtctgcaatg tggagatgtc gtagatccgg
     8281 ttattctcag gaatcgatca gggaaacaag ttttgagtag tagcactgga agggctcggc
     8341 attgaatctt ctcatgcttt gtgcgtggtt aggattgaga aaggccacct gccagaaagc
     8401 ataatcccat gaaggtgaac accttgattg ggcacgatgt cgggagtatc gatttaaaga
     8461 caaaaaatct taatggtcgg cacccggagc ttgcgcctga ccttccggaa agcggtaaac
     8521 gcatactagt tttgggtgtt gaccttaaaa catggattgg gttgacagac agtctttggg
     8581 atttaggatt tacgcttaaa cgccttccct cttccttgca agaattggat caattgaagg
     8641 atgctttcgt cgatggtgta ttgtgggact ttgaatcgag ttccctgaag ggtttagctg
     8701 tcgtgtctca gcttcgcgga ggatcacctg tcctcccagt gatagtcatc gcgagctcct
     8761 caaacaaaga gctgctgata aaggctattg agaatggggc catggatttt attaagccca
     8821 tagatcacac ggaattccga aacaaatgtg tgcggttgtt tggatagcgg atgagatcga
     8881 taaagtaagg caatttcagg gaggaacctt catgataccg ttatgggttg ccgactccca
     8941 ggaactttct ccggaaaatc catctataag cgtacagtcg aaggagaaat ttgatgggaa
     9001 agatcctgaa tctccaaacg ctgtttctat tctggttgtc gacgacagtg ttgaatgtcg
     9061 catgatactc cgcatagcaa ttgaacgaga gggttatcat tgtgttgagg ccgcagatgg
     9121 agtggccgcg ctcaaggtat ttcaggaaaa aacgattgat ttcatcatca cggattttga
     9181 aatgccacat atgaatggat gcgaatttct tgaaatatta tccagttcgg ccatcagttg
     9241 tcctcctgca gtaatgatta cgggaaacct tgaggattct gtgcgaatca gagcgacgca
     9301 tgccggggca ttggccgtcc tcttaaagcc ttttgatctc aggaagtttc tggaaatggt
     9361 ccgggaagtc gtacatcgca aacaagaggc ccatcaataa gggcgtatcc atttcannnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatca agaattgaag cggaccttac
     9541 acgatctccg ggaacgggtg aaggaactga ccgctcttca ttccaccacc caattgtttc
     9601 acaacgaaga cttatcgact caggaattgc ttcagcatat tgctgatctc attccgcctt
     9661 cctggcaata ccctgaagtc acagccgccc gcattcgttt cggacatctg gaaattacga
     9721 cgcctaattt tgtgttatcc ccgtggaccc agcgggagga ttttatttgc cgggatggaa
     9781 cgacgggtga aattgaggtc gcgtatctgg aggaacggcc gacggaagtt gaaggcccct
     9841 tcctgcaaga ggagcgaaat ttattgcact cacttggaga gatggtccgt ctgcagttgg
     9901 atcggaagcg gacgcaagcc gctcttcgtg aggcgcatga tgtgcttgag cagcgggtgc
     9961 tggacagaac gtccgagctc acgacggcca atctgttact gtcgcgggaa gtggcgcaac
    10021 ggaaagaagc cgaagcgaaa ttggagaaat tgattgcgga gattcagaag aatcatgatg
    10081 atctcattgc tattttgaat caattgcata ttggaactgc cttgaccgac caggatggaa
    10141 aggtgacctt tctgagtcag gcggcacaac agttatgcgg aaaggcgaat ttccctcatt
    10201 caggtgagcc gtggcaagcg ttgtttgacg atccctccta tcatgctcaa atagaggata
    10261 tggtgaatcg gccgactgga gaacgggaga gaatcttagt gcgcatgccc gcggttggtg
    10321 gagaatgcac tcgttggatc aatgtggatg tgagggatga tccacgagat ccggaacgga
    10381 aaatgttttt cttgtacgat gcttcggagg tgcagaccct tcggcaacta ttgggagaga
    10441 cgggaaaata tcaggatctt attggcacca gccattccat gcaattggtt ttccagcaga
    10501 ttcaggattt ttctcgagtg gactccacca ttcttattga gggagaaacc ggaaccggaa
    10561 aggaactggt cgctcgtgcc attcaccaag ccagccaccg gaatgcgttt ccatttattg
    10621 ccgtgaattg tgcggggtta accgaatcac tggtcgccag ccaattattt gggcataagc
    10681 gtggggcttt tacaggagcg gtatcagatc aacagggttt gatcgaagcc gctgaaggcg
    10741 gaaccttgtt tctggatgaa attggcgata ttccgctcac cgtccaaacc agtctgcttc
    10801 gggttctaca agaatgggaa attacccgtc ttggggagtc ccggcttcgg aaagtggatg
    10861 ttcgtgtgct tgcggccaca catcataatc ttgccaatga tgtcactgcg gggactttcc
    10921 gggctgacct gttgtatcgg atcagggtcg gaagagtgtt gttgccggcg ttgcgggagc
    10981 gacgggagga tatcccatca ttaatcagtc attttctgaa tcaatatcgg gcgacaacag
    11041 gaagacctgt tgaggccgtg agtaatgaag ctctccggct gttgatggcc tatgcatggc
    11101 ctggaaacgt tcgagaactc aaacatgcca ttgaattcgc cgtcattcga tgtcctcaga
    11161 cgattattcg acctcaagat ttaccgcctg aagtccttaa tcaggatgaa agtcttcttg
    11221 taataccttc tgaagaaagg actccaaccc atgagaagga aaagattctc tctgcattga
    11281 aggaaacggg aggaaaccgt tcagctgccg ctaagctgct tgggatcgcc cgcctcactt
    11341 tgtatcgtcg aatgtcacgc ctcggaattt ctactcgccg ttaaattact tcctaattga
    11401 aagctttctt ctgaggggcc gttgtatgtt ctggctctat cccaggacca cttcgactta
    11461 aataacctga gcccagccag gatgagagcc caaaaaaagg agaagcccta gttagcgaga
    11521 tctttcaaca cttcttttgg gttcgtcacc tccatgaacg gatgatggtt tttcgctcca
    11581 catcatctgg tggagaagta tccttcggtt ccctgaaaca attccaggcg catgatgaat
    11641 gaaataataa ggtgtaactt aatgtaacta gctgtaacag aagtgcttca caaggatgtt
    11701 actttgtaac aaaaaaatga ttattgattt cattataagc atttgaaaat aaagaaaaat
    11761 taatgatttt ggcctgatgg catctaaatt ggattgaaag acaattgtgg gtgaaagcat
    11821 ttctagcaat taactggcat acgtttttca gtgttacggg tctggaagga ggaagttatg
    11881 aataaaaaca tattcattcc atttattctg atggggatgc tcctaggact tccgcaattt
    11941 gtcttggcta gtgagggcag tcaagaaggg aattcttcat ggaaactgga aatgcaagat
    12001 tggtcgattc aggatcacat ggctgcggca aaagataaag aggccgaagc ccagtcttta
    12061 cggagccgtg ttcgccattt gggggagcgc atagcccatt ttgagaaaaa gccacaattc
    12121 gatccaaagg ggatgcagag aaattccatg aaacttattg cctcgacttt gaccgaggaa
    12181 ttaaatcgtt taaataaaga gattgcctgg catttccggc aagcagacca tgccatgctt
    12241 atagagtaat agataatgcc tgtatgagta aggatatgaa tgaatttctt tcagcgtttc
    12301 caagaataga ggagggcatg aaagaggatc cctgtgcagg gatagagaga ggtatcctgt
    12361 ttcggatttg gtttttgtgc ctaacggata tgtcaggtat ttctgccaac ccgaggctgc
    12421 tcgaccgcta gggcatacaa tttttattga tactgttggg atcctggcat gaatgacggt
    12481 agttgtgggt tgatgaattt ttgacgagag tatgcgacgg cacaggttcg tcattgcgtc
    12541 taggaccatt tagaaaatcg acttgctgaa ctaaagcgta atgttagtct ccccctcttt
    12601 tgaacgaacc catttttttg gcggaagaag ccgccttcta tccagtagta aaaggtctat
    12661 cctcgtaaga gtatgtttgg cgaagccctg ctgcgtacgc acgggacggt caagctctta
    12721 atcagtgtgg tctaatgatt ttgtttgaag agcttgggtg agtcgccaga gcggacctca
    12781 aaaacctgac agaaacgtcc ttcccgaaag ccgatcaagg cttgaaaatt tccaggcatg
    12841 ctggtcttca tcttctatgc tcggttccgg ctttagttta ctaagtataa acactataaa
    12901 gtgccaataa gtaaggtatc tgagaagcca aaaagtgtaa ctatatgtaa cttactggaa
    12961 cacttttgct tcatttaagt gttgcatgtg ttacgaaata attaagtaaa ataaatacta
    13021 agttattgtt ttttaaacaa aattatgcag ttgccttgtt ggcatataaa ttggatattg
    13081 agatagtgtg atgaaaattt cgtcaatgct aactgggcag gtttgatgaa ctctggttca
    13141 ttaataataa aggagaggga tatgaataaa aaaatacttt ttccactttt ccttatgggg
    13201 atgctcttcg gccttccgca atttgtctgg gctgctgaga acagtcaaga acaaagttct
    13261 gcctggaaag ctgaaaccca agggtggtcg attcaggatc atatgactgc ggctaaagct
    13321 aaagaaggag aagtccagga tctacagagc cgcgttcagc aactggaagc aagaatagcc
    13381 cattttgaaa aaaagcccca ctttgacccg aaggggatgc agagaaattc catgaaactt
    13441 attgcctcga ctttgaaggg ggattctgaa attttgaatc agaggatcgc ctggcataat
    13501 aggcaatcgg atcaggcgag tctgcgggag tagtggtcta ctgaatgatt tagttgtaat
    13561 tgtcaaaaaa tgtctaatgg cgttctttgg aacggggagg ttactaagat ccccgttcct
    13621 taagaacgaa taagttctct cattgtgttg tccttgtgcc tgacctaagt gtcaggcatt
    13681 tttttttctg ctaagtattt tcgactgcca tgggttcgtt ttctatcacg ccatgatgac
    13741 tcgaaagtga ccaagttgct tcatttggcg aatgagatcg ggagagaacc tttccgcgat
    13801 acctaccaaa gcaatgccta agaaaagagt aatggataag aggccagagt aatagatcca
    13861 tacgatagat agatgtgcga ttaagcacgg tatcccagtg cattaccgct caatttcctg
    13921 gttcgaacat gaagtttttt taataaaatt tcctttataa gaacaagtca ttacagtagc
    13981 cgaatgatat aatgggcgat ccggtcatgt gagtcattaa gctattgaaa aataaaataa
    14041 atcggactta aactttacct cttgttgggc gaccattacg actaggtgaa taggtcttgg
    14101 tatgcaacaa ataaacggtt gatggcttgg taattgccgt ctcttttaga gaagaaatga
    14161 agctctataa attttcccca tgactgactt tttcttttca aaattattcg atattttagt
    14221 cttaaacacg ggttataaat tgtgatggtt ttcttggtat gagccgacag tatttcatga
    14281 attatgacca tgttcagaaa attttccgaa aggtcatgat ccttcgcaca tatacttgag
    14341 gttgcgtgaa gggattagtg atgaaatttg aaatgttcca ccaaaccgat caagttggcc
    14401 ggcattttta tcaaaccttg ctggaagtga cgaatgtctt aaattcccag cggaatactg
    14461 aagatctgtg gaaagccatt acgggacaca tcaaaaaggt gatctcatgg gaacgcgcgg
    14521 gtattacgtt gtatcaccat gatatagatg cctttcggtt ttacgcggtg gaaacgagta
    14581 tgcctgtggt caagttgaaa tgtgatgcca ttattcctcg agaaggaagt gccatgggat
    14641 gggtgtatgg tcaccggcgt cttcacatac ggccccattt acaacgaaac ccggtatttt
    14701 tagaggatgc gtattatcag gaagagggtt taggcagaat gatcaatatt cccctaattg
    14761 tcggggataa ttgttttggg tctctgaata tcggcagcgt ggaatcgggg gaacctgatc
    14821 cggaaaatgt cttgtttttg cgccaagtgg ccacccaaat tgcctttgcc gttgatcttg
    14881 tgcgtgccta tgaagagatc aaccatctga aagaacaact tactcgtgaa aatgcctacc
    14941 tcctggaaga aatcaaagtt agtcatgatt ttggagcgat ggtcggcaac agccagaaat
    15001 tcaagaacgt cctggacctg gcccaggctg ttgcacccac cacgacttca gtgctcatca
    15061 tgggtgaaac gggaacagga aaggaactgt tagcgcggtt gcttcatgag ttgagcccca
    15121 gaaaagataa accctttgtt cgagtcaatt gtgcggcgtt accttcaggg ctggtagaga
    15181 gcgaactttt cggccacgaa aaaggcgcgt ttaccggtgc agaaaatgga agacccggaa
    15241 aatttgaatt agcgcataat ggcactttgt tcctagatga aatcggggag atgccaattg
    15301 aagcacaggc caaactcctg agggttttac aagatgggat tgtggaacga gtgggcagta
    15361 tcgaggggaa ggatgtcgat gtgcgaatca tcgccgcgac caatagcgat ttaagcaagg
    15421 cgattgcgga ggggcgtttt cgttcagact tatattatcg attgcatgtc tttccgattg
    15481 tcgtgcctcc tctccgtgaa cgtctgcacg atattcccct gctggtgcga cattttatgg
    15541 aaaaaaatcg gttgcaattt cgacgaaatt gtcaggatat cgatgatcag tcaatggagt
    15601 tgctcattca gtatccatgg ccggggaatg tacgggaact caaaaacgtg attgaacgag
    15661 cgatgattct ttcccaatcg acgctcgtgc atgtggaaga atcattgttg ccctcaccgg
    15721 gacatggcat ggatgccctg ccgcccactc aattgaagga cgtggagcgc actcggattc
    15781 tacaagcctt aacggcttgt gaatggcgga tagatggccc attgggggcg gccaaacacc
    15841 tgggattgca tcccagtaca ctgagaagtc gattgaaaaa attcggaatc aaaagacaat
    15901 ctgaataata tattcaagcg gggactgtgt cgttgagatg aaaagccgga cgatctggag
    15961 tcaacgaatc aatatcgtcc gccctctagt tttgccccta ccaccttagt cccctctcgt
    16021 taagttgtag agtccagaaa atttcccatt ttcatcaatt gctgaatgaa gcaaagtgca
    16081 tcttcatgag aatttcaata gcccacaaat tcttcagagc gaatattggt ctttggaatg
    16141 cctgcagact ccagcatctt tcgtaatcca ttaaccattg cagggggccc tacaacataa
    16201 tacagagcct tttccgtttc tttgacatat tttttgagca tgacttggtc tatgcggccg
    16261 gtttcccctt cccagatttc ggtgcctctg ttcgggcttg tcattgttcc aatacatgta
    16321 aaatgctcat tttccttttg gagggtttgc agttcatcaa ggaatgccgc atctttcgga
    16381 tgccgattcg aatatatgag aatgattcga tgtgaaagtt tgtggtgggc tgattccacc
    16441 agcatgcttc ggaaaggtgt aatcccgatc cctcccgcga gaaagacaat cgttcgggaa
    16501 tcgtcggtgg gcagaatgaa ttgtccgaat gggccttcaa tatcaattga agccccaata
    16561 gacatatctg tcaatgtgcg tttaaaggcg gtgtcccgaa gacgtgtggc aaccatgaga
    16621 tgttgttcgg aaggagagct ggcaagggtc aaagctcgtg tgtggccctc tggatcggct
    16681 tttgaaagat tcggtaatga cagatcgata aactgacctg cggtaaatgt gaatccaggc
    16741 ggccggtcaa aataaaatgc cattgttcct gtagcgatgg cctgacgttt cttcaatgcc
    16801 acgtgatata aagaaggctt ggtgagtttt atcattgagg ttgctccgtg gtcattacga
    16861 atgagaagag gcgaggcagt gctgattcca tcctatcgtt tgtgtaaggg gttgagtcaa
    16921 atttggtatg tttaatagac aaacgggttt atgctccttc gaggactgag gggaaatctg
    16981 gttctatggt tgtatcggtt tcaagaaagg attgaaggac cgtggaacga attgagagaa
    17041 attaccgggc gctactgaat gtcgcgattg tactcaattc acaaagggat accagtagtc
    17101 tctggcaagc gattatcgag caaattgaac aggtcatgcc ttgggcacgg gcgagtgtga
    17161 ccttatatga tcgtaaatcg gacgggttcc aattttatgt tgtgacgact catttggcca
    17221 gagtcgtggt ccaaggtgat acggtgatac cgcgtgttgg gagtggaatg gggtgggtat
    17281 acgatcacaa aacgttacat gtccgtcctg acctcaacca ggaacaggtt tttctcgaag
    17341 accggtggta tgtcgaggaa ggacttggac gcatgattaa ccttccgctg ttggttagag
    17401 ggaagtgtgt gggggtcttg aatcttggaa gcgttgatgc gggtgacccg gaccccgatg
    17461 cgattgagtt cttatcacaa gtggccatgc aaattgccta tgcgattgat catgttcagg
    17521 cctatgagga aattaatctg ttgcgtcagc atttggcacg agaaaatgtc tatctggtcg
    17581 aggaactcaa actgacaaaa catttcggtg ccatggtcgg acggagtcag gtttttcagc
    17641 acgtattgga actcgctcgc gaggtggcgc ctacgacggc gacggtcctc atcaccgggg
    17701 aaacggggac cgggaaagaa ctccttgccc aagccattca tgattggagc ttgcgtgcta
    17761 aaaagccgtt tattagggtt aattgtgcgg cccttccggc aggattggtc gaaagcgaat
    17821 tattcggtca cgaaaaaggc gcatttaccg gagccgacac caggcgtgag gggcgttttg
    17881 agttggccca tggcgggacc ttgttgttag atgagatcgg ggaaattcct ttggagaccc
    17941 aagcgaaatt attacgagtg attcaggacg ggatggttga tcgtgtggga ggcaagcagt
    18001 ctatcccggt ggatattcgg ttgatggcct cgaccaatgc tgatctgcct tcagctattg
    18061 agcaaggtcg ttttcgagct gacctttttt atcgacttca tgtgtttccg attaccatcc
    18121 ctccacttcg tgcccggccg gaagatattc cagaactcgc acagcatttc cttgagcact
    18181 atgccattaa actcaagcgg ccctgcgaat tgattgaacc ggattctctc caacggctta
    18241 tccggtattc ctggccgggc aacattcgag aactggaaaa tgtcattgag cgggcggtga
    18301 ttctctccca tcaaaaggtt ctgcgtattg atgagcggct cttgattctc cacaacacaa
    18361 cgcaggaatc caaggccccg gctgggttag tcgattttga acggcaacat attctccaga
    18421 cgctggcttc gagcaattgg cagatcgagg gggagaacgg ggcagcggaa caactcggtt
    18481 tggctccgag cacactccgg agtcgaatca aaaagttagg tcttcagcgg ccgaatggct
    18541 caacccccag aaagtagtct ttgcaatatt cccttccccg tagagtccgt cttaactttt
    18601 aagatcttcc tgcatgatgt gtcgtatatg taccctacga tgctccaccc gactcatgat
    18661 gtcgttcatg tgatccaaaa tatagtttgt tcatttcaat gagcctgtcg ggccgagtga
    18721 tcctgctcga ataattaccc tggtctcttt ggcgaaggag atggaatgtt atcctgatgc
    18781 cggtgccttt tgtaaatttt taacccccac acggattaaa ggctcagatt gatgtagtct
    18841 atttcatgcg gcagtctgtt ctcccgtcat tgcccgccag cgattgacag gtcaggtatg
    18901 tagcggcccg gtggaacatc ttagattggc acccagacct attcggaaat tgtgttagcg
    18961 catcaggtct tctggatcca atgtgaagct tgccattcat ctcaagaatt tttttactcc
    19021 ttttctttat ctgattttct actttttcaa aatcccattc cgcagaaaag ccgcgtcata
    19081 tcaaggttta agtgctagag ccaccacgga ctgtcgtggt tatgtctatc catcttcatc
    19141 aaccatggac ttttttatct gaatcaaagc aaattttaag aatttattcc gtatgggttc
    19201 tgaaccggac catggcttag tctatgcata tcagcgtgaa gctaactcat tgagaacaaa
    19261 tgaagagagt ggttgtcatg agattcctga tcattacaga ttatgagggt aaaggggccc
    19321 gtcatgtttc cggcaatggt attcaaggcg aggagggttg ttcctgttca tgagtaatga
    19381 ccgttcatga aattttatct cgatacagtg agtctcacag aaattcggga gattggcagt
    19441 ctgggaattt tggatgggat agctgtgaac gccagtttga tttctgagca gggtttgaat
    19501 ttttatcaag gaattcggga aatctgtcgt tatgtggaca gcccaattag cgtgggtgtg
    19561 ttgagtattg aagaagaagc catcgtcaag gaaggcatag aattatcaaa aattcataga
    19621 aatgttctgg tgaaatgtcc tttgacacct gcaggtctca aggcgacgaa gcgattgacg
    19681 gccgaaggtc tccgggtaaa tgtgtctctg tgtttttctc ttacgcaagc attgattgcc
    19741 gcgaaagccg gtgcgtggtg tgtgtccatt tgtcccgatc acaccggtga caatcaatcg
    19801 atgggaaacg atatcactcg taaaattgtc acagtttttc gagactacgg gctttccact
    19861 caggtgcttc tcgcgggtat ccgttctcct catgaggtgc ttgaggcggc gttggcggga
    19921 gggcatatct
//
LOCUS       DAORAQ010000040        19777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_494_length_19777_cov_62.059122, whole genome shotgun sequence.
ACCESSION   DAORAQ010000040 DAORAQ010000000
VERSION     DAORAQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 19777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19777
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_494_length_19777_cov_62.059122"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKM72_05765"
     CDS             complement(<1..463)
                     /locus_tag="PKM72_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60326.1"
                     /translation="MVDKNFYIIRSDYGEIQIEVTPQTQLSESFEFGDRIKARVTPQD
                     KALSIVRATPDESPGIRLEEGLLPSIPTAPSIVIPPESESIPSKNDTSTPAPKTPPKF
                     RTVVAEVLMIDENMYIVRGDRGEIRIEVTPQTSISESFKFGDRIKAKILPND"
     gene            480..704
                     /locus_tag="PKM72_05770"
     CDS             480..704
                     /locus_tag="PKM72_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60327.1"
                     /translation="MIRTFGDTFVGGVVGSGLLVLEEGVDGGGGAVVPGTLLCVPIGS
                     PDWAFVMATALSFGKRIAFMRSPKVNDSVT"
     gene            complement(1009..1084)
                     /locus_tag="PKM72_05775"
     tRNA            complement(1009..1084)
                     /locus_tag="PKM72_05775"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1049..1051),aa:Phe,seq:gaa)
     gene            complement(1265..1654)
                     /locus_tag="PKM72_05780"
     CDS             complement(1265..1654)
                     /locus_tag="PKM72_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60328.1"
                     /translation="MLEAALNANEDVLSAGDLIPTDNTTIKKPIDVRKPQGWEKRLLG
                     ETYSQNTFQNNRFQSQNSSLLHLKSPGKLPGIELGVEMHSIKAFESIIPSENLPKGLD
                     PKQDKPLLLPPSISSPDYNGGFIRFTW"
     gene            2259..3569
                     /locus_tag="PKM72_05785"
     CDS             2259..3569
                     /locus_tag="PKM72_05785"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNP60329.1"
                     /translation="MPTDESQNDIEVSAEEEKISPEEYLKPLLQRAKQAARPLSGLIA
                     MKKNAALMAMADALEAGEEAILEANEKDLSAFEENSSQTAMADRLRLTQSRIADMALH
                     IREIAQLRDPVGESMGLWQRPNGMRVGRVRVPIGVIGIIYESRPNVTADAAALCLKSG
                     NVCVLRGGSEAHHSNLALATILGEAAQKAGIPEGAITFIDKTDREVVLALLKSHRFVD
                     LIIPRGGNTLMDTVTQHATIPVIKHDKGVCHIYVDSDVDLAMAQRVSFNAKVQRSSTC
                     NSMETLLVHEKVAKKILPPLADEYIEAGVEIRGCPKTCQILSQAKPAQDDDFGKEFLS
                     LIVAIKVVKDIESAMDHIHQYGSRHTDSILTNDYDRALRFLREVDSSTVMINASTRLN
                     DGYEFGLGAEIGISTTRIHARGPMGLEDLTCSKYVVYGSGQIRE"
     gene            3698..>5422
                     /locus_tag="PKM72_05790"
     CDS             3698..>5422
                     /locus_tag="PKM72_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60330.1"
                     /translation="MDHPQALHFSANQIYRQEWKSAGGQIIEQPTGHCVFYGKHGQRI
                     LLVDPDGNPLHECLWEQKPSGGFSLASARLRLDWGQWVGIKPEGLVNTISLDLSRRQG
                     WERITQDDLRQMAARSLNSDLAMVRFFYRDEDVVLHGDGQATIRQVKDAFYVLPNGSF
                     EEAKFMSCMSRMEWSRIDYLPVVELFLSLLPGTGSATFEFIRGLYDDQNINGALPLHY
                     SGIPVYPSEAAFRLFSLFFTPSVSSSQSPLEVFLDVERSHEVHWLPASAFPVRYMDEE
                     QHLCVTVRNQMVQKVTWWDDPSGLSFNRIPESGLPVSDNRGVEVTTEGLWLIDGRERK
                     KLTIRPSWQLSKLNHSVSCQPLESTWRDAFPMRKPILTPVEAFSSVLLYPQKSEMIGE
                     QESQPFVFDFLDDFLEEHQDLFRARSNATQVLLSLCEAGLGSCLQYQEFQIHTVWYKW
                     PHFAQKHAQSIWNRLSRMDRLAWFPNFQFLPFERHMLETLPTRYDWIYLWIPFSDYSN
                     HTMIDSWVKFLKTYLSEGSVGCVAGPPVLEGYLRSQGLQILHSAAGSTLPPFRIHQTI
                     LPHGWLNPE"
     assembly_gap    5423..5522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5539..6090
                     /gene="pyrR"
                     /locus_tag="PKM72_05795"
     CDS             5539..6090
                     /gene="pyrR"
                     /locus_tag="PKM72_05795"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNP60331.1"
                     /translation="MNTSPRPHERLLMDGQEMSRTLTRISHEILERNKGIEGLALVGI
                     RTGGVFLAQRLAARIQQIEKRDVPLGELDITLYRDDLSIRKDQPEIRKTTIPFHISNL
                     KIVLVDDVLFTGRTIRAALDGLMDLGRPAEIQLAVLVDRGHRQLPIRANYVGKSIPTA
                     REENVQVFFEELGQDDRVSILTP"
     gene            6093..7034
                     /locus_tag="PKM72_05800"
     CDS             6093..7034
                     /locus_tag="PKM72_05800"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNP60332.1"
                     /translation="MGLERKDLLTIAALSAEQIQLILTTAESLKEVGGRDIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSTSSMVKGETLVDTARNIEAMQA
                     DIIVLRHPSPGAAENLARSVKSSVINAGDGWHEHPTQALLDVFTIKEKKGRISGLLVV
                     IVGDIAHSRVARSNILALTKLGAEVRVVAPPTMIPIGLEHFGVTVFYNLDEALIGADV
                     IIMLRLQRERLGQALLPSLREYAKLFCLTPERLKLAKQDAIVMHPGPLNRGVEISPSV
                     ADSKVAVILDQVTNGVSVRMALMYLLSGSGERSTLNE"
     gene            7044..8366
                     /locus_tag="PKM72_05805"
     CDS             7044..8366
                     /locus_tag="PKM72_05805"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNP60333.1"
                     /translation="MTKKTGQHPTTSHLLCIQGGIVIDPGHVNGRADVLIQDGNILEV
                     GFPLTNPLSQDSSITIFEANGWIVAPGLVDLHCHLREPGFEYKETISTGAASAAAGGF
                     TTICCMPNTQPVNDNAAITKFMLAQGQKAGKARVFPIGAITKKSEGEELADIGELVEA
                     GCVAISDDGRPVMNSLVMRRALEYAKAFGIPVVDHCEDLHLTDGGCMNEGMVSTELGM
                     PGIPDASEEVMVARNLALADLTGVHVHLAHLSTARSVELVRHAKGQGLPVSAEVCPHH
                     FSITEEAVRCYNSNAKMNPPLRTDEDVQALKQGLVDGTIDVIATDHAPHALQEKQLEF
                     DTAPFGIVGFETALPLTLRLVHEGVLSLEQALEKLTRSPAQLFHLPVGSIRPGDYADI
                     VVFDPQEEWVVDPNKFLSKSQNTPFGGWKVKGKVKMTLVDGKIVYDAR"
     gene            8370..8906
                     /locus_tag="PKM72_05810"
     CDS             8370..8906
                     /locus_tag="PKM72_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNP60334.1"
                     /translation="MRMKRINWGVVGFLFELCALILWVGGLVVIIGLVIPAVFNSFGM
                     EPAGHFLRRVFDGFVVMNVWILILLSVVAVIRFRAFGKNPSEKFAVPSVEWWLLAGMA
                     LMTFSILVVLGPQAMALQEEAFAAVSKEDKDIAYAKFFRLHMIVRGCHLVNFGLAASL
                     FIVKVRKILFFHPMAARS"
     gene            8955..10139
                     /gene="carA"
                     /locus_tag="PKM72_05815"
     CDS             8955..10139
                     /gene="carA"
                     /locus_tag="PKM72_05815"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNP60335.1"
                     /translation="MKPAVLALADGTIFRGRALGFEGETLGEVVFNTAMTGYQEILTD
                     PSYKGQIVLMTSTQIGNYGVTPEDNESHRVWAEGFIVREAASCRSNWRSRQSFEEYLI
                     EHRIVAIQGIDTRALTCHVRDHGSQMGVISHLSFDDDALREKAKAVPSLIGRDLVREV
                     TCSEAYEWTERSIVMDSPRGKSPIVPQDIAQTPLKLVVMDFGVKQNILRSLVDLGCQV
                     RVVPASTTAEEIRRLHPDGIVLSNGPGDPEGVPYAVETVKQLLGWKPLLGICLGHQVL
                     GLSLGLHTHKLTFGHHGANHPVMDLRTRKIEITSQNHNFAVGLPHKEDGCTTLKSQQF
                     DSAVGRMEITHRSVNDGCCEGMAGIESPILSIQYHPEASPGPHDSSYVFGQFLEMMKG
                     RG"
     gene            10145..11101
                     /gene="sppA"
                     /locus_tag="PKM72_05820"
     CDS             10145..11101
                     /gene="sppA"
                     /locus_tag="PKM72_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNP60336.1"
                     /translation="MTRGFLILILFLVSGCIHIDLLPGGGKLHEATLSGEGEDKVLLI
                     DISGMLTTGKSSGILEEPSLPARIKEELTKAEKDEHVKAIVLRINTPGGSVTASDLVY
                     HELKVFKKKRSVPVIASIMDLGTSGGYYIAMAADHIFAHPSTITGSIGVIMVTMNAEG
                     LLEKVGVQPAAIVSGPKKAMGSPFRPMNDEERAIFQGAIDHLYEQFLAVVEEGRPGLS
                     KEHIRTLADGRIFTADIARDQGLVDDIGYLDEALDLAKKEAKLEAAKVVTYTRGGGTQ
                     QNIYSQFDPPQIEPIRFPQVDTQSLFKVLTGGTPQMLYMWMP"
     gene            11138..12034
                     /locus_tag="PKM72_05825"
     CDS             11138..12034
                     /locus_tag="PKM72_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNP60337.1"
                     /translation="MTNDPPVSGNRFQCLVASTVCALLLLSVACQKAPGTARDQLIYI
                     SEEKEIALGLAAFREVLKSAPLSRDPEVTLMVNRVGQRIAKGANKPEYVWEFAVIEDD
                     DQVNAFALPGGKVAVFTGILKYTKTEAGLATVMAHEVAHALQRHGAERYSRGILEQIG
                     QLGALAGAAAGGVDPGLAIGAMSAYGVGVALPNSREQETEADFIGLQLMAKAGYDPRE
                     AVPFWERMSGCPRKMIGKFCFRTQNAVPEFLSTHPSDVTRINRIEAWIPQAMKFYHPE
                     GLPPGVPESPLKKPTSESDLKS"
     gene            12044..15346
                     /gene="carB"
                     /locus_tag="PKM72_05830"
     CDS             12044..15346
                     /gene="carB"
                     /locus_tag="PKM72_05830"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNP60338.1"
                     /translation="MPRRNDIHRILLIGSGPIVIGQGCEFDYSGTQACRALKEEGFEV
                     ILVNSNPATIMTDPDLADRTYVEPITAEVVEAILEREHPDALLPTMGGQTALNVTMEL
                     VKRGILEKHGVQLIGASYESIQKAEDRELFKQAMARIGLNVAASGSFRSREEALQLLQ
                     TIGFPAIVRPSFTLGGAGGNIAYNREEFDKYIDWALVTSPVGQALLEQSLLGWKEYEL
                     EVMRDSKDNVVIVCPIENFDPMGVHTGDSITVAPAMTLSDKEYQHMRDAAIRIIREIG
                     VDTGGSNIQFSLNPDNGALMVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     IPNDITQVTKACFEPTIDYVVVKIPRFTFEKFDGVDRVLTTHMKSVGEAMALGGTFKE
                     ALQKAIRSLETDRYGLMSLHGLDGKKDTAGPDHPLHDRLQSQVRIPTADRLWFLADGF
                     RIGMSVDELYQLSRVDRWFLHEIEELVEFEAQWVRKSKEEFKTDRSHDNPKGCPDTLL
                     ILLEQAKGLGFSDQRLGQLIGREEDTIRQWRKSRGSGYGTVFKRVDTCGAEFEAHTPY
                     LYSTSGLTCESRPSQKRKIMILGGGPNRIGQGIEFDYCCVHAAMALKEEGIETIMVNC
                     NPETVSTDYDISDRLYFEPLTKEEVLRIILAERPDGVVVQFGGQTPLKLAVPLEQEGV
                     RILGTQPDAIDRAEDRKRFSDLLIELNLLQPQNGTARSSHEAHIIAVNIGYPVMVRPS
                     YVLGGRAMQIVYDADALDEYLQRHDMDLGRHPLLIDDYLEDAIEVDVDAIADGTDVVV
                     AAIMEHVEMAGIHSGDSACSLPAHSLGADILETINRQTILLAKELQVTGLMNIQYAVK
                     GGAVYILEVNPRASRTVPFVSKVIGVPLAKHAMKVMAGRTLKDLGFTQSPLFSHIAIK
                     EAVFPFTKLGVSDVLLGPEMRSTGEVMGIDRSFGWAFAKSQAASGMPLPLAGTALLSV
                     KDGDKPATLAIAQRLSQLGFSLNATKGTAAFLRKHGITVVTVNKLNAARPHIEDLLKN
                     KELSLVVNTVGGASSQDDSAPIRKAAVFGGIPYFTTIQAAQAAISGIEAMKKTSIEVR
                     CLQEYHSGLPAG"
     gene            15423..15899
                     /gene="greA"
                     /locus_tag="PKM72_05835"
     CDS             15423..15899
                     /gene="greA"
                     /locus_tag="PKM72_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNP60339.1"
                     /translation="MKIPITKKGYEALQAELARLRREDRPKNIQAITEAREHGDLKEN
                     AEYKAAKEQQQFIDTRMAELEHKLSMAQVVEIASGQSDTVVFGVTVSIVSLESQEEKR
                     YTLVGQEEADIKNGSISVQSPIGRALIGHRVGDIVEVHRPAGVIEYQIQSICFEEL"
     gene            15902..16702
                     /locus_tag="PKM72_05840"
     CDS             15902..16702
                     /locus_tag="PKM72_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNP60340.1"
                     /translation="MTSPISLVTLVTDFGDIDYFVPSMKGVMLGINSQIRIVDLTHRI
                     PAHGIEQAAFFLKSCYQYFPDGTVHVVVVDPAVGSSRRAILASTSRHFFLAPDNGVLS
                     YVLQEETSVEVRSIENKQYRLDSAGGTFDGRDLFAPSAAWLTKGQVPGSYGRLIHDYV
                     TLPLDPPRMQQQALHGRIVYVDHFGNAMTNITLADIETFRSVTKKEYSGLKIGDALIN
                     GIKTHYGEGAMGIPEALINSNGHVEIFVKQGRAVDRCELHVGQTVELI"
     gene            complement(16699..18078)
                     /gene="bioA"
                     /locus_tag="PKM72_05845"
     CDS             complement(16699..18078)
                     /gene="bioA"
                     /locus_tag="PKM72_05845"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNP60341.1"
                     /translation="MSHTALEKDDCEYIWHPFTQMQEWEGQTPLIIRRGKGSYLYDMD
                     GNAYLDATASIWVNVHGHQHPRINQAIRRQLTQVAHTTLLGLSNPPAIQLAKALIRLA
                     PKGLRKVFYSDNGSTAVEVGAKMAIQYWQQCPTPQPQKTRFIHLGLSYHGDTVGSVSL
                     SGVELFRRPFSSLLFPSHAIEPPHCYRCPLHMEFPQCQLACLDPLEKLLHTSHQEIAG
                     LILEPMVQGVAGIIPSPPGYLKRVRDLCTRYNVLFIADEVATGFGRTGLMFACEHEEI
                     TPDIMAIAKGLTGGYLPLAATLTTAAIYDAFLGEGHEGKTFFHGHSYAGNPLGCAAAL
                     ANLSIFKSERTLAKLQRRIPKLRKALESLSDDPWVGDIRQCGYMVGIELVQDRANKKP
                     FPADERIGQKIAMTARTLGMLIRPIGTIMILMPPLSASVKELEQMVSILKLAISVVRS
                     SRPSFSVQT"
     gene            18175..>19777
                     /gene="lepA"
                     /locus_tag="PKM72_05850"
     CDS             18175..>19777
                     /gene="lepA"
                     /locus_tag="PKM72_05850"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNP60342.1"
                     /translation="MSKAFPQTHIRNFSIIAHIDHGKSTLADRFLEITGAVSPREARA
                     QYLDAMDLERERGITIKAHAVTTRFKSSDGQDYQFNLIDTPGHVDFAYEVSRSLAACE
                     GALLLIDATQGVEAQTIANAYLAISNNLVIIPVINKIDLPSADVEGVKVQIRDVLGLS
                     SEEAFLVSAKDGRGVKEVLEGVVKVIPAPIGSIDQPLKALIFDSWFDNYQGAVVLLRV
                     MDGEVTPNMMIKLMFSGREFEVLEVGVFSPKRTKVSRLGPGEVGYICAGMKELSDTKI
                     GDTITDSKRPTSVALPGYRDVKPVVFCGLYTTDNAKFEDLRDSLEKLQLNDSSFVYEP
                     ETSLALGFGFRCGFLGLLHMEIIRERLEREYGLDLISTAPTVVYRVLTTKGETLVIDN
                     PSKLPDPSQIERIEEPYIKATMITPERYIGNVLQLCQERRGIQIGLQYLDPTRSMLTY
                     EIPLNEIVLDFYDRLKSRTQGYASLDYELLGYRESDLVKLDLLLNGETVDALSIIAHK
                     DKVQSRGRQLAEKMKELIPKQMFEIV"
BASE COUNT         4951 a         4399 c         5141 g         5186 t
ORIGIN      
        1 tatcatttgg taaaattttg gctttaatcc tatctccaaa tttaaaggat tccgaaatgg
       61 atgtttgggg agtaacctcg atacgaattt ctccacggtc gcctcggact atatacatgt
      121 tttcatcaat cattaaaacc tcggccacaa ccgtacgaaa ttttggaggc gtctttggcg
      181 caggtgttga tgtgtcattt ttggagggaa ttgattctga ttctggagga atcacgatgg
      241 aaggtgccgt cggaattgag ggtaaaagcc cttcttccaa ccgaatgccg ggggattcat
      301 ctggagtcgc acgaacaatg gataatgctt tatcctgtgg ggtaactctt gctttgattc
      361 gatccccaaa ctcgaaggat tctgataact gggtttgggg tgtcacttca atttgaatct
      421 ctccatagtc gcttctgatg atataaaaat ttttatccac cattaggatg tcggcaataa
      481 tgatccgcac attcggtgac acatttgtag ggggagttgt cggttctggc ttattagtgc
      541 ttgaagaggg agtagatgga ggagggggag ccgtggtgcc gggaactcta ctttgtgtcc
      601 caatcggttc gcctgattgg gcttttgtta tggcaactgc cttgtcgttt ggtaagagaa
      661 ttgcttttat gcgatcgcca aaagtgaatg actccgtcac ttgagtttcc gtggtgactt
      721 ctattcgaat ttctccacgt tccccacgaa ccacataaaa cgcgccatcg atcatcaaga
      781 agtccgcaac aacagtcctg gtttttggga gagggtaagg ttgagaagag gcttgggcca
      841 attgtttacc attaccttca gcggcaaatg gggcttgagg ccctagcatt cccagggaga
      901 atagaagaat caacaacgac ccagaaacat tgagaggaga tctaattgga aggagggtta
      961 tgagcttcat gtgagtaggc gagggaacag gggaagtttt tgttttcgtg gtgccgaggc
     1021 gcagaatcga actgcggaca ccagcctttt cagggctgtg ctctaccaac tgagctacct
     1081 cggcacattc atagaacctt taacttcgac ctgtttttat accataaagg attcttcctt
     1141 ttccaatcag ttccgcaact ttttcctgtt ttggagaatt cctaggagct gattttttta
     1201 ggccattctg actttgcttt cgggttattc ggagacttat gttgatgagg tgaagcaatt
     1261 ttcactacca ggtgaaccgg atgaatcctc cattgtaatc cggactactg atggagggag
     1321 gaagaagaag aggtttatcc tgcttgggat ccagaccttt tggtaaattc tctgagggga
     1381 taatggattc aaaagcctta atgctatgca tttcgactcc taattcgatg cctggcagtt
     1441 ttcctgggga tttcaggtga agcagagagc tattttgcga ttgaaatcgg ttattttgaa
     1501 aagtattttg agaatatgtt tctcccaata accttttctc ccaaccttgt ggctttctta
     1561 cgtcaatagg cttttttatg gtcgtattgt ccgttggtat cagatcccca gcggacaaca
     1621 cgtcttcatt ggcattcaga gctgcctcaa gcatcgaact ttccaatggg aagagaaaaa
     1681 gacataaact gaacgaaatg aatgtgagtg ccgggaacga aagatgggct acttttcctt
     1741 ttaccatgat gacatccctt cccctttcgg agtcaaaaga tattttatca tagatatggg
     1801 ttgtatgtat tgatctacca tctgcctacg ggtttcaagt agatgctcat atcctgcacc
     1861 attcattgtg ctttagctgt aggtaggcat ttcaggaaac cgtttcttgt ctacttatat
     1921 cgggactggg ataggctcgg gactgatttc gaaaaatgag cgttttccgg ttttcctgcc
     1981 taaaagagcg atgacacttg atagcaactt cggcaactgg cttcagaagt gatgaggtag
     2041 gtgaacagtt gaatgtatga gctgttcaaa gccaactcta tgatctatga ttgcataaat
     2101 tttaatgttg attcaggacg aaacatttga acatccactc caaacctcgc ttccttgacg
     2161 gtttttggaa ctgccaggta ttctcaaccc tgttccgctc ataatattgt cacctcgttt
     2221 atccgccaca ggcttctacc atggaaaaac ataagactat gccaacagat gaatctcaaa
     2281 atgatattga agtttcagcg gaagaagaaa aaatttcccc agaggaatac ctgaaaccct
     2341 tacttcaacg agccaaacag gctgcccgcc cattgagtgg tttgattgcc atgaaaaaga
     2401 atgcagcgtt aatggccatg gctgatgcct tagaagctgg ggaagaagcg attcttgagg
     2461 ccaacgaaaa agacctttca gcattcgaag aaaattcctc acaaactgcc atggcggatc
     2521 ggcttcgctt gacacaatcc agaattgccg atatggcatt acacattcgc gagattgcgc
     2581 aattgcggga tccggttggc gaatcgatgg ggctctggca acgacccaat ggaatgagag
     2641 tcggtcgagt gcgtgtgccg attggggtga tcgggattat ctatgaatct cgtcccaatg
     2701 tgaccgctga tgcggctgct ctctgtctca aatccggcaa tgtatgtgtg ctacgtgggg
     2761 ggtccgaggc acaccattcc aatttggctc ttgcgaccat tctgggtgag gcggcccaaa
     2821 aggcaggaat tccagaagga gcgattacct ttatcgacaa gactgaccgt gaggttgttc
     2881 ttgctctact caaaagccac cggtttgttg atctcatcat tcctcgcggt gggaatacgc
     2941 taatggacac ggtcactcag catgccacca tcccggtgat taagcacgac aaaggggttt
     3001 gccatattta tgtagattcg gatgtggatc tggccatggc ccaacgagtc agttttaatg
     3061 cgaaagttca acgttcctcc acctgtaaca gcatggaaac acttttggtt catgagaaag
     3121 tggctaagaa aatcttgcca ccgttggccg atgagtatat tgaggcaggg gtcgaaatac
     3181 gtggatgtcc gaaaacctgt cagatccttt ctcaggccaa gcccgcccag gatgacgatt
     3241 tcgggaaaga gtttttatca ttaattgtgg cgatcaaagt ggtgaaggac atagagagcg
     3301 ccatggatca catacatcaa tacggttccc gtcatacgga ctcaattctg actaacgact
     3361 atgatcgggc tttacgattt ttgcgagagg tggattccag taccgtgatg atcaatgcct
     3421 cgacgcgctt gaacgatggc tatgagtttg ggttaggggc cgaaattggg attagcacaa
     3481 cccgtatcca tgctcgtggt cccatggggt tagaggattt aacctgttcc aaatacgttg
     3541 tttacggttc cggtcaaatc cgggagtaag agcccgatcg gttcgatgcc gtggtcccaa
     3601 caatttctct cacaatgatc ccgttttgga tctttgttct cagagacgat ctgccttttg
     3661 acatttctca ttcctcatca tgaaccttca aagcatattg gatcatccac aagccctaca
     3721 tttttctgcc aatcagatct atagacagga atggaagtcg gctggtggac agatcataga
     3781 gcaacccact ggtcattgtg tgttctatgg caagcatggg caacggattc ttctggtaga
     3841 tccagatgga aatcccctcc atgaatgttt gtgggaacaa aagccatcgg gtgggttctc
     3901 cctggcatct gcccgtctac gattggattg gggacaatgg gtcgggatta aaccggaagg
     3961 attggtcaat accatttccc tggatttatc tcggaggcaa ggttgggaac ggatcacgca
     4021 ggacgacctg cgtcagatgg cagcccgatc cttgaattcc gacctggcga tggtgcgttt
     4081 tttttatcgt gatgaggatg tcgtccttca tggtgacggg caagccacga ttcgccaggt
     4141 taaagacgcc ttttatgtct tgccaaatgg atcgtttgaa gaagcaaagt tcatgtcatg
     4201 tatgagccgg atggaatgga gtcgaataga ttacctgcca gtggtggaat tgtttttatc
     4261 cctgttgccg ggaaccggta gtgcgacctt cgaattcatc cgaggtctct acgacgatca
     4321 aaacatcaac ggggcacttc ccttgcacta cagcggtatt ccggtatatc cctccgaagc
     4381 agcgttcaga ctcttcagcc tcttttttac cccttccgtt tcttccagcc aatccccgct
     4441 ggaagtgttt ttggatgttg aacggtcgca tgaagtccac tggcttccag cgtctgcctt
     4501 cccagttcgg tatatggatg aggaacagca tctttgcgtc acggtccgaa accagatggt
     4561 tcaaaaagtc acatggtggg atgatccctc tggcctatcg tttaaccgaa tacccgaatc
     4621 aggtcttccg gtttccgaca atcggggagt ggaagtcacg accgagggtt tatggttgat
     4681 tgacggccgc gaacgaaaga aattgaccat tagaccatcc tggcagctat ccaagcttaa
     4741 ccattcggtt tcttgtcaac cgctcgagag cacctggcgg gacgccttcc ccatgaggaa
     4801 gccgatactt actcccgtgg aagccttctc ctctgtcctc ctctatccac agaagtctga
     4861 aatgattggc gaacaagaaa gccaaccttt tgtttttgat tttctagatg attttcttga
     4921 agaacaccaa gacctctttc gcgccagatc taacgccaca caggtcttgc tttcactttg
     4981 cgaagcagga ttgggttcgt gtcttcaata ccaggaattt caaattcata cggtttggta
     5041 caagtggcca cattttgcgc aaaaacatgc ccaatctatt tggaataggc tttccaggat
     5101 ggatcggttg gcgtggttcc caaattttca atttttaccc tttgagcggc acatgttgga
     5161 aacattgccg accagatatg attggattta tctctggatt cccttttcag attattccaa
     5221 tcacacgatg atcgactctt gggtgaagtt tttaaagact tatctttctg agggtagtgt
     5281 cgggtgcgtg gccggtcccc ctgttctgga agggtacctc cggagccagg ggcttcagat
     5341 tctccattcc gccgcaggat ctactttgcc tcccttccgg attcaccaaa cgattcttcc
     5401 ccatggatgg ttgaatcctg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnatagcggt ccattaagat gaacacttct ccacgacccc acgaacgtct cttaatggat
     5581 ggtcaggaaa tgtccagaac cttaacgcgc attagtcatg agattcttga gcggaacaaa
     5641 ggtattgaag ggttagcttt ggtggggatc cgcacaggcg gagtatttct cgcccaaaga
     5701 cttgcggcca gaatccagca aatcgaaaaa cgggacgtgc ctcttggaga gttggatatc
     5761 acgttgtacc gtgacgatct ctccattcga aaagatcagc cggaaatacg aaaaacgaca
     5821 attcccttcc atatatccaa tttgaaaatt gtgctggtcg atgacgtgct gtttacggga
     5881 cgaactattc gggccgcctt agacgggttg atggatttgg ggcggcctgc agaaattcaa
     5941 ttggctgtgc ttgtcgatcg tgggcatcga cagcttccca ttcgtgcgaa ttatgtcggg
     6001 aaaagcattc cgactgccag ggaagaaaac gtccaggtct tttttgagga attaggacag
     6061 gacgatcggg tatccatttt aacaccgtaa ttatgggttt agaacgcaaa gatttattaa
     6121 ctattgcggc cttgtcagcc gagcagattc aactgattct gacgacggcc gaatccctga
     6181 aagaagttgg ggggagggat attaaaaaag ttcccgcttt gcgtgggaaa acggtggtga
     6241 atttgttttt tgagcccagt acccgtacgc gaacgtcttt tgaattggct gctaaacgtt
     6301 tgagtgccga tgtcattaat ttttcgcctt ctaccagcag tatggtgaaa ggcgaaacct
     6361 tagtcgatac cgccagaaat attgaagcca tgcaggcaga tattattgtg ctgcgccatc
     6421 catctcctgg tgccgcagaa aaccttgcgc gttcggtcaa atcatcggtg attaatgcgg
     6481 gagatgggtg gcatgaacac cccactcagg cacttttgga tgtctttacc ataaaagaaa
     6541 aaaaaggtcg catctcgggg ctcctggtag taattgttgg cgatattgcg catagccgcg
     6601 tggctcgctc caatattctt gctctgacca aactcggggc ggaggtgaga gtcgtggcac
     6661 caccaaccat gatccccatc ggcctcgaac atttcggtgt gaccgtcttt tacaacctag
     6721 atgaggcatt aatcggagcg gatgtgatta tcatgctacg cttgcaacgt gagcggttgg
     6781 ggcaggcgtt acttcccagc cttcgggaat atgccaaact gttttgtttg accccagaaa
     6841 gactcaagct cgccaaacag gatgccattg tgatgcatcc cggtcctctg aaccgtggcg
     6901 tggaaatttc gccttccgtc gctgatagca aggtcgcggt aattctggat caagtgacca
     6961 atggggtgtc tgtgcgaatg gcccttatgt atttattgtc aggatctggt gaacgctcaa
     7021 cccttaacga atgatcggac aacatgacca aaaagaccgg acaacatccc accacttctc
     7081 atctcctgtg tattcaaggg ggaatcgtga ttgaccctgg ccacgtgaat ggacgtgcag
     7141 atgtactcat tcaagatgga aacattcttg aagtcggttt tccactgacg aaccccctta
     7201 gtcaggattc atcgatcacc atttttgagg ccaatggatg gattgtggct cctggccttg
     7261 tcgatctgca ttgccactta cgggaacccg gctttgaata caaggaaacc atctcgacag
     7321 gagcagcctc agctgcggcc ggagggttta cgactatttg ttgtatgccg aatacgcagc
     7381 ccgtgaatga taacgcggcc attaccaaat ttatgttggc ccaaggacaa aaggccggta
     7441 aagcacgagt gttcccaatc ggtgcaatca ccaaaaaatc cgaaggagaa gaactcgcgg
     7501 atattggtga gttggtggag gccggctgtg tagccatttc ggatgatgga cggcccgtga
     7561 tgaatagttt ggtcatgcga cgagcgctgg agtatgccaa ggcttttgga attcctgttg
     7621 tggaccattg cgaagacctg cacttaaccg atggcggatg tatgaacgag ggcatggtgt
     7681 cgaccgaact cgggatgcct gggattcccg atgcctcaga ggaggtgatg gtggctcgga
     7741 acctggctct ggcagatctt acgggagttc atgtgcattt ggcccatttg agcaccgccc
     7801 gttcggtgga actggtccgt catgccaaag gacaagggtt accggtgagc gcagaagtct
     7861 gtcctcatca cttttccatt actgaagaag ccgttcgctg ttataattcc aatgctaaaa
     7921 tgaatccacc actgcgaacg gatgaagacg ttcaagcgct taagcaagga ttagttgatg
     7981 gcaccattga tgtcattgcc accgatcatg ccccccatgc tcttcaagaa aaacaattgg
     8041 aatttgatac ggcgccgttt gggatcgtgg gatttgagac cgctcttccc ttaaccctcc
     8101 gcttagtgca tgagggggta ttatcacttg agcaggcact tgagaagttg accagatctc
     8161 ctgctcagct ctttcatctg cctgttggaa gcattcgacc aggtgactat gcggatatcg
     8221 tggtgtttga tcctcaggaa gagtgggtgg tggatcccaa caaattttta tcaaaaagtc
     8281 agaatacgcc atttgggggt tggaaagtga agggaaaagt gaagatgaca ttggttgatg
     8341 gcaagattgt ctatgatgcc cgttaattca tgcgtatgaa gcggatcaat tggggggtag
     8401 tgggttttct ttttgaacta tgcgccttaa tcttatgggt tggcggtttg gttgtcataa
     8461 ttgggttggt gattccggct gtatttaatt ccttcgggat ggaaccggct ggacattttt
     8521 tacggagagt ttttgatggt tttgtcgtga tgaatgtttg gattttgatt ttgttgagtg
     8581 tggtggccgt gattcgattc cgggcatttg gcaaaaatcc ctccgagaag tttgccgttc
     8641 cttctgtgga atggtggctt ctggctggaa tggctctcat gacgtttagc attcttgtgg
     8701 tccttggtcc ccaagccatg gctttacagg aagaggcctt tgcggcggtc tcgaaagagg
     8761 ataaagatat cgcctatgcg aaattttttc gccttcatat gattgtgcga ggttgtcact
     8821 tggtgaattt tggtctggcg gcctcactgt ttattgtcaa ggttcggaaa attctctttt
     8881 tccaccccat ggctgctcga tcataattac tcttggtcgt acgacggaaa tgagaagatg
     8941 ataaaggaaa gaccatgaag cccgcagttt tagcgttagc cgatggaacc atattccgag
     9001 gacgtgccct cggattcgaa ggcgaaaccc ttggagaagt ggtatttaat actgccatga
     9061 cggggtatca agaaatttta accgaccctt catataaagg gcagattgtc ctgatgacct
     9121 ccacccaaat cgggaattat ggggtcaccc cggaggataa tgagtcccat cgcgtctggg
     9181 cggaagggtt tattgtgcgt gaagcagcca gttgtcggag taattggcgg agcaggcaat
     9241 ccttcgagga atatttaatt gagcatcgta ttgtggcgat tcaagggata gacaccagag
     9301 ccctgacctg tcatgtccgt gatcacggct cacaaatggg agtcatttcc catttaagtt
     9361 tcgatgacga tgccttacgg gaaaaggcga aggccgttcc ctccctgatt ggacgtgatt
     9421 tggttagaga ggtcacctgt tcggaagctt acgaatggac cgagcgatcc atcgtgatgg
     9481 actcgcctcg tggaaaatca ccaattgttc ctcaggacat cgctcagaca cctttgaaac
     9541 tggtggtcat ggattttggc gtgaaacaga atattttacg aagccttgtg gatctgggat
     9601 gtcaggttcg agttgtgcca gcttcgacga cagccgagga aatccgtcgg ctgcatccag
     9661 atggtatcgt tttatccaat ggaccaggag accccgaagg ggttccctat gcggtagaaa
     9721 ccgtcaagca gctcttgggc tggaaaccgt tgctggggat ttgcttgggg caccaagtat
     9781 tgggattatc cttgggattg cacacacata aactcacatt tgggcatcat ggggccaatc
     9841 atccagtcat ggatcttcgc accagaaaga ttgaaattac ctcgcaaaac cataattttg
     9901 ccgttggcct tcctcacaag gaagatgggt gtacgacgtt gaagtctcaa caatttgact
     9961 ctgccgttgg acggatggaa attacgcatc gaagtgtgaa tgatgggtgt tgtgaaggaa
    10021 tggcgggaat agagagtccg attctttcta ttcagtatca ccctgaagcc tctccaggcc
    10081 cccatgactc atcgtatgta tttggacaat ttttggaaat gatgaaaggc cgtggataat
    10141 gataatgact cgtggcttcc tgattcttat tctatttttg gtcagcgggt gcattcacat
    10201 cgatctgctt cccggtggag gaaaactcca tgaagccact ctgtctggtg aaggagagga
    10261 taaggtattg ttgattgata tctctggcat gctcacgaca ggcaaatcat cgggtatctt
    10321 ggaggaaccc agtcttcccg cacgaatcaa agaggaattg accaaagctg aaaaggatga
    10381 acacgtcaaa gccatcgtcc ttcgaatcaa tactccagga ggatcggtca ccgcatcaga
    10441 tttggtctat catgagctga aagtctttaa gaaaaaacgc tcagtaccgg tcattgcctc
    10501 tataatggat ttgggtactt ccggcggtta ttacatcgcg atggcggctg accatatttt
    10561 tgcccatccc tcgactatca ccggaagtat cggggttatt atggtgacaa tgaatgccga
    10621 gggtctgttg gaaaaagtgg gggttcaacc agccgcgata gtttcgggtc cgaagaaagc
    10681 aatgggttct ccgtttcggc cgatgaatga tgaagagcgg gcaatatttc aaggggcgat
    10741 tgatcatttg tatgagcagt tccttgcggt ggttgaagag ggaagacccg gattgagcaa
    10801 ggagcacatt cgtacgttag ccgatggccg gatttttacc gccgatatcg ccagagacca
    10861 gggccttgtg gatgacatcg ggtatctgga tgaggctctc gatctggcca aaaaagaagc
    10921 gaaattggaa gcggccaaag tcgtgaccta taccagggga gggggaactc aacagaatat
    10981 ctactcgcaa ttcgatcctc ctcagattga gcctatcagg tttccccaag tcgatacgca
    11041 gtctcttttc aaggtattga cgggagggac accacaaatg ctgtacatgt ggatgccgta
    11101 aagtttgctg tttgctgatt tgaggaatgt tttgcctatg accaacgatc cacccgtttc
    11161 tggaaaccga tttcaatgcc tggtggcgag tacggtgtgt gcgcttctat tgctgagcgt
    11221 agcttgtcaa aaggctccag gaacagctag agatcaatta atttatattt cggaggaaaa
    11281 ggaaattgcc ctgggacttg cggcatttcg cgaggtcctg aagtcggcac cattaagcag
    11341 agatccggaa gtgacgctaa tggtgaaccg tgtggggcaa cgcattgcca agggagccaa
    11401 taaaccggaa tacgtctggg aattcgccgt cattgaggat gatgatcaag tgaatgcctt
    11461 tgccttgcct ggaggcaaag tggcagtgtt taccggaatt ttgaaataca caaaaacaga
    11521 agccgggctg gcgaccgtca tggcccatga agttgcccat gcgttgcaac ggcatggagc
    11581 cgagcgatac agtcgtggaa ttctggaaca aattggtcaa ctcggagctt tagctggcgc
    11641 cgccgcagga ggcgtcgacc caggtcttgc tattggagct atgagtgcct atggggtggg
    11701 tgtggccctt cccaattccc gtgaacagga aactgaagcc gattttatcg ggttgcaatt
    11761 gatggccaaa gccggatatg acccgcggga agcggtgccg ttttgggaac gcatgagcgg
    11821 gtgcccgaga aaaatgatcg gaaaattttg ctttcgtaca caaaatgccg ttccggaatt
    11881 tttatccact cacccttcgg atgtgacccg gattaaccga attgaagcct ggattcccca
    11941 agccatgaaa ttttatcatc ctgagggatt accgcctgga gtacctgaat ccccgctcaa
    12001 aaaaccgaca tctgaatcgg atttaaaatc ctgagagccc aacatgccac gaagaaacga
    12061 catccatcga attttattaa taggctccgg accgattgtc attggtcaag gctgtgagtt
    12121 tgactattct ggaacgcaag catgcagggc actgaaggag gaaggctttg aagtcatcct
    12181 cgtcaatagt aatcctgcca ccattatgac cgacccggat ctcgcggacc gaacctatgt
    12241 ggaaccgatt acggcagaag tggtggaggc gattttggag cgagagcatc ctgatgccct
    12301 gcttccgacc atgggcgggc agaccgctct gaatgtcacc atggaattag tgaagcgagg
    12361 gattctggaa aaacacggag tgcaacttat cggagcttcc tatgaatcga tccagaaggc
    12421 cgaagatcgg gaattattca aacaggcgat ggcacgtatt ggattgaacg tggcggcaag
    12481 cgggtcattt cgctcaagag aggaagcctt acagcttctt caaacgattg gatttcccgc
    12541 tatcgtccgc ccatcattta ctctgggagg agcagggggc aatattgcct ataaccggga
    12601 agaatttgat aagtacattg attgggctct tgtgaccagt cccgttggac aagccttgct
    12661 ggaacaatct cttctgggat ggaaggaata tgaattagaa gtgatgcggg actccaaaga
    12721 taatgtggtc attgtctgcc ccatcgaaaa ttttgatccg atgggtgtgc atacaggtga
    12781 cagtatcacg gtggcccccg ccatgacctt atccgataaa gagtatcagc acatgcggga
    12841 cgcggcgata cgtattattc gagaaatcgg ggtggatacc ggcgggtcca atattcaatt
    12901 ttccttgaat ccggacaatg gggccttgat ggttattgag atgaatcctc gcgtgtccag
    12961 aagttccgcc ttggcctcaa aggcaacggg gtttccgata gccaaaatcg ctgcgaagtt
    13021 ggccatcggg tataccctgg acgaaattcc caacgatatt acccaagtga cgaaagcctg
    13081 ttttgagccg accattgatt atgtggtcgt gaaaattcca cggtttacat ttgaaaaatt
    13141 tgatggggta gatcgagtgc tgaccacgca tatgaaatcc gttggagaag ccatggcact
    13201 cggaggcacc ttcaaagaag ccttgcagaa ggcgattcgg tcacttgaaa cagaccggta
    13261 tgggttgatg tcgttgcacg ggcttgatgg gaaaaaagac actgccggac cggatcatcc
    13321 actacatgac cgccttcaat ctcaggttcg aatccccact gccgatcgcc tttggtttct
    13381 ggctgatggg ttccgaatag ggatgtcggt agatgagctg tatcaattgt ctcgggttga
    13441 tcgctggttt ttacatgaaa ttgaagaact cgtagaattt gaagcccaat gggttcgcaa
    13501 aagcaaggaa gaatttaaga ccgatcgttc acatgacaac ccgaaaggat gccctgatac
    13561 tctgctgatt ctccttgaac aagcgaaggg ccttggattt tccgatcaac ggcttgggca
    13621 actcatcggg agagaagaag ataccattcg tcagtggcga aagagcaggg gatccggcta
    13681 tggcaccgtc tttaaaagag tcgacacgtg tggggccgaa ttcgaggcac atacccctta
    13741 tctctattcc acgtccggtt taacctgtga atcccgccct tcccaaaaaa gaaaaatcat
    13801 gatcttaggg ggaggcccga accgaattgg gcaaggcatt gaatttgatt actgttgcgt
    13861 gcatgccgcc atggccctaa aagaagaagg cattgaaacc attatggtca attgcaatcc
    13921 ggaaacagtc agtacggatt atgacatttc cgatcgcctc tattttgaac ccttgacgaa
    13981 agaagaggtc ttgcgaatta tcctggccga acgtccggat ggggtcgtcg tacaattcgg
    14041 tggacaaacg cctctcaagc tggccgttcc gttagagcaa gaaggggtaa gaattttggg
    14101 gacacaaccc gatgcgattg atcgcgcgga agatcgaaaa cggtttagtg atctattgat
    14161 tgaattgaat ctcctgcagc cgcaaaacgg taccgcacgg tcctcgcatg aagctcatat
    14221 tattgccgtc aatattggct atccagtcat ggtacgccct tcgtatgtat tgggggggcg
    14281 tgccatgcaa atcgtatatg atgctgacgc gttggatgaa taccttcagc ggcatgacat
    14341 ggacttggga agacatccct tgttaatcga tgattacctt gaagacgcca tagaagttga
    14401 tgtggatgcc attgcagatg ggacagacgt cgtggtggct gccatcatgg aacatgtcga
    14461 aatggcaggc attcattcag gagattcagc gtgttcatta cctgcccact cccttggtgc
    14521 cgatattctt gagacgatca atcgccaaac cattcttttg gccaaggagt tacaggtgac
    14581 gggtttaatg aatatccaat atgcggtcaa gggtggggcg gtctatatcc tggaggtgaa
    14641 tcctcgcgct tctcgcacgg tcccttttgt gagtaaggtc attggtgtgc ccttggccaa
    14701 acatgcgatg aaagttatgg ctggacgaac cttgaaagac ttaggcttta cgcagtcacc
    14761 gcttttttcg cacattgcca ttaaggaagc cgtctttcca tttacgaaac tcggtgtctc
    14821 tgatgtatta ttgggtccgg aaatgcgttc cacgggcgag gtcatgggga ttgatcgaag
    14881 cttcgggtgg gcgtttgcaa aatctcaagc cgcatcagga atgcctttgc ctttggcagg
    14941 gactgctctt ctcagcgtga aagatgggga taaacccgcc acacttgcta tcgcgcagcg
    15001 actgtcgcaa ctcggatttt cactgaatgc gactaagggg actgcggcct ttttgcggaa
    15061 acatgggatc accgtagtga cggtgaacaa acttaatgca gcccgccccc atatcgagga
    15121 tttactcaaa aacaaagagc tgtctttagt tgtaaatact gtaggtggtg cctcttccca
    15181 ggatgattcc gccccaattc gcaaagcggc tgtcttcggc gggattcctt actttacgac
    15241 cattcaagcc gcgcaagcag ccatatcggg aatagaggcg atgaagaaaa catccataga
    15301 ggttcgctgt ctgcaggaat atcattctgg attgccagcc gggtaagcca aataggaccg
    15361 aatgcgatga tggattatga accatccgag tttcaggatc taataaaggg gagtgtggtt
    15421 tcatgaaaat tcctatcaca aaaaaaggat atgaagcact gcaggcggaa ctggccaggc
    15481 tgcgacggga agatcgccct aaaaacattc aggccattac cgaggctcgt gaacatggtg
    15541 atctcaaaga aaatgccgaa tataaagccg ccaaggagca gcagcaattt attgatacac
    15601 ggatggctga gcttgagcat aaactcagca tggctcaagt ggtggaaata gccagcgggc
    15661 agagtgacac ggttgtgttt ggcgtcacag tctccattgt cagtttagag tctcaggagg
    15721 aaaaacgtta taccctggtg ggacaagaag aagccgatat caaaaatggt tctatttcgg
    15781 ttcaatcgcc tattgggcgt gcgctgattg ggcatcgggt cggagatatc gttgaagtgc
    15841 atcgacccgc cggggttatt gaataccaaa tccagtccat ttgttttgag gagctataat
    15901 catgacttcc cccatttcct tagttacatt ggtcactgat tttggagata tcgattattt
    15961 tgtcccaagc atgaagggcg taatgctcgg aattaattct caaatacgca tcgtcgatct
    16021 gacgcatcga atcccggcgc atgggattga acaggccgcg ttttttttga aatcgtgtta
    16081 tcaatatttt ccagatggaa cggtgcatgt ggtggtcgtg gaccctgctg tcgggagttc
    16141 ccgacgggca atcttggctt caacctccag acatttcttc ctggctccgg ataatggtgt
    16201 tctgagttac gtgctccaag aggaaacgtc ggttgaagtt cgatcgattg agaataaaca
    16261 ataccgatta gattctgcgg gcggaacgtt tgatggccgg gatttgtttg caccctctgc
    16321 cgcctggttg acgaaggggc aggttcctgg ttcttacgga cgactcattc atgattatgt
    16381 cacgcttccc ctagatcccc cacgtatgca acagcaggcc cttcatgggc gtattgtcta
    16441 tgtcgaccat tttggtaatg ccatgacgaa tatcaccctg gccgatatcg aaacatttcg
    16501 atcagtcacc aagaaggaat actccggttt aaaaattggg gatgcactca ttaatggaat
    16561 aaagacccat tatggagaag gcgccatggg aatcccggaa gccttaatca atagtaatgg
    16621 tcatgtcgag atttttgtga agcaagggcg agcggtggac cgatgtgagt tgcacgttgg
    16681 ccaaactgtt gaactcatct aggtttgaac tgagaaagat gggcgggatg aacgaacaac
    16741 tgaaatagcg agttttagaa tcgataccat ttgctcaagt tccttgacgc tggcggaaag
    16801 tggaggcatc agaatcataa ttgtgccgat gggacggatc aacattccca aggttctggc
    16861 agtcatggct attttctgac cgatgcgttc gtccgcaggg aacggttttt tattagctcg
    16921 atcttgaacc agttcaatcc cgaccatgta gccacattga cggatgtcgc ccacccaagg
    16981 atcgtcgctc aacgactcca gggcctttcg taattttggg attcttcgtt gaagctttgc
    17041 aagtgtgcgt tcgctcttga aaatgcttag gttagcaaga gctgccgcac agcccaaagg
    17101 atttcctgcg taactatgtc catggaaaaa agttttgcct tcatgccctt ctcccaaaaa
    17161 cgcgtcataa atggcggctg tggttaacgt ggcagccaag ggtagatacc ctcccgttaa
    17221 tcctttggca atggccataa tatccggagt tatttcttca tgctcacagg caaacattaa
    17281 acctgttcgt ccaaacccgg tggcaacctc atcggcaatg aataagacgt tatagcgcgt
    17341 gcatagatcc ctaacgcgtt tcaagtaacc tggaggggag ggaataatcc cggcgactcc
    17401 ttgcaccatg ggctcaagaa taaggcctgc aatttcttga tgtgatgtgt gcagaagctt
    17461 ttcaagagga tcgaggcagg caagctggca ttggggaaac tccatatgaa ggggacatcg
    17521 ataacagtga ggtggttcaa tggcgtggct tggaaagagg agggaagaaa aaggtctacg
    17581 gaatagttcg actccgctga ggctcacgct tcccactgta tccccatgat aggataggcc
    17641 tagatggata aatcgagttt tttgtggctg gggcgttgga cattgttgcc agtactggat
    17701 agccatcttt gccccaactt caacagccgt tgaaccgttg tctgaataaa agacttttcg
    17761 taagcctttc ggagccaggc gaataagtgc cttcgccaat tgaatggctg gtgggttgga
    17821 aagccctagt agcgtcgtat gagccacctg ggtcagttgc cgacggatgg cctgattaat
    17881 gcggggatgt tgatggccat ggacattgac ccagatcgat gctgtggcat caagataggc
    17941 attgccgtcc atgtcataga gataagatcc ttttccgcgt ctgataatga gaggagtctg
    18001 gccctcccat tcctgcattt gggtaaaagg gtgccaaatg tactcacaat cgtctttttc
    18061 taatgctgtg tgggacattt tttgatctct ggtccggtta aagacaataa actagcggaa
    18121 tccatcaatt tttgacaacc ttttagggtc acattacaat cacggtcact tcgaatgagt
    18181 aaagcattcc ctcaaacaca catccgcaat ttttcgatta ttgctcatat tgatcacggc
    18241 aaatccaccc ttgctgatcg gtttctggaa atcaccggtg ccgtgtcgcc acgtgaggcg
    18301 cgtgcccaat atctggacgc catggatctg gaacgtgagc gcgggatcac cattaaagca
    18361 catgcggtca caacgcggtt taagagctcg gatgggcaag actatcaatt taatctcatt
    18421 gatacgcccg gccatgtcga ttttgcttat gaggtatcgc ggagcctggc tgcctgtgaa
    18481 ggagcccttc tgttgattga tgccactcag ggggttgagg cccagaccat cgccaatgcc
    18541 tatctggcga tttcgaataa tttagtaatt attcccgtca ttaataaaat cgatttaccg
    18601 agtgcggatg tggaaggcgt aaaggtccaa attcgagatg tgttgggatt gtcgagtgag
    18661 gaagcctttt tagtcagtgc caaggatgga cgaggggtca aggaggtgct tgaaggtgtg
    18721 gtgaaggtca ttccggctcc aatcgggtcc attgatcaac ccctaaaggc cctgattttt
    18781 gactcctggt ttgacaatta tcagggagcc gtggtgcttc ttcgagtcat ggacggagaa
    18841 gtcaccccca atatgatgat taaattaatg ttttccggac gtgaatttga agtcctggag
    18901 gtgggcgttt tttctccgaa acgaaccaag gtaagtcggc tggggcccgg ggaagtcggg
    18961 tatatttgtg ccggaatgaa agagctatcc gacaccaaaa ttggtgatac cattacggat
    19021 tcaaagcgcc ccacaagcgt tgcccttccc gggtaccggg atgtcaaacc ggtagtattt
    19081 tgcgggttat acacgacgga taatgccaag tttgaggatc tgcgggattc tctagagaag
    19141 ttgcaactga atgattcctc ttttgtctat gaaccggaaa cctccctggc tttgggattt
    19201 ggattccgat gtggatttct agggctcctc cacatggaaa ttattcgaga gcgcttggag
    19261 cgggaatatg gactggatct gatcagtacc gccccaactg tcgtctatcg tgtattaacc
    19321 accaaaggcg aaacattggt catcgataat ccatcaaaac tcccggatcc ttcgcagatc
    19381 gaacgaattg aagagcccta tattaaagcc acgatgatta cgccggagcg gtatatcgga
    19441 aatgtccttc aactgtgcca agagcgtcga ggcattcaaa tcgggctgca atatctggat
    19501 ccaacccggt ccatgttaac ctacgaaatc ccgctcaatg aaattgtgtt agacttttat
    19561 gatcgactga aatctcggac tcaagggtat gcctcgttag actacgaatt gctgggctat
    19621 cgcgagtctg atctggtgaa attggattta ttgctgaatg gtgagacggt ggacgcgctc
    19681 tccattatcg cccacaaaga taaagttcaa agtcgggggc ggcaattagc cgaaaaaatg
    19741 aaagaactca ttcccaagca aatgtttgaa attgtca
//
LOCUS       DAORAQ010000041        19574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_500_length_19574_cov_68.605103, whole genome shotgun sequence.
ACCESSION   DAORAQ010000041 DAORAQ010000000
VERSION     DAORAQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 19574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19574
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_19574_cov_68.605103"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKM72_05855"
     CDS             complement(<1..233)
                     /locus_tag="PKM72_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60343.1"
                     /translation="MNFDSPLVNPTLLPSIMSTVHSEPLFQHVTIIGIGLLGGSLGLA
                     LKKQGLAKMVVGIGRRQENLELAVQMGAIDQFFL"
     gene            complement(223..1236)
                     /gene="aroF"
                     /locus_tag="PKM72_05860"
     CDS             complement(223..1236)
                     /gene="aroF"
                     /locus_tag="PKM72_05860"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNP60344.1"
                     /translation="MVIVLKPEATDQDIDHLIDRLRSLGMTTQITKGKERTIVAVLGD
                     DRVLQGQPLSVFSGVESVTPILAPWKLVSREFQKQDSVIDVAGVKVGGSQIVVMAGPC
                     AVEKLEITVGIAQEVAKAGARILRGGAYKPRTSPYSFQGLGQEGLDFLAAARQQTGLP
                     VVSEILDARDLGHFLEKADVIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKELL
                     LSAEYIMSRGNRNVMLCERGIRTFETQYRNTLDLSAIPTLKEMTHLPVIVDPSHATGN
                     WKLVAPMAKAAIAAGADGLLIEVHSNPECALCDGEESLKPSRFTELMNDLQKVAQAVG
                     REL"
     gene            complement(1258..2343)
                     /gene="hisC"
                     /locus_tag="PKM72_05865"
     CDS             complement(1258..2343)
                     /gene="hisC"
                     /locus_tag="PKM72_05865"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNP60345.1"
                     /translation="MPLHVHPTIASLTPYSPGKPLDELERELGITQAIKLASNENPWG
                     PSPNALQVLAEAAASLHRYPDGGAHYLRQALGERLKFPEDQVLVGNGSDEIISLLVRA
                     FLSPGDEAVMADLTFVMYKLSVLGGHGVPVEVPLKNWAHDVAAMVDAVTDRTRLFFLC
                     NPNNPTGTMLTAREIDGFLSRLPDHVVVVFDEAYYEYVRHPDFPDSLQYVREGRPVVV
                     LRTFSKIYGLAGLRVGYGLTTKEIAGYVNRVRPPFNVNSLAQEAARAALSDDDHVAKS
                     RAMNEAEMTFLEEGLTTMGLEPVPSQANFLYFNAGIDGKLAYEALLREGVIVRHIRGP
                     MLRVTIGQPSENRRFLDSLTRVLSTVL"
     gene            complement(2480..3562)
                     /gene="pheA"
                     /locus_tag="PKM72_05870"
     CDS             complement(2480..3562)
                     /gene="pheA"
                     /locus_tag="PKM72_05870"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNP60346.1"
                     /translation="MELPEDMQQCRQEIDRLDDDILNILNERSQYVIKIGHLKKQQDS
                     SAHLHTPGREAAIVERLMRKNPGPFPSEALRHLYREIMSASLSLEGTQTVAYLGPPAT
                     FTHLAAMRKFGGSADYVSVNSIKDVFDEVERGRMRFGVVPIENSTEGVVNHTLDLFVD
                     SPLLIYGEVMLEVSHHFLSKAEKIDEIKTVYSHPHAIAQCRNWLETNLPSVNFAEEPS
                     TARAAERAVNDPTAGAIASELAAQLYGLNILRSRIEDNINNFTRFLILSQKGAEPTGR
                     DKTSIIVSAKDRVGALYDLIRPFSSHGISMTKIESRPTRRKVWEYLFYMDLEGHQDDD
                     RLKKALEEVRSRCLMMKVLGSYSSHQ"
     gene            complement(3718..4677)
                     /locus_tag="PKM72_05875"
     CDS             complement(3718..4677)
                     /locus_tag="PKM72_05875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP60347.1"
                     /translation="MKNMLKEYALKVLKSLPARFSLSVNWNSTEGYMLGYAKEILRTL
                     ENDQSVTTRQDAITGLRQLSLEEFGLVLLSMPDPEYPKLSRLLPAMASNEVQRNWTGN
                     HGVELLKQTTAFVRALSYNYTRVTGRSLDDATILDFGCGYGRIAQLLYYFTAEEKVFG
                     VDPWEQSIELCRESGLSTNFMVSDYLPVSLPVGTVKFNLIYAFSVFTHLSQRAMSASL
                     NTLRKYIADDGMLAITIRPVENWEHDPHTSSAQKAALAVRHREEGFAFNPHNRAPIDG
                     DVTYGDSSLTLEWITSTFPEWTIKATDRCLSDRLQRYVFLVPR"
     assembly_gap    4834..4933
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5353..6066)
                     /locus_tag="PKM72_05880"
     CDS             complement(5353..6066)
                     /locus_tag="PKM72_05880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP60348.1"
                     /translation="MPGMFKSPNKAEDNILSDLGPLYHNYSFFGVNNVQLPGIYELNQ
                     QAKAPIIIAYIAYAIAKSKKHALDRVSLSELFCADGFYAMVASRLGCNRSIGIDNDRD
                     KHFEHANVIAERLNLHGVEFEKEEITPNSKFISTDVVVNVGGLYHVDAPDKILDLSYG
                     MANKYLIVQSVVSLKTESEDYFEAPAPGWTWGNRYSRKSFDKMIKKICPKIIDQHFNE
                     LEGNNRLEDRGSVYYLIEK"
     gene            6309..6524
                     /locus_tag="PKM72_05885"
     CDS             6309..6524
                     /locus_tag="PKM72_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60349.1"
                     /translation="MPENFHGLEQARAWPQKNFTPKISLGNSGTFNIFADRVWQISEQ
                     NNQKGLRGHKVFTSFAHTLTQKRGTIE"
     gene            complement(8041..8829)
                     /gene="panB"
                     /locus_tag="PKM72_05890"
     CDS             complement(8041..8829)
                     /gene="panB"
                     /locus_tag="PKM72_05890"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNP60350.1"
                     /translation="MTIPEFVQQKKQGKKLTVVTAYDALFTRVVEQAGLDVILVGDSL
                     GMVVQGKPNTLSVTMEDMLYHTRLVAQAAQRALVIGDMPFMSYQASVEDAVRNAGRFL
                     QAGAAAVKLEGGAPVVDRVEAMTRFGIPVMGHLGMTPQSVNQSGGYSVKGKAQEEAET
                     IVSDAKALEAAGAFAVVLECMPIGLAQSITEALSIPTIGIGAGQGCDGQVLVLYDLLG
                     LFDDFVPKFVKPYAHLKADALQALRRYKEEVECGKFPTDAESYH"
     gene            complement(8861..9718)
                     /gene="folD"
                     /locus_tag="PKM72_05895"
     CDS             complement(8861..9718)
                     /gene="folD"
                     /locus_tag="PKM72_05895"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNP60351.1"
                     /translation="MPAQIIDGKALAQAIREGIAEDVRLFEKDTGIKPGLAAVLVGDD
                     PASAVYVRNKRIACEKAGLYPQEHLLPASTPQESLLKLVHQLNADPQIHGILVQLPLP
                     SGMDSQAILHAVSPEKDVDGFHPVNVGRLVTGDPGFIPCTPKGVIQMIDSTGQDIAGK
                     RVVVIGRSNIVGKPVAMLLLHRHATVTICHSRTKDLPGVVREADIVIAAIGKPLFVTP
                     DMVKEGAIVIDVGINRLADGKLVGDVDFGRVKERSGWITPVPGGVGPMTIAMLLQNTV
                     ESAKRVAGK"
     gene            10022..10906
                     /locus_tag="PKM72_05900"
     CDS             10022..10906
                     /locus_tag="PKM72_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60352.1"
                     /translation="MANLAFPQSMIADGGQVYFIANANGDPGTRENKGFISKVTPEGK
                     MIDLHFIQGGQKSVILNSPKGMALVGATLYVADLDAVRGFDKNTGETLHTISFAQFHS
                     QSLAGLAADPLGGLYVSDSDSNTIYHIDEAAHDHTVTVFAQDESLSHPRGLTIHPRNG
                     HVVGVGWEDGKIFEIDEHGTIQELFANTFFTGGFYNLDGIDFDKYGTMYVSDLTVGKV
                     WRILANHKKEVIAEFLLSPSGIGVDRVNHVIMVPYLYVNGAEINGLERPSNDNPNKKP
                     RSWSDYGLGWLKKDAANE"
     gene            10899..11570
                     /locus_tag="PKM72_05905"
     CDS             10899..11570
                     /locus_tag="PKM72_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60353.1"
                     /translation="MSKCLYCHQRKGKRTCPALGGMICSQCCGTNRITNISCPTSCVF
                     LESNDDYQQKRVGNRFELERRGFYRQLLDLGGERATEIFYVFEALAYRFFQDRRDAQD
                     VEVLEGLQSLRRSFSLIQIPDSGIPAFGEELRKEFKVFGERQPLESHLVTNVLDQAHI
                     FIQEFSGSGLRSHRFLHGLLGYIRERHPDVAEQLARQTGAGGRIIIPSGFDYAPDPPS
                     TEPVS"
     gene            complement(11612..12238)
                     /locus_tag="PKM72_05910"
     CDS             complement(11612..12238)
                     /locus_tag="PKM72_05910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNP60354.1"
                     /translation="MRVVLQPRTLASALRQLMMVIGLSTIVTAGGSLVPTGAAAEEPS
                     NADTKAPHVLIKTKFGEMEAVLFPDLAPKHVESFLKLVKSGFYNGTIFHRIIPGFMVQ
                     GGDPLTKDPANRSRFGTGGPGYTVPAEFNRVAHEKGILSAARTADPNSAGSQFFLMVD
                     KAPHLDGQYTVFGEVVKGLEVLDTIVGQPRDLKDNPLERIEMTIDVMP"
     gene            complement(12235..13005)
                     /gene="rnc"
                     /locus_tag="PKM72_05915"
     CDS             complement(12235..13005)
                     /gene="rnc"
                     /locus_tag="PKM72_05915"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNP60355.1"
                     /translation="MAALAFEDLQESLEYVFKQQALLCEALTHRSYSQTQPQAVSPHN
                     ERLEFLGDAVLGLIVSESLAAMFPSGTEGELSKIKARLISRSTLSKAASRLRLGQWLR
                     LGRGEEASKGREKISLLANVLEAIIGAVYLDGGLEPARAFIQKVLTTEFSSLENSPVS
                     SAGWDGKSRLQEWTHKQFGVSPQYRLVQESGPDHQKVFAVTVEIQGKIMGQGEGRTKK
                     EAEQCAAAKALAQSEIHVSGELGHPIKKFNRLAMEEEL"
     gene            complement(13176..14429)
                     /gene="fabF"
                     /locus_tag="PKM72_05920"
     CDS             complement(13176..14429)
                     /gene="fabF"
                     /locus_tag="PKM72_05920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNP60356.1"
                     /translation="MSEWTPRRVVVTGLGLVTPLDIGVSNTWEKLCKGQSGIGPITRF
                     DASQYSVQIAGEVKNFDPSTFIEKKEIKKMDTFIHFAIAASQEAVDDAKLVVNPDEAD
                     RVGVYIGAGIGGLPAIEHYHKVLLERGPDRVSPFFIPMVIINLASGQVAIRFGAKGPN
                     ACTVTACATGNHCIGDGFRLIQRGEADVMLVGGAESTICPTAVAGFAAAKALSRRNEE
                     PKRASRPFDKDRDGFVIGEGAGVLILEELEHAKSRGARIYAEVIGYAMNSDAFHITAP
                     PDDGAGAVTCMERAIQDAGIAKESVGYINAHATSTFADRIETQVIKKVFGDLAYSIPV
                     GSTKSMTGHLLGAAGGIEAVFSIMAMHQGVIPPTINLETPDPECDLDYIPTHARSCTV
                     DVAISNAFGFGGVNACLVFRRWKGE"
     gene            complement(14551..14781)
                     /gene="acpP"
                     /locus_tag="PKM72_05925"
     CDS             complement(14551..14781)
                     /gene="acpP"
                     /locus_tag="PKM72_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNP60357.1"
                     /translation="MAVEERVKKIIVEQLGVEEDDVVPEAKFVEDLGADSLDTVELVM
                     ALEEEFEIEIPDEDAEKIQTVSAAIEFIKEKV"
     gene            complement(14841..15587)
                     /gene="fabG"
                     /locus_tag="PKM72_05930"
     CDS             complement(14841..15587)
                     /gene="fabG"
                     /locus_tag="PKM72_05930"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNP60358.1"
                     /translation="MLLTEKIAVVTGGAQGIGQAIATTLAREGADVVVADLDAERCQE
                     TVDLVQQSGRKALAVSVNVGEWEQAKGMMDRVLKEWGRVDILVNNAGITRDGLLMRMK
                     EEDWHAVLQVNLTGTFFCAKAVLPSMSKQRSGRIVNIASIVGAIGNMGQANYAASKAG
                     VIGLTKTIAREYASRNITVNAVAPGFIDTAMTQHLSPEIKEGLLNQIPLKRLGQPSDV
                     ADAVCFLSSEKAGYISGHVLHVNGGMHMAS"
     gene            complement(15625..16557)
                     /gene="fabD"
                     /locus_tag="PKM72_05935"
     CDS             complement(15625..16557)
                     /gene="fabD"
                     /locus_tag="PKM72_05935"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNP60359.1"
                     /translation="MPVFGFLFPGQGSQSIGMGKPLFDSSPSVQALYKEAADILGYDV
                     GGLCFEGPSEQLNQTEYTQPALLVTSLAAFQLVREGPLLPAAVAGHSLGEYTALVAAG
                     ALEFREAVNLVQKRGRYMAEAVAPGSGLVAAILGLSEKDVRSVCQEAGSVGVVAPANF
                     NSPGQTVIAGEKAAVEHAAELAKARGAKRVMPLPVSVPVHTPLMQVAADRLKKDIDSL
                     KWSDLNVPLVNNVEAKALVSAGEVRESLVRQLPSPVRWQETIQAMALMGITHFVEIGP
                     GKVLTGLVKRIVPEATTWNVMDQKSYAHVLSHCS"
     gene            complement(16635..17450)
                     /locus_tag="PKM72_05940"
     CDS             complement(16635..17450)
                     /locus_tag="PKM72_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP60360.1"
                     /translation="MSGTTSWGENDWALILGASSGFGEAAALELANLGLNICGVHLDR
                     KSTQPNAERIASQIRSIGREVLFFNVNAADPEKRADILKALKQRAEESGKPICVRVLL
                     HSLAFGTLKPYIAESHAEAINKAQMDMTLDVMANSLVYWTQDLVAQKFLGSGSRIFSM
                     TSAGGARVWRTYGAVSAAKSALESHTRQLALELAPLGITVNSLMAGVTDTPALRKIPG
                     SDEMLTLAKRKNPSSRLTTTQDVADALGLLSHPKAHWITGNVICVDGGEFIVD"
     gene            complement(17573..18547)
                     /locus_tag="PKM72_05945"
     CDS             complement(17573..18547)
                     /locus_tag="PKM72_05945"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNP60361.1"
                     /translation="MKSVILGTGAYVPKRVLTNADLERMVDTSDTWITERTGIRERRV
                     VEPGQACSDLAVEAAQRALMAADVAASEIDLILLATCTGDSPLPSTACLIQHRLGAHR
                     AAACDISAACCGFIYALAIADAYVKSGMRHVLVIGSEVMSAITDWTDRNTCVLFGDGA
                     GAVVVGQGTEGSGILSTHLHANGGLSDMIQVPGGGSREPASKEVLEEKRCFIKMKGNE
                     TFKIAVKSMEEATKEALEANKLEIGDIDLFIPHQANMRILNAVGQRLGLAREKLMINL
                     DRFGNTSAASIPLALDQAVQEGRIHKGSVVLLAAFGAGLTWASTVIRW"
     gene            complement(18569..>19574)
                     /gene="plsX"
                     /locus_tag="PKM72_05950"
     CDS             complement(18569..>19574)
                     /gene="plsX"
                     /locus_tag="PKM72_05950"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNP60362.1"
                     /translation="TKPMKIAVDAMGGDHGLSPNVEGAIQATRESSLSIILVGDEPSL
                     QSHLDKLGGTKAGIEIFHAPQVVDMHESPALIARKKRDSSIWKATELVKNKQADALVS
                     AGNTGATMVSAFFLLGLIQGVERPAIAATLPTRQGKAIMLDVGATVDCTAKQLFQFGI
                     MGHEYGRHLLGNDRPRVGLLSIGEEDTKGNEVTKETFKLLKDSPINFIGNVEGRDVYS
                     GNADVIVTDGFIGNVALKISEGLADAIKKMLMKEIAQSALGRLSYLFVAGPLLRLRRK
                     TDYAEFGGAPLLGVEGISMICHGRSSSKAIKNAILRAQSLAEGGLIEEIRGDIAHGCR
                     "
BASE COUNT         4961 a         5314 c         4309 g         4890 t
ORIGIN      
        1 agaaaaaatt ggtcaatggc acccatttga acggctaatt caagattttc ttgtcgacga
       61 ccaatcccca caaccatctt ggctaagccc tgtttcttaa gggcaaggcc gagagagccg
      121 ccgagcaggc ctatcccgat aatggtcacg tgttgaaaga ggggttctga atggacagtc
      181 gacatgatgg aaggcaaaag tgtcggattc acaagaggag aatcaaagtt cacgaccgac
      241 agcctgcgcc accttttgta aatcattcat gagttcggta aaccggctgg gcttcagcga
      301 ttcctctccg tcgcacaagg cacattccgg attggaatgc acttcaatca acaagccgtc
      361 agcaccagcc gcaatcgccg ctttagccat aggagccaca agtttccaat tgcccgttgc
      421 gtgactcgga tccacaatga ccggcaggtg agtcatttcc tttaaagtgg gtatcgcact
      481 cagatcaagc gtattgcgat actgagtctc aaaggtgcga atccctcttt cacacagcat
      541 gacattccga ttgccacggg acataatata ctccgcggat aagagtaact ctttaatcgt
      601 ggccgacagc ccacgcttca ataagacggg tttgtcatag gcgccaacct cttttaagag
      661 ttcaaaattc tgcatattcc gggcgccaat ttggatcaca tccgcttttt ctaaaaaatg
      721 tccgaggtca cgtgcatcca atatttcact cacaacagga agacccgttt gttgcctagc
      781 tgcagccaaa aaatccaatc cttcctgtcc caaaccctga aaggaataag gcgacgtgcg
      841 aggcttatac gctccgcccc gtaaaatgcg ggcgcctgcc ttcgcgactt cttgagcgat
      901 accaaccgta atttctaatt tctccaccgc acagggacca gccataacca cgatctggct
      961 tcccccaact ttcacgccgg ccacgtcaat gacagaatcc tgtttttgaa attcacggct
     1021 cactaacttc caaggagcca gaatgggagt cacgctttcc acaccggaaa acacgctcaa
     1081 tggttgtcct tgtaacaccc ggtcatcgcc gagcactgcc actatggtcc gttctttccc
     1141 cttcgtgatt tgagtggtca tccccaacga acgaagacga tcgatcaagt gatcaatatc
     1201 ctgatcagtg gcttcaggtt ttaagacaat aaccataaat gaatcagaat ccttgcacta
     1261 caacactgtg gagaggactc gagtcagaga gtcaaggaaa cgacggtttt cggacggttg
     1321 gccaatggtc acccggagca ttggcccacg aatatgccga acgatcacgc cctctcgaag
     1381 caacgcctca tacgcaagtt ttccgtcaat ccctgcattg aaatacaaaa agttggcttg
     1441 actgggaacg ggctctaagc ccatggtggt tagtccttct tccagaaacg tcatttcggc
     1501 ttcattcatg gcccggcttt tcgccacatg gtcgtcatcc gaaagagctg ctcgtgcggc
     1561 ttcttgagcc aagctattca cgttgaacgg ggggcgaacc cgattcacat atcctgcaat
     1621 ttctttggtc gtcaacccat atcccacgcg caatcctgcc aatccataaa ttttcgaaaa
     1681 cgtccgtagt accacaaccg gccgcccttc acgaacatac tgcaagctgt ccggaaaatc
     1741 gggatgtcgt acgtactcat agtacgcctc atcaaatacg acgaccacat gatcaggaag
     1801 ccgggagaga aatccgtcaa tttcccttgc cgtcagcatg gtacccgttg ggttgttcgg
     1861 attgcagaga aaaaagagcc gtgtgcgatc agtgaccgcg tcaaccatgg ccgccacatc
     1921 gtgggcccaa tttttcaaag gcacctctac cggcacacca tgccctccca acacagaaag
     1981 cttgtacatc acaaaggtca gatcggccat taccgcttca tcacccggtg agagaaatgc
     2041 gcggactaaa agactgatga tttcatctga cccattgccc accagaacct gatcctcggg
     2101 aaatttcaac cgctcgccca atgcctgccg aaggtagtga gccccgccat ccggataccg
     2161 gtgaagcgag gctgctgcct ccgccaggac ctgcagcgcg ttcggggaag gaccccaagg
     2221 attttcatta gatgccagtt taattgcctg ggtaattccc agttcccgct ccaattcatc
     2281 caagggtttc cctggagaat acggtgttaa cgaggcaatt gtgggatgga catgcaatgg
     2341 catgggctta cattctttct cttgtgaaca tgaaggaaaa atccagaaaa tttcacgtct
     2401 acctgggcct gggacgaacg tttgcggaag ccccttcccc gatctccatt cctcaaggac
     2461 aaacagcgag catcaaacct tactgatgcg aagaataaga ccccagaacc ttcatcatca
     2521 aacaacgtga ccggacttcc tccagggcct tcttgaggcg atcatcgtcc tgatgacctt
     2581 ccaaatccat ataaaacaga tattcccaga cttttcgtct ggtcggccgt gattcaattt
     2641 tggtcatact aatgccgtgg gatgaaaacg ggcggatcag atcatacagg gctccaaccc
     2701 ggtctttggc cgagacaatg atcgacgttt tatcccggcc ggttggctca gctccctttt
     2761 gggataaaat gaggaacctg gtgaaattat taatattatc ttcaattcgt gatcgcagaa
     2821 tgttcaatcc atacaactga gctgccaatt cggaggcaat ggcccctgca gttggatcat
     2881 tcaccgcccg ctcagcggcc cgagccgtac tgggctcttc cgcaaaattc accgaaggca
     2941 agttggtttc gagccagttt cgacattggg caatggcatg cggatgagaa tagaccgtct
     3001 tgatctcatc tattttttcc gccttagaaa gaaagtgatg ggacacttcc agcatcactt
     3061 caccataaat caacagaggt gagtctacga acaaatctaa cgtatgattg accacccctt
     3121 ccgtcgagtt ttctatggga accacgccaa accgcatacg tccgcgttcc acttcatcaa
     3181 acacatcttt gatgctattg accgatacat agtcggccga tcctccaaac tttcgcatgg
     3241 cagccagatg tgtaaacgtg gcaggaggcc ccaaataggc gaccgtttgg gttccctcca
     3301 gagagagaga cgccgacata atctcccggt aaagatgacg aagggcttca ctgggaaacg
     3361 gtcctgggtt tttgcgcatc aaccgttcca cgatcgccgc ttcccgaccg ggcgtgtgaa
     3421 gatgggcaga cgaatcctgt tgttttttca gatgcccgat tttaatgaca tattgagatc
     3481 gctcattcaa tatattaaga atgtcgtcat caagccggtc gatctcttgc cggcattgtt
     3541 gcatatcttc tggaagctcc atgagactca tcctttatcc tggaaaatct gcaatttagg
     3601 caggagcata caaggaatac catgctgtgg caagcaagcg ttttcccctt tcaacttgcg
     3661 gctttcctat atacccagag ccacattttc cagccacaaa cgcaggagct tccccgatca
     3721 gcgcggcacc agaaagacat accgctgaag acgatcgctc aaacaccgat cagtcgcttt
     3781 gatcgtccac tctggaaaag tcgaggtaat ccactccaga gtcaacgagg aatccccata
     3841 ggtcacgtca ccatcaatcg gtgcccggtt gtgggggttg aaagcaaagc cctcctcccg
     3901 atgtcgaacg gctagggccg ctttctgcgc ggatgatgtg tgagggtcat gctcccagtt
     3961 ttcgacaggg cgaatagtaa tggctaacat gccatcatcc gcaatgtact tccgcaaggt
     4021 gttcaacgac gcgctcatcg ccctttgaga caggtgggtg aacacggaga aggcataaat
     4081 gagattaaac ttcaccgtcc cgaccggcaa ggacacaggg aggtagtcgg ataccatgaa
     4141 gtttgtactg agacccgact cccggcaaag ttcaatggac tgttcccagg gatcgacccc
     4201 gaagactttt tcctcggcgg tgaagtaata gagcaattgc gcgatacggc catacccaca
     4261 tccaaaatcc aagatagtgg catcatccag agaccgcccg gtcactcgtg tgtagttgta
     4321 actcaatgca cgcacgaacg ctgttgtttg cttgagcaat tcgactccat ggttccccgt
     4381 ccagtttcgc tgcacctcat tactcgccat ggcaggaaga agtcgtgaaa gcttggggta
     4441 ctcagggtcg ggcatggaca gcaacaccag tccgaattcc tccagactga gttgtcgaag
     4501 gcccgtgatg gcatcctggc gagtcgttac gctttggtca ttttccaatg tacgcaaaat
     4561 ctccttggca taccccagca tatagccctc ggtcgaattc caattcacag acagtgagaa
     4621 cctggctgga agactcttca gcactttcag ggcatattcc ttcaacatat ttttcatgaa
     4681 cacatttacc gtccgttgaa aatttgccgc aaaaaatgat ccccgccgca agcggacggg
     4741 tcattatgaa tgtactcgct cagacgaata gtttattacg ccgcaggcag cgggaaatat
     4801 accctcaagg gatttaactt cttctttata ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnncgccgca ggcagcggga aatataccct caagggattt aacttcttct
     4981 ttataccgcc ggagagcctg caaggcatcc gcctttagaa tgggcatagg ggttaacaaa
     5041 ttcagagatt ccagtcattg aataggccga accagtcgtt gaaaaccaag accaatcctc
     5101 aagccctgga gaattattta gtcctgatct gtgatctcga cacggagttc tcgaccttct
     5161 gaatttgatt tgagcagtgg attgaagctc ttccttgcca aacccttgat aagagccctt
     5221 ggtaggatgg attgaagttt cccttcaaag ttgagatatg cactaagaac tcttacgact
     5281 caagtgcggc aacacgtggc gtggctctca tctaaacgtt gtgaccaatt tagatgcttt
     5341 cattacataa acctattttt caatcaaata atacacactt cctctgtctt caagtctatt
     5401 attgccttca agctcattga aatgctggtc gatgattttt gggcagattt tcttgatcat
     5461 tttatcaaac gattttcgac tatatctgtt tccccatgtc caacctgggg ccggagcttc
     5521 aaaataatct tcactctctg tttttaaaga aacaacactc tgaactatta ggtatttatt
     5581 tgccatcccg tatgataggt ccaatatttt atcgggggca tcgacatgat aaagacctcc
     5641 cacatttacc acgacatctg ttgaaatgaa ttttgaattt ggcgtgatct cttctttctc
     5701 aaattctacg ccatgaagat tcagtcgttc ggctatcaca ttagcatgct caaaatgctt
     5761 atccctatcg ttatcgatgc caatgcttct attacagccc agcctggagg caaccatagc
     5821 gtaaaaacca tccgcacaaa acagctctga taaagatacg cggtctaagg catgtttttt
     5881 agatttagca atagcataag caatataggc gatgataatc ggtgcttttg cttgttgatt
     5941 taattcgtag atacctggca actgaacatt atttacccca aagaacgaat aattatgata
     6001 cagcgggccc aaatcagaaa gaatattatc ctcggcttta ttgggggact tgaacattcc
     6061 aggcatacgc acaatctcct tagttgaaat gattgacctt tcgcctatcc tttgagttca
     6121 tgagcacccg agcccgaatg gcggagaaaa ccgacaagca tagctatcca gtgctttcct
     6181 ctaaagaaaa gctagacata accggccttg ttaattctga tttcttaatc atccatagct
     6241 ctcatcgctt tgttcaaaaa tcacctgcca cacgacaaac aagcattaca tagtactcaa
     6301 tgaccacaat gccagaaaat ttccacggac ttgaacaggc aagagcttgg cctcaaaaga
     6361 actttacccc aaaaatttcg ttgggtaaca gcggcacatt taatatattt gcagataggg
     6421 tttggcaaat ttcagaacaa aacaatcaaa aaggccttag gggtcataaa gttttcacat
     6481 cttttgctca cacacttacc caaaaacgcg gcacaattga ataatagaga cttcaaatcc
     6541 cccccgcaga gtttgtcgac ctcctgaatg tgattcgaac acaggattgt taccatgcca
     6601 ggatggctat tctttttata atgtttgtcc ctttgcaagt gcacagaggc gtccagactt
     6661 aggcatcagt tgaaaattcc atttagttcg ttttctataa ggtaattcgc cagagaaaac
     6721 aacgatagct tctatctcgt tatctgtgag cgccggagag catattttgc caagcattta
     6781 agcagccgtc ctgttaaaaa gccggaacga gatgctttgt ggcatatcac cagcgatacg
     6841 cgtccgcagg tcacggtttt tcgcttggac accggggtgt ttttatggca cggccccctg
     6901 aagattgatg ccaataaagg caaatatcct gaagggcaat ttgatctcct tctggctttt
     6961 gcacctgtcg cataagcttc tttctaaatt tctctcactg cttattattg tcgccataga
     7021 ccagataaac agccagatag tatcgacctt cacggacaaa ggatgtcatt tttgtaagtc
     7081 atttctgata tccctaggct ttgcattcac gggtttcact tccataacga tcaagttgtc
     7141 gtccattccg ccagacttgt gtactagtaa atccggcttg gttttgccca aatcagcttt
     7201 acttagaatc gggtgcactg ccttgtcaac ttgtcctcca agcgaatacc caaaggtatc
     7261 accggtaact actctcaatt gatgatacag ctcatagcag tacacgcgtt ctcggtggat
     7321 cgggttcgct tggccaggaa ccggaagcag catctattgg tctggaattt ctgctgtggc
     7381 ctctgcgagg cagactagga attgatggca tgcgtcctct tccactttcg aatttggcat
     7441 catgatgtaa ttcaagcggt agcctccaaa cagtgcgctt cagaaccttt caggctaaca
     7501 ataattaagt tcatccacgt acggtgcgaa tctgtgattt cctgtccatt taacctctca
     7561 tcgactcttc tgaaaataag cggcaatctc tgcggtttca gtcaatggct gttcatacgg
     7621 gcatgtccta gggcatatta ttcggatcag catcccacca cctgatgaat ggtttgtcca
     7681 tctatacgac tgcgaacgct tgccgatccc gcaggacacc aagcgccaat cgaacgcttt
     7741 agaatggtcg tggggagatt tttggtgaac tggcgagtta agtcaagttg cctgtgttct
     7801 ttggcttctc tttttgacca tgtctcttgg gtttggtgaa ttggggaatt ctagatgggg
     7861 agtttggggt gaaaaaatga aagataagat tgatggcacg cgatgaggat gagtcggaac
     7921 cgaaggctat cttgccaggc ttatggagag cggttgttct ggaagcatta agggtaggga
     7981 ataggtaaat ttgttccccc ggaaaacctt gaagagaagg gggtgaaaaa attaatttat
     8041 ttaatgataa ctttcagcgt cggtggggaa ttttccgcat tcaacttctt ctttatagcg
     8101 ccggagcgcc tgtagggcgt ccgccttcag atgggcatag ggtttgacaa atttggggac
     8161 gaagtcatcg aagaggccca acaggtcgta gagcaccagc acctgtccat cacatccctg
     8221 cccggcgcca atgccaatgg tcggaataga aagggcttcg gtaatacttt gcgccagccc
     8281 gattggcatg cattccaaca ccaccgcaaa tgcccccgca gcctcaaggg ccttggcatc
     8341 gctcacaatc gtttccgctt cttcctgcgc cttgccctta actgaatatc cgccagattg
     8401 attgaccgat tgtggggtca tgcccaggtg acccatgacg ggaataccaa accgggtcat
     8461 ggcctccacc cgatcgacca ctggcgcacc accctcaagt ttcacagcag ccgcccccgc
     8521 ttgcagaaaa cgccccgcat tccgcacggc atcttccacg ctggcctggt aggacataaa
     8581 cggcatgtcc ccaatcacca gcgcccgttg ggcagcctga gccaccagcc gcgtgtgata
     8641 gagcatgtct tccatggtca ctgataaggt gttgggcttg ccttgcacga ccatgccaag
     8701 ggaatctcca accagaatga cgtcaagccc ggcctgctcc acaacccggg taaacagcgc
     8761 atcgtaggcc gtgacgaccg tgagcttttt accctgtttc ttctgttgga caaattcggg
     8821 aatcgtcatg tgtgccgatt tcttttgtat gcagtgaggg ttatttgcca gcaacccgct
     8881 tcgcggattc caccgtgttc tgaagcagca tagcgatcgt catcgggcca acgccaccgg
     8941 gaacgggtgt aatccatccg gatcgctcct tcacccgacc aaaatccaca tccccgacta
     9001 attttccatc ggctaatcga ttgatcccca catcaatgac gatcgccccc tctttcacca
     9061 tgtctggggt gacgaaaagc ggtttcccga tggccgcaat cacgatgtcc gcttcccgaa
     9121 ccaccccggg gagatctttg gtccgggaat gacagatggt gacagtggca tgtcggtgca
     9181 agagcaacat agccaccggt tttcccacaa tattactccg gccaataacc acgacacgtt
     9241 tcccggcaat atcctgtccg gtcgaatcga tcatctgaat gacccctttt ggggtacagg
     9301 ggataaaccc tgggtcgccg gtcactaaac gcccgacatt gaccggatga aatccatcca
     9361 catccttctc cggagagacg gcgtgcaaaa tcgcttgact gtccatgccc gaaggaaggg
     9421 gaagctggac cagaatgcca tggatctggg gatcggcatt gagctgatga accagcttca
     9481 ataatgattc ttggggggtc gatgccggca aaagatgttc ctgaggatag agtccggcct
     9541 tttcacaggc aattctcttg ttccgcacat agacggccga tgccggatca tctcctacca
     9601 atactgcggc taatccgggc ttgattccgg tatctttttc gaaaaggcga acgtcttcag
     9661 caatgccctc tcgaatcgcc tgggctaatg ctttgccatc aatgatctgt gctggcacgt
     9721 tgactccttc aaataaaatg gtcaaaccgc tatgagcggt gatttacgat atcaggcagg
     9781 gaaaatcttc gtcaacgaaa tggcttcctc accattcctt gacaccctac aaaaacctca
     9841 cttaccatgc cccttcaggt tttcctctaa ccacatccct tgtcgctctg atcgtcctca
     9901 tcgtctcagc gaaagttcgg ttatttccat gaacccatat tgccttgctg tttctttcag
     9961 gaatgcgatc ctcaccagta tgttggtgct gggttgctgg actgctgttt atgccgagga
    10021 ggtggcgaat ttagcgttcc cgcaaagcat gattgccgat ggagggcagg tctatttcat
    10081 cgccaatgcc aacggagacc ccgggacacg cgaaaataaa ggttttatca gtaaagtgac
    10141 ccctgaggga aagatgattg atctccattt catccaaggt gggcaaaagt cggttatcct
    10201 gaactcaccc aaaggaatgg ctctggtggg cgcaactctt tatgtggctg acctggatgc
    10261 ggtccgagga tttgacaaaa acaccggaga aacgcttcac actatttcct tcgcacaatt
    10321 ccacagtcaa tcacttgccg ggctggctgc ggatcctcta ggcgggctgt atgtctctga
    10381 ttccgactcc aataccatct atcacattga tgaagccgcc cacgatcata ccgtgacggt
    10441 ctttgcccag gatgagagcc tgtcacaccc acgtggcctc accattcatc ccaggaatgg
    10501 gcatgtggtg ggggttggat gggaggatgg gaagatcttt gagattgatg aacacgggac
    10561 gattcaggag ctgttcgcca acactttttt tactggtggg ttctataatt tagacggtat
    10621 tgatttcgat aaatacggca ccatgtatgt gtctgacctc acggtaggga aagtctggcg
    10681 aatcctggcc aatcacaaaa aagaggtgat tgccgagttt ttgttgtccc cttcggggat
    10741 cggggtcgac cgtgtcaatc atgtgattat ggtcccctac ctgtatgtaa acggcgccga
    10801 aattaatggt cttgagcgtc cctctaacga taaccccaac aaaaaacccc gatcttggtc
    10861 tgattatgga ctgggctggc ttaagaaaga tgcggcaaat gagtaagtgc ctctattgtc
    10921 atcaacgtaa agggaaacgg acgtgtcccg cccttggagg aatgatttgc agccaatgtt
    10981 gcggcaccaa ccgcatcacg aatatcagtt gcccgacttc ctgcgtcttt ctcgagagca
    11041 atgatgacta ccagcaaaaa cgagtgggga accggtttga actcgaacgt cgggggttct
    11101 accgacaatt attggatctc gggggtgaac gggcgactga aatattttat gtctttgagg
    11161 ccttggctta tcgctttttc caggaccggc gtgacgcaca ggacgtcgag gtattggaag
    11221 gcctccaaag cctcaggcga agtttcagcc tgatccagat accagattcg ggaatacccg
    11281 ctttcggtga agaattacga aaggaattta aggtcttcgg cgaacgccaa cccttagaat
    11341 ctcatttggt caccaacgtc ttagaccagg ctcatatctt catccaggaa ttttccggat
    11401 cagggcttcg gtcccatcga tttttacatg gattactcgg ctatatccgg gaacgccatc
    11461 ccgatgtagc agaacaactc gcccgccaaa ccggggcggg agggcgcatc attattccca
    11521 gtggatttga ttacgcacca gacccccctt cgacagaacc cgtcagctga tcggcacgtt
    11581 tcatcaacat tcccggcttt cctctgccga gttacggcat cacatctatc gtcatttcaa
    11641 tccgctctag tggattatcc ttcaagtcgc ggggctggcc cacaattgta tcaagcactt
    11701 caaggccttt taccacttca ccaaacacgg tgtactggcc atccagatgg ggggccttat
    11761 ccaccatgag gaaaaactgg gatcccgcac tattggggtc tgccgtgcgc gccgcggaga
    11821 gaataccctt ttcgtgagca acccggttaa attctgctgg aaccgtatag cccggtccac
    11881 cggttccaaa ccggctgcgg ttggctggat ctttggtcaa aggatccccc ccttgaacca
    11941 taaatccagg aatgatccga tggaaaatgg ttccgttata aaaccctgac ttgactaatt
    12001 tgagaaagct ttccacatgc ttgggtgcta agtcgggaaa taacacggct tccatttcac
    12061 caaatttcgt tttgatcaat acgtgaggtg ccttggtatc agcattcgat ggttcttccg
    12121 cagccgcccc ggttgggaca agagaaccac ctgcggtgac aatggtggac agtccgatca
    12181 ccatcatgag ctgacgtaga gcactcgcta aagtccgtgg ctgcaacaca accctcataa
    12241 ttcctcctcc atggctaatc tgttgaattt cttgattgga tgaccaagct cgcctgaaac
    12301 atgaatctca gactgggcga gcgctttcgc tgccgcacat tgttcggctt ctttttttgt
    12361 ccgtccttct ccctgtccca taattttccc ttgaatttcc accgtgacag caaagacctt
    12421 ctgatggtca ggacctgatt cctgaacaag tcgatactgc ggacttaccc caaattgttt
    12481 atgcgtccat tcttgcagac ggcttttccc atcccacccg gctgatgaca caggactgtt
    12541 ctctaaggaa gaaaattccg tggtcaaaac tttttgaata aatgctcttg caggctccaa
    12601 ccctccatca agatacaccg ccccaatgat cgcttcaagg acattggcta atagcgatat
    12661 tttttctcga ccctttgagg cttcctcccc ccgacccaat cgtaaccact gtcccaaccg
    12721 gagacgactc gccgctttcg agagagtaga tcggctaatc aatctcgctt taatcttgga
    12781 gagttcgcct tctgtgccac ttgggaacat cgcggcaaga ctttcactca caatcaatcc
    12841 cagcaccgca tcccctaaaa attctaatcg ttcattgtgg gggctcaccg cctggggttg
    12901 agtttgagag tatgaacggt gggtgagagc ctcacacagc aaagcctgct gcttaaagac
    12961 atactccaag gattcttgaa ggtcttcaaa tgcaagagca gccacgaaac ctacaaagaa
    13021 atgctaatgt tgacaggcat gatcgttttt ctcttatttt tgaccaaaac agtgcagtat
    13081 atcatatttt ttgtggcgag attggggcaa tagatactga aactctggag aggcacatct
    13141 ctcgaaacta tccaatcaga aaggggcaat gctgcctatt cgcctttcca acgccggaag
    13201 accaaacagg cattgacacc accaaatcca aatgcattag aaatggcgac atcgacagta
    13261 caggatctgg catgagtcgg aatataatcc aagtcacatt cggggtctgg cgtctccaaa
    13321 ttgatggttg gaggaatgac gccctggtgc atggccatga tagaaaaaac cgcttcaatt
    13381 cctccggcag ctcccaatag gtggccggtc atggacttgg tagaacctac aggaatggaa
    13441 taggccagat ccccaaagac ttttttgatg acctgcgttt caatccgatc ggcaaaggtg
    13501 gatgtggcat gggcattaat atatcccaca gattctttcg cgatccccgc atcctgaatc
    13561 gcacgctcca tacaagtcac cgcacccgca ccatcgtctg gtggagcggt tatatgaaag
    13621 gcatcgctat tcatggcata gccgatcact tcggcataga tacgcgcacc acgagatttg
    13681 gcgtgctcca attcctctaa gattaagaca ccggcacctt cgccaatgac aaatccgtca
    13741 cggtctttat caaaaggccg actagcccgt ttcggttctt catttctcct ggacaacgcc
    13801 tttgccgcgg caaacccagc gaccgctgtc gggcaaatgg ttgattccgc tcctccaacc
    13861 agcatgacat cggcctctcc ccgctgaatc agtcgaaatc catctccgat acaatgattc
    13921 cctgtggcac aggccgtgac cgtacaggca ttcggaccct tggcaccaaa acgaatcgct
    13981 acctgccctg aggccaaatt aataatgacc atcgggataa aaaatggcga aacccggtcc
    14041 ggaccgcgct ccagcaacac cttatgataa tgctcaatgg ccggaagacc cccaatgcct
    14101 gctccaatat agacccccac acgatccgcc tcatccggat ttacgacaag cttggcatca
    14161 tccactgcct cctgactggc tgcaatggca aaatggataa aggtatccat ctttttaatt
    14221 tcttttttct caatgaatgt cgagggatca aagttcttga cctcccccgc aatctgcacg
    14281 gaatactggc ttgcatcaaa tcgggtaatc ggcccgatcc cggattggcc tttacatagt
    14341 ttctcccatg tattggatac accaatatcc aacggggtga ccaatcccaa tcctgtcaca
    14401 accactcgtc gtggcgtcca ttcactcata gaggaactcc gtacacatca tcgttggaaa
    14461 accccgaaac tgaatctaag attttacccc ctcgcccccc acaatcaacc atgggtgaac
    14521 cgcaatttcg cacagcgatc aatgtccttc tcagactttt tccttaataa attcaatggc
    14581 tgccgagacc gtttgaattt tttctgcatc ttcatccgga atttcgattt caaactcttc
    14641 ctctaaagcc atcaccaatt ccaccgtatc cagggaatcg gctccaagat cttccacaaa
    14701 cttggcctca gggaccacat catcttcttc cacacctaat tgttccacaa taattttttt
    14761 aacacgctct tcaactgcca ttgttggctc ctcctcattg caaagtttat cgaatgagac
    14821 acatcctgtt gcgcatgggc ttaggacgcc atatgcatcc cgccattcac atgtaacaca
    14881 tgaccgctaa tatacccggc cttctctgaa ctcagaaagc acaccgcatc ggcaacatcc
    14941 gaaggttgcc ctaaccgctt taatggaatt tgattcagta acccctcttt aatttctggc
    15001 gaaagatgtt gggtcatagc ggtatcaata aagcccggtg ccacggcatt gacggtaata
    15061 ttccgactgg catactcccg ggcaattgtt ttggtgagac caatcactcc cgctttggac
    15121 gctgcatagt tggcctggcc catgttccct atcgctccga caatggaagc aatattgact
    15181 attcgaccac ttcgttgctt gctcatggaa ggcagaacgg cctttgcaca gaaaaatgtg
    15241 ccggtcaaat tgacctgcag gacagcatgc caatcctctt ctttcatccg cattaacaaa
    15301 ccatctcgcg taatgcctgc gttattcacc aggatatcca ctcgccccca ttctttgagg
    15361 acgcgatcca tcattccctt agcctgctcc cattcgccaa cattgaccga aaccgccaac
    15421 gctttacgac cggattgttg gacaagatcc acggtttctt gacaacgttc agcatcaaga
    15481 tcggcaacca cgacatcagc cccttctctg gccaatgttg tcgcaatcgc ctgcccgatt
    15541 ccttgggcac cacccgttac cacagcaatt ttttccgtca acaacattct caaatccttc
    15601 ttgaaaattt ggcccagttt gtctttacga acagtgcgaa agaacgtgag cgtaggattt
    15661 ctgatccatt acattccacg tggtagcctc tggaacaatg cgcttgacta atccagtcaa
    15721 gactttccct ggaccgattt ccacgaaatg cgtgatgccc atcagggcca tggcctgaat
    15781 ggtttcctgc caccgtacgg gggaaggtaa ttgtcgaaca agggactcac ggacctcccc
    15841 cgctgatacc agggctttgg cttccacatt attcaccaac ggtacattta aatcagacca
    15901 ctttaaagaa tcaatatctt ttttcaatct gtcagcagcc acctgcatca atggggtatg
    15961 aaccggaacg ctgaccggaa gtggcatcac ccgcttcgct ccacgtgctt tagccaattc
    16021 tgcagcatgt tccactgccg ctttttctcc ggcaataacc gtttggcctg gagaattgaa
    16081 attggccgga gccacaacac ccacagatcc tgcttcttga cagacgcttc ggacgtcctt
    16141 ttctgacagg cctaagatcg cggcaactaa cccgcttcca ggagccacag cttccgccat
    16201 ataacgtccg cgcttctgca ccaaattaac cgcttctcga aattccagcg cgcctgccgc
    16261 cactaatgcg gtatattcgc ccaagctatg accggcaact gcagcgggaa gaaggggacc
    16321 ttcacggacc agttgaaacg cggcgagact cgtcaccagc aatgccggct gcgtatattc
    16381 agtttgattg agttgctccg acggtccctc aaaacacaat ccaccgacat catatcccaa
    16441 gatatcggcg gcttccttgt agagggcctg aaccgaaggg ctactatcaa aaagaggctt
    16501 gcccatgcca attgactgcg agccttgtcc aggaaacaaa aaaccaaaaa ctggcatcat
    16561 ccttttcgct cactctctaa aaaattccat gactcatgag cagtccccta cgtggctcct
    16621 ctcacttcct ggaactaatc aacaataaac tctccaccat ccacacaaat cacatttcca
    16681 gtgatccaat gagctttcgg atgacttaac aatcctagcg catcggcgac atcctgggtc
    16741 gtggtcagtc gacttgaagg gttttttcgt ttggctaatg tcagcatttc atcagaaccg
    16801 ggtattttac gcaaagccgg agtatccgtg acaccggcca tcagcgagtt gacggtgata
    16861 cccagaggag cgagctccag agccaattgt ctggtgtgtg actccagggc ggactttgca
    16921 gcggaaaccg ctccatacgt tcgccagact cgtgcccctc cggcactggt catggagaag
    16981 attcgactcc ctgatcccaa aaatttttgt gccactaaat cctgggtcca atacaccaga
    17041 ctgttggcca tgacatccaa cgtcatatcc atctgcgctt tattaatggc ttccgcatgg
    17101 gattcagcaa tatacggctt cagtgttcca aaagctaacg aatgtaacaa cacccgcaca
    17161 caaatcggct tccccgattc ttccgcacgt tgcttcagcg ctttcaaaat gtcggcacgc
    17221 ttttcaggat ctgctgcatt gacgttaaaa aatagaacct ctcgcccgat tgaacgaatc
    17281 tgggaagcaa tacgctccgc attcggctgt gttgattttc gatcaagatg caccccacag
    17341 atattcaacc ctaaattggc caactccaat gcggccgcct ctccgaaacc gctagaagct
    17401 ccaagaatca gcgcccaatc attttcaccc catgaggtcg ttccagacat aattcccctc
    17461 tcgttataac ctttcaaatc ttttggtttg ctcttgttgg aagcctcaga ttcttcccct
    17521 gcttttttac caataatctc attaaggaaa gagatacctg tcgacaatca cattaccaac
    17581 gaattacagt tgacgcccaa gtcagacctg caccaaatgc tgccaagagc accacactcc
    17641 ctttatgaat tctcccctcc tggacagctt gatcgagcgc caacggaatg gatgctgcag
    17701 aggtattacc aaatcgatct aaatttatca tcaacttttc tcgagccaac cccaatcgtt
    17761 ggcccacggc attcaaaatc cgcatattag cctgatgagg aatgaagaga tcgatatcgc
    17821 ctatttccaa tttattggcc tctaatgcct ctttggtggc ctcttccatt gatttgacag
    17881 caattttaaa ggtttcatta cccttcattt ttataaaaca ccgcttttct tcaagaacct
    17941 ctttggatgc cggttcccgc gatccgcctc caggcacctg aatcatatcc gaaagtccgc
    18001 cattggcatg caaatgcgtg gaaaggatcc ccgaaccttc ggtgccttga ccgactacca
    18061 ccgccccagc cccatcacca aataagacgc acgtattacg atcggtccaa tcggttatcg
    18121 ctgacatgac ttcggaacca atgaccaata cgtgccgcat cccacttttc acataggcat
    18181 ctgcaatagc caacgcataa ataaacccgc aacaggcggc agaaatatcg caagccgctg
    18241 cccgatgggc tcctaaccga tgctggataa gacaggctgt ggaaggaagg ggggaatccc
    18301 ctgtgcaagt ggctaaaagg atcaggtcga tttccgatgc cgccacatca gcggccatta
    18361 acgcacgctg ggccgcctca acagctaaat cggaacaggc ctgccctggc tcaaccacgc
    18421 ggcgctccct gatccctgtt cgttcggtaa tccaggtatc cgaagtgtca accatgcgct
    18481 ctaaatccgc attggttaag actcgtttgg gaacatacgc ccctgtccct agaatcacgc
    18541 tcttcatcct tgagacacct attttaattc acctgcaccc atgtgcaata tcgcctcgga
    18601 tttcctcaat caaaccacct tccgccaaag actgggctcg aagaatcgca tttttaatcg
    18661 ccttcgatga ggaacgacca tgacagatca tactgatacc ttcaaccccc aaaagaggag
    18721 ctccgccaaa ttcggcataa tcggtttttc gccgtaaccg cagaaggggt ccggccacaa
    18781 acagatagga aagccgacca agagctgact gggcaatttc tttcatcagc atttttttta
    18841 tcgcatccgc caacccttca gaaattttca aggctacatt gccaataaat ccatccgtca
    18901 caatgacatc ggcattccca ctatagacat cccgcccttc aacattacca atgaaattaa
    18961 tggggctatc cttcaacaat ttaaaggttt cttttgtaac ttcattccct tttgtatctt
    19021 cttctccaat acttaatagc cctacccgtg gacgatcatt tcctaggaga tgcctcccat
    19081 attcatgccc catgattcca aactggaaga gttgtttggc ggtacaatca acagtggcac
    19141 cgacatccaa catgattgcc ttgccctggc gagtcggcaa tgtcgcggcg attgcggggc
    19201 gttcaacccc ttgaatcaat cccaaaagaa aaaacgctga caccatggtg gcaccggtat
    19261 ttcctgcaga caccaacgca tcggcctgtt tatttttaac taattctgtg gctttccata
    19321 tggaagaatc tcgttttttt ctggcaatca acgctggaga ctcatgcata tccacgactt
    19381 gtggggcatg aaaaatctca atgccggctt tggtaccgcc gagtttgtcc agatgagatt
    19441 ggagagatgg ttcgtctccg accaggataa tcgagagaga ggattccctt gtagcctgta
    19501 tggctccttc gacattggga gataatccat gatccccccc catcgcgtcc acggctattt
    19561 tcatgggttt ggtg
//
LOCUS       DAORAQ010000042        19521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_502_length_19521_cov_69.060105, whole genome shotgun sequence.
ACCESSION   DAORAQ010000042 DAORAQ010000000
VERSION     DAORAQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 19521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19521
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_502_length_19521_cov_69.060105"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKM72_05955"
     CDS             <1..237
                     /locus_tag="PKM72_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60363.1"
                     /translation="KLHARVVEGMVAAVEILAEINVVAGSNQNTNTFQKRERSMRPLP
                     FFVKIQQLQGMVNISQTGINWTCGECSVQSGYIA"
     gene            487..2619
                     /locus_tag="PKM72_05960"
     CDS             487..2619
                     /locus_tag="PKM72_05960"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNP60364.1"
                     /translation="MTSFPPKIQPVSVLPYAFAIFLSAFLLFQVEPIIARYILPWFGG
                     TPAVWTTCMLFFQVFLLGGYGYAHLLASHLSPRHQALVHLGLVVCSLVFLPITPDDVW
                     KPDGTQDPMLAIILLLMVTIGVPFLLISASGPLLQHWFNRVHPTVAPYRLFALSNLGS
                     LLGLVSYPFFIEPQLGLRMQTLFWSAGYGLYAVMCTWGALPLFRFPLRSESSENPEYR
                     QDKKPRLPESFLILSLAACGSVVLLASTNQICRDIAVIPFLWVLPLGLYLISFILCFD
                     HPRWYDRRIWVPVLLVLLSAVVYLLHQEYAETEVIVYIQIFIYSAVLFACCMVCHGEL
                     VRLRPPARFLTSFYLMVALGGALGGVFVNLVAPVLFKGYWEFHFGLVATMMLLAICLF
                     RTRDPLRSPRILWSERVFWVGGIATLAGFLALHIQEEQASTILTTRNFYGVLRVKETE
                     RGTESASRFLYHGRINHGRQFLTPRRHMYPTAYYGPFSGISLAINRHPRQLRLNTLED
                     RKAQGGLRVGNIGLGVGTTAVYSRSGDIYRFYEINPDVDRMAREYFTYLKNAQGAQQV
                     VLGDGRISLERELGNKHRQQFDILAVDAFSGDGIPVHLLTREAFALYWEHLRPDGILA
                     LHITNLHFDFSPVVRALARESGKQALWIKDMADHGKGNSYSDWVLVTNNQAFLDDFFV
                     NIRIVPWRSLEEIIWTDDYSNLFQVVAP"
     gene            complement(2821..3192)
                     /locus_tag="PKM72_05965"
     CDS             complement(2821..3192)
                     /locus_tag="PKM72_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60365.1"
                     /translation="MKRILLVDDHEMARKAMKLFLEHHGYACDEAEHGAAALVRLDQG
                     PSVDLIVSDNFMPIMTGMDFLLHTKANPQFSSIPFILYSGNITDEMSQRANEAGALAV
                     LSKPYNFLNFVTLVNKTLETP"
     gene            4183..4713
                     /locus_tag="PKM72_05970"
     CDS             4183..4713
                     /locus_tag="PKM72_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNP60366.1"
                     /translation="MDMMQLQVAQMFDQHRQEVKRFLISRVSCEATAADLTQETFLRL
                     SQLSDLQSIRNIRSYLFRIAANLATDHLRTQIRWKMASSEEEPLLLEKADTSSTPESV
                     LVAKEELRIVLQAIQELSPLCREIFLLNRYEGFAHREIANRLNICMSTVEKNIARALN
                     QCRRRLNEAAESSQKL"
     gene            5019..6044
                     /locus_tag="PKM72_05975"
     CDS             5019..6044
                     /locus_tag="PKM72_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNP60367.1"
                     /translation="MSDADCPDSEQERLEAEAVTWMVRLTSGETTDADRRAANLWCRK
                     SVAHQRAMEKAFRIWNGMEGLRDSLIIPDRSGEVRGQTQRTIRHPLFPSAYFYRQDWW
                     VWKVIVAAIAVLALSYTFQSDFLIDWRADYSTGTGEQATWEFIDGSIVQLNTHTALNI
                     AYSQNMRRVDLLQGEAAFRVAKDPSRPFIVQADQGTIRAVGTEFFVYKLDRSVLVTVV
                     EGVVDVSVPLGHERPSSSARLKSGQSVQFGTNSGLSEVKDVDLGIATAWQRGRLIFEA
                     APLTEVVEEINRYRSGHIVIVNDTLREHLVSGVFDLDSLDSAVTTIERTLPVASLHLT
                     DRLIVLQ"
     gene            complement(6069..6317)
                     /locus_tag="PKM72_05980"
     CDS             complement(6069..6317)
                     /locus_tag="PKM72_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60368.1"
                     /translation="MPLTLQVAGQARLKKKAPLLFRKFDFKVSTYDKEGQPPLNMPTS
                     QDGSAEETQTDKIQKFCRSDPSPQKANKCLFSARRFVE"
     gene            6387..8951
                     /locus_tag="PKM72_05985"
     CDS             6387..8951
                     /locus_tag="PKM72_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNP60369.1"
                     /translation="MSKTSVGAQRQKAKEFRRKRVSGWLMGLTLVILQLLGNLTNSAS
                     AQESIRFDIPSQALNTALTAFAEQSSWQLFYSTEIAEGLQNKALSGAHEPESALKHLL
                     SGTGLEYRITGPQIVRISKGGNSAVLPPVPLADTSVQQSSQSVNGQTSQPRTVKVPEV
                     VVKDVRDRPYTTEDVSSASRIPVPVQEMPRSVETVTKEVIEDQKVIRMNEALRNASGT
                     YQSSTQGGQGGTFIIRGFASDLNVFKNGFRDDSTFSSRAPRDIINLESIEVVKGPPSY
                     LYGRSDPGGVINQITKAPLKNAFYSGEMIGGSYELYRPTVELGGPLNQSKTLTYRFNG
                     MFESAESYREGVKSKRAFLAPVFGWDISSRTTFRLEGEYLYNKAPIDRGLVAIGDGVA
                     PISIKSFLGDPTKKDENNSGKVTATVLHEFNDMFTWRTAYRGAWTSSRYDALESWFLV
                     GPESDGILDLARFKLPTNVSSNYMQNEVHGKFSTGSIKHKTLFGIELGRESSSAKAYS
                     DFGGDTTTPGGFSYINIFDTSDKQFVDIPLDEFSHTKQTNYIFGLYFGDQIDLLENLH
                     VHGGGRFDIFEQKLTNRPTDFDPDTNKDKQTKKRFSPSVGVTYQPWKPMAIYANYTES
                     FVPQAIGSRGIDGELFKPERGKAWEGGFKFQFFDNKLRTTMAVFQIDKTNVLTADPAN
                     GFAFSQATGKQRSRGFELDVAGTILPGWEIIANYAYTDTRVRKDVMFKEGSRVSNVPL
                     NQGSIWTTYFFQEGVVKGFGAGIGIYAQGEKNGVFECEDPANCQAPFEMKGYVRMDAA
                     LYYRKPNFSQGTNLLAAVNFTNLLDQRYMTGSQNFREIVYTGAPLTVIGSIKFEFF"
     gene            complement(9462..>9658)
                     /locus_tag="PKM72_05990"
     CDS             complement(9462..>9658)
                     /locus_tag="PKM72_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60370.1"
                     /translation="HLSCKLQRGRQFPSSENEHKAKSLRLKFSGHYNTERTAHGDPFS
                     IKFGKIYCDQIPAGQRPENI"
     assembly_gap    9659..9758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9933..10994
                     /locus_tag="PKM72_05995"
     CDS             9933..10994
                     /locus_tag="PKM72_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60371.1"
                     /translation="MNARFILFTARSGQVSGTFLLTVFLVLCCWGAIALADTPGPGAT
                     EKDDKFRRDREAILAMAGNYRVTFDFRETVSFVEGYQLKKPYDVDGYEIVRVIRDDGE
                     FISLQHILVVDGIWEDQMPIKHWRQDWIYEPTQQFEYVGRHVWQIRQLDEAERRGNWA
                     QLVYQVDDSPRYAGVAAWTHRHGESAWTSPPTWRPLPRRERTKRDDYDVLISVNRQAL
                     TPNGWVHEQDSSKIILRTDPKILAREMGVNTYTSSNAFKSQVAEDYWNETKEFWAEVR
                     DEWSKIQQDFEAFSVESHGQSGKLYNQILRLAKDAREGSLAMKTAARRARILIRDSTT
                     NEVSVRNLRAEVQGQKANN"
     gene            complement(11170..11511)
                     /locus_tag="PKM72_06000"
     CDS             complement(11170..11511)
                     /locus_tag="PKM72_06000"
                     /inference="COORDINATES: protein motif:HMM:NF023232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HNP60372.1"
                     /translation="MDILVFLLSYAGLFTLCLSMPKHFQVMLGKPYLPSSPSMLILKI
                     VGWLILGLSFAMAVYTAGWSFGPVRWVGYLAIGGLTLIFGLPFAPRLVLLLGILTCFG
                     GPLAVWVFLLD"
     gene            complement(11535..13085)
                     /locus_tag="PKM72_06005"
     CDS             complement(11535..13085)
                     /locus_tag="PKM72_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNP60373.1"
                     /translation="MKESFTQCMDWLHTWAGLLFGWLLFAIFFTGTLTVFDQEITYWM
                     QPELHHVIPSPLNVDAVMRTLEQFAPQAERWSIELPSERTPVSTIRWSQDGTSVSRMI
                     NAKTGQPITVRNTHGGEFFYRFHYQLLLDQAGTWIVGAAAMAMLAALVTGIVIHHRIF
                     KDFFTFRPGASPHRSWLDAHNITSVLALPFHIMITFSGLVIFWSIYMPAGLQVHYDGE
                     MDGFWAEAESFIEPERANQPAPLVSLRDLEREARPYWQGGQTRSIVVSHPGDRHALVH
                     VNRQSNDRLSLYSDRVSFDGASGALRTVWINSKPAYMTQSVLSGLHFVQFGGATIRWL
                     YFGMGLAASAMIATGLVLWVVKRQGRHTTTDAVGYRLVEGVNVAAIAGLLVATATFFW
                     ANRLLPADLPARELRELQIFFMAWSLCLGHALIKKLFQLRPSLRIWKTQLSVAALFFI
                     LLPVVNVITTEHHLFMTVPKGQWEVAAVDLTALIGGLGLGWTARRLDQPARTSSRPRP
                     SHPVASGG"
     gene            complement(13132..13425)
                     /locus_tag="PKM72_06010"
     CDS             complement(13132..13425)
                     /locus_tag="PKM72_06010"
                     /inference="COORDINATES: protein motif:HMM:NF023783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3649 domain-containing protein"
                     /protein_id="HNP60374.1"
                     /translation="MLSKARRTKSSNTWITTSSRVAAAVFGGYALAYACTAWLSMVLP
                     LAKSEAVLTASMMSFLIYTGAILCAFATATPMRAWVVLLIPTLLLGGMTLLMT"
     gene            complement(13836..14744)
                     /locus_tag="PKM72_06015"
     CDS             complement(13836..14744)
                     /locus_tag="PKM72_06015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNP60375.1"
                     /translation="MQTDLKNMLMASVNDQGVLSKESSQDLVPWWSFTKTLIAACALR
                     LVEQGHVNLDNSLEGKPYTLRQLLQHRAGIANYGGLRAYHEAVSRGEEPWTTEELLAR
                     MNVENLRFNPGQRFMYSNIGYFYVCRLMERITGEPLSRCLHRLVFGPLNLGSARVVET
                     REDMELSTFKTDHGYHPGWVYHGLIMGSVAEAALGLYGLLNGKLLRTHSLNEMLQCYP
                     VGGEIPGRPWKTTGYGLGFMIGQIGREGMAQSTRAIGHSAAGSGSVGAVYHFPDTSSK
                     RTVAVFGPFKDEGITEAAALQIAMGI"
     gene            complement(14946..16121)
                     /locus_tag="PKM72_06020"
     CDS             complement(14946..16121)
                     /locus_tag="PKM72_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP60376.1"
                     /translation="MTRILLPIGVLSMGLLTAFLLLTNRSQPEAESLQPEPPRVEVIE
                     VQPQSVRVTVTSQGVAMPRTEIDLVTEVAGKVIHVHPAFAAGGSFYRGDVLVAVDPRD
                     YQFARIEAQSRLAEAQRLLAQEEAQVEQALKEWQVLGKGEATALALRTPQLREIRAKV
                     AAAKADGAKAKLQQIRCVLRAPFDGRIRAKHVDMGQYIVPGEKVARLYATDSLEVRLP
                     VSMEQLAFIDIPWISEKNGSTHTAPSVTLHTTIGGIMGRWVGYIIRTEGALEPSSGQL
                     SLVARVSQPKSDQHHGRPLLPGTFLQAEIEGREYHQVFVLPPAVLNTRTEQVLIVDDT
                     NRLKLQQLDVLKSETDRLVVRGGLQAGDQVVVSGIDVPIEGMQVTIATPSPIVAPIP"
     gene            complement(16118..17509)
                     /locus_tag="PKM72_06025"
     CDS             complement(16118..17509)
                     /locus_tag="PKM72_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP60377.1"
                     /translation="MSETGKPLIMSAGVCLILLSGCLSTPERDGEVIMSQVPEQWSKI
                     TDRTVEHVTDSWVDTFHLPILTAMVREALEFNHDLKAAAARVEIARANVRIAGAPRLP
                     QVNFSPDFRHGRFGTFDSDVANENYSFMEVVFNLSWELDIWGRIRASQEAAALDAMAV
                     ETDLQGAALSLAARTAQSWFELLEAGLQEKVASQSVADRRMLVDLVRGRFTRGLTPGL
                     DLGLALTDLANAEAQLADTQNQVQLTARRLEVLLGRYPAGELNAQLPLPALPSTLTAG
                     VPSELLSRRPDIAAAMERLRAEDQRFISANLALLPRITLTGTGGTRSNSLKELTQPGS
                     LAWNVGAGLLQPIFAGDQLYGEIARNRAAVTEAMENFQQTALEAFREVEQALAAEEQL
                     SAQEQALSEAVKHAQANQQMAVYAYRHGFTTILTLLDSFRGTLMAQSAHLTVKRRLLN
                     NRIDIYLALGGAI"
     gene            complement(17821..>19521)
                     /locus_tag="PKM72_06030"
     CDS             complement(17821..>19521)
                     /locus_tag="PKM72_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP60378.1"
                     /translation="LVEALCILPAHLTTPVPVSSRWMATLRQRVTRGLEAFIHCAYLP
                     LLDRSCRQPYLIIAFCASAIMITAALVSGGHVRLAIQADVPKDTITLYLDVPPGAPFT
                     TVQRLSTQIEEAYKELRHSLETRQPPEEPSPVSGIESMIFDNGSGFWVELAPGARQRF
                     SVETIAREWRTHIGDIGKAKLDFLYRQGDNYYDLEWAVSATDSETMTAALATLTEHLS
                     SLPGVFDVMHSQIKGKPQLSLELRPEAERLGLRLEDVATQVRQAYLGEEVQRFQRQRE
                     QVKVVVRLPLNERRDFNDFRQLPILLPTGDQAPLEVLATWRWLPQTESITRQNRQQLV
                     WVRARLNPELADANAIYATMQTGFFENLKQRFPGLNITVGHTRQDQMAAFETLGRNAL
                     FAVGIIYALLAVSFRSFRYPVLILLAVPMAWVGGVLAHFVLGLPLSMESLVGMIGASG
                     VIINDGLVLLNYVQRRKRKETALNQQSLIRQACEIRFRPIVLTFLTTLVGLVPMLFET
                     SAQAQFLVPMAVALAAGLFGGLVATLLLIPATTLILERSLSPTHPATLSSHGDAPEYS
                     "
BASE COUNT         4684 a         4775 c         5192 g         4770 t
ORIGIN      
        1 aagctccacg cacgggtggt ggagggtatg gtggcggcgg tggagatttt ggcggagata
       61 aacgtcgtag ccggttctaa ccagaacacc aatacgtttc agaaaaggga gaggtcaatg
      121 agacctctcc ctttttttgt aaaaattcag cagttgcaag gtatggtcaa tataagtcaa
      181 acgggcatta attggacctg tggagagtgc agcgtccaga gcggatatat agcctaacat
      241 cttgccattt gttaaacacg acagggcttg gcggagcacg cagtcgtgtt attgttttcc
      301 gactcagggc acatcggatt gattctcaac gcgttaaagg acggaccgag tgcatggatc
      361 tgaactgaat gaagagttga tacataccag ttagagccag ggtagtcata atcccgccaa
      421 tgaccttgca aaaacggctg cgcctgcgaa caatgtcaca tcgccacagt ctgtcaaatt
      481 agggtcatga cttccttccc accaaaaatt caaccggtgt ctgtcctccc gtacgccttt
      541 gcgatctttc tgagtgcctt tctgctgttt caagtagagc ccatcatcgc ccgctatatt
      601 cttccctggt ttggtggcac cccggcggtc tggaccacgt gcatgctctt cttccaggtc
      661 tttcttcttg gtgggtatgg ctatgcccat cttctcgcaa gccacctctc ccctcggcat
      721 caggcgttgg ttcacctcgg tctggtggta tgttccctgg tctttctccc tatcacccca
      781 gatgatgtat ggaagccgga tgggacacag gaccccatgc tggcgattat tcttctcctc
      841 atggtgacga ttggggtgcc gtttctcctc atctcggctt ctggccctct cctgcaacat
      901 tggttcaacc gagtccatcc cactgtggca ccctatcggc ttttcgcctt atccaatctg
      961 ggctctctct tgggattggt ctcctatccg ttttttatcg agccgcaact ggggttgcga
     1021 atgcagaccc tcttctggtc ggccgggtac ggactgtacg cggtcatgtg tacctggggg
     1081 gccttaccgc tgtttcgatt ccccctgaga agcgaatcct ccgaaaatcc tgagtatcga
     1141 caagacaaaa agccgcgtct gccggagagt ttcctgatcc tctccctagc agcttgcgga
     1201 tcagtggtcc tcctagcaag cacgaatcag atttgtcggg atatcgcggt catcccgttt
     1261 ctttgggttc tcccgctggg tctctacctt atctccttca ttctgtgttt tgatcacccg
     1321 cgatggtacg accgacggat ctgggtgccc gtgttgctgg tattgctctc cgcggtcgtc
     1381 tacctgctgc accaggaata tgcggagacg gaggtcattg tctatatcca aattttcatc
     1441 tattcggccg tcctctttgc ctgctgcatg gtgtgccatg gcgaactggt tcgcttgagg
     1501 cctcccgcca gatttctcac atccttttat ctcatggtcg cgttgggagg agccttgggg
     1561 ggggtgtttg tcaacctggt cgcacctgtt ctctttaaag gatactggga atttcatttt
     1621 gggctggtgg ccacgatgat gctcctggca atctgcctgt ttcggacgag ggatccgctc
     1681 agatcaccac gcattctctg gtcagaaagg gttttttggg ttgggggaat tgccacgctt
     1741 gcaggttttc tggccctgca tattcaggag gagcaagcga gcaccatcct gacaacgcgt
     1801 aatttttatg gtgttctccg agtgaaggag acggagagag ggacggagtc cgccagccgt
     1861 ttcctctatc atggtcgcat caaccatggc aggcaattcc taactccccg tcggcacatg
     1921 tacccgactg cctattacgg gccgttcagt ggaatcagct tggccatcaa ccgtcatccc
     1981 cgacaactga ggttgaacac actcgaggac aggaaagcgc aagggggctt gcgcgtcggg
     2041 aacatcgggt tgggggtagg caccaccgca gtgtattccc ggtctggtga tatctatcga
     2101 ttttatgaaa tcaatcctga cgtggatcga atggccaggg agtacttcac gtatctcaaa
     2161 aacgcccagg gagcccagca ggttgtcctt ggagatggac gaatttctct cgagcgagaa
     2221 ttggggaaca agcatcgaca gcagttcgac atcttagcgg tagatgcctt cagtggcgac
     2281 ggcattcctg tgcatctcct tacgagagag gcgtttgctc tgtactggga gcatctacga
     2341 cctgacggga tcctcgccct ccatatcaca aaccttcact ttgacttcag ccccgtagtc
     2401 cgggccctgg cccgagagtc aggtaagcag gccctgtgga tcaaggacat ggctgatcat
     2461 gggaaaggga atagctatag tgattgggtt ctggtcacga acaaccaggc atttttggat
     2521 gacttcttcg tcaacatccg gatcgtaccc tggcgatctc tcgaagaaat tatctggaca
     2581 gacgactaca gcaatctctt tcaagtggtg gccccatgac ctgtcctctg gtgtgattgc
     2641 ctgcgtggtt cgtgaaggcg ggtgtctgac catgtctcag agcatgcttc actgattctt
     2701 ccgcaaagtc gttgcaggtg cgaaaggaaa gcttctctag gctacctagt acattaggtc
     2761 caatgtgagc tatcgcaatc ttttggagat gatggacatc gggagtgccc ccttgatgat
     2821 ttacggtgtt tcgagcgttt tattgaccaa cgttacaaaa tttaaaaaat tgtatggttt
     2881 gctcagcacg gctagggctc ctgcctcatt tgcccgttgg gacatttcgt cggtgatgtt
     2941 tcccgaatac agaataaaag ggatggagct gaactgagga ttggcttttg tgtggaggag
     3001 aaagtccatc cctgtcatga tgggcatgaa gttatcggaa acgatgagat ccacactcgg
     3061 tccttgatcc aacctgacca gagcggcggc tccatgctcg gcttcatcac aggcataccc
     3121 atggtgctca agaaaaagct tcatggcttt cctggccatt tcatgatcat ctacaaggag
     3181 aatgcgtttc atgatggaaa acctattcca gaaagagaaa ggttaggtgc gttcaatcct
     3241 tagtgaagga aacaccagca gctcccagat cgaaacgaat tttacaggaa ccgatctact
     3301 gtattctctt ctttcaaaaa tttcaaagga ggcgtgagga gggggtgata gctttttccc
     3361 acagagactt ccccagagcg gagcgatact gtcccaaccc ggccgccaag tgtgttgaaa
     3421 ttttgaggat ttcagctcaa cttagaaatg gtatatcggt atctacctag gcggctcttt
     3481 tgtcgatcct caaaaaaggc catggcgtgg ttgaaaaatt gatactcaga ctttcctgtc
     3541 tttcaaaaga gtgaaaagta cctaatcaca gctgcgatgc aaaagggtaa tccaggtcga
     3601 ctgtatggcg gtgtggtaac gaaggacagc atgccccgtg gccacatgaa gattgcctct
     3661 tcggcatttt ttaacaaaag cggaatgaaa ataatcgaaa tccttaagat gaattaaccg
     3721 gaagcaacta aagccaggtc ctggttaggg aggagagtag tggggaattt tagtttgacg
     3781 aaaattcata tgaactgagg taaccctgtt ttcatggttt cagagggatt gacgaacagg
     3841 cttagtttat attaataata acaatcaata tcaattttta ggtttacagg accattcttc
     3901 gtatcgccat ggctacttct ttctattgaa aaatttaaat gacatacaac accagcggcg
     3961 ctggttcgaa tgagaggcta gagatccgag agaccaaagc ccaatgatgc aactgcacat
     4021 tgggcttttt taatggataa aacaagaaga ttccacgtgt aacggatttc attttatggg
     4081 attgcgaaaa tttgtggata agcgctacca aggggtgaca gtggttttgg ggttgaaaaa
     4141 gtgcaccaaa ttgaaggact ttctgccata aataggaaga tcatggatat gatgcaatta
     4201 caggtggctc aaatgtttga tcagcatagg caagaggtga agcgttttct catcagtcgt
     4261 gtctcttgtg aggcgactgc tgcggatcta acccaggaaa ccttccttcg cttatctcaa
     4321 ttatcagatc ttcagtcgat tcgtaacatt cgatcctacc tttttcgaat tgctgccaat
     4381 ttggcgacgg atcatctccg aacgcaaata cgatggaaaa tggcatcctc cgaagaggag
     4441 cctctcctgc ttgagaaagc ggatacctca tccacgccgg aatcggttct ggtagcaaaa
     4501 gaagaattga ggattgtcct tcaagctatt caggaattgt cgccgttgtg tcgggaaatt
     4561 tttttgttga atcgatatga aggttttgcg catcgtgaaa tcgcgaatcg gttgaatatc
     4621 tgtatgagta ctgtcgaaaa aaatatcgcc cgtgcactca atcaatgccg tcgccgactg
     4681 aacgaggcag cggaatcctc ccaaaagctc tagccgtttt ttcagaacct ctccctttta
     4741 acatcagggg ctactgaatt ccctcgtgtc cctggtattt atcgggccac ccgcataatt
     4801 tttctaataa actgtagggt ctgattggat ctggcgcagt cctcctcccc ttcattcaat
     4861 ctccctgtgt gggaaaacaa aaaaagtaag atggagtcat tcatgtgtgg accatccggt
     4921 ttttgtcgat tgaatcgttc ttatagtgaa tttcttgaac ggcacagggt attatccgat
     4981 gcacattatt caacaggttg ggagggggga actgtcagat gtcggacgct gattgcccgg
     5041 acagcgaaca agagcgtctg gaagccgaag ctgtgacgtg gatggtccgc ctcacatcag
     5101 gggagacgac tgatgcagac cgtcgtgcag cgaacttgtg gtgccggaaa agtgtggcgc
     5161 atcagcgagc catggagaag gcgttccgaa tttggaatgg tatggaagga ttgcgagact
     5221 cactaatcat tccggacaga tctggggaag tccgcggaca gactcagcgg acaattcggc
     5281 accctctttt cccatcagca tatttttaca ggcaagactg gtgggtttgg aaagtgatcg
     5341 tcgccgctat tgcggtattg gctctttcct atacttttca atccgatttc ctcatcgact
     5401 ggagggcgga ttattccacc ggaacgggag agcaagccac atgggaattt atagacggca
     5461 gtattgtcca attaaacaca catactgcgt tgaatattgc ctattcccag aatatgaggc
     5521 gtgtggatct gctgcaagga gaagcagcgt ttcgcgtcgc caaggatccc tcacggccat
     5581 ttattgtgca agccgaccaa ggaacgattc gcgcggtggg gactgaattc ttcgtctata
     5641 agttggatcg ctctgtcctt gtgacggtgg tagaaggtgt cgtcgatgtg tcggttccgc
     5701 tagggcacga aaggcccagc tcctccgcac gtctgaaatc cggtcaaagt gttcaatttg
     5761 ggaccaactc cggattgagt gaagtgaaag acgtagattt aggcatagcc acagcctggc
     5821 agcgtggaag actcattttt gaggctgccc ccttgactga ggtggtggaa gagatcaatc
     5881 gttaccggtc cggacacatc gttattgtca acgacaccct tcgagaacat ttggtcagcg
     5941 gtgtattcga tcttgatagt ttagactccg ctgtgaccac gattgagcgg accttgcctg
     6001 ttgcctcgct gcatctcact gatcgattaa ttgtcctcca ataagattcc ttactcccct
     6061 atcccctttc actcaacaaa ccttcttgcg gaaaaaagac atttgttcgc tttttgcgga
     6121 gagggatcgc tcctgcaaaa tttttggatt ttgtctgtct gggtttcttc tgctgaaccg
     6181 tcttgtgaag taggcatgtt caagggaggc tgaccttctt tgtcgtaagt gctcaccttg
     6241 aaatcgaatt tcctaaagag aagcggtgct ttcttcttga gccgcgcttg gccagcaacc
     6301 tgaagagtta gcggcatggc ccatcatttt tttctttata tataccatac ctcattcaca
     6361 tgatgattta ggaaggaagt gaaacgatgt caaaaactag tgtaggagca caaagacaga
     6421 aagcaaagga gttcaggcga aagcgagtgt caggatggtt gatggggctg accttggtga
     6481 tcctccaact cctggggaat ttgacgaact ctgcatccgc gcaagagtca attcggttcg
     6541 acattccttc gcaagctctg aataccgcac tgacagcctt tgcggaacaa agttcatggc
     6601 aattatttta tagcacggag atcgctgaag gccttcagaa caaggccttg tcgggtgcgc
     6661 atgaaccgga atccgccctg aagcatttac tgtcaggtac aggcctcgaa tatcgcataa
     6721 ctggtcctca gatcgtgagg attagtaaag gggggaattc tgcagtcctg ccccctgtgc
     6781 ctctggcgga tacttccgta caacaatcct cccaaagcgt gaatggccaa acatcccagc
     6841 caaggacagt gaaggtgccg gaggttgtgg tgaaggatgt tcgtgatcgt ccgtacacca
     6901 ccgaagatgt gtcaagtgcc tccagaattc ctgttcccgt ccaggagatg ccccggtcgg
     6961 ttgaaacggt gacaaaggag gtgattgaag accagaaggt cattcgtatg aacgaggcgc
     7021 ttcgaaatgc gagtgggacc tatcaatcca gtacacaagg cggtcagggc ggcaccttca
     7081 taatacgggg ctttgcgtcc gatctgaatg tgttcaagaa cggctttcgg gacgacagca
     7141 catttagttc gcgagctcca cgcgacatta tcaaccttga gagcattgaa gtggtaaagg
     7201 ggccgccgtc ttatctttac gggcgttccg atccgggcgg tgtgatcaat caaattacga
     7261 aagccccttt gaagaacgct ttttattcgg gagaaatgat tggcggcagt tatgaactct
     7321 atcgcccgac ggtagagctc ggagggccgt tgaaccaaag taagacgttg acctatcgat
     7381 tcaatggcat gttcgaatcg gccgaaagtt atcgggaggg ggtcaagtca aaacgcgctt
     7441 ttctcgcccc ggttttcggg tgggacatca gctctcggac gacgttccgg cttgagggtg
     7501 aatatctcta taataaggcc cctatcgatc gagggttggt ggccatcggt gacggagtcg
     7561 cgccgatctc tatcaagagt ttcctcggcg acccgacaaa aaaagatgag aataatagcg
     7621 gaaaggttac cgcgacagtt ctgcacgaat tcaatgatat gttcacatgg cgcactgcgt
     7681 atagaggggc ttggaccagc agccgatatg acgctctgga gtcctggttt ttggtcggtc
     7741 ctgaaagtga tggaattctc gatctggcgc gatttaaatt accgacgaac gtttcaagca
     7801 actacatgca aaacgaagtc cacggcaaat tctcaaccgg ttcgattaag cataaaaccc
     7861 tttttggcat cgaactgggt cgagagagtt cctcggcaaa agcctactcg gatttcggtg
     7921 gtgatacaac cacaccggga ggattcagct acattaatat cttcgataca tctgacaagc
     7981 agttcgtaga cattcctctt gatgaattca gccataccaa acaaaccaac tatattttcg
     8041 gtctttattt cggcgatcaa atcgacctcc tggaaaacct ccatgtccat ggaggcggtc
     8101 ggttcgatat ctttgaacag aaactcacca accgcccaac ggattttgat cccgacacga
     8161 acaaggataa gcaaacaaaa aagagattca gtccgtcggt gggtgttacg tatcaaccgt
     8221 ggaagcctat ggccatctat gccaattaca cagagtcctt cgtgcctcaa gcaatcggat
     8281 cgagagggat cgacggagaa ctatttaagc ccgagcgtgg gaaagcatgg gaagggggtt
     8341 tcaaattcca gttttttgac aacaaattgc ggactaccat ggctgtgttt cagatcgaca
     8401 aaacgaatgt gttgacggcc gaccccgcca acgggttcgc gttttctcag gcaaccggta
     8461 agcagcgcag cagagggttc gaattggacg tggcggggac gatattgcct gggtgggaga
     8521 tcattgccaa ctatgcctat accgatacgc gtgtccgcaa agatgtcatg tttaaagaag
     8581 gaagccgagt ctccaacgtc cctttaaacc agggcagcat atggacaacc tactttttcc
     8641 aagaaggcgt ggtgaagggc ttcggggccg gaatcggtat atatgcgcaa ggggaaaaaa
     8701 atggtgtctt cgaatgcgaa gatccggcca actgccaagc gccttttgag atgaaaggct
     8761 atgtcagaat ggatgcggca ttgtattacc gtaagccgaa tttctcccaa ggaactaacc
     8821 tgctggctgc ggtaaatttt acgaaccttc tggatcaacg gtacatgacc gggagtcaga
     8881 attttaggga gatcgtctat accggtgcac ctttaaccgt gattggctct attaaattcg
     8941 agtttttctg agaatttgga tattagataa gaattttcga cgctcaaatt tccgaaaacg
     9001 tgggatcctg agaaatgggc gggggtctga cataatctag gcaatggtct tcatgaagag
     9061 ggctgacctg atttggcacc tgatgcttga ggaggttttt tcggtttcgt gcaacgggtg
     9121 tccgatcctt tcgtacgagt aagcacagaa atggcgagat tccgtgggcc aatttacaga
     9181 aaggcctaaa actgattgtg agatttcgat caacgtcgaa aggatcttca tgacaaaatg
     9241 ggtgcaagga cccacacaac cacactccga cgcttctttg aaaacgagtt catggaatcg
     9301 atccgcattg atggcaaaca attcttgtgg acttgagatg atgagaagga attcgaactg
     9361 catggagaag tttcgacgat gaaagtgcct gaacctcatt gtccaggaat tgtctggaag
     9421 tttggaaatc ggtgcattga tgcgttagtg cttctagcga attagatgtt ctctggcctt
     9481 tgaccggcgg gaatttggtc gcagtagatc tttccaaatt taatggaaaa tggatcacca
     9541 tgtgctgtcc gttccgtgtt gtagtggccc gaaaatttga gtcgtaacga tttcgcctta
     9601 tgttcatttt ctgaacttgg gaattgtcga ccccgttgta atttgcagga aagatggtnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc attttctgaa cttgggaatt
     9781 gtcgaccccg ttgtaatttg caggaaagat ggtgggagtg tatggcttgt tcttttcctt
     9841 ctcaacggaa gaaatctacc ttcggatcgt ttatgccgga cgaggtcctc tttggaagaa
     9901 ccttctggaa taaagtagtg gaggaagggc caatgaatgc aagatttatc ctgttcacgg
     9961 ccagatcggg acaggtgtcc ggaacgtttc tgctgaccgt cttcctcgtg ttgtgttgtt
    10021 ggggcgctat tgctctggca gatactccgg gaccgggagc gacggagaaa gatgacaaat
    10081 tccggcgcga tcgcgaagcc atccttgcga tggcgggtaa ttaccgtgtg acgttcgatt
    10141 tcagagagac ggtttcgttc gtcgagggat atcagctcaa aaagccgtat gatgtcgatg
    10201 gctatgaaat cgttcgtgtg atccgcgacg acggtgaatt tattagcctt caacacattc
    10261 tcgtcgttga tggaatttgg gaagaccaaa tgcccatcaa gcattggcgg caggattgga
    10321 tctacgaacc gacacaacag tttgagtatg tcggccgcca tgtctggcaa atccgccagc
    10381 tggatgaggc cgaacgtcga gggaactggg cccagctcgt gtaccaggtt gatgatagcc
    10441 ccaggtatgc cggagttgcg gcatggacac accgccacgg ggagtccgca tggacatccc
    10501 ctccgacctg gcgccccctt ccacggcgtg aacggaccaa gcgggacgat tatgatgttc
    10561 tgatttccgt gaaccgtcag gcgttaactc cgaacgggtg ggtccatgaa caagacagtt
    10621 cgaaaatcat cctacggacc gacccgaaaa ttctggctag agagatgggt gtgaatacct
    10681 atacgtcgtc gaatgcgttt aaaagtcagg ttgccgaaga ctattggaac gaaactaagg
    10741 agttttgggc ggaggtcagg gatgaatgga gcaagattca acaggatttt gaggccttca
    10801 gcgtggaatc tcatggacaa tccggaaaac tctataatca aattctgcgt ttggccaagg
    10861 acgcgaggga aggaagtctg gccatgaaga ctgccgcgag gcgcgcacgg atcctcattc
    10921 gcgattcgac gacgaacgag gtctctgtgc gcaatttgcg ggcggaggtt cagggtcaga
    10981 aagcaaacaa ctaaccctga tcgagggcgc gtgattccct cgtggctgtg gtcattctca
    11041 acatgcctgg taaatggata tagccgggat ttttatcaga tattttccag gtttatcttc
    11101 cggtcggagc gttcccttca aggcagtggc tatgccgatt atttcggagt gactggaaag
    11161 acctttccgt taatctaaca aaaaaaccca gaccgcgagg ggtccgccaa agcaggtcag
    11221 aattcccaat agtaggacga gccggggagc aaatggaagc ccaaagatca gcgtgagtcc
    11281 accgatggca agataaccta cccatcggac cgggccgaat gaccatcccg cagtgtagac
    11341 agccatagca aatgatagtc ccaggatcag ccatcctacg atttttagta ttaacatgga
    11401 cgggcttgaa ggaagatatg gcttgcccag catgacctga aaatgcttcg gcatggacag
    11461 gcacaaggtg aataacccgg cataagagag taggaatacg aggatatcca tgtcaggatc
    11521 ggctcgtttt gcgtttatcc acccgaagcg actgggtggg agggcctggg acgggatgat
    11581 gtacgtgcag gttggtcaag acgcctagcg gtccagccca atcctaatcc gcctatcaag
    11641 gccgtgaggt ccaccgcggc cacctcccac tgtccttttg gcactgtcat gaacagatgg
    11701 tgttccgtgg tgatgacatt cacgaccggc agcagtatga aaaatagggc cgcgacggac
    11761 aattgtgttt tccatattcg caaagatggc ctgagttgga aaagtttttt gattaaggca
    11821 tgccccaggc agagactcca ggccatgaaa aatatctgca actcccggag ttcacgtgca
    11881 ggaaggtcgg caggcaacag acgattggcc caaaagaacg tcgccgtggc caccaataat
    11941 cccgctatcg ccgccacgtt tactccttcc acgagccgat atcccactgc gtcggttgta
    12001 gtgtgacgcc cctgccgctt cacgacccac aggaccaacc ctgtggcaat catggcgctt
    12061 gccgccaatc ccatgccgaa gtacagccac cgtatagtgg caccgccgaa ttgcacaaag
    12121 tgcaatccgg agaggaccga ctgagtcata tacgcaggtt tggaattgat ccagacggtc
    12181 cggagtgcac cgcttgcccc atcgaaactc acgcggtcgc tatataagct caagcgatcg
    12241 ttcgactgac gattaacatg taccagggcg tgacgatcgc caggatgact taccacgatc
    12301 gacctggttt gacctccctg ccaatagggc cgggcctcgc gttccagatc tctcaatgaa
    12361 accagcggag ccggctgatt cgctctctcc ggttcaatga aggattcagc ctctgcccag
    12421 aagccatcca tctcgccatc ataatgaacc tgtaatcctg ccggcatgta gatcgaccaa
    12481 aagattacca ggccgctaaa ggtaatcatg atatgaaacg gaagggccag gacgcttgtg
    12541 atattgtgcg catccagcca ggaccgatga ggcgaggcgc ctgggcggaa ggtaaaaaag
    12601 tccttaaaaa tgcgatgatg aatcacgatg ccggtcacca aggcggccaa catggccatg
    12661 gccgcggcgc ccacgatcca agtgccggcc tggtccaata agagttgata gtgaaatcga
    12721 tagaaaaatt ctcctccatg agtgttacga acagtgatgg gctgtcctgt cttggcattg
    12781 atcatcctgg acacggacgt accgtcctgg ctccatcgaa tggtggagac cggtgtgcgt
    12841 tcgctaggca gctcaatcga ccagcgttcg gcctgcgggg caaactgttc cagggttcgc
    12901 atgacggcat ccacattaag cggggaaggg atgacatgat gcagttccgg ttgcatccaa
    12961 taggtgattt cttgatcgaa caccgtcagc gtgccggtga aaaagattgc aaacaacagc
    13021 cagccgaaga gcaggcctgc ccaggtatgg agccaatcca tgcactgtgt aaagctttct
    13081 ttcatggata agtgacttct ccgataggtt gtctcgactt gacagcccgc cttacgtcat
    13141 cagcaacgtc atgcccccca agagcagggt gggaatcaag aggacgaccc atgcccgcat
    13201 cggagtggcg gtcgcaaacg cgcagagaat ggctcccgta tagatcagga aggacatcat
    13261 gctggcagtc agcacagctt cggatttggc caaaggtagg accatggaca accacgccgt
    13321 gcaggcatac gccagcgcat atcccccaaa cactgcagcg gcaacccggg aagaagttgt
    13381 gatccaggtg ttagatgatt tcgttcgacg agctttggaa agcaaagata tggccttttg
    13441 ggctcctgac gtgaaagcaa aatggaaaaa cacctacgaa taggaggacg aacgaatttg
    13501 tcaaaacctt acgcattgct aagttttaat acgtagaatg cgccaagctc atccaaagaa
    13561 ccccaagcaa agcaagctaa ggggacgcca ggagcagttt tttatgaggt cggagatgcc
    13621 acctttcata gaagtccttt caatttccaa tctcttatct gtctccagac agtttgttat
    13681 cgatgacttc gatcatcccg gtctgtttcc gaggacttta ccgactcctt cgcattaagc
    13741 catcctgaaa tacttcttgt aatctagaca gccacatatt ttttttagga ctcgaaatgt
    13801 aaacaggctc ttcggtccat cttgctgacg gattgctaaa ttcccattgc aatttgtaat
    13861 gcagctgcct cagtgatccc ttcatctttg aaagggccaa aaaccgctac cgtccttttc
    13921 gaagaagtat cgggaaaatg atagacggca ccgacactgc ctgaaccggc agcggaatgc
    13981 ccgattgccc gtgtagattg tgccatacct tccctcccta tctgaccaat catgaatcca
    14041 agaccatagc ctgtcgtttt ccatgggcgc cccgggattt cacctccgac cggataacat
    14101 tgaagcatct cgttgagtga gtgggtccga agtaattttc cattcaataa tccatataag
    14161 ccaagtgcgg cttcggcaac agaacccata attagaccat ggtacaccca tccgggatgg
    14221 taaccatggt ctgtcttaaa ggtcgaaagt tccatgtcct cacgggtttc aacgactcgt
    14281 gcagagccta agtttagagg accaaaaacc aaccgatgga gacatcggga aagtggttcg
    14341 ccggtaatcc tttccatgag ccggcaaaca tagaagtacc caatattcga gtacataaat
    14401 ctttgaccgg gattgaaacg aaggttctcc acattcatgc gcgctagcag ctcctcggtg
    14461 gtccatggtt cttctcccct ggagacagcc tcatggtaag ccctgaggcc tccataattt
    14521 gcgataccgg ctcgatgttg taacagttgc cgaagtgtat agggttttcc ctccagcgag
    14581 ttgtctaaat tgacgtggcc ttgctcaaca aggcgaaggg cacaggcagc tattaaagtt
    14641 tttgtaaagc tccaccatgg aacgaggtcc tgcgatgact ctttcgaaag tacaccttga
    14701 tcgttaaccg atgccatgag catatttttt agatcggttt gcaatgtgaa tcctcaattg
    14761 atgggatttg tgtcaacatg gaataatttc aggcctttcc ctgccagata gcaagtttga
    14821 tacgggttac gttttgattg cttgaatttt tttcttcgct aaacgttcat tatcttgaac
    14881 gtaatgccat acgacttcat gcctgaagca atatggagga aagccgtcct ctgtatctgg
    14941 gatgttcagg ggatgggtgc aacaattggc gaaggggtgg cgattgtgac ctgcatgcct
    15001 tctatcggaa cgtctatacc ggacacgacg acctggtccc cggcctgcaa ccctccgcgg
    15061 acgacaagac gatcggtttc agatttcaat acatccaact gttgaagctt gagccggtta
    15121 gtgtcatcca cgatcaggac ttgctccgtc ctggtgttga gcacggcggg tggtaggaca
    15181 aagacctggt gatattcccg gccttcgatc tccgcctgta ggaatgttcc tggtaataac
    15241 ggtcgcccat ggtgttgatc ggacttcggt tgcgagacgc gtgcgacaag ggaaagctgc
    15301 ccggagcttg gttctaatgc gccttcggtt cggatgatgt atccaaccca acgacccatg
    15361 attccgccga tggtggtgtg cagggttacg gaaggtgccg tatgggttga tccgttcttt
    15421 tccgaaatcc atgggatgtc tataaatgct aattgttcca tggatacggg gagacgcact
    15481 tccaggctgt cagtggcata aaggcgggcg actttttctc ctggaacgat gtattggccc
    15541 atgtctacat gcttagcccg aattcgtcca tcgaacggtg cgcgtagcac gcagcggatc
    15601 tgctgaagtt tggcctttgc cccatccgct ttggctgcag ccacttttgc acgtatttcc
    15661 cgtagttgtg gtgtgcgcaa cgccaacgcg gttgcttcac ccttaccaag cacctgccat
    15721 tctttgagtg cttgttcgac ttgcgcctcc tcctgggcca aaagccgttg ggcttccgcg
    15781 agtcggcttt gggcttcaat ccgcgcgaat tgataatccc ggggatccac cgccacgagg
    15841 acgtctccgc gataaaaaga tccgccggcg gcgaaggcgg gatggacgtg aatcaccttg
    15901 ccagcgacct ctgtgaccag atcaatctct gtgcgtggca tcgccactcc ttgcgaggtc
    15961 acggtcacac gcacgctttg aggttggacc tcaatgacct caacacgtgg cggctccggc
    16021 tggagacttt ccgcctccgg ctgggagcga ttggtcagaa ggaggaaagc agtgagtaat
    16081 cccatcgata gcacgccgat gggcagcagt attcgcgtca tatggcgcct ccaagtgcca
    16141 gatatatatc aatgcggttg ttcagcaggc gccgtttgac ggtcaaatga gcactttgag
    16201 ccattaaggt tccccggaag ctgtcgagta acgtgagaat ggtcgtaaat ccatgacggt
    16261 aggcatagac cgccatctgt tgattggctt gggcatgctt caccgcctct gacaaagctt
    16321 gttcctgtgc cgataattgt tcttcggctg ccagtgcctg ttctacttca cggaatgcct
    16381 ccagagcggt ttgttgaaaa ttctccatgg cttcggtgac ggccgcgcgg ttacgtgcga
    16441 tctcaccgta gagttggtct ccggcaaaga tgggttggag caagccggct ccgacattcc
    16501 aggcaaggga cccgggctgc gtcagttctt tcagtgaatt actcctggtg ccgccggtcc
    16561 ccgttaaggt gatgcgcggc aatagcgcga ggttggcgct gatgaaccgc tggtcttctg
    16621 cacgcaggcg ttccatggct gcggcaatgt cgggtcggcg actgagcaat tcagacggaa
    16681 cccccgctgt aagcgttgaa ggtaatgccg gtaatggcaa ctgggcattc aattcgccgg
    16741 cgggataacg accaagcaac acttccaacc tgcgggcggt cagctggacc tggttctgtg
    16801 tgtcggccag ttgagcctca gcgttggcga ggtctgtcag cgccaatccc agatccaatc
    16861 ctggtgtgag cccgcgggta aaccgccctc ggaccaggtc cacaagcatg cgtcgatcgg
    16921 cgactgattg tgaggcgacc ttttcctgaa ggcctgcttc cagcaattca aaccaggatt
    16981 gtgctgttcg tgccgcgagc gataatgcgg ctccctgcaa gtccgtttcc acggccatgg
    17041 cgtccaatgc agccgcctcc tgcgaggcac gaattcgtcc ccaaatgtcc agttcccagg
    17101 aaaggttaaa aactacttcc atgaaactgt aattctcgtt ggcgacatcg ctatcgaatg
    17161 tcccgaaacg gccgtggcga aagtccgggg aaaaattgac ctgaggaaga cgaggagcgc
    17221 cagcgattcg cacattggct cgtgcaatct cgacccgagc cgctgcggcc ttgaggtcat
    17281 gattaaattc caacgcctcg cggaccatag ccgtcaatat cgggaggtga aacgtgtcca
    17341 cccaactatc cgtgacatgt tcgacggtcc tgtccgtaat ctttgaccat tgctcgggta
    17401 cctggctcat gatgacttcg ccatcacgtt ccggggtgga tagacatccc gacagaagga
    17461 tgagacagac tccggccgac atgatgagag gtttgcctgt ttcagacatg aggctatatc
    17521 ggcgaatcaa tgtccttggt gcatctaagc gctatagccc tcacaagagc gtggaatgcg
    17581 ttgttgtggg gttccgaatc agaatttgca aacacatact ccgggaggac tgacggcctt
    17641 ctgtttttca ccattgtgag cgatgagcca gtttgttcat gttcgatccg ttgagtcttt
    17701 ttacctaatg gcgagtaaga gtgaccacgt gggaattttg accccttccc atgtgtcttc
    17761 ctacctagga gacgtttgag tgggcccaat cccccacgag atggcatcgt ttgcgttcat
    17821 tcaagaatat tcgggtgcgt caccatgact tgagagggtc gcggggtggg ttggggacag
    17881 ggatcgttct aatatcagcg tcgtggccgg aataagtaat aacgtggcga ccaatcctcc
    17941 aaacagccct gcagctaatg cgacggccat gggaaccagg aactgagcct gagcactcgt
    18001 ctcaaacaac atgggcacga ggcccaccaa ggtcgtcaga aacgtcagca caatcgggcg
    18061 aaagcggatt tcgcaggcct ggcgaataag actttgctga ttaagggctg tctctttccg
    18121 cttgcgtcgt tgaacgtaat tgagcaagac caaaccatca ttaatgatga ccccactcgc
    18181 gccgatcatt ccgactaatg attccatgga caggggcaat cccaggacaa agtgagcgag
    18241 cacaccgcct acccaggcca tgggaacggc aaggaggatg agtaccgggt atcgaaatga
    18301 acggaatgag acggctaaga gtgcatagat gattcccacc gcaaatagag cattgcgtcc
    18361 gagtgtttca aaggctgcca tctggtcttg tcgcgtatgt ccgacggtga tattcaatcc
    18421 gggaaaacgc tgtttgaggt tttcgaaaaa ccctgtttgc atcgttgcat agatggcatt
    18481 ggcatcggca agttccggat tgagccgagc tctaacccag accaactgtt gtcggttctg
    18541 tcgtgtaatg ctctccgttt gagggagcca acgccatgtc gccaggactt ccaggggggc
    18601 ctggtcaccc gtgggcaaca ggattggtaa ttgccgaaaa tcattgaaat cacgtcgctc
    18661 gttcaggggt aggcgtacca cgactttgac ctgttcacgt tgtcgctgga accgttgcac
    18721 ctcctccccg agataggcct gacgcacttg tgtggccacg tcctcaagtc gtagcccgag
    18781 tcgctcggcc tccggtcgaa gttccaggct cagctgtggt ttgcctttga tttgtgaatg
    18841 catgacatcg aacaccccag gaaggctgga aaggtgttcg gtgagtgtag ccaacgcagc
    18901 ggtcatggtt tcagagtcgg tggcgctgac ggcccattcc aggtcgtagt aattgtctcc
    18961 ctgtcggtac agaaagtcga gtttggcctt gccgatatcg ccaatatggg ttcgccattc
    19021 ccgcgcgatg gtttcgactg aaaatcgctg tcgagcacct ggggccaact cgacccagaa
    19081 tcctgacccg ttatcgaaga tcatgctctc aatccccgag acggggctgg gctcttcagg
    19141 tggttggcga gtttccagtg aatgacgtaa ttccttgtag gcctcttcaa tctgggtgga
    19201 taatcgttgt acggtcgtaa atggcgcacc aggggggacg tcgagataca gggtaatcgt
    19261 atctttcggc acatcggctt ggatggctag ccggacatgg ccacctgaaa ccagtgcggc
    19321 ggtgatcatg attgctgaag cgcagaacgc gatgatcaga tagggttggc gacaggaccg
    19381 atccaacaac ggcaaatagg cacaatggat gaaagcctcc agcccgcgag tcactcgttg
    19441 tcgaagggtg gccatccatc ggcttgatac gggaacgggt gtggtcagat gagcaggcag
    19501 aatgcacagg gcctcgacaa g
//
LOCUS       DAORAQ010000043        18278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_539_length_18278_cov_67.534160, whole genome shotgun sequence.
ACCESSION   DAORAQ010000043 DAORAQ010000000
VERSION     DAORAQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 18278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18278
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_539_length_18278_cov_67.534160"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKM72_06035"
     CDS             <1..778
                     /locus_tag="PKM72_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNP60379.1"
                     /translation="YLAPVGNGLTIQAGLFNSFIGYESLYAKDNFNYTRSWVADYSPY
                     LMFGANAVYPFNDRWTGAVFIINEYFHLQDANNLPSYGAQTIYKPGEPWTIKETVYYG
                     PDQSNTSLEFWRFFSDTIVEWKSKEVTIAGQYQIGTQKNATVPGNPRLMYMGAALHMR
                     WHLKKSWFLALRPELYSDPNGLITGSEQFVWAVTATAEYRLPYEWTNSIFRLEYRHDN
                     STGPGGGFFKGAGNRLTPSQNLLIFSVIWTFDSQCSNTPC"
     gene            complement(878..1405)
                     /locus_tag="PKM72_06040"
     CDS             complement(878..1405)
                     /locus_tag="PKM72_06040"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HNP60380.1"
                     /translation="MSTTKLSSGSRLPAFTLPLVSGGEVTLGQPEKKGNWQVVFIYRG
                     LHCPICKKYLTKLESLKDKFPATKTEIVAVSGDSESKAREMAETAGVTFPVAYGLSIE
                     QMKELGLYISHPRSPKETDQPFPEPGMFAVNPEGKVQLIDISNTPFNRSDPDELVDTL
                     RWIQENDYPIRGTYE"
     gene            1615..2487
                     /locus_tag="PKM72_06045"
     CDS             1615..2487
                     /locus_tag="PKM72_06045"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HNP60381.1"
                     /translation="MKSIPNWPVDETCIKEGILTLLDPSLDSRFSPKRLTASFWVKVA
                     ALMTLSVTIYVVMQTINIGEVFNPDRITQWLSQAGPLAPFLFMGLMALAVIISPIPSL
                     PLDIAAGAVFGPLLGAMYAVIGAEVGAITSFYIGRAVGREVLTRLLHINIAFCEKCSD
                     RHLAIFVFLSRLIPIFSFDLISYGAGLTNMSIRVFALVTFLGMIPPTLALTYAGSYVG
                     SGTWLTVVLGLAMVALLLLIPSIVLRYPTSRFVKLLRGETSRSTPAPVPLQQPIATSD
                     QSQPRCDSCHGKME"
     assembly_gap    2496..2595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2695..3579)
                     /locus_tag="PKM72_06050"
     CDS             complement(2695..3579)
                     /locus_tag="PKM72_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP60382.1"
                     /translation="MANLSKRLQTNIEGNFFVDSTCINCDTCRQLAPLTFIEDGDYST
                     VFRQPETAKEEFAAYQALIACPVGSIGTEKKDRKVFLEAQASFPLHIEGGVDYVGFNS
                     EKSFGANSYFIQHSQGNWLVDSPRFLKHLVQAFEEKGGIRYIFLSHEDDVAEAARYAK
                     HFDAIRIIHRADASAMPGAEHVIDGENSVHVAPEFVCIPVPGHTAGSMALLYKDRFLF
                     SGDHLWWNPNLQQLGTPERLVWDDIQLKRSVKKLLTHSFEWVLPGHGERIHLPHRDMK
                     KALDQLLQRRWNFQSLPL"
     gene            3770..4204
                     /locus_tag="PKM72_06055"
     CDS             3770..4204
                     /locus_tag="PKM72_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60383.1"
                     /translation="MQKIAAMVLTAALFTGFSGCSYVFYPRADDYAAKAKGSTSVETV
                     LNLTTMMEASAEAAKGGTGNDQSMDDLHNQFHAFDNSLCCVDEAKRETPTYALAVTHN
                     KELWAIFKRLWKFKDTQPQRDEHLELFKREIQELRATLEALK"
     gene            complement(4306..4995)
                     /locus_tag="PKM72_06060"
     CDS             complement(4306..4995)
                     /locus_tag="PKM72_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HNP60384.1"
                     /translation="MKYLIYLVFLSFSCFGLTTQPLAAVGEVNYISDVVTVPLRSGPT
                     TAHRILHRGLPSGTQLTILAVDEEAGFTQVRTTDGMEGWITSQYLVGEPIARVKLAAT
                     EKRLQSLKAEIDKEREARASIQAEHKETDANNRTLNSQVQSLSKELTELKRISADSIN
                     EHARNIELTQQNTRLAGQVEELSSKARQLEENLQLKWLLYGGGLVLIGLLIGVILKSR
                     PRQTTSYTRYT"
     gene            complement(5556..6170)
                     /locus_tag="PKM72_06065"
     CDS             complement(5556..6170)
                     /locus_tag="PKM72_06065"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNP60385.1"
                     /translation="MKQFVPVGIVLILGLGSLAGWAMTNEAKSFAPNVHPKTGAISVP
                     ENYREWPTLGTWAHAKVEGAPGLQEYHVVYTQPDTIKYYNENNRFPDGAVLVKELLNA
                     DTMPMTTGPAVGHATTIKGWFVLVRDTNGRFKESSLWGDGWGWSLFDPDDPQHTVSKD
                     YKIECLPCHTPARELAPHRAVDADKWIYSFGYPVLQKEMREKLK"
     gene            complement(6251..6712)
                     /locus_tag="PKM72_06070"
     CDS             complement(6251..6712)
                     /locus_tag="PKM72_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HNP60386.1"
                     /translation="MKMMQTVLGSLFAMFLVIVATPAFAGDVTNNTPGISGYDPVAYF
                     TEGKPMRGSGYHVTEYKGVTYAFATKDHKEMFEASPGKYVPAYGGYCAYGVAVGKKFV
                     SDPEAWKIVQGKLYLNLDRDIQSKWAKDIPGYLKKSEANWKDIKDKAPSDL"
     gene            6955..7551
                     /locus_tag="PKM72_06075"
     CDS             6955..7551
                     /locus_tag="PKM72_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNP60387.1"
                     /translation="MKRKSSSPSKREQLIQTAVTLFAQNGIHATGIDTIVEQSGVTKK
                     TLYAHFRSKEELVLAALRHYDGQFRNSFMRQVETKARTPKTRLLAIFDVAEIWFSQQN
                     FYGCMFINAVGEHSTPDTSLRYVCSDFKRMMTEFILGLCIKMSARNPQQLAEELALLL
                     EGAIVTAQVSQKSDAAKIAKRAAAVLIENQSSYETLQT"
     gene            8757..9743
                     /locus_tag="PKM72_06080"
     CDS             8757..9743
                     /locus_tag="PKM72_06080"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNP60388.1"
                     /translation="MPEIQRIMSGTWKLLKSLFDSQEATALASHKNGRDIDLARTVPF
                     LALHIAVLAVFWVGWSPIAVGVAVSLYIIRMFAITGIYHRYFSHRTFRTSRGMQFVFA
                     CLGASAAQRGPLWWAANHRHHHAYSDKPEDVHSPVLRGLWWSHVGWFLTHDQLATKMN
                     RIKDFARFPELRFLNRFDTLVPIGLAVILFLLGKWLGSAYPNLGTSGWQMLVWGFVIS
                     TVVLWHGTFTINSLSHVFGTRRFATSDHSRNNFLLAIITLGEGWHNNHHKYCSSTRQG
                     FVWWEIDVTYYVLRCLQFFGVIWDIRPVPAWVLNQTDLEEIGQDAESEKMAM"
     gene            9955..10977
                     /locus_tag="PKM72_06085"
     CDS             9955..10977
                     /locus_tag="PKM72_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60389.1"
                     /translation="MFRACIPLMTIVFVTALTFLLGNQTAWAFSIIEPKEFTVHQSGE
                     HIPVTLDLEGLPGVTTVKYYWYGEFDDMLRELVEEKLALVATAKSSPPFGGKILIPKE
                     RMGSYRLLAVAEQGGRQSQHESLAIFDEIMIQIEPNAELLEIDFQTDKPLRLGRASGT
                     RVYDQVDFLGKTFELPVIGRFSDGIVRSIRAQTTGTTYQSANDKVISVNPNGVLRLMG
                     NGETVLTVKNRNQEATLDIMVEVNDEPNHPPVSDPGQTQTVLSGTRVTLNGLTSYDPD
                     GGSLQYHWEQVRGSKVPLLDPYSAQAKFLAPFVVEERLFRFMLKVTDIQGADSLPAFV
                     DVIITP"
     gene            11776..12729
                     /locus_tag="PKM72_06090"
     CDS             11776..12729
                     /locus_tag="PKM72_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60390.1"
                     /translation="MLKSSWWEGSWFVWPLMAFTILTACLGKFETKPTLTDPCRLDWQ
                     KQAGIAKADLPDSTVNRGLPVLVFNIHDRLLSDSFFSSEAHTEGEVACIGDLASMFDL
                     VLFQEAFVRPAQLAHYTKHAWANHPLFTEGGGGDWWPLRAMCNICLSPGLLMLAREQP
                     ESIYSEPYEDFAGWNTDLNKADDFFSKGFQLVKFKKFWVLNSHMDAGRGEASIEARAL
                     QFRQISAGIRRLVSPDAPLLVGMDANLRPDKGERDAEILEEFLNANGLTLVQQSGPDL
                     IAVRGIRTDRPKSLPLKGILSDHNALSVIMYPPSRQESQNQ"
     gene            complement(13092..14684)
                     /locus_tag="PKM72_06095"
     CDS             complement(13092..14684)
                     /locus_tag="PKM72_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60391.1"
                     /translation="MDEMNRQGIPRAMIGCFVLQIGLLIWAGWVNQDWISTDGISYIR
                     IAQYYASGQFDLAVSGYWGPMLSWIIIPLLGLMEEPLSAAQMAMGISAIVYLMGCMAI
                     FRVLGIGSSAILVALGIVALMSVHWAVQGITPDLLMSGILCMAISRTLQPDWCDKASV
                     QFSAGLLFGAAYLAKAPGLPFAFILIMAAGLLWTVSQETSLTRALRSVAITIIGLMLI
                     AGPWILILSLKYGKPVFSTSAGISHAVMGPPGVGRGHPTFSDFVIPEQGRVSSWEDPI
                     SKKYSHWSPLESTENLRHQLKIVHKNVMKTIHLLKDFDWLGIGLVSVVFGFLFHAPWR
                     QNIRSDRWRWAAIFIGGLVVIFLPGYAGEKRYYYSVYPFLLGGSLGYVGHLAKSRTSQ
                     AKILHFMALAIISLSFLFPLANSSFFPQRPSYQAAKVLQEKLSTLNISGPIASTSRFE
                     NIDAYTAFLMQVPWHGRSERSQTIEEVESSLAKIIIVDRGTLADENLRNDASFIGLDE
                     KMFDSLGNDFDTRVNVYINTKI"
     gene            complement(15250..16236)
                     /locus_tag="PKM72_06100"
     CDS             complement(15250..16236)
                     /locus_tag="PKM72_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HNP60392.1"
                     /translation="MLSTQPSSFFRILWPYVQIARIDHWFKNVFMILGIVLALFYELE
                     LFAWESVFPLLLAIAATCLIASSNYVVNEVLDAPYDRLHPVKKDRPVPSGKVKTSWAW
                     IEWFLLGTSGIVLAFSLNEYFGTTALVFLVSGLIYNIPPIRTKDLPFLDVLTESLNNP
                     IRLLLGWFALVTHSIPPLSLVLAYWMVGAFFMATKRFAEYRRIDDPIRAKAYRKSFGY
                     YTENRLLLSMFFYAMACSFVSGIFLVRYHMELILFVPIGAGFLTYYLKIGMLKDSPVQ
                     NPEKLYKEGGFVVYVGLSTLLFLLLMFSEIPFLYELFNVNPAIIPPLWTVGE"
     gene            complement(16214..16942)
                     /locus_tag="PKM72_06105"
     CDS             complement(16214..16942)
                     /locus_tag="PKM72_06105"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNP60393.1"
                     /translation="MKQVQQIRAVLLDIDGTLYHQKALRGLMAFELATAPFSLGSIPE
                     ALGVWRILKCFREGREALRAMKDPSIPLASLQYSLVAERFCVTSREVELIVREWMYLR
                     PLKYLKICRRRGMVEFFQLADEMGLRTGVFSDYPVREKLQELGISVPLTVELCGTDPE
                     INAFKPNPKGFLYACHLWNLDPGEVLYVGDRHDVDACGAKAAGMPYVIFSRRASRGFN
                     ASATTELRSITNFRGLCDVIHSAI"
BASE COUNT         4867 a         4556 c         4166 g         4589 t
ORIGIN      
        1 ctacctggct ccggtcggca acggcttgac gattcaggcc ggactcttta acagcttcat
       61 cggttatgaa tccctctacg ccaaggataa tttcaactat acgcggtcct gggtggccga
      121 ctactcaccg tacctcatgt tcggagcgaa tgcggtctat ccgtttaacg accggtggac
      181 cggtgccgtc tttatcatca atgaatattt ccatcttcaa gacgccaata atctgccgag
      241 ttatggggca cagaccatct acaaaccggg tgagccctgg acgataaagg aaaccgtcta
      301 ttacggacct gatcagtcga acacctcact ggaattctgg cgattttttt ccgacaccat
      361 tgttgaatgg aagagcaaag aggtcacgat tgccggccag tatcagatag gaactcagaa
      421 gaatgccacg gtgcccggca accccagatt aatgtatatg ggagcggccc tccatatgcg
      481 ctggcacctt aaaaaatcct ggtttctcgc actccgaccg gagttgtatt ccgacccgaa
      541 tggtctcatt accggttctg agcaattcgt ctgggcggtt accgccactg cggaatacag
      601 gcttccatac gagtggacca attccatctt ccggctggaa taccgccatg acaactcaac
      661 gggtcccggt ggcgggttct tcaaaggcgc gggaaatcgt ttaaccccct cgcagaactt
      721 actgattttt tccgtcattt ggacattcga ctcacaatgc tcgaacacac cgtgctgaga
      781 tcactgagag aagaggcgca ttaacttggg aagaagccgg ctcacaaaat tcgagaaagc
      841 tgttgtgaaa aaatttctgc atccacccag cggaaactta ttcatacgtt ccgcggatcg
      901 gatagtcatt ctcctgaatc cacctcaacg tatcgactaa ttcatcggga tcggatcggt
      961 tgaacggagt gttggaaata tcgatcaact ggactttgcc ttccggattc accgcaaaca
     1021 ttccaggttc ggggaacggt tggtccgttt ccttcggaga gcgtggatga gagatataca
     1081 gcccgagttc cttcatctgt tcaatggaga gcccgtaggc caccgggaac gtcacacctg
     1141 ccgtttccgc catctccctg gctttgcttt cggaatctcc tgagactgcc acgatttcag
     1201 tcttcgtggc cggaaactta tctttcaagg attcaagttt cgtcaggtat tttttgcata
     1261 tcggacaatg caggcctcga tagataaaca cgacttgcca gttccccttc ttttccggct
     1321 gacccaatgt gacctccccg ccactcacca gcgggagggt gaatgcaggc agtcggcttc
     1381 ctgacgaaag ttttgttgtg ctcatccatc tctccttctc tgaggattca ttttccccag
     1441 gacttttctt ttaaatcgat aactggatcg cctcatttgg cgacccttaa aagatcccgt
     1501 tgattttaca ttaacaagcc cgacctatcg ggtaatctct tctgtttgga taaaagttca
     1561 ttttttgatg gaactttgtc taacaacatg ggaatattcg tgattcaatc ctgaatgaaa
     1621 agcatcccga attggcctgt tgatgaaacc tgtattaaag aaggcatcct gacattgctc
     1681 gacccatccc tagattcccg tttttcgcct aagcgtctga ccgcatcatt ttgggtcaaa
     1741 gtggcagcgt tgatgacctt gtcggtaacg atttacgtgg tcatgcagac catcaacatc
     1801 ggcgaagtat tcaatcccga ccggatcacc caatggctgt cacaggctgg ccctttggcc
     1861 cctttcctgt tcatgggatt gatggctctc gccgtgatca tcagcccgat tccaagcttg
     1921 cccttagaca ttgccgcggg tgcggtgttc gggccattac ttggtgccat gtatgccgtc
     1981 atcggagcgg aagtcggtgc aatcacgagt ttttacattg ggcgagccgt gggtcgggaa
     2041 gtgctgaccc gactactcca tatcaatatc gccttctgtg agaaatgctc ggaccgtcat
     2101 ctggcaattt ttgtgtttct atcccgtctg atcccgatct tttcgttcga tctgatcagc
     2161 tacggtgccg ggctgactaa catgtcgatt cgtgtctttg ctctcgtgac gttcctggga
     2221 atgatccctc cgacccttgc tctcacttac gccgggagct atgtgggctc aggaacttgg
     2281 ctcaccgtcg ttttgggact tgcgatggta gcattactcc ttctcattcc ctcaatcgtc
     2341 cttcgatacc ccacttcccg tttcgtgaaa cttttgcgag gggagacctc aagatcaacc
     2401 ccagcccctg tcccactcca acaacccatt gcgacctccg accaatctca accacgctgc
     2461 gactcatgtc acggaaagat ggaatgagac cctcgnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnaccaa tctcaaccac gctgcgactc atgtcacgga aagatggaat
     2641 gagaccctcg gtttttgtgg gataagtttc ctctagttca cgaagaaggt cgagtcaaag
     2701 cggcagggac tgaaaattcc accgtcgttg cagtagttgg tcaagggctt ttttcatgtc
     2761 tcgatgagga aggtgaatac gttcgccatg gccgggcagg acccattcaa aggaatgggt
     2821 gagcaatttc ttgaccgatc gtttcaattg tatgtcatcc cataccagcc gttccggcgt
     2881 cccgagttgt tggagatttg gattccacca cagatgatcg cctgagaaca aaaaacgatc
     2941 cttatatagc agagccatgc ttccggcggt atggccggga acaggaatgc agacaaactc
     3001 cggcgcaacg tgtacggaat tctcaccatc aataacgtgc tctgcgccgg gcatggcaga
     3061 agcgtccgca cgatgaatga tccggatggc atcaaaatgc tttgcataac gagccgcttc
     3121 cgccacatca tcctcgtgac tgagaaagat gtaccgaatc ccgcctttct cttcaaatgc
     3181 ctgaaccaga tgcttgagga aacggggaga atcgactaac cagtttcctt gggaatgttg
     3241 gatgaaatag ctattggctc caaacgattt ttccgagttg aatcccacgt agtccactcc
     3301 gccttctata tgtagtggaa atgaagcttg agcctccaag aataccttgc gatccttctt
     3361 ctccgtgccg attgatccca ccggacaggc gatcagggct tggtacgcag caaattcctc
     3421 ctttgccgtt tctggttgac ggaagacggt tgaataatca ccgtcttcaa taaacgtgag
     3481 tggagctaat tgccgacagg tatcacaatt gatacaggtg gagtccacaa aaaagtttcc
     3541 ctcgatattt gtctgtaatc gcttggagag gtttgccata attcgatgct cttactggtt
     3601 cagtataccg ggcagaaaaa cgtccatgcc atattaagaa aaagaggttg tatggccctg
     3661 tgttccaaga tacgctaaca ttcagattat caagaatgac caaaaccgac acttacagga
     3721 catagaaaac caaacgttat tgtaccaatc ctaaacgacg gagggattta tgcaaaagat
     3781 cgcagccatg gtcctgacag cggccttatt caccggattc agcggatgca gttatgtatt
     3841 ctatccgcgt gcggatgact atgcggcgaa agcaaagggg agcaccagcg tcgaaaccgt
     3901 gctgaatctc accaccatga tggaagcgag tgccgaagcg gcaaaaggcg gaacagggaa
     3961 cgatcaatca atggacgacc tgcacaatca atttcacgcc ttcgacaatt cattgtgttg
     4021 tgtggacgag gccaaacggg agacaccaac ctacgctctc gcggtgaccc ataacaagga
     4081 gttatgggcc atctttaaac gactatggaa attcaaagat acgcaacccc aacgagatga
     4141 acacctcgag ttatttaaga gggagatcca ggaactccgc gcgacactcg aggcattgaa
     4201 ataaacgggc gacgccaatc cacccgactg gccttgatcg aatgtgtgcg agatgggtgc
     4261 ccaaggaagg ggaaatttgc gtcacggtat ttttaaggtt atcacctagg tatagcgggt
     4321 ataactcgtc gtctgtcggg gccgtgactt gagtattact ccgattaaca gaccgatgag
     4381 taccaaaccg cctccataga ggagccattt gagttgtaag ttttcttcca gctgcctggc
     4441 ttttgacgat aactcttcaa cttggcctgc taagcgggta ttctgctgcg tcagctcgat
     4501 gttccgggca tgctcattga ttgaatcggc cgatatacgt ttcaattctg tcagttcttt
     4561 ggacagggat tgcacctgcg aattcagtgt ccggttgttt gcgtcggttt ctttgtgctc
     4621 agcctgaatg ctcgcacgcg cttcgcgttc tttatcgatt tcagctttta ggctttgaag
     4681 gcgtttctcg gttgcggcca gcttgacacg agcgatgggt tctccgacga ggtactgaga
     4741 cgttatccag ccctccatcc catcggtggt acgaacctgg gtgaaaccgg cttcctcgtc
     4801 taccgcgaga atggtcagtt gcgtcccgct ggggagacca cggtgtaaga tgcgatgagc
     4861 agtagtgggc ccactgcgta gcggcacagt taccacgtca gaaatataat tcacctctcc
     4921 gactgcggcc aatggctgtg tggtcaaccc aaaacaggag aacgataaaa aaacgagata
     4981 gattaaatac ttcaaagcgt ttcgtctcct tgttgtgtcg cacatctcga aaaaggagtt
     5041 cttcgggaga aaggtagaac gccatactca cagggaatgg gaaatataga tgccctggga
     5101 gacaagccga taagtagcaa aggagccgcg aggtgtcaat gacgccaaaa attcggctcg
     5161 tgaaagtgga ggctcaaaaa ggttcatttc tttattaact atccattaat cccccttttt
     5221 tatccacgaa tataggagat ctgcaaatgg tcaagccaat aggtctagag agggtgggtg
     5281 ggtgaggtag gatgaaatga aagacttcac cacaaaactt ttttctgact ccggtgatca
     5341 tatgacccac cacctgaaaa tgggttggtc gagcttacgc tcaagtcaag gagcggacca
     5401 tttttgagca cggcgaattg gtccgcccct ccacagtacg cgtcgggaag atgcaatgtg
     5461 ccgacttggg tgacaatatt ttaaggtcat tttccgccca aaagtgtcgg gcgaataaac
     5521 ctctttatgg aaaattgccg ttgaattgcc tattcctatt taagtttctc tctcatttcc
     5581 ttttgaagca cagggtaccc aaaggaataa atccatttat ccgcatccac agccctatgc
     5641 ggcgccaact ctctggccgg ggtatgacaa ggaagacatt ctattttgta atccttggac
     5701 accgtgtgtt gaggatcatc cgggtcaaag agagaccatc cccagccatc accccacaga
     5761 ctggattctt taaaccgccc gttcgtatcc ctgaccaaca caaaccagcc tttgatggtg
     5821 gtcgcatgac caactgccgg cccggtggtc atcggcatgg tgtcggcgtt tagaagttcc
     5881 ttcaccaaca cggctccgtc ggggaaccgg ttgttctcgt tgtaatactt gattgtgtcc
     5941 ggctgggtat acacgacgtg gtattcttgc aaacctgggg caccttccac cttcgcgtga
     6001 gcccacgtac caagtgtggg ccattcccgg taattttccg gaacactgat ggccccggtt
     6061 ttggggtgaa cgttgggggc aaaagacttc gcttcattcg tcatcgccca ccctgccaga
     6121 ctccccaacc ccaggatgag gacgattccc acgggtacaa actgtttcat gtacttctcc
     6181 ttcacaattg gcatctataa aacggctgag gtccgtcaca tagaccatga cggacctcgg
     6241 ctttgttcct ttataaatcc gacggcgcct tatctttgat gtctttccaa ttggcctcgg
     6301 acttttttaa atagcccgga atatcctttg cccatttgct ttggatatcc cgatccagat
     6361 tcaaatacag tttgccctga acgatcttcc aagcttccgg gtcggacacg aattttttcc
     6421 cgaccgccac accataggca caatatccgc cgtaggctgg cacatacttc ccggggctag
     6481 cctcgaacat ttctttatga tctttagtgg caaaggcata ggttaccccc ttgtactcgg
     6541 tcacgtggta accggagccc ctcatcggct tgccctcggt gaaataggcg accggatcat
     6601 acccgctgat tcctggagtg ttattggtga catccccggc gaaggcgggt gtggccacga
     6661 tgaccaggaa catggcgaac agagaaccta atacggtctg catcattttc atgatggatc
     6721 tccctttcaa aggtagaatg gttggtaaaa ttaagtatac agatctgtat cctttatgga
     6781 gcctagtcag acgagttctt gtttcgttac acttcttttt tcagccgatt tgacctggca
     6841 tactcaattt aaatcattgg tatatataga taaaaatgat agatgtttgt caggaatact
     6901 tgttatccac ataatattta gaaaatatat tggtagacgt aaaggtctag tagagtgaag
     6961 cgaaaaagca gctcgccctc caaacgggag caactcatcc agacagctgt gaccttattt
     7021 gcgcaaaacg ggattcatgc cacgggaatc gacaccatag tggaacagtc gggggtgact
     7081 aaaaaaacct tgtatgcgca tttccgctca aaagaggaat tggtactcgc ggcattgcga
     7141 cactatgacg ggcagtttag gaattcattc atgcggcaag tggaaacaaa ggcgcgcact
     7201 cccaaaactc gcttgctggc tattttcgat gtggcggaaa tctggttttc gcaacagaat
     7261 ttttacggat gtatgttcat caatgccgtt ggtgagcatt ccacccccga tacctcactc
     7321 cgttacgtct gcagcgattt caagcggatg atgacggagt ttatcctcgg gctatgcatc
     7381 aagatgagtg cgcggaatcc ccagcagtta gccgaagaac tcgcgctcct attggaaggt
     7441 gcaatcgtga cagcccaagt gtctcaaaaa tctgacgcag ccaaaattgc caaacgcgca
     7501 gcagccgtgc tgatcgaaaa ccagagttcg tatgaaacac tccagacata agatcacatt
     7561 tcgatgtgaa ttatcagata tccctcattg ttttggggat catcactcgt caaatacagc
     7621 ttccttaata ttaggccatt agcttggtcg ttgggcctcc tgtgaacaac tcaataaatt
     7681 cagacatgac cgtgctgatt tctgttgatg tgggagattc ccttatgttt aggaaaagaa
     7741 tgttcgatca acaagcccat tccacaatca gaagccagcg gggttgaaat cgtgaagggc
     7801 aggaaggact tcatgtcata agccccatct tgaatattgt cctgtggggc accgtatttg
     7861 gcgggaaggc ttcaggtgag gccaacaatc ctatgggctt cctggattta cttacaacga
     7921 aagcacaggg attgtcctct tatgatccag cgaaatacca gacagatacg atgaaagacc
     7981 cattttgagt gcattttttt gcctcatcca caatccagga cctactggca cgggagaagt
     8041 tctcagtcac actcaagcgg tgattataaa tacttcagag tttccatagt tgccccatgc
     8101 tcatgtcctg gaaaacaaat tagtaaatct gtaggtgttt ttgttatact atgcccatac
     8161 atcggactca gtaggggggt tcacaagagc gggtgcatct ttcgtgagct tgccctgcac
     8221 cttccaaaat aaatattcat acattcctcg aaactgaata gtcattcctt gaatttattc
     8281 cacagtttcg agcagcttac caagatcgga cccattgaac ggagcgtttg acatatcaac
     8341 caggcccagt tatccgtccg catccatgac aacagaccaa acccggaaca ccataacttt
     8401 ccacgcccaa taagtagaaa attcatggga ggaaatgagt cttgaaaaaa gttttggcgt
     8461 gcctatgatc aatcttcaaa ttcttcagtg tcaccacgat gggtactttg ggcaggcttt
     8521 ttcaaccgat tcttatcgac tatccagcct cccatcaaat actaacggcc atacacactg
     8581 tgaaattgta tttgccagaa gctctccttc tggtttactt tgtagagttt gaacatttcc
     8641 ctttatctta agaaagttgt ggatccctgt tttacacaat ggcttcaatg aaattcaatg
     8701 agtcgtaatc tgtaaatatc tcgggattcc ctacacatga gagaggagcg ttaaggatgc
     8761 cagaaatcca gcggatcatg tcgggcacat ggaagctgtt aaagtccttg tttgacagcc
     8821 aagaagcaac agcactggct tcccataaaa acggtcgcga catcgatctg gcacgcacgg
     8881 tcccgtttct ggctttgcat attgcggtcc tcgcagtctt ttgggtgggt tggagtccta
     8941 tagcggtcgg ggttgccgtg tcgttgtata tcataagaat gttcgctatt acgggtattt
     9001 atcaccgata tttctcccac cgtacgtttc gcacctcacg agggatgcag ttcgtatttg
     9061 cctgccttgg ggccagcgcg gcccaacgag gtcccttatg gtgggccgcc aatcatcgcc
     9121 accatcatgc ctattccgac aagcctgaag atgtgcactc tcccgtcctc cgcggactgt
     9181 ggtggagcca tgtcggctgg tttttaactc atgaccaact tgcgacgaaa atgaaccgta
     9241 tcaaagattt tgcgcgattc ccggaattac ggtttctaaa tcggttcgat acacttgtgc
     9301 cgattgggtt ggcagtgatc ctgtttctat tggggaaatg gctcggtagt gcctatccaa
     9361 atctaggaac aagtgggtgg caaatgctgg tgtggggctt cgtgatttcg actgttgtcc
     9421 tgtggcatgg cactttcacc atcaactcgc tctctcatgt tttcggcacc cggcgttttg
     9481 ctacatccga ccatagccgg aacaacttcc tcctagccat catcacatta ggtgaagggt
     9541 ggcacaacaa tcatcataaa tattgcagtt ccacccgtca gggatttgta tggtgggaaa
     9601 tcgatgtgac atattacgtg ctgcgatgcc tgcaattctt cggagtcatc tgggatattc
     9661 gtccagtgcc ggcatgggtc ctgaatcaaa cagaccttga agaaattggc caggacgctg
     9721 agtccgaaaa gatggcgatg taagacccgg caataccccc ctggatggtc tttgaaggcg
     9781 ttcgaccaca tttatacgcc atccctttta tatatttgga tctccaggcc gtccctttct
     9841 cactctccca catctcacaa gctaaaaact cgtctttatc atgggagatg cacccacttg
     9901 gaagggtgat gtatgattag actgttagct ccttatttct tgtgaggtac gatcatgttt
     9961 cgcgcttgta tccccctcat gacgatagtt tttgtcaccg ctcttacctt tttacttgga
    10021 aatcaaacag cgtgggcctt ctccataatt gagccgaaag aattcacggt tcaccagtct
    10081 ggagaacata tcccggttac cctggattta gagggtctcc caggagtcac cacggtgaaa
    10141 tactattggt atggggaatt tgacgatatg ctgcgggaat tggtcgagga aaaattagcc
    10201 cttgtcgcca cggcaaaaag tagccctccc tttggcggaa agatcctaat acctaaagaa
    10261 aggatgggct cctatcgact tctggctgta gccgaacagg gtggaagaca atcccagcat
    10321 gaatccttgg ccatcttcga tgaaattatg attcagatcg agccaaacgc cgaactcttg
    10381 gaaatagact ttcaaacgga caaacccctg cgtctaggtc gagccagcgg gactcgtgtc
    10441 tatgaccaag tggactttct cggaaaaacc tttgaattac cagtgattgg gcgattctca
    10501 gacggaatcg tccgctccat tcgggctcaa accacaggaa ccacctacca gtccgccaat
    10561 gacaaagtta tttccgtcaa cccaaatgga gtactccgac tgatgggtaa tggggaaacc
    10621 gtactcaccg tcaaaaaccg caatcaagag gccaccctcg acatcatggt tgaggtcaac
    10681 gatgaaccca atcatccacc ggtttcggat cccggccaaa cgcaaacggt cctttcggga
    10741 acacgagtca ccctgaatgg cttaacgagt tatgatcccg acggcggttc tcttcagtac
    10801 cactgggagc aagtacgcgg aagtaaagtc ccattgctgg acccgtattc ggctcaagcc
    10861 aaatttttag ccccatttgt agtagaagaa cggctcttcc gtttcatgct caaagttacg
    10921 gacattcagg gcgccgatag tttacctgcc tttgtcgacg tcatcattac gccatagcga
    10981 aaggcaccta ataggacgta cagttcgaga tagaaaccac aggaagcccg atacggtcag
    11041 cttctttccc tccttgactc tctgatctct agatcaaccg taagcctgac agggaaagca
    11101 tccctattct gttcgctata ttttgccatt caccagaaag cgtttgagaa catcatcccc
    11161 aatatggaac acgaggatcg tgacctagaa tccaagaaag aaaagacgga aatgaaaacg
    11221 ataccctttc taagctagcg gaaatgtcag gtaaaatatc tttgcggcca gaaatggttt
    11281 tatttgactc ctatccaagt agggcctgat ccaattcctc aaatcaaaaa tctaaaagta
    11341 ccctgcccac tctatgccat gaaatcaaaa tacgacagat gccatgtgaa ttgaggttac
    11401 agagaaatgg tcagatgacc gggagtcaga tcgttaattt ggagagtgtg atgctgcggg
    11461 aaagggaggg ggaataagac gatttaacat gcacctgaat ttacgattca tgagcgtcca
    11521 catgtagaaa ttgaacctcc agcctgatcc tgcaattgtt tttcgtgttc atctcaggga
    11581 aaaatgcctt ttttaagaat gcagaaattt catccccatt gaaatccaaa agtatgggtc
    11641 cccgacctca cacggctact agattatgtt aatggatagc actgatccta aaatgggaga
    11701 ggcaaatcac aaaaacaagg gaatgcaagg ttgcccttcg aatttcaatg aatgcgtgaa
    11761 agattaccgg tgcctatgtt gaaatcaagt tggtgggagg gtagctggtt tgtgtggcct
    11821 ctgatggcct tcaccattct gaccgcatgc ctgggaaagt ttgaaacgaa acccacattg
    11881 accgacccgt gtcgcctcga ttggcaaaag caagcaggca tagcgaaagc ggatcttcct
    11941 gattcaactg tgaaccgtgg gctcccggtc ttggtattta atattcatga ccgccttctt
    12001 tccgattcgt tcttctcgag cgaggcccac actgaagggg aggtcgcatg cattggggat
    12061 ctagcctcca tgttcgatct tgtgctcttt caagaagcat ttgtccgtcc ggcgcaactc
    12121 gcccactaca ccaaacatgc ttgggcaaat cacccattgt tcactgaagg cggtggtggt
    12181 gactggtggc ccttgcgagc catgtgcaac atatgtttga gtcccggact cctgatgctc
    12241 gcccgtgaac agcctgagtc tatctattca gaaccctatg aggactttgc aggctggaat
    12301 actgacctga acaaagccga tgatttcttt tcgaagggct ttcagcttgt caaattcaaa
    12361 aaattttggg tgctgaatag tcatatggac gcgggtcgcg gagaggccag cattgaggcc
    12421 agagcccttc agtttagaca gattagtgcg ggcattaggc gtttggtttc tcctgatgca
    12481 cccctgctcg tcggaatgga cgcgaatctt cgcccggaca aaggggaacg cgacgcagag
    12541 attttggaag aatttctaaa tgccaatggg ctaaccctgg tgcaacaaag cgggccggat
    12601 ttgattgccg tcagaggaat ccgcaccgac cgccccaaat ccctaccatt aaaaggcatc
    12661 cttagcgatc acaacgccct gtccgtcatt atgtacccgc catccaggca agagtcacag
    12721 aaccaatgag gttctcatca atgttccggc aacttcccgt tcctttcagg tcaagtcgtg
    12781 ggacctttag cttgtggatc ggttttgagg agaactctat tttttaaagg actgtgaatg
    12841 gacatatcaa agatatcagc ccttctagtg atgcctcaac acacctgcca cctctacgag
    12901 acatctacag cgatttcaaa cacatgagga ggggcctata cggaaggtcg gagataaaac
    12961 ccctcaaaga atagagagga actcgcgctc ttattgaaag gcccgatcat acctccccaa
    13021 gttgctcaat atcctgaatc gggtaaaatt gccaaacggg ccaaagaaga tcaactagtc
    13081 aatcgacaaa tttatatttt tgtgttgatg tacacgttta ctctagtatc gaaatcattc
    13141 cccagagaat cgaacatttt ttcatccagg ccgatgaaac ttgcatcgtt ccgaagattt
    13201 tcatccgcta aagtccctcg gtcaacgata atgattttgg caagagacga ttctacctct
    13261 tcgatagttt gagacctttc cgaccttcca tgccagggca cttgcatcag gaaggcggta
    13321 taggcatcaa tattttcaaa tcggcttgtc gaggcaattg gaccactaat attgagggta
    13381 ctcaattttt cttgtagaac tttggccgcc tggtaactgg gcctttgagg gaaaaatgaa
    13441 ctgttggcga ggggaaagag aaaagataat gatataatgg ccagtgccat gaaatgaaga
    13501 atttttgcct ggctggtccg actcttggcc agatgcccca catagcccaa actcccgcca
    13561 agaagaaacg ggtaaacgga gtaataatat cgtttttctc ctgcataacc cggcaaaaat
    13621 atcaccacaa gacccccgat gaatatcgcc gcccaacgcc agcgatctga ccggatattc
    13681 tgtctccatg gagcatgaaa caagaagcca aatacgaccg agaccagacc gatgccaagc
    13741 cagtcaaagt ccttcagcag atgtatagtt ttcatcacat ttttatgaac aattttcaat
    13801 tggtgacgga gattctctgt gctctcaagc ggggaccagt gggaatactt ttttgagatt
    13861 ggatcctccc aggatgaaac tcgaccctgt tcaggaataa caaagtcaga aaaagtggga
    13921 tgtccccgcc caacaccagg cggccccatt acggcatgac taattcctgc acttgtcgaa
    13981 aacacaggct ttccatattt gagggacaat atgagaatcc aaggcccggc gataagcatt
    14041 aatccgatga tggtgatcgc gacgctacgt agagcacgag tcagactagt ctcttgagaa
    14101 acagtccata acaaccctgc cgccataatc agaataaatg caaatggcaa cccaggcgct
    14161 ttcgctaaat aggcagcccc gaataatagc ccggcagaaa attgaacgga tgccttgtcg
    14221 caccaatccg gctgcagagt tcggctaatg gccatgcaaa gtattccact cattaggaga
    14281 tccggagtga taccttgaac cgcccagtgg acactcataa gcgcaacgat cccgagagcc
    14341 acaaggatcg cggacgaccc gatccccaaa acccggaaga tcgccataca gcccatgaga
    14401 tacacaatcg cggatattcc catggccatc tgcgcagcgg ataacggttc ttccatcaag
    14461 ccaagcaacg gaataatgat ccagctcaac atgggccccc aataaccgga aaccgctaaa
    14521 tcgaactgtc ccgacgcata atattgcgca attcgaatat aggaaatccc atcagtactg
    14581 atccaatcct gattgaccca accggcccaa atcagcaacc caatctgaag aacaaagcag
    14641 ccgatcatgg ctctggggat gccttgtcga ttcatctcat ccattggaga taccccttca
    14701 cagaaagttc cctcgagaaa taagccgttt ccctaggcaa acttcaacct attttttttc
    14761 caattcatgt acctactatt ccactttact atctcaccac tttcatttcc gcctcacaca
    14821 tattcacaat caggactccg gatgcctccg aaccctggga aggcgctgtg tcacaaaagg
    14881 catacatgtt cgaggccggg aatttcctcg catttggatc cctttcataa aagatcctcc
    14941 acaatttcaa ctggcatttt ggcagagtcg tcctcgtgct cggaagaatt tttggattgt
    15001 gaagggagca tcccgtgcat gccagtatat tttcttcggg aggcattgca cgctcaaacg
    15061 gctttaaatt tcctcaaccc atgatatggt cacttgggcc atacaatttc gaataattct
    15121 tcagtatggt tacataagac tgaaggggac tttggtggtt gccttacacc atcgcctaac
    15181 gaaaatagac ccacattcct cagcctcggg tgttctgtat tcatttggcc agtcaggtga
    15241 cggcacttac tattcaccaa cagtccaaag aggtggaatg atggctgggt taacattaaa
    15301 caactcatac aaaaacggga tttcagaaaa cataaggagg agaaagagca atgtgctcaa
    15361 tcctacatac acgacaaatc cgccttcctt gtataatttt tctggatttt gaacagggct
    15421 atccttaagc atgccaatct taagataata cgtcaaaaat cccgccccaa taggaacaaa
    15481 aagtatcaac tccatatgat atcggacaag aaatattccg gatacaaatg aacaagccat
    15541 cgcatagaaa aacatactga gcaatagacg gttttctgtg taatatccga atgacttccg
    15601 atacgccttt gcacgtatcg gatcatcaat acgccgatat tcggcaaatc gcttggttgc
    15661 catgaaaaat gcgccaacca tccagtacgc caacacaagc gaaaggggag ggatgctatg
    15721 ggttacaagc gcaaaccacc cgagcaacaa ccgaatcgga ttattcagtg attcagtaag
    15781 cacatctaaa aaagggagat ctttggtccg tatgggggga atattgtaaa tgaggccgct
    15841 caccaaaaat accagggctg tcgtcccaaa atactcattt agggaaaaag caagaacgat
    15901 ccctgaagtc cccaataaaa accattctat ccaggcccaa ctggttttga ccttcccgga
    15961 aggaacgggc cgatcctttt ttaccggatg aagacggtca taaggcgcat caagaacttc
    16021 gttcaccaca taattgcttg aggcaatcaa acaggtcgct gcgatagcca ggagcaaagg
    16081 aaaaacactt tcccaggcga agagttccag ctcataaaac aaggccagca cgatgcccag
    16141 aatcataaag acattcttaa accagtgatc gatccgggca atctgaacat aaggccagag
    16201 tatccgaaaa aaactagatg gctgagtgga taacatcgca aagacctcta aaattagtaa
    16261 tcgaacgaag ttcggttgtt gcactcgcgt taaacccgcg agacgctctg cgagaaaata
    16321 tgacgtatgg cattccggca gctttggcac cacaggcatc gacgtcatgc cgatcaccga
    16381 catacaacac ctcccctgga tcaagattcc agagatgaca ggcataaaga aaccccttgg
    16441 ggtttggttt aaacgcatta atctccggat ccgttccgca taattccact gttaacggaa
    16501 cagaaatccc taactcctgc aatttttctc ttacagggta atcagaaaag actcccgtac
    16561 gaagacccat ttcgtccgcc aactgaaaaa attctaccat tccacggcgc cggcagatct
    16621 tgaggtattt caaaggacga agatacatcc attcccttac aattaattcc acctctctag
    16681 aggtcacaca gaatcgttcg gcaacaaggg aatattgtaa gctcgccaag gggatactgg
    16741 gatctttcat agcccgcaac gcttcacgtc cttctcgaaa acatttcaaa atcctccaga
    16801 cacccagagc ctctggaata gaacctaacg aaaatggagc agtagccagc tcaaaggcca
    16861 tcaggccacg aagggccttt tggtgataca gtgtcccatc aatatcgagc aaaacggcac
    16921 gaatctgctg aacctgtttc acaacccagg ctactcctca aaatcagaaa aatggctatt
    16981 caaaaataga ctctaaaaaa gctcacgagc actaaatgca tggtgcatgg tacaaattac
    17041 ttaaatccat caagatattg aatgcttgta caaaaatccg cttttaggtt ctcttaagaa
    17101 cttcctggtc catacaagca agaattggtc cgtaatgaat ctatatcggt agccagaatt
    17161 actggctgta caaaaatttt gaaaaacgga ccagtttcag gctgtatccg atagtagccg
    17221 tccagcttac gcgcaggtat caagaagcca agattgagaa ctctaagcgc ggtctactgt
    17281 cctcagttga ctcttcctat gatacatgct gttttctgac gacagtgaag gtgatagttg
    17341 ggactatttt catggccatc atcccctatt aggggaagac cgtttctgaa caactaccgg
    17401 cgggggaaga cgattaatct cccaagtagt tatccgtctt ttttgccttt tttatctcct
    17461 cctaaatgaa gccaaatgtc taatgaaaaa accatagtct ttggcctttt taggggattg
    17521 gcgagggcta tctggataat aatacatgat ttaatccaca ctgcaagcaa tcattcccat
    17581 taggaagggg aggcgagatt atggtccaat actcaaggac tggctgggca acgcggttgg
    17641 gtgtcatgag gcaatatcat aggatcgatt ataggcagga tgaatttgcc aaggacacca
    17701 ctcatatcca tggactggga aggagtctcg tcaatattcg cctggctaaa tataaaataa
    17761 cgcattgcca tcatccccat ttttttgcgc ttgtatcttg agaaataaaa caaatagcgc
    17821 aacaagcatg gctgccaaaa cctatacaaa cttttaagca aggtgtttat ttaagatctt
    17881 ctaagcatgt cctagatttt ttgcccacac gggaagccaa gatcacttta aaatactggg
    17941 aattccattg gtctcttgag aaagaaaagc ctaaatggtc gaatggctcc ctgggttacc
    18001 aacaaccaat gatttttgag acggcgaatt cccggacaat acaggctctg ctccctttca
    18061 gggaaatacc cgcagatgca aatgatacaa tgatatctgt ataacccgtt ctatcctagg
    18121 gtgcaagtat gattgcagcc atcttttggg aagcccctat cgcctccccc cttatggagc
    18181 agttaacccg tgaccaccct ctaccatata ccgaccctta aacaaaggcg cgaggttttt
    18241 gagctcgtcc ccttctcgat agagccgtct acgcactc
//
LOCUS       DAORAQ010000044        18222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_542_length_18222_cov_68.164639, whole genome shotgun sequence.
ACCESSION   DAORAQ010000044 DAORAQ010000000
VERSION     DAORAQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 18222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18222
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_542_length_18222_cov_68.164639"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /gene="flgB"
                     /locus_tag="PKM72_06110"
     CDS             complement(<1..343)
                     /gene="flgB"
                     /locus_tag="PKM72_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNP60394.1"
                     /translation="MMISPWDEGTRLFERSLDVRSGIQETIASNMANEETPGYKSRIL
                     PFQETFNAVLRGDGPLESVVTNPKHVRIGSEDSRIFQHTIKQDAGAGLDENTVNLEKE
                     MTLMAENTLMYM"
     gene            complement(669..2057)
                     /locus_tag="PKM72_06115"
     CDS             complement(669..2057)
                     /locus_tag="PKM72_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP60395.1"
                     /translation="MIDTEGGASRGILVVEDDEQLQSALTHALSQHGYAVTVARDGYE
                     GLERMEREAPWLVLTDLNLPGKGGMEFLREVRSQGYHMPVVVMTAYGGVDAAVEALKY
                     GATDFLQKPFPLDRLEKLIDQLKYEQPVSGLDVLREQERNRREEPREGFLTRDPKVLQ
                     TISMLEMVAGSQATILIQGESGTGKEVLARHVHRHSPRASQPFVAINCAALPEGLLES
                     ELFGYEKGAFTGALARRCGKFELAHQGTLLLDEIGEMSLGLQAKLLRVLQEREVDRLG
                     SRQPVQVDVRVIATTNRNLAQEVQAGRFREDVYYRLSVMPFTIPPLRDRPEDVQLLAE
                     YFANRSLRRNRRTPCEISEEAMSHLKRRPWRGNVRELENVIERGVLLAGNGPLRMEHF
                     QFEEPVLTANHASAPSGTIWEMERELILKTLERHDGNRTHAARTLGISIRTLRNKLRE
                     YRQLNGGLSLSI"
     gene            complement(2069..3232)
                     /locus_tag="PKM72_06120"
     CDS             complement(2069..3232)
                     /locus_tag="PKM72_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60396.1"
                     /translation="MSQLNPKPMIALSSKKNGDTSWPTLDPITEPTISSETLSLALEA
                     KYAETQRLHHHLHRLVKSLPLPALLYNRKGRIVTANHKAQILMGEIDWKKMNWHIQDL
                     WTSLVWPTVPFTDWKWKAGRVSCWDCPLGGGDQPSSLTVRYLRYEEEVLSEGTEQLQR
                     LAAIGEQVGRLAHDIRNPLASIEWFATLLGRDRQSGLERGDLAGQLLQAIRSLDGLVS
                     NLLTASTPLKRERQSVNLSRLLDDVELLAMYSFRRKRLTIHREKEVSHLEILGHEQLL
                     KQALLNLLLNAIQASPQDGHIEIRCRLMSLPVTGEPVSPGINGVALSIRDEGCGMSKE
                     ELSRVFQPFYSKRQGGTGLGLPIVKDIVQVHQGRIEIESQTDKGTTVKLFLPQ"
     gene            complement(3213..5186)
                     /locus_tag="PKM72_06125"
     CDS             complement(3213..5186)
                     /locus_tag="PKM72_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP60397.1"
                     /translation="MPEMIQHGQDAMRAGQLLEARDMFATYLNEQEDGKFAEGVRWVM
                     ASLPDPSDEPGKENFQRIKRLQAMKTSHPESVYAPWALCAMGEVYWEAGWFSEASGIF
                     EEFLRSYPEHPLAGGVMVEAGLGYLSNKQYLEAALIFRRVVEEPKWNAHRIKGALGLA
                     DATAKAKAWKQAYYWYRVVEAERPELIRQSAESSYHYGLTEIAVGNPAMAISRFLLPV
                     NLHPHQEAAGRALNHISEKLRKEGHDFLALYFADHARQQFPDQEPGRRGQAALTRWIV
                     EFVSREHAKEDWNKVYQRLDDLEIYLSLSWDYVLETAGALSHAPEKDVADESLLWMGQ
                     AYQALEDFSDAVQTFTRLIIAASSDVKRLEGQERLASLLQDQIRWFYARKAWVPLLKF
                     HADHRDAFQLVPLERERLLMVAQAYQQVNLPAEALHWYDQLIKEYPNSPHREEILLQQ
                     VLVAQEQGNGSLVREAGASYLQEFPEGRGRGQVEIALGMEAFADKRYVEAIRNFTSAL
                     QHVDGEVGQRYLLRNRARANRALGQMELVVQDMRQAVSIEPAQASDVLRLGDAMFDQG
                     DYVEAQSLYDQVLALDVPAALHTWAKYRLAVSFEHMGKSAEAASLLAEIKELKTRSPE
                     LEHTIRSAATAVLDEMSLNETPSTRIPDVPIKS"
     gene            complement(5529..6989)
                     /locus_tag="PKM72_06130"
     CDS             complement(5529..6989)
                     /locus_tag="PKM72_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP60398.1"
                     /translation="MDVFDSAESGITKQVARILLVEDEVDVRESLKLQLEDEGHQVVD
                     TESGSDALALAESSLFDIVLTDVMIPDINGIELVQRIMQLPNHPVTVVMTGYGSVEMA
                     VKAIKAGAFDFLSKPFSMDVLSATVASALRVKCLQDENVELKKTVKGQFSLGKLISSS
                     QVMQEVFQLIDCVADTDSTVLILGESGTGKELIAQTIHCHSTRHRGSLIPVNCGAIPE
                     ALLESELFGHEKGAFTGAVASRVGRFELATGGTIFLDEIGDLSPPLQVKLLRVLQERT
                     FERVGGSRTYKSDARVIAATNQDLETLVAAKQFREDLFYRLNVVPVLVPPLRQRVEDI
                     PLLLQHFITMFNDRRKAELTGVAEDAMSLLCDYPWPGNVRELGNIVERIAILKRRGLI
                     VPGDLPEKIRRLPPSHLPTMPAAIPVAGMDLSRTVEEFENRLILEALERTNWVKSKAA
                     RLLQINRTTLIEKLKKKSLAEVVAQRAAPSEDLADV"
     gene            7282..7509
                     /locus_tag="PKM72_06135"
     CDS             7282..7509
                     /locus_tag="PKM72_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60399.1"
                     /translation="MDTPHIPQPAPMPIFMLFDTQTGYLYGHLFATGPEDPFLQEVID
                     WWTHYCSNTPIGVDYPGVTKHPQMASNLSSN"
     gene            complement(7510..8118)
                     /locus_tag="PKM72_06140"
     CDS             complement(7510..8118)
                     /locus_tag="PKM72_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60400.1"
                     /translation="MMMLWGLAGWPLHVWSQEVVTDQVTITSARGQLFGITSGEGIAK
                     IVLASGEDVLVVETKGVTGYVQTTTRLLGFSGRLQRWVELNLSSAEEEVTATVTPRMI
                     IVQGQQAAYGFQSDLGRWKRNSWGAGELLVESIVNDHVAVLITNRRALGFSALTGGFF
                     PQDLPSGNAIVQTEANANVVVIHLSGLMLVFRSGLAIWTVLP"
     gene            complement(8160..10457)
                     /gene="lptD"
                     /locus_tag="PKM72_06145"
     CDS             complement(8160..10457)
                     /gene="lptD"
                     /locus_tag="PKM72_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNP60401.1"
                     /translation="MTVAGLGGDRRVLSRRSHGLLVGVFILLAMSATGETAFGQADSD
                     PVESEGLSADTSSQEEPAESTSEGSPVAINADRIEYSRDREEYHAIGAVDVRRGPIHL
                     NSDEATLQRLTGRLTARGNVHLQDAQTDIWSETLQLNLNTEAGVITNGQIFWKDQNSF
                     VTGRRLQRFSETHYRVKEGSFTNCDVQNGEIPAWRFKFQDMDLEYEDSLFGKGVWFNV
                     NDVPVIPLPTFRYPLGASRKSGFLFPTTGYNTNFGYQYRQGYFWAINPSSDLTVSPQI
                     LTERGGGADLQYRYVWNRLTKGEWLIRSLYDTEQSRGRAEVRGAHVQQFNPDLSLRMR
                     ANFSSDRTVLQNFSSSGTQRALPSQESNLTILQRLDHGALYFSGQYIQPLATGGNSTF
                     QRLPEVGHRFVNPSVLGSPISLTAETTAVQFYRQTGFNVGRLDVLPGLSMEGLHLGHT
                     LGFRPQVKFREVGYTRGITEKSFQHRETFWVGAELFSNMSKRFSIGEGDWIRHSIQPN
                     VIYEYVPQTKQAEFVQIDAVDDLIQKSLVTYSLKNRLSQIGGTESNSWLDLLFAQSYH
                     VANPSPLASKFSDIWTRGQFHSPVNSSAFISAFDLRVDAFYDPNRKYFSQMNTDVFIQ
                     SRQAWYVTVGQRYAREGSRVRRGDIWNPVSFNEVLAPAQEILYLTTGGGVRLPYGVTL
                     GTKWYHDFRTGETAELDVVGLYQNPCRCFSLGLTYTKLPDRSQIEWLVSLTGLWGTQG
                     HGTQLMKLILGPIMGGERGVPWDYR"
     gene            complement(10454..11743)
                     /locus_tag="PKM72_06150"
     CDS             complement(10454..11743)
                     /locus_tag="PKM72_06150"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNP60402.1"
                     /translation="MATYLETLEFLFSLHRFGIKLGLESITDILDRLDNPQHRYATVH
                     VAGTNGKGSSSAMVAAILQASGYRVGLYTSPHLIDFRERIRVQGADISEEAVCGLIAQ
                     IRHAANSLTTLTFFEFTTALAFLHFRNEQVDIAVVEVGMGGRFDATNVLNPTGVLITG
                     ISFDHEAYLGDSLQKIAQEKAGVIKRKVPIVLGPMPEGIAQIFETQARKWSAPIFRMG
                     KEFSFLETSATTFTYQGLGWTIPEIQANLLGRHQMVNATNALAVLETGVDGTFPVSQT
                     AIRIGLQSVKWRGRLEVVDRNPLLIVDGAHNLAGAQVLFDFLYSQIHDGAGRKLILVV
                     GMMRDKNLQGFLRVFLPLVDHLILTQPRMERAASVDELKHVVDREDLVPCLLADSWEA
                     VCQAKEMANPSDVICVTGSLFLVGEVLNRLTQPGSPT"
     gene            complement(11890..12750)
                     /gene="accD"
                     /locus_tag="PKM72_06155"
     CDS             complement(11890..12750)
                     /gene="accD"
                     /locus_tag="PKM72_06155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNP60403.1"
                     /translation="MGWFKRNKNTDTEGTSIKIVEGMWVKCAVCRTIIFKREVERNLK
                     VCPKCQYHFPLSVGERLELIVDPDSFEEWDGELVPGDPLQFIDTMPYRKRIQKAQEKT
                     GKKEAILTGRCQIGGKPAALGIFDFSFMGGSMGSVVGEKICRAIDQALIGHMPFLLVT
                     TSGGARMQEGTLSLMQMAKTASALAKLQEARLPFVVLLTDPTFGGVTASVAMLGDIIL
                     AEPKALIGFAGPRVIEQTIKEHLPEGFQRAEFLLEHGMVDHIVERKILKATLETLLAH
                     CVTTVSSSSV"
     gene            complement(13015..14076)
                     /gene="moaA"
                     /locus_tag="PKM72_06160"
     CDS             complement(13015..14076)
                     /gene="moaA"
                     /locus_tag="PKM72_06160"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNP60404.1"
                     /translation="MEAGEAVALPDAVRDSLGRPLRSLRVSVTDRCNLRCQYCMPEEN
                     YVWLPRENLLTFEEIALLTNLFSDQGIDRVRITGGEPLLRNNVAELIRMLRQNSRIQD
                     IAMTTNGVLLSEQAQALFDAGLHRVTVSLDTLKPERFKALTRRDTLDRVFEGIASVGR
                     VGFTGLKLDTVAMKGYNEDEVLPLIEYGKQVNGEVRFIEYMDVGGANDWSADKVLSRK
                     EILKMVGDHYGKVEPVEEVSSAPAQRFRLPDGTTFGIISSTTVPFCSTCDRSRLTADG
                     MWYLCLYAKTGLDLRKLLRMERPREDILSIIQSVWESRKDRGAEERKELEQVSGRGRY
                     IDIERLRQDPHLEMHARGG"
     gene            complement(14076..14519)
                     /locus_tag="PKM72_06165"
     CDS             complement(14076..14519)
                     /locus_tag="PKM72_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNP60405.1"
                     /translation="MAVDLVESMLVRVQLENFSIDEELDRVRSRSRRIGGIAMFLGTA
                     RDHSKGRDVSSMSFDHYEGMAQKKLKEIREQALRDFDIIEALVLHRYGPIEIGENIVL
                     IIVGAEHRADAFKACQWCIDELKKITPIWKMETTPEGEVWVEEHP"
     gene            complement(14524..14802)
                     /locus_tag="PKM72_06170"
     CDS             complement(14524..14802)
                     /locus_tag="PKM72_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNP60406.1"
                     /translation="MKVKLFGMLKTMIPGGKDLEVAIAEGGQVRDLVHSIQVEHPQVG
                     ELLDKKKVLISVNQEIAHWDTVLKEADEIALLPPFAGGSCVSWAKEKG"
     gene            complement(14852..15355)
                     /gene="moaC"
                     /locus_tag="PKM72_06175"
     CDS             complement(14852..15355)
                     /gene="moaC"
                     /locus_tag="PKM72_06175"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNP60407.1"
                     /translation="MANLTHFNESGRARMVDVTDKRSTERVAVAQGKVFILPETLEKI
                     HEGRIAKGDVLAVAQVAGVMGAKRTPDLIPMCHPLLLTGVDVDFKESSQPNSDGKYAI
                     TIVATVKTTGNTGVEMEAMTAVSVAALTIYDMCKAVDKGMEFGEIALVSKSGGKSGTY
                     VRVQKTK"
     gene            15520..15873
                     /locus_tag="PKM72_06180"
     CDS             15520..15873
                     /locus_tag="PKM72_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNP60408.1"
                     /translation="MDTTTIIANPTLANKKLGLLLSTSPEHPNAETVYHLAKTALANK
                     VDTYLYFIDEGVKNLEDRRFSELAKDGLKLFVCAYGCQQHHISTDGYGKEVTFCGLVI
                     LSNIIDGCDRFLAFN"
     gene            16094..>18222
                     /gene="shc"
                     /locus_tag="PKM72_06185"
     CDS             16094..>18222
                     /gene="shc"
                     /locus_tag="PKM72_06185"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNP60409.1"
                     /translation="MLTSTRYLILETAIDLLGRDGASALTLEHVAREAGLSKGGLLYH
                     FEGKEQLLTGLIELLIQDLDRKQVGNFLSSSIDEELPWPAIETDIVENPISHRSLQGH
                     EIASILMAALAINPNLLDPIRERYQNWQSIFLANTVDPTRSLVGRLATEGLWFRDAMG
                     LAPPTREQRAALVLEIQSQTQSGELMREPSASIIPTQSTDAFAELASFKANVEELEQQ
                     AYQTTQVSARSLLAKQYSQGFWQGDLTADTTLESDYVLLLLWLYPPENGVWEPRIQTK
                     VKEAIRTILDRQLSDGGWNIYSEGPAEVNATTRAYVALRVGGHDPDQPLMQRARECIL
                     ALGGLQATNSYTKNNLSMFGLFPRKYTPSVPPELVLIPGNVLYEISSWSRAIVVPLSI
                     LQASGAQRRVPGNWTVDELMVPNRKLRFPKKDPFSLMFHQVDKILKIWEKRGFKDIRA
                     KAIREAEKWMLDHMRFTDGLGAIFPGMMYALMAMDALGYERDHPDFIETLGQLEGLIL
                     EKEGALQFQPSLSPVWDTAISMFALGELGVGEPQAMQRAADWLLSKEVRRKGDWSVKR
                     PDLQPGGWPFQFANELYPDIDDTAMVLLALEHAKASDPDRQTRAEGRAINWLLGMQSS
                     DGGWAAFDVDNNWALLNKVPFADHNAMLDPSCPDITGRVLEALCRRDYTHQHPAIARA
                     VQFLLGHQQANGSWDGRWGVNYTYGTFL"
BASE COUNT         4620 a         5128 c         4082 g         4392 t
ORIGIN      
        1 ccatatacat caaggtattt tccgccatca gggtcatttc tttttccaaa ttcacagtat
       61 tttcgtctaa tccggctccc gcatcctgtt taatggtgtg ctgaaatatc cgggaatcct
      121 ctgagccaat gcgcacatgc ttcggattgg tgacgactga ttccaatgga ccatcccccc
      181 gaagaaccgc attaaacgtt tcctgaaaag gcaggatcct ggacttgtag ccaggtgttt
      241 cttcgttggc catgttggag gcaatcgttt cctgaatccc gctccggaca tccagtgaac
      301 gctcgaaaag tctcgtacct tcatcccaag gggaaatcat catggcagtt tctcctctcc
      361 ggttcccatg ttctcctgtg tcttcacctg tatcccttcc tccccgacca tggaatcctt
      421 gacgtcgtca ttcaagtcag caacaccaat gccagaaatc atccgtaagg aatccacatg
      481 aaggagaaaa aagagttcct cacgttttcc ccgtcacgtt ttccccctta cccctacttc
      541 tcacgcttta catcttgtct ggtctggagg aaacttttgc ctgcaccggc atatttttcc
      601 gctcaactcc tgtcattcct cataacagat cgaagcgaga ctctctgatg ttctccgact
      661 gcctcaagtt atatactcaa cgaaagacca ccgtttaact gtcgatattc ccgcaattta
      721 ttccgtaatg tacgaatgct gattcccagg gtcctggcgg catgggttcg attgccatca
      781 tggcgctcca gggtttttaa aataagttcc cgttccattt cccagatagt tccacttggc
      841 gcactggcat gattggccgt caacacgggt tcctcaaact gaaaatgctc catccgtaaa
      901 gggccgttac cggctaacaa cacgcctcgc tcaatcacat tctccagttc ccgcacgttt
      961 ccccgccacg gcctccgctt caaatgcgac atcgcctctt cagaaatctc acacggtgta
     1021 cgccggtttc gccgcaatga ccggttggca aagtattccg ccaacaattg aacgtcctca
     1081 ggtcgatcac gcaacggggg gatagtgaac ggcatcacac tcagccggta atacacatcc
     1141 tcacgaaagc gcccggcctg aacttcctgc gccagattcc gattcgtggt ggcaatcacc
     1201 cgcacatcca cctgcaccgg ctgccgtgag ccaagacgat ccacttcacg ttcctgaagg
     1261 accctgagta atttggcttg taaccccaaa ctcatttccc cgatttcatc caacaacaag
     1321 gtcccctggt gggctagttc aaacttgccg catcgtcttg cgagggcacc ggtaaaggct
     1381 cctttctcat agccgaataa ttcactttcc aagagtcctt ccggcagagc ggcgcaattg
     1441 atggccacga agggttggga ggcacgtggg ctatgacgat ggacatgccg cgccagaact
     1501 tcttttcccg tcccgctttc cccttgaatc aaaattgtcg cctggctccc tgccaccatt
     1561 tccaacatac tgatcgtctg cagcactttc ggatctctgg tcagaaatcc ttcacgcggt
     1621 tcctcacggc ggtttctttc ctgttccctc aagacatcca gtcctgagac aggctgctca
     1681 tactttaact ggtcaattaa tttttcaaga cgatccaggg gaaacggctt ctgaaggaaa
     1741 tcggtggccc catatttgag agcctcaacg gctgcatcca ctccaccata tgccgtcatg
     1801 acgaccacag gcatatggta gccctggctt cgaacctcac gtaaaaattc cattccgcct
     1861 tttcccggca aattcagatc agtcaacacc agccatgggg cttcgcgttc cattcgctcc
     1921 aacccctcat agccatctct ggcaaccgta acggcatacc catgttggct taatgcgtga
     1981 gtcagggctg attgcagttg ctcatcatct tcaacgacca ggatcccccg gctcgctcca
     2041 ccctcagtgt ctatcatgcc aatctccttt attggggaag aaatagtttg accgttgttc
     2101 ctttgtccgt ctggctttct atctcaattc tgccttgatg cacctggact atgtctttca
     2161 caatcggaag cccaagaccg gtcccaccct gacgcttcga ataaaatggc tgaaatactc
     2221 tggagagttc ttctttggac atcccacatc cttcgtctcg aatacttaat gccacgccat
     2281 tgattccagg agagacgggt tctccagtaa ccggcaggga catcagccga caacgaattt
     2341 caatatgacc gtcttgcggg gaggcctgaa tcgcattcag gagcagattc aaaagggcct
     2401 gctttaataa ctgctcatgc cccaagattt cgagatgtga cacttccttc tcccgatgga
     2461 tagtcagccg ttttcttctg aaggaataca tggctaggag ctccacatca tccaacagcc
     2521 tcgagaggtt cacactctgc cgttcccgct tcaatggcgt gctcgccgtt aagagattcg
     2581 agaccagccc atccaatgaa cgaatggcct gaagtaattg acccgccagg tccccgcgtt
     2641 ccaaaccgga ctgacggtcg cggcccaata gtgtggcaaa ccattcaata cttgcgagcg
     2701 gattgcgaat atcatgcgcc agcctcccga cctgttctcc tatggccgcc aagcgttgaa
     2761 gctgctccgt cccttcactg agcacctcct cttcgtatct gagataacga acagtcaaac
     2821 tggagggttg atcccccccc cctaacggac aatcccaaca ggacacccgg cccgccttcc
     2881 atttccaatc ggtaaaggga accgtcggcc acaccaggga agtccagaga tcctgaatgt
     2941 gccaattcat cttcttccaa tcgatttctc ccatcaggat ttgagccttg tgattggcag
     3001 tgacaatccg gcctttccgg ttgtagagca aggcgggtaa cggaagactc ttcaccaaac
     3061 gatgaaggtg atgatgcagt cgttgcgttt ctgcatactt ggcctcaagc gccagactga
     3121 gggtttcact ggaaattgtc ggctccgtga tagggtcgag cgtcggccag gacgtatccc
     3181 cgtttttttt ggaactgagc gcgatcatcg gtttaggatt taattgggac atcgggtatc
     3241 ctggttgatg gcgtttcatt caatgacatt tcatccaaca ccgccgtggc ggctgaacga
     3301 atcgtatgtt ccaattccgg agaacgggtt tttaattcct tgatttcggc aagtagtgac
     3361 gcggcttcag cggatttgcc catatgttca aacgagaccg ccaaccgata cttagcccag
     3421 gtgtgcaacg ccgctggcac atccaaggcc aagacttgat cgtacaggct ctgggcttcg
     3481 acatagtcac cttgatcaaa catggcatcc cccaagcgga gaacatcgga agcctgagcg
     3541 ggttcgatag acaccgcctg tcgcatgtct tgaaccacta actccatctg cccgagggct
     3601 cgattggccc gtgcccgatt cctgagcaaa tagcgttgtc ccacttctcc atcgacatgc
     3661 tgtagggccg acgtgaaatt ccggatcgct tctacatacc gtttgtcagc gaaggcttcc
     3721 atgccgaggg cgatctccac ttgccctcgt cctcggccct cgggaaactc ctgtaggtag
     3781 gatgcccccg cctctcgaac caatgaaccg ttgccttgct cctgagcgac cagaacctgc
     3841 tgaagaagaa tctcttcccg gtgcggactg ttggggtact cctttatgag ttggtcatac
     3901 caatgcaagg cttctgcagg aagattcacc tgctgatagg cttgcgccac catcaagagt
     3961 cgctcccgct ccaatggcac cagctgaaac gcgtcccgat ggtcggcgtg aaacttgagt
     4021 aatggtaccc aggctttccg agcatagaac cagcgaatct gatcctgtaa caaggacgcg
     4081 agtcgttcct gcccctccag acgttttaca tccgacgagg ccgcgataat gagacgggtg
     4141 aaagtctgaa cggcatcaga aaaatcctct aacgcctgat acgcttgccc catccacagc
     4201 aggctttcat cagccacgtc tttttcaggt gcatgactta acgcacccgc ggtttccaga
     4261 acataatccc atgagagcga gaggtatatt tccaaatcat ctaaccgttg gtagaccttg
     4321 ttccagtctt ccttagcatg ctcccgggaa acaaactcca cgatccatct ggtgagtgca
     4381 gcctgcccgc gtcgaccggg ctcctgatcc ggaaattgtt gtcgggcatg atccgcaaaa
     4441 tacaaggcta aaaaatcatg cccttccttt cgtaattttt ctgatatgtg gttgagggct
     4501 ctcccggctg cttcctgatg cggatgaaga ttgactggca ataaaaatct ggatatcgcc
     4561 atggcgggat ttcctacagc aatttctgtc aagccatagt gatacgatga ctccgcagac
     4621 tggcgaatga gttcaggcct ttcggcttcc acaacccgat accaatagta ggcctgcttc
     4681 caagcctttg ccttggctgt ggcatcggcc agccctaatg cacctttaat gcgatgggca
     4741 ttccacttgg gttcttctac cacccgccgg aagatcagcg ctgcctccaa atattgttta
     4801 ttactcagat accctagtcc ggcctcaacc atgactcctc ccgctaatgg atgttctgga
     4861 taggaccgga ggaattcttc gaatatcccg ctggcttctg aaaaccatcc agcctcccaa
     4921 tacacttccc ccatggcaca aagagcccat ggagcataca cactttccgg atgacttgtt
     4981 ttcatggcct gtaaccgctt aattcgttga aaattttctt tgcctggttc atccgaagga
     5041 tcaggaagag aggccatgac ccaacgaacg ccttctgcaa actttccatc ttcctgttca
     5101 ttcagatagg tagcaaacat gtctcgggct tccaagagtt gtccggcccg catagcatcc
     5161 tgtccgtgtt gaatcatctc tggcatcacc atatccctgg aaacgtacca ggaaaattca
     5221 gaacaggggc ctcccataac cgtgctgggc cagataagac acgacagggc aatccaagta
     5281 aatttcctca ctttttgttt ctgctccaac ctgctgaacg tattcagcaa aaattagacc
     5341 ggagttattc ttgccggggg aaaattttac tcacagggtt tttgttgata aaccaggagg
     5401 tttgagacaa acaccgacga gcggacaaaa ttgccgccgt caaggagttg acgcaatggt
     5461 cagaacctca ggctggtttg gaaagggaaa gctagaacag ggtaggaaag gccaacggtc
     5521 ttgtatcgtt acacgtcagc cagatcttcc gatggcgcgg cccgttgtgc gaccacttcg
     5581 gccagagatt tcttttttaa cttttcaatg agagtggtgc gattaatttg caaaagacga
     5641 gccgccttac ttttgaccca attggttcgc tcaagagcct caagaatcag ccggttttca
     5701 aattcctcaa ctgttctgga aagatccatc ccggccaccg gaattgccgc cggcatggtg
     5761 gggagatggg aaggtgggag tcgcctgatt ttttccggta aatctcccgg gactattaat
     5821 ccccgccgtt ttaaaatagc aatccgctca acaatattcc cgagttcacg aacgtttccc
     5881 ggccagggat aatcacataa gagactcatc gcgtcctcag ccacaccggt gagttccgct
     5941 tttcgccgat cattgaacat cgtaatgaaa tgctggagta atagcgggat atcttccacg
     6001 cgttgacgga gaggaggaac aagaaccggc accacattta accgataaaa cagatcttct
     6061 cgaaattgtt tagccgcgac cagggtttca aggtcctgat tggtggcggc aatgacacgg
     6121 gcatccgact tataagttcg actacccccc actcgttcaa acgttcgctc ctgcaggacg
     6181 cgaagaagtt tgacctggag cggaggactc agatcaccga tttcatcgag gaaaattgtt
     6241 cctccagtcg ccagctcaaa tcgtcccact cgtgaggcca ccgctccggt aaatgcccct
     6301 ttttcatgac cgaacaattc actttccaat aacgcctcgg ggatcgctcc gcaattgaca
     6361 ggaatcaggc ttccccggtg ccgcgtgcta tgacaatgaa tggtttgggc aattaattct
     6421 ttgccggttc cactttcccc taaaatcagc acggtactat cggtgtccgc cacgcaatca
     6481 atcaactgaa acacctcttg catcacctga ctggagctaa tgagttttcc caaactaaat
     6541 tgccccttga cggttttctt cagttcgaca ttctcgtcct gaagacactt gacccttaag
     6601 gcactggcaa cagtggcgct caagacatcc atggaaaatg gtttagagag gaaatcaaag
     6661 gcgccggcct taatggcttt caccgccatc tctaccgacc catatcctgt catgacgacc
     6721 gtcacaggat gattcgggag ctgcataatg cgctgaacca actcaattcc gttgatgtcg
     6781 gggatcatca catcggtcaa gacaatatcg aacagggagg actcggcgag agccaaggca
     6841 tcgcttcctg attccgtgtc cacaacctga tgcccttcat cctcaagctg caacttgagg
     6901 gactcccgca catctacctc atcttcaacc agcaggatcc tggcaacctg cttcgtgata
     6961 cctgattcag ccgaatcaaa cacatccatg aatggcctcc ctctcaaccc atataaacgc
     7021 acatcccgtt gacccggttg tgcggtgaag gaacatagct accccatttg acggggaagg
     7081 tcaagggttg tcttctaatt tgccccaatc gttgctgagt cccctctccg atctccctga
     7141 aacccttgaa tcggccttga gtccaaaaag acggtcagag ccgtttgagc aaaaggccta
     7201 tttaagaaaa ataaaatacg ttccgataag aatacgtagg caaaaacaga aggaacctct
     7261 gaggatcggt ctcaactact catggacacg ccacatatcc cacagcctgc acccatgccg
     7321 attttcatgc tctttgatac ccaaacgggt tacctctatg gtcacctatt tgcgacaggg
     7381 ccggaagatc catttttgca ggaagtcatt gactggtgga cccactattg ttcgaacacc
     7441 cctattggag tggattaccc aggcgtgacc aaacacccgc aaatggcgag caatctttcc
     7501 tctaattaat caaggcaaca ccgtccaaat cgctaagccg gaccggaaaa ccaacatcaa
     7561 tcctgacagg tggatgacca cgacgttagc attggcttcc gtctggacaa tcgcattgcc
     7621 ggatggaagg tcttgcggga aaaatcctcc ggtcaacgca gaaaatccca atgcccgtct
     7681 gttggtaatg agaacagcca catggtcatt cacgatactc tccaccaaca actctccggc
     7741 tccccaagag tttcgtttcc aacgacccag atcactttga aaaccataag ccgcctgttg
     7801 cccttgaacg ataatcattc tgggagtgac cgtcgcagtg acctcctcct ccgccgagga
     7861 aaggttcaac tccacccacc gttgtaaacg acctgaaaac cccaacaacc ttgtggtggt
     7921 ctggacatat ccggtcacgc ctttggtttc aaccaccagc acgtcctctc cagaagctaa
     7981 tacgattttg gcaatgcctt cgccggaagt gataccaaac agttgtcccc ttgccgatgt
     8041 gatggtcacc tgatcggtga caacttcttg cgaccaaaca tgaagcggcc agccggccaa
     8101 tccccataac atcatcaccg gtacaatgca ccaatggaca ggcataatcc gcatgaccgt
     8161 taccggtagt cccagggaac tccacgctct ccgcccatga tggggcccaa aattaacttc
     8221 attaattgtg ttccatgccc ttgggtcccc cacaacccag tcaagctgac gagccattca
     8281 atttgagaac ggtcaggaag cttcgtatag gtgagcccaa gcgaaaaaca tcggcaagga
     8341 ttttggtaca atcccaccac atccaattca gccgtctccc ctgtccggaa gtcatgatac
     8401 catttggtgc ccaaggtcac accatacggc aaccgcactc cacccccggt cgtcaaatag
     8461 aggatttcct gggccggagc caatacttca ttaaacgaca cggggttcca aatatctccc
     8521 cgacggactc tcgacccttc tctcgcgtac cgttgcccaa cggtcacata ccacgcctgt
     8581 cgactttgaa tgaacacgtc ggtgttcatc tgagaaaaat atttcctgtt cggatcgtag
     8641 aaggcatcca ctctcagatc gaatgccgag atgaatgctg aggaattcac tggtgaatga
     8701 aattgtcccc gtgtccagat atccgaaaat ttggaggcca agggcgacgg attggccaca
     8761 tgataacttt gggcaaataa cagatccaac caggaattag attccgtgcc accaatttgg
     8821 ctcagacgat tcttcaagga ataggtgacc agactctttt gaatcaaatc atctaccgca
     8881 tcgatctgaa cgaattccgc ttgtttcgtc tgtggcacat actcataaat cacattcggt
     8941 tgaatagaat gtcggatcca atccccctcg ccgatggaaa accgtttcga catattcgaa
     9001 aataattcag cgcctaccca aaaggtttca cgatgttgga atgatttctc ggtgattccc
     9061 cgggtatagc ccacttcacg aaatttgact tgaggacgaa atcccaaggt gtgccccaga
     9121 tggagtcctt ccatcgaaag ccccggcaac acgtcgagcc gaccaacatt gaaccccgtc
     9181 tgcctgtaaa attgcaccgc ggtcgtttcc gctgtcagcg atatgggact gcccaagaca
     9241 ctgggattca cgaagcgatg cccgacttca ggtaaccgct gaaaagtcga attcccgccc
     9301 gtggctaacg gctgaatata ttgacccgaa aaatacagcg ctccatgatc aagtcgttgc
     9361 aaaatggtga gattcgattc ttgactgggt aaagcccgtt gagtcccgga actgctgaaa
     9421 ttttgcagaa ccgtgcgatc acttgaaaag ttggcccgca tgcgtaaaga caaatcggga
     9481 ttgaattgct gaacatgagc cccccgaact tcggctcgcc cacgactttg ctcggtatca
     9541 tagagcgagc gtatcaacca ttctcccttt gtgagccgat tccacacata gcgatattgc
     9601 agatccgcac ctcctccacg ctccgttaat atctgcgggg aaacggttaa atcgctactg
     9661 ggattaatgg cccagaaata cccctgccga tactggtacc cgaaattcgt attgtacccg
     9721 gttgtcggaa acaagaatcc gctttttcga gaagcgccaa gcggatatcg aaaggtggga
     9781 agcgggatca ccggcacatc gttcacattg aaccacacgc cttttccaaa caaactgtcc
     9841 tcatattcca aatccatatc ttgaaactta aatcgccatg ccggaatctc gccattctgg
     9901 acatcacaat tggtaaaaga gccctccttg acccgataat gggtttcgga aaaacgctga
     9961 agccgtcgcc ccgtgacgaa ggaattctgg tccttccaaa aaatttgtcc attggttatc
    10021 acacctgctt cggtattgag attgagctga agcgtttccg accagatatc cgtctgggca
    10081 tcctgtaaat gcacgtttcc gcgcgccgtt agacgccccg tgagtctttg aagagtggcc
    10141 tcatccgaat taagatgaat gggaccacgc ctaacatcca cggcaccgat agcgtgatat
    10201 tcttcccgat cacgactata ttcaatccgg tcggcattaa tggcgacggg ggatccctct
    10261 gaagttgatt cggcaggctc ctcctgagac gaggtatctg cggagagtcc ctcggattcc
    10321 acagggtcgg aatcggcttg tccgaacgca gtttctcccg ttgccgacat cgcaagaaga
    10381 atgaatacac cgaccaacaa cccatgagat cggcgagaga gaacacgccg gtctccaccc
    10441 aggcccgcaa cagtcacgtt ggggaacccg gctgagtgag ccgattgagt acctccccaa
    10501 ccaaaaataa cgaaccagtg acacaaatga cgtctgatgg attcgccatt tccttggctt
    10561 gacaaacagc ctcccatgaa tcggcaagta gacacggaac caaatcttcg cgatcgacca
    10621 cgtgcttgag ctcatccacc gatgccgctc tttccatccg tggctgggtg agaatcaggt
    10681 ggtcaacaag aggaagaaaa acgcgcagaa atccttgcag gtttttgtca cgcatcattc
    10741 caacgaccaa aatgagtttt cgtcccgcgc catcatgaat ttgtgagtag agaaagtcaa
    10801 ataacacctg agcaccggca agattgtgag cgccatccac aattaaaagg ggattgcgat
    10861 ccacaacctc taaccgaccc ctccatttga cactctgcaa cccaatccgg atcgcggtct
    10921 gtgaaacagg gaaggtgcca tcaacgccgg tctccaatac cgccagggca ttcgtggcat
    10981 tgaccatctg atgacggccg agcaaattgg cctgtatttc cggaatcgtc catcccagcc
    11041 cttgataggt aaaagttgtc gcactggttt ccaggaagga aaactccttt cccatccgaa
    11101 aaattggcgc tgaccactta cgggcctgag tttcaaatat ctgggcaata ccctccggca
    11161 tcggacccaa cacgatcgga acctttcgct taataacccc ggctttctcc tgcgcaatct
    11221 tctgaagaga gtcgcctaaa tacgcttcat gatcgaagct aattccggta atcaggacac
    11281 cggtggggtt taatacatta gtcgcatcaa agcgtccccc catgccaact tctactacag
    11341 caatgtctac ctgctcattt ctaaaatgca aaaaggccag tgcggtggtg aattcaaaga
    11401 aagtgagtgt ggtgagggaa ttggcagcat gccgaatctg cgcaatcaag ccacagacag
    11461 cctcttcaga aatatctgct ccctgaaccc gaatgcgctc gcggaaatca attaaatgcg
    11521 gtgaggtata tagtccgacc cgatatccgc ttgcttgaag aatcgcagct accatggccg
    11581 atgaggaccc cttcccgttc gtgccggcca catgaaccgt ggcgtaccgg tgctgaggat
    11641 tgtccaaccg gtcaagaata tcggtaatgg actccaatcc cagcttgata ccaaatcggt
    11701 gcaaggaaaa gagaaattcg agcgtttcta gatacgtagc cacaggaatc aaaactaggc
    11761 gggagacaat taagggaagg agatttcgta tcaataataa ctacagtgta tggctctcac
    11821 cattatcggg tctttcgtca agcctgttga gatccacagg ggtacaaggt gaatcggacc
    11881 attcggcggc tacaccgaag aggaagagac tgtggtcaca caatgcgcaa gcaacgtttc
    11941 cagggtcgcc ttgaggattt tccgttccac gatatgatca accatgccat gctccaacaa
    12001 aaactcagca cgttggaagc cttccggcaa atgttctttg atcgtttgtt caataacccg
    12061 tgggccggca aacccgatca gtgccttcgg ttcagccaga atgatatccc ctaacatcgc
    12121 gacactggcg gtgacccctc caaatgtcgg atccgttagc aggacaacaa agggaagtcg
    12181 agcctcttgc aatttcgcca aggctgatgc cgttttcgcc atttgcatga gtgacaaggt
    12241 cccctcctgc atccgtgccc caccggaggt ggtgaccaac aaaaatggca tatggccgat
    12301 caacgcctga tcgattgctc ggcagatttt ctcgccaacc accgatccca tactgccacc
    12361 cataaatgaa aaatcaaaaa ttccaagggc agcgggcttc ccgccgattt gacatcgtcc
    12421 agtcaagatt gcttcttttt ttcccgtttt ttcctgagcc ttttgaatgc gcttacggta
    12481 cggcatcgta tcaataaact gtaaaggatc ccccgggact aactctccat cccattcctc
    12541 gaatgaatcg ggatcgacga tcaactccaa ccgctcaccc accgacaggg gaaaatggta
    12601 ttggcatttc ggacagactt ttaaatttcg ttcaacttcg cgtttaaaaa taatggtgcg
    12661 acagactgcg catttgaccc acatcccttc cacgatttta atggacgtgc cttcggtatc
    12721 cgtgttttta ttccgtttaa accatcccat agatcagtct acccttaagc ttctctcatg
    12781 aacacaatgc acgcgaatcc acatgtagtt ctggcggtga aaaagaaaat tcgagaaagg
    12841 aagggaaaga acacaacaag aaatctgtac ttcgacacag tcttcagtca cgatacgacc
    12901 ttaccatagg gaggttaaac cggcaagaat cccccggaga aggtgcctac accgaagacg
    12961 attccagagt atcagcagag tgcggacagg agaaacaggg agaagacacg agggtcaacc
    13021 accccttgca tgcatttcca gatgtggatc ctggcgcaac cgctcaatgt caatgtaccg
    13081 gccacgaccg ctgacctgtt caagctcttt tcgttcttcc gccccacgat cttttcgaga
    13141 ttcccagaca gactgaatga tggaaagaat atcttctcgt ggtcgttcca tccgaagaag
    13201 cttgcgaagg tcaagacctg ttttggcata caagcataaa taccacatcc catcagccgt
    13261 taaccgactc ctgtcacagg tcgaacagaa aggtactgtg gtggaagaaa taataccaaa
    13321 tgtcgtgccg tcaggcagcc gaaatcgctg tgcaggagcc gagctaacct cctccaccgg
    13381 ttcaaccttc ccataatgat ctccgaccat ctttaaaatc tctttccggg aaagaacctt
    13441 atccgctgac cagtcattgg ctccgccaac gtccatatat tcgataaaac ggacttctcc
    13501 gttgacctgc ttgccgtatt caatcaaggg cagaacttca tcttcgttgt accctttcat
    13561 cgcgaccgta tccaacttta atcctgtaaa tccgactcgc cccaccgagg caattccctc
    13621 aaaaacacga tccaacgtat cgcgtcgagt taacgcctta aaccgctctg gcttcaacgt
    13681 atccagactc actgtgaccc gatggagccc tgcatcgaaa agagcctgag cctgttcact
    13741 caacaacacc ccattggtgg tcatcgcaat atcctggatg cgagagtttt gacgaagcat
    13801 tctaatgagt tccgccacat tattgcgtaa taggggctcc cccccggtaa tacgcactcg
    13861 atcaattccc tgatcggaaa acaaattcgt gagcaaggcg atttcttcaa acgtcaacag
    13921 gttttctcga ggaagccaca cataattttc ctcgggcata cagtactgac accgtaagtt
    13981 gcatcgatcg gtcacggata cccgcaagga tcgcaacgga cgccccaagc tatctctaac
    14041 cgcatccggc agagccacag cttcacccgc ctccattatg gatgttcctc cacccacacc
    14101 tcgccttccg gcgtcgtttc catcttccag atgggagtga tttttttgag ttcatcaata
    14161 caccattggc aggccttgaa ggcatcggcc cgatgttcgg cccccacaat gatcagcaca
    14221 atattctcac caatttcaat tggcccatag cgatggagca ccaaggcctc aattatgtca
    14281 aaatcccgaa gagcctgttc gcgaatttcc ttgagttttt tctgcgccat tccttcgtag
    14341 tgatcaaaac tcatggaact cacgtctcgg cctttggagt ggtcacgggc cgtgcccaaa
    14401 aacatcgcaa tgccaccgat ccgacgggat cgagaccgca cccgatccaa ttcctcatca
    14461 attgaaaaat tttccagttg cacccgaacc aacatgcttt ctacaagatc aacggccatc
    14521 ggattaccct ttctccttag cccatgatac gcaggaccct ccggcaaaag gaggcaataa
    14581 tgctatctcg tcagcctctt taagcactgt gtcccaatgt gcaatttctt gattcaccga
    14641 tatcaatacc ttttttttat cgagtagttc cccaacttga ggatgctcaa cctgaatgga
    14701 gtgaactaga tccctgacct gtccaccttc ggcaatggcg acctccaaat cctttcctcc
    14761 aggaatcatg gtttttaaca ttccgaataa tttaaccttc acaattctac tactcccacg
    14821 accagacaaa gccaacggac atcacccacg gctattttgt cttttgaaca cggacatacg
    14881 taccagactt tccccctgac ttcgacacaa gagcaatttc tccaaactcc atgcccttat
    14941 cgaccgcctt acacatatca taaatggtca gagcggccac ggacactgct gtcatggctt
    15001 ccatttccac tccggtgttt cccgtggttt tcaccgttgc gacaatggta atcgcgtact
    15061 ttccatcaga attaggctga gaactctctt tgaaatctac atccacaccg gtcaacagaa
    15121 ggggatgaca cattgggatc agatcgggag tccgtttggc ccccataacc ccggccactt
    15181 gggctacggc cagcacatca cccttcgcaa ttcgaccctc atgaattttc tcaagggttt
    15241 ccggcaaaat aaacaccttc ccttgggcta ccgctacacg ctcggttgaa cgcttgtcgg
    15301 tgacatccac cattcgagcc cgaccggatt cattaaaatg cgtaagattc gccatattca
    15361 aaaattacca cagataaaaa acctcttgaa aatcagtaga ttataaggac agaggaaaaa
    15421 cgacgtcaag caaaacatac ccttgaattc tcccttgaca cccgacctca ttccacctat
    15481 aaacagtcgg tactaccata ttattcagag atttcaccca tggacaccac cacaattata
    15541 gccaatccta ccctcgcaaa taaaaagcta ggcctcctgc tctcaacctc acctgaacat
    15601 cccaatgcgg aaaccgtcta tcatctcgcc aaaaccgcct tggccaataa ggtagatact
    15661 tatctatatt tcatcgatga gggagttaaa aacctagaag accgaaggtt ttccgagttg
    15721 gcgaaggatg gactgaagtt atttgtatgc gcctatggat gccaacaaca tcacatctcc
    15781 accgacggat atgggaaaga agtgactttt tgcggcttgg tcattctctc aaatatcatc
    15841 gacgggtgtg accgcttttt ggcctttaat taatatctcg cccctcgcct ctcgtccgga
    15901 tcatcatact caatcctgac ttacagacga tattcgcctc aatggagcca gagccaattg
    15961 tgtgctgcta ttttgtggat attttgctgc atggtcttta atccatcgca tgattgtcat
    16021 ctttcccttt tttatgggta taaccaaatt ttccatttga atctaaacca gctagacggt
    16081 acagtacata gccatgctga cttcaacccg ttatttaatt cttgagaccg ccattgacct
    16141 gttgggacgt gacggggcca gcgcgttgac actggagcat gtcgctcggg aagctggact
    16201 gagcaagggt gggttactct atcattttga ggggaaagaa cagttactga cagggctcat
    16261 agagttactc atacaggatt tagaccgcaa acaagttggg aattttctct ccagctccat
    16321 tgatgaagag cttccctggc cagcaattga gactgatata gtcgaaaatc ctataagcca
    16381 tcgctctctg caagggcatg aaattgcctc aattctcatg gctgctcttg cgatcaaccc
    16441 caatctcctt gatcccattc gagagcgata ccaaaattgg caaagcattt ttttggctaa
    16501 taccgttgat ccaacccgct ccctcgttgg acggcttgct accgaagggt tgtggttcag
    16561 agatgcgatg ggattagccc caccaactcg cgagcaacga gctgcgcttg ttctggagat
    16621 ccaatctcaa acacaatcag gagaactcat gagagaacct tccgcaagta tcatacccac
    16681 acagtctaca gacgcctttg cggagctcgc ttcttttaag gcgaatgtag aggaacttga
    16741 acaacaagca tatcaaacca cacaagtttc tgcgcgttca ctattggcga aacaatattc
    16801 tcaagggttt tggcagggcg atcttactgc cgacacaaca ttggaatccg actacgtgtt
    16861 gcttttactc tggctctatc caccggaaaa cggtgtgtgg gagcctcgga ttcagaccaa
    16921 agtgaaggag gccatacgca ccatccttga ccgccaactt tcagatgggg ggtggaatat
    16981 ctactcagag ggcccagccg aggtgaatgc aacaacccgt gcgtacgtgg cacttcgggt
    17041 tggcggccat gatcctgacc aaccacttat gcagcgggca cgtgaatgta ttcttgccct
    17101 tggtgggctt caggcaacca atagctatac caaaaataat ctaagcatgt ttggtctttt
    17161 cccaagaaag tacactccca gtgtgccgcc tgaactcgtc cttatccctg ggaatgtctt
    17221 gtatgaaatt tcatcgtgga gtcgggccat cgtcgtccca ctctccattc ttcaagcgtc
    17281 cggggcacaa cgtagagtgc ctgggaattg gaccgttgac gaactaatgg tgcccaatcg
    17341 gaagctgcga tttccgaaaa aagatccctt ttctctcatg tttcatcaag tggataagat
    17401 tttaaaaatc tgggaaaaac ggggatttaa ggatattcgc gccaaggcga tccgcgaagc
    17461 agagaagtgg atgctggatc acatgcggtt cacggatggg ttgggagcga tttttccggg
    17521 gatgatgtac gccctcatgg cgatggatgc tcttggctat gagcgtgacc atcccgactt
    17581 tattgaaacc cttggccaac tcgaaggact cattcttgaa aaggaaggtg ctctgcagtt
    17641 tcagccaagc ttatcaccgg tatgggatac tgccatctca atgtttgcat tgggagaact
    17701 cggcgttggg gaaccgcagg ctatgcagcg ggcagccgac tggttattaa gcaaagaggt
    17761 tcgccgcaag ggagattggt ccgtaaagcg tccggattta cagcctggag gatggccctt
    17821 ccaatttgcg aacgaactct acccggacat cgacgatacc gccatggtcc tgttggcact
    17881 tgaacatgcc aaggcctcgg atcctgaccg gcagactcgc gccgaaggac gtgccatcaa
    17941 ctggttatta gggatgcagt cctcggacgg aggttgggct gcctttgatg tcgataataa
    18001 ttgggcctta ctgaataagg tcccattcgc agaccacaat gccatgcttg atccaagctg
    18061 cccggacatt acgggacggg tattggaggc actctgcagg cgtgattaca cacaccaaca
    18121 tccggcaatc gcccgtgccg tccaatttct tttgggccac caacaagcca acgggagttg
    18181 ggatggacgc tggggcgtaa attataccta tggaacattc ct
//
LOCUS       DAORAQ010000045        18065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_548_length_18065_cov_66.197446, whole genome shotgun sequence.
ACCESSION   DAORAQ010000045 DAORAQ010000000
VERSION     DAORAQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 18065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18065
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_548_length_18065_cov_66.197446"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="PKM72_06190"
     CDS             complement(<1..1423)
                     /locus_tag="PKM72_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP60410.1"
                     /translation="MHQLLPSTTPSRIRWRILLLLLFISIITYIDRVNISVAARQMMP
                     ALGLTDLQMGQIFSAFVFGYALFQIPGGWLGDRWGPRRVLTFAVIWWSIFTALTAVAP
                     TLPLASLIGIMGSLMVVRFLIGIGEAAALPNFNRAVANWHPPNERGLGIGITIGGIGV
                     GSALTPPITAWIMVNYGWQTAFYVAGGLGIGIALLWYWYATDHPRQHAHVNNAEAELI
                     EGQEFLPSPPAPLEGRSGKAWGMVHDVFTEFETREGRPPASQAKGNGTGGKKNIPTLS
                     TPIRTGRKSEPASIHKSKTTTVPWKAILTTPTVWWLTLSYTCLGYVAYVYMSWFYLYL
                     VNVRGFAILQGAFFASAPFIAMAIFCPLGGWVTDRLTEKYGVNRGRAWVGGAGMILAA
                     LSIIIGANIEAPYVAIGFLSLGAGWLYFTVGPFWSSTTDLSKPYAGTLSGLMNTGANL
                     GGTLSPTLTPWLADTFGWSVSLGI"
     gene            complement(1466..4204)
                     /locus_tag="PKM72_06195"
     CDS             complement(1466..4204)
                     /locus_tag="PKM72_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP60411.1"
                     /translation="MEIITTHLEADFDGLASMVAAKKLYPEAQLVLPAGAQSRERAYL
                     AEHHFSFTALSSLNIPDITRLILVDAQHQDRLGPLEKALKNQHISVHIYDHHPMESTN
                     PLLPRADHCQVDSVGATITLLCEELQTRALTWTPEEATLFAIALYEETGQFVYRNTTP
                     RDFQLGAVLVQSGADLNQVTKYIARPWTPPQLELFHALLQSAQTLDLGHRRILLITLT
                     WPDYVQDMAPIVQQLAQLHGADAIIAAVAMEGKVQIIGRSRHPDMDMNQITQAFGGGG
                     HTMAAAASIKKLTIVEVEQKIRTLLQEQARTWLPIKRLMTTPVRTVDHNTTIKQTERL
                     MTQYEVNALPVVNAKGGFMGLATRESVQKALYHKLTTHPIDHIMLRDIFLATPATPFD
                     EVEQQMIERNQRIVPVLDHDQVVGIFSRTDLLRAMHQALPSQEGDFEPSDRPISLAAT
                     VPSNTLRTSNLHKQLATRLPPPLLALLQTIEKLADTQDVAAFLVGGFVRDFLMNIPNF
                     DLDVVIEGDGIRFGKALAQELKAEWTIHERFGTVSIALPKTLNIPQMHHLDIATARTE
                     YYEYPTALPTVERSSIKKDLYRRDFTINTLAIRLNRTPGELLDFFGGRRDIKDRIVRV
                     LHSLSFVEDPTRVFRAIRFEQRFGFQISKETQHFIQQAQTMELFKRLSGTRLGNELIH
                     ILEEPEPDKGIQRLDQFKLFPFIHPKLQWKEPAPTLFASGEKILAWHEVESARSGTER
                     WLLYALAWFESLGKTELIKTWKRLGFPQRTTVTVGEFLQAQSTLIRTLNRKHLAPSEI
                     YAILTPWPKELVLFLMAKAQSKPTIHAAMERIRDYLTTFQHVAITLTGHDLEDMGLSK
                     GPAYRRVLDRIFKAKLDGLVTTQEDEYRLAKTLIQQETASGSHSKSPSVVRKGGRR"
     gene            4385..5257
                     /locus_tag="PKM72_06200"
     CDS             4385..5257
                     /locus_tag="PKM72_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60412.1"
                     /translation="MAAKAKPKSKAKPKQKPTGKQSAQAKAKPKTKAKPKTMAKSKTK
                     AKPKKPVKPQRSPSIKAKSKNTKVGSKARAKGTAKAGMKGTPKAVAQAQPRSKATPKK
                     VVKGKEKKLTTSDNVSKTTTAAKTGANRTKSAPTKKTAVKPALSEAKKSPGRPKKEST
                     EAKGLNDKKKKITSPVPADEDVSLINADDSDLEDASLPDDIDSELDIEVETDGDITLD
                     SLTTGLDDVSDDLDVDDLADDFDDEFSEDSTDDADEDLDLTEDLSEELGTDKFFRDTE
                     SNHEDDEEEIPRSW"
     gene            5420..6229
                     /locus_tag="PKM72_06205"
     CDS             5420..6229
                     /locus_tag="PKM72_06205"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNP60413.1"
                     /translation="MSEERGLPRIGDHAPDFSAVTTQQTDFNFSVWQEQHWVVLFSHP
                     ADFTPVCTTELIAFSQLNEQFRQRGVKLIGLSVDSIHAHLAWLRNIHDKMGATISFPM
                     IADADMRVARLYGMIHANASSTATVRAVFIIDPKRVIRALLYYPLNAGRNVDEILRLV
                     TALQATDRFACATPANWVEGEKVVVPPPKTVQEADQMFAKTEYEHRDFYLALKDVSPA
                     ATAKPVEVTVPKSAEAAAPEPVKPDPAKPDSIKSEPAKSEPAQPAEPTPST"
     gene            6415..7506
                     /locus_tag="PKM72_06210"
     CDS             6415..7506
                     /locus_tag="PKM72_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNP60414.1"
                     /translation="MKFRGVNYCLLIALLLVLPGLSPINAEACRLLPIPPGSENVFWV
                     HVEGPCLPGEEAALAVKGADLFGALLQGKRIDLDHVLVVDPIMLDLLPQQTLSEYPAI
                     PPVVRQRLQEQGVTAVRVIPEAVSIRHSRFEKVLATNLVEGALLVLGPVDFTGTVFQQ
                     SIDLSKTVFVGPVTFSNARVDFEGFFIGSQFTQGADFSNVTFGTHSRFHQARFWDRVT
                     FADAHFTGVAEFLEVEFQQTADFSRSVFASGTGFSGSVFRGPADFSAIAAAQEIYFRF
                     TEFRESVTFVNAQIHSVVDFTNARFDGAQDFAGVEFRTLPELSGSNLPLNVASVQNVR
                     SLSSQVGIFLGLVILVLCYLWFTKGKKSA"
     gene            7613..8431
                     /locus_tag="PKM72_06215"
     CDS             7613..8431
                     /locus_tag="PKM72_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNP60415.1"
                     /translation="MESVPSLIVSDLEDDDAYQVKLEAFTGPMDLLLHLIRKHQINIY
                     DIPIALITQQYLEYLSMMKTLNLSLAGDFLVMAATLLYIKSRMLLPKEEKPEKEDEEG
                     LDPRAELVRQLVEYERFKEAAGTLVVREQLWRESFSRDPLPLSPEAVIEEDMATEDLQ
                     LFDLLSAFQDVLDRAPTDTIVELSRETWTVQDRIQGILERLEGESTVPFEELFEHHWS
                     KPLVIVTFLALLELVRMNLVRLFQGEWMGPIQVTRRFVPAGGPDSTGSLQAEQR"
     gene            8553..9311
                     /gene="scpB"
                     /locus_tag="PKM72_06220"
     CDS             8553..9311
                     /gene="scpB"
                     /locus_tag="PKM72_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNP60416.1"
                     /translation="MMDALTNGHQASDTLDAEVKEDPTLCEPSEEGCDAEEVTISENV
                     DQKITLDLDELKGAIEALLFVSHDPLSLEKLALVFEGVPKSAVKTAMEYLQAEYEAAG
                     RGLQILEVAGGYVMVTRPEQSVYIKRLTSKAKPAAKVSRSALEALAIVSYKQPITRAD
                     IEKIRGVETSGVLRTLLDQKLIRIVGRQDIPGRPILYGTSKQFLQRFGLRDLRDLPPL
                     KELKELGNPEQFPMELPFAPEENEGESRVTDLSA"
     gene            9415..12222
                     /locus_tag="PKM72_06225"
     CDS             9415..12222
                     /locus_tag="PKM72_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNP60417.1"
                     /translation="MTLLLPEKKPSLGSDLAKRLGQDKVRWDWATLKAYSVDASIYKI
                     PPQVVVLPETEEDIDAVMDYAVKAGVPLTPRAAGTNLTGSAIGAGIIVDISRMNRLLE
                     VNRDEQWARIQPGLVLSEFNKQLAPVGLMYGPDPSSGDMCKLGGMLGNNSAGPHTLRY
                     GSVKDNVHTLRVRLLTEGWLDAQSVDIGGPVHTSLLKGYASLQAVWNIIQDDQELIRA
                     RRPKVSKNSSGYNVFDLVDGLERGIFDVPKLFIGSEGTLGIVSEARVKLVKRPNATVT
                     GLIHFRHLEGMGESVPHLLELSPNALEVMDGNSLNLIGRDRYSIPSDAAATLLIEFDE
                     GEGANRRNRLLEMCRRFPLTGEVRLASDPVQQAELWKARKALFPMLYRFDPQKKPINF
                     VDDVVVPASRTGELIRYLEEYFGRRSVPVAIFGHVGNGNAHIVPLLDLHNQHDVDAMV
                     EAHREIHRTVLDRFDGSICGEHGDGRIRAETVRLMYGKEVYQLFVRVKQAFDSQGMMN
                     PGVKISETSFTEHIDVERMGKSCATCAKCNSVCPVYDVFQSEDMSARGWFEIVTASDY
                     SYLQSERVVEACLNCKSCRTICPAGVDVSELILQRRAEHPNRLAGWIFALHARQWLFH
                     PLLKLMAWTQSFWDRPVIRGMLEKLTRPLLRKLALTAKVPRDMVLPTLAKRHLRDRYP
                     ELCHSEHLRQASVAYFHGCAANYFEDGVGDAVIGLLADYGVTVALPPQRCSGTPIETY
                     GLRDLVKEGARENLAHLASFSTVVTSCASCTLSLKDYATLFHGEPEEAAAIDLRKRVK
                     HISEFLVEQGMASDNTRQTPTPPDGKSAKVTYHSSCHLRAAGVSQAPRDLLASVADLE
                     FVEMQDADRCAGGAGTYMIKNYETSQKIFQRKKRAIQESGAQVVATSCPACMIQLKNG
                     LRGIAEVKHIAQVMRDHSKRK"
     gene            12322..12603
                     /locus_tag="PKM72_06230"
     CDS             12322..12603
                     /locus_tag="PKM72_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNP60418.1"
                     /translation="MVKILIFGLSLREAVEDPEVEISLESPTTIGQLLEDFPDHFQGL
                     MPFLHANELMLTINQKISTLEAMVKNGDTLKITHQGGDQGADGAMWHNP"
     gene            complement(12928..13650)
                     /locus_tag="PKM72_06235"
     CDS             complement(12928..13650)
                     /locus_tag="PKM72_06235"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNP60419.1"
                     /translation="MTIFRVIMSFLLLLLPQIASAQFDDFLKGIDKLSLPGAKTLGDD
                     KIISGLKEALTVGTENTVKLTGNPDGFLKNEAIRILLPEELQSMDKALRLAGFGPQVD
                     ELVVSMNRAAEQAAPLAKPIFKDAVANMSFDDAKKILNGKDTAATDYFQGKTRDQLAI
                     AFKPEVEKTMSQVGVTKQYKDLVGQYTTLPFVTVPAFNLDDYVVGKSLDGLFLILGEE
                     EKKIRTNPTARVTDLLKDVFGK"
     gene            complement(13912..14277)
                     /locus_tag="PKM72_06240"
     CDS             complement(13912..14277)
                     /locus_tag="PKM72_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60420.1"
                     /translation="MGHFGIQKKTPTAGKSPIKHETAEANPSTKVGDPRHSDTCTRCG
                     GFMVSHWCMNVNYDAGGMEILTKRCLQCGEVIDPVILENRLNPQREAMKKKTRPLLSR
                     PLASSTKGKRSGMSCTPIA"
     gene            complement(14623..15180)
                     /locus_tag="PKM72_06245"
     CDS             complement(14623..15180)
                     /locus_tag="PKM72_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNP60421.1"
                     /translation="MRAGITSRLTTDRENGLEAARKQLSARLRLSACLLMILTLVAHS
                     TSTWAATGESSAPLSKFLEIPLEGTSIPDKLRFPLHEQNGVQYFSAGLGKEERSLSYP
                     PYSLKLIFVKGERAFLAGVAVEVINSEQATLVSIPGEEVQGPWLFLNLPPGKYVVKAT
                     DSSGATIEKSITLSGGKTTPVHFRW"
     assembly_gap    15666..15765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15766..16681
                     /locus_tag="PKM72_06250"
     CDS             <15766..16681
                     /locus_tag="PKM72_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNP60422.1"
                     /translation="RRTLPFKWFLQIDGWAVLFYILYGYGVCILNLEFFFEKLAFPFT
                     GISVFGTAVAILLAFRNNSAYERYWEARVIWGDLTNYSRNFASQVLVYIQPPQGSDAQ
                     PEQVEELHREFIYRHLAFLQALRLQLREGYVSNFLSPFITKAELNRLENVANRATQLN
                     HWQAKRLQEVFDAGWIAPRAYVMGLMDSLKYFYIAQGKCERIKNTPLPRQYGFFTKVF
                     VWMFLLLLPFSLVQHLGWEMLPIYVMLAFMFTVMERVGSRTEDPFDGKNEDVPMNAIC
                     RNIEIDLRQQLGETSVPPKIEPIDGVLM"
     gene            complement(16693..>18065)
                     /gene="rmuC"
                     /locus_tag="PKM72_06255"
     CDS             complement(16693..>18065)
                     /gene="rmuC"
                     /locus_tag="PKM72_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNP60423.1"
                     /translation="LVGGTIAWVICSILYRGRKIQALQETATRFASAQAQTGELRTQL
                     ATLQQERDRVHQEFRLMEVAKVSAETNLENTQRHLAEQRALLEDAKLALSDTFRSLAS
                     EALAGNNRGFLTLAEEKFKALKEEATASFDQRHTSIESLLQPLTESLRTYQKESQALE
                     DKRLRELSVVGEQLRQLASSQAILQAETSKLVNALRSPQVRGRWGEIALRRTAELAGM
                     SANCDFFEQESVSTETGRLRPDMIVRLPAGRDVVVDSKVPLNAFLDSLETHTDEDREA
                     ALNRHLGQVKRHINQLASKEYWDQFPAAPEFVVLFIPNDSFLAAAAERDPSLVESALT
                     KKVVIATPTTFIALLRAIAYGWRQEQVAEGAHRISILGQELADRLSTLAEHFGRIGQA
                     LGRTVESYNATVTSLENRIWPTARKFKSLGISAKKDLMDLKSVEHMPRPPGSMDSQPE
                     DFPSSS"
BASE COUNT         4278 a         4117 c         4942 g         4628 t
ORIGIN      
        1 cgatgccgag agagactgac cagccgaagg tatccgccag ccacggcgtc aatgttggcg
       61 agagggtccc acccaggttg gcgccggtgt tcatgaggcc tgagagcgta cctgcgtagg
      121 gtttggataa gtcggtggtt gaagaccaga acgggccgac cgtgaagtag agccaccctg
      181 cgccgagcga aaggaagccg attgcgacat agggcgcttc gatattggcg ccgatgatga
      241 tggagagcgc ggccaaaatc attcctgctc ctcccaccca cgcgcgtcca cgattcacgc
      301 cgtacttttc ggtgagacga tcggtcaccc atcctcccag tgggcagaag atggccatgg
      361 caatgaatgg cgccgaggcg aagaaggcac cctggaggat ggcaaagcct ctgacgttga
      421 cgaggtagag atagaaccag gacatataga cataggcgac gtagccgaga caggtgtaac
      481 tgagcgtgag ccaccagacg gtcggggtgg tgaggatggc tttccagggc acggtggtcg
      541 tctttgattt gtgaatggat gctggttcgc ttttccttcc tgtccgtatt ggggtggaca
      601 aagtggggat gttcttcttc ccgccggttc cgttgccttt tgcctgtgac gctggaggcc
      661 gtccttctcg tgtttcgaat tcagtgaaca catcatggac cattccccat gccttgccgg
      721 atcgtccttc caaaggagca ggaggggagg ggaggaattc ctgtccttcg atcagttcgg
      781 cttcggcgtt gttaacatgg gcatgttgcc ggggatgatc ggttgcgtac cagtaccaca
      841 agagtgcgat gccgataccc agtccgccag cgacataaaa tgccgtttgc cagccgtaat
      901 tgaccatgat ccaggcggtg atgggtgggg tgagagccga acccaccccg atccctccga
      961 tggtgatgcc aatgcctagg ccccgttcgt tcgggggatg ccagttcgcg acggctcgat
     1021 tgaaattggg cagcgccgcg gcttcgccga tgccgatgag gaatcgcacg accatgaggg
     1081 agcccatgat cccgatcaag ctggcgagcg ggagtgtggg cgcaacggcc gtcaaagctg
     1141 tgaagatcga ccaccagatg acggcgaagg tcaatacccg gcgaggtccc catcgatcgc
     1201 ccagccatcc gccgggaatt tgaaaaaggg cgtagccaaa gacaaaggcc gaaaagattt
     1261 gacccatttg cagatcggtg agtccgagtg ccggcatcat ttgtcgggcg gcgacggaaa
     1321 tattcacccg gtcgatgtaa gtgatgatgc tgatgaagag aagcaataga agaatccgcc
     1381 agcggatgcg agatggtgtg gtggacggta aaagctggtg catggggaag gatgggaagg
     1441 cttgccgggg gagccagtga gaaggctatc ttctgccgcc tttccttaca accgaagggg
     1501 attttgaatg acttcctgat gcggtttctt gttggatcaa tgttttagcg agtcgatatt
     1561 catcttcctg tgtggtcacc aggccatcca gtttggcttt gaagatgcga tcgagtaccc
     1621 ggcgatacgc cggacctttg gaaaggccca tgtcttccag gtcgtggccg gttaaggtga
     1681 tggcgacatg ttgaaacgtg gtgagataat cccggatccg ttccatggcg gcatgaatcg
     1741 tcggcttgct ttgcgccttg gccatgagaa ataacacgag ttccttcggc catggcgtga
     1801 ggatagcgta gatttctgaa ggagccagat gctttctgtt gagggtcctt atcaacgtgg
     1861 actgggcctg taagaactct ccgacagtta ccgtggttcg ttgcgggaat cccaatcgtt
     1921 tccaggtttt gatcaattcg gttttgccca acgattcgaa ccaggccagg gcatagagca
     1981 gccatcgctc ggttcctgat cgggcactct ccacctcatg ccaggccagg attttttccc
     2041 cggatgcaaa gagggtcgga gccggttctt tccattgcag tttgggatgg atgaagggga
     2101 agagtttaaa ttgatctaat cgctgaatac ccttgtccgg ttcgggttct tccagaatat
     2161 ggatcagttc attccccagt cgtgtgcccg agagccgctt aaacagttcc atggtttggg
     2221 cctgttgaat gaaatgctga gtttccttgc tgatctgaaa accgaaccgt tgttcgaagc
     2281 gaatggcccg aaagacacgt gtgggatctt ccacgaagct gagactgtga aggacgcgaa
     2341 cgattctatc cttgatgtcc cgccgtcccc cgaaaaagtc cagcagttcg ccgggtgttc
     2401 tatttaagcg gatggccagg gtattgatgg tgaaatcccg ccggtagagg tcttttttaa
     2461 ttgaactgcg ttccacggtc ggtagcgcgg tggggtattc ataatattct gttcgggccg
     2521 tggcaatgtc caggtgatgc atctgtggaa tgttgagcgt cttggggagc gcgatcgaga
     2581 cggtgccaaa tcgctcatga atggtccatt ccgctttgag ttcttgcgcc agggctttcc
     2641 caaaacgaat gccgtcacct tcgatgacca cgtccagatc gaaattggga atgttcatca
     2701 gaaaatcccg gacgaatccg ccaacgagga aggcggcgac atcctgtgtg tcggcgagct
     2761 tttcgatggt ctggaggagg gccagaaggg gtggaggtaa acgggttgcc aattgtttgt
     2821 ggagattgga ggtccgcaac gtattcgagg ggaccgtcgc ggcgagtgaa atcggcctgt
     2881 cagagggctc gaagtcccct tcctgggacg ggagtgcctg gtgcatggct cgaagcaaat
     2941 cggtccggct aaatatgcca acgacctggt cgtgatccaa taccgggacg atccgctgat
     3001 tccgctcgat catttgctgt tcgacctcgt caaacggcgt ggcgggtgta gccagaaaga
     3061 tatctctcaa catgatgtga tcaatgggat gggtggtcag tttatgatac aaggcttttt
     3121 gcacggattc ccttgtggcg agccccatga agccgccctt ggcattcaca accgggaggg
     3181 cattgacttc gtattgcgtc atgaggcgtt ccgtctgttt gattgtggtg ttatggtcca
     3241 ccgtcctgac gggagtcgtc atgagacgct tgatgggaag ccaggttctg gcttgttcct
     3301 gcaaaagggt gcggattttc tgttccacct cgacgatggt gagtttctta atgctggcgg
     3361 cggcagccat ggtgtgtccg ccaccaccaa aggcctgggt gatctgattc atatccatgt
     3421 cgggatggcg gctgcgcccg atgatttgta ccttgccctc catggcgaca gcggcgatga
     3481 tggcatcggc gccatgtaat tgcgccagtt gttgaacaat cggcgccatg tcctgcacat
     3541 agtccggcca ggtgagggtg atgagcagaa tcctccgatg cccgaggtcc agtgtttgcg
     3601 ccgattgcaa caatgcatga aataattcca gttgcggggg cgtccaggga cgcgcgatgt
     3661 acttggtgac ctggtttaaa tcggctcctg attgcacaag gaccgccccg agctggaaat
     3721 cacggggggt ggtattcctg tagacgaatt gaccggtctc ctcatatagg gctatggcga
     3781 ataaggtggc ttcctcaggt gtccatgtga gtgctctggt ttgaagttcc tcgcagagga
     3841 gtgtgatggt ggcccccact gaatccactt ggcagtggtc tgcccgcggg agtaggggat
     3901 tggtggactc catggggtga tggtcataaa tatgaactga aatatgttga tttttaagag
     3961 ctttttcaag cggtcccaat cggtcctgat gctgggcatc aactaatatc aggcgggtga
     4021 tatccggaat gttcagactc gaaagtgccg tgaaggaaaa gtgatgctcg gcgagatagg
     4081 cccgctcacg ggattgggct cccgccggga ggacgagttg tgcctccggg tagagctttt
     4141 tggcggccac cattgaggcg aggccatcaa aatccgcctc gagatgcgtg gtaataattt
     4201 ccatggaagt cggattctaa cagggatccc gaatgaccgg aagtttcttg aaaaggcgaa
     4261 gaatttcctc attgcaaata ttcagttgct tgagagggtc cctccatgtt agatgggggc
     4321 aactcaggag gcgatccatc ccagatggat cattggtcga tcacataaat caggagggtg
     4381 acacatggct gccaaggcta agcccaagtc aaaggccaag cccaaacaga agcctacggg
     4441 aaagcaatcg gctcaagcaa aagctaagcc gaagacgaaa gccaaaccga agacgatggc
     4501 caaatcgaag acgaaagcca aaccaaagaa gcctgtgaaa cctcaacgat caccgtctat
     4561 caaagcgaag tccaagaata cgaaggtcgg ctccaaggct cgggccaagg gaactgccaa
     4621 ggctggaatg aagggtactc ccaaggccgt ggctcaagct caaccacgat ccaaagcgac
     4681 gccgaaaaag gtggtgaaag ggaaagagaa aaagttgact acatctgaca atgtttcaaa
     4741 aacgacaacg gcggccaaaa caggggcgaa tcgtaccaag tctgctccca ccaagaaaac
     4801 ggcggtgaag ccggcgctat ccgaggccaa aaagagccca ggaagaccta aaaaagaatc
     4861 aactgaggca aaaggtctta acgacaaaaa gaaaaaaatt acctcacctg ttcctgccga
     4921 tgaggacgtg agtttgatca atgctgatga ttcggatttg gaagatgcat ctctgccgga
     4981 tgacattgat tccgaattgg atattgaagt ggaaaccgat ggtgatatta ccctggattc
     5041 attgactacc ggactcgatg atgttagcga tgatctggac gtggatgatc ttgcggatga
     5101 ttttgatgat gagttctccg aggactccac agatgatgcc gatgaagatt tagatctgac
     5161 ggaggactta tcagaagaat tgggcaccga taagtttttt cgggacaccg aatctaatca
     5221 tgaggatgat gaagaggaaa ttcctcgctc ctggtagctc tgctggccta taatgggtcc
     5281 aggtttggtc aaggtctctt tctcagccat ctcgatcgtg ttgatcgtat tgccggtttc
     5341 ataataaata aaccgggatt tctccattcg cctgaagcgg agccggagcg ttattgtatt
     5401 gccaactgaa ggaggattca tgtcggaaga acgagggttg cctcgcatag gagatcatgc
     5461 accggacttt tccgcggtga cgacgcaaca gactgatttt aattttagcg tatggcagga
     5521 acagcattgg gtggtactgt tttctcatcc ggcggatttt actccggtgt gtacgacgga
     5581 attgatagcc tttagccaac tcaacgaaca atttcgtcag cggggggtga agctcattgg
     5641 actgagtgtg gatagcattc atgcccattt agcctggctg cgaaatattc acgataaaat
     5701 gggtgccact atttcgtttc ccatgattgc cgatgctgat atgcgcgtcg ccagactgta
     5761 tggaatgatt catgccaacg cgagctcgac cgcgacggtg cgtgcggtgt ttattattga
     5821 cccgaaacga gtgatccggg cgttgctgta ttacccactc aatgcgggac gtaatgtgga
     5881 tgaaattctt cggttggtca ccgccctgca agcgactgac cggtttgcct gtgcgacgcc
     5941 agccaattgg gtggaaggag aaaaggtggt ggtgcctccg cccaagacgg tgcaagaagc
     6001 cgatcagatg ttcgcgaaaa ccgaatacga acatcgggac ttttatttag cgctcaagga
     6061 cgtttctccg gctgcgacgg caaagccggt ggaagtgacg gtgcctaaat ctgcggaagc
     6121 cgcggctccc gagccagtta agcccgatcc ggctaaaccc gattcaatta agtccgagcc
     6181 agctaaatcc gagccggctc aacctgctga gcccacccct tctacttagg ggctatttga
     6241 gatgagattg aggtctacga ttgtgccgga ttcagccaaa ttctccgatc atgctgttca
     6301 agaggcccac tcgcccaacc cgatcgaaaa tttgactcaa cccgacgctg cctcgtgagg
     6361 atcatctatc ttcaacgcgt tccttgggta tgtgctcatg tgatgtgggc atagatgaaa
     6421 tttcggggcg tgaattattg tctgctgatt gccttgctgc tcgtcctgcc ggggctgtcc
     6481 cctatcaatg ccgaggcttg ccgtcttctt ccaattcctc cgggaagcga aaatgttttc
     6541 tgggtgcatg ttgagggacc ttgtttgcct ggagaagagg cagccttggc cgtaaagggg
     6601 gctgatcttt ttggtgctct cctgcaggga aaacgcattg atcttgacca cgtgttggtg
     6661 gttgatccga taatgcttga tctcctgccg caacaaaccc tttcggaata tcccgccatt
     6721 ccccctgtag tgaggcagcg cctgcaagag cagggtgtca ctgcggttcg cgtcattcca
     6781 gaggctgttt ccatcaggca ttctcgtttc gaaaaagtgc ttgccaccaa tctggtcgaa
     6841 ggtgctctcc tggtgctggg accggtagat ttcacgggaa ccgtctttca gcaatccatt
     6901 gatctttcta agactgtgtt tgtgggtccg gtaacatttt caaacgcgcg tgtggatttt
     6961 gaagggtttt ttattggatc gcaatttacc caaggcgcgg atttctctaa cgtcactttt
     7021 gggacacatt cccgttttca ccaggctcga ttttgggatc gtgtcacctt tgccgatgct
     7081 catttcaccg gagttgcgga gtttttggaa gtcgagttcc agcagacggc agatttctcc
     7141 cgatcggttt ttgccagtgg gacaggcttt tcagggtcgg tatttagagg acctgccgat
     7201 ttttcagcca tcgcggctgc tcaggaaatc tattttcggt tcaccgaatt tcgagagtcg
     7261 gtgacatttg ttaatgctca aattcactca gtggtcgatt ttacgaacgc caggtttgac
     7321 ggagcgcagg attttgcagg ggtagagttc cgtaccctgc cggaattaag cgggagtaat
     7381 ctcccgctta atgtggcgtc ggttcaaaat gtcaggagcc tatctagtca ggtgggaata
     7441 tttttgggat tggtcatttt ggtgctctgt tacctctggt ttacaaaagg gaagaagtcc
     7501 gcttgaaata aggctcaacc actatatttg gttaggatat tgcggcttta cacaaaagat
     7561 ggtataccaa ttcaattatc tgcactgtgg aacccagtcg aaaggctcct gattggaatc
     7621 cgtcccctcc ctgatagttt ctgaccttga agatgacgat gcctatcagg tcaaacttga
     7681 ggcctttacc ggccccatgg atttgctcct ccacctgatt cggaaacacc agattaatat
     7741 ttatgatatt ccgattgcgc tgattactca gcaatacttg gaatatttgt ctatgatgaa
     7801 gacgttaaac ctatcgcttg ccggggattt tctggttatg gccgccacgc ttctctacat
     7861 taaatccagg atgcttcttc ccaaagagga aaaaccggag aaggaggatg aggaaggtct
     7921 cgatcctcgc gccgaattgg tcaggcaatt ggtcgaatat gagcgtttta aggaagcggc
     7981 cgggactctg gttgtccgag aacaactttg gcgagagtcc tttagtcgtg atccgctccc
     8041 cttgtctccg gaagccgtga tcgaagagga tatggctaca gaagatctcc agttatttga
     8101 tcttctgagt gcgttccagg atgtactgga tcgagctcct accgatacaa tcgtcgaatt
     8161 gtccagggaa acctggacgg ttcaggaccg tattcaaggc attttggagc ggttggaagg
     8221 ggagtcaaca gttccctttg aagaattgtt tgaacaccat tggtccaagc cattggtcat
     8281 tgtcacattt ctggctttgt tagagctggt caggatgaac ctggttcgtt tgtttcaagg
     8341 agaatggatg ggtcccatcc aggtgacgag gcgttttgtg ccagcgggag gacctgattc
     8401 cactggaagt cttcaggcgg agcagaggtg atacgcatga gccggatatt aaattggagg
     8461 atgcatgggg ggacttagga atagcctggg tgaatgttgc ctgtggcagt gtgggatgaa
     8521 tcggttcagt ccgcagggga ggactatata acatgatgga cgcactcacc aatggacacc
     8581 aagcttcaga tactctcgat gccgaggtga aggaagaccc caccctatgt gagccctcag
     8641 aggagggttg tgacgcagaa gaggtcacga tttccgagaa cgtagaccag aagatcacgt
     8701 tagaccttga tgaactcaaa ggtgcgattg aggccctgct ttttgtttcg catgatcctc
     8761 tgagtcttga aaagctggct ctggtcttcg aaggagtccc taaatcggct gttaaaaccg
     8821 cgatggaata tctccaagcg gagtatgagg cggctgggcg tggcttgcag atattggaag
     8881 tggccggagg gtatgtcatg gtgacgcgac ctgagcagag tgtgtatatc aagcgattaa
     8941 ccagcaaggc caaaccggca gccaaggttt cccggtcagc attggaagcc ctggccattg
     9001 tgagttataa acagcccatt accagagcag atattgaaaa aattcgaggt gtagaaacct
     9061 ctggcgtatt acggactctc ctggatcaga agttaatccg gatcgtcgga cggcaggata
     9121 ttcccggtcg accgattttg tatggaacga gcaagcagtt tcttcaacgg tttggcctgc
     9181 gggacttgcg agatttgcct ccactgaaag aactcaaaga gttgggtaac cctgagcaat
     9241 tccctatgga gcttcccttt gcacctgagg aaaatgaggg agaatcccgg gtcaccgatc
     9301 tttccgccta aatcgacagt gaggccacgc gttctgctgg tctgcttgtt ttcctccctg
     9361 ttttctctct gacaaggttg tgtccgatcc gcgtaggaag cagaaagagt tcctatgacc
     9421 ctgttactac ctgaaaagaa accttccctg ggctccgatt tggccaaaag attggggcag
     9481 gataaagttc ggtgggattg ggccacgctg aaagcctatt ccgtcgatgc cagtatctac
     9541 aaaattcccc cgcaagtggt tgtgcttcct gaaacagaag aggacattga tgccgtgatg
     9601 gactatgccg tcaaggccgg agtgcctctg actccacggg cagccggaac gaatctgacc
     9661 ggatcggcca tcggggccgg tattatcgtg gatatctcca gaatgaatcg tttacttgag
     9721 gtgaatcgag acgagcagtg ggcccgaata caaccaggcc tggtgctatc cgagttcaat
     9781 aagcagttgg cacctgtggg tttgatgtat gggccggatc cttcaagcgg ggatatgtgc
     9841 aagttaggag gcatgctcgg caacaattcc gctggtccac atacgcttcg ttatggatca
     9901 gtcaaggaca atgttcatac tctccgggtt cggctgctca ctgagggttg gcttgatgct
     9961 cagtctgttg atattggagg accggttcat acctccttgt tgaaagggta tgcctcctta
    10021 caggcggtgt ggaacatcat tcaagatgac caagagttga tccgcgccag acgtcccaag
    10081 gtgagtaaga atagttcagg ttacaacgtc tttgatctgg tcgatgggct ggagcgaggc
    10141 atatttgatg ttcccaaatt atttatcggt agtgagggca cgcttggcat tgtcagtgaa
    10201 gcgagagtca agttggtcaa acgacctaat gccaccgtga cgggactgat ccattttcga
    10261 cacctggagg gtatgggcga atcggttccc cacttattgg agttgagccc caatgcgttg
    10321 gaggtgatgg atgggaattc gttgaattta atcgggcgag atcgctatag cattccctca
    10381 gatgcggcgg ccacgctgct catagaattt gatgaaggcg aaggcgccaa tcgtcgtaac
    10441 cggctgctgg aaatgtgccg aaggtttcca ctcaccgggg aagtgaggtt ggcctccgac
    10501 ccggtccagc aagcggaatt gtggaaggcc cgcaaggcat tgtttccaat gctctatcga
    10561 tttgatcctc aaaaaaaacc catcaatttt gtcgatgatg tcgtggtgcc cgcctcacgc
    10621 accggagagt tgatccggta tttggaagag tattttggac gacgatcagt accggtcgcc
    10681 atttttggac atgtgggaaa tggtaatgct catattgtgc cgttgctcga tttgcataat
    10741 caacatgatg ttgacgcgat ggtggaggcc caccgcgaga ttcaccggac ggttttagac
    10801 cggtttgacg gatctatttg tggagagcat ggagacgggc gtattcgggc cgagaccgtt
    10861 cgcttgatgt atggcaaaga ggtatatcag ctttttgttc gagtgaagca ggcgttcgat
    10921 tcgcagggga tgatgaatcc tggtgtgaaa atcagtgaaa cgtcgtttac ggaacatatt
    10981 gatgtggagc gtatgggcaa atcctgtgca acgtgcgcca aatgtaattc tgtgtgtccc
    11041 gtgtatgatg tgtttcagtc tgaagatatg agtgcgcgcg gatggttcga gattgtgacg
    11101 gcttcggatt actcctacct gcaatccgaa cgggtggtcg aagcctgttt gaattgcaag
    11161 tcctgccgga ccatttgtcc ggccggggtc gatgtgtccg aactgattct tcagcgacgt
    11221 gcggaacatc ccaaccggct ggccggttgg atctttgccc tccatgcccg tcaatggctt
    11281 tttcatccct tattgaagct catggcatgg acccaatctt tttgggatcg tccggttatt
    11341 cgaggcatgt tggaaaagct gactcgcccg ttgttgcgaa agttggctct cacggcaaag
    11401 gttccccgag atatggttct gccgaccctt gctaaacggc atttgcgtga tcggtatcct
    11461 gaactgtgtc attccgaaca tctccgacag gcttcagttg cgtattttca tgggtgtgcg
    11521 gccaactatt ttgaggatgg agtgggggat gcggtgatcg ggcttttagc cgattatggt
    11581 gtgacggtcg ctcttcctcc acaacgatgc tctggtaccc cgattgaaac ctacgggttg
    11641 agggatttgg tcaaagaagg cgcgcgagaa aatctggcac acctggcgtc attttccacc
    11701 gtggtcacca gctgtgcctc ctgtacgtta agcctgaaag attatgccac cctgtttcat
    11761 ggggagccgg aggaagccgc agctattgat ttacgtaaac gagtgaagca tatctctgaa
    11821 tttcttgttg aacaggggat ggcgtcagac aatacgcgcc aaactccgac gccgcctgat
    11881 ggaaagtcgg cgaaagtgac ctaccattcg tcctgccatc ttcgcgccgc cggtgtgtcc
    11941 caagcccctc gagatctgct agcctcagtg gccgaccttg aatttgtcga aatgcaggac
    12001 gccgatcgat gcgcgggagg agcgggtacg tatatgatca agaattatga aacctctcag
    12061 aaaattttcc agcgtaagaa gcgggctatt caagagagcg gggctcaggt ggtggcaacg
    12121 agttgccctg cctgtatgat tcagttgaaa aatggacttc gtgggatagc agaggtcaaa
    12181 catattgcgc aggtcatgcg ggaccattca aaaaggaaat aaaggtgatg ggacattggg
    12241 tctcaggtgt gtgcttcggc aggataacgt tgttatagtt gagattggga tcgaaatgta
    12301 tttttggacg agggacttcg aatggtaaaa attttgatat ttggtctttc acttcgtgaa
    12361 gcggttgaag accctgaggt ggagatttcg ttagagagcc caaccactat cggccaattg
    12421 ctagaggact ttccagatca ctttcaaggg ttaatgcctt tcctccatgc caatgaactc
    12481 atgttgacaa ttaatcagaa aatttccacc ttggaagcca tggtaaaaaa tggggatacc
    12541 ctgaaaatta ctcatcaggg aggtgaccaa ggggcggacg gagcgatgtg gcacaaccct
    12601 taattctgca cgggttgcga tgaatatttt gtttggtggg gagaaaagtt atggattagg
    12661 aaatgttggt tagattgacc cttttgaggg gaggggtgat tcctcgtcac attttttgca
    12721 attgagaatc gttttgagat gtgctcgccg gccttctggc gataagaccc agggccgaaa
    12781 actgagtggg ggttgatgtc ggacatgctg cccgtggcca caggccagat cggctaccca
    12841 atcatctaca tcatcctgat ggaatccgat aatcggttgg ttcattttcc ctaagacctc
    12901 tataatttgg gatgataaat ttgcaaatta ttttccgaac acatccttga gtaaatctgt
    12961 gacgcgtgct gtggggtttg ttcgaatttt tttctcttcc tcccccagaa tgagaaacag
    13021 cccgtccaag cttttcccga caacatagtc gtccaaatta aacgcgggta ctgtgacaaa
    13081 cggtagggtc gtatactgtc caaccaaatc cttatattgt ttggttaccc cgacttgact
    13141 catggtcttc tccacttcag gcttaaaggc tatggccagt tgatcgcggg tttttccttg
    13201 gaagtaatcg gttgctgcgg tatcttttcc attcagaatt ttcttggcat cgtcaaagct
    13261 catgttggcg accgcgtctt taaatatagg cttagccaag ggagccgcct gttcagcggc
    13321 tcggttcatg ctgaccacca actcatcaac ctgagggccg aatccagcta gtcgaagagc
    13381 tttgtccatg gattgaagtt cctccggtag taagattcta atggcttcat tttttagaaa
    13441 gccgtctggg ttgccagtga gtttcacggt attttccgtc ccgacggtga gagcttcttt
    13501 aagaccggat atgattttgt catcaccgag ggtttttgcg ccaggaagag ataatttatc
    13561 gatccctttc aaaaaatcat caaattgtgc cgaggcgatt tgtggaagga gtaaaaggag
    13621 aaaactcatg atgacgcgaa aaatggtcat tgttctctcc ttttatatgc agaaacggta
    13681 agagggtcgt atgaggaaaa agggaataag agggggaaag actgtcagaa acgctcatct
    13741 catcttcctc tgagatggga cccacaagga agaggggagc gttgcgccct gggattatcc
    13801 tggggaggat agactaaaca acctaagcag tcttcaaatc ctcaggaggc aaattccggc
    13861 tcttcctcgt gggtggttca aaaagcaccc tcgagtatga gatgcctaaa gttaagcgat
    13921 aggagtacaa ctcatccctg atctcttgcc tttagtcgat gacgctaaag gcctggagag
    13981 gagcggacga gtctttttct tcatcgcctc tcgttgaggg ttgaggcgat tttccaatat
    14041 tacgggatca atgacttcgc cgcattgcaa gcatcgcttg gtcaaaattt ccattccacc
    14101 agcatcatag ttcacattca tgcaccaatg ggagaccata aaccctccgc accgtgtgca
    14161 tgtatccgaa tgccttggat cccccacttt tgtggaagga ttggcttcag ccgtttcatg
    14221 cttgatggga cttttccctg ctgtcggggt ctttttttgt attccaaaat gtcccatatg
    14281 tatctccttt ttcctgattg tcatgacata tctaatataa tgtcttgttg ttagatatag
    14341 ctctttgagc ttaagtcaag attaatccaa tattaaaaaa tcctcataat tgtatttaca
    14401 gaacaacaat aactttcata agttggaata aacctttaag aattaaacag attgaatcga
    14461 taattagtat atacagctac gtccttaatg ctaagtaaaa aaacccataa aataaaagaa
    14521 cgaaaaaatg aggagaccaa ggggaactgg tctctcaaga gcctgagttt gggggtaaat
    14581 tacggaaaat cccgaaaatt cctggagatg aaaacggtcc aactaccagc gaaagtgaac
    14641 aggtgtggtc tttccccctg atagggtaat gcttttttca atagtggccc cacttgaatc
    14701 ggttgctttg accacatatt ttcccggagg aaggttgaga aataaccaag gaccttgtac
    14761 ctcctcaccc ggaatagaca cgagcgtggc ctgctccgag ttgatcactt caactgccac
    14821 gccagctaaa aaagcacgtt cacctttcac aaagatcaat ttaagggaat agggtgggta
    14881 tgacaggctc cgttcctctt tacctaatcc ggcgctgaaa tattgaacac cattctgctc
    14941 atgcagaggg aatctgagtt tatcagggat acttgtacct tccagtggaa tttctaaaaa
    15001 tttggagaga ggtgccgagc tttcacctgt ggcggcccag gttgacgttg aatgagccac
    15061 caaagtcagg atcatcaaaa gacacgcact tagtctcaat ctggcagaca actgtttcct
    15121 tgctgcttcc aggccatttt ccctatctgt tgtcaatctg ctagtgattc ctgctctcac
    15181 gtgcgatacc tcatcctttc caataaagaa aaatctttaa ccaaaacttg gatttactaa
    15241 ggatataata ccaatttcag gttacttaag aaagaatagc gcaaacagtc aaaaggccaa
    15301 gttctaggct cattagcaat tgaaagaagc gaggtggcat ggaattctct agcgcgtcag
    15361 gcaaaaagga aggccgagga atgcatctaa ggcgatattt cctcgagcgt cagcacgcta
    15421 cccaccgaat gaatctaaat atatggcatg tcaacaaaac agtgattctt ggttttcaat
    15481 gatcagtcgt tgatagcaga gtccttcggg aaaatcgttg ggcctgagca atgcggcgag
    15541 taaattcctc ttaacttaac cattccccat tctgtgtttg aggtcagccg gagacctaac
    15601 atgtgaccgg ggtgagtgtg ttatcccagt tgtgcttgat ggcataagac tcaaacttaa
    15661 aggatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagac gaacgcttcc
    15781 gttcaagtgg tttttgcaaa ttgacggttg ggctgtcttg ttttatatcc tgtacggcta
    15841 cggagtctgc atactaaatt tagaattctt ttttgaaaaa ctcgcatttc cctttaccgg
    15901 aattagtgtg tttggaacgg ctgtcgcaat tttattggcg tttcgaaata attcggccta
    15961 tgaacggtac tgggaggcca gggtcatatg gggtgatttg actaattatt caagaaattt
    16021 tgcctcacaa gttcttgtct atattcaacc gccccaaggc agcgatgctc agcctgagca
    16081 ggtcgaggaa ctacatcgag agtttattta tcggcacctc gcatttttgc aggctttgcg
    16141 tttacaactg cgggagggtt acgtgtcgaa tttcctttct ccattcatca cgaaggccga
    16201 gttaaatcgg ttggagaatg ttgccaatag agccacgcag ctgaatcatt ggcaagcgaa
    16261 gcgattacag gaagtgtttg acgctggctg gattgcgccg cgtgcctatg tgatgggact
    16321 gatggattca cttaagtatt tttacattgc ccaagggaaa tgtgaacgaa taaaaaatac
    16381 acccttgcct cggcagtatg ggtttttcac caaggttttt gtgtggatgt ttctgttact
    16441 tcttcccttc tcattggtgc aacatttggg atgggaaatg cttcctattt acgtgatgct
    16501 ggcattcatg tttacggtta tggaacgagt gggaagtcga actgaagatc cctttgatgg
    16561 aaaaaacgag gatgttccca tgaatgctat ttgccgaaat attgaaattg atttacgaca
    16621 acaactggga gaaacgtctg tgccacctaa aattgaacct attgatggtg tattaatgta
    16681 aaggatgcct gtttatgagg aagaaggaaa gtcttccggc tgggaatcca tgctaccagg
    16741 tggcctgggc atgtgttcta cggattttag gtccatgagg tctttttttg cgctgattcc
    16801 gagtgatttg aattttctgg ccgtgggcca tatccgattt tccaatgaag tgacagtcgc
    16861 attataggac tccactgtcc gacccaaggc ctgtccaatt ctcccaaaat gctctgccag
    16921 tgtgctcagg cgatcagcga gttcttgtcc gagaatgctg atgcgatggg caccttcagc
    16981 tacttgttcc tgacgccatc cataggcgat ggctcgcaat aaggcaatga atgtggtggg
    17041 tgtggcgatg acgacttttt tcgttaaggc cgactcgacg agtgaagggt cccgctctgc
    17101 ggcggccgct aagaacgaat cattggggat aaataagacc acaaattccg gagcggctgg
    17161 aaattgatcc caatattcct ttgaggctaa ttgattgata tgccgtttca cctgaccaag
    17221 atgccgattg agcgcggctt cccggtcttc gtctgtatgt gtttccaggc tatctaaaaa
    17281 cgcattgagc ggtactttgg aatcgaccac cacatctctt cctgccggga gcctgacgat
    17341 catatctgga cgcaaccgtc ctgtttcggt ggagacgctt tcctgttcaa aaaaatcgca
    17401 gttggctgac atgccggcta attcagcggt tcttcgaagt gcgatttccc cccagcgtcc
    17461 tcgaacctgt ggtgaccgta aggcattgac caatttagat gtttccgcct ggaggatggc
    17521 ctgggaagag gctaattggc gtaactgttc tcccacgaca ctgagttcgc gcagccgctt
    17581 atcttctaat gcttgagatt ctttttggta ggtacgtagg gattcggtga gcggctgcaa
    17641 taaggattca atggaggtgt ggcgttggtc gaaggaagcc gtcgcttctt ctttgagagc
    17701 tttaaatttt tcctcggcca gggtcagaaa tcccctattg tttcctgcca gggcttccga
    17761 ggctaacgat cgaaaggtat ccgataaggc caattttgca tcttccagta aggcccgctg
    17821 ttcggctaag tggcgttgcg tattttcaag gtttgtttcg gctgacactt tggccacttc
    17881 catcagacgg aattcttgat ggacccgatc acgttcctgc tgcagggtgg ctaattgggt
    17941 ccgaagttct ccggtttggg cctgcgccga ggcgaatcga gtggcggtct cttgtaaggc
    18001 ttggatcttc cttcctcgat agagaatgga acagatgacc catgcgattg tccctccaac
    18061 cagca
//
LOCUS       DAORAQ010000046        17483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_568_length_17483_cov_68.595306, whole genome shotgun sequence.
ACCESSION   DAORAQ010000046 DAORAQ010000000
VERSION     DAORAQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_568_length_17483_cov_68.595306"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1030)
                     /gene="trpC"
                     /locus_tag="PKM72_06260"
     CDS             complement(185..1030)
                     /gene="trpC"
                     /locus_tag="PKM72_06260"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNP60424.1"
                     /translation="MILSRILEHKKAELRRKQSRGYLAELKGRIVDRTRPLGFIQALE
                     TGSTSAAPALIAEVKKASPSQGLMRPEFHDRFEPVIIASQYRDHGASALSVLTDRDFF
                     QGDLEYLHNVKEAVQLPTLNKEFMIEEIQFYEARAYGADCVLLIAAALDRFQLEDFFT
                     FAKELSLDVLIEIHDERELDTVLERVPLARLIGINNRDLKTFQTDLGVTERLAKRMPK
                     DTLIVSESGIHSRAHVVRILEAGAKAMLIGESLLRADSIQSKIQELLHPPTSPKKEVS
                     TTRWV"
     gene            complement(1027..2052)
                     /gene="trpD"
                     /locus_tag="PKM72_06265"
     CDS             complement(1027..2052)
                     /gene="trpD"
                     /locus_tag="PKM72_06265"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNP60425.1"
                     /translation="MPILKNHIAKLVEGLDLSELEAEEAMREIMQGEATEAQIAAYLM
                     GLRMKGETIDEITGSVRAMRERAIRIPITDPQVVDTCGTGGDKSHTFNISTASAFVVA
                     GGGMTVAKHGNRSVSSQSGSADVLAALGVNIDLSPEKVADCINEIGIGFLFAPLYHGA
                     MKYCAKPRTELGIRTLMNIMGPLANPARASIQILGVYDHTLNEKLAQVLLRLGTQHCF
                     VLHGMDGLDEISLTSRTGISEGKKGRVLSYSIGPEDFGLHPVSPKELLGGSPEDNAQI
                     IRDIFRGRKGPKRDIVLMNAAPAFIACQKATSLKEGFEEAGRVIDNGAAFEKLDKLIS
                     LTKKLAA"
     gene            complement(2087..2674)
                     /locus_tag="PKM72_06270"
     CDS             complement(2087..2674)
                     /locus_tag="PKM72_06270"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNP60426.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELGADLQIFRNDALTIEDIHRLAP
                     ERILISPGPCTPNEAGISVATIKHFAGRLPLFGVCLGHQSLAYAFGGQIIRAPRLMHG
                     KTSMVHHDGKTIFESLPNPFEATRYHSLIVKRETLPTEFEISAETVEGEIMGLRHIPT
                     GAEGVQFHPESILTTAGMDLLRNFLLMPVCSSTDH"
     gene            complement(2695..4191)
                     /gene="trpE"
                     /locus_tag="PKM72_06275"
     CDS             complement(2695..4191)
                     /gene="trpE"
                     /locus_tag="PKM72_06275"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNP60427.1"
                     /translation="MKNAYYSVSFDEFCQFAAQGNLVPLYREILADFETPVSAFSKIN
                     TGANAFLFESIEGGENWARYSFLGSNPSVMLWEENGEVVTQQGRKQHRVPLQGNPLDH
                     IQNIMAEYHPVVVPGLPRFVGGAVGYLGYDVVQSFEPIPSRPKNGIKTPLFAFCITDT
                     LLIFDNIAHTLKIVANAHITSTKKTHLRQIFRDAINRIEFIIARLHKPTRRPTPPTRR
                     GPLKFQSNMSPEDFEKMVIRTKEYIQAGDIIQGVMSQRWQTTIHTNPLEIYRALRVLN
                     PSPYMFYLRIAGIELVGSSPEILVRCEEGNIVVRPIAGTRPRGQTPQADAALEQDLLS
                     DHKELAEHVMLVDLGRNDVGRVAKAGTVKLERFMKVERYSHVMHIVSQVKGELDPQYT
                     AYDVMKACFPAGTVSGAPKIRAMQIIEELEPTRRGPYAGAVGYFSFSGNMDTCINIRT
                     VVVQGQKAYIQAGAGIVADSDPTREYEETRTKAGAMMKAIGMAEHGLA"
     gene            complement(4345..5001)
                     /locus_tag="PKM72_06280"
     CDS             complement(4345..5001)
                     /locus_tag="PKM72_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNP60428.1"
                     /translation="MTQPLFGGCQEDPQPSGWCGYLKIVAGVLVCSGVLSGCVSTEKF
                     EAEKARALNFQRLLAQEEKRTGELNVKYQDTQRQMSSMESQNRDLNAELEALRDQLNR
                     SHDELSRVREGGMEKSDDLKLSEPSISEFGLNDLDFNDSDMTKLDSDLNLGDNVPLDS
                     PMMDTLGGPSDGSHIVAKGETLYRISKEYGVSVDDLKSWNQLTDNTIHVGQKLIVNGQ
                     "
     gene            5244..6200
                     /locus_tag="PKM72_06285"
     CDS             5244..6200
                     /locus_tag="PKM72_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP60429.1"
                     /translation="MIPPFLHSSDPSVMHIGQVALRLARPLALQQAWMGDQNILRQLL
                     ACWFIVDEKDVPLSPRIVGQPGVGKTTLAMAATQERKQELFIYQCTADTRPEDLLVSP
                     VISEGGTITYQASPLVTAMLTGNVCLLDEGNRMNEKSWASLASLLDHRRSVDSVVAGV
                     QIHAHENFRCCVTMNEDASTYEVPDYILSRLQPTLKVEFPNKEHELAILRYHLPFASA
                     ELITLTVNFLQKAHQLDLPFSIRDGMHMVQYAMKRMAQDPHHPVARDPAWQEALINVL
                     GEEARDLDVLAKRRSQTLHGQALPKGLGDFFFEEDNPLHPDQ"
     gene            6291..8186
                     /locus_tag="PKM72_06290"
     CDS             6291..8186
                     /locus_tag="PKM72_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60430.1"
                     /translation="MTLAALTPDRIFALSSHIHVLPVVHGSGDMAHIVREIIVSRHID
                     CVALPLPPSVQPLVEEGINQLPIISLVVLPEQNDAGTSSCSYVPIDPCQPVIMGIRSA
                     MSEGIPRAYVDREVQHYHSVSWVGPDPYTLKTVPLAAFSAATMPFLPPPQDQSSRWER
                     IRWMAFRLHELELDFSAVLFLCPMTDWPWLRQAYHERMPYIPPEQRMGLPEWWKVEPA
                     SLYFGLSELPYVTQLYEQRREEARADTHLAIVGMKELVLEARARWMASRSTTIAQETN
                     WVTPQLLQRYFQYVRNLTLLEHRLKPDLYTLVLAAKQMAGDEFALRLLETAKTYDYQT
                     QPSVLDTRPAVLLGIGELQDPDGNILPAVNRLQGDPLVWRRVTLRPDPPRPKTQSWAQ
                     QWNPYRQCSWPPEDQRIESFAAHVRQHSRQILGADLGRIERFTNSLEDGIDLRATLRQ
                     WAISPSRSVRDVHVKVVPPVRGTIEALVFFFEVPADPQTFSWQTTWYAEHQEESTLSF
                     YATPFTTNLVGPGIGEACYGGALFLYPPRLIPDIWENPAFDFATSLEERLLAGACAYS
                     QEPFVAVVSPVPLTSVWRKIANRFGRKLLPIPLHRFSGQTIARLRRFHVLNGHDIRSY
                     AAKFIRE"
     gene            8161..10287
                     /gene="ligA"
                     /locus_tag="PKM72_06295"
     CDS             8161..10287
                     /gene="ligA"
                     /locus_tag="PKM72_06295"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNP60431.1"
                     /translation="MQPSLFENNSGETEEESSTKTPHSPAVPSIKVKQRLEELKHAIR
                     RHDELYYVQSRPEISDAEYDQLFRELQDLESHYPDLRTPDSPTERVGGTPLVQFSKIP
                     HEFPLLSLDSEMDEESVLAFDQRVCRELEVSQLSYTAEPKYDGLSVALTYDNGMFVRG
                     ATRGNGAIGEDVTHNLRTIRALPLQLKECGTYPSRVVVRGEVFMKLPDFHQLNRRLTE
                     QGEEPFANPRNASSGTLRQLDPAITATRPLTITCYDFMSPGPGRPTTHFEAVTCLEAW
                     GLPIPQFRRHCPSIQEAIKFHREMFERRDVLPFEIDGIVIKIDRYDWQKTLGEKSRSP
                     RWAIAFKFPPRKELTKVQEIAMSVGRTGALTPIALLDPVEIGGVTVSRASLHNVEEVA
                     RKDVRVGDTVKVERAGDVIPDVVERVPVPGEERGAPFQPPDICPVCQSHTIQEGPILY
                     CTGQTVCSAQLKGSLEHFGSKSALNIEGLGKKTVAQLVDKGLVKKLSDLYTLDVNDIL
                     LLEGFAAKSAKQLLGEIEKSKNVPFGRLLIGLGIRHVGTHIARILAKEFVSLDNLRRA
                     TEGELLLIRDIGPEIAASVTHFFQEPRNLIVWQHMESLGVRVQQEANVVEPHAQPLRG
                     KLFVLTGTLKGYSRQEAKRRIEELGGRVTSSVSKQTDYLVAGEDPGSKYDSATKLGVR
                     IIDENEFFLFSQSGNLEPSPSNEKNF"
     gene            complement(10427..11704)
                     /locus_tag="PKM72_06300"
     CDS             complement(10427..11704)
                     /locus_tag="PKM72_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNP60432.1"
                     /translation="MDFIAVLICLGLSAFFSGAEIAFFSITESRLKALADEGHKGAKL
                     ALKLRSNPQRLLSTILIGNNLVNILAASLATLIAIRMFGSEAVAVATGILTFMVLLFG
                     EIVPKTLCAKYSETAVQLLAYPIYLLEQLFFPFLYFLEPFILKLTGGRGLTVPFITEE
                     ELKIMLDAGGKAGVLETDEVRMIKNVFDFNDVTAEDAMTPRIYMFALDGNQTLGEARE
                     ELFKAKYSRIPIYEGNHDNITAILYRNHALMELAQSHTNLTLKSLAKPALFIPSTKTA
                     DDLLRQFQQEKRHIAIVVNEFGGVMGLITVEDLLEEVVGEILDEGDLHEEWIRRTGKN
                     QILVDGRTEVRRINEFLKVELDEEEQNTISGLILEELGHIPAIGEKVETDGCLLVVHD
                     ADERSIKSVQIIKEEPIPETPPTEPSTVSSESS"
     gene            complement(11951..12625)
                     /locus_tag="PKM72_06305"
     CDS             complement(11951..12625)
                     /locus_tag="PKM72_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNP60433.1"
                     /translation="MNSGIPVKRICLILTFMLMTGCAGGGHSRTEPMISETSQQGVAS
                     WYGPSFHGNPTANGEPYNMWALTAAHRTLPFGTRVLVQSVETGKSVTVRINDRGPFIR
                     GRIIDLSYGAARELAMIGKGTENVRLTILESPSAGSGQLSNGESVMYWVQVASYTTLT
                     QAVSMREQLATHYPNIRLTTVDLPSGQWHRVQVGTFPSQEKAKFVAKELERQFDLDPL
                     LIQVTY"
     gene            complement(12729..13964)
                     /locus_tag="PKM72_06310"
     CDS             complement(12729..13964)
                     /locus_tag="PKM72_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60434.1"
                     /translation="MTQEDARAYLNYLLTLHLRQEEAFGPLGLAFVRENDLTELALIP
                     EEQFNLLMAIATVFSAEPKRYTMKLELLQKAYQLLPNTRYNDPELGRDLEHLIRKTQS
                     DLHRYNEAMKVARAQSSDRQSLVVETDIPEYFLETAQKRASAYYQEKYRLTKEAKTAQ
                     HFGGTAKKFEPDNPAIHKEFPGACAPFINARTNAFHIVLPFDLKISRSPEDPLEAGIR
                     IFYGKMGYSFPLRYEMGKLCSYHDGQVLDVDLHDPNLIFFSVSGIKDPEFIIQASQTD
                     PSLPPELVYPMTVLEHTGSLGPFIQVSCNIKVWFDASMVSLLLQGAPDLPDYGLQGGA
                     GLMTRTYASDKVESYVHNLSQPWQEGLSFNFVNLHLQLSPGIDSAVVPFGTPIFSVYP
                     VFNRQSYRFVDRRTMDQAP"
     gene            14134..14550
                     /locus_tag="PKM72_06315"
     CDS             14134..14550
                     /locus_tag="PKM72_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60435.1"
                     /translation="MQQDFLQKPRGAVLILGWIIACLFLGIGVTFVSAEDSAVKKPGP
                     VAAGGEDFSTENLNKQITDLETQIQKLRDQSIELQEKTRAKLQAQLDNFKKQQDTLIP
                     RIEQLRDNSETAWQDIKENIQKAIEDLKLSVDSMKK"
     gene            14730..17141
                     /locus_tag="PKM72_06320"
     CDS             14730..17141
                     /locus_tag="PKM72_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNP60436.1"
                     /translation="MVQHTEQVLEWPVLIDCLAKEASSSLGEGCCRTLAFASDLHSAV
                     IQQQETSEMVHILQGPHPLPSLVFPDIRNVLARAQKEGILEGPELRDISLVLGLGHTI
                     KEYLQIHGSTFPKIRTRSLNLQDLIWVKQTIDSCIDLNGQLRESASPELYQLVRKSQG
                     LRQTIRRRLETILASQEYEDLLQGQYFAERENRYVIPIKAERQQAVNGIVHDISSSRA
                     TVFVEPRHLVELNNSIKFVDLQVAQEERHLLQDLSSRVAEHVSAIHGNLSCLADLDCL
                     MAKARLSIKTHGSPILLNQEHCIDLQQARHPLLALTKEQVIPNSIRIEDETKVLIISG
                     PNAGGKTVSLKLVGLFAMMVKVGLHPTCGPDSKMALFEQVYADIGDSQDLRNDVSSFS
                     GHILNMIALLKNVESSGEPTNRDALVLLDEVGSSTDPIEGAALAEALLTRLCELGCTI
                     VATTHYPSLKTLPFRNPHVRNASQEFDMVTLAPTYRLIDGIPGGSSALDIAERLGLDS
                     TIIQSAKSLIRGQDFDLDAIFRTLQNTHTRLERECEQAQYLRQEAQRLLEEAQVTRNQ
                     LKAQEQEDRQRYRKQWQQEFSKAQRHLNQIVNDLKKDKTLLKVKSIQQTIGTLNQEIL
                     SHLPNNQWVSSEPLHEGDLVEINALGTIGVLQESLEGKRQVSIRVGTRTIKTAPSAIR
                     RAIPSSDNKPLSGQQQLSGKRALPTISTELASPASNQETGLYEQELVVRGFRFDDAME
                     MTLAALDHALVTQAKYLKVIHGQGSGALKTGIRTLCQSSPYIQSFRAGDPSEGGDGVT
                     VIELR"
     gene            complement(17133..>17483)
                     /locus_tag="PKM72_06325"
     CDS             complement(17133..>17483)
                     /locus_tag="PKM72_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60437.1"
                     /translation="YLLDAERCISYLTIEYRGSVDDLPAELRKKIGNRIFGCDDCLDI
                     CPFNVNANPTTEAGFQPFPWTLHPQLPSLANLTKDEFHSLTKGSPLRRPKHEGFIRNV
                     QIGLKNHAPRIPLS"
BASE COUNT         4473 a         4193 c         4233 g         4584 t
ORIGIN      
        1 atgccacaaa tttttatttt aggcgacata ggaacgttcg aggttggcca ggaaaattct
       61 caattatcat ggtgtaccgc ttcaatttaa ttcctttgcc ttggttatat aggctccact
      121 tcatgttcgg ccagtctctg gaaaaagaga acgagtacat aagggaccgc ccttcaatcg
      181 aatcctaaac ccaacgagtg gtggatactt cttttttggg agatgtcggg ggatgtaata
      241 attcctgaat tttggattgt atcgagtctg cgcgcaataa cgactcaccg atcaacatgg
      301 ctttggctcc ggcttccagt attcgcacga catgagcccg gctgtggatt ccactttcac
      361 tgacaataag tgtgtccttc ggcattcgtt tggcaagccg ctcggtgaca cccaaatcag
      421 tctggaaggt tttcaaatcc cggttgttga tgccaataag tctggctaag ggcaccctct
      481 caaggacggt atctaattcc cgttcatcat ggatttcaat gagcacatca agggataact
      541 cttttgcgaa agtgaaaaag tcttcaagct ggaagcgatc caacgccgcc gcaatcagta
      601 acacacagtc ggctccatag gcccgcgctt cataaaactg aatctcctcg atcataaatt
      661 ctttattgag cgtgggaagt tgaaccgcct ctttcacatt gtgcaaatac tccaagtccc
      721 cttgaaagaa atcacgatcg gtgaggacgg ataaggcact tgctccatga tcacgatatt
      781 gagaggcgat gatcactggc tcaaaccgat cgtgaaattc cggccgcatc agtccttgac
      841 tgggtgaagc ttttttgact tcggcaatta atgcgggagc tgctgaagtc gaccctgtct
      901 ccaatgcctg aatgaaaccc agtggtctgg ttcgatctac aatgcggccc ttgagttcag
      961 ctaggtagcc ccggctctgt tttcgacgca attcggcttt tttatgttcg aggatacgag
     1021 aaagaatcat gccgccagct ttttggtcag ggaaatgagt ttgtccaact tttcaaatgc
     1081 ggctccgttg tctatcacac gaccggcctc ttcgaaccct tcttttagcg atgtcgcctt
     1141 ctggcaggcg ataaatgccg gcgcggcatt catgagaacg atatcccgtt tggggccttt
     1201 tcgtcctcga aaaatatcgc gaatgatctg ggcattatct tccgggcttc ctcccaatag
     1261 ttctttgggc gaaaccgggt gaaggccaaa atcttccggc ccgatggaat agctcaggac
     1321 gcgtcctttt tttccttcgg aaatccctgt gcggctggtt aaagaaattt catctaaccc
     1381 gtccattcca tgaaggacaa agcaatgttg tgtgcccaaa cgcaacagta cttgagccaa
     1441 tttttcgttg agagtgtgat catacacacc cagaatctgt attgaggccc gggccggatt
     1501 agcaagcggt cccatgatat tcatcagcgt gcggatcccc aactctgtcc ggggtttggc
     1561 gcaatatttc atggctccgt gatagagggg tgcgaagaga aagccaatcc caatttcatt
     1621 gatgcaatcg gcaacttttt caggagacaa atcgatattc acgcccaggg cggccaacac
     1681 atctgcgctc cctgattgag aggacaccga gcgattccca tgcttggcaa cggtcatccc
     1741 tcctccagct acaacaaatg cggaagccgt agagatattg aacgtatggc ttttgtcccc
     1801 accggttcca cacgtatcca ccacctgagg atcggtaata gggattcgaa tcgcccgttc
     1861 gcgcatggct cgaaccgatc cggtgatttc atcaatggtt tcgcctttca tgcgcaaccc
     1921 cattaagtac gcggcaattt gagcttcagt ggcctctcct tgcatgatct cccgcatagc
     1981 ttcctcggct tcaagctctg agaggtcgag gccctcgact aatttggcaa tatggttttt
     2041 gagtatgggc atgtgaacag gcttatgaac agatgacttg ggggctttaa tggtcggttg
     2101 aagagcagac gggcattaat aaaaaattcc gcaatagatc catccctgcc gtggtgagaa
     2161 tcgattcagg atgaaattgc acgccttccg ctccggttgg gatatgccgg agtcccatga
     2221 tttcgccttc cacggtttcg gctgatattt caaattccgt gggcagggtt tcacgtttga
     2281 cgattaagga atgatatcgt gtggcttcaa agggattcgg gaggctttca aaaattgttt
     2341 ttccatcatg atggaccatg gaagttttcc cgtgcatgag tcggggagcc cgaataattt
     2401 gacctccaaa ggcgtaagcc agagattgat ggccgagaca gacaccaaat aagggaagac
     2461 ggccggcaaa atgcttgatc gtcgcaacgg aaatgcccgc ctcgttgggg gtgcaggggc
     2521 ctggtgaaat taatattcgc tccggtgcca ggcggtgaat gtcctcgatt gtgagggcat
     2581 catttcgaaa aatctggagg tcggctccca actctccaag atattggaca agattgtagg
     2641 taaatgaatc gtaattatcg atgaccagga tcataaattt cttctctagc ttttttacgc
     2701 taggccgtgt tccgccattc caatggcttt catcatggct ccggcttttg ttcgagtttc
     2761 ttcatattca cgggtagggt cggaatcggc gacaattccg gcgccggcct gaatataggc
     2821 cttctgtcct tgaacgacga ccgtacgaat attgatgcag gtgtccatat ttccggaaaa
     2881 gctgaaatac ccgaccgctc ccgcataggg ccctcgacga gtgggttcca actcttcaat
     2941 gatttgcatg gctcggattt tcggtgcacc agatacggta ccggctggaa aacatgcctt
     3001 catgacatca taggcagtgt attgcggatc taattctccc ttgacttgag aaacaatgtg
     3061 catgacatgg gaataccgtt caactttcat gaaccgctca agcttgaccg ttccggcttt
     3121 ggccacccgg cccacatcat tccggcctaa atccaccaac atgacatgtt cggctaattc
     3181 tttgtgatcg gatagaagat cctgttccaa ggccgcgtca gcttgcggcg tctggccacg
     3241 gggacgggtc ccggcgatgg gccgcaccac aatattgcct tcttcgcatc gtacgaggat
     3301 ttccggagaa gatcccacga gctcgattcc tgcaattcgt aaatagaaca tgtagggcga
     3361 gggattgagc acccgtaagg ctcgatagat ctccagggga ttcgtatgga tggtggtctg
     3421 ccatcgttgg gacatgacgc cctgaatgat gtcgccagcc tgaatatatt ctttggtccg
     3481 aatcaccatt ttttcaaagt cctcagggct catattcgat tgaaatttga gtggcccacg
     3541 tcgagtcggg ggcgttgggc gcctggtcgg tttatgaagc ctggcaatga taaattcaat
     3601 ccgattgatg gcatcccgaa agatctggcg aaggtgggtc ttcttcgtgg aagtaatatg
     3661 agcgttggcc acgattttga gggtatgggc aatattgtca aaaatgagta aggtgtccgt
     3721 aatgcaaaat gcaaagaggg gagtcttgat cccatttttc ggtcgagatg gaatcggttc
     3781 aaacgattga accacgtcat aacccaaata gccgaccgca ccgcccacaa atcgtggcaa
     3841 accgggaacg acaaccgggt ggtactctgc cataatattt tgaatatggt ccaatggatt
     3901 cccctgcagc ggaacccgat gctgtttccg accttgttgg gtgacgactt cgccattctc
     3961 ctcccacaac atgacggaag gattactacc caggaaggaa tatcttgccc agttttctcc
     4021 cccttcgata ctttcaaata agaaagcatt ggctcccgta ttgattttgg aaaatgccga
     4081 cacaggcgtc tcgaaatctg ccagaatttc tcgataaagt gggacaagat ttccttgtgc
     4141 ggcgaattgg cagaattcgt caaaactcac tgaataataa gcatttttca tggttttgac
     4201 ggtaccatac ggcctataag gtgtcaacga aagcggtgga gagacacgcg gttttcagag
     4261 ggagggataa gaaaaggccg gaaccggaag aagaaagctt cctgaagatt ccgacctaat
     4321 cttaataaat gcgtagaggt gaaattactg accgttgacg atgagctttt gtccaacgtg
     4381 aatggtgtta tcggtcaact gattccagga tttgagatca tcaacggata cgccatattc
     4441 tttggaaatc cggtagaggg tttctccctt agccacaata tgtgatccat cgctaggacc
     4501 tccaagagta tccatcatag gcgaatccaa aggaacgttg tctcccaagt ttaagtccga
     4561 atcaagtttc gtcatatctg agtcgttaaa gtccagatca ttcagcccaa attcagagat
     4621 cgaaggttcg gaaagtttca aatcatccga tttttccatc cctccctctc gcacacgcga
     4681 aagttcgtca tgggaacgat tgagttgatc tcgtaatgct tctaattcgg catttaagtc
     4741 ccgattttgc gattccattg agctcatttg tcgttgcgtg tcctgatact tcacatttaa
     4801 ttcaccggtc cgtttttcct cttgcgccaa aagtcgttga aagtttaacg cacgagcctt
     4861 ctcggcctcg aacttttctg tactgacaca tccagacagg accccgctac agactaatac
     4921 cccagccaca attttcagat atccacacca gcccgatggt tggggatctt cttgacatcc
     4981 cccgaacaat ggttgcgtca tgttacgcat tcctcctctg aggttcatat ttatcccttc
     5041 tatagggaca atggtcaata taatataacg agaacgcgaa gatctccacg gtctagtcac
     5101 cttttgctac acaataattc cgaataagga taaagtcaaa caactatttt tcacaccagc
     5161 agaagttccg aaacaccacg attcattgac catagaaaaa tggctgtgat aatttcactt
     5221 acagaaattt ttcgtccttt tctatgatcc ctccattttt acattcttcc gatccttcgg
     5281 tgatgcacat cggacaggtt gctcttcgcc tggctcgtcc tcttgccctt caacaagcct
     5341 ggatggggga tcaaaatatt ctacgtcagc ttctcgcctg ttggtttatt gttgatgaaa
     5401 aagatgtccc cttatcacca agaattgtcg gtcaaccggg tgttggaaag accacactcg
     5461 ccatggcggc gactcaggag cgaaagcagg aactcttcat ttatcaatgt accgctgata
     5521 ccagacccga agatctcctt gtctcccccg tcatttctga aggaggcacg attacctatc
     5581 aagcctcgcc cttggtcacg gccatgctga ccggcaatgt ttgtcttctt gatgaaggca
     5641 accggatgaa tgaaaagagt tgggcctccc ttgcctcact gcttgatcat cgacgatcag
     5701 tcgattcggt ggtggcgggt gttcaaattc atgcgcatga gaatttccgg tgttgtgtca
     5761 ccatgaatga agatgcctca acctatgagg tgccggatta tatcctgtcc cgcctgcaac
     5821 ccaccctgaa agtagaattt cctaacaagg aacatgagtt agccattttg cgatatcacc
     5881 tcccgtttgc gtctgcggaa ttgatcacgc tcacggtgaa ttttcttcaa aaagcgcatc
     5941 aactggatct cccgttctcc attcgggacg ggatgcacat ggtgcaatac gccatgaaac
     6001 ggatggccca agaccctcac caccctgtgg cccgtgatcc ggcctggcag gaagccctga
     6061 tcaatgtgtt gggagaagaa gcccgggatc tggatgtgtt agcgaaacgc cgatcccaga
     6121 cattgcacgg gcaagctctc ccaaaaggat taggggattt cttctttgag gaggacaatc
     6181 ctctgcaccc ggaccaatag tagctcatta accctgggcg acaccgatga acggcccatt
     6241 ctctaattct tgagtggaaa gggctccccc gttttcctcc agaacgcact atgactctcg
     6301 ccgcgttgac cccggacagg atctttgcct tatcttccca tattcacgtc ttgcctgtgg
     6361 tgcatggaag tggagatatg gctcatattg tgcgtgagat tattgtgtcg cgccatattg
     6421 attgtgtggc ccttcctctt ccgccatctg tgcaacccct cgttgaagag gggattaacc
     6481 aattgccaat catcagtctg gtggtccttc ctgaacaaaa tgacgctgga acatcgagct
     6541 gttcctatgt gcccattgat ccctgccaac cggtcatcat ggggattcga tctgccatgt
     6601 ctgaagggat cccgcgagcc tatgttgatc gggaggtcca acactaccat tccgtctcct
     6661 gggtcgggcc tgatccctat accctaaaga ctgtcccttt ggcagccttc tcggctgcca
     6721 ccatgccttt tttgccgcct cctcaagatc agtcctctcg ttgggaacgc atccgttgga
     6781 tggcatttcg actgcatgaa ctcgaactgg acttttctgc tgtacttttt ctttgtccaa
     6841 tgacagattg gccctggttg cgtcaggcct atcacgagcg gatgccgtat attccacccg
     6901 aacagagaat gggccttcct gagtggtgga aggtcgagcc ggcttcgctc tactttgggt
     6961 tgagtgaatt accctatgtc acgcaattgt atgaacagcg acgggaggag gcccgagccg
     7021 acacgcattt ggctattgtt ggcatgaaag aactggtttt ggaagcacga gcccggtgga
     7081 tggcgtctcg ttctacgacc atcgcgcaag agactaattg ggtcaccccg caattactac
     7141 aacgctattt tcaatacgtg cggaatctga cgcttctcga gcatcgtctt aaaccggatt
     7201 tgtatacctt ggttctagcc gcaaaacaga tggctggtga tgaatttgcc ctcagattat
     7261 tggaaaccgc caaaacctat gactaccaaa ctcaaccatc cgtactggat actcggcctg
     7321 ctgtgttgct gggaattggc gaacttcagg atccggatgg aaacattctg ccggctgtga
     7381 atcgtttgca aggagatccc ttagtttggc gccgtgtcac cctcaggccc gatcctccaa
     7441 ggcccaaaac acaatcttgg gcacaacaat ggaatcctta ccggcagtgt tcctggcctc
     7501 cggaagatca gcgcattgaa tcgtttgcgg cgcatgttcg tcagcatagt cgacagatct
     7561 tgggtgcgga tttgggaaga attgaacgat ttactaactc actggaagat ggaattgacc
     7621 tgcgggcgac tttgcgccaa tgggccattt ccccgagccg gtcggtgcgc gatgttcatg
     7681 ttaaagtggt tcctcctgtg cgcggaacta ttgaagcgtt ggtgtttttc tttgaggtgc
     7741 cggctgatcc tcagacgttt tcctggcaaa cgacctggta tgccgaacat caagaagaat
     7801 ccaccctcag tttttacgcg acccccttta caacaaatct ggtcggaccg ggaatcgggg
     7861 aggcatgcta tggcggagcg cttttcctgt atccaccacg actcattcct gatatatggg
     7921 aaaatcccgc atttgatttt gcgacgtcgc ttgaggaacg gttgcttgcg ggagcctgcg
     7981 cctattcaca ggaacccttt gtggctgtcg tctctccggt tccgctgact tcagtctgga
     8041 gaaaaattgc caatcgtttc ggccggaagc tcctccccat tcctttgcac cggttttcag
     8101 gtcaaacgat tgctcgtcta cgacgatttc atgtgttaaa tggacacgac atccgaagtt
     8161 atgcagccaa gtttattcga gaataactct ggcgagacgg aagaagagag ttctaccaag
     8221 actcctcact ctcctgctgt gccatccata aaggttaaac aaaggttgga agagttaaaa
     8281 cacgccatcc gtcggcatga tgaattgtat tatgttcaaa gccgacccga aatctctgat
     8341 gctgaatatg accagctctt tcgggagctt caggacttag agagccatta cccggacctc
     8401 aggacacctg attcgccaac cgagcgggtc ggaggaacac cgcttgtcca attcagtaaa
     8461 attccccacg aatttcctct tctcagtttg gattcggaaa tggatgaaga gagtgtattg
     8521 gcttttgatc agagagtttg ccgggaactg gaagtgagcc agctcagcta tacggctgag
     8581 cccaaatacg atgggttatc tgttgcactc acttatgaca atggcatgtt tgttcgaggg
     8641 gccacgaggg gaaatggtgc aataggggag gacgtgacgc acaatcttcg taccatacgt
     8701 gccttaccat tacagttaaa agaatgcggg acctatccct cccgtgtggt ggtgcggggg
     8761 gaggtgttta tgaagctccc cgattttcac caattaaatc gacgcttaac tgagcagggg
     8821 gaagaaccct ttgccaatcc gcgcaatgcg tcatccggaa cattacgcca attggacccg
     8881 gccatcaccg cgactcgccc gctgacaatc acctgctacg atttcatgag tcccgggcca
     8941 ggaaggccta ccactcattt tgaagctgtc acatgcctgg aggcatgggg attacccatt
     9001 cctcaatttc gccggcactg tccctccatt caagaagcta taaagtttca tcgtgagatg
     9061 tttgaacgac gggatgtttt gcctttcgaa attgacggca ttgtcatcaa aattgaccgg
     9121 tacgattggc aaaagactct tggcgaaaaa tcacgcagtc cccgatgggc catcgccttc
     9181 aagtttcctc caagaaaaga actgaccaag gttcaggaga ttgccatgtc ggtcggtcgg
     9241 accggggcac tcacccccat tgccttactg gacccggtgg aaatcggtgg tgtgacggtg
     9301 agtcgagcct ccctgcataa cgtggaagaa gtggctcgta aagatgtgcg ggtgggtgat
     9361 accgtcaagg tcgagcgagc aggggacgtc attccggatg tggtggaacg agtgcctgta
     9421 ccgggtgaag agcgtggggc tccgtttcaa ccgccagata tatgtccggt ttgtcaatca
     9481 cataccattc aggaagggcc aattctgtat tgtacgggcc aaacggtgtg ttcggcgcaa
     9541 ctcaagggat cacttgaaca ttttgggtca aagagtgctt taaatatcga agggcttgga
     9601 aaaaaaacgg tagcacagtt ggtcgacaag ggcctcgtaa agaagttgtc cgacctgtat
     9661 acattagacg tcaacgatat ccttctattg gaaggttttg cagccaagtc cgccaaacaa
     9721 cttttaggag aaattgaaaa gagtaagaac gttcccttcg ggcgccttct gattggactc
     9781 ggaatacgtc atgtgggcac tcacatcgcc agaattctgg ccaaggaatt tgtttcgctc
     9841 gacaatttga ggagagctac cgaaggggag cttttgctaa ttcgtgacat tggaccggag
     9901 attgctgcga gtgtgactca tttttttcaa gaaccacgta atttgattgt ttggcaacac
     9961 atggaatcat tgggagttcg ggttcaacag gaagccaatg tggtagagcc tcatgcccaa
    10021 ccgctaaggg gaaagctttt tgtgctaacc ggaactttaa aaggttattc ccgtcaggag
    10081 gcgaaacgca gaattgaaga gttaggtgga cgtgtcacat ccagcgtcag taaacagaca
    10141 gattaccttg tcgccggcga agaccccggc tctaaatatg acagcgccac aaaattaggc
    10201 gtgcgaatta ttgacgaaaa tgaattcttt ttattcagtc aatcagggaa ccttgaaccc
    10261 tcaccctcca atgagaaaaa tttctaacga ataatctaag agggtttttt ttcaccagga
    10321 aagaatgagt gacccaaaac ggatggacca atctctcggc tggaaataaa aaggggagac
    10381 cacttttact tttagctgca ggaaaaagga agaaaccgag aaggtgttat gaggattctg
    10441 aagacacggt ggacggttcg gtgggagggg tttccggaat aggctcttcc ttgatgatct
    10501 gcacgctttt gattgagcgt tcatctgcgt catggaccac gaggaggcaa ccgtcagttt
    10561 caactttttc gccaatggcg ggaatatgtc ctaactcttc caggatgagc ccgctaatgg
    10621 tattttgctc ttcttcatct aattcgactt taagaaattc attgatacgt cggacttcag
    10681 tccggccatc cactaaaatt tgatttttcc ccgtccggcg aatccattct tcgtgaagat
    10741 cgccttcatc cagaatttct cctaccactt cttcaaggag atcttcaacg gtaattaaac
    10801 ccatcactcc accaaattca ttaaccacaa tcgcaatatg ccgtttttct tgttggaatt
    10861 gccgcaataa atcatcagcc gttttggtag agggaatgaa caatgcaggt ttcgccaatg
    10921 atttgagggt aaggtttgta tgactttgag ccagttccat cagggcatga ttgcgataga
    10981 gaatggcggt gatattatcg tgattccctt cgtaaatggg gatccgtgaa tatttagctt
    11041 taaataactc ttcccgtgct tctcccagtg tttgattgcc atcgagggcg aacatataaa
    11101 ttctgggagt cattgcatct tcagccgtca cgtcattgaa atcaaatacg tttttaatca
    11161 ttctcacttc atccgtctct aagactcccg ctttacctcc ggcatcgagc atgatcttga
    11221 gttcttcttc cgtaataaac ggcacggtta acccacgacc cccggtgagt tttaaaataa
    11281 acggttccaa gaaatagaga aaaggaaaga ataattgttc taggagatag atggggtatg
    11341 ccagtaattg gacggccgtt tctgaatatt ttgcacagag cgttttgggt acaatctccc
    11401 cgaacaagag caccataaaa gttaatatcc ccgttgccac agcgacagcc tcagaaccaa
    11461 acatccgaat ggcaatcagc gtggccagag atgccgccaa aatgttgacc aaattattgc
    11521 caattaaaat agttgagagt aggcgctgtg gatttgagcg taatttcagg gcaagttttg
    11581 cccctttatg accttcatcg gccaaggctt tcaaccggct ttccgttatt gaaaaaaagg
    11641 cgatctcggc gccggagaaa aaggctgaaa gtcctagaca aataaggacg gcgatgaaat
    11701 ccataggggg attaacgagc tccgacgtga caaagagatg gcgttacagc acaaatagct
    11761 gcaagatctt gtagtctggt ctttgaggaa gttggtatca gaagtgtttg agagggattc
    11821 ataataaggc caaaagtctc atggtttatg agatattttg agccctcact atttgcttgg
    11881 gtccaacgta ccatagggga ttggtttaag gcaattttac tggatgaatt atcagattaa
    11941 ctcttttgtt tcagtaggtt acctggatca gaagtgggtc gagatcaaat tgtctttcca
    12001 gttctttcgc cacgaatttt gccttttcct gagagggaaa tgtcccaacc tgaacacggt
    12061 gccattgtcc ggatggaagg tccacggtcg tcaatcgtat attgggatag tgggtggcca
    12121 gttgttcccg catggagact gcttgggtta aagtcgtgta agaggctacc tgcacccaat
    12181 acataacaga ctctccattc gacaattggc ccgaacctgc gcttggagat tcgagaatcg
    12241 tcaacctgac gttctccgtt ccttttccaa tcatggccaa ctcccgagca gctccatagg
    12301 acagatcaat aatccgacca cgaataaaag gaccacgatc attgatgcga acggtcacgg
    12361 atttcccggt ttcgacactt tgaacgagga cccgtgttcc aaagggaagc gtacgatgcg
    12421 cggcagtgag tgcccacatg ttatagggtt caccattggc agtcggatta ccatgaaaac
    12481 ttgggccata ccaggaagcc actccctgtt gagaagtttc tgagatcatc ggctcagtac
    12541 gggaatggcc acctcctgcg catccggtca tgagcatgaa agtcaggata agacaaatcc
    12601 ttttgactgg aataccacta ttcatgaccc gtattccccg ccggagtatg aagggaaggt
    12661 gtttttttcg agttggataa ccttgggaac cgcatgagcc catggactca agcaatagcc
    12721 atggatttct agggagcctg gtccatcgtt cggcggtcga caaagcggta gctctgtcga
    12781 ttgaacaccg gatatacgga aaatatcggg gttccaaagg gtacaaccgc actgtcaatg
    12841 cctgggctta gttgaagatg aagattcaca aaattaaaac tcaagccttc ttgccaaggt
    12901 tgagataaat tgtgcacata tgactcaact ttatcggaag cgtacgtcct ggtcatgagg
    12961 cccgctcccc cttgtaatcc ataatcaggt aaatctggtg caccttgaag gaggagcgaa
    13021 accatagagg catcaaacca caccttgatg ttacaactga cctggataaa tggccccaaa
    13081 ctcccggtgt gttctagcac ggtcatggga tagaccaatt ctggtgggag agaagggtcg
    13141 gtttgagagg cttgaatgat aaactctggg tccttgatac ccgatacaga gaaaaagata
    13201 agattcggat cgtggaggtc cacatccagt acttggccgt catggtaact gcataatttg
    13261 cccatctcat agcgtaaagg aaatgaatat cccatttttc catagaaaat tcgaatccct
    13321 gcttccaacg gatcttccgg gcttcgacta atttttaaat caaatggtaa cacaatatga
    13381 aaagcattgg tccgggcatt aataaatgga gcgcaggcac cgggaaattc cttatggatc
    13441 gcaggattgt ccggttcaaa ctttttcgcc gttccaccaa aatgctgggc agtttttgcc
    13501 tctttggtta atcggtattt ttcctgataa taggctgagg cgcgtttttg tgccgtttca
    13561 agaaaatatt cagggatatc ggtttcgacg accaagcttt gtcgatctga ggattgtgcc
    13621 cgagccacct tcatcgcttc gttgtatcga tgaagatcac tctgcgtctt cctgatcaaa
    13681 tgctcaaggt cacggcctaa ttcaggatcg ttatagcgtg tgtttggtag aagttggtag
    13741 gctttttgca gtaattccaa cttcattgtg taacgttttg gttcagcaga aaagaccgtc
    13801 gctatggcca ttaacaaatt aaactgttct tcaggaatga gggctaattc ggtcaggtcg
    13861 ttctctctta caaatgctaa acctaacgga ccaaacgctt cctcctgacg tagatggagg
    13921 gtaaggagat agttgagata agcgcgagcg tcttcttgtg tcatagtgaa tccttatttt
    13981 acgggacggc attttaaggt aagtttgtca ttggagcaag gtatcgtgtg aagtttatgc
    14041 caatttgagg aatccccttt tattttgatg acaatgagaa gcaacaagtt tgagctaaca
    14101 agaaaacagc gcgttcggaa gaaaggattc aatatgcaac aagacttttt gcaaaaaccc
    14161 agaggtgccg tccttatcct gggatggatt atcgcctgtc tattcctggg aatcggcgtg
    14221 acgtttgtct cggcggaaga ttcagcggtc aagaaaccag gaccggtcgc agctggagga
    14281 gaggattttt ccactgaaaa tctgaataaa caaattactg atttagaaac acaaattcag
    14341 aagctccgcg atcaaagcat cgagctacag gaaaaaactc gagccaaact tcaagcccaa
    14401 ctcgataatt tcaaaaagca acaagatacc cttattcctc gtattgagca actccgtgac
    14461 aatagtgaaa cagcttggca ggacatcaag gaaaacattc aaaaggccat tgaagatctc
    14521 aagctttccg tcgactccat gaaaaagtag agatgtgccg tgctctcagt ttatctccac
    14581 tttggcagta catcacttgc cagggacctt tcgcttttat atccttcctt tacatccttc
    14641 ttgttccctt ttttatagcc tccgctaaat ttgaaaatgg cattgatagg aatgtctttt
    14701 taccctgatc ccctatgaga gtatccccca tggtccaaca cacggaacag gtcctggaat
    14761 ggcctgtttt aattgattgc cttgccaagg aagcctcctc ctcgttaggg gagggctgct
    14821 gtcgtaccct cgcctttgca tctgaccttc attctgcagt tattcaacaa caagaaacat
    14881 cagaaatggt tcacattctc cagggccctc atcccctgcc ttctttggtg ttccccgata
    14941 tccggaatgt gttggcacgg gctcaaaaag aaggaattct ggaggggccg gaactgcgag
    15001 atattagttt ggtcttaggt cttgggcata caatcaaaga atacttgcag attcatggct
    15061 ccacgttccc gaagatcaga actcgttcct tgaaccttca agatctgata tgggtgaagc
    15121 aaacaattga ttcatgcatc gatctcaacg gccagctgcg cgagtctgcg agcccggaac
    15181 tctatcaatt ggtacgaaaa agtcagggtc ttcgtcaaac gattcggcga cgattggaaa
    15241 cgatattagc ttctcaagaa tatgaggatc ttcttcaagg acagtacttt gctgaacgtg
    15301 agaatcgata tgtgattccg ataaaagctg aacgacaaca ggctgtcaat ggaattgttc
    15361 acgatatttc cagtagtagg gccacggtgt ttgttgagcc ccgccatttg gtcgaattga
    15421 ataattctat taaatttgtt gacttgcagg tggctcagga agaacggcat ctactgcaag
    15481 atttgtccag ccgtgtggca gaacatgtct cagccattca tggaaatttg tcctgcctgg
    15541 ccgatcttga ttgcctcatg gccaaagcac gattaagcat caaaacgcat ggctctccca
    15601 ttcttcttaa ccaagaacat tgcattgact tgcaacaggc cagacatcca ctcttggcgt
    15661 tgaccaagga gcaggtcata cccaattcca ttcggattga agacgagacc aaggttctca
    15721 ttatttctgg accgaatgcg ggtggaaaaa cggtgtcatt gaaactggta ggattgttcg
    15781 cgatgatggt gaaagtcggt cttcacccaa cttgtggccc agactccaag atggcacttt
    15841 ttgagcaggt gtatgcggac attggagatt cccaggatct cagaaacgat gtatccagct
    15901 tttccgggca tattctcaat atgatcgcct tattaaaaaa tgtggaatcg tctggggaac
    15961 caacgaaccg ggatgcgctt gtacttctgg atgaagtggg cagctcgacg gatcctattg
    16021 agggtgccgc attggccgaa gcactcttaa cccgtctttg cgaattagga tgtacgattg
    16081 tcgcgaccac tcattatcca agcttgaaaa cccttccctt taggaatcct catgttcgga
    16141 atgccagcca ggaatttgat atggttactc tcgctccgac ataccggctc atagacggaa
    16201 ttcccggagg gtcttccgcc ttggatattg cggaacgttt agggttggat tcgacaataa
    16261 ttcagtctgc caaatcttta attcgagggc aggatttcga tcttgatgcc atcttccgaa
    16321 ctctacagaa cacgcacact cgattggaac gtgaatgtga acaggctcaa tatcttcgtc
    16381 aggaagcgca acgcttgctt gaggaggcgc aagtaacgcg caaccaattg aaggcgcaag
    16441 aacaggaaga tcgccaacga tatcgaaagc aatggcaaca agaattttcc aaagcacaac
    16501 ggcatctgaa tcaaatcgtg aacgacctaa aaaaggacaa aactcttttg aaggtgaagt
    16561 ctattcagca aaccataggc acattgaatc aggaaatact ttctcacctc ccaaataatc
    16621 aatgggtatc atctgagcca ctgcatgaag gggacctggt agaaattaat gcgctgggga
    16681 ctataggggt cttgcaggaa agtcttgagg ggaaaagaca ggtatccatt cgtgtgggta
    16741 cccggacaat taaaacagcc ccttctgcca tacgtcgagc gataccctct tccgacaata
    16801 aacccttgtc gggtcaacag caattgagtg ggaagcgggc tctccccaca atttccacgg
    16861 agttggcttc cccagcctcc aaccaggaaa caggcctcta tgaacaggaa ttagttgttc
    16921 gtgggttccg ttttgacgat gcgatggaga tgacgcttgc ggccctcgac catgccttgg
    16981 tcacccaagc caaatatctt aaggtcattc atggccaggg ctctggcgca ctcaaaacag
    17041 gaattcgaac tctttgtcaa tcctctccct atatccaaag ctttcgagcg ggtgatccct
    17101 ccgagggagg agacggtgtg acggtgatcg aattacgata aaggaattcg tggagcgtga
    17161 ttcttgagtc caatctgtac atttcggata aatccctcgt gcttgggtcg gcgcaagggg
    17221 ctcccttttg ttagcgaatg gaattcatct tttgtcagat tggccaatga aggtaattgc
    17281 gggtgtaatg tccagggaaa cggttggaat cctgcctcag tagtgggatt tgcatttacg
    17341 ttaaacggac aaatatccaa acaatcgtca caaccaaata ttctattgcc tatttttttt
    17401 ctcagctctg ctggaagatc atccacacta ccacggtatt caatcgtcaa atacgagatg
    17461 catcgttccg catcaaggag ata
//
LOCUS       DAORAQ010000047        17262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_577_length_17262_cov_65.931016, whole genome shotgun sequence.
ACCESSION   DAORAQ010000047 DAORAQ010000000
VERSION     DAORAQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 17262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17262
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_577_length_17262_cov_65.931016"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PKM72_06330"
     CDS             complement(<1..1571)
                     /locus_tag="PKM72_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNP60438.1"
                     /translation="MTTIQLKQEIRSEANKRRTFAIISHPDAGKTTLTEKLLLYSGLV
                     RTAGMVGGRKNRKMATSDWMEMEQERGISITASAMQFPYKRAVINVLDTPGHQDFCED
                     TYRTLTAADSAIMVIDAAKGVETQTRKLFEVCRLRQIPVLTFINKMDLPGRAPLDLMA
                     EVEDVLGIHASPINWPIGSGQDFLGVVNRQTRKVLLYTKTAAGGASKPDLMELSLDDP
                     SVKNRMAGYIWDELMHDLELLDIAGNPFSKDEFQNGKVTPVFFGSAMTNFGVEAFFDA
                     FVEFAPSAHGRLADTPDGREMMIDPVETPFSAYVFKLQANMNPRHRDSTAFLRVCSGR
                     FERDVVVKHHRTNTEVRLSRPHSMVAKERNTVDVAYPGDVIGIINPGIFQIGDTISVA
                     DAFNYKPLPQFQPEVFARVRPTDTTMRKSFDKGIEQLAREGTIQILRSLDGLESFVAA
                     VGKLQFDVLEFRLQSEYRVKVVVDVLPYESSAWLVGDVETFKPPSDALVVKDSRDRAV
                     VLFRSSWSKNFAAER"
     gene            complement(1602..2951)
                     /locus_tag="PKM72_06335"
     CDS             complement(1602..2951)
                     /locus_tag="PKM72_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP60439.1"
                     /translation="MDMMVGQVSKVVLLFVVSIGVSGGSSPVTWATPLESSTARDLPE
                     LRLSLREAMEAAVDQNPTVRLFKERIAQAQDRANTQLGELLPNLSGTAGAARRRFFTG
                     SFGSNATVVGPRDFYEMRAFLTQNVFSLSLIQKWRAAKAGVDVAGLDAEVTKRDTMAT
                     TGLIYLETLRAKAAVNARMADVALNKELLRLAAERKSAGMATSLDVTRAKVQLENARQ
                     RLLVSENDRDRAKLNLIRAMGLSFDVQLVLTDVMKLENVSEQSVGEALQVAKENRTEL
                     KAQQNRERLASLTLSSVTNERVPSIQALGDVGMIGNQIPNALTTDNVQVLMRVPIFDG
                     GMREARISESRSLVRQEAIRTQDVQYQVGLEVRDALITLSSAKQQVAVAEEGLKFSLT
                     ELELSRERFAVGVATNLEVTDAQTRVAQARDNLIEGLFTFNASRLSLARAQGQLEKL"
     gene            complement(2968..6222)
                     /locus_tag="PKM72_06340"
     CDS             complement(2968..6222)
                     /locus_tag="PKM72_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP60440.1"
                     /translation="MNLVHSAIRYPVSTAVGVLLVVLFGIVALVKLPVQLAPDVEKHE
                     VTVDTMWPGGSPHEVEREIIDEQEEQLKGVEGLEKMFSESSYGQGKVILRFPTGTNTD
                     TALLQVSNRLNQVKEYPLEADEPVISSADTRGAAMAWFILDTLEGNPVNIETMRDFAE
                     DIIKARFERVKGVAASNVYGGRERELRVTVDPAKLASRALTIMDLARALDQENRDYSA
                     GDFDEGKRSYVVRTVGEYLNPQDVENVIIARRDGASVYVRDVATAHIDYKDPTVVVRQ
                     KGHPAIAINAIRQTGANVLEAMDGLREAALELNEETLNARGFQLFQVYDETIYINRSL
                     SLVQQNLIVGSVLAISILYLFLRTGSSTLVVGLAIPISMMGTFIMLWALGRNFNVISL
                     AGMTFAAGMLVDNSIVVLENIYRHRELGKSLFQSAYDGTVEVWGAVLASTLTTIAVFV
                     PIVFMEEQSGQLFRDIAIAISAGVGLSLVVSMTVIPSLSARVLKTVKKSKKREASTVW
                     HPSTLAEKGLSERRPSGLDRIKEALGSLVYDLSGSVFWRSVTILGFTSLAMVGSWALL
                     PKAEYLPQGNRNLVIGILLPPPGYNANEFVKMGKPIESTLGPYWDAKPGSPEEAALDG
                     PSIANFFYVARGRSVFMGGRTNDPDRVKELIPLFRRATADLPGVIGIITQRGLFERGL
                     GEGRNIDIEITGPELDTLVGLGIQVFGQVKGVLPEAQVRPIPSLDLGSPEVRVTLKRD
                     RAADVQMTNQELGFTIDALVDGAKASDYQFEGDEVDLTIRGVDRYANQTQSLNSIPIY
                     TKGGQLTTVGNIADVTLVSGPEQINHIERERSIVIQVIPPVEMALEEAMDVIRTKILG
                     PLKESGTLGRLYNVRLSGTADDLTETYEALKWNFLLAFTITYLLMAALFESFLYPLVI
                     IFTVPFAAAGGFLGLSLVNNFIAYQPLDVLTMLGFVILIGVVVNNAILVVHQALNFMK
                     GRDQTDSIEEELWQGLPLREAIRESVKIRVRPIMMTTLTTLFGLFPLVVSSGAGSELY
                     RGIGSVVLGGLLVSTVFTLIVIPALFTLVIDTQRKWAVKRKPQLVAQQPSGGSL"
     gene            complement(6219..7325)
                     /locus_tag="PKM72_06345"
     CDS             complement(6219..7325)
                     /locus_tag="PKM72_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP60441.1"
                     /translation="MTRLPKSPIWLVFGLILAVQGLQGCSEQEAAQPPTGRPPSPVKV
                     VTVLSKQVQRSVSLVGTAEPRKRSLVASEVAGLVKAFPGKEGQFVKTGQLLASLRTDT
                     LDIRLDSAVASHRESKTRHEQAQRDLDRSKMLFAKELVTQKEMDDAVVEESALEKRLI
                     QLEAEIRLVRDQVTKSRILAPFSGWITKEYTEIGQWVEEGGPVVELVDLSQVEVQVPL
                     PEEYVRDVRVGDPVMAVFDALPGDEVEGTVFSVVAQADRSARTFPVKVVLANPDLRIK
                     SGMVARVKLAVGAPYQAVVIPKDALVLKGGKEFAFIVDNNTVTQVTVIPIAHFEDVVE
                     VQGAIEEGMQIVVEGNERLLPGQSVRILEEPTET"
     gene            complement(7330..7980)
                     /locus_tag="PKM72_06350"
     CDS             complement(7330..7980)
                     /locus_tag="PKM72_06350"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNP60442.1"
                     /translation="MKVISNPRAQRKQREYEARREEILLAAERKFSQNGFFKTSMAEI
                     AEDAQFAMGTVYRFFKSKEDIYISLVEAKVEDLLRLLEEATQSRLPAQEKLRAVIQVK
                     LAFADQNRAFFRIYVSEWSGFEWTVKSAFGERVWKRYLAQIDLVANLIKEGIKTGEFR
                     KVNPKDTSLAFHGMLNSTIYLWILQSGPKESLVDKGEWLGSLLLNGLGNSTLKRSI"
     gene            complement(8573..9073)
                     /locus_tag="PKM72_06355"
     CDS             complement(8573..9073)
                     /locus_tag="PKM72_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60443.1"
                     /translation="MMTKEIQVVKIGLGLVLLGLMFGVGMGILFGINEDLYKDYIKQG
                     IEANPAVHDAKSADKIWRYAQRAHFHATGIAAFSLGLIILTMLSGMKSRYKKISAIFL
                     GLSSLYPLSWLTMFLLAPSIGRGPAHGHFLTEIFVYTGVGGLLIGVVLLCGNLFLGYF
                     REETKL"
     gene            complement(9265..10614)
                     /locus_tag="PKM72_06360"
     CDS             complement(9265..10614)
                     /locus_tag="PKM72_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNP60444.1"
                     /translation="MKRNTLALRLLTLSSVWVVLTLVVVGVLLILLFRSHVERRFDDF
                     LFDQLKGNIAASDISTKSGVLEMTWTPSNLRFHRPLSGWYWQILENGKLVARSRSLWQ
                     HTLEVVDPGIGTGLKHQALTGPAGMPLRGLVENVTLPDSNASFTFVVAGPVSNIDQDV
                     HEFSKMLLVTLIALGVGLVGAVFFQIRIGLRPLSRLQQALAETRLGKTARLPESFPGE
                     VQPVVSELNALLDHNAALLDRARTQTANLAHALKNPLTVLTNEARTMEGEQGSLLGEQ
                     LKNMTHSVHRYLSRARAAGANGFVGIRTPVGTIAEDLRFSMDHLYKGKALNIHVIGLE
                     EIYFHGDVQDLEEMLGNLIDNACKWAKKEVQIRGERAGDKWRVIVEDDGPGIPEGKEA
                     VVIQRGRRLDEAIPGSGLGLDIVFDIVMLYGGSLYLDRSTLGGVRANLEWPAVDELL"
     gene            complement(10604..11275)
                     /locus_tag="PKM72_06365"
     CDS             complement(10604..11275)
                     /locus_tag="PKM72_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP60445.1"
                     /translation="MRVLVVEDNSQVARQIVDALQRAAYVVDVAPDGEQGEFYGQTES
                     YDAVILDLGLPKCEGLTVLQRWRQAGRTMPILILTARDTWREKVLGLRAGADDYLTKP
                     FEIEEVVARIEALIRRAAGHANAVLTWGSVSLDTSSQRVTIHGHPVELTALEFRTLSY
                     FLHHPHAIISKTELTEHIYNQDFDLDSNVIEVLINRLRRKLGSSFISTHRGKGYQLTE
                     FAHET"
     gene            complement(11304..11651)
                     /locus_tag="PKM72_06370"
     CDS             complement(11304..11651)
                     /locus_tag="PKM72_06370"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNP60446.1"
                     /translation="MNLDRQKISKNDRIALPWIVGVMIGLFGFLPPAYPDDDIPGYQQ
                     LKAEVVPVEQLFQKVQKEFEGIILELELEEENLRWIYEVKLLTPQGNVLKIDYDAKTM
                     AVLMVKGQRDPSP"
     gene            complement(12091..>12880)
                     /locus_tag="PKM72_06375"
     CDS             complement(12091..>12880)
                     /locus_tag="PKM72_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60447.1"
                     /translation="GMNNSLIAVWFLLLMTISNIIHASERGTYSDSPRLPEPMVFDLV
                     LPLGAHKNEYEFNALSQYNFKDDAMRFNPEFEYAFADGYGIEFELPMQNGVVKAYKLG
                     LQGTFDFLRNSRFIHGWQYIGEYHRHLAQFEDASGEFEDDPGEFKGGGGFENDLLYVF
                     GYQFNRHWSMLNMTGLRLTDIGSHGHLEGLVNGNLTYSFSKKNHMGLEMNWEFRPKRP
                     DLMLVMPQLHVRLAKHAKIQFGFGMERTEHQNFSVAAARVIFGF"
     assembly_gap    12881..12980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13252..13707
                     /locus_tag="PKM72_06380"
     CDS             13252..13707
                     /locus_tag="PKM72_06380"
                     /inference="COORDINATES: protein motif:HMM:NF033674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HNP60448.1"
                     /translation="MSKHIIFMTVGFLLFSTLTLWAKFQGPGTPGITSISAAQEAVDD
                     SWVVLSGTILRQVGEERYLFRDPTGTMIVEIDHEDWGNVTANPETILRVSGEIDRAWG
                     TTEIEVKQIEMEIVSSPDLSRLQSDHSVPTNPIDGMPTSDQSVTLHQLH"
     gene            13952..14173
                     /locus_tag="PKM72_06385"
     CDS             13952..14173
                     /locus_tag="PKM72_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60449.1"
                     /translation="MNHLYMPGETHAWYTPGQIFTWLQEQRYQVFSILYDDQWYVVEA
                     RDSDGTWVELKVTPKTRTIFAWKFFEEPC"
     gene            complement(14514..15104)
                     /locus_tag="PKM72_06390"
     CDS             complement(14514..15104)
                     /locus_tag="PKM72_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNP60450.1"
                     /translation="MELQRPKFWDNINVNDLARNFFEGLLILVPVVTTLYVAWLVLQT
                     IDGWLNIPIPGVGFLVTVGLITLTGRYASTVFVQKMLDVLERVLVKAPFVKILYTSLK
                     DLIAAFMGEKRRFDQPVVVTLSPGGYAEVVGFVTRTDLEFLGLLDHVAVYFPQSYNFA
                     GHVLVFPKMQVRPLEAESADVMAFILSGGVSGGENR"
     gene            15356..15610
                     /locus_tag="PKM72_06395"
     CDS             15356..15610
                     /locus_tag="PKM72_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60451.1"
                     /translation="MAQSSSGTTPAATDSQETEILYVVIQRDGKQVSAKWGLHPNLKE
                     ELKPEEWKELTEVMGKVTTLVGGRFSQVLNKAEEESGGTA"
     gene            complement(16063..17049)
                     /locus_tag="PKM72_06400"
     CDS             complement(16063..17049)
                     /locus_tag="PKM72_06400"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNP60452.1"
                     /translation="MKSLVIGGTGQLGANLVRVLLERGDQVRVLHRSTSNTFTIDGLN
                     VERVVGDLSDGESLRLACEGRDMVYHTAGYYPSGAIPVEMAKGQALKETALVLAAVRA
                     ARVDRLVFASTLTTVGFPKTVGHPANETCQFSTRYTNNPYLMAKAAMEEKILQSVHQG
                     IPAVVVIPTEFFGPYDQHPTSGTHILMVAKGRMPVYVPGRVNVIDVRDVAVAMIRAAE
                     RGRVGERYLVGHWNTTQKDLNELIAQEIGVQPPFFPVPLALARWGAKVVEWVSRSLLR
                     RPPFVPAFFVEVMAHMQHYDCSKAQRELDYPQSATQGAIKDAVTWFRENGYL"
BASE COUNT         4501 a         4759 c         3849 g         4053 t
ORIGIN      
        1 ttgcgttcgg cggcgaagtt tttcgaccac gagctgcgaa acaggacgac ggcccggtcc
       61 cggctgtctt tgacgacaag agcatctgat ggtggtttaa atgtctccac atctcccacc
      121 aaccaggcac tggattcata gggcaacaca tccaccacca ctttgacgcg atattcgctt
      181 tgtaaacgaa attccaatac atcaaactgg agtttcccga cggcagctac aaaagattcc
      241 aggccatcca gactccgaag gatttgaatg gtcccttccc tggcgagttg ctcgatgcct
      301 ttgtcgaacg acttccgcat ggtggtatca gtggggcgaa cccgagcaaa gacttccggt
      361 tgaaattgtg gaagcggttt gtaattaaac gcgtccgcca ccgaaatcgt gtcgccaatc
      421 tgaaaaatcc ctggattgat aatcccaatg acatcaccgg gataggccac atcgaccgta
      481 ttgcgttcct tcgccaccat actatggggc cttgagagcc gcacctccgt gttggtccga
      541 tgatgtttca ccaccacatc ccgctcaaat cgcccggaac acacccgtaa aaaggccgta
      601 ctatcgcgat ggcgtggatt catattggcc tggagtttaa acacataggc gctgaatggg
      661 gtctccaccg gatcgatcat catttcacgg ccatccgggg tatccgccaa tcgaccgtga
      721 gcggaaggcg cgaattccac aaacgcatcg aaaaatgctt ccacgccgaa attggtcata
      781 gctgatccaa agaagaccgg cgtgactttt ccattttgaa actcatcttt cgaaaacggg
      841 ttcccggcaa tatccagcaa ttccaaatca tgcatcaatt catcccagat atacccggcc
      901 atccggtttt tcactgaggg gtcgtccaac gagagttcca tgaggtccgg tttcgacgcc
      961 cctcccgcag ccgttttggt atagagcaac acctttctgg tttgccgatt cacgaccccg
     1021 agaaagtctt gtccggaccc gatgggccag ttgatgggac tggcatgaat gcccaatacg
     1081 tcttccactt ctgccatgag atccagcggg gcccgtcccg gcaagtccat cttgttaatg
     1141 aaggtcaata cgggaatctg gcgaagacgg cagacttcaa acaattttcg tgtttgcgtt
     1201 tcaacgccct tggcggcgtc aatgaccatg atcgcgctat cggccgccgt gagtgtccgg
     1261 taagtatcct cgcaaaaatc ctgatggcct ggcgtatcca acacgttgat aaccgcccgt
     1321 ttatagggaa attgcatcgc cgaggcggtg atggaaattc cgcgctcctg ctccatttcc
     1381 atccaatccg acgtggccat tttccggttt ttccgccccc ctaccatacc ggcggtgcgc
     1441 accagccctg agtagagcag gagtttttcg gttaaggtgg ttttgccggc gtccggatgg
     1501 ctgatgatgg caaaggtccg acgcttattc gcttctgacc ggatttcttg tttgagttgg
     1561 atggtcgtca tattaccttt tgggcgaata ggcccgatct attataattt ctccaactga
     1621 ccctgcgctc gggccaggct caggcgagag gcattaaagg tgaagaggcc ttcaatcaaa
     1681 ttatctctgg cttgtgccac acgggtttgc gcatcggtga cttcgagatt ggtggcaaca
     1741 ccgaccgcaa accgttcacg cgataattcc aattccgtga gagaaaattt caaaccttct
     1801 tccgccaccg ccacttgctg tttggccgaa ctcaatgtga tcagcgcatc gcgcacttcc
     1861 aaacccacct gatattgtac atcctgtgtc ctgatggctt cctgcctgac gagactgcga
     1921 ctctctgaaa ttctggcttc ccgcattccc ccatcgaaaa tcggcaccct cattaatacc
     1981 tggacattgt cggtggtcaa cgcattgggg atttgattac caatcatgcc cacatcgccc
     2041 aaggcctgaa tggagggcac ccgttcgttg gtcacggaac ttaacgtcag ggaagccagt
     2101 cgctcacgat tctgctgtgc cttcagttca gtccggtttt cttttgccac ctgcaaggct
     2161 tctccaacgg attgttcaga gacgttttct aatttcatga catcggtaag caccaactgc
     2221 acatcgaacg acaaccccat cgcccggatg agattaagtt tggcacgatc ccggtcattt
     2281 tcagagacca ataatcgctg tctggcgttt tccaattgaa ccttcgccct cgtcacatca
     2341 aggctggtcg ccatgcccgc ggattttcgt tcggccgcca aacgcagtaa ttccttattc
     2401 agcgccacat ccgccatgcg agcattcact gccgctttgg ctcgaagcgt ttccaaataa
     2461 atcagacctg tcgtcgccat ggtatcccgt ttagtgactt ctgcatccaa tcctgccaca
     2521 tcgactcccg ccttggcagc ccgccatttt tggatgagac tcaaactgaa cacattctgg
     2581 gtgagaaagg cccgcatttc gtaaaaatcc ctgggaccga cgacggtggc attcgagcca
     2641 aaggaccctg taaagaatcg acgccgggcg gcgcctgccg ttcctgaaag attgggcaat
     2701 aattcgccaa gttgagtatt ggctctatcc tgcgcctgtg caatacgttc tttaaacaac
     2761 cggaccgtgg gattttgatc gacggcggcc tccatggctt cccgcaaact caaccgcaac
     2821 tcgggaagat cccgagccgt ggaagattcc agcggagtag cccacgtcac cgggctggat
     2881 ccgcccgaca ctccgatgct caccacaaat agcaacacca ccttcgaaac ctgtcctacc
     2941 atcatatcca ttcccttcgc ctcttgacta cagagagcct cccgagggtt gttgagccac
     3001 caattgaggc tttcttttca ctgcccattt gcgttgcgta tcaatgacca aagtaaacaa
     3061 agccggaatg acaataagag tgaacaccgt ggagaccaag aggccaccca ggaccacact
     3121 cccgatcccc cggtacagct cggatcccgc gccagacgag accactagcg gaaaaaggcc
     3181 aaagagcgtc gtgagtgtcg tcatcataat cggccggacg cggattttga cggattcacg
     3241 aatcgcctcc cgaagcggta atccttgcca caattcttct tcaatcgaat ccgtctggtc
     3301 ccgtcccttc atgaaattta aggcctggtg caccaccaga atggcattat taactaccac
     3361 cccgatgaga atgacaaaac ctaacatcgt caacacatcc aatggctggt aggcaataaa
     3421 attattgacc agagaaagcc ccagaaaacc accggctgcg gcaaacggca ccgtaaatat
     3481 aatcaccagg ggatagagaa aactttcgaa caaggcagcc atcaacaaat aagtaatcgt
     3541 aaaggccaag agaaaattcc attttaacgc ctcataggtt tcggtgagat catcagccgt
     3601 ccccgacaac cggacattat acaaacgtcc taacgtccca ctttctttca atggcccaag
     3661 gattttcgtt cgaatgacat ccatcgcctc ctccagcgcc atctcaacgg gcgggatcac
     3721 ctgaataaca atggatcgtt cccgttcaat atgattaatt tgttccggcc cggagactaa
     3781 tgtgacatcg gcaatattgc cgaccgtcgt caactgcccg cctttggtat aaatcggaat
     3841 agaattgagc gactgcgtct gattggcata ccgatcgacc ccacgaatcg tcagatcaac
     3901 ttcatcgccc tcaaattggt agtcgctggc ctttgcccca tcgaccaggg cgtctattgt
     3961 aaatccgagt tcctgattgg tcatctgcac atcggctgcc cgatcccgtt tcagggtgac
     4021 acggacttcc gggcttccca aatccaaact gggaataggc ctgacctgcg cctcgggcag
     4081 cacccctttt acctgaccga agacttgaat acccaacccc accagcgtat ccagctccgg
     4141 cccggttatt tcaatatcaa tatttcgtcc ctcgcccagg ccacgttcaa acaggccgcg
     4201 ttgggtgata ataccaatca cgccgggaag atcggccgtg gctcggcgaa atagagggat
     4261 caattctttg actcggtcgg gatcattcgt ccgaccaccc ataaagaccg atcgcccacg
     4321 cgccacatag aaaaaattgg caatgctggg tccatccaag gcagcttctt ccggggaacc
     4381 aggcttcgcg tcccaatacg ggcctaaggt cgattcaatc ggctttccca ttttgacaaa
     4441 ttcgttggcg ttataacccg gcggaggcag cagaataccg atgaccaggt tccggtttcc
     4501 ctgaggaaga tattccgcct tcggcaacaa agcccagctt cccaccatgg caagcgaggt
     4561 gaaccccaaa atggtcacac ttcgccagaa cacacttcca ctgaggtcat agacgagaga
     4621 tcccaatgcc tctttgatac ggtccaaccc ggaaggccgt ctttccgaca agcccttttc
     4681 cgccaatgtt gatggatgcc aaacagtgga agcttctctt tttttggatt ttttgaccgt
     4741 ctttaagact ctggcagata acgaaggaat cacggtcatt gacaccacca ggctcaagcc
     4801 cacacccgca ctaatcgcaa tggcaatgtc tcggaataac tggcccgatt gttcttccat
     4861 aaacacaatg ggcacaaata ccgcaatcgt ggtgagggta ctggccagca ctgcccccca
     4921 aacctcgaca gtgccatcat aggcagattg aaataacgat tttcccaatt cccggtgacg
     4981 ataaatattt tccagcacca caatcgagtt atccaccaac ataccggcag cgaacgtcat
     5041 tcccgctaaa ctgatgacat tgaaattccg gcccaacgcc cacagcataa taaacgttcc
     5101 catcatgctg atcggaatgg ccagaccgac caccagggtg ctactgcccg tgcggaggaa
     5161 tagatacagg atggagatcg ctaacacact cccgacgatt aaattttgct ggacaagaga
     5221 tagtgaacgg ttaatgtaaa tggtttcatc atagacctga aataactgaa accctcgtgc
     5281 attcaacgtt tcttcattga gctcaagagc agcctcgcgc agtccgtcca tggcttccaa
     5341 cacattcgcg ccggtctgcc gaatcgcgtt gatcgcgatg gcgggatggc ctttttgtcg
     5401 caccacaacg gttggatcct tgtaatcaat atgggcggtc gccacatccc gcacatacac
     5461 cgacgcgcca tcacgcctgg caatgatgac attttccaca tcctgggggt taagatattc
     5521 tcccaccgta cgtacgacat acgaccgctt cccctcatcg aaatccccgg cactgtaatc
     5581 ccgattctcc tgatctaacg ctcgagccag atccataatg gttaacgctc tggacgctaa
     5641 tttagccgga tcgaccgtca cccgaagctc ccgctcccgc ccgccgtaga cattcgaagc
     5701 ggcgacaccc ttgacccgtt cgaaacgggc cttgatgata tcctctgcaa aatcccgcat
     5761 ggtttcaata ttgacgggat taccttccaa ggtgtctaaa ataaaccagg ccatggcggc
     5821 cccacgggta tccgcactgc taatgacggg ttcatccgcc tcaagcggat actccttgac
     5881 ctgattcaac cggttggaga cttggagtaa ggccgtgtcg gtattcgttc ctgtgggaaa
     5941 tcgaagaatg acttttccct gaccatagga actttcgctg aacattttct ccaggccttc
     6001 gacacctttg agttgctctt cctgctcatc aatgatttcc cgctcgactt catgcgggct
     6061 tcccccaggc cacatggtat ccacggtgac ttcatgtttt tccacatcgg gtgccaattg
     6121 aaccgggagc ttcaccaacg ccacaatgcc gaacaacacc actaagagca cacccactgc
     6181 ggtgctgacc ggataacgga tagcggaatg aaccagattc atgtttctgt cggctcctct
     6241 aatatcctga ccgattgacc gggcaggagg cgctcattcc cttccacgac tatctgcatg
     6301 ccttcttcaa tagctccctg tacttcaacc acgtcctcaa aatgggctat gggaatcacc
     6361 gtgacctgcg tgacggtatt attatcaaca ataaaggcga attccttgcc ccctttgagc
     6421 accaacgcat ccttgggtat cacgaccgcc tggtaaggag cgcccacggc taacttcacc
     6481 cgtgcgacca tcccgctttt aatgcgtaaa tccggattgg ccagcacaac cttgaccgga
     6541 aatgtccgtg ccgaccgatc ggcttgcgcg acgacggaaa agactgttcc ttcgacctca
     6601 tccccaggca aggcatcgaa caccgccatc actggatccc cgaccctgac atcccgcaca
     6661 tattcttccg gaagcggcac ttgcacctcg acctgagaca ggtccaccaa ttccaccacc
     6721 ggtccaccct cttcgaccca ttggccgatt tccgtatatt ctttcgtgat ccaaccggaa
     6781 aaaggcgcca gaatccttga tttggtcact tgatcacgta ccagtcgaat ttccgcttct
     6841 aattgaatca gccgcttttc tagagcgctt tcttcaacaa ccgcatcgtc catttctttt
     6901 tgagttacca attccttggc aaacaacatc ttgcttcggt ccaaatccct ctgggcctgt
     6961 tcatggcgag ttttggattc acgatgcgaa gccactgccg agtcaagacg aatgtccagg
     7021 gtgtctgttc gaagactggc gagcagttgt cctgtcttaa caaactgacc ttccttaccg
     7081 ggaaaggcct tgaccaggcc tgccacttca ctggccacaa ggcttcgctt gcgtggctcc
     7141 gctgttccca ccaatgaaac cgatcgctga acctgcttac tcaaaacggt cacgaccttg
     7201 accggcgagg gcggacgtcc cgttggcggc tgggcggcct cctgttcgct gcacccctgc
     7261 aacccctgca cagccagaat caggccaaac accaaccaaa ttgggctttt aggcagacgt
     7321 gtcatacgat tatattgacc tttttaatgt tgaattcccg aggccgttaa gcaacaagga
     7381 ccctaaccac tcgcctttgt ccacaagtga ttctttcggg cctgactgga gaatccaaag
     7441 atagatggtg gagtttaaca tgccatgaaa ggccaaagag gtatctttgg gattcacttt
     7501 gcggaattct ccggtcttga taccctcctt gatgagattc gcgacgagat caatttgcgc
     7561 caagtaccgt ttccacaccc gctcgccaaa cgcgcttttc accgtccatt caaacccgct
     7621 ccactcggaa acataaatac ggaagaacgc acggttttga tcagcgaagg ctaactttac
     7681 ctgaatcact gcacgtaact tttcctgggc agggagacgg ctttgcgtcg cttcttccaa
     7741 aaggcggagt aaatcctcca ccttggcttc caccagagag atataaatgt cttctttgct
     7801 cttaaaaaac cggtacaccg tccccatggc aaattgagca tcctccgcaa tctcggccat
     7861 gctggttttg aaaaacccgt tctgagaaaa ctttcgctca gcagctaaga gtatttcctc
     7921 ccgccgggct tcatattccc gctgctttcg ttgtgcccga ggattggaaa tcactttcat
     7981 aaatgacctt ttactgaaaa agttctagcc ggctttgaat acctattcta aaatatgaat
     8041 actcattcca caacatgaca ttctgattcc attccttttc ggtcctattg gtccttttct
     8101 gataggaaaa atttaccggg aatcctattt cctgagtaga actctgattt gagctacctt
     8161 agggtcactt tacagccatt tgccctatca ctgagcagag gaagggtagc ccaagatctg
     8221 atccaccccc ctctcattcc ttaatcattc atgcatctat tttaatagga gaaaaagaga
     8281 aggataacac ccgggcagaa cgccgaaatc tccggccaat ctctttccag gtgtggaaga
     8341 aatatgcctg gagtgacaag atagggaaga tgatgtgaga agaccggctc cctcaaattc
     8401 tcgggagtcc tacaaaaggg ggctcatggg aagatagccg aaatgagatt acagacaccc
     8461 tataacgatc tgggagggat ataggtggcc cttcctaaat tagtttagtt tttttgtact
     8521 tgcaacccac ccattcggtt ggctctgaat taaagattac ccccaccatg tcctacaact
     8581 tagtctcttc gcgaaagtat cctaaaaaca gatttccgca aagaagaaca acgcctatca
     8641 ataaacctcc cacacccgtg tacacgaata tttccgtcag aaaatggcca tgagccggac
     8701 cacgcccgat cgagggggcc aagagaaaca tggtcagcca ggaaagtgga tacaagctgc
     8761 tgaggccgag aaaaattgcg gaaatttttt tgtatctgga tttcattcca gacaacatgg
     8821 tgagtatgat cagtcccagg gaaaaggcgg caatacccgt tgcatggaag tgcgcacgtt
     8881 gcgcatatcg ccagatctta tccgcacttt tggcatcatg tactgccgga ttcgcttcaa
     8941 tgccctgttt aatataatct ttatacagat cttcattgat accgaagaga attcccattc
     9001 ccacaccaaa catcagtccc aacagcacga ggcccagtcc aattttcaca acttgaatct
     9061 ctttggtcat catgttcact tcccatctcc aatcgctctg ttataaataa tgtatgccca
     9121 tcgcacgttt cttgaaatcc atcttctact cttcacataa atacggtcat gaggatgatg
     9181 acatatgccc aatccctact tgtcagttca ccgaaagtgt gagcccgttg atttgctcag
     9241 acaggtgagc atggagcttg tggatcacaa cagctcatcc actgcgggcc attctagatt
     9301 cgcgcgcacg cctcccaacg tcgatcggtc caagtacaat gagccgccat acagcatgac
     9361 aatatcaaaa acgatgtcca accccagacc gctacccggt atggcttcat cgagcctgcg
     9421 tcctcgttga atgaccacag cttccttgcc ttcggggata ccaggaccat catcttccac
     9481 aatcacccgc catttgtccc ccgcccgctc tccacgaatc tgaacttctt ttttggccca
     9541 tttacaggcg ttgtctataa gattgcccaa catttcttct aaatcctgga catcgccatg
     9601 aaagtaaatc tcctccagcc ctatgacatg aatgttgagg gctttgccct tgtaaagatg
     9661 atccatcgaa aaacgcaaat cctcggctat ggtcccgaca ggcgttcgga tacccacaaa
     9721 tccgttcgcc cccgccgctc tagcccttga aagataccga tgcaccgagt gggtcatgtt
     9781 cttcagttgt tctcccagca gactcccctg ctctccctcc atggtacgag cctcattcgt
     9841 caggaccgtt aaaggattct tcagcgcatg cgctaaattt gccgtttgag ttcgagctcg
     9901 gtcgaggaga gccgcattat gatccagaag cgcatttaat tcggagacaa ccggttgcac
     9961 ttcccctgga aagctttccg gaagcctggc tgtttttccc aatcgggttt ccgccaaggc
    10021 ctgttgtaac cgggaaagcg ggcgtaaccc gattcgaatc tgaaaaaaca cggcacccac
    10081 taatcctacc cccaatgcga tcaacgtgac taacaacatt ttagaaaatt cgtggacatc
    10141 ctgatcaata ttggaaacgg ggcctgccac cacaaacgta aaggaggcat ttgaatcagg
    10201 cagggtgaca ttttcaacga gaccacgtag cggcattccg gcaggccccg tgagagcctg
    10261 atgtttcaaa ccggtcccaa tgccaggatc gacgacctca agggtatgct gccagaggga
    10321 gcgggagcgg gcaaccaatt tcccattttc gaggatctgc cagtaccaac cggatagggg
    10381 ccggtgaaag cgaagattgg aaggagtcca ggtcatctct aacactccgg acttggtaga
    10441 aatatcactg gccgcaatat tgcccttaag ctgatcaaac aaaaaatcat caaaacgacg
    10501 ttcaacatgg gagcgaaaga gaaggatgag cagcacaccg accaccacaa gagtcagcac
    10561 cacccacacg ctcgatagag tcagcaggcg cagggcaagc gtattacgtt tcatgagcaa
    10621 actcggtgag ttggtatccc ttaccccgat gggtgctgat aaatgaggat cctaacttcc
    10681 ttctcaatcg attgattaga acttcaatca cattagaatc caaatcaaag tcctgattgt
    10741 agatatgttc cgtgagttcg gttttagaaa tgatggcatg aggatgatgg agaaaatacg
    10801 ataaggtacg aaattccagg gcagtcaact ccacggggtg accgtgaatg gtcacccgct
    10861 ggctactcgt atcgagggaa actgaccccc aagtcaggac tgcgttggcg tggcccgcag
    10921 cccgccgaat gagagcctca atccgggcca cgacttcttc aatttcaaag ggtttggtaa
    10981 gataatcatc cgcgcccgca cgcaaaccca acactttttc ccgccaagta tccctcgcgg
    11041 ttaaaataag aatgggcatc gtgcgtcccg cttggcgcca acgttgtaac acggtcaaac
    11101 cttcacattt tggaagaccc agatcaagaa tcaccgcgtc ataggattcc gtctgaccgt
    11161 aaaactctcc ttgctcacca tcaggagcta catccacgac atacgctgct cgctgcagcg
    11221 catcgactat ttgcctggcc acttgggagt tatcttcaac aacgagtact ctcatgagat
    11281 aaacggggga cagagggtgc ttatcatggt gatggatctc gctgcccttt gaccatgaga
    11341 accgccattg ttttggcgtc gtagtcgatt ttcaaaacat tgccctgcgg ggtcagaagc
    11401 ttgacctcat aaatccagcg caaattctcc tcttctaatt ccaactcaag aatgattccc
    11461 tcaaactctt tttgcacctt ttggaacaat tgttcgacag gaaccacttc cgcctttaat
    11521 tgttgatacc cgggaatgtc gtcatccgga taagctggtg ggagaaatcc gaacaggccg
    11581 atcataaccc cgacgatcca cggtaatgca atcctatcgt ttttggatat tttctgtcga
    11641 tcaaggttca ttttctcttc ctcctttccc tttgcggcca tatgggaaaa cctctctgga
    11701 gcaaaatctt atcatgacca accttacgcc aagcttaaaa ttccgttaat cgcaaaatca
    11761 ggacataaga cctcccggat ttatcatcat tcacaaaaaa cattcccaga ggggtcaaca
    11821 cgtcatgaac ggtagtcgaa atggtgaata ttctcgcagg caagagacag caaaaacggc
    11881 catcgaggac gacaaatagg gcgtgaagaa gcccaggacc gcgacgaccc ataggtcaaa
    11941 tcgagcccac cgatacgaag gtttccctct gacataactt tcattttttc ccatccagcc
    12001 cccctcctca tcatccctcc ggaatatacg ttgaggagtt tcaggaaggt tttatcgatt
    12061 tgggcattct atgagtgcat aaacaatgga tcaaaatccg aaaataaccc gtgcagcagc
    12121 aactgaaaaa ttctgatgtt ctgttctttc catcccgaat ccgaattgta tttttgcatg
    12181 tttggctaaa cgaacatgca gttggggcat cactaacatt aaatcagggc gttttggtcg
    12241 aaattcccaa ttcatctcta atcccatatg attttttttc gagaatgaat aagtaaggtt
    12301 gccattcacc aatccctcaa gatgcccgtg agacccgatg tccgtcagac gcaatcccgt
    12361 catattcaac atactccaat gccgattgaa ctgatatcca aatacgtaga gcagatcatt
    12421 ctcaaaccct ccaccacctt taaactctcc aggatcatct tcaaactctc cactcgcatc
    12481 ttcaaactgt gcaagatgcc tatggtattc cccgatgtat tgccagccgt gaataaaacg
    12541 actatttcgg agaaaatcaa aggtaccttg caatcccagt ttataggctt tcacgacacc
    12601 attttgcatc ggtaactcaa attcaatccc atagccatct gcaaaggcgt actcgaattc
    12661 aggattgaat ctcatggcat cgtctttgaa attatattga gacagggcat tgaactcgta
    12721 ctcattttta tgggctccca agggtaacac caaatcaaac accatgggct cggggagacg
    12781 aggggagtcg ctatatgttc cacgttcaga ggcgtggatt atattcgaaa tagtcatgag
    12841 aagaagaaac cagactgcaa ttaatgaatt gttcattcct nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn ccctgctcct atctagaacc gcatcctaac gatccaacat
    13021 ttaagccagg ctgaaatgca ttttcagcgt ggcttcagga tgctggaggt acaatcccaa
    13081 aagaataatg acaggcttga gacacacaga aaggaggttc agggaatagt cggagaggtc
    13141 aacggaccca tcatttggca tgtagagggt tggatgattg gttgggctat cagccgaaac
    13201 aagccttagg tgccttacac ctcaaacctt tttcaaccaa ggagaaacat tatgagcaaa
    13261 cacattatct tcatgaccgt cggatttctt ttattcagta ctctcactct ctgggcgaaa
    13321 ttccagggac caggaacgcc tggcattacg agcatatctg ccgctcaaga ggcggttgat
    13381 gattcctggg tggtgcttag cggcaccatt cttcgccaag tgggagaaga acgctacctt
    13441 tttcgggatc ctactgggac aatgatcgtc gaaatcgatc acgaagattg gggaaacgtc
    13501 accgccaacc cggaaaccat cctgcgagta tcgggagaaa tcgatcgagc atgggggacc
    13561 acggaaattg aagtgaaaca aatcgagatg gagatcgtct caagtccaga cctgagtcgg
    13621 ttgcaatcgg accattccgt tccgaccaac ccaattgacg gcatgccgac ttcagaccag
    13681 agcgtaaccc tgcatcaact gcattaagga ggaacggtct atcaagactt cacaccctcc
    13741 atcaattggt ggagggtgtg agcaacagaa ttgccggaat gttgatcctg aatgcatttt
    13801 cgtaatcagg tcccgacata gcatgtgaac ttagcagatt aaccgagcag atcttttata
    13861 acgatttaga tgatacgcat gaagatcaga accgtatggc atttttaacc atcagaagat
    13921 cctcgtagat tcagccaacc aaggaggaca tatgaaccac ctatatatgc ctggcgaaac
    13981 ccatgcctgg tatacccccg gacaaatttt tacctggctt caggaacagc gttatcaggt
    14041 ttttagtatt ctatatgatg accaatggta tgtagttgaa gctcgtgatt cggacggcac
    14101 atgggttgaa ttgaaggtca cacccaaaac cagaaccatc tttgcgtgga aattcttcga
    14161 agaaccctgc tgaaatcttt ggtagggtgg atagattgcc tagaccagct gattgaagtc
    14221 ctacagagaa tgagacggca agaggactat gcttccaaaa ttcgtgagag ccctgcttct
    14281 tagatttggc actttgcgtt ctgggaatag agtatcatga atatttcaga ggcctaacta
    14341 ggtcagagag tgatgtcgaa attcttgagt aacaaggggt aggggtgagg taatgtttga
    14401 attgaacaaa atattccttc agaaaaattg tctggttatc ctaaatcccg tcaatggaat
    14461 tactatcgaa ctcactcaca ttcttatgag gtaattttct gtgcacctac cccctaccta
    14521 ttctcccccc cggagacccc tccggacaat ataaatgcca tgacatccgc gctttccgcc
    14581 tcaagcggac gaacctgcat ttttggaaag accagcacat gcccggcaaa attataagat
    14641 tgcggaaaat agacggccac gtggtcaagc aagccgagaa attctaaatc cgttcgagtc
    14701 acaaaaccca ccacctccgc ataaccaccc ggcgaaagag tcacgaccac aggctgatcg
    14761 aagcgacgct tttcccccat aaacgccgca atcaggtcct tgagcgaggt gtagagaatt
    14821 ttgacgaagg gcgctttgac caacacccgt tccaacacat ccaacatttt ttgcacaaac
    14881 acggtggacg catatcggcc ggtcaaggtg attaacccga ccgtgaccag aaatcccaca
    14941 cccgggatgg gaatattcag ccacccatca atggtttgaa gcaccaacca cgccacatac
    15001 aacgtcgtca caaccggaac caggattaag agaccttcaa aaaaattcct ggccagatcg
    15061 ttcacattga tattgtccca aaatttgggt cgttgtaatt ccatatcaga ctacccccat
    15121 aagaaagtga ttacccgcat gcccagctca caaggttgag acatcctgaa aactccccat
    15181 tattcctggg caaaatgata aatggacaga ccgtctccca gttaaagaaa ggaccccacc
    15241 gccaaaagtc acacaaacgt cggacaatgg tcaacatgag gaattgtggg tggttacagg
    15301 gaacagggca atgcagtaca atcgcctcaa cacattctta acagggagag caggcatggc
    15361 acaaagcagt tccggaacga caccagcagc aaccgattcc caggaaacag aaatcctata
    15421 cgtcgtgatt caaagggatg gcaagcaggt cagtgccaaa tggggactcc atccaaatct
    15481 taaagaggag ttgaaacccg aagaatggaa ggagctgacc gaagtgatgg gaaaggtcac
    15541 cacactcgta ggaggccgtt tttcacaagt cttaaacaag gccgaagaag aatcaggagg
    15601 caccgcgtaa gcgagaccat ctgtcctttt gactcgagat ccggaaggtc acattcccca
    15661 cgaccggcta tatcctgatt ttctcaaacc ccttcttgct cgttttctca ggactcttag
    15721 ggcaagattc actctcagtg cctcatattg cacttctgct ggctggctct caactatcca
    15781 aagagaagag ctaagagcag cggtaacaca tcggaacatt ccactcgctc tctgttgaga
    15841 aaaatcttga aatggatatg tcggacaatg tcagatgaat tctgagcgag caacgaatga
    15901 agtcacctca gatagaaatt gtcatctgga tctcgcacag tcgtggcact tcaacccggg
    15961 cacagaccct tggccattgg atcaatatga caggcgaatt gacgtgccct gcaatcacaa
    16021 ttggcccatc caaggtctga tgatgaaatt aaaatttcaa ccctagaggt acccattttc
    16081 ccgaaaccag gtaacggcat ccttaattgc cccttgggtg gcactctgcg gataatccaa
    16141 ttctcgctga gcttttgagc aatcgtaatg ctgcatgtgc gccatcactt cgacaaaaaa
    16201 ggcaggcaca aaggggggcc ggcgtaagag agaacgtgac acccactcca cgacttttgc
    16261 tccccaacgg gcgagagcca atggaaccgg aaagaaagga ggctgcactc caatttcctg
    16321 cgcaatgagt tcattcaagt ccttttgtgt cgtattccag tgacctacga gatagcgttc
    16381 cccgacacga ccgcgttctg ccgcccggat catggccacg gccacatcgc gcacatcaat
    16441 aacattcacc cggccgggga catacaccgg catccgccct ttcgcgacca tgagaatatg
    16501 ggtgccgctt gtcgggtgct gatcgtaggg accgaaaaac tccgtcggga ttaccaccac
    16561 tgcgggaata ccctggtgaa ccgattgcag aatcttctct tccatggcgg ccttggccat
    16621 gagatacgga ttgttcgtat atcgagttga aaattgacaa gtttcattgg ctggatggcc
    16681 gaccgttttg ggaaatccca cagtcgtgag cgtactggcg aaaacgagtc ggtccactct
    16741 ggccgcacga acggctgcta acacgagtgc cgtttctttc agagcctgcc ctttggccat
    16801 ctcaactgga atcgccccag acggataata cccggcagtg tgatagacca tgtccctgcc
    16861 ctcacaggcc aagcgcaaag attctccatc actgagatca cccaccacgc gctcaacatt
    16921 tagcccgtca atcgtgaagg tgttgctggt ggatcgatgc aacacacgaa cctggtcccc
    16981 acgctcaagt agcacccgaa ccaaattcgc tccaagttgc ccggttccac cgatgaccaa
    17041 cgatttcatc tgaaacccct gtcgagtaat cgtggagaaa gtcctctatc aggtagaacg
    17101 taataccctc taatcggctg taccgttggg acccggacac atacgcatgt ttgcatgatt
    17161 gcagaaaagg aaaacaggaa aactaatagt gtttactgcc caaaaccctg aatggcgttc
    17221 cacgcatcat tgcctggtaa accaaaggag gcgggagaat ct
//
LOCUS       DAORAQ010000048        17052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_586_length_17052_cov_61.072307, whole genome shotgun sequence.
ACCESSION   DAORAQ010000048 DAORAQ010000000
VERSION     DAORAQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_17052_cov_61.072307"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKM72_06405"
     CDS             <1..317
                     /locus_tag="PKM72_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60453.1"
                     /translation="TWVGSDFIRAVLDLFVYADPETGSLVIGAGLPRHWLQGEGIRVR
                     GLKTPYGEVSYTAHDEDGQVLVDLEGSSMPPGGFVFPASLKGDLKVTFNGDPVNVHAT
                     SP"
     gene            601..1989
                     /locus_tag="PKM72_06410"
     CDS             601..1989
                     /locus_tag="PKM72_06410"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNP60454.1"
                     /translation="MNKQTTFPEGFLWGAATSAYQIEGAPLADGAGPSIWQRFSHTPG
                     HTLNGETGDVACDHYHRWREDIALMKELGLQAYRFSVAWGRIFPEGKGRLNEKGLDFY
                     DKLVDGLLEAGIEPLLTLYHWDLPEALDDLGGWLNPDIAHWFADYGEAMYRRLDGRVK
                     KWTTLNEPWVVTDGGYLNGVLAPGHSCHYEAPVAAHHLMRAHGAAVQAYRAVGAHEIG
                     LVVNIEPKYSASDDPKDLAATNRADAYMNRQFLDPALLGSYPEEMKEIFGDAWPEWPA
                     KDFDLICQKIDFVGLNYYTRAVTCDKPDALPVRAGTVPQPHKTYTETGWEVYPDGLSD
                     TLVWLKNRYGEMPLYITENGSAFFDPPKAVHGRVVDPVRMHYLNQHLCAVRTAIGQGV
                     DVRGYMAWSLMDNYEWALGYAKRFGIVHVDFETLERTPKDTARYYSKIIESNGGILDE
                     PFLPVSLHLRHG"
     gene            1982..3106
                     /gene="ugpC"
                     /locus_tag="PKM72_06415"
     CDS             1982..3106
                     /gene="ugpC"
                     /locus_tag="PKM72_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019292901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNP60455.1"
                     /translation="MASLSLRNIHKSFGKTAVLSGIDLDVADGEFTVIVGPSGCGKST
                     LLRTVAGLEEIDKGTIKIGEIDVSRLPPSERGIAMVFQSYALYPHMTVYANMSFGMKI
                     AGASPEEIDQAVRAAAETLGLTALLERKPKELSGGQRQRTAIGRAIVRRPRIFLFDEP
                     LSNLDASLRVKMRHEIARLHETLETTMIYVTHDQVEAMTLADRIVVLREGCIEQTGSP
                     HELYKYPVNRFVAGFIGSPAMNFLSGFVVESRPDITTVRLESGAMIQVNGVAGALPPD
                     TPVTLGIRPEHLTTGGDHNLIETQIKLVEPLGSHSYVYLETPDAEDQLIFRASGDFSF
                     SKGDRLTVCIAADLCHLFGKDGRELRHKPSGNLTTSQGDH"
     gene            3106..6225
                     /locus_tag="PKM72_06420"
     CDS             3106..6225
                     /locus_tag="PKM72_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNP60456.1"
                     /translation="MTFFKRSLLTCVSILVLLVLPLRAALATSNIPPKSTWRVTASSI
                     QSDEFLPGYLADGDMATRWSSQAKDEEWALIDLGEVVEITGFTLHWENGFSSKYNLLI
                     STDAKVWTTVYENNDSDGQMDDIYIQPIFGRYVRLDTRERATAWGNSLWEFDIKGMDD
                     LVDISVVAGGGGDVRVMMDGRKDTVWKSDAVDRAELLLDLRKSRSITGLRIDWGELYA
                     GSVDMAISLDGKAWNQVSSISVSEGSQGQSTFVPHHVTEVRYIRLVLADPIKAGGGFG
                     VGDISLRGPKEAFTPFIQYQFDARKAPFGDYPPHLRGYQTYWTLVGLPVDTEESLFDE
                     YGNLEFQADAPMLMPFIREGENLYSAAGAKKITQHLQDGYLPVPIVQWETGNGLHFRS
                     EAITSGPVDASITYVRHTLENRSDTERKGEIIVTVRPAQINPEWQHGGLSPINSIRYK
                     HETGKPVIINGKDSYLSLSPADSFLAPQYAHGDIGNALRGRVVLDARADKPGDTVSIG
                     DETRTGLLSAAWTYAFELKPGESKEVVLAAPLHDTLEHMKTAADFGKLREEYVTFWTD
                     KLDRVGFSLAAESVVNTVKSQVAYILLNNDGVVIQPGARSYNRTWMRDGAIISSTLMR
                     LGFFEEVRGFLNWYAERVEPDGLVPPILFTSGEVYDGWGRNIEWDSQGQFIFAIMEYY
                     RFTGDRDFLEKHFEAVHRAMKYMVILRERTLDPKHYEHLEAGERLKGILPPSISHEGF
                     ITPMHSYWDDFWAIRGWADGIEMAEILGRKFLADWAKEEAKNFSTSVRESIEKTIAWK
                     SLDYIPVDADKGTPDPTSIAIAMFPTEAWKVLPADILDRTFREYYQMVKKRDASSLPY
                     SYTPYEVRNILALATLGYRKEAFHLHEILFKGRRPANWNEFAEVVLSDSRKGVYIGDM
                     PHTWVGAGYVNSVRGLIVMEEDNSKTLNLLFGTPREWLVGDGITLSNFPTHFGNLMMQ
                     AKWDDNDQKLSLDVDVQKDIARKIYVRWPIHKEGKPEQVTVEGDANYRIDDHGIWLSG
                     NKFKLVAKW"
     gene            6605..9106
                     /locus_tag="PKM72_06425"
     CDS             6605..9106
                     /locus_tag="PKM72_06425"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNP60457.1"
                     /translation="MGMGWRIGKTKNLAIIVMLCVMVVGLQSPNVAGETIGRHAKNMS
                     FPSQCAEEDNVNIPVYGGYVSDYQITATFPTYYPLKRGQKDLCIGDLTNCEQAWDMRP
                     TAQIAYYDAAQQKLKFVWQSPGFYWYPIETPDAVNAEVGQYVSLAYPPTDRYWVGLGS
                     PPAMAYYDAFKQNLMYIVRDENGKWREPEIVDSTGDVGQFVSLRISRDRRHHVVYYDA
                     DHGDLKYTYKSNPDAVGGWAPPTILDATGNVGQHAAMAISPSGHLHVVYYDADKKDLK
                     YVTNDDTHGWTAPVVLDAVGDVGTYASMSVYYEGWTEKMNGAYEGHYPHVTYYDASNG
                     NLKYIYQDKQGWSTPLVLDAAGDVGQYSSTAFGPDGALHVAYYDATNKDLKYLHKESP
                     SAQWPVGIVLDAAGDVGRYASIGVTARGNPNIAYYDADKQSLKFLAKTKTTAPQWDNP
                     QVIASGSAGQYASLVTSGFVDIADLDGKDIYNDGDVAVRVMDVSGWPYESGMAVNIVG
                     GASEPDHDFIQFLKRTDDDHAPPQIFVLYANGYARIITQPPVGREERVCFGSSMIIGP
                     AAEDPLFPFIPIEKLDIDPHTMTIKVRYKNGSSAVLQLQAEPGKTTVRVTDVTYDTLT
                     APFVTMRSMWVANDNSDISRIQAESSSGVVQTYTIDPTHEEWTTIPGDSWSFDRPTYS
                     RHNTSCPDFKIDVLEPTGYFVTRQAEDYRSGTLKKTSRKTASGGQTVQGMGEATYVLN
                     LTEEIANPSIRLRYTKGKGAGDVVNISVDGRIQSKIEMIDEGAIGEYMVTPEMRLSHP
                     LPAGSHTIQLEVQSNTGALELDYFVIHSQTEHPGQ"
     gene            9748..12231
                     /locus_tag="PKM72_06430"
     CDS             9748..12231
                     /locus_tag="PKM72_06430"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNP60458.1"
                     /translation="MKAVIDRKRWWTLAMGSGVAGLLAFAGAGFLGAGVTIPVPVAYA
                     EANLNDGWGNGDQSGSLIGLDPEGKFTQAFPLKHTDVSIQVSGPLARAQVTQQFENPY
                     PDKIEAVYTFPLPQSAAVDAMTLTVGDRVVKGTIKKREEARAIYEAAKRRGQVAGLLD
                     QERPNIFTQSVANILPGEKITVTISYVETLSYEDGVYSVIFPMVVGPRYIPGKPIVQI
                     PSLGEGWAYDTHQVPDASHITPPVLKTGTRSGHDLSLSVRIDAGVPIQQVQSATHDID
                     VNNSQASQAEVHLKREATIPNKDFILTYAVAGNDIHDAVLTHRNGENGFFTLLLQPPD
                     RVKTDEVTPKELVFVLDTSGSMSGFPIEKAKETMRLAIDGLNPRDRFNLITFAGDTHI
                     LFPEPVPATPANILKAQKFLASRSGGGGTEMMKAIRAALEPSDSQEHVRIVCFMTDGY
                     IGNDMAILGEVQKHPNARIFSFGIGSSVNRFLLDRMAEQGRGEVEYVGLNDDGSAAAR
                     RFHERVQHPLLTDLSVDWEGLDVAEVYPKRLPDLFSAKPLVLTGRYTTPGKGTIRLRG
                     KVAGRSVVRNIPVDLPGFAPEHDVLPSLWARTKIAELMAQDFDGVQQGNPRKDVREAI
                     TQLGLDFRLMTQFTSFVAVEELIITEGGQPRRVEVPVELPEGVSYEGIFGSGDEHAGS
                     FRQHLMQSPATMSQSFLGGKVAREYVASSPPAMVDRVEPERDDSRRGDSSNKMNSTLE
                     SLVAHVEKATVLPQDVAQHVTDGKVLLQVWLTDTNPETLEKLKELDFEVVAQPKTGTL
                     VIGRLSVEKLDVFSKLSVVQYITLQRAGT"
     gene            12889..13440
                     /locus_tag="PKM72_06435"
     CDS             12889..13440
                     /locus_tag="PKM72_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60459.1"
                     /translation="MSRIEFGKREWRLFCWNLNLCLAAIFIGLLAIIVRYLSLEMLEN
                     LKGYYGGLVLIKTFLDNYSLDILFYGGFSYLLAPYCLVLPATAVDLKPSLAWSSEQTN
                     GNELRLALLFGGLPFVFGFLYCPPSLLTWLESDQMLIVKHVFRPFLVYLVTPIIVIVL
                     SIAFRELTNWTPSSSHSEKASLA"
     gene            complement(13498..13884)
                     /locus_tag="PKM72_06440"
     CDS             complement(13498..13884)
                     /locus_tag="PKM72_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNP60460.1"
                     /translation="MKVLLDTCTFLWIISDDKTLSPESREIFSDPSHEILLSAISVWE
                     ILVKYSLGKIPLPTPVDQFILQQRERHGISTLALEEAATGHLPKLPALHKDPFDRMLI
                     CQTIQHELTILTPDPLITQYAVRTAW"
     gene            complement(13881..14126)
                     /locus_tag="PKM72_06445"
     CDS             complement(13881..14126)
                     /locus_tag="PKM72_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNP60461.1"
                     /translation="MIKLNIHEAKTHLSRYLDKLKNGETILLCKHNKPVAEIRGLPAY
                     PSRKRPIGLAKGQFTIPDSFFEPLPNDLLKAFSGLSE"
     gene            14443..14865
                     /locus_tag="PKM72_06450"
     CDS             14443..14865
                     /locus_tag="PKM72_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60462.1"
                     /translation="MDTLQLNPSPKPLKVLPIIREAFSFAWDQRRALWGWIVVGAFLS
                     GLTDLVDPFLKIEEEGEVDAWPTVLMVMQYCSILFLASTPRTLVFVMLAIYCHRSILI
                     DHGGNCPNLRLIFDRGIGGFLPGFLWCILEHFYVSFQE"
     gene            14826..15320
                     /locus_tag="PKM72_06455"
     CDS             14826..15320
                     /locus_tag="PKM72_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60463.1"
                     /translation="MYTGAFLCIFPGISLIGIAALAGFFSLEIDNVYSNTVWMKTLVE
                     YLFLYGVFLSPFLYILGRWSLVFPAIAIDHRPSLSWSWKQTKHNGWRMAVLVGFLLLI
                     VGNLNHVLPFIGLSEFPMFSSYISSFVWFIFTPVEVAVISFAFRELTNWNSSAPLFSH
                     PGEA"
     gene            complement(15458..16381)
                     /locus_tag="PKM72_06460"
     CDS             complement(15458..16381)
                     /locus_tag="PKM72_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP60464.1"
                     /translation="MSTLAIFVIPFVVILGIVLASLTLPFWRRDPSPVSFGLDDDRAQ
                     ELLDLQIERETVVRSLQDLEMELSQGRMDPTDYERLKATDERRLLNLLDRLDTFKDVV
                     HDETSEQHSAASKRINWIPTIASGLVVLLASVGIYGALQFKAAQKLMAIEAEMGGGPN
                     PMEMVAQLEKRLKENPDDLQGQIMAGRSYQALNRIPEARKAWEKVLELDPKQHEAHYN
                     LGVIQIETRQIDDPEIFKQALTHFDIVLADLPNQPAVNWYRGLALWYLNRRQETDAAW
                     TLAAQNMEPGSKDMEFVKDALIKLRAGEVPF"
     gene            complement(16398..16862)
                     /locus_tag="PKM72_06465"
     CDS             complement(16398..16862)
                     /locus_tag="PKM72_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNP60465.1"
                     /translation="MPIRLTWCLVALLTIPLPLFAAVQDETDVDIQVREIAKTLRCTV
                     CQTENIWESGAPLAQQMRGVIRDRIALGHNTEEIRAYFLSRYGDYILMEPPKHGVNWL
                     IWLAPFLLLVVGGFFLYKEVTHWVRDTPTPPSQPEPPLDDQARKRIERELES"
     gene            complement(16862..>17052)
                     /locus_tag="PKM72_06470"
     CDS             complement(16862..>17052)
                     /locus_tag="PKM72_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60466.1"
                     /translation="DLKGQLAIDYGVYGVPETFVIDQQGKIRHKWVGPITGEVYTNIT
                     QKVIAPLLKAQPTTTTTS"
BASE COUNT         3931 a         3938 c         4788 g         4395 t
ORIGIN      
        1 atacctgggt gggatcggat ttcatccggg cggtgcttga tctgtttgtc tatgccgatc
       61 cagaaaccgg ctctctggtc atcggtgccg gtctgccgcg tcattggcta cagggtgaag
      121 ggattcgggt gcgtggattg aaaaccccgt atggcgaggt ctcgtatacg gcgcatgatg
      181 aggatgggca ggtactcgtt gatttggaag gatcctccat gccacccggt ggctttgttt
      241 ttccagcttc cctgaaggga gacctcaagg taacattcaa tggagaccct gtgaatgtgc
      301 atgcaacatc cccataaaac cggttgtcgg gaattggatt gcgtgccagc atttcggttg
      361 taagtccctc tcctttgtaa ggagagggta ggtgaggtta gattccgggg gctatcagtc
      421 aatcagtacc ggagcaacca gtaaaggctt cgcagaggct acatcctctt tgtctttgtt
      481 cctcttctga acaaagcctt acaacggagg agaattccag gccggtgcct gccgaacaag
      541 gagcacgagg aggggattga cctgtgaaaa aattagataa agaaaaaagg ataagggtgg
      601 atgaacaaac aaacgacatt tcccgagggg tttctatggg gtgcggccac atcggcctac
      661 cagattgagg gggcgccgct ggcagatggg gcaggtccga gcatctggca gcgattttca
      721 catacaccgg gacataccct gaacggcgag accggtgacg tcgcgtgcga ccattaccat
      781 cgctggcgag aggatatcgc cctcatgaag gaactggggt tgcaggccta tcggttttcg
      841 gtggcctggg ggcgtatttt tccggaaggc aaggggcggc tgaatgagaa aggtctggat
      901 ttctacgaca aactggttga tggccttttg gaagcaggga ttgaaccgct cctgacattg
      961 tatcactggg atctgcctga ggcgttggac gatctcggcg gatggttgaa tccggacatt
     1021 gcgcattggt ttgccgacta tggggaagcc atgtaccgac ggctggatgg ccgggtcaaa
     1081 aaatggacga cactcaatga accctgggtc gtcacggatg gcggctattt aaatggtgtc
     1141 ctggcccccg gacattcctg tcactatgaa gcgcccgttg ccgcgcatca tctgatgcgt
     1201 gcccatggtg ccgcggttca ggcctatcgc gctgtcggtg ctcatgaaat cggcctcgtg
     1261 gtgaatatcg agcccaagta ttccgcttca gatgatccca aagatctcgc agccaccaat
     1321 cgcgcggatg cgtatatgaa ccggcagttt cttgatccgg cacttctggg atcctatccg
     1381 gaggaaatga aggaaatatt cggtgacgcc tggcctgaat ggccggccaa ggatttcgac
     1441 ctgatttgtc agaagatcga ttttgtcgga ctgaattatt acacccgggc cgtgacctgt
     1501 gataagccgg atgccttgcc ggtgagggcc gggaccgttc cccaacccca taagacgtat
     1561 acggaaaccg ggtgggaggt ctatcccgac ggactttccg atacgttggt ctggctcaag
     1621 aatcgttatg gtgagatgcc gctgtatatc accgagaatg gctcggcatt ctttgatccg
     1681 ccgaaagcgg tgcatgggcg ggtcgttgat ccggtacgga tgcattacct gaatcaacat
     1741 ctttgcgctg tccggaccgc gattgggcag ggcgtggatg tgcgcggata tatggcctgg
     1801 tctctgatgg acaactatga atgggcactt ggttatgcca aacgattcgg cattgtgcat
     1861 gtcgattttg agacgcttga acgaacgccg aaggataccg ccaggtatta ttcgaaaatt
     1921 attgaatcca atggaggaat tctggacgaa ccctttcttc cggtttcttt gcatctgcgt
     1981 catggctagt ctgtccctac gaaatattca caagagtttt ggcaagaccg ccgtgttgtc
     2041 gggcattgac cttgatgttg ccgatgggga gtttacggtg attgtcggcc cttcgggttg
     2101 cggcaagtca accctgttgc gtaccgtcgc gggccttgag gagatcgata aaggaaccat
     2161 taagatcggt gagatcgatg tctcgaggct tccgccttcc gagcgcggta ttgcaatggt
     2221 gttccagtcc tatgcgctgt atccgcatat gaccgtctat gccaatatga gcttcgggat
     2281 gaaaattgcc ggagcgtctc cggaagaaat cgaccaggcg gtgcgcgcgg ctgccgaaac
     2341 gctcggtctg acggcgctgc ttgagcggaa accgaaagaa ctttccggtg gacagcgtca
     2401 gcgcacggct attggccggg cgatcgtcag gcgtccgaga atcttcctct ttgatgaacc
     2461 gctttccaat ctggatgcat cccttcgtgt gaagatgcgt catgagattg cccgactgca
     2521 cgagacgctt gaaaccacca tgatctatgt cacgcatgac caggttgagg ccatgacatt
     2581 ggcggatcgc atcgtggtgc tcagggaagg atgtattgaa cagacgggtt ccccgcatga
     2641 attgtataag tatcctgtta accgctttgt ggcaggcttt atcggatcgc ctgccatgaa
     2701 ttttctgtcc gggttcgtgg tagaaagccg cccggatatc accacggtcc gacttgaaag
     2761 cggagcgatg attcaggtga atggcgttgc cggagcatta ccccctgata ctccggtcac
     2821 gcttggcatt cgtccggagc atctgacgac ggggggtgat cacaacctca tcgagacgca
     2881 gatcaagctg gtggagccac tcggcagcca ttcctatgtc tatctggaaa cgccggatgc
     2941 cgaggaccag ctgatattcc gcgcatccgg cgattttagt ttttcgaagg gagataggct
     3001 gacggtgtgt attgcggcag acctatgcca tcttttcgga aaagacggta gggaacttcg
     3061 gcacaaacct tccggaaacc tcacgacaag tcagggagat cactgatgac gtttttcaaa
     3121 agaagtctgt tgacgtgtgt gtctattctg gtcctgctgg tattgcccct gagagcggcc
     3181 ctggcaacat cgaatatacc gccaaagagc acctggcggg tgactgcttc ttcgattcag
     3241 agcgatgaat tccttccggg gtatttggca gatggtgaca tggcgacccg ctggtccagt
     3301 caggctaagg atgaggaatg ggctctcatt gatcttggag aggtcgtgga gatcacaggc
     3361 tttaccctgc attgggaaaa cggattctcg agcaaatata accttctgat ttcgaccgat
     3421 gccaaggtgt ggacgacggt ctatgaaaat aacgacagtg acgggcagat ggatgatatt
     3481 tatatccaac ccattttcgg gagatatgtc cggctggata ccagggagag agcaaccgct
     3541 tgggggaatt ctctctggga atttgatatt aaggggatgg atgaccttgt agatatatcc
     3601 gtggttgccg gaggcggggg cgatgtcagg gtcatgatgg atggtcgcaa ggataccgtc
     3661 tggaaaagcg acgcggtcga ccgggctgaa ttgctcttgg atctgcgcaa gtctagaagt
     3721 attaccgggt tgcgcattga ctggggcgag ctctatgccg gttcggtgga catggcgatt
     3781 tccctggatg ggaaggcatg gaatcaagtc tcctccatct ctgtttccga gggttcacag
     3841 gggcaatcca cttttgtgcc gcatcatgtc accgaggtcc gctatatcag gcttgtgctg
     3901 gctgatccca ttaaagccgg gggcggtttc ggcgttgggg atatttcttt gcgtggtccc
     3961 aaggaagcct tcaccccttt tattcagtat caatttgatg cccgaaaggc tcccttcggg
     4021 gattatcccc cccatctcag gggataccag acctactgga ccctggttgg tcttccagtc
     4081 gataccgagg aaagtctgtt tgatgagtat ggcaatcttg aattccaggc cgatgccccc
     4141 atgttgatgc cctttatacg agagggtgaa aatctgtatt cggctgccgg agctaagaaa
     4201 ataacccagc acttgcagga cgggtacctc ccggttccca tcgtgcaatg ggagacggga
     4261 aacggacttc atttcagatc cgaggctatt acgagcggcc ctgtcgatgc cagcatcacc
     4321 tatgtgcgac atacccttga aaatcgctcc gatactgaac ggaaggggga aatcatcgtc
     4381 acggttaggc cagcccagat taatcccgag tggcaacatg gcggactgtc gccgattaat
     4441 tcgatccggt acaagcacga aaccgggaaa ccggtgatta tcaacggcaa ggacagctac
     4501 ctctccctct cgcctgccga cagtttcctc gccccgcaat atgctcacgg agatattggc
     4561 aacgctctgc gaggcagggt cgtcctggac gcaagggcag ataagccagg cgataccgtc
     4621 tctattggtg acgagaccag aaccggtctc ctctcggcag cctggacgta tgcgtttgaa
     4681 ttgaaacccg gcgaatccaa ggaggtggtg cttgcggccc ccctgcatga cacccttgaa
     4741 catatgaaaa cggccgctga cttcggaaag ttgcgcgagg agtatgtaac tttctggacc
     4801 gacaagcttg accgggtggg attttcgctt gccgcagaat cggtcgtgaa tacggtgaag
     4861 tcgcaggtag cctatatcct gttgaacaat gatggcgtgg tcattcagcc cggtgcccgc
     4921 agctataacc ggacctggat gcgggatggc gctattattt cctccaccct catgcgactt
     4981 ggttttttcg aggaagtcag gggttttctg aactggtatg ccgaacgtgt ggaacctgat
     5041 ggcctcgtgc ctcccattct cttcaccagc ggtgaggtgt atgacggctg gggcagaaat
     5101 atcgaatggg acagccaggg gcagttcatc tttgcgatca tggagtatta tcgctttacg
     5161 ggtgacaggg attttctgga aaagcacttt gaggctgttc accgggcgat gaagtacatg
     5221 gtcatcttgc gggagcgtac gcttgatccg aaacattatg aacatcttga ggccggtgaa
     5281 cgtctgaaag gcattctccc cccgtccatc agccatgaag gtttcattac cccgatgcac
     5341 agttattggg atgatttctg ggctatacgc ggttgggcgg acggtattga aatggcggag
     5401 attctgggcc ggaaattttt agccgattgg gcgaaggagg aggcgaaaaa tttcagtaca
     5461 agtgtcaggg agtccattga aaagaccatt gcctggaagt ctctcgacta tatccctgtg
     5521 gatgcggaca aaggcactcc tgatccgacc tccattgcca ttgccatgtt tcccactgaa
     5581 gcctggaagg ttctgcccgc agatatcctg gaccggacat tccgggaata ttatcaaatg
     5641 gtgaaaaaac gggatgccag ttctctaccc tattcgtaca cgccatacga ggtgcgcaat
     5701 attctggctc ttgcaactct tgggtatcgc aaggaagctt tccacttgca tgaaattctc
     5761 tttaagggca ggcgtccggc caattggaat gagtttgccg aggtcgtcct ctccgacagt
     5821 cggaagggtg tgtatattgg tgatatgcca catacgtggg tcggggcggg atatgtcaat
     5881 tctgttcgtg gactcatcgt gatggaggag gacaacagta aaaccttgaa cctgctgttt
     5941 ggtacgccac gtgaatggct ggtgggcgat gggattactc tttcgaattt tcccacacat
     6001 ttcggaaacc tgatgatgca ggcaaagtgg gacgacaacg atcagaagct gagtctggat
     6061 gtggatgtgc aaaaagacat cgccaggaag atttatgttc gctggccaat ccataaagag
     6121 ggtaagcctg agcaggtgac cgttgagggc gatgccaact accgcattga tgatcacggt
     6181 atctggttgt caggcaataa attcaagttg gtggccaaat ggtaaatggc cggtttgata
     6241 aggagtaaag agtaagggtc aggggtggga tgaatgactg tagcctatgc ccctgtcatg
     6301 aaggtattca cctgattttt tgcggagtct ttgccatggc ttaggatctc ctttttcagg
     6361 accctccttt gtcggttgtc gttcacccgc taccggggtt catgttcctg gacgtgtcat
     6421 aaattttgga agaaccgtgc cgccgcatgc ctatggggag tgttgaataa tcctaacttc
     6481 gatgggcata ttgactcaca ggcacgctgc atatcaacaa cctcgggtaa gcaatgcacc
     6541 cgtctgtcga aacccgggca acatgacaat tcaccaatct actgaaacgt aaggagatcg
     6601 cttgatgggt atgggttggc gtatagggaa gacaaagaat ctggcgatca tcgtgatgct
     6661 ttgtgtgatg gtggtgggct tgcaaagccc caatgttgcg ggtgagacca ttgggcggca
     6721 cgccaagaat atgagttttc cgtcgcagtg cgcggaagaa gacaatgtga atattccggt
     6781 ctatggcggc tatgtgtccg actatcaaat caccgcgacc tttcctacct actatccact
     6841 caaacgtggt cagaaggatc tctgtattgg ggatctgacg aactgtgaac aggcgtggga
     6901 catgaggccg acggcgcaaa tcgcctacta cgatgcggcg caacaaaagc tgaaatttgt
     6961 ctggcagtcg cctggcttct attggtatcc gattgaaacc ccggacgcgg tgaatgcgga
     7021 agtgggtcaa tatgtgtccc tggcgtatcc gccgacagat cgctattggg taggcttagg
     7081 ctcgccaccg gccatggcgt attatgacgc gttcaaacaa aatctcatgt acattgtccg
     7141 ggatgaaaat gggaagtggc gggagcctga gattgtcgat tccaccggcg atgtggggca
     7201 gttcgtctcg ctacgcatta gccgggatag gcggcatcat gtggtgtatt acgatgcgga
     7261 tcacggggat ttaaaatata cctacaagag caatccggat gcggtgggcg gatgggcacc
     7321 cccgacaatc ctggatgcca ccggcaatgt cgggcagcat gccgccatgg cgatcagtcc
     7381 atcgggccac ctgcatgtgg tgtactacga tgcggacaag aaggatttga aatacgtcac
     7441 caacgatgat acgcatggat ggacggcccc cgtggtcctg gatgccgtcg gggatgttgg
     7501 aacctacgcc tcaatgagcg tgtattatga gggttggacg gagaaaatga acggggcgta
     7561 tgaaggtcat tatccgcatg ttacgtatta cgatgcgagc aacgggaatt taaaatatat
     7621 ctatcaggat aaacaggggt ggtcgacccc gctggtgtta gatgcggcgg gagatgtcgg
     7681 tcagtattcg tcgacggcgt ttggtccgga tggggctttg catgtggcct attatgatgc
     7741 cacgaataag gatctaaaat accttcataa agaatctccc tcggcacaat ggccggtggg
     7801 gatcgttctt gatgcggcgg gggatgtcgg ccggtatgcc tcgattgggg tgacggcaag
     7861 agggaatccg aatattgcct attacgatgc cgacaagcag agtttgaaat ttctggcaaa
     7921 aacgaaaacg accgcaccgc agtgggacaa tcctcaggtc atcgcatcag gttccgccgg
     7981 gcaatatgcg agtctggtga cttcgggttt tgtggacatt gccgatctcg acggtaagga
     8041 catctacaat gacggcgatg tggcggttcg agtgatggat gtatccgggt ggccctatga
     8101 atcgggtatg gctgttaata tcgtgggagg ggcatcggag ccggaccacg atttcatcca
     8161 gttcctcaaa cgaacggacg atgatcatgc tccccctcaa atatttgtgc tctatgccaa
     8221 tggatacgca cggattatca cgcaaccccc ggtgggacgg gaggagcggg tctgtttcgg
     8281 atcgtcgatg atcattgggc cggcggcaga ggatcccctg tttccgttca ttccgatcga
     8341 aaagttggat attgatcccc acacgatgac catcaaggtg cggtataaaa acggatcgtc
     8401 tgcggtgctg cagcttcagg ccgaaccggg caagacgacg gtacgggtga cggatgtgac
     8461 gtacgatacc ctcaccgcgc cgtttgtcac aatgcggtcc atgtgggtgg ccaatgataa
     8521 tagcgatatc tcccgcatcc aggctgaatc atcgtccggt gtcgtgcaga cgtatacgat
     8581 cgatccgacg catgaggaat ggaccactat cccgggagat tcctggagtt ttgaccgccc
     8641 cacctattcg cggcataata cctcctgccc ggatttcaag atcgatgtgc tggaaccaac
     8701 gggctatttc gtgacccggc aggcggagga ctatcgctcg gggaccttga agaaaacgtc
     8761 ccgcaagacg gcatccgggg ggcaaacggt acaagggatg ggggaagcga cctatgtgtt
     8821 gaacctgacc gaggagattg cgaatccgag cattcgtttg cgatatacca agggcaaagg
     8881 cgctggtgat gtagtcaata tctctgttga tggccgaatc cagtcgaaga tcgaaatgat
     8941 tgatgagggt gccatcggtg aatatatggt gacgccagag atgcgattat cacatccact
     9001 tcctgccggt tcccatacga tacaattgga agtgcaatcg aataccggcg cattggaatt
     9061 ggactatttt gtcatccaca gccagaccga acatcctggt caatagcagt cagatgcttc
     9121 aagatatttc ctccaccgct gtttgtgccc cggtaccggt gtgcatcggt gaagattgac
     9181 cttgcccatg acgcgatgcc atttcctatg accagtcaac ctgaatgtat tttgtgtgtt
     9241 ggcagttggt ggaactttaa ggagctggtg cggaaattta tattttttat attttctcca
     9301 cgcgatttgt ccgatgaaag ttggatcata ccgaaattgt agctcaaacc cttcgacgtt
     9361 actttcaggc caagctctag aaggctccgt gcctctgctt gtcatcctca cccgtttatg
     9421 gaccccatca gaattcattt gttcagaatg atgagcgttg ttgggggcat gtcaggacgg
     9481 ttgcatattg cagtcggttt gaataggcca accgccttca ctcctgatcg ttttgtctcc
     9541 actgattatt atcgtttcta gaattccggc ccgccccaca aagatgccac gtgaatgtag
     9601 gccgatatgc cgttggcata ttattattca acatttttta ttctattctg ttttattctt
     9661 ttagggattt ctctgaaagg attctcaaag tcctgccata ccctcctgtg aagcggcatg
     9721 gggtcgctga aaataaggag ggaacacatg aaagcggtaa ttgacagaaa acgatggtgg
     9781 acgttggcga tgggaagtgg agtggccggg cttctggctt ttgctggagc gggttttttg
     9841 ggtgcaggag tcaccattcc tgtcccggta gcctatgctg aggcaaatct caatgatgga
     9901 tggggcaatg gagatcaatc cggaagcctg atagggctgg atccggaagg aaaattcacc
     9961 caagcctttc cccttaaaca cacggatgtt tccattcagg tcagcggccc cctggcgcgg
    10021 gctcaggtaa cccagcaatt cgaaaatcct tatcccgaca agattgaagc cgtctatacc
    10081 tttccacttc cacaatcggc ggcggtggat gcgatgacgc ttacggttgg agaccgggtg
    10141 gtgaaaggga cgattaaaaa gcgagaagag gcgagagcta tttatgaagc agcgaaacgg
    10201 cgaggccaag tggcggggct tttggatcag gaacggccga atatcttcac gcagtcggtc
    10261 gcgaatattc ttccggggga gaagattacg gtgacgatta gttacgtgga gacactctcc
    10321 tatgaggatg gagtctattc ggtgatattt ccgatggtgg tggggccgcg ctatatcccg
    10381 gggaaaccca tagtccagat cccatcgctc ggcgagggct gggcctatga cactcatcaa
    10441 gtccctgatg cctcacatat cacgccgcct gttttgaaaa cgggcacgcg atcaggacat
    10501 gatctgtcgt tgtccgtcag gatcgatgcc ggtgtcccga ttcaacaggt ccaatctgca
    10561 acccatgata ttgatgtgaa caattcacaa gcttctcagg cggaagtcca cctcaaacgg
    10621 gaagcgacta ttcccaacaa agattttatc ctgacctatg ctgtggcggg gaatgatatt
    10681 cacgatgcgg tcctgacgca ccgaaatggg gaaaatggtt ttttcacgtt gctgctccag
    10741 ccgcctgatc gggtgaagac tgatgaagtc acacccaagg aattggtctt cgtgttggat
    10801 acctccgggt ccatgtcggg ttttcccatc gaaaaagcaa aggaaaccat gcgcctggcc
    10861 atcgacggat tgaacccccg cgataggttt aatctcatca cgtttgcggg tgatacccat
    10921 attctgtttc ccgaaccggt tcccgcaacc ccggcgaata tcctgaaggc ccagaagttt
    10981 ctggcctcac ggtcgggcgg cggtggaacg gaaatgatga aggccattcg tgcggcgcta
    11041 gaaccctccg attcgcaaga acatgttcgc atcgtgtgtt tcatgacgga tgggtatatc
    11101 ggcaacgata tggccattct gggcgaagtg cagaaacatc ctaacgcccg aatcttttcg
    11161 ttcgggattg gtagcagcgt caaccggttt ttattggaca ggatggccga acaaggccgt
    11221 ggagaggtgg aatatgtggg tttgaatgat gacggctcgg cagcagcccg gcgctttcat
    11281 gaacgggttc aacatccgtt gctcacggat ctgtccgtgg actgggaagg gctggatgtg
    11341 gcggaggtct atccaaagcg actccctgat ttgtttagcg cgaaaccgtt ggtgttgacc
    11401 ggtcggtata cgacaccagg caagggcacg atacggttgc gcggcaaggt ggcagggcgt
    11461 tcagtggtgc ggaatattcc cgtcgatcta cccggttttg ctccagagca tgatgtcttg
    11521 ccttctttgt gggcgcgaac caaaattgcg gagctgatgg cgcaggactt cgatggtgtt
    11581 cagcaaggaa acccccggaa ggatgtacgg gaagccatca cgcagttggg cttggatttt
    11641 cggctgatga cccagttcac ctcatttgtc gcggtggaag agttgattat cacggaaggt
    11701 ggacagccac gacgtgtaga agtaccggtg gaactgccgg aaggggtaag ctacgaaggc
    11761 atttttggaa gcggtgacga acacgcagga agttttcggc aacacctgat gcaatcgcca
    11821 gcgacaatga gtcaatcttt tctgggagga aaggttgccc gggaatacgt ggcctccagt
    11881 cccccggcta tggtggatcg tgtggagcct gaaagggatg attctcgtcg tggggactca
    11941 tcgaacaaga tgaattctac actcgaatcg ttggtggccc atgtggagaa ggcaaccgtg
    12001 ttgcctcagg acgtagcaca acatgtgacc gatgggaagg tattgcttca agtctggttg
    12061 acggatacca atcctgagac cttggagaag ttgaaagaac tggactttga agtggtagct
    12121 cagcccaaga cgggcacgct tgtgatcggg aggctgtctg ttgaaaaatt agatgtcttc
    12181 tcaaaattgt ccgtggtgca gtatatcacg ttgcaacgtg caggaacgta aaaccgaggt
    12241 ggttagaatc aggcggctcg ggtatttcat ccgagccgct tttttttgcg ttattcctgg
    12301 atgcctttag caggaatcca tcttttctac cctgctgagt gagtaaaatg gacgcccgcc
    12361 acaaaaggcg ataggcctga aagacgatgt tcttaaagtt tcctgaaaat ttcacaaccg
    12421 gcttggatca aagcgcattc tcattgtggg cattacgtca tttttctact caataaaggc
    12481 ggttgatgtc cccaggtctg agtgcgaagg acactttgcc gataagtgaa agcataatag
    12541 tgagcgtttg agtgcgtttt tcgtgtgata gctatgaccc aattatctca gagcattcct
    12601 ctgaaaaaac ttcccgtcct ggcgacgatt cgagaaaccc tcatggtttc atggcagcat
    12661 cggattagat tgttccgctg gattattgga tgcaccctta tcgccgggac cctttgttat
    12721 ctccaagagt accttgtcgt aaagtacgtt tgggccaata aggactggaa tcctttatca
    12781 ggtagaattt tcctttttat tttatctgca tttcccaccc taatgatttc tgcaatgttt
    12841 tcggtttttt gccatcgttt agttttagca aacggggagg ggaactcctt gagtcggatt
    12901 gaatttggta aacgcgagtg gcgattattc tgctggaatt taaatctatg tttggccgca
    12961 attttcattg gccttcttgc aataatagtc cgttaccttt ctctggaaat gttggagaat
    13021 ttaaaagggt attatggggg cctggtgttg ataaaaacat tcttggataa ctattcattg
    13081 gatattttgt tctatggagg attttcctat cttcttgcac cctattgtct ggttcttcca
    13141 gcaactgcag ttgatcttaa gccatctttg gcatggtcat ccgaacaaac taatgggaat
    13201 gaattgaggc tggcactatt atttggaggc ctgccgtttg ttttcgggtt tttgtactgc
    13261 cctccctcgc tattgacttg gttggaaagt gatcaaatgt tgattgtgaa gcatgtgttc
    13321 aggccatttc ttgtgtattt ggttactccg atcatcgtaa tcgtgttatc catcgcgttt
    13381 cgtgaactta ccaattggac tccatcttcc tcccattccg aaaaagcctc tttagcttaa
    13441 agcccctttg cgcaattttt tgtcctgttg ccagtaacac attggtttca aaggcatcta
    13501 ccaggcggtt cggacggcat attgggtaat gagtgggtca ggagtgagga tggtcagttc
    13561 atgttgaata gtttggcaaa tgagcatgcg gtcaaaaggg tctttatgaa gagcaggaag
    13621 tttgggcagg tgtccggttg cggcttcttc caacgcgagt gtgctgatgc catgccgctc
    13681 ccgctgttga aggatgaatt gatccactgg tgtggggagg ggaatcttac ccagagagta
    13741 tttcacgaga atttcccaga cggaaatcgc actcaacaaa atttcatgcg aggggtcgga
    13801 aaatatctcc cgactttctg gcgatagggt cttgtcgtct gaaatgatcc acagaaatgt
    13861 acaggtatcc agcagaactt tcactcagat agtccggaaa atgccttgag taggtcattg
    13921 gggagaggtt caaaaaagct atcgggaatg gtgaactgac ctttggctaa gccaataggt
    13981 cgtttgcggg atgggtaagc aggtaaccct ctaatttccg ccaccggttt attgtgctta
    14041 cacagaagaa tcgtttctcc atttttaagt ttatccaaat agcgggataa atgggttttg
    14101 gcttcgtgaa tattaagttt aatcatagtc caaatataaa ctaaaaatta aactaaggca
    14161 aggtctgtgg caatttggag gcttagccat gggttttcgg ttcaaccagg tacaattgag
    14221 actttacctg tggctatttc tgctcgcagc ccttggtaat accaccaaag attcagagtt
    14281 tttggctttc aaagggagaa atgggtatgc atcgtttttt ttgaaatgaa attctccccc
    14341 tccactataa agtagagcct aaactcctct cctgcggcca ttccaattaa ccgatattaa
    14401 ttggaaaatc catcgcctca aattacctgt gttttggcct ttatggacac tctgcaactt
    14461 aacccttctc ctaaaccact caaagttttg cccataattc gcgaagcttt tagttttgcg
    14521 tgggatcagc gtcgcgcact ttggggttgg atcgttgttg gtgcattcct ttccggttta
    14581 acagatttag tcgatccatt tttaaaaata gaggaagaag gggaggttga tgcctggcct
    14641 actgttctga tggtgatgca atattgttcg atattatttt tggcgtcgac accaaggact
    14701 ttggtttttg tgatgttagc aatttattgt catcgttcaa ttcttattga tcacgggggg
    14761 aattgtccca atcttcgatt gatttttgat cgagggattg gaggtttttt gcctggattt
    14821 ttgtggtgta tactggagca tttttatgta tctttccagg aataagcctc atagggatag
    14881 cggccctggc agggttcttc tcattggaaa ttgataatgt ctattcgaat actgtttgga
    14941 tgaaaactct agttgaatat ttgtttttgt atggagtttt tctcagccct ttcctctaca
    15001 ttcttggtcg ctggagttta gtgtttcctg ccattgccat tgatcatcga ccaagtttaa
    15061 gttggtcatg gaagcaaacg aaacataatg gttggcgcat ggcagtttta gttggctttc
    15121 tcctactgat tgtagggaat cttaatcatg ttttgccttt catcgggctc tctgaattcc
    15181 ctatgttcag ctcctacatt agttcctttg tctggtttat atttactcct gttgaagttg
    15241 cagtgatctc gtttgccttc agagaactca ctaattggaa ttcttctgct ccgctatttt
    15301 cgcatccagg ggaagcgtga attttctctg ctcttatggg aaaaggtgct gagtgcacac
    15361 tttaaaaaaa aggatactct tgaagcgagg gttgggtgtc cacttgtggt gtgcaacgga
    15421 caagattttt ggaagtgcga ggaaccgaaa agaataatta aaacggaact tcaccggcgc
    15481 ggagtttaat gagggcgtct ttgacgaact ccatgtcttt gctgccgggc tccatgtttt
    15541 gtgcggcgag cgtccaggcg gcgtcggttt cctggcggcg attcaaatac cacaaggcta
    15601 aacctcgata ccagttgacg gcaggttgat tgggaagatc cgccaggaca atgtcgaaat
    15661 gagtgagggc ctgtttgaaa atttccggat cgtcaatttg acgcgtttcg atttggataa
    15721 cgcctaaatt gtagtgggct tcatgctgct tcgggtccag ctccaagact ttttcccagg
    15781 ccttcctcgc ctcagggata cgattcaagg cctgatagga tcgcccggcc atgatttggc
    15841 cttgcaggtc atcaggattc tctttgaggc gtttctccag ttgggcgacc atctccatgg
    15901 gattcggccc tcctcccatt tccgcttcaa tggccatcag tttttgtgcg gctttgaact
    15961 gaagcgcccc gtaaatgccg acggaggcca ataagaccac caggcctgag gcgatggtcg
    16021 gaatccagtt tattcgtttc gaggcggcag aatgctgttc gctggtttcg tcgtgaacga
    16081 cgtccttgaa ggtgtccagc cggtctaata gattgagtag acggcgttcg tcggtggctt
    16141 tcaaccgttc gtagtcggtc gggtccatcc ttccctgtga taattccatt tcgaggtcct
    16201 gtagagaacg gaccaccgtt tcccgttcga tttgaaggtc aaggagttcc tgagcccggt
    16261 catcgtccag gccaaaggag accggtgaag gatcccgccg ccagaatgga agagtaaggg
    16321 aggcaagcac gatccccagg atgaccacaa acggaatcac gaaaatggcg agagtcgaca
    16381 tacgggattg agactattca ggactcaagt tcacgctcga tacgttttcg agcctgatcg
    16441 tccaatggcg gttcaggttg gctgggtgga gtgggcgtat cacggaccca atgagtgact
    16501 tccttgtaaa gaaagaatcc cccgacgacg agcaggagaa acggcgccag ccagatgagc
    16561 caattgacgc catgttttgg gggttccatg aggatatagt ccccgtagcg gctgaggaaa
    16621 taggcgcgaa tctcttccgt attgtgtccc agggcgatgc gatcacgaat cactccacgc
    16681 atttgttggg ccagcggtgc gccggattcc caaatatttt ccgtttggca gacggtgcag
    16741 cggagggttt tggcaatttc cctgacctga atgtcgacgt cggtttcgtc ctgcacggca
    16801 gcgaataacg gaagaggaat ggtcaatagc gccacaaggc accaggtaag tcgaatgggc
    16861 attaggacgt cgtggttgtg gtgggttgag ccttgagcaa cggggcaatg actttttgtg
    16921 tgatgttggt atagacctcc ccggtgatcg ggccgaccca tttatggcgg attttcccct
    16981 gttgatcaat cacaaatgtt tcgggtaccc catagacccc gtaatcaatg gccaattgtc
    17041 ctttgaggtc ac
//
LOCUS       DAORAQ010000049        16814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_593_length_16814_cov_60.547228, whole genome shotgun sequence.
ACCESSION   DAORAQ010000049 DAORAQ010000000
VERSION     DAORAQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_593_length_16814_cov_60.547228"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PKM72_06475"
     CDS             <1..1019
                     /locus_tag="PKM72_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNP60467.1"
                     /translation="AHGLATPLGINSTTGVAGLTLGGGFGWLSRKYGMTIDNLLSAHV
                     VTADGRQVHASATEHEDLFWGLRGGGGNLGIVTSFEFQLHPVGPDVLSGLIVFPFDQA
                     KSVLTQFARFTETIPDELAVWMVTRKAPPLPFLPAEVHGKEIVALALCYAGDPTLGRT
                     LIEPLRRFGTAHGEHIGVQPYMAWQQAFDPLLSRGARNYWKSHNFSKLSDGAIDAVIQ
                     YVGTLPSPQCEIFVAAIGGQTARVAPEAMAYSSRDANYVMNVHARWEGAADDEACIAW
                     AREFFAASQPFASGGAYINFLTQDETDRIAFAYGATYNRLVELKKKYDPTNLFRMNQN
                     VRPV"
     gene            1367..2752
                     /locus_tag="PKM72_06480"
     CDS             1367..2752
                     /locus_tag="PKM72_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNP60468.1"
                     /translation="MKALQSRIAGKVTVATDAGYEDLRCSLIWNQLTPGRYPKVVVQV
                     ANEQDVIEAVRFARAQNLKVSVRGGGHSWVGFSLCDGSLLIDLGRLNQVSIDPMSRTA
                     VVQPAVTGRELNRQLSAHELAFPVGHCPSVPMSGFLLNGGLGWNSNTWGPACFSVEAA
                     HVVTADGTLVVVNQEQHADLLWAIRGAGPGFFGVLTQFVLKLYPVPRAMTTSTYFYPL
                     SHMEEVGAWAEGMARQLPREVELAMFCAPAPPAYIEQCRSGNGFVCLLTATAFLDSQR
                     EAAARLNLLEACPVLSACLGKEMIQPTSLDALLDKGGMLWPDYHRALAETFWSNSSPV
                     RPLATMRESFLHAPSSKSLGACIFATGSESSAAMLPAAAFSMTARTLLLCYAIWERPE
                     EDDANRAWHRETIAALNRFAVGHYVGESDIIEELVRAERSFAPANWQRLQSLRHTYDP
                     DRLFHTHFGTG"
     gene            3066..3386
                     /gene="sugE"
                     /locus_tag="PKM72_06485"
     CDS             3066..3386
                     /gene="sugE"
                     /locus_tag="PKM72_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HNP60469.1"
                     /translation="MNWAILVIAGLFEVAWAIGLKYTEGFTRLWPTVGTVLAMVISLG
                     LLGIAMKTLPVGTAYAVWVGVGAVGTAILGIFLFEEQANPARLVSLALILAGIVGLKL
                     ATHA"
     gene            3467..3838
                     /locus_tag="PKM72_06490"
     CDS             3467..3838
                     /locus_tag="PKM72_06490"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNP60470.1"
                     /translation="MSEYVEFLKEVFEEFGPIHPRRMFGGYGLFHKGLMFGLVADDVL
                     YLKADETLSPFFEERELEPFQYKKHGKTFTMSYYMAPEEIFEDTAEAKGWALRSYEAA
                     VRAKKPVKKVKQKKSRSRFNL"
     gene            3901..4494
                     /locus_tag="PKM72_06495"
     CDS             3901..4494
                     /locus_tag="PKM72_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNP60471.1"
                     /translation="MTRTSNDIPGDTLRGEMIGTVYIATSVDGFIARENGGIDWLPGV
                     GDAGTEDYGYHEFIDSVDAIVMGRASYEMALSFGAWLYGEKPVVVLSSRPVAIGKDIA
                     RTVESMDAPPHEVVQRLAGRGWRHLYIDGGKTIQGFLREDLIHRLIITKVPILLGTGI
                     PLFGSLSHEIHLEHLDTRSFENGLVQSHYEVRRNIAG"
     gene            4620..4838
                     /locus_tag="PKM72_06500"
     CDS             4620..4838
                     /locus_tag="PKM72_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNP60472.1"
                     /translation="MPYVNIQMTTGASREQKAELVKDVTDSLVRVLGKNPDYIHVVIQ
                     EINEEDWGFSGLLTDEWKKQQDRSSSSE"
     gene            4799..5725
                     /locus_tag="PKM72_06505"
     CDS             4799..5725
                     /locus_tag="PKM72_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP60473.1"
                     /translation="MEKATGPIVLKRMTIDHLGAVGPMNLSLRHGDPSDAEPCGTICY
                     EAFKSIAEQHNFPPDFPAPDIAVQLFSHLFSRPDLHSVVAEVEGRIVGSNFLWENAII
                     AGVGPITVDPAVQNGAVGRRLMEHVLERAQEIRFASVRLVQAAYHNRSLSLYTKLGFQ
                     AREPLSTIQGPALGLSVSGHAVRQAVEKDLAGCNRLCLQVHGHERESELLDAIKQQTA
                     TVVERDGRISGYATLVGFFGHAVAENNEDLKAIIGAATGFPGPGFLLPTRNSDLLRWC
                     LENGLRVVQPMTLMSMGLYHEPDGAFLPSVLY"
     gene            5770..6582
                     /locus_tag="PKM72_06510"
     CDS             5770..6582
                     /locus_tag="PKM72_06510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP60474.1"
                     /translation="MNSNKALWEKGDFTRIANTMRESGETLVQRLGITNGLKILDLGC
                     GDGTTALPAAKLGAQVLGVDIASNLVQAGNRRAREHGFVNCSFQEGDASDLHQLPDQA
                     FDLVVSIFGAMFASNPFAVAKEQVRVTRPGGRIVMGNWIPNDPTLVAQILKISSSYMP
                     PPPEGLVSPMTWGVESHVVERFAGAGIPAEKISFTRDTFTFKYPGAPSVFVDEFKKYY
                     GPTMNAFEAAEKNGRGADLQKELEDLFNNQNTSLHAGSTLIPATFLRVTIAL"
     gene            6745..6903
                     /locus_tag="PKM72_06515"
     CDS             6745..6903
                     /locus_tag="PKM72_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60475.1"
                     /translation="MNEISAFVLEYGGNVLFSIVSGKQIGLPIPAIPVMLAAGALACM
                     AWNGVCPE"
     gene            7024..7521
                     /locus_tag="PKM72_06520"
     CDS             7024..7521
                     /locus_tag="PKM72_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HNP60476.1"
                     /translation="MVSRIVSGGQTGADRAALDVALHLGIDCGGWVPNGRMAEDGVIP
                     ARYPNLVETKFDDPSIRTEYNVRDSDATLLITRGAPAGGSAFTVEVAMRLGKPVLHVD
                     LLRESVEQIVPRVCQWLQDLHPVILNVAGPRASEDREIYNFTTVVLKCVFSALKSRMN
                     IGPEL"
     gene            complement(7570..9231)
                     /locus_tag="PKM72_06525"
     CDS             complement(7570..9231)
                     /locus_tag="PKM72_06525"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNP60477.1"
                     /translation="MGEFQYQEIFEHQADTTSYRKLTSDYVSTTNCEGQELVKVQPEA
                     LTLLAREAMDDIAHLLRPSHLAQLAKILEDPEASENDRFVAIELLKNANIASARVLPG
                     CQDTGTAIAMGYKGQQVFTTGDDAEALSRGIFEAYDTRNLRYSQMAPLDMYTEKNTGT
                     NLPAQIDLYAKPGSEYHFLFIAKGGGSANKTFLYQETKALLNPKSLLAFVAEKIRTLG
                     TSACPPYHLAFVVGGLSAEFTLKTVKLASCHYLDDLPTSGNNQGRAFRDLDLERQILQ
                     LCAETGIGAQFGGKYFAHDVRVIRLPRHGASCPVGLGVSCSADRQILGKITKEGIFLE
                     QLETNPAKYLPEVTDEDLNGHVIKINLNRPMADILTELRKHPVATRLSLTGPIVVARD
                     IAHARIKEQLDAGKGLPQYLKDHVVYYAGPAKKPQGHASGSFGPTTAGRMDSYVDQFQ
                     EAGGSMVMLAKGNRSKQVREACGKHGGFYLGSIGGPAARLAEHSIKKVEVLEFEDLGM
                     EAVWKIEVEDFPAFIVINHEGKDFYADLTGQRSSGLIQVGNSAEG"
     gene            9592..10800
                     /gene="glgA"
                     /locus_tag="PKM72_06530"
     CDS             9592..10800
                     /gene="glgA"
                     /locus_tag="PKM72_06530"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNP60478.1"
                     /translation="MKALMFTNEFPPYTYGGAGAHVEYLSRELSHLLSVEVRCFGDQN
                     LEQGNLTVHGIPLGETPFAAPKPLHSVIGAVQRCWQMNADGMGADLVHCHTWYTHFGG
                     IMAKLNYGIPLVITTHSLEPLRPWKREQLAGGYDFSLWVEKTALEMADAIIAVSEGTK
                     KDILRLFQVPEERIHIIHNGIDLQEFRKVQSEEVLIRYGIPTGQPYVLFVGRMTRQKG
                     ITYLIQALSYLDEEFPVVLCASSPDTPAMAAEMKEALDQISAHRKHIYWIPDILDKPS
                     LIELYSHAGVFCCPSIYEPFGIINLEAMACEVPVVASAVGGIPEVVVEDKTGFLVPVD
                     QFEEAPFSPKDPDRFAQDLADRLNQLMRDPELRVPMGQAGRRRAEELFGWDKIAKRTK
                     ALYETVLEGS"
     gene            complement(10927..11160)
                     /locus_tag="PKM72_06535"
     CDS             complement(10927..11160)
                     /locus_tag="PKM72_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60479.1"
                     /translation="MHAEDELLESLRSFNDCEIRVYTRFATEWRDQRLTDGSEAEVSF
                     WNSMISMLVEERHRRKEEVQRLEAMFQTGRDPG"
     gene            11292..11552
                     /locus_tag="PKM72_06540"
     CDS             11292..11552
                     /locus_tag="PKM72_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60480.1"
                     /translation="MINKLSSVQVCYRWIGLGVFCFVIWGCGEDPRQLFETAQFEEQQ
                     HNRSHAQELYEQIIHQHPESEFAEKARQRIEAWKTENPEEGK"
     gene            complement(11567..11833)
                     /locus_tag="PKM72_06545"
     CDS             complement(11567..11833)
                     /locus_tag="PKM72_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60481.1"
                     /translation="MMSHHHEPIPHTEDLPNFLREFDDSDLQRYTCFSSEFRDQRMEA
                     GSMAEAGFWNTIVNLCIDERMRRDQDIRRLEYMYRTGVDPEHHS"
     gene            complement(12060..12755)
                     /locus_tag="PKM72_06550"
     CDS             complement(12060..12755)
                     /locus_tag="PKM72_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNP60482.1"
                     /translation="MIWVIIPTYNEEKALPQTIESVYSQSPPYRVIVVDGGSTDRTLE
                     ILRQHSQFSCFTAPKGRASQMNAGARFVTQQQANAEDWLLFLHADTQLPRGAFRHLRH
                     LSSDSAYQAGGFRHHFSGKDWRLRMISWLDNLRCTHSHIVYGDQGLFVRQGLFTRLGG
                     FPTQTVLEDVRFGQTLLTQTTPRLLPLTVITDSRKFVKMGVWRSFIRVLMIILYVEFG
                     LPTFSPAFFRDIR"
     gene            complement(12778..13461)
                     /locus_tag="PKM72_06555"
     CDS             complement(12778..13461)
                     /locus_tag="PKM72_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60483.1"
                     /translation="MLVKPGEHVHLAQLLQFMVLGEQLAHDCAKVQATLVREPGMQRF
                     LIGQAKQEGRHALVFQWAIRWLAPRGPHSLLISHQMNQYRRLLTSALTRGDVAESLLA
                     EQIILEGLGQAILQKLETGLVKRDAPFRKFRRILLQQEEAHHGFGLRTLNRMLDDGQT
                     SIDYLRERAPAYLDLGKALLFSAQESFHFIREDPREYWNDFQQGLPMWLREHPQDHPL
                     VPNGLHAGE"
     gene            complement(13920..14735)
                     /locus_tag="PKM72_06560"
     CDS             complement(13920..14735)
                     /locus_tag="PKM72_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNP60484.1"
                     /translation="MEWITRISIPMRWCIILNISFVVSSGCSTIPNTFTPSHPISPET
                     FTHETFHQAVLAHVKNGAVDYPQLAHDSNFKRYLNLLQQIAPQQLPTPNHRLAFWINV
                     YNAFAIKGILNGDSPATLTGRYTFFIGRTYVVGEESLNLYDLEQGLLIPDFKEPRIHF
                     AIVCASQSCPKLQSAAYTPEDLDQQLTASAQQFINDPTRNRFDRQRQIAYLSRIFDWF
                     SEDFINHSGSLLGYVAQFVSDQDLANEIRQNSYTIEFLEYDWSLNGVPPLSPQ"
     assembly_gap    15082..15181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15182..16717
                     /locus_tag="PKM72_06565"
     CDS             <15182..16717
                     /locus_tag="PKM72_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60485.1"
                     /translation="HHFPISSNGAMAQRYFDQGLILSFGFNHAEAARSFRESQRRDPN
                     CAMCYWGEALVLGPNINAPMDPSVVPQAFAAVEKALTLKDQATEKEGALIQALAMRYS
                     KEVMADRSPLDLAYAEAMRVVAQQFPDDPAIGALLAEALMDLHPWDFWTRGGEPQPWT
                     PEIVSRLETVLDQSASHPLANHLYIHIMEASPHPEKALPSAERLPALVPGSGHLVHMP
                     AHIYIRVGRYRDAVLANQHAVKVDEYYLNHAHEESLYTAAYVPHNSHFLWAAAIKLGQ
                     QKLAMQAALDTAASVKSEMMRAPGIAGTMQHFWTIPLYTQIAFGQWTNILAEPKPPVD
                     LRYPTAIWHYARGLAFVRQGKLEAAQQESMKLAEIARDPAVAKLTILDLNNIVDILAI
                     AEAVLAGEMAAGHGEYETAVRQLQRAVKLEDGLNYTEPKDWYLSSRQVLGAVLLEAGN
                     AGEAERTFREDLQDHPQNGWVLFGLEQSLRAQGKTDEAGSALQSFRQVWSDADVTLTA
                     ARF"
BASE COUNT         3733 a         4026 c         4828 g         4127 t
ORIGIN      
        1 aggcccatgg cctcgccacg ccgctgggca tcaactcgac cacgggcgtg gccggcctga
       61 cgctcggagg cggcttcggc tggctgagcc ggaagtacgg tatgaccatc gacaacctgc
      121 tttccgcgca tgtcgtgacg gcggacggcc gtcaagtgca tgccagcgca acggaacacg
      181 aagacctctt ctgggggctg cgtggtggtg gtgggaattt gggtattgtc accagctttg
      241 agtttcaact ccatcccgtc gggccggacg tcctgagcgg gctcattgtc ttccccttcg
      301 accaggcgaa gtcggtgttg acgcagttcg cccgtttcac cgagacgata ccggacgagc
      361 ttgccgtctg gatggtcacc cggaaggcac ccccgctgcc ctttctgccg gcggaggtcc
      421 acgggaaaga gatcgtcgcc ctcgcattgt gctatgcggg cgatcccact ctgggcagga
      481 cgctgattga gccgctgcgc aggttcggca cggcccacgg tgagcacatc ggcgtgcagc
      541 cctacatggc ctggcaacag gccttcgatc cgctcttgtc gcggggtgcg cgcaattact
      601 ggaagtcgca taacttttca aaactgagtg acggcgcgat cgacgccgtt attcaatatg
      661 tcggcacgct tccgtcgccg caatgcgaga tcttcgtcgc cgcgatcggc ggccagacgg
      721 cccgcgtcgc ccccgaggcg atggcctact caagccggga cgccaactat gtgatgaacg
      781 tgcatgcccg ttgggaggga gctgccgatg acgaggcctg catcgcctgg gcgcgcgaat
      841 tctttgccgc gtcacagcca tttgcgagcg gtggtgcgta catcaacttc ctcacccaag
      901 acgagaccga tcgcattgcg ttcgcctacg gtgcgacgta caaccggttg gtggagctta
      961 agaagaagta cgacccgacg aacctcttcc ggatgaacca gaacgtcagg ccggtctgag
     1021 aggaggtgag gggcctcata ctcgcatgca gcagagggcg cgtcggatcc ctgctgatgc
     1081 aggtcgttat gcctagacaa caggtcagga gcaatcctag gtccgaatac attgaatatc
     1141 tccaagaggt atgtgcgcca ttgggccgat ttggacacgc cggctgttcg gtgacgatgg
     1201 tccctttcgc aatggtgtga ggttgggtct ggttgcggat gattgataaa accagaggtc
     1261 accaaaacaa caaaaaggac ccatcagttc cccattctta cgatgaagca catcattcct
     1321 cctgctgcct gtggagcttc gcagagggta gaatattagg atggacgtga aggctctgca
     1381 gagtcggatt gcgggcaagg tgacggttgc gaccgacgcc gggtacgaag atctcaggtg
     1441 ctcgctcatt tggaatcagc tgacgccggg gcgttacccg aaggtggtcg tgcaggtggc
     1501 caatgagcag gatgtcatcg aggctgtcag gtttgctcgc gctcagaatt tgaaagtttc
     1561 cgtgcgtggt ggtgggcata gttgggtcgg gttttcgttg tgcgacggga gcctgttgat
     1621 agatctcggg cgtctgaacc aggtttcgat tgaccctatg agtcgcacgg cagtggttca
     1681 accggcggtg acggggcggg agttaaaccg gcagttgtcc gcgcatgagt tggcctttcc
     1741 cgtgggacat tgcccttcgg tccccatgag cggattttta ctgaatggcg gtttgggctg
     1801 gaactctaat acatggggac cggcctgctt tagcgtcgag gcggcccatg ttgtgacggc
     1861 cgatggcacc ttagtcgtgg ttaatcagga gcaacatgcc gacttactct gggcaattcg
     1921 cggggcgggg cctggctttt tcggggtgtt gacccaattc gttctcaagc tgtatccggt
     1981 gccacgtgcc atgacgacca gcacttactt ctacccgttg tcacacatgg aagaggttgg
     2041 cgcatgggca gagggaatgg ccaggcagtt gccgcgagag gtggaattgg cgatgttctg
     2101 cgctccggca ccaccggcct atatcgaaca gtgccggtcc ggcaacggct ttgtctgtct
     2161 cctgactgcg acggcctttc tcgattccca acgtgaagcc gctgccaggc tcaacctcct
     2221 ggaggcctgt cccgttttat ccgcatgtct gggaaaggaa atgatccaac cgacttcgct
     2281 ggacgccttg ctcgataagg gcgggatgtt atggccggac taccatcgcg ctctggcgga
     2341 aacgttttgg tcgaactcct cacctgtacg gccgttggcg acgatgcgcg agtccttttt
     2401 gcacgcgccg tcatcaaagt cactgggggc atgcatattt gccaccggtt cggagagtag
     2461 tgctgcaatg ctccccgccg ccgcattctc gatgacagcc aggacgttac ttctctgcta
     2521 tgcgatatgg gaacggcctg aggaggatga tgcgaacagg gcatggcatc gtgagacaat
     2581 tgcggcattg aaccggtttg cggtgggtca ttacgtgggc gagtccgaca ttatcgagga
     2641 actggtacgc gccgaacggt cgttcgcgcc tgcgaactgg caacgcctcc agtcgctgcg
     2701 ccatacctac gaccctgata gactttttca tacccacttc gggacgggct gactataaga
     2761 gatctttcgt actatccttc cctgccactc agtggaggca cagttcagtc ccaattctat
     2821 gagtgagagc gttgaaattc tcaaagaggg attggaacaa ttcagaccca tccgtccctg
     2881 tcggatgtac ataatgcttg agtgaccatt ttttgctata gatacatcca tgtctcatct
     2941 gtcgtgccag ggggccgcct tcggtggccg ttgaactcca gcgtgaggcc ccactgagaa
     3001 gtgaggatga cctcactgtg cgacagcata tcgggacaac cccacatttt tgagagggac
     3061 ttgttatgaa ctgggcgatt cttgttattg ccggattatt cgaagtcgca tgggcaattg
     3121 ggctcaaata cacggagggc tttacgcgtc tgtggccgac tgtcgggact gtgctggcca
     3181 tggtaatcag ccttggcttg ctcggtattg caatgaaaac acttcccgtc gggacggctt
     3241 atgcggtttg ggtcggtgtc ggtgccgttg gcactgccat attgggaatc ttcttgtttg
     3301 aggagcaggc gaatcccgcg cgactggtta gtctggcatt gatccttgca ggcatcgttg
     3361 gcctcaagct cgcaacccat gcctaactct tcgttccagt agacgggttt ctggggccgc
     3421 tgaattccaa cctttgtcgt aaaaaagcac cccggacaat aaatttatga gcgaatacgt
     3481 ggaatttctc aaagaggtgt tcgaggaatt cggacccatt catccacgcc gcatgtttgg
     3541 cgggtatggg cttttccata aggggttgat gttcggtctg gtggcggatg atgtgttgta
     3601 tttgaaagct gacgagacat tgtccccatt ttttgaagaa cgggaattag agccattcca
     3661 gtataaaaaa cacggcaaga cattcaccat gtcctactat atggctcccg aggagatctt
     3721 tgaggatacg gcggaggcga agggctgggc gcttcggtcg tatgaggcgg ccgttcgtgc
     3781 aaaaaagccg gtgaagaagg tcaagcaaaa aaaaagccgg tcacggttta atctttagtt
     3841 ccaggaaatt tttcctaaaa gtgcattcgc tgatagcccg cgcaggagga aaaattcagg
     3901 atgacgcgga catcaaacga tattccagga gacacattga ggggcgagat gatcgggacg
     3961 gtctatattg ccaccagcgt tgatggtttt atcgcccgcg agaatggcgg tatcgactgg
     4021 cttcccgggg tgggagatgc cgggacggag gattacgggt atcatgaatt cattgattct
     4081 gttgatgcca ttgttatggg acgggcctca tatgagatgg cattatcctt cggggcatgg
     4141 ctctatgggg aaaagcccgt ggtggtgctc agcagtcgtc cggtggcgat tggcaaggac
     4201 atcgcaagaa cagtggagtc catggatgca ccaccgcatg aggtggtgca acgcctggcc
     4261 ggacgtggat ggaggcatct ctatattgac ggaggcaaga ccattcaagg attccttcgc
     4321 gaagacttga ttcaccgact gatcattacc aaggttccaa ttcttctcgg cacgggcatt
     4381 cccttgttcg ggtcgctttc ccacgagatc cacttggaac atcttgacac acgatcgttt
     4441 gaaaacggtt tggtgcaaag ccactatgag gtgcgacgga atatcgccgg gtagaagact
     4501 tcttcctggc gtgattgcac ccggccactc ggcccatggg cggtgaattt tttttgtagt
     4561 gtggcaaggc gtgtcacgtg acatccaatc ctctgactca agtatactaa ggccgtctta
     4621 tgccatacgt gaatattcaa atgacgacgg gtgcctcaag agagcagaag gcggaattgg
     4681 tgaaagacgt cactgattcc ctggttcgcg tgttaggaaa aaacccggat tacatccatg
     4741 tcgtgattca agagattaac gaggaagact ggggattttc cggacttctg actgatgaat
     4801 ggaaaaagca acaggaccga tcgtcctcaa gcgaatgacg attgaccatt tgggggcagt
     4861 gggacctatg aacctcagtc tgcgccatgg tgacccttcc gatgctgaac cgtgtggcac
     4921 gatctgttac gaagcgttta agtccatagc cgaacaacat aattttcctc ctgattttcc
     4981 tgccccggat attgccgttc agttattttc tcacctgttc tcgcgtcctg accttcattc
     5041 agtcgtagct gaagtggaag ggcgaatcgt cgggagtaat ttcctctggg agaatgccat
     5101 tattgccggg gtcggtccga tcaccgtgga cccggcggtc caaaatgggg ccgtgggtag
     5161 acgcctgatg gaacatgtgt tggagcgggc gcaggagatc cgtttcgcga gcgtccggtt
     5221 agtgcaagcg gcctatcaca atcgttcact gtctctctat accaaattag gtttccaagc
     5281 ccgtgaaccg ctttcgacga ttcagggtcc ggcccttggt ctctcggtat ccggccacgc
     5341 tgttcgtcag gcggttgaaa aggatttggc gggttgcaat cggttgtgtc ttcaggtcca
     5401 tgggcacgaa cgggaatccg aactgctgga cgccattaaa caacagacgg ccaccgtggt
     5461 cgaacgcgat ggtcgcattt cggggtatgc cactctggtt ggttttttcg gccatgcggt
     5521 tgccgagaac aatgaagacc tgaaggcgat catcggtgcg gctaccggtt ttcccggtcc
     5581 gggatttttg ctccccacgc gcaatagtga tctgcttcgc tggtgtctgg aaaatgggct
     5641 gcgggtcgtt cagcccatga cgctgatgag tatggggttg tatcacgaac ccgacggggc
     5701 ctttctgcct tccgtgctgt attaagagag gaagggcggc taatcaatcc ttatataagg
     5761 actacttcca tgaattcaaa taaagcgctt tgggaaaaag gtgactttac gcggattgcc
     5821 aacaccatgc gggaaagtgg ggaaacgctt gttcagcgac tcggaatcac aaacgggctc
     5881 aagattttgg atctcgggtg cggcgatggc acaaccgcat tgcctgcggc aaaactcgga
     5941 gctcaggtat tgggtgtcga tatcgcgagc aatttggtcc aggccgggaa taggcgggcc
     6001 agggagcatg gctttgttaa ttgttccttt caggaaggcg atgcgtccga tttgcaccaa
     6061 ttgccggacc aggcgtttga cctcgtcgtg agtatcttcg gggcgatgtt tgcgtccaat
     6121 ccgtttgcgg tggccaagga gcaagtgcgg gtgacccgac ccggtggccg gatcgtgatg
     6181 gggaactgga ttccgaatga tccgacgctg gttgcgcaga ttttgaaaat cagttcaagc
     6241 tacatgccac cgcctcctga gggattagtc agcccgatga cgtggggggt cgaaagccac
     6301 gtggtcgagc gatttgcggg tgcgggaatt ccagccgaga aaatatcttt cacacgggac
     6361 acgtttacct tcaagtatcc aggcgcccct tcagtatttg tggatgaatt caaaaaatac
     6421 tacggaccaa ccatgaatgc cttcgaagcc gccgaaaaga acggccgggg tgcggacctg
     6481 cagaaggaat tggaagattt gttcaacaac caaaacacaa gcctacacgc tggttccaca
     6541 ttgattcctg caaccttttt gcgtgtaacc attgcgcttt gatggggtcg catacgggaa
     6601 ccatgcgggt tcagtgtaat ttgtgagccg aactgtaacg atggcgttgg agtcggtaga
     6661 gtctggaatt gaacaatcga atgctatgac cgcctgtgtg gggtttctcc atgaaatcct
     6721 gagccacatc cggcaggagc caaggtgaac gaaatcagcg catttgtgtt ggagtatggc
     6781 gggaatgtct tgttttccat cgtctcaggt aaacaaatcg gacttccgat tccggctatt
     6841 ccggtaatgc tggcggccgg ggcgctggct tgcatggcct ggaacggggt atgtcccgaa
     6901 tagggatgta tagctacatg agtctattat tgagttcgtt ctatagaata ctccctgcga
     6961 atcgccaggt tttgttgacc ctacccggtc gcggactcta accggctgaa aaattccatt
     7021 gttatggtta gtcgaattgt ctccggcggt caaacgggtg ctgaccgtgc agcccttgat
     7081 gtcgcgcttc atttaggaat tgattgtggc gggtgggttc cgaatgggcg gatggcagaa
     7141 gacggcgtca ttcccgcacg ttacccgaac ctcgtcgaga caaaattcga cgatccgagc
     7201 atccggactg agtataatgt gcgggactca gatgctacgt tgctcatcac ccgtggagca
     7261 cctgccggag ggtcggcatt cacggttgaa gtcgccatgc gtctcggcaa gcctgttctt
     7321 catgtcgatc tccttcgaga atcggtggaa cagatcgtgc ctcgggtatg tcagtggttg
     7381 caggatcttc atccggtcat cctgaatgtg gccgggccta gagccagcga agatcgtgaa
     7441 atttataatt tcactacagt tgtgttgaaa tgtgtctttt ctgcgctgaa aagcaggatg
     7501 aacattgggc ccgaattata atattggagt tatagctctg gagataaaat ctcacctgcg
     7561 tgcatcattt tacccttcag ctgaattacc aacctggatg agaccggagg atcgttggcc
     7621 ggtgaggtcg gcgtaaaaat ccttgccttc gtgattgatg acgatgaagg ccgggaaatc
     7681 ttccacttcg attttccaca cggcttccat accgagatct tcgaattcca ggacttccac
     7741 ttttttgata ctatgttcgg ccaaccgcgc ggccgggcct ccgatggatc ccaggtaaaa
     7801 cccgccatgc tttccacagg cctcccgcac ttgtttggag cgattgcctt ttgccagcat
     7861 caccatgctg ccgcctgctt cttgaaactg gtcgacgtag gaatccatcc gtccggcggt
     7921 ggtggggccg aacgatcccg atgcatggcc ttggggcttt ttagcaggtc cggcgtaata
     7981 gaccacatga tctttcaggt attgtggtaa acctttcccg gcatccagct gttctttaat
     8041 tcgggcatgc gcaatatccc gcgccacaac gatggggccg gtgagggaga gacgtgtcgc
     8101 cacgggatgc ttgcgaagtt ctgtcagaat gtctgccatc ggccggttta agttgatttt
     8161 tatgacatgg ccattcaaat cttcgtctgt cacctcgggt aaatatttcg caggattggt
     8221 ttcaagctgt tccaggaaga tgccttcctt ggtgattttt ccaaggattt gtcgatcggc
     8281 tgaacaggag acgcccaaac ccaccgggca ggaggctccg tggcgtggca agcggatgac
     8341 tcgcacgtcg tgagcgaagt actttccccc gaattgcgca ccgatgccgg tctcggcgca
     8401 gagttgaaga atttgtcttt ccagatccag gtctcgaaag gcacggccct ggttgttccc
     8461 tgaggtgggc agatcatcca ggtaatggca actggccagt tttaccgttt tcaatgtgaa
     8521 ttctgcagag aggccgccca cgacaaaggc cagatgatac ggcggacagg ccgaggtgcc
     8581 gagtgttcga attttttccg ctacgaacgc gagcagggat ttgggattta acagggcttt
     8641 ggtttcttga tagagaaagg ttttattcgc ggaacctccc cctttggcaa tgaataaaaa
     8701 atgatactcc gatcccggct tggcgtagag gtcgatttgt gctggaagat tcgtgccggt
     8761 atttttttcc gtgtacatgt cgagtggggc catttgggaa taccgcaggt tgcgcgtatc
     8821 gtaggcttcg aaaatcccgc gggagagggc ttcggcatca tcgccggtgg tgaagacctg
     8881 ttgcccttta tagcccatgg cgatggccgt tccggtgtcc tggcagcccg gcaacacgcg
     8941 ggcagaggcg atgttggcat ttttgaggag ttctatcgcg acaaaccggt cattttccga
     9001 ggcttcgggg tcttccaata ttttggccaa ttgtgctaga tggcttggtc gcaacaagtg
     9061 ggcgatgtca tccatggcct cgcgagccag gagggtgagg gcctcgggtt ggaccttgac
     9121 gagttcctgg ccttcacagt tcgtggtgga cacatagtcc gacgtgagct ttcgatagct
     9181 ggtcgtatct gcttgatgtt caaatatctc ctggtactga aattctccca tgtctgtcat
     9241 ccccttcttc gtctataact ttaaatatcc tttagtttcc ccacggtaat cgcaggtccg
     9301 tgggcttggc aatatggttc tcgccggatc gtcaattgtg cgtcttcttt tattgaaact
     9361 tcaagggagt ctcgagtttt tttcggccgt agattagaat gcccccacgc cattttgaat
     9421 gggacatttg agggtgtaag tcgttagtcc gccggtcgtg cgttgtttgg acctggtctc
     9481 tcgattattc tcctgacctt ttttcggaga ggaaggtgac ttcctatgga ttttctttta
     9541 atgtagcagg ctgctggaat tcttcatgat cataatggag tgtgattgcc catgaaagcc
     9601 ttgatgttca ccaatgaatt tcccccttac acctatggtg gcgccggggc tcacgtggag
     9661 tatttgagtc gtgaattatc acatcttctg tccgtcgaag tccgctgttt tggcgaccag
     9721 aacctggaac agggcaatct gactgtgcat gggatccctc tgggagagac tccgtttgcg
     9781 gctcccaagc ctctgcattc ggtcattggc gcggtgcagc gctgttggca aatgaatgcc
     9841 gatgggatgg gcgctgatct ggtgcattgc catacctggt atacccattt tggcgggatt
     9901 atggccaagc tcaattacgg cattccgctc gttatcacca cgcattcatt ggagcctctc
     9961 cggccatgga aacgggagca attagccggc gggtatgatt tttctctgtg ggttgagaaa
    10021 acggccctgg agatggctga tgccattatt gccgtctccg agggaacaaa gaaggacatt
    10081 ctcagattgt ttcaggttcc cgaagagcgg atacatatta ttcataacgg aatcgatctg
    10141 caagaatttc ggaaggtcca aagtgaggag gtgttaatcc gatatgggat ccccaccgga
    10201 caaccctatg tcttatttgt gggaaggatg acacggcaga aggggatcac ctatttgatt
    10261 caagctcttt cgtatttaga tgaggagttt ccggtggtgc tatgcgccag ctctcctgat
    10321 acaccggcca tggcggcgga aatgaaggag gccctcgatc agatttcagc ccatcgaaag
    10381 catatttatt ggattccgga tattctcgat aaaccgagtc tcatcgagtt gtattctcat
    10441 gccggggttt tttgttgtcc ttctatttat gaaccattcg ggatcatcaa tttagaggcg
    10501 atggcctgcg aagtgccggt agtggcttct gccgtggggg gcattcctga agtcgttgtg
    10561 gaggataaga ccggattttt agtgccggtt gatcaatttg aagaagctcc ctttagcccg
    10621 aaagatcctg accggtttgc acaggatttg gccgaccggt tgaatcaact gatgcgagat
    10681 cctgaattac gggtgcccat ggggcaagcc ggaagacgac gggcggaaga actctttggc
    10741 tgggacaaaa ttgctaaacg cacaaaggcg ctctacgaaa ccgttcttga gggcagctag
    10801 ggcatggggc tgtgaggtac caatgtggaa gggaatatga agaggggcta tcgtgttgga
    10861 agctaaggaa ccccaggact ccgggcattc ctggtgaagg ttggaggggg tgcgcgtccc
    10921 ggtctcttac ccgggatcgc ggccggtttg aaacatggcc tcaaggcgct ggacttcttc
    10981 tttgcggcgg tgacgttctt ccaccagcat ggagatcatg gaattccaaa acgagacttc
    11041 agcctctgac ccgtcggtta accgttggtc tctccattcc gtcgcaaaac gggtatacac
    11101 tcgaatttcg caatcgttaa aacttctgag cgattcaagc agttcgtctt cagcatgcat
    11161 aagtcaactg gccttcctga agttggagat gtaaatgatg aatgttggtc acagcgagca
    11221 tgaggatgtg tatcggcggt ttattgcggg ttccttagtg tgagccatca tctatggagg
    11281 agaaattctc gatgataaac aagttatcca gcgttcaagt ctgttatcgg tggatcgggc
    11341 ttggtgtctt ctgtttcgtg atttggggtt gcggcgaaga tcctcgccaa ttatttgaaa
    11401 cggctcagtt tgaggagcaa caacataatc gatcccatgc tcaggaattg tacgaacaga
    11461 ttattcatca acatcctgag agtgaatttg ccgaaaaagc ccggcagcga atagaggcct
    11521 ggaagacaga gaatccggaa gagggaaagt gacgtgcttg aactggtcag gagtgatgtt
    11581 caggatctac gccggttcga tacatgtatt ctaatcgtct aatatcctgg tcccgccgca
    11641 tccgctcatc aatgcacagg ttcacgatgg tattccaaaa tccagcctcc gccattgagc
    11701 cggcttccat ccgttgatcc cggaattcgc ttgagaagca ggtgtagcgc tgcaaatcgc
    11761 tgtcatcgaa ttcacgaagg aagtttggta aatcttccgt atgaggaatg ggttcgtggt
    11821 gatggctcat catgcaatct cttatggaaa atatatcggt ggaggccacc tgaattcttg
    11881 aggtttttgg gagaattttc ttaggaaccc gacgtggctt ctgccatcga agcatgccgt
    11941 ccaggggaga atttgttcat tgtgtacatt tcttgagcgt tagtttctgc tcgtagaaga
    12001 ctcttcgtgc ttaccctaaa gttctagcga tccaacagaa tgagacaagc gggcggggtt
    12061 cagcggatat cacggaaaaa agcaggagaa aaagtgggta gcccaaattc tacatacagg
    12121 ataatcatga ggacgcgaat aaaacttcgc catactccca ttttgacaaa ttttcgggaa
    12181 tcggtaatga ccgtcaaagg caaaagtcgg ggagtcgtct gtgtcaggag ggtctgtcca
    12241 aacctgacat cctccagaac tgtttgcgtg ggaaatcctc ccaatcgggt gaacaggcct
    12301 tgtctcacga ataaaccctg atccccatag acgatatggg aatgcgtaca tcgaagatta
    12361 tccaaccagg aaatcattcg taaccgccaa tcctttccgg agaagtgatg gcggaatcct
    12421 ccggcttgat acgcagagtc ggaagagagg tggcgtaaat gccggaaggc gccgcgaggg
    12481 agttgagtat ctgcatggag aaacaggagc cagtcctcgg cgttggcctg ttgctgggtc
    12541 acgaatcggg cgcctgcatt catttgtgaa gcgcgtcctt tgggagccgt gaaacacgaa
    12601 aattgggaat gctggcggag gatttcaagt gtgcggtcgg tacttcctcc atccactacg
    12661 atgacccggt agggtggcga ttgggaatac acggactcga tggtctgcgg taaggccttc
    12721 tcctcgttgt aggtgggaat gatgacccaa atcataggac gttcctgtga gtggggttta
    12781 ttcacccgca tgaagaccat tcggtaccag ggggtgatcc tgagggtgtt ctcgtaacca
    12841 catgggaaga ccctgttgaa agtcgttcca atattcgcgt ggatcctccc ggatgaagtg
    12901 gaaggattcc tgggccgaaa acagcaacgc ttttcccaag tccaaatacg ctggggcccg
    12961 ttccctcaga tagtcaatgg aagtctgccc gtcgtccaac atcctattca atgtccgaag
    13021 gccaaagccg tggtgggcct cttcctgttg gaggagtatc cggcgaaatt ttcgaaatgg
    13081 ggcgtctcgt ttcacgaggc cggtttccag tttttgtaaa atggcttggc ctaacccttc
    13141 caaaataatt tgttcagcca ggagagattc cgcaacgtcc ccccgtgtca gggcagacgt
    13201 gagcagccgt cgatactgat tcatctggtg ggaaatgagg agagaatgag gaccccgtgg
    13261 agccagccat cggatcgccc actgaaagac cagagcatgg cgcccttcct gcttcgcctg
    13321 gccgatgaga aaccgttgca tgcctggctc cctgaccaag gtggcttgga ctttcgcgca
    13381 atcgtgggcc aattgttcgc ccaggaccat aaattggagt aattgcgcca agtggacatg
    13441 ttcgccaggt ttcaccagca tatcaatatg ttccccaaat tcttgagctc tgcaacgctc
    13501 agttgggcat gagatctctg ttcagaggct tctcatatcc gaaagaaaag atacattgtt
    13561 tctgtacctc aggaaaggct tcttcactag gcagtctccg agggctcttg gatgttacag
    13621 tctacaaaca aaatttcatg aggggagtgt cgaataataa taatttccaa gggcatattg
    13681 agccataggc acgtggcata tcagaggcct cggggcggtt caaaaaagtt cgtccagcaa
    13741 ggccgctaat tgcctgccgg gtcccgaagg gcacaacttt tattttgcgc gcagagcgtc
    13801 cctccagtac gtgagcacgg aaaaatgacg agaacgccgc tgacggcttt tttcaacagc
    13861 cctctgagga aagttggggg ccaaaaaagg agcaagaaag gaatggagcg gcagaattgt
    13921 tattgggggg agagaggtgg gactccattc aagctccagt catattcgag aaattcaatg
    13981 gtgtacgagt tctggcggat ttcgttcgcc aaatcctgat ccgacacgaa ttgtgcgaca
    14041 taccccagta gtgaacctga atgattgatg aaatcctccg aaaaccaatc gaagatcctg
    14101 gaaagatagg cgatttggcg ttgtcgatca aaccggttgc gtgtgggatc gttaataaac
    14161 tgttgagcgc ttgctgtgag ttgttgatca aggtcctcgg gggtgtaggc tgccgattga
    14221 agttttggac aggattgtga ggcgcagaca atcgcaaaat gaattctggg ctctttgaag
    14281 tcgggaatca agagaccctg ttccaggtca tacaaattca gggattcctc acccaccacg
    14341 tatgtgcggc caataaaaaa tgtgtaccgt cccgtcaggg tggctggaga atctccatta
    14401 agaatgccct tgatggcaaa ggcattatag acgttaatcc aaaaggctag acgatggttg
    14461 ggagtcggga gttgctgggg tgcgatttgt tgaaggaggt taaggtaacg tttaaagttt
    14521 gaatcgtgag cgagttgggg atagtccaca gcgccgttct tgacatgggc gaggactgct
    14581 tgatgaaagg tttcatgagt aaaagtctcc ggagaaatag ggtgagaggg agtgaacgta
    14641 ttgggaatag tcgagcagcc cgaggaaacg acaaaggaaa tgttaagaat gatgcaccac
    14701 ctcatcggga tagagattcg tgtgatccat tccatgatgt tggtagggtt caattttgag
    14761 atagctgaaa cgatcacttc ttgtatgctg gatgccccat ccggaaaaaa gtgatgctgg
    14821 aattggaatg ggggtggggg tatctgctcg tgatgggcat cacactcata atggctgtcg
    14881 tcatgttgtg ggtctttcga tggaggcatt ggactgaaga ttaaagaaag agatgggcta
    14941 aggttacagc cggacggttt gtgaaaacac atgaatgacg attactccgg cgatgatcaa
    15001 tcccattcca atgattgctg gcgtatcagg aatttctcga aaggcgactg cacccacgat
    15061 cgccagaaga atgatgccga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 ncatcatttt ccgatctcct ccaatggagc gatggcgcag cgatatttcg atcaaggact
    15241 catcctgtca tttggcttta atcatgccga agcagcaagg tcctttaggg aatcacagag
    15301 gcgagatccg aactgcgcca tgtgctattg gggtgaggca ttggtgctcg gtccgaatat
    15361 caatgccccg atggatcctt cggtggttcc tcaggccttt gcggcggtgg agaaggcctt
    15421 aacgctgaaa gaccaggcca ccgaaaaaga aggcgcgctc attcaggcat tggctatgcg
    15481 ctactctaaa gaggtgatgg ccgatagatc gccacttgac ctggcctatg ccgaggccat
    15541 gcgggtggtt gctcagcagt ttcccgatga cccggccatt ggcgctttgt tagctgaagc
    15601 actgatggat ctgcatccct gggatttttg gaccagggga ggagaacctc aaccctggac
    15661 accggaaatt gtgtcgaggt tggagaccgt attagaccaa tcggctagtc atcctttggc
    15721 caatcatctc tacattcata tcatggaagc ctcgccgcat cccgaaaagg ctttacccag
    15781 tgccgaacga ttacccgccc ttgtcccagg ctcaggacat ctggtgcata tgcccgcgca
    15841 catctatatt cgtgtcgggc ggtatcggga tgccgtgttg gccaatcaac atgcggttaa
    15901 agttgatgag tactatttga accatgctca tgaggaaagt ctatatacgg cggcctatgt
    15961 tccccacaat tcacatttcc tctgggcggc ggcgataaag ctcgggcaac agaagttggc
    16021 gatgcaggcg gcgttggata ccgcggcgtc tgtcaagtct gagatgatgc gcgctcccgg
    16081 catcgcgggc accatgcaac atttttggac gattcctctc tatacccaaa tcgcattcgg
    16141 gcaatggaca aacattctcg cggagcccaa gcctccggtg gatttacggt atcccacggc
    16201 tatctggcat tatgcccgtg gattagcatt tgtgcgtcaa ggaaaattgg aagctgccca
    16261 acaggaatct atgaagctcg cggagattgc ccgtgaccct gcggtggcca agctgaccat
    16321 tcttgatctg aataatatcg tcgatattct tgccattgcg gaggctgttc tggcaggaga
    16381 aatggcggcc ggtcatgggg aatatgagac cgcagttcgt cagttgcagc gcgcggttaa
    16441 acttgaagat ggattgaatt atacggaacc gaaggattgg tatctttcgt cacgccaggt
    16501 gttgggtgcg gtgcttctgg aggctggaaa cgcgggcgaa gcagagcgca cttttcgaga
    16561 agatctccag gatcatcctc aaaacgggtg ggtgcttttc ggattggaac agagtttgcg
    16621 cgcccaggga aaaaccgatg aggctggaag tgcccttcaa tcgtttcgcc aggtatggag
    16681 cgatgcggat gtgaccttga cggctgccag gttttaagct ccctcgtttt tggagcaatg
    16741 ctggaattga ttggaccgtg tgtcattttc cgggcatcag tcattggtgt tttcagtctg
    16801 tgtcccatct cctg
//
LOCUS       DAORAQ010000050        16626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_599_length_16626_cov_67.231066, whole genome shotgun sequence.
ACCESSION   DAORAQ010000050 DAORAQ010000000
VERSION     DAORAQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16626
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_599_length_16626_cov_67.231066"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1588)
                     /locus_tag="PKM72_06570"
     CDS             complement(128..1588)
                     /locus_tag="PKM72_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNP60486.1"
                     /translation="MESEQSVDLTSDTRPDLREEFRFSPLLTVLCLLVCGAGMGLLFW
                     AVTLSDDVRLASPESRDLERIASRMLGFESRLPELSLLEQVMYRLGGQDGETQEQIQR
                     WYEERVNEQSRPLDGLYLGMIYGEAGLTNQLSRIVENWESERNPYALFREFLEVGYGQ
                     SETSSPDYPFLQARLAEEVPTSWFYFHLARRIAVQAGDRNLQANLQLQEYQLTDPPLW
                     KWRALLVGEVVVIGIGVVLLLRLGIARLRGRASPIQTGFIKRGIPWTFREGIAVLARG
                     GALTILLMGLVAVIPDGIGIIEDFGIALLYLPPVVLTAILLCRTRKQSLLQVVGCSNV
                     WQRLKDCLPLVVMLVTFGLVGDWLIVLGGDAFQSSVHWTEWFVPQLIWGTRVELIKTT
                     IDFVLLAPFFEELIFRGILYTTLRTKFSFPLSMVASGLIFALAHGYGLIAFLTVFWSG
                     LLWAWAYERTGSVIPGMVAHAVNNGVVVYSLVSFFR"
     gene            2094..3572
                     /locus_tag="PKM72_06575"
     CDS             2094..3572
                     /locus_tag="PKM72_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNP60487.1"
                     /translation="MWNFFKRQQNGQSSSQDIGPYSNYRMAVRLELTQRTGVFAQLAT
                     AIAEEGASLGAYDMVSATRTKVIRDITLDVQSEEHGERVMKRLNSLADVRVIASSDRI
                     FLMHLGGKIRVESKFPVKTRNTLSMVYTPGVGRVVKAIAADEKKAYSFTTKNNSIAVV
                     TDGSAILGLGNLGPLAALPVMEGKAMLFHQFAGIDAWPLCLNTQDSEEIIRTVLAVSP
                     SFGGINLEDISAPRCFDIERRLQAELDIPVMHDDQHGTAVVILGALLNALKVTRRTID
                     EITVVVVGLGAAGTACCELLLGAGVTRLKGCDKQGLVLDRPVEDLLPHRHTLRSLIQY
                     DQPTGTVQDALKDAHVVIGLSTANILKPEDLALMAKDPIVFALANPDPEILPEAAIPH
                     CRVYASGRSDYPNQCNNLLSFPGIFRGALDVQAKTINEPMLIAAAHALSKMIPDSALS
                     EEYIIPSVFDKQVVPKVAKAVSQAAIDSGMARRTKKDMEEDM"
     gene            complement(3612..4421)
                     /locus_tag="PKM72_06580"
     CDS             complement(3612..4421)
                     /locus_tag="PKM72_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNP60488.1"
                     /translation="MGYIEAVVLAVIQGLTEFLPVSSSGHLVLAQHWFGHFSETNLLY
                     DIMLHLATVTAILVYFRHDLLTLGLGYVGWTTRQGSLFQGYERRTIHYVLLASVPTAI
                     IGLGIRSIGLETLVRPSVVAIMLLTTGVILWLGRGRNSVRGIQDMSIRDAVVIGIVQG
                     VAVFPGISRSGSTIASGVLLGLDRELSARFSLLISIPAIVGATILEIGKGIDQMHEPI
                     GVYVVGMVVAALVGYGSISLIIKLVRQDHFHLFSYYLWPLGISILLWDTLK"
     gene            complement(4489..5325)
                     /gene="rfbD"
                     /locus_tag="PKM72_06585"
     CDS             complement(4489..5325)
                     /gene="rfbD"
                     /locus_tag="PKM72_06585"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNP60489.1"
                     /translation="MRQSRILITGAQGQLGQALQRQYADHEVTAWDLQDLDITEFEEV
                     KKALDQLRPHIVINAAAFTQVDEAEVKQDAAFRGNALGPRNLAVVTNDLGMTLIHFST
                     DYVFDGQQNRPYHEFDRTNPLGVYGRSKLAGESAVRTANPRHFIVRTAWLYHTVGKNF
                     PSTICRMAGREVVKVVSDQYGSPTFAPHLAQAVFQLGETDAFGTYHMAGAGGASWYEL
                     TQALYQALDIQTSVVPIATAQFPRPARRPPYAVLTSLQNPLIELPSWEEGVRDFASQW
                     KG"
     gene            complement(5322..5876)
                     /gene="rfbC"
                     /locus_tag="PKM72_06590"
     CDS             complement(5322..5876)
                     /gene="rfbC"
                     /locus_tag="PKM72_06590"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNP60490.1"
                     /translation="MKFLPTSLTEVLIVEPDVYRDPRGWFLETYHLQKYQEAGIPLPF
                     VQDNCSSSVRGTLRGLHLQRTRPQGKLIRVIRGEIFDVAVDIRKGSPTFASWVGVNLT
                     AEDFKQIYIPPGFAHGFCVLSDVAEVEYKCTDVYAPGGEVTIRWDDPRIGIQWPIERP
                     LLSTKDAAGQLLDDLCDHLPVYQP"
     gene            complement(5883..6758)
                     /gene="rfbA"
                     /locus_tag="PKM72_06595"
     CDS             complement(5883..6758)
                     /gene="rfbA"
                     /locus_tag="PKM72_06595"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNP60491.1"
                     /translation="MKGIILAGGSGTRLYPLTHAVSKQLMPVFDKPMIYYPLSTLMLA
                     GVREILVITTPHEQEGFVRLLGDGSHLGLAIRYQVQPRPEGIAQAFVIGREFIGRDRV
                     ALILGDNIFYGHGLSRYLQEAASQASGAQVFAYQVRDPERYGVVTFNDHGQATSLEEK
                     PLHPKSPYAVTGLYFYDNRVVAMADSLKPSARGELEITDVNRTYLEAGLLHVRVLGRG
                     IAWLDTGTHESLMQAAHYIQVLQERQGLMVSCPEEIAYKMGYIQSDDVSRLATKMKDN
                     GYGQYLLHLVNQGEE"
     gene            complement(6831..7925)
                     /gene="rfbB"
                     /locus_tag="PKM72_06600"
     CDS             complement(6831..7925)
                     /gene="rfbB"
                     /locus_tag="PKM72_06600"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNP60492.1"
                     /translation="METMIVTGGAGFIGANFVRMALRKTEARIVVVDKLTYAGHRESL
                     EGLWDHPRLDFVQADIADAGEMTRVMDSFSPRWLLNFAAESHVDRSIDDPYPFIHTNV
                     TGTLVLLNVVRRFLKHRTPEHQREFRFLHVSTDEVYGTLGPTGLFSEETPYAPNSPYA
                     ASKASADHLVRAFYHTYALPTLMTNCSNNYGPFQFPEKLIPLMVLNALEGKPLPIYGD
                     GQNIRDWLFVEDHCEGLLLVLQQGIPGEKYNLGGRSERSNLDVVDALCRILEDVVPSG
                     SNPSLSLRGIIRYADLKVFVTDRPGHDFRYGIDTTKVQTQLGWSPRHSFESGLQRTIE
                     WYAANRQWCQSVTANSYQRERLGLASVPST"
     gene            complement(8002..9831)
                     /gene="glmS"
                     /locus_tag="PKM72_06605"
     CDS             complement(8002..9831)
                     /gene="glmS"
                     /locus_tag="PKM72_06605"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNP60493.1"
                     /translation="MCGIIGYVGGELATPILVAGLQQLEYRGYDSAGIAIQHNGELTI
                     RRTVGKLTNLQAVLQNGAVAGTIGIGHTRWATHGRPSEQNAHPHRSGEIVLVHNGIIE
                     NFLDLRHRLELEGFRFESETDTEVIAHLLSSLTLKGHGLQEAVRLMANDLHGSYAIAV
                     MSLAEPGCIVVSRSGCPLVIGQNGKGAFLASDVTPLLAHMREVVFLEDGDVGILKNSG
                     IAILDSDGQPKNFKPVTIDWDAEAAEKGGYPDFMMKEIHEQPQVIMDTLRGRFQYETG
                     EADLPDLAMTDEELLNARRIWIVACGTSWHSGLVGKYLFEEMIRRPVQVDIGSEFRYR
                     EPMIQKDDLFIAISQSGETADTLAAAREAKHRGARVLAIVNVVGSTLAREADGVIYTR
                     CGPEISVASTKAFTGQVIALYLLALHVGRVRGTLSPEEGRWWLDHFLKLPGQVEYILK
                     QEAAVQAIANRYYQKRNFLYLGRGINYPIALEGALKLKEISYIHAEGYAAGEMKHGPI
                     ALIDEDMPVVVLSPKDRLYEKSVSNLMEVRARSAPVIAFVTEGDHALDQVADHVFAIP
                     AVHALLTPILFTVGLQLLAYHIAVLRGVDIDRPRNLAKSVTVE"
     gene            complement(9969..11480)
                     /gene="glmU"
                     /locus_tag="PKM72_06610"
     CDS             complement(9969..11480)
                     /gene="glmU"
                     /locus_tag="PKM72_06610"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNP60494.1"
                     /translation="MSGKKSTAAMILAAGQGTRMKSALPKVLHQVAGRPMLWYAATLA
                     RKVATQTVAIVVGHGSEQVQAYLEQEKADFEPFEVVIQEKQLGTGHAVQQAYPVCTRN
                     DANQADQFLILNGDTPLLTQGTLASLVDYHQSEKAGLTILTTVIPESHGYGRVVRGPS
                     GEIRRVVEHQDASAEERGISEINVGTYVVDASFLGKALSQLRPQNAQGEFYITDIIEM
                     AVQQGLKVAGWVTNDYLETTGINTREHLAIAEKEMRRRICQRLMLSGVTMLDPERVIV
                     DDGVEVGRDTSLYPGVMLEGRTVIGTNCVIHGNSRLNNCLVGNNVLIQDSCVLLDAKI
                     EEGAVIGPFAHVRPGSVIHRKGKVGNFVELKQTEVGEGSKVNHLSYLGDTVIGRNVNV
                     GAGTITCNYDGFRKARTQIEDNVFIGSDVQLIAPVTIGEGALIAAGTTVTKNVPPNAL
                     GISRVAQINKEGTAAKRREILSSSSAAHAQAKQHDDPEPSSLGSNLQQKKDSV"
     gene            complement(11516..12193)
                     /locus_tag="PKM72_06615"
     CDS             complement(11516..12193)
                     /locus_tag="PKM72_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNP60495.1"
                     /translation="MNTLRASLRRYFLTGLLVITPIWGTILILKTLFVTVDSILGTAL
                     ADLVLPDYYFPGLGILALLLLIFSAGVFATNFLGKRLVVRWEEFLDRVPIVRGIYATL
                     KSMMDILSFKQREKYNKVVMIQFPKEGHYCLSFVTGETRKEIQNLAPEPLIHVFVPTS
                     PNPTSGYFLLVPEREIVPVDMGVEEAMKLIVSGGFYSPPDKSSSIEIAKKRDRMTIKD
                     SPKVQTP"
     gene            12355..13152
                     /gene="tmk"
                     /locus_tag="PKM72_06620"
     CDS             12355..13152
                     /gene="tmk"
                     /locus_tag="PKM72_06620"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNP60496.1"
                     /translation="MKPRNHKNGKGELPSGLFITFEGTEGSGKTTQCHRLARALRAQG
                     YQVLETREPGGTPFAEAIRRLLLSYSKTKSNNEIITPECETSLILAARSQHVAHVIRP
                     ALAKGIIVLCDRFFDSTLAYQGYGRHRDITFLKHSHRFATEGLTPHLTFLLDLPTQEG
                     LTRRQQAGHQNRLDQESLDFHQRVRRGFLALAKENPQRIQKLDARQSPDILNTLIASM
                     MSHLIQTLPSTCLTQTIHQPSRPTKRVQTKGAPQHGISRSRRASTTD"
     gene            13112..14143
                     /gene="holB"
                     /locus_tag="PKM72_06625"
     CDS             13112..14143
                     /gene="holB"
                     /locus_tag="PKM72_06625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNP60497.1"
                     /translation="MAFRDLVGHQRPIKWLQTAVSTNHLGHAYLFHGEPTIGKRLTAV
                     ALAQYLHCEAPQTAPTPDACGLCRSCHQIAQATHPDFLLIQPEDTQKQNPKIPIDQIR
                     NIEHLVIYRPLLGSHKICLIDQADSMTKEAANALLKTLEDPPDHCLFLLITSRPEHLL
                     TTIRSRCITLRFAPLSSTSIYEFLRDRMGREASDARLIAAFAEGRLGSALSCDPVELK
                     VKLRQYWDLLFGEHTASASRVMDMSEGLVKSNQVPEAIHWFWAGLRDLLLLTLDHSLS
                     PTLYQDKESALRELAQQITPSSLLALIHELNQLERGQQRNLNVQIGLEQFFFHLHDQL
                     ELSNSPELA"
     gene            14233..16218
                     /gene="metG"
                     /locus_tag="PKM72_06630"
     CDS             14233..16218
                     /gene="metG"
                     /locus_tag="PKM72_06630"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNP60498.1"
                     /translation="MSNTFYVTTPIYYVNDVPHIGHAYTTIAADVLARFYRLTGHEVF
                     FLTGLDEHGQKVQQAAAKAGIDPQAHCDLLTPKFKNLWSQLHISNNGFIRTTDLQHQA
                     IVQRYLQVLYDKDLIYQAEYTGWYCTFDERFWTEKDVENGLCPDCRRPVEQVSERNYF
                     FRMSRFQSRLKEHIQQNPDFIQPDSRRNEVLGFLQKPLEDLSISRPKSRLSWGVELPF
                     DRDCVAYVWFDALVNYLSALEYISPTPSTSTFWPASLHLVGKDILTTHAVYWSTMLMG
                     LELPLPQSIFAHGWWTVNGEKMSKSRGNVVDPYAVVEEFGADAFRYFLLREVPFGQDG
                     DFSISAFGNRYNAELANDLGNLLSRTLTMLANFSNNSVPQPDAKHETDQDRQLQHAAS
                     SLFPTIDAHLRHLEFNRALETTWGLVQSANQYIDKTAPWKLAKDPGNTPRLHTVLFHL
                     TEVLRFLTLAVYPFMPQIAETMKTRLGLSTNFSTPALQEFPRWGAYLYKGQTSKGPSL
                     FPRRDSSPQESQKSPEQKMTIHPNRTHMETPQTVQSTQQQPAAGDAILISIADFQKIQ
                     LKVAKILTAERVPKSEKLLKLQVDIGTEQRQIVAGIGKKYAPEELVGRTIVVVANLKP
                     AKLMGIESQGMVLAAGDQEVLELLNMSQEIPAGTRIK"
     gene            complement(16424..>16626)
                     /locus_tag="PKM72_06635"
     CDS             complement(16424..>16626)
                     /locus_tag="PKM72_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNP60499.1"
                     /translation="IPQYNLRHTMKAGITGWAQINGLRGNTSWEKRLAYDMYYIEHWS
                     LWLDVKIMIMTLWKGLVHREAY"
BASE COUNT         4405 a         4568 c         3737 g         3916 t
ORIGIN      
        1 attcacaaac tcctcaccac ccttcactat acccttcgat ttcctgacct ctatcaggcc
       61 ttggacgaca ttgaacggca ttcatcatca tgaaaactca tcacaacaaa cagctccctg
      121 atcattttta gcggaaaaat gagacaagag aatacaccac cactccgtta ttgacggcat
      181 gcgccaccat accgggaatc acactcccgg ttcgttcgta ggcccatgcc cacaacaacc
      241 cactccagaa aaccgtcaga aacgcgatca gcccatagcc atgggccaag gcgaaaatca
      301 atccacttgc gaccatggag aggggaaaac taaatttggt tcgaagtgtc gtatagagaa
      361 ttccgcggaa aataagttct tcgaaaaagg gcgccagaag aacgaagtca atagtggttt
      421 tgatgagttc cacccgcgtt ccccaaatca gctgtgggac aaaccattcg gtccaatgga
      481 cggaagattg aaacgcatcc cccccgagca caatcagcca gtccccaaca aggccaaacg
      541 tcaccaacat cacgaccaat ggcagacagt ctttcaatcg ttgccacaca ttcgaacagc
      601 ccaccacctg tagcaacgat tgtttcctgg ttcgacacaa cagaatagcg gtcaggacga
      661 cgggaggaag atacagtaaa gcaatcccga aatcctcaat aattcctatg ccatcaggta
      721 tgaccgccac cagccccatc aacaaaatcg tcaaggcccc tccacgggct aaaacggcga
      781 ttccttcccg gaatgtccaa ggtatgcccc gtttgatgaa accggtttgg atcggtgacg
      841 ccctgcctct caggcgagca ataccaagac ggagaagcag gaccacccca atcccgatca
      901 ccacaacctc acccaccaat agcgcacgcc atttccataa cggagggtcg gtcaactgat
      961 actcttgcaa ttgaagattt gcctgtaaat ttcgatcgcc ggcctgaaca gctattcgtc
     1021 gtgcaagatg aaagtaaaac caacttgtgg ggacctcttc agctaacctt gcttgcaaga
     1081 agggataatc tggagacgac gtttcgctct ggccatatcc cacttccaaa aactcacgaa
     1141 aaagagcata aggatttctc tcactctccc aattctccac aattcggctg agttgattcg
     1201 ttaaacccgc ttctccatag atcattccca gatacaatcc atctaatggc ctggattgtt
     1261 cattaactct ttcttcatac caccgttgga tttgctcctg ggtctccccg tcctgtcccc
     1321 ccaaacgata catcacttgt tccaacaagg agagttcagg caaacgagat tcaaaaccca
     1381 gcattcggct ggcgatacgc tcaagatccc tgctttcagg tgaagccagg cgtacatcat
     1441 cagaaagcgt caccgcccaa aacaacaatc ccatacccgc cccacacacc aacaaacaca
     1501 gtacggtcag caatggggaa aatcgaaatt cttcccgaag atccggacgg gtatcggatg
     1561 tgagatcaac tgattgctct gattccatgc tcatgtctca cgggtacaag gacttttcgc
     1621 cttcgtaaaa aaccaggaag gataacatga tttccaggtg acatgctcga tacggacgcc
     1681 actgggcacc gccagcaaca tattcgccat taccgcatat tgcgtattct atacgtctaa
     1741 gaatctggtg cttttatcag gcaaagacgc gaagagaaag tggaaagagg gcgagcatcg
     1801 cagggtatcc cttggaccgc gccaagagcc acttgattgg agtcttgtta cgaagggagg
     1861 gtggggcccc ttggaaccca ctatgtaatt tgacctcggc taccgagaca aaccccggca
     1921 tcatttcgtt tatttcttaa aacctttcta gcctaaagta atggcccatt tccagccagc
     1981 agcacatatc cctaatttgg tttatccgat acaatataag aaaaaagtct gtagcctatg
     2041 agtaacccga tcaagattca ttcattgcgc aactgaagag gagggactcc ttggtgtgga
     2101 atttttttaa acggcagcaa aatggtcaga gctcctctca agacatcggc ccctattcca
     2161 attaccgcat ggccgtgcga ttagaattga ctcaacggac cggcgtattc gcacaactcg
     2221 ccacggccat cgcggaagaa ggcgccagtt tgggcgcgta cgatatggtc tcggccactc
     2281 gcaccaaagt aattcgggat attaccctgg atgtccagag tgaagaacat ggcgaacgag
     2341 tcatgaaacg gctaaattcg ttggccgatg tccgggtcat cgcctcttcc gatcggattt
     2401 tcctcatgca tttaggtgga aaaatccggg tggagagcaa gttccccgtg aaaaccagaa
     2461 ataccctgtc catggtctat acgcctggag tcgggcgcgt cgtcaaagcg attgccgcgg
     2521 atgagaaaaa agcctattcc tttacgacga aaaataacag tattgctgtc gtgaccgacg
     2581 ggtcggccat tctgggatta ggaaatctcg gccctctcgc agccttgccg gttatggaag
     2641 gcaaggcgat gctgtttcat caatttgccg gaatcgatgc ctggcctctc tgtctcaata
     2701 cccaggattc ggaagagatt atccgtacgg tgctagccgt ttctccctca ttcggaggca
     2761 ttaatctgga agacatcagt gctccccgat gctttgacat tgagcgccga ctgcaagccg
     2821 agctggatat tccggtgatg catgatgatc aacatggaac ggctgtggtc attttggggg
     2881 cactgcttaa tgccttaaaa gtcacacgtc gaaccatcga tgaaattacc gtcgtggtcg
     2941 tcggattggg agccgcaggc acagcctgtt gtgaattact tttaggagcc ggggttacca
     3001 gacttaaggg atgtgacaaa cagggcctcg tgttggatcg accagtggaa gaccttttgc
     3061 cgcatcgcca tactcttcgc agcctcattc agtatgatca gccgacggga accgttcagg
     3121 acgcactgaa agacgcccac gtggtgattg gtctttccac ggccaacatt ttaaaacccg
     3181 aagatttagc cctcatggcc aaggatccca ttgtgtttgc tttagccaat cctgaccccg
     3241 aaatcctacc cgaggcagcc attccccatt gtcgcgtgta tgcaagcggg cgatcggatt
     3301 atcccaatca atgtaacaac ctcctgtcgt tccccggcat ctttcgtgga gcccttgatg
     3361 tgcaagccaa aacgatcaat gaacccatgc tcattgctgc cgctcatgcc ctctcaaaga
     3421 tgattcctga ctctgcactc agtgaggaat atattatccc cagcgtgttc gacaaacagg
     3481 tcgtgcccaa agtcgccaag gccgtctctc aggcagcaat tgattccgga atggctcgcc
     3541 ggacaaaaaa ggacatggaa gaggacatgt aaaagcagga agtcacaaga gagggaaaaa
     3601 accagcggga tttatttcaa cgtatcccaa aggagaatac tgattccgag tggccacaga
     3661 taataggaaa ataaatgaaa gtgatcctgt ctgacgagct taataatcaa tgaaatagat
     3721 ccatagccca ccaacgccgc gaccaccatt ccaaccacat aaacccctat cggttcatgc
     3781 atctgatcta ttcccttccc aatttccaaa atcgtcgctc ccacgatagc tggtatagaa
     3841 atcaggagag aaaaccgggc cgatagttct cgatccaaac ccaacaacac tccactggca
     3901 atggttgatc cggaccggga aattccggga aagactgcca caccttgcac aataccgatc
     3961 accacggcat cccgaatgct catgtcctgg atcccccgga cactgtttct ccctcgccct
     4021 aaccagagaa tcactcccgt agttagcaac ataatggcca cgacggaggg ccggactaat
     4081 gtttcaagac cgattgatcg aatacccaat ccaatgatcg cggtgggaac ggatgcaagc
     4141 aggacataat ggattgtccg ccgctcatat ccttgaaaca ggctcccttg ccttgtggtc
     4201 catccaacat atcccaaacc caaggtgagg agatcatgtc gaaaatacac aaggatcgcg
     4261 gtcaccgtcg cgagatgcaa cataatgtca tacaacaaat tggtctcact gaaatgacca
     4321 aaccaatgtt gagccagaac caaatgtccc gaagaggata ccgggaggaa ttccgtgagt
     4381 ccttgtatga ccgcaagcac caccgcttcg atgtagccca tcgtttatga atgcctttct
     4441 tttagttggt gatcagcaga agataattca ggaaacagaa gacgactcct agcctttcca
     4501 ctgactggca aaatcacgaa cgccctcttc ccaggatggc aattcaatca aggggttctg
     4561 cagagaggtc agcacggcat agggtggtct tcgagcaggt cgaggaaatt gcgcggtggc
     4621 tataggcaca accgaggttt gaatatccaa cgcctgataa agcgcttggg ttaattcata
     4681 ccaactggct ccgcctgcac cggccatgtg ataggtccca aaggcatcgg tctcacccaa
     4741 ctggaatact gcctgggcaa gatggggggc aaaggttggt gacccatact ggtcgctgac
     4801 gactttgaca acctctcgtc ccgccattcg acaaatcgta ctgggaaagt tcttccccac
     4861 agtgtgataa agccaggccg tccggacaat aaaatgccgt gggttcgctg tccgaaccgc
     4921 cgattcaccg gctaatttac tccgcccata gacgcctaac ggattcgtac gatcgaattc
     4981 atgatacggc cgattctgct gaccatcaaa cacataatcg gtggaaaaat ggataagagt
     5041 cattcccaag tcattcgtca ccacggcaag atttctcggg cccagggcat tgccacggaa
     5101 tgcagcatcc tgtttcactt ctgcctcatc cacctgcgta aaagcagccg cattgatcac
     5161 gatgtgtggt cgtagttgat caagggcttt cttaacttcc tcaaattccg tgatatccag
     5221 atcctgcaga tcccaggctg tgacctcgtg gtcggcatat tgccgttgta gtgcctgccc
     5281 taattgcccc tgggcccctg tgattaaaat tcgactctgc ctcatggttg ataaaccgga
     5341 aggtgatcgc acaggtcgtc aagtaactgc ccagcagcat ccttggtgga gagcagtggc
     5401 cgttcgatcg gccattgaat gccaatacgt gggtcatccc atcgaatggt cacttcaccg
     5461 ccaggcgcat agacatcggt acatttgtac tccacttccg ccacatcgct gaggacacaa
     5521 aatccatggg caaaacccgg aggaatataa atttgtttga aatcctctgc cgttaagttc
     5581 actcccaccc atgaggcaaa agtcggcgac ccctttcgaa tatcgaccgc gacatcaaaa
     5641 atttcccctc ggatcacccg aataagcttc ccttgtgggc gggtcctttg aaggtgaaga
     5701 ccccgaagcg tcccgcggac cgacgatgag cagttatcct gcacaaaagg aagcgggatt
     5761 cctgcctcct gatacttttg aagatgatac gtttctaaaa accatccacg gggatcccga
     5821 tacacatcag gctcgacaat cagaacctcc gtcaggctgg ttgggagaaa tttcatgccc
     5881 ggttactcct ctccctgatt cactaaatgc agcaagtatt gcccataccc gttgtctttc
     5941 attttcgtgg ctaagcggga cacgtcatcg gattgaatgt aacccatttt gtaggcgatt
     6001 tcttccggac aggaaaccat cagaccctgt cgctcttgca aaacttgaat ataatgggcc
     6061 gcttgcatga gcgattcgtg agtccccgta tccaaccagg caatcccacg ccccaacacc
     6121 cgcacatgca gcaatcctgc ctctaaatag gttcgattca catcggtgat ttctaactct
     6181 cctctagccg aaggcttaag agaatcggcc atggccacaa cccgattgtc ataaaaatat
     6241 aacccggtta ccgcataggg tgatttggga tgtaggggct tttcctccag gctggtggct
     6301 tgtccgtgat cgttaaaggt caccacaccg tatcgttcag gatcccgaac ctgataggca
     6361 aagacttgcg cgcctgaggc ctgagaggcc gcttcctgaa gatagcgaga cagcccatgc
     6421 ccgtaaaaaa tattatcacc cagaataagc gccacccgat cccgcccgat aaactcccgc
     6481 ccaatcacaa aggcttgtgc aattccctcg ggtcggggtt ggacctggta gcggattgct
     6541 aacccgagat gactgccatc acctaacaac cggacgaacc cttcctgctc atgcggagtg
     6601 gtgatcacca gaatctcccg gactccagca agcatgaggg tcgacaacgg ataatagatc
     6661 atcggtttat caaaaaccgg catgagctgt ttgctgacgg cgtgggttaa gggatagagc
     6721 cgagttcccg atccacccgc taggataatt cccttcatca tgcccctttg tattccaatg
     6781 tcttcgtcat tctcctagta ccttgccaaa atgaaagaac ctccctgccc ctatgtcgat
     6841 gggaccgagg ctaacccaag tcgttcccgt tgataggagt tcgcggtcac agactgacac
     6901 cattggcgat tggcagcgta ccattcaatg gtccgttgca acccggactc gaaagagtgg
     6961 cgggggctcc aacccaattg cgtttgaact ttggtggtat caattccata acgaaaatca
     7021 tgaccgggac ggtcagtcac aaatactttc aggtcggcgt atcgtataat gccacgtaga
     7081 gacagggagg gattcgaacc actgggcacg acatcttcca agatccggca tagggcgtcg
     7141 acgacatcca gatttgatcg ttccgaccgc ccgccaagat tatatttttc tccaggtatg
     7201 ccttgctgaa ggaccaggag taagccttca caatggtctt caacaaacaa ccagtcccgt
     7261 atattctgac cgtccccata aatcggaaga ggcttccctt ccaaggcatt taagaccatg
     7321 agaggaatga gcttttcggg aaattgaaaa ggcccataat tattcgagca attggtcatc
     7381 aacgtcggaa gagcataggt gtgataaaat gcgcgcacta aatgatcggc actggctttc
     7441 gaggccgcat agggggaatt gggggcatag ggcgtctctt cggagaacaa ccctgttggg
     7501 cctaacgttc catacacttc atcagtcgaa acatgcagga accgaaattc ccgttggtgc
     7561 tcgggagtgc gatgcttcag gaaacgccgg accacgttta acaacaccag cgttcccgtg
     7621 acattggtat ggataaaggg atacgggtcg tcaatggacc ggtctacatg cgattcagcg
     7681 gcaaaattca acaaccacct gggagagaat gaatccatga ctctggtcat ttctcccgca
     7741 tcggcaatat ctgcctggac aaaatctaga cgaggatggt cccataatcc ttcaaggctt
     7801 tctcgatgac ccgcatacgt caatttatcc acgacaacaa tccgtgcctc ggtcttgcgt
     7861 agagccatcc gaacaaaatt ggcgccaata aacccggcgc ctccggtcac aatcatggtc
     7921 tccatgaatc aatactttcc aattgataga agccttgggg acaaaccagg ataaactccg
     7981 gtgagatatc ggaagaacgc cttactcaac cgtcacactc ttggcaagat ttctcggacg
     8041 gtcaatgtct acccctcgca acaccgcgat atgataggcc agcagttgaa gaccgacggt
     8101 aaacagaatc ggggtcaaga gcgcatggac agcaggaatc gcgaacacgt gatcggcaac
     8161 ctgatccaag gcatgatccc cttcggttac aaatgcaatc acaggggcac tcctggcgcg
     8221 cacttccatc agattactga cagacttttc atagagccga tctttaggcg ataggacgac
     8281 tacgggcatg tcttcatcaa tcaacgcaat aggaccatgc ttcatctccc ccgctgcata
     8341 cccctctgca tggatatatg agatttcttt gagtttcaag gcaccttcca gggcaatggg
     8401 ataattaatc ccacgaccca aatacaaaaa atttcgtttt tgataatacc gattggcaat
     8461 ggcttgaaca gccgcttctt gcttgagaat atactcgact tggccgggaa gcttgaggaa
     8521 atgatccaac caccaccgtc cttcctccgg acttaacgtg ccacgcaccc gtcccacatg
     8581 caaggccaat agatataagg caatcacctg tccggtaaag gctttcgtgg aagccacgct
     8641 aatttcagga ccacatctcg tataaatcac cccatcggct tcgcgagcca gagtactccc
     8701 caccacattc acgatcgcca ggacacgagc ccctcggtgc ttagcttccc gtgccgccgc
     8761 cagggtgtcc gccgtctcgc ctgattgtga aatagcaata aataaatcat ccttttgaat
     8821 catgggctcc cggtaccgga actcggatcc aatatccact tgaacgggac gtcgaatcat
     8881 ttcttcgaat aaatatttcc cgacgagccc tgaatgccag gacgtcccac aggcaacgat
     8941 ccagattcgc ctggcattga gcaactcctc atcggtcatg gccaaatcgg gaagatccgc
     9001 ttcgcccgtt tcatattgaa accggccccg aagcgtatcc atgatcactt gtggctgttc
     9061 atggatttcc ttcatcatga aatccggata gccccccttt tcagctgcct cagcatccca
     9121 gtcaatcgta accggtttga aatttttcgg ttggccgtcc gaatccaaaa tggcaatacc
     9181 agaatttttt aaaatgccga catctccgtc ttcaagaaac actacctccc gcatatgggc
     9241 caggagagga gtgacatcag aagccaaaaa tgcccctttt ccattttggc caatcaccag
     9301 ggggcatcct gaacgactga cgacgataca tcctggctca gccaacgaca ttacggcgat
     9361 ggcataactc ccatgcaggt cattggccat taacctgacc gcctcttgaa gcccatgccc
     9421 ttttaacgtc agcgaggata ataaatgcgc aataacctca gtgtcggttt cagactcaaa
     9481 tcgaaagcct tctaattcca atcgatggcg gagatccagg aaattttcaa tgataccgtt
     9541 atgaacgagg acgatctctc ccgaacggtg aggatgggca ttttgttcag atggccgacc
     9601 atgcgtggcc caacgggtat gaccaattcc tatcgttcca gcgacagctc cgttttgcaa
     9661 aacggcctgg agattggtga gcttgcccac cgtccttcga atcgtcagct ctccattatg
     9721 ttgaatcgca atcccggcgg aatcgtaccc tcgatattcc agctgttgta gcccggccac
     9781 taaaataggg gtcgctaatt cacctccaac atatccgatg attccacaca taatctaacg
     9841 ctccttacat ctcatgatgt tttttatttt tatgggaaag atcaggatgg tatcgaccag
     9901 tgaaatctgg aacgaaaaca catgagtcca gccagacatc gctcggcttc gttcatcaaa
     9961 aggggacact agacagagtc ttttttttgt tgaaggttcg atccgagtga cgacggttcc
    10021 gggtcatcat gctgtttggc ttgagcatgg gcagctgagg atgaagacag aatttctcgc
    10081 cgttttgcgg ccgtaccttc tttattgatt tgcgccactc gagaaattcc caatgcattg
    10141 ggtggaacat ttttcgtaac ggtggtccct gccgcaatca acgccccttc tcctatcgta
    10201 accggagcaa ttaactgcac atcgctccca ataaagacat tgtcctcaat ctgtgtccgt
    10261 gcctttcgaa atccatcgta gttacaagta atggtcccgg cgccgacatt cacattcctg
    10321 ccaatcaccg tatctccgag ataacttaaa tggttgacct tagacccttc tccaacttca
    10381 gtctgcttca attctacaaa attacccact ttgccttttc gatgaatgac gctaccagga
    10441 cgcacatgcg caaaaggccc gatgaccgcc ccctcttcta ttttggcgtc cagcaacacg
    10501 cacgaatcct gaatgaggac gttattgccg accaaacaat tgttcagcct cgaattgccg
    10561 tgaataacgc aattggttcc aatgacggtc cgaccttcca gcatcactcc aggatacaga
    10621 ctggtatccc ttccgacctc caccccatcg tcaacaatca cacgttcggg atccaacatg
    10681 gtgaccccgg acaacatgag acgctggcaa attcgtcgtc gcatttcttt ctcggctatg
    10741 gccagatgtt ctcttgtgtt tatcccagtc gtctccaaat aatcattcgt cacccatccc
    10801 gcaactttca ggccttgctg aaccgccatt tcaataatat cggtaatata aaattcccct
    10861 tgggcatttt ggggacgcaa ttggctcaat gccttcccaa ggaagctggc gtctacgaca
    10921 tatgtcccaa cattaatttc cgaaataccg cgttcttccg ctgaagcatc ttgatgctcg
    10981 acgacacggc ggatctcgcc tgatggcccg cgaaccactc gcccgtatcc atgtgattcc
    11041 ggtatgaccg tcgttaaaat tgtcaggcca gccttttcag attgatgata atcaacaagt
    11101 gaagccaggg tgccctgggt taataagggg gtatctccat taagaatcaa gaattggtcg
    11161 gcctggttag cgtcgttcct ggtacacacg ggataagcct gttgaaccgc atgccccgtc
    11221 cccaattgct tttcctggat caccacttca aacggctcaa aatcagcctt ttcctgttcg
    11281 agataggcct ggacctgttc cgatccatgg ccgaccacaa tggcaacggt ctgtgtcgcc
    11341 actttcctgg ccaaggtcgc ggcgtaccac agcattggac gaccggccac ttgatgcaag
    11401 actttgggca aagccgattt catccgggta ccttgccccg cagccagaat catcgcggcc
    11461 gtgctttttt taccactcat tacgcttgtc atgccattgg tcaaaggtta cttgtttatg
    11521 gggtttggac ttttggtgag tccttgatcg tcattctatc ccgttttttt gcaatttcaa
    11581 tgctagagga tttatctgga ggagaataga acccacctga cacaataagc ttcatggctt
    11641 cctcaactcc catatctaca ggaacaattt ctctctcggg aaccaggaga aaatacccgg
    11701 aagtgggatt tggagacgtg gggacaaaca catgaatcag aggctctgga gccagattct
    11761 ggatttcctt tctggtctcc cccgtgacaa acgataaaca atagtgtccc tcttttggaa
    11821 attgaatcat caccactttg ttatattttt cacgttgttt aaaggacaaa atatccatca
    11881 tcgactttag tgttgcgtaa atcccacgta cgatcggaac acggtctaaa aattcttccc
    11941 accggacaac caatcgttta cccaaaaaat tggtggcaaa gaccccggca gaaaaaatta
    12001 acagcaataa tgccaaaata cctagaccag ggaagtaata atcgggcaac accaaatccg
    12061 ctaatgcggt ccccagaata ctatccacag tcacaaacag ggtttttaaa atgagaatgg
    12121 ttccccaaat tggggtaatc accaacaggc ctgtcaaaaa atagcgtctg agggaggcgc
    12181 gaagggtatt catgaaaaga ttgccttgta cccgctacat aggcaggcag ggtcattact
    12241 taaggaagga agccacaggg atgttaccgc atcttacaat taggactgga ttcccgcaag
    12301 acttttcttg cgaatttcaa tgacttagac taggatcgct cagtgtaaat ttccatgaaa
    12361 ccaagaaacc acaaaaacgg aaaaggtgaa ctgccttccg ggttattcat tacctttgaa
    12421 ggtacagaag ggtccggaaa aactacgcaa tgccatcggt tggctcgagc acttcgtgct
    12481 caaggctatc aggtattaga aactcgagag cctgggggca ccccctttgc cgaggccatt
    12541 cgtcgtcttt tgctttccta ctctaaaacg aaatccaata atgaaatcat cactccagaa
    12601 tgtgaaacat ctctgatcct ggctgcccga tcccaacatg tggctcatgt gattcggcca
    12661 gctcttgcca aaggcatcat tgtgctctgt gacaggtttt ttgattcaac cctggcctac
    12721 cagggatacg gacgacaccg tgatatcact tttcttaagc attcccaccg atttgccaca
    12781 gaaggactga ctccacacct caccttttta ctcgatctcc cgacccaaga agggttgacc
    12841 agacgacaac aggccggaca ccaaaatcgg cttgaccagg aatccttgga tttccatcaa
    12901 cgcgtgcgtc gaggcttcct cgccctcgca aaggagaacc cccaacgcat tcaaaaattg
    12961 gatgccaggc aatctcctga tatcctgaac acgctcatcg cgtccatgat gtcacatctc
    13021 attcagacgt tgccatcgac atgcctgact caaacaattc atcaacccag ccgccctacc
    13081 aagcgcgtcc agacgaaagg ggctcctcaa catggcattt cgagatctcg tcgggcatca
    13141 acgaccgatt aaatggctcc aaacagcggt ctccacgaat catctgggac atgcctatct
    13201 gtttcatgga gaaccgacca ttgggaaacg actgaccgct gtcgcgctcg cccaatatct
    13261 gcattgtgag gcaccccaga cggcacccac acctgatgcc tgcggcctct gccggtcatg
    13321 ccaccaaatt gcccaagcca cacaccccga ctttctgttg attcaacccg aagataccca
    13381 gaaacagaat cccaaaattc ccattgatca aattcgcaat atcgagcatc tggtcattta
    13441 tcgtcctctc ctggggtctc ataaaatatg tcttattgat caggccgact ccatgaccaa
    13501 ggaagcggcc aatgccttgc tcaagacatt ggaggatcct ccagaccatt gcctctttct
    13561 cttgatcacc agccgtccag aacacctact gacgacgatt cggtcccgat gcattacatt
    13621 acgatttgcg ccactttcct ctaccagcat ttatgaattc ctgagagacc ggatgggtag
    13681 agaagcctcg gatgcccggt taatcgcggc atttgcggaa ggccgattgg gttcagcttt
    13741 gtcttgtgac cccgtagaat taaaggtgaa actccgtcag tactgggatc tgctatttgg
    13801 ggaacatact gcgtcggctt cacgggtgat ggatatgagt gaagggctgg tcaaatctaa
    13861 ccaggtaccg gaagcgattc attggttttg ggcagggctc agggacttgc tgttgctgac
    13921 cctggatcat tccttgtctc ccacgttgta ccaggataaa gaatctgctc ttcgcgagtt
    13981 ggcccaacag attacccctt cttcacttct ggccctaatc catgaactca accaactcga
    14041 gcgaggacaa caacgaaatc ttaatgtgca aatcggattg gagcaatttt tctttcatct
    14101 ccacgaccaa ttggaattat cgaattcacc agagcttgcc taacaaccat caattgcctg
    14161 cttaccccgg ttcaacgacc acccgagtcg ttttacacat gagggttcgg agttaacaga
    14221 ggatgtattg tcatgtccaa cacgttttac gtcaccactc ctatctatta cgtcaacgat
    14281 gtgcctcaca tcggacacgc ctacaccacc atcgccgccg atgttctagc ccggttttac
    14341 cggctaacag gccacgaggt gtttttcctc accggattag acgaacatgg gcaaaaagtt
    14401 caacaggctg cggcgaaggc cgggatcgat ccgcaagcac attgtgacct cctcacgcca
    14461 aagttcaaga atctctggtc gcaattgcat atctccaaca atgggtttat tcgcaccacc
    14521 gatctccaac atcaggcaat cgtacagcgg taccttcaag tcctctacga caaagacttg
    14581 atttatcaag ccgaatacac cggttggtat tgcacatttg atgagcgatt ttggacagaa
    14641 aaggatgtgg agaacggcct gtgtccggat tgtcggcgac cagtagaaca ggtgagtgaa
    14701 cgaaattact tttttcgcat gagtcggttt cagagccggt tgaaggagca tatccaacaa
    14761 aacccagatt ttattcaacc cgactcccgt cgcaatgagg tcttagggtt tctccaaaag
    14821 cccctggaag atctgtcaat ttcccgaccc aagtcacggt tgtcgtgggg cgtcgaattg
    14881 ccatttgatc gggattgcgt agcctatgtg tggttcgacg ccctggttaa ttacctgtca
    14941 gcattggagt acatctctcc aactcccagc acctcaacat tctggcccgc ctccctccat
    15001 ctggtcggaa aagatatcct gaccacccat gccgtctact ggtcaaccat gttaatgggc
    15061 ttggaactcc ctctccctca atccatcttt gcgcatggct ggtggactgt gaatggggaa
    15121 aagatgtcaa aaagtcgagg gaatgtcgtt gatccctatg ccgtggtaga agaattcggc
    15181 gcagatgcct ttcgctattt tcttttgaga gaagtgccat tcggccagga tggagatttt
    15241 tccatctcgg cattcgggaa ccggtataac gcggaactgg cgaacgatct tggcaatcta
    15301 ctgtcccgca cgctcacgat gctggcgaat ttttccaaca attccgttcc tcaacctgac
    15361 gccaagcacg aaacggatca ggatcgtcaa ctccaacatg cggcttcatc ccttttccct
    15421 accattgacg cgcaccttcg ccacttagaa tttaatcgcg cattggaaac gacctggggt
    15481 ttggtccagt cagccaatca atatattgat aaaaccgctc catggaagtt ggctaaggat
    15541 cctgggaaca cccctcgtct tcacaccgtg ttgtttcatc tcacggaggt cttgcgattt
    15601 cttacgttgg cggtgtatcc atttatgcca cagatcgccg aaaccatgaa aactcgactg
    15661 gggttgtcga ccaatttttc cacacccgca ttgcaagaat tccctcgttg gggggcctac
    15721 ctctataagg gacaaaccag taaaggccct tcgctattcc ccagaagaga ttcctcccca
    15781 caggaatccc agaagagccc tgaacagaaa atgaccattc acccgaacag gacacacatg
    15841 gaaacacctc agaccgttca atcgacccaa cagcaaccgg ccgcaggcga cgccatactg
    15901 atcagcattg ccgatttcca aaagatccaa ttaaaggtcg ccaaaatctt aacggcagag
    15961 cgagttccaa agtccgaaaa acttctcaag ctccaggtgg atatcggcac agaacagcgt
    16021 cagatcgtag ctggaattgg gaaaaaatat gccccggaag aattggtagg gcgaaccatt
    16081 gttgtcgtgg cgaatctcaa gccggcaaaa ctgatgggga ttgaatccca agggatggtg
    16141 ttagctgcag gagatcaaga ggtccttgaa cttttaaaca tgtcgcagga gatccctgca
    16201 gggacaagga tcaaatgaaa tcctctatgc ttctgttact cacaaattca gggacgtctc
    16261 cccccaaatt tttcttgaaa agaatcgctg atgccctttc acgcaatgaa gaaactacat
    16321 gctcgggaaa atgtcaggaa taattacgag gaaagatatt caacccgatt gccaaagaga
    16381 cttacatgga agggttccgt gtcgctgcac aaagagtgaa aggctaatac gcctcccgat
    16441 ggaccaaccc cttccaaaga gtcataatca taatcttcac atccagccat aacgaccaat
    16501 gctcaatata atacatatcg taggctaaac gtttttccca agaggtattt ccgcgcaatc
    16561 cattaatttg tgcccaaccc gtaattccgg ctttcatggt gtgacgcagg ttatattggg
    16621 gaatct
//
LOCUS       DAORAQ010000051        16491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_604_length_16491_cov_73.575383, whole genome shotgun sequence.
ACCESSION   DAORAQ010000051 DAORAQ010000000
VERSION     DAORAQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16491
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_604_length_16491_cov_73.575383"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PKM72_06640"
     CDS             complement(<1..863)
                     /locus_tag="PKM72_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNP60500.1"
                     /translation="MTKPTGKLTSILGVSAYYHDSAACLIQNGKIVAAAQEERFTRKK
                     HDAGFPNHAVEFCLRQGGVGLRGVDYLVFYDKPFVKFERLLETYLSYAPKGLGSFLTA
                     IPVWIKEKLFLRTLLEKAFRQVGNFEKNEKLPPLLFGEHHESHAAAAFYPSPYEEAVV
                     LCMDGVGEWATTSAWVGRGSQLTPLWEIPFPHSIGLLYSAFTYYTGFKVNSGEYKVMG
                     LAPYGEPKYVKTILDNVVDVKPDGTFRLNMDYFDYCTGLRMTNNKFDAIFGGPPRQAE
                     STLTQREMDLAR"
     gene            complement(1014..1805)
                     /locus_tag="PKM72_06645"
     CDS             complement(1014..1805)
                     /locus_tag="PKM72_06645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNP60501.1"
                     /translation="MNYYYRLGLFLLMIVCGWSSLVEARVQITTIPYNHGEVALEGVV
                     AWDDGIKGKRPGILVVHEWWGLNEYVRERAEQLAALGYVAVAVDMYGKGKVTTHPDDA
                     RQWMQQITANVEMWQARGREGLGLLQANPLVDQTRLAAIGYCFGGATVMQMVYDGAPV
                     KGVVSFHGSLPLPSASLSNKNSVKILIAHGEADPFLSQDHITKFKRALGEAGFDWHMV
                     VFGGAQHGFTNPLANQYGMEGVQYQEQANRRSWGYMKLFFDEIFR"
     gene            complement(1972..3735)
                     /locus_tag="PKM72_06650"
     CDS             complement(1972..3735)
                     /locus_tag="PKM72_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNP60502.1"
                     /translation="MGNDHHGQRNLSFILMNTSWGAFLERPAIHIPLLLVIGFLAFSS
                     NASISLFGETEGLYAIVTHTMMAAEDYVHLWLRGEPYFNKPPLFFWLQAGFIHGLGWS
                     EAALRLPSIVSSMGTMITTYFLGRLLFSGMAGFWGALVCATCYAGLWFGPLAIIDPVL
                     TFCMTVGMYAWARAYFQESSQGWYVVGFVALALGSMVKTLHALALPILVVGIFLWLRR
                     DRRVFREPYFWVGVVLSGLLLGVYYLLLGQEFRQHFFFEENLKRMITVSGDQKLSAWE
                     AYWGKRPIFWYGLVIWFDAFPWSALFPVGLFLIWKRRPWLESPKELWVFLWVVVYFVA
                     LSLAPEKHERYLMPLLPAFGLLVGYVYHRLLYETSASDGWGPLRGMLGCLGIVFVVAV
                     ILGPILIQKKWHVPLDIIPLALRLGLGILGLMMIGLAMKNYLRMGLVGVGVLGIALMV
                     TVTGFIIPGIQAEGSPRLAFDENQRRLNNQTDPILVFQSWDWREDEDEFYWDYLHGYS
                     RIVGKGLGDFLALEELKRDVQQSTRLVMMTKDQYQRVISGDPNLNAIVLFEFYRSKKN
                     IVLLSLDWRTQLADSLEPQKQ"
     gene            complement(4033..4109)
                     /locus_tag="PKM72_06655"
     tRNA            complement(4033..4109)
                     /locus_tag="PKM72_06655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4073..4075),aa:Arg,seq:tcg)
     gene            4342..4653
                     /locus_tag="PKM72_06660"
     CDS             4342..4653
                     /locus_tag="PKM72_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60503.1"
                     /translation="MIRTEQLAHDTIMFHVRGQFNKQVAKELSLSVFRSYHLGFKTFL
                     CNLYLVMVFDKDAHYHLTLIGEGLRVKGRNWRVIRPPSSSGNQLILRTNLQLLTPANL
                     N"
     gene            complement(5162..5602)
                     /locus_tag="PKM72_06665"
     CDS             complement(5162..5602)
                     /locus_tag="PKM72_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60504.1"
                     /translation="MKNEAKLEPGQVVDTLGELIAGLAAFAAKVPAKSMLALSAGIRP
                     TPEAVDAYEITVYRFRDRVGLTYKTLPPLFVDSLEAFEAGKVFDAVPPLLQCVEQLVE
                     LHNQETIKFTPPQQQRLRDYHRRLEKLVPEATQSEVDLPTPESY"
     gene            complement(5637..6608)
                     /locus_tag="PKM72_06670"
     CDS             complement(5637..6608)
                     /locus_tag="PKM72_06670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNP60505.1"
                     /translation="MPHVATRTEFIVTAGESPKRLDHFLANREPYFSRTALQRLIEDG
                     HITVGGQVVKPSHKVRPGDCVVLVVPRPEPVEINPEPIPLVILYEDEFLLVLNKPAGL
                     VVHPAPGHWTGTLVNALLHHMQSGEGHLSSIGGKERPGLVHRLDKETTGILVVAKNDQ
                     AHRLLAGQFKAHSITRVYEAIVWGGPKTREGRIELSIGRDTKDRKTFSARTTNPKESL
                     TSFRVRERFGSAAAHVELFPGTGRTHQLRVHLKAIGCPILGDQTYGGKKVMLVDGMAI
                     PRVMLHAKLLGFVHPVSNKMVTFEAELPEDMATVLNALKGKEDLLLS"
     gene            complement(6642..7094)
                     /locus_tag="PKM72_06675"
     CDS             complement(6642..7094)
                     /locus_tag="PKM72_06675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNP60506.1"
                     /translation="MADSAATESSGKILLEVVTPEHRLLSKEVDYVSAPGSEGDFGVL
                     PGHCHFLTTLRIGELHYRVGEQTEYMSVLWGFAEVTPTKVTILAEIAEKAEDINVERA
                     EDAVRKAEERLERGGLPSEVEEARVSLEKARLRQKIAGRRQQGGSRLL"
     gene            complement(7094..8548)
                     /gene="atpD"
                     /locus_tag="PKM72_06680"
     CDS             complement(7094..8548)
                     /gene="atpD"
                     /locus_tag="PKM72_06680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNP60507.1"
                     /translation="MVGTDLGIGKVIQVIGPVVDVEFPPGKLPNIFNAITITNNSGDQ
                     SGQPDKVTLEVAQHLGENRVRAVAMSSTDGLVRGLEVRDTGAAISVPVGKETLGRVVN
                     VLGDPVDGFGEVKTQKRWSIHRPAPALEDQETKTEILETGIKVIDLLEPYAKGGKVGL
                     FGGAGVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQDSKVIDPHDFT
                     KSKAALVYGQMNEPPGARLRVGLTGLTIAEYFRDEEHQDVLLFVDNIFRFTQAGSEVS
                     ALLGRMPSAVGYQPTLGTEMGTLQERITSTKKGSITSVQAIYVPADDLTDPAPATAFA
                     HLDATTVLSRQLAELGIYPAVDPLDSTSRILDPHILGEEHYQVVQRVQGTLQRYKDLQ
                     DIIAILGMDELSEDDKQLVARARKLQRFLSQPFHVAEAFTGSPGKYVKLVDSVRSFKE
                     IVDGKYDHLPEQAFYMVGPIEEAIEKAEKLGYKK"
     gene            complement(8742..10262)
                     /gene="atpA"
                     /locus_tag="PKM72_06685"
     CDS             complement(8742..10262)
                     /gene="atpA"
                     /locus_tag="PKM72_06685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNP60508.1"
                     /translation="MQIKAEEISSIIKEKIKGFEQRVDVKEMGYVIQVGDNIAKVYGL
                     EGAMAGELLEFPGGVYGVALNLEEDSVGAVLLGEDVGIREGDPVKRTGRIAEVPVGEA
                     LVGRVVDAIGKPIDGKGPINTTETRLIEVRAPGVVDRQSVGEPLQTGLKAIDAMIPVG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLGVFCFYVAIGQKRSTVARVVKTLEDHGAM
                     EYTTVVSATASDSASLQFFAPYAGVTMAEYFRDNGKHALIVYDDLSKHATAYRQLSLL
                     LRRPPGREAYPGDVFFLHSRLLERAAKMSDEKGAGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLAADLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFSQFGSELDKATQAQLARGARMVELLKQDQYKPLPVADQVLSIFAGVNGFLDNVPV
                     NKIREFEQNLLAFIKEKYPNIREEVVTKKKIDEEFGGKLKNIVSEFKQSKGYDQAG"
     gene            complement(10266..10805)
                     /gene="atpH"
                     /locus_tag="PKM72_06690"
     CDS             complement(10266..10805)
                     /gene="atpH"
                     /locus_tag="PKM72_06690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNP60509.1"
                     /translation="MNKSTIGRRYASALFQLLDKSGVEPARSALSALQQGLEETPALK
                     HVLASPVFNFEEKQAVLNELSRRMEAPPVMRDFLTQLLKKNRAMLLPEISEAFADLAS
                     QQQGVQQVCVGSAKPLQAAEKEQIKQNLSQSLHHGVEVAFEVDPHFIAGLRIQIGSLV
                     FDNTVSGRLAGMHDQLTKG"
     gene            complement(10939..12090)
                     /gene="mnmA"
                     /locus_tag="PKM72_06695"
     CDS             complement(10939..12090)
                     /gene="mnmA"
                     /locus_tag="PKM72_06695"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNP60510.1"
                     /translation="MPFSVSKRVVLGMSGGVDSSVAAKLLKQQGHEVIGVTLKVWEEE
                     DESSVSKRWEERGCCKVGMARHVAELLGISHQVIDTRERFQKGVIDDFIGGYIEGTTP
                     NPCVRCNERVKFGGLFQVADDLGADFIATGHYANIRHDEAGTPFLVRGQDVKKDQSYF
                     LYRIPPSWLSKMMFPLGEMEKPDVWKEAEAMGLPVDELKESQEICFVTQGDYRQFLKQ
                     HAPQASIPGTFVDGKGKELGQHQGVAFYTPGQRKGLGISGGSRLYVQRVEPEKNMVVL
                     GPAEDLDSRECLVSDLNIFSPETLRNTPKIDVKFRYSSPPVSAIHRWAGSTSMTIMFD
                     SPVRALSPGQSAVFYLGDRVLGGGIIQGPTHKKGIQMCEEPISVESLPR"
     gene            complement(12090..12566)
                     /locus_tag="PKM72_06700"
     CDS             complement(12090..12566)
                     /locus_tag="PKM72_06700"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNP60511.1"
                     /translation="MWGEKDKKKDLGFGQEFYTFLGKGVDFKGKAQFEGTVRVDGNFE
                     GEITIDDTLIIGESAVIKGTITGGTIVNSGRIEGVITAKSKIQLLKPAVLIGDVHAPI
                     FAMEEGVYFQGECDMGSAPKLEAPIDNKGIPNGQGKTIPKVEQKIEKKLETEPLSR"
     gene            complement(12673..13173)
                     /locus_tag="PKM72_06705"
     CDS             complement(12673..13173)
                     /locus_tag="PKM72_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNP60512.1"
                     /translation="MFFNKKEENGQDAAEMSQGEQKEPAVVDGPVVSDSQPESGDIIA
                     FVGEEVTFKGTIRYQGTVRVDGRLEGEIYTDGNLIIGQKAVIAAKIHAGTIMCQGQLT
                     GDVEAKHRVKLLAPAVFVGKITAPLLSMDEGVLFNGTCNMPQKNERKGKETKLVISVE
                     EAVKVK"
     gene            complement(13331..14188)
                     /locus_tag="PKM72_06710"
     CDS             complement(13331..14188)
                     /locus_tag="PKM72_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNP60513.1"
                     /translation="MEKKALGKGLQALLPEKKTLVWKVEQDGQAVSMVPLDQILPNRY
                     QPRTTFFEAELDELAQSVKEHGVLQPIVVRRKGDGMYELIAGERRFRAAKIAGLSTIP
                     VLVRNSNDEKALAIALVENIQRENLNPMEEAKAYSRLMGEFGLTQDLVSRSVGKDRSS
                     IANIIRLLSLPKDVQEYIETGAISLGHAKVLAGLPTPAAQLQLARKIVSEQLSVRQIE
                     QMIAVHKKGKFAKSKKEEPNRFRNLEEQFRKRLGTKVQVKSGNRGGQVIIHYFSDDEL
                     DRITSLVLE"
     gene            complement(14166..14933)
                     /locus_tag="PKM72_06715"
     CDS             complement(14166..14933)
                     /locus_tag="PKM72_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNP60514.1"
                     /translation="MKQTIAIANQKGGVGKTTTSINLCAALAISGKSVLLIDLDPQAN
                     ATSGLSIEPQGVTIYECLMERRLIEKVILPTDISGLSVVASKGDLVGAEIELTNLPDR
                     QTILKSVLDGILDYDFIIIDCPPAFGLLTINALVAASGLIIPVQCEYYAMEGLGRLLG
                     NVERIRESFNTNLEIQGIVLTMYDSRINLSRQVQDEIRGFFADKVFQTVIPRNVALAE
                     APSHGKPVLSYNAASSGAKAYLDLAKEILRNGKESIR"
     gene            complement(14991..15650)
                     /gene="rsmG"
                     /locus_tag="PKM72_06720"
     CDS             complement(14991..15650)
                     /gene="rsmG"
                     /locus_tag="PKM72_06720"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNP60515.1"
                     /translation="MEQSDQSFNFIDHFKRGAQKLGLSFTEATLGKFGLYYDELCRWD
                     RSINLTGLRTDRERTILLFVDSLAGHLALGNKADAKIVDIGTGGGFPGIPLKLALPAL
                     DVLLMEPRSNKTAFLHYIIGKLELPKTSVLQVRLEDFDSMVVDDEKCDFAICKGVNVD
                     HILPYLEHILKKTGKLVVFRSKSIDNNSRLDGMEVFEEFSYELPFEYGSRVLSILKFA
                     P"
     gene            complement(15766..>16491)
                     /locus_tag="PKM72_06725"
     CDS             complement(15766..>16491)
                     /locus_tag="PKM72_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNP60516.1"
                     /translation="QGIMAGINAALQVKNDRPLILDRSQAYIGVLIDDLITKDAREPY
                     RMFTSRAEYRLLLREDNADFRLMEEGYRIGLVSQGTFDTFQEKKGKIEREINRLHATA
                     PKSSGLVHDDIIPMESFAPNSSLAQLLRRHEVSYHHLAQFLNGTKEDDPDVVEAVEIT
                     IKYEGYIKRQEQLIGQFKKLEHRHVPDDFNYDDIKGFSNEVVEKLKKVRPSSIGQASR
                     ISGMTPAAISLLLVAVEKHKKAS"
BASE COUNT         4379 a         4382 c         3459 g         4271 t
ORIGIN      
        1 cgggccagat ccatttcccg ctgggtgaga gtactttccg cctgacgcgg aggaccacca
       61 aatatggcgt cgaacttatt gttggtcatc cgtaatccgg tacagtaatc gaaatagtcc
      121 atattgagcc ggaaggtgcc atccggcttc acatccacca cattatccaa aattgttttc
      181 acatacttag gctctccata gggagcgagc cccatgactt tatattcacc ggaattgacc
      241 ttaaatcccg tatagtatgt aaaggcggaa tacagcagcc cgatggaatg agggaaggga
      301 atttcccata gaggcgttaa ttggctcccg cgtccaaccc atgctgaagt ggtggcccac
      361 tcgccgaccc cgtccataca caggaccacg gcctcctcat aaggggaagg gtaaaaggcc
      421 gcagcggcat gcgattcatg gtgctcccca aagagcaaag gtggcaattt ctcatttttt
      481 tcgaaattac ccacctgacg aaaagccttt tctaataaag ttcgcagaaa cagcttttct
      541 ttgatccaaa ccggaatggc ggtcaaaaaa gatcctagcc ccttcggggc ataggacaaa
      601 taggtttcca acaaacgttc aaatttgaca aaaggtttgt catagaacac cagataatcg
      661 accccgcgca ggccaacccc cccttgccgc agacaaaact ccaccgcatg gttcgggaac
      721 ccggcatcat gtttctttcg cgtaaaccgt tcttcttggg cagccgctac aatcttgcca
      781 ttctggatga gacaagccgc actatcgtga taataggccg agacgcccaa aattgaagtc
      841 aatttgccgg ttggcttggt catgtattcc ttaaattagg ggctttcctt agtaattgag
      901 actggtgtta ccaggcagaa aaacctcgaa ccctactttt tcggtgaaaa tcaaagggac
      961 ttaagagatc ctcttttctg ttcatactat cgatagaaaa gagcacactc aactcatcga
     1021 aagatttcgt caaagaacaa cttcatatac ccccatgatc gtcgattggc ttgttcctgg
     1081 tattgtaccc cctccatgcc atattgattg gccaaaggat tagtaaatcc atgttgggcg
     1141 cctccaaaaa ccaccatgtg ccaatcaaac ccagcttcgc ctaatgcacg tttaaatttt
     1201 gtaatgtggt cctggctaag aaagggatca gcctctccat gggcaatgag gatttttact
     1261 gaattcttat tagaaagaga tgcggacgga agagggagcg acccatgaaa actcacgact
     1321 cctttgaccg gtgccccatc atacaccatt tgcataaccg ttgcccctcc aaaacaatac
     1381 ccaatagccg ctaaccgggt ttggtctacc agggggttag cctgaagaag ccctaatccc
     1441 tctctccccc tggcctgcca catctctaca ttggcggtga tctgttgcat ccattgcctg
     1501 gcgtcatcgg gatgagtggt cacttttccc tttccataca tatccacagc caccgccaca
     1561 tatcccaagg ccgccaattg ttccgcacgt tctctgacat attcattcag gccccaccat
     1621 tcgtggacaa ccaaaatccc cggacgctta ccttttatcc catcatccca ggcaacgacc
     1681 ccttccaaag caacttctcc atggttatag gggatagttg taatctgtac tctggcttcc
     1741 actagcgaag accacccaca cacaatcatg aggagaaata gacccaatcg atagtaatag
     1801 ttcatgattt ctactttttt gatggattgg tataaaatat ggatcggaat accgactcag
     1861 aaatgataac gatctagaac tatttcttcc attcattgac ctgaattgct tcaggaaggg
     1921 ctagatacta aattttacgg taaatttagc agttggcaat ctttttggca ttcattgctt
     1981 ctggggttcc aaagaatccg ccagctgtgt tctccaatcg agggaaagga gcacaatgtt
     2041 cttttttgat cggtagaact caaacaacac gatggcattc aaattcggat caccggaaat
     2101 aactcgttga tactggtctt tcgtcatcat caccaaacga gtagattgtt gaacatccct
     2161 tttcagttct tcaagcgcca ggaaatctcc aagacccttt cccacaattc gtgaataacc
     2221 gtggagatag tcccaataaa attcatcttc atcctctcgc caatcccatg actgaaacac
     2281 caatataggg tcggtctgat tattcagacg ccgttgattt tcgtcaaagg ccagcctggg
     2341 agaaccttcc gcctgaatac ctgggataat aaaccctgtt actgtcacca taagggctat
     2401 gcccaacacc ccaacaccca ccagacccat tcgcagataa ttcttcatgg ccaggccaat
     2461 catcatcagg cctaagatac ctaatcccag tctaagcgcc agaggaatga tgtccaacgg
     2521 aacatgccat tttttttgta tcaggatagg tccgagaatt actgcaacaa caaaaacaat
     2581 acccaaacat cctaacatgc ccctcaacgg gccccatcca tctgatgctg acgtttcata
     2641 tagcaatcga tgatacacat accctaccaa taaaccgaat gctggcagga gaggcatgag
     2701 ataccgttca tgtttttcag gagccaaact caacgccaca aaatatacca ccacccaaag
     2761 aaagacccac aattcttttg gcgactcaag ccatggtcga cgtttccaga tgagaaaaag
     2821 gcccacagga aataaggccg accaggggaa ggcatcgaac cagattacca gtccatacca
     2881 aaaaatggga cgtttccccc aataggcctc ccacgcagaa agtttttgat ctcccgagac
     2941 ggtgatcata cgtttgaggt tttcttcaaa gaaaaaatgt tgtctgaatt cctggccaag
     3001 caacaggtaa tagacgccca gcaacaaacc actcaagacg actcccaccc aaaaatacgg
     3061 ttcacgaaac acccgcctgt cccggcgcaa ccataagaat attcccacca ccaaaatcgg
     3121 tagggctaag gcatgtaacg ttttgaccat cgagcccaaa gccaaagcca caaaaccgac
     3181 gacataccac ccttgagacg actcctgaaa atacgcgcgg gcccaggcat acatacccac
     3241 cgtcatacaa aacgttaaca caggatcaat aatggccaag ggaccaaacc acaagcccgc
     3301 ataacaggtc gcgcagacca gtgcgcccca aaatcctgcc attcctgaaa ataacaaccg
     3361 gcccaagaaa taggtcgtga tcattgttcc catactggaa acaatcgatg gtaaccgtaa
     3421 cgccgcctca ctccatccca acccatgaat aaatcccgcc tgaagccaaa agaaaagagg
     3481 cggtttatta aaatacggtt ccccccgcag ccacagatgc acataatcct ccgccgccat
     3541 catggtgtgg gtgacaatgg catacaaacc ctctgtttcc ccaaacagag aaatcgaggc
     3601 attggaacta aacgccagaa aaccaatgac tagaagaaga ggaatatgga tagcagggcg
     3661 ctcaaggaag gcaccccagg aggtattcat cagaataaaa gagagatttc gttgcccatg
     3721 atggtcattt cccactaatt tagcgggaac accctacaaa aaaaccaccg aggttgttaa
     3781 cccaatcggc tatttgaacc actcatttct ttgaatcagg gcatcggcat caacttggga
     3841 atggtgattg ggcaacaata cttttatgtt tgcccctatt gaagttctgc catgaagcag
     3901 ccggagggaa ttaatacaaa aggagaattg acagtcccta attaatacgt gaataactct
     3961 aaaaatcacg tttacgtgtt gtaacctacg agtggtggta ttaaatcatc aagtcaacag
     4021 aggaaattta aatggtgcgc ccggagggac ttgaaccccc aaccctcggc tccgaaggcc
     4081 gatgctctat ccaattgagc tacggacgca taaggagttg actttataca tcccgccagg
     4141 gtccccgtca accaggctgt catctcttca gggttcctct agctgtttcg gtttctttcc
     4201 atccactaaa agtgatttga tcaactagta aatttgacca gtttggccca tccgtctggg
     4261 gaatagtcgc agaagctcct gttgggttga aataggcttg agtaagaaat ctttgcaaat
     4321 tttccaaaag tcaggaatca catgatacgt accgaacaac tggcccacga caccatcatg
     4381 tttcatgtgc gagggcagtt taataaacag gttgccaaag aattgagttt gtcggtcttt
     4441 cgttcctatc acttagggtt caagacattt ctttgcaatc tgtacctggt aatggtgttt
     4501 gataaggatg cacattacca cctgacattg attggggaag ggttacgagt taaaggaaga
     4561 aactggcgag taattagacc cccctcttcg agcggaaacc agcttatcct ccgcacgaat
     4621 cttcagttgc ttactcctgc aaacttgaat taattttcct gcttaggttc taactccaag
     4681 tgcaacaggg agttcatagc tttttctatc aggcggttcg taacctatga gggtttacca
     4741 ttggccaaga gctattggac tgtccccacg accataatct ctttctaggt tctcccagtc
     4801 actaaggcca ttgcaatatc aggcttatcc cttttcaaag aatgttgcac ttggagaaac
     4861 cgccctgtta aaaaaaaaga cgttaccgac tatcttccct cggtgtaaat aaaaacctcc
     4921 atgtccggct ctcacttaag cccttcttga actcacctga taactataaa ttttgcccct
     4981 ctccaatttt ttgtaatccc tgctgacttc cggccagaat gcatatcatt tgtggcgggg
     5041 atagagattc gattaccaat ttgtggaatg atggaagggt ggagttaaag aaaagaaaac
     5101 gggagatcgt tccgcttgat ctcacaggga atttctcttc gactattccc caatgtcatg
     5161 attaatacga ttcaggggtg gggagatcga cttcggattg ggtggcttcc gggacgagtt
     5221 tttctaatcg gcgatgataa tcacgaagac gttgctgttg aggtggagtg aatttaatag
     5281 tttcctgatt gtgcaattcc accaactgtt cgacgcattg taacagggga ggaacggcgt
     5341 caaatacttt ccccgcctca aaggcttcta aggaatcgac aaataacggt ggaagagttt
     5401 tgtacgtcag tccaactcgg tcacgaaatc gatagaccgt gatttcatag gcatcgactg
     5461 cctccggagt cggacgaatt ccagcagaca atgccagcat ggacttggcc ggtaccttgg
     5521 cggcaaaagc cgctagacca gcaatcagtt ctcctaaggt gtcaaccacc tgcccaggtt
     5581 ccagcttggc ctcgtttttc ataggttcaa atctactcca atcgcttggt tgcttgtcaa
     5641 gacagcagta ggtcttcctt gcccttcaag gcattcagta ccgtcgccat atcttccggc
     5701 aactctgctt caaatgtcac cattttatta gaaacaggat gcacgaaccc gagaagcttt
     5761 gcatgtaaca tcacccgggg aatggccatc ccgtctacca acataacttt tttcccacca
     5821 taggtttgat cacccaggat cggacatccg atagccttga ggtgaactcg tagctgatgc
     5881 gtgcgtcctg taccagggaa taactccaca tgggcggccg ctgacccaaa ccgctccctc
     5941 accctgaaac tggtcaacga ctccttggga ttggttgtgc gtgcggaaaa agtttttcgg
     6001 tctttggtat cccgtccgat cgacaactca atccggcctt cccgtgtctt tggtcctccc
     6061 cacacaatcg cttcataaac tcgagtgatg gaatgggctt taaattgtcc tgccaataat
     6121 ctgtgagcct gatcattttt cgccacgact aagatacctg tcgtttcttt gtccaaacga
     6181 tgaaccagcc ccggacgctc cttccctcca attgatgaga gatgcccttc accgctttgc
     6241 atatgatgca ggagcgcatt cactaaggtc ccggtccaat ggcctggtgc cggatgaacg
     6301 acaaggcctg caggtttatt gagaaccaac aggaattcat cctcatacag gatgacaagg
     6361 gggataggtt caggattaat ttccacgggc tctggacgtg gtacgaccag cacaacgcaa
     6421 tcaccaggcc taaccttgtg gctgggtttc acaacctgac caccgacggt aatatggccg
     6481 tcctcaatta atcgttggag agctgtccgg gaaaaatagg gttcgcggtt ggcaagaaaa
     6541 tgatcgaggc gcttgggcga ttcgcctgcg gtgacgatga actccgtccg cgtagcgaca
     6601 tgcggcatgg caggtaaaaa cgaacggggt ggtcaaaaaa tctataacag gcgggaaccg
     6661 ccctgttggc ggcggccggc aatcttttgt cgaagacggg ctttttccaa actgacccga
     6721 gcttcttcta cttccgatgg caaaccacct cgctccaaac gctcctctgc ttttcgaacg
     6781 gcatcctcgg cgcgttccac attaatatcc tccgcttttt cggcgatttc ggccagaatc
     6841 gtcacctttg tcggcgtgac ttccgcaaat ccccaaagta cagacatata ttcggtctgt
     6901 tcacccacac ggtaatgaag ttcgccaatc cgcaaagtgg tcagaaaatg acaatggcca
     6961 ggaagcaccc cgaaatcgcc ttcgcttccg ggagccgaca catagtcgac ttccttactg
     7021 agcaagcggt gctccggtgt gactacctcc aaaagaatct tccctgagga ctctgttgca
     7081 gcagaatcag ccattatttt ttatatccaa gcttttccgc tttttcaatg gcctcttcaa
     7141 tcggtccaac catatagaag gcctgctctg gtaaatgatc atatttacca tccacaattt
     7201 ctttgaagct tcgaaccgaa tcaacgagct tgacgtattt cccaggggat cctgtaaagg
     7261 cttcggccac atggaacggt tgagagagaa accgttggag ttttctggcc ctcgccacta
     7321 attgtttgtc gtcttccgac aattcgtcca tacctaaaat cgcgataata tcctggagat
     7381 ctttataccg ctgcaaggtg ccttgaaccc gttgcaccac ttggtaatgt tcctcaccca
     7441 aaatatgggg atctaaaatt ctggaagtgg agtccaaagg atcgaccgca ggatagatgc
     7501 ccaactccgc cagttgccgg gacaacaccg tggtggcatc caaatgggca aatgccgtag
     7561 ccggggcggg gtcagtcagg tcatcggccg gcacataaat cgcttgaacg gacgtgatgg
     7621 atcccttctt ggtcgacgtg atccgttctt gcaaggtccc catctccgta cccagcgtcg
     7681 gttgatagcc cacggctgat ggcattcgac cgagcaaggc cgaaacctcg gatcctgctt
     7741 gagtaaatcg gaaaatgttg tcgacaaaaa ggagcacgtc ctgatgctcc tcatcacgaa
     7801 aatattccgc gatggtcagt ccggtgagac ccacgcggag acgcgctccg ggtggctcat
     7861 tcatctgacc ataaaccaaa gcggcttttg atttggtgaa atcatgggga tcaatgacct
     7921 tggaatcctg catttcatgc cacagatcat tgccctcacg ggtccgctca ccaactccgg
     7981 caaaaactga aaacccgcca tggtggagag cgatgttatt gataagctcc ataatgatga
     8041 cggttttacc cacaccggca ccaccgaaca ggccgacctt tccccctttg gcatatggct
     8101 ccaacaggtc gatgacttta attccggttt ccaatatttc tgttttggtt tcctggtcct
     8161 ctaatgcagg agccggacga tgaatagacc accgtttttg cgttttgact tcgccgaagc
     8221 catcaacggg atcccccaag acattaacca ctcgtcccag agtttccttg cctacgggaa
     8281 cagaaatagc ggcaccggta tcccttacct ccaaaccccg gaccaaccca tccgttgaag
     8341 acatggcgac cgccctcaca cggttttccc caaggtgttg ggctacttcc aatgtgacct
     8401 tatccggctg tccgctctga tctcctgaat tattggtgat cgttatggca ttaaaaatat
     8461 ttggaagttt tcctggcgga aactcaacgt ccaccaccgg tccaatgact tgaatgactt
     8521 ttccaattcc gagatccgta cccaccatag actccttggt tgaaaaagat tggtgaacgg
     8581 cacacggagt cccgtgcccg gccaggatca accttcgaat tatttagagg actctgagcc
     8641 tcccacaata tacatgttca cggcattagt tattttctga gtgtatttaa tagaagaaat
     8701 tttttgtcga agactttgaa gactcggcat agcaaataac tttatcccgc ttgatcgtag
     8761 cctttggact gtttaaattc ggaaacgatg ttttttaact tacccccaaa ctcctcatca
     8821 attttctttt tggtcacaac ctcttccctg atattggggt acttttcttt aatgaaggct
     8881 aaaaggtttt gttcaaattc cctgatttta ttcacaggaa cattatcaag aaacccattc
     8941 actcccgcaa aaattgacaa aacctgatct gccacaggca ggggtttgta ctgatcctgt
     9001 ttgagcaact ctaccatgcg tgcacccctg gccaattggg cttgggtggc tttgtccaat
     9061 tcacttccaa attgcgaaaa ggccgccatt tcacgatatt gggccaagtc aagcctgagc
     9121 gtccctgcaa cttgtttcat cgttttaatc tgggccgaac cacccacgcg ggatactgac
     9181 aacccaacgt taatcgctgg ccgaatacct gaatagaaca agtcggcagc cagatagatc
     9241 tgcccatcgg taatagaaat cacattcgtg ggaatatagg cagagacgtc acctgcctgc
     9301 gtttcaataa tcggcaatgc agtcaaactg cctgcgcctt tttcatcact catcttggct
     9361 gcccgctcta ataagcggga atgcaaaaag aacacatcac ccggataggc ttcacggcct
     9421 ggtggacgtc gaaggagaag ggaaagctgt cgataggcgg tcgcatgctt ggacaaatcg
     9481 tcatacacta taagcgcatg cttcccgttg tcacggaaat attccgccat agtgacaccg
     9541 gcatacggtg caaaaaattg tagcgaagcc gaatcgctgg ctgtggcaga caccaccgtg
     9601 gtgtactcca tggctccatg gtcttccaag gttttcacca ctcgagctac cgtcgatcgt
     9661 ttttgcccga tagccacata aaaacaaaag acacccagac ctttttgatt gatgatggtg
     9721 tcaacggcaa tggctgtttt cccagtctga cggtctccaa taattagctc ccgttggcct
     9781 cgcccgacag gaatcatggc atcaatggcc ttgagcccag tttgaagtgg ttctccgact
     9841 gattgccgat caactacacc aggtgcccga acttcgataa ggcgtgtttc cgtggtattg
     9901 atcggtccct ttccatcaat gggcttccca atggcatcga ccacgcgacc gaccagcgcc
     9961 tccccaacag ggacctcagc aattcgcccg gtgcgcttca ccggatcacc ttcgcgaatc
    10021 cccacatctt cacccaacag gaccgctccc acactatctt cttccaggtt caatgccacc
    10081 ccgtatactc caccaggaaa ttccaacaac tcccctgcca tcgccccttc cagaccatag
    10141 accttggcaa tattatcacc tacctggatg acgtaaccca tttctttgac gtcaacccgt
    10201 tgctcgaatc cttttatttt ttccttaatg atggagctga tttcttctgc tttgatctgc
    10261 atggattatc ccttcgtcaa ctgatcgtgc ataccggcta gtcggccaga caccgtatta
    10321 tcaaatacca ggctgccaat ttgtattctc aatcccgcaa taaaatgggg atccacctca
    10381 aaagccactt ccaccccatg gtgcagcgat tgagacaagt tttgcttgat ctgttctttc
    10441 tcggccgctt gtaatggttt ggccgagccc acacagactt gctgaactcc ctgttgttga
    10501 gatgccaaat ccgcaaacgc ctctgaaatt tcagggagca acatagcccg attctttttc
    10561 aacagttgag tgaggaaatc ccgcatgact ggaggggctt ccatccgtcg gcttaattca
    10621 ttcagaacgg cctgtttttc ctcaaaatta aagacaggtg aagcgaggac atgcttcagg
    10681 gcaggggttt cctccaagcc ctgttgcaat gcgcttaagg cacttcgtgc gggctcaaca
    10741 cccgatttat cgagcagttg gaaaagcgcc gatgcatatc ggcgcccaat ggtgctttta
    10801 ttcacagttt acagagattc caggcagcca cgggttgaac ggaactgggc gtgtgctaag
    10861 cgggtgaaag tagcatgagt gataaaggct gtcaaccgca aagagcggat gtaaagggat
    10921 tttttaacta attccgtatt aacgtgggag tgactcaacg gagatgggct cctcgcacat
    10981 ctgaatgcct tttttgtgag ttggcccttg aatgattcct cctcccaata cacgatcacc
    11041 cagataaaat acggctgact ggcctgggct taaagcccga acaggcgaat caaacattat
    11101 tgtcatcgag gtggagcctg cccatcggtg aatagctgag acgggaggag aactataacg
    11161 aaatttcaca tcaatttttg gagtatttct caatgtctcc ggggagaaaa tattcaaatc
    11221 tgaaaccagg cattctctgc tatctaaatc ctcggcagga cccaaaacta ccatattttt
    11281 ttccggttcc acgcgctgta catacaaccg gcttcctccg gaaataccca atccttttcg
    11341 ttgccctggc gtataaaacg cgacgccttg atgctgccct agctccttac ccttcccatc
    11401 cacgaaagta ccaggaattg aagcctgagg tgcatgctgc ttgaggaatt gacggtaatc
    11461 accttgcgtc acaaagcaaa tttcctggct ttctttgagt tcgtccactg gcaggcccat
    11521 ggcctccgct tccttccaga cgtcaggttt ttccatttcc cctaaaggga acatcatttt
    11581 tgagagccaa ctgggtggta tacgatagag aaaataagat tggtcttttt tgacatcctg
    11641 cccacgaacc aggaaagggg tccctgcttc atcgtgtctg atattggcat agtggccggt
    11701 cgcaataaag tcagctccaa gatcatccgc cacctggaag agacctccaa atttcactcg
    11761 ttcattacac cgaacacagg gattaggtgt ggttccttca atataacccc caatgaagtc
    11821 atctatgact cccttctgaa agcgttcgcg ggtatcgatg acctggtgag agatccccaa
    11881 tagttctgcc acatgcctag ccattcccac cttacagcat cctcgctcct cccaacgttt
    11941 ggaaacggag gactcatcct cttcctccca gaccttcaat gtgaccccga taacttcgtg
    12001 gccctgctgc tttaatagtt tggctgcaac ggagctatcc acgcctccgc tcattcccaa
    12061 gacaaccctt ttggaaactg agaatggcat tagcgactca acggctctgt ttctaatttt
    12121 ttttctattt tttgctctac cttgggaatc gtcttgccct gtccattcgg gataccttta
    12181 ttgtcgatag gtgcctctaa tttcggagcc gaacccatat cgcactcgcc ttggaaatac
    12241 acgccttctt ccatggcaaa aataggagca tgaacatcac caatgagaac cgccggcttc
    12301 agaagttgga tttttgattt tgcggtgatg acaccctcaa tccggccact gttcacaatg
    12361 gtcccgcctg tgatggtccc tttgataacg gcactttcac ctatgatgag ggtatcatca
    12421 atcgtgattt caccttcaaa gtttccatct acccgcactg tgccctcaaa ttgagcttta
    12481 cctttaaagt caacaccctt acccaaaaat gtataaaatt cctggccaaa gcccaagtcc
    12541 tttttcttgt ctttttcacc ccacatacat cgtttctcct ctcatccagt aaagagccat
    12601 caggtagcag gctgggatct ccatgaccac ctggaaacca gccgcctatt ttttgcagaa
    12661 gactgcctgg ctttatttca ccttcaccgc ttcttctacc gaaattacaa gtttggtctc
    12721 tttgcctttt cgctcatttt tttggggcat attgcaggtg ccattaaaca aaaccccctc
    12781 atccatcgac aaaagagggg cagtaatttt acctacaaaa actgctgggg ctaacaattt
    12841 aactcgatgc ttggcctcaa catcccccgt caattgtccc tgacacataa tggttccagc
    12901 atgaattttg gcagcgatga ccgctttttg cccaataatt aaattaccat cggtatatat
    12961 ttcaccctcc aggcggccat ccacccgaac cgttccctga taccgaatgg ttcccttgaa
    13021 ggtcacctct tcaccaacaa aggcaatgat atcacctgat tcaggctggc tgtctgagac
    13081 caccggaccg tcaaccacag ctggttcctt ctgctcacct tgactcattt ctgccgcatc
    13141 ctgaccgttt tcttcctttt tgttgaaaaa catgagcccc tccctcctcg ataactgtgc
    13201 gctccaattc cttcatacct ggctaggtac ccattaaaaa taactccacc attttccatc
    13261 agcatgatcc ttcaccccta tcaggagagg ccttctgaca cagaagccat tccgctagta
    13321 cattcaaatt ctattccaaa accagtgaag taatccgatc cagctcatca tcggaaaaat
    13381 aatggatgat gacctgtcct cccctgtttc cggacttcac ctgcacttta gttcccaatc
    13441 gttttcgaaa ttgctcctcc aggttacgaa atctattggg ttcctctttc ttcgatttgg
    13501 cgaatttgcc ttttttgtgc acggcaatca tctgctcaat ttgccgaacc gagagttgct
    13561 cggaaacaat tttgcgggca agctggagtt gtgctgccgg agttggaaga cctgccaaga
    13621 ccttcgcgtg gcctaaactg atggcccccg tctcaatata ctcctgaaca tctttaggca
    13681 gcgagagcag cctgataata ttcgcgatgg aggagcgatc ctttcctacg gagcgggaaa
    13741 ccagatcctg agtcaagcca aattccccca ttagccgtga ataggctttg gcttcctcca
    13801 taggattgag attctcacgc tggatatttt ctaccaacgc gatcgctaaa gccttctcgt
    13861 cgttggaatt tctcaccagg acaggaatgg tagacaatcc ggcaattttc gccgctcgaa
    13921 accggcgttc accagcaatc agttcgtaca tgccatcgcc ttttctcctg acgacaattg
    13981 gctgcagtac gccatgctct ttgacggatt gggctaattc atctaattct gcctcaaaaa
    14041 atgtggttcg gggttggtac cggttcggga gaatctggtc aagtggaacc atggagactg
    14101 cctgaccatc ctgctccact ttccagacaa gagtcttttt ttctggaagc agggcctgaa
    14161 gccctttacc taatgctttc ttttccattc ctcaaaatct ccttggcgag gtcaagatat
    14221 gccttagctc cactagacgc ggcattatag gacagaaccg gcttcccatg gctgggcgct
    14281 tcagccaacg ccacattcct tgggataaca gtctgaaaga ctttatctgc aaaaaaccct
    14341 cgaatttcat cttgcacctg ccgcgaaaga ttaattcttg aatcatacat cgtcaacaca
    14401 atgccttgaa tctccagatt cgtgttaaat gactcccgaa tcctttcgac atttcccaat
    14461 aaccgaccta atccttccat tgcataatac tcgcactgca ccggaataat caggccagat
    14521 gctgcgacaa gagcattaat cgtcaagagt ccgaaggcgg gcggacaatc gatgataata
    14581 aaatcataat ccagaattcc gtccaacaca cttttcagaa tagtttgccg atcagggaga
    14641 tttgttagtt cgatttccgc tccgaccaaa tcgccctttg aggctacgac actcagtccg
    14701 cttatgtcag taggtaatat taccttctca atcaaacgtc tttccattaa acattcataa
    14761 attgtcacgc cttgaggctc aatggacaac ccactggtcg cattggcctg tgggtccaaa
    14821 tcaatcaata ggacagattt tccagatatg gccaaagccg cgcaaaggtt gatggacgta
    14881 gtagtttttc caactccgcc cttctgattg gcaatggcaa tggtctgttt caagataatt
    14941 ccataaaaat tcttatgaat agttccacgt ggaacatggc aatggccaga ttatggcgca
    15001 aattttaaaa tcgacaagac acgacttcca tactcaaacg gcaattcata ggagaattct
    15061 tcaaacactt ccatgccatc gagccgagaa ttattgtcaa tacttttgga tcgaaataca
    15121 actagtttcc cggttttttt taaaatgtgc tcaagatacg gcaaaatatg gtctacattg
    15181 acccctttac atatggcgaa atcacacttt tcatcatcca ccaccataga atcaaagtct
    15241 tccaaccgaa cttggagaac ggaagtcttt ggcaactcaa gttttccaat aatataatgg
    15301 aggaaggccg tcttattgga tctgggctcc attaacaaaa catcaagagc aggcaaagcc
    15361 agcttgagcg gtattcccgg aaaccctcct cctgtcccaa tatcaacaat ctttgcatca
    15421 gctttgtttc ccagcgccaa atgtccagcc aaggaatcaa caaatagcaa aatagtccgc
    15481 tccctgtccg tacgaagacc ggtcagatta atagaccggt cccaacgaca caattcatca
    15541 taatacaaac caaatttacc aagtgttgcc tcggtgaaag aaagcccaag cttctgagcc
    15601 ccgcgcttga aatgatctat gaaattaaat gattgatcgc tttgttccac gtggaacatc
    15661 cctacttata tgaatcttaa agtgagaaat tgttttgggg cttaatgaat atggaatttg
    15721 gcaaaaaaca gatttctgct gatactgacg aatgaatata ccgaactagc tggccttttt
    15781 atgtttttca accgcaacaa ggagaagtga aatggcggca ggagtcattc cagaaattcg
    15841 tgaggcctga ccaatggatg aagggcgaac tttttttagt ttttcaacaa cttcatttga
    15901 aaatcctttg atatcatcat aattaaagtc atccgggaca tgacgatgct ctagtttttt
    15961 aaattgccct ataagctgtt cctgccgctt aatatatccc tcatatttga tcgttatttc
    16021 aacagcctca actacgtcag gatcatcctc ttttgttcca tttaagaatt gagccaaatg
    16081 atgatatgac acctcatgcc ttcgaagcag ttgagcaagt gacgagtttg gtgcaaaact
    16141 ttccattgga atgatgtcat catgaaccaa acctgatgac ttaggagccg tggcatgaag
    16201 tctgtttatc tcacgttcaa tctttccctt tttttcttga aatgtatcaa acgtaccctg
    16261 agagacaagc ccgatgcgat aaccttcctc cattaaacga aagtcggcgt tatcctctct
    16321 gagtagaagc cgatattcag ctcttgaagt gaacattcta tagggctcac gtgcatcttt
    16381 tgtgatcaaa tcatcaatca atacgccaat ataggcctga gacctatcta aaattagtgg
    16441 tctgtcattc ttcacctgaa gcgcagcgtt aataccagcc atgattccct g
//
LOCUS       DAORAQ010000052        16483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_605_length_16483_cov_64.122535, whole genome shotgun sequence.
ACCESSION   DAORAQ010000052 DAORAQ010000000
VERSION     DAORAQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16483
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_16483_cov_64.122535"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="PKM72_06730"
     CDS             complement(<1..1694)
                     /locus_tag="PKM72_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60517.1"
                     /translation="MFHVKSRKAITTLSIGLLVGGTSFLSGGTLLAEEIQDKVIHSTG
                     MVHQESPGWAERLKGQTIMEDTQEGRPNRAAMVERQHDRIMLRMENESGAQHADHTAT
                     GFYNTMTNMHQYGAGGQDLLLASDRNVEPVAASGGNCPSTAPVREYDISAINAEITLN
                     QWLDYYPGYMYVLTENIDKVREEETKNAEAREVDGHGDPGAVTNGLQNQWIQPLVIRG
                     NQGDCVKFTLRNQLEFGEEASLHIHGSNMVISKTGQPATTTNRDTVAEEGQVVEMEWY
                     IHPDTQEGGRQFHTFSNDRELTVMGLMGTFVVEPHGSEYLDPLGNGDPTPMASGWQAI
                     IKNGTGPDFREFVLIYHEVGDEAFRPVNKHGDFLPQRDPLTDAYRPGARALNYRSEPF
                     GINNMHVQHEYFGFEDESMGYSSYTFGDAPTTIPRSYLGDPAKFRLVHGGSEVFHSHH
                     PHGGTIRWPRSPRAIDEMPLWHAAKNGPVKYPVIRTKTDRVDVEVIGPSEALDLETEC
                     GSGLCQQLAGDFLFHCHVAHHYVAGMWGYWRVYNTMQTDDNRTDVMPNLRELPDRKG"
     gene            complement(1973..2827)
                     /gene="dat"
                     /locus_tag="PKM72_06735"
     CDS             complement(1973..2827)
                     /gene="dat"
                     /locus_tag="PKM72_06735"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HNP60518.1"
                     /translation="MPDIGYLNGHFSPLGEVKISPDDRGFLFGDGVYEVIRAYHGVPA
                     FLGEHFKRLVRSANEIQLPFSLEQAEFQRLLLHGLQQSGYQEGKIYIQVTRGVAPREH
                     TFPPIGEPTVFLAFRKMVALPTEVCKRGVNVITLPDTRWHRCDIKSLNLLPNVLAKQK
                     AREANAFEGIFVRDGQVTEGATSNIFAVKNGTIITPERNHLVLAGVTQQQVVTLAQAK
                     GLNVQFRPITISEFLQSDEAFLVGTTIEILPVIQVNGETLRDGKPGPITTILQQQFTA
                     HVSRLTMS"
     gene            complement(2841..3155)
                     /locus_tag="PKM72_06740"
     CDS             complement(2841..3155)
                     /locus_tag="PKM72_06740"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNP60519.1"
                     /translation="MNIEQVQTKTIQVFKSATFMVHLWEDRTRGEQWVPSYDPKALGL
                     VGDEFLRTVSNNAVDSGRRSFEFQALEPGTHHLEFSKRMAWKFTAEDRRIFKVIVLQS
                     PS"
     gene            complement(3205..3600)
                     /locus_tag="PKM72_06745"
     CDS             complement(3205..3600)
                     /locus_tag="PKM72_06745"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNP60520.1"
                     /translation="MMNDSSSERDMDCRIHEPLSPKEGFVFVGKLEDIPKGKGRVFKV
                     GGKSVAVFRIDDRCFAINDICPHQGASLGKGRLKGFVVSCPWHHQQFDIRSGFGPDGG
                     GYCVVNYDVVVDKGNVFVCLKKRDWLTGE"
     gene            complement(3611..4108)
                     /locus_tag="PKM72_06750"
     CDS             complement(3611..4108)
                     /locus_tag="PKM72_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNP60521.1"
                     /translation="MLSFLKGKGAPSKKRIIFLLVMALFLISGALLFNAPEQTQVINI
                     NFPEGGVLKAEVADTPEKLLFGLAFRNALPPNEGMIYIFEDSGLHRVWTKEFQFPVDV
                     IWVDESKIVVHIVEGALPCRERQCPWYGPPPQDARYIVEANAGFVDQAKIKIGAQVTF
                     TLLVS"
     gene            4337..4669
                     /locus_tag="PKM72_06755"
     CDS             4337..4669
                     /locus_tag="PKM72_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60522.1"
                     /translation="MRYLPTLLLGISLVFISFTSTSAHPSISTAIDRFVSTLYPKGSH
                     YFWVINNTTTESPEEIIVDLNTSVRNLPDEAPTESRFLLLVVEGELFAAQKIPLNANV
                     DCRSDEEV"
     gene            complement(4757..5254)
                     /locus_tag="PKM72_06760"
     CDS             complement(4757..5254)
                     /locus_tag="PKM72_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60523.1"
                     /translation="MHRVLLSVLLSVVFVVAGSSAAFALQSGGVEIGDLETGSVVGQP
                     FQKLDVDIELNAMEIGGKKVWYPPVSILSLISSATGGRAGRPVLVKVTNTMKEAHGFS
                     LSAASTQTGPTTMEINLELAPGETKYIGIPISDLTYVTSNSLLNYKCQLHSAHLGGQL
                     LVLTK"
     gene            complement(5636..6274)
                     /locus_tag="PKM72_06765"
     CDS             complement(5636..6274)
                     /locus_tag="PKM72_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP60524.1"
                     /translation="MDQFIRITFPVPPLSCNCSIIGDNETKQAIVVDPGGNPERILGE
                     IDAMGFTVTAVLHTHAHFDHFLASGHIKEATGASLFLHPQDQDLWGMLEIQCRMFNIP
                     YVPVPPPDKWIKDESEVQVGPYTGTVIHTPGHTPGSVCFFFESQSLVFSGDTLFRGGI
                     GRTDLWGGDGQIIERSIRERLYTLKESTLVIPGHGPESSIGWEREHNMFVHG"
     gene            6565..7332
                     /locus_tag="PKM72_06770"
     CDS             6565..7332
                     /locus_tag="PKM72_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60525.1"
                     /translation="MSKAFYLLAAILVLFLTGCPEDHSAFQEENKKLTKKIDKLESFI
                     QSLQEGNKVLQQQIDRLNQDARTAEATHKEQLRDAQAQMAELANSPKKDGAKIKILEE
                     EHRKLEGENKWLRSQRDQMRKTLTLHQIGGQSQELPFPFSSASEIIEGALTKNGYSIL
                     SSMQTDQKAVYITDRKTSLPPSLELSGFRNQYLLSIEKGPSDHTIIWVRAEFEKISKN
                     GQMFSAQQSEVTDIELRLIQEIHQALSTGAAAQAKNF"
     gene            complement(7420..8913)
                     /locus_tag="PKM72_06775"
     CDS             complement(7420..8913)
                     /locus_tag="PKM72_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNP60526.1"
                     /translation="MRYTDLSRGEAIYEDFLRRAGRIPFLGLGLSVDVYSPDIFDLYE
                     ELRSQQIPMAYLEIFHAASEALAMVRARLSDIPLAYHAEGIWVTQPDWDTAYNSQERL
                     QATAFNLRMLQPHWVNQECAAKEIGGFSFGTYLPPVFTRASAEITAYQTWNAQLQLDQ
                     FDWGQQVSSPLLLLEVPPLTYFSMGEMPYAEFFANISAKSPCGLVLDLGHVWTVYRYS
                     GAWRKQGLESFFEAFLEQFPLERVIEIHIAGLDCHPNIHTGLDAEPHGPQNWIDAHEA
                     PIPDELLMLLTRVLGDSRLLNLKGIALEVDNKPIPLICREMKMVMEMVEPPGHLIAQV
                     RSSATELQRELNFPVENVEPSRETRDGLTLQYREYMALVREVLSGRLDVPGSLDAEMN
                     MGLHVYATQYLPYEILTWGGDVRVMFPNACDLLAQYGISLKQFVEFWFAHPRTAESGI
                     EYDFFLLKIDLFVKFIGQKFPEASSLVQQEAELLIQGYLLASQGAST"
     gene            complement(9181..10611)
                     /locus_tag="PKM72_06780"
     CDS             complement(9181..10611)
                     /locus_tag="PKM72_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNP60527.1"
                     /translation="MADPRGFLKYKREGPQRRPVELRVLDWKEIYEPFSDDKLKTQGA
                     RCMDCGVPFCQSPSGCPVVNLIPEWNDLVHKGRWKDALKALHATNNFPEFTGRLCPAP
                     CESACVLGINSDPVSIRVIEWNIIDKGFEEGWVEPVLPVVSSGKRVAIVGSGPAGLAA
                     AQQLARTGHEVTVLEKADRIGGLLRYGIPDFKMEKWVLDRRLEQMRKEGVNFQTSVCV
                     GVDTTVEALRRDYDAVLLAMGAEQPRELPVPGRDLKGVHLAMDYLTQQNKQVAGDTLL
                     GEHISAKGKRVVIIGGGDTGSDCLGTAHRQGCVEAHQFELLPEPPPNRAQSTPWPLWP
                     MQLRTSHAHEEGCDREWSISTTKFSGENGHLTRLHATRVNFENGKVVPVSGAEIKMDV
                     ELVLLAMGFVGPVKGGLLDSLGLQYDSRGNVAVDEHFMTNMDGVFATGDTKRGASLIV
                     WAIAEGRKSAAGIHRYLQAGRSFRVS"
     gene            complement(10604..15175)
                     /gene="gltB"
                     /locus_tag="PKM72_06785"
     CDS             complement(10604..15175)
                     /gene="gltB"
                     /locus_tag="PKM72_06785"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNP60528.1"
                     /translation="MHNQPVWNLPGLPSAQGLYHPGNEHDGCGIGFVAHIKGQKSHDI
                     IEKGLEVNKNLTHRGAQGSDPCTGDGAGILSQIPHDFFHRVAAESGIDLPVAGAYGVG
                     MVFLPQDAKTRQQCEAVCEAIIGEEGQRFLGWRDVPAKEDQIGEQARKTMPAIRQFFV
                     ARELLNPMQFERKLYVIRKRITRAVRESALPGKEWVYISSLSANTIVYKGMLLPEQVS
                     LFYPDLADPTFTSALALVHSRFSTNTFPTWALAHPYRYSVHNGEINTLKGNVNWMRAR
                     QGRLASELFGEDLKKLFPIIDDSTQSDSACLDNAIEFLVMAGRSLPHAMMMLIPEPWV
                     GNPQMDFDRRGFYEYHAAVMEPWDGPAAVCFTDGKLVGATLDRNGLRPCRYQITKDDV
                     VVLASEAGVLPVDPKTIRMKGRLQPGRMFVVDTVQGRILDDEEIKADITKRKPYRQWL
                     TQYRVSLDELPEPLNVPQPDHPTLRQRQQAFGYTVEELKMVLIPMAVTGEEPISSMGT
                     DTPLAVLSERPQLLFKYFKQLFAQVTNPPIDPIREHLVMSLFTNIGPKPNVIAEIPEA
                     CRRIKLQQPILSNVDLQKIRMIGDPHFKSKTLSLLFNVAEGPEGMAAALDQLCQQASK
                     AIKDGEKFLILSDRGINAEWAPIPSLLGVSAVHHHLVREETRTEVGLILETGEPRDVH
                     HFACLIGYGVGAINPYLVFESLVDMEREGYFPESVDAATAESKFIKAVNKGLLKIFSK
                     MGISTVQSYCGAQIFEAIGLNNQLIDRFFTGTASRIEGIGLSEVGEETLRRHALAYRP
                     VPLRQLDFGGEIHYRIQGEHHNWNPETIYKLQHASRANDAKAYADFSALVNKEDQRRS
                     NLRGLFEFNWAAEPIPLEDVEPASEIVKRFTTGAMSFGAISKEAHETLAIAMNRIGAK
                     SNTGEGGEDPTRFEPLPNGDSKNSYIKQVASGRFGVTAHYLVNARELQIKMAQGAKPG
                     EGGQLPGHKVDEIIARLRYSTPGVQLISPPPHHDIYSIEDLKQLIFDLKNSNPEAAIS
                     VKLVAEVGVGTVAAGVAKAHADKVLISGDSGGTGASPLSSIKYAGVPWELGLAETHQT
                     LVLNDLRGRIRVETDGQLKTGRDVVIATLLGAEEFGFSTAPLIVEGCIMMRKCHLNTC
                     PVGVATQDGELRKQFTGKPEHVVNFFMFVAEEIRSLMAKLGFRTIREMIGRVDKLKVH
                     KAVDHWKAKGLDLSPLLIKPDVGPEVATSCVQKQDHGLKEILDNQLVEWCQPAIERGE
                     SVSLDLPIRNVNRTTGTVLSSHIARRYGPDGLPSDTIRIKFTGSAGQSFGAFLSKGIT
                     LILEGESNDYLGKGLSGGKIIVIPPKGVTYSPEDTILIGNTSLYGATGGEGYFYGIAG
                     ERFAVRNSGARAVIDGTGDHGCEYMTGGVVVVLGKTGRNFAAGMSGGDAYVLDEDGQF
                     PARCNMGMVELEPVVSSEDKQTLRGMIEAHLRYTQSANARRVLESWDMMLPKFVKVMP
                     SDYKRVLQERKTALAKEHAQREREAVSRG"
     gene            15483..>16483
                     /locus_tag="PKM72_06790"
     CDS             15483..>16483
                     /locus_tag="PKM72_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNP60529.1"
                     /translation="MHQEPALRDLESMEDVWEAFRPDLIEVESQITKHLESHAPLINT
                     VAGHIFASGGKRIRPLLLILCARLCGYLQKDFLLLGSLVEFIHTATLLHDDVLDEADL
                     RRGQPTARKLWGNQASILVGDYLYSQAMALIATFHNHGINEALADACRKMAEGEVLQL
                     CANSQPHLSEAAYLKIVEYKTAALVAASCKVGAIVGGASIEEQAALYRFGYHLGMAFQ
                     LADDRLDYAADRAKLGKALGQDIRQGMITIPLLHLLQTCSSQDNQWITEKILAHAIED
                     EDVTKIIQLMQKQGSLAYSTARAREFVEAAALDLEPFSACTAKRSLSITACYMVNRD"
BASE COUNT         4463 a         4435 c         3485 g         4100 t
ORIGIN      
        1 ccttttctat cgggcaactc tctgaggttt ggcatgacat ccgtgcggtt gtcatctgtc
       61 tgcatggtat tatatacccg ccaatacccc cacattcctg caacgtaatg atgggcgaca
      121 tggcaatgaa atagaaaatc ccctgccagt tgttggcata agccagaccc acattccgtc
      181 tccaaatcca aagcttctga aggcccgatt acttcaacat caaccctgtc ggttttagtc
      241 ctgattacgg gatatttgac cggaccgttt ttcgcggcat gccacagtgg catttcgtca
      301 attgcccgcg ggctcctcgg ccatcggatc gtcccaccat gtggatggtg ggaatgaaat
      361 acctcagacc ctccgtgaac caacctgaac ttggcaggat caccgagata ggatcgagga
      421 atcgtcgttg gagcatcacc aaaggtgtaa gaactatagc ccatcgattc atcttcaaaa
      481 ccgaaatatt cgtgctgcac atgcatgttg ttgatgccaa atggctcact ccgataatta
      541 agagctcgag ccccaggacg ataggcatcg gtgaggggat cccgttgtgg taagaaatca
      601 ccatgcttgt ttaccggacg aaacgcttca tcacccacct catggtaaat caacacgaac
      661 tcacgaaaat ccggtcccgt tccatttttg ataatagctt gccatccact ggccataggt
      721 gttgggtccc cgtttcctaa cggatccaaa tattcggaac cgtgtggttc aaccacaaat
      781 gtccccatga gacccataac cgttaattcg cgatcattac taaatgtatg aaattgtcgg
      841 ccaccctcct gggtgtctgg atggatatac cattccatct ctacaacttg cccttcttca
      901 gccacggtat cacgattggt tgtcgtagca ggctgtccgg ttttgctgat aaccatattc
      961 gagccatgaa tgtggagact ggcctcctca ccaaactcga gttgattccg gagagtgaat
     1021 ttcacacaat cgccttgatt tcctctgatc acaagaggtt gaatccattg gttctgaaga
     1081 ccgtttgtca ccgcgccggg gtcaccatga ccatccacct ctctcgcttc cgcattcttg
     1141 gtttcctctt cgcgtacctt atcgatattc tccgtcagga catacatgta gcccggatag
     1201 taatcaagcc attggttgag ggtaatctcg gcattgatgg cggaaatgtc atattccctg
     1261 acaggggcag ttgatggaca atttccacca ctcgccgcaa caggttccac attccgatcg
     1321 gatgccaaaa gtaaatcctg gcctcctgcg ccatattgat gcatgttggt catcgtatta
     1381 taaaaaccgg tcgctgtatg gtcggcatgt tgggcaccgg attcgttttc cattctcaac
     1441 atgatccgat catgttggcg ttctaccatg gcggcccggt tcggacgacc ttcctgggtg
     1501 tcctccatga ttgtctgccc ctttaaccgc tctgcccatc caggtgattc ttgatgaacc
     1561 attcccgtgc tgtggataac tttatcctgg atttcttcgg ccaggagcgt tccccctgac
     1621 aaaaaacttg ttcccccgac taacaatcca atggaaagtg tggtgatggc tttcctagat
     1681 ttcacgtgaa acatggaccg gcctccttaa tgtgaaataa actgacaaac caaagacttt
     1741 tcttacaaat caaacagtcg ataatattcc atcaaaatgt tgggaaaggc caaaggtaat
     1801 ccaatcgatt caggctgtca agtccgaaag atttaagttt gatttcattc gcatttatca
     1861 tcagaaatat taaaatggca tacccgaaat gaaaatgtaa atctaggcaa acggtcgccg
     1921 aatggctgcc agtttaattc cccttcagat taaaaattct gacttttaga aactacgaca
     1981 tcgtgaggcg ggaaacatgg gcagtaaact gctgttgcag aattgtggta atgggaccag
     2041 gctttccatc ccgtagcgtt tccccattaa cttgaatgac tggcagaatt tcaattgtgg
     2101 tacccacaag aaaggcttcg tcggattgca ggaactctga aatagtaata ggtcgaaatt
     2161 ggacgtttaa accttttgcc tgagctaagg tcaccacctg ctgttgcgtc acaccagcga
     2221 gaaccaaatg attgcgttcc ggagtgataa ttgtgccatt tttcacagca aagatattac
     2281 tggtagcccc ttcggtcacc tgtccgtctc tcacaaaaat tccctcaaag gcattggcct
     2341 cccgggcctt ctgcttagcc aatacattcg gaagcaaatt caggcttttt atatcacagc
     2401 ggtgccaacg tgtatcaggt agggtaatga cattgactcc ccgtttgcag acttctgtcg
     2461 gcaaggcaac cattttccga aatgccaaaa agaccgttgg ttctccaatt ggggggaagg
     2521 tatgctcccg tggagccacc cctctggtca cctgaatata gattttccct tcctgatacc
     2581 cactctgctg aaggccgtga agtaggagac gctgaaattc ggcttgttct aaagaaaaag
     2641 ggagctggat ttcgttggca ctccgaacga gcctcttgaa atgctcaccc aaaaaggcgg
     2701 gaaccccatg ataagcacga ataacctcat agacaccatc cccaaataag aacccacgat
     2761 catccggaga gatcttcacc tctcctaatg gagaaaagtg tccattgaga taccctatat
     2821 ccggcatgat gaaaatttat tcaggaaggt gactgcagga caatgacctt aaaaatccgt
     2881 cgatcctctg ctgtaaactt ccaagccatc cttttactaa attccaaatg atgtgtccct
     2941 ggttccaacg cttgaaattc aaatgatcgc cgaccggaat ctaccgcatt attactaaca
     3001 gttcgaagaa attcatctcc caccaatccc agcgctttag ggtcataact tggcacccat
     3061 tgttccccgc gggtccgatc ttcccataaa tgcaccataa aggtggccga tttgaacacc
     3121 tgaatagttt ttgtctgcac ctgctctatg ttcatgcttt caccttgacg aaaccttgac
     3181 tccttgttac gcctgtaaaa tctatcattc tcctgtcagc caatcacgtt ttttcagaca
     3241 gacaaaaaca tttcccttat caaccaccac atcataattg accacacaat aacctccgcc
     3301 atctggcccg aatcccgatc gaatgtcaaa ttgttgatga tgccacgggc aagaaaccac
     3361 aaaacctttc aatcggcctt tccccaatga ggctccctgg tgcggacaaa tatcattaat
     3421 agcaaagcac cgatcatcaa tgcgaaatac agccacactt ttcccgccga ccttgaatac
     3481 acgccctttt cctttgggga tatcctccag cttcccgaca aacacaaatc cctctttggg
     3541 actcaatggt tcatgtatcc tgcagtccat atcacgctcg gaactggaat cattcatcat
     3601 ccgtttatct ttacgagaca agcaatgtaa aggttacctg cgcacctatc ttaattttgg
     3661 cttggtccac aaacccagca ttggcttcaa caatataacg cgcatcttga ggcgggggac
     3721 cataccaggg acattgcctt tcacggcatg gaagagcccc ttcgactatg tgcacgacaa
     3781 ttttactctc atcgacccaa atcacatcaa caggaaattg aaactctttt gtccagaccc
     3841 gatggagacc ggaatcttca aaaatataaa tcattccctc attgggagga agagcatttc
     3901 taaatgccaa tccgaataaa agtttttccg gtgtatccgc aacctccgct ttcaatacac
     3961 caccctcggg aaaatttata ttgataacct gtgtctgttc cggagcatta aacaataatg
     4021 cgccggaaat caggaacaac gccattacca gaagaaagat aattcgcttt ttacttggag
     4081 cccctttacc tttgagaaaa gacaacatga ttttacatat ttccttattt ttcttccctt
     4141 gcctcggtcc atggaaattt ccccaggcaa gctcaattca atcatttccc gatttgctca
     4201 aacaacagac gagggataag atattaagat atcatgtaga ttgttaatat ttcagccaca
     4261 atgaaatatg gataaaaagc cctctctcga cattcatcac aaagtaaacc tttacctcat
     4321 accatggagg cctcgtatgc gctacctccc aactctcctg ctcggaatct ctttggtatt
     4381 catttccttt accagcacct cagctcaccc ttcgatttca accgccatcg accggtttgt
     4441 ctctaccctg tacccaaagg gtagccatta cttctgggtg attaataaca ccacaaccga
     4501 atcccccgaa gaaataattg tcgacctcaa caccagcgtc agaaatttac ctgatgaggc
     4561 accgacggag agtcgttttt tattattggt tgtagaagga gaactgtttg cggctcagaa
     4621 aattccgttg aatgctaatg tggattgtcg tagcgatgag gaagtgtagt tgataaaggc
     4681 attcatatat taaataagcc ccatcccttg tgaaaaggat ggggcttatc ttttcagaac
     4741 tacgtctgaa taatttctac ttggtcaaca ctaataattg tccaccaagg tgggctgaat
     4801 gcagttgaca cttataattc aacaaactgt tcgaagtcac ataggtgaga tcactgatgg
     4861 gtatcccaat atacttggtt tcacctggag ccaactcaag gttgatttcc atcgtagttg
     4921 gaccagtctg tgtggaagcc gcggaaagag aaaaaccgtg cgcctccttc atagtattgg
     4981 tgaccttgac caaaaccggc cttccggctc taccacctgt cgcactggaa ataagactca
     5041 aaattgatac cggaggatac cagactttct tgccaccaat ttccatggca ttcaactcaa
     5101 tatccacatc caacttttgg aatggctggc ccacgacaga tcctgtttca aggtcgccaa
     5161 tttccacacc accggactgt aacgcaaatg ctgctgaact tccggccaca acgaacacta
     5221 cacttaaaag gacggataga agcactctgt gcatcgccta cctcctcatt gagaagctgt
     5281 tgataatgaa agttttacct gaaaaacgca agccaatttt cttatttata ccgtcttagt
     5341 tttcaacaag agggttggtt atagcatatg acattcttcg acgcaagatg gtatttataa
     5401 ttgcaattct tggagattga aatatgaaag tgtgccctat taaatcaata agagtactaa
     5461 gaaatactca accctttttc attttttttt aggattaaga ttcctttcct caatgaagaa
     5521 tgagttttta taacggggca attatttttt taagactatt tttctcttat ttctcaacat
     5581 acttaatagc caatcccaaa agtcccatta tctttcaaac attaatcctc aatgcttatc
     5641 catgcacaaa catattatgc tctcgttccc atccaatgga cgattcaggc ccatgccctg
     5701 ggatcacaag cgtgctttct ttgagggtgt acaagcgttc tcgaatagat cgctcgatta
     5761 tctgaccatc tccaccccaa agatcagttc ggccaattcc gccacgaaac aaggtatccc
     5821 ctgaaaacac caaactttgg ctttcaaaga aaaaacaaac cgagccagga gtgtggccag
     5881 gagtatggat cacggttccc gtatagggac caacttgcac ttctgactca tctttaatcc
     5941 atttgtccgg cggagggaca ggcacataag gaatattaaa cattcgacat tgaatttcca
     6001 gcatgcccca aagatcctga tcttgcggat gcaaaaacag ggaagctccg gtcgcctcct
     6061 taatatgacc ggaggctagg aaatggtcaa aatgggcatg cgtatgcaaa acggcagtga
     6121 ccgtgaatcc catggcatca atttccccga gtatgcgctc agggtttccc cctgggtcca
     6181 ccacaatggc ctgtttagtt tcgttatcac cgatgatcga acaattacat gaaagtggag
     6241 ggacggggaa agttattcta ataaactgat ccattccaga ttcctcataa tgtttcaaca
     6301 cgacctacct tccaccctta agatgtcttc agattttcac gatttctcaa aggaataaca
     6361 gattattccc cattctttat accccctcaa tatggaagtt tgaaataaga actgattagg
     6421 aataaaatac ctaataaaag gtacccattt gatcaaatca caacactatc ctccattcac
     6481 ggatgcgctg agccggtgaa aactcaagca ttcttccagc gcccgcgaac atcccggcag
     6541 aggcaagata tactcccctg cagtgtgagc aaagcctttt accttttggc agccatactc
     6601 gtactctttc tcaccgggtg tcccgaagat cattctgctt ttcaagaaga aaataaaaag
     6661 ctgaccaaaa agatcgacaa attggagtca tttattcaat ctctgcagga aggcaataaa
     6721 gtcctacaac aacaaatcga ccggctgaac caagatgcta gaacagccga agcgacacac
     6781 aaagaacaac ttcgggatgc acaggctcaa atggcggaac tggcaaacag ccccaaaaaa
     6841 gatggagcca aaattaaaat attggaagaa gaacatcgaa aattggaagg agaaaacaaa
     6901 tggctcagaa gccaaaggga ccagatgagg aagaccctta cccttcatca aattggtggt
     6961 caaagccagg agttgccgtt tcccttttcc tcagcttcag aaatcataga aggcgcactc
     7021 acaaaaaacg gctattcaat actttccagc atgcaaacag atcaaaaagc cgtctatatt
     7081 actgatcgca aaacgtcact tcctccctca ctggaactat caggttttcg gaaccaatac
     7141 ctcctctcga ttgagaaagg gccttccgat cataccatca tttgggtccg tgccgaattc
     7201 gaaaaaattt ccaaaaacgg ccagatgttc tcagctcagc aatctgaagt caccgatatt
     7261 gaactgcgcc tcattcagga aattcatcaa gccctgtcta ccggagcagc agcccaggcc
     7321 aagaactttt aaatccctgc caagcagaag ttcccaatcc actattcacc tgatcaggtc
     7381 actgacagac agaaccgatt ttcctaggaa ccgtcgtggt tacgtcgatg ccccctgaga
     7441 tgcgaggagg tagccctgaa taagaagttc ggcttcctgt tggacaaggg aacttgcctc
     7501 aggaaacttc tgcccaataa atttcacaaa taaatcaatt ttgagcagaa agaaatcata
     7561 ctcaatccca gactcagctg ttcgaggatg ggcaaaccaa aattccacaa attgtttcaa
     7621 tgaaatcccg tattgggcca aaagatcaca cgcattggga aacatcaccc tcacatctcc
     7681 accccatgtg agaatttcat atggtaaata ctgagtcgca tacacatgaa gccccatatt
     7741 catttcagca tccaaagaac caggtacatc cagccgacca ctcaggactt ctctgaccag
     7801 tgccatgtac tccctgtatt ggagggttaa tccatcacgg gtttccctag aaggttcgac
     7861 gttttccact ggaaagttta attccctttg caattctgtg gcagacgaac gcacctgagc
     7921 aatcaaatgc cccggaggct ctaccatctc catcaccatt ttcatttccc ggcagataag
     7981 cggtatgggc ttattgtcta cttctaaggc gattcctttg agattgagta gacgagaatc
     8041 ccctaaaacc cgagtcagga gcataagtaa ttcgtccggg atgggagcct catgggcatc
     8101 aatccagttt tggggtccgt gtggctctgc atcaagtccg gtgtggatgt tcggatggca
     8161 atccagaccg gcgatatgaa tttcaatgac tctctccagg ggaaattgct caagaaaggc
     8221 ttcgaaaaat gattccagcc cctgttttcg ccaagcgccg gaatatcgat agaccgtcca
     8281 gacgtggccc agatccaata ccaacccgca gggtgacttg gctgaaatgt tcgcgaaaaa
     8341 ctctgcataa ggcatctcac ccatcgaaaa ataggttaag ggcggaacct ccaaaagcag
     8401 caatggcgag ctgacctgct gtccccaatc aaattgatcg agctgcagct gagcattcca
     8461 cgtctggtaa gccgtaattt ctgcagaagc tctggtaaat acgggaggca agtaggtccc
     8521 gaacgaaaag ccgcctattt ctttggccgc acattcttga ttaacccaat gaggctgtaa
     8581 cattcgaaga ttgaaagcag tagcctgtaa tcgctcttga gaattgtacg cggtgtccca
     8641 atcaggttgt gtgacccaaa tcccctcggc atggtaggcc agaggaatgt cagaaagtcg
     8701 ggctcgaacc atcgccaaag cctccgaagc cgcatgaaag atttcaagat aggccatcgg
     8761 gatttgctga cttcgaagtt cctcgtatag gtcaaaaata tcaggtgaat acacatccac
     8821 cgataaacca aggcccagaa atggaatcct gcctgcccgc cgaaggaaat cttcgtaaat
     8881 agcctcccca cgtgacaaat ctgtatagcg caaaggtttc ctcattcagt gacttaagaa
     8941 gctaagggga taataatcca gaggaaagac gttcaaaaaa tgcgcgtcag caagctcaat
     9001 attaccggga cgaacagagc aggggaagga ggcatagtca cagccacgtg acggacggac
     9061 tgctgttggt aggactcaac actttcttga tggtctgtga tggacacaca cccactttac
     9121 atttcacaag agcttttgaa aaaccgacct aagcggaaaa tatccaagaa tggaaagaaa
     9181 tcaggatacc cgaaaggatc gtccagcttg tagataccgg tgaatcccgg ccgcagactt
     9241 tctaccttct gcaatagccc agacaatcaa agacgcgcca cgcttagtgt cccctgtggc
     9301 aaacacccca tccatattag tcatgaaatg ttcatcaaca gccacattcc cacgagaatc
     9361 atattgaagc ccaagagaat ccagcaggcc ccctttaacc ggcccgacaa accccatcgc
     9421 caacaagacc agctcaacat ccatcttgat ctcggccccg gaaacaggaa ccacctttcc
     9481 attctcaaaa ttcacacggg tcgcatgcaa tctggtgagg tgcccatttt cccctgaaaa
     9541 ctttgtggtg gagatactcc attcgcgatc acacccttct tcgtgggcat gcgacgttcg
     9601 taattgcatg ggccataaag gccatggtgt cgattgagcc cgattcggtg gaggttcggg
     9661 taacaactca aattgatgag cttcaacgca cccttgacga tgagccgtac caagacaatc
     9721 cgatcccgta tcaccaccac cgataatcac cacccgtttg cctttagccg atatgtgctc
     9781 tcccagaagg gtatccccgg ccacttgctt attctgttga gtaagataat ccatggcgag
     9841 atgaacgccc ttaagatctc ttcctggaac gggcaattct cttggttgct cggcccccat
     9901 cgcgagcaac accgcatcat agtcacgacg gagtgcttcg acagtggtgt ccacaccaac
     9961 gcacacgcta gtctgaaagt tcaccccctc tttccgcatc tgctccaatc gtcggtctaa
    10021 tacccacttc tccattttaa aatccggaat accatagcga agcaagcctc caatccgatc
    10081 agccttttcc aaaactgtca cttcatgacc agtccgggct aattgttggg ccgccgccaa
    10141 ccctgctgga cccgacccga caatggcaac ccgcttcccg gatgagacaa ccggtagcac
    10201 cggttcgacc catccttctt caaacccttt atcaataata ttccattcaa tgactcggat
    10261 agagaccgga tcgctattaa tgcctaacac acatgctgac tcgcatggag ccggacagag
    10321 acgtccggta aactcaggaa agttattggt cgcatgaagc gctttgaggg catctttcca
    10381 tcgacccttg tgtactagat cgttccattc cgggatcaaa ttcaccacag gacagccaga
    10441 ggggctttga caaaatggca ccccgcaatc catacagcgt gcaccctgcg ttttgagttt
    10501 gtcgtcggaa aagggttcat aaatttcttt ccaatcgaga acccgtagct caaccggccg
    10561 tcgttgaggt ccttcccgtt tatatttcaa aaaaccacga ggatcagcca cggctcaccg
    10621 cctcccgctc ccgttgagca tgttctttgg ccaaggccgt tttcctttcc tgcagcactc
    10681 gcttgtaatc actgggcatg actttgacaa atttcggcaa catcatatcc cacgattcaa
    10741 gaacccttct ggcattggca ctttgggtgt aacgaagatg ggcctctatc atcccacgaa
    10801 gggtctgttt atcttccgat gacacaacag gttctaattc caccatgccc atattgcaac
    10861 gtgcggggaa ttgcccatcc tcatccaaca cataggcatc gcctccggac atcccggccg
    10921 caaaattacg tcccgttttc ccaagaacca ccaccacacc cccggtcata tattcacacc
    10981 catgatcacc ggttccatca atgacggccc gtgcgccact attccggaca gcaaaccgtt
    11041 ctcccgcaat gccataaaaa tacccttcac cacctgtcgc cccatagagg gacgtgttcc
    11101 cgatcagaat cgtgtcttcc ggcgaatacg tcactccctt cggtggaatc acaataattt
    11161 tccctccaga gagacctttc cccaaatagt cattcgactc gccttccaga atgagcgtaa
    11221 tacccttcga caaaaaggca ccaaaggatt gtccagccga tccggtaaac ttaatccgaa
    11281 tcgtgtcaga agggagtcca tccggcccgt acctacgggc aatatggctt gacagaacag
    11341 tcccggtggt ccggttcaca tttcgtatag gcagatccaa ggaaaccgac tctccccgct
    11401 caatggctgg ttgacaccat tccaccaatt gattatccaa aatctccttc agaccgtgat
    11461 cttgtttttg cacacacgat gtggccactt caggaccgac atccggcttg atcaataacg
    11521 gagacaaatc cagccctttg gctttccaat gatcaacagc tttatgcact ttgagcttat
    11581 ccacacgtcc gatcatctcc cgaatggtcc tgaatccgag ctttgccatc agagagcgaa
    11641 tttcttccgc cacaaacata aagaagttca caacatgctc tggtttcccc gtaaattgtt
    11701 ttcgcaactc cccatcttgg gtggccaccc ccacaggaca ggtattgagg tgacattttc
    11761 tcatcatgat gcacccctca acaatcaatg gcgccgtaga aaaaccaaat tcttccgccc
    11821 ccaaaagcgt cgcaatcacc acatcacggc ccgttttaag ttggccgtcg gtttccactc
    11881 gaatacggcc acgcagatca ttgagcacga gcgtctggtg cgtttcggcc agacccaatt
    11941 cccagggtac tcccgcatac ttgatggagg ataagggaga ggccccggtt ccaccggaat
    12001 caccgctgat aaggacttta tcggcatgag cctttgccac tcctgccgcc acggttccga
    12061 ccccgacttc ggccacgagt ttcacggaaa tcgcggcttc gggattggaa ttcttcaggt
    12121 caaaaatgag ttgcttgaga tcctcgatag aataaatatc atgatgagga gggggcgaga
    12181 tcagttgaac tcctggagtg gaataccgaa gcctggcaat aatttcatcc accttgtgtc
    12241 ccggcaactg tcctccttca ccaggcttag ccccctgggc cattttgatc tgcaattccc
    12301 gggcattcac cagataatgt gccgtcaccc caaaacgacc ggacgccacc tgctttatat
    12361 aggaattttt ggaatccccg tttggcaaag gttcaaatcg tgttggatcc tcgccgccct
    12421 ctccggtatt acttttggca ccgatgcgat tcatggcgat ggccagtgtt tcatgcgctt
    12481 ctttgctaat tgccccaaat gacatggcac ccgtggtaaa ccgcttcaca atttcactgg
    12541 caggttccac gtcctcaagg gggatgggtt cggcggccca attaaactca aacaacccac
    12601 gaagattgga gcgccgttgg tcttccttat tcacgagggc tgaaaaatcc gcataagctt
    12661 tggcatcatt cgcacgcgac gcatgctgaa gtttgtaaat cgtttcagga ttccaattat
    12721 gatgctctcc ctgaatccga taatggattt cgccaccaaa atctaattgg cgcaggggga
    12781 cgggccgata ggccagagca tgccggcgca aggtctcttc acccacttca ctgagaccaa
    12841 tgccttcgat ccgtgaagcc gttccggtaa aaaaccgatc aatcaactgg ttattcaggc
    12901 caatggcctc aaaaatttga gcgccacaat aagactggac cgtagaaatc cccatctttg
    12961 agaaaatttt cagcaatcct ttgttaactg ccttgataaa cttggattcg gccgtcgccg
    13021 catcgacact ttcaggaaaa tacccttcac gttccatatc cacgagggac tcaaacacca
    13081 aatacggatt gatggccccg actccgtatc caatgaggca ggcaaaatga tgcacatcac
    13141 gcggttcccc tgtttccaga atcaacccga cctctgttcg ggtttcttct cgaaccagat
    13201 gatgatgaac ggctgagacc cccaataaac tgggaatagg tgcccactcg gcattaatgc
    13261 ctcgatcgct cagaatcaga aacttttccc catccttaat ggctttcgaa gcttgctgac
    13321 ataattgatc aagagctgca gccatgccct caggtccctc agccacgtta aagagcaatg
    13381 acagggtctt acttttaaag tgaggatctc caatcatgcg aatcttttgc aggtcaacat
    13441 tgctaaggat cggttgctgc aatttgatcc ttcggcaggc ctccgggatc tccgcaataa
    13501 cattcggctt aggcccgatg ttggtgaaaa gggacatcac cagatgttca cgaatcggat
    13561 cgataggagg attcgtgact tgagcaaaca attgtttaaa atatttgaaa agaagttggg
    13621 gccgttcgga caacacggct aaaggcgtat cggttcccat tgaagaaata ggctcttcac
    13681 cggttactgc cataggaatc aacaccattt tcaattcttc caccgtatac ccaaaggctt
    13741 gctggcgttg gcgcaacgtc ggatgatcgg gttgcggcac attgagtggc tcaggcaatt
    13801 catctaaaga cacccgatat tgcgttaacc attgtcgata gggcttacgt ttggtgatat
    13861 cggctttaat ttcttcgtcg tcaagaattc gcccttggac ggtatccacg acaaacatcc
    13921 gacccggttg caggcgtccc ttcatgcgaa tggtcttagg atctaccgga agcactcccg
    13981 cttctgaagc caacaccacg acatcatcct tggtaatttg ataccggcag ggacgcagac
    14041 cattccgatc caaggtcgca ccgacaagtt tcccatcggt aaagcacacg gccgccggtc
    14101 catcccacgg ttccatcacg gccgcgtgat attcataaaa ccctcgtcgg tcaaaatcca
    14161 tttgaggatt tcccacccac ggttccggaa tcaacatcat catggcatga ggcagggacc
    14221 ggccggccat caccaaaaac tcaatggcat tatccaaaca ggccgaatcg ctttgcgtgg
    14281 aatcatcgat aatcggaaag agttttttca gatcctctcc aaacagttca gaggccagcc
    14341 gcccctgtcg ggcccgcatc caattgacat ttcccttcag cgtattaatt tcgccattat
    14401 ggaccgaata gcgataggga tgggctaagg cccaggttgg aaacgtgttt gtactaaagc
    14461 gggaatgcac aagggccaac gccgacgtaa atgtgggatc cgcaagatcg ggataaaaca
    14521 gtgaaacctg ttcgggcaac aacattccct tatagacaat cgtgttggcg gacagactgg
    14581 atatatacac ccattctttc cccggaagag ctgattcccg aacggcccgg gttatgcgtt
    14641 tacgaataac atacagtttt cgttcaaact gcatcggatt cagtaattca cgtgcgacaa
    14701 aaaactgtcg aatggcgggc atcgtttttc gagcctgttc cccgatttga tcctctttgg
    14761 ccggaacatc acgccaccct aaaaatcttt gaccctcttc acctatgatc gcttcacaga
    14821 cggcttcgca ctgttgcctg gtcttggcat cctggggcaa aaacaccata cctaccccat
    14881 aggccccagc taccggaagg tcgataccag attcagcggc aacccgatga aaaaaatcat
    14941 ggggtatctg agagagaatg cctgccccat cgccggtaca tgggtcagac ccttgtgctc
    15001 cccgatgagt cagatttttg ttgacctcca ggcccttttc tatgatgtcg tgtgattttt
    15061 gcccctttat atgggcaaca aacccgattc cacagccatc atgctcattt ccagggtggt
    15121 agaggccttg ggctgaaggc aatcccggga gattccacac tggttggtta tgcacctgtt
    15181 tcacgtttca cctaaaaaat gttgttggcc atcgtcacga gggagacacg ccacccctta
    15241 gcagagactc aatgggccat gtatgccttt gtgtggtccc ggagagcgac ggaagaataa
    15301 gggaagaacg aatcaaaatt aaggaacaac attaatggat ggtcaaaaaa tctgtcaaga
    15361 caatcccctt ttccctatgg aaatttttcc atacagccat ttggttttta aaaatatgtt
    15421 gatcgatttc cagcatgtgg ttcagcttga caatccacag gctaccccct agtatcgccg
    15481 ccatgcatca ggaaccagcc ctcagagacc tggaatccat ggaggatgtg tgggaagcct
    15541 ttcgacccga cttaattgag gttgaaagcc agattaccaa acaccttgaa tcgcatgccc
    15601 cgctcatcaa taccgtcgct ggtcacatct ttgccagtgg agggaaacgt atccgtccgc
    15661 tgcttctgat tctctgcgct cgtctctgtg gatatctcca aaaagacttt ttgttgctgg
    15721 gcagtctcgt cgagtttatt catacggcca cgttattaca tgacgacgtc ttagacgaag
    15781 ccgacctccg acggggacaa cctaccgcaa ggaaactctg gggaaaccag gccagcatcc
    15841 ttgtgggcga ttatttgtat tctcaggcca tggccctgat tgccaccttc cacaatcatg
    15901 gcatcaacga agccttagcg gatgcctgcc gaaagatggc tgaaggggag gttcttcaac
    15961 tctgcgccaa tagtcaacca cacctatcgg aggccgccta cctcaaaatt gttgaatata
    16021 aaacagcagc tcttgttgct gcctcttgta aggttggggc aatcgtggga ggtgcctcaa
    16081 tcgaagaaca agccgctctt tatcggtttg gctatcatct tggcatggcc tttcaattgg
    16141 ctgatgatcg cttggattat gcggcagacc gtgcaaaact cggtaaagcc cttgggcagg
    16201 atattcgcca gggcatgatt accatcccat tactccacct tctccagacc tgctcttctc
    16261 aagacaacca atggatcacc gaaaagattt tggcacatgc cattgaggat gaggacgtca
    16321 cgaaaatcat ccaattaatg cagaaacaag gatcactggc ctattccacc gctcgagcac
    16381 gggagttcgt cgaagccgca gcccttgacc ttgaaccatt ctccgcctgc accgccaaac
    16441 gatccctctc catcaccgcc tgctacatgg tcaaccgcga cca
//
LOCUS       DAORAQ010000053        16358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_609_length_16358_cov_70.793106, whole genome shotgun sequence.
ACCESSION   DAORAQ010000053 DAORAQ010000000
VERSION     DAORAQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_16358_cov_70.793106"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..579
                     /locus_tag="PKM72_06795"
     CDS             280..579
                     /locus_tag="PKM72_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNP60530.1"
                     /translation="MKFVILGKDGPEGSQKRPLYRDAHLKRLEAWAQQGKIILAGPLT
                     DKMGSLIVVESESLEEVQEFAKNDPYTLFGVFQEVTVHPFLQVFPTANHSPKPGK"
     gene            complement(620..1108)
                     /locus_tag="PKM72_06800"
     CDS             complement(620..1108)
                     /locus_tag="PKM72_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNP60531.1"
                     /translation="MMSQYWLFKSEPTTFSIDDLVKSPRQTTCWEGVRNYQARNFLKS
                     MNVGDLGFFYHSNTDPPAIVGIIEVVKAAYPDSFAFDSRSRYFDPRSTLESPRWFLVD
                     VRFVKKFPQALPLDQLRAIKGLGNMELLRKGSRLSVQPVRPDEWQRVLDFVSKKTEPT
                     KR"
     gene            1265..1705
                     /locus_tag="PKM72_06805"
     CDS             1265..1705
                     /locus_tag="PKM72_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HNP60532.1"
                     /translation="MIDQIIQYAETWVPREIWIGMITFSFIAFVGTLIAIPAILIRLP
                     PDYFDNHGHRKWFANHHPIIRTLGLMVKNGLGLIFLVAGIAMLVLPGQGILTMVLGIL
                     FLDFPGKHRVEQKLIRQPQILNAINSLRKKAGKLPFVFHEKSPT"
     gene            1779..3728
                     /locus_tag="PKM72_06810"
     CDS             1779..3728
                     /locus_tag="PKM72_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNP60533.1"
                     /translation="MSLWNKIGWLGIAGLCAVGFGHVVGIINPHEKVNGLWLVVAASC
                     FYVLAYRFYGRFLAQRVMNLDDRRRTPAHRLEDGTNFYPANKYILFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWIVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHAELGPIT
                     GLATAIAVLFIVVVALAGLGLAVVNALYHNAWGTFTIIMTIPIAVFMGLYLQKFRQGQ
                     VGEMTLIGLGLLVLAIIVGRTVAQSDWAEWFLWDRTTLTWSLAAYGFLASVLPVWILL
                     VPRDYLSTFMKLGVIALLAIGVIVLAPTIEMPRTTIFVAGNGPIIPGTLFPFLFITIA
                     CGAISGFHSLVSSGTTPKLISLESQAVVGYGAMLLESFVGVIALIAACLLIPGDYLAI
                     NTNLSAETLETMGFPTAHIKELSTLVEVDVSGRPGGAVSLAVGMASIFSSLPGMSGLM
                     AYWYQFALLFEALFILTTIDAGTRVARYLVQELGGRVYAPLKQINWWPGVLGASLFVV
                     GAWGYLIGTGSIATIWPMFGAANQLLGMLALCIATTVLIKMNKTSYLWVTVIPMVFVG
                     VITLAGCYELLVIFISRALSADGSQSLTMSINASLVGLVAILALIVLTDSARKWYGYL
                     VHKQPLNSTEVIEGEGIQLPAGRCC"
     gene            3765..4262
                     /locus_tag="PKM72_06815"
     CDS             3765..4262
                     /locus_tag="PKM72_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNP60534.1"
                     /translation="MADNYSFDVISRIDMQEMKNVVDQTEKEVGQRFDFKGSKTELTL
                     KEKDKQLVVISDDDYKLNAVLDILKGKCVKRNVSLKGLTYGKVEEALGGTVRQTITIQ
                     SGLPEEKAKAITKSIKEAKFKAQGQIQGEQVRVQSKSKDELQAVMKHLKEQDFGLDLQ
                     FENYR"
     gene            complement(4201..4998)
                     /locus_tag="PKM72_06820"
     CDS             complement(4201..4998)
                     /locus_tag="PKM72_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60535.1"
                     /translation="MEKGSGEFLITMILVRIWFICLVTSILVGCGSGPPPRMIDLLGE
                     DAFAHGERPFQNNEWRQQSRIGLVVYSDATGQNAAPAIISEYLEILTRRTEKFLKQQC
                     VFHEIVVVPPLSNPVNLAQELKVQGQRLHVPYEIVVVFSSQEQAGPEKIGQATMMTLM
                     SGTVVENLSLAEVGILHVSDFHLVFRTVGRGTDSLEQLDVPIGSNRPTPIDARDILRA
                     QAAQHALDRALASLGSACQIEKKARTGFTDSFRIANRVRNPALSDAS"
     gene            complement(4971..5738)
                     /locus_tag="PKM72_06825"
     CDS             complement(4971..5738)
                     /locus_tag="PKM72_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60536.1"
                     /translation="MWRHIWLIVGLLCSVSSFGCSLDNTPKRLGAYLPQSSAQEGSVT
                     KTAIPAEGVTSILVVLNDSGFEKSAPVLRRGTLENLGEHLKTEIQKQVPIQITSVVYP
                     EDSKPNGSVDQFIQLAKEQNAPYLLLAVLSSSEWEVFAHLPLQGNQGGGLRSMGLPGY
                     RAENYARVELALLDGQTGRSLVSTDGQAWASLERLNVPLESNVYPVVRRYQNQPPIYP
                     NSESEEYETLRWVSGQDAIAQAVMHLEILWKKDQANS"
     gene            6277..7230
                     /locus_tag="PKM72_06830"
     CDS             6277..7230
                     /locus_tag="PKM72_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60537.1"
                     /translation="MNHTQTVDACGLPYTIPVGEIITKTFLKVGQERFNGVRAEPPVG
                     DAQGAPPDGYRVIVDLNQFLFDPVTLMATEPRYDAFVDLKLLVVYEDPKGTALAQTPL
                     SYHEKVSMWVPALSSSASSCATQQIDGTVEDATEALAKEMVSVMPRLGQVLSPVSTNP
                     YPNAEPSGTTPSSPLPMQNPAQTATPPKPAISPSVQFRTKLVDANRNLILEGGEAVIL
                     LIETTNVSGSTIPSAYVELRGTPILVEAFKRVAPIPMPIGELKAGEKRTAEIRGRIGE
                     VKESLKGELIIGIILSEGLPPGTHTILTEIQPRSSLKRPTR"
     gene            complement(8166..9668)
                     /locus_tag="PKM72_06835"
     CDS             complement(8166..9668)
                     /locus_tag="PKM72_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNP60538.1"
                     /translation="MRTFYQTFTRLTAAALIVAMSAAPGLAAKTTPATDKSTDVAAIP
                     RAIPDLVPYTAFDPPKGVFDASKLTLSGDMRVRPEFRTNGNFSNNGDSNAFFSSQLIR
                     LGFNYDVSPDVVFFLQPQVSNNWGSDSPSGNNPAGNITGAGNPGSTATDLFLRQGYMM
                     VRNFVLPNLTLKAGRQLLAWGDHRIFGTFDWNNVGFAFDGVTMRYNHAQFPVELGWLR
                     VAEGNCVPNAGGCTNGKASKGDGDILFVRMPMKFGSVAIEPAYIYEDGGSTTGGPGTS
                     VGGTATGPVGGQQSNQQRSTVGGRVAFKQGMFDATGEGYWQFGELGVPGQASQTRINA
                     LAGHLDGGITLPVPMQPRIGAEINYATGSDPKNGGHTFSQLFPTNHIHFGYMDLMSWQ
                     NMLTYGGNLQLRPTAESHFEIAGHIMRLANEKDNWYTASQATFFNTPANNKEKSLGGE
                     IDVVYTLFFQNNKVGWQVGYGHFFTGDYLKKNGFGTADQNWGYTQLWINF"
     gene            complement(10161..10448)
                     /locus_tag="PKM72_06840"
     CDS             complement(10161..10448)
                     /locus_tag="PKM72_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNP60539.1"
                     /translation="MFGLGAMEVLIILVIAFMLFGPKELPEIGKQIGKAVKGFKETTE
                     DLRQSVEPEINMITQEFKSVEQDLESSIKEAEEEIKGATEQATEPGGSKMG"
     gene            complement(10567..10851)
                     /gene="tatA"
                     /locus_tag="PKM72_06845"
     CDS             complement(10567..10851)
                     /gene="tatA"
                     /locus_tag="PKM72_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNP60540.1"
                     /translation="MELMLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSVAEADAMD
                     VTPNEQGEGGQVPPPQPEQLPSQHQTAEVPPPPQAQGVPQPTESTQSKQS"
     gene            complement(11122..12558)
                     /locus_tag="PKM72_06850"
     CDS             complement(11122..12558)
                     /locus_tag="PKM72_06850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNP60541.1"
                     /translation="MGASPSIDPDALPQLVGDFQPVDMWQAHINRIFYGLRGSRVREY
                     YQTVAAADFRLGFALAEDYWRRCGQRAKTQAADQATVPLIVMEWGVGNGNLAACFLDR
                     LQELDQEQLIFPRITYRCIEREPVLLEQAKANPDLAKHRDRVTFEPVSIEDLSACPDG
                     SIDRIICNELWSELPTKLILRKGGEIHEEQLRPNLNEKRLADFPDWAKFIEAFDQQDI
                     ESLHTLPSFLEDLVWEREYRPIETKTFPFRRTVADFLKHIDEEVLVPYNVGACQSIKE
                     AKRLLATNAIGFSSFDAGTVDPRVLNDPEKPCYTVQGGQFSFMVNFQLMLDVARHLGI
                     HTGMIESQRDFVGRSLSTNVLSVMDLLASHPSPPEGQDWKVEALILRTLEALNRTYVS
                     PYQRHIDFPLSESTPAQERAALENLVRSLPPHGVPDTIAYLTESEILKAMPDLQKLGY
                     DSEGIKDMLQLPPQPVDYTHMFFSSNGS"
     gene            12750..13472
                     /locus_tag="PKM72_06855"
     CDS             12750..13472
                     /locus_tag="PKM72_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60542.1"
                     /translation="MLEITNVESDNLYTRTNMIHPLPLMRWTFLLGTVIAIVHVSMPG
                     TAFCDDPPLPANSPSMLQPKNTQHGFFQYDTPPDHFSDGPAPYDSYSQSPLIPSLDQK
                     PAGVFLSLDFAESFEVKPTTRGGYTHIPVNPTERFLPHVPSVYLVFSVHEHLSSYQII
                     GRLFSESETVMDSPQWLDEDIVDLATEDESGFLKFFPPDGIWQPGRYRVEIFVGFIAN
                     DVSKMGTMRFTITPPPLAPPSP"
     gene            13682..13945
                     /locus_tag="PKM72_06860"
     CDS             13682..13945
                     /locus_tag="PKM72_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNP60543.1"
                     /translation="MLKKSGSYLLLFIFIGGLLGSILGEILQVVAPQGTVQNVFVQAL
                     NLGLDPPVTVNLVLIKFTLGFLLKMNLLTVLGMFLGAYVYKHI"
     gene            complement(14173..16182)
                     /gene="uvrB"
                     /locus_tag="PKM72_06865"
     CDS             complement(14173..16182)
                     /gene="uvrB"
                     /locus_tag="PKM72_06865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNP60544.1"
                     /translation="MMQATNTFSLKSDYTPKGDQGNAIAALTAGLEQGLKHQVLLGVT
                     GSGKTFTMANVIVNVQKPTLVVVHNKTLAAQLYQEFKSYFPDNAVEYFVSYYDYYQPE
                     AYIPTTDTYIAKDSAINDTIDQMRHSATTALLQRNDIIIVASVSCIYGLGSPEVYHDM
                     LLYLEPGMEIRRERILAKLVDIQYSRNDLELQRGMFRARGDVIEIYPASSDSNTVRVE
                     LFGDEVEALYEIDPLTGNALRKLPKIAIYPKSHYVIAPDRYEQAIAGIESELEERLAY
                     FRRTNQLLEAQRIEQRTRFDLEMIRTMGYCHGIENYSRHLSGRAPGEPPPTLLDYFPK
                     DFLLIADESHATIPQFGGMYEGDRSRKNTLVEYGFRLPSAMDNRPLKFPEFEGCMNQV
                     VYVSATPGPYELKHAGKALVEQIIRPTGLIDPVMEVKPAKGQVEQLLGEIRLRVAKGH
                     RVLVTTLTKRMAEDLTEYYHDQGLKVRYLHSDIKTLERADIIRDLRRGVFDVLVGINL
                     LREGLDLPEVSLVAVLDADKEGFLRGYRALVQTAGRAARHRDGTVILYGDVITDSMRR
                     TLDETGRRRTIQLAYNAEHGITPETIIKRIHDLEYQLAEADYVDVSVAAEAIAAYGGE
                     KDLEQSITALEKDMKAAAKALEFERAAQLRDTIRVLRQKQIQVGI"
BASE COUNT         4097 a         4359 c         3793 g         4109 t
ORIGIN      
        1 gcctggtatt tttatgttgg aaatccggag aagaacaggt tcgctggtgg cacgacatcg
       61 acagcggata tggaaacaga caacccattg aagaccagaa tccatagacc tgccccgcat
      121 tcgcctattc gtttcaagtc cattatccag accaccagat cttcttccga cgaaaaataa
      181 ccatacgatt gccttctgaa aatgatgaaa ccctcatggc ccttgacttg taaaggggag
      241 gcaattcatg cggcttcggt aaagaaacga ggaaaaagaa tgaaattcgt gattctagga
      301 aaagatggtc ccgaggggtc ccaaaaacgc ccactttatc gagacgccca tctcaaacgg
      361 cttgaagcat gggcacaaca agggaaaatt attttggctg gtcctctgac cgataaaatg
      421 ggaagcctaa tcgtcgttga gtcagaatca ctcgaagaag tccaagagtt tgctaagaat
      481 gacccctata ccctttttgg tgtttttcag gaagtcaccg ttcacccttt tttacaggta
      541 tttcccacag cgaatcattc accaaaacca ggaaaataga ggaagaattc agaaaaaacc
      601 ttgtaaaaac cctgagtagc taccttttgg taggctcggt ctttttcgaa acaaaatcca
      661 atactcgttg ccattcatcc ggacgcactg gttgaaccga gagcctggaa cctttacgaa
      721 gaagttccat gttccctagt cctttaatgg ctcgcaactg atccaaaggc aaggcctgag
      781 gaaacttttt cacgaaccgc acatccacaa gaaaccaacg gggtgattcc agggtactgc
      841 gcggatcgaa atatcggctc cttgaatcga atgcaaatga gtctggataa gcggccttaa
      901 ccacttcaat gattccgact atggctggtg ggtccgtatt gctatggtaa aaaaatccta
      961 aatctccaac attcatggat ttcaggaaat ttctagcctg gtaattacgt acgccttccc
     1021 aacatgtggt ctgccgtgga gatttaacaa gatcatcaat ggaaaatgtt gtcggctctg
     1081 atttgaacaa ccaatactgt gacatcatgt ctcactcatg gttttgaaaa ccacaaacac
     1141 cctattgaat taatactaat accattgagc aattataaag tgtatatttt tcagagtaca
     1201 accgactctt atctgatgca tctaccgtag gattgcttaa tatgtatgaa aaatggttcc
     1261 aacaatgatt gatcaaataa ttcagtatgc cgaaacttgg gttccccgtg aaatctggat
     1321 cggcatgatc accttttctt tcattgcctt tgtaggaacc ctcattgcaa tccctgccat
     1381 attaattcgc cttccaccgg attacttcga taatcatggc catcgcaagt ggtttgccaa
     1441 tcatcatccc ataattcgca cccttgggtt gatggtgaaa aacggcctgg gactcatttt
     1501 tctggtagcc ggaatcgcca tgctggtttt accaggacaa ggaatcctca caatggtcct
     1561 gggaatcttg tttctggatt ttccaggaaa acaccgggtc gaacaaaaac tcattcggca
     1621 accacagatt ctcaacgcca tcaactccct gcgaaaaaaa gcaggcaaac tcccgtttgt
     1681 gtttcatgaa aaatcaccga cctaatcaca tgtcgacctg aacacctctc cgtcgccacc
     1741 cgtttacacc cttttctccc attgctagga tgccccgcat gtcattgtgg aataagatcg
     1801 gatggctagg aatcgccgga ctttgtgccg tgggattcgg ccatgtggtg ggcattatca
     1861 atcctcacga aaaagttaac gggttgtggc tcgtggtcgc tgcctcatgt ttttatgtgt
     1921 tggcctatcg gttctatggc cggtttttag cgcagcgagt catgaacctg gatgaccgtc
     1981 gccggacacc tgcgcacaga ctggaagacg gcaccaactt ttatcctgcc aacaaataca
     2041 ttctcttcgg ccatcatttt gcggccatcg ccggagcggg cccgttactc ggtccggtct
     2101 tggcagcaca atttggcttt ctcccggggt ttttatggat tgtcatcggt gcggttctgg
     2161 caggcgcggt ccaggatttt attattctcg tggcctccat gcggcgaaat ggtcgttcac
     2221 ttccggaaat cgcccatgct gaattgggtc ccattaccgg cctggccaca gccattgccg
     2281 tgcttttcat cgtggtcgtc gccctggccg ggttaggact ggctgtggtg aacgcgcttt
     2341 accacaatgc ctggggcacc tttaccatca tcatgaccat tcccattgcc gtgttcatgg
     2401 gactgtacct tcaaaaattt cgccaggggc aggtggggga aatgaccctc atcggattag
     2461 ggctgttagt gctggccatt atcgtcggcc gaaccgtggc ccaatcagat tgggcagaat
     2521 ggttcctctg ggatcgcacg acgctcacct ggagtctcgc ggcttatggg tttcttgcat
     2581 cagtcctccc cgtctggatt ttattagtcc cgcgagatta tctctcaaca tttatgaaat
     2641 taggtgtgat cgcccttttg gccatcggcg tcattgtgct cgcccccacc attgagatgc
     2701 ctcgcaccac catttttgtg gccggcaatg gaccaatcat tcctggaaca ttgtttcctt
     2761 ttctctttat caccattgcc tgtggggcta tttccggatt tcattccctg gtctcttcag
     2821 ggaccacgcc caagctgatt agtctggaat ctcaggcagt tgtcgggtat ggcgccatgc
     2881 tcctggaaag ctttgtcggc gtgattgcgc tcattgccgc ctgtctcctc attcccggcg
     2941 actatctcgc catcaacacc aacctttcgg ctgaaaccct ggagaccatg ggatttccga
     3001 ccgcccacat taaggaactc tccacccttg ttgaagtcga tgtatctggc cgtcccggag
     3061 gtgccgtgtc cttagctgtt ggcatggcct cgattttttc cagcttgccg ggaatgtccg
     3121 ggctcatggc ttactggtat caatttgccc tcttattcga agccctgttc attttgacca
     3181 cgattgatgc tggcacccga gtcgcacgat acctggttca agaattaggg ggacgagtct
     3241 atgctccgct caaacagatc aattggtggc caggggtatt gggcgccagt ctattcgtcg
     3301 ttggagcgtg gggatatctc attggcaccg gatccattgc gaccatctgg cccatgttcg
     3361 gggcggccaa tcaattactc ggcatgcttg cgctttgtat tgccacaacc gtcctcatca
     3421 agatgaacaa aacatcctat ctgtgggtga ccgtgatccc gatggtgttt gttggagtga
     3481 tcacgctggc cggatgctat gaactcctgg tgatctttat cagtcgggcg ctctcagccg
     3541 atgggtcgca aagcctgacc atgtccatca atgcctcact cgtcggctta gtggccatac
     3601 ttgcactcat tgttctcacg gacagcgcca gaaaatggta tggctatctt gtgcacaaac
     3661 agccattgaa tagcactgaa gtcattgaag gcgaaggcat tcaacttccc gcaggacggt
     3721 gctgttaaac caaggacctg caaccataac ttgtaaggag gcttatggca gacaactatt
     3781 ccttcgacgt catttcccga atcgacatgc aggaaatgaa aaatgtggta gatcaaaccg
     3841 aaaaagaagt cgggcaacgc tttgatttca aaggatcaaa aaccgagctc acgttaaaag
     3901 agaaagataa acaattggta gtgatctccg atgatgacta caaactcaac gcggtcttgg
     3961 atatcctcaa aggcaaatgc gtcaaacgca atgtctcgtt gaaaggcctg acgtatggga
     4021 aagtggaaga agcgctaggg ggaaccgtcc gccaaaccat taccattcaa agcgggcttc
     4081 ccgaagaaaa agccaaagcc atcaccaaat cgatcaaaga agccaaattc aaggctcagg
     4141 gacaaattca aggggaacag gtcagggtgc aaagcaaaag caaagacgag ctgcaagcgg
     4201 ttatgaagca tctgaaagag caggatttcg gactcgattt gcaattcgaa aactatcggt
     4261 aaaacctgtc cgcgctttct tttcgatctg acaagccgac cccaaggaag caagtgcccg
     4321 gtccagagca tgttgagccg cttgagccct caaaatgtca cgggcgtcta ttggggtagg
     4381 acgatttgac ccaattggca catccaattg ttccaagctg tcggtccccc gccccactgt
     4441 ccggaagacc aagtggaaat ccgatacatg gaggattcct acctcagcca gcgataaatt
     4501 ttctacgaca gttccactca tgagtgtcat catcgtggct tgcccaattt tttcagggcc
     4561 ggcctgctcc tgacttgaaa acacgaccac gatttcatag ggaacgtgta agcgttgtcc
     4621 ttggactttc agttcttgag caaggttaac cggattggat aagggtggaa cgaccactat
     4681 ctcatgaaaa acacattgct gcttaagaaa tttctccgtg cgtcgggtga gaatttcaag
     4741 atactcagaa atgatcgccg gagcggcgtt ctgccccgtg gcatcggaat acactaccaa
     4801 cccgattctt gactgttgcc tccactcatt attctgaaat ggacgctccc cgtgcgcaaa
     4861 ggcatcttct ccgagaagat caatcatccg gggtggggga cctgacccac atcccaccag
     4921 aatagaagtc accagacata tgaaccatat tctgacaagg atcatggtga tcaggaattc
     4981 gcctgatcct ttttccataa aatttctaaa tgcattacgg cctgggcaat ggcatcctgg
     5041 ccggaaaccc agcgaagagt ttcatactcc tcactctctg aattgggata aataggtggt
     5101 tggttctggt accttcggac aacgggatag acattagatt caagcggaac atttaatcgt
     5161 tcaagggagg cccaagcttg accatccgta gagaccaacg atcggccggt ttggccatca
     5221 agcaaggcta attccacccg ggcgtaattt tcggcccgat agcctgggag ccccatactt
     5281 cgcagccccc ctccttgatt gccctgcaac ggaagatggg caaatacttc ccactctgaa
     5341 ctggacaaca ccgccaagag aaggtaagga gcgttctgct cctttgccaa ttgaatgaat
     5401 tggtcaacag agccattggg ctttgaatcc tcaggatata cgacggaagt gatttgaatc
     5461 ggcacttgtt tttgaatttc cgttttcaga tgttccccga gattctccag ggtcccacgg
     5521 cgaagcacgg gagccgattt ttcaaatccc gaatcgttca gaaccaccaa gatactggtc
     5581 acgccttcgg ctggaatcgc cgtttttgtc acgcttcctt cctgagcgga ggattgaggg
     5641 agataagccc ccagacgctt tggggtattg tccaacgaac aaccaaaaga agaaaccgaa
     5701 cacagcaacc cgacaatcaa ccagatgtga cgccacatgg tgaagcctcc aacggttaag
     5761 gtaacatttg taataacgat taattgtctt ctcccggatc aggaatcgtc aagaacaaga
     5821 aagtgagaaa cccaataggt gttgtttcga agaacaacaa agagagcgag acgcaaggcg
     5881 gattccagaa ttcatcgttc cctctctgga tcatttctgt taggcggaga gaagtccatt
     5941 gcccacggtc tctttgtttc tccgtctttc ccaaagcctg ttatccggaa attatattag
     6001 ttggtttttt tcaattaaaa cccgtctcgg atttcaatca acgggaaaga ccgcctacga
     6061 gtaaaattca ggatcttgtt gccatctttc aagatcttgg ggtagggtag atctgtgtgc
     6121 ccaatcataa ataaaatctt tcaatacagc ctattagctg ttttattgtc cctccttttc
     6181 gcctgcgaca aagtttccat tccccgtttg actttcgacg atcgtgcaga accaagaatt
     6241 cctttcccga ttacctatgc attctcgccc gacctcgtga accataccca gacggtggat
     6301 gcgtgcgggt tgccctatac gattccggta ggtgaaatca tcaccaaaac ctttctcaaa
     6361 gtcggccaag agcgcttcaa cggagtcagg gccgaacctc cggttgggga tgcccaaggc
     6421 gctcctcccg atggctatcg agtaatcgta gatctcaatc aatttctttt tgatccagta
     6481 accttaatgg caacagaacc ccggtacgat gcctttgtgg atctgaaact tcttgttgtc
     6541 tatgaagatc ccaaaggcac cgccctcgct caaactccct tgtcctacca cgagaaggtc
     6601 agcatgtggg ttcccgccct ctccagctca gccagctcat gcgccacaca gcaaattgat
     6661 ggaacagtgg aagatgccac cgaagcgcta gccaaggaaa tggtaagcgt aatgcctcgc
     6721 ttggggcagg tattgtcccc ggtttccacc aatccatacc ccaatgccga accgtcaggt
     6781 accacaccat cttctcccct tcctatgcag aatcctgccc aaacagctac gcctcccaaa
     6841 cctgccataa gcccttccgt ccaattccga accaaactgg tcgatgccaa ccgaaatctc
     6901 atcctggaag gaggcgaggc ggtgatcctg ttaatcgaaa ccacgaatgt cagcggatca
     6961 acgattcctt ccgcctacgt ggagctacgc ggcacaccca tactcgtcga agccttcaaa
     7021 cgtgtggctc ctattcctat gccaatcggg gaattgaaag ccggcgagaa acgaacggct
     7081 gaaattcgag gtcgcatcgg ggaagtcaag gaatctctta aaggagagct gatcatcggg
     7141 attatcctat ccgaaggcct gccacccgga acccatacca ttctgactga gatccaacca
     7201 aggtcttcat taaaaaggcc tacccggtaa gatccctcct gcctataaat agaattccac
     7261 cataaagcag aagcttattg ggaaaggtgg tacagagacg agggaaacct tttgcataaa
     7321 aatcctacac acatgggtat caaaaaacct gaaacttcag ccttgggtct ggatcaacag
     7381 cattcgcccc taaagatagt cagggagagg gctgaatgcc agtgactggc gaggcaactg
     7441 ccaacacaaa tgttgttcgg cgctcggcat accatttggc aactcaacca gaaagaaaac
     7501 atccctgact tacgctggcg gtatctgtcg aaggggatga attggtctgg ccaatacccg
     7561 gcctaagctc aatgctattg cgcatcgctt gaaaactgga ggagaacatg tcaggactgg
     7621 ggggagccca ttggaggtgt ttatacaact gcgccatcaa tcagtcgctg cacttggaac
     7681 ttgaatccgc cgatttaact ccgaaatgaa cggagggatc attgaataaa atcgaattat
     7741 tgttagcata ggtgtatcga ttgaagtcct ggaggttgcc tggatttggt atgaacacat
     7801 ccgctgagat aaaccgaacg agggtgcgtc gtagttgcga gcttcataaa agtagagtcc
     7861 catgctttca tccaattcct tgtccgtata tttgcaggcc acattgtcgt tgcccgtatt
     7921 ggcgaatcta ttcccgtaga aaatgcaacc aagtttttcc tacacccccc agacatacat
     7981 ttaaggagga ttaaccaaag tagaaaccga atcagggatg atgaattcag gccggagatt
     8041 cattggaaaa gcagaaaaaa ggccatccga agcaaatagc cccgacagaa aaaagcccag
     8101 ctggattaat ccagccgggc ttttcaggat aacggggagg gttaagtccc ctaagataag
     8161 gatgattaga aattgatcca gagctgggtg tagccccagt tctgatcggc ggtgccaaat
     8221 ccattcttct tcaagtaatc gccggtaaag aagtggccgt aacccacttg ccatcccact
     8281 ttattgtttt ggaagaacaa ggtgtacacc acgtcaattt caccaccgag agacttttct
     8341 ttgttgttgg caggagtgtt aaagaaggtg gcctgagaag ccgtgtacca gttgtccttc
     8401 tcgtttgcca atcgcatgat gtgcccggca atttcgaaat ggctttctgc agtcggacgc
     8461 aattgcaaat ttccgccata ggtcaacata ttctgccagg acatcaagtc catgtacccg
     8521 aagtgaatgt ggttcgtcgg gaacaattgg ctgaacgtat gtccaccatt cttaggatcg
     8581 ctaccggtcg catagttgat ttccgctccg atacggggtt gcatgggaac cggtaaggtg
     8641 ataccaccat ccaaatgccc cgccaacgca ttaattcgcg tttggctggc ctgtcctggg
     8701 acccctaact ctccgaactg ccaataccct tcaccggtgg catcaaacat gccctgcttg
     8761 aacgcgacac gaccaccgac cgtgcttcgt tgttgattgg attgctgacc acccactggg
     8821 cccgtggccg ttcctccaac agatgtcccc ggcccaccag tggtcgaacc accatcttca
     8881 taaatatagg caggctctat ggctaccgaa ccaaatttca tcggcattct cacaaataag
     8941 atatccccat cacccttcga ggccttgcca ttcgtgcatc caccggcatt cggaacgcag
     9001 ttgccttccg ccacgcgcaa ccaacccaat tccacaggga attgcgcatg gttataccgc
     9061 atggtcaccc cgtcaaatgc aaacccgacg ttgttccaat cgaatgtacc gaagatccga
     9121 tgatcgcccc aggccagtaa ctgtcgaccg gccttcaatg ttaaattcgg aagcacgaag
     9181 tttctgacca tcatatatcc ttgacggaga aacagatcgg ttgcggtact acctggatta
     9241 cctgcaccgg ttatatttcc ggcgggattg ttgccagatg ggctatcact cccccaatta
     9301 tttgaaacct ggggctgcag gaagaatacg acatcgggag agacatcata gttgaatcct
     9361 aagcggatta attgactgga gaaaaatgcg ttgctatccc cattgttact aaaatttccg
     9421 tttgttctga actctggtct cacacgcatg tcaccggaaa gtgttaactt gctggcatcg
     9481 aatacgccct taggtggatc aaatgcggtg tagggcacta aatcgggaat ggcccgcggg
     9541 attgccgcga catcggtgct tttgtcggtc gccggagtcg tcttggcggc caaccctggc
     9601 gctgcagaca ttgccacaat caacgccgct gctgtcaacc tggtaaaggt ttggtaaaaa
     9661 gtcctcattg cactcctcct gttggtgaaa aacccctgga ctcaagcgcc aacattctgt
     9721 accccagatt tttttcagaa ctgttggact ctctcatccg cttccttctt gactgcgtaa
     9781 tatttgccgt ctcaagcact aggatgcctt ctgcaatcaa acagttccct attctaggac
     9841 tcctcgattt tgttcctgtt tgccatttag atgccaagtc tccttatttg ttgtaaatca
     9901 ttaaaaagca atgtctttta cataactttc ttcatgattt tatttctttg ccctatcgct
     9961 tgaatgctac gatagttgca aaagcacaac acgacagaag atagctgtag acccgcccgc
    10021 agtaagtacg atgaacagtc tgatgaccgt ccggaaggaa ttcgtgatga cacgagagaa
    10081 attctaaaag ctgcaataat gcctgagtac caaccaacga cttagcaatc aactccttca
    10141 tggagccgca cctaaccggg ctatcccatt ttagacccac cgggctctgt ggcttgttct
    10201 gtcgcccctt tgatctcttc ttcggcttct ttgattgacg attctaaatc ttgttcgacc
    10261 gatttgaatt cctgcgtaat catattgatc tccggctcaa cagattgccg aagatcttca
    10321 gtggtttctt taaacccttt gaccgctttc ccgatctgct tgccaatttc gggtaattcc
    10381 ttgggaccaa atagcatgaa cgcgatgacc aggatgatca aaacctccat cgctcctaag
    10441 ccaaacatct tcccactcct ctatggtctg cccgctaaat gtataccttg agcgttgatc
    10501 aggatttggt tcctgttgtc tgtcccgtcg gtgggcggtc ttcaccgtat cagtctcacc
    10561 cgagaattaa ctctgtttgg attgcgtaga ttccgttggc tgcggcaccc cttgcgcctg
    10621 tggcggaggg ggaacctctg ctgtctggtg ttggctcggc agttgttcag gttggggtgg
    10681 tgggacctgt ccaccttcgc cttgctcatt aggcgtcacg tccatggcat ccgcttcggc
    10741 cacagacttt ttaaagccct tgatcgcttt gcctaagccc tcaccaagct gcggtatttt
    10801 tcctgcaccg aaaataatga gcacaatgat gaggatgagc ataagctcca tccatccaaa
    10861 cgatccaaac acagcaacct cgtcttatgg taaatgagtg tctacgttat tagaaaaatc
    10921 cgccgcagta tacacaacac atttaaaaaa atctacctga ttgcaaattt cctgcatccg
    10981 ccattatttt tttccgattg gggatatgcc ttcacagtat ccactcccgc gcggacagtc
    11041 tttcaactcc accatcatta accgcgttct gccttcatca ttccaactcg acgagtagta
    11101 tctcgcgaca gtgaaaaata attaggaacc gttagaggag aaaaacatgt gggtgtaatc
    11161 caccggttgt ggcggaagtt gcaacatgtc tttgatgcct tctgaatcgt atcctaattt
    11221 ttgcaaatcg ggcatggcct tcaaaatttc tgattccgtg agataggcga tggtatccgg
    11281 cacaccatgt ggcggtaatg aacgcaccag attctctaat gccgcgcgtt cctgggccgg
    11341 ggtactttca gaaagtggaa aatcgatatg gcgttggtat gggctcacgt aggtgcggtt
    11401 cagtgcctcc agggtacgaa gaattaacgc ctccactttc cagtcctggc cttccggcgg
    11461 cgaaggatgt gatgccagca gatccatgac actgagtaca ttggtcgaca agctccgtcc
    11521 cacaaagtcc cgttgcgatt cgatcatacc ggtgtgaata cccaaatgcc tggccacgtc
    11581 tagcatcagc tgaaagttca ccatgaagct gaattggcca ccctgtacgg tatagcaggg
    11641 cttttccggg tcattcagca cacgaggatc gaccgtccct gcatcaaaac tgctaaatcc
    11701 gattgcattc gtggccaata atcgcttggc ttccttaata ctctgacatg cccccacgtt
    11761 gtaaggcacg aggacttctt cgtcgatgtg cttgagaaaa tccgcaaccg ttcggcgaaa
    11821 cggaaaggtt tttgtctcga tggggcgata ttcccgctcc cagacaaggt cctcgagaaa
    11881 cgaaggtaac gtatggagag attcgatgtc ctgctgatca aatgcctcaa taaactttgc
    11941 ccaatcaggg aaatccgcta accgcttttc gttcagattg gggcgaagct gctcttcatg
    12001 aatttcacca ccttttcgaa gaatgagctt agttggcaac tcgctccaca gttcattgca
    12061 gataatccga tcaatacttc catccggaca ggcagacaaa tcctcaatcg aaacaggttc
    12121 aaacgtgacc cggtcacgat gcttggccaa atcaggattg gctttggcct gttccaacaa
    12181 gaccggctcg cgctcaatgc atcgataggt aattctaggg aatatcagct gttcttgatc
    12241 gagttcctga aggcgatcca ggaaacaggc tgccaggttc ccatttccga caccccattc
    12301 catcacgatc aatggtacgg tcgcttgatc ggcagcttgc gttttcgctc gttgtccaca
    12361 ccgacgccaa tagtcttcgg ctagagcaaa ccctaaccgg aaatcggctg cggccaccgt
    12421 ttggtaatac tctcgaacac gacttcccct taacccgtaa aatattcgat taatgtgggc
    12481 ttgccacata tccacgggtt gaaaatctcc tacaagttgc ggcaatgcat cgggatcaat
    12541 acttggtgaa gcacccatag ttctaaccta ttattcctta gacagaatga atgataatgc
    12601 ctgtggcaac gaagtagatg aaaatgcgta gaatcgcccg gaatcttagc aaacacctcc
    12661 tggtgccgca aggtaagaac gaaagtcctt gactcgatgt cgccatcaaa ggtagagaac
    12721 gtggactcat caggaacagc ataaaaagaa tgttggaaat tacgaatgtg gagtcagaca
    12781 acctctacac caggaccaac atgatccatc ctttgcccct catgcggtgg accttcctct
    12841 tgggcaccgt gatcgcgatc gttcacgttt caatgcccgg caccgctttt tgcgatgatc
    12901 ctcccctccc ggccaactcc ccatccatgt tgcagccaaa aaacacacaa cacgggtttt
    12961 tccaatacga tactccgccc gatcatttct cagatggtcc ggccccttat gatagttact
    13021 cgcaatcgcc gctcattcct tcattggatc aaaaacccgc cggcgtgttt ctcagtttag
    13081 atttcgcaga aagcttcgag gtcaaaccca ccacgcgtgg cggctataca cacattcccg
    13141 tcaaccctac cgagaggttc ttgccacacg ttccctccgt gtacctggtg ttttctgtcc
    13201 atgaacattt gtcgtcctat caaatcattg gccgcctgtt ctctgaatcg gaaaccgtga
    13261 tggactctcc tcaatggctc gacgaagaca tcgtcgatct tgccacggaa gacgagagcg
    13321 gatttttgaa gttttttcca ccagacggaa tctggcaacc aggccggtat cgcgtcgaaa
    13381 tcttcgtggg gttcattgcc aatgacgtca gcaaaatggg caccatgcga tttacaatta
    13441 ctcctccccc tttggcaccc ccctcaccct aaacgcaccc gtgtagccac aggcctttcg
    13501 catcaggcta tttcttgact caacttcagc cttcttgtaa tgtattcagg agtttaccct
    13561 aagcatattt ttggtcaatt ccaaccatcg cgttgtccga ccataccttg aacattgaac
    13621 attccaagcg ttcatgcaca ggcaattccc tgatctctaa caatccgcca ggaggcaatg
    13681 ggtgttaaaa aagagtggga gctatttact cttatttatt tttattggcg gactgttagg
    13741 cagtatttta ggggaaattc ttcaggtcgt ggctcctcag ggaaccgtac aaaatgtttt
    13801 tgttcaggcg ttgaacttgg ggttagatcc accggtaacc gtcaatttgg tcttgataaa
    13861 attcaccctc gggttcctcc tgaaaatgaa cctcctcacc gtcctgggta tgttcctcgg
    13921 cgcctacgtg tataaacaca tctagcaggg agttaagaaa gtccgccagc agcgttcccg
    13981 gccttcgttg gtcctccttc gccgaagtag ctacgcaggc tgaaagcggc ttcatgtcta
    14041 gcgcttcgcc gcgcaagggc gcaagaaggc cggctctttg tcggactcac gtactcactc
    14101 ctacgctcca cccaccaaag tgcctgcggc cttcccgtcg aaagacccaa gggcagatgt
    14161 agaattttcg aattagatac cgacctggat ctgcttttgt cggagaaccc gaatggtatc
    14221 ccgcaactga gcggctcgtt caaattccaa ggccttggct gccgccttca tatctttctc
    14281 caatgccgtg atgctctgtt ccaaatcttt ctcccctcca taagccgcga tagcttctgc
    14341 cgccacggac acgtccacat agtccgcctc tgccaattgg tactccaaat catggatacg
    14401 tttaatgatc gtctcagggg taatcccatg ctcagcattg tatgccaatt gaatcgttcg
    14461 tctacgtccg gtttcgtcta acgttctacg catcgaatcg gtgatcacat ctccatagag
    14521 aatcacggtg ccatcgcgat gacgggcagc gcgtccggcc gtctgcacca gggcacgata
    14581 cccccgcaga aaaccttcct tatccgcatc caacaccgcc actaaactga cctcgggcaa
    14641 atccaaccct tcccgcaaga gattaatacc gaccaggaca tcaaagacac cacgccgcag
    14701 atcccggatg atgtccgccc gctcaagcgt tttgatatcc gaatgcagat agcgcacctt
    14761 taatccctga tcgtgataat actcggtaag atcctccgcc atgcgcttgg tcagggtcgt
    14821 caccaggacc cgatgtccct tggccactcg cagccggatt tccccgagca actgttcgac
    14881 ctggcccttt gcaggcttta cctccatcac aggatcaatc aggcctgtgg gtcgaatgat
    14941 ttgctcaacc agcgcctttc cggcgtgctt cagttcataa ggtcccggag tcgcggagac
    15001 atacactacc tgattcatgc atccctcaaa ctccggaaat ttcaagggcc ggttatccat
    15061 cgccgagggc aagcgaaacc cgtattcgac caaggtgttt ttgcgtgacc ggtctccttc
    15121 gtacatgccc ccgaactgtg gaatggtggc atgcgattca tccgctatca gcaaaaagtc
    15181 tttggggaaa taatccaaca gtgtcggtgg cggctcaccc ggcgcccgtc cgctcaggtg
    15241 acgggaatag ttctcaatcc cgtggcaata ccccatggtt cgaatcatct ccaggtcaaa
    15301 tctcgtccgt tgttcaatcc gttgggcttc aagcaattga ttcgtccggc gaaaataagc
    15361 aagacgttcc tccagctctg attcaatgcc ggcaatggcc tgttcatagc gatcgggcgc
    15421 aatcacatag tggctcttgg gatagatcgc gattttcgga agcttccgca atgcgttgcc
    15481 ggtcaacgga tcgatttcat acagggcttc cacttcgtcg ccgaacaatt ccacccggac
    15541 cgtattcgaa tcggacgaag ccggataaat ctcaatgaca tccccccgcg cccgaaacat
    15601 tccccgctgc aactccaggt catttcggga atattgaatg tccaccagct tggccagaat
    15661 gcgctcacgg cgaatctcca tgccgggttc cagatacaac aacatatcgt gatagacctc
    15721 aggcgatccg aggccataaa tacacgatac ggacgccaca ataataatgt cattgcgttg
    15781 caggagcgcc gtcgtggccg aatggcgcat ctggtcgata gtgtcgttaa tagcggaatc
    15841 tttggcgata taggtatccg tggtcggaat ataggcttcc ggctgataat agtcgtaata
    15901 gctcacaaaa tattcgaccg cattgtcggg gaaataggat ttaaactctt gatagagctg
    15961 ggcagccaac gtcttattgt ggacaacgac caatgtgggt ttttgcacgt tgacgatcac
    16021 attggccatg gtgaaggtct tccccgaacc cgtgaccccc aacaacactt gatgcttaag
    16081 cccttgctct aagccagcag tcaacgcggc aatcgcattc ccttgatcac ctttgggtgt
    16141 gtagtcggac ttgagagaaa aggtgttggt tgcttgcatc atgacaatgt atcacaaggc
    16201 aaacaggagg ggcaggcagg caggcatgta tgcacacggt gtgatcgata gctgaagagt
    16261 ggctgctgga gagaatcccc tgttgagggt gaccccctga gggtagaagt aaagaaggtt
    16321 tgcgcggcga ccgaggagta atgccgtgga ttctcttg
//
LOCUS       DAORAQ010000054        16081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_618_length_16081_cov_65.819169, whole genome shotgun sequence.
ACCESSION   DAORAQ010000054 DAORAQ010000000
VERSION     DAORAQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16081
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_618_length_16081_cov_65.819169"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKM72_06870"
     CDS             complement(<1..619)
                     /locus_tag="PKM72_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60545.1"
                     /translation="MWWPLATSWMLMGLEFPLVTATVSRLVDPEIHLAAYGGVVFPLS
                     LMIEAPVIMILVASTALCKDWLAYRLIRNFIVVLGSILTVIHVVVAFTPVYGIMVQDV
                     LGVPEVIVEPARMGLQCMTPWTLSIAVRRFLQGVVIRAGQTRLIGLGTLVRLIVSSAV
                     LLGGLAVSSFPGIVVATVAMSSGAMVEAGFILIFVRPILRNLKASP"
     gene            complement(896..3469)
                     /locus_tag="PKM72_06875"
     CDS             complement(896..3469)
                     /locus_tag="PKM72_06875"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNP60546.1"
                     /translation="MSKLIEALNRLQSVRTEKELPSSPSLLNDIRQDEFECVQQHQSD
                     SKPSPKKNIHPEEGPRFNLDLREWLGILHHEYLGSFVREGGGAVKFAVLPGEDVLRTC
                     QQELEGLAKREGYVLARVDARFTKAHMVERLFQKIAKQIDWDELAYRFLQRLLEEHGH
                     QIPSDRQEFSLRQVAMVNERKEPMLRRDIQTWMEKAIEGDSGLCREFRMAMIRLCMAQ
                     LDAGDSDRVLATAVKEWLCGDLRLVTGVKKALIYQKISRHNARHMLSSLTRWLRLTGQ
                     GGLLLSLDISRYFIKKDALPTDGSLSFSPSATMDLYELLRQFIDSADEMEGLLMVILA
                     PQDFLTDARRGVDRYEALKLRVWDDVRPKHRQNPLSSLVRIQDDHESVSEGETGSSTG
                     MPRRMAPDGEARRVIEGLRAGVPNRHVVTTLGCLQPEVEGRFRRLLEATQQNITTGPC
                     PRGMVIEGEFGSGKSHILEYLQNLALDANFICSRIVISKETPLYHPVRLYYSAIEAAV
                     IPDKRGEVFSEIAGQCDVWAPRYKELVTWVNNPESQIDSRFAATLMLYERLSSDMELG
                     HRMTRFWTGDPIGVGDLKKYLQEAGFGERYAFGKISAAELALQRFQFAARLMQAAGYA
                     GWILLVDEAELIGRYSVNQRAKSYAEVARLMNLDGGPTLPGLGTVLALTNDFREEVLE
                     KKGDKIKLLEKLRAKNTEDERILADKAEKGMGLLESQRIPLGQPPNDIIQEAYQTIRS
                     LHLKGHGWHMWENAANDPAQIVPGTSMRKYVKSWVTHWDSLRFYPGEECEIEASTWKP
                     SCVDQVNLQEEDVQEESHAGRFASDPESVTGESPEEQEFVAHGIRGVPDSSTNLPQSP
                     L"
     gene            complement(3584..4324)
                     /locus_tag="PKM72_06880"
     CDS             complement(3584..4324)
                     /locus_tag="PKM72_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60547.1"
                     /translation="MSSSKRPPKEIHGHPRVSVPVPNWKIEETYPCAQSLNDTFWRWE
                     FLRRRSDYRKDWEQHYLQTYDFDLSCAKDPQYPTRYRKKVFSPDHPAFKARMPNSLEK
                     YHLSGLPNPSIAKPWMLSFDSNYGRIYFGQGPDWLGGGEEVTLCLSEWRMAAVFDLKK
                     PLSGQIEKVKADLMEWQKHQVGRSLERRKCRDEWPMYLRVLDAVDLGVPFHEIGRTLF
                     NLHDDEICEARANQLFKRACQISVHFPV"
     gene            5113..5739
                     /locus_tag="PKM72_06885"
     CDS             5113..5739
                     /locus_tag="PKM72_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNP60548.1"
                     /translation="MSDFERSITEAQTPRPAPTTVILFHKPKGYLVTRKDERERKTVY
                     HVLPPFLLQEGWVPIGRLDKDSRGLLLFTQNGTMVDRLTEPGGCEKTYAIEIRGRISD
                     NDLSRVLQGVSTSIGPLKVHRITKKREVGPKTQLEVVLREGKNRHLRRIFHALKDPKF
                     HTPLKVLDLKRIQIGPLTLDIPVGAWRFSTPDEETQLLAIFKIPIHPG"
     gene            complement(5759..7447)
                     /locus_tag="PKM72_06890"
     CDS             complement(5759..7447)
                     /locus_tag="PKM72_06890"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNP60549.1"
                     /translation="MSSLDQHSSIDFIRAKVSSDLERQAIPHQVHTRFPPEPNGHLHI
                     GHAKSICLNFGIAQEFGGLCNLRFDDTNPTKENMDYVSSIQEDVRWLGFDWGDRLFFA
                     SDYFEQLYQFAVRLIQTGHAYVDSLTAEEIRVYRGTLTEPGKDSPHRNRTIEENLALF
                     TRMRAGEFGDGVYVLRAKIDMTSPNINLRDPALYRIRHATHYRTGDDWCIYPTYDFAH
                     PLSDSLEGITHSLCTLEFEDHRPLYDWLLQVCEVSCYPQQIEFARLNLGHTVMSKRKL
                     HQLVEEKHVDGWGDPRLPTLKGLRRRGYTPEAIRNFCERVGVAKRDSVIEMALLEHAI
                     REDLNRSAPRVMAVLHPLKVIIENYPVGKVEALEAVNNPEDPTQGKREIAFSRELYIE
                     QDDFRENPSPKFFRLSPGQEVRLRYAYIIRCVGVEKDPETGEVTALRCTFDPETKSGG
                     NQSSRKVKGTLHWVSAQHALPAEARLYEALLTHTNPGSGGETQDLHRLLNPHSLTVLS
                     NCQVESGLRGAPTGSRFQFERQGYFCLDPDSTQERLVFNRTVPLRDTWAKIDKK"
     gene            complement(8058..8735)
                     /locus_tag="PKM72_06895"
     CDS             complement(8058..8735)
                     /locus_tag="PKM72_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60550.1"
                     /translation="MAKPVGKLRKINLSEVWGNEADGFASWLQQEEILDILGETIERA
                     LKPAGSDIPLSILSGGVLAKDAKTGSYVIVLGHLEGIDPDALGKLIMYAAGLDAKTVV
                     CVAQAISPEIRQTLDWLNGVSRDEVKFYGAELELWRIDDSVPAPNFHLVCQPNLWARQ
                     LKNGQDEGGESKAAKGSEFSELNAHNEEASKGTKEKGAAPQGKTAPVGKDGVSVRQNF
                     VYTKTFS"
     gene            9189..9455
                     /locus_tag="PKM72_06900"
     CDS             9189..9455
                     /locus_tag="PKM72_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60551.1"
                     /translation="MNRFPLLGLSLVCAFGLVASLSGCGETEGPAEKAGKNLDQAAEK
                     TSESVNSAMESTGEAMDNAAEQTGEAAHEAMESTEHMAEDAAKH"
     gene            complement(10374..10916)
                     /locus_tag="PKM72_06905"
     CDS             complement(10374..10916)
                     /locus_tag="PKM72_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60552.1"
                     /translation="MKKPKSLQSSGSRETFLIFIVVACGIMATLSVNLFNKSASDPSE
                     ANIGESTRSDQPRSVPEKVPSVKLTDVPLATGTEPMEKLFVQSGCAVCHTIPGIAPAL
                     GREGPRLVLGTNGPLRLADPQYQGTATTVREYIQESILNPGAYVVPGYSDRVMPRWYG
                     TRLNALALNRIAEYLENVKE"
     gene            11301..12191
                     /locus_tag="PKM72_06910"
     CDS             11301..12191
                     /locus_tag="PKM72_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNP60553.1"
                     /translation="MPTQERIFQVSPQVGLLTKCHWQPSPSQHPTILIVHGLEGCTES
                     HYMRGLARKCWDTGWNSIRINQRNCGGSEHLTPTLYHNGLSQDYGKIIQEITEEDGCT
                     AVWLVGYSMGGNLTLKLAGEHGTTLPSLRGVAAVCPNIQPAACVRALQRPSNWLYHRY
                     FLKSLTAKLRKKSQLFPGRWDLSHLSHIRTMWEFDEIYTAQDGGYRDAADYYEQSAAR
                     NTLSSIKIPTLVITAQDDPFIPYPMFADPALHSNPFIQLEAPAHGGHCGFFQRHHRQE
                     DPFWAENRICDWIQSQLALA"
     gene            12414..13766
                     /locus_tag="PKM72_06915"
     CDS             12414..13766
                     /locus_tag="PKM72_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HNP60554.1"
                     /translation="MHKAQERYLEGWLGVPAHVHHTAYRMANPASERPQSRKEFQQLL
                     NCLEIESSHVVLEDKVGFGVKKGTNGDKLIAIWEAHTEYYSYQIWHIPSDATQPLGFG
                     PLGFPGYVFPFSPLGIQVNALDLLVISARTYDQEELGTLLPGPQMYASKVLGEDICVA
                     TSFTPDEHDRERYLIWAPDPVLLRQKLARLITILTTLENYTHLILLPYPAFSRSVDQV
                     QIFEQRQLYQRTLITKELNLATHSTLQQWLEALTHDFLEVGRLTASLRYRLSASVPYD
                     RIVHSNTLALQEHPLPYGRTLIDYFQWKITGVSDGYQQLLTRIQALEQDFEGTIAVLR
                     TKVELLLQEQNLAQQDQNVKLLASLDKTTKSQVVLQHTVEGLSVIVIAYYVSGLANYI
                     FKALHELGWLDKYELASGIFVPIALGLSFAIITWGRNRIHKKISATTQPSVSHRPDRS
                     "
     gene            14020..14886
                     /locus_tag="PKM72_06920"
     CDS             14020..14886
                     /locus_tag="PKM72_06920"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP60555.1"
                     /translation="MLTAEELVKNCTKLFTLPDVYLQVKKVIDNPESTMADLSRAISI
                     DPGMTVAVLKLVNSAFYAMPRKVETISRAVGILGMQPLHDLTLAISVTQTFSGLNQQV
                     MSMSVFWSNSFFSGLVARELARKCFLVDSERMFVEGLLRDIGHLILYEQLPEPSEQVL
                     RESASTGTPIHIMEQRILGFDFTEVGQTLIEEWQLPKNFGIAIRYQNNPSGTTDHGFE
                     AALLNMASALTEGFQAPNGHAQWQHLVAPESWKLTGLDEDGLKECMTEAGKQLSGMLD
                     LMKEAHQPVAAG"
     gene            15395..15667
                     /locus_tag="PKM72_06925"
     CDS             15395..15667
                     /locus_tag="PKM72_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HNP60556.1"
                     /translation="MAGDMKATHSEVVMGLNGGDDLVWVRHGLVPIAQPAQELGYLID
                     HDFFVNGQFAMTEIVGKLTECHQAAQRFFKLWITDTLHQAMKPQIS"
BASE COUNT         4360 a         4321 c         3583 g         3817 t
ORIGIN      
        1 ccgggctcgc cttaagattt cggaggattg gacgcacaaa aataagaata aagccggcct
       61 cgaccatcgc cccggaggac atcgcgacag ttgccacgac aattccggga aaactggata
      121 cggctaatcc tccaagaaga acagcactac tcacaattaa tcggaccaac gttcccagac
      181 caatcaagcg ggtctggcct gcgcgaatga ccactccttg taaaaacctt ctcaccgcaa
      241 tggagagcgt ccaaggcgtc atgcactgga gacccattct ggcaggttcc acaataacct
      301 cgggaactcc caacacgtct tgtaccatga tcccatacac gggggtaaag gccaccacaa
      361 catgaatgac tgtcagaata cttcctaaga ccacaatgaa attgcgaata agccggtagg
      421 ctaaccaatc cttacataat gcggttgacg caaccagaat cataatgacg ggtgcttcga
      481 tcatgaggga gagaggaaaa accactccac cataagccgc taaatggatc tccggatcaa
      541 caaggcgtga aaccgtggcc gtcaccagtg gaaactcgag gcccatgagc atccaactcg
      601 tggccaaggg ccaccacatg cggaaaatcg tgtggtacgg agagaaaggt gtaaggagac
      661 cgggcgagtt agtagccacg aaacgacgag tgattcaaaa taaacatgtt cgctcagagt
      721 ccgacatggg caggaaagct gagaagaaac gaaatgcgaa gacgattata cacaactaca
      781 tatagctcat tgcctgggaa agtacagcag gaggtcattc acgaaaggga gcgacccttt
      841 cgtgaagatt ttacactatc atgaaagcga ggagttctgc caaaaggccg gaaccttaca
      901 acggagactg gggaagattg gtagaagaat caggcacacc tcgaatccca tgggcaacga
      961 attcctgttc ttcaggcgat tcccccgtga ccgactcggg atccgaagca aaacggcccg
     1021 catggctttc ctcctggaca tcctcttcct ggagatttac ttggtcaaca caagagggtt
     1081 tccatgtcga ggcttcaatt tcacattctt cccccggata gaaacgaagg gaatcccaat
     1141 gcgtcaccca actcttcacg tatttccgca tactggttcc tgggactatt tgggccggat
     1201 cattcgccgc attttcccac atatgccacc catgcccttt caggtgaagc gatcgaatcg
     1261 tctgataggc ttcttgaatg atgtcgttcg gtggctgtcc gaggggaata cgttgcgact
     1321 ccaataagcc catgcctttc tctgccttgt cggctaatat acgctcgtcc tctgtattct
     1381 tagctcgaag tttctccaag agtttaatct tgtcaccttt tttctctaag acctcttctc
     1441 gaaagtcatt ggttaatgcc agcacggtac ccaatcccgg cagagtagga ccgccatcca
     1501 aattcatgag gcgtgccact tcagcatagg atttcgcccg ctgattcacc gaatatcggc
     1561 cgatcaactc ggcttcatcg accagcaaaa tccacccggc ataccccgca gcttgcatca
     1621 gacgagccgc aaactgaaaa cgctgaagcg ccaactctgc agccgatatc ttgccaaacg
     1681 catatcgctc tccaaatccg gcctcttgca ggtacttttt taaatcgcct accccgatgg
     1741 ggtctcccgt ccaaaatcgg gtcattcggt gacccaactc catatcggag ctcaatcgtt
     1801 catacagcat caacgtcgcc gcaaacctcg aatcaatctg actctccgga ttattcaccc
     1861 acgtgacaag ctccttatag cggggtgccc agacatcaca ttgacccgcg atttccgaaa
     1921 acacttcccc tcgcttatca gggatcacgg cagcctcgat cgcggagtaa tacagacgga
     1981 cagggtgata cagaggcgtt tccttactga tcacaatcct gctgcagatg aaattggcat
     2041 ccaacgccag gttttggaga tactccagaa tatgcgactt cccactgcca aactcaccct
     2101 cgatcaccat gccacgaggg cacggacccg tcgtaatatt ttgctgtgta gcttctaata
     2161 accgccgaaa acgtccctcc acctcaggct gaagacatcc gagcgtcgtc accacatgcc
     2221 ggttcggaac cccagcccgc agaccttcaa tgacacgacg ggcttctcca tcaggtgcca
     2281 ttctcctagg catcccggtg gaagaaccgg tctctccttc agacaccgac tcatggtcat
     2341 cttgaatccg aaccagcgac gacaatggat tctggcggtg tttcggacga acgtcatccc
     2401 acactcgcag cttcaacgcc tcgtaccgat ccacgccccg gcgggcatcg gtcaaaaaat
     2461 cttgtggagc caggatcacc atcagcaagc cttccatctc atcagccgaa tcaataaatt
     2521 gacgaagcaa ttcatataaa tccatggtgg ctgaaggaga aaaggacaga gagccatcag
     2581 tcggcaacgc atccttttta ataaaatacc gggagatatc gagtgagaga aggaggcctc
     2641 cctgtcccgt taatcgaagc cagcgggtta aagaagacag catgtgccgt gcattatgac
     2701 gcgagatttt ttgatagatg agagcctttt tgactccagt cacaagccga aggtctccac
     2761 acagccactc tttcacggct gtggccaaga cacgatccga atcgcccgca tctaactgag
     2821 ccatgcacaa ccgaatcatc gccatgcgaa attcacggca caggcctgaa tcgccttcaa
     2881 ttgctttctc catccatgtt tgaatgtccc ggcggagcat cggctccttc cgttcattca
     2941 ccattgcaac ctgccgcaac gaaaattctt gccggtccga aggaatttga tgcccatgct
     3001 cttcgagcaa ccgttggaga aacctatagg caagttcatc ccaatctatt tgtttggcaa
     3061 ttttttgaaa cagtcgctcg accatgtggg cttttgtgaa tcgcgcgtcg actcttgcca
     3121 acacatatcc ttcacgtttg gcgagtccct ccaattcctg ttggcaggtt ctcaagacgt
     3181 cttcacccgg gagcacggca aatttgaccg cccctccccc ctcacggacg aaactcccaa
     3241 gatattcatg gtgaaggatg cccagccatt cgcggagatc cagattaaat cgtggaccct
     3301 cctcggggtg aatatttttc tttggcgatg gcttcgaatc tgactgatgt tgttgcacac
     3361 attcaaactc gtcctgacgt atgtcattga gcaaagaggg ggaggaaggc agctcctttt
     3421 ctgttctcac ggactgtaaa cgattcaagg cctcaattaa tttgctcatt gtgcggttcc
     3481 ttcccccatg aacattctga aaatttcaga tttcaccact ccacacctta ttccatgacc
     3541 atccttgacc atactttgac aggctccact aaacataaat cccttacaca ggaaaatgca
     3601 cactgatttg acaggcacgt ttaaacaatt gattggctcg tgcttcgcat atttcatcat
     3661 cgtgaagatt gaataaggtt cgtccgattt catgaaacgg cacacccaga tccaccgcat
     3721 ccaacacacg tagatacatt ggccattcat cacgacattt tcgccgttcg agacttcgac
     3781 ccacttgatg tttttgccat tccataagat ctgccttaac tttttcaatt tgacccgata
     3841 agggcttttt cagatcaaac acggccgcca ttcgccattc cgacaaacac aaggtgacct
     3901 cttcacctcc ccccagccaa tccggtccct gtccgaaata gatccgaccg taattcgaat
     3961 caaaggacaa catccagggt ttcgcaatgg agggattggg aagcccactg agatggtatt
     4021 tttctaatga attcggcatt cgagctttaa atgccggatg atccggcgaa aaaacttttt
     4081 tgcgataccg ggtgggatac tgaggatctt tggcacagga cagatcaaaa tcatatgttt
     4141 gtaggtaatg ttgttcccaa tccttccggt aatctgaacg ccgacggaga aactcccacc
     4201 gccaaaatgt atcgtttaaa ctctgagcgc agggataggt ctcctcaatc ttccagttgg
     4261 gaacggggac tgacactcgg ggatgcccgt gtatttcctt gggcggtcgt ttactgctag
     4321 acatcttgca ggacctcctg ttcgggaata ttcaatggta gaatgccgga tattaggtac
     4381 tttcgtgccg ggagaagaca gccaaagacc gtcagtcccg gctcacgtga cattccagcg
     4441 accaccctca aacccgcagg cactcaggta tactgataac ccggtttaaa aaatgatttt
     4501 gtcgattctc aaactacggc acgaataaaa gaggcaggtg tcgggagacg aactgaggga
     4561 ttttcattca tgatgaggag aacaggtttt gcgccttgac caagagaccc catggaccca
     4621 tccaaaccag gagagcaccg tcacgaaaca agctaaagca accatggcca agacaaaaca
     4681 aattttccct aacatgtcga agacccccag gatagcggtt cttcatgcct gccaatactc
     4741 tctgtatcgg cagagttttt acaggtcttt actagaaacc aaaaaaaaca tacctttctt
     4801 taatgaaaat aactccaaaa attctacaaa acaatgggca agactttcca ttccttaatt
     4861 ttcatctatt tttcctgtaa atcaccaaaa tccagaatac gtttataaaa aatggatcac
     4921 aggcaattgg tacattcctc atcagttcag acgggatcgg atcagaaaac caaaaacgat
     4981 tgatgaaaat atttttctta aaaaatcaat atgttagaac aaaaataggc agaacatgtg
     5041 agctccctag tacaagaact atcaaagatc tcgaattact tcttagctgt cgattccaac
     5101 aaaaaaccaa ccatgtctga ctttgagagg tcgatcacag aagcacagac tcctcgtccg
     5161 gcaccgacca ccgtcatcct tttccataag cccaaagggt atctggtcac tcgaaaggat
     5221 gagagggaga gaaaaacggt ctatcatgtc ctcccgcctt tcctattgca ggaaggatgg
     5281 gtacccatcg gccgactgga caaagactcc cgtggcctcc tgctcttcac ccaaaatgga
     5341 acaatggttg accgcctcac tgagccaggc ggatgcgaaa aaacttatgc gattgagatc
     5401 aggggaagaa tttcagacaa tgacctatcc cgggtgttac agggagtttc cacttccatc
     5461 ggaccattaa aagtacacag gattaccaaa aaaagagaag tcggacccaa aacacaacta
     5521 gaggtcgtct tacgggaagg gaaaaaccgt catctccgtc gaatctttca tgccctaaag
     5581 gatccaaaat ttcatacgcc gctcaaagtt ctcgacctca aacgaataca aatcggccca
     5641 ttgaccttgg atatcccggt aggggcatgg agattttcaa caccggacga ggagactcaa
     5701 ttgctggcta tttttaaaat ccccatacat cctggttaaa ttcctgctca agaagctttc
     5761 atttcttatc aattttcgcc caggtatctc gaagaggcac cgtccggttg aacactaacc
     5821 gttcctgagt agagtccgga tccaggcaaa aatatccctg ccgctcaaac tgaaaccgac
     5881 tccctgttgg cgctcctctg agaccggatt cgacctggca attagacagt accgtcaagg
     5941 aatgtggatt gagcaaccgg tgaaggtcct gagtctcacc tcctgaaccg ggatttgtgt
     6001 gagtcagcaa ggcctcatac agtcgcgctt cggcaggaag ggcatgctga gctgaaaccc
     6061 agtggagagt cccttttacc tttcgacttg attgatttcc accacttttc gtctcaggat
     6121 caaaagtaca tcgtagagcc gtgacttccc ctgtttctgg atctttctca acaccgacac
     6181 agcgaataat atacgcatag cgaaggcgta cttcctgccc tggagataag cgaaaaaact
     6241 ttggagacgg attttcgcgg aaatcgtctt gctcaatata cagttcacga gaaaaagcta
     6301 tttcccgttt gccttgggtg ggatcctcag gattattgac ggcctctaaa gcctcaacct
     6361 tcccaaccgg ataattttca ataatgactt tcagtggatg caagaccgcc atcacccgtg
     6421 gcgccgaccg atttaaatct tcccgaatgg catgctccaa cagggccatt tcaatcacac
     6481 tgtcacgttt cgccaccccc acacgctcac aaaaatttcg aatcgcctca ggtgtatagc
     6541 cccgacgccg aagccctttc aaggtgggca acctgggatc cccccacccg tccacatgct
     6601 tctcttccac caactggtga agcttgcgct tactcatcac ggtatgcccg aggttcaaac
     6661 gggcgaattc aatttgctgg ggatagcacg agacttcaca tacctggagg agccaatcat
     6721 ataagggtcg gtgatcctca aactccaggg tacataacga atgagtaatg ccctccaagg
     6781 aatcggaaag cggatgcgca aaatcatagg taggatagat gcaccagtca tcacctgtcc
     6841 gatagtgcgt ggcatgtcgg atacgataca aggcaggatc acgcagatta atatttggag
     6901 aggtcatatc aattttggcc cgcaacacat acaccccatc cccaaattct ccggcccgca
     6961 tccgagtaaa taaggccaaa ttttcttcaa tggtgcggtt ccggtgtggg ctatccttcc
     7021 ctggttccgt cagagtcccg cgataaaccc ggatctcttc ggccgttaaa ctatcaacat
     7081 aggcgtggcc ggtttgaatc agtcgcaccg caaattgata gagctgctca aaatagtctg
     7141 aggcaaaaaa gaggcgatcg ccccaatcaa agcctaacca ccggacatct tcctgaattg
     7201 aggagacata atccatgttc tctttggtcg gattcgtatc atcgaaacga agattacaca
     7261 gaccaccgaa ttcctgggca atgccaaaat tcaggcaaat cgatttcgca tggccaatat
     7321 gcaaatgtcc attgggctcg gggggaaaac gggtatgcac ttggtgggga atagcctggc
     7381 gttccaggtc tgaagaaacc tttgctctga taaagtcgat ggaagaatgt tggtcaagtg
     7441 acgacataat tacaagcttt ccgaaaaatt aatgccagaa tttcaaagta atcgcatcta
     7501 caggaacaaa acccggtttc tttggtttcc atcaatcagc acatagaaac tcgtgaaagg
     7561 atgatccagg cctctcccaa tacctctttt gcaacctgtg tgttgttttc cgttcagaag
     7621 accgatcccc cacgctacca tgagccacgc caactctcaa tacgggggag acaaaatccg
     7681 atcccttttg attacccaaa caaggcaggt aagacaggaa cgtcgaaact ctcgggaaaa
     7741 attcgcaagg ctggtgaaga gacaaagaca atccgattca gcctatcccc cgttttgaat
     7801 ctcccgcaag aatgaggaat agtcggttta ttaagcaggg aaaaccctga ctttatggac
     7861 agatggcaaa ccaacaagaa aaaaattggg agatttctac agtgaattac aaaaaaatcg
     7921 ggcattggga gaggagggag cgacggggaa ggaggagcgt ttttacgcca tccaaaagac
     7981 ccagtccctg tgatatccgt ggggggtggt agaagattag ggtaaccacg gactggcgga
     8041 aggaccggaa acttgtttta ggagaaggtt ttggtataga caaaattctg acgcacggag
     8101 accccatctt tccctactgg agccgtcttt ccttgtggag cggccccttt ctcttttgtc
     8161 cctttcgagg cttcttcatt gtgcgcgttc aattcggaga actcagatcc ctttgccgct
     8221 ttcgattccc caccctcatc ctgaccattt ttcaattgtc tggcccacag gttgggctga
     8281 caaacaaggt gaaagttggg agcagggacg gaatcatcaa tgcgccataa ttcgagttca
     8341 gccccataga atttcacttc atcacgggaa actccattca gccaatccag ggtctggcga
     8401 atttccggag aaatggcctg ggccacacac accaccgtct tcgcatcaag gcccgcagca
     8461 tacatgatca gtttacccag ggcgtcggga tcaatgccct ccaaatgacc caacacaatc
     8521 acgtaactcc cggtcttcgc atctttggcg aggacgcctc cggacaaaat actgagagga
     8581 atgtcacttc cagcgggctt gagggctcgc tcgatcgtct cacccaatat atctaaaatt
     8641 tcttcttgtt gaagccacga ggcaaacccg tcggcctcat ttccccacac ttcacttaaa
     8701 tttatttttc gaagttttcc aactggtttt gccataacaa cctcaaccgg ataattgttg
     8761 gtttttgctt actattccaa tgagacgtac tagttcttat cgaccgttgc tcaaaaaagt
     8821 taagacaaaa aacacgaaac gcacgacctt cctatgaaca gagtcatctc gcgtcatacc
     8881 gacagccaac ccaaacattc ctcaggaagt cctcatccaa aacgcccagt ggcttgaatc
     8941 tgaaccatcc ctgaagtttt gggtttcaat caggtctccg gtcataggag acattcacgt
     9001 aaaacaaatt acttatgact cattgatctt tcttcaattt agagtacaat agattaagtt
     9061 cattcacgca tgaatggtcc tcgtattcca ttcaacaccc ttctcctgac cctaagatcc
     9121 cactggaacg agagaccaaa taggatggac aagcacgtgt ttcccacttt aaacaggagg
     9181 tttctgctat gaatcgattc cccttattag ggctaagtct tgtttgtgca tttggactgg
     9241 tggcttcact atcagggtgc ggggaaacag agggccctgc cgaaaaggct ggaaagaatc
     9301 ttgatcaagc ggccgaaaaa acatccgaat ccgtcaatag cgccatggaa agcacgggtg
     9361 aagccatgga caatgctgcc gagcaaacgg gagaagccgc ccacgaagca atggaaagta
     9421 ccgaacatat ggcagaggat gccgcgaaac attaaggagg ctttccttta ttcacctcgc
     9481 tgctagtacc tgccaccctc ccacagtttc cagcttgaac tgccaacggc aggtagggga
     9541 ggggtaaaag atctcatctt cctctttttc ttgtgcattc agatggtgaa tgtcatacca
     9601 tcagaatcca gggaagaggg ggggtaggtt ttgttgtgaa cacaaacctg agctaagacg
     9661 aaaacttcaa cacggatgct caagggataa ttcagaaata atgcggagtt gagtcccact
     9721 gctcacatta cgtgtttaac caaaaggggg caagaacgac atgatggtcg ataagtggat
     9781 aaaaactatc gtggccctgc tagctctcct ctttgctctc tctagtacgt ccctacgggc
     9841 agaaaaccag ttggagtttg gaacggatac cgggttcctg gcaggtaccc accgatgata
     9901 ccgtgttcac gctcagttgc caggtacact attttctggg ctcataattc tcggtcggcc
     9961 cacaactcct gatttcccct gccgaaggcc tcactcagat tacctccact tgaatcggcc
    10021 ggtatcacat tccagtcgtt ccagtcacca tcattccttt tggcggagtg gagcttgttt
    10081 atgcgaatca tgaccaagtc agtcaggcta tgtccctggc aaccacgctg attttcacct
    10141 tccaggatct gtgtctaccg aatcaggtga gtagcaacca ccatcggtat gttattggga
    10201 attcgattct aactctaata ttcgcaaagc ccaacctccc tacgatgcca ggcattccta
    10261 gggttagatc accatcaaca tggaaactta tgttctccaa ggtgttggga caaagtaaga
    10321 cgacatgaga caaactgacg cattcacgtc gtttgaagct gtccgggaat tttttactcc
    10381 ttcacatttt ctaaatactc tgcaatacga ttcaatgcca gcgcattgag ccgcgtccca
    10441 taccaccggg gcatcacccg atcggagtat cctggcacca catacgcgcc tggattcaaa
    10501 atggattcct ggatgtattc tcgaacggtt gtcgccgtcc cttgatattg agggtctgcc
    10561 aatcgaaggg gtccattcgt ccctaagact aaccgaggtc cctcacgccc cagagccggg
    10621 gcaatacctg gaatggtatg acacaccgca cagccggatt gcacaaacaa cttttccatc
    10681 ggctcggtgc ccgtggccag tggcacatcg gtgagtttca cagatgggac tttctctgga
    10741 acagaccgtg gttggtccga acgggtggat tcccctatat tcgcttcgct agggtccgat
    10801 gccgatttat tgaacagatt cactgaaaga gtcgccataa tcccgcaggc cactacaata
    10861 aaaatgagaa acgtttcccg gcttcctgag gattgcaagg actttggttt tttcatagac
    10921 acttccacaa tcctacggta aagattgaca gcatacggcc aaacaagatg ggccaacaac
    10981 gaataaaaac ctgaggtggt ctaccgaaag ttccactgtc ctgtcaaata ggtactcccc
    11041 gttcaatgtg atcacaccta cgtgattgac cattcaatac tgatccattt cctttatcca
    11101 gacttgtttc acacacagga gttttggtat cccaatcatg agagacagcc aaagagccct
    11161 caccgtcaac ctgatcccca cacatgctta atcattcctt gtggccatcc aaagcattta
    11221 ttccacaccc gtggttagct gacccacacc gtatgaccct tttcccacga tggtggccac
    11281 gcggtagttt tccatccgga atgcccacac aagaacgcat ttttcaggtc tccccacagg
    11341 tgggccttct gacaaaatgt cattggcaac cctccccctc acaacacccc accatcctga
    11401 tagttcatgg cctcgaaggg tgtaccgaat cacactatat gaggggtctg gccaggaaat
    11461 gttgggatac aggatggaat agcattcgca tcaatcaacg aaattgtgga ggatctgaac
    11521 atctcactcc cactctctat cacaatgggc tcagccagga ttacggcaaa attattcaag
    11581 aaattactga agaggatggc tgcacggcag tgtggctcgt cgggtattcc atgggaggca
    11641 atctcacact caaattagcc ggcgagcatg gaaccaccct cccctccctt cgtggggtcg
    11701 cagccgtctg tcccaacatc caacccgcag cctgcgtacg cgcactgcaa cgtccctcca
    11761 attggctcta tcatcgctat tttctcaaaa gcttaaccgc caaattacga aaaaaatcac
    11821 aactattccc gggtcggtgg gatctctcac acctctctca tattcgaact atgtgggaat
    11881 ttgacgagat ttataccgct caggacggag ggtatcgaga tgccgcagac tattacgaac
    11941 aaagcgccgc tcgtaatacg ctgtcttcaa tcaaaattcc gaccctggtt atcaccgccc
    12001 aagacgaccc gtttatcccc taccccatgt ttgccgaccc tgctctccac tccaatcctt
    12061 ttatccagct tgaggcgcct gcacatgggg gtcattgcgg gttttttcaa cggcaccata
    12121 ggcaggagga ccctttctgg gcggaaaatc ggatatgtga ctggattcag tctcaactcg
    12181 ccttagcatg atccccatcc gttcctgggc catccccttt tgaacacaat ccaaaccccc
    12241 ttcaaacact caactgcctt cattcctatt ctcctcttgc ctgaaaccta tcaggtaggg
    12301 tagaactctg ttcaaatgag taacctttcc ttccagtttc gattccaatc acaagtcttc
    12361 gcgcaccaat gaaattaaaa cccggttccc caggaaaagg aattcttcgc caaatgcata
    12421 aggcgcagga aagatatttg gaaggctggt tgggtgttcc ggcccatgtg catcacacag
    12481 cgtatcgcat ggcgaatccg gcaagtgaac gaccccaaag ccgaaaagag ttccaacaac
    12541 tgctgaactg tttggagatc gaatcttctc acgttgtcct ggaagataag gttggattcg
    12601 gagtcaagaa aggaacgaat ggggacaaac tcattgccat ttgggaagcc cacacagaat
    12661 attacagcta ccagatttgg catatcccct ctgacgcaac ccaaccatta ggattcggtc
    12721 cgttgggatt tcccggatat gtgtttccct tttctccgtt gggcatccag gtgaacgcgt
    12781 tggatctgtt ggtcatctcg gcgcgtacct acgaccagga ggaattgggg acccttctcc
    12841 cagggccgca aatgtatgcc agcaaggttc tgggggagga catctgcgtt gctaccagct
    12901 ttactccgga cgaacatgat cgggaacgat atctcatttg ggctcccgat cctgttttac
    12961 tacgccaaaa actggccaga ctgattacaa ttttgaccac acttgaaaac tatacccatc
    13021 tgattctcct tccctatcca gccttctccc ggtcggtcga tcaggttcaa atcttcgaac
    13081 agcgtcagct ctatcaacgg acccttatta cgaaagaact gaacctggcg acacatagta
    13141 cccttcaaca gtggctggaa gcgttaaccc atgatttcct tgaagtgggg agattgaccg
    13201 cttcgctacg ttatcgcctt tcggcttcgg tcccttacga tcggattgtg cacagtaata
    13261 ccctggcact tcaggaacat cctctgcctt acggtcgaac gctcatcgat tattttcaat
    13321 ggaaaattac aggggtgtcc gatggatatc agcaattact gacccgcatt caggccttag
    13381 agcaggactt tgagggcacc attgcggtcc tgcgcactaa ggtggaacta cttttacaag
    13441 agcaaaacct ggcgcaacaa gatcaaaacg tgaagctcct ggccagcttg gataagacca
    13501 cgaaaagcca agtcgtctta caacacaccg tagagggcct ctccgtgatc gtgattgcct
    13561 attatgtaag tggcctggcc aactatatct ttaaggccct ccacgaactc ggttggctgg
    13621 acaagtatga attagcctct gggatttttg tgcccattgc cttgggcctc tcatttgcca
    13681 tcatcacatg gggcagaaat cgcatccata aaaaaatatc agcaacgacc caaccctccg
    13741 tctctcatcg ccccgatcgt tcctaattgt tacgcccatt ctcctcgttc cttcgccagc
    13801 atgaatgaag cctgacctct cagggcagcg acagactcct tcctgccttc tgaacggaca
    13861 tcccatttga aaaccgacct caaccagctg catgaaccaa cagccaaacc ggaactgaaa
    13921 agaattcatt ttaagtaccc cagggaaatg tccgagaact acaagaaaga ggcaaatttt
    13981 tggccattga acaccagagc tgcaccagaa gggatcaaca tgctgacagc cgaagaactc
    14041 gtaaaaaatt gcacgaaact ttttacgctg ccggacgtct atctgcaagt caaaaaggtc
    14101 attgataatc ctgagtccac catggccgat ctgtccaggg cgattagcat tgatcctggc
    14161 atgactgtcg ccgtcctcaa gttagtcaat agcgcgtttt acgcaatgcc tcgaaaagtc
    14221 gagaccattt cccgggctgt cggaatcctc ggtatgcaac ccctccatga tttgacgctc
    14281 gccatttcgg tcacccaaac cttttcgggc ctcaaccaac aagtgatgag tatgagcgta
    14341 ttttggtcaa acagcttctt tagtggactg gtcgcacggg aattagcaag aaagtgtttt
    14401 ctggtggaca gtgaacggat gtttgtagag ggtttgttaa gagatatcgg acatctcatc
    14461 ctctatgagc aacttcccga accatcggaa caggtgttac gagagtccgc ttcgaccggg
    14521 acgcccattc atattatgga acaacggata ttgggatttg attttacgga agtgggccag
    14581 acactcattg aggaatggca actaccaaaa aatttcggga tcgccattcg gtatcagaac
    14641 aatccctccg gcaccaccga tcacggattt gaggccgcgc tcctcaatat ggcgagcgca
    14701 ctcacggaag gattccaggc acccaatggg catgcccaat ggcaacacct ggtggctccc
    14761 gaatcgtgga aattaacggg tttggatgag gatggcctca aagaatgcat gacagaagca
    14821 ggaaagcagc tctccggtat gttagatctc atgaaggaag cacatcaacc cgtagcagca
    14881 ggataatggc aaggaagccc tgcatggcag aagattacaa gaaggaaaac cgaacacggg
    14941 aattactcat caattcaaaa aggcaatgcc ctttgccaac aaaaactcac ccgtccatca
    15001 tgtatcgaaa gacacccatg ctcgatgtca tttcccaaat gtccttagct cctccggtca
    15061 cttttcagga atgaggcaca atcctctttc caaattttcc cctgacacaa gatcaagctt
    15121 cgtataattt ccccagtgcc gttcagctgt ggcaatggcc tttaaatcgg ggatctcttg
    15181 ccgtggttgc caggccgtac aagggatggg gagcctttca agcacatatc aggctggcga
    15241 cggacgcagt agagatcgaa taacccccac cctacgtctc ccgtatcggt taagtttcag
    15301 agatatcatc cgaaggtcag tggttggcct cacgaacata aattgagatc tactcttaca
    15361 caataaatag acaggagatc tcgcctggtc tggggtggct ggagacatga aagccacgca
    15421 tagcgaagtg gtgatggggt tgaatggtgg agatgacctg gtgtgggtgc gacatggact
    15481 cgttccaatc gcacaacctg cgcaagaatt gggctatctc atcgatcatg atttctttgt
    15541 gaatggacaa tttgcaatga cagagatcgt agggaaactg acagaatgtc accaagctgc
    15601 ccaacgattc ttcaaattat ggatcaccga tacgttgcat caggccatga aaccgcagat
    15661 ctcctgaagg aagacgccca tctgctgaac aggctcaaaa gaaaataaac cacacgtcat
    15721 acgaattgct ttatttcatt aacgcgcgat ttcatataaa ggagtgatca aaaaaggttt
    15781 gtccagcata gccgtagccg cttggactaa tgaagcacac gaaggaatat acgagcagga
    15841 ccaagtgtcg agaacgccac tggcggcatc attcaacact ccctgtttgg aaagcgattc
    15901 cctccccagc tcccgctagg atactgagtg actcatcaat ttctcatctt gagctgacgc
    15961 agcaaccgct tcattaacca tccactgttc cacccgattc cgcccattct ctttggccac
    16021 atataaggct ttgtcggctt gggccaccaa ttccaaataa tcagaagctc ctaaagcggc
    16081 g
//
LOCUS       DAORAQ010000055        15680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_638_length_15680_cov_66.139200, whole genome shotgun sequence.
ACCESSION   DAORAQ010000055 DAORAQ010000000
VERSION     DAORAQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15680
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_15680_cov_66.139200"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKM72_06930"
     CDS             <1..308
                     /locus_tag="PKM72_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HNP60557.1"
                     /translation="KPAAACLSSRITRGLPITKAYLSRVEQAEAFLVAQGITQVRVRL
                     HGDLARIEIDPTQMGILMEEPGRSRLVEKFKKLGFQTITLDLEGYRAGGGNLPSGPV"
     gene            complement(504..1010)
                     /gene="atpF"
                     /locus_tag="PKM72_06935"
     CDS             complement(504..1010)
                     /gene="atpF"
                     /locus_tag="PKM72_06935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNP60558.1"
                     /translation="MPQFDSHFFSSLIFWELLSFGILLWVLYKYALPPILETLEARER
                     KIRESLDQAEQNRVAAEGKLKEYETKLQLAAKEVETILTEAKQQAQRLLEENEQRLRV
                     ESQRIKEETTQDIDRERRKAIQDIKTQSAELAILVAEKVIGRSLSDDDHRRFAQEALE
                     AVAAQSKN"
     gene            complement(1025..1252)
                     /gene="atpE"
                     /locus_tag="PKM72_06940"
     CDS             complement(1025..1252)
                     /gene="atpE"
                     /locus_tag="PKM72_06940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNP60559.1"
                     /translation="MDSAAAALLGMGLAAAGFAGAGIGIGYIFGKMIEAVARQPEAEA
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGKG"
     gene            complement(1324..2049)
                     /locus_tag="PKM72_06945"
     CDS             complement(1324..2049)
                     /locus_tag="PKM72_06945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNP60560.1"
                     /translation="MEDPLHPFELHNFLPLSFFGLDISVNKAVLMMWVVVGLVTLLLM
                     KAGGARALVPSKLQSLGELLVDFIRGIIQDTMGASGMRYFPLISALFLFILFSNLVGL
                     IPGSYTITSQIIVTGVFAVGVYLLSLIIGFQLHGAKFFGILVPPGTPGWLLPLMIPIE
                     LISQLARPISLAVRLFANMTAGHVILGVLFGLTIAGGLMIGWLPFSFTIALYGLEVGI
                     AFIQAYIFTVLTCVYIGDAMHLH"
     gene            complement(2073..2354)
                     /locus_tag="PKM72_06950"
     CDS             complement(2073..2354)
                     /locus_tag="PKM72_06950"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNP60561.1"
                     /translation="MIRDFLLECVPFRRLAWPIKKMAPSQDPMFAGLGQAMRVGTDLL
                     AALIVGGFLGWLIDSYVLDTTPWGVALGLVLGLIAGVRNAYRAAQRWKQ"
     gene            2474..3058
                     /locus_tag="PKM72_06955"
     CDS             2474..3058
                     /locus_tag="PKM72_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60562.1"
                     /translation="MMKKQLVFLLIMAFAAYENPLGATSSGVDDLPDLTGGVVPVVQY
                     KQLDPFSGNDILTSVSEVEYTVKVKNQTGDPLIADSLILVVDSVLEISGKEISNRIKI
                     EGSDGMTGEGKHFFRIPSNRKDLPPYGESETVTIRVTNPDFLRFYPPGVRVRGIRRSS
                     EKAAKDLLDNLVQEGLLSPEDAMRALENPSSDSP"
     gene            3062..4570
                     /locus_tag="PKM72_06960"
     CDS             3062..4570
                     /locus_tag="PKM72_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNP60563.1"
                     /translation="MSSIPKHAERGTHASLHSTSCFPLIQHCSPAFEDPFDLFGRVTG
                     YAPHSFFMEHDAVQDGVSCRFSYLGCDPYRVVRGKGHAYESVSLGKKEKHIGDPFAML
                     QRTFARQTAGSGHQFSPFQGGAIGCFSYDLARTFEVLPECLPDDLHFPDLYFLFVEIF
                     VAVDHQAPGVWLIFAPSPERLAGESWDHLLREGQARLSDLQAKVMIPENLQTKMHSAM
                     SSLRIEGEQSSLEYMDRVRACKHFIAAGDIYQANLSHRFRVEGVTHGFASQVEAGADI
                     YRQLRKVNPSPHSAFLVLESDVIVCNSPERLVRLSGGRTDMRPIAGTRSRDIEPQVDR
                     RLAEDLLSCPKERAEHLMLVDLARNDLGRVCDYGSVRVNELMTVERYSHVMHMVSQVS
                     GRLSDVCHGFDLIRATFPGGTITGVPKVHCMELIEQLEPVRRGIYTGSIGFIGWNGDL
                     DLNIAIRTLWLTAGQGYLQVGAGIVADSDPEREYKETLKKAEAFFQVLKGNY"
     gene            4591..5472
                     /locus_tag="PKM72_06965"
     CDS             4591..5472
                     /locus_tag="PKM72_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNP60564.1"
                     /translation="MWIFLNDQFVKKEDARISVFDHGFLYGDGVYETLRVYQGRIFLL
                     ERHLARLRRSCDLIGLVLPIQDDAWEAIMTEMLIRNELEDASLRITISRGEGELGIDP
                     GLCPRPTIVVMARSVVAYPAQMREQGVRVQLVSVRRNPESAQSPQIKSLSFLNNILAK
                     QEAVQARAFDALMLNMDGHVTECTTSNIFFVANHRLHTPSVACGILEGITREVVIALA
                     RDLKIDVEEGAYSPMDMLQADECFMTNTGLEVMAVSQIGEDTIGQGKSGPITMVLWKG
                     FQDNLERWLGPVITRPQ"
     gene            complement(5482..7131)
                     /gene="nadB"
                     /locus_tag="PKM72_06970"
     CDS             complement(5482..7131)
                     /gene="nadB"
                     /locus_tag="PKM72_06970"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNP60565.1"
                     /translation="MSSAAASHHSSHVISTDFLILGAGVAGLRAAIELSRYGRVTMVA
                     KGGPQDNNSFYAQGGVAVALSEEDDVVLHLADTLKAGHQLCSRPATKILVEEGPSRIH
                     ELIEWGAKFDTVDGKLAFTREGAHSRHRVLRAGGDATGSEMVRALSVKAQTLKNLTWM
                     GNHVAVELCIQDGRCWGALILDELSGTLKIVCAPATLLVTGGAGQVYARTTNPANATG
                     DGIAMAYRAGAILEDMEFVQFHPTALYLPSSPPFLLSETLRGEGGILRNNRCERFMKN
                     YHRSQDLAPRDIVSRAIWTEMQRTKARHVYLDVTHLGAAFLKERFPTIYSTCLRYDID
                     ITEEWIPVSPSAHYFMGGVKTDLDGASTIPGLFAAGEVACSGVHGANRLASNSLLEGL
                     VFGYRAAQAASSCSTISSCPDFSNSPLLRKPSGRKMSTQDVEKIRNSLRRLMWSKVGL
                     VRTGNSLKKTVDQAQLWSSKLSAAPWSRPGLETRNMVLVGQCIAEAALWRSNSVGAHF
                     REDFPFHKGLAWKTHSRCQQDNGAKPTAVITPKNTKNTTPR"
     gene            complement(7164..7658)
                     /locus_tag="PKM72_06975"
     CDS             complement(7164..7658)
                     /locus_tag="PKM72_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60566.1"
                     /translation="MILLRFLNNHLLLLLITFPILISLAGSGCSKKAPKYPEDHARFQ
                     RVVQAIKTLEKAYVNHDASGFQDLLLPLENLDLLEAKVREDFATFTTIHLDLTIDRMV
                     IDGDRISAFVSWQGNWERSPQEAPAQASGHGVLLWSGNQVILLRGIEGNLPFAMTHRL
                     NFSS"
     gene            7882..7977
                     /locus_tag="PKM72_06980"
     CDS             7882..7977
                     /locus_tag="PKM72_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HNP60567.1"
                     /translation="MSSVVKKRRKKMRKHKHKKLLRRMKFAKRRN"
     gene            8010..8231
                     /locus_tag="PKM72_06985"
     CDS             8010..8231
                     /locus_tag="PKM72_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60568.1"
                     /translation="MTQGKKTRIRVRSSLSSYVGDLLIPPMRNRISDVLNDESVLFIN
                     LTDVLINDTEHSAFVALNKNQIESVIQLD"
     gene            8339..10105
                     /locus_tag="PKM72_06990"
     CDS             8339..10105
                     /locus_tag="PKM72_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP60569.1"
                     /translation="MNYLSRFIPFLRPYLMTMVGAGVLVMGVAACNLLLIRLGGSLWD
                     LITVQRDLPKLTSMVWIFVGLVIGQGLLSMAHSYLTALASQHVMADFRTHVFSHLHRL
                     SLNFFAKRRTGELISRLMNDVGVIQNLLTETPMDALKHLVTIIGGVGFLLVMNWRLCL
                     LILILLPLLALVARMFGKRLKVLSMQIQDQTAHVTTLIEEVVSGIRVVKSFVQGKREE
                     TRFRSAVETLLATTMKRTAVLAVFVPVITFCTFVMAIGVLWYGGKQVIEGQLSPGELF
                     AFVLFAGILIGPFGSAARMFSQVKEVQGAMTRVFELLDTPLEIQDSPMAKALTPIHGQ
                     VQFDHVQFSYEGRPPVLDAVSFSIQAGECVALVGPTGAGKTTIVNLLHRFYDPTAGRV
                     LVDGHDLTSIQVDSLYQQLALVPQETLLFGGTIADNIRYGRAEASESDMIEASRRANA
                     HEFIQALPEGYDTILGEKGINLSGGQRQRIAIARALLKDPRILILDEATSALDSQSES
                     LVQSALTELMKGRTTLMIAHRFSSIQRADRILVLHKGTIVEEGQHEELLAKRGLYHHL
                     YTLREVEGITERMEIAEQPLPQ"
     gene            complement(10223..10888)
                     /locus_tag="PKM72_06995"
     CDS             complement(10223..10888)
                     /locus_tag="PKM72_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60570.1"
                     /translation="MVSLLSSYIRLPLTLCALTIIGVGTHPSISSGIDLSLTLEQANE
                     AMASGRKTMEQADSVEDVATVMKASERAVRVGANPEVEPCGAHAILRTRLYWLEYFGR
                     REAAESKRQKQDIRMPEAKIQEILEMPYLEVEIRLCGEDEFFAEGADIALQQGSNTIR
                     PVDIGPPERGRKNPGDTVSFRSRFTARFAYADFDPLAPGTLAVFFPDGKLINIPADFS
                     KIH"
     gene            11248..11916
                     /locus_tag="PKM72_07000"
     CDS             11248..11916
                     /locus_tag="PKM72_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP60571.1"
                     /translation="MNIEKVERVYSAYASVYDHTFGKVFQQSRESAVRGLPIEPGHRV
                     LEVGVGTGEALPLYPSCCEVLGIDLSDGMLEHARRRVQEHRLDHVTLKRMDAGKMDLP
                     DNHYDIVMAAYVVTAVPDYRSVVDEMIRVCKPGGRIIMLNHFSNGNKVIAAVEKWISP
                     FCKHIGFRTDLALHTVLEGTCLMVVNKKKVNPLRFWHLVECVNKKNGQQALFTNGFAL
                     NGHS"
     gene            12058..12738
                     /locus_tag="PKM72_07005"
     CDS             12058..12738
                     /locus_tag="PKM72_07005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNP60572.1"
                     /translation="MKVALCLQHVLFEGPGVFRRALETRGYAVRKVVVPAEGLPADPW
                     DFLLIMGGPMSVNDGDAWVEAELQFVKTALAKDIPVLGICFGAQLLAKALGGSVTPGP
                     KFELGMSAVSLTDLGKVDPILSAMPQDFLVFQWHGEGITLPPGSVHLVTSADFPVQVF
                     RMADRTYGLLFHLELEELGIEALCRECPQDVQRGGMTPESIQAPSRPHLPRLHQLADR
                     FIEHLIQA"
     gene            12874..14049
                     /locus_tag="PKM72_07010"
     CDS             12874..14049
                     /locus_tag="PKM72_07010"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNP60573.1"
                     /translation="MAEFPIQYAERIRTLPPYLFAAIDDMKRKAIERGMDIINLGIGD
                     PDLPTPEPIIESLRRAAGNPKHHQYPSYDGMLSFRTAVADWYQRRFGVTLDPKTEVVT
                     LIGSKEGIGHVPLAFVDPGDIVLVPSPGYPVYPVATSFAGGISYTMPLTKENGFLPDL
                     SAIPKDIAQKAKMMFLNSPNNPTSVVASKEFFKKVVDFAREHQIIVCHDAAYSEIYYD
                     GNRPASFMEAAGAKDVGVEFHSLSKTYNMTGWRIGFAVGRKEVIAGLGKVKTNIDSGV
                     FQAIQEAGITALQLDGSVTEGLRTIYQERRDVLVPGLKAMGLELDSPPAAFYVWIGVP
                     KGFTSASFTAHLIEKAGIVTTPGNGFGDPGEGYIRMTVTTTKDKLAEAVERMKKIGW"
     gene            14087..14641
                     /gene="folK"
                     /locus_tag="PKM72_07015"
     CDS             14087..14641
                     /gene="folK"
                     /locus_tag="PKM72_07015"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNP60574.1"
                     /translation="MAQEIVYIGFGSNVGDRQELCDRAVALMNLLPRSCVTGVSSYYE
                     SEPIDPQGILGPTWFYNGVVRLETGLSPQRLLGILQETERALGRDGDNRCGPRTMDFD
                     IIFFGQHVIEQPGLIIPHPRLHQRRFVLEPLVELDPDWNHPSFRRSVKELLSSLEDTS
                     RVNKLDVIPGAKYGSRPACSGPTA"
     gene            14645..15493
                     /gene="panC"
                     /locus_tag="PKM72_07020"
     CDS             14645..15493
                     /gene="panC"
                     /locus_tag="PKM72_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNP60575.1"
                     /translation="MRVIHSVRNLHSWRTKQELLEGPIGFVPTMGALHDGHLSLMQRA
                     RKQCRTVVVSVFVNPLQFGETEDLGRYPRSFRDDRRLCQEAGVDLLFAPPPEEFYPEN
                     FQTTVRVNSLTQRWEGEARPTHFQGVTTVVTKLFCSVRPHRAYFGQKDYQQSLVVNQL
                     IDDLNWDMRMIRCPTVREPDGLAVSSRNQYLSAHQRVEAGLLSKALRQGSDAIRTGVR
                     NVRTIQARMEKILGNNSEVHMEYLAICDSKNLEPLTRLRGTVVILGAVRLGTIRLIDN
                     LLVRCP"
BASE COUNT         3739 a         3690 c         4220 g         4031 t
ORIGIN      
        1 ataagccggc cgccgcttgt ttgtcttcac gaattacccg tgggctcccc attacgaagg
       61 cctatctttc ccgagtagaa caggccgagg cctttttggt ggctcaaggg attacgcaag
      121 tgcgggttcg ccttcacggc gatttggcga gaatcgagat agacccgacg caaatgggaa
      181 ttctgatgga agaaccagga agatcccggt tagtggagaa atttaaaaag cttggatttc
      241 aaacgatcac acttgacttg gaagggtatc gggccggagg agggaacctt ccctcaggtc
      301 cggtttgaag cctataccaa aattgttgcg aatgggttaa ccgggagagt tttacttatg
      361 tactattgtc tgatgtgttc ggggaggcaa aaaaagggaa gcgatgcttc cctctttttt
      421 ataatgcacg aaggtttgat aggagcgatc ccggaaagaa tgcctcctgg gatggaggat
      481 cttctaaaaa aattgggaaa tacttagttt tttgattggg cggcgactgc ctctagagct
      541 tcctgtgcaa agcgccgatg gtcatcgtcc gataaacttc gaccaattac cttctcggca
      601 accaaaatgg ccaactcagc cgattgggtt ttaatgtctt gaatggcctt ccgccgctca
      661 cgatcaatgt cttgcgtcgt ttcctcttta atcctctgtg attccacgcg aagccgttgc
      721 tcgttttcct caagtagccg ttgagcctgt tgcttggcct ctgtcaggat cgtttccact
      781 tctttggcag ccaactgaag cttggtttca tattccttga gctttccctc tgctgcaact
      841 cggttctgct cggcctggtc gaggctttct ctgatctttc gttcccgggc ttccagcgtt
      901 tccaggatcg ggggcaaagc gtacttatag agcacccata gaaggatccc aaatgagagg
      961 agttcccaga atatcagtga cgaaaagaaa tgtgagtcaa attgtggcat agatcaggag
     1021 ccctttatcc tttgcccatg atgataaagg cgatgaccag cccatagagc gcgatggctt
     1081 cgaccagggc gaacccgatc cacatatact tgccgaccct ggcttctgcc tctggttgac
     1141 gggcgactgc ctcaatcatt tttccaaaaa tatacccgat gccgatcccg gcgcccgcaa
     1201 agcccgcggc cgctaggccc attcccaaaa gtgctgctgc tgcagaatcc attgtagtgt
     1261 tgtcctccca gtttaaaagg gactaattgt gagtgttccc ttatcgtttt gttcgagcgt
     1321 gatttagtgc agatgcatgg cgtcaccaat gtagacgcag gttaaaactg taaaaatata
     1381 ggcttgaata aaggcaattc cgacttccaa gccatagagt gcgatggtaa atgaaaaagg
     1441 cagccaccca atcataagcc cgcctgctat ggtaaggccg aacaaaaccc caagaatgac
     1501 atgtccggca gtcatattgg caaataatct gacggccaga gagatcggtc tagccagttg
     1561 gctgattaat tcaatgggaa tcattagggg aaggagccat cctggtgtgc caggaggaac
     1621 aagtatccca aagaatttag cgccatggag ttggaatcct atgataagac tgaggagata
     1681 gacgcctaca gcaaacaccc cggtcacaat aatttggctg gtaatcgtat aagatccagg
     1741 tatgagaccg accaggtttg aaaataagat aaacaaaaag agggcactga tgagggggaa
     1801 atatcgcatc ccagaggccc ccatcgtgtc ctgaataatt ccgcgaataa agtcgaccag
     1861 caactctcca agactttgga gcttagaagg caccaatgcc ctggcgccac cagctttcat
     1921 caatagaagc gtaacaagcc ccaccacaac ccacatcatc aagacggcct tattgacgga
     1981 aatgtcaagg ccgaaaaagg aaagcgggag aaagttatga agctcgaagg gatgtaacgg
     2041 atcttccaaa actaaacctt gtgtctagtg actcattgtt tccaacgctg agctgcacgg
     2101 taggcattgc gaactccagc gattaatccc agtaccagtc ctaacgccac accccacggt
     2161 gtggtatcca acacatacga atcaattaac caaccaagga atccaccgac gataagggcg
     2221 gcaagaaggt ccgtccccac tcgcattgcc tgtcccagcc ccgcaaacat tggatcctga
     2281 gaaggtgcca tttttttaat cggccaggcg agtcgtcgga aggggacaca ttcaagcaaa
     2341 aaatcacgaa tcatgtcaag caagaaaggt cccatttttc aataatggta tggtaaggta
     2401 cgcctgatca agacagagaa tcatgaagcc tcttagctgg ttcagtactg ttactaatat
     2461 ttaggccgac attatgatga agaagcagtt ggtgttcctc ctgatcatgg cgtttgcagc
     2521 atatgagaat cccctgggag ccacttcctc aggggtggat gatttgccgg atttaacggg
     2581 tggggtggtg cctgttgttc aatataagca gctcgatccc ttctccggta acgatatatt
     2641 aacgtctgtc agtgaagtag aatatacggt gaaggtcaaa aaccaaacgg gggatccgtt
     2701 gattgcagat tccttaatcc tggtagtgga ttctgtgctg gaaatttccg ggaaagaaat
     2761 ttccaatcga atcaaaatcg agggatcgga tggtatgact ggggagggaa aacatttttt
     2821 caggattccc agtaatcgaa aggatcttcc accctatggg gaaagtgaaa cggtcaccat
     2881 acgggtgacg aatcctgatt ttcttcggtt ttatcccccc ggtgtacggg ttcgcgggat
     2941 ccgacgctct tctgaaaaag ccgccaaaga tctgcttgat aacctggtgc aagaggggtt
     3001 gttgtctccc gaggacgcca tgcgggcctt agaaaatcct tcatcagata gtccttaacc
     3061 cttgtcctcc attcccaagc atgccgaacg aggcactcat gcctcgcttc attcgacctc
     3121 gtgttttccc cttattcagc actgctcccc cgcttttgaa gatccctttg atctctttgg
     3181 ccgggtgact ggttacgctc cacattcatt tttcatggag catgacgccg ttcaagatgg
     3241 ggtgtcctgt cgtttttctt atttggggtg cgatccctat cgcgtggtcc gggggaaggg
     3301 gcacgcgtat gaatcggttt cgttgggtaa aaaggaaaag cacatcggtg atccattcgc
     3361 gatgttgcag cgtaccttcg cgaggcagac tgccggttcg gggcaccaat tctcgccgtt
     3421 ccagggagga gcgatcggct gtttcagcta cgaccttgcc cgtacgtttg aggttctgcc
     3481 cgagtgtctg ccggatgatc tgcattttcc tgacctgtac tttctgtttg tcgagatctt
     3541 tgtggccgtg gatcatcagg ctccgggggt ctggctgata tttgcgcctt cgcctgagcg
     3601 attggcgggc gaaagctggg atcacttgtt gcgggaaggg caggcgcgcc tgtctgatct
     3661 gcaagccaaa gtgatgattc ctgagaacct tcaaacgaag atgcattccg ctatgtcctc
     3721 gcttcgcata gagggggagc aatcgtcttt ggaatatatg gatcgcgtgc gtgcctgcaa
     3781 gcacttcatt gctgccggag atatttatca ggctaatctc tctcatcgat tccgggttga
     3841 gggagtgact catggttttg caagccaagt cgaagctgga gctgacatat atcggcaatt
     3901 gcggaaggtc aatccttccc cccattcggc cttcctcgtg ttggagtctg atgtcatcgt
     3961 gtgtaattct ccggaacgat tagtacggct ttcaggggga cgtacggata tgcgtcctat
     4021 cgcgggtacc aggtctcggg acatagagcc tcaagttgat cgacggttgg cggaagatct
     4081 gttgtcttgc cccaaggaac gggcggagca tctcatgttg gttgatttgg ctcgaaatga
     4141 cctggggcgg gtgtgcgact acggatccgt tcgagtcaat gagttgatga ccgtggaacg
     4201 atattctcat gtcatgcata tggtctccca ggtttccggc cgtttgagtg acgtctgcca
     4261 tggtttcgac cttattcgag ccacgtttcc tggagggacg attaccggag tgcctaaagt
     4321 ccattgcatg gaactcatcg aacagcttga gcccgttcgt cggggaattt acaccgggtc
     4381 gattgggttt atcgggtgga acggagatct tgacttgaat attgccattc gcacgttatg
     4441 gctcacggcg ggacagggat atctccaggt gggagcgggg attgtggctg attcggaccc
     4501 tgagcgggag tataaggaaa cgctcaaaaa ggccgaagct tttttccagg ttctgaaagg
     4561 gaattactga cgacgaaacg ggtataagag atgtggatct ttctcaacga tcagtttgtt
     4621 aaaaaagagg atgctcgaat atcggtcttt gaccatgggt ttttatatgg ggatggtgtc
     4681 tatgaaaccc tccgtgtcta tcaggggcgc atttttttat tagaacgaca tttggcacgt
     4741 cttcgacgat cttgtgatct gatcggtctg gttcttccga tccaggatga cgcttgggaa
     4801 gccatcatga ccgaaatgtt gattcgaaat gaacttgagg atgccagtct ccgcataacg
     4861 atatctcgag gtgaagggga actcgggatt gaccccggtc tttgtccccg cccgactatt
     4921 gtcgtgatgg cgaggtctgt ggtggcctat cctgcccaaa tgcgggagca gggagtcagg
     4981 gtgcaactcg tttcagttcg acgaaatccg gaatctgccc aatcacctca aattaagtcg
     5041 ctgagttttc tcaacaatat tctggccaag caggaggccg tacaggcaag agcgtttgac
     5101 gccctgatgt tgaatatgga cggtcatgtg acggaatgta cgacgagcaa tatttttttt
     5161 gtggcaaacc accgattaca tactccttct gttgcctgcg gaatcctgga ggggatcact
     5221 cgagaagtgg tgatcgcctt agccagggat ttaaaaattg acgtggagga aggggcgtat
     5281 tcgcctatgg acatgcttca agccgatgaa tgctttatga cgaacacagg cctggaagtg
     5341 atggcggtct ctcaaattgg agaagatact attggtcaag gaaagagtgg gccaatcacg
     5401 atggtattat ggaagggttt ccaggataat ttggaaagat ggttggggcc ggttatcact
     5461 cgccctcaat agacataaca ttcaacgcgg tgtggtattt tttgtattct ttggggtaat
     5521 aaccgcagtg ggtttggcgc cattgtcctg ttgacaccgg ctatgagttt tccaggcgag
     5581 tcctttatgg aacggaaaat cttcacggaa atgggctccc acgctattgg atcgccaaag
     5641 tgcagcttcc gcaatgcatt gtccaaccaa aaccatattc cgggtttcta gtccaggccg
     5701 gctccacggg gctgctgaaa gcttggatga ccatagctga gcctgatcga cggttttctt
     5761 tagagaattc cccgttcgca ccaatccgac tttagaccac attagacgcc gtaaggagtt
     5821 tctgattttt tccacatctt gagtggacat cttccgtccg ctgggttttc ggaggagcgg
     5881 agaatttgaa aagtcagggc aggagctgat cgtcgaacaa gaggaagcgg cttgggctgc
     5941 ccgatagcca aagaccagac cttccagcaa agaattactc gcgagtcgat tggcaccgtg
     6001 aactccggaa caggctactt cccctgccgc aaacaggcct gggatggtgg aagccccatc
     6061 caggtcggtt ttaacgccgc ccataaaata atgagcactg ggggaaaccg ggatccattc
     6121 ctctgtgatg tcaatatcgt atcgtaagca ggtcgagtag atggtaggaa accgttcttt
     6181 caaaaatgcg gcccccaaat gcgtcacatc taaatagaca tgacgagctt tggttcgttg
     6241 catttccgtc cagatagccc gggagacgat gtcccttggt gcgaggtcct gacttcgatg
     6301 atagtttttc atgaagcgct cgcagcgatt attgcgtaaa atccctcctt ctcctcgtag
     6361 agtttcggac agcagaaaag gaggactgga tgggagatac agggcggttg gatgaaattg
     6421 gacgaattcc atatcctcca gaatggcacc cgcccgataa gccatggcaa tgccatctcc
     6481 ggtcgcattg gccgggttgg tggttcgagc atagacctgc ccggcccccc ctgtgactaa
     6541 aagtgtggcg ggagcacaga cgatcttaag agtcccactc aattcatcaa ggattaacgc
     6601 tccccagcat cgtccgtcct ggatgcatag ctcaaccgca acatgatttc ccatccaggt
     6661 caggtttttc agggtttggg ctttgacgct gagtgcacga accatttcgc tgccggtggc
     6721 atcccctccg gctcgtaata ctcgatgacg gctgtgggcg ccttccctgg tgaacgcgag
     6781 tttgccatcc actgtgtcaa atttcgctcc ccattcaatc aattcatgaa tacgggaggg
     6841 tccttcttca acgagtattt tggtggctgg ccgggaacag agttgatggc cggctttcag
     6901 agtatctgcc aaatggagaa ccacgtcatc ttcctcgctg agcgcgaccg ctacgcctcc
     6961 ttgggcataa aaagaattat tgtcttgtgg cccacctttg gcgaccatgg tgacacgacc
     7021 atatcgactt aattcaattg cagctcgaag gcctgccacc cccgcgccta aaatcaggaa
     7081 atccgtcgaa atcacgtggg aggaatgatg tgatgcggca gcggaagaca tggaaatgat
     7141 tggagcagtg aaaaagttga tggttaagaa gaaaaattga gacggtgtgt catggcaaaa
     7201 ggcaggtttc cttcaatccc ccggagcagg atgacttgat tgccactcca cagcaggacc
     7261 ccatgaccgc tggcttgagc gggcgcttcc tgaggggagc gttcccagtt gccttgccag
     7321 gagacaaagg ccgaaatacg atctccatca atcaccatgc gatcgatggt caggtccaga
     7381 tgaatagtgg taaacgtagc gaagtcttct cgaaccttgg cttctaagag gtcaaggttt
     7441 tcaaggggaa ggagtaggtc ctggaagccc gatgcatcgt gattgacata ggctttttcc
     7501 agtgttttga tagcttgcac tactcgttgg aatcgggcgt ggtcctcagg gtatttgggg
     7561 gcttttttcg aacatcctga tcctgcgagg gaaataagga tcggaaacgt tattaaaagc
     7621 aacaaaagat ggttatttaa aaatcgaagc agaatcatga aattctataa tgaagaagaa
     7681 tactggaggg ggtcaagaga atttatagaa agtagatgat taatgagtga aggaacaggt
     7741 atgatgtgga tggtttcctt gacacctttc ctgaccctat gataggtagt cagccggcag
     7801 tcggggactt gtttgccaaa ttcagcgcga gactctctga accaagatag ccgcatcgaa
     7861 ttcacccagg aatgtacctt tatgtccagt gttgtcaaaa agcgacggaa gaaaatgcgc
     7921 aagcataaac ataaaaaact ccttcgccga atgaagtttg ccaaacgtcg taattaattt
     7981 ccataggcgg gatggtcaag ggcttgaata tgacccaggg gaaaaaaact cgtattcggg
     8041 tccgttcctc tttgtcgtca tatgtcggag atctcttaat cccgcctatg cgaaatagaa
     8101 tttcagatgt gttaaatgat gagtctgtcc tgtttattaa cttaacggat gtgctgatca
     8161 atgacactga acattctgcg tttgtcgctt taaacaaaaa tcaaatcgaa tcggttattc
     8221 aactcgacta gcctccattt tgtcatttct ctcgccttgt cgcaccctcc gattgtgaat
     8281 tggtgacgtc gcttcttacc tctaaacgga tttaagcacc tgttcgtgta aggccattat
     8341 gaactatctg tctcgattta ttcccttcct ccgtccctat ctgatgacca tggtcggtgc
     8401 gggggttttg gtgatgggtg tcgcggcctg caacctgttg ctcatcaggt tgggcggctc
     8461 gctgtgggat cttataacgg ttcaacggga cttgccgaaa ttgaccagca tggtgtggat
     8521 ctttgtcggg ttggtgatcg gccagggcct gttatccatg gctcatagtt atttgaccgc
     8581 gctggcttcc cagcatgtaa tggcggattt tcgtacccac gtcttctctc atctccatcg
     8641 gttatcactg aatttctttg cgaaacggcg aactggagag ttgatttccc gattgatgaa
     8701 cgatgtcggg gtgattcaga atttattaac ggagaccccg atggatgccc tgaaacatct
     8761 agtgaccatc attgggggcg tgggatttct tttggtgatg aattggcggc tgtgtctctt
     8821 gattctcatt ctcttgcctc tgctggccct tgtcgccagg atgtttggga agcggctaaa
     8881 agtcttatcg atgcaaattc aggatcaaac cgcccatgtc accacgttaa tagaagaagt
     8941 ggtgtcaggc attagggtgg tcaaatcgtt tgtccagggt aaacgggagg aaacacggtt
     9001 tcgttcagct gtagagacct tgttagcgac caccatgaaa cggaccgcgg tgttggccgt
     9061 gtttgttccc gtcatcacct tctgtacgtt tgtgatggcg attggcgtct tatggtatgg
     9121 gggcaaacaa gtcatcgaag gccagctatc acctggggaa ttatttgcgt ttgttctatt
     9181 tgcggggatt ttaattggcc catttgggtc agcagcccga atgttctctc aggtgaagga
     9241 agtgcaagga gcgatgacgc gggtgtttga gctcctggat actccactgg aaattcagga
     9301 tagtccgatg gccaaggcct taacgcccat tcacgggcag gtgcagtttg atcacgtgca
     9361 atttagctat gagggccgtc cccctgtttt ggatgccgta tctttctcga ttcaggccgg
     9421 ggaatgtgtg gccctggtgg gaccaaccgg agccgggaaa acgactattg tgaatttact
     9481 gcatcgtttt tatgacccaa ccgccggccg ggtgctggtt gacgggcatg atctcacctc
     9541 cattcaagtg gatagtttgt atcaacagtt ggcgcttgtg cctcaggaaa cactgctttt
     9601 tggtggaacg atcgcagaca atatccggta tggtcgagcg gaagcttctg aatccgacat
     9661 gatagaggcc agtcgccgag ccaatgcgca tgaatttatt caggcgcttc ccgagggcta
     9721 tgataccatt ctaggtgaaa aaggaattaa tctgtctgga ggacagcgac agcgcatcgc
     9781 tattgcaagg gcgctactca aagacccgag aattttgatc ctggatgaag ccacctcagc
     9841 attggatagt cagtcggaat cgttggtgca gtcagcctta acggaactga tgaagggccg
     9901 caccacgctg atgatcgcgc atcgcttttc ctccattcag cgggcagatc gaattctcgt
     9961 cttacataaa ggcacaattg tggaggaagg acagcacgaa gaacttcttg ccaagcgggg
    10021 actctaccat catctctata cactgagaga ggttgaggga ataacggagc gtatggagat
    10081 cgcagaacaa cccttgcctc aataaagggt catagagccg gaaggaaaac aaacaaaacc
    10141 ggttctgagt cctgatccga ttgagacgag aatagttctc aatcctctgg catttttatg
    10201 cctgggtctc ataaaaatcg ggttaatgaa ttttggagaa gtcggctggt atgttaatca
    10261 gttttccatc gggaaagaac acggcgaggg ttcctggagc tagtggatcg aagtcggcat
    10321 aggcaaagcg tgcggtaaac ctcgaccgaa agctgacggt atctcctgga ttttttcggc
    10381 cgcgctcagg agggccaata tccacggggc gaatcgtgtt tgagccttgc tgaagagcaa
    10441 tatctgcccc ttccgcaaaa aactcatctt ccccgcataa gcgaatttca acttcaaggt
    10501 agggcatttc cagaatttcc tgaattttag cctccggcat acgaatgtct tgtttctggc
    10561 gtttggattc cgccgcttcg cgtcggccaa agtattccaa ccaatagagt cgtgttctta
    10621 atatggcatg tgccccacag ggttccactt ccgggttagc gccgactcga actgcacgtt
    10681 ccgaggcttt catcactgta gccacatcct ctactgaatc ggcttgttcc atggttttgc
    10741 gaccagatgc catcgcctca ttggcttgct ctaatgtgag agacaggtcg atcccggagg
    10801 aaatggatgg gtgagtccct acaccgatta tagtgagggc acaaagagta agcgggagac
    10861 gaatgtacga agacagcaat gaaaccatgc gcattctcct tggcactttt cgggcatgac
    10921 tcaggatgaa agtcggaatt gtagaggaca cctgaaatct ttgcaatccc tgccctatgt
    10981 acggcttgag agggatcatg tccgtacata tacacagttg gggagtagga gtaactggaa
    11041 aagtgatttg tagttgactc cctcatgccg ctccggtaga ttagcggcct gtttggttga
    11101 aattaggtgg accgtccccc tacggcacca gttatttggg gttctcgcct caggtaagca
    11161 atcccttatt acacgaaata tcatgttgaa cgtcaatttc ccttgaaggg gtgtggattg
    11221 cgaaggacat catcatttgg agaaacaatg aatattgaga aagttgagcg tgtatacagt
    11281 gcgtacgcgt ctgtttatga ccatacgttt ggtaaagtct ttcagcaatc tcgcgagtcg
    11341 gcggtgcgcg gccttcctat tgagcccggc catcgagttc tcgaggtcgg cgtaggaacc
    11401 ggagaggcgc tccctcttta tccctcctgt tgcgaagtcc ttggtattga cctctcggat
    11461 ggcatgttgg aacatgcccg acgtcgggtc caggagcacc ggttggatca tgtgaccctc
    11521 aagagaatgg atgcggggaa aatggatctt cccgataatc actatgatat tgtgatggct
    11581 gcttacgtgg tcacagcagt tcctgattat cgaagcgtgg tggatgaaat gatccgggtg
    11641 tgtaaacccg gtggccggat catcatgctg aatcacttta gcaatgggaa taaagttatt
    11701 gcggcggtgg aaaaatggat ttctcctttc tgtaagcata ttggattcag aaccgatctg
    11761 gcattgcaca cggtcctgga aggcacgtgt ctgatggtgg tcaataaaaa gaaagtgaac
    11821 cccttgcggt tctggcattt ggtggaatgc gtgaataaaa agaatggtca acaggctttg
    11881 ttcacaaatg gttttgcatt gaatggacat tcatagccct ctccacctat tgtcgtgccc
    11941 tgtatcccct tgcctccgga cattttgccc tattctcagg tagaccacgt cacgaagggt
    12001 aggactagtc cttcgtgaat cggcttcttg ccctgaacca aacccaaacg cggcgtcatg
    12061 aaagttgccc tctgcctcca acatgtcttg tttgaaggcc ctggcgtttt tcgtcgggct
    12121 ctagaaactc gtgggtatgc ggtcagaaaa gttgtcgtgc ccgctgaggg tttgcctgca
    12181 gacccatggg actttctcct cattatgggg ggaccgatgt ccgttaatga tggtgatgcg
    12241 tgggtcgagg ctgaactcca atttgtgaag acggctctcg cgaaagatat tcctgtcttg
    12301 gggatttgtt ttggagctca gttactggcg aaagcattgg gtggttcggt gacaccgggg
    12361 ccgaagtttg aactcggcat gagtgccgtg tccctaaccg acctgggaaa agtcgatcct
    12421 atcctgagtg ccatgcccca ggactttctc gtgtttcaat ggcatggaga agggatcacg
    12481 cttccgcctg gaagcgtgca tctggtgact tctgctgatt ttccagttca agtattccgg
    12541 atggcggatc gaacatatgg cctcctgttt catctggaat tagaggaatt gggcatagag
    12601 gccttgtgcc gggaatgtcc tcaagatgtt cagcgaggtg ggatgacacc ggagtcaatt
    12661 caagccccgt cgcgacccca cctacccagg ctccatcaat tggctgatcg cttcattgaa
    12721 catttgattc aggcatgatg acacctcctt cttgattcat tttcccattt cccgtttcga
    12781 aagcattttt tcctgggcgg taccggaacg ggttacatat ttggtaccgt agcaggttca
    12841 tattcaccgg tttttgataa ggagtacgaa ttcatggctg agtttcctat tcaatatgca
    12901 gagcggatcc gcaccttacc cccctattta ttcgctgcca tcgatgacat gaagcgtaag
    12961 gccatcgagc gggggatgga tatcatcaat ttgggtattg gcgatcctga tttgcccaca
    13021 ccagaaccga tcattgaatc gttacgacgt gcggcgggaa atcccaaaca tcatcagtac
    13081 ccgtcgtatg atggcatgtt gtcatttcgg actgcggtgg ccgattggta tcaacgtcgg
    13141 tttggcgtga ccctggaccc caaaacagaa gtggtgacat tgatcgggtc aaaagaaggc
    13201 atcggccatg ttccgttggc ctttgtcgac ccgggagata ttgtgttggt tccgagcccg
    13261 ggatatccgg tatacccggt cgcaaccagc tttgccgggg ggatttccta caccatgcct
    13321 ttgacaaaag agaatgggtt tttgccggat ctatctgcca ttcccaagga tattgcccaa
    13381 aaagccaaaa tgatgtttct caactccccg aataatccta cgtcggtggt ggcgagcaag
    13441 gaatttttca aaaaggtggt ggactttgcg cgtgagcatc agatcattgt ctgtcatgac
    13501 gccgcctatt ccgaaattta ttatgacggc aatcggcctg cgagctttat ggaggcagcg
    13561 ggtgcaaaag acgtgggggt ggaattccac tcgctgtcga agacgtacaa tatgaccgga
    13621 tggcgcatcg gattcgccgt tgggcgaaaa gaagtcatcg cgggtttggg gaaggtcaaa
    13681 accaacattg attcaggcgt gtttcaagcg atccaggagg caggcattac cgcgttgcaa
    13741 ctggatggtt cagtgacaga aggcttgcga acgatttatc aagaacggcg ggatgttctg
    13801 gtgcccggct tgaaagccat gggattggaa ctggattcgc ctccggcggc gttttacgtt
    13861 tggatcggtg tgccgaaggg attcacctcg gcgtctttca cggcgcatct cattgagaaa
    13921 gccggaattg tgacgacgcc cggcaatgga tttggcgatc cgggagaagg gtatatccgg
    13981 atgacggtca cgaccacaaa agataaattg gccgaagcgg tggaacgcat gaaaaagatt
    14041 ggctggtagg tccttattca tttcggctaa tctggtaaac atcgaaatgg ctcaagaaat
    14101 tgtgtatatc ggttttggtt ccaacgtcgg agaccgtcag gagttatgcg atcgggctgt
    14161 ggcgttgatg aacctgctcc ctcgttcttg tgtcacaggc gtgtcttcgt attatgaatc
    14221 tgaaccgatc gatcctcagg gaatcctggg acctacctgg ttttataatg gcgtggtgcg
    14281 gttggaaact ggtctcagtc cccaacgact tctgggcatc ctccaagaaa ccgaacgagc
    14341 gttgggccgt gatggagata accgttgcgg cccccgcaca atggattttg acattatctt
    14401 ttttggccaa catgtgatcg aacaaccagg actcattatc cctcatccgc gtctgcatca
    14461 gcgacgattt gtcttggaac cgcttgtgga attagatcct gattggaatc atccctcctt
    14521 tcgccgttcg gtgaaagagt tgttgtcgtc gcttgaagat accagtaggg tcaacaagct
    14581 ggatgttatc cccggggcga agtacggctc ccgaccggcc tgctcggggc ctacagcctg
    14641 atccatgcgc gtcatccatt ctgttcgaaa tctgcatagc tggagaacca agcaggaatt
    14701 gttggaaggt ccgattgggt ttgtcccgac gatgggcgcc ttgcatgatg gacacctgtc
    14761 gttaatgcaa cgtgcgcgaa agcaatgtag gaccgtcgtc gtcagtgtct ttgtgaatcc
    14821 gctccagttt ggtgaaactg aagatttggg ccgctatccg cggagttttc gcgatgaccg
    14881 gcgcctgtgt caggaggcgg gtgtcgactt actctttgct cctcctccgg aagaattcta
    14941 tccggaaaat tttcaaacaa ccgtcagggt gaatagcctc acgcaacgct gggagggaga
    15001 agcacgaccc acccactttc aaggtgtcac gactgtggtg acgaaattat tctgttcggt
    15061 tcgtccccac cgcgcctatt tcggacaaaa ggattatcaa caatccctgg tggtgaacca
    15121 gctcatagat gatctcaact gggatatgcg aatgatccgt tgtccaacgg tgcgggagcc
    15181 cgatggccta gccgtcagtt cccgcaatca gtatctttca gcccatcaac gcgtggaagc
    15241 cgggctttta tcgaaagctt tgcgccaagg gagcgatgcc attaggaccg gagtccggaa
    15301 tgttcgaacg atacaagcca ggatggaaaa gatcttaggc aacaactccg aggtgcatat
    15361 ggagtacctg gcaatttgtg attccaagaa tttggagccg ctaactcggc tgcgtggaac
    15421 ggttgtcatc ttaggagccg tcagactggg gaccatccga ttgattgata acctgctcgt
    15481 ccgctgtccc tgagttcagg gttacctgtt aataaggttg ttcggaagtt cgttgggatt
    15541 gggaccagag ggaactaccg gaaaatattc ggctaagacc tcaccacagt gggtaatggc
    15601 ctgacacaac gactccacgg gatttccttt gtggaagcct tcttgaatca ggctcagcat
    15661 tgtgttccag gtctccgcgg
//
LOCUS       DAORAQ010000056        15637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_641_length_15637_cov_68.234630, whole genome shotgun sequence.
ACCESSION   DAORAQ010000056 DAORAQ010000000
VERSION     DAORAQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15637
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_15637_cov_68.234630"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..836
                     /gene="bcp"
                     /locus_tag="PKM72_07025"
     CDS             153..836
                     /gene="bcp"
                     /locus_tag="PKM72_07025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNP60576.1"
                     /translation="MASKAKKAQPKKKGPLPSKPTKPKVAAKKKATPKPSSSTGQGVA
                     STSRKTSTTVKKASAPKTKKPAQVSPRIVSSRELQVGDPAPAFSLPDDTGNIVNSSEL
                     RGKKVVVYFYPKDDTPGCTTEACSFRDGIQELRKSGAVVFGVSADSVSSHRKFSDKFQ
                     LNFPLLSDESKAMIQAYGVWKEKSMYGRKYMGIERTTVVIDEDGTIRNIFPKVKVNGH
                     FSEVLESLR"
     gene            complement(875..1318)
                     /locus_tag="PKM72_07030"
     CDS             complement(875..1318)
                     /locus_tag="PKM72_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNP60577.1"
                     /translation="MSSSTFHLSIPVKDIESTKEFYAQGLGCGVGRQSSVAITLNFHG
                     HQIVAHVTEDLGPQQKSIYPRHFGLICRTEEEWNELRDRAQGNRLIFFREPRRRFPGT
                     PLEHLTFFLEDPSHNLLEFKYYLNANAIFGEEAFSQIGDAHEQNG"
     gene            complement(1438..2358)
                     /locus_tag="PKM72_07035"
     CDS             complement(1438..2358)
                     /locus_tag="PKM72_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HNP60578.1"
                     /translation="MPKSTPIPTPRVLIHGGAGSRQPTPSQSMCLTEALIGGYECLKT
                     GHSPLDAVESMICYLEKSGLFNAGRGSLRQLDGFQRMDASIMEGQSLSAGAVAGLEGY
                     LHPICAARRIMADTDHVLIVGRHANRLARHFGLARLVHRKSSTPLMNKRNSRNTLNPK
                     SQALYRQLGLYDTIGAVAMDRAGHLAAGASTGGVAVMLPGRVGDTPLIGAGVYADDTA
                     GAISMTGLGESIIRAGMAKHMAILLGLGWTPLKAANYALKALVSRIQGEAGCLILDRT
                     GRFAIRHTTPWMSAGYWNGRGKPVVKSRFP"
     gene            complement(2412..3929)
                     /locus_tag="PKM72_07040"
     CDS             complement(2412..3929)
                     /locus_tag="PKM72_07040"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNP60579.1"
                     /translation="MTPELKKKLQSCPSLPSPTHVAVQIINLANEPETDLHSIIRILN
                     CDPALASKILRIANSPLYPYCKKVESLHQAVLVLGLNATISLALSFSLMKSLQTSRGT
                     GLDYGLYWKRALLAGTASRALGAACGLVEIEELYLSSLMQDLGMLALDQVFPDLYNPP
                     NLDQTSHARIISHEYQCLGVNHAAVGSWLLTQWNFPDRLRLAVAGSDDPSRIPQGDEL
                     AKFVHCVSLSGKIAEIFLRDSQSAYIQEVRENAQTSLNLNPAELMEVLETTQGLLPET
                     EKIFRTDLQPWTEPQAILEKAREALLLRSLQTLKEVQELQQNNAAMEAKFHNLEEIHR
                     HDPLTGALARAYLDKYLKASFEHALVNQECLTLVFGDLDKFKSVNDTYGHQAGDGVLQ
                     STAHLLQSKLRTTDSVGRYGGEEFVLILPKASSATAVMVCERILSAFRGTTHDIAGDK
                     LIVTISLGIATHTPDHPYPTVTDLLHAADEAVYYSKTQGGNRYTSYDKIQTEQSV"
     gene            complement(4370..4933)
                     /locus_tag="PKM72_07045"
     CDS             complement(4370..4933)
                     /locus_tag="PKM72_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60580.1"
                     /translation="MTPIPEQHPLRQLFGRLTERNFSEALGWPDFQVTEYVSNLLLEF
                     IHIDQLARIKDHEGEPVETVVELLYAAESHSQQSSAEKERDIHRHIGDLTLFLAGLFP
                     EYCKRIKCSGLIHHKDFLVDYVKAGKRSYSIVATHQDPQGKNPPALFQTLSTNFELCV
                     AGLGFVRLDLDRFQDPSLNQAQSHLFH"
     gene            complement(4989..5486)
                     /gene="dtd"
                     /locus_tag="PKM72_07050"
     CDS             complement(4989..5486)
                     /gene="dtd"
                     /locus_tag="PKM72_07050"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNP60581.1"
                     /translation="MIAVIQRVRQASVTVENQVIAHINRGLVILLGVAQGDTGQDVTF
                     MVEKIPRLRIFPDTQGKMTDALQDINGELLLVSQFTLLANTEKGRRPSFELAAPPEEA
                     RMRYQETLEQFLALGLSVQAGKFGATMVVALDNDGPMTLILDSRVGKGVKKVKNLLHQ
                     ADRHT"
     gene            complement(5561..7180)
                     /gene="murJ"
                     /locus_tag="PKM72_07055"
     CDS             complement(5561..7180)
                     /gene="murJ"
                     /locus_tag="PKM72_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNP60582.1"
                     /translation="MSQSAQPPSVPSSPQGETSRISRAAGLIGVATFCSRILGFIRDV
                     ILANLFGANASADAFYIAYRIPNLLRELFAEGSMSSAFIPIFTEYHSTRSKQETWELA
                     SAAFTTLLTLVTFVTLMGIVAAPSLVWVLAPGIHGQANQLATTTLLTQIMFPYLLFVS
                     LAALAMGILNSLHSFAVPALAPVFFNLCVIFFAVAISPLFDQAILAVAIGIVVGGLAQ
                     FLMQLPSLHKQGFPLYWNFHPRHPGVKRMGLLLIPSLLGLAVTQINLTVSTILASYFQ
                     GGPTYLFYGMRLIQFPLGIFGVAMATAILPTLSGQAARGAMHELRETVNFGLRMVLFI
                     ILPSMVGLMMLRIPIIHVFFEHGAFSAIDTVGTASALFGFSIGLWAFASYRILATAFY
                     SLQDTKTPAIVAVVSVILNIVLSLWLMQWLQHTGLALATALAAMGNTLILLTILGNRL
                     QGLLWKTLGLSLVRTGIALVPLIAVSAWIAGFPLWQTTGQSLEKIAWLSLALAASTIG
                     YFSVHHAFKSEELTHLKLMLRRKIRKPSTTT"
     gene            7378..8919
                     /locus_tag="PKM72_07060"
     CDS             7378..8919
                     /locus_tag="PKM72_07060"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNP60583.1"
                     /translation="MTRSSLTQSSLPRWWCFATAFSTGAVVMALEILGSRLLAPVFGS
                     SLFVWGGLIGVVLAAMSSGYAAGGWLADRYAPQRVLAGLLLLSGVWTLLLSWAGQPVV
                     FGVSRWIQDPRWGPCFAASVLLAVPAFGLSGVLPALLRLAIGDIRHLGRHTGGMIAIS
                     TIGSLVGTWGTSFYLLTWLGSTTLVAILGVVQICLGLWWTLRAGILQPGLQVILLSCV
                     GGITWMGFHPVTILPPAIYQEDSPYQQVRVSENDLFRYLILDNTFHAVMWKATPESLA
                     LPYSQVMMGVLPLVENPKRGLILGHGGGSLAKWLGKHWPDLELDVVEMDPSVVQAAER
                     YFGYTPPLNHHVYVEDARTFLRNNPYQYDVVWVDVFARHLIPFHVTTHEFFEELRKHV
                     NPEGAVAVNLASSDAPANVRRAQAVLTTMQQTFPEVVPYGVAGPEWLDTAKGSVNLIF
                     FGGKPVSVMRSSNFLDVLARQMNRDRFPSEGFAFFMSQEPVVLAPGEILTDDFSPLDL
                     LQGEG"
     gene            complement(9348..9671)
                     /locus_tag="PKM72_07065"
     CDS             complement(9348..9671)
                     /locus_tag="PKM72_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60584.1"
                     /translation="MAEEKQKIDLSMYGVAEIIKWCITRNNGRIPGCDTAVFKALQAA
                     LAEKPATGDYYTLDQFWKTRKVFEFTQDEVAVVDRCLYDLPNFDGAQLPQIRYKFWPE
                     AVCQN"
     gene            9870..11123
                     /locus_tag="PKM72_07070"
     CDS             9870..11123
                     /locus_tag="PKM72_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNP60585.1"
                     /translation="MGYPASLLVPSPPTYSGERQEELYFSQSLRARLVIPLGLLGLLS
                     LVGCPTREEYHPPDLIYHYLDIPVGKNPTSIRASDFNGDGFTDLITSNIQGNSLSLLL
                     GKGDGSFQDQKTISACQEPRNVAVDDFNLDHVPDFVVACSGDNHALVFLGQGDGTFLR
                     GAQYLVHRTPVSIATGDFNEDFLPDLAVALRNDKIQILLGMGNGKFRLGSLYEYGDTP
                     TSVATEDLNGDKHLDLAVTNGGPMSHAVSIWLGLGDGTFQAPTDYRTGKRPLGVSFAD
                     FNTDRALDLLVINGEMNTITVFLGNGDGTFQEGRESGGDAGPNFGVAQDFDGDKLPDV
                     AVANIQSGSISLLFGNGDGTFHYPPRDYPTPHAPFALTTLLIATDRGEEPGLAVVNNA
                     SSNVSIFLHHGLNLRKSQLSGTPAS"
     gene            11237..11437
                     /locus_tag="PKM72_07075"
     CDS             11237..11437
                     /locus_tag="PKM72_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60586.1"
                     /translation="MRTFGWWFGMGSLLTLAVLLVQGGMRDLIYGSSHSGTWEGILSM
                     GLTVFGGGVLAGCVAVILNRLR"
     gene            11600..11755
                     /locus_tag="PKM72_07080"
     CDS             11600..11755
                     /locus_tag="PKM72_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60587.1"
                     /translation="MLVERQYTRESRRFNAKCINCGFWFDLADVLRFFKRVLESGYNG
                     QKIRETL"
     assembly_gap    11981..12080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12081..12562)
                     /gene="ruvA"
                     /locus_tag="PKM72_07085"
     CDS             complement(<12081..12562)
                     /gene="ruvA"
                     /locus_tag="PKM72_07085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNP60588.1"
                     /translation="MIASLSGVLSSKTPQDAVISVQGVGYHLFIALSTYFTLPEINSA
                     VQVFVSTHLRNDTIQLFGFSTTEEKQAFSLLTTISGVGPKLALSALSTLSVPDLCKAI
                     ETGDLETLGSIPGVGKKSASRIVLELKDKTNRIMISDSHKPSTAAMEPINFLQEEAAS
                     A"
     gene            complement(12672..13427)
                     /locus_tag="PKM72_07090"
     CDS             complement(12672..13427)
                     /locus_tag="PKM72_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNP60589.1"
                     /translation="MGGHSHWSTIKRHKGAQDAKRGKIFTRVIREISIAARSGGDPDG
                     NPALRQAIAKSKEVNMPADTVKRAIQRGTGELPGMQFEEFMLEGYGPGGTALLLEIST
                     DKRNRTVAEVRNILTKNNGTMAEAGAVAWQFQKKGLLVIENQAVTEEQLFDLALEAGA
                     EDVKQTPTGFEVISEPANFEAVKEALQKAQIETSLSELTFLAQNHIQLEGRDAEQALK
                     LMDLLDENEDVSKVHANFEISDELMEKMAAEST"
     gene            13651..14634
                     /gene="fmt"
                     /locus_tag="PKM72_07095"
     CDS             13651..14634
                     /gene="fmt"
                     /locus_tag="PKM72_07095"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNP60590.1"
                     /translation="MRIVFMGTPAFAVPTLQQLLKTEFSVVGVVCQPDRPSGRGKKVQ
                     VGPVKGLALSQNIPVVQPEKMKDPKLMETLRAWEPDLVVVAAFGRILPKTILELPPKG
                     CLNVHGSLLPKYRGAAPIQWAVIDGEAETGITIMLMDEGMDTGAILQQEVISIGQDET
                     SGELASRMAEIGGALLVRTLRGWVAGTVTPSAQDDSAATLAPILTKEDGLMSWGQSAR
                     CLANRIRGLSPWPGVYTFLEGERWGIWKVQIEDQESGILHTTHDAPQAPGTITAITKQ
                     SIRVQTGQGSLHLLEIQPENKKRMHVSDYLAGHRITLGMTFSMGQQDDCAV"
     gene            14728..15042
                     /locus_tag="PKM72_07100"
     CDS             14728..15042
                     /locus_tag="PKM72_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60591.1"
                     /translation="MTDQSSELLAFIQSQIEEINTIHAQAEKALNAVQGKDHVTKWKR
                     KVVEGLAPYVSQTYLQLITKEWLETTYFVGDVFDELADEVDMCRRHLKKLLKDIQTTG
                     IP"
     gene            15100..>15637
                     /gene="rsmA"
                     /locus_tag="PKM72_07105"
     CDS             15100..>15637
                     /gene="rsmA"
                     /locus_tag="PKM72_07105"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNP60592.1"
                     /translation="MMAPLPPYPRKRLGQHFLIDPNLLRKIIDLADLQPNDYVCEIGP
                     GGGALTRLLCQSARQVLALEVDPRMVDFLKEEFSGYSNLDIREHDALRYPFDQLPEPA
                     VVVANLPYNISTPIMFRLLGARPKIRRMVLTVQLEVAKRLTANPNTKDYGVLSVMAQR
                     LADVRFGFPVSRKCFRPVP"
BASE COUNT         3752 a         3482 c         4157 g         4146 t
ORIGIN      
        1 aaaatagagg aatggaacct gtcaaccatt gtacaatggg ctctcatcac cagggagatg
       61 acgagaatct cctggattga ggaagggtag tttcgtccag cgaaagaatt gacacgccac
      121 gttttactta cttgtcatca gaggaggatt ctatggcatc caaagcgaaa aaagcccagc
      181 ctaagaaaaa gggcccgtta ccatcaaaac cgacaaaacc aaaggttgct gcaaaaaaga
      241 aggccactcc caagccttcc tcatcaaccg gccagggggt ggcttcaaca tctcgaaaaa
      301 cttcaacaac ggtcaaaaag gcatccgccc ctaaaacgaa aaagcccgct caggtgagtc
      361 ctcgtatcgt ttcttcgcgt gagttacagg ttggtgatcc tgctccagct ttctcccttc
      421 ccgatgacac gggaaacatt gtgaactcat ctgaacttcg agggaaaaaa gtggtcgtgt
      481 atttctatcc gaaagatgac acgcccggat gtacgacaga agcctgttcc tttcgggatg
      541 gtattcagga attgagaaag agcggggcag tcgtgttcgg agtgagtgcc gattcagtct
      601 cgtcccaccg gaagttttcc gataaatttc aacttaattt tcccttattg agtgatgagt
      661 ccaaggccat gatccaggcc tatggagtgt ggaaggagaa atcaatgtat ggccgaaaat
      721 atatggggat tgaacgaaca acggtggtga ttgatgaaga cgggaccatt aggaacatat
      781 ttcctaaggt caaagttaac gggcattttt cggaagtgct tgaatccctt agatagtgtt
      841 gcccggagaa ccatgacagg taagggaaaa ccttctaccc attctgctcg tgagcgtcgc
      901 caatttgaga aaacgcctct tccccgaaga tggcattcgc attaaggtaa tatttgaatt
      961 caagcagatt atgcgaagga tcctctagaa aaaacgttaa atgttcgaga ggagtgccag
     1021 gaaaacgtcg acggggttca cggaaaaata tcagtctatt tccctgggcc cggtcccgaa
     1081 gttcattcca ttcctcttct gttcggcaaa ttaacccgaa atggcgcggg tagatacttt
     1141 tttgttgagg tccaaggtct tcggtcacat gggcgacaat ttggtgtccg tgaaaattca
     1201 atgtgatggc gacggaagac tggcggccca cgccgcatcc taagccttgc gcgtaaaact
     1261 ctttcgtgga ttcaatatcc ttgaccggaa tagatagatg aaaggtagaa gaagacatag
     1321 tatagattcc tttacgaatt atcgcatgtt ttgaatgatc aatgaaaatc tgttgcaatt
     1381 tttcgataaa cggttagggt tgagcgtttc aaaaacaaag gagctttaag caacccttca
     1441 gggaaaacgg ctttttacaa caggtttgcc gcggccgttc caatacccgg cgctcatcca
     1501 tggggtggta tgtcgaatcg caaacctgcc agtgcggtcg aggatgaggc atccggcctc
     1561 cccctgtatt cggctgacga gcgccttgag tgcgtagttg gcagctttta agggcgtcca
     1621 gccgagcccg agcaggatgg ccatatgttt ggccataccg gcacgtatga tggattctcc
     1681 taagccggtc atggaaatgg ccccggcggt atcatcggca tacactcccg caccgattaa
     1741 gggggtatcc ccaacccggc caggtaacat gaccgccact ccccctgttg atgcccctgc
     1801 ggcaagatgg ccggcacggt ccatggcgac cgcacctatg gtgtcataca atcctaactg
     1861 gcggtaaaga gcttgggatt taggattcag ggtgtttcgt gaatttcgtt tgttcatcag
     1921 tggagtcgaa ctcttccgat ggaccaatcg ggcaagaccg aaatgccgag ccaatcgatt
     1981 cgcatgccgg cctacgatta atacatgatc ggtatcggcc ataattcgtc gcgcggcgca
     2041 aattggatgg agatatcctt ccaacccggc cacagcacca gccgataagg attgcccttc
     2101 cataatcgat gcgtccatcc gttgaaatcc gtcgagttgt cgaagcgatc ctcgcccggc
     2161 attaaacaat cctgattttt cgagatagca gatcatactt tctacagcat ccaggggtga
     2221 atgccctgtt ttgaggcatt cgtatcctcc tatgagtgcc tctgtgaggc acatggactg
     2281 actgggggtc ggttgccggg aacctgcacc cccatggatc aggaccctcg gtgtgggaat
     2341 aggtgttgac ttaggcaaag tgcgaagtga ttaagaaaaa cgggcgaggt gaatcgtatt
     2401 ttttttcata atcaaacgga ttgttcagtt tgtattttgt cataggaggt gtacctgttg
     2461 ccaccttggg ttttagaata atagacggct tcatctgcgg catggagcaa gtcggtgacc
     2521 gtgggatagg ggtgatccgg cgtgtgcgtc gcgatgccaa gagaaatggt gacgatgagt
     2581 ttgtctccgg ctatgtcatg ggtggtgccc cgaaatgcgg agaggattct ctcgcacacc
     2641 atgacggcgg tggctgagga ggcttttggc aggatgagca cgaactcttc tcctccgtag
     2701 cgtccgaccg aatcggtcgt gcgaagcttc gactgtaaga gatgggcggt agattgaaga
     2761 accccatccc ctgcctgatg accataggta tcattgacgg atttaaattt atcaaggtcc
     2821 ccaaaaacca gggtcagaca ttcttggttg acgagagcat gctcaaaaga ggcttttaag
     2881 tatttatcca agtaggctct tgccaatgcc ccggtgaggg gatcatgacg gtggatttct
     2941 tccaaattgt ggaacttggc ttccatggcg gcattattct gttggagctc ctgaacttcc
     3001 ttaagagtct gaaggcttct gagaagaagt gcctcccggg ctttttccag aatggcttga
     3061 ggttcggtcc atggttgtaa gtcagtccgg aatattttct ctgtttccgg aagaagccct
     3121 tgtgttgttt ccaatacttc catgagctct gctggattga gattcagcga agtctgggca
     3181 ttctctctga cctcttgtat ataggcgctt tgagagtcgc gtaaaaaaat ttccgcaatt
     3241 ttccccgaca gagacacaca atggacaaat tttgcaagtt catctccctg gggaatgcga
     3301 gaggggtcat cgctaccggc cacggccaat cggaggcgat cgggaaaatt ccactgcgtc
     3361 aggagccaac tccccacggc ggcatgattg actcccaggc attggtattc atgggaaatg
     3421 attctggcat gggatgtctg atccaggtta gggggattat agaggtcagg gaacacctga
     3481 tcgagggcga gcattcccag atcttgcatg agactcgaaa gatataactc ttcaatttca
     3541 actaacccac aggccgctcc aagagcccgg ctggctgttc ctgccaaaag cgcacgtttc
     3601 caatacagtc cataatccaa gccagttccg cgactggttt gcagcgattt cattaatgaa
     3661 aaggacaagg caagtgaaat agtcgcgttg agacccagga ccaggactgc ttgatgaagg
     3721 ctttcaactt ttttgcagta tgggtataag ggagaattgg cgattcgtag aattttactg
     3781 gccagcgccg ggtcacaatt gagaattctg ataatgctgt ggaggtcggt ttcaggttcg
     3841 ttggctaagt tgataatttg tacggcgacg tgtgttgggc tgggaagact agggcaggat
     3901 tgtagtttct ttttcagttc gggtgtcata aaagactcag ggtcagaaga ccttcgcgag
     3961 tgcctcgaaa gactcggcat cttgggctgt tctaatcctc atatgcccgg atccacgaac
     4021 acgaggatat atccgtgatg ttttctgatt atggacattt cggcatattg ttttgccatc
     4081 tgaataggca tgacatgata aggcctttct ctccttttga ttcgtaggcg atcgtcctga
     4141 tttcctgaat cgaagcgaga aaacgggttt aagaaagcat aagtgaaaag catgatccct
     4201 cgattatgaa gttgatgttc cgtaacattg atgttgactg gattattttt gttgatcttg
     4261 caaaatctgt ggatgatgag acggccctca tggataatct ggtgaggtag cgaatagagt
     4321 cctccgggtg ggggacggga gctaagggaa ctgtttggag gaagagtgtt tagtggaaaa
     4381 ggtggctttg tgcttggttc aggctaggat cttgaaaacg gtcaaggtcc aatcggacaa
     4441 agcctaggcc cgcgacacaa agttcaaaat ttgttgaaag cgtttgaaag agcgctggtg
     4501 gatttttgcc ctgtggatct tgatgggtag cgacaatgct gtaggagcgt ttgccggctt
     4561 tgacgtaatc caccaaaaag tctttgtggt gaataagtcc ggagcatttt attcgtttgc
     4621 aatattcggg gaagagtccg gcgaggaaca gggtcaggtc cccgatgtga cgatgaatat
     4681 cccgctcttt ttcggcagaa gactgttggc tatgggattc ggccgcatac aggagttcta
     4741 ctactgtttc aacgggttct ccctcatggt ctttgatacg ggcgagctga tcgatatgga
     4801 taaattcgag gagcaggttt gaaacgtatt ccgttacttg aaaatccggc catcccaatg
     4861 cttcggaaaa gtttcgttct gtcaaccggc caaataactg tcttagagga tgttgttcag
     4921 ggattggtgt cataaatgcc cttccctctc tttccaccga atgtcccttc gtcagggtgt
     4981 gtcggtttct atgtatgtct atcggcctga tgtaaaaggt tcttaacctt ttttactcct
     5041 ttgcctaccc tgctgtccag gatgagggtc attggaccat cattgtccaa tgccacgacc
     5101 atcgtcgcac caaatttccc tgcttgtacc gataagccaa gagccaggaa ttgttcgagt
     5161 gtttcctggt atcgcatgcg tgcttcttcc ggaggtgctg ccaattcaaa gcttggacgt
     5221 cgtccctttt cggtattggc cagtaaggtg aattgagaga ctagaaggag ctcaccattg
     5281 atgtcttgaa gcgcatcagt cattttccct tgtgtgtcag gaaaaattcg tagtcgagga
     5341 attttctcga ccatgaaggt cacgtcttga cctgtatcgc cttgggctac ccctaataga
     5401 atgacgagcc cacggttgat gtgggcgatg acttggtttt caaccgtgac tgaagcctgt
     5461 ctgacgcgtt gaatgacggc aatcatgaaa ccctttcgtg tgatatcgcc tatctcaagc
     5521 ccagcggtca taaggacggt aggcgtgtca gtttgaatgc ttatgttgtg gtagatggtt
     5581 ttcgtatttt tcgtcgcaac atgagcttca ggtgcgtaag ttcctccgac ttgaatgcgt
     5641 ggtgaacgga aaaatatcca atcgtgcttg ccgcaagtgc caggctcaac caggcaattt
     5701 tttctaggct ctgtcctgtt gtctgccata gcggaaatcc tgcaatccag gcggaaacgg
     5761 cgatgagagg gacaagggct attcctgtgc gtaccagaga tagtccgagt gtcttccata
     5821 gtaatccctg aagccgattc cctagaatag tgagtaaaat gagtgtgttt cccattgccg
     5881 ctaatgcggt cgccagggct aatccggtgt gttggagcca ttgcataagc cataatgaca
     5941 gcacaatatt gaggataacc gaaaccacag ccacaatggc cggggtcttc gtatcttgaa
     6001 gggaataaaa cgcggttgcc agaattcgat agctggcaaa cgcccacaat ccaattgaaa
     6061 atccaaaaag tgccgaagcg gttcccactg tatctatggc cgaaaaggcg ccatgttcaa
     6121 aaaaaacatg aataatcggt atgcgcaaca tcatcagccc aaccattgat ggaaggatga
     6181 taaatagcac catgcgtaaa ccaaaattca ccgtttcccg aagttcatgc atggccccac
     6241 gggctgcttg gccggatagg gtgggcaaga tggctgtggc cattgccaca ccaaatatcc
     6301 ctaatgggaa ttgaatgagg cgcatcccat aaaacagata cgtggggcct ccttgaaaat
     6361 acgaagctaa aatcgtgctg acggtgagat tgatttgggt cacagctaat cccagcaagg
     6421 aaggaatgag cagtaacccc attcgtttta ctccgggatg ccgcggatga aaattccagt
     6481 acaatgggaa accctgtttg tgcaaactgg gtaattgcat caggaattga gcgagccctc
     6541 ccactacaat gcctatggct acagccagaa tcgcctgatc aaataacggc gaaatggcca
     6601 ctgcaaaaaa aatgacacaa agattgaaaa agacaggagc caaggcagga acggcgaatg
     6661 aatggaggga attgagtatc cccatggcca gggccgcaag gcttacgaac agtaagtagg
     6721 ggaacatgat ctgcgtgagg agcgtggttg tagcaagttg atttgcctgc ccatggatgc
     6781 caggtgccag aacccagacg agacttggcg cggcaacgat gcccattaag gtgacaaagg
     6841 tcacgagtgt caggagtgtg gtgaatgctg cgctggctaa ctcccaggtt tcctgcttgg
     6901 agcgggtcga atgatattcg gtgaaaatcg ggataaaggc ggaagacatg gacccttccg
     6961 caaaaagttc tcgcaacaga ttgggaattc gatacgcgat gtaaaaggcg tctgctgaag
     7021 cgttggcgcc aaacaaattg gcgagaatga catctcggat aaatccgaga attcgactgc
     7081 agaaagtggc tacgccgatc agcccggccg cacgactaat ccgactggtc tctccttgag
     7141 gagatgacgg gactgatgga ggttgagctg attgggacat ccgaatagga atgataccgg
     7201 aaagggttga gttgtttcac ctgtcagatg tcaggggcga atgtcttgct ctatgataag
     7261 ggaacgctga atctcacgca gaatctggac gcgcacgttt tcgcttaggc ttgaattgac
     7321 accctctgaa tcatgcttta gcatcacttc ccttttgtct atgcacttct ccatcttatg
     7381 acaagatctt cgttgactca atccagtctg ccacgatggt ggtgttttgc gacggcgttt
     7441 tcgaccggtg cggtggtgat ggcgctggaa attttaggaa gccgactcct cgcgccggtt
     7501 tttggtagtt cactgtttgt ctggggcggg ctgataggag tggtcctggc tgccatgagc
     7561 agtgggtatg cggcgggagg ttggctggct gatcgatacg ctccgcagag ggttttggcc
     7621 gggctgttat tgctgtccgg tgtttggact cttttgttgt catgggcagg ccagcccgta
     7681 gtttttgggg tgtctcgatg gattcaggat cctcgttggg gtccctgttt tgccgccagt
     7741 gtgctgttgg cggttccggc atttggattg agcggggttc tcccggccct tttgcgtttg
     7801 gctatcgggg atataaggca tcttggacgt cataccggag gcatgattgc gatttcaact
     7861 attgggagtc ttgtcggaac ctgggggacc tcgttttatc tgttaacctg gctgggtagt
     7921 accacattag tcgctatcct tggcgtagtc cagatctgtc ttggtctatg gtggacattg
     7981 agagctggaa tcctccagcc tggtttacag gtcatactcc tttcctgtgt aggcggaata
     8041 acctggatgg gttttcatcc ggtcaccatt ttaccaccgg caatttatca ggaagatagc
     8101 ccgtaccaac aggtgagggt gagtgaaaat gatttattcc gttatcttat tttggacaat
     8161 actttccacg cggtgatgtg gaaagcgacg ccggaatcgc ttgccttgcc ctatagtcaa
     8221 gtcatgatgg gtgtgttgcc acttgttgag aatcctaagc gaggactgat cttaggccat
     8281 ggcggaggat cattggcaaa gtggttaggc aagcactggc cggatttgga gttagatgtt
     8341 gtcgaaatgg acccgtctgt cgtccaagcc gctgaacggt attttggtta tacgccaccg
     8401 ctcaaccatc atgtgtatgt ggaagacgct cgcacgtttt tacgaaacaa cccttaccag
     8461 tatgacgtgg tgtgggtcga tgtgtttgcc aggcatctca ttccgttcca tgtgacgact
     8521 catgaattct ttgaagaatt acggaagcat gtgaaccctg agggtgccgt tgccgtgaat
     8581 ctggcttcct ccgatgcgcc ggccaatgtt cgtcgcgcac aagcggtgtt gaccactatg
     8641 caacagacgt tccctgaagt ggtgccttac ggggttgcgg gccctgaatg gttagacact
     8701 gcaaaagggt ccgtgaacct gatttttttt ggtgggaaac ctgtctccgt aatgcgctct
     8761 tcgaactttt tggatgttct agcccgtcag atgaatcgtg accgatttcc gtcagaaggg
     8821 tttgcatttt tcatgtcaca ggagccggtc gtactggctc ctggagaaat tctgaccgat
     8881 gatttctctc cgctagattt acttcagggg gaagggtaac catccctttt gccttgagtc
     8941 agcagggttc ttcctttaat tccaaaatac agcgaaggat atccccagca ccttgctggg
     9001 gaagcttcac tagaaagtta acctattctt ggttttcatt gtgagaatgg caggtgttaa
     9061 aaaagggcaa gaggccactg taacaataaa tgggttccac tcatttggcg ttcggcgggg
     9121 actgatttgg cggtttttct ttcacacgtt taccaggtgt ccgtaagtac aacagtgggt
     9181 gcatgcaaga attgctggca tgagttgtcc cgtccttctt catcctggcc gtcgggaatg
     9241 gttcccgcgt tctcgccgtt atcctaaggc cgaagacaaa ggctgttgtg gagacaaaat
     9301 cgaaggggga aacattgttt aaaggtgctg gagtcccaga agcaacctta gttttgacag
     9361 acagcttctg gccaaaattt gtaacggatt tgtggtagct gagcgccgtc aaaattcggg
     9421 agatcataaa gacatcgatc aaccactgct acctcatctt gagtaaattc aaagactttc
     9481 cgtgttttcc aaaattgatc cagcgtatag tagtcccctg tggccggctt ctccgccagt
     9541 gcagcctgaa gtgctttgaa tactgcggta tcgcagccag ggatacgtcc attgttgcgg
     9601 gtaatgcacc atttaatgat ttcggctacc ccgtacatgc ttagatcaat tttttgcttt
     9661 tcctcagcca tggtttcctc cttacaaaca gacaaaaaga caagagatct ctataacata
     9721 aagagagtcg tggcatccag tgggctacgg tcatcccttt caaagtaact tttgacaaag
     9781 tctgtagctt cctagcaaaa acgagatttt tcggactact cgccggtttt acatcatttg
     9841 gaaaaatccg tatactcgat gcgctgtgca tgggttatcc tgcatctctt ttggtaccct
     9901 ctccacccac atattctgga gaaaggcaag aagaattgta tttcagtcag tccttgcgtg
     9961 ctcgacttgt cattcctctg gggcttttgg gtctgctgtc cttggtcggt tgtcctacac
    10021 gcgaggagta ccatcctcct gatttgattt atcattacct agacattcca gtggggaaaa
    10081 atcctacctc aatccgggca agtgatttta acggtgatgg atttacggat ttgatcacca
    10141 gcaatattca gggaaattcc ttgtccctgc ttttgggaaa aggggacggg agttttcagg
    10201 accaaaaaac gatttctgcc tgtcaggagc cgaggaatgt ggctgttgat gattttaatc
    10261 ttgaccacgt gcccgatttc gtcgtggcgt gttcggggga taatcatgct ctcgtgtttt
    10321 tggggcaggg cgatggcact tttttgcgtg gggcccaata cctggttcat cgtactccgg
    10381 tgagtattgc cacaggcgat tttaatgaag attttcttcc tgatttggcg gtggctctcc
    10441 gcaacgacaa aattcaaatc ctgttgggta tgggcaacgg aaaatttcga ttggggtcat
    10501 tatatgaata tggagatacg ccgacctctg tggctaccga ggatttgaat ggagataaac
    10561 atttggatct ggctgtgacc aatggagggc cgatgagtca tgctgtgtcg atctggttgg
    10621 gtttggggga tggcaccttc caagctccaa ctgactatcg aaccggcaag cggcccttgg
    10681 gggtctcttt tgcggacttc aataccgatc gggccctaga tcttctcgtg attaacggtg
    10741 agatgaacac gattaccgta tttttaggaa acggggatgg gacttttcaa gagggaagag
    10801 aatcaggagg cgatgccggt ccaaattttg gcgtggccca ggattttgac ggagataaat
    10861 tgccggatgt ggcggttgcc aatattcaat ccgggagtat ttcgttgcta tttgggaatg
    10921 gcgatgggac gtttcactat cctccaaggg attatcccac cccgcatgcc ccctttgcat
    10981 tgaccaccct attaattgcc accgatcggg gagaagaacc agggttggcg gtcgtgaata
    11041 atgcatctag taatgtctcc atttttttac atcatgggtt gaatctccgg aagagccaac
    11101 tttcgggaac tcccgcttct tgatacttcg aaggtttgtt cttgataccc gaattctgcc
    11161 atgtttacaa tactattgcc ttttagaatg aggcgtaacg ccgttccaaa gcgtggttca
    11221 gccttgtgag gagaatgtgc gaacttttgg ctggtggttt ggaatgggat ctctgctgac
    11281 cttggccgtc ttgctggttc aaggcggaat gagagacctg atttacggtt cttctcattc
    11341 cgggacgtgg gaaggaatac tttctatggg actgacggtt tttgggggcg gagtccttgc
    11401 gggatgtgtg gcggttattt taaatcgact tcgataggca aattggatgc ctcaggaaga
    11461 gggctgaaag ggactgtagt tattcagatt ggccctgggg aaaagaaaga agatgggtat
    11521 tatcccatcc gttcacgcgg gtgtctaatc agggaaatag gtttgtatcg atatgacgtg
    11581 tctcagatgc caaggaacga tgctggttga acgtcagtac accagggagt cgcgccggtt
    11641 caacgcgaaa tgtatcaatt gcggtttttg gtttgactta gcagatgtgt tgcgtttttt
    11701 taagcgagtc ctcgaaagcg gctacaacgg ccaaaaaatc cgtgaaaccc tttagccttt
    11761 ctgaatccaa cccaccgtac tttcagttct gcaacgttaa aatttttaag acctgatcgg
    11821 gtccttgtta ggattgtttg tggcgggcag gcgttggagt gaattccacg aattatccag
    11881 tggcctatta ggttttagtt tcttgtgcca aagtttaatg acggcagtat tgaaaaagtc
    11941 cgcccgcaga gttttcgccg cttggccgtg tccacatact nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn gcggaggcgg cttcttcttg gagaaagttg ataggttcca
    12121 ttgccgctgt tgagggtttg tgggaatccg aaatcatgat gcgattggtt ttgtctttta
    12181 actccaggac gattcgactc gccgattttt tcccaacgcc ggggatggaa cctaaggttt
    12241 caagatcgcc ggtttcaatg gccttacata aatctgggac ggaaagggtt gataacgcac
    12301 tcagtgcgag tttcggtccg acgccgctga tagtagtgag gagcgaaaag gcctgcttct
    12361 cctcggtggt ggaaaaccca aaaagttgaa tggtgtcgtt ccgcaggtga gtggaaacaa
    12421 aaacctggac tgcagaattg atttcaggaa gagtgaaata ggtagaaagg gctatgaaca
    12481 gatgataccc cacaccctgc acagaaatga cggcatcttg tggcgtctta gaagagagga
    12541 ctcccgaaag tgaggcaatc attttggcag actcagttgt tcgtcaaagt ggaaggtgac
    12601 aggagaattc aagatcaatg agagaaagcg gtaagagaaa aagatcagag aatggagtgg
    12661 gatcaaaagg gctaggtgct ttctgcagcc attttctcca tcagttcgtc agatatctca
    12721 aaattggcat ggaccttgga tacatcttcg ttctcgtcta ggagatccat gagcttcaag
    12781 gcttgttctg catctctgcc ttcaagttga atatggtttt gcgcgaggaa agtgagctcg
    12841 gacaggctgg tttcaatctg ggccttctgt aaggcttcct tgacggcttc gaaatttgct
    12901 ggttctgaaa tgacctcaaa tccggtggga gtttgtttca cgtcttcagc tcccgcttcc
    12961 agcgccagat caaatagttg ttcttcagtc accgcctggt tttcgatgac cagtaacccc
    13021 tttttttgaa attgccaggc aaccgcgccg gcttcagcca tcgttccatt attttttgtg
    13081 agaatatttc gaacttcggc tacggtccga ttgcgtttat cggtcgaaat ctccaggagg
    13141 agtgccgtcc ctcctggtcc atacccctct agcataaact cctcgaattg cattcccgga
    13201 agttctccag tacctcgctg aatagcccgt ttgaccgtat cggcgggcat attgacttct
    13261 ttggatttcg caatggcttg gcgtaaggct ggattcccgt ctgggtcgcc gccgctacgt
    13321 gcggcaatgg aaatttcacg gatgacgcgc gtgaagattt tcccacgctt ggcatcctgt
    13381 gcgcctttgt ggcgcttaat tgttgaccaa tggctatgtc cacccatggt tgcggtccat
    13441 ttcctaaaat taaggggtca agaatattat aggtgttata gtaagccccc gggtgggctt
    13501 ctatcacagt aaatgaaagg gagtcaatgc acgcgggcat ggcaaaagaa aggttggcaa
    13561 agcagacggg atgttattgt cctggagagt caacaagctt tattctgtgc atttgatttt
    13621 cctctttttc cagtgttaag gaaattagtc atgcgcattg tatttatggg aactcccgcc
    13681 tttgcagttc ctacgcttca acagttattg aaaactgaat tttctgtcgt gggtgttgtg
    13741 tgtcagcctg accgaccgag tgggcgtggg aagaaagttc aagtcggccc ggtaaagggg
    13801 cttgcattgt cacaaaatat cccggtggtg cagcctgaaa aaatgaagga tcccaaattg
    13861 atggagactc ttcgagcatg ggaacctgat ctggtggtgg ttgccgcgtt tgggcgtatt
    13921 ttgccaaaaa ctattcttga gctccccccg aaagggtgtc tgaatgttca tgggtccctc
    13981 cttccgaagt atcggggtgc tgctcccatt caatgggctg tgatcgatgg agaggcggag
    14041 accggtatta cgatcatgct catggatgaa gggatggata cgggggccat tctacagcag
    14101 gaagtgattt ctataggtca ggacgagact tccggagagt tagcttcacg aatggctgag
    14161 attggcggag cgcttctggt ccggactctg cgtggatggg tcgctggcac agtgactcct
    14221 tcggctcaag atgattcggc agcgaccttg gcgcccatct taaccaaaga agatggcttg
    14281 atgtcttggg gacaatcggc gcgatgcctt gccaatcgta ttcgggggct gtcgccatgg
    14341 cctggtgtgt ataccttcct ggagggtgag cgatggggaa tttggaaagt tcagattgaa
    14401 gaccaagaat caggtatatt gcatactact catgacgctc ctcaggctcc aggaacaatt
    14461 actgccatca cgaaacagtc catccgcgtc cagactggtc agggaagttt gcatcttttg
    14521 gagattcaac ctgaaaacaa aaaacgcatg cacgtttctg actatttagc cgggcatcgg
    14581 attacactcg gtatgacctt ctcaatgggg cagcaagatg attgtgcagt gtaatcgtgt
    14641 ggttaaaata aaaaaaagtg tggcaccggc agcctggaat tgccaggcgt tttcgtatga
    14701 cccgttttgg tggaaaagga tccaataatg accgatcaat cctccgagct attagcgttt
    14761 atacaaagtc aaatcgagga aattaacacc attcacgcac aggcggagaa ggccctcaat
    14821 gcggtgcagg ggaaagacca tgtcacgaaa tggaaaagaa aggtcgttga aggcctggca
    14881 ccctacgtga gtcagacata tcttcaactc attaccaagg aatggctgga aaccacctat
    14941 tttgtgggcg atgtgtttga cgaattagcc gatgaagtgg atatgtgtcg gcgccatctt
    15001 aagaagttgc ttaaagacat tcagaccacc ggcatcccct gatgccactt accctatgaa
    15061 tcaccggtat gcctcttctc atttttctcc ttgcctataa tgatggcacc gcttcctcct
    15121 tatccgcgca aacggctcgg tcaacatttc cttattgacc ccaatttact ccgaaaaatt
    15181 atcgatctgg cggatctcca gccgaatgat tatgtctgtg aaattggacc ggggggcggc
    15241 gccttaacca gattgttgtg ccaatcggca aggcaggtgt tagccctgga agtcgatccc
    15301 cgtatggtgg attttttgaa agaggaattt tcgggatatt cgaatcttga tattcgagaa
    15361 cacgatgcgc ttcgttatcc cttcgatcaa ctaccggaac ctgctgtcgt agtggccaat
    15421 ctgccctata atatttcaac ccccattatg tttcggttat taggagcccg gccaaagatc
    15481 cgtcggatgg ttttaactgt acagctggaa gtcgcaaaac ggttaaccgc caaccccaat
    15541 actaaagact atggggtgct ctcggttatg gcgcagcgtt tagctgatgt ccgtttcggt
    15601 ttccccgtgt ctcgaaaatg ctttcgcccc gttcctg
//
LOCUS       DAORAQ010000057        15496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_648_length_15496_cov_70.412927, whole genome shotgun sequence.
ACCESSION   DAORAQ010000057 DAORAQ010000000
VERSION     DAORAQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15496
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_648_length_15496_cov_70.412927"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..497
                     /locus_tag="PKM72_07110"
     CDS             165..497
                     /locus_tag="PKM72_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNP60593.1"
                     /translation="MGSKIYVGGLPYSTAEQELADLFGQHGAVTSAKIITDKYTGQSR
                     GFGFVEMASDAEAKAAIEALNGTEMGGRTLTVNEAKPQAPRTGFGGGGGGGNRGGGGN
                     RGGGGDRW"
     gene            698..2479
                     /locus_tag="PKM72_07115"
     CDS             698..2479
                     /locus_tag="PKM72_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP60594.1"
                     /translation="MSDSSEFSSPKDSSPSGGSSFSKSSRQSGISPLALIDQCLASLP
                     EGDQRIPLLYQLRHRLVEQTTASQQQEADMKKLQGVLEKLTAPANRIGTLLGQPDEGV
                     ARIMVGGAEYYANVDPRLERDGLKVGHQILVNEAYVVIRSLGYDRSGPIVKVREILGD
                     GRLRIDQEVGRQGILIQRAEELASADLKVGDEVRLDPTHKIAIERLENSQGQSHLLAE
                     LPTVRWEHIGGQQEAIGAIRRSIEYPLLHEGLFSRYQFQQPKGFLLYGPPGCGKTLIG
                     QATAASLGQILAQERETPTPHVGTQGQDSLSQLPPIEGGVFLHVKGPEVLNMWVGESE
                     RIIRDLFLQARQQRLAGKLPFIFIDEAESILGTRRAVRSYNVSNTLVPMFCAELDGIE
                     SLSQVVVILASNRPDMIDPAVLRPGRIDRKIKVGRPGRNDVGEILRVYLTADLPYDTK
                     KSAGSGEDDGTPVSRIIDGLLENLFQRSQENRVLAIRLRNGRREILYRRDLLSGAVLA
                     GIVRRAKERAIERAIQAGDQEPSGLTLDDLLIATNEEFKESEIFPPDDSAEEWLKLLD
                     YHPDQVVGVSSLQPGKDGEEHRTSQIV"
     gene            2537..4060
                     /locus_tag="PKM72_07120"
     CDS             2537..4060
                     /locus_tag="PKM72_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNP60595.1"
                     /translation="MNPIRLFGIETEYGIAREDVETADPVVESMELVRAYLDGHFTRR
                     WDYQGEHPHEDQRGFRVTELAQDKEEDLFAEQDAHRPFSFHEMKSDLVLPNGARFYND
                     HTHPEYSTPECRSLKDMVAHDRAGERILWMAAQRRNKVLGGPMVQLYKNNTDFHGHSY
                     GCHDNYLVSRTLNFENLARGLMPFLVSRQLIAGAGKVGREAQERGFVPGGFQLSQRAD
                     FMETELGVDTMHNRPILNTRDEPHANRERYRRLHLIVGDANMCEYATALKIGTTRVVL
                     DMIAGGLVPPCELDSPVQAIQALSLDFELNTLVKRKTGGMISGLDIQREYLNLARKFF
                     IEMDEETKWILCEWEHVLDLLERDRLQLVGKIDWVTKWWLLETFSQAEAVGWDDPWLA
                     SLDLEYHNLDPDRGLFFGLESEGRTTPLCQEQEIQKAMKHGPLDTRGGLRGLCVQRFE
                     KDIASIQWEGVHFRGQNGGLHLDLQDHLEPVSVARCRSVIEYAATPLDMMKALTGEQ"
     gene            4290..5024
                     /locus_tag="PKM72_07125"
     CDS             4290..5024
                     /locus_tag="PKM72_07125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP60596.1"
                     /translation="MPNSFNFLERNRVVRLTDGGFWLSDEKKVYEYQRFTALIQDLQR
                     KKIKILDVGCGKGRIPRKLAEDSHINVEFEYSGFDVDRNDINENIAFFQSDNRFTFNH
                     IDYMNKMYSPNASTKEPVFDFLDDSYDLVWAWSVFSHLDIKDFEMYMNLISDKLVRNG
                     FAFFTCFSRYEAKRNPEFTPNYIFLENSDLPANGGKQDNNNLTAVCYNPSWIDELLMK
                     LGLEVIMSCYPVYGGNFQTGMYLRKV"
     gene            5074..5286
                     /locus_tag="PKM72_07130"
     CDS             5074..5286
                     /locus_tag="PKM72_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HNP60597.1"
                     /translation="MENTYAYIQTGGLERKEQPIDPLSKPSGPGDQESGPKKPETDSP
                     SRDNLMKRMRKVDPKQAEKYRQRTGE"
     gene            5288..6079
                     /locus_tag="PKM72_07135"
     CDS             5288..6079
                     /locus_tag="PKM72_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNP60598.1"
                     /translation="MGQQGDFIQILRERGYAVDDLCQPFNVNGPSAHVDITTGTTVFA
                     IKYADGVLVAGDRRATAGNVVMYDRTDKVLELDRFSVMAIAGVPATAFEMARILEHSF
                     KYYRRSQLQDLSFEGKLRALSTLLKNNAPAALAGTGTVAPIFAGYDQLEGCGKIFFYD
                     ILGAEFEAVEYAVSGSGSSTIRGILFYMNRWGSGPVAGMNEEDALTFVLRLMTSAAEF
                     DSATGGVDAQAQLYPVVKRISPEGVRTIPAERLKSLFSEKVLNHV"
     gene            6072..6890
                     /locus_tag="PKM72_07140"
     CDS             6072..6890
                     /locus_tag="PKM72_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60599.1"
                     /translation="MYEEPYRWIEAVANRRNYLDDQFTQGNPLVGVSFAQGILLLTFN
                     RGTEKIYEIYDRIALGGMGHPADLEKLRYTLLDMAHVEGFQRSPADVTASRLMKNGLA
                     PVIKQAFEEIFKAPYIAKILLAEVGATQDRDRLLSINYDGMFEEQREFGLLAASPSIR
                     GCLTQEWKNHSPLSSLPSAVEVSLRLWGIASHLQTSTPSSDSEEVASTSSAATSMSTI
                     PDDEAIASRIRETLSKEALECVLLDRESGKMETYRVLSQSELQSFIPPVFQPAT"
     gene            6912..8444
                     /locus_tag="PKM72_07145"
     CDS             6912..8444
                     /locus_tag="PKM72_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNP60600.1"
                     /translation="MHNRIFGIETEYGLLIKADQPDVSPSWVARRIIEYLFGQKKHGV
                     IDWHYRGHDEPPGNGGFLLNAGRMYVDMGHLEYASPECHSLWDLVAVDRAGDWLLQDC
                     LDELGLSELVSIIKNNIDHETDATFGCHENYLVTRDFPFTYKGLGLLMPFLVTRQVFT
                     GAGRIGMARSVDGWINLDDIEGRGRDRSRGSAAEGVLYQISQRPDYIVNDFFEWVQHN
                     RAIVNTRDEPLADPERFRRLHLLMGDSNMAEEATLLKMGTTGLVLQLIEEGHAPQGLD
                     FDDPVQTLRAISRDPQQKWMVTLSTGQHLSALDIQEQFWEAADKHYAGQDEETDWVLA
                     HWESVVKDLHKGYQEVVGRVDWASKLSLLETFREAEKLEWDDPWLKSLDLEYHNVDPQ
                     AGLYHGMAEEGDAPRFTTNDLVELAKGHPPRNTRAFGRSEIIRHIVKEGWADFLEKAT
                     GHHDPRRPPYVINWSVLQLQGSPAFVMADPFRSYVQESRDYCDEWSSRPSMESLPNKP
                     DI"
     gene            9693..10694
                     /locus_tag="PKM72_07150"
     CDS             9693..10694
                     /locus_tag="PKM72_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP60601.1"
                     /translation="MIYPVVEDAEYSSKYVAQIQSVNYVEIRSKIRGYIEKIYVDEGQ
                     PVKKGQLLFALTNNVFEKELQKANAAYRSALADLKVAKVELANVTVLVEKNIVSKSEL
                     YVIQAKVDALKADVEEAMANKEQEALNLSFSKIRAPFDGFINRIPNKVGSLIAEGDIL
                     TSLSDNREVFAYFNLSEVDYLNYIASGERGTKVVSFELANQVLYGYEGTIEMIESEFD
                     SSTGNIAFRARFPNPDGLLKQGANGKVIVHKILENALLIPQKSTFEIQDQLYVYVVNQ
                     DNVLEQRNIVSKMRLPNLYVVESGLSEDEQILFEGVENVMNGDKVHPVHVDIATAML"
     gene            10726..13935
                     /locus_tag="PKM72_07155"
     CDS             10726..13935
                     /locus_tag="PKM72_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP60602.1"
                     /translation="MTAIFIHRPVLSIVISLFITLLGLLSFTQLPVTQFPDITPPEVN
                     VTSKFIGANAEAVVKAVVTPLERAINGVAGMAYMASVSGNDGTSLIQIIFKAGTDPEI
                     AAVNVQNRVASALEELPPEAIKSGVIVEKVQNSMLLYINILSLDPTIDEKFLYNFTDI
                     NVLKELKRIDGVGFAEILGSREYAMRVWLRPNKLVMYNISALEVIEKLQAQNVEAAPG
                     KIGESSGRNKNAQALQYVLKYTGKHNTKEAYEDLVLSSTDDGEILRLKDVADIEFDSQ
                     DYDVLSKENGQASAAIVLKQRPGSNAKEVINTIKEKMEEIKSTSFPPGMDYSLSYDVS
                     QFLDASIHQVLTTLVEAFLLVSLVVFVFLQNVRLTIIPILAVPVSLVGTFFFMHMMGF
                     SLNLITLFALVLAIGIVVDNAIVVIEAVHAKMEGLGIGAQQATEEAMHEISGAIMAIT
                     LVMSAVFLPVAFMDGPTGIFYRQFSLTMACSIVLSGITALTLTPALCAVFLKNTHNSE
                     HPQPSRLQKLFDGFNRWYDNLSDNYKKLISAIGNRRIITFGVLLGFSVGTGLIGAFVP
                     SGFIPNEDQGTIYANVTAPTGATLERTEKVVDEVQQIASELEDVTSVSSLAGFSILSE
                     GTGAVYGMNLISLKKWNERMASDNEIISLLEEKTKHIKDASIEFFTPPPVPGYGNSSG
                     FEMRLLDKTGSGTLEELQKTADSFVDDLNKRPEIANAFTTFNARFPQFMLHIDGEKAA
                     QKGVTAENAMNTLQALIGSVYATNFIRFGQMYKVMVQALPEFRAEPDDLMKLYIKNDT
                     GEMVHFSSFLRVEKIYGPEQVTRYNMYPSAMINGQPAKGYSSGQAITAIKEVAASKLP
                     KGFGYDWAGSSRDEAQMGNQAIYIFLICLLFVYLLLAAQYESFLLPMPVILSLPVGIF
                     GALFFLLIMGLENNIYAQIAMIMLIGILGKNAILIIEFAILQQKRGKTAFEAAIEGAA
                     LRLRPILMTSFAFIAGLLPLMFASGAGEIGNNTIGSAAAGGMFFGTIFGVILIPGLYV
                     VFATMGESKNREERSKTGKASRLSFKSRFKLARRLAKLQ"
     gene            14011..15429
                     /locus_tag="PKM72_07160"
     CDS             14011..15429
                     /locus_tag="PKM72_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP60603.1"
                     /translation="MYSFLIILFLALIFNGCTGLKTDLAIQENEIPTSFKDKTDTTNI
                     ADIQWKDYFVDPLLHELIETAIENNLDLQMALQRIETSRSSVRAATGALMPTMGLGVG
                     TAIRRYGLYTMDGAGNATTDFKSGQQIPDNLPDLFVGLQSSWEVDIWGKLQNQRSSAM
                     AKFLASIEGTSFVVSNLVADVAIYYNELQALDNELDIIRQTLHTQYESLEVVELQKEA
                     GRATELAVELFKAQVLNTSILEKEVLQQITITENHINFLLGRYPQPIERTKGQYFSEI
                     PHEISSGVPSQLLANRPDVREAELQIKGSNFDLKVAKAAFFPSFNITASFGFQAFNPD
                     FLFVAPASIAYTAVGTLVAPLINRNALQAQFNNAEANQLTAMYHYQKTILNAYVEVAN
                     ELSNIHNLRKINILKKKQNEVLRQSVETSHLLYRYGRATYLEVLTAQRNTLQSDLELI
                     NFAKQLRIAAVKIYKALGGGWK"
BASE COUNT         4028 a         3312 c         3995 g         4161 t
ORIGIN      
        1 agtgacgggc ccatcactga tgttgttttg ccaaggaatc cgcaaggagt ctgattggaa
       61 gttagtccta agtcgccatc tggtttgcgt tctcgggccg ttccttccct tctcttctct
      121 tcgaaccatg caaaacaata tcgcatttag aaggagggac cccgatgggt tcaaagatct
      181 atgtgggtgg cttgccgtat tccacggcag agcaggaatt ggctgacctt tttgggcagc
      241 acggcgccgt gacgtcggct aaaattatta cggacaaata caccgggcag tctcggggat
      301 ttggttttgt ggaaatggcc tcggatgctg aagccaaggc cgcgatcgag gcgctgaatg
      361 ggaccgaaat gggagggcgt accctgacgg tcaatgaagc gaagcctcaa gcgccacgaa
      421 cgggatttgg tggtggcggt ggtggcggaa atcgtggcgg cggaggaaac cgtggtggcg
      481 gtggagaccg ttggtaacgc actaagtcac taaaggttac actttaatag aagcagggga
      541 tccggtattg gatcctctgc tttttttata ccttgggtaa tggccaggaa acattgtaga
      601 atgatttgac aactgccgga caagatgact cggcaatggt ttagtgtgca tcacggcttt
      661 catcttccaa ctgaaataga atggaaagat atatacgatg agcgattctt cagaattttc
      721 ttcccccaag gattcttctc cgtcaggtgg ttcctcattt tcaaaatcct cccgtcagtc
      781 gggaatttcg ccgttagcac tcattgacca atgtttggca tcattgccgg aaggggatca
      841 acgcattccc ttgctctatc aacttcgaca ccggcttgtt gaacaaacga cggcttctca
      901 acaacaggaa gccgacatga aaaaactcca gggggtgctt gaaaagctga cggccccggc
      961 aaatcgaatc gggacgttgt tgggtcagcc tgatgagggc gtagccagaa ttatggttgg
     1021 tggggcggaa tactatgcca atgtcgatcc caggctcgag cgagatggct taaaggttgg
     1081 acatcagatt ttagtgaatg aggcatatgt cgtcatccgg tctcttgggt atgatcggag
     1141 tggtccaatt gtgaaagttc gagagatttt aggcgatggc cgtcttcgaa ttgatcaaga
     1201 ggtcggcaga caaggcattc tcattcaacg ggcggaggag cttgcaagtg cggacctgaa
     1261 ggttggcgat gaggttcgat tggacccgac tcacaaaatt gccattgaac gtttagaaaa
     1321 ttcacaaggc caatcgcatc ttttagcgga gttaccaact gtgcggtggg agcatattgg
     1381 tggccagcag gaagccattg gggccattcg ccggtcaata gaatatccgc tcctgcatga
     1441 agggttgttc tcccgttatc aattccagca accaaagggt ttcctacttt atggcccacc
     1501 cggctgcgga aaaacgctga tcggccaggc tacggctgcg agccttggac aaatcttagc
     1561 gcaagaacgt gaaacgccca cgcctcatgt aggaacccag ggacaggatt ctctctcgca
     1621 acttccgccg attgaaggtg gcgtgtttct gcatgtcaaa ggtcctgaag ttttgaatat
     1681 gtgggtcggg gaatcggaac gaatcatccg ggatttattt ctccaagccc ggcaacaacg
     1741 actggccgga aagttaccgt ttatttttat cgatgaagcc gaatctattt tagggacgag
     1801 acgggcagtg cgatcctaca atgtctccaa cacgctggtt ccgatgtttt gcgctgaact
     1861 cgacgggatt gaaagccttt ctcaggtggt cgtgattttg gcctcgaatc gtccggatat
     1921 gattgatccg gcggtgcttc gacctggaag aatcgatcga aaaatcaaag ttggccgacc
     1981 tggaagaaac gatgttgggg aaattttgcg tgtctatttg accgctgatc ttccttacga
     2041 cacgaagaaa tctgccggca gcggagaaga tgatgggact ccggtttcgc gtatcatcga
     2101 cggactgtta gagaatctct ttcagcggag ccaggagaat cgcgtgctgg ctatccggtt
     2161 gcgtaacggc cggcgggaaa ttctctaccg gcgggattta ttgagtggag cggtgttggc
     2221 gggaatcgtc cggcgtgcca aagaacgggc cattgaacgg gcgattcaag cgggtgacca
     2281 agaaccatcg gggctgaccc tcgatgattt actcattgcc accaatgaag aattcaagga
     2341 gagtgaaatt tttccccctg atgattcagc tgaggaatgg ttaaaactct tggattacca
     2401 tccagaccag gttgttggag tgtcgtccct tcagcctgga aaagacggag aagaacatcg
     2461 gacctctcaa attgtttaaa cctccctttc taacaagctc tcaaatggga cattctcagt
     2521 tgaaagatcg gaagatatga acccgattcg cctgtttggc attgagaccg agtatgggat
     2581 tgcgagggaa gatgtcgaga ccgcggatcc tgtggtggaa tccatggagt tggtgcgggc
     2641 ctatcttgac ggacatttta cccgccgttg ggattatcag ggcgaacatc cccatgaaga
     2701 tcaacggggg tttcgtgtca ccgagttagc tcaggataaa gaagaggacc tgtttgcgga
     2761 gcaggatgcc catcggcctt tttcctttca tgaaatgaaa agtgaccttg tcctccccaa
     2821 tggcgcacga ttttataatg accatactca tccggaatat tccacgccgg aatgccggtc
     2881 tcttaaggat atggtcgcgc atgaccgggc tggcgaacga atcctttgga tggcggcaca
     2941 acgccgaaac aaggtccttg gcggaccgat ggtccagctg tataaaaaca acacggattt
     3001 tcatgggcat agttatggtt gccatgataa ttatttggtc tcgcgcacac tcaatttcga
     3061 gaatttggct cgcgggctca tgcctttcct ggttagccga caattgattg ccggcgcagg
     3121 caaggtcggt cgtgaagcgc aagagcgcgg gtttgtgccc ggcgggtttc aattgtccca
     3181 acgcgcagat tttatggaga ccgagttggg ggtggatacc atgcacaacc gccccattct
     3241 taatacacga gacgaacccc atgcgaatcg cgagcgctat cgacgtctcc atctcatcgt
     3301 gggcgatgcg aatatgtgtg agtatgccac cgccttgaag ataggtacga cacgcgtggt
     3361 gttagatatg attgcgggtg ggctcgtgcc gccctgtgaa ttagactccc cggtgcaggc
     3421 gattcaggcg ctgtcgcttg attttgaatt gaacacgctt gtgaagcgga aaaccggcgg
     3481 catgatttcg gggttggata ttcaacggga atatctaaat ttggccagaa agttcttcat
     3541 cgaaatggat gaagagacga agtggattct ttgcgaatgg gagcatgtgt tagaccttct
     3601 cgagcgggat cgattgcagc ttgtggggaa aatcgattgg gtgacaaaat ggtggttact
     3661 cgaaaccttt agccaggccg aggccgtcgg ctgggacgac ccttggcttg ccagtctgga
     3721 tctggaatat cataatctgg atcctgatcg cggcctgttc ttcgggttgg aatccgaagg
     3781 tcgaacaacc ccgctatgtc aggaacaaga gattcagaaa gccatgaagc atggaccact
     3841 cgatacgcgt ggggggctcc gtggattgtg tgtccaacga tttgaaaaag acattgcgtc
     3901 gatacaatgg gaaggggttc attttcgtgg ccaaaatggg ggactgcatc ttgatctgca
     3961 ggatcacctt gagccggtgt cagtggctcg ctgtcggtct gtaattgaat atgccgcgac
     4021 tccattggat atgatgaaag ctctgacagg ggagcagtaa ctttaggtgg gattattcta
     4081 atttttggat attttacttc accttagcag aatatttagc aaaaatttgg agtgattata
     4141 ctcccggtta ggaataatac ctccatgaat tggttggtaa tctttctagc gaacctactg
     4201 atggcggggg aaagtcgtcc ccgaacttca ccggatgaaa aagggaattt tgagaattgt
     4261 tagttgatct aagaaccaat aagggagtca tgcctaactc ctttaacttc cttgaaagaa
     4321 atagagttgt tagactcact gatggcgggt tctggctttc tgatgaaaaa aaagtatacg
     4381 agtatcaacg gttcaccgca ctcattcagg accttcagag aaaaaagata aaaatacttg
     4441 atgttggatg tgggaagggg cgcataccaa gaaaattggc tgaagattca catatcaatg
     4501 tcgagttcga atattctggt tttgatgtgg atcgtaatga cattaatgaa aacattgcgt
     4561 tctttcagtc ggataaccgg ttcacattta accatattga ttatatgaat aaaatgtaca
     4621 gcccaaatgc ttcgacaaaa gaacccgttt ttgacttttt agatgattca tatgatttgg
     4681 tttgggcatg gagcgttttt tcccatttgg acattaaaga ttttgaaatg tatatgaacc
     4741 ttatttctga caaattggta agaaatggtt ttgccttttt tacctgtttt agtcgatatg
     4801 aggcaaagcg caatccggaa tttacgccaa attatatttt tttggagaat agtgatttac
     4861 ctgctaacgg tgggaagcaa gataataata atttaactgc cgtttgctac aaccccagtt
     4921 ggattgatga gttgttaatg aagctaggat tagaggtcat tatgtcctgc tatcctgtat
     4981 atggtgggaa ttttcaaaca ggtatgtatt tacgtaaagt ttaatgtatg ttgttctgga
     5041 aagcatgaaa agttcatgtg gaggatgaga tttatggaaa acacatatgc atatatccaa
     5101 accggaggcc ttgaacgaaa agagcagcca atcgatccac tctcaaagcc atctggacct
     5161 ggtgatcagg aatcaggtcc taaaaagccg gaaactgatt ctccttcccg tgataatctc
     5221 atgaagcgca tgcgcaaggt ggatcccaag caggcggaaa aatatcgcca acggaccggg
     5281 gaataagatg ggacaacagg gagattttat tcagatttta cgagaacgtg gctatgcggt
     5341 tgacgatctt tgtcagccgt tcaatgtcaa cgggccatca gcccatgttg atattacaac
     5401 cggtacgacg gtctttgcca tcaagtatgc agatggtgtc ctcgtagctg gtgaccggcg
     5461 ggcaacggcc ggcaatgtcg tgatgtatga tcggactgat aaggtcttgg aattggatcg
     5521 tttttcggtc atggccattg caggtgttcc tgccaccgcg tttgaaatgg caagaatctt
     5581 agagcattct tttaagtatt accggagaag ccaattgcag gatttgagtt ttgagggaaa
     5641 gttgcgagcc ttatcgacat tgttgaaaaa caatgcgcct gcggcgttgg caggaaccgg
     5701 aaccgtggcg ccaatttttg cggggtatga tcaacttgag ggatgcggga agatcttttt
     5761 ctacgacatc ttaggagccg aattcgaggc ggtagaatat gccgtgtcag gatcaggatc
     5821 ttctacgatt cgggggattt tgttttacat gaatcggtgg gggagtggtc ccgtggctgg
     5881 gatgaatgaa gaagacgccc tgacgttcgt gcttcggcta atgaccagtg ctgcagagtt
     5941 tgattctgcc accggtggag tggacgcgca ggctcaattg taccccgtcg tcaaacgcat
     6001 ttcccctgaa ggagtgagga ccattcccgc cgaacggttg aaatcgctgt tttcagaaaa
     6061 ggtgctgaat catgtatgaa gaaccctatc gatggattga agctgtcgcc aatcgacgaa
     6121 attatctgga cgatcaattc acacaaggca atccattagt cggtgtttcc tttgcccaag
     6181 gaattctgtt gctgaccttt aatcggggta cggaaaagat ctatgaaatt tacgatcgca
     6241 ttgcgctagg aggcatgggg cacccggctg atctggagaa attacggtat accctcttgg
     6301 atatggccca cgtggaaggg tttcaacggt cacccgcgga tgtgaccgct tcccgcctga
     6361 tgaaaaatgg tctggcccct gtcattaagc aagcttttga agaaattttc aaggcaccct
     6421 acattgccaa aattttgctg gcagaagtcg gggctaccca ggatcgcgac cgcttgctca
     6481 gcatcaatta tgatggcatg tttgaggagc aacgcgaatt cgggttgctg gcggcttccc
     6541 cgtcaatccg tgggtgtctt actcaggaat ggaaaaacca tagtcctctc tcttctttgc
     6601 cgtcggcggt ggaggtgagt ctccggctgt gggggattgc gagtcacctc caaacctcga
     6661 ccccctcttc ggattccgaa gaggtggcaa gcacctcttc ggcagccact tccatgtcca
     6721 ccattccaga cgatgaggcc attgcttcac gaattcgtga aacgctctcc aaggaagcac
     6781 tggaatgtgt attgctggat cgggagagcg ggaagatgga aacctatcgt gttctgagtc
     6841 agtcggaatt acagtccttc attcctcccg tgttccagcc tgccacctaa tcctggcaga
     6901 gtgagattcc tatgcataat cgaatcttcg gcattgaaac cgaatatggc ctgctcatta
     6961 aggccgatca accggacgtg tctccttcct gggtcgcacg ccggatcatt gaatatctgt
     7021 ttggccagaa aaaacatggc gtgattgatt ggcattatcg tgggcatgat gaacctccag
     7081 gcaatggagg ctttttgctc aacgcaggcc ggatgtatgt cgatatggga catcttgaat
     7141 atgcctcgcc ggaatgccat tcgttgtggg atctcgtggc tgtggaccgt gccggagatt
     7201 ggctattgca ggactgtctg gatgaactag ggctttctga gttggtgtcg atcataaaaa
     7261 ataatattga tcatgagaca gacgcgacat ttggctgcca tgaaaactac ctggtcacgc
     7321 gagattttcc ctttacttat aaagggttag ggttactaat gccttttctg gtgacacgcc
     7381 aggtctttac cggggccggc cgaatcggga tggcacgttc cgtggatggc tggattaacc
     7441 tggacgatat tgaagggcgt gggagagacc ggtcacgggg atcagcggca gagggggtgc
     7501 tctatcaaat ttcccagcgt ccggattaca tcgtcaacga tttttttgaa tgggtccaac
     7561 acaaccgggc cattgtaaat acccgggatg agcccttggc cgaccccgaa cggttccggc
     7621 ggttgcattt gctcatgggc gattcgaaca tggcagaaga ggccacccta ttgaaaatgg
     7681 gtacaaccgg attggtcctg caattaatcg aagaagggca tgccccacaa gggttggatt
     7741 tcgatgatcc tgtccagacc ttgcgagcca tctcccgcga ccctcagcaa aaatggatgg
     7801 tcactctctc aaccggccag cacctttctg ccttggatat ccaggagcaa ttttgggaag
     7861 ccgctgataa acactatgcc gggcaggatg aggaaacaga ttgggttctg gcccattggg
     7921 aatcggtcgt gaaggattta cacaaagggt atcaggaggt ggtgggccgg gttgattggg
     7981 cctccaagct ctcgttgctg gaaacctttc gggaagcaga gaaattggaa tgggacgatc
     8041 cctggttgaa aagtttggat ttggaatatc acaatgtcga tccacaggca gggctgtacc
     8101 atggcatggc agaagaaggc gatgcgccgc gctttacgac gaatgacctg gtcgagttgg
     8161 cgaaaggtca tccaccacga aatactcgtg cgttcgggcg atcagaaatc attcgccata
     8221 tcgttaagga agggtgggca gatttcttag agaaggcgac aggccatcat gatccgcgcc
     8281 ggccacctta tgtcatcaat tggtcggttt tgcaacttca ggggagtccg gcttttgtca
     8341 tggccgatcc ctttcgcagc tatgtgcagg aaagccgcga ttattgcgat gaatggtcct
     8401 ctcgcccttc catggaatct ctccctaata aaccagatat ctaaagctcg gaccgagtat
     8461 gccggtggca ccatttagtc tgccgtttat taagtgttga cgcctttact gcattattcg
     8521 acagtgataa cttaaactaa gtaagggaag gcaagcaggc gaatgtcaag ggcgttatca
     8581 aaatttttta acgaaatcag ttctaggcta gctgatcctt caggatagga actccaggta
     8641 ataaaacaat atgtctcaaa tatcggtttt tcgtggttta gtggaggata gcgtgataga
     8701 tcgtggcatc ctgacatgta aggatcattt ttcaaatggc gccctataat taccaccaat
     8761 ttattggaag tttttagccc gtctcgaagg tgagggctgt ggctcataat ttgctctctt
     8821 ttttaggaaa ggatgaaatc cctaggtatg gttctgtcca gtttggtctt gaaagccata
     8881 acattaaatg tccatggagt ttatagccat ggtagttttg acttcgcacg aatgaaacca
     8941 atttgtggga atgtcccatc cgcacgatgt caggaagtcg aggctgcaac aacgataact
     9001 gagagctttg tttttatgaa atgaattggg ataggtttat tttgtgatta agggagggag
     9061 gaccaaattc tcagagaaaa taggtagctg gcaatcttga tgaaatttat ggcgcctgcg
     9121 ttatgtcctc aatcgggctt tatttcattt ccatgaaaac ccgttgtctg tcattgcaga
     9181 gaacatttgg actcacgttg aggaaagcca gtgtcggtgc ggaaaaaatc ctaggtttga
     9241 tctatggtgt tcttggggat gctgattgtc gattgtgtat ccctacctgc actcaaatcg
     9301 gatttgtttt gatttgagga agtgtatttt ttctttctgt gctttccata tctgtaaggc
     9361 tgtacccaaa atatatttac gcataggttt tttagttatg gggtgtcgga ctcctccact
     9421 tgtttcactc aactggattg ggctctgttt ctccctatga gtaggcgtta tttcgtgtgt
     9481 aggctcaatt tctttgcact gaagagtttc tctggttggg aaccccatcg gtatttgatc
     9541 gaatcaagag agctcaattc gttccattgt tcacgatttc acatagaaga aacactcaaa
     9601 tgaaaattag cccatttctg tttatttcag tctgtttgct cacggtatcc tgcacgtata
     9661 aaaaagaggc tgaaactgtc gatactcatc aggtgattta tccggtcgta gaagacgcgg
     9721 aatatagcag caaatatgtc gcccaaattc aatcggtgaa ttatgtagag atacgaagta
     9781 agattagagg ttatattgaa aaaatctatg ttgatgaagg gcaaccggtc aagaaagggc
     9841 agttattgtt tgccttgacc aataacgtat ttgaaaagga attgcaaaaa gcgaacgcgg
     9901 cgtatcgaag tgccttggca gacctgaagg ttgccaaagt tgaactagca aacgtgaccg
     9961 tgttggtaga aaaaaatatt gtgtctaaat cggagctgta tgttattcaa gccaaggtag
    10021 atgcattgaa agccgacgtg gaagaagcga tggccaacaa agagcaggaa gcattaaacc
    10081 tttcgttttc aaaaatacga gctccctttg acggtttcat taaccgtatt ccaaataaag
    10141 taggaagttt gatagccgaa ggagacatct taacctcact gtctgataat cgtgaggtat
    10201 tcgcctattt caacctttca gaagtcgatt atcttaatta cattgcttct ggagaacgag
    10261 ggaccaaggt tgtgagcttt gagctcgcca atcaagtcct ttatgggtat gaaggaacga
    10321 tagaaatgat tgaaagtgaa ttcgacagct caacagggaa tattgcgttc agggccagat
    10381 tccccaaccc cgatggacta ttaaaacagg gggcaaatgg gaaggtcatt gttcataaaa
    10441 tactggaaaa tgcgttgctc attcctcaaa aatcaacatt tgaaatccaa gatcaattgt
    10501 atgtgtacgt ggtgaatcaa gacaatgtgc tggaacaaag gaatattgtt tctaaaatgc
    10561 gactaccaaa tttgtacgtg gtggaatcgg gtttgtctga agacgaacag atcctgttcg
    10621 agggtgtgga gaatgtgatg aatggagaca aggtgcaccc tgttcatgtt gatattgcca
    10681 ccgccatgct gtgaagcagt caagattgag aaacattaga tacctatgac tgcaatattt
    10741 attcatcgtc cggttctctc catcgttata tctcttttca tcacattgct gggcttattg
    10801 tcctttaccc aactccctgt tactcagttt cccgatatta ctcctcccga ggtcaatgtg
    10861 acgtcaaaat ttattggtgc gaatgccgag gcggtggtca aggctgtggt gactccactt
    10921 gagcgagcca ttaatggtgt cgctggcatg gcctacatgg cgtcggtttc tgggaatgat
    10981 ggcaccagcc ttatccagat tatttttaag gccgggaccg acccggagat agccgctgtc
    11041 aacgttcaga acagagtggc ttccgccttg gaagagttac ccccagaggc gattaagtcc
    11101 ggagtgatcg tcgaaaaggt acaaaacagt atgttgttgt atatcaacat actcagtctg
    11161 gatcccacga tagacgaaaa atttctctac aatttcactg atatcaatgt cctgaaggag
    11221 ctgaaaagaa ttgatggcgt cggttttgct gaaattttgg gatcaaggga atatgccatg
    11281 cgtgtgtggc taaggcccaa taaattagtc atgtacaaca tttcagctct cgaggttatc
    11341 gaaaaattac aggcccagaa tgtagaagct gccccgggta aaattggcga aagctcaggt
    11401 aggaataaga acgcccaggc ccttcagtat gtgttgaaat ataccggcaa acacaataca
    11461 aaagaagctt atgaagattt ggtgttaagt agtacggatg atggggaaat tctgcggcta
    11521 aaagacgtcg cagacattga attcgattca caggattatg atgttctctc caaggaaaac
    11581 ggacaagcct ccgctgctat tgtgttaaaa cagcgccccg gaagtaatgc caaggaggtg
    11641 atcaatacga tcaaggagaa aatggaggaa attaaatcga cgtcattccc tccagggatg
    11701 gattacagcc tcagttatga tgtttctcag tttttggatg cctccattca tcaagtcctg
    11761 acaaccctgg ttgaagcctt tcttctggta tccctggtgg tgtttgtctt tttgcagaat
    11821 gttcggctca ccatcatccc tattttggcc gtaccggtat ctctggtcgg cacatttttc
    11881 ttcatgcaca tgatgggttt ttctctgaat ctcattactc tttttgcgtt ggttcttgcc
    11941 ataggtattg tggtcgataa cgcgattgtg gtgattgaag cggttcatgc caagatggag
    12001 ggtttaggaa ttggagccca gcaagctacc gaggaagcca tgcatgaaat tagtggggcc
    12061 attatggcca ttacccttgt catgtcggct gtgtttttgc ccgttgcgtt tatggatgga
    12121 ccgacgggaa ttttttacag gcagttttct ctgactatgg cctgctctat tgttctttca
    12181 gggataaccg cgctcacact gacccccgcg ctttgtgccg tgtttcttaa aaatacccac
    12241 aacagcgagc accctcaacc atcgcgactt cagaaattat ttgatggatt caatcggtgg
    12301 tatgacaatc tttctgataa ctacaaaaaa ctcattagcg cgatagggaa cagaaggatt
    12361 atcacttttg gggttctgct tggtttttcc gtgggcaccg gtttgatcgg ggcgtttgtt
    12421 ccctcgggct ttattcctaa tgaagatcaa ggcacgattt atgccaatgt caccgctcca
    12481 acaggcgcga ctcttgaacg cacggaaaaa gttgtcgatg aagtgcagca aattgcctca
    12541 gaattggagg atgttacttc agtctcaagt cttgccgggt tcagcatcct gtcggaaggt
    12601 acgggtgccg tctacggaat gaatctgatc agtttgaaaa aatggaatga acgaatggct
    12661 tccgataatg aaattatcag ccttcttgaa gaaaaaacca aacatattaa agatgccagc
    12721 attgaatttt ttaccccccc ccctgtgccg ggttatggga attccagcgg ttttgaaatg
    12781 cggttgttag acaaaaccgg ttcgggcacc cttgaggaat tgcaaaaaac ggctgattcc
    12841 tttgtcgatg accttaacaa acggccggaa atcgctaatg cttttaccac cttcaatgca
    12901 cgttttcctc agtttatgct tcatatcgat ggcgagaagg cggctcaaaa gggagtcacg
    12961 gctgaaaatg cgatgaatac ccttcaagca ctgattggca gtgtgtatgc gaccaacttc
    13021 atccgcttcg gccagatgta taaagtcatg gtccaggcgc ttccagagtt tcgtgcagaa
    13081 cctgatgatc tgatgaagtt atatatcaaa aatgacacag gggaaatggt ccacttttct
    13141 tcttttcttc gcgttgaaaa aatttatggt cctgagcagg tgacccgtta caatatgtac
    13201 ccctcagcca tgatcaatgg tcagcctgcc aaagggtaca gcagcgggca ggctattacc
    13261 gccatcaagg aggtggctgc cagtaaattg ccgaaaggtt ttggatacga ttgggcaggg
    13321 tcatcacgag atgaggccca aatgggcaat caggccattt acatttttct gatttgctta
    13381 ttatttgtgt atctactcct cgccgcacaa tatgagagtt ttttactgcc catgccggtc
    13441 attctttccc tacccgttgg gatctttggt gccttatttt tcctgttaat tatgggtttg
    13501 gaaaataata tctacgctca aatcgcgatg atcatgttga ttggcattct cggtaaaaat
    13561 gccattctga tcatcgaatt tgccatattg cagcagaaac ggggaaaaac cgcctttgaa
    13621 gcagcgattg agggggccgc actgcgtttg cgtccaattt tgatgacatc attcgcattt
    13681 attgccgggc tccttccatt gatgtttgcc tcgggagccg gcgagatcgg aaacaatacc
    13741 attggctctg cggcggcggg aggtatgttt ttcgggacca ttttcggcgt gattcttatc
    13801 ccgggcttat atgtggtatt tgcaacgatg ggagagagca aaaatcgaga ggaaagatcg
    13861 aaaacaggga aggcgtcccg gttgagtttt aagtcgaggt ttaaacttgc ccgcaggtta
    13921 gccaaacttc aatagtccgc gtttcgtcag ggaactcagt ccatttttaa cgaattttag
    13981 aattttttct tatacacaat gaaacgtcat atgtattctt ttcttatcat tttgttcctg
    14041 gctctaattt ttaatggctg caccggactg aagaccgatc tggccattca ggaaaacgaa
    14101 ataccaacaa gctttaagga caaaacagat accacgaata tcgctgacat ccaatggaaa
    14161 gattactttg ttgatccgct cctccatgaa ttaattgaga ctgccataga gaataacctt
    14221 gatttgcaaa tggctttgca gcgaattgaa acttcgcgtt caagtgtccg ggccgccacg
    14281 ggggcactga tgccgacaat gggtttgggt gtaggaaccg ccattcgccg atacggactg
    14341 tacaccatgg acggggcagg gaacgcgacg accgacttta aatcaggtca acaaattcct
    14401 gataacttgc cggatctttt tgtgggctta cagtcatcgt gggaagttga tatttggggg
    14461 aagttgcaaa accagcgcag ttccgcaatg gcgaaatttc tggccagtat cgaagggacg
    14521 agctttgtcg tttctaatct cgtagcggat gtagccatct attataacga attgcaggct
    14581 ttggataatg agttggacat catcaggcaa accctccaca cacagtacga atcgttggag
    14641 gtggttgaac tgcagaagga agccggcagg gcaactgaat tagccgtgga actattcaag
    14701 gctcaagtgc tcaataccag tattttggaa aaagaggtcc tgcaacaaat tacgatcacc
    14761 gaaaatcaca taaacttttt gttagggcgc tatccccaac ccattgaacg gaccaaaggc
    14821 caatatttct cggaaattcc acatgagatt tcatcgggtg ttccttcaca attattggca
    14881 aaccgcccgg atgttcgtga agccgaattg cagatcaagg gcagtaattt cgatttgaaa
    14941 gtagctaagg ctgcattttt cccaagcttt aacatcacgg caagttttgg gtttcaggca
    15001 ttcaatcccg attttttatt tgtggcccct gcctcaatcg cctataccgc cgtaggcacg
    15061 ttggtcgcac cacttattaa tagaaacgcg ttacaagcac agttcaataa tgccgaagcc
    15121 aatcagttga ctgccatgta tcactaccaa aaaacaattt tgaacgccta tgtggaagtg
    15181 gccaatgagc tctctaatat tcataacttg cgaaaaatca atattctgaa aaagaaacaa
    15241 aacgaagtgt tgcgacaatc cgttgaaaca tcacacttac tctatagata tggccgggca
    15301 acctacctgg aggtcctcac tgcacaaagg aataccttgc aatccgatct cgaattgatc
    15361 aattttgcca agcaacttcg tatcgcggca gtaaaaattt acaaggcatt aggcggtgga
    15421 tggaaataaa atccctattt attggaatca ttcttggatt gagttgaaaa cacggtaggg
    15481 gatggatatt tttaat
//
LOCUS       DAORAQ010000058        15447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_651_length_15447_cov_64.550741, whole genome shotgun sequence.
ACCESSION   DAORAQ010000058 DAORAQ010000000
VERSION     DAORAQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15447
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_651_length_15447_cov_64.550741"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..96
                     /locus_tag="PKM72_07165"
     tRNA            9..96
                     /locus_tag="PKM72_07165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Ser,seq:tga)
     gene            271..2034
                     /locus_tag="PKM72_07170"
     CDS             271..2034
                     /locus_tag="PKM72_07170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNP60604.1"
                     /translation="MSKTLPSWDLQGLLRHPTKDFKRITKKLDSLISELEAVRPQLSP
                     DISAARFKKIWEQYETVTEHMTTLRAFSSLWFSENTKNQEARAFDTQVRNRLTDFSNR
                     LVFLDLWWQSLDPTNAARLTEKAERFRYHLDTLTRFTPHTLSESEERILTIKSTTGRQ
                     ALETLYGVTTNSLTFPMKIKGRTTRLTREQLMGYVRHPLASVRREAYHALFKVYGEHR
                     DALGEMYKSLVQDWGNEGLTLRHYASPMAVRNVTNDVPEKAVDALLKTCRKNAVIFQE
                     YFRLKGRLLKMKQFQRYDLYAPFAAKKSKYSFARAKELVMEAYQAFSPELAQRAKQVL
                     EENHLDAAIRPGKMGGAFCYSVLPTQTPYVLVNFTGEPRDVSTLAHELGHAVHAMMAK
                     EHSIFTFHSSLPLAETASVFGEHLLSDLLLQQEKDPKVKAGLLVDQLDDAYATIMRQA
                     YFVQFERQAHQMVQQGATIDALAQTYLALLREQFGPRLPVDQEFQWEWLAIPHIFGSP
                     FYCYAYSFGSLLVLSLFQRYQTEGSSFVPRYLQLLSGGGSASPQDLLKPLQVDINSTT
                     FWQAGFTRIQGLVNELERSMP"
     gene            complement(2144..2572)
                     /locus_tag="PKM72_07175"
     CDS             complement(2144..2572)
                     /locus_tag="PKM72_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP60605.1"
                     /translation="MNHHSKGTILRGFAGLLCWFVIGCSENGTSAGESSAPASIAQIS
                     VVKDLPTEFKEGEEKFNAFCSPCHGSQASGTGQGPPLVHKIYEPSHHADFAFQRAAAQ
                     GVKAHHWKFGNMPKIEGVTADDVTQIIGYIRWLQRQAGIS"
     gene            complement(2631..3317)
                     /gene="queC"
                     /locus_tag="PKM72_07180"
     CDS             complement(2631..3317)
                     /gene="queC"
                     /locus_tag="PKM72_07180"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNP60606.1"
                     /translation="MPKSRIVVLASGGLDSTVTAAIAKSEGHELYFLTIDYGQRHRTE
                     IDCAHKISEYFEVKEHKIVQLDLRTFGGSALTDQIPVPAHQPARDRERQIPITYVPAR
                     NTIFLSLALAYAEVLGATSIFFGANIRDYSGYPDCRPAFIEAFKEVARLGTQMGLEGR
                     QIDILAPLLFLTKREIIQKGHALGVPFQWTNSCYNPGRHNQPCGQCDSCVIRQEGFQE
                     AQLPDPLLAG"
     gene            complement(3511..4014)
                     /locus_tag="PKM72_07185"
     CDS             complement(3511..4014)
                     /locus_tag="PKM72_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNP60607.1"
                     /translation="MAERPVKKETKATISAGTKKTTAKATSTTPAPRKPAKAQTEDRH
                     VALKEILLAKREALIQEIKQQLGQSVTEEQQRRLEAAMDSGDQALVDLDREMGISLQE
                     MRNRERQLIDDALDSLDEGTYGVCAECGGEINEKRLQALPFARLCVECKSKRELMEKI
                     ERSEQRQ"
     gene            complement(4127..4642)
                     /locus_tag="PKM72_07190"
     CDS             complement(4127..4642)
                     /locus_tag="PKM72_07190"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNP60608.1"
                     /translation="MDYKAHIREVPDFPKPGILFYDITPLLNNPVCFRSIIDECIRHY
                     QDAGITRVVGIESRGFILGSPLAYHLNAGFVPVRKPGKLPADVFEVNYNLEYGSTTLS
                     IHRDAIQEGERVLVVDDLLATGGTAGATVHLIRQLGGDIAGVVFLVELLGLEGRNKLN
                     GCEIHSLITYE"
     gene            complement(4728..5087)
                     /locus_tag="PKM72_07195"
     CDS             complement(4728..5087)
                     /locus_tag="PKM72_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNP60609.1"
                     /translation="MLLTIQQRNLDARYPVEEELKEQMEKIVRAHACVSGTVQGVGFR
                     AFVQMQANRRSLHGWVRNRAGGEVELEVEGPHASVQTFLQTLHKGPPLSQVLQVIVDW
                     KDPNRQTEGFQILRTFL"
     gene            complement(5038..6519)
                     /gene="hemG"
                     /locus_tag="PKM72_07200"
     CDS             complement(5038..6519)
                     /gene="hemG"
                     /locus_tag="PKM72_07200"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNP60610.1"
                     /translation="MRKTPYQVVIVGGGISGLATSYAIMEEAEKTQTAVQCKVVERDP
                     RWGGKILTHVTDKYLIEGGPDSFLTSKPWALELCRTLGLQDQLISTNSQHNQTFSFCR
                     GALRELPQGLLAFRPRRVDTLVSSGLLSWGGMLRMAAERFWPRRNPWPADESMGEFFR
                     RRFGTEAFENLIEPLVAGIYAGDADELSIESTFPRFRELEREHGSVIKGMRKALGAAP
                     AAPRSSGDTPTMFMSLRGGLGELIRTLVEALRRRGVELIGGAECQEIQSPAPESGVFH
                     VMLDNGDRLPADAVVLATPAYQTAQLLRAIQPEAASLLDGIPYASTATISMAYPPESI
                     RSQIRGFGFVVPRKEQRPLLAATWTSLKWPDRSRAGETLIRCYIGGRGRESVLEQDDD
                     SLVECVRGELTSLVGITTAPTYTEVHRWKQGMPQYVLGHRDRLAKLQEQLAHSPGLYV
                     TGAGMYGIGIPDCIREATRVGKLLLQDYAAHHSAKKSGRTVSG"
     gene            complement(6621..7652)
                     /gene="hemE"
                     /locus_tag="PKM72_07205"
     CDS             complement(6621..7652)
                     /gene="hemE"
                     /locus_tag="PKM72_07205"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNP60611.1"
                     /translation="MNDRFIKACMRQPVDRTPVWFMRQAGRYMEEYQAIRRKYSILEV
                     CKTPALAAEVTLQPINRFDLDAAIIFADILLPLQAMGLNLEFVEGKGPVIDNPIRGEM
                     GIVDLRQVDGEAFAYAGEAIRQTLQALDNRVPLLGFAGAPFTLASYAIEGGSSRDYAR
                     TKHLMFSQPTVWHQLMSKLSVVVVEYLRVQSRAGAQAIQLFDSWVGSLSPGDYEEYVL
                     PHVQNIFSALRVEGIPLIYFGTGTTGLLPLMRKAGSDVMGVDWRVRLDEAWRTIGFDV
                     SIQGNLDPLVLFAPEKEIERRVKDIMQQAGGRPGHIFNLGHGILPNTPLHSVEFAIEC
                     VRRFSTASS"
     gene            complement(7810..8244)
                     /locus_tag="PKM72_07210"
     CDS             complement(7810..8244)
                     /locus_tag="PKM72_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60612.1"
                     /translation="MAETFANARWVKEGFLDNRIVGSVVGQITLAGLETVDVYLVGDF
                     VGEIAGKIVGFQNPRFLDDSQAGGSLYDFSIPQLGKVSLMSFDPHPLLDPHPYFEWFS
                     TDQQHYRIELQPDEAWVMSGDQRDVVEGQAQRIRQELAPLLP"
     gene            complement(8322..8525)
                     /locus_tag="PKM72_07215"
     CDS             complement(8322..8525)
                     /locus_tag="PKM72_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60613.1"
                     /translation="MGYWIHLKRSLDEAMIHRDRCPEVPSGPHRADFWEGGWTEYPDK
                     DTALENLENTDATLQLKCPLCKP"
     gene            complement(8645..8824)
                     /locus_tag="PKM72_07220"
     CDS             complement(8645..8824)
                     /locus_tag="PKM72_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60614.1"
                     /translation="MSVLIRKFKLPTGMVKEERIDDPDRIERYMQFFDKEQQAKLDAG
                     QKVFLDKDEWQFVDN"
     gene            9027..9320
                     /locus_tag="PKM72_07225"
     CDS             9027..9320
                     /locus_tag="PKM72_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNP60615.1"
                     /translation="MPLYEYVCEGCDHRYEAMQALSVKPEETLCPKCNTTKSRRIMSS
                     FASKIVGTHKPGFAEGKAYNMLNERMDKFSKLPPIMGQRAEPTESNFNPPAGE"
     gene            9496..10404
                     /locus_tag="PKM72_07230"
     CDS             9496..10404
                     /locus_tag="PKM72_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNP60616.1"
                     /translation="MIKIMAAIRLGKYNAVKITFLIGIVFLCGLIFPLGPVHSKESTK
                     SEPPVTDSGTISGTILIVGNGPERYLLEILAAGFESRHPSVSVDIFWHPNAKPIRTIE
                     LNEADIGITGEEVPSLRSTVIARDGIAVLTNFSNPVKEMTIQQVADVFSGKIRYWSQV
                     HEEAPQTKIVLINRTPNENIRQGFEKTLHIPDGIPRSAHRAGTENEAIKAVSGNLEAI
                     TFVSMTPALRAKEDGIAINLLFINRVEPEVQTILDNRYPLQRPVMLITQSSPSQQVLA
                     FEQFALSPEGQNLMRKGKYYPLLKDQ"
     gene            10522..11940
                     /locus_tag="PKM72_07235"
     CDS             10522..11940
                     /locus_tag="PKM72_07235"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNP60617.1"
                     /translation="MLKKVLVANRGEIAMRVIRACREFGIATAAIYSESDATAIYVKK
                     ANEAYLVGPGPVQGYLDARQIVGLAKRIRADAIHPGYGFLAENADFARLCEEAGILFI
                     GPSPHSLELLGDKVQARALAIKIGVPVVPGTDGSVGTLEEALTFSHKAGYPVMVKASA
                     GGGGRGLRVVRSDDELQEAMEAGAREALAAFGNGDLFLEKYVERPHHIEFQLLADKNG
                     YVIHLGERDCSIQRRHQKLIEIAPSLVLTPRLRAEMGEAAIAIARAAQFYNAGTVEFL
                     LDPDGRYYFMEMNPRIQVEHTVTEQITTVDIVQSQLWIAAGRPLVFGQHEVNLQGYAI
                     QCRVNAEDPQNGFRPSSGKITAYLSPGGVGVRIDGAVYKDYTVPPYYDALLAKLTVHG
                     RTWDETVRRTHRSLEEFVLRGVKTTIPFMAKLMEEPDFRAGRFDTSYLERHPELFEYE
                     EAEEPEDLVIALSAAIAAFEGL"
     gene            11990..>12658
                     /locus_tag="PKM72_07240"
     CDS             11990..>12658
                     /locus_tag="PKM72_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60618.1"
                     /translation="MSRKTSHTHKSKKPSRPVPTRMKLDAAPALDIEASPKRQVFLTD
                     VALRDGHQSLLATRMRTEDLLSVASELDEVGFWSLEVWGGATFDSCLRFLKEDPWERL
                     RAFREAMPKTRLQMLLRGQNLVGYRHYADDVLEKFIELSAQNGIDVFRIFDALNDIRN
                     IKPAMEVVKACGKHIEATICYTVSPVHSLNHFVEQAKQLEDLGADTICIKDMAGLLPP
                     FEAYE"
     assembly_gap    12659..12758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12759..13802
                     /locus_tag="PKM72_07245"
     CDS             <12759..13802
                     /locus_tag="PKM72_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNP60619.1"
                     /translation="APAADKLRKARKRYRQFESDFTGVDTDILLSQVPGGMLSNLAAQ
                     LAEQNALGKIKEVLEEIPRVRKDMGYPPLVTPTSQIVGTQATLNILTGERYKVITNET
                     KNYFQGLYGKPPGTLNSKIRQKALEGDVPVAGRPADNLDPELGDATQELVGREMAEED
                     IVSYALLPSVALQFFDERERGELKPEPLHASPESGPAVAHDLHLAPVEFNVTVHGEAY
                     HIRVSGSGQTVDGVKPYYIKVNDKLEEVYLEPIQEVLAGSPDPPPSQASKTEKRPKPS
                     QPGDVTTPMPGRVVKVLVTEGSTVNVGDSLLVVEAMKMENRVQSPIAGSVITIYVKEG
                     DEVNPDETLIHLE"
     gene            13805..14758
                     /locus_tag="PKM72_07250"
     CDS             13805..14758
                     /locus_tag="PKM72_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNP60620.1"
                     /translation="MRGCSHFRLFLLFMVVLGCLTILGCSSLKTLPSGIDLIQRVPYQ
                     TTRVNTHRIAYLDVGQGPPLILIHGFGGSMWQWEHQYSVLAQTHRVIILDLLGSGLSD
                     KPDDAYTPKYLVEFFRQFMDALNISRATLVGNSMGAGLAMAMALDYPERVDRLVLISG
                     FPAHVENSIASPHYQRFLHHRPPIWLAKLGNWMAGRRTTEYILKEIIYNAALISPAII
                     DRSFHNRQRGDFLVPLYSLMDNIKTWEGQYGNRFQQISHPVLLLWGDQDRVFPLDVGE
                     RVKDQLPHVEWHVIPEAGHLAQWEAPTMVNQFILSFLERDF"
     gene            14873..>15447
                     /locus_tag="PKM72_07255"
     CDS             14873..>15447
                     /locus_tag="PKM72_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNP60621.1"
                     /translation="MQKNSSIMSLLFILAASSLAGCSTLNGQSDQNFSYKNIPIDKET
                     ARTGEGSTILFRGTPLPLSGMEVKTGETLRAVPLAKGDLSLVNIHESQGKVRIISIVP
                     SLDTKVCEQQTHYLSEKNKGLDQQVSLITISVDTPFAQERFAKEAGIANVEFLSDFRG
                     GEFGISHGLLLERPHVLARAVMVVDANNVIR"
BASE COUNT         4031 a         3949 c         3531 g         3836 t
ORIGIN      
        1 aataattcgg aggggtgcta gagtggtcga atagaccggt cttgaaaacc ggcgtggtga
       61 aagccaccgt gggttcgaat cccaccccct ccgccagcct tcgccaaggc ttcggctggc
      121 aagccagcac agaagagagc caaaatttca ccaagtaaac taccgaagta aggagggcga
      181 aggctgtccg ccgaagcttt agcgaaggag gacacaacca ctcttcaaaa tagaatatac
      241 tgttcactat atccccatta aggagcacat atgtcgaaga ctcttccatc ctgggacctt
      301 caaggtctct tgcgccatcc gaccaaagac ttcaagcgga tcaccaaaaa gcttgactcc
      361 ctgatttctg aactcgaggc cgtacgaccg cagttatccc cagatatctc cgccgcccgg
      421 ttcaagaaaa tctgggagca atacgaaacc gtcaccgagc acatgacgac tttgcgggct
      481 ttttcgtctt tatggttttc agaaaatacg aaaaatcagg aagcccgggc gtttgacacc
      541 caggtacgaa acagactgac agatttttca aatcgactgg tctttcttga cctctggtgg
      601 caaagcctcg atcccaccaa cgcggcccgg ctgaccgaaa aagccgagcg gtttcgatat
      661 catcttgaca ccttgactcg attcacaccg cataccttga gtgagtctga ggaacgaatt
      721 ttaaccatta aaagtacgac cggtcgccag gctctcgaaa cattgtatgg cgtcaccacg
      781 aatagtttga catttcccat gaaaataaaa ggccggacga cccgtctgac ccgcgaacaa
      841 ctcatgggat atgtccggca tccattagct tcggttcgtc gagaagccta ccatgcccta
      901 tttaaggtct acggtgaaca ccgtgatgct cttggagaaa tgtataaaag cctggttcag
      961 gattggggta atgaaggtct caccttgcgg cattacgcct cgcctatggc cgtgcgcaat
     1021 gtcacaaatg atgtccccga aaaagccgtt gatgctctgc tcaaaacctg tcgaaagaat
     1081 gccgtcattt tccaggaata ttttcggtta aagggacggc tgctgaagat gaaacaattt
     1141 caacggtacg atttgtatgc tcccttcgcg gcgaagaaat caaaatattc atttgccagg
     1201 gcgaaagaac ttgtgatgga agcctatcaa gccttttccc ctgaattggc tcaacgggcg
     1261 aagcaggttc ttgaagaaaa ccatcttgat gctgcgattc gaccagggaa aatgggtggg
     1321 gccttttgct atagcgtcct cccgacacag actccctacg tactggtgaa ttttacggga
     1381 gagccacgtg atgtctcgac gttagcccat gaactgggac atgccgttca tgccatgatg
     1441 gcaaaggagc attccatttt cacgtttcat tcgtcgctcc ctctggcgga aacggcctct
     1501 gtgtttggcg agcacctgct ttcggattta cttctccaac aggaaaagga ccctaaggtc
     1561 aaggcgggac ttcttgtcga tcaactcgac gatgcgtatg ccaccattat gcgacaggcc
     1621 tattttgttc aatttgaacg gcaagcacat cagatggttc aacaaggtgc gaccattgat
     1681 gctcttgctc aaacctacct ggcactcctt cgtgagcaat tcgggcctcg cttacccgtg
     1741 gaccaggagt ttcaatggga gtggctagcc attcctcata tttttggcag tccgttttat
     1801 tgctacgcct acagctttgg cagtttatta gtactgtcgc tttttcaacg ctatcaaaca
     1861 gaaggatcct ccttcgtccc tcgatattta caattgcttt caggaggtgg gtcggccagc
     1921 ccacaagact tgctcaaacc cctacaggtg gatatcaact caacgacttt ctggcaagcg
     1981 ggctttacaa gaatccaagg actggtcaat gaactggaac gaagtatgcc gtgaaggatc
     2041 tggcttcaaa cggtttcaac tcgtgggagc aaacaacagt cagtgttccc acgatggcca
     2101 tggtgacaga agaaagccga actggaccac gccctgggat gccttaggaa attcctgctt
     2161 ggcgttgaag ccacctgatg tagccaataa tctgcgtgac atcatcagcg gtcacgcctt
     2221 cgatcttcgg catgttcccg aacttccaat ggtgggcttt gactccctgg gccgcggccc
     2281 gctgaaaagc aaaatccgca tgatgactgg gttcataaat cttatgaact aatggaggac
     2341 cttgacccgt tcccgaggct tgggaaccat ggcaaggaga acaaaaggca ttaaattttt
     2401 cctcgccttc tttgaattct gttggaagat cttttactac cgatatttgt gcgatggatg
     2461 cgggagcaga agattccccg gcactcgtcc cattctcaga acagccaata acaaaccaac
     2521 aaagaagccc tgcaaaaccc cggagaattg tccctttaga atgatggttc atgtccaaat
     2581 tcctttacac ccatactcgg tcacaatcag acgtggccac agcctcatat ttatccggcc
     2641 agtagcggat cgggaagttg ggcctcttga aatccctctt gacgaattac gcaactatca
     2701 cattgtccgc acggttgatt atgtcgtcct ggattataac aactattcgt ccattgaaaa
     2761 ggcaccccta aggcatgtcc tttttggata atttcacgtt tggtcaaaaa gagcagcgga
     2821 gcgagaatgt cgatctgtcg accttccaat cccatttgtg tgccgagacg ggccacctcc
     2881 ttgaatgcct caataaatgc cggccgacaa tccggatagc cggaatagtc acgaatgttg
     2941 gccccaaaaa aaatactggt ggcacccaac acctcggcat aggccagcgc caaacttaag
     3001 aaaatggtat ttctggccgg aacgtaggtg atgggaatct gccgttcacg atcccgtgcg
     3061 ggctgatggg cgggaaccgg tatctgatcg gttaaggccg atcccccaaa cgttcgtaag
     3121 tccagttgga ctattttgtg ttctttgacc tcaaaatatt cagaaatttt atgcgcacaa
     3181 tcaatttccg ttcgatgacg ctgtccataa tcaatagtca ggaaatacag ctcatgtcct
     3241 tctgatttgg caatggcggc cgtcaccgtg gaatccaacc cgccacttgc tagaacaaca
     3301 attcttgact taggcatacg agaatgttcc ctgaaccctt ttcctctcgc ctatcagatg
     3361 gctagcagag gggtataagg aaaaatcagt cacgggctca ggttttgttg gaaagaaagt
     3421 tgaaggcgca aaacgctttt gtcgaattgg atgagtgtat gccactccca attagacagg
     3481 gtgagagagc agatgcagca atccttctga ttattgccgc tgttccgatc gttcaatttt
     3541 ttccatcagt tcccgtttgg atttacactc gacacacaaa cgcgcaaacg gaagggcttg
     3601 caaacgcttt tcattgatct cgcccccaca ttctgcgcac acaccatagg tgccctcatc
     3661 caaactatcc aaggcatcat caatcagttg tcgttcgcga tttcgcattt cttgaagcga
     3721 aatccccatt tcgcgatcca aatccaccag cgcctggtcc ccgctgtcca tcgccgcttc
     3781 cagccggcgt tgttgttctt ctgtcactga ctgaccaagc tgttgtttga tttcttgaat
     3841 cagcgcttcc cgtttggcca agagaatttc ctttaacgcc acatgacgat cctctgtttg
     3901 agctttcgcc ggcttccgcg gagcaggggt tgtggaagtg gcttttgctg tggttttttt
     3961 cgtcccggcg gagatcgtcg ctttcgtttc tttctttact ggtctttcgg ccatactaca
     4021 ttcctttctt ccttcgaaag ttaaccattc tctcacacga ctggaggatc aatgtctgcg
     4081 gacaccttcc ccgttgcatc atgcaatacg agtaaagtct gtgtccttat tcatacgtaa
     4141 tcagggagtg aatttcacac ccgtttaatt tgtttcgtcc ttcaagccca agtaattcca
     4201 ctaaaaacac cacgccggca atatctccac ccagttgccg aatgagatgg actgtcgccc
     4261 cggccgttcc tccagtcgca aggagatcgt caaccacaag cactcgctct ccctcttgaa
     4321 tcgcatcacg atgaatcgac aacgtcgtcg agccatactc taaattataa ttgacctcaa
     4381 acacatcggc cggtaatttg ccaggcttcc ggacagggac aaatccggca ttcaagtgat
     4441 acgccaatgg gcttcctaaa ataaaccctc gtgattcaat cccaaccacc ctggtaatac
     4501 cggcatcctg gtagtgtcga atgcattcat caataatgga acgaaagcaa acaggattat
     4561 ttaataaggg cgtaatatca taaaagagga tacccggctt aggaaaatca ggaacctctc
     4621 gaatatgcgc tttatagtcc atagataaac tacttaatct tgataagacc tgtggtttct
     4681 tccaactttt agatcggaat tatccgatcc gcaccgccct ctatccctta cagaaaagta
     4741 cgaaggattt ggaacccttc agtttgcctg tttgggtctt tccagtcaac tattacctga
     4801 agaacttgag agagaggagg ccctttgtgg agagtttgga gaaaggtctg aacagaggcg
     4861 tgtggcccct caacttccaa ttcgacttct cctccagcgc ggtttcgcac ccacccatgc
     4921 aatgacctcc tgttggcctg catttgtaca aatgctcgaa atccgactcc ttgaactgtt
     4981 ccactcacac aggcatgagc ccgaacaatc ttttccatct gctctttcag ttcctcctca
     5041 accggatacc gtgcgtccag atttctttgc tgaatggtga gcagcataat cctgtagtag
     5101 cagttttcca accctcgtgg cttctcggat acaatcaggg ataccgatgc catacattcc
     5161 tgctcctgtc acatagagac ctggcgaatg agctaactgt tcctgaagct tcgccaaccg
     5221 gtcccgatgt ccgagcacat attgaggcat tccctgcttc catcgatgga cttctgtata
     5281 ggtcggagcg gtcgtgatcc cgaccaaaga cgttaattct ccccgaacac actccacaag
     5341 tgagtcatca tcctgctcaa gaaccgactc tcgccctcga ccaccgatgt aacaacgaat
     5401 cagagtttct ccagccctgc tcctatccgg ccactttaac gaagtccagg ttgccgcaag
     5461 caatgggcgt tgttcttttc gaggaacaac aaatccgaat cctcgaatct gcgacctaat
     5521 ggattcaggg ggataggcca tagaaatcgt agccgtcgag gcatagggaa tgccatctaa
     5581 cagactcgct gcttcaggct gaatggcacg aagcaactga gcggtctgat aggccggagt
     5641 ggccaacaca acggcatcag ctggaagccg atctccatta tccaacataa cgtgaaatac
     5701 gccagactca ggagccggag actgaatttc ctgacactcg gcacctccaa ttaattctac
     5761 tccacgtctg cgcaatgcct ccactaaggt tcgaatgagc tcccctaatc cccctcgaag
     5821 cgacataaac atcgtgggag tatcaccgga ggaacgcggg gcggctggcg cggcgcccaa
     5881 agccttccgc attcctttaa tgacactgcc atgttctcgc tctaattcgc gaaatcgagg
     5941 aaaggtcgac tcaatactca gttcatctgc atccccggca tagatgcctg ccaccaatgg
     6001 ttcaatgagg ttttcgaagg cttcagtccc aaaccgtcga cgaaaaaact cgcccattga
     6061 ttcgtctgcc ggccaaggat tccgccttgg ccaaaagcgc tcggccgcca tccgaagcat
     6121 tccaccccag gacaagagtc cgctagaaac aagggtatca acccgtcgtg gccgaaaggc
     6181 caataatcct tgtggaagtt ctctcaaggc accccggcaa aatgaaaacg tttgattgtg
     6241 ttgtgagttg gtagaaatta attgatcttg aagccccagg gtccggcaca attcgagcgc
     6301 ccacggcttt gaagtgagaa aggagtcagg tcccccttca atcagatact tgtctgtgac
     6361 atgtgtcaga attttcccac cccatctggg atctcgttca acgactttgc attgtacggc
     6421 cgtctgggtt ttctcagctt cttccatgat ggcatacgac gtggcgaggc cggaaatccc
     6481 tcctcccaca ataaccactt ggtacggtgt cttcctcatg aagtctcgag caatcaacga
     6541 ggaaatgcta gaaagtagtg agaaattgaa ataaacaggt tttcatggag ggatggggaa
     6601 aagctgacag gaaaaagcac ctacgaggaa gccgtggaaa accggcgaac acattcaatg
     6661 gcaaattcaa cgctgtgcaa aggggtatta ggaagaattc cgtgacctaa attaaatatg
     6721 tgaccgggtc ggccgccagc ttgctgcata atatccttca cccgacgctc gatttctttt
     6781 tcaggggcaa acagtaccag ggggtccaga ttaccttgaa tactcacatc aaacccaatg
     6841 gtcctccatg cttcatccaa acgaacacgc cagtcgacac ccatgacatc gcttccagcc
     6901 tttcgcataa gtggaagaag cccagtcgtc cctgttccaa aatagataag gggaatccct
     6961 tcaacgcgta gggctgaaaa aatattttga acatgaggaa ggacatattc ttcataatcg
     7021 ccaggactta agcttccgac ccaactatca aaaagctgaa tagcttgggc gcctgctcga
     7081 ctttgcaccc gtagatattc cacgacaacc acagatagtt tggacattaa ttgatgccac
     7141 acggttggct gcgagaacat caaatgtttg gttcgggcat agtcacgtga gcttccgccc
     7201 tcaatggcgt aactggctaa cgtgaacggc gccccggcaa atccaagtaa ggggactcga
     7261 ttatcgaggg cctgaagtgt ctggcgaatg gcttccccgg cataagcgaa ggcctcccca
     7321 tcgacctgac gcaggtccac aatgcccatc tcacctcgta tcggattatc aataaccgga
     7381 cccttcccct caacaaattc caggtttaat cccatggctt ggaggggcaa taaaatatcc
     7441 gcaaaaatta ttgccgcatc cagatcaaag cgattaatcg gttgaagggt gacttcggca
     7501 gctaacgccg gtgttttgca tacttctaaa atcgaatact tgcgtcggat agcctggtac
     7561 tcttccatgt accgccctgc ctgacgcata aaccaaaccg gggtccggtc tacaggctgt
     7621 cgcatacagg cttttataaa tcggtcattc atggctggca ttctacgaag ggagaaaaag
     7681 cgagtcaatc aataacgttc acaaagtgat gctggttatt attatgttcg gcagattgtc
     7741 tttctaatca aaaatgatct ccctctctta cctgttaaat acgacgggat accaccaatc
     7801 catttccgtt catggtaaaa gaggtgccag ctcttggcga attctctggg cctgaccctc
     7861 cacaacatct cgttgatcac cagacatgac ccacgcctca tccggttgca gttcaatgcg
     7921 ataatgttgt tgatcagtcg aaaaccattc aaagtaagga tgggggtcaa gaagagggtg
     7981 cggatcaaac gacatgagac tgactttgcc tagttgaggg atggaaaaat catatagcga
     8041 tcctccggct tgagaatcat ctaaaaatcg tgggttttga aaacccacga tttttccggc
     8101 aatctctcca acaaaatctc caaccaaata cacatccacg gtttccagtc cagctaaggt
     8161 gatttgccca acgaccgatc cgacaatccg attgtctaaa aaaccttcct tcacccaccg
     8221 ggcgttagca aatgtttccg ccataacaag tactatttaa aaaagaaaaa tccctctcac
     8281 ggactggttg tactgaaagt cacaaaatca gaacccacga gctacggttt acataaagga
     8341 catttgagtt ggagggtggc atccgtattt tccaaatttt ccagagccgt atccttatct
     8401 ggatattcgg tccatccgcc ttcccaaaag tccgcccgat gcgggcctga aggcacctcc
     8461 ggacaacgat cccgatggat catcgcttca tcaagtgatc gtttcagatg gatccaatac
     8521 cccatagtcg tgatgctcct tcactggatg gtacgccgag cctgccccac aggcacgatc
     8581 aaagatttac gtgaaaacaa cctgtcagaa atattaacga agagggagcc tgacctgcag
     8641 gaattcagtt atcgacaaat tgccattcat ctttatctaa aaaaactttt tgccccgcat
     8701 caagcttggc ttgctgttcc ttatcaaaaa actgcatata ccgctcaatg cgatccggat
     8761 cgtcaatacg ttcctccttg accattccag tgggcaattt aaatttccta attagcaccg
     8821 acatgactca ttcctccaaa atttgactgt aaaaagacca acgaaaaatt atagtgaaat
     8881 cataaacttg ccgtcaatat ttttttggac atttccatag tcacttcggt tatccattgg
     8941 ccacgcctag gcattcttcg atatactggg gtcctgacag actcgatatt aatcatgttc
     9001 atcgtataat ttcaaaggag tccggtatgc cattgtatga atatgtctgc gaaggttgcg
     9061 atcatcgtta tgaggccatg caagcgctta gcgtgaaacc agaagaaact ctttgcccta
     9121 aatgcaacac caccaaaagc cgacgcatca tgtcctcgtt tgcgtcgaaa attgtgggaa
     9181 cacataaacc aggatttgca gaaggcaaag cctataacat gttgaatgaa cgaatggata
     9241 agttttccaa actccctcct attatgggcc aacgcgcaga gccgaccgag tcaaatttta
     9301 accccccagc aggagaatag gatgaggctt gggcggacat gtataaagct gaccgctcaa
     9361 ggtattctat cttccacaat tcccgcccct tgacatttat ttctataaac cggaaagatg
     9421 tgcccgtttt tttattcttg gtcatatcgt aacctgtttt ttcgaccact ggcgtggccg
     9481 ttgtttttgg tagggatgat aaaaattatg gcggccatca ggcttggtaa atataatgcg
     9541 gtcaagataa cttttctgat cggaatcgtt tttctctgtg gactgatatt tcccttaggc
     9601 ccggttcatt ccaaggaatc tactaaatcg gaacctccgg ttacggattc agggacgatt
     9661 tccggaacga ttctcattgt tggcaatgga cctgagcgat atcttctgga aattctggct
     9721 gcgggatttg agtcccgcca tccttctgtc tccgttgata ttttctggca tcctaatgcc
     9781 aaaccaatca gaaccatcga acttaatgaa gctgatattg ggatcacggg cgaggaggtc
     9841 ccttcgctcc gatccacggt cattgccaga gacggcattg ccgtcttaac caatttttca
     9901 aacccggtaa aagaaatgac tatccagcaa gttgcggatg tcttttccgg gaaaattcgt
     9961 tattggtctc aggtccatga ggaagcccct caaaccaaaa ttgtgctaat caatcgcacc
    10021 cctaatgaaa atattcgaca aggtttcgaa aaaacattac atattccaga tgggattccg
    10081 cgttccgctc atagggcggg aaccgagaat gaagccatta aagcagtgag cggaaattta
    10141 gaggccatta cctttgtatc catgacccca gctctccggg ccaaagaaga tgggattgcg
    10201 attaatcttc tgtttatcaa tcgggtggag cctgaagtgc aaaccatatt ggacaatcgt
    10261 tatccactcc aacgaccggt gatgctcatc actcaatcca gtccctcaca gcaggttctg
    10321 gcctttgaac agtttgccct ttccccagaa ggccaaaacc taatgcgaaa agggaaatat
    10381 tatccacttc tgaaggacca ataattcgcg gaatccctga ttcgttagct tatcttctga
    10441 taccatctgg cctcgaaaaa ttcagaacac gatcggcacg gtcatgcatt cctaaagttg
    10501 agacacgaac actagaatcg tatgttaaaa aaagttttgg ttgccaatcg gggagaaatt
    10561 gcgatgcgcg tcatcagggc atgtcgagaa ttcggcattg ccacggcggc gatttattca
    10621 gaaagtgacg ccaccgccat ttacgtcaaa aaagcgaatg aagcatattt agtcggtccg
    10681 ggtcctgtgc aaggctatct tgatgctcga cagattgtag gattagcgaa gcgtattcga
    10741 gccgatgcca ttcatccagg gtatggattt ttggctgaaa acgctgattt tgccaggctt
    10801 tgtgaagagg caggcattct ctttattggc ccatctccgc actcacttga actactcggc
    10861 gataaggtcc aggctcgagc actggccatt aaaatcggtg ttcctgttgt tcccgggact
    10921 gacggcagtg ttggcacgtt ggaagaggca ctgacatttt cccataaggc gggatacccg
    10981 gtcatggtca aagccagcgc cgggggtgga gggcgaggcc ttcgcgtcgt gcgatcagac
    11041 gatgaattac aagaggcaat ggaagctggg gctcgagaag ccctcgccgc ttttggcaat
    11101 ggtgatttat ttcttgaaaa atatgtggaa cgtccacacc atattgaatt tcaattgctg
    11161 gcagacaaaa acggctatgt gattcatttg ggagaacgtg attgttctat ccaacggcgc
    11221 catcaaaaat tgatagaaat tgccccgtca cttgtattaa cccctcgtct aagagcagaa
    11281 atgggagaag ccgccatcgc catcgctcgc gccgcccaat tctataatgc cggcaccgtg
    11341 gagtttctat tagatcccga tggacgttac tacttcatgg aaatgaaccc tcgtatccaa
    11401 gtggaacata ctgtcacgga acagattact accgttgata ttgtgcaatc ccaactatgg
    11461 attgcggcag ggcgtcctct ggtcttcggg cagcatgagg tcaatctcca gggctatgcc
    11521 attcaatgtc gggtaaatgc tgaagatccc caaaatggat ttcgaccatc atcgggaaaa
    11581 attaccgcct atttgtcccc gggtggtgtt ggagttcgga ttgacggagc ggtgtacaag
    11641 gattataccg tccctccgta ttatgatgcc ctcctcgcca agttgaccgt tcatggtcgg
    11701 acctgggacg aaactgttcg ccgaactcat cgctcccttg aggaatttgt cctgcgaggt
    11761 gtcaaaacta ccattccatt tatggcaaaa ctcatggaag aaccggattt ccgggccggg
    11821 cgatttgata cctcctatct tgaacgccat cccgaacttt ttgaatatga agaggcggaa
    11881 gaaccggaag atctcgtcat tgccctatcc gcagctatcg ccgcatttga aggattataa
    11941 caccctttat gaaacgttat gatccattgc ccatgtgaaa ggtctcctta tgtcacgaaa
    12001 aacatcacac acccataaaa gcaagaagcc cagtcgtccg gtccctacca ggatgaaatt
    12061 ggatgccgct ccggccctgg atatcgaagc gtctccgaag cggcaggtct ttttaacaga
    12121 tgtggctcta cgagacggac accagtccct tttagcgacg cgtatgcgca cagaggatct
    12181 cttatccgtg gcttcagaat tagacgaagt cggattttgg tcattagaag tgtggggcgg
    12241 cgcaaccttc gattcgtgtc tccggttcct taaagaggat ccttgggagc gccttcgagc
    12301 ctttcgtgaa gccatgccga aaactcgtct tcaaatgctc ttgcgtgggc aaaaccttgt
    12361 agggtatcga cattatgctg acgatgtcct tgaaaaattc atcgaattat cggcgcaaaa
    12421 tggtattgat gtctttagaa tctttgatgc cctgaatgac atcaggaaca tcaaaccggc
    12481 catggaagtt gtgaaggcct gcggcaagca tattgaagcg actatttgtt atacggtcag
    12541 tccggtccac agtctgaatc actttgtgga acaagctaaa caactcgaag acctcggggc
    12601 agacaccatt tgtatcaagg atatggctgg acttcttcct ccctttgaag cctatgaann
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cccggctgca gataaattac
    12781 gcaaagcccg taaacgctat cggcagtttg aaagcgattt caccggtgtg gatacggaca
    12841 ttctcctctc ccaagtgccc gggggaatgc tttcgaattt agcagcccaa ctggccgaac
    12901 aaaatgcgct gggaaaaatc aaagaagtgc tggaagaaat tccacgggtg cgaaaagaca
    12961 tgggatatcc ccctctggtc acccccacga gccaaattgt cggaacgcag gcgacgctga
    13021 atattttgac cggcgagcgt tataaagtga ttacgaatga aaccaaaaac tattttcagg
    13081 gtttgtatgg gaaaccacct ggtaccctca actcgaagat tcggcaaaaa gcattggaag
    13141 gcgacgtgcc tgtagccggt cgtcccgctg acaaccttga tcctgagctg ggagatgcaa
    13201 cgcaggaact cgtaggacgg gaaatggctg aagaggacat tgtctcctat gcgctcctcc
    13261 cgtccgtggc cttgcaattt tttgatgaac gggagcgagg ggaactcaaa cccgaacccc
    13321 ttcacgcctc cccggagagt gggccagcgg tggctcacga tctacattta gctccggtag
    13381 aattcaacgt caccgttcac ggcgaagcct atcatattcg cgtgtcggga tccggccaga
    13441 cagtagacgg cgtgaaaccc tattacatta aggtgaatga caaacttgaa gaagtgtatc
    13501 ttgaaccgat tcaagaagtc ttagccggat cacctgaccc tccaccatcc caggcatcaa
    13561 agacggagaa acgtcccaag ccatcacaac ctggagacgt gaccacaccc atgcccggcc
    13621 gggtggtcaa agtcctggtg acagagggtt ccactgtcaa cgtgggtgat tccctgctgg
    13681 tagtggaagc catgaaaatg gagaatcgag tccaatcgcc gattgccggt tccgtcatca
    13741 ccatctacgt caaagaagga gatgaagtga atcctgatga aacgctgatt catctggaat
    13801 aataatgcgg ggctgttctc actttcgatt atttctcctt ttcatggtcg tgctaggatg
    13861 cttgacgatt ttgggttgtt cttccctcaa gacgctccct tccggaatcg acctgatcca
    13921 acgtgtgccc tatcagacca ccagagtcaa cacccatcgt attgcctatc ttgacgttgg
    13981 gcaaggtccg ccgcttatcc tcatccacgg atttggcgga tcgatgtggc aatgggagca
    14041 tcaatattcg gttctagccc aaactcaccg ggtcatcatc ctggatttgc tcggttctgg
    14101 gctctctgat aaaccggatg atgcgtatac gccaaaatat ctcgtggagt tttttcgcca
    14161 attcatggat gccctcaata tttcacgagc gacgttagtc ggaaattcca tgggagccgg
    14221 gttggcgatg gccatggccc ttgactatcc ggaacgagtg gatcgacttg tgctcatctc
    14281 gggatttccc gcccacgttg agaacagcat cgcttcacca cattaccaac gctttctcca
    14341 ccaccgccct cccatttggc tcgcaaaatt aggaaactgg atggccggga gaaggaccac
    14401 agaatatatt ctcaaagaaa ttatctataa tgccgcactc atctcccctg ccattattga
    14461 ccgttcattt cacaatcgac aaagaggcga ttttcttgtc cctctttatt cattgatgga
    14521 caatatcaaa acatgggagg gacaatatgg aaataggttt cagcagattt ctcatcctgt
    14581 cctgctactc tggggagatc aggaccgagt gtttccgctc gatgtgggtg aacgggtaaa
    14641 agaccaatta cctcatgtcg aatggcatgt catcccggaa gcggggcatc ttgcccagtg
    14701 ggaagcaccc actatggtga atcaatttat cctctcattt ttagaacgag atttctaaag
    14761 gacacatcac tcaaccgggt atttatccca tgcgtatatg agaaaatggt attctttata
    14821 taacaccgat agcttaacct ggatcatcga gttggataga aggagcatca tcatgcaaaa
    14881 aaattcctcc attatgtcgt tactttttat cctagcggct tcctcattag ctggatgttc
    14941 caccctgaat ggccaatcag accagaattt ttcctataaa aacattccga ttgataaaga
    15001 aaccgccagg acaggagaag ggtcgaccat tctttttcga ggtaccccct tgccgctgag
    15061 tggtatggaa gtgaaaaccg gggagactct tcgtgccgtc ccattggcaa aaggtgactt
    15121 atcactcgtg aacatccatg aatcccaagg gaaagtccgc atcatcagta tcgtgccttc
    15181 cctcgacacg aaagtttgcg aacaacaaac acattacctg agtgaaaaaa acaagggtct
    15241 tgatcagcag gtgtcactga taaccattag cgtcgatacg cctttcgctc aagagcgatt
    15301 tgcgaaagaa gcaggaattg ccaatgttga atttctgtca gattttcgag gtggagagtt
    15361 tggcatctcg catggattac tcttggagag accccatgtc ctggcccgag ccgtcatggt
    15421 tgtcgatgcg aataatgtga tccgcta
//
LOCUS       DAORAQ010000059        15386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_656_length_15386_cov_71.445503, whole genome shotgun sequence.
ACCESSION   DAORAQ010000059 DAORAQ010000000
VERSION     DAORAQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_15386_cov_71.445503"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..300)
                     /locus_tag="PKM72_07260"
     tRNA            complement(223..300)
                     /locus_tag="PKM72_07260"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Glu,seq:ttc)
     gene            complement(557..981)
                     /gene="rnpB"
                     /locus_tag="PKM72_07265"
     ncRNA           complement(557..981)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKM72_07265"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1053..1781)
                     /locus_tag="PKM72_07270"
     CDS             complement(1053..1781)
                     /locus_tag="PKM72_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNP60622.1"
                     /translation="MNLQLQYLINLQKFDLRIFQIQDQLRKAPELLKFAESPLQDILT
                     RLQVLKNTGESLVKQRRSSERELATQEEQLQKIRNRLSELKTNKEYQAHLFEIELARK
                     KKDSIEENVLEMMERVEQNEQAIKELEEQSTEAQKVFDTEKARLETHFANLENELTDL
                     NRQQTTLSVLVDKPLLVRYNRLKTLRKGYAVAQLREGACGGCQLQLPPQLVAEVKRGD
                     ELMDCSYCHRILYLARHLDEETTE"
     gene            complement(1844..1920)
                     /locus_tag="PKM72_07275"
     tRNA            complement(1844..1920)
                     /locus_tag="PKM72_07275"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1884..1886),aa:Ile,seq:cat)
     gene            complement(2011..3837)
                     /gene="dnaG"
                     /locus_tag="PKM72_07280"
     CDS             complement(2011..3837)
                     /gene="dnaG"
                     /locus_tag="PKM72_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNP60623.1"
                     /translation="MTGEKRGVPPEILQQIRDRIDIIDLISTYVSLSKAGQNFKGLCP
                     FHSEKTPSFSVNPVRQMFYCFGCSVGGDAFTFLMKQEGMDFMEALRELSQRTGVALPE
                     RRESGAKPSSGLSRERYFHVYQLAASWYHRNLLETPEGKVARDYLDRRGIARETWSTF
                     QLGFVPEGWNGLSKWLEQQSVKPEELVQAGLLARKESEDGTRVSTYDRFRGRVMFPIT
                     DPRGQVLGFGGRILEDGASPKYLNSPETDLFFKGRSLYGMDKARQSATASGRFYLVEG
                     YFDVIVLHQNGIENAVAPLGTALTADHVQILRRLAPSVMLVFDGDAAGIGAALRTLDL
                     FMNSGIEVRVLLLPAGEDPDTFIRKNGVSAFRELEGKAATLLDFAIMSVLNKAKMHSI
                     QDRVKRADEILAILQKTKNPIEKEEYLKVVSERLGIRPDLLSKRLPTLSRPMNAAPSV
                     KRQEQAVTNLAIPQGKPEERDLIVLLLQGRLESNQIRQLDEDAFTVPLYRHILRKAFQ
                     HTDEEGNVNLDALHAEFSGDPAYEPVISRLSVAEYYCEEVFVHVVGCLRVLKEKQIQR
                     LMDEVISQLKVAQREGRQDLVDALVAESNVLRQKKSLLTASP"
     gene            complement(3937..4281)
                     /locus_tag="PKM72_07285"
     CDS             complement(3937..4281)
                     /locus_tag="PKM72_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNP60624.1"
                     /translation="MGDCLFCQIVKGAIPADTLYEDEHTFVFNDINPQAQVHMLVIPK
                     RHVVSLDDTKETDAALLGHLMVVCAKMARQREISGSGYRVVANTGPGAGQSVFHLHFH
                     VMGGRTFSWPPG"
     gene            complement(4274..4951)
                     /gene="hisIE"
                     /locus_tag="PKM72_07290"
     CDS             complement(4274..4951)
                     /gene="hisIE"
                     /locus_tag="PKM72_07290"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNP60625.1"
                     /translation="MSPLQFDEQGLLPCVVQDWLDGTVLMVGFMNQQAWEATCESQRV
                     HFWSRSRKRLWKKGETSGHELLVKEMFVDCDRDTVLVKAQPLGPTCHTGNRSCFFSPM
                     PSSTSEEKAAILSGTAWGGITRRLYEMVDDRKTNPSPDSYVSKLMGGGLDRILKKVVE
                     EAGEVLLAGKNGDREEIIYEMADLWFHTLIMLGHFSIPPEEIYQELGKRFGKSGIRQP
                     EGDPTHG"
     gene            complement(4976..5773)
                     /gene="hisF"
                     /locus_tag="PKM72_07295"
     CDS             complement(4976..5773)
                     /gene="hisF"
                     /locus_tag="PKM72_07295"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNP60626.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVHVAQVYDREGA
                     DELCFLDITASHEKRDTILDVVIRTAEQVFMPLTVGGGIRTIEDIRRLLEAGADKVSI
                     NTAAVEHPEFVKAAANRFGSQCIVVAIDAKRATTCEGGEPGWEVFTHGGRNSTGLDVI
                     EWGKRMRDYGAGEILLTSMDRDGQQDGYDLALTEAISESVSIPVIASGGAGTLQHLYE
                     AFHVGKADAVLAASIFHYQTYSIGQAKSYLAQQSIPVRLGTVPVSIS"
     gene            complement(5767..6513)
                     /gene="hisA"
                     /locus_tag="PKM72_07300"
     CDS             complement(5767..6513)
                     /gene="hisA"
                     /locus_tag="PKM72_07300"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNP60627.1"
                     /translation="MLLIPAIDLKDGKCVRLRQGEMDQVTRYSDDPVAMAEHWQGLGA
                     RYLHVVDLDGAVTGTPQHLSHIEAITKRLAIPLQVGGGIRNTSTIRAYLSCGVDRVVI
                     GTAALQDAEFLAAAAKEFPSKVLIGIDVKQGLVAVHGWKTVSSLTPQQVFESVKDLPL
                     GGMVFTDISRDGMLAGPNISALRDAVAISPFPVIASGGVTVLKDVQTIQNLGSKISGI
                     IIGKALYEGTLDLKAALEMVASAEASRTSC"
     gene            complement(6513..7319)
                     /locus_tag="PKM72_07305"
     CDS             complement(6513..7319)
                     /locus_tag="PKM72_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60628.1"
                     /translation="MRGVCLAINGRSTPLPARFLVISLIGWWCFGVGCVPSKVQVQAS
                     PEFDPSRITSLAILPFQTIKTPQLGSSSIRGGVRDPEEIRTQFRLPGTDQGNGADSRK
                     VRFVVPATAAHRITTQVVSVLANRPSLRVIGPEESSAVLGRGSQEGERSFSVMAQEVG
                     TQLKVDGVLTGLVSTYREREGSSLGANPAAVGFEVYLVRPSDGTILWTGEYFEEQKPL
                     NQDVVGFFEKGGGFVTAEKLSELGVQKVMKAFPVGLADPGPARSLREGSD"
     gene            complement(7316..7939)
                     /gene="hisH"
                     /locus_tag="PKM72_07310"
     CDS             complement(7316..7939)
                     /gene="hisH"
                     /locus_tag="PKM72_07310"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNP60629.1"
                     /translation="MIAIIDYGMGNLRSVQKGFECVGHEAVVTRDPRVIDQASHVVLP
                     GVGAFGDCMDNLVRFELTDVIHRSISSGKPFLGICLGYQLLFSESEEFGCHKGLGILA
                     GKVKAIPRPSDGGEVSFKIPHMGWNTVQVNRAAPPLRGIPSDSYVYFVHSYYVEPADG
                     ALVSTETEYGIPFASSVWKDNIFATQWHPEKSQAVGLQVLKNFGDWQ"
     gene            complement(7984..8586)
                     /gene="hisB"
                     /locus_tag="PKM72_07315"
     CDS             complement(7984..8586)
                     /gene="hisB"
                     /locus_tag="PKM72_07315"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNP60630.1"
                     /translation="MNQHLVPRTASQERKTAETAIRVQWGLDGSGNNQIKTSIPFLDH
                     MLNLFGKHGFFDLMVEAKGDIEIDDHHTIEDVGIVMGQVLTKALGTKEGIARFGWAAV
                     PLDETLAQVTVDLSGRPYLVYNVALPARYIKTFDLGLFEDFFQAFVSQGGLNLHVNVP
                     YGRNPHHIMEAIFKAFAKALDQAVKREERLQGVLSTKGSL"
     gene            complement(8583..9866)
                     /gene="hisD"
                     /locus_tag="PKM72_07320"
     CDS             complement(8583..9866)
                     /gene="hisD"
                     /locus_tag="PKM72_07320"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNP60631.1"
                     /translation="MKIVSHKDQGFSQAVDRVCQRGAQQNTKVEKVVRTILEAVRQEG
                     DQALQRLTYKFDHVRISPDGLRVTPKDVERAYAELPSSDTKALKYAANRIRTFHRRQR
                     RASWVYRKEGIQLGQRITSLDAVGVYVPGGKALYPSSVLMNAIPAKVAGVKRVVMCTP
                     TPTGIIHPALLVAADVAGVDEIYRVGGAQAIGALAFGTSTIRPVDKIVGPGNLYVATA
                     KRLVYGQVDIDMIAGPSELLVIADSASNPLHVAADLLCEAEHDEEACVYLVTTSLTLA
                     RRVVTEVTSQLAQLGRQAIATRSIQNHALAFVVRTFDDAFELANRIAPEHLSLNVDRA
                     GDYVSRIRHAGAIFVGRYTPPAVADYVAGPNHVLPTGGSARFFSCLGVDEYMKTSNVV
                     TYSQAALRKANASVMRLSKLEGLDAHGRSMESRWT"
     gene            complement(9863..10525)
                     /gene="hisG"
                     /locus_tag="PKM72_07325"
     CDS             complement(9863..10525)
                     /gene="hisG"
                     /locus_tag="PKM72_07325"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNP60632.1"
                     /translation="MSDVITIALSKGKLLDPTLELFRQAGYTGYHVRADDRRLILEFS
                     EHGHRVLIVRPSDVPTYVEYGAADLGVVGKDVLLEQSPDVYEPVDLNLGVCRLVVARP
                     NGPQLGQRMSSKLRVATKYPTITERYFNQQGLPVELIKLYGSIELAPLVGLADRIVDL
                     VETGSTLKANNLVEEEVIAWSSARLIVNRASLKMKHAVITELIARVKKILSRSRSKKV
                     HV"
     gene            complement(10522..11781)
                     /gene="murA"
                     /locus_tag="PKM72_07330"
     CDS             complement(10522..11781)
                     /gene="murA"
                     /locus_tag="PKM72_07330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNP60633.1"
                     /translation="MDQIRITGGLPLKGAVEIGGAKNAALPILAATLLGGGECVIDHV
                     PRVRDVMTMGKLLALLGVTVQEEGDRIRLDAAQVQSIEAPYDLVKTMRASILALGPLL
                     ARLGEAKVSLPGGCAIGTRPVNLHLKGLEMMGAEIQVEHGYIHAKACRLKGARIDLDF
                     PTVTGTENLIMAACLAEGTTSLHNVAREPEITDLCAFLTKRGAKIHGTGTDTITIEGV
                     KELHGAGHWVIPDRVEAGTYLMAGAITGGNVMVKGCMTDHLGSVLKKIQEMGAELTEC
                     HDGVRICRSGPLKAVEMKTLPYPGFPTDLQAQMMALMSVAEGVSVISETIFEGRFLHV
                     PELHRMGASIEIDGPHAVVKGVPLLTGAPVMASDLRASAGLVLAGLAADGETTVSRVY
                     HLDRGYERLEEKFQALGAKIERIREVA"
     gene            complement(11798..12676)
                     /gene="prmC"
                     /locus_tag="PKM72_07335"
     CDS             complement(11798..12676)
                     /gene="prmC"
                     /locus_tag="PKM72_07335"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNP60634.1"
                     /translation="MPTFTSYSQLYRGAVETLTQAGIANARHEALWILEDALGITRLQ
                     LHANPDTPVEADVCQRAVALFQRRALHEPLQYVLGTQDFCGLDFLVQPGVLIPRPETE
                     LLVEEALGLLGGDARPVILDVGTGSGCLAISLAIHLPGAMIIASDRSVTALHLAQINA
                     ARHGVSQRILWVAGDLLSHLLSRNLAGKVTAIIANLPYISHAEWEHLSPDVKDFEPRL
                     ALDGGPDGLDVYRRLLDESPGLVSSKGFILMEVGVGQADLLCRDPSLTNAFKTVKVRP
                     DSQGIPRVVCVQQRLV"
     gene            complement(12746..13825)
                     /gene="prfA"
                     /locus_tag="PKM72_07340"
     CDS             complement(12746..13825)
                     /gene="prfA"
                     /locus_tag="PKM72_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNP60635.1"
                     /translation="MSAVTKAELKEGGLFSKWEAIANKYDTLNDQLSDPAILSQSTLL
                     VALNKERAELEPIAQLFGGYRRVVEEIAQARLMTEDLQLDPDMRRLAGEELQSLESQR
                     EAMEAQVIELLCPADEGDNKNSFIEIRAGTGGSEAALFAGDLLRMYTKFAEAKGLRVE
                     LMEFQETGLGGVKEAVLLVEGKGAFGNFKYESGVHRVQRVPTTEANGRIHTSTATVAV
                     MPEVEEVEVHIDPKDLRIDTYCSSGAGGQSVNTTYSAIRITHIPTGVVVTCQDERSQL
                     KNRTKAMRTLRTRIGDAEREKQEASIAEQRRSQVGTGERSEKIRTYNFPQNRVTDHRI
                     GLTLHKLDRVMEGDLNGLIAGLKAQ"
     gene            complement(14094..14315)
                     /gene="rpmE"
                     /locus_tag="PKM72_07345"
     CDS             complement(14094..14315)
                     /gene="rpmE"
                     /locus_tag="PKM72_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNP60636.1"
                     /translation="MKKGIHPAYEFANVSCACGMTYQTRTTVGDLKVDICSSCHPFFT
                     GTQKIVDAEGRVERFNKKYAKLTKPKAKA"
     gene            complement(14392..>15386)
                     /gene="rho"
                     /locus_tag="PKM72_07350"
     CDS             complement(14392..>15386)
                     /gene="rho"
                     /locus_tag="PKM72_07350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNP60637.1"
                     /translation="RRFNLRTGDIVSGQIRPPKDGERYFALLKVEKINYDEPEVQRDK
                     ILFDNLTPLYPEERINLEFDPEEYCTRVMELTTPIGKGQRGLIVAAPRTGKTMLLQAV
                     ARSIIANHPEIVLIVLLIDERPEEVTDWQRQVKAAEVISSTFDEPPQRHAQVAEIVME
                     KAKRLVEHKRDVVILLDSVTRLARAYNTISPPSGKVLSGGLDSNALQRPKRFFGAARN
                     IEFGGSLTILATALVDTGSRMDDVIFEEFKGTGNMEVHLDRRLADKRLFPAIDISQSG
                     TRKEELLVDRDRLNKMWILRKVLSPLGTMEAMEFLMDKIGGTKSNNEFLQSMNR"
BASE COUNT         3871 a         4435 c         3390 g         3690 t
ORIGIN      
        1 gcgtttctaa aaggtcaggg caaagagcgg aaaatacatg aggggacttg ctacgggtgc
       61 cacgtcctca gggtctttat atggccttaa gggcgaagac ttaaacgcaa atatgagctt
      121 aatatgcttg aaatacgaaa gaaaccggac gattcagcaa aaattcattt ctgaatcctg
      181 ccaacattcc tcgtggtact gactacggaa aaaaatagga agtggcgtcc ccaacgggat
      241 tcgaacccgt gttgccggct tgaaaggccg gcgtcctagg ccaggctaga cgatggggac
      301 gcaatatttc aggataggta tttggccttt tatgaacaat actgaagcga tggacccggc
      361 attctatcaa tgaggaaaac acgagtcaaa ccaagaagtt attctcaacc atttagatgc
      421 catcagctaa cagaaccata cgtggcttcg tcggcactat tgtctcctcg cattcgtgaa
      481 acctttcaga ccagggctca tcaatcggta aaaaaaccac cacaattttt gacaaccact
      541 taaaccgtaa agatggtgaa ggagtgggcc tgtaagccgg attctgtact ggatgaaacg
      601 agtttcatcc aatggtgacc atttctctgg aacctaggtt accctaggct tcaagcaacc
      661 tacccgagaa cctcgaccgg gccggtctct ctcccggttc gtgaagaaat cgcgtccttc
      721 gacaaaccgg gaatcgtccc cctatttggt tttgctccag gtgacgctta ccctgccacc
      781 tgtgtcacca caggcgcggt gggctcttac cccaccattt cacccttacc tggcaaaaac
      841 cgaagaattt gccattggcg gtatattttc tgtggcgctg gtgtcggatc actccgcctg
      901 ggcgttaccc agcaccttgc cctttggagt ccggactttc ctctcacgga aaattccgtg
      961 agcggccacc cggcccactc gcaccatcta tagctagtta tcgtaggttg agaaaaggat
     1021 gaaagcaaga gacgcggcgc atatagaaat agttattcgg tcgtttcttc atcaagatgc
     1081 cgggccagat agagaatgcg gtggcagtag gaacaatcca tgagttcatc accacgcttc
     1141 acctccgcga ccagttgagg aggaagctga agctgacacc ccccacaggc tccttcacga
     1201 agctgggcaa cagcataccc ttttctcaat gtttttaatc ggttgtatcg aaccagcagt
     1261 ggcttgtcca ccagtacgga gagtgttgtt tgctgacggt ttaaatcggt taactcattc
     1321 tccaaattgg caaaatgcgt ttccagtctg gccttttcgg tatcaaaaac tttctgggct
     1381 tcggtgcttt gctcttcaag ctccttgatg gcctgctcgt tctgttccac ccgctccatc
     1441 atttccagca cattctcctc aatcgaatct tttttctttc gagctaactc tatttcgaag
     1501 agatgcgcct ggtattcttt gttcgttttt aattcagaaa gccgatttct gattttctga
     1561 agttgctctt cttgagtagc gagttcccgt tccgaacttc gtcgttgttt gacgagagat
     1621 tcaccggtat ttttaaggac ttgcagtcgg gtgagaatat cttgaagcgg agattcagcg
     1681 aacttgagaa gctctggtgc cttccgcaat tggtcttgaa tctgaaaaat tcgaagatca
     1741 aatttttgaa gattaatcag gtattgcaac tgcaaattca cagagtcctc aatatcctaa
     1801 atcacgttta cccacccggt cattcaagtc cggccctcgc tcatggtggg cccaccagga
     1861 cttgaacctg ggaccaactg attatgagtc agccgctcta accaactgag ctatgggccc
     1921 gagatcataa agccgcacac actcttttac cataaatggt gctcaggaaa aactacccct
     1981 acgaatttta ttccatgaga gggtccaaat tcatggagaa gctgttaata gggacttttt
     2041 ctggcgaagt acattggact ccgcgaccaa agcatcgacc aagtcctggc gtccttcccg
     2101 ttgcgcaacc ttgagttgac taatcacctc atccattaga cgttggatct gtttttcctt
     2161 taaaacacgg agacatccga cgacatgaac aaacacttcc tcacaataat attccgccac
     2221 actcaaccgg gaaatgaccg gctcataagc cggatcccca ctgaattccg catgcagtgc
     2281 atcaaggttt acattccctt cttcatccgt atgctgaaaa gccttgcgaa gaatatggcg
     2341 atacagtggg acagtaaacg catcctcatc gagttgacga atctgattcg attccagtcg
     2401 tccttgaagt aataacacaa taagatcccg ttcctccggc ttgccttgag gaatggccag
     2461 attcgtgaca gcttgttcct gtcgcttaac agacggggcg gcattcatcg gccgggaaag
     2521 cgtcggaagg cgtttactga gtaagtccgg tcgaataccc aatcgttccg agaccacttt
     2581 cagatattct tctttttcta tgggattttt ggtcttttga agaatcgcaa gaatttcatc
     2641 agcccgcttg actcgatctt gaatggaatg cattttggct ttgttcaaga ccgacatgat
     2701 ggcaaaatcc aacaacgttg cggccttccc ctccaactca cgaaacgccg acaccccgtt
     2761 tttccgaata aatgtgtcag ggtcttcgcc agccggaagc agcagcaccc ggacttcaat
     2821 gccggaattc atgaacaaat caagcgtccg taacgccgcg ccgatgcctg cggcatctcc
     2881 atcaaaaaca agcatgaccg acggggccaa gcggcgtaaa atctggacat gatcagcggt
     2941 taacgccgtc cctaatggtg ccacggcatt ttcaatcccg ttttggtgaa gaacgatgac
     3001 atcaaaatac ccttctacca aataaaacct tccggaagcg gtggctgatt gtctggcctt
     3061 gtccattcca tagagggatc gccccttaaa gaacaagtcg gtttcgggtg aattcagata
     3121 cttgggagag gccccatctt ccaggatccg cccaccaaat cccaatactt gtccacgcgg
     3181 atcagtaatg ggaaacatca cccggccacg aaatcgatca tacgtggata ccctggtgcc
     3241 gtcctcagac tctttccgcg ccagcaatcc agcctgaacc agttcttcag gcttaacgga
     3301 ttgctgttcc agccatttgg aaaggccatt ccatccttcc ggaacgaatc ccaactggaa
     3361 tgtgctccag gtttccctgg caatcccccg tcgatccaaa taatctcgag ccaccttccc
     3421 ttcaggagtt tccagaagat tgcggtgata ccaggatgct gccaattgat acacatgaaa
     3481 ataccgctca cgagataagc ctgaagaggg cttcgcccct gattcccgac gctcaggcag
     3541 agccactcct gtccgttggc ttaactcacg cagagcctcc atgaaatcca tgccttcttg
     3601 tttcatcaaa aaggtaaagg catctcctcc gacactgcag ccaaaacaat aaaacatttg
     3661 tcgaacgggg ttgaccgaaa atgagggggt tttttctgaa tgaaaggggc acagcccttt
     3721 aaagttttgc cccgccttgg aaagactcac gtaggtggaa atgagatcaa taatatcaat
     3781 gcgatctcga atttgctgaa gaatctctgg agggacccct cgtttttcgc cagtcatctc
     3841 acttctcacc acaaactgtg ttagggacac atcacgaatt tcattccctt gggataccaa
     3901 atattatcgg agcgcttttt ccccggcaag ccggcattaa cccggaggcc acgaaaatgt
     3961 ccgcccaccc atgacgtgaa agtgcaaatg aaagacactc tgcccggctc caggtccggt
     4021 attcgcaacg acccgatatc cgctaccgga aatttcccgc tgtctggcca tcttggcaca
     4081 gacgaccatc aagtggccca ataaggcagc atccgtctct ttcgtatcat ccaaagacac
     4141 cacatgccgc ttgggaatca ccaacatatg cacctgcgct tgaggattga tatcgttaaa
     4201 cacgaacgtg tgctcatcct catacagcgt gtcagcggga atggccccct tcacgatttg
     4261 acaaaaaaga caatcaccca tgagtagggt ctccttccgg ctgtcgaatt cctgatttgc
     4321 caaatcgctt gccaagctct tgataaatct cttcaggagg aatggaaaaa tgcccgagca
     4381 tgatgagggt atgaaaccac aaatcagcca tttcataaat aatttcttcc cgatcgccat
     4441 tttttccggc cagcaggact tcgccggctt cttccaccac ctttttcaaa atacgatcga
     4501 ggcctcctcc cattaatttc gacacatagg aatcaggact cgggttggtt ttccgatcgt
     4561 cgaccatttc atacaggcgt cgggtaattc cgccccaggc cgtcccggac aggatggcgg
     4621 ctttctcttc agaagtcgaa gaaggcatgg gagaaaaaaa acatgaacgg tttccggtat
     4681 gacacgtcgg tccaaggggt tgggctttca ccaagaccgt atcccgatca caatccacaa
     4741 acatttcctt cacgagtaac tcatggccgg acgtctcgcc ttttttccat aaccgttttc
     4801 gggatcgact ccagaaatga acccgttggg attcacacgt cgcttcccat gcttgttggt
     4861 tcataaaccc caccatcaac accgtcccat ccaaccagtc ctgcaccaca caggggagca
     4921 atccttgttc atcaaactgc aatgggctca tcgaagagac ttcgctcatg gttggttatg
     4981 aaattgaaac cgggacggtt cccaggcgaa cagggatact ttgttgagcc aaataggatt
     5041 tggcttgccc gattgaatac gtttgataat gaaaaatcga tgccgccagc accgcatcag
     5101 ccttccccac atggaacgcc tcatacaggt gctgaagggt gccagcccct ccagaagcaa
     5161 tgacgggaat ggagacggac tcggaaatag cctcagttaa agctaaatca tagccgtcct
     5221 gttgtccatc ccgatccatg ctggtcagaa gaatttcccc ggctccatag tccctcatcc
     5281 gcttccccca ctcaatgaca tccaggccgg tcgagttccg ccctccgtgg gtaaagactt
     5341 cccaaccggg ttcacctcct tcacacgtgg tcgcccgttt cgcatcaatc gccacgacga
     5401 tacattgcga tccaaaacga ttggcagcag ctttcacgaa ttccgggtgc tccacagcag
     5461 ccgtattaat gctgactttg tccgcacccg cctctaataa tcggcgaata tcctcaattg
     5521 tccggatccc tcccccaacc gtgagaggca taaacacctg ctccgccgtc cgaatcacca
     5581 catctaaaat cgtatcccgc ttttcatgtg aagcggtaat atccaaaaaa cacagctcgt
     5641 ccgcaccttc acgatcatac acctgcgcca cgtgaaccgg atctcccgca tcacgcaaat
     5701 tcacaaacga gacccctttc acgactcgcc cgtctttgac atccaggcag ggaataattc
     5761 tcttcgtcag catgatgttc gagacgcttc tgcagaggcc accatttcaa gagccgcttt
     5821 caaatccaag gtcccttcat acaaggcctt tccaataatg attccggaaa tcttgcttcc
     5881 aaggttctga atggtctgta catccttcag taccgtcact ccaccagagg caataacagg
     5941 aaacggcgag atagccactg catcccgtag agctgaaata tttggccctg ccaacatgcc
     6001 gtcccgagaa atatccgtaa agaccatgcc tcccaaaggt aaatccttta ccgactcaaa
     6061 gacctgttgt ggggtcaggc tggacacggt tttccatcca tgcaccgcga ctaaaccctg
     6121 cttgacgtca atcccgatta acactttaga aggaaactct ttcgcggctg ccgccaagaa
     6181 ctcagcatcc tgtaaggccg ctgttccaat caccacccgg tcaaccccac aggacaagta
     6241 ggcacgaatc gttgacgtgt ttctgatgcc cccaccgacc tgcaggggga tcgccaaccg
     6301 tttggtgatg gcttcgatgt gagacagatg ctgaggtgtt ccggtcaccg ccccgtctaa
     6361 atccaccaca tgaaggtagc gggcaccaag accctgccaa tgttccgcca tcgccacggg
     6421 gtcatcggaa tacctggtca cctgatccat ctcgccctgt cgcaaccgga cacatttccc
     6481 gtccttcaga tcaattgcag gaatcagtag cattagtcag agccctctct caaagaccga
     6541 gcagggccag gatcggccaa accgaccgga aaggccttca tcactttttg tacccccaat
     6601 tcggaaagtt tctcggcagt cacaaacccg ccaccttttt caaaaaaccc taccacatcc
     6661 tggttaagag gtttttgttc ttcaaaatat tcccctgtcc acaagatggt tccatccgaa
     6721 ggcctcacga gatacacttc aaagcccacc gccgcagggt tagccccaag gctactccct
     6781 tcgcgttccc ggtatgtgct aacgagcccc gtcaggactc catcaacctt taattgcgtc
     6841 ccaacctcct gggccatgac cgaaaatgac cgctcacctt cctggcttcc cctgcctaac
     6901 acggcggagg actcctctgg gccaatcacc cgtaaagaag gccggttggc caacacagaa
     6961 actacctgtg tcgtaattcg atgtgcagcc gtggcaggca ccacaaaacg aacttttctg
     7021 gaatctgctc cattcccttg atcagttccc ggtaaccgaa attgtgtacg gatttcttcc
     7081 ggatccctca cgcctccacg tatcgagcta ctccccaatt gaggcgtttt gatcgtctga
     7141 aaaggaagga tggcgagact ggttatacgg gagggatcaa attcaggaga agcctgaacc
     7201 tgaacttttg agggaacaca gcccacaccg aaacaccacc acccgatcag gctgatgact
     7261 agaaacctgg caggcaacgg tgttgaccgt ccattgatcg caagacacac tcccctcatt
     7321 gccaatcccc gaaattcttc agaacctgca gtccaacggc ctgacttttt tcgggatgcc
     7381 attgggtcgc aaagatattg tctttccaga cactacttgc aaagggaatt ccatattcgg
     7441 tttcggtact gacgagggca ccatcagccg gttcaacata gtaggaatgc acaaagtaca
     7501 cataggagtc cgagggaatg ccacgtaagg gcggagcggc cctgttgacc tgaaccgtat
     7561 tccagcccat atgaggaatc ttgaacgaca cttccccacc atcggacgga cggggaatcg
     7621 ctttcacctt tcctgcaagg atcccaagtc ctttatgaca cccaaattct tcactttccg
     7681 aaaacaataa ttgatagcct aaacaaatgc cgagaaatgg ttttcccgag gaaatagaac
     7741 gatgaatgac gtccgtcagt tcaaagcgaa ccaggttgtc catgcaatcc ccaaacgcgc
     7801 ccacacccgg taacaccaca tgactggcct gatcaattac gcgagggtct cgagttacga
     7861 ccgcttcatg ccccacacat tcaaaccctt tctgcacgct ccggaggttt cccatcccat
     7921 aatcaataat ggcaatcatc tttcggtcgc ttccttctct gccgatccac tcattgtcgc
     7981 catttataac gatcctttag tggagaggac tccctggagc cgctcttctc gttttacggc
     8041 ctggtccaag gctttggcaa aggctttaaa aattgcctcc atgatgtgat gaggatttct
     8101 tccgtaaggg acattcacat gaagattcaa ccctccctga ctcacaaaag cctgaaaaaa
     8161 atcttcaaac agccccaaat caaacgtctt aatatacctc gcaggaagcg ccacattgta
     8221 cacaaggtac ggccggccac tcagatcgac cgtcacctga gccaacgttt catccaacgg
     8281 caccgctgcc cacccgaacc ttgcaatacc ctctttcgtg ccaagagcct tggtgaggac
     8341 ttgccccatg acaatgccca catcttcaat cgtgtgatgg tcatcaatct cgatatcccc
     8401 tttcgcttcc accatcagat caaaaaatcc atgtttaccg aataaattca acatgtgatc
     8461 taaaaaggga attgaggtct taatctgatt attgccactc ccgtcgaggc cccattggac
     8521 tcgaatagcc gtttctgcgg tttttcgttc ctgagaagcc gttctgggaa caagatgttg
     8581 gttcatgtcc atcgactctc cattgatcgc ccatgtgcat ccaggccttc cagcttagac
     8641 agccgcatca ctgaagcatt ggctttgcgc aacgcggctt gcgaataggt caccacgtta
     8701 ctggttttca tgtactcatc cacacccaaa cacgaaaaaa atctggcact gcccccggtt
     8761 ggtaggacat gatttggccc tgccacataa tccgccactg caggaggggt atagcgccca
     8821 acaaaaatgg cacccgcatg acgaatacgg gacacatagt ctcccgcccg atccacattt
     8881 aatgataaat gctcaggcgc aatccggttg gctaattcaa aggcatcatc aaatgttcgc
     8941 acaacaaaag ccagagcatg gttttgaatt gatcgggtcg caatagcctg gcgtcccaac
     9001 tgtgcgagtt ggctggtcac ttccgtcaca acccgtctgg ccagagtcag agaagtcgtc
     9061 accagataga cacaggcttc ctcatcatgc tcagcctcac aaagaagatc ggccgcaaca
     9121 tgaagcggat tactggcgga atcggcaatg accagcaact cactcggccc cgcaatcata
     9181 tcaatgtcga cttggccata gactagacgt tttgccgtgg ccacatacaa attccctgga
     9241 cccacaattt tatccacggg ccggattgtc gaggtaccaa aggccaaggc cccaatggct
     9301 tgggctccac cgacccgata gatttcatcc actcctgcca catccgccgc caccaataag
     9361 gcgggatgga taatgccggt cggggtcggc gtacacatga cgactcgctt cacaccagcc
     9421 actttcgcgg gaatggcatt catcagcacc gatgatggat acagtgcttt tccgcctgga
     9481 acatacacac cgacggcatc taacgacgtg atccgttgcc ctaattgaat tccctcttta
     9541 cgatagaccc aggatgccct tcgttgtcga cgatgaaagg tccgaatacg attcgcagca
     9601 tatttcaagg ctttagtatc tgaagacgga agttcggcat acgcacgttc gacgtctttc
     9661 ggcgtcacac gaagaccgtc aggactaatt cggacgtgat cgaatttgta ggtcaatcgc
     9721 tggagggcct ggtcgccctc ttgccgaaca gcctccaaaa ttgtcctgac gactttttca
     9781 actttggtat tttgttgagc tccccgctga catacgcgat ccaccgcctg cgaaaaccct
     9841 tggtccttgt gggaaacaat tttcatacgt ggaccttttt ggagcgagaa cgggaaagaa
     9901 ttttcttcac ccgagcaatc agttctgtaa tcacggcatg tttcattttc aagctcgccc
     9961 ggttcacaat caatctggcg gatgaccagg cgatgacctc ttcctctacc agattattgg
    10021 ctttcaaggt gcttccggtc tccacaaggt ccacaatccg atcagccaac ccgaccaatg
    10081 gggccaactc gatcgaccca tagagcttaa tcaattccac aggcaaccct tgttggttaa
    10141 aataacgttc ggtgatcgtg ggatatttgg tggctacccg aagcttggaa gacatccgtt
    10201 gtccaagctg tggcccgttc ggcctggcga ccaccagccg acagacaccc aagttcaagt
    10261 caaccggttc atagacatcc gggctctgct ccaacaagac atcttttcca accactccta
    10321 aatcggcggc gccatattca acataagtcg gcacatcgct ggggcgaaca attaagaccc
    10381 ggtgcccgtg ctcagagaat tccaaaatta atcgacggtc atccgctcgc acatgatatc
    10441 ccgtatagcc tgcctgccgg aacaattcaa gagtcggatc aagaagttta cccttggaaa
    10501 gggcaatggt aatcacgtca ctcatgccac ctcgcgaatc cgctcaatct tcgcacccag
    10561 ggcctgaaat ttttcttcca gccgctcata gccacggtct aaatgataga ctcgagacac
    10621 agtcgtttcg ccatcagcgg ccaatccagc caacaccaaa cctgcgctgg cgcgcaaatc
    10681 cgaggccatg acgggtgcac cggtgagcaa cggcacacct ttaaccacgg catgtggccc
    10741 atcaatctca atcgacgcgc ccatccggtg aagttccggg acatgcagaa atcgcccttc
    10801 aaaaatggtt tcagaaatca cgctcactcc ctctgccacc gacatcaacg ccatcatttg
    10861 ggcctggaga tcggtcggga agccgggata ggggagagtt ttcatctcca ccgctttgag
    10921 cggaccggaa cgacagattc gtacgccgtc atggcattcg gttaattcag cacccatttc
    10981 ctgtattttt ttcaggacgc tgcctaagtg atccgtcata caccccttca ccatgacatt
    11041 acctccggta atggctccgg ccatcaaata ggtgccggct tcaactcgat cggggatcac
    11101 ccaatgccct gccccatgaa gttcttttac cccttcaatc gtaatggtgt ccgtgcccgt
    11161 cccatgaatt ttggcgcccc gcttggtaag gaatgcgcag agatccgtga tttccggttc
    11221 ccgcgcaaca ttatgcaaac tcgtggtccc ttccgccaga caagccgcca tgatcaggtt
    11281 ttccgtgccc gtgacagtcg ggaaatccag gtcaattctt gcccctttga gacgacaggc
    11341 tttcgcatgg atatatccat gctccacctg aatttcagcc cccatcatct ctaatccttt
    11401 aaggtgaaga ttgaccggtc tggtgccgat cgcacatccg ccaggcagag aaaccttggc
    11461 ttctcccagc cgcgcgagta aagggcctag ggctaaaatc gaggctcgca tcgttttgac
    11521 caaatcatac ggcgcttcaa tcgattgcac ctgagccgca tccagtctga ttctgtcgcc
    11581 ttcctcttga accgtcactc ccaacaaggc cagtaatttc cccatcgtca tcacatcacg
    11641 aacgcgtgga acatggtcga tcacgcattc cccaccccct aataacgtcg ctgctaaaat
    11701 gggcaaggcg gcattcttgg ctccgccgat ttcgacggcc ccctttaatg gcaatcctcc
    11761 cgtgattcga atttgatcca tttacaatat tccgtgtcta cacaagacgc tgttgcacgc
    11821 acaccactcg tggaatgcct tgggaatccg gccggacctt taccgtctta aacgcattcg
    11881 tgagtgaagg atcccggcac agaagatcag cctggccgac tcccacttcc atgaggataa
    11941 atccctttga ggagacaagt cccggcgatt catccaataa ccgccggtac acatcgagcc
    12001 catccggccc tccatccaac gccagccgtg gttcaaaatc tttgacatcc ggagaaaggt
    12061 gttcccattc cgcatgggag atatacggca ggttagcaat aatggcggtc actttacctg
    12121 ctaaatttct ggataacaag tgggaaagga ggtcaccggc cacccacagg attcgttgcg
    12181 aaaccccatg gcgcgcggca tttatctgcg caagatgcag cgccgtgact gacctgtctg
    12241 atgcgataat cattgcgcca ggaaggtgga tcgccagact gatcgccaaa catccggaac
    12301 ccgtcccgac atcaagaatc accggccgcg cgtcaccacc cagaagccca agagcctcct
    12361 ccaccagcaa ttctgtctcc ggtcttggaa tgagcacccc tggttgtacg agaaaatcca
    12421 acccacaaaa atcttgggtt cccaatacat attgaagtgg ctcatgtaac gcacggcgct
    12481 gaaataaggc gacagcccgc tgacagacat ccgcctctac gggagtatct ggattcgcat
    12541 gcagttgcag tcgcgtaatg ccaagggcat cctcaagaat ccacagggct tcatggcggg
    12601 cattggcaat ccccgcttga gtcagcgtct caaccgcacc acgatataac tgggagtagg
    12661 aagtgaaggt gggcatctga gcctcaaaaa gcgcacatgc gttgaacagg tgaaggagag
    12721 ggatcgggtg gttacacgaa aggcatcatt gggctttgag gcccgcgatt aacccattaa
    12781 gatcgccttc cattacccga tccaatttgt gcaacgtcag cccaattcga tggtcggtca
    12841 cgcgattttg tggaaaatta tacgtgcgga ttttctcact ccgctcccct gtcccgactt
    12901 gggaacggcg ttgttccgca atggaggctt cttgtttttc tcgctcggca tcgccaattc
    12961 gcgtccgaag ggttctcatg gcctttgtac gattttttag ctgtgatcgc tcgtcctgac
    13021 aagttaccac gacccccgtc gggatatgag tgatccggat cgcggaatag gtcgtattga
    13081 cactctgtcc accagcccct gatgaacagt aggtgtcaat ccgtaaatcc ttcggatcaa
    13141 tgtgcacttc cacttcttca acctctggca tcaccgctac cgtggccgtg gacgtatgaa
    13201 ttcgtccatt cgcctctgtc gtgggaaccc gctgcactcg atgcacccca ctttcatact
    13261 taaaattccc aaaggctccc ttgccttcaa ccagcaatac cgcctctttg acccctccaa
    13321 gacccgtttc ctgaaattcc atcaattcaa ctcgtaaccc ctttgcttcg gcaaacttcg
    13381 tgtacatacg aagaagatcg ccggcaaaaa gtgccgcctc actcccccca gttccagcgc
    13441 gaatttcaat gaacgaattt ttattatccc cttcatccgc cgggcaaagt aactcaatga
    13501 cctgagcctc catggcttcg cgttgacttt ccagactctg caattcttcg cccgccaagc
    13561 gacgcatgtc gggatccaat tgcagatctt ccgtcatcag ccgagcctga gcaatctcct
    13621 cgacaacccg tctgtatcca ccgaataact gcgcaatggg ctcaagctcc gcccgctctt
    13681 tattgagcgc aaccagaagg gtcgattgag acaggatagc cggatccgat aactgatcgt
    13741 tgagggtgtc atacttattc gcaatggcct cccatttgct aaataatccg ccctctttca
    13801 gctctgcttt agtaactgct gacatatacg tagcttaaca ggaaatcaga ttacccaaca
    13861 ggtctctttc ctgaacaacc gtgaccgcca tcctcatgcg aacattttcc tgtcaatggt
    13921 gggattatat cgaggaaccc ttgtcagcat tttggtgcac ccatgctgaa ataccccgtc
    13981 ttgtcgaccg cagatggaga aataagggca gacagggctc cttcaatacc ctgcacggac
    14041 gttatcccct ccaggattgt aaaggcagga caatggagaa gaggaaaaag gctttatgct
    14101 ttggctttgg gcttcgtcaa tttggcgtat tttttattga aacgttctac ccgtccttca
    14161 gcatccacaa ttttttgggt gcccgtgaaa aacggatgac aactggaaca gatatcgacc
    14221 ttcaagtctc ccacggtcgt ccgagtttga taggtcatac cacatgcaca actcacattt
    14281 gcaaattcat aagccggatg aatgcccttt ttcatactac ctccttcaac gccatccctc
    14341 gattcaaaat ctcaatcttc aaaagcctca acacgaggcc cctattgtga attatcgatt
    14401 catagattgc aagaactcat tattgctctt ggttcccccg attttgtcca tgagaaactc
    14461 catcgcctcc atggtcccga gaggacttaa tacctttcta agaatccaca ttttgttcag
    14521 gcggtcgcga tcaaccagta actcttcttt cctcgtgccg gattgactga tatcgatggc
    14581 agggaagagc cgtttatccg ccaaacgacg atccaaatgc acttccatat tgcccgtgcc
    14641 cttaaactct tcaaaaatga catcgtccat tcgacttccg gtatcaacca atgctgtggc
    14701 cagaatcgtc aaacttccac caaattcgat atttctggcg gcaccaaaaa accgtttagg
    14761 ccgttgaagg gcattggaat ccaaaccacc tgataacacc tttccactgg gaggagaaat
    14821 cgtattgtaa gcacgtgcca atcgagtcac actatctaat agaatgacca catcccgttt
    14881 atgttcaacc aacctcttgg ctttttccat gacaatttct gccacttggg catggcgctg
    14941 gggcggctca tcaaacgtgg agctaatcac ttcggcggcc ttgacctggc gctgccagtc
    15001 agtgacttct tcaggtcgtt catcgatcag gagaacaatt aaaacaattt ccggatgatt
    15061 cgcaataatg gaacgcgcca cagcctgtaa gagcatggtc tttcccgtcc ggggagccgc
    15121 cacgattaat cctcgttgtc ctttgccaat tggcgtcgtg agctccatca ctctggtgca
    15181 atattcttca ggatcaaatt ccaggttaat ccgttcttca ggatagagcg gagtgagatt
    15241 atcgaacaaa attttatccc gctgcacctc aggctcgtca taattaattt tttcgacttt
    15301 caagagcgca aaataccgtt ctccgtcctt cggcggacga atctggcctg agacaatatc
    15361 acccgttcgc aaattaaatc gtcgaa
//
LOCUS       DAORAQ010000060        15069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_667_length_15069_cov_69.020514, whole genome shotgun sequence.
ACCESSION   DAORAQ010000060 DAORAQ010000000
VERSION     DAORAQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15069
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_15069_cov_69.020514"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKM72_07355"
     CDS             <1..209
                     /locus_tag="PKM72_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60638.1"
                     /translation="ADLPHIFERFYQGKTQEGRVYVGSGIGLALAKRVVEAHGGKIWA
                     ESILGTGTALHFTIPKRKHTGTVS"
     gene            317..1033
                     /locus_tag="PKM72_07360"
     CDS             317..1033
                     /locus_tag="PKM72_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60639.1"
                     /translation="MAQSVSPGYSTGPSVLTPDPEDVPFFRAIADDQEKKLGSCKNEA
                     ECRAVHFLLGVAALYENRELAALHFRKVVVSNPNSALAGESRFWLWLLDVLNAPGGGT
                     LPEGLTKRLVREIVNKELLVHELSRQLETDSVDALKQELTNREKAVEELNQALSNLSK
                     QTEQLKKEQTLRQDVQLELKASERKVQELTNQLEALRRIDQEIREKAPPTRPSEKMAP
                     TPKLERAEEKEIPNEGEGKK"
     gene            1044..2435
                     /locus_tag="PKM72_07365"
     CDS             1044..2435
                     /locus_tag="PKM72_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP60640.1"
                     /translation="MADERILVVDDDPSLLTLLKMRLHSMGFVVTACGSGGDALSRVQ
                     EDPYDFAILDLRLPDADGLDLMEELHQHQTNLPILILTAHGCIPNAVEAMKKGAYGYL
                     TKPFDDKELLESIDKALTTKRMSQEIHRLQLLVNELYGLENVVARSAEMHTVLKQVAQ
                     VAKTDTTVCISGETGTGKEVIARVLHCNSPRASKPFMAVNCAAIPETLFESELFGHMK
                     GSFTGAHQNKRGLIQSADKGTLFLDEIGEMPLSLQGKLLRTIQNREVLPVGGQVPTKV
                     DVRILAATNKDLELAVREGRFREDLYYRIHVVPLWIPPLRQRREDIPSLAQFFLKRSI
                     EHHGKVVKGFSPEAMQALITYPWPGNVRELENMIERAVVMSPQDIITAEFLALSPHEQ
                     SKPGSLQPLTDAKEAFEKEYLQDLLKATQGNISKAAQIAGRYRADFYKLLKKYGLHPS
                     DRQDDAPSSTGQP"
     gene            3216..5405
                     /locus_tag="PKM72_07370"
     CDS             3216..5405
                     /locus_tag="PKM72_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60641.1"
                     /translation="MSVWVIPSILTAGVSLFLATIIFGKRRDGAHFFPMLLLMVVMVW
                     IHGLNVIQEALPAHLIISRKLILIGEIILPVMIGYVGYSLLQEFSANSLALGKGWWKV
                     IAGGAALLAMLIIGIPDGFMQVNSEREVVFKRPEGLMVWGFIVLASCVAIFQLERMLK
                     SFPDPLQYRLKYVLIGLGGLACISIAQAFHVAVASVWKQEDVWAGEIASFISLVVMAL
                     GLRRWPGPQVIQKIQISHHAVYASLAVVCVSGYFVLVAMVIALVQKTGWEVKESLGVI
                     LSFLAIMGLVIAVLSRRIRVEFQQFISRHFLKDKYDYREKWIEVTETFAACPDVQQIL
                     NRYLECLSRTFGTSRVTIWKYFDVDGRYHQIRTERRRRKLTENGWSPNISEPIPIPET
                     HPLIQQLKAQQEPLFVQAGQPAAEDSREFLDFTHAHVCVPLVTGQGRLLGFCTLSQES
                     AYECYDQDDFDLLRAIAHHVTMLLIQFELLEERSSSAKWEAVHRFSAFYLHDLKNLAS
                     SLSLVAQNAAQYGSNVEFQTSAMQTVKNTSQRIIDLMGELSRQAKEPNLSEGTVTESV
                     DVNLLIQDTLAAVNDPGCQTNFSPGLEVPVLQLKKEAIKQVFLNLILNARQATDRNGT
                     LDISTAYDGEQVIVELVDSGGGIPVAQLEKIFQPFKSSKKNGLGVGLFQCKRIIEDHH
                     GVIHIESQEGRGTTVIVTFPVQSADNASTVVFGVSNVGVKSDSGRNE"
     gene            5402..6787
                     /gene="prsR"
                     /locus_tag="PKM72_07375"
     CDS             5402..6787
                     /gene="prsR"
                     /locus_tag="PKM72_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNP60642.1"
                     /translation="MTMKSDPLAESSRLPSLLLVDDHPEILEQMKWGLKSSYMIYEAD
                     QRVAAVDILQREKISLVTLDLGLPPDTGGVTEGLAALEQLLAVNPLVKVVVITGNQDR
                     ANALKAIQLGAYDFLEKPVDLEVLKVVLQRAGYLAGLEKENQKLLDREAKRGFPEIIG
                     SSPVMVKVFDTIRRVAGSDISILIVGESGTGKELVARAIHQQSHRKDGPFIAINCGAI
                     PETLLESELFGHEKGAFTGAHLQRPGRIESSQGGTLFLDEIGEISPALQVKLLRVLQE
                     RSIERVGGRVEIPVDTRVLAATNMDLQLAMKDGRFREDLYYRLNTVTITAPPLRERGG
                     DIVMLGKSLLQRYSEEAKKKISGFTREALLSLEQYHWPGNIRELENRIRRAVTLTDNP
                     RIAPEDLDLPEPDESQVGLTLKVARDTVEKRVIEQTLAKTGGNITKAATILAVSRPTL
                     HDLISRHHIKK"
     gene            complement(6842..8050)
                     /gene="argJ"
                     /locus_tag="PKM72_07380"
     CDS             complement(6842..8050)
                     /gene="argJ"
                     /locus_tag="PKM72_07380"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNP60643.1"
                     /translation="MSKRVKGGITAPQGFVAAGMHSGIKKTKQLDLTMIMSEKPGPIA
                     GVFTSNRLPAAPVILDRLYLKRGMGQAFIINSGNANAFTGQEGLAHAREMGQRVADQL
                     TIPLHHVFVGSTGVIGVPLPMSAIRSGIPILISRLRKAGHREAAKAIMTTDTCPKEIA
                     IQDRIGGKTVTIGGIAKGSGMIHPDMATMLAYITTDAAIDQKTLQATFRAVTKQTFNC
                     ISVDGETSTNDTALCLANGKAGNLTIKTGTTAHGKFEALLFQVCHHLAMEIVRDGEGA
                     TKIIEFQITQASSDRAAKQFANTLATSPLVKTAIFGTDPNWGRIVAALGRADSPVKAE
                     KLLIEFNKIPIVQNGKGLGPQVERRIKQEMKKPFLTISISLGMGKGSSKIWTTDLTYD
                     YVKINASYRS"
     gene            complement(8094..9170)
                     /gene="argC"
                     /locus_tag="PKM72_07385"
     CDS             complement(8094..9170)
                     /gene="argC"
                     /locus_tag="PKM72_07385"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNP60644.1"
                     /translation="MNKKIRVAVVGASGYTGGELLRLLTLHPQVTLNRVVASEKSEGI
                     AVASLLPHLTKIFDCSLSSLNTEAIAKEVDVVFLALPHTQSLQPVADFLSLGKHVIDL
                     SADYRLQDPTVYEQWYQTPHTFPDLLKNAVYGLPELNRAAISKTHLVAVAGCYPTVAI
                     LQLAPFVAQNLIEQNSIIIDAKSGISGAGRTPALGTHFPESHEAIHAYKIGKHRHVPE
                     IEQGLARLAPAPSSKTTSSSPSIIFTPHLIPINRGILSTAYAKLKPGIDQSHLDAAYK
                     SRYQDEYFIRLFPSSEGVNPKNLRGSNFCDLSCTYDPRTGYLVSTGSLDNLVKGAAGQ
                     AIQCMNLMLGFPETLGLTAPGLFP"
     gene            complement(9361..9753)
                     /gene="rpsI"
                     /locus_tag="PKM72_07390"
     CDS             complement(9361..9753)
                     /gene="rpsI"
                     /locus_tag="PKM72_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNP60645.1"
                     /translation="MVDTVQYATGKKKNAIARAWVEPGQGDILINARSVDSYFPRLTH
                     QIQIQTPFEVTKTGGQFNVKATLTGGGVSGQAGALRHAIAKALALYNPSLRDPLKKNG
                     LLTRDSRVKERKKYGQKGARARFQYSKR"
     gene            complement(9800..10228)
                     /gene="rplM"
                     /locus_tag="PKM72_07395"
     CDS             complement(9800..10228)
                     /gene="rplM"
                     /locus_tag="PKM72_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNP60646.1"
                     /translation="MRSYQAKPLEVERQWFLVDAKGKTVGRLAAKVASILRGKHKPTF
                     TPNVDTGDHVVIINSSEIQFTRDKFRSKIYYHHTGYPGGIKAITAEHLHAKKPTEVLS
                     KAIRGMLPKCTLGKQMARKLKIYPGAEHLHRAQSPTPLNL"
     gene            complement(10266..10736)
                     /locus_tag="PKM72_07400"
     CDS             complement(10266..10736)
                     /locus_tag="PKM72_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNP60647.1"
                     /translation="MTEINTLIPLKVHGVLVDPNTDTQIVVLRDEKNSEVLPIWVGTA
                     EGTSIRLALENVIPPRPMSHDLICSFASHLGFTLNKVVITEVKNNTYFSTLFLSKDDL
                     EKSIDSRPSDAIALALRCQCPIYVTPEVLERRGGEDLDTWLSKLDQKGLEQTDI"
     gene            complement(10792..11247)
                     /locus_tag="PKM72_07405"
     CDS             complement(10792..11247)
                     /locus_tag="PKM72_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNP60648.1"
                     /translation="MLNQMNVRGLLFDPYNNAYIVILRDEQNSDMLPIWVGKAEASAI
                     SFALERIAPPRPMTHDLMKTVLDNMDAKVISTVITDLKDNTYFAKIHLLFGDSEFTVD
                     SRPSDAIAVALRTDAPIFASGEVLHKQNSEELERWLENLKPEDFGKSDV"
     gene            complement(11483..13204)
                     /locus_tag="PKM72_07410"
     CDS             complement(11483..13204)
                     /locus_tag="PKM72_07410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNP60649.1"
                     /translation="MRVSESLIPTMRQDPGEAEVVSHRLMLRAGMIRKVAAGIYSYLP
                     LGLRVIRKIEAIVREEMNRTGAQELLLPILSSAELWKETGRWDFYGKELFRLQDRHDR
                     DFCLGPTHEEVVTDLFRREVSSYRQLPLTLYQIQTKFRDEIRPRFGIMRGREFIMKDA
                     YSFDQDAEGAKRTYQGMYDAYCRIFSRMGLEFRPVEADTGLIGGISSHEFMVLANTGE
                     ELIVFDPDTKYAANVERAEIAPPTIGKRDAPLPSEKVSTPNAKSVEEVCALLNVPPSR
                     LVKTLLYQTGTDEITAVLIRGDHQANEIKIQRHLGIHELSLATPEMISRFTTAPIGFV
                     GPIGLQNVRILADHAVAVLSNFIVGGNEPDVHFIHVNIDRDFSIESIGDFRQAQAGDP
                     SPRGGSSLQTARGIEVGHIFLLGTKYSEKMGATYLDDQGQTLPAIMGCYGIGVSRAAA
                     AAIEQNHDDKGICWPMPIAPFHVHILSVTASDNVRQASNQLYQDLSKNGIEVLLDDRE
                     ERGGVKFNDADLLGIPFHIILGDKGLAQNTIEIKDRKTGEKHHISREEACEWITATVK
                     AKLEFQA"
     gene            complement(13292..14683)
                     /gene="rseP"
                     /locus_tag="PKM72_07415"
     CDS             complement(13292..14683)
                     /gene="rseP"
                     /locus_tag="PKM72_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNP60650.1"
                     /translation="MNNLLAFSPDSIFLFGQKLWWFLVVLGVLVTFHEYGHYLAARWV
                     GVKVLKFSIGFGPKLIGRKIGDTEYLLAAIPLGGYVKLYGEEGSEAVSAAEQRESFIH
                     QSLPHKTLIVAAGPGFNFILSYLIFTGMLALGSPLFVPSIDNITPVIEAIIPDSPAET
                     AGLHIGDRVIRANEEDISTLGELYEQVGKAHGRPVTLDVIRGNSVKTLIITPTVQTVP
                     DRPDEPQYTLGIEDHAPLVGGVMPDTPAMAAGLQQDDRIIQINDTPIATWSQMTEIVR
                     NHPGTPLEVQVERAGQTVSLRITPEGQTSTSPDGETTSIGRIGVKLAGAGTVLKSTSL
                     FLAPWDGLKATWKWCELTVMGLYKLVTGEISSKHLGGPLMIATVSGEQAQQGMASVVW
                     LIAILSINLGILNLLPIPILDGGHLFFFACEAILGRPLGDRSREMAQQIGLVLLVFLM
                     AYATWNDISRLLQ"
     gene            complement(14708..>15069)
                     /locus_tag="PKM72_07420"
     CDS             complement(14708..>15069)
                     /locus_tag="PKM72_07420"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNP60651.1"
                     /translation="ALKYPERIPLNPPLLDLGKIGKFTFYPTDSEKFPCLQLAYDALA
                     GADGLPATLNAANEIAVDAFLKEQIAFLDIPRVIQETMNAYNATPVTSIEDTLEIDQW
                     ARRTATEMMKACSTSPL"
BASE COUNT         3807 a         3267 c         3926 g         4069 t
ORIGIN      
        1 aagccgattt gcctcatatc tttgaacgat tttaccaggg gaaaacccaa gaaggtcgtg
       61 tgtatgtggg gagtgggatt gggctggctt tggcgaagcg tgtggtggaa gcccatggtg
      121 gaaaaatttg ggcggagagc attcttggaa ccgggacggc gttgcatttt accattccga
      181 agcgaaaaca tacggggact gtctcttgat acgcaaacag tttgagtcgt tgatctgggt
      241 gttttttggg tctctgataa ttctaatttc tcttgaatgg ggagagtgga attttaccgg
      301 ctggaagagt ttggctatgg ctcaatctgt ttcacccggg tattccactg ggccctcggt
      361 gttaactcct gaccccgaag atgttccatt ttttcgggca attgcggatg atcaggaaaa
      421 gaagctgggt tcatgtaaaa atgaagctga atgtcgggcg gttcattttc ttcttggagt
      481 cgctgcgtta tatgaaaata gggaattggc tgcgcttcat tttcgaaaag tggtggtctc
      541 aaatcccaat agcgccttgg cgggtgaaag tcgattctgg ttatggttat tggatgtatt
      601 gaatgctccg ggaggaggga ctttaccaga gggtcttaca aagcgattgg ttcgtgaaat
      661 tgtgaataaa gaattattgg ttcatgaatt aagtcgtcaa ttagaaacag actctgttga
      721 tgcacttaag caagaactga cgaatcggga aaaggctgtc gaagagttga atcaggctct
      781 ctcgaatctt tccaaacaaa cagagcaatt gaaaaaggag cagacgctcc ggcaggatgt
      841 acagctggaa cttaaggcca gtgagagaaa agttcaagaa ttgacgaatc aattagaggc
      901 tttacgccgt atcgatcagg aaatacgcga gaaggccccg ccgacccgcc cgtctgaaaa
      961 aatggcccca acccccaaac tcgaaagagc cgaggagaaa gaaatcccaa atgaaggtga
     1021 aggaaaaaaa tagaggaatt gtgatggctg atgaacgaat tcttgttgtt gatgacgatc
     1081 caagtttact gaccctcctg aaaatgcgcc tgcattccat ggggtttgtg gtgactgcgt
     1141 gtgggtcggg gggagatgcg ttgagccgcg tacaggagga tccctatgat tttgccattc
     1201 tggatcttcg attgccggat gctgatggct tggatctcat ggaagaattg caccagcatc
     1261 aaaccaatct tccaatcctg atcttgacag ctcatggctg tattcccaat gcagtggaag
     1321 cgatgaaaaa gggtgcgtat gggtatttga ccaaaccgtt tgatgacaaa gagttgctag
     1381 agagtattga taaagccctc actaccaaac gaatgagtca ggagattcat cggctacaac
     1441 tgttggtgaa cgaattgtat gggctcgaaa atgtcgtggc acggagtgca gaaatgcata
     1501 cggtgttgaa gcaggtggct caagtggcga agaccgatac caccgtttgt atttccggag
     1561 aaacagggac cggaaaggaa gtgattgcac gcgtgcttca ttgtaatagc ccccgagctt
     1621 caaagccgtt tatggccgtc aattgtgcgg caatcccaga aactttgttc gaaagtgagt
     1681 tgttcggaca catgaagggt tcctttaccg gagcccatca aaacaagcgg ggtttaatcc
     1741 aaagtgccga taaagggaca ttatttttag atgaaatagg tgaaatgccg ctgtcattgc
     1801 aagggaaact cctgcgaact attcaaaatc gggaagtgtt gcctgtcggt ggtcaagtgc
     1861 ctacaaaggt ggatgtgaga atcctggctg caacaaataa ggatctggaa ttagctgtgc
     1921 gggaagggcg atttcgagaa gatctgtatt atcgaattca cgtcgttccc ttgtggattc
     1981 ccccgcttcg acaacgtcgg gaggatattc cttctctcgc ccagtttttt cttaagcgga
     2041 gtattgagca tcatgggaaa gtcgtcaagg gattttcacc ggaagctatg caggccttaa
     2101 ttacctatcc gtggcctgga aatgttcgag aacttgaaaa tatgattgag cgcgcggttg
     2161 tcatgtcccc tcaggatatt attacagcag aatttctcgc cctttctccc cacgaacaaa
     2221 gcaagccagg tagtcttcaa ccattaactg atgcgaagga agcgtttgaa aaggagtacc
     2281 tccaggatct gctcaaggcc acacaaggga atatctccaa ggcagcacaa attgccggtc
     2341 gctatcgggc ggatttttat aaactgctca aaaaatatgg cctgcatcct tccgatagac
     2401 aggatgatgc gccttcttca accggccaac catgacccca gtaatttggg taccgctgtc
     2461 aagaagctta ggtttcgcca tggggtcggg aaggcgccag gttcgcttca ctttaaaaac
     2521 ggcagttaag tagtctcacg ctgatttata cttccagatt ttcggcagaa atcggctgaa
     2581 aaggcctata tatacctggt atgtttcatt ccagcaattc ggataaggcg ttcctgtcga
     2641 atagccgcat gtcccccttt ctgcatgact caatttattg gctgaaacgg gacggctctg
     2701 tcctgtagat ggagaatcta aaaatactcc tattcatcag aatcgaaccc tagctttcgt
     2761 tgtcaggcga gaggcgaggt cgatttgaga agccgaaccc tacagtcccc ctcctcatct
     2821 tccgaaaaaa ctactacagt tttattctaa ctaatgtcct gtgaggcagg ctagagtggt
     2881 tctctatctt gagctttctc tccatgagag aggtgtcata gaaacctgtt gggcgaaaat
     2941 cctttatcta ttaaaacaag ctttagtagc ttgatgactg agtcctcatg agcctggccg
     3001 gaagaaataa ttttgccttg atacgcaaag gactcgatga ccagcgtagg tagtcgggtg
     3061 tttattccat aaacccaggt gcgccagagt gattgaggca tgtgctacgt agcatgtccc
     3121 catcaatcag aaaaatagaa aggctgtgtc ctttggtagc gccgagaaat gaacttgtgt
     3181 ctgtctctaa ctctcaatag gtattaaggg tcggtatgag tgtgtgggtt attccatcta
     3241 ttctcacggc aggagtctcc ctgtttttgg cgacgatcat atttgggaag agaagggatg
     3301 gtgctcattt ttttcctatg ctcttgctga tggtggttat ggtttggatc catgggctga
     3361 atgtcattca agaagcgttg ccagcccatc tcattatctc caggaaatta attctaattg
     3421 gtgaaataat tctgccggtt atgatcggct atgtcgggta ttcattatta caggaatttt
     3481 ctgctaattc cttggcattg ggcaaagggt ggtggaaggt catcgccggt ggggctgctc
     3541 tcttagcaat gttgatcata ggtattccag atggattcat gcaggtcaat tcagaaaggg
     3601 aagttgtttt caaaagacca gagggtttga tggtctgggg gtttattgta ttggcctcgt
     3661 gtgtggcaat atttcagttg gaacgtatgc tcaaatcttt tccagatccc ctgcaatatc
     3721 gattgaaata cgtgttaatt ggattgggag gtctggcttg tatatccatc gcacaggctt
     3781 ttcatgtggc cgtggcttcg gtgtggaaac aggaagatgt gtgggcgggg gaaatcgcat
     3841 cgttcatttc tctggttgtc atggcactgg ggttaaggcg atggccggga ccccaggtga
     3901 tccagaaaat tcaaatcagt caccatgcag tatacgcctc actggccgtt gtgtgtgtca
     3961 gcgggtattt cgttcttgtg gctatggtga tcgcactggt gcaaaaaacc ggttgggagg
     4021 tcaaggaatc actgggtgtg atccttagtt ttcttgccat tatggggttg gtgatcgccg
     4081 tgctttccag gcgtattcgt gtcgagtttc agcaattcat ttctcgtcat tttttaaaag
     4141 ataaatatga ttatcgagaa aaatggatag aggttaccga gacgtttgcg gcttgtccgg
     4201 atgtccaaca aattttgaat cggtaccttg agtgtctcag tcgaacattt ggaacctctc
     4261 gagtcacaat ttggaaatat tttgatgttg acggccgata tcatcaaata cgaacggaac
     4321 ggcgtcgaag aaagttgacg gagaatggtt ggtcaccgaa tatttcagaa ccgattccca
     4381 ttcctgaaac tcaccctctt attcaacagt tgaaagcaca gcaagaaccc ttgtttgtac
     4441 aggcgggtca gccggcggcc gaggattcga gagaatttct tgatttcact cacgcccatg
     4501 tgtgtgtgcc tttggtgact ggacagggca gactgctcgg gttttgtacg cttagtcagg
     4561 aatcggcata tgaatgctat gatcaggacg attttgattt actgagagcc attgcgcacc
     4621 atgtgacaat gctgctcatt caatttgagt tgcttgaaga gcgcagttca tccgccaaat
     4681 gggaagcggt gcatcggttc tctgcatttt atttacatga tttaaaaaat ttggcctcga
     4741 gtttatctct tgtggctcag aatgctgctc aatacgggag taatgttgaa tttcagacat
     4801 ctgcaatgca gaccgttaaa aatacgtctc agcgaattat tgatttaatg ggggaattgt
     4861 ctcgtcaggc gaaggaacca aatctgagtg aaggaaccgt tacggaatca gtggatgtga
     4921 atctgttaat tcaggataca ctggctgccg taaatgaccc tggatgccaa acgaattttt
     4981 ccccagggct agaggttccc gtccttcagc tcaaaaagga ggcaatcaaa caggttttct
     5041 taaatttgat ccttaatgcg cgtcaggcga ccgaccggaa tggtacgctt gatatttcta
     5101 ccgcctatga tggtgagcag gtgatcgttg aactggtcga ttcggggggt gggatacctg
     5161 tagcccagct tgaaaaaatt ttccagccat ttaaaagttc caaaaaaaat ggattgggtg
     5221 tggggctgtt tcaatgtaag cgaattatcg aggaccatca tggcgtgatt cacattgaga
     5281 gccaggaagg gcgaggtacg acagtaattg ttacatttcc ggtacaaagt gccgataacg
     5341 caagtactgt ggtgtttggt gtttctaatg tcggggtaaa gtctgactca ggaagaaacg
     5401 aatgaccatg aagtctgatc ctcttgccga atcttcacgt cttccttccc tgcttttggt
     5461 tgatgaccac ccagagattc ttgaacagat gaagtgggga ttgaagtcct cttatatgat
     5521 ctatgaggca gatcaacgtg tggcggctgt cgatattctt cagcgggaaa aaatctcact
     5581 ggtaaccttg gatttaggtt tgccacctga taccggcggg gtgaccgaag gattggctgc
     5641 cttagaacaa ttgcttgcgg taaacccttt ggtgaaggtg gtcgtcatca cgggaaatca
     5701 agatcgcgcc aatgcgctca aggcgattca attgggcgca tatgatttct tagaaaagcc
     5761 ggtagatttg gaggtgttaa aagtcgttct tcagcgggcc gggtatttgg ctggcctgga
     5821 aaaagaaaat caaaagcttc tggaccgtga ggcgaaacga ggatttccag aaattattgg
     5881 aagcagcccg gttatggtta aagtgttcga tactatccgg cgagtggccg ggtcggatat
     5941 ttctattctt attgttgggg aaagtggcac ggggaaggag ttagtggctc gggcgattca
     6001 tcagcaaagt cacagaaagg atggcccgtt tatcgcgatt aattgtgggg ctattcccga
     6061 aacgttgcta gaaagtgagt tgtttgggca tgagaaaggt gcgtttaccg gggctcatct
     6121 ccagcgtcca ggtcgaattg agtcgtctca gggcgggacg ctttttctcg atgagattgg
     6181 tgagatttct cctgcattac aggtcaaact gcttcgggtc ttacaagaac gaagcatcga
     6241 gcgggtaggc ggacgcgtag aaattccagt tgacacgaga gttttagcgg ccaccaatat
     6301 ggatcttcaa ctggcgatga aagatggccg tttccgggaa gatttgtatt atcgattgaa
     6361 tactgtgaca attacggcgc ctcccttacg cgagcggggt ggcgatatcg tgatgttggg
     6421 caaatcgttg cttcagcgtt attcagaaga ggccaaaaag aagatttcag gatttacgag
     6481 ggaagcctta ttgtcgctag aacagtatca ttggcctggc aacattcgag aattagaaaa
     6541 tcggattcgt cgtgccgtga cactaaccga caatccaaga attgccccgg aagatttgga
     6601 tttacctgaa cccgatgaga gtcaggttgg actcaccttg aaggtagcca gagacacggt
     6661 agaaaaacgg gtgattgagc aaactctggc caaaactggt ggaaatatta cgaaggctgc
     6721 gactattctt gctgtgagtc gtccgaccct gcatgattta atatcccgtc accacataaa
     6781 aaaataatcc tccctcggta atgggctatt ctcaattttc agattgaatg ctggatggaa
     6841 tttacgatcg ataggatgcg ttgattttca cgtaatcgta ggtcaaatcc gtggtccaaa
     6901 tcttggagga gccctttccc attccgagtg aaatggaaat cgtcaggaag ggcttcttca
     6961 tttcctgttt aattcgtcgt tcgacctgag gacccagccc tttcccattt tggacgatgg
     7021 gaatcttatt gaattcgata aggagtttct ccgctttaac aggggaatct gcccgtccca
     7081 atgcggccac aattctcccc cagttgggat ccgtgccaaa aatagcggtt ttgactaaag
     7141 gggaggtggc tagagtattg gcaaattgtt tcgcggcccg gtcactggaa gcctgggtaa
     7201 tctgaaattc tatgattttg gtggcgcctt cgccatcccg aacaatttcc atggccagat
     7261 ggtggcagac ttgaaataag agtgcttcaa atttgccatg tgccgtcgtt ccggttttga
     7321 tggttaaatt tccggctttc ccgttagcca gacataatgc cgtgtcattg gtgctggttt
     7381 ctccgtcaac agaaatgcag ttgaatgttt gtttggtgac ggcgcgaaag gttgcttgta
     7441 gcgttttttg gtcaatggcc gcatctgtcg tgatataggc caacatggtg gccatgtcag
     7501 ggtggatcat tcctgaacct ttggcgatcc cgccaatggt tactgttttt ccaccaatac
     7561 ggtcctgaat cgcgatttcc ttaggacagg tgtctgtcgt catgatggct ttggcagctt
     7621 cacggtgacc agccttgcgt agacgtgaaa tcagaatcgg aatgccgcta cgtatggcgg
     7681 acatgggtag aggaacccca atgacaccag tggatccgac aaacacgtgg tggaggggaa
     7741 ttgtgagttg gtcggcaact ctctgaccca tttcccttgc atgtgccaat ccttcctgtc
     7801 cggtaaatgc gttggcattg ccactattaa tgatgaacgc ttgacccatg cccctcttga
     7861 gatagagccg gtcgagaatc accggagcgg cagggagcct gttagaggtg aacaccccgg
     7921 cgatgggtcc aggcttctct gacatgatca tggtcagatc aagttgtttg gtctttttaa
     7981 tgcctgaatg catgcctgct gctacgaaac cttggggggc agtgatccca ccttttacgc
     8041 gtttagacat ggagacatcc tcttctcgtc aggagaaacc ggtgaaaaat ggtttaggga
     8101 aagaggcctg gcgcggtcaa tccgagtgtt tcgggaaagc ctaacataag attcatacat
     8161 tgaatcgcct gtccggctgc gccttttacg agattgtcca gagaacctgt gctgaccaga
     8221 taccccgttc gagggtcata ggtgcaacta aggtcacaaa aattcgaccc tcgcaaattt
     8281 tttggattaa ctccctcgga actgggaaag agtcgaatga aatattcgtc ttgataccga
     8341 cttttatagg cggcatccaa atgggactga tcaatgccag gtttcaattt ggcataggcc
     8401 gtgctcagaa tgcctcgatt aatgggtatg aggtgaggcg tgaaaattat ggatggactg
     8461 gaggaagttg ttttggagga tggagcagga gccagtcgtg ctaacccctg ttcgatttca
     8521 ggcacatggc ggtgtttgcc gattttatag gcatgaatgg cttcatggga ttcagggaaa
     8581 tgggtcccga gtgcaggtgt gcgacccgct cctgaaattc cagactttgc gtcaataata
     8641 atggaatttt gctcaatgag gttttgggct acaaatggcg cgagttgcaa aatggcgacg
     8701 gtgggataac acccggcgac tgcgacaaga tgagtcttgg aaatagccgc gcgattgagt
     8761 tctggtagtc catatactgc atttttgagc aagtcaggaa aagtatgggg ggtctgatac
     8821 cattgttcat agaccgtcgg gtcttgaaga cgataatcgg cactcaagtc aataacatgt
     8881 ttccctagtg acagaaaatc ggctactggt tgcagtgatt gtgtgtgggg aagagcgaga
     8941 aacaccacat cgacttcctt ggcgatcgct tcggtattca acgatgacaa tgaacagtca
     9001 aaaatcttcg taagatgagg aagcagcgaa gcgacagcga tgccttcaga tttttcagac
     9061 gccaccacgc ggttcagtgt gacctgtggg tgcagggtga gtaatcgaag taattcacct
     9121 cccgtatagc cactggctcc gaccactgcg acgcgaattt ttttattcat ggtcatttca
     9181 aagaacttgg tttggggaga aaaggacaat acctattagg aaatcatata actctgtgcc
     9241 aaatgcctgt ttgtggaaag gtcaattgag gtgggcaaag gcatcagaag atgacggatt
     9301 aaaaaagggg gaggctggat ggcctccccc atggtcctag gtgaacggtg agctggccaa
     9361 ttatcgtttg gagtattgga acctggccct ggcacctttt tgcccgtatt tttttcgctc
     9421 tttcaccctg ctatctctgg tgagcaatcc attttttttc aagggatccc gtaaggaagg
     9481 attatataaa gctaaggcct ttgcaatcgc atggcgaaga gctcctgcct gacctgaaac
     9541 gcctccaccc gtcaacgtgg ctttgacatt gaattgtcca ccggttttgg tgacctcgaa
     9601 tggtgtttga atttggattt gatgtgtcaa tcgtgggaaa tacgaatcta ctgatcgtgc
     9661 gttgatcaga atgtcgcctt gtcctggttc tacccatgct cgggcaatcg catttttctt
     9721 ttttcctgtt gcgtattgaa ctgtatcgac catgtgagaa tagatctcct tttttacggt
     9781 tgaacggtaa ctgatgactt tagaggttta aaggcgttgg gctttgcgct cgatgcagat
     9841 gctcggcacc agggtaaatt ttgagttttc tagccatttg ctttcctaac gtacattttg
     9901 gaagcatccc acgaatggct ttagaaagca cttcagttgg tttctttgca tgaagatgct
     9961 ccgcagtaat ggctttgatg cctcccggat acccggtgtg gtgatagtag atctttgaac
    10021 gaaatttatc gcgggtgaat tgaatttcac tggaatttat gatgacgaca tgatcccccg
    10081 tatcgacatt cggagtaaac gttggcttat gctttccacg aaggatagag gcaaccttcg
    10141 cggccaagcg tccaacggtt tttcctttag catcgactaa aaaccattgt cgttcaactt
    10201 ctagcggttt agcttgatag gatcgcatga gtgttgtatt tcctttcctc tacttgatta
    10261 tatgcctaga tatcagtctg ctctaatcct ttttgatcga gtttggaaag ccaggtatcc
    10321 aaatcttctc cgccgcgacg ttccaagact tcaggggtga cgtagattgg acattgacat
    10381 cgaagagcca aggcaatcgc atcactcggc cttgagtcga tcgatttttc caaatcatct
    10441 ttggataaaa atagcgtgga aaaataggtg ttatttttga cttctgtgat caccacttta
    10501 tttagggtaa agccaaggtg cgaggcaaag ctgcaaataa ggtcatggct catggggcgt
    10561 ggtgggatga cgttctccaa cgccaaccga atcgatgtcc cttcggcggt tccgacccat
    10621 atgggaagaa cctctgagtt tttttcatcc cgtaagacca cgatttgggt atcggtattc
    10681 ggatcgacca ggaccccatg gacctttaac ggaattaatg tgtttatttc agtcatcacc
    10741 cgcacactca ctattaatcg ttactatttc agatggcctc atgaacatca gctaaacatc
    10801 tgatttgcca aaatcctcag gcttgaggtt ttccagccat cgttccaatt cttccgagtt
    10861 ttgtttatgg agcacttctc ctgatgcgaa aatgggggca tccgtacgaa gagccacggc
    10921 aattgcgtca cttggccgag agtcaaccgt aaattccgag tctccaaaga ggagatggat
    10981 tttagcgaaa tatgtattgt ctttcagatc cgtgataacc gtactgataa ccttggcatc
    11041 catattatca agcaccgttt tcatgagatc atgggtcatt ggccttggag gagctatccg
    11101 ctccaaggca aagctaatcg cgctggcttc ggctttccca acccaaatag ggagcatatc
    11161 cgaattttgt tcatctctca ggatgacaat ataggcgtta ttataggggt caaataaaag
    11221 acccctgacg ttcatttgat tcaacattcg atatgccagt catagataaa aagtgatttt
    11281 cacttgaaaa tcaaacctag aaaagtagca ttcaatgaga tcttttgtca atgaaaataa
    11341 gggctttcca atctcatcat gctcttggtt gatatcccgt catttgtcct ccccagaaga
    11401 gaggatgagg gtttcaaaga attatatcat gcatggaagg acatcggtaa ccagcctgca
    11461 tttgatctgc cttccttgct gtttatgcct ggaattcgag tttcgctttg actgtagcgg
    11521 ttatccactc acatgcttcc tcacgggaaa tgtgatgctt ttcgccagtt ttacggtctt
    11581 tgatttcaat ggtgttctgt gccaggccct tatccccaag gataatatgg aacggaatgc
    11641 ctagcaaatc cgcatcgtta aatttaaccc cacctcgttc ctcacggtcg tcaagcaaca
    11701 cctctatccc gtttttggaa aggtcctggt agagctgatt ggaggcttga cgtacgttgt
    11761 ctgatgccgt gacggaaaga atgtggacat ggaagggggc aatgggcatg ggccaacaaa
    11821 tacctttgtc gtcatggttc tgttcaatgg ctgctgccgc tgctctgctg acaccaatgc
    11881 catagcatcc catgatagcg gggagcgtct gaccctggtc gtcaaggtag gttgcgccca
    11941 ttttttcact atattttgtc cctagcaaaa agatatgacc tacttcgatt ccccttgccg
    12001 tttgcagcga actgccccca cgcggggaag gatcacctgc ctgggcctgg cgaaaatctc
    12061 ctatgctctc aatggaaaaa tcccgatcga tgttgacatg gatgaagtgg acgtccggtt
    12121 cgttcccacc gacaatgaag ttgctcaaga cagccacggc atggtcagcc agaattcgaa
    12181 cgttctgaag accgatcggt ccgacaaagc cgattggggc tgttgtgaag cgggagatca
    12241 tttcaggggt cgccagggat aattcatgaa taccaagatg tcgttgaatt ttgatttcgt
    12301 ttgcttgatg gtcccctcga atcaggactg cggttatctc atccgtgcca gtctgataca
    12361 agagggtttt caccagacgg gaaggaggta cgttaagtag ggcacatacc tcttcaactg
    12421 atttggcatt gggtgtggaa actttttctg acggtagtgg ggcgtcacgc ttcccgatgg
    12481 tggggggagc aatctcagct cgctcgacat tggcagcata tttcgtatcc ggatcaaaaa
    12541 cgatgagttc ctctccggta ttggctagga ccatgaattc gtgagaagag atgcccccga
    12601 tcaagccggt atcggcctcc accggacgaa attcgaggcc catgcgagaa aaaatacggc
    12661 aataagcgtc gtacatgcct tggtaggtac gcttggctcc ttccgcatcc tgatcaaagc
    12721 tatacgcatc tttcatgatg aattcacgcc ctcgcataat gccaaaccgt ggccgaattt
    12781 catcacgaaa ctttgtttgg atttgataaa gggtaagggg caattgacga taggaactga
    12841 cctctcgacg gaataaatca gtgacaactt cttcgtgagt ggggcccagg caaaagtctc
    12901 gatcgtgacg atcttgtaat cggaatagtt cctttccgta gaaatcccag cgaccggttt
    12961 ctttccacag ctcggccgaa gaaagaatcg gaagtaaaag ttcttgagct cctgtccggt
    13021 tcatttcttc ccgaacaatg gcctcgattt tccgaatgac ccgcaatcca aggggaaggt
    13081 aggaataaat accggctgcg actttccgga tcattccggc gcgaagcatg agtcggtgac
    13141 tgaccacctc ggcttcaccc ggatcttgtc gcatcgttgg gattaaggat tctgatacac
    13201 gcatgaagac acctggttat atggttaagg aaaaaagccg cagggaagat tcatagggct
    13261 tgaaaggcag tagacgttaa acaggataga attattgcag aagtcgacta atgtcattcc
    13321 aggtcgcata ggccatcagg aagaccaaca agaccaggcc aatttgttgg gccatttctc
    13381 tcgatcgatc ccctaaaggg cgacctaaaa tagcttcgca ggcaaaaaag aatagatggc
    13441 ctccatcaag aatgggaatg ggtaacaaat ttaaaattcc caaattaata ctcaaaatcg
    13501 caataagcca cacgacgctg gccatgcctt gttgggcctg ctcgccggat acggtggcaa
    13561 tcatgagcgg gccgcccagg tgtttcgatg aaatttctcc agtcacgagc ttatagagcc
    13621 ccataacggt taattcacac catttccatg tcgccttgag gccatcccaa ggtgcgagga
    13681 agagagacgt actcttgagc accgtacccg cgccggctag tttgacgcca atgcggccaa
    13741 tagacgttgt ttctccatcc ggggaggtgc tggtttgccc ttcaggggtg atgcgaagtg
    13801 acaccgtctg ccctgcgcgt tcgacctgta cctcaagagg ggttccaggg tgattgcgta
    13861 cgatctcggt catctgagac caggtggcaa tcggcgtgtc attaatttgg ataattcggt
    13921 catcctgttg aagacccgca gccatagcag gcgtgtctgg catgactcct ccgactagtg
    13981 gagcatgatc ctcaatgcct aaggtgtatt gaggttcatc cgggcgatca ggcaccgttt
    14041 gaacggttgg agtgatgatc agcgttttca cgctatttcc acgaataaca tccaaggtga
    14101 cgggacgccc atgggctttg cccacctgtt cgtagagttc acccagggtc gagatgtctt
    14161 cttcgttagc ccgaattacg cgatcaccga tgtgtaatcc ggcagtttca gccggagagt
    14221 ccgggatgat cgcttcaatg accggggtga tgttgtcgat gctgggaaca aacagcggtg
    14281 accctaaggc cagcattccc gtaaagatca ggtagctgag gataaagttg aagcctgggc
    14341 cagccgccac aatcaacgtt ttgtgcggaa gcgactggtg aataaaggat tcacgttgtt
    14401 cagcagctga aacagcctct gacccttctt ctccatataa tttgacatat cccccaagag
    14461 gaatggctgc caggagatat tctgtatctc caatcttgcg accaatcaat ttgggtccaa
    14521 acccgatgga aaatttgagg accttcactc caacccagcg agcggccagg taatgtccgt
    14581 attcgtgaaa agttacaagg actcccagga cgaccaggaa ccaccataat ttttgcccaa
    14641 ataagaaaat ggaatcagga gaaaacgcca ggaggttatt catggatgtg taatcgtggg
    14701 ttgatattca tagaggtgaa gtggagcagg ctttcatcat ttccgtggcc gttcgtctgg
    14761 cccattggtc tatctctaag gtgtcttcaa tggatgtgac gggagtcgcg ttataggcat
    14821 tcatggtttc ttgaatcact ctgggtatat ccaaaaaggc aatctgctcc ttgaggaagg
    14881 catcgacggc aatttcattt gcggcgttta atgtggctgg aagcccgtcc gctcctgcca
    14941 gggcgtcgta ggctaactgg agacacggga atttttccga atcagttgga taaaacgtga
    15001 actttcctat tttccctaaa tccaggagtg gaggattcaa aggaatgcgt tccgggtatt
    15061 tgagagcat
//
LOCUS       DAORAQ010000061        14653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_695_length_14653_cov_65.175983, whole genome shotgun sequence.
ACCESSION   DAORAQ010000061 DAORAQ010000000
VERSION     DAORAQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14653
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_695_length_14653_cov_65.175983"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKM72_07425"
     CDS             complement(<1..237)
                     /locus_tag="PKM72_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60652.1"
                     /translation="MGTDPSSLQRLKIFMELIRQAKQQNAEPTFVSTPTEHGDNINVV
                     ATIEGKPATAGILFWDVSLLQNNGLADTLDEEDLA"
     gene            complement(342..557)
                     /locus_tag="PKM72_07430"
     CDS             complement(342..557)
                     /locus_tag="PKM72_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60653.1"
                     /translation="MGRKNSDDEIFPEMAAMLEGMQDLLCDEDGKPLSPDHPQYQKLT
                     GLMENIASRARKRDQQDPSTSGHQGTA"
     gene            complement(612..1247)
                     /locus_tag="PKM72_07435"
     CDS             complement(612..1247)
                     /locus_tag="PKM72_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNP60654.1"
                     /translation="MKSGQTQIARIIGGILLISLMGWANVALSTIQGEYELMEGEPSQ
                     HEAGKVILFEFADFYCSHCHMFERVVGTQLKKEFGDQLEIRMVGFPVIPGKLPTAFEM
                     YNQAVTMGKGPEMKELLFQAIHDKDIQVFDKTMRSLLLKEIGLDPTEFETGLASGKPF
                     KTLAKGRTWGERIKVTHTPTVLLDGNIRVANLTAENLKTVIESILKQDSKS"
     gene            1740..2060
                     /locus_tag="PKM72_07440"
     CDS             1740..2060
                     /locus_tag="PKM72_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNP60655.1"
                     /translation="MNVVNIQDYSQFSNEKMKKQNVFQSPRFFCDVYGFEPGQEQKGH
                     VHADQDKVYLVLEGQGTFRVGNESRILGEGQGTMAPAGEEHGVLNHTTTRLRVLVFMA
                     PNPG"
     gene            2318..2512
                     /locus_tag="PKM72_07445"
     CDS             2318..2512
                     /locus_tag="PKM72_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60656.1"
                     /translation="MTWSDATTQATFIMCAVFLMGTMMVGCSSAPEPQPDPSKKDIQQ
                     DSDRFFKKMSQEENQQTPSR"
     gene            complement(2637..4010)
                     /locus_tag="PKM72_07450"
     CDS             complement(2637..4010)
                     /locus_tag="PKM72_07450"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNP60657.1"
                     /translation="MINPVRVTLILALVVLGLGGYILLIDIPQSRQLEKQETQERQIL
                     PFDDRAITQITWATPADTIRLERDDQWRWMMTEPMRSPADAREIRRILRALTIGKIKR
                     YIEDGPSNLSAYGLEPPYLTLTLTTPTETQEIALGDAGPFAPSLYVQTKPDNQVVLTT
                     LDVMTFAQKSLTNFRLKDLLFFERDRVLELHIQLGSSTMVMTRMAGAHSLTPNWMFQS
                     PVKGPADKTAVGTLLMDLGGLAATGFIDTEEEKKRILEQPARIEATIQVVEGARTHHL
                     ELYQFADPEKAYAITSASGPLYEIPTGILKPITQGMFHFQDKRLFGMEVADIAMLTVH
                     TPQDHYVLINQHDEWILEDNPSAELNQEVVKLFVSRIVDTPAEIFHPDSSPASKDNGM
                     ASPNATISGMNRNGKEIGQLILGKRERGLVFAKGASLPGLFQVRSAILDQIPPKTKLI
                     RETNSTE"
     gene            complement(4012..5520)
                     /locus_tag="PKM72_07455"
     CDS             complement(4012..5520)
                     /locus_tag="PKM72_07455"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNP60658.1"
                     /translation="MASILGIAGTILALIGLGLPLFHPAGGSLSSILLGLGTLCLLGY
                     FAFHFRSLTALARTRSTRLGAHSLFSVLLAGVVVALLNFLAAKHAPEWDFSETQNFTL
                     SRQTYQVLRTLPREVKMTVFTHEGSPGYGGYQDLLTTYAKESPLITLTFIDPERQPDK
                     AKEYQVTRIDTVVVESGPQKVYLQRSSEADMTNALLRVTQDKKKRIAFVTGHGEKSLT
                     DTESSGLSRAGDALTKQGYEVGTVDWEDTASHEAAVLIVASSIEAVSAEDQHRIRQFL
                     EKGGRVLVMVDPASDASLDPLITPWGIQLGAGILADEQDRLGRGSPTALLVRTFTTHD
                     ITEDFTTPILFPVSRSVTFDTAQGKTWEYVALAQTSPESWEETDMNSPQPEFTAGQDP
                     KGPFTVAGLLIRKPVDQSPTAQSAVLIVGNAAFATNGYLTYPGNTDFFLKAIAWLAQE
                     DALVSLTPKDPAFHPFIPNPSQEQALVFFQVLFLPLLTLFIGLSVWKRRRSL"
     gene            complement(5526..6296)
                     /locus_tag="PKM72_07460"
     CDS             complement(5526..6296)
                     /locus_tag="PKM72_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP60659.1"
                     /translation="MTPVHTIVAKELRSAFVSPVVYVIAAIFLAIVGLLAYSAAANAS
                     NQAIRLMQIQNTYAQLNLNDMLFRPLFRSINLILMIILPLLTMRLFAEERKLRTFELL
                     LTSPIGVNEIVSGKFMSVIIVYSGLLSLTSLIPITLSAYATFDWRPIYLGYVALFLQG
                     ALLISIGLFASALTENQIIAAFLSFGCILGLWMLGALGGIVGDTTIGHILSYLSFSEH
                     YERLIRGLFNIKDILYYVSGIAFMWFAAHQAVDAYRWK"
     gene            complement(6323..7285)
                     /locus_tag="PKM72_07465"
     CDS             complement(6323..7285)
                     /locus_tag="PKM72_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP60660.1"
                     /translation="MIDVQHVTKRYGNATALDDISFSINKGEIVAFLGPNGAGKTTTM
                     RILTGFMPPTLGTVRIAGFDCLETPWEVKKHIGYLPETPPLYLELTVSEYLTFVGRIK
                     RLPDDQLTERMDTIITQTGLADVRGRVIGHLSRGYRQRVGLAQALLHNPPVLILDEPT
                     TGLDPNQIIEIRELIKSLAGSHTIILSTHLLAEATAICQRVIIIHHGRIVAVDTPEQL
                     GAKLRESETLSLTVKQSDPDLLEALHHIPGVIRVSQGATAHTYLLDTQMGRDIREELT
                     QFIVSRNVGLLELKTQPLTLEDAFKVLTQTNTPSAPNLSPGGPH"
     gene            7530..8159
                     /locus_tag="PKM72_07470"
     CDS             7530..8159
                     /locus_tag="PKM72_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNP60661.1"
                     /translation="MRKLFQGNYAVLGFLVALLSGCSSWHHVAIVSHGADLRSESSSS
                     DSSPPSLGAAPPASVPKPEPFLPLVSQPTKSENLPPEINSGVTAHSADDSLLPLKLED
                     VFFDYDQYSIEKEAVPTLKQNAQVLLKRYPSREVLIEGHCDERGTEEYNLVLGERRAM
                     AVKNYLVDLGITASSIRVLSLGKHEPFCLQPTLRCFRQNRRAHFVLKQP"
     gene            8347..8718
                     /locus_tag="PKM72_07475"
     CDS             8347..8718
                     /locus_tag="PKM72_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60662.1"
                     /translation="MRFWSLIIWFLLLFPSSALPEVWDFSCTEAVSLLRAAQDRVVRQ
                     HDQLQEAKFSLRHAPKEFDGCRRSRRGFQGGKIHCVTHQSPQGNLLRDILVAQRSLDA
                     SIQDFKKYQKDLGLSCSMPSP"
     gene            9082..9876
                     /locus_tag="PKM72_07480"
     CDS             9082..9876
                     /locus_tag="PKM72_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNP60663.1"
                     /translation="MLSLKKILASFFSPLSLCIEMIACGLLLLCFSRKQNAGKIFITL
                     GFILLVVSGYEGVSGRIIQTLESQYPPINLSQVMPPGGVANAQGSVKWIVVLASGIAG
                     DATLPYQLQVSYHSRVRLMEGIRLHRLLPGSKIILTGGTGFDGSPEATAMSRVAEELG
                     VSRADMVLEVESRDTKDHPHYVREIVHDEPFILVTSAFHMPRAVRLFEKQGLIPIPAS
                     TGHWVPPIQFWSLVNFFPSSTGVRLAELAYHEYLGLLWARFQGQIE"
     gene            10080..10403
                     /locus_tag="PKM72_07485"
     CDS             10080..10403
                     /locus_tag="PKM72_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60664.1"
                     /translation="MIGQKCGGGALILCLSFLSIGGCSIWESYSHPDHGPTRADRICH
                     PYGDCVQGVWVATDRAETGTAEAHAHCVTQVGAEQGNDWKKKSVTQGLEIGECMERRG
                     FLLKQ"
     gene            10710..10871
                     /locus_tag="PKM72_07490"
     CDS             10710..10871
                     /locus_tag="PKM72_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60665.1"
                     /translation="MARFVSVSPLAFVVGSMYEMLTTGLDPVTVILAEVRQVKAALIS
                     EVEIGGDMG"
     gene            11277..11858
                     /locus_tag="PKM72_07495"
     CDS             11277..11858
                     /locus_tag="PKM72_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNP60666.1"
                     /translation="MRKNQSIAPLSKGVKTVKSLKKKDSPRSLSKPKKILKALRLFAT
                     DVDGVLTDAGMYYGESGEELKKFHTRDGMGIKLLQAQGLITAIITMENTKIVARRGKK
                     LGIPEVFQGAKNKVAVLGHLSEKYKIPFAQMAYIGDDVNDVEALKTVGYAAAPADCVE
                     QVRQVVHYVCKKNGGEGAVREFIDTILAVKTPL"
     gene            11994..13442
                     /locus_tag="PKM72_07500"
     CDS             11994..13442
                     /locus_tag="PKM72_07500"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNP60667.1"
                     /translation="MNAHVYGVILAGGSGTRFWPLSRERFPKQFLRILGEGTLLQQTF
                     ERLLQKIPANRMAIVTNAVQAESIKLQLNQWKDDIAGNVILEPEGRNTAPAIALAALQ
                     LIHQDPEAVMVVVPADHVVKASKKFMQAVQFASELAVEGHLVTFGIQPTRPETGYGYI
                     QPLKRRKVGTKGDFIGYSVGRFVEKPNLTTARRYLQSGNYFWNSGIFVWKASQILSEI
                     AIHQPALSKLLSGIKRRMGSEEFPSYLRKVYAKVESLSIDNAVMEHSSRSVVVPIDFG
                     WSDVGSWSSLEEVVPLDKDGNVRNGNIIDLGSRNSVLFADRRVVATIGLNNMVVVDTP
                     DATLVCPKERTQDVKAVVNLLKRQGAPEHLEHRTVHRPWGSYTVMEEGKGYKVKRIEV
                     TPGKRLSLQLHHQRSEHWVVIAGTARVTRGEEVYDLQAGMSTGIAREVPHRLENPGRI
                     PLEIIEIQNGPYLGEDDIVRLQDDFGRLRPIG"
     gene            13466..>14653
                     /locus_tag="PKM72_07505"
     CDS             13466..>14653
                     /locus_tag="PKM72_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNP60668.1"
                     /translation="MGIFREYDIRGIVECDLGPDVVERIGRAYATLARARGVRNITVG
                     RDGRLTSPTLRNHLVAGLTSSGVNVLDLGLCATPLLYFSLFSCDVDGGIMITGSHNAA
                     EYNGFKMCIGKEALYGDDIQELQKIFESGEFASGSGTVLTQAIIPEYLKFLQDHFSLL
                     RGQGLRVVIDCGNGAASLVAKEALEQLGCDVTGLYCDLDGHFPNHHPDPTVLDNLKDL
                     IEKVREIGADVGIGYDGDADRIGVIDEQGQIMWGDRLLLLFARDVLDANPGCSIISEV
                     KASQGLYDDIHKQGGRGIMWKTGHSIIKAKMKEEKALLAGEMSGHLFFADRYYGYDDA
                     IYASCRLIEILVKRKKPLSSLLADIPQTVVTPEIRVDCPDDQKFSLVEIVKNRLKHAA
                     STQN"
BASE COUNT         3553 a         3136 c         3970 g         3994 t
ORIGIN      
        1 agcgagatct tcctcatcca aggtatcggc caatccgtta ttttgtaata agctgacatc
       61 ccaaaataat atcccggcag tggccggttt cccctcgatg gtggcgacca cgttaatatt
      121 atcgccatgc tcggttggag tgctcacaaa cgtcggctca gcattctgtt gcttcgcttg
      181 ccggataagc tccatgaata tttttaatct ttgtaggctg gaagggtctg tccccataaa
      241 tgtctttccc tctcatgaga agtttgaact ggttcacctg caattgggag tcctgcgagg
      301 taatcgtcga aggtatagag cctgtgggcc aatattcgga tttatgcggt tccttgatgg
      361 cctgaggtgg aaggatcctg ctggtctcgt ttccgggctc gagaggcgat gttttccatg
      421 agccccgtga gtttttgata ttggggatgg tcgggagaga gcggtttgcc atcttcatcg
      481 cacaacaggt cttgcatccc ttcgagcatg gcggccattt ccgggaaaat ttcgtcatca
      541 gaattttttc gtcccatggt gtctttcctt tgctattgat tcgagtctga ttgataagct
      601 catgccttac attaagattt gctgtcttgt ttcaggatgc tttcgatcac cgtcttcagg
      661 ttttcggcag ttaaatttgc cacgcgaatg ttcccatcca aaagtacggt tggcgtatgt
      721 gtgaccttga tccgttctcc ccaggtccgg cctttggcta atgttttgaa aggctttcca
      781 ctggcgagtc ctgtttcaaa ttctgtggga tcgagcccga tctctttcag aaggagtgag
      841 cgcatggtct tatcgaagac ttgaatgtct ttgtcgtgaa tggcctgaaa gaggagttct
      901 ttcatttcag gacctttgcc catagtcacg gcctgattgt acatttcaaa ggccgtcggc
      961 agtttgccgg gtatgacggg aaatcccacc attctaatct ctaactggtc gccaaattct
     1021 tttttcagtt gggtcccgac aacccgttcg aacatgtggc aatgggagca gtaaaagtca
     1081 gcgaattcaa acagaatgac cttgccggct tcatgctgag aaggttcccc ctccatcagt
     1141 tcgtattcgc cctgtatcgt gctcaaggcc acatttgccc atcccatcag gctaatcagg
     1201 agtattcctc caataatgcg ggcaatttgt gtctgtcctg atttcatgtt tgatcctttc
     1261 ttgatttcga ttctatccct ggaatttttc tctcgaatcg ccggagcctg ttcgggtaac
     1321 atgctacgtt tctggaggca ctcttacttt agcccatcat atcagactga acctagggtc
     1381 attcaaggtt tctggaaaag gagctgttga aaaaagccgc cagcggcgtt ctcgccattt
     1441 ttccgtgctc acgtactgga agtacgctct gcgcgcaaaa atggctgcgg ccttcccttc
     1501 ggcatgactc agtgcaggac tggacgaacg ttttttttac cgccccgagg cctctgatag
     1561 gcaacgtgcc tgcgggtgaa tatgcccttg aaaattagta ttattcaaca ttcctggaaa
     1621 atgaaatcgc gtcggggagg tccgggatgg tgaggactgg tcggcataag ggatttctga
     1681 ggattattca gagagggtct agttggtatc gacattgttc aattgaggag ggcattgacg
     1741 tgaatgtggt gaatatccaa gactacagcc agttttcgaa tgaaaagatg aagaagcaga
     1801 atgtgtttca atcaccaaga tttttttgtg atgtgtatgg ttttgagcca gggcaggaac
     1861 aaaaaggcca tgtgcatgcc gaccaggata aagtctattt ggttttggaa gggcaaggca
     1921 cgtttcgggt cgggaatgaa agccggatac tgggagaagg gcaggggacg atggctccgg
     1981 ccggggaaga gcatggcgtg ttgaaccata cgactacccg gctccgggtg ctggtcttta
     2041 tggctcccaa tccagggtaa ggcctgtaac gggttgactc attttggtgg tgtgaagaaa
     2101 ggaaaaggtg ccggggttgc gtggatggtc atagtcgact cctcatgcag aaatgtaatt
     2161 gtgagatctc atgaacgaag aacggtagca gattcctata tcttagttat ttcaagtctg
     2221 tgtgttgatc gatgcccagg gtgcgctgta ggctatgaat gtgtcaatgt tctaagaata
     2281 tccatcgcat gttcaattga tttgggggga gaggattatg acttggagtg atgccacaac
     2341 ccaggcgacc ttcatcatgt gtgctgtttt cttgatggga acgatgatgg tggggtgttc
     2401 ttccgcaccc gaacctcaac cggacccgtc caaaaaagac attcaacagg attcagatcg
     2461 gttctttaaa aagatgagcc aggaagaaaa ccaacaaact ccatcacgtt aagagagaat
     2521 ttccggaagg ctcggttcgt tttttatcgt gcccatctgt tgctctgctg ctcatgcaaa
     2581 agaagcagat attctactca aagcgtcaag ttgtgatgaa ggagtgagga ggatggctat
     2641 tcagttgagt tggtttcccg tatcagtttg gtttttgggg gtatttgatc aaggatagcc
     2701 gatcggacct ggaataaacc tggcaagctt gctcctttgg cgaagaccag tccccgttcc
     2761 ctctttccca aaattaattg tccgatttcc ttcccattgc ggttcattcc ggaaatggta
     2821 gcgttgggtg aggccatccc attgtctttt gaggcgggag aggaatcggg gtggaatatt
     2881 tccgctggag tatccacgat ccgactgaca aaaagtttta ccacttcctg atttaactct
     2941 gctgaaggat tgtcctctaa gatccactca tcgtgttgat tgatgaggac ataatgatct
     3001 tgtggggtgt ggacggtcag catagcaatg tcggcgactt ccatgccaaa gagacgtttg
     3061 tcttgaaaat gaaacatgcc ttgagtgatg ggtttcagta tccccgtggg aatttcatag
     3121 agtgggccgg aggcggacgt aatggcatag gccttttccg gatcagcaaa ttgatagagt
     3181 tccagatggt gcgtgcgtgc gccttctacg acttggatgg tggcttcaat gcgtgccggt
     3241 tgctccagaa tccgtttctt ctcttcttcg gtatcaatga accctgtggc ggccaaacct
     3301 cccaaatcca tgaggagggt gcccacggcc gttttatctg caggtccttt cacaggactt
     3361 tgaaacatcc aattcggcgt caagctatgg gctcctgcca tccgtgtcat gaccatggtg
     3421 gatgatccga gttgaatgtg tagctccagg actcgatccc gctcgaaaaa taataggtcc
     3481 ttgagccgaa agtttgtgag agacttttga gcaaaggtca tcacatccaa agtggtgagc
     3541 acgacctgat tgtcaggttt cgtttggacg tacaaggacg gagcgaaggg gccggcatcc
     3601 cctagagcta tttcctgcgt ctcagtcgga gtggtcaggg tgagggtgag ataagggggc
     3661 tctaatccgt atgctgacaa attgcttgga ccatcctcaa tataccgttt gatttttcct
     3721 atcgtgaggg ctcgtaggat cctgcgaatt tcacgcgcat ccgcagggga tcgcataggt
     3781 tcggtcatca tccaccgcca ttggtcatca cgttcgaggc ggatggtgtc cgcaggagtg
     3841 gcccaggtga tctgcgtgat agcccggtca tcaaagggca ggatctgtcg ttcctgggtt
     3901 tcctgttttt ccagttggcg ggattgaggg atgtcaatga ggaggatata gcctcccaag
     3961 ccaagaacga ccagagccag gatgagcgtc actcgaacgg gatttatcat gttacagact
     4021 tcgccgcctt ttccaaacgg ataaaccgat aaataaggta aggagtggaa ggaacaggac
     4081 ctgaaaaaac acgagcgcct gttcctgcga gggattcggt atgaatggat gaaaggccgg
     4141 atcttttggt gtcaaagaaa ctaaggcgtc ctcttgggca agccatgcga tggcctttag
     4201 gaaaaagtcc gtgtttcccg gataggtcag atacccattg gttgcaaacg ccgcattgcc
     4261 cacgatgaga acggcagatt gagctgttgg ggactggtcg accggtttac ggattaagag
     4321 gcccgcgacg gtaaaggggc ctttggggtc ctgccctgcg gtgaattcag gttgaggact
     4381 gttcatgtcc gtttcttccc agctttccgg agacgtttgt gctaaggcga catactccca
     4441 ggtctttccc tgtgccgtgt cgaaggtcac tgagcgggac acaggaaata ggattggagt
     4501 ggtgaagtct tcggtgatgt catgtgtggt aaaggtgcgt acgagtaacg cggtgggact
     4561 gcctcgtccc aagcggtcct gttcatcagc caggatgccg gctcccaatt gaatgcccca
     4621 aggtgtgatc aggggatcta acgaagcgtc actggctgga tcaaccatca ccagtacccg
     4681 tcctcctttt tccagaaatt gacgtatccg gtgttggtcc tcggcggata cggcttctat
     4741 ggaggaggcc acaatgagca ctgctgcttc gtgagaagcc gtgtcttccc aatccactgt
     4801 cccgacctca tagccttgct tggtcagggc gtcgccggca cgagataatc ctgacgattc
     4861 ggtatcggtt agactctttt cgccatggcc cgtgacaaag gcaatgcgct ttttcttgtc
     4921 ctgtgtgacg cggagcagcg cattggtcat gtcggcttca gaagaccgtt ggagatagac
     4981 cttttgggga ccactttcca cgacgacggt atcgattcga gtcacttgat actctttggc
     5041 tttgtctggt tggcgttcag gatcgatgaa agtcagggtg atgagaggac tctccttcgc
     5101 ataggtcgtc aagagatcct ggtacccccc ataacccggg ctcccttcat gggtgaagac
     5161 agtcattttt acttctcttg gcagagtccg aaggacttga taggtctgtc ggcttagggt
     5221 gaaattttga gtctctgaga aatcccattc cggggcatgc ttggctgcga gaaaattgag
     5281 taaggcaacg acgacgcctg caagcagaac cgaaaacagg ctgtgtgctc cgagtcgggt
     5341 ggaccgagtt cgagctaacg cagtaaggct gcgaaaatga aaggcaaaat agcccaggag
     5401 gcagagtgtc cccaggccga gcaggattga ggaaaggctt cctccagcag gatggaataa
     5461 ggggagacca agtcctataa gggctaatat ggttccggct attccgagaa tggaggccac
     5521 acgggttact tccatcggta ggcgtcgacg gcttggtgag cggcaaacca cataaaggct
     5581 atgccggaga cgtaatagag aatatctttg atattgaaga gaccacgaat caatcgttca
     5641 taatgttcgc taaacgagag ataggacaga atgtgcccga tggtggtgtc tccaacaatg
     5701 cctcctaacg ccccgagcat ccagagacct aagatgcagc caaagcttaa aaaagccgca
     5761 ataatttgat tctcggtcag agcggaggca aacagaccaa tggaaatgag aagggctccc
     5821 tggaggaaca gggcgacata cccaagataa atcggtcgcc aatcaaaggt tgcataggcg
     5881 gaaagtgtta ttgggatgag gcttgttagt gataacaagc cgctatagac gattatcacg
     5941 ctcatgaact ttccggaaac aatttcattc acaccaatgg gagacgtcag aaggagctcg
     6001 aaggttcgga gtttgcgttc ttcggcgaac agacgcatgg tgagtaatgg cagaataatc
     6061 atgaggatga ggttgatact tcgaaagagt ggcctgaaca acatatcatt gagattgagt
     6121 tgtgcgtagg tgttttgaat ttgcatcagg cggatggcct ggttactcgc attggctgct
     6181 gcggaatagg ccaacaggcc cacgatggct aaaaatatag cggcaatgac atagaccact
     6241 ggagacacaa atgcacttcg taattctttg gcaacaatcg tgtgaacggg ggtcataatt
     6301 aaagtaatcc ggctatctgc ggttagtgtg gaccaccagg cgagaggttg ggagcgctgg
     6361 gagtgttggt ttgtgtgagc accttaaacg catcctcaag ggtaaggggt tgggttttta
     6421 attccaggag gcccacattg cgtgagacga taaattgggt caattcctct cggatatcac
     6481 ggcccatctg cgtgtccaga agataggtgt gagctgtcgc cccttgggag actcggatga
     6541 ctccgggaat atggtgaagc gcctccaaca ggtccgggtc agattgcttt acggtcaggc
     6601 tgagcgtttc cgattcacgg agtttggccc ctagctgttc cggcgtgtcc acggcgacga
     6661 ttcgcccatg gtggatgata ataactcgtt ggcaaatagc agttgcctca gccagcagat
     6721 gtgtacttaa aataatagta tgggatcccg ccaggctttt aatgagttcc ctaatctcaa
     6781 tgatttgatt cgggtctaac cctgtcgttg gttcgtccaa aatcaacacg ggaggattgt
     6841 gcaaaagggc ttgtgccaag ccgacgcgtt gtcgatatcc acgggataga tgtccgatga
     6901 ctcgtccccg aacatcggct aggcccgttt gggtgatgat ggtatccatc cgttctgtca
     6961 attggtcatc aggaagtcgt ttaattcttc ccacaaaggt taaatattca ctaacggtca
     7021 gttccagata gagcggaggg gtttcaggga ggtatccgat atgttttttg acttcccatg
     7081 gtgtttccag gcaatcaaac cctgcaatgc gaacggtccc tagggttggg ggcataaacc
     7141 ctgtcagaat acgcatggtc gtggtttttc ctgcgccatt aggcccgaga aatgccacga
     7201 tttctccctt gttaatggag aaagagatgt catccaacgc ggttgcgttc ccgtagcgtt
     7261 tggtcacatg ttgaacgtca atcatacgga tgcccagcta gttgtgtcgt gagatggtga
     7321 gaaaagtgac tttaaacagc aatgcctcga caggagtccg atcataccta tgtgaaaaaa
     7381 aacccgtcaa gtttaaacat gaggacagtg ccgaccgaga ttgactcttt tgttgaaagt
     7441 tgtatgatag atttactttt ggagatgatt gggccgtagg agggataaaa cttcgagctt
     7501 gcctttggat attggagtaa cggccaccat tgaggaagtt gtttcaggga aattatgcag
     7561 tccttgggtt tttagttgcc cttttaagtg ggtgtagttc ctggcatcat gtggcaatcg
     7621 tcagtcatgg tgctgacttg aggagtgaat cttcctcttc cgattcttct cctccttcgt
     7681 tgggggctgc cccacccgct tctgtgccaa agccggaacc gtttcttccc ctagtttcgc
     7741 aacctacaaa atcagagaat cttccacccg aaataaactc gggtgttact gcccactctg
     7801 cggatgactc ccttctcccc ttgaagttgg aagatgtgtt ttttgactat gaccaatatt
     7861 ccatagaaaa ggaggctgtt cctaccctga agcagaacgc tcaggtactc ctgaaacgat
     7921 atccttctcg tgaagtattg attgaagggc attgtgatga acggggaacc gaggaataca
     7981 accttgttct gggtgagcga cgcgcgatgg cggtgaagaa ttaccttgtg gatttaggca
     8041 taaccgcttc ctctattcga gtccttagtt taggaaaaca tgaacccttt tgtctgcagc
     8101 ccacacttcg gtgttttcgg cagaatcggc gagcccactt tgtgttgaag caaccatgag
     8161 atcttgaact caatgcgttt cctcttcagt tggagtactg ttcagaactc atggggatcg
     8221 agtccatttt ggctgtccct ccttttaggc tattgaaggc gtccaaaccg cgggatatct
     8281 tgggagaaaa attgcaagtt tctcaaggaa attctagcac ttttcctcaa aatgaggagg
     8341 acgtctatgc ggttttggtc tctaataatc tggttcctcc tattgtttcc ctcgtcggcc
     8401 ctcccggagg tatgggattt ttcctgtacc gaggctgtct ctcttttgag ggccgctcag
     8461 gaccgggtcg tccggcagca tgaccaattg caggaagcta aatttagtct ccggcatgcc
     8521 ccaaaagaat ttgatgggtg caggcggagt cgtcgtggat ttcaaggggg aaagattcat
     8581 tgtgtgactc atcaatcccc ccaagggaat cttttgagag atatccttgt tgctcaacga
     8641 agccttgatg cctccatcca ggattttaaa aaataccaga aagatctggg tttatcctgc
     8701 tctatgcctt ctccctagcc aggtattctg ttctctccac tcttattcca ctccacccac
     8761 aaaatactgt ctcggagggt ggttctcctt cgatcctgct ttcagtgttt ggtctgagtt
     8821 aaaaccgtca acatgccatg gctgtttttt atctcttccc gaggagggcg actttaatca
     8881 ttggttcaat ctcctcccat ctcgggcaga gatcatagct gtttttgctt tcatgcttga
     8941 tgagaatgat gctgctctcg ggccaaccgt tgttttgcca aaaatgaagt attgtttacg
     9001 taaatttatg aaataatcga ccggtgccac tgaataacca ggcaaagctt ttctctcatc
     9061 gataagagaa agagattttt gatgctcagt cttaaaaaaa ttttagcctc atttttttct
     9121 cctctctctc tttgcatcga gatgattgct tgtgggcttc tccttttgtg tttttcccgg
     9181 aagcaaaatg ctgggaaaat tttcattacc ctggggttta ttctacttgt tgtcagcggt
     9241 tacgaaggag tgtcggggag aatcatacaa acgctggaat cacaatatcc tccaattaat
     9301 ctttctcagg ttatgcctcc tggaggtgtc gcaaatgccc aaggttccgt gaaatggatt
     9361 gttgtcctgg ccagcggcat tgccggagat gcgacgttac cttatcaact tcaagtgtcc
     9421 tatcattccc gtgttcggct gatggagggg attcggcttc atcgcctgct acccggcagc
     9481 aaaatcatcc tgacaggggg gactggattt gatgggtccc ctgaagcgac tgcaatgagt
     9541 agggtggccg aggaattggg cgtcagtcgg gcagatatgg ttctggaggt tgaatcccgt
     9601 gataccaagg atcatcctca ctatgttcgg gaaatcgtcc atgatgagcc ttttatcctg
     9661 gtgacaagtg ccttccatat gccacgcgcg gtcaggttat ttgagaaaca gggtctaatt
     9721 ccaattccgg catcgacagg acattgggtt cctcccatcc agttttggtc tttggtgaac
     9781 ttttttccca gttccaccgg tgtgcgcctt gccgaattgg cgtatcatga atatctggga
     9841 ttattgtggg ctcggtttca aggccagatt gaatgagtcg tgatgagacg cgctgaatct
     9901 taatctttta atgcggatgg aaaattagtt gagaaggttg atgcccctac cctgagcggg
     9961 gggagtttac agcaggcctt atttctcagc ttgggctgtc cgatagagat ggaaaagaat
    10021 gtaatcgggt aggtttcttt acttatgtta cctgagactt tttggagatc agtaaacgta
    10081 tgattgggca aaaatgtggg ggaggagccc tcattttgtg tctaagtttt ttgtctatag
    10141 gagggtgtag tatttgggaa tcgtattccc accccgacca tggtcctact cgtgctgatc
    10201 gtatttgcca tccttatgga gactgcgtgc aaggggtatg ggttgccacc gatagagcag
    10261 agacgggtac ggctgaagca catgcacact gtgttacgca ggttggcgca gagcagggga
    10321 acgattggaa gaaaaagtcg gttactcagg ggttggaaat aggtgagtgc atggaacgac
    10381 gcggattttt gctcaagcag tgatagaagg agaaagtttc attaagttat cttaagcaag
    10441 tgatctccta atactcatcc acatacctct ctcttgaccc tttcatgttg cgtgtggctg
    10501 aagaaagtaa tcatttccca tctgctaggt tttatttctc ttggccattc gaatcggtag
    10561 cggttacaca aattgtggca tgaagggttt cctctggttt aaggttgaaa agaacttttt
    10621 cttgaatggt ccccccccct cctggattgg tcgcactcat ctctttatat ttgattaatc
    10681 cttttcctat atcttggtaa atggtagtct tggcaagatt tgtgagcgtc tcgccgttgg
    10741 cttttgttgt gggttcgatg tatgaaatgt tgacaacagg gttggatccc gtcaccgtga
    10801 tattggcaga ggtgagacag gtcaaggcag cgttaatttc agaagtagag atcggcggag
    10861 atatgggttg aggctcttct gaggaaacga atgaatcggt tgggtttgaa gggatatcgg
    10921 cctgaggcgt acttgcgttg gtggatggga gttcagaggg aaggcaagag gggcgtagcc
    10981 aacataatac ggtgtccagt ctttcctgag attgtggaga tgagcaggca agaagactgg
    11041 aacaaagagc caggatcata agaaatcgga ttttaatatt cacaggtaat ggtactcttt
    11101 aaccggtttt gcaaagctgg gttgcgacaa taataaatcg gacgattgat ttttgagttt
    11161 ggctgccttt gtattctatc cctcctctgg tcttaacgga aggcttgcct taagcttagg
    11221 agtatcatca ggctaaacgg atattaaatg ggaaatagaa aggtggacat gaacaggtga
    11281 gaaaaaacca atcaattgcc cctttgtcca aaggggtaaa aacagtaaaa tctctcaaaa
    11341 aaaaagattc tccccgatcg ttaagtaagc cgaaaaaaat tttaaaagcg ctgagactgt
    11401 ttgcaacaga tgtggatggg gttctgaccg atgctggcat gtattatggg gaatcagggg
    11461 aagaattgaa aaagtttcac acccgtgatg gcatggggat taaacttcta caggcccaag
    11521 ggttgatcac cgctattatt acaatggaaa acaccaaaat cgtcgctcgg cgaggaaaaa
    11581 agcttggcat tcccgaagtt tttcagggag cgaagaacaa ggtggcggtc cttggtcatt
    11641 tgtctgaaaa atacaaaatt ccttttgcgc aaatggctta tattggagat gatgtgaatg
    11701 acgtggaggc tttgaaaact gtgggctatg ccgcagcacc cgcagattgt gtggagcagg
    11761 tccgtcaagt tgtgcattat gtttgcaaaa aaaatggagg tgagggagct gttcgggaat
    11821 ttatcgatac aattttggct gtaaaaaccc ctttgtagtt ttacaagttt ttattgtctt
    11881 tttgaacgcc ataattcgag aatttgaggt gtgggtttat aacccatacc aaaatactta
    11941 tggttttcta tttggtccat tggaggagtt gaaacgcttt ttaggaaaaa actatgaatg
    12001 ctcatgtcta tggagtgatt ttagccggag gaagtggtac ccggttttgg ccattgagcc
    12061 gggaacgatt tcccaagcaa tttttgagaa tattgggaga aggaaccctt ctacaacaaa
    12121 cctttgagcg tctattacaa aaaattcctg cgaatcggat ggcaattgtc accaacgcgg
    12181 ttcaagcgga atccatcaag ctccaactca atcaatggaa agatgacatc gctggaaatg
    12241 taattctcga gcctgaagga agaaatacgg cgccggctat cgctttagcc gccttacaat
    12301 tgatacatca agatcctgaa gcggtgatgg tggtggttcc agctgatcat gttgtgaagg
    12361 cctctaagaa attcatgcaa gccgtacaat ttgcgagtga attggcggtg gaggggcatc
    12421 ttgtgacgtt tgggattcag ccgactcgtc ccgagacagg ttatggctat attcagcctt
    12481 tgaagcggcg gaaggtaggg accaaaggcg attttatcgg ctattcggtt gggcgctttg
    12541 ttgaaaagcc gaacctcaca actgccaggc gctatttgca atctggaaac tatttttgga
    12601 atagcggaat ttttgtctgg aaagcttcac agattttatc agaaatagcc attcatcaac
    12661 cggcgttatc caagttgtta agtggtatta agcgtcgaat gggaagtgag gagttcccct
    12721 cctaccttcg gaaggtatat gccaaagtcg aatcgctttc catcgacaat gcggtgatgg
    12781 agcattcttc tcgaagcgtg gtcgtaccaa tagactttgg ttggtcggat gtggggagtt
    12841 ggagtagtct ggaggaagtg gtccccctgg ataaagatgg aaatgtccgg aatgggaaca
    12901 ttatcgatct gggaagccgc aactccgtgc tatttgcgga tcgaagggtc gttgcgacga
    12961 ttggcctcaa taatatggtg gtggtggaca cgcctgatgc cactctggtc tgtcctaaag
    13021 agcgtacgca agatgtcaaa gccgtcgtca atttgttgaa acgccaggga gcgcctgaac
    13081 atttagaaca ccgaactgtg catcggcctt ggggttccta taccgtcatg gaggagggca
    13141 aaggatacaa ggtcaagcgg attgaagtta cgcctggaaa acggctttcc cttcaactcc
    13201 atcatcaacg tagtgaacat tgggtggtca ttgccgggac cgcccgagtg acccgtggcg
    13261 aagaagtcta cgatttgcag gctggcatga gtacagggat agccagggaa gttccgcatc
    13321 gcttggaaaa tcctgggcgg atccctttag agattattga aattcaaaat gggccctacc
    13381 tgggggaaga tgatatcgtt cgacttcagg acgattttgg tagattgaga ccgattgggt
    13441 aggactcgga gggaaagaaa gccggatggg aatatttcga gagtatgata ttcgcgggat
    13501 tgttgaatgc gatttaggtc cagatgtggt tgagcgaatc ggccgtgcgt atgcgacctt
    13561 ggctagagca cgcggtgttc gcaatattac ggtgggccgg gatggtcgct taacatcacc
    13621 aacccttcga aatcatctag ttgccggcct gaccagttct ggggtgaatg tattggatct
    13681 tggcttatgt gcgaccccct tactgtattt ttccctgttt tcctgtgatg ttgacggggg
    13741 catcatgatt accggcagtc acaatgctgc tgagtataac gggttcaaga tgtgtatagg
    13801 caaggaagcc ttgtacggag atgatattca agaattacag aaaatttttg aaagtggcga
    13861 gttcgcatct gggtcaggaa cggttttaac gcaagccatc attcccgaat atcttaaatt
    13921 tttgcaagac catttttctt tgttgagggg gcaggggctt cgggtcgtga ttgattgtgg
    13981 gaatggggcg gcttctctag tggccaagga agctttagag caattgggat gcgatgtgac
    14041 cggattgtat tgcgatcttg atggtcattt tcccaaccat catccagacc ccactgtgct
    14101 ggataatctt aaggacctga ttgagaaagt cagagaaatc ggagcagatg taggaatcgg
    14161 gtacgatggt gatgctgatc gaattggcgt cattgatgaa cagggccaaa ttatgtgggg
    14221 tgatcggttg ttgcttcttt ttgcccggga tgtgctggat gccaatccag ggtgttccat
    14281 tatttcagag gtgaaagcct cacaggggtt gtatgacgat attcacaaac aaggtggacg
    14341 gggtattatg tggaagaccg gacattccat cattaaggcc aaaatgaaag aggaaaaggc
    14401 gttgctcgcc ggagagatgt cgggtcacct gttctttgcc gatcggtact atgggtatga
    14461 cgatgctatt tatgcttctt gtcggctaat cgaaattctt gtgaagcgga aaaagccatt
    14521 gtcttccctg ttggcggata ttccccaaac ggtggtgacg ccagaaattc gggtagattg
    14581 tccggatgat cagaaatttt cattggtaga aatcgtcaaa aatcggttga agcacgcggc
    14641 cagcacacag aat
//
LOCUS       DAORAQ010000062        14610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_698_length_14610_cov_64.879423, whole genome shotgun sequence.
ACCESSION   DAORAQ010000062 DAORAQ010000000
VERSION     DAORAQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_14610_cov_64.879423"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1114
                     /locus_tag="PKM72_07510"
     CDS             131..1114
                     /locus_tag="PKM72_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNP60669.1"
                     /translation="MEPTSKIYIAGHMGMMGSAFLRQLQARGQTHILTRTRRELDLTN
                     QAAVQGFFENEKPDYVFVAAAKVGGIMANATYPADFIYQNLAIQTNILHAAYLTGVKR
                     VLFLGSVCIYPKHCPQPIKEDYLLTGALEPTNEPYAIAKIAGLKMCEAYNRQYGTQYL
                     CAMPTNLYGSGDNFDLETSHVLPALIRKFHELKVSGTPVTLWGTGQASREFLHVDDCA
                     NACLFLMDLDPERFQTLLEYHAGPLVNVGSGEDISIRGLAEMIKELIGASGQINWDST
                     KPDGTPKRKLDITRLHALGWQPNISLSNGIKRTYDWYLRHDTPGQFMEAHS"
     gene            1111..1947
                     /locus_tag="PKM72_07515"
     CDS             1111..1947
                     /locus_tag="PKM72_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNP60670.1"
                     /translation="MNTIGMLPSTSPSELSDSTKGMARQHSPCPPKFNLFGIGVSRTD
                     YQEATILLIQAAQERRSLIVDHMPVHGLIEACSDSSLKNKINQFDIVAPDGQPVRWAL
                     NRFHQANLSDRVYGPELMLRLCHQAEQKGVGVYLYGSQPEVLGGLKRNLLHRFPSLRI
                     VGAEAPPFRPLTQDEDQAAVARINASGAGLIFLGLGCPKQEHFAFDHREKIHGVQLCV
                     GAAFDFHAGTKEIAPPWMQRNSLEWVFRLWCEPKRLWKRYFVTNSIFLWKILRHEFGI
                     LK"
     gene            2134..>2826
                     /locus_tag="PKM72_07520"
     CDS             2134..>2826
                     /locus_tag="PKM72_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNP60671.1"
                     /translation="MKILCLGGAGFIGSHLTARLLKERHTVTVVDQAHDKIQEWVGFD
                     NLRIVKEDIRRPDWGLRELVQDADVVIDLIAYANPGLYVKMPLEVFRLNFTENLKIAE
                     TCVALDKRLIQFSTCEVYGKTPASFLNGHLLNSDDPRHATFSEDSTNLIMGPVNKHRW
                     IYASAKQLLERVLHAYGLELGFNYTIIRPFNFIGPKIDFLLSEGEGIPRVFSFFMDAL
                     LTGTNMKLVNGGH"
     assembly_gap    2827..2926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2927..3115
                     /locus_tag="PKM72_07525"
     CDS             <2927..3115
                     /locus_tag="PKM72_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60672.1"
                     /translation="FYGKGYEDSDRRIPDISKAEALIGWKPQWGLNELLENTMDYYVN
                     HVNTTQAKIAPALKETHI"
     gene            3256..4407
                     /locus_tag="PKM72_07530"
     CDS             3256..4407
                     /locus_tag="PKM72_07530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP60673.1"
                     /translation="MKILHIFHDLLETGGTPFQVRSLVQAQADLGNDVLAVSYKGDSE
                     RISSNVQSVQLGKGLRSVLQFRKVLQSFKPHIIHLSGIWIPVHHVWLKEVFRFGAPCI
                     ISTNGNLSPHGFGVRFGGKRTHFYHIWRKELWHKYFDAPMLRAVACVHTPSKYEAEIL
                     AELGVTKRFIAPVGINASWIKSQPCDGRKLNDPVTFLHLGRLDIFHKGLDLVCDAVEV
                     LMRSPNPKPFRVIMAGPSVNDSMEKLKKRVAGIGSEIFDFRDYVSGEEKKMLWTEADY
                     FLNVYRYAGIALAPCEALGQGIPLIASWEGNLGDWTQRMNMGITVPLSGTHLADGMAT
                     VLEYSEDVYQRLSENAMKFCLSYSWEKVAQDLVDEYKQIIDDYSCGVMP"
     gene            4463..>5292
                     /locus_tag="PKM72_07535"
     CDS             4463..>5292
                     /locus_tag="PKM72_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNP60674.1"
                     /translation="MVLNRWGKRGGISLVDQAIHGGTNFLINIILARWLSPNEYGAFA
                     VAYTIFLLIGAIHTSVMIEPMMVYGAKKYIETFRGYIGSLLLGHGVFSVLVTIILIVS
                     SLTFLAFDSKELADAFLGLAIASPFILLQWMIRRSFYVVSQSTWALAGSFMYFTLNAL
                     VIFLLFQKASLTPYWAFLSMGLIGFLVSVCQLRILKPEFKWTGPLSLYSIFHDHKEFG
                     AWNIVGTVIKWASTGILTIMVSAFLGLKAAGVLSVVRTLFRPLDLMLNAVSLVALPVL
                     S"
     assembly_gap    5293..5392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5417..6601
                     /locus_tag="PKM72_07540"
     CDS             5417..6601
                     /locus_tag="PKM72_07540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP60675.1"
                     /translation="MKILHWYPNLLRGGAVANGVLGLSHSQAKLGADVVIATASTTTE
                     PLYYSIERPKDVELFEWHPRWFINFQGRGLRILSRNTIKKLRSFEPEIVHVHGEFNID
                     NLHACRIFHCPVVLSPRGAFAPRLLKRGVGLWGGKYAYLKLAKKILYEKVVAFHGLSP
                     LEKVQIEELLPTSHVYCAAQGPNLQLEKYLIPGVREESFDAIQLICVGRLDVYTKGLD
                     YLVQAFAYAVKRMQGKRLCLRLIGPDCRGGKEHLKRLARSLGVLGQITFEGVVIGKEL
                     IQALKASDIYVQMSRYDGFGQSVCEALLMNKPAILSREIGCISYADIEDMSHIRKVHL
                     NGGEAGDAIIDFAGRLNELNSLAKFHSSQLKTIFSWESAAQAHLNAYAQFSGSRLPAY
                     SN"
     gene            6639..8000
                     /locus_tag="PKM72_07545"
     CDS             6639..8000
                     /locus_tag="PKM72_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60676.1"
                     /translation="MRQAAPIVLSDGVLASNLFRLFEKEGIGYVLVGDSREYPAKILS
                     DVDVVIEPECVSKLVPCLMKFCDLYNLKLIQILQHEQSAWFFVCSWTGKDGKHHVFYP
                     DVCTDYVRWGRRFLGARCLLSGRIRKQVEADGQQCSFFVPAPEQAFIYYLLKKIDKGF
                     LAPSHQSYLSGEWKKAQKESKKQLRKFWKEPEVEMLCDAAESNSWNEVCDALPRLRVA
                     LRENLPFSFKSFALDVQRKILRIMKPTGVHVVLLGPDGSGKSTILECIEGTLASAFRR
                     TKRYHLRPFFGSPQHAREPEPNPHGKPVRGFLPSLIKLGVWWIDYTIGYFISVFPFLV
                     RSTLVLFDRYYFDLAIDPRRYRYGGPLWLAKLCGKFIPRPDLVILLDVPAEELYSRKA
                     EVSVEESHRQRLAYREFVGTLTNGHIIDNSDRLEETIQKVEAVVLNYMAIRAISSLGH
                     VKH"
     gene            8002..9024
                     /locus_tag="PKM72_07550"
     CDS             8002..9024
                     /locus_tag="PKM72_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNP60677.1"
                     /translation="MRALSLFPPDAEIRALPTSKSPRLLLAGSTVKRRWQESAFYPAH
                     RRIAKCYRFFLRLKVATGLGLMAPVVNHHASFNELIGDIFPDVDSAALLIGTAGPNQK
                     NTIQLWSRERGVVGYIKYAETHIARSRLANEFKMLECIPKGVGPEVLKYTSFNDGNAL
                     VLSPIVGRAMPVSMHPPSWIHEFLNGLQISPCEGIEEHPGVKIFQAKYGSAVLPWLES
                     LSHRKWPVVFYHGDFAPWNLLHWSGRNAAAIDWEYGLSESIPHLDLIHYLLQVGRLIR
                     FWTPRQTRVFVINYMLRHFSLDLEEIEGLVRLLAYHVYREAIADGHLENTNRVLWWRS
                     VWECEK"
     gene            9006..10259
                     /locus_tag="PKM72_07555"
     CDS             9006..10259
                     /locus_tag="PKM72_07555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP60678.1"
                     /translation="MGMRKVKRLKVLISAYACEPGKGSESGVGWNVALQMAKYHDVWV
                     ITRINNRSSIEAALEQYDQPKPHFLYYDLPRWMRFWKKGGRGVRAYYYMWQMGILVLI
                     RRLHRTMTFELIHHVTFVRFWTPSFLSFLPIPFLWGPIGGGDTTPKVLRNQLDFRSKL
                     YETGRHLALWIGEHDPFVWRTAARSRMGIATTKGTANRLKRLGVSNVKVYGEAGLEKS
                     AIAQLSQSSQRSDSSFRFVTIGRLLGFKGVFFALRAFAHANIPHCEYWIIGDGPDKRR
                     LERLVVSLGIAERVKFFGEIPRSEALRKLAECNVLVHPSFHESGGWVCLEAMAAARPV
                     ICLDHSGPATQVTDETGILIAVNSAEQVLRDMGESMKRLAGAPSLCEQMGRAGRERVL
                     QDYSWDSKVDVLNEFYQEVAFQGTS"
     gene            10405..11604
                     /locus_tag="PKM72_07560"
     CDS             10405..11604
                     /locus_tag="PKM72_07560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP60679.1"
                     /translation="MENKLRVLLAHNFYQEPGGEDEVFAAEGRLLEANGHKVIRYTLH
                     NDSIQNCSKFDLAKTLIWNTKAYHDLRKIIQRERPHIAHFHNVFPLISPSAFYASRAE
                     RVPVILTLHNYRLLCSNSLFYRDEAVCEDCLKSTFPVSGVIHGCYRESRVESAGVAAM
                     LSIHRILGSWKKKVSIFIALTDFAKQKFIEGGLPEGKVVVKPNFVEPDPGIGREKVQD
                     YALFVGRLSSEKGLSTLLGAWSELHDRIPLKIVGDGPLASQVRSASSALRGVEWLGRL
                     QKEEVLLAMKQAMFLVFPSVWYEGLPMTIIEAFSVGCPVVGSAIGGVKDLIASGKTGF
                     HFRAGDVEDLVKQVTWVLAHPTQVLEMRREARQEYETHYSSAGNYRNLINIYAQARSG
                     EEGQVFS"
     gene            11646..>12871
                     /locus_tag="PKM72_07565"
     CDS             11646..>12871
                     /locus_tag="PKM72_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60680.1"
                     /translation="MNVIVELLGMRSWLFGLLALSTVLYGRILTELGGPAVVKFFHFA
                     LVAIFAILLLPFLSNSISWSLIYGLLLLSWVMVLSAVINGAGLLNILLDFLILSEPFL
                     LLVLMINRPMSKSQIQTFRWLLVVFICIHVGLSFAQALVGGGTDDVKGVFLEQGAGHH
                     VAGAVALTAAVYFFTALENFSLPVRAIISLIIASVVILSDSKQVIAVFLLGLGCLLCT
                     KLGDIRKFFIYVLLFMTSGAVLLFSAFTIFPALTTWADLNLILKGLTVKFGILPLLFS
                     HYDSVLNWIFGLGPGHTVGKLAFMLPQYEEQLRSLSVSFSSVTQHAWNYQQGNYLSHS
                     VTGSSMWSPFFFWAGLWGDLGILGVVSYGLLWGIVILRICTNDLARFLAFTVLIFGIV
                     FSWLEEPGYMLFVVTI"
     assembly_gap    12872..12971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12972..14550)
                     /locus_tag="PKM72_07570"
     CDS             complement(<12972..14550)
                     /locus_tag="PKM72_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNP60681.1"
                     /translation="MQASSYGAPLPLGLIEVDRTIYVNNKHLVASDSNPGTADLPLKT
                     LGQATTRAIENRELGLGTKILVAPGVYREKFQLIVNPQDSDPPIVFEATEIGKAIISG
                     ADVFRNWKPTKIPNIFVHAWPYKWGVKPLPDSWKGRVNVSHIVRRKEMVFVNGIPLKQ
                     VLSAIELVAGTFFATESTSQLFIALPEGLAPSESLIEVAIRSRPVLIKGKTNLVLKGL
                     TFQHAASDYLGSALVITDGNNVTLEDLLFSWNNGMGYFLHNIQNLTSKRNETSNNGAS
                     GYQISTIKNLLSEDDKALRNNWRGVKGEFVNWGVAGFKGLKIHDAIFRRHMAQGNHTT
                     GFWLDYDNRNIRMEDGRWCGNRTYGLFVEASERITITNAKIYQNNQWGFRTVQTPDLT
                     LTGSRIYANRGYQLIVQAPFTRMVNNWETGERIEHKTERWQLTNNIISGDHALLITDP
                     HFIFLNGLFSDGNIWMKPSHPGAFRLGEGGKSIRFPEWQSTAKQDQNSFFIHPDSLAT
                     NLAMDCIYPPAPPPTLTK"
BASE COUNT         3612 a         2930 c         3525 g         4243 t
ORIGIN      
        1 aagtggtgga agggtgatta gtcatttcag gatactgaga atgcagcact catgtaccca
       61 ttttatccaa ttcacctgcg gcaggaacac ctcagttgtg ccatgctaat tctagccgta
      121 atgctcaaca atggaaccaa ccagtaaaat atacatagcc ggtcatatgg gaatgatggg
      181 ttcggccttt ctgcgtcaac ttcaggccag agggcagacg catatcctta cacgaactcg
      241 tcgtgaactg gacttgacga atcaagctgc agtccaaggc ttttttgaaa atgaaaaacc
      301 ggattatgta tttgtggcgg cagccaaggt cggaggtatc atggccaatg ccacttatcc
      361 cgccgatttc atttatcaga acctggcgat tcaaaccaat attttacatg cggcctatct
      421 caccggggtg aagcgagttt tgtttttggg aagcgtgtgt atttatccca agcattgtcc
      481 ccaaccgatt aaagaagact atctgttgac cggcgcgctt gagccgacta atgaacccta
      541 tgccattgcc aaaattgcag gattaaaaat gtgcgaggcg tataatcgac aatacgggac
      601 gcaatatttg tgtgcgatgc caacgaacct ctatggaagc ggggataatt ttgatctgga
      661 aaccagccat gtgttacctg cactcatcag gaaatttcat gaattaaagg tttctggcac
      721 tcctgttacg ctttggggca caggtcaagc cagccgggaa tttcttcatg ttgatgactg
      781 tgcgaacgct tgccttttcc tcatggattt ggatcctgag cgatttcaga ccttgctcga
      841 atatcatgcc ggtcccttgg tgaatgtcgg gagtggagag gacattagta tccggggtct
      901 ggctgaaatg attaaagagc ttattggagc atccgggcag atcaattggg attctactaa
      961 accagacgga acccccaagc ggaaactgga tataacccgt ctccatgcgc tgggttggca
     1021 gcccaatatt tctctttcca acggcataaa aaggacctat gactggtatt tgcgacacga
     1081 tacgcctggg caattcatgg aggctcattc ttgaatacta tcggaatgct cccctccacc
     1141 agtccctctg aattatctga ttccacgaaa gggatggcca ggcaacattc tccttgtccg
     1201 ccaaaattta atctattcgg tatcggagtc agtcggactg attatcagga agccaccatt
     1261 ctcctgattc aggcagctca agaacgccgc tctctgatcg tggatcatat gcctgtgcat
     1321 ggcctcatcg aagcctgttc agattcttcc ttgaagaata aaatcaacca gttcgatatt
     1381 gtcgccccgg atgggcagcc ggttcggtgg gcattgaatc gatttcatca agccaatctt
     1441 tctgaccgtg tatatggtcc cgagttaatg ctgcggttat gtcatcaagc agagcaaaaa
     1501 ggagttggtg tctatctcta tggaagccag ccggaagtct tggggggatt gaaacgaaat
     1561 cttcttcacc ggtttccttc gctacgaatc gtgggcgccg aagctcctcc ctttcgaccg
     1621 ctcacccagg atgaggatca ggcggctgtg gctcgaatca atgcgagtgg cgccgggctt
     1681 atttttttgg gtctgggatg tccgaaacag gagcattttg cttttgatca tcgtgaaaaa
     1741 atccacggtg tgcaattgtg cgtcggtgcg gcctttgatt ttcatgccgg cacgaaggaa
     1801 atagcaccgc cttggatgca acgaaacagc ttagagtggg tatttcgttt gtggtgcgag
     1861 cccaagagat tatggaagcg gtattttgtc acgaattcca tctttttatg gaaaatacta
     1921 aggcatgaat tcggtatttt gaaatgatca atctgaaaat ggtttcttcc gtattctttg
     1981 cctcaaggct gggttcttat tctaaccggt tcaatcgata tgcataaatt ttaattgcta
     2041 aaattaacac ctacatcttt atctatcata tattcctaac atacatatct atattatatt
     2101 tctaacatac acaatattat cgtgaggtag actgtgaaaa tactttgcct tgggggagcc
     2161 ggatttattg gatcccatct aacagctcga ctccttaaag aaagacatac tgtcactgtg
     2221 gtcgatcaag cccacgacaa gattcaagag tgggtcggtt ttgataatct gagaatcgtc
     2281 aaagaagata ttcgaaggcc agattggggg ttgagggaac tggttcaaga cgcggatgtg
     2341 gtcatagatt tgattgccta tgcgaatccc gggctttacg taaaaatgcc attagaggtt
     2401 tttcggttaa atttcaccga aaatttgaaa attgccgaaa cctgcgtagc attggataaa
     2461 cgattaattc agttttcgac gtgcgaggta tatggcaaaa cccctgctag cttcctgaac
     2521 ggtcatttgc tgaattctga cgatccccga catgccactt tctcggaaga tagcacaaac
     2581 ctcatcatgg ggccggtgaa taagcaccgt tggatttatg catcagctaa gcaattgttg
     2641 gagcgagtgc ttcacgccta tggtctagaa ctgggtttta actacacgat tatacgtcca
     2701 tttaatttta ttggccccaa gattgatttt ctcctaagtg aaggggaggg gataccgcga
     2761 gtcttttctt tttttatgga tgcgcttctc actggaacca atatgaagct cgtgaatggg
     2821 ggacatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttct atggtaaagg
     2941 ctatgaggat tcagataggc gaatccctga catttcaaag gcagaagcat taattgggtg
     3001 gaaacctcaa tggggattaa acgaattgtt agagaatacg atggattact acgtgaatca
     3061 cgtgaacacg acacaggcaa agatagcacc ggcactgaag gaaacacata tttagtatat
     3121 gaatgaacaa tggaatgccc ttctctcact ttctttaccc aggattaatc ccaaaattag
     3181 ttcttcctat ctgccatgac ctacgacctc agggctgatg agcggccttt tagtttgtag
     3241 gcctttcctg tcgacgtgaa aatacttcat atatttcatg atttacttga aaccggaggg
     3301 actccctttc aagtaagaag cttggtccag gctcaggctg atcttggtaa cgatgttctt
     3361 gctgtctcgt ataagggtga ctctgaaaga atttcctcaa atgttcagtc cgttcaattg
     3421 ggaaaaggtt tacgcagtgt gttgcaattt cggaaggtct tgcaatcgtt taaacctcac
     3481 atcattcatt tatccggtat ctggattccg gttcatcatg tctggttgaa agaggttttt
     3541 cgttttggtg caccctgtat tatttcaacg aacgggaatt tgagccccca tggattcggt
     3601 gtccgttttg gaggaaagcg aacgcacttt taccatattt ggagaaaaga actatggcat
     3661 aagtattttg atgccccaat gcttagagcc gtagcatgtg ttcatacccc atcaaaatac
     3721 gaagcggaaa ttctggcaga gcttggagta acaaaacggt ttatagcgcc ggtcggtatt
     3781 aatgcatcct ggataaagtc tcagccatgt gatggtagga aattgaacga tccggtgacc
     3841 tttctccatt taggtcgact ggatattttc cataaaggat tagatttagt ttgcgatgcg
     3901 gttgaggttc tgatgcgttc cccgaacccc aaaccattcc gtgtcataat ggctggtccg
     3961 tcggtaaatg attctatgga aaaacttaaa aagcgtgtag cggggatagg aagcgagatt
     4021 tttgatttta gagattatgt ctcaggggaa gagaagaaga tgctttggac cgaagcagat
     4081 tattttctta atgtgtatcg ttatgctggc attgcgcttg ctccctgtga agccttaggg
     4141 cagggcattc ctctcattgc ctcatgggaa gggaatcttg gggattggac gcagcgaatg
     4201 aacatgggaa taaccgtgcc cctgagtggt acgcatttgg ctgatgggat ggctacggtt
     4261 ttggaatatt cagaggatgt gtatcaacgg ctttccgaga atgcaatgaa attttgtttg
     4321 agctactcct gggaaaaagt agcgcaagac ttggttgatg aatataaaca aatcatcgat
     4381 gactattctt gcggggtgat gccatgagtt tgagtagagg gtcttgtgtc gtgttgttta
     4441 aatctgaatc cgcattaccg tgatggtatt gaatcgttgg gggaaaagag gcggcatttc
     4501 acttgttgac caggcgatac atggcggaac gaattttctt atcaacatta ttctcgctcg
     4561 ctggttgagc ccgaacgaat atggcgcgtt tgccgtagca tatacaattt tccttcttat
     4621 tggcgccatt cacacctcag tgatgataga accgatgatg gtatatgggg caaagaaata
     4681 catcgaaaca ttcagagggt atatcgggag tctcttgtta ggacacggag tgttctcggt
     4741 tttagtgact attattttaa ttgtttcttc cctaacattc ttggcttttg actctaagga
     4801 gctggctgat gcctttctgg gcttagctat tgcaagccct tttattcttc tacagtggat
     4861 gattcggcga agtttctatg tcgtttccca gtcgacgtgg gcccttgcag gaagttttat
     4921 gtatttcaca ttgaatgcat tggtgatatt tctccttttt cagaaggctt ccctcacccc
     4981 gtattgggca tttttgtcga tgggtcttat cggtttcctt gtgagtgttt gtcagttgcg
     5041 catacttaaa cctgagttta aatggactgg gcctttaagt ctttattcga ttttccatga
     5101 tcataaggaa ttcggagcat ggaatattgt cggaacagtt atcaaatggg cgtcgaccgg
     5161 tattttaaca attatggtat cggcctttct tggtctaaaa gcagctggag tgttgtctgt
     5221 agtgcgaacc cttttccgac cgctagatct tatgttgaat gctgtttcac tcgttgcatt
     5281 acccgtactc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgcatag
     5401 aaaagggaaa aattagttga agattttgca ttggtatccc aatcttttgc gaggtggtgc
     5461 tgttgcgaat ggcgtattgg gtttatccca tagtcaggcg aaactcgggg cggatgttgt
     5521 cattgcgact gccagcacca ccaccgaacc attgtattat tccatagagc gtcccaagga
     5581 tgtagagctc tttgagtggc acccaaggtg gtttataaat tttcaagggc gaggattgcg
     5641 tatcctatcc agaaatacga ttaagaaatt gcgatcattt gaacccgaaa ttgtgcacgt
     5701 ccatggcgaa tttaacatag ataacttgca tgcctgccga atatttcact gtccagttgt
     5761 attgagcccc cgtggagcat ttgccccacg attattaaaa agaggagttg gcctgtgggg
     5821 tgggaaatat gcctatctta aactagcaaa aaagatttta tatgaaaaag tcgttgcatt
     5881 ccacgggctt tctccattgg aaaaagtgca aattgaggaa cttcttccaa cgagtcacgt
     5941 ctattgtgcg gctcaaggac caaaccttca attagaaaaa tacctgatac ccggagtgag
     6001 agaggaaagc tttgacgcaa tacaattgat ttgtgtgggt agattggatg tctacacaaa
     6061 aggattagat tacttggtgc aggcttttgc ctatgccgtg aaacgtatgc aaggaaaacg
     6121 gctttgtcta agactcattg gcccagattg taggggcgga aaagaacacc tgaagcgact
     6181 ggcccgaagc ttaggtgtcc ttgggcaaat cacgttcgaa ggtgtggtca taggaaaaga
     6241 actcatccaa gcattgaagg catctgatat ttatgtccag atgtcgagat atgacggttt
     6301 tgggcaaagt gtctgtgaag ccctgttgat gaataagcct gctatccttt ctcgggaaat
     6361 cggttgtatc tcatatgcag atattgagga tatgtcgcat ataaggaaag ttcatctaaa
     6421 tggtggtgaa gcgggtgatg ccatcattga ctttgctgga cgccttaatg aacttaactc
     6481 tttggcaaaa tttcatagca gtcagttaaa gaccattttt tcttgggaaa gcgcagccca
     6541 ggcccacctt aatgcctatg cgcagttttc cggaagtcgc ctgccagcgt acagtaatta
     6601 atctcatgca aagggacaaa gtgttggaaa aggaccgaat gagacaggcc gcgccgatag
     6661 tcctgtctga tggggttttg gcatcaaacc tctttcgact ttttgaaaaa gagggaattg
     6721 gttatgtatt ggtgggggat tctcgggagt atccagcaaa aatattgagt gatgtggatg
     6781 tagtgattga gccggagtgc gttagtaagc ttgttccctg tctgatgaaa ttttgtgatc
     6841 tttataattt aaaacttatt cagattcttc agcatgaaca atcggcatgg ttttttgttt
     6901 gctcctggac cggtaaggat ggcaagcacc atgtttttta cccggatgtt tgcactgatt
     6961 atgttcgatg gggaaggcga ttcttagggg ctcgctgctt gttgtccggg cgtattcgaa
     7021 agcaggttga ggcagatggt cagcaatgtt ctttttttgt acccgcccct gaacaggcgt
     7081 ttatctatta cctattgaaa aaaattgata aaggtttttt ggctcccagt catcaatcct
     7141 atctgagcgg agaatggaaa aaagcgcaga aggaatcaaa gaagcaattg agaaaatttt
     7201 ggaaggagcc ggaggtagaa atgctttgtg acgcagcaga atctaattcg tggaatgagg
     7261 tgtgtgatgc attgcctagg cttcgagtcg ctcttcgcga aaatctcccc ttttcattca
     7321 aaagttttgc tttagatgtg caaagaaaaa ttctaagaat aatgaagcca acaggagttc
     7381 atgttgtcct tcttggtcca gatgggtctg ggaaaagcac cattctggaa tgtattgaag
     7441 gaacactggc atctgccttt cgtaggacca agcggtatca tctcaggcca ttttttggaa
     7501 gtcctcaaca tgctcgagaa ccagaaccga atccgcatgg taaaccggtg aggggattcc
     7561 tgccttcctt gattaagctt ggggtttggt ggattgatta taccatcggg tattttatca
     7621 gtgtgtttcc ttttttggtt cgttcgacgc ttgtattgtt tgatcggtat tatttcgatc
     7681 tagccattga tcctagacga tatcgatatg gtggtcctct ttggttagcg aagctttgtg
     7741 gaaaattcat tcctcggccg gatttagtga tattgttgga tgttccggca gaggaattgt
     7801 attcccgaaa agcagaagtt tcggtagagg aaagccatcg acaacgactt gcataccgtg
     7861 aatttgttgg caccctgaca aatggacata ttatagataa ctcggatcga ttagaggaaa
     7921 cgatccagaa ggttgaggct gtcgtactca attatatggc gatccgtgcc atctccagtc
     7981 ttggtcatgt taagcactga gatgagggct ctaagccttt ttcctccgga tgcagaaatt
     8041 agggctctcc ccacttccaa aagcccgcgg ttgcttcttg ctgggtcgac cgtaaaacgt
     8101 cgctggcagg aaagtgcttt ttatcctgct catagaagaa ttgccaagtg ttaccgtttc
     8161 ttcttgcgtt taaaagtggc aacaggattg ggtttgatgg cgcccgtggt aaatcatcat
     8221 gcaagtttca acgaattgat aggcgacatt tttcctgatg tggattccgc tgcactactc
     8281 attgggactg caggacccaa tcagaaaaat actatccaat tatggagtcg agagaggggc
     8341 gtcgttgggt acataaagta tgccgagacg cacattgctc gctccaggct agcaaacgag
     8401 ttcaaaatgt tggaatgtat tcctaaggga gttggtccag aggtattgaa gtacacatca
     8461 tttaacgacg ggaatgccct ggttctcagt cccattgttg gacgtgctat gccagtaagt
     8521 atgcatccgc catcctggat tcacgaattt ttgaatggcc ttcagatctc accctgcgaa
     8581 ggaatagaag aacaccctgg agtaaaaatt ttccaggcga aatacggcag tgctgtccta
     8641 ccttggcttg agtctctttc tcatcgaaaa tggcccgttg tgttttatca tggtgatttt
     8701 gcaccttgga acctgctgca ttggtcaggt agaaatgccg ctgcgattga ttgggagtac
     8761 ggattatcag aatccattcc gcaccttgat ttaatccatt accttttaca agtagggcga
     8821 ttgatccggt tttggactcc ccgccaaaca agggtatttg tcattaacta catgctgaga
     8881 cacttctcat tagaccttga ggagatcgaa ggattagtga ggctgttagc ctatcatgtt
     8941 tatcgtgagg caattgctga cggtcatttg gaaaatacaa atcgtgttct ttggtggagg
     9001 tccgtatggg aatgcgaaaa gtgaagcgac tgaaagtcct gatctcagcc tatgcttgtg
     9061 agccggggaa aggttcggag tctggtgtcg gctggaatgt ggctctgcaa atggcaaaat
     9121 atcatgatgt ctgggtgatt actcgcatca ataaccgatc tagcatagaa gccgcattag
     9181 agcagtatga tcaacctaag cctcatttcc tctattatga tcttccccga tggatgcgtt
     9241 tctggaagaa ggggggaaga ggcgtcagag cctattatta tatgtggcaa atgggaattt
     9301 tggtattgat ccggaggctg catcgcacaa tgaccttcga actaatacac catgtgacat
     9361 tcgtgagatt ttggaccccg agttttctct cttttctccc cattccattt ttgtggggac
     9421 ccattggtgg aggagacact acgccaaagg tgttgcgtaa tcagttggac tttaggagta
     9481 aactctacga gacaggtcga catctggctt tgtggattgg agaacatgat ccgtttgtgt
     9541 ggcgcacagc agcaagaagt cgtatgggca ttgccacgac aaaaggaacc gcaaaccgct
     9601 taaaaaggct gggggtttct aatgtaaaag tctacggtga ggcaggacta gagaagagtg
     9661 caattgccca actctcgcaa tcctcgcaac ggtcggattc ttcgtttagg tttgtgacaa
     9721 ttggaagact cttaggcttt aaaggggttt tctttgcgtt acgagcattt gctcacgcaa
     9781 atattccgca ttgtgaatat tggatcattg gtgatggtcc ggataaaaga agattggagc
     9841 gactagtcgt ctctcttggt attgcagaac gggtgaaatt cttcggcgag attcccaggt
     9901 ccgaagcgct gcgtaaactt gccgaatgca acgtccttgt ccaccccagt ttccatgagt
     9961 caggtggttg ggtgtgtttg gaggccatgg ctgctgcacg acccgtcatt tgcttagatc
    10021 atagcgggcc tgccacccaa gtaacagatg aaaccggaat cctcattgct gtaaattctg
    10081 ccgagcaggt attgcgggat atgggggagt caatgaagag gttagctggt gcgccttctc
    10141 tttgcgagca aatgggcaga gcaggcaggg aaagagtgtt gcaagattat agctgggatt
    10201 caaaggttga tgttctcaac gagttttacc aggaagtggc atttcaggga acgtcttgaa
    10261 acgattgtgt catggtctcc taagaccccg catgccacaa cgccagaaaa ccttattctt
    10321 tatcgatagg cctcagatta acccctcatt caattttctt atccatagac tactgctcta
    10381 gataagacgt gccccataac aacaatggaa aataagctaa gggttttgct tgcccacaat
    10441 ttttatcaag agccgggtgg ggaagatgaa gtttttgctg cagaaggcag gctcctcgaa
    10501 gcgaatggcc ataaggtcat tcgatatacc ttacataacg actccattca gaattgttcg
    10561 aagtttgatc tcgccaaaac gctcatttgg aataccaagg cgtatcatga tcttcgtaag
    10621 atcatccaac gggaacgacc gcacattgct catttccata atgtcttccc gttaatctct
    10681 ccttcggcat tttatgcaag ccgggcggag agagtccccg tgattttaac cttacacaat
    10741 tatcggctcc tgtgctccaa ctcacttttt tatagggacg aagccgtatg tgaggattgc
    10801 ctgaaaagta ccttcccagt ttctggagtt attcatggat gttaccgaga aagtcgcgta
    10861 gaaagtgctg gggtagcagc catgcttagt atccatcgaa ttttggggag ctggaagaag
    10921 aaggtcagta tttttatcgc actaacggat tttgcgaagc aaaagtttat tgaaggagga
    10981 ttaccagagg gaaaagttgt ggttaaacca aactttgtcg agccggatcc agggattggg
    11041 agggagaaag tacaagatta cgcgttgttc gttgggcgcc tttcctccga gaaaggtctt
    11101 agtacactgt taggtgcctg gtcagagctc catgatcgga tcccactgaa gatcgtcggc
    11161 gatggaccgc ttgcttccca ggttcgttct gcttcaagtg ctcttagggg agtcgagtgg
    11221 ttaggacggt tgcagaagga ggaggtctta ttggccatga agcaggcgat gtttttggtc
    11281 ttcccatccg tttggtacga ggggcttcct atgacaatca ttgaagcatt ttctgtcggg
    11341 tgccccgttg ttggtagtgc tattggagga gtgaaagacc tgattgcttc tggaaaaacc
    11401 ggttttcatt ttcgtgccgg ggatgtagag gacctggtga aacaagtcac ctgggttctt
    11461 gctcacccta cccaagtgtt ggagatgcgg agggaagcta gacaggagta tgaaacacat
    11521 tactcgagtg caggaaacta tagaaattta ataaatattt acgcccaggc tagaagtggg
    11581 gaagagggcc aggtcttttc ataatagtaa gcgcaagcag gccaaaaatt gagcaataga
    11641 aaacgatgaa cgtcattgta gaattactag gtatgagaag ctggctgttc gggttgcttg
    11701 cgctatccac agtactgtat ggaagaattt tgacagagtt aggcgggccg gcagttgtga
    11761 agttttttca ttttgcctta gtcgccattt tcgccattct tcttcttccg tttttgagta
    11821 attctatttc ctggtcgctt atttatgggc ttttattgct ctcgtgggtt atggttttaa
    11881 gtgcagtcat caacggtgct ggcttattaa atattttgct cgatttcctg atactttccg
    11941 agccatttct tcttttagtc ctgatgatca atcgaccgat gtccaaatca cagattcaga
    12001 cctttcgttg gctacttgtg gtttttattt gtatccatgt tggattatcg tttgctcagg
    12061 ctctcgtagg tggaggaacc gatgacgtta aaggtgtgtt tcttgaacag ggcgctgggc
    12121 accatgttgc cggggccgtt gctctgacag cagcagtata tttttttacg gcccttgaaa
    12181 atttttcttt acctgtacgg gccattatta gtctcattat agctagtgtg gtcattcttt
    12241 cggactcaaa acaagtgatt gctgtttttc tgttggggct cggttgttta ttgtgcacaa
    12301 aactgggaga tattcgaaaa ttttttatat acgttttact gtttatgact tcaggggcgg
    12361 tgcttctatt ttcggccttt actatttttc cggcgcttac gacctgggcg gatctgaact
    12421 tgattttaaa agggttgact gtaaagtttg ggattttacc ccttcttttt tctcactatg
    12481 actcggtatt gaattggatt tttggtttag ggccaggaca taccgttggc aagttggctt
    12541 tcatgttgcc tcagtatgag gaacagcttc gttctttaag tgtgtcgttt tcatctgtga
    12601 ctcaacatgc atggaactat cagcagggaa attatctttc ccattccgtt acagggtcga
    12661 gcatgtggtc tcctttcttc ttctgggccg ggctatgggg tgatcttggg attttgggtg
    12721 ttgtatccta cggactgctt tggggcattg taattttgcg catctgtacg aacgatcttg
    12781 ctcgttttct cgccttcaca gttctaattt ttgggatcgt cttttcctgg ttagaagaac
    12841 ccggctatat gctatttgtg gtgaccatta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn ngttttgtta gggttggagg gggggcaggg ggatagatgc aatccattgc
    13021 cagattcgtc gcaagcgagt ccggatgtat aaaaaatgag ttttgatcct gctttgcagt
    13081 tgattgccat tcagggaaac gtatactttt ccctccttcc cccagtcgaa atgctcctgg
    13141 atgtgagggc ttcatccata tgtttccatc tgaaaataag ccattcagga atatgaaatg
    13201 cggatctgta atcaacaagg catgatcgcc tgaaatgata ttattggtta attgccatcg
    13261 ctctgttttg tgctctattc tttcccctgt ttcccaattg ttgaccattc gggtaaacgg
    13321 agcctgcaca ataagttgat acccccggtt ggcataaatc cggcttccag ttaatgtgag
    13381 gtcaggtgtt tgcacggttc gaaatcccca ctgattattc tggtaaattt tggcgttggt
    13441 tatggtgatc cgttcactgg cttcgacgaa taatccgtac gttcggtttc cacaccaacg
    13501 gccgtcttcc atccgaatat ttcgattgtc atagtctaac caaaatcccg tggtgtggtt
    13561 cccttgtgcc atgtgccgtc taaaaatggc atcatgaatt ttcaagcctt taaagccagc
    13621 cactccccag ttaacgaact ccccttttac tcccctccaa ttattgcgta acgctttgtc
    13681 atcctcagat aggaggttct tgatcgtaga gatctgatac cctgaagcac cattgttgct
    13741 ggtctcattt cgtttgcttg ttaggttttg gatgttgtgc aaaaaatacc ccatgccatt
    13801 attccaggaa aagagaagat cctctaacgt aacattgttt ccatctgtaa tgactaaagc
    13861 gctaccgaga taatcagagg cggcatgctg aaaggttaag cctttgagaa caagatttgt
    13921 ttttcctttt ataagtactg gacggctacg aatggcgact tcaattaaac tttcagaagg
    13981 ggctaatcct tcaggtaggg caatgaaaag ctggctggtg ctttctgtcg caaaaaatgt
    14041 cccagctact aattcaattg ccgacaagac ctgttttaat ggaattccat tgacgaaaac
    14101 catttccttt cttctcacaa tatgacttac gttgacccta cctttccaac tatctggaag
    14161 tggtttcact ccccacttat agggccaggc atgaacgaag atattgggaa tttttgtagg
    14221 tttccagttt cgaaaaacat ctgctccgga aatgatagct ttgccaattt cggtcgcctc
    14281 aaagacaatt ggaggatctg aatcctgtgg attgacgatt aattggaatt tttcccggta
    14341 gacaccagga gccacaagaa tttttgtgcc aagtccaagc tcacgatttt caatggctct
    14401 tgttgtggcc tgtcccaagg ttttgagtgg caagtcggca gttcccggat tagaatcaga
    14461 cgcaacgagg tgtttattgt tgacatagat ggttcgatct acctctatga gccctaaggg
    14521 gagcggggct ccataggaac tagcttgcaa tatattcggt aagcctaaga cgaataatat
    14581 tgaacacaat ttcacatagt tcagaacgtt
//
LOCUS       DAORAQ010000063        14315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_709_length_14315_cov_61.024334, whole genome shotgun sequence.
ACCESSION   DAORAQ010000063 DAORAQ010000000
VERSION     DAORAQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14315
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_14315_cov_61.024334"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PKM72_07575"
     CDS             complement(<1..962)
                     /locus_tag="PKM72_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNP60682.1"
                     /translation="MKLTRLKVLKSSVLLGVMLVGTPVMGEHDLFPPIDQQIPHYASQ
                     SDVSGKINIVGSNTMDPILKKWKDQLGKVQPHLVITLDTEGSKVGFESLMSGKTHMAA
                     MSRPLKNEEIELFTKRLGYAPTTVPVAVDAFAVFVHKDNPLDQITFQQLDAVFSSERR
                     RGAQESLDHWGQLGLSGSWKQAPILAQIRDSNSGTGQFFRELVLMGGKDKESVAVQPG
                     AASVVHAVMNDPYAIGYSGIGYRTDSVKPLRVATEEGESFVEPTFESASNGSYPLRRV
                     LYLYVNHSPQAQDSPLLSEIIKFAVSQEGQQVVAKSGFFPLPTK"
     gene            1347..1646
                     /locus_tag="PKM72_07580"
     CDS             1347..1646
                     /locus_tag="PKM72_07580"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNP60683.1"
                     /translation="MKTKITSPACAEKLKVLSDPTRLGVLESLMSGPKNVGELMGELD
                     VEQSLLSHHLAILRDAGLVESSREGKTMIYQLPECVADSTAGKAINLGCCKISFD"
     gene            1666..2511
                     /locus_tag="PKM72_07585"
     CDS             1666..2511
                     /locus_tag="PKM72_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNP60684.1"
                     /translation="MVFSSFFIFVLRVILISHFLWTFPPWEYPIARAEDTRLIISGSS
                     TMAPLVSEIGKRFETLHPGNRVDVQTGGSSRGIADAVTGLADIGMASRALKPQEAHLF
                     GSIIAHDGITIILHRNNPVKFLTDTQIRDMYTGTITNWKTAGGNDAPTLVVNKAEGRS
                     TLELFLQYFNLKNRDIHAHVVIGDNEQGIKTVAGNPHAIGYVSIGTAQYDATHGIPIR
                     LLPLQHIPATIETVRKGTFPLSRPLTLVTNSPPAGLIKTFIDFAQSPQNHDLILQQYF
                     APLQE"
     gene            2531..3355
                     /gene="pstC"
                     /locus_tag="PKM72_07590"
     CDS             2531..3355
                     /gene="pstC"
                     /locus_tag="PKM72_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNP60685.1"
                     /translation="MRCLALTAGGLLLLVIAFLFHESWPALHHIGLTRFFSDPDWYPT
                     DNSYRLTSMVWGTIFASTGAVLLASPLAILSALFCHFYAPPLLSRWFRRMVELLAGIP
                     SVVFGFWGLVVLVPMIGHWHPPGPSLLAGILILAFMIMPTIMLVAHTSIAHVPLAYIH
                     SASALGFSRWSMMWHVILPTTKVGIFTGILLGLARALGETMAVLMVCGNVVQTPSRLF
                     DPIRTLTANIALEMGYALGDHRAALFMSGLFLMGLVVLFVSMAEGLRHRNTYVATK"
     gene            3339..4193
                     /gene="pstA"
                     /locus_tag="PKM72_07595"
     CDS             3339..4193
                     /gene="pstA"
                     /locus_tag="PKM72_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNP60686.1"
                     /translation="MSRQSNHVQEYCLTVIIWTAATMVSIPFIWLMGNLLWNGWSHLS
                     FNFVFSAPLNAGREGGIGPILISTLLLVGVCMGVAVPMGLGTALFLSEYTRHDHALGR
                     LIRGSLDVLAGVPSIVFGLFGNALFCQVLGFGFSILSGGLTLACMVLPILIRTVEESF
                     RVISREQRLSAAALGLSKSTTIQTILLPAAIPGLLVGGILGLGRAIAETAALIFTSGY
                     VDRMPESLMDSGRSLAVHIYDLSMNVAGGNPHAYATALVLVSLLFIINLSASWIASQW
                     TLSNQLPT"
     gene            4163..5032
                     /locus_tag="PKM72_07600"
     CDS             4163..5032
                     /locus_tag="PKM72_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNP60687.1"
                     /translation="MDLIQPTSHIIPDPDKRAGFRAWLPYQSTEPCCEPDAHFRANNL
                     SIAYHDLQVLRNVTLEINRGCITALVGPSGCGKTSFLMSLNRLTDLIPGCQVSGHLHM
                     NELDVLAPTTDLLQLRRTVGMIFQKPTVFPFSIRKNLELPLREHGTHDRTLLQSAIEN
                     VLQQVGLWHEVKDRLDHPAQALSGGQQQRLCLARALVLTPDVLLLDEPCSALDPISSG
                     IIEDLIASLQGSYTILIVTHNLAQARRLADHVAVFWSLDGVGQLVEHGSAQQIFESPK
                     EELTAAYISGIRG"
     gene            5190..6563
                     /locus_tag="PKM72_07605"
     CDS             5190..6563
                     /locus_tag="PKM72_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNP60688.1"
                     /translation="MKVFTLLTGLVCLGIVLQTSAWASDELIDVLREKKVLTKEDWIR
                     IEAAEEKKAVEQQKALDAQFPVKVGWGSKGFEFSTIDGKFATQIQWRFQGRYTYPTSG
                     DPISAADFNAGPESTLELRRVRMKIGGHGYQPWIKYYFELDLQSTSNSGGSPGSTRLI
                     DWRISLEKFKFLSLQLGQWKVDYNRERRDSSGTQQFVERSIVNEIFTIDRQVGAMLYG
                     HVAPGTAFDSRYYAGVFTGSGRGEANDDANMMYFGRYQWNFLGRDLKWSQSDVEFHEQ
                     PTGSVAFGAYTTVGKCTRWSSSGCGTLPEDNSAGVPYTSDSNAKAGQYRVQGMVEEFA
                     FKWRGLSIQHEYHWKEVKDNSYPEGAPGHKTNMMGSYSQIGYFPHGLIPAVPKPLEVA
                     FRYAFVDPNVSTPNDRRQEYTTAINWFFAGHKNKITLDGSWLTLSQPAGQNQHEQRVR
                     LQWDVSF"
     gene            6891..7889
                     /locus_tag="PKM72_07610"
     CDS             6891..7889
                     /locus_tag="PKM72_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNP60689.1"
                     /translation="MKIPIHLVMATFALTITASISTVSARELVKIDGSSTVFPITEAM
                     AEEFQKAERGKTMVTVGISGTGGGFKKFCRGETDIADASRPIKESEIKSCKEHNIGYI
                     ELPIALDALTVVVNPKNDWADHLTVEELKTAWEPGAQDKVTNWNQIRSSFPDRPLKLF
                     GAGTDSGTYDYFVEAIVEGKSSRGDFTASEDDNVLVQGVSNDINAMGFFGLAYYEENK
                     GKLKAVPISWKGGKPVGPSVDNAKNATYQPLSRPIFIYVNKDAADKKPHVKRFIEFYL
                     NNENAQTLVREVGYVPLPSKAYTMALDKFTKRLTGTVFHGAEVGVGIEDLLARVPQ"
     gene            7973..8926
                     /gene="pstC"
                     /locus_tag="PKM72_07615"
     CDS             7973..8926
                     /gene="pstC"
                     /locus_tag="PKM72_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNP60690.1"
                     /translation="MPTLPPSSSLSASSGSPLRASKGFLRRRALWGSLAGGCLLIAAA
                     TSVFVTLGIVYVLVTESMGFFAQISLLDFLTDTQWTPVFENARFGVMTLVSATVTVAG
                     IALAVSIPAGLALAIYLSEYARREVRETVKPFLELLEGVPTVVYGYFTLLFFTPLLQT
                     FIPGLEGFNMLGPGIVMGIMILPYTASVSEDAMRAVPPGLREAGYALGYSRFRVAMRV
                     VVPAAFSGITAAFILGMSRAVGETMVVAIAAGQNPNFSFNPTEGAATITAYIVQMAMG
                     DLPHDSIAYQSVFAAGIVLFVITLTFNLIGFYLRRRFKEVY"
     gene            8926..9864
                     /gene="pstA"
                     /locus_tag="PKM72_07620"
     CDS             8926..9864
                     /gene="pstA"
                     /locus_tag="PKM72_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNP60691.1"
                     /translation="MIKADVSQAIIPDLARIVDANRRTDLVMAGIGISVLIVAMSLLA
                     AVVIDLIVGGWPRLMNPDFYLNFPSRHPLQAGILSAWVGTILVMLVTACIAIPLGVGA
                     GVYLEEYAARNWITDFIEINVSNLAGVPSIIYGLLALGLFIHGFGLGETILTAGLVLA
                     LLILPVIIVSTREAIRGIPLDLREAAYGLGADKWQTVRLYVLPAARAGILTGTIVGLA
                     RAIGEAAPVITIGALTFIAFLPAPPFQASPPFINFEWLESPFTIMPIQMFNWVSRPQA
                     EFHINAAAAGVILMLMTLAMNGVAIWLRYHLRRQVR"
     gene            9927..10760
                     /gene="pstB"
                     /locus_tag="PKM72_07625"
     CDS             9927..10760
                     /gene="pstB"
                     /locus_tag="PKM72_07625"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNP60692.1"
                     /translation="MTQKISHDQQVTLMSLNASPTTNTERESLAAEVRKLSFWYGETQ
                     ALKEISLSIANRKVTALIGPSGCGKSTLLRSFNRMHDLYPGNRYSGEIRLFPENHNLI
                     DLDPFMVRLRIGMVFQKPNPFPKSIFENVAAGLRIQGVQNKKLLSDRVEQALRDAALW
                     EEVKDRLSQSAYGLSGGQQQRLCIARALAPQPEILLLDEPTSALDPIATAKVEDLITE
                     IRHRFTVIIVTHNMQQAARISDFTAYMHLGKLIEFAPTGELFTTPQETLTRDYITGRF
                     G"
     gene            10793..11482
                     /gene="phoU"
                     /locus_tag="PKM72_07630"
     CDS             10793..11482
                     /gene="phoU"
                     /locus_tag="PKM72_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNP60693.1"
                     /translation="MNNQHTLRIFDEDLNTLRQEIVALSNHVSEQLHRALTALVENNA
                     DLAEQVIANDRIADALQEKINTHTTRTLARQQAMADDLRAILAASRIAIHLERIGDYA
                     KNTAKRSRHLARPISAEFAEEFLWMGKRIQSMLSQVTESYIHHNAPLANVAWTDDAEL
                     DTVYANLFEHLLRMMSQESRNIGDGTQLLFIAKGLERAGDHVTDIAEEIYLMVTGLPL
                     QSPRPKVSELL"
     gene            complement(11581..12315)
                     /locus_tag="PKM72_07635"
     CDS             complement(11581..12315)
                     /locus_tag="PKM72_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60694.1"
                     /translation="MKRSAGLMGFIVVGWIFLGGCQGPQFQTLKISDSPNRVVALQAM
                     PDAFGGKGYDHPVVFTKEEMMQIMQGIRAEKSGLYSASSSGSSRAHPVFSPSEIDFFA
                     PLIVKGLSQATPEEMVTFFETAEIETDDLEKYFQLTTSGGFYVAGGKLYVVVSNFSVK
                     TPLWQDAQKSSYDVNSVRTNALEQLKPQPGLLVFEPREFMSESSDGEDIGSFLKGKPW
                     HVAIGYREFLGHSLSPETPMNKRKAD"
     gene            12501..12884
                     /locus_tag="PKM72_07640"
     CDS             12501..12884
                     /locus_tag="PKM72_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP60695.1"
                     /translation="MVRVEQLMTRELACVEFTQAVSVAANLMRIRKIGSLLVKHGEEL
                     VGIVTESDIVRKVVAFHFPSEYIQVGNIMSSPIVSIDESESISEAAGIMKAAYTRHLV
                     VGNESHVSGMLSVRDLLAPVAKDEL"
     gene            12889..13416
                     /locus_tag="PKM72_07645"
     CDS             12889..13416
                     /locus_tag="PKM72_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNP60696.1"
                     /translation="MKATMQSNDSSMFNSSFQQSSAWLAKALEADLTHFWMKGIKAVL
                     SLLIVTILVGLAGGVLRIFLNLEALFTEPLEQVFRQLIVDTLIILAIVEVFKTTVTYF
                     SEGRVKVTFIVDTILVVMLTEIISKWFSEAHLEQWMILGGILVVLAIIRVVAVQWSPT
                     RGENTQEWPQAQKSH"
     gene            13699..13854
                     /locus_tag="PKM72_07650"
     CDS             13699..13854
                     /locus_tag="PKM72_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60697.1"
                     /translation="MKFMIMIVGVLILLQPLIYGIHTLLYLSRRPRHCYPILHGKGPK
                     GKFSLVA"
     gene            13927..14310
                     /locus_tag="PKM72_07655"
     CDS             13927..14310
                     /locus_tag="PKM72_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP60698.1"
                     /translation="MIRVEELMKQDLASVNCGDLVSLAATLMRIRRIGCVFVQQEGHI
                     VGIVTEADIVRKVVSTRRSPDQTTVETIMTAPVITIDQDAPIFEAADLMDRSGTRHLG
                     VTHREDIVGIVSVRDLLHPVAIDEF"
BASE COUNT         3458 a         3816 c         3434 g         3607 t
ORIGIN      
        1 tctttggtgg gtaacgggaa aaagcctgat ttggcaacaa cctgttggcc ttcctgactt
       61 accgcaaact tgataatctc cgacaggaga ggagaatcct gtgcttgtgg ggaatgattc
      121 acatacagat agagcacccg acgaagtgga taggatccat tgctcgcgga ttcaaatgtc
      181 ggctcgacaa aagactcccc ttcctcggta gccacacgca ggggtttgac ggaatcggtg
      241 cgatagccga ttccactata ccctatggca taaggatcat tcataacggc atggacaacc
      301 gacgcggcgc ctggttgaac cgcaaccgac tctttatctt ttccgcccat caagactaat
      361 tctctaaaaa actgtccggt tcctgaattg gaatcccgaa tttgcgcaag gataggcgct
      421 tgtttccagc ttccggaaag gcccaactgg ccccaatggt ccagagactc ctgagcccct
      481 cgtcgccttt cggaggaaaa cacagcatct aattgttgaa aggtgatttg gtcgagcggg
      541 ttatccttat gcacaaagac ggcaaatgca tccacggcaa cgggtaccgt agtgggtgcg
      601 taccctagcc gtttggtgaa tagctcgatt tcctcatttt tcagcggacg ggacatggcc
      661 gccatgtggg tcttcccact cattaatgac tcaaatccga ctttggaacc ctccgtgtcc
      721 agggtaatga ccaaatgagg ttgaactttt cccaactggt ctttccattt cttcagaatt
      781 gggtccatgg tattggagcc cactatgttg atcttccctg atacgtcact ttgtgaagcg
      841 taatgaggaa tctgttgatc gataggggga aacagatcgt gttctcccat aacaggtgtc
      901 ccaaccaaca ttacgcccaa caaaacactg gacttaagga ccttgagtct tgtcaatttc
      961 atgttcgccc ctcgtagtaa ggattaatat gccttattgt ctctcatgcc accattttca
     1021 caaaaattga aagaagcctt ccctcattct cacattttct attattgagg atagagccta
     1081 cctgcgtatt gtgtcggtat tatgtctcgc atgttacaaa tacgttaact tttatttgag
     1141 gactccctgc tggaatttat cgttttgagt gtataaatcg gaggtacctt cgttagaaaa
     1201 cctaatttat ccaaactatc caaaatactg cacgactgca tccatgaaac cttgactcac
     1261 aataatggtc catattaaat tctattcata tgaatacata atcatattaa atagattttt
     1321 acattcaaaa attttcattc gaccccatga aaacaaagat tacatccccc gcttgtgcgg
     1381 aaaaattgaa agtgttatcg gacccaaccc gcctcggcgt gcttgaatca ctcatgtcag
     1441 ggccgaaaaa tgtcggagaa ctcatgggtg agttggatgt tgaacagagt ttattgtccc
     1501 accatttggc catccttcgc gacgcaggct tagtggagtc ctcgcgggaa gggaaaacca
     1561 tgatttacca attgccggaa tgcgtcgcag attctacggc gggcaaggcc atcaatctgg
     1621 ggtgctgcaa aatttccttc gattaacgcg tcgttcttta cccttatggt tttttcgtct
     1681 ttttttattt tcgtactaag agttatcctg attagccatt ttttatggac attcccgccg
     1741 tgggaatatc cgatcgcccg ggcagaagac acaagactga tcatctccgg ctccagcacc
     1801 atggctccgt tggtatccga aatcggcaaa cgctttgaga ccctccatcc aggtaatcgg
     1861 gtggacgttc aaaccggagg ctcctctcgc ggcattgcag acgctgtgac cggcctggcc
     1921 gatatcggca tggcctcccg ggctctgaaa cctcaggaag ctcatctatt tggatccatc
     1981 attgcccatg acggcattac catcatcctc caccgtaata atcccgtaaa gtttttgacg
     2041 gatactcaga tcagagacat gtacaccgga acaatcacaa attggaaaac agccggcgga
     2101 aatgatgcac ctaccttggt cgtcaataaa gccgaagggc gctcgacact cgaactgttt
     2161 cttcagtatt tcaatctcaa gaatcgggac atccacgccc acgtcgtgat tggggataac
     2221 gaacaaggca ttaagacagt agccggcaac cctcacgcta tcggatatgt ctccattggc
     2281 acggcccagt atgatgcgac ccatggcata cccatccgat tgttaccgct gcaacacatt
     2341 ccggccacga tcgagacagt caggaaaggc acgtttcccc tctctcgtcc actaaccctt
     2401 gtcacaaatt ctcctcccgc aggactcatt aaaaccttta ttgatttcgc gcagtcacct
     2461 caaaaccatg atctcatcct ccaacaatat ttcgccccac tccaagaatg atgatctgct
     2521 ctcagggagt gtgagatgtc ttgcgttgac ggcaggagga ctcctgcttc tggtcatcgc
     2581 atttctcttt catgaatcct ggcccgccct tcaccatatt gggctcacac gttttttcag
     2641 cgatcctgac tggtatccta ccgataactc gtatcgcctg acttctatgg tctggggaac
     2701 catcttcgcg tccaccggtg ccgttcttct tgcctccccg ctcgcaattc tctcagcgct
     2761 tttctgtcat ttttatgcgc cccctcttct tagccgatgg ttccgccgca tggtcgaact
     2821 cctggccggg attccttcgg tcgtcttcgg cttttggggt ctcgtggttc tggttccgat
     2881 gatcggccac tggcatcctc ccggcccaag cctgcttgcc ggcattctca tcctggcgtt
     2941 tatgatcatg cccaccatca tgttagtggc ccataccagc attgctcatg ttcccctcgc
     3001 ctatatccat agcgcctccg ccttaggatt cagtcgatgg tcgatgatgt ggcatgtgat
     3061 tcttcccaca accaaggttg gcatttttac cggcatccta ttgggattgg cacgtgccct
     3121 cggagaaacc atggccgttc taatggtctg tggaaacgtc gtacaaactc cttcccgtct
     3181 gtttgatccg atcagaaccc tgaccgccaa cattgccctg gaaatggggt atgcgttggg
     3241 cgatcatcgg gcggcattgt tcatgagtgg actcttcctc atgggactcg ttgtgctatt
     3301 tgtcagcatg gcagagggct tacgccatcg gaacacctat gtcgcgacaa agtaaccacg
     3361 ttcaggaata ctgcctcacc gtgatcatat ggacggcggc caccatggtc tccattccgt
     3421 ttatctggtt aatgggtaat ttactctgga atggatggag ccacctgtca ttcaacttcg
     3481 tgttctctgc tcccctgaat gccgggcggg aaggcgggat cggccccatc ctcatatcca
     3541 ccttgctact cgtgggagta tgcatgggag tggccgtgcc tatgggtttg ggaacagcat
     3601 tgtttctttc ggaatacacc cgtcacgatc atgcgctggg ccgactcatt cgtgggtcac
     3661 tggatgtcct cgcaggtgtg ccctccattg tctttggcct gtttggcaat gccctgtttt
     3721 gccaagtcct gggattcgga ttttccattc tgtcgggagg tctcacattg gcctgcatgg
     3781 tgttacctat cctgattcgc acggtggaag aaagttttcg ggttatttcg cgtgaacagc
     3841 gattatccgc agcggccctt ggcctttcaa agtcaaccac tattcaaacg attctcctcc
     3901 cggcggccat ccccggactg ttggtcggcg gcatccttgg gctgggacgg gcaatagccg
     3961 aaactgcggc cttaattttt accagtgggt acgtggaccg aatgccggag tctttaatgg
     4021 attcaggacg atcgctcgcg gtgcacattt atgatctgtc catgaatgtg gccgggggga
     4081 acccccatgc ctatgccacg gctcttgtcc tggtgagcct cttgttcata attaaccttt
     4141 ctgcttcctg gattgcctca caatggacct tatccaacca acttcccaca taattcctga
     4201 tcccgacaaa cgggccgggt tcagagcatg gctcccctat caatccaccg aaccctgctg
     4261 tgagcctgac gctcattttc gcgcgaacaa cctttcgatc gcctatcacg atcttcaagt
     4321 ccttcggaat gtcactctgg aaattaatcg ggggtgtatt accgcgttag tggggccgtc
     4381 aggttgtgga aaaacgagct ttctcatgag cctgaatcgc ctgaccgatt tgattcccgg
     4441 atgccaggtc tcggggcacc tgcacatgaa tgaattggat gtcctcgccc ccacaactga
     4501 tctccttcag ttgcgccgta ccgtgggaat gattttccaa aaacccaccg tttttccttt
     4561 ttccattcga aaaaatctgg aactccctct ccgtgaacac ggaacgcatg accgcacact
     4621 attgcaatcg gccattgaaa acgtcctgca gcaagttggt ctctggcatg aagtcaaaga
     4681 ccggctcgac catcccgctc aagccctctc aggaggacaa caacaaaggc tctgcctcgc
     4741 aagagcactc gtattgacac cggatgtcct tctacttgat gagccctgca gtgccttaga
     4801 tccaatctca agcggaatca tcgaagattt aattgccagc ttgcagggtt cgtacaccat
     4861 cctcatcgtc acacataacc tagcccaagc ccgccgatta gccgatcatg tggccgtttt
     4921 ctggtcattg gatggagtcg gacaattggt tgaacatggt tccgctcagc agatatttga
     4981 atccccaaaa gaggaattaa ccgccgccta catttccgga attcgcggtt agatcagact
     5041 ctcttctccc cggatccccc cgtttttaca tcggtttgac cttctgttaa cagaatcgta
     5101 atccctgtgg gctagtctct ttctcaagat gcacgcacat tcttctcgtt taccacacat
     5161 agtttatcaa tcaaagggag gccgtattca tgaaggtttt tacattacta acgggactcg
     5221 tctgcctggg catcgtgttg cagacaagtg cctgggcgag tgatgaactg atcgatgtct
     5281 tgcgtgagaa gaaggtgctc accaaagaag attggatcag aattgaggcg gcagaagaga
     5341 aaaaagcggt ggaacaacaa aaagcgcttg atgctcaatt tccagtcaaa gtcggctggg
     5401 gatcgaaagg ctttgaattc tcaacaatcg atggaaaatt tgcgacacaa attcagtggc
     5461 ggttccaagg gcgctatacg tatcccacaa gcggagatcc gatatccgcc gctgatttca
     5521 atgcgggtcc cgagagtacc cttgaacttc gccgcgttcg aatgaaaatc ggtggtcatg
     5581 gctatcaacc ctggatcaag tactatttcg agttagatct gcaatccact tccaattccg
     5641 gcgggtcacc tggatcgact agattaatcg actggcggat cagtctggaa aagttcaagt
     5701 ttctttctct gcaacttgga caatggaaag tcgactataa ccgcgaacga agagactctt
     5761 ccggcaccca gcagttcgtg gaacgatcaa tcgtcaatga aatctttacc atcgaccgcc
     5821 aggttggggc catgctgtat ggccatgtgg cccctggtac ggcctttgac tcacgatatt
     5881 atgccggtgt ctttacagga tccggtcggg gagaagccaa cgacgacgcc aacatgatgt
     5941 acttcggacg gtatcaatgg aatttcctcg gacgcgatct gaaatggtca caaagcgacg
     6001 tggagtttca cgagcagccc acgggaagcg tggccttcgg ggcctatacc actgtcggca
     6061 aatgcaccag atggtcctcg agtggttgcg gaacattgcc ggaagacaat tcggccgggg
     6121 taccctacac ctcggactcc aacgcgaaag ccggacaata ccgggtgcag ggcatggtgg
     6181 aggaattcgc gttcaaatgg cgggggctat cgattcaaca tgaataccat tggaaagagg
     6241 ttaaggacaa cagctatcca gagggtgcgc cgggccataa gaccaacatg atgggaagtt
     6301 actcacaaat cggttatttc ccccatggtc tcattccggc agtccccaaa cccctggagg
     6361 tcgcctttcg ttatgcattt gtcgacccca acgtctcaac cccaaatgat cgacggcagg
     6421 aatacaccac tgccatcaac tggtttttcg caggacataa aaacaaaatt actctcgatg
     6481 ggtcctggct cacgctgtcc caacccgcag ggcaaaatca gcatgaacag cgggtccgtt
     6541 tgcaatggga tgtgtcattc taaatgtatc gggacgtaaa ggtactcctc acgtcccacg
     6601 aacaggcctc tatcggctgt catccgaaga ggcctgttct tccattgggt acgccaacgg
     6661 aatggaagac ccggcgggat ctccctgaat cagccttcta gaaggtacat gggaaaaagt
     6721 tgagatcgaa taaatggcat gtcttccgcc agtaacattt aacctgatgg taatgagaat
     6781 gtcatggacg tgtcatctta tcctgagata gtcggatgat tcatctttca agaaattatt
     6841 tcatactact ccaattacca ttctcacact taatcatacg gaggtttcgc atgaaaatcc
     6901 caatccacct tgttatggcg accttcgctc tgaccatcac cgcgtcgatt tccaccgtgt
     6961 ccgcccgcga gttggtcaaa attgacggct caagcacggt ctttccgatt accgaggcga
     7021 tggctgaaga atttcaaaaa gccgaacgag ggaaaacaat ggtgacagta ggcatttccg
     7081 gcacaggcgg cggctttaaa aaattctgtc gaggtgaaac ggatatcgcc gacgcttccc
     7141 gtccgataaa ggagtcagaa attaaatcgt gcaaagaaca caacatcggc tatatcgagt
     7201 taccaatcgc cttggacgct ttgacggtcg tcgtgaatcc taaaaacgac tgggcggatc
     7261 atttgaccgt ggaggaattg aaaaccgcct gggaacctgg agcccaggac aaagtgacga
     7321 attggaatca aattcgaagc agcttcccgg atcgaccctt gaaactgttc ggggctggga
     7381 ctgattccgg gacctacgat tattttgttg aggccattgt ggagggaaaa agttctcgcg
     7441 gggattttac cgctagtgag gatgacaacg tcctggttca aggagtcagc aatgacatta
     7501 atgccatggg atttttcggc ttggcctatt acgaggaaaa taagggaaaa ttaaaagccg
     7561 taccgatttc atggaaaggc gggaaacccg tgggcccttc tgtggataat gccaaaaatg
     7621 ccacgtacca acccctatcg cgtcccattt ttatttacgt caataaggat gccgccgaca
     7681 aaaaaccaca cgtcaaacga ttcatcgagt tttatttgaa caacgagaat gcccaaacgc
     7741 tcgtgaggga agtcggatac gtgccgttgc cctcaaaagc ctacacgatg gctctggaca
     7801 agtttaccaa acgactcacg gggacggttt tccatggggc tgaagtcggc gtcggaatcg
     7861 aagacctgct ggccagggtt cctcagtaga gggaacctgg acaatcggat cggtcctctc
     7921 caggggtggc cagccgcccc tggaacatag aattttatct ggagttgaaa gcatgccaac
     7981 gttaccacct tcctcaagcc tctctgcctc ttcaggatct cccttacgcg ccagcaaagg
     8041 gttcctccgc cgccgtgcct tatggggctc tttagccggg gggtgccttt tgatcgcagc
     8101 cgcgacttcc gtgtttgtca cgttaggaat cgtgtatgtc ctggtcacgg agtccatggg
     8161 gttctttgcc cagatttctc tcctggactt tctgacagac acccaatgga ctcccgtttt
     8221 tgagaatgcc cgcttcgggg tgatgactct ggtctcggcg acggtcaccg tagcagggat
     8281 tgcgctagcc gtctctattc cggcgggact ggccttggct atttacctca gcgaatatgc
     8341 tcgaagggag gttcgcgaga cggtcaaacc ctttcttgag ttgctcgaag gggtgccgac
     8401 agtggtctac ggatatttca cccttctctt tttcaccccg cttctgcaaa ccttcatccc
     8461 cggcttggaa gggtttaaca tgctcggtcc cggcatcgtc atgggaatca tgattcttcc
     8521 ctacacagcc tcggtcagcg aagatgccat gcgggcagtc ccccctggtc tgcgggaagc
     8581 gggatacgcc ttgggatact cccgcttccg ggttgccatg cgggtcgtgg tccctgccgc
     8641 attttccggc atcacggcgg catttattct tggcatgtca cgagccgtgg gcgaaacgat
     8701 ggtcgtcgcc atcgctgccg gccaaaaccc caatttttct ttcaatccca cggaaggagc
     8761 agcaacgatc acggcctata tcgtacaaat ggccatggga gatttgcctc atgactccat
     8821 tgcctaccaa tccgtgtttg cggctggaat cgtgttgttt gtcatcacgc tcaccttcaa
     8881 tttgatcggt ttctatctcc ggcggcgctt taaggaggtg tactaatgat taaagccgac
     8941 gtgtcccaag cgattattcc cgatttggcc cgtattgttg atgccaaccg ccgaaccgac
     9001 ctggtcatgg cgggcatcgg catttcggta ctgatcgtcg ccatgagctt attggcggca
     9061 gtggtcatcg acctgatcgt gggcggatgg cccagactca tgaaccccga tttttatctc
     9121 aacttcccct cgcgccatcc tcttcaagca gggatccttt ccgcttgggt cggcacaatc
     9181 ctggtcatgc tcgtcactgc ctgcattgcg ataccgctgg gggttggagc aggggtgtac
     9241 ttggaggaat atgctgcgcg caactggatc acggatttca ttgaaatcaa cgtcagcaat
     9301 ctagccgggg tgccttccat tatctacggg ttgttagcgc ttggtctgtt catccatggg
     9361 ttcgggctcg gagagacgat cctcacggct ggcctggtac tcgcattact gattttgccg
     9421 gtaattattg tatccacccg tgaagccatt cgtggaattc cccttgattt gcgggaagcc
     9481 gcatatgggt tgggtgctga caagtggcag accgttcgcc tgtacgtgct gcccgctgcg
     9541 cgggctggca ttttgaccgg aaccatcgtg ggtttggctc gagcgatagg agaagccgca
     9601 ccggtcatca ccatcggggc cttaaccttt atcgcctttt tacccgcccc cccttttcag
     9661 gccagcccgc cttttatcaa ttttgaatgg ctcgaaagcc catttaccat catgcctatt
     9721 cagatgttca attgggtctc acgaccccag gcagaattcc atattaatgc cgcggcggcc
     9781 ggagtcattc tgatgctgat gacattggcg atgaatggag tagccatttg gttgcgctat
     9841 catttacgcc gccaggttcg ttagccatcc atgatgtccc agatgaatat tggatgccct
     9901 cttagccgaa ccaaggaacc acgcatatga cacagaaaat ttctcatgac cagcaagtca
     9961 cactcatgtc gctcaacgcc tcccccacaa ccaacaccga acgggaatcg ctggcggcgg
    10021 aagttcggaa actcagtttt tggtatggtg aaacccaagc cttgaaggaa atctccttat
    10081 caattgccaa tcggaaagtc accgcattga tcggtccatc cgggtgtggg aaaagcaccc
    10141 ttcttcgatc gtttaatcgt atgcatgatc tgtatcccgg aaatcggtat agcggagaga
    10201 ttcgtctttt ccctgaaaac cacaacctca tcgaccttga tcccttcatg gtcagacttc
    10261 gaatcgggat ggtgttccaa aaacccaatc cttttcccaa atccatcttt gaaaatgtag
    10321 cggccggcct tcggattcag ggcgttcaga acaaaaaact gttgagcgac cgtgtggaac
    10381 aggccttgcg ggatgcggcc ttgtgggagg aagtgaagga tcgcctttca caatcggcct
    10441 acggcctctc aggcggacag cagcaacgat tgtgtatagc cagggccctg gccccacaac
    10501 ctgaaatcct cttactggat gaacccacct cggcattgga ccccattgcc accgccaaag
    10561 ttgaagatct gattacagaa attcgccatc ggttcacggt gatcattgtc acccacaaca
    10621 tgcagcaggc ggcccggatt tcggacttta ccgcctacat gcatctgggc aaactcattg
    10681 aattcgctcc aacaggagaa ctgtttacca cgccgcaaga aacgctgacg cgggattata
    10741 tcaccggccg gtttggataa accggcggaa caccgatttc gaaaggatca tcatgaacaa
    10801 tcaacacacc ctacgaattt ttgacgaaga tttgaacacc cttcgacagg agattgtggc
    10861 tctctcaaat cacgtgtcgg agcaactgca ccgtgcgctg accgccttgg tggagaacaa
    10921 tgccgacttg gcggagcagg ttattgccaa cgaccgtatt gcagatgccc tgcaagaaaa
    10981 aatcaatacc catacgaccc gcactctggc ccgtcaacag gcgatggcgg acgacctgcg
    11041 agctattctg gcggcttctc gcatcgcgat tcatttggaa cggatcggtg attacgcaaa
    11101 aaataccgcc aaacgcagcc ggcatttggc acggcccatc tctgccgaat ttgctgaaga
    11161 atttctatgg atggggaaac gcatccaatc catgttgtcc caggtcacgg agtcctatat
    11221 tcaccataat gccccgttgg ccaatgttgc ttggacggat gacgccgaat tggacaccgt
    11281 ctatgccaac ctgtttgagc acctgctccg catgatgagc caggaatcca ggaacatcgg
    11341 tgacgggact caattattat ttatcgccaa agggttggaa cgcgccggcg atcatgtgac
    11401 cgacattgct gaagaaatat atttaatggt cactggcctt cctttgcaaa gcccccgtcc
    11461 caaagtcagt gaactgttat aaagaattag acgcacacca cattcaaatc ccctcgagac
    11521 ctcagaatta tagagaaggc ttccgtcatc gcagagggac gtcaggttgg aaggaagatc
    11581 ttaatcggct tttcgtttgt tcatcggtgt ttcaggagag agtgaatggc ctaaaaactc
    11641 acgatacccg atggccacat gccagggttt tcctttcaag aaacttccga tgtcttcccc
    11701 atcggacgac tccgacatga actctcgtgg ctcaaacacg agcagaccgg gctgaggttt
    11761 taattgctca agagcattgg tgcgaacaga gtttacgtca taactcgact tctgcgcatc
    11821 ctgccagagc ggagttttca cggaaaagtt actcacgaca acatacagtt ttcccccagc
    11881 gacatagaac ccacccgagg tggtgagttg aaaatacttt tctagatcgt ccgtttcaat
    11941 ttcggcagtt tcaaagaagg tcaccatttc ctccggagtg gcttgactca accctttgac
    12001 aatcaagggt gcaaaaaaat caatctctga tgggctaaaa accggatgtg cccgggaact
    12061 accggaggat gaagcgctgt aaagaccgct tttttctgca cggattcctt gcatgatttg
    12121 catcatttct tctttcgtga acaccaccgg gtgatcatag ccttttccgc caaaggcatc
    12181 cggcatagcc tgtaaggcca ccacacgatt gggactatct gaaattttca atgtttgaaa
    12241 ttgtggcccc tgacatcctc ccagaaaaat ccatcccacg actataaacc ccatcaaccc
    12301 tgcagatcgt ttcatacaga tattcctccc atcaaccatg atcatacccc tgcttttccg
    12361 agtcattcta ccctagacgt ccaccacaaa aggagtggag gagaaaaaat gttacacgta
    12421 cgtcatatac ttgcgacgta ggtttaatac caatccagta cattccatca tgaaccttca
    12481 tattaacaaa ggagagaaat atggtacgag ttgaacagtt aatgaccaga gaactggcct
    12541 gtgttgagtt cacccaagcg gtatcagtcg cggccaacct gatgcgaatt cgcaaaattg
    12601 gcagcctgtt agtgaaacac ggagaagagt tagtgggaat tgtgacggaa agcgatatcg
    12661 tgagaaaggt cgtggcattt catttccctt cagagtatat ccaagtcgga aatatcatga
    12721 gcagtcccat tgtctccatt gatgaatcag agtcgatttc tgaggctgcc gggatcatga
    12781 aggcggctta cacgagacat ttggtggtag gcaatgagtc ccatgtgtcc ggcatgcttt
    12841 ctgttcgtga tttattagct cctgttgcaa aggatgaatt ataaaaccat gaaagccacg
    12901 atgcagtcga acgattcctc catgttcaac agctcctttc aacagtcatc cgcctggctg
    12961 gccaaagcgt tagaggccga cctgacccat ttctggatga aaggtatcaa agccgtcctg
    13021 agcttattaa ttgtcaccat cctcgtagga ctcgctggag gcgtcttgag gattttcttg
    13081 aatttagaag ctcttttcac agaacccctc gaacaggtct ttcgtcaact catcgtggac
    13141 acgttgatca tactggccat cgtggaggtc ttcaaaacaa ccgtgaccta tttttccgaa
    13201 gggcgggtga aggtcacgtt tattgtcgat acgattctcg tggtcatgct taccgaaatt
    13261 atctcgaaat ggttttcgga agcccatctg gaacagtgga tgatcttggg tggaatatta
    13321 gtggtattgg ccatcatccg ggtcgtggcc gtccaatggt ctccgacgcg aggcgaaaat
    13381 acccaggaat ggcctcaagc tcaaaaatct cactaacctc gatcgagttg gagggcaatt
    13441 cccaacactt cccggcggat tgaaaatttg actcatcact tttcaatcgg ccacatccct
    13501 gaaatatttt gtaaagaaac ggaaaatgtg acgggttccc ccccctgaat tcctaaaatc
    13561 caattgcacc agttcaccac aacatcatga aatgttacga acagctaacg attcggaaac
    13621 cccatggtaa caccaccatg tcatgctact cagagcatca agaaacgatg acacaaccaa
    13681 tccagcagga gacacacaat gaaattcatg atcatgatag tgggagtact catactcctt
    13741 cagccactca tttacggtat tcatactctt ctctatctct cccggcgccc acgccactgc
    13801 tatccgattt tgcatgggaa aggcccgaaa ggaaaatttt cgcttgtggc ttagacgccg
    13861 acttcaacca ggacgagagg attcggaact ctcaatctat aattcactaa caaaaggagc
    13921 ccaactatga ttcgagtcga agaactcatg aaacaggatc ttgcctcagt gaattgcgga
    13981 gacctcgtgt ccctagcagc taccctaatg cggattcgcc gcatcggttg cgtgtttgtc
    14041 caacaagagg ggcatattgt gggcatcgtc actgaagctg atattgtccg gaaagtggtc
    14101 tcgacccgtc ggtcgccaga ccaaaccacc gtcgaaacca ttatgactgc ccccgtgatc
    14161 accattgatc aagacgcacc gattttcgaa gcggcggact tgatggatcg atccggcact
    14221 cggcacctgg gcgtcactca ccgggaggac attgtcggga tcgtgtcagt tcgcgacctc
    14281 ctgcaccctg tggccatcga tgaattctaa tattt
//
LOCUS       DAORAQ010000064        13863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_738_length_13863_cov_64.889050, whole genome shotgun sequence.
ACCESSION   DAORAQ010000064 DAORAQ010000000
VERSION     DAORAQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13863
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_738_length_13863_cov_64.889050"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKM72_07660"
     CDS             complement(<1..661)
                     /locus_tag="PKM72_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNP60699.1"
                     /translation="MSNLNKVDEQYLALAVDLLETPSFAAKVTNLIGGPVERAITMLP
                     EGFSNKLLTTTQKALNKALNLAVSSINERYRGKPANGAHRVLTTVSGAIGGSMGLMAL
                     SVELPISTTLMLRSIADIARSEGEPIMEAGSKLACLEVFAMGGTSQADDGAETGYFAV
                     RAALAGAMTEAGKYLLERGLAGSGAPPLVRLLSEIASRYSIQVSEKAAAQAIPLLGAA
                     GG"
     gene            complement(771..1871)
                     /gene="ribD"
                     /locus_tag="PKM72_07665"
     CDS             complement(771..1871)
                     /gene="ribD"
                     /locus_tag="PKM72_07665"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNP60700.1"
                     /translation="MKRALSLAAKGKGRTSPNPMVGAVIVKDGQIVGEAYHRQSGEPH
                     AEVLALHQAGPRARGGVLYVTLEPCCHTHKRTPPCVPLLIQSALARICVAMIDPNPQV
                     NGRGLQQLKHANIPVSVGMLEQDARNLNEVYAYWITTGRPLVTLKGAMTLDGKLATAT
                     GESRWITGERARQDVHRLRNRMDAILVGVGTVIADDPELSARGKTMTSGRVGRQPVRV
                     ILDSRLRIPSNAKVLQWVEEQPTILCTTTQAPVKKIEHFEKRGIQVWVLPRQAGRVSL
                     KACLARLGKQGITSVLIEGGSKVNATALHQGLVNRVRLYTAPMLLGGQDAIGLIGGLS
                     PKKIDRAWPLADCQLKKLGKDWLMTGTIEPRR"
     gene            complement(1898..5014)
                     /gene="ppdK"
                     /locus_tag="PKM72_07670"
     CDS             complement(1898..5014)
                     /gene="ppdK"
                     /locus_tag="PKM72_07670"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNP60701.1"
                     /translation="MRHKKYVYFFGGGKAEGSGNMKELLGGKGSGLAEMTNLKISVPS
                     GFTITTEACVEYFYSKKRFPAGMWDQALNGLRQVEKTMKARLGDPDNPLLVSVRSGAR
                     ASMPGMMDTVLNLGLNQQTVQGLGKKTGNQRFAVDAYRRFITMFGSVVMGIPRERFEH
                     SLEAMKQAHGVKHDTDLSEQALAELVVQYQELVKTETARDFPQDPFEQLRMAVDAVFD
                     SWFGDRAVTYRRLYDIPDEWGTAVNVQSMVFGNMGDTSGTGVAFTRNPANGVPVFFGE
                     CLLNAQGEDVVAGIRTPMPVLALQDTLPGAYKDLIKTQRTLEKHYRDMLDLEFTIQEG
                     KLYMLQTRVGKRTGIAAVRIAVDMVRQGLIDRREAVKRVAPEQLSQYLYPIFESSDEA
                     KFQSVGKGLPAGPGAAAGHIALTPDQAVAMKAKGERAILVRHETSPDDIQGMHAAEGF
                     LTAKGGMTSHAAVVARQMGKVCIAGCDAVEVLPNGKVQFGSMVLSEGDFLSLNGFTGQ
                     VYAGDIPVVDSEVIQVIQGRMDAKDSEKYQYFSTILKWADAFRVLKIRSNADIPEQAH
                     IARGFGAEGIGLCRTEHMFFAEDRVPIMQQMILASSREERELYLEQLLPLQRQDFIGL
                     YREMKGFPVTIRLLDPPLHEFLPKREHLLVEIAKLEVTNGDPQVIQEKQRILDRVEEL
                     HEYNPMLGLRGCRLGITMPEITRMQVQGIFEAACEVAREGKKIVPEIMIPLVGMPSEM
                     KAQKELIQDVAEKTMAEYGMKLSYLIGTMIELPRAAVTAGRIAEHAEFFSFGTNDLTQ
                     TTFGFSRDDAGPYIGFYLDRQDRCPVCQRTNVDWKKMVCRMCKATIDKKAENILEADP
                     FAVLDQEGVGALMKWAIQEGRKTRPLIKLGICGEHGGEPSSVAFCHELGLNYVSCSPY
                     RVAIARLAAAQAAVAVLEREESQRESRSTFRIINKSRAVSSKSGSAKTPKKSPTKKTT
                     GKKVTTRKVVAGKKPATKKSAPKKPAAKSTVKKNVVTKTTAKKSTSKKKTSPSPTRKK
                     PTAAAKRR"
     gene            complement(5236..5721)
                     /locus_tag="PKM72_07675"
     CDS             complement(5236..5721)
                     /locus_tag="PKM72_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60702.1"
                     /translation="MGGSTDGGVSPKISRRLKRFTLVVTVALIIVCNALILTGLWVSG
                     VDLESIVSKSELFDPENGHCVQVTWLKVAGVDGLVKVCSEWLDFSDISGETHRLPEGK
                     TLAMGPDGNLYVPGNPAENYRLIALMIFAIVVIISGMWTKQILIAKYQDRLQSVNHHS
                     I"
     gene            complement(5788..7977)
                     /gene="glyS"
                     /locus_tag="PKM72_07680"
     CDS             complement(5788..7977)
                     /gene="glyS"
                     /locus_tag="PKM72_07680"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNP60703.1"
                     /translation="MAPSRTTRKNVKVKSVPKSQPKTVPFLLEIGTEELPSAVIPQAL
                     EDLANLGQRLFADSRLEYESLRVVGTPRRLALLVKGVQPQQASLTQEILGPPVSAALD
                     ASGQPTKAALGFAKSQGMPVDRLRRKDTPKGAYMAVETHQRGQSAKRVITEALPQILS
                     KLAFPKAMRWNASRAKFGRPIRWVVALLGDQPLRIEFAGIQSGAMTKGHRFYRAKGKK
                     PGQAFPLTTANNYIEAMKRLGVLVDPEERRSLITEQVSALAKAAKGQVDPLYRDELIE
                     TAVFSVESPHAILGTFQKDFLAIPKPVLISSMKEHQGFFSLITKDGGLLPKFIAVTNM
                     PWGNTPLMTKGNERVLAARLSDAQYFFTEDSKRPLHERVPALEGVLLHHKLGTVRQKV
                     DRVGHLVGWMAEQIGGKDLDEMCRRAAFLAKADLTTGMVGEFPTLQGVMGEEYARHDG
                     ESPEVCRAIGEQYFPRFPDDQLPKELPGVLVAVADRCDSIVAFFAAGMSPSGSEDPLG
                     LRRAAYGLVRILAETPLRLNITSVVDQALRILDGQGGKVRGTPQTGSDVVAFILDRLR
                     FYGRQHLGLREDIMEAVIHVSNSMVCDMADLVARMQALEAMVPQPDFDPLMIGFKRAH
                     RIVEKEQWTNAQALPEHFVHESERRLFQALEVVQQQVRDSVNDRNYGKALLSLLELKS
                     PIDEFFGAVMVNDSDPQTRANRLSLLKATDDLFLTVADLSCLQSASG"
     gene            complement(8015..8932)
                     /locus_tag="PKM72_07685"
     CDS             complement(8015..8932)
                     /locus_tag="PKM72_07685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNP60704.1"
                     /translation="MTFQDIILSLHQFWKSHHCILTQPYDTEKGAGTFNPATFLRAIG
                     PEPWRAAYIEPCRRPTDGRYGDNPNRLQHYFQYQVVLKPAPADSQDLYLQSLAHLGLD
                     PYQHDIQFLQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGLELDPVTVELTYG
                     LERIAMYLQQVDNVYDLMWTEDVRYGDLYHESEVQFSVYNFEAADVDLIQTGFDRYEK
                     ECEQLLEHPRGLVLPAYDCCMKCSHLFNLLDARGAISVAQRTGYIGRVRNLARKCAKA
                     YLGQREQMGFPLLRQASASSRPHRKQQII"
     gene            9195..9515
                     /locus_tag="PKM72_07690"
     CDS             9195..9515
                     /locus_tag="PKM72_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60705.1"
                     /translation="MKSFSTLFLYASFGLATLTMGCAEMNGGATGASGAPASPTSPSS
                     SAETTAVTTGPTRVSTGTQGDTLEACISRIPADATAGQRMIATTSCERDAKARTSIDA
                     VPGK"
     gene            complement(9712..10158)
                     /locus_tag="PKM72_07695"
     CDS             complement(9712..10158)
                     /locus_tag="PKM72_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNP60706.1"
                     /translation="MKLGKRIDLQRWICWNVRSGLLWVIGFLVLQGSCEWGGPPTIMV
                     TAEEFRFSPTRIEWESDQPLRLLIRNQGRERHVFHSQELFGPDANVLWHQPRIALQET
                     NAVVLEPGHSIELFFTASAGLYPFRCWIKGHNGMEGTILIKDLSSS"
     gene            10277..11188
                     /gene="folB"
                     /locus_tag="PKM72_07700"
     CDS             10277..11188
                     /gene="folB"
                     /locus_tag="PKM72_07700"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNP60707.1"
                     /translation="MSSSIVVKGIQLSARCGVTETERQQPQPLLVDLIFRCPNQSAFQ
                     SDELSDTVDYGSITQRIQDIGEGQAFSLIETMAEKICQALFHEFPITRLKIWVRKIRP
                     PLKRIEGSVGVQLIRSRQPMASTDLMHLSPFLVDHLSRLPDGTALDVATGRGRHALYL
                     ASKGFSVHGIDRDADALHELQTQAQEAGLSSITTESIDLEVNPQHPPDLGTAMYDVIL
                     VFFYLNRPLFPQLIKALKSGGVIMYETFLLDNHIHRQHPRRKEFCLESNELLGLLQGL
                     SILHYDEGDHAGSSGPAFTARILARKP"
     gene            11200..12045
                     /locus_tag="PKM72_07705"
     CDS             11200..12045
                     /locus_tag="PKM72_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNP60708.1"
                     /translation="MSRIDLHTHTHFSDGSESPTALVELAHQQNVSILAVTDHDTTEG
                     LTEAMEAAQDLPVEIIPGIELSTEFEGRETHMLGYFIDLADPEFQTRLEQLRTTRFDR
                     LHHILDRLHNLHVEVSLAEVEHVAGSGTIGRPHIAQVLIEKGYVKGMKEAFDRFLGVR
                     GSAYVRRMVPEAAEIMTWITDAGGIPILAHPYWEGLGADKTESSCRTLMDQGLRGLEV
                     FYGTFSARQISINLNLAKKFDLLMTGGSDFHGTLKPDISVGTGRGSLRVPPKLIDHLR
                     QAAAR"
     gene            complement(12083..13105)
                     /locus_tag="PKM72_07710"
     CDS             complement(12083..13105)
                     /locus_tag="PKM72_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60709.1"
                     /translation="MNAMSSWYRPDPLTRDLIHLINARRHEHGDPSLAIEVLQALLDQ
                     DREHEVLTVLAALDESMAEWLFDLLAEAASSCLMEDESEDSEQYGILASFELSLQANL
                     EFPLKLKIDPVETADLLRRHLHVPPEAVITVEPRLLTDSTLEFLSLQKIHDHISNLAD
                     GQPRQAIAARLHPPVEATAFLFFEILGVPDTTLPEALSEDDCQAIMDHLVNQCPEVAK
                     APQLLEAPNYAAYALYDAQNAVRLHRLADETAAVWHDLEGPVRSRTVVHLHTQCGNGV
                     YMPVWTDLFDPDLPEDHGPIWTSPDVWVFTADLPIEWPGELLTNRLLAEGCTRFENHI
                     VEATDN"
     gene            complement(13208..13621)
                     /locus_tag="PKM72_07715"
     CDS             complement(13208..13621)
                     /locus_tag="PKM72_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNP60710.1"
                     /translation="MKSYREELWFQTKERREFRNITPQVERVVQQSGIQEGFVLVNAM
                     HITASVYINDDEPGLLHDYEQFLERLIPQTGTYRHNLTGEDNGDAHIKRQIMGREVVV
                     AITNGRLDFGPWEQIFYAEFDGRRRKRVLVKIIGE"
BASE COUNT         3284 a         3952 c         3264 g         3363 t
ORIGIN      
        1 ctcccccggc ggcccccagc aacgggattg cctgggcggc agctttttct gatacttgga
       61 tactgtaccg tgaggcaatt tcagacagaa gtctgaccaa tggcggagcg cctgaccccg
      121 ccaagccacg ttcaagcaga tacttgcccg cttcggtcat cgcacccgcc aacgccgccc
      181 tcactgcaaa atatcccgtt tccgccccgt catccgcttg actggtgcct cccatggcga
      241 acacctccaa acaggccagc ttgctcccgg cctccataat cggttctccc tcacttctcg
      301 cgatatccgc gatagatcgc aacatcagtg tcgtcgagat cggcaattct acgctcaacg
      361 ccatgagccc catcgatccc ccgattgcac cgctgacggt tgtcaaaacc cggtgagccc
      421 cattggccgg cttaccccga taccgttcat ttatactgga cacggccaga tttaacgctt
      481 tattcaaagc tttttgcgtg gtcgtcagaa gtttgttgga gaatccctcg ggtagcatgg
      541 tgatggcccg ttcaaccggc cctccgatga gattcgtgac tttggcggca aagctggggg
      601 tttctaagag atctactgct aatgctaaat actgctcgtc aactttattg agattggaca
      661 tgagcgcgat ctccccggtt tctctattgt gcacaggatt ggagtattct ctatggtaat
      721 gaatcgcaga caccgtcagc ctgcccgtta tcgatgacta agtaaccccc tcaccgtcgt
      781 ggctcaattg ttcccgtcat gagccaatcc ttcccgagct ttttgagttg gcaatccgcg
      841 agtggccagg ctcgatcaat ttttttcggc gacagtccgc cgatgaggcc aatggcatcc
      901 tgcccaccca gcagcattgg agccgtatat aatcttaccc gattcaccaa tccctggtga
      961 agcgcagtcg cattcacctt actgccgcct tcgatgagga ccgatgtaat gccctgcttc
     1021 cctaatcgag caagacaggc tttcaacgag actcgtcccg cttgccgagg caacacccaa
     1081 acctgaatgc cacgtttttc aaagtgttcg atttttttga ctggagcctg tgtggtcgta
     1141 cacagaatgg tgggctgctc ctccacccat tgcaacacct tggcattgga gggaatgcgc
     1201 aatcggctat cgagaatgac tctcaccggt tgccgtccca cccgtccgct ggtcatcgtt
     1261 tttcctctgg ccgataattc aggatcgtcg gcgatgaccg tgcccactcc caccagaatc
     1321 gcatccatcc ggttacgcag acgatggaca tcctgtcggg cgcgttcacc tgtgatccac
     1381 cgtgactcgc cggtggcggt cgccaatttt ccatccaacg tcatggcccc tttgagggtt
     1441 accaacgggc gtccggtcgt aatccaatat gcatagacct cattcaggtt tctggcatcc
     1501 tgttctaaca tgccaaccga tacggggata ttcgcgtgct tgagttgttg aagcccccgt
     1561 ccattgacct gtggattggg atcgatcatc gccacgcata tccgcgctag cgcggactga
     1621 ataaggagag ggacacaggg aggcgtgcgc ttgtgggtat ggcaacaggg ttctaacgtc
     1681 acatagagga cgccccctcg cgctcgaggg cccgcttgat ggagcgctaa tacttctgca
     1741 tgaggttcgc cggactgacg gtgataggct tctccgacga tctgcccatc cttgacgatc
     1801 acagccccta ccatgggatt gggactcgta cgccctttcc ctttggctgc gagggaaagg
     1861 gctcgcttca tgaaagtgtt atctgatggg acggacgtca ccgcctttta gcagccgcag
     1921 tcggtttttt acgtgtagga gagggagaag tctttttctt ggaggtgctc ttcttggctg
     1981 tggtctttgt cacaacattt ttcttcacgg tgctcttcgc cgccggtttc tttggggctg
     2041 atttttttgt ggccggtttc ttccccgcca cgactttcct cgtcgtgact tttttccctg
     2101 tcgttttctt tgttggcgac tttttaggcg ttttggcaga accggatttg gatgacaccg
     2161 cccgggattt gttgattatg cgaaacgttg atcgagactc acgttgactc tcctcacgtt
     2221 cgagcaccgc tactgccgct tgagccgcag cgaggcgtgc aattgccact cggtaaggcg
     2281 aacagctcac atagttcagg cctaattcat gacaaaaagc cacggaactc ggttcacccc
     2341 catgctctcc gcagattcca agtttaatta acgggcgggt cttccgccct tcctgaatcg
     2401 cccatttcat taaggctcca accccttcct gatccaatac cgcgaaggga tccgcctcca
     2461 aaatattttc ggcttttttg tcgatcgtgg ccttacacat ccggcatacc attttcttcc
     2521 aatcgacgtt agtccgttgg cagaccggac accggtcctg gcgatccaga taaaagccga
     2581 tatagggtcc tgcatcatcg cgcgaaaacc cgaacgtcgt ttgcgtcaga tcattggtcc
     2641 caaaggaaaa gaattcagca tgttcggcaa ttcgccctgc cgttacagcc gcacgaggca
     2701 gctcgatcat ggtaccgatc aagtaactca acttcatgcc atattccgcc atggtctttt
     2761 ccgctacgtc ttgaatcagc tccttttggg ctttcatctc tgacggcatt cccaccaacg
     2821 gaatcataat ttccggcaca atctttttgc cttcgcgcgc cacttcacag gccgcttcaa
     2881 aaatcccctg aacctgcatg cgcgtaattt ccggcatggt aattccaagc cgacatccac
     2941 gaagacccag catggggtta tattcatgta actcttcgac tcgatccagg atgcgctgtt
     3001 tctcctgaat gacttgaggg tctccattgg tcacttccaa tttcgcgatt tcaaccagga
     3061 gatgttctcg tttgggtaaa aactcatgga ggggaggatc gagcaagcga atcgtgacgg
     3121 gaaatccctt catttcccga tagagtccga taaaatcctg ccgttgaagg ggaagaagtt
     3181 gctctaaata cagctcccgt tcttctcttg aagatgccag gatcatctgc tgcatgatgg
     3241 gaacacggtc ttcggcaaag aacatgtgct ccgtccggca gaggccgatg ccctcggccc
     3301 cgaatcctcg cgcgatgtgt gcctgctcgg gaatgtccgc attggaccgg atcttgagca
     3361 ctctaaacgc atcggcccat tttaaaatcg tggaaaaata ctgatatttt tcggaatctt
     3421 tcgcatccat tcttccctga atgacctgga tcacttcgga gtccaccacc ggaatgtccc
     3481 ccgcataaac ctgtccggta aacccgttca aggagagaaa atctccttcg gacagaacca
     3541 tggacccaaa ctggaccttt ccatttggca agacctccac ggcatcacaa ccggcgatac
     3601 agacttttcc catctgacgg gccaccacgg ccgcatgcga tgtcattccg cccttcgccg
     3661 taagaaatcc ttcggctgca tgcatcccct gaatatcatc aggactggtt tcatgacgga
     3721 ccagaatggc acgttccccc ttggccttca tggccaccgc ttgatccgga gtgagggcaa
     3781 tatgcccggc cgccgctccc ggacccgccg gaagtccctt tccgacagac tgaaatttgg
     3841 cttcatcact gctttcgaat atggggtaca aatattgaga gagttgttcg ggtgcgaccc
     3901 gcttgaccgc ttctctacgg tcgatgagcc cctgacgaac catatccacg gcaatccgaa
     3961 ccgcagcaat acccgtccgt tttccaaccc gggtttgcaa catatacagt ttgccctctt
     4021 ggatcgtgaa ttccagatcg agcatgtccc ggtaatgttt ttccagagta cgctgggtct
     4081 tgatgagatc cttgtacgct cccggcaacg tgtcttgtaa cgccaacacc ggcattggag
     4141 ttcgaatccc ggccacaaca tcctcgcctt gggcattcaa cagacattcg ccaaaaaaga
     4201 ccgggacgcc gtttgctgga tttcgggtga aggccacccc cgttccactg gtgtccccca
     4261 tgttgccaaa taccatggac tggacattga cggccgttcc ccattcatcg ggaatatcgt
     4321 agagccggcg atacgtcacg gcacgatccc cgaaccagga atcaaacacc gcatccaccg
     4381 ccatccggag ttgttcaaag ggatcttgcg gaaagtctcg tgctgtctcg gttttaacca
     4441 attcctgata ttgaaccact aactccgcca aggcctgttc agacaaatcg gtatcatgct
     4501 tgaccccatg cgcctgtttc atggcttcca ggctatgttc aaaccgctca cggggaatgc
     4561 ccatcaccac gctgccaaac atggtgatga aacgccggta cgcatcaacg gcaaaccgtt
     4621 gattgccggt ctttttgcct aaaccctgaa ccgtttgttg gttgaggccg agattcaaga
     4681 ctgtatccat cataccgggc atggacgctc gtgccccgga ccgtaccgac accagcaacg
     4741 gattgtccgg atcgccgagt ctggccttca tggttttttc gacctgccgc aggccattca
     4801 gcgcttgatc ccacatcccg gctggaaagc gtttttttga ataaaaatat tccacacagg
     4861 cttcggtggt aatcgtgaac cctgaaggca cggaaatttt caggttggtc atttcggcca
     4921 atcccgaccc tttccctcct aagagttctt tcatgttgcc ggatccttca gcttttccgc
     4981 cgccaaagaa atagacatat tttttgtgcc gcatttcttc gctctccttt acccgaccca
     5041 ccggcctccc ggaaaaaaag acgtcagcac cggagaggac tgaatgcctg gcattcttct
     5101 cccgaccatt ctccctgttg tcaggagacc ctcacggtta acagtcttgt ttttcagaga
     5161 cgcctgggat aggcgtggtg tatgaatata aatatgtgag ctggttgtta gaacaaaggt
     5221 tggatacaca agcattcaaa ttgaatgatg atttacagat tgaagacggt cctgatattt
     5281 agctatcaat atttgctttg tccacatgcc ggatattatg acaactatgg caaaaatcat
     5341 gagcgcaatc aatcggtaat tctccgccgg gttccctggg acatacagat tgccatccgg
     5401 tcccatggcc agggtttttc cttccggcaa ccgatgcgtc tcacccgaga tatcggagaa
     5461 atcaagccat tcggagcaga ccttgaccaa cccgtccaca cccgcaacct ttaaccaggt
     5521 gacctgaaca caatgcccat tttccggatc aaagagttcc gattttgaca cgatgctctc
     5581 caaatcaaca ccgctcaccc aaagaccggt cagaattaag gcattgcaga caataataag
     5641 ggcaaccgtc acgaccaggg taaagcgttt caggcgtcgt gaaatctttg ggctcactcc
     5701 cccgtccgta ctacccccca tcggatgttc cttcccatga ccgtctcgct ttcacgaggg
     5761 cgtccaggaa tcctcttcca gcgacggtta tccacttgcc gattgcagac aggacaaatc
     5821 ggcaaccgtg agaaacaggt catccgtcgc ctttaataag gaaaggcgat tagcccgcgt
     5881 ctgtgggtct gaatcattca ccatcacagc tccgaagaac tcatcaatcg ggctcttgag
     5941 ttccaacaac gagagaaggg ctttcccgta attccggtca ttcaccgaat cgcggacttg
     6001 ctgttgcacc acttccaaag cctgaaacag ccgacgctcc gattcatgca caaaatgttc
     6061 aggcagggcc tgcgcgttcg tccattgttc tttttcgaca attcgatgag cccgcttaaa
     6121 tccgatcatc agcggatcaa aatccggctg aggcaccatg gcttccaagg cctgcatgcg
     6181 tgcgaccaaa tcagccatat cacagaccat cgaattggaa acatggatga ccgcttccat
     6241 gatatcttcc cgtaagccca gatgttgcct gccatagaac cgaagccggt caagaataaa
     6301 cgccaccaca tctgaaccgg tttgaggggt gccacgcacc ttcccacctt gtccatctaa
     6361 gatccgcagc gcctgatcca caacggaggt gatattcaac cgtaggggcg tttcggcgag
     6421 tatccgtacc aacccatacg cagcgcggcg caaccccaac ggatcttccg agccggacgg
     6481 agacatgccg gccgcaaaaa aagccacgat ggaatcacac cgatcagcca cggcgactag
     6541 gactcctggc agctctttcg gtaattggtc atctggaaac ctgggaaaat actgttcacc
     6601 aatggcgcga cacacctctg gagattcgcc atcatgcctg gcatattctt cccccatgac
     6661 cccttgcagg gtcggaaact cacctaccat tccggtcgtc aaatccgcct tggccagaaa
     6721 agcggcgcga cggcacatct cgtccagatc ctttccgccg atctgttcag ccatccatcc
     6781 gaccagatga cccacccgat ccactttttg acgaaccgtg cctaatttgt gatgcaacag
     6841 aaccccttcc aacgccggca ctcgttcatg gagtgggcgt ttcgagtctt ctgtaaagaa
     6901 atattgagcg tcactcaggc gagcggccag gactcgttca ttccctttgg tcatgagcgg
     6961 ggtatttccc cacggcatat tcgtcaccgc aataaatttt ggcaggagcc caccatcctt
     7021 ggtgatcagc gagaaaaatc cttgatgctc tttcatcgat gagatcagca ccggtttggg
     7081 aatcgctaaa aagtctttct gaaacgttcc gagaatagcg tgcggggatt ccacactgaa
     7141 gactgctgtt tcaatcagct catcccggta caatggatcc acctggcctt ttgccgcctt
     7201 agccaacgca gagacttgtt ccgtaatgag gctccgacgc tcttcaggat caaccagcac
     7261 ccccagtcgt ttcatggcct cgatataatt gttggcggtg gtaagaggaa acgcctgtcc
     7321 cggctttttc cctttcgctc gataaaatcg atgccctttc gtcatggcgc cggattgaat
     7381 tcccgcaaat tctattctga gcggttggtc gcctaataac gcgaccaccc agcgaatcgg
     7441 acgaccaaat tttgcccgcg aggcattcca gcgcatcgct ttcggaaagg ccagtttact
     7501 taagatctgc ggaagcgcct ccgtgatcac ccgcttggcc gactgacctc gttgatgcgt
     7561 ttcgaccgcc atgtaggccc cttttggcgt atctttccta cggagtcggt caaccggcat
     7621 tccttgagat ttggcaaacc ccaaggctgc cttcgtaggc tggcccgaag catctaaggc
     7681 tgcagagacg ggagggccta gtatttcctg agtcagggaa gcttgctgtg gttgcacacc
     7741 tttgaccagc aaggctaagc gccgcggcgt gcccacgacc ctcagggact catactccaa
     7801 ccggctgtcc gcaaagagcc gttgcccaag atttgccagg tcttccaatg cctgcgggat
     7861 aacagccgac ggcaattctt ccgtcccaat ttctaacaaa aacgggacag tctttggttg
     7921 agatttcggg acagacttga ccttgacgtt tttgcgggtc gttcttgatg gtgccataag
     7981 cctaattgcc acagccccgc ctcggggcat cgattcaaat gatctgttgt tttctatgag
     8041 gacgggagga agctgacgcc tgtcgtaaga gtgggaaccc catctgctcc cgttgaccaa
     8101 gataggcctt cgcacatttc ctggcaaggt ttcgaaccct gccgatatag cctgtccgtt
     8161 gagccacact gatcgctcct ctggcatcca acaaattaaa gagatgagag cacttcatac
     8221 agcaatcata agccggcagc accaatcccc gtggatgctc cagtaactgt tcacattcct
     8281 tttcgtagcg atcaaagccg gtctgaatca agtccacatc ggccgcctcg aagttataga
     8341 cagaaaattg aacttcggac tcatgataga gatctccgta tcgaacatct tcggtccaca
     8401 tcaggtcata cacgttgtcg acttgctgca gatacatagc aatgcgttcc aacccatacg
     8461 ttaactccac tgtgaccgga tccagctcca atcccccgat ctcctggaag taggtaaatt
     8521 gcgtgatctc catcccatcc agccggacct cccatcccaa cccccaggcc cctaacgtgg
     8581 gagactccca gtcatcctga agaaattgaa tatcgtgttg gtagggatct aaccctaaat
     8641 gggcaagact ttggagatac agatcctgcg aatccgcagg agccggtttt aacaccacct
     8701 gatattgaaa gtaatgctgg aggcgattcg gattatcccc gtatcggcca tcggttggtc
     8761 ttcgacaggg ttcaatatac gccgcacgcc aaggctcggg gccgatcgcg cgaagaaagg
     8821 ttgccggatt aaacgttcca gccccttttt cagtgtcata cggctgagtc aaaatgcagt
     8881 ggtgggattt ccaaaactga tgaagggaga gaataatgtc ttgaaaggtc acaacacttg
     8941 accctggccc ctaggttgac cccggaacag tcgagcaatg aggtacgcca tgattgattc
     9001 cgtccgtttt ctgcgcgtag ggaggaaaat tttacgttag caataaaccc taaatcagtc
     9061 aagattatcg gatgggaaac gactcaaatg cggaagagat cagtaaagaa gattttgctc
     9121 tccacacctt gttttttttt ggagtctcaa gtatatttcg cgacttgttt atctattgag
     9181 gaggggttcg accaatgaaa tcattttcta cactgttcct ctatgcttct ttcggtctcg
     9241 caaccttgac gatgggctgc gctgagatga atggcggcgc aaccggtgca agtggcgctc
     9301 cagcttcacc tacttcgccg tcctcaagcg ctgaaaccac agccgttacc acgggaccca
     9361 cccgagtctc cactggcacg cagggcgaca cgcttgaagc ctgtatctcc aggattcccg
     9421 ctgatgcaac tgctggccaa cgaatgatcg ccacgacgag ttgcgaacgt gacgccaagg
     9481 ccagaacctc cattgacgct gttccaggca aataggacca tgcttgctgg cggctcctgc
     9541 caccagcaag cgttttgtct ctcgccatca ttcagatcca aacaatccta gataattaaa
     9601 gtacctcagg tcttcgggct tctcaagcca cgatatacgc caacaagaag atccagggct
     9661 ttctggttcc ctgatggtgg tcctattgtg atttcatcaa gatgatctct attagcttga
     9721 ggagagatct ttgataagga tcgttccctc catgccgttg tgccctttta tccaacatcg
     9781 aaagggatag agaccggcag atgcagtaaa aaacaactca atcgaatgac ccggctcaag
     9841 cacaacggca ttggtctcct gcagcgcaat ccttggctga tgccatagga cgttagcatc
     9901 cggaccaaac aattcctgac tgtgaaatac atggcgttca cgtccctgat tccgaatcag
     9961 aagacgaaga ggctggtcgg attcccattc aatccgggta ggactaaatc gaaattcctc
    10021 agcagtcacc atgatggtgg gtggaccacc ccactcacac gacccctgca gaacgaggaa
    10081 accgataacc cacagcaggc ccgagcgaac gttccagcaa atccaacgct gtaggtcaat
    10141 ccttttcccc aatttcatca acatctcctg cattcaactc aggaaagccc tccattccgg
    10201 aacacgatgg ggactatcta tgggctcttt tcaaaaatca catgcacacg acgaggaagt
    10261 tctgacataa tggcccatgt cctcatccat cgtcgtcaaa ggtatccagt tgtccgcacg
    10321 atgcggggtc actgaaacgg aaagacagca acctcaaccc ctcctggttg atctcatttt
    10381 ccgatgcccc aatcaatcgg cctttcagag cgatgaatta tccgatacgg tcgattatgg
    10441 atcaatcacg caacgaattc aggacattgg ggaagggcaa gccttttccc tgattgaaac
    10501 aatggcggag aaaatctgcc aagccctgtt tcatgaattt cccattaccc gtcttaaaat
    10561 atgggtcaga aaaatccgcc ctccgttgaa gaggattgag gggtcagtcg gggttcagtt
    10621 gatccggtca cgccaaccaa tggcctcaac cgatctcatg catctttctc catttctggt
    10681 agaccacctc tcacgacttc ctgacggaac agcgttggat gtggcaacgg gacgaggtcg
    10741 tcatgcgctg tatctggctt ccaaagggtt ttccgttcat ggaattgatc gtgatgccga
    10801 cgcgctacat gagcttcaga cacaagccca ggaagccggt ctgtcctcga tcacaaccga
    10861 atccatagat ttagaagtga acccgcaaca tcctccggac ttgggcacgg ccatgtatga
    10921 tgtcattctc gtctttttct acctgaaccg gcctcttttt cctcaactca ttaaagctct
    10981 taaatccggt ggcgtcatca tgtatgaaac ctttcttctg gacaaccata tccatcgaca
    11041 acacccacgc cggaaagagt tctgtcttga atccaatgaa ttactcggac ttcttcaagg
    11101 gctcagcatt ctccattatg acgaaggaga tcatgcgggc tcttcaggac cggcgtttac
    11161 cgcacgaatc ttagcacgca aaccataatt cccaatcata tgagccgtat cgaccttcat
    11221 actcacaccc atttctcgga cggaagcgaa tctcccaccg cactcgtgga gcttgctcat
    11281 caacaaaacg tgagcatcct ggcggtaacc gaccatgaca ccactgaggg cctcacggaa
    11341 gccatggagg ccgctcagga cctcccggtt gaaatcatac ccggcattga attaagtaca
    11401 gagtttgaag ggcgggaaac gcatatgctc gggtatttca ttgatctcgc tgatcctgag
    11461 tttcaaacca ggcttgagca gcttcgcacg acacgctttg atcggcttca ccacatcctt
    11521 gaccggctcc acaacctcca tgtagaggtt tctctcgctg aagtggagca tgtcgccgga
    11581 agcggaacga tcggtcgccc acacattgca caggtattaa ttgaaaaagg gtatgtgaag
    11641 ggaatgaaag aagcgttcga tcgcttcctg ggggtcagag gctcagctta tgttcgacga
    11701 atggtccctg aagcagccga aatcatgacg tggatcacag acgctggggg catccccata
    11761 cttgctcacc cctattggga gggattgggt gcggataaga cggagtcctc atgccggacg
    11821 ttgatggacc agggattacg ggggcttgaa gtcttctatg gaaccttttc cgctcgtcaa
    11881 atttcaatca atctcaactt agccaaaaaa tttgacctgc tcatgacagg aggcagtgat
    11941 tttcacggca ccttgaagcc tgatatttcg gtcggaaccg ggcgtggttc gctacgggtc
    12001 cctcccaagt tgatcgatca cttacgtcag gcggctgccc ggtaataccc aatacgcatt
    12061 gacgagaccc cataacgatt gttcaattgt ccgtggcttc cacaatgtgg ttttcaaatc
    12121 gcgtgcagcc ttcagccagc aggcgattgg ttaacaattc cccaggccat tcaatgggta
    12181 agtctgccgt aaacacccac acatccggag acgtccaaat cggtccatgg tcttcaggta
    12241 gatccggatc aaacagatcc gtccacacgg gcatatacac gccattcccg cattgagtat
    12301 gcagatgaac gacggttcga cttcgaaccg ggccttccag gtcatgccac accgcagctg
    12361 tttcatcagc caatcgatga agacgaacgg cgttttgtgc atcatacaag gcataggcgg
    12421 cgtaattagg cgcttccaat aattgaggcg ccttggccac ttcgggacat tgattgacga
    12481 gatgatccat aattgcctga caatcatcct cggaaagagc ttccggtaac gtggtgtcag
    12541 gaacgccaag aatttcaaaa aataaaaatg cggtggcttc gaccggtgga tgaagccttg
    12601 ccgcaatcgc ctggcgcggc tgtccatcag ccagattgga aatatggtcg tgaatcttct
    12661 gcaagcttaa aaattccagg gtagaatcgg taagaagcct tggttcaacc gtaatcacgg
    12721 cctctggcgg gacatgcaga tgtctccgta acaaatccgc ggtttccaca ggatcgattt
    12781 tcaacttcaa cggaaactcc agattggcct ggagtgataa ttcaaatgag gccagaatcc
    12841 catactgctc gctgtcttcg ctctcatcct ccatcagaca cgagctggcc gcttccgcca
    12901 acaaatcgaa taaccactcc gccatacttt cgtctaaggc tgcaagaacg gtaagcacct
    12961 catgttcgcg gtcctgatcg agcaacgcct gcaacacttc aatggctaaa ctgggatccc
    13021 catgttcgtg tcgccgggca ttaatgagat gaattaaatc ccgggtgagg ggatcaggac
    13081 gataccaact agacattgca ttcatgggtg taaacgatcc aggcggaggt aaaaaatact
    13141 tctgaaaagg caaatcatca aaatatccag ggtattttca catgaccgga acccgtttgc
    13201 caactcatta ctctccaatg atcttcacca acacccgctt ccgacgtctg ccatcaaatt
    13261 ccgcgtaaaa gatttgttcc caaggaccaa agtccaagcg tccattggtg atagccacga
    13321 caacttcccg gcccatgatt tggcgtttga tatgtgcgtc accattgtcc tctccggtga
    13381 ggttgtggcg atacgttccc gtctgcggaa tcagacgctc aagaaactgt tcataatcat
    13441 gtagcaatcc gggttcatcg tcattaatgt aaacgctcgc ggtgatatgc atggcattca
    13501 ccaggacaaa tccctcttga atcccactct gttgtaccac acgttccacc tgaggggtaa
    13561 tattccgaaa ttcccttcgt tctttggtct gaaaccagag ttcttctcga tacgatttca
    13621 tgatgtcccc caaatcaaag attccatacc aggtctattt tccataacgc cgatcacgat
    13681 atcaatcgtc attattccca ggcacaactg actgcccgga tcctttcatc cttcgaatga
    13741 actcgatcgt tttcccaagg cgttctgttc gcgggaagac gcgaccatgt ggcctcattg
    13801 atatggctgg aagatttgac aggaaataga agtgatgtta ataaacttca tggatgggta
    13861 ttt
//
LOCUS       DAORAQ010000065        13651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_749_length_13651_cov_59.404972, whole genome shotgun sequence.
ACCESSION   DAORAQ010000065 DAORAQ010000000
VERSION     DAORAQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13651
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_13651_cov_59.404972"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..1136)
                     /locus_tag="PKM72_07720"
     CDS             complement(282..1136)
                     /locus_tag="PKM72_07720"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNP60711.1"
                     /translation="MARDRILKEIQTLDPQKDHERIVFLSTCYDFSFDTTRALELALF
                     RTFCVPSISGLLDRTGEFGQRTQKRYDDTDILISELLEWGYSSERGRRAIQRINKLHG
                     RFSINNEDFLYVLSTFLFEPIRWNCRFGWRIMCEQERLGLFYFWRAVGQRMHIQEIPE
                     DYQTFERFNVEYERHHFQYAPSNYRTGMATVNMFSGWFPKVLAPSVQSAIYSLLEPPL
                     LKAFGFPQPSTFMRWAVPSLLKLRASALRWLPPRRHPRLRTEMSHPSHPHGYVIEQLG
                     PPEETKGK"
     gene            1613..2686
                     /locus_tag="PKM72_07725"
     CDS             1613..2686
                     /locus_tag="PKM72_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNP60712.1"
                     /translation="MSAHRNIMVLLAALLSVMALSGAVARAEVDFYSHDELNLRPVIG
                     VFSQETETITGSSTIDPAVKAELSRYRYMIPASYVKWVGQAGGRVLPILLNQPEGYYD
                     EIFEQTNGILFPGGNQGIDPSDIYTEEGEILWNLAKEANDRGDYYPIWGTCLGFEELS
                     VLETGNGNVINLNVVATNLALSLRFTRDARQSRLFTSFPSKLLRAIRTQRLTFNSHDH
                     GLLVSEYRSNPSLYLFFDMLSFNEAPAGQVFVSTMEARDYPFYGTQWHPEKNNFEWSQ
                     NSDYSNIPHSPNAILVSEATARFFLSEARKSGHIFPEARRDELIYSAPIIYTGKGDWI
                     YEQVYVFCPTDARSCSLSKELAE"
     gene            complement(2965..4569)
                     /locus_tag="PKM72_07730"
     CDS             complement(2965..4569)
                     /locus_tag="PKM72_07730"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNP60713.1"
                     /translation="MKIGQVPYRLLVTTVGLICILFIPSNAWAFELEEATITDLQEGM
                     RMGTYTSRSIVEMYLKRVEEIDKRGPAIRSYLEINPDALAIADQLDQERRDEQIRGPL
                     HGIPVLLKDSIDTADKLSTTGGSYALEGIPVPRDSFVAKQLRKAGAVIIGKANMSEWG
                     YMRSTRASSGWSARGGQTKNPYILTRNPCGSSSGSGAAVAANLVAVAIGAEVDGSIVC
                     PASANSIVGIKPTVGLVSRDGMIPAAPTQDTAGPLTRTVADAAIVLGALTGIDPRDPR
                     TEESRGKSHADYTQFLDPKGLHGARIGIARNYFGYHEKVDALINRSIEVMKQQGALII
                     DPANVETAGQFGNSELTVLLYEFKTALEKYLRTRSSTGPQTIKDLMAFNENHRSLEMP
                     YFEQEIFEMADKTGPLTEKAYLDALERNRRLAATEGIDATIKKYELDAIVAPTYGPVM
                     PIDLILGDHLVPPWKSQEWTPSAPAVAGYPHISVPAGYIFGIPVGISFIGGAYSEPTL
                     IKIAYAFEEATKYRKPPQFLAQDLAR"
     gene            4886..5983
                     /locus_tag="PKM72_07735"
     CDS             4886..5983
                     /locus_tag="PKM72_07735"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNP60714.1"
                     /translation="MMYPHTISRFILVVLVMSVCMVEEVQSSSLCQSPGFPRWTFDQS
                     HIFPQDRPLARPEDGKALPDGRLVVADERFGLYLIEPNNSHRPFGRFSEVGYVHTPPD
                     PAGGVQAVFLEHDTRHLLVGDIYSGKIYRVNTQTEETRLIYDHPYGVNSVYRDRNGTI
                     WFTQSTNNIEARGKEDLWASVNLPVPTGAVFKLPGSGEGFAEKAEEVANNLYFANGIT
                     FDKTEKYMYVSESMMDRVLRFRVDVESGSVSESEVYQNVYVPDNLAVDSDNNLWIASF
                     VGNKVMVVDNICRAVHTVFQATSTSHSDFLKEWTKRSHLGQPRNEVLTPEAFNPLPNF
                     LTGLFFSPNFDTVYFTGLGNAILAYAMSTGR"
     gene            complement(6011..7273)
                     /locus_tag="PKM72_07740"
     CDS             complement(6011..7273)
                     /locus_tag="PKM72_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP60715.1"
                     /translation="MESEAPSSSRQRIILAGLIGNVMEWYDFAVYGYFAAIIGRQFFP
                     SDNPAVSLITAFGAFAAGFLVRPLGGLLFGRIGDLIGRTRALTLSVITMAVPTVFIGL
                     LPTHAEIGLAAPVLLVAFRMIQGLSVGGEYTSSLVFLVENSPHNRRAFSAIWGVWGAG
                     AGILLGSGVGALLTNGLDEAQVVSWGWRLPFILGALVAGTGYFVRRNIHTERPVGETT
                     SPVLDSFGKHRSSVMKVALLNVGYGISFYAAFVYAVTYIKEIDHLPEGVAFNLNTLSM
                     ALLLLFLPMGAWLSDKIGRKPVLIAAGTLMTFGAIPLFLLIHTTHPITILLGELGFAL
                     IIGMLAGGIVAANVELIPAPVRCTGLAFAYNLSIGVFGGTTPLMAAWLISATGNPIMP
                     AYWIAMGGAVTLLTSIFLIRETAFIELD"
     gene            complement(7408..8250)
                     /locus_tag="PKM72_07745"
     CDS             complement(7408..8250)
                     /locus_tag="PKM72_07745"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNP60716.1"
                     /translation="MVGAVLSSKPMYFPPAPFDLAEAVECSDLVDMAYGMYYQWVQQG
                     KPHTPNDLRWQLPHNSPYRFGEPIWGNSEVLVFWDLEPFALVAQRDDKAYAIFRGTET
                     LEDWYKDAEVKQVPYELPHISNVGLVHEGFFDLYKSLRLPLLSQLNALQGIRTLYITG
                     HSLGAALAMVSTPGLMANALTQHTPNVFQYNLAGPRACDPKLATFLNTGLAYIYRIVN
                     SCDLVPEVPPSVYLGFTEKVLYEHVGYPVTFTAQYGDVPGNHAHHTSYNYALKNPQQP
                     QGTV"
     gene            8384..8545
                     /locus_tag="PKM72_07750"
                     /pseudo
     CDS             8384..8545
                     /locus_tag="PKM72_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8689..9582)
                     /locus_tag="PKM72_07755"
     CDS             complement(8689..9582)
                     /locus_tag="PKM72_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP60717.1"
                     /translation="MPNHRLRQPLIRSVVHCSDFSPGSHTAFAHALKAALTAKAKLSI
                     LHVADDMEANWTEFPGVRNILERWELLPRNSPRSAVPELGINVRKLTIQHKDPVKSVV
                     EWLEHHPTDLLVLATQQDNNRVFWMSRSVAKPITRKSRLMTLLIPKGIMGFVSKQDGA
                     VSLKHIVIPVAADPKPQPAIQAAARLVQQLNCPSGRFTVLHVGENGQMPTVRFSSVPG
                     WSWTKVTEDGDISKIILKTVKDHKADLLIMTTAGRRGFLDALRGSQTEQVLRKASCPV
                     LTIPAGGWMASLLETETDGDT"
     gene            9844..10710
                     /locus_tag="PKM72_07760"
     CDS             9844..10710
                     /locus_tag="PKM72_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HNP60718.1"
                     /translation="MSKWVAIVLFIVAAFTQTIGGLASGTATRTLCSDSWYRLIEEKV
                     PTSDEQGHGPDVGSDEWKSVIEFKLGIRDKPDVPSRDTEAWCRHIDQMVLENRTSSKS
                     VGDPGGAAKAQGPSFACINVIGGSIEAMICKDEELSALDRKLSDVYASASKKASNEHP
                     SVLKTEQRGWIKGRDDCWKSEDTRGCVRDEYTRRIAELQARYRLVPHSGPVSFLCGDT
                     SANEVVAMFFQTQPPTLIAERGDSVSLMFLQPSGSGTKYQGRNETFWEHQGEALITWG
                     YGAPSMHCKKTS"
     gene            complement(10732..10857)
                     /locus_tag="PKM72_07765"
     CDS             complement(10732..10857)
                     /locus_tag="PKM72_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60719.1"
                     /translation="MPGNRIGVDAAVAQVAGAGIGGSGYTDQKQRCGQFVIFGDG"
     gene            11076..12101
                     /locus_tag="PKM72_07770"
     CDS             11076..12101
                     /locus_tag="PKM72_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNP60720.1"
                     /translation="MNIAIDESGTFVYTDTDNSWNCVVSYVYPEVHNPHIQEEVRKLV
                     QRHAKGGQKEVKLRNLSESAYANFLRCLQKWDGVLYAVATNAAANTPQVLECHQEEQT
                     QKILEHLDKMIYETGREGVRRLAKRVKALPHQLYVQMICQVQLVIQILHSALLFYVQR
                     HPPTLNRFRWRIDQKNSSQTSYEDAYSQVLPAILQSASICKPIPMLKGEDYHWFDRFY
                     FPKGEVPTYLRDVYGVEIVDDNERKINIGQVVHEDVRFVDSKSDSGVQIADLLASGLR
                     RCLRGQFTDNNIIASLIGGLMVQREHNQIPIQLISLGKEEKKVEGQVLSALHAMAIQS
                     RAMIVRQ"
     gene            12287..12604
                     /locus_tag="PKM72_07775"
                     /pseudo
     CDS             12287..12604
                     /locus_tag="PKM72_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            12608..12850
                     /locus_tag="PKM72_07780"
     CDS             12608..12850
                     /locus_tag="PKM72_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60721.1"
                     /translation="MVAGLKPHGVYLVEAYTPDQLKHGTGGGKSADSMTTKESLSLEL
                     EGLKFRHLLELERDVVEGIYHTGLGAVVQAVASKEI"
     gene            13192..13644
                     /locus_tag="PKM72_07785"
     CDS             13192..13644
                     /locus_tag="PKM72_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNP60722.1"
                     /translation="MIKHFDHITIVVRDVEEAKQFFGLLGFKEDKSVVIAGPQFSNYM
                     GVDGIEAEHITLVLANVSPRTEVQLLKYRSPDPLLDPAIRNLTKLGFNHVCFAVDDLE
                     AEVAKLQAHGVHLRNEVMDFHDRKLVFLSGPEGITVELAEWDQSRSKR"
BASE COUNT         3457 a         3388 c         3538 g         3268 t
ORIGIN      
        1 ccctctggac gatggggtat aaaaatattg tgtatctgga cgggcacatg tcaggttgga
       61 aacagcgagg catgctcatg gaaaaccgcg ccgataaatc gaataattaa gtactaagcc
      121 gccgcctcca ccttatactt aaccctctcg caggactcta tgttcatgat tgactctcca
      181 atttgtggat aaataggtct atgagaagaa ctgctatttt ttcgcgtttg cgtggtcctg
      241 ttttacgtgg ataagagata gccagcctac cttccctacc ctcattttcc tttcgtttct
      301 tctgggggac ccagttgttc gatcacatag ccgtgaggat gtgagggatg gctcatctcg
      361 gtccggagcc ggggatgccg tcgaggggga agccaccgca acgcactggc tcgaagcttc
      421 agaagtgagg gtaccgccca tcgcataaat gtagacggtt gaggaaagcc aaacgcctta
      481 agtaggggcg gctccaaaag ggaatagata gcggactgga cagaaggtgc caacactttt
      541 gggaaccatc ctgaaaacat attcacggtc gccatgcctg tacggtaatt tgatggcgcg
      601 tactgaaagt ggtggcgttc atactctaca ttgaaacgct caaaggtctg gtaatcctct
      661 ggaatttcct ggatgtgcat gcgttgtccg acggctcgcc agaagtaaaa taggccgaga
      721 cgttcctgct cgcacatgat tcgccagccg aaccgacagt tccaccgaat gggttcgaag
      781 agaaacgtgg acagaacata gagaaagtct tcgttgttta tcgagaatcg tccgtgtagc
      841 ttgtttatgc gttggatggc ccgccgtccc cgttcactgc tgtatcccca ttccaataat
      901 tcgctgatca gaatgtcggt gtcgtcatag cgtttttgag ttcgttgccc gaattctccg
      961 gtccgatcca gcagcccgga aatactcggc acacaaaagg tgcgaaacaa ggccagctcc
     1021 agagcgcggg tcgtgtcgaa ggaaaaatcg taacaggtgg acaaaaagac aatacgttca
     1081 tgatcctttt gtggatcgag cgtttggatt tccttgagta tgcggtctcg cgccatcgtc
     1141 acaatctctc acttttctta catccggtca tatcccacca taaacttctt aacggatcaa
     1201 taaaagaagt gtgacttgga aagggttttc aggactctaa ttaagaatcg tttttggatt
     1261 atgccatatt agggagggct tcccctcggc gaaagcttgg ggaactgctg gcgagggctt
     1321 ttcttgtggt ccgaaatttt ctcattttgg gttagaatag ctgcggggct gttcctcttt
     1381 tcaagctgtg gaagactagg gcaatctcta aagcaaattg gcacagcagg agatccgtca
     1441 tgcattccgg tcatcgtcat taccggaatc tttatggtcg atttgtgatg gcagatcgaa
     1501 atacgatcct gtcccgggtt cgttgaatca taccgtttga gtgccgggaa aactgcacag
     1561 tccattgtat atattaaggt ggcctaaaaa tttttaacga ggagatcatc atatgtcagc
     1621 acatcgcaat atcatggttc ttctggctgc gcttctgtct gtcatggcct tgtccggcgc
     1681 tgttgcccgt gcagaagtcg acttctattc ccacgatgaa ctgaatctcc gtcccgtaat
     1741 cggtgttttc agtcaagaga ccgagacgat caccggatcg agcacaatcg accccgcagt
     1801 gaaagcggag ctctcgcgat atcggtatat gattcccgcg agttacgtga aatgggttgg
     1861 acaggcaggt ggacgggtgc tgcctattct cctgaatcag ccggaggggt actacgatga
     1921 aatcttcgaa cagacgaacg ggattctttt tcctggcgga aatcagggga tcgatccgtc
     1981 ggacatttat acggaggagg gggagatcct ctggaactta gccaaggagg cgaatgatcg
     2041 tggcgactac tacccgatat gggggacatg tctggggttc gaagagcttt cggtgttgga
     2101 gacgggcaac ggcaatgtga tcaacctcaa tgtggtggcg acgaatctgg ccctgtctct
     2161 gcggttcact cgtgatgcga gacagagcag gctctttacg tcgtttccct caaagctcct
     2221 aagggcgatt cgaacccaga ggctgacctt caactcccac gaccacgggc tactggtcag
     2281 cgaataccgg tcgaacccat cgctgtattt gttctttgac atgctgtcgt tcaatgaagc
     2341 gcctgcgggt caggtgtttg tgagcaccat ggaggcacga gattatccat tctacggcac
     2401 gcagtggcac ccggagaaga acaatttcga atggagccag aactctgact attcaaacat
     2461 cccccattcg ccgaacgcca tactggtttc cgaagccacg gcccggtttt tccttagtga
     2521 agcgagaaag tccgggcaca tctttcccga agcccgacgc gacgaactga tctactccgc
     2581 cccgatcatc tacacgggta agggcgactg gatctacgag caggtctacg tcttttgtcc
     2641 aacggacgcg aggtcttgtt cactgagtaa agaattagca gagtgatcgc gggcaccgtg
     2701 tgagtggatt gtggcaactg aaggacatgt tgtagatacc atcacgattt ttcagtaagt
     2761 aatttggtgc tgtctcaaag gggaagcctt cgatgatttg tggtgctggt ccatagcacc
     2821 gttggttgtt cggttacatt tgacgcaaca tcagcttctc acaatatttt tgccctgcgt
     2881 gtcacgcgat cagaggggag cacaaaatca gattttattg tgtcccgact gatccttttt
     2941 agaacctccc atcattttag acagtcaccg ggccaaatct tgtgcaagaa actggggtgg
     3001 tttgcgatat ttggtggcct cttcaaacgc ataggctatt ttaatcaggg ttggttcgct
     3061 gtaggccccc ccgataaagg aaatgccgac cggaatcccg aagatatatc cggcgggaac
     3121 cgagatatgc ggataaccgg ccacagccgg agcggaggga gtccactctt ggctcttcca
     3181 aggtggaacg agatgatcgc ctagaatgag gtcaatcggc ataaccggcc cgtaggtggg
     3241 agccacgatg gcgtccagct catatttctt tatggtggca tcaatccctt ccgtggcggc
     3301 aagccgacga tttcgctcca aggcatccaa ataggctttt tctgttaacg gacctgtctt
     3361 gtcagccatc tcaaaaattt cctgttcaaa gtacggcatc tcgaggcttc ggtggttctc
     3421 attgaaggcc ataagatcct tgatcgtttg aggcccggta gaggaacgtg tgcggagata
     3481 tttttcaaga gcggtcttaa attcatagag caacacggtc aattcggagt taccgaattg
     3541 tcctgccgtt tccacatttg cgggatcgat aataagagcc ccctgttgtt tcatgacctc
     3601 gatggaccta ttgatgagag cgtcaacctt ttcgtgatac ccaaaataat ttctggcaat
     3661 cccgatacgt gctccatgta atcccttggg gtccagaaat tgggtatagt ccgcatggga
     3721 ttttccacga gactcttcag tacgggggtc acggggatct atccctgtca acgcgccgag
     3781 cacaatggct gcatcagcca ccgttcgagt caggggacct gcggtatcct gggtcggtgc
     3841 agcaggaatc atgccgtcgc ggctcacaag gcccaccgta ggcttgatac ccacgatgga
     3901 attggctgat gccggacaga caatcgaccc atccacttcg gccccgatgg ccacagctac
     3961 taagtttgcg gccacggctg ccccggatcc cgaactggac ccgcaaggat tccgtgtcaa
     4021 gatataggga ttcttggttt gacctccccg ggcgctccat ccgctgctgg cgcgagtgga
     4081 ccgcatgtaa ccccactccg acatattcgc tttgccgatt atcacggctc cggctttccg
     4141 gagttgtttg gccacaaaag aatccctggg cacaggaatg ccttccaacg cataggaacc
     4201 gccggtggta ctcagtttgt ccgccgtatc aatactatcc ttcaacagaa ccggaattcc
     4261 gtgtaatgga ccacgaatct gttcgtcacg acgttcttga tccagttgat ccgcgatggc
     4321 caacgcatca ggattgatct ccagatatga tcgaatcgcc ggaccccttt tgtcgatttc
     4381 ttctacccgt ttcaaataca tttcgacaat tgatcgcgac gtgtacgttc ccatccgcat
     4441 cccttcttgc aggtcggtga tggtcgcctc ttccagttcg aaggcccatg cattcgacgg
     4501 aatgaaaagg atgcaaatca agcctacggt ggtgactaag agacggtacg gcacttgacc
     4561 aattttcatc cgggttacct ctctcatggc gtttgtttgt gacccctcat tttcaaattg
     4621 ttaggcagcc ggtccgacga taactatata gaacaaaaaa gccaagccat ccgtgggatc
     4681 catacctact cgaaaccctc tccataatgt cgaatctccc gaaatttttc cggtagaata
     4741 gttacaacgg caaggtgaaa ttttgactct tctccctttt tccctcttac tcttctcttg
     4801 attttggtcg atcaatcggc gtgggtttat tcaggtgatc acagactcac tccaaatagc
     4861 ctgggacagc tttaagaaag gacgaatgat gtatcctcac acgatctctc gtttcatatt
     4921 ggttgtgtta gtcatgagtg tttgtatggt tgaggaagtt caatcatcct ctctatgcca
     4981 atctcctggt tttccacgat ggacattcga tcaatcgcat atttttcctc aagatagacc
     5041 gttagcccga cctgaagacg ggaaagcctt acctgatggt cgcctggtcg tggcggatga
     5101 aagattcggt ctctacctta ttgagccaaa caattcccat cgcccttttg gtcggttcag
     5161 cgaggtggga tacgttcata ctcccccgga ccctgccgga ggcgttcaag ctgtgtttct
     5221 ggagcatgac acccgtcatc tgcttgtagg ggatatttat tcaggaaaaa tttacagagt
     5281 aaatactcaa accgaagaga cgcggcttat ttatgatcac ccgtatgggg tcaacagtgt
     5341 gtatcgagac cgcaacggga cgatctggtt tactcagtcg accaacaata tagaagcccg
     5401 tgggaaagaa gatctctggg ccagcgtaaa cttacctgtc ccgacgggag ctgtttttaa
     5461 attgccgggg tcgggggagg ggtttgctga gaaagccgaa gaagttgcca acaatcttta
     5521 cttcgccaac ggaatcacgt tcgacaagac agagaagtat atgtatgtgt cggaatcgat
     5581 gatggatcgc gtgcttcgtt tccgtgttga tgttgagagc ggctcggtct cggagagcga
     5641 ggtctaccag aacgtgtatg taccggacaa tctagccgtc gacagtgaca acaatctatg
     5701 gatcgcttca tttgtaggga ataaagtcat ggtagtcgat aatatttgtc gcgcggtgca
     5761 cacggtcttt caggcgacgt cgacaagcca ctctgacttt ttgaaggaat ggaccaagcg
     5821 tagtcatctt ggtcagcctc gtaatgaagt gttgacaccg gaggctttca acccattgcc
     5881 gaattttctc acggggctgt tcttttctcc taatttcgac acagtctact ttacaggcct
     5941 gggcaacgcc atccttgcat atgccatgtc gacaggccga taacggagaa agtcgtgggc
     6001 cacgatcaaa tcagtcaagt tcgatgaacg ccgtttcgcg aataaggaag attgatgtca
     6061 gcaaggtgac cgctccaccc atggcgatcc aatacgccgg catgatcgga ttgcctgtcg
     6121 ctgaaatcag ccaggcggcc atcagcggcg tagtgccgcc aaacacgccg atcgaaaggt
     6181 tatacgcaaa cgcgaggccg gtgcatcgga cgggagccgg aatcaattcc acattggcgg
     6241 caacaatccc tccggctaac attccgatga tcaacgcgaa ccccaattcc ccaaggagaa
     6301 tagttatcgg atgagtcgtg tggatcaaaa ggaaaagcgg aatggctccg aaggtcatca
     6361 gcgtaccggc agcgatcaat acaggcttac gcccgatttt atccgatagc caggcgccca
     6421 tcggaaggaa caataacaac agggccatgc ttaacgtgtt cagattaaag gccacgccct
     6481 caggcaaatg atcgatttcc ttgatgtagg tgacggcata gacaaaagcc gcgtaaaaag
     6541 aaatgccata cccaacatta aggagtgcga ccttcatgac tgatgagcgg tgtttcccaa
     6601 aggaatcgag gaccgggctt gtcgtttcac cgacaggcct ttcagtgtgg atattacgcc
     6661 ggacaaaata tccagtgccg gctaccagcg cacccaggat aaatggaaga cgccaacccc
     6721 aactgactac ttgtgcctca tcaagcccat tcgtcaggag tgccccgaca ccggagccta
     6781 agaggatccc ggctcctgcc ccccagacac cccaaatggc actgaaggct cttcgattgt
     6841 gcggagaatt ttctaccaaa aaaaccaagg aactcgtgta ctcaccgccc accgataatc
     6901 cctgaatcat tcgaaaagcg accagcaaaa caggggccgc caatccgatt tccgcgtgag
     6961 taggtaaaag accaataaac actgtgggaa cagccatggt aatgactgaa agcgtcagcg
     7021 cgcgggtgcg tccgattaag tcaccgatcc ggccaaacaa caaacctccc aagggccgaa
     7081 ccaaaaagcc tgcggcgaac gcaccgaagg ccgtgatcaa tgaaacggct ggattatcgg
     7141 acggaaaaaa ttggcgtcca atgatggcgg caaaatagcc gtacacggca aagtcatacc
     7201 actccatcac gttgccgatg agcccggcta ggataatgcg ttgccgggac gaagatggcg
     7261 cttcactctc caatgacttg aaatcccttc actcccacgc gaacctcgca tgagctattc
     7321 attcgctcta aaccgtttta tatgtgcgat catgtcgact ttctggtttt ggtgttgatg
     7381 caagggccct acccttccat gactggttta tacagtgcct tgaggttgtt ggggattttt
     7441 taacgcgtaa ttgtaactgg tgtgatgagc atggttaccg gggacatccc cgtattgcgc
     7501 ggtgaaggtc acagggtagc ccacatgttc atacaggacc ttttccgtaa aaccgaggta
     7561 caccgacggg ggcacttccg gcaccagatc gcaactattc acaatccgat aaatgtaagc
     7621 gagaccggta ttaagaaagg tggccaactt tggatcgcaa gcacggggac ccgctaaatt
     7681 gtattggaag acgtttggag tatgttgcgt caacgcgttg gccattaagc ccggtgtaga
     7741 taccatggcc aaggctgcac ccagactgtg gccggtgatg tacaacgtgc gaatgccttg
     7801 cagcgcattg agttgcgata agagggggag acgaagggat ttatacaggt caaagaatcc
     7861 ttcatgcacg agaccaacat tggaaatatg tggcagctca taaggaactt gtttgacttc
     7921 cgcatccttg taccaatcct ccagggtttc cgtcccgcgg aaaatggcat aagccttgtc
     7981 gtcccgctgg gcgaccaagg caaagggttc caaatcccaa aacacaagta cctcggaatt
     8041 gccccagatc ggttcgccga agcgatacgg cgagttatga ggaagttgcc aacgtaaatc
     8101 atttggcgtg tggggtttac cctgctgtac ccattggtag tacataccat aggccatatc
     8161 caccagatcg gaacattcca ccgcctcggc taaatcgaat ggggccggtg ggaaatacat
     8221 gggtttgctc gacaggactg ctcctaccat ccatcaccaa tcagactggt ggcaccggac
     8281 ggtaaagaag aaaaagggcg agacctaggg ggattggctc ggggcgggtc gaaaacctgt
     8341 aaccgagatg acttcccctg ggtattgacc ccggactcaa gccaatgtga ttgatccaga
     8401 tgccaccatc atctttacct atgggctgtc ggcgggtggt tcgttgaaga tcgccatgga
     8461 ggtccaccat ccggaaaagc cctatcatca cgtggatctg aagaagacga ctcgcaagca
     8521 aacggttgac gaaattgtgc ggtagctgac aggagatgag gaattgagtg accatgatgc
     8581 gaataaaccg acaccaccaa aaagtggggt atgctgacaa taccaacagg gctgttaggc
     8641 gcagctcgtt acgcggtatt taaagaatca actgaaaata agtttgggct aagtgtctcc
     8701 atccgtttcg gtctccagta aagaggccat ccatcctcca gccggaatcg tcaagaccgg
     8761 gcaggatgct tttctaagaa cttgttccgt ctgactgccc cgcaacgcat ccaagaatcc
     8821 gcgacgccct gccgttgtca tgattaagag atctgcttta tggtccttga cggtcttcag
     8881 aataatcttt gaaatgtcgc cgtcttctgt gaccttggtc caactccacc ctggtactga
     8941 cgaaaatcgt acggtcggca tttggccgtt ctcgccgacg tggaggacgg tgaaccgacc
     9001 ggacgggcaa ttgagttgtt gcacaagacg ggcggctgcc tggatcgcag gttggggttt
     9061 aggatcagct gccaccggga ttacaatatg cttcaatgag acggcaccat cttgcttaga
     9121 cacaaaaccc atgattccct tcggtatcaa gagggtcatc aagcgtgatt tcctcgtaat
     9181 cggcttggcc accgacctgc tcatccagaa aacccggttg ttgtcctgtt gagtcgctag
     9241 aacaaggaga tcggttgggt gatgttctaa ccactccacg acggatttaa cgggatcctt
     9301 atgttggatc gttaactttc taacatttat ccccaattcc ggtactgctg accgtgggct
     9361 attcctcggt aacagttccc atcgttcaag tatatttctg actccaggaa actctgtcca
     9421 attcgcctcc atatcgtcgg cgacgtgcaa aatcgaaagc tttgctttgg cagttaaggc
     9481 tgctttaaga gcgtgggcga aagccgtatg gctgcctggg ctaaaatcgg aacaatgaac
     9541 cacagatcgg ataagcggtt gtcgtaaacg atggttgggc atgaaactcc tttcacgata
     9601 gacctgcaac acttcggtcc acgatcataa gcggcacaat tgtacttcaa atgatcgttg
     9661 aagaataata gcggttcaac gggtattgtc aaattaacga catgagggca caatgaagcc
     9721 acttttcaga ggcgtggcag cacacagttc atgcccacac gacgtcctgc caaacttgga
     9781 acgcctgagt acgtaacaga caatggttga ccgctagcat gagatggcga caggacaatt
     9841 ttcatgagca agtgggtagc aatcgtcctc ttcattgtgg cagcgttcac tcagaccatt
     9901 ggcggtctgg cgtcgggtac ggcgaccagg acgctttgtt ccgattcgtg gtacagattg
     9961 atagaggaga aagtgccgac gagcgacgag caagggcacg gcccggacgt agggtccgac
    10021 gaatggaagt cggtgatcga gttcaagctc ggtattcggg acaagccgga tgttccgagt
    10081 cgtgacaccg aagcctggtg ccgacacatc gatcaaatgg ttttggagaa ccgcacgtca
    10141 tcgaaaagtg tcggggatcc cggcggagct gccaaggcgc aaggcccctc gttcgcttgt
    10201 atcaatgtta ttggcggcag catcgaggcg atgatttgca aggatgagga attatcggcg
    10261 ctcgatcgca agctgtccga tgtctacgct tctgcatcga aaaaagcgag caacgagcat
    10321 ccttctgtcc tcaagacaga gcaacgcggt tggatcaaag ggcgcgacga ctgttggaag
    10381 agcgaagaca cacgcggatg tgtccgggat gagtacacac gccgcatcgc cgaactgcag
    10441 gccaggtatc ggctggtacc ccacagtggg ccagttagtt tcctgtgcgg ggacacctcc
    10501 gccaatgagg tcgtcgccat gtttttccag acccagcctc caacgttgat tgccgaacgc
    10561 ggggatagcg tctcgctgat gttcttacag ccgagtggca gtgggactaa gtaccagggt
    10621 cgcaacgaga ccttttggga acatcaaggg gaagcgttaa tcacctgggg ttacggtgcg
    10681 ccgtcgatgc attgcaaaaa gacgtcgtga ggtgaatccg taccagctca attaaccgtc
    10741 accgaatatc acaaactgcc cacagcgttg tttttggtca gtatagcctg agccacctat
    10801 ccctgcacca gcaacctggg caacggcggc gtcaacgccg atccgattgc ctggcacggc
    10861 caacccttct caattcttat cacccttcct cccttgaggg tagtcttttt tcaagccggt
    10921 ttgaccgggc attcctatta aatcggattc ttcccttgcc ttccggccac ggttcattcc
    10981 gccaccattt ccttgctctt atttttatga attaataaac ttctaccttt agtataaaaa
    11041 gataaatatc aatgttagtg ccaaggatag gcaccatgaa tatagcaatc gatgagtccg
    11101 gtacgtttgt ttatacagat acggataact cctggaattg tgtcgtctca tatgtttacc
    11161 ctgaagtgca caatccccac atccaagagg aagtaagaaa actcgtacag cgtcatgcga
    11221 aggggggaca aaaagaagta aagttgagaa atctcagcga gagtgcttac gcgaacttcc
    11281 ttcgttgtct gcagaagtgg gacggggttc tttacgcggt agccaccaat gctgctgcca
    11341 acaccccgca agtcttagag tgtcatcaag aggaacaaac tcaaaagata ctcgaacacc
    11401 tggacaagat gatttatgag accggacgtg agggcgtgcg tcggctcgcc aagcgcgtta
    11461 aggccttgcc gcatcagctt tacgtgcaaa tgatctgcca agtccagctg gttattcaaa
    11521 ttctccatag tgcactatta ttctatgttc aaagacaccc tcctacatta aatcgattcc
    11581 gatggcggat tgatcaaaag aattcaagcc agacgagcta tgaagatgcc tattcccagg
    11641 tgttgccggc aattctacaa agtgcgtcaa tatgcaaacc aataccgatg cttaagggtg
    11701 aagactacca ttggttcgat cggttttatt ttccgaaagg tgaagtaccc acatatttgc
    11761 gtgatgtgta tggagtagaa atagttgatg ataacgagcg taagataaac atcgggcagg
    11821 tagttcacga ggatgtccgc ttcgtggact cgaagtcaga ctctggggta cagatcgccg
    11881 atctccttgc ctcaggattg cgtcggtgtt tacggggtca gtttacagac aataatatta
    11941 ttgcctcgtt aattgggggt cttatggttc aaagagagca taaccaaatt cctatccaac
    12001 taatttcgtt ggggaaagag gaaaaaaagg tggaaggcca agttctttca gcattacatg
    12061 cgatggccat tcagtcacga gcaatgattg tgcgtcaata acaaatggga cgaagcttac
    12121 aaagtctctc gaaaaagcga ataaagagcc ccacggcaaa ctactgaaac tagcaagtta
    12181 ataaactttg accttcgata gaaaaaatcg atgtcatgtt ttttgcctga actagggtta
    12241 ccaattctgg agcaaactca tctgagatga acgatatagt acagaggagt atgcctacgg
    12301 taaaattcca aacacattct tggaagacaa ttaccaggtt attcccaaag acaacgttct
    12361 gtgtctggct gaagtggagg gacgaaatgc tgtatttctt gcgaagcagg gctactccgt
    12421 aaccgctgtc gatggctccc aagtcggctt acagaaggcc aaaaaattgg ctgaagagca
    12481 tggcgtttct attgaattaa ttcatgctga cttggctaat tttgacatag gccaaaaccg
    12541 attggagggc attgtttcga ttttctgccc cttgccttcg gcgctccgaa aggagctgca
    12601 ttaaaaggtg gtcgcagggt taaaacctca tggtgtatac cttgttgagg cgtatacacc
    12661 ggatcagttg aagcatggca ccggcggtgg aaaatcggca gattcgatga ccacgaaaga
    12721 atcgttaagt cttgagttgg aaggcttgaa gtttagacat cttctcgagc ttgaaagaga
    12781 tgtcgtagag ggcatttatc acacaggact cggcgcggtg gttcaggcag tcgcatcaaa
    12841 agaaatataa cgatgtcttc cggttgaccc gcacggacaa gcaaagaaaa caacatgagc
    12901 agaatatccc aagaaagaac ggaaaaagcc ttgcaaccac tcgatgggaa ttcccaagca
    12961 aattcaccca cgctataagc cgtggtcttc tttcgtttga tagaacccaa caaggggatg
    13021 gaacgggcag tccgaaatgt cactgaagtt catccccgac gttcggcggc aatggggccc
    13081 aggagagcct gggagattct gaaggtgacg cgaaggtctc tctactgctt gaataaccgt
    13141 ttattggaag gatgacaggc tgagtgccga ctggtgaaag gaggaggagc catgatcaaa
    13201 cacttcgacc acatcaccat tgtggtaaga gatgtcgagg aggccaagca gtttttcggt
    13261 ctgttggggt tcaaggaaga caagtccgtc gtgatcgcag gcccgcagtt ctcgaactac
    13321 atgggggtgg acggtatcga agctgaacac atcaccctcg tgcttgccaa cgtctcacca
    13381 cgaacggagg tgcaactact caagtatcgc tccccggacc ctctcctgga tcccgccatt
    13441 cgcaatctca ccaagcttgg gtttaaccac gtctgcttcg ccgtggacga tctggaggcc
    13501 gaggtggcaa aacttcaagc ccacggcgtt cacctccgga acgaggttat ggacttccat
    13561 gaccgcaagc tggtctttct ttccggaccg gagggtatca ccgtggaact ggcggagtgg
    13621 gatcaaagcc gaagcaaacg ctgaaccagc c
//
LOCUS       DAORAQ010000066        13236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_776_length_13236_cov_66.368940, whole genome shotgun sequence.
ACCESSION   DAORAQ010000066 DAORAQ010000000
VERSION     DAORAQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_776_length_13236_cov_66.368940"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKM72_07790"
     CDS             <1..558
                     /locus_tag="PKM72_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60723.1"
                     /translation="EKVEALLADDIASGSVRTIYNPFFAMADNLMSCWAARNDMTQDF
                     ILINGDTLFESAVLQGLLQAPPMPITLVTDEKVCYDADDMKVIVRGTQLDRIGKTLPI
                     EQVNGESIGMIRFQGNGPKIFLSTLDQRIRKPEALKQWYLSLLDELAQQGHVSTFCIK
                     GLNWAEIDNPSDLEHAERLFKIHPS"
     gene            complement(628..1458)
                     /locus_tag="PKM72_07795"
     CDS             complement(628..1458)
                     /locus_tag="PKM72_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNP60724.1"
                     /translation="MELDYRSVTALVLAGSRSGRDPVAQMAGVSTKVLAQVGGEPMIS
                     RVLQTLDQSRTVGKRILCGPSWETIQNNPFLGTLIESGAVQWVEPQQGPSLSVARFLD
                     EQPQEFPILVTTADHALLTAEMVDYFLHAAQEKQMDVAVGLVPYSSVVASYPQSKRTV
                     TRLTGGGFCGCNLFVLCTPKAKRMVEFWSQVERERKHPIRLIRTLGGLALVRYLFGWL
                     SLAEALNRLGQKLNLQVQEVLLPFPEAAIDVDTPEDLALAEAILAKRQEDMEKGVAPS
                     "
     gene            complement(1486..2610)
                     /locus_tag="PKM72_07800"
     CDS             complement(1486..2610)
                     /locus_tag="PKM72_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60725.1"
                     /translation="MIRIEPVTDRAGLKEFIRLPWKLYHSDPHWIPPLLVERLHTFSQ
                     KNPYFQHARWQCWLAYRGQEPVGRISAQIDDLHLSRYQDRTGFFGLLEAEEESSIFHA
                     LFQAAETWLQTQGMTNVRGPFNLSINQECGLLVDGFDSPPMIMMGHAMPYYPYGVEQN
                     AYEKAQDLLAYRIGIHFSFPPGVSLFLKKAKTSMRIRPLRRKHLNEDLAIIKDIFEEA
                     WSDNWGFLPFTDAEFAEIGQQLKLLVEDDFVQIAEIDGEPTAMLVAFPNMHEVIRDLN
                     GHLFPWGWLKILWRLKASYPRSGRVALMGVRKRFQSSPMGAAMAYGLIEAVREAGLRK
                     QIQHVEMSWILENNKRMRHILESLGAEPYKTYRIYEKHLQ"
     gene            complement(2710..3444)
                     /locus_tag="PKM72_07805"
     CDS             complement(2710..3444)
                     /locus_tag="PKM72_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNP60726.1"
                     /translation="MLTSQWYREKYLLDRMTFRDLIWAYVLYPSIQLYGVLLACSIVG
                     AIVIFEYFPVSPLRMAGSIVLTLLAYPFVEYAVHRFILHGRYLYRSPWTAALWKRIHY
                     DHHQDSNDLRVLFGAGSTTLPTIVVATFPIGMSVGGWVGAALSFGTGLACFMVYELCH
                     CMQHLRVNPKVKFLRRIKRHHLLHHFHNEQGNFGITSLFCDRIFGSEYGSAEDVPFSQ
                     TVSNLGYADEERSHYPWVAQLSEQKP"
     gene            3827..4270
                     /locus_tag="PKM72_07810"
     CDS             3827..4270
                     /locus_tag="PKM72_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60727.1"
                     /translation="MNTTMKKFGYPDTCLKDFSQWVVLLRPQQVTLGSLVLICREEAT
                     AFSQISADAFKELPGIIREIETSVSRTFGYSKINYLMLMMVDPEVHFHVIPRYDTPRL
                     FSQQQFFDHGWPGPPQLKNSNDTSEEVHQTIFSHLKNNWIRGTFI"
     gene            4488..6251
                     /locus_tag="PKM72_07815"
     CDS             4488..6251
                     /locus_tag="PKM72_07815"
                     /inference="COORDINATES: protein motif:HMM:NF006624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HNP60728.1"
                     /translation="MNATPTHHQLRLKHGNFSTLSEALDYAASGETGCNFYSGRGKLT
                     HVLTYAGLQQQAQNLARRLNGLELARGARVALIADTTPDFIRFFFACQYAGLVPVPLP
                     ISLHLGGHQAYVTQLKRLLESCQASIAMAPEGFLPFLEEAAAEMDLALIGGPETFASL
                     QETHDPLRPLEPHELAYIQYTSGSTRWPRGVEVTQSAVMTNLSGIIQHGLQVQTGDRC
                     VSWLPFYHDMGLVGFVLGPIASQLSVDYLSTRDFAMRPRQWLSLMTKSRATISFSPSF
                     GYELCVRRLGEDFNEQYDLSSWRIAGAGAETIRADSLSRFAKTLKASGFKSEAFLACY
                     GMAECSLAVSFAPLNKGLEVDRVDGDQLSNNRLAMPFVPSSTDDNQARCSKFVNCGVP
                     LPGYEVQIRNMNGDVLDERESGVIFVRGASTMTGYFNDPIMTEEVLSHDGWLNTGDIG
                     YWVNNSLVIIGRSKDMIIINGRNIWPQDLEFMAEQQAEVRPGDSSAFSVSSPDGHEIA
                     IMVVQCREMDSQKREDLVQRLRSLVHKELSIPCYIELIPPRTLPRTSSGKLSRSRAKA
                     DFLTRIQWEEVTPMLSIARSA"
     gene            6286..7230
                     /locus_tag="PKM72_07820"
     CDS             6286..7230
                     /locus_tag="PKM72_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNP60729.1"
                     /translation="MGTTVALTGASGFIGGVIAQHLKKAGWHVRGLARSPRQVQKLEN
                     LGIEPVQCRLENPASLLALVKDSFAVIHCAGAIRGISREDFYPTNVMGVSNIIQACLA
                     HSSPPRFVLLSSLAAREPSLSPYAWSKHEGEAVLTREAGDLKWSIFRPPAVYGPQDRA
                     LLPLFTILRKGIGIQLGPSQARFSLIHVDDLCNAILYWLKIQAPPSQIFEIDDGFPGG
                     YSWEDVFRISNPCLRLCLRIPPSLLQIGGKSNEIFSHLLRYTPLFTSGKAAELLHLNW
                     VCDNTQVAQTLGWTPQISLKEGLRRLFTSDSLMPSHRD"
     gene            7268..7513
                     /locus_tag="PKM72_07825"
     CDS             7268..7513
                     /locus_tag="PKM72_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNP60730.1"
                     /translation="MISYADILPQLFETLKPFAKENFRLKEQTELVAELGLDSLQVMQ
                     VLLKIEDHFDISIPLNNLPKIRTVKDLGEEIEKLLKG"
     gene            7516..8745
                     /locus_tag="PKM72_07830"
     CDS             7516..8745
                     /locus_tag="PKM72_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNP60731.1"
                     /translation="MSLFDKFLPIQEARNELLKSGVNPFHITVDKILSPTEAIINGRT
                     TILVGTNNYLGLTFHPDAILAGQQALAQEGTGTTGSRMANGSFASHRELEKAIAEFYG
                     APYAIVFSTGYQANLGVLSALAGAGDTILMDGDSHASIYDGCRLGGAEIIRFKHNDVA
                     DLEKRLRRLGDRSQQTLIVVEGIYSMLGDRAPLAEIVEVKKKYGATLIVDEAHSLGIL
                     GQHGRGLAEETGVEQDVDCIVGTFSKSLGSTGGFGISSHPEFELIRYASRPYIFTASP
                     CPSVVASTRATIRIIQSHPELRQQLWTNSQDLYNGLIKSGYTLGAEVGPIMAAMIPTR
                     ELALAMWQQLLEYGIYVNLMIPPATPNGECLLRLSVSAAHTKKQIEKIISAFAELSHI
                     TPTHTNPAQVQPDAILN"
     gene            8960..11227
                     /locus_tag="PKM72_07835"
     CDS             8960..11227
                     /locus_tag="PKM72_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HNP60732.1"
                     /translation="MWSIEQVRDPGRVYEIFLMKELSGFDRRKWLTGFTNYRFAIPSF
                     AGQVGRAIYNDVDQIYLADPGELFDHDLNDHGFLSIAQNDSSVMLMDCAKMAKVWSLN
                     AAKFERKNALLERALNVPNLWGQLEPEWNARDEEYQTGRSKVLHFTALHTQPWQPFPE
                     EFVYQKSSVGDVWFNLEYSANQEGFLTFTQSNPSSLFTKFATQARDIQANIPIQDTET
                     VSEIEEAIHILRPKTLLYCHLGEPVHRIPEHLKSGGLSPLAMTTLDLCHPDSKDLGFQ
                     KFDIVLAPKGLQLIPDDDIPWVIEALFHRAKCMVYVGIESTYPLQSFTAAPLQSDLTR
                     DFNWWISHIDSISARYPAIHWKVVFHTHSSKGITQSQIRCGGNWIGTLPRIWILSDGK
                     TGHTTQSELLAKSLGWPYDIKQLRFYGWHKIQKFLWGLAPPNIIGLDQRHSSPLQAPW
                     PDVVISTGWRPAPIARWIRDQSQGRTRIIQLGRKGGGPADLFDVVITPTYYGFSPHPN
                     RIETLAPLNGLTQEDLETVSQRWPDLFGNAPHPRIVFVVGGSTARFKFTPDMARMIGN
                     QLKDLAETCHGKIFAVTSPRTGKDLSQALEETLTPDHVVHTWQPNQSDNPYFAYLAGA
                     DVLIVTGESESMLAEAASLGKPVYIVPLPEHSPTWKVRLSEKIVQRAHFQPLNKRGTA
                     RPQQGIERLCARLIRSGLIQPRRNLSMLHDSLFKNGVARPFGDPIENGKRPQLNETGD
                     VAKKVKKALGFFDQS"
     gene            11246..12646
                     /locus_tag="PKM72_07840"
     CDS             11246..12646
                     /locus_tag="PKM72_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60733.1"
                     /translation="MSRIPKPRRQLTIPVEVFNREFDAKVLLSCFAAERGFSVIIGEK
                     HEIQLNLASLPRSIYLPTNLHNRNAIVFSLLAQLGHALVGTDEEAIVYCSPEVYVKEK
                     LGSTEFTRPNFFLAWGTENARIWKSQPQYNGMPIHITGNSRVDLLRPELRQYWKPEVE
                     SIHKKFGKIILINTNFGKLNHFRPNKGAEKEALERATVSPSDVDESELGMARHRLALF
                     QHFQDMVGQMAAAFPAHTILIRPHPSESHETWRQAAKGYPNVQVHIEGHVIPWLLAAD
                     AIIHNSCTTGLEGYVLKRPVFAFQPIMSDRFDKKLPNAVSRRATSSPELIELVKTTLS
                     GNQTAQEHDSEAQTRLIEQYLAALQGPFASENIVNTLETFDTTYLPPTPKLGTLLAAK
                     SQALGRKLRRRFNAQFRMSDQNRSQRYNYLNHIFPDINLAQIEDRIKRLQHALGRFHH
                     TQVRPLHDNIFEIFPK"
     gene            12741..>13236
                     /locus_tag="PKM72_07845"
     CDS             12741..>13236
                     /locus_tag="PKM72_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNP60734.1"
                     /translation="MYNVKIFGAGSIGNHLAHASRSLGWNVTICDVDQAALDRTKQTI
                     YPTRYGKWDEAVKLSLVKNAPRGGFDLIVIGTPPDSHLSLALGALEEKPKAILVEKPL
                     CSPDLEQGQTLQNMVTDSGTFAFVGYDHVVGKAAQETLQAGRQLSTIETIDVEFREHW
                     GGIFA"
BASE COUNT         3565 a         3451 c         2939 g         3281 t
ORIGIN      
        1 gagaaggtcg aagctctttt ggctgacgat atcgcctcgg gttctgtccg tacgatctat
       61 aatccatttt ttgccatggc agataatttg atgagttgtt gggcggcccg aaacgacatg
      121 acccaggatt ttatattaat taatggggac accctatttg aatcagcggt gctacaaggt
      181 ctgctgcagg ccccccctat gcccattacc ttagtaaccg atgagaaagt ttgctacgat
      241 gctgacgaca tgaaagtcat cgtccgtggc acacaattgg atcgaatcgg caaaaccctg
      301 ccaatcgaac aggttaacgg agagtccatc ggcatgatcc gttttcaggg caatggacct
      361 aaaatctttc tatcaacact agaccaacga attcgtaaac cggaggccct caagcaatgg
      421 tacttatcac tcctcgacga attggcccaa cagggtcatg tgtccacttt ttgcattaag
      481 gggttgaatt gggctgagat cgataatcca tccgacttag aacatgctga acgtctgttc
      541 aaaattcatc cctcgtgaaa tggggctcca acgattattt acgttaggtg attgtcgagg
      601 aagacaccca tgcccattcc tccatgatta gctaggagcc acgcctttct ccatgtcttc
      661 ctgtcgtttc gccaagatgg cttccgccaa agcaagatct tcaggagtgt ctacatcaat
      721 agccgcttcg ggaaacggaa gcagcacttc ttgcacctga agattgagct tttgcccaag
      781 tcgattcaag gcttccgcaa gcgacaacca accaaataga tagcggacca gggccagccc
      841 tccaagagtt cgaatcaggc gaatgggatg cttgcgctct cgctcaacct ggctccaaaa
      901 ttccaccatt cgcttagcct ttggggtgca gagaacaaaa agattacacc cgcaaaaccc
      961 tccgcccgtg agtcgggtaa ccgttcgctt agattgaggg tatgacgcca caactgagga
     1021 atacggcacc agccccacgg ccacatccat ctgtttttcc tgtgccgcat ggagaaaata
     1081 atcaaccatt tctgccgtta acaaggcatg gtccgccgtc gtgaccagaa tagggaattc
     1141 ctgaggctgt tcatccagaa accttgcaac actcagacta ggaccttgtt gaggctccac
     1201 ccactgtacc gctcctgact caatcaatgt tcctagaaac ggattatttt gtattgtttc
     1261 ccatgaaggc ccgcataata ttcttttccc cactgtgcgc gactggtcta acgtctgcaa
     1321 tacacgggaa atcatgggtt ccccacccac ctgtgccagg accttagttg atacccctgc
     1381 catctgggca accggatctc gcccggatcg ggaaccagca agcaccaggg ccgtcacaga
     1441 tcggtagtca agctccatat ggcttcacga aacacatatc tgcattcatt gaagatgctt
     1501 ctcgtaaatt ctatacgtct tgtatggttc ggctccgagg gattctagaa tgtgacgcat
     1561 gcgtttatta ttttctaaaa tccaggacat ttcgacatgc tgaatttgtt ttcgaagccc
     1621 tgcttccctc accgcctcaa ttaatccata ggccatcgcc gcacccatcg gacttgattg
     1681 aaatcgttta cgcacgccca ttaaggcaac ccgacccgac cggggatagg acgctttcaa
     1741 ccgccataag attttcagcc aaccccatgg aaatagatgc ccattcagat ctcgaatgac
     1801 ctcatgcata ttgggaaagg caaccaacat cgccgttggt tcaccatcga tttcagcgat
     1861 ctggacaaaa tcatcttcaa ccaatagctt aagctgctgt ccgatctcag caaattctgc
     1921 atcggtaaac ggaagaaatc cccaattatc tgaccaggcc tcttcgaaaa tatccttaat
     1981 aatcgctaaa tcttcattca gatgcttccg ccgtaacgga cgaattcgca tcgaggtttt
     2041 cgcttttttg agaaacagag aaactccagg gggaaaacta aaatgtattc caatacgata
     2101 ggccagaaga tcttgggcct tttcataggc attttgttca acaccgtatg ggtagtaagg
     2161 catggcgtgg cccatcataa tcatgggcgg tgaatcaaac ccatcaacca acaaaccaca
     2221 ttcctgattg atcgacaggt taaacggccc acgaacgttc gtcatcccct gagtctgaag
     2281 ccaggtttcc gcagcctgaa atagcgcgtg aaagatactg gactcctcct ctgcttcaag
     2341 caggccaaaa aaccccgtcc gatcctgata acgactgaga tgcaggtcat caatctgggc
     2401 agaaattcga ccgacgggtt cctgtcctcg ataagccagc caacattgcc aacgagcatg
     2461 ttgaaaatag ggatttttct gggaaaaggt atggagccgt tcgaccaata aaggcggaat
     2521 ccaatgagga tctgagtggt acaacttcca gggaaggcga ataaactctt tgagaccggc
     2581 gcggtcggtg accggttcaa tgcgaatcaa aacgcctttc cccagccatc ttggcgcacc
     2641 cttcgcctac aggaagtgcg gcctcgtgag gcgatgcgag ggtcactact acgaatcttc
     2701 atgggccttt tatggcttct gttcggatag ttgggccacc caagggtagt gggatcgttc
     2761 ctcatccgca taacccaggt tggaaacggt ctgactgaac ggcacatcct cagcactccc
     2821 atactcacta ccgaaaatcc ggtcacaaaa cagactcgtg atcccaaagt ttccttgttc
     2881 gttatgaaaa tgatgcagca aatgatgtcg cttgatccgc cgtaaaaact taactttggg
     2941 attcacgcgc agatgctgca tgcaatgaca gagttcatac accataaaac acgccaatcc
     3001 tgtcccaaaa gaaagggcgg cccccaccca gcctccaaca ctcatcccaa tagggaaagt
     3061 ggccacgaca atcgtaggta gtgtggtcga gcccgccccg aacaacaccc gtaaatcatt
     3121 ggaatcttgg tggtgatcat agtgaattcg tttccacaag gctgccgtcc atggcgaccg
     3181 atacaggtat cgcccatgta aaataaaccg atggacagca tattctacga agggataagc
     3241 aagcagggtc agaacaatac ttcctgccat gcgaagggga gaaacaggaa agtactcgaa
     3301 aatgacgatg gcaccgacga tactacaggc caataacacc ccatataatt gaatactcgg
     3361 gtataaaacg tacgcccata tcagatcgcg aaacgtcatg cgatcaagga gatacttttc
     3421 acgataccat tgactggtta gcaccctgga acctccatcc ctttcctcat ttgcctgttg
     3481 ccctcaatcc atcccacacc tgattcttca ggatcatccc ctatgacaat tatagaaacc
     3541 ttccgaagaa gccacaagaa tagacaactg gaaaccgata cctccaatcg aaaagcctga
     3601 atgattataa cggaatgcat taattcccac actcaaaaac ctggttcaat ccattacagg
     3661 atcaatgcct aacatagctg catagtaata ttgaaaccgg gtttaacata aaacccgtga
     3721 tatatttcga tgggccactc gggatgccaa tataggccca tggggacaga agagactctt
     3781 tgcctcatag acacaagaat atctctttgg gtataagtgc aggtccatga acaccacaat
     3841 gaaaaaattt gggtatcccg atacctgtct caaagatttt tcccaatggg ttgtgctcct
     3901 acgcccccaa caggtgaccc ttggctccct ggtattaatt tgtcgagagg aggccacagc
     3961 gttttcccaa attagtgccg atgcatttaa agaactgcct gggatcatac gtgagattga
     4021 gacaagtgtc tcacgcacat ttggctatag caaaataaat taccttatgt tgatgatggt
     4081 tgaccccgag gttcattttc atgtcattcc ccggtatgac acgcctcgat tattttctca
     4141 gcagcaattt tttgaccatg gatggcctgg gccacctcaa cttaagaatt caaatgacac
     4201 ctcagaagag gtgcaccaaa caattttcag tcacctcaaa aacaactgga ttcgcggaac
     4261 ttttatctag ctacaattac ctatataccg gaaactgact gttgcaaaat agactcaaat
     4321 tgcggttttt ttactacaat ttaaaccgaa taggaaaatt cactttcact tttccttcgt
     4381 tcttaatcat taatcaaaaa atcatcatgg cactataatt gcttaattaa taaaatgctt
     4441 tatagcaaac catttggaat aatattgcct ttaatccttg agatagaatg aacgctacac
     4501 caactcatca ccaactcaga cttaaacatg ggaacttctc caccctttca gaggcccttg
     4561 attatgcggc ttcaggagaa accggctgta atttttattc agggagaggc aaactcaccc
     4621 acgtgttgac atatgccggc ctgcaacaac aagctcaaaa tctagccaga agactgaacg
     4681 gattagaact agctcgtggc gctcgggtgg ccctcattgc tgacaccacc ccggatttca
     4741 ttcgattctt tttcgcctgt cagtatgccg gacttgttcc ggttcctctc cctatttcgc
     4801 tgcatttagg gggacatcag gcatacgtca cccaacttaa acggctactc gagagctgcc
     4861 aagcctctat cgccatggca cctgaaggct ttttgccatt tttagaggaa gcagcagccg
     4921 agatggatct agccctcatc ggaggcccag aaacattcgc ctcattgcaa gaaactcatg
     4981 atccgctcag accactggag ccgcacgaat tagcctatat tcaatacacc tctggtagca
     5041 cacgttggcc tagaggcgtt gaagtcactc aatcggccgt gatgaccaat ctatccggaa
     5101 tcattcagca tggcttacaa gttcagactg gcgaccgttg tgtctcctgg cttccctttt
     5161 accatgatat gggactggtc ggattcgttc ttggtccaat cgcttcacag ctttctgttg
     5221 attatttgag cacacgcgat tttgccatgc gtcctcgtca gtggctgagt ttaatgacca
     5281 agagtcgggc aacaatttca ttcagccctt cctttggata cgagctctgc gtgcgaagac
     5341 ttggggaaga tttcaatgaa cagtatgatc tcagctcctg gcgaatcgct ggcgctggag
     5401 cagaaacaat tcgtgccgat agcctctccc gttttgccaa aaccctgaag gccagcgggt
     5461 ttaaatctga agcatttttg gcttgctatg ggatggctga atgttctttg gcagttagtt
     5521 tcgctccatt gaacaaaggc ctcgaggttg acagggttga cggagatcag ctctcgaata
     5581 accggttagc catgcctttt gtcccatcct ccactgatga caaccaggct cgatgcagca
     5641 aatttgtgaa ttgcggtgtt cctctccctg gatatgaagt tcaaatacgt aatatgaacg
     5701 gagatgtcct cgatgagcga gaaagtggag taatttttgt gcgtggggca agcaccatga
     5761 ctggatattt caatgatcca atcatgacgg aagaggtcct ctcccacgat ggctggctca
     5821 atacgggcga catcggctat tgggtcaaca acagtctcgt cattattggc cggtccaaag
     5881 atatgatcat cattaatgga cggaatatct ggcctcaaga tttagaattc atggcagaac
     5941 aacaggcgga agtccgacct ggcgattcat ccgctttttc cgtttcctcc cctgacggtc
     6001 atgagatcgc catcatggtt gtgcaatgcc gtgaaatgga ttctcaaaaa cgggaggact
     6061 tggttcagcg cctacgatcc ttagttcata aggagttgtc tattccctgc tatattgaac
     6121 ttattccccc caggacactc ccgcgaacat catctggaaa actttctcga tctcgagcca
     6181 aagcagactt tctcactcga attcagtggg aagaggtcac tccaatgctg tccatagcca
     6241 ggtctgctta agaccggact atcctttcct cactcgtcct ccactatggg tactaccgtc
     6301 gccctaacag gggcttccgg ttttattggg ggagtcattg ctcagcattt aaagaaagct
     6361 gggtggcatg tccgtggttt ggcccgctct ccacgccaag ttcaaaagct cgaaaatctc
     6421 ggaatagaac cggtccaatg ccggttggaa aatcctgcca gccttttagc cctggttaaa
     6481 gactcgtttg ccgtcattca ttgtgcaggt gcgattcgcg gaattagccg agaggacttc
     6541 tatccgacca atgtgatggg agtctctaat atcattcaag cctgtctcgc gcattcctct
     6601 cccccacgat ttgttcttct ctcatccctc gccgctcgtg agccatcact ttcaccctat
     6661 gcctggagca aacatgaagg tgaagcagtt ttaacccgtg aggcggggga cctgaagtgg
     6721 agtatttttc gtccacctgc cgtctatgga ccgcaagacc gtgccctgct cccacttttt
     6781 accatattac gcaaaggaat agggattcaa cttggtcctt cccaagccag attttccttg
     6841 attcatgttg atgatctctg caacgctatt ctttattggc tgaaaatcca ggccccaccc
     6901 tctcagattt ttgagattga tgatggcttc cctgggggat attcctggga ggatgtcttt
     6961 cgtatttcca atccttgcct ccgcctgtgc ttgcgaattc caccaagcct cctacaaatt
     7021 ggaggaaaaa gtaatgaaat attttcccac ctcttgagat atactcctct tttcaccagc
     7081 gggaaggcgg ctgaacttct ccacctcaat tgggtctgtg acaataccca ggtggctcag
     7141 accctcggct ggactcctca aatctcactc aaagaaggct tgcgtcgatt gttcacatcg
     7201 gatagtctca tgccatccca tagggattag tacagatccg tcctcctccc tcatcaagaa
     7261 ggctattatg ataagttatg ctgacattct cccccaactg ttcgaaaccc ttaagccgtt
     7321 tgccaaagag aactttcggt taaaagaaca aacagagctg gttgccgagt tgggattaga
     7381 ttccttgcag gtcatgcaag ttctcttgaa aatcgaggat cactttgata tttccatccc
     7441 cctgaacaat cttcccaaaa tccgaacagt gaaggaccta ggcgaagaga ttgagaaatt
     7501 gctgaaaggg taatcatgtc attattcgac aaatttcttc ctattcaaga agccagaaat
     7561 gagttgctaa aaagtggagt caacccgttt catatcactg tcgacaagat cctttcacca
     7621 acagaggcca tcatcaacgg gcgaacgact attctggttg gcacgaataa ctacttaggt
     7681 ctaacctttc accctgacgc cattctggcc ggtcaacagg ctcttgctca ggaagggacc
     7741 ggcacaacgg gatcgaggat ggcgaatgga tcttttgcga gccaccgtga gcttgagaag
     7801 gccatcgcgg aattctatgg tgcgccttat gcaatcgttt tttccacggg gtaccaagct
     7861 aatcttggcg tcctttctgc tcttgcaggc gcgggggata ccatattaat ggatggggac
     7921 tctcatgcca gcatttacga tggatgcagg cttggagggg cagagatcat ccggtttaag
     7981 cataatgacg tggcagactt agaaaaacgt ctccgtcgat tgggggacag aagtcagcag
     8041 accttaattg tcgtcgaggg catttatagc atgttgggag atcgagctcc tctggccgag
     8101 atcgtcgaag taaagaaaaa gtatggagcc acactgatcg ttgacgaagc ccattcgttg
     8161 ggcatactcg gccaacatgg acgaggcttg gcggaagaaa caggcgtcga acaggacgtc
     8221 gattgcatcg tcgggacgtt cagtaaaagc ctaggctcaa caggaggatt cggtataagt
     8281 tctcatccag agttcgagtt aatccgctat gccagtcgtc cctatatttt tacggcctcc
     8341 ccatgtccat ctgttgtcgc ttccacacgg gcaaccattc ggattatcca gtctcacccc
     8401 gaacttcgcc aacagctatg gacgaattca caggatttgt ataatggatt aataaagagt
     8461 gggtatacgc ttggggcaga ggtcggaccg attatggcag caatgatccc taccagagaa
     8521 ttggctcttg ctatgtggca acaactcttg gaatatggca tttatgtcaa cctcatgatt
     8581 cctcctgcca caccaaacgg cgaatgcctc cttcgattaa gtgtcagcgc agcccatacg
     8641 aagaaacaaa ttgaaaaaat tatttctgca tttgcagagt tgtctcatat cactccaacc
     8701 cacaccaacc ctgctcaggt gcaaccggat gcaattctca attaaacaat gtttctgttt
     8761 cgatcacatg tcgaaataac cacctaaacc tatttccaaa cctcccatga cacgccaccg
     8821 ctctagacca aacggcatta gggcaattcc cgaaaaaata accttggaag ccagagaagg
     8881 acatacctcc tccgggaaat ctccagtcag aatttttatt ggaagtgaat catcccaata
     8941 cagggctgaa cgcatcctcg tgtggtccat tgaacaggtt cgtgatccag gtcgagtcta
     9001 cgaaattttt ctcatgaagg aattatcagg atttgacaga cggaaatggc tcacagggtt
     9061 tacgaattat cgatttgcga ttccatcatt tgcaggccag gtcggacgag cgatttacaa
     9121 cgatgtagac caaatctacc tggccgatcc tggggaatta ttcgaccacg acttgaacga
     9181 tcatggcttt ctctctattg ctcaaaatga ttcctccgtc atgctgatgg attgcgctaa
     9241 aatggcaaag gtctggtccc ttaatgcagc caaatttgaa agaaaaaatg cactattgga
     9301 acgcgcctta aatgtaccca acctctgggg acaattggaa cccgaatgga atgcccgcga
     9361 cgaggaatat caaacagggc ggtcaaaagt cctccatttt actgctcttc atacgcaacc
     9421 ctggcagcct ttccccgagg aatttgtgta ccagaaaagc tcggttggag atgtgtggtt
     9481 caacttggaa tacagcgcga atcaagaagg gttcctgacc ttcacccaat ccaatccatc
     9541 ttcattattt accaaattcg ccactcaagc acgcgatatc caggccaaca tccctatcca
     9601 ggataccgag accgtatccg agatagaaga agcaatccac attctcaggc ccaaaactct
     9661 tctctattgt catctcgggg aacctgttca ccgcattccg gaacatctga agtccggcgg
     9721 cctttcccca ttagccatga cgactcttga cctttgtcat ccagactcta aggatctagg
     9781 atttcaaaaa ttcgacattg ttcttgcacc caaaggactg caactcattc cggatgacga
     9841 tattccatgg gtcatcgaag ccctgtttca tcgcgcaaaa tgtatggtct atgtgggaat
     9901 agaaagcacg tatccactcc agtccttcac ggccgcccct cttcaatcag atttaacacg
     9961 agatttcaac tggtggatca gtcatattga ctcaatcagt gcccgctatc cggctattca
    10021 ctggaaagtc gtgttccaca cccactcctc aaagggaata acacagagtc aaatccgttg
    10081 tggaggcaat tggattggga cgcttccgcg aatctggata ctctcagacg gcaaaactgg
    10141 acatacgacc caatcagaat tactcgcaaa gtcgttgggg tggccttatg atattaaaca
    10201 gttacgattc tacgggtggc ataaaattca aaaattcctg tgggggttgg ccccaccgaa
    10261 tattataggg ttagatcaac gtcactcttc ccccctccag gccccttggc ctgacgtggt
    10321 aatctcgacg ggatggcgac cggcaccaat tgccagatgg attcgtgacc agagccaggg
    10381 aagaacacga atcattcaat taggtcggaa gggaggaggt cctgctgatt tatttgatgt
    10441 cgtgattaca ccaacctatt atggattttc gccgcatccg aaccgtattg agacactcgc
    10501 cccgttgaac ggattgacac aagaagactt ggagaccgtc agccaacgtt ggccggacct
    10561 ctttggcaac gctccccatc ctcgcatagt ctttgtcgtg ggaggttcca ccgcacggtt
    10621 caaatttact ccggatatgg ctcgcatgat tgggaaccaa ctcaaagact tggccgaaac
    10681 atgccatggg aagatttttg ctgtcacatc ccccagaacg ggtaaggatt tgagtcaagc
    10741 ccttgaggaa actctgactc ctgaccatgt tgttcataca tggcaaccaa accaatccga
    10801 caatccgtat tttgcgtatt tagctggcgc agacgtgcta attgtcacag gagaaagcga
    10861 atcgatgttg gccgaagccg catctctggg caagcccgtc tatatcgtcc cacttcctga
    10921 acattcacca acgtggaaag tgcgcctcag tgaaaagatt gtacaacgtg cgcacttcca
    10981 acccctcaat aaaaggggaa ccgcccgtcc ccaacaaggg attgaacggc tttgcgcccg
    11041 cctgattcgt tcggggttga ttcagcctcg acgaaatctc agcatgttgc atgattccct
    11101 tttcaaaaat ggagtggctc gaccatttgg cgaccccata gaaaacggga agcgcccaca
    11161 attaaacgaa accggggacg tggctaaaaa agtcaaaaag gccttggggt tttttgatca
    11221 gtcttgaaaa tctacggaaa ttatattgtc acgcatacca aaaccccgtc gacaactgac
    11281 tatccccgtt gaagttttca acagggaatt tgacgccaaa gttttgcttt cctgcttcgc
    11341 cgcagaacgg ggattttccg tcattattgg cgaaaagcat gaaattcaac tcaatctggc
    11401 ctctttaccc agatccatct atctccccac aaaccttcat aaccggaatg ctattgtctt
    11461 ctcccttctg gctcaattgg ggcacgcgct ggtaggaacg gacgaggagg cgattgtgta
    11521 ctgttctccg gaagtgtatg tgaaagaaaa attgggctcg acagaattta cccgaccaaa
    11581 tttcttttta gcatggggaa cagagaatgc cagaatctgg aagtcacagc cccaatacaa
    11641 cgggatgccc atccatataa ccggaaattc ccgtgtggat ttattgcgac cggaactgcg
    11701 ccaatattgg aagccggaag ttgaatccat ccataaaaaa ttcggaaaga tcatcctgat
    11761 caataccaat ttcggtaaat tgaaccactt caggcccaac aagggagctg aaaaagaggc
    11821 gcttgaacga gcaacggtct caccatctga cgtggacgaa tctgaacttg gaatggccag
    11881 gcaccgcctg gcgctatttc agcatttcca ggacatggta ggccaaatgg ccgcagcctt
    11941 ccccgcacac accatattga tccgtcccca cccctctgaa agccatgaga catggagaca
    12001 ggctgcaaaa ggatatccaa atgtccaggt tcacatagaa ggacatgtca ttccctggtt
    12061 attggcggcc gatgccatta ttcataatag ttgcaccacc ggcctggaag ggtatgtttt
    12121 gaaacgtccc gtttttgcct ttcagcccat catgtccgac cgttttgaca aaaaacttcc
    12181 gaatgccgtt agccgccgtg caacatcctc tccggaatta attgaactgg tcaaaacaac
    12241 cctttcaggt aaccagactg cacaggaaca tgactctgaa gcccaaaccc gtttaattga
    12301 acaatatctc gcggcccttc aagggccatt tgcctcagaa aacatagtga atacgctcga
    12361 aacattcgac accacctatt tacccccgac cccgaaacta ggcacgctat tggccgcaaa
    12421 atcccaggcg ctcggcagaa aattacggcg gcggttcaac gctcaatttc gtatgagcga
    12481 tcaaaaccga tcgcaacgct acaattatct gaaccacatt ttccctgaca tcaacctggc
    12541 gcagatcgaa gaccgcatca agcggttaca acatgcatta ggaagatttc atcacacaca
    12601 agtcaggccc ctgcacgaca atatttttga aatatttccc aaataaatac tcaggaatga
    12661 agaatcccta cacccgcaat tatgctgggc ataaatgtgc atccatatac cgagtctcgt
    12721 gaacattcta aggaggatac atgtataacg taaaaatttt tggagcgggc tccattggta
    12781 accatttggc tcatgcatcc cgctccctcg gttggaacgt cacgatatgc gatgtggacc
    12841 aagcagcatt ggatcggaca aaacagacaa tttatcccac tcgttatgga aaatgggacg
    12901 aggccgtcaa gctttccctg gtcaaaaacg ccccgagagg agggtttgac ctcattgtca
    12961 ttggaacgcc gccagattcc cacctctccc tggccctcgg ggctcttgag gaaaagccca
    13021 aagctattct tgtggaaaaa cctctctgct ccccagacct ggaacagggc caaacattgc
    13081 aaaatatggt aaccgattct ggcacattcg cgttcgtagg atatgaccac gtcgtgggaa
    13141 aggccgcgca ggaaacactt caagccggtc gccaattatc gaccattgaa accatcgacg
    13201 tggaatttcg tgaacattgg ggcggcatct tcgcgg
//
LOCUS       DAORAQ010000067        12927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_801_length_12927_cov_67.939636, whole genome shotgun sequence.
ACCESSION   DAORAQ010000067 DAORAQ010000000
VERSION     DAORAQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_801_length_12927_cov_67.939636"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..848
                     /locus_tag="PKM72_07850"
     CDS             75..848
                     /locus_tag="PKM72_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNP60735.1"
                     /translation="MLQSTFLFLPGIGESTERLWWGDGIGTWDAFLEKTIAPRISSFR
                     KPQYDEAILEAQKHWQAQNSRFFTHILKARDHWRLYPNFRSQAAFLDIETNGIPLPDG
                     EITVVGIYGKGRMTTFIRGENLSGERLQAEFASYDVLVTFFGSGFDLPFLKAKYPDLI
                     LDHPHIDLCFSARRLGLKGGLKAIETEIGCYRPASLEGLTGWDAVRLWEEWQLGQAAS
                     REILIRYNEADCKNLEPLADLIYNRLAQRHGIPEFIASL"
     gene            845..1624
                     /locus_tag="PKM72_07855"
     CDS             845..1624
                     /locus_tag="PKM72_07855"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNP60736.1"
                     /translation="MTSAQPSNKWTGIGLTHIGLVRKLNQDAFSIDNTLQLWVLADGM
                     GGHAGGELASQIAVKTIPDVIRTQRATETSEYVQPEKLESVLNQGLESANERIRREAA
                     ENERLKGMGTTIVVVAISRSPTGFQASVAHAGDSRAYLFRQGSLSLWTKDHTLMEERL
                     ALNLITPEQVRTHPLRHVLTKALGIDPEAQPTVQTYPLEPSDLILLCSDGLTKMLTDQ
                     EIQTIVSQEAPHAEAICRTLVGTANQLGGEDNTTVVLIGLH"
     gene            complement(1646..2386)
                     /locus_tag="PKM72_07860"
     CDS             complement(1646..2386)
                     /locus_tag="PKM72_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNP60737.1"
                     /translation="MPDPTVNDIDQLLTETIQTLKLEEVSRHYRDQGEFVALEHFMPT
                     QVVQEFMREVERVRPQINRNFIPGHKKGGSVSFYLLQQSAPAILAFYQHQGWINLLSQ
                     IAGVPLMLCPEEDPHSCALYFYTEAGDHIGYHYDTSYYKGERFTVLLGLQDQSSSRLV
                     CRLHTKEQGREVKELSLRTDPGTYIFFNGDKLHHAVTPLGAGEERIVLTLQYVTNPSM
                     GLAQRWFSNMKDAVGYFGWSAMFRKPHR"
     gene            complement(2507..2674)
                     /locus_tag="PKM72_07865"
     CDS             complement(2507..2674)
                     /locus_tag="PKM72_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60738.1"
                     /translation="MPYDIDDRGPLSIVDLDPLNRQILVFHQGKACSLDALDAGLNVP
                     QNVDQCEMRPK"
     gene            complement(2890..4620)
                     /gene="recN"
                     /locus_tag="PKM72_07870"
     CDS             complement(2890..4620)
                     /gene="recN"
                     /locus_tag="PKM72_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNP60739.1"
                     /translation="MLTGLRISNFALIDQLHLELPPGFLVLTGETGAGKSLLIDALVL
                     LTGGRASAEHIRFGADEALLEACFILPATHHLTIRLQRDGYLLPDQQDLIVRRLLSRS
                     GKNRNFLNGQLAPLQTIQDIGSQLVDIHGQHDQQSLLSAKTQLKLLDAFGNLEDVVGR
                     YQQMHREWFEKKAALDEYVGKIRDQANRQDILQYQYDELVKMQLQSGEEESLSQEYHR
                     LKHSGRLGELSNQAFTALHEGERSVLDHLGEVTERVQELAKIDAQGEPWVPLLEAATV
                     ALREVTDNLRDYRNQIEYDPERMDLIDSRLAGLQRLKKKYGKPIEDLVELTTILKQDL
                     ALLQNGEEQVAHLQAEVTSRYESMKASAEELSARRKTVAKHLVKEIKQELAALKMSTM
                     EVQVNIEMANGDTGIGLTGMDRVEMLLAPNPGEPLMPLGRIASGGELSRIMLALKTVF
                     AENDQTPVVIFDEIDSGIGGEAGMVMGSRLRQLAKFHQVCCITHLPQIASQAHAQFVV
                     EKTTLDDRTLTKVHEVTGVHRETEIARMLGGGTLTPTIRKTAGEMLDRGNSPHVGVGL
                     QKPRKKPAKT"
     gene            complement(4646..4993)
                     /gene="trxA"
                     /locus_tag="PKM72_07875"
     CDS             complement(4646..4993)
                     /gene="trxA"
                     /locus_tag="PKM72_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNP60740.1"
                     /translation="MSDLVAKADATNWDGEVLKSSEVVMVDFWAVWCGPCQMVAPIVD
                     ELAQEYQGKLKVMKLNTDDAPEVAGQYQIMSIPSILFFKGGQVVEKIVGARPKQQFKQ
                     IIDSLLAQSTSPA"
     gene            complement(5160..5720)
                     /locus_tag="PKM72_07880"
     CDS             complement(5160..5720)
                     /locus_tag="PKM72_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60741.1"
                     /translation="MRALSASKRAWELMVCFGVGVILLVTLPHSNGWAQSDQIPILTS
                     KPLAHAKVFLSAGDYRRAVEACQKNIDRNPSVEAYVYLAYVYQAIDGYLGSLAKQEDY
                     VKVEQLALNMTARDILDLIDPPNVMPRMAQELIHEGIRQQFDVTAAMANRLSRTQTEE
                     LWAQQTRWRELNPDSWWTGTPDEWQW"
     gene            6159..6728
                     /locus_tag="PKM72_07885"
     CDS             6159..6728
                     /locus_tag="PKM72_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HNP60742.1"
                     /translation="MKQNKRLLPFILIIGLETFFLCLIPVSPTHVNSADIALVLSSSS
                     PGSSLPLEMTQTKDSTHSDDTLAALTIYLEARGESFAGKLGVAAVIRNRMTHKYHSDG
                     TVKGTVLRAKQFEPWIGRNPNEVKFDQTNRKMQESLLAWRLVQDGRKVVNGAVLFYNP
                     KLVKTPHWAQVNRKVAQIGGHEFFNKHTI"
     gene            complement(6729..7607)
                     /locus_tag="PKM72_07890"
     CDS             complement(6729..7607)
                     /locus_tag="PKM72_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNP60743.1"
                     /translation="MDDDAQHFTEPAIGIKELDSSCLGSVSQDKAENGEAQDRLDQEH
                     VTRILLEFLPIIRRMAGVMAFKNPFSLDVEDLTSAGAMGLLSALKRYDPTRDIKFRTF
                     AEYRIRGMMLDEIRAMDWVPRSVRSRRDQVRQIVEEHLQKNGTPPTSQELAALLGVPI
                     DEIEGVGGCDPRLISLDEPVGQGEDECTLRDVLPDVNHLDPFVACADAEMKAALSAAL
                     ETLSARQQEVLQSYYHTGLTMKEIGHQMGLTESGVCRVHSGALRHLRIELKRIEDGGR
                     SATRPKNLRKTRAGND"
     regulatory      complement(7694..7784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8084..9172)
                     /gene="lptG"
                     /locus_tag="PKM72_07895"
     CDS             complement(8084..9172)
                     /gene="lptG"
                     /locus_tag="PKM72_07895"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNP60744.1"
                     /translation="MKKISWYVFGKFSRVFLMCLVTVLTIYLVVDFFEKLRKFLKYDA
                     DLTVIFSFFVYRIPEIAFLLAPLAALMASILTVGGLNRTREITAMRSCGLSFYQIAIP
                     FFVFGALVSVVGLSLTAVLIPLANIKAEYVQSVLIQNKPEPLSLTPEGLWLRVGRNHL
                     LRIDTVTEDGHRLNGIHQYTLDDRFNLKGILEGEWATFVDDEWVIEHVIQRTFQEEER
                     MQVNGMPRQVLRLSLTPDDFQNWLSQSPEHMSLYELHDYIQRVIEDGHSPHRFLTDYW
                     ARFAYSIVPLVMTLLGISVSLRGSGVRGVGVAKGLGQTLAIGFFFWAAHSVGIVLGRN
                     GAVLPIIGSWIATAMFLVIGINLFLKLK"
     gene            complement(9202..10290)
                     /locus_tag="PKM72_07900"
     CDS             complement(9202..10290)
                     /locus_tag="PKM72_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNP60745.1"
                     /translation="MLIRLLDRYIFFELLPPFLTSLTGLCFIIFTKEMLRLVDLVVSR
                     GISLAGLGSIVLHLLPSFLVLTLPIACLIASISAFNRLSFDNEVTAIRAAGVSFWRIN
                     LPVAVFSGMVFLLTVYLSQWGQPWAKVSLKTLALSVIEDELTLAVDSGVFNEPIPGLM
                     VYVPHSENGSGQKGVFISDQRNPDQPLIIVAENFQMLKQSERQQFGIRLFRGAIHQIP
                     KDANSFHRVAFDTYDFWLSSPFTSVKEKTKRKTYQDLTQQLEESGGTDAGALRRLIEF
                     HKDTAFPVATFVLGLLGVPVGIVSKRSGKAGGFAIGVLVVIGFYLLNVLGEFLVTTLV
                     ISPLAGAWMPNIILACLTGFLLFQARKQ"
     gene            10605..10826
                     /locus_tag="PKM72_07905"
     CDS             10605..10826
                     /locus_tag="PKM72_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNP60746.1"
                     /translation="MRSKGTVKWFNDRKGFGFIQVEGGQDVFVHYSALQEDGFKSLKE
                     GESVEFDLVEGAKGPQAANVTKSGIGQPS"
     gene            11118..>12927
                     /locus_tag="PKM72_07910"
     CDS             11118..>12927
                     /locus_tag="PKM72_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60747.1"
                     /translation="MTKLLDALFFENPLSRNRLLTVESGEEDWGAGQNQDANIPQAPD
                     NLRAIPDHTRITLTWDPVPEALYYNVYFMTTKGVQIKPNELTRPVAGHEDFIPKVGIT
                     KEKANCIEGAISPYEHNDLANNFCYHYAISVVTEEGESLLSEEVMSIPSPYLSVMQIG
                     CEGVDDGEFKSPTGIAIDKDGNIYVGDTDNHSIQKFDRSGKFLARWGDEPDSSEGKFY
                     YPRGIATSPEGEVYVADSGNNRIQKFDSEGNIINAWGKFGFAWRGAEAGKFDVPWGVT
                     TDSQGNLFVSDTSNARIQKFLPNGSPVLKWGRDGGYDGAFFFPRGLAVDFVGNIYIAD
                     EGNNRIQKFDARGNFLLKWGKEGNAPGQFKAPWGVACDPLGYVYIVDSGNHRVQKFDS
                     SGTYLTSWGNRGLTEGQLNFPSGIAVDKEGYVYVIDSGNHRLTKFAPTEEELNRGKQE
                     KRVSSDLTAPINLAVKPGDTENILSWLEVPNAVSYNLYFSTEPNVSTETSTKIEGVTT
                     PYIHSGLTNDTPYFYAMTSVTEEGAESQLSEEQTAIPVLIDISAPQNPDIVINHGAYM
                     TNSPDMVATISAKDVDSGVAAYFISENPMTPSGTMPG"
BASE COUNT         3576 a         3275 c         2809 g         3267 t
ORIGIN      
        1 aagattgctt cgaatgtagc ttgtcctctt ggtgaaatca aggttagaat taagcccatt
       61 tcctgtttag acacatgtta caatccacat ttcttttttt acctggtatt ggagaatcta
      121 ccgaacgtct ctggtggggg gatggtattg gcacatggga tgcttttctc gagaaaacta
      181 tcgctccccg catttcttcg tttcggaaac cgcagtacga tgaggccatt ctggaggcgc
      241 aaaaacactg gcaagcgcag aattcccgct tctttaccca catcctgaaa gcgcgcgacc
      301 actggcgtct ttatcctaac tttcgatctc aggctgcgtt tctggatatt gagacaaatg
      361 gaatcccatt gcccgatggc gagattacgg tggtgggtat ttacggaaaa ggtcgcatga
      421 caaccttcat tcggggagaa aacttatccg gggagcggtt gcaagccgaa tttgcctcct
      481 acgatgttct cgtaaccttc tttggctcag gttttgacct gccatttttg aaggccaaat
      541 acccagactt gattcttgac catcctcata tcgacttgtg tttttcggcc agacgccttg
      601 gtttaaaagg tggactgaag gcgattgaga cggaaatagg ctgctatcgc ccggcttcgc
      661 tggaaggact cacggggtgg gatgccgtcc gtctgtggga agaatggcaa ctcggacaag
      721 cggcctctag agagatactt atccgatata atgaagcaga ttgcaaaaat cttgagcctt
      781 tggccgacct tatttacaac cgtctggccc aacgccatgg aatcccggaa ttcattgctt
      841 ctctatgaca tcagcgcaac catctaacaa gtggactggc ataggactga cgcatattgg
      901 tctggtgaga aaactcaacc aggatgcgtt ttcgatcgac aacaccctac aactttgggt
      961 attggcagat ggaatgggcg gacatgctgg gggagaattg gccagccaaa ttgcggttaa
     1021 aactattccc gatgtcattc gaacccaacg ggcgactgaa acctctgaat acgttcaacc
     1081 tgaaaaatta gaatcggtgc tgaatcaagg ccttgagtct gcgaatgaaa ggattcgacg
     1141 ggaagccgca gaaaatgaac gactgaaagg aatggggaca accattgtcg tggtcgccat
     1201 cagtcgttcc ccaacgggat ttcaggccag tgtagctcat gccggcgaca gccgggcata
     1261 tcttttcaga cagggttcgc tctcgctctg gacaaaagat cataccctca tggaggagcg
     1321 tttggccctc aacctcatta cccctgaaca ggttcgcaca caccctcttc gtcatgtgtt
     1381 gaccaaagcc ctgggaatcg atcctgaagc acaacccacc gttcaaacct atccgcttga
     1441 accctcagac ctcattctgc tttgttcgga cgggctcacc aaaatgctga ctgatcagga
     1501 gattcaaacg attgtcagcc aggaagctcc gcacgctgaa gccatatgtc gtacacttgt
     1561 gggtactgcc aaccaattag gtggagaaga taatacaacc gtggtgttga ttgggttgca
     1621 ctgaggaagg atatggaacc agtctttaac ggtgaggttt tcgaaacatg gccgaccatc
     1681 caaaatatcc cacagcatct ttcatatttg agaaccagcg ttgagccagc cccattgagg
     1741 gattagtgac gtattggagc gtcagcacaa tccgctcttc cccagcccca agcggcgtca
     1801 cggcatgatg caatttatcg ccattaaaga aaatatatgt gccggggtca gtcctgaggg
     1861 atagttcttt aacctctcga ccctgttcct tggtatggag acggcagacc agtcgactgc
     1921 tcgattgatc ctgcaaaccc agtaataccg taaatcgttc acctttgtaa tatgaggtgt
     1981 cataatgata cccgatatgg tctcctgcct cggtataaaa atacagcgca caggaatggg
     2041 gatcctcttc agggcacagc attaacggaa ctccggcaat ctggctcagg agattaatcc
     2101 aaccctgatg ctgatagaac gcaagaattg cgggagctga ttgctgcaga agataaaagc
     2161 tgacacttcc gcccttttta tgccctggta taaaatttcg attgatctgg ggccgcacgc
     2221 gctctacttc ccgcataaac tcctgaacaa cctgagtggg catgaaatgc tccaaagcca
     2281 caaactcacc ctgatcccga tagtgacggg aaacctcctc aagcttgaga gtctggatcg
     2341 tctcagtcaa cagttgatca atgtcattta ccgttggatc gggcatatgc aatctccttc
     2401 attcctccac gctccaacat ttacgctgat ccattttttt aaaaccatca ttgaccctag
     2461 tttaacatgc ctttcaccac attctcacga aacgataata aaacaattac ttcggcctca
     2521 tctcacactg atcgacattt tgcggtacat tcaaccctgc atctaaggca tcgagtgaac
     2581 acgctttacc ttgatgaaag accagaatct gccggtttaa cggatcgagg tcgacgatac
     2641 taagaggacc gcggtcgtcg atgtcgtagg gcatggcgtt caccttttta tcgattggat
     2701 cttcccaacg aacgactttt catccattgt gccgacggga cttttagctg agactattcg
     2761 ttagcttcac aatgaatggt tatgaggttt ttgagaaacc cgaatcttaa caattctctc
     2821 agacagagaa taagttatcc acccgttcat gaatggctac ctgaatgtac cgggtcattc
     2881 aaaattagat taggtcttgg caggtttctt tcttggcttt tggagaccga cgcctacgtg
     2941 agggctgttt ccccgatcca gcatttcacc ggcggtcttt ctgatggttg gggtcagggt
     3001 gccaccaccc aacatgcgag cgatttcggt ttcccgatgt actccagtga cttcatgtac
     3061 tttcgtcagc gttcggtcat ctagcgttgt tttctccacc acaaattgtg catgcgcctg
     3121 cgaggcaatc tgtgggagat gggtgataca acatacttga tgaaatttcg ccaattgacg
     3181 caaacgactc cccatgacca ttcccgcttc cccgcctata ccactgtcaa tttcatcgaa
     3241 aataacgacg ggagtctgat cattctccgc aaacacggtt tttaaggcca acatgattcg
     3301 agacaattct ccacccgatg caattcggcc caatggcatg agcggttctc ccgggttggg
     3361 agccaggagc atctcaacac gatccattcc tgtcaaacca attccggtat ctccattggc
     3421 catctcaata ttaacctgca cctccattgt cgacattttc agagccgcta attcctgctt
     3481 gatctccttg accaaatgct tcgcaactgt tttccgcctg gccgataatt cttcagccga
     3541 ggccttcatc gattcatatc gactggtcac ttctgcttgc aaatgcgcca cttgctcttc
     3601 tccattttgg agtaacgcca agtcttgctt caggatagtg gtaagttcca ctaaatcttc
     3661 gatcggcttt ccatatttct ttttaagccg ctgaagaccg gccaaccgac tatcaatcag
     3721 atccattcgc tccgggtcat actctatctg attccgataa tctcgaagat tatccgtcac
     3781 ctcccgcaac gccacggtcg ctgcttccaa caatgggacc catggttcac cttgcgcatc
     3841 tattttcgca agttcttgta cccgctcagt cacctcaccc aggtgatcca gaacagatcg
     3901 ttctccttca tgtaacgcag taaaagcttg attcgataac tctcctaatc gaccgctatg
     3961 tttcaatcga tggtattcct ggcttagaga ctcttcttct ccagactgca actgcatctt
     4021 gaccaactcg tcatattggt attgaaggat atcctgccta ttggcttgat cccgtatctt
     4081 ccctacgtat tcatccagag ccgccttttt ctcaaaccat tcacgatgca tctgttgata
     4141 gcgacctacg acatcctcaa gatttccaaa tgcatccagc aatttgagct gggtcttagc
     4201 ggaaagcagt gattgctggt catgttggcc atggatatct accaactggg aaccaatgtc
     4261 ttgaatcgtt tgaagagggg ccagttgccc attgaggaaa ttgcggttct ttcctgaccg
     4321 cgacaacaac cgacgaacga taaggtcctg ttgatccggc aggaggtatc cgtcccgttg
     4381 taacctgatc gtgagatgat gtgtcgcggg aagaataaaa caggcttcta acagggcttc
     4441 gtcagcacca aaccgaatat gctctgcaga ggctcgcccg ccggtaagta ataccagggc
     4501 atcaatgagg agagattttc cagctccggt ctcccccgtc aggacaagaa atccaggcgg
     4561 gagctccaga tgaagttgat caatgagagc aaaattggaa atacgcagcc cggtcagcat
     4621 aacaccgagt catcggcccc actgatcagg caggggaagt agattgagcc aggagcgagt
     4681 caatgatttg tttaaattgt tgctttggtc ttgctccaac aattttttcg acaacttggc
     4741 cacccttaaa aaagaggata ctgggaatgc tcatgatctg gtattgtccc gcaacttcgg
     4801 gggcatcatc ggtgttgagc ttcatgactt taagcttgcc ttgatactcc tgtgcgagct
     4861 catcgacgat tggggccacc atttgacagg gtccacacca gaccgcccag aaatccacca
     4921 tgaccacctc tgaagatttc agcacttccc catcccaatt cgttgcatcc gctttggcca
     4981 ctaaatcaga cactgactgc ctccttggat aaatacgaca aaatgcccag ctctgatggc
     5041 atcctaaaac cgctcttgtc ggactgtcaa ctcattcata caggcttagc tagctaaaga
     5101 gggctagccc ctatgatttg agggatctct aacactcgca gaaatgacag gaaccgaatt
     5161 caccactgcc attcatccgg tgtgccggtc caccatgaat caggattcaa ttcgcgccac
     5221 cgggtttgtt gtgcccataa ttcttccgtc tgtgtccgac tcaaccgatt ggccatcgct
     5281 gctgtcacgt caaattgttg tcgaatgcct tcatgaataa gctcttgcgc catacgaggc
     5341 atgacatttg gcggatcaat gagatcaaga atgtcccgtg cggtcatatt caaagctaac
     5401 tgctctactt tcacataatc ctcctgcttc gctaatgatc ctaaataacc atcaattgcc
     5461 tgatatacgt aggccaaata aacgtacgct tcaaccgagg gattgcggtc aatatttttt
     5521 tggcaagcct ccaccgctcg ccggtaatct ccggcactca aaaacacttt agcatgcgcg
     5581 agaggcttac tagtcaggat aggaatttgg tctgactgag cccaaccatt ggaatgcggc
     5641 aacgtaacca gaagaattac tcccacgccg aaacacacca tcagttccca tgctcttttg
     5701 ctagccgaca aagccctcat gatctcctcc ctcactaaaa tcttgcaata tgcagcattc
     5761 tcgatagatt cccaggttgt tcaccccatt ttccgctgat tcaattaccc agttttaaga
     5821 ttgtcggatt tttaatcagt aggagaatcc cgaaatctcc ctgtttccaa tgcctattaa
     5881 aaaaaaaccg tttccccttg tctatgatgt cccggaacca cgacataatg ctgtagggaa
     5941 ggccacttgt tatttataaa aatccaaaag gcctaacacc aaactcccca ctacaataca
     6001 agactcccat aaaaacgagc cattgaacaa tctaaaactt caatggcata taccttgcga
     6061 aagattttat ccaaaaagca ttacattgaa cttagctcct ttattaaaaa aacaacgcgc
     6121 aaacaattta aacccatcac cactaaaaaa gggataaaat gaaacaaaac aaaagacttt
     6181 tgccttttat cctcatcatt ggcctagaaa ccttcttttt atgcttaatc ccggtctccc
     6241 ctactcacgt aaattcggct gatatcgctt tggttctttc gtcttcttcg cctggctcat
     6301 cattgccgct agaaatgacc cagacgaagg attccaccca cagcgacgac acattagccg
     6361 ccctaacaat ctacctggaa gcccgtgggg aaagttttgc tggaaagtta ggcgttgcgg
     6421 ccgtcattcg gaaccggatg actcacaaat accacagcga tggcacggtc aagggcacag
     6481 ttcttcgtgc caaacagttt gagccatgga tcgggcggaa tccaaacgaa gtgaaatttg
     6541 atcaaacgaa tcgaaaaatg caagaaagtt tattagcctg gaggcttgtg caagacggtc
     6601 ggaaggtagt gaatggggct gtgctgtttt ataatcccaa actggtcaag accccccatt
     6661 gggctcaggt caaccgaaaa gtggcccaaa ttggcggaca cgaattcttt aataagcaca
     6721 ccatttaact agtcattgcc tgcacgcgtt tttcgtaaat tcttaggccg agtcgcactg
     6781 cgtcccccat cctcaatacg tttcaactct atgcgtaaat gtcggagtgc accggaatgc
     6841 acccgacaga cgccagattc ggtaagcccc atttggtgac caatttcctt catcgttaat
     6901 ccggtgtgat aataggactg aagtacttct tgctggcgtg ccgataatgt ctcaagggcc
     6961 gctgataatg ccgccttcat ttctgcgtcc gcacaggcca caaaggggtc cagatggtta
     7021 acatctggta aaacatcacg aagcgtacat tcatcctctc cttgcccaac cggctcgtcc
     7081 agactaatta aacgaggatc acaccctccc actccctcta tttcatcaat gggaacgccc
     7141 aatagcgcag ccaattcctg tgaagtcggt ggcgtcccgt ttttctgaag atgttcttca
     7201 acaatttgtc gcacttgatc tcgacgggat cgaacagacc gaggaaccca atccattgcc
     7261 cgaatttcat ctaacatcat gccacggatg cgatattcgg caaatgttct aaatttgata
     7321 tcccgtgttg gatcatagcg ctttaacgcg gacaacaaac ccattgcccc ggcgctcgtc
     7381 aaatcctcga catccaggga aaagggattc ttgaacgcca taactccagc catccggcga
     7441 atgatcggga gaaactccaa taaaatccga gtaacatgtt cctgatcaag ccggtcctgc
     7501 gcttctccat tttctgcttt gtcctgacta actgagccaa ggcaagacga gtcgagctct
     7561 ttgatgccaa ttgctggctc tgtgaaatgt tgcgcgtcat cgtccatatt tcggcctttc
     7621 cttgtgacca aaaaaaaagc gcccaattcc agggaatggg cgcatgaacg aacgcatgga
     7681 cctaccaaca cagttcggta accagacgaa ccctaaaaca accacgctct cagggtttct
     7741 tggctttgcg ccctaccctc acggatagtt tgcccttgtc acctgttgaa tttgttatcc
     7801 gataatcaca accttatgta agtggtgagt ttttgaaatt tccgatccac tgtctgcctg
     7861 aaagcattgg ggcctcctct acccttcaag ctctgtctct gtgcaagtgt tatcggattc
     7921 aagacagggg gagttaagaa aaaaccttgc gtgttcatga gggaaattga caaattcata
     7981 cattgcacag catcaggaac aaacactaat ggtgccagca aacaaagacg aaaatttcaa
     8041 tcacacattt ttcggagcaa tcttcaaggc aatcgcgatt ccctcatttt agcttcaaaa
     8101 aaaggttaat tccgatgacc aaaaacatgg ctgtggcaat ccaactacca atgatcggta
     8161 acaccgctcc attgcgtcct aaaacaatac ccacggaatg tgcggcccag aagaaaaatc
     8221 caattgccag agtttgccca agccctttcg ccactcccac cccacgcaca ccacttcctc
     8281 gaagactcac cgagatccct agcaatgtca tcaccagagg aacgatggag tacgcaaaac
     8341 gagcccaata atcagttaaa aaacgatgag gactatggcc atcttcaatt actctctgaa
     8401 tataatcatg caattcatac aacgacatgt gctctggaga ctgagatagc caattctgaa
     8461 aatcatcagg cgtgagagag aggcgcaaca cctgccgtgg catgccattc acttgcatgc
     8521 gttcttcttc ctgaaatgtt cgttgaatca catgctcaat cacccattca tcatcgacaa
     8581 atgttgccca ttccccttca agaattccct tgagattgaa gcgatcatcg agtgtgtact
     8641 ggtgaatacc attcaaacga tgtccatcct ctgtcaccgt atcaatacgc aaaaggtgat
     8701 ttcgtcccac cctgagccac aaaccttccg gagtgagtga gaggggctct ggtttatttt
     8761 gaatcaggac cgattggaca tattcggctt tgatattggc cagaggaatc aacacagccg
     8821 tcaaactcag acccacgaca ctcacgagcg caccaaaaac aaaaaaggga atggcaattt
     8881 gataaaaact taacccgcaa ctgcgcatcg ccgtaatttc acgagtccgg ttaagcccac
     8941 caaccgtcaa aattgaggcc atcagggccg ccagcggggc cagtaaaaag gcaatttcag
     9001 ggattcgata gacaaaaaag ctaaaaataa ccgttaagtc cgcatcatat tttaaaaatt
     9061 ttcttaattt ttcgaaaaaa tccacgacga gataaatcgt caacaccgtt acaagacaca
     9121 tcaggaagac ccggctaaat tttccaaaca cataccaaga aattttcttc atgatctaaa
     9181 tctggttgaa aaatttcttg atcattgctt tcgtgcttga aatagtaaga atcctgtcag
     9241 gcaggccagg atgatgtttg gcatccatgc gccagctaat ggagaaatca ccaaagtcgt
     9301 cacgagaaac tctcccagca catttaatag ataaaacccg atgaccacga gaaccccaat
     9361 cgcaaaccct ccggcttttc ccgaccgctt cgacacaatt ccgaccggaa cgcccaataa
     9421 acccagcaca aaggtggcga ccggaaaggc cgtgtcttta tgaaactcta ttaatcgacg
     9481 aagggcaccg gcatccgttc cacccgattc ttccaattgt tgagtgaggt cttggtaggt
     9541 tttccgtttg gttttttctt tcactgacgt aaatggtgaa gacagccaaa aatcataggt
     9601 atcaaaggcg acccgatgaa aactattggc atctttgggg atttgatgga ttgcccccct
     9661 aaacaagcga ataccaaact gctgacgttc gctctgtttc aacatttgaa aattctccgc
     9721 cacaataatc aagggttggt cgggattacg ctgatccgaa ataaatacgc ccttttggcc
     9781 agagccattc tctgagtgag gcacatacac catgagtcca gggatcggtt cattaaacac
     9841 acccgaatca acagcaagag tcaactcatc ttcgatgacg ctcagagcca aagtcttcaa
     9901 tgacactttg gcccacggct ggccccattg agaaaggtac accgttaaaa gaaataccat
     9961 tccagaaaag accgccacag gaaggttgat ccgccagaaa ctaacccccg cggctcgaat
    10021 ggcagtcacc tcattgtcaa acgataggcg attaaatgcc gaaattgagg caatcaagca
    10081 ggcaattggt aaggttagga ctaaaaagga aggaagcaaa tgcagaacaa tcgaccccaa
    10141 gcccgccaag ctaattcctc tagataccac taaatccacc agccgtagca tttccttcgt
    10201 aaaaatgatg aaacacaatc cggtgaggct ggtcaaaaat gggggaagta actcaaaaaa
    10261 aatgtaccga tcgagaagtc tgatcaacat cttggacaat acggatgaat aaaaagtgat
    10321 aaaaaatgtc ggataaccaa catggccccc tcggccatta attccaaatc tggaacaatt
    10381 accctaccgg cgaaatagta gggaatgaat ttcctcctgt catttattga cagattcttt
    10441 aaactgtgct acaccatcct cgtttttgta ttatggttaa agaagtgaaa aggatattac
    10501 gccgtgaagc cgagagagga atgaactcct ttttcggctt tttttttagg aacgcacaaa
    10561 ttttataatc tatatccaca agtgctctaa tggggagggc catcatgaga agtaagggaa
    10621 cggtaaaatg gtttaatgat cgaaaaggtt ttggattcat tcaagttgaa ggtggacagg
    10681 acgtgtttgt ccattattcc gctcttcaag aagacggatt taaatcccta aaggaggggg
    10741 aatccgtcga atttgacttg gtagaaggag caaagggtcc gcaggctgcc aacgtcacta
    10801 aatcaggaat tggccaacct tcgtaagcac tgttgaatat ctgtcgcctt cttacctact
    10861 tttgagtggt gaagggggcg aggtttttgg gttccctatg gatcaacttt tcttcaaaaa
    10921 taataaggta agagtattcc ttgatctgtc ataacttaca attgacttgt tatctaagaa
    10981 ggttaggagg tcatgggcct gctaagcctt ccttgacttc gctatttttc tcctggtaga
    11041 cttttctcgt aagtcgttgt atttattgca aaatttcaaa ttatacacaa ttgactcatc
    11101 aatttcagag gaacagcatg acaaaacttc tcgatgcctt attttttgaa aacccgctat
    11161 ctcgaaacag gttgttgacc gtagaaagtg gggaggaaga ctggggagca ggacaaaacc
    11221 aggatgccaa tattcctcag gcaccagaca acttacgtgc tatccctgat catacgcgca
    11281 ttaccctcac atgggatcct gtccctgaag ccctgtatta caacgtttat ttcatgacca
    11341 ccaaaggcgt tcaaattaag ccaaatgaac tgaccagacc agtcgcaggg catgaggatt
    11401 ttatcccaaa agttgggatt acaaaagaaa aagccaattg cattgaagga gcgatatctc
    11461 cttatgaaca taacgactta gccaataact tctgttacca ctatgcaatc tctgtggtca
    11521 cagaagaggg tgaaagtcta ttgtctgagg aagtgatgtc gattccctcc ccctatctct
    11581 ccgtaatgca aatcggatgt gaaggcgtcg acgatggaga attcaaatca ccaaccggca
    11641 ttgctattga taaagacgga aatatctatg tcggcgatac agacaatcat tcaattcaga
    11701 agtttgaccg atcgggaaaa tttcttgccc gttgggggga cgaacccgat tcaagcgaag
    11761 gaaaatttta ttacccacgt ggcatcgcca cctcaccaga aggagaggtg tatgtggccg
    11821 acagtgggaa caatcgcatc caaaaatttg attcagaagg taatattatc aacgcatggg
    11881 ggaaatttgg ttttgcctgg cgaggggcgg aagccgggaa atttgatgtc ccttggggtg
    11941 ttaccaccga ttcccaaggc aacctgtttg tctctgatac cagcaatgca agaattcaaa
    12001 aattcctgcc aaacggatcc cctgtgctca agtggggccg tgacggggga tatgacgggg
    12061 cgttcttttt tcctcgcgga ctcgcagttg actttgtagg gaatatttat attgctgatg
    12121 aggggaacaa tcggattcaa aagtttgatg cacgcggtaa ctttctcctg aagtggggaa
    12181 aggaagggaa tgcccctggc caattcaaag ccccatgggg agtcgcctgc gacccgttgg
    12241 gatatgtgta cattgtcgac agcggaaatc accgtgtcca aaaatttgat tctagcggaa
    12301 cctatctaac ttcatgggga aatcgtggat taaccgaagg acagctgaat ttcccgtcag
    12361 ggatcgcagt agacaaagag ggatatgtat acgtaataga cagcggcaac catcgtttaa
    12421 ccaagtttgc cccaacagaa gaagaactca atcgtggcaa acaagaaaaa cgggtttcca
    12481 gcgatcttac cgcccccatc aaccttgccg ttaagccggg ggatacggaa aacatcctta
    12541 gttggctcga agttcccaat gccgtcagtt acaacctcta ttttagtacg gaacccaatg
    12601 tctctacaga aacttcaaca aagattgaag gcgtgaccac cccctatatt catagtggtc
    12661 tcacgaatga cacgccatat ttttatgcca tgacttcggt cactgaagaa ggagcagaaa
    12721 gccagctttc tgaggaacag accgctattc cggttctgat cgacatttcg gcacctcaaa
    12781 atcctgacat cgtcataaac catggcgctt acatgaccaa tagtcccgac atggtcgcca
    12841 ccatctcagc taaagacgta gatagcgggg tggctgccta tttcatctct gaaaacccca
    12901 tgactccaag cgggaccatg cctgggt
//
LOCUS       DAORAQ010000068        12822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_813_length_12822_cov_63.701966, whole genome shotgun sequence.
ACCESSION   DAORAQ010000068 DAORAQ010000000
VERSION     DAORAQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12822
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_813_length_12822_cov_63.701966"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PKM72_07915"
     CDS             complement(<1..1299)
                     /locus_tag="PKM72_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNP60748.1"
                     /translation="MLFIRFLIFFLIAMLGMVPPVLSEVDIDKLKKGVVKITAQFAKR
                     QKVGTGFVAGQGKKHLFIVTASHVIEGEAEVPQSITVTFFSHQEEPLVAEVINKEGGD
                     PRGLALLKVGGDLPDDVEILEWDTQSQLHGGEDVRVIGFPQVGGNPWAVTRGTLSGFD
                     GPILKFSGAVEEGNSGGPLLYRGKVIGVIVEIIRQFGNAKPSQIAQFTVDNWPGFSRQ
                     VRIEPKNISPGSTTDTGELTGKKLAALVVTTVPKDAEVFVDDEFVGNTTQGPVVVGQL
                     SPDEYEVAVKKKGYRPWFMNVELLPGERRALNATLQEGAVVDVTGIWKNPDEPTVVYV
                     LQQIGDRVVMREVTTSVLGTMVTAEGEGQLQGNQLSIFYRTVLGTMGQSATTISDDGR
                     TLTGTFQDFSNMIPISLSLVRSEDSPASFGLPGNDAWNAIQ"
     gene            complement(1566..3077)
                     /locus_tag="PKM72_07920"
     CDS             complement(1566..3077)
                     /locus_tag="PKM72_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNP60749.1"
                     /translation="MKDVFISYASEDRPVAQQLAEGLEQSGISVWWDRQIQVGSEWDK
                     TIEDALADAKCVVVLWTGHAKDSRWVRAEAREALIKEKVVPVMLEANAIPLAFTGIQA
                     LCFLGWNGSSRSKEFEILLGVIKAKLEGKSVEFPEASSTKASVVGKLVALLGIRTMVG
                     GIFALLLIGSSFVRVAPVISVHVETARIEFTVAPGLDNKRLTDAITFDSLTVENVGKL
                     SVTPDRLLVADPADYDMTSDSYPPKAWLDLPINGRTVQFAADRPSISSEITIETTEDK
                     SAAAGQLDGIVLTDEALVSMEVSNDNALTWSIRAKQGRQRVVMSRIHAVQLLETGMKA
                     SPGLSIPFPQDQELTYQIIFEQKPGTIELFGQDQAFVLVLKEKETTAKKPISGSVLPI
                     QSVDFSWQDPGTGERKPPEDFSGTVEYVSPHGMPSVPVGMNAFLTLDDLDHFEITSIS
                     IAPVSHTFIVDLKGKAGYVKTGTAGNPQDLRPTLFDRIRYSPLFEPVKKIIGF"
     gene            complement(3097..3873)
                     /locus_tag="PKM72_07925"
     CDS             complement(3097..3873)
                     /locus_tag="PKM72_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60750.1"
                     /translation="MIKRYAYGLFLTLVFLAAVPSLLWAQPDLEYQIRRQKQSGSWKE
                     GVKPKPVSGLNIELISVLADYQETVSSDNFPNGVKLQFYLDDEHAVYLTVRELDYRTY
                     YWLDKVEPARIWKKGFQNVFAWPTDPVLRQLTPKLDLYELGALVRLDAETTSSLERVA
                     PAVLYHKDAPSEIDGYFFTLKTGEDARLMATITREATGQEVETQMFRRKRAGRPFTIH
                     WDAKDAEAGFYKLDISGFSLSTNQTIFKEIHFYHQPIVTP"
     gene            complement(4430..9988)
                     /locus_tag="PKM72_07930"
     CDS             complement(4430..9988)
                     /locus_tag="PKM72_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNP60751.1"
                     /translation="MKRLRVHGQREPLDLKSATPDFPGNLKDLVTLKHSARVQGARAG
                     QPPLEIDDLSSDDLIQLELDQGIKLWVRADDLERDFGLTPSRGSTGDVVELPLALPLG
                     TPSRGIVGNWVIKGFKVFGLDPVGATTDLIKDKVEGVMKPGPGLYRWEGKESSDYTRI
                     KSLAKEKTDTPWLVFIHGTASSTEGSFGGLWEGGANARMVQLLGHYPKRVLAFQHRTL
                     SQNPIQNALDLAGYFPEGARLHLVSHSRGGLVGELLCRSIMEGRFSFDQDDFQVFAQP
                     DRKDDLKQLEQLGHLLQQKQLIIERFVRVGCPARGTTLASGRLDRYLSIILNVVEAIP
                     GLKGNPVLEGLSAFLLGVVKNRTKPEELPGLEAQMPTSPLVRILNRPGIRSMADLHIL
                     GGDVAGEGVLGRLKTFVTDLFYREDHDLVVNTPAMFGGTERTGEVQYWIDTGKEVDHF
                     HYFRNPDTANRLMQALTKDAPHSLFHRLEREPSEVTEDDYRKRAVGSPQPVVYVLPGT
                     MGSQLKVGNDRVWLDKLDLAFGGLKKLKYTAKNVEADQPIGSGYKDLIKYLANSHTVK
                     PFAYDWRKSLVELADRFRKDLEETVKAQEAVGQPVRIIAHSMGGLVVRVMMATEEGKK
                     VWDRMCRHPGARFIMLGTPNEGSHAITGMLMGRDPLVRMLDLLDITNSQTTLLGIISR
                     FDGVLQLLPHTGTLDVYQVETWRSLLEHDRDRVRGLFGAKVATSKTAGIEWPVPDAAQ
                     LADAFKVQRLLQDSPIDSQRMIYVAGRADATPCDVSIDLSDSAERRIRIDATPFGDGR
                     VPWDTGIPEALKHQTYYMDSEHGDLANAPEAFDALLDLLNAGVTTKLSQVPLVRRGVS
                     VVPFELPEVRLEMYPNEEDLIASALGSARIKKKRQPTRKISVTMVHGNLSRASSPVAV
                     GHYEGDTIVSAEAYLDRQLNGRLRERQCLGLYPDKLNTSAVVLTDGDCDQERTHPGAI
                     VVGLGMVGDLTPGGLTSTMADAVVNYALKCIEVERKRRSATAVDGSCGPRLNIPLTML
                     LIGTGAGGLSLIDSLQSLLRGILQANRRLDKLERTETPESEGQTADDDRLTGSIETVE
                     ILELYQDLAIQATKSLLELGRSRDFREHFNIHEVMVEGVEGRRRASFDEDAGWWQRLR
                     IAIQEDGSLKFEALTNRARAETYLQPAQRKLIERFLERAMATTQNDLELSSTLFELII
                     PNRMKEYAPDRRSMVLVLDEKSAAYPWELLHNRFDPNSRPLAVEAGMVRQMAVPQFRE
                     KVLHGTALNALVIGDPTSHEPLGKFSPLPGAAREAREVAQLIRHQEYREVVELVEGDA
                     TPDAILTALYQQPYRIVHCAAHGVFEFPIEEELAKLGIPGSQRDPGTKPKTVTGMVLG
                     EGLFLTPAEINQMRYVPELVFLNCCHLGQTKGSAEQSNIPFHQLASNLGTQLIRMGVR
                     AVVAAGWAVDDLAAKTFAKKFYEEMFQNRTFGDAVRLAREEIFLRQGGSNTWGAYQCY
                     GDPDFSLHIGAKSIGRERRMVAPVELREELYNLVQEAKTAEPKDEARLRRRLQELTAG
                     VGQGWTDSAAMCAALGLAYGELGLFAEAVRFYDRGRTLQPADATVESLEQLANLKVRW
                     ALDRVERQGDPTAQTLHPIEKDFPIQDLFDDAENILAGLLTIQPTQERYALKGKLYKG
                     KAMLLTTKADQRKALLEMKRCYDEAYTIGKAVKRNDAYYPLENRLAAEIVLSWDQPKG
                     RQPQRGKKKGPDPITEGLADLATYAKNFKENRQSFWDISLPPDQKLLEALYAQRMTGK
                     DQKEIGNGYLEAKRRGGSAREMDSVIKHIRFFELMVATQAPPSIRQQLSAGLKTLRVN
                     IVSDDGGKGV"
     gene            complement(10688..12169)
                     /locus_tag="PKM72_07935"
     CDS             complement(10688..12169)
                     /locus_tag="PKM72_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNP60752.1"
                     /translation="MIAKKHVPMMLVILCLPGCLVGPDYQQPDLNVPEDWNRMTLEGS
                     SSLLPITVGQVPEAEWWRMFANDELNGLIEQALDRNHDLRQAAFRVLEARAIAYGSGA
                     GLYPNVSLNGSYSRIRRSESILVGPTGGAPEGFAPPGANFDIWRADIDLRWELDLWGR
                     IRRGKEAFTAEALGSEMNRRGLLLSLVGEVGDAYFRLRELDEQVEIAERNLALQQDSL
                     SIIRSRAQAGLVSDLDVKREETLVAQTASQIPEFRRQRAVQLHQLEVLTGANPGTLVL
                     SSKPLRSVMAQPTIPVGLPSDLLQRRPDILEKEETLKAANARIGEARAYFFPSVAITG
                     NGGFLTSEFDQWFKWGSRNMAIGPSVTLPIFEGYTNLARLEVAENRYQQMLEDYQQTI
                     LNAFREVADVLVALQTRKEQLVSQHQHVAAARESLELADIRYRKGLVSYIDVIDAQRI
                     VLDAELGVVQTERARLTAMVDLFKAVGGGWAQERLAQVIQEKP"
     gene            complement(12180..>12822)
                     /locus_tag="PKM72_07940"
     CDS             complement(12180..>12822)
                     /locus_tag="PKM72_07940"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP60753.1"
                     /translation="KRLQSVTDRRAVSEDDVKRKWHETEGAKRALVRARADLHLARTE
                     RDEVQALLNRLTVRAPRGGTILQVNVRAGEYATVNPIEPLILLGNTETLQVRADIDEV
                     NAPLVVPGSPAVAYLKGFTDKSISLTFDRIEPYIVPKRSLTGDNRERVDTRVLQVIYG
                     FERPSFPVYVGQQVDVYIERSAVQVSGQDQSQWGKGAQQSDVSRPLSKAGETQ"
BASE COUNT         3066 a         3675 c         3020 g         3061 t
ORIGIN      
        1 ctgaatggcg ttccacgcat cattgcctgg taaaccaaag gaggcgggag aatcttccga
       61 tcgaaccaac gagagactaa taggaatcat attggaaaaa tcctggaagg tcccggttaa
      121 ggttctccca tcatcggata tcgtggttgc agattggccc atcgttccca acacagtccg
      181 atagaaaatg gataattggt tgccttgcaa ttgtccttct ccctcggcgg tcaccatggt
      241 tcccaacaca ctggtagtca cctctctcat gaccacccga tctccaattt gttgcaggac
      301 atagaccacg gtcggttcat ccgggttttt ccagatacct gtcacatcaa ccacggctcc
      361 ttcctggaga gtcgcgttca acgcccgacg ttcgccggga agaagttcga cattcataaa
      421 ccaaggtcga tagcctttct ttttgaccgc cacctcgtat tcatcgggag ataattgacc
      481 cacgacgact ggtccctgtg tggtgtttcc cacaaattca tcgtcaacaa acacctccgc
      541 atccttagga acggtggtga caaccaacgc ggccaatttc tttccagtta attcaccggt
      601 atctgtggtg ctccccggtg aaatattttt gggttcaatc ctgacttgac ggctaaaccc
      661 cggccaatta tcaacggtga attgagcaat ttgagacggc ttggcattac caaactgccg
      721 gataatttcc acaatgacac cgatgacttt tccccggtaa agcaatggtc ctccagagtt
      781 cccttcttca accgcaccag aaaacttgag aatgggtccg tcaaatccag acagagtccc
      841 tcgggttacc gcccatggat tgccaccaac ctgtggaaac ccgatcaccc gcacgtcttc
      901 acccccatgc aattgactct gggtatccca ctctaaaatc tccacgtcat cggggagatc
      961 tccgcccact ttcaacaggg ccaaccctct gggatccccg ccttctttat tgatcacctc
     1021 agcgaccaac ggttcttctt gatgagaaaa aaaagtaacg gtgattgact gagggacttc
     1081 agcctctccc tcaatcacat gtgaggcggt cacaataaac agatgttttt tcccctgtcc
     1141 agccacaaag ccggttccga ccttttgcct cttggcgaat tgagccgtga tcttgaccac
     1201 accctttttc agcttgtcga tatccacctc ggacaagacc ggtgggacca tacccaacat
     1261 ggcaataaga aaaaaaatca gaaaacggat gaatagcatg ggcaagtcct atactctgtc
     1321 ataaggttca catacccccg taatcttgtc acaacaatcc taagaaacca agaccattca
     1381 cccagaactc gtgcaatgga atgaagaaag ttcggcagaa gtcatcatct gtttttcctc
     1441 ccaaaatttt cctgaatctg ccctttcata taaacgtgat gatgatttca tctgtgatga
     1501 aacttaatgt taatcaaccc ggacaatctt ttcttcgtac actttttttc atccaggaat
     1561 gacacctaaa atccgattat tttcttgact ggttcaaata aagggctata tcgaattcga
     1621 tcgaagaggg tggggcgaag gtcctgtgga ttaccggctg tccccgtttt gacatagcca
     1681 gccttcccct tcagatccac aatgaatgta tggctgaccg gggcaatact gatggacgtg
     1741 atttcaaaat ggtccaggtc atccagggtc agaaatgcat tcattccaac cggcaccgaa
     1801 ggcataccgt ggggactcac atactctacg gttccagaaa aatcctctgg cggtttgcgt
     1861 tcaccggtac caggatcctg ccaggaaaag tctacagatt gaatcggcaa cactgaaccg
     1921 gaaatgggtt tcttggctgt ggtttctttt tctttcaaca ccaggacaaa ggcctggtcc
     1981 tgtccaaaca gttcgatcgt tcccggcttc tgttcaaaaa tgatttgata ggtcagctcc
     2041 tgatcttggg gaaagggtat agagagtccc ggagatgcct tcattcctgt ctcaagaagt
     2101 tgaacggcat gaatcctgga catcaccact cgctgtcgcc cttgcttcgc tcggatcgac
     2161 caggtgaggg cattgtcatt ggaaacctcc atactcacca gggcctcgtc ggtaagcacg
     2221 atcccatcga gttgtcccgc agccgcagac ttatcctccg tagtttcgat cgtgatttcc
     2281 gaggagatgc tgggcctgtc cgctgcaaat tgtacggtcc gcccattgat cggcaaatcc
     2341 agccaggctt tcggtggata actatccgac gtcatatcat aatcagcggg atcagcgacg
     2401 agcagcctgt ctggagtgac ggaaagcttc ccgacgtttt caaccgtcag ggagtcaaag
     2461 gttatggcat cggtcagtcg cttattatcc aaaccgggag ccaccgtaaa ttcaatacga
     2521 gccgtctcaa catggacgga aatcaccggg gccactcgca caaatgaact gccgataagc
     2581 agaagggcaa aaataccgcc caccatcgtc ctgatcccca aaagggccac cagcttcccc
     2641 accacggacg cttttgtgga agaagcctcc gggaattcta cggacttccc ctctaatttt
     2701 gctttgatga cgccgagaag aatctcaaat tctttggacc tggacgaccc attccatcca
     2761 agaaaacaca aggcctgaat acctgtaaac gctaatggga tagcattggc ctccaacatg
     2821 accggtacga ccttttcttt aatcaaggct tcccgagcct cggcccgcac ccatcgggaa
     2881 tctttggcgt gccccgtcca taacaccacc acacatttcg cgtcggccaa tgcgtcttca
     2941 atggttttat cccactcgct gcccacttga atctggcgat cccaccaaac cgagatcccc
     3001 gactgctcca agccctccgc caattgttgg gccacaggtc gatcctcact ggcgtaactg
     3061 atgaacacgt ctttcattcc tgaaggggta ctctcgttat ggggttacaa tgggctgatg
     3121 ataaaaatga atttctttga aaatggtttg attcgtcgaa agcgaaaacc cgctgatgtc
     3181 cagtttatag aatccggcct ccgcatcttt ggcatcccaa tgaatggtaa aggggcgccc
     3241 ggcacgtttt cttctgaaca tttgcgtctc cacttcttgt cctgtggctt cccgggtgat
     3301 ggtcgccatc aatcgcgcat cttcaccggt tttcaaggta aagaaatagc catcaatttc
     3361 agatggtgca tccttgtggt aaagcaccgc aggcgccact cgctctaagc tggaggtcgt
     3421 ctcggcatcc aggcgaacca gggctcccaa ttcatagaga tcaagtttgg gagttagttg
     3481 tctcaggaca ggatcggtcg gccaggcaaa gacattctga aatccctttt tccagatccg
     3541 ggcaggttcc accttatcca accaatagta ggttcgataa tccagttcgc gaacggtcag
     3601 atacaccgca tgctcatcat ctagataaaa ttgaagcttc actccatttg gaaagttgtc
     3661 agaggaaacc gtttcctgat agtcggccaa aactgaaatc aattcgatat tcaatcccga
     3721 gaccggcttc ggtttgacgc cttccttcca actgcccgat tgcttttgac gccttatttg
     3781 atactctaaa tccggttgag cccagagtaa cgatgggacc gctgcgagga aaaccagagt
     3841 caggaataga ccatacgcat atcgttttat cacaacgttc tgctccgcct cgccaatggg
     3901 gtaaaatctc ataatctgaa gacgttcgag tgagaaaccc gaatatcgat gtcgagaagt
     3961 ctaacgcgga cgagaaaacg aatctagagg aacaaaggat tcgggcaacg gcctaatgtt
     4021 ctacacacat cgccattcag gtagttttga aagagccacg attcaaagag agattccggg
     4081 gaacaacttg cgggaatgcc gggggcagct ttagtgatat tagacatcca gatcattggt
     4141 gaacccctca gataacatgg aaaaaaaatt cagggaatta tttcgatcaa gaactggccg
     4201 gaaggggtgt aattgataga gagattatga tcggaaaaaa atcggaaggc gaaggtaggc
     4261 gtgtttacct cagcaagatc gtcttgtcac tatgtactcc cacccagtcc tcgctaactc
     4321 aatcgacgct ctcttgctac atgcgaggta ccttgtgagc cctctttctc ccaatccagg
     4381 cctctgaaaa ttcggcagat gaacatcttc ccggccattg cttcttatct cacaccccct
     4441 tacctccgtc atcggataca atattcacac gtaatgtttt caatccggcg ctaagctgtt
     4501 gacgaatgct gggaggtgcc tgcgtggcaa ccattaattc aaagaacctg atgtgtttga
     4561 tgacggaatc catttctctg gccgaccctc cccgtcgttt ggcttcaaga tatccattac
     4621 caatttcttt ctgatccttt cctgtcatac gctgtgcata taaggcctca agcagttttt
     4681 gatccggggg cagacttata tcccagaagg attgtctatt ttctttaaaa ttcttggcat
     4741 atgtggctaa atcagccagt ccctctgtga tcggatcagg tcctttcttt ttccctcgtt
     4801 gtggttgccg tcctttcggc tgatcccacg agagcacaat ttcagccgcg agtcggtttt
     4861 ccaagggata atacgcatcg tttcgcttta ccgctttgcc aatagtatag gcttcatcat
     4921 aacaacgctt catctcgagt agggctttcc gttgatccgc cttagtggtg agcaacatgg
     4981 ctttcccttt atagagcttc cctttgaggg cataccgttc ttgggtaggt tgaatcgtaa
     5041 gaaggcctgc gagaatattt tccgcatcat caaacaggtc ctggatagga aaatcttttt
     5101 ctatggggtg gagcgtttgt gctgtgggat ctccctgtct ttcgacgcga tcgagtgccc
     5161 atcggacttt caaattggct aactgttcca gcgactccac cgtcgcatcg gccggttgca
     5221 gggttcttcc tcgatcatag aatcggaccg cctccgcaaa gaggcccaat tccccataag
     5281 ccaaccccag ggcagcacac atagccgcgg aatccgtcca cccttgacca acaccggcag
     5341 tcaattcctg caatcggcgt ctcaaccggg cttcatcttt cggctcggct gttttggctt
     5401 cttgaaccag attatacaat tcctctcgca attcgacggg agccaccatc cggcgctccc
     5461 tacctatgct tttcgctccg atgtgtaacg aaaaatccgg atccccataa cattgatacg
     5521 caccccacgt attggaaccc ccctggcgaa gaaaaatctc ctcacgtgcc agccgcaccg
     5581 catcgccgaa tgttcgattt tgaaacatct cttcatagaa tttctttgca aaggttttag
     5641 cggcaaggtc atccaccgcc caaccggctg ccaccaccgc ccgcacaccc atgcgaatca
     5701 gttgcgtccc cagattggag gcgagttgat gaaacggaat gtttgattgt tcagcactgc
     5761 ctttggtttg tcccagatgg cagcaattca aaaacaccaa ttcgggcaca taccgcattt
     5821 gattgatttc ggccggtgtc aaaaacagac cttccccgag gaccatcccc gtgaccgtct
     5881 tgggtttcgt accagggtcc ctttgggagc ctggaattcc gagcttggcg agctcttctt
     5941 ctattggaaa ttcaaaaacg ccatgcgccg cgcaatgcac aatacgatag ggttgttgat
     6001 acagcgccgt cagaatcgca tccggtgtcg cgtctccctc cacgagttcc accacctcac
     6061 ggtactcctg atgccgaatg agttgcgcca cttcccgagc ttccctcgcg gcacccggca
     6121 agggagaaaa tttccctaag ggttcgtgac tggtgggatc gccaatgacc aacgcattga
     6181 gggctgtccc atggaggacc ttttcccgga attgcggaac cgccatttgc cggaccatcc
     6241 cggcctccac agccagagga cgcgagttgg gatcaaaccg attgtgcaat aattcccaag
     6301 gataagcggc agacttttcg tccagtacca gcaccatact tcgacggtcc ggagcatatt
     6361 ccttcatccg attcggaatg atcagttcaa acaaggtgct ggataattcc agatcattct
     6421 gcgtggtggc catagcccgt tccaaaaacc gttcaataag ttttcgttgt gccggctgca
     6481 aataggtttc ggcccgcgca cgattggtga gggcttcaaa tttcaaggag ccgtcctcct
     6541 ggatggcaat gcgcaatcgt tgccaccaac ccgcatcttc atcaaatgag gcacggcgac
     6601 gcccctcaac tccctcgacc attacttcat ggatattgaa atgttcacga aaatctcgtg
     6661 accgtccaag ttccagtagc gattttgtgg cttgaatggc cagatcttga tacaactcga
     6721 gtatctcgac tgtctcgatg gagcccgtga ggcggtcatc atcggcggtt tgcccttcag
     6781 attcaggcgt ctccgtccgt tccagcttgt ccaaacgccg attcgcctga agaattccgc
     6841 gaagaaggga ttggagagaa tcaatcaggg acagtccacc cgcacccgtc ccgatcagca
     6901 acatggtcag agggatgttc agtcgtggac cacacgaacc atccaccgcc gtggcactgc
     6961 gacgtttccg ctcaacctcg atacatttga gcgcatagtt gacgaccgca tcggccatcg
     7021 tgctcgttaa ccctccgggg gttaaatccc caaccatgcc caaccccacg acgatggccc
     7081 ctggatgcgt tctttcctga tcacaatccc catcggttag caccacggca gacgtattga
     7141 gtttatcagg atagaggccc aggcactgtc gttcacgcaa ccgaccattg agttggcggt
     7201 ccagataggc ctcggcactg acaatcgtgt ctccttcata atgtcccacc gccacaggag
     7261 acgaggcgcg ggacaaattg ccatggacca tggtcacgct aattttccgg gtaggctggc
     7321 gtttcttttt aatgcgagcc gagcccaggg cggacgcaat gagatcctcc tcgttgggat
     7381 acatttcgag ccgaacctcg gggagctcaa aggggacaac ggacaccccc cgacgaacga
     7441 gaggcacttg ggacaacttc gtggtgaccc cggcattcaa taaatcaagc agggcatcaa
     7501 aggcttccgg agcattggcc aggtcaccat gctcactgtc catataatac gtctggtgct
     7561 taagtgcttc aggaatgccg gtatcccagg gaacccgacc atccccaaag ggggtcgcat
     7621 caatccgtat tcgccgttcg gctgagtccg agagatcaat ggagacatca cacggtgtcg
     7681 catccgcacg ccccgcaaca tagatcatcc gttgagagtc aatcgggctg tcctgaagaa
     7741 gtcgctgaac cttgaaggca tcagccagtt gcgcggcatc aggcaccggc cactcaatac
     7801 cagcagtttt tgaggtcgcc actttcgccc caaatagccc tcgcactcga tcccggtcat
     7861 gctccaggag ggaccgccag gtttccactt gatacacatc aagagtcccg gtatgcggga
     7921 gaagctgcaa cactccatca aatctggaaa taattcccaa aagagtcgtt tgggaattcg
     7981 taatatccag aaggtccaac attcgcacca gtggatcacg ccccatgagc ataccggtga
     8041 tggcatgcga cccctcattg ggagtgccca gcatgatgaa ccgggcaccg ggatgccggc
     8101 acatgcgatc ccacaccttt ttcccctctt ccgtcgccat catgacccgc accaccaacc
     8161 cgcccatgga gtgcgcgatg atccgcaccg gttgtcccac agcttcctgt gccttgaccg
     8221 tttcctctaa atcctttcga aaccggtcag caagttcgac cagcgatttt cgccaatcat
     8281 aggcaaaggg tttaaccgta tgtgaattgg ccaaatactt gatgaggtcc ttatagccac
     8341 tcccgatcgg ttgatcagcc tccacattct tcgcggtata cttcagtttt ttcaggccgc
     8401 caaaagctaa atccaactta tctaaccaga cacgatcgtt tcccaccttg agttgactgc
     8461 ccatggtgcc gggcaggaca tagacaacag gctgtgggga tcccacagca cgtttgcggt
     8521 aatcatcttc ggtcacctcg gaaggttcac gctccagtcg gtgaaataga gaatgaggcg
     8581 catccttggt gagagcttgc atcagccgat ttgccgtgtc cggattgcga aaataatgga
     8641 aatgatccac ctcctttccg gtgtcgatcc aatactgcac ctcccctgtc cgctctgtcc
     8701 cgccgaacat cgccggggta ttcaccacca gatcatgatc ttcacgatag aataaatccg
     8761 tcacgaaggt tttgagccgc cccagcaccc cttctccggc cacatccccg cctaaaatat
     8821 gcagatccgc cattgaccgg attcccgggc gattgaggat gcgaaccaac ggggaggtcg
     8881 gcatctgcgc ttccaacccc ggcaactctt ccggtttggt ccggtttttc accacgccca
     8941 gcaggaatgc ggagagacct tccagcaccg gattgccctt cagccccggg atggcctcaa
     9001 ccacgttcag gataatcgac agataccgat ccagccggcc actggccaac gtagttccgc
     9061 gagctggaca cccaacacga acaaaccgtt caatgatcaa ttgcttttgt tggagcagat
     9121 gaccgagttg ctccaactgc ttcagatcgt cctttcgatc cggttgcgca aagacctgga
     9181 aatcatcctg atcaaaggaa aatcggcctt ccatgatgct gcgacagagc aattccccca
     9241 cgagtccgcc acgggaatgc gagaccaggt gcagacgagc cccttcggga aaatatccgg
     9301 caaggtccaa cgcgttttga atgggattct gagaaagcgt tcgatgttga aaggccagca
     9361 cacgtttggg gtaatggcca agaagttgca ccatgcgggc attggcgccc ccctcccaga
     9421 gtccgccgaa actgccttcg gtagaagagg cggttccatg aatgaagacc agccacggtg
     9481 tatctgtctt ttcctttgcc agggatttga tccgtgtgta atcagacgat tcttttcctt
     9541 cccaccgata gagtccagga ccaggtttca tcacaccttc gaccttgtcc ttaatcaagt
     9601 ctgtggtcgc accgacaggg tccaggccaa acactttgaa ccctttaatc acccaattgc
     9661 cgacgatgcc ccgactggga gtccctagag gcaacgcaag gggaagctcg accacgtcac
     9721 cggtagaccc tcggctggga gtcaatccaa aatccctttc cagatcatcc gcacgcaccc
     9781 acaatttaat cccctgatcc aattccagtt ggatgagatc gtctgaactc agatcatcaa
     9841 tttccagagg gggttggccc gcgcgagccc cttggaccct ggcactgtgt ttaagagtga
     9901 ccaggtcctt caaattgcca gggaaatcgg gagttgcgga tttcaggtcg aggggctcac
     9961 gttgcccatg aacacgaaga cgtttcattg gttattcctc atacagtact ggtgatatac
    10021 ccaccgattc tccccggcgg aacaaacgct tcctaaaata tatcgacggt gactactgtg
    10081 tgtttcattc aattgccagt caaaagaaaa cggaatccca ttacagcaaa tccaccgaat
    10141 aattcctacc ctttgctatg gcatctcttc caccgaattt gtttcttcgt aggggcgttc
    10201 ccgtgaaacg tcgtcttcac cgcacgtcgg gtggccatcc acgacacatc ttctgtagat
    10261 ttgggagttg atgtcgccgg tcgtctggcc attcatgctg cacatcccac cggggcgtct
    10321 ctacatcgga cagggtggtc cgagatcaat tgagaagggt tcggtgccct ctctcgtcac
    10381 aacgtggggg tgacccatcg acttctattg agagagagac attggtgtca tgattaattg
    10441 gaactgattc cggccaatga cttgtcggaa tgacgacaat tgagaggggg aactttggca
    10501 ggcgatgcac tctacctttt tcgtaggggt ccctaccatg ccggctccac agtatcaggt
    10561 aaagtcatta atgattataa gcaacaaaaa tggttatttc tttgtcgcga cgttcccatg
    10621 gaacgtcctt ttcaatgcac gtcattcgac aatcaaggat agacgtatcg cccgatgtct
    10681 ctaccggtca gggcttctct tggatgactt gagccaggcg ttcctgcgcc caaccgccgc
    10741 ctacggcttt aaacaaatcc accatagccg tgagacgtgc ccgctcggtt tgaaccactc
    10801 ccaattctgc atcgagcacg atgcgctgag catcgatgac gtcgatatag ctgaccaatc
    10861 ccttacgata ccggatgtcc gccaactcca gcgattcccg ggcggctgcc acatgttgat
    10921 gttgactgac cagttgttct ttgcgcgttt gcagggcaac cagcacatca gccacctccc
    10981 tgaacgcatt cagaatggtt tgctggtagt cctccaacat ttgctgatac cggttttccg
    11041 ccacctctaa tctggccaaa ttggtatagc cttcaaagat cggaagggta accgagggac
    11101 cgatcgccat gttgcggctg ccccatttga accattgatc gaattcgctg gtgagaaatc
    11161 ctccgtttcc cgtgatggcc acgctgggga aaaagtaggc gcgggcttct ccaattcgag
    11221 cattggcggc ttttaaggtt tcctcctttt ccaaaatatc cgggcgccgt tgcaagagat
    11281 ctgaaggcag accaacaggg atcgttggtt gtgccatcac ggaccgaagg ggtttggagg
    11341 acaacaccag cgtcccagga ttggccccgg tcaacacttc gagttgatgc agttgcacag
    11401 cccgttgacg ccgaaactcg ggaatttgcg aggccgtttg agccacaagg gtttcctctc
    11461 gtttcacatc caggtccgat accagaccgg cctgcgcacg gctccggatg atcgacagtg
    11521 agtcttgttg aagggccagg ttccgttcgg caatttccac ttgttcatcg agttcacgaa
    11581 gccgaaaata ggcatctccc acctcaccca ccaggctcaa taacaaaccg cggcgattca
    11641 tttcgctccc caacgcttct gcggtaaagg cctcctttcc gcggcgaatg cgaccccata
    11701 aatccagttc ccatcgaaga tcgatgtccg cacgccaaat atcaaaattg gcacccggtg
    11761 gcgcgaaccc ttcaggtgcc cctcctgtgg gaccgaccaa aatgctttcc gatcgacgaa
    11821 tccggctata agatccattc aacgacacat tgggataaag cccggctcct gacccatagg
    11881 caatcgcacg agcctccagc acccgaaacg cagcttgtcg cagatcgtgg ttccgatcca
    11941 aagcctgttc aatcaatcca ttcaattcat cgttggcgaa cattcgccac cactcggcct
    12001 ccggcacttg gccgaccgtg atgggaagca gagaagatga gccttccagg gtcattctgt
    12061 tccagtcttc cgggacattg aggtccggct gctgatagtc cggccccacc agacatcccg
    12121 gcagacaaag aatcacaagc atcatgggta catgtttctt ggcgatcatg cgtttcccct
    12181 tattgagtct cacctgcctt cgacaacgga cgggagacat cagattgctg agcccctttc
    12241 ccccattggc tttggtcctg tcctgagacc tgaacagcag agcgttcaat atagacatca
    12301 acttgttggc caacatagac cgggaacgag ggcctttcaa atccataaat tacctggagc
    12361 accctggtgt cgacccgctc gcgattgtcg ccagtgaggg aacgtttggg cacaatgtac
    12421 ggctcaatcc gatcaaaggt gagagaaatg gacttatccg taaacccttt gagataggcc
    12481 acagccgggc ttcccggcac caccaacggc gcgttgacct catcgatatc ggcccgcacc
    12541 tgtagggtct ccgtatttcc caataagatg agcggctcta tgggattcac cgtggcatat
    12601 tcaccagccc gaacgttaac ctgaagaatc gtccccccac gtggtgcccg gacggtgaga
    12661 cgatttaaca acgcttggac ttcatcgcgt tccgttcggg ccaaatggag atcagccctt
    12721 gcccgaacca acgcccgctt ggctccttcc gtctcatgcc attttcgctt cacatcatct
    12781 tcactgaccg cccgacggtc tgtgaccgac tgaagccgct tc
//
LOCUS       DAORAQ010000069        12373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_850_length_12373_cov_66.034908, whole genome shotgun sequence.
ACCESSION   DAORAQ010000069 DAORAQ010000000
VERSION     DAORAQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_850_length_12373_cov_66.034908"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /gene="mscL"
                     /locus_tag="PKM72_07945"
     CDS             complement(<1..136)
                     /gene="mscL"
                     /locus_tag="PKM72_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNP60754.1"
                     /translation="MLKEFKEFAMRGNVVDMAVGVIIGGAFGTIAKSLVADVLMPPIG
                     L"
     gene            complement(491..988)
                     /locus_tag="PKM72_07950"
     CDS             complement(491..988)
                     /locus_tag="PKM72_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60755.1"
                     /translation="MLKIEESTPSGACSDRTFLPSLWGKCDLMRTTKNKNFNSNASLG
                     RVVIVSRNDLIRDFLGQIIKLHGYDCEYFEEWPKVLVEKNGQSFDALFIDDHFLDQCK
                     RGLGHGLSYVPGAPLVVILGELPPSEDAGFFHVLKKPLDYRQIGRVMDECLSLKTQRR
                     VSPDE"
     gene            complement(1090..1365)
                     /locus_tag="PKM72_07955"
     CDS             complement(1090..1365)
                     /locus_tag="PKM72_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60756.1"
                     /translation="MLIFTLGIKPEGLEPQIVWNGKDIAPRVAIYSGCEGKDRERIPV
                     LKVVKKAKGTVITVRSYISELSRDVFYQAKFTSDSYGPWQKVALCWL"
     assembly_gap    1873..1972
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2383..4170)
                     /locus_tag="PKM72_07960"
     CDS             complement(2383..4170)
                     /locus_tag="PKM72_07960"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP60757.1"
                     /translation="MSGSLPDPAFLSVFDALIFQKSAERLDHFGDLPSWFPPPGLKQE
                     TGLPPEQLVERSFFLQHFLHEAREWWESGRTGQCKSGLWSEPALFGEDVVLEATAVRH
                     RSLRYVILQRFGPDILPIQPLLQKAREEQLSHRQAVNQHERTEARLGSRLAKSEQERD
                     DVMAVLDGLGLGTIMLNANRDVTFISGKASELLQTDPARMIGRTLVEGFPWKPADSVR
                     VEEMGRLPRSQRQVVPVVMDGKNHSATALEVEVQDDPRDPRKTILLLKDVSEMEALRR
                     QLVGPTQFHNLVGKCPAMRAVYERIGDIAVVDVPVLIDGETGTGKELVARALHQLSPR
                     HEYPFLPVNCAGLTDSLLGSQLFGHKRGAFTGATSDHEGFFESAEGGTLLLDEIGDMP
                     LPIQTTLLRVLQEGEIIRLGESRPRKVNVRVLAATNQPLQELVDRGAFRADLLYRIRV
                     ARLRLPPLRERREDIPLLSTAFFEKSRVAFGKMQVLGIAPETMQRFLRYSWPGNVREL
                     QAALEYALIHCRSENILPTDLPPELCDVFPAVQSSPLLKPNERQRILDALAQTKGKRA
                     QAAKLVGMSRSTFYRRLTELNIAHEESSI"
     gene            complement(4272..4769)
                     /locus_tag="PKM72_07965"
     CDS             complement(4272..4769)
                     /locus_tag="PKM72_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HNP60758.1"
                     /translation="MIQKKICMLGGFAVGKTSLVKRLISGIFSEKYLTTVGVKIDQKS
                     LSVCGQDLLLVLWDLYGDDDFQKVRASYLQGSSGYILVIDGTRKETVDVAGKLHELAL
                     KTIGPVPFVILLNKHDLVNEWVVESPMVSNLSDQACLVSMVSAKTGEGVEAAFSALSE
                     AMLKS"
     gene            complement(4766..6562)
                     /locus_tag="PKM72_07970"
     CDS             complement(4766..6562)
                     /locus_tag="PKM72_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60759.1"
                     /translation="MERTPRSSPLRPSTGYDKGESEDFSALRRLLLSPEQIQLDRLRQ
                     RMDTLVIGPQEVSRVLPDAIRLRAQQDNRLLASLLPITQEALALSIKQSPQLISDSIA
                     PILGSAIRKAISRAMREMFQSLNQTLEYSMSWQGLQWRWEAWKTGRPFVEVVVLHTLR
                     FRVEQVFLIHKKTGLLLNHVMAEGISSQGEVAISGMLTAIQDFVRDSFQQGQEEGLES
                     LRIGDLTVWIEQGSSAILATVIRGTPPVKLRDFLQSTLESLHVQYSDFLKDFHGDTAV
                     FLETRPHLEDCLQAQFVKKRKGLPVVFWVCSGLLLALLGWWAYQATLSHQQWTELLKR
                     IAAEPGIVVTTIQEEDGRTVFHGLRDPLAVDPEQLLEEVGYPPHAAGFHFEPFMALTP
                     EMVRKRAITVLQPPGSVEVSIEGSRIILIGESSHAWIARATRSASAIPGVSEISLERL
                     IDTDVKQFEALQVELEGKAIFFFPGQGRLPDRELVKIQQVKFILQELDEKAMQLGRKP
                     SIDVMGYSSPVGTQPINVSLSERRANNVLEMLNENTFQVLKLSAVGKGVDPDVQSGSG
                     GEQGYSASRRVSFKVVEPGIPLRREVGVRSRP"
     gene            complement(6677..7303)
                     /locus_tag="PKM72_07975"
     CDS             complement(6677..7303)
                     /locus_tag="PKM72_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60760.1"
                     /translation="MSATEPSKKPFKTVAYEVSTSQGTSSSQDGEWSAQSDAGSLDKV
                     RDILFGAQSRDIEKRFAALEDRLFQESSRLREDLTKSVDDLKTHMNQEIQGLVGKLQQ
                     EQDRRVATIQDMGQTVKTVESQISKRIVELTEHASNQFQAMSAEMQRQKHELIEQDQE
                     AMSRMESQFEQAVNELKVEKTDRAVLAEMLMEVALRLKVGAQGIETQS"
     gene            complement(7544..7756)
                     /locus_tag="PKM72_07980"
     CDS             complement(7544..7756)
                     /locus_tag="PKM72_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60761.1"
                     /translation="MDHKNTSEEPNDSEKAEAKEPKPDSPKKIKKELSVVSLPHDNDV
                     MAQEMADMFDEDRVTHKHGNAEPEKD"
     gene            complement(7780..8139)
                     /locus_tag="PKM72_07985"
     CDS             complement(7780..8139)
                     /locus_tag="PKM72_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60762.1"
                     /translation="MTQLSIKQVEERLGEVKCPICKANRFGIDSRTATEDGEWKAICI
                     GCHYMFPVHTDMEFYVQTQPDIPYHLKEIPCPSCRHRGVSLDLRAVLSVRESVYFVTC
                     PGCQHKFPERSHLESFE"
     gene            8444..9274
                     /gene="mutM"
                     /locus_tag="PKM72_07990"
     CDS             8444..9274
                     /gene="mutM"
                     /locus_tag="PKM72_07990"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNP60763.1"
                     /translation="MPELPEVEVILNHLNRRVLGGTIERLTIHRSDIVRTGHDFIPWF
                     QHSTITNIARRGKCLIFTCHRDNETRYLLSEQGMTGLWFFHPALASTPQHIHCRIEIS
                     GVPTSELHYWNPRRFGRLWLFAAPELEAFMQRRFGSDAMNIGAQPFIKLIQSCRGRLK
                     PFLLDQHRLAGIGNIYANEILFRAGVHPQAHGYRLGVSTCQRLYDTMQTVLQEAILAG
                     GSSIRDFRAPDGSQGHFQEAHQVYQKAGLPCSHGCSTLITRMQSERSSFFCSSCQKRR
                     "
     gene            9369..10415
                     /locus_tag="PKM72_07995"
     CDS             9369..10415
                     /locus_tag="PKM72_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNP60764.1"
                     /translation="MRKFKLREGVDLANLFGPHDLHLKMIEGELQVRMAARGDEITLH
                     GQPESVLRAEHILRELADWTRTYYELKPDDISRAILATEEKPDTPLKPYTVSASALPT
                     KKGTVNPKTPNQQAYLEAINQSDLVIAIGPAGTGKTYLAMAMALAALTHKQVSRIILA
                     RPAVEAGEHLGFLPGDLFAKVHPYLRPLYDALYNMMDIDRANRLLERGEIEIAPLAFM
                     RGRTLDDAFVILDEAQNATTEQMKMFLTRLGLNSKAIVTGDITQIDLPDSQKSGLAQI
                     ADILLNIDGIQFVYFSEQDVVRHRLVKEIIKAYDRFEHQLPNNSPHNISSDHSTASPG
                     NHSKSDTNGQRPTR"
     gene            10422..10871
                     /gene="ybeY"
                     /locus_tag="PKM72_08000"
     CDS             10422..10871
                     /gene="ybeY"
                     /locus_tag="PKM72_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNP60765.1"
                     /translation="MAVWLRSHLRRVAVRPTTVRRLTQAVLQQAGYPSANLSLTFVGK
                     TRMQKLNRTYRERDYATDVLAFPMQDVLQSSQAFVGDVVICIPVALAQASRFNNTADE
                     EILRLLIHGTLHLLGYDHETTEREAKRMKRKEQTIFRRLSPVPRLLA"
     gene            10920..11840
                     /gene="ftsY"
                     /locus_tag="PKM72_08005"
     CDS             10920..11840
                     /gene="ftsY"
                     /locus_tag="PKM72_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNP60766.1"
                     /translation="MKWVERLQNGLAKTRQSVQKSLRRLGRGHLDPVALEEVEASLLE
                     ADVGIHTIEQFLEMLKDKTRVFSGTDPTAVLHTLLMDILRKGETEPLEQLIRRGPRPF
                     IILTIGINGVGKTTTIAKLGNQLKQQGLHTLFVAGDTFRAAAIEQLDIWGKRTGIEVI
                     KQKQGSDPAAVVFDGMVAAKARNVDVVLIDTAGRLHTKINLMDELKKIKRIIAREMPG
                     APHETLLVLDGTLGQNAIAQARQFHESLGVTGLALTKLDGTSKGGVVVAIVDELNLPI
                     RLIGVGEGEADLQDFNASAFVEALLPRDPP"
     gene            11926..>12373
                     /locus_tag="PKM72_08010"
     CDS             11926..>12373
                     /locus_tag="PKM72_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60767.1"
                     /translation="MSFSHPILSPAIQVPSFILALAWLWLASASPLQANPLTPIHHKL
                     EIELNPDTHAMTGLDTINLPPSHRANSSVSFILNPQLTIDRVEFNGKLLPVPPPSEGQ
                     TVQPGSTRRVIEIPLPPLDGPDQPAVLTLTYHGQIDDSPTASGGLRF"
BASE COUNT         3194 a         3403 c         2702 g         2974 t
ORIGIN      
        1 agagtccaat agggggcatc aacacatcgg ccaccagcga tttggcaatg gtgccgaagg
       61 cccctccaat aatgacaccg acggccatgt caacaacatt gcctcgcatg gcaaactctt
      121 tgaactcttt aagcattaca tcctccttat tatgagagtg aaagatagaa ccgtttgttc
      181 tccagaatta cacacgtgag ggggtcacaa aaggcacatc atcctctcgt aacacattaa
      241 caaatgcaag atccttcctg gctttctatt cttcgcctaa ccggactttc acccatactt
      301 actccttctg tgacccatgg aacaaacgga tcagcttccg taaaaaaccc tggggcgacc
      361 agcctgtcct cgctaatttc cagttgaaga ccccctctaa aagccccaac tgtcaagatc
      421 aactcggata agtggtcgcc ctcggcaatc tgcacaatga ccaaaatttc aaacagctcc
      481 gctccgacgc ttactcgtct ggagatacgc gtctttgcgt tttcagtgaa agacattcat
      541 ccatgactct tcctatctga cgataatcca acggtttttt cagcacatgg aaaaatcccg
      601 catcctccga tggaggaagc tctcccaaaa tcacgaccaa aggggcaccc gggacgtagg
      661 aaagtccatg gcccaagcca cgcttacatt ggtccagaaa gtggtcgtca atgaataggg
      721 catcaaagct ctgcccattt ttttccacca agacctttgg ccattcctca aaatattcgc
      781 aatcataccc gtgcagcttt attatctgcc caaggaaatc acgaattaag tcattccgac
      841 tgacgatcac gacgcgacct aaagaggcgt tcgaattaaa attcttattt ttcgtcgttc
      901 gcataaggtc acattttccc caaagacttg gcaaaaacgt ccggtcggaa cacgccccgc
      961 tcggagttga ttcctcaatt ttcagcatgt gtgaatcccc atttggaaaa agtccaattc
     1021 tctcctcacg ttcagacaga ttcccctcca tcgcctgact cactgatttc tgatttgtct
     1081 tgtccatttc taaagccagc acaaggcaac tttttgccag ggaccatatg aatccgatgt
     1141 gaattttgct tgatagaaca catcccttga taactcagaa atatagctcc gcaccgtgat
     1201 gaccgttccc ttagcttttt tgaccacttt gaggaccggg atcctttctc ggtccttccc
     1261 ctcacatccg gaataaatgg ctacccttgg ggcaatgtct ttgccattcc acacaatctg
     1321 tggttccagc ccctccggtt ttattcccaa ggtgaaaatc aacatggcga acattccaag
     1381 tgcactgcaa agacccatgg aaaaaactat atgattgttt tgaatcttca ttggttggca
     1441 tccatctacc cattgattac ccatattcat taactttcac taacaataat ggtgccagac
     1501 aaaataccct tacatttcaa ttgcttggat ttttaattta aaaagtttcc cattttgaaa
     1561 catccgattg tctcaaagtg tttcagggaa atttttgaat cacgagggaa agggagaaag
     1621 gaggatgaac cacaggaagg gaaaaacagc aaggaaccga acacctgcga tgctcgggcc
     1681 ctcaattaca acttcacctt acgactcaaa tacctatccg agcgacagga tgtattgcgt
     1741 atccgctttt ttggttccag gagccgggga gttatcgtac cgccaattaa tctgaatagt
     1801 cgaaatgagg tcatcccaga tattcgtacg aattccctgt tggtacgtca cctaccaatc
     1861 actggttttt tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagatattc
     1981 gtacgaattc cctgttggta cgtcacctac caatcactgg ttttttaatg ggagagaaaa
     2041 cctttatgct gatgaaatag cgtcagattg gggagaatcg gccaatgaaa attcaccgcc
     2101 catcgaccgg taaaataatt gacatcttct gaacgtttaa cgttctcgtc aaaatacccc
     2161 ggttcggaaa agaacgatga acgaagattt tgatcttgaa acgtacccag tccaatcaac
     2221 acaccggtaa aatatagaac cgcttcgtca caaaaaaatt ccttttaacc gtcccctagg
     2281 cagatggact gtcgattgta attgatgtcg acgaaaaccc aacacattta tcccggattt
     2341 aatcaccgcc gcaggttcag aggttatccg gaaataccgg aatcagatgg acgattcctc
     2401 atgagcaata ttcaactctg ttaaacgtcg ataaaaggta gaacggctca taccgaccag
     2461 ttttgcggct tgcgcacgtt ttccttttgt ttgtgccagg gcatcaagga ttcgctggcg
     2521 ttcattcggt tttaacaacg gcgatgactg aacagctgga aaaacgtcgc acaactcagg
     2581 aggtagatcg gtcggaagga tgttttcact ccggcaatgg atcaatgcat attccaaggc
     2641 cgcttgtaat tcacggacat tccccggcca ggaataacgt aagaaccgct gcatcgtttc
     2701 cggagctata ccgagaacct gcatcttgcc aaatgcgaca cggctttttt cgaaaaaagc
     2761 cgtacttaac aagggaatat cctctcggcg ctctcgcagt ggaggaagcc gtaatcgtgc
     2821 gacacgaatc cgatagagta aatctgcacg aaaggcaccc ctgtcaacca attcctgaag
     2881 aggctgatta gtggccgcca atacccggac attcactttc cgtgggcgag actccccgag
     2941 acgaataatt tctccctctt gaagcacacg gagcagggtg gtctgaatgg gtaacggcat
     3001 atctccgatt tcatctaaca ataacgtgcc gccttcagcc gattcgaaaa atccttcatg
     3061 gtcactcgtc gctccggtaa aggcgccgcg cttgtgccca aacaattgac tccccaacag
     3121 cgaatctgtc aatccggcgc aattaactgg taaaaatgga tattcgtgac gcgggcttaa
     3181 ttgatgcaaa gcacgggcaa ccaattcttt gccagtcccg gtttccccat caatgagcac
     3241 cggcacatcc accacggcga tgtctccaat ccgctcatac accgcgcgca tggcgggaca
     3301 ttttccaacc agattgtgaa attgtgttgg ccccaccaat tgacggcgca acgcttccat
     3361 ctcgctgaca tcttttaata atagaatggt ttttctcgga tctcgcggat catcttgaac
     3421 ctcaacttcc aacgcggttg cagaatggtt tttcccgtcc atcaccaccg gcacaacctg
     3481 acgttgcgac ctcggcaacc gtcccatttc ctcgacccga acactatccg ctggtttcca
     3541 cggaaaaccc tcaacaagag tgcgtccgat cattctggcc ggatccgttt gcaataactc
     3601 gctggccttt ccactgataa aggtcacgtc acgattcgca ttgagcatga tggtccccaa
     3661 tcccaaaccg tcaagcacag ccatcacatc atcccgttct tgttcgcttt tcgctaaacg
     3721 actgcccagc cgggcttcgg tgcgttcatg ttggttgaca gcctgacgat gtgaaagctg
     3781 ttcttcacgt gccttttgca gcaagggttg gataggaagg atatcgggtc caaagcgttg
     3841 cagaatgaca taccggagag accgatgacg gacggcagtg gcttccagga cgacgtcttc
     3901 tccaaataat gcgggttcgc tccacaaacc ggacttacat tgtcccgtcc tgccggattc
     3961 ccaccattcc cgggcttcat ggagaaaatg ttgaagaaaa aatgaacgtt ccactagctg
     4021 ctcgggaggc aatccggtct cctgtttcaa gccaggcgga ggaaaccaac tgggaaggtc
     4081 accaaaatgg tcgagccgtt ctgcagattt ttgaaaaatt aacgcatcaa agacactgag
     4141 gaaagcagga tcgggtaagg aaccggacat gagtctcacc aatctctggt aatgacgaga
     4201 ggatacggga atctgattga gttcaccgac acatactcac caggcgatgg gtattcgatc
     4261 catggccggc ctcaggattt cagcatcgcc tcggacaacg cggaaaacgc tgcttcgaca
     4321 ccttcaccgg tttttgcgct caccatggat accaggcagg cttgatcact gagattagaa
     4381 accatgggag actcaaccac ccactcgttt accaaatcat gtttattgag cagaatgaca
     4441 aaaggaacag gaccgatcgt cttcagggct aattcatgta acttgcctgc cacatcaacc
     4501 gtttctttcc tagttccgtc tatcaccagg atatatcccg atgacccctg caaatatgac
     4561 gcccgaactt tttggaagtc atcatcgccg tagagatccc aaagaaccag caggaggtcc
     4621 tgcccgcaaa ccgacaaaga tttttgatca atcttgaccc caaccgtggt gagatatttt
     4681 tcggaaaaaa tgccggagat caatcgtttc accaggctcg ttttccctac ggcaaaccca
     4741 cccaacatac aaattttttt ctgaatcacg gtctgctcct cacacccact tccctgcgca
     4801 gaggaatccc tggttccacc accttaaagg acactcgccg actcgccgaa tacccctgtt
     4861 ccccaccgga accggattgt acatcgggat caaccccttt acccacggca gacaatttta
     4921 acacttgaaa ggtattttca ttgagcattt ccagaacgtt gttcgctcgc ctctcactca
     4981 gtgaaacatt gatcggttga gtgcccacgg gactgctata tcccatgaca tcaatagagg
     5041 gtttccgacc gagctgcatg gctttctcat ccaattcctg aagaatgaat ttcacctgtt
     5101 gaattttcac caattcccga tccggcaatc tcccctgacc cgggaaaaag aaaatggctt
     5161 tcccttccaa ttcgacttga agtgcctcaa attgtttcac gtccgtgtca atgagacgtt
     5221 ccaaggaaat ctccgaaacc ccgggaattg ccgaggcgga tcgagtcgct ctcgcaatcc
     5281 aggcatggga actttcccct atcaaaataa tccggctacc ctcaatgctc acctccaccg
     5341 atccgggtgg ctgcaaaact gtgattgccc gcttacgaac catctccgga gtgagggcca
     5401 tgaacggttc aaaatgaaac ccggccgcat ggggaggata tcccacctct tccagcaact
     5461 gttcggggtc aaccgccagc gggtccctca acccgtggaa caccgttcgc ccatcttctt
     5521 cctgaatagt cgtcaccaca atacccggtt cagccgcaat ccgcttcaac aactcagtcc
     5581 attgttgatg actgagagta gcctgatagg cccaccatcc caaaagagcc agaagaagac
     5641 cactacacac ccaaaatacg accggaaggc cttttcgttt ttttacgaat tgtgcttgga
     5701 ggcaatcctc caaatggggt ctggtttcta aaaacacagc ggtatcgcca tgaaaatctt
     5761 tcaggaaatc cgaatattgg acatgaaggg attcgagcgt gctttgaagg aaatcccgca
     5821 atttcacagg aggagtgccc cgaatcacgg tggccaaaat cgcgctggac ccctgttcta
     5881 tccagacagt caaatcgcca atcctgagcg actccaatcc ttcctcctga ccttgctgaa
     5941 acgaatccct tacaaaatcc tgtatggctg tcaacatgcc ggaaatggcc acttccccct
     6001 ggctggatat cccttcggcc atcacatgat tgagtaataa tcccgttttt ttatgaatca
     6061 aaaatacctg ctcgactcta aaccgaaggg tatgcaacac caccacttcc acaaaaggcc
     6121 tgcctgtttt ccacgcctcc caacgccact gaagtccttg ccaggacata ctatattcta
     6181 aggtctggtt gagagattgg aacatttccc gcatggcacg agaaatggcc ttccggatgg
     6241 cggaccccaa gataggtgcg atggaatcac taatgagctg gggagattgt ttgatggaaa
     6301 gagccaatgc ttcttgtgta atcgggagca gagacgccaa taaccgatta tcctgttgag
     6361 cccgaaggcg aatggcatcg ggcaggacac gactcacttc ctgcggccct atcaccaacg
     6421 tatccatgcg ttgtcgtaaa cgatctaatt gaatttgctc gggagacagc aacaagcggc
     6481 gaagggccga aaaatcttca gactctccct tgtcatatcc cgtcgatggg cgaagaggag
     6541 acgaacgagg cgtacgttcc ataattggcc ttcaggagga agctgccatt tttctgttag
     6601 agagaggggg aaaaaacacg tgaaattgat cgtcagcctt tgccatggga ggtatacccc
     6661 aatagaattc aaggcttcat gattgggtct caataccctg ggcccccact tttaggcgca
     6721 aggcgacttc catcaacatt tcggccaata ccgcccgatc ggttttctca accttcaact
     6781 cattgactgc ttgttcgaat tgagactcca ttcggctcat ggcctcctga tcctgctcaa
     6841 tcagctcatg tttttgtcgc tgcatttctg cgctcatcgc ctggaactga ttcgaagcat
     6901 gctctgttaa ttccacaatc cgtttactta tttgagattc aaccgtcttg actgtttgtc
     6961 ccatatcctg aatggtggca acacgtcgat cctgttcctg ctgtaatttc ccgactaacc
     7021 cttggatttc ctgattcatg tgcgttttca gatcatcgac agatttggtc aggtcctctc
     7081 gtaggcgact gctttcctga aacaaccgat cctccaatgc agcgaaccgt ttttcaatgt
     7141 cccgactttg ggcaccgaaa agaatatccc taaccttatc caggctccct gcatccgact
     7201 gtgccgacca ttctccgtcc tgggaagacg aagttccctg agaggtcgag acttcatacg
     7261 ccacggtctt gaatggtttt tttgagggtt cagttgcact catagttgtc actccacgag
     7321 taggtcaata acttctacgt cctagggaac accccttaag cgaagatatc ctaacattga
     7381 taaaaaaagg gaacaagacg gaaagttaca cagtggaagc ctctatggct aacccaaaca
     7441 gcgtaactct ctattgagaa accgtaagga ataaaaccac gatagagggg aatgggaaaa
     7501 gaagttcagg agagggagaa acgtacgatt gttctttttt ttgctaatct ttttccggct
     7561 cagcattccc atgtttatgc gtgactcgat cttcgtcaaa catatccgcc atttcttgag
     7621 ccatcacatc attatcatga gggagagaga caacagacaa ttcttttttg atttttttgg
     7681 gagagtcggg cttgggttct ttggcctcgg ctttttcaga atcgttgggt tcttcagatg
     7741 tatttttgtg atccatgatg acaatctcct ctacaatgat tactcaaacg attccagatg
     7801 tgaccgctca gggaacttgt gttggcaacc gggacaagtc acaaaataga ccgattcacg
     7861 aaccgagagc accgccctca aatccaggga caccccgcga tgtcgacaag atggacaggg
     7921 aatttcttta agatgatagg gaatatccgg ctgtgtctgt acataaaact ccatatcggt
     7981 atggacggga aacatataat gacacccgat acaaatcgct ttccattccc cgtcttccgt
     8041 tgccgtcctg gaatcgattc caaagcgatt ggccttgcaa attgggcact tcacttcccc
     8101 taaccgttct tcaacctgct tgatgcttaa ttgtgtcatt cttcttcctt atccactctg
     8161 gtgaatatgc cctggtcaaa gtataggctt cctgccagag atcccgcaac caaaccctct
     8221 aaattctcta cacgggccag gcctcccgtt cagagattag ctcaatccca tccgatcaca
     8281 cgtgagccgg agtctattgc cttgcccgaa ttagacacct caccaataaa tgaacatggc
     8341 aatacccagt gctacgtcga atctgataca accttccaga ccaaattaag gaaaaactga
     8401 cccacgtgca ctcaacctat tgagtttccc cgcgtaatct tctatgcccg agctccctga
     8461 agtcgaagtc atcctcaacc atctgaaccg gcgtgtccta ggtgggacca ttgaaaggct
     8521 caccattcac cgttcggata ttgtccgcac cggacatgac ttcattccct ggtttcagca
     8581 ctccacgatc accaatattg ccaggagggg taaatgtctc atcttcacct gccatcgaga
     8641 caacgaaacc cggtatcttc tttcggagca gggcatgacc ggactatggt ttttccatcc
     8701 agccttggcc tcaactccac aacatatcca ttgccgcatc gagatttccg gagttccgac
     8761 cagtgagttg cattactgga atcctcgacg atttggccgg ctctggctgt tcgccgcacc
     8821 ggaattggaa gctttcatgc aacgccgatt cggctctgac gccatgaata tcggtgccca
     8881 acccttcatt aagctcattc aaagctgtcg aggtagactg aaaccctttc tcctcgatca
     8941 acatcgcctc gccggaattg gaaacattta cgccaatgaa attctatttc gcgccggagt
     9001 gcacccccag gctcacggct atcgactggg cgtctcaacc tgtcaacggc tctatgacac
     9061 catgcagacc gtattacagg aggccattct tgccggaggc tcttccatca gagactttcg
     9121 tgctcccgat ggatcacaag gccactttca ggaagcgcat caggtctatc agaaagcagg
     9181 attgccatgt tcgcatgggt gttcaaccct catcacgcgc atgcaaagtg agcgcagctc
     9241 atttttttgt tcgagctgcc aaaagagacg gtaatccttc catttcgttc cttgagccaa
     9301 ccttaaacct ttgctagaat ctgtctatga gtgttcttgt ggctaaccgg aggtgaatcg
     9361 aatcctacgt gcgaaaattt aaactacgag aaggggtcga tttagcgaat cttttcggcc
     9421 cgcatgatct gcacctcaaa atgatcgaag gggaactcca ggtccgaatg gcggcccggg
     9481 gggatgaaat cacccttcat ggtcaaccgg agtcagttct gcgggccgaa catattttgc
     9541 gggaattagc cgattggact cgaacctatt acgaactgaa accggacgac atttcacgag
     9601 ccatactggc gacagaagaa aaacctgaca ctccgctcaa accctatact gtttcggcaa
     9661 gcgcgcttcc gacaaaaaaa ggaaccgtta acccgaaaac ccccaatcag caggcctatc
     9721 tcgaagccat taatcaatca gatctcgtca ttgccattgg accggccggg acagggaaaa
     9781 cctatctggc catggccatg gcgttggcag cgctcacgca taaacaggtc agtcggatca
     9841 tcctcgcacg gccagccgtg gaagcagggg aacatctcgg ctttctcccg ggagatctct
     9901 ttgccaaagt gcacccctat ttacgtcccc tatacgatgc gttgtacaac atgatggaca
     9961 ttgatcgggc caatcggcta ctcgaacgtg gtgaaattga aatcgctccc ctggcgttca
    10021 tgcggggacg aacgctcgac gatgcctttg tcatcctgga cgaagctcaa aacgccacca
    10081 cggaacaaat gaaaatgttt ttaaccagac tcggactcaa ttccaaggcc attgtgaccg
    10141 gtgatatcac ccaaatcgat cttcccgatt cacagaaatc cgggttggcg cagattgccg
    10201 atattctcct caatattgac ggcatacagt ttgtctattt ttccgaacag gatgtggtcc
    10261 gacatcggtt ggtcaaagaa attatcaaag cgtacgatcg gtttgaacac caactgccca
    10321 acaatagccc gcacaacatc tcttccgacc attcgaccgc atcgcctgga aatcattcaa
    10381 aatccgacac caatggccag aggcccacgc ggtaaaacac gatggctgtg tggcttcgtt
    10441 ctcacctccg gcgagtagcc gttcgcccca cgacagttcg ccgactgacg caagctgtgt
    10501 tacaacaggc agggtatcct tcggccaatc tgagcctcac cttcgtcggc aagactcgca
    10561 tgcagaaact taaccggacc taccgggagc gggactatgc gacagatgtg ctagccttcc
    10621 ccatgcagga tgtcttacag tcgtcacagg cattcgtggg agatgtcgtg atttgtatcc
    10681 ccgtggccct ggctcaagcc tcaaggttta acaacacggc cgatgaagaa atcctgcgtt
    10741 tgctcatcca tggcaccctt cacctcctgg ggtatgacca tgaaacaacc gaacgggaag
    10801 ccaaacggat gaaacgaaaa gagcagacaa ttttccgtcg tctttcgcct gttccccgtc
    10861 ttctcgcata acataggaca gggatcattc acttgccatg atattcgggt acatacacta
    10921 tgaaatgggt tgaacgtctt cagaatggat tagcaaaaac acgccagtcc gtgcaaaagt
    10981 cattgcgtcg cctggggcgg ggccacttgg accccgtcgc actggaagag gtcgaagcca
    11041 gtctcctgga agccgatgtc ggcatacaca ccatcgagca attccttgag atgctgaaag
    11101 ataaaactcg ggtgttcagc gggaccgatc cgacagcggt cctccatact cttctcatgg
    11161 acattcttcg taagggcgag accgaaccgc tggaacaact cattcgacgc ggcccacgtc
    11221 cctttattat cttgaccata ggcatcaacg gtgtcggcaa aaccacgacc attgccaaac
    11281 tcgggaacca gttgaagcaa caagggttac acacactctt tgtcgccggc gacactttcc
    11341 gggctgcggc aattgaacaa ttggatattt ggggaaagcg cacaggcatc gaggtgatca
    11401 agcagaagca gggctccgat cctgccgccg tggtgtttga tggcatggtc gccgcgaaag
    11461 ctcgaaatgt ggacgtcgtt ctcatcgata ccgccgggcg gctgcacacc aaaatcaatt
    11521 tgatggatga attgaaaaag atcaaacgaa tcattgcgcg ggaaatgccc ggcgcccccc
    11581 acgaaaccct cctcgtcctg gatggcacat tggggcagaa tgccattgca caagcccgac
    11641 aattccatga gtcactcggc gtcaccggat tagccttgac caaactggat ggaacctcca
    11701 aaggcggagt ggtcgtggca atcgtcgacg aactcaatct tcccattcgt ttgatcggcg
    11761 taggagaagg agaagcggac ctccaggatt ttaacgcttc agcgtttgtc gaggccttac
    11821 tcccaaggga ccccccctag cctaaaacag cacggccttc ttcctcccaa aatattctgc
    11881 cggaaagcga aactgtattc atcaattcaa caaggaaatt atcttgtgag tttcagtcac
    11941 ccaatccttt ctccagcaat tcaagtccca tcatttatcc tggcgttggc atggctgtgg
    12001 ctggcgagcg cgagtccgct ccaagccaac ccattgacgc ccattcacca taaattagag
    12061 attgaactga atcctgacac ccatgccatg accggattgg acaccatcaa tcttccacca
    12121 tcccatcgcg ccaactcatc cgtctccttc atcctgaatc ctcaactcac aatcgatcgg
    12181 gtcgaattca acggcaaatt gcttccggtc cctcccccct ctgaaggcca gaccgtgcag
    12241 ccgggatcaa cgcgtcgggt tattgaaatt cccctgcctc cccttgacgg tccggaccaa
    12301 ccggcggtcc tcacactgac gtaccatggg cagattgatg attctccaac agcctcgggc
    12361 ggacttcgct ttg
//
LOCUS       DAORAQ010000070        12322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_852_length_12322_cov_63.091302, whole genome shotgun sequence.
ACCESSION   DAORAQ010000070 DAORAQ010000000
VERSION     DAORAQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_852_length_12322_cov_63.091302"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PKM72_08015"
     CDS             <1..1336
                     /locus_tag="PKM72_08015"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNP60768.1"
                     /translation="AYYDALTKLPNRSLFLDRLAWFIQRAERHQENLVLLHITLNQFK
                     RLSNTFGPEISDQLITQIAERMSSCMRRSDVFGRGIPDSSELDSLFRVDGDEFSLLCP
                     TMAHAEHATKLASRILKVMESPFNANGTEVYIAPSIGIASFPADAEDLTTLIQCAVGA
                     SAQANFHGKGGFNFYSSELNAHSLERLRMEADLRHAIEEDQLLLYYQPKVEVKSGSII
                     GVEALVRWQKPDGTLIFPDQFIPLAEETGLIIPLGEWVLKEACAQLARWQAQGIWIQV
                     AVNVSAKQFNDGYLVDLVRETLKNRGVDAKHLTLELTESLLMGNAEQAVETLNHLMAL
                     GPKISMDDFGTGYSSLSYLKRFPIHELKIDRSFLIDITHNPEARALVSAMIYLAHEFS
                     LTVVAEGVENQEQLDLLTTLNCDQYQGYFFSRPIRPEALAPMLHVEGPSVKA"
     gene            1724..2956
                     /locus_tag="PKM72_08020"
     CDS             1724..2956
                     /locus_tag="PKM72_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP60769.1"
                     /translation="MNTVIANYGNIFVGRQPILGPNLKTIGYEVLYRNCESDSAIIHN
                     EEIATAQVLLNTYLDIGLEHVVGTHLAFLNIPKQFLLDRYCEALPKNRVVLEILETVE
                     PTQQVIEAIASLSEQGYTIALDDFVFHDRFRPFLELADIVKIDVLGKSSEQLQQETRQ
                     IRDYRARLLAEKVETREAFETCKQLGMFYFQGFFFYRPDIIRGREIPGNRIALLELLG
                     KIQDPNISFECLVEFIRNDLSLSYKILRYVNSASVGLPRRVESIEQAACMVGIDRIRT
                     WASLIVMAIGKDQPMEMLVIALVRAKMCERLGQQLGADSPEKYFTMGLLSVLEALYGS
                     SMEKLVGTLPLPDEILEALILEKGPMGAALSSVKAYEKGQWLELKKLQLPPSTIREYY
                     VEAIDWANHFSPMIAEPA"
     gene            3173..>3752
                     /locus_tag="PKM72_08025"
     CDS             3173..>3752
                     /locus_tag="PKM72_08025"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNP60770.1"
                     /translation="MTFIPTSEISSVSSSAKPSRRRNLPPWFKVRFRPGPHYQEIRQL
                     MDTHRLHTICEEARCPNIWECWNNRTATFLILGDICTRRCHYCSVTTGRPSAVDREES
                     QRVADAVKLLKLRHAVVTSVNRDDLPDGGAALFAETIQRIRHENPACTVEVLIPDFQG
                     EQTDLEKVMQAKPDILNHNIETIPRLFPTIRPQ"
     assembly_gap    3753..3852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3853..4242
                     /locus_tag="PKM72_08030"
     CDS             <3853..4242
                     /locus_tag="PKM72_08030"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNP60771.1"
                     /translation="LNHNIETIPRLFPTIRPQGKYQRSLRILQWAKTMGGRTKSGLMA
                     GLGESNDEIRQVMRDLREVDCDILTIGQYLQPTLQHAAVSRYYTPEEFEEFKQEGMAL
                     GFQHVESGPLVRSSYHAEEQTHGRSCP"
     gene            complement(4702..5043)
                     /locus_tag="PKM72_08035"
     CDS             complement(4702..5043)
                     /locus_tag="PKM72_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60772.1"
                     /translation="MKSLNKFCVLLLVMGCQGVVPVFAMEGEPMAQPPEVRQELQTNS
                     PTTTARYRNVEGKLQAIQGDMFIIEGASAEQQVKIQVGKDTAFPNGQKEVGQPVNALV
                     SAQDGHALIIQ"
     gene            complement(5432..6118)
                     /locus_tag="PKM72_08040"
     CDS             complement(5432..6118)
                     /locus_tag="PKM72_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNP60773.1"
                     /translation="MTRSNDHEKDEYHEFSFGVIFNFKKGDLSMQGFKNFMMVVGLVA
                     LVTGGTGSLAGADSGSEHNPHGKQPHAGMGYGNGDHEGMGRHGGLSTLAMKEQLGLSD
                     EQVNQLRPLELEYRKTMIQNGADLRVAMMDLGMFTDAKKTDRAAIAGKVDEISLLQKK
                     MMMYRVEVLLKVKEVLSPEQYEQFRSRLRVQMEGMAHHGGGKQDVEEHHGKYPGKSHG
                     QDGEIEKNHP"
     gene            6241..7386
                     /locus_tag="PKM72_08045"
     CDS             6241..7386
                     /locus_tag="PKM72_08045"
                     /inference="COORDINATES: protein motif:HMM:NF010639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNP60774.1"
                     /translation="MQDYLPGLAGVPAAKSTISYVDGVQGILEYRGIPIEELATRSSF
                     TETAFLLLFNRLPTQAELARFHEDLTHHRRIKYRITDLLKCLPEHGHPMDALQAAVAA
                     LGMFYPVPDVRDDNTRYWTAVRLIAKLPTIVAAFARLRHGDEQIQPRDDLSFAENFLY
                     MLTEKVPAPDMARMFDICLILHAEHTMNASTFSGLVTASTLADPFTVIASAIGTLEGP
                     LHGGANEAVIHMLEEIATIEAIPAFLNHKFSLKQKIMGFGHRIYKVKDPRAHVLQEFA
                     QQLDNQPDGNSLFALARAVEQEMEKRVGPKGIRPNVDFYSGIIYHRMGIDTDLFTPVF
                     AMARVAGWLAHCFEQYQDNHLFRPDQIYEGPRHRSYQSVETRENIST"
     gene            7453..9171
                     /locus_tag="PKM72_08050"
     CDS             7453..9171
                     /locus_tag="PKM72_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNP60775.1"
                     /translation="MMNSAFSYDVLVIGAGLAGMRAAIAAHNQGASVALLTKVHPVRS
                     HSNAAQGGINAALTDRGDMWQDHAFETVKGSDYLSDQDAVEVLAQEAGQDIIALEHMG
                     VIFNRNEEGHLGTRRFGGQKRARTFFVSDFTGQAMLHVLYEQTMQAKIPVYEEWFVTS
                     LVKDDQGRCAGVVAFEIRTGTFALFRARSVIMATGGLGRVYEPSTNALICTGDGMAVA
                     YRAGAPLMDMEMVQYHPTTLKGKGLLITEAARGEGAYLLNSKGERFMERYAPNMLELA
                     SRDVVSRAEQIEINEGRGIKGCVLLDCRHLGRAFLQDRLGQIYAEAKTFANIDLAEEP
                     LPIRPGMHYQMGGIKTDTEGRCWDVQGQWTGVPGLFAAGETACVSLHGGNRLGANSLL
                     DTVVFGRRAGTCAAEYANTNPPPTIPDSAMDKDKHLVANIVSRKGPGERAAALRLEMG
                     TTMNRHVSVFREEEGMETALHHITTLKTRWPDITIQDKGQVFNTGLIAALELGFMLDC
                     AEAIIVGALTRQESRGAHFRTDFLDRDDEHWLKHVLLYHRPDSPPHVEYLPVRITQWQ
                     PQIRVY"
     gene            9287..10246
                     /gene="sdhB"
                     /locus_tag="PKM72_08055"
     CDS             9287..10246
                     /gene="sdhB"
                     /locus_tag="PKM72_08055"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNP60776.1"
                     /translation="MHTTLRIRRFNPEHDHPSSYYQEYDLEIDPSDSVLDGLIKIRET
                     IDDSLTLRCSCRGAICGSCGMRINGRATLACKTKMISMASEGETIQVEPMNNMPVIKD
                     LVTDMAPFWAKIRQIQPWLQPQGLPPTAEYTASPESMSHLSGVMSCIMCGVCVSDCTV
                     LEVDKTFIGPAALAKAYRFVADPRDAATSQRLNFLNQPSGMWDCTRCMECIQVCPKGV
                     GPMDRIMALRAKGLAKKVPSTCGSRHAEVFTDIIKHKGILDEPMLAIRTFGLKNIGRL
                     LAMIPMVLQSLLKGKVPPLGPLHRPIPGIQHIQRLFKQVRITR"
     gene            10243..11121
                     /locus_tag="PKM72_08060"
     CDS             10243..11121
                     /locus_tag="PKM72_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNP60777.1"
                     /translation="MKYAFFPGCVSKGACPELYQSVMQVYPRLGIELAEMTTASCTGA
                     GVLQEKDPRLGDALNARTFALAEQQGLPIMTICSTCQGVMSQANHRLTTRPHYLQEMN
                     DLLREEGLTYRGTTTIKHFLWILLEDVGEETLRKHITRPLSGLRAAPFYGCYIQRPTD
                     ALEFDRNPDRGKSLERIIEILGAEVVDFPGKTRCCGFPILTINEKNSLTMVATHTRDA
                     KSHGADIMVTPCPLCHLNLDGMQTNAARQEQTSIDLPILHLPQLLGLAMGLDTKSLGL
                     QRNLISPESVLAKLSI"
     gene            11220..11438
                     /locus_tag="PKM72_08065"
     CDS             11220..11438
                     /locus_tag="PKM72_08065"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNP60778.1"
                     /translation="MSTKTKDILNQALGLPPVEKAHLVDCLLASLDKPDQAIDNLWRE
                     EVEKRVEAYQSGKILSVSLQQALSKYQR"
     gene            11438..11734
                     /locus_tag="PKM72_08070"
     CDS             11438..11734
                     /locus_tag="PKM72_08070"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNP60779.1"
                     /translation="MEIRLLEIAETELDEAVTFYNSEQPELGADFIVEFIHSLERIKT
                     YPKAWNPFTQNTRRCQLRRFPYGIVYQILEGEILVVAISHLHREPGYWKDRITP"
BASE COUNT         3126 a         3286 c         2873 g         2937 t
ORIGIN      
        1 tgcctactat gatgccttga cgaaattgcc gaaccggagt ttgtttcttg accgactggc
       61 atggtttata cagcgagcgg aacgacatca ggagaacctg gtgttgttgc atatcacact
      121 caatcagttt aagcgcctct ccaatacctt cggcccggaa atctcagatc agctcatcac
      181 gcagatcgcg gagcgaatga gttcatgcat gcgccgatct gatgtatttg ggcggggaat
      241 acccgattca agtgaattgg atagtctgtt tcgcgtggat ggggatgaat tctcgttatt
      301 gtgtccgacg atggctcatg cggaacatgc gacgaagtta gcctcccgta ttctgaaggt
      361 catggaatct ccgttcaatg ccaatgggac cgaggtctac attgccccaa gcatcggtat
      421 tgccagtttt cctgccgatg ccgaagattt gaccaccctc atccaatgcg ctgttggggc
      481 aagcgcccag gccaactttc atggaaaggg aggtttcaat ttttattcga gcgaactaaa
      541 tgctcatagc ctggagcgtc tgcgcatgga agcagacttg cgtcatgcca tcgaggaaga
      601 ccaactcctg ttgtattatc agccgaaggt tgaggtgaaa agcgggtcca tcatcggagt
      661 cgaagcgctg gttcgatggc agaaaccgga cggtaccctc atctttccgg atcaatttat
      721 tcccttagcc gaagagaccg gcctgatcat ccctttgggg gaatgggtgc tgaaggaagc
      781 ctgtgcgcaa ttggctcgct ggcaggctca ggggatatgg attcaggtcg cggtgaatgt
      841 atcggccaag caattcaatg acggctatct agttgatttg gtgagggaga ccttaaagaa
      901 caggggggtc gatgcaaagc atttgaccct cgagctgacg gaaagcctct taatgggaaa
      961 cgccgagcag gccgtggaga ccttgaacca cctgatggcg ttgggaccaa agatctctat
     1021 ggatgacttc gggacaggct actcctctct cagctatttg aaacgttttc ccattcatga
     1081 gttaaagatc gaccgctcct ttctgatcga tattacccac aaccctgaag cccgggcctt
     1141 ggtctcggcc atgatttatt tggcgcatga gttttcattg accgtcgtcg ccgaaggggt
     1201 ggaaaatcag gagcaacttg atcttcttac tactctgaat tgcgaccaat accagggtta
     1261 tttctttagc cgccctatcc gacctgaggc tctggccccg atgctccatg tggaaggtcc
     1321 ttccgtcaag gcataacctt cctccccaaa taattcgaat tgctcgcaat cgactcccct
     1381 tctctccgtc accacctcct cttgttacct ttcaaaagtt cacacacatc tgacaacaac
     1441 cggctggaac ctcaggaggt tcaacgatgt taacgaccct acaatccccg gattccgggc
     1501 cccaacctaa aaaaatgaga tttcccagtt tcaacaagct acttccactt tctttcgtgg
     1561 actttcatga atttcaaaga tttctcaatt ctttcttgtg aaatgccgac aataatgaaa
     1621 ggaaacccct atcagcctgg aaattggaga tgagaataat aggcaccacg gatatgcctt
     1681 ccttcgcaat acacatcaaa gcccctaatc ttcgcctcta gccatgaaca ccgtcattgc
     1741 caattacggg aatatcttcg tcggtcgcca accgattttg ggcccgaatc tgaagaccat
     1801 tggctatgag gtcttgtatc gaaactgtga atccgactca gccattatcc ataatgaaga
     1861 aatcgccact gcccaggtcc tcttaaatac ctatctcgat attggcctgg agcatgtggt
     1921 aggaacccat ctggcttttt tgaatattcc caaacaattt ttattagata gatactgtga
     1981 ggctctccca aaaaaccggg tggtcttgga aatattggag acggtggaac ccacccaaca
     2041 agtcattgaa gccatagcat ctctctctga gcagggctat accattgcct tggacgattt
     2101 tgtcttccat gatcgctttc gaccgtttct ggaattggcc gatattgtaa aaatcgatgt
     2161 cttaggaaaa agctccgaac aattacaaca agagacccgg caaatccggg attaccgcgc
     2221 acggcttctc gctgaaaaag tggaaacacg agaagccttt gagacctgca agcagcttgg
     2281 tatgttctat tttcaagggt ttttctttta tcgaccagat atcattcgcg ggcgtgaaat
     2341 tccggggaat cgtatagctc ttctggaatt attagggaaa attcaggacc cgaatatctc
     2401 ctttgaatgt ctggtcgaat ttatccgaaa cgacctttct ttgagctaca agattcttcg
     2461 atatgtaaat tcggcgtctg tcggacttcc caggcgggtg gagtccattg agcaagccgc
     2521 ctgtatggta ggcatcgacc ggatacgcac ctgggcttct ctcattgtaa tggccatagg
     2581 gaaagaccaa ccgatggaaa tgctggtgat cgccttggtg cgagcaaaaa tgtgtgagag
     2641 actaggacag caacttggcg ccgattcccc ggaaaaatat ttcaccatgg gccttctttc
     2701 ggtcttagaa gctttgtacg gatcttccat ggaaaaactg gtcggcaccc ttcccctccc
     2761 ggacgagatt cttgaagcct taatcctgga gaagggacca atgggagcgg cattgagcag
     2821 cgtgaaagcc tacgagaaag gtcaatggtt agaactcaaa aagctccaac tccctcctag
     2881 cacgattcga gaatactatg tggaagccat tgattgggca aaccacttct cccccatgat
     2941 tgctgagcca gcatgacatc ctgacaaagc cacccatctg ctacccctcc gctgacttca
     3001 cccatccata gggtacaaga ttccccacgc tcctttagga ttgggcgatc ttttggagaa
     3061 tgtaggcatc cttatactca catgttagaa tgagacatgc ctgtctggtg catcccctac
     3121 tcaaagccac tttaggacac attcacaggt catcataaac tccaagaggc tcatgacatt
     3181 catccctact tcagaaattt cgtccgtctc ttcttccgca aagccctccc gaagaagaaa
     3241 ccttcctccc tggttcaaag tccgtttccg gcctggaccc cattaccagg aaatccggca
     3301 actgatggat acgcatcgac tgcataccat ttgtgaagag gcacgatgcc cgaatatttg
     3361 ggaatgttgg aataatcgga cggccacgtt tctcatcctt ggagatattt gcaccaggcg
     3421 ttgccattac tgctcggtga ccacgggacg cccgtccgca gtcgaccgag aggagtctca
     3481 gcgggtggcg gatgccgtca aactcctaaa actccggcat gcagtggtaa cctcggtcaa
     3541 tcgagacgat ctccccgatg gaggggccgc actatttgcg gagaccattc aacgaatccg
     3601 ccatgaaaat ccggcatgca ccgttgaagt gttgatcccg gattttcagg gcgagcagac
     3661 tgatctggaa aaagtgatgc aggccaagcc ggatatattg aaccataata ttgagaccat
     3721 ccctcgtctc ttccctacaa ttcgtccaca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnttgaacca taatattgag accatccctc gtctcttccc tacaattcgt
     3901 ccacagggga aataccagcg atcgctccgg attcttcaat gggccaaaac catgggagga
     3961 cgaactaaat ccggactgat ggcagggtta ggggaatcaa acgatgaaat ccggcaggta
     4021 atgcgggatc tccgggaagt agattgtgac attctgacca tcggccaata ccttcagccg
     4081 actcttcagc atgcggcggt ttcgcgatac tatactccag aggaatttga agaattcaaa
     4141 caggaaggaa tggccttagg atttcagcac gtggaatccg gcccgcttgt tcgaagttca
     4201 taccacgcag aagagcaaac acacggcaga tcctgcccct aattccctct taggtcccaa
     4261 gccttttgat cttcggggcc gcagcaggcc tcggtcagac cggttgatgc atgttcaacc
     4321 gggaaacagg cttgtctgac ttgaataaag aagaattctg cttctgggcg agggaatcca
     4381 aatacacatg ttcgcaaaga ttccgcagag tcacgaaatc aaaagcagta tattcttgat
     4441 agcacgtttc acatttcatc attggccttc tctccaataa tttgttacta tgcacccatc
     4501 aatctaaaat tagcggaagg acgaacactt cgaaccacac accgtgactc ccccaagtga
     4561 caaagtgaat aattctgaaa tgttcaccct tcccgcattc atggcatcga ggataatatt
     4621 taaatcccta cccgatgctc tcttcccata aaattctacc tcataccttc agcccagggc
     4681 atggaaatcc cgagctgccg gttattgaat aatcaacgca tgtccatcct gggccgagac
     4741 cagggcatta acgggttgcc cgacttcttt ttgcccattg ggaaaggcgg tatctttccc
     4801 cacctggatt ttaacctgtt gttcagctga cgccccttcg atgataaaca tatctccctg
     4861 gatggcctga agcttccctt ccacatttct ataccgtgcc gtcgttgtcg gtgaattcgt
     4921 ctgtaattcc tgtcgaacct caggtggttg agccatcggt tccccctcca ttgcaaaaac
     4981 cggcacaact ccttggcagc ccatcaccaa taacaagaca caaaatttat tcaatgattt
     5041 catgacagcc tccttccgca acaaataaat gcgacgaagt acagtatcgc gtttcattgc
     5101 tgaccttact caatagagga cattgcaagg ctcattccct accatgccat gtttttattc
     5161 atgaacaaca tcaaatggtt agaagttttc ctgtggcaat ccgcatcagg tctggcatta
     5221 agatgtgcag gtaatcgcct acaactttcc ctaacaccct gtagggagat aaatgcagct
     5281 tgggggattc tgaaataacg ccaggtccct tctccggttg gaagaaggga cctaggtgat
     5341 caatgctttg ctcaatgacc tcaaacctcg atcctcactc tcaggtcgta agaaaccgaa
     5401 ggagatctca tcaagaggaa cactcctcta ttcagggatg gtttttctca atctcaccat
     5461 cttggccatg agatttgcct ggatacttgc catggtgttc ttctacgtct tgttttcccc
     5521 ctccatggtg cgccatcccc tccatttgca cacgcagccg tgaccggaat tgctcatatt
     5581 gctctggaga caacacctct ttaactttta gaagaacctc aacacgatac atcatcatct
     5641 ttttttgcaa caggctaatt tcatcgacct tgccggcaat cgcggccctg tccgttttct
     5701 tcgcatccgt gaacatcccc aaatccatca tggcaacccg taaatcggcc ccattctgaa
     5761 tcatggtctt ccgatactcc agctcaagcg gacgcaactg attcacctgt tcatcggaaa
     5821 gacccaactg ctccttcatg gccaaggttg aaagaccgcc atgccgaccc atgccctcat
     5881 gatcgccatt cccatacccc attcccgcat gaggctgctt cccatgggga ttgtgctcac
     5941 tcccggagtc agctccagcc aagctccccg tcccccccgt gacaagagcc accaatccca
     6001 cgaccatcat gaagtttttg aaaccctgca tacttaaatc ccctttcttg aaattaaaga
     6061 ttacaccaaa cgagaactca tgatattcgt ctttctcatg gtcattactt cgggtcatcc
     6121 tgattttcca agaaaagtat agcaaattaa ccaggaaagt tggggtggag ttcacgggca
     6181 cggtatacta aattattcgc aagtatacgg atgtaacatc aaagagagag agaaggtgac
     6241 atgcaagatt atttgccagg cctagcaggg gttcctgcgg caaaatcaac tattagttat
     6301 gtggatgggg tccaggggat tctggaatat cgtggtatcc ccattgagga acttgctacc
     6361 cgaagcagct tcaccgaaac ggcattttta ttattattca accggcttcc cactcaagcc
     6421 gaactcgccc gatttcacga ggacctcact caccatcgcc ggattaagta tcgcatcacc
     6481 gatctcctga aatgcctccc ggagcatggc cacccgatgg atgccttgca agcggccgtg
     6541 gccgctctgg gtatgtttta tccggtaccc gatgtccggg atgacaacac ccggtattgg
     6601 accgccgtcc gcctcatcgc caaactcccc accatcgtcg cagcatttgc ccgtctccgt
     6661 cacggtgatg aacagatcca accccgtgac gacctttcct tcgccgaaaa ctttttgtac
     6721 atgctcaccg aaaaagtccc tgccccggac atggccagga tgtttgatat ctgcttgatt
     6781 ctccatgccg aacacacaat gaacgcctcg acatttagtg gactggtgac cgcctccaca
     6841 ctggctgatc cgtttacggt aatcgcctca gccattggca cgttagaagg cccgttgcat
     6901 ggcggcgcca atgaagccgt cattcacatg ctggaggaga ttgcgaccat tgaagccatt
     6961 ccggcatttc tgaaccacaa attttcgtta aagcaaaaga ttatggggtt cggtcatcgg
     7021 atatataagg tgaaggaccc ccgcgctcac gtgttacaag agttcgcaca acaattggat
     7081 aaccaaccgg atggcaattc cttatttgct ctagccaggg cggttgaaca ggaaatggaa
     7141 aaacgagtgg gacccaaagg cattcggccc aacgtggatt tttactccgg catcatttac
     7201 catcgtatgg gcatcgatac cgatttattt actcctgtct ttgcgatggc cagggtggcc
     7261 gggtggttag cacactgttt tgaacaatac caggataatc acttgttccg gcctgaccag
     7321 atttatgaag gcccccgcca tcgttcttat caatcagtgg agacccgtga gaatatttcc
     7381 acctagtcgg gtcacgaacc cggccgtcgt catcgctgtg cggatccaag tgccacgcat
     7441 cagtacttga gaatgatgaa ctctgccttt tcttatgatg tgctcgtcat tggtgccggc
     7501 ctggcaggca tgcgcgccgc tattgccgcg cataaccaag gggcttctgt cgcccttcta
     7561 accaaagtcc accccgtgcg cagtcattcc aatgccgcac aaggcggtat taacgccgcc
     7621 ctgacggacc ggggtgacat gtggcaagac catgccttcg aaacagtgaa aggcagtgat
     7681 tatctaagcg accaggacgc cgttgaggtt ctcgcccaag aagccggcca ggatattatc
     7741 gccttggaac atatgggagt gatctttaac cgcaatgaag aaggccacct cggtacgcgc
     7801 cgattcggtg gacagaaacg cgcacggacc tttttcgtgt ccgactttac cgggcaggcc
     7861 atgcttcacg tgctgtatga acaaaccatg caggctaaaa ttcccgtcta cgaagaatgg
     7921 tttgtgacct ctctcgtgaa agacgaccag ggacggtgtg cgggagtggt ggcctttgaa
     7981 attcgcaccg gaacattcgc actatttcga gccagatccg tgatcatggc cacaggcggc
     8041 ttaggccgtg tgtacgaacc cagcaccaat gccttgattt gtacgggcga tggcatggcc
     8101 gtggcctacc gggctggcgc ccccttaatg gatatggaaa tggtgcaata tcacccgacg
     8161 acgctgaagg ggaagggcct cttgattacg gaagccgcca ggggagaagg ggcctacctt
     8221 ctcaacagca agggcgagcg tttcatggaa cgttatgcgc ccaatatgct ggaactggct
     8281 tcgcgggatg tcgtctcccg tgcggaacaa attgaaatta atgaagggcg tggcatcaaa
     8341 ggttgcgtcc tactcgactg ccggcacttg ggacgagcct tccttcagga tcgtctcgga
     8401 caaatctacg ccgaagccaa aacctttgcc aatatcgatt tagccgaaga gccgttaccc
     8461 atccggccag gcatgcatta ccaaatgggg ggcatcaaaa ccgacaccga aggccgatgt
     8521 tgggatgtcc aaggccaatg gaccggcgtg ccaggcctct tcgcggcagg agaaaccgcc
     8581 tgcgtgagcc tgcatggggg caaccggctt ggcgcgaact ctctgttgga caccgtggta
     8641 tttggccgac gggcaggaac atgtgccgca gagtatgcca atacgaatcc tcctcccacc
     8701 attccggata gcgccatgga caaagacaaa catctggtgg ctaacatcgt gtcacggaaa
     8761 ggaccggggg aacgagcggc ggcgttgcgc ctggagatgg gcaccaccat gaaccggcat
     8821 gtatcggtct ttcgtgagga ggaaggcatg gagacggccc ttcaccatat caccacactc
     8881 aaaacacgtt ggccagacat cactatccag gataagggac aggtgtttaa caccggatta
     8941 attgccgcac tggaactggg gtttatgttg gactgtgcag aagccattat cgtgggcgcc
     9001 ctcaccagac aggaaagccg tggagcccat ttccggacgg attttcttga ccgggatgac
     9061 gaacactggc tcaaacatgt gttgctgtat cataggccag acagtcctcc ccatgtcgag
     9121 tatctaccag tgcggatcac ccaatggcaa cctcaaatcc gcgtctatta atggggacaa
     9181 aaatccgatc atgtcttttt tttcaaccgc gcacgaacga caagtcaccc cctaacggct
     9241 taaaggagat atactgtaag cacaagccga ggaattaccc acactgatgc acaccactct
     9301 tcgcatacgt cgctttaatc cggaacacga tcatccctcc tcgtactatc aggaatatga
     9361 tctggaaatc gatccttccg acagtgtcct cgatggactc attaaaattc gggaaaccat
     9421 tgatgattcc ctaacacttc gttgttcctg tcgcggggcc atttgtggat cttgcggtat
     9481 gcgcattaac ggacgcgcca cactggcctg taaaaccaaa atgatttcca tggcctcgga
     9541 aggtgaaacg attcaggttg aacccatgaa taatatgccg gtcattaaag atctggtcac
     9601 cgacatggcg cctttctggg caaagattcg gcagatacaa ccgtggctcc aaccgcaagg
     9661 gctgcctccc acggccgaat acaccgcctc accagagtcc atgagtcacc tctcaggtgt
     9721 catgagctgt atcatgtgtg gcgtctgcgt ctctgactgt actgtcctgg aggtcgacaa
     9781 gactttcatc gggcctgccg ccctggcgaa agcctatcgg ttcgtcgccg acccacggga
     9841 tgccgcgact tctcaacgcc tgaacttcct caaccaaccc agtggcatgt gggattgcac
     9901 ccgatgtatg gaatgtattc aggtctgccc caaaggggtc ggaccgatgg accggatcat
     9961 ggcgttgcgg gcaaaaggcc tggcgaaaaa agttccatca acctgcggtt cccgccatgc
    10021 cgaagtcttt accgatatta ttaaacataa aggcattctg gacgaaccca tgctggcgat
    10081 cagaaccttc ggccttaaaa atattggcag attactcgcc atgattccta tggtcctcca
    10141 atccctactc aaaggcaaag tccccccatt aggtccgctt caccgtccga tcccaggcat
    10201 ccagcacatt caacggctct ttaaacaggt caggattaca cgatgaaata cgcctttttt
    10261 cccggttgtg tctcaaaggg ggcctgcccc gagctgtatc aatccgtcat gcaggtctat
    10321 cccagattgg gaatcgaact cgcagaaatg acaactgcct cctgtaccgg tgcaggtgtg
    10381 ttgcaggaaa aagacccgcg gctaggcgat gcgctgaacg cccgtacctt tgcgctggcg
    10441 gaacaacaag gcctccccat catgacgatt tgtagtacct gccagggggt gatgagccaa
    10501 gccaaccatc gtctcaccac tcgaccacac tatctccagg aaatgaacga cttgctccgg
    10561 gaagaaggat taacctatcg gggaacaacc acaatcaaac atttcctgtg gattctcttg
    10621 gaagacgtgg gcgaggagac gttgcggaaa catatcaccc gtcccttatc cggtcttcgt
    10681 gccgcgccgt tttatggatg ctatatccaa cgaccgaccg atgcgctcga attcgaccgg
    10741 aatcctgatc ggggaaaatc tcttgaacgg atcatcgaaa ttttaggggc ggaagttgta
    10801 gactttccgg ggaaaacccg atgctgtggg tttcccatat tgaccattaa tgaaaaaaac
    10861 tcgctcacca tggtagccac ccatacgcgg gatgctaaat ctcacggagc ggatatcatg
    10921 gtcactccct gcccgctctg ccatctcaat ctcgacggca tgcagaccaa tgccgcccgt
    10981 caggaacaga cttccattga tcttccgatc ctccatcttc cacaactgct gggcctcgcc
    11041 atggggttgg atactaagag cctcgggtta caacgaaacc tcatttcccc tgaatctgtt
    11101 ctcgcaaaat tgagtatcta accctagttt tctttcgatt gacagagact aatattcctt
    11161 ctaccctcca tgttccatag actgggaaaa caccagaaat atacaaggag caagagaaca
    11221 tgagtacaaa gaccaaggac attttaaatc aagctctcgg gcttcctccg gttgaaaaag
    11281 cccaccttgt tgactgtctt cttgcaagtc ttgataagcc cgatcaagct attgataacc
    11341 tttggcgaga agaagtcgaa aaaagagttg aagcttacca atcagggaag atcctctctg
    11401 tatcccttca gcaagcttta tccaaatacc agagataatg gaaattcgac tattagaaat
    11461 cgccgaaaca gagcttgatg aggccgtcac attttacaat tcagaacaac ccgaattagg
    11521 tgcggatttt attgttgaat ttattcactc ccttgagcga atcaaaacct accccaaagc
    11581 ctggaaccct ttcactcaaa atacccgccg gtgtcaatta cgacgattcc cctatggaat
    11641 tgtctatcaa atactcgaag gcgaaatctt agttgtcgcc atttcccacc tgcatcgaga
    11701 gcctggatac tggaaagata gaataacgcc ttaggtcttg aaagcacgga attctctaag
    11761 caggattacc tctcctcatt gccaagactt aagccgatcg cataaatgcc ggttttcggc
    11821 ccggcagccg aactactttt gattcggcaa aagtagtcaa aaccagtgac gcccagtccg
    11881 gccacattgg aaaggacgaa cgcagggagg agagcggtcc aactcgctac gctcagacaa
    11941 ggcccgctag atatataaga gcgttcatcc cgagagccgg acagcaggcg tcggtcgtat
    12001 ggaaggagtg aagtgcttag ggctctagca gaggagccta gaatttaatc aagctgatgg
    12061 acaaatgaga tgacaaaaaa tgctaaaaat gtaacatatg gcccattctg ttacatgggt
    12121 gaaaagttct aaacgaattt ctcgatggct tgacccaggc tccagaacct ccgagcaata
    12181 ttcgttttct tgcctatcgc agggaaaaca aaaattgaaa ctgaaaaaaa aggtgtcctt
    12241 ttaagcttac aaggcgtccg ctctcggttg cggtttcaac cggtcgctgc aacatctaat
    12301 ctctaataat agagaggagg at
//
LOCUS       DAORAQ010000071        11982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_881_length_11982_cov_61.971996, whole genome shotgun sequence.
ACCESSION   DAORAQ010000071 DAORAQ010000000
VERSION     DAORAQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11982
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_881_length_11982_cov_61.971996"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..996
                     /locus_tag="PKM72_08075"
     CDS             358..996
                     /locus_tag="PKM72_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60780.1"
                     /translation="MIRLIEARQLKRSMLDILQVLLLVGILTGCGAMNAIPPPASPTA
                     VQQFLMAQAVERSLHGENTMPIPLASGDTVSLDISGLSVEQGLAVAQEFFKGAVGGWL
                     GEGGLKIVRDPAKAKYRIHILVQAFGTEQRSSLFGLPAIQSTLVPIALPEIAIYASQK
                     VSGYTRFRLDIYESATGQFTRSTPWFQGSTYFNEYTVLFFFDFEFTDLIAPF"
     gene            complement(1145..2761)
                     /locus_tag="PKM72_08080"
     CDS             complement(1145..2761)
                     /locus_tag="PKM72_08080"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNP60781.1"
                     /translation="MAYSEEGVRINYLMDFWGHSGNFPIADSIPGMQYRHLELVMGMK
                     MGNKKRKVRGHWFRQVFSPALAMWIGCAGVFAMSIGMEGALGWAGEPSESAIERTEER
                     TVKQGGFEDVAQAVTPSVVNITATIDIVDSRGGGVPLPFRGFGEGQPPPGIRKRQKGS
                     GSGVILSSDGYIVTNNHVIAESRDLLVTLPDAREFSAKVVGADPETDLAVIKIQAENL
                     PVIPWGNSSSLAVGQYVLAVGNPFGLNSTVTLGIVSALDRGGMGLARFEDFIQTDAAI
                     NPGNSGGALVNTRGELVGINTAIASQNGGYQGVGFAVPSSLARPVLDELITNGKIVRG
                     YLGLGVQEMTMELAPWFGLKNSEGALVTDVVPGGPADKAGIHRGEVILQYQGKHVKDG
                     QMLLEGVTRANIGEQIELGIMENGKERKVRIIIREQPPIPQVRRVRTPRVEHPLAGVE
                     VADVDEAAIQEYELSPATKGAVVSFLEEGCAAELAGIIEGDVIIELNKHPVESVREYS
                     SWYKRLKNEKVVLIVLVRASRHLYVLVKSS"
     gene            complement(2926..3693)
                     /locus_tag="PKM72_08085"
     CDS             complement(2926..3693)
                     /locus_tag="PKM72_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP60782.1"
                     /translation="MESSKTSKTDRVGTLDQDISRIRKQLEIIKRFLIDEPSPASLNE
                     FDSTTEDILADAFGSSSPLLDTYDYAQLGEAAGLMNLPEGAPEGTTHQSQRETVRQRQ
                     RVLESGIADLEARRASLSQESRKRKGSGPNVADYMAKTVRSVSLDATLQEVGQCLQKW
                     KIGSVLVQNGDEFLGYITETELTREVVASGTDPVTTTVKTCMREPLVTIETSDPIVEA
                     VRLMKEKATRHLAVTESNRIVGVVSVSDIIRYYSGVK"
     gene            complement(3866..5200)
                     /gene="mgtE"
                     /locus_tag="PKM72_08090"
     CDS             complement(3866..5200)
                     /gene="mgtE"
                     /locus_tag="PKM72_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNP60783.1"
                     /translation="MRDLTTQSGTKVRGRGHLLNEAFFQNHHEEAARILEGYPVEDIL
                     RVLQGTSPKNAANLLACITPPVAADTLAIMPTDLLKQVVPHLSPSLAASLFQRLDDDL
                     QRAILFRVPERYAQEIRSYMTYPEESVGRIMDPKFFVLQEESTVREAMVQVRIRAQKD
                     VHDVYVIDRTQKLVGRISVRDLLLVDPEEKLQSVMVQDLPTIHPLESREEIVELFGER
                     HFFTIPVVDLDKRLLGVVRNEAIIKASQEDASADLLTMVGANKDERALSPVWFSVRKR
                     LPWLQLNLLTAFAAAFVVGMFEGTIAKFTALAVLLPVVAGQSGNTGAQSLAVVIRGLA
                     LRDIRPSQWLRVSGKEVYVAFLNGLAVSATACFAVGLWSRSLGMVFIIGLSMIISMVI
                     AGLSGAIIPIALKALKQDPAQSSSIVLTTITDVVGFFSFLGLATLLSSLLET"
     gene            complement(5197..6033)
                     /locus_tag="PKM72_08095"
     CDS             complement(5197..6033)
                     /locus_tag="PKM72_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP60784.1"
                     /translation="MELEHRLTLGYLQAHPVEAARHLESLDPHESASLLEALPGEEIA
                     GVLEHCLPVSTAKIIEELSKDLGAGVLSAMNATSAIGVLRQFEEPVRQDILDRLDNPM
                     GATLRRALRYSPNTAGSLADPQVFTLPPDIAVEEAIDRIRTYGQKAMYYVYVIDRDSK
                     LEGVLTLRQLLIDRSDHLVGTLMETQITTLSAEANLEEILKHSEWSRFHTLPVVDRWG
                     TFFGALRYRMLRRIEKDVGGKAPLGSVSDSLMQLWEVYSLTGIRLMTDVAETLQERNK
                     MG"
     gene            complement(6037..6885)
                     /locus_tag="PKM72_08100"
     CDS             complement(6037..6885)
                     /locus_tag="PKM72_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNP60785.1"
                     /translation="MQEKKLTMAGWVDGLAEAVTASLAQIVAYLPTLILACMLLGLGY
                     VLARLVSVVVTRLLQLMGFDRLLSRTAVQTLLERSGTKQKSSEILGMIGFWIIFLVFL
                     IQASDTLSLTMVSDALTSIAYYIPKVGIAVLVLVLGLIAANFVRELITMTCSSAGITH
                     GTMVAQAVYVAVVLLIVVTAIDALGINTELLNNTIVILLAGLIGGAALSFGLGSRTAV
                     ANLIAAHYLGSMVRVGMTVKIGENQGTVVAVTPISVVLETRDGRVIVPATQVTESTAV
                     ITHTEH"
     gene            complement(7089..9323)
                     /gene="malQ"
                     /locus_tag="PKM72_08105"
     CDS             complement(7089..9323)
                     /gene="malQ"
                     /locus_tag="PKM72_08105"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNP60786.1"
                     /translation="MNNHEFATKPIWQLGQQYGIGSVYHDGLGVERQVTVPHLRKVLE
                     SMGVKASSKQEIRDSLSHAESQSWTQVIDPVVVRYPSDSPFELPLSLPLDDISLEKVS
                     LTVQVTDEKGGCRHLTLRGAGGEVKAEKAIQGVKYVQVHFPLSGKFPLGYFRLSVRVK
                     FDSKRTIEAHTLLIVAPRKCYLPSSPKRPWGISLQLYGLRSRKNWGIGDFRDLGDVMK
                     VAGARWGVATIGVNPLHIPASGLTSPYSPSSRLFWSPMYLNLEGVDEWRTSTGLRQKA
                     KGAKFQNTLQQLRESPLVDYAAVGKLKWTVLEELFRVFRRHHLQPKTLTARGRAFTRF
                     VSGFHPHLTMFCTFQVLTEHLETVAWRTWPKAFQHPQSPDVEVFQRSYATRVQFYQYV
                     QWLCELQLQQLDHVAKKHSLSLRLYHDLPVGIHPDGADAWVFQDQLASDITVGAPPDS
                     FNLNGQSWGLVVPNPVRLREDGYRFFRETLRQNMRHGGVLRIDHALGLFRMFWVPQGG
                     TGKDGVYVRTYVDEILAILALESVRRKVMVVGEDLGAVTPAIREKLDAAGLLSYRLLF
                     FERENGGAMIPPHAYPEQALVAATTHDLPTLKGFWAGQDILIRQEAGVYLRKMDIEQD
                     QRGRAADRKRLWEALHREGLCSAEAMPSSLSVDMIQVLYRYLARTPSRLLIVQLEDLL
                     AEIDTPNLPGAPDSVYPSWRVRIGRDLTAWLKDPAILRFARAMQRERRKGGGTHRMSR
                     ETVS"
     gene            9735..10067
                     /locus_tag="PKM72_08110"
     CDS             9735..10067
                     /locus_tag="PKM72_08110"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNP60787.1"
                     /translation="MPPKTPDEKPVKKKSSTTKKTPTAGKTASKTSGKSVASATTKGP
                     AMSQTKHQNPQTSYPGPSMALSEELRERIAQRAYEIHLRRGGQHGSDWEDWLQAEREI
                     LLLQTPPV"
     gene            10145..10372
                     /locus_tag="PKM72_08115"
     CDS             10145..10372
                     /locus_tag="PKM72_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNP60788.1"
                     /translation="MDNDTRYVLLAGLSLMVGVVLGTGLGLLMAPQSGSRTRRQLKNM
                     MEDASERAGEFADDAKEAVTGIVERSKKFVV"
     gene            complement(10526..>11982)
                     /gene="glgX"
                     /locus_tag="PKM72_08120"
     CDS             complement(10526..>11982)
                     /gene="glgX"
                     /locus_tag="PKM72_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNP60789.1"
                     /translation="WGYNSIGYFAPDIRYSAFPGGGEKVDEFKSMVRKLHSAGIEVIL
                     DVVYNHTGEGNHFGPTLSFRGIDNASYYRVSPDNPRYYMDYTGCGNTLNVRHPRTLQL
                     IMDSLRYWVIDMHVDGFRFDLASALARELHDVDRLSAFFDIIHQDPVLSQVKLIAEPW
                     DLGEGGYQVGNFPPGWTEWNGKYRDSIRRYWKGDGGLLGEVANRWSGSSDLYGESGRQ
                     PYASINFVTAHDGFTLQDLVSYDHKHNEDNQEGNRDGTDDNDSWNCGVEGPTDDPAIQ
                     ELRDRQVRNFFATLLLSQGVPMICGGDELGRTQQGNNNAYCQDNELSWVNWNLTRSQL
                     GLLDFVKLLIDIKKTHPVFQRRRFFQGRRVEDSEVKDIAWFGSHGKEMSHEDWQMGLR
                     TLGIRLAGDAIVEKDSQGRPIVDETFLVLLNAHHEDVDFILPAHTKSVRWELEFDTAV
                     PVDKKSPKHTKLLKGGQPYHLISRGLALFRTPKS"
BASE COUNT         3156 a         3313 c         2674 g         2839 t
ORIGIN      
        1 taccacccaa gacggaaccg ttttagcaac ctttcaacaa caggttcagg aaattgacct
       61 cctcattgaa gaattgacct cctctttgaa gagcatgatt tggtaacatt ggacgtgagg
      121 caatacgaaa tttttgtgaa caaccccccg ttgcttcgtg tcaccaaatt tacggacttg
      181 ccccctgcta gtgacgctaa cccgagttcc ttcattcaat ctgactctac atggaaaatc
      241 ggctcaacac atgatcccac aggatttctc ctttcgacta aggaggcata tcgaataccg
      301 gttttccttc aagttgactc acgtattttt ctgctcctat caaaaaatga ggtgaaagtg
      361 attaggttaa tcgaagcacg tcagttgaaa aggtccatgc tcgatattct acaagttctc
      421 ttgttagtgg gaatactgac cggctgcggc gccatgaacg caattccacc acccgcatcg
      481 ccaacggcag tccaacaatt tctcatggct caggctgtgg agcgcagcct gcatggagag
      541 aacaccatgc ccattccctt agcctctgga gatacagtaa gtctcgatat ctctggattg
      601 agcgttgaac aaggattggc tgttgcccaa gaatttttca agggtgcggt tgggggttgg
      661 ttgggagaag gagggttgaa aatcgtcagg gatccagcta aagcaaagta ccggatccac
      721 attcttgtcc aggcttttgg aacagaacaa cggtcctcct tgttcgggtt acctgcaata
      781 cagagtactc ttgtcccgat tgccctgccc gagatagcga tatacgcttc tcaaaaagtg
      841 tcaggatata cccgattccg tctggatatt tacgaatcgg caacgggaca atttactcgc
      901 tctacgccat ggttccaagg gtcaacctac ttcaacgaat ataccgtctt gttttttttc
      961 gacttcgagt ttaccgactt gatcgctcca ttttgatctc ttgccctaat tttcaaccgg
     1021 gacccttcaa acatctaagg ttgcttgaaa aatcaggacg catcgctcaa aacgtgtaat
     1081 cgtcaaacta aagctgtggt aaagaatttg tagaacgccg caaacccgac tcacactggc
     1141 tccctcagga ggatttcacc agcacataca aatgacggga ggctcgaaca agcacaatca
     1201 gtacaacctt ttcgttcttc aaccgtttat accaggagga atactctctg acggattcca
     1261 cgggatgctt attcaattca ataatcacat cgccttctat gatcccagcc aattcagcag
     1321 cacacccttc ttcgaggaat gagaccacag ctcccttggt tgcaggactc aactcatact
     1381 cctgaatggc cgcctcatcc acatcggcca cttccacacc ggcaagcgga tgctccaccc
     1441 gaggcgttcg aacacgccgc acctgaggga tcggtggttg ctcacggatg attattctta
     1501 ccttacgctc ttttccattc tccataatac ccaattcgat ctgttctcca atattggcac
     1561 gtgtcactcc ctccaatagc atctgtccat ctttcacgtg tttcccctgg tactggagaa
     1621 tcacctctcc ccggtgtatg ccggctttat ctgccggtcc accaggaacc acatcggtta
     1681 ctagagcacc ttcactgttt ttcaggccaa accacggagc caactccatc gtcatttcct
     1741 gcacccccag acctaaatac cctcggacaa tctttccatt agtaatcaat tcgtctaaca
     1801 caggtcttgc caatgaagaa ggcacagcga accccacccc ctgataccct ccattttggg
     1861 aggctattgc cgtattaata ccaactaatt ctccccgtgt gttcaccaac gctccacccg
     1921 aatttccagg gttaattgcc gcatcggtct gaatgaaatc ttcaaagcgg gctaacccca
     1981 tgcctcctcg atcaagagcg ctgacgatac caagcgtcac cgttgaattc agaccaaacg
     2041 ggttgccaac agccagtaca tattggccaa cagctaacga ggaggaattt ccccatggaa
     2101 tcactggaag gttttcagcc tgaattttaa taaccgccaa atctgtttcc ggatccgcac
     2161 caaccacctt ggctgaaaat tcacgcgcat ctggaagagt gacaaggaga tcacggcttt
     2221 cggcaattac atgattattg gtcacaatat atccgtcgga agacaagatg actcctgacc
     2281 ctgaaccctt ttgacgtttg cgaatacccg gtggcggttg gccctcacca aaacctctaa
     2341 acgggagtgg cacgcctcca ccccgagaat caacaatatc aatggttgcg gtgatattga
     2401 ccacagacgg ggtcaccgcc tgagccacgt cttcgaatcc tccttgcttt acggtccttt
     2461 cctccgttcg ttctatggcc gactctgatg gttcgcccgc ccaccccaga gctccctcca
     2521 ttccgatact catggcaaaa acaccggcac aaccgatcca catcgccaag gccggactaa
     2581 agacctgtcg gaaccaatga cccctcacct tcctcttttt attgcccatt ttcatcccca
     2641 tgactagttc tagatgcctg tactgcattc ccggaataga atctgcgatg gggaaattcc
     2701 ccgaatgtcc ccaaaaatcc atcaggtaat ttattcttac accctcttca ctgtatgcca
     2761 tcccatcctc aagccttttt tatatgagcg gatcgaagat tttttcaaat gagggtgacg
     2821 ccctcctcaa ggaacacgac ggcaaaaaag catgtgtttc cccgaatagg agagcatcgc
     2881 aaccagaggt tagaaggcga gggagagtct ctactcaagc aacgattatt taaccccgga
     2941 ataataccgg ataatatccg aaacggagac gaccccgacg atccggttac tttctgtgac
     3001 cgcaagatgg cgtgtcgctt tttctttcat caggcgcaca gcttccacaa ttggatcact
     3061 ggtctcaatg gtgactaacg gttcacgcat gcaggtcttc accgtagttg tcacggggtc
     3121 tgttccggat gcaacaacct ctcgagttaa ttctgtttca gtgatatatc ccaagaactc
     3181 atccccattt tgaaccaata cggaaccgat tttccatttt tgcaagcatt gtccgacttc
     3241 ctgcaaggtg gcatcaagcg atacagatcg aacagttttt gccatatagt ccgcaacgtt
     3301 tggaccggaa cctttccgtt tcctggattc ctgtgagaga gaggccctcc tggcctcaag
     3361 atcggcaata ccgctttcca gaactcgctg acgttgccgg accgtttcgc gttgggattg
     3421 atgggtggtg ccttccggcg ctccttctgg aagattcatg agccccgctg cctccccaag
     3481 ttgggcatag tcatacgtat ctaatagcgg ggaagaactt ccgaatgcat cggcgagaat
     3541 gtcttcggtg gtcgaatcaa attcgttcaa ggatgcggga gaaggctcat ctatcagaaa
     3601 tctttttatg atctctaact gcttacgaat tcgagaaata tcttgatcca atgttcccac
     3661 tcgatccgtt ttagacgtct tggatgattc cataaagcct cctctcctca atgattaaga
     3721 taaaaatgag cgaaaaaacg atactaccct accattaatg agtaggcaat cagaagtttt
     3781 cctggaatga aaagcccctc ccgaggcgga atgggagggg aatggagcag agatgaagcg
     3841 gacaccgccc ccaaccgatc aatgatcaag tttccaacaa acttgaaagc aaggtggcaa
     3901 gccctaagaa actaaaaaac ccgaccacat cggtgatcgt cgttaacaca atggaggagg
     3961 attgcgccgg atcctgtttg agtgccttca aagcgatggg aatgatcgcg ccggataacc
     4021 cggcaatgac catcgaaata atcatggaca atccgatgat gaagaccatg cccagcgacc
     4081 ggctccataa gcccacggcg aaacaggctg tggcagagac ggccagccca tttaaaaatg
     4141 cgacatagac ttcttttcca ctgacccgga gccattggga tggtcggata tcccgtaaag
     4201 ccaacccgcg aatgactaca gcgagagatt gagccccggt attgcctgat tgcccggcga
     4261 caaccggaag caggacggcc agggccgtaa atttggcaat cgttccttcg aacattccca
     4321 ccacaaaggc agccgcaaat gccgttaaca ggttgagctg caaccacggc aatcgttttc
     4381 ggacagaaaa ccatacaggg gaaagcgcac gctcatcctt attcgccccc accatcgtga
     4441 gtaggtccgc actcgcatct tcctgactgg ctttaataat ggcttcgttt cggacaacgc
     4501 ctaacagccg tttgtccaaa tccacaacgg gaatcgtaaa aaaatgacgt tcaccaaaga
     4561 gttcgacaat ctcttcacgt gactccaaag gatgaatggt aggaagatcc tgaaccatga
     4621 ccgactgcaa tttttcttcg ggatccacca gcagcaagtc acgaacggaa atccggccca
     4681 caagcttttg agttcgatca atgacataga catcgtgaac atccttttgc gccctgatcc
     4741 gcacctggac catggcttcc cgaaccgtgc tttcctcctg taacacaaaa aattttggat
     4801 ccataatacg accgacggat tcctccgggt aagtcatata ggaccggatt tcctgcgcat
     4861 accgttcagg gactcggaaa agaatcgccc gttgcaaatc gtcgtccaat cgttgaaaca
     4921 aggaggcagc caatgacggc gaaagatgcg gcacaacctg cttcaggaga tcggtgggca
     4981 tgatcgccaa cgtatccgcg gcgacgggag gagtgataca ggctagtaga ttcgcggcat
     5041 ttttcgggct ggttccttgc aagactcgca gaatatcctc caccggatac ccttccagaa
     5101 tacgtgcggc ttcctcatga tggttctgaa aaaatgcctc gttgagaaga tgacctcttc
     5161 cccgaacctt tgtgccgctc tgcgtggtca gatccctcac cccattttgt tccgttcttg
     5221 cagggtttcc gcgacatcgg tcattaaacg aatgccggtt aacgaataca cttcccaaag
     5281 ttgcatgagc gaatcactca ccgaacccaa tggggccttg cctccgacat ctttctcaat
     5341 cctgcgtaac attcgatatc gcaaggcgcc aaaaaaggtt ccccatcgat ccaccaccgg
     5401 caatgtatga aaccggctcc actcagaatg tttgagaatt tcttccagat tggcttctgc
     5461 tgataacgtg gtgatctggg tctccattaa tgttccgact aaatgatcag accgatcaat
     5521 gagcagttgc ctcaatgtga ggacaccttc caatttgcta tcgcgatcga tgacatagac
     5581 atagtacatg gctttctggc cataggtacg aatacggtct atagcctcct cgacggcaat
     5641 atcaggtgga agcgtgaaca cctgaggatc cgctaagctt ccggccgtgt tgggtgaata
     5701 ccgcaaggca cggcgaaggg tagcccccat cggattatcc aaccggtcta atatgtcctg
     5761 gcgaacaggt tcctcaaatt gccgaagaac cccgatagca gatgtggcat tcatggcgct
     5821 taagacaccc gctccgagat ctttcgaaag ctcttcgatg attttggcag tggacaccgg
     5881 caagcaatgc tctaacacac cagcgatttc ttcgccgggc aaggcctcca ggagcgaagc
     5941 cgattcatga ggatcgaggg attccaaatg acgagccgct tccacgggat gggcctgcag
     6001 atacccaaga gtcagccgat gttcgagttc cataggctaa tgctcagtat gggtgatgac
     6061 ggctgttgat tcggtgacct gggtagcagg aacaatgact cgtccatccc tggtttctaa
     6121 cacgaccgag atgggtgtca ccgcaaccac ggttccctgg ttctccccga tttttacggt
     6181 catgcccacc cgaaccattg atcccaaata atgagcagca atgagattgg ccacggcagt
     6241 tcgtgacccc aacccaaacg acaacgctgc acctccgatc aatcccgcca ataagataac
     6301 aatggtattg ttcaataatt ctgtattaat cccgagtgca tcaatggccg tcaccacgat
     6361 caacaggacc acggccacat agaccgcctg ggccaccatc gtcccgtggg taatccctgc
     6421 cgagctacac gtcatggtaa tcaattcacg gacaaagttc gcggcgatca aacccaaaac
     6481 cagcaccaac acggcaatac cgactttggg aatatagtag gcaatactcg tcaacgcatc
     6541 cgagaccatc gttaaactca gagtgtcaga cgcttgaatg agaaagacca ggaaaataat
     6601 ccaaaaacca atcatcccta atatttcgga acttttctgc ttggttccag atcgttccaa
     6661 caaggtttgc acggcggttc gactcagcaa ccggtcaaac cccatcaatt gcagcaagcg
     6721 ggtcaccacg acagacacca gcctggccag cacatagccg agacccaaca acatacaggc
     6781 caaaatcaaa gtgggtagat aggcaactat ttgggccagg ctcgcagtaa ccgcctcggc
     6841 taagccatcc acccatccgg ccatcgtcag ttttttctct tgcatatgaa ggatcgccct
     6901 ctcttcctga tcgccctact tagaggaaat accgtgtttt cccaaccaga ttcgatggtc
     6961 atgggaatat ttgacaatcg ttgatcatag tcccctctta gacatcacgc aactgacatt
     7021 cgggcgagat accccatttg attcaattgg gtatacaacc tgactggcca tccttaaaag
     7081 ggaagagttc atgaaaccgt ttccctggac atccgatgcg tacctccacc cttccttcgc
     7141 tcgcgttgca tagcccgagc aaaacgcagg atggccggat ctttcagcca ggcagtcaag
     7201 tctcgaccaa tccgaacccg ccacgaagga tagactgagt ccggtgctcc aggcagatta
     7261 ggtgtatcaa tttctgccaa cagatcttca agctgcacga taagcaggcg ggaaggtgtc
     7321 ctggctaaat accggtacag gacttgaatc atgtcgaccg aaaggctaga gggcatcgcc
     7381 tcggcagaac agaggccctc acgatgtaaa gcctcccata accgtttccg atcagcagca
     7441 cgccctcgct gatcttgctc gatatccatt ttcctgaggt atacacccgc ttcctgccta
     7501 atgaggatat cttgtccagc ccaaaaacct ttaagggtag ggaggtcatg tgtcgtggcg
     7561 gccacaagcg cctgctcagg ataggcatgt ggcgggatca tagctcctcc gttctctcgt
     7621 tcaaaaaata gcaggcgata ggatagaagt ccggccgcat ctaatttttc tcgaatggca
     7681 ggagtgaccg caccaagatc ttccccaacc accatcacct ttcgtcgcac actttctaaa
     7741 gccaggatcg ccaaaatttc atccacatag gttctgacat agacgccatc cttacctgtt
     7801 ccgccttggg gaacccagaa catccgaaac aagcccagag catggtcgat ccggaggact
     7861 cctccatgtc gcatgttttg ccgtaaggtt tccctaaaaa atcgataacc gtcttcccgc
     7921 aatcgaacgg gattgggaac gaccagtccc caactttgtc cattgagatt aaaggagtcc
     7981 ggcggcgcac cgacggtgat gtccgatgca agctgatctt gaaagaccca ggcgtcggct
     8041 ccatccggat gaatgccaac cggtagatcg tgatacagcc ggagagacaa cgaatgtttc
     8101 ttcgcgacat gatcaagttg ctgtaattgc aactcacaca gccattgaac atattgataa
     8161 aattgtacgc gagtggcata ggatcgttga aaaacttcca catcaggaga ctggggatgc
     8221 tgaaaggctt tcggccaggt acgccaagcc accgtttcaa gatgttcagt caagacctga
     8281 aacgtgcaaa acattgtcag atgcgggtgg aagccggaca caaatctggt aaacgccctc
     8341 ccacgtgccg tgagggtttt aggctggaga tgatgccgtc gaaagactcg aaataattcc
     8401 tcaaggactg tccatttcaa tttccctacg gctgcataat cgaccagtgg actctcccga
     8461 agctgttgga gggtattctg aaattttgca cccttggctt tttggcgaag accagtcgaa
     8521 gtccgccatt cgtccacgcc ttccagattc aaatacatgg gactccagaa aaggcggctg
     8581 gaaggggaat aggggcttgt taatcctgac gccgggatat gcagaggatt aaccccgatg
     8641 gtggccaccc cccaacgagc ccctgccact ttcatgacgt cccctaaatc tcgaaaatct
     8701 ccaatccccc aattctttcg ggatcgtaag ccatagagtt gcagagaaat tccccaaggc
     8761 cgcttaggcg aagatggaag ataacatttc ctgggtgcca caatgagtag cgtgtgggcc
     8821 tcaattgttc gtttagaatc aaacttcaca cggaccgaca gacggaagta tccgagcgga
     8881 aattttcccg aaagagggaa atgcacctgg acatatttaa ctccttggat tgccttctca
     8941 gctttcacct caccaccggc acctcgcagt gtcaggtgcc gacagccccc cttctcatcc
     9001 gtcacctgga cagtgaggga taccttctcc aaactgatat catcaagagg aagagacaga
     9061 ggtaattcga acggggaatc agaaggatac cgcaccacca ctgggtcaat aacctgtgtc
     9121 caggattgtg attccgcatg agaaagggag tcacgaattt cctgtttcga tgaggccttc
     9181 acccccatac tttccagtac tttcctgaga tgaggcaccg tgacctgtcg ctcaacacct
     9241 aatccatcat gataaacgga tccaatgcca tactgctgac ctaactgcca aatcggcttc
     9301 gtggcaaatt catggttatt catcgttggt actcaaaaaa atattttttg atggctatta
     9361 gtgagaaaat ctctctcaac cctttgagta tcttccattc acaaacctac gttttccttg
     9421 cgtttaacca ttgtaaggga agatccatct tgtgtcttct acaaacctct ttgttaacta
     9481 ccaaataaac agatcgcata aatcaaaatt ttgcgcgttc accactcacc tgggccttgg
     9541 catgggaaca tttccaccca ctcacaggaa aactcctttt cctcttttcg taactctgca
     9601 atggccatgc agaatgactc aatccagcca ggcagcttgg acgaagagcc ctatatccgg
     9661 tacaataacc tataatatga gtcatattgg ggttcctcta aattcagaca agtataggag
     9721 ctgagaaccg aatcatgccc cctaaaacac cagacgagaa accggtcaaa aagaaatcgt
     9781 caaccacaaa aaaaacgccc actgcaggca agacggcctc aaaaaccagc ggaaaatctg
     9841 tagcatcagc taccaccaaa ggcccagcga tgtctcagac taaacaccag aatccccaga
     9901 cctcctatcc aggtccctct atggctctat ccgaagaatt gcgggaacgg attgcccaac
     9961 gcgcctatga aattcacctc cggcgcggag gccaacatgg cagtgattgg gaggattggc
    10021 ttcaagccga gcgtgagatc ctcttactgc agactccacc agtttagtgg acgattggaa
    10081 tattgatttt tgacaaccac atacccatta ctcactgacc ttcaaacaat ggaggatatc
    10141 ttacatggat aacgacactc gatatgtttt actcgctgga ctttcattaa tggtcggagt
    10201 ggttctcgga acgggactgg gtctgttaat ggctccccag tcaggatcac gaacacgtcg
    10261 tcagttaaag aacatgatgg aagatgccag cgaacgggcc ggagaatttg cggatgatgc
    10321 caaggaagcg gtcactggca ttgttgagcg cagcaaaaaa tttgttgtct aacggattga
    10381 tgcaaaggta ctgtccccaa aacgctcatg tctccggaca tgacctaaaa ctacctgccc
    10441 agatgagggc atggtgggtg attcggttcg gcaccatcct tattcctcca tcggtcccag
    10501 tgatgggtgg caatggagac tttggtcagg atttcggagt tcggaacaag gcaagcccac
    10561 gagagattaa atggtagggt tggcctcctt tcagcaattt cgtatgctta ggacttttct
    10621 tgtcaacagg cacagccgtg tcaaattcca actcccaccg caccgacttg gtatgagcag
    10681 gaagaataaa atccacatcc tcatgatggg cgtttaataa tacgagaaac gtctcatcta
    10741 caatgggccg cccctgacta tctttctcca caatggcatc ccctgctaac cggatcccca
    10801 aggttcgcag ccccatttgc caatcttcat gagacatttc ttttccatgg gatccgaacc
    10861 aggcaatatc cttcacttct gagtcctcga cccggcgccc ttggaaaaac ctccggcgtt
    10921 gaaacaccgg atgggttttt tttatgtcaa tgagcagttt cacaaagtcc agcaatccga
    10981 gttgggatcg cgtcaggttc caattcaccc aacttaactc gttatcctgg caataggcat
    11041 tgttattgcc ctgctgtgtg cgacccaatt catctccacc acagatcatc ggcactccct
    11101 gagataacaa cagggtggcg aaaaaattcc ggacctgtcg atcacgtaat tcctgaatcg
    11161 cgggatcatc ggtcggacct tcaacgccac aattccaact atcattgtca tcggtcccat
    11221 cccgatttcc ctcctgattg tcttcattat gcttgtggtc ataagacact aaatcctgga
    11281 gggtaaatcc gtcatgggcc gttacaaaat tgatactggc atagggctgc cgcccgcttt
    11341 ctccataaag atcgctgctc ccggaccaac gattggcaac ttccccaagt aatccaccat
    11401 cccccttcca ataccgacgt atagaatcgc ggtactttcc attccactcc gtccaccctg
    11461 gtgggaaatt tcccacctga tagccgcctt ctcccagatc ccagggttcc gcgatcagct
    11521 tcacctgcga caacacggga tcctggtgaa tgatgtcaaa aaaggcactg agtcgatcca
    11581 catcatgtaa ctctcgtgcc agtgcggatg ccagatcaaa acggaaacca tccacatgca
    11641 tatcgatcac ccaataccgc aagctgtcca tgatcagctg taaggtccgt ggatgtcgga
    11701 cattcagcgt atttccgcaa ccggtgtaat ccatgtaata gcgtggatta tccggggaca
    11761 cgcgataata ggaggcgtta tcaatccccc ggaatgatag ggtcggtccg aaatgatttc
    11821 cttctcccgt atggttatag accacatcga ggatgacctc gatgccggca ctatgaagtt
    11881 ttctgaccat ggacttgaac tcgtcaacct tttctcctcc accgggaaaa gccgaatacc
    11941 ggatgtccgg ggcaaaatac ccaatggagt tgtaccccca at
//
LOCUS       DAORAQ010000072        11971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_882_length_11971_cov_65.867573, whole genome shotgun sequence.
ACCESSION   DAORAQ010000072 DAORAQ010000000
VERSION     DAORAQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_11971_cov_65.867573"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="PKM72_08125"
     CDS             <1..1478
                     /locus_tag="PKM72_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNP60790.1"
                     /translation="LGVTQIQCSQAPSSTPAEPPPSITAAPNGTDVPVAVVHAADSSP
                     VVKASTPLLASNETFVKIAKEAMASVVNISATKRTVQSQGTNPLLEDPFFRRFFGEEF
                     ERRFKQPRERQEQGLGSGVIVSDDGYIVTNNHVVEQADDLMVLLGDKRKLPATLIGTD
                     PKTDLAVIKIEATGLSTLPWGNSVALEVGELVLAVGNPFGLNQTVTMGIISAVGRANV
                     GIVDYENFIQTDAAINPGNSGGALVNLQGQLIGINTAIFSRTGGYMGIGFAIPSQMVK
                     SVMQSLIGHGKVIRGWLGVSIQELSQDLATQFDVPDTQGALVGDVFSESPAGQAGLKR
                     GDIIRTYNGVTVKDPNHLRTLVAETTPESSIPLEVWRNGKIVNLSVSITEMPKDTAGL
                     TGESPSSVSGNHALSGLSVEPVQPGQTRDGQGVVVTQIAPNSPAERAGVQPGDVILEL
                     NRSPIGSVRDFEQLVKRLETGTSVLVLLQRGKGAIFLTIKP"
     gene            1572..1940
                     /locus_tag="PKM72_08130"
     CDS             1572..1940
                     /locus_tag="PKM72_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNP60791.1"
                     /translation="MSQLLCFLVLLLFPVSTQPILWASSPQIDIQSAPPFFSPNHLMI
                     RLDTALTWKNNTGEAHSIVADDCQSRSRCSFDSGVIRPENQYVLTRLAPGRYPYHCGL
                     HPFMRGLLTVQTTASPSSDI"
     gene            2122..2199
                     /locus_tag="PKM72_08135"
     tRNA            2122..2199
                     /locus_tag="PKM72_08135"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2158..2160,aa:Glu,seq:ctc)
     gene            2318..3409
                     /locus_tag="PKM72_08140"
     CDS             2318..3409
                     /locus_tag="PKM72_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNP60792.1"
                     /translation="MTTRTFHDGESDGLEALEALDPEAIESFSDLLAAMKKTAFGGRR
                     LGDAFEILKKMVEDPDCTVILTLSGAMTIAKMGKIICSMIDRNMVQAVVSTGALIAHG
                     LSEAVGKTHYKYHPSMTDTELYEKGYNRVYDTLEMETNLNHVERVVSQTLKRMTPEIP
                     ISSEILTREIGKTLHDDYEGPGILKSAYEQGVPVYIPAFTDSEMGLDVSIWAMREKRQ
                     SQAQPPPDLSDQETWQLLQQSRPAFNPFLDLNSYTEKLLSAKRLGIFTIGGGVPRNWA
                     QQTPPYIEILNLRMGTQLTPPRYQYGVRICPEPDYWGGLSGCTYEEGVSWGKFVPRKE
                     GGMFAEVLSDATVVWPLLMMGLLESARRR"
     gene            3486..4136
                     /locus_tag="PKM72_08145"
     CDS             3486..4136
                     /locus_tag="PKM72_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNP60793.1"
                     /translation="MMNIGQSLKSTIAFAILLGTLLISPMTHGKTPDFLIQDDDVVRG
                     DPKAPITLLEYSDFTCGFCEKFFHETFPKLLSEYIETGKVRFVYRDFPRGMGSPLRAA
                     DAARCAGEQNAYWPMHDRLFNSDGQLAPDNLKQYAKDLNLHVEQFSECLASHRYLKDI
                     EKDLQDAGSLGIRGTPAFVLFPTTLPEDPHLILIPGAFPYETFKEEIDKLLTLHGA"
     gene            4309..4569
                     /locus_tag="PKM72_08150"
     CDS             4309..4569
                     /locus_tag="PKM72_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60794.1"
                     /translation="MTESQSFWPVECAQGEPDLFVCLTCFDEVFKAKMPVDGCPSCGA
                     IAAFEPFSLDAIKEWGTENLIQKAEHLPSSSDPGSDQPPSSI"
     gene            4602..6035
                     /locus_tag="PKM72_08155"
     CDS             4602..6035
                     /locus_tag="PKM72_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNP60795.1"
                     /translation="MTVATAKPILIGGQWVKTPSAEPVRNPYSNEVLAMVCQAEPEHI
                     HQVTNSAKQVSSECARIPAHARAKALLHIATGLSTRREEFASTICQEAGKPISDARRE
                     VDRAIQTFRLAAEESTRIPGEILPMDLTPGGEAYSGSVTRFPIGTVLGITPFNFPLNL
                     VAHKVAPCLAAGNAMVLKPAPQTPLTSLMLGEVFLTTELPPEMLTIIPCSNTLAEQMV
                     IHPNFQALSFTGSMTIGWMLKGKAGYKRVLLELGGNAGVIIEPDANLDVAIDRCVAGG
                     YGYAGQTCISVQRIFVHESRYDDFLKAFVDRVRLLPMGDPSLENIVVGPLISEQAARR
                     VEAWIQEAVSQGARVMTGGTRKDSFIEPAVLTHVSNNMKVCCEEIFGPVVTVTPYANL
                     EEAFALHNDSDFGLQTGIFTSNIDAMYQAYSRLEVGALLVNEIPTFRADHMPYGGIKQ
                     SGLGREGVRYAIQELTEPKLLLIKRSS"
     gene            6275..6865
                     /locus_tag="PKM72_08160"
     CDS             6275..6865
                     /locus_tag="PKM72_08160"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNP60796.1"
                     /translation="MVPTQMFLTRGVGVHKEKLTSFEEALRSAGIAYCNLVTVSSIFP
                     PDCKIIPRKRGEQLLNPGEITFCVMARSDTDERNRLISASIGLAIPSGRKVNYGYLSE
                     HHGYGETDEEAGEYTEDLAAQMLATTLGIKFDPNVAWKEREQVFKMGKDIVRTQNITQ
                     SAIGKPNLWTTVVACAVFIPLENITEDPKPRHKKSK"
     gene            complement(7020..7796)
                     /gene="xth"
                     /locus_tag="PKM72_08165"
     CDS             complement(7020..7796)
                     /gene="xth"
                     /locus_tag="PKM72_08165"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNP60797.1"
                     /translation="MKIATYNVNSIRKRIGILHKWLWQHNPDVMCLQETKVQDHEFPL
                     QAFADLPYHINFCGEKSYNGVAIFTRTPPELVSFGLDDGEPQEDPARLARVVINGITI
                     INTYIPQGFLIDSPKYAYKLEWFQRLRRYFSRVGSTGQPVIWCGDMNVAPEPIDVHSP
                     EKHLKHVCFHEDVRRAYQETVSWGFVDVFRHHFPHQQQFTFWDYRRPEALEANRGWRI
                     DHILVTPPLLSHSHTVKVDVQPRREESPSDHTVLWAEFSL"
     gene            7907..8527
                     /locus_tag="PKM72_08170"
     CDS             7907..8527
                     /locus_tag="PKM72_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60798.1"
                     /translation="MTTSHSFTPDELTLLADRDFFRKKAKVSLKIKQILEELQERLEA
                     EIAFQPLLAPEGFDSQARQFVKGEHLEDFPYQYVDFPRLYTRETKFAFRSLIWWGHHI
                     VFALMIEGPLIKQYRRNLFNRYLEVADRDLCLCLSPSLWEWKYGPGLTLPITRSRRSE
                     IAATLDHRTFFKIARFLPIDDSAVETGTIVDEGLKAFQAILPVIRT"
     gene            complement(8585..9130)
                     /locus_tag="PKM72_08175"
     CDS             complement(8585..9130)
                     /locus_tag="PKM72_08175"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNP60799.1"
                     /translation="MTDELMSQNSFSLILLAGFILGAVFLLRWIWRRPLPAGALPPLT
                     PESAESVVTARPLMSPGEATLYNLITLAARDHMLILAKIPLLSVLSVVDKDEEARKAV
                     MRTIQSVRCDVVLAHPGSLKVMTVVNLEKEVPSAVGREDRDRFVETLLKAAGIQSITL
                     QVTQSYSVDQLTELLGLAEEE"
     gene            complement(9170..10321)
                     /locus_tag="PKM72_08180"
     CDS             complement(9170..10321)
                     /locus_tag="PKM72_08180"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNP60800.1"
                     /translation="MGLMSGTSADGVDAAVVDIRGSGHRLKSSLLKHVGRSYSPRLRQ
                     LILQACEHGTVGQICHLNAVLGEVFAKTAMLAIKHSGISPQRVALIGSHGQTIHHRPA
                     AIYEPGIGQIRSTLQIGDPNVIAERTGVTTVSDFRARDLAVGGEGAPLAPYVHALLFA
                     QKRATRMVVNLGGIANVTVLPGGGNLAAVRAFDTGPCNMLLDGLVAMETQGQARFDRG
                     GRLALKGQVDKGLLRWLLAHPYMSRRPPKSTGREMFGEGYIQRVWAQAQRRHLAFSDL
                     LATSCRFIAQVIHDSQKWTKEIPDELVFGGGGVRNTRLWSELSGVFDPIPVRCMDECG
                     VSSQAFEAQAFAVLAYQTIHGVCANLPKVTGARHPVILGTVTPGIHGLP"
     gene            complement(10408..10839)
                     /locus_tag="PKM72_08185"
     CDS             complement(10408..10839)
                     /locus_tag="PKM72_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP60801.1"
                     /translation="MINFLDSHDIQAQQLLALFCQTDWACARSLDDTQRMLAHTDVAI
                     SAWDGPRLVGFGRVLTDFIYRASIWDVIVDRAYQDRDIGKGIIQRILRHPKLERVELF
                     WLCTRRYQGFYASLGFSDKEQTGMVWDRGKHIVPPTEPFNR"
     gene            10914..11093
                     /locus_tag="PKM72_08190"
     CDS             10914..11093
                     /locus_tag="PKM72_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60802.1"
                     /translation="MSMLVRKCPKCSKLFWKKEEEIQYADENTLKVVCNHCHQVLRIP
                     LITEGANAGAPKMGH"
     gene            11203..11928
                     /locus_tag="PKM72_08195"
     CDS             11203..11928
                     /locus_tag="PKM72_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60803.1"
                     /translation="MRHEHLAGLDVCITGGVDRQGSGDGPLVVLLHGFGAPGDDLVAL
                     WRYLNVPDEVRFLFPAAPLELGMGFGNARAWWMLDMERLTQARAQGQWDALSLEIPRG
                     LPPARTQMQDVLSLALETLSVPTRSLILGGFSQGAMLATDLALHTDLPLAGLALLSGT
                     LIAKQEWLTRLPTRQGLRVFQSHGTDDPILSFSMAEQLREHIQTAGLPVSWVEFRGGH
                     EIPIQVLKGLEAFLQASLYPPPH"
BASE COUNT         3063 a         3226 c         2811 g         2871 t
ORIGIN      
        1 tccttggagt cacccagatt caatgctccc aggctccatc ctccacccct gctgagcctc
       61 ccccatccat aaccgcagcc ccaaatggaa ccgatgtgcc tgttgcggtc gtccatgcag
      121 ctgattcgtc tcccgtggtt aaggcatcca cccctcttct agcctccaac gaaacctttg
      181 ttaaaatcgc caaagaggct atggcctcgg tcgtgaatat ctccgcgacc aaacgcaccg
      241 tccaatctca aggcacgaat cccctgcttg aagatccatt tttccggagg ttttttggag
      301 aggaatttga gcggcggttt aaacaaccac gcgaacggca ggagcaaggc ttaggctccg
      361 gagtgattgt cagcgatgac ggatacatcg tcaccaataa tcacgtcgtg gaacaggccg
      421 atgacttaat ggtcctctta ggagataaac ggaaacttcc ggcgacactg attggcacgg
      481 atcccaaaac cgatttagcg gtgatcaaga ttgaagccac cggattatct accttacctt
      541 ggggaaactc tgtggcactg gaagttggag aattggtcct ggcggtcggc aacccctttg
      601 ggttgaatca aaccgttacg atgggaatta ttagcgccgt gggccgggcc aatgtcggca
      661 ttgtcgacta tgaaaatttc attcaaaccg atgccgccat taatccaggc aattccggag
      721 gtgctctcgt caatctgcag ggccaactca ttggtataaa taccgccatt ttttccagaa
      781 caggcggcta catgggcatt ggctttgcca tccccagcca aatggtaaaa agtgtgatgc
      841 aaagcctgat cgggcatggg aaagtcattc gtgggtggct gggcgtgtcg attcaggaat
      901 tatcccaaga cctcgcaacc caatttgatg tgcctgatac ccagggcgca ttggtgggtg
      961 atgtgtttag cgaaagccct gcaggacaag ccggattgaa acgtggcgat atcatccgca
     1021 cctacaatgg tgtcaccgtg aaagatccga accatttgcg cacacttgta gccgagacga
     1081 caccggagag ctccattccc cttgaagtgt ggcgaaacgg aaaaattgtc aatctctcgg
     1141 tttccatcac tgaaatgcct aaagatactg caggattgac aggagagtct cctagttcgg
     1201 tctcaggtaa ccacgccctt tcagggcttt ctgtagaacc cgtccagccc ggacaaacac
     1261 gagatggtca gggtgtagtc gtgacccaaa ttgccccgaa ctctccggct gaacgggccg
     1321 gcgttcaacc gggtgatgtg atattggaac tgaatcgctc tcccattggg tccgtgaggg
     1381 atttcgaaca gcttgtgaag cgattggaaa ctggaacctc cgtcttagtg ctcttgcagc
     1441 gcggaaaagg ggccattttc ctgaccatca aaccatagcg aaagggccgg aggctctcct
     1501 tcgccagacc tgaccacttg agggccgaac aaaccatgac attactttgt tcattcagaa
     1561 cattccttca aatgagtcaa ctgctctgct tcctggtcct tctgctattc cccgtttcca
     1621 cgcaaccaat tttgtgggcc tcttctccac agattgatat tcaatcagca cccccatttt
     1681 tttctcccaa ccacctgatg atccgcttgg ataccgccct cacgtggaaa aataacacag
     1741 gtgaagccca ttccattgtc gcagacgact gtcaatcccg ttcacgatgc tcatttgact
     1801 caggagtcat tcgtcccgaa aatcagtatg tcttaacccg gttagcccct ggtcgctatc
     1861 cttaccattg tggactccat cccttcatgc gaggcctatt aaccgtccaa acaacggcct
     1921 ctccatcctc tgatatctga tttaagatac atttttttgt tcttgcgacc gtgttctccc
     1981 tagtcaacac tggtgattta cattcttggc ccatgcatcc gccgtttttt cattgcatgc
     2041 aataagtccc taatggtaca tttcgggcat ccctggtaag ggcagaaaaa acaaatatca
     2101 agtaacgaac agactcagac tggtcccatc gtctaggctg gtctaggacg gcaccctctc
     2161 aaggtgcaga cacgggttca aatcccgttg ggatcaccaa tttccatagg aacggacgtt
     2221 cgcccccttc ctggttgttc gcattcgcac ccgttagaat cagactcctc gtactcccgt
     2281 ctggttattt ctttaatcta tgccgtaagg atctgtgatg acgacgagaa cttttcacga
     2341 tggcgaaagc gacgggctgg aagccctgga ggctctggat ccggaagcga ttgaatcttt
     2401 ttccgatctt ctggcggcca tgaaaaaaac ggcctttggc gggcgccgtc ttggcgatgc
     2461 ctttgagatt ttaaaaaaaa tggtcgagga tcctgattgt actgtgatct tgaccttgtc
     2521 cggggccatg accattgcaa aaatgggaaa gattatctgc agcatgatcg accgaaacat
     2581 ggtgcaggcc gttgtatcca caggggcatt gattgctcat ggcctgagcg aagccgtagg
     2641 taaaacgcac tataagtatc acccatccat gacggatacc gagctctatg aaaaagggta
     2701 taaccgggtc tacgacacgc tggagatgga aacaaatctc aaccacgtcg aacgggtcgt
     2761 cagtcaaacc ttaaagcgga tgactcctga aatccccatc tcctcggaaa tcctgactcg
     2821 agaaatcggg aaaaccctcc acgacgatta cgaaggccca ggtattttaa aaagtgccta
     2881 tgagcagggt gtcccggtct atatccctgc atttaccgat tctgaaatgg ggctggatgt
     2941 ctccatttgg gcgatgaggg aaaaacgaca atctcaagcc caacctccac ccgatctctc
     3001 cgatcaggaa acctggcagc ttttacagca atctcgtccg gcgtttaatc cattcttgga
     3061 cctcaatagt tatacggaaa agctgctgtc tgccaaacgg ctagggattt tcaccattgg
     3121 tggcggcgtt ccccggaatt gggcgcaaca aacgcccccc tacatcgaga ttctcaatct
     3181 ccggatgggt acacaattaa cccctccgcg ataccagtat ggtgttcgca tttgcccgga
     3241 gcccgactac tggggtggcc tcagcggatg cacgtatgag gagggtgtat catggggaaa
     3301 atttgtgcca agaaaagagg gcgggatgtt tgctgaggtt ttgagtgatg ccacagtggt
     3361 ctggccactt ctgatgatgg gacttctgga aagtgcccgt cgcaggtaat tcaacacctg
     3421 gcagacgacc aacttcttat ctcgacatgg tccgtcagga atgcaacaat attggtaagg
     3481 gagtgatgat gaatattggc cagtccctca aatccaccat agccttcgcg atccttctcg
     3541 gcaccctgct catcagtccc atgactcatg ggaaaactcc ggattttttg atccaggatg
     3601 atgatgtggt gcgaggggat cctaaggctc cgatcaccct gctggaatac tcggatttta
     3661 cttgtgggtt ttgcgaaaag ttttttcatg agacctttcc caaattgctt tcagagtata
     3721 ttgagaccgg aaaagtgcgg tttgtctatc gggacttccc tcgaggcatg ggaagcccac
     3781 tccgggcagc cgatgcggcc cgatgcgcag gagagcagaa tgcctattgg cccatgcatg
     3841 atcgtctttt taacagtgat ggccagttgg ctccggataa tctcaagcaa tatgccaagg
     3901 acctcaacct ccacgtcgag caattttctg aatgcctggc ttcccatcgc tatttgaaag
     3961 atattgagaa ggatttacag gatgccggat ccttaggaat tcgtggcacg ccggcattcg
     4021 tcctctttcc cacaaccctt ccggaagatc ctcatctcat tttaattcca ggggcctttc
     4081 cctatgagac attcaaggaa gaaattgata agttgctcac gcttcatgga gcctgaacct
     4141 cctcgatttt tttaccggta ccgtcctcta ctcacatcga agagacataa atccttcatt
     4201 cattcgctct ccacgcaatt cctcattgat ctgacagcgt gattaggtgt acatttgcac
     4261 aggtaatttt gacattcatg ttttaactca gaggacgggt ttgatgtcat gactgaatcc
     4321 caatctttct ggcctgttga atgcgcacaa ggtgaaccgg acttatttgt gtgcttaacc
     4381 tgttttgatg aagtattcaa agccaagatg ccggtcgacg ggtgtccgag ttgcggagcc
     4441 attgccgcat tcgaaccgtt tagtttagac gcgatcaagg aatggggaac ggaaaatctc
     4501 attcagaaag ccgaacattt gccttcctcg tctgatcccg gttctgacca gccaccttca
     4561 tcaatctaat cacctctatt tcacgaggtt ctcacctact catgactgtg gccacggcaa
     4621 agccaatcct tataggcggt caatgggtca agaccccatc tgcggagccg gtacgtaatc
     4681 cctactccaa tgaggtgtta gccatggtct gccaggctga gcctgagcat atacaccagg
     4741 tcaccaactc cgccaaacag gtctcatccg aatgtgccag aataccggca catgccagag
     4801 caaaggcgct cctgcacatc gcgaccggcc tatccactag acgggaagaa tttgcgtcca
     4861 caatttgtca ggaggctggc aagccaattt ccgacgcacg gcgtgaagtc gaccgagcca
     4921 ttcagacttt tcgcctggcc gcagaggaaa gtactcgcat cccgggagaa atcctgccga
     4981 tggatctcac cccaggaggg gaggcatact caggatctgt cacacgcttc ccgatcggaa
     5041 cggttcttgg cattacccca tttaatttcc ctctcaatct ggtagcccac aaagtggccc
     5101 cttgcctagc ggcggggaac gcgatggtat tgaaaccagc cccgcaaacc ccattaactt
     5161 cgctcatgtt gggagaagtt tttttgacga cggaactgcc tccggaaatg ctgacgatca
     5221 tcccctgttc caataccctg gcagaacaga tggtgatcca tcccaacttt caagccttga
     5281 gttttaccgg ctctatgacg attggatgga tgctgaaagg aaaagccgga tacaaacggg
     5341 ttctactgga actcggaggc aatgccgggg tgatcataga accggacgcc aatctcgatg
     5401 tagccataga ccgttgcgtg gcgggagggt acggatatgc cggacaaacc tgcatttctg
     5461 tgcaacgaat tttcgttcat gaatcgcggt acgacgactt tcttaaggcc tttgtcgacc
     5521 gcgttcgatt attgccgatg ggcgatccct cgctcgagaa cattgtcgtc ggtcccctca
     5581 ttagcgaaca agccgcacgc cgagtggagg catggatcca agaagccgtt tcgcaagggg
     5641 caagagtcat gaccggcgga acgcgaaagg attcattcat tgaacctgca gttctgactc
     5701 acgtcagcaa caacatgaag gtctgttgcg aagaaatttt cggtccagtg gtcactgtca
     5761 cgccctatgc aaacctcgaa gaagcatttg cccttcacaa tgattctgat ttcggcctcc
     5821 aaacaggaat attcaccagc aacatcgatg cgatgtatca ggcatactca cgattagaag
     5881 tcggtgcgct tctggtgaat gaaattccga cttttcgagc cgatcacatg ccctatggag
     5941 gaatcaaaca gtcaggatta gggcgagaag gtgttcgata tgctattcag gaattgaccg
     6001 agcctaaatt gttgctgatc aaaagatctt cttgaggcct tcttgttttt tcttgaacgt
     6061 tgagtcctca cgatcttgca ccttgccaat cactcttggt aaaaatatcc ggctaggtga
     6121 caaggttgtt ctttcccgtg gccgtccgaa tcgtccagga tttcgcattt tcatacacaa
     6181 attggaaagc gaggtcggga atcagattcg acgatcaaac aggcatgtac tgcctaaccc
     6241 attccaccac taactgatta atgaaggagt agagatggtc ccaacgcaaa tgtttctcac
     6301 cagaggtgtg ggtgtccaca aggaaaaact gacctctttc gaagaagccc tccggagcgc
     6361 aggaattgcg tattgcaacc tggtgaccgt ctcatccatt tttcctccag attgtaaaat
     6421 catcccgaga aaacgcgggg aacagttact caatccaggc gaaattacct tttgcgtcat
     6481 ggccagatcg gacacggatg agcggaatcg cttgatttcg gcctcaatcg gcctagccat
     6541 tccctctggc aggaaagtca attacggcta cctgtcggaa catcatggct atggggaaac
     6601 ggacgaagag gctggagaat ataccgagga cctcgcagcc caaatgctcg caaccactct
     6661 tggcatcaaa ttcgatccaa atgtggcctg gaaggaacgt gagcaggtgt ttaaaatggg
     6721 aaaggatatt gttcgaactc agaacattac ccaatccgcc atcggaaaac cgaacctctg
     6781 gactacggtg gttgcctgtg cggtcttcat tcctcttgaa aacattactg aagatccaaa
     6841 gcctcgacac aaaaaatcga aataggttcg ttgatagggt aaccattcgg gtgaatgctt
     6901 acttctgccc ttaggtcttt agccacaaaa caagatccaa ttcgaacatc atttccacgc
     6961 aataggctta ggccttattt accaagggtc ggttctgaac aagaaccgac ctttttctct
     7021 tacagtgaaa attccgccca taaaaccgta tggtcagatg gtgactcctc ccgtcgaggc
     7081 tgaacatcca ccttcaccgt atgggaatgg gaaaggagtg gaggagtgac cagaatatgg
     7141 tcgattcgcc atccacggtt cgcctccaga gcttctggtc gtcgataatc ccaaaacgta
     7201 aattgctgtt gatgcggaaa atgatgtcga aacacatcaa cgaatcccca ggacaccgtc
     7261 tcctgatatg cccgacgcac atcttcatgg aaacacacat gcttaagatg tttttcgggg
     7321 ctatgcacgt caatcggctc aggagcgaca ttcatatctc cacaccaaat cacaggttga
     7381 cctgttgagc ccacacgcga aaaatacctt ctcaatctct gaaaccactc taatttatag
     7441 gcatatttcg gggaatcaat caaaaagccc tgcggaatgt aagtattaat aatggtgata
     7501 ccattgatca ccacccgagc caggcgtgcg ggatcttctt gtggctcccc gtcatctaaa
     7561 ccgaatgaca ccagttccgg tggtgtgcgg gtaaaaatgg cgaccccatt gtaggatttc
     7621 tcaccacaaa aattgatgtg atagggtaaa tcggcaaacg cttgcagggg aaattcatga
     7681 tcttgcactt tggtctcttg aagacacatg acatccggat tgtgttgcca taaccacttg
     7741 tgaagaatac cgatccgttt tcgtatagaa ttgacattgt aggtcgcaat tttcattcgt
     7801 cccgattctc ccccattcac cattcacgcc gcagacattc ctcaaaaaag acaaagcccg
     7861 attctcttcc acaacccatc cttaagtatg tgaggtatat gaatttatga ccactagtca
     7921 ttccttcact cctgatgaat taaccctctt ggccgatcgc gattttttcc gtaagaaagc
     7981 caaggtttcc cttaaaatca aacagatcct ggaggagctc caggaacgcc ttgaggctga
     8041 aatcgcgttc cagccccttc tcgctccaga aggattcgat tctcaagcca ggcaatttgt
     8101 caaaggcgag catcttgaag atttccccta tcaatatgtg gatttcccca gattgtatac
     8161 acgtgagacc aagtttgctt tccgatcctt gatctggtgg gggcaccaca tcgtcttcgc
     8221 cctcatgatt gaaggcccgc tgatcaaaca atatcgacgc aacctgttta atcgatactt
     8281 agaggtagca gaccgggatc tctgcctctg tctcagcccg tctctctggg agtggaaata
     8341 cggaccaggc ctcactcttc ccatcacacg cagtcgacga tctgaaatcg ccgcaacact
     8401 cgaccatcgc acgttcttta aaattgcgcg gtttcttcca atcgatgatt cggcagtgga
     8461 aaccggcacc atcgtcgatg aagggttgaa agcctttcag gccatcctac cggtcataag
     8521 aacataatct cagacgccat ttccctcatt ggtcattccc acaacatgac ttcctgtcct
     8581 atctttattc ttcttcagca aggccaagca gttcggtcag ttgatccacg gaataggact
     8641 gtgtgacctg aagagtaatc gattgaattc ccgctgcctt caacagggtc tccacaaatc
     8701 gatcccggtc ctcgcgtccg accgcagaag gaacctcctt ctccaagtta accaccgtca
     8761 tgactttaag agaaccagga tgggcaagaa cgacatcgca gcgaacagac tgaatcgttc
     8821 gcataaccgc ttttcgagct tcttcatctt tatcgacaac agacaggaca ctgaggaggg
     8881 ggattttagc caaaatcaac atatggtcac gtgccgctag ggtaatcaga ttgtacaagg
     8941 tcgcttctcc cggcgacatg aggggtcgag cagtcaccac gctctcagca gattcaggag
     9001 tcaacggtgg aagagccccc gctggaaggg gacggcgcca gatccatcgc aacaaaaaca
     9061 cggctccgag tatgaagccg gccaggagaa tcagtgaaaa gctgttttga gacatcaatt
     9121 cgtcagtcat ttcgccagcg ggaacaatcc cttaacaatg ggaagcactc taaggaagtc
     9181 cgtgaatacc gggagtgacg gtccccaaaa taaccggatg gcgagcccct gtgaccttag
     9241 gaagattggc acagacacca tgaatggttt gatacgccag caccgcaaag gcctgcgctt
     9301 caaaggcttg gctggagacc ccacattcgt ccatacacct cacgggaatt ggatcgaaca
     9361 caccggacag ttctgaccag agtcgagtat tccgcactcc accaccgcca aacaccaact
     9421 catcagggat ctccttcgtc catttttgcg aatcgtgaat tacctgagca ataaaccgac
     9481 agctcgtcgc taaaaggtcg gaaaaggcca aatggcgacg ctgcgcctga gcccacactc
     9541 gctggatata gccttcccca aacatctcac gcccggtgga ttttggagga cgtcgagaca
     9601 tataaggatg cgcaagcagc catcgaagca gacccttatc cacctgaccc ttcagggcaa
     9661 gacgaccacc ccggtcaaat ctggcttgcc cctgtgtctc catagccacc aatccatcca
     9721 acaacatatt gcagggtcct gtatcaaacg cccgaaccgc cgccaagttt cccccgccgg
     9781 gtagtacggt cacattggcg atccctccga gattgaccac cattcgtgtc gcacgtttct
     9841 gagcaaaaag aagagcatgg acataaggcg ccaatggagc cccctccccc cccacagcca
     9901 gatctctagc tcgaaaatcc gatacagtcg tgactccggt acgttccgca atcacattgg
     9961 gatctccaat ttgcagggtc gaacggatct gcccgatacc aggctcataa atagcggcag
    10021 gccgatgatg aatcgtttgg ccatgcgaac caatcaacgc cactcgttga ggagaaatgc
    10081 cggaatgttt gatggctaac attgcggttt tggcaaacac ttctcccaaa accgcattga
    10141 ggtgacagat ttgccctacg gtcccatgct cacacgcttg taaaatgagt tgacgaagcc
    10201 tgggagaata agaacggcca acatgtttga gtagtgaact cttgagacga tgtccgcttc
    10261 cccgaatatc cacgaccgcc gcgtcgaccc catccgccga cgtccctgac attaatccca
    10321 caactatcac tggtcacctc gttttcctgg tctggatgcc gcaatagcat aaccatggac
    10381 acgttccaat ttctccagac ttcatctcta cctattgaat ggttccgtgg gaggaacgat
    10441 atgcttgccc cggtcccaga ccataccagt ctgctcttta tcagaaaatc ccaaagaggc
    10501 gtaaaacccc tgatagcgcc tggtgcaaag ccaaaacaac tccacccgtt ccagcttggg
    10561 atgccttaat attcgttgga taatgccttt accgatatcc cgatcctgat aggcacgatc
    10621 cacgatcaca tcccaaatcg aggcgcgata gatgaaatcc gtcaacaccc tcccaaatcc
    10681 gacgagcctg ggaccatccc aggcggaaat ggccacatcc gtatgtgcca gcatccgttg
    10741 agtatcatcc aatgaacgag cacaagccca atccgtttgg cagaacaatg ccaaaagctg
    10801 ttgcgcctga atgtcgtggg aatccaaaaa attgatcatg cgttggcaga ttgtggcaag
    10861 atgtattaca ctaagtctac tagaattaac tgacaaaaac cggagtcgtg ccgatgtcga
    10921 tgttagtcag aaaatgccca aaatgcagta agctattttg gaaaaaagaa gaagagatcc
    10981 aatatgccga cgagaatacc ttgaaagtcg tctgcaatca ctgccaccag gtcttgcgga
    11041 ttccgctcat cacagaaggc gccaatgccg gcgccccgaa aatgggccat taggatttta
    11101 aataattcgc cttccagtat cgcattcacg gatttaccaa tactacacaa ccaatcaaaa
    11161 atttcctcct tctctcgtct attccactag acttctgact gtatgcgaca cgaacattta
    11221 gccggtttag atgtatgtat tacaggtgga gtggatcgcc aaggaagcgg cgacggacca
    11281 cttgtagttc tcttgcatgg attcggtgcg ccaggggatg atttagtcgc attatggcgc
    11341 tatctcaatg tcccggatga agtccgcttc ctttttcctg ctgctccact cgaactgggc
    11401 atgggctttg ggaacgctcg agcctggtgg atgctggaca tggaacgcct cacacaggcc
    11461 agagcccaag ggcagtggga tgcgttgtca ctagaaattc ctcgtggcct gcccccggcc
    11521 cgcacacaaa tgcaggacgt cctctctctc gccctagaaa ccttgtcggt accgactcga
    11581 tcactgatcc ttggagggtt ttcccaaggg gccatgctgg cgactgacct ggcccttcat
    11641 accgatttac ctttggcagg cttggcactg ctatccggta cactcattgc caagcaagaa
    11701 tggcttaccc gcctgcccac tcgccaaggt cttcgggtat ttcaaagcca tggcactgac
    11761 gaccccattt tatcgttttc catggcagag caacttcgtg aacatatcca aacagccgga
    11821 ttacctgtca gttgggtaga gttccgtggg ggtcatgaaa ttcccattca agtgttgaag
    11881 ggcctcgaag cctttctgca agcaagtctc taccctcctc cgcattaata aaaaaaataa
    11941 attcggtaaa cagttaggac agaaggcaaa a
//
LOCUS       DAORAQ010000073        11767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_898_length_11767_cov_57.215420, whole genome shotgun sequence.
ACCESSION   DAORAQ010000073 DAORAQ010000000
VERSION     DAORAQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_11767_cov_57.215420"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1445
                     /locus_tag="PKM72_08200"
     CDS             63..1445
                     /locus_tag="PKM72_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60804.1"
                     /translation="MRNPSFILMGWMVAAMVGVGVAVARAWPFWVVLLLGAGLAGVAA
                     VMTRNTWKQTRKNHEDLMTGLRQLEQLSETAEARASRVLEPDALGGHIQRLVEDVKQR
                     MTRLEEERTKIIAIMENLVEGVVAFDSKGKVLFTNSSAHRILGLDAKAIQGRSVWEII
                     RNQELAGLVEGCQELAWHERREAEVELYPPVSMVLEVYALPFPISNQKKGSVLVLHDV
                     TELRRLEQVRTEFIENVSHELRTPLTAIVGYLETLVDEPSLETPNNRKFVHVAYQHAE
                     RLSRLVEDLRSLSEIESGKILLRCELVPLREVAQDVCEMFQNQLSKKNLQISNEIGIE
                     NSAWADRDRLIQILVNLVDNAIKYTPAGGRISFQAMPFRDQEVCLQVSDTGQGIPSID
                     LPRITERFYRVDRARSREEGGTGLGLSIVKHLLQLLGGKLRVQSELGKGTTMEVILPK
                     SRPTPTTKPL"
     gene            1643..1954
                     /locus_tag="PKM72_08205"
     CDS             1643..1954
                     /locus_tag="PKM72_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60805.1"
                     /translation="MATSHKEWTIKNLVERGDRDMVLGVISAKQSVTIEELSNDLPWM
                     RWGYLFSILGECLHEGLVILCQKEFQFEVRAIHALSSGNGEDVLTGSFSSKPGDRQMA
                     I"
     gene            2032..3045
                     /locus_tag="PKM72_08210"
     CDS             2032..3045
                     /locus_tag="PKM72_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNP60806.1"
                     /translation="MEHSQNRKQMWVLGMSMGLILGWSATTILGASHVLANDDMEDPA
                     WVNYAKVSGVSGNINSIGSDTLNNLMTLWAEGFRKQYPNVKIQIEGKGSSTAPPALIE
                     GTAQLGPMSRKMKGKEIDAFEAKFGYKPTAIPVAVDALAVYVNKDNSLQGLTIGELDA
                     VFSKTRQHGYKENLTSWGQLGLTGRFADMPISIYGRNSASGTYGFFKDVVLENGDYKD
                     EVKEQPGSASVVQGVTEDQAGIGYSGIGYRTSGVHALPLAMKAGQPFVEATYENSTSG
                     KYPLSRYLFVYVNKAPNQPLSPIVREFLKYVYSREGQKVVIKDGYFPISEKIIGKQSE
                     HLK"
     gene            3158..5467
                     /locus_tag="PKM72_08215"
     CDS             3158..5467
                     /locus_tag="PKM72_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNP60807.1"
                     /translation="MPPLSSSSSSGALVPQKNSLAGSSRVVAQRRIRHLLDYVAKYVV
                     GAGGLATIVSILGIFVYLVWEVIPLFQPATVTQELTVPIPVKAIQSSNVAKAMVGIDE
                     YREVPFVLKGKYLQFLSLSDGAAIPDVGGELSIDGDPTSVVRMGLKGHSLSIGTDQGL
                     VYPVNILYRSDFQGDRRTIIPSVKVKEPVRVVPDGMGIVLHAHVMRSDEFQTVVAFTG
                     GGELWVTRIEEPGDFSFSDEVVVETNRLVLPPKFHLTTLALDSVGKRLVAGTRDGYLL
                     TWEWRGQGFDAMPQSVPVGAAGDAVTVLSYLLGERTLVVGTTSGSVSTWAFSADQSGP
                     SGRFLRKVHTFTPHARAVTSLSVSQRDKGFLTADIQGTMFLHYSTTGETILDIPGNGN
                     ALESLRYAPKADGLVWLGEDGTLQTYAIDNPHPEITFKSLFFPVTYEGYDHPEMIWQS
                     SSGSDEFEPKLGLIPLMFGTLKGTLYAMVLAIPLAVMGAIYTAMFMHPHLRSVIKPSL
                     ELMAALPSVVLGFLAGLWFAPLLEKIFPAVVAIVGFLPVVIAACCLAWQFLPIRVRRL
                     EVYGLDLMVMMIAIVLTVGACLIANQAIEGWIFGGNFKQWLAQNLGLTYDQRNAIVIS
                     FAMGFAVIPIIFSIAEDAIANVPRQLVAGSLALGATRWQTLTRLVLISASPGIFSALM
                     IGFGRAIGETMIVLMATGNTPIMDWSMFNGFRTLSANIAVEMPEAPHGGTLYRVLFLS
                     GLILFGFTFTINTIAEIVRQRLRQKYSQF"
     gene            5478..7109
                     /gene="pstA"
                     /locus_tag="PKM72_08220"
     CDS             5478..7109
                     /gene="pstA"
                     /locus_tag="PKM72_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNP60808.1"
                     /translation="MKFLQRFFASGTLFIWLSGGALACSLLLIGGLLMLIFINGMGYF
                     WQKDLVQLTLNDNTRVLGEIVDTQRIPDTVTADFPDGQSRIQLKVGNRDVYGLDFRWV
                     DEADIAQRDWPQDVIAFERREWGNFYGWVTAVWDGDSRLAGGRDIAWEVWYPFLEQAE
                     SLYQEIHHIERDVIGAINADMERARLSLKTLELKGDISQDSADSLQRDIQEREAAYQE
                     QIQRLVGLYERLNRYRVTLMDVTGKEKTFPVGGLVRAWRPNSMSVWDKTTMYVENFWN
                     VLSDEPREANTEGGIFPAIFGTVMMVLVMSVVVVPFGVLAAVYLKEYAKQGLLTRMIR
                     IAVNNLAGVPSIVFGVFGLGFFVYAVGGSIDQLFFSASLPNPTFGTGGMLWASLTLAL
                     MTVPVVIVATEEGLSAVPREYREGSLGLGATKFESLMRVVLPCAMPGILTGLILAVSR
                     ATGEVAPLMLTGVVKLAPALPLDGQFPFIHLDRKFMHLGFHIYDVGFQSPNVEAAKPM
                     VYVTTLVLVLVVLLLNMTAIALRTRMRKRYAGASV"
     gene            7225..8031
                     /gene="pstB"
                     /locus_tag="PKM72_08225"
     CDS             7225..8031
                     /gene="pstB"
                     /locus_tag="PKM72_08225"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNP60809.1"
                     /translation="MKKTFVESETVQHPLTDSPAKIVIQDMNFYYGTVQALKSVNMTI
                     PANKVTAFIGPSGCGKSTLLRCLNRLNDLVDGTRVEGTILLDDENIYRPEVDATDLRT
                     RVGMVFQKINPFPKTVWQNVAYGPQLQKIRKRATLDAIVETSLQGAGLWDEVKDRLHV
                     SALGLSGGQQQRLCIARALAVNPEVLLMDEPCSALDPISTGRIEELLHTLKERLTVVI
                     VTHNMQQAARVSDLTGFFLLGELIEFEDTKKIFTNPGDSRTEDYVTGRFG"
     gene            8061..8720
                     /gene="phoU"
                     /locus_tag="PKM72_08230"
     CDS             8061..8720
                     /gene="phoU"
                     /locus_tag="PKM72_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNP60810.1"
                     /translation="MKRHFDDSLADLRQRILRMGALVEGQIRQALIALVDRDDLVANQ
                     VIQNDRQVNTMDVAIDELCLELLALHQPAAKDLRFVTTAMKISTELERMSDLAENICE
                     RALELHTEPQLKPYIDIPLMAERAIKMVGEALDAFVRGDSVLARQVLKEDDYVDELNE
                     QIFRELLSFMMENPQTISRGIRLSFISKYIERIADHATNVAELVVYMVEGKIIRHMIP
                     T"
     gene            8898..9965
                     /locus_tag="PKM72_08235"
     CDS             8898..9965
                     /locus_tag="PKM72_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNP60811.1"
                     /translation="MQKWVDTRGGSSQYSKGQATHLPSQGSGSLWSLVLAGGHGERLR
                     QYTEQCFGAYRPKQYCAFLGKRSMIQHTWTRAKALCLPHQVVTVMDQSHWGYVRTQLI
                     QESLGRLVWQPDNRGTGPGVFLPLSYIRAHDPSATVVLWPSDHFIFPVEPMLQVIRDA
                     AALVQEHPYRMVLLTVPPQSREQDYGWVIPGDRIPHSGGSRVREVRRFVEKPMGLQLE
                     DIALSGGQWNTLVLIANVQTLWEAGRQCIPEVVMPLEEHACGIGTSNEQQRLLHLYRT
                     MPTRNFSSDLLALIPQQLHMMDLTGVHWSDWGRPERIQETVAYMRRHGSIGTRGGSPS
                     LVWMEPRQDKAVPEELSVLTR"
     gene            complement(10365..>11767)
                     /gene="ppk1"
                     /locus_tag="PKM72_08240"
     CDS             complement(10365..>11767)
                     /gene="ppk1"
                     /locus_tag="PKM72_08240"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNP60812.1"
                     /translation="FRITRDADFEIEEDEAGDLLAAIEEQIDLRQFGSVVRLELDRDM
                     PDSIKDILIRNLNLAPYQVYTYNFRLGLSNLLELTSIDRPNLKFPPHFPNILHDPASK
                     ESLFSLIKREDIFLHHPYDSFTPVVDFIRQAALDPKVLAIKQTLYRVGINSPVVDALM
                     EARQNDKQVAVLLELKARFDEENNIEWARKLEDEGVHVVYGVLGLKTHAKVCMVVRRE
                     SDGIRRYLHLGTGNYNPITSKFYTDFSLFTCQPVFGEDITDLFNALTGYSKKDSYAKL
                     LIAPVGIRTNIIDRINREISRQQQHGDGYLAFKVNSLVDKASIQALYRASQAGVRIEL
                     MVRGMCGLRPGIPGLSQTITVTSLVGRFLEHSRCYYFRNGGQDELFLGSADLMPRNMD
                     RRVEVLFPIENSTLRTFIVETILRPYLKDNMHTRQLQSDGSYTRVKPLENEAPFHAQD
                     WFIANWKGKHTKPPSL"
BASE COUNT         2714 a         2626 c         3304 g         3123 t
ORIGIN      
        1 gtggggtatc gttttgagtc gggggctgtc taaggccagt atgatgaaag tgacctcgat
       61 ccatgcgtaa cccttctttc atcctcatgg gctggatggt ggcggcgatg gtcggggttg
      121 gcgtggcggt cgcccgggca tggccattct gggtggtgct tttattgggg gctggtctgg
      181 caggtgtggc tgcggtgatg actcggaata cctggaagca gacgaggaaa aaccatgaag
      241 acctcatgac tggccttcgt caacttgaac agcttagtga gactgctgag gcgagggcct
      301 ctcgtgtgtt ggaacccgat gcattgggag gccatattca acgcctggtg gaagacgtga
      361 agcaacgaat gacccgtctc gaggaagagc gtacgaaaat tatcgccatc atggaaaatt
      421 tggttgaagg cgtggtggcc tttgattcca agggaaaagt gttattcacc aattccagcg
      481 cccatcggat attgggattg gatgccaagg cgattcaagg acggtcggtt tgggaaatta
      541 tcaggaatca ggaactggca ggtctggttg aaggttgtca ggaattggcg tggcatgaac
      601 gacgagaggc tgaggtcgaa ttgtatcctc ccgtctccat ggtgctagag gtgtatgcgc
      661 tcccttttcc tatttccaat caaaagaagg ggagtgtgtt ggtgttgcac gatgtcacgg
      721 aattacgtcg attggaacag gtccggaccg agtttattga gaacgtgtcc catgaattgc
      781 gtactccgct cacggccatt gtcggatatt tggaaacgct tgtggatgag ccgtctttgg
      841 aaactccgaa caatcgaaaa tttgtgcacg tcgcctacca gcatgcggag cggctcagcc
      901 ggttggtgga agacttacga agcctctcgg aaattgaatc gggaaaaatc ctcttgcggt
      961 gtgaattggt gccgttgcgc gaagttgccc aggatgtctg tgagatgttt caaaatcaac
     1021 tgtcgaaaaa aaatctgcag atttccaatg aaattggcat tgaaaacagt gcctgggcag
     1081 atcgggatcg gttgattcag attctggtga atctcgtgga taatgccatt aaatatacgc
     1141 ctgccggagg acggatctca tttcaagcca tgccttttcg tgaccaggaa gtctgcttgc
     1201 aggtcagcga cacgggccag ggtattccgt caattgacct tccacgcatt accgaacgat
     1261 tttaccgggt ggatcgagcg cgttcacgtg aagaaggcgg gacgggactt ggactttcta
     1321 tcgtgaaaca tttactccaa ctgctcggtg gtaagcttcg cgtacaaagt gagctgggca
     1381 aaggcaccac gatggaagtg atcctgccga aatccaggcc aactcccaca actaaaccgt
     1441 tatgagatct cacattctct cccccgcagc ccgcttattt taattttttc ccttttcgtt
     1501 cccctgaaaa ctttacacag ttgtaacccg aacctcatcg ggctgcaaca gttgatcgct
     1561 aggatgccat tgaatggttg attagcctga atacctcgca tggacctgcc tcaatattgg
     1621 ataacgacta caggagggga gcatggcaac gagtcacaag gaatggacga tcaagaatct
     1681 tgttgaaaga ggagatcgtg acatggtctt aggcgtcata tctgccaaac agtccgtgac
     1741 gattgaggaa ctctcgaatg atttgccctg gatgcggtgg ggatatctgt tttccatact
     1801 tggggaatgc ctgcatgaag ggttggtgat tttgtgccaa aaagagtttc aatttgaagt
     1861 cagagccatc catgccttgt caagcggaaa cggggaggat gtcctgacgg gatcattttc
     1921 gtcaaaaccg ggagatcgac agatggccat ttaacacctt tgtaacattg ccgttacata
     1981 gaagaaacct gttggaccta cactattcat tcacatgagg aggtcagttt gatggaacac
     2041 agtcagaatc gaaagcaaat gtgggtgttg ggcatgagca tggggctgat cctgggttgg
     2101 agcgcgacta cgatacttgg ggcctctcat gttttggcta atgatgatat ggaagatccc
     2161 gcctgggtga attatgccaa ggtgagcgga gtgtccggga atatcaatag tattggctcg
     2221 gataccttga ataatctcat gacgctgtgg gctgaaggat tccggaaaca atatccgaat
     2281 gtcaaaattc agattgaagg gaaaggatcc agcaccgctc ccccggctct tatcgaaggg
     2341 acggcgcagt tgggcccgat gtcccgcaag atgaagggaa aggaaattga tgcgtttgaa
     2401 gccaagttcg gctacaagcc caccgccatt cccgttgccg tggatgcgtt ggcggtctat
     2461 gtcaataaag acaattccct tcagggtttg acgattgggg agcttgacgc ggtgttttcc
     2521 aaaacccggc aacatggata taaggaaaat cttaccagtt ggggacaatt gggtttgacc
     2581 ggtaggtttg ccgatatgcc gatcagtatc tatggccgga attccgcctc ggggacttat
     2641 ggatttttca aagatgtggt gctggagaat ggtgattata aagatgaagt gaaggaacag
     2701 cccggttcgg cttctgtcgt gcaaggcgtg accgaagatc aagccggcat cgggtatagc
     2761 ggtatcgggt atcgcacatc gggggttcat gcccttcctc tggcgatgaa ggctggtcaa
     2821 ccgtttgtag aggccaccta tgaaaattcc accagcggga agtacccctt gtcacgatat
     2881 ttgtttgtgt atgtgaacaa agcacctaat caaccgttgt ctcccattgt tcgggaattc
     2941 ttgaaatatg tctatagccg ggaagggcag aaggtcgtca ttaaagatgg ctatttcccc
     3001 atctcagaga aaatcattgg gaaacagtca gagcacctta agtaggaatt ccttgatgac
     3061 cctccctacc gttttccata gaaacccttt ggagagcggt tgggtgttct gatctcatct
     3121 tctgcttctt tttcactttt gccgaactca aaccctcatg ccacctttat cttcctcttc
     3181 ttcatcgggt gcgttagttc ctcaaaaaaa ttcgttagcc ggcagttctc gcgttgttgc
     3241 acaaaggcgg attcgtcatt tactggatta tgtggcgaag tatgtcgtcg gggcgggagg
     3301 cttggcgacc attgtcagta ttctaggaat ttttgtgtac ctggtttggg aagtgattcc
     3361 tctgtttcaa cccgcgaccg ttactcagga gctgacggtg cctataccgg tcaaagcgat
     3421 tcaatcctcc aacgtggcga aggcgatggt ggggattgat gaatatcggg aagtcccgtt
     3481 tgttctcaaa gggaaatatc ttcaatttct ttccttatcc gatggtgccg ccattcctga
     3541 tgtgggaggg gagttgtcga ttgacggtga tccgacgtct gtcgtgcgaa tgggtttgaa
     3601 aggccatagc ctgagtatcg ggacagacca gggactcgtc tatccggtca acatccttta
     3661 tcgatctgat ttccaagggg acagacggac tattatcccg agcgtaaagg tcaaggaacc
     3721 tgttcgggtg gttcctgacg ggatgggtat tgttcttcat gcccatgtca tgcggagtga
     3781 tgaatttcaa accgtggtgg ctttcaccgg aggcggggag ctctgggtga cccggattga
     3841 ggaaccaggc gatttttcat tttcggatga agtcgttgtg gaaaccaacc ggcttgtcct
     3901 accaccgaag tttcatctga caacgttggc attggatagt gtggggaaac gattggtggc
     3961 cgggacgagg gatggctatc tccttacatg ggaatggcgg ggacagggat ttgatgccat
     4021 gcctcaatcc gtgcctgttg gggcggccgg ggatgccgtg actgttctct catatctgtt
     4081 aggcgaacga acgttggtgg ttggcaccac ttccggaagc gtgagtacgt gggcgttttc
     4141 agctgatcaa tcgggtccat ccggtagatt cctgcgaaaa gtccatactt tcactcccca
     4201 tgccagggcg gtgacgagtc tctcggtgtc ccaacgtgat aaggggtttt taacagccga
     4261 cattcagggg acgatgtttc tacattattc cacgacagga gaaacgattt tggacattcc
     4321 agggaacgga aatgccctcg aatcgttacg ttatgcccca aaggccgatg gtctggtgtg
     4381 gttgggggag gatggcacgc ttcagaccta tgccattgat aatccacatc cggagatcac
     4441 gttcaaatcc ttattttttc ctgtgaccta tgaaggttat gaccatccgg aaatgatttg
     4501 gcaatcttcc agcggatcgg atgagtttga gccgaaattg ggcctcattc cgctcatgtt
     4561 cggcacgttg aaggggacgt tatatgccat ggtgttggcc atacctcttg cggtcatggg
     4621 ggccatttat acggcgatgt tcatgcaccc tcaccttcgc tccgttatta agccgtcctt
     4681 ggaattgatg gcagccctgc cgtcggtggt attgggattt ctcgcgggtc tctggtttgc
     4741 ccctctctta gagaaaattt tccctgcggt ggttgccatt gtgggatttc ttcctgtggt
     4801 gattgcggcc tgttgtctcg cctggcaatt cctcccaatt cgggtgagac gattggaggt
     4861 gtatggactt gatctgatgg tcatgatgat tgccattgtt ctcaccgtgg gtgcctgttt
     4921 gatagccaat caggcgattg agggttggat atttggagga aactttaaac agtggttggc
     4981 gcagaatttg ggcttaacct atgatcagcg aaatgccatt gtcattagtt ttgcgatggg
     5041 ttttgcggtg attccgatta ttttcagtat tgctgaagat gccattgcca atgtcccccg
     5101 acaacttgtt gccggatcgc tggccctggg agcgacgcgg tggcaaacct taacccggtt
     5161 agtccttatt tcggccagtc cgggcatctt ctcggccttg atgattggat tcgggcgtgc
     5221 cataggggaa acgatgattg tgctgatggc cacgggaaat actccgatca tggattggag
     5281 tatgttcaat ggatttcgta cgctctcggc caacattgcg gtagaaatgc ctgaagctcc
     5341 tcatggcgga acgttgtatc gtgtgttatt tctgtccggt ctgattttgt ttggcttcac
     5401 atttaccatt aatacgattg ctgaaatcgt gcggcaacgg cttcgccaaa aatattctca
     5461 attttagacg agcagccatg aaatttcttc aacgattttt tgcatcaggc acattgttta
     5521 tctggttatc aggaggagcc ttagcctgtt ccctgctgct gatcggcggt cttttgatgc
     5581 tcattttcat caatggtatg ggctattttt ggcagaagga tctcgtccag ttgacgctca
     5641 acgataacac ccgggtcctc ggggaaatcg tagataccca gcgtattccg gatacggtta
     5701 ctgcggattt tcctgatggg cagtctcgca tacagctgaa ggtgggcaac cgggatgtgt
     5761 acggactgga tttccggtgg gttgatgaag ccgacattgc tcaacgggat tggcctcagg
     5821 atgtcatcgc ctttgaacga cgcgaatggg ggaattttta tggttgggtg actgcggtgt
     5881 gggacggtga ctcccgtctg gctggtggcc gggatattgc gtgggaggtc tggtatccct
     5941 tccttgagca agccgagtca ctttaccagg agattcatca tattgaacgc gatgtgattg
     6001 gcgccattaa tgctgacatg gagcgcgcgc gacttagtct gaagaccttg gagttgaagg
     6061 gggacatttc gcaggattct gccgactctc ttcaacggga tatccaagaa cgggaagcgg
     6121 cctatcagga gcagatccaa cgattggtgg ggctgtatga gcgattgaac cgctatcggg
     6181 tcaccctcat ggatgtcact gggaaggaaa aaacatttcc ggttggcggc cttgtgaggg
     6241 cgtggcgtcc gaattccatg agtgtgtggg ataaaaccac gatgtatgtg gaaaattttt
     6301 ggaatgttct gagcgatgaa ccgagggaag cgaataccga aggcggcatc tttccggcca
     6361 tattcggaac ggttatgatg gtgttggtga tgagtgtggt tgtggtcccg tttggagtct
     6421 tggccgccgt gtatttaaaa gaatatgcca aacaaggcct gttgactcga atgatccgga
     6481 ttgcggtgaa taatcttgcg ggggttccgt caattgtctt tggtgtgttt ggtctggggt
     6541 tttttgtcta tgcagtggga ggctcaattg accagttatt cttctccgcc tcgctgccga
     6601 atccgacatt tgggacaggt ggaatgttat gggcttccct caccttggcc ctcatgaccg
     6661 tgccggtggt cattgtggcg accgaagaag gcttgtcggc tgttcccaga gaatatcggg
     6721 aggggtcgtt ggggttgggg gcgacaaagt ttgaatcgct catgcgggtt gttctgcctt
     6781 gcgccatgcc gggaatttta accggtttga ttctcgcggt ctcccgggct acgggggaag
     6841 tagcgccact gatgttaacc ggggtagtca agcttgctcc ggccctgccg cttgatggtc
     6901 aatttccctt tatccatctt gatcgaaaat ttatgcattt gggttttcat atctatgatg
     6961 tgggatttca atcccctaat gtggaggcgg ccaagcccat ggtgtatgtc accaccctgg
     7021 tcctggttct tgtcgttctc ctcctgaata tgacggcgat tgcactccgg acccggatgc
     7081 ggaagcgcta tgctggtgct tcggtttaaa tgaggtacaa tgatggaaat cgatcagaat
     7141 actttggaac aatctatgtc tttagccaaa accacgggtg caaagtccct gaggctggtg
     7201 cttgaaacaa ggcagacgaa tccgatgaag aagacttttg tcgaatctga aaccgtccaa
     7261 catccattga ccgattcccc tgcgaaaatt gtcattcagg acatgaattt ttactacggg
     7321 acggttcagg cgctcaaatc cgtgaatatg accattcctg ctaataaagt caccgcattt
     7381 atcggacctt caggttgtgg aaaatcaacc ctcttgcggt gcctcaatcg cctgaatgat
     7441 ctggtcgatg gcaccagggt cgaaggaacc attctcctgg atgatgaaaa tatttatcga
     7501 cctgaagtcg atgccactga ccttcgaacc cgggttggta tggtgtttca gaaaattaat
     7561 ccgtttccga aaaccgtctg gcagaatgtg gcgtatggtc cgcaactgca gaaaattcgc
     7621 aaacgtgcca ctcttgacgc gatcgttgag accagccttc aaggagcggg attgtgggat
     7681 gaagttaaag atcggttgca tgtcagcgcg ttgggcttgt cgggaggcca acaacaacga
     7741 ctctgtattg cgagagcgct tgcggtcaat ccggaagtgc tgttgatgga tgaaccgtgt
     7801 tcggcccttg atccaatatc caccggcaga atagaagaac tcctccatac gttgaaagag
     7861 cggctcacgg tcgtgattgt gacgcataac atgcagcagg ccgcgcgggt ttccgacttg
     7921 acagggtttt tcttattagg ggagttaatc gaattcgagg acaccaaaaa gatttttacc
     7981 aatcctggtg attcccgaac ggaagattac gtgacagggc ggttcggctg atttccagta
     8041 aaccggaata aaggatcacg atgaagcgac attttgatga ttcgttggcg gatctcaggc
     8101 agagaatttt gcggatgggg gcgctggtgg aagggcagat tcgtcaagcg cttatcgcac
     8161 ttgtggatcg cgatgacctg gtggcaaatc aggtcattca gaatgatcgt caggtgaata
     8221 ccatggatgt ggccattgac gaactctgtc tcgaattgtt ggcactccac cagccggccg
     8281 ctaaagatct ccgtttcgtc acgacggcca tgaagatttc cactgaatta gagcgcatga
     8341 gcgatttggc agaaaatatt tgtgaacgcg cactggaact gcataccgaa cctcaattga
     8401 aaccgtatat cgatattccg ttgatggccg agagggccat caaaatggtg ggtgaagcct
     8461 tggatgcgtt tgtccggggg gattcggttc ttgctagaca ggtcctcaag gaggatgatt
     8521 atgtcgatga actgaatgaa caaattttcc gtgaattgtt gtccttcatg atggaaaatc
     8581 cccaaacgat ttcgcgcggg atccggctgt cgtttatctc gaaatatatt gaacgcattg
     8641 ccgatcatgc aacgaatgtg gcggaactgg tggtgtatat ggtggaagga aaaatcatcc
     8701 gccatatgat tccgacctaa cgcagtcgtc ctgccctcgt ttttttctca ttctcttctc
     8761 cttttgtctt gtttcagctt ccgcgtcttc tgtcacaaaa aatttacctg tctgtgacag
     8821 gggtgtcatg tctctcacgt atggtgtggg tatgaaaaat aatcaccaga caccagcgca
     8881 tgcggaagga gaatgctatg caaaaatggg ttgacacacg cggaggttct tcacagtatt
     8941 ccaaagggca ggcgactcac ttaccttcac agggctcagg ttcactttgg tctctggtct
     9001 tggcaggtgg acatggggag cgccttcggc agtataccga acagtgtttc ggtgcatatc
     9061 gaccgaagca atattgtgcc tttctgggaa agcgatccat gatccagcat acgtggaccc
     9121 gggcgaaagc cctctgcctt cctcatcagg ttgtgacggt gatggatcaa tcccattggg
     9181 gctatgtgcg aacccaattg atccaggaat ccctgggccg cttggtctgg caacccgata
     9241 atcgaggaac cggacccggt gtgtttctgc cgctctccta tatccgagcg catgatcctt
     9301 ctgcaacggt agtgttgtgg ccctctgacc atttcatttt ccccgttgag ccgatgcttc
     9361 aggtgatccg cgatgcggcc gccttggtcc aggaacaccc ttatcgtatg gtgttgttga
     9421 ctgttcctcc tcaatcccga gagcaagact atggttgggt gattcctggt gatcgtattc
     9481 cgcattccgg agggagccgg gtcagggaag tgcggagatt tgtggagaaa cccatgggtt
     9541 tacagcttga ggacattgcc ctgagtgggg ggcaatggaa tacgttagtg ctcatagcca
     9601 atgttcaaac cttatgggag gccggccgtc agtgtatccc ggaagtcgtg atgcctcttg
     9661 aagagcatgc gtgtgggatt ggaacctcaa acgaacagca acggttactg catctctatc
     9721 gaacaatgcc caccagaaat ttctccagtg atctgttggc gctcattcct cagcaattac
     9781 acatgatgga tctgaccgga gtccattgga gtgattgggg aaggccggag cgcattcagg
     9841 aaaccgtcgc gtatatgaga cgacatggat ctattgggac gaggggtggg tcgcctagtc
     9901 ttgtttggat ggagccccgg caggacaagg cggtaccaga ggaattaagc gtactcacga
     9961 gatagagagc ctggtcgttt tattggatta atgggtcgtc ctgattctcg accattagtt
    10021 gcccggtcgc cagaaattct ggttgacccc ggttatcggg ttttgaatca tgtctgtgat
    10081 gaaagagccg cagagaggga tgtaggagcg ctttccttga agaagagcgg gatcgtggcc
    10141 cacatgcatt gtcacagagc agacatcggc ccatccaaat gcggtgtgag agttgtgagc
    10201 gggagtggga gtccgcccat tatcaggagg aatgtcggta gggagttgga aatgttattc
    10261 gtttctgatg gctgattaat gatgcctgat gaagccacca gtagattgta agggagatgt
    10321 ggcggcgtac ctgaaacgaa aaaaccaccg ctcagtcaca gtctttagag tgaaggcggt
    10381 ttggtgtgtt ttcctttcca gtttgcaata aaccaatcct gggcgtgaaa tggtgcttcg
    10441 ttttccaaag gcttaacgcg agtatagctt ccatcggatt ggagttgtcg cgtgtgcatg
    10501 ttgtccttca gatagggacg taagatggtt tcaacaatga aggttcgcag tgtggaattc
    10561 tcaatcggaa acaaaacttc aacccgtcgg tccatattgc ggggcatgag atccgcactt
    10621 cccagaaata attcatcctg ccctccgtta cgaaaataat aacagcgaga atgttctagg
    10681 aatcgaccca cgagagacgt gaccgtaatg gtctggctga gtcccgggat gccggggcgg
    10741 agcccgcaca ttccacggac catgagctca atcctgaccc cagcttgaga ggctcgatac
    10801 aaggcctgaa tagaggcttt gtcaacgagt gaattcacct tgaatgccag gtacccgtct
    10861 ccatgttgtt gttggcggga tatttcccgg ttaattcggt cgatgatatt cgttcgaatt
    10921 cccaccggag caatcaagag tttcgcataa gagtcttttt tggaataccc cgttaaggca
    10981 ttgaacagat cagtgatatc ttccccgaat accggctgac aggtaaataa actaaaatcc
    11041 gtatagaact tgctggtgat ggggttgtaa ttgccggttc caagatgcag atagcggcga
    11101 atgccgtccg attcccggcg aacgaccatg cagaccttgg cgtgggtttt caggcccaga
    11161 acaccataca ccacatgaac cccttcatct tctaattttc ttgcccattc aatattgttt
    11221 tcctcatcga accgggcttt gagttccagg agaacggcaa cttgcttgtc attttgtctg
    11281 gcttccatga gggcgtcgac caccggggag ttaatgccta cccgataaag tgtctgtttg
    11341 atcgctaaca ccttcgggtc cagagcggct tgtcgaatga aatcgacaac aggagtgaag
    11401 ctgtcgtatg ggtgatgaag gaagatgtcc tcgcgtttga tgagtgaaaa cagtgattct
    11461 tttgatgcgg ggtcgtgcag aatattgggg aaatgtggtg gaaatttcag attcggtcga
    11521 tcaatggatg tgagttccag aagattcgag aggccgagac ggaaattata ggtgtagacc
    11581 tgatagggtg ctaaattgag atttctgatc aggatatctt ttatggaatc cggcatatcc
    11641 cggtccagtt ccaatcgcac cacagacccg aactgtcgca aatcaatttg ttcctcaatg
    11701 gcagccagga gatcgccggc ttcatcttct tcgatttcaa aatctgcatc cctggtaatt
    11761 cgaaaaa
//
LOCUS       DAORAQ010000074        11746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_901_length_11746_cov_64.898298, whole genome shotgun sequence.
ACCESSION   DAORAQ010000074 DAORAQ010000000
VERSION     DAORAQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_11746_cov_64.898298"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKM72_08245"
     CDS             complement(<1..213)
                     /locus_tag="PKM72_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60813.1"
                     /translation="MELCQNLFANIPEHLNHELTSEVLRSRSVRIERIVSRGQASPPD
                     FWYDQAEHECVVVLAGKATLKIEGQPE"
     gene            complement(539..1483)
                     /locus_tag="PKM72_08250"
     CDS             complement(539..1483)
                     /locus_tag="PKM72_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNP60814.1"
                     /translation="MTEVLFFSKVRSVWKFGLLGGCLVAIMAGCSSKLQTTVISVTEP
                     PAKSQVVKLEPVASQAEAVVQPPVKAETFIGIPAMDIPVEEPARPFIRPSAPADIFAT
                     PRTTVVEPAVPEALEAPLVAESVVPPSPIAPTPNQISPQELSQEGGVPPILSEPETPA
                     RPTIRGDAVPPEQLIARVEPEEDKPVQQVAELPEIFEEKTEDLLKSLGDIYFDYDRFS
                     IRKDAISVLLENAQTLASGLSNNKIVIEGHCDQRGTESYNMVLGERRANAVREYLVDL
                     GVPSENLQVVSYGKEKPFCTDQNEKCWQENRRSHFVVQ"
     gene            2795..3268
                     /locus_tag="PKM72_08255"
     CDS             2795..3268
                     /locus_tag="PKM72_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60815.1"
                     /translation="MKILGVYQHSPKPDPPKVPIKDRILLVDDDRNHRTCLKEFLELR
                     GYLCSEAGNGLEGLETLQKEAVSIIITDNNMPQMDGLDFIEQVNHKYSQEILSIFLIT
                     AELSHIVRLRAFKNGVNRVFEKPLDFQELCNAVDWVTKFDIHQSTTNKYSSARYL"
     gene            3501..3671
                     /locus_tag="PKM72_08260"
     CDS             3501..3671
                     /locus_tag="PKM72_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60816.1"
                     /translation="MSDSQTEYQLRDRVSCMRLFSLDRAQGIPDAKALGLVREPLAAA
                     DVVDALVEPWDP"
     gene            3797..4411
                     /locus_tag="PKM72_08265"
     CDS             3797..4411
                     /locus_tag="PKM72_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60817.1"
                     /translation="MGSPPGPTTAERCRCPLVKHAGGPSLGLEESGEYRQAAQADSAR
                     DRNASHRAGQPRPGGRVAPRARRTGRGGGFAYSSQETTPRTSAPISRKTAIGTSLCRR
                     SHRTGIGVERRRAFASNRSSVTTCPRWETAHSSEWVGRARAKIGRKNQGDNVPRVPFL
                     TAASLQQGAAGKTLNEPQSTTPSHAHDAQGLPPNTQAGQGREVP"
     gene            complement(4565..7975)
                     /locus_tag="PKM72_08270"
     CDS             complement(4565..7975)
                     /locus_tag="PKM72_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP60818.1"
                     /translation="MESPHPSKSSPPSDKEVHREHLGDLTLSLGSSQGSSGQLSSESS
                     SHHYRVRESPQSNLSPGTPHSDSHEELNALRQQVERFIFASQGSREGLWVAEICPDIP
                     WYSPDCPVWYSPQFIAMLGYEESEFPGVRGSWEQRLHPDDRERVFAALTNHIEKRIPY
                     HVESRLRTKANGYRWFEATGEGTFDADGKLLRGGGTIRDITERKLADDISRRNHALLD
                     AVLEEMSDVVYVKDQDGRYLLVNPSGAEIMGKAIHEVIGKTDQEIFGNHSPALFVQGD
                     AQVMGSAGTQTFEVEVQEQHPVSRTFLVTKDSFGSLTEGQNGVLGFARDITFRKAAEL
                     TIQEREKRFRAIMENAYDLITEADSEGRFLYVSPNFKESLGYSSQDLLGTSVFAPVHP
                     EDREMVEAEFCQGMKTQGSGRSVYRYRHQNGEYRWFESTGRAFHTALGELRAVVISRD
                     ITQRKQWEDALESIVKGNVLPGSPDFFEVLVVELAKALQVSMVFLSERIEKNTSKART
                     LAFWNQDHFEPSTVYECMGGPCELVLGGDPVGHPSGVQQLFPHSEAIQALKVDAYYGT
                     PLFNSKNEVVGNLALLDTQQFVLSSQGQNLLKIFAARAGAELERKRAQEEVQESQDQY
                     RELYDQTPLIYFTVNSQGLVLSVNQYGAQLLGYQVEELLGTSAFSVVHEDDRDLFKSG
                     LEKGFRESTKGVLPEFRKVKKDGTILWVKETIQAIEKQSGPRVWLLSCEDITERKRTE
                     EALVLSETQLRHTQKMEAIGTLAGGIAHDFNNILGAILGYSELAMAYATKDERLKSYL
                     TEVVAAGNRARDLVKQILAFSRRSEKDKEAIDLRLVINDVLQMLRASFPSSIEIRTSL
                     DLESTVIYGDRTQIQQVIMNLCANAEYAMREEGGLLEIGLCHEGIPEEGIPGMGMLKV
                     GSYLQLVIRDTGKGIPLEMVERIFEPFFTTKPAGEGTGLGLAVVHGIVHNHGGEIAVS
                     SPPGGGTTFTILLPRLDVVVPVKPEEVMAWPTGSGRILFVDDEEMLTRWGTQFLSHLG
                     YSVVASVNPYEALDLFRAHPWDFDVVVTDQTMPTMSGEALSRALLGIRADIPILLCTG
                     FSHTMTQEKAEQLGIQAFLMKPVNGLSLAIALRDILDGGPTPHSSQHQ"
     gene            8218..9009
                     /gene="thiD"
                     /locus_tag="PKM72_08275"
     CDS             8218..9009
                     /gene="thiD"
                     /locus_tag="PKM72_08275"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNP60819.1"
                     /translation="MKKAVLTIAGSDSSGGAGIQADLKAMSANGVFGMSVLTSITAQN
                     TCGVSAVFHLPSSIIESQLDALFSDIPITIAKTGMLATSDIITTVSRNLAKQHLEHLV
                     VDPVMVAKGGYPLLEQDAMATLKTALLPQASLLTPNIAEAELLSGLSIKTLANAREAA
                     KVIHQFGCKHVLIKGGHLLEQPGTDLLYDGRFFRMFKGEFLQTKTTHGTGCTLASAIA
                     ANLAKGKTIPDAIEAAKHYTTEAIRHGLALGQGNGPTDHFYFLQS"
     gene            9018..9827
                     /locus_tag="PKM72_08280"
     CDS             9018..9827
                     /locus_tag="PKM72_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNP60820.1"
                     /translation="MEIASPTESGDGGTETPVNVTGPSGIVTTGIFAQPAAPTNRAVI
                     LCHGFPSDKNSRTNRRLTELLIPQSIATLRFDWYGMGDSPEPLTTFSIKKCEDQLDAA
                     VHFLTERSMKALGLIGSSFGGFMTILSASRYPQLQALGLKCPVVDFSEVLRLELGADA
                     MNQWKITDHIPNVFGGDLPTRLPYAFFEECLTYNGYHSASCIQAPTVIVHGDQDEIIP
                     AHQIDRLLTSLNVSKDFRLIPGANHQFGRPEDFRLMTNHLAKWMIAYLPPS"
     gene            9846..10322
                     /locus_tag="PKM72_08285"
     CDS             9846..10322
                     /locus_tag="PKM72_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60821.1"
                     /translation="MPDPPNTNKELPKEQLEVFRVLYPLFKNEVLRRREYMSRLSAFY
                     NTVLVLLIMTIAVISPGHPDLAMRWLAILGVGILSGFVAYLILQQATRHRMAKQQLIE
                     LERGIGLYQEGWISSGKTAYPQHWQTDWLSDRSVATYLTILAVLSGLVICAILIRL"
     gene            10378..10896
                     /locus_tag="PKM72_08290"
     CDS             10378..10896
                     /locus_tag="PKM72_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNP60822.1"
                     /translation="MLKTYRGIAPTIAPSAFIEDTAIVIGDVAIGSESSVWFNAVIRG
                     DVHSIRIGHRTNIQDLCLLHVTHDTHPLTLGDDITVGHHVVLHGCTIHDRVLIGMGTI
                     IMDGVVIEEDCVIGAGALITGNIHIPSRSLVMGSPAKVKRMLTDAEVQWIKESAGNYI
                     RYAQQYVEEQNG"
     gene            10987..>11746
                     /locus_tag="PKM72_08295"
     CDS             10987..>11746
                     /locus_tag="PKM72_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60823.1"
                     /translation="MSRSSLQHIPLVILGTGYTGRRLYHQAVNAGWNTFSTSRMPDVH
                     LSDIPPESRIAFDLTQTETWRFIPSPAHLIWCFPATPLSLVQAFFATRPMDSGRILVM
                     GSTSAYGTNCGIVTEQTPVNLDLPRVQGEEYLRTQLGAVIIRLAGLYGPGRHVLNWMR
                     NGRIHNTPKWVNLLHVEDAAGICLRALERAQKGTTYLASDGTPRTWTEIFSIASGKWS
                     MPIPPPTPHREIGKQVSIHKLLTTLHYTLRFPDLY"
BASE COUNT         3036 a         3273 c         2613 g         2824 t
ORIGIN      
        1 ttcaggttgt ccctcaattt tgagtgtggc ttttcctgcc agtaccacaa cacattcatg
       61 ctcggcctgg tcataccaaa agtctggtgg agacgcctgc ccccgtgaca ctatccgttc
      121 aattcgtaca gaacgactcc tcaatacctc actcgtcaat tcgtgattaa ggtgttcagg
      181 aatattggcg aacagatttt gacataattc cattgcctcc acctctctag aaaaggtcac
      241 ttgctttatc gcattctcaa ttttagtact ggttaactcc catgggcgca tcgaattggg
      301 aattgacaat ggcggaaaaa tcgggggtca agcagatctg gagggaaata ctgaagaatg
      361 ttcggcttgg ctctgagctt tgaaagacca agcagaagaa tcagaaagac tgccgataat
      421 tcaaaaacga caattaagga acgggccagc catccccctc aggaagaatg gcccgctact
      481 ttctccattc aagacgctcc tccaagaaac gtctctagcc caatcaattt aatcctcttt
      541 actgtacgac aaaatgacta cgccggtttt cctgccagca cttttcattc tgatcggtgc
      601 aaaacggctt ctcttttcca taactcacaa cctgaagatt ctcactagga acgcccaaat
      661 caactaaata ttccctcacc gcatttgctc ttcgctcacc aagcaccatg ttgtaacttt
      721 ccgtaccacg ctgatcacaa tgtccttcga tcacaatctt gttattcgac aaacccgaag
      781 ccagtgtttg ggcattttct aacagaactg aaatagcatc cttccgaata gaaaatcggt
      841 cataatcgaa ataaatatct cccagagact tgagaagatc ttcagtcttc tcctcaaaaa
      901 tttctggcag ttctgcaacc tgctgcaccg gtttgtcctc ctcgggttcc acccgagcga
      961 tcaattgttc cgggggaacc gcgtctccac gaattgtcgg gcgtgccggt gtttccggtt
     1021 cggacaaaat cggcgggact ccaccctctt gtgataattc ttgaggagaa atttggttcg
     1081 gggtgggagc tataggagac ggaggaacca cagattcagc caccagggga gcttcaagag
     1141 cttcaggaac agccggttcg acgactgtcg ttcgaggagt ggcaaaaata tcagcaggag
     1201 ccgaaggacg aataaatgga cgagcaggct cttcaacggg aatatccata gccggaatcc
     1261 caatgaatgt ttcagccttt acgggtggct gcacaaccgc ctcagcctgc gacgccacag
     1321 gctcaagttt taccacttga gacttggccg gaggttcagt cacacttata accgtcgttt
     1381 gcaatttgga actacacccc gccattattg caaccaagca tccgcccagt aggccaaatt
     1441 tccagacact ccttacctta gaaaaaaaca atacttcggt catgttttac ctccactatt
     1501 cctttccttg actgacggat gcatccacat agaccccatc caatatgatg attctttttc
     1561 ccattcttat ttcctctaca ataatttctt taagaaaatg acagtgtcat tttcaataac
     1621 aaagtaatct caaaactgta tgcagcccct tcgaatgatg tcaagaaccc tggcgtggaa
     1681 cttgaggtcc actcctgatt tattccctaa gaatgccttt tggtaggatc caggtcaagg
     1741 gtgataggaa aaaactacat gcccccccac atggaacaac cgctatccct gatctttaca
     1801 cttcccaatg gggcaagcct agccctcctc aacatcctta attgtgacat catagtcgcc
     1861 tgccatctcc cccaacatag atatttcttt cctactaaca tcagaattct catcattgtc
     1921 ccaagtcgct tccgtctgga tttagccaga taagagtatt caccaactat tagacatcgc
     1981 ggaccaacag gagcaggacc gctgactctg gaactccttt tgttctcgaa agtttctcaa
     2041 gacagccact cacaatgatc ctcagatatg gcaactcaaa ccttttccta atggctattt
     2101 cgaaacatta cccgaactaa tacacaccaa ttcatgtgaa gtttcttaga gggtccggcc
     2161 caatgtcgaa ttatttcgag atctaacaat tcatataaaa aaatactggc actttccgtt
     2221 tcacgaatca tttctcgtcg tgatttaaac atccatttct ctattcctgg cgttcgtggg
     2281 taagcccttc ttaagcaaaa gccgccccgt caaatgcttt cccaagttaa tcaattgaag
     2341 ctgtccgacc atttgactca tgaagggagt ccgctcggtg ctgtagggtt tcgcctgcat
     2401 agtttttggc aaaacttttg tccccacacc tcataaccat ttgaaatttt taattttttt
     2461 tataataaca tttcttggaa caccccttgc acttttacca atcaactatt actgcacacc
     2521 agttttttgg aggcgaagcc atttttttaa atattccgct tttacgacct aatagcatgg
     2581 agaatgacgc aattcgactt tcataaccac caacttgaca catgataata ctggttttaa
     2641 gcgcctcgat atgagcctcg gcggagggac tggtttcagg ggaactacga tttcgaccgc
     2701 acccggagtc tggtctcctc atcagccacc ttctcaaagg tccctccgcc acctttttag
     2761 aaaacaaatc tgaccccaat aaatttaaaa catcatgaaa atcttaggcg tataccagca
     2821 ttcaccaaaa cccgatccac ccaaggttcc cattaaagac cgaatcttgt tagttgacga
     2881 tgatcgaaat catcgtactt gcctgaagga atttttagaa cttcgggggt atttgtgttc
     2941 agaagcagga aatggcctgg aagggcttga aactttgcaa aaagaggcgg tctccatcat
     3001 cattaccgac aacaacatgc cacagatgga tgggcttgat tttattgaac aagtcaatca
     3061 taaatattcc caggaaattc tctcaatttt tctgattact gctgaattat cccacattgt
     3121 ccgtcttcgc gcatttaaaa acggcgtcaa tcgggtcttt gaaaaacccc tagactttca
     3181 agagctttgt aatgccgtgg actgggtcac gaagtttgac atccatcaat cgacaaccaa
     3241 caaatactca tccgcaagat acctctaaga atatttcctc catcctcaat agagggaatc
     3301 tctaaaaatt cctggcaaag ggctttaagg atgccatgcg gcctgtccaa ggaggactta
     3361 ccgctgtcga ccctgcggcc acttggattc gttgatatta cgacacgcta gtaggccctc
     3421 aggcactggg gcgatccctt agctcaccat acgcaggtcg ggaagccctt tgaccggcct
     3481 tcggcaacgc cctttagaat gtgtccgact cccaaacgga atatcagctc cgcgatcgtg
     3541 tgtcgtgtat gcgcctcttc tccctcgatc gggcccaagg cattcccgat gcgaaggctc
     3601 tcggactggt tcgagaaccc ttggcagcag cggacgtggt cgacgccctg gtcgagccat
     3661 gggaccccta gctggacgat cagggactcc aaccgcgagg ggaccaacgg atcgatgcgt
     3721 cctgggtccc ggttccgcag caagggcata gccgagaaga aactgcggcg ctgaaagcgg
     3781 gcagaagcgc cacagcgtgg gaagcccacc cggccccacg acggcagaac gatgtcgatg
     3841 cccgttggtt aagcacgcag ggggaccatc actcgggcta gaagaatcag gtgaatatcg
     3901 acaggccgca caagcggatt cggcgagaga ccgtaacgcc agccaccgtg caggacagcc
     3961 acgcccggga ggccgggtag cccccagagc caggcgtacc ggtcgtgggg gcggattcgc
     4021 ctatagctca caggagacaa cacccaggac ctccgctccc ataagcagga aaacggctat
     4081 cgggacaagc ctctgtcggc gcagccacag gaccggaatc ggcgtcgagc gcagacgcgc
     4141 gttcgcgtcg aataggtctt cagtcaccac atgtccgcga tgggagacgg ctcattcgag
     4201 cgagtgggtg gggcgagctc gagcaaagat cgggcgtaag aatcagggtg ataacgtccc
     4261 acgggtgccg tttctcacag ccgccagtct gcagcagggg gcagcgggaa agaccttgaa
     4321 cgaaccccag tcaaccacgc cctctcacgc acacgatgct cagggccttc cgcccaatac
     4381 tcaagcaggc cagggaaggg aggtcccata actacgggtt ttaagaggtg ccctagacat
     4441 attgcctgca tgataaaacc tacttcctgg gatcgacctc gaagaatgtg tcagagcttc
     4501 gtcatatcat cattgaccga aacgggataa tcagtaaagt tccccgccca aagggcaggg
     4561 aactttactg atgttggctc gagtgcggag tgggacctcc gtccaggatg tcccggagcg
     4621 caatagccag agacagtccg ttgaccggct tcattaagaa ggcctgaatg ccaagttgct
     4681 cggctttttc ttgagtcatg gtgtggctaa accccgtaca caacagaata ggaatatccg
     4741 ctcgaatacc taacaatgca cgagataagg cctcaccact catcgtcggc atggtttggt
     4801 ctgtcacgac cacgtcaaaa tcccacggat gtgctcggaa aagatctaac gcttcatacg
     4861 gattgacgct tgccacaacg gaatagccta aatggctgag aaattgcgtc ccccagcgcg
     4921 tcagcatttc ttcatcatcc acaaataata tccttcccga tccagtcggc caggccatca
     4981 cttcctcggg ttttacgggc accaccacat ccaggcgcgg caggagtatc gtgaaggtag
     5041 ttccccctcc tggaggactg gacacggcaa tctcaccacc atggttatgc acgataccat
     5101 gcacgacggc cagcccgagt cccgtgcctt ccccagcagg ttttgtagta aagaatggct
     5161 caaatattct ctcgaccatt tcgaggggaa tgccctttcc tgtgtcacga atcacaagct
     5221 gaagatagga tcctacctta agcataccca tgcccggtat cccttcctcg ggaataccct
     5281 catgacataa gccaatttcc aacaaacctc cctcttcccg catggcatat tccgcgttgg
     5341 cacacagatt cataatcacc tgctggattt gggtacggtc accataaata accgtggact
     5401 caagatctaa tgacgttcga atttcaattg aggaaggaaa gctggcacgc agcatttgca
     5461 ggacatcatt gatcaccaat cgcaggtcaa tcgcttcttt atctttttcc gaccgacggc
     5521 taaaggctaa aatttgcttc accaaatctc gggcccgatt cccggcggca accacctcag
     5581 ttaaatacga tttcagacgt tcatccttgg tagcataagc catggccaac tcagaatatc
     5641 ccaatatcgc acccaaaata ttattaaaat catgggcaat accaccggcc aaggtaccga
     5701 tcgcctccat tttttgagta tgccgcaatt gggtctcact caataccaaa gcctcctcgg
     5761 tccgtttccg ctctgtaata tcctcacaag atagtaacca cacacgaggc ccggattgtt
     5821 tctcaatggc ctgaattgtt tctttcaccc aaaggatggt tccatctttt tttaccttcc
     5881 gaaattcagg aagaacgcct ttggtcgatt cacgaaatcc cttctccagc cctgatttaa
     5941 ataagtcacg atcatcttca tggaccacgg agaacgcaga tgtccccaat aactcctcaa
     6001 cctggtaccc taacaattga gccccatact ggttaacgga gaggacgaga ccctgactat
     6061 tcaccgtaaa atatattaat ggggtctgat cgtagagctc acgatactgg tcctggcttt
     6121 cctggacctc ttcctgtgcc cgcttccgct caagttcagc tcccgctcgt gccgcaaaaa
     6181 ttttcaaaag attttgccct tgagaggaaa gaacaaattg ttgggtatcc aggagcgcca
     6241 gatttcccac aacctcattc ttggaattaa aaagaggagt tccataatac gcatccactt
     6301 tcaaggcttg aatcgcttca ctgtgtggaa agagttgttg aactccggag ggatggccaa
     6361 cagggtcacc cccaaggacg agttcgcaag gcccccccat acattcatac accgttgacg
     6421 gctcaaaatg gtcttggttc caaaaagcca atgtccgggc tttgctggta tttttctcaa
     6481 tccgctccga caaaaacacc atggacacct gaagagcttt cgccaattcg acaactagaa
     6541 cttcgaagaa atctggagaa ccgggaagca cattgccttt gacaatcgat tccagggcat
     6601 cttcccattg ttttcgttgc gtaatatccc gtgaaatgac gacagctcga agttcaccaa
     6661 gcgccgtatg aaaagctcgc cccgtgcttt caaaccaacg gtactctcca ttctgatgtc
     6721 gatagcgata caccgaccga ccggaacctt gtgtcttcat gccttgacaa aactctgctt
     6781 ccaccatttc tcgatcttcg ggatgaactg gggcaaagac acttgtgcca agcagatctt
     6841 gggagctgta ccccagagat tctttaaaat tagggctcac atacaggaac cgtccttccg
     6901 aatcggcttc cgtgatcaag tcataggcat tttccataat ggcccgaaag cgtttttccc
     6961 gttcctgaat ggttagctca gcggcttttc tgaacgtaat atcacgagca aaacccaaga
     7021 ccccattctg accctccgtt aaggatccaa aggagtcttt cgtgactaaa aacgttcggg
     7081 acacgggatg ctgctcctgt acctctactt caaatgtctg agtcccggca gatcccatca
     7141 cctgggcatc accctgaaca aacaacgccg gcgaatggtt cccaaatatt tcctggtcgg
     7201 tttttccgat gacctcatgt atggcctttc ccatgatctc cgctcccgaa ggattcacca
     7261 ggaggtaacg accatcctgg tccttgacgt acaccacatc actcatttct tcaaggaccg
     7321 catctaacag agcgtggttt cgccttgaaa tgtcatcggc caacttacgc tccgtaatat
     7381 cgcgtatggt tcctccacct cgaagtaact tgccgtcggc atcaaaggtg ccctcgccgg
     7441 tggcctcaaa ccaacgataa ccattggctt tagtgcgtaa tctggactcc acatggtacg
     7501 gaatcctctt ttcaatatga ttggttaaag ccgcaaacac ccgctcccga tcgtcaggat
     7561 gtaaccgttg ctcccaactt ccccgcactc ctgggaactc agattcctca taccccaaca
     7621 tcgcgataaa ctgaggggaa taccacaccg gacaatctgg agaataccag ggaatatccg
     7681 gacaaatttc agccacccat aacccctcac gagagccctg agaggcaaaa atgaaccgct
     7741 ccacctgctg acggagagca ttcaattctt catgtgaatc agaatgtggg gtacctggcg
     7801 acaggttgga ttggggggac tctcgaactc ggtaatggtg gctcgaagat tccgaagata
     7861 attgtccaga tgacccttgg gaagacccaa gggaaagcgt cagatctccc aagtgctccc
     7921 gatgcacttc tttatcggaa ggaggagaag actttgaagg atggggagat tccatacagc
     7981 atctatcctt gaaagcccgg caaggccaga ccatcaagga gtgactctat aatcaaacac
     8041 catgaaatat tttttctata atgtcaagaa aaaatactca aaaacaccac acactcagac
     8101 gtaagatgga acagcagggt cttcaaccca aataaaataa ggaggcttca tccctggatc
     8161 atcagaatat gacatgctag actcgctgtt cgtccacacg ctcattcgct gacacatatg
     8221 aaaaaagctg ttctcaccat cgccggatct gattccagtg gaggagccgg aatccaagcc
     8281 gacctcaaag ccatgtccgc caatggagtg ttcggcatgt cggtgctgac atccataacc
     8341 gcacaaaaca cgtgcggcgt ctctgccgtt ttccatctgc cttcttcgat tattgagtct
     8401 caactggatg cactcttctc cgacattccg atcacgatcg ctaaaaccgg catgctggcg
     8461 acctccgata tcatcaccac agtcagtcgg aatctggcca agcaacatct tgaacacctg
     8521 gttgtcgacc cggttatggt tgcaaaagga ggatacccac tgctggaaca ggatgcgatg
     8581 gcaacactga aaacggcatt gcttcctcag gcctctcttc tcaccccaaa tattgccgaa
     8641 gccgaattgt tatccggcct ctccattaaa accctggcca atgccagaga ggcggcaaaa
     8701 gtaatccatc aattcggctg caagcatgtt ttaatcaagg gcgggcacct gctggaacag
     8761 ccggggaccg atttactcta tgacggccgg ttttttcgca tgtttaaggg tgaattcctt
     8821 cagacgaaaa cgacccatgg aaccggctgc accctggcct ctgccattgc tgccaacctc
     8881 gccaagggga aaaccattcc cgacgcaatt gaggctgcca aacactatac caccgaggcc
     8941 attcgtcacg gtctagccct tggccagggg aatggcccta ccgaccactt ctactttctt
     9001 caatcatagc gcaattcgtg gagatcgcat cgcccacaga aagcggggac ggggggactg
     9061 aaacccctgt caacgttacc ggtccatccg gcattgtgac gacagggatt ttcgctcagc
     9121 ctgctgctcc cacgaatcga gcggtaatcc tttgtcatgg ctttccctcc gataagaaca
     9181 gtcgaaccaa tcggcgactg acggaacttc tcattcctca atccatcgca actcttcgtt
     9241 ttgattggta tggcatgggt gactcaccgg aaccattgac aaccttcagc atcaaaaaat
     9301 gtgaggatca actggatgcc gccgttcact ttctcaccga acgatccatg aaggctctcg
     9361 gactgattgg atccagtttt ggtggattca tgaccattct ttcagcttcc cggtatcccc
     9421 aactacaggc ccttggcctg aaatgcccgg tggttgattt ttctgaagtg cttcgcctgg
     9481 aattgggggc ggacgccatg aaccaatgga aaatcaccga tcatataccc aacgtctttg
     9541 ggggcgacct tccaacacgg ttgccgtatg catttttcga ggaatgcctc acctacaacg
     9601 gctaccactc tgcttcgtgt attcaagccc ccacagtgat tgtgcatggt gatcaagacg
     9661 aaatcattcc cgctcatcag attgatcgct tactgacttc tctcaacgtg tcaaaggatt
     9721 ttcggctcat tcccggtgcg aaccaccaat ttggaagacc tgaagatttt cgtctgatga
     9781 ccaaccatct ggccaagtgg atgatcgctt atttgcctcc ctcatgatgg gaaaagaccg
     9841 gtttcatgcc tgatccacca aataccaaca aggaattacc caaagagcaa ctcgaagtct
     9901 ttcgcgtgct atatccgtta tttaaaaacg aggtgctccg gcggcgggaa tacatgagcc
     9961 ggttgtccgc attctacaat acggtattag tgctgctgat tatgaccata gcggtgattt
    10021 cccccggaca cccagacttg gccatgcgtt ggttggccat tttgggggtg ggcatactgt
    10081 cagggtttgt ggcctatctg attcttcaac aagccactcg acaccgaatg gccaaacaac
    10141 agttaattga actcgagagg ggaatcggac tgtaccagga aggctggatt tcttcgggga
    10201 aaaccgccta tcctcaacat tggcaaaccg actggctctc ggatcgaagt gtggcgacgt
    10261 acctcaccat cctcgcggtt ctctcaggcc tggttatctg cgccatactc attcgactct
    10321 agccttcacc aaacgtcctc acacggagaa accatattcg gcgaaaggac cgccctcatg
    10381 ttaaaaactt atcgaggcat tgccccaacc atcgctccca gcgcgtttat cgaagacacg
    10441 gccatcgtca tcggcgatgt ggccatcgga tcggaatcga gcgtctggtt caatgccgtt
    10501 atacgaggag acgtacatag tatccgcatt ggccatcgga ccaatattca agacctctgt
    10561 ctgttgcacg tcacacatga tacccacccc ctcactcttg gcgatgacat caccgtcggt
    10621 catcatgtgg tgctccatgg atgcacgatt catgatcggg tattaatcgg gatggggacc
    10681 atcatcatgg atggcgtcgt catcgaagaa gactgtgtca ttggcgctgg ggccttaatc
    10741 accgggaata tccacatccc ctcgagaagc ctggtcatgg ggtctccagc caaagtgaaa
    10801 cgcatgttga cggatgccga agtccagtgg atcaaagaat ccgccgggaa ctacattcga
    10861 tacgcgcaac aatatgtcga agaacagaat gggtaatatc ggaattccat caagctccaa
    10921 ccacggtatg ccctggctct ggaaagattc ctcttcctaa ccacacactt cgacaagacc
    10981 ttttccgtga gccgctcttc tctccaacac attccgctgg tcattctggg aaccggctat
    11041 accggtcgac ggctctatca ccaggcagtc aatgcaggat ggaacacctt ctcgacaagc
    11101 cgtatgcctg atgttcatct ctcagatatt ccgcctgaat ctagaattgc ttttgatctg
    11161 acccaaactg aaacctggcg attcattccc agccccgccc atctcatctg gtgttttccg
    11221 gcgacgccat tgagcctggt gcaagccttc ttcgccacac gccccatgga ttccggcaga
    11281 attctggtca tgggcagcac atcggcctat ggcacaaact gcggaatcgt gaccgaacag
    11341 actcctgtga acctggacct tccccgggtt cagggagaag aatatcttcg gacacaattg
    11401 ggcgccgtca tcattcggtt ggcaggacta tacgggccag gtcgtcatgt gttgaattgg
    11461 atgagaaacg ggaggattca caatactccc aagtgggtca atctcctcca tgtggaagat
    11521 gctgcaggaa tctgtttgcg agcccttgaa cgggctcaga aaggtaccac atatttggct
    11581 agcgatggga ccccacggac ctggacagag attttctcaa ttgcgtcagg aaagtggagc
    11641 atgcctattc ccccacccac gccccatcga gaaatcggca aacaggtctc cattcacaaa
    11701 ctcctcacca cccttcacta tacccttcga tttcctgacc tctatc
//
LOCUS       DAORAQ010000075        11660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_915_length_11660_cov_61.619044, whole genome shotgun sequence.
ACCESSION   DAORAQ010000075 DAORAQ010000000
VERSION     DAORAQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_915_length_11660_cov_61.619044"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PKM72_08300"
     CDS             <1..815
                     /locus_tag="PKM72_08300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNP60824.1"
                     /translation="VVLDEADRMLDMGFAPQLNQIIEALPESRQTLLFSATMPNNLAD
                     LARMSLNDPFKALVAKSATPAEGVTHTVHHTANSDKTSLLLSILKETEGSVLVFTRTK
                     HRADRIGETLEKVGCRVAVLHAGRRLPQRRAALEGFRRGRFEILVATDIAARGLDVDD
                     IQHVINYDLPHVPEDYIHRIGRTARKNTKGWATSFVTGEDNRSLRMIEKLLGKAVPCA
                     PGSRPPMPPVAQERRGPRPNSGGRGFGAPRSGSPARHDRPAGRRPRPSTLAS"
     gene            1380..1781
                     /gene="smbP"
                     /locus_tag="PKM72_08305"
     CDS             1380..1781
                     /gene="smbP"
                     /locus_tag="PKM72_08305"
                     /inference="COORDINATES: protein motif:HMM:NF028095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNP60825.1"
                     /translation="MLSWIPRLTIALLCMGLMAGCASYSTGTVNPHVAEALTHAQEAV
                     DHGGMGHADVVVTHAEASLQHAQAAKKDMNNPQLDAAIAELGEAIAHGEAGHADVATS
                     HAKSAVIHLNQINGMTPSTESNEMVPSSGYY"
     gene            complement(1885..2424)
                     /locus_tag="PKM72_08310"
     CDS             complement(1885..2424)
                     /locus_tag="PKM72_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNP60826.1"
                     /translation="MALHVLARIVCSFLLVASAMATSAQADSQEGGSPMTVSEGKSVS
                     MEYTLTLENKEVLDTNVGGEPLTFTQGSHQIIPGLETALDGMKAGESKQVTVAPEEGY
                     GPINPQAVQEVPIDQIPTDARKVGVKLQGKDGQGRMVTPTVTEVKEQVVVLDFNHPLA
                     GKTLYFDVKILDVKTATAP"
     gene            2823..3467
                     /locus_tag="PKM72_08315"
     CDS             2823..3467
                     /locus_tag="PKM72_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HNP60827.1"
                     /translation="MEPNERMVKAKQQVAQHRREFSCREVDEPQDERVPPGQHVVNNF
                     PVLDLGCKPEIVLAEWALSIDGCVANPLTWDWEAFQRQPQVQLTADFHCVTSWSMLDN
                     EWGGVKFQHLLELVRPLPEAKYVLFEAFDEYTTNVTLKVCNDDDVLLATHWSGRPLTR
                     DHGGPVRVIIPKLYGWKGAKWVKKITFSERDERGFWEVRGYSNTALPWNDDRYG"
     gene            3535..4647
                     /locus_tag="PKM72_08320"
     CDS             3535..4647
                     /locus_tag="PKM72_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNP60828.1"
                     /translation="MPVRDPFCITKIEAWPIALPLLAPFVVASGAMTEAHNVFVRVTL
                     RNGAYGFGEMAPFPDISGEDQAGCLQVFPLAARECLGQSVTHFRKLAQQLREVARRTP
                     AVRCGLETALLDALCRGMSIPLWGLWGGADVRRQETDVTLPIGPLDQMVGTAQAWFAR
                     GFRVFKMKVGLEVDEDIRRMEAVSAACPDSKLVLDANQGFSLEQASECIRAIERLHLP
                     VMLFEQPVAREDVEGFVALRRKGTIPLAADESVRSVEDARGLIERNAVDVLNLKITKC
                     GVVESMDIAGLARASGLGLMIGGMVESRVAMGCSWSLVLGLGGFEFLDLDMPLLLSGD
                     PIQGGYQYEGPVLHPWEESGLGMEMRDEPSSVIVVE"
     gene            4950..6509
                     /locus_tag="PKM72_08325"
     CDS             4950..6509
                     /locus_tag="PKM72_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNP60829.1"
                     /translation="MGIGNRMMGWSKTLGLVGLAWFVSGGFVFGQDLASFEQRVTQHT
                     LKNGWTFIIVERPVAPVFAFMTRVNVGSAQEIMGQTGLAHMFEHMAFKGTPHIGTTDY
                     DAEKIALEALEEAYQAYQTEKFSSDPDPERVDRLYAVFKKRQQEASAYVVKNEFGDII
                     EREGGVALNAFTGADVTGYFYALPANKVELFAYLESERFLHPVFREFYEERDVVMEER
                     RMRTESQPFGRLLEQFVATAYMAHPYHHPVIGFASDIQSYTMTDAKKFYEAQYVPTNL
                     VTAIVGDVKAETVIPILEKYFNRIPSAPSPPPLRIVEPPSIAEKIVKLQDPSQPLYVE
                     GYHKPPVTHTDQPVYDAIDDILTNGRTSRLYRSLIRDKRIAVSVGAYGEYPGDKYPHV
                     WMAYAVPGRGVTNQAVQQALREEFDRLKTQDVSDEELARFRTRAKAGLVRALNNNLGM
                     AMYLTDYQMLFGDWRELFRSIDRLDQVTKSEIRRVANTMFQSTNRIVGMIETVPPADS
                     PQAVTSGEGQS"
     gene            6506..7993
                     /locus_tag="PKM72_08330"
     CDS             6506..7993
                     /locus_tag="PKM72_08330"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNP60830.1"
                     /translation="MNRIMSQQSAVCGVGRWSILSAGLVVFVMFCVPVWVQAQVTKVE
                     ELHFPPLPELVIPEPERVVLDNGLVVLLMEDHELPLVGVSAMIRTGSRLEPEAKVGLA
                     SLTGTVLRTGGTKTLSGDALDEYLEGKAAAIETHIGDSSGTASMGSLTEDFPDVLKIF
                     GDVLRFPVFDPEKLKIAQNQVMAGISRQNDDPDEITSREFKKLIYGKESPYTWEPTYA
                     SVGSITREDVIRWHSTYFHPNRIILGVSGDFEREKTLALIQSIFGDWPKGPEVSDAQV
                     GIQANVPAGVFYVEKNDMTQAKIAMGHLGIRRDDPDYYPLVIVNQILSGSFGARLFYN
                     IRSKQGLAYDVHGGVGLQWDYPGMAILSMSTKTETTAKGIDALIAEARNMVAEPPTDE
                     EVTSAKASILNSFVFSVDSPTKVLGKYLTYEYYGYPSTWLREFRQGIEKVTTAEVRMA
                     AHKHLRPKEFAILVVGPRVGTKPALARYEKIQELDIRIPEPSAGM"
     gene            8090..8983
                     /locus_tag="PKM72_08335"
     CDS             8090..8983
                     /locus_tag="PKM72_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNP60831.1"
                     /translation="MVRTGLSCALLLSILLSGCQTNPYTNRSQLLLMPSGQMSQMGAA
                     QYSEVLQDPKVVISKDPKEIEPVKRVAARIIEAAKRSKYAEVAKGFDWEVTVIKDDNM
                     KNAWALPGGKIAVYTGIFPMAKNEAGLAAIMGHEVVHALAEHGGERMSQGLVAQYGMT
                     TASILLSTQGQNPALNALAMQAMGLGVQTGVLLPFSRKHESEADYIGILLAGDAGYDP
                     REAIHIWERMAATSDGAPPEFLSTHPAHETRIADLTKWMPEAMGLYEKAAKAPVGNLP
                     PIMPSPAQPPPQQPNGARLRH"
     gene            9001..10095
                     /locus_tag="PKM72_08340"
     CDS             9001..10095
                     /locus_tag="PKM72_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60832.1"
                     /translation="MVRPLPEKPGKSNPRYGMIVRPGLFWPTVVACMMLAGCGKSEAV
                     VEIALHPTNSKILYLATNDYIYKSRDEGMTWQNMSAGMTHSRVIALAIDPLFPANIVA
                     GTKGDAVYKSYDGGQRWVSRRTGLDDVTISSVVHQLVFAPGSSAHVYAATSLGVFESD
                     DGGETWSKRMEGMKEVLMVVALDCDPRQPQTLYAGTSGGVYKSIDGARSWRMVNNGLV
                     ASDVLKSSRALGVTRIKVDPRESDTVYTATLNGLYVTKDGGQVWTRIGDILPDQMLSD
                     LILDHTHPHVVYVTNRDGVFRSEDGGITWEARSEGLTNVNIRALAMSPVDSDVLYVGT
                     NGKGLFRTVDRGLSWKAVPLVVEESLGGLS"
     gene            10208..11488
                     /gene="clpX"
                     /locus_tag="PKM72_08345"
     CDS             10208..11488
                     /gene="clpX"
                     /locus_tag="PKM72_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNP60833.1"
                     /translation="MAFWDSKQGRNRADAVCSFCGRAKPGATNLIQAPTKLACTQCQA
                     PGSVLICADCIQQCSKFLSGHNEGPSVIEPPAGPLPTPPEIKAVLDQYVIGQDRAKKV
                     LAVAVHNHYKRVFSGKDLPDVELEKGNVMLIGPTGTGKTLLAKTLAKILHVPFAIADA
                     TTVTEAGYVGEDVENVVLKLLQSCDFNMARAQVGIIYIDEIDKISRKSESASITRDVS
                     GEGVQQALLKLVEGTLCNVPPKGGRKHPEQEFLRVDTSNILFICGGAFVGVEDIIAQR
                     TNQKRMGFGAVSANGEGLTSDQLMGKLKPEDLLKYGLIPEFVGRFPILASLNDLRVED
                     MVRVLQEPKNALLKQYQALFQLDGVQLDFTPEAVQAIATRASSMKTGARGLRSILEEV
                     MLDLMYDVPALKDIKRVVITEEAIDGKGVPQLIS"
BASE COUNT         2737 a         2593 c         3318 g         3012 t
ORIGIN      
        1 tggttgttct ggacgaagcc gatcggatgc ttgatatggg gtttgcccct cagctcaatc
       61 aaatcattga ggcgttaccg gaatcccggc aaaccttact cttctcggct acgatgccaa
      121 ataacctggc ggatttggcg cggatgtctc tcaacgatcc gtttaaagcc ttggtggcga
      181 aatcggccac accggctgaa ggcgtgactc ataccgtgca tcacacggcc aattccgaca
      241 aaacatccct cctgctttct attttaaagg agacagaagg ttcggtcctc gtgtttacca
      301 ggaccaaaca tcgcgctgac cggatcgggg aaaccctgga aaaggtcggt tgtcgcgttg
      361 cagtgttgca tgccggccga cggcttcctc aacgtcgagc ggcgttggaa gggtttcgcc
      421 gtggacgttt tgaaatctta gtcgcgactg atattgcggc ccgaggattg gacgtggacg
      481 atattcaaca cgtgatcaat tatgatttgc cccatgtgcc tgaagattat atccatcgta
      541 ttggtcggac ggcgcgtaag aataccaaag gttgggccac cagttttgtg accggtgagg
      601 ataatcgttc attacgaatg atcgaaaaat tgctggggaa ggcggttccc tgtgccccag
      661 gaagccggcc gccgatgccg cccgttgcac aggagagacg tgggcctcgg ccaaattccg
      721 gtggtagagg tttcggcgct ccccgatcgg gaagtccggc acggcatgac cgaccggctg
      781 gtcgccgacc gcgtccatct accttagctt cctaaaccat cacacgcagc ctctttatct
      841 tcataaaggg catcgcgggt tttttcttga agcccgggtc agaatgatcc gggcttcatc
      901 gtttttgaat tgtctctatt tctccccttt tctttttttc tcattttagt ccaggttgtg
      961 accccaggtt gtttatgggg tttttggctg attcgacaat gacctgcgtt acacttttct
     1021 gtatgagaat gtgcagggga ggtgcatcgg actgcgccgg tggcggttta aggccaaaag
     1081 ggaaacggcg aatcgcggaa gcaccgctgc taacgatcat acccctggta ttgccggttt
     1141 atgtcgctga gtaaagcctt tttgtcggtc ctctcgtgac catcgcaacc gacctgctct
     1201 tcggtgtggc atctgctcca cgcacaatcg aaatgacgaa gctcttcatg ccggataacc
     1261 tgccgtttga gaagggcctg tgtcctggat gattgaaaga tgaggacgaa gctatgcatt
     1321 ttgcactaga attcgctggt ggggaggtct ttttatttca ataaaaggag gtagtgacga
     1381 tgctatcctg gatccctcgg ctcactattg ctcttttgtg tatggggctt atggcgggtt
     1441 gtgcttccta ttcaacgggt acggtcaatc cacatgtggc ggaagcgtta acgcatgcac
     1501 aggaagctgt cgatcatggt ggtatggggc atgccgatgt ggttgtgacg catgcggaag
     1561 cttcccttca acatgcgcag gccgctaaaa aagacatgaa taatcctcag cttgatgcgg
     1621 ccattgccga gttaggggag gccattgccc atggtgaagc cgggcatgct gatgtggcta
     1681 ccagtcatgc caaatctgcg gtgatacatt taaaccaaat taatggaatg acaccctcaa
     1741 cagaaagcaa tgaaatggtg ccctcttcgg ggtattattg aaagggcttt ctgcaagatg
     1801 tttcgggtgg aagtgatgat gtgtcgggga accaagaacc ccgcctaaag tggcggggtt
     1861 cttggttgaa atattccctt aattttatgg ggcagtggca gttttgacgt ccaatatttt
     1921 cacgtcaaag tagagtgttt ttcccgctaa gggatgattg aagtctaaga cgaccacttg
     1981 ttctttcact tccgtcacgg tcggggtgac cattcgccct tggccatctt ttccttgtaa
     2041 ttttactccg acttttcgtg catcagtcgg aatttgatca atggggactt cctggacggc
     2101 ctgagggttg ataggcccat atccctcttc cggggcaacc gtcacctgct tactctcgcc
     2161 cgccttcatg ccatccaggg ctgtttccag tccaggaatg atttggtggg atccctgagt
     2221 aaacgtcaaa ggttctcccc ccacgttggt gtcaagcacc tctttgttct ccaacgtcaa
     2281 ggtatactcc attgagacgg actttccttc ggaaacggtc atgggtgagc ctccctcttg
     2341 tgaatccgcc tgtgcagatg tggccattgc cgaggccacc agcaagaagc tacagacaat
     2401 ccgagccaat acatgcagtg ccatgaaact gatcctccgc aaaaaatatt gctgatataa
     2461 cagttggctg gaaatggggg cggtttaaaa cgagatgggg aacaaccgtt tgcttccaga
     2521 ctgttggtga acgaatgcaa taatctatca gaaatatttt ttcttggcta tgtggtctgg
     2581 aaatattctg ctgcttgatg aaaagatgcc tgggattcat gagaacctca aaatttgtaa
     2641 actggtgaag actagagagg ccgatggttc agagcaggct ggccctcctt cttcgactat
     2701 tcctggcctg gcaacctcgc tatactaagc ggaacagtgt tttgctgtga gattttgatt
     2761 acgggaagga tgtttcgctt cctttagact gctctcctgg acccgaggag gaaaggccca
     2821 aaatggaacc caacgaacgg atggtaaaag cgaagcaaca ggttgcccaa catcggcggg
     2881 aattttcctg tcgggaggtg gatgaacctc aagatgagcg tgttccgccc gggcaacatg
     2941 tggtgaataa ttttccagtc cttgatttag gctgtaagcc ggagatcgtg ctggcggaat
     3001 gggccttaag tattgatgga tgcgtggcca atccattgac ctgggattgg gaggcctttc
     3061 aacgacagcc ccaagtgcag ttgactgctg attttcactg tgtgacgtcc tggagcatgt
     3121 tggataatga gtggggaggt gtgaaatttc aacatctgtt ggaattggtt cgtcccttgc
     3181 ccgaggcgaa gtatgtgtta ttcgaagcct ttgatgaata tacgaccaat gtgactctta
     3241 aggtttgcaa tgatgatgat gtgcttctgg caactcattg gagcgggagg ccattaacca
     3301 gggatcatgg tggcccggta cgcgtgatca ttcccaagct ttatggatgg aaaggcgcaa
     3361 agtgggtcaa aaaaattacg ttttccgaac gagatgagcg gggattctgg gaagtgcgcg
     3421 ggtattccaa taccgctctt ccctggaatg atgatcggta tggttgaaat gccgtgacgg
     3481 gaaaccgtgg tagagcgcgt ctcattttct cttctgtgga ctcttcatgt agttatgccc
     3541 gtgcgtgatc ctttctgtat taccaaaatt gaagcgtggc caatcgcctt gccgctcctg
     3601 gcaccctttg tcgtggcatc aggagccatg acggaggctc acaatgtctt tgtccgtgtc
     3661 acgcttcgaa acggggcgta cggttttggg gaaatggcac cctttcctga tatttcagga
     3721 gaggatcagg cgggttgcct tcaagtcttt cctcttgcag ccagggaatg tctgggacaa
     3781 tctgtcactc atttcagaaa attagcacag cagcttcgtg aggtggcccg ccggactcct
     3841 gccgtgcgat gtggccttga aacggcccta cttgatgcat tgtgccgtgg catgagtatt
     3901 cccttgtggg gactttgggg aggggcggat gtgcgacgac aggagaccga tgttacgttg
     3961 ccaattggac cactcgacca gatggtaggc accgcccaag cgtggtttgc caggggattc
     4021 cgggtattta aaatgaaagt cggcttggag gtggatgagg atatccggcg gatggaggca
     4081 gtgtctgccg catgtcctga ttcgaagctg gtccttgatg cgaatcaagg tttttcattg
     4141 gagcaggcaa gtgagtgtat aagagcaata gagcgattgc atctacctgt gatgttattc
     4201 gagcaaccgg ttgcccgaga ggatgttgag ggattcgtgg ccttgcgacg gaaaggaacg
     4261 attcctcttg ccgctgatga atcggttcga tccgtagagg atgcgagagg attgattgaa
     4321 cgaaacgccg tggatgtact gaatctgaag attacgaaat gcggggtggt ggagtctatg
     4381 gatattgccg ggttggcgcg ggcttcagga ctcgggctca tgattggtgg catggtagaa
     4441 agccgtgtgg cgatgggctg ctcctggagt ctggtgttgg gcttgggagg gtttgaattt
     4501 ttagatcttg atatgccgct actgttgtca ggggatccaa ttcaaggagg gtatcaatat
     4561 gagggacccg tattgcaccc ctgggaagaa tccgggcttg ggatggagat gagagatgag
     4621 ccctcatcgg tgatcgtcgt tgaataaaag gagcgttgag ctagaaggcc aagagtatta
     4681 ctcagtgtta ccatttctgg gttgtgcacg aatatctcta tcgcacgggc ggcggtccca
     4741 ttgtcaacag cttttctttt aattaggtaa ggcaaatctt ttttgccgca agtctcagga
     4801 caagattggt atcccgtcat aacacgagca ggtgttgtta agatgcggtt tccgcattca
     4861 atcgtttctt gcttgccaga attctcattt actgggtagg attaattaat ggtcagatta
     4921 gatacgttgc ttaaacagga tggtgattaa tgggaatcgg taaccgaatg atgggatgga
     4981 gcaagaccct cggacttgtg gggctggcgt ggttcgtcag cggagggttc gtgtttggtc
     5041 aggatctggc ctcctttgaa caacgtgtga cccagcatac gttaaagaac gggtggacct
     5101 tcataattgt ggaacggcca gtggcccctg tctttgcctt catgacgcgc gtcaatgtgg
     5161 gatcggctca ggaaatcatg gggcaaacgg gactggctca tatgtttgaa catatggcgt
     5221 ttaaaggaac accccacatt ggaacaaccg attacgacgc agaaaagatt gcgctagagg
     5281 cattggaaga ggcctaccag gcttatcaaa cggagaaatt ttcttccgat cctgacccgg
     5341 agagggtgga tcgcctgtat gcggttttta aaaaacgaca acaagaggcc tctgcctatg
     5401 tggtgaaaaa tgagttcggc gatatcattg aacgagaggg tggggtcgcc ttaaacgcgt
     5461 tcaccggtgc tgatgtgacc gggtattttt atgctcttcc ggccaataag gttgaactgt
     5521 ttgcctatct ggagtctgag cgctttctgc acccggtctt tcgagagttt tatgaggaac
     5581 gggacgtggt catggaagag cgtcgaatgc ggacggaaag ccaacccttc ggccggttgc
     5641 tcgaacagtt tgtcgcgacg gcctatatgg ctcacccgta tcatcatccg gtgattggct
     5701 ttgccagcga catccaatcc tacacgatga ccgacgcgaa gaagttttat gaggcgcagt
     5761 atgtgcctac aaatttagta acagcgatcg tcggggatgt gaaagcggag acggtcattc
     5821 cgattcttga aaaatatttc aatcgaattc cctctgctcc ctcgccccct ccgcttcgaa
     5881 tcgttgagcc tccgtccatc gccgaaaaaa ttgtgaaact gcaggatcct tcccaaccgt
     5941 tatatgtcga ggggtatcat aaaccccctg taactcatac tgatcaaccg gtgtacgatg
     6001 cgatcgacga tattttgacg aatgggagga cgtcccggtt gtatcgatcg ctgatacggg
     6061 acaaacgcat tgcggtttcg gtcggagcct atggggaata tccaggtgac aaatacccgc
     6121 atgtgtggat ggcctatgct gttcctggcc ggggcgtgac aaatcaggcc gtgcaacagg
     6181 ccttacgaga agagtttgac cggcttaaaa cccaggacgt ctccgatgag gagttggctc
     6241 gatttcgcac cagagcgaaa gccgggctag tcagggcctt gaataataac ctcggcatgg
     6301 cgatgtacct gactgattac cagatgttgt ttggcgattg gcgcgagtta ttccggtcca
     6361 tcgatcggtt ggatcaggtg accaagagcg aaattcgtcg ggtcgccaac acaatgtttc
     6421 aatccacgaa ccgaatcgtg ggcatgattg aaacggttcc tcctgccgat agtcctcaag
     6481 cagtcacttc cggggagggt caatcatgaa tcgcatcatg tctcagcagt ctgcggtgtg
     6541 tggagtaggc cgatggtcaa tcctgagcgc gggattggtc gtcttcgtca tgttttgtgt
     6601 acccgtttgg gtgcaagcgc aggtgactaa agtcgaggag ttgcattttc cacccttacc
     6661 cgaactggtc attcctgaac ctgaacgcgt ggttctggat aatggactgg tggtattgct
     6721 gatggaagac catgagttac cattggtggg tgtgtcagcc atgatcagga caggatctcg
     6781 gttggaacct gaagcaaaag ttgggttggc ctccttaacg ggaaccgtgc ttcgcaccgg
     6841 ggggacaaaa actctgagcg gagatgcgct ggatgagtat ttggagggga aggccgccgc
     6901 gattgaaaca cacattgggg actcgtcggg aaccgcatcg atgggttctc tcactgaaga
     6961 ttttccggat gtgttgaaga tattcgggga tgtgttgcgg tttcccgtat ttgatcctga
     7021 gaagttaaag attgctcaaa atcaggtgat ggcggggatt tcgcgtcaga acgatgatcc
     7081 cgatgagatt acgtcacgag aatttaaaaa gttaatttat ggaaaagaat caccatatac
     7141 atgggagccg acttacgcct ccgtgggatc tatcacgcgt gaggatgtga tccggtggca
     7201 ttcgacatac tttcatccta atcgcatcat tttaggtgtg agtggcgatt tcgaacgaga
     7261 aaagacgttg gcactcattc aatcgatttt tggtgattgg cccaaaggcc cagaggtatc
     7321 ggatgctcag gttggcattc aggcaaacgt tccggctggt gtattttatg ttgaaaaaaa
     7381 cgatatgacc caagccaaaa ttgctatggg acatctgggt attcgtcggg acgatcctga
     7441 ttattatcca ttggtgatcg tcaatcaaat tctttcgggg tcatttgggg cccgattatt
     7501 ttataacatc cggtccaagc aagggctggc ctatgatgtt catgggggcg tcgggttaca
     7561 atgggactat ccgggcatgg ccattctttc gatgtcgacg aaaacagaaa cgacggcgaa
     7621 gggaattgat gcgttaattg cggaagctcg gaatatggtc gctgagcctc ctacggatga
     7681 agaagtgacg agcgcgaaag ccagcatttt aaattccttt gtcttttcag tggattctcc
     7741 tacgaaagtg cttggtaaat acctgacgta cgaatattat gggtaccctt caacctggct
     7801 gagagaattt cgccagggca tagaaaaggt caccacggct gaagtccgaa tggctgcaca
     7861 caaacatttg agacctaagg agtttgccat tttggtggtg ggccctcgtg taggcacaaa
     7921 accggcattg gctcgttacg agaaaattca agaattggac attcgcattc cagagccttc
     7981 tgccggaatg tagatggtcc gcgctgcatg taacgggagc tggatgcctc tttgggatct
     8041 atgctgaatt cagagaggtt ctgagatgcc gatattgtgg agggccatca tggttcgtac
     8101 gggcttatcc tgtgcactgc tcctgagtat tctgctctct ggttgccaga ccaatcccta
     8161 tacgaatcgc tctcaactcc tcctgatgcc gtcggggcag atgagtcaga tgggggctgc
     8221 ccagtatagt gaagtgttgc aggacccgaa agtcgttatt tccaaagatc ccaaagaaat
     8281 tgagccggtc aaacgggtcg cggctcgtat tattgaggcg gccaagcgtt ctaaatacgc
     8341 ggaggtggcg aaaggatttg attgggaagt gactgtcatt aaagatgaca atatgaaaaa
     8401 tgcctgggct ttgcctgggg ggaaaattgc ggtctatacc ggcattttcc ccatggccaa
     8461 aaatgaggcc ggattagcgg ccattatggg gcatgaagtg gttcatgcat tggcggaaca
     8521 tggaggtgaa cggatgagcc agggactggt ggctcaatat ggaatgacca cagcgtcgat
     8581 tttactttcg acccaaggtc aaaatccggc gttaaacgcg ttagccatgc aggcgatggg
     8641 gcttggggtg cagacaggag tgctgttacc gtttagccga aaacacgaat cagaagccga
     8701 ttatatcggc atcctgcttg ccggcgacgc ggggtacgac ccacgggaag cgattcacat
     8761 ctgggaacgg atggcagcga cttctgatgg cgctccaccg gaatttttat ccacccatcc
     8821 agcccatgaa acccggattg ccgatctcac gaaatggatg cctgaagcca tgggtctgta
     8881 tgaaaaggct gcgaaagcgc ctgtgggcaa tctccccccc attatgccat ctcctgctca
     8941 acctcctcca caacaaccaa acggggctcg tctccgtcac tagctgtgga aggtgtccct
     9001 atggtgcggc ctcttcctga gaagccgggt aaaagtaatc caaggtacgg aatgatcgtc
     9061 aggccgggtt tattttggcc aacagtggtt gcctgcatga tgttggccgg ctgtgggaaa
     9121 agtgaggcgg tggtggaaat cgcccttcat cccacaaact ccaagattct ttacctcgct
     9181 accaatgact atatttataa atcccgggat gaaggaatga cctggcagaa catgtctgcg
     9241 ggtatgaccc attctcgcgt cattgccctg gccattgatc ccctgttccc ggccaatatt
     9301 gtagccggga ctaaaggtga cgcggtgtat aaaagttatg atggtggcca acgatgggtg
     9361 tcacggcgga cgggattgga tgatgtgacg atttcttctg tggttcatca attggtcttt
     9421 gctccgggtt ccagtgccca tgtttatgcc gccacctcgt taggggtgtt tgaatcagac
     9481 gatgggggag aaacctggag taagcggatg gagggcatga aagaagtgct catggtggtg
     9541 gcacttgact gcgacccccg gcagcctcaa acgctctatg ccggaacaag tgggggagtc
     9601 tataaatcca ttgatggtgc ccgaagctgg aggatggtca ataatgggct ggtggcctcg
     9661 gacgtcctga aatcttccag ggcactgggg gtgactcgca ttaaggttga ccctcgtgaa
     9721 tccgataccg tgtatactgc cactctgaat ggattatacg tcaccaagga tggggggcaa
     9781 gtttggactc gcattggaga tatactcccc gatcaaatgt tgagtgacct cattctcgat
     9841 catacccacc cccatgtcgt gtatgtgacc aatcgtgacg gtgtctttag aagtgaagat
     9901 ggaggaataa cttgggaagc tcgaagtgag ggcttgacga atgttaatat tcgagcttta
     9961 gcgatgagcc cggtcgattc cgatgtactc tatgtgggca cgaatggaaa aggacttttt
    10021 cggacggtgg accgtggatt gtcatggaag gctgtgccgc tggttgtgga ggaatccctg
    10081 ggaggtttat cctgaaaggg agatgatttg atatccttcc tcttgaagat taagatcatc
    10141 atttcatggg tgagtgatcg cactgattag attgatatct ggcgaagagt aaggaaaggt
    10201 aactgctgtg gctttttggg attcgaagca aggacgaaat cgtgcggatg cggtctgctc
    10261 cttttgtggt cgggcaaaac ctggagcaac gaatttgata caagccccca cgaaactggc
    10321 ttgtacgcaa tgtcaggcac cagggtcggt gttgatttgt gccgattgca tccaacaatg
    10381 ttccaagttt ttatcaggcc ataacgaagg tccctccgtc atagagccgc cagcgggtcc
    10441 cttgccgaca cctcctgaaa tcaaagcggt gcttgaccag tatgtgattg gacaggaccg
    10501 tgccaaaaag gttctggcgg ttgccgtcca caatcattat aaacgggtgt ttagcggcaa
    10561 agacctgcct gatgtggaat tggaaaaagg gaatgtaatg ttgatcgggc cgacgggaac
    10621 cggtaagacc ttactggcga aaaccttagc gaaaattttg catgtgccct ttgccatcgc
    10681 ggatgccact acggtgacgg aggcgggata tgttggagaa gacgtcgaaa atgtggtctt
    10741 gaagttattg cagtcgtgcg attttaatat ggccagagcc caagtgggta ttatctatat
    10801 cgatgaaatt gataagatta gccggaaatc tgaaagcgca tctattacgc gggatgtgtc
    10861 cggggaaggt gtccaacaag cattgctgaa attagtggaa gggacgttat gtaatgtgcc
    10921 tcccaagggg ggcagaaaac atcccgagca ggaattcctc agggtggata cctccaatat
    10981 tctctttatt tgtggcggtg cgtttgtcgg agtggaggat attattgctc aacggaccaa
    11041 tcagaagcga atgggttttg gggcagtttc cgctaatggt gagggactga cctctgatca
    11101 attaatggga aaactcaagc ctgaggatct tttaaaatat ggactcatcc ctgaatttgt
    11161 gggcaggttc ccgattctgg cgtctctgaa tgatttgcgc gtggaggata tggttcgggt
    11221 tctccaggaa ccgaaaaatg ctcttctgaa gcaatatcaa gcattatttc aattagatgg
    11281 ggtgcagttg gattttaccc ctgaggcggt tcaggccatt gccacaaggg cctcgtctat
    11341 gaaaaccgga gccagaggat tgcgttccat tctggaggaa gtgatgctgg atttgatgta
    11401 tgatgttcca gccctgaaag atattaagcg agtggtaatc actgaagagg cgattgatgg
    11461 caagggtgtt ccccaattaa tttcctgaaa ataatgctct tcaatagttg tttctcgcct
    11521 ggggtctgtt gcattctttc ccattctgac ttatgcttat caaaagaaat tcatcacgac
    11581 attcgggcca cgtggtgaat gtttgagtga cgggcccatc actgatgttg ttttgccaag
    11641 gaatccgcaa ggagtctgat
//
LOCUS       DAORAQ010000076        11607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_918_length_11607_cov_60.479744, whole genome shotgun sequence.
ACCESSION   DAORAQ010000076 DAORAQ010000000
VERSION     DAORAQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_918_length_11607_cov_60.479744"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1297)
                     /locus_tag="PKM72_08350"
     CDS             complement(26..1297)
                     /locus_tag="PKM72_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNP60834.1"
                     /translation="MVLGPFAEPKVARRTGAKPRTALKNVPSRAEPNKRPIQLTLLNV
                     IEPNICYNRLMTYNWQQPDWPDFQYDLTTVEDALLAFAEKTGRASGLLSGIPQETQTE
                     AIIEMMVVEALKTSEIEGEYLSRQDVLSSIRKNLGLGQPGEQVRDQRAQGAAALMTDV
                     RTRYAGPLTKATLCSWHRMIMQGSRGITVGKWRNHAEPMQVVSGPIGKPRIHFEAPPS
                     TRVSKEISRFITWFNDTGPQGKKDIKKPLIRSAIAHVYFESIHPFEDGNGRIGRALSE
                     KALSQGLGRPALLSLSRAIEANRAHYYEALQKAQQSNHITPWISWFVNIALAAQQQAE
                     EQIEFTLRKTRLFDRLQDQLNSRQMQVVRRMLEEGPSGFTGGINAKKYMAIAHTSKAT
                     ATRDLQDLVAQGALTLVGGGRSTRYEINLGQ"
     gene            complement(1509..1844)
                     /locus_tag="PKM72_08355"
     CDS             complement(1509..1844)
                     /locus_tag="PKM72_08355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNP60835.1"
                     /translation="MTCSAYDLVLIPFPYADLSSSKKRSVLVLTAPDHHGDFIALAVT
                     TVPQEEPAIPLTAIDLVDGTLPKPSWIRVDKVFTLSDQNIVKHIGKIKTACMEKVLKS
                     LCSIVGYAK"
     gene            complement(1834..2034)
                     /locus_tag="PKM72_08360"
     CDS             complement(1834..2034)
                     /locus_tag="PKM72_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60836.1"
                     /translation="MSTAEQIMREIQKLPEPLAKEVLDFIGYIELKHGLKDRLAEELK
                     LAQTPAMKHAWDNSEDEVWNDV"
     gene            complement(2531..3718)
                     /locus_tag="PKM72_08365"
     CDS             complement(2531..3718)
                     /locus_tag="PKM72_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNP60837.1"
                     /translation="MRLHHIPRTIHPRQEIPSDQPYRLSTEWQRLLLPVLFVTLLLPH
                     TAMGEALVPKQAQSVPARPSHLYLVAQQSLGPEELNTIAVFEKAAQSVVYITNTAVRR
                     DIWSLNTFEVPQGSGSGFIWNRQGHIVTNYHVIYGADSIQVVLDDQSTRDARIIGVDP
                     DHDLAVLQITGKTDTLMPLDIGNSQDLRVGQRVLAIGNPFGLDHTLTTGVVSALGRSI
                     KSVNDRTIEGAIQTDAAINPGNSGGPLLNSAGQLIGVNTQIVSPSGAYAGIGFAVPVD
                     IVKRVVPQLIQHGKVIRPGLGISLIPDAIAARWGIKGIVIAKVLPGSMADKAGLQGLE
                     ETANGRIRLGDVITAIDGETLRTYDDLARMLDRHNVGDRIKLGIRRNGKEHTLSLQLQ
                     AVQ"
     gene            complement(3743..4165)
                     /locus_tag="PKM72_08370"
     CDS             complement(3743..4165)
                     /locus_tag="PKM72_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNP60838.1"
                     /translation="MSVITHSLNISMTGDSNIENLTGHIQRILAESGLQGGILTLFVQ
                     HTTASVMIIEDEPGIRADTKKFWNRTIPADPHLEHNTRNAGEDNGHSHLRGQLQGPSL
                     TIPFVNGALTLGTWQQVVLIDFDTRPRTRNLVLQLMGE"
     gene            complement(4174..5139)
                     /locus_tag="PKM72_08375"
     CDS             complement(4174..5139)
                     /locus_tag="PKM72_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60839.1"
                     /translation="MTAPQGWDILPHSYTLHNPYAFCHEDYIILAVVSRQTLKSTHTV
                     LTRTQVVSLLKWYDRPHPTRPDRMIPGYDKAHALRTSRMCVAVAAALGHPLSRLRQFE
                     AACLLHDMGRAGLDPVLFGQIWSWAKEQKIPTRPREWRTRYPDTPYGRESHAFIAQYG
                     AALQARGVKLTPAVKDHIDMRLGFARRLKKYLRPVKTAIATIDIPWSPWMERIMLYYY
                     YPEKLERAAAWTHQLAEILVACEQLEAYSNHRRGKDYYARAEESFAAAFTYLRQLTFQ
                     RIVSQEVLDLICRLTREGRFTSVLRQARGGTLSPADQQFLQTVSC"
     gene            5394..8753
                     /locus_tag="PKM72_08380"
     CDS             5394..8753
                     /locus_tag="PKM72_08380"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNP60840.1"
                     /translation="MRWSGKQMFWWGAFAALILTIALIVLPLFLNPDYLKNLALQQIQ
                     RTFGSHVTVGQTSFALFPSPHFLVSDIVVQEQPESHAVFRAKSMSLDLGIWPLLMKKI
                     VVREFLVESPEIELRRESGGEWRFLSYASNDSPIVSLAEFLVLGKFEVTDGKIIVIDE
                     SPRESVRGFVIEDVAFVSETSHEGSSIDSTFDLSGKIRQTEELAPFRIKGELEALWET
                     PLHSVINEPVGFEQITFSGKVRADDLEIKQIGEFLPNGQSLVEVPGTLSVKSQVTWVQ
                     NAKTSRLSLSKINLSSSFIDLDGSANTEMLEDGHHMMGFSMRSSNVDLDMIRQYLPRT
                     WMPDDLLPVWGTGEWGGGLNIAEARVTGSTREDVRTSVTGTFRLTDGYLRIPDWPTTD
                     HIQGTVVVEPDRIQLSEVHGIYDGIPVEVTEGLFLLKESGPWGNVGIQGPVPAEKVRQ
                     VVTQLGAPTDFGLLSSWKVLDGTGLLRLQFTGNLLESPGLMFQSGEFNPEGLVIQIPG
                     LPHPLTEGHGTIIFSRESTVFQGIQGNVGAFPLALNGTINYQGVPRFDPLTIQGGFAG
                     EDLLPVSETQATSPGMQMTGPLTASVILTGPTGHPKLKGWIDGRDALIAVPSLLRKHA
                     GQDGRLEFEGQFRPGSRVHFERVELIMLPLRLRGQGTIRYRPKVTWEARFDSGPVYLG
                     VLPEGIRVLGDIIQSGILEVQLKGSGRGTDWTQWNTKGWVALTEGVVALRGMTDPISN
                     LFVRLKVDENLLDLKRMEFRLKNSEAVITGFMKNWKTTPIVSVLFDSPQFDIDLLIPK
                     EGRSAIRDGIEWLAAHGSLEGSVYIDRPSYKTLSGKKLSGALRIHDNLVSVDKVQAMV
                     AEHGTVGGRFFVHLPENRPAAMRASFQAKDLPFENVLSLFGEERRWITGKMAVRGMLQ
                     GHGRDDRGIIPTLNGSLEVSLGEGYIRKGTIVPRILTLLNLPHVLRGKFDLEANGFPF
                     TTVSSKLTVEDGVFSTKDMLIQSPIMKISAAGKYDWKGDRLEGISAVSPFGAYSDLLK
                     DLPLFGRIFSGDRKGIATAMFSIAGPLGEPEVEYLPMESVKTGLTGLAKLAFDVLKNT
                     LTLPYDLLKGSKGDEDSLSPDSNISGVPGPL"
     gene            8861..>9488
                     /locus_tag="PKM72_08385"
     CDS             8861..>9488
                     /locus_tag="PKM72_08385"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="s-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNP60841.1"
                     /translation="MIPTVEWKNNHVVILDQRALPGEVRFLHCGTYQEVAEAIRNLSV
                     RGAPAIGVTAALGIALGARQCEETQHERFSGYMEEVCRTLAATRPTAVNLFWAVDRMK
                     RVMTDVSSAPVQEIQKRLQEEALVILEEDLRVNRAIGTFGAAIIRDGDHILTHCNAGA
                     LATAGYGTALGVVRAAWEQGKKIRVFADETRPVLQGARLTAWELQQDGI"
     assembly_gap    9489..9588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9589..10056
                     /locus_tag="PKM72_08390"
     CDS             <9589..10056
                     /locus_tag="PKM72_08390"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNP60842.1"
                     /translation="PVLQGARLTAWELQQDGIPVTLITDNAAGALMRQGRIHCCVVGA
                     DRIAANGDVANKIGTYSVAVLAHAHGIPFYVAAPSSTIDMETADGNGIPIEERSPSEV
                     TSLHSGQMIAPHGVEVWNPAFDVTPADLIAGIITERGIFAPQALSDYFSSSRS"
     gene            complement(10069..>11607)
                     /locus_tag="PKM72_08395"
     CDS             complement(10069..>11607)
                     /locus_tag="PKM72_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60843.1"
                     /translation="GELQRKKAEDALRDSEQRYRAIVEHAYDLIIETTATGECQYVTP
                     NCLKVLGYGAADMLGKSFFDFVHPEERGPLSASFHSHLKALQEIDMVCRLQTKQSEWR
                     WFESHIHPFRTSVGTVVGVIVTRDITEWKRLEEERIRATKLESVGLLAGGIAHDFNNI
                     LTSVFANIGLAKMVTAKHTPPHGEKVIERLEAAEKACLRARDLTKQLLTFAKGGAPVK
                     STTSVASIITDTIQFALRGSAVRCTTHLPDDLWPVEVDEGQISQVIQNLVINSDQAMP
                     EGGTISVVAKKFLVNAQSTLPLKSGKYVCVSVADQGIGIPDDHLQKIFDPYFTTKQKG
                     SGLGLATTYSIMKRHGGHVTVTSSLGHGTCFTLYLPASTSLSTKNEETPESLVQGTGR
                     ILVMDDEEDIQDVLGKMLEHLGFDVDYTDDGLKAIALYTRAVQDGNPYVATILDLTIP
                     GGMGGKETLRLIKDYDPDAKVIVSSGYSNDPVMAHASQFGFSGFIAKPYNLLDLSKVL
                     SRIF"
BASE COUNT         2477 a         2767 c         3369 g         2894 t
ORIGIN      
        1 gtttcccttt ttttattctg cttccttatt ggccgagatt gatctcatac cgtgtgctgc
       61 ggccgccacc taccagggtg agtgcgcctt gtgccaccag gtcctgtaaa tcccgtgtgg
      121 ctgtcgcttt ggaggtgtgg gcgatggcca tatatttttt ggcattgatc ccgcctgtga
      181 atccgcttgg tccttcttct aacatccggc gcaccacctg catctgtcgt gaattgagtt
      241 ggtcttgtag tcgatcgaac aatctcgttt ttcgcaaggt gaattcaatc tgctcttctg
      301 cctgctgttg cgcagccagt gcgatattca caaaccagct tatccagggt gtgatgtggt
      361 tggattgttg ggctttctgc aaggcttcat aatagtgtgc gcggtttgcc tcaatggcac
      421 gagagaggct gagcagagcc ggccgcccga gaccttgcga taaggctttt tccgacaggg
      481 cgcgcccgat gcggccgttt ccgtcctcaa acgggtggat ggattcaaaa tagacatggg
      541 cgatggcgga gcgtatcagg ggctttttga tgtccttctt gccctgtggg ccggtgtcat
      601 taaaccaggt gatgaaccgc gaaatttctt tggaaacccg tgttgagggc ggggcttcaa
      661 aatgaatgcg gggttttccg atcgggccgg ataccacctg catgggctcc gcatggtttc
      721 gccatttccc gacggtgatg ccacgactgc cttgcatgat catgcggtgc caggaacaga
      781 gtgtggcttt ggtcagaggc ccggcatacc tggtgcgaac atccgtcatg agcgcggcgg
      841 ccccttgcgc ccgttgatcc cgcacctgtt cgccaggctg tcccaatccc aggtttttcc
      901 ggatggagga gaggacatcc tgacgactta aatattcgcc ttcaatttct gatgtcttga
      961 gagcttccac gaccatcatc tcgatgatgg cttccgtctg cgtctcttgc ggtattccgc
     1021 tcaacaggcc gctggctctc cctgtttttt cggcgaatgc cagtaaagcg tcttcaacgg
     1081 tggtgaggtc gtattggaag tccggccagt ccggttgttg ccagttatag gtcatgagcc
     1141 gattataaca gatattcggc tcaataacgt tcagtaaagt gagctgaata ggccttttat
     1201 tcggctcagc ccgtgaaggg acatttttta gtgcagtccg gggtttcgcc cccgttcgac
     1261 gagccacttt tggttcggca aaaggaccca aaaccattga cgcccagtct ggttttattg
     1321 aaggggaggg acgctaactt tttgaagagc ggcccaactc gcgggggctc aaacaagggc
     1381 cgctagctgc caagagcgtc cctcactggg gccagacggc aggtgtcggt tgaagagaaa
     1441 aggagggttt gcataaaagg aaagattacg ccgaaggatg gagtgagaag atgtttgcgg
     1501 gtagggcgtc atttcgcata accgacaata ctgcatagac tttttaggac cttttccatg
     1561 catgccgttt tgatctttcc gatgtgcttg acgatgtttt ggtctgagag ggtaaatacc
     1621 ttatccactc gtatccaact gggtttgggc agagtaccat ccacgagatc tattgcagtt
     1681 aatgggatgg caggttcttc ttgagggaca gtggtaacgg ccaaggcaat aaaatcccca
     1741 tggtgatcgg gtgctgtcag gacgaggacc gaacgctttt ttgaagaact taaatcggca
     1801 taaggaaagg gaatcagaac aaggtcatag gcgctacacg tcattccaaa cctcatcctc
     1861 agaattgtcc caggcatgtt tcatggccgg agtctgggcg agcttgagtt cctcagctag
     1921 cctgtctttg agcccatgtt tcaactcaat gtacccgatg aaatctaaga cttccttcgc
     1981 taaaggttct ggaagctttt gaatttctct catgatttgt tcggccgttg acatggcgtg
     2041 ctcctcattt cctctgtcag ggatacgttg tacgaccaat gtacccaacc cattgattaa
     2101 agtacagaat tttgtagagc cttttttaga gccggggggg ggcggccaga cagccgagcc
     2161 aattttgttt tggccaaagt ggccacagct attgacgttc cctctggcct cattggagga
     2221 gagggacgct aatcggagaa gagcggccaa actcgcgggg ctaaaacaag ggccgctagc
     2281 tgccaagagc gtccctccct ggagccggcc ggcaggcgtc ggatcaaagt taaaacgtgg
     2341 cggtggagat ccatggaggg caggccaact cgcagggctt aaaattttaa aaatagaaga
     2401 atagaaattt tggtgattcc ttgtttaagt accaagatct ttcgctgcgc ttaggatgac
     2461 attttttgga ggaagttctg aaacggtttg caggaatgac ggatgattct tagcggcaag
     2521 gaacggttat ttattggacg gcttggagct ggagggagag cgtgtgttct ttgccgttgc
     2581 gtcggatgcc gagtttgatg cggtctccga cattgtgccg gtctaacatg cgagccaggt
     2641 cgtcataggt tcgtagcgtc tctccatcaa tggcggtgat cacgtcacct aatcgaatcc
     2701 gtccgtttgc cgtttcttct aagccttgca ggcccgcctt gtccgccata cttcctggaa
     2761 gaactttggc aatcacgatg cctttgatcc cccagcgggc ggcgatggca tccgggatga
     2821 gtgagatgcc caggccggga cgaatgactt ttccatgttg aatgagttgc gggacgacac
     2881 gtttgacaat gtcgacgggc accgcaaagc caatgcccgc gtaggctcca ctgggactga
     2941 caatctgggt gttgacgcca atgagttgcc cggcgctgtt taagagcggt ccaccggaat
     3001 tgccggggtt gatggccgca tcggtttgga tggcaccttc aatcgtccgg tcattcaccg
     3061 atttaatcga acgtcccagg gcactgacga caccggtggt gagggtgtga tctagtccga
     3121 agggattgcc gatggccaat acgcgttgac cgacccgaag gtcctgggaa tttccgatat
     3181 ccaagggcat gagtgtgtcg gtcttgccgg tgatctggag cacggccaga tcatgatcag
     3241 ggtcgacccc gatgatgcgc gcgtcacggg tggattgatc gtccagcacc acttgtatgg
     3301 aatccgcgcc atagatgacg tggtaattgg tgacaatatg tccctgccgg ttccagatga
     3361 atccggatcc cgacccctgc gggacttcga aggtattgag cgaccagata tcccgacgga
     3421 cagccgtatt ggtgatgtag accaccgact gtgcggcttt ttcaaagacg gcaatggtat
     3481 tgagttcttc tggaccaagc gattgctggg cgacgaggta tagatgcgag ggtcgggcag
     3541 gaaccgattg cgcctgtttc gggactaatg cctcgcccat ggcggtgtgt ggcaaaagaa
     3601 gcgtgacgaa caggaccggg aggagaagcc tctgccattc ggtggagagc cggtatggtt
     3661 ggtcgctggg tatttcctgg cgaggatgga tggtccgggg aatgtggtga agacgcatac
     3721 gaaagtgttt cccggttcgg ttctattctc ccatgagctg gaggaccaga tttctggttc
     3781 gtggtctggt atcaaaatcg atcaaaacga cttgttgcca cgtgcccagg gttaaggcac
     3841 cattcacgaa cggaatggtc agcgaaggcc cctgtaattg accgcgaagg tgggagtggc
     3901 cgttatcttc tccggcgttc cgcgtattat gttctaaatg aggatcagcc ggtatggtgc
     3961 ggttccaaaa ttttttcgta tcggcacgaa tgcctggctc atcttcgatg atcatgaccg
     4021 aggccgtcgt atgttgcacg aagagggtga gtattccacc ttgaaggccg gactctgcga
     4081 gtatgcgttg aatgtgccct gtgaggttct cgatgttcga atcgccggtc atggaaatgt
     4141 tgagggagtg agtgatgacg gacatgggca tccttagcac gagacggttt gtaaaaattg
     4201 ttgatcggct ggtgaaaggg tgcccccgcg cgcctgtcgt aaaactgacg tgaaacgccc
     4261 ttctctggtg agccggcaga tgagatccaa gacctcttga ctgacgatcc gctggaaggt
     4321 gagttgacgg agataggtga aggcggcggc aaaactttct tctgcgcgcg cgtaataatc
     4381 ttttcctcgc cggtgattgc tataggcttc caattgttcg caggcgacta agatttctgc
     4441 gagttggtgt gtccaggcgg cggcgcgttc gagtttttcc gggtaatagt agtacaacat
     4501 gatgcgttcc atccagggcg accaggggat gtcgattgtg gcgatggccg ttttcaccgg
     4561 acggagatat tttttgaggc gtcgggcaaa tcccaaccgc atgtcgatgt gatctttgac
     4621 cgcaggggtg agcttcacgc ctcgggcttg gagtgcggct ccatactggg cgatgaaggc
     4681 gtgcgattca cggccatagg gggtgtcggg atagcgggtg cgccattccc gcggccgggt
     4741 cgggatcttc tgttccttgg cccatgacca gatttggccg aacaggaccg gatcaagccc
     4801 ggctcgcccc atatcatgca gcagacaggc ggcttcaaat tgacggagtc gggagagtgg
     4861 atgtcctaga gccgccgcga ccgccacaca catgcgtgac gtccggagcg catgcgcttt
     4921 gtcatatccg ggaatcatgc gatcgggtcg ggtgggatgc ggccggtcgt accatttcag
     4981 cagcgagacc acctgtgtgc gggtgagaac ggtgtgggta gatttcaatg tttgccggga
     5041 aacaacggct agaatgatat aatcctcatg acaaaaagcg taaggattat gcagtgtata
     5101 agagtgtggc aggatatccc agccctgggg agctgtcaag aaaccgctgg ggcacccaac
     5161 atgggacggc tatccgataa cgaaccacga cgatgccaat ttccccggat ggcgggccgg
     5221 gtagggcgaa tgacttctgc cgtctgtgct tccctgcagt ccatggaggg ttggagtcat
     5281 tcttacattt ccaacggatt tcggtatgat ggagaagtaa ttttcatggt tcccattcct
     5341 cacgtcacac acacaacggt tagggtatct gctcatccga ggagagtcat gtagtgcgat
     5401 ggtccggcaa acagatgttc tggtggggcg cattcgctgc cctcatcttg acgattgcgc
     5461 tgatcgtgct tcccttgttt ttaaatcccg attatctgaa gaatctggct ctgcaacaaa
     5521 ttcaacgcac gtttggttcg cacgtgacgg tgggacagac ttccttcgct ctatttccct
     5581 ctccgcattt tttggtttcg gacattgtgg ttcaggagca gcctgagtcg catgcggtgt
     5641 ttcgtgcgaa atccatgagt ttggacttgg gaatctggcc gttgctcatg aaaaaaattg
     5701 tggtgcggga gtttttggta gagtctcccg aaattgaatt gcgtcgtgag tcaggggggg
     5761 aatggcgttt tctgagctat gccagcaatg attcgcccat tgtgtcctta gcggaatttc
     5821 tggtgctggg aaaatttgag gtgacggacg gcaaaattat cgtgattgat gagtcacctc
     5881 gagaaagcgt tcgaggattt gttattgaag atgtggcctt cgtctctgag accagtcatg
     5941 aagggtcctc aattgactcg acgttcgatt tgtctggaaa aatccgtcag accgaagagt
     6001 tggcgccttt tcgcatcaag ggggaacttg aagcgctttg ggagactccg ctgcattccg
     6061 tcatcaacga gccggttggt tttgagcaga ttaccttctc cgggaaggtg agggctgatg
     6121 acttggagat caagcaaatt ggggaattcc tgccgaacgg tcaatcgctg gtggaggtcc
     6181 ccggtacgct gtcggtcaag tctcaggtca cctgggtcca gaatgcgaaa acttcccgac
     6241 tttctctctc taaaattaac ctgtccagtt cttttattga tttggatggt tcggccaata
     6301 ctgaaatgct ggaagacgga catcatatga tggggttctc catgcgatcc tccaatgtgg
     6361 atctcgacat gattcgacaa tatctgccca ggacgtggat gccggatgac ctgctgcccg
     6421 tatggggcac aggcgagtgg ggaggcggac tgaatattgc cgaagcacgg gtcaccggct
     6481 ccacgcgtga agatgtgagg acttccgtca ccggcacgtt tcgtttaacc gatggctatc
     6541 tccgtattcc tgattggcct accaccgacc acatccaagg gacggtggtg gtggaacccg
     6601 atcgaattca attgtccgag gtgcacggca tttatgacgg tattccggtt gaagtgacgg
     6661 aaggcctctt tttgttaaaa gagtcaggcc cgtgggggaa tgtgggcatt caagggcctg
     6721 tacctgcgga gaaggttcgg caggtcgtga cgcagttggg agcgccaacc gattttggtc
     6781 tgttgagttc atggaaggtc ttggatggca ccggcctgct tcgcctgcag tttaccggga
     6841 atcttttgga atctcccgga ttgatgttcc agtctggtga gtttaatcct gagggcctgg
     6901 tgattcagat tccgggtctt cctcatcctt tgactgaagg ccacggtacg attatttttt
     6961 cccgggagag taccgtgttt caaggcatac agggaaatgt gggtgccttc ccgctggcgt
     7021 tgaatgggac tatcaactat cagggagtcc cccggtttga tccgctcacc attcagggcg
     7081 ggtttgccgg tgaggacctg ttgccggttt ccgagactca ggcgacatca cctgggatgc
     7141 aaatgaccgg tccgcttacc gcctcggtca ttctgaccgg tccgaccggt catcccaaat
     7201 tgaaagggtg gattgatggg cgagacgctc tcattgccgt accgtctctt ttgcggaaac
     7261 atgcgggtca ggatggacgc cttgagtttg agggacagtt ccgtcccggg agtcgggtgc
     7321 attttgaacg agtggaactg atcatgctgc ctcttcgtct tcggggacag gggaccatac
     7381 ggtatcgccc gaaggtcaca tgggaggccc ggtttgattc cggaccggtg tatttaggtg
     7441 tactgccgga aggcatccgt gtcctgggag atatcatcca gtcaggaatt ctggaagttc
     7501 aactgaaagg cagcggacgc ggaaccgatt ggacccagtg gaacaccaaa ggttgggtcg
     7561 ccttaaccga aggggtcgtg gctcttcgcg ggatgaccga tccaatttcg aatttgtttg
     7621 ttcgactgaa agtggatgag aatctgcttg atctgaagcg catggagttt cgcctgaaaa
     7681 atagtgaagc cgtcatcacg ggatttatga aaaactggaa aaccacaccg atcgtcagtg
     7741 tcctgtttga ctcgcctcaa ttcgatattg atttattgat tcccaaggaa ggacgatcag
     7801 ccatccggga tgggatcgag tggttagcag cgcatggatc gctggaaggt tctgtgtata
     7861 tcgaccgtcc gagctataag acgctttccg gaaaaaaatt atcgggcgcg ctgaggattc
     7921 atgacaacct ggtgtcagtg gataaagtgc aagcgatggt ggcggaacat ggcaccgtcg
     7981 gggggcggtt ctttgttcat cttcctgaaa atcgaccggc cgccatgcgc gcgtcatttc
     8041 aggcgaagga tttgcctttt gagaacgtct tgagtctctt tggcgaggaa cgtcgatgga
     8101 tcaccggaaa gatggctgtg cgaggcatgc tccaaggcca tgggcgggat gaccgcggca
     8161 tcattcccac tctcaatgga agtcttgaag tttcgctggg cgaaggctat atccgtaaag
     8221 gcactattgt cccgcgaata ttgacgcttt taaatttacc gcatgtcctc aggggaaaat
     8281 ttgatcttga agcgaatggg tttccgttta cgaccgtgag cagcaaactt acggtggaag
     8341 acggtgtatt ttccaccaaa gatatgttga tacagagtcc gatcatgaaa atctctgcgg
     8401 caggcaagta tgactggaaa ggtgatcggc ttgaaggcat ttccgccgtc agcccttttg
     8461 gggcctattc ggatctcttg aaagaccttc ctctctttgg acgaattttt tccggagatc
     8521 ggaagggcat cgccacagcc atgttttcaa ttgcaggacc gttgggtgag ccggaggtcg
     8581 aatatcttcc catggaatcc gtgaaaacag ggttgaccgg tttggccaaa ttagcgtttg
     8641 atgttttgaa aaatacgttg actctgccct atgatttatt gaaaggctct aagggagacg
     8701 aggattccct ctctcctgat tccaacatat ccggcgtacc cggtcccttg tgagccgcgt
     8761 atctttgccg atgcgacttt ctgatggacc ttgagagaaa gtgggaaggg tgatgaacca
     8821 attgaggtgg cgagaacaga tcgagtgaaa ggaagccagg atgattccaa cggttgaatg
     8881 gaaaaataat catgtcgtga ttctggatca acgggccctc cccggagaag tgcgatttct
     8941 tcattgtggc acctatcagg aggtggctga agcgattcgg aatttgagcg tacggggtgc
     9001 gcctgccatt ggagtgacgg cggctttggg catcgcctta ggggcccggc aatgtgagga
     9061 gactcagcat gagcggtttt ccggctatat ggaagaggtc tgtcgcacgc tggccgcgac
     9121 gcgtccgacc gccgtgaatt tattctgggc cgtcgaccgg atgaaacgtg tgatgacgga
     9181 tgtgtccagt gcacctgttc aagaaatcca aaaacgatta caggaagaag ctctggttat
     9241 tttggaggag gaccttcggg tgaaccgggc cattggcacg tttggcgccg caattattcg
     9301 tgatggagat catattctca cgcattgtaa cgccggagcc ctggcaacgg caggctatgg
     9361 aacggccttg ggagtggtca gggcggcctg ggagcagggg aaaaagattc gggtctttgc
     9421 cgatgagacc cgacccgttc tccaaggggc ccgtctgacg gcctgggagc ttcagcagga
     9481 tggtattcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cgttctccaa
     9601 ggggcccgtc tgacggcctg ggagcttcag caggatggta ttcccgtgac gttgattacc
     9661 gataatgcgg cgggggcact gatgcgacag ggccggattc actgttgcgt cgtgggggct
     9721 gaccggattg ctgccaatgg cgatgtggcc aataagatcg gaacctattc ggtggcggtc
     9781 ctggcccatg cccatggcat ccctttttat gtggccgcac cttcatcgac cattgatatg
     9841 gaaacagcgg atgggaacgg cattcccatt gaagagcgga gcccgtccga agtgacctct
     9901 ttgcatagtg ggcaaatgat tgctcctcac ggtgtggaag tctggaaccc ggcgtttgac
     9961 gtgaccccgg ccgacttgat tgccggtatc atcactgaac ggggtatttt tgctccacaa
    10021 gccttatccg attatttttc gtcgagccgt tcctgattga gggtaccgtc aaaagatacg
    10081 acttaagact ttagacaaat cgagcagatt atacggtttg gcaataaaac ctgaaaatcc
    10141 gaattggctg gcatgcgcca tgaccgggtc gttggaatac ccgcttgaaa caatcacctt
    10201 ggcatcggga tcatagtctt taatgaggcg caacgtttcc ttccctccca ttcctccggg
    10261 tatggtgaga tcaagaatgg tggccacata gggattgccg tcctgaacgg cacgagtata
    10321 gagcgcaatg gctttcagcc catcgtcggt atagtccacg tcaaatccga ggtgctcgag
    10381 cattttccct aacacatcct ggatgtcctc ttcatcatcc atcactaaaa tccgtccggt
    10441 tccttgcacg aggctttccg gtgtctcctc gtttttcgtc gacagggagg ttgaagcggg
    10501 gagatacagg gtgaagcagg ttccgtggcc gagagaagag gtcacggtca catgccctcc
    10561 atgtcgtttc atgatggaat aggtcgtggc gaggcccagc ccgctccctt tttgtttggt
    10621 tgtaaagtag ggatcaaaga ttttttggag atggtcgtca ggaatgccga tcccttgatc
    10681 agcgacggac acacagacat acttgccgga tttgagaggg agggtggatt gagcgttcac
    10741 cagaaatttc tttgcgacga cgctaatggt gcctccctcc ggcatcgcct ggtcgctatt
    10801 aatcacaaga ttttgtatga cctgactgat ttgtccttca tcgacctcaa ccggccagag
    10861 gtcatcggga agatgcgtgg tgcatcggac ggcagagcct cgtagggcaa attgaatcgt
    10921 gtcggtaata attgaggcca ccgatgtcgt gcttttaacc ggagcccctc ctttcgcaaa
    10981 ggtcagcagt tgcttggtaa ggtcccgcgc acgcaaacag gctttttctg ccgcctccag
    11041 tcgttcaata accttctctc cgtggggtgg cgtatgtttg gccgtgacca ttttggccag
    11101 cccgatattg gcaaacacgg acgtgagaat attattgaaa tcatgcgcaa tcccgccggc
    11161 cagtaaccca acagactcga gtttggtggc acgtatgcgt tcttcctcca ggcgtttcca
    11221 ttccgtgatg tctcgtgtca cgatcacgcc cacgaccgtc ccgacgctgg ttcggaaggg
    11281 atggatgtga ctttcaaacc atcgccactc agactgtttc gtttgcaacc ggcacaccat
    11341 atcaatttcc tgaagtgcct tgagatgaga atgaaaggac gcagacaaag gcccccgttc
    11401 ttcgggatgg acaaagtcaa aaaaactttt tcccagcatg tcggcagccc catagcccag
    11461 gaccttgaga caatttggtg tgacgtactg acattcaccc gtcgcggtgg tttcaatgat
    11521 caagtcatag gcatgttcca cgatggctcg atagcgctgc tcactatccc gcaaggcatc
    11581 ttcggctttt ttacgttgga gttcacc
//
LOCUS       DAORAQ010000077        11456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_929_length_11456_cov_69.132006, whole genome shotgun sequence.
ACCESSION   DAORAQ010000077 DAORAQ010000000
VERSION     DAORAQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11456
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_929_length_11456_cov_69.132006"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKM72_08400"
     CDS             <1..386
                     /locus_tag="PKM72_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60844.1"
                     /translation="SRIENLNKKLGTRLLATQLVAKDLPDIFTRKVGSFQLIGKTRPV
                     VVYELLGRESEVDEICRKAIQKFLHGVGCFYTRRWDQAEESFRTCLQLRGRDGPSHFY
                     LSLCSHYRNHPPSPDWQGTIILTTK"
     gene            749..1180
                     /locus_tag="PKM72_08405"
     CDS             749..1180
                     /locus_tag="PKM72_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60845.1"
                     /translation="MAETTAAMFGGHKTNGLVLVVDDEPDVRKVVRMTLEKAGYDVIE
                     AEDGEKAIQEVQKGENPLMLDVIISDIRMPKINGVEAINYFQQQYPRVPLIVLTGFPD
                     MEMATGFLKQGIVDYLVKPVEKEKLIKSVATAMEKRDLNRL"
     gene            1389..2450
                     /locus_tag="PKM72_08410"
     CDS             1389..2450
                     /locus_tag="PKM72_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNP60846.1"
                     /translation="MSTVDPPITVAILGAGKGGTAMLESFLNLPHIRLLGIADTNPHA
                     LGFQLARLHNIPTVSHPLKLIQEDKLNLIIDVTGDPILPSYITEHKHSGAEVLGGTAA
                     KVLSELIQHQTFIQAQLFHSEKLASMGTFASGIAHDINNPLYVILAMAEEIQEENNLD
                     RIRQHAASIHEAAQRIQAISRNITDYVRTSSHQELEQIDLHARLDEALSISRFATKFR
                     EITVQKHYQNGLTVQAKPEEILQVFINLITNAIHAMEEKGSLTITTSHNNGTVQIAIS
                     DTGCGISPEHLKKIFNPFFSTKPKGQGTGLGLYNVKTIVKKYHGDLEVESEPGKGTTF
                     RLSFPVISPAENMVSREKP"
     gene            2437..4902
                     /locus_tag="PKM72_08415"
     CDS             2437..4902
                     /locus_tag="PKM72_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60847.1"
                     /translation="MKSPEPAKRKKGLTKKLVLSMLLVGALPLVIGLVLAFYQGTQEI
                     REVNGSSFEALATETARKLDLVMADELARTALLTTDIKIIEQLERTRDALSELNPQEL
                     ALTLKQEQEAWNSKEADLLERILKGPFAGILQQHVGGTFMDPGHPIPVITRSATRGLF
                     ITDRAGRVVASLDSDIPYLHQDETWWKGAFHNGVGQPYIGQVAFDSLRGVYTFTIALP
                     IMDSLRYEAIGVLLRVYDAKEFFAPSIDIIRFGKTGHVMLIDSRGVVLSCPILPTGTS
                     ISDTRLIPLVTPLHAGWTLAPSDGHGGTATSIIGFAPLSNTRQITQISTGQTWHMFVW
                     QSSEEVFAPIEHFFKWIATFGLLGVGLLVTLGYIAASRIAAPIRRLQEAAKQIGRGEY
                     REPLTLSTGDEIEELADEVNRMNQQLMSQFAGLESQVEMKTQEVKYLEESTIKILDNV
                     PDPVLMVGPDEQIEYLNNASKEAFAVANGEMIGSPLFQIFSLDPATQDRLKQEMHAVQ
                     ITEPPSSDNTKTNPSNASLQDPLVPTNWTQTGTHRQEIRINQRTFRYRWFGVQARPGR
                     GPSVGLVLRDTTEESILQDRVIQGEKLASLGVLSAGIGHELNNPLVGVIGLGEAIQEE
                     TNPEQIKEYARGIVQHGKRMASIVRDFTGQSGKKLTEGQSLINLNELLEQSLTTVKDL
                     FPSIPVNIQTHLGSIPSIQGKALELGQAFTNIITNAFQSMKQGGKLDISTETSGHELE
                     IRIQDTGGGIPPTHLPRVFDPFFTTKGQGEGSGLGLTVAYRIINKLGGHIRMESEEGR
                     GTTCLITLPVSNAHLAKERNSTS"
     gene            4899..5498
                     /locus_tag="PKM72_08420"
     CDS             4899..5498
                     /locus_tag="PKM72_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNP60848.1"
                     /translation="MTSVRSSIGLSMVFLSTLLIGIFSSSFLQAGESNAMKNLSPEKV
                     ADFIHAIVEADRHVYTSHIVKRMQEQGVVVAREDWENKNAIPLPAQFLHISSKLVAES
                     GHGIRFRLISLWPIYRRNGPATDFERKALEQLSLNSDTPQRGIVSTGKKRLFQAIYAD
                     KAINDTCTTCHNAHPLSPKRDFSKGDVMGAIVITIPLED"
     gene            5609..7102
                     /locus_tag="PKM72_08425"
     CDS             5609..7102
                     /locus_tag="PKM72_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNP60849.1"
                     /translation="MLRFLIIQILIGLALTAPALGFDNQFSSSPSIPMDIGNIIQDIR
                     ELSHPSYQGRQAGTDGSLQSANYLVDRFESLGLQPGNHFSEQEQDFSWLQQRSLTAVQ
                     LLEPATITLSLVDSSQSSMTMSLVPGQEFLPILDSPSTRTTGKVVFVGYGIVDPARGI
                     DEYQGMNVDNRIVLFLRGKPPTYPKWVTHEEKAAIAKEKGAIGYLTATGPLLGRYEAR
                     KGLGQGPLAIYGEKPDNRPIPGAWIHGQILDQTLNAANASLEALQQAANDIGTFHSRP
                     LPLLAQFHWESHSLPGTLTNVIGILPGRDQQLRNELILIGAHRDHFGQQTGLLFPGAD
                     DNASGTAVMLELARMLSQSTTAPKRTILFVSFDGEERGLLGSTLYVSHPAFPLDRTVA
                     MINLDHLGAGDRKLTVGVTRMEKTMAQQAANLAGLQDTIQMYGYFPGGDHVPFFEAGV
                     PTVTVVSSGRHPHFHQPSDTLESIQPENLTIATKFLYSLISLLADNP"
     gene            complement(7317..7958)
                     /locus_tag="PKM72_08430"
     CDS             complement(7317..7958)
                     /locus_tag="PKM72_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNP60850.1"
                     /translation="MMRKVSKVFSLIGLVLIISFGLVSASFAEMAKWKLDHDHSRVGF
                     QVAHMVVSKTNGRFTEYSGVVEMDPEAKEFKTIEAVIQTASVTTDHQKRDEHLRSPDF
                     FDVEKFPTMTYAMKSYKKTGEDYTAIGELTLLGVTKEITLVGTFNGVAQDPWGNTRAG
                     FTASGTVNRKDFGMKFSKLLDSGGMLVGDEVKIILEIEVIKEKTVEKVKQEKE"
     gene            complement(8068..8724)
                     /locus_tag="PKM72_08435"
     CDS             complement(8068..8724)
                     /locus_tag="PKM72_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNP60851.1"
                     /translation="MTVKSIIYVVDPMCSWCWGFSPVIEEIVRRYRDQVTIEVLLGGL
                     RPGNTERFDERKRAYILSHWRAVHERTGQPFNFNFHPEPSFIYDTEPSSRAMIVVRQL
                     AQDREFMFLSSVQEAFYVKHMDITHEGVLADLASRQGIDRDRFLQGFNDSVIKQSVWQ
                     EFDRARQLGVSGFPALLGQNKTDHISFTHGYQSTEVLVPIIDEWMAEPFSGFSRSSKV
                     "
     gene            9036..10973
                     /locus_tag="PKM72_08440"
     CDS             9036..10973
                     /locus_tag="PKM72_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP60852.1"
                     /translation="MPGSPHYPLTNLDEETALRTILEGTATATGERFFEALVENLAKA
                     LHTDAAWVTEYFPDSRRLKALAFFLDGRFLKNWEMDIAGTPCEHVIEQARLIHFPDNL
                     LNLFPIDPDITGIKAASYMGLPLTDSVGNILGHLAVMDTRPMPAEPRVQTIFRIFASR
                     ASAELQRLQAESATREREQKLRRLVDSAMDAIIELDEDLRVTRINPAAEKVFQCKATH
                     LFGKNFSHSLAQKDPEKLRLFIQDLNTKPAGQRSLWISGGFTALRLDGSEFPAEATLS
                     QFHIEGRVYYTLILRNVNERLEAEQKIRSLTIEKDFLKEELHELQHFGEILGNSPALV
                     HVLRDIQQVADTEATVLISGETGTGKEVMARAIHANSRRRNHPFIKVNCAAIPATLIE
                     SEFFGHEQGAFTGATKKREGRFSLADGGTIFLDEIGELPLDLQGKLLRVLQEGEFDPV
                     GSSHTKKVNVRVLAATNRDLQKEVQDGKFREDLYYRLNVFPIHLPPLRERGEDVVLLA
                     KTFVQNFTQRMGRTVAPLSPDALRRLTAYHWPGNVRELQNVIERAVITAQNSQLNLDR
                     ALPDVEAKLSSSPETLTQEPSTCIRTLQELQNLERQNILLALEQSGWKVSGEQGAAKL
                     LGMNASTLASRMKALGITRAK"
     gene            11261..>11456
                     /locus_tag="PKM72_08445"
     CDS             11261..>11456
                     /locus_tag="PKM72_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60853.1"
                     /translation="MMSQDERDSNSQTPAEKRNLQDMCRQADQLFFHMLFHTQPGGCA
                     MSSVATTSTINGVNVEQLGAN"
BASE COUNT         3087 a         2989 c         2565 g         2815 t
ORIGIN      
        1 cctcccggat tgaaaacctc aataaaaagc tgggcactcg gctcctggct actcaactcg
       61 tcgcaaaaga cttacctgat atcttcaccc gaaaggtagg gagttttcaa ttaattggga
      121 aaaccaggcc ggtggtggtt tatgaacttc tgggtcggga atccgaagtg gatgagattt
      181 gccggaaagc aattcaaaaa tttttacacg gagtaggatg tttttatacc cggcgctggg
      241 accaagccga ggaatcgttt cgaacatgct tgcaacttcg ggggagagat gggcccagtc
      301 atttttactt gagcctatgc agtcattatc gaaaccatcc tccgtccccg gattggcagg
      361 gaacgatcat cttgactacg aagtaaccac actcttgaac ccacatgatt ttccgtcaac
      421 cctccagccc gcgatctatt ggcgaccgct gatacgttca aaaatgcaaa tgcagtatca
      481 acgggcatta ggagcctacc catgggccat accaggccaa tagtttcatc cttcggccgg
      541 aatgggcaca gttaaaagga ctccatgacc tctcaatcca ttgcttttgg tcaggagaag
      601 gaacatttcc tctttttatt attcaattga ttaagctaga atatttccat aatggaggcg
      661 gagagctaga gacattaaaa atgttcttga ccatacttgg tgggataccc attcgagttt
      721 tcaacctcac aacctaagga gatgcattat ggcggaaaca actgcagcaa tgtttggcgg
      781 acacaaaacc aacggcctgg ttctggtcgt ggacgatgag cccgatgtcc gaaaagttgt
      841 acggatgact ctcgaaaagg ccggatatga tgtcattgag gctgaggatg gggaaaaggc
      901 aattcaggaa gtgcagaaag gcgaaaaccc cttaatgttg gacgtcatca tttccgatat
      961 tcgcatgccg aaaatcaacg gcgtggaagc cattaactac tttcagcaac aatacccgcg
     1021 cgttccactc atcgtactca cgggattccc ggacatggaa atggccacgg ggtttctgaa
     1081 gcaagggatc gtggattatc tcgtcaaacc ggttgaaaag gaaaaattaa ttaaatccgt
     1141 ggccactgcc atggaaaaac gagatctcaa tcgtttgtaa tctgacggga ctgcctgttc
     1201 agattccgag gttcttctca cgggagaatg attccccttg aagaagatta tggtatatca
     1261 agtaaggccc tatcctcagg atagggcttt ataccatata gacttttcag catggagaaa
     1321 catctcttgc tccgcattcg accctagcct cgcctcattc tcgactctca caatcaagga
     1381 gggattccat gtcaaccgtt gatcctccca ttaccgttgc cattctcggg gccggcaaag
     1441 ggggaacggc catgttggaa tcctttctta atttacccca tatccgccta ttgggcattg
     1501 ccgacaccaa tcctcacgcc ctgggctttc aactggccag acttcacaat atcccgactg
     1561 tctcccaccc tctgaagctc attcaagaag acaagttgaa tttgatcatc gacgtgaccg
     1621 gcgatcccat acttccttca tacattacgg agcacaaaca cagcggagct gaagtgctcg
     1681 gaggaaccgc cgccaaagtt ctctcagagt tgatccaaca tcaaaccttt atccaagccc
     1741 aactgtttca ttcggaaaaa ctagccagca tgggaacttt tgcctcaggg attgcccatg
     1801 acattaataa cccgctgtat gtcattttgg cgatggccga agaaattcaa gaggaaaaca
     1861 atctcgatcg aattcgacaa catgccgcca gcatccacga ggcggcacaa cgcattcaag
     1921 ccatttcacg aaatattacg gactatgttc gcacatccag tcatcaggaa ctcgaacaaa
     1981 tcgacctcca tgcaagactg gatgaagcct taagcatttc caggtttgcg acaaaatttc
     2041 gagaaattac ggttcagaaa cattaccaaa acggcttgac tgtccaggcg aaacccgaag
     2101 aaattctcca ggtattcatc aatctgatta ccaacgccat ccatgccatg gaagaaaaag
     2161 gcagcctcac cattaccacc tcacataaca atggaacagt tcagattgcc atcagcgata
     2221 ctggctgtgg catttccccc gaacacctca aaaaaatttt caatcccttc ttctcaacca
     2281 aacctaaggg ccaagggaca ggcctcggac tgtataacgt taaaacgatt gtcaagaagt
     2341 accatggcga ccttgaagtc gaaagtgaac cagggaaagg caccacgttc cgattgtcct
     2401 tccccgtcat ttctcctgca gaaaatatgg tgtctcgtga aaagccctga acccgccaaa
     2461 cgaaaaaaag gactgaccaa aaaactggtc ctgtccatgt tgctggtcgg tgccctccct
     2521 ttggtcattg gactcgtgct ggctttctac cagggcaccc aggaaattcg cgaggtgaat
     2581 ggatccagct ttgaagcgct cgcaaccgaa acagccagaa agcttgatct agtcatggct
     2641 gatgaactcg cccgcaccgc cctactgacc actgatatca aaatcattga acaactcgaa
     2701 cgcacccgcg atgccctcag tgagttgaat cctcaagagc tggctctcac cctcaaacag
     2761 gaacaagaag cctggaacag caaggaggcg gacctccttg aaagaattct gaaagggccg
     2821 tttgccggga ttctgcaaca acatgtgggg ggaaccttta tggacccggg ccacccgatt
     2881 ccggtcatta cccgatctgc cacccgtggc ctatttatta ccgatcgcgc gggacgggtc
     2941 gttgcaagcc tggattccga cattccttac cttcatcagg atgaaacatg gtggaaaggg
     3001 gcatttcaca atggcgtcgg ccaaccgtat atcggccagg tggcgtttga ttcacttcgg
     3061 ggggtttata cgtttaccat tgcgctcccc attatggata gcttgcggta tgaagccatc
     3121 ggcgtcctac tccgtgttta tgacgccaag gagttctttg ctccttcaat cgacattatt
     3181 cgatttggta aaaccggcca tgtcatgctc attgacagcc gtggcgtggt cttgagctgc
     3241 ccgatccttc ccacgggcac ttccattagt gacacccgac ttattcccct tgtcaccccg
     3301 ctgcatgccg gatggactct agccccaagc gatgggcatg ggggtactgc aacgtccatc
     3361 atcggatttg cccccctgag caatactcgt cagattactc aaatatccac cgggcagacc
     3421 tggcatatgt ttgtctggca atcatctgaa gaggtctttg cccctataga gcattttttt
     3481 aaatggatag cgacttttgg gttactggga gtcgggttgc tcgtcacgtt gggatatatc
     3541 gcggctagtc gaattgccgc acccattcgc cgtcttcagg aagccgccaa acaaatcggc
     3601 aggggggaat atcgcgagcc ccttactttg tccaccggag atgaaattga ggaattggcc
     3661 gatgaagtca atcgcatgaa ccaacaattg atgtcccagt tcgccggatt ggaaagccaa
     3721 gttgagatga aaacccagga agtaaaatac cttgaggagt cgaccattaa aattttggat
     3781 aatgtgcctg acccggtcct catggtcgga ccagatgaac aaatcgaata tttaaataac
     3841 gcctcgaaag aagcctttgc cgtcgccaat ggcgagatga tcggttcccc actgtttcaa
     3901 attttctcat tggatcccgc aacacaagac cggcttaaac aggaaatgca cgctgtccaa
     3961 ataacggaac ctccatcttc agacaataca aagaccaatc cgtctaacgc ctcgttgcaa
     4021 gaccccttgg ttcccaccaa ttggacccaa acagggacgc atcgccagga aattcgaatc
     4081 aatcagcgaa cgttccggta ccggtggttt ggtgtccagg cccgaccggg aagggggccg
     4141 agtgtcggat tggtattacg agatacaacg gaggaaagca ttcttcagga cagggtcatc
     4201 caaggggaaa aattggcaag tttgggggtt ctaagcgccg ggattggaca tgagctcaat
     4261 aatccgttag tcggagtcat tggattaggc gaagcaatac aagaagagac taatcccgaa
     4321 caaatcaaag aatatgccag gggcatcgta caacatggaa aacgtatggc ttccattgtt
     4381 cgggacttta ccggtcaatc cgggaaaaaa ttgaccgaag ggcaatcgct cattaacctc
     4441 aatgaactgt tggaacaatc tctgaccacg gtcaaggatc tcttcccatc aatcccggtt
     4501 aatattcaga ctcacctggg gtccatccca tccattcaag gaaaagccct ggaactcgga
     4561 caagccttta ccaacattat caccaatgct tttcaatcga tgaaacaagg tgggaagctt
     4621 gatatttcaa cagaaacgtc aggacacgaa ctggaaatcc gcattcaaga cacgggcggc
     4681 ggtatccctc caacgcatct accaagagtc tttgatcctt tttttaccac gaaaggccag
     4741 ggagaaggat cgggccttgg actcaccgtt gcctatcgga ttatcaataa attaggcgga
     4801 catattcgaa tggaaagtga agagggccgg ggtacgacct gtttaattac cttacccgtc
     4861 tcgaatgccc acctggcaaa ggaaaggaat tcgacgtcat gacaagcgta cgaagtagca
     4921 tcggtttatc gatggttttt ctttcgaccc tcctcattgg aatattttct tcatcatttt
     4981 tgcaagcagg tgaatccaac gccatgaaaa atctgtcacc ggagaaagtg gcagacttta
     5041 tccatgccat cgtggaggcg gaccgccatg tctacacttc acatatcgtg aagagaatgc
     5101 aggagcaagg cgtcgtggtg gcgcgggaag attgggaaaa caaaaatgcc attccccttc
     5161 ctgcccagtt cctccatatt tccagtaaat tagtcgcgga atcaggtcac ggcattcgat
     5221 ttcgtttaat tagtctgtgg ccaatatatc gaaggaatgg ccctgcaacg gatttcgaac
     5281 gaaaggcctt ggaacaactt tcgctaaatt ccgatacacc tcaacgtgga attgtttcca
     5341 ccgggaaaaa gcgcttattt caggcaattt atgccgacaa agcgattaat gatacctgca
     5401 cgacgtgcca taatgctcac cccttaagtc ccaagcgtga cttcagcaaa ggcgacgtca
     5461 tgggggcaat tgtcatcacc attcctttag aagattaaag tccggcacac gtgctgcagc
     5521 agggtccata ttccacacag ccatttggac ggaacctctt ttacacgtat tgtgtcgtcc
     5581 cccgtaaatt ccggagaaag gtaggaactt gttacgattt ctcattatcc aaattttgat
     5641 tggattagcc cttacggctc cggctttggg ctttgacaat caattctctt cttctccatc
     5701 cattcccatg gatattggga atatcattca ggacattcgc gaactgagtc atccatcgta
     5761 tcagggtcgg caggccggca cggatggtag cttacagtcg gccaattatc tggtcgatcg
     5821 attcgaatct ctcggcttac aaccgggcaa ccacttctcc gaacaagaac aggatttcag
     5881 ttggttacaa caacggtccc tgacagcggt tcaattgctt gagccggcga caatcaccct
     5941 ttctctggtt gactcaagcc aatcgtcaat gacgatgtcg cttgtcccag ggcaggagtt
     6001 tcttccaatc ttggattctc cttccaccag aaccacaggc aaagttgtct ttgtgggata
     6061 cggaattgtt gaccctgccc gcggaataga tgagtaccag ggaatgaatg tcgacaatcg
     6121 catcgtgcta tttcttcggg gtaaaccccc tacctacccc aagtgggtca cgcatgaaga
     6181 aaaagcggca atcgccaaag aaaaaggagc gatagggtat ttaacggcaa ccggcccact
     6241 gcttggtcgt tatgaagccc gcaaaggtct gggacaaggt cctctggcca tctacggaga
     6301 gaaacccgat aaccgcccca ttcctggggc atggatacat gggcaaattc ttgatcagac
     6361 cttgaacgcg gcaaatgcct cccttgaagc tctgcaacaa gccgccaacg atatcgggac
     6421 atttcatagc agaccacttc cccttctggc gcaattccac tgggaaagcc acagcctgcc
     6481 cggaacgcta acgaatgtca tcgggatatt accgggtcgg gaccagcaac tacgcaacga
     6541 actcattctc atcggtgcac atcgcgacca ctttggtcaa caaaccggcc tgctttttcc
     6601 tggagccgat gacaatgcat ctgggacagc cgtgatgttg gaactggcca gaatgctctc
     6661 ccaaagcaca accgcaccca aacgaaccat tctcttcgtg tcttttgacg gagaagaacg
     6721 aggcctattg ggatcgactc tctatgtcag ccatccggca tttccattgg atcgaacagt
     6781 cgccatgatt aatctcgatc atctgggagc gggtgataga aaactgactg ttggagtcac
     6841 tcgaatggaa aagacgatgg ctcagcaagc agccaacctg gcgggattac aggacacaat
     6901 tcaaatgtat ggatatttcc ccgggggaga tcatgtccca ttttttgagg cgggggtgcc
     6961 gaccgtcaca gtcgtcagca gtggcagaca cccccatttt catcaacctt ctgataccct
     7021 agagtccatt caacctgaaa acttgaccat tgctaccaaa tttctctact cactgatttc
     7081 cctgttagcg gacaatcctt aaaatagcct gtcatcctag gctttaccgc gaaggacctg
     7141 tgttagggtg aggatatagc cattgccaaa tcctttgggg aaaatgttct gaggttgccg
     7201 attggcttac catgaagcct cacccaaccc taccttcatg atttcaccgc tcggatttcc
     7261 tcagctatac ggaaagggaa aatgcataaa tttaccacac atgtccccct gcccttttat
     7321 tctttttctt gtttcacttt ttcgacggtt ttttctttga tcacctcaat ttccaatatg
     7381 attttgacct catcacccac aagcattcct ccactatcca ataacttgct gaatttcata
     7441 ccgaaatctt ttcggtttac cgttccggaa gcggtgaagc cagcccgtgt attgccccaa
     7501 ggatcctgcg cgaccccatt aaacgttccg actaacgtga tctctttagt gactcccaat
     7561 agagtcaact ctccgatggc ggtgtagtcc tctccggttt ttttataact tttcatggca
     7621 tacgtcatgg tcggaaactt ctcgacatca aaaaagtccg ggcttcgtaa atgctcatca
     7681 cgtttttgat ggtcggtcgt gaccgaggcg gtttggatga ctgcttcaat ggttttaaat
     7741 tctttggcct ccggatccat ttccacaact cctgaatact cggtaaacct gccattggtt
     7801 ttggatacca ccatatgggc aacttgaaat ccaaccctgg aatggtcatg atccagtttc
     7861 catttagcca tttccgcaaa cgaagcagaa acaagcccaa aactaatgat cagaactaag
     7921 ccaatcaaag aaaacacttt cgaaaccttc cgcatcatgt aacctcctgt gaaaattgaa
     7981 gacatacccg ttgtatggtc ttcgttgatt gataaacaaa aacccatcat ctaaaagtca
     8041 tccatcattc tattctcatg caatgatcta gactttcgat gaacgggaga agccagaaaa
     8101 tggctcggcc atccattcgt caatgatagg aaccaacacc tctgtggatt gatacccatg
     8161 cgtaaaactg atgtggtcag ttttattttg accaagcaac gcaggaaaac cactcactcc
     8221 caattggcga gcccgatcaa actcctgcca gaccgattgc ttaatcacag aatcattgaa
     8281 cccttgcaaa aatctgtcgc ggtcaatacc ttgacggctg gcaagatccg ccaaaactcc
     8341 ttcatgcgta atatccatgt gcttcacgta aaatgcttcc tgcacagaac tcaaaaacat
     8401 aaactcccta tcctgagcca attgtcgcac cactatcatc gctcgggatg atggttcggt
     8461 gtcatagatg aatgaaggct ccggatgaaa attgaagtta aatggctggc cggttctttc
     8521 atgcaccgca cgccaatggc tgaggatgta ggcacgtttt cgctcatcaa atcgttcggt
     8581 atttccgggg cgcaaaccac ccaacaagac ttcgatcgtg acctgatctc gatagcgccg
     8641 aacgatctcc tcaataaccg gggaaaatcc ccagcaccac gaacacatgg gatcaaccac
     8701 atagataatc gatttcacgg tcatgtctac cacctggctc cctcgattac ctcaccagac
     8761 atacccaaca aggcaatacc ctacaaatga aattagacta ctttatcaca ccggacatat
     8821 ggaacttgtc aatttccgat caaggtttct tttaaatttc atgtaggttc atgtgtgtct
     8881 gaaacaacac ctgcgaactc cctccccttc caaacctcat caaccgccct atctttgtat
     8941 gctgtaaaaa cccagaatcc tcaaacctat attagcccat tctttacacg ttaaaaacta
     9001 atggatcata atagggtttt tatttgggag gctttatgcc tggatcccca cactatccgc
     9061 taacaaacct cgacgaagag accgcactcc gaactattct ggaagggacg gccaccgcca
     9121 ccggggaacg attttttgaa gcgcttgtgg aaaatctcgc caaagccctg cacaccgatg
     9181 ccgcctgggt cacggaatat ttccccgatt cccgacgatt gaaagccctg gcgttttttt
     9241 tagatggcag gttcctcaaa aattgggaaa tggatattgc cggtaccccg tgcgagcacg
     9301 ttatcgagca agcccggctc attcattttc cagacaactt attgaatctc tttccaattg
     9361 atccggatat caccggcatc aaggcagcca gctatatggg tctcccgctt accgattcgg
     9421 tcggaaatat tcttggacat ctggcggtca tggataccag acccatgccg gcggaaccgc
     9481 gggtgcagac gatttttcga atttttgcat caagagcgag cgccgaactc cagcgcctcc
     9541 aagcagaatc cgcgacacgt gaacgtgaac aaaagttgcg gcgattggtc gacagtgcaa
     9601 tggatgccat cattgaatta gacgaggatc ttcgagtcac ccgaattaat ccggctgccg
     9661 agaaggtgtt tcaatgtaaa gccacccatt tgtttggtaa gaatttttcc cactctcttg
     9721 cccaaaagga ccctgagaaa ttacggcttt ttatccagga tctcaacacg aaacctgcag
     9781 gccagcgatc tctctggatt tccggaggct tcacggctct ccgcctggac ggctcggaat
     9841 ttcccgcgga agccacgctt tcccaatttc atatagaggg aagggtctac tacaccctga
     9901 ttcttcgtaa tgtgaatgaa cgcctggaag cggaacaaaa aattcggtcc ctcacgattg
     9961 aaaaagattt cctcaaagaa gaattacatg agctccaaca ttttggggaa attttgggga
    10021 atagtcccgc cttggtgcac gtcctccggg acatccaaca ggtcgcggat accgaggcca
    10081 ccgtgctcat ttcaggcgaa accggaaccg gcaaggaggt gatggcccgc gccattcatg
    10141 ccaacagccg gcgtcggaat cacccattca tcaaagtaaa ttgcgcggcc attcctgcta
    10201 ccttaatcga aagtgaattt tttggacatg aacagggtgc atttaccggc gccacgaaaa
    10261 agcgggaagg acggttttca ttggccgacg gagggaccat ttttctggat gaaatcggcg
    10321 aattaccttt ggatcttcaa gggaaactgt tacgcgttct tcaggaaggg gaattcgatc
    10381 cggtcggcag ttcccacacc aaaaaggtaa atgtccgtgt cctggcagcc acaaaccggg
    10441 atttacagaa ggaggtccaa gacggaaaat ttcgtgaaga tctttattat cgattgaatg
    10501 tctttcccat ccatcttccg cctttgcggg aacgcggaga ggatgtggtc ctcctagcca
    10561 agacatttgt tcagaatttt actcaacgaa tgggtcggac ggtggccccc ctttcccctg
    10621 acgcactccg acgcctcacg gcttaccatt ggccgggaaa tgtgcgggaa ttgcaaaacg
    10681 tgatcgagcg ggctgtcatt acggcccaaa acagtcaatt aaatcttgac cgtgccctcc
    10741 ctgatgttga agcgaaactc tcctcttctc cagaaactct tacacaggag ccatccacct
    10801 gcattcgcac tcttcaggaa cttcagaatt tggagcggca aaacattctt ctagctctgg
    10861 aacaaagcgg ctggaaagta tccggtgaac aaggagcggc caaactactg ggcatgaatg
    10921 cttcaaccct tgcatctcgt atgaaagctt tgggcattac tcgcgcgaaa taattcctgc
    10981 acgatttcta cccatatttt ttcaagtctt tccgattctc tttcagcatt tcttcatttt
    11041 caggattcta tcctatttgc gaactttcgc aaattcgtct tatgaaattt cgcaattcgg
    11101 caaatttcgt gagcgatttc cacgcttcat aaaatttcaa catattgttt ttattgtttt
    11161 ttttattaac aatttaagct tggcacaccc cctgcaaaat acctttggca tggaagacag
    11221 cacatatcaa ccaggttctc aaggaacagg caggaacacc atgatgagtc aggatgagcg
    11281 agattcaaac tctcagacgc cagctgaaaa gcggaacctt caggatatgt gccgtcaggc
    11341 ggatcaactt ttttttcaca tgctgtttca cactcaacca ggaggatgtg ccatgtcatc
    11401 tgtcgccaca acatcaacca tcaatggggt caacgtcgaa caattagggg ccaata
//
LOCUS       DAORAQ010000078        11337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_938_length_11337_cov_68.341340, whole genome shotgun sequence.
ACCESSION   DAORAQ010000078 DAORAQ010000000
VERSION     DAORAQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_11337_cov_68.341340"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3815
                     /gene="rpoB"
                     /locus_tag="PKM72_08450"
     CDS             <1..3815
                     /gene="rpoB"
                     /locus_tag="PKM72_08450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNP60854.1"
                     /translation="GLHAAFKSVFPIMDYNNSAILEFSSYSLGTPKYDTRECIEQGMT
                     FAAPLKLRVRLVVFDQQDKSVKNRVLDVREQEVYVGELPLMTDRGTFMVNGTERVAVS
                     QLHRSPGPSFGHDKGRTHSSGKVLFSGRIIPYRGSWLDFEYDAKDILYVRIDRRRKMP
                     ATILLKGFGFSTDDLLKMYYPVEEVRVDNGALLRKLDPDIHAGLRSSSDLFEKGSGEL
                     LVKEGAKLNKTLMGRIRSAGIKEIPIKQEDLVGRAVLTELVDPGTQEVILEKNQRLTA
                     PVLERILESGVEGFKVIYLDSPTTSPVILDTLEAERTNSKEEAWVEIYKRLRPGEMPS
                     IESAKLLFENLFLNPKRYDLSPVGRLKMNKRFNLDLSMEQRTLSAQDIVEVVRCLVDL
                     KAGKGAVDDIDHLGNRRVRSVGELLENQIRIGLARMERSIKERMNLLDMETVLPHDLI
                     NAKPIVGAIKEFFAGSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVH
                     PSHYSRICPIETPEGPNIGLITSLATYARINEFGFIEAPFRKVKKGRVTDDVEFLSPL
                     DGDQVVIAQANSVMDKDGTLTSETITAREAGEFVTTTPDKVDYLDVSPKQVVSVATAL
                     IPFLEHDDANRALMGSNMQRQAVPTIKSEAPLVGTGMEAVVARDSGYLAIAQRDGIVE
                     SVDARRIVVRTGLKEKDDKKKGSRILDTYDLIKFQRTNQNTCITQTPIVRIGQPVKQG
                     QVLADGPAMDRGELALGKNVLVAFMPWGGYNYEDAILLSEKMVKEDVFTSIHIEEFEV
                     EARDTKLGKEEITRDIPNLGEEALRNLDESGIVRIGAEVKPGDILVGKVTPKGETQLT
                     PEEKLLRAIFGEKAGDVKDTSLQVPPGIEGIVVDVKIFSRKGVDKDERSRTIETDDRV
                     KVERDYQEELRIIEEERNRKIRKLLLGQFVGRDLMDPDSGEVILKKKGRITAEVLRKL
                     SDDDARRVILADAEEQKKLDEIEREAKDQIEILQTQYDEKVGRLRRGDELPPGVIKLV
                     KVYLAIKRKIAVGDKMAGRHGNKGVVSRIMAEEDMPYLPDGTPVQIVLNPLGVPSRMN
                     VGQILETHLGWAAKALGIQVETPVFDGASEREIKELLKKAELPESGQSVVYDGRTGEP
                     FKSPVTVGYMYMLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAL
                     QAYGAASTLQEFLTVKSDDVPGRSRMYEAIVKGENFLEPGLPESFNVLVKELQSLGLD
                     VELIKSAD"
     gene            3869..8023
                     /gene="rpoC"
                     /locus_tag="PKM72_08455"
     CDS             3869..8023
                     /gene="rpoC"
                     /locus_tag="PKM72_08455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNP60855.1"
                     /translation="MDSIYSLFEKPRDAVSFDAMRIRIASPEKIRSWSYGEVKKPETI
                     NYRSFKPERDGLFCAKIFGPTKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERM
                     GHIELAAPVAHIWFLKGVPSRIGILLDMSLKQLEKILYFEAYVVLDPGNTNLKERELL
                     TEERYRECVEEYGANSFKVGIGAEAIRELLRKVDINALWDERHVKIKSTTSAAVGKKL
                     TKRLKVIEAFHKSGNNPEWMIMDAIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLVELKAPGVIIRNEMRMLQEAVDALFDNGRRGRVIRGANKRPLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELRLNQCGLPKKMALELFKPFIFHKLEERGAA
                     TTIKSAKRLVEKERPEVWDVLDEVIREHPVMLNRAPTLHRLGIQAFDPILVEGKAIRL
                     HPLVCAAFNADFDGDQMAVHVPLSVEAQIESRVLMMAANNVLSPANGRPLSIPSQDMV
                     LGCYWLTKDRDGARGEGKIFSSAEEVRIAYDSQEVEEQARVKVRIDGDMIDTTVGRVI
                     LSEVLPTSMPFSHVNQLMTKKELTKLVDRVYRKTGLHEVVVMLDKLKDLGFSYATKAG
                     VSICIDNMHIPSRKKELIERAKGDVYQVHEQYSEGLITNGERYNKVIDIWAHVGEEVA
                     IEMMKEIKEGGGRGAAEGLNPIYMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETP
                     ITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDAAQDVIISEPDCFT
                     TDGIIVSALVEGGEVIQSLDERILGRLTAEDLLDPVTQEVIVKAHEELEEELCKTIVE
                     AGIDHVKIRSVLTCQSRWGVCAKCYGRDLARGRLVEMGEPVGVIAAQSIGEPGTQLTM
                     RTFHIGGTASKVVEQTVLASKHDGTIKFLSFDAKKNADFYNPSMAVKTRQGDWVVMVR
                     NGKVAVIDETGREREKYPVVYGGKIKVPDGGKVEIGQKFVEWDPYSLTILTEVGGRVA
                     FGDIAEGVTMKEEVDEVTGLSRRVVIEQAGTNLRPRLSVKDESGKTAKLSSGSPARYL
                     LPVGAHIFVEKGSSVFPGDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEHAVVTE
                     IDGEVSFGGFVKGLRKVVVDNKIGDVKEYFIPRGRHVNVHEGDWVRAGEPLMDGSANP
                     HDILSVLGPRELQKYLVDEVQEVYRLQGVVINDKHIEVITRQMLKKVRIEDAGDSSFL
                     PGTQVNKSVFDDENERVLSEGGQPALGKPVLLGITKASLSTDSFISGASFQETTRVLT
                     EAAINGRTDDLRGLKENVIVGRLIPAGTGWGAYRETFVPKRTEEVSLSSEDQQGLNKV
                     HSLSDK"
     gene            8153..8524
                     /gene="rpsL"
                     /locus_tag="PKM72_08460"
     CDS             8153..8524
                     /gene="rpsL"
                     /locus_tag="PKM72_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNP60856.1"
                     /translation="MPTVNQLIRVGRKAIKIKSKSSALNQCPQRRGVCLRVYTTTPKK
                     PNSALRKVARVRLTTGLEVTAYIPGMGHNLQEHSIVLVRGGRVKDLPGVRYHMIRGAL
                     DAVGVANRKQGRSKYGAKRPK"
     gene            8588..9058
                     /gene="rpsG"
                     /locus_tag="PKM72_08465"
     CDS             8588..9058
                     /gene="rpsG"
                     /locus_tag="PKM72_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNP60857.1"
                     /translation="MPRRPLVLRQKVVQDARYKDQVVGRFLNILLQDGKKSTAERVCY
                     GAFDIVRDKTGDEPLKVFHAALGNVKPMVEVKSRRVGGASYQVPVEIRPVRQVALALR
                     WIKQSALARSGKSMREKLAAELMDAANNNGSAVKKRDETHRMAEANRAFAHYRW"
     gene            9156..11237
                     /gene="fusA"
                     /locus_tag="PKM72_08470"
     CDS             9156..11237
                     /gene="fusA"
                     /locus_tag="PKM72_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNP60858.1"
                     /translation="MSADFILSRTRNIGIMAHIDAGKTTTTERVLYYTGVSHKIGEVH
                     EGAATMDWMEQERERGITITSAATTCFWKESRINIIDTPGHVDFTIEVERSLRVLDGA
                     IAVFDSVQGVEPQSETVWRQADKYRVPRIVFMNKMDRVGADFFASVKSLVDRLGAKPV
                     PVQLPLGSEDGLRGVIDLLVMKALVFDDETLGAAYSVQEIPDEFMGVAKEYREKLLDA
                     VVEFDEVVMERYLAGEVLGVDEVKRVIRAGTIGLKINPVFCGSAFKNKGVQPLLDGVV
                     DYLPSPVDLPPVVGIDPHTEGEITRKPQDSEPFAALAFKIMSDPFSGQLTYFRVYSGK
                     LTAGSYVYNVAKGKKERVGRLLKMHANKREEIEEVSAGDIAAAVGLKDTRTGDTLCDE
                     KHQILLEVIKFPEPVISLAVEPKTKQDMDKLGYSLEKLAQEDPSFQVKSDDETGQTII
                     SGMGELHLEIIVDRLLREFKVQANVGKPQVAYRETIKGRAEAEGKYVKQTGGRGQYGH
                     VWLRVEPAESGVGLEFVNKIVGGTVPREYIAPVEKGVRERMESGILAGYPLRDLKVTL
                     FDGSFHEVDSSEMAFKIAGSMALVSACQKADLVLLEPVMKVEVLVPQDFMGDVIGDLN
                     SRRGKIHGMRARGTSQIVDAEVPLSEMFGYSTDLRSKTQGRATYSMEFESYEVVPKLM
                     AQQIIKKNQGE"
BASE COUNT         3002 a         2012 c         3288 g         3035 t
ORIGIN      
        1 agggattgca tgcagcgttt aaaagtgtgt ttcccattat ggattataac aattcggcca
       61 ttttagaatt ttccagctat tctttgggaa ctccaaagta tgacacgagg gaatgtattg
      121 agcagggaat gacctttgcc gcacccttaa agcttcgtgt gcgcctggtt gttttcgatc
      181 aacaagataa aagtgtgaaa aacagggtgt tggatgtgag ggagcaggaa gtctatgtcg
      241 gggagcttcc tttgatgacc gatcgcggca catttatggt gaatggcacg gagagggtgg
      301 cagttagtca attgcatcga tctccaggtc cttcattcgg ccatgataag ggccggacgc
      361 attcaagtgg caaagtcttg ttttctggtc ggattatccc ttatcgggga tcgtggcttg
      421 attttgaata tgatgccaaa gatattttgt atgtacgaat tgaccgccgc aggaaaatgc
      481 cggcgacaat tttattaaag ggttttggtt ttagcacgga tgatcttctt aagatgtatt
      541 atccggtaga ggaggtgcgc gtcgataatg gtgccttgct ccgcaagctt gatcccgaca
      601 ttcatgcagg attgagatct tcttcggacc tcttcgagaa ggggtccggg gaactattag
      661 tcaaagaagg tgccaagctc aataagacgc tcatggggcg tatccgatcg gctggaataa
      721 aagaaattcc tataaagcaa gaagatttgg ttggtcgagc cgtcttaacc gaactggtgg
      781 atcctggcac tcaggaggta attttagaaa aaaatcaacg actcacggca cccgtgttgg
      841 agcgaattct ggaaagcgga gttgaaggat ttaaagttat ttacctggat agccctacca
      901 ccagtcctgt cattctggat accttggagg ccgagcggac caattcaaag gaagaagcct
      961 gggttgaaat ttataaacgc cttcggcctg gggagatgcc atccattgag tcggcaaagt
     1021 tattgtttga aaatcttttt ctgaatccca agcgttatga cctttcccct gttggtcgtt
     1081 tgaaaatgaa caaacgattt aaccttgatt tgtcaatgga gcaaaggacg ctatccgccc
     1141 aagatattgt cgaggtggtg agatgtttag tggatctgaa ggccggaaag ggtgcagttg
     1201 atgatattga ccatctagga aatcggcgtg ttcgttcggt tggtgagttg ttggaaaatc
     1261 aaatccgtat tggtctggca agaatggagc gaagcattaa agagcgaatg aatctgttgg
     1321 atatggaaac cgtgctgcct catgacctga tcaatgccaa gcctatagtc ggtgccatta
     1381 aggaattttt tgccgggagt cagttgtcgc agtttatgga ccaaactaac ccgttggcag
     1441 agatcactca taaacgaaga ctgtctgctt tggggcctgg tggtttgaca agggagcgag
     1501 caggttttga ggtgcgggac gttcatccgt ctcattatag tcgtatttgt cctattgaaa
     1561 ctccggaagg accgaatatt gggttaatta cgtcattagc tacgtacgct cggattaatg
     1621 agtttggatt tattgaagct ccatttcgga aagttaaaaa aggtcgggta accgatgacg
     1681 ttgaattcct gtcgccattg gatggggatc aggttgttat cgctcaagcc aattcagtga
     1741 tggataagga tgggacgctc acgtctgaaa cgattacggc cagagaagca ggggaatttg
     1801 tgaccacgac ccccgataag gtggactatc tcgatgtttc tcctaagcag gtggtcagtg
     1861 tggcgactgc gttaattcca ttccttgagc atgatgacgc caaccgggcg cttatgggtt
     1921 cgaatatgca acgccaggca gtccccacaa ttaagagtga agcacctttg gtcggaacgg
     1981 gtatggaagc tgtcgtagca agagattctg gatatttagc tattgctcaa cgagatggga
     2041 ttgtagagtc ggtagatgcg agaagaattg ttgtaagaac cggactcaag gaaaaggatg
     2101 acaagaaaaa aggttcccgt attctggata cctatgattt gattaaattt caacggacga
     2161 accaaaatac gtgtatcacc caaaccccca ttgttcgaat cgggcagccg gtcaagcaag
     2221 gtcaagtgct agcggatggt ccagcgatgg atcgcggaga attggcgttg ggaaaaaatg
     2281 tgctcgtcgc ctttatgcca tggggtggct acaactatga ggatgctatt cttttaagtg
     2341 aaaaaatggt taaagaggat gtgtttactt ctatccatat cgaagagttc gaagtagagg
     2401 cgcgtgatac gaagttagga aaagaagaaa tcactcggga tattcctaat ctaggggaag
     2461 aggcccttcg aaatttggat gaaagtggaa tcgtgcgaat aggtgcagaa gtcaagcctg
     2521 gcgatattct tgttgggaaa gtcactccca aaggagagac gcagttgacc ccggaggaaa
     2581 aattgttgcg tgcgattttc ggggaaaagg ccggggacgt gaaggatact tcgcttcagg
     2641 taccaccagg cattgaaggt attgttgtgg acgtcaaaat tttctcgcga aaaggcgtag
     2701 ataaagatga acggtctcgg acaatcgaga cggacgatcg cgtcaaagtt gagcgggact
     2761 atcaggaaga attgcgaatt atcgaagaag agcgaaatcg taagattcga aagcttttgc
     2821 ttgggcaatt tgtcggtagg gatttaatgg atcctgatag tggagaagtg attctaaaga
     2881 aaaaggggcg aattacggct gaagtccttc gtaaattgtc tgatgatgat gcgcgtaggg
     2941 tgatactagc tgacgcggaa gaacagaaaa aacttgatga aattgagcgg gaagccaaag
     3001 accaaataga aatcttgcag acgcaatacg atgaaaaggt tggtcgtctt cgacgtggcg
     3061 atgaattgcc tcccggagtc attaagcttg taaaggtgta tttagcgatc aagagaaaaa
     3121 ttgccgttgg cgataaaatg gccggtcgcc atggaaataa gggcgtcgtt tccagaatta
     3181 tggcggaaga agatatgccg tatctcccgg atggaacccc tgttcaaatt gtcctgaatc
     3241 cgcttggtgt tccttcgcga atgaatgtag gacaaattct tgagacccat ttgggatggg
     3301 cggccaaggc tttagggatt caggttgaga cccctgtttt cgatggggca tcggaaaggg
     3361 agatcaagga gttactgaaa aaagcagagc tcccggaatc aggccaatcg gttgtgtatg
     3421 atggccggac aggagagcca tttaaaagtc ctgtcacggt aggatacatg tatatgttga
     3481 aactccacca tttggtggac gataagattc acgctcgctc aatagggccg tattctttgg
     3541 ttacccagca accgcttgga ggaaaggctc aatttggtgg tcaacgctta ggagaaatgg
     3601 aggtctgggc tcttcaggcg tatggggccg cttccacctt gcaggaattt ttgacggtaa
     3661 aatcggatga cgtacctgga cggtcacgaa tgtatgaagc gatcgtgaag ggagaaaact
     3721 tccttgagcc tggtcttccg gagtccttta atgtgttagt aaaggagttg cagagtttgg
     3781 ggttggatgt ggagctgatc aaatctgctg attagacggg tagtctgatt ctgcaatccc
     3841 atcattcaac ataatcggag gaacgaactt ggacagtatc tattcgcttt ttgaaaaacc
     3901 tcgtgatgca gtttcgtttg atgccatgcg gattcgtatc gcctcgcctg agaaaattcg
     3961 ctcgtggtcc tacggtgagg taaaaaagcc tgagacaatc aactatcggt cctttaagcc
     4021 tgagcgggat gggttgtttt gcgcaaaaat atttggacca acaaaagatt gggaatgtaa
     4081 ttgcggtaaa tacaagcgaa tgaagcatcg cggtattgta tgtgataaat gcggcgtgga
     4141 agtcattcag tcaaaagttc gacgtgagag gatgggccat atcgaattgg ctgctcctgt
     4201 agcccatatt tggtttctca agggtgtgcc aagccgaatt gggatccttt tggatatgag
     4261 tttaaagcag ctggaaaaga ttctgtattt tgaggcctac gtggtcttgg atcctggtaa
     4321 tacgaatttg aaagaacggg agctcctgac tgaggaaaga tatcgggaat gtgttgagga
     4381 gtatggggcc aattcattta aagtgggtat tggggctgaa gctattcgtg aattattgcg
     4441 caaagtagat ataaatgctt tgtgggatga gcgccatgtc aagattaaaa gtacgacatc
     4501 cgcggctgta ggaaaaaaac tgacgaaacg tttgaaggtc attgaggcat ttcataagtc
     4561 tggaaataac ccggaatgga tgattatgga tgccattcct gtgttgccgc ctgagcttcg
     4621 ccctttggtg cccttagatg gtggccggtt cgcaacgtcg gatcttaatg acctctaccg
     4681 acgagtgata aaccgcaata accgtcttaa gcgattggtg gaattgaagg cccctggtgt
     4741 cattatccgc aatgagatgc gtatgcttca ggaggcggtc gacgcgttgt ttgataacgg
     4801 ccgtcggggt cgggttattc gtggtgccaa taagcgtcct ttgaaatcct tgagcgatat
     4861 gcttaaagga aaacagggtc gatttaggca gaatctcctg gggaaacggg tggattactc
     4921 tggacgctcg gtgattgtgg ttggtcctga attgcggctt aaccaatgcg gattgcctaa
     4981 aaagatggct ctagaattat ttaagccgtt tatttttcat aaattggaag aacgtggtgc
     5041 ggcgaccaca atcaaaagtg ccaagcgatt ggttgagaag gagcgtccag aagtctggga
     5101 tgttcttgat gaggtaattc gggagcatcc ggtcatgttg aatcgtgctc caacgcttca
     5161 tcgactaggg attcaagcct ttgaccctat tttggttgaa ggtaaggcta ttcgtcttca
     5221 tccgttagtc tgtgcggcct ttaatgcaga ctttgacggg gatcaaatgg ctgtccacgt
     5281 tccgctttcg gtggaagccc aaattgaaag tcgggtatta atgatggctg ccaataatgt
     5341 cctttcacct gcaaatggcc gacccttgtc cattccatcc caggacatgg tgttagggtg
     5401 ctattggctg acgaaggatc gtgatggtgc ccgtggcgaa gggaaaattt tttcgtccgc
     5461 agaagaagtt cggattgcct atgattcgca ggaagtagaa gagcaggcga gagtgaaagt
     5521 gcgaatcgat ggagatatga tcgataccac ggttgggcga gtaattcttt cggaagtgct
     5581 gccaaccagt atgccatttt cacatgtgaa tcagcttatg acaaaaaaag agctgacgaa
     5641 attggttgat cgtgtttatc gcaaaaccgg tttacatgaa gtggtcgtca tgcttgataa
     5701 attgaaggat ttaggattct cttatgccac aaaagctggg gtttctattt gtatagacaa
     5761 tatgcatatt ccttctcgaa aaaaggaatt gatcgagcgg gcaaagggag atgtttatca
     5821 agttcatgag caatacagtg aaggcttgat tacaaatggg gagcgctaca ataaggttat
     5881 cgatatatgg gcgcatgttg gggaagaagt cgcaattgaa atgatgaagg aaattaaaga
     5941 gggaggcggg cgaggtgcag cagaaggcct taatcctatt tatatgatgg cggattcagg
     6001 cgccagggga agttcgcagc aaattcgcca attgggcgga atgcgcggtc ttatggccaa
     6061 gccttcaggg gaaattattg aaacccctat taccgctaat ttccgagagg gtctcactgt
     6121 tctgcagtat tttatttcga ctcatggggc acgcaaaggg ttggccgata cggcactcaa
     6181 gacggcaaat tccggatacc tcactaggcg ccttgtcgac gcggctcagg atgtgattat
     6241 cagtgagcct gattgtttta caacggacgg aatcattgtt tcggccttgg tagagggagg
     6301 agaagtgatt caatccctgg atgaacgcat ccttggtcgg ttgaccgctg aggatctgct
     6361 cgaccctgtc acgcaggaag tcattgttaa ggctcatgaa gagttagagg aagagctttg
     6421 taagaccatt gttgaagcag gcatcgatca cgtaaaaatt cggtcggttc tcacgtgcca
     6481 atccagatgg ggagtatgtg ctaaatgtta cggtagggat ttggcgcgcg gtcggcttgt
     6541 agaaatgggt gagcctgttg gggtcatcgc cgctcaatcc atcggagaac caggtactca
     6601 gttgacgatg cggaccttcc atattggagg cacggccagt aaggttgtgg agcaaactgt
     6661 tctagcatcg aagcatgacg gcacgatcaa atttttaagc tttgacgcaa agaaaaatgc
     6721 ggatttttac aatcccagca tggcggtgaa aacccgacaa ggagattggg tcgtcatggt
     6781 aagaaatggc aaggttgcgg taattgatga aactggccgt gagcgtgaaa aatatcctgt
     6841 tgtgtatggg ggtaagataa aggtccctga tggaggaaaa gtcgaaattg ggcagaagtt
     6901 tgtggagtgg gatccatatt ctctcacaat tctgactgaa gtggggggaa gggtggcttt
     6961 cggtgatatt gccgaaggag tcaccatgaa agaagaagtg gatgaggtca cgggtttatc
     7021 ccgtcgtgtg gtcatcgagc aagcggggac caatttgcgt ccacgcttat ctgttaagga
     7081 cgaatcaggg aagaccgcaa aactttcttc cggatcgcct gcccgatacc tcctgccggt
     7141 gggagctcat atttttgtgg aaaaaggctc gtctgtattt ccaggtgatg tgttggcaaa
     7201 aattccaaga gaaaccacta agacaaaaga tattaccgga gggttgcccc gagttgccga
     7261 attgtttgaa gcaagaaagc ccaaggagca tgccgtagtc acggaaattg atggagaggt
     7321 gtcttttggt ggttttgtga aaggcctgcg gaaggttgtt gtggataata agattggtga
     7381 tgtaaaagaa tactttattc cacgtggtcg acacgtcaat gtccatgaag gggattgggt
     7441 aagagctggg gagcccttaa tggatggatc tgctaatccg catgatattc taagcgtctt
     7501 gggaccaaga gaattgcaaa agtatttggt tgatgaagtg caagaggttt atcgcctaca
     7561 gggagtggtg attaatgata agcacattga ggtcatcacc cggcaaatgc tgaaaaaagt
     7621 gaggattgaa gatgctggcg atagttcctt cttgcctggc actcaggtca ataaatctgt
     7681 ttttgatgat gagaatgaga gggtcctttc tgagggtgga cagccggcat taggtaagcc
     7741 tgtcttgttg ggaatcacga aagcctctct gagtactgac agctttattt ctggtgcctc
     7801 tttccaggaa acaacaaggg ttctgacgga ggcagctatt aatggacgta cagatgattt
     7861 gcgtgggttg aaggagaatg tgatagtggg caggttaatt ccggctggaa cgggatgggg
     7921 ggcgtatcgc gaaacttttg tccccaagcg aacggaagag gtgtcgctgt catcagagga
     7981 tcaacaaggt ttaaataaag ttcattcgtt aagtgataaa tgaatttaat tgtttatttt
     8041 atgagtaaaa atgatcttga cataatgaaa atgaatctat aagatcatca ggttcgcctc
     8101 aatgattttt tcaagttaaa tttctcaaaa ttaagatgta attggaatta aaatgccaac
     8161 agtaaaccaa ttgataagag tcggccgtaa ggcaattaaa ataaaaagta agagttcggc
     8221 tttaaatcaa tgccctcaga gaaggggtgt gtgccttcgt gtatatacta ccacgcctaa
     8281 gaagccaaac tcggcattaa gaaaggtggc gagggttcgg ttaacgactg ggcttgaggt
     8341 gacggcatat atacctggta tggggcataa tcttcaggag cattctatcg ttttggtcag
     8401 gggtggtagg gtgaaggacc ttcctggtgt cagatatcac atgatccgtg gggcgttgga
     8461 tgccgtcggg gttgctaatc ggaagcaggg gcgatcaaag tatggagcaa agcgtcccaa
     8521 gtagtaggct tcgcgctttc accttcgctt attcaattta aaactcgcta attttttagg
     8581 ggaaaaaatg ccacgtaggc cactcgttct tcgtcaaaag gtagtgcagg atgccaggta
     8641 taaagaccag gttgttggga ggtttttaaa tattctcctt caggacggaa agaagagtac
     8701 ggctgaaaga gtttgttatg gtgcctttga tattgtcagg gataagaccg gagatgagcc
     8761 tcttaaggta ttccatgctg cccttgggaa tgtcaagccg atggttgagg tgaagtcgag
     8821 gcgggttggt ggggcatctt atcaggtgcc agttgaaatc cgtcctgtgc ggcaggtggc
     8881 gttggctttg aggtggatta agcaaagtgc tctggcgaga agtggcaaga gtatgaggga
     8941 aaaattagcg gcagagctca tggatgctgc gaataataat ggttcggcgg taaagaagcg
     9001 ggatgagacg caccggatgg ctgaggcgaa tagagcgttt gcccattacc ggtggtaggt
     9061 ttcattcttt ggtgttttct tgggtagata gtttaattct ctgtttaatc ttaattgact
     9121 gagcccttgg cggcattttt ttgtgtgagg gatttgtgtc tgcagatttt attttaagtc
     9181 gaactcggaa tattgggatt atggcccata ttgatgccgg gaagactacg acaacggagc
     9241 gggtcctcta ttacaccggg gtttctcata aaattggtga ggtccatgag ggtgcggcaa
     9301 cgatggattg gatggagcag gagcgggaga gggggattac gattacctct gctgctacca
     9361 cctgtttctg gaaagaaagt cggattaaca ttattgatac ccctggtcat gtggatttca
     9421 cgattgaggt ggagcggtcg cttcgagtgc tcgatggggc tatagctgtt tttgattcgg
     9481 ttcagggtgt ggagccgcaa tccgagaccg tgtggcggca ggcagataaa tatcgcgtcc
     9541 cacgaattgt gtttatgaat aagatggatc gagtgggggc tgattttttt gcaagcgtaa
     9601 agtctttggt tgaccggctt ggggccaagc ctgttcctgt gcaattgcct ttgggaagtg
     9661 aggatggatt gcggggggta attgaccttc tggttatgaa ggcgttggtt tttgatgatg
     9721 agactcttgg ggctgcctat tccgttcaag aaattcctga tgaatttatg ggggtggcga
     9781 aagagtaccg cgagaaatta ttggatgccg ttgtagagtt tgatgaagtg gtgatggagc
     9841 ggtatttggc tggagaggtg cttggggtag atgaagttaa gagggtgatc cgggctggga
     9901 cgatagggtt gaaaattaat ccggtttttt gtgggtcggc atttaaaaat aaaggtgttc
     9961 agccgttact cgatggtgtg gtggattatc ttccttcgcc ggttgatctt cctccggtgg
    10021 tggggattga tcctcatact gaaggggaaa tcactcggaa acctcaagat agtgagccat
    10081 ttgctgcgtt ggcatttaag ataatgtctg atcctttttc cggtcaattg acgtatttca
    10141 gggtatattc agggaaatta accgctggat cttatgttta taacgtcgca aaggggaaaa
    10201 aggaacgagt agggcgattg ttgaaaatgc atgctaataa gcgagaagag attgaggaag
    10261 tctctgctgg tgatattgct gcagctgtgg gactcaagga tactcgaacg ggtgatacgc
    10321 tttgtgatga gaagcatcag atattgctgg aagttataaa atttcctgag ccggttattt
    10381 cattggcggt tgagccaaaa accaagcaag atatggataa attgggctat tctttggaaa
    10441 agcttgctca ggaagatcct tcttttcaag ttaaatctga tgatgagacg ggccaaacaa
    10501 tcatttctgg aatgggcgaa ttgcatcttg agatcattgt ggatcggctc cttcgggaat
    10561 ttaaggtgca ggcgaatgta ggaaagcctc aggtagctta tcgcgaaact attaagggaa
    10621 gagctgaggc tgaggggaaa tatgtaaagc agaccggtgg tcgtggtcag tatggccatg
    10681 tgtggttgag ggttgagccc gctgagtctg gcgtagggtt ggagttcgtc aataaaattg
    10741 ttggaggtac ggttccaagg gaatatatcg ctccggttga aaaaggggtt cgtgagcgta
    10801 tggagagtgg aattcttgcc gggtaccctc ttcgtgacct taaggtaact ttatttgacg
    10861 ggtcttttca tgaggtggat tcgagtgaga tggcttttaa gattgcgggg tcgatggcgt
    10921 tggtgagtgc ttgccagaag gccgatttgg ttttattgga accggtcatg aaggttgagg
    10981 tgcttgttcc tcaagatttt atgggggatg tgataggtga tttaaatagt cggcggggaa
    11041 aaattcatgg gatgcgggca agagggacct ctcaaattgt cgatgctgag gttcctttga
    11101 gtgagatgtt tggttattcc acagatttac gttcaaagac tcaggggcgt gcgacgtata
    11161 gtatggaatt tgagtcttat gaagtggtgc caaagctcat ggctcaacaa atcataaaaa
    11221 agaatcaagg ggagtagtat cctcgtaaat tttctaactc ggtacggtaa ggatttcaag
    11281 aggaggtagg gcatggcgaa ggcgaagttt gagcggaaga agccgcatgt caatgtg
//
LOCUS       DAORAQ010000079        11177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_953_length_11177_cov_77.936972, whole genome shotgun sequence.
ACCESSION   DAORAQ010000079 DAORAQ010000000
VERSION     DAORAQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_953_length_11177_cov_77.936972"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..285
                     /locus_tag="PKM72_08475"
     CDS             7..285
                     /locus_tag="PKM72_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNP60859.1"
                     /translation="MDLQGITEGGLRLGVKGGGCSGLSYTVNFDDKIGEFDTVTEVDG
                     VKVIVDAKSAIYLQGMTLDYQKDMVSGAFKFINPNATKTCGCGESFSA"
     gene            343..1092
                     /gene="hscB"
                     /locus_tag="PKM72_08480"
     CDS             343..1092
                     /gene="hscB"
                     /locus_tag="PKM72_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNP60860.1"
                     /translation="MPESSEPEIMEPQQQATTKPRELPMARSMCWHCQSEVTGEYLCG
                     QCVKVQPLSKDLDYFSCFQLPRLLNIDEQQLEQTFYELSRTFHPDFYSTKDESEKAIS
                     LGNSAFLNTGYRILKDPIQRAEYLIRLEAGAVKDIRSNPPADLFEEVLELQEDLETFR
                     QLAPNGTSSELEVLRTKLQDEREGLEKRQAEMEKSLQHKFTEWDQLQGEPPDSSQTRE
                     KKNTLLRGMQEILSNRTYVRNMVDDLLETTG"
     gene            1119..2936
                     /gene="dnaK"
                     /locus_tag="PKM72_08485"
     CDS             1119..2936
                     /gene="dnaK"
                     /locus_tag="PKM72_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNP60861.1"
                     /translation="MGRIVGIDLGTTNSLVAFMDQEGPHVIPGPDGQTKVPSIVGLTD
                     NGLIVGEPAKAHLIRDPSRTIYSVKRFMGKGLDDVKDELKYFSYQLHEKNGVIRIEVG
                     DKTYSPPQVSAMILKELKRRAEEYLKEDISKAVITVPAYFNDSQRQATKDAGLIAGME
                     VLRIINEPTAASLAYGLQKKTQGLIAIYDLGGGTFDISILKLKDGIFEVLATNGDTHL
                     GGDDFDQRLVDVMVDEILRIQGLDIGESLDLMQTVRLEAERVKVRLSDETHTKAVIEL
                     PDGKYEKTWTREDMEALTMDLVERTLAPCRSALKDAGLHANQIDEVVLVGGNTRMPLV
                     WRKVEELFDKKPHSELNPDEVVALGAAVQADILGGHTTDLLLLDVTPLSLGIETMGGV
                     MSALIRRNTTIPTSAKEMFTTYVDGQTSVDIHILQGERELVKDNRSLARFQLKLPPLP
                     AGVPRVEVNFLIDANGILHVTARDIRTGEAQSVQVKPSHGLTDDEVEGMIRDSFQFAT
                     EDIKARQIIEARNEANAIIVATEKALVRATNLINDKETESIRRILQQLGEVKEGEDHR
                     VIRAKIAEAEKTTHHLAEVLMDATLKEALESKKLSDIIK"
     gene            3009..3212
                     /gene="iscX"
                     /locus_tag="PKM72_08490"
     CDS             3009..3212
                     /gene="iscX"
                     /locus_tag="PKM72_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNP60862.1"
                     /translation="MWGDTEDIAIRLQEEHPDLDPLTVRFTDLHAWVVALPEFKDDPK
                     TSNEKKLEAIQMAWYEEFQDAQS"
     gene            complement(3254..5653)
                     /locus_tag="PKM72_08495"
     CDS             complement(3254..5653)
                     /locus_tag="PKM72_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNP60863.1"
                     /translation="MSKSRTFFSQLRGWSWLRITIVTTFLALCIGIGGIAGILWIATR
                     DLPTFDSFQDYHPSLVSRVYAENGEIIGQFFIERRLYTPIDKIPQAFTQAVIATEDTR
                     FFEHPGLDIIGIGRAAWTNMKKGGRFQGASTITQQLARALFLSPERTYQRKIKELILA
                     VKMEWVLTKEQILEMYLNQIYFGHGAYGVAAAALTYFDKNVSDLTLPESAFLAGLPKA
                     PNTYSPYRNPDLAKSRKELVLGRMVEAGYITNEDAQAAMATTLSYRHQSIEPIAAYFL
                     EEVRQHLVDRYGETLVYRGGLRIYTTLNIAMQKTAEEAVRTGLRQLDKRQGWRGPIEH
                     IAFSKDFTPPDTFPELQSPKAALVHGLYRALVTDVTKKSAQVLIGNTYKGSILFEDMR
                     WAQRRLEKNGDVGTAVVRDKASPLQLLKVGDIIEVAPKNGTVESGEFILEQTPIVEGA
                     LIALDPRTGAVQAMVGGYDFTRSQFNRAVVARRQPGSAFKPLIYASALQQGLTPATLI
                     LDAPVVYEDEDLDRIWKPENYEKRFFGTITLREALRHSRNAATVRLLEQIGVPEVVNI
                     ASNLGIRSPLSQDLSLALGSSSVTLQEITSAYGVFADQGLWLEPYLITHAKNLNGEIL
                     EQHLFEPQQAMTKENAYLITNMLMDVIQSGTGRLAKSIGRPLAGKTGTTNSYNDAWFV
                     GYAPNLATGVWVGFDGVRTLGRLESGAHAALPIWTRFMDQALLNSPVMTFPIPSDIQF
                     AQIDTTTGDLPSKNSRNISTEVFRKGTEPGKAAPQKANPMDFFEFDRLNSESTNQLSP
                     F"
     gene            6047..6520
                     /locus_tag="PKM72_08500"
     CDS             6047..6520
                     /locus_tag="PKM72_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNP60864.1"
                     /translation="MSTDVAAEVKVGDVAPDFTLKDQDQKDIKLSDYRGKKNVVLAFY
                     PLDWSPVCTGENKCLTDDFPNFGSVNAEIFGVSCDSFFSHKAWADSLDLKHQLLADMH
                     RTVSKAYGLYFEPLNCSKRATVIVDKNGKVAYAKVQDIKTARDDKEILEALKKLN"
     gene            6554..6937
                     /locus_tag="PKM72_08505"
     CDS             6554..6937
                     /locus_tag="PKM72_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNP60865.1"
                     /translation="MNLLLRSEIFDREAVMSGIVEDVSDDNYEGFTEASAAIVAYGIA
                     TCEPCKAYDPVLAEIAQQFTNVRFGKAKMHVPGRCRAIKKRHQFETYPTTHFFSNGML
                     VLTKEGKLEPAELASLIRQSFSIQT"
     gene            complement(7072..7866)
                     /locus_tag="PKM72_08510"
     CDS             complement(7072..7866)
                     /locus_tag="PKM72_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNP60866.1"
                     /translation="MLKATLTKRLEFCSSHRYHNPQWDDAKNRAVFGLCNNVNTHGHN
                     YLLEVTLRGDIDPVTGMIINLYDLKNILNAVLEQFDHKNLNLDTPYFSERIPTTENFA
                     IILWHILEKHPDLPNPDALRLYEDETLYAEVNASFMDGDLQSSQRDSALIARRYAFSA
                     VHQLRTSNPQGHDYDLWIAIKGLISPDTGQVMNLDTVDQIVRTHVITRFDQRNLSQDQ
                     AFANIPVTDSALAKVIWETLVPHFEAPPLYRVSVSQQPGAVAVYSV"
     gene            complement(7962..8282)
                     /gene="erpA"
                     /locus_tag="PKM72_08515"
     CDS             complement(7962..8282)
                     /gene="erpA"
                     /locus_tag="PKM72_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNP60867.1"
                     /translation="MVTITEIAEQKIKELIKEEENSVGLRIYVKGGGCSGYQYGMSFE
                     DKTSDDDTIIEKGDVKVIVDSQSAPMLSGAEVDYVDSLQGSGFAIKNPQAKSTCGCGS
                     SFST"
     gene            complement(8450..9031)
                     /locus_tag="PKM72_08520"
     CDS             complement(8450..9031)
                     /locus_tag="PKM72_08520"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNP60868.1"
                     /translation="MNLLTPQDLLSAAQYEENRADIRQRIITLKKRRRISVGELVTLV
                     FENRETLLFQIQEMIRIERIFDPGKIQEECDVYNALLPSRDELSATLFIEITDSEKIQ
                     SLLDSFQNIDQADTVGIKVGETSVFANFEAGHSKEDKISAVHFVRFSTTQTFRDLLAQ
                     DEVPAFLTILHPQYRTEAPVPQELRQEWLKDLE"
     gene            complement(9028..10368)
                     /locus_tag="PKM72_08525"
     CDS             complement(9028..10368)
                     /locus_tag="PKM72_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNP60869.1"
                     /translation="MADEKGCRPAITLPKPFQATSQPVPKFDLITPGWTRSQLETETR
                     RIFDVCDGCRRCFNLCPSFNTLIDRIDEYESDSTKFTPQDFTKVEQECYYCKLCFNHC
                     PYSPPHQYDLDFPRLMAAWKKQRTAEGGATWRDKLLIQTDLIGKLGSLTAPLTNWALR
                     TPWLRSLAERTVGVHKNRQVLPFQSQTFTQWWNQRKPAPASRSQGKVAFFPSCLVTYQ
                     VTDIGKATVQILEKNGIEVVVPPNQQCCGMPRFDLGDTDGMAKIAESQYRLFAPYLDQ
                     GYDIVVPAPSCSLMFKREYPYLKPTPEMKQLSERTFDLCEYLMRLKREGKLSLDFQFN
                     PGRVAYQIPCHLRDQNIGFKSKELLELTGATVHLIEKCSGHDGAWSAKTEFFDLSMKI
                     AKKAVREIQEEPFDVVASDCPLSALQLDQALQSAPTQSTLHPIQVVRNAYGLTP"
     gene            complement(10395..10823)
                     /locus_tag="PKM72_08530"
     CDS             complement(10395..10823)
                     /locus_tag="PKM72_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNP60870.1"
                     /translation="MGKALKGTKSHENLKAAFAGESQANRRYLYFARRADIEGYTDIG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKASIEGETYEYTQMYPGM
                     AKTARDEGLEELAEWFETLAKAERSHANRFTKGLETLKQG"
     gene            complement(10807..>11177)
                     /locus_tag="PKM72_08535"
     CDS             complement(10807..>11177)
                     /locus_tag="PKM72_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNP60871.1"
                     /translation="VAPQFPMMSLNTVYYTLGVLRTSGLIHEVNIGHTRARFDANLSP
                     HHHLICVECQAIVDVMDPRLNRLTSPAGIPKDFEITSYQVAFRGYCGSCRRHRGRSIN
                     HSSSGLHPTTVKGGLHGKSS"
BASE COUNT         2921 a         2454 c         2865 g         2937 t
ORIGIN      
        1 cggttattgg accttcaggg aattactgaa ggaggtcttc ggttgggagt gaagggtggc
       61 ggttgttcag gattgagcta taccgtcaac ttcgatgaca agatcgggga gtttgatacc
      121 gttaccgagg tggatggcgt gaaagtgatc gtcgatgcaa aaagcgctat ttatcttcaa
      181 ggcatgaccc tggattatca aaaggacatg gtgagtggag cctttaaatt tattaatccc
      241 aatgccacca aaacctgtgg gtgcggagag tcgttttcag cgtaagatca atttcattca
      301 accacatagt gacgtaacca ctcgggcatg gggctgttcc ccgtgcccga gtcgtcagag
      361 ccggaaatca tggaacccca acagcaagcc acgacaaaac cccgcgaact tccgatggcg
      421 cgcagtatgt gctggcactg ccaatccgaa gtcacgggag aatacctctg tggccaatgt
      481 gtcaaagttc agccactttc gaaagactta gactattttt cctgctttca actgcctcgt
      541 ttgttgaata ttgatgaaca acagttggag cagacttttt atgaattaag tcgtactttt
      601 catccggatt tttattcgac caaagatgaa tcggaaaaag ccattagttt gggaaattcg
      661 gcgtttttga ataccggtta tcggatactc aaagatccta ttcaacgggc cgaatattta
      721 atccgcttag aggctggagc agtaaaagat attcgatcga atccccctgc tgatctgttt
      781 gaagaagttc ttgagttaca agaggatttg gaaaccttcc gccaattggc ccccaatggc
      841 acgtcatcgg aacttgaggt gcttcggaca aagttgcagg acgaacggga aggtctggaa
      901 aaacgacaag ccgaaatgga aaaatccctg cagcataagt tcacggaatg ggatcagttg
      961 cagggagaac cccctgattc gagccaaacc cgtgaaaaga aaaatactct gttgcggggt
     1021 atgcaggaaa ttctctctaa tcgaacatac gtgcggaata tggttgacga tttgctggaa
     1081 acgacgggct aaccgtcatc attaaaaagg aatatgttgt gggtcgtatt gttggcattg
     1141 atttaggaac gacaaactcg ctggttgcct ttatggatca agaagggcct catgtcattc
     1201 caggtccgga cgggcagacc aaagtcccat ccatcgtggg gttaacggat aatgggttga
     1261 tcgtggggga acccgcaaaa gcccatttga ttcgtgaccc ttcccgtacc atttattccg
     1321 tgaagcggtt tatgggaaag gggttagacg atgtgaagga cgagttgaag tatttttcct
     1381 atcagcttca tgaaaaaaac ggagtgattc gcattgaagt tggagataag acctattctc
     1441 cgcctcaagt gtctgccatg attttgaagg aattaaagcg gcgggccgag gaatatttaa
     1501 aagaagacat ctccaaggcc gtgatcacgg tcccggcgta tttcaatgat agtcaacgac
     1561 aggccaccaa agatgccggg ctgattgccg ggatggaagt tttgcgaatc attaatgagc
     1621 cgacagccgc ttcgctggcc tatggcctac agaaaaaaac acaagggctg attgcgatct
     1681 atgatttagg cgggggaacg ttcgatatct ctatcctcaa attaaaggac gggatctttg
     1741 aagtcctggc cacgaatggg gatactcatt tagggggaga tgatttcgat caacggctcg
     1801 ttgatgtcat ggttgatgaa attttaagaa ttcagggctt ggatattggc gagtctctcg
     1861 atttgatgca aaccgttcgg cttgaggcgg aacgcgtcaa agttcggctg tctgatgaaa
     1921 cgcacacgaa ggctgtcatt gagcttcccg atgggaaata tgaaaagacg tggacacggg
     1981 aggacatgga agcgttaacc atggatttgg tggaacgtac tcttgctccc tgtcgaagcg
     2041 cgctcaaaga cgctggatta catgctaatc aaattgatga agtggtcctg gtcggcggga
     2101 atactcgcat gccgctggtc tggcgaaaag tggaagaact ttttgacaaa aagcctcact
     2161 ctgaacttaa tcctgatgag gtcgtcgcgt tgggggcggc tgtgcaagcg gatattttag
     2221 gggggcatac cacggatctg ttgttactgg acgttactcc cttgtcattg ggaatcgaaa
     2281 caatgggtgg ggtcatgagt gcgctgatcc gtcgaaatac cacgattcca accagtgcaa
     2341 aggaaatgtt taccacctat gtcgatgggc aaacctctgt ggatattcat atcctccagg
     2401 gagaacggga attggtcaaa gataatcgaa gtttggcccg tttccaatta aagcttccac
     2461 ctttgccggc aggggtgcct cgtgtggaag tcaattttct cattgatgcc aatgggatcc
     2521 tacacgtgac ggccagggat atacggacag gtgaggctca gtcggttcag gtgaagcctt
     2581 cccatggact gaccgacgac gaggtggaag gcatgattcg ggattctttc caatttgcca
     2641 cagaagacat caaagcccgt cagattattg aagcccgaaa tgaagccaat gcgatcattg
     2701 tggcaaccga aaaggctctg gtgcgcgcaa cgaatttaat taatgacaag gaaactgaaa
     2761 gcatccgccg gattttgcag cagcttggtg aagtcaaaga gggagaggat catcgtgtga
     2821 ttcgtgcgaa aattgctgag gctgaaaaga cgactcacca tttggccgaa gtgttgatgg
     2881 atgccacgtt gaaagaagct ctcgaaagta agaaattatc tgatatcatt aaataatccc
     2941 tcaattggga gactgtccgc agggaaaaat tcattaatcc aaggggaaaa cgtatggctc
     3001 aggaattcat gtggggtgat accgaagata ttgctattcg gctgcaggaa gaacatcctg
     3061 accttgatcc gttaaccgtc aggtttacgg atttgcatgc gtgggtggtt gctcttcctg
     3121 aattcaaaga cgatcctaaa acgtcaaatg aaaaaaaact ggaggccatc caaatggcct
     3181 ggtatgaaga gtttcaggac gcccagtcct gagattgcga aatgctttgc attcgtcctg
     3241 ccgaagagtt caattaaaat ggggaaagct ggttggtgga ctccgagttc aaccggtcga
     3301 actcaaaaaa atccataggg ttcgctttct gaggagccgc ttttcctggt tccgtccctt
     3361 tacgaaaaac ttccgtactg atgtttcgac tgttctttga tggcaaatca ccagtcgtgg
     3421 tgtcgatctg tgcgaattga atatcactag ggatgggaaa ggtcataacg ggtgagttga
     3481 gaagtgcttg atccataaac cgggtccaaa ttgggagggc cgcatgtgct ccggattcca
     3541 atctgcccag tgtccgaacc ccatcaaaac ccacccatac tcccgtcgcc aaattcggag
     3601 catacccgac aaaccacgca tcgttataac tattggtggt tcccgttttt cctgccaatg
     3661 gccgcccaat agatttggcg agtcttcctg ttccgctttg aatgacatcc atgagcatat
     3721 tggtgatcaa gtaggcgttt tccttggtca tcgcctgttg aggttcgaac agatgctgtt
     3781 ctaagatctc cccgttgagg ttctttgcat gcgtaatcag gtaaggttca agccacaatc
     3841 cctgatccgc gaagacgcca taggcagagg tgatttcttg gagggtcacg ctggaagatc
     3901 ctaaggccag ggagaggtcc tggctgagtg gacttcgaat tccgaggttg ctggcgatat
     3961 tgacgacttc gggaactcca atttgttcaa gcaatctcac ggttgcggca ttacgggaat
     4021 ggcgcaacgc ttcacgtagg gtaattgtcc caaaaaatcg tttttcataa ttttctggtt
     4081 tccaaatccg gtctaaatcc tcgtcctcat acaccacggg ggcatcaaga attagggttg
     4141 ccggtgtcag gccctgttgg agtgccgaag cataaatgag tggcttgaat gctgagccgg
     4201 gttgtcgacg tgcaaccact gcacgattaa actgactccg agtaaaatca tatcctccca
     4261 ccatggcttg aacagcacca gtcctgggat ccaaggcgat aagggcgcct tccacaattg
     4321 gagtttgctc taagataaat tccccagact cgacagtgcc gtttttggga gcgacttcaa
     4381 taatatcccc caccttcaga agttggagtg gtgaggcttt atcacggacg accgccgttc
     4441 caacgtcccc atttttctcc aatcgccgtt gagcccagcg catatcctcg aaaagaatag
     4501 agcctttata ggtatttcca atcaagactt gagcagattt tttagtcacg tcggtcacga
     4561 gggctcggta gaggccatgg acaagagctg cctttggact ctgtagttcc ggaaaggtat
     4621 ccggaggcgt aaaatctttg gaaaaggcaa tatgttcaat tggtccgcgc cagccttgcc
     4681 gtttatctaa ttgacggaga cctgtgcgaa cggcctcctc cgcggttttt tgcatggcaa
     4741 tatttaaagt ggtgtagatt cgtagcccac ccctatacac gagggtttct ccgtaccgat
     4801 ccaccaggtg ctgacggacc tcttccagga aataagcggc gatcggttca atggactgat
     4861 ggcggtagga tagggttgtg gccatggccg cttgcgcatc ctcgttggtg atataccctg
     4921 cttctaccat acgacccaaa accaattctt tccgcgattt ggccagatct ggattccgat
     4981 agggagaata ggtgttcggg gctttaggaa ggccagccaa aaaggctgat tcaggaagag
     5041 ttagatccga gacattttta tcaaaatagg ttaaggcggc tgctgccact ccataggcac
     5101 cgtgaccaaa ataaatttgg tttaggtaca tttctaagat ttgctctttg gtcagcaccc
     5161 attccatttt taccgcaaga atgagctctt tgatttttcg ctgatacgtt cgttccggag
     5221 aaagaaaaag ggctcgggct aattgttggg taatggtgct cgccccttgg aaacgaccac
     5281 cttttttcat attggtccaa gccgcccgcc cgatgccgat aatgtctagt ccagggtgct
     5341 caaaaaagcg agtatcctcc gttgcaatca cggcttgtgt gaatgcttga gggattttat
     5401 cgataggggt atacagacga cgttcgatga aaaattgccc aatgatttca ccgttttctg
     5461 catagactct tgatactaaa ctgggatggt agtcttggaa ggagtcaaac gtaggaagat
     5521 cacgagtcgc aatccagaga attcccgcaa tgcctcctat cccgatacac aaggctagaa
     5581 acgtggtgac tatggtaatt cgaagccagg accagccgcg tagctgggaa aagaacgtcc
     5641 gggatttact catagagaat aagtaaccaa aaacggccta gagataggaa agattttgga
     5701 tggtatcaat gaggaagaaa atttcactgg ccttttattt acgatacccc gagatagact
     5761 aaaacacaag atcaatatct gtttcctaca gacggcaatt ggcggccatg gcgtatacaa
     5821 acgatgttgg aattggaatt gtgaaaaata gggcggaagg aagatagcag aattcaatgc
     5881 tcactgccct cttattcgac aaactggctt ggagtggaat gtaggtttgg gactaaatgg
     5941 acaatcctgt aggtcttctg ccagattgac aaactttttc accccatggt accacaaaag
     6001 gtcaagtgaa ttttcggcat ttctattaca acgaaggaga aaaattatgt ctacagatgt
     6061 tgcagcagaa gttaaagtgg gagacgtggc cccggatttt acattaaaag accaagatca
     6121 aaaggatatt aaactcagcg actatcgtgg aaagaagaac gtggttctgg cattttatcc
     6181 tctggattgg agccctgtct gtacaggaga aaacaaatgt ttaaccgatg attttccaaa
     6241 cttcggatcg gtcaatgccg aaatttttgg tgtaagctgt gatagctttt tttcccacaa
     6301 agcttgggct gattccttag atctcaagca tcaattactc gccgatatgc atcggacggt
     6361 gtctaaggcg tatggattat attttgagcc gttgaattgc tcaaagcggg ctacggtgat
     6421 tgtggataaa aatggaaaag tggcctatgc gaaggttcag gatatcaaaa ccgctcgtga
     6481 cgacaaagaa attttggaag cgttgaaaaa gttaaattaa tcctgaggat gttcaaaccg
     6541 aaggagttgg ttcttgaacc tactccttcg gtctgaaatc tttgatcgcg aggctgtgat
     6601 gtccggtatt gttgaagatg taagcgacga caattacgaa gggtttaccg aggcttcggc
     6661 tgcgattgtg gcttatggca ttgcgacttg tgaaccttgt aaggcgtatg atcctgttct
     6721 ggctgaaatc gcccaacagt ttacgaatgt gcgatttgga aaagccaaaa tgcatgttcc
     6781 gggacgatgc cgcgccatta aaaagcggca tcaatttgaa acgtatccca ccacccattt
     6841 cttttccaat ggaatgcttg tgctcaccaa agagggcaaa ctagaacccg ccgaacttgc
     6901 cagcttgatt cgacaatctt tctcaattca aacctagatt ctgttttggt ggatctatat
     6961 tgatccatat tccacacctg gctggcagag gtttcatttt gaccagcaga atataccgat
     7021 tttccccagc gacagatctt tgaatgtgtt gaagggtttt gagattcaga gctatacaga
     7081 ataaacagcc acagcgcctg gttgctggct cacggagact cgatagagag gtggtgcttc
     7141 gaagtggggc accagtgttt cccagatgac ttttgcaagc gcggaatctg taacggggat
     7201 gttggcaaag gcctgatcct ggctcagatt tcgttggtca aaccttgtaa tgacatgggt
     7261 tctgacgatt tgatccacag tgtctaaatt cataacctgc cctgtgtcgg gggaaattag
     7321 gcctttgatg gcaatccaca gatcataatc atgcccttgg ggattactcg tcctgagttg
     7381 atgcactgca gaaaatgcat agcgtcgagc gattaaggcc gaatctcttt gggaggattg
     7441 gagatctcca tccatgaagc tcgcgttcac ttcagcgtag agggtttcgt cttcgtaaag
     7501 acgaagtgca tctggattag gaaggtctgg atgcttttct aaaatgtgcc ataggataat
     7561 tgcaaaattt tcggttgtgg gaattcgttc agaaaaatag ggagtatcaa gatttaagtt
     7621 tttatgatca aattgttcca gaaccgcatt taaaatgttt tttaaatcat ataaattgat
     7681 gatcattcct gtgacaggat cgatgtcccc acgcaaggtg acttccaata aataattatg
     7741 gccatgggta ttcacattat tacagagacc aaaaacggca cggttttttg cgtcgtccca
     7801 ttgaggattg tggtagcggt gggaggagca aaactctagg cgtttggtga gggtcgcttt
     7861 aagcatgggg aacctctata aaaaagaaaa gggctggagc ttggttgcct atgggagtcc
     7921 aagtccagcc ctaattggtt aggggtctga gagaatattt tttaggtcga gaacgagctt
     7981 ccacatccac aggtggactt cgcttgagga tttttaatgg caaatcccga cccttgcaga
     8041 ctatccacgt aatcgacttc tgcgccgctg agcattgggg cgctctggga atccacaatg
     8101 accttcacgt cgcctttttc aatgatggtg tcatcgtcgc tggttttatc ttcaaacgac
     8161 attccatatt gatagccgct gcagccgccg cctttgacgt aaatgcgcag gccgacggaa
     8221 ttttcttctt ctttaatcag ttccttaatt ttctgctctg caatctcagt aatcgttacc
     8281 atagttgttg tatcctcctt ttaatcacac attttttgta gtttcatctt tcctttcaat
     8341 cggttgaaag gagttcaaca ttgagcttac atcttaaagc acgactttta ttcttgtcaa
     8401 tttatagaag gactgagagg ttgcggaatc atgaagagta aatgatgcat cactcaagat
     8461 cttttagcca ttcctgcctc aactcctgag gaactggggc ttctgtgcga tattgagggt
     8521 ggaggatagt cagaaaggca gggacttcat cctgggccaa gaggtctcga aaggtttggg
     8581 tggtggaaaa tcgtacaaaa tgcacggcac tgattttatc ttccttgcta tgcccagcct
     8641 caaaatttgc aaaaactgag gtttctccaa ccttgatgcc cacggtatcg gcctggtcaa
     8701 tattctgaaa agaatccaga agcgattgaa ttttttcgga atcggtgatt tcgataaaca
     8761 gtgtggcgct caattcatca cgacttggta gcaaagcatt gtacacgtca cattcttcct
     8821 gaatttttcc gggatcaaag attcgctcta ttcgaatcat ttcttgtatt tgaaatagca
     8881 gggtttcacg attctcaaac acgagtgtca cgagttcccc tacagaaata cggcgccgtt
     8941 ttttaagcgt aataattcgt tgccgaatgt cggcacgatt ttcttcatat tgagcggctg
     9001 agaggaggtc ttgtggtgtg agcaggttca tggtgttaat ccataggcgt tacgaacgac
     9061 ttgaatagga tgaagggtcg attgcgttgg tgcagattga agcgcctggt ccagttgtag
     9121 tgccgacaga gggcaatcgg aggcgacgac gtcgaaaggc tcctcttgaa tttctcgcac
     9181 agcttttttg gcgattttca ttgagagatc aaaaaattca gtcttggcac tccaggctcc
     9241 atcatggccg gaacattttt cgatcagatg aaccgtggcc ccggtcagct ccaacaattc
     9301 ttttgatttg aagccgatat tctgatcacg taaatgacag ggaatttgat aggccacgcg
     9361 tccaggattg aactggaaat caagggaaag cttgccttcc cgtttgagcc gcattaaata
     9421 ttcacaaaga tcaaacgtgc gctcactcaa ttgcttcatt tcaggtgtgg gtttgaggta
     9481 agggtattcc cgtttgaaca tcaggctaca actgggagct ggcacgacga tgtcatatcc
     9541 ctgatcgaga tagggcgcaa agagtcgata ttgagactcg gcaatcttgg ccattccatc
     9601 ggtatcaccc aagtcaaacc gtggcatccc acagcactgc tggttcggag gcaccacgac
     9661 ctcaatgcca tttttctcca aaatttgcac ggtggccttc ccgatatcag tcacctgata
     9721 cgtcaccagg caacttggga agaaggccac ttttccctgg ctgcgggatg cgggagccgg
     9781 cttccgctgg ttccaccatt gggtgaaggt ctgggattga aacggaagga cctgacggtt
     9841 tttgtggacg ccaaccgtac gctcagccag actacgaagc caaggggtgc gaagggccca
     9901 attggttagc ggagcggtaa ggctgcccaa ctttccgatt aaatcggttt ggatcaggag
     9961 tttgtcacgc catgtggcgc caccttcagc tgtccgctgc tttttccagg cagccatgag
    10021 ccgggggaaa tccaaatcat attgatgggg cggagaataa ggacaatggt tgaaacagag
    10081 tttgcagtaa tagcattcct gttcaacctt tgtgaaatcc tgtggggtga attttgttga
    10141 gtcgctctca tactcatcaa tccggtcaat aagcgtattg aacgacggac ataagttaaa
    10201 gcatcgtcga catccgtcac agacatcgaa gatccgtcgg gtctcggttt cgagctgaga
    10261 ccgcgtccag ccgggcgtga taaggtcaaa ttttggcaca ggttgtgacg tcgcttgaaa
    10321 aggctttgga agagtgattg cagggcggca acctttttca tcagccaccg ccatgcgtca
    10381 taatgccgaa attatcagcc ctgtttcaga gtttccagcc ctttcgtgaa ccggttcgca
    10441 tgggatcgtt ccgctttagc caatgtttca aaccattcgg caagttcctc aaggccttca
    10501 tcccgagcgg ttttcgccat accgggatac atctgggtgt attcataggt ctcaccttca
    10561 atcgacgctt tcaaattggc ttcggtgttg ccaatcggca cgccggtggc aggatcaccg
    10621 acttctttca aaaagtccaa gtgcccaaac gcatgcccgg tttcagcttc cgaggtatca
    10681 cgaaagagac cgccgatatc ggtatagcct tcaatatccg cgcgtctggc gaaatataaa
    10741 tagcgccgat tcgcttgcga ttcgccggca aacgccgctt ttaagttttc atggctcttc
    10801 gtgcctttaa gagcttttcc catggagtcc tcctttaacg gttgtggggt gtaaccccga
    10861 tgatgaatgg ttgattgagc gcccgcggtg gcggcgacag gagccgcaat atccacggaa
    10921 ggcgacttga taactggtaa tttcaaaatc tttgggaatg ccggccgggg aggtcaggcg
    10981 atttaatcgg ggatccatga catcgacaat ggcttggcac tccacacaaa tcaaatggtg
    11041 atggggagac aggttggcat caaatcgagc ccgggtatgg ccaatattga cttcatggat
    11101 caagccggag gtacgtaaga ccccaagtgt gtagtagacg gtgttcagag acatcatggg
    11161 aaattgcgga gcgactt
//
LOCUS       DAORAQ010000080        11075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_965_length_11075_cov_61.663158, whole genome shotgun sequence.
ACCESSION   DAORAQ010000080 DAORAQ010000000
VERSION     DAORAQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_11075_cov_61.663158"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="PKM72_08540"
     CDS             complement(<1..1747)
                     /locus_tag="PKM72_08540"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNP60872.1"
                     /translation="MTDSTAQEVESFKEIERIEVRSLIHLASEAISQFWPMQTFIHHN
                     PLHGLEHLPIHRAIAEAGTFLGGRGYLPNQEYRDLYRQGRFLEEAITETVAPFAGNHF
                     VTIGTRNISHLEILRMLLIHGTGAVPHDVRNAVFLNACEDPRVRELFLRLQPRGTHGD
                     AHLSFRHQTDQAGKNLGTQETLAAWCDRMCDTSLQEEINQELIKWCAGFLDQGHAPWP
                     MPMREKTFYGSWKALAQREGSGFLLGIPDWTSKLQHLPDRPEDAVLACLQSMGIPHDL
                     WSNVFTLHLAQLPGWTGFLKWRADQVDHEWQAAFPIDLVQYLAVRLFYERELVQRVCQ
                     DRLRLPGTYTAILSSMEHFPFGYGLFRMFRAGRLDPEIFSTLDETLFNTTPLPLQTLD
                     GVGREADAKWGSVQQDLTAKGDTLLLCHLAGSLGVPLNDLVECPPEVLDTLLDWTANF
                     PDSTHGPLWLQAFEMSYVKGFVRGLSPNLAIVHESDSKKDKPTFSRPPAQAMFCIDVR
                     SEGFRRHLEEAGGNETFGFAGFFGLPICFQSFGSEQETDQCPVLLKPKHVVREIPRAS
                     QGKAAERFLERQTLAK"
     gene            complement(1790..2965)
                     /locus_tag="PKM72_08545"
     CDS             complement(1790..2965)
                     /locus_tag="PKM72_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60873.1"
                     /translation="MERTVPSGLWGIGLATILVAGGGLFLFGWEMESDPSGRLLAAAI
                     LLACFCGLLGQQGSSRGSDDVGPILLLGGLGLGGVLGMEPFNRIFVIGLLAYAGFFLA
                     RHMAASLQKSLALVHVSLALMLAVLAIFMEEGRGVVGVLFLVLTFLPLVPFHLPFLGI
                     LRSSRESLGSVWLVVWLAAGLSQLQDLEAMLILHVEWKGVLPVLALVSALYASLKAMG
                     HRLPRAGLAYATITFLALLWGLSGEFSHIAEWGLPFGMAVALLMSALLCAYAFLHERY
                     GAHPLNTLQGLGAGLPRFRGIFTFLISLIMLLPVLPVVAAFKNIPPDAPEAHFLFPVW
                     LLLFTVWLSVSWVFTTMLHQTAFGKPRSDVPHRDLSVYELWTLLLLMVTASMCGMMA"
     gene            complement(3071..4759)
                     /locus_tag="PKM72_08550"
     CDS             complement(3071..4759)
                     /locus_tag="PKM72_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNP60874.1"
                     /translation="MAIFIPLLPLLAAILIGWQGKRLQSHVALIGIVATAGSSLFSFL
                     TLYHVSTQGPIHLTFWSWGQEAAPSLTVGMLVDRLTAVMMVLISSVSTVIHLYSRRYL
                     EGDPGYQRFFGLLGLMTFTILSLVASPNFLVLFLFWQLLSWVLYLLLAFNVSHALASE
                     YARKTLLVHRIGDVSFLCGLLLIYQYFGTFEFAAVFQQAASDPPMIRLWAGSPLELSI
                     VPVIALLIFVGAMAKSAQFPLHVWLPDTMDSPTPVSALMHAGIINAGGFLLNRLAPLY
                     ALSPTTLHLVFIVGAGTVLLGASMMLVQNDIKKTLGFSTMGQMGYMIMECGLGAFALA
                     IFHLIAHGFFKATLFLASGSLIQAARHEPKFPPSSDHRPTKLPTGLTWMTGLSVTLLM
                     PLFILLVAHGVLEVPLQDAHGAVIFLFFGWVTASQAIFSLYRLQAVASWKVAGAMIGT
                     LFFIGFVYLWAGETFTHFLYPDPGVANGFFQAAAFPPQVFDGMILVATLVVLFGWLIM
                     YTTARGQKILNPHWVLSIRQTGWVLFINRCYVDGLFKKLGSALLRLAEKVAYRY"
     gene            5456..6406
                     /locus_tag="PKM72_08555"
     CDS             5456..6406
                     /locus_tag="PKM72_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HNP60875.1"
                     /translation="MPDKKIIAVVGATGAQGGGLVRAIVHDQNSGFIARALTRDIHSD
                     KAKALATLGVEVVATDLDDPESLKRAFEGAYGAFCLTSFWEHGSPEKELAQAKAQAHA
                     AQQAGVSHVIWSTLEDTRQWIPLEDTRMPTLMGKYKVPHFDAKGEANDEFSKRGVPTT
                     FYLTSFYWENLINFGMGPKTGQDGILRITLPMGNKKLPGVAVEDIGKGALGILKKGRE
                     FIGKTVGIAGEHLTGDQMAAALMRAIGQKVQYQSVEPAVYATFGFPGADDLANMFQFK
                     QEFESQFCGARDTDLSRRLNPDLQTFDQWLAQNKNRIPIE"
     gene            6626..6862
                     /locus_tag="PKM72_08560"
     CDS             6626..6862
                     /locus_tag="PKM72_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNP60876.1"
                     /translation="MATMNVSVPDPMKDWVQGQIETGKYANASDYVRNLIRRDQEKIE
                     VLRKTLLEGERSGVSRRKVEDIMNDVKKRRVDNE"
     gene            6855..7145
                     /locus_tag="PKM72_08565"
     CDS             6855..7145
                     /locus_tag="PKM72_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNP60877.1"
                     /translation="MSEYQLSKKADQDLQRTYEFSIQQFGQNVADEYFLSLRDCLMQV
                     ADSPTLGQDCSEIVPELFRFECGPHSIFYKTGKKRIVIVRVIHQSMNPDIHM"
     gene            7235..7936
                     /locus_tag="PKM72_08570"
     CDS             7235..7936
                     /locus_tag="PKM72_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP60878.1"
                     /translation="MKYCLQLHDQQWQLIDQDEPDLTPVVIDFVSGKTAYRRKYGHAG
                     GEAISKAVGIKKGKRPTIVDATAGLGRDAFVLATMGCRVHMIERSDMISKLLEDGLRR
                     AVVDEKIGALIKDKLSLNCGDSRQALLHLPFTPEVIYVDPMFPLKDKSALVKKDMRIV
                     QDIVGHDTDADELLKVALTIATNRVVVKRPAYADYLAGIKPQTSIKTKKHRFDIYLTP
                     RPVEHGHPQRNERSG"
     gene            8211..10886
                     /gene="pepN"
                     /locus_tag="PKM72_08575"
     CDS             8211..10886
                     /gene="pepN"
                     /locus_tag="PKM72_08575"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNP60879.1"
                     /translation="MSEVNSVTYLKDYTQPDYWVTHVDLTFDLRDGETLVSAKLQVKK
                     NGSHDHPLVLDGEELELLEVKLNGLAIPKKANLTEGYIVDQKSLSLQPTGDAFTLETS
                     VKIHPEQNTALEGLYKSGGNWCTQCEAEGFRRITFFPDRSDNMATYTTKIIADQIAAP
                     VLLSNGNLVDHGPMDGQRHYAVWEDPFPKPSYLFALVGGDLACIDDTFVTASGRQVVL
                     CIYADEKDLDKLDHAMASLKRAMTWDEEVYGREYDLDLFNIVAVDDFNMGAMENKSLN
                     IFNTKYVLAHPSTATDADYQGVEGVIAHEYFHNWTGNRVTCRDWFQLCLKEGLTVFRD
                     QEFSGDMGSQAVNRIDNVRSLRMSQFPEDAGPMAHPPRPNQFVTINNFYTATVYSKGA
                     ELNRMLHTLLGKEDFRKASDRYFERFDGQAVTVEDWVHCMEEASGRDLSQFMLWYTQA
                     GTPTVKADWSWDQAKQMFTLTLEQIVPTIQNQSNGQPRHIPVKYGLVGPDGQDVSQGL
                     LELTQAKQTFTFENIPSDCVPSLFRHFSAPVNLEAPYTDDQLRHLMVHDRDGFNQWEA
                     GNRFLTTKLMDQIDRDERGEDILVGEDVLDSFRRILQRTDMDQELMSLALSLPVYQEL
                     APNRTVINPQAILKVRKTFLETIGRELHDKFLEIYHTNYDPGKAYDRADAGRRSLQNT
                     ALAYLSHSGDSDVAKLAVKQYNQANNMTDRYAAMSIIINMDEAQSYRDEVTQHFYNQF
                     ANDALVVDKWMGAFARSQNDNILQIVKDLTGHEAFTHPTPNRMRALYGTFSHGNPKGF
                     HAEDGSGYAFVADFLMELDAKNPQVASRMIGAFEKFRQHRLDLQNKMEAQLRRIAEMK
                     SLSTDLSEKLERYLGKETFSQLRAGKEDAQPPSPA"
BASE COUNT         2971 a         2953 c         2897 g         2254 t
ORIGIN      
        1 tcttggccag agtctgacgt tcgagaaatc gttcagccgc ttttccctga gaggcacggg
       61 gaatttccct gaccacatgc ttgggtttga gcaggaccgg acattgatcg gtttcctgtt
      121 cactcccgaa actctgaaaa cagatgggaa ggccgaaaaa tccggcgaat ccgaacgttt
      181 cattcccccc cgcttcttca agatgccgtc ggaacccctc agatcgcaca tcaatacaaa
      241 acatcgcctg ggccggcggt ctggaaaacg tgggtttgtc cttcttggaa tcactttcat
      301 gcacgatggc caaattggga gaaaggcccc tgacgaagcc tttcacatag ctcatctcaa
      361 aagcctggag ccagaggggc ccatgcgtgg agtcgggaaa attggcggtc caatccagca
      421 gcgtatccag gacttcaggc ggacattcca ccaggtcatt cagcggaact cccagtgatc
      481 cggccagatg gcaaagaagc aacgtgtctc ccttggccgt taaatcctgt tggaccgatc
      541 cccatttggc gtccgcttca cgtcccacgc catcaagcgt ttgcaaggga agcggggttg
      601 tgttgaatag agtctcgtcc aaggtggaga atatctccgg atccagcctt ccggcacgaa
      661 acatccggaa gagaccatag ccaaaaggga aatgctccat ggaggacagg atagccgtat
      721 aggtgcccgg aagtcggagg cgatcctgac acacccgctg caccaactca cgctcgtaaa
      781 agagacgaac cgccagatat tgaaccagat cgatgggaaa ggccgcctgc cattcatggt
      841 caacctggtc ggcacgccac ttcaaaaacc cggtccaccc cggaagctgg gccaggtgca
      901 gggtaaacac attgctccag agatcgtgag gaatccccat ggactggaga caggcgagga
      961 cggcatcttc cggccggtcg ggaaggtgtt gcagtttgct ggtccaatcc gggatgccca
     1021 gcaggaaacc ggagccctcc cgctgagcca aagccttcca actcccataa aacgtctttt
     1081 cccgcatggg catgggccag ggagcgtgcc cttgatccag gaaccccgca caccacttga
     1141 tgagttcctg attgatctct tcctgaaggg acgtgtcaca catccgatcg caccatgcgg
     1201 ccagcgtctc ctgtgtcccc agattctttc cggcctgatc tgtctggtgg cggaaggaca
     1261 ggtgagcgtc cccatgcgtg ccacggggct gaagccggag aaacaattcc ctcacacggg
     1321 ggtcttcaca cgcattgaga aagacggcat tccggacatc atgggggacg gcgccggtcc
     1381 catgaatcag gagcatccgc aagatttcga ggtgggaaat gttccgcgta ccgatcgtga
     1441 caaaatgatt gccggcaaac ggtgcgacgg tttcggtaat cgcctcctcc agaaaccggc
     1501 cctgccgata gaggtcacga tattcctgat tgggtaaata tccccgtcct cccaaaaacg
     1561 ttccggcctc cgcaatcgcc cggtggatgg gtaaatgttc caggccatga agcggattgt
     1621 ggtgaatgaa ggtctgcatg ggccagaact gggaaatcgc ttcactcgct aaatgaatca
     1681 gcgacctgac ctcgatgcgc tcgatttctt taaatgactc gacttcctgt gccgtggaat
     1741 ctgtcatcgt ctccatccct tggtgtgttg catcagacat tcaaccgtac taagccatca
     1801 ttccgcacat gcttgccgtg accatcaaca ggagcaatgt ccagagttca tagaccgata
     1861 aatcccgatg gggaacatcg gatcggggct ttccgaacgc ggtttgatgc agcatcgtgg
     1921 taaagaccca actgactgaa agccacaccg tgaacaacaa cagccaaacc gggaacagaa
     1981 aatgtgcctc aggggcgtcg ggcggaatat tcttgaatgc ggccactacc ggcagcaccg
     2041 gtaacagcat aatcagagaa attaaaaacg tgaagatgcc ccgaaaacgc ggcaacccgg
     2101 cacccaaccc ttgcagagtg ttcagaggat gggccccgta tcgttcgtgt aaaaaggcat
     2161 acgcacacaa cagcgcactc atcaacaatg cgaccgccat cccgaaggga agcccccatt
     2221 ccgctatatg agaaaactca ccactcaacc cccacagtaa cgcgaggaag gtgatcgtgg
     2281 cataggccag gcctgcacga gggaggcgat gtcccatcgc tttcagggag gcatagaggg
     2341 cgcttaccag cgcaagcacc ggcaacacgc ccttccattc cacatggagt atcagcatgg
     2401 cctccaggtc ctgaagctgg gacagccccg cagccagcca gaccacaagc cagacactgc
     2461 cgagagattc gcgggacgac cggaggatac ccaaaaaggg cagatgaaaa ggcacgagag
     2521 gtaaaaaggt gaggaccaga aacagcactc cgacaacgcc gcgtccttcc tccatgaaga
     2581 tcgccagcac agccagcatc agggctaacg aaacatgcac cagagccagg gacttttgaa
     2641 gagaagcagc catgtgtcgc gccagaaaaa agcccgcata ggcgagaagg ccgatgacaa
     2701 agatccggtt gaacggctcc ataccgagca ctccgcccag gcccagtccg cccagcagga
     2761 ggatgggccc cacgtcatcg gaaccacgtg atgagccttg ttgaccaagg aggccacaga
     2821 aacaggcaag aagaatggcg gcggcaagga gacggccgga gggatcggac tccatttccc
     2881 atccgaataa gaacaagcct cccccggcca ccagaatggt ggctaacccg atcccccaca
     2941 accccgatgg cacggttcgc tccatattaa ttccccaccc tcctaaaacc ctgcatacaa
     3001 gagaaggacg gacaccagtc atgttcaagg gccatattca ccagacattc tcatttgacc
     3061 aagacgaaga ctaataccgg tatgccactt tctccgccaa ccgaagcagg gcagaaccca
     3121 gctttttaaa caggccatcc acataacacc ggttgatgaa gagaacccaa ccggtctgcc
     3181 gtatcgagag cacccaatga ggattgagaa ttttctgtcc acgagccgtg gtatacataa
     3241 tcagccagcc aaacagcacc accagcgtcg ccaccaggat catcccatcg aacacctgtg
     3301 gcgggaaggc ggcggcctgg aaaaatccat tggcgacgcc cggatccgga tacagaaaat
     3361 gcgtgaatgt ttcgcccgcc cataaatata cgaatccgat aaaaaacaac gtcccgatca
     3421 tcgcaccggc gaccttccag gaggccacgg cctgcaggcg atagagactg aaaatggcct
     3481 gggaagccgt tacccatccg aaaaagagaa aaatcaccgc cccatgagca tcctgaagtg
     3541 gaacctctaa cacgccatgg gccaccagaa gaatgaacaa cggcatgagc agcgtaacgc
     3601 tcagcccggt catccaggtc agcccggtcg gcagcttggt cggtcgatga tcggaggagg
     3661 ggggaaattt cggttcatgt cgcgcggctt gaatgagact gcccgaagcc agaaacaggg
     3721 tggccttgaa aaacccatgg gcaatgagat ggaagatggc cagggcaaac gctcccaggc
     3781 cgcattccat aatcatatag cccatttgtc ccatcgtgga gaaccccaac gtttttttga
     3841 tgtcattctg aaccaacatc atcgaggcgc cgagcaggac ggtgccggcc cccacgatga
     3901 aaacgagatg aagcgtcgtg ggtgagagtg cgtacaatgg ggccaaccgg ttcagcagga
     3961 accctccggc gttgatgatc ccggcatgca tgagagcgga gaccggcgtc ggggaatcca
     4021 tcgtatccgg aagccagacg tggagaggaa attgcgcgga tttagccatc gcgccaacaa
     4081 aaatcagcag ggcgataacc ggaacaatgc tgagttcaag gggggaaccg gcccacaacc
     4141 gaatcatggg agggtcagaa gccgcttgtt gaaacacggc agcaaattcg aacgtaccaa
     4201 aatattgata aatcagcagg aggccacaaa gaaaactgac atctccaatt cgatgaacca
     4261 ataaggtttt tcgcgcatac tcggaagcca gggcatgaga aacattgaag gccaggagga
     4321 gatacaacac ccaactcaag agttgccaga acagaaataa caccaggaaa ttcgggctgg
     4381 ccaccaatga gaggatggtg aacgtcatta acccgagtaa cccgaaaaac cgctggtacc
     4441 cgggatcacc ctctagatag cggcgggaat acagatgaat gactgtgctg acactggaga
     4501 tgagcaccat catcaccgcc gtcagccgat cgaccaacat ccccacggtc agggaaggag
     4561 cggcttcctg cccccaggac cagaaggtga ggtgaatagg accctgcgta ctgacgtggt
     4621 agagggtcag aaaagagaac aggctggacc cggccgtggc cacgatcccg atcaaggcaa
     4681 catgggactg cagtcgttta ccctgccatc cgatcagtat ggcggcgagc agcggaagga
     4741 ggggaatgaa aatagccatg ttcataggga atcaaccacg ttctggaatg cgaagcagca
     4801 tcgggaacgc cccgatggct cgtacgccgc gacgtgtttt cctgttaata atattccgtc
     4861 ttgctccaat caagcataat ccttttactt aacgattgat attgcaatac gcaataatgt
     4921 gttagaccta attcatgggc ctttcggttc tcacctcttg atatcttttg gaggaaaatt
     4981 accaagtcac cgagcggctc cctccacccc gcagaagagg tgaacagcta tccacggcgc
     5041 gattcttctg aagaagaatg gttcctgtca gctcgtttag cgatgcgcaa ggcgggatag
     5101 tcttccatgt actatatagg cctcttgatc catgaggata tattcaaaaa taaacgataa
     5161 aaagaagaaa tacatacacg gagatcattt tttaaagacg acgggcaaga aaccgccttg
     5221 taacccacgc ttgactttcc ttctgacatg cctccacctc ccctaccagc cgggaaaagt
     5281 tgcgagaaga aacacaccct tctgggactt ttaagatttt ccggacgacg gttccgaaag
     5341 gtataattcc ccacaatcat ttctacgcag gtgaggctct ggcggaattg atcggtttga
     5401 atcggtgaag caaatatcta ttgcgatcgt ctaatctcag aagaagggat ccaatatgcc
     5461 agacaaaaaa attattgccg ttgtgggcgc cacgggtgcg caaggtggcg gtttagtccg
     5521 tgcaattgtg catgaccaga atagtggctt cattgctcgc gcgttgacgc gggacattca
     5581 ttcggacaag gccaaagctc tcgcaaccct gggggtggag gtggtagcga ccgacttaga
     5641 cgatcctgag agcttgaaac gggcttttga gggtgcctac ggcgcattct gtctcacgag
     5701 tttttgggag catggttccc cggagaagga gttggctcag gccaaggcgc aagcgcacgc
     5761 ggcccaacag gccggagtgt cccacgtcat ctggtccacg ctggaagaca ccagacagtg
     5821 gattccgctc gaagataccc gcatgccgac cttgatggga aagtacaagg tcccccactt
     5881 cgacgcgaag ggtgaggcga atgacgagtt ttcgaaacgc ggggtgccga caacctttta
     5941 tctgacctca ttctattggg agaacctgat taactttggc atgggcccca agacgggtca
     6001 ggacggaatc ctccggatca ctttgccgat gggaaacaaa aaacttcccg gtgtcgcagt
     6061 ggaagatatc ggaaagggcg cactgggcat cctgaagaaa ggccgtgaat tcatcggaaa
     6121 aacggtcggg attgccggcg agcatttgac aggcgaccaa atggcggcgg cactcatgcg
     6181 cgccatcgga cagaaggtgc agtatcaatc agtggaaccg gctgtctatg ctacgttcgg
     6241 atttcccggc gcggatgact tggccaacat gtttcagttc aagcaggagt tcgaatcaca
     6301 gttttgcggg gcacgcgata cggatctcag ccgaagactg aatcccgatt tgcaaacctt
     6361 cgaccaatgg ctcgcgcaga acaaaaaccg cattccgatc gagtaaccct gacgcccccc
     6421 tgtgccctgc tgagcccctt catgtcattc tcatccgttc gccttggcac acggtcctta
     6481 cggtccgatc gctcactcat tgtgccatta ctaaagtctc tggcagaaat caatctgtct
     6541 ctcaaatgcg aatcccccca cccatggtgt cactggttgg aacggattgg ctatctatgc
     6601 cagaatgaaa cgtggaggat aagaaatggc aacaatgaat gtgtcggttc ccgatcccat
     6661 gaaggactgg gtgcagggcc aaattgagac gggaaaatat gccaacgcca gcgattacgt
     6721 gcgaaatctg atccggcggg atcaagaaaa aatcgaggtg ttacggaaaa cacttctcga
     6781 aggagagcgt agcggtgtga gccgccggaa ggttgaggac attatgaacg atgtcaaaaa
     6841 acgacgtgta gacaatgagt gagtatcaac tttcgaaaaa ggcagaccag gacctccaga
     6901 gaacctacga attttccatc cagcaattcg ggcaaaacgt ggcggatgaa tatttccttt
     6961 ccctcagaga ttgcctgatg caggtggccg atagtccaac attgggacag gattgcagtg
     7021 aaattgtgcc ggaattgttc cggttcgaat gtggccccca ctcaattttc tataagacgg
     7081 ggaagaagcg aattgtgatc gtcagagtca tacaccaaag tatgaacccg gacatacaca
     7141 tgtaaatcag cgtgatgacg gcgcgactct tcggttaact cccctcccca tcctccctac
     7201 tgaaatcatt gccaaagaaa ttcctagaaa acagatgaag tattgtcttc aattacacga
     7261 tcaacaatgg caattgatcg atcaggatga accggacctg accccggtgg tcattgattt
     7321 tgtgtccggc aaaaccgcgt atcgaagaaa atatggccac gccggtggcg aggccatcag
     7381 taaagcggtc ggcatcaaaa aaggcaaacg accgacgata gtggatgcca ccgccgggtt
     7441 ggggcgtgat gcctttgtgc tggcgacgat gggatgccgc gtgcatatga tcgagcgatc
     7501 ggacatgatc agcaaactat tagaagacgg attacgtcgt gccgtggtag atgagaaaat
     7561 tggcgcgctc atcaaagaca agctatccct taattgtggg gatagccgac aagccttgct
     7621 ccatctccca tttacacccg aggttattta tgttgatccc atgtttcccc tcaaggacaa
     7681 atcggcgctc gtcaaaaaag acatgcgaat tgtccaggat atcgtcgggc atgatacaga
     7741 tgccgatgaa ttactgaagg tagccctcac gattgccacc aaccgtgtgg tcgttaaacg
     7801 cccggcctat gccgattatc ttgccgggat caaaccccag acctcgatca aaacaaaaaa
     7861 acaccgcttc gacatctatc tcactccccg tccggtagaa catggtcatc ctcagagaaa
     7921 cgaaagatcc ggctaagccg tgacaactac agcattcgct caggcctttc gataatttgc
     7981 accaggcctt ccttgtctac ccaacatcag tggaaagaag aacatatgaa gcggtcccac
     8041 cgaacctccc gggccaggca tcaggcttct tactccgtct tactcatttt ggaaccgcga
     8101 gaccgtaaga ccgtgagcct atcctcttgc gtatgcggtg aggtatgtta acaatgaagc
     8161 ctctggccat agccggatat tgaggatgat gaacgatagg agatgatgcc gtgtctgaag
     8221 taaattctgt gacatacctg aaagactata cgcaacctga ttattgggtc acacatgttg
     8281 atctgacgtt tgatttacgc gatggagaga cgttggtcag cgccaagtta caggtgaaga
     8341 aaaatggcag tcatgatcac cccttggtcc ttgatggcga agaactggaa ttgctggagg
     8401 tgaaactcaa tggcctcgcg atccccaaga aggccaatct gacagaaggc tacatcgttg
     8461 atcaaaagtc cctttcactc caacccacag gtgacgcctt tacgctggaa acgagtgtca
     8521 aaattcaccc cgaacagaac accgcccttg aaggcctgta taaatcaggg ggcaactggt
     8581 gcacgcaatg tgaggccgaa gggttccggc gaatcacctt tttccccgac cgctcggata
     8641 atatggcgac gtataccacg aagattattg ccgatcagat tgcggccccg gttctccttt
     8701 ctaacgggaa cctcgtcgat catgggccaa tggatggtca acggcattat gcggtgtggg
     8761 aagatccttt ccccaaaccc agttatttat ttgcccttgt cgggggtgac ctcgcatgca
     8821 tagacgatac cttcgtgact gccagtgggc gtcaagtggt gttgtgtatt tatgcggatg
     8881 aaaaagacct cgacaaactt gatcatgcca tggcctccct gaaacgtgcg atgacatggg
     8941 acgaagaggt gtatggtcgt gaatatgatc ttgacctgtt caatattgtg gcggttgacg
     9001 atttcaatat gggagccatg gaaaacaagt ccctgaatat tttcaacacg aagtatgtgt
     9061 tggcgcatcc ttctacggcg accgatgccg attatcaagg cgtggaaggg gtgattgcgc
     9121 acgaatattt tcacaattgg accgggaacc gggtgacatg ccgggactgg tttcagttgt
     9181 gtttaaaaga aggcttgacg gtctttaggg atcaggaatt ttccggggat atgggttcgc
     9241 aagcggtgaa tcgtatcgat aacgtccgaa gcctacggat gagtcaattc cccgaagatg
     9301 ccgggccgat ggcccatcca ccgcgaccca atcaatttgt caccatcaat aacttttaca
     9361 ccgctaccgt ctacagcaaa ggtgcggagc tcaataggat gttgcatacc ctattgggca
     9421 aggaagattt ccgtaaagcg tcggataggt attttgaacg gttcgacggg caagcggtca
     9481 cggttgagga ttgggtgcat tgtatggaag aggcctccgg tcgggacctc agccagttca
     9541 tgctctggta cacccaggca ggcacaccca cagtgaaagc cgattggtca tgggatcagg
     9601 cgaagcaaat gttcaccctc acgctcgaac agattgtccc aaccatacag aatcaatcaa
     9661 acggtcaacc cagacacatt cccgtgaaat atgggttggt agggcccgat ggccaggatg
     9721 tcagtcaagg attgctggag ctcacgcaag cgaaacagac atttaccttc gagaatatcc
     9781 catctgactg tgtcccttcg ctcttccgcc atttttccgc tccggtcaat ctggaggctc
     9841 cgtatacaga cgaccaattg cgacatctga tggtgcatga ccgtgatgga ttcaatcagt
     9901 gggaagcagg caatcgattc ctcaccacca aactcatgga tcaaattgat cgtgacgaac
     9961 gcggagaaga catcctcgtt ggcgaagatg tcctggattc atttcgtagg attttgcaac
    10021 gaacagatat ggaccaggaa ctcatgagcc tggctctcag cctacctgtc tatcaggaac
    10081 tggctcctaa ccggacggtg attaatccgc aagcgattct caaagtacgc aaaacatttc
    10141 tggaaacaat cggccgtgag cttcacgata agtttctcga gatttaccac accaactatg
    10201 atcccgggaa ggcatatgat cgtgcggatg ccggccgccg cagcctgcaa aacaccgcgc
    10261 tcgcgtattt gtcgcattca ggagactcag acgtggccaa gctggccgtg aagcaatata
    10321 accaggccaa caatatgacc gaccgctatg cggctatgtc gatcatcatc aatatggatg
    10381 aggcgcaatc ctatcgagac gaggtgaccc aacattttta caatcaattt gccaatgatg
    10441 cgttagtcgt cgataaatgg atgggggcat tcgcgagatc acagaacgat aatattctcc
    10501 agatcgtaaa agacctgaca ggacatgagg cctttacaca ccccaccccg aacagaatgc
    10561 gagcgttgta tgggaccttt tcacatggca accccaaagg ttttcatgcg gaagacggat
    10621 cagggtatgc ctttgtcgcg gactttctca tggaactgga tgccaaaaac ccccaagtgg
    10681 cctcaagaat gattggggca ttcgagaagt tccggcaaca tcgattggat ttacaaaaca
    10741 aaatggaagc acaactccgt cgaatagccg aaatgaagag tctttcaacc gatctcagtg
    10801 aaaagctgga gcggtatttg ggtaaggaga catttagcca gctcagggct ggtaaagagg
    10861 atgctcaacc accatctccg gcataaacat gagagcaggc ttgaagacag agagggcgca
    10921 cagttgagcg agtcctatca atcaatacct ccccaatcct gccatttcct aatcgaagcc
    10981 catgctccgc ctccgtcatt cccgacatcc atcatcgggc atccctctaa acttcctcct
    11041 cctcaccttc aggtcggcta cccgggctga cgttt
//
LOCUS       DAORAQ010000081        11073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_966_length_11073_cov_75.065257, whole genome shotgun sequence.
ACCESSION   DAORAQ010000081 DAORAQ010000000
VERSION     DAORAQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_11073_cov_75.065257"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKM72_08580"
     CDS             <1..501
                     /locus_tag="PKM72_08580"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF026049.2"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNP60880.1"
                     /translation="SLLTTLKGLPLSYNRDLQEDKEPLFDTLDTVTTSVKIYAELLAQ
                     LTIRPDSMKEAVSEGFLLATELADYLVKKGMPFRESHHVVGSLVRECLAKGKDLGQLT
                     QQDLVNASSSFDAKAFQVLTPEAAINSKNIIGGTATPQVTKQINGWKKSLQAEMKRSM
                     KKTRTA"
     gene            498..749
                     /locus_tag="PKM72_08585"
     CDS             498..749
                     /locus_tag="PKM72_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60881.1"
                     /translation="MSHIFRTSSQYFLPFCMLVLLFSPIGCGAVGPPIPPEKVGIEAK
                     ILEQQREQARIEGGIDEDPSNVPLEEAVELPTVYPIDTR"
     gene            837..2714
                     /locus_tag="PKM72_08590"
     CDS             837..2714
                     /locus_tag="PKM72_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP60882.1"
                     /translation="MDTPLTHSEIRPKLLLLDPYPRNNPYRMSASERRSIWFPKLSLP
                     AIAAYTPTTWDVEIVDEAVMDINFETPCDVVGISIMTCYAPRAYEIADEFRRRRKPVI
                     LGGVHPTYCPDEALRHCDAIICGEAEDLWPQVITDIEAGALKRIYRMEQFPTLSHYKP
                     PRIELLSPDSYMTRLCTFTTRGCHFDCEFCSVSPFNGKTTRRRPVPEVIEELKRAKEW
                     LRSDIVERMTHGSLFHALTTSLKIWIGLEEGSIVAFVDDLHNSHRAYCRELWQSLKSL
                     NIKWGCQSTLFLGDDPEMVKLAADSGCVSVFVGMESLSDDSLDETNKGFNQVRKFENQ
                     IKMFHDHGIMVNPGLVFGFDNDDESVFETAVEFLTRNKVELAYFNVLTPLPGTALFER
                     FKQEDRLIDQDWSKYDGKHVVFTPKRMTAEQLQEGFFWANHQFYSWSSIWNRLGHTRQ
                     RLIPRLEMNWEFRKLIYRTTPKGSLSPLAKVLKSLQVKLPTSETQQLIPNALHPQPSP
                     DPKPQELYLKMKARRHDAFAALFIELDGTLDHLNAKELLKRIQQAGRAAKMDIVVNFE
                     NLKQATPKAIYTLLDGEILQAILPHTKLRYRNLTKAFQAIVSEIALDHFDLFDEDIQH
                     A"
     gene            2707..3969
                     /gene="lysA"
                     /locus_tag="PKM72_08595"
     CDS             2707..3969
                     /gene="lysA"
                     /locus_tag="PKM72_08595"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNP60883.1"
                     /translation="MHDFHYQGDELFCEEAPIRQITEQVGTPCYIYSHRTLIRHFQAF
                     DQAFKAIPHIVAFAMKSNSNLTVLRLLAKEGSGADIVSGGELFRALTAGMAPNKIVFA
                     GVGKSKEEIRYALQSEILMFNVESPGELQQINEVAGSMGVRAKVALRINPDIDPQTHP
                     YISTGLKKSKFGIGADRALAEFDAAESLPHIEVVGVHSHIGSQLTQITPFVDALKKAI
                     ALIQTLQAKGLQIQYLNIGGGLGITYSDETPPHPKELAAAISPLLQSVSCKIIMEPGR
                     SIVGNAGILVTKVLYNKESADKHFVIVDAAMNDLLRPSLYDAHHDIQPVLKKESSVVN
                     TVDVVGPICESGDFLAKDRKMHQSQPGDLLAVMSAGAYGFTMASNYNSRPRVPEILVK
                     GKEITVIRKRESYEDLIRGESISDAFSN"
     gene            4067..4939
                     /gene="dapA"
                     /locus_tag="PKM72_08600"
     CDS             4067..4939
                     /gene="dapA"
                     /locus_tag="PKM72_08600"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNP60884.1"
                     /translation="MFSGSIVAIVTPFSKGKFDEKAMADLIETQIASGTHGIVPCGTT
                     GESATLTPEEHERVVSVTVEVVNKRVPVIAGTGSNSTDEAITFTKHAKAVGADAALLI
                     TPYYNKPPQEGLYRHFSAIAKAVDFPQILYNIPGRTSINMLPATVSRLSEIPNIIGIK
                     EGSGSLQQVSEIIQRSRPDFLVLSGDDPLTLPMMVLGGKGVITVTANVAPTAMANMVN
                     AALKGDYETARALHFKLSPLFGSLFLETNPIPVKAALAMMGIMSEEVRLPLTPLSPEF
                     RPALQEALKQAGVL"
     gene            4953..5756
                     /gene="dapB"
                     /locus_tag="PKM72_08605"
     CDS             4953..5756
                     /gene="dapB"
                     /locus_tag="PKM72_08605"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNP60885.1"
                     /translation="MTRTIITGAAGRMGTRLIALTQDTPGLQLAGAVEVKGHPAIGKD
                     AGEVSQIGRINIPITDDLQACLSQGDVVVDFTAPSSCLVNLQHIVNAAKSMVIGTTGF
                     SEQELAKLKMLAIKIPCVFSPNMSVGINVLLGTVGKIARSLGEQYNIEVIEAHHNKKK
                     DAPSGTALKLAEALAEGMEWDLQEVGVYTRHGITGERQTREIGMQTIRAGDIVGDHTI
                     LLGGPGERIEITHRAHTRDTFAQGALRAAEWVANKPPGLYSMADVLGLK"
     gene            6112..6348
                     /locus_tag="PKM72_08610"
     CDS             6112..6348
                     /locus_tag="PKM72_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60886.1"
                     /translation="MDMFGKIPLLEIPIHVTPDQKKWLDRMVEEGKIAIPPGGTLEKG
                     SVISMFMRMLIHNAMEEQMRQEAIDAEDEDDDDE"
     gene            complement(6756..8264)
                     /locus_tag="PKM72_08615"
     CDS             complement(6756..8264)
                     /locus_tag="PKM72_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNP60887.1"
                     /translation="MSMATSTEQLHPDVQSFVSHPRKMLINGQWADAQSGKTFSTFNP
                     ATGEVIAEVAEGDKPDIDLAVRAARNAFEQGPWRKMTPSDRGKLLWRLGDLLESNLEE
                     FAQLESLDNGKPLTIARAADVPLAVDLFRYMAGWSTKIEGNSIPISVPYMPGSQFLAY
                     TLREPVGVVGQIIPWNFPLLMAAWKLGPALAVGCTIVMKPAEQTPLSALRLGELFLEA
                     GFPEGVVNVVPGYGETAGAALAAHPDVDKIAFTGSTEVGKLIVNAATGNLKKVTLELG
                     GKSPSVVLPDADLEIGIPGVASAIFFNHGQCCAAGSRLYVEKGIFDKVVAGVADQAKQ
                     IKIGSGLDLTTQMGPLVSDEQQHRVLGYLESGFAEGAQAVAGGKRFGDKGYFVEPTVL
                     VNTKSTMKVIQEEIFGPVVCAEPFTNVEDVIAKANDNIYGLAASVWTKDLSKGHRIAA
                     QLRAGTVWINCHNVFDASLPFGGYKQSGWGREMGHEALNLYTEVKSVCAKLA"
     gene            complement(8601..9887)
                     /gene="hemL"
                     /locus_tag="PKM72_08620"
     CDS             complement(8601..9887)
                     /gene="hemL"
                     /locus_tag="PKM72_08620"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNP60888.1"
                     /translation="MKTTRSRALFQRANSYIPGGVNSPVRAFRSVGGDPLFIKEAKGI
                     LLEDVDGNKFLDYVLSWGPMILGHAHSQVLKAVISATKRGTSYGAPCEGEVDLARQIA
                     KMVPSIEQVRLVNSGTEAVMSAIRLARAYTKRDSILKFDGCYHGHADHLLVKAGSGVA
                     TLGIPDSPGVPESFARHTLTAPYNDIKATEKLIHKHANNLGAIIVEPIAGNMGVIPPS
                     DEFLPWLRMITKKYEILLIFDEVITGFRVNAGGAQALYKVIPDLTILGKIIGGGLPIG
                     AYGGSRDIMQMIAPAGPVYQAGTLSGNPVAVAAGLETIRLLQSPGVYKDLEKRSKELA
                     EGLGEAAKQAKISYYQTRVGSMLGGFFSGGPVNDYAGAKKSDTVRYAKFFNGMLNHGI
                     YLAPSQFEAMLLSTAHTKRHIQQTIAAAQKVFPTLR"
     gene            complement(10684..10830)
                     /locus_tag="PKM72_08625"
     CDS             complement(10684..10830)
                     /locus_tag="PKM72_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60889.1"
                     /translation="MKMLLSALEEIADQKKEDSLLLSPSSTKGINLILGVECLDFVIV
                     PAVE"
BASE COUNT         3120 a         2779 c         2353 g         2821 t
ORIGIN      
        1 agcctcctga ccaccttaaa aggattgcca ctcagttaca atcgagacct tcaggaagat
       61 aaagaaccat tgtttgacac attagatacc gtaaccacat cggttaaaat ctacgcagaa
      121 ctcctggctc aactcacgat acgacctgat tccatgaaag aagccgtgtc tgagggtttt
      181 ctgctggcaa cggagttggc ggactatttg gttaaaaagg gaatgccttt ccgtgaatct
      241 caccacgtgg tgggaagtct ggtacgggag tgtttagcta aagggaagga tttagggcaa
      301 ctcacgcaac aggacctagt gaacgcctca tcgtcttttg atgccaaggc ctttcaagtt
      361 ttaactcccg aagcggcaat taacagtaaa aacattatag gtggtaccgc tacgccccaa
      421 gtcacaaaac agatcaatgg ttggaagaaa tcattacagg ccgaaatgaa acgttccatg
      481 aaaaaaacgc gaacggcatg agtcatattt ttaggacttc ttcccagtat ttcctccctt
      541 tttgcatgct ggtcctgctc ttttccccaa taggttgcgg ggcggtcggc cctcccattc
      601 ctcctgaaaa ggttggcatt gaagcgaaga tactcgaaca acagcgagag caggccagaa
      661 tagagggagg cattgacgaa gacccctcga atgtaccgct ggaggaggca gttgaattac
      721 ccaccgtgta tcctatcgat acccgataat atgcagggat ttgttcagga agaacctcgc
      781 agagtagaat agaatccccc cacagattat ccatctgttt gtggaggata aaaataatgg
      841 atacccctct cactcacagt gaaattcggc ccaaacttct gttgcttgat ccctatccac
      901 gaaataatcc ctatcgcatg agcgcaagcg aacgccggtc gatctggttt ccgaagctca
      961 gcctaccagc gattgcagcc tatactccga caacatggga tgtcgaaatc gtggacgaag
     1021 ccgttatgga tatcaatttt gagactccct gtgatgtggt tggaatatcc atcatgacgt
     1081 gttatgcccc gagggcttat gaaatcgcgg atgaatttcg tcgacgtcgg aaaccggtca
     1141 ttctcggagg ggtccatccc acctattgcc cagatgaagc ccttcgtcat tgtgatgcta
     1201 ttatctgcgg ggaagccgaa gacctttggc ctcaagtcat cacggacatc gaagccgggg
     1261 ccttgaaacg aatctatcgg atggaacaat ttccaaccct gagtcactac aaaccacccc
     1321 ggattgaatt gctcagccct gattcttata tgaccaggct atgtaccttc acgacgcgag
     1381 gatgtcattt tgattgcgaa ttttgcagtg tttcaccatt caatggcaaa accactcgac
     1441 gccgacccgt tcccgaagtc atcgaagagc tgaagcgagc caaagaatgg ctgcgttcag
     1501 acattgtcga acgcatgact cacggatcac tgttccatgc cttaacgact tccctgaaaa
     1561 tttggatagg actggaagaa ggatcgatcg ttgcttttgt tgatgacctg cacaacagtc
     1621 atcgagccta ttgccgtgag ttatggcaat ccctgaaatc tctcaatata aaatggggct
     1681 gccaatccac cctcttcctt ggcgacgatc ctgaaatggt caaattggcc gccgatagtg
     1741 gttgcgtatc ggtgtttgtc gggatggaat cgctgtctga tgattcactt gatgaaacca
     1801 acaaaggatt caatcaggtc cggaaatttg aaaaccaaat taaaatgttt catgaccacg
     1861 gcatcatggt gaaccccggg ctcgtttttg gatttgataa tgatgacgaa tccgtattcg
     1921 agaccgcagt ggaattttta acgcgaaaca aagtcgaatt ggcctacttt aatgttttga
     1981 caccccttcc cggtacggcc ctgtttgaac gcttcaaaca ggaagaccgg ctcattgatc
     2041 aagactggtc caaatatgac gggaaacatg tggtcttcac acccaagcgc atgacagccg
     2101 aacaattaca agaagggttt ttctgggcca accaccagtt ttattcatgg tcctctatct
     2161 ggaatcgcct ggggcatacc cgccaacgac tgattcccag gcttgaaatg aattgggaat
     2221 ttcgaaaatt gatataccgc accacaccca aaggctcgct ttcccctttg gctaaggtcc
     2281 taaaaagcct gcaagtcaaa ctgccaacct ccgaaacaca gcaactcatt cctaacgctc
     2341 tccaccccca accctctcct gaccccaaac cccaggagct ttacctgaaa atgaaagctc
     2401 gtcgccacga tgcctttgcc gccttgttca ttgaactgga cggaacctta gaccacctga
     2461 acgccaagga actactgaaa cggattcaac aggctgggcg ggcagcaaaa atggatatcg
     2521 tggttaactt tgagaaccta aaacaagcga ccccaaaagc tatttacaca ttattggatg
     2581 gagaaattct ccaggccatc ctcccccaca caaagttacg gtatcgaaat ttaacaaaag
     2641 cctttcaagc aattgtttca gaaatcgctc tcgatcattt tgacttattt gatgaggata
     2701 tccagcatgc atgattttca ctaccaaggg gacgaattat tttgtgaaga agcccctatt
     2761 cgacaaatta ctgaacaggt cggcactcca tgttacattt atagtcaccg gaccttgatt
     2821 cggcattttc aggcttttga tcaggctttt aaggctattc cgcatatcgt agccttcgcc
     2881 atgaaatcga attcgaactt aaccgttctg cgacttcttg ctaaggaagg gagtggcgca
     2941 gacatcgtgt cgggaggaga actctttcgc gcattaacag ccggaatggc accaaacaaa
     3001 atcgtctttg ccggggtcgg gaaatctaag gaggaaattc gatatgcatt gcaatcggaa
     3061 attctgatgt tcaatgttga atctcctggt gaactgcaac agatcaatga agtggccggc
     3121 tccatggggg tgcgcgccaa agtggccttg cgtatcaatc cggatattga tccccaaacc
     3181 catccgtata tttctaccgg gctaaagaaa agtaaatttg ggattggggc agaccgggct
     3241 cttgccgaat ttgacgcagc agaaagcctt cctcatattg aggtcgtcgg tgtgcactca
     3301 cacattggct cacaattaac acagatcacc ccatttgtag atgccttaaa aaaggctatt
     3361 gctttaattc agacgctcca agctaaaggc cttcagatcc aatacctcaa cataggcggg
     3421 ggactgggca ttacctactc ggacgaaact cctcctcatc caaaagaact ggctgcggcc
     3481 atatccccgt tgcttcaatc cgtttcatgc aaaatcatta tggagccggg ccgatccatc
     3541 gtagggaacg cgggcatatt ggtgaccaag gtactgtaca acaaagaaag cgcggacaag
     3601 catttcgtca ttgtcgatgc cgcgatgaat gatctcctac gaccaagctt atatgatgcc
     3661 caccacgata ttcagccggt attaaaaaaa gagtcgtcgg tggtcaacac cgttgatgtc
     3721 gtgggaccca tttgcgaatc aggagacttt ttagcaaaag atcgaaaaat gcaccaatct
     3781 caacctggag atctccttgc cgtcatgagt gcaggagcgt atggtttcac gatggcatca
     3841 aattataatt cccggccgcg cgttcccgag atactcgtaa aagggaagga gatcacggtc
     3901 attcgaaaac gcgaaagcta cgaggacctg atccggggtg agtccatttc agatgccttc
     3961 tcgaattaat ttttcgaact cattttatgt tgattcacca aagcgctctt ggtttttcta
     4021 gttaaagaac accaaattca ctacaccaga tattgaggca atcattatgt tcagtggttc
     4081 catagtcgcc attgtcaccc cattttcgaa gggaaaattt gatgaaaagg ccatggccga
     4141 cctcatagaa actcaaattg cttcaggaac tcatggcatt gtgccttgtg ggacaacggg
     4201 tgaatcagcc acgttaactc ctgaagagca tgaacgggtc gtgtcggtca ccgtcgaagt
     4261 agtcaataag cgggttcctg tcattgcggg aacaggctca aactcaaccg atgaagcgat
     4321 tacctttacc aagcacgcaa aagccgtcgg tgccgatgcg gcattgctca tcactccgta
     4381 ttacaataaa cctccacagg aaggactcta tcgtcatttt tccgctatcg ccaaagccgt
     4441 ggactttccc caaatcctct ataatattcc agggaggacc agcatcaata tgttgccggc
     4501 cacggtgagc cgtctgtcag agatcccaaa tattataggc atcaaagaag gcagtggatc
     4561 gctccaacag gtatccgaaa tcatacaacg atcccgccct gacttccttg tgctttccgg
     4621 cgatgatccc ttgacactcc ccatgatggt cctgggcgga aaaggcgtga tcaccgtcac
     4681 cgcgaacgtt gcccccaccg ctatggccaa catggtcaat gcagcactaa aaggtgacta
     4741 cgaaacagct agggctctgc acttcaaact ctctccctta tttgggtcat tatttttgga
     4801 gacgaatcca atcccagtga aagcggccct agccatgatg ggaatcatgt cagaagaggt
     4861 tcgactgccg cttaccccgc tttcccctga atttcgacca gcactccagg aagccttaaa
     4921 acaagccgga gtgctctgag gaaatagaac acatgactcg aacaatcatt accggcgcgg
     4981 ccggccgaat gggcacgagg ttaatcgcat tgactcaaga cacccctgga ctccaacttg
     5041 ccggcgccgt cgaagtaaaa ggacatccag ctattgggaa agatgcaggc gaagtctctc
     5101 aaattggacg aatcaatatc ccgatcaccg atgatttaca ggcctgcctc tctcaaggag
     5161 atgtggtcgt cgacttcacg gctccttcat cttgcctggt caatctccaa cacattgtca
     5221 atgccgccaa atccatggtc attggaacca caggtttttc tgaacaagaa ttggccaaac
     5281 tcaaaatgct tgctatcaaa atcccctgtg tgttctcccc caacatgagt gtaggcatta
     5341 atgtgctcct cggcacggtc gggaaaatcg ccaggtcctt aggagagcaa tacaacattg
     5401 aagtgatcga agcccatcac aacaaaaaaa aggacgcccc cagcggaacc gcgctgaaac
     5461 tcgctgaggc tttagcggaa ggaatggaat gggatttaca agaagtaggc gtctatacac
     5521 ggcatggcat caccggcgaa cgccaaaccc gtgaaattgg gatgcaaacc atccgcgcag
     5581 gggatatcgt tggcgaccac accattttat tgggtggacc aggcgaacgc attgaaatca
     5641 cccacagggc ccacacccgc gatacctttg cccaaggcgc gctccgcgca gcagaatggg
     5701 tggccaataa accacctggt ctctattcca tggccgacgt cttaggctta aagtaaacca
     5761 actttcttaa tttttcagtg atggtggcag gtaatccgat agccatctcc gtgtcctatt
     5821 tatccatcat tttctttcaa caacaatagc gtttgtcccc tcacctttca caatgtcccc
     5881 aagtcgataa accagacgat gctcatccaa gcctaaagag cttgccctaa actgacgata
     5941 cccgactctt tcattgacaa agtcagagag cacatggtct tatcgaggaa ggcttgcgaa
     6001 cgatatgaat tctcaacgcc actctcttga atgctctaag agcgttggat acccagggaa
     6061 gacgagtgag ccatttcatc agacttattg aatcataagg agactgtttt catggatatg
     6121 tttggaaaga ttcccctatt ggaaattccg attcatgtga ccccggatca gaagaaatgg
     6181 ttagacagaa tggttgagga aggtaaaatc gcaattcctc caggcgggac tcttgaaaaa
     6241 ggctcggtaa tttccatgtt catgaggatg ctcatccaca atgccatgga ggaacagatg
     6301 cgccaagagg ctatcgacgc cgaagacgaa gatgacgatg atgaatagag cgaactactc
     6361 tcctccgtat tggtgactca tttctttcca agcacatcca accaatgcac gctgtgaaac
     6421 agcggagagc ataaacaaag ccgattgtcg taatttacca ttttttattt ttcttcattt
     6481 gtcacagaaa agttctcaat ggctcttggc catgaatgga cgtcctgaga aagcaatcga
     6541 taggttccaa gagatgagga gggctcaaat cccttctcaa atacttatta gtactttccg
     6601 ctcctccatt tgtcttttcc tggggcgatc caggaacaaa cgaatcttcc cgctttaatt
     6661 caccccctca tcaatcgcaa ttctgcaaat ggaatatttc tcaaacgttt ttggatttac
     6721 tcgcctgaaa tttaaacgga ttaaatagtc atttcttatg caagtttggc acagacggat
     6781 ttcacttccg tatagagatt caaggcctcg tggcccattt cgcgtcccca gccggattgt
     6841 ttgtacccac caaaaggaag tgacgcatca aaaacgttat gacagttaat ccaaaccgtt
     6901 ccggcccgca attgtgcggc aatgcgatgc cccttactca aatctttagt ccataccgat
     6961 gctgccaatc catagatatt atcattggcc ttggcgatga catcttccac atttgtgaag
     7021 ggttcagcac aaaccacagg gccaaaaatt tcttcctgaa taaccttcat ggttgatttg
     7081 gtattgacca agacagtcgg ttcaacaaag taccctttgt ctccaaaccg tttcccaccc
     7141 gcaaccgctt gagctccttc ggcaaatccc gactccaaat atcccaggac acggtgttgc
     7201 tgttcatcag aaaccaacgg tcccatttga gtggtgagat ctaatcccga gcctatctta
     7261 atttgtttgg cttgatcggc aacccctgcc acgaccttat caaaaatgcc tttttccaca
     7321 tacaaccgtg agcctgcggc gcaacattgc ccatgattaa agaaaatcgc actcgcgact
     7381 ccaggtattc caatttccag gtcagcgtcc ggcaagacga cacttggtga tttgccaccg
     7441 agttccaacg tgactttttt aagatttcct gtcgcggcat tcacaataag tttgccgact
     7501 tcggtcgatc cggtaaacgc aattttatcc acatcaggat gtgcagctaa ggccgcacct
     7561 gcggtttcac catagccggg aacaacgttc acgactcctt cgggaaaccc tgcctccaga
     7621 aacaattctc ccaaccgcag cgctgacaaa ggagtttgtt cagccggttt catcacaatg
     7681 gtgcatccca cggccaaagc cgggccgagc ttccatgccg ccatcaatag aggaaaattc
     7741 cagggaatga tttgcccgac aactccaacc ggctcacgaa gtgtgtaggc caggaattga
     7801 gacccaggca tgtagggaac tgaaatggga atggaattgc cctcaatttt tgttgaccat
     7861 cccgccatat atctaaataa atcgacagcc aacgggacgt cagctgctcg cgcaatggtt
     7921 aacggcttgc cgttatcaag agactctaac tgggcaaact cttccagatt cgattccaga
     7981 aggtccccca acctccaaag gagtttgccg cgatcagaag gcgtcatttt tcgccaaggg
     8041 ccttgttcga atgcatttct cgccgctcgc acagctaaat caatatctgg cttatcgcct
     8101 tccgccacct ccgctatgac ttcacccgtt gcaggattaa acgtggaaaa tgttttgcct
     8161 gattgagcgt ccgcccactg gccattgatc agcatttttc gtgggtggga cacaaaagat
     8221 tgtacatcag gatgaagttg ttcggttgaa gtcgccatac tcatcgggta cctccctttc
     8281 tccaaaagaa attcacctgg ggcagatttc aagacttagg gctatgaatt ggcttaaaaa
     8341 cctaaatgag tagccaatct aatattgtcg ctggctcccc attatctctt attatgacga
     8401 cgccgcaatt tctaacgcaa attttgttcg gttccataaa tacttgacca ctacaattgc
     8461 ccctggatct gcaatcagga caatagataa aagccctggc tcggttatcc tgttcaaagg
     8521 caagttggga aactatgggg ttatctggat tggcctatcc aaatgccaat ccagaacagg
     8581 gcttttcaaa aacaaagggg ttatcggagg gttggaaaga ccttttgtgc cgcagcaatt
     8641 gtctgttgaa tgtgcctttt ggtatgagcc gttgaaagta acatggcttc aaattgagag
     8701 ggagccaaat aaattccatg attaagcatt ccattgaaaa acttagcata tctcacggta
     8761 tctgattttt tggctcccgc gtagtcgttg acaggtcctc ccgaaaaaaa tccacccaac
     8821 attgatccga cccgtgtttg atagtaggaa atttttgctt gctttgccgc ttcacccaag
     8881 ccttcagcca gttccttgga tcttttttct aaatctttat agacaccagg gctttgaagg
     8941 agtcggatag tctctagtcc agcagccacg gccactggat ttccggagag agttccggcc
     9001 tgatacactg gtcccgccgg ggcaatcatt tgcataatgt ccctggaccc tccatacgct
     9061 ccaatgggta agcccccacc gatgattttc cccaaaatcg tgagatctgg aatgacttta
     9121 tagagggctt gcgctccacc ggcattcact ctaaatccgg taatgacttc atcaaaaatc
     9181 aagagaatct cgtacttttt ggtgatcatt ctgagccagg gcagaaattc atccgatgga
     9241 ggaatcactc ccatattccc tgcaataggc tccacgataa tggcgcccaa attgttggca
     9301 tgtttatgta tgagtttttc ggtagcttta atatcgttat agggcgcagt caaggtatga
     9361 cgggcaaacg attctggtac accagggcta tctgggatcc ctagggtagc cactcccgat
     9421 ccagctttta caagcaaatg gtcggcatga ccatgatagc agccatcgaa tttcaaaatg
     9481 gagtcacgct tagtgtatgc cctcgctaaa cggatggcgc tcataacagc ttccgttcct
     9541 gaattaacga gacgaacttg ttcaatcgaa ggaaccatct tggcaatttg cctggctaaa
     9601 tccacttctc cctcgcaggg cgccccatag cttgtcccgc gtttggtagc gctgatgaca
     9661 gcctttaaca cctgagaatg agcatgaccg agaatcatag gtccccaaga taaaacataa
     9721 tccaaaaatt tattgccatc cacatcctct agcaaaatcc ccttagcttc cttgatgaat
     9781 aaaggatctc ctccgaccga gcggaatgct ctgacgggac tattgacacc accgggaata
     9841 taggaattag cacgttgaaa taatgcccga gaccttgttg ttttcacaga cacactcctc
     9901 caatgtttca aagtaattta atgtcccaat ttggcaattg ttatcacaaa aggaaatgca
     9961 ccaccaaatt ctcaataagc agtcccagcc cagtccttca ggaaatacgt ccgaattata
    10021 tgaaaaatca aaatttccct taattaatta aaatacttca atataaattt cctttaattt
    10081 cactctaatt tttaccggat ctccaaagcc caactatata ttttttggga aacggcggat
    10141 cccgtaaggc cccaaacact tccaaataat ttcatctgat taatacagga aatttccaac
    10201 aatataggga ataccaccaa aagatcccac ttaattccca taaaaaaaat ccctcaaatt
    10261 ctgattctcc aaatgcaccc aattttctct ttcttaaccc caacaggata gcacttcaaa
    10321 agtttcaaca ctaaagctct aataatctag caaactccaa ttcaaaataa attaggtatt
    10381 tgttccgaat aaaatcctca ttaataattt aacaagaata tgctcccaat acatatgaga
    10441 aagcataaat tcgttccata agaaagtgtc aaaatagatc ctctaaaagt ccaaactatt
    10501 ctcattaata aaagagaatt ttttacctat ttatttttta aacttttaac cgaaaaattt
    10561 atattagtgg taacaattct tgaacatatt gggatgcact gatgtattgt gaacaacgaa
    10621 ggaagaatga aataacaatt gttgattgtt ttggaaggtt tgatgaagaa gacacgaatc
    10681 aatttattca acggctggaa caattacaaa atcaaggcat tcaacaccta atattaaatt
    10741 tatccccttt gtactacttg gactcaaaag tagtgaatct tctttttttt ggtcagcaat
    10801 ttcttcaagc gcactcaata acattttcat tagtaagtcc acttagttct gtaagaaatg
    10861 aacttgtggg aggaaaagtt ccaggtacca taccaatctt tagcaccatg tatgatgcga
    10921 tacatcgccc acaatgcgct tatcaagagt gttaaattta acgaacattc cttgaacaag
    10981 aaaagcccag agatatttaa tctctgggct tttcctaatt aaatctcggc aacgacctac
    11041 tttcccacag cctcacggct gcagtatcat cgg
//
LOCUS       DAORAQ010000082        11044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_970_length_11044_cov_70.219583, whole genome shotgun sequence.
ACCESSION   DAORAQ010000082 DAORAQ010000000
VERSION     DAORAQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_970_length_11044_cov_70.219583"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKM72_08630"
     CDS             complement(<1..290)
                     /locus_tag="PKM72_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNP60890.1"
                     /translation="MGNLLVVGSVAFDSVRTPFGEASDVLGGSATYFSTSASFFTDVN
                     LIAVVGEDFPKEHLDFLRSRGINLDGLEQRPGKTFRWRGEYSYQLNEAQTLET"
     gene            complement(296..1216)
                     /gene="mtnP"
                     /locus_tag="PKM72_08635"
     CDS             complement(296..1216)
                     /gene="mtnP"
                     /locus_tag="PKM72_08635"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNP60891.1"
                     /translation="MNIKRPSSPTQRAQIGIIGGSGLYEMDGFTNLKEVRMATPFGKP
                     SDAIILGTFHGIRVAFLARHGRGHRTLPSAINYRANIHALKALGVTRVFSISAVGSMK
                     ESIQPGDFVLPDQFIDRTTQRDNTFFDQGIVAHVAFADPLCGTLSNVLEKASEGVSVT
                     VHRPGTYLCIEGPQFSSRAESFLYRQWGVDVIGMTNIPEAKLAREAELCYATLALVTD
                     YDCWHETEEAVSVGAILSIMHQNVETAKLVLRQALDLAKDLGPCSCQTALEHAVITPL
                     KQMSPTLKKRYQLLLRRHLLSTTLHSKKRS"
     gene            complement(1372..2397)
                     /gene="miaA"
                     /locus_tag="PKM72_08640"
     CDS             complement(1372..2397)
                     /gene="miaA"
                     /locus_tag="PKM72_08640"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNP60892.1"
                     /translation="MKQTDIITAWKPIVAIVGPTAIGKSRIGIEVAKILHTEILTADS
                     RQVYRGMDIGTDKPSLAEQQNIPHRLIDLVDPDQSFNAGDFRHHATQDIARLHHLGLL
                     PLVIGGTGLYIRALLRGLCPGPPANWIIRSELAQEAKAQGPAFLYKKLQQVDPELAQR
                     LHPNDQPKVQRALEVYRILGTPLSVIQQQHRFDDAPYPYLLIGLTMERQTLYQRIETR
                     VDWEIQKGLVEETQSLMNQGFSRDLGSMKGLGYRQFSGYLAGDYSYEEAVRLLKRDTR
                     HFAKRQMTWFQKESGIQWITLEGSDNPHRAATKIVDHINQFLYTFARLPFPAEPTPDL
                     KSTQSSG"
     gene            complement(2418..4211)
                     /gene="mutL"
                     /locus_tag="PKM72_08645"
     CDS             complement(2418..4211)
                     /gene="mutL"
                     /locus_tag="PKM72_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNP60893.1"
                     /translation="MSSTVTGKVHILPDSVSCRIAAGEVVERPASVVKELIDNSLDAG
                     STLITVEVEEGGRRVIRVMDNGAGMTRMDAQLACQRFATSKLRSEEDLLTLLTMGFRG
                     EALPSIASVSRFFLKTVSPESPLGTQTQSMGGVTWEVCDYTGPLGTQVEVRDLFFNTP
                     GRLKFLKTVPTEFSKICYVVQQAAAVHPGIHFRLRHQNHTVLEYPAVSSLQDRLLQIY
                     GPDFLERFLPVTYNAGSFQLTGFTVSPHHARTSRAPQEIFVNGRPIKNTTISHAVHEA
                     YGSFLPKGKHPQFIVFIHLDPQSIDINVHPTKREVRFSHPEFVHMGVLRGIKALFFSQ
                     PSPDIISDEHKETPGPVTPHLPRTTPGSMNVAPASFQHGSEFPRTGTNRPYPLSLFVQ
                     EPPSVYGSREQPCEVHSLGQIHHTYLLGQIDQDLFIVDQHTAHERVRFERLLRSWKKK
                     EILQQSLLIPEPVELLPHQGELLEEWLPFLAQAGLDVERFGTTSYVVRAIPAMLGNIS
                     VGSLILELLDELSEWQSTDALDNTIKPILATMACQSAVQAGRPMTQAEITILLQDWAQ
                     ESFPMTCPHGRRVAIKHSLEELHTIFARPLK"
     gene            complement(4270..5166)
                     /gene="ybgF"
                     /locus_tag="PKM72_08650"
     CDS             complement(4270..5166)
                     /gene="ybgF"
                     /locus_tag="PKM72_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNP60894.1"
                     /translation="MTHTFSKTAIFIGLSLTGGWGCATEPNLTDIQKQVQTLLVQQEA
                     SHKQEKQIIDRMETVESQLDEHDFLVGELIKTEEEASLDTRHLLEKLERTSALLREQI
                     EQTRSTTQRRDQDLSIRVKALESRIDNVIHQPRSVSESPESTTPNPANPASNPKEAQT
                     GLPASPEERAGAENEASAFRSAYKVFLNGNYDRASVEFKRFVTNYPSTTLTPQAYYYL
                     GETFYVQKHYDAAKQALEQVISRYPSSKYRSHALYKLGQIMLEIDQRSKAQELWNSII
                     QDYPDSPESTQAKEQLKKSGLS"
     gene            complement(5325..6995)
                     /locus_tag="PKM72_08655"
     CDS             complement(5325..6995)
                     /locus_tag="PKM72_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60895.1"
                     /translation="MAQQADLARIQKDLELQIAKIKDEKKALGLQVDETKAQLIKMNE
                     EAQKTRGNLASINQKATLLQEKDVASLYGKLEETEKSLQDLKKDYTNQTDTLKSDVQS
                     IQSTIQAQGEDLQTVKTHNTTLAQQVDENNQALTTNMGEFQNSLSQFKDTLTSLGTTI
                     GQVQTDLSGQRQDLGVVQSRTEKLSGSILQVQGGIEKSGELLSARLDEQSRQVTQLEN
                     QVTILQDKLAADTQALRTYLEQDVNAAMAQLVAEINTQQAPKLAQIDSLQKDMEALGT
                     HVQADASHVQDLSQSVLKLREAQEVMGSLLGKRGDEIIQQAGRLSERMNTVESHQTAL
                     TEQLQSNTQKTSAHLVEVNASLNSITQALDQTRQSLSSRLAKQEEAGQVLNQSVQQFQ
                     QLKRDVQDQKQQLQTVNQLVDQLNQTADHIGSRLKDLETHQSGLVGKIDSDTQMTNTH
                     LQEVNSGIQSVAQALENVSTKLNARIENQEQRLNRAMTSFQQVQGTADTSQTNLTHLN
                     QLTETVNKLRDVINTIGTKLGERVDQHEDRLGQLAQRVNRLQSPKPQK"
     gene            complement(7154..8110)
                     /locus_tag="PKM72_08660"
     CDS             complement(7154..8110)
                     /locus_tag="PKM72_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNP60896.1"
                     /translation="MRPAIRVISLLGIIGLSLVSMSGCGEKRIHVSTASGSPGEETET
                     SELDGDSAKVAGTSIDESSLPGQGASTEEVRADAVQPIAPVDPLNFGYDPNHMDQWFK
                     QNSQAGIAPEKDSEIDTSSSAGPLTPFNDGNDISTQSSSSGMNGSDQDQSGTSPFTSQ
                     TEFQNDLSNLPKDTEPIPETLQVAKAEPSEIIREQLDKIQEEELATISAGLEDVFFQF
                     DSWALTEEAKDSLERDMAWLTNDSSSLLIIEGHSDQRGTQAYNMVLGKKRAMAVRDFL
                     SQLGVDPSRLTVISYGKDKPFCQDTTEVCYQLNRRGHLLVQN"
     gene            complement(8155..9513)
                     /locus_tag="PKM72_08665"
     CDS             complement(8155..9513)
                     /locus_tag="PKM72_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60897.1"
                     /translation="MKNALKPPYAILALLAFSVIAVSPAFLHGEEADLKATRSEFQKI
                     PIWVMGFSPVQRDTGLSEDIETQVPSVLKADLKRSQIFSIAELPPAKGDFSDNKCMSL
                     SSNLAPHFQKVTVSTWGRIGVGGTSNGVQGLILDACAFDPGQQEVLTGKRYFSLKTTE
                     TLTRLMAHRWADELVYRYTGEQGIARTKIAFVGQKENGRELFVMDYDGYDPQQVTADG
                     YLNLMPTWAPDRKSLIYTAYRDRKQLIMKRELATGREEILVSPASLNITASFAPNGKS
                     IAYSAAQDGNSDLYQIELDSRSVKQLTSHNSADLSPSWSPDGRHLAFTSDRGGRPQIY
                     IMDAEGLNVRRLTFDGDYNAAPAWSPKGDWIVYVCRVPSEGFKLCRISPNGEQRKQIT
                     NGQSIDDSPSWAPNGRHIVFSSIRRGESQLYIITSEGAGLEKISSAGEHLSSPSWSPF
                     QP"
     gene            complement(9534..10904)
                     /locus_tag="PKM72_08670"
     CDS             complement(9534..10904)
                     /locus_tag="PKM72_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNP60898.1"
                     /translation="MMALFLRFQSTVEEPFRAIEVNLISLPEEQAPDSSQKKSSPPAP
                     SQQKSQPTKAEPIKTEPKEAALPPLPVPTPSERLSEFLEGAVGSIVVPHKQEMATPAP
                     SPPTEQPPLNDQSPLFENLRMPPIAPQISRPERLHSSQPLVAPKPNATPSPKQATPPT
                     PVPPEPQDSVSQPQPPKIEPTVKLAPVLPELSSVTPFKMSEKERQPKDASPPSNIEES
                     LKRTLPTIPTLAPTRKIKKQTKASTPQQEKPFTPGLSAPQLAQIAPSPPLEKPPQREK
                     MSDMMKQLLEEAKAPNLKLSPASPPPQEPGSSSSARTQPVQSEMDQRIAKLSIPDVTP
                     VESIKNRLQLLEVQPTSNSRNTAAQSSAGTNHYYGMIQDLIQDQWRRTPLVANAPLVV
                     LKFRIFRTGEISQIRIDKGSGNGYYDSAAQRAVNAVNPLPPFPPDISDAFLDVRFQFI
                     KTDQKD"
BASE COUNT         2546 a         2433 c         2962 g         3103 t
ORIGIN      
        1 gtttccaggg tttgagcttc gttgagttga taggaatatt ctcctcgcca gcgaaaggtt
       61 tttccgggtc gttgttctaa tccgtccaga ttgatgcctc gactacgcag gaaatcgaga
      121 tgttcctttg ggaagtcttc gccaacgacg gcaatcagat tcacgtccgt gaaaaaactg
      181 gccgaagtgg aaaaatacgt ggcggatcct cccagcacat ccgaggcttc accaaacggc
      241 gttcgtacag aatcaaaggc gacggatcct actactaata aattacccat aatgattagg
      301 accttttctt ggagtgtaag gtggtcgata gtaaatgacg ccgtaacaga agctgatacc
      361 gctttttcaa ggtcggactc atttgtttaa gcggggtgat gacggcgtgt tctaatgcgg
      421 tctgacacga gcaaggccct aggtcttttg cgagatcgag tgcctgccga aggacgagtt
      481 ttgcggtttc gacattttga tgcatgatgg ataagatggc tccaacggac acggcttctt
      541 ctgtctcatg ccagcaatca taatccgtga ccagcgcaag ggtggcatag cacagttcgg
      601 cttcacgagc caatttggct tccggaatat ttgtcatacc aataacatcc acaccccatt
      661 gccggtagag aaaagattcc gcccgactgg agaattgtgg cccttcaatg cagaggtacg
      721 taccaggacg gtgcaccgtc acggagactc cttcgctggc cttttctagc acattcgaca
      781 aggtcccaca caggggatca gcgaaggcga catgggcgac gatgccctgg tcgaaaaacg
      841 tattgtcacg ttgagtggtc cggtcaatga attgatcagg caggacaaaa tcccccggtt
      901 gaatggactc cttcatgctt cccaccgcac tgatggaaaa tacccgcgtt acacccaggg
      961 cttttagggc atgaatattc gcgcgataat ttatggcgga agggagagtg cggtgtcctc
     1021 gaccgtggcg agccaaaaag gcgacgcgta tgccatgaaa ggttcccaga attatggcgt
     1081 ccgaaggttt cccaaacggg gttgccattc ggacttcttt gaggttggtg aatccgtcca
     1141 tttcatagag tccgctgcct ccaattatcc cgatttgagc ccgttgggta ggggaggatg
     1201 ggcgtttaat attcattcac tcgttctccg ccatgtgagt aggtctctct gatcaaaagg
     1261 gaagggcatg tggtttcaat cgtatgctga ttggaggtcg aactagtgag gagtttttct
     1321 gtcccaaaag agtgaaattt tccgcattgt atcatgaact gccctgtatg gttaacctga
     1381 cgattgggtg gattttaaat cgggcgttgg ttctgcagga aatggtaacc gtgcaaaggt
     1441 atagagaaac tgattgatgt gatctacgat tttggtggcc gctcggtgtg ggttgtctga
     1501 accttccaat gtgatccact ggatgccgga ttccttctga aaccacgtca tttgtcgttt
     1561 ggcgaagtga cgggtatccc gttttaataa tcgcacagct tcttcataag aatagtcacc
     1621 ggctaagtat cctgaaaact gtcgatagcc gagccctttc atggagccga gatctcgaga
     1681 aaatccttga ttcatgaggg attgcgtttc ttccaccagg cctttctgaa tttcccagtc
     1741 aacgcgtgtt tctattcgct gatacagggt ctggcgttcc atggtgagcc cgatcaataa
     1801 atagggatat ggagcgtcgt caaaccggtg ttgctgctga atgaccgata gcggtgtgcc
     1861 gagaatgcga tacacttcaa gggcgcgttg gaccttcggc tggtcattgg gatgtagccg
     1921 ttgtgcaagt tcggggtcca cctgttgaag ctttttataa agaaaagccg gtccctgagc
     1981 cttggcttcc tgggctaatt cactccgaat aatccaattg gccggtggac ctgggcaaag
     2041 acctcgtaag agggctcgga tatagagtcc ggtgcctccg atgacaaggg gcaggagacc
     2101 taggtgatgc aggcgagcaa tgtcttgggt cgcgtgatgc cggaagtcgc ccgcgttaaa
     2161 ggattgatct ggatcgacga gatcaattaa gcgatgagga atattttgct gttctgctaa
     2221 tgagggttta tcggtaccaa tgtccattcc acgataaacc tgtcgggagt ctgcagttaa
     2281 aatttccgtg tggagaattt tggcgacttc aatcccgatg cggcttttcc cgattgcggt
     2341 tggtcccaca atagcgacga tgggtttcca tgctgtgatg atgtcggtct gtttcatgaa
     2401 caatcagaga atgagcctta cttcaatggg cgggcaaata tggtgtgcaa ttcttccaag
     2461 gaatgcttaa tggccactcg cctcccatga ggacaggtca tcggaaagct ttcttgtgcc
     2521 cagtcctgta aaaggatggt gatttccgcc tgggtcatgg ggcgtccggc ttgcacggca
     2581 ctttgacagg ccatggtggc cagaatgggt ttaatcgtgt tgtccagcgc atctgtggat
     2641 tgccactccg agagttcatc cagtaattcc aggatcaggg aaccaactga tatatttcct
     2701 aacatggctg gaatggctcg cacaacataa gatgtggttc caaatcgttc cacatccaat
     2761 cctgcttgag ccagaaaagg tagccattct tccaataact ctccctggtg gggaagaagt
     2821 tcaaccggct cagggatgag caagctttgt tggagaattt cttttttctt ccatgaccga
     2881 agcagtctct caaagcgtac tcgttcatgg gccgtgtgtt ggtcgacaat gaataaatcc
     2941 tgatcgattt gaccaagaag ataggtgtga tgaatttgcc cgagagaatg cacctcacat
     3001 ggctgctccc tactcccata gaccgatggc ggctcctgga caaaaagaga aagagggtaa
     3061 ggtcgattgg tgcccgtacg agggaattcg gacccatgct ggaatgaagc cggcgcgacg
     3121 ttcattgatc cgggtgttgt tctgggtaaa tgaggagtaa ctgggccagg ggtttccttg
     3181 tgctcatcgg atatgatgtc gggtgagggt tgcgagaaaa agagagcttt aatgccacgg
     3241 agaaccccca tatgtacgaa ctctggatgg gagaagcgaa cctcccgttt agtcgggtgg
     3301 acattgatat cgattgactg gggatcaaga tggatgaata cgataaattg tgggtgtttc
     3361 ccttttggta aaaaggatcc gtaggcttca tgcacagcat gggaaatagt cgtatttttt
     3421 atagggcggc cattaacaaa aatttcctgt ggcgcacgag acgttcgtgc atggtgagga
     3481 ctgacagtaa accctgtgag ttgaaatgaa ccggcgttat aggtgacggg gagaaagcgt
     3541 tcaaggaagt ccggtccgta gatctgtaat aatcggtctt gcagtgagga tacagcagga
     3601 tactccagca ccgtatgatt ctggtggcgt aatcgaaagt ggatccctgg gtgcacggct
     3661 gccgcctgtt ggacgacata acaaatttta gaaaattcgg tgggaacagt cttgagaaat
     3721 ttcagccgcc ctggagtatt aaagaaaaga tcacgtacct ccacttgagt gcccagcggg
     3781 ccggtatagt cgcaaacctc ccatgttact ccccccattg actgggtctg cgtacccagg
     3841 gggctttcgg gggaaaccgt ctttaagaaa aaacgggaaa cggaggcgat gctgggaagg
     3901 gcttctcccc ggaaacccat tgtcaggagt gttaagagat cttcttcgga ccggagtttg
     3961 cttgttgcaa agcgttgaca ggccagttga gcatccattc gcgtcattcc tgccccatta
     4021 tccatgacgc gaatgacccg tcgaccacct tcttcaacct caacggtgat caacgtgctg
     4081 ccggcatcca gactgttatc gataagttct ttcaccacgg aggcgggtcg ttcaaccact
     4141 tctcctgcgg caatacgaca cgacacgcta tctggaagaa tgtgaacttt tccagttacg
     4201 gtcgaagaca tgggattcaa agcacaaggt attgagtgtg aaaaatagat tgcgaaggct
     4261 tgcagaggat caagacagcc cggatttttt taattgctct ttcgcttggg tggattctgg
     4321 tgaatcgggg taatcctgga tgatagagtt ccacagttcc tgagcttttg atcgttggtc
     4381 gatctcaagc attatttgtc cgagcttata taacgcgtgg ctccggtatt tgctgctagg
     4441 atagcggctg atgacttgtt ccagggcctg cttggcagcg tcatagtgtt tttgcacgta
     4501 gaacgtttcc ccaaggtagt aataggcttg aggggttaag gtggtcgacg gataatttgt
     4561 gacaaatcgt ttgaattcta ccgaggcccg gtcataattt ccgttgagaa aaaccttata
     4621 ggctgaccga aaagccgacg cctcattttc cgcccccgca cgctcttcag gggatgcagg
     4681 taatcccgtc tgggcttcct tcggatttga cgcagggttt gcagggttag gagtcgtgga
     4741 ttccggggat tccgatacag atcgaggttg atgaatcacg ttgtctatgc ggctttccag
     4801 agcctttacc cgaatggaca ggtcttggtc acggcgttga gtggtggaac gggtttgttc
     4861 aatttgttct cggagcagag cactggtccg ttccagcttt tctaaaaggt ggcgagtatc
     4921 caggctggcc tcttcctccg ttttaatgag ttctcccact aagaaatcat gttcatccaa
     4981 ttgcgattca accgtttcca ttcggtcgat tatttgtttt tcctgcttgt gggacgcttc
     5041 ctgttgaacc aggagtgttt ggacctgttt ttggatgtca gtgagattgg gctctgttgc
     5101 gcaaccccat ccgcccgtga gggataaccc tataaaaatg gccgttttgg aaaatgtgtg
     5161 agtcatgaaa taccggcctc ggctacaaaa gatgtggtat tagggaacct aattaacctt
     5221 caacttgtat cttatcaatt gccagggcgt taagacaaag ggggcatatg acggtataga
     5281 gatatactcg gcctttcctt gagaatatta agtaatgatg aagcctattt ttgcggtttg
     5341 gggctttgca ggcgatttac cctctgggcc aattgaccca gtcgatcctc atgctggtcc
     5401 actcgctcac ctaatttggt tccaatggta ttaatcacat cacgcaattt attgacggtt
     5461 tctgttaact gattgaggtg ggtcagattg gtttgcgagg tatcggcagt cccttggacc
     5521 tgttggaaag aggtcatggc ccgattcagc cgctgttctt ggttttcaat acgagcattc
     5581 agctttgtac tgacattttc caatgcttgg gcgactgatt ggattccgct gttgacttct
     5641 tggaggtggg tattggtcat ctgggtgtca gagtcgattt tccccaccag cccggattga
     5701 tgggtttcca gatcttttaa tcgtgaccca atgtggtcgg ccgtctggtt gagttgatct
     5761 acaagctggt tgaccgtctg caactgttgt ttttggtcct ggacgtctcg tttcaactgt
     5821 tggaattgtt gaacggactg attgagtact tgtcccgcct cttcttgttt ggccaggcgg
     5881 ctggagagtg attgcctggt ttggtcaaga gcttgggtaa tggagttgag gctggcattt
     5941 acctcgacca gatgagccga tgttttttgg gtattggatt ggagttgctc ggtcaatgcg
     6001 gtttggtgtg attcaaccgt attcatgcgt tccgaaagtc gtcccgcttg ttgaataatt
     6061 tcatctccgc gtttcccaag taaactcccc atgacttcct gggcttcccg aagcttgaga
     6121 actgattggg aaagatcttg tacgtgagaa gcatccgctt gtacgtgtgt ccccaaggct
     6181 tccatgtctt tttgtaggga atcaatttgc gcaagcttgg gggcttgctg ggtattaatt
     6241 tcggcaacta gctgggccat ggctgcattc acatcctgtt ccagataggt ccgaagggct
     6301 tgggtgtcgg cggcgagttt gtcctgaagg attgtgacct gattttccaa ctgagtgact
     6361 tgtcttgatt gttcatcaag tctggcactg agcaactctc ccgatttctc tattcctccc
     6421 tgaacctgta gtatcgaacc ggacagtttt tctgttcgag attgcacgac tccgaggtct
     6481 tgtcgttgtc ctgataaatc tgtttggacc tgtccaattg ttgttccgag gcttgtcagt
     6541 gtgtctttaa attgggaaag ggaattctga aattcgccca tgttcgtggt taatgcctga
     6601 ttgttttcat caacctgttg ggcaagggtc gtattgtggg ttttgaccgt ttgcaggtct
     6661 tccccctgtg cctgaatcgt tgattgaatc gattggacat cagattttaa tgtgtctgtt
     6721 tggttcgtat agtccttttt gagatcttgg agactttttt cagtttcttc caattttccg
     6781 tataaactcg ccacatcctt ctcttggagg agcgtggctt tttgattaat agaagccaga
     6841 ttgccgcgag ttttttgcgc ttcctcattc atcttgatca gttgtgcctt agtttcatca
     6901 acttgtaagc caagggcctt tttttcatct ttgatcttag caatctgtag ttctaagtct
     6961 ttttgaattc gggcaaggtc agcttgttgg gccacgcagc ctgtggccaa taaactagag
     7021 acccaacata ccactatcca cttgagagga aaagatgatg gcaactgtct tgaaacattt
     7081 cgcaaagtca gccctatttt cactctggaa ttattcctcc atgttttccg aaaaccagtc
     7141 gattgaagga ttgctaattt tgaactagta aatgtccccg tcggttcaat tggtagcata
     7201 cctctgtggt gtcctggcaa aatggcttgt ctttcccata ggaaatgacc gtcaatcgtg
     7261 aaggatcaac acccaattga gagagaaaat cccgaacggc catggctcgt ttttttccaa
     7321 gcaccatgtt ataggcctgc gtcccacgtt gatcggaatg tccttcaata attaaaagtg
     7381 atgaggaatc gttggtgagc caggccatgt cccgttcgag agaatcctta gcttcctcgg
     7441 tgagagccca actatcaaat tgaaaaaaga catcctctaa accagcagat atggtggcca
     7501 attcttcctc ttgaatttta tccaactgct ctcgaatgat ctctgacggt tcagctttcg
     7561 ctacttgaag tgtttctgga attggttccg tgtcttttgg cagatttgag agatcatttt
     7621 ggaattccgt ctgggaggtg aagggcgacg tccctgattg gtcttgatct gaaccgttca
     7681 tgcctgaaga acttgattga gttgagatgt catttccatc attaaatggg gtcaatggac
     7741 cggcactact acttgtatca atttcagaat ctttctcggg agcgatcccc gcttgagagt
     7801 tctgcttgaa ccattgatcc atatggttgg ggtcgtagcc aaaattgaga gggtcaaccg
     7861 gagcaattgg ttgaacggca tctgcccgaa cttcctcggt ggaagcgcct tgccctggca
     7921 gggaagattc atcaattgag gttcctgcta cctttgctga atcgccgtct aattctgatg
     7981 tctcagtctc ttcgccaggg gaaccggagg cggtggaaac gtggattcgt ttttccccac
     8041 atcccgacat actgacgagt gaaagaccga taatgccaag tagtgaaatc acgcgaatgg
     8101 ctgggcgcat aaaacgactc cttttttgaa ctatcacggt ggttgtttcg taaattatgg
     8161 ttgaaatggc gaccaagagg gagagctgag atgttctcct gccgaggaaa ttttctccaa
     8221 tccggctccc tcactagtga tgatgtataa ttgactttct ccccttcgaa tcgaactaaa
     8281 gacaatatgg cgtccatttg gagcccagga aggagaatca tcaatgcttt gcccgttggt
     8341 gatttgtttc cgttgttccc cattcggact gatgcggcat aatttaaatc cctcacttgg
     8401 cacccggcac acatagacga tccagtctcc ctttggagac catgctggcg ccgcgttgta
     8461 atctccatca aaggttaagc gacggacgtt caagccctcg gcatccatga tgtaaatttg
     8521 tgggcggcct ccccgatctg aagtgaaggc cagatgtcgc ccgtctggag accatgacgg
     8581 ggacaaatct gcgctattgt gcgaggttaa ctgcttcacg cttctactgt ccaattctat
     8641 ctgatataga tccgagttgc catcttgggc agcggagtag gcaatagatt ttccattagg
     8701 agcaaaggag gccgtaatgt tcaagctggc tggcgaaacc aaaatttcct cccgaccggt
     8761 agccaattcc cttttcataa tgagttgttt gcgatcacga tatgcggtgt aaataagcga
     8821 tttccggtcc ggggcccagg taggcatgag gtttaaatac ccgtcagccg tgacctgttg
     8881 cggatcatac ccatcgtaat ccatgacaaa aagctctcgt ccattctctt tttgacctac
     8941 aaatgcgatt ttcgtcctgg caataccctg ctcgccggta tatcgataga ccagttcgtc
     9001 tgcccaccga tgggccatca agcgagtgag ggtttctgtc gttttgaggg aaaagtaccg
     9061 tttcccggtc aatacctcct gttgtcccgg atcaaatgcg caggcatcca ggataagtcc
     9121 ctggactccg tttgaagtcc ctcctactcc tattcttccc cacgtagaga ccgtcacttt
     9181 ttggaagtgc ggtgccaaat ttgatgagag actcatacat ttattgtcag aaaaatctcc
     9241 tttggcagga ggtaattcag caattgaaaa tatttgtgag cgtttgagat ccgctttaag
     9301 gacggatggg acctgagtct caatgtcttc tgagagaccg gtatcccgtt ggacagggga
     9361 gaatcccatt acccaaatag ggattttttg gaattctgaa cgggtggctt tgagatccgc
     9421 ttcctctcca tgaagaaagg ctggggatac ggcaattaca gaaaaggcaa gcagagccag
     9481 gatggcatac ggaggtttga gtgcattctt cataatatag ctggtgtcct taactaatcc
     9541 ttttgatccg ttttgataaa ttgaaatcgc acatccagga aagcatcaga aatatcaggt
     9601 ggaaatgggg gtaagggatt gaccgcattt accgcccgtt gagcggcgga atcgtaatat
     9661 ccatttcctg aacctttatc aatgcgaatc tgggaaattt ctccagttcg aaaaatacgg
     9721 aatttgagca ccactaacgg agcatttgca acgagtgggg ttcttctcca ctgatcctga
     9781 atgaggtcct ggatcattcc ataataatga tttgttcccg cagaagactg agccgcagta
     9841 ttcctggaat ttgaagtggg ttgcacttcc aggagttgga ggcgattttt aatcgattca
     9901 accggcgtga cgtcgggaat ggataatttt gcaattcgtt gatccatttc tgactgcacc
     9961 ggttgggttc gtgctgaaga agaggaccca ggctcttgag ggggtggtga ggctggagac
    10021 agtttcaagt tgggggcttt agcttcttct agtaattgct tcatcatatc tgacattttc
    10081 tcacgttggg gaggtttttc cagtggtggg gatggagcaa tttgggctaa ttgcggtgcg
    10141 gagagtccag gggtgaaagg tttttcttgt tgcggcgtgg aggctttggt ctgttttttg
    10201 atttttcgag tcggtgccag cgtcggtatg gtcgggaggg ttcgcttgag cgattcctcg
    10261 atattactgg gtggagaagc gtctttgggc tgtctttcct tttcggacat cttaaacggg
    10321 gtcaccgaac tcaattccgg tagcacagga gcgagtttga cagttggctc aattttagga
    10381 ggctgaggtt gggataccga atcttgaggc tccggaggaa caggtgttgg gggtgtagcc
    10441 tgtttgggtg atggcgttgc gttaggctta ggagcgacta acggttgtga agaatgtaag
    10501 cgttccggac gcgaaatttg tggagcaata gggggcattc gaagattttc aaataacggg
    10561 gactggtcgt tcaagggagg ttgttcggtt ggaggagatg gtgccggagt ggccatttct
    10621 tgtttgtggg gcactacgat cgagccaacc gccccttcca aaaattccga taatcgttcg
    10681 gagggtgttg gaactggcaa aggaggaagg gcggcttctt tgggttcggt ctttattggt
    10741 tctgcttttg ttggctgaga tttttgttga gaaggcgccg ggggagaaga ttttttttgg
    10801 cttgaatccg gagcctgctc ctccggtaaa gaaataagat ttacctcgat ggctctgaaa
    10861 ggctcctcga ctgtggattg aaatcgtaag aacagggcca tcatgatgac gagggcatgc
    10921 agaaaaactg aggtgacgag tccccaacgg aatgaggagc tttcttgtga aacatcagca
    10981 gcgagcccaa gggaaattag acttggggat tcagcatgtc ccattggtca aacgcttagc
    11041 ggga
//
LOCUS       DAORAQ010000083        10990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_974_length_10990_cov_57.366529, whole genome shotgun sequence.
ACCESSION   DAORAQ010000083 DAORAQ010000000
VERSION     DAORAQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_974_length_10990_cov_57.366529"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            606..1607
                     /locus_tag="PKM72_08675"
     CDS             606..1607
                     /locus_tag="PKM72_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNP60899.1"
                     /translation="MTVQTLPSSVWSIVLAGGEGERIKPSIQQWLGYPIPKQYCTFVG
                     TRSMLQHTWDRADQIGVPRKKVTVVGRTHQQSLEHHCTRQDEGTLIFQPRNCDTAPGV
                     FLPLTYVKAWDPQAVVVLLPADHFICPEDRFVAAVRRAVRGVEFLSDRMILMGVRPSH
                     LELDYGWMSVGGVLGWSGGTSIRRIQSFVEKPEDGDGKRLMSEGALWNTLVLVTKVET
                     LWKSGWLCFPEMMERFERLRRQIGTPAEGSTLQAIYQDMPIKNFSRHVLQPLASELGV
                     MELDDVTWSDWGRPERIVETLRSMGKKPAFPTEAFVGSGAPSMPQMTREMGYPYLSC"
     assembly_gap    1851..1950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2094..2561
                     /locus_tag="PKM72_08680"
     CDS             2094..2561
                     /locus_tag="PKM72_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNP60900.1"
                     /translation="MSKPGDAERIIWEGYPSWGQFTWLYFFSLWTGFRGFFLLRVGLP
                     GWEMWFVGAGLLLGVVVVLRYWAKYLLTSKRVVLRNGYSGKDMANVEFGRFKSVEVMQ
                     GPVARVFGIGTLVVHCEGSGRSVRFRGIKDPEVIETKLRALLPPSSPVLIQPS"
     gene            2606..3193
                     /locus_tag="PKM72_08685"
     CDS             2606..3193
                     /locus_tag="PKM72_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60901.1"
                     /translation="MIRSLSRRTALVLGLAVLTVAAALFLEFLLSVRADRPFGHTQVA
                     HLVGWTGLVFIGLTFVYPLKRWMYPNQVWSHGWFQVHIVFGIIGPLIIFVHAGVHFHA
                     LVPIIALVAMVLVVMSGITGQALHYLAFQTLYERRHELAFEGMNEGEIEAHLHGLALQ
                     EETLRWWKYLHGPLTWAFVSFTLLHIGGALFFGGF"
     gene            3197..3784
                     /locus_tag="PKM72_08690"
     CDS             3197..3784
                     /locus_tag="PKM72_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60902.1"
                     /translation="MGCPRPWVAPLIWVLTGLVIGALAWASPLYTEALWAPGNLSRYH
                     TDIARCLSCHEPFRGPTPQKCLVCHTWEAFQSRSEPDVRQLHRDAIQTRQACLVCHTE
                     HRGVLTAITIGRVENPHGELIFRVTGATSCSDCHVIQAGAGKPDGTLQRNAYVERLIQ
                     KGEGAHQLGHFAQCLKCHRGGQLDIEEEDEDEDDD"
     gene            3999..4421
                     /locus_tag="PKM72_08695"
     CDS             3999..4421
                     /locus_tag="PKM72_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60903.1"
                     /translation="MSSANSSVKPTGQPCTILVVDDDEAMRKLLVDTLQEKGCQVIES
                     ENGTEALNVLKSVVPNVIVTDLKMPGGGFPYLRLLQEVAPRCPIVVMTAYGDSQSKAK
                     ALECGVHGYFEKPLRIFDLKAWICQMCLVHPCGNLPFS"
     gene            4452..5876
                     /locus_tag="PKM72_08700"
     CDS             4452..5876
                     /locus_tag="PKM72_08700"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP60904.1"
                     /translation="MAGSFLNQDSNSDVSLSARIEGLTFLAHHLDEPVVIFNPRKELV
                     YANPAANKIAKDCPLIPSPSIPSSDSLVSNPQTPCDPCHAMELFQADMQVQDTFSCPS
                     PSPGATPGCPFPRAVPLKSGTGMTHFAILMGARGSETVMVAPPDQVSSDLSLQQHGDS
                     FPETLPLTIIGDSEPIQRLVEMVRLIAASEATVLIQGESGTGKELVAKIIHALSRRCL
                     RPFVVVECSALPETLLESELFGHVRGSFTGAVADRKGLFEEAEGGTIFLDEISDTTPA
                     FQARLLRVLQEGEIKPVGSNVSIKVNVRVISAGNKPLEGLVATKLFRADLYYRLAVLP
                     LTVPPLRERREDVHLLVKHFLVQSAIKHGRDPMDVSPDAQTALAQHPWPGNVRELENL
                     IERAVVTSRHSTIEVKDLFADHPLQPDKNDLSSIGKTARQEAERSRILQALRDAKGDK
                     TRAARTLSISRSSLYNKIRDYHIS"
     gene            6345..>10990
                     /locus_tag="PKM72_08705"
     CDS             6345..>10990
                     /locus_tag="PKM72_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNP60905.1"
                     /translation="MYHGKSRFLTASVFLALMGGGTFWASPSFVSAENASPPHAHQTG
                     MPSQSPGWAEQLKGQTIVEDAMEGRAERAALVEQQHQRMMQQMQKDMEHKGADTGAFD
                     SMSMIHQYGGGPENGLLASTVGAEPVSMNGGLCPKTAPVRKYDVAAINVEISLNQWLD
                     YYPGYMYALTENIDKIREEEAKNAAARETEGHADPGAVKNGIQDQWIQPLVIRGNQGD
                     CVKITLRNQLEFGEEVSLHINGSDMVMSQTGQPALTTNPDTVAEEGQAIEMEWYIHPD
                     TQEGGKQFHTFSNDRELTVMGLFGVFVVEPRGSKYYEPLGTGPATEATSGWQVMIDNG
                     DGPDFREFVLIYHEVGDEAFRPVNKHGDFLPQRDPLTDAYRPGARALNYRSEPFGINN
                     MHVQHEYFGFEDESMAYDAYTFGDAAPTIPRSYLGDPAKFRVVHGGSEVFHSHHPHGG
                     SIRWQRSPRATQMPVWNTGQNGPVKYPIVRTKSDRVDVEAIGPSEALDLETECGSGLC
                     QWLAGDFLFHCHVAHHYVAGMWGYWRVYNSMQEPGYQNDVMAPLRELPDRIGRIHKSV
                     TSDQLVGKTVSWFGKQFKIVDKGKSDWKAEPAVVNLKDWVEMQLTNQGKPGHKDDELG
                     QLKAYDASVLDWVWQGNKAMSEKEPTLGGNPKYRPEWLGYKPGERRAIAFEPKTGKVA
                     WPWLTPHFGKRAPFSNDHNPAPWLEMIRLNPDGTRSVEPAKAGENGPWSLCPDRAGSQ
                     DYKVHFIKLPIELSAAEGKEPAIVDPNGLLYVVHDEEEEVRANNDKKFPLVVRANVYD
                     CIDWTLTSEWLDDDITNFQSSKINTHFHFFQFDNQGSDGVSSGFSYEQSMRPFTEFEK
                     KTDKGLPIPMNAKVTQAAKKGDKTLVVSNAKQYHVGIPILIGADNVKGQEVQRIVKID
                     GSTLTFAQPLRNPHPVNDIVTVEYVRQRFWVDADVGSVFWHDHAFGGTTWPHGAVGTM
                     IAEPFGSTWHDPKTGKRIRTGPVADIHAVERVGHDVAGSFRELMVHIMDTVPHTVNIV
                     TAGNPPGQPVDVALEAGRTVSFIMPPNDKIKMTPMPFLNGGTHTTGGAFNFRAEPFAQ
                     RLVNNPDPSQIFSSVVHGDPSTAMVRAYLGDAIVFRLLDVTMNESNVFTLSGHTFWSE
                     RYAQEANRKHSLHIGIAERYDLVIPEAGGPRHQAGDYMFFNGRSSKFSEGSWGLIRVL
                     DKPVDDLQPLPNKAYGKEGMPERLPVCPKEAPVKSFNVVAINHPGMSFNSNAPESIEV
                     DFERKIELRNPDAKIYVLEDEVKKVGADAQPMPLTLRANVGDCLQVKLTNKLKEGRAS
                     FSAFGLAFDPKDSQGLNLGNNPGDQTVAPGESRMYTYYADPFNGETQTLVWDWGNVSM
                     NPRNGLFGGIIIGPKGSMYRDPKTGEDISLKNSWNADVIVDRTIQGLESRESYRDVAL
                     YFQDEDNIIGTSFMPYVQNVAGLTGVNYRAEPYLQREEAGCSLGRMFQPCQVDNPQDP
                     VTPVIEAHAGDKVRIHVFGASSEQNGMFTVEKHEWPIEPFLPGADMISTVEFSGS"
BASE COUNT         2550 a         2488 c         3099 g         2753 t
ORIGIN      
        1 tgcctaagaa cttttctcga cttctgaaaa ataagcatgg tagtgaggat atgctggttt
       61 catgccatag gttggtgcac ccaaggaatc cccgaatctg tttggttacc aatgctgaaa
      121 aaaaacaaaa gaatggtgct gaatagatgg cgatgttccc aggttgccca ggccgatgat
      181 gggggggatt ttgtttatga cgggctgagc gagttatctg gaaggttgga tgttcacgac
      241 gtttgcatta catcaatgtg cgtaaaacca ttccatgcct ccatctcgca tgaggaacag
      301 tttcctggaa attcttgttt acagaccatg aaggtcgaaa ccttcagcct gtttgattcc
      361 gcacattttt ggctgtgttg agtttggtga aaagatagaa aattcaacga attaaggatt
      421 cttctgactc tggcctttca attgcttacc ttttcattaa ttattcgaaa tgtaatatta
      481 acttttggct cctccgctat tatctgcaat ttaattagat tgctgtgtct ggaaaaacga
      541 gtgtgggttt tgctctatcg ggcatgttcc attagctacg ttttacaatt tcgaggagat
      601 ccatcatgac tgttcaaacc ctaccgtcct ccgtgtggtc catcgtgttg gcaggtggtg
      661 aaggcgaacg tatcaaaccc tcgatccagc agtggctcgg gtatccgatt ccaaaacaat
      721 actgcacctt tgtcggtact cgttctatgt tgcaacatac gtgggaccgg gccgaccaaa
      781 tcggtgtgcc ccggaagaaa gtaacggtgg tcgggcgaac tcatcagcag agtttggaac
      841 atcattgtac aagacaagac gagggcacat tgatctttca gccccgaaat tgtgatacgg
      901 cccccggcgt gtttcttcct cttacctacg tgaaagcctg ggatcctcaa gcagtcgtgg
      961 ttctcttgcc cgccgatcat tttatctgtc ccgaagatcg ctttgtggct gcggtgcgtc
     1021 gggccgtgcg cggggtagaa tttttatctg accgaatgat tctcatgggc gttcggccgt
     1081 cccatctgga gcttgattat gggtggatgt ccgtcggagg agtactcgga tggagtgggg
     1141 gaacttccat ccgtcggatt caatcgtttg tagagaaacc ggaggatggt gatggcaagc
     1201 gattgatgtc ggagggagcc ttgtggaaca ccctggtgct ggtgaccaaa gtcgagaccc
     1261 tgtggaaatc cggatggtta tgctttcctg aaatgatgga gcggtttgaa cggttgcgaa
     1321 ggcagatcgg gactccggcc gaaggatcga ctctccaggc tatttatcag gatatgccta
     1381 tcaaaaattt ttcacgtcat gtattgcaac ctcttgccag cgaattaggg gttatggagc
     1441 ttgatgatgt gacgtggagt gactggggcc gtccggaacg gattgtggag accttgcgat
     1501 caatggggaa aaagcctgct tttccaacgg aggcttttgt gggatcaggt gcgccatcca
     1561 tgcctcaaat gacaagggag atggggtatc cctatctaag ctgctgaagc aattatccct
     1621 ggctatcagg aagtgttttt tgctcgaagc cgtggtactc ggcgagcaag cattcaggta
     1681 gtgcgtccga tcaggacgtt ccagagctct gttccaaact gatctcacat accatcatag
     1741 gcccctttcc tccctggttg tatctccttc tgcggaaatc cttctcactg tttgtctcat
     1801 ttagacctat tggtctggtc gtggttggct atggtccttt ctgctatcca nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggcacggag gtcgcaagag agacctcatg
     1981 tgtggaatca tgattcagca cgttgtcgtg tttgtgggac taagatgtgt gtgttgagac
     2041 tttttttgaa gatgaaacaa tattccaggt ggtaaacaca aaggacgaca aacatgagta
     2101 agccaggaga tgccgaacgg attatctggg aaggctatcc ctcgtggggg caatttacgt
     2161 ggttgtattt ttttagtcta tggacgggat ttcgaggttt cttccttcta cgtgtagggc
     2221 ttcccggttg ggaaatgtgg tttgtcggag caggtctgtt gttgggggtg gtggtggtcc
     2281 tgcgatattg ggccaaatat ctgctcactt ccaagcgagt tgtgcttagg aatggatatt
     2341 cgggaaagga catggccaac gtggaattcg ggaggttcaa atcggttgaa gttatgcaag
     2401 ggccggtagc cagggttttt ggaatcggca cacttgtggt tcattgtgag ggttcaggca
     2461 ggagcgtgcg atttcgtggc atcaaggatc ctgaggtgat tgaaacaaaa ttacgggcgc
     2521 tcctccctcc ttcttctcct gtgttgattc agccatcctg aacgatgaat gaatctgctg
     2581 cgaggaggta agagaaatat cctttatgat ccggtctctt tcccgtcgta cagctttggt
     2641 gcttggtttg gctgttctga ccgtggccgc tgcccttttt ctggaattcc tcttgagtgt
     2701 cagagcagac aggcccttcg gtcacaccca agtggctcac ctggttggtt ggaccggatt
     2761 ggtattcatc gggctgacct ttgtgtatcc ccttaagcgg tggatgtatc caaatcaggt
     2821 ctggtctcac ggatggtttc aggtacacat tgtgtttggc attattggcc cgctgattat
     2881 ttttgtccat gccggtgtcc attttcacgc tctggtgccg atcatcgctc ttgttgccat
     2941 ggtcctggtg gtgatgagtg ggatcaccgg acaggctttg cattatctgg cgtttcaaac
     3001 attgtatgaa cgacggcatg agctggcttt tgaagggatg aatgagggtg agatcgaggc
     3061 gcatctgcat ggtctcgcgt tacaagaaga gacgctccga tggtggaaat atcttcacgg
     3121 ccccttaacg tgggcatttg tctctttcac gctgctgcac attggaggag cgctcttttt
     3181 cggcggcttc tgaatcatgg gatgtccccg accatgggtt gcgcccctta tctgggttct
     3241 gacaggtctg gttatcgggg ctctggcctg ggcttctcct ctctacactg aagccttgtg
     3301 ggcacctgga aatctcagcc ggtatcacac tgacattgcg agatgtctgt cctgtcatga
     3361 accgtttaga ggacccacac cgcaaaagtg tctggtctgt cacacctggg aagcatttca
     3421 atctcgatcc gaaccggatg ttcgccaatt gcatcgagat gccattcaaa cccgccaagc
     3481 ttgtctggtc tgccatacgg agcatcgggg tgtgttaacg gccataacga tcggtagggt
     3541 ggaaaatcct catggagaat taatttttcg ggtcacaggc gcgacatcct gctcggattg
     3601 ccacgtgata caagccgggg ccgggaaacc cgacggtact cttcaacgca atgcgtatgt
     3661 tgaacggttg attcagaaag gagagggcgc tcatcaactc gggcattttg ctcaatgtct
     3721 gaaatgccat cgtgggggac agttggacat tgaggaagag gacgaagacg aagacgatga
     3781 ttaggctgaa gttgccatca attttccata ctccacttta ttgggtgaca aatccggatt
     3841 tcgcttaggt tacatacaaa gatcctcaat caggtggaga aaaaatccac agcatcacct
     3901 cgtatgtcag gggcccggaa cttgctaggt aatatgtgta ttatgagcat tttcggttat
     3961 tcgaggggag tgccgggttg catcgggaga gtgtgaaaat gtcctcagcc aactcttcgg
     4021 taaaaccaac cgggcaacca tgcaccattc tggtcgtgga tgatgatgaa gcgatgcgga
     4081 agctgcttgt ggataccctt caagaaaaag ggtgtcaggt gattgagtcg gagaatggga
     4141 cagaggcctt gaatgtgttg aaatccgtcg tgccgaatgt gattgtgact gacttgaaaa
     4201 tgccaggagg gggatttccc tatttgcggc tgctccagga agtggctccc cggtgtccga
     4261 ttgtcgtgat gaccgcgtat ggagatagtc agtccaaagc gaaagctttg gaatgtggtg
     4321 tccatggata tttcgaaaaa cctcttcgaa tctttgattt gaaggcgtgg atttgtcaaa
     4381 tgtgtttggt gcatccctgc gggaaccttc ctttttcatg aggcagtgaa cgagaacaga
     4441 aagggtaaac gatggccgga tcgtttctga atcaggattc taattctgac gtgtccttga
     4501 gtgcgcggat tgaaggatta acgtttctgg cgcatcattt ggatgagccg gtcgtgattt
     4561 tcaatcctcg gaaggaatta gtgtatgcca atcccgcggc caataaaatt gcgaaagact
     4621 gcccattaat tccttcccca tccattcctt cttccgattc actcgtttcc aaccctcaaa
     4681 ctccctgtga cccctgtcac gcaatggaac tgtttcaggc tgatatgcaa gttcaggata
     4741 cattctcctg tccgtcccct tctcccggtg ccacgcctgg atgtccgttt ccacgcgcgg
     4801 tgccattgaa aagcgggacc ggcatgaccc attttgccat attgatgggg gctcgtggga
     4861 gtgagaccgt tatggtggct ccaccggacc aggtatcatc ggatctgtca ctacagcagc
     4921 atggggattc cttcccggag actttgcccc tgacgatcat aggagacagt gagccgattc
     4981 aacgattggt ggaaatggtt cgattgattg cggcgagtga ggccacggtg ctcatccaag
     5041 gagaaagtgg aaccggtaag gaattggtgg cgaaaatcat tcatgccttg agtcgtcgat
     5101 gcctccggcc ctttgttgtg gtggaatgta gcgcattgcc cgaaaccttg cttgaaagcg
     5161 aattattcgg ccatgtgcgt ggatccttta ccggagcggt tgccgatcga aaaggcttgt
     5221 ttgaagaagc cgaaggcggc acgatttttt tggatgaaat ttccgatacc actcctgcat
     5281 tccaagcccg tttacttcgc gttttgcagg aaggcgaaat caaacccgtg gggagtaatg
     5341 tatccattaa ggtgaatgtg agggtcatct ccgccggcaa taagcctctg gaaggtcttg
     5401 tggcaaccaa gttgtttcgt gccgatttat actatcgact cgccgtcctc ccgctaaccg
     5461 tgccgccgtt aagggagcgg agagaagatg tccacctgtt ggtgaagcat tttctggtgc
     5521 aatccgccat aaagcatggg cgtgatccga tggatgtcag tccggatgcc caaacggcac
     5581 tggcacagca tccgtggcct gggaatgtcc gggaattaga aaacttgatt gaacgggcgg
     5641 tggtcaccag ccggcattcc acaattgaag tgaaagactt gtttgccgat catccgttgc
     5701 aaccagacaa aaacgatttg agttcgatcg ggaaaactgc tcgtcaggaa gccgaacggt
     5761 caagaatcct ccaagccctc cgggatgcga agggagataa gacccgcgca gcccgcacct
     5821 tgagtattag tcgctccagc ctctacaaca aaatacggga ctatcacatt tcgtaatctg
     5881 ccacggtttt tcttgtcccc aaaatcccca ttttagtttt tttattgtcc atttttttgg
     5941 acgcacaact ggttgatttt ttgttgaaat ttttcgcgca ttgaaaagaa tgtcaaatgt
     6001 gttggacatt ctgccctgtt gggcgatttt ccatttcttc ctgcctatct cctgaagtcc
     6061 tctccagtac cgacttctgc ggtttgtagc atggatgagt gttcgttggc atcgatgttg
     6121 cggagtcaat gaattgccgg cgctggtttg ggtgtgatgg accggcctcc gaaatgcccg
     6181 acctctcaaa gaggaattct ggcgtcggca cttaacttaa tttggcactc aaagggtagg
     6241 aggtcgggct gttcttcgag aaatcggaaa tgttgaaacg agttggttgg ttgagagtaa
     6301 aaaaactgaa gttttattca ccccataagt aaggaggccg gagaatgtat cacggcaaga
     6361 gtagatttct cactgcgtcg gtatttttgg cgctgatggg aggggggacc ttctgggctt
     6421 ccccatcctt tgtcagtgcg gaaaatgcct ctcctccaca tgcccatcag acagggatgc
     6481 cctcacagtc tcctggttgg gcagaacagc tcaagggcca gaccattgtg gaagatgcga
     6541 tggaaggtcg agcggaacga gcggctctgg ttgagcagca gcatcaacgc atgatgcagc
     6601 aaatgcagaa ggacatggag cataagggtg ccgatacggg tgcgttcgat tccatgtcga
     6661 tgattcatca atatggagga ggccctgaaa acggcttgct ggcgagtacg gtcggtgccg
     6721 agccggtgtc catgaatggg gggctctgtc ccaagaccgc acctgtccgg aaatacgatg
     6781 tggcagcgat caacgtggaa atcagtttga atcaatggct ggattattat ccgggataca
     6841 tgtatgcctt gaccgaaaat attgacaaga ttcgtgagga agaagccaaa aatgccgcgg
     6901 cacgggaaac ggaagggcat gcggatccgg gagccgtcaa aaacgggatt caggatcagt
     6961 ggattcaacc gttggtgatt cgcggaaatc aaggcgattg cgtcaaaatc actctccgga
     7021 accagttgga atttggcgaa gaagtcagtt tgcacattaa tggttcggat atggttatga
     7081 gccaaaccgg tcaaccggcg ctcaccacga atcctgacac ggtggcggag gaaggccaag
     7141 ccattgaaat ggaatggtac atccatcccg atacgcaaga aggcggaaag caatttcata
     7201 ccttcagcaa tgatcgagag ctaacagtca tggggctgtt cggtgtcttt gtcgtagaac
     7261 cccgtggatc gaaatattat gaacccttag gcacgggacc agccaccgaa gccacgagtg
     7321 gatggcaggt gatgatcgat aatggggacg gtccggactt tcgggaattt gtcctgattt
     7381 atcatgaggt cggtgatgaa gctttccgtc ccgttaataa gcatggagat ttcctgccac
     7441 aacgggatcc ccttaccgat gcctaccgtc ctggtgcgcg ggcgctcaac tatcgcagtg
     7501 agccgtttgg catcaataac atgcacgtgc aacatgagta tttcgggttt gaagatgaat
     7561 cgatggcgta tgatgcctat accttcggcg atgcggctcc gaccattcca cgaagttatt
     7621 tgggagatcc tgctaaattc cgtgtggtgc atggcggatc ggaagtcttt cattcgcatc
     7681 atccccatgg tgggtcgatt cgatggcaac gcagcccacg ggcaacccaa atgcctgtct
     7741 ggaataccgg gcaaaacggg ccggtgaaat atcccatcgt ccgtacgaag tccgaccggg
     7801 ttgatgtgga agccattggg ccatcggaag cgttggatct ggaaaccgaa tgcggttcgg
     7861 gtctgtgtca atggttggcg ggagattttc tcttccattg ccatgtggcg catcactatg
     7921 tggctgggat gtggggctac tggcgcgtct ataacagcat gcaagagcct gggtatcaaa
     7981 atgacgtgat ggcgccgtta cgggaattgc cggatcggat cggccgaatc cacaagtcgg
     8041 taacatcgga tcaacttgtg ggcaagaccg tcagttggtt tggtaagcaa tttaaaattg
     8101 tggataaagg caagagcgat tggaaagccg agccggcggt cgtgaatctc aaggactggg
     8161 tagaaatgca gctcaccaat caaggcaagc ccggacacaa agatgatgag ctcgggcagt
     8221 tgaaagccta tgatgccagt gtcctggatt gggtgtggca aggcaataag gccatgagtg
     8281 aaaaagagcc gacgcttggt ggtaacccga aatatcggcc ggagtggctg gggtataaac
     8341 cgggcgaacg acgcgctatt gcgtttgagc ccaagaccgg aaaagtggct tggccatggc
     8401 tgacgcccca tttcggcaag cgcgcgccgt tttccaacga tcataatccg gccccctggt
     8461 tggaaatgat ccgattgaat ccggatggca cacggtcggt cgagccggct aaagccgggg
     8521 aaaacggccc gtggagtctg tgcccggatc gtgccggatc gcaagactat aaggtgcatt
     8581 tcattaagtt gccgattgaa ttatcggcgg cggaaggaaa agagccggcg attgtggacc
     8641 ccaacgggtt gctgtatgtc gttcacgacg aagaagaaga ggttcgagcc aacaacgaca
     8701 aaaaatttcc gttggtggtg cgagccaatg tgtatgattg catcgattgg acgctgacca
     8761 gtgagtggtt ggatgacgat atcaccaact tccaatcgtc taagatcaat acgcatttcc
     8821 atttcttcca gtttgacaat caaggttcag atggcgtgag ttcgggcttt tcgtacgagc
     8881 agtccatgcg cccgtttact gaatttgaaa aaaagacaga taaagggttg ccgattccga
     8941 tgaatgccaa ggtcacccag gccgccaaaa aaggcgacaa aaccttggtt gtttccaatg
     9001 ccaagcaata tcatgtgggc attccgatct tgattggtgc ggataatgtc aaaggccagg
     9061 aagtgcagcg gatcgtcaaa attgatggca gtacgttgac gtttgctcag ccattaagga
     9121 atccgcatcc ggtcaatgac attgtgaccg tggaatatgt ccggcagcgg ttctgggtgg
     9181 atgcggatgt cggcagtgtg ttctggcatg accatgcctt tggcggcacg acgtggccac
     9241 acggggcagt cggaaccatg attgccgaac catttggttc cacctggcac gatccgaaga
     9301 ccgggaaacg gattcgaacg gggccggtgg ccgatattca tgcggtggaa cgtgtggggc
     9361 atgatgtggc cggaagcttc cgtgaattga tggtgcacat catggatacg gtgccgcaca
     9421 cggtgaacat cgtgacggcg ggcaatcctc cagggcaacc ggtggatgta gctttggaag
     9481 ccggtcggac ggtctcgttc atcatgccgc cgaatgataa aatcaagatg acgcctatgc
     9541 cgttcttgaa tggcggtacc catactactg gcggggcatt caatttccgg gcagagccgt
     9601 tcgcacaacg gttggtcaat aacccggacc catcgcaaat tttcagcagt gtggtacatg
     9661 gcgatcccag cacggccatg gttcgggcct acttgggcga tgcaattgta ttccgcctgt
     9721 tggatgtcac gatgaatgaa agtaatgtgt tcacgttatc tgggcatacc ttctggtcgg
     9781 aacgatatgc gcaagaagcg aatcgcaagc attcgttgca tatcgggatt gccgaacggt
     9841 acgacttagt cattccggaa gcaggtggtc cgcgccatca agccggcgat tatatgttct
     9901 ttaacggccg aagttcgaag ttttcggaag gttcttgggg gctcatccgg gtcttggata
     9961 aaccggtgga tgatcttcag ccattgccga ataaagcgta tggcaaagaa ggcatgccgg
    10021 agcgattgcc ggtgtgtccc aaagaggctc ctgtgaagag cttcaatgtt gtggccataa
    10081 atcatccggg gatgagtttt aatagcaatg caccggaaag cattgaagtc gattttgaac
    10141 gcaagattga attgcgcaat cctgacgcca agatctatgt cctcgaagat gaggtgaaaa
    10201 aggtcggggc cgatgcgcag cccatgccgt tgacattacg ggccaacgtg ggtgactgtc
    10261 ttcaagtcaa attgacgaat aaactcaaag aagggcgagc gtcgttctcg gccttcggac
    10321 tggcatttga tcccaaagat tcccaaggtc tgaatttggg gaacaacccc ggcgaccaaa
    10381 ccgtggcgcc tggggaatcc cggatgtata cctattatgc ggatcccttc aatggtgaga
    10441 cgcagactct ggtgtgggat tgggggaatg tatccatgaa tccccgcaac ggcctgttcg
    10501 gcggaatcat tatcggtccc aaaggatcaa tgtaccgtga tcccaaaacc ggggaagaca
    10561 tttccctgaa aaatagttgg aatgccgatg tgatcgttga tcggaccatc caagggcttg
    10621 aaagccggga gagctatcgg gatgttgcct tatattttca ggacgaggat aacatcatcg
    10681 ggacgagctt catgccctat gtgcaaaatg tggcggggct gaccggcgtc aactatcgcg
    10741 ccgaacccta tttgcagcgg gaagaagccg gttgctcgtt gggccggatg ttccaaccgt
    10801 gccaagtgga caacccgcag gatcctgtga cgccggtgat tgaggcccat gccggtgaca
    10861 aagtgcggat tcacgtcttt ggcgccagca gtgagcagaa cggaatgttc actgtggaga
    10921 agcatgaatg gccgattgaa cccttcctgc ccggtgcgga tatgattagc accgtggagt
    10981 tctcgggttc
//
LOCUS       DAORAQ010000084        10784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_992_length_10784_cov_62.302545, whole genome shotgun sequence.
ACCESSION   DAORAQ010000084 DAORAQ010000000
VERSION     DAORAQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_992_length_10784_cov_62.302545"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..719)
                     /locus_tag="PKM72_08710"
     CDS             complement(156..719)
                     /locus_tag="PKM72_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNP60906.1"
                     /translation="MNDVKPRGLRAGSLQWPDMHSGEFSCRVSLLLKLAFLGAGVCFV
                     YWVGWPQLALSPGVPLENSGVLESRTFLSGSADHPAEVVNVATHSGRDVVYPPQVMVK
                     GEPGPTREIAGVTVDLNDGTLAELEHLPGIGPVLAGRIMAHRTSHGAFRHIEDLVLVP
                     GIGEKRLELLRPFVGVGPSTKAIGIGS"
     gene            complement(751..2151)
                     /gene="lpdA"
                     /locus_tag="PKM72_08715"
     CDS             complement(751..2151)
                     /gene="lpdA"
                     /locus_tag="PKM72_08715"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNP60907.1"
                     /translation="MTISPHIVIIGSGPGGYVAAIRAAQLGAMVTILEEQVLGGVCLN
                     WGCIPSKTLLGFIDLGERVRHAQPFGLNIEGPVTYDLARMVARKEEVVRGLVKGIGTL
                     LKQWKITHVPGRGELVSNRQVRITHPDGSSSTLDADAIILATGSSWPQLPQFPIDGQR
                     VLTSKDALDLTEIPRSMVIVGGGVEGCEFACLLSGLGSEVSVVEMMPSVLPLEDEDTI
                     STMTRELKKRKIQLYLNTQISKWTAVDEGVRVTLASGEELVAGHLLVSVGRRLNSGQL
                     GLDQVGVEIGSKGEILVNEKMETSVPGIYAIGDVTGKFMLAHVASAQGKVAVSNAMGR
                     PQAINYDVIPAGIFTLPEIGRVGLTEAQARERGLAVAIGRFKYAGLGKAQAVGEPFGH
                     FKVISDEQTRKILGVHIIGAHAADLIHEAAMAMQGGLTVEDLANMIHAHPTFSEGLME
                     AAEDVEGMAIHQVRKR"
     gene            complement(2148..2534)
                     /gene="gcvH"
                     /locus_tag="PKM72_08720"
     CDS             complement(2148..2534)
                     /gene="gcvH"
                     /locus_tag="PKM72_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNP60908.1"
                     /translation="MEPQDLRFHKEHEWIRVEGNKATLGISQFAQDALGDVVFVDVPK
                     VGTSLQAEDQLGEVESTKATSTIYTPVTGKILEVNAELQDHPELLNQDPYGKGWIAVL
                     ELANPGEVDALMTPEQYTAFLASQES"
     gene            complement(2645..4198)
                     /locus_tag="PKM72_08725"
     CDS             complement(2645..4198)
                     /locus_tag="PKM72_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60909.1"
                     /translation="MRTWLTICSLALCLGLLAGCGRTRPPQGPPVSGAIPPSAVESVP
                     DRDEGGAPSLPGVSPDIQRIHSQLFQQSKAYFEEKAYPQTISGLTRLLALLPEESIEV
                     QSRWLLAQSYRQVGEWESARDQYRTLAVAGSTNPYQADAQLQFSELQRLLEQFAMPPL
                     DTQAIRLTLEQLPASGGFDQGIKKMKADGITTLLIDLGCEGAGFHTDQSRTSKAPRHL
                     FDLQALLRSYVERSHQVHLLVYVGVNLRCVGNWHDSPLPEWRDRTYNVTTRQAQESRF
                     FDLFHPEYQRFLDDFLAQLSGEGVDGLVFLDDYPMGMYDGISRSGLTRFQTAFGVSFD
                     PVRAFQAGFDPLKASKVSSRSTQGTNAFEEDSAFWRWAGWKARERLTVVEQLMGQLRK
                     RHLTVQCGLELHPHGLTDPVRALVDYAQDAMEAIRRPFSFFFVRPEIDRHSSSDQLSA
                     IEKLRRISTKAVLTRLLPVLDDPRRVWVSMPAERGKRIAPGTAGGSASLLDEFPSGIG
                     IVHDIRAFS"
     gene            complement(4343..4759)
                     /gene="ndk"
                     /locus_tag="PKM72_08730"
     CDS             complement(4343..4759)
                     /gene="ndk"
                     /locus_tag="PKM72_08730"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNP60910.1"
                     /translation="MSERTLAIIKPDAVAKHAIGDIVRRYEEAGLFPVAMKMMRLSQG
                     MAEGFYAVHRERPFFKDLTTYMSSGPVVVVVLEGKDAVKKHRDLMGATDPKKADPGTI
                     RAAHGASIEANAVHGSDSAENALIEIRYFFAESNLG"
     gene            complement(4860..6155)
                     /locus_tag="PKM72_08735"
     CDS             complement(4860..6155)
                     /locus_tag="PKM72_08735"
                     /inference="COORDINATES: protein motif:HMM:NF026052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60911.1"
                     /translation="MQPSHSVPLVYNLFPRLAGPCGRWVAHAERAASMGFNWLFVNPI
                     HLPGFSGSLYAIKEYDLLNPVFLPEGTQDQRLDVLRPTLQDIAQCGLAPMVDLVLNHT
                     AIDSSLVSQHPDWYMRDEHGKVENPYAVDPNDPSKKTVWGDLAEIDNRHSPDREHLWE
                     FWGQLVDRYLGLGFQGFRCDAAYKVPGELWSFLIERAQRVNPQAVFWAENLGCTLEQT
                     RALGDAGFHFFCNSSKWWNFKDAWCLTQHREFQNMPSIGFPESHDTPRLAAESGGNEA
                     VQRQRYAFAALFSTGLMIPMGYEFGFQKSLHVVETTPDDWESPRWDLQDFIRAVNHWK
                     LEVPLWQGEGELVQRSMSHSNLCVLERRSLDNSHSWALLCLNTNLYEPVDVAIGELGT
                     LPSSPRMWRVSSLDQHPGSHSIPERVVFQPAEVIVITDF"
     gene            complement(6166..7524)
                     /locus_tag="PKM72_08740"
     CDS             complement(6166..7524)
                     /locus_tag="PKM72_08740"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNP60912.1"
                     /translation="MKEILFVGAGSVGGYFGAHLSRHNPNVSFLLRPRSREAVAKNGL
                     TIRSAIGESFTVHPKVVSDPKELPQPDLIVLGIKAYDLDEVMDQIEPVLKPDTTILTL
                     QNGVTIEDTLMARFGRERIVGGVAFIYSRIAEPGVIDHYKKGMLAIGEPLGMETPRLQ
                     VILELFKGGGLPCFINPDIRRAKWEKMCWNCVFNPLTVLLNDRVAKALDHPEFQPVIA
                     TIVREVSAVAMAAHRVPLDEDMPQKVVKWAQELRDIHTSMYDDWLAGRPTEIDELNGF
                     ISEQGKAFAVPTPMNDMLTAFIKAMTLSKPSVEATIEVGGAIMQSLQLTRTTLAQLPA
                     AHQISELPALMPGMNIRGVKLQAILDVVTLDLGADHVTFESQDGKFSACLTIKQAVEF
                     GILVYEMDGAPFPTERGGPFRLVTPGLGDLCANVKQVGSMVVSKGLVSDSRPPQVCHE
                     KE"
     gene            complement(7769..8233)
                     /gene="trmL"
                     /locus_tag="PKM72_08745"
     CDS             complement(7769..8233)
                     /gene="trmL"
                     /locus_tag="PKM72_08745"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNP60913.1"
                     /translation="MLDVVLYEPEIPPNTGNIIRLCANTGFGLHLIHPLGFELDDKRA
                     RRAGLDYHELARMKDYPTLQDYLDAHKPSRIFAITTKGQRPYHDVAFQPGDALYFGPE
                     TRGLPAHILEALPPDHRLRIPMRPDSRSLNLSNAVAVVVYEAWRQLQFYGAK"
     gene            complement(8680..>10784)
                     /locus_tag="PKM72_08750"
     CDS             complement(8680..>10784)
                     /locus_tag="PKM72_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60914.1"
                     /translation="PQLQTVTGTDLINQGLTGGDYQETGPANTAAQAINSAGNWMNQN
                     PTDSTGQGAIDLSQIKTPHWEIDFAHPEGPRFRVLTEEEARDLLRNRRATGQLGSSGG
                     IFGDVAHFFKHEFDQLTYFAATVTDDVLNIVFNDLEPFVISTIKQAGAALETIFSKIR
                     QLADEIYKVLQEVIAWLKMLFDWDDILNTHSVFKECMNQTLTNLSGSITYAQTLVQKN
                     FAEVQAEVKEIFTNLESKFEPIQGQSYNDFANSASESQNTLADPSTQSTQQQHASKCN
                     YVYSRSTPSFTGASAISLRDLEGTLGDSDPTTVITDAVKQYIYSGNTSDQNTQQFNST
                     NATLQNFVTGKITNPSSLFDLIIVDLLTAAEDLVLFIMDGIEYVIEAILSVAASAIKA
                     FSDLINQPIDIPIVTWLWKNVITKGDEFTLLDLVCLIFAVPATILYKLVASTLLNPNE
                     APQAPFSSSDVTTIQTTGLPWPTIPSEGVPTMAARTSDSSQLSAKIMASLTATSSFFM
                     GLGYLMTMVSDYKAYQSQQEGAFVPSPKATFYSYLSLFFRSMTFATGAPGSVFAKPSS
                     QWAIADRWTVGLWSTRGALVLADLVYCALSVYKTGAKWVPVYGPALKSAMGVLHIGIG
                     AGASYYQLSDNNYSDWVPINNFLRPIPSCFKFLFYDPPESGVWAPIPWLLVIDAVAGV
                     GATATQIGAAVYKFQHSN"
BASE COUNT         2669 a         3059 c         2667 g         2389 t
ORIGIN      
        1 tcttgagagg acgctgttct ttcaaggact ctgctaattt ggcttttaat tcgtcgagct
       61 gaatgacctc cacggttccc cgaagaatca gatccagttg ccggttaatt tctgaagttg
      121 tcattttata agccacgctg tgtgaaagtg aaaaattaac tcccgatacc tatcgccttg
      181 gttgagggtc ctacaccgac aaaggggcgc agtagctcca accgtttttc tccaataccc
      241 ggcacaagta ccaggtcctc aatgtgtcga aatgctccat gggaagttcg gtgcgccata
      301 attcgtccgg caaggaccgg tccaatgcca ggaagatgct ctaattcagc taaggtgcca
      361 tcgttcaaat ccacggtcac ccccgcaatt tcacgggttg gtcccggttc ccctttcacc
      421 atcacttgtg gagggtaaac gacatctctc cccgaatgag tcgccacgtt cacaacttcg
      481 gcgggatgat cagcggaacc ggaaaggaat gtgcgtgatt cgagaacacc tgaattttcg
      541 aggggaacgc ctggagaaag cgctagctgc ggccacccga cccaatagac gaaacacacg
      601 cctgccccca aaaaggccaa tttcaataga agagagactc tgcaggaaaa ttcgcctgaa
      661 tgcatatcag gccattgcaa cgacccggct cgcaatcccc ttggtttaac gtcattcatg
      721 ctgcatcaat gaccacatct cacggcatta ttaccgcttt cgcacctgat gaatcgccat
      781 gccctccaca tcctcagccg cctccatcaa cccctccgaa aatgtcggat gagcatggat
      841 catgttggca agatcctcga cggtcaaccc accctgcatc gccatagcgg cttcatgaat
      901 aaggtccgcc gcatgggcac cgataatatg gacgccaagg attttcctgg tctgttcatc
      961 cgaaatcact ttgaaatgtc caaatggctc cccgacagcc tgggcttttc cgagaccggc
     1021 atatttgaat cgcccgatcg cgacggccaa accacgttcg cgggcttggg cctcggtcag
     1081 cccgactcgc ccgatttccg gcaaggtaaa tattcccgca ggaatgacat cataattaat
     1141 ggcctgcggc cggcccatcg cgtttgagac cgccaccttt ccttgagccg acgcaacatg
     1201 agccaacatg aattttcccg tcacatctcc aatcgcataa ataccgggaa cggaggtttc
     1261 cattttttcg ttgaccagaa tttcaccctt gcttccgatc tcaaccccaa cctgatccag
     1321 ccctaattgg ccggagttca aacgacgccc gaccgaaacc agcaggtggc cggcaaccag
     1381 ctcttctccc gaagccagcg tcactcgcac gccttcatca actgcggtcc atttggaaat
     1441 ctgcgtattg agataaagtt gaatcttgcg tttcttcaat tcacgggtca tggtggagat
     1501 ggtatcttca tcctcgagag gcagcaccga aggcatcatt tccacgacgc tcacttccga
     1561 tcccagcccg ctcaacaggc aggcgaactc acagccttct accccgccgc cgacaatcac
     1621 catgctcctg gggatctccg ttaaatccaa cgcgtcttta ctggtcagca cgcgttgacc
     1681 atcaatagga aactgaggca gttgaggcca ggaagaaccg gtggcaagaa taatggcatc
     1741 ggcatcaagg gtggaggatg acccatcagg gtgggtaatc cgtacctgcc gattactgac
     1801 taattcgccc cgccccggca cgtgcgtaat tttccactgc ttcaataacg tgccaatccc
     1861 cttcaccaac ccgcgaacca cttcctcctt gcgcgcgacc atgcgggcaa gatcataggt
     1921 cacaggccct tcaatgttga ggccaaacgg ctgggcatgc cgaacccgtt caccgagatc
     1981 aataaagccc aacagggttt tactgggaat acagccccaa ttcaggcaca cccccccaag
     2041 cacctgctct tccaggatcg tcaccatggc ccctaattgg gcggcacgaa tagccgccac
     2101 atacccacca ggacctgatc cgataatgac aatgtggggc gagatggtca tgattcctgt
     2161 gaggctaaaa atgccgtgta ttgctccggc gtcatcaacg catccacttc accggggttg
     2221 gctaattcca gcaccgcgat ccaccccttt ccatagggat cctgattgag cagttcggga
     2281 tggtcttgaa gctctgcgtt gacttcaaga atttttccgg tcaccggcgt gtaaatcgtc
     2341 gaggtggcct tggtcgactc cacttcgccc aactgatctt cagcctgcaa tgaggttcct
     2401 actttaggaa cgtccacaaa gaccacatcc cccagggcat cctgagcaaa ttgactgatg
     2461 cctaacgtcg ccttgttccc ttcaacgcga atccattcgt gctccttgtg aaaacgtaaa
     2521 tcctgtggct ccattacaac ccccttttgt tcaaaacgat gatggcgttc atatgtgtgt
     2581 gcctcccatg cacgaggttg agtgggtgca cccacctcca tgggccagag acatcagaga
     2641 aaaatcaaga aaaggcgcga atatcgtgta cgataccaat tcccgaagga aactcatcaa
     2701 gaagtgacgc gctgcccccg gctgttcccg gcgcaatgcg ctttcctctc tccgccggca
     2761 tactcaccca caccctccgc ggatcatcaa gcacaggaag caagcgggtc aacacagcct
     2821 ttgttgagat gcgccggagc ttctcaatcg ccgacagttg atcggacgat gaatgacgat
     2881 caatttccgg tcgcacaaag aaaaaggaaa acgggcgccg gatggcttcc atggcatcct
     2941 gggcatagtc cactaaagcc cgaaccggat cggtcaatcc atgcgggtgg agttccagac
     3001 cacattgaac cgtcaaatgg cgcttgcgaa gttggcccat caactgttca acaacggtca
     3061 atcgctcgcg agccttccac ccggcccatc gccaaaacgc ggaatcttct tcaaacgcgt
     3121 ttgtaccctg tgttgatcga ctcgacacct tcgatgcttt gagaggatca aaccctgctt
     3181 ggaaggcacg cacgggatca aagctcacac cgaatgcagt ttgaaaacgg gttaaccccg
     3241 agcgggatat cccgtcatac atccccatgg gataatcgtc gagaaacacc agaccgtcta
     3301 caccctcccc ggaaagttgt gccagaaagt catcgagaaa ccgttgatac tctggatgaa
     3361 acagatcaaa aaacctggat tcctgagcct gacgggtggt cacattatag gtgcggtcgc
     3421 gccattccgg caaaggagaa tcgtgccaat tgccgacaca acgcagattc actcccacgt
     3481 acaccaacag atgcacctga tgacttcgtt ccacatatga gcgcaagagc gcttgtagat
     3541 caaacagatg ccgaggcgcc ttcgacgtcc gcgattgatc ggtgtggaat ccggctccct
     3601 cacaacccaa atcaatcaac agggtggtga ttccatcggc tttcatcttt ttaattccct
     3661 ggtcaaaccc tccactggcc ggcaattgtt caagagttaa ccgtatggcc tgagtatcga
     3721 gaggaggcat cgcaaattgt tcaagtaacc gttgaagttc gctgaattgc aactgagcat
     3781 cagcctgata gggattggta ctccccgcaa cggcaagcgt gcggtattga tctctggcgg
     3841 actcccattc ccctacctga cgataggatt gagccagcaa ccaacggctt tggacttcaa
     3901 tcgattcttc aggaagaagc gccagcagcc gcgtgagccc gctgatggtt tgaggatagg
     3961 ctttctcctc aaaatatgct ttcgattgtt gaaagagttg tgaatggatt cgttgaatat
     4021 ccggagagac tccgggtagg gatggcgcgc ctccttcatc acgatccggt acggattcga
     4081 cagcagaagg aggaatggca ccactcaccg gtggtccctg tggaggacgt gtccgtccgc
     4141 acccggccaa gagccccaga cacagggcca gactacaaat tgttagccag gttctcatac
     4201 ggcattaggt gtatacgtgc agagggacat gagcccaaaa ccaatacact atatctataa
     4261 gaagagacag gtatgcccgc cgatgtcggc gggcaccatg ccaaggtcat tgagcaccaa
     4321 ccaatttagt ttggtttttt cactagccta gattggattc cgcgaaaaag taccgaatct
     4381 caattagagc attttccgca gaatcggacc catgcaccgc attggcctca atagaagcgc
     4441 catgcgcagc ccgaatggtg ccaggatcgg ctttcttggg atcggttgcc cccatcagat
     4501 cacggtgttt tttgacggca tcctttcctt ccaacaccac gaccaccact ggcccagagc
     4561 tcatataggt ggtcaagtct ttaaaaaatg ggcgttctcg atggacagca tagaaccctt
     4621 ctgccatccc ttgggataac cgcatcattt tcatagcaac cggaaataac ccggcttcct
     4681 cataccggcg gacaatatca ccaatcgcat gcttggccac cgcatcaggt ttaataattg
     4741 ctaacgtccg ctctgacata ccttctcgtc ctttcccaaa aatcccataa aaaaaccctg
     4801 tgaataagaa aggagcattt tacggcccct tctattcagt tgcaatctca agaccacact
     4861 taaaagtctg taatcacaat gacttcggct ggctgaaaca cgacccgctc agggatggaa
     4921 tgggaaccag gatgctgatc gagcgagctg acacgccaca ttctgggaga agacggtaga
     4981 gtgcccaact caccgatagc tacatcaacc ggttcataga gattcgtatt gaggcagagg
     5041 agtgcccaac tgtgggaatt gtccaacgac cgccgctcca aaacgcacag attggagtgg
     5101 ctcatcgacc gttgaaccag ctccccctcc ccctgccaaa gcggcacttc taatttccag
     5161 tgatttacgg cacggataaa atcctgcaaa tcccatcggg gggattccca atcatcgggg
     5221 gtggtctcca ccacatgcaa cgacttctga aatccgaatt cataccccat cgggatcatg
     5281 aggccggtgg aaaacagcgc ggcaaaggca tagcgttgcc gttggaccgc ctcattccct
     5341 ccagactcag ccgccaggcg cggggtgtca tgggactcgg gaaatccaat ggacggcata
     5401 ttctgaaatt cacggtgctg ggttaaacac caggcatcct tgaagttcca ccatttggag
     5461 ctgttacaaa aaaagtgaaa ccccgcgtct ccaagcgccc gcgtctgctc cagagtacag
     5521 cccagatttt ccgcccaaaa gacggcctgc ggattcaccc gttgcgcccg ttcaatcagg
     5581 aaactccaca actcacctgg aactttatag gccgcatcac atcgaaaacc ctgaaacccc
     5641 aacccaagat accggtcgac caattgcccc caaaactccc agagatgctc acggtccggc
     5701 gaatgccgat tgtcaatttc ggctaagtcg ccccacaccg ttttcttgct gggatcattc
     5761 ggatcaaccg cataagggtt ttcaactttt ccgtgttcat cccgcatata ccagtcgggg
     5821 tgttgggaca ctaaagagga atcgatagca gtgtggttta agaccagatc taccataggc
     5881 gccaggccac attgcgcgat atcttgaagc gttggccgca acacatctag acgctgatcc
     5941 tgagtccctt ccggtaaaaa taccggattc aagagatcgt attccttgat cgcatataaa
     6001 ctccccgaaa atcctgggag atgaatggga ttcacaaaga gccagttaaa tcccatgctt
     6061 gcggctcgtt ccgcatgagc aacccagcga ccacaaggac cggccaggcg tggaaacaga
     6121 ttatagacaa ggggaacact gtgcgaaggc tgcatcaaga cacacctact ctttctcgtg
     6181 acagacttga ggtggacgac tatcagaaac caggcctttg gaaacgacca tgctgccgac
     6241 ctgtttcaca ttggcgcaca agtcacccaa gccaggggta actagccgga acggcccgcc
     6301 gcgttcggtg ggaaagggcg cgccatccat ttcatacacc aggatgccaa actccacggc
     6361 ttgctttatc gtcagacagg ccgaaaattt tccatcctgc gactcaaagg tcacatggtc
     6421 ggctcccaga tcaagcgtca caacatcaag tatcgcttga agtttaacgc cacgaatatt
     6481 catgccgggc atcaatgcgg gcaattcaga aatctgatgc gccgcaggaa gttgggccag
     6541 cgtcgttcgc gtcaactgga gagactgcat gatggccccg cctacctcta tcgtagcctc
     6601 cacagaaggc ttggataaag tcattgcctt aatgaaggcc gttaacatgt cattcatggg
     6661 agtgggcacg gcaaatgctt tgccttgctc gctaataaaa ccgttcagct catcaatctc
     6721 agtgggccgt ccggccaacc aatcatcata catcgaggta tggatatcac gaagttcttg
     6781 agcccattta acgaccttct gcggcatatc ctcgtccaac ggcacacggt gcgccgccat
     6841 ggccacggcc gagacctccc gcacgatggt cgcgatgacc ggttgaaact ccggatgatc
     6901 cagggctttc gctacccggt cattcagcaa taccgtcaaa gggttaaaca cacaattcca
     6961 acacattttc tcccatttcg cgcgccggat atcaggattg atgaaacagg gaagtcctcc
     7021 acctttaaat aattccagta tgacttgaag tcttggggtc tccatcccca acggttcgcc
     7081 aatggccagc atcccttttt tataatggtc aatgactccg ggctcggcga tcctggaata
     7141 gataaaggcc accccgccca caatccgctc acggccaaac cgggccatca gggtatcctc
     7201 aatcgtcacc ccgttttgca acgtcagaat ggtggtatcc ggtttcagca ccggctctat
     7261 ctgatccatt acctcatcca ggtcataggc tttaatcccc aacacaatca gatccggttg
     7321 gggcagttcc ttgggatccg aaaccacctt gggatgcacg gtaaagctct caccaatcgc
     7381 gctccggatg gtcagcccgt ttttagcaac tgcctccctg gaacgcgggc gcaggagaaa
     7441 cgacacgttc ggattgtggc gagacagatg cgcaccgaaa tatcccccaa ccgaacccgc
     7501 ccccacaaac aaaatctcct tcatccgatg acctttctta atagataaaa attcgtgtga
     7561 tactttagat atctggcggc cagtttaagc atttatgaga cctcaacaac atggtcatcc
     7621 tgagttggaa gcgaagggtc tgtgcctagg acaaacagtc ctagaaccca gcaacattct
     7681 tcgattcagg attcccccga gaaatgccaa aaaaaatgaa aggccctact ctagcagaat
     7741 tagcctcact cgcctacgga cctttggctt acttggcgcc ataaaactgc aactggcgcc
     7801 acgcctcata gaccaccact gccaccgcat tgctcaaatt caggctacga gagtctggcc
     7861 gcatgggaat gcgcaaccga tggtcgggag gaagggcttc caggatatgg gcaggcaggc
     7921 cacgcgtctc ggggccaaag tacaaagcat caccaggttg gaaggccacg tcatgatagg
     7981 ggcgctgtcc ttttgtagtg atcgcaaaaa ttcgtgaggg cttatgagca tccaggtagt
     8041 cctgcagcgt gggataatcc ttcatcctgg ccaattcatg ataatccaac ccggcccgac
     8101 gcgcccgctt atcatccaac tcaaatccca gcggatgaat cagatgcaaa ccgaatccgg
     8161 tattggcaca taaccgaata atattcccgg tgttcggtgg aatttcgggt tcgtagagga
     8221 caacatccag catcgtagga attaaggaac cggatatgag acttccatga atactttcaa
     8281 ataagacaga ccgtcagtcg aacagccttc actgttcaat gctgccattt ttgaggaaag
     8341 ccgtggtatg gggggcgggg ctatgaaaaa ctggcaggaa aagagaggct tcgctttcca
     8401 tttccagtac gcatagcttg cgtttaaaat ggtccgtctc tgcaaaacag ctcttcagct
     8461 acccaagagg gcggagttct cctaacacct cgcagcacaa aatgtgttcc tcacaaccgc
     8521 gcacaccggc ccctagtgtc acttcgattg gtaacacatt tccagtcccg ttggtaaggt
     8581 ggccttcaat ttcaagttca gaaagtaggg agcagaatca tttccaacct cttcaaagcc
     8641 aggaacctat caaggcaatc actcctgatt ttctgtaccc tagttcgaat gctggaattt
     8701 atacaccgcg gcgccgatct gagtggcggt cgctccgacg ccggcgacgg catcaatgac
     8761 gagtaaccat ggaatcggag cccacacccc tgattccggt ggatcgtaga acaagaattt
     8821 gaaacagctg gggatcggtc ggagaaagtt gttaatgggc acccaatcgc tatagttatt
     8881 gtcgctcagc tgatagtagc tggccccagc cccaatacca atatgaagca cgcccatagc
     8941 ggatttgagc gccggcccgt agaccgggac ccactttgcc cctgtcttgt agacggacaa
     9001 ggcacaatac acgagatccg ccaggaccaa cgctccccgt gtgctccaca gccccacggt
     9061 ccatcgatcg gctatggccc actgggagga gggtttcgca aacacgctgc ccggcgcacc
     9121 ggttgcaaat gtcattgagc gaaagaatag gctcaagtag gagtaaaacg tggccttggg
     9181 tgaagggaca aaagcgcctt cttgttggct ttggtaggct ttgtaatcgc tcaccatggt
     9241 catgaggtag ccaaggccca taaaaaacga agaggtggcg gtcagactgg ccataatctt
     9301 tgcagacaat tgagagctgt ccgacgttcg cgctgccatt gtcggcacac cctcggaggg
     9361 tatggtgggc caagggagac cggtggtctg gatcgtggtg acgtcgctgc tggaaaacgg
     9421 agcttggggt gcttcgttcg gatttaatag cgtgctggcg acaagcttgt agagaatggt
     9481 cgccggtacg gcaaagatga gacagaccag gtcgagcaag gtgaactcgt cgcccttcgt
     9541 gatcacgttc ttccacagcc aggtgacgat cggaatgtcg atgggctggt tgatcaggtc
     9601 ggaaaacgct ttaatggcgg acgccgccac cgagagaatc gcttcgatga cgtactcgat
     9661 gccgtccatg ataaacagca ccagatcttc cgccgcggtc agcaagtcca cgatgatgag
     9721 gtcgaatagg ctgctgggat tagtgatctt cccggtaaca aaattctgca gcgtggcatt
     9781 ggtcgaattg aactgctgcg tgttttggtc cgaagtattg ccgctataga tgtattgctt
     9841 caccgcatcc gtgatgacgg tcgtggggtc gctgtcgccc aaggtgcctt ccaggtccct
     9901 cagcgagatg gccgatgccc cggtaaacga gggtgtcgaa cgactgtaga cgtagttaca
     9961 tttcgaggca tgttgctgct gggtcgattg ggtgctcggg tcggccaagg tgttttggct
    10021 ttccgaagcg ctattcgcga agtcgttata gctctgccct tggatcggtt cgaatttcga
    10081 ctcgagattg gtgaagattt cctttacttc tgcctgcacc tccgcaaagt ttttctggac
    10141 gagtgtttgc gcataagtga tggagccact taggtttgtc agagtctgat tcatacattc
    10201 tttaaacacg gaatgcgtat tgagaatatc gtcccaatcg aagagcatct tcaaccaggc
    10261 gatgacctcc tgaagcacct tgtagatttc atccgcgagc tggcggattt tcgagaagat
    10321 ggtttcgagg gccgcgccgg cctgcttgat ggtcgagatg acgaacggct ccaaatcatt
    10381 aaagacgata tttaacacat cgtcggtgac cgtggcggca aaataggtca gctgatcgaa
    10441 ctcgtgctta aagaagtgcg ctacatcccc gaagatgccg ccggatgagc ctaactggcc
    10501 ggtggcccgc cggttgcgca acagatcacg cgcttcctct tccgtgagca cgcggaaccg
    10561 cgggccctcc ggatgggcga agtcgatctc ccaatgcggc gttttgatct ggctcagatc
    10621 gatagccccc tgccccgtgg agtccgtcgg gttttggttc atccaattcc ccgcggaatt
    10681 gatggcctgc gcggcggtgt tagccgggcc ggtttcctga taatcccctc ccgtcaaacc
    10741 ttgattgatc aggtccgtgc cggtcaccgt ttgcagttgc ggag
//
LOCUS       DAORAQ010000085        10697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_997_length_10697_cov_64.574610, whole genome shotgun sequence.
ACCESSION   DAORAQ010000085 DAORAQ010000000
VERSION     DAORAQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_10697_cov_64.574610"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..4098
                     /locus_tag="PKM72_08755"
     CDS             49..4098
                     /locus_tag="PKM72_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNP60915.1"
                     /translation="MHRLSSKIVIGLLLGIGICAALAYVLFPFIKTDVYRETLERGFS
                     ATLGRKVSLEGPISLTFSLQPRLILENVLVANPPWASQPHFFRANRLEIELSLVLLLQ
                     RRLEAEKIGLEGAELFLEEGSEGLDNWTFGDGAAPGIWSRAVPSVFMTISNRGEISIE
                     GSRIRYQPYLGEETTEVFIQQGVVVAPDDQLRAFSFEGAYRDVPVKIELNGGPIMDLF
                     NLDQAPWPVEGVLSVKEASASFKGMVGGTDINKTFDLSVQVHGSQLSALNDLLETDLP
                     DSAPFIIAADYLQGNDGITLNNVRGTLGTSDLAGQLIIPNGEGRQAIQGKLSSKIFQI
                     QDFLFSPKSTQQDMEAGQGASPTKPSPDTPASLPVDVDLDVTIEKCLFGEIEVGSVSL
                     TASGREGHVDLSPVRVKAFEGTAAARVKVELNTPQPQSVFEAKGTSLNYGQVFQALGV
                     TENIAGSTDFEVTASGSVATLEEFLKTVTMNLQLAHTTFGLSTPIPENPLPVALRQAS
                     LQISNGGPVKIVAQGGYQERAFGVRLMTAPPIGLIKTGTSLPVSLKALSGGALLEVKG
                     TLDIGKQDIPGTMSVSLKGSRLSEFDPDLPPVGPYVLMAHVSKKGDRFQVSDLRSRFG
                     SSDLSGRLELDLQKARPHLTGILTSKQINATEFSRPGEKSESAIPSEVMRGFDVDVNV
                     AIDHVRGEGLELAGLAFMAGLQEGRLKVDSLQGTVRDQRSTYGSFHGRFQMDTTGPIP
                     TLSGNGSFDGIRYDHFFPNVQFADLTKNDMTLDVNFSSLGNTIHSLLNRSTVLVEGEN
                     LSVRFHPKGDHPEPVQVSSNLKVESVDGGPLRLYAEGVFEKTPFRLRSATGPMSDILK
                     FGGLWPVNVRMDVPKAMVEMSGHVHFSHPGDEFDFQVLLKGQHLSDLSALTMSDLPVV
                     GPVDINLHLSRTPVGFHLTNIEGALGENSWGGNLTVLTKEERTRVRGKLIAESIDLGT
                     LQEPQGDASQHKNKSRVETIAGSVKGIGSAAFDTVRDTIGLKKKPKVSNIKAIPEFTF
                     PVEALRSFDLILDGEIKQIRKGEEDLGHVSFQVTLEEGVFTMQPVTGNLWGGDFDGKL
                     ILDGKQYVPTLEVNLNIHGLDYGHLAKSFGGTDMVKGQSQSIMLDLKGRGDTMYEVLE
                     QASGQFNLIDGPLELATRYIDLWAADLITTALTTAWKSESVSKLNCTVGYFDIEEGVL
                     KSDNILIDTSRLTIAGVGRLNLADETMDLLLTPRPKDPSLFSLAHMVRITGPLSDPDV
                     SSDKLRIAESGGWGLLGLVNPMGWIIAIPQIAGTTVGTMNQNPCVEAMKSRQHTAQAL
                     VEIKGGLWGTIKRAFSSLGGSSKVPPDNP"
     gene            complement(4675..6330)
                     /locus_tag="PKM72_08760"
     CDS             complement(4675..6330)
                     /locus_tag="PKM72_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNP60916.1"
                     /translation="MIVHVSPPPALAQALSALQDNSQETLHEKSIDDTVVPPILVPTA
                     PVILDGETLFHVRGVSALPAEERARRILERIDEFGSDLSRSTQSLRLVEEKGVTMIFA
                     GDQPLTGVTNADGRLEGGVEREVLARIYLKRIAEAVETHREKRKPINIVIHIAFSLLA
                     TAILVSAWWGIRWIFDKLDAVMEPRYKEQVKDLKFQKVIVLQAHQLWAGLRGGMHGIK
                     AFLLLLLLYAYLNFVFRQFPWTAYIGKQLFNIILDPIRVIVKGFLSSFPSLVFLVILI
                     VLLRYLLKFGKLIFANLFIGKVTWPGFDREWAWPTYRIFRTLVIAFGIVVGYPYIPGS
                     TSEAFKGITIFLGVLLSLGSSSIIGNILAGYTLVYRRAFQEGDRIKVNEILGDVMERR
                     ILTTRLRSLKNEEIIVPNSEILNNHVINYSAAAREKGLILHTTVGIGYETPWRQVEAM
                     LIEAADRTSGLLKDPRPFVLHLALGDFCVSYELNVHCDRPYYMMDLYTGLSQNILDVF
                     NEHGVQVMTPAYMADPEQPKIVPKDQWFTPPAKLPDKPKTGDT"
     gene            complement(7243..9528)
                     /locus_tag="PKM72_08765"
     CDS             complement(7243..9528)
                     /locus_tag="PKM72_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNP60917.1"
                     /translation="MKLMGMRKIIQLSLTVIIAWTLSGVSQAQEVLYDYPDLNPYAAT
                     VTETPIALRAVLPKKIPVKDYELRVFLDHKIPHVLWYDEKMRYSLVTQEGPAPLIFVI
                     AGTGAGYNSEKMTVLQHALYQAGFHVLSLSSPTHPNFIVSASSTGVPGHLLEDSQDLY
                     RVMRLAWLEHQSDIEVTDFYLTGYSLGAAQAAFVSKLDDEQGDFHFKKVLMINPPVSL
                     YTSTSILDDMLAKNIPGVADTFPEFFDKVFHAFSEVYRKGDFVDFSGDFLYTAYKDRE
                     PADEVLAALIGTSFRLSSANMIFTSDVLTNSGYIKPKNLVLSTTDSLTDYYKVSARVT
                     FLDYFREMFYPFFKIKYPDITEQSLIENLSLASLEDYLKHSTKIGLLHNADDFILGEG
                     DLDFLLSVFGSRAKIYPRGGHCGNLAHHTTLAYIVEYFNNPVSPNILKNNPEPFPAFT
                     SSESLRVEEQPLTQWAPVHNVTHSTNKENYPDLRHHLNQQSLSSGRGIQESQDSLEGT
                     TAMAAASEPSRTDPTLPVPRDNTPVVEQVIIEPARRPIQDVVQPGERYVIDVYDPIEG
                     FNRSMYKFNAKFDEYVFLPVVEGYEAVMPDFFEDRLSNFFSNIADLRNLLHSILQLKG
                     EVTLNTLSRILINSTFGLGGFFDHATPLGIHQQTEDFGQTLGHYGLNPGPYLVLPIFG
                     PSSIRDTTGLLADAAARYFYLYTPVNFDDHIDRSSAATLVWAVDVRHNLRFRYYQTGS
                     PFEYDLVRFLYTKKRQLEIEK"
     gene            9896..10129
                     /locus_tag="PKM72_08770"
     CDS             9896..10129
                     /locus_tag="PKM72_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60918.1"
                     /translation="MSDSYRFGDYQRVLGHGYEVFNFNALSPSVERDSEGHPISCQPP
                     RIPLTAEETFKLLRPYVSVRFMEQVKSVIPLGL"
     gene            10187..>10697
                     /locus_tag="PKM72_08775"
     CDS             10187..>10697
                     /locus_tag="PKM72_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HNP60919.1"
                     /translation="MSILFAVIVGLMFFMEGLKLRLMPFGTIIGQRLPEKSPLPLVLG
                     ITLLLGIGVTFVELVIGALKVAGQNVVVERAPYLYALLNHWSEALVLAIGFSVGLAAV
                     IGTMRFLYGWSLKPFIYGALAPVLALTLYGASDPDLTKVLGLAWDAGAVTTGPVTVPL
                     VLALGIGIAG"
BASE COUNT         2632 a         2515 c         2876 g         2674 t
ORIGIN      
        1 tggcttttgt ggattcaagc atgtattaat tgaagtctag gaattcttat gcatcgtctt
       61 tcatccaaaa ttgtgatcgg gcttcttctc ggcataggaa tctgtgccgc cctggcgtat
      121 gtgttattcc cctttatcaa aacggacgta tatcgggaaa cacttgaacg ggggttctcg
      181 gcgaccctgg gaagaaaggt ctccttggaa ggaccgatct ccctgacttt ttccttgcaa
      241 cctcggttaa ttctggaaaa cgttttggtt gccaatcctc cctgggcatc tcaaccgcat
      301 tttttccgag ccaaccgtct tgagatcgag ttgtccttgg tgctgcttct tcaacgccgt
      361 ttggaggcgg aaaagattgg tcttgaggga gcggaactgt ttttagaaga agggtctgaa
      421 ggtctcgaca actggacatt tggtgacggc gctgcgcccg gcatatggtc acgagccgtg
      481 ccgtcggtct tcatgactat ttccaataga ggggagatct ccatcgaagg ttcccgcatt
      541 cggtatcagc cttaccttgg tgaagaaacg accgaagtgt tcattcaaca aggggtggtg
      601 gtagcccccg atgatcagct cagagcattc tcctttgaag gcgcctatcg ggatgttccc
      661 gttaagattg aacttaacgg cggcccgatt atggacttat ttaacctgga tcaagctccc
      721 tggcctgtcg agggcgttct gtctgtaaag gaggcatccg cctccttcaa aggaatggta
      781 ggtggaacgg acatcaataa gacatttgac ctttcggtcc aggttcatgg gagccaactg
      841 agtgccctca acgatctttt ggagaccgac cttcccgatt cggctccctt tattattgcg
      901 gccgactatc ttcaagggaa cgatggcata accttgaaca atgtccgtgg gacgctgggt
      961 acttccgatc tcgcgggtca attgattata ccaaatgggg aggggcgtca ggcaatccag
     1021 ggtaaattgt cctcaaaaat tttccagatc caggattttc ttttttctcc aaaaagcact
     1081 cagcaagaca tggaggccgg gcagggagca tctcccacga agccatcacc agacaccccg
     1141 gcttcgctgc ccgttgatgt ggatctggat gtgacaatcg aaaaatgctt attcggagag
     1201 atagaggtgg gttccgtctc tttgaccgcg tctggtcgtg agggtcatgt tgaccttagt
     1261 ccggttcgag tcaaggcttt cgagggaact gcggcggcgc gcgtaaaagt tgaactcaac
     1321 acccctcagc cgcaatcggt gtttgaagcc aaaggcacct ctttgaacta tgggcaggtg
     1381 tttcaggcac taggagtgac cgagaacatt gccggatcta ccgactttga ggtgacggcg
     1441 agcgggagcg ttgccaccct ggaggagttt cttaagacgg tgaccatgaa ccttcaattg
     1501 gctcacacca cgttcgggct ctccactccc atacccgaga atccgctgcc cgttgccttg
     1561 cgtcaggctt ctcttcagat ttctaacggg ggaccggtca aaattgtcgc ccaaggtggc
     1621 tatcaagaac gggcctttgg tgtcagactc atgaccgctc ctccaattgg tctgatcaag
     1681 actggaactt ccttgccggt gtcactcaag gcattgtccg gtggggcgct tctggaagtc
     1741 aaaggcactc tggatatcgg caagcaggat ataccaggga ccatgagtgt ttcgctcaaa
     1801 ggaagccggt tgagtgagtt tgatcctgat cttcctcccg tgggtcccta tgttctcatg
     1861 gctcatgtga gtaagaaggg tgaccggttt caggtcagtg atctccgaag tcgatttgga
     1921 agcagcgatc tgtccggaag actggagctg gatctccaga aggccaggcc tcatctcact
     1981 gggatcttga catcgaaaca gattaatgca acggagtttt cacgacctgg cgaaaaaagt
     2041 gaaagtgcca ttccatctga agtgatgcgg gggttcgatg ttgacgtgaa tgtggcgatt
     2101 gatcatgttc gaggggaggg actggaatta gccggtctgg cttttatggc cgggcttcaa
     2161 gagggtcggc tgaaagtgga ctccctgcag ggaacagtac gggatcagcg atcaacctat
     2221 ggaagttttc atggcaggtt tcagatggat actaccgggc cgattcctac tctttccggc
     2281 aacggatcct ttgacggtat ccggtacgac catttcttcc cgaatgtgca gttcgcggac
     2341 cttacaaaaa atgatatgac cctggatgtc aacttttcaa gtttaggaaa caccatacac
     2401 agtttgctga atcgatccac cgttctggta gaaggggaaa acctatcggt acggtttcat
     2461 ccgaaaggtg atcatcctga accggtccag gtgagttcca atctgaaggt tgaaagcgtg
     2521 gatggcgggc ctttgcgact gtatgcggag ggggtatttg agaaaacccc ttttcggctt
     2581 cgttcggcaa ccggtccaat gagcgacatt ctgaaattcg ggggattgtg gccggtgaat
     2641 gttcgaatgg atgtgccaaa ggccatggtt gaaatgagcg gacacgttca tttctctcat
     2701 cctggcgatg aattcgactt tcaggtcctc cttaaaggac aacacctgag tgatctgagt
     2761 gcattgacga tgtccgacct gcctgtagtc ggtccggtgg acattaacct gcatctttcc
     2821 cggacgccgg tgggatttca tctgacgaac atcgaagggg ccctggggga aaactcctgg
     2881 ggaggcaatc tgacggtgct gacaaaggag gaacgtacac gggtgagggg gaaattgatt
     2941 gcagagagta ttgatctggg tactttgcaa gaaccccagg gggatgcatc acaacataaa
     3001 aacaaatcga gggtggaaac tattgcgggt tcggtgaaag gcatcgggtc tgcggccttc
     3061 gatacggtac gggataccat aggcctcaaa aaaaaaccga aagtttccaa cattaaggcg
     3121 attccggaat ttacgtttcc ggttgaagct ctccgttcct ttgatctcat acttgatggg
     3181 gaaatcaaac agattcgaaa gggagaagag gacctggggc atgtctcatt tcaggtcact
     3241 cttgaagagg gtgtcttcac gatgcaaccc gtgacgggaa atctatgggg aggagacttt
     3301 gatgggaaat tgatccttga tggcaaacaa tacgttccga cccttgaggt caatctgaat
     3361 attcatgggc tggattatgg gcacctagct aaatctttcg gtggtaccga catggttaag
     3421 gggcaatccc aatctatcat gctcgacttg aagggtcgag gagatacgat gtatgaagtg
     3481 ttggagcagg ctagtggaca atttaacctg atcgatggac cccttgagct ggccacaaga
     3541 tatattgatc tctgggctgc tgatttgatt acgacggcct taaccaccgc ctggaaaagt
     3601 gaatcggtat ccaaactcaa ttgtacggtg ggatattttg atatcgaaga gggagtattg
     3661 aaaagcgata atattctcat tgacaccagt cgactgacga tagccggcgt cggaagattg
     3721 aatctcgccg acgaaaccat ggatctactc ttaacaccac ggcccaaaga tccaagttta
     3781 ttcagtttgg ctcacatggt tcgcatcacc ggtccgcttt cggatccgga cgtttcaagc
     3841 gacaagttac gcattgccga gagcggaggg tgggggttgc tgggtctggt gaatcctatg
     3901 ggatggatca ttgccattcc acaaattgcg ggaacgaccg tgggcacgat gaatcaaaat
     3961 ccttgtgttg aggccatgaa aagtcgacag cataccgctc aggcccttgt tgaaatcaag
     4021 ggcggcctgt ggggaactat caaacgggct tttagtagcc ttgggggatc ctccaaggtt
     4081 cccccagaca acccatagta gagaaatcta tttgtgaaca gacttgtgtg atagcattcc
     4141 ttttgacttc ctcctttgcc tcccgttgtc ccgggaggat gtggagagaa ggagatccca
     4201 acccgattcc ataataattt cttctttctg ccagacgaat ctagtgccta tgtaagagct
     4261 atagcttgca cgatcagtca tattaaattt ctttcaatcc tggcaggaat ctcgccatgc
     4321 tgatttcata taaccagttg ctaagaagac ggtgctgggc gtttttcagt ggtagacgat
     4381 ctgcaagttc aagggggcat gagcgaacga cgggtagttt tttaatcaag acgttatcgc
     4441 tcgatccggt attttactcc atgccctcgg cccgtgtttg gttcaggccg acaacaacag
     4501 aaggccaggg gaatgagagc gagttccgcc aagttctgat ttgaatatcc ccacaaccgg
     4561 tatgtgtggg cggtggcttc acactcagca gtcaactaaa ggggtgactg aattcctgga
     4621 gaaaattgcg cgtggaaaaa acttccattc acttccccat ccacaggatt gaggtcaagt
     4681 gtcaccggtt ttaggtttgt ccggaagttt ggccggcgga gtaaaccact gatccttggg
     4741 tacgattttg ggttgttccg gatccgccat ataggccggt gtcatgacct gcacaccatg
     4801 ttcgttgaac acatccagaa tattctggct aagaccggta tagaggtcca tcatgtagta
     4861 tggccggtcg cagtggacgt tgagttcata gctgacgcag aaatcgccca aggcaaggtg
     4921 caggacgaaa ggccggggat ccttcaataa accggatgtc cggtccgccg cctcaatcag
     4981 cattgcttcg acctgcctcc acggggtctc gtagccgatt cccaccgtgg tatgaagaat
     5041 gagacccttc tctctggcgg cggcactata attgattacg tggttgttca gaatttccga
     5101 gttgggcacg atgatctcct cgtttttcaa cgaccgaagg cgagtcgtca gaattcttct
     5161 ttccatcaca tcgccgagaa tttcattgac tttgattcga tctccttcct gaaacgcccg
     5221 ccggtaaacc agggtatacc cggccagaat attgccgata atggaggagg acccgaggga
     5281 caagagtacc ccgaggaaaa tggtaattcc tttaaaggct tcggaggtgg atccgggtat
     5341 gtaggggtat cccaccacaa tcccaaaggc gatcaccagt gtccggaaaa tccggtaggt
     5401 gggccaggcc cattcccggt cgaaccccgg ccatgtcacc ttcccgatga acagattggc
     5461 aaaaattaat ttcccaaact taaggaggta gcggagcaaa acgatcagaa tgacgagaaa
     5521 aaccaggctg ggaaacgagg acagaaatcc cttcacgatt actcgaatag ggtcgaggat
     5581 gatattgaac aactgcttgc ctatataggc ggtccaaggg aattgacgga acacaaaatt
     5641 gaggtaagca tacagcagca aaaggagcag gaacgccttg attccgtgca tgcccccccg
     5701 tagtccggcc cacaattgat gggcctgaag aacaatgacc ttttgaaatt ttaaatcctt
     5761 gacctgttct ttgtatcgcg gctccatgac ggcatccagc ttgtcgaaaa tccatcggat
     5821 tccccaccag gcagaaacaa gaatagccgt ggcgagtaag gaaaaagcaa tgtgaatcac
     5881 aatgttgatg ggtttgcgtt tctcccgatg cgtttccacg gcttccgcga tccgttttag
     5941 atagattcga gctaagactt cgcgttccac tccaccttcc agccggccgt cggcattggt
     6001 cactccggtt aatggttgat cgcccgcgaa aatcatggtg accccttttt cctcaaccag
     6061 ccgcagggat tgagttgacc ggcttagatc ggagccaaac tcatcaatgc gctcaaggat
     6121 tcttcgggca cgttcctcgg cgggcaaagc cgacacgccg cggacatgaa agagagtttc
     6181 cccgtcaagt atgacgggtg cggtggggac cagaatgggt ggaacgaccg tgtcatcaat
     6241 ggacttttcg tgcagagttt cctgggaatt gtcttgaagg gcagaaagtg cttgggccaa
     6301 agccggcgga ggactgacgt gtacgatcat gatccagaat gcaataaggc ctggaacaat
     6361 aagagcaatt cgcctcaact tccgataggg taagaaaacc gatgaagagt tttgggaaag
     6421 ggaggggggc attgttcgtc tttcttcaat caaagaatct cagccccaaa aaggctcgcg
     6481 caataagaga gaagggcttg ttccggtgag tctctcatgg aagaatgtct tccttgaccg
     6541 acagaatgct cccttcaagg aaaccacctc aatccggttt gaagaacggt cggctccgtg
     6601 gagattaatt ttcaaattcg aatgtgcgtc ctgtttgaaa taaaggagcg gataggatca
     6661 tactcgaaag gggaatccgt ttggtagtag caaaaggcca accagggcgg tgtatcggca
     6721 gatcccaaca agcgattcac cctcttcccc ttgtgtattt ggaggacgta gctccttagc
     6781 tttcccttcc cgtgaaaaac cctatccttc ggggggattg atcctcacga aatgtatatg
     6841 atcatggtga aactcgatga cctgaaatcc tgcaaggggg gcgcagctgg gtggaggttg
     6901 agaaccggcc attcaggatc taaccatcgt catcccatgt ggttctgttc ctcaaatatc
     6961 ggagatggtg gtcatggcac ggcgtcgtcc atttcctgaa ttttgtactc cacaggaacc
     7021 gggagctggc atcatcggac cagatggcga gcaggggaat ttctccggcg tcagatggca
     7081 gcttggtgga ttgaagaagg ttcaccggtc attccatctc ctatgcagag acgaccgaat
     7141 gaccggaaat aataattcat ctggaccaga gaacatattt cacttttaat gtcgcgatga
     7201 tcatcgcatg ggggatacgc cgacaggaag cttcatggct atctactttt cgatttctaa
     7261 ttggcgtttt ttggtataga gaaaccggac cagatcatac tcgaatggag agccagtctg
     7321 gtagtagcga aagcgcaggt tgtggcgtac atccacggcc cacaccagcg tggccgcaga
     7381 gcttcgatca atgtggtcgt caaaatttac gggtgtatag aggtaaaagt agcgggccgc
     7441 cgcatcggct agcaagccgg tggtatcacg aatggaagac ggtccgaaaa tggggaggac
     7501 caggtagggg ccgggattca ggccgtaatg tccgagcgtt tgcccgaaat cttctgtctg
     7561 ttggtggatc cctagcggag tggcatgatc aaagaatccc ccgaggccga aggtactatt
     7621 gatcaaaatc cgtgacagag tattgagcgt gacttctcct ttgagttgaa gaatggaatg
     7681 caacagatta cgaaggtcgg cgatgttcga gaaaaaattc gaaagacgat cttcgaaaaa
     7741 atccggcatg acggcttcat atccctctac aacggggagg aacacgtact catcgaattt
     7801 cgcattaaat ttgtacatcg accggttaaa tccttctatt ggatcataga catcaatgac
     7861 atagcgttcg ccgggttgca ccacatcctg gatcgggcgc cgggctggtt caatgatcac
     7921 ctgttccaca acaggagtgt tgtcgcgagg tacgggaaga gtggggtctg tccgggatgg
     7981 ttcagacgcc gctgccattg ccgttgtccc ttcaagagag tcctgggatt cttgtattcc
     8041 tcggcccgag ctcagagact gttggttgag gtggtggcga agatcgggat agttttcctt
     8101 gttggtgctg tgcgtgacat tgtggactgg agcccattgc gtcagcggtt gctcctccac
     8161 gcggagactt tcagaggagg tgaatgccgg aaacggctcc ggattgttct tgaggatatt
     8221 gggagatacg ggattgttaa agtattcaac gatgtaggcc agggtggtgt gatgcgcaag
     8281 atttccgcaa tggcctcctc gtgggtagat tttagcccgg gagccaaaca ccgacaatag
     8341 gaaatccaga tcaccctcac ccagaatgaa atcatccgcg ttatgtaata atccgatctt
     8401 ggtggaatgc ttcagataat cttccaggct ggcaagactc aggttttcaa tgaggctttg
     8461 ttccgtaata tcggggtatt taattttaaa aaacgggtag aacatttcac gaaaataatc
     8521 cagaaatgtc acgcgagcag acactttata gtagtcggtc aaggaatcgg ttgtagagag
     8581 aaccagattt ttcggcttga tataaccgga attggtcaac acgtccgacg taaagatcat
     8641 attggctgaa gaaagccgga atgaggttcc gatgagcgct gccagaactt catcggcagg
     8701 ttcccgatcc ttataggcgg tatacagaaa gtctcctgag aaatcgacga agtcaccctt
     8761 ccgatacact tccgaaaatg catgaaacac cttgtcaaaa aactccggaa acgtatcagc
     8821 gactccggga atatttttcg ccaacatgtc atccagaatg gaagtcgaag tatacaagct
     8881 gaccggcggg ttaatcatga ggactttttt gaaatgaaaa tctccctgtt catcatccag
     8941 ctttgacaca aaggctgcct gagccgcgcc cagactgtaa ccggtgagat aaaaatccgt
     9001 gacttcaata tcgctttgat gttccagcca ggccaaacgc atgacacgat acaagtcctg
     9061 tgaatcctcc aggagatgtc cgggaactcc cgtgctggaa gccgagacga tgaaattcgg
     9121 atgggtaggg gaagacagag acagaacatg gaaaccggct tgatataagg cgtgttggag
     9181 aaccgtcatt ttttcggaat tatagcctgc ccccgtcccg gcgatgacaa aaattaacgg
     9241 ggcgggacct tcctgggtga ccagggaata gcgcattttt tcgtcatacc agagaacgtg
     9301 aggaatctta tgatcaagga ataccctcag ctcataatcc ttcacgggga ttttctttgg
     9361 aagcacagcc ctcaaggcga tcggcgtttc cgtaacggtt gctgcgtagg gattcaggtc
     9421 cggataatca tacaggactt cctgtgcctg actgactccc gacaaggtcc aggctattat
     9481 gaccgtcaaa gaaagctgaa taatctttcg catacccatc aatttcattt tcacctatcc
     9541 tctataatta tttatcgaaa accctgaaat gccgaaaagc cgctaacttt gccagatgat
     9601 tcaagccatt tcaatagagg ggaatcatgt cgggcagggg gccggtaaaa aattttccca
     9661 actcaatctt cgtcgaacgt acagcgatcc tgttatcgct tggagtattt cattgttatc
     9721 ttttgccttg gggtcttaat cccggcatcg gtattgttga aaacccagtt tcagagtaag
     9781 cgctgcatct atcgtattca aaaaaggaaa agccattttt gcttttcttc tttcccggaa
     9841 agattttgat aaggtatact cctgcaaccc actctagtcc atcaaacagc caaccatgtc
     9901 ggattcctat cgattcggag actaccaacg cgtactgggg catggatacg aggtattcaa
     9961 ctttaatgcc ttgagtccaa gtgtggaacg ggattcagag gggcatccca tatcctgcca
    10021 accgcctcgg atacccctca cggctgagga aaccttcaag ctccttcgcc cctatgtttc
    10081 cgttcgattc atggaacagg tcaaatcggt tattcccctg gggctgtagt tagcattgtt
    10141 ccaagttttg atattgcgcc aactggtgca gaacagctgg gtgatcgtga gtatcctgtt
    10201 tgcagtgatc gttggattaa tgtttttcat ggagggcctc aagctccgcc tcatgccctt
    10261 tggcaccatt atcggtcagc gtctgccaga aaaatcacct ttacccttgg tattaggcat
    10321 tacccttctt ctcggaatcg gtgtcacttt tgtcgagctt gtcattggtg cattgaaagt
    10381 ggccggccag aatgtcgtgg ttgagcgtgc cccctatctg tatgctctcc tgaatcactg
    10441 gtctgaggcg ctggtgctgg cgattggatt cagtgtgggc ttagcggcgg ttattggcac
    10501 catgcggttc ttgtatggtt ggagcctcaa gccgtttatt tatggggctc tggctcctgt
    10561 actcgccttg acgctctatg gagcctctga cccggatttg acgaaggtgc tggggttggc
    10621 ctgggatgcg ggtgcggtga caacgggacc ggtcaccgtg ccgttggtcc tggcattggg
    10681 gatcggcatt gcggggg
//
LOCUS       DAORAQ010000086        10565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1013_length_10565_cov_67.419886, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000086 DAORAQ010000000
VERSION     DAORAQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1013_length_10565_cov_67.419886"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKM72_08780"
     CDS             <1..207
                     /locus_tag="PKM72_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60920.1"
                     /translation="VIDILAFSDGVLRQVGFNWNADNDLEMLRQFWDCTAQAQLSLII
                     SHNGPGFIWRFRKTVHDSSRKVAL"
     gene            complement(314..802)
                     /locus_tag="PKM72_08785"
     CDS             complement(314..802)
                     /locus_tag="PKM72_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60921.1"
                     /translation="MAFSQVTPEEVIHFKCPPNDEQVFPVQGTLANFSPSSLLVTLKD
                     SKPNPAVSPKMQPSFRNVQTTSLTFSHQEAVIRVEEVQAFMTVPSTYHRIAINYQNLE
                     PSNPNNNENQVTQPGPKTSPNNKAEPPMTMDSLNSQLRKLQKIVDQQAEEIRRLQDSS
                     SR"
     gene            complement(1239..1985)
                     /locus_tag="PKM72_08790"
     CDS             complement(1239..1985)
                     /locus_tag="PKM72_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNP60922.1"
                     /translation="MRKLSPEQFRDRIFDVLRRKHHWATPFLEGSTITKEKLNIYFHQ
                     EYVVYVRDFSVLLAQILGKNPPWEARRLLATIIYEEETGGFSLGQPHQELFLHMMNGL
                     GFDRAGFRDVELLASSRGYREWLNQICQEEDWSVGAAVLSIFIKGTSNDPEEVLYPKP
                     AQNQEEIEDIVRKHYLSQYQSLSPAFMDYIRAQHMFSAGSRKTVYDMVTHHTDESNDQ
                     VKVLSLLEEALNLWSRYQEGIARACGIRQI"
     gene            2125..2409
                     /locus_tag="PKM72_08795"
     CDS             2125..2409
                     /locus_tag="PKM72_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60923.1"
                     /translation="MQPINLADPGAILELLANVTLRGEGVTAENLMEYVMDEGFTEPT
                     YLSAKGEDSLAFYQGQPNAWGIYQIREWKRVLVISGGTGKIRRAQITETP"
     gene            complement(2507..2947)
                     /locus_tag="PKM72_08800"
     CDS             complement(2507..2947)
                     /locus_tag="PKM72_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60924.1"
                     /translation="MITHENIKISPPSLSEDGSISPQETEEFSKQTSSESQVTPWICR
                     RPVRITIYGFLVAFALWMLGWGVVPRLLDPTFEQHLQDKSVMAGMSREQVMDAWGSPY
                     QMNVSYTEKGIRREEWVYEDWLDSSTIKHRYLYFEEGKLIGGWY"
     gene            3010..3363
                     /locus_tag="PKM72_08805"
     CDS             3010..3363
                     /locus_tag="PKM72_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60925.1"
                     /translation="MFLEVEQNPNFEGSYFLRFKELGPPRSVSHVKSYDRISQGEWCA
                     VTGWCEDVDQPIRPAYAQRVEDSGSGSTFIVFGGNWGIRLKLESDTNNWNIEDPNQWG
                     EGYLSIGEERDLRYE"
     gene            complement(3408..4034)
                     /locus_tag="PKM72_08810"
     CDS             complement(3408..4034)
                     /locus_tag="PKM72_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60926.1"
                     /translation="MAVRSRKAALRVFSYYTFPDWEKYFKSLVSPGNGEDVYTYVRNG
                     IQVRYSFAYIPDLNEGKTIPTLYVQRCEVEFSPSVPLKSIPSLVPEFIPPTEQSAPTF
                     RSNLWVLIFKGEPSREADFIVKEQGKRNLAWSLAYQLFAINGIPSVLSIDTPIDRLEI
                     TTQSLQIVRTQQRLTHESTLNPYSPEFQESLSPPSPPIKSVPQPHYDD"
     gene            4356..8027
                     /gene="metH"
                     /locus_tag="PKM72_08815"
     CDS             4356..8027
                     /gene="metH"
                     /locus_tag="PKM72_08815"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNP60927.1"
                     /translation="MNTLEAALESRILVLDGAMGTMIQAHNLSESDFRGTRYANHVCD
                     VKGNNDLLTLTQPKIIEDIHVQYLQAGADIIETNTFNSNSISLADYQMEGLAYELNLA
                     AGQVAKRAVARVQAEQPGRTCWVAGAFGPTNRTASMSPDVNNPAFRAVTFDDLEAAYY
                     EQVRGLVEGGVDLLIVETIFDTLNAKAAFYAIARYCDEIQRQLPIMASVTITDLSGRT
                     LSGQLIEAFWNSISHANLLSVGINCALGAKQMRPYIEELSNVAPIYISCYPNAGLPNA
                     FGGFDETPERMGADLRDFASQGWVNIVGGCCGSTPEHIRAIAEGVKDCAPHKKTHVPR
                     MTRLSGFEPLTIRPESNFVNIGERTNVTGSPKFSKLILNGKLEEALAIARQQVEGGAQ
                     IIDVNMDEGLLDSEKAMVEFLNLIGSEPDIARVPIMIDSSKWSVIEAGLKCIQGKGVV
                     NSISLKEGEAQFLEQAHLIRRYGAAVVVMAFDETGQADTLDRKVEICTRVYRLLTEKL
                     NFPPEDIIFDPNILTVATGMEEHNAYAVNFIEATRQIKATLPFCKVSGGVSNISFSFR
                     GNNTVREAIHSAFLYHAIKAGMDMGIVNAGQLGVYEEIPKDLLNLVEDVLLNRRSEAT
                     EELVTFAETVKQQGKTIVKDDAWRQGTVEERLAHALIKGIVEFIDADVEEARQKLNKP
                     LDVIEGPLMDGMNVIGELFGAGKMFLPQVVKSARVMKKAVAYLLPFMDAEKVEGESRS
                     QGKVLLATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVPCDKILQTARQEKVDLIGL
                     SGLITPSLDEMVHNAREMTREGFSIPLLIGGATTSKAHTAVKIAPGYTSPVVHVLDAS
                     LAVNVVRKLMSPDEKDAFVQEVQEKQRKTREAYLSKNTAKKFVSLEEARKQRFAIDWK
                     TTSIAKPTFLGVKVLEDVSLETLVPLIDWSPFFRAWQLRGKYPMIFEDSVVGPKAKEL
                     FDDAQKLLQEIIDRKLLTAKGVYGLFPANSQDDDITIYSNETRSEAVSVFHTLRQQVE
                     KPKGDPYYALADFVAPKSSGMADYIGGFAVTAGIGIEAVCQRFEKDHDDYNAIMTKAL
                     ADRLAEAFAEWLHREVRQVWGFGQDEQLTNDELIQEKYRGIRPAPGYPACPDHTEKQH
                     LFDWLQVEKTVGIQLTDSYAMYPTASVSGFYFAHPQAKFFSVGKLKKDQIEDYGKRKG
                     MDVSDVERWLSSNLGYDPT"
     gene            complement(8643..9155)
                     /locus_tag="PKM72_08820"
     CDS             complement(8643..9155)
                     /locus_tag="PKM72_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60928.1"
                     /translation="MSNTSMPGCRNWMSYLMDSLVNAGTMPSWEALQYLVTNLLGEAP
                     NPHLHQSKSPYETIQQFLHRIYGPIVEAASRTVEIPGQAMIHMFTDISLMGKSAVLVV
                     YFPGQNAPHQLLLLDSVPAWDLVFENSSAFDNWAEERYQWIVTALQSIKHPIESSMLE
                     CPDTLQPILV"
     gene            9512..>10565
                     /locus_tag="PKM72_08825"
     CDS             9512..>10565
                     /locus_tag="PKM72_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNP60929.1"
                     /translation="MRINYDKGGLIRPDKIQLPRSTNFKESSSKAKILLVFPPDWYPS
                     EPYLSLPTLTAFLRSAGHDVVQKDVNLEMYDWYFSRDFLRRILKKVPQQLDRLKKIGK
                     SRELTDAEVDLQLALCNCTRGYMSDLAERAEKAKEIVRSQEFYESEKLEWVMNTFREV
                     TATISLVYAPARICMPPMETDLSYKLFMSSEVLEAVEDTQVNVYRDVFEEILKPAILA
                     EKPDVIGISIVLRQQLFSSMTFCALIKEQFPDIHITIGGNTVTRLREVLPDTPKLFAL
                     FDSAIVYEGETALLRLLEAIGSDGDLSHVPNLLFRDAAGIHVNSESYAENMGELPPPD
                     FDGLPLEKYFVPEPILP"
BASE COUNT         2838 a         2088 c         2661 g         2978 t
ORIGIN      
        1 gtcattgaca tcttagcttt ctcagatggc gtgttacgtc aagtggggtt taattggaat
       61 gccgacaatg acctggaaat gcttcgccag ttttgggatt gcacggctca ggctcaactg
      121 tctcttatca tttctcacaa tggaccgggc tttatttggc gcttcagaaa aacggtccat
      181 gattcatcaa gaaaggtcgc cctttgatgc caatctacca aaatttcctc cgagctcggg
      241 tgagaggtca tgcccaggcc ggattgaatt cattcatggc agtaaatgaa tggatgtagg
      301 aaaatgattt attttatcga gaggaactgt cctgaagacg tcgaatttct tcagcttgtt
      361 ggtctactat tttttggagt tttcgtaatt gggaattcag tgaatccatt gtcatgggtg
      421 gttcagcctt attattaggg cttgtctttg gaccaggttg ggttacttga ttctcattgt
      481 tgtttggatt tgagggttcg agattttgat aattgatggc aatcctatga tacgttgatg
      541 gaactgtcat gaaagcttgg acttcctcaa cccttatgac agcttcctgg tgggaaaagg
      601 tcagagaggt tgtctggaca tttcgaaagg aaggttgcat ttttggagat acggcagggt
      661 tgggtttcga atcttttaac gtcaccagta gagaggatgg agaaaagttg gcgagcgttc
      721 cctggaccgg aaagacctgc tcatcgttcg gtgggcactt gaaatggata acttcctcag
      781 gggtgacttg agaaaatgcc atggatatat gatgtggcaa gaaatctatt tgtgagggag
      841 agaatgctgg aatggggtca taagttgaaa gcaggatttg ttggagtatg ccttcctctt
      901 gactaatggc tattcctttt agaattttgg cgataagaga ctctgaaaga attgctggat
      961 gactgggggt cttctttaga atgtgggatg tttttagtga aataatgcac tcgggcccgt
     1021 cctggagaac tgtttctact tcaggggtcc aagagcactg cagattgaag gttgttaggg
     1081 tagcgataag cagacatcgt ccggtaaatc ctctcggctt gaaatgatgt aatgaaggag
     1141 tgaatgcctg tagaaatgct tggatcactt caccagtggt tcagaagtaa ggatctagaa
     1201 ttttaaggac agcaatggag aaggaaagtt gatggtgctt aaatctgtcg aattccacag
     1261 gcacgcgcaa ttccctcctg atatcgtgac cacaagttca atgcttcttc gaggagagag
     1321 agaactttga cctgatcatt gctttcatct gtgtgatggg taaccatgtc atagacagtt
     1381 ttgcgggatc ctgcggaaaa catatgttgt gcacgaatat aatccataaa tgccggcgat
     1441 aagctttggt actgacttaa ataatgtttt cggacgatat cttcaatctc ctcctggttt
     1501 tgtgccggct tcggataaag cacttcttcc ggatcgttgg aagtcccttt aatgaaaatt
     1561 gagagcactg ccgctcccac cgaccaatct tcctcttggc atatttgatt caaccattct
     1621 cgatatccac ggctcgatgc caacaattcc acatcccgaa acccagctcg atcaaaccca
     1681 agtccattca tcatgtggag aaacagttcc tggtgaggct ggccaagtga aaacccaccg
     1741 gtttcttctt cataaattat tgtggcgagc agccggcgag cttcccaagg aggatttttc
     1801 ccgagaattt gagcgagaag cacagagaaa tctcgaacat agacgacata ttcctgatga
     1861 aagtaaatat tcaatttttc tttggtgatc gtgcttcctt ccaaaaatgg agtagcccaa
     1921 tggtgttttc ggcgaagcac atcaaatatg cgatcacgaa attgttctgg tgaaagtttt
     1981 ctcatattga cgggttatcc cttattacgg tctattttca tgaaaagaaa aaggtaggtt
     2041 ttgaataggc atgggattat ggtagacatg gtaagtgcct atgtaatgaa attatattat
     2101 cacatattaa aggagtcagt aacaatgcaa cccattaatc ttgcggatcc tggtgccatt
     2161 ttagaattat tagcaaacgt gactttgcgt ggcgaagggg tcactgcaga gaatctaatg
     2221 gaatatgtga tggatgaagg atttaccgag ccaacttact taagtgccaa aggggaagat
     2281 tctctggcat tttaccaggg gcaaccaaat gcttggggaa tttatcaaat ccgtgaatgg
     2341 aaacgagtct tagttatttc tggaggaaca ggaaagataa ggcgagctca aattaccgaa
     2401 actccttaag caaattccaa tcatgggtta gataagaatg gggagtgccc tggccttttc
     2461 tgatgagtcg tgcttgacca cagaatctgt ttgccaagca catttctcaa taccaacctc
     2521 ctattaattt cccttcttca aaatagagat atctgtgctt aattgtgctt gaatccagcc
     2581 agtcttcata gacccattct tctcgtctga tccctttttc cgtatagctg acattcattt
     2641 gataggggga gccccaggca tccattactt gctcacgact catgccagcc ataacagatt
     2701 tatcttgtag gtgctgttcg aaggttgggt cgagtaatcg aggtacgaca ccccatccga
     2761 gcatccatag cgcaaaggct accaaaaacc cataaattgt gatccgaact gggcgtcggc
     2821 agatccatgg cgtaacttga gattcgctgc ttgtctgctt ggagaactct tcagtctcct
     2881 ggggggaaat gcttccatct tcagatagag acggaggaga aatttttata ttttcgtgtg
     2941 ttatcataat taaggtaatt tactcattac tctagcaatg gatttcaagg tgggtcaagg
     3001 aaaaggtgta tgtttctcga agttgaacag aaccccaatt ttgaaggctc atatttccta
     3061 cggtttaagg agttggggcc tcctcggagt gtatcacacg tgaaatccta tgatcggatt
     3121 tcccagggag aatggtgtgc ggtgacgggg tggtgtgagg atgttgatca accaatccga
     3181 cctgcttatg cccaaagggt agaagattcc ggttccggtt caacatttat tgtttttgga
     3241 ggcaactggg ggattcgatt gaaattagaa agcgatacca ataattggaa tattgaagac
     3301 cccaatcaat ggggcgaggg atatttatca ataggagaag aacgggatct tcgatatgag
     3361 taacgctgag gagtggtcaa gttccataaa aaaatctttg cagaaggtca atcatcatag
     3421 tgaggttgag gaacggattt gatcggtggc gaaggaggag aaagactttc ctgaaattca
     3481 ggtgaatagg gattgagggt ggattcatgg gttaatcgtt gctgggtcct cacgatttga
     3541 aggctttgag tggtgatttc gaggcggtcg atcggcgtgt caattgaaag aacgctggga
     3601 attccattaa tagcaaataa ttgataggct agagaccatg cgagatttct ttttccctgt
     3661 tctttgacga tgaaatcggc ctctcgtgaa ggctcaccct taaaaatcag gacccagaga
     3721 ttggatcgaa aggttggggc cgattgttct gttgggggga taaattcagg gacgagcgaa
     3781 ggaatgcttt ttaggggaac cgaaggggaa aattccactt cacatcgttg cacatacagg
     3841 gttggaatgg ttttgccttc atttaaatca ggaatataag cgaaggaata ccgaacttgg
     3901 ataccattcc gaacgtaggt gtacacatcc tcgccatttc ctggtgaaac caaacttttg
     3961 aaatattttt cccaatcggg gaacgtataa tagctgaaaa cacgaagggc ggcctttcgt
     4021 gatcgaactg ccatcggtat tcctaatgtt tggcgtagtt cttcctgggt taaacctaaa
     4081 taaccatctt cgaagtaggg tttggctata gcgggggtag gccataagaa tccccctgtg
     4141 ctacaaagta gggaggccaa gatagcaaag gctacccatg aagtaggcct tttacaaagt
     4201 gagaagaaaa atgtcataaa aggatgaatg gcagatttgt catcctattt cttgaaattc
     4261 ctggaagtca agattaatga tgcttgaaaa tgagggaata gggctcagtt gttgaccgtg
     4321 agttgagatg tattttgatt gatgaggggg taagaatgaa cacactggaa gcggcgttag
     4381 aatctcggat ccttgtgctg gatggtgcca tgggaaccat gattcaagcc cataatttga
     4441 gtgaatctga ttttcgcggg acacgttatg ccaaccatgt ctgtgatgtc aaagggaata
     4501 atgacctgct gactctgaca caaccaaaga ttattgaaga tatccacgtg caatatctgc
     4561 aagccggagc cgatatcatt gaaacaaata ccttcaattc caattcgata tctctggctg
     4621 attatcagat ggagggtttg gcgtatgaat tgaatttggc ggcggggcag gtggcaaagc
     4681 gggctgttgc tcgggtgcaa gctgaacaac ctgggcggac gtgttgggtc gccggagctt
     4741 ttggtcctac aaatcgaacg gcctcgatgt caccggatgt gaataaccca gcatttcgag
     4801 cagttacctt cgatgattta gaagcagctt actatgagca ggtgcggggt ttggtggaag
     4861 ggggtgttga ccttcttatt gttgaaacca tttttgatac tctaaatgcc aaggccgcct
     4921 tttatgcaat tgcccggtat tgtgatgaaa ttcagcgtca attgcccatt atggcatcgg
     4981 taaccattac ggaccttagt ggccgtacgt tatcaggaca acttatcgag gccttttgga
     5041 attctatttc ccatgccaat cttttgagtg tggggattaa ttgcgcatta ggggccaagc
     5101 aaatgcggcc ctatattgag gaactttcta atgtggcgcc catctatatt agttgctatc
     5161 ccaatgcggg tttacccaat gcctttggcg ggttcgatga gacaccagaa cgcatgggcg
     5221 cggatctcag ggatttcgcc agccagggat gggtcaatat tgtcggcggg tgttgcggga
     5281 gcacacccga gcatattcgg gccatagccg aaggggtcaa ggattgcgcc ccgcacaaaa
     5341 aaacgcatgt tccccgtatg actcggttaa gtgggtttga gcctttaacc atcagaccgg
     5401 aatccaattt tgtcaatatt ggagaacgta cgaatgtgac aggttcgcca aaattttcaa
     5461 aattgatttt aaacggaaaa cttgaagaag ctttagccat agcgcgtcaa caagtcgaag
     5521 ggggtgcgca aattatcgat gtgaatatgg atgaaggcct tctggattcg gaaaaagcca
     5581 tggtggaatt tttgaatctc atcggatccg aacccgatat cgctcgtgtg ccaatcatga
     5641 ttgatagctc taaatggtcc gtcattgaag ctggcctgaa gtgtattcag ggaaaaggtg
     5701 tggtgaattc cattagtctc aaggaaggcg aagctcaatt ccttgagcag gctcacctga
     5761 ttcgtcgcta tggggccgct gttgtcgtca tggcatttga tgaaaccggt caagccgata
     5821 cgcttgatcg aaaagtggaa atctgtacta gggtctatcg tctcttgacc gaaaagctca
     5881 actttccgcc ggaggacatt atttttgatc cgaatatttt gacggttgca acaggcatgg
     5941 aagagcataa cgcctatgct gtgaatttca ttgaagccac tagacaaatc aaagccacat
     6001 tacctttctg taaagtcagt gggggggtca gtaatatttc gttttcattc cgtggaaata
     6061 atacagtgcg cgaagccatt cattctgcgt tcctctacca tgccatcaaa gcgggaatgg
     6121 acatggggat tgtgaatgcc ggtcagttag gagtctacga agaaataccc aaagatctcc
     6181 ttaatttagt agaggatgtc ctgttgaata gacggtcaga agccactgag gaattagtga
     6241 cctttgctga gacagtcaag caacagggca aaacgattgt gaaagatgat gcgtggcggc
     6301 agggaacagt agaggaacgt ctggcccatg ccttgattaa agggattgtt gaatttattg
     6361 atgctgatgt ggaggaagcc cgtcagaagc tcaataagcc tctggatgtc attgagggcc
     6421 cattaatgga tgggatgaat gtgatcggag agctatttgg tgcggggaaa atgtttttgc
     6481 ctcaagtagt caaaagcgcg cgggtcatga aaaaagctgt ggcgtattta ttgccgttta
     6541 tggatgcgga aaaagttgaa ggcgagagca gaagccaagg taaagtcctg ctcgcaacag
     6601 taaagggcga tgtgcacgac attgggaaga acatcgttgg agttgtactg gcctgcaata
     6661 attatgaaat tattgatctg ggtgtaatgg ttccttgtga caaaattttg cagacagctc
     6721 gtcaggaaaa agttgatctg attgggctca gcggcctgat tacaccttcc ttggatgaaa
     6781 tggttcataa tgcacgagaa atgactcgag aaggtttctc tatccctttg ttaattgggg
     6841 gagcgaccac gagtaaggcc catactgccg tgaaaattgc gccaggctat accagcccgg
     6901 ttgtacatgt cttagatgcg tccctggcgg tcaatgtcgt gcgcaaatta atgagtccgg
     6961 acgagaagga tgcatttgtg caggaagttc aagaaaaaca acggaaaacg cgagaggcgt
     7021 atctgtccaa aaacacggcc aaaaagttcg tatcactgga ggaagcaaga aagcagcggt
     7081 tcgccatcga ttggaaaacg acaagtattg ctaagccaac tttccttggt gtgaaagttc
     7141 ttgaggatgt ttccctcgag acccttgtcc ccctaattga ctggtctcca ttttttcgcg
     7201 cctggcaatt acgtgggaaa tatccaatga tttttgaaga ctcggttgtc gggcccaaag
     7261 ccaaagaact ctttgatgat gctcaaaagc ttttgcagga aatcattgac cgaaagctgc
     7321 ttacggcaaa aggtgtctat ggattatttc cagccaatag tcaggacgat gacatcacca
     7381 tctattccaa tgagacccgt tcggaagcgg tatcggtctt tcataccttg cgacaacaag
     7441 tagaaaaacc caaaggtgac ccgtactatg cgttggccga ttttgtggct ccaaagtcca
     7501 gcggtatggc tgattatatt ggtgggtttg cggttacggc cggaatcggc atcgaagctg
     7561 tttgtcagcg ttttgaaaaa gaccatgatg attataatgc catcatgact aaagccttgg
     7621 ctgatcgttt ggccgaagcc tttgccgaat ggctccatcg tgaggtccgg caggtatggg
     7681 gatttggcca agatgagcaa ttgacaaacg acgaactcat tcaggaaaaa tatcgtggaa
     7741 ttcgtccagc acccgggtac ccggcctgtc ccgaccatac ggagaaacag catttgtttg
     7801 actggcttca ggtagagaaa acggtaggta ttcaattgac cgattcgtac gcgatgtatc
     7861 ccactgcgtc tgttagtggt ttctactttg ctcaccccca agccaaattc ttttctgttg
     7921 gtaaattaaa gaaagatcaa atcgaggatt atggtaagcg gaaagggatg gatgtatcgg
     7981 atgtagaacg gtggctctcc tcaaatttag ggtatgaccc tacttgagta ttttggtgtg
     8041 cagcttttga cttttgaatt tacgggatcg ttctattaaa acctcaagga aagagccgga
     8101 agaggagtgc gatgatgctg gtttgaaagt tataagtgct aatctggatc agaatatcgt
     8161 tggccccgtt cacccgacca tttggtaatt ccgacctctt gagattccca taaatcctgc
     8221 atttcttggg ctgccaactc tgcttccttt gcccgctttt ccacttctgg tagtttctgg
     8281 aattcttttg aacgggcttg gtctggttcc gggccccttc ctcgcggtga atttttaagt
     8341 aatacattga ttggcatatt tacctcatat tgtttcgtaa ggtttcatgg atagcgcctc
     8401 gtcacttgga ctgtcaagtc tgatggaagg aaagactgtc tggctagagt agccggcaat
     8461 gaaagtccca tctaagtgag actcttgctt gtatttttgg tgctttcctt ccaagcaacc
     8521 ccacgaaaaa ttttgtcctg ggatggtcgc gttttaattg aggcttcttt aatggcccaa
     8581 gatgggagta gttatgccct acctggctct tccgcctgtg ccctaacaag tatgcttagt
     8641 ctttagacca gtatcggttg gagggtgtct gggcattcaa gcattgagga ttcaattggg
     8701 tgtttaatac tctgaagggc cgtcacgatc cattgatatc gttcttcagc ccagttatcg
     8761 aacgcagagg aattttcaaa taccaagtcc caggctggca ccgagtctaa tagtaacaat
     8821 tggtgcggag cgttttgtcc tggaaaatac acgaccaaaa ctgccgattt tcccatgaga
     8881 ctgatatccg taaacatatg aatcatggct tggcctggaa tttcaactgt gcgggaggcg
     8941 gcttcaacga ttggtccata aatacggtgg aggaattgtt gtatggtttc atagggggat
     9001 ttgctttgat ggagatgcgg attgggcgcc tcacctaaca ggttagtcac gagatattgg
     9061 agggcttccc atgagggcat tgttccagca ttgactagtg aatccattaa gtacgacatc
     9121 caattccggc atccaggcat gctggtattg ctcattgtca tttcctcacg gctcatttct
     9181 agggatccta agaaatttaa gagttgttga tgaagggtac gctaccagac aaggaaggaa
     9241 ttccaagggc agataagtga ttttaatatg aagatattat tcggtcaaat aattattcgg
     9301 tcaaatattt taaattttcc tattacggtt ctcctatgca attgcccttg accatggttt
     9361 tttcaaggac ttagaatggt gaatctatct gatgtaaaaa atatttaaaa tcaatgggtt
     9421 agtttagatg tgaatataga tgaataggtt tatcatcatt ctggtttgat tgacctaatc
     9481 gcgagtaaac cattttacct agggtgtaaa aatgaggata aattatgata aaggtggttt
     9541 gattcgccct gataaaattc aattaccaag atcaaccaac tttaaagagt catccagcaa
     9601 ggccaaaatc ctgttggtct tccccccgga ctggtatccc tctgaaccct atctgagcct
     9661 tccaacccta acggcttttc ttcgatctgc cggtcatgat gtcgttcaaa aagatgttaa
     9721 tttagaaatg tacgactggt attttagtcg ggatttcctc cgtcgcattc ttaaaaaagt
     9781 tcctcagcaa cttgatcgcc tcaaaaagat tgggaagagt cgtgagttaa cggatgcgga
     9841 agtggatctt cagttagctc tctgcaattg tacccgaggc tatatgagcg atttagctga
     9901 aagagctgag aaagcaaagg aaattgttcg aagccaagag ttttatgaaa gtgagaagct
     9961 ggaatgggtg atgaatacct ttagggaggt gactgccaca atatccttgg tgtatgcccc
    10021 ggcaagaatc tgcatgcctc ccatggaaac tgatttatcc tataagctgt ttatgtcgtc
    10081 cgaagtgctg gaagccgttg aagatactca agtcaatgtc tatcgggacg tatttgagga
    10141 aattctgaag cctgccattc tagctgaaaa gcccgatgtc attgggattt caattgttct
    10201 tcgacaacaa ttgttttcct ctatgacatt ttgtgcactc attaaggagc aattccctga
    10261 tattcatata accattggag gcaataccgt aacccgcctg agggaagtgc ttccagatac
    10321 tccaaagtta ttcgccttat ttgacagcgc gattgtctat gaaggtgaaa ctgccctgtt
    10381 gcgattgttg gaagccatag gctcggatgg ggatttatcg catgttccta atttactatt
    10441 cagagatgca gcgggaattc atgtgaattc cgaatcctac gccgaaaata tgggcgagtt
    10501 gcctcccccg gatttcgacg gattgccatt agagaaatac tttgtacctg agccgattct
    10561 tccct
//
LOCUS       DAORAQ010000087        10406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1026_length_10406_cov_74.203845, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000087 DAORAQ010000000
VERSION     DAORAQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_10406_cov_74.203845"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKM72_08830"
     CDS             <1..211
                     /locus_tag="PKM72_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60930.1"
                     /translation="NDAHGVVQIGQTHFIFNGNLRQTWFNRHTSLLERDHRTSCSAHS
                     RDLVRPIAPCTQDHAKDSTFGSTER"
     gene            complement(229..2733)
                     /gene="gyrB"
                     /locus_tag="PKM72_08835"
     CDS             complement(229..2733)
                     /gene="gyrB"
                     /locus_tag="PKM72_08835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNP60931.1"
                     /translation="MESETNPSQESAHSADRQHSDVRYDADQIRVLEGLEAVQKRPAM
                     YIGSTGVDGLHHMVYEVVDNSVDEHMAGYGSEIQVVLQVDGSVLVSDNGRGIPTGLHS
                     TQKKSAAEVALTVLHAGGKFEEGAYKVSGGLHGVGISVVNALSEWLELEIWQNGEVFE
                     QRYHRGKPQTPLTVAGITKKRGTKVVFRPDPLIFETVEFSFDVLAQRLRELAFLNKGL
                     EICLKDDRAGKEKEQVFCYIGGIVSFVEHLNEAKSPLHPPIVVEVEKPEMILQLALQY
                     NEGYSENLYSFANNINTREGGTHLIGFKSALTRTINSYATANDLFKKDSESLSGEDVR
                     EGLTAVVSVKIRNPQFEGQTKAKLGNSEVKGIVESAVNEALGSYLEENPSVAKKIVGK
                     SVDAARAREAARKAKELIRRKGALDGGSLPGKLADCSEKDPQFSELYLVEGDSAGGSA
                     KQGRDRRSQAILPLKGKILNVEKARFDKMLASEEIRTLIMAIGTGIGRPREDAEKGKE
                     DKDAFDINRARYHKIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYIYIAQPPLFK
                     VKKGKGEKYLKDEAAMNTYLSNLAVEDTQLFLPEQNEFVTRDELIPILDKLVAFEGLL
                     TRQGQKQIEPALLRVLVDFPQLTKDLLKNRGDLEVLIEEATRRLRLAFPEGTVDLTIQ
                     TDEEHQAHHILCRVAGNGSQKFLNLTHDMVGSADFRELQKISPSALGLGRPPYRLKRK
                     DTEAEFSTSQDLVTEFLETGKKGMSIQRYKGLGEMNPTQLWDTTMNPETRSLLQVTLE
                     DTTGVDEIFTILMGDEVEPRRNFIQRHALEVRNLDV"
     gene            complement(2790..3914)
                     /gene="dnaN"
                     /locus_tag="PKM72_08840"
     CDS             complement(2790..3914)
                     /gene="dnaN"
                     /locus_tag="PKM72_08840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNP60932.1"
                     /translation="MKIQITREDLLTALQRVQGVVEKRNTMPILANILLEAKPEGLDI
                     VATDLEIGMRGLYKATVHEPGSVTFSARTLFDILKEIRHSDIELVVGENNWVTVKAGK
                     SQFRVVGLPSKDYPALPTIEREGLMALPAQGLLQLLKKTVFAVGEKDTRYVLNGLLLT
                     LIPTGATVTLRLVGTDGHRLAWAEQEVTPEKAAIPEAEIKVIIPKKAAIEIRRLLEED
                     DEQPFLGFTKNMLIFRKSGLVLTSRIMEGTYPNYQQVVPKESSKKITVNKSDLEGALR
                     RVAILSKDKAHAVKLSINPDHIHLSSKSPDLGEADEDIPAKFVGEGFSTGFNARYFLD
                     VLSVIETESLTLQMETPLSPCLIQEQGNPAFKAVVMPVKV"
     gene            complement(3937..5346)
                     /gene="dnaA"
                     /locus_tag="PKM72_08845"
     CDS             complement(3937..5346)
                     /gene="dnaA"
                     /locus_tag="PKM72_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNP60933.1"
                     /translation="MSLLLTSVNKIVNKLNLPNINNEKVWVDVLNFVSPRVGDDTIET
                     WFQPLVLEGLNEDQAQIRVPNRFFGEWLGRNYKDLLKEAFQYVEGVNPAEIIFVMKEQ
                     DGEGKPLPELKTEVRETRGPVQLQRVRRQPLPNPKYTFKNFVVGASNQFAHAACLAVA
                     ESPAKAYNPLFIYGGVGLGKTHLLNSIGNYIADHGDLRIAYVTTEQFTNEVINSIRYD
                     KMIELRRRYRNVDMLLIDDIQFLAGKERTQEEFFHTFNSLYEAGKQIVLSSDRFPKEM
                     PSMEERLRSRFEWGLIADLQPPDVETRIAILRKKSEEEGIAINEDVIQLLAANLKSNI
                     REIEGALIRLGAYASLTGQKITVDMAKHILRDLLGGKRKVITTEDIQEVVANRFHVKI
                     SDLKSKRRTKTLVYPRQIAMFLSRDMTDSSFPEIGRDFGGKDHTTIIHACKQMQKAQE
                     TDSTLRATIESLKEEIGKG"
     gene            complement(5866..6210)
                     /locus_tag="PKM72_08850"
     CDS             complement(5866..6210)
                     /locus_tag="PKM72_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60934.1"
                     /translation="MPKNLVILIREDPTLTHRASEAMRIALGLSTGPNPVKIILLENA
                     RQLISDEAYDLPDGEILEKYLPSIKNLELPIVVPIGSGKTFSLDQEFTIQESTPSQIA
                     SLVSQADRVLTF"
     gene            complement(6382..7632)
                     /gene="hisS"
                     /locus_tag="PKM72_08855"
     CDS             complement(6382..7632)
                     /gene="hisS"
                     /locus_tag="PKM72_08855"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNP60935.1"
                     /translation="MLRSIKGLKDILPQQTPQWSYMEHLAHTLATQSGFSEIRIPIFE
                     STELYARSIGGATDIVEKEMYTFPDRDGSSLTLRPEGTAGVVRAVLEHHLLESPSTRK
                     LYYLGPMFRHERPQAGRYRQFNQFGVEAFGTEDPFMDVEVIALLWRFFSSLGLKDLTL
                     HINSIGNPQDRENYIQELKRFILPKLELICGNCQRRYSTNPLRILDCKVPICQEATQD
                     APTLFDHLSPESASHFNTVLDGLTSIDIPFSMNYRLVRGLDYYTRTTFEITTPHLGAQ
                     STIGAGGRYDGLVALLHGPSTPAIGFAVGLERVALLLSNHLSTPHPPLFFVAGFGKDA
                     KPMALKLLHTLREAGIRADTDYKSVNLKSLLRSADKLGASYAIILGDDEARSETAILR
                     NMTTKAQESVKLKEISQKILSLTS"
     gene            7820..7966
                     /gene="rpmH"
                     /locus_tag="PKM72_08860"
     CDS             7820..7966
                     /gene="rpmH"
                     /locus_tag="PKM72_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNP60936.1"
                     /translation="MSMTYRQPSNLKRKRDHGFRKRMSTKNGRKVLARRRKKGRQRLT
                     VSDE"
     gene            8337..8525
                     /gene="yidD"
                     /locus_tag="PKM72_08865"
     CDS             8337..8525
                     /gene="yidD"
                     /locus_tag="PKM72_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNP60937.1"
                     /translation="MIAGYQYGISPFMGICCRFEPTCSHYGLEAIHKHGAIKGLVLMA
                     SRILKCHPFHPGGLDPVP"
     gene            8600..10339
                     /gene="yidC"
                     /locus_tag="PKM72_08870"
     CDS             8600..10339
                     /gene="yidC"
                     /locus_tag="PKM72_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNP60938.1"
                     /translation="MEKRVVLFLVLSLGIIFGYDLLLKELGYSPFSTSPIIDQELKTS
                     LPAKGADAETISPSSSESHSVSSSNFSTEPLLTEEVVVVETPLFRAGISGQGGVLNSW
                     ELKKYLTQTEENVPVQLVYPNGQFPGPLTVQVKGNEVETQRLRQGNFQVQKDFNQLDE
                     AHPTGKVLLTYSSSDSELWVQKELTFHYDSYLVDITIRTRGISGDIDVLLGTNFGVVE
                     WGQGFIGLLGSAWMVGEEMEKVSPDADAPILRRDGPVRWLALQDKYFMSVFIPENAKS
                     LFSKLETEMVVSAGMSLSGGSGEQVHKTKLYAGPKKYDVLKSFNIGLEDTIDFGWFIY
                     GSWSIVKAVAKPLFSVLRFIYDYTHNYGVAIILLTLGIKLLFVPLQYKSYKSMQGMQT
                     IQPKVLALQEKLKDNKEKLNTELMKLYKEHKVNPVGGCLPMVLQMPVFVALFNILYMT
                     IDLRQAPFMLWVTDLSAQDPYYVLPVLMGISMVVQQKIMPTTMDPTQAKMMMILPVGL
                     TFLFVTFPAGLVLYWVTNNVLTVTQQFVTDRYILKKPRGGSTDPSPKGSEPALESGKK
                     KRSSSPSGPSSKS"
BASE COUNT         2673 a         2477 c         2324 g         2932 t
ORIGIN      
        1 caatgacgct catggcgtag tccaaatagg acaaacgcat ttcatcttca atggcaactt
       61 gcgtcagacg tggttcaacc ggcatacgtc actcctcgaa agagatcatc gcacttcctg
      121 ttctgcacat tcacgggact tggtccggcc catcgctccc tgcacgcaag accacgccaa
      181 agattcgacc ttcggttcaa cagagcggta ggcaaacgaa ggcacctgct agacatctaa
      241 atttcgaact tccagggcat gtcgttgaat gaaattccgg cggggttcaa cttcatcacc
      301 catcaaaatg gtaaagattt catccactcc ggtggtatct tctaatgtca cctgcagaag
      361 actgcgggtt tccggattca tggtggtgtc ccacagttgt gtcggattca tctcgcccaa
      421 acccttgtag cgctgaatgg acatgccctt tttcccggtt tccaaaaact ctgtcacgag
      481 atcctgactg gtggaaaatt ccgcttccgt gtctttgcgt tttagccggt aagggggtcg
      541 tcccaatccc aacgccgaag gagagatctt ttgtaattcc ctgaaatctg cagaacccac
      601 catgtcgtga gtcaggttca aaaacttctg actgccattc cctgccaccc gacagaggat
      661 gtggtgggcc tgatgctctt catcggtctg aatcgtcaga tccacggtgc cttcggggaa
      721 cgccagtcga agccgacgag tggcctcctc aattaatact tccaagtcac cacgattttt
      781 taacagatct ttcgttaatt gtggaaaatc caccaatacg cggagcagcg ccggttcaat
      841 ttgcttttgc ccctgacgag ttaagagccc ctcaaaggcc accagcttgt ctaaaatcgg
      901 gataagttca tctctggtca cgaattcgtt ttgttccgga agaaagagtt gcgtatcctc
      961 cacggcgaga ttggacagat aggtattcat tgccgcttcg tctttgagat acttttctcc
     1021 cttccctttt ttgaccttaa acaagggagg ctgagcaatg tagatatagc ctcgctcgat
     1081 gagttccggc atttgccgga aaaagaaagt cagaagcagg gtccggatat ggctgccatc
     1141 gacgtccgca tcggtcatga taatgatttt gtggtaccgg gctcgattga tatcaaaggc
     1201 gtctttatct tccttgcctt tttcagcatc ttcccgtggc cgccctattc cggtgccgat
     1261 cgccatgatg agggttcgga tttcttcact ggcaagcatt ttatcaaatc tagctttttc
     1321 gacattcagg attttgccct tgagtggaag gatggcttgt gaacgtcgat cacgcccctg
     1381 tttcgcagac cctcctgccg aatcaccctc tacgagatac agttcactga attgcggatc
     1441 tttttctgag cagtcggcca attttccagg aagtgacccc ccatccaacg cgccttttct
     1501 gcgaatcagc tctttggctt ttctggcggc ttccctggcc cgtgccgcat ccacagactt
     1561 cccgacgatt ttttttgcta ccgagggatt ttcctcaaga tacgatccca aggcttcatt
     1621 gacggccgac tccacaatgc ccttaacctc actattgcct aatttggctt tggtctggcc
     1681 ctcaaattgc ggattgcgga ttttcacact caccacggcg gtcagacctt ctctcacatc
     1741 ctctccgctc agcgattctg aatctttttt aaacaaatca ttggcggtcg cgtaactatt
     1801 gatggtgcgc gtcaaggcgg acttaaatcc gatgagatgc gtgccgccct cgcgggtatt
     1861 aatattattg gcaaaggaat acaaattctc cgaataccct tcgttatatt gaagggccaa
     1921 ctggagaatc atctcgggtt tttccacttc cacgacaatg ggaggatgaa ggggagattt
     1981 ggcttcattc agatgttcga cgaaggagac gattcctcct atgtaacaaa atacctgctc
     2041 cttttctttt cccgcccgat catcctttaa gcagatctcc aaccccttat tcaggaaggc
     2101 caattcacga agtcgttgcg ccaatacatc aaaactaaat tccacagttt caaaaatgag
     2161 gggatccggc ctaaagacca cttttgttcc gcgtttttta gtgatccccg caaccgttaa
     2221 tggggtctgc ggcttcccac gatgatatcg ttgttcgaat acttccccat tttgccaaat
     2281 ttccaattcc agccattccg aaagggcatt cacgacggat atcccgacac catgcaatcc
     2341 tcccgacacc ttataggcac cctcctcaaa ctttccgccg gcatggagaa ctgttaatgc
     2401 gacttcggca gccgattttt tctgtgtgga atgcaggccg gtgggaatcc ctcttccatt
     2461 atccgagacc aggacactac catccacctg aagcacaacc tgtatttcac ttccatagcc
     2521 tgccatgtgt tcatccacac tgttatcaac aacctcgtac accatatgat gcaggccatc
     2581 gacccctgtg ctgccgatat acatggcggg ccgtttttga accgcctcca gtccttccag
     2641 gacacggatt tgatcggcat cgtaccgaac atcagaatgt tggcggtctg ccgagtgggc
     2701 agattcttgg ctagggtttg tttcggattc catacaattt taggctttgg ctacgtgtgg
     2761 aagaagaaat gtctgttggg tcaaatccgt caaaccttca cgggcatcac caccgctttg
     2821 aacgcgggat ttccctgctc ctgaatcaaa cacgggctta agggtgtttc catttgaagg
     2881 gtcagcgatt ccgtttcaat gacagataac acatccaaaa aatatctggc gttgaatccg
     2941 gttgaaaagc cttctcccac aaatttagcc gggatgtcct catctgcttc tcctaaatcc
     3001 ggactttttg aagaaagatg aatgtgatcg gggttgatgg acagtttcac ggcatgggcc
     3061 ttatctttcg aaaggatagc tacccttcga agggcaccct ccagatcgga cttattgacg
     3121 gtaatttttt tggaactttc tttcggtacc acctgctggt agttgggata ggtcccttcc
     3181 ataatccggg aagttaacac caacccgctt ttccgaaaga ttaacatatt tttggtaaac
     3241 ccgaggaacg gttgttcgtc gtcttcttcc aggagtcgcc gaatttctat ggcagctttc
     3301 ttgggaataa tgaccttaat ttcggcttcg gggattgcgg ctttctcggg agtcacttcc
     3361 tgctcagccc aggctaaccg atggccatcg gtgcccacca gacgaagtgt caccgtggct
     3421 cctgtaggaa tcagggtgag aagaagcccg ttcaacacat aacgtgtatc tttttcgccc
     3481 acggcaaata ccgtcttttt taaaagctgc aacaaccctt gggcggggag cgccatcaac
     3541 ccttcccgtt caatagttgg aagggcaggg taatccttac tgggtaagcc caccacacga
     3601 aattggcttt tcccggcctt caccgtcacc caattatttt cacccacgac caattcaatg
     3661 tcggaatgtc tgatctcttt taaaatatca aaaagtgttc gagcggagaa tgtgaccgac
     3721 cccggctcat gaaccgtggc tttatataat cctctcatgc caatttctaa atcagtcgcc
     3781 acaatatcca ggccttccgg cttggcctcc aacagaatat tcgccaggat cggcatggta
     3841 ttccgcttct ccaccacacc ctgcacccgt tggagtgcgg ttaacaaatc ctccctggta
     3901 atttgaattt tcatatgtgt cctatgaacg gtggttctat cccttgccga tttcttcctt
     3961 caggctttcg atggtggctc gaagagtgga atctgtttcc tgagccttct gcatttgctt
     4021 gcaggcatga ataattgttg tatggtcttt tcccccgaaa tctcttccga tttccggaaa
     4081 ggaggagtcc gtcatatcac gactgagaaa catggcaatt tggcgagggt acactaacgt
     4141 tttcgtccgt cgtttcgact tcaagtcgga tattttgaca tggaagcggt tggcgaccac
     4201 ttcttgtata tcctctgtcg taatcacctt tcgtttccca cccaggagat cacggagaat
     4261 atgttttgcc atatccacag tgattttttg acccgtgagc gacgcatagg caccaagccg
     4321 aataagtgct ccttcaatct cgcgaatatt gctttttaaa ttcgctgcta acaactggat
     4381 gacatcctca ttgatcgcaa taccttcttc ttccgatttt tttcggagaa tggcaattcg
     4441 agtttccaca tcaggtggtt gaagatcggc aatcagcccc cactcaaacc gggaccgaag
     4501 ccgttcctcc attgagggca tttctttggg aaaacgatca ctcgacaaca cgatctgttt
     4561 tccagcctca tagagagaat taaatgtgtg aaaaaattcc tcttgagtac gttcttttcc
     4621 tgctaaaaat tgaatatcgt caatgagcag catgtccaca tttctatatc gccttctcaa
     4681 ttcaatcatt ttgtcatagc gtatggaatt aatgacctca ttagtgaatt gctctgttgt
     4741 gacataggca atacggagat caccatgatc ggcaatataa ttccctatag agttgagaag
     4801 atgggttttc cccaatccta cgccgccata aatgaataac gggttatatg ctttggcagg
     4861 ggattccgct acagcaagac atgccgcatg ggcaaattga ttacttgctc ccaccacgaa
     4921 gtttttaaag gtatattttg gattaggaag aggttgccgt cttacccgtt gaagttgaac
     4981 gggcccacgg gtttctcgaa cttcagtttt aagctcgggc aaaggtttcc cctccccatc
     5041 ctgctctttc atgacaaaaa taatctctgc cggattcacc ccttcgacat actgaaaagc
     5101 ttctttgagt aaatccttat agtttctgcc taaccattcc ccaaaaaatc tatttggaac
     5161 acggatctga gcctgatctt cattaagacc ctctaaaacc aaaggctgaa accatgtttc
     5221 aatggtgtca tcccccacac gtgggctgac aaaattcaac acgtctaccc aaactttttc
     5281 attgttaata ttaggtaaat tcaacttatt cacaatttta ttcacagatg ttaatagtaa
     5341 cgacaccaat catggtcctg ccatttttta ttagactctt tccgattcct atcaacaccc
     5401 tctaccataa acttcatgcc ctctatccac aacttccacc cagatatttt taaaactcaa
     5461 accactttcc ctacccaatt tcaaaggagt tttaaatttc aagatcaatt atccccccta
     5521 taccctatcc ctactaccac tactcctact gtttcttttt cttttttata acaaagaaga
     5581 agagggatag ttatatcacc aaggccaaat ccgacagaaa tattttttcc aaaaacccct
     5641 gataggaaat tgtctcagaa ggtcgggaat catctcctct ttctccttca cggcctaaaa
     5701 caaaaacctg ggaggttgga ataggcggta aactctgtcc acccttgagg agcaagaccg
     5761 ttacatcttt ctcagataga gaaggaaccg gttccgggat aaaaacattc cagtcatact
     5821 ggtctttcgt acagatatat aaaatatttc tcataagcga taccgttaaa aggtaaggac
     5881 tcgatctgct tgagaaacga gtgaggcaat ttgagatggg gtggactctt gaatagtaaa
     5941 ctcctgatcg agtgagaagg tcttcccgct ccctattggt acaactatcg gcaattctaa
     6001 atttttgata gaagggaggt atttttctag aatctcccca tcaggaagat cataggcctc
     6061 atcagaaatt aactgcctgg cattttctaa gagaataatc tttaccggat ttggaccggt
     6121 cgataatccc aacgcaattc gcatagcttc ggatgcccgg tgcgtaaggg tgggatcttc
     6181 tctgataagg ataaccagat tttttggcat cccttattta taccttggat taatacgtaa
     6241 aatactggat ttgttaatga aatatgcgga ggatattttt ttattcaaaa aatatcgttt
     6301 cttattgaaa aataagaaaa aaaccctagc accaggtaaa aaaaatgtca agaaaaaagc
     6361 caagatatct tatccaatca tttaagaggt taatgataga attttttgag aaatttcttt
     6421 cagcttcacg gattcctgag ctttggtggt catatttcta aggatggccg tttccgaacg
     6481 ggcttcatcg tctcccagga taatggcata actggctcca agcttatccg cggatcgaag
     6541 aagggattta agattgacac ttttatagtc tgtgtctgcc cgtatacccg cctctctgag
     6601 ggtgtgaagc agtttaagcg ccatcggttt agcatccttt ccaaacccgg ccacgaaaaa
     6661 taacggcgga tgtggggtgg agaggtggtt tgaaaggaga agtgcgactc gttccagtcc
     6721 cacagcaaac cctatggcag gggtggaagg tccatgcagt aaggcgacta atccgtcata
     6781 acgtcctccg gccccaatgg tgctttgtgc gcctaagtgc ggagtggtaa tttcaaaagt
     6841 cgtacgggtg tagtaatcca atcctctcac taatctgtag ttcatggaaa aagggatatc
     6901 tatagaagtc aatccgtcga ggacggtgtt aaagtgagag gcagattccg gggaaagatg
     6961 gtcgaaaaga gttggagcgt cctgggtcgc ttcctgacaa ataggaactt tacaatcaag
     7021 tatccttaag gggtttgttg agtaccgacg ttggcagtta ccacagatca attccagttt
     7081 tggcaaaata aatcgtttga gttcctgaat atagttttca cggtcttgag gattgccgat
     7141 tgaatttata tgcaacgtga ggtctttaag tcccagggaa ctaaagaatc tccataaaag
     7201 ggcaatcact tccacatcca taaagggatc ttcggtccca aacgcctcaa ccccgaattg
     7261 gttaaattgc cggtatcgac cagcttgcgg gcgttcatga cgaaacatcg ggccaaggta
     7321 atatagtttt ctggtagaag gagattccag gagatggtgc tccaatacag ctcgcaccac
     7381 gcccgctgtt ccttcaggac gtaaagtgag agagcttcca tctcgatcgg ggaaggtgta
     7441 catttctttt tctacaatat ctgttgctcc tccaatactc cgggcataga gttcagtgga
     7501 ctcgaatatt ggaatacgta tttctgagaa gccagactgg gtggcaagtg tatgggcgag
     7561 gtgttccata tagctccact ggggggtttg ttgaggtaaa atatccttaa gacccttgat
     7621 ggagcgcaac acgggaaacc ttgttctgtt gaagaaaatg ttgagggaaa agagaatccg
     7681 actatatctt tccgtcagaa tccagtcaac gaatcaatgg ggtatatgag tttaggatgg
     7741 gaaaatatgt ttgactctta ctctttcccc caataaaatg gcgtgttcat aagaaaatgg
     7801 ctaaaaggag aacaacgccg tgtcaatgac ctatcgacag ccttctaatt taaaaagaaa
     7861 acgagatcat gggtttagaa aacgcatgag cactaagaat gggagaaagg ttcttgcccg
     7921 ccggcgaaag aagggccggc aacgtttaac ggtttctgat gagtaagagg tgggcttccc
     7981 gtttttttta aagaaaaaat atgaatacga tggagtgaag tccaatgggt ctgctgtgcg
     8041 gaacccattt tttaccctcc ttgttagtcg tgtacccaac catgatccgt cccgtgtagg
     8101 aattatcgta gggcgacgag tggggaaagc cgtgacgaga aatcggctga aacggatttt
     8161 ccgtgaactc gtaagagaat cgcactccgg catggcgagt ggttttcatt gcatcgttta
     8221 cccaaaaaca aagattttgg gagtgaaata tcagaaggtt ggagaaacct ggaagggcac
     8281 attggcccaa gtaggtcttc tttaatgaaa atatggtagc ttctgtttta aaaaaaatga
     8341 ttgcgggata ccaatatggg atatccccat ttatgggaat ctgttgccgt tttgaaccaa
     8401 cctgttctca ttatggctta gaggctatcc ataaacatgg cgcgataaag ggtttggtat
     8461 taatggccag tcgaattctc aagtgtcatc cattccatcc tggggggctg gatccggttc
     8521 cctgaatgct gaacattttg cctccgtttg tttgattgtt aattaccacc agctttaatg
     8581 agactcacga gttagaaaaa tggaaaagcg cgtcgtcctt ttccttgttc tttccttagg
     8641 gattatcttt ggatatgacc tgctcctgaa ggagttgggc tattctcctt tctctaccag
     8701 tccaatcatc gatcaagagc tgaaaacgag tttgccggcg aaaggggcgg atgcggagac
     8761 gatctccccg tcttcatctg aatcacattc agtatcttct tctaattttt ctactgagcc
     8821 acttcttacc gaagaggttg tggttgttga gacaccactt ttccgagctg ggatttcagg
     8881 gcagggagga gtgttgaact cctgggaatt aaaaaaatat ctcacccaga cagaggaaaa
     8941 tgttcccgtc caattggtat atcccaatgg gcagtttccc gggccactca ccgtgcaggt
     9001 caaaggcaat gaggtggaga cccagcggct tcgtcaaggg aactttcaag ttcaaaagga
     9061 ttttaaccaa ttagatgaag cccatcccac cggtaaagtg cttttaacct attcctcttc
     9121 cgattccgaa ttgtgggtcc aaaaagaact tacctttcat tacgattcct atctggtgga
     9181 tatcactatt cgtactcggg ggatttcagg ggatatcgat gtgcttttgg gcaccaattt
     9241 tggtgttgtt gaatggggtc aaggatttat tggattgctg ggatctgcct ggatggtcgg
     9301 ggaggaaatg gaaaaggtgt ctcccgatgc ggatgctccg atcctgcgtc gcgatggtcc
     9361 tgtgagatgg ttggcccttc aggataaata ctttatgagt gtctttatcc cggaaaatgc
     9421 gaaaagcctg ttttccaaac ttgaaactga aatggttgtc tcagcgggca tgtctctttc
     9481 agggggatca ggagaacaag tccataagac aaaattgtac gccggtccca aaaaatacga
     9541 tgtccttaaa tcttttaata tcgggttgga agataccatt gattttggat ggtttattta
     9601 tggcagttgg agcattgtga aagccgtggc gaagccgctg ttttcagttt tacgtttcat
     9661 ttatgactat acccataatt atggtgtggc cattatttta ctgaccttag ggattaaact
     9721 gttatttgtt cccttgcaat acaaaagcta caagtccatg caaggaatgc aaaccatcca
     9781 acccaaagtg ctggcccttc aggaaaaact caaagacaac aaagagaagc tcaacacgga
     9841 actcatgaag ctttacaaag agcataaagt gaatcctgtc ggtgggtgtc tccccatggt
     9901 gttgcagatg cccgtgtttg ttgcgctctt taatattttg tatatgacga ttgatctcag
     9961 gcaggccccg tttatgttgt gggttacaga cctttctgcc caagatccgt attatgtgct
    10021 gcctgtgctg atggggattt ctatggtcgt ccaacaaaaa atcatgccca ccacgatgga
    10081 ccccacccag gcaaaaatga tgatgatcct gcctgttggc ttgacatttt tgtttgtcac
    10141 ctttcccgca ggtttggtgt tatattgggt gaccaataat gtgttgaccg tcactcaaca
    10201 atttgtgact gacaggtata ttttgaaaaa accgagaggt gggtctacgg atccttctcc
    10261 aaaagggagc gaacccgctt tggagtctgg gaaaaagaaa cgctcatcat ctccctcggg
    10321 tccttcctct aaatcctagt ccttgcctct gtctaagcta tgggctcttt agacgacacc
    10381 atctgtgccg tggccacccc tatggg
//
LOCUS       DAORAQ010000088        10172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1050_length_10172_cov_67.371157, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000088 DAORAQ010000000
VERSION     DAORAQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_10172_cov_67.371157"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKM72_08875"
     CDS             <1..533
                     /locus_tag="PKM72_08875"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNP60939.1"
                     /translation="SGGTVNIWRWNAEWQKDLGAGVAGMWDVDNQYPSIAWDYYYEEP
                     AGGVTYPDRIGRSLGPFNPGIWSGNIMSDPNLRLGSVEDLNANGFSTLTTQASQDVVG
                     NGLWEPYGSLKGGCCSGPTWRVVMKRSLKTQDPNDVQFAAGASFPVAFAVWDGSNVER
                     NGMKGISTWFTAQMPN"
     gene            786..2558
                     /locus_tag="PKM72_08880"
     CDS             786..2558
                     /locus_tag="PKM72_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP60940.1"
                     /translation="MKISLLFPPSWHPSQPYLSVPCLSAFLEKSGVPVHQQRDLNIEL
                     LDRVLTKGFGLDIHPRLVDLVKKLEESVSGETGYGSKEHYARVVESLDRFPYLIDEIE
                     PVKASLRSEEFYDLERYRECLFLIDRWLEVVSSLYFPTRLTVVDNQFAYSIYSSREIL
                     QAIHDESQNPYLDLYRKFFLPAFGEDLPNFVGVSITATSQIIPGLTLCKLIKEIHPDV
                     HVTVGGSIFTRLVDNLRRCDRLFQLTDDFIVFEGETALLELINQLDGKRDFSKVPNLI
                     WRQDGKIIVNQPFYSENLAEHPAPNYDGFPLGEYLAPHTVLPVQFSRGCYYKDCAFCA
                     LTLDHQNFRQKDPMKVVDELEALSNKYETPYFFFTDECLALSPTKRLCKELIHRGLDL
                     QWTAELRFEKNLSRELLASMREAGCQKIVFGLETYNARLMDFMVKGITQGNVDRICSD
                     CVDLGIAVHCYVIVGFPTETEEESLETLNFIVNNTKLHSSYGFSCQPCLFDLEKEAPI
                     MSDPGGYGIQRIMRPASEDLSLGFFYEVKSGMTPEQAEKVYQYVYEKISEVVCELPFN
                     YSMADGLLYITRHNKQVEVEPSRV"
     gene            2628..3740
                     /locus_tag="PKM72_08885"
     CDS             2628..3740
                     /locus_tag="PKM72_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60941.1"
                     /translation="MISTTGKSQERIDGPGLLFEWGLKSLLFAAFFELVLYRLVSRLG
                     MHLGKLAEKYEAVRIGFRTLSSLGFVLLNMTAILVFLILGILLVQKIRTKLWVGEWDK
                     FVIPLAALLVACTVGYLLFPPVMVGTIFYNVLAFGLLVFLVLEYWATHRVLSQRLMVG
                     TFFLGISGWLYYQTMSTTYGWLNIFDAPPLVHEGNRLGEALMVLSSILVFWAYGTTSI
                     ITKNKQQRRWGLTLVISGGSAFLALLFMDFFLSLWNPEVAKEVRKAGEGIGWIFQMGM
                     GYTFYLPFAFYVLGFTCWAYTVIKLVLIGRLAGYGLGLMFMAGYALQLSHLTLMVVLG
                     LMLLNIDKKGTLGKLRKKTQEGVVYQPIRPVYGQTQ"
     gene            3755..4570
                     /locus_tag="PKM72_08890"
     CDS             3755..4570
                     /locus_tag="PKM72_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP60942.1"
                     /translation="MEEQSAASQTMEPQSGETTTEERPLTVDEEIAEIEKLLSEEPDD
                     FQARCRLGELYFNKGRMDEALAEVKKSIEMAEGIRVEMDRSLAMYYSNLGTIYGTKGM
                     LEEATAQFKRALELNPYDVLALFNVGRLYNDKNAFLEAKEYFERLIEISPEDPIAWYN
                     LAGVYEKLDDPNISDFNTLDMAMQAYMKVLQFDPSNLESSFKLMEIALNLKKSDLAVK
                     IMEDAVENNPDEPLAYYNLINTYEKCKMFEQAEETRNRLKDRFNKRSRETVKK"
     gene            4593..4916
                     /gene="tatA"
                     /locus_tag="PKM72_08895"
     CDS             4593..4916
                     /gene="tatA"
                     /locus_tag="PKM72_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNP60943.1"
                     /translation="MFGSLGFTELILILMIVLIIFGAGKLPQLGEGVGKAIKGFKKSV
                     HEAEAYDLDPQTQAENTASANHGNPQAQISQPMQTVQTETVPPIQTEAAHPAPQEQVP
                     ASSKA"
     gene            5008..6261
                     /locus_tag="PKM72_08900"
     CDS             5008..6261
                     /locus_tag="PKM72_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60944.1"
                     /translation="MSIETALEIGVIETFAGNGKSRSTGDGKRAVKSGIPLPNHVTVD
                     RDGRYFYIAESGSDRIRRVDMETGLLHNFAGTGETCYSGDYGPCGEAGLYLPLDVLCD
                     SQNHIYVCDSGSNRIRKIDADSGIITTIVGTGQWGFNGDGPALECNLTYPAAIAIDDQ
                     DHLYIADTQAHRIRHYNPQTGMITTIAGCWTAEDDERVSPLTAQNLIVLSGDAIGIDF
                     SNDEGLLRPRCSDGLDLSLYLDDGKSALECKLYDVVDIDVDRHGDLYITDKGTNRIRK
                     LDRKTGTMSTVAGICRYGFDGDGKLAVKSMLNVPEGAVVDSEGNVYISDSMNHRVRKV
                     DSQTGVITTIAGNGDSGYDDKNMGGCGAARFVAKEDAGMLKHGDGLIAVEAIVNTPAG
                     LTLDSQGYLYICERNENKIRRLKLR"
     gene            6622..7497
                     /locus_tag="PKM72_08905"
     CDS             6622..7497
                     /locus_tag="PKM72_08905"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNP60945.1"
                     /translation="MSTIHAKKRIVLFVVFAVVVLFGLTWYQVPLVSSQGMVVLSHFS
                     KGEVPTHPSDSAWESVAPLPVPLSGQIITRPVWPEPSARALSIRSIHNGKDIAFLLEW
                     QDATINESLTPGVFRDGVALALPLGDAPAFFCMGQLDHYVNIWHWKADWQSDVDRRAE
                     RAKESKREKSGPRRFEVIPRRPSSVEDLVGGGFSTLTSKEQQGRIQGQAEWKNGLWRV
                     VMKRPLTSTGDDLDNEAMLVPGRMQAIAFAVWNGENKERNGQKSVASWMQLLIDPVPL
                     SQNGPGVMESTHRSS"
     gene            7536..7967
                     /locus_tag="PKM72_08910"
     CDS             7536..7967
                     /locus_tag="PKM72_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNP60946.1"
                     /translation="MRIFLSILCLWFGLIALQAETFGQLPNENMTEEKAMILAEQFGI
                     DVGEVDEEIKKILGLTKAEGVVVYAVIGGSPAELSGIKVKAIIKEVDKYEIKTLVDLG
                     TALEQTLPTKNFTVATYEPADPENQGVTGGLNFHFVRILQD"
     gene            7977..9314
                     /locus_tag="PKM72_08915"
     CDS             7977..9314
                     /locus_tag="PKM72_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNP60947.1"
                     /translation="MENLFSKNFRLLKIVPLHNRWILTALLGIFCLSGVGLGQQPSSV
                     IGEGDESWIEEIEQVFIPSEQCKQCHDRHYEEWKGMREQTMDLKTFGRVDGALLHGTA
                     LTSPVFKTVLGLWLQTDPDQEQRTRCLSCHAPAVTVFPQHTDRIIDQVMKGGKRGKIE
                     GISCSACHLITGIQENAYGHPTFKIESGAILYGPYAEPDENLVHPSAQADIYRGAHYC
                     ASCHFDKVKDVTRPDIPGEILKGTICQDCHMERSTGSSTSQRGALTRPIGRHWFQGIV
                     IPGIMLSNRNLQAEWFSRVDIEAKKVNGQVEGEVLLRNGALPHTFPGGDPVLKQFYVT
                     ITLKNSDGQVIDQYQERFGRTFEELLRGPIPRPFVNGGTTRHIPFTLKVPQGTEVTLI
                     EAAVSYSLIPEPSKALSTKFIEGLATDKERETAERIIKDYSSPRLLTFRTMSL"
     gene            9407..>10172
                     /locus_tag="PKM72_08920"
     CDS             9407..>10172
                     /locus_tag="PKM72_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNP60948.1"
                     /translation="MRGGILLLLGCFLLAMPPQASVASESSLNKKPIEKSFPNSEKCK
                     RCHLRVFDEWEASAQSRSIVTAAFRVTLDKFLASSDKKDHAMCFRCHAPHILEYGDHL
                     PHFIKEVQSKDPRMDGVGCPQCHLIQDVDMNSHPPTPTFQLGTTIFGGYDKAAENLAH
                     QSQKLELYRESKFCVTCHDSLPKITDSAKDLPGWLGSWEKTNAETSGKPCQTCHMPEA
                     VDESANGEKVRKVANHSFPGRFGKVRADAVTLDFSTE"
BASE COUNT         2713 a         2012 c         2594 g         2853 t
ORIGIN      
        1 agtctggtgg gacggtcaac atatggcgtt ggaatgcgga atggcaaaaa gatcttggtg
       61 ctggcgtagc aggcatgtgg gatgttgaca atcaatatcc atccattgcc tgggattatt
      121 actatgaaga gccagctggt ggtgtgacct atcctgaccg aattggtcgg agccttgggc
      181 catttaaccc aggaatttgg tctggcaaca ttatgtcaga tcctaacctg cggttgggat
      241 cagtcgaaga tttgaatgca aatggattta gcacgttgac aacccaagcg tcacaagacg
      301 tggtgggaaa tggattatgg gagccgtatg gatcattgaa gggtggctgc tgcagcggcc
      361 caacctggcg ggtggttatg aagcgaagcc tcaaaaccca agaccccaat gatgttcaat
      421 tcgcggcagg tgccagcttc ccggtagctt ttgcagtctg ggacggttcc aacgtagaaa
      481 gaaacggaat gaaagggatt tccacttggt tcacggctca aatgccaaac taagttccag
      541 ccttttctgg atcaggtcca ttttcattta ggactttgat tgggtttgaa aacctccggt
      601 aatggactcc agtaatgggg aaaattaccg gaggtttttt tttaataaac atctactcct
      661 tatgaaatga aaatttgtct gatagggagt ggccaattgt tgcattgaag agaatgcgac
      721 gtgtataaat ttaaaataga caattttcgc gtttcttatt atccccatta cctgggagcc
      781 gcattgtgaa aatttcactt ttattccctc caagttggca tccctcacag ccctatttga
      841 gtgtgccctg cctttcagcc tttcttgaaa aatccggtgt tcctgttcat cagcaacgtg
      901 atttaaatat tgagttactt gatagggtct taactaaagg ttttgggttg gatattcatc
      961 cgcggttagt tgatttggtg aaaaagctgg aagagtccgt ttctggagaa accggctatg
     1021 ggagtaagga gcattacgct cgtgttgtgg aatcccttga tcgttttcct tatttaatag
     1081 atgaaatcga gccagttaaa gcctccttgc gtagtgaaga attttatgat cttgagcggt
     1141 accgagaatg cctctttttg attgatcggt ggttggaagt tgtctcttcc ctatatttcc
     1201 cgaccaggct gacggtagtt gacaaccaat ttgcgtattc tatctattct tcacgcgaaa
     1261 ttcttcaagc tatccatgat gaaagccaaa acccctatct ggatctttac cggaaattct
     1321 ttcttcctgc ctttggtgaa gaccttccaa actttgtcgg ggtctccatt accgcaacat
     1381 cacagattat ccctgggtta accctttgta aattaattaa ggaaattcat cctgatgttc
     1441 atgtaaccgt cgggggaagt attttcacca ggttggttga taatctcagg cgatgtgatc
     1501 ggttattcca attgactgat gatttcattg tttttgaagg agaaacagcc ctgttggaac
     1561 tgataaatca gttagatgga aaaagagatt tcagtaaagt gccaaatctc atttggcggc
     1621 aagatggaaa aatcattgtg aatcagccat tttactcgga aaacctcgcg gagcatccag
     1681 caccgaatta tgatgggttt cctttgggag aatatttagc gccccatacg gtgttgccag
     1741 tacaattttc tagagggtgc tattataagg attgcgcttt ttgtgcactg actttagatc
     1801 atcagaattt tcgtcaaaag gatccgatga aagttgttga tgaattggaa gccttatcca
     1861 ataaatatga aacaccgtat tttttcttta ctgatgagtg cctggcactt tcaccaacta
     1921 aacggttgtg taaggaactc attcatcgag ggttggattt acagtggacg gccgagttga
     1981 gatttgagaa gaatttatct cgtgagttac tggcctccat gcgtgaggca ggatgtcaga
     2041 aaattgtctt tggattggaa acctataatg ccaggctgat ggattttatg gtgaagggga
     2101 ttacccaagg aaatgttgat cgaatctgtt ccgattgtgt tgaccttgga attgcggtgc
     2161 attgctacgt gatcgtggga tttccgactg aaacggaaga agagtccttg gaaacattga
     2221 attttattgt caataataca aagctgcatt cttcctatgg attttcctgt caaccttgtc
     2281 tatttgatct agaaaaggaa gcccccatta tgagtgatcc agggggatat gggattcaac
     2341 gaataatgcg gccagcttcg gaagatctga gcttagggtt cttttatgaa gtaaaatcag
     2401 gcatgactcc ggagcaagct gaaaaagtgt accaatacgt gtatgaaaag atcagtgaag
     2461 ttgtgtgtga attaccattt aattactcca tggcggatgg gttgctatat atcacccgcc
     2521 ataacaaaca ggttgaggtg gaaccctcta gggtttagaa attatctggg atgaaaatct
     2581 agccatctca atacacacat gcgcttcaat ttctaatgta caaaaatatg atttctacta
     2641 ctggaaagag ccaagaaaga atagacggcc ccgggcttct ttttgaatgg ggtctcaaga
     2701 gtttgttgtt tgcagccttt tttgaattgg tgctgtatcg gctggtttca cggttgggta
     2761 tgcaccttgg caagttagcc gaaaaatatg aagcggttag aataggattt cgcacgctct
     2821 cttccttagg gtttgtacta ttgaatatga ctgcgattct ggtttttctg attttaggga
     2881 ttttgctggt tcaaaaaata cggacaaagc tttgggtggg agaatgggat aagttcgtta
     2941 ttccattggc ggccttattg gtagcctgta cggtgggcta tttactcttt cctccggtta
     3001 tggtcggaac gattttctat aatgttttgg cgtttggatt gctcgttttt cttgttttgg
     3061 aatactgggc tactcaccgg gtgttgagcc aacggctcat ggtgggtact tttttccttg
     3121 ggattagcgg atggttgtat tatcagacga tgtcaacgac ttatggatgg ctcaatattt
     3181 ttgatgcccc acccctggtg catgaaggta ataggcttgg ggaggcatta atggtgcttt
     3241 caagcattct ggtgttttgg gcctatggga ccacgtcgat catcaccaaa aacaagcaac
     3301 agcgccgatg ggggttaacc ctggttatta gcggggggag tgcctttcta gccctacttt
     3361 tcatggattt tttcctttct ctctggaacc ctgaagtggc gaaagaggtc agaaaggctg
     3421 gcgaggggat aggctggatt tttcaaatgg gaatgggcta taccttttat cttccctttg
     3481 cattttatgt gttgggcttt acctgttggg cgtataccgt gattaagttg gtgttgatcg
     3541 gtcggttagc aggatatggg ctgggactca tgtttatggc tggctatgcg ctccaattat
     3601 cccatttgac cttgatggta gtgcttgggt taatgttgct aaatattgat aaaaaaggaa
     3661 ctttagggaa acttcgtaag aaaacccaag aaggggtagt ctatcaacca attcgtcccg
     3721 tttacgggca aacacagtag ttaggacaca cgcaatggaa gaacaaagtg ctgcaagcca
     3781 aacgatggaa ccgcagtctg gcgagacgac cacagaagaa cgcccattaa cggttgatga
     3841 ggaaattgca gagattgaaa agttgttgag cgaggagccg gacgacttcc aagcacggtg
     3901 tcgattgggc gagctctatt tcaataaagg ccggatggat gaagctttgg cagaagtaaa
     3961 gaagtcgatt gagatggcag aaggcattcg ggtagaaatg gatcggtctc tggccatgta
     4021 ctattcgaac cttggaacga tttatggaac gaaggggatg ctggaggagg ctacggctca
     4081 atttaaacga gcattggaat taaaccccta tgatgtactt gctcttttta atgtcggccg
     4141 actctataat gataaaaatg catttttaga ggccaaggag tatttcgaac gtctgattga
     4201 aatttcacct gaggatccca ttgcctggta taatttggcc ggagtctatg aaaaattaga
     4261 tgaccctaat atctccgact ttaatacgtt agacatggca atgcaggctt atatgaaagt
     4321 actccagttt gatccaagta atttggaatc gagttttaag cttatggaaa ttgccttaaa
     4381 tctcaagaaa tccgatctgg cagttaaaat aatggaagat gccgttgaaa ataatcctga
     4441 tgaaccattg gcctattata atctgattaa cacctatgaa aagtgtaaga tgtttgagca
     4501 ggcggaagaa accagaaatc gtctcaagga tcgatttaac aagcgttcgc gagaaacggt
     4561 aaaaaagtga taccccaaca aggagaggta ccatgtttgg aagcttagga tttacagaat
     4621 taattctcat cctcatgatt gtgctgatca tttttggggc ggggaaattg ccgcaattgg
     4681 gcgaaggggt tggaaaagcg attaaaggtt tcaaaaaatc tgtccatgag gcagaggcgt
     4741 atgacctgga cccccaaacc caggccgaaa acacggcctc agccaaccat ggcaatcctc
     4801 aagcacaaat ttcacagcct atgcaaactg tccagactga aacggttccc ccaatccaaa
     4861 cggaggcagc acaccctgct ccacaagaac aggttcctgc ctcttctaaa gcctagtttt
     4921 gcttaaacgg caatggggca aaagtctttt tcgtagtggt cgtcgaacca gattgttttc
     4981 ggaataacca taaagggtaa atcttctatg agcatagaga ccgcgttgga aatcggggta
     5041 attgaaacct tcgccggcaa cggaaaatca cggagcacag gggatggcaa gcgagccgtg
     5101 aaatctggaa ttccgttgcc taaccatgtc accgttgatc gggatgggcg gtatttctac
     5161 attgcggaat ctgggtctga ccgaatcagg cgagtggaca tggagactgg gctgttgcat
     5221 aacttcgcgg ggactggtga gacatgttat agcggcgatt acgggccatg tggggaagct
     5281 ggtctctatc ttccattaga tgtcttgtgt gattcacaga atcatattta tgtctgtgat
     5341 tcaggtagca atcgaattcg aaaaattgac gcggattcgg gaattattac aaccatcgtt
     5401 ggtacggggc agtggggatt taatggtgat gggccagcat tggaatgcaa tttaacgtat
     5461 ccggcagcca tagccattga cgatcaggat catctttata ttgcagatac acaagcccat
     5521 cggattcggc attataatcc tcagacagga atgattacga caattgctgg gtgttggacg
     5581 gcagaagatg atgagcgtgt gagtcctttg acagcccaaa atcttattgt cctttccggc
     5641 gatgcgattg ggattgattt tagcaatgat gaaggtttat tgaggccgcg gtgttcagat
     5701 ggactggatt tatcgttata tcttgacgat ggcaagtctg ctttagagtg caaattgtac
     5761 gatgtggttg atatcgatgt ggaccggcat ggggatttgt acatcacgga taagggaaca
     5821 aaccgcattc gaaagctaga tcggaaaact ggcacgatgt ccaccgtggc tggaatctgc
     5881 cgctatgggt ttgacggtga tggaaagtta gcggtgaaat ccatgcttaa tgtccctgaa
     5941 ggcgctgtgg tagactcaga ggggaatgtc tatatttccg actcgatgaa tcaccgggtt
     6001 cgtaaagttg atagccagac cggtgtgatt actaccatcg cgggaaatgg ggatagtggc
     6061 tatgatgata agaatatggg tgggtgcggt gctgctcgct ttgtggcaaa agaagacgct
     6121 gggatgttga agcatggtga tggcctcatt gccgtagagg cgatagtgaa cactccagct
     6181 ggcttaacct tggactcaca ggggtatttg tatatttgtg aacgaaatga aaataaaatc
     6241 cgtcgattga aattacgata acgagattaa gttgattttt caatagaaat tgcctgcatt
     6301 cggtggagcc tttcttactc cagtgagata aataatttgc tgccccacgt actggttaaa
     6361 ggctatatca ttcgtttttc ttctctcccc ctgccctgtt ggcggagttc aatcatttcc
     6421 aagttgccat ttttggtcga atctcttgaa actttctttc cggtttgtac tccgtatcgc
     6481 tctgcattta gaatggggta atgcagttat tcgagaaaca cgcactcaat gggaaagcga
     6541 tcgtgtagaa tggatgtgtt cgagaatggt atttttgaat tattggtatt gaatctttag
     6601 gaattccaag ggaaccaagt attgagcaca attcacgcca aaaaacggat tgttttgttc
     6661 gtggtttttg ctgtggtggt tttattcgga ttgacttggt atcaagtccc ccttgtcagt
     6721 tcacagggca tggttgtcct ttcgcatttt tcaaaagggg aagtccctac tcatccttcc
     6781 gattccgcgt gggagtccgt ggctcctctc cctgttcctc tcagtggcca aatcattacc
     6841 cgaccggttt ggccggagcc cagtgcacga gctctctcca tccgatcaat tcataacgga
     6901 aaagacattg cgtttttact ggaatggcaa gatgccacaa ttaatgaatc gttgacgcct
     6961 ggggtcttca gggatggggt tgcattggct cttcctctgg gagatgcacc tgcctttttt
     7021 tgcatgggcc aactcgatca ttatgtgaat atttggcatt ggaaagcgga ttggcagagc
     7081 gacgtggatc ggcgtgccga gcgagccaaa gaatctaagc gtgagaaatc aggtcctcgt
     7141 cggtttgaag tgatcccgcg tcggccctca tcggtcgagg acctcgtagg agggggtttt
     7201 agcaccctaa cgagcaagga acagcaggga cgcattcaag ggcaagcgga gtggaaaaac
     7261 ggtttatggc gagtggtcat gaaacggccc ctgacctcca ctggagatga tttggacaat
     7321 gaagcgatgc tggttcctgg gcgaatgcaa gccatagcct ttgcggtttg gaatggagaa
     7381 aataaggagc ggaatgggca aaaatctgtt gcctcctgga tgcaattact cattgatcca
     7441 gttccactat cacaaaatgg gccaggagta atggaatcca cgcataggtc atcatgagaa
     7501 tcttagaaat gaaaaggatg agatctatag gtggaatgcg aattttctta agtattttgt
     7561 gcctgtggtt tggattgatt gccctccaag cagagacatt tggtcagctt ccaaatgaga
     7621 atatgaccga agaaaaagcc atgatattgg ccgaacaatt tggaattgat gtgggggaag
     7681 tagacgagga aatcaagaaa attcttggat tgactaaagc tgaaggggtt gtagtgtatg
     7741 ccgtcatagg agggtctcct gccgaattat caggaataaa ggtgaaagcg atcattaagg
     7801 aagttgataa gtatgaaatt aaaacccttg tggatttggg aacggccttg gaacagaccc
     7861 tccccacaaa gaattttact gtcgccacct atgaacctgc agatccagag aatcaagggg
     7921 ttaccggcgg gcttaatttc cattttgtac gaattcttca ggattaaacc atttgcatgg
     7981 aaaatctgtt tagcaaaaat tttcggttgt tgaaaattgt acccttgcac aaccgatgga
     8041 ttttaacggc cctattgggg attttctgtt tgtctggagt cgggcttggt caacagccta
     8101 gctcagttat tggtgaagga gatgaatcct ggattgagga aattgagcag gtttttatcc
     8161 cttctgaaca atgcaaacaa tgtcatgacc ggcattatga agagtggaag gggatgcgtg
     8221 agcagaccat ggatttgaag acctttggcc gggtggatgg agctcttctc catgggacag
     8281 ctctcacctc accggtgttc aaaactgtct tgggcctgtg gcttcaaacc gatcccgatc
     8341 aggaacaacg tactcgttgc ctttcttgtc atgctcccgc ggtgaccgtg ttcccacagc
     8401 acactgatcg gattattgat caagtgatga agggaggaaa gcggggtaaa attgaaggca
     8461 ttagctgtag tgcctgtcat ctcattacgg ggattcaaga aaatgcctat ggacatccca
     8521 ctttcaaaat tgaatcaggg gcgattcttt acggaccata cgccgaacca gatgagaatc
     8581 tcgtgcatcc ctctgcgcag gcggatattt atcgaggagc acattactgc gcatcctgcc
     8641 attttgataa agttaaggac gtcactcgac ctgatatccc tggggaaatt ctgaaaggca
     8701 cgatttgtca ggattgtcat atggagcgtt ctacggggag ttcaacctct caacgtggag
     8761 ccctcactcg tccaatcgga aggcattggt ttcaaggtat tgtgattcct gggattatgc
     8821 tgagtaatag gaatttacag gccgaatggt tttcaagggt ggatattgag gctaaaaagg
     8881 tcaatggaca ggttgaggga gaggtcctgc tacggaatgg tgccttgcct catacatttc
     8941 ccggtggcga tccagtattg aagcaatttt atgtcacgat tacgctcaaa aattcagatg
     9001 gacaggttat cgaccaatac caggaacggt ttggccgaac atttgaggaa ctgcttcgcg
     9061 gtccgatccc cagaccattt gtcaatggag gtacaaccag gcatattcca tttacattga
     9121 aagttcccca aggtacagag gttaccctga ttgaggctgc agtgagttat tctctcattc
     9181 ccgagccatc taaggctctt tccacaaaat tcatcgaagg cctggccacc gataaagaac
     9241 gggaaacggc agaacggatt attaaagatt attcttcccc gcggctcctc acgtttcgca
     9301 ccatgagttt atagacagtc aaaacttgca gattgtttgt tggaaaacat aaaaggattg
     9361 tctgatgacc aaaagctaca aagagagaac acccgggggt gaagttatga ggggggggat
     9421 attgttgttg ttgggatgtt ttctgctggc gatgcctcct caggcctctg tggcaagtga
     9481 atcgtcttta aacaaaaaac caatagaaaa atcatttccc aattctgaaa aatgtaaacg
     9541 ttgtcatctt cgagtctttg atgaatggga agcttcggct caatcgcgtt ctattgtgac
     9601 tgccgctttc cgcgtaaccc tggacaaatt tctcgcttca tccgataaaa aagaccatgc
     9661 aatgtgtttt cgttgtcacg ctccgcacat tcttgaatat ggcgaccatc tgcctcattt
     9721 tattaaagag gttcaatcaa aggatccacg gatggatgga gttggatgtc cgcaatgcca
     9781 tcttattcag gatgtagaca tgaattcgca tcctccaacg ccaacattcc aattgggaac
     9841 aacaattttt ggaggatatg ataaagccgc tgagaatttg gcacatcaat cacagaagct
     9901 ggaactctat cgagagtcta aattctgcgt gacctgtcac gattcccttc caaagatcac
     9961 ggactctgcc aaggacctcc ctggttggct aggatcttgg gaaaaaacta acgctgaaac
    10021 cagtgggaaa ccctgtcaaa cctgtcatat gccagaggca gttgatgaat ctgcaaatgg
    10081 agagaaggtc aggaaggtcg ccaatcacag cttccctggt aggttcggaa aggtccgtgc
    10141 ggatgctgtt accttggact tttcgactga ag
//
LOCUS       DAORAQ010000089        10169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1052_length_10169_cov_60.759245, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000089 DAORAQ010000000
VERSION     DAORAQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_10169_cov_60.759245"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1217
                     /gene="queA"
                     /locus_tag="PKM72_08925"
     CDS             195..1217
                     /gene="queA"
                     /locus_tag="PKM72_08925"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNP60949.1"
                     /translation="MDLSQFNFPFDQTLVAKYPVHPRDHARLLVLTRSTGDITDRQVK
                     DLPKTLRPGDLLVVNNTKVIPARLWAKKVPSGGRVELLIVKEIGEDHAEVLIQGKVGV
                     GQIVECEGSARAQIVEKEPGRTVIHWLGPGALRTWLLSHGEIPLPPYLKRPPRPGDQE
                     DYQTVFAKVEGAIAAPTAGLHFTPELITQLAEKGIGTAMVTLHVGPGTFQPVKTTDVE
                     RHIMHPEWFEVSEETARRIREVRNRGGRIVAVGTTVARSLESALDETGELRPCSGETR
                     LFILPGFQFRVIDALLTNFHFPETTLLMLVSAFAGLAEVKEAYAHAVRERYRFYSYGD
                     AMLIYE"
     gene            complement(1410..2111)
                     /locus_tag="PKM72_08930"
     CDS             complement(1410..2111)
                     /locus_tag="PKM72_08930"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNP60950.1"
                     /translation="MIRIIREGSAKYPWILKFLILGIAVTFVIGMGWYGYDSTQGNAV
                     ATVGPYKVSKDEYLRTKQGYFRFYRDQLKQEDVKEETIQQLALEGLITTKSWQLLADE
                     MNLAVSAQELHDAIVNQKDFQKDGVFDTEYYQRILAANRMKPHQYEEQRRNELLAEKA
                     RLLVSEATTLTPAEMKEVKELADRQTLDGAEPDLTVLEQIRLQFLMQKKQRAMQAFQT
                     ALRARGDVTIHQELL"
     gene            2448..3878
                     /gene="gltX"
                     /locus_tag="PKM72_08935"
     CDS             2448..3878
                     /gene="gltX"
                     /locus_tag="PKM72_08935"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNP60951.1"
                     /translation="MSEVRVRFAPSPTGFLHIGGVRTALFNWLFARHEGGTFILRIED
                     TDRDRSTDDAIQVILDGLKWTGLNWDEGPFRQTDRLEVYRERAMDLLKRKLAYWCICT
                     PEELDARRKEAQAKGESIKYDGRCRHRGISEPTEPAALRFLTPQEGQTVVNDRIKGRI
                     VFDNTVMDDLIILRSNGYPTYNFSVVVDDGLMNITHVIRGDDHVNNTPRQVPLFSAMG
                     FSVPEFAHLPMILGSDKARLSKRHGATSVLAYKEMGYLPEALINYLVRLGWSHGDQEI
                     FSIQEMVEFFDFKQVQASAAVFNPEKLRWVNAQYIRSSNPKQVADALMPFLQNAGYDD
                     AALARIPGGAEALIPPLRERSETLLDLVQGAVPYLSEPMTMDEDAAKKHLTLSIAPSL
                     QAFADQVEAESDFSKEALEGILHGIIERQGLKMGKVAQPLRVALTGRTVSPGIYEVMA
                     LLGKERSLQRIRAGVKRTMNPQTHPS"
     gene            3938..4011
                     /locus_tag="PKM72_08940"
     tRNA            3938..4011
                     /locus_tag="PKM72_08940"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3970..3972,aa:Gln,seq:ctg)
     assembly_gap    4446..4545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5496..5675
                     /locus_tag="PKM72_08945"
     CDS             5496..5675
                     /locus_tag="PKM72_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60952.1"
                     /translation="MLAGVIGLGFADMVELSFIRQWVVLMLFIQLGVVAHFSSQQNFI
                     EGRHAQIDPYSSNPY"
     gene            5872..7848
                     /locus_tag="PKM72_08950"
     CDS             5872..7848
                     /locus_tag="PKM72_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNP60953.1"
                     /translation="MVSCRENGSAANIQGTLVSTIGSIVTAFLIADLNPVYGCSLQRP
                     SVAFDGSNYLVVFKQVTETGRTDIVGARITPASAVLDGSGGFTILQDVVGAAVVAFDG
                     SNYLVVSDRYSDATLHDIIGARVNSDGHVLDEFPIFTAPGGQVLPSIAFDGNNYLVIW
                     SDTRSGSPIGTDANIYGTRVSPLGLVLDPGGIPISTAPGSQGFKDVIFDGENYFAVWD
                     DTRNGLGGFDLKIDIYGTRITPSGVLLDGSPDTGGIAISTHPRPQERPVVSFTGTEYF
                     VTWGMSYFLDPPEGIFAARVSTEGVLLDGPPGVGGILISNPKNNDSKFVSPNSYFNGS
                     NVLLSWINNRELGGTFKDLIGVLVSDTSHTPYDLNGDSNADILWRNTSDGNIAIWFMN
                     GMVTASFSFPGAVSLAWKIAGVGDVNADGKGDVIWHNQTSGAVAIWLMNGGTVTAVGF
                     PGTAPTTWSIQAVGDINGDGKADLIWRNTSEGHTAIWFMNGTAMASSRFPGGVPMAWQ
                     IAQVGDVDGDGKAEVIWRHSTSGAVAIWLMNDGTVTGVGFPGAAPTAWRIQAVGDVDG
                     DRRADLLWRNTRDGNTAIWFMNGTAVVAAAFRDGVPLVWQIEQAADVNGDSKADIIWR
                     NAIRGTVAIWLMDGLTVTAVGFPGSVSTGWKIQP"
     gene            8441..8716
                     /locus_tag="PKM72_08955"
     CDS             8441..8716
                     /locus_tag="PKM72_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60954.1"
                     /translation="MNLHQRGAELINGLKDHILEVLRGHPDAESEGMGVIQEEIARRA
                     GLHNMGTGELDHTCGEMLRLLQKEGLIQLVVEGEPDEKRKRWRLCSR"
     gene            complement(8789..8998)
                     /locus_tag="PKM72_08960"
     CDS             complement(8789..8998)
                     /locus_tag="PKM72_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60955.1"
                     /translation="MKRKAPRSGRGKYLPYGCLKTYARRKIQGWLQDIPEQEVPEYLG
                     REKSGSKTVADEQPGYRGDTPCWRP"
     gene            9285..9650
                     /locus_tag="PKM72_08965"
     CDS             9285..9650
                     /locus_tag="PKM72_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60956.1"
                     /translation="MKIVAPLFVMFWIILSSDPLLQAEVIIEADGGEEEELTLKDRKG
                     AKAPMRIEDPDGTLRPLPLGSIPESRMSKEGLRYVPDAGGGTMVNVEGRFQSILKVPD
                     GADHAIHHHESVDLQSLKP"
     gene            9679..>10169
                     /locus_tag="PKM72_08970"
     CDS             9679..>10169
                     /locus_tag="PKM72_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60957.1"
                     /translation="MNDPNANMLRGPSQLPISFSLCVAGFLVALLFGGLGLLAETDAA
                     TIVIVNKDSPGEGFNDPAPFTPVGGNTATTLGQARLKAFEYAANILAQGLVSTVGIRV
                     DARIDSLGAGILGSAGPNTVHRNFPNAPVANTWYVQSLANKFAEQDLDLTTSDISATF
                     SAN"
BASE COUNT         2315 a         2247 c         2796 g         2711 t
ORIGIN      
        1 aagtggtcgt ggtgggtcat ggggcgggac atgccgtggg gatgtgtcag tggggcatgc
       61 gggaaatggc cgaattaggg tatgactacc aatcgattat ccggcattat tatccccaca
      121 ccatacttct tcctcttgat cgcgtgctcc ttactgcggc cgactaactc tatcgtttct
      181 ttcctttcct ggtcatggat ctttcccaat tcaactttcc ctttgaccag accctggttg
      241 ctaaatatcc ggttcatccc cgggatcatg cccgattgtt ggtgctgacc cgatcaaccg
      301 gagatatcac cgatcggcaa gtcaaagacc tcccaaaaac attacgaccc ggggatttac
      361 tcgtggtcaa taataccaaa gtcattccgg cacgcctctg ggcaaagaag gtaccaagtg
      421 gagggcgggt agaattactg attgtcaaag aaattggtga ggaccatgca gaagtcctta
      481 ttcaggggaa agttggagtc ggacagattg tggaatgtga gggttctgcc cgtgcgcaga
      541 ttgtggaaaa agaaccaggt cgaacggtga ttcattggct gggtcctggt gccttgcgaa
      601 catggttact ttctcatgga gaaattccgc ttcctccgta tttgaaacgt ccgcctcgac
      661 caggcgatca agaagattat caaaccgtat ttgccaaggt ggagggggcc attgctgcgc
      721 caactgccgg attgcatttt acgccagaat tgatcacgca actggcagag aaggggatcg
      781 gaacagccat ggttaccctg catgttggac ctggaacctt tcagcccgtc aagacgacgg
      841 atgtggagcg tcatatcatg catccggaat ggttcgaggt gtcggaggag accgcgagga
      901 gaattcgcga ggttcggaac cggggcggga gaattgtcgc tgttgggact accgtggcac
      961 gaagtctgga gtcagccctg gatgagaccg gcgagctcag gccctgttca ggagagacgc
     1021 gcctctttat cctgccagga ttccaattcc gggtcattga tgcgcttctg accaattttc
     1081 attttcccga aacgaccctt ttaatgctgg tttcggcgtt tgcgggattg gcggaggtca
     1141 aggaggcgta tgcgcatgcg gtacgcgagc gctatcggtt ctacagttac ggcgacgcca
     1201 tgctcattta tgaataatga agttggaaga aatagtgagg taatccaggg gtgattttcc
     1261 cagcaggtgt tatctgacgt atggggcgaa ggatttgtgg taaggacgaa tgagtcgggg
     1321 gcaggtatct gcggataaaa tcccgagctg agccactggg ggaggactcc aggcagacca
     1381 gattgcaggg taaggccgag ggctgcctct tacaacagtt cctggtgaat tgtgacatcc
     1441 ccacgagctc gaagcgcagt ttggaatgcc tgcatggccc gttgtttctt ttgcatgaga
     1501 aattgaaggc gaatctgttc caggactgtg agatcgggct cggccccgtc aagtgtttgt
     1561 cgatcagcga gttctttgac ttctttcatt tcggccgggg tgagtgttgt ggcttcagag
     1621 acgagcagtc tcgccttttc cgccagtaat tcgtttcggc gttgctcctc atattgatga
     1681 ggtttcatcc ggttggccgc caagattctt tggtaatatt ctgtatcgaa gacgccatct
     1741 ttttggaagt ctttttgatt cacaatcgca tcgtggagtt cttgagccga gacagccaaa
     1801 ttcatttcgt ccgccagtaa ttgccaggat tttgtggtga ttaatccctc gagagcgagt
     1861 tgctgtatgg tctcttcttt tacatcttct tgtttaagtt ggtcccgata gaatcggaag
     1921 taaccttgtt ttgtccggag atattcgtcc ttggagacct tataggggcc aacggttgcc
     1981 acggcgttac cttgagtgga atcgtaccca taccaaccca tgccaatgac aaaagtgaca
     2041 gcgattccca ggatgagaaa cttgagaatc caggggtatt ttgcggaacc ctcgcgtata
     2101 attcgaatca tgcgtcctgc ctcctgagaa tgtctttggt gaccatgacg aaattttaag
     2161 ggccggcaac gggaaaggca agtgagtggg ccgtgtcaat tcagttcttt acatgagtcg
     2221 ggtggcatgg taaagcaacg ggccggagag tgagaatgat agatatttct taaatggtcg
     2281 tccgggatag agtgtgagga tttcctggaa ttgctctgtc cttgattttc attggaaagc
     2341 ggcgtggaga acggaacatg gcgtctctgc aaaacaatgt ttctgatggg ggagttgttt
     2401 cctataatat gtgcctgagt cataatgaga aaggtcggtg aaagcaaatg agtgaggttc
     2461 gagttcgctt tgcgcccagt ccgactggtt tcctccatat tggcggagtg cggacagcat
     2521 tgtttaattg gttattcgct cgtcatgagg gtggcacctt tatcctccgt attgaggaca
     2581 ccgatcggga ccggtctacg gacgacgcta ttcaggtcat cctggatggc ctgaaatgga
     2641 ccgggttgaa ttgggatgag gggcctttcc ggcagacaga ccgcttggag gtgtatcgtg
     2701 aacgggcgat ggatctcttg aagagaaagt tagcctactg gtgcatctgt acgcctgagg
     2761 aattagacgc gagaaggaag gaagctcagg ctaaaggcga atccatcaaa tatgacgggc
     2821 ggtgtcgaca tcgaggaatc tccgaaccca ccgagccggc ggctcttcga tttctcactc
     2881 ctcaggaagg gcaaaccgtc gtcaatgatc gaattaaggg tcggatcgtg tttgataata
     2941 cggttatgga cgacctaatc atactcagat ccaatggcta tcccacctac aatttttctg
     3001 tggtagtgga tgatgggctc atgaatatta cgcatgtcat tcggggggac gatcatgtca
     3061 ataatactcc gagacaagta ccgctctttt ccgccatggg attttcggtg cctgagtttg
     3121 cacatctacc catgatattg ggatcggata aagcgcgtct gtctaaacgc catggggcca
     3181 cctccgtttt agcatataag gagatggggt atttgcctga agccctcatt aattatttgg
     3241 tccgattggg atggtcccat ggggatcagg aaattttctc aattcaggaa atggtcgagt
     3301 ttttcgattt caagcaggtg caagcctcgg cagccgtttt taatcctgaa aaactacggt
     3361 gggtgaatgc gcagtacatt agaagcagca accccaagca ggtggccgac gccctcatgc
     3421 catttttgca aaatgcaggc tatgacgatg ctgctttggc cagaattccc gggggagccg
     3481 aggccttgat tccacccttg cgagagcgat cagaaaccct tttagacctt gttcaaggtg
     3541 ccgttcccta tttatcagag cccatgacga tggatgagga tgccgcgaaa aaacatctca
     3601 ccctgtcaat tgccccgagc cttcaggcgt ttgctgatca ggttgaggcg gagtcagact
     3661 tttccaagga ggcgcttgag ggtattcttc atggcattat cgagcggcag gggctcaaaa
     3721 tgggcaaggt cgcgcaaccg ttacgcgtgg ctttaaccgg taggactgtc agccctggca
     3781 tttatgaggt aatggcgtta ttagggaaag aaaggtcgct ccaacgaatc cgtgccggtg
     3841 ttaagcgaac gatgaatccc caaacccatc cttcataaat tgtttttatg cagtcccttg
     3901 actggttccg agaggtgaat taagctcact cctaggttgg gggatcgtct agcggtagga
     3961 cgccagtttc tggagctggc tgcccaggtt cgatccctgg tcccccagcc aactttttcc
     4021 ctcttctgaa ttataatctt caaactcttt cttcaactgc ttcatttttc ccttgggttt
     4081 attctgggaa tataagcgac ctgtagcctg tcgaatttgc gctatgccga aggtggcggt
     4141 tcgactcggc ttccttccta gttcaaacca gccctcgttg tttaagagct ttgttgacgg
     4201 ttcctgctga gagatggccc catctcatat ccttttgggc tctaatggtc tggaattcct
     4261 gaaattgcta gaaaagctcc agcccgacta ttactccccg actggctgtg gtatgccgga
     4321 gggtatgcca ctagcctcag gtgatgaatg tgatttctta ggtgtatttg tgccgtgtct
     4381 caaaacgagc ctgactctac acgggggcag ggtttgatgt ttcatccagc atcaggatct
     4441 tccctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatga ggattttgtc
     4561 tcgtccgaaa tacttatgcc taggccgtgg agggagtgtt gatcattttt tgccgaaata
     4621 gtgggatttt ttaaggtcat ataagttcat catgaaatca cgaacaggtt ttgtcatttt
     4681 tgtgaccagt gtgtccttaa cgttaagtat gctcaacata aaacctgcgc gggctgcgta
     4741 gtttatgggg ttggggaaat tctcggtgaa ggttttttag ccaagctttg gctgtttcta
     4801 ccgaaggatt tgtagtcgtg gacatggggc tggcttttcg gggcacaacc agtggctttg
     4861 aacccattgt cgatttgaca gggatgatga ctcttacggc taatggagta actagtgagg
     4921 gccgttttat aatttaataa tcgaaggata atcccattag ggattagggc tgacagcaca
     4981 agcttggaga tggacaggag gtagcggcgt tgttggcttg gggaatctct atgggggatt
     5041 tagaagtaat gttacggcca tctcgttcga tggattagtg atcgtgggca atagcacagg
     5101 cggaagcagg cgaggaggca ttccggtagg tcaaggagcg gcatggtcga gtttggattc
     5161 ctgcctggag caatatttaa tacaaataca tgggccattt ctgatgattg atcagtaata
     5221 cttggaagca gctgcttact tctgggggcg aagggttaat gcgtgggaaa aattaggatg
     5281 cccttttttt ttaatacagg gtgtgcatat gtgggatttc aagctggaac gatccacgtt
     5341 tttgccccat tctcacaatc accatattga aacattggcc gagtagagtc ttatcggtct
     5401 gaatgccctc tcattcccct tggcttgcgc actgctaatg ggttggcgag cgtacagagc
     5461 tgaagacaac gaaataccca atctataagg ccgggatgct tgctggcgta attgggttag
     5521 gttttgcaga tatggttgaa cttagcttta tccgccagtg ggtagttctc atgctgttca
     5581 tccagctagg ggttgttgct catttttcca gtcagcaaaa tttcatagag gggcgtcatg
     5641 ctcaaattga tccctattcc tcaaatcctt actaggtggg ccaccatttc tttggcctgt
     5701 gcatgtttgg ttttgggggt gtggcctcag tgggccactc aattgcaagc acaaaccggc
     5761 tataggtaca tatcacccgc gttcataatc gatagcggag tcaccccggc gaactcagat
     5821 acaggaagtc ccggcaagcc aggtataggt tttgacggag tcaattatct aatggtgtcc
     5881 tgccgagaga atggctccgc cgccaatatc caggggacac tcgtctctac gataggctca
     5941 atcgtaacgg cttttctgat tgccgacctt aatccagttt atggttgctc cctacaacga
     6001 ccctctgtgg cttttgacgg aagtaactat ctggttgtct tcaagcaagt aaccgaaacg
     6061 gggcgtacgg acattgtggg ggctagaatt accccggcca gtgccgtact tgacgggtca
     6121 gggggattca cgatcctcca agatgtggtg ggagctgccg ttgttgcatt cgacggttcc
     6181 aactatcttg tcgtgtcgga tagatatagt gatgccaccc ttcacgacat cattggagcc
     6241 agagtgaatt ctgacggaca tgtgttagat gaatttccga tatttaccgc cccgggtggt
     6301 caagtcttac cttcaattgc atttgatggc aacaactacc tcgtcatttg gtcggataca
     6361 agaagtggat cacccatagg aacggatgcc aatatttatg gcacccgggt gtctccgctg
     6421 ggcctggtgt tggatccagg gggaatcccg atctctactg ctccgggtag tcaaggattt
     6481 aaggatgtca tttttgacgg agagaattat tttgccgtct gggatgacac acgcaatggt
     6541 ctaggcggtt ttgatctcaa aatagacatt tatggtacgc gcataacccc ttccggtgta
     6601 ttactcgatg gatcaccgga cacagggggg attgctatta gcactcatcc ccgccctcag
     6661 gagcgtcctg tggtcagttt cactggcaca gaatattttg tcacttgggg gatgtcatat
     6721 tttctagatc ccccagaagg aatatttgca gcgagggtct ctactgaggg tgttctcctt
     6781 gatgggccgc ctggtgttgg agggatactg ataagtaatc cgaaaaacaa cgactccaaa
     6841 ttcgtttcgc ctaatagtta cttcaatggg agcaatgtgc ttctgagttg gattaataac
     6901 agagaattag gtgggacctt caaggatctc atcggtgttt tggtatcaga tacatcacat
     6961 accccctatg acctgaatgg ggacagcaat gccgatattc tctggcgtaa caccagtgat
     7021 ggcaacattg ccatctggtt catgaatggg atggtcacgg cctctttcag ctttcccggt
     7081 gcggtatcct tggcttggaa aatcgcgggg gttggcgatg tgaatgcaga tggcaaaggg
     7141 gatgtcatct ggcacaatca gaccagtggc gcggttgcca tctggctgat gaacggcggg
     7201 accgtgacag cggtgggctt tcccggtacc gccccgacga cttggagcat tcaggccgtt
     7261 ggggatatca acggcgatgg caaggcggac ctgatctggc gcaacaccag tgagggtcat
     7321 acggccatct ggtttatgaa tgggaccgcc atggcttcct ccaggtttcc gggcggggtg
     7381 ccaatggcct ggcagatcgc acaagtgggc gatgtggatg gcgatggcaa agcggaagtc
     7441 atttggcgcc atagcaccag tggtgcagtg gcgatctggc tgatgaatga cgggactgtg
     7501 acaggggtgg gctttcccgg cgccgccccg acggcgtgga ggattcaggc ggtcggcgat
     7561 gtggacggtg ataggagggc ggatctgttg tggcgcaaca ccagggatgg taacactgcc
     7621 atttggttca tgaatgggac tgctgtggtt gccgcagcct ttcgagacgg tgtgccctta
     7681 gtgtggcaga ttgaacaggc ggccgatgtg aatggggata gcaaggccga catcatttgg
     7741 cgcaatgcca tccgcggcac agtggccatc tggttgatgg atgggctaac tgtgacggcg
     7801 gtaggttttc ccggcagtgt gtcgacgggg tggaagatcc aaccgtaggc ggaggcccca
     7861 ctcaatttcc cgcattgagg tggcgtctta cggagttggg ctttttttgt gatggaaagc
     7921 ctgaataagg tccaggtgat tgaccgaaaa aagtcctctc tttcccaggt ctattttcta
     7981 catcttctgc ttaccctcag ttatctccag gtttagctcc agattctgaa tcgatgtaag
     8041 gtaaggatga taggtggtat gcaaaaagtg taaatatttt gtgttaatga tgcggctggc
     8101 actccgtcac catggatatt cgttccccgc cttttgatta tgtagatttt cggaactggc
     8161 tgcccaggtt cgatctttgg ttttccagtc aaaaactttc cacttttctc ctaaattaac
     8221 caacaatctt cgtcagacct gaaatctaca aaggaattct tagatttcct tttggcctca
     8281 aaaacgggtt gccggtccgt attgttgaat tggttgtata atcctgagaa gattaaagcg
     8341 caaagggatg attaactcag aatggagttg gagggtgtgg cgtcggatct aacggaccca
     8401 atatcacatt tcaaagaatt accatttagg aggggacgct atgaatcttc atcaacgggg
     8461 cgccgagctg attaatggat taaaggatca tattctagag gttcttcgtg ggcatccgga
     8521 tgcggagtcc gagggaatgg gcgttattca ggaagaaatt gcccggcggg cagggttgca
     8581 taatatgggc accggggaat tggatcacac ctgtggggaa atgttgcggt tgcttcagaa
     8641 ggaaggccta atccaactgg tggtagaggg tgagccggat gaaaagagga agcggtggcg
     8701 tttgtgctca cgataagtta tgactcgttt ctccaatttt gcacttcaat tttgaattca
     8761 tttctctccg actgaaaatc gttgctcatc aagggcgcca acaaggggta tccccgcgat
     8821 agccgggctg ctcgtcggcc acggtcttgc ttccgctctt ctcccgccct aagtattccg
     8881 ggacctcctg ttccggaata tcttgaagcc agccttgaat cttccgacgg gcataagtct
     8941 tcagacagcc gtaaggcagg tacttgcctc tcccgctccg tggtgctttc cttttcattg
     9001 gcctatcctt actccgtcgg tccgggcaaa cagggtgtgg gggaaccttc acccgaaagg
     9061 ctatgcctcc cttcaccatt attgacagac ttttaattgt agctcgttgg ccggaaaata
     9121 tgacatactt ttttaaggaa acaccatgga tttccttttt tagttgtcat agccgaaacg
     9181 gaatattaga ttaaatagaa atgccagaat gattttcttt gatagggttc tctctgaatt
     9241 tttcctcaaa gaaaaaaggg aagttgttga atggaggaaa tgaaatgaaa attgtggcgc
     9301 cactatttgt catgttttgg attatactga gtagtgaccc tctcctgcaa gcagaggtca
     9361 ttattgaagc agatggtgga gaggaggaag aattgactct taaggatcgt aagggagcta
     9421 aagcacccat gcggattgaa gaccccgatg gaacgctacg accccttcca ttgggctcga
     9481 ttcccgagag tcgtatgagt aaggagggtt tgcggtatgt tccagatgca ggcggtggaa
     9541 caatggtcaa tgttgaaggc cggtttcagt caattttaaa ggtgccagat ggcgctgacc
     9601 atgcgattca tcaccatgaa tctgtggatt tacagtcctt gaaaccctga aaactttcca
     9661 aggctaccat ttcggttcat gaatgatcct aatgccaata tgctgagagg tccctctcag
     9721 ctgccgatat ccttcagcct gtgtgtggct gggtttctgg ttgccttact ctttggcggc
     9781 cttggcctct tggccgagac cgacgcagcc accattgtta ttgtgaataa agatagccct
     9841 ggtgagggat ttaacgatcc tgcccctttt accccagtgg gtgggaatac cgcgacaacg
     9901 cttgggcagg cgaggttgaa agcctttgag tatgcggcaa atattttagc gcagggattg
     9961 gtgagtacgg tgggcattcg agtcgatgct cgaattgatt ccttaggagc aggaatcctg
    10021 ggatcggctg gccctaatac cgtccatcgg aatttcccca atgctccggt ggcgaatacc
    10081 tggtatgttc aatccttggc aaataaattt gcagaacagg acctggatct gactaccagt
    10141 gatataagtg ccaccttcag tgcaaattt
//
LOCUS       DAORAQ010000090        10025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1072_length_10025_cov_68.339719, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000090 DAORAQ010000000
VERSION     DAORAQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1072_length_10025_cov_68.339719"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKM72_08975"
     CDS             complement(<1..687)
                     /locus_tag="PKM72_08975"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HNP60958.1"
                     /translation="MPLPHLKNTHVIVQHSSPDTKRILKSLLNAGLNACNPAQAIADT
                     LHLHKNRLQVQDIQYDLTHYDRIICVGAGKASAQMAMALEQVLGDKLEGGTIIVPDGY
                     GVPCDKVGIFEAAHPMPDERGVKATKQLLALTQSLSRRDLLIVLLSGGASSLLCAPSI
                     GLTLAAKRRTTQLLLRCGATIHDINIVRKHLSAIKGGQLAQASPATILTLIISDVLGD
                     DLSTIGSGPTV"
     gene            complement(899..3331)
                     /locus_tag="PKM72_08980"
     CDS             complement(899..3331)
                     /locus_tag="PKM72_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP60959.1"
                     /translation="MLTRPESSHIRPSDPPFSSSPLHQIIELRRKQIHQLFSLVPMAI
                     LASIINGGLVAFILMEVIPSTVVSWWIIGIVGINILWSGLVLAYRRVSKPLLHPSRWM
                     SLFVAGNSASGLIWGMAGIALYPSSSPSHQMFLALVLGGMAAGSTAIHAAYFPAFLAY
                     SLPTTLPLTYQFFAQGDPPHQAVGIMGFTFLAVITVTAYRNFSMIKNTLTLEKENFEL
                     ITQLKQAHSKAERFNRSLSKEIKNRIEIEKELLHHKDHLESLVGIRTADLQKSEARYR
                     MVVEKISDVIWVMDLEGSKFSYISPSLERLLGYSGHTLDTISPDTILMPASMVTLKEA
                     IKQERAHHESHPHEPNRSFLLVLEHQHHNGSSAWAEVRGSLLLDKHNRPIGITGVTRD
                     VTERKKTEEEKQRLEAQLLRSQKLESVGNLAGGIAHDFNNFLTTILGNITLTKQILPS
                     SKQPQAYLERAERAALHAKHLTHQLLTFSKGGDPIKQTVDLHELIKESSDLALSGSSL
                     LCQTWADPNLWTIEADPGQINQVLQNLLMNAIHAMPQGGSITIRAENLQLSDPDIRSP
                     LPLLTGPYVKVTITDEGIGIAEDHLPKIFEPYFTTKSAGQGLGLASTYAIMKKHAGHV
                     AVESQVGRGTTFTLYFPASPRATPSPKPLNLDIRQGQGKILVMDDEESIRMMAGEMLS
                     HCGYEVKMAKDGEEALALYQQAMDSHTPFLLVILDLTVPGKLGGMGTLARLRQLDPQV
                     KALVSSGYSNDPIMANYASHGFVGVITKPYSLIELSNMVHQRLWRQSPLASGQTPSDP
                     SGQMSSFFPSSH"
     gene            3620..4306
                     /locus_tag="PKM72_08985"
     CDS             3620..4306
                     /locus_tag="PKM72_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNP60960.1"
                     /translation="MKSFTLLQKTITDCTICERLTTYRQAIAKEKVKRFRDWNYWGRP
                     IAGFGDPKAQVFVVGLAPAAHGGNRTGRVFTGDRSGDWLYEALHAFGFANQPDSTHRD
                     DGLTLTNCFVAAVVRCAPPANKPTKAEFAACRPYLQEELRLLGRVKVIVALGKIAFDE
                     YLRTCQAMGLHVPSPRPKFSHGVQYALPWGVTLVGSYHPSQQNTFTGTLTRPMFHGIF
                     KTVRKLIAPS"
     gene            complement(4340..4993)
                     /gene="fsa"
                     /locus_tag="PKM72_08990"
     CDS             complement(4340..4993)
                     /gene="fsa"
                     /locus_tag="PKM72_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNP60961.1"
                     /translation="MKLYLDTGSVKEIREAASYGLVDGITTNPSLVSKEGRDFKDLLL
                     EICGIVDGPISAEVVSTEAAAMVKEGKELAKLHKNIVVKCPLIPEGLKATKQLSSEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDVSSNGMELIRQILTIYENYNFTTQV
                     LVASVRHPQHVVEAALTGGHICTMPYAVFQQLVKHPLTDNGLQKFLSDWEARPSNKA"
     gene            5330..5641
                     /locus_tag="PKM72_08995"
     CDS             5330..5641
                     /locus_tag="PKM72_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60962.1"
                     /translation="MIHKYLLIGLLGLLCAGCGGNPYLDASLDPAKFEGKDKAWFEDN
                     WGKPSGKSARFFGGETWVYSRISGGESRFPNFNFAPNLCRINLDFDKEGKLEDYDYTD
                     C"
     gene            5761..6522
                     /gene="pgl"
                     /locus_tag="PKM72_09000"
     CDS             5761..6522
                     /gene="pgl"
                     /locus_tag="PKM72_09000"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNP60963.1"
                     /translation="MSRTIIITRTIDELFVAAAREVVQVVLDCRNVGRECRVALAGGS
                     TPRGLFKLLTGAPYRAQISWDHLRVFWGDERTVPPDHKESNFRMAKEALLSHVPIPPN
                     QVFRIEGEFPADEAAARYETVLREQFNLSSGEVPSFDLILLGMGPDGHTASLFPGTSA
                     VAESQKLVAAPWVEKLQTHRVTLTPPVLNAAKHVVFLVSGVDKATALQVVLEGPVDPA
                     RYPAQVVNPTAGHLVWLINQDAAGLLKRETMTSRV"
     gene            6538..6933
                     /locus_tag="PKM72_09005"
     CDS             6538..6933
                     /locus_tag="PKM72_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60964.1"
                     /translation="MKMVKAPEAFIHALHKKPMTCPTRDCSGSVAVEERSRPTDRVKS
                     FGLRCEQCDWHDTITGDEQLDPPWDEGSLMEITEEHLLHLEPVCPYDQSPVDFHSLPN
                     PRRRARYRISCFFCGRQEELDWPPEEAKR"
     gene            7034..7789
                     /locus_tag="PKM72_09010"
     CDS             7034..7789
                     /locus_tag="PKM72_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60965.1"
                     /translation="MKKIKKKKSASQAVPAFLVGWTHGEPPPPGYIAAWFDEAYGGPL
                     RIRFLASTGHHHFEAAHTNWMAEVNIEPSTEILEQWQGRLQWEQTRLALLFPPSNTAT
                     DRRDVVLHVARMARGVTLLTDGTTYDVATGAYLNPSDWRDRDLEIFQITDHIQVEHRE
                     NVQRARMWFHTRGMTKFGLEEIETFQPMGLSGREVKDTVLRIARHLIEEGKNPKVGER
                     IVLEGSGPQVVVVRHRTDPVYGIPLAFREFTLE"
     gene            complement(7829..8371)
                     /locus_tag="PKM72_09015"
     CDS             complement(7829..8371)
                     /locus_tag="PKM72_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60966.1"
                     /translation="MKKFFLLGLISLIVLPTLAHADSYAPLADFSAVSKNPQCRLTTM
                     QEIFLHNWTPPAQYFMIPSYPNAVLASAMPSANAQIHGQAYQTIPSAVLLTPDPPEKI
                     LEFYRRSLDASWFQANDIGMIYLYRLPQPVASGEALTRQLMSKPGSIPNIAIDTELSP
                     CDQAIGGGARTRITVVSPPR"
     gene            complement(8602..9540)
                     /locus_tag="PKM72_09020"
     CDS             complement(8602..9540)
                     /locus_tag="PKM72_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNP60967.1"
                     /translation="MSGELLVTLVLAGLAFIILAKTAVVVPQQSAYVVERLGRYASTL
                     NAGLHILIPFLDVVRYKHTLKEMALDVPEQICITRDNVQVGVDGVLYIKVLDAERASY
                     GITDYRFAISQLAQTTLRSEIGKIDLDKTFEERTTINISVVNELDKASSPWGAKVLRY
                     EIRNINPPKDVLSAMEKQMRAEREKRAVVLASEGQRDAAINEAEGNKQQVIKASEAKR
                     QQQINEAEGEASAILAIATATAEGIRKVAEAIQLPGGYEAVQLRVAEQYIGEFGELAK
                     ASNTLVLPASVSDVGSMIALAMNVINQKPATAPKLS"
     gene            complement(9530..>10025)
                     /locus_tag="PKM72_09025"
     CDS             complement(9530..>10025)
                     /locus_tag="PKM72_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNP60968.1"
                     /translation="TERSPSAMLWWHWSIMGLVLIGLEILTLGGLGNFYFLFFGVAAL
                     MVSALTWLTLTEAAWLQWLCFVLFGILSLFAMRKPLQGTGFIREKDGVPVDSMVGEVA
                     TVLENLEAQRIGKVELHGTSWTARNAGTSTLGKGSQAKVIRVEGLTLWIQADAIAQEG
                     SHVG"
BASE COUNT         2381 a         2352 c         2760 g         2532 t
ORIGIN      
        1 gaccgtcggg ccggatccaa tggtggatag gtcatctcct aaaacatcgg atatgattaa
       61 ggtcaggatc gtcgccgggg aggcttgggc taattgcccg cccttgatgg cagacaaatg
      121 ttttcgaacg atattgatgt catgaatcgt ggctccgcat cgtaaaagca actgcgtggt
      181 acgtcgtttg gcagctaatg tgaggccgat tgatggcgca cagagcaggc ttgaggcccc
      241 accggaaagt agaacaatca acaagtcacg tcgagaaagt gactgagtca gtgccaggag
      301 ctgtttcgtg gcttttactc ctcgttcgtc cggcatcgga tgtgcggctt caaaaatgcc
      361 aaccttgtcg caaggtacac catatccgtc agggacaatg atggttccac cttccagctt
      421 atctcctagc acctgctcca gagccatcgc catctgtgca gaggctttcc cagctccgac
      481 gcagatgatg cgatcatagt gagtcagatc gtactgaata tcttgaacct gaagtcggtt
      541 cttgtggaga tggagggtat ctgcaatggc ttgagcaggg ttgcacgcat tcagtcctgc
      601 attgaggagg gatttcaaaa tgcgtttagt gtccggactg gaatgttgga cgatgacgtg
      661 tgtgttttta aggtgtggta atggcatctg gatgattgct caagacatga tggtaaggag
      721 agtaaatgat ttttcgaata aaattgtctg caatgtacca tattatcaat cgaaatgagt
      781 ctgagaaata taaggcgagt ttctgtcacc ctcattagtc agtacaggga gttctcaaga
      841 agagatagag gccagagggt ctttcaccga gtgtatgaag ttggggaccg ggacacaact
      901 aatgggagga agggaagaaa gaggacatct gtcctgatgg atcagaaggg gtttgtccgg
      961 atgcgagggg agattgacgc cagagtcttt ggtgaaccat gttactcagt tcgatcagcg
     1021 aatagggctt ggttatcacg ccgacgaatc catgggaggc ataattggcc atgatcggat
     1081 cattcgaata tccgctggac acgagtgcct tgacctgtgg gtcaagttgg cgtaaccggg
     1141 ccagggttcc catgccgccg agttttccgg gcacggtcag gtccaggatc accaagagaa
     1201 aaggcgtatg agagtccatg gcctgttgat agagggccag cgcttcctct ccatcctttg
     1261 ccattttcac ctcgtatcca caatgcgata acatttctcc tgccatcatt cgaattgact
     1321 cttcatcatc catgactaaa atttttcctt gcccctgacg aatgtccaga ttcaagggtt
     1381 tgggcgatgg ggtggccctg ggagaggcag ggaagtagag tgtgaaggtg gtgcctcttc
     1441 cgacctggga ttccacagcc acatgaccgg cgtgtttttt cataatggca taggttgagg
     1501 ccaaccccaa accctgtcca gctgactttg ttgtaaagta tggctcaaag atttttggca
     1561 aatgatcttc ggcgattccg atcccttcgt cggtgatggt gactttcaca tatgggccag
     1621 taagaagagg cagagggctc ctgatgtccg gatcggataa ttggaggttt tctgcacgaa
     1681 tggtgatgga tcctccctga ggcatcgcat ggatggcgtt catgaggaga ttttgtaaaa
     1741 cctggttgat ttgcccaggg tccgcttcaa tggtccatag gtttggatcg gcccatgttt
     1801 ggcataaaag cgatgatccg gaaagagcca gatcggaaga ttccttgatc aactcgtgta
     1861 agtccacggt ttgtttaatg ggatcgccgc ctttcgagaa cgtgagtaat tggtgcgtca
     1921 gatgtttggc atgaagggcc gcacgttcag cgcgttctaa gtaggcctgg ggttgcttgg
     1981 aagaaggcaa gatttgtttg gtcagagtaa tatttcccag gatggtcgtc agaaagttgt
     2041 tgaagtcatg ggcgatgccg ccggctagat ttcccaccga ttccaatttt tgggaacgaa
     2101 gcaattgggc ttccaggcgc tgcttttcct cttcagtttt tttgcgttcc gtcacatctc
     2161 ttgtgactcc cgtaatgcct atggggcgat tgtgtttatc gagcaggagg cttccccgca
     2221 cctccgccca ggctgaggac ccattgtgat gttgatgttc caggactaac aaaaacgatc
     2281 gatttggctc atgagggtgg gattcgtgat gggcacgttc ctgtttgatg gcttccttca
     2341 gagtgaccat ggaagcaggc atcaaaatgg tgtccggcga aattgtgtca agggtatggc
     2401 ccgaataccc caacaaacgt tcgagagagg gactgatata ggaaaacttc gacccttcca
     2461 ggtccatgac ccaaatgaca tcggaaattt tttcaacaac cattcgatac cgggcttcag
     2521 atttttgtaa atcggcagtc cgaatcccca caagtgactc aagatggtct ttgtgatgaa
     2581 ggagttcctt ttcgatttct atgcggttct taatttcctt gcttaaggaa cgattaaacc
     2641 tttcagcttt cgagtgagcc tgtttcaatt gggtaattaa ttcgaaattc tctttttcta
     2701 aggtgagtgt gttttttatc atggaaaagt ttcggtaggc ggtgaccgta atcacagcca
     2761 gaaaggtaaa acccataata cccacggctt ggtggggagg gtcaccctgc gcgaaaaatt
     2821 gataagtgag gggcaacgta gtgggaagac tgtaggccaa aaacgcaggg aaataggctg
     2881 catggatggc agttgagcct gcggccattc cccccagcac caaggctaaa aacatttggt
     2941 ggcttgggga agaagatggg tacagtgcaa tgccggccat gccccaaatc aatccggatg
     3001 cactattgcc ggccacaaac aagctcatcc atctgcttgg gtgtaagagt ggtttcgaga
     3061 cccggcgata ggccaggacc aaacctgacc agagaatgtt aattcccaca atgccgatga
     3121 tccaccagga tacgaccgtt gagggaataa cctccatgag aataaaggca accagcccgc
     3181 cgttaatgat ggaggccaga atggccatcg gcactaaaga aaatagttga tgaatttgtt
     3241 tacgacgaag ttcaatgatt tggtggaggg gtgaagaaga gaagggtgga tctgagggtc
     3301 gaatgtgact cgattccggc cgcgtgagca tgtacgctat tccaatattt tagaggtccc
     3361 gacataagcg atatcatggt atgactggtt cataccgtca acctgacgaa gaagcgatag
     3421 tcggaacgga atcagccccg cctctaggtg atagcctgcc tctcatgaag gacaatgtca
     3481 agacgaccgg gattgtctgg ataaagaaag gaacatagga aagccggcta tcgccggggt
     3541 gctattcgaa ccggaaagtt tggatcggga tctctggagt ttggaccggt tgcggccaat
     3601 ttatgctacg atccccagga tgaaatcgtt cacgcttctc cagaagacca ttacggactg
     3661 cacgatctgt gaacggctga cgacctaccg tcaggctatt gccaaggaaa aggtcaaacg
     3721 gtttcgggat tggaattact ggggtcggcc cattgccggg ttcggggatc ccaaggctca
     3781 ggtcttcgtt gtgggcttgg ctcccgcagc tcatggagga aatcgaacgg gacgcgtgtt
     3841 taccggtgat cgaagcggag actggttgta tgaagcgctg catgcgttcg ggtttgccaa
     3901 ccaaccggac tcgacccatc gagacgatgg gctcacgctc accaattgtt ttgtcgcggc
     3961 ggtggtacgt tgtgcccccc ctgccaacaa acccacaaaa gcggaattcg ccgcctgccg
     4021 tccctatttg caggaagaat tacgattgtt ggggcgggtc aaggttatcg tggcgcttgg
     4081 gaagattgcc tttgatgaat acttgcggac ctgccaggca atgggtttgc acgtaccatc
     4141 tcctcgacca aaattcagcc atggggttca atatgcctta ccctggggag tgaccttggt
     4201 cgggtcttat catcccagtc agcaaaatac ctttacgggc acactcaccc gcccgatgtt
     4261 tcacgggata tttaaaactg tccgcaaact gatcgcaccg tcctgagatt cggatttttc
     4321 catggccata atggtccgat taggccttgt tggagggtct ggcttcccaa tccgagagaa
     4381 atttttgtaa accattatct gtcaacggat gtttgacgag ctgctggaat acggcatagg
     4441 gcatcgtgca aatatgtccg ccggtgaggg ccgcttcgac cacatgttgg gggtgtcgga
     4501 cgctggcgac gagcacctgt gtggtgaaat tgtagttttc gtagatcgtc aggatttgtc
     4561 gaataagctc catgccattt gaactgacat cgtccagccg gccaataaat ggcgagacgc
     4621 accatgcccc ggctttggcg gccaacaagg cttgagtggg tgagaagcac aatgtgacgt
     4681 tgacacggat tccttcgctg gacaattgtt tcgtggcctt gagcccttcc gggatgaggg
     4741 gacatttcac cacgatattc ttgtgaagtt tcgctaattc ctttccttcc ttcaccatgg
     4801 ccgcggcttc cgtgctgacg acttctgcac tgatgggccc atccacaatt ccgcaaattt
     4861 ccaggagtag atccttgaaa tcacgtccct ccttagacac aagcgaaggg tttgtggtga
     4921 tgccatcgac caggccataa cttgccgctt cacgaatctc cttgacgctg ccggtatcga
     4981 gatagagctt catatcgagt cccctttttg gtttgtccac tggtgttgtc tgagttgtct
     5041 cgtgtcctat tgtaatccac acccctgact tgtacaaatt tccctatccc ttatttagaa
     5101 ggaaaattca tctgcgagcc tgcctgacag atggtcacgg aggtggaagt gccagcctct
     5161 aggagggcag ttacctattg ttgtactacc atgcggagag gaagaagatg gagtcctcct
     5221 ccaggatgat gagtaacatt gtttgactgg tagaggatcg aaggctagaa ttttcacaga
     5281 gtaaacagtg tgttcgcgtt ttcagtcatt gttgggggag gaaacgggtg tgatccacaa
     5341 atatttgctg ataggcctgt tgggattatt gtgtgccggt tgtggaggga atccgtattt
     5401 agacgcctcc cttgatcccg ctaaatttga aggaaaagac aaggcctggt ttgaggacaa
     5461 ctggggaaag ccgagcggga aatccgctcg attcttcgga ggagaaacct gggtgtattc
     5521 acgtatatcc ggaggtgagt cgcgatttcc caattttaat tttgccccga atctatgtcg
     5581 tattaattta gattttgata aagaaggcaa gttggaagat tatgattata cggattgcta
     5641 agaactcttg tgcccaattc acctgccagg attgaggaga gcgtcgcgct gaatattcca
     5701 ttgcaaacgg attttctttc tttgggctgt ctgcccgctc ttggtacgta ggaggctgaa
     5761 ttgtcgcgga caattatcat tacacgaaca atcgacgagt tatttgtggc tgccgccagg
     5821 gaagtggtgc aggtggtgct ggactgtcgg aacgtcggtc gcgaatgccg tgtggcattg
     5881 gcgggtgggt ccacaccccg aggcttgttc aagctcttga ccggtgcccc ctaccgggct
     5941 cagatttcct gggaccacct tcgtgttttc tggggagatg agcgcacagt ccccccggat
     6001 cacaaagaga gtaatttccg catggctaaa gaggcgttat tatctcatgt accgattccc
     6061 cctaatcagg tgtttcggat tgaaggagaa tttccggccg atgaagccgc tgcacgatat
     6121 gaaaccgtct tgcgggaaca attcaacctg tcatcgggag aggttccatc gttcgatctg
     6181 atcctattgg gtatggggcc tgatgggcat acggcgtcat tatttcctgg aacctcggct
     6241 gtggcagagt ctcaaaaact ggtggcggct ccatgggtcg aaaaattaca aacgcatcgg
     6301 gtgacgttga ccccaccggt actcaatgcg gcaaagcacg tggtttttct ggtcagtgga
     6361 gtggataaag caactgccct acaggtggtg ttggaagggc cggtagatcc tgcccgctat
     6421 cccgcccagg ttgtgaatcc tacggcaggt cacctggtgt ggttgatcaa tcaggatgcg
     6481 gcgggtctat tgaagagaga aactatgacg tcacgtgtgt gaagaaggag aggtaaaatg
     6541 aaaatggtca aggcgccaga ggcatttatt cacgccctcc ataaaaaacc catgacgtgt
     6601 cccacgcggg attgttcggg atccgtggct gtggaagagc gttcccggcc aactgaccgg
     6661 gtgaaatcgt ttggattgcg ctgtgagcaa tgtgattggc acgacacgat aacgggcgat
     6721 gaacaactgg atccaccgtg ggatgaaggg tccttgatgg aaattaccga ggagcatctt
     6781 ctgcatttag aaccggtttg tccgtatgat cagtctccgg ttgattttca ttcgctgcct
     6841 aatccacgtc gtcgggctcg ctatcgcatt tcatgttttt tctgtggacg gcaggaggaa
     6901 ttggattggc cgcctgagga agcgaagcga taattcttgg gagtgtgccg gggcctgaga
     6961 tttcccctca gcgtccatgc ttgttattcc tcataccctg tcatttgtat cagactcatc
     7021 tttggtcgaa ctcatgaaaa aaataaaaaa aaagaaatcg gcttctcaag ccgtgccggc
     7081 ttttcttgtg gggtggactc atggagagcc ccctccgcca ggctatatcg ctgcctggtt
     7141 cgatgaagca tatggtgggc cattgcgcat acgttttctc gcctcgaccg ggcaccatca
     7201 ttttgaggcg gcccatacca attggatggc cgaagtcaat attgaacctt caactgaaat
     7261 attggaacag tggcagggtc gcttacagtg ggaacaaacc cgcttggccc ttctgtttcc
     7321 tccgagcaat acggcgactg atcgtcggga tgtcgtcctg catgtggcga gaatggcgag
     7381 gggagtgact ctgctcaccg acggaacaac ctatgatgtg gcgactggtg cgtacctcaa
     7441 tccctcggat tggcgtgatc gggatttgga gatctttcaa atcaccgatc atattcaggt
     7501 ggaacatcgt gaaaacgtgc agcgggcgcg catgtggttc cataccaggg gcatgacgaa
     7561 atttggcttg gaggaaatcg agacctttca acccatgggc ttatcgggtc gagaggtgaa
     7621 ggacaccgtg cttaggattg cgcgccattt aatcgaggag gggaagaacc caaaggtggg
     7681 ggaacgaatc gttcttgagg gaagtggccc tcaggtggta gtggtgcgtc atcggaccga
     7741 tcccgtatat ggaattcctc tcgcattccg agagtttacc ctcgaataac agcaacctca
     7801 gcacacagga ttctggcatc gaatgcggtt accgtggggg agaaacgacg gtaatgcgag
     7861 tccgtgcgcc tcctccaatc gcttgatcgc aggggctcag ttccgtatca atggcaatat
     7921 tgggaatgga tcccggcttg ctcatgagtt gccgggtaag tgcctctccc gaagccacag
     7981 gctgtggtag gcgatacaga tagatcatgc cgatatcgtt cgcttgaaac cagcttgcat
     8041 ctaagcttct cctgtaaaat tccagaattt tttcaggcgg atcaggggtg aggagcacgg
     8101 ctgaaggaat cgtctgatag gcctgtccat gaatttgagc atttgcgctg ggcatggcac
     8161 tggccaggac cgcattcgga tatgagggaa tcatgaaata ttgtgctgga ggcgtccagt
     8221 tgtggagaaa tatttcctgc atggtggtga gccggcattg ggggtttttg ctcacggcgg
     8281 aaaagtcagc caggggggca taggagtctg catgggcgag cgtcggtaac acgatcagag
     8341 atatgagacc cagcaaaaaa aactttttca tgccttgact ccttgcatcc ttgggggttg
     8401 agaacatttg caagttcagt atacaaaaag agaatcatgg agtaagcatg atgggccgtc
     8461 aacggagagg ggttccatga attcggcggc ttgagtggtg agacagaatg aaatttccca
     8521 gaacctgtca gggaagctat tcagcctcat aataccaaat agccccatga ggtgaggccg
     8581 accactggtg ctggtcttca gttatgaaag ctttggggca gttgctggtt tttgattaat
     8641 cacgttcatg gccaacgcaa tcatcgagcc cacatcggac acggaagcgg ggagcacgag
     8701 ggtgttactg gctttcgcca attccccaaa ctctccgata tattgttccg caacccgaag
     8761 ttgaacggcc tcatatccgc cggggagttg aatcgcttcg gcgacctttc gaataccctc
     8821 agccgtggcg gtcgcaatcg ccaaaatggc cgatgcttct ccttccgctt cattgatttg
     8881 ttgctgccgc tttgcttctg aggctttgat gacttgctgc ttgttcccct cggcttcatt
     8941 aatggccgca tcccgttgac cttcggatgc cagtaccacc gcgcgctttt cccgttccgc
     9001 ccgcatttgt ttttccatgg cgctgaggac gtctttgggc gggttgatat tacgaatttc
     9061 gtatcgaaga actttggcgc cccaggggga ggaagctttg tctagttcgt tcacgacgga
     9121 aatattgatt gtcgtccgtt cttcaaaggt tttatccagg tcaattttcc cgatctcact
     9181 acgaagggtg gtttgggcca gttgagaaat ggcgaaccgg taatccgtaa taccatagga
     9241 agcccgttcg gcatccaaga ctttgatata caagaccccg tcaacaccga cctgcacgtt
     9301 atctctggtt atgcaaattt gttcgggaac atccaaggcc atttctttta aggtatgttt
     9361 gtatcgaacg acatccaaaa aagggataag aatgtgaagt ccggcgttta atgttgaggc
     9421 atagcgtcct aatcgttcaa cgacatatgc gctctgttgt ggaaccacga cagccgtttt
     9481 tgccagaatg ataaacgcga gcccggccaa aaccaatgtg accaacaatt cacccgacat
     9541 gagatccctc ctgtgcgatc gcgtccgcct ggatccagag cgtcaaacct tccactcgaa
     9601 taactttggc ctgcgatcct tttcctaggg ttgatgttcc ggcattgcgg gccgtccagc
     9661 ttgttccatg gagttccacc ttccctattc gttgagcctc aagattttcc agaacagtgg
     9721 caacctctcc gaccatagaa tccacgggaa ccccatcctt ttcccttatg aaacctgttc
     9781 cttgaagggg tttcctcatg gcaaagagag agaggatgcc gaagagcacg aaacacaacc
     9841 attgtagcca ggcggcttcg gtcaaggtta accaggtgag ggcactcacc attaaagccg
     9901 cgaccccgaa aaacagaaaa taaaaattgc cgagccctcc cagggtgagg atttccaacc
     9961 ctattaatac caaacccatt atcgaccaat gccaccaaag catcgcggaa ggactcctct
    10021 ctgtt
//
LOCUS       DAORAQ010000091         9958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1085_length_9958_cov_69.801474, whole genome shotgun sequence.
ACCESSION   DAORAQ010000091 DAORAQ010000000
VERSION     DAORAQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1085_length_9958_cov_69.801474"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..1341)
                     /locus_tag="PKM72_09030"
     CDS             complement(334..1341)
                     /locus_tag="PKM72_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60969.1"
                     /translation="MSDLVQKTPSFELAVRVVDNVRSRMRDLFNIPGQGNFYALSSQL
                     DERLTAYFIEGRRNIFNEEKNYGYILAQWGTYKPQNNLKVNAYVFELVKEGPTAIGKV
                     LGVYTNNHGDKTGIRIRWEDLAKIYDEHRLYELAKQYLDSSYSNGDERKAVELFMSQY
                     EERTGAAALEVTQEANKQKFMHASSQGIQLFRVGQYELALEEFNRALDITDWNDEVYL
                     VQFLKLRKWHTLLELGLKSEGEVRAKFHSRAQRIASEDGEIRDLFNAAFPKGPAHAVQ
                     ELYCCIFYYLQWEAASAVEKDSVKKCFDAHFSLATAKRTFGGTKEITKRCSELLVLLR
                     P"
     gene            complement(2685..3368)
                     /locus_tag="PKM72_09035"
     CDS             complement(2685..3368)
                     /locus_tag="PKM72_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNP60970.1"
                     /translation="MRMILLFLLLMIVVLGPQLWVWWVFRQYRQHREDLGGTGGELAR
                     HLLNRFGLQKVRVEPTPMGDHYNPETKVVGLNPKHYNGRSLTAAVIAAHEVGHALQDH
                     EGYSLLKDRTHLIKFAQKAEKAGSYLMLGIPVLAGVTRIPAVGLAVLVVAIGTMSVSA
                     MVHLITLPVEWNASFRRALPILREGGYLAPPDLHGAKRILTAAALTYVASSLFSLVNM
                     WRWIRLVRR"
     gene            complement(3488..4276)
                     /locus_tag="PKM72_09040"
     CDS             complement(3488..4276)
                     /locus_tag="PKM72_09040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP60971.1"
                     /translation="MNLNLNGKTALVTASSGGIGLEIARALAAEGAKVIINGRTQTSV
                     EQAMAEIQADLPHAETISLVADNGTAAGCNHTISQVPEVDILVNNLGIYEAVGFFEET
                     DQAWQEMFEINIMSGVRLARHYLQSMLGRGHGRIVFISSESGVSPAPEMAHYSATKTM
                     QLGIARSLAELTKGTEVTVNSVLPGPTRTESVEKFIRNIFPDLPPAEAGRRFMSENRP
                     TSLIGRLIDPKEIGEIVAFVCSSRASVINGSCIRAEGGLVRTVF"
     gene            complement(4350..5819)
                     /locus_tag="PKM72_09045"
     CDS             complement(4350..5819)
                     /locus_tag="PKM72_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNP60972.1"
                     /translation="MAKIEHLNFQNTYAGLPDVFHERVKPTPFPNPYLVSVNPAAAEL
                     LDIDPTEWTRPEFAEYFCGAKLLPGSDPIAMLYSGHQFGHYVPQLGDGRAIMLGEVRN
                     QKGERWELQLKGAGLTRFSRDGDGRAVMRSTIREYLCGEAMHGLGIPTTRSLCIVAGE
                     EVVWRETPEPGAMLLRMAPTHVRFGSFEVFYYRRQFEYLKTLADYVIEYHYPHLEEAE
                     NPYARLVHEVAVRTGHLVAQWQAVGWAHGVLNTDNMSILGLTMDYGPFGFMEGYDPTF
                     ICNHSDHHGRYSFQNQPDIGYWNIRALARALSPFLEEADVNATPAVYEKAMTGKYAEL
                     MRAKLGLVESHAGDDKLVTDLLNLMDSSRADYTTLFRTLGTVRQESPTVPSELRDQFL
                     HREPFDDWTARYRERLRAEKSDDAERSVRMDQVNPKYILRNHLAQRAITQAVQQKDYS
                     EIDRLLKLLSDPFTEQPGMEVYALPPPPGEPSIIVSCSS"
     gene            6198..6404
                     /locus_tag="PKM72_09050"
     CDS             6198..6404
                     /locus_tag="PKM72_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60973.1"
                     /translation="MIRICAWCRQEGKTGILGRNPGESGEMNEPESHGICHDHGLLLR
                     QAFTRNLAHRPLSTATLASSSASF"
     gene            complement(6795..6983)
                     /locus_tag="PKM72_09055"
     CDS             complement(6795..6983)
                     /locus_tag="PKM72_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60974.1"
                     /translation="MHLIEFFIGSDPLSIGIALVLVLSVLAYGWYEVRRTARLPHIGT
                     VLIKNRLKTTSKFPTPRR"
     gene            7472..7906
                     /locus_tag="PKM72_09060"
     CDS             7472..7906
                     /locus_tag="PKM72_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNP60975.1"
                     /translation="MLSLTVFAPLDLALVRGATPVSLAPADDTEETVVTINIKSREFN
                     PNSLSLTVGQKTRLVLKNLDTELHAFLPVGLLTDIHLNVSGSGAPQFSKEGLSRVLLP
                     TRGQTDIVFIPSHPGTFPYFCDLPGHVMRGTIVVHKNESMVD"
     gene            7991..9754
                     /locus_tag="PKM72_09065"
     CDS             7991..9754
                     /locus_tag="PKM72_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60976.1"
                     /translation="MNIVRHQPIMTDTLQPAADHRPGSPVPRMWSRLKWAGLLCCSSL
                     LAFTPVWAAEPAPVDDEALLTTVREFANAVGNGDRVAAGQRDFVCLHQMAQQQLFSDG
                     NFPDPSNPIYEWCEQRRQEAHQRVIQQHDRALDNVWPGLGKLVDFSDFERFYIAETLH
                     QQLAPSFFVMQAIAVQEPTTPFVIETVESGTLPHASFPSPDETGVLATPTAFLTTRVS
                     YPNALTAPIANAPGAEDWAVPYKKAQRVVKSVNVKWVVLSDLKRLGFPTDRAVLDLIL
                     DGPRGTTIPFVVDPGGFMPRSAEWFGLPESMETIQKGIQQAQAAPTRLESIMRLNRIL
                     MIGPTHIPTLEVFSDQLYKGFLDYGSRLNGLQLGDERLAQRFNELYWTVQSQTDRFDM
                     SLGMEMGGKAEPMPADFLYRMIPVMEALASLEPGDFNNRIRLSSTYRWTNDQMAALSA
                     PQELLSEVPADQDELRAQVLLELAWARIGKVAWNRHFDDPDIQKGYEAAQKAFELTKD
                     PLNKFTAVYAMAYSLAFHVPRDNQAMLGLLQQARDWFEKTPGSSPQSWAYMLHNDTLK
                     GLVETDPAFKSLLAAQLDPAK"
BASE COUNT         2420 a         2612 c         2431 g         2495 t
ORIGIN      
        1 ttccaaaggg ctcagatgaa ttggacggca aggtaggatg actcagaagg tcaatccaag
       61 gttacatatt acgatgtgtg gggtaggagc tttcaattaa taccggacca atcgaatcca
      121 tcgcctcatg ttcaccaggc taaaaagtga gaaggcgaca taggtgaggg cggcggctgg
      181 taggatataa ttcgcgccgt gcaaatccgg agatgccagg gagccgcctt catccagaat
      241 gaaaaggacc cgccgaaacg tggcatttca tttcacgggt tcgaaattgg aacgatcccc
      301 gattggcaag ttggttcggt accttttcac tcttcatggt cttaacagca ccaatagctc
      361 actgcaacgt tttgttattt ccttggtccc tccgaaagtt cttttggcag tcgcgaggct
      421 gaagtgagcg tcaaagcatt tttttactga atctttttca acggctgacg cggcttccca
      481 ttggagataa taaaaaatac agcagtacag ttcttgtact gcgtgcgccg gacctttggg
      541 gaacgccgca ttgaataagt ctcgaatctc gccatcttct gaagcaatcc tctgagccct
      601 cgaatgaaat tttgcccgaa cctccccttc acttttcagt ccaagctcga gcagggtatg
      661 ccattttcgt aactttaaaa attggaccaa ataaacctca tcattccaat cagtgatgtc
      721 aagagcccga ttgaattctt ccaatgccaa ttcgtattgg cctaccctga acaactgaat
      781 tccttgacta ctggcgtgca tgaacttctg tttatttgct tcctgggtca cttcaagtgc
      841 cgcagctccc gtgcgctctt catactgact catgaatagt tctacggctt ttctttcatc
      901 gccgttagaa taagaagaat caaggtattg ttttgcaagt tcgtacagcc gatgttcatc
      961 ataaattttg gcaaggtcct cccatctaat acgaatgccg gttttgtccc cgtggttgtt
     1021 cgtgtacaca ccgagcactt tccctatggc agtgggacct tctttgacga gctcaaagac
     1081 ataagcattg accttcagat tattttgcgg cttgtacgtg ccccattgag ccaagatgta
     1141 cccgtaattc ttttcttcat tgaaaatatt tcgtcttcct tcaatgaaat atgcggtgag
     1201 cctctcatcc aattgtgagc tcagtgcata aaaattgcct tgcccaggga tattgaataa
     1261 atctctcatt ctgcttcgaa cgttatccac gactcgaacg gcaagctcaa aggacggagt
     1321 tttttggaca aggtcactca acacaaattc catctcatct gagagcaact tgttgtctat
     1381 aagttcaaac agaaggcgga ttgcggccag ggactccggg tcatggaaaa tatcagtttc
     1441 ttctcttctg aagagatgca tattcttgta caggcattct ataagtacga ccgaagtttc
     1501 acgggtcatt cttggcataa acagatgact tttctgccat agctcattta actgtttcct
     1561 cttcctcaca cttgaacgat gggaatcacc atcttctcca aacccatctt ggccaaaccg
     1621 actccaagta ttaatgaagg attcgagaac cagctccggc gcttccctgg acggaaccct
     1681 gagagtgaag tatttccaga aaacgtccgg atgactcaac cgtttctgac ggagatattc
     1741 tcgctccgag gcatcatctc caacatacgt tttggaacat cctttacaca cctcaaagaa
     1801 taatatttga agaagttggc gtaagacctc cggcctctcc tggttttcac atagcgtttc
     1861 gatgtgctct ttgatgagga ggtacgttct gtcatctctg gtggtgaatt catggggaaa
     1921 agaaaagaat gcctcctcac cccagggtgg gaggtatatc cacggatatt caaagatatc
     1981 ttcgtaaacg ttagggtaaa aagtcctgaa tgcttccaga atcaggaaat ctgaaaaatg
     2041 aaccttttcc ttcaaaggag ggagactttg attgagacca tgcaagaaaa gctttctgca
     2101 ctctagggta ggaaacagct tcgataactg ggaggcatag attggagtga aaattttctc
     2161 aaaatattca attcttttct catcaatctc gagatttctc aacagtctgg ctattgagga
     2221 aacatggtca tccttgtctg agaaataggg gtcgcgatct atggattcca tttcttctgg
     2281 agacagagaa atcctgggcc ggacaattcc atcatcagat gaagaatctt caggcctatc
     2341 tgtattcaat atggcggtgt cctccatagg atcgtcattt aaaaccaata cggttgttga
     2401 acttcccgga aagcttcaca ggttggattg catcggatct actatttcgt cggtcccaca
     2461 taggtcgaaa ttgaattccg tcaacttaag accagccaca agaagcatac ggaatttcat
     2521 tatccatttc cccaggaatc tggcttccta atcaaaaatt aatagattcg cccgctttcc
     2581 tgactgtcga tgagtttgtt tattccggat gggatctaaa aggaaaggaa ggatatcata
     2641 ttgttgcatt ccgaagcgta tccgcaggac gtctccaagg aggtttaccg cctgacgagg
     2701 cgaatccaac gccacatatt caccaggctg aagagcgagg aagcgacata ggtgagggcg
     2761 gcggcggtaa gaatacgttt ggcgccatgc aaatccggag gtgccagata gccgccttct
     2821 ctgaggatcg gcagagctcg acggaaactg gcattccatt ccacgggtaa ggtgatgagg
     2881 tgcaccatgg cggacacgct catggtccca atggccacga ctaagacagc aagacccacg
     2941 gcaggaatcc gggtcactcc agccaggaca ggaatgccca acatcagata cgacccggcc
     3001 ttttcggctt tctgcgcaaa tttgatcagg tgggttcgat ctttcaaaag tgaataacct
     3061 tcatgatctt gcagagcatg ccccacttca tgcgccgcga tgactgccgc ggtgagggat
     3121 ctaccattgt aatgtttcgg gttcaaaccg acgactttgg tttcgggatt gtaatggtcc
     3181 cccatcggcg tgggttccac acgcaccttt tgcagaccaa accgattgag caaatgacgg
     3241 gctaattccc cgcccgttcc cccaagatcc tcacggtgtt gacgatattg gcggaagacc
     3301 caccacaccc acagttgggg tcccaacacg acaatcatga gcaggagaaa aagaagaatc
     3361 atgcgcatgg aattactcta tctcctcagc ccgctcctta ccaagcaagg gattgctcca
     3421 tttgttgttt tcctgattac attcaaggat tttccggaga ggtctttttg cttattggcc
     3481 tgttccatca aaagaccgtc cgcacgagac cgccttcggc ccggatgcac gatccattaa
     3541 tcactgaggc gcgggaactg cagacgaacg ccacgatctc tccgatttct ttggggtcaa
     3601 tcagacgacc aatcagggag gtcgggcggt tttctgacat aaaacggcgc ccggcttccg
     3661 ctggcggaag gtcaggaaat atgttccgga tgaacttctc aacactttcc gtgcgggtgg
     3721 ggccggggag cactgaattg accgtcacct ccgtcccttt ggtgagttcg gccagtgaac
     3781 gggcaatgcc taactgcatg gttttggttg cgctgtaatg cgccatttcg ggagccgggg
     3841 agacgcctga ttcgcttgaa atgaatacga tccgtccgtg accacgcccg agcatgctct
     3901 ggaggtaatg acgggcgaga cgcacgccgc tcatgatatt gatttcgaac atctcctgcc
     3961 acgcctgatc cgtttcctca aaaaacccga cagcctcgta gatgccgaga ttgttgacca
     4021 gaatatcaac ctcgggcact tgggagatgg tgtggttgca accggcggca gtgccgttgt
     4081 cagccacaag agagatcgtc tctgcatggg gaagatcggc ctggatctcc gccatcgctt
     4141 gctccacgct ggtctgggta cgaccgttga ttatcacttt agcgccctca gcggcgagag
     4201 cacgcgcgat ttccagccct atgccccctg aggacgccgt caccaacgcc gttttcccat
     4261 ttagattcag attcatgatg acactccctt cccatgacgg acgagtggaa ctccccgatc
     4321 aggttttcta ctgtttttcg ctaaattgtt tacgaagagc acgagacgat aatcgatggt
     4381 tcgccgggag gcggaggcag ggcatagact tccatgccgg gttgttcggt aaagggatca
     4441 ctcaggagtt tcaacaaccg gtctatttcc gaatagtcct tctgctgaac ggcttgggta
     4501 atggcccgtt gcgcgagatg gttccggagg atgtacttgg ggttcacctg atccattctg
     4561 actgagcgct ccgcatcatc gcttttttcg gctcgaagcc gttcccgata ccgtgcggtc
     4621 caatcatcaa agggttctcg gtggaggaac tggtcccgta gttcggaggg taccgtggga
     4681 ctctcctggc gaacggtacc caaggtacgg aacagggtcg tgtaatccgc ccggctggaa
     4741 tccataaggt tcaacagatc agtcacgagt ttgtcgtctc ccgcatgcga ttcgaccaga
     4801 ccgagtttag cccgcatcag ttcggcatat tttcccgtca tcgccttttc atagactgcc
     4861 ggagtggcat tgacgtcggc ttcttccaaa aacggtgata aagcccgtgc cagcgcccga
     4921 atattccaat agccgatgtc gggttggttc tggaaggaat accgcccatg atggtctgaa
     4981 tgattgcaga taaacgttgg atcatagcct tccatgaacc cgaaggggcc gtaatccatc
     5041 gtcaacccga gtatggacat gttatcggta ttcaggacac catgggccca ccccacggct
     5101 tgccattgag ccacgagatg tccggtgcga accgcgactt catgcaccag cctggcatag
     5161 ggattctctg cttcttccaa atgcggataa tgatattcga tgacatagtc tgccagcgtt
     5221 ttcagatatt cgaattgacg gcgatagtaa aacacctcaa aactgccaaa ccgcacatgg
     5281 gtcggcgcca tccggagcag catcgccccc ggttctggtg tttcccgcca gaccacttct
     5341 tccccggcca caatacataa actgcgggtt gtgggaatcc ccaacccatg catggcttcg
     5401 ccacagagat attcccgaat ggtcgaacgc atcactgcac gaccatcgcc atcccttgag
     5461 aatcgtgtca gccctgcgcc cttcaattgc aactcccacc gctccccttt ttgattgcgc
     5521 acctcgccca gcatgatggc ccgcccgtcg ccgagctgcg gcacgtaatg tccgaattga
     5581 tgccccgaat acaacatggc aatgggatcg ctcccaggca acagcttggc cccgcagaag
     5641 tattcggcaa actccggacg ggtccattcc gtgggatcaa tatcgagtaa ttcggctgct
     5701 gccggattca cgctcacaag gtaaggattt ggaaaaggcg ttggcttgac tcgctcatga
     5761 aacacgtcag gcaggcccgc ataggtgttc tgaaaattga gatgttctat tttagccatg
     5821 gcttcattat ccgtgaccta ggcgaggtcg agcaaccaca aacatccttt tatccatttg
     5881 agccatttga aaattcaccc ctgaccgcga aaacctaaag accactcggc atgaaatgcc
     5941 acattggtgc gagctccaaa cgtttggctc aactccgtat ctctttcgat tcaactgtgt
     6001 gtgttatatt ctcaacaacc attttttcat ttcagtggaa atcgaaaaac atcaatatat
     6061 acaatacatt acgactaatg cttttttagg ttcaattatt gcttttgtca gagtgcagaa
     6121 gtctcaaagc tgagaagctt atgattattt tgtgcgcaaa ttttttgtta gaccctcgaa
     6181 aaagaaaagg agatggcatg attcggatct gtgcatggtg tcgacaggaa ggcaaaactg
     6241 gaatacttgg aagaaacccg ggagagtccg gggagatgaa tgagccggaa tcccacggga
     6301 tctgccacga ccatggactc ctcctgcgtc aggctttcac gcgaaacctc gctcatcgcc
     6361 ctctttcaac cgccactctc gcctcctcat cagcctcctt ctaaccagcg ttcactcttc
     6421 ctgatgctga gacccgggtg gcaggatgcc agaagtgtgt tctccagccg gggaacagtt
     6481 atgcatcagg gctgcgcaca tgggagtgtt gaataataat aatttccaag ggcctattaa
     6541 gccacaggca cgttggatat cagaggcctc ggaggcggtt caaaaaagcc tgccctgagc
     6601 cttccgaaga gttcgtccag caaggccgca gttatttttg cccgcagagc gtacttccag
     6661 tacgtgagca cggaaaaatg gtgagaacgc cgctgccggc atttttcaac tgccagcaca
     6721 tgggaatctc aacagctgcg ctttgtcgga gtgccgtctt gcggacgggg atcgaggaaa
     6781 ccggggaaag ttgcttatcg gcgaggagtt gggaatttgc tggtcgtctt taatcgattt
     6841 ttgatcagga ccgtcccgat atgagggagg cgggcggttc gacggacctc ataccatcca
     6901 tacgccaaca cgctcaggac caacacgagc gctatgccta tcgaaagggg gtcggaaccg
     6961 ataaaaaact caatgagatg cacagtggat cgttctccag atgtattctc attttttcgg
     7021 tagttcccgc aggatcttga tattttctct caccttccga attttattcc ttttatacat
     7081 atttccactt acggctgtac caaatttttt ccagggattg cctaaccatt cattctgctc
     7141 gtttcgcgcc ggaatcgcta acctgagtgt taaataataa taatttccaa gggccaatta
     7201 acccacaggc acggtgcata tgaaagacct cggggcggtt caaaaaaacc tgcgctgagc
     7261 ctttcgaagg gttccttcag gcctgccctg agtcatgtca gacggaaggc cgcaggcatt
     7321 tttgcgcgca gagcgtactt cgagtacgtg agtgcggaaa aatggcgaga acgccgctgg
     7381 cggctttttt caacagcccc ctagccggta taatgccttc attatgatgt tccccctgcc
     7441 ctcgttcaga ttttttcagg cctgtcttct gatgctgagt ctcacggtgt ttgcacccct
     7501 agacttggcc ctagtgaggg gagccacgcc ggtcagtctc gctcctgccg atgataccga
     7561 agaaacagtt gtgactatca atattaaatc tcgggaattc aaccccaaca gcctttcact
     7621 caccgtgggg caaaaaaccc gcctggtcct caaaaatttg gatacggaac tgcacgcatt
     7681 tctgccggtc ggcctactga cagatatcca cttgaatgtc agcggaagtg gtgccccaca
     7741 gttttccaaa gaaggactca gccgcgtttt gcttcccacc cgaggccaaa cagacattgt
     7801 ctttatccca tctcatcccg gtacgttccc atatttttgc gatttaccgg gccatgtgat
     7861 gcggggtacc atcgtggtac acaagaacga gagtatggta gattagccac ctgggcatga
     7921 gggacattcc cctttctact cgtacacaaa atcattaacc aagcatgttt gtgaggagga
     7981 gtttggttcc atgaatattg ttcgccatca accaattatg acagataccc tgcaacctgc
     8041 ggcagatcat cgccccgggt cccctgttcc ccggatgtgg tctaggctca aatgggcagg
     8101 cctcctgtgc tgttcctcgc tattagcctt tactcctgtg tgggcggccg agccagcacc
     8161 tgtcgatgac gaggccctgc tcaccaccgt tcgggaattt gccaatgcgg taggaaacgg
     8221 cgatcgagtt gcagccggcc aacgggactt tgtgtgctta catcagatgg cccaacagca
     8281 gttgttctca gacgggaact ttccagaccc gtcaaaccct atctatgaat ggtgtgaaca
     8341 gcgtcggcag gaggcccacc aacgggtcat tcaacaacac gaccgggcat tggataatgt
     8401 gtggcctgga ctggggaaac tggtcgattt ttcagacttc gaacgattct atattgcgga
     8461 gactctccac caacaattgg ctccgtcgtt ttttgtcatg caggccattg cagttcagga
     8521 accaacgacg ccatttgtca ttgagactgt ggaatcgggt accttgcccc atgcctcgtt
     8581 tccttcgccg gatgaaaccg gagtcctcgc cacacccacc gcctttctga ccacaagagt
     8641 gtcctatccg aatgccttga cggcacccat tgccaatgcc ccgggagccg aggattgggc
     8701 agtgccttat aaaaaggcac agcgggtggt gaaatccgtc aacgtgaaat gggtcgtgct
     8761 ttcagatctt aagcgattgg gatttcccac ggaccgtgcc gtgctggatt tgattcttga
     8821 tggcccgcgt ggcacaacca ttccatttgt ggtggatcca ggtggattta tgcctcgctc
     8881 agcagaatgg tttggcctcc cggaaagcat ggagaccata caaaaaggga ttcagcaggc
     8941 acaggcagcc cccaccagac tcgaatccat catgaggttg aaccggattt tgatgattgg
     9001 tccaactcac atccctaccc tcgaagtttt ttcagatcaa ctgtacaaag ggtttctcga
     9061 ttatggaagt cgtttgaacg gccttcaatt gggtgacgag cgactggccc aacggtttaa
     9121 cgaactctat tggacggtgc aatcccaaac cgatcggttt gatatgtccc tgggaatgga
     9181 aatgggcggg aaagctgagc cgatgcctgc cgattttctc tatcgaatga ttcccgtgat
     9241 ggaggcctta gcctccctgg agcccggtga tttcaacaac cgtattcgcc tgtcctcgac
     9301 ctaccgatgg acgaacgacc aaatggcggc tctgtccgct ccacaggaat tattgagtga
     9361 agtcccagcc gaccaggacg aactccgcgc acaggtgctc ttggaattgg cgtgggccag
     9421 aattgggaaa gtggcctgga accggcactt tgacgatccc gacattcaga aaggctatga
     9481 ggcagctcaa aaagcgttcg agctcacgaa agatccgctc aacaaattta cggcagtcta
     9541 tgcgatggcc tatagcctgg cgtttcacgt acctcgtgac aatcaggcga tgttgggtct
     9601 actacaacaa gcccgtgact ggtttgaaaa aactcccggc tcaagccctc aaagctgggc
     9661 gtatatgttg cacaatgata cgctgaaggg gttagtcgag actgatcccg ccttcaaatc
     9721 attgctggcc gcacagctag atcctgcaaa ataggtgaga gcggcctcat gaaattttca
     9781 catgcggtgt cccggacaaa ctgacgacca caataggctg gagaatcgca ttgcctcaat
     9841 gaacgttctc cagccttatt ggttcacctt ccctcttctt ttcaacacgg tccctctctc
     9901 ccgggaacct cttctttaaa tcacttccca tggagctaga tttccgaaaa aatcccgc
//
LOCUS       DAORAQ010000092         9900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1095_length_9900_cov_70.192382, whole genome shotgun sequence.
ACCESSION   DAORAQ010000092 DAORAQ010000000
VERSION     DAORAQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_9900_cov_70.192382"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKM72_09070"
     CDS             <1..435
                     /locus_tag="PKM72_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60977.1"
                     /translation="QWNTKENCQCLMIVGNDSAATEMLKEALGEWGYEIVTARNGWEA
                     LVLAGRRPVDGMLVDMNMPIMDGRTMLSELRWLGYRMPVLMMLGESDERIQSQLLMEG
                     AQDFILKPPHLPSLQQTCRQVFSKYAVEEYATSYFPAGSNKK"
     gene            688..1122
                     /locus_tag="PKM72_09075"
     CDS             688..1122
                     /locus_tag="PKM72_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60978.1"
                     /translation="MGEVDTSPAVAAKVMEDLTALDVDPDKCQRLYKAALIQSSTGVT
                     YRMLSRILETGKVDIVHYACDLDAEGKPTTKWKIRRILEQAPERFDKEVEAIKKSMAD
                     ESETVKGAWVHDMTGLPDVPAQGKSLDEWSRSMTSEVKKEPT"
     gene            complement(1158..2447)
                     /locus_tag="PKM72_09080"
     CDS             complement(1158..2447)
                     /locus_tag="PKM72_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNP60979.1"
                     /translation="MPRLLLLLPTTTYRAEAFLEAARRVQVSVTIGTEQAPESLLVAS
                     ENVLLLDVRHPQAAARTVIEFVQHHPIDAVIGVDDVTAVTAAAIAEAVGLPHNSVASV
                     TAARNKRQMRELLSRQGIPVPSHKVFPLDGDSGAFAKEVVYPCVVKPLILSASCGVIR
                     ANDEQEFENAFRRVSTLLTNLNLAAVDEEARWILVEDFVPGIEVALEGLLTKGVFQPL
                     AMFDKPDPLDGPYFEETLYITPSRLSSDRQQQVVDCADRTAKALGLWEGPVHGEFRLN
                     ENGAWVIEMAARAIGGRCSRTLDFSAGISLEELILRHALRMPIPPLLRQEQAAGVMML
                     PIPYSGMLHEVRGQAEARAVPGIIELTITAEPGELLIPLPEGTRYLGFLLARGKTPED
                     VERSLREAHQRLTVVISASPPTTDRSPSSPHSKAMRF"
     gene            complement(2590..3288)
                     /locus_tag="PKM72_09085"
     CDS             complement(2590..3288)
                     /locus_tag="PKM72_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNP60980.1"
                     /translation="MEAELKTQGTKIQELLGLKVPAVAIAFRDTAPAGIPRVTTPAPA
                     GCGYWKLAAEGRTFYTEGSDHFGCPVGAHTHGVELPPQVATELNGLVKTMVDMQYLTM
                     KEIPMIPHREETFHVAVYAPLTQATFTPDVVLVRGTVKQLMLLAEATQAAGVPGGSAT
                     MGRPTCAVLPESLQSGHAATSFGCIGNRVYTGLGDDEGYYAIPGARVPDVVSKLAVIT
                     EANRQLEAFHRSRL"
     gene            complement(3590..5107)
                     /locus_tag="PKM72_09090"
     CDS             complement(3590..5107)
                     /locus_tag="PKM72_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HNP60981.1"
                     /translation="MEPVTEPTKERLLLRPPGAIMFVSCYELGHQPLGIAQPMGFLEQ
                     VGFSPVGLDIAVETIDEPTLRLAKFVGISVPMHTALRLGVRVAELIREKNPDCHICFY
                     GLYASINAEYLLQELADTVVGGEYEIPLRSLIEALDRDQTINHLEGVSSLNRVVAPYL
                     KRPYSTTSTTSQPIPSRTGLPMLDQYARLEENGTERLVGYVETSRGCLHLCLHCPIVP
                     VYEGRFFLVPEAVVLEDIRHQVHAGATHITFGDPDFLNGPGHSLSIVRRMHEEFPQLT
                     FDFTAKIEHLLKHRALMPDFEAQGCLFIISAVESFSDHVLEQLHKGHTGADIITALAI
                     LREAGITLRPSLVAFTPWTSLDDYLHMLDMVEDHDLIDATDPVQLSIRLLVPPGSALL
                     AKSDIQRFLGPLDQASFQYPWTHPDDRMDQLHQAVSAIVEASAKEEEDAMVTFDRIRA
                     NADKIAGWPVETSTRTRVSRPDRPKPPRLTESWFCCAEPTTQQFKPLLTKGHGEI"
     gene            5914..6945
                     /locus_tag="PKM72_09095"
     CDS             5914..6945
                     /locus_tag="PKM72_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP60982.1"
                     /translation="MNHLPSSPLLAKDLMDSEALPLCRGKFENDLALQFLSGQYCGWP
                     VVDSEQKILGIVSDARLFEAVSRLYSLDDLKVEDTMTPPIYVHEDESLDKVLDVMTHQ
                     HVQRMPVVRNHNLVGVISRARVLQHCLSSPSASSRSVPRCAWCEREQDSSAAMVSTKE
                     GRNLAWFLSIDQLKFSEIDLAHTYCPTCVKTLRAVNTTSGNLSSDKREIREIRPCLLV
                     VDDDPTVTGLLTHALQEWGYEVLVARNGWEGLAVVSRQTVDGILLDMDMPIMDGRTML
                     DELRWLGHQVPVLMMSGATDERALRQLLHEGAQGFFVKPFHLKSLKEACRKILQKNVS
                     QASSPSHVA"
     gene            complement(7032..8156)
                     /locus_tag="PKM72_09100"
     CDS             complement(7032..8156)
                     /locus_tag="PKM72_09100"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNP60983.1"
                     /translation="MIEQSQTPIDRQKENGAPIILECSASRSGQSPLTSDLRKTGLHP
                     DNWYPLARSRDLKKGKMLGVSFAGYPIVLVRTVTGMVYALEDRCAHRQVPLHCGVVHG
                     EHVQCGYHSWTFDQTGRCVGVPYLEKNQARPSGVRSYPCREAYGLVFVFPGSVGRVEE
                     VEFPKVSLAADSQYKTRYLDRQVGCHYSFMHENLMDMNHQFLHRRLMGGIRTVFLELR
                     EGPHAVEVDYTFSRIKGKQPLGEKLILGSTKPSADGPKHDLMTICTNYPYQTLKFWTA
                     GSTYPALNLWNVYVPIDPAQRINHTYGLMMIRKPSIPGLIHLLWPFIVWFTDGIFAQD
                     RWIVEEEQKAFDRQGADWNQEVFPVIQSLRSLLGREGIAL"
     gene            8520..>9900
                     /locus_tag="PKM72_09105"
     CDS             8520..>9900
                     /locus_tag="PKM72_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNP60984.1"
                     /translation="MKPPERSWLGTGSIGRALMVLTTVFILALFGILLYTITTIQNQR
                     LDSVTVDLAGRQRMLSQRLMNEILLTSQGIPADYRFTQQMLTQTLDALLVGGQAVINP
                     KNGEKVALTSPPSQEILQALGEQQTLLAEFMHRADTFLKTQSDHPRYSFELDGLLAVN
                     VRLIEAANKAVKLYSRNSQEKISTMIVWESLIGILVIPFGILMTRQVKLANQELEHEI
                     QERSRIETALRYRIEIENLVTNLSTQFISLETQDLDAGINRALEAIGTFGGVDRSYVF
                     VIVDDGKTMNNTHEWCQTGIEPQITRLQGLQMHDLPWFADRLTSGPFFQVSSVANLPL
                     EASAEKEVFERQQIQSLVIVPMVLRGKLFGFLGLDSVRHEKTWSEEDIRLLQMAGEIF
                     VNGFERQEVEENLRKSEAAKVEAFRQSDALKTALLSLVSHELRTPLTAIKASIAGLIE
                     LSRQDASKMQ"
BASE COUNT         2359 a         2399 c         2631 g         2511 t
ORIGIN      
        1 caatggaata caaaagaaaa ttgtcaatgt ttaatgattg tggggaatga ttctgcggca
       61 acggaaatgt tgaaagaggc gctgggtgaa tggggatacg aaatcgttac ggcaaggaat
      121 ggatgggaag ctttggttct ggcgggtcgg cgtccggtgg atggaatgct tgtagatatg
      181 aacatgccca ttatggatgg gaggaccatg cttagtgaat tacgctggtt ggggtatcgg
      241 atgccagtgc taatgatgtt aggtgagtcg gacgaacgaa tccaatctca attgttgatg
      301 gaaggcgccc aggacttcat tttaaagcca cctcatctcc catctctcca gcaaacgtgt
      361 agacaggtat tttcaaagta tgcagttgag gaatatgcca cttcttattt ccccgctggt
      421 tctaacaaga aatgaaatag gttggttggg aggctttttc ttttaaatct tccttgcccg
      481 gtgcccacca tatttgagtt tatccctccc cgttcgtacc ttgccaagta gatccggttt
      541 attcctttaa ttcgattttt cgcatcgcag gcagcctatc tgcagtcacc aattggcctt
      601 cgtcttgctc tatttcagaa ttgtctggta ggttcaagac tggtgttttg ccatcaggct
      661 taaacccttg caggaaggaa tacgactatg ggagaggttg atacgtcgcc agcggttgcc
      721 gccaaggtca tggaggatct cacggcgtta gatgtggatc cggacaaatg tcagcgtctc
      781 tataaggccg cgcttattca gtcgagtacg ggcgtgacct atcggatgtt gtccaggatc
      841 cttgagaccg gaaaggtgga tatcgtccac tatgcctgtg atttggatgc agaaggtaag
      901 ccgaccacga agtggaaaat caggcgtatt cttgagcaag cccccgaacg gttcgacaag
      961 gaggttgagg ccatcaagaa gtccatggcc gacgagagtg agaccgtgaa gggcgcatgg
     1021 gtgcacgata tgaccgggct ccctgacgtg ccggcacaag ggaagagcct ggatgaatgg
     1081 tcgcggagca tgacctcaga ggtgaagaag gagccaacct agagagcgct aggttggcgg
     1141 atcctggttg gttgcattca aaatctcatg gcttttgagt gaggtgatga tggtgatctg
     1201 tcggttgtcg gtggtgaggc gcttatgacg accgtcaatc gttggtgggc ctcacgaagc
     1261 gaacgctcca catcctccgg tgtcttgcca cgtgctaaaa ggaagcccaa gtagcgtgtg
     1321 ccttccggaa ggggaatgag taattctcca ggctctgccg ttatcgtcag ttcgataatt
     1381 cctggtaccg ccctggcttc agcctgccct ctcacttcat gcagcatccc gctatacgga
     1441 attggaagca tcatcacgcc tgccgcctgc tcttgccgga gaagaggagg aatcggcatc
     1501 cggagtgcat gacgaaggat cagttcttcg agcgatatcc ctgccgaaaa gtccagcgtt
     1561 ctggaacatc gcccgccaat ggctcgtgcc gccatctcaa taacccatgc cccgttttca
     1621 ttcaagcgga attccccatg caccggccct tcccatagtc cgagtgcctt tgccgttcga
     1681 tctgcgcagt ccacaacttg ttgttgtcga tcggaggaga gacgggaagg tgtgatatag
     1741 agtgtctctt caaaataggg tccatcgagc ggatcaggct tatcgaacat cgccaggggc
     1801 tggaagactc cctttgtgag caaaccttcc aacgccactt cgattcccgg cacaaaatct
     1861 tccacgagga tccaacgggc ctcctcgtct actgctgcca gattcaaatt ggtcaggagg
     1921 gtgctcacac gacggaatgc attctcaaac tcctgttcgt cattggcacg aattacccca
     1981 caactggccg aaagaatgag aggttttacg acgcagggat agaccacttc ctttgcgaaa
     2041 gctcctgaat caccgtcgag ggggaatacc ttatgggaag gaacaggaat gccctgtcgg
     2101 gacaataatt cccgcatctg ccgtttgttt ctggcggcgg tgacggacgc gacagagtta
     2161 tggggtaatc cgactgcttc cgcgatggca gcagcggtga cagcggtgac gtcgtcgact
     2221 ccaatcactg catcgatcgg gtgatgttgg acgaattcaa tcacggtcct ggcggctgcc
     2281 tgtggatggc gaacatcgag tagcaacacg ttctccgacg cgaccagaag gctttccgga
     2341 gcctgctctg tcccaatggt tacagatacc tgcacccgcc gggcggcttc gagaaacgcc
     2401 tctgctcgat aggtggtggt cggcaagagc aggaggagtc ttggcatcgt gacttcttaa
     2461 caggagggtg caaagtttcg cgagcttcgt gcttgcatcg tcaaatcctc aacatatcag
     2521 gagagagggt aggattaagc ggttctaagg ttttgccact tcctgttggt gggcagtttg
     2581 agggtctctt tacaagcgtg atcgatgaaa tgcttccaac tgacgattgg cctcggtgat
     2641 gactgccaac ttactcacga cgtcgggcac cctggcaccg ggaatggcgt aatagccctc
     2701 gtcatccccg agtccggtat agacgcggtt tccaatacag ccaaaactgg ttgccgcatg
     2761 tcccgattgc agtgattccg gcagcacggc gcaggtcggc cttcccatgg tcgcacttcc
     2821 gccgggtaca cccgctgcct gtgtcgcttc tgccagaagc atcagctgct tgacggtccc
     2881 tcgaaccagc accacgtccg gagtgaacgt ggcctgggtg agtggcgcat aaacggccac
     2941 gtgaaaggtc tcctctcgat gcgggatcat cggaatttct ttcatcgtca ggtactgcat
     3001 gtctaccatc gtcttgacca gaccattcag ctccgttgcg acctggggtg gaagctccac
     3061 accgtgggta tgggctccga ccggacaacc aaagtgatcg gatccttccg tatagaaggt
     3121 ccggccctca gccgcgagct tccaatagcc gcatcctgcg ggtgcaggag tggtgacccg
     3181 tggaatgccc gcaggtgccg tgtcccgaaa tgcgatggcc actgccggga ccttcaatcc
     3241 taaaagttcc tgaatcttcg tgccttgtgt tttgagttca gcttccacgt ctcatcccct
     3301 tctcaactct atatgtgact ctacccagcc attgttgcag gatgctgaaa aatccgccag
     3361 cgtcgatccc tgcctccgct ggaacagctt cgcgcaggca ggtcgcttcg ttcagaccct
     3421 caacgtagag ggtctgcctc gggccttcat ttgctgcggc tttcctttcg agggcctcag
     3481 gacaggactt gacggctatt ttgattattc tgcgggagca tttttctatt gtgccacatt
     3541 cgcggactac cgaggtttca gcgagcccac atcgttttct cgcaggctgc tagatttccc
     3601 catgcccctt tgtcagtaac ggtttgaatt gctgggtcgt cggctcagcg cagcagaacc
     3661 atgattccgt taaccggggc ggcttcggac gatcaggcct gctcacccgt gtccgagttg
     3721 aggtctcaac gggccacccg gctatcttat cagcattcgc tcgaatacga tcaaaggtga
     3781 ccatcgcatc ttcctcttcc ttggcactcg cttccacgat tgcacttacc gcctgatgta
     3841 gttgatccat ccggtcatcc ggatgcgtcc atggatactg aaagctggcc tgatccagtg
     3901 gaccgagaaa cctctgaatg tcagacttgg cgagaagagc cgagccggga ggcaccaaga
     3961 gacgaatgct caactgcacc gggtcggtcg cgtcgataag atcgtggtct tccaccatgt
     4021 ccaacatatg gaggtaatcg tccaggctgg tccagggcgt gaaagccacg agcgacgggc
     4081 gaagcgtgat gccggcttcg cgcagaatgg cgagcgctgt tatgatatca gctcctgtat
     4141 gccccttatg caattgttcc aaaacatggt cactgaacga ctccacagcg gagatgatga
     4201 acaggcaccc ctgtgcttca aaatccggca tcagagcacg gtgcttcaag aggtgttcga
     4261 tcttcgcggt gaaatcaaag gtcagttgcg gaaactcctc atgcatccta cgaacaatgc
     4321 tgagggaatg ccccggtccg tttaaaaaat caggatcgcc gaaagtgata tgcgtggcgc
     4381 ctgcatgtac ttgatgccga atgtcctcga ggacgacggc ctccgggaca aggaagaatc
     4441 gcccttcata gaccggcacg atggggcaat gaagacagag atggaggcat ccacgactgg
     4501 tctcgacgta tccgactaat cgttctgtcc cgttctcttc gagccgggca tattggtcca
     4561 acatcgggag tcctgtccga cttgggattg gttgggatgt cgttgaagtg gttgagtagg
     4621 gtcgcttcaa atagggagca acgacccgat tcagagagct cactccctcg agatggttga
     4681 tcgtctgatc tcgatcgagt gcctcaatga gtgatcggag cgggatctca tattctccgc
     4741 ccaccacagt atcggccaac tcctggagta aatattccgc attaatcgag gcgtacaggc
     4801 catagaaaca gatgtggcag tctggatttt tttcgcgaat cagctcggct acgcgcacgc
     4861 caagcctgag ggcggtgtgc atgggtacgg aaataccgac aaatttggcg agcctgagag
     4921 tcggttcatc aatggtttca acggcgatgt ccaggccaac cggcgaaaat ccgacttgct
     4981 ccagaaatcc catcggctgg gcgatgccaa gaggttggtg acccaattca taacatgaga
     5041 caaacatgat cgcgcccggg ggcctgagca acaatcgttc ttttgtaggt tcggtaacgg
     5101 gttccatgtt ccattgctgc cttccgtggt gagatgtcac tggtagcaca cgcctgggag
     5161 ttggcgcaaa tgaggtctct gtgggcactg gggataaaat ctaatggcca aaaccgtgcc
     5221 ttggctcggt catgtcctga ggccaaactt gttgagtgtt gtgatcgaac aaaaaggtgt
     5281 ggagactttc tctgccagtc cagagcaaca aaggggctgc gtcctttatt gggagccgcg
     5341 gttttcgttt ttggaatgga ggtggactcg gcataaagga ttctctaact gccaacctta
     5401 agggtttggt gtcgtatccc tcagatacca acaacgcaag tagtgggtga tcgtttgttc
     5461 tggagattct cactatcgga gttgctagct ataaacagga tggagttatg aattggtttt
     5521 ataactttct acttggttcc taccggctat aaatcaatta ttcagcaaat tcttgttctg
     5581 ggttgatgtt tgcaggagtt tttttagtcc atcggatagt tccaatcttc agattgtatg
     5641 attttcccta acgttcgtga gtatttcata gttccaacct tcatggagga cagtgggtag
     5701 ggataacgtc tctcatttat cttatgaaat ctaaggggtt tttccgctaa gtctcatccc
     5761 aattaattgg tctgcatttt gcttttaatt aaattaggtt tgtcgttttt tcatatagtt
     5821 ctttttagtt agtccgatca tttgtatcgg ttcttcccac ccccttcacc catggtttcc
     5881 tggttgaaac cggtttgaaa gagaggccat cttatgaacc acctgccttc gtccccccta
     5941 ttggcaaaag acttaatgga ttccgaagcc ttaccactat gccgtgggaa attcgaaaac
     6001 gatttggcgc tgcaattcct ctccggtcaa tattgcggat ggccggtcgt tgattccgaa
     6061 caaaaaattc ttgggatcgt gtcagatgcc cgtctgttcg aggcggtttc acggttgtat
     6121 tcacttgatg atcttaaagt cgaagacacg atgaccccac ctatatatgt gcatgaggat
     6181 gaatctcttg ataaggtcct ggatgtgatg acgcaccaac acgttcagag aatgcctgtc
     6241 gtgcgcaacc ataatcttgt cggagtcatc tcccgggcaa gggtgttgca gcattgtctt
     6301 tcgtcaccct cggcttcttc ccgatcagtt cctaggtgtg cgtggtgtga acgggagcag
     6361 gattcctctg ccgcgatggt tagcaccaaa gagggacgga atttggcttg gtttttatct
     6421 atagaccaac tcaaattttc tgagatcgat ctcgcgcata cctattgccc tacttgtgtc
     6481 aaaactcttc gggcagtcaa caccacatcc gggaatctgt cctccgataa aagggagatc
     6541 cgggaaatcc ggccatgctt gttggtggtt gatgatgatc caacagtgac cggactatta
     6601 acgcatgcgc ttcaggaatg gggatatgag gtacttgttg cgagaaatgg atgggagggg
     6661 ctggccgttg tgagccgcca aacggtggat ggaattttat tagatatgga tatgcccatt
     6721 atggatggga gaaccatgct ggatgaatta cgatggttgg gccatcaggt gccagtgtta
     6781 atgatgtctg gggcaacgga cgaacgagct ctccggcaat tgttacatga aggcgctcaa
     6841 gggttttttg tgaaaccgtt tcatctgaaa tctctcaagg aagcctgtcg gaagatcctt
     6901 caaaagaatg tgtcccaggc atcctctcct tcccatgtgg cttgatatag gcatgtataa
     6961 cttattgatg gaggtaagaa tggaatccat ctgtgagttt ggaaaatatt tgcgcaggag
     7021 tgagagagcc ttcataaggc aatgccttcg cgacccaaca agcttcttaa gctctgaatc
     7081 acagggaaaa cctcctggtt ccagtcagcg ccctggcgat caaaggcttt ctgttcttcc
     7141 tcaacaatcc aacgatcttg tgcaaaaatt ccatcggtaa accacacaat aaacggccac
     7201 aagaggtgga tgaggcctgg aattgaaggt ttcctgatca tcatcaaccc ataggtgtga
     7261 ttgattcgtt gcgcaggatc aatgggaaca tacacattcc agagatttaa tgccgggtaa
     7321 gtactcccgg ctgtccaaaa cttgagcgtt tggtaggggt agtttgtaca gatggtcatc
     7381 agatcatgtt ttgggccatc tgctgatggc ttggtactcc ccagaatgag tttttctccg
     7441 agtggttgtt tgccttttat tcgagagaag gtgtagtcga cttccaccgc atgcggcccc
     7501 tctcgtagtt ccaggaagac cgttcgaatc ccgcccatca gtcgacggtg caaaaactga
     7561 tggttcatat ccataaggtt ctcatgcatg aatgagtaat ggcagcccac ctgacggtca
     7621 aggtatcgcg ttttatattg cgaatccgct gctaaggaca ccttcgggaa ttcaacttcc
     7681 tccactctgc ccaccgagcc cggaaataca aacaccaggc cataggcttc ccgacaggga
     7741 taacttctga ctcctgacgg tcgtgcttgg tttttttcta aatatggtac acccacgcat
     7801 cgccccgtct gatcaaaagt ccaggaatga tatccacact ggacatgttc tccatgcacc
     7861 actccgcaat ggagcggtac ttgtcgatgg gcgcatcggt cttcgagtgc atagaccatt
     7921 ccggttacgg ttctcaccag cacaatcgga taaccggcaa aggacactcc aagcattttt
     7981 ccctttttca gatctctgga gcgggcgagg ggataccaat tgtccgggtg aagaccggtt
     8041 ttccttaaat cggatgtgag aggcgattgc ccagaccggg acgcagaaca ttccaggata
     8101 atgggagcgc cgttttcctt ctgtctgtct atgggtgttt gcgattgttc aatcatgaaa
     8161 ttctccaaaa atgaagacaa ccgtgatagg aaacgcctgg gggtctttag cgatatagag
     8221 aaataactgg aagtgtatga ggtttgggtc aggcattaag cgagctgagg aaaaagggga
     8281 tagggactcg aataattcct tactggggca gagtataaat cgtgtgatca aaaatatcga
     8341 gtcggagcga atctttttcg aaagcctccc gggtcaccgg caattgccat tcccacccgc
     8401 catgtgtttg gggaaagatt gaatcgttta agatatgatg agaaaatcag gaaattcagt
     8461 tgaaattcat ttatggtatg gattgaacag ccggagtcgc catcaaagga tgaagtgtcg
     8521 tgaaaccacc agaacgttca tggttaggaa caggatcaat cggacgagcg ttaatggttc
     8581 tcaccacggt ctttatttta gccttgttcg gcatcctgct ctatacgatc accacaattc
     8641 agaatcaaag actcgacagt gtgacggtgg atctggctgg acgacagcgg atgttgtctc
     8701 aacgactcat gaatgaaatt ttattgacgt cccaaggaat tcctgctgat taccggttca
     8761 cccaacagat gttgacccaa acgctggatg cgttacttgt tggtggacaa gccgtaataa
     8821 acccgaaaaa tggcgaaaag gtggccctca cttccccccc atcccaggaa atattacagg
     8881 cattagggga gcaacaaaca ctcttggcgg aattcatgca tcgggccgat acctttttga
     8941 aaacgcaatc cgatcatccc cgatattctt ttgaattaga cgggcttctg gctgtcaatg
     9001 tccggttgat tgaggcagcc aacaaggccg taaagttata cagcagaaat tcccaagaaa
     9061 agatctcgac catgatcgtt tgggaatctc ttatcggtat cttggtgatc ccatttggaa
     9121 ttctcatgac gagacaggtc aaactggcta atcaggagct tgaacatgaa attcaggaac
     9181 gctccaggat agaaacggct ctgcgttatc gaatcgaaat tgaaaacctc gtgacgaatc
     9241 tttcaactca atttatcagt cttgaaacac aagatttgga tgcggggatt aatcgagcct
     9301 tggaagccat tggcactttc ggaggtgtgg atcgaagtta tgtgtttgtc attgtggacg
     9361 acggaaagac gatgaacaat acccatgaat ggtgccagac gggtatcgag cctcaaatca
     9421 caagacttca ggggcttcaa atgcatgatt taccctggtt tgctgaccgt ctgacctccg
     9481 gcccgttttt tcaagtatca agtgtggcaa atcttccgct tgaggcgagt gccgaaaagg
     9541 aggtgtttga aaggcagcaa attcaatccc tggtgattgt gcccatggtt ttacggggga
     9601 aattgttcgg gtttcttggg cttgattcag tgaggcatga aaaaacctgg tcggaagagg
     9661 acatcaggct cctccaaatg gcaggagaaa tatttgtgaa tgggtttgaa cgacaagagg
     9721 tagaagaaaa tttaaggaaa agtgaagccg ccaaagttga agcctttcgt caaagcgatg
     9781 ccctgaaaac agctctgctg tccttggttt cgcacgagct acgaactccc cttactgcca
     9841 ttaaggcttc cattgctgga ttgattgagc tttccaggca ggacgcgtcc aagatgcagc
//
LOCUS       DAORAQ010000093         9888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1097_length_9888_cov_68.311502, whole genome shotgun sequence.
ACCESSION   DAORAQ010000093 DAORAQ010000000
VERSION     DAORAQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1097_length_9888_cov_68.311502"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="PKM72_09110"
     CDS             complement(<1..1973)
                     /locus_tag="PKM72_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNP60985.1"
                     /translation="MSEVHILRDVLIIFSISVLVVFVFQKIRLPAVAGFLVSGTLVGP
                     HGLNLISDLHQVETLAEIGVVLLLFTIGLETSLSRIRASRRLLWVGGPLQILSMLSGV
                     VLGGWLLGRSWEESVFWGLLLSLSSTAIVLKILGDRGEMDALHGQATMAILIFQDLAV
                     VAMILLTPVLGTGSQGETGVIVETLVKSVMVVGLIVLAARVLVPHVLHLIVGTRSREL
                     FLLSIIVLGLGTAWLTSLAGLSLALGAFIAGLVISESEYSHQALAEVIPFRDSFNSLF
                     FVSVGMLMNPSVIFEFPLLVMGMLVLVVLGKFLTGTGAVFLAGAPVSSALLTGVALAQ
                     VGEFAFILARVGQGEGLLDTQAYNIFLAVSVLSMTITPFLIQWAPRLARRTEALDRLK
                     HWFPRRQSHASQSTKIKVKDHVIIVGYGLNGRNLARVLGDMEIPFVVLDVNPDVVQAD
                     QKGRGTILYGDGTNPRVLTQAGIILARVLVVATSDPFGARRIVQQARQLNPTLHIVVR
                     TRYLKELQDLQELGANDVVPEEFETSIEIFALVLRTYQTPKAMIQDKVEQIRREGYLL
                     LRRGELPELAHHLRAGTLADVQVDTCRVENDSHVLGKPLRQLHIHGRTGASVIAITRN
                     GVTQSNPSRETILEPGDVLVLLGAREQIRKAIAL"
     gene            complement(2225..2860)
                     /gene="leuD"
                     /locus_tag="PKM72_09115"
     CDS             complement(2225..2860)
                     /gene="leuD"
                     /locus_tag="PKM72_09115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNP60986.1"
                     /translation="MQAFTILTGTVAPLNRGNVDTDQIIPKQYLKTIHRTGLKEGLFA
                     DWRRRADGSQDPEFFVNQSCYQQATILLTRENFGCGSSREHAPWALLDYGIRCIVAPS
                     FADIFYNNCFQNGILPVELPGEVIQTLFDRVAHTDQYQITVDLAQQIVILPEGEKYSF
                     TLDPFRKDCLLKGLDGIGLTLQHESAITAYEKRRAQEAPWLFQDFSSSVHS"
     gene            complement(2930..4336)
                     /gene="leuC"
                     /locus_tag="PKM72_09120"
     CDS             complement(2930..4336)
                     /gene="leuC"
                     /locus_tag="PKM72_09120"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNP60987.1"
                     /translation="MASKTLFEKIWDQHVVREEPDGTTLLYIDRHLVHEVTSPQAFEG
                     LRLAGRRPRRPTAALAVPDHNVPTTDRSQGIADPMSALQISTLESNCAEFGITYYGME
                     DIRQGIVHVIGPEQGLTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEIRVEGTLPDYCSSKDVILAIIGQIGIGGGTGYVVEYTGSVIRSFSMEGRM
                     TLCNMSIEAGARAGLVSPDELTVAYVKNRPLSPKEEVFQQAKSDWLKLKTDPGARFDK
                     VVELRGEDISPQVTWGTNPGMVTDVNGHVPDPASIPDEKLRMATERALAYMGLPGGIP
                     ICDIRVDRVFIGSCTNSRIEDLRVAARYAKGKHVAQSVKAMVVPGSGLVKHQAEEEGL
                     DRIFRDAGFEWREPGCSMCLAMNADVLTPGERCASTSNRNFEGRQGKGGRTHLVSPAM
                     AAAAAVEGHFVDIRDWKM"
     gene            4770..5813
                     /locus_tag="PKM72_09125"
     CDS             4770..5813
                     /locus_tag="PKM72_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNP60988.1"
                     /translation="MGIFNYFISHFSQDMAIDLGTSSTLIYIKGKGIVLNEPSVVTVE
                     TNSKKLLSVGEEAKRMIGRTPGNLTAIRPMREGVIADFDMTERMLRHFIQKVHRGGTL
                     LRPRIIIGVPSRITQVEQRAVKESAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDIGGGTTDIAVISLGGIVYSESVRVAGDQLDGAITNYLKREYSLLIGEHMAERIKME
                     IGSAYPLPEKKQMAVKGRDVVSGIPRTILVDDNEIREALHDCLTTIIRAIRLALENTP
                     PELAGDIIDRGIVLTGGGSMLQGLDNRLREETSLPIVTVDDPLTSVVLGVGKTLEEFS
                     LLRKISSHSRLNP"
     gene            5956..6711
                     /gene="mreC"
                     /locus_tag="PKM72_09130"
     CDS             5956..6711
                     /gene="mreC"
                     /locus_tag="PKM72_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNP60989.1"
                     /translation="MGGPFARILEVPLHAVASVQNSIRQAWDHYIALQDAWEENQLLR
                     QEVQRLQGEKNFLREKAIIATQFQQLLAYQETTPMTTLPARIIGRNVSNWYRSMIINK
                     GSQDGIHQEMGVITDAGVVGRVVRVNPTTSIVLLLSDPNVAITGMIQKSRDEGLVQGT
                     PQGTIHMKYLPPLSPVQPGDPVVTSGLTNDFPRGLQIGRIHEVTKADTDLFQLGEIEP
                     IVDFTKLEAVLVITSFQPALPPSLPKPAVSPSP"
     gene            6721..7230
                     /gene="mreD"
                     /locus_tag="PKM72_09135"
     CDS             6721..7230
                     /gene="mreD"
                     /locus_tag="PKM72_09135"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNP60990.1"
                     /translation="MTIFLNILLCFGVVLAQAVFSTILSVKGIYPDLCFILACLIGFL
                     SGEYKGLMIGLTVGLFQDLLTPGGIGLNMVIKALAGGLAGITTHTFSTVTSASVLLIT
                     FALSLGCGLASLVVAYPELNSLDLLHAFSSGLIPQGLYNSLLTLVMFWLIKFIGQSVN
                     MVHLGQKPR"
     gene            7241..9094
                     /gene="mrdA"
                     /locus_tag="PKM72_09140"
     CDS             7241..9094
                     /gene="mrdA"
                     /locus_tag="PKM72_09140"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNP60991.1"
                     /translation="MLDTSRQANELADIQNRLIFVKIGFLMLLVLLGLRLWQLQVRDG
                     LHYQDLARDNRTRTIVLEPARGLLYDRNGELLANNIPSFQLYLSLEDLQDREALLAQL
                     PQFVDIDQQELSHKLALKSRRGRVKIKADITLKEASLIESHRLELPGVVIQPEYQRHY
                     PLENYASHVLGYVGEISESQLKEPGFSDLQGGRIIGQNGVERTFDEYLLGETGEEVIE
                     VDALGYPKRSLSVRRPLAGDDLYLTLDIRLQRLAEDLLGEEAGAIVALDPWNGDVLAL
                     ASKPGFNPNDVSGGISAKDWQRFLKDTRHPLTNRAIQGQYPPGSVFKIIMAAALLETQ
                     TLSAQEMIPCHGTFPFGRRVFRDWKAGGHGQVDLTKAIAESCDVYFYKAGNQLGVDPI
                     STFARQFGLGEKTGIALPSERSGLVPSTEWKKRVKQEPWYPGETISISIGQGFLTVTP
                     LQMAKVASIIATNGRVVQPRLLKASRLRRTGNLKEVPEPPIRQLAISPQTFAHIKEGL
                     AAVVTRGTAKLAQSEFVNIAGKTGTAQVVALKPDGDKDKNKETQKEFRDHAWFVAFAP
                     VEHPKIAVAVLVEHMGHGGSASAPLAKTLIEAYMGYETTQQPAPSLRNPIP"
     gene            9116..>9888
                     /locus_tag="PKM72_09145"
     CDS             9116..>9888
                     /locus_tag="PKM72_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNP60992.1"
                     /translation="MSLMNDNVNRRGLDTFDWCFLGVIMAIISLGIVSIYSVTNTQAT
                     TGIPIYLKQATWMVVGSLAFLVAVGIDYHKLARFSYLLYGIGLILLIFVLIAGKTSRG
                     SQRWIPLGPFAVQPSEFIKIPLLLMLATYYGTTTREGWVRRLIIPGIIVLPGFLLILK
                     QPDLGSSLGFLAIFLVVVLTVGMKSKAFGFIVLAALMLFPFAWGGVWGSLHEYQQDRI
                     LSFVDPNSDPGGKGYHGLQSRIAIGSGQILGKGLYGGTQT"
BASE COUNT         2596 a         2668 c         2266 g         2358 t
ORIGIN      
        1 agggcaatgg ccttgcggat ttgctcacgc gcaccaagaa ggacgaggac atcacctggc
       61 tccagaatcg tttcgcgaga aggatttgat tgtgttacgc cattccgggt aatcgcaatc
      121 acagaagcgc ccgtccgtcc atggatatgc aactgtctca atggtttccc caacacgtga
      181 gaatcgtttt ccacccgaca ggtatcgact tgcacatcag ccaatgttcc agcacgcaga
      241 tggtgagcta actcaggtag ctcccctcgc cgaagcagga gataaccctc cctacgaatt
      301 tgttcaactt tgtcttggat catggccttc ggcgtctggt aggtacgaag aaccaaggca
      361 aaaatctcaa tggatgtttc gaattcctcc ggcacgacat cattggctcc taactcttgg
      421 agatcctgca attcctttaa atatcgtgtt cgtacgacaa tatgaagagt gggattcagc
      481 tgtcgtgctt gctggacaat ccggcgcgct ccaaatgggt cagaggtcgc gaccacgagc
      541 actcttgcca atatgatccc ggcctgagtc aacaccctgg gattcgtccc atccccatag
      601 agaatagtgc ctcggccttt ttgatcagcc tgcacgacat caggattaac gtccagcaca
      661 acaaatggaa tttccatatc acctaagacc cgtgcaagat tccgcccatt taaaccatag
      721 cccacgatga tgacatgatc tttgactttg attttggtgc tttgtgacgc atgggactga
      781 cgtcgaggaa accaatgctt caggcggtca agcgcctcgg tccgtctagc cagacgggga
      841 gcccattgaa tcaaaaatgg cgtaatcgtc atagacaaca ccgatactgc aagaaaaata
      901 ttataggctt gcgtgtccaa taacccttcc ccctgaccca ctcgtgcaag aataaaggca
      961 aactctccca cctgcgctaa ggccacccct gttaataatg cagaagatac cggagcccca
     1021 gctaggaaca ccgctccggt tcccgtaagg aattttccca aaactacaag caccaacatc
     1081 cccatcacca ggaggggaaa ttcaaagatg accgagggat tcattagcat ccccactgag
     1141 acaaaaaata aactattaaa actatcccga aagggaatga cttcggcgag cgcctgatga
     1201 ctatattcag attcagaaat cacgaggccc gcgataaagg caccaagagc caatgacagc
     1261 cccgcgagag aggtcaacca agcggttccc agccccaaca cgataatcga caagagaaat
     1321 agctctcggc ttcgagtccc cacaataaga tgaagaacat gagggaccaa aacccgtgca
     1381 gccagcacaa ttaaccccac aaccatgacg gatttcacaa gcgtttcgac aatgacccct
     1441 gtctcccctt gagaacctgt ccccagtacc ggagtcagta aaatcatagc caccacggct
     1501 agatcctgga aaataagaat cgccatagtt gcttgcccat gtagcgcatc catttcccct
     1561 cgatctccca gaatcttgag gacaatggcg gtgctgctca acgacaacaa cagaccccag
     1621 aagaccgatt cctcccagga tcgacccaat aaccatcccc caagcactac gccactgagc
     1681 atgctcaaaa tctgcagggg accaccaacc cataacagcc ttctggaagc ccgtatgcga
     1741 gaaagagagg tttccaagcc aatggtaaac aacaacaaca ccacaccgat ttcggcaaga
     1801 gtttcgactt ggtgcagatc agaaatgagg ttcaacccat ggggacccac taaggtgcca
     1861 gaaactaaaa atcccgcaac tgccggtagc cggatttttt gaaaaacaaa tacaaccaga
     1921 acggagatag aaaaaataat gaggacatct cgtaggatgt ggacttcact cataagtcga
     1981 gtgggcggcg atggcaggga ttcagcgtat tccccatacc tgacatagac acgataaaga
     2041 agtttgggtg gatttcaaac acccatcatc cctcggatcg agggaaatgt ctactattcg
     2101 ctttgtccca ctcatctgag atagaaccat gctattacgg ataggggagg aagaaggatc
     2161 aaggggagaa tcgagatggg gcattccaga cccatcccga tcaattgaga aaattaatag
     2221 ccttttacga gtggactgaa gaggaaaaat cctgaaagag ccagggagcc tcctgagccc
     2281 ggcgcttttc ataggcagta atcgcggatt catgttgaag cgtgagtcca attccgtcca
     2341 atcccttcaa caaacaatcc ttgcgaaaag gatccagggt aaaggaatac ttctcccctt
     2401 cgggaagaat gacaatctgc tgagctaagt cgactgtgat ctgatattgg tcagtatgag
     2461 ctactcggtc aaacaatgtt tggatgactt ctcctggaag ctcgacgggg agaatcccat
     2521 tctgaaaaca attgttgtag aaaatatcgg caaaactcgg agccacaata caacgaatgc
     2581 cataatccaa caaagcccat ggagcgtgct cccttgagga tccacatccg aaattttcac
     2641 gggtcaatag aatcgtggcc tgttgatagc acgattggtt gacaaaaaac tcaggatcct
     2701 gagacccatc cgcccgccgc cgccaatcgg caaataaccc ctctttgagg ccggtgcgat
     2761 ggatcgtttt caaatattgt ttcggaatga tttgatccgt atcaacattg ccacgattta
     2821 acggagcaac cgttccggtc aatatggtaa acgcttgcat atctatctcc cgtttaaatg
     2881 tagtggaagc agatgatctc ttgaaggcaa aagaattccc ttattgatct cacatcttcc
     2941 aatcacgaat atcaacaaaa tgtccctcaa cagcagcagc agcagccata gcaggggata
     3001 ctaagtgggt ccgtccaccc tttccctgac gcccctcgaa gtttcgatta ctggttgagg
     3061 cacatcgttc tcccggtgtg agcacgtccg cattcatggc gaggcacata ctgcatcctg
     3121 gttcacgcca ttcaaaaccc gcgtcgcgaa aaatccgatc caaaccttcc tcctccgctt
     3181 gatgtttgac taaccctgat ccaggcacca ccatggcttt gacgctttgg gcaacgtgct
     3241 ttcctttggc ataccgtgcc gccacccgta aatcctcaat tcgtgagttg gtgcaggacc
     3301 cgatgaaaac tcgatcgacc cggatgtcac atatagggat accacctggc aaccccatat
     3361 acgctaaggc acgttctgtt gccatccgca gtttttcatc cggaatggat gcaggatcag
     3421 gcacatggcc attcacatcg gtcaccattc cgggattggt cccccaagtc acttgaggtg
     3481 aaatatcctc ccctcgcagc tcaacaacct tatcaaaccg ggctccaggg tctgttttca
     3541 atttcaacca atccgacttc gcctgctgaa atacctcctc cttcggacta agcggccgat
     3601 tcttcacata tgcgaccgtc aattcgtccg gactgaccag accagcacgc gctcccgcct
     3661 caatagacat attacaaagg gtcatccggc cttccattga aaacgatcgg atcaccgaac
     3721 ccgtatattc cacgacatat ccggttccac caccaatacc aatttgcccg ataatcgcca
     3781 gtatgacatc tttggaagaa caataatcag gaagcgtgcc ctccacccga atttccatag
     3841 tcttgggtcg tttctggacc aaacattgcg tcgccaacac atgctcgact tctgaggtgc
     3901 caatgccaaa cgccaaggct ccaaacgccc catgagtcga tgtatgggaa tctccacaca
     3961 caatcgtcat ccccggtagc gtcaaccctt gttcaggtcc gatgacatgg acaatacctt
     4021 gacggatatc ctccatccca taataggtaa ttccaaactc tgcacagttg ctttccaagg
     4081 ttgaaatttg caaggcactc ataggatcgg caatgccctg cgaccgatcg gtggttggaa
     4141 cattgtgatc gggaacagcg agcgccgcag ttggacgcct tggcctcctg ccagccaacc
     4201 ggagtccctc aaaggcttgc ggggaagtca cttcatgcac gaggtgtcgg tcaatataca
     4261 ataaggttgt cccatctggc tcttcacgaa ccacatgctg atcccaaatc ttttcaaaca
     4321 atgttttgct tgccatggaa tgcatccttc tttctttttg aaaatttcgg actctaatta
     4381 tctacatttg ctcccttctg gcaagtgcca ggaggagctt tttccagatt tggtttcaga
     4441 tccgcctttt ctttgtggtc ttttaagact tctgatatac ttttccctac gacagtgaac
     4501 aatccaatct gaaactttca ccttctcgat acttaggtgg aaataagtgg ataccggcta
     4561 tcattcatcc agccccgaac caatctaaag ggtttccggc cagcggcgaa gaacgactca
     4621 acgtcgaata tcaaggttaa ccggaaaatc agacatagaa aagtaaagtt gatctatttc
     4681 ccctgtagca cgtaaagact ctaacaatcc tatttccgac tccgccattg tcgcccaccc
     4741 tctgaaattg ataggtctca acacttctca tgggtatatt caattatttc attagccatt
     4801 tttcccaaga catggccatc gatttaggaa cttcctccac attaatttat atcaagggca
     4861 aagggattgt cttgaacgag ccctccgtgg tgacagttga gacaaactca aaaaaacttc
     4921 tgtcggttgg ggaagaagca aagcgaatga tcgggcgaac ccctggcaat ctcactgcca
     4981 ttcgtcccat gcgagaaggt gttattgctg attttgacat gacagaacgc atgttgcgcc
     5041 attttatcca aaaagttcat cgcgggggaa ctctgctcag gccacgaatt attattggcg
     5101 tcccatccag aatcacgcaa gtcgaacaac gtgccgttaa ggaatcagcc gaattggctg
     5161 gtgcacggga agtttatctc atcgaagaac cggtggccgc tgcaatcgga gcgggattgc
     5221 cgattaccga gccttccggc aacatggtgg tggatatcgg aggaggcaca accgatattg
     5281 ccgtgatctc cctgggaggg atcgtttaca gtgaatcagt ccgggttgcg ggcgatcaat
     5341 tggatggagc catcacaaac tatctcaagc gggaatacag cttgctaatc ggcgaacata
     5401 tggcagaacg catcaagatg gaaatcggct cagcctaccc tctgcctgaa aaaaagcaaa
     5461 tggccgtcaa ggggcgagat gtggtatccg gcatccccag aacgattctt gtcgacgaca
     5521 atgagatacg agaagcttta catgattgcc tcaccacgat catccgagcc attcgtctgg
     5581 ccttggagaa cactccgccg gaattggcag gagatattat tgaccgaggg atcgtgctga
     5641 ccggaggcgg atcaatgctt caagggctgg ataatcgact acgcgaagaa acgtccctac
     5701 ccattgttac tgtcgatgac cccttaacct cggtcgtctt aggagtagga aaaactctcg
     5761 aggaatttag cctccttcgg aaaatttctt cgcattccag gctgaatccc tgagcttccc
     5821 tctatttgca tgcctcgatc agagccgaat tttcccatac ctacaagtat ccgtcgagtt
     5881 ctaggagtta tcttcattct attcctcgca ctcctgctgt ttctgccgcg acaatctcag
     5941 gaatggttaa gtcatgtggg aggacctttc gcacgaatat tggaagtccc cctgcatgct
     6001 gtagcctcgg tacagaattc tatccgtcaa gcgtgggacc actacatcgc actacaggat
     6061 gcctgggaag aaaatcaact gctaaggcag gaagttcagc gcctacaagg agagaagaat
     6121 ttccttcgag aaaaggccat tattgccacg caatttcaac aattactggc gtatcaggaa
     6181 accacgccca tgacgaccct tccggctcga atcatcggcc gaaatgtgtc caattggtat
     6241 cggtccatga tcatcaataa aggcagtcaa gacggcattc accaggaaat gggtgtcatc
     6301 actgatgccg gagtggtagg acgtgtcgta cgagtcaacc ccacgacatc catcgtgctt
     6361 cttctttccg accccaatgt cgcgatcacc gggatgattc aaaaaagccg ggatgaggga
     6421 ctggtacagg gaacccctca aggaaccatt catatgaaat atctgcctcc tctttccccc
     6481 gttcaaccag gagatccagt ggtcacatct ggattaacaa atgattttcc ccgtggactc
     6541 caaattggcc ggattcacga ggtcaccaaa gccgataccg atttatttca attgggggaa
     6601 attgagccaa ttgtagattt caccaaactg gaagcggtcc tggtgattac ctcatttcag
     6661 cctgcgctcc ctccatcatt accgaaaccg gcggtctctc cttctccctg atgtaatcac
     6721 gtgacgattt ttctcaacat tcttctgtgc tttggagtgg ttctggccca agccgtcttt
     6781 agcaccatcc tttccgtcaa agggatttac ccggatttat gtttcatctt ggcctgctta
     6841 atcgggtttc tttcaggcga atacaaagga ctgatgatcg gactgacggt gggactattt
     6901 caagacttac tcactccagg cggaattggg ctcaatatgg tcataaaggc acttgccggt
     6961 ggcttggccg gcatcacaac ccacacattt tccacagtca ccagtgcctc agttctgctc
     7021 atcaccttcg cgttgtcgtt agggtgtgga ttagcctctc tcgtcgtggc atacccggaa
     7081 ctaaatagcc tcgacctctt acatgccttc tcctcaggac tgatcccaca aggcctctac
     7141 aacagcctac taaccctggt gatgttctgg ttaatcaagt tcattggcca atcggtgaat
     7201 atggtacact taggccagaa acctcgataa gacccacaca atgttggaca cttcccgcca
     7261 agccaatgaa cttgccgata ttcaaaaccg actaattttc gtcaagatcg gctttctgat
     7321 gctgttggtg ttattggggc tccgtctttg gcaactacag gttcgggacg gattgcacta
     7381 ccaagacctc gcccgcgaca atcgcactcg aactattgtc ttagaaccgg ctcgcgggct
     7441 tctatatgat cggaacggcg aacttcttgc caataatatc cccagcttcc aattgtatct
     7501 ttctttggaa gacctacagg atcgtgaagc ccttctggct cagttacctc agttcgtcga
     7561 cattgaccaa caagagcttt cccataaact agcactcaag agtcgaagag gccgagttaa
     7621 aattaaagcc gatattaccc ttaaagaggc gtcgctcatc gaatcgcacc ggctggaact
     7681 tcccggggta gtgattcagc cggaatatca acgtcactat ccacttgaga attacgcctc
     7741 acatgtcctg gggtatgtgg gtgagatttc cgaatcccag cttaaggagc cgggattttc
     7801 cgatcttcag ggaggacgca tcatcgggca aaatggagtg gagcggacat ttgatgaata
     7861 tcttctagga gaaactggag aagaagtcat tgaagtcgat gccctggggt atccgaaacg
     7921 gtcactttcc gttcgccgtc ccctggctgg ggatgaccta tacctgactc tcgacattcg
     7981 acttcaacgc ctcgcagaag acctattggg agaggaggcc ggagccattg tcgccctgga
     8041 tccctggaat ggagacgtcc tggcgctggc aagcaaacct gggtttaatc ccaatgatgt
     8101 ctcgggaggg attagcgcca aagactggca acgatttctc aaagataccc gccaccccct
     8161 caccaatcgc gccattcaag gccaatatcc accggggtcg gtatttaaaa tcattatggc
     8221 agcagccctc ctggagactc aaacgctcag cgcacaggaa atgatcccct gccatggaac
     8281 atttcccttt ggaaggcggg tctttcgtga ttggaaagcc ggagggcatg gtcaagtcga
     8341 cttaaccaaa gccattgcgg aatcttgtga tgtttacttt tacaaggccg gcaatcaatt
     8401 gggggtcgat cccatctcca cctttgcccg tcagttcgga ttaggtgaaa agacaggcat
     8461 tgcgctccct tcagaacggt ctggcctcgt cccttccacg gaatggaaaa aaagagtcaa
     8521 acaggaacca tggtaccccg gagagaccat ttcaatttcc attgggcaag gatttttaac
     8581 cgtgaccccc cttcagatgg cgaaagtggc ttccatcatc gcgaccaatg gacgagtggt
     8641 ccaacctcgg ttgctcaaag cctcccgact tcgacgaacc ggcaatctca aagaagttcc
     8701 tgagccaccc attcgccaat tagccatctc tccccaaacg tttgcgcata tcaaggaggg
     8761 attggctgca gtcgtcacca gaggaacagc caaactggcc caatcagagt tcgtgaacat
     8821 tgcgggtaaa actggaacag ctcaggtcgt tgcactgaaa cctgatgggg acaaagataa
     8881 gaacaaggag actcaaaagg aatttcgaga ccatgcctgg tttgtggcgt ttgcacccgt
     8941 agaacatcca aagattgcgg tggcggtatt agttgaacat atggggcatg gaggctcggc
     9001 gtccgctccc ttagccaaga ccctcattga agcctatatg ggctatgaga ccactcaaca
     9061 gcctgcaccc tctctcagaa acccaatccc ttagggacgg agaacgactc aacttatgtc
     9121 attgatgaac gacaatgtaa accgccgagg cctggatacc ttcgattggt gtttcctggg
     9181 tgtaattatg gccatcatct ctttgggcat cgtatccatt tatagcgtca ccaataccca
     9241 ggcaacaact ggaatcccca tttatctcaa gcaagccaca tggatggtcg tcggctcctt
     9301 agcctttttg gtcgcggtag gtattgacta tcacaaactc gctcgttttt catatctgct
     9361 ctatgggata gggctaatct tacttatttt cgtgttaatc gcaggcaaaa ccagtcgagg
     9421 ttcccaacga tggattccac ttggcccctt tgcggtgcag ccttcagaat tcattaagat
     9481 tcctctgcta ctcatgctgg ctacctatta tgggacaacc acccgcgaag gctgggtacg
     9541 tcgattgatc atccccggga tcatcgtgct tcctggcttc ttactcatac ttaagcaacc
     9601 agacttagga agcagcctgg gctttctggc catttttctt gtcgtcgttc tcacggtggg
     9661 gatgaaatcc aaagcctttg ggtttatcgt attggcggcc ctcatgcttt tcccgtttgc
     9721 ctggggcggg gtctggggct cccttcacga gtaccaacag gatcggattc tcagctttgt
     9781 cgatcccaat tctgatccgg gaggcaaggg ttatcacggg cttcaatcgc gcatcgctat
     9841 cggttccggc caaattctgg gaaagggctt atatgggggc acccaaac
//
LOCUS       DAORAQ010000094         9866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1103_length_9866_cov_57.230761, whole genome shotgun sequence.
ACCESSION   DAORAQ010000094 DAORAQ010000000
VERSION     DAORAQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_9866_cov_57.230761"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKM72_09150"
     CDS             complement(<1..200)
                     /locus_tag="PKM72_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60993.1"
                     /translation="MAKISPPTSPSIQKPGSSGSAKEAELKAKEKNGQLIWPILIKVM
                     ICVIPAMGLVGMGKLFAGGKIF"
     gene            complement(491..2395)
                     /gene="asnB"
                     /locus_tag="PKM72_09155"
     CDS             complement(491..2395)
                     /gene="asnB"
                     /locus_tag="PKM72_09155"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNP60994.1"
                     /translation="MCGIAGWLGHLTDSESYAARVAKALHHRGPDAHGIQNWPEATLI
                     HTRLSVIDLSPGGAQPMANEDGRIWVVFNGEIYNHRELRQDLEARGHIFKGRSDTEVI
                     VHLYEENGAAFLNRLRGMFALAVYDTKTDTLLLARDRFGIKPLFYAPGTNRLSFASEI
                     RALRELPEINLQPDRQAISDFAALFYIPAPETFFVGIRALQPGEVLFAQMDTHKISWN
                     IQTYHHWAIAPDFEMSLEQAKAQADSLITMAVRRQLESDVPLGALLSGGIDSSLVSVA
                     AQEALKARLQTYNAQFSEEEYDETWAAVAVAKHIGSHHKTLQVGNSRGTWDHITDLLL
                     HAGQPFADTSFFAVNAVSRLMREHVTVALSGDGGDEGFGGYGHFLRIGEIARYQRLPA
                     WLWRTAGAISHPLARLGVLRNSFPDRIRDLTGADDTMILQTLFCWIRDEEHKNLCLDT
                     NLLPVRRLFESQWEYHSSRGVSRLERLSAHVTEVDVRLRMSNDFLFKVDMASMKESLE
                     IRVPLLDEELFAFGLTLPHRLKVSGGMCKKVLRAVAENKLPKAVAEKPKLGFVIPVDK
                     WVDREFKSRLSEVLLGPSSRLPEFFRPETYRPVVEAFCTGRPHHAISRAGLYQRAIML
                     LAVQLALCDR"
     gene            complement(2899..4167)
                     /locus_tag="PKM72_09160"
     CDS             complement(2899..4167)
                     /locus_tag="PKM72_09160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP60995.1"
                     /translation="MPPQKPKVLFLAYYFPPLNAGACVRTWNIAKYLSRLGWDVTVVT
                     PDPHLWRLGDPSQIEKVNIELNENGIKCLLTGHDWRFLSPGCLNYENSGIKWFLGGLG
                     RRIARKWRIEREIGWIKEAKRTCSTLAKEDVDIIFASGSPFCSFGLAKWLADKLSRPY
                     VLDYRDPWTGNPHARYPKPQRIINEEQELLKNCAAVTIVSPSWALDLTRRYGLKEKIH
                     VLSNGFDPDEMSQVQPTQFPHFAIVYTGDFYPPKRVITPFMEALKHLKELRGKENGKW
                     RLHYYGQMGEHVREEAERLGMIDEVELHGKVPRNVVLSAVAGANISLVITSIPDDVTI
                     ADQGMITCKVFESLGLGTPILLICPLGSDVAILDQCKGVRSFQKNNILEMAAYLNERI
                     ESQGERIEGEGPYSWVNLSRQLNDILLKSC"
     gene            complement(4198..5427)
                     /locus_tag="PKM72_09165"
     CDS             complement(4198..5427)
                     /locus_tag="PKM72_09165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP60996.1"
                     /translation="MKIAYIVSQFPSATETFVQSQIVGIIEAGLEVNIFAAKPRDDLP
                     SPESLRKYKLRERTIYQVIPKNKTRRFARGLYLILKYILKHPIPIVRSLNVFRYGRAA
                     SSLTLLYKIIPFLGKGPYDIIHCQFGTDGLQGLYIKQVAAPTAKVVTSFRGFDASKMV
                     FECPDLYKTLFREGDLFLPVSQSLKNLIVQQGCDEGKIVVLPSGINCQNLTGSPKKVL
                     QSERVHVMTIARLIEKKGIVYAIEAVNKIVKSGKSICYSIIGEGELRGDLEELIQERD
                     LKEHVQLLGWKSHEEVIRLLQDAHILIAPSVTAKDGDQEGIPNAIKEAMAMGLPVIAT
                     QHSGIPELVEDGVSGYLVRERDADALADRLSYLLDHPGKWADMGRAGRGRVEKDYDMK
                     TLNNRLVELYGQVCAKT"
     gene            complement(5741..7420)
                     /locus_tag="PKM72_09170"
     CDS             complement(5741..7420)
                     /locus_tag="PKM72_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP60997.1"
                     /translation="MNSKSKVYSTDILFGKATGERIFASNGFERVEYAFDEFCSQLVD
                     VHVKRGVSIHSLRVCSEEGASHIRFSSAATVLNVLHGNEFGMLLTVQYEQKIYTVWLP
                     AACAKLHFDQSQRISEPEDTFLPCHWEVNSGIQLHLFLSGIPGEQAIVPFMIIEDACG
                     EFIGEIKSLNMTERCLYRKTDWFFASKPSDIWRYLINGSIYDPRGHKDLKKRFRCQQC
                     AFAWWSYFGFLYKETGKKLYDIMQNVIAYSVFLDLSPKGEWGHGFWTDEIETHARFHL
                     DGIHLLISQHEKTGESLWVEAAERGLAFVTEHLMEQLDDGSLWFLHDTNEHKDKHAHF
                     HSAIFGKSPGNSLCINTHVQALTVLNRLSHVIPQNKMYSELSEKGLRALQRVLAHQPA
                     EALYRMFIVMWMKSRKKPRSKVEVVAHAVKVRSIRKVFWVLRQHYPRLVYPGGWIDRD
                     LTISCFSEEYQITNLKDFLSLYQQMPLSWLVPYIKNNFNFLRKFLFQFGLTNALESSP
                     YYIEFMDVLYMYDRYIEKIDHEEIERVKEAIRQKTGGYSVDYYASELVREK"
     gene            complement(7480..8451)
                     /locus_tag="PKM72_09175"
     CDS             complement(7480..8451)
                     /locus_tag="PKM72_09175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNP60998.1"
                     /translation="MFNPEKPLVTIAIPTYNRADTYLKHTLQSAKDQSYQNVEIIVSD
                     NCSIDQTEKVVKEFDDPRIRYVKHSQNIGHYNNFKFCIEQARGDYFLLLQDDDLIDED
                     FVDVCLETINYSFMDIGVIRTGTRYIGPDGQQIGEWLNTVGGLSTEEFFLAYLSFETG
                     IYLCSTLLNTKRLREIGGMHSKHNLLLDVMAIAKLSSHYGRKDIRESKASNRKHPNEL
                     TVSVKTKEWCEESLLLVDTMCQLVSEKNMVVKIRKTGNNYIFRHNYNLVKKEKVVSIR
                     LKALLLLFRKCGYLFSISRFLKYAILDEWKAGKRKIKQLIGSFGYEA"
     gene            complement(8758..>9866)
                     /locus_tag="PKM72_09180"
     CDS             complement(8758..>9866)
                     /locus_tag="PKM72_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNP60999.1"
                     /translation="AITGAVKRHLISDVPVGAFLSGGIDSPLVVAKMRSVSAAPIEVF
                     TIGTGEEATDETQDAMAYARDFGVQHRIECMDPAKALDFLEDVIESCGEPFGDYSLFP
                     TMMVSRLASQNYKVMLSGDGGDELFWGYTGRFGSLVRNAGDFRYPFWCRWVWRGFKRV
                     LQYGNYNHLNQRSLGNYHRLMLTHLSEQWLKQIFPSLPSWPNAYKAFEYHSCDPDRAA
                     QYSRISEYECHLPYVLMKVDRASMFHSLEVRVPLLDKEVIEIAAQTDWHQCLNVEQKT
                     GKMILRQILSKYTKQQTQGKRGFEVPMGEWLRTSLREMVEESLMKRDEMLGLEVNKKA
                     LRQLYDQHLNGRSDYSWALWPLLSLSLWINKHYH"
BASE COUNT         2739 a         2443 c         1940 g         2744 t
ORIGIN      
        1 aagaaaattt ttccaccggc aaataatttc cccattccaa ccaaccccat ggcggggatt
       61 acacaaatca ttacttttat gaggataggc caaatcagtt ggccattctt ttctttggcc
      121 ttcagttcag cctctttggc ggaacccgaa cttcccggtt tttggattga gggtgaagta
      181 ggtggactta tcttagccat acctttttcc ttaaacttcg agagcaaccg aaatccacaa
      241 atactctgac ctaaaaaatt gattcatcat gattttccgt agatcactcc cattttcgtg
      301 ctcctctttc gatataaatc ggcttcaaaa tccgctcctt tgtaaggaga ggataggtga
      361 ggtagaggcc gtgggttatc agtcagccac tacggaatca aacattgaag cctcttcaga
      421 ggccgggaaa aggctttcca tgaaggagat tcccacctca tcagcaagac gactgaatac
      481 cgaacccgcc ctacctatcg catagggcta attggacggc tagtaacata atggcccgtt
      541 gatacaaccc tgcccgagaa atagcgtgat gagggcgtcc cgtacaaaaa gcttctacta
      601 ccgggcggta cgtttcaggt ctgaaaaatt cgggaagtcg gctggaaggt ccgagtagaa
      661 cttctgacaa ccgggattta aattccctat caacccattt atccacgggg atgacaaatc
      721 ctagtttggg tttttcagca acggctttag gcaatttatt ttcagccact gcacgcaaca
      781 cctttttaca cattcctccg ctcactttca ggcgatgtgg aagggttagg ccaaacgcaa
      841 acagctcttc atctaacaag gggacacgaa tttccaaact ctctttcatg cttgccatat
      901 ctaccttaaa cagaaaatca ttggacattc gcaaccgaac gtcgacttcg gtgacatggg
      961 cagacaaccg ttccaatcga gaaaccccac gcgatgaatg atattcccac tgtgattcaa
     1021 acaggcgacg gactggaaga agatttgtgt ccaggcaaag atttttatgt tcttcatccc
     1081 gtatccaaca aaacaaggtt tgcaaaatca ttgtgtcgtc agcaccagta agatcccgga
     1141 tcctatccgg gaatgaatta cgcaagacac caagtcgagc taatggatgc gaaatggctc
     1201 cagctgtccg ccacaaccat gcaggcaaac gttgatagcg agcgatttca ccaattctca
     1261 aaaaatggcc atagcccccg aaaccttcgt ccccgccgtc tccagaaagc gcaacggtca
     1321 catgctcacg cattaatcga ctgaccgcat tcacagcaaa aaaggaggta tcggcaaaag
     1381 gttgcccggc atgaagaaga agatccgtaa tatggtccca tgtccctcgg ctatttccca
     1441 cttgtaatgt cttatggtgg cttccaatgt gctttgctac tgccaccgct gcccatgtct
     1501 catcatattc ctcctctgaa aattgcgcgt tataggtttg aagcctggct ttgagagcct
     1561 cctgcgccgc aacactgacc aaggaggaat cgatcccacc gctgagaagg gcaccaagcg
     1621 ggacgtcgct ttccaactgc cgtcggacgg ccattgttat caaagaatca gcttgagcct
     1681 tagcctgctc caaggacatt tcaaaatcgg gagcaatggc ccaatgatga taggtctgaa
     1741 tattccaaga gatcttgtgg gtatccattt gggcaaacaa gacctctcct ggttggagag
     1801 cccgaattcc gacaaaaaat gtctcaggcg caggaatgta gaataaggca gcgaaatcgg
     1861 agatcgcctg cctatccggc tgaaggttga tttcaggcaa ttcacggaga gccctgattt
     1921 cgctggcaaa cgatagccgg ttggttcctg gcgcataaaa tagtggcttg ataccgaatc
     1981 ggtctctggc taatagaagg gtgtccgttt ttgtatcata aaccgctaag gcaaacatgc
     2041 cacgcaatct attcaggaat gctgcgccat tttcctcata aagatgaaca atgacctcag
     2101 tgtctgagcg tcctttgaat atatgtccgc gggcctcaag atcctgccgg agttcccggt
     2161 ggttatagat ctccccatta aaaacaaccc agatcctccc atcttcattc gccatcggtt
     2221 gcgcaccacc aggtgacaga tcgataacac ttaaccgtgt atgaatgaga gttgcttcag
     2281 gccagttttg gataccgtgt gcatcaggac cccggtgatg gagagccttg gctacccgtg
     2341 ctgcgtaaga ctcgctatca gtgagatgac ccaaccaacc cgcaattcca cacataaaac
     2401 tctcactccc tctcgcactg gagactagca atcagctttg gttaaaccca taagccaata
     2461 tcgttgtaaa atttaagacc tgctctgtta gatcttacat cacgccggat tgcataaaat
     2521 attagggaac aaccgaaacg ttttgacctc agggagggaa tgctgggaat cagaccctaa
     2581 ctagcgcatg aacgggagcc catcccgaat ctgactcggc ttaattatca gacggtcttg
     2641 gtttcatttt ggcattgcag cggttattga gatggtaata ttgaaaaaaa tttaacatcg
     2701 ctcctgccca aaatccggaa acgactcccg tgggcttttc cgaccttaga gccctaacac
     2761 gacggcattt aaggcctagt taagatctct ggcattcctg taattccgtt ctgtgtttcc
     2821 caatacccca ggggctttcc cgagataccc ttttccatct acctggagaa tgaaaacaga
     2881 aagggaatgc ccattcaact aacaactttt gagaagaata tcattcaact gcctacttaa
     2941 attcacccat gaatacggac cttccccctc aattctctcc ccttggctct caatacgttc
     3001 attaagatat gcagccattt caaggatatt atttttttgg aaggagcgta cccctttaca
     3061 ctgatctaat atggcgacat cgctgcccaa tgggcaaata agcagaattg gagtccccaa
     3121 acctaaagat tcaaagactt tgcatgtgat cattccctga tcggcaattg tgacgtcgtc
     3181 aggtatagag gtaatcacaa gagatatatt ggcaccagca accgcggata acacgacatt
     3241 gcgcgggact ttcccatgaa gttccacttc atcaatcatc cccaaccgct cagcttcttc
     3301 tctgacatgc tctcccatct gaccataata atgaagacgc cactttccat tttctttccc
     3361 tcttaattcc tttaaatgct tcagagcttc cataaacggc gtaatcaccc gtttaggggg
     3421 atagaaatcc cccgtgtaca ctatagcgaa atgtgggaac tgtgtaggct ggacttgact
     3481 catttcatcc ggatcaaagc cattcgaaag gacatggatc ttttccttaa ggccatatcg
     3541 tcgagttaaa tccaatgccc acgaagggga aacgatagtc acagcagcgc aatttttgag
     3601 cagctcttgc tcttcattga tgattcgctg aggtttcgga taccttgcgt gaggattgcc
     3661 ggtccaagga tcacggtaat caagcacata aggccggctt aatttatctg ccagccactt
     3721 tgctaatcca aaagagcaaa aaggtgaacc actagcaaaa atgatatcta catcctcttt
     3781 agctaatgtt gaacatgtcc gcttggcttc tttgatccag ccaatttccc gttcaatcct
     3841 ccacttgcgt gcgatccgtc gacctaaacc tcccaaaaac catttgattc ccgaattctc
     3901 ataatttaag catccgggtg aaaggaaccg ccagtcatgc cctgtaagga ggcacttgat
     3961 tccgttttca ttgagctcaa tattgacctt ctcaatttga ctggggtccc ccaatcgcca
     4021 caggtgagga tcgggtgtta ccaccgtgac atcccaacct aacctggaga gatatttggc
     4081 aatattccag gttcggacac atgcaccggc attgagcggg ggaaaataat atgcaagaaa
     4141 caagactttg ggtttttggg gaggcattat tctatccttt tcactcattg gcgaatatca
     4201 cgttttggca catacttggc catataattc caccagccga ttatttaaag tcttcatgtc
     4261 atagtccttt tccactcgcc cacgcccggc tcttcccata tccgcccatt ttccaggatg
     4321 gtcaagcagg taggacaatc gatcggccag ggcatccgca tctcgctctc gtaccagata
     4381 acctgacaca ccatcttcca ccagttccgg aatgccgcta tgctgagtgg caataaccgg
     4441 caatcccatc gccatggcct ctttaatcgc attgggaatg ccttcctgat caccgtcttt
     4501 ggccgtcaca cttggagcga tcagtatatg cgcatcttgc aataatcgaa tgacttcctc
     4561 atggcttttc caccctagta actgcacatg ttccttgaga tctcgctcct ggattaattc
     4621 ctccagatcc cctcgtaatt cgccttctcc gattatggaa tagcagatag attttccgga
     4681 tttgactatt ttattgactg cttcaatcgc atagacaatg ccctttttct caatcaaccg
     4741 ggcaatggtc attacgtgta cccgttcact ctggagaact ttcttggggg agcctgtgag
     4801 gttttggcaa ttaataccag agggcaaaac gacaatcttc ccctcatcac atccttgctg
     4861 gacaatgaga tttttcagag attggctcac cggcaaaaag aggtcccctt ccctaaataa
     4921 tgttttatac aaatcggggc attcaaagac catttttgat gcatcaaatc ctcgaaaaga
     4981 agtcaccact ttagccgtcg gggcagccac ctgcttaata tataatcctt gcaggccatc
     5041 cgtcccaaat tgacaatgga taatgtcata gggtcctttt cctaaaaatg gaataatttt
     5101 atatagaagt gttaatgatg aagctgccct tccatatcga aaaacattta aggatcgcac
     5161 tattgggatg ggatgtttta ggatatattt aaggataaga tacaatcccc tggcgaatct
     5221 tcgagtttta tttttcggta taacttgata gatcgtacgt tcccgcaatt tatattttct
     5281 caggctttct ggacttggga gatcatccct tggtttcgcg gcaaaaatgt ttacctcaag
     5341 cccagcttct attattccta caatttgact ttgcacaaat gtttcagtgg cagagggaaa
     5401 ctggctcact atataggcaa ttttcatcct tcatcctcta cctttttttc ggttacgttt
     5461 tgcaaaaaac caatttggga atcccaatcc atttgcggac cctggccatg caatacagca
     5521 tcgctttaac gcttatttga ctttcttcga ggctctcgca ccaaataaag gatattctcc
     5581 ctcttggcaa tacctgatag aactttccca ttcccttgct caacgtcttg cataaaaccc
     5641 aagattgacc ggaggaaata tccagtaggt ccttatagca gttttcaatt ttacttaagg
     5701 agagattgga ttacgtgtat aaatatttcc tttgcacatc ctatttttct cgcacaagtt
     5761 ccgacgcata ataatcaact gaataccctc cagttttctg acggatagct tcttttacac
     5821 gttctatttc ttcatgatct attttttcaa tatatctatc gtacatatag aggacatcca
     5881 taaattcaat gtaatagggg ctggactcaa gggcattggt taaaccgaat tggaagagaa
     5941 atttcctgag aaaattaaaa ttgtttttta tgtaagggac taaccacgaa agtggcattt
     6001 gctgatacaa ggagaggaaa tctttaagat tggtaatttg atattcttcg gaaaaacaac
     6061 tgatggttaa atcccgatca atccatccgc cgggataaac aagccgggga taatgctgtc
     6121 tcagaaccca aaaaaccttt cgtatgcttc ttaccttcac ggcatgggcc actacttcaa
     6181 ccttggaacg gggctttttt cgtgatttca tccacatcac tataaacatt ctatacagtg
     6241 cctctgccgg ttgatgcgct aatactcttt gtagcgcacg taaccctttt tcggacaatt
     6301 cggaatacat tttgttttgg ggaatcacat ggctaaggcg attcaatacc gtcaatgcct
     6361 gcacatgcgt attgatacag agcgaattgc cgggagattt gccaaaaatg gcagaatgaa
     6421 aatgtgcatg tttgtccttg tgttcattgg tatcatgtaa gaaccaaaga ctcccgtcat
     6481 caagctgttc catcaggtgc tcggtcacaa atgccaatcc acgctctgcg gcttctaccc
     6541 ataatgactc acccgttttt tcatgttgcg aaatgagtaa atgaattccg tcaagatgga
     6601 atcgggcatg cgtttcaatt tcatcggtcc aaaaaccatg cccccactcc ccttttggcg
     6661 acaggtctaa aaaaacagaa tatgcaatga cattttgcat gatgtcatac agttttttac
     6721 cggtctcttt atataaaaat ccaaagtaac tccaccaggc aaatgcacat tgctgacatc
     6781 taaatcgctt cttaaggtct ttatgtccac gaggatcata gatacttccg ttgatcaaat
     6841 acctccagat atctgatggc ttgctagcaa aaaaccaatc tgtttttcga tacaaacatc
     6901 gttcagtcat atttaaagat ttgatttccc caataaactc cccacaggca tcttcgataa
     6961 tcataaacgg gacaatagct tgctctcctg ggattcctga aaggaatagg tgcaattgaa
     7021 tacctgaatt cacttcccaa tggcagggga ggaacgtgtc ctctggctct gaaattcgtt
     7081 gggattggtc aaaatgcaat ttcgcacaag cagcaggcag ccaaaccgta tatatttttt
     7141 gttcatattg aacggttaac agcattccaa attcattccc atgtaaaaca ttcaagaccg
     7201 tggctgcgct tgaaaatctt atgtgggaag ccccctcctc tgaacagaca cgaaggctgt
     7261 ggatactcac cccccgcttc acatgaacat ccaccaattg ggaacaaaat tcatcaaatg
     7321 catactcaac acgttcaaag ccattagaag cgaaaatccg ctcccctgtt gcctttccaa
     7381 atagtatatc tgttgaataa actttacttt ttgaattcat ggtactcctt gctcaatggg
     7441 agactcgata ggtaggaacc agccacgtta gtcccaccgc taagcctcat acccaaaaga
     7501 gcctatcagt tgttttattt tccttttacc tgctttccat tcatcaagaa tggcatattt
     7561 caaaaaccgg ctaatagaaa ataaataccc acatttcctg aaaagcagca acaaggcctt
     7621 taaacggata ctcacgacct tctctttttt aaccagatta taattatgcc taaagatata
     7681 attattccct gtctttctta tttttaccac catattcttt tcagaaacta actgacacat
     7741 cgtatctacc aataataatg actcttcaca ccactcttta gttttcacag atacggtcaa
     7801 ttcattggga tgtttccgat tacttgcctt actttctcga atatcctttc ggccatagtg
     7861 ggaggataat ttggctattg ccatgacatc caagagtaaa ttgtgttttg aatgcatgcc
     7921 accgatctct ctcaaccttt tagtattcaa taaggtacta cagagataaa tacccgtctc
     7981 aaaggataaa tacgccaaaa aaaattcttc cgtggaaagt ccaccaaccg tattgagcca
     8041 ctcccctatc tgctgcccat ctgggccaat atagcgagtt cctgttcgga taactccaat
     8101 atccatgaat gaatagttaa tcgtttccaa gcacacatcc acaaaatcct catcaataag
     8161 atcatcatcc tgtaatagta aaaaataatc cccccgtgct tgttctatac aaaatttaaa
     8221 attattgtaa tgcccgatat tttgactgtg ctttacatat cgaatgcgag gatcatcaaa
     8281 ttcttttacc actttttcgg tttgatcaat cgaacaatta tctgaaacga taatttctac
     8341 attttgatag gattgatctt tcgcgctttg aagagtgtgc ttcaggtaag tgtctgctcg
     8401 attgtaggtc ggaatagcaa tcgtgactaa tggcttttcc ggattaaaca tattaaacct
     8461 cttcaaagaa ataaaaactt atatcccagc taaagatagg ctcaaatgcc atttgggatg
     8521 caatcttagg cgaaaacgcc tcactcgcca tgcccgtgga ttgcggcgga aatgcatccg
     8581 aaaatttgga ttaaactgtc tttcagaata tcatcctgat taacaaggaa ggatcccgct
     8641 gaatcctgaa attttcgggt gaacagagat tcttccccaa tgactcaggc tgccaggctt
     8701 gcgcaacatt atcgttcaac tattagtttt tttgcttttt catgaactct ttcacggcta
     8761 atgataatgt ttattaatcc agagggataa actgagcaga ggccatagcg cccatgaata
     8821 atcgctccga ccattgagat gttgatcata cagctgtcgg agagctttct tgtttacttc
     8881 caaccccaac atttcatccc gtttcatgag gctttcctca accatttcac ggagtgaggt
     8941 acgcaaccat tcacccatag gcacctcaaa accccgcttc ccttgagtct gttgtttggt
     9001 atatttggat aagatctgac gtaatatcat ctttcctgtc ttttgttcca cattaaggca
     9061 ctgatgccag tccgtttgtg ccgctatctc aataacctcc ttatccagga gggggacccg
     9121 aacttctaat gaatgaaaca tacttgcccg gtccactttc atcaacacat agggcaaatg
     9181 acattcatat tcgcttatac gggaatattg tgccgcacga tctggatcgc aactatggta
     9241 ttcaaatgct ttataggcat ttggccagga tggcagcgaa ggaaaaattt gtttcaacca
     9301 ttgctcggaa agatgggtga gcatgagacg atgatagttt ccgagggagc gttgatttag
     9361 atggttataa tttccatatt gcagaactct tttaaatcct cgccagaccc aacgacacca
     9421 gaatgggtac cgaaaatcac cggcattcct caccaaggaa ccaaagcggc ccgtatagcc
     9481 ccaaaacaat tcatcaccgc catcaccgga aagcatgact ttgtaatttt gactggccag
     9541 gcgggatacc atcatggtgg ggaagagaga ataatctcca aacggctctc cacagctttc
     9601 gatcacatcc tccaaaaaat ccagcgcttt ggcaggatcc atacattcaa tcctatgctg
     9661 gactccaaaa tccctggcat atgccatggc atcctgtgtt tcgtcagtag cttcttctcc
     9721 ggttccgatt gtgaaaactt caatgggcgc tgcactcacc gatctcattt tcgcgacgac
     9781 cagaggtgag tcgatgccac cggaaaggaa tgctcccacc gggacatcac taattagatg
     9841 ccgtttcaca gcccctgtga ttgccg
//
LOCUS       DAORAQ010000095         9784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1112_length_9784_cov_62.577141, whole genome shotgun sequence.
ACCESSION   DAORAQ010000095 DAORAQ010000000
VERSION     DAORAQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1112_length_9784_cov_62.577141"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..1060)
                     /locus_tag="PKM72_09185"
     CDS             complement(350..1060)
                     /locus_tag="PKM72_09185"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNP61000.1"
                     /translation="MKMNVHRTTLMLVILLFLLQGCAATQQRRDVVKTGFLSASEQSM
                     LTEGKGDHEALLHYINPDADWRSYDRIILDSVAVWKDKETKDVSPEDLQMLTDFAYSH
                     LHEALTKDYAIVGLPGPGVMRATFAITEAEASSPGADVVTSIIPQTRILTGVKGLVVG
                     GKPGFVGTAGMEVKITDAQTGKILAMAVDRRGGTKDLSGMTNEWNDVEKAYIYWAAAS
                     RYRLCMLRGESNCEKPEA"
     gene            1518..2870
                     /locus_tag="PKM72_09190"
     CDS             1518..2870
                     /locus_tag="PKM72_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP61001.1"
                     /translation="MTRGRATSYPYPVRDTRGWTWCGEAWFLTLVMIIAGVLSLNPAV
                     AATGEGSLERQNPTVLRLSLDDAIALFLRQNLDLLQTKYGIDTAKAKRITAGLFPNPE
                     LSINTLSAYTQDCNLSKCGGIMPVISQLFLVAGKRGFRVESAEFGTQSAEAGFEDTLR
                     QLSFAVKDAYYRVQVGHRLLEDDEQRSSRINRAIEKLGGTLAKTQNPEDLIRLQIRAV
                     KTHANIIRDIQQIDSDRSDLLLLLALSPETELELTTDLTFEPIVPDIVALKKRVEDAR
                     PDLRAKRLLLAKRRSESKLAIANQYPDVTVDLGYNVQGPQGPDNQQQWTINLGMPIPV
                     FDRNQGGIKEAAATVHIAEADLHKTLNEVHHQINTVYRHLGQSRLLVETYRAGAFEAS
                     EALFNRVEQDFHSGKTTILHLLDAFQAKIDIDKAYINALYEYQRDILLLESAAAQPVS
                     "
     gene            2982..4250
                     /locus_tag="PKM72_09195"
     CDS             2982..4250
                     /locus_tag="PKM72_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP61002.1"
                     /translation="MGFLGWLELGSVGSVMAGEQEDRPAGQKPAVQRLSLDDAIVLFL
                     RQNLDLLQTKYGIDTAKAKRITAGLFPNPEFSINTLSSYTQNCELSDCGAISSVLSQL
                     FVVAGKRGFRIESAEFGMQSAEAGFEDSLRQLSFALKDAYYRVQVARRHLAFDQETRD
                     LLSRVVEKMTDELQGMGNYKDLTRLKLQMVDAQYELIRDVQRIDSGTADLLLLLSLSP
                     ETKLDLTTDLTFQSIAPDMNALKTLVEETRPDVRAKRLLYAKRRAELKLAFAIQYPDV
                     TVDLGFMVQGSRGPDNQQQWTFNVGMPLPVFDRNQGGIQEAATVVQIAEADLQQTLNE
                     AHAQVDLAHRHLVQSRRLVEAYRARALNAAQSLFDTVKKSYEAGNSEILSFLDAHRMR
                     NDIQEAYLDALYQYQRDILLLESAAGQTIS"
     gene            4381..5142
                     /locus_tag="PKM72_09200"
     CDS             4381..5142
                     /locus_tag="PKM72_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNP61003.1"
                     /translation="MGRIIAITNQKGGSGKTTTAVNLAATLGELKRRVLLVDLDPQAS
                     ASNWYGISEEGKGLFEAFTESKNLKDLIQSTCVPRVDMIPSSSWLVGVDKALAGEVGA
                     EMILNRHLADLSDHWDYILLDCSPTLGIVTINALVAAREILIPVETHVMALRGLAHLL
                     QTVETIRVRLNSALAISGILACRVDARTRHALEVVAQLRERFKKQVFKVVVRESIRLA
                     ECPSFGKPVTLYDPQGNGAKDYRALAKEVIRQERV"
     gene            5192..5605
                     /locus_tag="PKM72_09205"
     CDS             5192..5605
                     /locus_tag="PKM72_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61004.1"
                     /translation="MTKRRTIGTNPLDEIIPNPLGDAIPGQQEGPDQFALQESRKQGI
                     AGKTAGQHSVPDRVQHDKKEVRFLHLVSKDEGLKGNFKSSQAVPAAAPQVRIEMHPAT
                     GHLSERLVELEEENLCLKWALGLILAPFVLLAFLG"
     gene            5855..6817
                     /locus_tag="PKM72_09210"
     CDS             5855..6817
                     /locus_tag="PKM72_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNP61005.1"
                     /translation="MKIHCFRLQEKGLLQLTEERPTASWLEDGIVRLLDVDYPDVTKD
                     LRELLSPLNLEGALLDKIETQAEGVCVETFLNALFLRYPRPAIHDFKDLYISMICVRE
                     TLIILHNGMPLPLEQIFARIDRFGGIGTTAITDLLLHILLLVINRDIELYQQTRQLIG
                     ELSNNFDHCLNTEVLNDIQNLTNQVSRLQSTTEDRLIFLGGLLTLKSPLLELHDVRLY
                     FKEAMNELMQMQRWLERLEGRLQGLSQYYNLSLQNSTNNRLQVLTVISAIFMPLTLIA
                     GIYGMNFTNMPGLQMHYGYFIILGFMVLLGGGLGIFFYVRGWFH"
     gene            complement(6889..7224)
                     /locus_tag="PKM72_09215"
     CDS             complement(6889..7224)
                     /locus_tag="PKM72_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61006.1"
                     /translation="MGAEEEELKTKIRQLVNERFDGDFRKGFDHYDKIDGEDFKIGSS
                     ALEQLLADAKVGNFLTRKLWMEGVLKVLDKDKDGAISWDEFESILDDSPQTKASEATE
                     PGDERSPDK"
     gene            complement(7238..7675)
                     /locus_tag="PKM72_09220"
     CDS             complement(7238..7675)
                     /locus_tag="PKM72_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61007.1"
                     /translation="MMNSNQQRQLSLSRFWVILTIWWGVGLFACASQDLVLTGSWVSA
                     EMTHPSPFFTETLGDDRHKPITLIFDQAGGFVWLAPQEVCHFGTYRVQDHTLLLTEPE
                     GETVILRYAFIGHQLQLKSPDGFMFDFRKVPHQADADAKPCKR"
     gene            complement(7717..8337)
                     /locus_tag="PKM72_09225"
     CDS             complement(7717..8337)
                     /locus_tag="PKM72_09225"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNP61008.1"
                     /translation="MRNWSRGIFLGHLTSLERLNALTDGVYAIVITLLVLDLKVPETP
                     GLTTGQLLSDLQEQIPNFAAWILSFYMAAFLWMRNFWILKHIGKCDEKTFWLNFVHLM
                     FVSLIPYAASLLGHYEEDAVAVIIFSSTLGLASFSLLILHQYVAAKRDWHLEGAPKLW
                     TNPNLWAMYPAPLFAIGSILISFNSVNGAIAFWFLAPLWTRLFSQK"
     gene            8775..9491
                     /locus_tag="PKM72_09230"
     CDS             8775..9491
                     /locus_tag="PKM72_09230"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HNP61009.1"
                     /translation="MTNAEQRVHEHIVTRVCGGSFGGCGATREARSVEQSGFLGDVYS
                     QMHAGKDDEALLVYRNEKADWKSYEKVMIAPVTVWVGTKDSQLEYVSPLDRQRLANDL
                     WGKLHEALQTDYEIVDEPGPGVLLIKAAITEGEASWPVIDTVSTIVPQMHVLSELKYL
                     ATGTASFVGKASGEIKVTDGQTGELLLAAADRRAGGKRLEGVTHSWDDVEEVYRYWAE
                     QSRWRLCKLRDAPDCVEPES"
BASE COUNT         2386 a         2407 c         2543 g         2448 t
ORIGIN      
        1 cattcccgac aacctttgct atctaggctt tcctttggat gggtacccgc ctgaagctgc
       61 gggaatgacg gaaacaggat aaatacctgc cattgactac agggcaacct ccatctccgg
      121 agacatgaac cagggtgttc tgtcagtgtt ggtaaaatct caattgtttc tgacggtgtc
      181 tggcaactgc atgtgctgtt tactttggga tcaatgaagt aggaaagtcc gagcgttgca
      241 caaacgtatc gtcctgaagg caccctgcac ccaaaacgaa caaccctagt ttttccagat
      301 cctcgcaagt tcccgcccaa cttggactcg gtcggtatgc cggtatcttc tatgcttcag
      361 gtttttcaca attcgattca ccgcgaagca tgcacaaccg atagcgagaa gcggccgccc
      421 aatagatgta cgccttctcc acatcattcc attcgttggt cattccgctg agatctttgg
      481 tccccccgcg ccggtcgacg gccatcgcca gaatctttcc ggtctgggcg tcagtaatct
      541 tgacctccat gccggccgtt cccacaaagc ccggcttgcc gcccacgaca aggcctttga
      601 cgccggtcag gattctggtt tgaggaataa tcgacgtcac cacatccgcc cccggacttg
      661 aagcctccgc ttcggtaatg gcaaaggttg cacgcatgac gccaggtccg ggtaggccca
      721 caatggcata gtctttggtc aaggcctcat ggaggtggct ataggcaaaa tcggtcagca
      781 tttgaagatc ctcgggagag acatctttcg tttctttgtc tttccagacc gccacggaat
      841 caagaattat cctgtcgtag gaacgccagt ctgcatctgg gttgatgtag tgaagcaggg
      901 cctcgtggtc acccttccct tctgtgagca tggattgttc actcgcactc agaaaaccgg
      961 ttttgaccac gtctcgccgt tgttgcgtgg ccgcgcatcc ttgtaagagg aaaagaagga
     1021 tgaccaacat cagggttgtg cgatgaacat tcatcttcat gaatttattt ctcctttatg
     1081 tgtaaggaaa attagtacgt tgtacagtta agaagaatat tagcttacca attttaggga
     1141 aacatcccaa ataaggatat tcagagaacg tatggttcat gtcgttcaat ggacgtcgct
     1201 accaagcctc tgaatggcca acgtgttaat ccgggagtaa cagtcaataa ttacaacgga
     1261 cgcaaaagaa atgttggcag taaagaaagt ccttttgggt ctatggcatg tggcaacgga
     1321 ctgaatattt gagctatgct ggcgccatgt atttatgggt atattgggtt ggaccagaac
     1381 gacagcacgg gttagcctgc ccgtgaaatc tgcccttgca tatatgtcct gtcgcgcgtc
     1441 gacgcgcagg ggggaagcgt ggtgcaggca ctagaatgga tctttggctt ggtgagttgt
     1501 caggagggag gaaacgcatg accagaggtc gggctacatc ctacccctat ccggtgagag
     1561 atactcgggg atggacatgg tgcggggagg catggtttct cacgttggtg atgatcatcg
     1621 cgggggtgtt gagtctgaac ccagcggtgg ctgcaacagg ggaggggagc ctggagaggc
     1681 agaaccccac ggtgctgcgt ctgagtttgg atgatgcgat tgccttgttt ctccgtcaaa
     1741 atctggatct tcttcaaacc aaatatggca ttgataccgc caaggccaaa cgaattaccg
     1801 ccggcctctt cccgaatccc gaactctcca tcaataccct cagcgcgtac acccaggatt
     1861 gtaacctgag taaatgcgga ggaattatgc ccgtgatcag tcaactgttt ctggtggcgg
     1921 gtaaacgcgg attccgtgtt gaaagtgcgg agttcgggac gcaatcagcc gaagccggat
     1981 tcgaagatac gcttcgacaa ctcagttttg ccgtgaagga tgcctattat cgggttcaag
     2041 tggggcatcg ccttctggag gatgatgaac agaggtccag ccggatcaac cgtgctatcg
     2101 agaaactagg tggtacattg gcaaagacac aaaatccgga ggatttaatc cgccttcaaa
     2161 ttcgagcggt taaaacacat gctaatatta tccgggatat tcaacagatt gattcggacc
     2221 gatccgatct ccttttgctc ctggccctct caccggaaac ggaattagaa ctgacgacgg
     2281 acctgacgtt cgagccgatc gtccccgata ttgtggcttt gaagaagcgc gtcgaggatg
     2341 ccaggccgga tcttcgcgcc aagcgtctgc tgttggccaa gcgccgttca gaatcgaagc
     2401 tggcgatagc caaccagtac ccggatgtga cggtggacct cgggtacaat gtccagggac
     2461 ctcaaggacc tgacaaccag caacaatgga ccatcaattt gggcatgcct attcccgtgt
     2521 tcgacagaaa tcaaggaggc atcaaggaag ccgccgccac agtgcatatt gcagaagccg
     2581 acctgcacaa gaccctgaat gaagttcatc accaaataaa cacggtctat cggcatttag
     2641 gccaaagccg tctgctggtc gagacctacc gcgcgggtgc cttcgaggcc agcgaagctt
     2701 tgttcaatag ggtggagcag gacttccatt cgggaaagac gacgattctg catctgctcg
     2761 atgcctttca ggctaaaata gatattgata aggcatacat caacgctctt tacgaatatc
     2821 aacgcgatat cctgcttctc gagagtgcgg ctgctcagcc agtcagctaa agatcccggc
     2881 ctatgatgtg ttcccaatct tccttttgtc ctttttcacg cgttcgcgac cacgcaaaac
     2941 cagtgccggt gattcggttc ttcctgaagt catgcatcat catgggcttt cttggctggc
     3001 tggagttggg aagcgttggt agcgtcatgg cgggagaaca ggaagacagg cctgcaggac
     3061 agaagcccgc agtccagcgt ctgagtctgg atgatgcgat tgtcttgttt ctccgtcaaa
     3121 atctggatct tcttcaaacc aaatatggca ttgataccgc caaggccaaa cgaattaccg
     3181 ccggcctctt tccgaacccg gaattttcta tcaataccct gagttcctac acccaaaatt
     3241 gcgaactctc tgattgtgga gcgattagct ccgtcctgag ccagctgttt gtcgtggccg
     3301 gcaaacgcgg attccgtatt gaaagtgcgg agttcggcat gcaatcagcc gaagccggat
     3361 ttgaggactc gcttcgacag ctcagtttcg ccctgaagga tgcgtattac cgtgttcagg
     3421 tcgcacgtcg tcatttggct ttcgatcaag agactcgtga tctcctctct cgtgtggtgg
     3481 aaaaaatgac ggatgagtta caagggatgg gcaactacaa agaccttacc cggctcaaac
     3541 ttcaaatggt ggatgcacaa tatgagttaa tccgtgatgt tcagcgaatc gattccggca
     3601 ccgccgacct cctcttgctc ttatccctct cccccgaaac taaattggac ctcacaacgg
     3661 acctcacgtt ccagtcgatc gcccctgata tgaatgcatt gaaaacactg gttgaagaga
     3721 cgcgaccgga cgttcgggcc aagcgtcttt tgtacgcgaa gcgtcgcgcc gaattgaagc
     3781 ttgctttcgc cattcaatac ccggatgtga cggtggacct cgggttcatg gtccagggat
     3841 ccaggggacc tgacaaccaa cagcaatgga ccttcaacgt gggtatgccc cttcccgtgt
     3901 tcgaccggaa tcagggcggc atacaggaag ctgccaccgt cgttcaaatc gccgaggccg
     3961 atttgcagca gacgctcaat gaggcgcatg ctcaagttga tttggcacat cgtcatctgg
     4021 tccaaagtcg tcggttagtg gaagcctacc gggccagggc gttgaatgcc gcgcagtccc
     4081 tgttcgatac cgtgaagaaa tcttacgaag ccgggaacag cgaaattctg tccttccttg
     4141 atgcccaccg gatgagaaac gatattcaag aggcatacct tgatgctctc taccagtatc
     4201 aacgtgatat ccttctcctc gaaagtgcgg ccggacaaac cattagctga gtaggatgga
     4261 gtgaccaatc ccagttctcc ttgacttttt agccggcttt gctgaaactc gtccatgtct
     4321 tttttatgtt ccactgaccg tttcctgatc caataacagg ggcattccct ttctcaagct
     4381 atgggtcgca tcattgccat caccaatcaa aagggcggaa gcgggaagac gacaaccgcc
     4441 gtaaatcttg ccgcgacact tggcgaactc aagcgtcgag ttctgctggt ggacctcgat
     4501 ccccaggcct cagcctccaa ctggtatggg atttccgaag agggaaaggg gctcttcgag
     4561 gcctttacgg aatccaaaaa tctcaaggat ctcattcaat cgacctgtgt gcctcgggtt
     4621 gacatgatcc cctcttcatc ctggctggtg ggggtcgata aggcgttggc cggggaagtc
     4681 ggagcagaga tgattctcaa tcgtcatctc gctgacctgt ctgatcactg ggattatatt
     4741 cttcttgatt gttctcccac gcttggcatt gtgaccatca atgcgttggt agccgccaga
     4801 gaaattctca ttcccgtaga gacccatgtg atggccttgc gtggattggc tcatctgctt
     4861 caaacggtgg agacgattcg tgtcaggtta aactcggctt tagcaatttc cgggattcta
     4921 gcctgtcggg tcgatgctcg cacgcggcat gcattagaag tggtggccca actccgtgag
     4981 cgttttaaaa agcaagtgtt taaagtggtt gtcagggaaa gtattcggtt ggcggaatgt
     5041 ccatcattcg ggaaacccgt gacattgtat gatccacagg ggaatggcgc aaaagactat
     5101 cgggctttgg caaaagaggt aatcaggcag gaacgggtgt gactgattgg gaagtattca
     5161 aggtaccctt tttttttagg agaggtcgcg tatgacgaaa cgacgtacaa ttggaacaaa
     5221 tccactggat gagatcattc cgaatccatt gggagatgcg atacctggtc aacaggaagg
     5281 tccggaccaa ttcgcgcttc aggaatcgcg aaaacaagga attgcaggca aaaccgccgg
     5341 tcagcattct gtgcccgatc gcgtgcaaca tgacaaaaaa gaggtgagat tccttcatct
     5401 ggtttcaaag gatgagggtc taaaagggaa tttcaagagc tcccaagcgg ttcctgcagc
     5461 agcacctcaa gtaagaattg agatgcatcc ggcgaccggt catttatctg aacgccttgt
     5521 tgaactcgaa gaggaaaacc tttgtctgaa gtgggcattg ggattgattc tcgcgccctt
     5581 tgtcctttta gcctttttgg gctagatccg attcagtcag atgacacctc tatgccgggt
     5641 aaaaggggcg ggtgcctttt ctggttttcc tcaagaagaa tcttgccgga cctttttgaa
     5701 attcccctga gaaacctgct taaggtcatg ggcagtttag tcacacacac atcccccatt
     5761 tttatgcgtt ctgttttaaa caggatcaac ttcaccgtga tcttggactt ccaaggtctt
     5821 tgataagatt catcagcatc ggaaggtgct cctcatgaaa atccattgct tccgactcca
     5881 agaaaagggt ttgcttcaac tcaccgagga aagacctacg gcatcatggc ttgaggatgg
     5941 aatcgtccgt ttattggatg tggattaccc agatgttacc aaagatctgc gtgaactcct
     6001 ttcccccctg aatctggaag gcgctctgct ggacaagatc gaaacccaag ccgagggtgt
     6061 ctgtgttgaa acattcttga acgccttgtt tcttcgatac ccacgcccgg ccattcatga
     6121 ttttaaggat ctctatattt cgatgatctg tgtgcgagaa accctcatta ttctccacaa
     6181 tggaatgccc ttgcccctgg aacaaatttt cgcgaggatc gaccggttcg gagggatcgg
     6241 aaccactgcc atcacggacc ttcttctcca tatcctgtta ttggtcatta atcgtgatat
     6301 cgaattgtat cagcaaactc gacaattgat cggggaattg tcaaataact ttgatcattg
     6361 tcttaacacc gaagtactca atgatattca gaatctgacc aatcaggtta gccgtctcca
     6421 atcaacgact gaagatcgtc tcattttctt gggaggctta ctcacgttga aatcccctct
     6481 attggaattg catgacgtgc gattgtattt taaagaggcc atgaacgaat taatgcaaat
     6541 gcagcgttgg ttggaacgtc tggagggaag gctacagggt ttatctcaat actacaattt
     6601 gtcgctgcag aatagcacca acaatcgtct tcaagtcctg acggtcattt cggccatttt
     6661 tatgccactc acccttattg ccggtattta cggcatgaac ttcacaaata tgcccggact
     6721 tcagatgcac tacggctact ttatcattct cggtttcatg gttttgctgg gtgggggatt
     6781 gggaatattt ttttatgtac ggggctggtt tcattaaagg cccctttcga ttggcgtgac
     6841 attcttgaaa gagtggttgg gaggcctttt cattcccaat ggtcaaccct atttgtcagg
     6901 ggatcgctca tctcccggtt ccgtggcctc actggccttg gtttgcgggc tgtcatcaag
     6961 aatcgattca aactcatccc aggaaatggc accgtccttg tccttgtcga gtaccttcag
     7021 gacgccttcc atccatagtt ttcgggtcag gaagtttccg accttggcat cggctaagag
     7081 ttgctccaag gcggatgagc cgatcttgaa atcctctcca tcgattttat cgtagtggtc
     7141 gaagccttta cgaaagtcac cgtcaaaccg ttcattgacc agttgacgaa tttttgtttt
     7201 caactcttcc tcttcggctc ccactggtat ctcctttcta tcgtttgcag ggcttggcat
     7261 ccgcatcggc ttggtgcggg acctttcgaa aatcgaacat aaaaccatcc gggcttttca
     7321 gttggagttg atgaccaatg aaggcatacc ggaggatgac tgtttcacct tccggctcag
     7381 tcaaaaggag ggtgtggtct tggacacgat aggttccgaa gtggcaaacc tcttgggggg
     7441 caagccacac aaacccaccg gcctggtcaa agataagggt gataggtttg tgccggtcgt
     7501 cgcccagagt ctcagtaaag aagggcgacg gatgtgtcat ttctgcactc acccacgacc
     7561 ctgtcaatac caggtcctgg gaggcacagg caaaaaggcc gaccccccac catatggtta
     7621 atatgaccca gaatcggctc agtgatagct gtctctgttg attggaattc atcatctttt
     7681 tctctgaccg gatgtgtgtt cttgttctaa gtccgattac ttctgtgaga aaagacgtgt
     7741 ccagagcggg gcgaggaacc agaaggcaat cgctccattg acgctgttga aagagatgag
     7801 gatagaacct atcgcgaaaa gtggtgcggg gtacatggcc cacaagttcg ggttcgtcca
     7861 gagcttggga gcaccctcca gatgccaatc gcgtttggcg gccacatact ggtggaggat
     7921 aagtaaagaa aaactggcca gaccaagagt tgaggagaaa atgatgaccg caacggcatc
     7981 ttcttcgtaa tgtccgagca acgaggccgc gtacgggatg agggatacaa acatcagatg
     8041 aacaaagttt aaccagaatg tcttttcatc gcatttccca atgtgcttga gaatccaaaa
     8101 attccgcatc cacagaaacg ccgccatata aaaactgagt atccaggcag caaagtttgg
     8161 gatctgctcc tgcagatcgg aaagaagctg tcctgtggtc agtccaggag tttccggcac
     8221 cttcagatcc aacaccagaa gcgtgatcac aattgcgtac acaccgtcag tgagggcatt
     8281 gagccgttcc agtgaggtga gatggcctaa aaaaatccct ctgctccaat tcctcataag
     8341 cgcgtttggt tcatgaacat gagtggattc ctacacccct ctggtcttga gcatccaagg
     8401 gtgagtgatg aatttgggga cgttgaggtg gttgcccggg tgatagaatc ggtgaaaatt
     8461 ataaacggaa actggaaaat attaccccaa agaatactac gagtgccact ccggccgtcc
     8521 cccggttgta tggatactcg caaaacgccg cccaatagat aaaatggagg gtcttgagaa
     8581 caacataacg aagagatagc ttcaagtaat cagaaccggt acagattggg ggacagggga
     8641 ggaggtcgat atgaccatgt ggaaaagaaa ctggaacatg tcattccccc tgacaatctg
     8701 ataaattatc ctcgcggtta aagatatggc atgttgtttt ttccggctta tcattccaaa
     8761 ttcaagaaag agatttgacg aatgcggaac aacgtgttca cgaacacatt gttactcgtg
     8821 tgtgtggtgg gtcgttcggt ggctgtggcg ccactcgcga agcccggtcg gtggaacagt
     8881 cgggcttcct gggtgatgtg tattcccaga tgcatgcagg caaggacgat gaagccctgc
     8941 tggtttatcg aaatgaaaag gccgactgga agtcgtatga aaaggtgatg attgccccgg
     9001 tgacggtttg ggtggggacg aaagattccc aacttgagta cgtgtccccg ctggatcgcc
     9061 agcgtctggc aaacgatctt tggggtaagc tgcatgaggc tcttcaaacc gattacgaaa
     9121 tcgttgatga gccgggtcct ggtgtgctgc tcatcaaagc ggctattacg gagggggaag
     9181 cctcctggcc ggttatcgat actgtttcga ccattgtgcc gcaaatgcac gtcctgagtg
     9241 aattgaaata tctggccacg ggcacggctt cctttgtagg gaaagccagc ggggagatca
     9301 aggtgactga tggccaaaca ggagagctgc tcttagctgc ggctgatcgt cgagcagggg
     9361 gcaagagact ggagggcgtg acacattcct gggacgatgt ggaagaggtc tatcgctatt
     9421 gggcagagca aagccgatgg cggttgtgca aattacgtga tgcaccagat tgcgtcgaac
     9481 cagaatcatg agaaggcggt tgatatgtga tcattggcct tttggcgtca gaacgtctaa
     9541 cgtcctgtcc tgatgactga ctctcaactg tcctgatccc atacctcatt cttcgcaggt
     9601 cggagagtta ttcgaaaacg ttcccactca acaacgttcc tcgctttcct cccttgaaca
     9661 ggcgtggtca gctatgctat gccttccgtg ttaatgcttt ttgtcaatta ctagggagag
     9721 gcccatgacc aaggctattc acagaatcgc acgaacactg gtcttcatgg tccttatcgt
     9781 tccg
//
LOCUS       DAORAQ010000096         9580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1136_length_9580_cov_66.476325, whole genome shotgun sequence.
ACCESSION   DAORAQ010000096 DAORAQ010000000
VERSION     DAORAQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1136_length_9580_cov_66.476325"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKM72_09235"
     CDS             <1..425
                     /locus_tag="PKM72_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61010.1"
                     /translation="YGVGAVVGVKNEPLAWETAENRARAELAKTFRTYTAYLMQDYAA
                     STSGGNFTKSTEEQNIERAVKTFSAVTLNGVRPIDRYKDDETSTYYVLTKLSFAEMKE
                     ALQQAQELDEEVRDFVRKNAEKAFKKLEEEEAKRPGGL"
     gene            483..1106
                     /locus_tag="PKM72_09240"
     CDS             483..1106
                     /locus_tag="PKM72_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNP61011.1"
                     /translation="MLPLLRMIKSCAIVVPCLSLLLLGGCGKEKKVTRVDPGLVTDFS
                     GRWNDTDSKLVAEAMMKEMVSQPWLENFSSQYNRVPTVIVGTILNKSQEHINVGTFIT
                     DLEREMTNSQRVLFVASKSDREEIRQERQEQAVHAREDTQKRPGQELGADFMMKGTIS
                     TIVDESDGVKGIYYQVDLDLINLETNVKSWYGQKKIKKVVERKRLLF"
     gene            1148..2668
                     /locus_tag="PKM72_09245"
     CDS             1148..2668
                     /locus_tag="PKM72_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61012.1"
                     /translation="MRNPHSAFPQRFKFFPISPFSCRLHEANCWSWWRLGLLGFVFLL
                     AGCNGTFSHYKEVDHSLKAGNPDKAVTLIQEAEDDYGRKNRLLYLMDEGMVLHLAGRY
                     EESNTVLEEAYLLVEELYTTRLRDQASAILINETQLPFEGEPFEHVLINVIMGLNYAL
                     LQNWNEALVEARRIDHRLNVLSDRVEGDAYKEDPFARYLTGVLYEIAGDLNNAYVAYR
                     KAETIYDETQSWSKMPLPDGLKTDLMRVADILHLSEDLEEYRTKYPEMVDEVPLPYDA
                     DMAQLIVVSYHGRSPKKEDLFIDVPISLDALALVALAKSVGGQSTRSNREGEALIYGI
                     HGQIARVALPRLVPQHTKIAYSTIQLTDGTTTFDARSQRVYDIDAVAEKNLDDEYATL
                     VLRAVARTAMKMAAAEGIGIGARSAVSKNSQDWVGLVAVILARIFVLMTEEADIRSWR
                     TLPGEIHLTRLWIPAGSYNLMVNAVDRQGRVIGQPNTDQLDFRSGEVRLLLRQYLE"
     gene            2682..3767
                     /locus_tag="PKM72_09250"
     CDS             2682..3767
                     /locus_tag="PKM72_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNP61013.1"
                     /translation="MWVKYFLPLIGALILSGCWFGNTSPPPEWILTPQNAYPADRYLV
                     GMGEGSTRDQAEKRAYAAVARIFSANVQAKSLDQESYSLTEANDQSSTHRTLRIDQET
                     QVTTTKLIENVKILDAWYRSLDQQFFVLAGLDRHHTETLLVERLSDLDATIERLVAQG
                     RSHPQKLHRIQGYKQALVLLQQRKVMNTDLRVIRASGEGISSSYTRSQIQREFMDFVA
                     KNLLISVAIEGEYHEDLERAILEGLKQEGLLGSPASSKPSEIADLAIVGQGRFWTVNL
                     PDPLFKYVRWCGDIDIYENPSHRLIGVISETGREGHVTEKEARIRASKVMQDVISKKI
                     ARLLTQSMFSAEPDSSQSQSVPNACSR"
     gene            complement(3843..4361)
                     /locus_tag="PKM72_09255"
     CDS             complement(3843..4361)
                     /locus_tag="PKM72_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61014.1"
                     /translation="MKPVLPQPPEIWVLLIVLMIPYAGNGLAQVPGEIGGMDRLEQQA
                     EYAIANGDPEGAALSIGKAALMAKILAQKEEHQQTRLLYQAEETLFRGQEQGYRAFAL
                     FERAGGQPPASKGVCQYLSHAAEKVKQSQNDLKALADFTNELLRDRQQRHIGKTQEWE
                     RLLQGLEQDLSC"
     gene            complement(4395..5498)
                     /locus_tag="PKM72_09260"
     CDS             complement(4395..5498)
                     /locus_tag="PKM72_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP61015.1"
                     /translation="MRAWATCGFITIVLLVGLFGSEGLARRTYLDADQKAKLDAIQTI
                     LIHVLALTEHGKVDGTVIEDVVRQRLEELGYTVVISRDVPYDVRVNVKCEEQKTWSGT
                     TRAGGDADQIGSPSRLWKGPACLFSYQLDGRDLGWYKEARTKFEDSGQAAQAANAPQP
                     GPYAIQELAKQIREFDFPVMLAAEWGHNTRLFKLLNNPQTPNKRKIRIFSLMPQVESQ
                     QYIPLLKELIQDPEIAEEAIVAMSSAGSEAIPILTEIFHTQTDSAIRAAAAKGLGEIA
                     SLTGDPTITPPMLAYVVENLSHIKTSSDIDFPVMTEVVWSLGKLRNAKAVAPISELEN
                     KIWVIHDNSKEMAELREATSWTYKQIEMDSQIQ"
     gene            complement(5633..6739)
                     /locus_tag="PKM72_09265"
     CDS             complement(5633..6739)
                     /locus_tag="PKM72_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP61016.1"
                     /translation="MKAWPVFLIFAMLWVDGTQSDARQISPTTEQKTKLETAQIVLVE
                     VLALTEKGRYDAQPLTATVKKRFEEMGYTVTTERSQPYDVEVKVKCEEQKTLTGTSPS
                     GGDVDLADAPDRLWKGPACLLTYFLQQNDFQWKKEVRTNFADARQAAQEAQVDDAGKY
                     AMEHLNQRLMEFDFPVLLSAEWGQIDRLLKLLDNPNTPKLRKVKILSVLSDAHAEEAL
                     PQLTKLLESTDLQQEAINALSGAGIESIPLLIDLFQTSRQSNLRAEAAKALGIIASKS
                     GDPRTIPPLAHYVSEVLPHIKTSEDINFPLLTEVVWAIGKLRWGPSIKPISELQDKVW
                     LIHDNSKEMAELREATSWAYKAIALQDAAMLQTY"
     gene            7093..7905
                     /gene="proC"
                     /locus_tag="PKM72_09270"
     CDS             7093..7905
                     /gene="proC"
                     /locus_tag="PKM72_09270"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNP61017.1"
                     /translation="MSQSPKLAFLGAGNMCEALVTGILKANLASPANISVTDISSIRL
                     EHFQQTFQVQVGSHNADEVKSADIIVLCVKPQVMDIVLSEIKDQLSPKHLVISVAAGY
                     PLARIQQHIGENVSLIRAMPNTPAVIQEGVTALAGSSGLPSQHLLLAQSIFESVGRVV
                     TVEESLMDAVTGLSGSGPAYIYLVIEALTDGGVRMGIPRTVATVLAAQTVLGAAKMVL
                     ESGEHPALLKDRVTSPGGTTIAGLQQLEIGRIRATLMNAVEAATARSHELGQ"
     gene            7970..8155
                     /locus_tag="PKM72_09275"
     CDS             7970..8155
                     /locus_tag="PKM72_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61018.1"
                     /translation="MQYWVKVIFVDNKELEFKDAIRHTISDDMEILEIDTPKEVTIIP
                     LKQIKYFSCDAGVFAKK"
     gene            8489..9229
                     /locus_tag="PKM72_09280"
     CDS             8489..9229
                     /locus_tag="PKM72_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNP61019.1"
                     /translation="MIMETIKQVGKQISLVLVLLLGMLILWELPHHDRNYLSHQERTA
                     QLGTKLPSYEVKTFLRHIETRLPSYREEFQQAEKKSGISWMLLAAMAYQESQWNHKAV
                     SPTGVRGIMMLTRSTASDLGIKNRLDPSKSIAGGARYLSYLQKRVPDNIRMPDRMFFA
                     LAAYNVGMGHINDARLLAKRLGKNSDQWEDLKSILPLLAHKEYYQDLPHRYARGWEPV
                     KYVKRIRAYRNILQQVMKREGKMSQVDI"
BASE COUNT         2424 a         2231 c         2380 g         2545 t
ORIGIN      
        1 tttatggtgt cggggcggtg gtcggtgtga aaaatgaacc actcgcttgg gaaacggctg
       61 aaaatcgcgc acgcgccgag ttggcaaaaa cctttcgcac ctatacggcc tatctcatgc
      121 aagactatgc cgcttcaact tccgggggga atttcaccaa atcaaccgaa gagcaaaaca
      181 tagaacgagc ggtcaagact ttttcagccg taaccttaaa tggagttcgc cccattgacc
      241 gttacaagga cgacgagacc tccacttatt atgtgttgac caagctgtct tttgcggaaa
      301 tgaaagaagc gttacaacaa gcgcaggaac tggacgaaga ggttcgggat tttgtgcgca
      361 agaacgctga aaaggctttt aaaaaattgg aagaggaaga agccaagcgc ccgggaggtc
      421 tttgatcctg gttaagtgac gacattttgc cttcttggtt tatcaacaac aaaggaatca
      481 ccttgctccc cttactccga atgatcaaat cttgcgcgat tgttgttccc tgcctttctc
      541 tcctcttgtt gggcggatgc ggaaaagaaa aaaaggtgac cagggtggac cccgggctcg
      601 tgaccgattt tagtggtcgg tggaatgata ccgattccaa attagttgcg gaagcgatga
      661 tgaaagaaat ggtcagccaa ccctggttgg aaaacttttc cagccagtat aatcgggtac
      721 ccacggtcat cgtcgggacc attctgaata aaagccagga acatatcaat gtgggcacct
      781 ttatcacgga tttggaacgg gagatgacca attctcaaag ggtgctattt gtggcgagta
      841 aaagtgatcg cgaagaaatt cgccaggaac ggcaggaaca agccgttcat gcccgggaag
      901 atactcagaa acgtccaggc caggaactgg gagcggattt tatgatgaag ggaaccatta
      961 gtacgattgt tgatgaatcc gatggcgtga agggaatcta ttatcaggtt gatttagatt
     1021 tgattaattt ggaaaccaat gtgaaatcct ggtacggaca gaaaaaaatc aaaaaagtcg
     1081 tcgaacggaa acgtctgctg ttttaacgca atgtcctccg gtgagccatt ttcatccctc
     1141 gtggttcttg cgtaaccctc attcagcctt tcctcaacgt tttaaatttt tccccatctc
     1201 tcctttttcc tgtcgactcc atgaggcgaa ctgctggtcg tggtggcgcc ttggtctgct
     1261 cggttttgtt tttctcctcg cgggatgcaa cggaacattt tcccattata aggaagtcga
     1321 ccacagtctc aaagcgggca accctgacaa ggccgtcacc ctgattcaag aagccgaaga
     1381 tgactatgga cgtaaaaacc ggcttcttta tctgatggac gagggaatgg tcctgcattt
     1441 ggccggtcgg tatgaagaga gcaacacggt cttggaagaa gcctatctac tggttgagga
     1501 actgtataca acacggctcc gggatcaggc ttcggcgatt ctcatcaatg agacccaact
     1561 tccttttgaa ggggaaccct ttgaacatgt tctgatcaac gtcattatgg gattgaatta
     1621 tgcgttgtta caaaattgga atgaggcatt ggtcgaagca cgcagaatcg accaccggct
     1681 caatgttttg agtgaccggg tagaaggcga tgcgtacaag gaggatccgt ttgcccgtta
     1741 tctgaccggg gtgttgtatg aaattgcagg ggatctgaat aatgcctatg tggcgtatcg
     1801 caaagcagaa accatttatg acgagactca gtcctggtcc aagatgccac tcccggatgg
     1861 tctgaagaca gacctgatgc gggtagccga cattctccat ttgtcagaag atcttgagga
     1921 gtatcgaacg aaatatcctg aaatggtcga tgaggtcccc ctaccttatg atgccgatat
     1981 ggctcaactg atcgtggtga gctaccatgg tcgaagtccc aaaaaagagg atcttttcat
     2041 agacgtgccc attagcctgg atgcgctggc gttggttgcc ttggctaaat cggtcggagg
     2101 acaaagcaca cgatcaaaca gagaaggaga ggcgctgatc tatggaatcc acgggcaaat
     2161 tgctcgtgtg gccctacctc ggctcgttcc acaacatacg aagattgcct acagcaccat
     2221 ccaactgacg gatggaacga cgaccttcga cgcgcgctcc caacgagtgt acgatattga
     2281 tgccgtcgca gaaaagaatt tggatgacga gtatgccact ctggttttga gggctgtggc
     2341 tcgaaccgcc atgaaaatgg cagcggcgga aggaatagga atcggcgccc ggtccgcggt
     2401 atccaaaaat tcacaagatt gggttgggct cgtcgcggtt attctggctc gaattttcgt
     2461 gctaatgaca gaagaagccg atatccgctc atggcggact ttgccgggag aaattcactt
     2521 aacccgcctg tggattccag cgggatccta taacctcatg gtgaacgcgg ttgatcggca
     2581 gggacgggtc attggacagc cgaataccga ccaactggat tttcggagcg gagaggttcg
     2641 gttgttactc cgacagtatt tggaataaaa ggatttttgt gatgtgggtt aaatattttc
     2701 ttcccttgat tggtgctctg atactgtccg gatgctggtt tggaaatacg tcacctcctc
     2761 ccgaatggat tctgacccca cagaatgcct atcctgccga tcggtattta gtcggaatgg
     2821 gggaagggtc tacccgtgac caggcggaaa aacgtgcgta tgcggccgtg gcacgaatat
     2881 tttctgctaa cgtccaagcc aagtccctgg atcaggaatc gtattccctg accgaagcga
     2941 atgatcaatc ttcgacgcat cggaccttgc gaattgatca ggagactcag gtgacaacaa
     3001 ccaagctcat tgaaaatgtc aagatattgg atgcctggta tagaagcctc gatcaacagt
     3061 ttttcgtcct agccggcttg gaccgacacc acacggagac cctcctggtc gaacgcctta
     3121 gtgatctgga tgccacgatt gaacgtttgg tcgcgcaagg tcgatctcat cctcaaaaac
     3181 ttcatcgcat tcaaggatat aaacaggctc ttgttcttct gcaacaacga aaggtgatga
     3241 acacggattt gcgggtcatt cgggccagtg gagagggtat atcctcatcc tatacccgtt
     3301 cacaaatcca acgagaattt atggattttg tcgccaaaaa tttgctcatc tcggtggcta
     3361 ttgagggaga ataccatgag gaccttgaac gagcgatatt ggaggggctc aagcaagaag
     3421 gcttattagg cagtcccgcc agttcaaagc cctctgagat tgcggacctg gctattgtgg
     3481 ggcagggaag attttggacg gtaaatttgc cggatccctt gttcaaatat gtgcgctggt
     3541 gtggcgatat cgatatctat gaaaaccctt cgcatcgact catcggtgtg atttcagaga
     3601 cgggacggga agggcatgtg accgaaaagg aagcgcggat tcgtgccagc aaagtcatgc
     3661 aggacgtcat ctccaagaaa atcgcccgtc tacttactca atccatgttc agtgccgaac
     3721 cggattcgtc acagagtcag agcgttccca acgcctgttc tcgctaacac acagtcttct
     3781 tgatctcttt aaagaatttt ttgttgcggt agatgaattt agctggtcag gccgattaaa
     3841 ggctaacagg acaagtcttg ctccaggcct tggaggagtc tttcccattc ttgggtcttt
     3901 ccgatgtgcc tttgctgacg atccctcaac aattcattgg tgaaatccgc caaggctttg
     3961 agatcattct gtgattgttt caccttttca gcagcatggg aaagatattg acagacaccc
     4021 ttggatgccg gtggctgacc gcccgctcgt tcaaagaggg caaaagcacg atagccctgt
     4081 tcttggcctc taaagagggt ttcttccgcc tgatacagca agcgagtctg ttggtgttct
     4141 tccttttgag cgagaatttt tgccatgagc gcggcctttc caatggagag ggcggcgcct
     4201 tcaggatcac catttgcaat agcatattca gcttgctgct ccaggcgatc catcccccca
     4261 atttctccag gcacttgcgc caggccgttt cccgcatacg ggatcatgag tacgataagg
     4321 agcacccaaa tctctggggg ctgagggaga accggtttca taacgtcagc cgataagctt
     4381 caagatgagc agagctactg aatctgcgaa tccatttcga tttgtttata ggtccaactc
     4441 gtggcctccc gcagttcggc catttctttc gaattgtcat gaatcaccca aattttattt
     4501 tccagctcag aaatgggggc aacagctttt gcatttcgca gtttgcctaa actccacacg
     4561 acctcagtca taacgggaaa atcgatgtcc gaagatgtct tgatgtgtga aagattctca
     4621 acaacatagg caagcatggg tggggtgatg gtgggatctc cggtcagcga ggcaatttcc
     4681 cctaaccctt tggctgcggc cgctcgaatc gccgagtccg tctgggtgtg aaaaatttct
     4741 gtcaggatgg gaatagcttc gcttcccgcg ctggacatgg ccacgatagc ttcttccgct
     4801 atttcaggat cttggatgag ttctttcagt aagggaatgt attgttggga ttccacctga
     4861 ggcattaagg aaaagattct aatctttcgt ttatttggtg tctgagggtt attgaggagc
     4921 ttgaaaagtc tggtattatg tccccattcc gcagcgagca taacggggaa atcgaattct
     4981 cgaatttgct tggctaattc ttgaatggca tagggaccgg gctgtggtgc attcgcggcc
     5041 tgggcggctt ggccggaatc ttcaaacttg gttcgcgctt ctttgtacca acccaggtct
     5101 cgtccatcca actgatagga aaaaagacag gccggcccct tccaaagacg ggagggggac
     5161 ccgatttggt cggcatcgcc gccggcccga gtggttcctg accatgtttt ttgttcctcg
     5221 catttgacgt ttaccctcac atcgtatgga acatcccgac tgatcacgac ggtgtacccc
     5281 aattcttcca atcgctgtcg tacaacatct tctatgacgg tcccatctac tttcccatgc
     5341 tctgtcaacg ccaggacatg aatcagaatg gtttgaatgg cgtcgagttt cgctttttgg
     5401 tctgcgtcga gatacgtccg cctggccaag ccctctgagc cgaaaagccc aacgagcaaa
     5461 actatggtaa tgaatccaca tgttgcccat gccctcatgc ctgacctccc actgtacaat
     5521 gtgaagtgtt aactgcgtat gatcgcaaat tcccaaccat catgaccctc atggtgtatg
     5581 acttggttga caggaaatcg tcaaaaattt ctcagaattg tcgagcctat gattaataag
     5641 tttgcaacat cgcagcatcc tgtaacgcta tggctttata ggcccaacta gtggcctctc
     5701 gcaattcggc catttctttc gaattgtcat gaatcaacca cactttatcc tgtagttccg
     5761 aaatcggctt gatcgagggg ccccatcgca attttccaat tgcccagacc acttcggtga
     5821 gcaaggggaa attaatatct tctgaggttt ttatatgagg taacacttcg gaaacgtaat
     5881 gcgccaaggg gggaattgtg cggggatctc ccgacttgga agcaataatc cctaaggctt
     5941 tcgccgcttc ggcccgcaga ttcgattgcc gactggtttg gaataaatcg atgagtaatg
     6001 gaatagattc gattccggca cccgagagcg cattgatggc ttcttgctgt aagtccgtgg
     6061 actcgaggag tttggtcagt tggggaagcg cttcttccgc atgggcgtca ctgaggaccg
     6121 aaaggatttt taccttccgc aatttgggtg tgttgggatt atccaacaat ttgagcaggc
     6181 gatcaatttg gccccactcg gcagagagca ggacagggaa gtcaaattcc ataagccttt
     6241 ggttgagatg ctccatcgca tatttcccgg catcgtcaac ttgggcctct tgggcggcct
     6301 gcctggcatc agcaaagttc gtgcggacct cttttttcca ttgaaaatcg ttctgttgga
     6361 ggaaataggt caacagacag gctggcccct tccagagccg atcaggcgca tcggccaggt
     6421 ccacgtcccc gccgctggga gaggtgccgg tcaatgtttt ttgttcttca cattttactt
     6481 tcacttccac atcgtacggc tgcgaccttt cggtggtgac tgtatacccc atttcttcaa
     6541 aacgcttctt gactgtggca gtcaagggct gggcatcata cctccctttt tctgttaagg
     6601 cgaggacctc gaccaacaca atctgagcgg tttctaattt ggttttttgc tcagtggtcg
     6661 gagaaatctg ccgggcgtcg gattgagtgc catcaaccca caacattgcg aaaatcaaga
     6721 aaacaggcca ggctttcata ccttacctcc ttggcttcta atttttgacc attgtggatg
     6781 gcaacgtagg cgaaataggg acgaaacaaa tgtttctaaa cgaattttcc aggatttttt
     6841 aatatacccc tcacattaag caaggggcaa cccaatgtcg tcgtgaaaac ttttgaccag
     6901 gttgctttgg caagctgctg atcgtcctgc cacatgcaga ggctttattg aaaaagttag
     6961 ttactcgata tcaatattcg ttacagaaaa agtcctcaat ggttttgatt gggaatacgc
     7021 agactgaagg attttattaa tatttcctgg ggataagttg aacccctatc agtctccggc
     7081 tatactcggc gcatgtcaca gtcaccaaag ttggcatttt taggagcagg taatatgtgt
     7141 gaggcattgg tgaccggcat tttgaaagcc aaccttgcgt ctcctgccaa tatttctgtg
     7201 accgatattt cctcaatccg attagaacat ttccaacaga catttcaagt ccaggtggga
     7261 tctcataacg ctgatgaagt gaagtcagcc gatatcattg ttctctgtgt gaagcctcag
     7321 gttatggata tcgtgctttc ggagatcaaa gatcagcttt ctccgaagca tttagtgatt
     7381 tctgtcgcag caggctatcc gttggctcgc atacaacagc atatcgggga aaatgtttct
     7441 ctaattcgtg cgatgcccaa taccccggca gtcattcagg aaggcgtaac ggctttagcc
     7501 gggagttcag gtcttccctc ccagcatctt ctgctggctc aatccatatt tgaatcggtg
     7561 ggcagagtgg tcacggtgga agaatcctta atggatgccg ttacgggttt gagcggaagc
     7621 ggtcccgcgt atatctatct ggtgattgaa gcactcaccg atggtggagt gcggatgggc
     7681 attccacgga ctgttgccac ggtgcttgcg gcacaaacag ttttaggggc ggccaagatg
     7741 gtactcgaaa gtggggaaca tcctgcttta ctcaaagatc gggtgacttc accaggtggg
     7801 acgacaattg ccggactcca acaattggaa atcgggcgga tacgggcgac cttaatgaat
     7861 gccgtcgaag ctgctacggc tcgttctcat gagttgggcc aataatgaac tcggtcgcac
     7921 tgacgtgaga tgcacacatg gcataagatt ttattgaaag gagtacacgg tgcagtattg
     7981 ggtcaaagtc atttttgttg ataataaaga attagaattc aaggacgcga ttcgccatac
     8041 catcagcgat gacatggaaa ttcttgaaat tgatactccc aaggaagtca ccatcattcc
     8101 cttgaagcaa attaagtatt tttcttgcga tgccggagtc tttgcaaaaa aataaagtag
     8161 aggcagtgaa ttggaccagg ggtctcttaa tattccgatt gaattatccg aaaggattta
     8221 ggaggttctt gttcgttata tggtcaaatt tcctattttc ttatttataa tggaaagacc
     8281 ctaaacctaa ccccttacat aatgcttgcc aacagaaagg tatttgattt cgtctcttat
     8341 aaagggaaat aagaaaaact cttcttgcct aattcatctc tttatgccac catcatatta
     8401 ttcctgtaat ccgacgtgga tcttctttca taaaaacccc aattgaaatt tggaatgaca
     8461 attcgttgaa gacgcggaag gaataatcat gatcatggaa acgatcaaac aggttggtaa
     8521 acaaatcagt ctggtcctgg tcttattgct cgggatgttg atcctttggg agcttcccca
     8581 ccatgatcga aattacctct cccatcagga gcggaccgcc caattaggaa cgaagctgcc
     8641 gtcgtatgag gtaaagacct ttttgcgcca tattgagact aggcttccct cttatcggga
     8701 agaatttcag caggctgaaa aaaaatcagg gatttcatgg atgttattag cagccatggc
     8761 ctatcaagaa tctcaatgga accacaaggc agttagtccc acaggggtgc gaggaatcat
     8821 gatgcttaca cggtccactg cctcggatct gggaatcaaa aatcggcttg acccttcaaa
     8881 aagtattgcc ggtggagccc gttatctatc ctatttgcaa aaacgggttc ctgacaatat
     8941 tcgaatgcca gatcgcatgt tttttgccct tgccgcctac aatgtcggca tgggacacat
     9001 taacgatgct cgattgcttg ctaagcgcct aggtaaaaac tctgatcagt gggaggacct
     9061 taaaagcatt cttcccctcc tggctcacaa agaatattac caggatctgc cacatcgtta
     9121 tgcccgtgga tgggagcctg tcaaatatgt gaagcgtatt cgagcctatc ggaacatcct
     9181 tcagcaggtt atgaagcgtg aagggaaaat gtctcaagtg gatatttaat tcgttcctct
     9241 gttgtatttc agttgatctt cagcctctct cggaaaacat atttctggct ttattccctt
     9301 cattttagtc ctttcgcatt cctcgacctt ccggatttct tccctcttca aaatttccag
     9361 gtgtgagatt aatgcgcgtg ggcctgcccc caaaaacagg gaccatcatt tgtgagatct
     9421 ttggagatga aatgatctaa tattcgcgga aaaataaatc tgatctctag ggcatcttag
     9481 tcccaaggga ttcatctggc ttagcgcgtt cgatggatag tgcagtatca tttggtaaaa
     9541 ttttggcttt aatcctatct ccaaatttaa aggattccga
//
LOCUS       DAORAQ010000097         9525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1147_length_9525_cov_70.467265, whole genome shotgun sequence.
ACCESSION   DAORAQ010000097 DAORAQ010000000
VERSION     DAORAQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1147_length_9525_cov_70.467265"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKM72_09285"
     CDS             complement(<1..689)
                     /locus_tag="PKM72_09285"
                     /inference="COORDINATES: protein motif:HMM:NF039283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5911 domain-containing protein"
                     /protein_id="HNP61020.1"
                     /translation="MSYKNIGDYGIIGDLHTIALVGIDGSIDWCCLPRFDSPSIFGAI
                     LDHKIGGYFKIAPVTNGNTRQMYLPETNILLTRFLQHDGVGELTDFMPIESDEAGYRP
                     RRHQIIRMLSVVRGTVTFRLECTPAFNFGRNPHDIETRPKGIIFQSGDQSVGLVSPIP
                     LHTRENFAYQEFTLHQGESLTFFLEYLEVKNGDQLLSTPESGDEAFRNTSAFWQRWLA
                     QCQYDGRWREVV"
     gene            1419..2528
                     /locus_tag="PKM72_09290"
     CDS             1419..2528
                     /locus_tag="PKM72_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNP61021.1"
                     /translation="MTPKTSPSSTGLDLSRVSFTLRNHLPFTAELEQCIPLAGDASNR
                     RYYRLHLSMAPVTSLILMQLADPEGFKVSEEAVSGAGGEITELPFTNILKHLQKNGVQ
                     VPVLYFYDESSGLLYLEDFGDVTLAQAWQKSSPGTGEDLYCQAIDQLVHLHLQASRAS
                     TVPCVAFTRSFDVALYLWEFDHFLEYGIVTRDRPMCVDDYVPVREEFKEIAEWLTAQP
                     KVFTHRDYHSRNLMVDGERLGVIDFQDALMGPVTYDLASLLRDSYISLDEELIDRLIA
                     RYIEGMRKNLSLSQQSAMLLHDPVAFRRLFDFTSIQRNLKAAGRFVYIDRVKGNSSFL
                     ASIPQTLKNVRANLDKYPQLHRLLTHLSPYIPEWR"
     gene            2542..>2939
                     /locus_tag="PKM72_09295"
     CDS             2542..>2939
                     /locus_tag="PKM72_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNP61022.1"
                     /translation="MKAMILAAGLGTRLRPLTNTIPKPLLPVGGTPLIIWNLLMLRAC
                     GICDVIINLHYLGHLIEEVIGDGSGWDMQVRYSREPTLLGTGGGLKAAEWFFGQHPFL
                     VINGDTLIDLDVKTLVEFHQTHGGLATLVLR"
     assembly_gap    2940..3039
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3372..5318
                     /locus_tag="PKM72_09300"
     CDS             3372..5318
                     /locus_tag="PKM72_09300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNP61023.1"
                     /translation="MAIFNQLHHLLEKSLRDFQAKSSDLSDTQEQELTQALHRLTGRL
                     TEILSTNSDPYPTAELLSGCSQTVSLRAPTLLPEPPQIHFVTSCDGVVLMANEVVGDV
                     LGMDLGEIGQVSIADYVAHEEWEILRNHLCALDVPPKLLNSMLTILPAGKPSRTIPCV
                     VTPMLDQSQKVTAWHWGLTLEAEHSSSQPFSQLMQSLESQLYAGQSLDVCLTKICDAL
                     VQAFGFPFVWVATVRKGSVPQLRAHAIAEAFDWESQGALWWSEVSQQAEFFQACEASE
                     MLLLSSCLSPKGGMVWFPPGLQLHQIWSIPLSFQENCSGMLVVGSQGPQPVDPTVLGW
                     LQALGYEIERLMAHGKQLDQWRLQSVALGSVFDPICVTDTQGRLEWVNEAYAALHGSS
                     TQSLLGFPLSSFPHAELQVRRSLSDPSTQEFNFVKTEVLQTGTNGQSLVFEQVVTPLL
                     NEQEDLTHFVVIWHDVTARKTQELMMKHQAYHDPLTDLPNRIMFEDRLEQALAQARRN
                     GTLLALFFLDLDNFKTINDYHGHQIGDRVLRIVAERLTTCVRSTDTVARLCGDEFTVI
                     LQGLEHVQDIRQVALKILECLIPPIRLGKEKIPIQISIGISVYPKDSTDPHRLVEIAD
                     QAMYRAKECGGQCWRFATSEWNDE"
     gene            5899..6645
                     /locus_tag="PKM72_09305"
     CDS             5899..6645
                     /locus_tag="PKM72_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNP61024.1"
                     /translation="MTVHSSTRRIYVWGIVILMMLLATGCGHRSSQGVHKYGKKTSAP
                     TAVAAQGDFTTGPSDQALNNGSSAEEGYFPEGTGTFSDLLAKDSLIREDNTFGAGLTP
                     SDSPNDYWENRTRAEQLTAQSGLRDVHFEFDSFQLNEQAKVTLVANADWLKAHPHAEI
                     TIEGHCDDRGTASYNHVLGEKRAIRTKTYLSSLGVPAERLHVMSFGKESPACWDSTEP
                     CYQKNRRAHIVLDISVASTPMPYVADRRDW"
     gene            6700..8130
                     /gene="ybgF"
                     /locus_tag="PKM72_09310"
     CDS             6700..8130
                     /gene="ybgF"
                     /locus_tag="PKM72_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNP61025.1"
                     /translation="MLSPHSFSYRWSVRLVIVALNVILAGCMAQQADVVRIKRELDAK
                     IGQLDKSKTSLQQAVSEANTALEKANTLIAKQRVEIQELLHARAEVMDQVATLKETDL
                     SQVRGGVESNQNQVSELTKKMARYESDMKDVRTQLQQSEPLVHQLRDRLAGEEQLLTE
                     QGGKLGEFRTSLVDYQQVLASLRQQVSQQEQQVGELRRQIELKAQQHDAQAQQVQANF
                     EEVRRSIQSVVGTLEKVSVTFGGRLDEHEQKLSSVAGQHNSSLSSNAQMPESRDPSAR
                     SAVTRDLFPQRTPPSSRSDAMDGLIATKERSHPLVTGSVAAYSPSSQPSRTLPALGGP
                     SGTGSSNNVDMQNGQVIYDRAMSFLRQGKFSEASKGFSTFLRTFADSPLASNAQYWLG
                     ECYYGERRFQEAIDEFERVFAFYPSSNKVPASLLKIAYSHIELRELPMARSVFQQLVR
                     THPQSPEADKAYGRLQEVNAFLDNPS"
     gene            8513..>9525
                     /locus_tag="PKM72_09315"
     CDS             8513..>9525
                     /locus_tag="PKM72_09315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNP61026.1"
                     /translation="MLLHVCFGASDGYLPGFYEKIEWGLGKNIILQGFYAVDVFFVLS
                     GFILTYVHRHEFEGRLTLHGVGSFLSLRLARIYPMHLVVVVVLIAAAFLGFWDQKAIS
                     YGDVFRNGTLTNIWVNPSLNTPAWSVSAEWIAYLCYPIIIKLLIPIRRRDFQLLIIFF
                     LVTVYPMCIIFFQWQWEWHYGWVAVARVLNGFILGCMMFCVQKHFDILNDPLRASRWC
                     LVFLLVFMAFLILGLPIVFLYPLIPFIIVTLANARMGIAQIFENKIVVFLGTISFGIY
                     MVHYPVLEIVRSEFNDYYSGVNSELNQPLLWMHLCGILGFVIAVASLCYFWIEKPTRD
                     YLKR"
BASE COUNT         2256 a         2077 c         2377 g         2715 t
ORIGIN      
        1 accacttccc gccatcgccc atcgtattgg cattgtgcca gccagcgctg ccagaaggcg
       61 gacgtattgc gaaaagcttc gtcgccggat tcgggggtgg ataagagttg gtcgccgttt
      121 ttaacttcca agtattccaa aaaaaatgtg agactttcac cctgatggag tgtgaattcc
      181 tgatatgcga agttttctcg cgtatgaagg ggaatgggac tgaccaatcc cacggattga
      241 tctccggatt gaaagatgat gccttttggt cgggtctcga tgtcatgagg attcctgcca
      301 aaattaaatg cgggcgtgca ttcaagtcga aaggtgacgg ttccacggac aactgagagc
      361 attcggataa tttggtggcg gcgaggccgg tatccggctt cgtccgattc gatgggcatg
      421 aagtcagtga gttcgccgac cccatcatgt tgcagaaacc gggtcagcag aatattggtt
      481 tccggcaggt acatttgtcg ggtattgcca ttggtgacgg gggcaatctt gaaatatcct
      541 ccaattttat gatcaagaat ggctccaaat atgctggggg agtcaaatcg tggtaggcaa
      601 caccaatcaa tggatccatc gataccgaca agagcgatag tatgcaggtc cccgataatc
      661 ccgtaatcgc cgatgttttt atagctcacg gatggttctc atcagaagaa atatggctga
      721 atcaagaaag acgaaaacta atatacccaa aatagaacaa taagcactac atatagcgat
      781 aaaataatta ttaaggctca atatctagac ttagtttaac ccaaggacga aatttaaaat
      841 tatgcgcagc gcatctgcag agtatttctt cccgtgatca aaatccgaaa tggcgttaag
      901 gtttagggga ggggtaaaca tgaaaaatca agcagtaacc agtgttacac catgatatca
      961 atgagtcgtg ttgagagatc tatctggagt ggtggttctt cggagtgttt cccaaagttt
     1021 tgttgagggt tttttctgtt gcggctaaaa aaatttcctt tctgacccac atctccatcc
     1081 tccactgctg aggaggactg aattggagga agaggtggcg gggatgaatt ggtgatttcc
     1141 atagtgcacc tttacatttg tgggtgatta tggtggcttg tgaggcgttt ccctctctta
     1201 ttactacttg tcgtcaaaaa tttctcaatc ttaaatgcaa ggggagaaag atgtcgtcag
     1261 ttcgtaagca gggcatcctt tggtcttgcc tgtgcttctc caagttctcc gagtctccaa
     1321 tgcgttcggg cctaggcaga ggaattccat tgcaaaatga ggttgtcttc tttcctaatt
     1381 aatgacaaaa agttggtatg gccatataat caaatgaaat gacgcccaaa acttctccgt
     1441 cttcgacggg tcttgatctc tcgcgtgtgt cctttaccct ccgaaaccat cttccgttca
     1501 ctgccgaact tgagcaatgc ataccgttgg cgggcgatgc ttcgaaccgt cgttattacc
     1561 gtttgcactt gtccatggct ccagttactt ctctgattct gatgcagtta gccgacccgg
     1621 aaggctttaa agtctctgag gaggctgtaa gtggggccgg gggagaaatc accgaattgc
     1681 cgttcacgaa tattctgaaa catctgcaaa agaatggcgt tcaggttccc gtgttatatt
     1741 tttacgatga atcctctggc ctgctctatc ttgaggattt tggtgacgtg accctggccc
     1801 aagcctggca gaagtcttct cccggcacgg gggaggatct atactgccag gccattgatc
     1861 aactcgttca ccttcatctc caggcctcac gtgcttctac tgttccctgt gtggccttta
     1921 cacgatcttt tgatgtggcc ctgtatctct gggagtttga ccattttttg gaatatggca
     1981 ttgtgacgag agatcggccc atgtgtgtcg acgattatgt gcccgttcga gaagaattca
     2041 aggagattgc tgaatggttg accgcgcaac cgaaggtatt tacccatcgt gattaccatt
     2101 cccgtaactt gatggtggat ggggaacgtt tgggcgtcat tgattttcaa gatgccttga
     2161 tgggccctgt cacatatgat cttgcctccc tgctgaggga ttcctacatc tctctcgatg
     2221 aggagctaat cgatcgtctt attgctcgtt acatagaagg aatgcggaag aatttatcat
     2281 tgtcgcaaca atcggctatg ctattacatg atcctgtggc gtttcgtcgg ctatttgatt
     2341 ttacgagtat tcaacgcaat ctgaaggctg ccgggcggtt cgtatacatt gatcgtgtga
     2401 aagggaattc aagctttttg gcctcaattc ctcagacatt aaagaatgtt cgtgctaatt
     2461 tagataaata tccccaactc caccgactgc tcacccatct ctctccgtat attcctgaat
     2521 ggcgctaact tcttgcttat tatgaaggcc atgattttgg ccgcgggtct gggtactcga
     2581 cttcggccgt tgaccaacac gattcccaaa cccttattgc cggttggagg gacgccgctc
     2641 attatttgga atttgttgat gttgcgagca tgtggaattt gtgacgtcat catcaatctc
     2701 cattatttgg ggcatttgat cgaagaggtg attggtgatg gatcaggttg ggatatgcag
     2761 gtgaggtatt cccgtgaacc aaccctgtta ggaacgggtg ggggattaaa agcggccgag
     2821 tggttctttg ggcaacaccc gtttcttgtt atcaatggtg atacgttgat cgatttggat
     2881 gtgaagacat tggtcgagtt ccatcagact catggtggtc tggccacctt ggtccttcgn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aaaattattt ggatttcttc
     3061 atgacggcta ctggtccgat attggaacga tggcccgtta tgctcaggcg caagccgatg
     3121 tggaggccgg agtgatagct cttaagacct aaggtggtcc ttctgatgat aggctgccca
     3181 gcattttcct ccatggattg agttgtctag gatatgctgc gaataggttg cttaggaatt
     3241 ggcgccaaaa atcgcacccc cttttgacgg gttgacgtcc agagaagctt gccaaaagat
     3301 atcgatatcg ggtttattat ctaaatactc cctgtgtttt tcccattcct tgcggcaagg
     3361 tgaaggtaat cgtggccatt ttcaatcaac tccatcatct actagaaaag tccttgcggg
     3421 attttcaagc gaaatcgagt gatctttccg atactcaaga acaggaactt acccaagccc
     3481 ttcaccgatt gacgggtcga cttaccgaaa tactttcgac aaattccgac ccatatccta
     3541 cagccgaatt gctttcgggt tgcagtcaga ccgtttcgct gagggctccc actcttctac
     3601 cggaaccacc tcagatccat ttcgtgactt cgtgtgatgg tgtggtcctc atggccaatg
     3661 aagtggtggg tgatgtcctg ggaatggatc tgggtgaaat agggcaggtt tccattgccg
     3721 attatgttgc tcatgaggaa tgggaaattc ttcgaaacca tctttgtgcg cttgacgttc
     3781 ctcctaaact cttgaatagc atgctgacga ttctcccagc ggggaaacca tcgcgaacga
     3841 tcccatgtgt ggtaacccct atgcttgatc agtcgcagaa ggtgactgcg tggcactggg
     3901 gattgaccct ggaagcggag cactcttcct ctcagccttt ttcccaattg atgcagagtc
     3961 tcgaatcgca attgtatgcc ggtcagagtc tggatgtgtg tctgacgaag atttgtgatg
     4021 cccttgtgca agcctttggt tttccctttg tgtgggtggc cactgtgcga aagggtagtg
     4081 tgccccagtt acgcgcgcat gcgatagcag aggcttttga ttgggagtct cagggagctc
     4141 tgtggtggag tgaggtttcg cagcaagctg agttctttca agcttgtgag gcatctgaaa
     4201 tgttacttct ttcttcttgt ctttccccca aaggtggcat ggtctggttt ccgccgggat
     4261 tgcagttaca ccaaatctgg agtattccct tgtcttttca agagaattgt tccggtatgc
     4321 tggtggtggg ttctcaaggc ccacagccgg ttgatccgac ggtacttgga tggttgcaag
     4381 cattaggata tgaaatcgaa cggttgatgg cgcatggaaa gcagctagat caatggcgat
     4441 tgcaaagtgt ggctttgggc tcagtatttg atccaatatg tgtgacagac acccagggac
     4501 gtttggagtg ggtgaatgaa gcctatgccg ctttgcatgg ctcctcgact caatcactgt
     4561 tgggttttcc gttgagctcg tttcctcatg cggaactgca agtcaggagg tctctgtcag
     4621 atccctccac ccaagaattc aatttcgtga agaccgaggt tttgcaaacg ggtaccaatg
     4681 gtcaatctct ggtttttgag caagtggtga ctcctttgct taatgaacag gaagacctca
     4741 cgcattttgt ggtgatctgg catgatgtga ctgcccggaa gacacaagaa ttgatgatga
     4801 agcatcaggc ctatcacgat ccgttaaccg atctcccgaa tcgaattatg tttgaagacc
     4861 gcctggagca ggcgttggcg caagccaggc ggaatggaac tttactggca ttattctttt
     4921 tggatctcga taatttcaaa accatcaatg attatcatgg tcatcaaatt ggagatcgcg
     4981 tcttacgcat cgtggcggag cgtctgacaa cttgtgtgcg atcgaccgat actgttgcgc
     5041 gtttgtgcgg ggatgaattt accgtgattc ttcaagggct tgaacatgtt caggatattc
     5101 gtcaggtagc attgaaaatt ctagaatgcc ttattccacc cattcgatta ggaaaagaaa
     5161 aaattcctat tcaaatcagt attggaattt cggtttatcc caaggattcc acggatcccc
     5221 atcgattggt agaaattgct gatcaggcga tgtatcgggc taaagaatgt ggtgggcaat
     5281 gctggaggtt tgccacgtct gaatggaacg atgaatagtc cttgccaccc taattctctc
     5341 ctcctgcatt catcctgagg ccgtccattc tcccaaaatg gggattccat ttatccttct
     5401 cactaactga ctgtgctcgg ccggcttttt caaaaattca ttgatcccgc gcatactttt
     5461 cccgcctaag aatctcccat gtttttgtgt ccttaaaacg cgttgagaaa aagccacgat
     5521 tgttgattaa atgctgcttc agttatatca ctcacctctt cttgttgtgg tggttctttc
     5581 ccaactcctc cattaatacc tccctgaatc ctgttcatat tgtcagaagg gcattgggga
     5641 cggttcttgg cggaactctg gggtgaatcc cctaactctg cagtacatct atggaatttg
     5701 aggtaattgg tgtgggcgct tttcttataa ctgtttgaaa gcgaggggga ctttgtgatc
     5761 gaaacggctc gacgaggagt gcctatcgta tcggcatagt cccttggcct tgtttttgct
     5821 aattcttatt gtagttgaag aatttcaaga ggcatttcgt ggtttgttcg ttgtgctgtt
     5881 gaacaaggag ttcgaaatat gactgttcat tcatcgaccc gcagaatcta tgtttgggga
     5941 atagtgattc tgatgatgtt gctggccaca gggtgtggac atcggtcttc acagggagtt
     6001 cataaatatg gcaaaaaaac tagcgcccca actgcagtgg ccgcacaggg agattttaca
     6061 acgggacctt cagatcaagc actaaacaat gggtcatccg ccgaggaggg gtatttcccg
     6121 gaagggacag ggactttctc cgatttgctt gcgaaagatt ccttgattcg ggaggataat
     6181 acatttgggg ctggtctcac gccttctgac tctcccaatg attattggga gaatcggact
     6241 cgtgcggaac aattgaccgc acaaagcggt ttgcgggatg tgcattttga atttgatagc
     6301 ttccaactta atgaacaagc caaggtgaca ttagtcgcca acgcggattg gctgaaagcg
     6361 catccgcatg ccgagattac cattgaagga cattgcgatg atcgggggac ggcttcgtat
     6421 aaccacgtct taggtgaaaa gcgtgcgatt cgcaccaaaa cctatctctc aagtttggga
     6481 gtccctgctg aacgactgca tgtgatgtca ttcggtaagg agagcccggc ctgttgggat
     6541 tccaccgaac cgtgttacca gaagaatcgg cgcgctcata tcgtcctaga tatcagtgta
     6601 gcctcgaccc caatgcccta cgtcgccgac cgccgggatt ggtagaggtc ctttgcgggg
     6661 catggaccgt ttatggtttc tgagaggata aggaagacta tgctaagccc tcattcgttt
     6721 tcgtatcgtt ggagtgttcg acttgtcatt gtggccttga acgtgatctt ggctgggtgt
     6781 atggctcagc aagcggatgt ggttcgaatc aaacgggaat tggacgccaa aatcggccag
     6841 ctggataaga gtaaaacatc actccagcaa gccgtgagtg aggcgaatac ggcattggaa
     6901 aaagcgaata cccttatcgc caaacagcgg gttgagattc aagagttact gcatgctcgt
     6961 gccgaagtga tggatcaggt tgccacctta aaggaaacgg atctctctca ggttcggggg
     7021 ggggttgaaa gcaatcagaa tcaggttagc gaattaacca aaaaaatggc gcggtatgag
     7081 tcggatatga aggacgtccg aacccaactt caacaatctg agcctctggt gcatcaactt
     7141 cgagatcgtt tggcagggga agagcaactt ctgacggagc aaggaggtaa gttaggcgaa
     7201 ttccggactt cgttagtgga ttaccaacag gtgctcgctt ctcttcgcca acaagtttcc
     7261 caacaggaac agcaggtagg ggaattacga agacagatcg aactgaaggc gcaacaacat
     7321 gatgctcagg cgcagcaggt ccaggcgaat tttgaagagg ttcggcgaag tatacagtcg
     7381 gtggtaggga cgttagaaaa agtcagtgtc acattcgggg ggcgattgga tgaacatgaa
     7441 caaaaacttt ccagtgtggc tggtcaacac aatagttccc tttcctccaa tgctcaaatg
     7501 cctgaaagcc gagatccgtc agcgcggtca gctgttacca gagatttatt tcctcaacgc
     7561 actccaccat cgtcgcggtc agatgcgatg gatggcttaa ttgccaccaa ggaacgttct
     7621 catcctcttg tgaccgggtc ggttgccgca tattcccctt catctcaacc atcgaggaca
     7681 ttgccagctc tgggagggcc ttcaggaacg ggttcttcca ataatgtcga tatgcaaaac
     7741 ggccaggtga tttatgaccg ggcgatgtca tttcttcgtc agggtaaatt tagcgaagct
     7801 tcaaagggat tttcgacatt tttgcggact tttgcagact ctccgttagc ctcaaatgct
     7861 caatattggt tgggggaatg ttattacggg gaaagacggt ttcaagaagc gattgatgaa
     7921 tttgaacgag tctttgcctt ttacccttcc agcaataaag tcccggcatc cctacttaaa
     7981 atcgcctatt cgcatatcga attacgcgag ctgccaatgg cccggtcggt ttttcaacaa
     8041 cttgtacgga cccaccccca aagcccagaa gccgacaaag cgtacggtcg tctccaagaa
     8101 gtgaatgcct tcctcgataa cccttcctaa atttccaatt acccctgcgt cgttttttct
     8161 catcttgtct cacatagacc acaccggaat tttttctcct ggagtgaatg ttttgtctgt
     8221 tcagggaacg aggaacttgc cagctcgtcc ctgtcatgat tccagagaaa agtgcctggt
     8281 tttatagtca ttcttaaatg aatcctctcc tcataatccg tgaatagtag aagtaggtcc
     8341 gaagtctgct tcatgcaaga ttttcatagg accctattca agatgttctc ttcacagacc
     8401 gaagcttcgc ttacggtggt ttatcaactc tgatcacaaa actatgctat ctcggaacag
     8461 ggaaaatgtt ccaaccttga caggcctcag gggggttgca gcgatatggg tcgtgcttct
     8521 gcacgtgtgt tttggggcgt cagatgggta cttgccggga ttctatgaaa agattgaatg
     8581 ggggttagga aaaaatatta ttctccaagg attctatgcg gttgatgtct tttttgtcct
     8641 tagcggattc attctgacgt atgtgcatcg ccatgaattt gaaggtcgat tgactttgca
     8701 tggagtcggg agttttcttt cgcttcgatt ggcgagaatt tatccaatgc atctggttgt
     8761 cgtcgttgta ctcatcgcag ctgctttcct tggattttgg gatcaaaagg ccatctcgta
     8821 tggagatgtc tttcgtaatg gcacactcac aaatatttgg gtaaatcctt cgttgaatac
     8881 tcctgcctgg tccgtgagtg ccgaatggat agcctatctc tgttatccaa ttattataaa
     8941 acttctgatt ccgattcgac gaagagactt tcaactcctg attattttct tcctggtcac
     9001 cgtgtacccg atgtgcataa ttttcttcca atggcaatgg gaatggcatt atggttgggt
     9061 ggctgtggct cgggtgttga atggatttat attagggtgc atgatgtttt gcgttcagaa
     9121 gcattttgac atactcaacg atcctttgcg ggcatctcgt tggtgtctgg tttttttatt
     9181 ggttttcatg gcgttcctga tattgggact tccaattgtg tttctgtatc cccttattcc
     9241 atttattatt gtaaccttgg caaatgcacg aatgggaatt gctcaaatat tcgagaataa
     9301 gatagttgta tttttaggaa ccatctcatt tgggatttac atggttcact acccggtatt
     9361 agaaattgtt cgatccgaat ttaatgatta ctattcagga gtgaattcag aactcaatca
     9421 acctctgttg tggatgcatt tatgtggaat tcttggtttt gtgattgcgg ttgcctcctt
     9481 gtgctatttc tggattgaaa aacctaccag ggattatctc aagcg
//
LOCUS       DAORAQ010000098         9473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1156_length_9473_cov_68.991824, whole genome shotgun sequence.
ACCESSION   DAORAQ010000098 DAORAQ010000000
VERSION     DAORAQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_9473_cov_68.991824"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..446
                     /locus_tag="PKM72_09320"
     CDS             27..446
                     /locus_tag="PKM72_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNP61027.1"
                     /translation="MKRLFFSLTIALLFSGWWNLPIVTAEPYLLTVQQLKAGLEKSSQ
                     SKQKGFVLIDVRSPDEHMSGFIPGTDFNIDFREMQGRHRELNAKLDQHIVVYCQSGHR
                     SNIAAETLMGLGYQNVYNVEGSMNAWTEAGYSIEHPK"
     gene            complement(473..1348)
                     /locus_tag="PKM72_09325"
     CDS             complement(473..1348)
                     /locus_tag="PKM72_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNP61028.1"
                     /translation="MRKGKPAEDGIPSSSLMGHSKDEASQESATGKLQAARLQLMAYA
                     KDLKQMLEQEGQKTQQLKKAHAQMLAYARDLKLSLEAEQRKNSELEQAYADTILRLAR
                     ASRYKDEETGAHIERLSHYSHSLALAIGWDQPRARCLFAVAPMHDVGKIGVPDAVLGK
                     QGPLTEEEWQSIKQHPLLGASLLDGSTSPLLEMAKEVALTHHERWDGSGYPRGLKGTQ
                     IPLTGRIVMLCDLYDALRSRRPYKAAFTHAKTCDIILNGNDRTKPTHFDPLLLEAFRE
                     MHQNFNGIYSTVAEN"
     gene            complement(1345..1722)
                     /locus_tag="PKM72_09330"
     CDS             complement(1345..1722)
                     /locus_tag="PKM72_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61029.1"
                     /translation="MVGPVKTIIIADDEEDLRLLVQVTLEHPSYRILTAVDGCAAMDA
                     VCTHIPDLLILDWMMPGLDGCEVVRNIRLRSDTARIPIVMLTAKDGIDAREQMASLNL
                     AGYLVKPFSPLELIQKVREVLAS"
     gene            complement(1757..2656)
                     /locus_tag="PKM72_09335"
     CDS             complement(1757..2656)
                     /locus_tag="PKM72_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNP61030.1"
                     /translation="MKNFQSGERVHLIDKKERPYAVTLKAGEIFQHSGDRIAHDDIIG
                     KPDGTVVQFSNGKLMVAVHPTLAEYTLKMPRGAQVIYPKDLAVIPMWADIYPGARVFE
                     AGVGSGALTMALLRAVGDRGCVVSYEMREDFAATATKNIERFMGKVPNHFLQIRDAYE
                     GIEIGEGTSSWMFDRVVLDLPEPWRVVPHAARALRSGGLYLSYVPTVPQVMQTVRALE
                     QSRVFTMVQNFETLLRTWNIKGRSVRPDHRMVAHSGFITVARKVEKNIWQTEQSNTIE
                     PESQSEDQLIKEDEYAGNGLDTP"
     gene            3016..3705
                     /locus_tag="PKM72_09340"
     CDS             3016..3705
                     /locus_tag="PKM72_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNP61031.1"
                     /translation="MKRLFTVIGLGRFGYSVAQGLVTKGCEVLAIDKDEEKIQAISDI
                     ATFAVQCDATDERALKAVSAQNVDVAVVSIGENIEASILIVQTLKEMGVKSIVAKAVA
                     PIQGKILMNLGVDEVIYPERDAAVRLAHRLVSPSVLEYLELAPGFSVEEVSVSESLSG
                     LSLEDVKLRGQHNLNVIGIKKQVTRMVKGRMMKEEIFNFTPKPDDVIEKGDVLVMIGR
                     EEDLDRFCNNV"
     gene            4118..5257
                     /locus_tag="PKM72_09345"
     CDS             4118..5257
                     /locus_tag="PKM72_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNP61032.1"
                     /translation="MENQTVLDEPTETELATVKTEGIKPSGFGPALESLYFRSFGSRP
                     LLNKDSEIELAKEIDQASRAIRLIIKQSLHLTQPLKKGPQRDGTIALLKETFALSGLS
                     APAIEKLKSVLLDLGQTYPSVHPSIQDLCQQLQKAKFQLEKAKSELVQRNLRLVVDIA
                     KRYTGHGLAFLDLVQEGNIGLMKAAERFQYRKGFKFSTYATWWIRQGITRSLADQSRT
                     IRVPVHLNEISNKIARASKRLTQQYARPVQLDDIGEALKLSVEKVHDTIQAFQDPISL
                     ETPVGDGETFLTDFIPDMGTLTPDGPVSRQETNQQVERILDSLTPREQMVIRLRFGIG
                     QDEPWTLEEVGQSMSVTRERVRQIEAKALQKLKEPAMKDLLFSIK"
     gene            5964..6914
                     /gene="lpxC"
                     /locus_tag="PKM72_09350"
     CDS             5964..6914
                     /gene="lpxC"
                     /locus_tag="PKM72_09350"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNP61033.1"
                     /translation="MRHQQTLETSSFVSGIGLHSGNPASIAIHPAPVNTGVIFVMKHD
                     ADQIQSCPAGISFLGQTDHCTTLQAGDFGIQTVEHVLSALAGLEIDNAYVELLGTEIP
                     AADGSATPFVDMVYQSGILTQDVSRKYLKIVQPITVEDDHRSISVLPSALPQITYNID
                     FPHPLIQQQEYHHFCTPHEYSKNIAGARTFAFQKEVEFLWSRGLGLGGSLHNTVVFSD
                     TGLVNDEDLRFSDECVRHKILDLIGDLSLLGIPVIGHFIAKCAGHALHTQLVQAIMHN
                     PDKWIMLNAGETGKDGIFNGKNSSNSTIHLPELQPAFHAF"
     gene            7038..7583
                     /locus_tag="PKM72_09355"
     CDS             7038..7583
                     /locus_tag="PKM72_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61034.1"
                     /translation="MTMSLQSFTRPYPTISLRILFIICFCQFPWTPALSATPIENDPK
                     GFFGSLWGHSLRERSDLKQIDSLNSLQIYTIKLGAPQLGSIELESVKLYTIDGKYVRA
                     LFHYKGEATHNSLLQYLENEFGKSNDPYGSMIRGQQYNWRGPDTEIALTYHGFRDRGI
                     LAVESRIYAPLLLDSLSSGTL"
     gene            complement(7696..9339)
                     /locus_tag="PKM72_09360"
     CDS             complement(7696..9339)
                     /locus_tag="PKM72_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNP61035.1"
                     /translation="MGAIYRNVLEQIRMAFPQPVSDRVHDSYFVHSIMRAVEAVDAMK
                     SERPILGERGELDYHTARLSRLAEKGSSVEAVTQELVQYCSGLTVWGHPRTQQNVVPP
                     VTISSLIGVLLASLYNPNLAWDEYSHRVGLAEVEVVGMMAQLLGYDSELAGGMFTFGG
                     TGTTLYGMKLGLEKSLPGSMEHGIREDAVVFASDSSHYCRYNIAGWLGLGSHNLITIP
                     TTIHNEINLKVLREQAVQALQSGRKIVGMIATLGTTDAFGLDDLQGMVTLRNELVQEF
                     SLPYCPHIHADAVIGWAWSVFTDYPFEANPLGFRPRTVRALAGACKKIRHLSLADSIG
                     IDFHKPGFVPYISSLILVRQKEDLDLLQRPPDQMPYLYQFGEQRPGMYTLETSRSGGG
                     VLAALANLKLFGKEGLRVIIGHIVEMAQLLREHLESHECMTVVNRDNFGPVTLFRVYP
                     EGVDTFQIKEAEMHDVAFRDILLEHNAFNRKVFDFVHQEGMAGRGVILSLTDCYRHTD
                     YGEPIVALKSFILSPFVDENNVEAVVNKVLEARKLLTLS"
BASE COUNT         2583 a         2443 c         1964 g         2483 t
ORIGIN      
        1 cattcccaat actatggagg ataaccatga aacgtttatt tttctcccta acaatcgctc
       61 ttcttttctc gggatggtgg aatctcccca tcgttactgc tgagccctat cttttaacag
      121 ttcagcaact taaagcagga cttgaaaagt cttcccagtc caaacaaaaa ggatttgtgc
      181 tcatcgacgt tcggagtccg gacgaacata tgagtggctt cattcccgga acggatttca
      241 atatcgattt tcgagaaatg caaggacgac accgagagct caacgcgaaa ctggatcagc
      301 acatcgtggt gtactgccaa tccggacacc gcagcaatat tgctgccgaa accttaatgg
      361 ggttgggcta tcagaatgtc tataatgtgg aaggaagtat gaatgcctgg acagaagctg
      421 gatattctat tgaacacccc aagtaatcaa tgacagcctg ctgcaaagta cattagtttt
      481 ccgccacggt ggaatagatg ccgttaaagt tctggtgcat ctcccgaaag gcttccaaaa
      541 gaagtggatc aaaatgtgtc ggctttgtac ggtcgttccc gttcaaaatg atgtcgcatg
      601 tcttcgcatg ggtaaacgct gctttgtacg gtcgtcggct ccgtaaagca tcgtaaaggt
      661 cacacagcat cacaatccgc ccggttaggg gaatctgtgt tccctttaga cctcgaggat
      721 atccacttcc atcccaacgt tcatggtgag tcagcgcgac ttctttagcc atctctagca
      781 agggagacgt cgagccatcc aatagactcg ctcccaacaa aggatgttgc ttaatcgatt
      841 gccattcttc ttccgtcaat ggcccctgct tgccaagcac agcatctggc accccaattt
      901 tcccaacatc atgcattgga gcaaccgcaa ataagcaacg agcgcgtggc tgatcccagc
      961 caatagccaa cgcaagcgaa tgcgagtaat ggcttaatcg ttcaatatgc gcaccggttt
     1021 cctcatcttt atagcgtgac gcacgggcta agcgaagaat agtatcggca taggcctgct
     1081 ccaattcact attcttccgt tgttctgctt ccaatgacag tttaaggtcc ctggcgtagg
     1141 caagcatttg ggcatgagct tttttaagct gttgggtttt ctgaccttcc tgttccaaca
     1201 tctgcttgag atctttggca taggccatga gctgcaagcg agccgcctga agtttccctg
     1261 tggcagattc ttgggaagct tcgtcctttg aatgacccat taaggatgaa gaaggaatcc
     1321 catcctccgc gggcttaccc ttcctcatga ggcaagtacc tcgcgaactt tttgaatcaa
     1381 ctctaatggg ctgaatggct tgaccaaata gcctgcaagg ttaagggaag ccatctgctc
     1441 acgtgcatct atcccatctt tcgcggttaa cataacaatt ggaattctgg ccgtatcgga
     1501 gcgtaagcga atgtttcgca ccacctcaca cccatcgaga cccggcatca tccaatcgag
     1561 aatcaacaga tcgggtatgt gagtgcaaac tgcatccatg gctgcacaac catcaactgc
     1621 ggtgagaatc cgataggagg ggtgttcaag ggtgacttgg accaacagcc taagatcttc
     1681 ctcatcatcg gcaatgatga tcgtttttac gggacccacc atggtaatga tttcctgctg
     1741 gttggcccat tagggctcag ggagtatcta atccattgcc ggcatattca tcctccttga
     1801 tgagttgatc ctctgactgg gattcaggtt cgatagtatt cgattgctcc gtttgccaaa
     1861 tatttttttc gacttttctg gccacggtga taaaacctga atgggcaacc atacgatggt
     1921 caggccggac acttcggcct ttaatgttcc aagtccgcaa taacgtttca aaattttgaa
     1981 ccatggtaaa aacccggctc tgctccaaag cccgtaccgt ttgcatgacc tgcggcaccg
     2041 tcgggacata actcagatac agcccccccg aacgtaatgc cctggcggca tgaggaacta
     2101 ccctccatgg ctctggaaga tccaacacga cccgatcaaa catccaggaa gacgtccctt
     2161 ccccaatttc gataccttca taggcatcac gaatctgaag aaaatggttg ggaacctttc
     2221 ccataaaccg ttcgatattt ttcgtagcgg tagccgcaaa atcctcccgc atttcatagc
     2281 tcaccacaca cccccgatcc cccaccgcac gcagaagggc catggttaag gctcccgatc
     2341 ctacaccagc ttcaaagacc ctggcgcctg gataaatatc agcccacatt gggatgacgg
     2401 ccaagtcttt gggataaatc acctgagctc ctcgaggcat tttcaaggta tactcagcta
     2461 atgttggatg aacggcaacc attaacttcc cattggaaaa ctggaccaca gtaccatccg
     2521 gttttccaat aatgtcatca tgggcaatcc ggtctccact atgttggaag atttctccgg
     2581 ctttcagtgt gacggcatac ggccgctctt tcttgtcgat taaatgaacc cgttccccag
     2641 attgaaaatt tttcataagg cacgcatctt agcaaggctt ttcttcaatt tgatacccat
     2701 cataatggtg gagacttcat gaaaagtatg tcgcgacaac ccgccgacat ttcaatttga
     2761 atattcttga caccctccgc attcagacgt atgattgagt aacttcagtc gtgacatacc
     2821 tacgctacga atttttccaa ttgaccagat tctttaacct tgaaagtgcc ccccttgtgc
     2881 gtatgttctc aacatgcaaa acaccgaacc gccgctctaa aacggatcag aaggattccc
     2941 ttccacagca atttttgctg ctcttaaaac aggaatagtc gcgtggcgag aacagtagtc
     3001 gtgacctaat ctattatgaa acgccttttc acagtcattg gccttgggcg cttcggctat
     3061 agcgttgcac aaggattggt taccaaagga tgcgaagtac ttgccatcga caaggatgag
     3121 gaaaaaatcc aggcgatcag tgacattgcc accttcgctg tccaatgcga tgcgactgat
     3181 gaacgagcgt taaaagcggt aagcgcgcaa aacgtcgatg tcgcagtcgt cagtatcggt
     3241 gaaaacattg aggctagcat ccttattgtt caaacactca aggaaatggg ggtaaaatct
     3301 attgtggcca aagccgtggc cccaattcag ggaaaaattc taatgaatct gggggttgat
     3361 gaagttatct atcccgaacg ggatgcggcc gtccgtctcg cccaccgcct ggtttccccc
     3421 agcgtacttg aatacttaga gttggctccg ggcttcagtg tggaggaagt gtccgtttca
     3481 gagagcctgt ccggattaag tctcgaggat gttaaattac gcggacagca taacttgaat
     3541 gtgattggga taaaaaaaca agttaccagg atggtcaaag gacgtatgat gaaagaagaa
     3601 atctttaatt tcactcccaa accggatgac gtcattgaaa aaggtgatgt gctggttatg
     3661 attggccggg aagaagattt agaccgtttt tgcaataacg tgtaagaaat ataacagttc
     3721 tgtcaattag ctctgcactt tttccttccg catcttccac catcttgaaa cttgttagtc
     3781 tttccccttt cgcactatcc agggaattgg atgggctacc gatttcatta atagctaaac
     3841 tatcatctaa aaagtttata aaaatttcac ggatggcagt tttatccttt tgtttcaaat
     3901 tgttttacat ttcatgaaat gagaggctct tgccaaactg tcacaatgaa ggaaacccaa
     3961 cattgcaaaa tatggcattg agttgctttg cattgatggg tggaaggttt aaaacataac
     4021 attggcttgt tttttgcttt aaaaggatta tctaatgttc accttgttgc tgaactaaat
     4081 aggtcacctg aaaacataaa cccacaggga aactattatg gaaaaccaaa ccgttttgga
     4141 tgagcccaca gagaccgaac tcgctaccgt aaaaactgag gggatcaagc cttcgggatt
     4201 cgggccagct ctcgaatcac tatacttccg atcatttggc agtcggcctc ttcttaataa
     4261 ggattctgag atcgaattgg cgaaggaaat tgatcaggcg tccagagcaa tccggctcat
     4321 tatcaagcag agccttcatc tcacacagcc tttaaaaaag ggaccacaaa gggatgggac
     4381 cattgctctg cttaaggaaa catttgcctt aagcggcctt tcggctccag caattgaaaa
     4441 actaaaatct gtcctgttgg acttaggcca aacctatccc tcagtacacc catccattca
     4501 ggatctctgt cagcaacttc aaaaagccaa attccaacta gaaaaagcga aatccgaact
     4561 cgttcagcga aatcttcgcc tggttgtcga catcgcgaaa cgctatacgg gtcatggcct
     4621 agcctttctg gacttagtcc aagaaggcaa tattgggctt atgaaggcgg cagaacgatt
     4681 tcaatatcgc aaaggattta agttcagcac ctacgccacg tggtggattc gacaagggat
     4741 tacccgatca ctcgctgacc aatccagaac cattcgcgtt cctgtccatt taaatgaaat
     4801 ctccaacaag attgctcgcg cctcaaaacg attgacccaa cagtatgccc gcccggttca
     4861 gttggatgac attggagaag cactcaaatt gagtgtggaa aaagttcatg acaccattca
     4921 agcatttcag gatcctatat cgctagagac tccggttgga gatggagaaa cctttctcac
     4981 agacttcatt cctgatatgg gaactttgac gcccgatgga cctgtctcgc gccaggaaac
     5041 gaaccaacaa gtcgaacgga tcttggattc cctgactcca agggaacaaa tggtgattcg
     5101 cctccgtttt ggcattggcc aggacgaacc ctggaccctt gaagaggtcg gccaaagtat
     5161 gtccgttacc cgtgaacgtg tccgccaaat tgaagccaaa gcccttcaaa agttaaagga
     5221 acctgctatg aaggatttgt tattttccat taagtaaata gttgttgcaa actttttcta
     5281 cttacccatg aattctgaga cctcctgctt tgaatctgtt atttcaagca aggacttgtc
     5341 aatcgaccat tcaccgcttt aacatcggat aattcaactc ctgtttcatc tatgtgtaaa
     5401 gactagcgcc cttgttgcca tcagactcta ttttcaaact tcgacaagaa ttcagaagcc
     5461 accttcaaga attctacact catctcaatt tggcccctcc ctatcacagc atagaaaaag
     5521 cagtccagca tttttccaat actctcagga caagacctga agaattctgt cagggactgt
     5581 tacaagatac gtcaaaaaag tgggcagttt ttcaggaaat tttcctagcc tctggcttac
     5641 aaaaaaagca ccgaggaatc attgaacaat tagctctttc tccttattcg gctgtagctg
     5701 aagcccctga atcacgtcgc ttccttgaaa atttctcaac aaatccacac gaatccactt
     5761 cttaaccaac aattcacaca cggcattcca actcctcctc ctttaatcat taaaccctaa
     5821 ttagtggaaa agtatacatt tgtttggttt ctgcaacaca tcagaaacaa aatgattatt
     5881 ttgtccctaa aaacactgcc tttatctaat ccctcaatta gtataaattt tgcacattga
     5941 atacttaaga ttaccggttt tttatgcgtc atcaacaaac tctcgaaaca tcatcttttg
     6001 tatctggtat tggtctgcat tctggcaacc ctgcctcaat tgccattcac ccggctccgg
     6061 ttaacaccgg ggtgatcttt gttatgaagc atgatgctga tcaaattcaa tcatgccctg
     6121 ctggcatttc atttttaggc cagacagatc attgcaccac acttcaagct ggtgattttg
     6181 gcatacagac ggttgagcat gtgttaagtg cactagccgg cctggaaatc gataatgctt
     6241 acgtggaatt gcttggcacc gaaatcccgg cagcagatgg aagcgccaca ccatttgtcg
     6301 acatggttta ccaaagcggt atcctcactc aggatgtatc acgcaaatac cttaaaattg
     6361 ttcaaccaat cactgtggaa gacgatcacc gatccatcag tgtccttccc tcggcacttc
     6421 cccaaataac ctataacatt gatttccctc atccccttat acaacaacaa gaatatcacc
     6481 atttttgtac gcctcatgag tatagtaaaa atattgctgg agcccgtacc tttgcctttc
     6541 aaaaagaagt cgaattttta tggtcccgag gactggggct cggggggtca ctacacaata
     6601 cggtcgtttt ttctgatacc ggattggtca atgatgagga cctacgcttt tcggacgaat
     6661 gcgttcgcca taaaattctc gatctcattg gcgacctgtc gctattagga attccggtta
     6721 ttggtcattt cattgcaaaa tgcgcgggac acgcgctcca tacccaattg gtccaagcca
     6781 tcatgcataa ccctgacaag tggattatgt taaatgctgg agaaaccgga aaggatggca
     6841 tcttcaacgg aaagaattcc tccaattcaa cgattcacct accggaactc caacctgctt
     6901 ttcatgcgtt ctgattccgt ctcccatttg ccatggtatg cccttaacaa tccttgcgtt
     6961 tgatatctgc tcatcaacaa ataggaagaa atcattttct ccattgtctt catcccctgc
     7021 gcgtggtagc gtctgggatg accatgagtc tccaatcatt tacccgcccc tatcctacaa
     7081 tcagccttag aatacttttc ataatttgct tctgccagtt cccgtggaca cccgccttaa
     7141 gtgctacgcc aattgaaaat gatcccaaag ggtttttcgg cagcctctgg ggccattctc
     7201 ttcgggaacg ctcagatctt aagcaaatcg actcacttaa ttccttgcaa atctatacaa
     7261 ttaaactcgg agcaccccag ctcggatcca ttgagttgga atccgtcaaa ctctatacaa
     7321 tagatggaaa gtatgttcga gccctcttcc attacaaagg agaagctacc cataattccc
     7381 tcttgcagta tctggaaaat gaatttggaa aatctaacga tccctatgga tccatgatcc
     7441 gtggtcaaca gtacaactgg cggggtccag ataccgaaat cgcgcttacc tatcatgggt
     7501 ttcgagatcg gggaatatta gctgtcgaga gcagaattta cgcaccgttg cttcttgact
     7561 cactctctag cggtactctt tagcacctca attttcttga ccattaacaa aaaatcatca
     7621 aatttggcac tcattagcct tcgcatacct cacacagagc caagcatgtg aaattccgtc
     7681 tacctctcac ctcatttagg acaacgtcag caactttctg gcctctaaca ccttattaac
     7741 caccgcttcc acattgtttt catccacaaa gggcgaaaga ataaatgatt tcaaggccac
     7801 gattggttcc ccataatcgg tatgacggta gcaatctgtc aatgagagga ttactccacg
     7861 cccggccatg ccttcctgat gcacaaaatc aaaaaccttt cgattgaacg cattgtgttc
     7921 caacagaatg tcacggaacg cgacatcatg catctccgct tcctttatct ggaatgtgtc
     7981 caccccttcg ggatacaccc gaaagagagt gacggggcca aagttatccc ggttgaccac
     8041 cgtcatacat tcatgactct ccaggtgctc cctcaaaagt tgcgccattt ctacaatatg
     8101 gccaatgatg actcgcagcc cttctttccc aaacaatttc aagtttgcca gggcagcaag
     8161 cactccccca cccgatcgtg atgtctccaa ggtatacatg ccgggccgct gttcaccaaa
     8221 ttggtacaga tagggcatct gatccggggg acgttgtagc aaatcgagat cttctttttg
     8281 ccgaacgaga attaagcttg aaatataggg gacaaaccct ggcttatgaa aatcaattcc
     8341 aatcgaatct gccaaagaga gatgtcgaat ttttttgcag gctccagcca aggcccgcac
     8401 agtccgagga cggaacccta acggattcgc ttcaaaggga tagtcggtaa agacggacca
     8461 tgcccatcca atgactgcat ccgcatgaat atggggacaa taaggcagag aaaactcctg
     8521 gacgagttca ttccgcaacg tcaccatacc ttggagatca tccagcccga atgcatccgt
     8581 tgtccccagc gtggcgatca tgccgacaat ttttcgaccc gattgtaagg cctgtacagc
     8641 ttgctcccga agcaccttga gattgatctc attgtgaata gtggtaggaa tggttataag
     8701 attatgactc cccaacccca accaccctgc aatattgtaa cgacaataat gactactatc
     8761 gcttgcaaac accaccgcat cttcacgaat cccgtgctcc atggaaccag ggagtgattt
     8821 ttccaagcct aatttcatgc cgtacagcgt ggttcccgtt ccgccaaaag taaacatccc
     8881 cccggctagc tcagaatcat agcctaataa ttgagccatc atcccgacca cttccacttc
     8941 agctaaaccc acccgatgac tatactcatc ccacgccaaa ttggggttat agagtgaagc
     9001 gagcaacaca ccaattagag aagagatcgt aacgggggga acaacatttt gctgtgtccg
     9061 cgggtgcccc cagacagtga gccccgaaca atactgcacc aactcctggg tgacagcctc
     9121 aacactgctg cccttttcag ctaaccggct aagccgagcc gtgtgataat ccaattcacc
     9181 acgctcaccg agaatcggtc gctcactctt catggcatcc actgcttcaa cggccctcat
     9241 gatgctatga acaaaatagg aatcatggac tcggtcagac accggctgag gaaaggccat
     9301 gcgaatctgc tctaagacgt ttctatatat agctcccatg tctccagact cctctcaatg
     9361 ccttgctggc ctcatagggt gatcgttctc tctccaggcc aattttacgt aacccccttg
     9421 actcctcttg aaacaaggtt cactataggc ccgttttttt aacgtcatcc tag
//
LOCUS       DAORAQ010000099         9377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1168_length_9377_cov_64.042480, whole genome shotgun sequence.
ACCESSION   DAORAQ010000099 DAORAQ010000000
VERSION     DAORAQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1168_length_9377_cov_64.042480"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PKM72_09365"
     CDS             <1..1209
                     /locus_tag="PKM72_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNP61036.1"
                     /translation="LVPRFHYKAARPDGTIIENDGEGESPRTLRSQLEAQGLLVLDLA
                     DSGTRKIVSGKKRQRPLALRDFLIFNQEFLALLKAGLPMLRCFDLLAERNTQGGFQQA
                     LQSVREHIRGGSAISEAMTHHPTYFPELYQASLRAGEQAGNLPEVLSRYIAYLKMLIG
                     VREKVVKATIYPVFLLGFGLLVTIGLLFFVLPSFAEVYKESRVELPWATQLLIDLTQS
                     ATQWLMGLLILMGLGGIVIYWWKNTPQGQWQIHWLYLHAPILGPLFLKNQVIRLARTL
                     STILGGGIPLLSALQVTAKSMPNKVFAQALQSVIVHVREGTSLSSALKKENFLPRLTL
                     EMVEVGETTGSLEAMLFEVAEFHEGELDFQLSRLMTWIEPMFIILIGVILGGVVIALY
                     LPIFQMAGAV"
     gene            1240..2967
                     /locus_tag="PKM72_09370"
     CDS             1240..2967
                     /locus_tag="PKM72_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNP61037.1"
                     /translation="MTTPVKRMPFEDLLVEKGLIRERDKETLLETARTTNQTIAAILL
                     EQGTLTEEVIGQALADQYGLPWNNLKEFRIPSSLMESIPVELMHRYEFVPIEQEGPIL
                     TIAIAKPQDLRIIDELEHQLGFELRLVVSSHSAILEALGNSEGNSQVLTRIKAELDPV
                     LIKEDEKGEEVLSVERITKDLSPVVKLVNTIILTALQKQASDIHIEPTERTVDVKFRI
                     DGVLYLAMEPFDTGIHPSLISRLKIMSELDIAERRTPQDGRFKLLLNHRTIDFRVSIL
                     PSVYGESVVIRILDKQHISAGVQGLRLEALGYTGEDLRRFRRAIIRPYGMVLVTGPTG
                     SGKTTTLYAAINEVHSDEDKIITIEDPVEYQLKGIVQIPVNEKKGLTFARGLRSILRH
                     DPDKIMVGEIRDVETAQIAIQSALTGHLVFSTVHANNAFDVISRFVNMGIESYNFVAS
                     LSCILAQRLVRSICPTCRVPIQIHPEQCEESGIEYEDVKHLTFYEGKGCNECHGLGFR
                     GRKAITEFLDMTDSIKEMILNEKSISEIRTAAMAQGMSTLRQAALEKVYRGETTLKEI
                     NRVTPIEEI"
     gene            2967..4145
                     /locus_tag="PKM72_09375"
     CDS             2967..4145
                     /locus_tag="PKM72_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61038.1"
                     /translation="MHFFPISAPTVALSISEDGLCLVEIKKSWRKKSLRHIARLPLSP
                     EVLTLSSAKPNIANPNEFLTQLKALVKPYRRPLSIALSLPDICARTSIFDFASFPTKK
                     PEQTALVNWRFQQDLKLDTSQSRLAFGLYVPSSVRDSSPGNRGQVRVLGTAIRHEIIE
                     QYENMCLEAGLIPTSVGMSGLDIFDLYRHNIQDMLTAENPSQASAPPVVMFLFISHWG
                     FTFLACQEGSPRFVRTKALGIKTEANHSQDSSVNPEAEEDRETREHCTTPSEDLISNA
                     PQESDTGNNPYSSYIAMKVEKEILATLQYYFEMFPISATASSPVNLFMTTDLPYGQSL
                     MPPLDHIQQSLKASGGHEPQIRVTSLSHASHFHLLNRHLAASNREEAALPGYASLMVA
                     "
     gene            4142..4756
                     /locus_tag="PKM72_09380"
     CDS             4142..4756
                     /locus_tag="PKM72_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNP61039.1"
                     /translation="MMIPRVHRNLSAPGIHFLQLSQLGLTLLTAGTLALTASFWWQGK
                     SLHEQIDILEDQASSIETANNQLVSQARVAGLDLSHQAIRSIPSKVNFVKQVRERVGF
                     SWTQLLTDLETAVPSDITVNSVSLDEKTDTILLQGSAASLPDLNRLIHQLDKHAAFQN
                     VLLSQHSTKNTKEDQGGSHSVFTLTVTYDPRRSTSHLTGVPRQR"
     gene            4753..5325
                     /locus_tag="PKM72_09385"
     CDS             4753..5325
                     /locus_tag="PKM72_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61040.1"
                     /translation="MNSFQIIQRSKTTIRITKFLSPLFIVTILAGLSSVGMFAFIFYP
                     AQDRFEHITALNQASLHTQKTMQTARQTQTILSTIWNALPDSGEFTDLSLDIADLAKR
                     NRVQIPGMGYDFRPLPHKLATKGTFAFEAVGPYEAIRKFIFELEQRWPYLFIEKLSVE
                     TSKKQKGVIFKLKVSTFLKEFPEPVKKTKA"
     gene            5325..5924
                     /locus_tag="PKM72_09390"
     CDS             5325..5924
                     /locus_tag="PKM72_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61041.1"
                     /translation="MTNQRKWLMLGGLLLAWGMVTIFQLWDSEPTDPMLHSATSENPL
                     PPLKDLKLDPDFPNPRQTVTFSLPRNIFAPLGMAQIARQDTNKATTSRLAKAAPSPTP
                     PPPQPRPPGPSFADLAGQRAREQLNQFRFLGYLTKGGESQAFLTNGQAIYIVKQGEIL
                     DGRVQVHRIEPETVVLSTQVWETGSHVQATIPLTPDTSG"
     gene            5973..6719
                     /locus_tag="PKM72_09395"
     CDS             5973..6719
                     /locus_tag="PKM72_09395"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNP61042.1"
                     /translation="MFESRSVFFLRNPPDTALWWLVGVLAYCVLWVSPVISATYDCLD
                     ESGARVLTDNPVQLFNCTQLPSQSSTLTNQTPSTVPTLQATTKSIPNQAPPDTQISIG
                     LSSLDGENQDRPFNEDITIPLTKSGGSFIVPVVLNQERSAQLIVDTGASMTVLSNQVA
                     IDLGILGTTDNELLTVNTAGGSVQVNMNYLSSISVGTAQATNVAVALHDLPDIPEHIE
                     GLLGMSFLKHFLVTLDAEHAQLILRPKQKP"
     gene            complement(7009..8064)
                     /locus_tag="PKM72_09400"
     CDS             complement(7009..8064)
                     /locus_tag="PKM72_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNP61043.1"
                     /translation="MRVQHMPTTFRLALFFYALFLCVLPGLAFANTEELSPSEAIAPS
                     VWQVALDGSGHFTSIQEAIDQAASGDTILIKQGAYAEDVTVHSKEGLSIIGEGMDLVV
                     LTGKKRVGTLHIGKWPYGATNVTIQDLSVIQHGGLGVGIFNGSGVRLTRIRVNGMVFS
                     QQVQDVLLEDCVIGDSETTGVAFADSTGTLSGNLIYHNDHGVAIGGNSDVTLRQNVIT
                     RSLYEAVLMTDQSKARLIQNTLAQNGGGAAFHDDAQADVQGNIISQSAVGFLFFPGSH
                     TTLAFNALSSNQGDYVMTGTPPTPAPDRAGKTDVQFAPGFVSPENGDFRLQSDSSLIQ
                     VGAFPYLGALPPVSSNP"
     gene            8226..8864
                     /gene="coaE"
                     /locus_tag="PKM72_09405"
     CDS             8226..8864
                     /gene="coaE"
                     /locus_tag="PKM72_09405"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNP61044.1"
                     /translation="MVVVGLTGGLASGKTTVAKIFQSLGARIIDADELARAVVKPGKP
                     AWKNIVKEFGPQVLAKDHTINRQALAEIVFKSPKKLHVLQAIIHPRVAREQAKQVKSM
                     RHENPHAVIIYDAALLLEAGAHKRMDHVIVVTADRTTQLARACRRDGLTKAQALRRIR
                     QQMPLRRKLDYADAVLDGTWSRTRLRHVVQSLYRTYDSEARSRTTRRSLNTP"
     gene            8903..>9377
                     /locus_tag="PKM72_09410"
     CDS             8903..>9377
                     /locus_tag="PKM72_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP61045.1"
                     /translation="MVHDVIILGSGPAGLTAAVYTARANLSPLLIDGSQPGGQLTITT
                     DVENFPGFPKGIMGPQLIQDMRAQAERFGTQFRQGHVTSVDLQVRPFKVTVDDEATLE
                     TRTLIIATGASANLLGLPSESRLMGHGVSTCATCDGFFFRGKEIAVIGGGDSAVEE"
BASE COUNT         2443 a         2551 c         2152 g         2231 t
ORIGIN      
        1 ctagtgcccc ggtttcatta caaagccgct cgtccggatg gaacgattat tgaaaacgat
       61 ggggaaggcg aatcacctcg aacccttcgc tctcaactcg aggcacaagg actgcttgtt
      121 cttgacctgg cagactctgg caccagaaag atcgtctcag gtaaaaaacg gcaacgccct
      181 ttagcgctca gagatttcct gattttcaac caggaatttt tagcgttact caaagccgga
      241 ttacccatgt tacggtgttt tgatctcctg gcagaacgga acacccaagg gggatttcaa
      301 caagccttac aatcggtccg agagcatatt cgaggaggat cggccatttc tgaagccatg
      361 acccatcatc caacctattt ccccgaactc taccaggcct cactacgtgc cggagaacag
      421 gcaggcaatc tgccggaagt cctgagccga tatattgcct atttaaaaat gctaatcgga
      481 gttcgagaaa aagtcgtcaa agccactatt tatcccgtat tcttattggg atttggactc
      541 ctggtgacca tcggcctgtt attttttgtc ctaccctcat ttgcggaagt ctataaagaa
      601 agtcgcgtgg aattgccctg ggccactcag ctcctgattg atctgacgca atcggctacc
      661 caatggctga tgggacttct aatcctcatg gggcttgggg gcatcgtaat ctattggtgg
      721 aaaaacacac ctcaaggtca atggcaaata cattggttat atctccatgc gccaatactt
      781 ggtcccttgt ttttgaagaa ccaggtcatt cgtttggcca gaacgctgtc aaccattctt
      841 ggaggtggga tcccgttgtt atctgcgctt caggtaacag cgaaatccat gcccaataaa
      901 gtctttgccc aagcgctcca atcagtcatc gttcatgttc gagagggtac cagcctctca
      961 tcggcattga aaaaagagaa ttttctccca cgcctcaccc tggaaatggt tgaagtggga
     1021 gaaaccaccg gttcacttga agccatgctc tttgaggtcg cggagtttca tgaaggggaa
     1081 ttggactttc aattatcccg actcatgact tggatcgaac ctatgttcat tattctgatt
     1141 ggggttattt taggaggggt ggtgattgcc ctctatcttc ctatttttca aatggcaggg
     1201 gccgtgtaac gaccaagtgg ccttaacgaa agaataccca tgaccacacc cgtcaaacgc
     1261 atgccattcg aagacctctt ggtggaaaag ggtctcatcc gggaacggga taaggagact
     1321 ctgctcgaaa ccgcacgcac gacgaaccag accattgcgg ccattttgtt ggaacaaggt
     1381 acgctcacgg aggaggtgat cggccaggct ttggctgatc aatatggatt gccctggaac
     1441 aatctcaagg aatttcgtat cccctcatcg ctgatggagt ccatcccggt cgaactcatg
     1501 catcggtatg aatttgtgcc catcgaacaa gaaggcccga ttctgacaat cgcaatcgcc
     1561 aaaccacagg acctgcgaat cattgatgaa ctggaacacc aattgggatt tgagttgcga
     1621 ttggtcgtct cgtctcactc agccattctt gaagccttgg gcaatagcga aggcaacagc
     1681 caggtgctca cgcgcatcaa agccgaactg gatccggtcc tgatcaaaga agacgagaaa
     1741 ggggaggaag tcctatccgt tgaacggatc accaaggacc tcagcccggt agtcaaactg
     1801 gtgaatacga ttatcttaac cgccctgcaa aagcaggcga gtgatataca tattgagccc
     1861 accgagcgca cggtcgatgt gaaattccgt attgacgggg ttctctattt agcgatggaa
     1921 ccatttgata ccggcattca cccctctctt atttccaggc tcaaaatcat gtccgaactg
     1981 gatatcgcag aacgtcgcac gccgcaagat ggccggttca aattacttct gaatcaccgg
     2041 accattgatt tccgggtttc cattcttcca agtgtgtacg gggaatcggt ggtcatcaga
     2101 attttggata aacaacatat ttcggcgggc gtccaagggc tgcgcctgga agccctgggc
     2161 tataccggcg aggatctgcg gcgtttccgc cgagccatca ttcgtcccta tggcatggtc
     2221 ctggtcaccg gacccacggg aagcggaaaa accacaaccc tgtatgccgc tatcaatgag
     2281 gtgcactccg atgaagataa aattattacg atagaagacc cggtggaata tcaattaaag
     2341 gggatcgtac aaattccagt caatgaaaag aaggggctca cctttgccag agggcttcga
     2401 tccattttgc gacacgaccc tgataaaatt atggttggcg aaattcggga tgtagagacg
     2461 gcacaaattg ccatacaatc ggctctcacg gggcatttag tctttagcac cgtgcatgcg
     2521 aataatgcct ttgatgtcat tagccggttt gtgaatatgg gcattgaatc gtataatttt
     2581 gtggcttcct tgagttgcat tctcgcccag cgtctggtcc gatccatttg cccaacctgc
     2641 cgtgtgccaa tccagattca ccctgagcaa tgtgaagaat ccggcattga gtatgaggac
     2701 gtcaaacacc tgaccttcta tgaaggcaaa ggctgcaatg aatgtcacgg cttgggcttc
     2761 cgcggccgaa aggccattac agaattctta gatatgaccg attccatcaa ggaaatgatt
     2821 ttaaacgaga aatccatatc tgaaattcgc acggctgcca tggcccaggg gatgagcacc
     2881 ttacggcaag cagcgcttga aaaagtctat cgaggagaaa ccacactcaa ggaaattaat
     2941 cgggtcactc cgatcgagga aatctgatgc atttttttcc catttcggca cccaccgtgg
     3001 cactctccat ttctgaggat gggctatgcc tggtggaaat caaaaaatct tggcgaaaaa
     3061 agtcgctccg gcatatcgcc agactcccgc tctcgccgga agtcctcacc ctctcttcgg
     3121 caaaacccaa cattgccaat ccaaatgaat ttctcactca acttaaggca ctggtcaagc
     3181 cctatcgtcg cccgctctcc attgcactca gtctccccga catatgcgcc cggacaagca
     3241 tttttgattt tgcatccttc cccaccaaaa aaccggagca aacggcactg gtgaattggc
     3301 gttttcaaca ggaccttaaa ctcgatacct cccaatcgcg cctggctttt ggcctctacg
     3361 tgccctcatc ggtcagggat tcctccccag gcaacagagg ccaggtcaga gtgttaggca
     3421 cggccattcg ccatgaaatt attgaacaat atgagaacat gtgcttagaa gccggcctga
     3481 tcccaacttc tgtgggaatg tcggggctcg atatctttga tctgtaccgc cacaacatcc
     3541 aggacatgct cacggctgaa aacccctctc aggcatccgc gcccccagtc gtcatgtttc
     3601 tcttcataag tcattggggt tttacttttt tagcttgtca ggaaggcagt ccacgcttcg
     3661 tccgcacgaa agctcttggc atcaaaacgg aagcaaatca ctctcaagat tcttccgtga
     3721 atcctgaggc tgaggaggac agggaaacca gagaacattg cacaacaccg tcagaagatc
     3781 tgatttctaa cgcaccccaa gaatccgaca cagggaataa tccctattct tcgtacattg
     3841 ccatgaaggt agaaaaggaa atcctggcaa cactgcaata ttatttcgag atgtttccca
     3901 tctctgccac ggcatcttct ccggttaatc tgttcatgac aaccgacctg ccctatggac
     3961 agagccttat gccgcctctc gaccacattc aacagtccct gaaggcaagt ggaggacatg
     4021 agcctcaaat tcgagtcacc tcgctttctc atgcctcaca ttttcattta ttaaacagac
     4081 acctggcggc gagcaaccgg gaagaagcgg cattgccagg ctatgccagt ctgatggtgg
     4141 catgatgatt ccacgtgtac ataggaattt gtctgcaccc ggcattcatt ttcttcaatt
     4201 gtcccaactc gggctcaccc tcctcacagc cgggacactc gctcttaccg cttcattctg
     4261 gtggcaggga aagtcattac atgaacagat tgatatccta gaagaccaag catccagtat
     4321 cgagacggcc aacaatcaat tggtctctca agccagggtg gctgggcttg acctttcgca
     4381 tcaggccatt cgaagcattc catcaaaagt caattttgtg aaacaggttc gtgaacgggt
     4441 tggattttcc tggacacaat tactgaccga tctggaaaca gccgttccct cggatatcac
     4501 cgtgaactcg gtgtctctcg atgaaaaaac cgacacgatc cttcttcaag ggtcggcagc
     4561 ttccctacca gacctcaacc ggctcattca tcagctagac aagcacgcag cctttcagaa
     4621 tgtcttgcta tcgcagcatt ccacaaaaaa caccaaagag gatcaaggtg gctcccatag
     4681 cgtgtttacg ctcacagtca cgtatgatcc ccgacgctcg acctctcact tgacaggggt
     4741 tcccaggcaa cgatgaattc ctttcagatc atacaacgat caaagacgac gatccggatc
     4801 acaaaatttt taagtccgtt gttcatcgtg acaatccttg caggactgtc atcggtcggg
     4861 atgtttgctt tcatctttta cccggcccag gaccgttttg aacatatcac ggccctgaat
     4921 caagcttccc tgcacacaca aaaaaccatg cagacggcca ggcagaccca aaccattcta
     4981 tccaccatat ggaacgcgct tccggattcc ggagagttca cagatttaag tttagacatt
     5041 gccgatttag caaaacgaaa tcgcgtacaa attccaggta tgggatacga cttcagaccc
     5101 ctcccccata aactggccac aaaaggcacg tttgcgtttg aagccgtagg accctatgag
     5161 gctattcgta aattcatttt cgaactagag caacgctggc cctacctctt tattgaaaaa
     5221 ctgtccgttg aaacatctaa aaaacagaaa ggggtcatct ttaagctaaa ggtctctacc
     5281 tttctgaaag aatttcctga accggtcaaa aagacgaaag cctaatgaca aatcaacgaa
     5341 aatggctaat gttggggggc ttactattgg cttggggaat ggtgacaatc ttccaattgt
     5401 gggattcgga gccaacagat cccatgcttc actctgccac gtccgaaaac ccacttccac
     5461 cacttaaaga tttgaagtta gacccggatt ttcctaatcc tcgacaaact gtcacgttct
     5521 ctctccctcg aaatattttt gcacctctgg gcatggccca gatcgcccga caagacacaa
     5581 acaaagccac cacctctcgc ctcgccaagg cggcaccctc cccgacgcca cctccacccc
     5641 aaccgcggcc cccaggtcct tcttttgccg atcttgcagg acagcgagcc agggaacaac
     5701 tcaaccaatt ccggttcctg gggtatttaa ccaagggggg ggaaagccag gccttcctca
     5761 ccaatgggca ggccatttat atcgtcaaac agggagagat cttggacggt cgtgtgcagg
     5821 tgcacaggat cgaaccagaa accgtggtcc tgtccaccca ggtgtgggaa accggctctc
     5881 atgttcaggc caccattccc ttaacacccg acaccagcgg gtaatcggct tcatagcgtt
     5941 ttcccctcgc atattggtat ccttttccca ttatgtttga aagtcgcagt gttttttttc
     6001 ttaggaatcc tccggacacc gccttgtggt ggcttgttgg agtgttggca tactgtgttc
     6061 tatgggtatc tccagtcatt tcagcgactt atgactgtct cgatgaatcc ggggcacgtg
     6121 tattaacaga caacccggtc caactattca actgcacgca attgccatca cagagctcga
     6181 ctctcaccaa tcaaacacca tccacggttc caacacttca agctaccacc aaatccattc
     6241 ctaatcaggc acctcctgac acacagatct ctatcggctt gtcctccctg gatggggaga
     6301 atcaggatag gcctttcaat gaggatatca ccatccccct caccaaaagt ggaggatcat
     6361 ttatcgttcc ggtcgtgttg aatcaggaac gatcggccca acttattgtg gataccggtg
     6421 cctccatgac agtcctttca aatcaggtcg ccattgatct cggaatcctt ggaaccaccg
     6481 acaatgaact cttgaccgtc aacacggccg gggggtcagt ccaagtcaac atgaattatc
     6541 tctcctcaat ctcggtagga acggcacaag ctaccaatgt cgccgtcgcc cttcacgatc
     6601 ttccggatat tccggaacac attgagggtc tgctcggcat gtccttcctt aaacactttc
     6661 tcgtgacgct ggatgcggag catgctcaat tgattctccg tcccaagcaa aaaccctaat
     6721 ctcatatacc gtcgttccgg gatgttcaga atctcggggt ctgtgtggca ttgcgtaggt
     6781 acaaagcgag agagggatag attatggatt tctgcgccaa ggatgtccaa ccacccaaaa
     6841 gactcttgaa ggtttcaaat atttggtttt ctcattcacg catgggtaag aaaaaacaat
     6901 cggagaacat tcatggagtg ttgaaaaaga ctcgtcccgt tatgcactga gacctctcga
     6961 agagaaggcc gcagacactt ggacagacag acgaaactga aaaggaattt acgggttgga
     7021 agaaaccgga ggcagagccc ctaaatacgg gaaggcacca acctggatca aggaagaatc
     7081 ggactgaaga cgaaaatccc cgttttcagg actgacaaat ccgggagcaa attgtacatc
     7141 ggttttccct gccctatccg gagccggggt gggtggagtt ccggtcatga cataatcccc
     7201 ctggttgctg gacaaggcgt taaaggccag cgtggtgtga ctccccggaa aaaataaaaa
     7261 acctactgcg ctctgactaa taatatttcc ctgtacatcg gcttgcgcat cgtcatgaaa
     7321 agcagcccca cctccattct gagccagggt attttgaatc aaccgggctt tagactgatc
     7381 agtcatcaac acggcttcat aaagacttcg ggttatcaca ttctgtctga gggtcacatc
     7441 agagttgccc ccgatagcca cgccatggtc attatggtaa atcagattgc ccgatagggt
     7501 gcctgtagaa tccgcaaatg ccacacctgt tgtttcactg tcaccaatca cacaatcttc
     7561 caaaagaaca tcctgcacct gttgactaaa caccatgcca ttgacacgaa tccgtgttaa
     7621 tcgcacgcca ctgccattaa aaatgccaac ccccaatcct ccatgttgaa tcacggagag
     7681 atcttgaatc gtcacattcg ttgccccata gggccatttg ccaatatgaa gagtcccgac
     7741 acgctttttt ccggtcagaa ctaccaggtc catgccttca ccgataatcg acaaaccctc
     7801 cttgctatgc acagtaacat cctcggcata ggctccctgt ttaatcaaaa tcgtatcacc
     7861 tgatgcagcc tgatcaatcg cttcttgaat cgaggtgaaa tgcccagacc catccaaggc
     7921 aacctgccat accgacgggg caattgcctc ggaaggggaa agttcctcag tattggcaaa
     7981 cgccaatcca ggcaggacac acagaaacag ggcataaaaa aagagagcta gtcgaaaggt
     8041 ggtcggcatg tgttgaactc tcacggtgtt aattccccct tgtcaagctc ttggcagtcg
     8101 atcggctcac ccaaaggaaa ggactctcat cgagtcaata acccaagggg tttggaacgg
     8161 tcaagcaaat gtcaaaaatt ttcctcattt attgtcgctc ccaaccccct atgctaaggt
     8221 tggccatggt cgttgtcggg ttaactggag gattggcatc aggaaaaacg accgtcgcca
     8281 aaatatttca atcacttgga gccaggatta ttgacgccga cgaactggcc agagccgttg
     8341 tcaaacctgg aaaacctgct tggaagaata tcgtcaagga attcgggcca caagtgcttg
     8401 ccaaagacca caccattaat cgccaagctc tcgccgaaat tgtgtttaaa tcccctaaga
     8461 aattacacgt tttgcaagcg atcatccatc cccgtgtcgc ccgcgaacag gccaaacagg
     8521 ttaagagcat gcgacacgaa aatcctcatg cggttatcat ttatgatgcc gcgttattat
     8581 tggaagccgg tgcacacaag cgtatggatc atgtcattgt ggtcaccgcc gaccggacca
     8641 ctcaactcgc tcgcgcctgc cgtcgtgatg gcctgaccaa ggcccaagcc ctccggcgaa
     8701 tcagacaaca aatgcccctt cgccgcaagc ttgactatgc cgacgccgtg ttggatggaa
     8761 cttggtcacg tacccggttg cgccatgtgg tccagtcatt gtataggaca tatgacagtg
     8821 aggcgaggtc acgaacgacc cgtcgcagcc tcaatacccc atagaccgtt cacctacatg
     8881 tctaactgta aggagttcac gcatggtgca tgacgtcatt attctgggtt cgggcccggc
     8941 tggattaacc gccgccgtct ataccgctcg agccaatctt tcccccttgc tcattgatgg
     9001 ttcgcagcct ggcggacaat taaccatcac caccgatgtg gaaaatttcc ccggattccc
     9061 caaaggcatc atgggccctc aactcattca agacatgcgg gcccaagccg aacggttcgg
     9121 cacccaattt cgccaggggc atgtcaccag cgtagatctc caggtgcgcc cctttaaggt
     9181 cacggttgat gatgaagcca ctctggaaac tcgaactctg attattgcga ccggagcctc
     9241 ggcaaattta ctaggcctac cctccgaaag ccgcctcatg ggtcatggcg tctcgacatg
     9301 tgccacctgc gacgggttct tctttcgtgg caaagagatt gcggtcatcg gcggcggtga
     9361 tagcgccgta gaagaag
//
LOCUS       DAORAQ010000100         9373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1169_length_9373_cov_64.798562, whole genome shotgun sequence.
ACCESSION   DAORAQ010000100 DAORAQ010000000
VERSION     DAORAQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1169_length_9373_cov_64.798562"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..725)
                     /gene="purQ"
                     /locus_tag="PKM72_09415"
     CDS             complement(12..725)
                     /gene="purQ"
                     /locus_tag="PKM72_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNP61046.1"
                     /translation="MNIGIIVFPGSNCDRDCGYVLSKIMKQTAHFIWHQERSLKGMDA
                     IILPGGFSYGDYLRTGAIARFSPIMDAVVDFAGRGGMVLGICNGFQMLLELGILPGAM
                     LRNRDLSFICEDRYIRVENANTPFTNHCEPGQILKLPIAHAEGNYYSNPVTLSALQAN
                     AQIVFRYCDREGKVTSQANPNGSLDSIAGIRNAAGNILGLMPHPERGAEHLLNNEDGL
                     YIFRSMISALEKHKTPQTV"
     gene            complement(821..1150)
                     /gene="purS"
                     /locus_tag="PKM72_09420"
     CDS             complement(821..1150)
                     /gene="purS"
                     /locus_tag="PKM72_09420"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNP61047.1"
                     /translation="MAKAILATTLAVTSSLYSPNAWRVFCEIMKAKIYITLKNGILDP
                     QGRAIQQSLQTLGFDTVGEVRIGKFIEVDLCETDRTSAGNSIKEMCEKLLANTVIEDY
                     QYEIIEA"
     gene            complement(1182..1871)
                     /locus_tag="PKM72_09425"
     CDS             complement(1182..1871)
                     /locus_tag="PKM72_09425"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF006868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNP61048.1"
                     /translation="MFYEGKAKKIFLTEKLDEVIHYFKDDATAFNAQKRGTILEKGVV
                     NNKISATLFQYLAEAGTPSHFIQQLNDREMLTRRVKIIPVEVVVRNRATGSIVKRLGL
                     EEGLIIDPPLVEFFYKDDSLGDPLISEDHIRLLKLATPPQVEELRQQALKINEVLKPF
                     FQKRGMMLVDFKLEFGLWNGQIILADEISPDTCRFWDIQTGERMDKDRFRKDLGRIEE
                     TYQEVLKRVCG"
     gene            complement(1883..3253)
                     /gene="purB"
                     /locus_tag="PKM72_09430"
     CDS             complement(1883..3253)
                     /gene="purB"
                     /locus_tag="PKM72_09430"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNP61049.1"
                     /translation="MIDRYTLPRMGAVWTQTHKYELWLEVELAVCEAMEKMRQIPPGV
                     AARIRKKVKVNPLRIAAIEQVTKHDVIAFLESVAEQAGNDGRFLHVGLTSSDIVDTSL
                     SLQLVEAAQLIREDVKALLDALRDLAFAHQDTLMVGRSHGIHGEPISFAWKVAIWYQE
                     FQRQQIRLEAAFVDIAVGKLSGAMGTFAHLSPSIEKSVCQRLGLNPAPISNQVLQRDR
                     HAAFLSVLALMAASIEKIATEIRHLQRTEVLEAEEYFEPGQKGSSAMPHKRNPISSEN
                     LCGLARVVRSNSLAAMENVALWHERDISHSSAERIILPDSTILINYMLVRLTNMLSKL
                     VVYPERMMATLNQTGGLIYSQRVLLALLDKGAIRKNAYEHVQQQAMAAWKGAGSFQSL
                     IEGDPFIAKHLSAKEIATCFNPRAYLRNQQRIFRRVFGRHPQSQRSTPTRATHPRKIS
                     KKRKAE"
     gene            complement(3266..3793)
                     /gene="nusB"
                     /locus_tag="PKM72_09435"
     CDS             complement(3266..3793)
                     /gene="nusB"
                     /locus_tag="PKM72_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNP61050.1"
                     /translation="MTMTVPSGTPPANQAQGRPSRRLARQLALQMLFQYEFQDQNPSW
                     QEKFWASQSASPEVQTFTSNLFRGVITNQSEIDEIIRDFAIDWSLQRMPVVDRNILRC
                     SIYELLWEPDIPAMVTINEAVELAKLFADDEARRFVNGLLDHVLRAEPKLAIKRGQIQ
                     QKTPAPEFSDPSPTS"
     gene            complement(3790..4302)
                     /gene="ribH"
                     /locus_tag="PKM72_09440"
     CDS             complement(3790..4302)
                     /gene="ribH"
                     /locus_tag="PKM72_09440"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNP61051.1"
                     /translation="MVNQERRGNEVDCQVAIVVSTFNEVVTSRLLAAAESTLTQLGTP
                     PDHRQVIRVPGAFEIPLIAKTLAQSHRFDVVICLGAVIRGETPHFEYICAETSRGIGQ
                     ASLDTGIPIIFGVLTTDTVDQAMERSGQPERNKGADAARTALEMAVLMKQLSKTSIRQ
                     SGFLRESTIS"
     gene            complement(4402..5631)
                     /locus_tag="PKM72_09445"
     CDS             complement(4402..5631)
                     /locus_tag="PKM72_09445"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNP61052.1"
                     /translation="MKKETASIDKPEFHTIAETLEDIQAGKCIILVDDEDRENEGDLV
                     MAAEKVTPEHINFMAKYGRGLICLALTPERVEELKLPPQANENTAPFGTAFTVSIDAA
                     KNITTGISAADRATTIQLAVDPKAKPSDFARPGHIFPLKAHKGGVLRRAGQTEGSVDL
                     ARLAGLYPAAVICEIMNDDGTMARVPHLMKFAKHHGLKIITVKDLIQYRLHRETFVKQ
                     VATANLPTRFGHFQSVVFENELDTGTHIALVKGEIDNLPTLVRVHSGCLTADVFGSLR
                     CDCREQLHRAMEIIEREGKGVLLYLNQEGRGIGLTNKIRAYHLQDQGSDTVEANLQLG
                     FKADLRDYGIGAQILVNLGLKKIRLITNNPRKIVGIEGYGLEVVERVSIEIVPQESNV
                     HYLRTKKAKLGHLLNNV"
     gene            complement(5673..6896)
                     /locus_tag="PKM72_09450"
     CDS             complement(5673..6896)
                     /locus_tag="PKM72_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP61053.1"
                     /translation="MVKDVFHSLRSGHWPTLLGAWLHFEISFMVWLLIGAMGIAISEE
                     FALSSSQKGLLVGTPLLGGALLRIIVGPLGDRLGAKVVGLGILGLEGIGLLLGWLGGT
                     SFESMLGIGLFLGFAGASFAIALPLASQAYPSAHQGLAMGVAGIGNSGVLLAAFMAPR
                     LAETLGWHQVFGIMLLPVMGTAFIFFWLVQSAPVTNETDSSTIDSIRVLLRKGLQDPV
                     MYWLCFLYGVTFGGFVGLSSYLPIFFHDQFQMDMVQAGTLTAFCALTGSGARPLGGLL
                     ADRFGGLTLLQGIFLIITALCLVSGNLDHFVLTYIIILLMMFCLGFGNGAIFQVVFNR
                     FESIMGTASGFIGAAGGLGGFLLPSGFGWLNEITGTFSSGFFALALVSGLAGISVMIG
                     QRSIWLGKPKFVPDS"
     gene            7136..8176
                     /locus_tag="PKM72_09455"
     CDS             7136..8176
                     /locus_tag="PKM72_09455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HNP61054.1"
                     /translation="MKQKLSKKQSSDVIDVAEKDSSSKLANEEVHEEILLDGAGEPEK
                     QPESEEAPENKETWDSVSTALIPTTTLSRYLAEVRRYPFLSKEEELQLFHEYQTAGTR
                     ESAVKLILANLRVCVSIASEYGLAGIDQMDLIQEGNVGLLQAMKKFDPTKNVRFYAYA
                     AWWVRAFVLRYLLNNFRLVKIGTTQEQRRLFYNLRREKAKLERQGYVPDPKLLADRLN
                     VRERDVVEMDQRLGSWELSLDQPMTAEGEGTFHDLLPSTQPPIDDQLGDSQLRLLFRK
                     KLAEFAKTLSEREEDILRNRLLSESPVTLEDLGRKYMITKERTRQLEAKIIKKLRELM
                     KKDIQDFEHLRK"
     gene            8435..8752
                     /locus_tag="PKM72_09460"
     CDS             8435..8752
                     /locus_tag="PKM72_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNP61055.1"
                     /translation="MAATKEKEKKESKEGSSAKGFRPLGDRVFVAYTEELERTAGGIY
                     VPDSAREKPQRGTIEAAGPDVENVKVGDQVLFDKYSGTKIKVENDDCLILKEEDILGV
                     FEN"
     gene            8828..>9373
                     /locus_tag="PKM72_09465"
     CDS             8828..>9373
                     /locus_tag="PKM72_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNP61056.1"
                     /translation="MAKQLQYSEKARASILSGVNQLADAVKTTLGPKGRNAILDKKFG
                     APTITKDGVTVAKEIELKNPYENMGAQLVKEVASKTSDIAGDGTTTATVLAQAIYREG
                     VKNITAGANPMELKRGIDKAVEVAIEELKKISKPCQTKKEISQIGSISANNDHTIGDL
                     IAEAMDKVGKDGVITVEEAKSM"
BASE COUNT         2625 a         2272 c         2107 g         2369 t
ORIGIN      
        1 tatcatgtag tttatacagt ttggggtgtt ttgtgttttt ccaatgctga aatcattgat
       61 cgaaaaatat acaacccatc ctcgttattg agaaggtgtt ctgcacctcg ctctggatga
      121 ggcatcaaac caagaatgtt gccggccgcg ttacgaattc ccgcaatgct atccaacgat
      181 ccattgggat tagcttgaga agtgactttg ccttcacgat cacaatatcg aaaaacgatt
      241 tgcgcattgg cctgcaaggc ggacaacgtc acagggttcg aataataatt tccttcagca
      301 tgggcaatcg gcaacttcag gatctgtccg ggttcacagt gattggtaaa tggagtattc
      361 gcgttttcta cccgtatata gcggtcttca caaataaaag aaagatcccg gttacgcaac
      421 atggcaccgg gtaatattcc aagctctaaa agcatttgaa atccattgca tatgcccagg
      481 accattcctc cccgcccagc aaaatcaaca accgcgtcca tgatgggaga aaatcgggct
      541 atggcacctg tgcgcagata gtccccataa gaaaagcctc ctggcagaat aatggcatcc
      601 atgcctttga gggatcgctc ttgatgccaa ataaaatgcg cagtttgttt catgatcttc
      661 gaaaggacat atccacaatc acgatcacaa ttggagccgg gaaatacaat gataccgatg
      721 ttcatattga actccccctt gtgtcattac tactgagagg agggagctta gccgggaaaa
      781 aatcgaattt aatcaagaga gacaaccggg agaccatcag ttacgcctca atgatctcgt
      841 attgataatc ttcaatgacg gtattggcaa gcagtttttc gcacatctct tttatactat
      901 ttcctgcaga tgtcctgtct gtttcgcaaa gatccacctc gatgaatttc cctatgcgga
      961 cctctccaac cgtgtcaaaa ccaagagttt gaagcgattg ttgaatggct cgaccttgag
     1021 gatccaggat tccgtttttg agcgtaatgt aaattttggc tttcatgatt tcacagaaga
     1081 cccgccacgc attgggtgaa taaaggcttg acgtcacagc cagggttgtt gccaagatgg
     1141 cttttgccaa agaacgagcg cattactcga aataatgcca attatccgca gactcgtttg
     1201 agaacttctt gataggtttc ctcgatccgt cccagatctt ttcggaaacg atccttatcc
     1261 atgcgttccc cggtttgaat atcccaaaaa cgacacgtgt ccggggaaat ttcatctgcc
     1321 aaaatgatct gaccgttcca caatccgaat tctaacttaa aatccaccag catcatgccc
     1381 cttttctgaa aaaaaggttt taacacttcg ttgatcttga gtgcctgctg tcggagttct
     1441 tcaacttgcg ggggtgtggc caatttaagc aggcgaatat ggtcctcgga aatcaaaggg
     1501 tcgcccagcg aatcgtcttt atagaagaat tcgactaatg ggggatcaat gatcaaacct
     1561 tcttctaacc ccaggcgttt gacaatgctg ccggtggctc gattacgcac cacaacttca
     1621 actggaataa ttttcactcg tcgcgttaac atttcccgat cattgagttg ctggataaaa
     1681 tggctgggag tacccgcttc agcaaggtat tgaaaaagcg tggcggagat tttattgttc
     1741 accacccctt tttccagaat cgtcccccgt ttttgcgcat tgaaggcggt tgcatcatcc
     1801 ttgaagtagt gaatgacttc gtctaatttt tcggtcagga aaattttttt ggcttttcct
     1861 tcgtaaaaca tatctccctt agtcattctg ccttcctctt tttggagatt tttcttggat
     1921 gagtagctcg ggttggtgtt gatcgctggc tttggggatg ccttccaaac acccgtctaa
     1981 aaatccgctg ctggtttcgc aaataggctc tgggattgaa gcaggtcgct atctcttttg
     2041 cactcaagtg tttggcaata aagggatccc cctctataag agattggaaa cttcctgccc
     2101 ctttccaggc agccattgcc tgttgctgca catgctcata ggcgtttttt ctgattgcac
     2161 ctttatctaa caacgccagt aacactcgtt gcgaatagat cagtccaccg gtttgattca
     2221 gagtggccat catccgttca ggataaacca ccagtttgga taacatgttc gttaaccgga
     2281 ccagcatata attgatcaaa atcgtgctat ccggaagaat aatccgctca gcagatgaat
     2341 ggctgatgtc ccgttcatgc cacaaggcaa cattttccat ggcagccaga ctgttacttc
     2401 gcactaccct ggcaagacca cataaatttt ccgagctgat cggattccgt ttatgaggca
     2461 tggcggaaga gcctttttgg cctggctcaa agtattcttc agcttccagg acttcggttc
     2521 tttgcaaatg acgaatttca gtggcaattt tttcaatact cgctgccatc agtgccaaga
     2581 ccgaaaggaa ggccgcgtgc cggtcccgct gaagcacctg atttgaaata ggcgccgggt
     2641 tcaaccctaa ccgttgacaa accgattttt cgattgaggg agaaagatgg gcaaatgtgc
     2701 ccatggcccc ggaaagcttg ccgacagcaa tgtccacgaa cgccgcctct aagcggattt
     2761 gctgccgttg aaactcctga taccaaatgg cgaccttcca tgcaaaggaa ataggctctc
     2821 catggatccc atgtgaacgc cccaccatca gggtatcctg atgcgcaaaa gctaaatccc
     2881 gcaaggcatc aagcaaggct ttgacatcct cacgaattaa ttgtgcggcc tccactaatt
     2941 gcaacgaaag agacgtatcg acgatatccg aagaggtgag cccaacatgc aaaaatcgtc
     3001 cgtcattccc tgcctgctcc gccacggatt ccaggaaggc aataacatca tgttttgtga
     3061 cttgttcgat agccgcgatt cgtaagggat taaccttgac cttttttcga atgcgggctg
     3121 ccacgccagg aggaatctgt ctcatttttt ccatggcctc acacacggca agttcgacct
     3181 ccaaccacaa ctcatatttg tgggtctggg tccaaacggc ccccatacgg ggtaacgtgt
     3241 agcggtcaat catgggaaag gacagttagg aggtaggtga tggatcagag aattccggcg
     3301 ctggggtttt ctgttgaatt tggcctcgct tgatggcaag ctttggttca gcacggagaa
     3361 catggtctaa aagaccattg acgaatcgcc tggcctcatc atccgcaaaa agtttagcca
     3421 attcaaccgc ttcattgatg gtcaccatgg ccggaatatc aggctcccat aacaattcgt
     3481 aaatggaaca tctcaaaata ttccgatcca caaccggcat acgttgcaag gaccagtcga
     3541 tggcaaaatc acgaataatt tcatcaattt cagattgatt cgtaatgacc ccacgaaaga
     3601 gattcgaggt aaatgtttgg acttccggtg atgcagattg acttgcccaa aacttttctt
     3661 gccatgatgg gttttgatct tgaaattcgt attggaaaag catttgcaat gcaagctgac
     3721 gggccaaacg cctggatggc cgaccctgag cctgattagc tggtggtgta cctgatggga
     3781 ccgtcatagt catgatattg tggattctct taaaaagcca gactggcgga tgctggtctt
     3841 actcaactgc ttcattaaaa cggccatttc caaagccgtg cgggcggcat ccgccccttt
     3901 attccgttca ggttgaccgg aacgttccat tgcctgatca acggtatcag tagtcagtac
     3961 tccgaaaata ataggaattc cagtatccag ggatgcttgg ccgatcccgc gacttgtctc
     4021 tgcacaaata tattcgaagt gtggcgtttc cccgcgaatc actgccccta aacagataac
     4081 aacatcaaat cgatgagatt gggccagtgt ttttgcaatt aatggaatct caaatgcccc
     4141 aggaactcga atgacctgcc tgtggtctgg tggggttcct aactgggtta aggtggattc
     4201 agctgcagcc aataagcggc tggtcacgac ctcgttaaac gtactaacca cgatcgctac
     4261 ctgacaatct acctcatttc cacgacgctc ctgattcacc atgctagtta agtgtttcct
     4321 taagactcaa tccctgatta ttaaaagtga gagaggggcg atcagtgtgt accacttgcc
     4381 agtgggaacc gcaagtcccc attacacatt attcaagagg tgacctaatt tggctttttt
     4441 cgtgcgtaag taatgcacat ttgactcctg gggcacaatc tcaatggaca cgcgttccac
     4501 gacttccaag ccataaccct cgatccccac aattttcctt ggattattgg tgatgagtct
     4561 gatttttttg agacctaaat ttactaaaat ttgggcgcca attccataat cccgcaaatc
     4621 agctttaaac cccagttgca aattggcttc cactgtatca cttccctggt cctgaaggtg
     4681 gtaagcccga attttattgg tgagtccgat tccccgcccc tcctgattta agtacagcaa
     4741 aacccctttg ccttcccgtt caattatttc cattgcccta tggagttgtt cacggcaatc
     4801 acatcgcaaa gatccaaaaa catcggctgt caggcagccc gaatggactc gcactaaggt
     4861 tggaaggtta tcgatctccc cttttacgag ggcgatgtga gttccggtat caagttcgtt
     4921 ttcaaaaacc accgactgaa aatgaccaaa gcgtgtaggg agatttgctg tggcgacctg
     4981 ttttacaaac gtctcccgat gtaaccgata ttggatcaaa tctttcactg tgatgatttt
     5041 caggccatgg tgctttgcaa atttcattaa atgaggaacc cgagccatcg tgccgtcatc
     5101 attcataatt tcacagatga ctgctgcggg atacagtcca gccagacggg caagatcaac
     5161 agacccttcg gtttgtccgg ctcggcgtaa tactccgcct ttatgagctt tcaatggaaa
     5221 aatgtgccca ggccttgcaa agtcgcttgg cttggctttt gggtcaacgg ccaactgaat
     5281 ggtggtggct cgatcagcgg ccgagattcc ggtggtaata tttttggcgg catcaatcga
     5341 tacggtaaaa gccgtgccga aaggtgcggt attttcattc gcctgggggg gaagcttcaa
     5401 ctcctcaact cgctctgggg tgagggccag acaaattaac ccacggccgt attttgccat
     5461 aaaattaata tgttcaggcg tgactttttc agcagccata accaggtcgc cctcattttc
     5521 ccgatcctca tcgtcaacaa ggatgatgca tttaccagct tggatatcct ctaaggtttc
     5581 agcaatggtg tgaaattcag gcttgtctat tgaggctgtt tcttttttca tgggtgactc
     5641 acttgacggt caaacgtttc aacttcagct tgttaactgt ctggaacgaa ttttggcttg
     5701 cctaaccaga tcgaacgttg gcctatcata acactaatgc ctgctaatcc tgaaacaagg
     5761 gccagggcga aaaatccaga agagaaggtt ccggtaattt catttaacca tccaaaccca
     5821 gatggtaaga ggaatcctcc aagacctcct gccgcaccaa taaagccgga agccgtcccc
     5881 attatggatt caaacctatt gaaaaccacc tgaaaaatgg ctccattacc aaatcctaaa
     5941 caaaacatca ttaggagaat aatgatatag gtcaagacaa aatgatcaag attcccggag
     6001 acaaggcata gggcggttat tataagaaaa atcccttgca ataaggttaa ccccccgaac
     6061 cgatcagcaa gcaatcctcc cagagggcgg gcaccgcttc ccgttaaagc gcaaaaggcg
     6121 gtcaatgttc cagcttgtac catatccatc tgaaattgat catgaaaaaa aattgggaga
     6181 taactcgaaa gccctacaaa ccctccgaac gtcaccccat ataaaaaaca caaccagtac
     6241 atgaccggat cctgaagtcc ttttcgcaat agtacacgga tgctgtctat tgtcgaggag
     6301 tcggtttcat tcgttaccgg agccgattga accaaccaga aaaagatgaa agctgtcccc
     6361 ataacaggta ggagcataat gccgaaaacc tgatgccaac caagtgtttc cgcaaggcgc
     6421 ggggccataa acgcagctaa tagcacgccg ctatttccaa tacctgcgac tcccatggcc
     6481 aacccttgat gcgctgaggg ataggcttgg ctggccagag gcagggcaat cgcaaaactg
     6541 gctccggcaa atcctaaaaa aaggccaatt cccagcatag actcgaagct ggttcctccc
     6601 agccacccca gcaacaaccc gatgccttcc aagcccagaa tgcccaatcc cacgactttg
     6661 gcccctaacc ggtctcctag cggtccgaca ataattcgaa gcaatgcccc ccctagcaat
     6721 ggggtcccga ctaataagcc tttttgagag gacgagagag caaattcctc agatatggcg
     6781 atgcccatgg ccccaataag gagccacacc ataaaactta tttcgaagtg gagccacgca
     6841 ccaaggagag ttggccaatg cccggaacgg agtgaatgga aaacgtcttt caccatgatt
     6901 ctgtcaagag catggaaagt cctttctttt ttcatttttc aagcctgcag tcatgaccca
     6961 actataaaaa gtttcaccag acattgccaa tatttttctg aaatgaaact tctgaaagcc
     7021 tggttattac cttgaaatta gaaattttcg cgaatgtgtt tggtgatcca agaaaaattc
     7081 attacaataa caatagaatg tagcgtccct gccttcttga tcagtaagcc aaagcatgaa
     7141 acagaaatta tcaaaaaaac agtcttccga tgtgatcgac gttgcagaga aagactcttc
     7201 atctaagctg gcgaacgaag aggtccatga agaaatactc ctcgatggag ccggggaacc
     7261 ggagaaacaa ccggaaagtg aggaggcacc tgagaataag gagacctggg attcggtctc
     7321 aaccgcactt attccgacga ccacgctcag tcgttattta gccgaagtcc gccggtatcc
     7381 attcctttcg aaagaggaag agcttcaact ctttcatgaa tatcaaacag cagggacacg
     7441 agaatctgca gtgaaattaa ttttagctaa cctgcgtgtg tgtgtgtcca ttgcctcaga
     7501 atatggtctt gccggaattg atcaaatgga cctaatccag gaagggaatg tcgggctttt
     7561 gcaggccatg aagaaattcg atcctacaaa aaatgttcgc ttttatgcct atgccgcctg
     7621 gtgggttcga gcctttgtac tgcgatatct cctcaataat tttcgtcttg tcaaaatagg
     7681 cacgacccag gaacaacgac gccttttcta taatcttcgt cgtgaaaagg ccaaattgga
     7741 gcgacaaggc tatgtccccg accctaaatt actggctgac cgccttaatg tgcgtgaacg
     7801 agatgttgtg gaaatggatc aacgcctggg aagttgggaa ctttccttgg atcagccaat
     7861 gactgctgag ggggaaggca catttcatga cctccttccc tctactcaac ctcccattga
     7921 cgatcagttg ggtgactcac agctgcgact cctcttccga aaaaaattgg cagaatttgc
     7981 caaaaccctt tcggagcgag aggaggacat acttcgcaac cgactccttt cggaatcccc
     8041 tgtgacctta gaggacctgg gaagaaaata tatgattacc aaagaacgga cccgtcaact
     8101 tgaggccaag ataattaaga agttgcgaga acttatgaaa aaagacattc aggattttga
     8161 acatctccgg aaatagggtt ttaagcttat atacgtatgg aaatccgcat gccagcagat
     8221 cgtgaatgta tgctttccct aaagatagca tgcccgacac tcttccatgt tgaaaatctt
     8281 gcctttccct cttgacttca aatcaagcgg acttatctct cctttcaaga tgccgcatct
     8341 ataaaaaatt caggcaggtg tgcgtaatcc caattgatta cagttctcac catttttcca
     8401 tttaatgggc aacaaacaaa aaaaggaggc agacatggca gccacaaaag agaaggaaaa
     8461 gaaggaatca aaagagggaa gcagtgcgaa gggattccgt cctttaggag accgagtgtt
     8521 cgtggcctac actgaagaat tggaacggac cgcaggggga atttacgtac cagactcggc
     8581 tcgtgagaaa ccccaaagag gcactatcga agccgcaggc cctgacgtag aaaatgtgaa
     8641 ggttggcgat caagttttgt ttgacaagta ttctggaacc aaaatcaagg ttgagaatga
     8701 tgattgttta atcttaaaag aggaagatat cttgggcgta tttgaaaatt aacgccgccg
     8761 tcacgttcaa acccagtttt ttgagttgta ttccacacca caaaaatgat ttctaaggag
     8821 gatttgtatg gctaagcagt tgcaatacag tgaaaaggca cgtgcgtcta ttttgagcgg
     8881 agtgaaccaa ttagccgatg ccgtaaaaac gacattggga ccgaaaggcc ggaatgccat
     8941 tctcgataag aagtttggcg ccccaacaat taccaaagac ggcgtaacgg tggccaagga
     9001 aatcgaatta aaaaatccct atgaaaacat gggggctcaa ttggttaagg aagtggcaag
     9061 caaaaccagt gatatcgcag gtgacggaac aaccaccgcg accgtattag cacaagccat
     9121 ttaccgtgaa ggcgtcaaga atatcaccgc aggcgccaat cccatggaac ttaagcgagg
     9181 cattgacaag gccgttgaag tcgcaattga agaactaaaa aagatcagca agccctgcca
     9241 gaccaaaaaa gaaatttccc aaatcggttc aatctccgcg aataatgatc acaccattgg
     9301 tgacctgatc gccgaagcaa tggataaagt gggaaaagat ggcgtcatca ccgtagaaga
     9361 agccaaatcc atg
//
LOCUS       DAORAQ010000101         9282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1181_length_9282_cov_65.562371, whole genome shotgun sequence.
ACCESSION   DAORAQ010000101 DAORAQ010000000
VERSION     DAORAQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9282
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1181_length_9282_cov_65.562371"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..948)
                     /locus_tag="PKM72_09470"
     CDS             complement(610..948)
                     /locus_tag="PKM72_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP61057.1"
                     /translation="MIIKERTMNGLPVVSLSGKLDIFSKNAFKETAEKYTDAKSKGLI
                     LDFQGVTFLDSVGFGALVVLARTFLKLKGRIIIVNPQDQVKTLLCEMHMEKMIPMYAT
                     DDQFSTFSEL"
     gene            complement(1205..2218)
                     /locus_tag="PKM72_09475"
     CDS             complement(1205..2218)
                     /locus_tag="PKM72_09475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HNP61058.1"
                     /translation="MSDARSIFIGFVFFVGLGFLPGLGEVRAMPISHAIEQAKLATVG
                     ILPADQPEISDNDYGLPVSIRGSGIHIGGGVIVTARHAVERTEGGKVIIPDTIHVVTD
                     DLFELPATRQGANAYLDVAVYQLQGPESDWPQAHVTFADEDVTYGDQVFTVGYPLGWG
                     PAINFGTTGNPNTFLPTVNSRLMQVDMSACSGNSGGGLLNQHGQLVGLMHAIIQTDTQ
                     HEDRRCARLAFVLPGPLVQRVVTAVLDGRVPGFSVLGIQLQTIRTGSRWALVVAKVTG
                     PSRHAGFQKGDVLVAINDVPITTPAQLKNYLIERTEPGQVVEIKIQRGDAQKLLSVTL
                     GRS"
     gene            complement(2412..3014)
                     /locus_tag="PKM72_09480"
     CDS             complement(2412..3014)
                     /locus_tag="PKM72_09480"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HNP61059.1"
                     /translation="MKTRVGGSKHRGRMPTSYEHSGLPDYLAPGLSILFVGINPGLRS
                     AEVGHHYAGPSNRFWKLLFDSGLIPSPLTYRDDWQLPEFGYGLTNMIARPTAGVQDLG
                     KQDFLKGHQALLTKIMTYQPRLVALLGVSMARMLLSSNEATSVNQRTLGNPIQVGLQS
                     IPLCGVPVWVLPNPSGRNAHYSYQQMKALYCGLKDWGHPS"
     gene            complement(3179..3829)
                     /locus_tag="PKM72_09485"
     CDS             complement(3179..3829)
                     /locus_tag="PKM72_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61060.1"
                     /translation="MTLTHRMLYILFASVLLSWLIIPGVWGEESSRILTQQAMDACHE
                     GRRAKDETARVAHFERGRELAKKSVELDENSADAHFALFCNIGERMRANGEVLFSVFE
                     YGRMMEALDKTLLLNPNHMDALSSKGTILIEVPGLFGGDSEKGEAMLREVIQKDPEAI
                     NARMVVARLCVERGEDQEAFNLAKRALELARAANRDDLLPDAEKTFSEIQTKLASK"
     gene            complement(4062..5315)
                     /locus_tag="PKM72_09490"
     CDS             complement(4062..5315)
                     /locus_tag="PKM72_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNP61061.1"
                     /translation="MPLKSDNMKIAVIGGGPAGLMAAEAAIAMGARVDLYDAMASVGR
                     KFLLAGKGGLNLTHSEPLGNFILRYGPRQEQIRSLLAGFGPDALREWARELGVETFIG
                     TSGRVFPTDLKSAPLLRSWLRRLRQHGMTIHVRHRWCGWAGSETLRFSTPQGDRLVSP
                     DAVVLALGGGSWPRFGSDGAWVPLLAGRGVLIEPLKPTNCGFDVEWSPHLRGRYAGHP
                     VKPVVVAGTTQEGVEFRQQGEFVLTETGIEGGVIYAISAWLRDEIVKRGTVLLRLDLA
                     PDRDVSRLISDLSRPRGSRSMASHLQRRVGISGVKAGLLREYVSKKDFADPILLGTAI
                     KSLPLRLVAPRPLEEAISTAGGVAFEALDGRLMIRTLPGVFCSGEMLDWEAPTGGYLL
                     TACFASGHAAGVGAVRWFQSTRRST"
     gene            5565..5732
                     /locus_tag="PKM72_09495"
     CDS             5565..5732
                     /locus_tag="PKM72_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61062.1"
                     /translation="MEQEEIRQLWADGEDWIIKRQHNQYFHRPDGKYGDWKSGLPPGV
                     LKPDVDTLFDD"
     gene            5874..6227
                     /locus_tag="PKM72_09500"
     CDS             5874..6227
                     /locus_tag="PKM72_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61063.1"
                     /translation="MHDLVEQAKQEEAAHASFGFASHPYRADEAISDLLAILDDRIES
                     EGVQVGLPIEFLHQMWTLCNQATTHISETVWLRKNLDQGTGSKAEVRQLTYQALIAFL
                     ESQPEEDESSGATPN"
     gene            complement(6185..7057)
                     /locus_tag="PKM72_09505"
     CDS             complement(6185..7057)
                     /locus_tag="PKM72_09505"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNP61064.1"
                     /translation="MDATVFEMTVPVSVDASELAGILDCQEFLGAWEAEGSVVLYWSK
                     NGAAILQQVRSAISALGVVVPPEGSLQFQPVKAQDWNATWAASVQPIRIGRRIGIRPS
                     WATMDMPKDGVELIIDPKQAFGTGHHATTQLILEWLEGVTWVPGMRVLDVGTGSGILA
                     MAALRLGATTALGIDVDTTALDCAREYAAVNNIQQELTLSSCQLATLPDQSFDVILAN
                     LDRKTILQVMGQFSRLRGLHTQLVLSGLLEEDEAEIVGQLEGQGWLRRHVRRWDGWIA
                     IQLGVAPLDSSSSG"
     gene            7288..9165
                     /locus_tag="PKM72_09510"
     CDS             7288..9165
                     /locus_tag="PKM72_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61065.1"
                     /translation="MERISTDRREQAILRLIHRTERMQAKGAIASQQFSRWRMGIFLA
                     GAAITIGVYQQAWFHTGNGLLLIFLIGFLTISWFHQRLKSRLHRLRLWLEIKQDSFAR
                     LQLDWPHISADEHKAPERHPFALDLDLTGPHSLLGLIDTTFSSIGQKRIATWLFQSNL
                     PESNGLSWTTRQTLVKELTPLSRLRDRCLLATRLISSERLNGTRIISLLEAPMAIRHL
                     SLIIIAACALTSLTIALGLWTLLTGAPNFWLLPLTLYIGTYFLLSGSIHPLFGRVLAL
                     HEELEKLVSVIATLERSTFSRQPTIRQVCQSILTKQHRPTQSLKHLSRICQGLSVKAH
                     PLIHLGLNALIPWDLLFVGKLNRLITRLRASLPDWLEIIGTLDAASALARYAYLNPDY
                     LWPHLEATNVTPDTRGLTTCGLGHPLIPRAHRITNDLELRGEGHLLLITGSNMSGKST
                     FLRTVGINLCLAQAGAPVCAGIFSWTWLRLYCCIRVTDALDEGLSYFYAEVKRLKVIL
                     EAVKSPNPDPVLFLIDEIYRGTNNRERLAGSEAFVQALQQSRGLGMISTHDLELTGLA
                     NGNGHIRNAHFQETVEAGTLHFDYQLRPGPCPTTNALRIMAQEGLPVPSDHSSNPHKE
                     C"
BASE COUNT         2398 a         2729 c         2111 g         2044 t
ORIGIN      
        1 tggtcccata ggctttggta agcttctgtc cataaatcag cgctgacata cgtattacac
       61 tcctttaaat tccgaccttc ataaaccctg aaattaataa tgatcttttc ccacctgtgg
      121 aactcatcca ttcctgccgg acaggactca ccagagacgg accccgctca acctatttgc
      181 cttcagagac aggccacccc tgcgggtcga agcccccgca gaccccatac catcgacaat
      241 cgcatcgtat tgaaaggaat gaagagggag agtcaccggt cagaggtaaa ttttcgtaaa
      301 aaatggattt gcgagacaaa cctgaacagg ttggagagta ggaagctttc cggtaacgaa
      361 aaccgtatac actttcacca atttttcact actgtactgc aattctctct aggttgacta
      421 ccccgtcatc atactcaaaa gaaaacacag acacatacag tggagcattt ttgtcccggg
      481 atggagaatt atccccccac cattgaaggg atccagtaga aacgggtgga tgatacgtaa
      541 gagaatctca gaagactcat caccaaaaaa aacacgtgat aatcgtgaag gaagaaggag
      601 gcaggggagc taaagttcag aaaacgtaga aaattggtca tccgttgcat acatgggaat
      661 catcttctcc atatgcattt cacataataa ggtcttcacc tgatcctgag gattcacgat
      721 aattatcctc cccttgagtt tcaggaatgt cctggcgagg accaccaaag cgccgaaccc
      781 cacactatcc aaaaaagtta ccccttgaaa atccaggata agcccttttg acttcgcatc
      841 tgtatatttc tctgcggtct ccttgaaggc atttttagaa aaaatatcca actttcctga
      901 aagactcacc acaggcaagc cattcattgt tcgttcttta ataatcatag cccggcattg
      961 atccttcttt ctccaaggcc cgatccacag gagattaccc catgtttcag tgcgtttggt
     1021 gggtctatcg gaccatttgt agggaatctt gaggattatg gcctgattca tgacctttca
     1081 ccggcgtgac tcgattcctt tagcctattt ttccatactc ccaagtaagg gaaggcctgg
     1141 catgggaaac atggaaatcc acaaggccgg aaagcctctt gaacaaagac aaacaaaggc
     1201 attcttagga tcgacctaac gtaaccgaga gcagcttctg agcatctcca cgctgaatct
     1261 tgatctcgac aacctgaccg ggttcggtac gttcaatcag ataatttttc aattgtgccg
     1321 gtgtggtaat aggcacatca ttaatggcca ccagcacatc gcctttttga aatcccgcat
     1381 gacgtgaggg accggtcacc ttggccacga ccaacgccca acgacttccc gttcttatgg
     1441 tttggagctg aatccccagc accgaaaacc ccggcaccct cccatccaag acggcggtca
     1501 ccactcgttg aaccaacggg cccgggagaa caaaagccaa tcgggcacag cgccgatctt
     1561 catgttgcgt atccgtttga atgatggcat gcatgagacc caccagctgt ccatgttgat
     1621 tcaataaccc gccgcccgaa ttgccgctac aggcggacat atccacctgc attaaccgac
     1681 tgttcaccgt cggcaaaaag gtattgggat tgccagtcgt gccaaagtta atggccggcc
     1741 cccaacccag tggatatccg acggtaaaga cctgatcgcc atacgtcaca tcttcgtcgg
     1801 caaacgtgac atgcgcttgg ggccaatccg attcaggacc ctggagttga tacaccgcca
     1861 catccaggta agcattcgct ccctgccggg ttgccggcaa ctcaaacaga tcgtccgtga
     1921 ctacatgaat ggtgtcgggg ataatgactt tgcctccctc tgtacgctct accgcatgcc
     1981 gcgccgttac aatcactcct ccgccaatat ggattcccga tccccgaata ctgacgggca
     2041 gtccatagtc attatcagaa atctcaggtt gatcagccgg gagaatcccg actgtcgcca
     2101 acttggcctg ctcaatggca tgggaaatcg gcattgcccg aacctctccc aaccccggca
     2161 aaaagccaag gcctacaaaa aaaacgaatc caatgaaaat cgatctcgca tcactcatcc
     2221 ataccccctg cgtttcagaa tggcccccaa agcgagaaac acgcctcctc attcgaacca
     2281 actcactctc gcggatattc acaaagaaca cagatcacgt aagattcgtc tatacatacc
     2341 tcaaacacaa gatcaaagca ttagtggttg ccagatgcga gcggctctaa tgggtgggct
     2401 actccctctc ttcaggatgg atgaccccaa tccttcagcc cacagtacaa agctttcatt
     2461 tgctgatagg aataatgggc attccttcca ctcggatttg gcaacaccca gacaggcaca
     2521 ccacaaaggg gaatagattg caggcccacc tgaattggat ttccaagcgt ccgctgatta
     2581 acactggttg cctcattgga cgagagaagc atgcgtgcca tgctgactcc taacaaggcc
     2641 acaagtcgag gttgatatgt catgattttt gtcagtaatg cctgatgccc tttgagaaaa
     2701 tcctgttttc ccaaatcctg aacaccggcg gtcggcctgg caatcatatt ggtaagccca
     2761 tacccgaatt caggcaattg ccaatcatcc cgataggtca ggggagaggg aatcagcccg
     2821 gagtcaaaca gcagcttcca aaaccggttg gaaggtccgg cataatgatg accgacctca
     2881 gccgagcgca gaccagggtt aattccaaca aagagaatgg ataacccggg ggccagataa
     2941 tcgggtaacc ctgagtgctc ataacttgtg ggcatacgtc cccggtgctt ggatccaccc
     3001 acgcgcgtct tcatacatta attatagagc acgggctttt gcagtattgc ctaagaaccc
     3061 agccaccaca aggtaggaaa cgtttgaaaa ataggaaggg attgtggaga aaaacaggaa
     3121 aaagctagaa gaaaaattcc attccaattc gggcacctgg caaaaaccaa atcgcgattt
     3181 atttggaagc cagtttcgtt tgaatctcgg aaaaggtttt ttccgcatca gggagaagat
     3241 catcccggtt tgccgccctg gcaagctcca acgccctttt cgctaaatta aacgcctctt
     3301 gatcctctcc ccgctcaaca cataatcgcg cgacaaccat gcgagcatta atggcttcag
     3361 gatccttctg gatcacctcc cgcagcatcg cttcgccctt ttcggaatct cctccaaata
     3421 acccggggac ttcgatgagg atcgtccctt tagaagagag ggcatccata tggttaggat
     3481 tgagtaaaag ggtcttgtcc agagcttcca tcatccgtcc atattcaaac accgagaaaa
     3541 ggacttctcc gttggcccgc atacgctcgc caatattaca aaacagggca aaatgcgcgt
     3601 cggcggaatt ctcgtcgagt tccacagact ttttagccag ttctctcccg cgttcaaagt
     3661 gagcaacccg agccgtttcg tcctttgccc gtctcccttc atgacatgca tccatcgcct
     3721 gttgcgtcaa aatccgagag gattcctcac cccagactcc aggaataatc aaccaggaaa
     3781 gcagcacact ggcgaacaaa atataaagca tgcgatgagt cagggtcata ttgtacaagt
     3841 cctttgtgca ctactcggat gaattccagc caacaaaagg ggggacagga cacaaagtaa
     3901 atattttata ccaagtgaaa tgctcatgcc aacgcctggg gacgtaaaat gttctcaccc
     3961 ggttttcctt ggggaattaa cggtcactgt tgggatgaaa aatgaggaag gggtccatga
     4021 ccagagctca tgcggggggt cgccggttgt cagaactttg cctaagtgga acggcgggtt
     4081 gactgaaacc acctcacagc tccgacaccc gcagcatgac cactagcaaa acaggcagtc
     4141 aaaagatagc cgccggttgg cgcttcccaa tccagcatct ctcccgaaca aaatacaccc
     4201 ggtaaggtgc gaatcatcag tcgcccgtcc aaggcttcaa acgctactcc acccgccgtg
     4261 ctgatcgctt cctccagcgg gcgcggcgca acgagacgta acggcagcga tttgatggcc
     4321 gtcccaagta ggatggggtc ggcaaagtct ttcttggaca catattccct cagaaggccg
     4381 gctttcaccc cggaaatacc aacccggcgc tgcaggtgac tcgccatcga gcgtgaccct
     4441 cgcggccgtg ataagtctga aataaggcgc gacacatccc ggtccggcgc cagatccaat
     4501 cgaagaagca ccgtgcccct tttcacaatc tcgtcacgga gccatgccga aattgcatag
     4561 atcacacctc cttcaatgcc ggtctccgtc agaacgaatt ccccctgttg cctaaactca
     4621 acgccctcct gggtggttcc cgccacgaca accggcttca cgggatgccc ggcataacgg
     4681 ccacgaaggt gaggactcca ttcgacatca aaaccacaat tcgtcggctt cagcggttca
     4741 atgagaacac cacgcccggc aagcaacggt acccatgccc catcggagcc gaatctgggc
     4801 caactgccac cacccaacgc gagcaccaca gcatcaggag aaacaagacg atctccttgc
     4861 ggcgtggaaa accggagagt ctcgctcccc gcccagccac accagcgatg ccgcacgtga
     4921 atcgtcattc catgctggcg caatcggcgt aaccaagacc gcaaaagagg tgcagactta
     4981 aggtcagtag ggaacacgcg gccggatgtt ccaataaacg tctccacacc caattcacga
     5041 gcccactcgc gcaaggcatc cggcccaaat ccggcaagca acgatcggat ctgttcctgc
     5101 cgaggaccat atcgcaaaat aaaattccct aatggctcag aatgcgtcaa gttgagccca
     5161 cccttccctg ctaacaaaaa ttttcgaccg acggaggcca tggcgtcata tagatccacg
     5221 cgtgcgccca tggcaatcgc ggcctccgca gccatcaggc ccgcaggccc cccgccgatc
     5281 accgcaatct tcatattatc tgacttcaac ggcatggaag acccacagga agtgaaggta
     5341 aaaaagcgtg atcacaagac aaatatgcca atcgtctctg agtaggtatt tcggattctt
     5401 tcctgatcca taggctcctc acgaagggaa ttcatcacac caattccctt cacaagtaat
     5461 gtgatttgcc cgaagagaag ggtttcatta agatacttca agcccataca tcacgtatta
     5521 gatctccccc tggtagactt cacaagacag gagaattgaa ttcaatggag caggaagaaa
     5581 ttcgacaatt gtgggccgac ggtgaggatt ggatcatcaa gcgccaacat aatcaatatt
     5641 tccatcgtcc ggacggaaaa tacggagact ggaaatccgg gctccctccc ggcgtgctaa
     5701 aaccggacgt cgacaccctc ttcgatgatt agatggtggg gtcttcctca tctcaaatgg
     5761 aaaattacaa cgtcaaaaaa aaattcattt catctcacga attgttccag agagagcccc
     5821 tcacgacccc catgcgattt tccggacgaa tcattggttt gctcaaggag tacatgcatg
     5881 acttagtcga gcaggcaaaa caggaggaag ccgcccacgc cagcttcggc ttcgcttccc
     5941 acccctatcg ggcagacgag gccatctctg atctcctggc tattttggat gaccggattg
     6001 aatcagaagg cgtccaggtt gggcttccca tcgaatttct tcaccagatg tggactctgt
     6061 gcaaccaagc caccactcac atttcggaaa ccgtctggtt gcggaaaaac ctggatcagg
     6121 gcactggatc aaaagcggaa gtccggcaac tcacctacca agccctcatt gcattcctgg
     6181 agtctcaacc cgaagaagac gagtcgagcg gagcgactcc caactgaatg gctatccagc
     6241 catcccacct cctgacatgg cgtcgaagcc atccctgtcc ttccaactgt ccgacaatct
     6301 cagcttcatc ttcctccagc aacccggaca acaccaattg cgtatgcaaa ccccgcaacc
     6361 tggaaaattg acccattact tgaagtattg ttttgcgatc caggttggcc agaatgacat
     6421 caaatgattg atcgggtagc gtggctaatt ggcaggagga cagtgtcaat tcttgttgaa
     6481 tgttattcac ggctgcgtat tccctggcac agtccagcgc cgtggtatcc acatcaattc
     6541 ccagcgccgt ggtcgctccc aggcgtaatg ccgccatcgc gagaatgcca ctcccggtcc
     6601 cgacatccag cacccgcatg cccggaaccc acgtgacccc ttccaaccat tccagaatca
     6661 attgggtagt agcatgatga ccggtcccaa acgcctgctt gggatcaatg atcaattcca
     6721 ctccatcctt gggcatgtcc atggtggccc aactcgggcg gatcccaatt cgacggccga
     6781 tacgaatagg ctgaacggac gccgcccagg tggcattcca atcctgggct ttcacgggct
     6841 gaaactgcag tgacccttcc gggggaacaa caacacccaa tgcgctgatc gccgaccgaa
     6901 cctgctgaag aatagctgct ccattcttac tccaatagag cacgaccgaa ccctccgcct
     6961 cccatgcccc cagaaattcc tggcaatcca gaatccccgc caactcagaa gcatccaccg
     7021 aaaccggaac cgtcatttca aagaccgtcg catccataca agcttttcca ttatttaaac
     7081 gtttttcaat tgtacgctac tgctgccact catttctcag gggaagaaaa caggaggccc
     7141 tcttcaaata agcgcaaact gcgtggagag caaggagtgc gttgaggact ctgtgtcatc
     7201 aaaatccgac cgaccgcatg cttcaacaag ctcttcgcca atttctattg acgggatcat
     7261 gcagagcggc taatgtccgc aggccccatg gaacgtatct ctaccgatag acgtgaacaa
     7321 gcgatccttc gcctcattca ccgcaccgag cggatgcagg cgaaaggcgc catcgccagt
     7381 caacagttca gccgctggag aatgggaata ttccttgctg gcgccgcgat cacgatcggg
     7441 gtctatcaac aggcgtggtt tcataccgga aatggactac ttcttatctt cctcatcggg
     7501 tttctcacta tatcctggtt tcaccagcga ttgaaatccc ggttgcatcg attacggctc
     7561 tggttggaaa tcaaacaaga cagcttcgct cgcttgcaac ttgattggcc tcatatatcg
     7621 gccgacgaac acaaggcccc tgagcgacat ccgttcgcct tagacctcga cctcacgggt
     7681 ccacattccc tgctgggcct gatcgacacc accttttcct ccattggtca aaagcgcatc
     7741 gccacctggc tctttcaatc caatcttccc gaatcgaacg ggctatcatg gacaaccagg
     7801 cagaccttgg tcaaagaact caccccgctc tcccgtttga gggaccgttg cctcttagcc
     7861 acccgcctca ttagctccga acggctgaat ggaactcgga ttatttccct gcttgaagcg
     7921 cccatggcca tcaggcatct atctctgatt atcatcgccg catgcgccct gacgagtctc
     7981 accattgcct tagggctctg gacgctcctc acgggagcac caaatttttg gttgcttccc
     8041 ctcaccctct atatcggaac gtattttctc ctttcgggat ccattcatcc gctgtttggc
     8101 cgagtgcttg ccctccatga agagctggaa aaacttgtca gcgtgattgc cacacttgaa
     8161 cgatcaacat tctcccgtca accgaccatc cgacaggtct gccagtccat tttgaccaaa
     8221 cagcatcgtc caacccagag cctcaaacac ctcagccgca tctgccaggg gctgagcgtc
     8281 aaagcccacc cgctgatcca tttaggcctt aatgccctga taccctggga cctgttgttt
     8341 gtcggaaaac tcaatcgcct catcacgcgc cttcgcgcat cacttcctga ctggctggaa
     8401 atcattggca ctcttgatgc cgccagcgcc ctcgcgcggt atgcctatct caatccggat
     8461 tatctctggc cacatcttga ggcaacgaat gtcactccgg acacacgagg cctcaccaca
     8521 tgcggcctcg gccatccgct tattccacgt gctcatcgca ttaccaatga tctggagttg
     8581 cgaggggaag gccatctcct tctgatcacc ggttcgaaca tgtcggggaa aagcacgttt
     8641 ctgcgaacag tgggtattaa cctgtgcctg gcccaggcag gagccccggt atgtgccgga
     8701 atattttcct ggacctggtt gcgtctttat tgttgcatac gagtcacgga tgcccttgac
     8761 gaagggttgt cgtatttcta cgcggaggtc aaacgcttga aagtcattct ggaagctgtg
     8821 aaaagcccca atcctgatcc tgttctcttt ctcattgatg aaatctatcg gggcaccaat
     8881 aatcgtgaac ggctggccgg aagcgaagcc tttgtgcagg cccttcaaca aagccgtgga
     8941 ctgggtatga tttcaactca cgatttggaa ctcacaggat tggcgaatgg aaacgggcat
     9001 atccggaatg cccactttca ggaaaccgtc gaagcaggca cgttgcattt cgattatcaa
     9061 ctacgaccgg gcccctgccc caccaccaat gcgctccgta tcatggccca ggaaggcctt
     9121 cccgtcccat ccgatcactc ctcgaatcca cataaggagt gttgaaaaag gccgccagcc
     9181 gcgttctcac cccttggccg tgctcacgta ctcatccgta cgctccgctc gtccaagcgg
     9241 ctgcggcctt tccttcgaga agcctcagga caggactgga cg
//
LOCUS       DAORAQ010000102         9275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1182_length_9275_cov_59.873210, whole genome shotgun sequence.
ACCESSION   DAORAQ010000102 DAORAQ010000000
VERSION     DAORAQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_9275_cov_59.873210"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            569..1264
                     /locus_tag="PKM72_09515"
     CDS             569..1264
                     /locus_tag="PKM72_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61066.1"
                     /translation="MIPQKERASGLAFLPIFATIGFYLLPAHWQLHTAVQFAPQLMAY
                     GALSTWAFNNGDQLHKLGLGFNNIQAGILRGTVIGITLGILNTGVILTVMPAWGVDIN
                     FLSQTPHAQVPFWIMLPWFIILIAMGVELNFRGFLLGRLLIWFQDVRQLDHPSTTNIC
                     LQTFLPLSISALTFSFDPFMVSTFRDLHWIAIWDGLVWGWLWMRWHNLYMVIAAHAVE
                     VILLYLIIRGILT"
     gene            1261..2271
                     /locus_tag="PKM72_09520"
     CDS             1261..2271
                     /locus_tag="PKM72_09520"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61067.1"
                     /translation="MTPSFSPHLVNHPFGDPGLYVDIRWSRRALLFDLGENVSLSPTQ
                     LLRAQDIFISHTHMDHFIGFDRLIRVALGRGKHLRLHGPPGLIENIQGKLRGYTWNLV
                     DGYPLTIEAREYHPTHIQSGHFHAKDAFVCKQENLIPGDHSHIFPVLQEPALLVKAVA
                     LNHRIPSLAFSLEEPFHINIKKARLHEAGLPVGSWLKDAKQLIVDGAPDSHTFSATLY
                     FEHRKEERVFDVGIMKREFVTITKGQKIAYVVDCRYDADNEQRIIQLSQGATTLFCEA
                     PFLEADRDKAANRYHLTARQAGILGRKAGVQQLVVFHFSPRYTGQGDKLHQEAMEAFH
                     EQ"
     gene            2296..2634
                     /locus_tag="PKM72_09525"
     CDS             2296..2634
                     /locus_tag="PKM72_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HNP61068.1"
                     /translation="MPEWVRYGLYFVLGGTLVSVSTYLGSHGRGFLAALASTLPVISG
                     VTFILIFLNAGPAPTVSFAKHMIWLSPPWFVYVGTMIAFVPNLGFWTAYGLAIVLYLV
                     GVGLTRLFLS"
     gene            2712..3131
                     /locus_tag="PKM72_09530"
     CDS             2712..3131
                     /locus_tag="PKM72_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61069.1"
                     /translation="MAGFIKPIIHAFLVCDTIIIDSLTGKKSLIGAFTHLWAKTFPCQ
                     HPQVGVYFSLTDAEGRYAFEIQLVYLDQDQIVGKGSLPPIDISNRLMTQDFGVNIPYL
                     MFPGPGRYEFRLYADGHFITQKDFQVIQQESSPSPQS"
     gene            3492..4427
                     /locus_tag="PKM72_09535"
     CDS             3492..4427
                     /locus_tag="PKM72_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNP61070.1"
                     /translation="MAAFHHHQGKLRLRCLFILILFVLGTGTVNGQVVGDGYNNSLIY
                     KIQSDTNTVYILGSIHVLAEEYYPLTRSFSYAYYNSQKVVFEIDPEILFSPDSFSQHQ
                     KQYTLPKGKTLETVLSPKTYTLVKQKIKALGGKIQDFQQYKPWVLYLTLSGRFDSSID
                     FRPELGIENHFYRMAKDAGKPTGGLETIQDQLNVFDTLPFNIQDALLQESLAAAGSRE
                     REQAFLHMVKDWHQGNLDGLEQLVESFKTYPLFYKKLLVQRNQNWIPQIESFLQEEKN
                     VLIIVGAAHLAGKDGLLALLKEKGHQIERVSYAMP"
     gene            complement(4448..5257)
                     /locus_tag="PKM72_09540"
     CDS             complement(4448..5257)
                     /locus_tag="PKM72_09540"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP61071.1"
                     /translation="MNHFRNQVILITGASSGIGKALACALAPQAPKLVLVARDRSRLQ
                     EVALACENLGASALVIPTDVTDPHACSAMIQQVVTHYSRIDVLVNNAGISMWSTVEDA
                     HDVELYQRVMAVNFFGSVYCTKSALPFLKATQGRIVAISSVASFTGLPAHSAYCASKH
                     AMNGFFESLRIELAGTGVTVTIVAPDFVQSEIHERSIGPHGQPFGRILDGHSRFLPAE
                     KCADMIIKGMTLRKRLVFTSWRGQGGRWMKLMSPQMIDWIARKGMADADRP"
     gene            5819..6415
                     /locus_tag="PKM72_09545"
     CDS             5819..6415
                     /locus_tag="PKM72_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNP61072.1"
                     /translation="MSIPSTRFQPYCLELPGWITDYLGQQPETYPTPDARMKLVVKLA
                     QLNIQYGTGGPFGAGVFRMDTQELVAPGVNLVLSSKSSIAHAEIVAMMMAQQIVRSHN
                     LGAIGLPSHELVTSCEPCSMCLGAISWSGVRHIVCGARGSDAEAVGFDEGPKPANWMM
                     ELEQRGIAVTMDIGRTHASAVLQQYVQNGGVVYNGRRG"
     gene            6462..7292
                     /locus_tag="PKM72_09550"
     CDS             6462..7292
                     /locus_tag="PKM72_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNP61073.1"
                     /translation="MTPKQENSKGPHSPITLAIDIGGSGIKGLLLDQSGQPLSERVRM
                     VTPKPATPQAIVPLIVEIAQTLGQFDRVSVGFPGVIHQGWVKTAPNLDVSWPGTYLVG
                     ELEQQLKRPVRAANDADVQGYGAIKGQGVEMVITLGTGFGTALFINGHLVPNMEIAHH
                     PFRKQETYEVQLGAPALNNVGKKRWNLRLAKAIEILDRVINFDHLYIGGGNAKKVTIP
                     LPSHATIVPNTAGLLGGIALWADPNVVLPPTTKKPGKGTTRKQAPRKKSSNTRQKKRK
                     "
     gene            7362..7775
                     /gene="crcB"
                     /locus_tag="PKM72_09555"
     CDS             7362..7775
                     /gene="crcB"
                     /locus_tag="PKM72_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNP61074.1"
                     /translation="MTNRHTPTMIPYGLPQLLWVGLGGFLGSIGRFVVAGFFNRLIPT
                     LAFPIGTLAVNILGCFLIGLLHGVAESRNILGTDTRIFLFIGVLGGFTTFSTFGFESL
                     ALLKDGAMLKASANIIIHVFVGLAAVWLGDTLGRL"
     gene            7817..8635
                     /locus_tag="PKM72_09560"
     CDS             7817..8635
                     /locus_tag="PKM72_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61075.1"
                     /translation="MKFALLSFLFFGIGLLGCGTGNPKIHLTPEEGIQLKNASKVFLV
                     HYQSPDFFIWTGSSNLPRAIAGGLLSGALGGGFVGSGVMVKESWDNFRIQMNLQFFPH
                     DITEKMKQKFLEVLRKNYHFSNVTPISFQDEVSLMNLRENLGNDKSKNNKVIDIKTYF
                     WHLSPTYEKNKNNFQFQYRGEVRVINLQPLSVILQHQCQYPAHSRGDLILIPEGPSML
                     SGPVWEDSMTMPELITNNGEKIQTQIDKAVDHCLKEFTDKFFHEINDLSSSSPN"
     gene            8783..>9275
                     /locus_tag="PKM72_09565"
     CDS             8783..>9275
                     /locus_tag="PKM72_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNP61076.1"
                     /translation="MPKKKSVSNSTPPHTDLIIEGARQNNLKNISLRIPHNAVTVITG
                     VSGSGKSSLAFDTLFAEGQWRYVESLSSYTRMFLDRVKRPDVDRLTNIRPSIALEQKN
                     PIRTSRSTVGTTSEISDYLRLLFSKIGRLTCPDCKVEAIAHHPTTVAQDLLNRFPKER
                     ALIC"
BASE COUNT         2423 a         2439 c         2098 g         2315 t
ORIGIN      
        1 tcccctactc cacggtttac ctctcttcac cccaatttgt cactttttct cacacctatt
       61 taacacttgg cttttggcgt taagaatcct gaggggaggg actcctttcc cacagaaccg
      121 gaggaagatc atgaagagtc ttttggcgaa aagacatctc atggatggaa ggggcggaca
      181 aatttgatcc cgctcaacag aatgatggaa tcaaacagca aaccgtaatg ccgctacata
      241 gaaaactcag ttaacctaca ttagactatt cgctaacata cgaagaaccc tgagatgcct
      301 aaagggaata ccccaacctc taaggctctc aattgtgtat tagcagaaca agccggggta
      361 cgtcactctg gcacggattg aggcgggaac aattgacaat catctcgcca tgcttcagaa
      421 ggcgaccgcg gcattagggg ccgacacgaa aggctcaaaa ctggatcctg ctgaaacctt
      481 gcgctatcta taagagtatt cagttcattg gatttttaca aattgctatg aagaaacgat
      541 tgatgccttc catgaccctt gcaggcctgt gataccccag aaagaacgag cctcaggatt
      601 agcctttctc cctattttcg ccacaatcgg gttttacctc ttacccgcac actggcaact
      661 ccacactgcc gtccagtttg ctccacaact gatggcctat ggcgccttaa gcacctgggc
      721 atttaacaat ggggatcagc tccacaaact tgggctagga tttaacaaca tacaggcagg
      781 tattctccga gggactgtga ttggcatcac actgggaata ctgaataccg gggttatctt
      841 gaccgtcatg cctgcttggg gggtggacat caattttctt tcccagaccc ctcatgccca
      901 ggttccgttc tggatcatgt tgccgtggtt catcatcctg attgccatgg gggtggaatt
      961 gaatttccgc ggatttctcc tgggccgatt actgatatgg tttcaagatg tgcgtcaact
     1021 cgatcacccc tcgaccacca atatttgcct acagactttc ctccccttat ccatatcggc
     1081 tctgacgttt tcatttgatc cctttatggt gtcgacattc cgtgatttac attggattgc
     1141 catatgggat ggcctggtgt ggggatggct atggatgcga tggcacaatc tctatatggt
     1201 cattgcggct catgccgtgg aagtgattct tctctatctc atcatacggg gaatattgac
     1261 atgacaccct cgttttcgcc tcatctggtg aatcatccct ttggagaccc cggcctctat
     1321 gttgatatac gttggagccg gcgtgccctg ctctttgatc taggggagaa tgtgtcgttg
     1381 agccccaccc agcttctacg ggcacaggat attttcatct ctcacacgca catggatcac
     1441 tttatcgggt ttgaccggct tattcgtgtg gccctgggtc gtggcaaaca ccttcgattg
     1501 catgggccac cgggccttat tgaaaacatt caaggcaaac ttcgtggtta tacctggaat
     1561 ctggttgatg gatatccact caccatcgaa gccagagaat atcaccccac ccatatccaa
     1621 tccgggcatt ttcatgcgaa ggacgccttt gtctgtaaac aggagaactt aatcccaggc
     1681 gatcattccc acatttttcc ggttcttcaa gaaccggctc ttcttgtgaa agccgtcgcg
     1741 ctcaaccatc ggatcccgtc gctggctttt tccctggaag aaccgtttca catcaatatt
     1801 aaaaaagccc gactccatga ggctggtctt ccggtgggct cctggctcaa ggacgccaaa
     1861 caattaatag tagatggcgc accggattcg cacacctttt ccgccacgtt gtattttgaa
     1921 caccggaaag aagagcgcgt atttgatgtg ggcatcatga aacgggaatt cgtaacgatc
     1981 accaagggac aaaagatcgc ctatgtcgtg gattgccgtt atgatgccga caatgagcaa
     2041 cggattattc agttatccca aggggcaacg accttatttt gcgaggcacc atttttagaa
     2101 gccgaccggg acaaggctgc caatcgctac caccttacgg cccgccaggc aggaatcctc
     2161 ggacgaaaag ccggggtaca acaattggtc gtctttcatt tttctcccag gtataccggg
     2221 cagggagaca aacttcacca ggaggccatg gaggccttcc atgaacaata attacacatg
     2281 acgcgaggag ggacgatgcc ggaatgggtt agatatgggt tgtattttgt cctgggcgga
     2341 acgttagtga gtgtctccac atatctggga agtcatggga gaggattcct ggctgccttg
     2401 gccagcactc tgccggtcat cagcggagtg acttttatcc tgatcttcct caatgccgga
     2461 ccggctccga ccgtgtcgtt cgccaaacac atgatttggc tttctccccc atggtttgtc
     2521 tatgtgggaa cgatgattgc gtttgtcccc aatttaggct tttggacggc atatggtttg
     2581 gcgatcgtac tatatttggt gggagtgggg ctgacccgac tgtttcttag ctaacagaaa
     2641 aaccccggca ttggataatc aatagaagag tcttcgaata atcatgtgaa aagatagaga
     2701 ggacaaagaa tatggcgggg tttattaaac cgatcattca cgcattttta gtatgtgaca
     2761 ccattatcat cgatagtctg acagggaaaa agagtctcat tggtgcattt acgcatctct
     2821 gggccaaaac gttcccctgc cagcacccac aagtcggcgt gtatttttcg ctcaccgatg
     2881 cagaaggcag gtacgcattt gaaatccaac tggtgtacct tgatcaggac caaattgtcg
     2941 gcaaaggttc cctcccgccc attgacatat ccaaccgctt aatgacccag gactttgggg
     3001 tgaacattcc ctacctcatg tttcctgggc cgggacggta tgaatttcgt ctgtatgccg
     3061 atggtcattt catcacccaa aaggacttcc aggttatcca acaagaatcc tctccctccc
     3121 cacagtcctg actccgtatc agctttctcc tctcaccaag acatctagcc ccccttcgta
     3181 tgaagtgaga gacgggaaat ccggcagagc tcattccact attcggataa cctgatcaat
     3241 atcggtaaat agacctggca ggaacatgcc cattcttgtt gaattcgaca agaaccagtc
     3301 gcacattcca tcaatctcga tatcgcaact taatctctgc atttcacctt ttccctccaa
     3361 ccaccgatga attacccgga aagaaacacc cgacgaatat aagaacccaa agaaaatgcc
     3421 ggacaccggg gcacataact ctctcacacc caaatcagga cctgatcaca ccttcaacag
     3481 ggggatttga aatggctgca ttccatcacc atcaagggaa actacgccta cgctgtctgt
     3541 tcattctgat cctctttgtt ttgggcactg ggacggtcaa tggccaggtg gtcggggatg
     3601 ggtacaacaa cagcctgatt tacaaaatcc aatctgacac gaataccgtc tatattttgg
     3661 gttcgatcca tgtattggcc gaagaatatt atcctctcac cagatccttc tcctatgctt
     3721 attacaattc acaaaaggtc gtctttgaaa ttgatccgga aatacttttt tctcccgatt
     3781 cattttctca acaccaaaaa cagtacaccc tcccaaaagg gaaaacactg gaaacggtgc
     3841 tctcaccaaa aacctatacg ctggtcaaac agaaaattaa agccttggga gggaaaatcc
     3901 aggatttcca acaatacaaa ccctgggtac tctatctcac gctaagcgga cgatttgatt
     3961 cttccataga tttccggcct gaattaggaa ttgaaaatca tttctatcgc atggccaagg
     4021 atgccggaaa acccacgggc gggctggaaa ccattcaaga ccaattgaat gtgttcgata
     4081 ctcttccgtt caacatccag gatgctcttc ttcaggaatc actcgccgcg gcggggtcaa
     4141 gggaacgaga gcaagcgttt ttacatatgg tgaaagactg gcaccaggga aatctcgacg
     4201 ggttggaaca attggtggag tcattcaaaa cctacccgct gttctacaaa aaacttctcg
     4261 tccaacgaaa ccagaactgg attccccaaa tagaatcgtt cttacaagag gagaaaaacg
     4321 tcttgatcat tgtgggtgcg gctcaccttg cgggaaaaga tggattactc gcactgctga
     4381 aagaaaaagg ccaccagatt gaacgagtgt cttatgccat gccctaaagc ggcaaaggaa
     4441 tctcccgtta cggccgatca gcatcggcca tgccttttcg ggctatccaa tcaatcatct
     4501 gcgggctcat taatttcatc catcggcccc cttgtcctct ccaggatgta aacaccagtc
     4561 gtttccgcag ggtcatgccc ttgatgatca tgtccgcaca tttctcggcg ggtaaaaatc
     4621 tggaatggcc atcaagtatt cgcccgaagg gttgaccgtg aggcccgata cttcgttcat
     4681 gaatttcgga ttgcacgaaa tcgggggcca caatggttac ggtgactccg gttcctgcca
     4741 actcaatacg aagcgactca aaaaatccgt tcatcgcatg cttactggca caataggctg
     4801 aatgagcagg caatcccgta aaacttgcaa cgctggagat tgcaacaatc ctgccttgcg
     4861 tggcctttaa aaacggcaac gcagatttgg tacagtacac gctgccgaaa aaattcaccg
     4921 ccatcacgcg ttgatacagt tccacgtcgt gagcatcctc caccgtcgac cacatggata
     4981 tcccggcatt attcaccaac acatcaatcc gggaataatg agtcaccacc tgttgaatca
     5041 tcgcggaaca ggcatgggga tccgtcacgt ccgtcggaat gaccagtgca gacgctccca
     5101 aattttcaca ggcgagtgcg acctcctgca agcgtgaccg atctctcgca acaaggacca
     5161 gcttgggagc ctgtggcgcc aacgcacaag cgagcgcttt tccaatcccc gaagacgccc
     5221 cggtgatgag aatcacctgg tttctaaaat gattcatcgt ctctttccca tagattgata
     5281 acgggctaga ccctattgac agatttttct gacgagcgta gcattacttc tgcgctcata
     5341 aaagccgatt atgaatggaa ggagagaatg agcgtgcatc tcctttcaaa ccgtaaagag
     5401 gaaagggggt ctcagatgga cgaccttact ccaccggatt attttggccc atcggcggca
     5461 aaacccgcct cagtctcctg acgggccttc tcgtttttgc cggatcacat ccctgctcca
     5521 acacaagcat gccaaaaggc atttgccagt gggcacccta ccgagcttgg aagtgagtca
     5581 caagagggat cttcaacctt cataactgga agattcggcc aaaccataaa cgagaaacgc
     5641 gtcattagcc tactgatggg ccatgctaga catgaggttc actgtttgtg gtgtatacca
     5701 cataatccga ggggctccct ttcacgggac aagcccctcg ggttgttttt cagaccggca
     5761 ccacaatccc ataaccgttc ttcacacctt ctgccactca aacccctgac tccctgatat
     5821 gagcatccct tctacacggt ttcaaccata ttgccttgag ttaccaggct ggatcaccga
     5881 ttatcttgga caacaaccgg agacataccc gactccggat gctcgtatga aattggtcgt
     5941 taagctggca caactcaaca ttcaatatgg aacaggggga ccatttgggg caggagtttt
     6001 ccgcatggat actcaagaat tggtcgcgcc aggggtgaat cttgttcttt cgtcgaaaag
     6061 ttcaattgcc catgctgaaa tcgtcgcgat gatgatggca cagcaaatcg ttcgttccca
     6121 taatttaggg gcaatcgggc ttcccagcca tgagctggtt accagttgcg agccctgcag
     6181 tatgtgcttg ggcgcgattt cctggtccgg tgttcgacac atcgtctgtg gcgccagagg
     6241 aagcgacgct gaagcggttg gatttgatga aggccccaaa cctgcgaatt ggatgatgga
     6301 acttgaacag aggggaattg cggtcaccat ggatatcggg cgaactcatg cctccgcagt
     6361 cttacaacaa tatgttcaga atggcggagt agtgtataac ggtcgacgag gctagatggg
     6421 gaccatttca acttcttttt aaacttcgag gagatactcc catgactccc aaacaagaaa
     6481 attcaaaggg cccccactca cccatcaccc tcgccattga tattggcgga tccggaatca
     6541 aaggcctcct gctcgatcag tccggtcaac ctctttctga acgggtacga atggtcacac
     6601 cgaaacccgc aacaccacag gccattgtcc cgcttattgt ggaaattgcc caaacactcg
     6661 ggcaatttga tcgcgtatca gtcggctttc ccggcgtaat tcatcagggg tgggtgaaaa
     6721 cagcgccaaa cctggatgta tcctggcccg gaacctatct cgttggcgaa ctggaacaac
     6781 agctcaaaag gccggttcgg gcagcaaatg atgcggatgt gcagggttat ggcgcgatca
     6841 aaggccaagg ggtcgaaatg gtcattaccc tgggaaccgg atttggcacc gcgctattca
     6901 ttaatgggca cctcgtgcct aatatggaaa ttgctcacca cccctttcga aaacaagaga
     6961 cctacgaagt acaattgggt gcccctgcac tcaacaatgt ggggaaaaag cgatggaatc
     7021 tccgtctggc caaagccatt gagatattag atcgagtgat taatttcgac catctctata
     7081 taggcggggg caatgcaaaa aaggtcacca tcccactgcc atctcatgcc acaatcgtcc
     7141 caaatacagc aggtcttctt gggggaatcg cgctctgggc cgatcccaat gtggtccttc
     7201 caccgacaac caagaagcct ggcaaaggga ccacacgaaa acaggctcct cgaaaaaagt
     7261 catctaacac tcgacagaaa aaaaggaaat gactcccata cacgactcac cacccagttc
     7321 gccccacgcc aaatcgcgcc ggaccttcat cctgaaggaa attgactaac cggcacacgc
     7381 caaccatgat tccttacgga ttaccgcaac ttctctgggt aggtctgggt gggttcttag
     7441 gttcgatagg ccgatttgta gtcgccggct tctttaaccg cctgatcccg actttggcct
     7501 tccccattgg cacgttggcc gtaaacatac tcggctgttt cctgatcggg ttactccatg
     7561 gagtggccga gtccaggaat atcctgggaa cggacaccag aatttttctg tttattggcg
     7621 ttctgggggg attcacgacc ttctcgacct tcgggttcga atccctggca ctcctgaaag
     7681 acggggccat gctcaaagcc tccgccaata tcatcatcca tgtcttcgtg ggtctagccg
     7741 ctgtctggct aggtgataca ttaggccgtc tatgatcatc atcagacccc agtgaggcat
     7801 ttccctcaag attacgatga agtttgctct attatctttt ctcttttttg gaattggatt
     7861 gttgggatgc ggaacaggca accctaaaat ccaccttact ccagaggaag gaattcaact
     7921 caaaaatgct tctaaagttt ttttggtcca ctatcaatct cctgattttt tcatatggac
     7981 aggcagttcg aaccttccaa gagccatcgc agggggactg ctaagcggag ccttgggcgg
     8041 aggatttgtt ggctcggggg taatggttaa ggaaagttgg gataatttta gaatacaaat
     8101 gaaccttcaa ttttttcctc atgacattac agaaaaaatg aaacaaaagt ttttggaagt
     8161 tttaagaaaa aattaccatt tttccaacgt gactccaatc tcctttcagg atgaggttag
     8221 cttaatgaat ctgagagaaa atcttgggaa tgacaaatct aagaataaca aagttattga
     8281 cattaaaaca tatttttggc atctgtcgcc cacatacgag aaaaacaaaa acaatttcca
     8341 gttccaatat aggggagaag tccgggttat taaccttcaa cctctttcgg taatactcca
     8401 acatcaatgc caatatccag cacacagtcg gggtgatttg attttaatac cagagggtcc
     8461 ttccatgctg tcaggccccg tttgggaaga ctccatgaca atgccagagt taataaccaa
     8521 taacggagaa aagattcaga cacaaattga caaagccgtg gatcattgcc tcaaagagtt
     8581 taccgacaaa tttttccatg aaataaacga tctgagcagc tcatcaccta attaaggaaa
     8641 ctcgtttccc ctccagcttt tccattttct tcataaagcc tagtgtgttc acctttcctt
     8701 aaagctcttg gctaagcctg ggcaatcaag gtacaaacca tgtattgtct tttaactatt
     8761 ttgattgtcc accgtcatac ctatgcccaa aaaaaagtcc gtttccaact ccacgcctcc
     8821 tcacaccgac ctgatcattg aaggcgcgcg gcaaaataac ctcaaaaaca tttctctccg
     8881 tattccacac aacgccgtca ccgtcatcac tggtgtgtcc ggatccggga aatcatcttt
     8941 agccttcgac acactgtttg cggagggcca atggcgttat gtggaatcgc tctcgtccta
     9001 tacgcgcatg ttcctggacc gcgtcaaacg gcctgatgtg gaccgactca ccaatatccg
     9061 tccctcaatt gccttggaac aaaaaaatcc gattcgaacc tcacgctcca ccgtcggcac
     9121 gaccagtgaa atttccgatt atctccggtt gctgttttcc aaaatcggac gcctgacctg
     9181 tcccgactgc aaggtggaag ccatcgccca ccaccccact acggtcgctc aagacttact
     9241 gaatcgtttt cccaaggaac gcgccctcat ctgtt
//
LOCUS       DAORAQ010000103         9241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1188_length_9241_cov_57.337035, whole genome shotgun sequence.
ACCESSION   DAORAQ010000103 DAORAQ010000000
VERSION     DAORAQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1188_length_9241_cov_57.337035"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKM72_09570"
     CDS             complement(<1..283)
                     /locus_tag="PKM72_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNP61077.1"
                     /translation="MKTITRIGIGGPVGSGKTAIVEAITPKLMDMGGRILIITNDIVT
                     TEDAKHVQRTLKGILLEDRILGVETGSCPHTAVREDPSMNIAAVEDMEAR"
     gene            complement(302..991)
                     /locus_tag="PKM72_09575"
     CDS             complement(302..991)
                     /locus_tag="PKM72_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HNP61078.1"
                     /translation="MLTISELMRVLQFGDSVLPVGSFTFSNGLESAVEHGVVHDLASL
                     RQFVRIAVEQAATSDGIAVLAAFRAARAGDNARIDEADHAVFNRKLNEEIRTMTVRMG
                     RKLVEMADRVVGPTVASAWLCRITAHETPGCFPIGQALVFASLGLSEQDAFAVHQYGL
                     ASMMLAASLRLMKLHYLDAQAVLFEINSEAEVAYQRIAACSLEDMATFVPVIDILASS
                     HVQAKVRMFMN"
     gene            complement(1037..1642)
                     /locus_tag="PKM72_09580"
     CDS             complement(1037..1642)
                     /locus_tag="PKM72_09580"
                     /inference="COORDINATES: protein motif:HMM:NF017045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61079.1"
                     /translation="MIMVESVLGNVKDAGWKAKLDGAHVDVMNLDQWQAQKTRFRLKS
                     EGGIELAVSLERNTHLHDGDILIWDDTTREAIIARIQLQDVMVIRLDGLLSKTQDFLA
                     HICLELGHALGNQHWPAVVKGTTVYVPLTVDRQVMASVMKTHAFEEITYEFSPGASVI
                     PYLAPHESRRLFGGANSTPHSHVHEHSPTLNHHDDHSHPHT"
     gene            complement(1764..3482)
                     /locus_tag="PKM72_09585"
     CDS             complement(1764..3482)
                     /locus_tag="PKM72_09585"
                     /inference="COORDINATES: protein motif:HMM:NF009834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNP61080.1"
                     /translation="MSKISRKQYADLYGPTTGDKIRLGNTDLYVEIEKDLRVYGDELV
                     TGGGKTLRDGMGSDNQITQAAGCLDLVITNVTILDPILGVIKADVGVRNGRIVGVGKA
                     GNPSTMDGVTPGLTTGTSTDAITGEHLILTAAGMDTHIHFICPQQIWHGLSNGITTLW
                     GGGIGPTDGTNGVTTTNGPWNMEMMLRSVEGLPVNVGFYGKGNCTGKAPLVEQLEAGA
                     TGFKIHEDYGTTPAAIRSALSVAEEYDVSVAVHTDTLNEGGFVEDSIAAFDGRTIHTY
                     HSEGAGGGHAPDLLKVVGQPNVLPSSTNPTLPCGVNSVAELFDMIMVCHNLNPKIPSD
                     VAFAESRVRGETIVAESVLHDMGAISMIGSDSQAMGRIGENFLRAFQTADAMKQARGK
                     LPEDAPGNDNFRVLRYLSKVTSAPCIAAGIFHQLGSIEPGKLADLVLWEPAFFGAKPK
                     LVLKGGFVVWANMGDPNASLPTPQPMMYRPMFGALGSTLQSTCMTFVSQVAHDRNVKE
                     KYGLGRMVEPVRQTRVLTKKHMVRNDYLPKIEVNPQTFAVMVDGVHATVTPPKNIALN
                     QLYFFS"
     gene            complement(3512..3997)
                     /locus_tag="PKM72_09590"
     CDS             complement(3512..3997)
                     /locus_tag="PKM72_09590"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNP61081.1"
                     /translation="MAQSKKDTPNSGVGKWLRHKHAGPQSEQTDTASVGGLVLAKGDI
                     EINAGRPRTTLRVRNTGDRPIQVGSHFHFFESNRYLQFDRGQAFGLRLDIPATTAIRF
                     EPGDEKEVTLVPMGGKQFCVGFNNLVDGWTGDGPTPDYRPNYDEAMRRMKERGFKSTK
                     S"
     gene            complement(4033..4335)
                     /locus_tag="PKM72_09595"
     CDS             complement(4033..4335)
                     /locus_tag="PKM72_09595"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNP61082.1"
                     /translation="MHLTPRETEKLLIYQLGDIAQRRKTKGLKLNHPESMALICSTAL
                     EGAREGKTVEEVMTEAAGVLTRDDVMEGVAEMIPFVQLEAVFTDGTRLITVHDPIK"
     gene            complement(4432..5352)
                     /locus_tag="PKM72_09600"
     CDS             complement(4432..5352)
                     /locus_tag="PKM72_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNP61083.1"
                     /translation="MATESILNDRQTLWDPGNFFRIVLRGIGQVMFQGHAGTGILFLL
                     GIAFASPLMAVGALIGALIGPVTATLARFDGEEIEEGLHGFNPTLVGIASLFYLKPEP
                     LTWVLLIVGCVASTFLTFVMRQYVKFPTYTAPFVLVTWVVMIVAHAMAGTAIDVMPAS
                     AANTPSGFVTAVLAGAAEVMFGATVLTGMLFLAGIALCTWRHAVLGLLGSIVGTLVAL
                     YHNDPSPAVHLGIYGYNAVLAPIAVYLWGKSLLIAILAAMISVPLTEFFPSWLGIPAL
                     TAPFVVASWIIIAVGQIDALFLREPVEKLG"
     gene            complement(5365..6567)
                     /locus_tag="PKM72_09605"
     CDS             complement(5365..6567)
                     /locus_tag="PKM72_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61084.1"
                     /translation="MLKPVKPGIISLFFIILLLVVLCTPDVYAAGKIEIDDTKWISLG
                     LGLRTSFTAQEDKAPNRNWGNDFNLDNLRLYVNGQIHKYIKVEFNTECDNCTSSDPTR
                     GGNMIILDAIGKFEFNQYFNIWAGRQLVPQHRAELDGPFFQNVYNFTQTPFYTADFSG
                     SIGQGGRFGRDDGVNIWGALTPDRRLTYVLGVFQGHRGGSNQSDSLLYAGRISYNFLN
                     IEQNPGYYTSSTYYGKGGDILTAAYVLQYQNNGAGTQASPSTFYGMNGDLLFEKVLGN
                     KGVLTVEGELKYFNPNLSDQALADPNCFCIFSGLSWMTTGLYLFPQKVGIGQFQPYVR
                     YVDTNPNESSSRYLVEGGLNYVIDGHNARISLFYQYGDFLTKGRTYTPGVKGDAVSAV
                     GLGLQLQL"
     gene            7275..8135
                     /locus_tag="PKM72_09610"
     CDS             7275..8135
                     /locus_tag="PKM72_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61085.1"
                     /translation="MQAWTGNMWRMSMAGLLGLAIVLGCAGPGIQTPIHKDETTRVYL
                     EWVPQDSFRATHPEVLSPTVIRRALRGLRVQKQEPAVAELLVGLPEPQRIFSDNDVEL
                     LTPHILLALSQATSEEHVVFQRIYPWKYGSRLTSGTLSLHEDLIFLTITHYTQNPGGI
                     NVVYVDDRQAPDPTGLGGWTVLFLPKEVLRADSSPQIWRHPHEVTLAINYPSLEMWRE
                     PSEQPSPSQVPPTEESSDPVKPEIIPGESASEDNAIRALKEQVHRQQMEMDQLRKEFR
                     ELQQSPKMTE"
BASE COUNT         2245 a         2594 c         2330 g         2072 t
ORIGIN      
        1 accgtgcttc catatcctcg acggcagcaa tgttcatgct cggatcttcg cgaacggccg
       61 tatgcggaca tgatccggtt tcgacgccca ggatacgatc ctctaacagg atacccttca
      121 gcgtgcgttg gacgtgcttg gcatcttcgg tggtcacgat gtcattggtg ataatcagaa
      181 ttcgccctcc catgtccatc agtttcggcg tgatcgcctc gacaatggcc gttttgcctg
      241 atccgacagg gccaccgatt cctatccgtg tgatggtctt catgcgtgtt ctccttactc
      301 atcaattcat aaacatgcgt acctttgcct gcacatgcga agatgccaga atgtcgatca
      361 ccggcacgaa ggttgccatg tcctcaagtg agcaagcggc aatacgttgg taggcgacct
      421 cggcctcgga gttaatctca aagaggacag cctgggcatc caggtaatgc aacttcatga
      481 ggcgcagcga tgcagcaagc atcatactgg ccagaccata ctggtgcacc gcgaaggcgt
      541 cttgctccga aagcccaagc gaggcaaaaa ccagcgcctg gccaatcgga aaacaccctg
      601 gcgtctcatg agccgtaatc cggcacagcc acgcactggc aaccgtaggc cccaccacac
      661 gatcagccat ctccaccagc ttgcggccca tacgcacggt catcgttcgt atctcctcgt
      721 tcagtttgcg attaaagacc gcatggtccg cctcgtcgat ccgggcattg tcgcccgcac
      781 gggctgcgcg gaaggcggca agcaccgcga tgccatccga ggtcgctgcc tgttcgacgg
      841 cgattcgcac aaactgtcgt agcgaggcga ggtcatgcac caccccatgc tccacggccg
      901 actccaatcc attggagaac gtaaatgacc cgaccggcaa caccgaatcg ccgaactgca
      961 gaacacgcat cagctcggag atggtcagca tgatcgtctt cctatcgaat ggtggcgtgt
     1021 ggtcgtcgtt cgtcgtctac gtatgtggat gggaatgatc gtcatggtga ttcagggtag
     1081 gcgaatgttc atgtacatga gaatggggtg ttgaattggc tccaccaaac agccgccggg
     1141 actcgtgtgg cgccagatag ggaatgaccg aggcacccgg cgaaaattcg taggtgatct
     1201 cctcgaacgc gtgggtcttc atcacggaag ccatcacttg gcggtccacg gtcaggggga
     1261 cgtagaccgt tgtgcccttg accaccgctg gccaatgttg attgcccagc gcatgaccga
     1321 gttcgaggca aatgtgagcg aggaaatcct gcgtcttcga gagcaaccca tccaaccgga
     1381 tcaccatcac atcctgaagt tgaatgcggg caatgatggc ctcacgggtg gtgtcatccc
     1441 agatcagaat gtctccatcg tgcaaatgag tattccgctc cagagacaca gccaattcaa
     1501 ttcccccctc cgacttcagg cggaaccggg ttttctgcgc ctgccattgg tcgaggttca
     1561 tcacgtcaac atgcgcacca tcgagtttgg ctttccagcc cgcatccttg acgttgccta
     1621 ataccgattc aaccataatc attgtttttc cccaatcgct aattcttgct cgcgcgccga
     1681 tgaaccttct tggaaattcc tggagcccgg ataccagtca gacgaaaccg actggtgccg
     1741 gacgcaggag tcattgatac gaatcaactg aagaagtaca actggttgag tgcaatattt
     1801 ttcggcggcg tgacggtggc atgtaccccg tcaaccatga cggcaaacgt ctgcgggttg
     1861 acctcgatct ttggcaaata atcattgcgg accatgtgtt tcttggtcag gaccctggtc
     1921 tgtctgaccg gctcgaccat acggcccaac ccgtacttct ccttgacgtt cctgtcgtga
     1981 gcaacctgag acacaaacgt catgcaggta ctctgcagtg tcgagcccag ggccccgaac
     2041 atggggcggt acatcatcgg ctgcggggtc ggcagcgagg cattcggatc ccccatgttg
     2101 gcccacacca caaagccacc cttgagcacc aacttgggtt ttgccccgaa gaaggcgggt
     2161 tcccagagaa ccagatcggc cagtttgcca ggctcgatcg atcccaactg atggaagatc
     2221 cccgccgcga tgcagggagc cgacgtcacc tttgacaggt aacgcaacac ccggaagttg
     2281 tcgttgcctg gagcgtcctc gggaagcttg ccgcgtgcct gcttcatggc atcagccgtt
     2341 tggaaggccc gcaggaaatt ctctccaatt cggcccatgg cctgcgagtc gctgccgatc
     2401 atcgagattg cccccatgtc atgcaacacg ctctcggcca cgatcgtctc gccacgcacc
     2461 cggctctcgg caaaggccac gtccgagggg atcttcgggt tcaagttgtg gcaaaccatg
     2521 atcatgtcga agagttcagc caccgagttt acaccgcagg gcaacgttgg attggtcgag
     2581 ctcggcagca cgttcggctg gccgacgacc ttcaagaggt caggcgcatg accgcctccg
     2641 gccccctcag agtgatatgt gtggatcgtt cgcccgtcga aggctgcgat ggagtcctcg
     2701 acaaatcccc cctcattgag cgtgtccgta tgcacggcca cgctgacgtc gtattcctcg
     2761 gccaccgaca gggcagaccg gatggcggca ggggtcgtgc cgtagtcttc atgaattttg
     2821 aaaccagtcg caccggcttc taactgctca accagcggtg ccttgccggt acaatttccc
     2881 ttgccataaa agcccacgtt caccggcaat ccctcaactg agcggagcat catctccata
     2941 ttccacggac cattcgtcgt cgtcacgcca ttagtcccgt cggtcggacc gatgccgccg
     3001 ccccagaggg tggtgatgcc attcgacagc ccgtgccaga tctgttgcgg gcaaataaaa
     3061 tgaatgtgcg tgtccatacc tgctgcggtc aggatgagat gctcgccggt aatcgcgtcc
     3121 gtcgaggtcc ccgtggtcag tcccggagtg accccgtcca tcgtcgaagg attgccggcc
     3181 ttcccgacgc cgacgatccg cccattgcgg acaccgacgt ctgccttaat cacgcccaaa
     3241 atcggatcca gaatggtcac gttggtgatg acgaggtcga gacagccggc agcctgggtt
     3301 atttgattgt ccgaccccat tccatcacgg agagtcttac cgcccccggt gaccaactca
     3361 tcgccataca cacgcagatc tttttcgatc tcgacgtaaa gatccgtatt gcccaatcgg
     3421 attttgtcgc ccgtagttgg accatacagg tcggcatatt gtttgcgtga aatcttagac
     3481 atctcagaga ctcctttttt gtcggtgttg gtcaggattt ggtcgacttg aacccacgtt
     3541 ccttcatccg ccgcatcgcc tcgtcatagt tgggacgata gtcgggtgtt ggcccgtcgc
     3601 ccgtccaccc atcgaccagg ttgttgaagc cgacgcagaa ctgcttccca cccatgggga
     3661 cgagtgtcac ctccttctcg tcgccaggct cgaaccggat ggcggtcgtt gccggaatat
     3721 ccaaccgcag accaaatgcc tgcccccgat caaactgcag gtaacgattg gattcgaaaa
     3781 agtggaaatg ggaaccgact tggatgggcc ggtccccggt gttccggact ctcagagtcg
     3841 tcctcggccg gccggcattg atttcaatat cccctttggc cagaaccaga cctccaaccg
     3901 aagcagtgtc cgtctgttcg gattgagggc cggcatgctt gtgccgcaac cacttcccga
     3961 ctccagagtt gggggtgtcc ttttttgact gggccatgtt cgtcctcctc tttgtctcag
     4021 aatgaatagg gattacttga tcggatcatg gaccgtaatc agcctggtgc cgtcagtgaa
     4081 cacggcctca agttgaacga aagggatcat ctcggctact ccctccatga cgtcatcacg
     4141 tgtcagcacg cccgcggcct ccgtcatcac ctcctcgacc gtcttcccct cgcgggcccc
     4201 ttcgagagcc gtcgagcaga tgagcgccat cgactccgga tgattcagct tcaggccttt
     4261 ggtctttcgc cgttgggcaa tatcgccaag ttggtaaatc aatagttttt ccgtctcacg
     4321 aggtgttaga tgcataacat gctctcctct tcaatagtga acgtgtgatg taagagatcc
     4381 ggcgcccgca atccacgaaa cgatccaagc gaccccctgt gcctgttgac gttaacccaa
     4441 cttctccacc ggctctcgca gaaataacgc atcgatctga ccgacggcga taataatcca
     4501 tgacgcgacc acaaaggggg cagtcaacgc ggggatgccc aaccaggagg gaaagaactc
     4561 cgtcaaaggc acggagatca tggcagccag aatcgcaatc aggagagact tcccccagag
     4621 atataccgca atcggtgcca gaacagcatt gtatccatag atgcccaggt gaacggctgg
     4681 gcttggatca ttgtgataca gtgccacaag cgttccaacg atggatccca ataggccaag
     4741 cacagcatgc cgccaggtgc agagcgcaat ccccgcaaga aacagcattc cggtcagcac
     4801 cgtcgcaccg aacatgactt cggctgcacc cgctaaaacc gccgtgacaa aaccagacgg
     4861 cgtgttcgcc gcagaagccg gcatcacgtc gatcgcagtt ccggccatcg catgcgcaac
     4921 gatcatgacc acccacgtga cgaggacgaa gggtgcggtg taggtgggga atttcacgta
     4981 ttgacgcatc acgaaagtga gaaaggtcga ggccacgcac ccgacgatta gcagaaccca
     5041 agtcagaggc tcaggtttta ggtaaaagag tgaggcaatc cccaccagag tcgggttgaa
     5101 gccatgcagt ccttcctcga tctcctcgcc gtcgaatcgc gccagcgtcg cagtgaccgg
     5161 accaatgagg gccccaatca gcgcgcctac cgccatcaac ggagacgcga atgcgatccc
     5221 aagcagaaat aaaatccccg ttccagcatg gccctggaac atcacctgac caatcccgcg
     5281 aaggacgatc cggaagaaat tgcccggatc ccataacgtc tgccgatcgt tcaggatgga
     5341 ctccgttgcc atgtcagtcc cccgtcacag ttgaagttga aggcccagcc ccacagcact
     5401 gaccgcatcg cccttgacgc ctggggtata ggtcctgcct ttggttaaaa aatcgccata
     5461 ttgataaaac agcgaaatcc tcgcgttatg cccatcgatc acatagttca atcccccctc
     5521 aaccaggtat cgggaggagc tctcattagg gttggtgtcc acatagcgca cgtaaggctg
     5581 aaattgcccg ataccgactt tttgcggaaa aagatacaag cccgttgtca tccaggaaag
     5641 accggaaaag atgcagaaac aattggggtc agccaatgcc tgatcgctca ggttggggtt
     5701 gaaatacttg agttcaccct cgacggtcag cacacctttg ttgcccagca ccttttcgaa
     5761 taatagatcc ccattcattc cgtaaaaggt cgaaggagag gcttgagtgc cggctccatt
     5821 gttttggtat tgcaacacat aggcggcggt caatatatcg ccgcccttgc cgtaataggt
     5881 gctgctggtg taatagcccg gattctgttc gatgttgaga aagttgtaac tgatgcgtcc
     5941 agcatacagc aggctgtctg actgattcga cccgccgcgg tgcccttgaa atactcccag
     6001 gacatacgtc aatcgcctat ccggagttaa tgctccccaa atgttcacac cgtcgtcgcg
     6061 gccgaaccgc cccccctgcc caatactccc ggagaaatcc gcagtgtaaa agggggtttg
     6121 agtaaagtta taaacatttt gaaaaaacgg gccgtccagt tcggcacggt gctgtggaac
     6181 taactgacgt cctgcccaga tattgaaata ctgattaaac tcaaacttgc cgatggcatc
     6241 aaggataatc atatttccac cgcgggtagg atcggacgac gtacaattgt cacattcggt
     6301 gttaaactcg accttgatat atttatgaat ctgcccattc acgtagagac ggagattgtc
     6361 cagattaaag tcattgcccc aatttctatt cggcgcctta tcttcctgag cagtaaaact
     6421 cgtccgtaag cccaacccca aactgatcca tttcgtgtcg tctatctcta ttttccccgc
     6481 cgcatagaca tcgggtgtgc ataacaccac cagaaggaga ataatgaaaa agagactgat
     6541 tattcccggt ttgactggtt ttagcatgcg ctctcttcct gatctaaacg acaaaagtgc
     6601 gggggggggg tagccggatg aaacaaaaca tatatccgtg gattacaaaa aataattcat
     6661 gtaaaacaca gcaaccataa atacttggaa ccatgctgga attttgcttc ttatagcaga
     6721 taattctaat taatactata aaaatccctt gaagccgccc tagggaaact ttgcttaata
     6781 aaaaggctga acgtagagcg tgcatcaata agaaccctta tacaagatca ccgaaagatc
     6841 ctgaaaggaa aagctgccaa tgtccggggt gtttttatac tttaagatct aattttcaga
     6901 gtggtgaact agacgagtgg agcggtattg gaggactcgc acctgaatat gctctcaccc
     6961 acaggcttga tcacgccaag acgaagaatt gaaaaatgac caatgggact gtccaaaatt
     7021 caatgggagt atttgcaaac agctaagttg tgaaatacaa aaagtgacaa agatttcacc
     7081 aggaattaac atgtgagaaa ttgcgattct cggacctgat gcccttttca aagccaagcg
     7141 agttttatga tttggtgacg aaagatcaaa catttctttt tcatttaatg ggaaacgaac
     7201 gaatcggtgc catcctgaag catacatttc cgggtggttg ttgacgcaga cgaaaaagtc
     7261 gtcgggaaag tgacatgcaa gcctggacag gaaatatgtg gcggatgagc atggcaggat
     7321 tgttgggcct cgcgattgtg cttggttgcg ccgggcctgg gatacagact cccattcaca
     7381 aggacgagac cacgcgcgtg tatctggaat gggttccaca agattccttt cgggccaccc
     7441 atccagaggt tctctccccg acggtaatcc gtcgagcgtt acggggatta agggtacaaa
     7501 aacaagaacc agccgttgcc gaactgttgg tcgggttgcc ggagccgcag agaatttttt
     7561 cggataatga tgtggagctt ctgactcctc atattctttt ggcattgtcc caggccacat
     7621 ctgaagagca cgtggtcttt caaagaatct atccttggaa atacggttcc aggttgacga
     7681 gtgggactct ctccctccac gaggacctga tatttctgac catcacccat tacacacaga
     7741 acccgggagg aataaacgtt gtgtacgtgg atgaccggca ggctccggat ccgaccggcc
     7801 ttggcggttg gaccgtgctg ttcctcccta aggaagtgct gagggccgac tcctcccctc
     7861 agatctggcg tcacccccac gaagttactc tggcgatcaa ttacccgtct ctggaaatgt
     7921 ggcgagagcc gtccgaacag ccctcccctt ctcaggtccc gccaacggaa gaatcctcag
     7981 accccgtcaa acccgaaatc atcccgggag aatcggcatc ggaagataat gccattcgtg
     8041 cgttgaagga acaggttcac aggcaacaaa tggaaatgga ccaattaaga aaagaatttc
     8101 gagagcttca acagagtcct aaaatgacag aataaactca cggccggtcc atcttctcat
     8161 agagaaaaaa tgttcggact gagcaaagga gaaatctggc cctgccaaac tgatttcctg
     8221 aattccctcc aaatccgacg ggagttgttg ggctgagtct ggcacaaacc tcaccattcg
     8281 ggatggcaca tccactcgtc ccattccacc tgattgcagt tgacctttgg cggaatgtgt
     8341 gtggtgacat tcaagtgatc gccccctgcg gaattgacat ccgacatgga catgctcatc
     8401 agaaccatga ttgtaatttt tgaaggttca tttgcgttga aaggccttcc ggcctcgcac
     8461 agggcatctt tctatccata gcccggacag aagagccatt tccatcgctt ggacgggtcg
     8521 cgtaccaaac tgtttgtagt cggctttgta catgggacca ccagaagtgc tgccgcaaac
     8581 cgcaaccacc atgccgtcaa ccttaatggg catggcttgc ctttttaggg catctttgtt
     8641 aggggtttac gtacttatcg aaacactgcg gctcataatg cgcatattta caaactgcga
     8701 cacatggctc tcagtaagca atggtgcatt tgccaaaaaa gaagtagccg atagtgccca
     8761 aactttgatt cgttgatctc accacaggct tatgagatcc tcagtcaaat actctatccc
     8821 caaagttgtc aaaagacact tgaaggaaag cgtcattttg accggcccaa gagtcctagt
     8881 ccatccgaca gttgagagtg tagtttttgc cccatactaa aactcacctt tctgatgaac
     8941 tcaaaaacgg tcggccttcc acagttaact aggcagccta tggaaactca taaaccatga
     9001 attcagtttt tccccataat cagagcctca agagcacctg tccttttgat atcaacaaca
     9061 tataggagat acttcatgag taaacaatcc attcgtttct tatccatcca aattggctta
     9121 gcaatacttc tagctgtgcc agtgtttggc gacaccagtc ctttacctgc ctggaaggaa
     9181 ggggccacta aatcagccat tctgcagttt gtcgccaaag ttacggaaga aggcagtccg
     9241 g
//
LOCUS       DAORAQ010000104         9119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1201_length_9119_cov_61.114078, whole genome shotgun sequence.
ACCESSION   DAORAQ010000104 DAORAQ010000000
VERSION     DAORAQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1201_length_9119_cov_61.114078"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            498..1070
                     /locus_tag="PKM72_09615"
     CDS             498..1070
                     /locus_tag="PKM72_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNP61086.1"
                     /translation="MIQIRIHGRGGQGVVTAAELLALTAFRDGKEAQAFPSFGSERTG
                     APVTAFCRIDSQPIRSREPVYHPDVLLIQDSTLLHQVDVFAGLAATAFVLINSTRHVD
                     VLHLTDFVTQHPGVQMHTLPASDLALKHLGRPFANIGMVAGYAALTGEVTQASVKQAI
                     TGKFPGTIGKLNAAVAEEVYAYVAANLATV"
     gene            1097..2350
                     /gene="porA"
                     /locus_tag="PKM72_09620"
     CDS             1097..2350
                     /gene="porA"
                     /locus_tag="PKM72_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNP61087.1"
                     /translation="MKQHIEGSQAVAHAVALCRPEVMACYPISPQTHIVETLSRKVKA
                     GEIGNCQFLNVESEFGALSVLIGASAMGARTYTATTSQGLLFMAEAVYNAAGLGLPIV
                     MTIANRAIGAPINIWNDHSDSMAMRDAGWIQLFAEDNQEAVDLHIQAFRLAEELSCPV
                     MVCMDGYILTHAYEVVDLPTQEQVDAYLPPFEPVQVLDPQDPVSIGAMVGPEAFAEVR
                     YLAHDKHLRALEVIPRLSQTFQDIFQRRSGGLLKTYHTDEAETILLALGSVNGTIKDA
                     VDDLRKEGWPVGSIALRAFRPFPSHALRHVVKDAKRIIVIEKDLAVGKGGIVSSDVKM
                     ALQGLPIIVDTVIAGLGGRPIPKASVIDTVKQACLEGLEEPHFLDLNWDAINGELTRQ
                     QAQRRSGSTAENILREIGAPGASPI"
     gene            2371..3366
                     /locus_tag="PKM72_09625"
     CDS             2371..3366
                     /locus_tag="PKM72_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNP61088.1"
                     /translation="MSYQRVKFYQTGTFTVGNRLLDESDRTVQAGMDRTNSLNSGHRA
                     CQGCGEALGARYALDAVMRATHQQMVAVNATGCLEVFSSPYPESAWQIPWLHSLFGNA
                     PAVASGVAAALRAKGRTDIRVIAQGGDGATVDIGFGCLSGMFERNDDVLYVCYDNEAY
                     MNTGVQRSGSTPPRAWTNTTQAVGTDPGNPMGIGKNLPRIAMAHGIPYVATASVADLH
                     DLEAKVTKAMSIRGARYIHIHVPCPLGWKSDPAHTIKVARLAVESGLFPLFEAEGGEV
                     TDRTPIRKKVSVEQYLELQGRFRHLFHPRDEQSLGAIQAMADANIDRYQLLPSEA"
     gene            3363..4997
                     /locus_tag="PKM72_09630"
     CDS             3363..4997
                     /locus_tag="PKM72_09630"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNP61089.1"
                     /translation="MMMEQKPFAITLDPASSLANHTGNWRTMRPVYVDHLPPCNQGCP
                     AGENIQGWLYEAEEGKYEQAWRKIMEDNPFPAIHGRVCYHPCETACNRGQLDEPVSIH
                     AVERFLGDYAIEQGWQVEAGVPTGKRVLVVGAGPSGLSAAYHLVRLGHAVTVIEAGPK
                     SGGMMRFGIPQYRLPRTIVDAEIARIVAMGVSVHLNHKVEDLETTIQGGGFDAVFLAI
                     GAHLSKRVDIPGRDAGRMIDALGFLKEMDGEAIPKIGRRVVVYGGGDTAIDAARTAKR
                     LGAEETILIYRRDRRHMPAHDFEVEEALEEGVVTRWLNTIRQVNDTTLTVEEMTLDEQ
                     GHPQPTGRIETLEADTVILAVGQEVDTGFLEKVPGLDISSDGVIQVDASMMTGRAGVF
                     AGGDMVPAERTVTVATGHGKKAARHIDAYLRGGEYRKSPPNAVATYDRLNTWYYTDAD
                     KSRQPYLDLARRRAGFDEVVGGLDKDTALLESRRCLSCGNCFECDTCYGVCPDNAIIK
                     LGPGQRYEVNYDYCKGCGVCAEECPCGAIDMVKEVR"
     gene            5109..5975
                     /locus_tag="PKM72_09635"
     CDS             5109..5975
                     /locus_tag="PKM72_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61090.1"
                     /translation="MKYLLGIDGSDQSVDATRVFEALSPAESLMVLYVVNVPGIPYPA
                     MGAGVAKDLSMAVDKAMREEGERAIDQAVSLLPLNPGPVKKRLETGKPAEVILTLAEE
                     QNVDLVVLGARGIGQIREQMFGSVSHRVMTHAPCSTLIIKRPVRKIQQVCIPVESQED
                     GEAVVRFMKKKPFRVPISAIVLHVIPFSEPIWPVGAMISPEFRNEMIAHGEKFTNDLS
                     RELKQLGHDAQGVVVVGAPSQTIIEEATKQAADVILMRTHSRSGVSRFLLGSVSHSVV
                     HHTESSILFVRE"
     gene            complement(6069..6287)
                     /locus_tag="PKM72_09640"
     CDS             complement(6069..6287)
                     /locus_tag="PKM72_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61091.1"
                     /translation="MKRAKVRHDTKAMPTVITDQGARSTYSGSAGRQGRTFEHKKFNK
                     RIQEEVRLQMKKALLTGAGTPEDQIEKN"
     gene            6520..7203
                     /locus_tag="PKM72_09645"
     CDS             6520..7203
                     /locus_tag="PKM72_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61092.1"
                     /translation="MGNVTDRKGIFRLLGLAVTISLVFATSLGASPSVQQISHLIIPI
                     LGTSVNKQFRPVGMVSQVVIDVYERQDRNGLQIQFHTEPGKFSLFARQSIHAAITRAL
                     AAANLPSDSWTVLLKFPYSGQTIYGESLSAMVGLSVVSMIKGDHLLEGRALTGTITEG
                     GFIGAVGGLQLKILAAYNNHFQRVLVPSEYDVRDGDWRTPFLMHVSPVGTVDEAYFGL
                     TGHHLATLP"
     gene            complement(7295..8611)
                     /locus_tag="PKM72_09650"
     CDS             complement(7295..8611)
                     /locus_tag="PKM72_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNP61093.1"
                     /translation="MPGTPDKSRTIKIDMMARVEGEGALHITVQNGQVRDVQLRIFEP
                     PRFFEAFLQGRHVNEVPDIVARICGICPVAYQMSAVHALEQIFGVEMPQSLRDLRRLL
                     YCGEWIESHVLHIYMLQGPDFLGFDSGLEMARSHPDLVKRGLRMKKAGNQLLSLLGGR
                     SVHPVSVQIGGFSQVPTRKALHALKEEFLWARDAATETLRWVSRFEFQDPEQAYTFLA
                     LRGPSEYPMNEGDIVTNRGHHMAPSEFEQHFHEEQVPYSTALHCTLNGQSYLTGPLAR
                     LALNQDQLSLLPKDVLQSTGLVLPFNRPSMGIVARSVEVLYAFDEALRLMNQYEPPDV
                     PAVPVALRAGMGMAATEAPRGLLYHRYEVNADGTIRIAKIVPPTSQNQRRMEDDLRKL
                     LPDILHLTDQETAMKCEQLIRSYDPCISCATHFLTLTKEDLPMTMP"
     gene            complement(8595..>9119)
                     /locus_tag="PKM72_09655"
     CDS             complement(8595..>9119)
                     /locus_tag="PKM72_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61094.1"
                     /translation="IQALRNWGDVEAFKRTVYPSPEYIHSLSTSTPISEHVQVDYELW
                     GCPIDKQQLLRVLTDLLRGVSPSIPTHSVCLDCKRQGHVCVLVAKGLPCLGPVTRTGC
                     GAICPAMGRECYGCFGPAEQDSVIPGVPPNTRSLANQFHKHLQLIPVEIVRRFRGING
                     SVQPFQKESDAWDS"
BASE COUNT         2070 a         2232 c         2619 g         2198 t
ORIGIN      
        1 cgtaagagtt gtcgcatttc cgccagaata aagtcgcgtt ctgctggagc cagaagcacg
       61 gccgttcggc catctgcgga gggggtggtc tctcctttca caaacaacca gcccagcacg
      121 ccggcgctga ctatccagag cacgagagta atcctgcaaa tattttttga gaaggatgat
      181 agaaatgaca acgggggcct ccttattttt ccaataactt gttgatccga tttttgtgga
      241 catctgtctt gctgctttca cgagtcgatt tcacttagga acttgactgg ggcatttttc
      301 tacatcaccc acgattttga ctgtacctct tttccacaaa aatccggctc attgatccgg
      361 gtctgctcaa gattactgag gttttgctgg ttgaatctaa catttccatc tctcatcaaa
      421 taaggcatat ctcttgccaa atacatcaag agtgagtgaa cgacataaac ctattcaggt
      481 gaattgatga gggacgaatg atccaaatcc gtattcatgg cagaggaggg caaggcgtgg
      541 tgacggctgc cgagctgctg gcattaacgg cattccggga tgggaaagag gcgcaggcct
      601 ttcccagttt tggatctgaa cggaccggtg ccccggtcac ggcattctgc cggatcgatt
      661 ctcaaccgat acgcagtcgg gagccggtct atcatccgga tgtcttgctc atccaggact
      721 ccactctgct gcatcaggtg gacgtctttg ccggattagc tgcaacagcc ttcgttctga
      781 ttaattccac acgacatgtg gatgtgcttc atctcactga ctttgttacc caacatcccg
      841 gagtgcaaat gcatacgctc cctgcatccg atctggctct gaagcatctg gggcgaccat
      901 ttgccaatat cgggatggta gccggctatg cggccctgac gggggaagtc actcaagcct
      961 ccgtgaagca ggcgattaca gggaagtttc ctggaacgat tggaaaattg aatgcggcag
     1021 tggcggaaga ggtctatgcc tatgttgcgg ccaatctggc cacggtctaa ttcaatcggg
     1081 gaatcaacag aagaccatga aacaacacat tgaaggatct caagccgtgg cccacgctgt
     1141 cgccctctgt cggccggaag tgatggcttg ttatccgatt tctccgcaaa cccatattgt
     1201 cgaaacgctt tcgcgcaagg tcaaagcggg agaaatcggc aattgccaat ttctgaatgt
     1261 tgaatcagag tttggcgcgt taagcgtgct gatcggcgcc tcggcgatgg gtgcgcgaac
     1321 ctataccgcg acgaccagtc agggactctt gtttatggcc gaagcggtct ataacgcggc
     1381 gggcctggga cttcccattg tcatgacgat agccaatcgc gctattggtg cccctattaa
     1441 tatctggaac gatcattcag acagcatggc catgcgagat gccggctgga ttcaattgtt
     1501 tgcggaagat aatcaagaag ctgtcgacct tcacattcaa gcgtttcgcc tcgcggagga
     1561 actgagttgt ccggtgatgg tctgcatgga tggttatatt ctcacccatg cgtatgaggt
     1621 ggtggatctc cctacgcaag agcaggtgga tgcctacctc cccccgtttg aaccggttca
     1681 agtgttagac ccgcaggacc ctgtgtccat aggcgcgatg gtcgggccgg aagcctttgc
     1741 ggaggttcgg tacctcgcgc atgacaaaca tcttcgagcg ttagaggtga ttccacgact
     1801 ctctcagact tttcaggata tcttccaacg tcgctctggt gggctgttga agacttatca
     1861 caccgatgag gctgaaacga ttctgcttgc actgggatcg gtgaacggaa ccataaaaga
     1921 tgcggtggat gatttgcgga aagaaggatg gccggtgggt tcaattgcgc tccgtgcctt
     1981 tcgtcccttt ccctcccatg cgctccgtca cgtcgtcaag gatgccaagc ggataattgt
     2041 cattgaaaaa gacctggcgg taggaaaggg cggcattgtg tccagcgatg tgaagatggc
     2101 gcttcagggg ctaccgatca tcgtggatac agtcatcgcc ggacttggag gtcggccgat
     2161 tcctaaagcc tcggtcattg atacggtgaa gcaagcctgt ctggagggtt tggaggaacc
     2221 ccatttcctc gatctgaatt gggacgccat taatggggaa ttgaccaggc aacaggcgca
     2281 gcggcgttcg ggatccaccg cggaaaatat tcttcgggaa atcggcgctc ccggcgcatc
     2341 gcccatttaa tttttgatgg tgaggccgct atgtcctatc aacgtgtgaa attttatcaa
     2401 acagggacct tcacggttgg caaccggtta ctggatgaat ccgatcgcac ggtgcaagcc
     2461 gggatggatc gaaccaattc actcaattcg ggtcatcgtg cctgtcaggg gtgcggagaa
     2521 gcactgggtg cccgttatgc gcttgatgcg gtgatgcgtg ccacacatca gcagatggtc
     2581 gcggtgaatg ccaccggctg tttggaagtc ttctccagtc cctatccgga aagtgcctgg
     2641 cagattccct ggctgcattc gttgttcggg aatgcccccg cggtggcgag cggcgtggcg
     2701 gcagccctcc gggccaaggg cagaacggat attcgtgtca ttgcccaggg cggagatggc
     2761 gcgacggttg atatcgggtt cgggtgtttg tccgggatgt tcgaacgcaa tgatgacgtg
     2821 ttgtatgttt gttacgacaa tgaggcctac atgaacacgg gagttcagcg ttcggggtct
     2881 accccgccac gagcctggac caataccacg caggccgtgg gaacagaccc cggcaatccc
     2941 atgggcattg gaaaaaatct tccacgaatt gcaatggctc acggtattcc ctatgtggcc
     3001 acagcctccg tggccgatct ccatgatctg gaagccaaag tgacgaaggc catgagcatc
     3061 aggggcgcac ggtatattca tatccatgtt ccctgcccgc ttggttggaa atcggatcct
     3121 gcgcatacga tcaaggttgc ccgcctggca gtggaaagcg gcctgtttcc tttgtttgaa
     3181 gcggaagggg gcgaagtgac tgatcgaaca ccgatccgga aaaaagtttc tgtcgaacaa
     3241 tacctcgaac ttcaaggtcg cttcagacat ctctttcacc cacgagacga gcaaagtctt
     3301 ggagcgattc aggcgatggc ggatgcgaat attgatcggt atcagctact tccttcggag
     3361 gcatgatgat ggaacagaag cccttcgcca ttacattgga ccctgcttca agcctggcta
     3421 atcataccgg caactggcgg acgatgcgtc cggtctatgt tgatcacctt ccgccctgca
     3481 atcagggttg cccggctggc gaaaatattc aaggctggtt gtatgaggcg gaggaaggga
     3541 aatacgaaca ggcctggcgg aaaattatgg aagataatcc cttcccggcg attcatggtc
     3601 gcgtgtgtta tcacccgtgt gaaacggctt gcaatcgggg ccaactcgat gagcccgtga
     3661 gcattcatgc cgttgagcgg tttttaggcg attacgcgat tgagcagggg tggcaggtgg
     3721 aggcgggagt gcccaccgga aagcgggttc tggtcgtcgg cgcgggaccc agtgggctat
     3781 ctgccgccta tcatctcgta cgtctgggtc atgcggtcac ggtgattgaa gccggtccga
     3841 aatcgggagg catgatgcga ttcgggattc cacaataccg tctccctcgc acgatcgttg
     3901 atgctgaaat tgccagaatt gtcgccatgg gcgtctcagt ccatctgaat cacaaggttg
     3961 aggatttaga gacgaccatt caaggagggg gattcgatgc cgtgtttctg gccattggcg
     4021 ctcatctgag taagcgggtt gatattcccg gtcgggatgc cggccggatg atcgatgcct
     4081 tgggatttct caaggaaatg gatggtgagg ctattcccaa gatcggtcgg cgggtcgtcg
     4141 tctatggtgg aggtgatacg gccattgatg ctgcgcgaac ggccaaacga ctaggtgcgg
     4201 aggaaaccat cctgatttat cggcgtgatc gcaggcatat gcccgcccat gactttgaag
     4261 tggaagaagc gttggaggag ggtgtggtga cacgatggct gaacaccatc agacaggtga
     4321 atgatacgac attgaccgta gaagaaatga cgctggatga gcaggggcac cctcaaccca
     4381 cgggccggat tgagaccttg gaagctgaca cggttattct ggcggtgggg caggaagtgg
     4441 ataccgggtt tttagagaaa gtgccgggat tggatatttc gtccgatggc gtgatccaag
     4501 ttgatgcctc gatgatgacc ggccgtgcgg gagtgtttgc cggaggggat atggttcctg
     4561 cagagcgcac ggtgacggtg gccaccggtc atgggaaaaa agcggcacgg catattgatg
     4621 cctatctccg tgggggggag taccggaaat ccccgcccaa tgccgttgcc acgtatgatc
     4681 gactcaatac ctggtattac accgatgcgg acaaaagtcg gcagccctac ctggatttgg
     4741 ccaggcgtcg ggccggcttt gatgaggtgg tgggtggatt ggataaggac acggcgttgc
     4801 tcgaatcccg gcgatgtctc tcttgcggga attgctttga gtgcgatacc tgctatggcg
     4861 tgtgtccgga caacgccatt atcaaactgg gacctggtca acgatatgaa gtgaattatg
     4921 actattgtaa aggctgcggg gtgtgtgcgg aggaatgtcc ctgcggcgct atcgatatgg
     4981 tcaaagaagt cagataggaa caagaaaatc gtgcgcacgg cagaggcagt cgttggaaaa
     5041 agtacaatga gtcatttggc atcaacacat acggcacaaa aaatgtgtca cacccgggag
     5101 atgtacctat gaaataccta ttgggcattg atggatcgga tcaatccgta gatgccacac
     5161 gagtgtttga agccctcagc ccggcagaaa gcttgatggt cttgtatgtg gtgaatgtgc
     5221 cgggaattcc ctatccggcc atgggtgcgg gtgtggcgaa agatttgtcg atggcggtgg
     5281 ataaagcgat gagggaagaa ggcgaacgcg ctatcgacca agcggtgtcc ctcctgcctt
     5341 taaatccggg acccgtgaaa aaacgactgg aaaccggtaa gcccgccgag gtgattctta
     5401 ccctggcgga agagcagaac gttgacctgg tggtgctggg cgccagggga atcggtcaga
     5461 tacgagaaca gatgttcggg agtgtctcgc atcgggtcat gacccacgcg ccgtgttcga
     5521 ccttgatcat taagaggccc gttcgcaaga tccaacaggt gtgcatcccc gttgaaagcc
     5581 aggaggacgg tgaagcggtc gtgcgattca tgaaaaagaa gccctttcgg gtaccgatct
     5641 ctgcgattgt tctgcatgtg attccatttt cagaaccgat ctggcctgtg ggggcgatga
     5701 tttctccgga atttcgaaat gagatgatcg cccatggaga gaaattcacg aatgatctca
     5761 gcagggaatt aaagcagctg ggacatgacg cccaaggggt cgtcgtcgtc ggggcaccct
     5821 cacagacgat cattgaagaa gcgaccaagc aagcggctga tgtcatcctg atgcgaacgc
     5881 atagccgatc aggggtgagt cgatttcttt tggggagcgt ctctcattcc gttgtgcatc
     5941 acacagagtc ttccatcttg tttgtacgcg aataacccgg tctaaaggac aaatctggat
     6001 aaactatctt ttccccgtgg cctatgaata aaacctggtt tcttattggt gccgttgcgt
     6061 taggagtatt agtttttctc tatctgatct tctggtgtcc cagcccctgt cagtaaggct
     6121 tttttcattt gcaggcgcac ctcttcctgg atccgtttgt taaatttttt gtgttcgaat
     6181 gtccggcctt gccggcctgc gctccccgaa taagtcgacc tggcaccctg gtcagtgata
     6241 acagtgggca tcgctttggt gtcatgccga acttttgccc ttttcaagac acattccaga
     6301 caaatctgtt gctggaacta ggggttactg agttctttga cataatgtat gggacatttc
     6361 aggcaaaaat tcgaagacgt ttggtaagga ataaaggggg ccttatgagg agtggagcca
     6421 tccaggccct cataagccga caggcgacaa aaaaattgcc gttcggtata atgatatcaa
     6481 gggaggtaag ttccctcttt cattgaggga tatctaaaga tgggcaatgt gactgacaga
     6541 aaggggatct tcaggctact tggattggcc gtaaccataa gtttggtgtt tgccaccagt
     6601 ctaggagcca gtccgtctgt tcagcagata tctcatttaa tcattccaat ccttgggaca
     6661 tctgtcaaca aacaattcag gccggtaggc atggtaagcc aagtggtgat tgatgtgtat
     6721 gaacgccagg atcgcaacgg ccttcaaatc caattccata ccgaaccagg aaaattttcc
     6781 ttgtttgctc gtcagtcgat tcatgcagcc atcacccggg cgctggccgc cgccaatcta
     6841 ccgtctgatt cctggactgt cctcttgaaa tttccctatt ccggtcagac gatctatggg
     6901 gagagtcttt cggccatggt gggtctgagt gtggtatcca tgatcaaagg agatcatctc
     6961 cttgagggtc gagcccttac tggaacgatc acggaaggag gattcatcgg agccgttggg
     7021 ggacttcaac tcaagatttt agctgcttac aataaccact ttcagcgtgt gttggttccc
     7081 agcgagtatg atgtacgcga tggagactgg cgtacccctt ttctgatgca tgtctctcct
     7141 gtggggacgg tcgatgaagc gtactttggc ctgaccggcc accatttggc gacccttccg
     7201 taaggcctgc ctttcagtct tctcgcagtt gctccggcat aatggttgat tatggtttcc
     7261 tgcccggttt ctctatcgta attcccggtg tgatttatgg catcgtcata ggtagatctt
     7321 ctttcgtcaa cgttaaaaaa tgtgtggcgc aggagatgca gggatcataa ctgcgaatca
     7381 gttgttcgca tttcatcgct gtctcctggt cggtgagatg gagaatgtca ggtagcagtt
     7441 ttcgaagatc gtcctccatg cggcgctggt tttgggacgt tggtggcacg atcttggcga
     7501 tacggatggt tccatcagca ttgacctcat agcgatgata cagcaggcct ctgggggctt
     7561 cggtggcggc catacccatt ccggcacgaa gcgctaccgg aacggcagga acatctggcg
     7621 gttcatactg gttcatgagg cgaagggctt cgtcaaaggc atagagtact tccaccgatc
     7681 gtgccacgat acccatggat gggcggttga aagggaggac taatcccgtg ctctgtaaga
     7741 cgtctttggg caacaacgag agctggtcct ggttgagcgc aagccgggct aacggccctg
     7801 tgagataaga ttgtccattc aacgtgcagt gaagggcagt ggagtagggc acctgttctt
     7861 catggaaatg ctgttcaaac tccgatgggg ccatgtggtg ccctcgattg gtgacgatgt
     7921 cgccttcatt catcggatat tcagaaggtc cacgaagcgc gaggaaggta taggcctgtt
     7981 cgggatcttg aaattcaaac ctgcttaccc atcggagcgt ttccgtagcg gcatctctgg
     8041 cccaaagaaa ttcctctttc aaggcatgca acgcctttct ggtcggcacc tgagaaaagc
     8101 cgccaatttg gaccgagacc ggatggaccg accgtcctcc taagagcgac aaaagctgat
     8161 tgcctgcctt cttcattcgc agacctcgct tcacaaggtc gggatgggaa cgagccatct
     8221 caagcccgct gtcgaatcct aagaagtcgg gaccctggag catatagatg tgcaggacat
     8281 gactttcaat ccattccccg caatacagga gtcgacgtag atctcggaga ctttgaggca
     8341 tctccactcc gaaaatttgt tccagggcat ggaccgcact catttgatag gccaccggac
     8401 agataccgca tatacgcgcc acgatatcgg gcacttcgtt gacgtgacgt ccttggagga
     8461 aggcttcaaa aaatcgagga ggttcaaaga tccggagttg cacatcccgg acctgaccat
     8521 tctggacggt gatgtgaagg gctccttccc cttctaccct ggccatcatg tcgattttga
     8581 tggttctcga tttatcagga gtcccaggca tcgctttcct tctgaaatgg ttgaactgat
     8641 ccgttgattc ctcgaaatcg gcgaactatt tccacgggaa tgagttggag atgtttatga
     8701 aattggttgg caagggatcg ggtatttggg ggtactccgg gaatcactga atcctgctcg
     8761 gccgggccaa agcacccata gcactcccgg cccatagcgg ggcagatggc tccgcacccg
     8821 gttcgggtca cgggtccaag acatggcagt cccttcgcga ccaggacgca gacatgcccc
     8881 tggcgtttac agtccagaca aacactatgt gtgggaatgc tgggtgagac gcctcttagt
     8941 aaatcggtga ggactcgaag gagttgttgc ttatcgatgg ggcatcccca caattcatag
     9001 tccacctgga cgtgttccga aataggagtc gaggtgctga ggctatgaat gtattcgggg
     9061 ctgggataca ccgttcgctt gaaagcctcc acatcgcccc agttccgaag agcctgaat
//
LOCUS       DAORAQ010000105         9042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1217_length_9042_cov_78.526427, whole genome shotgun sequence.
ACCESSION   DAORAQ010000105 DAORAQ010000000
VERSION     DAORAQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1217_length_9042_cov_78.526427"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1826)
                     /gene="arc"
                     /locus_tag="PKM72_09660"
     CDS             complement(144..1826)
                     /gene="arc"
                     /locus_tag="PKM72_09660"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HNP61095.1"
                     /translation="MGIRKSDDHIREIEKLRTEIESMELEMRQLHESRQKLHLSQQKN
                     EQLVSTLQEAKAQIEALRKEVDKLTAPPSTFAIFYNTNADGTVTVSLGGRKLRVNLHP
                     SIPADSLKKGQEVILNEGLNVIESRGYDPQGEIAHLKHLLDDGRAVVSLRLDDERVVE
                     ISDSLKQQTLSVGDHLLFEPRSGYLIEKLPKGEMEELVIEEVPDIQYDQIGGLTKELE
                     QVKDAVELPFLYPELFAEHRLSPPKGLLLYGPPGCGKTLIAKAVANSIAKKLGHLKGK
                     DVRSYFLHIKGPELLNKYVGESERQIREVFAKAKEKASHGNPVIVFFDEMDALFRTRG
                     TGISSDMESTIVPQFLSEIDGVESLRDVIVIGASNRQDLIDPAVLRPGRLDIKIKIPR
                     PDKQSAKDILGKYLTSDLPLAEAELTQHGSDRSTFINHLIETTVEAMYALTEENQFLE
                     VTYANGEKETVYFKDFSSGALIESVVSRAKKLAIKRAISGEAKGLKPEDFLRGIREEF
                     KEQEDLPNTTHPDDWAKIAGKKGEKIVHVRTLTTDEDSEPREIETISVGHYF"
     gene            complement(2019..2645)
                     /gene="thiE"
                     /locus_tag="PKM72_09665"
     CDS             complement(2019..2645)
                     /gene="thiE"
                     /locus_tag="PKM72_09665"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNP61096.1"
                     /translation="MSSRILPKLYLLTDRHQTLNRPLSSVITEAVDAGVRMVQVREKD
                     LKTRDLTSLCQQLFPLIKHQHGTILLNDRIDLVLALGADGVHLRTNSLPVSVARRLLG
                     TGHLIGVSTHSVEEAQVAEAEGADFIVLGPIFDTPSKRAYGPPLGIQILQKTSRTIHV
                     PIYAIGGITPIRVPDVLSSGAYGVAVISSILQSPSIPDITGELLARLS"
     gene            complement(2659..3432)
                     /locus_tag="PKM72_09670"
     CDS             complement(2659..3432)
                     /locus_tag="PKM72_09670"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNP61097.1"
                     /translation="MNQDPLIIAGRTFRSRLWVGTGKYQNFEETRKAIEASGADVVTV
                     AVRRVNITDHKSENLLDYLDPKKYTILPNTAGCYTVEDAVRYSRLARAAGVSDLVKLE
                     VIGDERTLFPDTAGLIEAAKILIAEGFVVLPYTNDDPIVAKKLADIGCPAVMPLAAPI
                     GSGLGIRNPYNLKIIMETVNVPVIVDAGVGTASDAALAMEYGADAVLMNTAIAGAKDP
                     LMMATAMRYAVDAGRLAYRAGRIPRKLYATASSPIEGML"
     gene            complement(3452..3649)
                     /gene="thiS"
                     /locus_tag="PKM72_09675"
     CDS             complement(3452..3649)
                     /gene="thiS"
                     /locus_tag="PKM72_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNP61098.1"
                     /translation="MNGEQREAEEPLTVAQLLSTLDLRSEQVAVEINLKILDRGDFLT
                     WNLHDGDKVEILSFIGGGCPT"
     gene            3905..4381
                     /locus_tag="PKM72_09680"
     CDS             3905..4381
                     /locus_tag="PKM72_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNP61099.1"
                     /translation="MEKNINQDLMALFLSMAGLLASRGENPYKVKAYRRAADSLKSLQ
                     EDVTEVANRGELQTIPGIGKELSIKIQEFLSTGRIRAYEELKTPLPDSALDWVNLPGF
                     SEPIVHDLVFRLGITSWEDLEALAQSHLLQTLPGLGTRGEEILTAIRKKRALCQES"
     gene            4504..4740
                     /locus_tag="PKM72_09685"
     CDS             4504..4740
                     /locus_tag="PKM72_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNP61100.1"
                     /translation="MAISAFVLIDTTGNHTKSAYKTLTRIQGVKSVYPVTGPFDLIAH
                     VEAETLEELNDLIMVRLRGIDGVIKTNTAIVLDF"
     gene            complement(4768..6081)
                     /locus_tag="PKM72_09690"
     CDS             complement(4768..6081)
                     /locus_tag="PKM72_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNP61101.1"
                     /translation="MSHERLSTVVISGLTLTAILLVGIVIGKGGDRAGYASEPYEELQ
                     VFAEVLSQVKKHYVEETQTKDLVQGALRGMLAGLDPHSSFMTPDMFKEMQVETKGEFG
                     GLGIQIGVKNNRLTVISPIEDTPAFEAGIQPGDTIIKVDDKPTKDLTLMEAVQHMRGA
                     RGTSVELTIEREGLEKPLIFNLKRDIIKIQSIRSKLLEGNLGYVRLSQFQEATVEDLS
                     TELEKLVAKNIQGLILDLRNNPGGLLTAAVGVSEQFLESGRLVVSIQGRNGKKDEYRA
                     RASSKNYQYPMIVLVNHGSASASEIVAAAMQDWGKAVVIGTTTFGKGSVQTILPLSDG
                     SGLRLTTAKYFTPNGKSIHSIGVQPDIVIDPKPAQVAKQESSEGKKDESTADSPAPSP
                     TKPQAPSAPQDKDQAQPIPPDDLQLLKAVEMLKSWKVFKQLKPAA"
     gene            complement(6109..7302)
                     /locus_tag="PKM72_09695"
     CDS             complement(6109..7302)
                     /locus_tag="PKM72_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNP61102.1"
                     /translation="MRRVKLFLHTGLTSGLLLLFVQAGWGADPSTIKNEISQERLRLE
                     KLNQEITETKKETKRIEKKHGSVLKTIDKLDRQLHEQKKEHNRINDQIKATDEELANL
                     STKITEVEQDVRTRQSSIAARLRLLYVEGRTGYLKTLFAAESFTEASNRMNYLSWVAQ
                     REHQLVRQFQEDLSNLQQLKEKQTAAREDLLRFQGETQQTIKKVSGLKREKRSVLVNL
                     SKEKDAHQRMVEDLQRSAEQVDSLLKELDQRFQLAQSQMRKPSGAPPSLGSFIWPVEG
                     KVVSNFGRQKHPTFDTFVTKKGIEISVQEGSPIRAVSSGNVVYADWLKGYGLVIIVDH
                     TNGFYSLYAHASKLLVAEGHSVKNGQVIGETGETGVTQEDTLYFELRKGTTPIDPLKW
                     LVKQP"
     gene            complement(7299..8198)
                     /locus_tag="PKM72_09700"
     CDS             complement(7299..8198)
                     /locus_tag="PKM72_09700"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP61103.1"
                     /translation="MKRLWYFIREAYTSLRTHRTSTLIGVLTTAFTLTSFGIFLLLYH
                     NVHNLLGQVQHTIQIIVYPKNDIESGKLHDLQQGLKTDPAVDSLTYISKQDALTEFKE
                     QFPEEVYLLEGLGESPFPASLILTMAAKSPTTDMIGNLVNRLQHHPDIDRVRYNRDWI
                     ERLTLIIAYMEFGALLVGSILGLASVTIIAITVQLAFHTRQQEIEILRLIGATSLFIS
                     IPYLIEGAILGAAGGGMALGFLRGGFELFTQNTQGLNVFGGFSSALEFFPLPLSLILI
                     AGGILLGCLGTLTTIYGSLRFRL"
     gene            complement(8195..8863)
                     /gene="ftsE"
                     /locus_tag="PKM72_09705"
     CDS             complement(8195..8863)
                     /gene="ftsE"
                     /locus_tag="PKM72_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNP61104.1"
                     /translation="MIQLFHVTKYYDGCLALSDISLRIEKGECVLLTGPSGAGKTTLL
                     KLIFGAEHPDEGQILVQNRNIARLGGSDIPYHRRTMGYVLQDFKLLPQRTVSENVALP
                     LIIRGLSSFESRRKVLEALRAVGMDHKLNSIASVLSAGEQQRVCIARAIVNSPIILLA
                     DEPTGNLDPQLTTEIAALFKAINTRGTTVMMATHNRQVVEQLNGRILRLDCGHLVSDQ
                     GVYT"
BASE COUNT         2168 a         2091 c         2303 g         2480 t
ORIGIN      
        1 gggagatggg gacagtgact gattaaccga agggaattcg ctacgggatc agggtggcct
       61 gtggagcgag aaataattcc gaattcagtt tccgttccaa gaattcgcag caaggaggcc
      121 tcatggagag cagctaaaaa tggttaaaaa taatgcccaa cgctgatggt ttcaatttct
      181 cgaggctctg aatcctcgtc ggtggttaag gtgcgcacat gaacgatttt ttcgcctttt
      241 ttgcctgcta tttttgccca gtcatcgggg tgagtggtat ttggtaaatc ttcctgttct
      301 ttgaattctt cccgaatgcc ccgcagaaag tcctcgggtt tcaggccttt ggcttcgccg
      361 gaaattgccc gtttgatggc cagttttttg gcacgggaga ccacactttc gatcaacgcg
      421 ccactggaaa aatctttgaa gtacaccgtt tccttttccc cattggcata ggtgacttcc
      481 agaaattgat tttcttccgt caaggcatac atggcctcca cggtggtttc aataagatga
      541 ttgataaacg tcgatcgatc tgagccatgt tgggtcaatt cagcttccgc aaggggcaag
      601 tctgaggtca gatatttccc gagaatatct tttgcactct gcttgtcagg tctgggaatc
      661 ttaattttaa tatccagacg ccccggtctc aaaaccgccg gatcaatgag atcttgccgg
      721 ttactggcac cgatgacgat gacgtctcga aggctttcta ctccatcaat ttcggataag
      781 aattggggaa cgatagttga ttccatatcc gaggaaatac ctgtccctct tgttcggaat
      841 aacgcatcca tttcatcaaa gaagacaatg accggattgc cgtgtgacgc cttctccttt
      901 gctttcgcaa acacttctcg aatttgacgt tcggattcac cgacatattt gttcaataac
      961 tcgggtccct tgatgtggag gaagtaactg cgaacgtcct tgcctttcag gtgaccgagt
     1021 ttttttgcga tggagtttgc gacagctttg gcaatcaagg ttttcccgca tcccggcggt
     1081 ccatatagca aaagaccttt gggaggtgat aaacggtgtt cagcaaatag ctctgggtac
     1141 aggaagggca attccacggc atccttcacc tgctccagtt ctttggttag cccaccgatt
     1201 tggtcatact gaatatccgg aacttcctcg atgacgagtt cctccatttc acctttggga
     1261 agtttttcga tgaggtatcc ggatcgtggt tcaaagagga ggtggtcgcc gacactcaaa
     1321 gtctgctgtt tcagcgaatc agagatttca accactcgct cgtcgtccag gcgaaggctg
     1381 acaactgctc gcccatcgtc caggaggtgt ttgagatggg cgatttcgcc ctgcggatca
     1441 tacccgcgcg attcaatcac gttcaggcct tcgttcaaaa taacttcttg gcctttttta
     1501 agggaatctg ccgggataga tggatgaagg ttgacccgta gctttctacc gccaagtgag
     1561 acagtcacgg tcccgtctgc gttcgtgttg tagaagatgg caaaagttga tggaggcgcc
     1621 gtgagtttgt cgacttcttt tcgaagcgct tcgatttgtg ctttggcttc ctgtaacgta
     1681 gaaactaact gctcgttttt ctgctgagaa aggtgcagct tttgacgaga ttcatgcagt
     1741 tgccgcattt cgagttccat ggactcaatc tcggttcgta acttttcaat ttcacggata
     1801 tgatcgtctg attttcggat tcccattgtc ggatcctatc tggttcgagg tttcaaagaa
     1861 accggaagac acatattgct gagaagaacc cctgccttgt ttgaatgccg gggaaagagg
     1921 atgtccgcac catccttcac tgtgaagatc ttaccaccca aaaattttta gggtcaaccg
     1981 aatacgggca ttatgactgc ctgaaataat ttgacaattc aggataatcg tgcaagcagt
     2041 tcccctgtga tgtctggaat tgagggcgat tgaagaatgg aagaaatgac ggcgactccg
     2101 taagccccgg atgataagac gtcaggaact ctgatcgggg tgatacctcc aatggcatag
     2161 atgggcacgt ggatagttcg gctcgttttc tgaagaatct ggattcctaa aggtggtcca
     2221 tatgctcgct ttgaaggcgt atcaaaaatg ggaccaagca cgatgaagtc ggccccttcc
     2281 gcttccgcca cttgggcttc ttcaacggaa tgcgtcgaga cgccgatgag gtgtccggtt
     2341 cctaacaggc gacgagcaac ggaaaccgga agactatttg tccgaagatg tacgccgtcg
     2401 gctcccaagg caaggaccag gtcgatacga tcattcaata agatggttcc atgttggtgt
     2461 ttgataagag ggaagagctg ctgacaaagg gaagtcagat ctcgagtctt cagatctttt
     2521 tcgcggactt gcaccatccg gacaccggcg tctactgctt cggttatcac ggaggagagg
     2581 ggacgattga gagtttgatg ccggtcggtc agaagataaa gtttggggag aatgcgcgag
     2641 gacattccta attacgggtt acagcattcc ctcaataggg ctgctggctg tcgcatataa
     2701 cttcctggga attcttccag ccctgtaagc taatcgcccg gcatcaacgg cgtaccgcat
     2761 ggccgtggcc atcatcaacg gatctttggc tccggcaata gccgtgttca ttaatacggc
     2821 atcggctccg tattccatgg ccagggctgc gtcagaagcg gttccaactc cagcgtcaac
     2881 gatgaccggt acattgactg tttccatgat gattttgaga ttatacggat tgcgaatacc
     2941 gagacctgaa ccgatcggtg cggcgagtgg catcacggca ggacatccaa tatcggctaa
     3001 ctttttggcg acaatggggt cgtcattggt atacggtaat accacaaagc cttcggcgat
     3061 caaaattttt gcggcttcaa tgagtccggc cgtatcagga aatagcgtac gttcatctcc
     3121 aatcacctct aatttcacca aatctgaaac gcctgcagca cgggccagtc gtgaataacg
     3181 aacggcatcc tcaacggtat aacaccctgc cgtgttgggt aaaatggtat attttttcgg
     3241 atcaaggtaa tctagtaaat tttctgattt atgatcggtg atgttgacgc ggcgaaccgc
     3301 aaccgtcact acatcagctc cggaagcttc aatggctttt cgggtttcct caaagttttg
     3361 atattttccg gttccgaccc agagccgaga gcggaatgtt cgaccagcaa tgattaacgg
     3421 atcctgattc atctcttccc cttcaatcgc ctcatgtggg gcagccgcct ccaataaagc
     3481 ttaaaatttc aaccttgtct ccatcgtgaa gattccaggt gagaaaatct ccacggtcta
     3541 atatttttag gttgatttct actgcgactt gttcggaacg gaggtcaagc gttgagagaa
     3601 gttgtgcgac cgtcaacggt tcctcggcct cgcgttgttc tccattcacg actatgttca
     3661 tgcgtatagg taagaacgca atgtccagtg tattccggcc atgcagggat tcatcctacg
     3721 ggacttgaga aattcttgtc aatgaaatga acgagagtga agaaggtagg aagtggttcg
     3781 tgtgagccaa tgaaggctta tctatgtgaa ctggttttat cgatcacatc tgacataaaa
     3841 tgagaccgat attcattgga tagattaatg aagagatagc gtggtttgac tgagtcgatc
     3901 gtctatggag aaaaacatca accaggacct catggctttg ttcttgtcga tggcaggtct
     3961 gttggcgtcg agaggtgaaa atccctacaa ggtcaaagcc tatcgccggg ctgctgattc
     4021 gctgaagagc ctccaagagg atgtgacgga ggtggcgaat cgtggcgaac tccaaaccat
     4081 tcctggtatt ggcaaggaat tatcaataaa aatccaggag tttctctcca ccgggcgtat
     4141 ccgtgcgtat gaagaattaa aaactcctct tcctgattca gccctggatt gggtcaatct
     4201 tccgggattt tctgaaccga ttgtccatga tctggttttt cgattgggca tcacaagctg
     4261 ggaagatctg gaagccttgg ctcaatcgca tttactgcaa accctgccag gtctagggac
     4321 tcgtggtgag gaaatcttga cggcgattcg aaagaaacga gctctctgtc aggagtccta
     4381 acccgggaag taagaagggg aagcttctcc catggttccc cacgcatgca attcggcttg
     4441 ccaaggaaca tgaaatggat atggaagcat atcaaacttt cacctcagaa ggatggttag
     4501 gtaatggcaa tttctgcatt tgttttgatt gacacgacag ggaaccacac caagagtgcg
     4561 tataaaacac ttacacgcat tcaaggtgtg aaatccgtct atccggtgac cggtccgttt
     4621 gatttaattg cccatgtgga agccgaaacg ttggaggagt tgaacgacct catcatggtt
     4681 cgtcttcgag gtattgatgg tgtcattaaa acaaatacgg caattgtcct cgacttctaa
     4741 tttcttcaac atcttttgac ggccgcatta tgcggccggt tttaactgtt tgaaaacttt
     4801 ccatgatttc agcatctcca cagccttcag taactgaagg tcatctggag gaatgggctg
     4861 agcctggtcc ttgtcctgtg gggccgaggg ggcttgaggt ttggtcggag agggtgcagg
     4921 ggaatccgcg gtgctctcgt cttttttccc ttcggaggac tcttgtttcg ccacctgagc
     4981 gggtttcgga tcaatcacaa tgtcgggttg taccccgata gaatgaatcg acttgccatt
     5041 tggcgtaaag tattttgccg tggtaagccg aaggccggat ccatcggaga gcgggagaat
     5101 ggtttgcacg gaacccttcc caaaagttgt ggtgccaatc acgacggctt ttccccaatc
     5161 ctgcatggct gccgccacaa tttctgatgc gctggcggaa ccgtggttga ccaacacaat
     5221 catgggatat tgataattct tactacttgc ccttgcacgg tattcatcct tcttgccatt
     5281 gcgtccttgt attgagacga cgaggcggcc ggattccagg aattgttcgg agacccccac
     5341 cgcagcggtc aatagcccac cgggattatt tcggagatcg agaatcaagc cctgaatgtt
     5401 tttggcaacg agtttttcca attccgtgga gagatcctcg acggtggctt cttgaaattg
     5461 gctcaatcgg acatatccaa ggttgccttc caacagcttg gagcgaatgc tttgaatttt
     5521 aatgatgtcg cgtttgaggt taaagatcag tggtttttcc agtccctcac gttcaatggt
     5581 taattccact gatgtgcctc gtgcgcctcg catatgttga acggcttcca tgagtgtaag
     5641 atcttttgtc ggcttgtcat caactttgat gatggtgtcg ccgggttgga tgccggcttc
     5701 aaaggccggc gtatcttcaa tgggggagat cacggtcagg cgattgtttt ttacaccgat
     5761 ttggatgcca agtcctccaa actctccttt cgtttcaacc tgcatctctt tgaacatatc
     5821 cggagtcata aaagatgaat gtgggtccag tccagccagc atgcctcgga gggcaccctg
     5881 gacaaggtct ttggtttgag tttcttccac ataatgcttt ttgacttgcg aaagcacttc
     5941 agcgaagacc tgtaattcct cgtagggttc gctggcatat cccgcccggt ctcctccttt
     6001 tccgatgaca atccccacca aaaggatggc ggttaaggtt aaaccagaga tcactaccgt
     6061 cgacaatcgt tcatgtgaca tgagacgttc cttcttccag ttgttcaatt aaggctgttt
     6121 taccagccat ttcaggggat cgataggggt ggtgccttta cgtaattcaa aatacagggt
     6181 gtcttcctgg gtgacgcctg tttcgcctgt ttcaccaatg acctgcccgt ttttaacgga
     6241 gtggccttca gccactaaaa gttttgaggc atgggcgtag agagaataaa agccattggt
     6301 atggtcgaca atgataacca gaccatatcc cttgagccag tcggcataga cgacgtttcc
     6361 tgatgacact gcgcgaatcg gacttccttc ctgcacgctg atttctatgc cttttttcgt
     6421 cacaaatgta tcaaatgtgg gatgtttttg ccgaccgaaa tttgaaacca ctttaccttc
     6481 aaccggccaa atgaaagacc caagtgacgg gggagctcca gaaggtttgc gcatctggga
     6541 ttgagccagt tgaaaccgtt ggtccaattc tttgaggaga ctatccacct gttcagctga
     6601 gcgctgaaga tcttcaacca tccgttgatg tgcgtccttt tctttggaga gattaaccaa
     6661 gacggagcgt ttttcacgtt tgaggcctga aacttttttg atggtctgtt gagtttctcc
     6721 ctgaaagcga aggagatcct ccctggctgc ggtctgtttc tccttgagct gttggagatt
     6781 agagaggtct tcttgaaatt gtcggacaag ttgatgctcc cgttgggcca cccaggaaag
     6841 ataattcatc cggttcgagg cctcggtaaa ggattcagct gcgaagagcg tttttaaata
     6901 tccggtgcgt ccttccacat ataataacct gagtctggcg gcaatggagg attgtcgagt
     6961 gcgaacgtct tgctccactt cggtaatctt cgtgctcagg ttcgccaact cctcgtctgt
     7021 cgctttaatt tggtcattga tgcgattgtg ttctttcttt tgttcatgga gttggcgatc
     7081 caatttgtca atggttttta agaccgatcc atgtttcttc tcgatccgtt ttgtttcttt
     7141 tttagtttcg gtaatttcct gattgagctt ctccagccgg aggcgttcct gactgatttc
     7201 atttttgatg gttgaggggt cagcacccca accagcttgg acgaacagaa ggagtagtcc
     7261 gcttgtgagt cctgtgtgga gaaatagttt aacccgcctc ataaacgaaa ccgcaaggat
     7321 ccatagatgg tggtaagtgt tcccaggcac cccaatagga tgcctcctgc tatcaaaatc
     7381 agtgaaaggg gaagagggaa aaactccaat gcagaagaaa atcccccaaa gacattcaag
     7441 ccttgggtgt tttgcgtgaa aagttcgaac cctccacgta aaaaaccaag ggccataccg
     7501 cctcccgcgg cccctaatat agctccttcg atgagatacg gaatgctaat aaagagactg
     7561 gttgccccga ttaaccgtaa aatttcaatt tcttgctgtc tggtatggaa tgccaattgc
     7621 acggtaattg caatgatggt gacggacgct aagcccagga tactcccaac caataacgcg
     7681 ccgaactcca tataggcgat gatgagggtt aggcgctcga tccaatcacg attatatcga
     7741 acgcgatcaa tatcgggatg atgttgcagc cgattgacca ggttgccaat catatctgtg
     7801 gtaggggact ttgcggccat ggtcaatatc aatgatgcgg gaaacgggct ctcgccaagg
     7861 ccttccagta ggtagacttc ctcgggaaat tgttctttga attcggtcaa cgcgtcttgc
     7921 tttgaaatat aggtgaggga gtcaacggca ggatccgttt tgagtccctg ttgtaaatcg
     7981 tgtagcttgc cggattcaat atcattcttg ggatagacga tgatttgaat ggtgtgttga
     8041 acctgtccca atagattatg cacgttatga tagagaagga gaaagatgcc aaagctggtc
     8101 agggtaaacg ctgtggtcag aacaccaatc agcgtactgg tccgatgcgt gcggaggctg
     8161 gtataggctt cacggataaa ataccagagc cgtttcacgt atagactcct tgatcggata
     8221 cgagatgtcc gcaatccagt cggagaattc tcccatttaa ttgttctaca acttgtcgat
     8281 tgtgcgtggc catcatgacg gtggtccccc gggtattgat ggctttaaat aatgctgcaa
     8341 tttcggtggt gagttgggga tctaaatttc ccgttggttc gtctgccaaa agaatgatgg
     8401 gactgtttac gatcgcgcgg gcgatacaca cacgttgctg ctctccggcg gagagcacgg
     8461 aggcaatgga gttgagttta tgatccatgc caaccgcacg aagggcttcc aggacctttc
     8521 ttcgcgattc gaatgatgac aagccacgaa taatgagggg gagcgccaca ttttcggaga
     8581 ccgtccgctg tggaagtagt ttgaaatcct gaagaacata ccccatggtt cgtcgatgat
     8641 aggggatatc ggatcctccg agcctggcga tgttgcgatt ttggacgagg atttgtccct
     8701 catcagggtg ttctgctcca aaaataagct tgagtaaggt ggttttcccg gctccgcttg
     8761 gccctgtgag taacacacat tcgccttttt cgattcgcaa ggaaatatct gagagcgcaa
     8821 ggcagccatc gtagtatttt gtgacgtgga ataattgaat catgctatct cattggcctg
     8881 gcctataaat ggcttattat ctggatacaa ccggaccctg gttctttgtg aaagtaacaa
     8941 tgaagagctg ttcaccttca ggaattggac ttcgggcgga ggccaatccc tttaatttac
     9001 cattgccagt cggagctgga aacctcaaaa gcctgttcca gg
//
LOCUS       DAORAQ010000106         9028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1221_length_9028_cov_60.041123, whole genome shotgun sequence.
ACCESSION   DAORAQ010000106 DAORAQ010000000
VERSION     DAORAQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_9028_cov_60.041123"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1360
                     /locus_tag="PKM72_09710"
     CDS             260..1360
                     /locus_tag="PKM72_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP61105.1"
                     /translation="MNESVVLDKVIKTHGLVRAVDHVSLAIQNGEFFSILGPSGSGKT
                     TILRLIAGLDRPDQGDIIIQQRVVTLDPPHKRPVNMVFQHYALFPHLSVFENVAFGLK
                     MKGDGRANIQKAVGHMLALVRLQGKEKRLPGQLSGGEQQRVALARALVNRPVVLLLDE
                     PLAALDQQLRQEMQGELKRLQREVKATFICVTHQQDEALMLSDRIAVMHGGKLLQVGT
                     PQEIYDHPVSSVVAQFIGLSNVLSGIIASCDGGLCRVESNGLSPVMVSCSPHDFPQGA
                     VTVIVRPERVHVSSNMSANRYDNGLQAMMHHVAFSGNEVVYHVRLQDGAIWMARVPIA
                     EAQACPLTVGQQVYVQWWAHDGLVLPSQSPPC"
     gene            1309..2217
                     /locus_tag="PKM72_09715"
     CDS             1309..2217
                     /locus_tag="PKM72_09715"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP61106.1"
                     /translation="MVGTRWVGFALPISSMLTNRFATSSAFPVRLSLVSAGLFLGLFF
                     FLPLVLVFGVSLASRGLYGGISWIATWENYQRVFDPLVGVIFFRSLLLAGLTTLVCAV
                     VAFPLAYCLARASRPWQLILLTLVMIPFWTNFLVRTYAWVLIFRADGILNSLLIRVGF
                     LEEPLSLLFTDVSVFVGLVYGYLPFMVLPLVAAIERISPQLEDAAFDLYASMGAMFRH
                     VLLPLSAPGFLAGGVLVFIPSLGAFITPYLLGGGQNMMVGTFIQQEFLVARDWPFGSA
                     ISFGLMGSVLLIWFLLARTSVGKDPS"
     gene            2214..3014
                     /locus_tag="PKM72_09720"
     CDS             2214..3014
                     /locus_tag="PKM72_09720"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP61107.1"
                     /translation="MKRMPSSFILMSVLVMLFLYLPVVILIGLSFNASTMGVAWKGFT
                     FQWYEKLALDRAILEATVNSVVIAIISTGLALILGVGTAIGLETRQGGQRAWVNMILL
                     LPLVIPEILLGVALLMVFVLCQVRLGFGTIIIGHMVFNLPLTIVIVRARLRKLDPAWE
                     DAARDLGATSWDVLTRITLPLLRPAIWGAGLLGFTVSLDDFVVTFFVAGPGSTTLPLK
                     VFSMIRTGMTPEVNALSAVMVVVSMLCVGLSWFLQERSAPASSESSYL"
     gene            3028..4086
                     /locus_tag="PKM72_09725"
     CDS             3028..4086
                     /locus_tag="PKM72_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNP61108.1"
                     /translation="MNMLCQWAGILILLVAAGCSESSTSSESPSSQRPTLYYFTWSDY
                     VDAGVVAKFEATRGVRVVVDTFSSNEELLAKVQTGMTGYDVVVPSDFMAGIMGRLGLL
                     AELDLEKIPHVQDLELHLQQLSFDPTNRFAIPYLWGTVGMGYNSEVVTEPPTSWEALW
                     DPKFSGRISMLNDEREVFGLALQSLGYSLNSVDPQAIQLAKHKLMKQKPLIKAYTSEQ
                     FDQLLVAGEVVLAHAWGGPIARAMREHPSIRYALPQEGGMMWTDCLAVLASSSQQALA
                     MDFINFLLDEEVALATSQRLLFASANRHVRNRLPEEIRTNTAVYPPSEMLARMEWLED
                     VQEAIRYYDRAWTELKVH"
     gene            4171..4998
                     /locus_tag="PKM72_09730"
     CDS             4171..4998
                     /locus_tag="PKM72_09730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP61109.1"
                     /translation="MMQRMIISSQTICWVVVAFFLAGAPLWAEEPGTSRFPLYLTQMP
                     YEPPPPSESRGVEATPPPESLTTEDEERLEALIPLLEGKQEFWAIGEFVYFGKHSIPY
                     LVKALKSPVSRVRFNAVETLSMINDPSAVPGLLEVAINSEEDSRIRSHALRVATRLDP
                     NQVVPAIEVMVKDPNSTIRNTAVFESRRVHRKEVLPFIISAISDPEQYVSITARDSFW
                     ILTRFSGSIHDWEASTPEDRKEWMKEWWSWLEENQERFGETPSSTKPPRSPADLNVS"
     gene            5109..5639
                     /gene="def"
                     /locus_tag="PKM72_09735"
     CDS             5109..5639
                     /gene="def"
                     /locus_tag="PKM72_09735"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNP61110.1"
                     /translation="MAVLPIAKIGNPVLRQQAKPVDPASIGSRRIQEFLDDMFETMVH
                     YEGIGLAAPQVAHSEQIVVMECEGKDGIPKTVLINPKIVFYSPSQTEMWEGCLSIDLM
                     RGKVVRPSMIRVQAYDRQGVLQDFEANGLYAVCIQHEMDHLIGKLFIDRMADMSTLTQ
                     LEEFDAYWREESAPVI"
     gene            5766..7490
                     /locus_tag="PKM72_09740"
     CDS             5766..7490
                     /locus_tag="PKM72_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP61111.1"
                     /translation="MKSLLRVLSYLAPYRGLVCVTFFFAGVTTALELLPPWLIKVVID
                     DVIPAKNTDMLTWVLIGLLLAYGLKNLCNSLRIRFNNTLEQRVVHRLRQQVFAALQRL
                     SLTYYENRSTGEIMSRVVNDTEHVQRIFIDGLEGMLTATLTLVGITTILFMLNWKMAL
                     LVLLPIPILIIGGVAFTRRVHRYYHDIRQQAAELNGYLQDSLSGIRETMGFNRQPYEQ
                     TRFQDMSHHYSQANLKAMYLWSIYSPGMIFIGSLGTILIVWYGAGEVIAGHLSTGELV
                     MFLSYLVLFYTPVNQIHSVNHLLQHALAASERVFDILDAEPAVPDHGHLRPAQPRLSG
                     KVEWKDMAFSYRPGKPVYQHLSLVVEPGEHVALVGPSGVGKSTLMKLLFRYYDVGEGA
                     IELDGYDIRHLPLFYLREQIGFVQQEPFLFNGTVRSNLAYGDLAASQDQIEAVARAAQ
                     AHEFIQALPDGYDTWIGERGVKLSVGQKQRIAIARVLLKDPPIVVMDEATSNIDTETE
                     VEIRIAMDELMRGRTTFIVAHRLSTLQAVDRIVVFEYGQIVEEGDHATLLARGGLYAG
                     LYEAQFHV"
     gene            7594..7785
                     /locus_tag="PKM72_09745"
     CDS             7594..7785
                     /locus_tag="PKM72_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNP61112.1"
                     /translation="MTVKCPICGNVEQWEGNVFRPFCSKRCQLLDLDGWLSERYRIPD
                     AEEDGLDEAVRESGPTSAD"
     gene            7980..8519
                     /locus_tag="PKM72_09750"
     CDS             7980..8519
                     /locus_tag="PKM72_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61113.1"
                     /translation="MPKMITAKRHEDETDKAQEFVRTNILLPASLLGLISIVVGVIAL
                     GFQFWAGTYGWETFTYSSALIIAGVLVGIVQTKYQQYLLREFPGHFANRMRAYSQRSV
                     RKAKKAITEHDIEHRGRGFVPLWYLLGIVMFFALSGLAVATGFMEPVAAFALPWAGYF
                     WAKLFFWKGVVLLPDRKKK"
     gene            complement(8561..>9028)
                     /locus_tag="PKM72_09755"
     CDS             complement(8561..>9028)
                     /locus_tag="PKM72_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61114.1"
                     /translation="ELGANVTVDRATFGSTVIKRGTKIDNQVQIAHNVVIGEDCILVA
                     QVGIAGSTVLGRHVMVGGQAGLVDHVTIGDQVKIAAGSGVTNDVKSGQIVGGRPAVEH
                     GIWRRSQVIQYQLPELRKELRALQKQVKHLESLLPAQPAPKTERFKKPSPSKP"
BASE COUNT         1914 a         2164 c         2556 g         2394 t
ORIGIN      
        1 gcattaaccc aggcactccg ggatctcgcc ccgacggttc agaggcggac ttgcagtttt
       61 tgatgcctat gggtgcctgc aaggcctgaa ccggttctcc gaccattcgc catagccttc
      121 ttgtttgcac ccctcgcctt cgtcggtttg cctttggcat actgatgccg gtatcccttc
      181 attgacttcc attttcctcc cttcctagaa tcgtcaccct ggcggcttat ccaattctcc
      241 cggaagggat ctctggctcg tgaatgaaag cgtcgtcctc gataaggtta ttaaaaccca
      301 tggtctcgtg agggcggtgg atcatgtgtc cttggcgatt cagaacgggg aatttttttc
      361 catattggga ccgagtgggt cggggaaaac cacaatcctt cggttaattg ctggattaga
      421 tcggccggat caaggggaca ttataattca gcaacgcgtt gtcaccttgg atccccctca
      481 taagcgaccc gtgaatatgg tgtttcagca ttatgcgctc ttcccgcatc tgtccgtttt
      541 tgaaaatgtg gcctttggat taaaaatgaa aggagatggc cgggccaata ttcaaaaggc
      601 ggtgggtcac atgttggctc tggttcgttt gcaaggaaag gagaagcgac tccctgggca
      661 attgtccgga ggggagcagc aacgggtggc gcttgcacga gctctagtga atcgtccggt
      721 cgtcctgctg ttggatgagc ccttggctgc tttggatcag cagctacgcc aggagatgca
      781 gggagaactg aagcgattgc agcgtgaggt gaaggcgaca tttatttgcg tgactcatca
      841 gcaggatgaa gccctcatgc tctcggatcg aattgcggtc atgcatgggg gaaagttgtt
      901 gcaggtggga acgcctcagg agatctatga tcaccccgta tcttcggttg tggcccagtt
      961 tatcgggctt tccaatgtgt tgtcgggcat cattgccagt tgtgacggtg ggttgtgtcg
     1021 ggttgaatcg aatggcttgt cacccgtcat ggtctcgtgt tccccacatg attttccgca
     1081 gggggccgtc acggtcatag ttcgccccga acgcgtgcat gtgtcctcta acatgtctgc
     1141 caatcgatat gacaatggtc ttcaggcgat gatgcaccac gtggcctttt ccgggaatga
     1201 agtggtgtac catgttcggc ttcaggacgg ggcgatctgg atggctcgcg tgccaattgc
     1261 cgaagcacag gcctgtccct tgacagttgg tcaacaggtg tacgtgcaat ggtgggcaca
     1321 cgatgggttg gttttgccct cccaatctcc tccatgctga ccaatcgttt tgccacatct
     1381 tccgcatttc ctgtcaggct gtccctcgta tcggcaggcc tcttcttggg cctgttcttt
     1441 tttctcccgc tggtattggt ctttggagtg agtctggcaa gccggggatt gtatggggga
     1501 atttcctgga tcgcgacctg ggaaaattat caacgtgtct tcgatccgct cgtgggagtc
     1561 atattttttc ggtcgcttct cctggctgga ttaacgactc tagtttgtgc agtggtggcc
     1621 tttcccttag cctattgtct ggccagggct tcccggccct ggcaacttat attgttgacc
     1681 cttgtgatga ttccgttttg gacgaatttt ttggttcgca cctatgcgtg ggtgttgatt
     1741 tttcgggcgg acggtattct gaatagtctg ttgatcagag tggggtttct ggaggagccg
     1801 ctttccttat tgttcacgga tgtgtcggta tttgtgggac tggtctatgg ctatctgccc
     1861 tttatggtgc ttcctctggt ggcggccata gaacgcatct ctccccaatt agaagacgcg
     1921 gcgtttgatt tatatgcgtc tatgggcgcg atgtttcgac atgtgctgct cccgctcagt
     1981 gccccaggtt ttctggccgg gggagtgttg gtctttattc cttccctcgg tgcctttatt
     2041 acgccgtatt tattgggtgg aggacaaaac atgatggtgg ggaccttcat ccagcaagaa
     2101 tttctggtcg cccgggactg gccgtttgga tcagccatct cattcgggct gatgggaagc
     2161 gttcttctta tttggttttt gctagctcgg acgtcagtgg gaaaggatcc gtcatgaagc
     2221 ggatgccgag cagtttcatc ctcatgagtg tgctggtcat gctcttttta tatctgccgg
     2281 tggtcatcct gatcggccta tcatttaatg cctccacaat gggagtggcg tggaaggggt
     2341 tcacgtttca gtggtacgaa aagctggccc tggaccgggc gattctcgaa gcgacggtca
     2401 atagtgtggt gattgcaata atatcaaccg gattggccct tattcttgga gtggggaccg
     2461 ccatcggtct ggaaacccgt caaggggggc agagggcgtg ggtcaacatg atcctgttgc
     2521 tgccactggt catcccggaa atattattgg gtgtggcctt attgatggtg tttgtcttat
     2581 gtcaggtccg tctgggattt gggaccatca ttatcggaca tatggtcttc aatcttccgt
     2641 tgaccatcgt cattgtcagg gcccgtctcc gtaagctgga tcctgcctgg gaagacgcgg
     2701 cgcgtgattt gggcgccact tcatgggacg tgctcacccg catcacactt cccttgttac
     2761 gtccggcaat ctggggggca ggactgttag ggtttaccgt atcgttggat gactttgtcg
     2821 tgacattttt tgtcgccggc cccggttcaa ccaccctccc actcaaagtg ttttcgatga
     2881 tcagaacagg gatgactccc gaagtgaatg ccttatcggc ggtaatggtc gtggtctcca
     2941 tgctgtgcgt ggggttgtct tggttcctcc aggagcggtc ggcaccggct tcgtctgaat
     3001 cttcgtattt atgaagggcg gcaactcatg aacatgttgt gtcagtgggc ggggatcttg
     3061 attctcctgg tggcggcggg atgttcggaa tcttccacat cctcggaatc accatcctct
     3121 caacgcccta ctctttatta ttttacgtgg tctgactatg tggatgcggg ggtggtggcc
     3181 aaattcgaag cgacccgggg ggtgcgggtg gtcgtggaca cttttagtag caatgaagag
     3241 ttattagcca aagtgcagac cggcatgacc gggtatgatg tggtggtgcc ttctgatttt
     3301 atggcaggca ttatgggccg attggggttg ctggcggagt tggatctgga aaaaattcct
     3361 catgtgcaag atctggagct ccacctccag cagctctcgt ttgatcccac caaccggttt
     3421 gccattccct atttatgggg cacggttgga atggggtata attctgaggt ggtgacggaa
     3481 ccgccaacca gttgggaagc cttatgggat ccgaaatttt ccggtcgtat cagtatgctg
     3541 aacgatgaac gggaagtctt cgggttggca ctccagtcct taggctactc gctgaatagt
     3601 gtcgatcccc aggccattca actagccaag cataagttga tgaagcaaaa gcctttgatc
     3661 aaagcgtata cgagtgaaca gtttgaccaa ctacttgtgg ccggggaggt cgtattagcg
     3721 catgcctggg gagggccgat cgccagggcg atgcgtgagc acccgtcaat ccggtatgcg
     3781 ctcccccagg aaggtgggat gatgtggacg gattgtctgg ccgtcctggc cagttcctct
     3841 caacaagcgc tcgcgatgga ttttatcaat tttcttttag atgaagaggt ggcgcttgcc
     3901 acatcgcagc gactattatt tgcttcagcc aaccgacatg ttcggaatcg tctgccggaa
     3961 gagattcgca cgaacacggc ggtgtacccg ccaagtgaga tgcttgcccg gatggagtgg
     4021 ttggaagatg ttcaggaggc cattcgttat tatgatcgag catggacaga gctgaaagtg
     4081 cactaggctt ggcttgaccc ttttagccat ctgctactaa actgcatgat tgctaagttt
     4141 tatatacgag gattcacgaa agggaagtcc atgatgcagc gaatgataat ttcgtcacag
     4201 acaatatgtt gggtggtcgt ggcctttttc ctggccggcg ctccgctgtg ggcggaagaa
     4261 ccaggaacaa gtcgtttccc tctctacctg acgcaaatgc cctatgagcc tcctcctccg
     4321 tcagaatcaa gaggggtcga ggccacgcca ccccctgaaa gcttaacgac cgaggatgag
     4381 gagcgtctgg aagcacttat tccgctgctt gagggaaaac aggaattttg ggccatcgga
     4441 gagtttgtct attttgggaa acactcgatc ccgtatttag taaaagccct caagtcgccg
     4501 gtgtccagag tccggttcaa tgcggtggaa acgctgtcga tgatcaatga tccctccgca
     4561 gtcccgggct tactggaggt cgcaatcaat tcggaggaag actcccggat ccggtcccat
     4621 gcattgcgtg tggcgacacg attggatccc aaccaggttg ttccggccat agaggtgatg
     4681 gtgaaggacc ccaattctac gattcggaat acggcggtgt ttgaatcacg gagggtccat
     4741 cggaaggaag tgctgccttt cattatcagt gccatttcag atccagagca atacgtgtca
     4801 atcacggcgc gggattcttt ctggattctc accagattca gcggttcaat ccatgattgg
     4861 gaagcctcca cccctgaaga caggaaggaa tggatgaagg aatggtggtc ctggttggaa
     4921 gagaatcagg aacgattcgg cgagaccccg tcatccacaa aacctcctcg atcaccggca
     4981 gacctgaatg tcagttgaca gcggttcctg aataaccaaa cgagaggcaa gggccgtccc
     5041 ggatggtggt cgaaagcgtg tgatttcaag acgaaagacg tagaagattc agaaaaggag
     5101 tcaggttgat ggcagtatta cctatagcaa aaatcggcaa cccggttctc cggcaacagg
     5161 caaagccggt cgatcccgca tcaatcggga gtcgacgaat ccaggagttc cttgatgata
     5221 tgtttgaaac gatggtgcat tatgagggaa ttggattagc cgccccgcag gtcgcacatt
     5281 cggaacagat tgtcgtgatg gaatgtgagg ggaaggatgg cattccgaag accgtcctca
     5341 ttaatcctaa aattgtgttt tatagcccct cccaaactga aatgtgggaa ggatgtctaa
     5401 gtattgatct catgcgaggt aaggtggtcc ggccgtccat gatccgcgtg caggcctatg
     5461 atcggcaggg agtactccag gattttgaag cgaacggact ctatgcggtg tgcatccagc
     5521 atgaaatgga ccacctcatc gggaaattgt tcattgatcg gatggccgac atgtcgaccc
     5581 tgacgcagtt ggaagagttt gatgcctatt ggcgcgaaga atccgccccg gtgatttaat
     5641 ccctgcttga aagagctgac cgtcgagcgt tttctgctca tccattccac gagtgtgttt
     5701 tcgtgattcc atgattccca tgagaatcac gccatctggc gacaaaaaag gtttcgaaac
     5761 ttcctgtgaa atcgctcctg agagtcctgt cctacctggc cccgtatcgc gggctcgtgt
     5821 gtgtcacctt cttctttgcc ggtgtaacga cggccctgga gctgctcccc ccctggctca
     5881 tcaaagtggt gattgatgac gtaatcccgg caaaaaatac ggacatgctg acctgggtgc
     5941 tcattggact actcctggcc tatggactga agaatctctg taattccctc cgcatccgct
     6001 ttaataacac gctagagcaa cgggtggtgc atcgactccg acaacaagtg tttgccgctc
     6061 tgcaacggtt atcgttgacg tattatgaaa atcgatcgac gggtgaaatc atgtcgcgtg
     6121 tcgtgaatga tacggaacac gtgcaacgaa ttttcattga tgggcttgag ggcatgttga
     6181 cggccacttt gaccctggtc ggcattacca ctattttatt tatgctgaac tggaaaatgg
     6241 cccttctcgt tctactgccg attccgatac tcatcatcgg aggcgtggcg tttacccgcc
     6301 gggtccaccg gtattatcac gatattcgtc agcaggccgc tgaactcaat ggctatctgc
     6361 aggattcctt gtcggggatc cgggaaacga tgggattcaa tcgacagccc tacgagcaga
     6421 ctcgttttca ggacatgagt catcactaca gccaagcaaa tctgaaagcg atgtatttat
     6481 ggtcgattta ttctcctgga atgattttca tcggaagcct gggaaccatt ttgattgtgt
     6541 ggtatggcgc aggtgaagtg atagccggtc atctttccac tggtgagttg gtcatgtttt
     6601 tatcatacct cgtcttgttt tataccccgg tcaatcaaat tcattccgtg aatcatttgt
     6661 tgcaacatgc cctggctgcc agtgaacggg tgtttgacat cttggatgcg gagcctgccg
     6721 ttcccgacca cgggcatctt cgtccggctc agccccggtt gtcggggaaa gtcgaatgga
     6781 aggatatggc cttttcttat cggcccggga aaccggtcta tcaacatctt tccctggtgg
     6841 tggaaccggg ggagcatgtg gcgttagtcg ggccaagcgg tgtgggaaaa agtacgctca
     6901 tgaaacttct cttccggtat tacgatgtgg gggagggtgc gattgagttg gatggatacg
     6961 atattcgtca tctcccgtta ttctatcttc gtgaacaaat cggctttgtc caacaggaac
     7021 cctttttgtt taatggcacg gtgcgaagca atctagcgta tggagacttg gcggcatcgc
     7081 aggatcaaat agaagcggtg gcgcgggctg cgcaagcgca tgaatttatt caagcgctac
     7141 ctgatggata tgacacctgg atcggggaac gcggagtgaa attgtcggtt gggcaaaaac
     7201 agcgtatcgc cattgcccgg gtattgctca aagatccacc cattgtggtc atggatgaag
     7261 ccacgtcgaa tattgacacg gaaacggaag tcgagattcg gattgccatg gatgaactca
     7321 tgcgtggacg gacgacgttt atcgtggccc atcggttgtc aactctgcaa gccgtggatc
     7381 gaattgtcgt gtttgaatat ggacagattg ttgaggaagg cgatcatgcg accctactgg
     7441 cgagaggggg cttgtatgcc gggttgtatg aagcccaatt tcatgtttaa catggtatcg
     7501 catacatcca gctgctatac gcgtaatcga gcgctggtgg gttggggtcg gaactaaaga
     7561 cccgagtgac ccgagtctat cgtgagaaac gaaatgactg tgaaatgtcc gatatgcggc
     7621 aatgtggaac aatgggaagg caatgtcttt cgaccctttt gttccaagcg atgccaattg
     7681 ttggatttgg atggatggtt gagtgaacgc tatcgtattc ccgatgcgga agaagatggg
     7741 ttggatgagg cggttcgtga gtccggaccc acatctgctg attaggagag ggttagactg
     7801 aggaggaatg cgttgccaat atggcttcct gctctcaagt tcaccgcttt tctgagtcag
     7861 aggccaacga tgggcaattc gccaaaatag ggtttcgctc agccacattg accttttcgc
     7921 cctcgctcca tccagtccga gatgctcctg gtaaatgaaa ttataatgga gacaaaccta
     7981 tgccaaaaat gatcacggcg aaacgtcatg aggacgaaac agataaggcc caggagtttg
     8041 tccggaccaa tatcctactt cctgccagtt tgttggggtt gatttcgatc gtcgtcggag
     8101 tcatcgcctt aggctttcaa ttctgggcag gaacgtatgg atgggaaacc tttacctaca
     8161 gttcggcgct catcatagcc ggagttcttg tgggaatcgt tcaaacgaaa taccaacagt
     8221 atctattgcg agagtttccg ggacattttg ctaatcgcat gagagcctat agccaacggt
     8281 cggtccggaa ggccaagaag gccatcaccg agcatgatat tgagcatcgc ggtcgcggat
     8341 tcgttccgct ctggtatctt ctggggatcg tgatgttttt tgcgctttcc ggattagccg
     8401 tggctaccgg ttttatggag ccggttgccg cgtttgcctt gccctgggca ggctatttct
     8461 gggcaaagtt atttttttgg aagggggtag tgttgctgcc ggaccgcaaa aagaaatgag
     8521 gccttttctg ctgttgagtc ctggatcggc tatggacgga tcagggcttg gagggtgaag
     8581 gcttcttgaa ccgttctgtc ttgggagccg gttgggccgg aagaagcgat tcaagatgtt
     8641 tgacctgttt ttgaagagcc cgaagttcct tgcgcaattc ggggagttgg tattgaatga
     8701 cctgtgacct tcgccagata ccatgttcga ccgccggtcg cccacctaca atctgaccgg
     8761 atttcacatc attggtgacc cccgagcccg ccgcaatttt cacctgatca ccgatcgtga
     8821 catgatccac caggccggcc tggcccccaa ccatgacgtg gcggcctaac accgtactgc
     8881 ctgcaatccc cacctgggcg acgagaatac agtcttcccc gattaccacg ttatgggcga
     8941 tctgcacctg gttatcgatc tttgtgccgc gcttaatgac ggtgctgcca aatgttgccc
     9001 gatccacggt cacattggcc ccgagttc
//
LOCUS       DAORAQ010000107         9016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1226_length_9016_cov_64.220846, whole genome shotgun sequence.
ACCESSION   DAORAQ010000107 DAORAQ010000000
VERSION     DAORAQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1226_length_9016_cov_64.220846"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1250
                     /gene="fbp"
                     /locus_tag="PKM72_09760"
     CDS             243..1250
                     /gene="fbp"
                     /locus_tag="PKM72_09760"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006778.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNP61115.1"
                     /translation="MTPRFSLSTHTFREQALIPGTSGEFSRIIMQIALGGKLIAQDLR
                     KAGLSQFLGSTGLINVQGEEVQKLDERANQIFLDVFEHMELVSTLVSEEMEKPYLIKE
                     ADGQGRYAVFLDPLDGSSNIDVNASLGSIFSIHRLSVEGFPTSEDALRKKGSDQVAAG
                     YILYGSSVLLVYTCGHGVHQFTLDQEAGEFFLSARNIQIPKRGKIYSVNEGNHQKWST
                     GTQQFLHYLQEVDVQTGRPYTSRYSGCLVADVHRVLCKGGLYMYPGEKKNPEGKLRLM
                     YEAAPLSFLVEQAGGLGSTGGGSVNQLIPKALHQRVPLFIGSQEDVTKAEAFLRSELP
                     I"
     gene            1267..2292
                     /locus_tag="PKM72_09765"
     CDS             1267..2292
                     /locus_tag="PKM72_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNP61116.1"
                     /translation="MVLRMIFLVFAVVLGMALAWGETEGQTVLLLLGAGIGAAVGVII
                     LAVEQRLKVMSFPFVLCGGGGLIVGFLVAGLIGSVTGFGARFPYSVFNILTSLVFFLG
                     IPYWGLIMGIRFATEGWDTPTISTGDRDSVRIKKLLDTSVIIDGRIADLCETGFIEGT
                     LVVPHFILQELQHISDSSDGLKRARGRRGLDILNVLQKVSNVKVELVEDDFPHVKEVD
                     TKLIELAKQMDAKVLTNDFNLNKVAGIQGVRVLNINDLCNALKPVVLPGETIRVFVLK
                     EGKESGQGVAYLDDGTMVVVDHAKRWIGKNADVIVTSVLQTSAGRMIFTRLKEETEHE
                     ELSFSRV"
     gene            2285..2989
                     /gene="ispD"
                     /locus_tag="PKM72_09770"
     CDS             2285..2989
                     /gene="ispD"
                     /locus_tag="PKM72_09770"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNP61117.1"
                     /translation="MFNSVVAVVPAAGLGIRMGGNTPKQYLSLGGIPLLVYSLKVFQH
                     LECIREVILSVPASDLDYCRREIVQLFGLKKVTQVVAGGLRRQDSVRKGLEAISDQPD
                     GVLIHDGVRPFIDQPIVRNVIDRAGETGAAVVAMSIHDTVKRVDHSGIIQETLKREEL
                     WQIQTPQVFRYDWLVEAHKQAQLHQWDVTDDAALIERMGYPVSVVEGSCFNIKVTKPD
                     DLVLGEAILKTMGSRG"
     gene            3048..3530
                     /gene="ispF"
                     /locus_tag="PKM72_09775"
     CDS             3048..3530
                     /gene="ispF"
                     /locus_tag="PKM72_09775"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNP61118.1"
                     /translation="MRVGQGYDIHPLQEGRPLILGGVAIPHTQGLDGHSDADALTHAV
                     CDAILGAMGEGDLGTRYPSSNPEFKNLSSLVMLRSVAQTLREKGFYLVNLDTVILAQA
                     PKLAPYMVSMQESLADVLQVSPSQVNVKVKSGEGIGMIGRAEGIATLAICLIESIAPL
                     "
     gene            3584..4222
                     /gene="cysE"
                     /locus_tag="PKM72_09780"
     CDS             3584..4222
                     /gene="cysE"
                     /locus_tag="PKM72_09780"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNP61119.1"
                     /translation="MVKRITEDLHAVFERDPAATSRWEVLLAYSGFHALLAYRVAHWL
                     WKKNVPIVPRLISQLARWLTGIEIHPGAQIGRGFFIDHGMGVVIGETAVIGESVTLFQ
                     GVTLGGTGKERGKRHPTLGNHVVVGAGAKVLGNITIGDFVKIGANSVVLRSVPSNSTV
                     IGIPGRIIKTIGDRVPEATMDHANISDPIAERFDAMEQELIALRKQVEQSEK"
     gene            4336..5277
                     /locus_tag="PKM72_09785"
     CDS             4336..5277
                     /locus_tag="PKM72_09785"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNP61120.1"
                     /translation="MKEPNVITPRVKEILRNYEGNVPGVLANIARLLMTGRLAGTGRL
                     VILPVDQGFEHGPARSFAVNPAGYDPHYHFQLGIDAGCNAYAAPLGFLEAGAAEYAGQ
                     IPLILKLNNNDSLFESKDPNSAITGSVHDALRLGCCAVGYTIYPGSAHSQEMYNHLRE
                     IAQEAKSHGLAVVVWSYPRGSGLSKEGETGIDIVAYAAQIAAQLGAHIVKVKVPSNHI
                     EQAAAKKVYESEKIPIGTPAERIRHVVQSTFQGRRIVIFSGGAKGEDQKIFDEARGIR
                     DGGGFGSIIGRNSFQRPRPQALEFLSTVMKIYGEELI"
     gene            complement(5311..6585)
                     /locus_tag="PKM72_09790"
     CDS             complement(5311..6585)
                     /locus_tag="PKM72_09790"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNP61121.1"
                     /translation="MTNDLAPEFNHPTFRLAVAQFDEAAHHMNLDSGLLERLKAPQRS
                     LCVSIPVRMDNGQVQVFRGYRVHHDVARGPTKGGIRYHPDVSLGEVAALAMWMTWKTA
                     LAGLPFGGAKGGVAVNPSMLSAAELEGVTRRYIAEIFPLLGPDKDIPAPDIGTNQQVM
                     GWVMDTYSQQVGYTVRGMVTGKPISIGGTLGREEATGRGVVDVTLEVLRNFGLSTSDT
                     TVVIQGFGNVGSHTARILHQEGVKILAISDQLGGLYNKKGLDIPGILSHLATEKASVP
                     SLTQFGEPISNEELLMLPCHVLIPAAVSEQITVNNASGIQCQYLIEAANGPTTLEADA
                     ILRERGIFVVPDILANAGGVIVSYFEWVQDAQRFSWQESDIHSRLKNIITVAFHRILK
                     HAEEKKLTMRTAALIAGIQEVAQAHQCRGLYP"
     gene            complement(6851..7585)
                     /gene="truA"
                     /locus_tag="PKM72_09795"
     CDS             complement(6851..7585)
                     /gene="truA"
                     /locus_tag="PKM72_09795"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNP61122.1"
                     /translation="MSTIKLTIEYDGTAYAGWQRQPNQPTIQAALETALTRITQQHIS
                     IIAAGRTDAGVHARGQVASFQSDKSIPADKWKLALNSALPPDISVVTSERVPESFHAR
                     YSAKEKLYEYRISNHPARPAIDRHRVWHLPYELDTQAMRQAMSGFIGRHDFTSFQGPR
                     ASTSDPICVVSEFSLTSDLMTLTIQIQANRFLKQMVRTIIGTLTEVGRYKRSPDSITD
                     ILQAKDRRAAGVTAPPQGLYLIHVLY"
     gene            complement(7675..8919)
                     /locus_tag="PKM72_09800"
     CDS             complement(7675..8919)
                     /locus_tag="PKM72_09800"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNP61123.1"
                     /translation="MPSTRMIPLYSCIGMLVLSTSFPSSGGATDTRPFSGDEIPPLIH
                     LIAQSPPSTSIRNIRAHRHKQYTRVVLDLNRPVHPAPQEHQTATTFQLELPNTQLPQS
                     TVAKSTADSFPLQLDLSRNSSGSILLTIPIDGWKHYKWYVLKNPARVVLDLYPIAEGA
                     AGIKAPPSNTPASPVVAPPTPPPPVIKKDLVIVIDPGHGGKDPGALGRKGTREKDVVL
                     TIANHLRDLLAKEKSTKVFMTRETDVFIELEDRAKFANTHKADLFVSIHINSHPQASV
                     KGMELYHFGEASDPRALAVAARENGTPLEKNAAPWQFILADKLNDKKIDDSRELAWTT
                     KNSLVKFLDAFYKIKDHGIKTAPFFVLRMTTMPAILAEIAFISNPTEEKLLQSSTYQK
                     RVAEGIFKGLQSYITPLQTASR"
BASE COUNT         2223 a         1925 c         2459 g         2409 t
ORIGIN      
        1 ctttttcgat ttcagtttgt tcctgagccc attctcaatc attcctggtg atggcatagt
       61 cgatcggatc aaggatcggt gatgtccatc actcatgaag gctcgtcatt caacaacgca
      121 gccgagtccg tcattggagt tttttcaata tcgaagccat atttctgtca aacgtgcgtt
      181 gctcctttat gcggaaaagc atataatgaa aatcctctag aattccattt ggatcgtgat
      241 tgatgactcc gcgtttttcc ttatcgacac atacctttcg tgagcaagcg cttattcccg
      301 gaacctccgg agaattttct cgcatcatca tgcaaattgc cctgggtggg aagctgattg
      361 cccaggatct gagaaaagcc ggattgagcc aatttctggg ttctacaggg ttgattaatg
      421 ttcaaggcga agaggtccaa aagttggacg aacgagccaa tcagatattc ctggatgtgt
      481 ttgagcatat ggaactggtg agtacgctcg tttcagagga aatggaaaaa ccctacctca
      541 ttaaagaagc tgatggtcaa gggcgatatg cggtcttttt ggatcctttg gatggctcct
      601 ctaacattga tgtgaacgcg tccttagggt caattttttc tattcatcga ctttccgttg
      661 aagggttccc cacttctgaa gatgccctgc gaaaaaaggg gagcgatcaa gttgcggctg
      721 gatatattct ctatggatcg agtgtgctgt tagtctatac ctgcggccat ggggtgcacc
      781 aatttacgtt ggatcaggag gcaggtgagt tttttctctc agccaggaat attcaaatcc
      841 ccaaacgtgg gaaaatttat agtgtgaatg aaggcaatca ccaaaagtgg tctaccggca
      901 cacaacaatt tcttcactat ctacaagaag tcgatgtcca gacaggcaga ccctacacca
      961 gtcgttattc cgggtgtttg gtggctgatg tgcatcgtgt tctatgcaag ggtggcttat
     1021 atatgtatcc gggagaaaag aaaaaccccg aggggaaatt gaggctcatg tatgaagccg
     1081 cacccctatc atttttggtt gaacaggccg gtggcttggg gagtacggga ggcgggtctg
     1141 tcaatcaact cattcccaaa gcccttcacc agagagtccc tctatttatt ggcagtcagg
     1201 aggatgtgac caaagctgaa gcgttcctgc ggtctgagtt accgatctag ggaaggagaa
     1261 tctcttatgg tgttgcgaat gatatttctg gtttttgcgg tggtcctggg catggccttg
     1321 gcatgggggg aaactgaggg acagacagtc ttactgctcc tgggcgcggg aataggggcc
     1381 gcagtcggag taataattct ggctgtagaa cagagattga aggttatgtc tttcccgttt
     1441 gtcttgtgtg gagggggcgg attaatcgtt ggtttccttg tggcaggact gattgggtcg
     1501 gtgacgggat tcggcgcacg ctttccttat tccgtcttta atattctgac gagcttggtg
     1561 ttctttttgg gaattcccta ttggggattg atcatgggaa tccgatttgc cactgaagga
     1621 tgggacacac caactatttc aacgggtgat agggattccg ttcgcatcaa aaaacttctg
     1681 gataccagcg tcattattga cggccggatt gcagatctgt gcgaaacggg ctttatcgag
     1741 ggaacccttg ttgttccgca ttttattctt caggaattac agcatatttc cgattcctct
     1801 gacggattaa agcgggctcg tggaaggcga gggttggata ttttgaatgt tcttcaaaaa
     1861 gtgagcaatg tcaaggtgga actggtagag gatgattttc ctcatgtaaa agaggtggat
     1921 acgaaactca ttgaactggc aaagcagatg gatgcaaaag tgctcacaaa cgatttcaat
     1981 ctgaataagg tagctgggat tcaaggcgtg agagtgctca atattaatga cctctgtaat
     2041 gcgctgaagc cggtggtact cccaggtgaa acgattcgag tctttgtctt gaaagaggga
     2101 aaagagtcag ggcaaggtgt ggcctattta gatgatggca ccatggtggt ggttgaccac
     2161 gccaaacgat ggatcggcaa aaatgccgat gtgattgtca caagcgttct ccaaacaagt
     2221 gctggccgaa tgatctttac ccgtctgaaa gaagaaaccg aacacgagga gttaagcttt
     2281 tcgcgtgttt aattctgttg tggcggtcgt ccccgccgcg ggtcttggta tccgcatggg
     2341 gggaaacact cctaaacaat acctttctct tggaggaata cctctcctag tatattcact
     2401 aaaggttttt caacatctcg agtgtatccg cgaagtgatt ctttccgtgc cggcgtcgga
     2461 tctggattat tgccggcgtg aaatagtgca gctctttgga ttgaagaaag ttactcaggt
     2521 tgtggcggga ggactacggc gtcaggattc tgtcaggaag ggacttgagg ccatttccga
     2581 tcaacctgac ggagttctga tccatgatgg ggtccgtccc tttatcgacc agcccattgt
     2641 taggaatgtg atcgaccggg ccggggaaac aggggctgcc gtggtggcga tgtcaattca
     2701 tgataccgta aaacgggtcg atcattctgg gattattcaa gagacattga aaagggagga
     2761 actctggcag attcaaactc cccaggtgtt tcggtatgac tggctcgtag aggcacataa
     2821 acaggcgcag ctacatcaat gggacgtcac cgatgatgct gccctcatcg aacggatggg
     2881 ataccccgtt tccgttgtgg aaggaagttg ttttaatata aaagtgacca aaccggatga
     2941 tctggttctg ggggaagcta ttttgaaaac gatgggaagc cgagggtgaa acctccagct
     3001 agacttcatc aggtgtgttt tttttctctc ctcattccta attcactatg agagttggcc
     3061 aaggttatga cattcatccc ttgcaagagg gccgtccact gattctgggt ggcgtggcta
     3121 tcccacatac acaagggttg gatggccatt ccgatgctga tgctctgacc catgccgtct
     3181 gcgacgccat tctgggagct atgggagaag gagatcttgg aacacgctat ccaagcagta
     3241 atccggaatt caaaaatctc tccagcttgg tgatgctacg gagtgtcgct caaacccttc
     3301 gtgaaaaagg attttatttg gtgaatctgg atacggttat tctggcccag gctccgaaat
     3361 tagccccgta tatggtttcg atgcaagaga gtcttgcaga cgtgctgcag gtttctcctt
     3421 ctcaggtaaa tgttaaagtt aaaagtggag agggaattgg aatgattgga cgggcagaag
     3481 gtattgccac gttggcaatt tgtctcattg aaagtatcgc gccgttatga aatgatacca
     3541 cctccaactt cttggggtat atccatatgg tgggcctatg cggctggtta aacgaattac
     3601 tgaagatctt cacgctgttt ttgagcgcga tccggcggca accagtcgtt gggaggtgtt
     3661 gctggcctac tcgggttttc atgcgttatt ggcctaccgg gtggcgcatt ggttatggaa
     3721 gaaaaatgtt ccgattgtcc ctcggttgat ttcgcaactg gcccggtggc taaccggtat
     3781 agaaattcat ccaggagcgc agattggacg cggatttttt attgaccatg gaatgggggt
     3841 ggtgattggc gagactgccg tcattggtga gtctgtcacc ctctttcaag gcgtcacact
     3901 cggaggaacc ggcaaggaac ggggtaaacg ccatcctaca ttggggaatc atgtcgttgt
     3961 cggagccgga gcaaaagtgc tagggaatat tacgattggg gattttgtaa aaattggagc
     4021 caattcagtt gttcttcggt cagttccttc gaattcaaca gtcattggga tacctggccg
     4081 tattatcaaa accattggag accgggtgcc agaagcgacc atggatcatg ctaatatttc
     4141 agatccgatt gctgaacggt ttgatgcgat ggaacaagaa ctcattgctc taagaaaaca
     4201 ggtggaacaa tctgaaaaat gaccgtaacg gatgtgggag aattgttatt attgtcttag
     4261 gccgtgaggt cttctacact acaggcgagg gtctgaacaa agagaaagta ctatgttgaa
     4321 agggagggat tttttgtgaa ggaacccaac gtaatcacgc cacgagtcaa ggaaatctta
     4381 cgaaattatg aggggaatgt ccctggagtg cttgcaaata ttgcccgttt attgatgacg
     4441 ggacgattgg ccgggactgg tcgcttagtt attctgccgg tggatcaagg ctttgagcat
     4501 ggtccagcca gaagttttgc ggtcaatcct gcagggtatg atcctcacta ccatttccag
     4561 cttggcattg atgccggatg taatgcctac gcggctcctc tcggatttct agaggccgga
     4621 gctgcagaat atgccggtca aattcccctt atactgaaat tgaacaataa tgattcgctg
     4681 tttgagagca aagatcctaa ttccgccatt acgggcagcg tccatgatgc cctgcggtta
     4741 ggatgctgtg cggtgggata tacgatttat ccagggtctg cccatagcca agagatgtat
     4801 aaccatttgc gggaaattgc tcaagaggcc aaatcccatg gattggcggt agttgtatgg
     4861 tcatatcctc gaggttctgg actgagcaaa gaaggggaga cgggaattga tattgttgcc
     4921 tatgccgcgc aaattgccgc ccaactaggg gcccatatcg tcaaagtgaa ggtgccatcg
     4981 aatcatatag agcaagcggc tgctaaaaaa gtatacgaat ccgaaaagat tcccattggc
     5041 acgccagctg aacgtatccg ccatgtggtg cagagtacat ttcagggaag gcgtattgtc
     5101 attttttcgg gcggggccaa aggggaagat cagaaaattt ttgatgaagc cagaggaata
     5161 cgggatggtg gaggattcgg gtccattatt gggagaaact cctttcaacg gccgagacct
     5221 caggctcttg aatttctctc aaccgtcatg aagatctatg gtgaagaact catttagaga
     5281 tttccctcgc caggatttag aatgtataaa ctaggggtac aatcctcgac attggtgggc
     5341 ttgagcgact tcctgaattc ctgcgattaa tgcggcggtt ctcattgtca gttttttctc
     5401 ctcagcatgt ttgaggatac ggtgaaacgc tacggtaatg atgtttttga gccggctgtg
     5461 aatatctgat tcctgccacg aaaaacgttg ggcgtcctgg acccattcaa aatacgagac
     5521 gatcactccg cctgcattgg cgagaatatc cggaaccaca aaaattcctc gttccctcaa
     5581 aatggcatcg gcctccagag ttgttgggcc gttggctgcc tcgataaggt actggcattg
     5641 aatgccagaa gcgttgttaa ccgtgatttg ttcggacacg gcagctggaa tgagcacatg
     5701 gcacggtaac atgagtaact cttcgttact aatcggttca ccaaattgcg tgagagaagg
     5761 gaccgacgct ttctcggtgg ccagatgaga aagaattcct gggatatcca gacctttttt
     5821 gttgtacaac ccgcccaact gatcgctgat tgccaatatt tttacgcctt cctgatgtag
     5881 aattcgtgca gtatgggatc cgacgtttcc aaacccctga atgaccacag ttgtgtctga
     5941 agtagacaga ccaaaattac gtaggacttc caacgtgaca tccaccactc cacgccctgt
     6001 cgcctcctcc cggccaaggg tgcctccaat tgagattggt ttcccggtga ccattcccct
     6061 gacggtatat cctacctgct gactgtaggt atccatcacc caccccatga cttgctgatt
     6121 ggtcccaata tccggggcgg gtatatcttt gtccgggccc aataacggaa agatttctgc
     6181 gatgtaacgg cgggtcacac cttccaattc tgcggctgaa agcattgaag gattgaccgc
     6241 caccccgcct tttgctccac caaatggcaa cccggctagc gctgttttcc aggtcatcca
     6301 cattgccaag gctgcgactt cgccaaggct gacgtctgga tggtaacgaa tgccgccttt
     6361 ggtggggccg cgggctacat catgatgcac tcgatagcca cgaaagactt gaacctgtcc
     6421 gttatccatc cggacaggga tactgacaca gagagaccgc tgaggggctt tgagtcgctc
     6481 aagcagacct gagtccagat tcatatggtg ggctgcctca tcgaactggg ccactgccaa
     6541 gcgaaacgtt gggtggttga actctggagc aaggtcattg gtcatggggg ttatccagcg
     6601 agttgaaaat gttaaatctt actttttagt atatcaagaa tgcgatttta aataattcct
     6661 ttttacatcg gcatttgagt ggatgaaaag aaagcaagaa aaatgctgcg gtattcaaaa
     6721 atgtttgtca tgccctgccc tgagtattgc tgaaggggag gctgcagatt ttttgacggg
     6781 atgagcggaa gagtaggcga gaacgatcaa tgagtgggaa cgcaggtcgg gccatgtttc
     6841 aacactccaa ttaatacaac acatgaatga ggtagagccc ttggggaggg gcggttactc
     6901 ctgcagcgcg tcggtcctta gcctgaagaa tgtctgtaat cgagtccggt gatcttttgt
     6961 atcgtccgac ttcggtcagt gtgccaataa tcgtccgaac catttgcttc aggaatcgat
     7021 tagcctgaat ctgaatggtg agagtcataa gatccgaagt gagggagaat tcggacacga
     7081 cgcatatggg atccgatgta ctcgcacgtg ggccttggaa cgaggtgaag tcatgtcggc
     7141 caataaatcc agacatggcc tgtcgcatgg cctgggtatc caactcgtag ggtaagtgcc
     7201 aaactcgatg gcgatcaatg gccgggcggg caggatggtt ggatattcga tactcgtaca
     7261 gtttttcttt tgcgctatat cgagcatgaa acgattcagg aacacgttca ctcgtcacca
     7321 ccgaaatatc aggtggaaga gcgctgttga gcgccagttt ccatttatct gctggaatag
     7381 atttgtcaga ttgaaaactg gcgacttgac cacgggcatg gaccccggca tccgttcgtc
     7441 ccgcggcgat gatagaaatg tgttgttgtg taatgcgggt aagcgctgtt tctaaggcgg
     7501 cttggatcgt cggctgattg ggttggcgtt gccatcccgc gtaagccgtg ccgtcatatt
     7561 cgatggtcag tttaatggtg gacacgggtt actgtgttac caggggatga gcggggtaat
     7621 cttcataaac aggagatcgg agaatccgat ggaagaggcc gggggctgtc ctggctatcg
     7681 agaagcggtt tgtaacgggg taatatagga ttgaagtccc ttaaatattc cctcggccac
     7741 tcgtttttga taggttgaac tttgtaatag cttttcctcc gtaggattgg agataaaggc
     7801 aatctctgcc aatatggcag gcatggtggt cattcgcagc acaaaaaatg gagcggtttt
     7861 gatcccatga tctttgatct tataaaaggc atccaaaaat ttcaccaggg aatttttggt
     7921 tgtccatgct aactcgcggg aatcatcaat ttttttgtcg ttaagcttgt cggcaagaat
     7981 aaattgccag ggtgccgcat ttttttcaag cggtgtgcca ttttctctgg cagcgacggc
     8041 aagtgctcgt gggtcactgg cttcaccaaa gtgatagagt tccatccctt tcacggaagc
     8101 ttgaggatgg gagttaatgt ggatcgagac aaagagatcc gctttatgtg tatttgcgaa
     8161 tttggcccgg tcttccaatt cgatgaagac atccgtttct cgggtcatga agactttcgt
     8221 ggatttttct ttggcgagca ggtctctcaa atgatttgcg atagttaaga cgacatcctt
     8281 ttcccttgtg cctttgcgac ctaaggcccc tgggtctttt cctccgtgtc caggatcaat
     8341 cacgatcacc agatcttttt ttatgacagg tggtggcgga gtgggtggag ccaccactgg
     8401 agaagctggt gtgttacttg gtggcgcctt aatgccagcc gccccctcag ctataggata
     8461 caaatcaaga acgacgcgag caggattttt gagaacatac cacttatagt gtttccagcc
     8521 gtcaatgggt atggtcaaga ggatggaccc agaagaattt ctggaaagat ccaattgtaa
     8581 tggaaatgaa tctgccgttg atttggcgac agtagactga ggtaattgtg tgttgggaag
     8641 ttctaattga aatgttgtgg ccgtttgatg ctcttgagga gcaggatgga cggggcggtt
     8701 tagatctaac acaacacggg tatactgttt atggcgatga gcacgaatgt tgcggatgga
     8761 ggttgagggt ggtgattgtg caataagatg aatcagtggg gggatttcat ccccagagaa
     8821 aggtcgagtg tctgtcgctc ccccagacga gggaaaagac gttgacagga ccaacatacc
     8881 gatgcaggaa taaaggggta tcattcgagt ggaaggcata ggtattaaac cattcctctc
     8941 ttttttcgca gaagaaagcc ttcttgtcaa gggaagaagc gctttcgaac cgggtcctgt
     9001 tgcgggtaga ctcagt
//
LOCUS       DAORAQ010000108         8967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1236_length_8967_cov_69.203995, whole genome shotgun sequence.
ACCESSION   DAORAQ010000108 DAORAQ010000000
VERSION     DAORAQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_8967_cov_69.203995"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..1700)
                     /locus_tag="PKM72_09805"
     CDS             complement(333..1700)
                     /locus_tag="PKM72_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP61124.1"
                     /translation="MINDSPAILVVDDEPEMQQLLRDILEEEHYRVMVASDGHDALNR
                     METEKFPVVLTDLRMKGMDGLALLDHVLRKHPESNLIMMTAFGTVESAVDAMKLGAFD
                     YLTKPIKSNELSVTVAKAMREALLRQEVKQLRQQVSREFGFGQILGKSKPMKEIFDLI
                     RRVADSQTNILITGESGTGKELVAKAIHFNSQRKSAPFVPVNCAAIPELLLESELFGH
                     VRGAFTDAKSDKPGLFEEAHDGTLFLDEISEMPMMLQAKLLRAVQEREIRRVGATRSM
                     SINVRLVVATNVQLVEEVKAKRFREDLYYRLNVIELHLPPLRDRKEDLPILVQGLLQK
                     SVTAQQKHIESVEEPAMARLMDYQWPGNVRELENILERAATLTQGKKICLDDLPSNIR
                     NIQGDGQLIGDAAERLLPLEQLEKSYIRRVLEKMGGNKYQTAHVLGIDRKTLYRKLAE
                     MEEVV"
     gene            2045..2908
                     /locus_tag="PKM72_09810"
     CDS             2045..2908
                     /locus_tag="PKM72_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61125.1"
                     /translation="MKIVVPIDGSTCSTFAVEALAHFKPPEELTLVHALQLPDFNYPM
                     ITPDLRTEAQEEIKTQLRKEGEGILDEAQAHLPADFSHVQRLHQIGHPVDVIIETAQS
                     VQSNLILLGARGLGPVKKLILGSVSHRVLMHAPCPTLIVKTSMTRLQKILLPIKGQED
                     ADLALQFLALQPFRQPVEVEVFAVWPQPQLSWPTTVGQSDALEAQAIEEAQERMKTIT
                     DRLTRMNYASQAHVGMGDPAYATLEQAKASQSDLIMMGTHDRKGFSRFLMGSVSNAVL
                     HQTPCPVMIVR"
     gene            3147..3602
                     /locus_tag="PKM72_09815"
     CDS             3147..3602
                     /locus_tag="PKM72_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNP61126.1"
                     /translation="MSAITRWDPFREMDELQGRLSTLFGRAPVRKEGERQEALRVAEW
                     APLVDITEDQKEYVVKAELPEVKKDEIKIGVQNDVLVISGERKYEKEEKEKKYHRIER
                     AYGSFSRSFTIPEDADPEKVSAEFKEGILQVHLPKSDRAKPKSIAVKVS"
     gene            3722..4147
                     /locus_tag="PKM72_09820"
     CDS             3722..4147
                     /locus_tag="PKM72_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61127.1"
                     /translation="MHSLGEYLFRKFGILFLGFFLIGGIPPSSAQPLQEVKVTIEGYT
                     FQTTQMPLQLDTETIIYITNKDTVRHDFGSDMFLHTLTHVDSNGVITYGKGVEGVLLE
                     PGQAASIHLMLNHSGRFQFQCSIHKEMKGEILLLTVDAV"
     gene            4255..5217
                     /locus_tag="PKM72_09825"
     CDS             4255..5217
                     /locus_tag="PKM72_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61128.1"
                     /translation="MVKIVTHVLFPTDFSPSSAPAFRYAVEWAKVFEAQLTILHVHSL
                     QPGLDIDAGVAQQFLDEQRKVAREELETLLAEARQQVPKASMELMAGLPSECICEVAR
                     EKKCDLIMMGTHGWTGINRVLFGSVAERVIQRAPCPVLSIPHRESADISAMHPLQILP
                     RQVVLPLEFSDCSMEAYEYAIQIAKWFDVPLTLIHAIEPLSYSLDFTLTHPLQEKTNR
                     DKVEKRLADLTAVLTEQGLSARYELLDKPTVDGILETSAIQQADLIVMGSHGRKGLTR
                     MILGSTAYKVLEQSPYPVLTVKSPKFEGGHHPSLANGKTTSSPT"
     gene            5235..6602
                     /locus_tag="PKM72_09830"
     CDS             5235..6602
                     /locus_tag="PKM72_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61129.1"
                     /translation="MADHHSHPIAGSEEGLRSLLTRLEHQSLQPDFQIQRQLALSHAL
                     QPYFHPLMVPPLAPLPEEEDLAKWYLYADYLPTDGHASLIEQVRDLVTEHVPQEERVW
                     LDSLRHSYMDLLEVQEMASGKQGTQTRLQSLGDKQIFDVLPHATPVPYKIGQILLTRL
                     LRGLADIRLPGPPLVFSASMGKAVFKLTNERRQEIEIGTGNFALSEWAEFAKSYGYEM
                     MWSVAKVRRGAWTVVDSQMDYRNTQGETFLYAIALYEHQDFRTLADGLRGFDQFTPIQ
                     ENAGKPVSSASGSDTLTWVWLPPQNPSGDTGSPVARLTLTPTQLCVETDAPDRLNIVK
                     HQLAAVFGFSLHFLGETIDSPQHRTPEVDLLSDTYILPPTIVSQEEEQQILASLLETV
                     YLDWAETPSPILKGQSPRHFCSAQQDTKEVAAIIDQMEQHDPGRRRTGQPAYDYNILR
                     GHIGL"
     gene            6842..7963
                     /locus_tag="PKM72_09835"
     CDS             6842..7963
                     /locus_tag="PKM72_09835"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNP61130.1"
                     /translation="MATESFEPSPPALLLRKDFSQLIQVLQNLGYRILGPVLSNGAIQ
                     WDEITREEDLPIGWKDQQHPGNYRLEPDPSARYFNIVHGPQSLKPLVFSPRETLLVLD
                     RNEKTFSAKEIIPDIPRTAVLGVRACDLAGLGIQDQIFLKGPYPDPYYQQRRNNLFLI
                     AVNCTRALETCFCASMGTGPKARGGYDLSLTEADEDFAIQAGSIVGLEVLDQLACAVA
                     SQARIQQDDERIAACAASQTRTIPHASLPHSLYEAHDHSRWDEVAARCLACTNCTMVC
                     PTCFCHGVEETPTLDHQQSEHARVWDSCFTSDHGYIHGKNFRPTTKDRYRMWLTHKLG
                     SWIDQFGMSGCVGCGRCMTWCPVGIDLTEEVHALCQQPS"
     gene            complement(8042..8305)
                     /locus_tag="PKM72_09840"
     CDS             complement(8042..8305)
                     /locus_tag="PKM72_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNP61131.1"
                     /translation="MASYRLTFKKSVPKDFRSIPNNDVSRILKRIEGLADNPRPVGSE
                     KLSGQEKFRVRQGVYRIIYEIRDEELVVIVIKVGHRREVYQDK"
     gene            complement(8292..8525)
                     /locus_tag="PKM72_09845"
     CDS             complement(8292..8525)
                     /locus_tag="PKM72_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61132.1"
                     /translation="MGTSSKRSTVYFDENLHAALRLKAAHTHRSVSDIVNDAVRAALA
                     EDQEDLAAFQQRAGEPTLSYEELLNDLKAHGQL"
     gene            complement(8765..>8967)
                     /locus_tag="PKM72_09850"
     CDS             complement(8765..>8967)
                     /locus_tag="PKM72_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61133.1"
                     /translation="ELEAVDWYNQRANACKDADLKAILAHNRDEEKEHASMVLEWIRR
                     KDPTFSKHLKDYLFTEKPIAHK"
BASE COUNT         2280 a         2444 c         2109 g         2134 t
ORIGIN      
        1 tgataataat ggcatccatg gccgaatgga tgatgcctgc cagttgctgc tcaatcgcct
       61 ccaacccgtt cctggcctcc tgatgcatcg cctggacggc ccgcaactct gccaattcct
      121 gtcggagtag atgaacttcg atctgaaggt cttgaggggg aggctcgtga ctcatggatt
      181 cacctggggt tgaatctgac aaacaggaaa ttaagactta atgcattgat gatacaggga
      241 ccgatgaaga accaccctgg gccggactcc atacgcacat atcactccga tagcagcatg
      301 attcaacgca attcatccga ctgcttgggc gttcacacca cttcctccat ttcagccagc
      361 ttgcgatata aggtctttcg atcaatccct agcacatgcg ccgtttgata tttatttcca
      421 cccatttttt ctaacacccg ccgaatgtag gacttttcca actgctccag gggcagtagc
      481 cgctccgcgg catccccaat gagttgtcca tctccttgga tattccgaat attggaggga
      541 agatcatcca aacaaatctt tttcccttgc gtcagggtcg ccgcgcgctc gaggatattt
      601 tctaactctc tcacattacc cggccactga taatccatga gccgggccat ggccggctct
      661 tcgacacttt caatgtgttt ttgctgcgca gtgacacttt tttgaagcaa cccctgaacc
      721 agaatcggta gatcctcctt gcgatcacga aggggaggca agtgcagttc aatcacattc
      781 aaacgataat acaaatcctc ccggaaccgc ttggccttga cctcctcaac caattgcaca
      841 ttcgtggcga ccactaaccg gacattgatc gacatggagc gagtcgcccc tacccgccgg
      901 atttcccgct cttgtaccgc acgaagtaac ttggcttgaa gcatcatggg catttcacta
      961 atttcatcca aaaacaatgt gccatcgtga gcctcttcaa acaaaccggg tttatccgat
     1021 ttggcatccg taaaagctcc gcgcacatga ccaaagagct cgctttccaa caacagctcg
     1081 ggaatcgccg cacaattcac cggaacaaag ggagcagact tgcgctgact attaaaatga
     1141 atggctttgg caacaagttc ttttcccgtc ccactttctc ccgtgatcag aatattcgtc
     1201 tgtgaatcag ccactcgccg gatcagatca aaaatttcct tcatcggttt acttttcccg
     1261 agaatctgcc caaacccaaa ttcccggctg acctgctgtc gtaactgttt cacttcttgg
     1321 cgaagcagcg cctctcgcat cgccttcgcc accgtgaccg ataattcatt gctcttgatg
     1381 ggcttggtga gatagtcaaa cgcgcctagt ttcatcgcat caacggccga ctccacggtc
     1441 ccaaatgccg tcatcatgat gagattggat tccggatgct ttcgcaggac atgatccaac
     1501 aacgccaggc cgtccatgcc tttcatccgt aaatccgtta aaaccactgg aaacttctct
     1561 gtttccatcc gattgagcgc atcgtgacca tctgaagcca ccatgactcg ataatgctct
     1621 tcctcgagaa tgtcccgaag taattgctgc atttcgggtt catcatccac gactaaaata
     1681 gcaggagaat cgttgatcat tgcaatattc cttctttaac taaccactat ttataaaggt
     1741 aacggactcc gtccaccctg ataaggtata tttccctttg atgacactcc tatctactct
     1801 cgtggtgacc agaggcggca atgtagccac tcctctctgt agcctttttc accaatttgg
     1861 aatttcgcta ctgtagcaat ttgccccaca tgatccaaca cgtctcatcc tattttgatt
     1921 gcgcactaaa gcttcaggga aaattacatc gctatggaga atttgtgatt tcacagaggc
     1981 attcctcttc tgttgcctct aaacaaggta tgacaaggag tacaacttta aaaagggaga
     2041 tcccatgaaa attgtcgttc caattgatgg ttcaacctgt tccacctttg ctgttgaagc
     2101 tcttgcacat tttaagccgc ctgaagaatt gacgctggtc catgcgctac aacttcccga
     2161 tttcaactac ccgatgatta cgccggatct cagaactgaa gcccaggaag agatcaaaac
     2221 ccaattgcga aaagaaggag agggaattct cgatgaggcc caagcacatc ttcctgccga
     2281 tttctcccat gtgcagcggc ttcatcaaat cggacatccg gtcgacgtga ttattgaaac
     2341 agcacagtca gtacaatcca atctgatcct gttgggagcc aggggtcttg gtcccgtcaa
     2401 aaagctgatt ctcgggagcg tgtctcatcg ggttctgatg catgcgccgt gtccaacctt
     2461 gatcgtgaaa acctccatga ctcgcttaca aaaaattctt ctccccatta aaggtcaaga
     2521 ggacgcagac ctcgccctac aatttttggc actccaaccg tttcgacaac cggttgaggt
     2581 cgaggtgttc gcggtctggc cacaacctca gctgtcctgg ccaaccacgg tgggacaatc
     2641 agacgccctg gaagctcaag ccatagaaga agcccaggaa cgcatgaaaa ccatcactga
     2701 ccggctcacc cggatgaact atgcctctca agcccatgtc gggatgggcg atcccgccta
     2761 tgccaccctc gaacaggcca aggcttccca atccgacttg atcatgatgg ggacgcatga
     2821 ccggaaaggg ttctcgcgat ttttgatggg aagcgtttcg aatgccgtgt tacaccaaac
     2881 tccctgcccg gttatgattg tccggtagac gttccgaaac gtgaagaacc gcttcctttt
     2941 tcctactgat gccatctgct acagggctgt tcggacagaa tgaggcgaac tgctacgaag
     3001 gaaatgggac agagcctatt caaggaagga ttttgcagga aacctgtagt cgcttctccc
     3061 atacaactgt tggcatatcc attgctttat atcaggttta ggtatgatcg aaaattttct
     3121 tccaggataa aaaaggaggt tacgagatga gtgccatcac acgttgggat ccttttcggg
     3181 aaatggatga attacaaggt cggctttcaa ccttgttcgg ccgggcaccg gttagaaaag
     3241 aaggggaacg tcaagaagca ttgcgcgtcg ccgaatgggc tcctctggta gatatcacag
     3301 aagatcaaaa agaatatgtg gtgaaagccg agcttcccga ggtcaaaaaa gatgaaatta
     3361 aaatcggcgt acaaaacgat gtgttagtca tttccggtga acgaaaatat gaaaaggaag
     3421 agaaggagaa aaagtaccat cggatcgagc gagcctacgg aagcttttca cgaagcttca
     3481 ccatccctga agatgcggac ccggagaaag tctctgccga attcaaggag ggtattttgc
     3541 aagtgcattt accgaaaagc gaccgggcaa aaccaaaaag tattgccgtg aaggtttcat
     3601 gatcaaacgg gctccccttc tgctttggaa ggggagccct ggttttccgg gatgagcata
     3661 agaccaatca acgcaaagtg agtggaaaag atcactctaa aagaaggaag acaatccaca
     3721 catgcacagt cttggagaat atctatttcg taagtttggg atactttttc ttggattttt
     3781 tctcatagga gggattcctc catccagcgc gcaaccccta caggaagtga aagtgaccat
     3841 agagggatac acgtttcaaa ccacccagat gcctcttcaa ctggacacag agaccattat
     3901 ctacatcaca aacaaagata cggtacgcca tgattttgga tcagacatgt tcctacatac
     3961 tctgacccat gtcgatagca acggcgtcat aacctatggg aaaggggtgg aaggcgtcct
     4021 tcttgagccc ggccaggcgg catcaatcca tttgatgctt aatcattcgg gtcgattcca
     4081 atttcaatgt tcgatccata aggagatgaa aggggaaatt ctcctcctga ccgtggacgc
     4141 cgtataatcg gcaacctctg cattaaccta cgggttgtgg tcgggtgccc gagctattag
     4201 aatttctgag agtgctctcc ggctttcact ctaaggcgaa aggctaatac cgttatggtc
     4261 aagatcgtaa cccatgtatt gtttcctacc gacttttcac caagctccgc ccctgccttt
     4321 cgatatgcgg tggaatgggc aaaagtcttt gaagcccaac tgaccattct ccacgtccat
     4381 tccctgcaac cggggctgga tattgacgcc ggagtcgccc aacaattcct ggatgaacaa
     4441 cgcaaggtgg cgcgggaaga gttggaaaca ctcctggccg aggctcggca acaggttcca
     4501 aaggcatcca tggaactaat ggccggtctg ccgtctgaat gcatttgcga ggttgccagg
     4561 gagaaaaaat gcgatttaat tatgatggga acccacgggt ggaccggaat caatcgcgtg
     4621 ttattcggaa gcgtggcgga acgggtcatt caacgagccc catgcccggt tctgtccatt
     4681 ccgcaccggg aatcagcaga tatctcggcg atgcatcccc tacaaatcct cccacgacaa
     4741 gtcgtcttgc ctttagaatt ctccgattgc tccatggagg cctatgaata tgcgatccaa
     4801 attgcgaaat ggtttgatgt gcctctgacc ctgatccatg ccattgaacc cttgtcctat
     4861 agtttggact ttactctgac tcatccgctt caagaaaaaa ccaatcggga taaggtcgaa
     4921 aaacggttgg ctgatctcac cgccgtcctg accgaacagg gcttatccgc acgatatgaa
     4981 ttactcgata agccaactgt tgacggcatt ctggaaacca gcgccatcca gcaagccgat
     5041 cttattgtca tgggaagtca tggtcgaaag ggactgaccc gaatgattct gggaagcaca
     5101 gcctataagg tgcttgaaca aagcccctac cctgtcctca cagttaaaag tccgaaattc
     5161 gagggagggc atcatccatc gctcgccaat ggcaagacca catcctctcc gacataaacc
     5221 ctgaacctgt gacgatggca gaccatcact ctcaccctat tgccggatcg gaagaaggcc
     5281 ttcgaagtct gttaacccga ttggagcatc aatcgctcca accggacttt cagatacagc
     5341 gacaactggc cctcagccac gcccttcagc cctatttcca tccactcatg gttcctccct
     5401 tggctccttt gccggaagaa gaggacctgg ccaagtggta cctctatgct gattatttgc
     5461 cgactgacgg ccatgcttct ctcatcgagc aagtgcgaga cctggtgacg gaacatgtcc
     5521 cgcaagagga acgggtctgg ctggattcct tgcgacattc ctacatggat ctgctcgagg
     5581 tgcaagagat ggcttccgga aagcagggga ctcagacccg tctgcaatcg ttgggagata
     5641 aacagatatt tgatgttctc ccccacgcga caccggttcc atacaaaatt ggacagatcc
     5701 tgctcaccag gctccttcgg ggattggccg acatacgttt acctggtccc ccactcgttt
     5761 tttcggccag catgggaaaa gccgtcttta aattgaccaa tgagagaaga caggaaatcg
     5821 agattggcac aggtaatttc gccctttcag aatgggcaga atttgccaag agctacgggt
     5881 atgaaatgat gtggagtgtc gccaaggtgc gacgaggagc ctggacagtc gtcgactcac
     5941 agatggacta tcggaatacc caaggcgaaa catttctcta tgccatcgcc ctgtatgaac
     6001 accaggactt ccgcacactg gctgacgggc ttcgtggctt tgatcaattc actcccatac
     6061 aggaaaacgc cggcaagccg gtgtcatccg cttcagggtc cgacacctta acgtgggtat
     6121 ggctcccgcc acagaaccca tccggtgaca caggatcccc tgtcgcccgc ctcaccttga
     6181 cgcccacaca attatgtgtg gaaaccgatg ctccagaccg gctgaatatc gtcaagcatc
     6241 aactggcggc agtatttggc ttctcactcc acttcctggg ggaaaccatt gactcccctc
     6301 agcatcggac acctgaagtt gatctcttat cagacaccta tatccttccg cccaccattg
     6361 tctcccaaga agaagagcaa cagatcctcg cgtcgttgct tgaaaccgtc tatttggatt
     6421 gggccgaaac accttccccg attctgaaag gacaaagccc ccggcatttc tgctctgcgc
     6481 aacaggacac gaaagaggtt gcggccatca tcgaccaaat ggaacaacat gatccgggtc
     6541 ggcgacgcac tggccaaccc gcatacgatt acaacatcct ccggggtcac attggactgt
     6601 aagttaaagg gagtgttgaa aaaggccgcc agcggcgttt tcgccatttg gccgtgctca
     6661 cgtacttctc caaacgctcc gctcgcccaa gtggctgcgg ccttccctgc gagaagcctc
     6721 atgacaggac tggacgggca tttttgaaaa ctcgataacg gttctctggg agcctcggtc
     6781 cctcataggt tgaggaaacg gaaaaaattt taaatattca acagtaattt aaaaggtaat
     6841 catggctacc gaatcttttg aaccttctcc gccagcgctc ctactccgca aggatttttc
     6901 acaactgatt caggttctcc agaaccttgg ctatcggatc ctgggtccgg tcctttctaa
     6961 cggcgcgatt caatgggacg aaattacccg ggaagaagac ctcccgatcg gatggaagga
     7021 ccagcaacat ccaggaaact atcgactgga accagacccc agcgcccggt atttcaatat
     7081 cgttcacggc ccacagtctc tcaagccgct ggtgttttcg ccacgagaaa ccctgttagt
     7141 cctggaccgc aacgaaaaaa cgttttctgc caaagagatc attcctgaca taccacgaac
     7201 cgccgtgctg ggagtgaggg catgcgatct cgcaggactc gggattcaag atcagatttt
     7261 tttgaaaggt ccctatcctg atccctacta tcaacagcga cggaacaatc ttttcctcat
     7321 cgccgtcaac tgcacaaggg cactggagac atgcttttgc gcctcaatgg gcacggggcc
     7381 aaaagcccga ggagggtacg atctcagcct gacggaggcc gatgaagact ttgcaattca
     7441 agcaggaagt atcgttggac tggaagtatt ggatcaactg gcttgcgccg tggcatctca
     7501 agcccggatt cagcaggacg atgaacggat agccgcctgc gcagccagtc aaacgcggac
     7561 cattccgcat gcttcgctac cacatagttt atatgaggcc cacgatcact ccagatggga
     7621 cgaggtggcc gcacgatgcc tcgcctgcac caactgtacg atggtctgcc ctacttgctt
     7681 ttgtcatggg gtggaagaaa ctccaactct ggaccaccaa cagtcggaac atgctcgggt
     7741 ctgggattcg tgtttcacct cggatcacgg atacattcat gggaaaaact tccggcccac
     7801 caccaaagac cggtatcgca tgtggctgac tcacaaatta gggtcatgga tcgaccaatt
     7861 cggcatgtcg gggtgcgtcg gatgcggacg ctgtatgacc tggtgcccgg tgggcatcga
     7921 ccttaccgaa gaagtccacg ccctctgcca gcaaccctcg taaccagttg cgcactggcg
     7981 aaacaaacga gattgacgtg agccgattgc gctttccaat tgattcgaga cgaagcaaaa
     8041 ttcacttgtc ctgatagacc tctcgtctat gccccacttt gatcacgata accacgagct
     8101 cctcgtcccg gatctcataa ataattcgat acactccttg gcgaactcga aatttttcct
     8161 gtcccgacaa tttctcacta ccgaccgggc gtgggttatc cgctaaacct tcaatacgct
     8221 tgagaatgcg agaaacatcg ttgttaggga tggaacgaaa atctttgggg accgactttt
     8281 tgaatgtcag cctataactg gccatgggcc ttgagatcat tcagaagttc ttcataactg
     8341 agagtaggct cgccagcccg ctgctggaag gcagccaggt cttcctggtc ttccgcgaga
     8401 gctgctcgca cagcatcatt gacgatgtca gatacggatc ggtgagtatg cgcagcctta
     8461 agtcttagtg ccgcatggag attctcatca aagtagactg tcgaccgttt agaacttgtg
     8521 cccatggaat ttcacctcct tatgatactg gcattatagc gctttagcgt tataacatca
     8581 gagcgctcaa cgcttaatgg aggttctatc ccaaaaactt ccatggcttc aatgttgaag
     8641 aggccagcat ctggcgccag acgggtattg acctccccga agaagtccac gccctctgcc
     8701 agccacccga atatcaccaa atgcagtgaa tccaggcaga ggtcggtcca atcgacatcg
     8761 ccggctactt gtgcgcgatg ggtttctctg taaacagata gtccttcaaa tgcttcgaaa
     8821 atgtaggatc cttgcgtcga atccattcaa gcaccatcga ggcatgttcc ttctcctcgt
     8881 cgcgattgtg cgcaagaatg gctttgaggt ccgcgtcttt gcacgcattc gcgcgctgat
     8941 tgtaccaatc aacagcctcc agttcct
//
LOCUS       DAORAQ010000109         8864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1255_length_8864_cov_63.176865, whole genome shotgun sequence.
ACCESSION   DAORAQ010000109 DAORAQ010000000
VERSION     DAORAQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1255_length_8864_cov_63.176865"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..3586)
                     /gene="treS"
                     /locus_tag="PKM72_09855"
     CDS             complement(254..3586)
                     /gene="treS"
                     /locus_tag="PKM72_09855"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNP61134.1"
                     /translation="MIVDTLENDPLWYKDAIIYELHVRAFCDSNADGIGDFKGLTQKL
                     DYLQDLGVTAIWLLPFCSSPLRDDGYDISDYTNIHSQYGTRRDFQAFVREAHRRGLRV
                     ITELVVNHTSDQHPWFQRARKAPPGSKWRNWYVWSETPELYSDTRIIFKDTEHSNWSW
                     DPVAKAYFWHRFFSHQPDLNYDNPEVRKAIFGVLDFWLELGVDGLRLDAVPYLFEREG
                     TNCENLPETHAFLKTLRTHVDKKFKNRFFLAEANQWPEDAAAYFGQGDECHMNFHFPL
                     MPRLFMSMQMEDRFPIIDILDQTPAIPESCQWGLFLRNHDELTLEMVTDEERDYMYRV
                     FAHDKQARINLGIRRRLTPLLGNDRKKIELMYSLLFSMPGTPCIYYGEEIGMGDNFYL
                     GDRNGVRTPMQWSADRNAGFSYGNPQKLYLPIIIDPEYHYEAVNVELQQNNAQSPLWW
                     MKRIVSLRKRYKVFGRGSIEFLHPSNRKVLVFLRRYQDETILVAVNLSRHAQWVELDL
                     AEFKGRRPMTLFGRSKFPAIGDLPYLLTLSGHAFYWFALEPVESKQLESQGKTEQGLP
                     TITIPKDWDNLIHKREKVKLENVLPQYLQGRRWFGGKARTMQFVEITEAIPLPQEDPL
                     AVLALIHVEYTEGEPETYLLPLKYLPAEHMAPLLDSPAAIARVRVKMKDGDQEGLLID
                     AMWDREFQKMLLDSISRNRRFTGPVGDLVTQATKIFRRQLQKEVPTLEPTLLKGEQSN
                     SSVLFGHDFILKLYRRAEVGVNPDFEIGRFLTNKGFPHIAPLAGAIEYQRDNGDLLTF
                     GILQKFMQNEGDAWKFTLDELSRYLEEALTHSTAITDSSIPQKSLMALVDEEIPTGAR
                     EWIGPYLEEARLLGLRTGELHAALASDSDDSEFKPEPFTDFYRRGLYQSMLGTVNMNF
                     PLLRTQVKGLQEPVQTLAKHVLEGEGRLRKRLLNIRDRKLTCTRIRCHGDYHLGQVLY
                     TGKDFIIIDFEGEPARPLNVRRLKESPLRDVAGMLRSFHYAALASSIGLVEGVRPEDF
                     SLLEPWARYWQRWVSVSYLKAYLSIKEVRDILPPSSDDIQILLNGYLLQKAIYELGYE
                     LNNRPDWVRIPLDGILQILEVD"
     gene            complement(3770..4633)
                     /locus_tag="PKM72_09860"
     CDS             complement(3770..4633)
                     /locus_tag="PKM72_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNP61135.1"
                     /translation="MEDIVDPTGNMVIRYGLSTFFLFLIIVIVRMGIHHSLFRTTELS
                     VETRRRWAVNLRNGLLFLFILGMIFIWAPQLQTFAVSVFAVAVAFVLATKEIIDCLSG
                     SALRVLTKTYSLGDRIEIGGIRGNVVDHNALTTTVLEIGPGQTSHQYTGRAMVIPNSF
                     IFDHPLTNETYSKKYRLHIVTVPLSTDDDWKTAERLLLQAGEEEAAPFINEARTYLKK
                     LEGKLWLDAPSVEPRVTIQLPEPGRINLLLRVPCPTQYPSRLEQAILRKFLAEFSFAP
                     RLITQETHSTH"
     assembly_gap    4806..4905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4906..5221)
                     /locus_tag="PKM72_09865"
     CDS             complement(<4906..5221)
                     /locus_tag="PKM72_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HNP61136.1"
                     /translation="MERYVVLFGLSLLITGCQDPPIHELQVARQAVENARREGALVFA
                     PDLYSLAESELTIAEEEFHEQSRKVFWARDYSMATRLMMLAQIDAREALSLAQEEKQK
                     TSM"
     gene            5840..6493
                     /locus_tag="PKM72_09870"
     CDS             5840..6493
                     /locus_tag="PKM72_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNP61137.1"
                     /translation="MEGKSESPFNLQLFLTQSGSGKTILTFRQKQTLFSQGDVADAVF
                     YIHSGQIKLSVVSKQGKEAVVGIVESAGFVGEGCLAGQRVCMATATALEDCTLVRISK
                     DAMIRVLHDEPTFSTFFMEHLLARNVRIQEDLVDQLFNSAEKRLARVLLLMAHFGKEG
                     KPEPVLAKVSQEMLAEMIGTTRSRVSFFMNRFRKLGFIEYNGGLHVHSSLLNVVLHD"
     gene            6709..7365
                     /locus_tag="PKM72_09875"
     CDS             6709..7365
                     /locus_tag="PKM72_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNP61138.1"
                     /translation="MTKIVFQETVVMFFLFMAAVAHGSTEGDLPVQNHRLAPCPDSPN
                     CVSSLEDSEKHSIASYRYEKALAEAKAVLKQVFEELSRTELVQEEVISLHTKSEVSCF
                     ALLMMSKFCLMTPQGLFISGRHLGWATPILGLTGSEWRKFEICWRNGSSQVRAHGNNP
                     QPLEGSRETTFSRHISVPACHNAQGEKSAHCLSKVEFPQGWDMVPLSGTVAFRISEQF
                     "
     gene            8042..>8864
                     /locus_tag="PKM72_09880"
     CDS             8042..>8864
                     /locus_tag="PKM72_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61139.1"
                     /translation="MKTLTSSVRGLVTNQDEFQESISADFPFFLERSSGIIKRNLQDL
                     GQWVDDTSHEKSLRRLSFDLVVWFIEYCPSQLPGHPTVLLDGFISDFLERFQESNVSL
                     TSNSILYRFIEGLLRRAAVSRDSLICLFYHFYGMKPAEVGLFLGLEKGETLEIYKGFE
                     RWRQKGWHQAVEEIGLTDKELFSLAETQRSQPEVFHEDVRKILKMLSPFCRKSEPQYN
                     RCVEVEKWYEMFREGYGCEYRMWHLPLCQSCMNTIAKFRDVFWLNATIPSQLHLHI"
BASE COUNT         2087 a         2075 c         2359 g         2243 t
ORIGIN      
        1 gtgacttcca ggatctcccc gacgatccgt ttgaccggat agcgtcccgc atccagttcc
       61 ggttcgacat gttcaatgat cagggtttgt aacgatttag gcatgttgga catgagcact
      121 ccttggtgat tggctttttg actactcgtg aagaggaagg tgtgagtttg tcccccgtca
      181 ggaagatctg atgtatttaa accattgccg ggtgtcagcc ggacgtccgg tttaagccac
      241 cgtttccgga atatcagtca acttctaaaa tttggagaat gccgtccagg gggatacgca
      301 cccaatcggg ccggttattg agttcatagc cgagttcata gatcgctttt tggagtaaat
      361 agccattcag caaaatttga atatcgtcgg aggacggggg cagaatgtca cggacttcct
      421 tgattgacaa atacgctttg agatagctga cgctgaccca tcgttgccag tagcgagccc
      481 aaggttcgag taaggaaaag tcttccggtc gaacaccctc gaccaatcca attgaggagg
      541 caagcgccgc ataatgaaag gatcgcaaca tgcccgcgac gtcgcgcaac ggagattcct
      601 tgagtcgtct gacattcaat ggtctggccg gttcgccctc aaaatcaata atgatgaaat
      661 cttttccggt atagagcacc tgaccgagat ggtagtcgcc atggcatcgg atgcgggtgc
      721 aggtgagttt ccggtcccgt atgtttaaga gacgtttgcg taagcggcct tctccttcca
      781 gcacgtgttt tgccagtgtt tgcacgggtt cctggagccc tttgacctgg gtgcgaagaa
      841 ggggaaagtt catgttcacc gtgcccaaca tgctctgata caagccccgc cggtaaaaat
      901 ccgtaaatgg ttccggttta aattcggaat catcagagtc ggaggcgagc gcagcatgca
      961 gttctcctgt tcgaaggccc aacaaccgtg cttcttccag atagggacca atccattcgc
     1021 gggcgcccgt cggaatctcc tcgtccacca gggccatgag agatttctga ggaattgacg
     1081 agtcggtgat ggctgtggag tgggtgagcg cttcttccaa gtatcgactc agttcatcca
     1141 acgtgaattt ccaggcgtct ccttcatttt gcatgaattt ctgtaagatg ccaaacgtca
     1201 ggagatcgcc attatcccgt tgatattcga tggctccagc caggggcgcg atgtgcggaa
     1261 atcctttgtt cgttaaaaac cgtccgattt caaagtcagg attcacgccg acttcggccc
     1321 gtcggtagag cttgaggatg aagtcatgtc caaacaaaac cgaggagttg ctttgttcac
     1381 ctttgagcaa ggtgggttcc agtgtcggga cctctttctg aagttgccgc cggaaaattt
     1441 tcgtggcttg ggtgaccaaa tcgcccactg gaccggtaaa tcggcgattt cgggaaatac
     1501 tgtccaatag cattttttga aactcccgat cccacatggc atcgatgaga aggccttcct
     1561 ggtcaccgtc tttcattttg actcgcaccc gtgcaatagc agccggagaa tccaacagtg
     1621 gggccatatg ttcggccggg agatacttca agggcagaag gtatgtctcc ggttctcctt
     1681 cggtgtattc cacatgaatg agggcgagga cggccaaggg atcctcttgc ggaaggggga
     1741 tcgcttccgt gatttccacg aattgcatgg tgcgggcttt gcccccaaac caccggcggc
     1801 cttgcaggta ctgaggtaac acattttcga gtttgacttt ttcccgttta tggatgaggt
     1861 tatcccaatc ttttgggatg gtaatggttg gtaagccctg ttcggtcttc ccctgggact
     1921 cgagttgttt tgattcaacc ggttccaggg caaaccagta aaacgcgtgc ccgctcaggg
     1981 tgagcagata aggcaaatcg ccgatggccg gaaatttact ccgtccgaac aaggtcatgg
     2041 gacgtcgtcc tttgaattcc gcgaggtcga gttcgaccca ttgggcatgg cgggataagt
     2101 ttacggcgac caggattgtt tcgtcctgat aacgccggag gaaaaccagg actttccgat
     2161 tggagggatg gagaaactca atggatccac gtccgaagac cttatagcgt ttccgcaatg
     2221 agacgatgcg cttcatccac cagagagggg attgggcgtt attttgttgc agttcgacat
     2281 tgacggcttc gtaatgatat tcggggtcaa taataattgg caggtagagt ttttgcggat
     2341 tgccatagga aaatccggca ttgcggtcgg cactccattg catgggagtg cggaccccgt
     2401 tccggtcgcc taaataaaaa ttgtcgccca tcccgatctc ttccccgtaa taaatgcatg
     2461 gagtccccgg catcgaaaat aacaggctgt acatcagttc aattttttta cggtcattcc
     2521 cgaggagcgg ggtcaagcgt cgacggatcc ccaaattgat cctggcctgc ttgtcatggg
     2581 caaagacccg gtacatgtaa tcccgttctt catccgtcac catctccagg gtgagttcgt
     2641 cgtgattgcg caaaaagagc ccccattggc aggattccgg aatcgcaggc gtctggtcca
     2701 aaatatcgat gatgggaaag cgatcttcca tttgcatcga catgaatagg cggggcatca
     2761 agggaaaatg gaagttcatg tggcattcat cgccctgccc gaaataggct gcggcgtctt
     2821 ccggccattg attggcttcc gcgagaaaaa accggttttt gaatttttta tcgacgtgtg
     2881 tcctcagcgt tttgagaaac gcatgggttt ccgggaggtt ttcgcaattg gtcccttccc
     2941 gctcgaagag ataaggcacg gcatccagtc gcaagccgtc cacacccaat tccagccaga
     3001 aatccaggac cccaaagatt gcctttcgca cctcggggtt gtcgtaattc aggtccggct
     3061 gatgagaaaa aaaacgatgc cagaaatacg ctttggccac gggatcccag gaccagtttg
     3121 aatgttcggt atctttgaaa ataatgcggg tatcggaata cagttcaggg gtctcactcc
     3181 agacgtacca attccgccat ttactgccgg ggggagcttt ccgtgcgcgc tgaaaccagg
     3241 ggtgttggtc tgaggtgtga ttgaccacta attctgtaat cacccgtaat ccacggcgat
     3301 gggcctcccg gacaaacgcc tggaagtcac gcctggttcc atactgtgaa tgaatatttg
     3361 tgtagtcgga aatgtcatag ccatcgtccc ggaggggaga tgagcaaaag gggagaagcc
     3421 agatcgcggt cacacccaga tcctggagat aatccagttt ttgggtcagc cctttgaaat
     3481 cgccgatgcc atcggcgtta ctatcgcaaa atgcccgaac atggagttca taaataatgg
     3541 catctttgta ccacagtgga tcattctcga gtgtgtcgac aatcatggat aggcctttcc
     3601 tcaatcctgt ctatactaat tgtttcccgg ccgtacacag gaagtgcggt ctcgatatgg
     3661 gttgtgatgt cgcaatattt ggctggacgt ctgttcagcg agcatgctga aaaagataaa
     3721 cgagtaacac tgatttacca tataaatgga tggaaatgcc aatccattgt taatgggtgg
     3781 aatgcgtttc ttgtgtgatg agacgggggg caaaggagaa ttcagcaagg aattttcgaa
     3841 ggatggcttg ttccaggcgt gaaggatatt gagtggggca ggggacgcgg agtaataaat
     3901 tgatgcggcc gggctcaggc aactgaatgg tgacccgtgg ttcaacggag ggggcgtcta
     3961 accacagctt gccttcgagt tttttgagat aggtccttgc ctcattgata aacggggcgg
     4021 cctcttcttc tcctgcctgt agcagcaggc gttcggcggt tttccaatca tcatcggtac
     4081 tcaacggaac cgtcacaata tggagccggt atttttttga ataggtttca tttgttaggg
     4141 ggtgatcgaa aataaagctg ttgggaatga ccatggcccg gccggtatat tgatgagagg
     4201 tttggcctgg tccgatttcc agaaccgtgg tcgtcagggc gttgtggtca accacgtttc
     4261 ctcgaatacc tccaatttcg atgcgatcac ccaacgaata ggttttggtc agcactcgaa
     4321 gtgccgatcc gctcaagcaa tcaatgattt ccttggtggc caggacgaag gccacggcga
     4381 cagcaaacac ggagacggca aaggtttgga gttgcggtgc ccaaatgaaa atcatcccga
     4441 gaataaacag aaagagcagg ccatttctga gattgaccgc ccaccgtctc cgtgtttcga
     4501 cggaaagctc ggttgtccgg aaaagactgt gatggatccc catcctgacg atgacaatga
     4561 ttaaaaagag aaagaacgta ctcaggccgt accggatgac catgttgccg gtcggatcta
     4621 caatgtcttc cacgggtggc tcctcaagac tgatgtatga attggacggg ttgagatcaa
     4681 cagaaacatc atgagagtct caggcatcat tccaacctga tcgattggga aaaggcgaaa
     4741 gtccaagagc tggcagatga acgaaataca ggtgaatcac aatccaacgg gtcaagctga
     4801 aggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacatt gaagttttct
     4921 gtttttcctc ttgtgccaga gagagagctt cacgggcatc gatttgtgcc aacatcatta
     4981 accgggtggc catggaataa tcccgagccc agaatacctt tctggattgt tcatgaaatt
     5041 cttcttccgc gatagtcagc tcactttccg ctaaactgta gagatcaggc gcgaagacca
     5101 gggctccctc tctcctggca ttttccaccg cttgacgggc tacctgcaat tcgtggatgg
     5161 gtggatcctg acagccggtt attaaaagac tcaagccgaa caagaccaca taccgttcca
     5221 cacgggagag tctccaagcc ctcgcaacac atttccttat cagggggttt tcttctaaca
     5281 aaatggacct tggttttgaa acattttcga gaaggccgga agcgcatctc catcgcaatc
     5341 aaagtctgat gtagcaaatc ctacgccaat ggtgaatgaa gaaggctgtt ggtagatgcc
     5401 cgatttttca gttgtttttc aggagatggt ttctgggttt gggacacctt gggctcctgc
     5461 ttggaacctt tgtccggatc tgattttgga aggagaagga gtcacagggt gtggtgattc
     5521 ttcaccggga agggccggtt gccatgggga gggggagggg ttttcctgag gtgccaggga
     5581 aatctgtggg tctgcttgta ccttggcatt ttcagagtgg aatggcctca aatgaccagg
     5641 ggggtgtggg tagaacggtt ggcctaaagg ttggccctat cgtcccagga aagactcctg
     5701 ggccctcgct tcaggcttca agaaatgtct tgctcaaatt attgtattta gggaataata
     5761 ttttgattga ccaggttttt gctcgaatct cccccttcct tataaaaatt ctacttctgg
     5821 ctctcaaagg aggcagccta tggaaggcaa gtcggaatcg ccctttaatc tccagttgtt
     5881 cctaacgcaa agcggtagtg ggaaaacaat tttgacattc cgtcaaaagc agacgctctt
     5941 ttcgcaaggg gatgtcgcgg atgctgtgtt ctatattcac tccggccaga tcaaactttc
     6001 ggtggtctcg aagcagggga aagaagccgt ggtcgggatc gtggagtctg cggggtttgt
     6061 aggggaaggt tgcctcgccg ggcagcgggt gtgcatggcc acggcgacag ccctggagga
     6121 ctgcactctg gttagaattt ccaaggatgc gatgatccgc gtgctccatg acgaacccac
     6181 cttctccacg ttcttcatgg aacatctcct ggctcgcaac gtccgtattc aggaggactt
     6241 ggtggatcag ttgtttaatt cggctgagaa gcggctggcg cgtgtgctgc tgttaatggc
     6301 ccattttggg aaagagggca agccggaacc ggtccttgca aaggtcagtc aggaaatgct
     6361 tgccgagatg atcggcacga cgcgctcccg cgtcagtttc ttcatgaata gattcagaaa
     6421 attgggtttc atcgagtaca acggcggttt gcatgtgcac agctccctcc ttaatgtcgt
     6481 cctccacgac taggattttc ctttattttc gcggcatcag atgcgtacct cccttcgtta
     6541 attttcggcg gcatgcacgg tttttccctc tagactgagc aattttggtc agaattctgt
     6601 aggttatcct gccagaatga acttcgctat tggccaggac caatcctcgg tcggcccgaa
     6661 atctgactta tctaggagcc tatcgtcgga tatcagggga atgctctcat gacgaaaata
     6721 gtttttcagg aaacggttgt gatgttcttc ctattcatgg ccgcggtggc tcatggatca
     6781 actgaggggg acttgccggt gcagaatcat cgactcgccc cgtgccccga cagccccaat
     6841 tgcgtatcca gcctcgagga ttcggaaaaa cattccatag catcctatcg ctatgagaaa
     6901 gcgctggctg aggccaaagc ggtgttgaaa caggttttcg aggaattgag tcgcaccgag
     6961 ttggttcagg aagaggtgat ctcccttcat acgaagtccg aagtttcctg tttcgctttg
     7021 ttgatgatgt cgaaattctg tttgatgacg ccacaaggac tattcatttc cggtcggcat
     7081 cttgggtggg ctactccgat tttggggtta accggaagcg aatggagaaa attcgaaatc
     7141 tgctggagga acggctctag ccaggtgcgg gctcatggaa acaatccgca acctctggag
     7201 gggagccgcg aaacaacctt ttctcgccac atcagcgtcc cggcttgcca taacgcgcag
     7261 ggagaaaaaa gtgctcattg cctttcaaaa gtggaatttc ctcagggttg ggatatggtg
     7321 ccactcagcg gcactgtggc ctttcgcatc agtgagcaat tttgaccaga tcttatgctg
     7381 tctctttgct atggtttgaa tggactaaca aacgcaaccc ctctcgcatc cttaactgta
     7441 ggaggagacg ttatgacctg ccgatctgct cgactgtttt taaaccctgc ccccctgaat
     7501 ctggtctact gccgtatgtg tggctcgacc gatttggaac tgtcctgttc atcaaaaatc
     7561 tttggaccac acatgtgttt gatttgtgtc cagtccctga ttgagttggg aacggcaaag
     7621 caatgtggtc ctgtgccgtt gcgtgaactt gtacgttcct gagctaagtg ccgcacggga
     7681 gtctggccgg acccactggg cgcttcattg aacccccggt ggcggcgtgg ccgcttctcc
     7741 ggtccgaagc aaccgcggtc acgttctcat ttaaattctt gcaatctgtt caatattgaa
     7801 cagacaggga gaggccttcc cgccatactg gccaatcatc tgaagatgag ggatgccaga
     7861 atgttcaaaa accggaacag gtatcaccga accagatctc atttcagaaa agtccaacat
     7921 catgcatcca cgtgtgcgtg gaatgagcac ctctgtgatc tcgaaacagg acggtaaagg
     7981 tgaaggggct ccgaacacca tggattaata tatggtctta tggggtcgaa aggagaacgc
     8041 tgtgaaaacg cttacttctt cagtcagggg gctggtgacg aatcaggacg aattccagga
     8101 aagtatttcg gccgattttc cgtttttcct ggaacggtca tcgggcatta tcaaacgaaa
     8161 tttacaggac ttggggcaat gggtcgacga cacctcacat gaaaaatcgc ttcgcaggct
     8221 gtcctttgac ctggttgtct ggtttattga gtattgccca tctcaattgc cgggtcatcc
     8281 tacggtgttg ttggacggct ttatttcgga tttccttgaa agattccagg aatcaaacgt
     8341 ttcgctgacc tccaattcca ttttataccg attcattgaa ggactgctcc gtcgtgctgc
     8401 ggtaagccgt gattccctca tctgtctgtt ctatcatttt tatggcatga aacccgccga
     8461 ggtgggtctt tttctaggat tggaaaaagg agaaacgctg gagatctata agggtttcga
     8521 acggtggaga caaaaaggat ggcaccaggc ggttgaagaa atcggattga ccgacaagga
     8581 attgttctca ttagccgaga cgcaacggag tcaacctgag gtcttccatg aggacgtccg
     8641 aaaaattctg aaaatgttat cgccattctg tcgtaaaagt gaacctcagt ataaccggtg
     8701 tgtggaagtg gaaaaatggt atgagatgtt cagggaaggg tatggatgtg aataccggat
     8761 gtggcattta cccttatgcc agtcttgtat gaacacaatt gccaagttta gagacgtatt
     8821 ttggctcaat gccaccatac catcacaact ccatctgcat atct
//
LOCUS       DAORAQ010000110         8849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1258_length_8849_cov_67.284057, whole genome shotgun sequence.
ACCESSION   DAORAQ010000110 DAORAQ010000000
VERSION     DAORAQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_8849_cov_67.284057"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PKM72_09885"
     CDS             complement(<1..772)
                     /locus_tag="PKM72_09885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNP61140.1"
                     /translation="MDSTTPPSKVYVLKRPLEESTPPKLSRNYAEELNPQQLAAVEAV
                     EGPALVIAGAGSGKTRTLVYRVARLIDLGIVPSSILLLTFTRKASQEMLSRVGLLIGL
                     RAQQVGGGTFHSMANILLRRYGRPVGLEPGFTILDRGDSEDLLSLLRGQLGLNDTGKR
                     FPRKHTLSEIFGKTANTLESLENIVINEYSHFGEYLGELQKLQAAYEAAKRQRQLVDY
                     DDLLVKLLELLVIDQPTRETISQIFRYILVDEYQDTNRL"
     gene            937..1836
                     /locus_tag="PKM72_09890"
     CDS             937..1836
                     /locus_tag="PKM72_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61141.1"
                     /translation="MAKQSAENYENVLKDLAHTMMSVARESVGLIKRSDQKLAMKLQR
                     KQEWEIYLEFLRMLFNLVDRLSGFYIPIQAQKEFMNSLEDSVAHQLKTVLAPSLSSSE
                     VDDMEVTLSVGQVVADSRKTYEKFKFVVTDQSKERDQYFQFFSERIASKAGAPGKQEI
                     TAAAFLCGSAVVPALKSLFEDATQPKVEPATSAVPPAAKEGAGSSAAAQEGSSSTKSG
                     GTDTAGKNLIKLISVVSRAQGEEVESWWGLHPQFRRDLPEDEAKELAKHMNRATRIVG
                     ERFAVVASLAQSETNPNQPVGNA"
     gene            1948..4104
                     /gene="glgP"
                     /locus_tag="PKM72_09895"
     CDS             1948..4104
                     /gene="glgP"
                     /locus_tag="PKM72_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNP61142.1"
                     /translation="MNQSTRLLTEEFRNLSELAHNVWWSWSPEGRAVFSYIDPTLWRL
                     TYHDPIKQLQKIAPDRLDVLGQDVVFLSLYREAMKAFHAYMETKDHWFGRTYPQWQDR
                     TIAYFSAEFGLHRSVPLYSGGLGILAGDHLKEASDLGVPLVAVGFMYSQAYFRQVVDT
                     NGWQEAVYDSVDPMMMPIELARTPAGDLAKIQVQLGTRMVACVIWQIQVGRVSLYLLD
                     TDTPENSPEDRHLTARLYGGDHRTRLCQELLLGIGGVRALRAVGHDPNVWHANEGHPA
                     FFLVERMRELLQQGLSWSEAADQIRQSTVFTTHTPVPAGHDVFSGDLIREHCHWWWEE
                     LGLTQENFMALGRHPELSSDQFHMTTLPIRLASFINGVSKEHELVSKKMFHILWPERP
                     LQEVPIHSVTNGVHVPTWIAQEMDVLYQKYLGSDWRERCDDPNMWQRMKEVPDEEIWE
                     VRKFLKRKLMSFIRQRARMGWMDGTMEPIQVLASGAFLEPHALTLGFARRFATYKRAT
                     LLFSDLERLKQILLNPWRPVQIVFAGKSHPADQAGRELIHRIYQFAKAHHLGGQIAFV
                     EDYDMHVAKFLVQGVDVWLNNPRPPLEASGTSGQKAALNGVPNLSVLDGWWKEGYDGS
                     NGWAVPLPESPLGDWAQDDLDMVGLYTLLENDVIPLFYDRGVDGIPHGWCTVVKNSIR
                     TSAPRFSARRMLKEYVKRAYTPLFSEAGNARDEKLA"
     gene            4364..4789
                     /locus_tag="PKM72_09900"
     CDS             4364..4789
                     /locus_tag="PKM72_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61143.1"
                     /translation="MKVLRILILLGIVFGLGYYTGQKPDEVKQRLRDLSGQVLENTIG
                     FDQKTHLQRQMLAAKDGFLEGRAYLLDHQFKEASVEFERALHHLDQAVELDPQGPMAQ
                     KIRMVKQKIREAQQSLAQGHSLPPHSLDDLRQEMQSVMP"
     gene            complement(4997..5869)
                     /locus_tag="PKM72_09905"
     CDS             complement(4997..5869)
                     /locus_tag="PKM72_09905"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP61144.1"
                     /translation="MRDDQKNMDDAFSDKTEEFVSLEGKTTLPQDELVDEVSEAITAD
                     ADEETTDIVASAEETEEETEQEEELVEEQVKDEIDIQIDLLNDPEWVVRREAVTTLGE
                     MGDERCIEPLVRCLRDGDWQVRDAAVEAIAMIGSPAVDLLLRYIRDYDARKSVIKALG
                     KINDERVLDPLISMLHNDEFKDDATWALAELGQPAVGRLLECLKNQDEVIRKQAILAL
                     GEIRDSSSVDLLMEGLQDPDWFIRLSSAAALEKIGDPRGREAIKLLAKDPDLVVRLRI
                     EHMLAEWKKQAVTA"
     gene            complement(5914..6117)
                     /locus_tag="PKM72_09910"
     CDS             complement(5914..6117)
                     /locus_tag="PKM72_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61145.1"
                     /translation="MAKRRSLQEDATSLKTKVTKSLASSDNPEGDSTIRSLRKRLRRV
                     QRKVRTAKRREEQRKSKKVVAEA"
     gene            complement(6319..6780)
                     /locus_tag="PKM72_09915"
     CDS             complement(6319..6780)
                     /locus_tag="PKM72_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP61146.1"
                     /translation="MTFSMKVLGTLTAAMFLTTGLAIAGPEKDPIPSRVPADQRSAAK
                     KDKTDLYSKAEDASPEIVAEGKALYEGKGTCINCHGKEGNGQGPAGAVLTPGPRDFTN
                     CKFHKKRKDGELMWVIKNGSPGTGMVPLVPATISEEEAWKVIAYERSFCKD"
     gene            complement(7198..8070)
                     /locus_tag="PKM72_09920"
     CDS             complement(7198..8070)
                     /locus_tag="PKM72_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNP61147.1"
                     /translation="METLGGLFRQTRERQRLSLEQIASKTRIQQRHLQALEEEDFASL
                     PAKVFVKGFVRSYARSLGLDEDNALQLFLTTSSDFYDRTQEEQQHIQVTLQAAHRKRF
                     NWNLVVILFLAIGGVLFYLLPWQQETVPLPPESETPEPISGMQNKEILDPTPHIEELP
                     DSVSSEFPVDTEIQLTSPPPPDIIPSQLPPEPRPVPTLPVSPVPEKATGIDGTLVLEI
                     EATQLTWVVVQSDDQAPHEALLQPGQKSTWKADKQYLLTLGNAAGVIVRLNGELQGPF
                     GKPGQVVRDIHLKP"
     gene            complement(8170..>8849)
                     /locus_tag="PKM72_09925"
     CDS             complement(8170..>8849)
                     /locus_tag="PKM72_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNP61148.1"
                     /translation="RWRGEYSYQLNEAQTLETHLNVLETFRPKIPASYTTPSALFLGN
                     IDPELQLDVLNQVKRPSIVACDTMNFWIEGKREALWKVLEHIDILVINDGEARALGDD
                     ANLVQVAKRILSRGPKTLIIKRGEYGVLMFHQQEIFGAPAYPLEAVKDPTGAGDTFAG
                     GFMGYLSATENYSQAGLRQAIIFGSVMASFNVEEFSLDRLRHLTREDIDKRFSSFKHL
                     THFEDLS"
BASE COUNT         2029 a         2024 c         2386 g         2410 t
ORIGIN      
        1 gcaggcgatt ggtgtcttga tattcatcca ccaaaatata gcgaaagatc tgactgatgg
       61 tttctctggt cggttggtcg atgaccagga gttcaaggag tttgaccagg aggtcgtcgt
      121 aatccacgag ttgccgttgg cgcttggcgg cctcataggc ggcctggagt ttctggagtt
      181 ctccgaggta ctccccgaag tggctatatt cgttgatgac aatgttttcc aggctttcca
      241 gtgtgttggc cgttttgccg aatatttcgg atagggtatg tttgcgcggg aaccgtttgc
      301 cggtatcatt cagccccaat tggccacgga ggaggctcag gagatcttca gaatctccac
      361 gatcgagtat cgtaaacccc ggttccaggc cgacgggacg gccatatcgt cgaaggagga
      421 tattagccat ggagtgaaag gtcccaccgc cgacttgctg ggcgcggagt ccgatgagga
      481 gtccgacccg gctgagcatt tcctgtgagg cttttcgggt aaaggtcagg agtaaaatgg
      541 agctgggtac aatgcccagg tcgatgagcc tggcgacccg gtacaccagg gtgcgtgttt
      601 ttccgctgcc ggcgcccgca atgacgaggg ctggaccttc cacggcttcc actgcggcga
      661 gttgttgggg atttaattct tccgcgtaat tacgcgataa ttttggtgga gtagactcct
      721 ccagtgggcg tttcaggaca taaactttgg atggtggcgt agtggaatcc atgaatccat
      781 gggcaggtat tgacgggaga taagaacccg agtttcgtct ttcgaagacc tttggccgtg
      841 tatagcatac gtggtggtgg gttgcaacgc gaatgatatt aggcttggac aggtcttcta
      901 tcctcagtta tactttactc aattagggag ttaaacatgg ccaaacaatc tgcggaaaat
      961 tacgagaatg tgctgaagga cctggctcat acgatgatga gcgtggctcg ggaatcggtc
     1021 ggtctgatca agcggtccga ccaaaagcta gccatgaagc tgcagcggaa gcaggagtgg
     1081 gagatttatt tagaattcct gcgcatgctg tttaatcttg tggatcgact gtctgggttt
     1141 tatattccga ttcaggccca gaaggaattt atgaatagtt tggaagattc cgtggcccac
     1201 caattaaaaa ccgtcctggc cccctctttg agttcgagtg aagtggacga tatggaggtg
     1261 acgctgtctg ttgggcaggt ggtagccgat agtcggaaaa cctatgaaaa atttaagttt
     1321 gtcgtcacag accaatccaa agagcgggat caatatttcc agtttttttc ggaacgaatt
     1381 gcctctaaag ccggagcccc gggcaaacag gaaattaccg ctgcggcatt tttgtgtgga
     1441 agcgcggtcg tgccggctct caaaagctta ttcgaagatg cgacccaacc caaagtagaa
     1501 ccggcaacgt ctgcggttcc cccggcggcg aaggaagggg ccggttcatc ggcggcggct
     1561 caagaaggca gttcttcaac gaaatcaggt ggcacggata cggctggcaa gaacctgatt
     1621 aaattgatca gcgtggtgtc tcgcgcacag ggggaagaag tcgaatcctg gtgggggctc
     1681 catccacagt ttcgccgcga ccttccagag gatgaagcca aggaattggc caagcacatg
     1741 aatcgggcga cccggatcgt gggcgagcgg tttgcggttg tggcttcctt ggcacaatcg
     1801 gagacgaatc caaatcaacc tgttgggaat gcctaaccct ttctaactga ctcttctgag
     1861 tgttctcttt taccacttca acatgtgttc atggttggtg aagatccttc accatcggat
     1921 gatgtcatct ttttgtgagt gtaccctatg aatcaatcaa ctcgtttgtt gacggaagaa
     1981 tttcgcaacc tttctgagtt agctcataat gtctggtgga gttggtctcc tgaaggccgg
     2041 gctgtatttt cttatattga tcccactctc tggcgcctga cgtatcacga tcctattaaa
     2101 cagttgcaga agattgcccc tgatcggttg gatgttctcg ggcaagatgt ggtgtttctt
     2161 tccctgtatc gcgaggcgat gaaggcgttt catgcttata tggagaccaa ggaccactgg
     2221 tttgggcgaa cgtatcctca gtggcaggat cgcaccattg cctatttttc ggcagaattt
     2281 ggattgcatc gttcggttcc cctgtatagc gggggactag ggattctggc tggtgaccat
     2341 ctcaaagagg ccagtgacct gggagttccg ttggtggctg tcgggtttat gtatagccag
     2401 gcctatttcc ggcaggttgt ggataccaac ggttggcagg aagcggtgta tgattccgtc
     2461 gatccgatga tgatgccgat tgagttggcg cggacgccgg ccggggattt ggcaaagatt
     2521 caagtccaac tgggtacccg aatggtcgcg tgtgtgatct ggcaaattca agtgggccgg
     2581 gtttcgcttt atcttttgga taccgataca ccggaaaatt caccagagga ccgtcatctg
     2641 actgctcggc tttatggagg ggatcatcgc acacggcttt gtcaggagtt gttgttgggc
     2701 atcggcggtg tacgggctct tcgtgcggtg ggccatgatc ccaatgtgtg gcatgccaat
     2761 gaaggacatc cggctttttt tctggttgaa cggatgcggg aactacttca acaagggctg
     2821 tcctggtcgg aggcggccga tcagattcgt cagagtaccg ttttcacaac ccatacgcct
     2881 gtccctgccg ggcacgatgt cttttcgggg gacctcattc gcgaacattg tcattggtgg
     2941 tgggaagagt taggcctcac acaggaaaat ttcatggcgt tgggtcgtca tccggaatta
     3001 tcatctgatc aattccatat gaccacattg cctatccgtc tagcctcatt cattaatggc
     3061 gtgagcaagg aacatgaatt ggtctcaaaa aaaatgtttc acattctgtg gccggaacga
     3121 ccgctgcagg aggtgcccat ccacagcgtc accaacgggg tgcatgttcc cacgtggatt
     3181 gctcaggaaa tggatgtgtt gtatcaaaaa tatctgggat ctgactggcg ggaacggtgt
     3241 gacgatccca acatgtggca gaggatgaag gaggtgccgg atgaggagat atgggaggtc
     3301 cggaaatttc tgaaacgaaa gctgatgagt tttatccgtc agcgcgccag gatgggatgg
     3361 atggatggga cgatggagcc tatccaggtg ttggcgagcg gggcgtttct tgagccgcat
     3421 gcgttgactc tgggatttgc gcgacggttt gcgacctaca aacgggccac gctgttgttt
     3481 tcagacctgg agcggctgaa acagattctg ttgaatccgt ggcggcctgt tcaaattgtt
     3541 ttcgccggaa aatctcatcc ggccgaccag gcagggcgcg aactcatcca tcgtatttat
     3601 caatttgcca aagcgcatca tctgggcggc caaattgcgt ttgtcgaaga ttacgacatg
     3661 catgtggcaa aatttttggt gcaaggggtc gatgtctggc tcaataatcc tcgcccgccg
     3721 ttagaggcga gcgggacaag cggccagaaa gccgctttaa atggcgtgcc caatttgagc
     3781 gtgttagatg ggtggtggaa agaaggctat gatggcagta acggctgggc agtgcctctc
     3841 cctgaatcgc cacttggcga ttgggcccag gatgacctgg atatggtcgg cctctacacc
     3901 ctacttgaaa acgatgtaat cccgttgttt tacgaccgtg gtgttgatgg cattccacat
     3961 gggtggtgca ctgttgttaa aaactcaatt aggacatcgg ctccacggtt cagtgctcgc
     4021 cgcatgctca aagaatacgt gaagcgtgcg tataccccgc tgttttccga ggcagggaat
     4081 gcacgagacg aaaaactggc ctgaatctct cagcgtggtc tgaaaatttg tgtctgatgc
     4141 gtgataggcg atttgttgcg cagaagttca tggacacccg tatcacagtt gtaccttctg
     4201 caatgcagtg ataccatccg cttacgtatg gtgcaccatt ccctttctca tcggtatctc
     4261 gtcgatcgat atgatttttt cttccagtct aatttatgga gtgaaatagg tttgaggtcc
     4321 attcaacaaa gagcgagaat cttctgagcc ggcggggtcc actatgaaag ttttacggat
     4381 attgattctg ctgggtattg tatttggatt gggctattac acggggcaaa aaccggatga
     4441 ggtgaagcaa cgattgcgtg atttgtcagg gcaggttttg gagaacacga ttggatttga
     4501 tcagaagacc catttgcagc gacaaatgtt ggcggcaaaa gacggctttt tggaaggccg
     4561 agcgtatctc ttggatcatc aattcaaaga agcttcagtt gagttcgaac gtgcacttca
     4621 ccatttagat caagcggttg agttggaccc tcaaggcccc atggctcaaa aaatcagaat
     4681 ggttaaacag aaaattcgag aggctcaaca aagtctggct caagggcata gtcttcctcc
     4741 tcattcgttg gatgatcttc gtcaggagat gcaatctgtt atgccttgac gaaacctacc
     4801 tatagcaact gccagttgcg ttccttgttt tttctcgtcc catcttgtca gaattattta
     4861 attccaggtt attacttgat gggaacctta aatggaattt aagaaacagg gtgggttttc
     4921 ctcataaaaa gccccctcca tcgtccaggc gatggagggg gctggtatgt tcacaataag
     4981 gactggaaag tcaggcttaa gccgtaacgg cctgcttttt ccattctgcc aacatgtgtt
     5041 cgattcgtaa gcgaaccacc aagtcggggt ctttggctaa aagtttaatc gcctctcggc
     5101 cacgaggatc tccgattttt tctagcgcgg ctgctgacga aagccggatg aaccagtcag
     5161 ggtcttgcaa tccttccatt aataaatcaa cactactgga atccctgatc tctcctagag
     5221 ccagaatggc ctgtttccta ataacttcat cttgattctt caggcattca agtaatcgtc
     5281 caacagccgg ttgtccaagt tctgcgagcg cccaagtcgc atcgtcctta aactcatcat
     5341 tgtggagcat cgaaattaac ggatcaagga ctcgttcatc gttaattttc cccaacgctt
     5401 taatcactga tttccgggca tcgtagtctc gtatatagcg gagcagaagg tccacggccg
     5461 gtgaaccaat catggcgatg gcttcaactg cggcgtctcg aacctgccaa tctccatctc
     5521 gaaggcatcg aaccagaggt tcgatgcagc gttcatcacc catttcgccc agggtagtca
     5581 ctgcttcgcg tcggacgacc cattctggat cgttcaaaag atcgatctga atatcgattt
     5641 catctttgac ctgttcttcg accaactctt cttcttgttc tgtttcttct tcggtttcct
     5701 cagcagaggc aacgatgtct gtggtttctt catctgcgtc cgccgtgata gcttctgaga
     5761 cttcatccac cagctcgtct tgaggcaggg ttgtcttgcc ttccaacgat acaaactcct
     5821 ccgttttatc tgaaaacgca tcatccatat ttttttggtc gtcccgcatg cctcaattcc
     5881 tccaaattct cttcagggtc aatctgcaga accttaggcc tctgccacga ctttctttga
     5941 tttccgctgt tcctcgcgcc gtttggctgt tcggaccttc cgttgcactc ttcgtaaacg
     6001 ttttcgtaat gatcggatgg ttgaatctcc ctcaggattg tcgctactgg ctagggattt
     6061 cgtgaccttg gtttttagag acgttgcgtc ttcctgaagt gatcgtcgtt tggccatatg
     6121 aacatgctct ccttactcta gaaattttat tgacgaaaag tgatttgggt ggccttctac
     6181 ctggttgagt ctagtcaagg aatttgaatg gtgtcaacgg ggaaacgcta tacttggcag
     6241 ggactaaggt gtttttcata aaaaaccccc gctgagctaa gctcagcggg ggtttttcgt
     6301 acaaatagga aatatcaatt aatctttgca gaagcttctt tcgtatgcaa tgaccttcca
     6361 ggcttcttct tcgctgatgg tggctgggac taaaggcacc atgcctgtgc cagggctacc
     6421 gttcttgatg acccacatga gttcgccatc tttccgtttc ttgtgaaatt tgcaattggt
     6481 gaagtcacga ggccctgggg tcaagacggc tcctgccggc ccttggccat tgccttcttt
     6541 gccgtggcaa ttaatgcatg tcccttttcc ctcatacaag gctttgcctt ccgccacgat
     6601 ttcaggcgaa gcatcttcgg ccttggaata taagtctgtt ttgtcttttt tggctgcaga
     6661 gcgttgatca gccggaaccc gggaaggaat tggatctttc tctggtccgg caatggctaa
     6721 accagtggtc aaaaacatgg cggcggttaa agttccaagc actttcatcg aaaacgtcat
     6781 gtaagcctcc taaggttaaa cagagttgat gagaccgtaa aaaaacatgg ggatggcatt
     6841 atcgcttccc ctttttttcc tctttttgaa tttgtgttgt ttgagcagag tcttaaacta
     6901 aatcacattc aaaaggacac ccttaaccca ctcaaacttt ttcatcatat caatgagttt
     6961 tttagcctgt caagaagttg aaaaagagtt gatgttgtgt cctgtagaat ttttccaatg
     7021 tgggctaagt gggctgcttt cactggagat tttgagaagg gggtgtggaa ggggatgggc
     7081 aggcctaagg cttttcaagc tttgttcaaa taggtatggt gcatcataat ttatgaaaat
     7141 tttgaagggc gggaggatgg acgattgtgt ggggctatag tccgtcgtgc tgtgacgtca
     7201 gggcttcaaa tgaatgtccc ggaccacctg acctggcttt ccaaatggac cttgtaattc
     7261 tccattaagg cggacgatca caccggccgc attccctaac gtgagcaagt attgtttatc
     7321 cgccttccag gtgctttttt gtccaggttg taataaggct tcatgcgggg cctgatcgtc
     7381 cgactgcacc accacccagg tcagttgtgt ggcttcgatt tctaggacca gagttccatc
     7441 gatcccggtg gccttttcgg gaacagggga aacaggcaag gtcggtaccg gtcgaggttc
     7501 agggggtagc tgtgaaggta tgatatcagg tgggggagga gaagtgagct ggatctcggt
     7561 gtcgacgggg aattccgatg aaacgctgtc gggtaactct tcaatatggg gggtgggatc
     7621 gagtatttct ttattctgca ttccactgat tggttcagga gtttcggatt caggtgggag
     7681 tggaacggtt tcctgctgcc agggtaggag ataaaagagg actccaccga tggccagaaa
     7741 aagaatgaca acaaggttcc aattgaatcg cttgcgatgg gctgcttgca atgtgacctg
     7801 gatgtgttgc tgctcctctt gggtgcgatc atagaaatcg ctggaggtgg ttaggaatag
     7861 ctgaagggca ttatcttcat ccagacccaa cgaacgggca tacgaccgta cgaacccttt
     7921 gacgaatact ttggccggta ggcttgcgaa gtcctcttcc tcaagtgctt gaagatggcg
     7981 ctgttgaatg cgagttttgg aagcgatctg ttccaacgat aaacgttgtc gttcccgggt
     8041 ctgtcggaat aatccaccaa gagtttccat ggcgttatcc tgaatgagat aagcctttcc
     8101 tgtattcatt ggattgtgat gtgaaggcac acatcatgta aatggtgtgc ataccgaaag
     8161 tcttcaggtc tatgacaaat cttcaaaatg cgttaaatgt ttaaaggaag aaaatcgttt
     8221 atcaatgtct tcacgggtta gatgcctgag gcggtccaga ctgaattcct ccacattaaa
     8281 tgaggccatg acgcttccaa aaatgatggc ctgtcgaagc ccggcttgcg agtaattttc
     8341 cgtagcagaa aggtacccca tgaagccgcc ggcaaaggtg tcgccggcgc cggttggatc
     8401 tttgaccgct tccagtggat aggctggagc cccaaaaatt tcctgctgat gaaacatcaa
     8461 gaccccatat tctccgcgtt taataatgag ggttttgggt ccacgcgata agatccgctt
     8521 cgccacttgt acgaggtttg cgtcatcgcc caaagcacgg gcttccccgt cattaataac
     8581 gaggatatcg atatgttcga ggactttcca taacgcttcc cttttcccct caatccaaaa
     8641 gttcatggtg tcgcaggcca cgattgatgg ccgcttcact tgattcagga catccagttg
     8701 taattctgga tcaatgttac ccagaaacaa ggcggatggc gtggtatagc tggccggaat
     8761 tttcggccgg aacgtttcta gaacattgag gtgtgtttcc agggtttgag cttcgttgag
     8821 ttgataggaa tattctcctc gccagcgaa
//
LOCUS       DAORAQ010000111         8824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1263_length_8824_cov_60.122477, whole genome shotgun sequence.
ACCESSION   DAORAQ010000111 DAORAQ010000000
VERSION     DAORAQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1263_length_8824_cov_60.122477"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..971)
                     /locus_tag="PKM72_09930"
     CDS             complement(96..971)
                     /locus_tag="PKM72_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61149.1"
                     /translation="MNTDNQTTQCRRRRISTLAPGYQSSTKMTQSMILVYLVMIIVLL
                     DVLSGCNGPSPYRLQAHFASEPEGSQIIKGKISTHSQNRLPVGMAIVMHPDSSHGPLA
                     VTEDSWPQFAARVKREVQGHFPVSMYEVVRVDGIPSGEKVSLLEGMGGNSPIDMVLIV
                     LPTAIEIKSPAKFDLLPEVSMLTGYQTENHATVELGLLDLQSGKLLLQSQGNSYATLD
                     QLDVPLSSNRYPRVRGSAMTSTIYPEEGRAFETLRMVALEEALNQAVMKLTDEWHDSQ
                     GGLTLSESPGAGEAS"
     gene            complement(1947..3479)
                     /locus_tag="PKM72_09935"
     CDS             complement(1947..3479)
                     /locus_tag="PKM72_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNP61150.1"
                     /translation="MKPRSSVFSSRPHPHLYEINTWVWLHDLSQKMNRTIRLGDVPDQ
                     EWDELQAKGFDCLWLMGLWERSPLGRRIARQHSDLQQDYAKALPDWQEYEVVGSPYAI
                     RAYQPDPELGSWEQLKDVLRKLHDRGIKLILDFVPNHTALDHEWTTRHPEYYVQGTSR
                     DVTNFPSQFFPIDTSKGIRYLAHGKDPNSPAWTDTAQLDYFNPDTRKALIRELQHVAK
                     YCDGVRCDMAMLVLNEVFAQTWENHVKGDVCPSGEFWTEAISLLPDCMWIAEVYWNRE
                     WELQQLGFQFTYDKILYDRLRHSTPHEVALHLQADTIFQSKLVRFLENHDEPRSAVAF
                     GNVRLPAVGVLMATLPGMRLYHQGQLSGKRIRIPVQLCRAQDEPPDEATVAFYDRILA
                     ITNEDVFHAGKWAMLTVHSAGDDSFSKVVAYQWTTERVWKLIVVNLSPMVAQAHIRLN
                     GESEIEPGTFPDYTLYDQFNDLSFEGSRKDLIKSGLYVRLDPFECHIFSMEDGVIKSA
                     KR"
     gene            complement(3959..>4354)
                     /gene="rnk"
                     /locus_tag="PKM72_09940"
     CDS             complement(3959..>4354)
                     /gene="rnk"
                     /locus_tag="PKM72_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNP61151.1"
                     /translation="EFDLNRLTELLGVWQTFKGSKSTSIHLEGLSEELDRANIVDPKD
                     IPPDVVTMNSRVRLSDTSKDEDLIYTLVFPRDADAEAGKISILAPIGTAILGYKVGDI
                     IEWPVPLGIRKVKIEEVLYQPEAAGDFHL"
     assembly_gap    4355..4454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4677..7403)
                     /locus_tag="PKM72_09945"
     CDS             complement(4677..7403)
                     /locus_tag="PKM72_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNP61152.1"
                     /translation="MPVSDKKRSDMCWHALDGESVLKAVASQVGGLTQDVAAERLALY
                     GPNRLRPPKKQASWQRFLAQFHNILIYILLGAGVVTAILGHWVDTSVIFGVVVINAII
                     GFLQEGKAERAMDAIRRMLSVQASGLRDGARCSIPAEDLVPGDIVFLQSGDKVPADLR
                     LLKVKALRIEEAALTGESVPVEKHIQPVPETASIGDRKGMVYSGTLVTYGTGTGVVVA
                     TGDATEIGRISAMLAGVQTLTTPLLRKIGEFGSRLSLAIITGAVGVFFFGIFFREYGF
                     SDMFLASVGLAVAAIPEGLPAIMTITLAIGVQAMARRQAIIRRLPAVETLGSVTVICS
                     DKTGTLTRNEMTVQTLATAAYAVDVSGVGYDPHGGFSLFGNTVVLTDLPDVMELARAG
                     MLCNDADLAQVEGAWQVNGDPTEGALVTLGRKAGLDFRFEHEMWPRTDVIPFESQHRF
                     MSTLHHDHAGHGFMYIKGAPERVLEMCAFQRSLGEDHPLNSRSWQDRIEQMAGQGQRV
                     LAVAFASTNHAHRELRFQDVEQGLTMLGLIGIVDPPRAEAIEAVRQCQQAGIRVKMIT
                     GDHLVTARTIGLQMHIGDGKQAISGQTLETLSDEELAGVVRDIDIFARASPEHKLRLV
                     EALQAGGEVVAMTGDGVNDAPALKRADVGVAMGVKGTEVAKEAAEMVLADDNFASIAH
                     AVEEGRRVYDNIQKSILFILPTNVAEAGVIVAAILLGTMLPITPVQILWVNMITAVTL
                     ALALTLEPPESDVMRRPPRNPREAILSRFLLWRIGFVSVLAVTGTFGLFLWECDRGTS
                     IETARTIAVNMLVVFEAFYVLNARSFYGSVLSPKGLFQNPYVPLTIGMVLGIQGLFTY
                     TEVMQTLFHTTGIDGLAWFRIVGIGLVIFLLVEFEKYVFRNLLKLRTPDLKT"
     gene            complement(7519..8463)
                     /locus_tag="PKM72_09950"
     CDS             complement(7519..8463)
                     /locus_tag="PKM72_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61153.1"
                     /translation="MKVLVAVDPSEHTQEAIRFVKSVDWPKTSEMYLIHVIEMKHASS
                     LIPSDGPSSWDRVISEARGKLFTKAKGLLKHTQKEILEERAVTIKSLVMEGLPGAQIL
                     QAVEDYQIDLVILGTRGLSNVKRFLLGSTSDWVMREAPCSVLLVREKLSKVTMGKAAA
                     KILLATDGSSVALSTVDMLGLLACKTPPKVTVAHVVGKPVYLEGWFGRKGKPAFKQLA
                     GQFLEKSHKEAASHLEELSQRVKDLGMKVDTVLTKGDPAEEIVKIAERSKPRLIMVGS
                     KGFKGGKPVPLGDVVRKIARHAPCSVFLIRPGRSKQES"
     gene            complement(8507..>8824)
                     /locus_tag="PKM72_09955"
     CDS             complement(8507..>8824)
                     /locus_tag="PKM72_09955"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNP61154.1"
                     /translation="EPGKETTQSKEEIKEERVSVLRYLGTDGRDIHWDVIRLAMSSVA
                     QLAMVPFQDVLGLGSECRMNRPGTLKGNWEWRVHPGEMTENVGNRLRDLTGLFDRLPI
                     PMP"
BASE COUNT         2229 a         2409 c         1972 g         2114 t
ORIGIN      
        1 tcaaactagc tcctccgata tagaaaaccg gcttcaagcc acgggggcct ctccccatgg
       61 cttgctctca tcgttctttc catattttcg catcttcagg atgcctctcc agccccagga
      121 gactcactga gagttaatcc cccttggcta tcatgccact catccgttag cttcataaca
      181 gcctggttaa gagcctcttc caatgcaacc atcctaagcg tttcaaacgc cctaccctcc
      241 tccggataaa tcgtgctggt catcgcggat cctctcactc gtggataacg attcgatgac
      301 aatggaacgt ccagctgatc caacgtggca taagaattcc cctgtgactg tagtaacaat
      361 ttcccagatt gaagatctaa caaacctaat tccacagtgg cgtgattttc agtctgatat
      421 ccagtcagca tgcttacttc cggaagaaga tcaaacttag cagggctctt gatctctata
      481 gctgtgggta gaacaattaa aaccatatca atcggactat ttcctcccat tccttcaagg
      541 agagaaacct tttctccgga tggtatcccg tccacgcgaa caacctcata catagaaaca
      601 ggaaaatgcc cctggacttc ccgtttcact ctggccgcga actgaggcca cgagtcctca
      661 gtgaccgcga gtggaccgtg tgaggagtcg ggatgcatga cgatggccat gcctaccggt
      721 agtcggtttt gggagtgagt agagattttt cctttgatta tttgagagcc ttcaggttca
      781 cttgcaaaat gtgcctgcaa tcgatacgga gaggggccgt tacacccgct caacacgtcc
      841 aagaggacga tgatcattac caggtatacc aaaatcatcg actgcgtcat ttttgttgag
      901 gactgatagc ctggagcaag ggttgaaatg cgacgtcttc gacattgcgt ggtttgatta
      961 tcggtattca tggatagatc tcctttacaa ttggaattag gttcttacct gaagaatcaa
     1021 aggaaaagtg gtgctaaaat cccatctgcc gatcaagaca gaattgggag ggtggttgtt
     1081 aaaacctacc caccccacct ctcatcaatt tagcgagagt ccaatcaggt tcatcttcct
     1141 tatcctcagt cttgactaaa gtccgctcca catccatttc tccaacttca gtggcacaag
     1201 ccaaattcca gtcggtctca tccgccacac caataagcca tgtgaaccgg tgcttcagtc
     1261 ctccactacc taattagaac ttatggaaaa aattcctgcg accattacgg cgagcatcca
     1321 acgataagaa tatgttggtt ggtaaatcat gacatctcct cctttgtggg tgacgggatt
     1381 accgtccggg ttactctgat tgatggattt ggtgattgca tagaggtatt taatatggat
     1441 gaaagttaca tagacaaata cataatattt tattcattat taaaattttt agatgtatat
     1501 taaattcctg aatcttcaat catgagtcaa aatcatgttg gacgtcaggt gaaaccacat
     1561 ccgatcagaa tacattcggg aatgcatggg ttcggtagcc aatgaggcac atactcgatt
     1621 tcagactgaa ccctcttaat aaagcatggg gggtagggct ttgggatcag gccatctcca
     1681 cgccatcatg ccgggcaagt gtaatcgaca atccgtctta cggaagttgt gaggcgtaag
     1741 gaggtaagga gaattttggg atggctccca actgccaccc cgcgaggatg acgaaaaagg
     1801 aaaggccgtt gttactgacc aaggaaggga acctgacaaa cagagactgc ggggatagtg
     1861 agttactaaa ttttcgacta caaactttcg gaaggggccc acatcgattc atgtaaattc
     1921 aaacactctg aaccgtattt ctaactttac cttttagcag acttaattac cccatcttcc
     1981 atagaaaaaa tatggcactc gaaaggatcg agacggacgt acaatccgct tttgatgaga
     2041 tcctttcggc tcccctcaaa ggataaatcg ttgaactggt catacaaagt gtagtcgggg
     2101 aaggtgcccg gttcaatctc agactctccg ttcaggcgaa tatgcgcttg ggccaccatg
     2161 ggactcaggt tcaccacgat gagtttccaa acccgctcgg ttgtccattg atacgccacg
     2221 accttactga aagaatcgtc gccggccgaa tggacagtga gcatggccca tttcccggca
     2281 tgaaacacgt cttcattggt gatcgccaga attcggtcat aaaacgccac ggtcgcttca
     2341 tcagggggtt cgtcctgggc tcggcaaagt tgaaccggaa tccggattcg ttttccacta
     2401 agttggcctt gatgatacaa acgcatgccg ggcaatgtgg ccatcagaac cccgaccgca
     2461 gggagacgga cgttaccgaa cgccacagca cttctcggct catcatggtt ttccaaaaac
     2521 cgcactagtt tgctttgaaa gatcgtatca gcctgaagat gcagggcgac ctcgtgtggg
     2581 gtcgaatgtc gaagccgatc atacaagatt ttgtcgtagg taaactgaaa accaagttgt
     2641 tgaagctccc attcacgatt ccaatatacc tcggcaatcc acatacaatc cggcaggagg
     2701 gagatggctt ctgtccagaa ctccccggaa gggcagacat cgcccttcac atgattttcc
     2761 cacgtctgtg caaacacctc attgagcacc aacatggcca tatcacagcg aacgccatca
     2821 caatatttgg caacatgttg caattcgcga atcagcgcct ttcgcgtatc aggattgaag
     2881 tagtctaatt gtgcggtgtc cgtccaagca ggggaattgg ggtccttgcc atgtgccaga
     2941 tatcgaatgc cttttgaggt gtcgatggga aaaaattgcg aggggaagtt tgtgacatcc
     3001 ctggaagttc cttgaacata atactccggg tgtctggtgg tccactcatg atccaatgcg
     3061 gtatgattgg gcacaaaatc cagaatcaat ttgattccgc ggtcatgcag ttttcttaat
     3121 acgtccttca attgctccca ggaccccagc tcaggatcag gctgatacgc ccgaatagca
     3181 tagggggaac ccacgacctc gtattcctgc caatcgggaa gagctttggc atagtcctgt
     3241 tgcaaatcgg agtgttgtct agcgatccgc cggccaagag gactgcgctc ccacaagccc
     3301 atgagccaca ggcaatcaaa cccctttgcc tgaagctcat cccattcttg atccggaacg
     3361 tcaccaaggc gaattgttcg gttcattttc tgagagaggt cgtgcaacca cacccatgta
     3421 ttaatttcat aaagatgggg atgcgggcga gatgaaaaaa ccgaagatcg cggtttcata
     3481 aaggtgaatc ctatccgtct tactcaagcg tcctagggga ttccacacga tcaggaatgt
     3541 gtttgccggg agaggtgctt cctttcccaa aaggactatc ggggattcca tccctcgtca
     3601 cggccaatgt tattattggg gcatttacac aaaggataca atcgaataga tccacgatga
     3661 caacgggcgg ggttaacgga aaacccctat cctgccaaca atgataaacc ataaagcgtt
     3721 gctctcgacc aggccagctc acctgagttg ttattcttag acgacggcga aggatctgag
     3781 ccagggatga aaaatatggg acaggcatct ccatcatcgc cataccggac atttttcatt
     3841 gggcatccaa accgaattcc cttcaaatgg atgccggcct actgatggcc acatttgact
     3901 tctttgccgt aaaacgctcc catgcggttc atgttgaacc gatcgagccc ggagccggtt
     3961 aaaggtggaa atctccggcg gcttcaggtt gatagaggac ctcttcaatt ttcacctttc
     4021 gtattcctaa cggaaccggc cattcaataa tatcccccac cttgtacccg agaatggcag
     4081 ttccaattgg tgcaagaata gaaatctttc ctgcctcagc atcagcatca cgcggaaaaa
     4141 ccaacgtgta gatcagatct tcatctttgc tcgtatctga caatcggacg cgtgaattca
     4201 ttgtcacgac atcaggcgga atgtccttcg ggtcaacaat attcgcccgg tccaactcct
     4261 ctgataaccc ctccagatga atactggtac tcttgctgcc tttgaacgtt tgccagacgc
     4321 ctaataattc agtgagtcta ttcagatcga attcnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnatcttt gagcaacaac ccaccgttgg catgaggatt cggacccctt
     4501 cgccgatcac ccttagtttt caaacccgct agctttggaa tgcgtttgcc tgtcgcgtca
     4561 aatcattcct ctgatttgta gccattcatc ctcatatcgg gtagttgaat ttgatccttc
     4621 gagaggatcc ttcccggtcg ccatcttaac aaccgtaccg agtcaggacc catccgtcac
     4681 gttttcaggt cgggagttct cagtttcagc aaattccgaa acacatactt ctcgaactca
     4741 accagcaaaa aaatcaccag gccaatgcca acaattcgaa accacgccaa tccatcaatc
     4801 ccggtggtat gaaacaacgt ctgcatcact tcggtatagg taaatagccc ttgaatccct
     4861 aagaccatac ctatggtgag cggaacatag gggttttgaa acaatccttt gggggaaaga
     4921 accgagccat aaaaagaccg cgcattcaag acataaaagg cttcaaaaac caccaacatg
     4981 ttgacggcta tggttctcgc ggtttcaatt gaggtccccc ggtcgcactc ccagaggaac
     5041 aggccaaacg tgcccgttac ggctaagact gatacaaagc cgatcctcca caggagaaac
     5101 cgtgacaaaa tggcttcacg gggatttcga ggaggacgtc gcatgacatc cgattcaggg
     5161 ggttcgaggg tcaaggccaa ggccagggtg acggcggtga tcatgttgac ccacaaaatt
     5221 tgtaccggcg taatcggcaa catggtccct aataaaatgg cggccacaat cactccggct
     5281 tcggcgacat tggtgggtag aataaacaag atggattttt gaatattgtc ataaacccga
     5341 cggccttcct cgaccgcatg agcaatggag gcaaaattat catcggccag caccatttcc
     5401 gccgcttcct tggccacttc cgttcccttt actcccatcg ccactcccac atccgcccgt
     5461 ttcagcgccg gcgcgtcatt cacaccatct ccagtcatgg ccaccacttc cccacccgcc
     5521 tggagggctt ccaccaaccg caatttatgt tcgggacttg cccgggcaaa tatatcaata
     5581 tcccgcacaa cacccgccaa ttcctcatca ctcaacgttt ccagtgtctg tcccgaaatg
     5641 gcctgttttc catctccgat atgcatctgt aagccaatcg ttctcgccgt caccagatga
     5701 tccccggtaa tcattttgac ccgaatcccg gcttgctggc attgtcggac cgcttcaatg
     5761 gcttcagccc ggggtgggtc aacaataccg atgagcccca acatggtcag accctgctcc
     5821 acatcctgaa accgtaactc tcgatgtgca tgattggtag aggcaaaggc cacggccaac
     5881 actcgctgtc cctgcccggc catctgctca atccgatcct gccacgaccg gctattcaaa
     5941 ggatgatcct cccccagact ccgctggaac gcgcacatct ccaaaacccg ttcgggggca
     6001 cccttgatat acataaaccc atgcccggca tggtcgtgat gcaacgtcga cataaaccgg
     6061 tgttgcgatt caaagggaat cacatccgtc cgtggccaca tctcatgttc gaatcgaaaa
     6121 tccaatccgg cttttctccc gagggtaacc aacgccccct ccgtcggatc cccgttgacc
     6181 tgccaggccc cctccacctg agccagatcc gcatcgttac acaacattcc ggctcgagcg
     6241 agttccatca catctggaag atcagtcagc acgaccgtgt tgccaaacag agaaaatccg
     6301 ccatgaggat catatccgac gccactgaca tccacggcat acgctgcggt cgccaatgtt
     6361 tgtaccgtca tttcgtttcg tgtaagagtg ccggttttat ctgaacaaat aaccgtgacg
     6421 gaacctaagg tctccacagc cggcagccgg cgaataatcg cttggcgtcg ggccatcgct
     6481 tgaaccccga tagcgagcgt gatggtcata atggcgggaa gtccttcagg aatggccgca
     6541 acggcaagtc ccacgctcgc cagaaacatg tcactgaaac catactcccg gaaaaaaatt
     6601 ccgaagaaaa acacacccac cgctccggtg ataatggcca gacttaaccg gctcccgaat
     6661 tctccgatct ttctgagaag cggcgtggtt aaggtctgca ctcccgccag catggcgctg
     6721 atccgtccaa tttctgtggc atctccggtt gcgacgacca ctccggttcc tgttccatag
     6781 gtcaccagag ttcccgaata gaccatgcct tttcgatcac caatggacgc ggtttcggga
     6841 accggttgta tatgcttctc aaccggcaca gactcaccgg tcaaggcggc ttcttcaata
     6901 cgcaacgctt tcaccttgag aagacgcagg tctgccggaa ccttgtcccc tgattggaga
     6961 aatacaatat ccccaggcac aagatcttcc gctggaatgg agcaccgggc tccatcacga
     7021 aggccggagg cttgcacgga caacatccgg cgaatggcat ccatcgccct ttcggctttg
     7081 ccttcctgaa gaaatccgat gattgcatta atcaccacga cgccaaaaat cacgctggta
     7141 tccacccaat gaccgagaat ggcggttaca acacctgctc ccaggagaat ataaatgaga
     7201 atattgtgaa actgggcgag aaaccgctgc caggaggctt gttttttagg tgggcgtaaa
     7261 cgattggggc catagagggc cagacgttcg gcagccacat cctgagttaa ccccccaacc
     7321 tgactggcca cagccttgag aacggattca ccatccaagg catgccagca catgtctgac
     7381 ctttttttat cggatacagg catcgtgctc atttcaattg aagttaagag aacgcatgag
     7441 aagcaggctc agaattctct gcctccctat ggaaacaccc tgatggtctt gagtgtttct
     7501 ggagaaaggg agcggccatt aactctcttg cttagatctg ccagggcgga tgagaaacac
     7561 cgaacatggg gcatggcggg cgatttttct gaccacatcc cccaatggga cgggcttccc
     7621 ccccttaaat ccttttgatc ccaccatgat caatctgggt tttgaacgct cggcgatttt
     7681 gacaatttct tcagcagggt ctcctttagt caagacagta tcgactttca tgccgaggtc
     7741 tttgactctt tggctcaact cttccagatg gctggccgct tctttgtgag atttctccaa
     7801 gaattgtccc gctaattgtt taaaggcggg ttttcctttt ctcccaaacc aaccttccaa
     7861 atacacaggc tttcccacca catgcgccac cgtcaccttt ggaggggtct tacaagccaa
     7921 cagaccgagc atatccacag tactgagtgc aacggatgat ccatcggtgg ccagaagaat
     7981 tttggcggcg gcttttccca tcgtcacctt actgagtttt tcacgaacca ataacactga
     8041 acacggggct tccctcatca cccaatcact ggtactccct agaagaaacc gtttcacatt
     8101 ggaaaggcca cgggttccta agatgaccag gtcaatttga tagtcctcca ccgcctgtaa
     8161 aatttgcgca cccggaagcc cttccatcac cagggactta atggtcacag ccctttcttc
     8221 aagaatctcc ttctgcgtgt gcttaagaag acccttcgct ttggtgaaca gcttccccct
     8281 tgcttcggaa atgacccgat cccaactgga aggcccatcc gacggtatca gagaagacgc
     8341 atgtttcatc tcgatcacat gaatgagata catttctgat gtctttggcc aatccaccga
     8401 tttcacaaat cgaatggcct cctgcgtatg ttcggaagga tcaaccgcca ctaaaacttt
     8461 cattgtcatc tccctttccg gcaaaggctt ttatttttta ggatttctaa ggcatgggta
     8521 ttggcagccg atcaaataaa cctgtcaggt cacggagtcg gttcccaaca ttctctgtca
     8581 tctcaccagg gtgaacccgc cattcccagt tccccttgag cgtgccgggg cgattcatcc
     8641 ggcattcact ccccaatccc aaaacgtcct gaaacggaac catggcgagt tgggcaacag
     8701 aactcatggc cagtcgaatc acatcccaat gaatatctcg accgtcagtc ccgagatagc
     8761 gcaacacact cacacgctcc tccttgatct cctctttgga ttgggtggtt tccttccccg
     8821 gttc
//
LOCUS       DAORAQ010000112         8774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1272_length_8774_cov_67.112169, whole genome shotgun sequence.
ACCESSION   DAORAQ010000112 DAORAQ010000000
VERSION     DAORAQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_8774_cov_67.112169"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKM72_09960"
     CDS             <1..524
                     /locus_tag="PKM72_09960"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNP61155.1"
                     /translation="EVLKNFFLLTTQPVLYVGNTDENPDPAILADFQAFAKERGSESV
                     VICGKLESEIAQLSGEDREIFMKDLGMEQSGLEKVVVAAYKTLGLCSFLTSGVQEVRA
                     WTIPIGAKAPQAAGVIHTDFEKGFIKADIYAFADIDTHKSEATLREKGLIRSEGKEYV
                     VKDGDVCHFKFNV"
     gene            770..1126
                     /locus_tag="PKM72_09965"
     CDS             770..1126
                     /locus_tag="PKM72_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNP61156.1"
                     /translation="MKGIHTFSLLGASALLLTSLGLAIGDDGREKVAVANLPTSVTME
                     EAIKTATTQFPGKVIEAELESEDGKVIYEVEIVNAAGEIREIEIDAQSGKILSSELED
                     QEAHEKGASQKDCGEA"
     gene            1341..1589
                     /locus_tag="PKM72_09970"
     CDS             1341..1589
                     /locus_tag="PKM72_09970"
                     /inference="COORDINATES: protein motif:HMM:NF021505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61157.1"
                     /translation="MGFLGGVAAAITGTWAEEAIKRTGIAKSMIETHEALAFVSSGIF
                     GVLLWGGLVLRDKFRRKTSFSYFLIAAIGLGTLSAKGR"
     gene            1693..2370
                     /locus_tag="PKM72_09975"
     CDS             1693..2370
                     /locus_tag="PKM72_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP61158.1"
                     /translation="MRILLVEDDSSVSGFIVKGLREEQYAVDLATDGEMGLAMAMTSS
                     YDLIILDIMLPKMNGIDLCRRLRAKRQTTPILLLTAREAVEDRVTGLDTGADDYLTKP
                     FAFAELLARLRALLRRGSAQPTPRLTIADLELDPISHRISRAGQTIVLTNKEYSLLEY
                     LMRNTGRVLTRTAITEHVWDIHYESVTNIVDVHIKTLRSKMDRDFSPQLIHTVRGIGY
                     VLKIPDA"
     gene            2374..3819
                     /locus_tag="PKM72_09980"
     CDS             2374..3819
                     /locus_tag="PKM72_09980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HNP61159.1"
                     /translation="MQAFRTRIRLVALGFIVILLGLFSLLLYLGFSTILHGHIDEELV
                     ALAEEESYRVDLATDHVEVSSHRQDEGNKEDEEDEREEFDDEDELQQAIRYSAILDPT
                     GRILWSGQSTENRIPVASQVIAQALKGETVFETFHEPGRSPIRRISIPLAIEDHGRFI
                     LQTEKSLQFVEETLQWLAWLLCGASAISLLFAWWGSGRLAGEALAPVKALSQTAETIS
                     GQTLSTRLTLTSPYAEFQRLAHTFNGMLDRLQQVFEGQRRFVADAAHELKTPLTAMKG
                     NFEVSLQRARSGEEYRETILSNLAEVDRLTAMTKSLLTLAQFAGDRPPLALQTLDLPP
                     LVEDVVSELSVLAHEGGIHLQTEFEPVPPFLGDASQLKQALINLLDNALRHTPSDGTI
                     TVRVRFHEGSIRLSVEDSGPGIDSQHLPHLFERFYRIDQARDRHSGGTGLGLAIVKEI
                     VEAHGGTIHAQSQVGYGTTFTITFPTGKNHR"
     gene            3877..5079
                     /locus_tag="PKM72_09985"
     CDS             3877..5079
                     /locus_tag="PKM72_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP61160.1"
                     /translation="MKKLNHRYWRWLPVFCLLGLFPLGCSDTQPPAGSPPDKDLAVSS
                     TDFSPHSEQTFHVDRVPDQARQRIRIEEVKERLTPHSISAPGHVALDLSHVAKVSSRI
                     PGQVDKVMVQLGDRVNKDQPLAAVESMRLDELVQEYLVAKSKLDVASSNFRRTKQLLA
                     ENIISQRRFLEDRGQQIEAQAVYQHVREKLLNMGLSPQELQQMEHGSHLEGHKYLLRA
                     PLSGTVVNQKIVLGQGVAAGDEFFEIVDTSQVWVFANLPIEHARRFKEGDQGEVLPRG
                     GEPITARLTYIAPVADATTRTIRVRFDVENSNGRLKPNEYVDVRLIDQQQPVLSIPLS
                     ALTMVEGVRGVFVQRGNGYDFVFIETGQEGGGLVEIKRGLKLGEHVVTEGVFDLKNAI
                     MKPSAHEH"
     gene            5215..8331
                     /locus_tag="PKM72_09990"
     CDS             5215..8331
                     /locus_tag="PKM72_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNP61161.1"
                     /translation="MERLLTLSLQYRFFTVVALLLVSASGMWSLTNLTIDAVPDLTPV
                     QVQILTRSPALGPIEVEQFITFPIETALSGIPGLQEVRSVSRYGLSAVTAIFEEHLDL
                     YFVRQLVSERLALATERIPAAYGRPMMGPLSTGLGEVYQFTLTGQGHSPMFLRTLLEW
                     DIGRRLRAVPGVVEVNIWGGETKQFQVVVDPSKLLAFNLTLKAVFDALAQNNAVAGGG
                     YIEHERELYLIKGEAMASTVTDLGQIVVSHGPGGVPIFIRDLGEVKEGAALRIGAATR
                     MGEGETVIGMVQMLAGANAQQVVEQVKKRVKEIQSTLPPGVLIEPYYDRTLFVSKVIN
                     TVKNNLLEGGLLVIAILFSFLGDVRAGFIVALAIPLSMLMAFTGMYHAGISGNLMSLG
                     AIDFGLLVDGSVVMIDNILRKLGKVTSQSKEDKLATIQSAAREVLRPIVFAVGIIIVV
                     YLPILSLVGLEGKMFRPMAMTVIFALAASLLFAVGGVPVLAYWFARARPNHEETWLIR
                     HARGLYQPWLDLSINRPAWVVIPGIVLFTLSLVIGSRLGIEFVPRLEEGDMAIQVWRL
                     PSVSMTESVQTALEVERALRKFPEVVQVVTRTGSPEVATDVMGIELSDVFVVLKPQDE
                     WTTATSKEALIDTFKTAVHEAVPGVGMSFTQPIEMRFNELIAGTRSDLAIKIFGEDLE
                     VLRHQGDLVARSLEQVPGAADVKAEQVTGVPRIRVIVDRDQIGRYGLNAKDLLNIIES
                     ARVGQTVGTVFQGQRRFDLIVRLSDNASAGPVALGNLLIPTPHGELVPLSRVATIKVD
                     EGPAQVSRQDIQRRLVVEANIRGRDLGSFVTEAQQIIRDRVSLPPGYYLEWGGQFKHL
                     EEASRRLAVVVPITLLLIIAILSVIFGTIRPAFLIFLNVPLALSGGIFALWIRGLPLS
                     ISAVIGCVALFGIAVLNGVVLVSRIQRLETEGLPTRDAVAQGAMDRLRPVLMTALVAS
                     LGFLPMAVATSMGAEVQRPLATVVIGGLITSTALTLFIIPALYGTVCRSNKQPAIPNQ
                     SHTSTIST"
     gene            8366..>8774
                     /locus_tag="PKM72_09995"
     CDS             8366..>8774
                     /locus_tag="PKM72_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP61162.1"
                     /translation="MWFKQIICLIMLYGLCWQPALAATDTTPQAAAPTYSLKEILNMA
                     LEKNPLMNESEGVIEQREGDKISASAYPNPTLWVQSGRGTVREPTGPTLTERYATLYQ
                     PIEWPGKRAARQQAAQAGLDGALASKEEAKLNLI"
BASE COUNT         2032 a         2340 c         2327 g         2075 t
ORIGIN      
        1 cggaagttct caagaatttt ttcctgctca ccacacagcc cgtgctctat gtcgggaata
       61 ccgatgagaa tccggatccg gcgatccttg cggactttca ggcattcgcc aaggagcgcg
      121 gatcggaatc agtcgtgatc tgtggcaaac tcgaaagtga aattgctcaa ctatccggtg
      181 aggaccgcga aattttcatg aaagatttgg gcatggagca gagcggcttg gagaaggtcg
      241 tcgtcgccgc ttataaaaca ctcgggctct gctcgttcct cacctccgga gtgcaggagg
      301 tcagagcctg gaccattccc atcggagcca aggcgccaca ggccgccggc gtcatccaca
      361 ccgacttcga gaagggcttt atcaaggccg acatttacgc cttcgcggac atcgacaccc
      421 acaagtcgga agccaccctg cgcgagaagg gcctgatccg ctccgaagga aaagaatacg
      481 tcgtcaaaga tggcgacgtc tgccacttca agttcaacgt ctagaccggt ttttgcttgc
      541 cggcatgctg gcgggcacgt taggattcaa ccgacaatgc gtttcccctt ccaatagctg
      601 cctttccgcc tttccaggtg ggtcgacaag ggcctatcct ggctcatgtt attctctcta
      661 aatgaagaat ttttcatgtc cgcttcatag ctgcttcatg tagatggtct attctgaatt
      721 tatgaacagc cggttgattc catcaatttc ttaagaagga ggatgtatca tgaagggaat
      781 ccacacattc agtctattgg gcgcctcagc tctcttgttg acttcccttg gattggccat
      841 aggtgatgac ggccgggaga aggtcgccgt ggccaatctg cccacttcgg tcaccatgga
      901 agaagccatt aaaaccgcga cgactcaatt tccaggaaag gtgattgaag ccgaactgga
      961 aagtgaagac ggaaaagtga tctatgaagt cgagattgtc aacgctgccg gagaaatcag
     1021 ggagattgag attgacgccc aatccggaaa aatcctgagt tctgaactcg aggaccagga
     1081 agcgcacgag aagggcgcct ctcagaagga ttgcggagaa gcctaataca tcgtcaaggc
     1141 cccatgcgta agaaagagta tggggccttt tctcctttcc ccacactgac gacacacagg
     1201 aggaaccatc ttgagctgcg ggtacttccc atgttcgtgc atttcctcat ccccttgcaa
     1261 ttcaccactg tgctatttga cgacgcagga gcatagttca aacggaacaa tttcggtaat
     1321 ggcgattttg acttctcatc gtggggtttc tcggaggggt tgcggccgcg attaccggca
     1381 cctgggcaga ggaggctatt aaaaggaccg gaattgccaa atcgatgatt gagacccacg
     1441 aggccctcgc ttttgtgagc tcaggaattt tcggagtcct cctatggggt gggctggtgc
     1501 tgcgagacaa atttaggcgg aagacctcgt tttcgtattt tttgattgcg gcgattggat
     1561 taggcacact aagcgccaaa ggtcgctagg ggggagattt ggtctacgag caggggctgc
     1621 ccttgtaaag gacgcaggac tccatgcgaa gcacctccgg ccttcacggt tatacttttc
     1681 agcgggagac agatgcgtat tctcttagtg gaagatgatt caagtgtgtc cggatttatt
     1741 gtgaaagggt tgcgcgagga gcaatatgct gtggatcttg ccaccgacgg tgagatggga
     1801 ttagccatgg ccatgacctc gtcctatgac ctgatcattc tggatatcat gctcccaaaa
     1861 atgaatggca ttgacctctg ccgccggctt cgagcgaaac gccagaccac gcccattctt
     1921 ctgttgacgg cacgggaggc cgttgaagat cgcgtgaccg gtttggatac cggggcagat
     1981 gattatttaa ccaaaccgtt tgcctttgct gaattactgg cacgtctcag agcacttctc
     2041 cgtcgtggca gtgcccagcc caccccccgc ctcacgatcg cagacctgga gctggatccg
     2101 atttctcacc gtatttcacg ggcaggccag accatcgtcc tgaccaacaa agaatattct
     2161 cttctggaat atcttatgag aaatacggga cgggtattga cacggacagc cattaccgaa
     2221 catgtgtggg acattcacta tgagtccgtc acaaacattg tcgatgtcca cattaaaacc
     2281 ctacgtagca aaatggaccg ggatttttct ccacagctga tccatactgt gcgggggatc
     2341 ggctacgtct taaagatccc ggatgcctaa cccatgcagg cctttcgcac tcgcattcgt
     2401 ctcgtcgcct taggcttcat cgtcatcctc ctaggcctct ttagcctgct cctctacctt
     2461 ggattttcga ccattttgca cggtcatatc gatgaagaat tggtcgcact cgccgaagag
     2521 gaaagttatc gggtggacct ggccaccgac catgtggaag tgtcctctca ccggcaagat
     2581 gaggggaaca aagaggatga agaagacgaa cgagaagaat ttgatgacga agatgaatta
     2641 cagcaggcca tccgttacag cgccatcctg gatcccaccg gccgtattct ctggagtggg
     2701 cagagcaccg agaaccggat tccagtggcg tctcaggtca tcgctcaggc tcttaaaggg
     2761 gaaacggtct tcgaaacctt tcacgaacca ggccggtccc ccattcgccg catctcgatt
     2821 cctcttgcca tcgaggacca cggacgattc attttacaaa cggaaaaatc tttacaattt
     2881 gtcgaggaaa ctcttcagtg gttggcatgg cttctctgtg gggcctccgc catctcgctc
     2941 ttgtttgcct ggtggggaag cggacgtttg gccggtgaag ccctggcacc ggtcaaggcc
     3001 ttgagtcaga cagcggaaac catttccgga cagacactct ccacccgtct cacactcacc
     3061 tccccctacg cggaatttca acgcctcgcc cacaccttta atggcatgtt ggaccgcttg
     3121 cagcaggtct tcgaaggaca acgtcgcttc gtggccgatg cggcccatga actgaaaacc
     3181 ccgttaacgg caatgaaagg caattttgag gtcagcttgc agcgggcacg gtcgggagag
     3241 gaataccggg agactattct ctccaacctg gctgaagtcg atcgactcac cgccatgacc
     3301 aaatccctcc ttactctcgc acaattcgcc ggcgaccgcc caccactcgc gctgcaaact
     3361 cttgacctcc cgcctcttgt cgaagacgtc gtgagcgagt tgtccgtgtt agctcacgaa
     3421 ggcggtatcc atctgcagac ggagtttgaa cctgttcctc ctttccttgg agatgcctca
     3481 cagctcaaac aagccctgat caatctcctg gataatgcgc tacggcatac cccttcagac
     3541 ggaaccataa ccgttcgagt ccggtttcac gaggggtcaa tccgtctatc cgtagaggac
     3601 tccgggccgg gaattgattc ccaacatctc cctcacctgt ttgagcgatt ctatcgaatc
     3661 gaccaggcac gggatcgcca ttcaggagga acaggcctgg gattggcaat cgtcaaagag
     3721 attgtcgaag cccacggagg aacgatccat gcacagagtc aagtcggata cggcaccaca
     3781 ttcacaatca ccttcccaac cggaaagaac caccgttaac atgaacgccc tgatagtctg
     3841 ttgtcagagg agattttttg tccgaatgag tcattcatga agaaactgaa ccacaggtat
     3901 tggagatggc tccctgtttt ttgccttctc gggctgttcc ctttgggttg ttccgacacc
     3961 cagccaccag cgggtagccc tccggacaaa gatctggctg tttcctctac cgattttagc
     4021 ccccattccg aacaaacctt tcatgtggat cgtgtccctg accaagcgcg tcaacgaatc
     4081 cgaattgaag aagttaaaga acgcctgacc ccgcattcaa tttcggctcc gggacatgtc
     4141 gcccttgatc tctcccacgt ggcaaaagtt tcctctcgca tccccgggca agtggataag
     4201 gttatggtgc aattagggga tcgggtgaat aaagatcagc cgctcgcggc cgttgaaagc
     4261 atgcgcttgg atgaattagt ccaggaatac ctggtggcca aatccaaatt ggatgtggcc
     4321 tccagcaact ttaggcgaac caaacaactc ctggctgaaa acattatttc tcaacgtcgg
     4381 tttctggaag accgtggaca gcagattgaa gcccaggccg tctatcaaca cgtccgggaa
     4441 aagctcctga acatggggct cagcccacag gaactccaac aaatggagca tgggagccat
     4501 ttggagggcc acaaatattt gctccgggcc cctcttagtg gaaccgtcgt gaatcaaaaa
     4561 atcgtgctgg gtcagggcgt agcggcagga gacgagttct ttgaaattgt ggataccagt
     4621 caggtgtggg tgtttgccaa tctgccaatc gagcatgccc gtcgtttcaa ggaaggcgat
     4681 caaggcgagg ttcttccccg tggtggagag cccatcactg cccggctcac ctatattgcc
     4741 ccggtcgcag atgccaccac ccgcaccatt cgcgtgcgat tcgacgttga gaattctaat
     4801 ggccggctta agcccaatga atatgtggac gtgcggttga tcgatcaaca acagcccgtc
     4861 ttatccattc ctctatcggc cttgacgatg gtagagggcg tgcgcggagt gttcgttcaa
     4921 cggggcaacg gatacgattt cgtgtttatt gaaaccggcc aggaaggcgg gggacttgtg
     4981 gaaatcaaac ggggcttaaa actgggagaa cacgtggtca ccgaaggtgt cttcgatctg
     5041 aaaaacgcca tcatgaaacc atccgcccat gaacattaac caccatcacc gtgggtgaga
     5101 acgtggaatt tgcgcacaaa tatgcgtgtt atttatcaag gcatgcctga cgaatcaggt
     5161 tctcttcttt cgtgttcccc gcaattagtc aaatgatgaa aaccctataa ctccatggaa
     5221 cgtcttctca cactctcact tcaatatcga ttttttactg tcgtcgcctt gcttttggtc
     5281 agcgcctccg gaatgtggtc gctgaccaat ctcaccatcg atgcggttcc cgatctcacc
     5341 cctgtccagg tgcagatcct cacccgttca cccgcgctcg gtccgattga ggtggaacaa
     5401 ttcatcacgt ttccgattga aaccgcactc agtggcatac cgggattaca ggaagtgcgt
     5461 tcggtctcac gttatggctt atcggccgtg acggcgatct ttgaggaaca cctggatctc
     5521 tattttgtcc gacaacttgt tagcgaacgt ctggcgttgg cgacagagcg cattcccgcc
     5581 gcttatggac gaccgatgat gggcccctta tcaaccggcc tcggagaagt ctaccaattc
     5641 acgctcacag ggcaggggca cagtcccatg ttcctgagga cacttctgga atgggatatc
     5701 ggcaggcggc ttcgtgccgt tcccggtgtg gttgaagtga atatttgggg cggagaaacc
     5761 aagcaatttc aggtggtggt cgaccccagt aagctcctcg ccttcaatct caccttaaaa
     5821 gccgtgtttg atgccctcgc gcagaacaat gcggttgccg gaggcggata cattgaacat
     5881 gagcgggaac tgtatctcat caaaggagaa gccatggcct cgacggttac cgatttaggg
     5941 caaattgtcg tgagtcatgg gccgggtggc gttcccattt tcattagaga tcttggggaa
     6001 gtcaaagaag gtgcggcctt gcggataggg gcggccacgc ggatgggtga gggggaaacg
     6061 gttatcggaa tggttcagat gctggcaggg gccaatgctc agcaggtcgt cgaacaggtg
     6121 aagaaacggg tcaaggagat tcaatcgacg cttccaccgg gtgtcctgat tgaaccctat
     6181 tatgaccgga ccctttttgt ttcaaaagtc atcaacaccg tcaaaaataa tttactcgaa
     6241 ggaggtctgc tggtcatcgc gattctcttt tcatttctag gagacgttcg ggccggattt
     6301 attgtggcct tagccatacc tctttccatg ctgatggctt ttaccggcat gtatcacgct
     6361 ggcatctccg gaaatctgat gagcctgggt gcgattgact tcggactgct ggtggatgga
     6421 tcggtcgtca tgatcgataa tattctgcga aagctcggga aggtcaccag ccagtcgaag
     6481 gaagacaaac tggcaacgat tcagagtgcg gcacgggaag tgttaagacc cattgtgttt
     6541 gcagtcggca ttattatcgt cgtgtatctt cccattttgt ccttggttgg gttggaaggc
     6601 aaaatgtttc ggcccatggc tatgactgtc atttttgccc tagcggcttc actcctcttt
     6661 gcggtaggag gcgtgccggt actggcctat tggttcgcac gcgcccgacc gaatcatgaa
     6721 gaaacctggc tcattcggca tgcccgaggc ctttatcaac cctggttaga cctgtccata
     6781 aaccggccgg catgggtcgt gattccaggc atcgtgctgt ttaccctcag tctggtgatt
     6841 ggatcgcgct tagggatcga atttgttcct cgattagagg aaggggatat ggctattcaa
     6901 gtatggcgcc tccccagcgt ctccatgact gaatcagtgc aaacggcctt ggaggtagaa
     6961 cgggcattga ggaaatttcc cgaagtcgtc caggtggtca cgaggactgg aagtccggaa
     7021 gtggccacgg acgtgatggg cattgaacta tctgatgtct ttgttgtcct taagccgcaa
     7081 gacgaatgga ccacggccac ctccaaagag gcattgatcg acacattcaa aacggctgtt
     7141 catgaagcgg ttcccggtgt tggcatgagc tttacgcaac cgattgagat gcgattcaat
     7201 gaactcattg ccggcacccg ttccgacctc gcaatcaaaa ttttcgggga ggatcttgag
     7261 gtcttacgcc atcaaggaga tttggtggca cgttccctcg aacaggtgcc cggcgctgcc
     7321 gatgtgaaag cggaacaagt gaccggggtc ccaaggattc gggtgatcgt ggaccgtgat
     7381 caaatcgggc gatacgggtt gaacgccaaa gacctgttaa acatcattga atctgcacga
     7441 gtgggccaga cggttggaac cgtctttcag ggacaacgcc gctttgatct gatcgtccgc
     7501 ctctcggaca atgcatcggc cggtccggtg gcgctcggca atctcctgat ccccaccccg
     7561 catggggaac tggttccgtt gtcccgcgtg gccaccatca aagtggatga aggacctgcg
     7621 caagttagtc gacaggatat tcaacgccgg ctggtagtcg aagccaatat ccgtggccgt
     7681 gatctgggtt cctttgtcac cgaagcccaa caaatcattc gcgaccgagt ttccctccct
     7741 cccggatact accttgaatg gggcggacaa tttaaacatc tcgaagaagc gtcacgccga
     7801 ctggccgtcg tggtgccgat taccttactg cttatcatcg ccattctttc ggtgattttt
     7861 ggaacaattc gtccggcctt cctcatattt ttgaatgtac ccctcgctct gtcgggcggt
     7921 atttttgccc tttggattcg aggccttccg ttgagtatct cggcggtcat cggttgtgtt
     7981 gcccttttcg gaattgcggt cttaaacggt gtcgttttgg taagccgtat ccaaagactt
     8041 gaaaccgaag ggcttccgac ccgggacgcg gtggcacagg gagccatgga tcgtcttcgc
     8101 cctgtcttga tgaccgccct cgtcgcaagt ttgggttttt taccgatggc cgtcgctacc
     8161 agtatgggag cggaggtcca acggcctctg gccacagtcg tgattggcgg tttgatcacg
     8221 tccacggcct tgaccctatt tattattcca gcgttgtacg gcaccgtctg ccgttccaac
     8281 aaacaaccag ccatacccaa tcagtctcac accagcacca tctcaacctg agaaggatgg
     8341 tgttgtcaca agaagggaac ttgtcatgtg gtttaaacag atcatttgcc tcatcatgct
     8401 atacggtctc tgctggcaac ccgcgttggc agcgactgac accacaccac aggctgcggc
     8461 cccgacctat agtctgaaag aaattctgaa catggcctta gagaaaaatc cccttatgaa
     8521 cgaaagtgag ggggtcatcg aacaaagaga aggagacaaa atctctgcct ccgcttatcc
     8581 caaccccacc ctatgggttc agagtggacg tggaaccgtc agagaaccta ccggtccgac
     8641 tctcaccgaa cgatatgcca ccctctatca gcccatcgaa tggccgggga aacgggcggc
     8701 acgtcaacag gcagctcaag caggattgga cggagccctg gcctcaaaag aagaagccaa
     8761 actgaatttg attg
//
LOCUS       DAORAQ010000113         8633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1298_length_8633_cov_65.982397, whole genome shotgun sequence.
ACCESSION   DAORAQ010000113 DAORAQ010000000
VERSION     DAORAQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_8633_cov_65.982397"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..518
                     /locus_tag="PKM72_10000"
     CDS             303..518
                     /locus_tag="PKM72_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61163.1"
                     /translation="MLGESRWLGHQMRVLMMSGGSDERVLRKLFREGAQGCILKPFHL
                     TSRKQVCREIITKMEIEEQVPHYFHVA"
     gene            589..750
                     /locus_tag="PKM72_10005"
     CDS             589..750
                     /locus_tag="PKM72_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61164.1"
                     /translation="MKSVEVDGIKKNGKSVICAHQRMVDNHYNEEGRRIGNLVCRECG
                     EVIHDPVEA"
     gene            869..1045
                     /locus_tag="PKM72_10010"
     CDS             869..1045
                     /locus_tag="PKM72_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61165.1"
                     /translation="MKAQSYSIMLGFDGSDVPTFEPHLSVQEINCADSARISDCLEGR
                     YTFKGLNPAWVPHN"
     gene            1408..1764
                     /locus_tag="PKM72_10015"
     CDS             1408..1764
                     /locus_tag="PKM72_10015"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNP61166.1"
                     /translation="MYRILDESAEGSVGIRVEGKLSTEEYDLLNTYLETLMQEVGPIN
                     FLCDMASFESLSGQALWEEMTDHLRYIHEFQRIALVGNRRMLKGEPKISVPWPKVQLK
                     FFTPDQTDEAWHWVKG"
     gene            2096..3076
                     /locus_tag="PKM72_10020"
     CDS             2096..3076
                     /locus_tag="PKM72_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNP61167.1"
                     /translation="MLSTQVNHLWSVVLAGGEGERTRPFIEQWLGGHKPKQYCTFVGK
                     RSMLQHTLDRADRLVNPEHKVTVIGRNHREFLNEAMEREKSGHVIVQPHNRGTAAGVF
                     LPLTFVRAWDADATVAIFPSDHFVFPEARFVETVRRGIRASHFLQDRLILFGVSPSHP
                     ELDYGWINLSEVLGWSSGSCIRLAGSFLEKPDIIEGKQAMANGALWNTLVMVGKVATL
                     WKLGWQCLPVMMERFEQLGSMIGTVHENETLHEIYRDMPVLDFSRELLQRIPEHLGVM
                     EFEEVLWSDWGQPERIVQTLKTLGKEPAFPSDILQRSLAPIQTIPRVGVA"
     gene            3076..3381
                     /locus_tag="PKM72_10025"
     CDS             3076..3381
                     /locus_tag="PKM72_10025"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNP61168.1"
                     /translation="MAKKRSVQPSDETPRQSPSAAKRKSVSRKKTSMREDPLEVQESM
                     IPGDTDSMNSQPTNGNVHARIARRAYELFHSRGGHHGQDLDDWLVAEQEIEAKDRSS"
     assembly_gap    4023..4122
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4123..4724)
                     /locus_tag="PKM72_10030"
     CDS             complement(<4123..4724)
                     /locus_tag="PKM72_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HNP61169.1"
                     /translation="MLDDTEYVKIFVALLAVINPLGALSIFIALTPGKEKAARKKIAK
                     IAAFSATLILLMALIAGEWMLEIFGISVSSFRVGGGILILLMAISMLQAELSPTVQTK
                     EEAEESNKKHDIAVVPLATPLLAGPGGISTVVVEAHKGTNLGHELLLGLIIIIIGLCL
                     WMALHLAPLISQRLGVTGINIFTRIMGLILAAIAVEIIANG"
     gene            5201..6142
                     /locus_tag="PKM72_10035"
     CDS             5201..6142
                     /locus_tag="PKM72_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61170.1"
                     /translation="MMKVLLAVEQTRISVGAIRILTRLSLPVGSNLFLLHVHPVPQKI
                     MGLAKERILKISQQVQEVQGEVHERARRFLGKVQNTLRRQDVVFHSLVKHGFPGEEIL
                     KTIRTKGIDLVVLGTRSYSKAAGFFLGSVSQWVLQEAPCSVLIARNTPHRSLNDGGMK
                     ILLATDGSSDSRAAVDFVKEMGLPDSSHMMILHVIKKHMYETERALTTGRENEKEFAK
                     LAEEVLEIQEQQGMKLLEQTSAVLSSPDLTTQKRLVYGNPATEILKTARMMKADLIVM
                     GSQGSTGVKRMILGSVSNKVVRSAGSSVAVIRSKRKK"
     gene            6338..>8633
                     /locus_tag="PKM72_10040"
     CDS             6338..>8633
                     /locus_tag="PKM72_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNP61171.1"
                     /translation="MSIRNFKYLFKPRSIAVIGASNRPHRVGRVVMQNLLKGGFEGPL
                     LPVNPKYQAVCGVLAYPDVQALPISPDLAILCTPPSTIPRLIQELGERGVRAAVILTA
                     GMRPKPSEKESDTEWTIMLETARNFGMRLLGPNTIGLLIPGLHLNASFAHMEALPGSL
                     AFVSQSGALCTAVLDWARSQGIGFSHFLSLGDSADVDMGDVLDYLGSNPDTRAILLYI
                     ESITQARKFMSAARAAARNKPVLAIKAGRHPEGATAASSHTGALIGRDEVYDAAFCRA
                     GILRVFEIDELFDAVETLERAGPLRGERLAIVTNGGGPGVLAADAFAEGGGSLARLSE
                     ATIHELTTLLPASWSHGNPVDIVGDARAERYAQALTSILKAPDVDAVIVAHAPTAVIT
                     SEEAALAVVNAVRASSSKKNVFTSWLGGQTTVQARQLFAEARIPTYDTPEKAVRAFLH
                     LIDYSQRQKTLMETPPSIPHEFRPAVSSARLVIAQALEQGRSLLNESEAKAILTAYGI
                     PTVETHIASTAEQAIRLAEQVGFPVALKILSPDITHKTDVGGVVLDVESSQAVETAFQ
                     GMRDRLQHIHPGAEMRGVTVQAMARRPGAYELLIGATVDAAFGPIIVFGQGGIAVELL
                     DDHAMALPPLNMKLANDLIHSTRIVSLLQGFRTRSAVDLEALCLTLIQISHLVQDFPE
                     IVELDINPLLADKNGVLALDARMKIASSRLSGPHHLAIRPYPQELEEWITLQSDEKLF
                     CRPIRPEDEPAHHEFIARLTPEDIR"
BASE COUNT         2110 a         1952 c         2334 g         2137 t
ORIGIN      
        1 tgctcatgaa tcacttttct acatcttcca tattgacgaa agacctgatg aactccgaag
       61 ccatccccct cacgcccagg acatttgtta cgacattgtt tcaccatatc ctcctctgcc
      121 tgtcccaagc cggtttgttg gtgcgttcgg tgtgaacgtg tgcatgatcc ggcggacggg
      181 tcatctcagc gaaaaaggtg gcgggatatc gaaaaatggc gtgaagggtt ggacgtggtg
      241 aagcggcatc gtgtgtatgg ccttctggtt gatatgcaaa tgtccattag agagggtgga
      301 ccatgctggg tgaatcgcga tggttggggc atcaaatgcg ggtgttgatg atgtcgggtg
      361 gttctgacga acgtgtcctc cgcaaactgt tccgggaagg agcgcaaggc tgtatcttaa
      421 aaccgtttca tctcacatct cgcaagcaag tctgtcgcga gatcattacg aaaatggaga
      481 ttgaggaaca ggtcccccat tatttccatg tggcctgatc aaggcgcgga tccaaattga
      541 aaattgaacc cctatgcggg aggcggaaaa tccgtgggag aggaaagtgt gaagtcagtt
      601 gaggtcgatg gcatcaaaaa aaacgggaag agtgtaatct gcgctcatca acgcatggtg
      661 gataatcatt acaacgaaga aggcaggaga atcgggaacc tggtgtgtcg ggaatgtggt
      721 gaggtcattc atgatccagt tgaagcctga taatggaggc gttctctcct gacatgtgat
      781 cgggtctgca taaaagtcaa aacccgtaat acttctgatg acacacgaag ttgaatttgc
      841 caaataaggt gaaaaggtga acaggctggt gaaagcccaa agttattcca tcatgctggg
      901 cttcgatggg tccgatgtgc cgacatttga gcctcacttg tcggtacagg aaatcaattg
      961 tgctgattct gctcggattt ccgactgtct tgaggggaga tacaccttta aagggctcaa
     1021 tcctgcctgg gttcctcata attaatcttg ggtgtgacca acgggtctga ttcccgcctt
     1081 ctcccaatcg ttgttgcaaa cttcaacagt tccccaacat tccctgtagc gttttggtca
     1141 agggacgatc ttccgctttt tgccaacctc tcagaaacca ctataaaaaa agggaattgc
     1201 tcagagcctt tcccctggca taaccctcgc tagaaaaaga tgagcgggac tatatccact
     1261 cagggacagg tgaaatgtgt ctgtggcaat ggaagtggca gtgaagaaaa gtgaaaaaat
     1321 ttaagccatt aaacggtgtg tttttctatg gaaatgagcc agaggggctg gtaacgactt
     1381 tctggctcac aggtgaaagg taatcgtatg tatcggattt tagatgaaag tgcagaagga
     1441 agtgtgggaa tacgggttga gggtaaattg tcgaccgaag agtatgatct attgaataca
     1501 tacttggaaa cgttgatgca ggaggttggg ccgataaatt tcttatgtga tatggcaagc
     1561 tttgagagcc tgagcggaca agccttatgg gaagagatga ctgatcatct tcggtacatt
     1621 catgagtttc agcgaattgc gttggtgggg aatcgacgga tgttgaaggg ggaaccaaag
     1681 atttctgttc cctggcccaa ggtccaattg aaatttttta caccagatca aaccgacgag
     1741 gcctggcatt gggtaaaggg gtgaggtcca caaggctctt accaattggc ttgcgctggg
     1801 ccgcagagga atcgaccggg aatgagtgtg tggcagttcc gggtgaacag ccgatagggt
     1861 tgaaacccct ccttcgcttc ggtcgacggg ctcatagctc agcgagatcc ttcgtttcgc
     1921 tcaagaggac agaataaggg gtcaatctgg aacggtagga cctagggact ttttcctggt
     1981 ttactaggga aattcctagt tccttccctc tgtggaatgt tttacaaaag tttctggaaa
     2041 gaaggggaaa gaccctattt ttcttaggca ttcaggaccc aaggagggga acaacatgct
     2101 gagtacccag gttaatcatt tgtggtccgt tgtcttagct ggtggagaag gggaaaggac
     2161 ccgcccgttt atcgagcaat ggttgggcgg tcataagccc aagcagtatt gtacatttgt
     2221 cggaaaacgc tccatgctcc aacatacgct ggaccgggca gaccgattag taaatcccga
     2281 acataaagtc acggtgatcg ggcggaatca tcgagagttt ttgaatgaag ccatggagag
     2341 agagaagtcc ggacacgtca ttgttcagcc acataatagg ggaacagccg caggagtgtt
     2401 ccttccgctg acctttgttc gggcatggga cgctgatgcc acagtagcca ttttcccatc
     2461 cgaccatttt gtctttccag aagctcgttt tgtcgagacg gtcaggcgtg ggatccgcgc
     2521 aagtcatttt ctccaggatc ggttgatcct ttttggcgtt tctccttcac atcctgaatt
     2581 ggactatggg tggatcaact tgagcgaagt cctgggctgg agcagtgggt cttgtattcg
     2641 cctggccggg tcctttcttg aaaaaccaga catcattgaa gggaaacagg caatggccaa
     2701 tggagcgctt tggaatacat tggtgatggt gggaaaagtg gcaactctct ggaaacttgg
     2761 ttggcaatgc cttccggtta tgatggagcg gtttgaacag ttaggttcga tgattgggac
     2821 cgttcacgaa aatgagacgc ttcatgagat ctatcgggac atgccggttc tggacttttc
     2881 ccgtgaattg ctgcaacgga ttcctgagca tctgggagtg atggaattcg aagaagtgct
     2941 gtggagcgat tggggacaac ccgaacgtat agtccagacc cttaaaactc tggggaaaga
     3001 gcctgcattt ccttccgata ttttacaaag gtctttggct cccatacaga caattcctcg
     3061 tgtgggggtg gcctaatggc gaaaaaacga tcggttcaac catccgatga gacaccccga
     3121 cagtctcctt ccgccgccaa gcgaaagagc gtatcgagga aaaagacctc tatgagagag
     3181 gaccctttgg aggttcagga gagtatgatc cctggcgata ccgattctat gaactctcaa
     3241 cctaccaatg ggaatgtgca tgcgcgtatt gcccggcggg cgtatgaatt atttcactct
     3301 cgaggggggc accatggaca ggatctcgat gattggttag ttgccgagca agagatagag
     3361 gccaaagata gatcatcata gcccaggagg tctattcctt agctgagttg cagaatcctg
     3421 gttggcactt cttcctcttt tgatctgaat gacggccccg tttcctataa tatttttccc
     3481 tgtttctgta ggtcgattgg cgctgatcca aaatgatgtc gctttcatcc tttctaaaga
     3541 ggaggtgttt atttgatatc caggaagatg ttctgtggtt tcattttcat atcgtgggtt
     3601 ctaccaatcg gtgggctttg caagtcttat ttggtctcaa gatcagggac gtgaccaact
     3661 ccaggttcag gattaccttt aacaacggga aaaaggattc attcccaaaa tcaacgggaa
     3721 atggcaaacc tccatcaatg ggaagaggac gtgatatgtg tcgtcaagga cgggaaaacg
     3781 taaggcccat tttcggctga aatggatgaa ccaaaaatcc atgagccaaa gattactgcg
     3841 cagaacaatg agagccaaac gttcatcacg taagcattga aaggttccgc accgttatcg
     3901 gtttcccctc acgtgcggaa ccccttcccg gattttgttt gattgattga gaggctgact
     3961 tgtggggatg ttggacgcat gaggcctctc acctgcttat ccctcccttc tttttctggt
     4021 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccattggc aataatctct
     4141 accgcgatgg ctgccaggat aaggcccatg atacgtgtaa aaatattaat gccggtcacg
     4201 cctaaacgtt gtgagatcag gggagccagg tgtaaagcca tccataggca aaggccaata
     4261 attatgatga tcaaacctag caacaattca tgccccaggt tggtgccttt atgtgcttca
     4321 accacaactg tactaattcc accgggaccg gccagcaatg gggttgctaa cgggacgacg
     4381 gcaatatcgt gtttcttgtt ggactcttcc gcttcttctt tggtttgcac tgtggggctc
     4441 aattctgctt gaagcatgga aatagccatg agtaggatga ggatcccgcc gcctactcta
     4501 aaagaactca cactgatccc gaaaatttcg agcatccatt ccccagcgat taaggccatt
     4561 aacaaaatca gggtcgcaga gaatgccgca attttggcaa tcttcttccg agcggccttc
     4621 tcttttcctg gtgtgagtgc gatgaaaata gagagagccc ctaaaggatt gatcacggct
     4681 aagagcgcca cgaagatttt tacgtattcg gtgtcatcaa gcatgcgtta tcgatatcca
     4741 ttcatgacca accgtttcgc atcgtaggca ggtatccaaa gggtggctga aaaattatgg
     4801 gacttcaacg gacatgaggc tgattgatac gacggacaag gacggtattg ttatgatcta
     4861 ttctcaagaa agatgctccc caattccacc ccaccgcatt gtaaccagtg gggagtgact
     4921 ctgcacctgg catgctcctc ccggctggtt cgggagaatg aggaagatcc ttgaagaccg
     4981 actgagtcga tgttgtgggt gtttctttat ggagatctgt cggaatttga ggaagacgac
     5041 aggcggtttt tgcgcaactt ccagaatgga agagacgttc tcaagcggag gagggaagat
     5101 tgatgaatca gtctttggcc actgagtaga gaatgagggt gtcatacgat agactaggcc
     5161 gttcaggtcg cattccaagt aaaaatttga aaggagccca atgatgaaag tgctgttagc
     5221 ggtagagcaa actcgtattt ctgtaggtgc tattcggata ctcaccaggt tatctcttcc
     5281 cgttggttcc aatctgtttt tactccatgt ccatcccgtt cctcaaaaga tcatggggct
     5341 ggcaaaggaa aggattttga aaatctctca gcaggttcaa gaggttcagg gagaagtcca
     5401 tgaacgggcg cggcggtttt tgggcaaagt ccagaacacg ttacggcggc aggatgtggt
     5461 gtttcactct ctggtgaagc atggatttcc cggagaagag attttaaaaa ccattcgcac
     5521 caaaggcatc gatctggtgg ttctcggtac acggagctat tccaaagctg ccggattttt
     5581 tcttggcagt gtgagccaat gggtgttaca ggaagctcca tgctcggtgt tgattgcgcg
     5641 aaatacgccg cacagatcac tgaatgacgg agggatgaaa atactgctgg ccactgatgg
     5701 atcttccgat tcacgggcgg cagtcgattt tgtcaaagag atgggtttgc cggattcctc
     5761 gcatatgatg attctccatg tgatcaaaaa acacatgtat gaaaccgaac gggcgttgac
     5821 taccggcagg gaaaatgaaa aggaatttgc aaagctggca gaagaagttt tggaaataca
     5881 agagcaacag gggatgaaac ttcttgagca gacgtcagcg gtactctctt cgcctgacct
     5941 cacgacgcaa aaaaggctgg tctatggcaa tcctgcgacc gaaatattga aaaccgcgcg
     6001 aatgatgaag gcggatctta ttgtaatggg ctcccaagga tcgacggggg tcaagcggat
     6061 gattttgggc agcgtgtcca acaaggtggt ccgtagcgcc ggatcctcgg tggcggtcat
     6121 tcgcagtaaa aggaaaaaat aattttcgga agcgtgagca agaaagctgt cttcttgtat
     6181 gagccatatc ctggtggcgc ccgtttgtgc caacagaagt gcacaggaga atagttctgt
     6241 ttctcacttc ctcacacgac cccgtatcaa ctggcgccgt cggcattgtc aagagatttg
     6301 aaaatattta catgtaaata cagtaaaggc acgtgtcatg agcattcgaa atttcaagta
     6361 cctgtttaag cctcgttcta ttgctgtgat cggtgcgtca aatcggcctc atcgagtagg
     6421 acgtgtcgtg atgcagaatc ttctgaaagg gggattcgag ggccccctac tgccggtgaa
     6481 tccaaagtat caagccgtgt gtggagtact ggcctacccc gatgtacagg ccttgccgat
     6541 aagtcccgat ctagccattt tgtgcacccc accctcgacg atcccccggc tgattcaaga
     6601 attaggggaa cggggagtcc gggctgcagt cattctgaca gccggcatga gaccgaagcc
     6661 atcagaaaag gaaagcgaca ccgaatggac gatcatgctt gaaactgcac gaaattttgg
     6721 tatgcgtctt cttggtccga ataccatcgg tctgctgatt cccgggctgc atctgaatgc
     6781 cagttttgcc catatggaag ctcttcccgg atcgctggca tttgtttctc agtctggtgc
     6841 actctgcacg gctgtgttgg attgggctcg ctctcaagga atcggctttt cccatttttt
     6901 gtctctcggg gacagcgccg acgtcgatat gggcgatgtt ctggattatc ttggcagcaa
     6961 ccccgatacc cgggcgattc ttctgtacat tgaatccatc acacaggcga gaaagttcat
     7021 gtctgcagcc agagccgccg ctcgcaataa accggtgtta gcaatcaaag ccggtcgaca
     7081 tccggaaggg gcgacggccg ccagttcaca taccggagca ctcatcggtc gtgacgaggt
     7141 gtacgatgcg gcattctgcc gggcgggaat tttgcgggtg tttgaaatcg atgaactgtt
     7201 cgatgcggtg gaaacgctgg aacgagccgg cccgttgcgg ggagaacgat tagcgatcgt
     7261 cacgaatggg ggcgggccgg gagttctggc cgccgatgct tttgccgaag ggggtggttc
     7321 tctcgcccgc ttgtctgagg ccacgattca tgaactcaca acgttgttac cggcgagctg
     7381 gtctcacgga aatcctgtcg acatcgtcgg ggatgcgaga gcggaacgtt atgctcaggc
     7441 tttgaccagt atcctgaagg ctccggatgt ggatgcggtc atcgtggccc atgcccccac
     7501 ggcagtgatc accagcgagg aggccgcatt ggcggtggtg aatgcggttc gcgcatcgtc
     7561 ctccaaaaaa aatgtattca ccagttggct cggaggccaa acaaccgttc aggcccgcca
     7621 actgtttgcc gaagcccgga ttccaacgta tgacactccc gaaaaagccg tgcgggcgtt
     7681 tctccatctc atagactact ctcaacgcca aaaaacgtta atggagaccc cgccttctat
     7741 tcctcatgaa tttcgcccgg ctgtctcttc cgcgagattg gtgattgcac aagcccttga
     7801 gcaggggcgg tctttgctga atgaatcgga agccaaggcg atcctcaccg cctatggtat
     7861 tccaacagtg gaaacacata ttgcgtcgac ggctgagcaa gccattcgtt tggcggagca
     7921 ggtggggttt ccggttgcgt tgaaaattct ttctccggac attacccata agacagatgt
     7981 gggtggagtg gtgcttgatg tggaatcttc tcaagccgtt gaaaccgcat ttcaggggat
     8041 gagggatcga ttgcaacaca ttcatccagg ggcggagatg cgtggagtca cggtacaggc
     8101 gatggcgcga cggcccggcg cctacgaatt actcattggc gcgacggttg atgccgcgtt
     8161 tggcccgatc attgtcttcg gacagggagg gattgccgtc gaactgttgg acgatcatgc
     8221 gatggcgctt ccgcctttga atatgaaatt agcgaacgat ctgattcatt cgaccagaat
     8281 tgtctcatta ttacaggggt tccggacacg ctcagccgtt gatcttgaag ccctctgcct
     8341 gaccttgatt caaatttctc atctggtgca ggactttccg gaaattgttg agctggatat
     8401 caacccgctt ctcgccgaca aaaatggggt gctggcgttg gatgccagga tgaagattgc
     8461 gtcttctcgc ttatcgggtc cgcaccatct tgccattcga ccctatccgc aagagcttga
     8521 ggaatggatc acgctccaat ccgatgaaaa actgttctgc cgtcctattc ggcctgagga
     8581 tgaacctgcc catcatgaat ttatcgcccg attaactccg gaggatatcc gat
//
LOCUS       DAORAQ010000114         8491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1320_length_8491_cov_61.348151, whole genome shotgun sequence.
ACCESSION   DAORAQ010000114 DAORAQ010000000
VERSION     DAORAQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_8491_cov_61.348151"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1595)
                     /locus_tag="PKM72_10045"
     CDS             complement(117..1595)
                     /locus_tag="PKM72_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HNP61172.1"
                     /translation="MEPEVYAADDGLPYPLGSVRDVLTALFKHKTKVLTIFLLVVGIV
                     AGYVFTLVPLYEAKTSLLLKLGREHVFRPEVGKEGQFVKYNEDAALQSELEIISSKDL
                     IRRVVSTLGIEKVYPEFLDPSLEIHDPLEKGVSTFLKNLLPIPVKGSSVLEISYLNPR
                     PEVAAEALNLLVELLKEKHLQVFSDPKASFLTKQLHTYQEKLEQSEANLQAFKHRHDL
                     SSPLVEQQRRLLDQRATLDSDYKTTKNKLQGLVGKIASLDTQMKTILESISDSTVEEG
                     GNLEKAKAELFALRREEQKLLAKYTETSFPVLSLRTEIDQVEQFILAEQKNERANTIA
                     SGKKAMYSELEKERLGAVSESTTLQASREVIALQMEDLDKKIQRLDELNKEMIVLERQ
                     RAADEQNYNLYLSRVEEANVSEEMDRLKMANISVIQRAEVPGGPAGRSPILLVTLGAI
                     LGIMAGAGFGLLLEYFQGAYTRPEQAANDLNLPLLATFGQKL"
     gene            complement(1599..2759)
                     /locus_tag="PKM72_10050"
     CDS             complement(1599..2759)
                     /locus_tag="PKM72_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNP61173.1"
                     /translation="MNITNIQLAYRWFYRHSLIRPNTVPVLLALVLCGGISVHQTPAY
                     ADPGSVVIGKASNPEDIEKIENEVFSIMKQQLRSYQQSLEESEGRLQDFQRKHGIILI
                     ETQMNHLLQQRLSLDDSLKQAENQVKGFQERLAWVQGQISQIPEQIPLSSSASEQGVV
                     GGAKNNLLTLQLKEQELLNKYTENNPHIQSIRKEMELINQFIKEQESQVARSVSTGKN
                     PLYLEMEMQLFHTQADLVSAEAQSEVIKQQIAGVDKELDRLRNLGPELDELRRQVTAD
                     EKNYINYLTKVGKTPPQDYQIQVGDQLDIKFFFNPELNETVLVRPDGRIALQLVGEIT
                     VVGHTVEQIRAILIKTYSGQLKNPEVAVLLRSTNVPGEDSGTTAAEGNFGGK"
     gene            complement(2887..3582)
                     /locus_tag="PKM72_10055"
     CDS             complement(2887..3582)
                     /locus_tag="PKM72_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNP61174.1"
                     /translation="MRILFVVATFILLCGCNSGWKGMKTVDASNLEPNGYPALSGKTK
                     VDPPSLVPGQEHEKINVDDPRYIIQPGDQLSVKFFFNPELNEEDLVVRPDGHISLQLV
                     HEVKAADLTPIQLKSILEEKYVGQLKNPEIAVIVRSVREPYRIYVDGEVKTPGEYEMV
                     GSLSVLQAIARAGGMKEDTAKKGDVKVIREDQDGQRFVINVDLDRALNGGDLSQDIRL
                     LPSDMVYVPRSFW"
     gene            complement(3996..6536)
                     /locus_tag="PKM72_10060"
     CDS             complement(3996..6536)
                     /locus_tag="PKM72_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNP61175.1"
                     /translation="MTKRVVDTLGAFFGLVVCMPLFVLLAFLIKVNSRGPVLSCQERV
                     GRGFRPFCLYKFRTIEQDVSAKGGELTIGEDPRVTSIGKYLRQLKLDELPQLFNVLIG
                     DMSLVGPRPEVAYYVEKFKEDYHDVLTVRPGLTDLASLKYIDEKSVLGKVEKPEDEYT
                     NRILPEKIRLAKLYIEHASFAFDLAVIAQTLLQLLGLRSVILKVTDRDNHDEVAFLEG
                     SVVFQLITKYRRPIIITLDLGLIVLANYLAFWLRFDGHLPQDATQLLLKTLPGLLVIR
                     GIAFGVFRLNEGLWRYISIWDLKKITGGVLAGTVVFYGFVNWGLGIPGYPRSIFIIDS
                     ILLVGFLCGIRLAVRIFRERKVLRKTKRVLIIGAGDAGENIVREMQSHSTSSYTPIGF
                     VDDDVSKLSKRIHGVKVLGTRQDLSRILHTHLPEEVLVALPGTNSAVLRDITALLAPF
                     NVRIKTLPNLGDILEGKVTINQIRDLAIEDLLQRPPVGLDPQPVRNLIDGKRVLVTGA
                     GGSIGSELCRQIIALGPKTLILYERHENSLYAIAGELTDQGHAGVIHEVLGDITDVPR
                     LHETFKTYRPEIIFHAAAHKHVPLMEANPGEAFKNNVLGTRLVAEAAAHFGVEHFVLI
                     STDKAVNPSSVMGATKRVAELVVQAMASQNQSQTRFLTVRFGNVLGSNGSVVPRFQKQ
                     IKAGGPVTVTHPEVRRYFMLIPEAVILVLQAAALGEQGAIYVLEMGGQIKLVDLARNL
                     IRLSGHVPEKNIPIQFIGLRPGEKLEEELVGECERAVPSSIDKILRIQSIQSLDQAFL
                     TNILSKLEDVQILGHSPSVIELLQQLVPTFHRPEILELAAMSEEYQIH"
     gene            7134..7274
                     /locus_tag="PKM72_10065"
     CDS             7134..7274
                     /locus_tag="PKM72_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61176.1"
                     /translation="MLKERSKPEKQERGRNDALMDLIQSGAQQLLAHALNAEVAGLVA
                     TT"
     gene            7367..7660
                     /locus_tag="PKM72_10070"
     CDS             7367..7660
                     /locus_tag="PKM72_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61177.1"
                     /translation="MRGPLLVVPSHPSSDPLLGFTYNKHPNIAWKYKLIRRDTVTNAT
                     IHDSQALDEVLLAKTAGCDVWADAVYRFQAIEAKLKRRKLRSRVQYKGYRIPF"
     gene            complement(8230..>8491)
                     /locus_tag="PKM72_10075"
     CDS             complement(8230..>8491)
                     /locus_tag="PKM72_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61178.1"
                     /translation="IFKPHRSHLYQYLSNEMGKSHLIISSVYGLLQLAVNAILILFLP
                     NSSVGLVVLFATIFVVLYVVVRERVLSRLGIKGFVGDSKLFV"
BASE COUNT         2376 a         2090 c         1766 g         2259 t
ORIGIN      
        1 cccccatcgc ttccttatgg ctggggctca gataaaggaa atttgggtca ggagtggtat
       61 gaaatggttc ttttttgagt ccgtaaaatg ctagatacat agtgataaat ttcttattac
      121 agcttttgac caaaagtggc caggagggga aggtttaaat cgtttgccgc ttgttcaggg
      181 cgagtatagg caccttggaa gtactctaat agaagtccaa atccggctcc agccattatc
      241 cccaaaatgg ctcccaacgt tacaagcagt attggagaac gtccagctgg gcctccaggg
      301 acctctgcgc gttggatcac gctaatattg gccattttga gccggtccat ttcttcagat
      361 acattcgctt cctctactcg agaaagatac aaattgtaat tttgctcatc tgctgccctc
      421 tgccgctcca ggacaatcat ttctttattt aattcgtcaa gtctctggat ttttttgtca
      481 agatcctcca tttgaagggc aatcacctca cggcttgcct ggagggttgt tgactcggac
      541 acggccccca gtctctcctt ttcaagctcg ctatacattg ctttctttcc acttgctatt
      601 gtattagcac gctcgttttt ttgttcggca agaataaact gttccacctg gtcaatttcc
      661 gttcgaaggc ttaaaacagg aaaactagtt tcggtgtact tggctaaaag cttttgttct
      721 tcacgtctca gagcaaacaa ttccgctttg gccttttcca ggtttccccc ttcttccact
      781 gtggaatcag aaatactttc gagaatggtt ttcatctgag tatcaagtga agcgatttta
      841 cctaccaatc cttgcagctt attcttggtg gttttgtaat cactatccag agtggcccgc
      901 tgatccagga gacggcgttg ctgttcgaca agaggggagg aaagatcatg cctatgttta
      961 aaagcctgaa gatttgcttc tgactgttcc aatttctcct ggtaggtatg taattgtttc
     1021 gttaaaaatg aagccttcgg atcgctaaac acttgaagat gcttctcttt cagtaattca
     1081 acaaggaggt tgagtgcttc agctgcaacc tcaggccggg gattcaaata cgatatttca
     1141 agtacgctcg aacccttgac ggggatgggt agaagattct tcaaaaatgt tgaaaccccc
     1201 ttttctaaag ggtcgtgaat ctccaatgaa gggtctaaaa attccggata gactttttca
     1261 atacctaaag tgcttacgac ccgccttatg aggtccttac tagaaataat ctctaattcc
     1321 gattgaagag cagcatcctc attatattta acaaattgac cttctttccc tacttcagga
     1381 cgaaatacat gctccctccc aagcttcaaa agtagactgg ttttagcttc atagagaggg
     1441 actaatgtaa agacatagcc tgcaacaatg ccaacaacca acagaaaaat agtgaggacc
     1501 ttagtcttat gcttaaaaag agcggtcaga acatctcgta cacttcccag ggggtagggt
     1561 aaaccatcat cagccgcata gacttcaggt tccatatgtt atttccctcc aaaattccct
     1621 tcagcagctg ttgtgccgga atcttcccct ggcacatttg ttgatcgaag aaggaccgcc
     1681 acttcaggat ttttgagttg cccggaatat gtcttgatta atatcgctcg aatttgttcc
     1741 acggtatgtc caacaacagt aatttcaccg accaactgca aggcgatacg cccatcagga
     1801 cggaccagaa cggtttcatt gagttccgga ttgaaaaaga atttaatatc gagctgatcc
     1861 ccaacctgaa tttgataatc ttgtggtggc gtcttcccta ctttggtgag ataatttatg
     1921 taattttttt catctgctgt gacttggcgc cgaagttcat cgagttccgg ccccaaattt
     1981 ctcagcctat ccagttcctt gtccacacca gcaatctgtt gcttaatcac ttcactctga
     2041 gcttcagctg atacgagatc ggcttgggtg tgaaataatt gcatttccat ctcaagatag
     2101 agcggatttt tgccagtgga tacactacgt gccacttgag attcctgttc ctttatgaac
     2161 tgattaatca attccatttc ttttcgaatt gactgaatat gagggttatt ctcggtatat
     2221 ttgtttaaaa gttcctgctc ttttaattgc agggttagta aattattctt ggccccacca
     2281 actacccctt gctctgaagc cgaactggaa agagggatct gttctggaat ttggcttatc
     2341 tgaccctgca cccaagcaag tctctcttga aagcctttga cttggttttc ggcctgcttt
     2401 aatgaatcgt ccaaagataa acgctgttga agaagatgat tcatttgcgt ttcaattaat
     2461 ataattccat gcttccgttg aaaatcctga agacgcccct cggattcttc caggctttgt
     2521 tgataagaac ggagttgttg tttcataatc gaaaatactt cattttcaat tttttctata
     2581 tcttcagggt tcgaagcctt tccgattact actgatccgg ggtcggcata agcgggtgtc
     2641 tgatggacgg aaattccccc acagaggacg agtgcgagta aaacaggcac agtgtttggc
     2701 ctaatcagag aatgtctata aaaccatcga taagctaatt gaatatttgt aatattcacg
     2761 gtattccttt cattcctttt caaaacaaat ctctcttaat ggcattaatc tccattgtta
     2821 atatagaaaa ggattgagcc tggaaatgca ggcaacattc ttacttacac aagcaaaccc
     2881 attgacctac caaaatgacc ttggcacata gaccatatca gacgggagta gtcgaatatc
     2941 ctgactcaaa tcccctccat tgagagccct gtccaaatcc acattaataa caaatctctg
     3001 accatcttga tcctcacgta ttactttcac gtcgcctttc ttagccgtat cctccttcat
     3061 acccccggca cgcgcaattg cttgcaacac acttagtgat ccgaccattt catattctcc
     3121 cggtgttttg acttccccat cgacatagat tctataaggc tcccggactg aacgaacaat
     3181 gacggcaatc tccggatttt tcagttgtcc aacatatttt tcctccaaaa tgcttttaag
     3241 ctgaatgggg gtcaaatctg cggccttaac ttcatgaacc aattggaggg aaatatgccc
     3301 atcagggcgc acaactaaat cctcttcatt taattccgga ttaaaaaaga attttacgct
     3361 taattggtcc ccaggttgga tgatataacg tggatcatcg acattgattt tttcatgctc
     3421 ttgcccagga actaaagatg gaggatcaac ttttgttttt ccagaaagag cagggtaacc
     3481 atttggttcc aaattcgaag catcaacggt tttcattccc ttccaccctg aattacagcc
     3541 acacaggaga ataaacgtag ccacaacaaa tagaattctc atcctcttaa gttccttcat
     3601 tagtagcaaa ccctctctag ataccccaat taacaccatc cgaccagggg aaaaatttaa
     3661 aaaagccttc tcgactcact taagcatatg tttctaaacg atatttcatt ttttaccgct
     3721 attaacgaag gtaaatttac ttaccacagc aactgaccct tgtagacaag ggtaatctct
     3781 tcacaacttt ttaccctaac taaaaacatt aacttataaa aacaattttt atcaagatca
     3841 agcaaactta aggctttatg ccctctcgga taaatcaaac tgaaaattaa gaataacagg
     3901 aaatttggat cctatgggct ttcaagcaga atcgagaatc aacctattgt cacccactat
     3961 ccgtcaaact cgcgggtttt agctaggcaa tcttatcagt gaatttgata ttcctcactc
     4021 attgctgcca gttccaaaat ttcaggacga tgaaaagtcg ggaccaactg ctgaaggagc
     4081 tcaatcaccg agggagaatg gcccaatatc tgtacatcct ccaacttgct cagaatattt
     4141 gtcaggaatg cctgatcaag ggactggatt gattgaatcc tgaggatctt atcaattgat
     4201 gaaggcacag ctcgctcaca ttccccgacc aattcttctt cgagcttttc tccaggtcga
     4261 agaccgataa actggatggg aatatttttt tcaggcacat ggccagagag tcgaatgagg
     4321 ttacgcgcga gatccacaag tttgatttgt ccgcccatct cgaggacata aatagcgcct
     4381 tgctccccaa gggcagcagc ctgcaatact aagatcaccg cttcaggaat cagcatgaaa
     4441 tagcgccgaa cctcaggatg tgtgaccgtg actggtcccc cagcctttat ctgtttttgg
     4501 aagcgcggca cgacacttcc attactgccc aaaacattac caaagcgaac ggtcaggaat
     4561 cgcgtttgac tctgattctg actcgccatg gcctggacaa ccaattccgc cacccgtttg
     4621 gtcgccccca tgacactgga tggattgaca gccttatctg ttgaaatcaa gacaaaatgc
     4681 tcgacaccaa agtgtgcggc cgcttcggcc actaaacgtg tgcccaagac attattctta
     4741 aaagcttctc cgggattcgc ttccattaac ggcacatgct tgtgggccgc cgcatggaat
     4801 atgatctcgg gacggtacgt cttgaatgtc tcgtggagac gtggaacatc cgtgatatct
     4861 cccagcactt catgaatcac accggcatgg ccttgatccg tcagctcccc cgcaatcgcg
     4921 tataaactgt tctcgtgtcg ttcatatagg atgagggtct taggccctag cgcgataatt
     4981 tggcggcaga gctcagaacc aatagacccg cccgccccag tcaccaatac tcgcttccca
     5041 tcgattaaat tgcgaacggg ctgggggtcc aaccccacag ggggacgctg aagcagatcc
     5101 tcaatcgcga gatcccgtat ctgattgatc gtgactttgc cttccaggat atcacccaag
     5161 ttcgggaggg tcttgatacg cacgttaaat ggcgccaaaa gtgccgttat gtcgcgaaga
     5221 acggctgaat tcgtcccagg caaggccact aagacttcct ccggtaagtg cgtgtgaaga
     5281 atgcgtgata gatcctggcg tgttcccaga acttttactc catggatgcg cttgctcaat
     5341 ttggagacat catcgtccac aaatccaatg ggcgtatagg atgaggtgga atgtgattgc
     5401 atctcccgaa cgatgttttc accggcatca ccagccccaa taatgagaac acgtttcgtt
     5461 tttctcaaaa cctttcgctc ccgaaagatt cggacagcaa gccggatccc acaaagaaat
     5521 ccgactaaaa ggatactatc aatgataaaa atggaccggg gatagccggg aataccaagc
     5581 ccccaattga cgaagccata gaacaccacg gtcccagcca ataccccacc cgtaattttt
     5641 tttaaatccc agatactaat ataccgccac agcccttcat tcaggcgaaa aaccccaaag
     5701 gcaatcccac gaattacaag cagacctggc aaagtcttga gcaaaagttg cgtggcgtct
     5761 tggggaagat ggccgtcaaa tctcaaccag aacgccagat aattggccaa cacaatcagg
     5821 cccagatcaa gcgttatgat aatgggacgc cggtatttgg taataagctg gaaaaccacc
     5881 gacccctcaa gaaaagccac ttcatcatga ttgtcacgat cggtaacctt taaaattaca
     5941 ctccgtaaac ccagaagttg caatagcgtc tgggcaatga cggcaaggtc aaaagcaaaa
     6001 gaagcatgtt caatatatag ctttgctaag cggatttttt ccggcaggat cctattcgta
     6061 tactcgtctt cgggtttctc gacttttccg agcacgctct tttcatcgat gtatttcaga
     6121 gaagccaggt ccgttaaacc aggccggacc gtcaagacat cgtgatagtc ctctttaaac
     6181 ttttcaacat aataggccac ctccggacgt gggcctacca gactcatatc ccctatcaaa
     6241 acattgaaca gctgcggaag ctcgtcaagc ttcaactggc gcaaatactt tcctatgctg
     6301 gtcacacgag gatcttcgcc aatggtcagc tcccctccct ttgcagagac gtcctgctca
     6361 atcgtgcgaa atttatacag acaaaatggc cgaaaccctc gtcccacgcg ctcctgacag
     6421 gacaacacag gcccgcgaga gttcacttta ataaggaagg cgagcaggac aaagaggggc
     6481 atacacacca ctaagccaaa aaacgcacca agagtgtcaa caacacgctt agtcatcacc
     6541 ggtactcctg aatgaacatt gaacccactt gaatggaatg taaattttga tctctatcat
     6601 tttccaatta gatagaaaag gagagctacc accacaagga gggggggaac agaccatgta
     6661 tccactagaa agcttcaaga atctgagtct caatgcctcg attacttatc gccagtgcga
     6721 tttgataagg gttgcgacaa cctcaatgac atattggata tgaccttctg gcattttggg
     6781 ataaatggga agagaaataa ttcgttcaaa tacggaacta gccacagaga aatctttggg
     6841 gagataccgg taattatccc tataataggg gtgaagatgc aagaggataa aatgcacact
     6901 ggttccgatg ccctgtttat tcaaaaatct ataatttggt tctatccgaa ctataatatt
     6961 cctaggccta aatgtaataa tggaaggata tcagctgtac tcaacgttct ccatcacagg
     7021 gtgaatcgta atccgagcta aacccgaaag tagtgtttga cttgaattga aaagtcccag
     7081 cctctcggat ggccatgggt gttggaagac gaccaccacg aatgagaaac gccatgttga
     7141 aggagcgtag caaaccagag aaacaagagc gaggaagaaa cgatgcactg atggacttga
     7201 ttcagagtgg agcccaacaa cttctcgccc atgcccttaa tgcggaagtg gctggactag
     7261 ttgcaaccac ttgaaactat tgaggttaat gtaatactcc acagaaatca agccacctgt
     7321 taaccttttt ctgcctacat tgacaagaac tctggccctc tgacgcttga gagggccgct
     7381 cctcgttgtc ccatcgcatc catcaagtga tccgttgctg ggcttcacat acaacaaaca
     7441 tcccaatatt gcttggaagt ataaactcat tcggcgcgat accgtcacca acgcgacgat
     7501 acatgacagt caagccctcg acgaggtttt gctggctaag accgctggct gtgacgtctg
     7561 ggcagatgcg gtctatcgtt ttcaggcgat cgaagccaaa ctgaaacggc ggaagctgcg
     7621 ctctcgggtt cagtataaag ggtatcggat acccttctga tggcggctca acatcagacc
     7681 agttggcgtc ggccgcgcgt ttgagcccgc gtcgaacata actttggcca tcatgtcacg
     7741 ccgatggagg gcaaggtgct tcccacaatc gggcttgtcc aggctcgggc caaaattggg
     7801 ctccggaatc tggcgtataa ttttcaccga tttcttctgt tcactcttcc ggcccaaggg
     7861 caattgcttt aatcaatgag ccaaggggga accatgggcc ggaacccaga tgtcaggaga
     7921 ccgctcgcat cctttccccc cgtaggaccg tttccataat gggttcgctt acccaatgac
     7981 aaataaggcg accttcagat tttttgaggt gcccttatgt cagtttgaga ccccagcatg
     8041 atcatcctta atcgaacatg tcgaaggcaa aattctcaac tgcccagcgg cctgtatttc
     8101 cggggtcggt caccagtcag aaaatccgct ctctcctctc ttcaactggg ttaaaatgag
     8161 aggagctacc aacactcgaa ggacctatat atggaaaata acccaagaac ggtgacagct
     8221 caccgccgct tacacaaaca acttggaatc tcccacaaac ccttttatac ccaatctaga
     8281 taaaacccgt tcacgaacta caacatacaa aaccacaaaa attgtggcaa ataatactac
     8341 tagcccaacg gaactattag gaagaaaaag gattaatata gcattaactg ccaactgcaa
     8401 tagtccataa acactggaaa taatcaggtg tgatttcccc atttcattcg aaagatattg
     8461 gtataaatgt gaccggtgtg gtttaaatat a
//
LOCUS       DAORAQ010000115         8382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1343_length_8382_cov_66.383091, whole genome shotgun sequence.
ACCESSION   DAORAQ010000115 DAORAQ010000000
VERSION     DAORAQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1343_length_8382_cov_66.383091"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..642
                     /locus_tag="PKM72_10080"
     CDS             274..642
                     /locus_tag="PKM72_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNP61179.1"
                     /translation="MKNASKTSSRPKRGSAKSSPYHLTLYVAGHSPKSRLAMSNLQQL
                     CDTHLSSKYTVEIVDLLQEPKRARLDQILAIPTLVKKLPPPLKKIIGDLSNVEKVLMG
                     LDLIDESGEVLMSSSMKVSS"
     gene            639..989
                     /locus_tag="PKM72_10085"
     CDS             639..989
                     /locus_tag="PKM72_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNP61180.1"
                     /translation="MKSHAPTTPRKVPPGGKPSGMRLRLYVTGATSLSLRAMANIKVI
                     CREHLDEQNSDLQIIDLYRNPAAARANHIFAAPTLIKEFPLPCCRIIGDLSKKDRVLH
                     ALGLTPPLPLPSSG"
     gene            1006..3120
                     /locus_tag="PKM72_10090"
     CDS             1006..3120
                     /locus_tag="PKM72_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61181.1"
                     /translation="MAPKHKPSLAKSRLSSREEILQLRLKEAEDTLQAIREGAVDALI
                     VETGEGDQVFTLKSADYPYRLIIDQMMQGAVIFDESGIIFYANTTFASMVNHSHQELF
                     GFPIESFIAEDDREFFLTLAGSHSPVSGRTGTIHLVSQDHYDLAVFIGMTSIHIDNQH
                     LHCAILTDLTEQKKYDRALAEEKFSRMILEQTGEAVVVCDRSGRIIRHSRAIDRLIQQ
                     PILHTRFHEHFHFTFIQTPVPSGGREPWNEEGNVVQEVLGGRTLRGLEGNLQIANRNI
                     PVLLSGSPLQDDEVNTVGAIFTIVDITERKMAERDLQVFNNSLEQRIQARTQDLLSYQ
                     KHLREMTSELVVTEQRERRRLASELHDYLAQLLVVCRMKLAQQNREAWSPELKTDIEE
                     IDTILSDSLTYTRTLIAELSPSILYELGIVPALVWLGGQFERHGLQVDIQEEDKNIQL
                     PEDHAIFVFQAVRELLFNIKKHADTTQARVCLKKTASHEFQVKVEDTGKGFPLSDASH
                     DYSSPGKFGLFSIRERVEALGGSIDIKAEVGQGTCITLVVPFHSPISSEASLDTKAKS
                     VTSLASFPSNTPQKKGTRILLVDDHALVRQGIRGILEVQADFQVVGEAQDGLEAIEYA
                     RTLHPDLIVMDVNMPKMNGIEATRRIIQEFPLMAIIGLSVQEDKHVQELLLKAGAGMF
                     LTKNGIASELVGSIRQMVAHTP"
     gene            complement(3319..4413)
                     /locus_tag="PKM72_10095"
     CDS             complement(3319..4413)
                     /locus_tag="PKM72_10095"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNP61182.1"
                     /translation="MKMFTCHCGNTLFFENIHCLSCNSEVGWCPACHRMSGLLVSPDG
                     AYRCGYTACHAPLLKCHNYVVEQVCNRCLRASPNDQLVTQLCDYCRYNDTIPDLTVEG
                     NRERWYRLETGKRRLLYTLDLLGLPYGKAEDGIQPALSFDFKADVSLSGNHWHTMGDH
                     EQVFTGHQNGKITINLREAEDVEREKLRVSFGEAHRTIIGHFRHEIAHYYWEMLIQDK
                     QEETFNRIFGNYQQPTYAQALERHYREGPPSDWQANYISVYATMHPWEDFAETFAKYL
                     AIVSVLDTARHTGIGEGIDPTKADIGDMIRSYQRLGLGMNEMNRAMGLIDLMPEVLVP
                     PVIAKLQYIHQIILEARSGMSIPPATRSST"
     gene            4412..>4614
                     /locus_tag="PKM72_10100"
     CDS             4412..>4614
                     /locus_tag="PKM72_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61183.1"
                     /translation="MVIYSRLSVNVRIQARGRKTLHPAVQTAIIMNQKIDTPVLVVNN
                     SSAYCPANELPFSFNPGDHTATV"
     assembly_gap    4615..4714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4715..5055)
                     /locus_tag="PKM72_10105"
     CDS             complement(<4715..5055)
                     /locus_tag="PKM72_10105"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNP61184.1"
                     /translation="MNYQAILNEIHQEVLNLLPCGTVATYIPQLASVSPQNFGMAVQT
                     VDGDVFQVGQALEPFSIQSISKVYTLTLGLPFEGDNIWTRVGLEPSGSTFNSLVQLEY
                     EHGIPRNPFINA"
     gene            complement(5135..5551)
                     /locus_tag="PKM72_10110"
     CDS             complement(5135..5551)
                     /locus_tag="PKM72_10110"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNP61185.1"
                     /translation="MKLRRPQDTLASCCWLARMTDKVRACQQRNFPLLYRISLGSPIG
                     IDGYFLRHFGLSFCQFRNAVMETRTDIDLEQWFLNQPGVNPSTISSWNDFAPRLGTPG
                     YPVSFVRHFIKWFVYPKSIKHPVNSLFEMIEQDEAP"
     gene            5996..6136
                     /locus_tag="PKM72_10115"
     CDS             5996..6136
                     /locus_tag="PKM72_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61186.1"
                     /translation="MRKAWQGWMMCKVWLLSPRLTVWGEGMMEGLIFSLIALGMWRVT
                     LD"
     gene            6210..6653
                     /locus_tag="PKM72_10120"
     CDS             6210..6653
                     /locus_tag="PKM72_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP61187.1"
                     /translation="MRRVDYIVGARDFKGLIAEQFMQDAVYAYHPEDSAEALAQTMTE
                     EGFGSVPIVDHDQHLLGIVSEFDLLHAIKEERSLLGVPASDLMTPAPVTVKRETPAMD
                     IIDRLEGKHLIRMPVVDEEGKLLGVVARRDILLGYLNATRQTKVF"
     gene            complement(6676..>8382)
                     /locus_tag="PKM72_10125"
     CDS             complement(6676..>8382)
                     /locus_tag="PKM72_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP61188.1"
                     /translation="TQAAFHQLAEEKDVVDFQNRIRDIRGNYLWLEWRATPQLQEKAI
                     YAVARDITQRYHATKALRRYEQIVSTSSDFLCFLDEENCLQAVNHGFCEYFNLPAREI
                     TGRHIRDFVGADLFETQLTSHLAECRKGRVVCFHQHLTFPEGTIRFMEVRLNPYVEPD
                     GIISGVTAIWRDLTEQKRLEQHVRQTQKMESIGTLAGGIAHDFNNILMAILGWAEMGT
                     WKHPQGDPAYKYFEQIGIAGKRGRDLIQQILTFSRQTEHQPRPVDLGPVIEESLTLLR
                     ATFPSNIDLRVVIHQKMNMVFADPVQIHQIIMNLCSNAEQALRPNGGTLTINLLAIII
                     EKDDFRFPPTLNPGRYVQIVVSDSGPGIPDHILPKIFDPFFTTKSVGKGTGMGLAVVH
                     GIVENLRGHISVQSSNKEGTTFLIALPEYQIPTDSSFDSKPSPIFTGSGHVLMVDDEV
                     SITEVLKEFLEHLGYTVTVRINPLEALEVFRMNPHQFDCLITDQTMPTLGGDLLSREI
                     LKIRPDMPIILCTGFSHTIDMEKALEQGISAFLYKPILLDQLSQTLTRVLSHSKPEIP
                     GS"
BASE COUNT         2071 a         1903 c         2178 g         2130 t
ORIGIN      
        1 cggcaactgt atattttgaa atcccggggt atggcgcatt cgcatcaggt tcggggatat
       61 acgatgaatc atcgtggctg gcaggtaaaa gaagtgtctg gaggtaaccg ttaggaccat
      121 gaaccggctc gattcaacat acccatttga acctatgaag tcgggggact gtgtctctag
      181 cggcaaggga gaaatggata gaatgcttgc aaggattgtt tatgcctcaa agcatccacg
      241 aatgattagg tgatcgagga gggtctcatt gctatgaaaa atgcgtctaa aacctcatcg
      301 aggcccaagc ggggttctgc caagtcttct ccttaccact taaccctcta tgtcgccgga
      361 cattccccca aatcacgtct ggccatgagt aatctacaac aattgtgcga tacgcattta
      421 tcctcgaaat atacggtgga aatcgtagat cttctccagg agcctaagag agccaggctt
      481 gatcagattt tggccattcc caccctggtc aaaaaattgc ctcctccctt aaaaaaaatc
      541 attggagatt tatcgaatgt tgaaaaagtt ttaatggggc tggatctgat cgacgagtcg
      601 ggtgaggtgc ttatgtcaag ttcaatgaaa gtctcatcat gaaatcccat gcgccaacca
      661 cccccaggaa agtaccccct ggcgggaaac catcggggat gcgccttcga ttgtatgtga
      721 ccggggcgac gagtctctcg ctccgtgcca tggccaatat caaagtcatt tgccgtgaac
      781 acctcgacga acagaattct gaccttcaaa ttattgacct gtatcgcaat cccgctgccg
      841 cccgtgccaa tcatattttt gcggctccca ccctcatcaa agagtttcct cttccctgtt
      901 gcagaattat cggagatctt tcgaaaaaag accgtgtcct gcatgctttg ggattgaccc
      961 ctcctcttcc attgccttct tctggataaa gtttgggaaa tagccatggc cccaaagcat
     1021 aaaccgtctc tcgcaaagtc tcgtctttca agccgtgagg aaatattaca gctacggttg
     1081 aaagaagcgg aagacacgct tcaggccatt cgggaaggag ccgtcgatgc cctcatcgtg
     1141 gaaacaggtg agggagatca agtctttacg ctcaagagtg cggattatcc ctatcgactc
     1201 atcatcgatc aaatgatgca gggcgcggtg atttttgatg agagcggtat tatattttat
     1261 gcgaatacca ccttcgccag catggtcaac cattcccacc aggaactatt tggttttcct
     1321 atcgaatcat ttattgctga ggacgatcga gaatttttcc taactctggc tggttctcat
     1381 tcaccggttt ccggaagaac aggaacgatt catttggtct cccaagacca ttatgatctt
     1441 gccgttttca tcggcatgac gtccattcac atcgataacc agcacttgca ttgtgcgatt
     1501 ctgaccgacc tgacggagca gaagaaatat gaccgggcgt tggcggaaga aaaattctcc
     1561 agaatgatct tggagcaaac aggagaagcg gtggttgtgt gtgatcggtc cggtcgaatc
     1621 atacgccaca gccgcgcgat cgatagactg atccagcaac ctatcctcca tacccggttt
     1681 catgaacatt ttcatttcac gttcatccag accccggttc cctcaggcgg tcgtgagcca
     1741 tggaatgaag aggggaatgt ggtccaagaa gtgctcggcg gcaggacact gcgtggtctt
     1801 gaaggcaatt tgcagatcgc gaacagaaac attcctgtgc tcctgagcgg cagtcctttg
     1861 caggacgacg aagtgaacac ggtaggagct attttcacga ttgtggatat tacggaaagg
     1921 aaaatggccg aacgggattt acaggtcttt aataattctc ttgaacaacg cattcaggct
     1981 cgcacacagg atttgttgag ttatcaaaaa catcttcggg agatgacatc agaattagtc
     2041 gtgaccgaac aacgcgaacg acgacgcctg gcaagtgaac tgcacgatta ccttgcccaa
     2101 ttgctggtcg tctgccggat gaaacttgcc caacagaacc gggaagcctg gagtccggaa
     2161 ttgaaaacgg atatcgagga aatcgatacc atcctgtccg attcactcac ttatacgagg
     2221 accctgattg cggagttgag tcccagtatt ctttatgaac tgggaattgt gcctgccctg
     2281 gtctggcttg gggggcaatt tgaacggcat ggtcttcagg tggacataca ggaagaagac
     2341 aaaaacattc aacttccgga agatcacgcg atatttgtgt ttcaggctgt cagggaactc
     2401 ctttttaata ttaaaaaaca tgccgacacc acacaggcca gggtttgtct caaaaagacc
     2461 gcatcccacg aattccaagt gaaggtggag gatacaggga aggggtttcc cctgtccgat
     2521 gcctcgcatg attattccag cccgggaaaa tttggactgt tcagcattcg tgaacgggta
     2581 gaggctttgg gcgggagtat tgatatcaaa gcggaagttg gacagggaac ttgcattacg
     2641 ctcgtggttc cgtttcattc tcccatttca tcagaggctt ctctcgatac caaggcaaag
     2701 tctgtgacat cgttggcatc tttcccctca aacaccccgc agaaaaaagg gactcgtatt
     2761 cttcttgttg atgatcatgc tcttgtcaga caaggcattc gcgggatatt agaggttcaa
     2821 gcagattttc aagtcgtggg ggaagcacaa gacggactgg aagccattga gtatgcacgc
     2881 acccttcatc ccgatctcat tgtcatggat gtgaatatgc caaagatgaa tggcattgag
     2941 gcgactcgca gaattattca ggaatttccc ttgatggcca ttatcgggct gtctgtccag
     3001 gaagataagc atgtccagga attactcctg aaggcaggcg cgggcatgtt tctcaccaaa
     3061 aatggaattg ccagtgaact cgttggcagc attcgtcaga tggttgccca cactccatag
     3121 ccctccaacc ttcagctcgt tggtggcaga accgcttttg aagttcattc cagcgtcgtg
     3181 tgaactcttc ctttctgcat tctcaggtgg gacctcttgt tatcgggtca cgtgtgtcag
     3241 agcacaaccc atcagttctg gcggatttat cccgcttgcg ctgcttgggt cccctcaata
     3301 gtacggagcc agcgggagct atgttgagga acgagtggct ggcggaatgg acattcccga
     3361 acgcgcctca agaataatct gatgaatata ctggagcttt gcgataacgg gaggaaccag
     3421 tacctccggc atcagatcaa ttaagcccat ggctcgattc atttcgttca ttcccagccc
     3481 aagccgttgg taggatcgaa tcatgtctcc gatgtcggct ttcgtcggat cgattccctc
     3541 tccgattccc gtatgtctcg cggtatcgag cacactcacg atcgcaaggt atttggcaaa
     3601 tgtctccgca aaatcctccc atgggtgcat ggtggcatag acactgatgt agttggcttg
     3661 ccagtcagat ggcgggcctt cccgatagtg ccgttcaagg gcttgcgcat aggttggctg
     3721 ttgataattg ccgaagatcc ggttgaacgt ttcttcctgc ttgtcctgta tcaacatttc
     3781 ccaataatag tgggcaatct catgtcggaa gtgaccaatg atggtccggt gagcttcccc
     3841 aaatgagacg cgtaattttt cccgctcaac atcttcggcc tcacgcagat tgatggtgat
     3901 tttcccgttc tgatgtcccg tgaacacctg ttcatggtct cccatggtat gccagtgatt
     3961 cccggagagg ctgacatcgg ctttgaaatc aaaggataag gcgggttgaa tgccgtcttc
     4021 tgctttccca tagggaagac ccaataggtc caaggtgtaa agtaagcgtc gtttgccggt
     4081 ttccagacga taccaacgct ctcgatttcc ctcaacagtc agatcgggaa tcgtgtcatt
     4141 atagcggcag tagtcacaga gctgcgtgac gagttggtca ttggggcttg ctcgaagaca
     4201 tcgattacag acctgttcga caacataatt atgacatttc agtagtgggg catggcatgc
     4261 cgtgtagcca catcgatagg cgccgtcagg actcacgagt agccccgaca tacgatggca
     4321 agccgggcac catcccactt ccgaattgca ggataggcag tggatatttt caaaaaacaa
     4381 cgtattgccg caatggcagg taaacatttt catggtcatt tattcccggt taagtgtgaa
     4441 tgtgagaatc caggcgaggg gaagaaagac ccttcatccg gctgtacaaa cagccatcat
     4501 catgaatcag aaaatagaca cgccggtttt ggtggtgaat aattcgagtg catattgtcc
     4561 cgccaatgaa ttgccctttt catttaatcc gggagaccac acggctacag tgagnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgttg ataaatggat ttctgggtat
     4741 gccatgttca tattcgagtt ggacgagaga attgaaggtc gatccggatg gttcgagtcc
     4801 aacccgtgtc caaatgttgt cgccttcaaa tggaagtccg agggtcagtg tatacacctt
     4861 tgaaatgctt tgaatcgaga agggctctaa ggcctgcccc acctggaaca catccccgtc
     4921 aacggtctgg accgccattc caaaattctg aggggaaacc gatgccaatt ggggaatgta
     4981 ggtggcaacg gttccgcacg gtaacaggtt taggacttct tgatggattt cattcaaaat
     5041 ggcttgatag ttcatacagg ttcacatctt ttacaggcag gtatgtatgt attggtgttg
     5101 ggtgagggcc catgacaaca ggaagggagc ggacttaagg ggcttcatcc tgttcaatca
     5161 tttcaaaaag gctgttcacc gggtgcttaa tggatttggg atagacgaac catttgatga
     5221 aatgtcgtac aaaggagaca gggtagcctg gggtgcctag cctgggagca aagtcattcc
     5281 agctcgaaat agttgaggga ttcactcccg gctgattgag aaaccactgt tccaggtcta
     5341 tgtccgtacg agtttccatg acggcatttc gaaattgaca aaaagacagg ccgaaatgcc
     5401 gaagaaaata gccgtcaatg ccgatcggac tgcccaagga tatgcgataa agaagcggga
     5461 agttccgctg ttggcaagcc cgcactttat cggtcatacg ggcgagccaa cagcaacttg
     5521 ctaaggtgtc ttgtgggcga cgaagtttca tatttatgtg cgtaaaagaa cccgtacgaa
     5581 atcttacctc attttagtcg aggaggccta tgctagtaga caatgtaatt cagggccctc
     5641 aagaatttta cgggaatgct gaagcacacg tcatgtgttt ttctaatagt gctgctcgtg
     5701 gtcttttttc gcacagcact gttcaccgtg cctgtgcccg ggttctctta aaaagaggcg
     5761 ctttactttt attcaaaaaa agcgcatggt agaaaaaatc tagaaaatcg aggtcgtcat
     5821 ggggtaaatc gttattcctt caccctatcg gaattgatcc ataagaaggc ctttcgggga
     5881 tgcgcatgcc cggaaggcct tcttgttttc gtcatgaaac acacataata gatccaaaga
     5941 tgaagctgaa attctcatag gtgagtgtcg tcgaggaggg gaaggaggtg aggccatgcg
     6001 aaaagcctgg caggggtgga tgatgtgtaa agtatggctg ttgagccccc gattgacagt
     6061 ttggggggaa gggatgatgg aaggtcttat tttcagcctc atagcgttgg gaatgtggag
     6121 agtgacgttg gattgaagga tctgatggtg ccctgtggtc tttcaacaca tgagttcacc
     6181 ccagttctct gaacaaggag ggatggatta tgcgacgcgt tgattacatc gttggagcca
     6241 gggactttaa aggtcttatt gccgagcaat ttatgcaaga tgccgtgtat gcctatcatc
     6301 ctgaggattc ggcagaagcc ttggcgcaga cgatgaccga ggagggtttc gggagcgtgc
     6361 cgatcgttga ccatgaccag catttgcttg gtatcgtcag tgagttcgat ctgctccatg
     6421 ccattaaaga ggagcgttct ttattaggag ttccggcgag cgacttgatg acacctgcac
     6481 cggttacggt gaagcgggaa acgccggcta tggatattat tgatcgactt gaagggaagc
     6541 atttgattcg aatgccggtg gtcgatgaag agggcaaact ccttggcgtc gtggccaggc
     6601 gggatatcct tctggggtat ttgaatgcca cccgtcagac aaaggttttt taggagagaa
     6661 atgctagagt ctcagtcatg atccgggaat ttcgggtttg gaatgcgaca gtacccgggt
     6721 gagcgtctgg cttagttgat ccagcaggat tggcttgtag aggaatgcgc taattccctg
     6781 ttccaaagcc ttttccatgt caatggtatg actgaagccc gtacaaagga tgatgggcat
     6841 atcggggcgt attttgagaa tttctcgaga cagcaggtct cctccaagtg tgggcatcgt
     6901 ttggtcggtg atgagacagt caaattggtg gggattcatg cgaaacactt caagggcctc
     6961 caaggggtta atgcggacag tgacggtata tcccagatgc tccaggaatt ctttcaagac
     7021 ttccgtaatc gacacttcat catccaccat cagcacatga ccacttccgg tgaagatggg
     7081 ggaaggcttt gaatcaaagg aggagtcagt gggtatctgg tactcgggca gggcgatgag
     7141 aaaggtggtt ccctccttat tggaggattg taccgaaatg tgtcctcgga gattttccac
     7201 aatgccatgt accactgcca atcccattcc ggttcctttt ccgacggatt tggtggtaaa
     7261 gaacgggtcg aaaatttttg gaaggatatg atcgggaatc ccagggccgg aatcactgac
     7321 gacgatctgc acataccttc cgggattcag tgtaggggga aatcggaagt cgtctttttc
     7381 aatgatgatg gcaagaaggt tgatggtcag agttccgcca ttggggcgaa gggcctgttc
     7441 ggcattagag cagaggttca tgatgatttg gtgaatttga acggggtcgg caaacaccat
     7501 attcattttt tgatgaataa ccacacgcag gtcaatgtta gaaggaaagg tggcacggag
     7561 cagagtcaga gattcttcaa taaccggacc cagatcgacc gggcgaggtt ggtgttctgt
     7621 ttgacggctg aaggtgagga tttgctggat gagatcccgt ccacgttttc ccgctattcc
     7681 gatttgctca aaatatttat atgcgggatc cccttgggga tgtttccagg ttcccatttc
     7741 cgcccatccg agaatcgcca tcagaatatt attgaagtcg tgtgcgattc ctccggccag
     7801 ggtgcctatg gattccatct tctgtgtttg tctcacatgc tgttccaggc gtttttgctc
     7861 ggtgagatcc ctccatatgg ccgtcacgcc tgaaataatt ccgtccggct ctacataggg
     7921 attcagtctg acttccataa agcgaatggt tccttctgga aatgtcaggt gctgatgaaa
     7981 acagacgacc cgcccttttc ggcattccgc taaatgactg gttagctgtg tttcaaacaa
     8041 atccgctccg acaaaatccc ggatgtgacg accggtgatt tcccgggcag gcagattgaa
     8101 atattcacaa aatccatgat tgacggcttg caggcagttt tcctcatcta aaaagcagag
     8161 aaaatcggaa ctggtcgaca ctatttgttc atatcggcgg agcgctttag ttgcgtgata
     8221 ccgctgggtg atatcccggg cgacggcata gatggctttt tcctgaagct ggggagtggc
     8281 acgccattcc agccagaggt aattgcctcg gatatcacgg atgcggttct ggaaatccac
     8341 cacatctttt tcttcagcga gttgatggaa cgctgcctgg gt
//
LOCUS       DAORAQ010000116         8379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1346_length_8379_cov_61.597790, whole genome shotgun sequence.
ACCESSION   DAORAQ010000116 DAORAQ010000000
VERSION     DAORAQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_8379_cov_61.597790"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKM72_10130"
     CDS             complement(<1..289)
                     /locus_tag="PKM72_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNP61189.1"
                     /translation="MPSLPPTPTFPPPDTTHPQLTIRAIATGMVLGVLLTPCNIYSGL
                     KIGWTFNMSITAALLSYAFWKIFETTAQTERWGLLENNINQTTASSAASIIS"
     gene            complement(347..1579)
                     /locus_tag="PKM72_10135"
     CDS             complement(347..1579)
                     /locus_tag="PKM72_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HNP61190.1"
                     /translation="MIESKIDTQYLLHILQKLLVIPSPTGYTDQIVHAVSGELKQLGI
                     PFELTRRGAIRATMAGQVSTPDRAIVAHLDTLGALVKWLKNNGRLAIVPIGTWSSRFA
                     EGARVTVFSDTKKHRGTILPLKASGHTYGDEIDTQPVSWKNLEIRLDETVTDANGHIQ
                     HLSNEEDLRRLGIHIGDFIAIDPSPEISPNGFINSRHLDDKAGVATILAAAKSILESG
                     VTLPVDCHLLFTISEEVGSGASAILHGDVAEMITIDNGTPAPFQASSEFGATIAMADS
                     AGPFDYHLVRRLIALCVEHDIPFQRDIFCDYRSDSASAVEAGNDIRTALLTFGVDSSH
                     GYERTHLSGLEAIAKLLVVYMKNEPVFWRDRDSLGSDNDFPTQPIEEHSFPQTLEDMV
                     RRHGEHQHDSLRSGTEDR"
     gene            complement(1604..3388)
                     /gene="ngg"
                     /locus_tag="PKM72_10140"
     CDS             complement(1604..3388)
                     /gene="ngg"
                     /locus_tag="PKM72_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HNP61191.1"
                     /translation="MASKDRDVLKAPQALHMTSPRQRPIGPTVKNWEVGPATYQGRRV
                     KPKVVLDCGWGRLIFAHTFTGPEEVASTLMQERPGHRDIAFYLRDPHVVLALRPHKLF
                     LDPSHTFRLWLADYQSVAHQKSGMVITPIQSEADVRALNRIYAARGMVTADTDILMRH
                     RRSQKLCYFLAKDQATNEVIGVILAVNHRLIFDDPEKGSSIWSLAVDPQAHHPGVGEA
                     LIRHAAERMKARGCNYVDLSVLHDNHEAIALYRKLGFGRIPVFAVKTRNTINEKLFAG
                     PSPDQRLNPYATIIVNEARRRSIDVDVLDAENSYFRLTFGGRSITCRESLSELTTAIA
                     MSRCADKTLTRRVLREAGLSVPAQMEANGRKHNGKFLEQHQSIVVKPAMGEQGAGVSV
                     DVRRVKEMESAITLAGKCGDPVILEQYVRGEDLRIVLINYEVVAAAVRRPPRITGTGH
                     HSIAELIDRQSQRRARLTGGESRIPLDGETRRCVKEGGFTLTEVLPKGKTLTVRKTAN
                     LHTGGTIHDVTGKLHPDLATAAVVGAKALAIPVVGFDFIVTSVSKPEYVIIEANERPG
                     LANHEPQPTAERFIDLLFPQTKTPTKLK"
     gene            complement(3378..5150)
                     /locus_tag="PKM72_10145"
     CDS             complement(3378..5150)
                     /locus_tag="PKM72_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HNP61192.1"
                     /translation="MCGILGQINFSDDPVDPQELMAMNATMTSRGPDGVGLYVQGRLG
                     FGHRRLKVIDLTQASQQPMVDSKLGLGIVFNGAIYNYKDLRKELETKGYTFFSHGDTE
                     VILKAYHAWGEDFIHRLNGMFAFAIWERDSERVVLARDRLGIKPLYFTETSQQFRFAS
                     TLPALLAGGNVSTEMDPVALHHYFMFHAVVPAPHTLLKSIRKLAPGTMMVIEPTGAQR
                     QQYYWQLSFEPREDERILKERDWQDRLYEVLRSAVRRRLIADVPVGVLLSGGLDSSLI
                     VGLLAAEGATGLNTFSIGFETVAEEKGDEFSYSDIIANEFSTRHHKLPVDTTHVLPNL
                     PGCIHAMSEPMVSHDAIGFYLLSKEVSKHVTVVQSGQGADEIFAGYHWYPPLLNSQHP
                     VDDYRQVFFDRDQKEFRRVIHPRFHEDDHSQRFVEKHFSQPGASRPIDKALRLDTTIM
                     LVDDPVKRVDNMTMAWSLEARVPFLDHEVVELAARVPAEMKIAQGGKGILKEVARRVI
                     PSEVIDRPKGYFPVPALKYLRGPYLDMVKDALVSETSQKREIIQRDYIDELLKNPDDH
                     LTPLRGSKLWQLGLLELWLQSHGI"
     gene            5499..6251
                     /locus_tag="PKM72_10150"
     CDS             5499..6251
                     /locus_tag="PKM72_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP61193.1"
                     /translation="MKNITRVTEIAPDHYSISIYVPEFNLRFNHFLIKDEEPLLFHTG
                     MKRMFPMIQEAVARVIDPTTLRWISFSHFEADECGALNEWLTIAPQAEPVCGLVGALV
                     SVNDFSHRQARMLAHDEILETGKYRLRFRQTPHVPHNWEAGLLFEEVTRTLLCSDLFS
                     HEGDVEAVTESDIVEPAKRALIEGQNSAFANAYPYTPLTGPILHGLADLQPKQLALMH
                     GSVFVGDGKQALHDLASVMREMLGKSAESFPQ"
     gene            6475..6879
                     /locus_tag="PKM72_10155"
     CDS             6475..6879
                     /locus_tag="PKM72_10155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNP61194.1"
                     /translation="MNITQIRIDPDPYEPYRLSQGYRVGDLLFISGQTAIDTSGQIVG
                     VGNFDVQGKQTFDNLERVLRAGGSSLRNVIKVTIFLTDMTHFDKIVELRGQRFTPPYP
                     ADTIVEVRSLYSPDAMIEIEAIAVADSAAERK"
     gene            6992..7441
                     /locus_tag="PKM72_10160"
     CDS             6992..7441
                     /locus_tag="PKM72_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNP61195.1"
                     /translation="MSEHKITVEWKRASEDFSYESYNRDHILVFEGGVRVPASAVPAY
                     GGNPAHVNPEEAFVAALSSCHMLTFLAMAVKKRFVVDCYIDHAVGLLEKNQNKKLAIT
                     RAILRPQVMFGGPTLPTREQIHALHERAHSECFIANSVTTEVTVEAE"
     gene            7552..>8379
                     /locus_tag="PKM72_10165"
     CDS             7552..>8379
                     /locus_tag="PKM72_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNP61196.1"
                     /translation="MSERSPKQVREVVEEVYRTESRRVLATLIRLLGDFDAAEEALHE
                     AFAVAVDQWTRDGVPANPRTWLVSTGRFKAIDGIRRRTRFDASLAEFATQLEAGIHDP
                     QENTDDSIDDDRLRLIFTCCHPALSSEAQVAMTLREVCGLTTEEIAKAFLTSPPTVAQ
                     RIVRAKAKIRKARIPYEVPSETALPDRLDAVLRVIYLVFNEGYSASSGESLTRHDLSA
                     EAIRLGRLLLELLPDGEAMGLLALMLLHDSRRAARISRTGDLILLEDQDRSLWNRDQI
                     "
BASE COUNT         2012 a         2231 c         2159 g         1977 t
ORIGIN      
        1 acgagataat ggatgcagcc gaagaggccg tggtttgatt gatattattt tcgagcaaac
       61 cccagcgctc ggtttgggcg gtggtttcga atattttcca aaaggcatag ctgagcaagg
      121 cagcggtgat ggacatatta aatgtccacc caatcttcag tccgctatag atattgcagg
      181 gggtgaggag tactcccaag accattcccg tggcaatagc acgaatcgtc aactgtggat
      241 gggtcgtgtc gggtggggga aaagtaggtg tgggtggaag agagggcatt aagctgacag
      301 agtaatgagg agtcaacccg caagggggaa tgtgttcatc accccatcat cggtcttcag
      361 tccctgaacg caatgagtca tgttggtgtt caccatggcg tcgaaccatg tcctcaagag
      421 tttgaggaaa agaatgttct tcaatcggtt gagtggggaa atcattatcg gaccctaacg
      481 aatcgcgatc tcgccaaaac accggttcat ttttcatata cacgacgagt aacttcgcaa
      541 tggcctcgag cccactcaga tgagtccgct catagccgtg ggaggaatcg accccgaacg
      601 taaggagggc ggtccgaatg tcattcccgg cctctacggc cgacgcactg tcacttctgt
      661 agtcacaaaa aatgtctcgt tgaaacggaa tgtcatgctc gacacataag gcgatgagcc
      721 gcctgaccag gtgataatca aaaggcccgg ctgaatcggc catggcaatc gtcgcgccga
      781 attcgctcga ggcttgaaag ggcgcggggg tgccattatc aatcgtgatc atctctgcca
      841 catcaccatg taagatcgct gatgccccgg atccgacctc ttcggaaatc gtaaacagta
      901 aatggcaatc cacggggagc gtgaccccgc tttcaaggat ggacttggca gccgccagga
      961 tggtggccac tccggcttta tcatccaaat gccgcgagtt aataaatcca ttgggactga
     1021 tttccggtga gggatcaatg gcaataaaat caccaatatg aatcccgagt cgtcggagat
     1081 cctcttcgtt cgataagtgt tggatatgtc cattggcgtc cgtaaccgtt tcatcaagtc
     1141 gaatttccag attcttccag gacaccggtt gggtatcgat ttcatcacca taggtgtgcc
     1201 cggatgcttt cagaggcagg atggtgccac gatgcttctt tgtatcggaa aaaacggtca
     1261 cgcgcgcacc ctcggcaaac cgacttgacc aggttccgat cggcacaatg gccagccggc
     1321 cgttgttttt gagccattta accaaagccc ccagggtatc cagatgagcc acaatggctc
     1381 gatcaggtgt actgacctgt cccgccattg tggctcgaat cgctccgcgc cgggtgagtt
     1441 cgaagggaat ccctaattgc ttcagttcac ccgataccgc atggacaatt tgatccgtat
     1501 agccagtcgg gctcggaatc accaacaatt tttggagaat atgcaggaga tactgcgtgt
     1561 caattttcga ttcgatcata caaaccccca tcaccccaaa ggactacttc aattttgtgg
     1621 gtgtcttcgt ttgaggaaac aacaggtcga taaatcgttc tgccgtcggt tgcggttcat
     1681 gattggcgag accgggtcgc tcgtttgctt caataatgac atactcaggc ttggaaaccg
     1741 aggtgacaat aaaatcaaat cctaccaccg ggatagccag cgctttggca ccaaccaccg
     1801 ccgcagtggc taaatcagga tgaagctttc ctgtcacgtc atgaatggtt cctccggtat
     1861 ggagattggc cgtcttacgc accgtgaggg ttttgccttt tggcaaaacc tctgtaaggg
     1921 taaaaccgcc ctctttcaca cacctcctgg tttctccgtc cagaggaatt cgactctctc
     1981 cgcccgtcaa tcgggcccgt cgctgactct gccggtcaat caattcggca atcgaatgat
     2041 gacctgtccc cgtgattcgg ggtggccgtc gaacggcagc ggcaaccact tcatagttga
     2101 tcaacacaat gcgtaaatcc tcgcctctca catattgttc cagaatgacg ggatcaccac
     2161 atttaccggc aagggtgatt gcggactcca tttccttgac cctccgcaca tccacactca
     2221 ccccggctcc ctgttccccc atcgccggct tgaccacaat ggattgatgc tgttcaagaa
     2281 atttgccgtt gtgcttccgc ccattggctt ccatctgagc tggaacggat aggccggctt
     2341 cccgcaacac ccgccgcgtg agggtcttat ccgcacaacg gctcatcgct atcgccgtgg
     2401 tcaattcgga caacgattcc cgacaggtaa tggaccggcc cccaaacgtc aagcggaagt
     2461 agctattctc ggcatccagg acatcgacgt cgatgcttcg gcgcctggct tcatttacaa
     2521 taatggtggc ataggggttg agtcgttgat ccggtgaggg acctgcaaac aatttttcat
     2581 ttatggtatt tcgcgttttc accgcaaata caggaattcg tccaaagccc agtttccggt
     2641 agagcgcaat ggcctcatgg ttgtcatgca gcactgagag atccacatag ttgcaccccc
     2701 gggccttcat ccgttcggcc gcatgacgaa tcaacgcttc ccccactccg ggatggtggg
     2761 cttgagggtc aacagccaga ctccagatgc tactgccttt ttcggggtcg tcaaaaatca
     2821 ggcgatgatt cacagctagg ataacgccaa tcacttcatt cgtggcctgg tctttagcga
     2881 gaaaatagca gagcttttgg gagcggcgat gcctcataag gatatcggtg tcggccgtaa
     2941 ccatgccccg tgccgcatag atccgattca acgccctgac atcggcctcc gactggatgg
     3001 gagtgatcac catgcctgat ttctgatgtg cgaccgattg ataatcagcc aaccacaatc
     3061 gaaacgtatg cgaaggatcc agaaacagct tatgcgggcg cagggccaga accacatgag
     3121 gatcccgtag ataaaaggca atatccctgt ggccgggacg ttcctgcatc agcgtggaag
     3181 ccacctcttc ggggcctgta aaggtgtggg caaaaatgag ccgcccccag ccgcaatcca
     3241 agaccacttt gggcttcact cgccgaccct ggtaggtcgc cggcccaact tcccagtttt
     3301 tgaccgtcgg tccgatcggt cgctggcggg ggctggtcat gtggagagct tgcggggcct
     3361 tcaacacatc gcgatcctta gatgccatgg gactgaagcc acagttccaa taatccaagc
     3421 tgccagagct ttgacccacg taatggtgtg agatgatcat ccggattttt taacaattca
     3481 tcgatatagt cccgctgaat aatctcgcgt ttctgactgg tctctgacac aagcgcgtct
     3541 ttcaccatat ccaaataagg gccacgaaga tatttcaagg cgggaacggg gaaatacccc
     3601 ttgggacgat caatgacctc ggaagggatc acccgacgag cgacttcctt cagaatgcct
     3661 tttccccctt gcgcaatttt catttctgcc ggcacgcgtg cggccagttc gacgacttca
     3721 tgatctaaaa acggcactcg cgcctccaaa ctccatgcca tcgtcatgtt gtcgacgcgc
     3781 ttgaccggat cgtcgactag cataatggtc gtatcaagcc ggagagcctt atcaatcggt
     3841 cgggacgctc ccggttggga aaaatgcttc tccacaaaac gctgactgtg atcgtcttcg
     3901 tgaaaccgtg gatgaatgac tcgacgaaat tctttctggt cacgatcaaa aaacacctgg
     3961 cgatagtcat caacgggatg ttgactgttc aagagggggg gataccagtg atatcctgcg
     4021 aaaatttcgt cggcgccctg accactttgc accacggtga catgcttgga cacttcttta
     4081 gaaagcaaat aaaacccgat ggcatcgtga ctgaccattg gctccgacat agcatgaatg
     4141 caacctggca gattgggcag aacgtgggtc gtatccacgg gaagtttgtg gtgccgggtc
     4201 gaaaattcgt tggcaatgat atcggagtag gagaattcgt cccccttttc ctctgccacc
     4261 gtttcaaacc caatggaaaa cgtattcaga cccgtggcgc cttcagccgc taacaggccc
     4321 acgatgaggc tcgagtccaa cccaccggac agaaggaccc ccacgggcac atccgcaata
     4381 agccgtcggc ggacagccga ccgtaggacc tcatagagcc ggtcctgcca gtctcgttcc
     4441 ttcaagatcc tctcatcttc acgcggctcg aaggagagtt gccaatagta ctgttggcgt
     4501 tgtgctcccg ttggctcaat gaccatcatg gtgccgggcg ctaattttcg aatgcttttc
     4561 agcaaggtgt gtggtgcagg caccacggcg tgaaacatga aatagtgatg aagggccacg
     4621 ggatccatct ccgtggagac gtttcccccc gctaagagag ccggcaaggt cgatgcgaat
     4681 cgaaactgct gagacgtttc agtgaaataa agcggtttaa ttcccaaccg atcacgcgcc
     4741 agcacaaccc gctctgaatc tctttcccaa atggcaaacg caaacatgcc gttcaagcgg
     4801 tgaataaagt cttctcccca cgcatggtag gccttgagaa tgacttcggt atctccatgc
     4861 gaaaagaagg tatagccttt tgtctccagc tcttttctta aatccttgta gttgtaaatc
     4921 gcaccattga agacaatacc aagtcccagt tttgaatcca ccatgggttg ttgggaagcc
     4981 tgggtcaaat caatcacctt gagccgtcgg tgcccgaacc ctaaccgccc ttgaacgtac
     5041 agcccgacgc catcaggacc acggctggtc atagtggcat tcatagccat caactcctgg
     5101 ggatcgacgg ggtcatctga aaaattgatc tggcctagaa ttccgcacat atgttcatct
     5161 tcctttgatt atccgaaaat tcccttaccg tatatgtgat cgttgatgat cccataaaaa
     5221 acccaagcat ccggagctca tcccctccga agggcatgcc ctcaccaagg atagtccttt
     5281 taggaaataa cccattgtaa acgggaattg atgtctagtg gacggggaaa tccaccctaa
     5341 taattattct cgactttgtc aaacaaatct gatggaaagg ctctcatcaa atgacagaaa
     5401 atccgctgtg cgaaggtgcc ttcggcatga tatttttact catttaagga ggcaccgccg
     5461 ggggacgttt tcaacaccct gctggaacag gagttacaat gaaaaatata accagagtga
     5521 cagagattgc tcccgaccac tattcaattt ctatctatgt tcccgaattc aatctgcggt
     5581 ttaaccactt tctgatcaag gacgaggagc cgctgctgtt tcacaccggg atgaagcgga
     5641 tgtttccgat gatacaggag gcagtcgcac gcgtcattga tccgaccacg ctacgatgga
     5701 tttcattcag tcatttcgag gcggatgaat gtggggcgct caacgaatgg ctgaccatcg
     5761 ctccacaggc ggaaccggtg tgcggcctgg tcggggcact cgtcagtgtg aacgactttt
     5821 ctcatcgtca ggcccgcatg ctcgcgcatg acgaaattct ggaaaccgga aaatatcgac
     5881 tgcggttccg ccagactccg catgtcccgc ataattggga agcaggcctt ttattcgagg
     5941 aggtcacccg taccttactg tgctcggacc tgttctctca tgagggagat gtggaagcgg
     6001 tgaccgaatc ggatatcgtc gaacctgcca aacgggcgtt gatcgaaggg cagaacagcg
     6061 cgtttgccaa tgcctacccc tacactccct tgactgggcc aatactgcat ggcctggcag
     6121 atctccagcc aaagcagttg gcgctcatgc atgggtccgt tttcgtggga gatggaaaac
     6181 aagcgcttca cgacctggcg tccgtcatgc gcgaaatgct ggggaagagc gcagaaagtt
     6241 tccctcaata aacacctaca gacgtctcta ccgtcaagtg tcgaagggta gccatgttga
     6301 ttttaagaca cgggtcttgg ccgaagccgt gattcaattg gactctccga taccaacagg
     6361 aattaatgtc atccgaatgg atatactgaa caatcttcat gtgtcattaa ggagcaaatg
     6421 gtaagccaag tcgttttttg agatctgttt ttccattaaa aaggagaggg tcctatgaac
     6481 atcacacaaa tccggatcga ccccgatccc tacgaaccct atcgtctttc acagggttat
     6541 cgggtcgggg atctgctctt tatctcaggc cagactgcca tcgatacaag cggtcagatt
     6601 gtcggcgtag gcaactttga tgtccaggga aaacagacat ttgataatct tgagcgggta
     6661 ctccgtgcgg gagggtcgag cttacggaac gtcatcaaag tgacgatctt cctgaccgac
     6721 atgacccatt tcgataaaat cgtggaactc cgcggtcaac ggtttacgcc tccctatccc
     6781 gcagacacta tcgtggaagt ccgctccctt tactcccccg acgcgatgat tgagattgaa
     6841 gccattgccg ttgccgattc ggcggcagag cggaaataat agtcattcgg gtaaaataat
     6901 ctgagatgga atattttgag aataatgccg ccagtggcaa tcgtctggaa gtctgttggc
     6961 caaatatcac tttcccgaaa ggggttgaac gatgtctgag cataagatca cggtagaatg
     7021 gaagagggca tcggaagact tttcgtacga gtcttataat cgagaccata tcttggtatt
     7081 tgaaggtggt gttcgagtgc ctgcctcggc tgttcctgcc tatggcggga atcccgctca
     7141 tgtgaatcct gaggaagcct tcgtggcggc cttgtcgagc tgtcatatgc tgactttcct
     7201 ggcgatggca gtcaagaaac ggttcgttgt agattgctat attgatcacg ccgttggatt
     7261 attggaaaaa aatcagaaca aaaagcttgc cattacacgt gccattttac gtcctcaagt
     7321 gatgttcggt ggaccgactc ttccgactcg ggaacagata cacgccctcc atgagcgagc
     7381 ccatagcgaa tgtttcattg ccaattcagt gacgacagag gtcacggttg aagcagaata
     7441 accggcaact ccctctctta tcccccacca gcagaggcga acgctcgccg gcacacccgt
     7501 cgcttaccac agtggttttt ccgtacctct ccatgtgagg ccttttcgat catgagtgaa
     7561 agatccccca aacaggtgcg ggaggtggtg gaggaagtgt accgcaccga atcacgacgg
     7621 gttcttgcca ccttaatccg tctgctcggc gattttgatg cggctgaaga agcgttgcac
     7681 gaggcatttg cggtggcggt cgaccaatgg accagggatg gtgttcccgc caatccgcga
     7741 acctggcttg tgtcgaccgg tcgtttcaaa gcgatcgatg ggattcggcg acggaccagg
     7801 tttgacgcct cgcttgcgga attcgccacg cagttggaag ccggcattca tgaccctcaa
     7861 gagaatacgg atgacagtat cgacgacgac cggttacgtt tgatctttac ctgctgccat
     7921 ccggctcttt catccgaagc tcaagtggcg atgactctcc gggaggtttg tggcctgacg
     7981 acggaagaaa tcgcgaaagc ttttctcaca tccccgccca cggtggctca gcggatcgtt
     8041 cgcgctaaag ccaaaattcg caaggcacgc ataccctatg aggtaccttc ggagaccgct
     8101 ttgccggacc gtctggatgc ggtgctgcgg gtgatctacc tcgtgtttaa cgaaggatac
     8161 tctgcctctt caggggaatc tctcacacgt catgacctct cggctgaagc tattcgactg
     8221 ggacgtttac tgttggaatt actgccggac ggcgaagcga tggggctctt ggcattaatg
     8281 ctccttcatg actcacgccg cgccgcacgc atctctcgaa cgggagatct gattctgctt
     8341 gaggaccagg atcgttcgtt gtggaaccgg gatcagatc
//
LOCUS       DAORAQ010000117         8299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1359_length_8299_cov_74.081271, whole genome shotgun sequence.
ACCESSION   DAORAQ010000117 DAORAQ010000000
VERSION     DAORAQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1359_length_8299_cov_74.081271"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..305)
                     /locus_tag="PKM72_10170"
     CDS             complement(141..305)
                     /locus_tag="PKM72_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61197.1"
                     /translation="MDSAVIVLDLDEGKRMEKGDWSNDSRLKIAWLPTTVTIAIPKKV
                     MKSLLAVPNA"
     gene            346..696
                     /locus_tag="PKM72_10175"
     CDS             346..696
                     /locus_tag="PKM72_10175"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNP61198.1"
                     /translation="MARVELNDFHESRLIVDTGATYTTISENLAFDAGIRSDLANSRI
                     TLFTVGGKVQAELGIARKIRVGNTGRENVQVVIFTIPDLPDGIDGLLGLSFFDHFLVR
                     LDHSNQQLHLSPRT"
     gene            934..1377
                     /locus_tag="PKM72_10180"
     CDS             934..1377
                     /locus_tag="PKM72_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNP61199.1"
                     /translation="MRFFIYADNLNPTQLKRRAPEAQFLFKAYVPDHTIKFGRWSNQW
                     RCGLATIAPSAGERVWGGVFELTPEDVQEMDKFEGDLPEGAFRHVEVHVFTEDGEKEF
                     VSTHVAQSIGKFKAKDHYLDWILAGVKHWKLPDECVDMWDLFRSK"
     gene            1442..1768
                     /locus_tag="PKM72_10185"
     CDS             1442..1768
                     /locus_tag="PKM72_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNP61200.1"
                     /translation="MPKPKYHILVCTNSRPPGHPKPSCGAAGAQNLLMALNMGLIERG
                     VQPGEVLVTGTTCLGPCESGPNVVVYPTGTWYSQVKDSDVAVILDEHIKKGEPAAQLK
                     PDSVWA"
     gene            1794..2345
                     /locus_tag="PKM72_10190"
     CDS             1794..2345
                     /locus_tag="PKM72_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNP61201.1"
                     /translation="MRDHQHHEGQGSHTAAPSHLTHKIQEAPSLSCMVVTCSDTRTPE
                     SDTSGKLICHLLKEQGHSIAEYRIIKDEPTEIKALLLQAIGQEALQVIIINGGTGISR
                     RDSTFEAVDDFLEKRLDGFGEIFRFLSYQDIGSPAMLSRAVAGTHGKLVVFSIPGSQG
                     AVRLAMEQLILPEIGHITGELTK"
     gene            complement(2440..3372)
                     /locus_tag="PKM72_10195"
     CDS             complement(2440..3372)
                     /locus_tag="PKM72_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNP61202.1"
                     /translation="MDSPKNWLQFYQVDVFTSEPFGGNPLAVFPSAGELSEREFQQIA
                     REMNLSETVFVLPPTDPKAAAKVRIFTPLQEIPFAGHPILGTFYVLGTMKHLALQEPV
                     TKVFYECTLGLYALDLIVLKGQIEQVIMSQPSPQFIDVITQAEAIYDIAKALGIHRSL
                     IADTIFPVEVVSTGLPVLVVPIRSLTAAKSMEVDHSEVLEICARFGANGIMIFTTMTV
                     EESATVHTRMFADPIGISEDPATGSASGALGAYLVKNGVVEVGPTTEIVMEQGYEIDR
                     PSRILVQVFSDDDVIKEIKVGGQVVMVAEGKMVY"
     gene            complement(3771..5006)
                     /locus_tag="PKM72_10200"
     CDS             complement(3771..5006)
                     /locus_tag="PKM72_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61203.1"
                     /translation="MRELPILEAPKLFAGHDYRDVLKREMDAGKIPLSLGKECPVKCA
                     FCYELDHSYRETLDPPKTSQEDWQFILNYISSKPTDPLQFWCLGGNEYMEWTDLFLHP
                     KAMEWIEDFLKFTDKNIQFFTVGFVHVPKIHRLVDQYPGRINFELSVITLGEYRQKLM
                     PHAPSVKHLMKVLDGPAVSSANFYAFDKNTMSTDAETISRINSRCVLWMGCLTPVGGI
                     KEETARVMRQGRKYLAVEAEKIYDADLPNFTTIHTEAYVTAFLNRKRIVSLFDSLELE
                     KRDSIVMAGSVYRILAMFRKNRARFLSVPNATLGGDSDCTVLLTLNDIAKRLTKEKFI
                     YVPQCIVESGRGPGRDIAGVHIDDFVSKTGVKIRILPKISTKFANNRLYRNGSLQNYV
                     EDYVRNPLVRSYEAIAPIV"
     gene            5168..5431
                     /locus_tag="PKM72_10205"
     CDS             5168..5431
                     /locus_tag="PKM72_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61204.1"
                     /translation="MTLEYALLLFNLSEPFSHDVLQQRYEEARRIWFPSRYAGLTNNP
                     AKYMEMYKKGETKTKEIHAAYQILSDHLRNSGSSPQKYDHGAE"
     gene            complement(5416..7383)
                     /locus_tag="PKM72_10210"
     CDS             complement(5416..7383)
                     /locus_tag="PKM72_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP61205.1"
                     /translation="MTESLIEIERGGSTDKPRRIFKTMLLFPPEWVPTAPYLALPSLT
                     AVLRSYGHEVIQKDVNIEMYDWFFSDTFLIWVKLRMDQQRRGLNEREARNELTDFERD
                     RLACLASQDAIDVMELAERAIEAKVIVRGEAFYDAEKLEWALKTFRDVMQFISAAYFP
                     ASLVFYPMESNLGYRSGVSTEVLACLDDDLVNVYRDVCRQLVLPAIQKEKPEVIGVSV
                     GTQMQLLAGMTFCKMIKEEFPDIHVTVGGNVITRLKDDLPKRKQFFTQVFDSAILYEG
                     EHALVWLLEALAGERDWEKVPNLMWHQGGAIRVNEEIYTEKTTALPLPDFDGLPLDAY
                     FVPVRILPYLATRGCYWGRCTFCDHGQGYFDQYRGKSAHDVVREIKALKEKYRAEHFL
                     FSDESYPPALLKKVSQLLIEEQVGIKWTTLIRFEESLQDPEIWKQAVQAGCCTLYYGM
                     ESANERVLELMDKHAKKAVIENNLREAAKAGIWNHVMAFYGFPGETRDEAEETREFLL
                     DNKRYIHSVELFYFVAYRHTPMVRNPDKFGITIHKQEEYDMPLDYYYTLKEPGGLTCL
                     EAMQLAEEFYQKDFEPWAVRVNAREHVFLYISKFGTNYLPQIYAMKAGQDEPRTDGVQ
                     GLITWPVSHAPSEKNSKGEPGMSRVVSHSAP"
     gene            complement(7403..>8299)
                     /locus_tag="PKM72_10215"
     CDS             complement(7403..>8299)
                     /locus_tag="PKM72_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP61206.1"
                     /translation="DFDGLPLEKYFVPEPILPYLATRGCYWGRCEFCDHGEGYTAGYR
                     TKRDWQIIEELTYLKNKYHARSFHFTDESYPPALFRKLTKKLIESKLDIAWTTHIRFE
                     KSLLEDQVWADAQTSGCKFLHMGYESGSERVLQLMDKATTTEVIQRSLELSSQHGVWN
                     HVMGFFGFPGETYQDAKFSIQFLEDNREHVHSIGFGTFDLSKHTPVAKNPEKFGITYY
                     KNPEWDLALDYYFTVKEGLSVEDAERVFEEFEQNHYAGWDLKIFIREYVFLYVTHFGT
                     NKLPSLQFRLDSHDSSSKLASV"
BASE COUNT         2353 a         2066 c         1659 g         2221 t
ORIGIN      
        1 ctacaccact ccatcaagaa aaactagaac catttcactg ttcactccag tctttaaggc
       61 tatccccggt ccaaccgaca aacgttctga ggtccataca aaggaaagat tcattaacac
      121 atgctccttc aacaagggat ttaagcattg gggactgcca acaaactttt catcaccttt
      181 ttgggaatag cgatcgtgac agtggtgggt agccaggcaa ttttcagtcg actatcatta
      241 gaccaatccc ccttctccat tcgcttacct tcatccaaat caagcacaat aactgctgaa
      301 tccaatgatt ctgtgattcc ccttgaacat ttaggaggtg tttgggtggc acgagtggaa
      361 ttaaatgact tccatgaatc cagactcatt gttgatacgg gggcaacata tacgaccatt
      421 tctgaaaatt tggcatttga tgcaggaatt cgctcagatt tggcaaattc gcggattacc
      481 ctctttactg ttgggggaaa agtacaggcc gagttaggca tcgcacgaaa aattcgtgtg
      541 ggaaatacag gaagggaaaa tgtccaggta gtcatattta ccatcccgga cctcccagac
      601 gggattgatg gcctactggg tctcagtttt tttgatcatt ttcttgttcg tctggaccat
      661 tccaaccaac agttacatct ctcgccaagg acttaggcct aagagcccgg tccatctcta
      721 ccttgatgtc cattttccat caatcttgac ctaagtccca tccccaatcc ttggtttgag
      781 agttttatgg ccaggtcata agctctgctt cagatccttc ccgaccatac aaaatacagt
      841 taccgccctt aggacatcga aaatcagttc ttgacagttc gcgaaggcag tatggtaata
      901 ctacctttcc cctaaacaga caggacttaa cccatgcgct ttttcattta tgcagacaat
      961 ttgaatccca cgcagcttaa acgacgtgct cctgaagcac aatttttatt taaggcatac
     1021 gtgccagacc acaccatcaa atttgggcgc tggtctaacc aatggcgctg tggattggcc
     1081 actattgccc cttctgctgg tgaacgagtg tggggagggg tttttgagct taccccggag
     1141 gatgtccagg aaatggacaa gttcgaggga gatcttcccg aaggagcttt tcgtcatgtt
     1201 gaagtacatg tgttcactga ggatggagaa aaggaattcg tcagcacaca tgtagcccaa
     1261 agcattggaa aatttaaggc caaagatcat tatttagatt ggattctcgc gggagtcaag
     1321 cattggaagt tgccggatga atgcgtggac atgtgggatt tgtttcgctc aaaatgagcc
     1381 ttttatccat ttatcattga atttccattc acctttattt tttcattaag aaggagtttc
     1441 tatgccaaaa ccgaagtatc atattctagt gtgcacgaac tcccgtcctc ctgggcatcc
     1501 caagccttca tgtggagctg cgggagctca aaatttatta atggcactca atatgggctt
     1561 gattgaacgt ggggttcaac ctggcgaggt attagtcact gggacaactt gcctcgggcc
     1621 ttgcgaaagt ggcccaaacg tggtcgtcta tccgacagga acttggtact cccaggtcaa
     1681 agactccgat gtagcggtta ttttagatga gcacatcaaa aaaggagagc ctgctgcaca
     1741 gctcaaaccg gattccgttt gggcctaagc caggtttagc caccttggga accatgcgtg
     1801 accaccaaca tcatgaaggg caaggatccc ataccgcagc cccgtctcac ctcacacaca
     1861 aaatacagga agcaccctct ttatcctgca tggtggtcac ctgtagtgat acccgaacgc
     1921 cagaatctga taccagtgga aagttaatct gtcatctttt aaaagagcaa ggacacagca
     1981 ttgcagaata ccggatcatt aaagatgaac cgaccgaaat taaggccctc cttctccaag
     2041 ccattggtca agaggccctt caggttatca ttatcaatgg cgggacaggt atctcacggc
     2101 gagactccac atttgaagcc gttgatgact ttttagaaaa acgacttgac ggatttgggg
     2161 agatttttcg gtttttgagt tatcaagata ttggatcccc cgccatgtta agtcgggccg
     2221 tggcggggac acatggaaag cttgtcgttt tttctattcc aggttctcaa ggggcggtcc
     2281 gcctagcaat ggagcaactt attctacctg aaatagggca cataactgga gagctaacga
     2341 agtaggcaaa tctaggcatc ttcctgaaaa ttcagttttt atcctcatta tggaacattc
     2401 agaacacctc taacgcgagc gggggaggaa ttttacacgc tagtaaacca tctttccttc
     2461 cgccaccatt accacctgcc ctcccacttt aatttcctta atcacatcat catcagaaaa
     2521 aacctgcacc aaaatacggg atggccggtc aatttcatac ccctgctcca taactatttc
     2581 ggtggttgga ccaacttcca cgactccatt cttgactaaa taggcaccaa gggcgccact
     2641 tgcgctgccg gttgcaggat cttctgaaat acctattgga tcggcgaaca ttctggtgtg
     2701 aacagtggca ctttcctcca ctgtcatcgt cgtgaagatc ataattccat tggccccaaa
     2761 gcgagcacaa atctctagaa cttcagaatg atcaacctcc atggattttg cggccgtcaa
     2821 gcttcgtata ggcacaacga gcaccggcaa tcctgtcgag accacttcaa cagggaaaat
     2881 tgtgtcggca atgagcgatc tgtgaattcc caaagccttg gcaatatcat aaatggcttc
     2941 cgcctgtgtg atgacatcga taaattgcgg agagggttgc gacatgatga cctgttcaat
     3001 ttgccccttc agaacaatga ggtcaagcgc atacaatcct agggtacatt catagaaaac
     3061 tttagtcaca ggctcctgca aggccaaatg tttcattgtt ccgagaacat agaatgtccc
     3121 caaaatagga tggcccgcaa acggaatttc ctgaagagga gtaaatattc tgaccttagc
     3181 ggcagctttg ggatcggtcg gcggtaggac aaacaccgtt tcagacaaat tcatttcacg
     3241 cgcaatttgt tgaaactccc gttcactaag ctctcccgct gagggaaaca ccgctaaagg
     3301 attcccgcca aacggctcac ttgtgaatac atcaacctga taaaattgca gccagttttt
     3361 aggagaatcc ataatcaact agagaaacat ttaaggacgg attagaaaca tgtcacacga
     3421 atttcaaatc cacaaaacct tacatggtat tacatcgtac ccgaacactt ttactgccct
     3481 cccgtgacca tgcattcttc atacactcgt tgccagtcag gaacgcccct accccctaac
     3541 ctcgcattag tcatttcttc acatctgaaa gaatcatttt catttttctg cagattaaac
     3601 gtctcttggc tttccagatt tccggaacac cctatagccc caagtagcaa caaaaaaaag
     3661 aaaaatcctc ttagtctttt caccggcata atctcagaaa tttttatttg tcgggtagct
     3721 ttggaccacc taccctccca tcaaattcct ttacaaaact ttaaggcaat ttacactatg
     3781 ggcgcgatcg cttcatacga acgaaccaag ggattacgca cataatcctc cacatagttt
     3841 tgcaatgatc cattgcgata caatcgatta ttggcaaact tagtactaat ttttgggaga
     3901 atccttattt tcactccggt cttacttaca aagtcgtcaa tgtgaacccc cgcaatatcg
     3961 cgacctggac ctcttcctga ctccacaata cattgaggaa catagataaa tttttcctta
     4021 gtcaatcgtt tggctatgtc attcagggtc agcaggaccg tacaatcaga atctcctcca
     4081 agcgtggcat tcggaacaga gaggaacctg gctcgatttt tcctgaacat ggctaaaatc
     4141 cgatacacac tacccgccat gacaattgaa tcacgctttt ccaattccaa agaatcaaag
     4201 agactaacaa tacgcttccg attcaaaaaa gccgtcacat aggcttccgt atgaattgtc
     4261 gtaaaatttg gaaggtccgc atcgtatatt ttttccgctt caacagcaag atattttcta
     4321 ccttgtcgca tcacacgagc tgtttcctct ttaatcccgc ccacgggcgt caaacatccc
     4381 atccacagta cgcatcgaga attgatccgg gaaatcgttt ccgcatctgt ggacatcgta
     4441 tttttatcaa aagcataaaa gtttgccgag gaaaccgctg ggccatccaa gaccttcatc
     4501 agatgtttca cagatggagc atgcggcatc agtttttgtc gatactctcc aagggtaatg
     4561 acagataact caaaattaat tcttccaggg tattggtcaa ccaaccgatg aatcttggga
     4621 acatggacaa aaccaaccgt aaaaaattga atgtttttat cggtaaattt caaaaaatct
     4681 tcgatccact ccatggcctt tggatgaaga aacaaatcgg tccattccat gtattcatta
     4741 ccaccaaggc accagaactg aagaggatcc gttggcttgg agctaatata atttaaaatg
     4801 aactgccagt cctcttggga ggtttttggt gggtccagcg tttctcggta ggaatgatct
     4861 aattcataac aaaacgcaca tttgacagga cattcttttc ctaggctcaa tggaattttt
     4921 cctgcatcca tttcccgctt caacacgtcc cggtaatcgt gtccggcaaa tagctttgga
     4981 gcttctaata ttggaagttc acgcaatatc gtcaaccctt tctaaaaaaa gaccgactgt
     5041 gggtagcctg aaatccaatt gacagtacca ctgctccttt gaaacacttg agaatcttaa
     5101 tcggcctgct gagtattata agaccttctg ataaaaaatt cataccttct caagggattc
     5161 aaactttatg accctagaat acgcactgct tctcttcaat ctaagcgagc cgttttcgca
     5221 tgatgtctta cagcaacgct atgaagaagc caggcgaatc tggttcccat cacgttacgc
     5281 aggcctcact aataatccag cgaaatatat ggaaatgtac aaaaagggag agaccaaaac
     5341 taaagaaatt catgcagcct atcaaattct atccgatcat ctgcggaatt ccggttcctc
     5401 tccacaaaaa tatgatcacg gagcagaatg actgaccacc cgggacattc cgggctcacc
     5461 ttttgaattt ttctcggagg gcgcatgaga cacaggccaa gtgatgagac cttgaacccc
     5521 gtcagttcgt ggttcatcct gcccggcttt catcgcatag atctgaggta ggtaattcgt
     5581 accgaattta gaaatgtaga ggaatacatg ttcacgcgca ttgactcgaa cagcccatgg
     5641 ctcaaagtcc ttctgataaa actcctctgc taattgcatg gcctccaaac aagtgagccc
     5701 accaggttcc ttaagcgtgt aatagtaatc gagaggcata tcatactctt cttgcttatg
     5761 aatggtgata ccaaacttat cgggattccg aaccattggc gtatgccgat aagccacaaa
     5821 atagaataat tccacagagt gaatatatcg tttattatca agaagaaatt cccgtgtctc
     5881 ctcagcttca tctcgtgttt cacctggaaa tccataaaaa gccatgacat gattccagat
     5941 cccggctttg gcggcctcac gtagattatt ttcaatcaca gcctttttcg catgcttatc
     6001 catgagctcc aaaacacgct catttgctga ttccattcca taatacaagg tgcaacaacc
     6061 tgcttgaaca gcttgtttcc aaatttcagg atcctgcaat gattcctcaa accggattaa
     6121 agtcgtccat tttatcccta cctgctcctc aatcaacaat tgcgacactt ttttcaatag
     6181 cgcgggagga taggattcat ctgaaaataa aaagtgttct gcccgatact tctctttcaa
     6241 tgctttgatt tctcgtacta catcatgcgc ggattttcca cgatactggt cgaaatatcc
     6301 ctgtccatga tcacaaaagg tacatcgtcc ccagtagcac cctcgtgtgg ccaagtaagg
     6361 taaaattcga accgggacga aataggcatc caacggcaat ccatcaaagt ccggaagagg
     6421 caaggctgtt gtcttttcgg tatagatttc ctcattcaca cgaatcgccc ccccttgatg
     6481 ccacatcaag ttagggacct tttcccaatc tcgctccccc gccagagctt ccagcaacca
     6541 aaccaacgca tgctcgcctt catacaaaat ggccgagtca aagacttgcg taaaaaattg
     6601 cttccgttta ggaagatcat cttttaaccg ggtaatcacg ttgccaccca ccgtcacatg
     6661 aatgtcaggg aattcctctt taatcatttt acaaaaggtc atgcccgcca agagttgcat
     6721 ttgcgttcca acggaaactc ctatcacttc cggtttttct ttctgaatgg cagggagcac
     6781 taactgacgg cacacatccc gatacacgtt aacaagatcg tcatccaggc atgccaacac
     6841 ttcagtcgag actcccgagc gataccccag attactttcc atgggataga agacgaggga
     6901 agcaggaaaa taggctgcag aaataaattg catcacatct ctaaatgttt ttaaggccca
     6961 ttccaatttt tctgcatcat aaaatgcctc gcctctcacg atgactttgg cttcaatagc
     7021 tcgttcagcc aattccatca catctatagc atcttgcgat gccaaacagg ccagccggtc
     7081 tcgttcaaaa tcagtcaatt catttcgggc ttcccgttcg ttcaaacctc gtcgctgctg
     7141 atccattcga agcttgaccc agattaggaa ggtatcacta aaaaaccagt catacatttc
     7201 gatgttgaca tctttctgta tcacctcatg gccatatgag cgaagaacgg cagtcagact
     7261 gggcagggca agatatggag cggtgggaac ccattccgga ggaaacagta acatcgtttt
     7321 aaaaatccgc ctgggtttat cggtcgaacc accacgctcg atttcaatta aggattcagt
     7381 catatgatca acactcctga cattagacag aggctaattt cgatgaggaa tcatgtgagt
     7441 cgaggcgaaa ttgcagagaa ggtaatttat tggttccgaa gtgggtcacg tataaaaata
     7501 catattcccg aataaagatt ttcaaatccc accccgcata atgattttgt tcaaattctt
     7561 cgaatacgcg ttcggcatcc tcaacactca atccttcttt caccgtaaaa taataatcca
     7621 aggccaaatc ccattccgga tttttataat aggtaatgcc gaatttttca ggatttttcg
     7681 cgacgggagt atgcttgctc aaatcaaagg tcccaaatcc aattgaatgg acgtgttctc
     7741 gattatcttc taaaaattgg atagaaaatt tcgcatcttg gtatgtttcc cctgggaacc
     7801 caaaaaaccc catgacatga ttccaaaccc catgttggga ggacaactcc aagcttcgct
     7861 ggattacctc cgtcgtggtt gccttgtcca taagttgaag cacacgctca cttccggatt
     7921 catagcccat atggagaaat ttgcatccag aagtctgggc atccgcccag acttgatcct
     7981 caagaagact cttttcgaag cggatatgtg tggtccaggc aatatctaat ttcgactcaa
     8041 tcaatttctt cgtaagcttc ctaaataagg caggtggata agactcatcc gtgaaatgaa
     8101 aactccgggc atgatactta tttttcaaat aggtgagttc ttcaataatc tgccaatccc
     8161 ttttggtccg gtaccctgcc gtataaccct caccatggtc acagaactca caccgccccc
     8221 aataacatcc ccgagtggct aaatagggaa gaatcggctc aggtacaaag tatttctcta
     8281 atggcaatcc gtcgaaatc
//
LOCUS       DAORAQ010000118         8248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1364_length_8248_cov_64.016600, whole genome shotgun sequence.
ACCESSION   DAORAQ010000118 DAORAQ010000000
VERSION     DAORAQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_8248_cov_64.016600"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..992
                     /gene="hemB"
                     /locus_tag="PKM72_10220"
     CDS             9..992
                     /gene="hemB"
                     /locus_tag="PKM72_10220"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNP61207.1"
                     /translation="MGFPVHRLRRLRQHESLRRMVRETQLSPADFIYPLFVTFGENKQ
                     EPIVSMPGQWRWSVDRVIQEAKDAWELGIPAVILFGIPEHKDERGSSAYEKEGVVQRA
                     VRAIKDHVPDLMVITDVCIDEYTSHGHCGIVRDGRILNDETLECLQAMALSHAEAGVD
                     MVAPSDMMDGRVAAIRQALDQQGLSEMPIMAYSAKYASALYAPFRDAAFSSPSFGDRR
                     SYQMDAANAREALREVELDIQEGADIVMVKPALFYLDIIRRVRELVAAPVAAYQVSGE
                     YSMIKAGAQLGWIHEASVMMESLLAIKRAGADLLLTYFAKEAACLMNRDGL"
     gene            989..1960
                     /locus_tag="PKM72_10225"
     CDS             989..1960
                     /locus_tag="PKM72_10225"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNP61208.1"
                     /translation="MKITRGLPTVRPASCPVLTIGNFDGQHLGHRALVQAVVNCARQV
                     NGFPVVLSFAPHPVEVLRPGSVHKFLSEDYEKIAFFERLGVGELVILPFTMELASLGP
                     DEFVQQVLRDGLGIRKLFVGENFVFGRGRSGGVKDLIELGATAGFSVEPIAPLIVGQE
                     VVSSTRIRKCLTAGDVGQGAQCLGRPYMLEGIVIPGEKRGKQFGWPTANLRLPGHRVL
                     PSDGVYATLTVFKGECLDSIAYIGTRPTFSEEERLLEVHIFDKTLQLYGEDISVHFIE
                     SVRGDMVFANPQDLVSQMDHDGQRAREILRNYSGRPRIPAVVQGGSG"
     gene            1961..3388
                     /gene="tilS"
                     /locus_tag="PKM72_10230"
     CDS             1961..3388
                     /gene="tilS"
                     /locus_tag="PKM72_10230"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNP61209.1"
                     /translation="MNRLVPLLGMEHKIDQAIRAKGLLASGDRIVVAVSGGPDSVALF
                     RCLVELRTRWHWDICIGHVDHGFRGPESEGDAKFVEALGERFGVTVMVRRLYLNKYDA
                     KLKKKSLQEYARTARYQALEQMVLDRRATKLALGHTADDQAETVLMWMLRGSGTGGLG
                     GIPPKRGMHVVRPLLDIHRSEIVAYLEERQEDSRVDSSNIQPVYRRNHIRQHLIPQLK
                     QYSPGIVNVLTRQAHILRDEHAYLETLADEAFQRTCVSNRMGERHFDRMALLTVPLPL
                     RRRVVRQSLQILAGHHQGPRFDLVERVLDRIEHGQSGWTITCHGVRIAQEYDRLVMAS
                     CEEVNQPGTDYSSMMVTPLSIPGEVVWPLTGERFSISRISSPVIDGHANQSEVYLDPA
                     RFTPDLMLRRWAPGDTFCPKGLGGKQKKLQDFFSDLKVPRSKRTKVPLLVAPEGIVWI
                     GGLRADERFKVSSSTTSVIMAKMIM"
     gene            3402..3953
                     /gene="hpt"
                     /locus_tag="PKM72_10235"
     CDS             3402..3953
                     /gene="hpt"
                     /locus_tag="PKM72_10235"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNP61210.1"
                     /translation="MNQIFGKPLVTQEAMRARIKELGKQISLDYQGKDLLVVGVLKGA
                     YVFFADLVRVIRLPIQIDFLIAKSHKAIGSSAKKVKVWSELTEKINNRHVLLVEDIVD
                     SGVTAQFLVKNLMKKKPASVAVCALISKPANRKVEVDLRYVGFEVPPTYLVGYGLDFK
                     QKYRNLPYLAKLDPNLVQEDSQS"
     gene            4062..5879
                     /gene="ftsH"
                     /locus_tag="PKM72_10240"
     CDS             4062..5879
                     /gene="ftsH"
                     /locus_tag="PKM72_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNP61211.1"
                     /translation="MNSRVKNLLFWVFVCLFMILLFNLFTVPNQHPEDPVIFSDFMAH
                     LEKGDVERVIIKNNNISAILKDGTRIQTYSVEYPDLVKVLQEKSVQIEAKPPEDNPWY
                     ITFLLSWGPFVLFLGLWFFLMRQMQMGGNRALSFGKSRARLLTEEKKKITFADVAGVD
                     EAKEEVVEIIDFLKDPAKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILIAATNRPDVLDPALLRPGRFDRQVVVNRPDVRGRGEILK
                     VHTKKVPVSSDVDLEQIARGTPGFSGADLENLVNEAALWAARLDKKSVDQIDFENAKD
                     KVLMGVERKSMVLTEEEKRTTAFHEAGHALMAKLLPGTDPVHKVTIIPRGRALGMTMQ
                     LPIDDRHSYSKDFLYNTLSILFGGRVAEELIFKSVTTGAGNDIERATELARKMVCEWG
                     MSDKLGPLTFGKKDEEVFLGRDFGSRRDFSDQVALEIDKEVKRLVIESYERTTRMLTE
                     HIHTLRAIAEALLEKEVLDGIEIEKIVQQSSSLEQAAAV"
     gene            6319..7107
                     /gene="folP"
                     /locus_tag="PKM72_10245"
     CDS             6319..7107
                     /gene="folP"
                     /locus_tag="PKM72_10245"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNP61212.1"
                     /translation="MGILNVTPDSFSEDGRNLKIGAAIERAQQMIAEGVDLIDIGGES
                     TRPGASYVDEDVEIKRIFPILTVLRKLTNIPLSIDTRKAAVARKALDLGADIINDVSA
                     LQDDPRMAQLIQEYGAGVVLMHRQGHSVNMQHAPQYNDVVGEIKNFLSERVSMAQSMG
                     IPADHILVDPGIGFGKTLSHNLEILRNIGELLPLGQPILIGVSRKAFLGTLTGRPVGE
                     REFGNAVAVAAAVWQGAHMVRVHEVGAMHDAILVAQALQNSCDK"
     gene            7362..>8248
                     /gene="glmM"
                     /locus_tag="PKM72_10250"
     CDS             7362..>8248
                     /gene="glmM"
                     /locus_tag="PKM72_10250"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNP61213.1"
                     /translation="MGKLFGTDGVRGVANLDPMTSEMAMKLGRAAAHVFRKRDGRHKI
                     IIGKDTRVSGYMLESALTAGLCSMGVDVLLVGPLPTPAIAFMTRSLRADAGVMISASH
                     NPYQDNGIKFFSNDGMKLPDELEHRMEDLILSNEIEHIRPTAEAIGKAYRIDDAEGRY
                     IEFVKRSLPRAMDFQNMRIVLDCAHGAAYHVFPKVIRELGAKVWVMGNDPDGLNINDG
                     CGAVHPERLQQMVRDRKADLGIALDGDADRALFVCEQGQVVDGDHALAAFALDLKRLG
                     RLRHNTVVGTVMSNFGFEV"
BASE COUNT         2036 a         1652 c         2313 g         2247 t
ORIGIN      
        1 ggacgagtat gggatttccc gttcatcgtt tacgtcgttt gcggcaacat gaatctcttc
       61 gccgaatggt ccgtgagacc cagctgagtc ccgcagattt catctatcct ttgtttgtga
      121 cctttggcga gaataagcaa gaaccgatcg tctcgatgcc tggtcaatgg cggtggtctg
      181 tggatcgggt aattcaggaa gccaaggatg cctgggaatt agggattcct gccgttatcc
      241 tgtttggaat tcctgaacac aaagacgagc gtggatcttc cgcctatgag aaggaagggg
      301 ttgttcaacg tgcggtacgg gcgattaaag atcatgtccc cgatttaatg gtgatcaccg
      361 atgtgtgcat tgatgaatat acttcccatg gccattgcgg aattgttcga gacggccgga
      421 ttctgaatga tgaaacgcta gaatgtcttc aggcaatggc actgagtcat gcggaggcgg
      481 gagtggatat ggttgctcct tccgatatga tggatggccg ggtcgccgca attcgacagg
      541 cattggacca acagggactc tctgagatgc ccatcatggc ctattcggct aagtatgctt
      601 ccgcgttata tgccccgttt cgcgatgcgg ccttctctag tccatctttt ggggacagac
      661 ggtcctacca aatggatgca gccaatgcga gagaagcgtt gcgtgaggtg gaactggaca
      721 tccaggaggg tgctgatatc gtgatggtga agccagcctt attttatttg gatattattc
      781 gacgggttcg tgaacttgtg gccgctcccg tggcggcgta tcaagtaagt ggcgaataca
      841 gcatgattaa ggctggggca caactaggct ggattcatga agcttcggtg atgatggaaa
      901 gtttactcgc gattaaacgt gcaggggcgg atctgcttct tacatatttt gccaaagagg
      961 ctgcatgtct catgaaccgt gatggtttat gaagattact cgaggattac ctacggttcg
     1021 tcccgcctcc tgccctgttc tgaccattgg caattttgat gggcagcatt tgggacaccg
     1081 tgctcttgtc caggctgttg tgaattgcgc tcggcaggtc aacggatttc ctgtggtgtt
     1141 gtcctttgct ccccatcctg tcgaggtgtt gcgtccagga tccgtccata aatttttatc
     1201 tgaggattat gaaaaaatag cattctttga acgtctggga gtcggggaac tggtgattct
     1261 cccttttacg atggagttgg ccagtctggg gccggatgag tttgtccagc aggttcttcg
     1321 tgacgggctt gggatccgaa agctattcgt tggagaaaat tttgtattcg gtaggggacg
     1381 aagtggtgga gtgaaggatc tgattgaact gggggccaca gcaggttttt ctgttgagcc
     1441 gattgctccc ttgattgtgg ggcaggaggt ggtaagttcg acacgcattc gcaaatgcct
     1501 gacagctgga gatgtgggtc aaggtgccca atgtttgggg cgcccctata tgttagaggg
     1561 gattgtgatt cccggtgaga aacgtgggaa acagtttgga tggccgacgg ccaatcttcg
     1621 acttccaggg caccgagttc ttccttcgga tggtgtgtat gccacgctga cagtgtttaa
     1681 gggggaatgt ctggattcca ttgcctatat cggcacgcgg ccgacgtttt ctgaagagga
     1741 acgtttattg gaagtgcata tatttgataa gactcttcag ctatatggag aagatatttc
     1801 agtgcatttt attgaaagtg tgcgagggga tatggtgttt gccaatcccc aggacctcgt
     1861 gagtcaaatg gaccacgatg gtcagcgagc cagggagatt ttgcggaact attcagggcg
     1921 accccgaatc cctgcggttg ttcagggggg aagtggataa atgaatcgtt tagtccccct
     1981 tcttggaatg gaacacaaaa ttgaccaggc cattcgggca aagggcttat tggcgtcggg
     2041 agatcggatc gtggtagcgg tttctggcgg tccggattct gtggcccttt ttaggtgctt
     2101 agtggagctg cgaacccggt ggcattggga catctgtatc ggtcatgtgg accatggatt
     2161 tcggggacct gaaagtgagg gggatgcgaa gttcgtggag gctttaggcg agagatttgg
     2221 agtgaccgtt atggtgcgcc ggctttatct gaataaatac gatgcaaaat tgaagaagaa
     2281 atcacttcag gaatatgcca gaactgcgag atatcaggca ttagaacaaa tggttcttga
     2341 ccgaagggcg acaaagttgg cgctcgggca tacggcagat gatcaagcag agacggttct
     2401 gatgtggatg ctgcgcgggt ctggaaccgg tggattggga gggattcctc caaaacgagg
     2461 aatgcatgtc gttcgtccct tacttgatat tcatcggagt gaaattgtgg cgtatctgga
     2521 ggagaggcag gaggattcca gagtggattc ctctaatatt caacctgtgt accgccggaa
     2581 tcacattcgt cagcacctga ttcctcaatt gaaacagtat tcgccgggga ttgtgaatgt
     2641 gttgacccgg caggcacata tacttcgtga tgagcatgca tatctggaga cattagcgga
     2701 tgaagccttt cagcgcacat gtgtgtcaaa tcgcatggga gaacgtcact ttgaccgtat
     2761 ggcgctctta accgtacctt tacctcttcg tcgaagagtg gtccgccaga gccttcagat
     2821 ccttgcagga caccatcagg gtccgagatt cgatcttgta gaaagggtat tagaccgtat
     2881 agagcatggt cagtcaggtt ggacgattac atgtcatgga gtgcggattg ctcaggagta
     2941 tgaccgtttg gtgatggcct cctgtgagga agtgaatcaa cctggaacgg attattccag
     3001 catgatggtg acgcctttat ccatccctgg tgaggtggtt tggcctctca caggagagcg
     3061 tttttcgatt tcaagaatat cctctcccgt aatagatggc catgcgaacc aatcagaagt
     3121 ttatcttgac cctgcgagat ttactcctga tttgatgtta cgaaggtggg cgcctgggga
     3181 tactttttgt cccaaggggc ttggtgggaa acaaaaaaag ctgcaggatt ttttttcgga
     3241 tcttaaagta ccccgttcaa aacggaccaa ggtgccttta ttggttgcgc ctgagggaat
     3301 tgtctggatt ggtgggttac gagcagatga acggttcaag gtttcatcat ccacaacttc
     3361 ggtcattatg gccaagatga tcatgtaaga tggaagtctc tatgaatcag atatttggga
     3421 aacccctcgt gacgcaagaa gccatgagag ctcgcattaa ggaattggga aagcaaattt
     3481 ctctggatta tcaaggcaag gacctcctcg ttgttggggt cctcaaggga gcttatgtat
     3541 tttttgccga cctggttcga gttattcgat tgccgattca gattgatttt ctaattgcca
     3601 aaagtcacaa agcaataggg agttcggcaa aaaaggtgaa agtgtggtcc gagttgaccg
     3661 aaaagatcaa taaccgccat gtgttgctgg tagaagatat cgtggattct ggagttaccg
     3721 cacagttttt agtcaaaaat ttaatgaaga agaaaccagc ctccgtggct gtctgcgcgt
     3781 tgattagtaa gcctgccaat cgtaaggtgg aggttgattt gcggtatgtg gggtttgagg
     3841 tgcctccgac ctatcttgtg ggatatgggc ttgattttaa gcaaaaatat cgaaatcttc
     3901 cctacctggc taaactcgat ccgaatttgg ttcaagaaga ctcgcagtct tgatgcggtt
     3961 gtcagttata aaaagtgcgt gatagaatca attggttaag ctattgtctc gatagtgtgg
     4021 ctcattatga catttgaatt tctaaggggg ttaccctcgg tatgaattct cgggtgaaaa
     4081 acttactgtt ttgggtcttt gtgtgtttat tcatgatttt gttattcaac ctctttacgg
     4141 tgccaaatca gcacccggaa gatccggtga tttttagtga ttttatggct cacctggaaa
     4201 aaggggatgt tgaacgagtc atcatcaaga acaataatat cagcgcgatt ttgaaagatg
     4261 gtaccaggat ccaaacgtat tctgtcgagt atccagattt agttaaggtc ttacaggaaa
     4321 aatccgtaca aattgaagcc aaaccacctg aagataatcc ctggtatatc accttcctgc
     4381 tctcttgggg gccatttgtt ctatttttgg gcttgtggtt tttcctgatg cgtcaaatgc
     4441 agatgggcgg taatcgggct ctgtcctttg gaaaaagccg tgcgcgcctc ctgacagaag
     4501 agaagaaaaa aatcacattt gcagatgtgg cgggggtaga tgaagctaaa gaagaagtcg
     4561 tggaaatcat cgatttcctg aaggatcccg caaaattcca aaaattaggc gggcgtattc
     4621 ctaagggcgt tttgattgtg ggacctccag ggaccggaaa aaccttgttg gccaaagcga
     4681 ttgccggtga ggcaggggtg ccgtttttca gtatcagtgg atcggacttt gtagaaatgt
     4741 ttgtcggggt tggggcttcc cgggttcgag atctctttga acaggggaaa aaacatgcgc
     4801 cctgtatcat ctttattgat gaaattgacg cggtaggacg actacggggc gcaggtcttg
     4861 gcggaggtca tgatgaacgg gaacaaacac tgaatcaatt gttagtggaa atggatgggt
     4921 ttgacacgac ggaaggtgtg atcctgattg cggccaccaa cagaccggat gtgctcgatc
     4981 ctgccttgtt gcgaccaggc cggtttgatc ggcaagttgt ggtgaatcgt cctgatgttc
     5041 gtgggagagg ggaaattctc aaggttcata ctaaaaaagt tcctgtctcc tcagatgtgg
     5101 atctggaaca aattgccaga gggactccag ggttttcagg ggctgacttg gaaaatctgg
     5161 tcaacgaggc ggctctgtgg gcagctcgcc ttgataaaaa gtctgttgac cagattgatt
     5221 ttgaaaacgc caaagataag gtgttaatgg gtgtagaacg taagagcatg gttctgactg
     5281 aggaagaaaa gcgtacgaca gcgtttcatg aggctggcca cgcattgatg gcgaaactct
     5341 tgccaggaac ggatcctgtt cataaggtca ccattattcc tcgtggacgg gctcttggca
     5401 tgacgatgca attacccatc gatgatcgac atagttactc gaaggacttt ttgtacaata
     5461 cgctctccat tttatttggt gggcgcgttg cagaagaatt gatctttaaa agtgtgacga
     5521 ctggggcggg gaacgatatt gagcgggcca ctgaattggc caggaaaatg gtctgcgaat
     5581 ggggtatgag tgacaaatta ggtccattga catttggaaa aaaagacgaa gaagttttct
     5641 tgggtcgtga ttttggttca cgccgagatt ttagtgatca agttgcgtta gaaatcgaca
     5701 aagaagtcaa acgcctggtg attgaatctt atgagcgaac gaccaggatg ctgaccgagc
     5761 acattcatac attacgggcc attgcagaag cgttgttgga gaaagaggta ttagacggta
     5821 tagaaattga aaaaatcgtt caacagtcat cttcccttga gcaagctgcc gcagtataac
     5881 ggattttctg atgtgctttt cattcagaat agcgatgtaa ttctatttca aaactgagca
     5941 gatttgtttt tctgctcgtt cccctttttt atgaataata ggttgaaacg cctgcttcca
     6001 ttcagatcaa acttccctat ccctcgtgga tgaagagcat tttaacacgg gccccaccag
     6061 cattgtggtg gatttgccta gcgaacttaa ccgccaaact ttatccccca cgtatttttg
     6121 ttgaatttct cgatttccta gccattcaaa tcattgtgct ttataagatg gagagcctta
     6181 tatgattatt agagccaatc atggaaatga ttttgtcagc agccaagggc tttgaagccc
     6241 atgtctgttc caaagtccat atttcctgaa attcgctgtc gggagaaaag aatgcccatt
     6301 gggtcaaagg tgttaataat gggtatattg aacgtgaccc cagattcatt ttctgaggat
     6361 gggcggaacc tcaaaattgg ggcagccatt gagcgggcac aacagatgat tgccgagggg
     6421 gtggatttga ttgatatcgg tggggaatcg acgaggcctg gagcgtcata tgtggacgag
     6481 gatgtggaaa taaaaagaat ctttcccatt ctgacggttt tgaggaaact taccaatatc
     6541 ccactttcta ttgatacacg gaaagctgcc gtggctcgga aggcgttgga tctcggggcc
     6601 gatatcatca atgatgtgag cgctcttcag gatgatcctc ggatggctca attaattcaa
     6661 gaatacggag cgggggttgt cctcatgcat cggcagggcc atagcgtaaa tatgcagcat
     6721 gcaccacaat ataatgatgt cgtgggtgag ataaagaact ttttatctga acgggtctcc
     6781 atggcccaat cgatggggat tccggcagat catattttag ttgatccggg aatcgggttt
     6841 gggaaaaccc tcagtcataa tttagaaatt ctccgtaaca ttggggagtt gcttccattg
     6901 ggacaaccta ttttaattgg ggtgtcacgg aaggcattcc ttggcacgtt aactggaagg
     6961 ccggttggtg aacgagaatt cgggaatgct gttgcggtgg ccgctgctgt ttggcaggga
     7021 gcacatatgg tacgagtcca tgaggtggga gccatgcatg atgccatttt ggtggctcaa
     7081 gctttacaaa attcgtgcga caagtagtat aggagtatat tctccaagcg gcgtttccac
     7141 ccgtttgttc tgcttataac cgtcgtgatg agccatatgc ccaatattgg tcgccccctg
     7201 ggcgatgcct caaagaatga ctgtccttga ttaaagtgga aatttttgaa ttttctttcc
     7261 aatcccttga tttgctaatg ggtggagaga cagggaaaat actccactac tcaaattgtt
     7321 ctatggcctg agccaattac taagaaagaa aagggaacca catggggaaa ctattcggta
     7381 cggatggggt gcgtggagtc gcgaacctgg atccgatgac cagcgaaatg gcgatgaaat
     7441 taggacgggc ggcggcccat gttttccgaa aacgagatgg acgacataaa atcatcattg
     7501 gcaaagatac tcgtgtgtcc gggtatatgt tggagtcggc cttaactgcc ggcctatgct
     7561 ctatgggtgt ggatgtctta ttagtcggac ccttaccgac cccagcaatc gcgttcatga
     7621 cccgtagcct gagagctgat gccggggtga tgatttctgc ttctcacaat ccctatcagg
     7681 ataatggcat taaattcttt tcgaatgacg gaatgaagtt gccggatgaa ctggaacatc
     7741 gaatggaaga ccttattctt tcgaatgaaa ttgagcatat tcgaccaacg gctgaagcca
     7801 ttgggaaagc ctatcgcatt gatgatgccg aaggacgata tattgagttc gtcaagcgct
     7861 cgcttccgcg tgcaatggat ttccagaata tgcgaattgt cctggattgt gcccatggag
     7921 cggcttatca cgttttccca aaggtcattc gtgaactggg tgcgaaggtc tgggttatgg
     7981 gtaatgatcc tgatggtctt aatattaacg acgggtgcgg tgcagtgcac ccggaacgat
     8041 tacagcagat ggttcgagac aggaaggccg acctcggtat tgccctggac ggagatgcgg
     8101 accgggcgtt gtttgtctgt gagcaaggac aggtggtcga tggagatcat gctctcgcgg
     8161 cttttgctct tgacctcaaa agactaggcc gattacgaca caatacggtg gtgggaaccg
     8221 tgatgagtaa ttttgggttt gaggtctg
//
LOCUS       DAORAQ010000119         8207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1372_length_8207_cov_73.864696, whole genome shotgun sequence.
ACCESSION   DAORAQ010000119 DAORAQ010000000
VERSION     DAORAQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1372_length_8207_cov_73.864696"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..574)
                     /locus_tag="PKM72_10255"
     CDS             complement(407..574)
                     /locus_tag="PKM72_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61214.1"
                     /translation="MSEISALAAANFLNQAALPLIQAATIPQGNLSSSSSTQDTVSLS
                     SDAKTLHAHHR"
     gene            complement(702..2066)
                     /locus_tag="PKM72_10260"
     CDS             complement(702..2066)
                     /locus_tag="PKM72_10260"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNP61215.1"
                     /translation="MNISDRQSVHGQWSSRWTFILAATGAAVGLGNIWKFPYLTGQNG
                     GSAFVLVYILCVAALGIPLMMAEILLGRRGRSTPIRSMQILAKETNTTQWWQIVGWSG
                     TLAGMLILSYYSVIGGWTLAYIFKSAGGTFSGASGQFAADTFSSFVGSGTTLFIWHTI
                     FMVLTMGVVAGGVKGGLERAIQFLMPTLFILLLLMVAYSMTTGFFGKGLEFLFTPDFS
                     KLTGASMLTAMGQAFFSLSLGMGTIMIYGSYVPKDTSIASTSIIIGLADTFVALLAGM
                     AIFPIVFANNLEPGSGPGLIFQTLPVAFGNMPGGLAFGSLFFILLLVAAWTSSISLIE
                     PLVTWLIETFGLSRIKASLYSGFATWLIGLGTVWSFNWWAEIKLFGGTFFDLLDFVTS
                     NLMLPLGGILIALFAGWLMKAESTKSELNIHNPTLYLAWQALVRYIAPVAVFIVLLNA
                     IGIL"
     gene            2253..3743
                     /locus_tag="PKM72_10265"
     CDS             2253..3743
                     /locus_tag="PKM72_10265"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNP61216.1"
                     /translation="MSDEQRNRKVIALAPMPPEKSAYALARYSRSPDSIEESLRWVHS
                     HSSEKFWEQFYFAYGHGSIADLGHATVCFEQISELAAIRLEDEPLWDGQAKSSRYQNF
                     AAAGCYVPNEIANSEIEGEYRGILGSLYNIYRLLKDPIQTHLGKRYPRPETMKPADYA
                     RTIGARTYDVIRYLLPLAAMTNVGQVVSIRTLEKQITRLLSAQLPELQGIGEDLKEAC
                     AKSPSTVWAELHQEETKGLEPLAPTLARYAEANEYQGSVYRDVFRQVKGRLKTAGYDD
                     PKSWKGKDQPVDLLEPHAPLDELVTTLVYRVSHAPYRVLLEWVRQWSEKEKQEVVDAA
                     LRGRGPHDELIKEFRSGYAFVFDVLMDIGGWRDMHRHRRCQQIQQNFTTIHGYEVPSV
                     LAEAGVEREYRQAMDAVQSDIEQLRVSNQEAALYAIPFGFRVRCLFKMDFAEVEYIAK
                     LRSGVKGHWSYRTVAWLMKQKMLERHPYLGTLIEATSPDIEDSLTR"
     gene            3781..4800
                     /locus_tag="PKM72_10270"
     CDS             3781..4800
                     /locus_tag="PKM72_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61217.1"
                     /translation="MSVDYERVINDSICQGEWDVVYAEARRWSEQAECGPLPFFALNV
                     VCMLRGDFAEAWQVYPRAFGEETDVQILKEWIARLLSDRSDNPNFLLFEGVFHTQCGR
                     LEDAVVRYEQVGALAPHSPYPYFFLAQIYQVKDRTDQAVKAYREAIKRDPSYVAARLK
                     LGVAYQDQGQLEMAIPQYREVLKLRPDEPFGHTNLACALAEQGKLELAIEEYKKALKI
                     IPNDAEVHFALGGLYENRGRLDLAKRQYEEALRLEPNFAPAATAIGWFLYDKGQDNFA
                     MDYFSRALKANPNDAQATFGVGRIYDEKNKPELAVQHYQKALSLEKDPDRKMRILNFM
                     DKLLG"
     gene            complement(4857..5135)
                     /locus_tag="PKM72_10275"
     CDS             complement(4857..5135)
                     /locus_tag="PKM72_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61218.1"
                     /translation="MGTQTGLRLEQRGDGNYYRVILHIGSTLIPISDDIVEELRTHIA
                     LTPDQFFPIFVNKVGYSSYLQEQIRKEVQSTGDLIPQMSAIQQFFRQE"
     gene            5335..6417
                     /gene="serC"
                     /locus_tag="PKM72_10280"
     CDS             5335..6417
                     /gene="serC"
                     /locus_tag="PKM72_10280"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNP61219.1"
                     /translation="MSRLYNFSAGPAILPEEVLKQAQAELPDWHGSGASIMEMSHRGK
                     EFVSVAAEAEQDVRDLLGVPANYKVLFLQGGASTQFATIPMNILRGKSKADYILTGAW
                     GKKAVSDAKKYCAVNVAASSEGDNFTSIPPFERWALSQDAAYVHYTPNETIGGVEFHW
                     VPDTGAVPLVADFSSTILSRAIDVSRFGIIYAGAQKNIGPAGLTLVIVRDDLIGDVLP
                     ITPSVYDYAKQAEADSMLNTPPTYAVYIAGLVFKWLKKQGGLSAMGAINQRKAGKLYA
                     AIDGSKFYRNPVEKSSRSWMNVPFILANPDLDKAFITEAKEAGLTTLEGHRSVGGMRA
                     SIYNAMPEAGVDALIDFMADFEKRKA"
     gene            6622..8205
                     /gene="serA"
                     /locus_tag="PKM72_10285"
     CDS             6622..8205
                     /gene="serA"
                     /locus_tag="PKM72_10285"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNP61220.1"
                     /translation="MNILVSDSLSPKGVEVLERAGFSVDVKTKLTKEELLQEIPKYEG
                     LVVRSATKVTKDVIEAGTRLRIVGRAGTGLDNVDSEAATRRGIVVMNTPGGNTITTAE
                     HTMSMIASMSRKIPQATMSMKAGKWEKTRFMGAELYNKTLGLVGLGQIGSYVAKLAQG
                     WSMNVIGYDPYLAVDRAKQMGIEVVELNELFHRSDVISVHTPLTNETRGLINTETIGK
                     MKDGVMIVNCARGGIINEQDLCDALKSQKVAAAAFDVFETEPVDPKHPLMSLDNFTCT
                     PHIGASTEEAQENVAIGIAEQFVDYFKRGIARGAVNVPSVAPEVLPQLQPYLVLAERM
                     GRFQAQLLDGGIKSVTIEYFGEVAQLTVAPVTVAVLKGLLSPILEDVINYVNAPIVAK
                     ERGIEVKEVKSSDAGDFSSLIRIKVEAGPRTYSLGGTLFHRQEPRFVEINQFQVEVVS
                     EGQMILIDNVDRPGVIGMVGQILGQHDVNIARMQCARVERGASALLIIGLDAPLASAV
                     LDLLKKEKDILSVRMVDLS"
BASE COUNT         2077 a         1718 c         2302 g         2110 t
ORIGIN      
        1 gcagtgaggg tttatcataa attaaattta tgaaactcaa acggcttcca gcgaaatctt
       61 ctgaatgatc gcccggacac atagtgagta gcgtgccagg tcgattgggg tatgtgggtg
      121 gtctcaaggc ttttgggggc agggaatatt cacgtacaag gctgttggat taccgtaggt
      181 ataagctcgg catgtggagt gccgtggtgg tcgccggctg gaagagtggc tcgactcggt
      241 tccacttcaa gtagatggcc gtttttcctg aactgagttt ggtcttaggc ttgctggaat
      301 gaattcatcc gaataatcag tcaagcagta aggaggattc taaggaggga cctaaaaagt
      361 tcaatgtcgt cccgggattg aacttttcag gtgtcctaag tacgggctac ctgtggtgtg
      421 cgtgaagtgt tttggcatca gaggaaagcg ataccgtgtc ctgggttgaa gaagatgagg
      481 acaaattccc ctggggtatc gtggctgctt gaataagggg aagcgcagct tggttgagga
      541 agttggcggc agctaatgca gaaatttcac tcatgaggga gtctcctttc gctcaggagt
      601 cttgtcggat tgttctcagg aattcttgaa atatattctc ccaaaaatca ggatgtctgt
      661 ttgagactgc ggaaaattag gcaaacttgt cggtcttcag gctatagaat gccgatcgca
      721 ttgaggagga caataaacac cgcaaccggg gcgatgtaac gtacgagggc ctgccaggcc
      781 aggtagaggg taggattatg aatattaagt tcggatttgg tgctttcggc cttcatgagc
      841 cacccggcaa agagggcaat gagaattcca cccaggggaa gcatgagatt ggatgtcaca
      901 aaatctaaca ggtcaaagaa ggttcctccg aataatttga tttctgccca ccagttgaat
      961 gaccagactg ttccgaggcc aatcagccag gtggcaaacc ctgaatacag ggaagccttg
     1021 attcgtgata atccaaacgt ttcaatgagc caagtgacca gtggttcgat taaagaaatc
     1081 gacgacgtcc aggcggcaac aagcaggaga atgaaaaaga gcgaaccgaa ggcgaggccg
     1141 ccgggcatgt tgccaaacgc gacgggcagt gtttggaaaa tgagacctgg gccagagccg
     1201 ggttccaaat tgttcgcaaa cacgattgga aaaatggcca tgccggctaa taaggcgacg
     1261 aaggtgtcgg cgaggccaat gataatggag gtggaggcaa ttgaggtgtc cttggggaca
     1321 taggagccgt agatcatgat ggtgcccata ccgagactga gactaaagaa ggcttgcccc
     1381 atcgccgtca gcatgctagc gccagtcaat ttgctgaagt ccggggtaaa aagaaattcc
     1441 agacccttgc caaaaaaccc ggtggtcatg ctgtaggcga ccatgagtag caatagaatg
     1501 aaaagggttg gcatgagaaa ttgaatcgct cgttccagac cgcctttgac gccacccgcc
     1561 acgacgccca tggtcaaaac cataaaaatt gtgtgccaga tgaagagagt ggttccgctc
     1621 cccacgaaac tgctgaaggt gtccgcagca aattggccgc tggcgccact aaatgttcct
     1681 cctgcagatt tgaagatgta tgccagggtc cacccgccga tgacactgta ataggaaagg
     1741 atcagcatgc ctgccagagt cccggaccat ccaactatct gccaccattg agtggtgtta
     1801 gtttctttag ccaaaatctg catggaacga atgggtgtgg agcgtcctct acgccccaac
     1861 aaaatttcgg ccatcatcaa tggaattccc agggctgcca cgcagaggat ataaacgaga
     1921 acaaacgcac tccctccatt ttgtcctgtg aggtagggga atttccaaat attgcccaag
     1981 cctacggctg cacccgtagc cgcgaggata aaggtccatc gtgaggacca ttggccatgt
     2041 accgattgtc gatcggagat attcattgaa gatagaatta ttccctgaag attcagggga
     2101 gtgtagtgta ggtgccagac actttcaagg tttttaaagt atgcctggcg tagagataac
     2161 ctctgacttg agatgtcact cctgtctgac tatagtgtcc ccttcttctg tttcgagtgt
     2221 gtctttcgtg aggagttgga agggttgagt tgatgagtga cgaacaacga aatcgaaaag
     2281 tgattgcttt ggcgccgatg ccaccggaaa aatctgctta tgctttggct cgatatagcc
     2341 gttcgcccga ttctattgag gagagtctgc gatgggtcca ttctcattct tctgaaaagt
     2401 tttgggagca gttttatttt gcgtatgggc atggctcgat tgccgatttg ggtcacgcga
     2461 cggtgtgttt cgaacagatt tccgagctgg cggcgattcg ccttgaggat gaaccactgt
     2521 gggatggaca ggccaagtcc agtcgctatc aaaactttgc cgcagccggg tgttacgtgc
     2581 ccaacgagat tgccaatagc gaaatagagg gtgaatatcg cggcatcctt gggagtcttt
     2641 ataacattta tcggttgttg aaagatccga tccaaaccca tctgggtaag cggtatccca
     2701 ggccggagac gatgaagccg gcagactatg cccggaccat cggtgcccgg acttacgatg
     2761 tcattcgcta tctgcttcca ctggcggcca tgacgaatgt ggggcaggtg gtaagtattc
     2821 gtacgttgga aaagcaaatc acgcgtttgc tatctgctca attaccggaa ttgcagggaa
     2881 ttggggaaga tctgaaggag gcgtgtgcca aatcaccctc aacggtgtgg gccgagttgc
     2941 atcaggaaga gaccaaaggg ctagagccct tggcgccgac attagcccgg tatgcggagg
     3001 ccaacgagta tcaggggtcg gtttacaggg acgtgtttcg ccaggtgaaa ggacgattga
     3061 agacggcagg ctatgatgat ccgaaatcgt ggaaagggaa ggatcagccg gttgatttgc
     3121 tggagccgca tgccccgttg gatgaattag tgacgacgct ggtgtatcga gtgagtcatg
     3181 ccccttatcg ggtactcctt gaatgggtac ggcagtggtc agagaaagag aagcaggaag
     3241 tggtcgatgc ggcgcttcgt ggtcgtggtc ctcatgatga attaataaag gaatttcgat
     3301 ccgggtatgc atttgtcttt gatgttctga tggatatcgg ggggtggcgg gatatgcacc
     3361 ggcaccgacg ttgtcagcag attcagcaga attttacaac gattcatggg tatgaggtgc
     3421 cgtcggtttt ggcggaggct ggagtggaga gagagtatcg gcaggctatg gatgccgtgc
     3481 aatcggatat tgagcaattg cgcgtcagca atcaggaagc ggcactctat gccattccat
     3541 ttgggtttcg tgttcggtgt ttgtttaaga tggattttgc ggaagttgaa tatattgcca
     3601 aattacggtc cggggtaaag ggccattggt cgtatcgaac cgtggcttgg ctcatgaagc
     3661 aaaagatgct tgagcgacat ccgtatctgg ggactctcat tgaggctact tcccctgata
     3721 ttgaagattc gctgacgcga tagtatctcg gcagtattga actcgacaaa ggatgtgggc
     3781 atgagtgtgg attatgagcg tgtgatcaat gactccattt gccaaggtga atgggatgtc
     3841 gtctatgcag aggcacgacg gtggtcggag caagctgagt gcgggccctt gccgtttttt
     3901 gcgttgaatg tcgtctgcat gttgcgaggg gattttgccg aagcgtggca ggtctatcct
     3961 cgggcttttg gagaagagac cgatgtgcaa atacttaaag agtggattgc gcgcttactg
     4021 agtgatcgat ccgataatcc aaatttcttg ttatttgaag gcgtgtttca tacgcagtgc
     4081 ggccgactgg aagacgcggt ggtcaggtat gaacaagtcg gagctttggc tccacattct
     4141 ccctatccat actttttcct ggcgcagatt tatcaggtca aagaccgcac cgatcaagct
     4201 gtcaaagctt atcgcgaagc gataaagcga gacccttcct atgtggcagc tcgcctaaag
     4261 cttggagtgg cctatcagga ccaggggcag ttggaaatgg ctattcccca ataccgtgaa
     4321 gttttgaaac tccgtcctga tgaaccattt gggcatacca atcttgcgtg tgctctagcc
     4381 gaacaaggaa agttggagtt ggcaattgaa gaatataaaa aagcattgaa gattattccc
     4441 aatgatgctg aggttcattt cgccttggga ggtttgtatg aaaacagggg acgattggat
     4501 ttagccaaac ggcagtatga agaagctctt cggctggaac cgaattttgc tcccgctgcg
     4561 acggcgatcg gctggttttt atatgataag gggcaagaca attttgcgat ggattatttc
     4621 agccgagcgc tcaaagcgaa tcctaatgat gcgcaagcca cttttggcgt tggccggatc
     4681 tatgatgaaa aaaacaagcc ggaactggcg gtgcagcatt accaaaaggc actctcttta
     4741 gagaaggatc cagaccggaa aatgcgcatt ctcaatttta tggataaact gcttggatag
     4801 ggcagattgc cgcaatagct aatcggtaga ggacattctg gttggtaggg gagagtttac
     4861 tcttgcctaa agaactgttg aatggccgac atttgaggga tgagatctcc tgtgctctgt
     4921 acttcctttc gtatctgttc ttgtaagtag gaagagtacc ccaccttgtt gacgaaaatt
     4981 ggaaaaaatt ggtcaggcgt gagcgcgata tgcgttcgta attcctcaac aatgtcatca
     5041 ctgatgggga tcaaagtact gccgatgtgg agaataactc tatagtaatt cccatcccca
     5101 cgttgttcca atctcaaccc tgtttgtgtc cccaagttat tctcccacct tcttcatctc
     5161 tatgaaagcg ctctctattc gcgggtcatt gtagggtatc caaaattgga ataacaaggt
     5221 tccgtactct tcttgactgg tctgggaccc tgtgctagcg tacctccttt ttcacgctgg
     5281 tgaggccagt ggtttttcct ggggccagtt taagtatgga ggatgagtcc gataatgtcg
     5341 cggttatata attttagtgc ggggccggcg atcttgccgg aagaggtgct gaaacaggct
     5401 caggccgaat tgccggattg gcacggttcc ggtgcatcaa tcatggaaat gagccaccgt
     5461 gggaaagagt ttgtctcggt ggccgcagaa gccgagcaag atgtccgtga tttattagga
     5521 gtacccgcta attacaaagt gctctttttg caaggcgggg catcaactca atttgcaact
     5581 attcccatga atatcttacg tgggaaaagt aaggctgatt acattctgac tggtgcgtgg
     5641 ggcaaaaaag ccgtaagtga tgcaaaaaaa tattgtgcag taaatgttgc ggcatcttcc
     5701 gaaggcgata attttaccag tattccgccg tttgaacgtt gggcattaag tcaggatgct
     5761 gcctatgtgc attacacgcc aaatgaaacg atcggtgggg tggaattcca ctgggtgcca
     5821 gataccggcg cggtaccgtt agttgccgat ttttcatcaa ctattttatc gcgtgccatt
     5881 gatgtcagtc ggtttggaat catctatgct ggcgcacaaa aaaatatcgg ccccgctggt
     5941 ttaaccttag tgattgtgcg ggacgatctc attggagacg tgttgccgat tactccaagc
     6001 gtgtacgatt atgccaagca agccgaagcg gattctatgt tgaatacacc tccaacctat
     6061 gcggtgtata ttgccggttt ggtatttaag tggttaaaaa agcaaggtgg cctctcggcg
     6121 atgggtgcca tcaatcagcg gaaggccggt aaactctatg cggcaattga tgggtcaaag
     6181 ttttaccgca atccggtaga aaaatcctct cgttcctgga tgaacgtccc attcattttg
     6241 gccaatcctg atttagataa agcgtttatt acagaggcga aagaggcagg attaaccaca
     6301 ctcgaaggtc atcgctcggt tggtggaatg cgcgccagta tttataacgc gatgcctgaa
     6361 gccggagttg atgcgttaat agattttatg gccgattttg agaaacgcaa agcctaacgt
     6421 ctcctcggaa tcccggttac gaatgacttc ccagtatgat gccgatatgt aatgtgaata
     6481 cactatcagt gttcgtgggg ttgaatgaaa agtaggtgag tctctctggg tgtatgtgaa
     6541 tgagatgccc cattaacggg caagactcct cgatgttgct ttgtgcgaat ttaagtgggg
     6601 cctcccctat ttggagatac tatgaatatt ttagtgagtg acagcctttc ccctaaagga
     6661 gttgaagtct tagagcgggc cggattttct gtggatgtga aaaccaagct cacgaaagag
     6721 gaattacttc aagaaatacc aaaatatgaa ggtttagttg ttcggtcggc aaccaaagtc
     6781 actaaagacg tgatcgaagc aggaactcgt cttcgtattg tgggacgtgc cggaaccggt
     6841 ttggataatg ttgacagtga ggcggcaacc cggcgtggca ttgtggtcat gaataccccg
     6901 ggcggcaata caattaccac tgcggagcac acgatgtcga tgattgcttc gatgagtcga
     6961 aaaattccgc aggcgaccat gtccatgaag gcaggtaagt gggaaaaaac ccgctttatg
     7021 ggagcggaac tctataataa gaccttgggg ctggtaggtt taggccagat tgggtcgtac
     7081 gttgcaaaat tggctcaggg atggtctatg aatgtgattg gctatgatcc ctacttggcc
     7141 gttgatcgag ccaagcagat gggcattgaa gtggttgaat taaatgaatt gtttcatcgt
     7201 tctgatgtga tatcggttca tacgcctttg accaatgaaa cgagagggct catcaacacc
     7261 gagacaatag ggaagatgaa agatggcgtc atgatcgtga attgtgccag aggcggaatc
     7321 attaatgaac aggatttatg tgatgcgctg aaatctcaaa aagttgcagc ggcggctttt
     7381 gatgtgtttg aaactgagcc agtggatccc aagcatcccc ttatgtctct tgataacttt
     7441 acctgcaccc ctcatatcgg cgcttctact gaagaagctc aagagaatgt ggcgattggg
     7501 attgctgaac aattcgttga ttatttcaag cgaggcattg ccagaggggc tgtgaatgtt
     7561 ccttctgtgg caccagaagt ccttccccaa ctgcagcctt atttggtttt ggccgagcgt
     7621 atggggcgat ttcaggccca attattagat ggagggataa aatccgtcac cattgagtat
     7681 tttggtgaag tggctcaact taccgtggca cccgtgaccg tcgcggtgtt aaagggtctg
     7741 ctctctccta ttttggaaga tgtcatcaat tacgtgaatg ctcctatcgt ggcaaaagaa
     7801 cgtggtattg aagttaagga agtgaagagt agtgatgccg gagacttttc cagtctaatt
     7861 cggatcaagg tcgaagctgg tcctcgtacg tattcccttg gtggaacgct ctttcatcgt
     7921 caagagccac gttttgtcga aataaaccaa tttcaggtgg aagtagtctc ggaaggccaa
     7981 atgattttga tcgataacgt ggatcgcccc ggggtgattg gcatggttgg tcagatttta
     8041 gggcagcatg atgtcaatat tgccaggatg caatgcgcga gggttgagcg tggagcatcg
     8101 gcattgctaa ttattggtct ggatgcccca ctcgcttccg ccgtgttgga tcttctcaag
     8161 aaagagaaag atattctgtc cgttcgcatg gttgatctca gctagct
//
LOCUS       DAORAQ010000120         7903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1449_length_7903_cov_69.623981, whole genome shotgun sequence.
ACCESSION   DAORAQ010000120 DAORAQ010000000
VERSION     DAORAQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_7903_cov_69.623981"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="proB"
                     /locus_tag="PKM72_10290"
     CDS             complement(<1..955)
                     /gene="proB"
                     /locus_tag="PKM72_10290"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNP61221.1"
                     /translation="MREQVLAACKKIVVKVGSSLVASSQGGLRLDRINRLTQELGTLQ
                     TQNRQIVVVSSGAIVAGIAHLGISSYPTELPLQQAAAAVGQSRLMQAYEMAFEDTKNK
                     IAQVLLTHQDLSNRRRFLNARHTLNTLIQLGVIPIINENDTVAIDEIRFGDNDTLAGE
                     VAHLVDADLLVILSDVDGLYSQDPHLNPSAELIPLVANVTKDIEDLAGSSRTQASRGG
                     MVTKIKAAKQAGRFGVPTLLLNGETPNALGEALNGKPGGTFFASDQSPLTSRKQWIAY
                     TLRPKGELRLDEGAVAALTLRGKSLLPSGILDITGIFLTGDP"
     gene            complement(937..1956)
                     /gene="obgE"
                     /locus_tag="PKM72_10295"
     CDS             complement(937..1956)
                     /gene="obgE"
                     /locus_tag="PKM72_10295"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNP61222.1"
                     /translation="MFIDQARIFIKAGDGGTGHCSFRREKFAEFGGPNGGDGGDGGDV
                     VFVASNRVSTLLDLRYQKHYEATSGVRGLKANCHGANGSDILIALPVGTLVKTEDGDI
                     IADLIAEGQTAIVAKGGKGGKGNARFATSTNRAPRQFEPGTPGEERWVNLELKLLADV
                     GLVGFPNAGKSTLISAISSAHPEIASYPFTTLRPHLGVVEWMEHSSFVVADIPGLIEG
                     AHEGKGLGIKFLRHIQRTAFLLYLVDITEWISEDPIVIVETLQRELQAFDPELLERPF
                     AIVATKIDSQGNGQHLEALREYCATHHYPFFPISAVTREGLPSLVTYLGNAVLEAKTS
                     CASKC"
     gene            complement(2037..2291)
                     /gene="rpmA"
                     /locus_tag="PKM72_10300"
     CDS             complement(2037..2291)
                     /gene="rpmA"
                     /locus_tag="PKM72_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNP61223.1"
                     /translation="MAHKKGGGSSRNGRDSNPQYLGVKAYAGEKVTGGSIIVRQRGTK
                     FFPGTNVGLGSDYTLFAKVDGIVKFEGGIARRKVSVYPPV"
     gene            complement(2326..2643)
                     /gene="rplU"
                     /locus_tag="PKM72_10305"
     CDS             complement(2326..2643)
                     /gene="rplU"
                     /locus_tag="PKM72_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNP61224.1"
                     /translation="MYAIIETGGKQYRAEPNGILQIESLEGDVGSIIQFDSVRFVQTD
                     QAPIVGSPMISDAVVKGEIIRHARTRSITIFKKKRRKNYRRTKGHRQGFTQVRITEIV
                     ASA"
     gene            2808..3722
                     /locus_tag="PKM72_10310"
     CDS             2808..3722
                     /locus_tag="PKM72_10310"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNP61225.1"
                     /translation="MGTVSKKIWMDGALVDWDEASVHVLTHSLHYGLAAFEGIRCYEG
                     VTGSSIFRLEEHVDRLFESAHITMMPMPFTKKEVSDAIVETVRVNQLVSCYIRPIAYV
                     GYGAMGVYPGDNPIRLAIAAWPWGSYLGEDALAQGIRAKISSFTRHHVNVSMTRAKIS
                     GYYVNSILAKWEAKKSGYAECILLDPDGYVAEGTGENVFIVKKGVLKTTPLTSILDGI
                     TRNSILELARAQKIPVIEERFTRDAMYVADEIFLTGTAAEVTPVRELDDRKIGEGKPG
                     PLTKSLQDDFFRIVRGENAAYANWLTPI"
     gene            complement(3824..4195)
                     /gene="secG"
                     /locus_tag="PKM72_10315"
     CDS             complement(3824..4195)
                     /gene="secG"
                     /locus_tag="PKM72_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNP61226.1"
                     /translation="MYTLTVILHIIVCFLMIAAILLQAGKGAEIGASFGGSSQTVFGS
                     RGPGTFLSKITVGAAIVFMLTSLSLALLSKQANTSSTVIDLHPTSHHESSSPSTPAAT
                     PPTETDAVDPAAETTAPPTTP"
     gene            complement(4227..4985)
                     /gene="tpiA"
                     /locus_tag="PKM72_10320"
     CDS             complement(4227..4985)
                     /gene="tpiA"
                     /locus_tag="PKM72_10320"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNP61227.1"
                     /translation="MLRRFIVGNWKMHKTISQAESLVKDILRMYQPQASVELAIAPPF
                     ISLPAASRLLNSTPIKLAAQNTCANDEGAFTGEISPPMLRDVGCHYVIIGHSERRHIF
                     GETDQDINKKVHAVLHHALLPILCVGERLEERQSNKTQAVIQHQLQNGLKGLESNDLA
                     EITIAYEPVWAIGTGQAATVDQATEVHGEIRSFLATQWGIKPDQTRIIYGGSVTKENA
                     KSLFQSPQINGALVGKACLDSESFVKIAELATDN"
     gene            complement(5030..6229)
                     /locus_tag="PKM72_10325"
     CDS             complement(5030..6229)
                     /locus_tag="PKM72_10325"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNP61228.1"
                     /translation="MKHLKKQTIDQVDLRNKRVLIRVDFNVPLDEACQITDDSRIRAA
                     LPTINRAVDENAVVILCSHLGRPKGTIDQSLSLAPVAKRLQRLLNKPVEFVGDCIGPA
                     VEAVVNKAKPGDVVVLENLRFHPEEEKNDDQFSAQLASLADVYINDAFGTAHRSHAST
                     VGITKYVKVSAAGFLMKREVEALEGTVENPVRPFVAILGGAKVSGKIGVIENLGKVVD
                     KVIIGGGMAFTFIKAMGLEIGQSLVEKDMLDFAKRIHEQAMAQKIKFYLPVDCVVAAS
                     LDPEAETKIVPVQEIPAGWYGMDIGPASVRLFSEAVENAKTILWNGPMGVFERDAFSR
                     GTYAMAHSVANAYAKTIVGGGDTALAVYRAGESESMSFISTGGGAALQLLEGKHMPGL
                     AALPDRF"
     gene            complement(6305..7306)
                     /gene="gap"
                     /locus_tag="PKM72_10330"
     CDS             complement(6305..7306)
                     /gene="gap"
                     /locus_tag="PKM72_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNP61229.1"
                     /translation="MSIRVAINGFGRIGRNFFRTCFNDPAIEIVAINDLTDSKTLAHL
                     LTYDSIHGKFQADVQCGPDFLTVNGKQIQILAIKDPQALPWKEHQIDFVIESTGRFTD
                     RKGAGLHISAGAKRVIISAPAKDPDITIVLGVNDSNYNPQQHTIISNASCTTNCLAPV
                     SKVLLQEFGIRHGFMTTIHSYTNDQQLLDLPHKDLRRARAANLSMIPTSTGAAKALYL
                     VIPELKGKIDGVAIRVPTPNVSLIDLSVEVERDCDAETVNAAFQQAAQSSLKGYLQVS
                     EAPIVSSDLNGCSYSATVDTPLTSVIDKRLVKVFAWYDNEWGYSCRLHDLIKVLAQH"
     gene            complement(7512..>7903)
                     /locus_tag="PKM72_10335"
     CDS             complement(7512..>7903)
                     /locus_tag="PKM72_10335"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNP61230.1"
                     /translation="YGNTINLSDTEPVVRQKLKTMVTDPARVRRTDKGNPDVCPVFDF
                     HKIYSSADVIKQVDRDCRTAAIGCIDCKQQVADAMVERYGPMWDKRATILQHPEQTQE
                     MVEEGRKRASHVAHETMQEVKTAMKIL"
BASE COUNT         1891 a         1874 c         2041 g         2097 t
ORIGIN      
        1 tggggtctcc tgtcagaaag atgcctgtga tgtccagaat gcctgagggt aataaacttt
       61 ttcctcgaag ggtgagtgcg gccacggccc cttcgtccaa acggagttcc ccttttggtc
      121 gaagcgtata ggcaatccac tgctttcgac tggtcagggg tgattggtct gacgcaaaaa
      181 acgttcctcc aggctttccg ttcagggcct ctcctagggc attgggagtt tctccattta
      241 ataggagggt agggacccca aaccgtcctg cctgcttggc ggctttaatc ttggtgacca
      301 tgcctccacg gctggcttgt gttcgagaag atcccgcaag gtcttcaatg tccttggtga
      361 cgttggcaac cagagggatt aattccgccg aaggattgag gtgaggatcc tggctataga
      421 gcccatcaac atcggataga atcaccagga gatctgcgtc gactaggtgg gccacttctc
      481 cagcgagggt atcgttgtcc ccaaatctga tttcgtcgat cgccacagtg tcattttcat
      541 tgataatcgg aatgactcct agctgaataa gggtattcaa ggtatgacgc gcattcaaaa
      601 atcgtcgacg gttggataaa tcctggtgag ttaagagaac ctgggcaatc ttgtttttcg
      661 tgtcttcaaa cgccatctca taggcttgca tgaggcggct ttgtccgacg gcagcggcgg
      721 cctgctgaag gggtaattca gtgggataag aagaaatgcc gaggtgtgcg atgccggcaa
      781 cgatggcccc ggaggaaacc acgacaatct gtcgattctg agtctgcagg gtgcccaatt
      841 cctgggttaa acggttaata cggtcgagac gtaaaccgcc ctgcgaagaa gccaccaggc
      901 tgcttccaac tttgacgacg atcttcttac acgccgctag cacttgctcg cgcatgaagt
      961 ctttgcttcc agaacggcgt tccctaaata ggtcacaagt gatgggaggc cttccctggt
     1021 cacggccgaa atgggaaaga atggatagtg gtgtgtagca caatactccc gtagagcttc
     1081 caagtgctgc ccgtttccct gtgaatcaat ttttgtggcc acgatggcaa acggtcgttc
     1141 aaggagttcg gggtcaaagg cctggagttc tctttgtagc gtttccacga tcacaatggg
     1201 atcttctgaa atccattcgg tgatatcgac cagatataac aaaaaggcgg ttcgctggat
     1261 atgtcgcagg aatttaattc ctaacccttt cccctcatga gcgccttcga tcaatcctgg
     1321 aatatcggcg acgacaaatg acgaatgttc catccattcc accactccca aatgaggtcg
     1381 aagagtcgtg aacgggtaac tggcaatttc tggatgggca gaagagatcg ctgaaatcaa
     1441 agtggatttg ccggcgtttg gaaagcccac caaaccgaca tcggctaaca gttttaactc
     1501 cagattcacc catcgctctt cgcccggggt ccctggttcg aattgacgtg gcgctcgatt
     1561 tgttgacgtg gcaaaacgtg cattgccctt tccgcctttg cctcctttgg ccacaatggc
     1621 ggtttgtcct tctgcaataa gatcggcaat gatgtcgccg tcctcggttt tcacgagagt
     1681 tccgacagga agggcaatca gaatatctga gccattggct ccgtggcagt tcgccttgag
     1741 gcctctgacg ccggacgtgg cctcgtaatg tttttgatat cgcaagtcga gcaaggttga
     1801 gactctattg gaggccacaa acaccacgtc tcctccatca ccaccgtcgc caccattggg
     1861 accaccaaat tcagcaaatt tttctcgtcg aaagctgcag tgtccggtgc caccgtcacc
     1921 cgctttgatg aatatccgtg cttgatcaat aaacatactg atccttaaat cctagcggac
     1981 ttacaagaga caaatgaaga agatgaattg aaaaaagcag gaaacgggaa gggaagctag
     2041 acgggaggat acacgctcac cttgcggcgg gcgattcctc cttcaaactt aacgatcccg
     2101 tctactttgg caaagagtgt gtagtcgctt ccaagtccga catttgtccc aggaaagaat
     2161 ttcgttccgc gttgacgaac gataatactt ccgccagtca ccttttcacc ggcataggct
     2221 ttgacgccta aatattgtgg attgctatcg cgaccgttgc gggatgatcc gccgcctttt
     2281 ttatgtgcca tgagtcaaac ctccaaagaa tttcaaagac caatgttaag cagatgccac
     2341 aatttcagtg attcgaacct gcgtgaatcc ctgacgatgg cccttggtcc gtcgatagtt
     2401 ttttcgtcgt ttctttttga aaatggtaat ggaccgggtt cgcgcatgtc ggataatctc
     2461 ccctttgaca actgcatctg aaatcatggg agagccgaca attggtgcct ggtccgtttg
     2521 gacaaatcgc actgagtcaa attggatgat agatccaaca tccccttcaa gggactcgat
     2581 ttgtagtata ccgttcggtt cagcccggta ttgttttcca cctgtttcga taattgcata
     2641 cataagttta gctccttaac aaacacgcat tttgccagta tgtttacatt tatgtcaaga
     2701 ttttagtata tcatcacgcg ttcttggtgt ctggaaaaaa atccgtgatg cccttggcac
     2761 ccacgccttg tttttttaaa gcttatgtca atcggagaag tatttctatg gggactgtgt
     2821 caaaaaaaat ctggatggat ggagctcttg tcgactggga tgaggcctcg gtccatgttc
     2881 ttactcattc tctccattat ggtctcgcag catttgaggg gatccgttgt tatgaaggcg
     2941 tgaccggatc aagcatcttt cggttagagg agcatgtcga ccggctgttt gagtctgccc
     3001 atatcaccat gatgcccatg ccttttacca aaaaagaagt gtcggatgct attgttgaga
     3061 cggttcgagt taatcaatta gtttcgtgct atatccgccc catcgcgtat gtgggctatg
     3121 gcgcgatggg agtgtaccct ggtgacaacc cgattcgttt ggctattgcg gcttggccat
     3181 gggggtcgta tttaggggag gatgcgctgg cgcaaggcat cagggcaaaa atttcatcct
     3241 tcacccggca tcatgtgaat gtatccatga ctcgggcaaa aatatccgga tattacgtta
     3301 attcgatttt agccaaatgg gaagcaaaga agtctggata tgccgaatgt attctccttg
     3361 atcctgatgg ttatgtcgct gaggggacag gagaaaacgt gtttattgtg aaaaaggggg
     3421 tcttaaaaac gactcccttg acctctatcc ttgacgggat tacccggaat tcgattcttg
     3481 aattggcgag agcccaaaaa atcccggtga ttgaggaacg gtttaccagg gatgcgatgt
     3541 acgtagcaga cgaaatattt ctcaccggaa cagctgccga ggttacaccc gttcgcgaat
     3601 tggatgatcg aaaaatcggc gaaggtaagc cagggcctct cacaaaatcg ctgcaggatg
     3661 atttctttag aattgtgcgt ggcgaaaatg cggcttatgc caattggctg actcctattt
     3721 aggagtttcg cttctaacag gtggcctcat gcctcatgac catgggccat tccaccagtt
     3781 ttccagctgt taaagtagtt gccgactcac caaaggaaat agtttatggc gttgtcgggg
     3841 gggcagtcgt ctctgctgct ggatcaactg cgtcggtttc cgttggagga gttgccgcag
     3901 gagtcgacgg agaagacgat tcatgatggg aggttgggtg aagatctatc actgtggacg
     3961 aagtgttggc ctgtttagaa agtagagcca ggcttaggga tgtcagcata aacacaatag
     4021 ccgctcccac ggtgatttta ctcaaaaacg taccggggcc tcgacttcca aacaccgttt
     4081 gagaagatcc cccaaacgat gcgccgatct cagcgccttt tcctgcttgg agcaagatgg
     4141 cggcaatcat gagaaaacaa actatgatat gaagaatgac agtcaaagta tacataatgt
     4201 gtgcgcgttt attgccgtgt ttatgattaa ttgtcagtag ctaattcggc tatcttaaca
     4261 aaggattctg aatccaaaca agcttttccc acgagagccc catttatttg tggagattga
     4321 aagaggcttt tggcattttc cttggtcacg ctccccccgt aaatgattct ggtttgatcg
     4381 ggtttgatgc cccattgcgt ggcaagaaag gacctgattt ctccatggac ttcggtcgcc
     4441 tggtcgacag tagccgcctg gccggttcca atggcccaga ccggttcgta ggcgatggtg
     4501 atctctgcta gatcgtttga ttcgagaccc ttgaggccgt tttgtaattg atgttgaatg
     4561 actgcctgcg ttttattgga ttggcgttct tccagacgtt ccccgacaca taatattggt
     4621 aacagggcat ggtggaggac ggcgtggact tttttgttaa tatcctggtc ggtttctccg
     4681 aaaatatggc gtcgttcgga atgcccaata atgacatagt gacaaccgac gtcccgtaac
     4741 atcggtggcg aaatttctcc ggtaaaggcg ccttcgtcat ttgcacaggt attttgtgcg
     4801 gccaatttga ttggtgtgga attcagcagg cgtgaagctg ctggcaacga aatgaatggg
     4861 ggagcgattg ccaattctac cgaggcctgt ggttgataca tccggagaat atccttcacc
     4921 agcgactcgg cctgggaaat ggtcttgtgc attttccaat tgccgacaat gaatcgacgc
     4981 aacatgggga cctcttaatt tttggaagaa aaaaagcggg tcgctgagat caaaaacgat
     5041 cgggtaaggc tgcaagcccc ggcatatgct tgccttccag gagttggagt gcggcaccac
     5101 caccggtgga aatgaaggac atactttccg attctcctgc tcgatacacg gcgagagccg
     5161 tatcccctcc tcctactatc gtcttggcat aggcattggc cacagaatgg gccattgcat
     5221 aggtcccgcg ggaaaatgca tcccgctcaa agactcccat ggggccattc cacaaaatcg
     5281 ttttggcgtt ttcgaccgct tcgctgaaaa ggcgtactga ggccggcccg atgtccatgc
     5341 cataccagcc agcggggatt tcctgtaccg ggacaatttt tgtttccgct tcaggatcga
     5401 ggcttgcggc cacgacacaa tccacgggga gataaaattt aattttttga gccatcgcct
     5461 gttcgtgaat gcgtttggca aaatccagca tatctttttc gacgagagat tggccgatct
     5521 ccaatcccat ggccttgata aaggtaaagg ccatgccgcc accgatgatg actttatcaa
     5581 ccaccttgcc aagattttca atgaccccga ttttcccaga aacttttgcc cctcccaaaa
     5641 tggctacaaa cggacgaacg gggttctcca ccgtgccttc caaggcttcg acttcccgtt
     5701 tcatcagaaa tcctgctgcg gatactttga catatttggt gatgccaacg gttgaggcat
     5761 gagaccggtg ggcggttcca aatgcgtcat tgatgtacac atctgccaac gaggccaatt
     5821 gtgctgagaa ttggtcgtcg tttttttctt cttccggatg aaagcgcagg ttttctaata
     5881 cgaccacatc gccgggtttg gctttattga cgaccgcctc aactgccggc ccgatgcaat
     5941 cgccaacaaa ttcgactggt ttattcaaca gtcgctggag ccgcttggcg acgggagcca
     6001 ggctgagcga ctggtcgatg gttccctttg ggcgtcctaa atgagagcaa agaatgacga
     6061 cagcgttttc atccactgca cggttgatgg ttggaagggc cgcccgaatc ctggaatcgt
     6121 cagtgatttg gcaggcttca tcaagtggaa cattaaaatc cacgcgtatg agtacgcgtt
     6181 tgttccgaag gtcaacctga tcaatcgttt gttttttaag gtgcttcatg ccttcctttc
     6241 ccttccggtg ttgatggctg cttgtcgggg gaaagccgga tgttccttaa tgaatgcgaa
     6301 tgtatcaatg ttgagcaaga accttgatca ggtcgtgcaa gcggcatgaa tacccccatt
     6361 cattgtcata ccaggcaaac actttgacga gccgtttatc aatgactgaa gtgagaggtg
     6421 tgtccacggt ggctgagtat gaacacccat tgagatcact tgaaacaatg ggagcttcag
     6481 agacctgcaa gtaccctttt agcgaactct gagcagcttg ttgaaaggcc gcgttcaccg
     6541 tttccgcatc acaatctcgt tcgacttcaa cagaaagatc aatgagcgac acatttggag
     6601 ttggcactcg aatggctacg ccgtcaattt tcccttttaa ttccgggatg accaggtaca
     6661 aagctttggc cgctccggtg gatgtgggaa tcatggataa attggcggcc cgggcccgtc
     6721 gtaaatcctt atgaggcaaa tcaagcaatt gttgatcgtt ggtataagaa tgaatggtgg
     6781 tcatgaaccc atgccggatg ccgaattcct gtaaaagaac cttcgaaaca ggagcaagac
     6841 aattcgtcgt acaagaggcg ttggatatga tggtatgttg ctgagggttg tagttggagt
     6901 cattcactcc taatacaatt gtgatatccg ggtctttggc gggtgctgag ataatgaccc
     6961 gtttcgctcc ggcggatatg tgcagtcccg ctccctttcg gtcggtaaat cgaccggtgg
     7021 attcaatgac gaaatcaatc tggtgttctt tccatggcaa ggcctgaggg tctttgatgg
     7081 ccaggatctg aatctgtttc ccattgactg tcagaaagtc cggtccacat tggacatcgg
     7141 cttgaaattt tccatgaatc gagtcatagg tcaaaagatg ggccagggtt tttgaatcag
     7201 tcaggtcatt gatggccaca atctcgatgg caggatcatt gaaacaggtc cggaaaaaat
     7261 ttcttccgat gcgaccaaac ccattaatgg caacgcggat actcatagtt tgatacacct
     7321 aactaaatga gaataaggga atcttccata ggcataggat cgtaatttca caatagcatg
     7381 aagtcaagaa aatatcacaa ggctggctca agaaaattcc ccgttttcct tggttgtgag
     7441 ggccattatc ctttgtgtag tagaggaatt tttcttcgga aggtgctcgc ggattgggcg
     7501 atttgagatt gctagagaat tttcatggcg gttttcactt cctgcatcgt ctcgtgcgcc
     7561 acgtgagagg cgcgttttcg tccttcttcg accatttcct gcgtctgttc gggatgttga
     7621 aggatggtgg cccgtttgtc ccacataggt ccgtagcgtt ccaccatggc gtcggcaact
     7681 tgttgcttac aatcgatgca tccaatggcg gcggtgcggc aatcccggtc aacctgtttg
     7741 atgacgtcgg ctgatgaata aattttgtgg aagtcgaaaa ccggacagac atcggggttg
     7801 cccttgtcgg tccggcgaac ccgtgccgga tccgtcacca tggttttcag cttttgtcga
     7861 acgaccggct cggtatcgga aagattgatc gtattgccgt aac
//
LOCUS       DAORAQ010000121         7837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1459_length_7837_cov_68.307119, whole genome shotgun sequence.
ACCESSION   DAORAQ010000121 DAORAQ010000000
VERSION     DAORAQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1459_length_7837_cov_68.307119"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            807..1409
                     /locus_tag="PKM72_10340"
     CDS             807..1409
                     /locus_tag="PKM72_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNP61231.1"
                     /translation="MKSKFQARTSSQLPHRHLMLHKPYGVLSKFTDSEGRPTLADYVN
                     ELRVYPAGRLDMDSEGLLLLTSDGDLAHRLTSPFQKVSKTYLVQVERIPDESALVELR
                     EGVLVKGKRTRPAKVQLLHGEPDVYPRPVPIRFRKSVPTVWLRMEIQEGMNRQIRHMT
                     AQVGHPCLRIIRVAIGPIRLGSLSPGQWRELRTGELRGLS"
     gene            1897..4038
                     /locus_tag="PKM72_10345"
     CDS             1897..4038
                     /locus_tag="PKM72_10345"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HNP61232.1"
                     /translation="MSVTIILALSVFGLAIAYYYSSSVLKIPIDMGVEDPETRKRLAK
                     IHSAIATGAMAFLKQEYKIMAIFMVVFAAIIAVLIDDHHTDYVNEGLYTAIAFIFGAI
                     ISIASGFIGMKIATQGNVRTTVSANQSLSTAFNVALHSGAVMGFALVGLAALGLLIIY
                     LGLKSIMPDQLPTHILMEVIAGFGLGGSSIALFARVGGGIFTKAADVGADLVGKVEAG
                     IPEDDPRNPAVIADNVGDNVGDVAGMGADLFGSCAESTCAAMVISAVAFSGMVDALLY
                     PILISAIGIPVSLLTLLMVKVTSEDQVGPALKKMLIISSALMAVVMLFVTKAMLPDTF
                     EINGQAYTDLGVYFCFLTGLIAGLAVGLMTEYYTSHDYKPVREVAKSCETGAATNIIF
                     GLALGYQSAVGPYLAIGLAIYIPWMLAGMYGVAIASLGMLGTLVIALTIDAYGPVSDN
                     AGGIAEMAGMPEHVRKRTDVLDAAGNTTAAIGKGFAIGAAILTSLALFAAFLTRADLL
                     LKEQHGQDFDLLGSINLLDPLVFTGLFIGAVLPAYFSAMTMKSVGSAAYKMIEEVRRQ
                     FRTIPGLMEGKAEADYEQCVAISTQAALKEMIAPGILIMGTPLITGFLFGIQAVAGVL
                     AGSLVAGGVIAISSSNSGGAWDNAKKFIEAGNLGGKGTDTHKAAVVGDTVGDPLKDTS
                     GPSLNILIKLSAILSLVFVPFFVQYGGLLIK"
     gene            4574..5401
                     /locus_tag="PKM72_10350"
     CDS             4574..5401
                     /locus_tag="PKM72_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNP61233.1"
                     /translation="MESPNNLDDSFFQRKRRSEAIASAEVDLLLRVISQDLSAPMATI
                     QDTCRQLTMVGKRLNESFEEETSFGNARGPFDPLVTKEIPDAVQRIRGEVDHMRCLTS
                     GLLQFIRLGQIVLQWEGLDVNQLVMGIVTSMERKLKAKEVVVRIEDLPDCMGDEVLVN
                     QVFANLLDNAQKYLDPERPGEIIISGKAMHGWSVYSVGDNGIGLAEEHHARIFEAFSH
                     PVLEPEGRQGVGLTIVRRIIDRHQGNIEVESTPGQGSTFRVYFPRAIIAEEGGGKEE"
     gene            complement(6002..6469)
                     /locus_tag="PKM72_10355"
     CDS             complement(6002..6469)
                     /locus_tag="PKM72_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNP61234.1"
                     /translation="MRPKGSAEKLEARRRMAARLLAKGRGIREVARTIGAAPSSVKRW
                     KDALEQGGKNALNSKPHTGRPSRLSPKQRQNLLQILQRGPRAAKMRTDQWTCRLIGRM
                     IRRYFGVKYHPDHVSRILRGLGWKNKKNTSPVKKPSKTEQTRLRRLNQSRRHR"
     gene            complement(6660..7313)
                     /locus_tag="PKM72_10360"
     CDS             complement(6660..7313)
                     /locus_tag="PKM72_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNP61235.1"
                     /translation="MKLSKHVSPSDSSHIQTSTVPQPDQFSALLLVDLQNDFFPGGAL
                     AVPEADSLIPLINAYIKHFSRQGWPILATRDWHPANHSSFIEEQGPWPTHAVQGSRGA
                     QFHSDLVLPPGMMVISKGTDPKKDSKSGFDGTSLADRLEDLNIQTLYVLGLPTEHCVK
                     HTVLDGCQRGLRVVLLTDATKGGIPQQKDSLNPLQEMADAGACLANSSDLGISASSL"
     gene            complement(7319..>7837)
                     /gene="argB"
                     /locus_tag="PKM72_10365"
     CDS             complement(7319..>7837)
                     /gene="argB"
                     /locus_tag="PKM72_10365"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNP61236.1"
                     /translation="DGRLLTTKPFNPKSLIHTYSGSTDVLHPEDYGLVGQVSHVETAL
                     LHTLQEANFIPVIAPIGVDAKWKTQNINADLVAGSVAAAVQAEKLLVLSDVKGIRDAN
                     NRHVSTLSKKDMERMVKKQVISAGMLPKVHACLTALQGGVRKAHIIDGRVPHAVLLEI
                     FTDKGIGTEILA"
BASE COUNT         1778 a         1708 c         2181 g         2170 t
ORIGIN      
        1 agggggtctt gaccgcttcc tccccacgga tctttaggtt cccatgccat acgttagtcc
       61 tatgtttgag ttacaagcgg gatgtgcgag gtaattactg tagttacgtg aaccacggta
      121 acagaaattt ttgagtgggg caataaaaga tcaatgaatc ctgttccaca caggcaggga
      181 gtgccgtgtg gcaattccct ttctgcaatg gatttgctct atttgctatt ttagtaaatg
      241 gcctagtcag gtatttggcc ttggtccaag aaagaaaaat tcttattcct atggggagca
      301 aaaaattgga aaaatccttg gcggaatttt tcccatgaga tttgtggaga atggaggatg
      361 gattgaaggg taaaagactt ccatcacgta gtcgtcctga gggagttttc tacgggctca
      421 taaataggtg atgtcgattc aatcgccgta gagagcttgg tggtatggca acaaggaaat
      481 aagttgtatt ttttaggagg gatgtttttg acgacaggga gaagatctta gttgcctgtc
      541 gatgatttga gtgttttggg ggtaggccga gctttttcag ggcctgtcgt tcctacactg
      601 gagggcctgc cccctggtcc ttaccaaggg attgaaatgg acggagtaca gggaggaatg
      661 tttgtggtag acagggtgtt agctaaggga aagtcttgtg ggatctcccc agtctgatgt
      721 acttgctgga ataattgagt agggcagaga aaatgtccct tgggaagtgt ggagagacgt
      781 tttgtgattg accgacagcg ctgacaatga aatcaaaatt tcaagctagg acatcctcgc
      841 aattacctca tcggcatttg atgctgcata agccttacgg ggttctgtca aaatttaccg
      901 attccgaagg acgtccgacg ttagccgact atgtgaatga gttacgggtg tatccggccg
      961 ggcgattgga tatggatagt gagggcctgc ttctgctgac ttccgatgga gatctggctc
     1021 atcgtttgac ctcgccattt cagaaagttt ccaaaaccta tttggtgcag gtggaacgta
     1081 ttccagatga atcagccctc gtggagcttc gggaaggcgt tctggtcaaa ggtaagcgga
     1141 cccggccggc taaagttcag cttcttcacg gtgagccgga cgtctatcct cgccccgtcc
     1201 ccattcgttt tcgcaaatcc gttcccacgg tatggttgcg tatggaaatt caagaaggca
     1261 tgaacaggca gattcggcat atgactgcac aagtcggaca cccgtgtctc agaatcattc
     1321 gtgtcgccat cgggccgatt cggttgggct ctctttcgcc tggacaatgg agggagctgc
     1381 gaacaggaga attgcgcggg ttgtcctagg gctttctgaa tcgaatgtgt ggacaggaac
     1441 aagccattcg gatgaaataa ttagcaagag ccggaagcat ccctcatatt tgaccttgat
     1501 cagggtgttg gtgtcgataa aataggtatt agggttttta ccttgaacat tgttacgcag
     1561 acaggtaaaa ttcaccgctc gtcatcaatg aacatgcgtt gatggccgac ggaatagcgg
     1621 catgacacat ttttgaatca ggccgatgtc atgatcgtgt ctggtcggtc aaggacggtc
     1681 cccgcacgct gttttgtctc ccagctagaa cgacataagg aagttgattc gagaatggag
     1741 catttctctg tgaacaaatg aagggtcggt tcttagattg tgacgtatgg tcttagccca
     1801 tagccggaat tcaccggtcc cattctgtcg tttctgtcga gatgatgcta acaatttact
     1861 tctggtcact tacataagaa tggagatatt cccatcatgt cggtaactat tattctggct
     1921 ctgtccgttt ttggattagc tatcgcgtat tactattctt catcggtttt aaagatcccc
     1981 attgatatgg gtgtcgaaga tccggaaacc cggaagcgtc tggcgaaaat ccattccgct
     2041 attgccaccg gggccatggc attcctgaag caggaataca aaattatggc catttttatg
     2101 gtggtctttg cggcaatcat tgcggtgctc attgatgacc atcatacgga ctatgtgaat
     2161 gaagggctgt ataccgctat tgcctttatc tttggcgcca tcatttcgat tgcctccggt
     2221 tttatcggga tgaagattgc gacacagggc aatgtgcgga cgacggtttc tgccaatcaa
     2281 tctctctcca ccgcatttaa tgtcgcgctg cattccggag ctgtgatggg ttttgcgttg
     2341 gtcggcttgg cggctttagg actgttaatt atctatcttg gactcaaaag tatcatgccg
     2401 gatcaactcc cgacgcacat tctgatggag gtgattgccg gcttcggcct cggaggatcg
     2461 tccatcgcgt tatttgcgcg ggtgggtgga ggaatattca caaaagccgc tgatgtcggg
     2521 gccgatttgg tcggaaaagt tgaagcgggg attccggaag atgatccgcg taatcctgcg
     2581 gtgattgccg ataacgtagg cgataacgtg ggtgacgtgg ccggaatggg ggccgaccta
     2641 tttggttcct gcgcggaaag tacctgcgcc gccatggtga ttagtgcggt tgccttttcc
     2701 gggatggtgg atgcgttgtt gtatccaatc cttatttctg ccatcggcat accagtgagt
     2761 cttctcactc tgttgatggt caaggtcact tccgaggacc aggttggccc ggccctgaaa
     2821 aagatgctga tcatctccag tgcgctgatg gcggtggtga tgttgtttgt gaccaaggcc
     2881 atgctcccgg atacttttga aattaatggg caggcgtata cggatctagg ggtgtatttt
     2941 tgcttcctga caggtcttat tgccgggctt gctgtgggat tgatgactga gtactacact
     3001 tcccatgact ataagcctgt gcgggaagtg gcgaaatcct gtgaaacggg tgcagccacc
     3061 aatatcattt ttggactggc tctcggctat cagagtgccg tgggccctta tctcgccatt
     3121 ggtctcgcca tctatatccc gtggatgtta gccggcatgt atggggtggc cattgcctct
     3181 cttggtatgt tgggaactct ggtgattgca ctcacgatcg atgcctatgg accggtttct
     3241 gataatgctg gcggtatcgc tgaaatggcc ggtatgccgg aacatgtccg taaacggacg
     3301 gatgtgttgg atgctgccgg aaatacgact gccgccattg ggaagggctt tgccatcgga
     3361 gcggctattt tgacgtcatt ggccctgttc gccgcgttcc tcaccagggc ggacttgctg
     3421 ctcaaggaac aacatgggca ggattttgat ctgttaggca gtattaattt gctggatccc
     3481 ctggtgttta ccgggctgtt tatcggagcc gtgttgcccg cctatttttc cgccatgacg
     3541 atgaagtcgg tgggatcggc ggcttataag atgattgagg aagtccgacg tcagttccgc
     3601 accattcccg gcctcatgga agggaaagcc gaggcggatt atgaacaatg tgtcgctatt
     3661 tcaactcaag cggctttaaa agaaatgatt gcaccgggca ttctgattat gggaacaccc
     3721 ctgatcactg gattcctgtt tggcattcaa gcggtggctg gggtgcttgc cggttccctt
     3781 gtcgccgggg gcgtcatcgc catctcctca tcaaatagtg gtggtgcttg ggataatgcg
     3841 aagaaattta ttgaggcagg aaatcttggc gggaagggta ccgataccca taaggccgca
     3901 gttgttggtg ataccgttgg tgatcccctt aaagatacct cagggccgtc gttgaatatc
     3961 ctgatcaaac tttcagctat tttgtcactg gtgtttgtgc catttttcgt ccagtacggc
     4021 gggctattga taaagtaatt tctgcagact ataggcgccc aacaagtcct gagctttctt
     4081 ctgattggtt gggccgaatt ccgtgttcca tcggataatg ggtgctacag acggtgtttt
     4141 cattttaagt gatatagcga aactggttat agccttatat gtcgtattca tgcctatctt
     4201 gatgtggtat ttttcaatat tctattcggt gtggaatttt catctctctc tttatcctcc
     4261 ttttttcttc ctagctgtgg ttggccaagc tggtcatttc atcggtcatt cccctaattt
     4321 ttaccggtct tattacaaca aagattgatg ttctgaagtt ttcacgggcg ggatcgccag
     4381 tttggtgaaa taagaatagc catgaggcgc ttgggttttt ctcattcatg gttgttccgg
     4441 ttcgcatcga tttgagggaa tacgcattca gggggagggg gtgatctctc tccattggta
     4501 tgcgtcctgc ctaattctgg tagacaggtt tcctaatgaa tcgagatggc tgcttgaaaa
     4561 gggagtactt ggaatggaat cacccaacaa tctagatgat tctttctttc agagaaagag
     4621 aaggtctgag gccattgctt ccgcagaagt ggatttgttg ttgcgggtga tctcccaaga
     4681 tctttctgct ccaatggcaa ctattcagga tacctgtcgg caactcacca tggtgggcaa
     4741 acggctgaat gaatcatttg aggaggagac ctcattcggt aatgcacggg gtccattcga
     4801 tcctttggtc acaaaagaaa tccccgatgc cgtacagcgt atccgaggtg aggtggatca
     4861 tatgcgttgc ctcacgtcgg gacttctcca atttatccgg cttgggcaga tcgtccttca
     4921 atgggagggc ctggacgtga atcagcttgt aatgggaatc gtgacttcca tggagaggaa
     4981 actcaaagcc aaggaagtcg tggttcgtat tgaagatctt ccagattgta tgggtgatga
     5041 ggtattggtg aatcaagttt ttgcgaatct gcttgataat gcgcagaaat atctcgatcc
     5101 tgaacgtcct ggagaaatta ttatttccgg taaagcgatg catggatggt cggtctattc
     5161 tgttggggat aacgggatcg gccttgctga agaacatcat gcccgaattt ttgaggcatt
     5221 ctcgcatccg gtcctggaac cggaaggtcg tcaaggggtt ggattgacca ttgtgcgtcg
     5281 gatcattgat cggcatcaag gaaatattga agtcgaatcg actccggggc aaggctcgac
     5341 ctttcgggtg tattttcctc gagcgatcat tgccgaggag ggaggaggga aagaagaatg
     5401 aatcgaagga aataaaaacg tgtgattccc aatcgggcca attgaacttg accatcttca
     5461 cttggtcagc cacctccggc agaggccttt tcttgcttgg catggtttca tgaggaagtc
     5521 gtcagtcact gggtgcggct tagatcttct tttggtattc cgtgtattgg actgttgaat
     5581 attgtaaaat ttcgccgctt catcagctta ggagtcaacg aggctcatca gagaaaaata
     5641 gtggagtgtt caaaaaggcc cgtccagcaa ggccgcagcc gcttggacga gcggagcgta
     5701 cggaggagta cgtgagcacg gccaatgggc gagaacaccg ctggcggcat ttttcaacac
     5761 tccctgaatc gacgcgtttg ttgatgggac gtaggagcca ttcttcttgt gggccatgac
     5821 ctcttgcatg gcaaaaatgc tatccggcct gaagcttgtg tgtgaatgct gtagtaaggg
     5881 gcgccccttg cggcagtcgg gaaagtttgg agacaggcag caagaaagaa aaggtctttg
     5941 ctgggagtcg aaacccgtat tttgattgtt gaggaccgaa gtgaccctca tcgaaaattt
     6001 cttaccgatg gcgccgcgat tggttcaagc gccgaaggcg tgtttgttct gtcttgctgg
     6061 gttttttaac cgggctggtg ttctttttgt tcttccaacc taaccctcgc aaaatccgac
     6121 tcacatggtc tgggtgatat ttcactccga aatatcgccg aatcatccgg ccaataagac
     6181 gacaggtcca ttgatcagtc cgcatcttgg cagctcgtgg tcctcgttgg agaatctgga
     6241 ggagattttg gcgttgtttg ggcgaaaggc gcgatggacg accggtatga ggcttggagt
     6301 tcaaggcatt ttttccgcct tgttcgagtg catctttcca tcgcttaaca gaagatggcg
     6361 ctgccccgat tgtgcgagca acctcacgaa tacctcgccc tttggccaac agtctggcgg
     6421 ccattcggcg cctggcttct agtttttctg cactaccctt tggtctcata aatccacttc
     6481 tcggatttct caatgcgact cttgagggaa tatgtgcatc atcccgtaaa agaccaaagg
     6541 tgggatgcct ggaaaaaatt ccggaaggcg aggatgtgat gacagaaaaa attccccaat
     6601 tcaaaacgtg aggaaagtca accacatgcc taacacaaaa ggagtggagc agatactatt
     6661 cagagagaag aagcggatat tcccaaatcg ctgctgttcg cgaggcacgc gcctgcatcg
     6721 gccatttctt gtaggggatt cagggaatct ttttgttggg gaatgccccc cttcgtggca
     6781 tcagtgagca ggacgactcg aagcccgcgt tgacagccat cgagaaccgt gtgtttgacg
     6841 caatgttcgg taggtaagcc cagcacatac aatgtttgaa tgttgagatc ttccagtcgg
     6901 tcggccagac ttgtaccatc aaagccggat tttgaatctt tcttgggatc ggttccttta
     6961 gaaatgacca tcattccagg aggaaggact aagtctgaat gaaattgagc tccacgtgac
     7021 ccttgaactg catgagtcgg ccaaggtccc tgttcttcga tgaaggagct gtgattggcc
     7081 gggtgccaat ctctggtcgc cagtatgggc cagccttgac ggctgaagtg cttgatgtag
     7141 gcgttgatga gagggatgag gctatctgcc tcgggaacag ccagtgcgcc acccggaaag
     7201 aagtcatttt gtaggtcgac taaaaggagc gcggaaaatt gatcgggttg gggaacggtc
     7261 gaggtttgga tatgtgagga atctgatggg gagacatgct tggaaagttt catgttcgtt
     7321 atgccagaat ttctgtcccg atgcctttgt cggtgaaaat ttcgagtaac accgcatgag
     7381 gcacgcgtcc atcaataatg tgcgccttgc ggacgcctcc ctgaagcgcg gttaaacagg
     7441 catgaacttt ggggagcatg cctgcactga tgacctgttt tttgaccatt cgttccatgt
     7501 ctttttttga gagggtggag acgtgccgat tattggcatc acgaatgcct ttgacatcgc
     7561 ttaagaccag tagtttttcg gcttgaaccg ccgcagctac gctgcctgct accagatcgg
     7621 cattgatatt ttgggttttc catttggcgt ccactccaat tggcgcaatg accggaatga
     7681 aattggcctc ttgaagggta tggagcaatg cggtctcgac atgactgact tggccgacga
     7741 ggccgtagtc ttcaggatgc aacacgtcgg tcgatccgct gtaggtgtga ataagactct
     7801 tgggattaaa tggtttcgtg gtcaggaggc gaccatc
//
LOCUS       DAORAQ010000122         7696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1483_length_7696_cov_67.120010, whole genome shotgun sequence.
ACCESSION   DAORAQ010000122 DAORAQ010000000
VERSION     DAORAQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_7696_cov_67.120010"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1580)
                     /locus_tag="PKM72_10370"
     CDS             complement(72..1580)
                     /locus_tag="PKM72_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNP61237.1"
                     /translation="MPVLFHIAIFALSFGLMVGLGIAEEDVENTAEVTGEGAEEVDDT
                     RVAVAVKNRFSRVDPKSFSSIEVTVKHGVVTLTGTADSLWTQQRATELPQTIRGVRGV
                     IDRLSVGPVGRIDDSVLKKHLAYLFDENPVVERDNIHLDVQNGVLTLKGRVHSRQEKQ
                     TALNLAKMVKGIVELRDLLNVEGVRDRPDSVLKEEIIHRLAFDVWVVRPSLLKVQVKK
                     GHAILTGQVGSAYEKGRVAELAWVEGVHDVDVSGITVNWVSPDPMLRTQRPIFSDQEV
                     SNAIQGVLAYDRRVAPFHIHVSVKNGTVTLEGNVPFLSIRREAEQNANNTVGVQSVKN
                     LITVQSNATPKDADVQARLLEAFSRDPVLEQFTLGAVVKKGAVLLTGTVNSIYERNHA
                     ENVASRIQGVKSLTNQISFSTSEIKKSDWEIQLDFENQVWWSPFLSSQDIVATVEDGR
                     ATLSGSVQHIHQRLIAEQQAFEAGASTVINRLRVGNSPGSAESSSPRIPAGR"
     gene            complement(1709..5890)
                     /locus_tag="PKM72_10375"
     CDS             complement(1709..5890)
                     /locus_tag="PKM72_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61238.1"
                     /translation="MSSNQMAELIRNKDWTDTPLGDRANWPSQLKTSVQIILDSRFPM
                     FIWWGEHQINIYNDAYIPMLGNRHPEALGECASKIWADIWEVIDPQVQMVFQEGRATW
                     NEDLPLFMSRYGFLEETFFSFSYSPIKNDQGMVVGLFCAVTEETGRVLKGRRLDTLRQ
                     ISKVTERSDSVEEVCRLSADVLARSSLDIPFSLIYLWDAEERHVCLRAQSGLAPESSL
                     VPNRLEVDGDNFYPRPWWPVSFSPRWEEAAFSITVDLGASAGVWPEPCRQGMVLPLPS
                     WEREKKAGYLVVGISPRLAVNGRYFEFLNLLANGISGSINRAQALAEERKRNETLAEL
                     NRAKTEFFSNVSHEFRTPLTLILGSLDEAMQNTTFTTAPAVEVAHRNAMRMLKLVNTL
                     LDFSSLEAGRMKAVFEPVDVSGLTSDLASTFESAMKKAGLEYIVSCSTLSQPVYVDRS
                     SWEKIILNLLSNALKFTLQGRVYVEVIPHAEVVDVRIGDSGVGMNRETLSHVFERFFR
                     AKEALGRTFEGTGIGLALVKELVEMHGGKVHVESEPGEGSVFTVTVPFGFHHLPPEQV
                     SHTSTKPTDERLATSFILESEQWDGGIDHEPSGQRVLQDEGDSVQKTDRSRPIILLVE
                     DNGDMRDYMTRLLRADYEVVSACDGLEALEQVHMVHPHLILSDIMMPRLDGLELLKRI
                     RSNRDLQSTPFIFLSARAGQEAEVDGLLTGADDYLVKPFSAAELLARVKKTLALATMR
                     RAAIEYESRFQHLAHHPDVMTWITEADGNCIFLSQTWYDFTGQTEETGLGEGWMTAIH
                     PHDCPSIQQAFYEANRKKQSVRLEYRLRSARGEYHYVLDSASPRFDSDGGYLGYVGLV
                     LDLSYQKEMEEQQRESEERFRTLANTISQFAWMADSTGWIFWYNARWYEYTGTTLKDM
                     EGWGWKTVHHPDHVDRVVEKISRCFQTGEIWEDTFPLKSKDGEYRWFLSRAVPIRDQN
                     GKVVRWIGTNTDITELKTMQEQLQHLTAKLESRIARRTEELRHNQSRLQSLIGELILT
                     EQRERRRIAEELHDFLAQLLVACRLRVSSLEQMVGASGGLVSVLQEIDRILDQSLGYT
                     RSLVAQLVPSVLYQFGLLEALRWLAEDMKEYGLSVEVQMERDHLTLTENEAVLLFQSV
                     RELMLNVVKHAGVNRVCLAVTLSSPEEVCIEIADQGKGFDLHLIEQHSHSSNQFGLFS
                     IRERMSLLGGRMAIDSTSGSGTRIRLYASLPDQARPNAAFSSLAPAPMVATEISQESS
                     PERSSGFRVILVDDHAVVRQGLRSLLGAFSDIEVVGEAGDGIEAIRLTASLQPDVVVM
                     DINMPKMNGIEATRQIRHHHPGIHVIGLSVRQDKETEQAMRGAGAAAYLSKESAGQEL
                     YRLIVQLVRMHQKFS"
     gene            6255..7181
                     /locus_tag="PKM72_10380"
     CDS             6255..7181
                     /locus_tag="PKM72_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61239.1"
                     /translation="MKTLSPSMNGVINSPQEFNQFIPANFSYFLERSSSHIRRILQDM
                     GQWADNSAHEKLLLRLSFDLVERFIEYCPSQLPCRPSLLLDGFISDYFGRPQESKASS
                     TSNPILYRYLDGLLNRAVLSRDGLICLFYHLYGMKPVEVGFLLGLEEGQTQRIYKNFA
                     RWRQKGWFQATEEVGLTTQDVQSLVENQGANPELFNQQVRDNLQELLPFYRKSDPPYY
                     PCLEDSKWHEMLREGYGFDYRMWHLPLCLSCMNVITRIGDSFLLDPTITLNFHVSPHS
                     LKDEEISWRAHGSKPSSRNKTGHRYSRDLQMA"
     gene            complement(7294..>7696)
                     /locus_tag="PKM72_10385"
     CDS             complement(7294..>7696)
                     /locus_tag="PKM72_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61240.1"
                     /translation="TVMSKLLIIDPDSDSQKTLERLCDGYGVESVIAENLSDGICLYT
                     QESIDCIVIDLFLEQKSGFSFIAEITSQDSHPLIIATFSAARAPKFNIRRFAHLLGAS
                     YAFEKPLNPRLFLQAIGDLVSYIRIERDKGY"
BASE COUNT         1918 a         2126 c         1771 g         1881 t
ORIGIN      
        1 cattcgtcat tcatcactct caacgctgtg tggctgtgaa attacattgg tcctgatgac
       61 cagactcgca ttcaacgtcc ggcaggaatc cttggacttg atgattccgc ggaacctgga
      121 gagtttccaa cccgcaaccg attaatcacg gtcgacgccc cggcttcaaa agcctgctgt
      181 tcggcaatga gacgttggtg aatatgctgc acggaaccgg acaaggtcgc cctgccatct
      241 tccaccgtcg ccacgatatc ctggctcgat aagaatgggc tccaccacac ctggttttcg
      301 aaatccagtt gaatttccca atcagatttt ttgatttcag atgtggaaaa actgatttga
      361 ttggtcaggc tcttcacccc ttgaatccgg gaagccacgt tttcggcatg gtttcgttca
      421 taaatcgaat tgacggtccc cgtgagcagg acggctccct tcttcaccac ggccccaagc
      481 gtgaactgct ccaagacagg atctcgtgaa aacgcctcca ataaccgggc ctgaacatcg
      541 gcatccttag gagtcgcgtt agactggacc gtgatgaggt tcttcacgga ttggaccccg
      601 acggtgttat tcgcattttg ttcagcctca cgtctgatgg acagaaaagg cacattgccc
      661 tccagggtca cggtcccatt ttttaccgac acgtggatgt gaaagggagc cacgcggcgg
      721 tcataagcca gcaccccttg aatggcattg gaaacttcct gatccgaaaa gatgggacgc
      781 tgagtacgca gcatcggatc cggcgacacc caattgacgg tgataccgct cacatctacg
      841 tcatggacac cctcaaccca cgccaattcg gccacccggc ctttttcgta ggcgcttccc
      901 acttgtcctg tcagaatcgc atgccctttt ttcacctgaa cctttaataa ggatggacgt
      961 accacccaaa cgtcgaatgc cagacgatgg atgatttcct ccttcaggac cgaatccggc
     1021 ctgtctcgaa caccctccac attcagtagg tcccgcagct cgacgatgcc tttgaccatt
     1081 tttgctaaat tgagcgcagt ctgtttttct tgtctggagt gcacccgtcc cttcaaggtg
     1141 aggaccccat tttgaacatc aagatgaatg ttatcgcgct ccaccacagg attttcatcg
     1201 aacaggtatg ccaggtgctt tttgagaacc gaatcatcga tcctgccgac cggtcccact
     1261 gaaaggcggt cgatcactcc ccgaaccccc cgaatagtct gcggaagttc tgtggcacgc
     1321 tgttgcgtcc aaagactatc cgcagtcccg gtgagagtga cgaccccatg tttaaccgtg
     1381 acttcgattg aggaaaatga ctttggatcg actcgagaaa atcgattttt cacggccacg
     1441 gccacacggg tatcatcaac ctcttcagcc ccttctccag tcacctcggc ggtgttttcc
     1501 acatcctctt cggctattcc caagccgacc atcagaccaa aagacaaagc aaatatggca
     1561 atatggaaca gcacaggcat acccccattt ccatggaaca tacagaggaa agatgtggaa
     1621 tagaatgagg aaacgctcag aggatgtcag ttgacaaaat tggcgagcgt gaacaaaaaa
     1681 tgtcctggat tagaatctgc gttgaggctc aactgaattt ttggtgcatg cggactaatt
     1741 gaacaatcag gcgatagagt tcctggccgg cggactcttt ggataaatat gccgctgcac
     1801 cggctccacg catagcttgc tccgtttcct tatcctgacg aaccgataat ccgattacgt
     1861 gaatcccagg gtggtgatga cgaatctgcc tggtggcttc gataccattc attttgggca
     1921 tattgatatc catgaccacc acatcgggtt gcagagatgc tgtcagacgg atagcttcta
     1981 tcccatcccc ggcttccccc acgacctcaa tgtcggaaaa ggcgcccagc agacttcgca
     2041 aaccctgccg aaccacggca tgatcatcca ccagaatgac tcgaaatcct gacgaccttt
     2101 ccggacttga ttcctgactg atctccgtag ccaccatcgg ggccggagcc agagaagaaa
     2161 aggccgcatt cggtcgcgcc tgatcgggca gggatgcata caggcggatg cgtgtccctg
     2221 aaccggaagt cgagtctatg gccatccgcc cacctaataa agacatacgt tcccgaatac
     2281 tgaacagtcc gaattgattg gaggaatggc tgtgttgttc gatgaggtga agatcgaatc
     2341 ctttgccttg atccgcaatt tcgatgcaga cctcctccgg tgaggaaagg gtgacagcca
     2401 ggcacaccct gttcacacct gcatgtttca ccacgttcag catgagttca cgaacggatt
     2461 gaaacaggag caccgcctca ttctccgtga gggtcagatg gtcacgttcc atttgaactt
     2521 ccacagagag cccatattcc ttcatatcct cggccagcca ccgcaaggcc tctaacaacc
     2581 cgaattgata cagcaccgac gggaccagtt gggcgaccag agagcgcgta tagcccaacg
     2641 attgatcgag aatgcgatca atttcttgaa gaacagaaac cagacctccc gaagcaccca
     2701 ccatttgctc caggctactg acccgcaacc ggcaggccac aaggagctga gccaaaaaat
     2761 catgaagttc ctccgcaatc ctgcgtcgtt cccgctgttc cgtcagaatc agctccccga
     2821 tgagcgattg aaggcgagat tggttatggc gcagttcctc cgtacgtcgg gcaattcgac
     2881 tttccaattt ggctgtcaga tgttggagtt gctcctgcat cgtttttaat tcggtaatgt
     2941 ccgtattggt gccaatccat cgcaccacct tcccattctg atcccgaatc ggtacggccc
     3001 gggaaaggaa ccaccggtat tctccgtcct tgctcttgag aggaaaggtg tcttcccata
     3061 tttcacccgt ctggaaacac cggctgattt tctcaaccac ccggtcgaca tgatcaggat
     3121 gatggacagt cttccacccc cacccttcca tgtccttgag cgtggtaccg gtatactcgt
     3181 accaacgtgc gttgtaccaa aaaatccatc ccgtcgagtc ggccatccag gcaaattgag
     3241 aaatcgtatt ggcaagcgtg cgaaaccgct cttcactttc ccgctgttgt tcttccatct
     3301 ctttttgata ggaaaggtcc agcaccaaac cgacataccc cagatagccg ccgtccgaat
     3361 caaagcgggg agaggcggaa tccagaacgt agtgatactc cccccttgca ctccgaagac
     3421 gatattccag tcgaacggat tgtttttttc tgttcgcttc gtaaaacgcc tgttgaatgg
     3481 atggacaatc gtgaggatga atagccgtca tccaaccctc acccaggccg gtttcctccg
     3541 tctgaccggt aaagtcatac caagtttgac tgagaaagat acaattcccg tctgcttctg
     3601 taatccaggt catgacatcg gggtgatgag ccagatgctg gaagcgggat tcatattcga
     3661 tcgcagcacg acgcatcgtt gccaatgcca gtgttttttt cactctggcc agcagttctg
     3721 ccgccgaaaa cggtttgacc agatagtcat ccgcaccggt cagcagtcca tccacctcgg
     3781 cctcctgacc ggcccttgcc gacaggaaaa taaacggcgt cgattgcaag tcccgattgg
     3841 accgaatccg tttaagcaac tcgagcccat caaggcgagg catcatgata tcactcaaaa
     3901 tgagatgcgg atggaccatg tgaacctgct ccaaggcttc cagcccatcg catgcagaaa
     3961 ccacctcata gtccgcccgc aataatcgcg tcatatagtc ccgcatatcc ccattgtctt
     4021 ccaccaataa aatgatgggg cgtgagcgat ccgtcttctg aacggaatct ccttcatcct
     4081 ggagaactct ttgaccggac ggctcatgat ctatccctcc gtcccactgt tcggattcca
     4141 gaatgaaaga cgtcgctaaa cgctcgtcgg tgggtttcgt ggacgtatgg cttacctgct
     4201 ccggcgggag gtgatgaaag ccgaagggga ccgtcacggt aaacacactt ccctctccgg
     4261 gttcactctc aacatgaacc ttcccgccat gcatttccac caactctttc accaaggcca
     4321 gaccgattcc ggttccttca aatgttcgtc ccaaggcttc cttggccctg aaaaaacgct
     4381 caaacacatg agaaagcgtc tcccgattca taccgacccc ggaatcccca atccgaacat
     4441 ccacgacttc cgcatgggga atgacttcca cgtaaacccg tccttgcagc gtgaatttca
     4501 gagcattaga aagcaggttc agaatgattt tttcccagga acttcgatca acatagaccg
     4561 gttgagacag cgtggaacag gacacaatat attccaggcc ggcttttttc atggccgatt
     4621 caaatgtcga agccaggtca cttgtcaacc cgcttacatc caccggttca aacacggcct
     4681 tcatccgtcc cgcttccagg ctggaaaagt ccaatagcgt gttcaccagc ttcagcatcc
     4741 gcatagcatt ccggtgggcc acctccacag ccggagccgt agtgaaagtg gtgttctgca
     4801 ttgcctcatc caaggaaccg agaatcagtg tcagcggcgt tcgaaactcg tggctcacgt
     4861 tgctgaaaaa ttctgttttg gcacgattca gttccgccag tgtttcattc cgtttccgct
     4921 cttcggccag cgcctgcgcc cggttgatgg aaccggaaat cccgttggcg agcaaattca
     4981 gaaactcaaa atatctccca ttcaccgcta atctcggact gatccccaca accagatatc
     5041 cggctttttt ttctcgctcc cacgatggta aaggtaagac catgccctgg cggcagggct
     5101 ccggccagac tcctgcagaa gcacccaaat caaccgtaat ggaaaacgcg gcctcctccc
     5161 acctgggcga aaaggacacc ggccaccacg gccgtggata aaaattatct ccatccacct
     5221 ccaggcgatt cggaacaagg gaggattccg gcgccaatcc cgactgtgcc ctcaaacaca
     5281 catgacgctc ttcagcgtcc catagatata tcaaactgaa ggggatatcc agcgaactcc
     5341 gggcaagaac atccgctgac aaccggcaga cttcttcaac cgagtctgac cgttcggtga
     5401 ccttggaaat ttgccggagg gtatcgagtc ttcgcccttt taatacccga cctgtttcct
     5461 cggtcacggc acagaagaga cccacgacca ttccctgatc gttcttgatc ggactgtacg
     5521 aaaaagaaaa aaaggtttct tccaaaaacc cgtatctgct cataaaaagc ggaaggtctt
     5581 cattccaggt tgctcgacct tcctgaaaca ccatttgcac ttgcggatca atgacctccc
     5641 aaatatctgc ccaaattttc gaggcacatt cccccaaggc ctccggatgc cgatttccca
     5701 acatcgggat ataggcatcg ttataaatat tgatctgatg ctctccccac cagataaaca
     5761 taggaaatct cgaatccaga attatttgga cggaggtttt gagctgagag ggccagttgg
     5821 ctctatctcc caagggagta tccgtccaat ccttattccg gatgagttct gccatctggt
     5881 ttgatgacat tatggaaaaa gacctttcac ctgtatgagc ccctcaggtt aacggagaga
     5941 aaccagattt ttgactcatg ccccagattc gcaggctcaa ccctatcaca tctggcccat
     6001 tctttcagtt gacaaatcag ggtgtttcac ccgcttgagg gaaacaccct ctctgatttt
     6061 ctcacttttt ccctttcttg gaacctgatt gaggggacgt cctccctagg gtcgggatcc
     6121 ggagaatcaa gggattttaa cagtacggaa tgggggatca tttcgttaat ttgtacaaca
     6181 agacagatgg catgatttga agaaatgata tttatgtttc attcattctt tatcccccag
     6241 caaggaggcc gacgttgaaa actttatctc catcaatgaa tggtgtgatt aatagtcctc
     6301 aggaattcaa ccagttcatt ccggcaaact tttcctactt tctcgaacgt tcttcgagcc
     6361 atatccggcg tattttgcaa gatatggggc aatgggcgga caattcggca catgaaaaat
     6421 tattgttgcg tctttctttt gatctggttg aacggtttat cgaatactgt ccttcccaac
     6481 tcccctgccg gccttccctg cttttggatg gattcatatc cgattatttt ggaagacccc
     6541 aggaatccaa ggcgtcatca acatccaatc cgattttgta ccggtacttg gatggcctgc
     6601 ttaatcgagc cgtactgagt cgggacggtt tgatctgtct gttctaccac ctgtatggca
     6661 tgaaaccggt cgaggtcgga ttccttctcg gtttggaaga aggccagacc caacgcattt
     6721 ataaaaactt tgcgcgatgg cggcaaaagg ggtggtttca agcgacagaa gaggttggat
     6781 taaccacgca agacgtccaa tctttagtgg aaaaccaagg ggctaaccct gaattattca
     6841 atcaacaggt ccgggacaat ttacaagaat tgttgccttt ttatcgaaaa agcgaccccc
     6901 cgtactatcc ctgtctcgaa gattccaaat ggcacgagat gctcagagag ggatacgggt
     6961 ttgattaccg gatgtggcat ctacctctct gcctttcctg tatgaacgtc atcacccgga
     7021 ttggcgactc atttctcctc gaccccacta tcactttaaa ttttcatgtt tctcctcatt
     7081 cgttaaagga tgaagagatt tcatggcggg ctcatgggtc aaaaccttct tcgaggaata
     7141 aaacggggca ccgttattct cgggatctgc agatggccta acgtttctct aacaggtatc
     7201 tgaatatatt tggaagaatg ggaccacgat tacatacggt gaacagagcg tgaagagctg
     7261 tggtagacga atttttcctg accgtcttat gttttaatag cctttgtctc gctcaatgcg
     7321 aatgtatgac accagatcac caatggcctg aagaaataac ctggggttaa ggggtttttc
     7381 gaaggcgtag gacgccccca acaaatgggc aaatcttcta atattgaact ttggggcgcg
     7441 agcggccgag aatgttgcaa taatcagagg atgactgtct tgggaggtaa tttccgcaat
     7501 aaacgaaaac ccgcttttct gttcgagaaa caagtctatg acgatacaat cgatggattc
     7561 ttgggtatag agacaaatgc cgtctgaaag attttctgca atgacactct ctactccata
     7621 cccatcacag agacgttcca atgtcttttg tgaatcggaa tcggggtcga taataagtag
     7681 tttactcatc acagtc
//
LOCUS       DAORAQ010000123         7628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1498_length_7628_cov_63.724812, whole genome shotgun sequence.
ACCESSION   DAORAQ010000123 DAORAQ010000000
VERSION     DAORAQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1498_length_7628_cov_63.724812"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /locus_tag="PKM72_10390"
     CDS             complement(<1..2336)
                     /locus_tag="PKM72_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNP61241.1"
                     /translation="MITLFIPVQAQADPRSNAVNGKTPAQYVAETQGSAWAVIIGIDK
                     YLNLAGLAYAVDDAKSVARMLEGQGFKVTSLYNTQATRTAILRELRQNLLTRVQKNDR
                     VLIFFAGHIGETPGKDKQQPVGYMMPVDTEPGLLPDTAISVEILRELSEALPAKQVLF
                     LIDICYGGIAGRNQRSFPPMTGHYLEMITSEPARQLITAGGTGQQALAGPKWKHSVFT
                     YYLLEGIGKGRGDLNGDGIIPTSELFTFLDEHVQSAALTHKHAQRPEMWGLSPDKGEF
                     VFIPEKKSSGPQIANSPNRQGGSSNPVPGTIETIVGLLKSFGNPLDVFNNKVPSGKSE
                     QAQKQKSLAEEAATLEAEREKFELQALQTLKEQRQREKDLQAQLAEAQRLAAEEERRR
                     VAAEQARQEQAALLAQQQAELKAEQARVAEEEEQRRKKLAEQERTRLAQLAEAQRLAA
                     EEERRRVAAEQARQEKETLLAQQEAALEAQQARAAAEEEQRQLALAQLAEAKRVAAEE
                     ERRRVDAEKARHEQAILLAQRQAELEAQQAKLAAEEERRKQLLAQQERELQTQLAIAQ
                     RMVAEEERRRAEAEQARLAEEEERRKQALAQQELTRQAQLAEARRMAEEQARIEAEEQ
                     RRKEALAQRELTRQMQLAEARRMAEEQRRQEALAKQEQIRQAQLAEAQRMAAEEQERK
                     EALARQEQTRQAQLAEAQRMAAEEQERKVALARQEQTRQAQLAEAQRMAVEQARLKEE
                     AALKAEQARLAAEEQERRQAAEARRLAALEEERERVARL"
     gene            3061..4335
                     /locus_tag="PKM72_10395"
     CDS             3061..4335
                     /locus_tag="PKM72_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNP61242.1"
                     /translation="MTYWLMQHSLCRRIQPLACFFLLAMLVISPSVTHGKELKDVIPD
                     LYGGDGFQTDPNFAGNRSNDFGNSVFNSFNELTNGIGAQTGQFAVNSTVAAYRFDVER
                     GVPVEVTKSLGPLFAERADTLGKGRFDFQVVWSNANYKKFNGQKLSSLSEDVSPTAGP
                     GTPPNAPGLDELLQVNLDVKVITNFVGFFGTYGLTDNWDVNLLVPLINNQIKAKSKAT
                     MLDANGVPCTQGGNCSGIYVINGTAPGTGDPSKDSSSGNKTGIGDVLLRTKYNFLKNH
                     EILPDLGVRGGVSFPTGNEDNFMGVGEYKFEGLAVASKYYSSPIGMIGPHVNTGFVLV
                     NNKSELNLFTYVAGFDLAPIPVFAFSLDLLGRHELDDKDKSGKDIVDLAPGLKWAPYP
                     NTLLQAAVQLPLNKNEGLRPDAVWTLGLGGTF"
     gene            complement(4435..5484)
                     /locus_tag="PKM72_10400"
     CDS             complement(4435..5484)
                     /locus_tag="PKM72_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNP61243.1"
                     /translation="MNDENQSTLPAEKSSTPQADHTKPDRPGRQTYPVGTSSPKGRAP
                     SLNPHESSHAETVSMLKKQYQLAKRQKQWLKIAGFIGVFVMLLGGLGTWLAEENKDFK
                     MAILRVVSPVVSIHLDPEMVAIPAGTFQQGDSRGEDTLSEHPLRKVHIKKFALGRFEV
                     TFEEYDRFAMATDRAFPGDEGWGREKRPVINVSWEDARDYAKWLSEGTGLRYRLPTES
                     EWEYAAKNRAEDEIWAGTSEQEQLGTYAWFNTNSTGRTQPVGTRQANGLGLQDMSGNV
                     WEWVQDCWHDDYHRAPTNGLAWLEANDGRCGTRVRRGGGWTNDPISLRSSFRNGYNAD
                     SRSIQIGFRLAQDID"
     gene            complement(5983..6058)
                     /locus_tag="PKM72_10405"
     tRNA            complement(5983..6058)
                     /locus_tag="PKM72_10405"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6023..6025),aa:Thr,seq:tgt)
     gene            complement(6143..7096)
                     /locus_tag="PKM72_10410"
     CDS             complement(6143..7096)
                     /locus_tag="PKM72_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNP61244.1"
                     /translation="MRGLVFHQRLQFQPDLPIPPRPHGEARIRVIQAGICSTDLQLMK
                     GYMGFEGVLGHEFVGIVDEAPDTALIGKRVVGEINAACRACETCTNGHPTHCPHRTTL
                     GIQGRDGAFADFLTLPVENLFVVPDSISDDQAVFIEPLAAACEIPQLVSIKPTDRVVV
                     IGDGKLGILCAQVLVLSGCAVTLLGRHSDHDAWLTHMGIRITSHLTDISRGADLIVEA
                     TGSPEGLSTAMQLIRPRGTLVLKSTYHGNVSVNMADFVIQEISLIGSRCGPFPPAIRL
                     LETSHIRVDPLIHARYPLFDGVLAMEKADQSGIRKVMLQMT"
     gene            7343..>7628
                     /locus_tag="PKM72_10415"
     CDS             7343..>7628
                     /locus_tag="PKM72_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61245.1"
                     /translation="MMIAGMASALSGIQTGGRILSVGAHNTANAQTESFKRIRALPVE
                     SSAGNGVRVTWQTDERPGPQFLSSVDSISLREGSNVDLGKEIISNLQGVNL"
BASE COUNT         1702 a         1873 c         1933 g         2120 t
ORIGIN      
        1 agtcgagcga ctcgttcccg ctcttcttcc agagctgcca ggcgacgcgc ttctgcggct
       61 tgcctgcgtt cttgctcttc cgcggcgagt cgtgcttgtt cggctttcaa tgcggcctcc
      121 tccttcaggc gagcttgttc gaccgccatt cgttgggctt cggccagttg ggcctggcgg
      181 gtttgttcct gcctggccaa agccaccttg cgttcctgtt cttcggccgc cattcgttgg
      241 gcttcggcca gttgagcctg gcgggtttgt tcctgcctgg ccaaagcttc cttgcgttcc
      301 tgttcttcgg ccgccattcg ttgggcctcg gccagttgag cctggcgtat ttgttcctgt
      361 ttggccaagg cctcctggcg tcgctgttct tcggccattc gtcgggcttc agccagttgc
      421 atttgacgcg tgagttctcg ttgggccaac gcttctttgc gccgctgttc ctcggcctca
      481 attctggcct gttcctcggc cattcgtcgg gcttcagcca gttgagcttg acgtgtgagt
      541 tcttgttgcg ccaaggcttg ttttcgtctc tcttcctcct cggccagtct ggcctgttcg
      601 gcttccgctc gccgtcgttc ctcttctgct accattcgct gggctatggc cagttgagtt
      661 tgaagttctc gttcctgttg tgccaagagt tgtttgcgtc gttcctcctc agccgccagc
      721 ttggcttgtt gggcttctaa ttcggcctgt cgttgagcta agaggatggc ttgttcatga
      781 cgggcctttt ccgcgtccac ccgtcgccgt tcctcctcgg cagccactcg tttagcttcg
      841 gcaagttgcg ctaaggccag ttggcgttgt tcttcctccg cggccgccct ggcctgttgg
      901 gcttctaacg cggcttcttg ttgagcaagg agggtctcct tttcctggcg ggcctgttcg
      961 gcagccaccc tccgccgctc ttcctcggcc gccagtcgtt gggcttcggc cagttgggcc
     1021 agacgcgttc gttcctgttc cgccaatttt tttcttcttt gttcctcttc ttcggccacc
     1081 ctggcttgtt cggcctttaa ttcagcttgt tgttgagcca acagggccgc ttgttcctgg
     1141 cgggcctgtt cggcagccac cctccgccgc tcttcctcgg ccgccaggcg ctgggcttcg
     1201 gccagttgcg cttgaagatc tttctctcgc tgtcgctgtt ccttcaacgt ttggagagcc
     1261 tgtagttcaa atttttcccg ttctgcctct aacgtggccg cctcttctgc caaggatttt
     1321 tgtttttgag cttgctcact ctttccggat ggtaccttgt tattgaagac atccagggga
     1381 tttccaaaac tctttaagag tccgacaatt gtctcgatcg tccctggaac aggattgctt
     1441 gaacctcctt ggcgatttgg gctattcgca atttgaggcc cggaggattt tttttcggga
     1501 ataaacacaa attctccctt atccggactt aacccccaca tttccggtct ttgcgcatgt
     1561 ttatgggtga gagctgcgga ttgcacatgc tcatcgagaa aggtgaagag ttcggatgtg
     1621 ggaatgatgc catcgccgtt cagatcccca cggcctttcc cgatgccttc cagaagataa
     1681 taggtgaaca cactatgttt ccacttcggt ccggctaagg cttgttgccc ggttccgcct
     1741 gcggttatca attggcgggc aggttcactg gtaatcatct ctagataatg gccggtcatt
     1801 gggggaaacg aacgctgatt ccggccggca atgcctccat aacaaatatc aatgagaaat
     1861 aagacctgtt ttgccggaag ggcctcagac agttcacgaa gaatctccac gctgattgcc
     1921 gtgtctggaa gaaggccagg ttcggtatcg acgggcatca tgtagcccac tggttgttgc
     1981 ttgtccttgc ctggagtttc tccaatatga cccgcaaaaa agataaggac ccgatcattt
     2041 ttttgtaccc gggtaagaag gttctggcgc aactcgcgaa gaattgccgt tctggtggct
     2101 tgagtgttgt acagtgatgt gactttaaat ccctgcccct cgagcatacg ggccacggat
     2161 ttcgcatcat cgaccgcata ggccagaccg gcgagattca gatatttatc aattcctata
     2221 atgacagccc aggcgcttcc ctgagtctcg gctacatatt gggcaggagt ttttccgttt
     2281 accgcattgc ttcgtggatc tgcctgcgcc tgtaccggga tgaacaatgt gatcaaggca
     2341 aataccaaga aagagaggag aaagaagcgg ggatgggcaa ggaatcctct cctcggaatg
     2401 gaactatata aaacggaatc caacacgggg caccggctcc tgtgacgcat gtgtatttta
     2461 ttcaagaaaa atctcagaat gaaatagaca atggccaact ctctttttac ctcagatttt
     2521 ctcaagggct tccgatctcg ttgcgtcaga tcaccatagg gtcaagaatt gaaagaatgt
     2581 gtagcaggat taatagtggt cttgtggcgt tgtcagatct tgagaccagt ttactgggtc
     2641 ttgcgtcaga gatgcgagtg tggccttcat gtctggttca aaatttaagt gacgtttcgt
     2701 tccttcatgc agcctggcgg tctttttcct aaaaagaagc cactttgtct aatgggggaa
     2761 aggtagagcc ggtgcccctg ggaaatatgc attgacttca tgggaaagag tttatacgat
     2821 cgcctggttt tccctacttc ggcaacatcg cgttttttgg atatgaatcc atctgatagc
     2881 cacataagct gaatgaggga gtgaattaaa accctccttt tggacctgtg gtgttgagcg
     2941 taccatcccc caaacccata gttaaagtcg ataactgtgc tcgggcaatt gtgccaaatc
     3001 ctttgttcgg gatcgttcgg gaggcctctt tgggtttatt ctaatttatg aggagcaata
     3061 atgacctatt ggctcatgca gcattcttta tgtagacgaa ttcagccgtt ggcctgtttc
     3121 tttttgttgg caatgctagt catcagtcca tccgtcactc atggtaaaga attaaaggat
     3181 gtgattcctg atctttatgg gggggatgga tttcaaacgg atccaaattt tgcaggcaac
     3241 cggtccaatg atttcggtaa ttcggtgttt aattcattta atgaactgac caatggcatc
     3301 ggtgcacaaa cagggcaatt tgcggtgaac tcaacggtgg cggcgtatcg gtttgatgtt
     3361 gagcgtggag tgcctgtcga agtcaccaaa agccttggac cactttttgc ggaacgggct
     3421 gacacattgg gtaagggaag atttgatttc caggtcgttt ggtcaaacgc caattacaaa
     3481 aaatttaatg gacagaaatt gtcttctctc agtgaagatg tctctcctac cgccgggcct
     3541 ggaactccgc cgaatgcccc agggttggat gagctgttac aagttaacct ggacgtcaaa
     3601 gtgattacca attttgtggg attttttggc acatatgggc tgacggataa ttgggatgtc
     3661 aaccttttgg ttcctctcat caataaccag atcaaagcaa agtcgaaggc gactatgctt
     3721 gatgccaacg gcgttccctg tactcaaggt ggaaattgta gtggtattta tgtgatcaat
     3781 ggaacggcac cgggcacggg agacccttca aaagacagtt cttccgggaa taaaaccggg
     3841 atcggggacg tccttcttcg aaccaaatat aattttctga aaaatcatga aatcttaccc
     3901 gatttggggg tacgtggggg cgtttctttt ccaacgggaa atgaagataa cttcatggga
     3961 gttggagaat acaaatttga agggttggct gtggcttcta aatattattc atccccgatc
     4021 ggaatgattg ggcctcatgt gaacacgggt tttgtgctgg tcaacaacaa atccgaattg
     4081 aatcttttta cctatgtggc agggtttgat ctagctccca ttcccgtctt tgcgttttca
     4141 cttgatcttc tgggccgtca tgaattggat gataaggata aaagtggaaa ggacattgtg
     4201 gacttggctc ccgggttaaa atgggcccct tatcccaata cgttgcttca ggcagcagtc
     4261 cagcttcccc tcaataaaaa tgaaggcctc cggccggatg cggtctggac attgggactt
     4321 ggaggaacgt tctaaggtat tgaatcgtca cccctttggc tgctgccttt tataaggatg
     4381 ctttaggaca ggaagggatt tggaagaaaa taggaatatg ggagaatcga agtgctaatc
     4441 gatatcttgt gcaaggcgaa acccgatctg gatacttcgc gagtcggcgt tgtatccatt
     4501 tcgaaacgaa gaacgcaagg atatgggatc gttagtccag ccaccgcctc gcctcactcg
     4561 tgttccacat cgtccgtcat tcgcctctaa ccaggccaaa ccatttgttg gggcacggtg
     4621 atagtcgtca tgccagcagt cctggaccca ctcccataca tttccactca tgtcctgaag
     4681 tccgagacca ttggcttgtc gggttccaac cggctgcgtt cttccagtac tgtttgtgtt
     4741 gaaccaggca taagtaccaa gttgttcttg ttctgaggtt cccgcccaaa tttcgtcttc
     4801 tgctctattt ttggcagcgt attcccactc agattctgtt ggaagtcgat agcgaaggcc
     4861 ggttccctcc gataaccatt tggcataatc gcgagcatct tcccacgaga cattaatgac
     4921 cgggcgcttt tcacgtcccc acccctcatc tccaggaaac gcccgatccg tcgccatggc
     4981 gaaccggtca tattcttcga atgtgacctc gaatcgcccc aaagcaaatt ttttgatatg
     5041 gacctttcgt agcgggtgct cgcttaatgt atcctccccc cggctatcac cctgttgaaa
     5101 tgttccggca ggaattgcaa ccatttcagg gtcaagatgg atgctcacca ccggagagac
     5161 gactctcagt atggccattt taaaatcctt attctcctcc gctaaccatg tgcctaaccc
     5221 acccaatagc ataacgaaca cccctatgaa ccctgctatc ttcagccatt gtttctgacg
     5281 cttggcaagc tggtattgtt tcttcagcat ggacacggtt tctgcatgtg aggattcgtg
     5341 tgggttcagg cttggcgctc ggcccttagg cgaagatgtt ccgactggat acgtttgtcg
     5401 tccgggacgg tcaggctttg tgtgatcagc ctggggggtg gaagattttt ccgcaggtag
     5461 ggttgattgg ttttcgtcgt tcatggtgtg cgcaatctga gcatgcacat gcgaacagat
     5521 ttaagtcgct atagggacgg ttcttattct tctgcgactt ttcggtcaat gctgtcagga
     5581 aatgaccagt ttctccgtta aagcctgaag ccgtatttta taatttttta aaaagacgat
     5641 cctcatttca ccgtatttta acagatagtc ccctgaacga aagctttgtt tcttaatgta
     5701 aaaatttaat aagcattaca caagggcggt cccgcaaatt ttgataaaag gttaaattcc
     5761 tagattatta gaggaatgag gttgtttatc ctgactttcg aggatcagga ggtaagtgtc
     5821 agtttctgac acatcagaat actttgaacc cttgtgtcgg gcttagcgcg ttggaaaagg
     5881 gctgaaggga ctaatggagt ctttaccagg ataggagtgc gggtgcttct ttgataccgg
     5941 tacttttgca cctctgccga atcttgggaa acgtatgttg tgtggagctg gcgagaggaa
     6001 ttgaacctcc gacctgcggt ttacaaaacc gctgctctac cgattgagct acgccagctc
     6061 ggcgaatata aggaccatca taccaaagga tgaagcaatc aaccagcatg aatacataaa
     6121 aaattttcca accaaccctg cttcaggtca tttgcagcat gacttttcgt atgccactct
     6181 ggtccgcttt ttccatggcc agaacgccat caaaaagtgg ataacgggca tggatgagcg
     6241 ggtcgacccg gatgtgactt gtttccagta agcggatggc cggagggaaa ggaccgcaac
     6301 gagatccaat taaggatatt tcctgaataa caaaatccgc catattcaca gacacatttc
     6361 cgtgatacgt ggatttgagc acaagtgttc cccttggcct aatcaattgc atggctgtgg
     6421 aaagcccttc ggggcttcca gtggcctcca caatcaaatc tgcgcctcga gaaatatcag
     6481 tgaggtggga ggtgattctg attcccatgt gggtgagcca cgcgtcatgg tctgaatggc
     6541 gacccaacag ggtgactgcg catcctgata acacaagaac ttgtgcgcac aggatcccta
     6601 acttcccatc tccgatgaca accacccggt ctgttggttt aattgagact aattggggaa
     6661 tttcacaggc tgcggccagg ggttcaataa agacggcctg gtcatcggaa atagaatcag
     6721 gcacaacaaa aaggttttca acaggcaggg tcaggaaatc ggcaaaggcg ccatctcgac
     6781 cctggattcc taaggtggtg cgatgaggac aatgagtggg atgtccattg gtgcaggttt
     6841 cacaagcgcg acaagcggca ttgatctctc ccacaactcg tttcccaatc aacgcggtat
     6901 ccggagcctc atcgactatc ccgacaaatt catgccccaa gacaccttca aaacccatat
     6961 accctttcat cagctgtaga tcagtggagc aaattccggc ttggatgacc ctgattctgg
     7021 cttccccatg tggtcgcggg ggaatgggaa ggtctggttg aaattgaagc ctctgatgaa
     7081 aaaccagtcc ccgcatacgg tacctccatg aaattgccta gatcatggac acaataggaa
     7141 catagcaaaa aatgcaggca ctcttcagca atttctatga atatttcagt aagacgaatt
     7201 tccccggtat ggatagctat ttctccttca gtttgatgaa acatcttccg tcctgtcagg
     7261 aaaggtttct ggatttcggt cgataatgtg aaggtgtcgg gaggaaggac tgtgggatgc
     7321 ctgtcgaaac agaaggagaa caatgatgat tgccggtatg gcctctgcac tatctggaat
     7381 tcaaactgga ggaagaatac tcagcgttgg cgcccataat actgccaatg cacaaacaga
     7441 aagttttaaa cgaatccgtg ctcttcctgt agaatcgtca gccggtaatg gggtgagggt
     7501 tacttggcaa acagatgaac gccctggccc acaatttctc tcaagtgtag attcgatcag
     7561 tcttcgggaa ggatctaatg tcgatctagg gaaagaaatt atttccaatc tgcaaggggt
     7621 gaatctca
//
LOCUS       DAORAQ010000124         7616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1500_length_7616_cov_70.107525, whole genome shotgun sequence.
ACCESSION   DAORAQ010000124 DAORAQ010000000
VERSION     DAORAQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1500_length_7616_cov_70.107525"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKM72_10420"
     CDS             <1..476
                     /locus_tag="PKM72_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit beta"
                     /protein_id="HNP61246.1"
                     /translation="CVGKIRLQGLVKVGDDGLWAEDRWNPLYYAIRVEQVALPLYPQW
                     GTEPNGFYIPPRQAPRGYIRQMFGPGVDNAIEKYLVPSRELLAVLQLWRASQQIIFRY
                     DVIPGPKVFETQIHGRKFEMYNDTVLGFNKSGKEAVRQQVEEPIYIRPAERVNWL"
     gene            1150..2376
                     /locus_tag="PKM72_10425"
     CDS             1150..2376
                     /locus_tag="PKM72_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNP61247.1"
                     /translation="MMQLAKLKSELDDARLCTDRLFSLVSDEAMYERPIPERHRLIFY
                     LGHLEAFDWNQIGRWTLGMPSFHESFDQLFEAGIDPSLGNLPADRPSDWPTIHEVHRY
                     NARAREEVDRLLPTVPECIIHMAVEHRLMHAETNAYLLHHLDSKYKHVPANSSGGLSS
                     SFSEDIPKNEMMEVPEGMATLGREPEEGFGWDNEFLQHVVPVPAFLISRHKVTNQQYL
                     RFVQEGGEPSAFWVKRGDAWYVRTMFQEVPLPPFWPVYVTHRQAEAYANWAGMTLPTE
                     PQFHRAAFGTPSGIEQSFPWGNAASMFQHGNVDFHAWDLAPVNAHPLGDSAFGLAQMV
                     GNGWEWTSTVFHPFHGFSPFPTYPGYSARFFDQDHYVVKGAGPFTSSRLLRRSFRNWF
                     RQSYPYAHIGFRCVQS"
     gene            2432..3988
                     /locus_tag="PKM72_10430"
     CDS             2432..3988
                     /locus_tag="PKM72_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNP61248.1"
                     /translation="MRLILKRRGHWGILLFVLILWGNPAFATDRLVILHSSEHHGVAL
                     PLDQAGETRVGGLARRATIAEEVRREGVPVLMVDSGDILIGTAMSSWFRGEPDIRAMN
                     LIRYDAMVAGNHDFDYGIDHLRTLVELADFPILCTNLQSKRSVLPCRRSVVTRLGNVS
                     VGIVGIVGNSNFPDTFNRDVANTLSLMDPIASLQTEALRLRTEAHIDLVIALTHQDSN
                     EDLKILETVEGVDVLIGGHTEGFDGLYAPGLMEPVESVTRPRSVYVKTHRQGRTVGRL
                     DLTIEEGSVIQARSHNIPVTVEVPVEPNVQQLLSEYRQRFARQATQILGEASVRLQGD
                     RPVVRTQETNLGNLLADRMRRKFETDIALINSGQIRRSLEPGPVTLGDMLSVLPFDSA
                     LVTVHVTGAMLRQVLEHSVSQWPNHSGRFLQVSGLQVTYQGKAPVGSRVKSIMVGGMP
                     LDMSKTYTVATDAFVAEGGDGYDMLTHLSDRRDHQIPLRDLLLKALADGPLYAEADDR
                     IVFVNSFPSH"
     gene            4003..6414
                     /locus_tag="PKM72_10435"
     CDS             4003..6414
                     /locus_tag="PKM72_10435"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNP61249.1"
                     /translation="MSLPQAARDSRRWWADLVAVWRDTRQIVLTAQIAAIYAAILIPF
                     KAGIPIVPGFVELRPANAIPLVASLLFGPAAAWGAGIGNVIGDCFGTLGPASFFGFLG
                     NFIFGYLPYVLWGHLGWFSSGQPPLGKSWRQMMEYGVVCMIASAACAGMIAWGVEWLG
                     LLPFVILAPAIFFNNVVMGMFLGPPLLGFLYPRVQRWRLRYEDIRESGASHIHPTKTL
                     EAPDSGSEGTNAHLREAIVDCRGFTFQYASGSAPVLRNVSFSLASGELVVLLGRSGSG
                     KSTICYACNGLIPHMIPGTLSGTMRVKGRDTVDDPVWKQAGRVGLVFQDFDTQLVATT
                     VEGELLHPLEYRDPRLSVDEVRRRVVRALNQIGLADCAHRDPMTMSGGQRQRLVMASV
                     LVQEPALLVLDEPGSDLDPAGRSQLRNVLRNLQQDGITVLMTEHNDDYLAQADRVLVL
                     DEEHIVWEGKPETLLRQPQLMRDCGLRPFVLTECFEEWDLEHVPVSVEEAWTRAEELN
                     LRLAPPAEVLDDTLRLGKLPERESTMALPLIQVDHVSFQYEEQTVLADVSFTIQPGDF
                     LALLGQNGSGKSTLARLLNGLLMPTCGTIVVDGMNTRSTSMHELAQRVGLVFQNPDHQ
                     IFADTVWEEVAFSAKNMGCSEDEIADRVKESLTAVGLPIEGSRDLDPFSLRKGERQRI
                     AVASVLATRPAVLIVDEPTTGLDPLETDRMMAMIRRLNQEGHTIVMITHSMGLVAAYA
                     RRCLFMYSGRIIADGTPREVFADPGLIQSASLEIPAISRFSQRWGQTLLTVEEVNASW
                     RRGFP"
     gene            6528..7280
                     /locus_tag="PKM72_10440"
     CDS             6528..7280
                     /locus_tag="PKM72_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNP61250.1"
                     /translation="MHQLDGRTKVLTVLGVFGLALCFSDPVYLLGVFGGVMGGLTMSQ
                     AGANVRKMWMLTVLLFGYSVALWPFFVDGVTPVPVLHGLGVTWEGVRVGLGMGLRLLI
                     MLWGGIWLLSTTTVEELAHASQQLGLPSRAGFVFSLAFRWVGMFLGAGVGVVQAQRSR
                     GLDVSTGGILQRIRKYVPLVIPLIGHALRQTNLLAMSLESKGFHPSATRHFYATGKLR
                     ARDYTVMSVMLILLGVSLWLRWQGVGTLDMQF"
BASE COUNT         1642 a         1683 c         2197 g         2094 t
ORIGIN      
        1 cctgcgtggg taagatccgg ttgcaagggc tggtgaaggt gggcgatgac gggttgtggg
       61 cggaagaccg ctggaatccg ttgtactatg ccatccgggt ggaacaagtg gcgttgccgt
      121 tgtatccgca atggggcacg gagcccaatg gcttctacat tcccccacgc caagccccac
      181 ggggctacat ccggcagatg ttcgggccgg gagtggataa tgcgattgag aagtatctgg
      241 tgccgagccg ggagttgttg gcggtgctgc agttgtggcg ggcgagccaa cagatcatct
      301 tccggtatga cgtgattccg ggaccgaaag tgtttgaaac ccaaatccat gggcggaagt
      361 ttgagatgta caacgacacg gtgttggggt tcaacaaatc gggcaaggaa gcggtgcggc
      421 agcaagtgga agagccgatt tacatccgcc cggcggagcg ggtgaactgg ctgtaagcgg
      481 aagcccagag gggagaccga agagcagggg gcgacagatg tcgccccctg ctttttttta
      541 ttcgaaattc ttcaagcatt tttacaatgc actttagaac tgtaaatccg tattagctga
      601 aaatgttttt tgataccgtc cggtctttac ctttttgctc gacaaatgag gcatgcatag
      661 gattaagaaa aaacaggggt tccttattca ggaaaaaatc tacatgtagg gacaaagact
      721 gtatctcatg atggggaaag gaggtgagtt cggagaacgc acgggattcc actgtatccg
      781 cattgtacgg cgttgaaaca tgtgcaaagg atgaaattgg atgaaaaata aatgatgatt
      841 ttattggatt ttattgtaac tatagctcat aaattgtcta tgttcagagc acctctccta
      901 ttggtggaat tatagagttt cattggaaga gtgtttgaaa cttttttttc ccgctcatca
      961 accaaacgat tctatgggtt gcttggagag gacattcttc tttgaatgcc ataatggcct
     1021 gggcttttga acttttcgac cccgagacca gtccggtata gttaatcttt tgataaaatt
     1081 gggtgaaaat tacaagctct gttgtattga ttctttttgt catgtctggc tggataagga
     1141 gaggtcctaa tgatgcaatt ggccaaattg aaaagtgaat tagacgatgc ccggctctgc
     1201 acggatcgtc tcttctccct tgtgagtgat gaagccatgt atgagcgtcc tatccccgag
     1261 cgacatcgtt taatttttta cctgggccat cttgaggcat ttgactggaa tcagattggt
     1321 cgttggacat tgggcatgcc gtcgtttcat gaatcatttg accagttatt tgaagcggga
     1381 attgatcctt ccttgggaaa tctgcccgcc gatcgaccat cggattggcc gacaatacat
     1441 gaagtgcacc gttataatgc acgtgctcgt gaagaagtgg atcgcctctt gccaactgtt
     1501 ccggaatgta tcatccatat ggcagtggaa catcgtctga tgcatgccga gacgaatgcc
     1561 tacttactcc atcatttgga ttcaaagtac aagcatgtgc cagcgaattc ctccgggggc
     1621 ttatcttctt cattttcaga agacatacct aaaaatgaga tgatggaagt gccagaaggc
     1681 atggccaccc taggacggga gccagaagag gggtttggct gggacaacga attcttgcaa
     1741 catgtcgtcc ctgttcctgc gtttttgatc agccggcata aagtgacgaa ccaacaatat
     1801 ctgcgatttg ttcaggaagg tggcgagccg tccgcatttt gggtcaaacg aggggatgcc
     1861 tggtatgtgc gcacgatgtt ccaggaagtc ccattaccgc cattctggcc ggtgtatgtg
     1921 acacatcgac aggcggaggc ttacgctaat tgggccggca tgaccctccc gaccgaaccc
     1981 caatttcatc gcgccgcctt tggcactccg tccggtattg agcaatcctt tccctggggt
     2041 aatgcagcgt caatgtttca acatgggaat gttgattttc atgcttggga tctcgctcct
     2101 gtgaatgccc atcctcttgg ggacagtgcg tttggtttgg ctcagatggt gggaaatggc
     2161 tgggaatgga catcgaccgt tttccatcct tttcatggat tttcaccctt tcccacgtat
     2221 cctggttatt cagccagatt ttttgatcag gatcattatg tggtgaaagg tgccgggcct
     2281 tttacctcca gccgactctt gcgccggtcg tttcgaaatt ggtttcgaca aagttacccc
     2341 tatgcgcata tcggtttccg ctgtgtgcag tcataatgct catgggtgac gctgcttctt
     2401 ttccgtgttg catatcggat gacacctttt tatgcgactg atacttaaaa gacgggggca
     2461 ttgggggatc ctcctttttg tcttgattct ctgggggaac ccagcctttg cgaccgaccg
     2521 gttggtgatt ctgcattcga gcgagcatca cggggttgcg cttccgcttg accaagccgg
     2581 agagacgaga gttggaggcc tggccaggcg ggccacaatt gcggaggagg ttcggcggga
     2641 aggcgtgccg gtcttgatgg tggattcggg ggatatcctt attggaaccg ctatgtcttc
     2701 ctggtttcgc ggtgaaccgg atattcgggc catgaatctg atccgctacg atgcgatggt
     2761 ggctggaaat catgattttg attatggaat tgaccacctt cgcacattgg ttgagttagc
     2821 cgatttccca attttatgca cgaaccttca gtccaagcga tctgtcctcc cttgccggcg
     2881 ctcagttgtc acccgcttgg ggaatgtgag tgttggaatc gtcggaattg tggggaacag
     2941 taattttcct gataccttca accgggatgt cgccaatacg ttatcattga tggatccgat
     3001 tgcctctctt caaactgaag ccctgcggtt acggacagaa gcccatattg acctggtcat
     3061 tgccctcaca catcaagata gcaatgaaga tttaaaaatt ctggagaccg ttgagggggt
     3121 ggatgtgctc atcggtggac ataccgaagg gtttgacgga ttgtatgctc ctggtttgat
     3181 ggagccggtt gaatccgtga ctcgtcctcg atcggtgtat gtgaagacgc atcggcaggg
     3241 ccgaacagtc gggcgattag atctgacgat tgaggaagga tcggtgatcc aggcgcgttc
     3301 acacaatatt ccagtgaccg tggaagttcc agtcgagccg aatgtccaac aacttctgag
     3361 cgagtatcga cagcggtttg cccgtcaagc cactcagata ttgggggagg cgtctgtgcg
     3421 gctccagggc gatcggcctg ttgtccggac ccaagaaacc aatttgggaa atttgttggc
     3481 agatcgtatg cgaaggaaat ttgagacgga tatcgcctta ataaattccg gacaaattcg
     3541 tcgaagcctg gaacccggtc cggtaacatt aggcgatatg ctttcggtcc tgccctttga
     3601 ctccgccctt gtgaccgtac atgtgacggg agcgatgctt cgtcaggttt tagagcatag
     3661 tgtgagtcaa tggccgaatc attccggtcg gttcctccag gtatccggtc tccaggtcac
     3721 gtatcaagga aaggccccgg ttggatctcg tgtgaagagc atcatggttg ggggtatgcc
     3781 cttagatatg tcaaaaacct atactgtcgc gacggacgcc tttgtggctg agggcgggga
     3841 tggctatgac atgttgactc acctctcgga tcgaagggac catcaaatcc ccttgagaga
     3901 tctgttgttg aaagccttag ctgatggtcc gctctatgcg gaagcagatg atcgtatcgt
     3961 attcgtgaat agcttcccgt ctcattaacc tctcatcact gtatgtctct tccccaagct
     4021 gctcgtgatt cacgacgatg gtgggctgat ttggtagctg tctggcggga tacccggcag
     4081 attgttctaa cggctcagat tgcggcaatt tatgccgcaa ttcttattcc ctttaaagcc
     4141 ggcattccga ttgttcctgg ttttgtggaa ttacgaccgg ctaatgccat tcctcttgtg
     4201 gcatcgctgt tgtttggtcc cgctgcggct tggggcgcag gtattggcaa tgtcatcgga
     4261 gattgtttcg gcaccttggg gccggcaagt ttctttgggt ttttaggcaa ttttattttc
     4321 ggatatcttc cgtatgtatt atgggggcat ttggggtggt tctcatccgg ccagccgcct
     4381 ttggggaaat cttggcgtca gatgatggaa tatggcgtgg tatgtatgat cgcttcagcc
     4441 gcctgtgccg gaatgattgc gtggggagtg gagtggttgg ggttgttacc ctttgttatc
     4501 ctcgctccag cgatcttttt taataatgtg gtgatgggca tgtttctcgg tcctccatta
     4561 ttggggtttc tctatccgag agtgcaacgg tggcgcttgc ggtatgaaga tattcgagaa
     4621 tcaggtgctt cccatattca ccctaccaaa acgctggagg cacctgattc ggggagcgag
     4681 gggacgaatg ctcatttgcg cgaagcaatt gtggactgtc gtggctttac tttccagtat
     4741 gcgtccggtt cagcgcctgt gctcaggaat gtgtcatttt ctctggcttc cggtgaactc
     4801 gttgttctgt taggcaggag tggaagtggg aagtccacga tatgctatgc ctgtaatggt
     4861 ctcattcctc atatgatccc tgggacactt tccggaacga tgcgagtgaa gggccgcgac
     4921 accgtggatg atcccgtgtg gaaacaggct gggcgggtcg gcttggtctt tcaggatttt
     4981 gatacgcaac ttgtggcgac gactgttgag ggggaattgc tccacccatt ggaatatcgt
     5041 gaccctcgac tttctgtgga cgaggtcaga cggcgggtcg ttcgtgccct caatcaaatc
     5101 gggttggccg attgtgccca ccgtgatcct atgacgatgt ccggtggcca gaggcaacgt
     5161 ctggtaatgg cttctgtgct ggtacaagaa ccggcgctat tggtcctgga tgaacccggt
     5221 tcagaccttg atccggcagg ccggtcacaa ttacgtaatg ttttacggaa tcttcaacaa
     5281 gacggaatta ctgtgctcat gaccgagcat aatgatgact atcttgcgca agcggatcga
     5341 gttctggtct tggatgagga gcacatcgtc tgggaaggaa agcctgagac cttgttgcga
     5401 cagcctcagt tgatgcggga ttgtggtctt cgacctttcg tcctgacaga atgttttgaa
     5461 gaatgggacc tggaacacgt gcccgtttcc gtcgaggaag catggacccg ggctgaggaa
     5521 ttgaatcttc gtctggcacc tccggcggag gttcttgatg atacacttcg gttggggaag
     5581 ctgccggaga gagaatcaac gatggctttg cccttgattc aggttgacca cgtttctttc
     5641 cagtatgaag agcagacggt cttggctgac gtgtcattta ccatccagcc aggagacttt
     5701 ctggcattgc tgggtcagaa tggatcaggg aaaagtacgc ttgcacgatt gctcaatggc
     5761 ctcttgatgc cgacatgcgg caccattgtt gtggacggca tgaatacccg cagcacctcg
     5821 atgcatgaat tggcccaacg cgtcggatta gtttttcaaa atcctgatca tcaaattttt
     5881 gcggataccg tctgggaaga agttgccttc agcgcgaaga atatgggttg ctcagaggat
     5941 gagattgccg atcgggtgaa agaatcgttg actgctgtgg gattgcctat tgagggaagt
     6001 cgggacttag atccgttttc cttaagaaaa ggggagcgac agcggattgc ggtggcgtcc
     6061 gtgttggcta cgagaccggc tgtgttgatt gttgatgaac ccacgacagg gcttgatccc
     6121 cttgaaactg accggatgat ggcgatgatt cggcgactga atcaagaggg gcatacgatt
     6181 gtgatgatca cacattcgat ggggcttgtt gcggcttatg cccggcgctg tctattcatg
     6241 tacagcggga ggattattgc tgatggaacg cctcgagaag tttttgctga tcccgggttg
     6301 atccagtcag catcattaga gatcccggct atttcccgct ttagccaacg atggggacag
     6361 accttactaa cggtggaaga agtgaacgct tcctggaggc gaggttttcc atgagcattg
     6421 ttaccgatac gaatccggta atggaaaacg tgtgtggcat gtgtgagatt catagagcct
     6481 ggtaaatacc gatgaacgtt tccttgtatt tatccaggaa atcgtggatg caccagttag
     6541 atggtcgcac caaagttctg acggtgcttg gggtgtttgg attagccctg tgtttttcgg
     6601 acccggttta cttgcttgga gtatttggag gcgtgatggg tgggttaact atgtctcagg
     6661 caggggcgaa tgtcaggaaa atgtggatgc tgacggttct cctgtttggc tatagcgtgg
     6721 ctctctggcc attctttgtc gatggtgtga cccctgttcc cgtcttgcat ggccttggag
     6781 tgacctggga aggtgtcagg gttggtttag gaatgggtct ccggctgttg attatgctct
     6841 ggggtggtat ttggttactc tcgacaacga cggtggaaga gctcgcgcac gcctctcagc
     6901 aattgggact tccttcacgg gcagggtttg tcttctcact cgcgttccgg tgggtgggaa
     6961 tgttcttagg agcaggcgtg ggtgtggttc aagcgcagcg atcacgcgga ttagatgtgt
     7021 cgaccggcgg aattctacaa cgcattcgta aatatgttcc cctggtgatt cccttgattg
     7081 ggcatgccct tcgtcaaacg aatcttctgg cgatgtcgct ggagtctaaa gggtttcatc
     7141 cctcagcgac acggcatttt tacgctaccg ggaaattgag ggcccgggat tacacggtca
     7201 tgagtgtgat gctgattctt ttaggagtaa gcctttggtt gcgatggcag ggcgtcggta
     7261 ctctcgatat gcaattttga gaatttgatg cctgcatatt ttcgctcaaa tctaaagaca
     7321 atcccaaaaa tcctcttttc cattgtggga tgccgaaagc gtcggttgga tgatgtggac
     7381 aatggaaaga ttggctcagc tagaagctta aggccaatgg ctctggttgc gagttccgat
     7441 gaggtacctg agaaggacag attgtgaagc tggtgtgagg tggtaggtag ggaaatccta
     7501 acttatcatt ggggtctgtc ggcaggatgt ctcttctgtc gcgagtgcga caaattttga
     7561 aatctttttg atgcaacggc cacagcactg ccgtttgtca ttgagttcga gggcgt
//
LOCUS       DAORAQ010000125         7594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1507_length_7594_cov_62.352699, whole genome shotgun sequence.
ACCESSION   DAORAQ010000125 DAORAQ010000000
VERSION     DAORAQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1507_length_7594_cov_62.352699"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1509
                     /locus_tag="PKM72_10445"
     CDS             178..1509
                     /locus_tag="PKM72_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61251.1"
                     /translation="MMHWKKIIVLLSCLVLSLGALQSWAHPFPETAGDQDAQPSDRSL
                     SSLVQKYVDAAPDRAKELLADIQQRPDATVESISKILQQPRQYETQPVGAQPRRPIRV
                     RGEDAEYALYVPPSYSPEKPMALILCLHGAGFTGEAYLDRWIPRLGEKYILACPSVTM
                     GSWWTRFGEDLALTVIAEVQTQYHIDPDRIFLTGMSNGGIGTWIIGMHYADRFAGIAP
                     MASGIDDVLFPFVENLVHTPVYVIHGAADRVMPVQLSRDLVKEMERRGISYQYQEHTW
                     THPHAGGHFFPKQALPELITWFDSQQRKPLPRTISLVRDATHLTPFSWVRIDMTDQIA
                     AFTENLIDSRDEFITGGVYAKLHAQVTDSNKFEVSTNRIRRYTLFLNQDLVDFSKPVI
                     VETNGKVSYEGMVEPRIDTLLQEVRHRSDFHTLFPVKLTIDVPSSDIVKEK"
     gene            complement(1678..3438)
                     /locus_tag="PKM72_10450"
     CDS             complement(1678..3438)
                     /locus_tag="PKM72_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HNP61252.1"
                     /translation="MNRKFQNKKIVPAWTRSVLAGSVLIALIGTLLPETFAQENRPNA
                     GRDNHDRRPGANRDNPETKPDHGWNNLEIRAIDGRNNNVQNPDWGSANSQLLRQVKAD
                     YENGSWAPAGYNRPNVRDISNTVAAQDKPIFNEKSASDFIWQWGQFIDHDIDLTEGAD
                     PLEPFHIQIPTGDPHFDPQSTGTQHMSLNRSAYDPATGTDSRNPRQQMNQNTAFIDAS
                     MVYGSDPVRAAALRANDGTGRLKTSPGNLLPFNTQGLPNQGGPAPTLFVAGDVRANEQ
                     VGLTAMHTLFMREHNRLADLMHANSPRLSEDAIYEAARIWVGALIQVITYKEFLPVLL
                     GPNALRPYTGYKRGVNPDIGNEFSTAAYRVGHTLLSPVLLRLGKDGKSIREGHLPLRE
                     AFFAPGRLINEGGIDPLLRGLANNRAQRVDNYIIDDVRGFLFGLPGEGGFDLVSLNLQ
                     RGRDHGLASYNDARLAFGLSPAVSFDDITKNREVQERLEAAYGSVELVDLWMGGVSED
                     HRPGALVGNLFYTILVDQFERLRDGDRFFYLNGFPTFEIKKLEETTLADIIRRNTSID
                     REIQDNVFLVSQKDNRQRRR"
     gene            complement(3846..4775)
                     /locus_tag="PKM72_10455"
     CDS             complement(3846..4775)
                     /locus_tag="PKM72_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61253.1"
                     /translation="MPNTLAHIGVQGLLNQAVKPGIDPKLVCMGCLLPDIPWIFQRVL
                     MGLVPGSDPYTIRLYCIAQASLFVTLILCGALAFLTRTPRAVFLILGVNVLLHLVLDA
                     LQTKWANGVHLFAPLSWDLWNVGLFWPESLPTYIMTAGGLFYIGWQWRKGISQPLPIS
                     LLSPFKLAISASLLILYFALPFVLLEGPRNANNHFVRTLEETAARPGRLLELDRPSYV
                     KTPSGDMIGLFNGEQIGTTQQILDHSATVSVRGRFVTPTQLDVLEFHEHSSWFRDTGS
                     YLGILLLFGMWLGAFLKQGMFPYSHKFKKFPNT"
     gene            5298..6185
                     /locus_tag="PKM72_10460"
     CDS             5298..6185
                     /locus_tag="PKM72_10460"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNP61254.1"
                     /translation="MRVELSEVGIMTDSKAMRRNAIVNGVSIGIPFIGTLIGLLSSGF
                     SAPTSSTVFLFLLFFLFNLIGVSVGLHRYFSHHAFETSTWVRWLLGILSTWAMQGPID
                     RWVADHRRHHRFSDQPLDIHSPYWSRQNKITSGFMRFAHAHLLWMFVEWPTDTRLYAK
                     DTISDPISRRCSRWYWPICATSFLVPAMLGYAMGGSDEAVRAFFWAGCLRIFLMHQFT
                     WMVNSFGHMFGGKTEGSRDESRNMHWGVTLLFLGEGLHSYHHAHQRTAVNQPASLDPA
                     GYLITFLETIHVVWKVQRF"
     gene            complement(6377..>7594)
                     /locus_tag="PKM72_10465"
     CDS             complement(6377..>7594)
                     /locus_tag="PKM72_10465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNP61255.1"
                     /translation="MAGGCLVIQGPPGTGKTFTASQVITRLLASGKKVGVASNSHKAV
                     INLLTACGDAVKGSGSQLHGLKVGGDPDDPLFSCNPGVQYLKDTGTAHQAYTRGVVGG
                     TAWLFTRPEWEGALDFLFIDEAGQVALANAMAMARCAKNLVLLGDQMQLEQPVQGSHP
                     GDAGLSVLQYALKDMEASQPDSPIMHAVVPPDYGLFLGKSRRMHPAVCRFISESMYEG
                     RLGSHPDCARQRIIVPPGANSLITCESGILFSGVEHDGNIQQSDEEVERVTTMYHELH
                     GRLYTDKDGITKPLDLDHFLFIAPYNAQVRALQAALPDGARVGSVDKFQGQEAPVCIL
                     SLCSSYGEYGSRGLAFILDRNRVNVAISRAQCLAVVIADPRIAGTPPGSLEEMKLINL
                     FCKLTMYPPPCAL"
BASE COUNT         1762 a         1868 c         2059 g         1905 t
ORIGIN      
        1 aatatttctt ctacaaaaat cccgcataat tcccgtttcc ttctacattg aagctttcag
       61 aattttaccg tcgactacgc ctctgaccct ataggaatgt ccggttgaat atgagaggat
      121 aattgaacgg tcattaatct gactgttctc atggtcaaaa aagtcaggga aatgttcatg
      181 atgcactgga aaaaaattat tgtccttctc tcatgtctgg tactgagcct gggggctcta
      241 cagagttggg ctcatccatt tccggaaaca gcaggagacc aggacgcaca gccttctgat
      301 cggagtctgt cctcattggt gcaaaaatat gtggacgccg ctccggatag agccaaagag
      361 ctgttggctg acattcaaca gcgccccgac gccacggttg aatccatttc aaaaattctt
      421 cagcagcccc gccaatatga gactcaaccg gttggtgccc aacccagacg accaatacga
      481 gtgaggggtg aggatgccga gtatgccttg tatgttcctc cttcttattc acccgagaag
      541 cctatggccc tcattctctg tttgcatggg gcagggttta cgggggaagc ttatctggat
      601 cgctggattc cacgtctggg tgaaaaatac attctggctt gtccgtcggt aacaatgggg
      661 tcctggtgga cccgattcgg cgaggatctg gcgttaacgg tcatagctga ggttcaaacg
      721 caataccata tcgatcctga tcggatcttt ctcaccggca tgtccaatgg tggtattgga
      781 acctggatta tcgggatgca ttatgcggat cgttttgcgg gtattgcgcc gatggccagc
      841 ggtattgatg atgtgctgtt tcccttcgtc gaaaacctgg tccacacgcc cgtctatgtg
      901 attcacggtg cggcggatag ggtcatgccc gtgcagttga gtcgcgactt ggtgaaagag
      961 atggagcgtc gcggcatttc ttatcagtac caggagcata cctggacgca tccccatgcg
     1021 ggagggcatt tctttcccaa gcaagccctg ccggaattga tcacctggtt cgatagtcaa
     1081 caacgaaagc cattgccacg gaccatttca ttggttcggg atgccaccca tctgacgccc
     1141 ttctcgtggg tgcgtatcga tatgacggat cagattgccg cctttacgga aaacctgatt
     1201 gacagccgtg atgaatttat cacgggaggg gtatacgcca aattgcacgc ccaagtcaca
     1261 gattccaaca aatttgaggt gagtaccaat cggattcgcc gctataccct gttcctgaat
     1321 caggacctgg tggatttttc caaacccgtc atcgtggaaa cgaatgggaa ggtatcctat
     1381 gaaggcatgg tcgagccacg catcgacacg cttttacagg aagtccggca ccgctcagac
     1441 ttccacacac tctttcctgt caaactcacc attgatgtgc cctcttccga tatcgtgaaa
     1501 gagaagtagt ggtctgatca aaaatctacc cacctgcgcg ggaaccgaaa aggtggatga
     1561 tgacaaaagg caatctgaaa cacccccaaa atgaatagca gaaaacccgg gtgctgccca
     1621 tttgagcttc ctgccagggt tgattcaaaa tctagatcgt ggtcttgagg aaagggctta
     1681 gcgtcttctc tgacgattgt ctttttgcga gacgagaaac acgttatctt gaatctcccg
     1741 gtcgatggaa gtattccgcc tgatgatgtc ggctagggtg gtctcttcca attttttaat
     1801 ttcaaaagta gggaacccat tgagataaaa aaaccggtct ccgtcccgca accgttcgaa
     1861 ctgatccacc agaatcgtat aaaataaatt ccccactaac gcgccagggc gatgatcttc
     1921 agacaccccg cccatccata aatccacaag ttcgacgctt ccatatgcag cttccagacg
     1981 ttcttgtact tcacgattct tggtgatgtc gtcaaaggac acggcaggtg agaggccaaa
     2041 ggccaaccgg gcatcattgt agctcgccag tccgtggtcc cgtccccgtt gaagattgag
     2101 tgagaccagg tcgaatcctc cttctccagg gaggccgaag aggaaacccc ttacgtcgtc
     2161 gatgatatag ttgtcgaccc tttgcgcgcg attgttcgcg agccctcgaa gtaacgggtc
     2221 aatccctcct tcattgatga gacgccctgg agcaaaaaat gcctctcgca gcggtaaatg
     2281 gccttcccga atcgactttc catctttacc cagtcggagt aataccggtg agagcagggt
     2341 atggcccaca cgataggcag ccgtactgaa ctcattgcca atatcgggat tgaccccacg
     2401 cttgtacccg gtgtagggcc tcagggcatt gggtccgagg agtacgggga gaaattcctt
     2461 ataggtgatg acctggatca acgccccaac ccagatcctc gcggcttcgt aaatggcatc
     2521 ctcgcttaat cggggggaat tggcatgcat gaggtcggcc aggcgattat gttcccgcat
     2581 gaacaacgta tgcatggccg tcagccccac ttgctcattg gctcgcacat ctcccgctac
     2641 aaatagcgtg ggagcgggac cgccctggtt aggcaggcct tgggtattga acggcagcaa
     2701 attgcccggg ctggttttca gccggcctgt tccgtcattg gctctaaggg ccgcggctcg
     2761 taccgggtct gagccataca ccatcgatgc gtcgataaac gctgtatttt gattcatttg
     2821 ctgccgtgga ttccgggaat ccgtcccggt agccggatca taggcggacc ggttcagcga
     2881 catgtgctgt gttccggttg attgtggatc gaagtgagga tcgcctgtcg ggatttgaat
     2941 atggaagggt tcgagggggt ctgccccctc ggtcaggtcg atatcgtggt cgatgaattg
     3001 tccccattgc cagataaagt ccgaggccga tttttcgttg aagatcggtt tgtcttgggc
     3061 ggccaccgta ttggaaatgt cacggacatt tggccggttg tagccggcag gtgcccagct
     3121 accattttcg taatccgctt taacctgccg aagcaattgg ctattggcgc ttccccagtc
     3181 ggggttttgc acattattgt tcctgccatc aatggctctg atctcgagat tgttccagcc
     3241 atgatcgggt ttggtctcgg gattgtccct gttagcaccg ggtctgcggt cgtgattgtc
     3301 cctgccggca ttgggtctgt tctcttgtgc gaacgtctcg ggtaagaggg ttcctatgag
     3361 agcgatcaaa acgctgccgg ccaaaacgga tcgtgtccat gccgggacta tttttttgtt
     3421 ctggaatttg cgattcacgt actttttctc tcgaatttcc taatggtatg acttatggtt
     3481 gtaacccttt gggaaactcg ggtagaaaaa gatgaattta gaaaaacgat gaagccggga
     3541 tgaaaaaacc cggcgatgat gaataaatcc atgggaaaaa gaaatttacc ccagtcgtgg
     3601 aaataaatcc agtagaggaa aaactagttc ggaggtttaa actcagagaa gtgatggggt
     3661 gattcggcgg gtgaaaaatg gaaattagtg aggggggttt taaaaaatta tggctggttt
     3721 caaaacttcg accgaaatca ggcctggctt gggggaatat ccccgtttgg ctggcagttg
     3781 gatgaccttt ctcgccttcc tacttagcgg tttcccccct ggatggtttg ggtatcaacg
     3841 gtttgttacg tgtttggaaa ttttttgaat ttgtgtgaat aggggaacat gccttgtttc
     3901 aggaatgccc ccaaccacat gccaaacagg agaaggatac ccaggtaact acccgtatca
     3961 cggaaccaac tggaatgttc atgaaattca agaacgtcca gttgggtggg tgtgacaaac
     4021 cgcccacgaa ccgagacggt agcggagtga tccagaatct gttgcgtggt gccgatttgt
     4081 tcaccattga ataggccaat catatctcct gagggagttt tcacatagga tggccgatcc
     4141 agttccagaa gacgtcctgg acgagccgcc gtttcctcaa gtgttcggac gaaatggtta
     4201 ttggcgtttc gaggcccttc taacaggaca aaaggcagtg cgaaatagag tatgagtaac
     4261 gacgccgata tcgccaattt gaacggagac aggagcgaaa ttgggagagg ctgtgatatc
     4321 ccctttcgcc attgccatcc gatatagaat aagccacccg cggtcatgat ataggttgga
     4381 agactttccg gccaaaataa ccccacattc cataagtccc atgacagtgg agcaaagaga
     4441 tggacgccat tggcccattt ggtttgaagc gcatcaagca cgagatgtag gagaacgttg
     4501 accccgagaa tcaggaacac ggctcttggc gtacgcgtaa gaaaggccaa ggccccgcat
     4561 aagatcaacg tgacgaataa cgaggcctgg gcgatacaat acaggcgaat ggtataggga
     4621 tcgcttcccg gcaccaatcc catcaagact ctttggaaaa tccaggggat gtccggaagc
     4681 aaacaaccca tgcagaccag tttgggatcg attccgggtt tgaccgcctg attcagaagg
     4741 ccttgcacgc cgatgtgggc taaggtattg ggcacgactt cctttcacca gaaattaggt
     4801 aaggtatcgg caaaatgttt gcccaccata atcggcttca ctagggattc tcaaggaggg
     4861 tgtttctcag gattcattta tcgtggaaac gaatgggaag ttttctttcg aggacatggt
     4921 cgggtctcgc attgacacgc ttctcccgga agtccggcac cgctctgaaa cccgcagcct
     4981 ttttttcgtc aagattacca ttcaggtgcc agagcctccg gttgtttaat accaccgttc
     5041 acttgttgtt tcgaatgagc cattcctgtg gggcatatcc tttttaggct catgccccct
     5101 tgtcagacgc cctcgttcag ttaaaatttc cttacactcc ccaacccatt gtcttgaggc
     5161 aaaaggctgg tgcatgttcc ggttggtttt ctccttttga cgtcaaaacg ccaatggcgt
     5221 tcttgtaata ttggataaaa ttttgaggtg aatgagagac acaaacacat gttttgtgag
     5281 gaaggtagga gtttctgatg agggtggaat tgagtgaagt gggcattatg acagattcaa
     5341 aggccatgag gcgcaatgct atcgtgaatg gggtgtcgat tggaatccct tttattggca
     5401 cgcttatcgg gctcctgtct tctggtttct ctgctcccac ttcctccacg gtttttctct
     5461 tcctgttgtt tttcctgttc aaccttatcg gggtgagcgt tgggttgcat cgatatttct
     5521 ctcatcatgc cttcgaaacg agtacatggg ttcgatggct attgggtatt ttgagcacct
     5581 gggcgatgca aggtcctatt gatcgatggg tggctgacca ccggcggcat catcgttttt
     5641 ctgatcagcc attggatatt catagtccgt attggagtcg gcaaaataag atcacctctg
     5701 ggttcatgcg tttcgcgcat gctcatctat tatggatgtt tgtggaatgg ccaaccgata
     5761 cccgtctcta tgcgaaagat acgatcagtg atccgatcag tcgaagatgc agtcggtggt
     5821 attggccgat ctgtgccaca tcgtttttgg tgccggccat gcttgggtat gccatgggag
     5881 gttcggatga agcggttcgc gctttcttct gggccgggtg tttgagaata ttcctcatgc
     5941 accaatttac ctggatggtt aattcttttg gtcatatgtt cggtgggaaa acggaaggca
     6001 gccgggatga atcgcgaaat atgcattggg gagtcacgct tctctttttg ggtgaagggc
     6061 tacatagcta ccatcacgcc catcagcgca ccgctgtgaa ccaaccggcg tctttggatc
     6121 cggccggata cctcatcacc tttcttgaaa ccattcatgt cgtctggaaa gttcaacgct
     6181 tttaaatccc agccggttct gatcgcggga aacgggtggc agcgttgaag cttatccaga
     6241 atttaggatt ccagtcttcc agcggtatgt aggcttccgg caagggtaca gccctcgcgc
     6301 aatctgcgac gggtctcaaa cgcttacatc agaaatgggg gaactcccgt atgggcctcg
     6361 ttgaggggcg gagcgatcat aatgcacatg gaggtggata catggtcaat ttgcaaaaca
     6421 aattgatgag tttcatctcc tccagggaac cgggaggagt gccggcgatt cgaggatcag
     6481 caatgaccac ggcaagacat tgagcccggg agatggccac attcacccgg ttgcgatcga
     6541 gaatgaaggc cagtccgcgt gaaccgtatt caccgtaact ggagcagaga gaaagaatgc
     6601 agacgggagc ttcctgccct tgaaatttgt caacgcttcc aacgcgtgcg ccgtcgggta
     6661 gcgcggcctg aagcgcgcgg acttgagcgt tgtagggggc gatgaaaaga aagtgatcca
     6721 agtctaatgg tttggtgatg ccatccttgt cggtgtaaag acgtccatgg agttcgtggt
     6781 acattgtcgt gacccgttca acctcttcat cgctctgttg gatattgcca tcatgctcca
     6841 ccccactgaa gaggatcccg ctttcgcagg tgatgaggct gttggcacct ggtgggacaa
     6901 tgatccgctg ccgggcgcag tcgggatgtg atccaaggcg gccctcatac atgctttccg
     6961 agatgaaacg gcagacggcc ggatgcatcc ggcgggactt cccgaggaaa agcccgtagt
     7021 ccggaggtac cacggcatgc atgatgggag agtcgggttg gctggcctcc atgtccttga
     7081 gcgcgtactg gaggacggaa agtcccgcat cgccgggatg ggagccttgc acgggttgtt
     7141 cgagttgcat ctgatcacct agaagcacaa ggttcttcgc gcatcgagcc atcgccatgg
     7201 cgttggccag ggccacctgt ccggcttcat caatgaacag gaaatcaagt gcgccttccc
     7261 actcaggcct ggtaaaaagc caagccgttc cgccgaccac gccacgggtg taagcctggt
     7321 gcgcggtgcc ggtgtctttg aggtattgca cgccggggtt gcagctaaac aagggatcat
     7381 cggggtcacc accaacttta agtccgtgga gctgggaacc actcccttta acagcgtccc
     7441 cgcaggcagt gagaagattg atcacggcct tgtggctgtt tgatgcgacg ccgactttct
     7501 tgccggaagc gagcaatctg gtgatgacct gtgaagccgt gaatgttttt cccgtgccgg
     7561 gaggtccctg aataaccagg catccaccgg ccat
//
LOCUS       DAORAQ010000126         7566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1511_length_7566_cov_66.747703, whole genome shotgun sequence.
ACCESSION   DAORAQ010000126 DAORAQ010000000
VERSION     DAORAQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1511_length_7566_cov_66.747703"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKM72_10470"
     CDS             <1..282
                     /locus_tag="PKM72_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61256.1"
                     /translation="QMDPKDETMWFGLGKAYMSDENWKEAIPALEQCILVKPEYSAAY
                     FALAQSLHQTREFEKCQTICTEGITVATKNGDLLVIKNLEALKASLPSS"
     gene            complement(322..1092)
                     /locus_tag="PKM72_10475"
     CDS             complement(322..1092)
                     /locus_tag="PKM72_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61257.1"
                     /translation="MDVNLGHPQKDLIIRQWQELAQACPDGESQARLAMLWDHISRFP
                     VRQAASAYAEIETAYFLAQAGFSVAFLEASGGRTADLECYEGTHRFFVEVTVIQSTQG
                     ATRKSPAVRLQPHQILESSDEFFEQALVKRLLSRMAEKARQLERYCAPVLLAVSVPDL
                     PWGKGRPQDIPPLDLQRLAAMLVGVVVDVPQFSAILLTLWKAPAQELRNPIRIRQVTW
                     VTRPPGNPRDPRIRLLAVNPVARYRLSARELKSIQEEM"
     gene            complement(1307..1990)
                     /locus_tag="PKM72_10480"
     CDS             complement(1307..1990)
                     /locus_tag="PKM72_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61258.1"
                     /translation="MSTLPGSTDEKWVIYWEREYSTAFAPERLKLDFHPHRPLMTNMP
                     LSEQREVAASGYKVIPDDCGPVRFAGQYGWLIRSPADCRIRKKGSGFEWQSPPIRPEE
                     QLLGYKSFSGMYVDTILNSGFPKLYCGIRFYYPMELGMMMKDLPNHFYYFPKRTFSIW
                     EGIKTQEYKVTPDDFLPGYAAFVTNFLIQLDSSTEEPTTILRGDPIGLVLPVLLPKHF
                     TLQELKRTP"
     gene            complement(2001..2648)
                     /locus_tag="PKM72_10485"
     CDS             complement(2001..2648)
                     /locus_tag="PKM72_10485"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNP61259.1"
                     /translation="MKVFRGNILWVSVFAVVWWCGCVQVFAQPSSPHTPPQASHGLQV
                     LVTYHSLSGHTAEMAKAVKKGAESVKGTTVLLKTVGQVTADELFGSDAVIVGSPVYWS
                     NMSGEVKSFFDRWQFEFGVFPEWKMRDKVGAAFATGGQVSSGKEVTMLTILAAMLGNK
                     MIVVSDGGAFGASATTEGDSPGINDVERADAWALGKRVAEVAGMIKRGQLLPGQP"
     gene            complement(2724..2909)
                     /locus_tag="PKM72_10490"
     CDS             complement(2724..2909)
                     /locus_tag="PKM72_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61260.1"
                     /translation="MKYRKQVHYVKGVFACPKCHQHFVQEKWLEEFRVRCHKCDYRGA
                     LTHVSVEEQIDEEIERR"
     gene            complement(3115..4044)
                     /gene="xerD"
                     /locus_tag="PKM72_10495"
     CDS             complement(3115..4044)
                     /gene="xerD"
                     /locus_tag="PKM72_10495"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNP61261.1"
                     /translation="MREFVDRYLTMLQVEQGYAVNTVESYRRDLRKLEVFFRTHDIAD
                     PTHLSKPLWFQFLSSLKGEGLSSSSIARCLASVRGFYKSFERGKDDPTFDGMLKGTPK
                     QWSQLPKLLSEAEVTRLLNLPIMATREDSRDAAMVELLYATGLRVSELINLEMAHLNL
                     DVGFLQATGKRDKQRIVPIGDKARQLVSAYLQSSRPAFVKKRTSSALFLTRLGRAMSR
                     QCFWKILKDRTARAGITKPISPHMLRHSFATHLLDHGADLRSVQMMLGHASIATTQIY
                     THVEQARLKKVHDQYFPRKQRTRVIDAPNGSES"
     gene            4364..6244
                     /locus_tag="PKM72_10500"
     CDS             4364..6244
                     /locus_tag="PKM72_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNP61262.1"
                     /translation="MGSLLDQGWWPFMGGGTNLTFAQQASISPLLDQAKLELEADNPA
                     AATTTLEQLIDSFPPPEILEEAYLLQATALKHDHQPTDALTVLKQLLEEFPFSRSTNP
                     ARVLMAELYIELEDYRPALDQLYQALDYATELDLRRSIFQLIRQTELANHNPLGAVKA
                     LLNEMSLADQTERLELEGLAQSLILQQLDEPALQELVESYASRYPGDIATIRLIELHT
                     AHGDEVLAERDIRGFLKRFPTHPYAQTAMALLQSFISKIKFHSHIVAAALPFSGPMKN
                     YGTDSLNGIRLALEIAKEQWGLTAVGLVVKDTTTLTAPLRVEIQQMLDEFQPVAVVGP
                     LLSREIQELSRLPDENAIPFVTPSSTLLNVRQFGSFWFSTAMTSSVQAKRLVEYAILK
                     LGYHRFGIIYPKTAYGREMADMFAKEVLHSGGEIIASEGYADDQTDIGEQLRHLKEKD
                     LATYGTTENEKTRTGEDRAVYVPGFDAVFVPGQPVHLALIAAQLAFYDMNVPILGGNS
                     WHNPELFRWAKHDLDGHIFVDGFFPNSPDPNIQMFVQRYRSQFQKEPSLFAFQAYDAA
                     TMIMETIRHGAQSGQGIWDQLVRRSDLPALSGFASFSSAGILNRRLYLLQVNNKAFTQ
                     LN"
     gene            6289..6972
                     /locus_tag="PKM72_10505"
     CDS             6289..6972
                     /locus_tag="PKM72_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNP61263.1"
                     /translation="MLVPLMAAVVLHECAHGWVANFFGDQTAKNLGRLTVNPLPHIDL
                     YGSIIVPLMLSLIPGGFVFGWAKPVPVNPAQLHNPRRDMAFVAAAGPLMNLFLAIVSS
                     LLLSLFLYLDPTIQANWPPQPGVEPRRDLLGMILVPLTAMALSSMIINIVLFSFNLLP
                     VPPLDGSRVLRSLLPVKSAEVLNHLEPYGMFLILGLLMLNSYIPIISSFIGMVFQIFQ
                     RLFLPTFIT"
     gene            6993..>7566
                     /gene="trpS"
                     /locus_tag="PKM72_10510"
     CDS             6993..>7566
                     /gene="trpS"
                     /locus_tag="PKM72_10510"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNP61264.1"
                     /translation="MTTRVLSGMQPSGLMHLGNLLGALENWKSLQTQYDCYFFVADWH
                     AMSTNYADTSRIKEYTQELLIDWLAAGIDPDRATVFVQSGIPEHAILHLLFSMIVPVP
                     WLERNPTYKEKQDEIKEKDLSTYGFLGYPVLQAADILLYKPDIVPVGKDQLPHLELTR
                     EIGRRFNNLYSPVFPEPKEHLTQFPKVMGTD"
BASE COUNT         1933 a         2066 c         1687 g         1880 t
ORIGIN      
        1 caaatggacc cgaaggacga aaccatgtgg ttcggcctgg ggaaagccta catgagcgac
       61 gaaaattgga aagaggccat tcccgccctg gagcaatgta tcctggtgaa acccgagtat
      121 tcagctgcct attttgcgtt ggctcaatca cttcaccaga cacgcgaatt tgagaagtgc
      181 cagaccatat gcaccgaagg catcaccgtg gcaaccaaga atggcgatct cctcgtcatt
      241 aaaaacctcg aagcactcaa agcgtcactc ccttcatcct aaccgctccc acctgtccca
      301 atccttttac ccaactcggg ttcacatttc ttcctgaata ctcttcagtt ctcgagcact
      361 aaggcgatat cgagcaaccg ggttcacggc caacaatcga attcgaggat cgcgtggatt
      421 ccctggtggt cgggtgaccc acgtgacctg gcgaatacga atgggattgc gtaattcctg
      481 cgccggtgct ttccataaag tcaggaggat agcactgaac tgagggacat ccacgaccac
      541 tccaactaac atcgcggcta atcgctgaag atccaaggga gggatgtcct ggggacgccc
      601 tttcccccat ggtaaatcgg gcacactgac tgctagcaac accggggcac aataccgttc
      661 taattgtctg gctttttcag ccatacgcga caacaaccgt ttgactaacg cctgttcaaa
      721 aaactcatca gaagattcta aaatctgatg aggctgtaga cgcacggcag gtgatttccg
      781 agtggcccct tgggtcgatt gaatgaccgt gacttccaca aaaaaacgat gtgtgccttc
      841 ataacactcc aagtccgccg ttcgcccacc agacgcctct aaaaatgcca ctgaaaaacc
      901 cgcttgtgcg agaaaatatg ctgtctcaat ctcggcatag gcggacgccg cctgcctgac
      961 cgggaatcga ctgatgtggt cccacagcat cgccaggcga gcctgacttt ctccatctgg
     1021 acaggcttga gcaagttcct gccattggcg aataatcaaa tccttctgag ggtgaccaag
     1081 gttcacatcc atcctattat ttcattaacg gtggacaggc ttctgcaatc tcccacatta
     1141 cgactcccat gtgtctttca ctcaaagcag gttcccggca cagaattgaa accggaatac
     1201 aagatgattc acgccacatc tttattcgaa tttaggctat cttctgggca aggaaagtgg
     1261 tgttctcaga ccggagattg ggcgtgcatt aagcgttgag gttctatcag ggggtgcgct
     1321 tcaactcttg taaggtaaaa tgcttgggga gcaaaaccgg taagacaaga ccaatgggat
     1381 ccccacgcaa tatcgtggta ggctcctcgg tagatgaatc caattggatc agaaaattcg
     1441 tgacaaaggc tgcgtagcca ggaagaaaat catccggtgt cactttatat tcctgcgttt
     1501 taatcccttc ccagatggaa aaggtccgct ttgggaaata ataaaaatga ttaggaagat
     1561 ccttcatcat catgccaagt tccatggggt aataaaaacg aatcccgcaa tagagcttgg
     1621 ggaatccact attcaaaatc gtatcaacat acattcctga aaacgatttg tagcccaaaa
     1681 gctgctcttc cggtcgaata ggcggagact gccattcaaa tccactccct ttttttcgaa
     1741 tacgacaatc cgccggcgaa cgaatcaacc acccatattg ccctgcgaag cgaaccggcc
     1801 cacaatcatc cgggatgacc ttatatccgc tggccgcaac ctcccgctgc tcagacaagg
     1861 gcatgttggt catcaacgga cgatggggat gaaaatcaag cttaagtcgt tcaggggcaa
     1921 aggcggtgga atactcccgc tcccaataaa tgacccattt ttcatctgtg cttcccggca
     1981 acgtactcat tgaatgtctt tcatggttga cctggaagca actgacctcg cttgatcatc
     2041 cccgccactt cagccacacg cttccccaag gcccaggcat cggcccgctc cacatcatta
     2101 atcccagggc tgtcgccttc cgtcgtcgct gaagcgccaa acgctcctcc gtcgctcacc
     2161 acaatcattt tgttcccgag catggctgcc aggatggtca acatcgtcac ctctttcccg
     2221 ctggatacct gtccacccgt ggcaaacgcc gctcccactt tatcgcgcat tttccattca
     2281 ggaaacacgc caaattcaaa ctgccaccgg tcaaaaaaag atttgacttc acccgacatg
     2341 ttggaccaat agacaggcga acctactatg accgcatcgc tgccaaacaa ttcatcggct
     2401 gttacctgtc ctacggtttt caggagtacg gtggttccct taaccgattc agcacctttt
     2461 ttcacggcct tggccatctc ggctgtatga ccggaaaggg aatgataggt cactaaaacc
     2521 tgaagcccat ggctagcttg cggaggtgta tggggagatg aaggttgagc gaaaacttgc
     2581 acacacccac accaccaaac aacggcaaac acagacaccc aaaggatatt tccccgaaac
     2641 accttcatga ggagcattga atggcaagaa aggcaccgat tatattcacc taaagaacct
     2701 atctaccagg aattcaatct tgtctatcgt cgttcaattt cctcgtctat ttgctcttcc
     2761 accgatacat gggttaatgc ccctcgataa tcacacttat gacaccgcac ccgaaactcc
     2821 tccaaccact tctcttgcac aaaatgttga tgacatttgg ggcaggcaaa tacccctttc
     2881 acataatgta cctgcttccg atacttcata accaacccct ccaaatttta taaattaaac
     2941 tcgatgctag cctgccacag gcctttttcc aattgcaaat ttggatacct acgatccatc
     3001 tatatgccaa cctatggaat acaaactcaa aggttccctc tattcttttt ggatagagga
     3061 aagcttacgt gccttggcat aataacgaaa aaactgcatg gaggttaacc ctgctcaaga
     3121 ctcagagccg ttgggagcat caatgaccct ggtacgttgt tttcggggaa aatattggtc
     3181 gtgaaccttt ttgaggcggg cttgctcaac atgcgtataa atttgtgtcg tggcaatgct
     3241 ggcatggccg agcatcattt ggaccgaacg aagatcggcc ccatgatcca ataaatgcgt
     3301 ggcaaacgag tggcggagca tatgaggcga aatgggcttg gtgatgcctg cgcgagccgt
     3361 gcgatccttg agaattttcc aaaaacattg ccggctcatg gcccgaccca gcctggtgag
     3421 aaataacgcc gaagatgttc gctttttgac aaaggcaggg cgggaagact gcaaataggc
     3481 cgagaccaat tggcgtgcct tatctccgat cggaacgatt cgctgtttgt cccgcttgcc
     3541 ggtcgcctgc aaaaatccga catccagatt gagatgcgcc atttccagat tgatgagttc
     3601 agacacccga agacccgtgg catacaataa ttccaccatg gcggcatcac gagaatcctc
     3661 cctggtagcc atgataggca aattgagcaa tctggtcacc tcagcctcgg acaacaattt
     3721 cggcaattga ctccattgct ttggggtccc tttcaacata ccgtcaaaag tcggatcatc
     3781 ctttccccgc tcaaatgatt tataaaaacc ccggacagaa gccaggcacc tggcaattga
     3841 ggaagaggag aggccttcgc ctttcaaact gcttaagaat tgaaaccaca acggcttgga
     3901 aagatgtgta ggatcggcaa tatcgtgtgt tcgaaaaaaa acctcaagct ttctgagatc
     3961 ccggcgatag ctctcaaccg tattcacggc atagccctgc tctacttgca acatggtgag
     4021 atagcgatcg acgaattccc tcatcagatc ttgatggcac cctcatctat gattatggaa
     4081 gaaccccaaa ctttttgatg atctcaccgg tcgtggtccc ggtcgggaga ttttctcatg
     4141 ttcttctatt ttggtaagag aatttcctga tatactaaac aagaaaagtg ttgtcgtcac
     4201 cgtattctgc tcttgaaata tccacacaca aatttattcg ggtgtaggcc tgtttccata
     4261 cggacattcc cgtccattgc caagacgtgt aggtaaattc cctttgacga ctgtcttatt
     4321 caaatatctg ccacgaatcc tattgtctct cgtactgggg agcgtgggat ccttgttaga
     4381 ccagggatgg tggcccttca tgggcggagg caccaacctg acatttgccc aacaagcatc
     4441 catttcccct cttctggatc aggcaaaatt ggagctggaa gcagataatc ctgcagccgc
     4501 caccacgact ctcgaacagt tgattgattc cttccctcct ccagaaatcc tggaagaggc
     4561 ctatctttta caagcaaccg cactgaaaca cgaccaccag ccgactgatg cccttacagt
     4621 tttaaagcaa ctcctggaag aatttccctt ttcgcgttcc acgaaccccg ctcgtgtttt
     4681 gatggccgag ctctatatcg agttagagga ctatcggcct gccctggatc aattgtatca
     4741 ggcactggac tatgccactg agctcgacct acgccggtca atatttcaat tgatccgtca
     4801 aaccgagtta gccaatcaca atccgctggg cgctgtaaaa gctttattaa atgaaatgag
     4861 tttggccgat caaacagaac ggctcgaatt agaaggcctc gctcaatccc tgattttgca
     4921 acagttagat gaacccgccc tccaggaact ggtagaaagt tatgcctccc gctatccagg
     4981 tgatatcgcg accatcagat tgattgagct gcataccgcc cacggagatg aggtcctggc
     5041 cgaacgggat atccggggat ttctcaaacg ctttcccacc cacccgtatg ctcaaactgc
     5101 gatggccctc ctgcaatcat ttatcagcaa aatcaaattc cactctcata ttgtagccgc
     5161 cgccttgcct ttttccggtc caatgaaaaa ctatggcaca gattcgttaa acggcattcg
     5221 tctcgccctc gaaatcgcca aggaacaatg gggcctgacc gcggttgggc tggtcgtcaa
     5281 agacaccacg accctcaccg ccccgttacg cgtcgagata caacaaatgc ttgatgaatt
     5341 tcagccggtt gccgttgtgg gccccttgtt atcccgtgag atacaggaac tcagccgact
     5401 ccctgatgaa aatgccatcc catttgtcac cccttcttcc acactcttga acgtgcgtca
     5461 gtttggatca ttttggttta gtaccgccat gacctcatcc gtccaagcca aacgcctggt
     5521 agaatatgcc attctcaagc ttggatatca tcgctttggc attatttatc ccaaaacagc
     5581 gtatggacgt gaaatggctg atatgttcgc taaggaagtg ttgcactccg gcggggaaat
     5641 tattgcctct gaagggtatg ccgacgacca gaccgatatt ggtgaacaat tacgccactt
     5701 gaaggaaaaa gatctggcca catacggcac gaccgaaaat gaaaaaaccc gaaccgggga
     5761 ggatcgggca gtctatgtcc caggtttcga cgccgtattt gtcccaggcc agcccgtgca
     5821 cttagccctt attgccgcac aactggcttt ttatgacatg aatgtgccga ttttaggggg
     5881 aaatagctgg cataatcctg agttgtttcg gtgggccaaa catgacctgg acggccatat
     5941 ttttgtcgat gggttttttc caaacagccc cgatcccaac attcagatgt ttgttcagcg
     6001 gtatcgcagt cagttccaaa aagaaccatc cctctttgcc tttcaagcct atgatgcggc
     6061 aaccatgatt atggaaacga tccgccatgg cgctcaatcc ggacagggga tctgggacca
     6121 acttgtgcga cgctctgact tgccggcgtt gagcggattt gcctctttta gttcggctgg
     6181 tattctgaac cggaggctct atctactcca ggtcaacaat aaggcgttta cccaactcaa
     6241 ctaagcgaca tggattccct ttcctctttt ctctataacc tctctttcat gctggtacca
     6301 ctcatggccg cggtggtctt acacgaatgc gcccatgggt gggtcgcaaa tttctttgga
     6361 gaccagactg ccaaaaacct gggacgactc accgtcaacc ccctaccgca catcgacctc
     6421 tatggaagca tcattgtgcc actgatgtta tccctgattc cgggagggtt cgtctttggt
     6481 tgggccaaac ccgttccggt caatcccgca caactccata accccagacg ggacatggcg
     6541 tttgtggccg ctgccggacc actcatgaat ttattcttgg ccattgtcag cagcctgctg
     6601 ctctccctgt tcctctatct ggaccctacg atccaggcca attggcctcc ccagccagga
     6661 gttgaaccac gaagggatct tctcggaatg atcctggtgc ccctcacggc catggccttg
     6721 tcttccatga ttattaatat cgtgttattt tccttcaatc ttctgccggt tccccctttg
     6781 gatgggagcc gggttctccg aagtcttctc ccggtcaaat ctgcggaagt cttaaaccac
     6841 ttggaaccct atgggatgtt tcttattttg gggctcctga tgctgaattc gtatatcccc
     6901 ataataagca gctttatcgg aatggtgttt cagatatttc aacgcctctt tttacctaca
     6961 tttatcactt aactagaacc ggaatactga ctatgaccac acgagtgctc agcggcatgc
     7021 aaccaagcgg gctcatgcat ttaggcaatc ttcttggggc gctggaaaat tggaaatccc
     7081 tacaaaccca atacgattgt tatttttttg tggccgattg gcatgctatg tcgacgaatt
     7141 atgccgacac cagccggatt aaagaataca cacaggaatt actgattgat tggctggcag
     7201 ccggtattga tccggatcgg gcgacggtgt ttgtccaatc cggcattccc gaacatgcga
     7261 ttctccactt gcttttctct atgattgtgc cagttccctg gttggagcga aatcctacgt
     7321 ataaggaaaa acaggatgaa attaaagaaa aagatctctc cacctatgga tttttaggct
     7381 atcccgtgct acaggctgca gacattttgt tatacaaacc cgatattgtc ccggtcggga
     7441 aggatcagct tccccatcta gaactcacgc gggaaatagg acgccgattc aacaatctct
     7501 attcacccgt gtttcccgaa cccaaggaac atctcacaca gtttcccaaa gtcatgggca
     7561 ccgacg
//
LOCUS       DAORAQ010000127         7533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1523_length_7533_cov_61.830837, whole genome shotgun sequence.
ACCESSION   DAORAQ010000127 DAORAQ010000000
VERSION     DAORAQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_7533_cov_61.830837"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PKM72_10515"
     CDS             complement(<1..934)
                     /locus_tag="PKM72_10515"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNP61265.1"
                     /translation="MWVVTAEQMQTLDRRTIQEAKVPGITLMERAGAGAMTQLTEALG
                     SPKGKKAVILCGKGNNGGDGLVVARLLAKKGAKVKVVLMAPLRELSPDAKVMYRRLSK
                     IIRPSLFTVNPSEESLHSLTQEADILVDALLGTGLSSLVRPPYSFAIEAINASQGFTV
                     AIDIPSGLDSNTGSILGVAAQADLTVTFGCPKLGLYLSSAIDKVGTIQVIDIGIPQDF
                     VSDLKPKIHLLSQGMVRPLIPLRPPSSHKGTFGHAGIVGGSQGKTGAPAMAGLGALRM
                     GTGLVTVATPESVSPILESKLLEVMTEPMPESSQH"
     gene            complement(1110..1823)
                     /locus_tag="PKM72_10520"
     CDS             complement(1110..1823)
                     /locus_tag="PKM72_10520"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNP61266.1"
                     /translation="MARLGVNIDHVATLRQARGGHEPDPIIAASLVHIAGAEGIVVHL
                     REDRRHIQDRDLTILRETVQTHLNLEMAADETVAKIALNIKPDLVTLVPERRQELTTE
                     GGLDVIQHRDRIQAMVQLLHEGGIPVSLFIDPDINQLRAAHKIQADCVELHTGRYANT
                     RRQQEEDAELDALVQTAKLASKLGLRVSAGHGLNYRNIKRLTSIPEIEEFNIGHSIIA
                     HAIFVGLERAVRDMKHLLG"
     gene            2074..3261
                     /locus_tag="PKM72_10525"
     CDS             2074..3261
                     /locus_tag="PKM72_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNP61267.1"
                     /translation="MEPFYRIQRLPGYVFSQVQELKLQARKRGEDIIDLGMGNPDQPT
                     PRHIVDKLIETARLGRNHRYSASRGITKLRHAICDWYQRNYEVSLDPETEAIVTLGVK
                     EGLAHLALAMVGPGDVVLAPTPTYPIHMYSVIIAGGEIRGVELGPDSDFFENLKKAFK
                     QTQPAPRALIISFPHNPTTRVVDLEFFKKVVEFALEHQIWVIHDLAYADIVFDGYKAP
                     SLLQIPEAKQIGVEFYSLSKSYNMPGWRVGFCVGNREVIGALTKIKSYLDYGMFQPIQ
                     IASIIALNGPQDCVKEIVGRYERRRNALVRGLNRIGWQIDYPRATMFAWAKIPQPYLG
                     MGSLEFSKLLLRDAKVAVSPGIGFGEGGDDYVRFALVENEHRIFQAVRGIRQALKFDG
                     DSE"
     gene            3258..4568
                     /locus_tag="PKM72_10530"
     CDS             3258..4568
                     /locus_tag="PKM72_10530"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNP61268.1"
                     /translation="MKKAIQVGLIGFGTVGTGVVKILQENAGLIAKRVGVPVSLVRIA
                     DLDVTTDRGVSVAQGVLTTDVRGILDDPAIDIVLELIGGHEPAKRFILQALERKKSVV
                     TANKALLADHGEEIFEAAFKAGVDLGFEASVGGGIPIIRSLTEGLAANRILSITGIMN
                     GTSNYILTRMTHERRDFDEILQEAKREGYAEADPSLDVDGVDAAHKLAIMVNLAYGTP
                     VPMRSIFTEGISRISTVDIEFARELGFTIKLLGIAKLQEHAIEARVHPALVPAGSPIA
                     QVNGVYNAIHLIGDAVGDVVLYGKGAGSLPTASAVVGDIIDLARNRLAGVAGRVPVTS
                     FQWESRVPIPIKPMDDIESRYYLRCMVKDQPGVLSAISGILGQRGISISSVLQKGRKE
                     GQTVPLVILTHRSIERAVQSALQEINALSMVSEATTLLRMEGVE"
     gene            4610..5671
                     /gene="thrC"
                     /locus_tag="PKM72_10535"
     CDS             4610..5671
                     /gene="thrC"
                     /locus_tag="PKM72_10535"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNP61269.1"
                     /translation="MIPWRGIIEEYRKFLPVGDQTPIVSLQEGNTPLIPAKRLAAKVC
                     PGMDLYLKIEGANPTGSFKDRGMTMAVSKAVENKARGLMCASTGNTSASAAAYGAKAG
                     LPVYVVIPAGNIALGKLTQALMHGAQVIQIDGNFDQALTIVKDLCQETAIELVNSINP
                     FRLEGQKTGAMEICDQLGYAPVIHALPVGNAGNITAYWRGYREYQEAGQTQGLPRMFG
                     FQAEGAAPMVRGHVVESPKTIASAIRIGNPASWNFALKALEESQGHLDMVTDEEIIRA
                     YQLVASEEGVFCEPASAASLAGIDKMNRAGLLPKEGVIVCTLTGHGLKDPDTAISVSY
                     RPVTVPATHEAVRAQLTNT"
     gene            5745..6980
                     /locus_tag="PKM72_10540"
     CDS             5745..6980
                     /locus_tag="PKM72_10540"
                     /inference="COORDINATES: protein motif:HMM:NF021646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61270.1"
                     /translation="MRKTIVIHVDGLTGQSYQELGDQTVLQSARTPHLDELAGHGELG
                     RLGVPRETRPFCGEMALLAVLGYDPHKWYTGPGAFEAGGLEVVLDAHDVAYLCHLVTL
                     RGQEGWGDGKKLGPQLFMADPFGGEIGTEDARELIDAVNEQLISESIQFYMGSHHRHL
                     MVWVGASGKVACRSPYEALGESIEAYLPAGDGSQILRELMAASRAILCHHPVNQERLN
                     AGLKPANCLWLWGSGKPVELPPLKERWPVHGMVISPDGPYVGVGMAAGLETVRVEHGG
                     GGDLAWLQALANRTLTALENHDLVCLHIPFYEWAFKDGQAVSPSQLGEYLGQVDEQVI
                     GAIHQSDGSKDSHRIVVLVTPSPHGHNDGGAPVSPYLVFEGSEGKSEIHAVPLNEREV
                     ANRPLRDATKLFERFLGTH"
     gene            6986..>7533
                     /locus_tag="PKM72_10545"
     CDS             6986..>7533
                     /locus_tag="PKM72_10545"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNP61271.1"
                     /translation="MARYIQKFGGTSVGSLERIQNVARLIERTYREGHQLVVVLSAMS
                     GETDRLMKLAKTLSIDPDDRELDVLLSSGERVTIALMAMTLKSLGMKAQSFTGRQVGI
                     VTDSMHTKARVSRVDTDRVLGALRDGIIPIVAGFQGINAASDVTTLGRGGSDLTAVVL
                     AAALKADTCFIYTDVDGVYTADP"
BASE COUNT         1790 a         1765 c         2170 g         1808 t
ORIGIN      
        1 ggtgttggga cgattccggc atgggctctg tcatcacttc cagaagcttt gactccaaga
       61 tgggcgaaac gctttcaggg gtggctacgg tcaccagtcc ggttcccata cgaagtgccc
      121 ctaggccagc catagcggga gcccccgtct ttccctggga gccaccaaca atccctgcat
      181 gtccaaaggt ccctttatgt gaggacggag ggcgaagagg aatgagggga cgaaccatcc
      241 cctgagacag aagatgaatc ttgggcttca gatccgacac aaagtcctgg ggaataccaa
      301 tatcgatgac ctggatggtt ccaactttgt cgatggctga actcaaatac aggccgagtt
      361 tcgggcatcc gaaggtcacg gtgagatcgg cctgagcagc gactcccagt atggacccgg
      421 tattgctgtc caatcctgac ggaatatcga tggccacggt aaagccttgc gaggcattta
      481 ttgcttcaat ggcaaaggaa tagggggggc gaaccaaaga agagaggccc gtaccaagga
      541 gcgcatcgac aagaatgtcg gcttcttgag tgagggaatg cagggactcc tccgagggat
      601 taacggtaaa cagagatggt ctgatgatct tgcttagccg ccgatacata accttcgcat
      661 cgggactgag ttcccggagc ggagccatca agacgacctt cacctttgcc ccttttttgg
      721 ccaggagcct ggccacaaca aggccatctc caccattatt gcccttgcca caaagaatga
      781 cagccttttt gcctttaggg gaacccaagg cttctgtaag ctgggtcatc gcaccggctc
      841 ccgcccgctc cattaaggtt atgccgggaa ccttggcttc ttggatcgtt cgccgatcaa
      901 gcgtttgcat ctgttcagcg gtcacaaccc acataggcag atcccaggag caaggagaaa
      961 tgaatagtta aagcatgagt aagaataagt caggcgtaga cgggtcagga tgatatttga
     1021 ggtctgcacg gtttgtgaat tgactggtgc gtgtctccgg gtcattcata aaatcaagtt
     1081 ggggagatcc tgctttttaa tcgtagacct taacccagga ggtgtttcat gtcgcggact
     1141 gctcgctcaa gcccaacaaa tatggcatga gcaataatgc tatgaccaat gttgaactct
     1201 tcaatttcgg gaatgctggt cagccgcttg atattccggt aattcagtcc atgccctgca
     1261 cttacacgca atcctagttt cgacgcaagt ttggcggttt gcaccaatgc gtcaagctca
     1321 gcatcttcct cctgctgacg gcgggtattg gcgtaccggc cggtatggag ttccacgcaa
     1381 tctgcctgga ttttgtgggc ggcacgaagc tgattgatat caggatcaat gaacagactg
     1441 acgggaatgc cgccttcatg gagtaattgc accatggctt gaatacggtc acgatgctgg
     1501 atcacgtcca acccaccctc tgtggtaagc tcctggcgcc tttcaggcac gagtgtcacc
     1561 agatcgggtt taatgttcag ggcaattttg gccacggtct cgtctgcggc catttctaag
     1621 ttgagatgcg tctgcacggt ttcacgcaaa atagtcagat cccgatcctg aatatgccgc
     1681 cgatcctccc ggagatgcac cacaatgccc tcggctcctg caatgtggac caaagaagcg
     1741 gcaataatgg ggtccggttc atgtccgcca cgagcttggc gaagggtcgc cacatggtcg
     1801 atattcacgc cgagtcgtgc cataaaagtc ctttaaagtc tgggtgaata aagagccaaa
     1861 gagatctcat tatggcaaaa gggaaaatct tcccgcaata aatggaaatt ttgtggcgtg
     1921 agagggaatt ggataaaata actgaaaatt cccattctct gaaattgact ggtcgtgtcc
     1981 cctcatctag aataaaaact ctattaaatc gtacgattat ctatccattc ccttccaaaa
     2041 ccatcaagga cgattcgtca ttttacacaa acaatggaac ctttttaccg aattcaacga
     2101 ctcccaggat acgtcttctc tcaggttcaa gagttaaaac tccaagcccg gaagcgggga
     2161 gaagacatca ttgatttagg aatgggcaat cccgaccagc ccacaccccg tcatatcgtg
     2221 gacaaattga ttgagaccgc gcgactcggg cggaatcatc gctattcggc ctcgcggggt
     2281 attacaaaat tgcgccatgc catctgcgat tggtatcaac gaaattacga ggtcagtctt
     2341 gatcccgaga cggaagcgat tgtgactctg ggggtgaaag aaggcctggc gcatttggcc
     2401 ctggccatgg tgggaccggg tgacgtagtc ctggcaccaa cccccacgta cccgattcat
     2461 atgtacagtg tgatcatcgc gggtggggaa atccgtgggg ttgaactcgg gccggattca
     2521 gattttttcg aaaacctgaa aaaagccttt aaacagacgc aaccggctcc ccgggcgttg
     2581 atcatcagtt tccctcataa tccaaccact cgcgttgttg acctggagtt ttttaaaaag
     2641 gtagtggagt ttgccctgga acaccaaatc tgggtcattc atgatttggc ctatgccgat
     2701 atcgtgttcg atggttacaa agcccccagc cttcttcaaa ttccggaggc caaacaaatt
     2761 ggcgtggaat tttactcact ctcgaagagt tataatatgc cgggttggcg ggtaggattt
     2821 tgtgtcggga atcgtgaagt gattggtgcg ttgaccaaaa tcaaaagtta cctggattat
     2881 ggcatgttcc agcccattca aattgccagt attattgcct tgaacggtcc ccaggattgc
     2941 gtgaaagaaa ttgtgggacg atatgagcga cggcgaaatg ctctggtccg tggacttaac
     3001 cgcattggtt ggcaaatcga ctatcctcgc gccaccatgt ttgcctgggc aaaaattcca
     3061 caaccctacc tcggaatggg atcgttggag ttttccaagc tcttgttacg agatgccaaa
     3121 gttgcggttt ccccgggaat aggattcggc gaaggcggcg atgactatgt ccgctttgca
     3181 ttagtcgaaa atgagcaccg gattttccag gcggttcgag gaatccgtca ggctttaaaa
     3241 tttgacggag attcagaatg aaaaaagcca tccaggttgg attaatcggg tttggtaccg
     3301 taggaaccgg ggtcgtgaag attcttcagg agaatgccgg tctgattgca aaacgtgtcg
     3361 gagttccggt ttccctggtg cggattgcgg acttggatgt gacaaccgat cggggagtct
     3421 ctgtggctca aggggtattg acgacggatg ttcggggcat tctggatgat cctgcgattg
     3481 atattgtcct ggaattaatt ggcggacatg aaccggccaa acggtttatt cttcaggcgc
     3541 ttgaacggaa aaagtctgtg gtgacggcga ataaagcgtt gttagccgac catggtgaag
     3601 aaatctttga ggcggcattt aaggcaggcg ttgatctcgg gtttgaagcg agtgtcggcg
     3661 gaggtatccc catcattcga tcgctcaccg aagggttggc tgcaaaccgg atcttgtcga
     3721 ttacggggat catgaatggt acgtcaaatt atattttgac gaggatgacc catgagcgcc
     3781 gtgatttcga cgaaatttta caggaagcga aacgggaagg gtatgccgaa gcggatccat
     3841 ccctggatgt tgatggggtg gatgccgctc acaaactggc tatcatggtg aaccttgcgt
     3901 atggaactcc cgtgcccatg cggtccatat tcaccgaggg catttcccgt atttcgacgg
     3961 tggatatcga gtttgcccgt gaactcgggt ttaccattaa attgctggga atcgccaaac
     4021 tgcaagagca tgccattgag gcacgagtcc atcccgccct ggtgccggcc ggatctccca
     4081 tagcacaggt caatggcgtc tataatgcca ttcatttgat tggtgacgcc gtgggagacg
     4141 ttgtgctcta cggcaaaggg gccggatccc ttccaacagc cagcgccgtg gtaggggaca
     4201 ttatcgatct tgcacgcaat cgcttagccg gggttgccgg acgggttcct gtgacctcat
     4261 tccaatggga atcgcgtgtg cccatcccta ttaagccgat ggacgacatt gaaagccggt
     4321 attatttgcg gtgcatggtc aaggatcaac ccggggtcct gtcggccata tcagggattc
     4381 tcgggcagcg tgggatcagc atttcgtccg tcctccaaaa agggcgaaag gaaggacaaa
     4441 cggtgccgtt ggtcatcttg acgcatcgtt ccatagagcg tgccgttcag tcggcattac
     4501 aggaaattaa tgcactttcc atggtttctg aggcgacgac gttactgcgt atggaggggg
     4561 tggaataaga gatgggggca cgttgtttcg taaaaaggga caagccatta tgatcccctg
     4621 gcgtggcatt atagaggaat accggaaatt tctcccggta ggagatcaga cgccgattgt
     4681 gtctcttcag gaggggaata cgccactgat cccagcaaaa cgcttagccg cgaaagtgtg
     4741 tccgggaatg gatttgtatc tgaagattga aggggcgaat ccaaccgggt cctttaagga
     4801 tcgggggatg accatggcgg tttctaaagc cgtggaaaac aaagccaggg gactcatgtg
     4861 cgcttcgacg ggcaatactt ccgcctcggc agcggcctat ggggcgaaag cgggtttgcc
     4921 ggtgtatgtc gtcattccgg cagggaatat tgcattgggt aagctgaccc aggctctcat
     4981 gcatggtgcc caggtcattc agattgatgg gaacttcgat caggctttaa cgattgtgaa
     5041 ggatttgtgt caggaaacgg cgattgagtt agttaattca attaacccgt ttcgtttgga
     5101 aggccaaaaa acgggggcca tggaaatctg tgaccagttg ggatatgccc cggtcattca
     5161 tgccctccct gtgggaaatg cagggaatat tactgcgtac tggcgagggt atcgggagta
     5221 ccaggaagcc ggacaaacac aagggttgcc tcgtatgttc gggtttcagg cggaaggagc
     5281 ggctcccatg gtcagaggcc atgtggtgga gtcaccgaaa actattgcct cagccattcg
     5341 gattggaaat cctgccagtt ggaattttgc cctgaaagcg ttagaggaat cacagggaca
     5401 tctggatatg gtgacggacg aagaaattat tcgggcttat caattggtgg cgagtgaaga
     5461 aggggtcttt tgcgagcccg cttcggctgc ttctcttgca ggaattgaca aaatgaatcg
     5521 agccgggttg ttgccaaaag agggagtcat tgtgtgtacc ttaaccggac atgggttaaa
     5581 ggacccggat acggccatct cagtctcgta tcgaccggtc accgttcctg ccactcatga
     5641 ggcggtcaga gcgcaattga caaatactta atccttcatg acctcctgaa aaccaagatc
     5701 gttcatacaa ctactaactc aatcaacata gtgaggccaa gatcgtgcga aaaacgatcg
     5761 tgattcacgt cgatggtttg acgggacagt cgtatcagga attaggagac caaaccgtct
     5821 tgcaatcagc tcggacaccg catctggatg aattggctgg gcatggtgaa ctgggacgtt
     5881 taggggttcc ccgggaaacc cgtccatttt gtggggaaat ggcactcctt gccgtcttag
     5941 gatacgatcc acacaaatgg tatacgggtc ctggtgcttt tgaggctgga ggattagagg
     6001 ttgtgctgga tgcccatgat gtggcctatc tgtgtcattt ggtgactctc cgtggtcagg
     6061 agggatgggg ggatgggaaa aaacttggtc cgcagctatt catggcggac ccctttggcg
     6121 gagaaatcgg gactgaggat gctcgtgaac tcattgatgc cgtcaatgaa caattgatat
     6181 ctgaaagtat tcaattttac atgggtagcc atcatcgcca tcttatggtc tgggttggtg
     6241 cgagcggaaa agtggcctgt cgcagcccat acgaggcctt gggagagtcg atcgaggcgt
     6301 atctgcctgc tggtgacgga tctcaaattt tacgtgaact gatggcggcc tccagagcga
     6361 ttctatgcca tcatcctgtg aatcaggagc gtctgaatgc cgggttgaag ccggcgaatt
     6421 gtttatggtt gtgggggtca ggaaaacctg tggagttgcc tcctttaaaa gaacggtggc
     6481 cagtccacgg tatggttatt tccccggatg ggccgtacgt aggcgttgga atggccgctg
     6541 gtcttgagac ggtcagggtt gaacatgggg gaggaggaga cctcgcctgg ttgcaggcct
     6601 tggccaacag aactttgact gcgttggaaa atcacgacct ggtctgtttg catattccct
     6661 tttatgagtg ggctttcaag gatgggcagg cggtgtcacc gtcacaatta ggtgaatatc
     6721 ttgggcaagt ggacgagcag gtgatcggag ccatccatca gtctgatgga agcaaggact
     6781 cacatcggat cgtcgtcctg gtgaccccaa gtccgcatgg gcacaatgat ggaggggctc
     6841 ctgtctcgcc atatctggtg tttgaagggt cggaggggaa atccgaaatt catgcggtcc
     6901 cattgaatga acgggaagtg gccaaccgtc ccctccgtga tgccaccaaa ttgtttgagc
     6961 ggtttctggg gactcactaa atcctatggc acggtatatt caaaaattcg gcgggacgtc
     7021 ggtggggagt ctggaacgaa tccaaaacgt cgcccggctt attgagcgga cctaccggga
     7081 ggggcatcaa ctggtggtcg tgttgtctgc gatgagcggg gaaacggatc ggctcatgaa
     7141 gctggccaag acgttgagca tcgacccgga tgaccgggaa ctcgatgtct tgctgtcctc
     7201 cggagagcgt gtcacgatag cgctgatggc gatgaccctc aaaagtcttg ggatgaaggc
     7261 gcaatccttt acgggacgtc aggtagggat tgtcaccgat agcatgcata ccaaagcccg
     7321 ggtttcccgg gttgataccg accgggtgct gggagccttg cgtgacggga ttattcccat
     7381 tgtggccggg tttcaaggga tcaacgccgc ctccgatgtg acgacgttag gacgcggggg
     7441 atctgatttg acggccgtgg tgttggcggc ggcgctcaag gccgatacct gctttattta
     7501 taccgatgtg gatggggtgt acacggccga tcc
//
LOCUS       DAORAQ010000128         7445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1538_length_7445_cov_63.803383, whole genome shotgun sequence.
ACCESSION   DAORAQ010000128 DAORAQ010000000
VERSION     DAORAQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1538_length_7445_cov_63.803383"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..587
                     /gene="tsf"
                     /locus_tag="PKM72_10550"
     CDS             9..587
                     /gene="tsf"
                     /locus_tag="PKM72_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNP61272.1"
                     /translation="MVKELRGKTGAGILDCQKALQDTENNVEKAIDLLRQKGLAAAQK
                     KAGRETKEGIISSYIHSGSKIGVLIEVNCETDFVARNEEFQAFVKEVALQIAASHPLY
                     IKREDVPADLIEREKNIYLAQIKESGKPEASWEKIIKGKLEKYFQEQCLLEQGFIKDP
                     SISIQDLLSQKIAKLGENLTISRFTRYQLGQD"
     gene            587..1315
                     /gene="pyrH"
                     /locus_tag="PKM72_10555"
     CDS             587..1315
                     /gene="pyrH"
                     /locus_tag="PKM72_10555"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNP61273.1"
                     /translation="MSLKYRRVLLKISGEMLAGEQTYGIQPSVLHNLADEIADVLTMK
                     VEIAIVIGGGNIFRGLAASASGMERASADYMGMLATMLNALALQNALEKKDVPTRVLS
                     AIEMRQLAESYIRRRAIRHLEKKRVVIFAAGTGNPYFTTDTAAALRAMEIGADVIMKG
                     TKVDGIYDADPMTTPTAKRFTELPFLSVLNKSLKVMDATAITLCMDNDLPIIVFNLTS
                     SGNIRKILEGEPIGTIVSKNALVT"
     gene            1330..1887
                     /gene="frr"
                     /locus_tag="PKM72_10560"
     CDS             1330..1887
                     /gene="frr"
                     /locus_tag="PKM72_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNP61274.1"
                     /translation="MNDPTILKTTQRMEGAIEHLKREFMSLRTGRASLGLLDHITVDY
                     YGTPTPLKQVANLAIPESRMITIQPWDTSQIREIERAIISSELGLTPSNDGKLIRLPI
                     PPLSEERRKDLVKLSKKYGEETKVQIRGFRREGNDELKHLHKESSLTEDAKGRLETEI
                     QKLTDKFIQAVDDLTKKKEEEILAI"
     gene            1897..3120
                     /gene="alr"
                     /locus_tag="PKM72_10565"
     CDS             1897..3120
                     /gene="alr"
                     /locus_tag="PKM72_10565"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNP61275.1"
                     /translation="MIPIRPPSPSDLRHLPPPITVSIRLDALTRNFQQIRQSITSSTK
                     VLAVIKADAYGHGAIPVAQTLEHLGIFGFGVASVMEGIVLREHHIRCPILVMGPLLPE
                     HLPEIIRHQLTPVISRAEIVQQLLELLSPRATPFPIHLKVDTGLHRLGFETDQALSLL
                     TGLSLSTPLIEIQGLLSHFADADNQDHTLTNRQIQSFLAFIDQAKQLGIQKPILHMAN
                     SAGILFHPTAHLGMVRPGLMLYGYAPRQESSPQSLTLEPVMQATTYVAHLRSLQPGEI
                     VGYNALFRTRRPSQIAVLPVGYTHGFPRHLTGIGHVLLKGEAAPIIGKICMDMMMVDV
                     TDIPHPTVGDEVMLLGKQGTREITAEDHAGWLKTIPYEVLCGIGGKANRIYLPHIPDS
                     SPSPAQKPSAKKILE"
     gene            complement(3185..3493)
                     /locus_tag="PKM72_10570"
     CDS             complement(3185..3493)
                     /locus_tag="PKM72_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNP61276.1"
                     /translation="MVLLEFSMSPLGKGESVGKYVSRSLDLIDKSGVDYRLNPMGTVL
                     EGEWDDVFRVVKECYTRMKKDCPRISCVIKVDYRRGKKGRLAGKVASVEKHLKRKLKT
                     "
     gene            complement(3493..4149)
                     /locus_tag="PKM72_10575"
     CDS             complement(3493..4149)
                     /locus_tag="PKM72_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNP61277.1"
                     /translation="MTKNAGFQVTLDLDGRQCMVIGGDEEAVEKVHRLLDAGAKVTVV
                     SPTLHVDLRKLTASGKIIHRGRTFRSGDAQGVVLIMNVLKDDLDLAKSLFELAKTERC
                     LVWSMDLPEYSTIIMPALVKRGNLRIAISTSGTAPALAKVLRQNLELLFDDEFDQFLD
                     WLGALREELKSTEVSDRRRRERLAEAVNGFQLTGEMEYPNSWKLSSEEQVEPVGKEGG
                     "
     gene            complement(4360..5259)
                     /gene="metF"
                     /locus_tag="PKM72_10580"
     CDS             complement(4360..5259)
                     /gene="metF"
                     /locus_tag="PKM72_10580"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNP61278.1"
                     /translation="MHISEVLRTHHPAISFEFFPPKTERTFQELFEAISALMPLKPAY
                     VSVTYGAGGSTRERTHDLVVKLKRETDLTIVSHLTCVGSGRQEIHQILSKYQASGIHN
                     IMALRGDPPKGCTAVEIPEDGFQFASDLVWFIKQQFPEMGVGVAGFPEGHPGTPNRLQ
                     EMDNLKRKVDAGADYICTQLFFDNRDFFDFCERCEVVGIKVPIIAGIMPIVSRKGMAR
                     MSELALGARIPAKLLRAMDRAENDVYAEGVGIHWATEQVVDLIDHKVKGIHFYTLNKS
                     KATLKIYESLGIQSSESLQHPVE"
     gene            complement(5302..6381)
                     /locus_tag="PKM72_10585"
     CDS             complement(5302..6381)
                     /locus_tag="PKM72_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61279.1"
                     /translation="MVDGFLLFAVLDVWRMVLPLGLTLLLLVTELLCCWQGDSYQRGL
                     RVVLNSLVVRLTIGLLVMSSVIGLLVPAILGFPLPESHIAVGPWSYEEMLGFVLGGFC
                     LWKSLQKNGPGKHSGMVWLGCASVLFFGAGVLWLARWDAMAHEGHVAGGWFVLGLSTV
                     DWTRVVPKSLHLLFAGLAAGGLVVTLLGLLGWSGGPVATDSTSPSRFLPSDGQVRYGV
                     GWMLAGLVPQMFIGPWLFLVLGEGPRGGLIDGAGLASAFFIVGVMAALLAMVLLNASF
                     MVPHVKGLVWGGIICTVITLVLMGMVRYVMFLSTLEGQGIPIAIGNVTVFHLLTVLVL
                     TGLVGAILVRWCVRPLSAFHAKVEL"
     gene            complement(6756..>7445)
                     /locus_tag="PKM72_10590"
     CDS             complement(6756..>7445)
                     /locus_tag="PKM72_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNP61280.1"
                     /translation="ILGPNGSGKSTFMKLLSRELYPVVGPDRFVRLMGKEHWNVWELR
                     SQIGIISSDMQQAYPGHTTGLEIILSGFFSSMAVYDHQTISLRHRNESAEMMRRLGID
                     HLRDRPFGEMSTGEQRRALLGRALVHEPQILVLDEPTTGLDVQACFQYLQIIRDFIHR
                     GGTVILVTHHVHEIPPEIDRVVLLKQGRIMEDGLKGDVLNDHRLSVLFETPVHLLHNR
                     GWFQIVQASPD"
BASE COUNT         2139 a         2134 c         1580 g         1592 t
ORIGIN      
        1 tgagtgcctt ggtgaaggag ttgcgaggca aaacaggggc aggaatcctt gattgtcaaa
       61 aagcccttca agacacagaa aataatgttg aaaaagccat cgatctgttg cgccaaaaag
      121 ggttggctgc tgcgcaaaaa aaggcaggac gagaaaccaa agagggcatt atctcctctt
      181 atattcattc aggatcaaaa atcggagtcc tgatcgaagt caattgtgaa acagactttg
      241 ttgcacgaaa tgaagaattt caagcgttcg tcaaagaagt ggctctacaa atcgcggcct
      301 ctcatccttt gtatattaaa cgtgaagacg ttccggctga cttgattgaa cgggaaaaaa
      361 atatctatct cgcgcagata aaggaatcgg gcaaacccga agcgagttgg gaaaaaatca
      421 tcaaagggaa attggaaaaa tactttcagg aacaatgcct cctggaacaa ggcttcatta
      481 aggatcccag catcagcatc caagatctcc tttcgcagaa aatcgcaaaa ttaggggaaa
      541 acctcacgat cagtcgcttt acgcgctacc aactcggcca ggactaatgt cgctcaaata
      601 tcgccgggtt ctcttaaaga tcagtggaga aatgcttgct ggggaacaaa cctacggcat
      661 tcagccttcc gttttacata acctcgcaga cgaaattgcc gacgtcctga ccatgaaggt
      721 ggaaatcgcc attgtaattg gaggtggcaa cattttcaga ggcctcgctg cgagtgcctc
      781 tggcatggaa cgagcttcgg ccgattatat gggcatgctc gcgaccatgt tgaatgctct
      841 ggctttgcaa aatgcgttag agaaaaaaga cgtccccacc agggtcttat cggccattga
      901 gatgcgtcag ttagccgaaa gctacattcg tcgtcgggct attcggcatt tggaaaaaaa
      961 acgtgtggtg attttcgccg ctggcaccgg caatccatac tttaccaccg acacggccgc
     1021 cgcgctccgg gccatggaaa ttggtgcgga tgtgatcatg aaaggcacga aggttgatgg
     1081 aatctacgac gcggacccta tgacgacacc cacggcaaaa cgtttcacgg aattaccatt
     1141 tttatcggtg ctcaataaaa gcctgaaagt catggatgcc acagccatca cgctgtgcat
     1201 ggataacgat ctgcctatca ttgtcttcaa tctgacgtct tcaggaaaca tcagaaaaat
     1261 ccttgaggga gaaccgatcg gcaccattgt ctccaaaaat gcgcttgtca cttaacgtgg
     1321 ggaatgatca tgaacgatcc aaccattctt aaaactactc agcgaatgga gggagccatc
     1381 gagcacctca agagagaatt catgtctctc cgaaccggtc gtgcttcatt aggactactc
     1441 gaccatatta ccgttgatta ttacgggacg cccactcccc tcaagcaggt tgccaacctc
     1501 gcgattcctg aaagccgcat gatcaccatt caaccctggg atacctccca aatccgggaa
     1561 attgaacgtg ccatcattag ttcggaattg ggactcactc cctccaatga cggaaaactc
     1621 attcggctcc ccattccccc attgagcgaa gaacgacgga aggatttagt caaactcagt
     1681 aaaaaatatg gggaagagac caaagtacag attcgtggat ttcgtcgtga aggcaatgac
     1741 gaactcaaac accttcacaa agaaagctca ctgacagaag atgccaaggg ccgcctggaa
     1801 accgagatcc aaaaactcac agacaaattc atacaggccg tcgatgacct caccaagaaa
     1861 aaggaggagg aaatactcgc gatctgagga atatttgtga taccgatcag gccaccttca
     1921 ccttccgacc ttcgacacct tccgcctccg atcaccgttt ctatccgtct tgatgcgcta
     1981 accagaaatt tccaacaaat ccggcaatcg atcacttcgt cgacaaaagt tctggccgtc
     2041 attaaagccg atgcctatgg ccatggggca atccctgtag ctcaaaccct ggaacacctg
     2101 ggcatctttg gatttggcgt cgcatcggtc atggaaggca tagtccttcg agaacaccac
     2161 atccgctgcc ccatcctggt catgggacca ctgctaccgg aacatctacc cgaaattatt
     2221 cggcatcagc taacaccggt gatttcacgg gccgaaattg tccaacaact tctggagcta
     2281 ctttctcctc gggccacccc ctttcccatt cacctcaaag tcgacaccgg attacaccga
     2341 ctcggcttcg aaactgatca agccctctct ctactcaccg gactatcact ctcaactcct
     2401 ctcattgaaa tacagggcct cctgagccac tttgctgatg ccgacaatca ggaccacaca
     2461 ttaaccaata ggcaaatcca atcatttctg gcatttatcg accaggcaaa gcaactgggg
     2521 atccaaaaac cgattcttca tatggccaat agcgcaggaa tcctctttca tccaactgcc
     2581 catttgggca tggtcagacc tggattaatg ttatatggat acgcaccgcg gcaggagagt
     2641 tcgccacagt ccctcacgct tgaaccggta atgcaagcca ccacatacgt ggcccatctc
     2701 cgctcacttc agccgggcga aattgtggga tataatgctt tgttccgcac acggagacca
     2761 tctcaaatcg ccgtcctgcc ggtgggctat acccacggat ttccccgtca tctcaccggc
     2821 atcgggcatg tcctactcaa gggagaagcg gccccaatta ttggaaaaat ttgcatggat
     2881 atgatgatgg tggatgtcac ggacatcccc catccaactg ttggagacga ggtcatgcta
     2941 ttgggaaaac agggaacgcg ggaaataaca gccgaagatc atgccggatg gttaaaaacg
     3001 attccctatg aagtcttatg cgggatcgga ggaaaggcaa accggattta ccttccccac
     3061 attcctgact cttctccgtc accagcacaa aaaccgtcag caaaaaaaat cctggaatga
     3121 ccacctagct gacggattca ttccccacac aaccacattg actcaggcaa agggttttag
     3181 catcttaggt tttcaactta cgctttaaat gtttctcgac acttgccacc ttgccagcca
     3241 accggccttt cttcccacgc cgataatcaa ccttaatcac acaagaaatc cgagggcaat
     3301 cctttttcat tctggtatag cattctttca cgacgcgaaa cacgtcatcc cactcgcctt
     3361 ccaacacggt tcccatggga ttgaggcgat aatccactcc gcttttatca atcaggtcta
     3421 gtgaccggga cacatatttt ccgacactct cgcctttgcc aagaggcgac atgctaaatt
     3481 ccaacagcac cattacccac cttcctttcc aacgggttcc acctgctcct cactggacaa
     3541 cttccaacta ttgggatact ccatttcccc ggtcaattga aagccattga ccgcttccgc
     3601 caaccgctca cgacgccgac ggtcgctcac ttccgtactc tttagctctt cacgcagggc
     3661 gcccaaccaa tctaggaact ggtcaaattc atcatcaaat agcaattcaa gattttgacg
     3721 aagcaccttc gccagagccg gagcggtccc gctcgtacta atcgctattc gaagattccc
     3781 gcgcttaacc agcgcaggca taatgatcgt cgaatattct ggcaaatcca tcgaccacac
     3841 cagacagcgt tccgttttcg ccagctcgaa caaggacttg gccaaatcca gatcgtcctt
     3901 caacacattc ataatcagca caaccccttg agcatcaccg gatcgaaagg ttcgtccccg
     3961 atgaataatt ttccctgagg cagtcaattt ccgcaaatca acatgaagag taggacttac
     4021 cacagtaact tttgctccgg catccaacaa ccgatgcacc ttttcaacgg cctcttcatc
     4081 accaccaatc accatacatt ggcgaccatc caaatcaagt gttacctgaa accctgcatt
     4141 cttcgtcatg aatatcctcc tttagaaccc gacacatctt acccaacatg caccacaggg
     4201 ggaaagggta aaatccccaa tcccatggcc caccaacttg cataggtcgc aggcgccaaa
     4261 acgcacgaaa aagaacacgg agaacaggtc gagagaactg agagacggaa tgcctattac
     4321 aggggtgttg agaaacagac aggaaaaatc aggcagggtt tattcgaccg gatgctgaag
     4381 actttctgaa ctttgaatac cgagagactc ataaattttc aaggttgcct tcgatttatt
     4441 caaggtataa aaatgaatac ccttcacctt atgatcaatc agatccacca cctgttcggt
     4501 cgcccaatga atgcccaccc cttccgcgta aacatcattc tccgctcgat ccatcgcccg
     4561 caaaagtttt gccggaattc gtgcccccag cgccagctcg gacatgcgag ccatgccctt
     4621 cctggaaaca atcggcataa tgcccgcgat gatcggtact ttaatgccca caacttcgca
     4681 acgttcacaa aaatcaaaaa agtcccgatt atcaaaaaac aattgggtac agatataatc
     4741 agcacccgca tcaacttttc gcttcagatt gtccatttcc tgtaaccggt taggcgtacc
     4801 gggatgtcct tcgggaaatc ccgccactcc cacacccatt tcaggaaact gctgcttaat
     4861 aaaccacacc agatcagacg caaactgaaa gccatcctca ggaatctcca cagccgtgca
     4921 tcccttggga ggatctcccc gcaaagccat aatattatga ataccactgg cctggtattt
     4981 tgagagaatc tgatgaattt cctgacggcc tgagcccacg caggttaaat gcgacacaat
     5041 cgtcagatcg gtttcccttt tcagtttcac gactaaatca tgcgtgcgtt ccctggtaga
     5101 cccaccagcc ccataggtaa cactgacata cgcaggcttt aacggcatga gagcggaaat
     5161 cgcttcaaac agttcttgaa acgtccgttc cgtttttggg ggaaaaaact cgaaactaat
     5221 cgcaggatga tgcgtccgca acacctcaga aatatgcatg gtcatacctt aaatcgctca
     5281 cccacatttg tctagtctct atcacaattc caccttggca tgaaaggcag aaagaggtct
     5341 cacacaccaa cgaacgagaa ttgcgcccac aagacccgtc aaaaccagaa cggtcagaag
     5401 atgaaacacg gtcacattcc caatcgcaat gggaatcccc tgcccctcta gggtgctcaa
     5461 aaacatgaca taccggacca tccccatcag taccagggtg atcaccgtac aaataattcc
     5521 tccccaaacc agacctttga catgcggcac cataaatgag gcatttaaca acaccatggc
     5581 caagagagct gccatcacac ctacgatgaa gaatgcagag gccaagccgg cgccatcaat
     5641 caggccacct ctcggcccct cacccaagac cagaaacaac cagggaccga taaacatttg
     5701 cggaaccagc cccgccaaca tccaaccaac cccgtaccga acctgtccat cggatgggag
     5761 gaagcgtgaa ggagaggtgg agtcagtggc aaccggcccg cctgaccatc ccaacagccc
     5821 cagcagcgtc accaccaatc ccccagcagc caaaccggca aaaagaagat ggaggctttt
     5881 tggaaccact cgggtccaat ccacggtcga caatccgagt acaaaccatc ccccagccac
     5941 atgtccttca tgtgccatgg catcccatcg ggctaaccag aggacaccgg ctccaaagaa
     6001 caggacgctc gcacatccca gccagaccat ccccgaatgc ttaccgggac catttttctg
     6061 caggcttttc caaaggcaga atccgcccaa cacaaaaccc aacatttcct catagctcca
     6121 cggccccacg gcaatatgag attcaggaag agggaaacca aggatggccg gaacaagaag
     6181 tccgataact gaagacatca ccagcaaccc gatcgtcaat cgaacgacca aggagttaag
     6241 aaccacccgg agccctcttt gataggaatc gccttgccag caacacagca actccgtcac
     6301 gagcaacaga agcgtcaatc ctaacggcaa caccatccgc cacacatcca agaccgcaaa
     6361 cagcaggaac ccgtcaacca tatccgttct ctttccttaa cacatgatga gaaaaaaata
     6421 aaaaattagt caacaggcca tgccggataa acaaggatac tctcgcaggc tgttgaaaaa
     6481 ttcaaaattt cgctgagagc gttcgcaatc caaaattttg tgatctccca tgagcgaagc
     6541 aggatgttca aaaagacccg tccagaattg gcctgaggct tctcgaagaa aaggctgctt
     6601 ccgcttgaac gggcgaaacg tacagagaag tacgtgagca cggccaaggg gcgagaacgc
     6661 cgctgcggac ttttttaaca ccctgctagg gaatagaaca cgtcctggaa ggcaaagtca
     6721 tcctcatccc ttcaaacggt tttcaaaacc cgaccttagt ctggcgaggc ctggacaatc
     6781 tggaaccatc cccggttatg aagaaggtga acgggagtct caaaaaggac ggatagccgg
     6841 tgatcattca gcacatcccc cttaagacca tcttccatga tccggccctg tttcaacaac
     6901 acaacccgat caatctccgg aggaatttca tggacatggt gcgtcactaa aatcaccgtt
     6961 cctccccggt gaataaaatc tcgaatgatc tgaaggtatt gaaaacacgc ctgcacatcc
     7021 agtccggtgg ttggttcatc taacacaagg atctggggtt cgtgcaccaa agcccgacct
     7081 aacaaggctc gcctttgttc tccggtagac atctccccaa atgggcgatc tcgaagatgg
     7141 tcaatcccaa gcctgcgcat catctcagcg ctctcgttac ggtgtcggag actgatggtt
     7201 tggtggtcat acacagccat actagagaaa aaccccgaaa gaataatctc caaccctgtg
     7261 gtgtggcccg gataggcctg ttgcatatcc gaggaaatta tcccaatctg ggagcgtaat
     7321 tcccacacgt tccaatgctc cttccccatc agccggacaa atcgatccgg cccgaccacc
     7381 ggatacagtt ccctcgaaag caacttcatg aatgtcgatt tccctgaccc attgggaccc
     7441 aggat
//
LOCUS       DAORAQ010000129         7298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1568_length_7298_cov_67.950297, whole genome shotgun sequence.
ACCESSION   DAORAQ010000129 DAORAQ010000000
VERSION     DAORAQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_7298_cov_67.950297"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /gene="aspS"
                     /locus_tag="PKM72_10595"
     CDS             complement(<1..1504)
                     /gene="aspS"
                     /locus_tag="PKM72_10595"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNP61281.1"
                     /translation="MKRSHHCGELSLDHVGQTVTLAGWVASRRDHGGVRFIDLRDRYG
                     LTQIVFDSEKEPTVHQLADRLRNEYVITVTGEVTLRPEGSANPHIPTGAIEIRTTALI
                     ILNEAKPLPFALDDTITTSETLRLKHRYLDLRRPKMQELLQLRSQVSHLVRQFLHANQ
                     FIEVETPILTKSTPEGARDYLVPSRVNPGDFFALPQSPQLFKQILMIGGTDRYYQLAR
                     CFRDEDLRLDRQPEFTQIDVEMSFVDQEDIMNLIEHMVRLVFKETKSIDFPTPLPRLS
                     FEEAMGRYGTDKPDLRFDLQLQDLSQFSTTCDFKVFRSAVENGGIVKALIVPGGNAFT
                     RNRIDNLIDTAKEFGAKGLAWVKINDEGNLDSSIAKFFKTHALREALPSAKSGDLLIF
                     VADQAPIVHQVLGRLRLLLGEELQLIDRNRWEPLWVMDFPMFEYDDAAKRYVALHHPF
                     TAPHPNDIPLLETDPLKIRTQAYDLVLNGFELGGGSIRIHAPQVQSKVFDL"
     gene            1563..1706
                     /locus_tag="PKM72_10600"
     CDS             1563..1706
                     /locus_tag="PKM72_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61282.1"
                     /translation="MEVQVLNLNRLGDVTPGRRGRALLVVSGVEIVFLRLMGLEFPWR
                     GQN"
     assembly_gap    1812..1911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1912..2756)
                     /locus_tag="PKM72_10605"
     CDS             complement(<1912..2756)
                     /locus_tag="PKM72_10605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNP61283.1"
                     /translation="MAQAEKTVRLQKIIARSGFASRRKAEELIQHGLVTVNGETVMTL
                     GTKVDPAIDHIKVEGRHLKSRPPDMFLMLNKPLGYISTLHDPAGRPTIKALVPKPSIR
                     LFPVGRLDYDSEGLLLLTNNGDIAQACLHPAHHVHKTYLVKIKGILEEPEIQKLRHGL
                     MLEDGPTAPAKVKKAGKAAANSWVEITIHEGRKHQVKRMFEQIGHPVIRLKRVQFGPL
                     NLGTLPPGQTRYLTDKEANDLRHLLIAPESARLAIHQPAKRAEKIPTSRPAGQLRPRS
                     PNPNS"
     gene            complement(2759..4315)
                     /gene="guaA"
                     /locus_tag="PKM72_10610"
     CDS             complement(2759..4315)
                     /gene="guaA"
                     /locus_tag="PKM72_10610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNP61284.1"
                     /translation="MHSQHDTIVILDFGSQYTQLIARRIREFHIFSVILPSQATVQDI
                     LNYHPKGIIFSGGPASVTQAHAPKIDSQIFDLAIPILGICYGMQLLAHQCGGTVNPTP
                     HREFGRADLTIDDNTNLFLGLDLATAFPVWMSHGDSVQQLPPGFQVIAHTTHAPIAAM
                     KSSPNTQQLFGIQFHPEVIHTLHGSTILQNFARHICHCEPTWTMGSFIEQAVTNIRQT
                     VGSDKVICALSGGVDSTVAAVLAHRAIGDQLTCIFIDNGLMRKNEVAQLQTTFDSAYH
                     LKVRIADHGDTFLSALGRTTDPERKRKIIGRQFIKVFEQEAVSLGTVKFLVQGTLYPD
                     VIESLSVKGPSATIKTHHNVGGLPKRMKFKLIEPFRELFKDEVRQLGLELGLPEDIVW
                     RQPFPGPGLAIRIIGSVTAQRLAILREADAIVRDEIERAGLSRTIWQSFAVLLPIQTV
                     GVMGDQRTYESVVAIRAVTSTDGMTADWGVIPPEVLGKMSNRIINEVQGVNRVVYDIS
                     SKPPATIEWE"
     gene            complement(4375..5835)
                     /gene="guaB"
                     /locus_tag="PKM72_10615"
     CDS             complement(4375..5835)
                     /gene="guaB"
                     /locus_tag="PKM72_10615"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNP61285.1"
                     /translation="MLEKQLRIGLTFDDVVLVPRHSNIIPSDVDPSTQLTRNIRINIP
                     ILSAAMDTVTEGRLAIAIAREGGIGIIHRALSPDMQASEVDKVKKSESGMILSPITIS
                     PDHTIRDAHHLMSTYRISGIPVTKDGKLVGILTNRDLRFENRLDLKVSQVMTKNNLVT
                     APEGTGLDQAQAILHEHRIEKLPVVNDQFELKGLITIKDIQKKIKYPFACKDAHGRLR
                     VGAAVGVGPDVEERLERLTKAGIDLIVVDTAHGHSQSVLDKVKDIKSRYPDLELMAGN
                     VATSAATLDLIKAGADAIKVGVGPGSICTTRIVSGAGVPQLTAVADCATVAKEHQIPL
                     IADGGIKYSGDLTKALAAGASCVMIGSLFAGTEESPGETVLYQGRTYKEYRGMGSLGA
                     MERGGKDRYFQEGRETSKLVPEGIEARVPYKGNLAVMVYQLVGGLKAGMGYCGCRTIE
                     ELQQNAQFLQLTSAGLREAHVHDVVITKEAPNYRAD"
     gene            complement(6108..>7298)
                     /locus_tag="PKM72_10620"
     CDS             complement(6108..>7298)
                     /locus_tag="PKM72_10620"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNP61286.1"
                     /translation="AAYFISENPMTPSGTMPGWVEVEPEQKFGATIPFTTSPTDGTKT
                     VYVWFKDANNNVSVPASTHILLNTSGYICATKWGKPGRGASLLHGGEFISPLYGLTID
                     RQGSLFVVDNGNNRVQKFDRNGNFILLWGSFGAANGGFNNPTGIACDGNGNVYVADTN
                     NHRIQKFDGKLGHYMMKLGSRGNGEGQFNAPWGVAVDRVRGYLYVVDSANFRIQKFDE
                     TGEFIMQWGSFGNGDGQFYFARGIAVDQTDGTVYIVDMGNHRIQKFDTSSNVLPQLLT
                     KWGGGIGPGHASSAQAQEPGQFRSPWGVAVDESGDVFISDTGNQRIQKFDRDGNFITQ
                     WGGFGSQDGQFNFPYGIGADSRGHIYSVDSGNMRVQEFMQAEEAEDHFQEEETMAFIP
                     EAAE"
BASE COUNT         1661 a         1702 c         1879 g         1956 t
ORIGIN      
        1 gtaagtcaaa gacttttgac tgaacttgtg gcgcatgaat tcgaatactc cctcccccta
       61 actcaaagcc attcaacacc aggtcatagg cttgagttcg aatcttgagg ggatcagttt
      121 ccagaagcgg gatatcattt gggtgaggag ccgtaaacgg atggtgcagc gcgacatacc
      181 gtttggcggc atcgtcatat tcaaacatgg gaaaatccat gacccacaag ggttcccacc
      241 ggtttcggtc aatcaattga agctcctctc ccagcagtag gcgaaggcgt cctaagactt
      301 gatggacgat gggcgcttga tctgcgacaa atatgagcag gtccccggat ttggccgagg
      361 ggagcgcttc acgtagggca tgagtcttga aaaatttggc gatggaggaa tccagattgc
      421 cctcatcatt aattttgacc caggctagcc ccttggcccc gaattctttc gccgtatcaa
      481 tcagattgtc tatccggttt cgggtgaagg cgtttccccc aggaacgata agggctttaa
      541 cgatcccccc gttttccact gcactccgaa agactttaaa atcgcaggtt gtggaaaatt
      601 gactcaggtc ttggagctga agatcaaagc ggagatccgg tttgtccgtt ccatagcgtc
      661 ccatggcctc ttcaaaggac agccgaggca gcggggtggg gaaatcaatg ctcttggtct
      721 ctttaaagac aagtctgacc atatgttcga tcaggttcat gatatcttcc tgatccacga
      781 acgacatttc gacatcgatt tgcgtaaatt ctggttgtcg atccagcctg aggtcttcat
      841 cgcggaagca tcgggccagt tgataataac gatcagttcc cccaatcata agaatctgct
      901 taaagagttg cggcgattgg ggaagtgcaa aaaaatcacc tgggttgact cgactcggta
      961 cgagataatc ccgtgcgcct tccggtgtgc ttttcgttaa aatgggtgtt tccacttcta
     1021 tgaattgatt ggcgtgaagg aattgtcgca cgagatgtga cacctgactg cggagttgaa
     1081 gaagttcttg catctttggc cgtcgaaggt ccaggtaccg gtgctttaaa cgaagggtct
     1141 ctgaggtggt gatggtatcg tcaagtgcaa aggggagggg tttggcttca ttgagaatga
     1201 tgagtgctgt tgttcgtatt tctatggcac cggtcgggat gtgggggttg gctgatccct
     1261 caggtcgcag cgtcacctca cctgtgacgg tgatgacgta ctcattgcga agacgatcgg
     1321 ctagttgatg taccgtgggc tccttttctg aatcaaacac gatttgtgtg agtccgtagc
     1381 ggtcgcgaag gtcaataaac ctcactccgc catggtcgcg acggctggcc acccagcctg
     1441 ccagagtcac ggtttggcca acgtgatcca aggagagttc accgcaatga tgtgatcgtt
     1501 tcaactgtag gatcctttcc aatacctgtc agcttttgat gggagaacat gaaaccaaga
     1561 atatggaagt tcaagtttta aatttgaatc ggcttggtga tgtgacgcct ggtcggcggg
     1621 gccgtgcttt gcttgtggtg tcaggagtcg agatcgtctt cttgcgcctg atgggtttgg
     1681 agtttccctg gcgcggccaa aactgaccag ctggcccaga tttagggagc ttttccgttc
     1741 ttttgatcgt ctgatccttg gatcggtaca gccctgcttg gtcggtcgtt cccgcaggcg
     1801 ctggggtgct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natggaattc
     1921 gggtttggtg aacggggccg taactgtcct gcaggtcttg atgttgggat tttttcggcc
     1981 ctctttgccg gctggtggat agccagacgt gccgattcag gtgcgatgag taaatgccga
     2041 aggtcattcg cttccttatc tgtcaagtat cgcgtttgac ctggaggaag ggttccaagg
     2101 ttcaaaggtc caaactgaac acgttttaac cggattactg gatggccaat ttgttcaaac
     2161 atgcgtttga cttggtgttt acgtccttca tgaatggtaa tttccaccca agaattcgct
     2221 gccgcttttc cagctttttt tacttttgcg ggagctgttg gcccatcttc taacatcagt
     2281 ccatgtcgaa gtttttgaat ttctggttct tctaagatac ccttgatttt tacaagatag
     2341 gttttatgga catgatgtgc ggggtgaaga caggcttggg caatatcacc gttattggtg
     2401 agcagcagta ggccttcact atcatagtcc aaacgtccga cgggaaataa ccggatggaa
     2461 ggtttcggaa ccaaggcttt gatcgtaggg cgcccagcag gatcatgcaa cgtggagata
     2521 tatcccaaag gcttattgag catgagaaac atatcaggag ggcgggattt caggtggcgt
     2581 ccttcgacct tgatatggtc aatcgcagga tcgactttgg tccctagtgt catgaccgtt
     2641 tccccgttga cggtgaccag tccatgttgg ataagttctt cagccttgcg tctggaggcg
     2701 aagccggaac gggcgatgat tttttgtaaa cgaactgttt tctctgcctg agccatacct
     2761 attcccattc gatcgttgcc gggggttttg agctaatgtc atacaccaca cggttgaccc
     2821 cttgcacttc attgataatt cgattggaca ttttgcccaa tacctctggc ggaatcactc
     2881 cccagtccgc cgtcatgccg tctgtactgg tgacggcacg aatggcgacc actgattcgt
     2941 aggtccgttg gtcgcccatg actcccacag tctggattgg cagtaaaaca gcgaacgatt
     3001 gccagatggt tcttgacagt ccggcgcgtt caatttcatc acggacaatg gcatccgcct
     3061 cacgaagaat tgccaggcgc tgtgcggtca cagaaccgat gatgcgaatt gctaagcccg
     3121 gaccgggaaa gggttgtcgc caaacaatgt cctctggcag acctaattcc aatcccaatt
     3181 gacgaacttc atctttaaac aattctcgaa aaggttcaat cagtttaaat ttcatgcgtt
     3241 tcggtaagcc accgacatta tgatgtgttt tgatcgttgc cgagggccct ttcacgctca
     3301 gactttcaat cacatcagga tacagtgtcc cttgaacgag aaatttgact gtgcctaaag
     3361 atactgcttc ctgttcgaaa actttgatga attgacgacc aatgattttt cgtttgcgtt
     3421 ccgggtctgt tgtgcgcccc agtgctgata gaaaggtgtc tccatggtca gcaatgcgaa
     3481 ctttcagatg ataggcagaa tcgaacgtgg tttgtaattg tgccacttca ttctttcgca
     3541 taaggccatt gtcaataaag atgcaggtca attggtctcc aattgcccgg tgagccagga
     3601 cagcggccac tgtggaatcc actcccccgc taagtgcgca aatgaccttg tcgctgccga
     3661 cagtctgacg gatgtttgtc acggcttgtt cgatgaatga ccccattgtc caagttggct
     3721 cgcagtggca aatatgacgt gcaaagtttt gaagaatagt tgaaccgtgg agggtatgga
     3781 tgacttcagg gtggaactgg atcccaaaga gctgttgggt atttggggag gacttcatcg
     3841 cggcgatcgg ggcatgggtc gtgtgggcaa tcacttgaaa tcctggtgga agttgttgaa
     3901 cggaatcccc atgagacatc cacacaggaa atgcggttgc gagatcaagg ccaagaaata
     3961 aattcgtgtt gtcgtcaatg gtcagatcgg ctcgaccgaa ttcacgatgg ggtgtgggat
     4021 tgacggttcc gccacattga tgggcgagta attgcatacc gtagcagatg cccaggatgg
     4081 gtatggcaag atcaaaaatt tggctgtcta ttttgggggc atgggcctga gtaacgctgg
     4141 ccggacctcc ggaaaaaata atccctttgg ggtggtagtt aaggatgtcc tgaaccgtcg
     4201 cctgcgaggg gaggatgaca gagaaaatgt ggaattcgcg tatccgccgt gcgatgagtt
     4261 gcgtatactg cgatccaaaa tcaagaatga caatggtatc gtgttgcgag tgcatgagcc
     4321 tgcgagttaa tttgtcaacg tgggaactga ctgacttaag ggcagaacca gattttagtc
     4381 agcccgataa tttggggcct ctttggtaat caccacatca tgaacgtggg cctcccgaag
     4441 ccctgcagag gtgagctgaa ggaactgagc attctgttga agttcttcga ttgtccgaca
     4501 tccgcagtac cccattcctg ccttcagccc tccgactaat tgatagacca tgaccgcgag
     4561 atttccttta tagggaactc tggcttcaat gccttcggga actaatttgg aagtttcgcg
     4621 gccttcttga aagtagcgat cctttcctcc tcgttccatg gctcccagtg atcccatccc
     4681 ccggtattct ttgtaggttc ggccctgata caacacggtt tcacctggcg attcttcggt
     4741 tccggcaaaa agtgatccga tcattacgca agaggccccg gccgcgaggg ccttggtgag
     4801 atctcccgag tatttgatgc ctccatcagc aattaagggt atttggtgtt ctttggcgac
     4861 ggtggcacaa tcagccactg ccgttagttg agggactccg gctcctgata caattctggt
     4921 ggtgcagatt gagcctggcc cgactccaac tttaatggca tctgctccgg ccttaatgag
     4981 atccagcgta gcagcagaag ttgcgacatt tcctgccatc agctccaggt ccggatagcg
     5041 ggatttaatg tctttgactt tgtccaagac gctttgagag tgcccatgag cggtatcgac
     5101 aacgattagg tcaattccag cttttgtgag ccgttccagc cgttcttcta catcaggtcc
     5161 gaccccgacg gccgcgccaa cgcgcagacg cccgtgcgca tctttgcagg caaaaggata
     5221 tttgattttt ttctgaatgt ctttaatggt gatcaaccct tttagctcaa attgatcatt
     5281 cactaccgga agtttttcaa tgcgatgctc atgaaggatt gcctgggctt ggtctaatcc
     5341 agtgccctca ggggcagtca ctaggttgtt tttagtcata acctgggaaa ctttgagatc
     5401 cagacggttt tcaaaacgaa ggtctcgatt cgttaagatt ccaaccaatt tgccgtcttt
     5461 ggttacaggg attccggata tgcggtaggt cgacatgaga tggtgggcat cgcgaatagt
     5521 atgatccggg gagatggtaa tcggagaaag gatcatcccg ctttctgatt ttttgacctt
     5581 atcgacttct gaggcttgca tgtcagggga gagtgcgcgg tgaataattc caatccctcc
     5641 ttcacgcgct atcgcaatgg ctaatcgccc ttcggtgacg gtgtccatgg ccgcgctcag
     5701 aatggggata ttgatacgaa tatttcgagt gagttgggtg gagggatcca catcagatgg
     5761 gatgatattt gaatggcgtg ggaccaaaac cacgtcatca aaagtaagcc ctattcgtaa
     5821 ttgtttctcc aacatgagtt aggccttgta ttctgtccct tgccgaaatg tgtatgcgga
     5881 aaagtctttc atcataattt tcttttaaaa gtcagtcaat gttcttcttg ttttcccaga
     5941 tgggattggg agaacaccca ataccagtca gaatcaacag ctaactgtat tctcagccga
     6001 ggattcgggt gtggccttaa actgatgcca acgggtgcag gtattcccgt tggcatcagg
     6061 gtcaatgtgg gtgattgttt cctggggatg ttgccagagc tatgtggtta ttcggctgct
     6121 tcaggaataa aggccatcgt ttcctcttct tggaaatgat cttcggcttc ctctgcctgc
     6181 atgaattcct gaactcgcat attgccgcta tcaacggaat aaatgtgtcc tctggagtca
     6241 gcccctattc catagggaaa gttgaattgg ccatcctggg agccgaagcc accccattgg
     6301 gtgataaagt taccgtctcg atcaaacttt tggatgcgtt ggttgccggt atcactgata
     6361 aatacgtcac ctgattcgtc taccgccaca ccccagggtg atcggaattg tccaggttct
     6421 tgtgcttgag cgctgctggc atgaccaggt ccaattccgc cgccccactt ggtgagtaat
     6481 tgggggagga cgtttgagct ggtatcaaat ttttgaatcc gatgattccc catatcgact
     6541 atgtagacag ttccatcggt ttgatctacc gcaatgcctc gcgcaaaata aaactgcccg
     6601 tccccattcc caaagcttcc ccactgcatg ataaattctc cggtttcatc aaacttttga
     6661 atccgaaagt tggcactatc gacgacatac aggtacccgc ggactcggtc gaccgctaca
     6721 ccccatggag cattaaactg tccttcgcca ttaccacggg atccgagttt catcatgtaa
     6781 tgccctaatt tcccatcaaa tttttgaatc cgatggttat tggtgtctgc cacatataca
     6841 ttgccattcc catcacatgc gataccggtt ggattattga aaccgccatt agcggcaccg
     6901 aaacttcccc ataggaggat gaaattgcca ttacggtcga atttctgaac tcggttgttc
     6961 ccgttatcaa ccacaaataa tgacccttgg cgatcaatcg tcagcccgta tagagggcta
     7021 ataaattctc ctccatggag gagggaggct ccccggcctg gttttcccca ctttgtggcg
     7081 caaatatacc cagatgtatt gagaaggata tgggtgctgg ccggaacgga aacgttattg
     7141 ttggcatctt taaaccaaac gtagacggtt tttgttccat ctgttggaga ggtggtaaaa
     7201 ggaatcgtgg ctccaaattt ttgttccggc tccacctcaa cccacccagg catggtcccg
     7261 cttggagtca tggggttttc agagatgaaa taggcagc
//
LOCUS       DAORAQ010000130         7257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1576_length_7257_cov_60.191752, whole genome shotgun sequence.
ACCESSION   DAORAQ010000130 DAORAQ010000000
VERSION     DAORAQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1576_length_7257_cov_60.191752"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..817)
                     /locus_tag="PKM72_10625"
     CDS             complement(89..817)
                     /locus_tag="PKM72_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61287.1"
                     /translation="MNSPKDSERSYLLRFKELCPEFPDGSIEDSESPDFLVHGSHGTV
                     GIELTAVYRPDPIDKNAHLPAIEKEREKIVEKIKHYYIHIEDVPLRVSFLFSGNVTNK
                     SDIKNEDLKEMATLIHSFKPQIGETVYLDIENNLPFWVDRVSITTREPDGQNFWTAQK
                     CVWIPELSPIEIQREINKKEEKHISYLEKCSFAWLVLMSQVGEGLGTHVKFSNGVWSH
                     RYETLFEQVWIFQNPNCARKLEVV"
     gene            complement(1131..1550)
                     /locus_tag="PKM72_10630"
     CDS             complement(1131..1550)
                     /locus_tag="PKM72_10630"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNP61288.1"
                     /translation="MGAKPRFVIYAHNATYDKLHQVATLGLTAAAMGKDVMVVLLFWT
                     IKKLAEGRINELDFPPEYAAQAPEVARLIRERKVPLISEMFEEARHVGQFKLVACSAG
                     LEYMGVDAKAVTQGVDEVMGLPAIISACSGAETTLFI"
     gene            complement(1649..2374)
                     /locus_tag="PKM72_10635"
     CDS             complement(1649..2374)
                     /locus_tag="PKM72_10635"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HNP61289.1"
                     /translation="MVQESEDQKEFIEEVAVVGVKVRDLGEVKKTRTDDHTVKVGDWV
                     LLDLDNDHTFGKVYLPPQFLPFSPPMRVMRQIIRKATEDDKRQIARQVRLAQEGEEFC
                     NQRIEESGLKMTLVEVFGSFAQRSLTFTYTADDRIDFRQLVKDLARRFGCRIEMRQIS
                     SREEAARLSGVDTCGLVLCCSSFLTDFKPVYPRGRADAPMNGMDSHRIGVCGRMKCCL
                     LYEEEGWTLTRRKPQPLIHPTKR"
     gene            complement(2511..4130)
                     /locus_tag="PKM72_10640"
     CDS             complement(2511..4130)
                     /locus_tag="PKM72_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP61290.1"
                     /translation="MNPWTNINPTQYVGLHPWVWVQLESAEEPGPFPFLGGVDPEVVN
                     SLHQVHGLMMSGIETAISDIMAKRASVDDPQISRRLEDAYAEVVQSRPLLQRHIQCGR
                     KSDGTFHWTYPRNPTASAKMTYGGMRIFNSVTRQAIPFGFERPIGPNVGHFLGFLTGR
                     NTMGEIQTVAQAGGRDLERQLAQLFTLLKDHDCLATAPNTSIEAHWRQITKDQDVVHL
                     GHAALMYRHQDSFLWFDPWLMPWFAESPVPSLWANLLPRPAGIFLTHDHDDHVDPRTL
                     YHLPKDVPIFVPSRRNRKTLHYDYLSLLRELGFSQVRELAHGESWRIGDAEVVSVPFF
                     GEDPCDVELPRNCYLVADRGRNTLVHADSGPTNDGHSALQDKVMAKLVSKYGPIPLVF
                     ASQQQLKEVRSYAAYACLSPPGTWLEVGENGFLTNAYLAELCRTAQAKLFVSYATGGA
                     DWYPDHLSFMFSGRNPARTALLTANWDPPESLKQELEPFGCRYHFGQAFDVFGAGSDG
                     ETLVSHLSDQLAPLGLYQLDHAPPPFLRKQR"
     gene            complement(4206..4613)
                     /locus_tag="PKM72_10645"
     CDS             complement(4206..4613)
                     /locus_tag="PKM72_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNP61291.1"
                     /translation="MQEKRKTRGLRHLALRVRDVQVSQRFYERLFEMKVVWHPDPENV
                     YLSTGYDNLALHQVTAEELSQFNLSRVHPLDHLGFIMDSPSSVDALFSEATEQGATIV
                     KPLKQHRDGSYSFYLSDPDFNTIQVLFEPSIQL"
     gene            4821..5738
                     /locus_tag="PKM72_10650"
     CDS             4821..5738
                     /locus_tag="PKM72_10650"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNP61292.1"
                     /translation="MNEIYTKLWHGQKELEEKMAGMMRNRRIGYIGVGACLLFIAGAS
                     SMVSASETPQEIVGKDGAPMVLIPEGIFPMGVPKAARDGGLDERPNHDVFVSTFYMDK
                     YELTNGRYLQFVTETGHRTPQHPTDPKRGLWKQNMMPESVTDLPVINVDWKDAEAYCH
                     WAGKRLPTEAEWEKAAKGPNDWRFPWGDVEPTLDHLNFNQSWRGEATLTQVGIYEKGK
                     SPYGIYDVAGNVWEWVADWYEADYYSKSPARNPPGPETGTYKVLRSSGWQGETPQVRI
                     FTRIKSLPTDRNNSTGFRCAKDGPDPSTH"
     gene            5767..6651
                     /locus_tag="PKM72_10655"
     CDS             5767..6651
                     /locus_tag="PKM72_10655"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNP61293.1"
                     /translation="MNGKQSNAGFQGLRVGALESRMAKEMERLIVRHGGVPLVAPSMR
                     ELPLSDNPQALECGDALLAGHVDILVLLTGVGFRTLLDVVQTRYALEAITGALRNTVL
                     VVRGPKPALVLKDLGLQPNILVPEPNTWKDTLVAIDASYPDGLKGLRIAVQEYGVSNP
                     KFLEGLRERGAKVRPVPVYRWTLPEDLAPLKHLLQEVMDGVIPVLLITNAIQVEHLIK
                     VLEKDEKINEFRKALHRMMVASIGHLATERLRHYGFPVDLEPSHPKMGILVKEASQAV
                     HTILTTKNAPPMRDTPAP"
BASE COUNT         1900 a         1917 c         1734 g         1706 t
ORIGIN      
        1 catgacagga accacctcaa ggacctagca cccgatctca agttttgtca agaatcaagg
       61 gttagagcgt gtctttctgg gagttgtctt aaacaacttc caattttctt gcacaattgg
      121 gattttggaa tatccagacc tgctcaaaga gagtttcata cctatggctc cacacaccat
      181 tcgaaaattt gacatgggtt cccaaacctt ctccaacctg actcatcaga actaaccatg
      241 caaaagaaca tttctctagg taagaaatgt gtttttcttc ctttttattg atctctcttt
      301 ggatttcaat tggagataat tcaggaatcc aaacacattt ttgggcggtc caaaaattct
      361 gtccgtccgg ttctcgagta gttatggaaa ctcgatcaac ccaaaatgga agattgttct
      421 caatatccag atatactgtc tcaccaatct gtggcttaaa tgaatgtatc agagtggcca
      481 tttccttcag atcctcattt tttatgtcac ttttattcgt aacattgcca ctgaataaga
      541 aacttactct tagggggaca tcctcgatat gaatgtaata atgttttatt ttttcaacga
      601 ttttttcacg ctctttttca atcgctggaa gatgcgcatt tttgtcaatt gggtcaggtc
      661 tgtacaccgc agttaattct attcctacgg tgccatgtga accgtgtact aaaaagtctg
      721 ggctttctga gtcctcaata cttccatcag ggaactctgg gcacagctcc ttaaatctca
      781 acagataaga tctttctgaa tctttggggg aattcattga ccctccatcg gccaccagac
      841 ggacgcctgc cgttctgccc tttaggtgga tgctctgatc aaatggcggg ccttgtttga
      901 gctcagcgag ttggaccgcc ttccttcatg gcctgcatcc acctaatttg atgaggctgg
      961 acggggcgtc actggttttg ggtccttttg ccgaaacaaa aggatctcgg ctgccgggcc
     1021 gaaacccggc acctctcggc catccagtga aagcctacac tttttcgcta ggaataaagc
     1081 agaggtttat tgaaatgact aaactttata ataaaaaaat ctgatgaaag ctatataaat
     1141 aaggtggtct cagcacccga gcaggcagag ataatcgcag gcaaccccat cacctcatca
     1201 accccctgag tgactgcttt ggcatcaacc cccatatact ccagtcctgc gctacaggcg
     1261 accagtttaa attggccaac gtgacgggct tcctcgaaca tttccgaaat gagtgggact
     1321 ttgcgttcac ggatcaaccg ggccacttcg ggggcttgag cggcatactc cggaggaaaa
     1381 tccaattcat ttatccggcc ttccgccaac tttttgatgg tccagaacaa caaaaccacc
     1441 atcacgtctt tccccatcgc cgccgccgtt aaccccagcg tcgccacttg atgcaactta
     1501 tcataggtcg cattatgtgc ataaatgacg aatcgtggct tagcccccat cttaaatcgt
     1561 atctccgctt atcctcatac ccagccaagc ttgccgatgc gacatcgcac tccttctcgc
     1621 tacttccact attctgacga acaggttgtt atcgttttgt gggatgaatc aacggctgtg
     1681 gcttgcgtcg agtgagtgtc caaccctctt cctcatataa caaacaacat ttcatccgac
     1741 cacagacccc gatccggtgg ctatccatac cattcatggg ggcatctgcc cgtccccgag
     1801 gatagaccgg cttaaaatca gtcaaaaagc tggagcaaca caacaccaat ccacaggtat
     1861 ccacaccgct cagtctggcg gcttcctccc ggcttgaaat ctggcgcatc tcaatccgac
     1921 atccaaatcg cctggctaga tctttgacca gttgccgaaa atcaatccga tcatcggccg
     1981 tataggtaaa agtaagtgat cgctgggcaa acgatccaaa cacctccacc aaggtcattt
     2041 tgaggccgga ttcttcaatt cgctgattac aaaattcctc accttcctgg gccaatcgga
     2101 cctggcgggc aatctgacgt ttatcatctt cagtggcttt tcgaataatc tgccgcatca
     2161 cccgcattgg cgggctaaat ggaagaaatt gaggagggag atagactttc ccaaaagtat
     2221 ggtcgttatc taaatccaac aaaacccaat cgccgacttt cactgtatga tcatcggtcc
     2281 tggttttttt aacctcccct agatcccgaa ctttgacgcc gaccaccgca acttcttcta
     2341 taaattcttt ttggtcttca gattcctgca ccatgaatca ttgtctccct ttgctaccta
     2401 ggaatactgt aggcgtgagg tccaatcata ccggacttgc tggaagaggc caagcgggaa
     2461 tcctggaggc tcaggcctcg tgacttcacg ccacccctcc catgccatgg ctaccgttgc
     2521 tttcgcagga agggaggagg agcatgatcc aactgataaa gcccgagtgg tgccagctga
     2581 tctgacagat gagataccag ggtttctcca tcggatccgg ctccaaacac atcaaacgcc
     2641 tggccgaaat gatagcgaca gccaaacggt tccagttcct gcttaagact ttcaggagga
     2701 tcccaattcg cggtgagcag ggccgtccgg gccggattac gcccactgaa cataaacgat
     2761 aaatgatcgg gataccaatc cgcacctcct gtcgcatagg agacaaagag tttggcttga
     2821 gccgttcgac acaactcggc taaataggca ttggtcaaaa agccgttttc tcccacctcc
     2881 aaccaagttc ccggaggaga gaggcaggca taggccgcat agctccggac ttctttgagt
     2941 tgttgttgcg aggcaaaaac caagggaatc ggaccatact ttgacacgag cttggccatg
     3001 accttgtctt gcaatgccga gtggccatca ttggtcggcc cgctatcggc atgcaccaac
     3061 gtattccgcc cacggtccgc caccaaataa caatttcgag gaagttccac atcacatggg
     3121 tcttcaccaa aaaacggcac ggacacgacc tcggcatctc ctatacgcca actttcgcca
     3181 tgggccagct ccctgacctg tgaaaaacct agttccctga gaagggacag ataatcataa
     3241 tgcagcgttt tccggttgcg tcgactcgga acaaaaatcg gcacatcttt gggtaaatgg
     3301 tacagagttc ggggatcgac atggtcgtca tgatcatgag tgagaaaaat tccagccggg
     3361 cggggcagga gattggccca cagagatggg acaggcgact ccgcgaacca tggcatgagc
     3421 cagggatcaa accataaaaa actatcctga tgtcgataca tcaacgcggc atgacccaga
     3481 tgcaccacat cctggtcctt tgtgatttgc ctccaatggg cctcaatgga ggtgtttggc
     3541 gcagtggcga gacaatcatg gtctttcaag agggtaaaga gctgcgccaa ttgacgctca
     3601 aggtctcgcc cgccagcctg ggccaccgtt tgaatttcgc ccatcgtatt cctgccagtg
     3661 agaaaaccca ggaaatgccc tacgttcggc ccgattggcc gctcaaaccc aaaggggatg
     3721 gcttgtcgcg tgacggaatt aaaaatccgc attcccccgt atgtcatttt ggctgaggca
     3781 gtaggatttc ttggataagt ccagtgaaag gtcccatcgg atttccgtcc gcattggata
     3841 tgtcgttgca ggagaggacg gctttgcacc acttccgcat aggcatcctc caaacgacgg
     3901 gatatctgag ggtcatccac agaagctcgt ttggccatga tgtcgctgat ggcggtctcg
     3961 ataccgctca tcatgagccc atgcacctga tgaagactgt tgacaacttc cggatccacc
     4021 ccgcccaaaa acggaaatgg tccgggctct tccgcacttt ccaactgaac ccacacccag
     4081 gggtgcaggc cgacatactg cgtgggatta atgttcgtcc agggattcat gacaacgtga
     4141 gatggagaaa aaactctgtc gtatatggtg aaaagaagga accccggggg ttcgaccctt
     4201 ctctatcaca attggattga gggttcaaac aacacctgga ttgtattgaa atccggatcc
     4261 gagagataaa acgaataact gccatcacga tgttgcttca ggggtttcac aatcgtggcg
     4321 ccctgttccg tggcttcgga aaacagcgca tccacacttg acggagaatc catgataaac
     4381 cccaaatgat ccaacgggtg aacccgggaa aggttaaatt gacttaactc ttcagccgtc
     4441 acttgatgca gcgccagatt gtcatagccc gtactgagat aaacattttc gggatcagga
     4501 tgccacacaa ctttcatctc aaacagtcgt tcataaaacc gttgggaaac ctgaacatcc
     4561 cgcacccgca aggccaaatg tcgaaggcca cgggtttttc ttttttcctg catctcaatt
     4621 ccgtgaatgg ataaagaggg aaaccgcatc actcaagagt attttcggag aaaggcatca
     4681 taaccaggac actggaaggg agcaagttgc caaacaaatg ccgaaggttc ctaaggtctg
     4741 ttaaaaatct taccctgttc aactttccct cctaaaacct ttggactatc attcatgatc
     4801 agggggattt atggtatttt atgaatgaaa tatatacaaa attgtggcat ggtcagaaag
     4861 aattggagga aaagatggca ggaatgatgc ggaatcgacg gatagggtat atcggggtag
     4921 gagcgtgcct actcttcatc gctggagctt ccagcatggt gagcgcttcc gagacccctc
     4981 aggaaattgt cggaaaagac ggcgctccca tggtcttgat tcccgaagga atcttcccga
     5041 tgggagtccc taaagcggcc agggatgggg gactcgacga acggccgaat catgacgtgt
     5101 tcgtgagtac cttttatatg gataagtatg aactcaccaa tggacggtat cttcaattcg
     5161 tgaccgaaac cgggcaccgc actccccaac atcccacgga tccgaaaagg ggtctgtgga
     5221 aacagaatat gatgcccgag tcggttaccg accttccggt tatcaacgtg gactggaaag
     5281 atgccgaagc ctattgccac tgggcaggaa aacgtcttcc caccgaagcc gaatgggaaa
     5341 aagcggcaaa aggccccaac gactggcgct ttccttgggg tgacgtcgaa cccacgctgg
     5401 accatttgaa tttcaatcaa tcgtggcgcg gagaggccac attaacccag gtgggtattt
     5461 atgaaaaagg caaaagcccc tatggtattt acgatgtagc tggcaatgtc tgggaatggg
     5521 tggctgactg gtacgaagca gactattaca gcaagagccc tgcacggaat cccccaggac
     5581 ccgaaaccgg cacatacaaa gtgttgcgga gttcaggctg gcaaggggag acaccccagg
     5641 tgcgaatctt cacgaggatc aaaagtcttc caacggaccg taataattcc acggggttcc
     5701 gatgcgccaa ggatgggcct gatccatcaa cccactaaac cttacaaccc gttgaaagta
     5761 ccacccatga acggtaaaca atccaatgcc ggctttcaag gactccgggt cggggctctt
     5821 gaaagccgga tggctaagga aatggaacgg ttgattgtcc ggcacggagg agtgcccctc
     5881 gtcgccccgt caatgaggga actccctcta tcggacaatc ctcaggcgct ggaatgtggc
     5941 gatgccttgc tggctggtca tgtcgacata cttgtcctgc tcaccggagt aggatttcgc
     6001 actctgttgg atgtcgttca aacccgatat gcactggagg caatcaccgg cgccttgcgc
     6061 aacacggtgc tcgtcgtgcg gggtcccaaa ccagccctgg tattaaaaga ccttggactt
     6121 cagccgaata tcctggtccc cgaacccaat acatggaaag atactctggt tgccattgat
     6181 gcatcctacc cagacggact caagggctta cggattgcag tgcaggaata tggggtcagt
     6241 aacccgaaat ttttagaagg cctgagggaa cggggagcaa aagtccgacc ggttccagtc
     6301 tatcggtgga ccttacctga ggatctggca cccctgaaac atctgcttca agaagtcatg
     6361 gatggggtca taccggtttt gttgatcacc aacgccatcc aagttgaaca tctcatcaag
     6421 gtgctagaaa aggatgaaaa gatcaatgag ttccgaaagg cccttcatcg catgatggtc
     6481 gcctccatcg gccatctggc gactgaacgt ttacgccact atggattccc cgtcgacctc
     6541 gaaccctccc accccaaaat ggggattctg gtcaaagaag cctcccaggc cgttcacacc
     6601 atcctgacca cgaagaacgc cccgccaatg agggatacac ccgcgccctg atcctcagcg
     6661 aacatatcag acatttaccc ttataaccgg aataggcatt ttctttaact tccgactggg
     6721 tcggagggaa atgtaccatc atttcaagca cagtccacta actggaattt ttagaattca
     6781 tggtagttgc cgggtttcgg cccggcagcc gattcacttt tgttttggca aaagtaaaca
     6841 aaaccattga cgccccgtcc ggtctcatta agatgggacg gacgcgagcc tgaggagggc
     6901 ggcctaactc gcggagcttg tcctgagcca ggccgaaggg ctcaaacaaa gcccgccggt
     6961 ccatgagagc gtccgaccca aggaccggac ggcaggcgtc agtcaatggg gaggggccat
     7021 agacacaaaa gactatggaa atttatcgta atcaccattc atttgggagt cttttttttg
     7081 acaggaaaag gccccctaag tggtcggctt tagacagcac cagctcaatg aagacgctag
     7141 cgccattttt taggatcgcg agcggtgtga aatatgccgt agaccgtcac ttgatcttca
     7201 aaatactcat agaagatcac atacgggaag gtaagcgtcg caccaaccca cgacgat
//
LOCUS       DAORAQ010000131         7076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1615_length_7076_cov_66.667426, whole genome shotgun sequence.
ACCESSION   DAORAQ010000131 DAORAQ010000000
VERSION     DAORAQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1615_length_7076_cov_66.667426"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKM72_10660"
     CDS             <1..381
                     /locus_tag="PKM72_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNP61294.1"
                     /translation="QETYRLIQEVGYQAAFVFKYSERKHTIAARKFVDDVPEQVKGAR
                     TNQIVELQKEISLSKNKELIGTTVEVMLEGHSKKSDLQWMGHSDHNVTVVWPKDNEVR
                     QPGDLVPVLVSDASPSTLYGHVQS"
     gene            777..1736
                     /locus_tag="PKM72_10665"
     CDS             777..1736
                     /locus_tag="PKM72_10665"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP61295.1"
                     /translation="MDAQILQRIKNCKTLPAIPALPLQVLQLCRDERTTAQRIGDVIS
                     KDPSFVAKLLNVANSSFYGGSRHKVTTVTHAVTLLGMNSIATLAFCFSLYRDLRKKGG
                     GAFDHTHFWHRCILASLASKVLAKRVRVTNEEEIFLAGLLQDLGVLVMSEALGMEYGL
                     IYKKAAQDHQVLETLEQDRWKTDHCEIGAWLAETWELPELLRESVKGSHDPSLASSSD
                     EVSRLTIQCVALSGRLADMWCHQQPEMAIQAAIQSSKELLDIEPEGLVEVAKQIADGI
                     PDMSSFFQISLGNSDDIQKVLDNLEQIVTGISPSEMSEPIPAL"
     gene            complement(1950..2462)
                     /locus_tag="PKM72_10670"
     CDS             complement(1950..2462)
                     /locus_tag="PKM72_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61296.1"
                     /translation="MANTYHLWLAPTGRAYDILMEAITDLSRAYHAPVFEPHVTLLGS
                     LPSTEEDISVRCLELGESLSPIDILLTEPGLADQYFQCVFLKTQETTALMNAHELARK
                     MFVKDPSPYMPHLSLLYGHYSLELKDRITSTLSQNLRINFTSDMIHLIRSKSKHPVDW
                     TSILTVPLSG"
     gene            complement(2527..3273)
                     /locus_tag="PKM72_10675"
     CDS             complement(2527..3273)
                     /locus_tag="PKM72_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61297.1"
                     /translation="MSERKDRQNIVMPDGISFLPPIPVGYEVYYENLEIQGIQDRQTI
                     VWQFIQGSNHLLDWEQEPSNPQDPHAIKIFGVSEQIFSTKRDMLGYVPGDVAKQIVTG
                     GIWPFLQLRLDYISVANQGFIAIRFQILGPIRNTTGDEKTLSGPGKKGDRRNTQATRP
                     TLLFGREAEPPKAYRSKFKQFAFFILRIILILLGTALAFVGGTMGTAAWQAKGNLFVL
                     MLVPLALIMSGGLLIWTGIKPSKPKEKIPT"
     gene            complement(4216..5640)
                     /locus_tag="PKM72_10680"
     CDS             complement(4216..5640)
                     /locus_tag="PKM72_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNP61298.1"
                     /translation="MKRKNWTYLPVILGLANGLLFLPLAIESLHAGTVPDIRGTWTDG
                     PSSSSTTGCQDPDDNGPDNDPGGDTYTITNQTGANWSLTQVDTVVDNGFSAVQTVTCS
                     GSVSPDGTVSASCPYVVTLNGGFWFSGTSTLTGSLAGNTLTYSFAGRDLVGDTCQWTQ
                     TGTDTRSGPVPPPIMLPTNSPYDLNGDGKADLLWRNATTGSTAIWLMNGTVRTSVGFP
                     GGVPLNWQIAGIGDVTGDGKADVIWRNSTNGTVAIWLMNGATVTSTGFPGSAPTTWVI
                     QAVGDVNGDGKADLIWRNSSDGNTAIWLMNGTLRTAVGFPGGVPLNWQIKWVGDVTGD
                     GKADVIWCNGSNGAVAVWVMNGTIVTNTGFPGSTSTDWKIAGMGDFNGDGKADLIWKN
                     DTSDIVSIWLMNGTSIASSKVLGGIASAWEIEQVGDMSGDGKADVVLKNTTTGEVKVW
                     LMNGVTLIGISSPDTVSTDWDIQP"
     gene            complement(6004..6390)
                     /locus_tag="PKM72_10685"
     CDS             complement(6004..6390)
                     /locus_tag="PKM72_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61299.1"
                     /translation="MVEDDPSVAGLLKTVLEDSEYHVYVAGNGREGLDVVMNYRVDGI
                     LLDMHMPVMNGRTMLDELRWLEYQIPVLAMSDALDLPALRQLLSEGAQGFFLKPCNLQ
                     SLKQSCHQIFKNDGMSGSFLQPFHTG"
     gene            complement(6802..>7076)
                     /locus_tag="PKM72_10690"
     CDS             complement(6802..>7076)
                     /locus_tag="PKM72_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP61300.1"
                     /translation="GLEVAILGAKEMGCQHIILNFAEITWIDSMGLGQLFLWYHKMRP
                     NHVHLSIVSPQPTVKYLLESTHLSEIVPIYQSEKEAVEAQPQTSLP"
BASE COUNT         1795 a         1658 c         1741 g         1882 t
ORIGIN      
        1 caggagacct atcgtcttat ccaagaggtg ggatatcagg cggcattcgt atttaaatat
       61 tctgaacgaa agcacacgat tgcggccaga aaatttgtgg atgatgtccc tgagcaggtg
      121 aagggggctc ggacgaatca gatcgtcgaa ttgcaaaaag aaatttccct ttcaaaaaac
      181 aaagaactca tcggcaccac ggtagaggtc atgttggaag ggcattccaa aaaatccgat
      241 ttgcaatgga tggggcatag cgatcacaat gtgacggtgg tctggcccaa ggataacgaa
      301 gtccgtcaac caggagatct ggtgcctgtt ttggtcagtg atgcttcgcc ttccaccctt
      361 tatggacatg tccaatcctg atggattttg tgtgtttctg gtaacccaag attctttatc
      421 ccatcctcat tccctaaatc gctgattttt ctgcgattcc ttttgtgtga atcctcccaa
      481 acagaaattt tactgagttt gccgtcgtgt ttggttggga gaatccggcc caatcgtacg
      541 ctggtttcta cctcaagtca gttgctcatt gtccgataaa acctttgatt acttgtagat
      601 ttccggtcgc atggcggaac ggaatgtctt tcaacttcct ggtgctcatt tagaaaaaac
      661 tccccataca aacgagctat ggaaaccagc ctatatcgtg ttacgtctgg atgattggct
      721 ttcgaaggaa tgttgttgtg atggatggcg ataaaaggcc tgaggtgtag cgaacaatgg
      781 atgcccagat tctgcagcgt ataaaaaatt gcaagaccct tccggcgatt cccgcgctgc
      841 ctctgcaagt acttcaactg tgtcgggatg agaggactac cgctcaacgt attggtgacg
      901 tcattagtaa agatccatcc tttgtcgcca aactgttgaa tgtggcaaat tccagttttt
      961 acgggggcag tcgccataaa gtgacgacgg tcacccatgc ggtgacgtta ttgggcatga
     1021 attctattgc cacgttagcc ttttgttttt cgttatatcg ggatttacga aaaaaaggtg
     1081 gcggagcgtt tgatcatacc catttttggc atcgatgtat tttggcgagt ttggcctcga
     1141 aagtattggc caagcgcgtc cgggtgacga acgaagaaga aattttcctt gccggattat
     1201 tgcaggactt gggcgtgttg gtgatgagcg aggctttagg catggagtat gggttgatat
     1261 ataaaaaagc tgcgcaggat catcaggtat tggagacgtt agaacaggat cgatggaaaa
     1321 cggatcattg cgagatcggg gcatggttgg ctgagacctg ggaattgccc gaattattgc
     1381 gagaatccgt caaagggagt catgatccct ccttggcgtc cagtagcgat gaagtttccc
     1441 ggttaactat ccaatgtgta gccctttcgg ggcgtttagc tgatatgtgg tgccaccaac
     1501 agccggagat ggctattcaa gcagctatcc aatcatcaaa ggagcttttg gatattgaac
     1561 ctgagggact tgtggaagtc gccaaacaaa ttgctgatgg aattcctgat atgtccagct
     1621 ttttccaaat cagcttgggg aactccgatg acattcaaaa agtgttggat aatcttgagc
     1681 aaatcgtcac gggtatctct ccatccgaaa tgtcagaacc aatccctgcc ttataaggcc
     1741 gggattcctc caaggtttct ccgcagtcta cctcagcatt caatcagttt agctccagtt
     1801 ttatgcgaat gtatttgccc gtgacttcca atgtcaggtg gatggcggga taagtccgtc
     1861 gaattaccag gaaatgccga agacctgacc taggaggtct tgagacataa ggcgacattc
     1921 actcgggatc gtgtagaggg aaagagaatt tatccgctca aggggactgt cagaattgag
     1981 gtccagtcaa cgggatgttt gcttttggag cggataagat ggatcatgtc ggatgtaaag
     2041 ttgatgcgca aattttgaga taaagttgac gtgattctat ctttcagttc aagggaataa
     2101 tgaccataca acaggctgag atggggcata taggggctgg gatccttgac gaacattttt
     2161 cttgccagtt catgcgcatt catgagtgct gttgtttcct gggtctttaa gaacacacat
     2221 tgaaaatact gatcagcaag gcctggttcg gtcagaagaa tatcgatagg tgaaagcgat
     2281 tcaccaagtt ccaagcaccg aacggatata tcctcttctg tgcttggaag agaacccaag
     2341 agcgtcacat gcggctcgaa gactggagcg tgatacgctc tgctcaaatc ggtaatggcc
     2401 tccatgagaa tgtcgtaggc ccgaccagta ggtgccaacc agaggtgata ggtgtttgcc
     2461 acatacacct ttcagatttg ttgagaagcg tcggaataac gcatttttat ggcccaaagg
     2521 agtattttaa gtagggattt tttcttttgg ttttgaaggt ttaattccgg tccagatgag
     2581 caggcctcca ctcatgatca atgccaatgg aacgagcatc aatacaaaga ggttcccctt
     2641 ggcttgccag gctgctgtgc ccattgtccc gccgacaaaa gccaacgcgg tgccgagtaa
     2701 gatcaggata atccttagga tgaagaatgc gaactgcttg aacttgcttc gatacgcttt
     2761 tggaggttct gcctctctac cgaacaaaag tgttggtctc gtggcctggg tgttccgtcg
     2821 gtcccccttt ttgcccggtc cactaagtgt tttctcgtca ccggttgtgt tcctgatggg
     2881 cccaaggatt tgaaaccgga tggcaatgaa cccttgattt gcgacagaga tgtaatccag
     2941 cctgagttgc aaaaatggcc agatcccccc agtcacaatc tgtttggcta catcacccgg
     3001 aacatacccc aacatatccc gttttgtaga aaagatttgc tccgataccc caaatatttt
     3061 gatggcatga gggtcctggg gattgctcgg ttcttgttcc cagtccaaca gatgattgga
     3121 accttggata aactgccaga caatggtctg ccgatcttga attccctgaa tctcaaggtt
     3181 ttcatagtag acttcatacc cgactgggat tggtggcaag aaagaaatgc catcaggcat
     3241 gacgatgttc tgccggtctt ttcgttccga catgcttcag tcgtaccttt ttcgttgatt
     3301 taagggggag aaatggtgac ttctctctcg aaacaatgtc ttttggtcta tttctttcaa
     3361 cagggtcctc tcttgggtgt gctgcataga gcttgaacca actccgatca agcaacggtc
     3421 catacaccat acaaaaattc gagggtaaag gaaatgcatg gaaagatttg tagctaattg
     3481 acatgttgtc ttggtgcggt gttgttgctt gccccaacag aagagcgggg aatgacctcc
     3541 aaaatattgg ccaactagga gatgtagagg tgatcaaaca ggaggtctct ttcctattcc
     3601 gtttcataag gaaaaggaga agggtgccat gttgaatttg aaaggagagc tatgaatatg
     3661 aggagggact attgagtcaa agaggaaaga attggttgta ggtccgatct ttttactttc
     3721 attattggga ttggaaaaga gttgctcaca tgaaaaagac tcatcatgta aaatgaaggt
     3781 aaactatgaa gcctttagca ataggccggg agtttaatgg cgtcaagaaa gataggattt
     3841 tcattgaccg tgaggattat tttacaatcc acacaagaat cgcggcaaag agaatgagcc
     3901 accaccgaac actcaacgag aacttttctc cctcgctttg gtagtaaaat cgtctattca
     3961 tctcacatct cctcagttga tgattttcaa aatacagggg tatgttgatg ctgaccatta
     4021 cacatccgac ctacattcga ggccgggtct gttgggggat gggcctttcc caatgagcaa
     4081 ttgggtcaaa caaaaaagag cctgcccgat gcggggcagg ctctgggaaa agtacctgaa
     4141 gttaactttg ttttttttgc ctgagatttc ttctcttatc cttcctcaat gtttcagtga
     4201 agggttttca ccactttagg gttgaatgtc ccagtcggtg gagactgtgt caggggagct
     4261 gattccaatc agggttaccc cattcatcag ccagactttc acctcacctg tcgttgtatt
     4321 cttcaagacc acgtccgctt tcccatctcc actcatatcc cccacctgct caatctccca
     4381 cgccgaggcg atgccgccta aaactttaga cgacgcaata gaggtcccat tcatcagcca
     4441 gatggacacg atgtcgcttg tgtcattctt ccagataagg tcggccttcc catcgccatt
     4501 gaaatcgccc ataccggcaa tcttccaatc cgtggaggtg ctgccgggaa agcccgtgtt
     4561 ggtcactatg gttccattca tcacccacac tgccacggct ccattgctgc cgttacacca
     4621 tatgacatcg gccttgccat ccccagtcac atctccgacc cacttgattt gccagttgag
     4681 cggcaccccg cccgggaagc cgacggcggt ccggagagtc ccattcatca gccagatggc
     4741 cgtgttaccg tcactgctgt tacgccatat caaatcggct ttgccatcgc cgttgacgtc
     4801 cccgacggcc tgaatgaccc aggtggtcgg cgcactgccg ggaaaacccg tggacgtgac
     4861 ggtcgcgccg ttcatcagcc agatcgccac cgtgccgttg gtgctattgc gccagatgac
     4921 atcggccttg ccatcccctg ttacatcgcc gattcccgcg atctgccagt tgagcggcac
     4981 cccacccggg aagccgaccg aggtccggac ggtcccattc atcagccaga tggccgtgct
     5041 cccggtagtc gcgttacgcc ataataaatc ggctttcccg tccccattga gatcatatgg
     5101 ggaattcgtg ggcaacatga ttggaggagg gactggtcca ctcctggtat ctgttcccgt
     5161 ctgagtccac tgacaggtat caccaacgag atcccgacca gcaaacgagt aggtcaaggt
     5221 attgcccgca agggaacccg ttaaagtgga tgtgccgcta aaccaaaacc cgccgttgag
     5281 ggtaaccaca taggggcatg aggcgctcac cgtcccatca ggtgaaacgg agcctgagca
     5341 ggtcactgtt tgtacagctg aaaatccgtt atcaaccacg gtatccacct gtgtgaggct
     5401 ccaattggcg ccagtttgat tggtaatagt gtaagtatcc cctccaggat cgttgtcggg
     5461 accgttatca tctggatcct gacatcccgt tgttgagctg ctactggggc catctgtcca
     5521 ggtgcctcga atatccggca ctgtccccgc atgcaggctc tctatggcca aaggcagaaa
     5581 aagtaatcca tttgctaaac ccaggatcac cggtaaatat gtccaatttt tgcgcttcat
     5641 gtgaaatgcc tcctgttgaa tccctgacag ttgctttcca ccgttgagtc tgaaataagc
     5701 aaaaaataaa ccttttgtag gacacaattc tcatgggcgt taatctcttg tagaaatgca
     5761 cccttgtaga atggggcgca tgaaaagact acctgtggca cagggagcct ttgtagaaaa
     5821 aatgtccaaa ccatcatgcc actttgtcca actactgccc cctatctgct acgacagttg
     5881 gcgttaacat cttcctggtt gaatacggtc cattaatttc ttgctatccc cattaagagg
     5941 gtcgtactcg atttttctgt catgatcatt aaggcaaaca acaaaatcat tcccacctgg
     6001 gttttatccg gtatgaaagg gttgaaggaa agaaccggac attccatcat ttttgaaaat
     6061 ctgatgacag gattgtttga gggattgcag attacacggc ttaagaaaga agccctgagc
     6121 tccttccgat agaagctgac gaagggctgg gagatctaac gcatcggaca ttgccagcac
     6181 aggaatttgg tactctaacc atcgtaattc atcgagcatg gttcgcccgt tcatcacggg
     6241 catatgcata tccaacaaaa ttccatcgac cctataattc atcaccacgt ccaggccttc
     6301 ccgtccattt cctgccacat acacgtgata ttccgaatct tcaaggacag tttttagtaa
     6361 ccctgccact gaaggatcat cttcaaccac caaaatatac ggctggtttt aggtgatatt
     6421 taaggtgcaa tgtgctgggt ctggtgtctc aagttctgga atttcccata catccgtcca
     6481 atgggaagag gaatgcaggt gcatcatgaa tgtcctttct ttccgattga tcatacctat
     6541 tgaaatcgag aattctatta gcgggtttta aaccttttca ctggaatatc tgtaacaaag
     6601 ccgttcagtg aatttgtccg gggggtcgct ggataacaac cgggaaagtg gatggctcac
     6661 ttctgctggc cgcatgaaat gtcctcaagt ttcttgatca gtaaaatcaa gtggagggga
     6721 aatacctata ccaaaaaagt ggggcgcgta actcaaggga agttccgccc cccctttctg
     6781 agagcctgaa tatggtgttg ttcatggaag agaagtctgc ggttgggcct ccacggcttc
     6841 tttttcagat tgatagatag gaacaatctc tgagaggtgg gtcgattcta agagatattt
     6901 gaccgtaggt tgcgggctga cgatgctgag gtgaacgtga ttgggcctca tcttgtgata
     6961 ccaaaggaac aattgtccaa gtcccatcga atcaatccag gtaatctcag caaaattcag
     7021 gatgatatgt tgacagccca tttcctttgc tccgaggatg gcgacttcaa ggccta
//
LOCUS       DAORAQ010000132         7019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1628_length_7019_cov_65.136703, whole genome shotgun sequence.
ACCESSION   DAORAQ010000132 DAORAQ010000000
VERSION     DAORAQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_7019_cov_65.136703"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKM72_10695"
     CDS             <1..464
                     /locus_tag="PKM72_10695"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNP61301.1"
                     /translation="WIICELEASLVLFFTGVSRSSEKIIADQRTNVEAGTVLALSAMH
                     AMKREACTMKESLLKGDFTGLVESMREGWDSKKRSAKSVSNAHLDEIYEAAIKAGALA
                     GKVSGAGGGGFMMFFVAPEKRMDVIFALKKFSGQVSNCHFTKYGMQGWKID"
     gene            537..1559
                     /locus_tag="PKM72_10700"
     CDS             537..1559
                     /locus_tag="PKM72_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNP61302.1"
                     /translation="MFSDRMRKLAYGSGLLGLYHRLRNQRTLTVVMFHRVLEISDPRW
                     HSCDPEYTLEVGLFECCLAFFRRHYNIVSVDDVIAARRGARALPPRALLITFDDGWSD
                     NVDFALPRLRAASMPGLLFVVADVIGRRLPFYQERLVGGWRLGRIIAGKLLAATGSAQ
                     LLRGNTLVDLRLAIAVLEKLDDNARERVLSAFADETDDGLSHMVASEDLRTLELGGIA
                     IGLHGKTHTPMTRASDLDAELGGARGIVAEHLESTKPPVTMSFPHGSYDPAIVQCACE
                     AGYELVFTSDPTLNLLESRPGWLLGRVGFEQSDIVDRHGAFRADKLALLLFCKPQRVL
                     ATDSTD"
     gene            1803..2525
                     /locus_tag="PKM72_10705"
     CDS             1803..2525
                     /locus_tag="PKM72_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNP61303.1"
                     /translation="MVQIGIRKAIVLAGGRGERLQSVVPDLPKPMAPVGGRPFLEYIL
                     DKIVDAGVTDVIISVGYKAEVIQEHFGRAYRSLQIRYSRESYPLGTGGAMALALKGED
                     SSPALVFNGDTLVDIDYSALMVWYEQTIAQVAIVLRQVPDVSRFGSVILLGERVVEFQ
                     EKGQQGPGLVNAGVYVVRPEIFSQYEFGSGEHFSFETDILQAYCRELQPRAFLTKGYF
                     IDIGTPGDYERAQKEAVFSRFN"
     gene            2558..3361
                     /locus_tag="PKM72_10710"
     CDS             2558..3361
                     /locus_tag="PKM72_10710"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNP61304.1"
                     /translation="MGYEKEFEQFWEKGYLIVPNLFDQEEMHILKNIIVNHDGMKQRT
                     EGLVQKMSQGKRPSFETIFVWNDTAGNDVFAKATRRASIFDRLGFFFNDSIYVYHNKI
                     ALKYPGMVGFRYHQDYAYWYGMGNLYPNMATVLIAIDPMTKENGCLKILEGSHKMGRI
                     DHVFHDGVSDSGVCQDRLAVIETRLPEVFVELKRGDVVMFHCNTLHGSNDNHSAHSRI
                     ALIGCYNTKANNPYKSAGGHPFYHPQERVLEKITEQDSKSLPDFDLQFS"
     gene            3484..3720
                     /locus_tag="PKM72_10715"
     CDS             3484..3720
                     /locus_tag="PKM72_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61305.1"
                     /translation="MRYLGSFLVVSIIVIVFGFEGLASAYLDPGTGSMVLQLLLGGIA
                     GAVVILKLYWRRFVGLFRGNAQEESEHPSSEDQK"
     gene            3720..5120
                     /locus_tag="PKM72_10720"
     CDS             3720..5120
                     /locus_tag="PKM72_10720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP61306.1"
                     /translation="MGGVQPLPGSFRDPSGNVYQVEGGIFRTVNECFSQDFDFVTSTG
                     LFKKLAAEDLLLPFEVVSSEVLNLSDPKPRYVLKAPRLPFISYPYEWSFPALKAAALL
                     HLRIHLQALEVGATLSDASAYNIQFQGNHPVFIDHLSFRRYQSGEMWVGHRQFCEQFL
                     NPLLLRALFGISHNAWFRGTQEGIGTGEIRRLLKGRHYWKWNVLTHVVLQDLFQKTST
                     KRTKSLQDHSLAKASLPLSSFKAMLGKMSHWISQLKPLNKGGSTWGDYDKTCSYSSME
                     TKSKKQFILEFAQKVQPSLLWDFGCNTGEYSETALNGGARNVIGFDFDQGALEGSYYR
                     AVERRLSFQALFMDAANPSPSQGWNGREREGLQSRASADAVLALALVHHLAIARNIPL
                     VQVIKWLITLAPQGVIEFVPKNDPMVQELLSLRQDIFPDYTAEHFMALLTKKAVIIKS
                     ETVSKSGRLLVWFKRM"
     gene            5320..>7019
                     /locus_tag="PKM72_10725"
     CDS             5320..>7019
                     /locus_tag="PKM72_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNP61307.1"
                     /translation="MTGKRIVLNDFLHLFALVGFAIAQPLYDLLGQNPEFFVSHKASS
                     GLIMGMIFVLSMCMPLGLVLLELAAWVVGERVRNSLHLIFVFALAFLIVLPPAQRLVD
                     GTDFLVVGFALFTGLFFSVLYVRWQATRLFLTVLSPVVVAFPLWFLMFTPAGRLVMPD
                     VTEAQNDIVITNPVPVVLVVLDEFNITALLDAKGLIDPVRFPNFAALASESQWFTNAA
                     ATYVETAVAVSAILTGRKPRADVRLNPTATDHPQNLFTLLGDHYRLNVIESLTRLCPH
                     ERCKEEEGADESFRDKSFFADLVVIFLHIISPKKLARHLPPLHAQWTGFGEKLLKKGI
                     FSQAKVGSHFDPSPGGNNSRETRLTSFLSQIKESSSPVLHFLHVVLPHTKYEYLVSGH
                     QYSSKGGHIPDGWIDGPGWGGRWIGKEPMILTAYHQYLQQIGYVDQFLGKLRSSLENA
                     HLYDKALIIVTADHGVSFQRGLSMREVQKGNESDILKVPMFVKLPGQHQGSINERLVS
                     GIDVLPTIADVLDFKIPRDIDGLSMLDNVTPPRDEIDFLGVGNIKAQDLKGFPRLNWQ
                     V"
BASE COUNT         1685 a         1521 c         1797 g         2016 t
ORIGIN      
        1 actggatcat ctgtgagctg gaggcctcat tagtgctttt ttttactggt gtgtcacgat
       61 cttccgaaaa gatcattgcc gatcaacgta caaatgtcga ggctggcacg gtcttggcac
      121 tttctgccat gcatgctatg aagcgtgaag cttgtacgat gaaggaaagt ttgctgaagg
      181 gcgatttcac tggattggtt gagtccatgc gtgagggatg ggacagcaag aagcgatcag
      241 ccaagagtgt gtccaacgca catctcgatg aaatctatga agcggctatc aaagcaggag
      301 ccttggccgg caaggtgtcc ggggctggcg ggggagggtt tatgatgttt tttgtcgcgc
      361 cggaaaaacg aatggatgta atttttgcgt tgaaaaaatt ctcgggccag gtcagtaact
      421 gtcattttac caaatacggg atgcaaggtt ggaagataga ttaaatcgaa tcgtgtagaa
      481 gaagtcagac cgtgtttgat ttcttctgaa ccgttgtctt gaaagaggtc ccttgcatgt
      541 tctctgatcg tatgcggaaa ctggcttatg gctccggcct gctcggcctc tatcatcgcc
      601 ttcgaaatca acgcacgctg accgtggtta tgttccatcg tgtgctagag atttccgatc
      661 cgcgatggca ttcctgcgat ccggaatata cgcttgaagt gggtttattc gaatgttgtc
      721 ttgctttctt caggcggcat tataacattg tatcggtcga tgacgtcatc gcggctcgca
      781 ggggtgctag agcattgccg ccacgcgcct tgctaattac ctttgacgat ggctggtcgg
      841 ataacgtcga tttcgccttg ccccgcttac gtgctgcatc tatgcctggc ctgctgttcg
      901 tggttgccga tgtgataggt cgtcggctgc ccttttacca ggaacgcctt gtgggtggct
      961 ggcgccttgg cagaatcatt gcgggaaaat tgctggctgc gacaggcagc gcacaactgt
     1021 tgcgtgggaa taccctcgtg gacttacgcc ttgccattgc ggtcctcgaa aaactggatg
     1081 ataacgcacg cgaacgggtc ctctcagcat ttgcagatga aactgatgac ggcctgagcc
     1141 acatggtggc tagcgaagac ctcaggacac tcgagttggg tggaatagca attggtctgc
     1201 acggcaagac ccatacgcca atgacccgcg ccagtgatct cgacgcggag cttggcgggg
     1261 cccgtgggat tgttgctgaa catcttgagt caaccaagcc acccgtgacg atgtcatttc
     1321 cgcatggaag ctacgatccg gccattgtgc agtgtgcatg cgaggccggg tatgagctgg
     1381 tcttcaccag cgacccgaca ctcaatttac tggagtcgcg gccggggtgg ttgcttggcc
     1441 gggtcggctt tgagcagtcg gacatcgtcg atcgccatgg ggcattccgt gccgacaaac
     1501 ttgcccttct gctgttctgc aagccccaac gagtactggc tacggacagc acggactagg
     1561 caatagtact attcagcttt gtgcgctgag gttgggtgcc aattgctgaa gtggataggt
     1621 gtcccttcat ctgctattgg gggtaaatgg ttgatcatat taggtcaatt ttctacaggc
     1681 agagatgtta attttttggt gttgttgcct tatgcgtcat cagctgaatt gttcgtaaaa
     1741 aatccccttg tgtcgtcatt ggctggtgtt tggtttcacg tgtgtagaca ggagcgtttc
     1801 atttggttca aataggaatc agaaaagcca ttgttctggc tgggggacgt ggtgaaagac
     1861 ttcagtctgt cgttccggat ctgcctaaac ccatggcacc tgttggaggt cggccatttt
     1921 tggaatatat tctcgataaa attgtcgatg ctggtgtgac cgatgtcatt atatcggttg
     1981 gatacaaggc cgaggtcatt caagagcatt ttggtcgcgc ttaccgctca cttcaaatca
     2041 gatattcaag agaaagctat cctttaggaa ccggaggagc gatggcctta gctctaaaag
     2101 gggaagattc ttctccggca ttggtcttca atggggatac gcttgttgac attgattatt
     2161 ccgcactgat ggtctggtat gaacagacca tagctcaggt tgcaatagtc cttcggcaag
     2221 ttcctgatgt gagccggttt ggttccgtca tacttttggg agaacgagtc gttgaatttc
     2281 aagaaaaagg tcaacagggt ccagggctgg tgaacgcagg tgtgtatgtt gttcgaccgg
     2341 aaatattttc ccaatatgaa tttggaagtg gagagcactt ttcctttgag acagatatcc
     2401 ttcaagcata ttgccgagag ctgcaacctc gcgcattcct caccaaagga tactttattg
     2461 atatcggcac gccaggcgat tatgaacgcg ctcagaaaga agcggtcttc agtcggttta
     2521 actgagcggc atcggttccg caacaatgga gagtgagatg ggatacgaga aagagttcga
     2581 acagttttgg gaaaagggtt acctcattgt tccaaacctg tttgatcagg aagaaatgca
     2641 tattctgaaa aatatcatcg tgaatcatga tggtatgaag cagcggaccg aaggtcttgt
     2701 gcaaaaaatg tctcaaggaa agcgtccttc atttgaaaca atttttgtgt ggaatgatac
     2761 agccggaaat gacgtctttg ccaaagccac tcgtcgtgcg tcgattttcg atcgattggg
     2821 atttttcttc aacgattcta tctatgtgta tcataataaa attgccttaa aatatcctgg
     2881 tatggtcgga tttcgctatc atcaggatta tgcgtattgg tatggaatgg gaaatttgta
     2941 tccgaatatg gctacggtac tcattgccat tgatccgatg accaaggaaa atgggtgttt
     3001 gaagattctc gaggggtccc acaagatggg taggattgac catgtgtttc atgatggagt
     3061 gtccgacagc ggtgtgtgtc aggaccgact cgccgtgatt gaaacgcgtt tgccagaagt
     3121 gtttgttgaa ttgaaacggg gagatgtggt catgtttcat tgtaataccc tgcatggttc
     3181 taatgataac cattcggccc attcccgaat cgccttaatc ggttgttata acaccaaagc
     3241 aaataatccg tataagtcgg cggggggcca tcccttttat catccccaag aacgagtatt
     3301 ggagaaaatt accgaacaag attcaaaaag ccttcctgat tttgatttgc aattttcctg
     3361 attttacttc agagttggta ccgaaggatg actgacccgt taccgattga cgataatgga
     3421 tgtacaaata aggatcgaat ctgaaatgaa cagggagtta atagtccagg gaggtaaaag
     3481 caaatgagat atttagggag ttttcttgta gtcagtatta ttgtcattgt gttcggattc
     3541 gaaggtttgg catcagctta tcttgatcct ggtaccggaa gcatggtgtt acagctcttg
     3601 ttgggtggta tcgccggtgc cgtggtgatt ctcaaattgt attggaggcg atttgtcgga
     3661 ctatttcgtg gaaatgctca agaagaatct gaacatccct cttccgaaga tcaaaagtaa
     3721 tgggtggcgt tcaacccctt ccaggatctt tccgggatcc cagtgggaac gtgtatcagg
     3781 ttgagggggg tatctttagg acggtcaatg aatgcttttc tcaggatttt gattttgtca
     3841 cttcaacagg actattcaag aaacttgcag ccgaagactt gctccttcct tttgaggtcg
     3901 tatcttcaga agttctgaat ctctcagatc ctaagcctcg gtatgtgttg aaagctccca
     3961 ggctcccatt tatctcctat ccgtatgaat ggtcatttcc ggctttgaag gccgctgccc
     4021 tccttcattt acgcatccat ttgcaggcat tggaagtggg tgccacgctc tccgatgcct
     4081 cggcttacaa tattcaattt cagggaaatc accctgtttt tattgatcac ctctctttta
     4141 ggcgctacca atccggtgaa atgtgggttg gtcatcgtca attttgcgaa caattcctga
     4201 atcctctgtt acttcgtgca ttgttcggaa tatcccacaa cgcctggttt cgagggacgc
     4261 aggaaggaat tggaactgga gaaatccgaa gactactgaa ggggcgacac tactggaagt
     4321 ggaatgtatt gacccacgtc gtcttgcaag atcttttcca aaaaacctcc accaaacgta
     4381 ctaaaagctt gcaggatcat tcgttagcca aggcctcgct ccctctttct tctttcaagg
     4441 cgatgctggg aaaaatgtct cattggatta gccaattgaa acctctgaat aagggaggaa
     4501 gtacctgggg agactacgat aagacctgta gttattcttc aatggaaact aaatctaaaa
     4561 aacaattcat tcttgaattt gcccaaaagg tccagccctc actgttatgg gattttggat
     4621 gcaataccgg ggaatattcg gaaacggcat tgaatggcgg ggccaggaac gtaatagggt
     4681 ttgactttga tcagggggcc ttggagggta gttattatcg agccgtcgag aggcgtctct
     4741 cctttcaggc tctctttatg gatgcggcga atccgagtcc gagccaaggg tggaatggtc
     4801 gcgagcgaga aggtctgcag tccagggcct cagcggatgc cgtattagca ctggctcttg
     4861 ttcaccatct ggctattgcc aggaatatcc ctttggtcca ggtcataaaa tggctgatca
     4921 cgcttgcgcc acagggagtc attgaatttg ttccgaaaaa tgatcctatg gtgcaagagt
     4981 tattaagcct gcgtcaggat attttccctg attacaccgc ggaacatttt atggcgcttc
     5041 ttacaaaaaa ggcagtgatc attaagagcg aaaccgtctc aaaaagcgga cggctgttgg
     5101 tgtggtttaa acgaatgtaa ttcagaaatc taatacctgg tcaaatgtac gtgaaaattt
     5161 gaaccatcat gaaaaatccg aggtgtcgct gaaaaacgcc ttgaactttt ctgcacgctt
     5221 tcctcaaaaa cacttttcat cctcccccta aaatctgtat ttgagcttta cctttttctc
     5281 tagggaccta aatgaccact tcgtcaggtg agccaaaaaa tgactggtaa gcgtattgtc
     5341 ttgaatgatt ttttgcattt gtttgctctc gttggctttg ccattgccca gcccctttat
     5401 gatcttcttg gacaaaaccc cgagtttttt gtctctcata aagccagttc tgggcttatc
     5461 atgggtatga tctttgtcct atcaatgtgt atgcctttag gtctggtgct gcttgagctc
     5521 gccgcttggg tggttggtga acgtgttcga aatagcctgc atctcatatt tgtctttgcc
     5581 ctggcgtttc tcattgtcct gcctccggct cagcgattgg tcgatgggac cgatttcttg
     5641 gttgtcgggt ttgcattgtt caccggatta ttcttttcgg tcctttatgt tcgctggcaa
     5701 gcgacccgtc tattccttac ggtgctctca cccgtggtgg tagcatttcc gctttggttt
     5761 ttaatgttta ctcccgccgg tcgtcttgtt atgcctgatg tcactgaagc acagaacgat
     5821 attgtgatta ctaacccggt cccggttgtc ctggtggtgc tggatgaatt caacattaca
     5881 gcattattag atgccaaagg tctgattgat cctgtgcgat ttcctaactt tgcggccttg
     5941 gcttcggaaa gccagtggtt taccaacgcc gctgcaacgt atgtagaaac cgcagttgcg
     6001 gtgtctgcaa ttttgaccgg gcggaaaccc agagccgatg ttcgactcaa cccgacggca
     6061 acagatcatc ctcaaaatct gttcacacta ttgggggacc attatcgtct taatgttatt
     6121 gagtcgctga caagattatg tcctcatgaa cgttgcaagg aagaagaggg tgcggatgaa
     6181 tcctttcgtg ataagtcgtt ttttgcagat ctggtggtga tatttttaca tatcatttct
     6241 cccaaaaaat tagcacgaca cttaccaccg cttcatgctc aatggacggg tttcggagag
     6301 aaattgttga aaaaagggat tttttctcag gctaaggttg gtagccattt cgatccttct
     6361 cctggcggaa ataactccag agagacgcgc cttacctctt tcctttccca gataaaagaa
     6421 agctccagtc ctgttctcca ttttcttcat gtggttcttc ctcatacgaa atatgagtat
     6481 ctggtgtcgg ggcatcaata ttcctccaaa ggaggacaca ttcctgacgg ttggattgat
     6541 ggacctggtt ggggggggag atggattgga aaagagccga tgattctcac cgcgtaccac
     6601 caatatctcc agcaaattgg atatgttgac cagtttttag ggaaattgcg cagttcactt
     6661 gaaaatgcac atctttatga taaggctctt ataattgtca cagccgatca tggtgtctcc
     6721 tttcagcgtg ggctatccat gcgagaagtg caaaaaggaa atgaaagcga tattctaaaa
     6781 gttcccatgt tcgtcaaatt gcctgggcaa catcaaggga gcatcaatga gcgactcgtc
     6841 agcgggattg atgtgttacc aactattgct gatgtccttg acttcaagat cccccgggat
     6901 atagatgggc tttctatgtt ggataatgta accccgccaa gggatgagat agattttctc
     6961 ggagttggaa atatcaaagc ccaggacctg aagggatttc ctcgtctcaa ttggcaggt
//
LOCUS       DAORAQ010000133         7003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1632_length_7003_cov_66.657311, whole genome shotgun sequence.
ACCESSION   DAORAQ010000133 DAORAQ010000000
VERSION     DAORAQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_7003_cov_66.657311"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..575)
                     /locus_tag="PKM72_10730"
     CDS             complement(222..575)
                     /locus_tag="PKM72_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61308.1"
                     /translation="MLNQGEKLLIVHRRLFEKDTPRFFVGEVLIYEAGIVKVKGYTFV
                     KDMFSGNMQKKSDLRTKVMSIVSGTLIVYQLPVTALLDSMGFSLDQDGGLVLTDEGGF
                     SMDVSESLYKHETHQ"
     gene            complement(656..1273)
                     /locus_tag="PKM72_10735"
     CDS             complement(656..1273)
                     /locus_tag="PKM72_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP61309.1"
                     /translation="MRAPYVPGSFRQRNVEKILKASATGQVKVGQDNLLSVSQKNAGP
                     TSLQARLYKQVESFTQSSRAKICARDIMSSPVVTLPVMSSLSQAWDVVHSTRYRHIPI
                     IGVDNAVIGILSDRDLFRGTVKSALSRTERAVKEAEGSIQHLVSHPVLVASPEAELRA
                     IARVLLEERIGALPIVSEAGGLMGMITRSDILRALVAHSDFDQWV"
     gene            complement(1411..2727)
                     /locus_tag="PKM72_10740"
     CDS             complement(1411..2727)
                     /locus_tag="PKM72_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNP61310.1"
                     /translation="MNTSKTGRSQGKSMKLGGSWLGRVMAFVLVGSLMSTGAYGEHLD
                     VSGGLTDTSSDCHGVFLGKQIPGETLTRILAAHARWLADHQDPKGVQANLCGADLSEV
                     DFREEDLSGANFQGANLTRAVFTTARLIHVNFQNANLTEASFYSVDMRGANVSGASAR
                     LADFRKADLTQTNFTGADLREANLAESAFRLGVLRGADLEEAYLVRANFEMANLAEAS
                     LIRVDGREANFRRALLVNAKLLEADFNRSDFQEADLRSADISGADFGSANLSEAVLNH
                     ANLREVNLDRAVLFQANLEDANCAGASFQNADLLEANLMNSNLIRATLDEAYLAKANL
                     LGANLEGADFSWANLAEADLSQSILEFAVMFRTNLHEAKLDGARFYGADLRGANVEKA
                     SFQGTRLEGVAIRYAQGLNQTQLNQACVDARTRLPEGLVGPSPCLR"
     gene            complement(2819..3727)
                     /locus_tag="PKM72_10745"
     CDS             complement(2819..3727)
                     /locus_tag="PKM72_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61311.1"
                     /translation="MKILCAVDGSEFSLWALECVGKLFRHSVKELILLHAVDPRILRG
                     GGGKRPKMDEPGTKDISQKLEAAGQKVLTDCAHRMEMVLSQATTKPFAAIKTALVKGH
                     AADSIISYAERSLPDLVVVGSRGMNDLPGYLLGSISRTVLTHGPCSVLTVKSPLDVPA
                     SVLLALDGSKPSKFAANRVKEWLSPEEVTLHLVSVVPDILTNASKKMFPKSRLKSLVA
                     PLRQHAQECLEEYRKLFLKEGFQVVGERLQGNPREKIVESVPKCHTQLVVMGSKGLTG
                     VERFTMGSVSEWVSAYAPSSVLVVRH"
     gene            4241..5719
                     /gene="atpD"
                     /locus_tag="PKM72_10750"
     CDS             4241..5719
                     /gene="atpD"
                     /locus_tag="PKM72_10750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNP61312.1"
                     /translation="MAPDLNHSNLGAVVSVRGSVVDIRFEDHLPPIYSLLRTGQDGEI
                     AIEVLAQLDIHRVRGIALTPTQGLARGMVVEDTGGPLMAPVGKGILSRMFDVFGNSID
                     REPAPTKIQYRSVHRAPPSLARRSTKSEIFETGIKIIDVLLPLERGGKAGLFGGAGVG
                     KTVLLTEMIHNMIGHHQGVSIFCGIGERCREGEELYREMKEAGVLPNMVMVFGQMNEP
                     PGSRFRVGHAALTMAEYFRDDEHRDVLLLIDNIFRFIQAGSEVSGLMGQMPSRLGYQP
                     TMGTELSRLEERIANTDTGAITSIQAVYVPADDFTDPAAVHTFSHLSASIVLSRKRAS
                     EGLYPAIDPLQSNSKMATPGTIDERHYRIAQEVRRTLAQYAELKDIIAMLGLEQLSLE
                     DRKVVARARRLERFLTQPFFTTEQFSGLKGQLVSRKDALDGCERILRDEFKDYPEHAL
                     YMIGAIDQARETAKSGNPAAQGEQKSNNTSTGDPNSNGKSER"
     gene            5757..6161
                     /locus_tag="PKM72_10755"
     CDS             5757..6161
                     /locus_tag="PKM72_10755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNP61313.1"
                     /translation="MQPALMTLKILLPFRIFAEQNAVTRIVAETRQGSFGILPQRLDC
                     VAAFVPGILIYETDGEDEKYVALDEGVLVKSGPNVLVSVRNAIAGTDLHQLREAVERE
                     FLTLDEQEQSLRSVMAKLESSFVRRFADFRHD"
     gene            6154..6492
                     /locus_tag="PKM72_10760"
     CDS             6154..6492
                     /locus_tag="PKM72_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNP61314.1"
                     /translation="MTDDPEKKPLGEETTFTRQVGIKAARKLKAQRHTTRTIWFGLGM
                     MGLIGWSVAIPTLLGASLGLWLDKHYPVPHSWTLTLLFIGLIIGCVNAWHWVTREDQE
                     IRAEQENHDE"
     gene            6485..6796
                     /locus_tag="PKM72_10765"
     CDS             6485..6796
                     /locus_tag="PKM72_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNP61315.1"
                     /translation="MNEIFMIMPALITGMLLGAMFFGGLWWTIRKGLSSTQPAFWFIG
                     SLLVRTSITLAGFYMVSNGQWERLLVCLAGFTIARPIVMRFTGLADNPNRPAQEPSHA
                     P"
     gene            6786..>7003
                     /locus_tag="PKM72_10770"
     CDS             6786..>7003
                     /locus_tag="PKM72_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNP61316.1"
                     /translation="MRLSPDDIIFWQYGFIKLNATIAFTWGLMLLLAIGSTVITLHLS
                     TGLTRSRWQNFLEIIVTAIENQIEEVGL"
BASE COUNT         1690 a         1952 c         1735 g         1626 t
ORIGIN      
        1 tccgtagtac ctttgccacg gtcgctaatc caacctgaat attcacgata tgttcctccc
       61 gctgactctt ttcgaaatgg ccattctctc gctaaaggcc agaaactcgc acccatgcaa
      121 atccttgcat tgaccagggg ttggccatag gagactgaac gcttagacaa aaaaagaagg
      181 aaagtggcaa gccttgggaa agaataaaca aggacgtgtt gttattgatg cgtttcatgt
      241 ttatagaggg attccgacac atccatcgaa aatccacctt catccgtgag caccaaccct
      301 ccatcctgat ccagactaaa tcccatcgaa tccaagagag ccgtcaccgg caattgatac
      361 acaataaggg tccctgagac aatcgacatg acctttgtgc gtagatcgga cttcttttgc
      421 atgtttccgc taaacatatc tttgacaaac gtataccctt tcaccttgac gatcccggct
      481 tcatagatca gcacttctcc cacaaaaaac cggggcgtat ctttttcaaa tagtcggcga
      541 tggacgatta acagtttctc tccttggttt agcatgggtt tcctccttta aatcggcaaa
      601 aagatacgag aggaattgtc cgcttcctca ccgtagggcc gagaatctct ttcgcttata
      661 cccattgatc aaagtcagaa tgtgcgacca gagcacgcag gatatcactt ctggtaatca
      721 tacccattaa gccccccgct tccgacacaa tcggcaaagc accaatcctt tcctccaata
      781 acaccctcgc aatggcgcga agctcggcct ccggggacgc aaccaagacc gggtgactga
      841 ccagatgttg gatagaaccc tccgcctcct taacggccct ctctgtccgt gaaagagccg
      901 acttcactgt tccccggaac agatcacgat cagacaatat tccaatcacg gcattatcca
      961 ctccgatgat agggatatgt cgatatcgcg tggaatgcac aacatcccag gcttgggaaa
     1021 gcgaggacat gacaggaagc gtgaccaccg gggaggacat gatatccctg gcacaaattt
     1081 ttgcgcgaga ggattgcgtg aacgattcaa cctgtttata caggcgggcc tgcaaagaag
     1141 ttggtccggc atttttctga gaaaccgaaa gaagattgtc ctgcccaacc ttcacctggc
     1201 ctgtggcaga ggccttcaag attttctcaa catttctctg ccggaacgaa cccggcacat
     1261 aaggggcccg catgcctcct ggctcaaata cgatgaacat tccggcactc cggggcttgt
     1321 tgtgtggatt caaggtacat tcgtatcaat aagatgttgg catgttcgtt gaaaccccta
     1381 aacccttttg cgaggtcgca ctgttatcgc tcagcgcagg catggagatg gtcccaccaa
     1441 tccttccggc aagcgggttc gtgcatcgac acaggcctga ttgagttgtg tctggtttaa
     1501 gccttgggca taccggatgg ctaccccctc caaacgcgtg ccctgaaaag aggctttctc
     1561 gacattcgct cctcgaagat ccgccccgta aaaccttgct ccatcaagtt tggcctcgtg
     1621 tagattggtt cggaacatca cggcaaactc tagaatgctc tgtgaaagat cggcttcagc
     1681 caggttcgcc catgagaagt ccgccccttc caggtttgcc cccagcaaat tcgctttggc
     1741 gagataggct tcgtccaatg tcgcccggat gaggttgctg ttcatcaaat tagcctcaag
     1801 caaatcggca ttctgaaaac tcgcgccggc acaattcgcg tcttcgagat tcgcttgaaa
     1861 cagcacggcc ctgtccaaat tcacttcacg gagattggca tggttcagta cggcctcact
     1921 cagattcgct gatccaaaat cggccccgga aatatcagcc gaacgaagat ctgcttcttg
     1981 gaagtcgctt cgattgaaat ccgcttcgag tagtttggca ttgacgagta aggcccggcg
     2041 aaaatttgcc tctcgaccat ccactcgaat gagggaagct tccgcgagat tggccatttc
     2101 aaaattcgct ctcaccagat aggcttcctc tagatctgct cctcgcaaga cgcctaggcg
     2161 gaaagcagac tcggcaagat tcgcttctcg caaatcagct cccgtgaaat tggtctgggt
     2221 caaatcggct tttcgaaaat ccgccaaccg ggcactggca cctgagacgt tggctccgcg
     2281 catatcaacc gaatagaaag aggcttcggt gaggttcgcg ttttgaaagt tcacatggat
     2341 taacctagcc gtagtgaaca cggcacgagt caggttggcc ccctgaaagt tggcaccgct
     2401 caaatcttcc tcccgaaaat cgacttcgga caaatcggcc ccgcataaat tggcctgcac
     2461 ccctttcggg tcttgatgat cggcgagcca tcgggcatgt gcggctaata tccgggtcag
     2521 tgtttctccc ggaatttgtt ttccgaggaa taccccatga cagtccgagg aggtgtctgt
     2581 gagaccaccg gatacatcca gatgctcccc ataagctccc gtggacatca gactcccgac
     2641 caatacgaat gccatcaccc ttccaagcca tgacccgccc agcttcatag actttccctg
     2701 cgatcgtcct gtcttacttg tgttcaacaa ttcccctcct ttaagtcgtg tagtgcccga
     2761 tcttcatata aagattacgc ataacagcag gcaaagcctt cgccttttac aatgtgttct
     2821 aatgccgaac gaccagcacg gaggagggcg cataggcgct cacccattcc gaaacacttc
     2881 ccatggtaaa ccgttcaaca ccagtgagcc ccttggatcc catgaccacc aattgcgtat
     2941 gacattttgg aaccgattcg acgattttct cccgtggatt cccctgaaga cgttctccca
     3001 ccacttgaaa cccttccttg agaaacaatt ttcgatactc ctcaaggcat tcctgagcat
     3061 gctgtcgaag gggagccacc agggatttga gtcgtgattt cggaaacatc ttcttggacg
     3121 cattcgtcag aatatcgggc acaaccgaca ccaggtgaag ggtcacctct tcaggagaca
     3181 gccattcttt cacccggttc gccgcaaact ttgatggctt ggacccatcc agggcgagta
     3241 agacggaagc cggcacgtcg agtggactct tgaccgtcaa gaccgagcag gggccgtggg
     3301 tcaaaaccgt tctcgaaata ctcccaagta aatatcccgg gagatcgttc attccgcgtg
     3361 atcccacaac caccaaatca gggaggcttc gctcggcata ggaaataatg gaatcagccg
     3421 catggccttt caccaaggcc gtcttaatcg ccgcaaacgg cttggtggta gcttggctta
     3481 acaccatttc cattcgatgg gcacaatcag tcaagacctt ctgacccgct gcctccagct
     3541 tttgggaaat atctttggtg cctggctcat ccattttagg tcgctttccc ccaccacctc
     3601 tcaatatccg tggatcaacc gcatgcaaca ggatcaattc ttttaccgaa tggcgaaaca
     3661 atttccccac acattcaaga gcccagagag aaaactccga cccatcaacg gcacacagaa
     3721 ttttcattgg tacatctctt caatcccatt tacacagaac acatcacaca attccattgt
     3781 tcatgacgac atacacttca ctcgcaggaa tcctgccaag ttgaatattt ttcaacaaaa
     3841 atttttttat tcagaatttg gaattttcac gaaaagaaaa cgtagagccc gttcactcca
     3901 tccccacatg cccttttttg ggactattct ccttcatcac actgtagcct ttttcctctt
     3961 tttccccgag aacaattgat cacgggccaa cacattatct ggtccgagtg tcactcaaac
     4021 ctggcactca gggaaggaac atggtctggc attttctttg ctgagttctc ttatgaatat
     4081 tatgatcatc agtccttttc tcactgattg ttagcgttgc cggaatccgt atcccaaatt
     4141 tttcaggacg gcagatttgg tggtttggac tgtaaagtgg cgcatgtcat gagtctcaac
     4201 ctcctgattc aaagccatat accatcccag gaatctttac atggctcctg acctcaatca
     4261 ctcgaatctc ggcgcggttg tctcggttcg cggcagcgtg gtggatatcc ggttcgagga
     4321 ccatttaccg cccatttact cgttgttgcg cacaggacag gatggagaaa tcgccattga
     4381 ggtgttagcc caactcgaca tacatcgcgt gcgcggaatt gcactgacgc ccacacaagg
     4441 cctcgcccgc ggcatggtcg tggaagacac cggcggaccg ctgatggccc cggtcgggaa
     4501 aggcattctc tcccgcatgt tcgacgtatt tggaaattcc atcgaccggg aaccggcacc
     4561 aacaaagatt caatatcgct ccgtccatcg agccccgccg tcgttagccc ggcgttccac
     4621 caaatccgag atcttcgaga caggcatcaa aatcattgat gtgctgttgc ctctggagcg
     4681 cggaggcaag gccggtctgt ttggtggagc aggcgtgggc aagaccgtcc tgctcaccga
     4741 aatgattcac aatatgatcg ggcatcacca gggggtcagc attttttgcg ggatcggcga
     4801 acgctgtcgt gaaggagaag aactctaccg ggaaatgaag gaagccggtg tactaccgaa
     4861 catggtcatg gtgttcgggc agatgaatga accgccgggc agccgcttcc gtgtggggca
     4921 cgcggcgctg accatggcag agtatttccg tgacgacgag catcgggatg tgcttctgct
     4981 catcgataac atctttcgtt tcatccaggc cggttcggag gtttcagggc tgatggggca
     5041 gatgccatca cgcctgggct atcagccgac gatggggacc gaattgtccc ggttggagga
     5101 acgcatcgcc aacaccgata ccggggccat cacgtcgatt caagccgtgt atgtgccggc
     5161 agacgatttc accgatccgg ccgccgtgca caccttctcg catctttccg cctccatcgt
     5221 gctgtcccgc aaacgggcca gtgagggact ctacccggcc atcgatccct tgcagtcgaa
     5281 ttcaaaaatg gccacgccgg gcacgattga tgaacggcat taccgcatcg cgcaggaagt
     5341 caggcggaca ctcgcgcaat atgcagaact caaggacatc atcgcgatgc ttggcttgga
     5401 acaactgtcc ctggaggacc ggaaggttgt cgctcgcgcc cgacggcttg agcggtttct
     5461 cactcagccg tttttcacga cggaacagtt ttcaggcctc aaaggacaac tcgtcagccg
     5521 caaggacgcc ctggacgggt gtgaacgcat cctacgggac gagttcaagg attatccgga
     5581 gcatgcccta tacatgattg gagccattga ccaagcacgg gaaacagcaa aatcgggtaa
     5641 ccccgcagct caaggcgaac agaagtcgaa caacacatcc acgggcgacc cgaactccaa
     5701 cgggaagtcc gaacggtaat cgaagccatc gtcgaaattg caaccaaagg tcaaccatgc
     5761 aaccggcgct catgaccttg aaaattcttc taccgttcag gatcttcgcc gaacagaacg
     5821 cggtgacgcg tattgtcgca gagacccgtc agggatcgtt tggcattttg ccacaacggc
     5881 ttgactgcgt ggctgcgttc gtgccgggaa ttctgatcta cgaaacagac ggagaggatg
     5941 agaaatatgt ggccctcgat gaaggggtgc tggtcaaaag cggccccaat gttctggttt
     6001 ccgtgcgcaa cgccattgcc ggaaccgatc tccatcaatt gcgcgaggct gtggaacgtg
     6061 aatttttgac cctggacgaa caggagcaga gcctccgctc ggtgatggcc aagcttgaaa
     6121 gtagctttgt gcgccgcttc gcggattttc gccatgactg atgacccgga aaagaagcct
     6181 ctcggggaag agacgacttt tacacgacag gtcggcatca aggctgcgcg caaactgaag
     6241 gcgcaacgac acacgacgcg gaccatctgg tttgggttgg ggatgatggg gctgatcggt
     6301 tggtcggtag cgattccaac gctactgggt gcatcgctgg gtctctggct ggacaaacat
     6361 tatcccgtac cccattcatg gacgctcacc ctgctcttca ttggcctgat catcggctgt
     6421 gtgaatgcgt ggcattgggt gaccagggaa gaccaggaaa tacgagccga acaggagaat
     6481 cacgatgaat gaaatcttta tgatcatgcc tgcgctgata acgggaatgt tgctcggagc
     6541 gatgtttttt ggtggcctat ggtggacgat tcggaaaggc ttgtcatcca cacaaccggc
     6601 cttttggttc atcggcagcc tgctggtgcg aacgagcata accttggccg gtttctatat
     6661 ggtctcgaac ggccaatggg agaggctgct ggtgtgcctc gctggattta ccattgcccg
     6721 tccgatcgtc atgcgattta ccggcctagc ggataaccca aacaggccgg cacaggagcc
     6781 cagccatgcg ccttagcccg gacgacatca ttttctggca atacgggttc atcaagttga
     6841 atgccaccat tgcgttcacg tggggattga tgctcctgtt ggctattgga tcaacagtca
     6901 ttacgctgca cctgtccacc ggcctgacac ggtcgcgttg gcagaatttt ctggaaatca
     6961 tcgtcacggc catcgagaac caaattgaag aggtgggcct gag
//
LOCUS       DAORAQ010000134         6986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1633_length_6986_cov_64.913000, whole genome shotgun sequence.
ACCESSION   DAORAQ010000134 DAORAQ010000000
VERSION     DAORAQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1633_length_6986_cov_64.913000"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2341)
                     /gene="recQ"
                     /locus_tag="PKM72_10775"
     CDS             complement(<1..2341)
                     /gene="recQ"
                     /locus_tag="PKM72_10775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNP61317.1"
                     /translation="MKNNLFPQQTGSPPLHTSTQPPLLSLLKEHFGFTAFRPLQEEII
                     RDVLAERDVVALLPTGGGKSLCFQLPALARSGLTIVVSPLIALMKDQVDGLRANGIAA
                     TYLNSTLTAQESQARLRGLYQGDYRLLYMAPERLMLPGYLNLIRNFQVNLLAIDEAHC
                     ISEWGHDFRPEYRRLVELRDQVPALPIIALTATATARVQKDIVTLLRLRNPACYVASF
                     NRPNLTYQVQPKDNPFGQVLAFLRTRPKDCGILYCQSRKTTESVAERLQDHGINAQPY
                     HAGLSAEERTRHQELFLRDEIQVICATIAFGMGINKPNVRFVIHYDLPKNLESYYQET
                     GRAGRDGLPSECVLLFSAGDTVKQQRFINEKPNFQEQQIANRQLQEMVQYAECGTCRR
                     RTLLQYFGEHVESENCGGCDNCLTPRETFDGTNAAQTVLTCVEEIRGSGGFSVGLNHV
                     IAVLTGANTEKIRRWGHDRLTSYGKGEAHRRPEWAAISRELIRLGYLQQTADPFTVLE
                     VTDQGQHFLQDPKTLMLTKPMSTPERKTPSPQDLGHDEMLFTRLRQLRKTLADEADVP
                     AYVIFSDVALRQMARDCPTNEQEFLQISGVGKRKLEEFGPKFLAVIAAHLENYAPLNN
                     PNESNHSPAPSLRDQNPASNQKGFGHQHAVEGYEKTSGSQIPTISPAQSQGRQDALSH
                     GQGRRGIETPSAHQIPVHDQAPRTRLADFSNRPARRKPLGDTVHQTLRRFGSGNSVEQ
                     IARERGLVTSTIYGHLEQAIQAGEPVDSTQLWTPEQELEM"
     gene            2707..3042
                     /locus_tag="PKM72_10780"
     CDS             2707..3042
                     /locus_tag="PKM72_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP61318.1"
                     /translation="MYVAERMQDTTLVLMISGRVTVYSRKVFQGMVKAARFSGAKHII
                     FNMQGVTFMDSIALGGLVLAYLDLNENHMAMSVVEPQHPVKTLFEDANFPDLIPTYPT
                     EETALQAIQ"
     gene            3524..5308
                     /locus_tag="PKM72_10785"
     CDS             3524..5308
                     /locus_tag="PKM72_10785"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNP61319.1"
                     /translation="MAYEHLLALRAELLREQLTGFVVPHTDEYQNEYLPPCAERLAWL
                     TGFTGSAGTAIVLRDEAAMFVDGRYTLQVADQVDEKSFSLHNSGDTSPHEWLALRVTP
                     TDRIGYDPWLHTPQDLERFQTATERVGAHLVPCVSNPIDAVWHDRPQQPSGVIKPHLL
                     EFSGQSSQAKREIFGRRFSEDGIDAAIITAPDSIAWLLNIRGSDVPHTPLPLCRAILY
                     TTGRVALFVDPKKITPGLQAHLGPDISLALPEEFEAALQQLGTHGNRVLCDPAKTNSW
                     IFTTLTQSGAVLLQKDDPCVLPKACKNPVEIAGAYAAHQRDGVAMCQFLAWLAREGSK
                     GQVTELEAVDYLDACRRKQAMWEDCSFPTISGAGSNGAIVHYHSTRETNRCLEPGTLY
                     LVDSGGQYLDGTTDITRTLAIGRPSDEHRDRYTRVLQGHIALATARFPEGTTGSQLDV
                     LARAPLWAMGLDYDHGTGHGVGSFLGVHEGPQRIGKAANAVALKPGMIVSNEPGYYKT
                     GAYGIRLENLVTVVKDESSQNSTRSFLGFDTLTIVPFERTLIATEVLSLKERQWVDTY
                     HARVWADLQSLVDTDTRAWLEQATQPLN"
     gene            5364..5675
                     /locus_tag="PKM72_10790"
     CDS             5364..5675
                     /locus_tag="PKM72_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61320.1"
                     /translation="MTPQKTNEEITEAIKTQMGKNPEITNVIVKGHLLQLHVTQGLFH
                     RLSADRERGRKIVLVLMEQMKQLTGLTDVAVWVYSENEKVIEGTVKAFGGDNVNFLFD
                     L"
     gene            complement(5711..5914)
                     /locus_tag="PKM72_10795"
     CDS             complement(5711..5914)
                     /locus_tag="PKM72_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61321.1"
                     /translation="MPSESKRPVRRSPVWMWISADGGRWRINHTPNGVWSCQIGLGGK
                     RVGSWKTGLPPSLDSWPKGTYQE"
     gene            6041..>6986
                     /locus_tag="PKM72_10800"
     CDS             6041..>6986
                     /locus_tag="PKM72_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNP61322.1"
                     /translation="MKNSDYPSSAIVPELSTGLVGQYEADVLCLSVYGKTVILIGTAH
                     VSQESVDLVKLVIEQEHPDRVCVELDTKRFEAISHPNRWESLDLKEIIRKQQLSTLMV
                     NLILSSFQKRLGDKLGVIPGTEMLEAIRMADKHGIPVTLADRDVRVTMRRAWRNTPMW
                     RKSLLMSSLMLSIFDTTEVSEEEIRNLKKQDVLSEMMKDLGKEVPTLKTVLIDERDHY
                     LAKKIVDAPGTRIVAVVGAGHVEGICRTLNERETVVLEELESIPPVSPVWKAVGWSIP
                     VLIVGSIAWIGWEKGLSAAGDNLLFWILANGIPSGIGGL"
BASE COUNT         1546 a         1686 c         1995 g         1759 t
ORIGIN      
        1 tcatttctaa ttcctgctcc ggtgtccata gttgtgtgga gtctaccggc tcacccgcct
       61 ggatggcttg ttccaggtgc ccatagatgg tgctggtgac caacccgcgt tctctggcga
      121 tttgttcgac cgaatttccc gatccaaaac ggcgcaaggt ttgatggacg gtatctccaa
      181 gcggctttct tctggctggc ctgttggaaa agtcagccag ccgagttctc ggggcttggt
      241 cgtgcacggg tatttggtgt gcgctcgggg tttcaattcc cctacgtcct tgtccgtggg
      301 aaagggcgtc ttggcgccct tgggattggg ctggtgaaat tgtggggatt tgtgaaccgg
      361 acgttttttc ataaccctcc acagcgtgtt ggtgcccaaa tcctttttga ttgctcgccg
      421 ggttctgatc ccttaaagaa ggggctggag aatgattgga ttcgtttgga ttatttaggg
      481 gagcgtaatt ctccagatgt gccgcaatga cggcgaggaa cttgggccca aactcctcta
      541 attttcgctt tccgactcca ctaatctgta gaaattcctg ttcattggtg ggacagtctc
      601 gtgccatctg ccgcaatgcc acatcggaaa atatgacgta agcggggacg tcagcttcat
      661 cggctaacgt ctttcggagt tggcggaggc gagtgaagag catctcgtcg tgacccaggt
      721 cctgaggaga cggggttttg cgctccggtg tgctcatggg cttggtgagc atgagcgtct
      781 tggggtcctg aagaaaatgc tgcccctgat cggtgacttc cagcaccgtg aaggggtctg
      841 ccgtttgttg aaggtacccc agccggatga gttcccggct gatggctgcc cattcgggcc
      901 gccgatgggc ttcccctttc ccatagctgg ttagccggtc atgaccccat cggcggattt
      961 tctcggtgtt ggctccggtg aggacggcga tgacgtgatt gagtcccacg ctaaatcctc
     1021 cggatccacg tatttcttca acgcaggtga gcacggtttg tgcggcattt gtgccgtcga
     1081 aggtctcacg gggtgtcagg cagttgtcgc atccgccaca gttttctgat tcaacatgct
     1141 ctccaaaata ttggagtagc gtgcgacgcc gacaggtgcc gcattccgca tattgcacca
     1201 tttcctgtaa ttgccggttg gcaatctgct gttcctggaa gttgggtttc tcattgatga
     1261 agcgttgctg cttgaccgta tccccggcgc taaagagcag gacgcactcg cttggcaggc
     1321 catcgcgccc ggcgcggccg gtctcctgat aataactttc gagatttttt ggcaaatcgt
     1381 agtgaatgac aaatcgcacg ttgggtttgt tgattcccat gccgaaggca atggtggcgc
     1441 agatgacctg gatttcatcc cgcaggaata attcctgatg ccgtgtgcgc tcctcggcac
     1501 tcaggcctgc atgatagggc tgtgcgttga tgccatggtc ctgtaatcgc tccgccacac
     1561 tttccgtggt ttttcggctc tggcagtaga gaattccgca gtccttgggg cgtgtccgga
     1621 gaaatgccag aacctgtcca aacgggttgt ccttaggctg cacctgataa gtcagattgg
     1681 gacggttgaa actggcgaca tagcatgccg gattccgcaa tcgcaggagg gtcacgatgt
     1741 ctttttgcac tcgcgccgtg gcggtggcgg tgagtgcgat gattggaagg gcagggacct
     1801 gatctcgtag ttccacgagc cgccggtatt ccggccggaa gtcatgcccc cattcactga
     1861 tgcaatgggc ttcatcaatg gccagcagat tgacctgaaa attccggatc aggttgaggt
     1921 acccggggag catgagccgt tccggtgcca tgtagagcag tcggtagtcg ccttggtaca
     1981 gcccccgcaa gcgggcctgg gattcctgcg cggtgagagt ggagttgaga taggtcgcgg
     2041 caatgccatt ggcccggagc ccatcgacct ggtctttcat cagggcaatg agcggtgaga
     2101 cgacgatggt taggcctgag cgggccagtg ccggcaactg aaagcacagg gactttcctc
     2161 ctcccgtggg taacagggct acgacgtcgc gctcggccag gacatcccga atgatctctt
     2221 cctgtaaggg tcgaaaggcg gtgaagccaa aatgttcctt tagcagagag agaaggggag
     2281 gttgtgttga ggtatgcagg ggcggggaac ccgtttgttg agggaatagg ttattcttca
     2341 caattgaagt tgtcgcaaat gttggagatg ttaatgaatc gtcttgatag aattattgag
     2401 gtgtgccgcg agggtcatct tgtggaggga gatcctccac tgacattagg tggcttcctc
     2461 atttcccact ccgatctatt tccttcggct caggcgcaat gttcagattt tttcttaatt
     2521 tcttggtcat ttcggcatgt tgttctttgt cagtgggcgt ctcaggatcc gccatcttgc
     2581 tctttccata aagaggctgt ctacctgtgt atgcacaaga tcctcgacat catgaggaaa
     2641 tctggggcat tcagcaagaa tcgctggaaa ttgccgaaaa gaacaaagaa accctttggt
     2701 gccataatgt atgtagccga acgaatgcaa gatacaaccc tcgtcttgat gatttcagga
     2761 cgagtgaccg tgtactccag gaaagttttc caggggatgg tgaaggcggc cagattctct
     2821 ggtgccaagc atatcatttt caatatgcag ggcgtcacct tcatggacag cattgccttg
     2881 ggtgggttgg tgctcgccta cctggacttg aatgagaatc atatggccat gagcgtcgtg
     2941 gagcctcaac atccggtcaa aaccttattc gaagacgcaa attttcccga tctgattccg
     3001 acgtatccca ccgaagagac tgccttgcaa gccatccaat agtggtctgt tgaaaaaagc
     3061 cgccaactgc gttttcgcca tttttccgta ctcacctact ccgtgtacgc tccgcgcgca
     3121 aaaatggctg tggccttccc ttccacataa ctcaggtcag gactggacga acttttcgaa
     3181 tggctcaggg catgcttttt tgaaccgatc cgaggcctct ggccttcaaa gttgccatgg
     3241 ggatttttgc acatggagaa cagtatgatt caacactccc aatagattta cgggtgcgct
     3301 tccctttttc atcgtttaaa atgaggtaag tcaaatttcc ctactcaggc gcgaacatgt
     3361 cgacccggcg catatccgag acgattcagg atgtgcacag atgtggtgtc ttccgggaat
     3421 gtgtgctttt acaatcggca gatcatggcg aatgtgttat cctcgcgtgg gtttgctagg
     3481 gaacaagtag aacctgaatt ctgagaataa taggaatgtt gtgatggcat atgaacatct
     3541 tctcgcgcta cgagcggagc tgctccgtga acaattaacc ggttttgtgg tgccccatac
     3601 ggatgaatat caaaatgaat atctgcctcc atgcgccgag cggcttgcct ggctaaccgg
     3661 atttaccggt tcggcgggga cggcgattgt cctgcgggat gaagccgcaa tgtttgtgga
     3721 tggccggtat acgcttcagg tggctgacca ggttgatgag aaaagctttt cgcttcacaa
     3781 tagtggagac acttcaccac atgaatggtt agcacttcgg gtgacaccaa cggatcgaat
     3841 cggctatgac ccctggcttc atactccgca ggatctcgaa cggtttcaga cagcaaccga
     3901 acgagtgggt gcacatctcg tcccctgtgt atcgaatcct attgatgcgg tctggcatga
     3961 ccggcctcaa caaccctcag gtgtgatcaa gcctcatctg ctggagtttt ccgggcaaag
     4021 ctcccaggct aagcgtgaga tatttggcag gagattctcg gaagacggga tcgatgcggc
     4081 catcatcacc gcgccggatt ccattgcctg gttgttgaac attcgcggct cggatgtgcc
     4141 ccatactccg ctaccattat gccgggcaat tctttacacc acggggcggg tcgccctatt
     4201 tgtcgatccg aaaaaaatca caccgggctt gcaagcgcat ttgggtccgg acatttcctt
     4261 ggcattgccg gaagaatttg aagccgcttt acaacagtta ggaacccatg gaaatcgagt
     4321 gctgtgtgat ccggccaaga ccaattcctg gatttttacc acattaactc agagcggggc
     4381 tgttctatta caaaaagatg atccctgtgt gctgccaaag gcctgtaaga atcctgtgga
     4441 aattgccggg gcgtatgccg cgcatcagcg ggatggagtg gccatgtgtc aatttctggc
     4501 gtggctggcc cgggaaggat ccaagggaca ggtgaccgag ttagaagccg tggactatct
     4561 tgatgcctgt cgtcgaaagc aggcgatgtg ggaggattgc agctttccga ccatttctgg
     4621 tgcggggtca aatggtgcca ttgtgcatta tcactcgacc cgggagacga atcggtgcct
     4681 ggagcccggg acattgtact tggtggattc cggcggtcag tatttggacg gaacgacgga
     4741 tatcacacga acgctcgcga ttggacgccc ttcggacgaa catcgtgatc gttatactcg
     4801 cgtgttacaa ggccatattg ctctggccac ggctagattt cctgaaggca cgacaggcag
     4861 tcaattagat gtgttggcac gtgcgcccct atgggcaatg gggttggatt acgatcatgg
     4921 gacaggccac ggagttggga gttttttagg agtccatgaa ggcccgcaac ggatcgggaa
     4981 agctgccaat gcggttgccc tgaagccggg gatgattgtg tcaaatgagc cgggctatta
     5041 caaaaccggg gcctatggga ttcgcctgga aaacctcgtg acggtggtga aagatgagtc
     5101 ctcccagaac tcaacgcggt cattccttgg ctttgatacg ctgacgatcg taccatttga
     5161 gcggaccctt attgccacag aggtgttatc gcttaaggaa cgccaatggg tcgataccta
     5221 tcatgcccgg gtgtgggcgg atctccaatc ccttgtggat accgatacgc gtgcctggtt
     5281 ggagcaggcg acgcaaccgc tcaactagtc cgtctcttcg cccgagacca ttcgaactat
     5341 aatcaaatcg gagctaacga tacatgacac cacaaaaaac gaatgaagaa atcactgaag
     5401 ccattaaaac tcaaatgggt aaaaatccag agattacgaa tgtgatcgtg aagggccatc
     5461 tgcttcaact gcatgtgacc caagggctat ttcatcgctt gtcggccgat cgcgagcgag
     5521 gtcggaaaat tgtcctggtc ctcatggaac agatgaagca actgaccgga ttaacggatg
     5581 ttgcagtgtg ggtctattca gagaacgaga aagtgattga gggaaccgtc aaagcctttg
     5641 gcggggataa tgtgaatttc ctgtttgact tgtaagtttt acaagagaac tccagggaaa
     5701 gggacgtacc ctattcctga taggtgccct tcggccagga gtccagacta ggaggaagtc
     5761 cggttttcca agatcccaca cgtttcccac cgagaccaat ttgacaggac cacactccgt
     5821 tcggcgtatg gttaatccgc catcgtcctc catcggcact gatccacatc cagaccgggg
     5881 aacgtcgaac gggccgttta gattcggatg gcacttaaac ctccttgtcg gcgtgctgaa
     5941 tgggactttt gtgggagttg aagtcaatat taccgagagt attgtgatcg attgctatga
     6001 ggcttgacaa gttccttgcc ggatatgtca tggtgtgccc atgaaaaatt cagattatcc
     6061 ctcatcagct attgtgccgg aattatccac aggcttggtg gggcagtacg aagcggatgt
     6121 tctgtgtctg tccgtatatg ggaagaccgt gatcctgatt ggcacggcgc atgtgtcaca
     6181 agaatcagtg gatttagtca aactcgtcat tgaacaggag catccggatc gtgtctgtgt
     6241 agaattggat acgaagcgtt ttgaagccat ctcacatccc aaccggtggg aatcgttaga
     6301 cctcaaagaa atcatccgca agcagcagct cagtacgctg atggtcaatc ttattttgtc
     6361 ttcctttcaa aagcggctgg gcgataaatt gggcgttatt ccaggcacgg aaatgctgga
     6421 ggccattcgt atggccgaca agcatgggat tcccgtgacc cttgctgacc gggacgtgcg
     6481 ggtgacgatg cgtcgggcct ggcgcaatac cccaatgtgg cgaaaaagtc tcttaatgtc
     6541 ctccctcatg ctgagcatct tcgacaccac tgaagtctcc gaagaggaga ttcggaatct
     6601 caaaaagcag gatgtgcttt cagaaatgat gaaggattta ggaaaagaag tgccgacgct
     6661 caaaaccgtg ctcattgacg agcgggatca ttatttggcc aaaaaaattg tcgatgcgcc
     6721 tgggacccgg attgttgccg tggttggcgc cgggcatgtg gaagggattt gccgaacatt
     6781 gaacgaacgg gagacggtgg tcttggagga gctggaaagt attccgcctg tctctcctgt
     6841 gtggaaagcc gtgggttgga gtatccctgt gttgattgtg gggtcaattg cctggattgg
     6901 atgggaaaaa ggtttaagtg ctgccggaga caatttattg ttttggattt tggccaatgg
     6961 tattcccagc gggattggcg ggttgt
//
LOCUS       DAORAQ010000135         6912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1653_length_6912_cov_69.292985, whole genome shotgun sequence.
ACCESSION   DAORAQ010000135 DAORAQ010000000
VERSION     DAORAQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_6912_cov_69.292985"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKM72_10805"
     CDS             complement(<1..196)
                     /locus_tag="PKM72_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61323.1"
                     /translation="MLGMNRDLLGNYKNDVGIESNHFACVAWTNRWKLGKIEKASNHE
                     TCVILTEIRCCVSETKPCPSC"
     gene            315..1382
                     /locus_tag="PKM72_10810"
     CDS             315..1382
                     /locus_tag="PKM72_10810"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNP61324.1"
                     /translation="MTPTSFRVLSVIGAGAWGTALAHLLATKGFSIRLWAHEPEVAET
                     IQRTRENSWYLPEVVLPNSIQATISLPDCVQGTDVILLAAPSHAMANMVKQLNLLLKE
                     PLPIIIATKGIEEGTLQLMSQVVESHLPASWHPFITILSGPSFASEVSRWKPTTILLA
                     GRDFNLVNGLQQAFITPQFRVYAGRDMIGAQLGGALKNVMAIGAGVVDGLDLGSNARA
                     ALITRGLAEMIRLGRAMGADVSTFYGLSGLGDLVLTCTGTLSRNYQVGMQLAKGANMT
                     TVRSTTRTVAEGVPTSRAAMALAERYHVDMPIVRGVFQMLFEGRNPRHIVTDLMSRLA
                     KGETDGLFSVSTATFGPRAHS"
     gene            1379..2149
                     /gene="larB"
                     /locus_tag="PKM72_10815"
     CDS             1379..2149
                     /gene="larB"
                     /locus_tag="PKM72_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNP61325.1"
                     /translation="MNHDRLAALLQRVQGGHVSVPQAIQQLRTLPFEDLGFASVDHHR
                     SLRQGFPEVILCEGKTPAQITAIARKLLKAGGPFLATRVTPANARSLKRLARKAVYHE
                     MARMVSLAESKRTRQGLILIVTAGTSDIPVAEEAKVTAEVMGSRVQTLYDVGVAGLHR
                     LLDRQESLHQARVVVVVAGMDGVLPSVVGGLVDRPIVAVPTSQGYGANFGGLAPLLTM
                     LNACSSGIGVVNIDNGFGAGCLAHRINISGQGESPPQT"
     gene            complement(2115..2975)
                     /locus_tag="PKM72_10820"
     CDS             complement(2115..2975)
                     /locus_tag="PKM72_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61326.1"
                     /translation="MSSTLRKFQWRWQSGLLVLALGSFLALSPFSSSEAKSKRVSVSP
                     VKITDVVVSPDPFIIGKSGLKLTLLVELPSSLRGANVLEVSAMITSPTRRSMSFVAHR
                     LLVDEPEAKRGNLTVPVELVWDGKDQYHQLVADGSYFYELQAKLMEDQGDGPRTKIVS
                     RRVHGTLEALAYVGEVLPPLPPEPDIPEELEILREGENSEENGTATEESLKSSDDPVV
                     PEEEALMDATDVLQSEEAGGSGSEVESVEPEEKDESTIPEMGEAPMSEPLNEPMPELP
                     TSEEEIPPAR"
     gene            3371..4600
                     /gene="larC"
                     /locus_tag="PKM72_10825"
     CDS             3371..4600
                     /gene="larC"
                     /locus_tag="PKM72_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNP61327.1"
                     /translation="MTRHLHIDAFSGVSGDMFLGALVDTGVPLSVLKKGLKALGIKGY
                     RLREQQVIRNSIRATKVDVDIQKGFTKPLSLATIKKTLKNSPLPDAIRTQALQTFQLL
                     AEAEGRVHGQELNKVHFHEVGVIDSLVDIVGTLLGFAHLNVTTVSFSPINLGAGTIST
                     AHGLLPVPGPAVAHMAQGIPILSNGPAIEFTTPTGIALVKTLSQDCKPLPPLTPQTVG
                     YGAGTADPHGWPNVLRLFVGAQDSDSKNLTERIIQLETNIDDMNPQLYGVIMERLFEA
                     GALDVTFTPTIMKRSRPGTIVTVIAFSQNLQALKSILFSETSTLGIRIQEMDRAILAR
                     SFQTIKLLGGSVLMKIANLGQGRSKATPEYRDCVALAERTKQPVQTIIDLARQTYISA
                     KIPSPRKTAPSKLARRK"
     gene            4608..5633
                     /gene="pdxA"
                     /locus_tag="PKM72_10830"
     CDS             4608..5633
                     /gene="pdxA"
                     /locus_tag="PKM72_10830"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNP61328.1"
                     /translation="MSSSQPTKPLLAITMGDPAGIGPEIIVKALQLSKVWRVCRPLVI
                     GSRAILELTTQSLGASLVLVPVSGHESSPHPRLFRRGSLPFLDPFSGSSRPVRLGRVT
                     ARSGDLAVTCIQTAVRLAQAGCVQGIVTAPINKHAIHLAGHTYPGHTEMLADLTNAKE
                     SGMMIVGGPLKILFATTHLALREVPNVLTPATILRAIRLAHQGLTRLFHIQKPRIGVA
                     GLNPHAGENGLFGDEEDRVIQPAIRQAKKQGIACTGPHPADTLFGKAVRGSFDGIVAL
                     YHDQGLIPLKTVAFGHCVNITVGLPIIRTSVDHGTAYDIAGHGKADPTSLVEAIEMAA
                     NLATRST"
     gene            5650..>6912
                     /gene="rsmB"
                     /locus_tag="PKM72_10835"
     CDS             5650..>6912
                     /gene="rsmB"
                     /locus_tag="PKM72_10835"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNP61329.1"
                     /translation="MGARQSTSSAKPPASSTGRAWSARKIAAATLQAIERSRRFSDDV
                     FDHITKDLVVSERDRHLAFELVYGVLRHYITLDWRLDQVSRKAMVRLPLTVATTLRVA
                     AYQLLYLDRIPASAAVNEAVQLIRKQPGHNWSGFVNAVLRNLLRQSAPPMPDPGVDAT
                     QALSIRYACPPWLVERWRESFGLMKAEHLCQQSLGLPPLTIRTNSLRGSRSDLLACLQ
                     SEGILAQETTVSPVGLTLDKCGNPGQLSALKDGWCYVEDEAAQLIPLLLDPQPGDRVL
                     DACAAPGGKTTHMAQMMKNQGTIVALDRHQGRLDRLVSNCVRLGISIVQPAHVDLSPS
                     SGHGPADDSSTTPLRVPAMLAQPFDRILVDAPCSALGILRRHPEGKLLKSLSTIQHAQ
                     ASQREILDRVSDLLRPGGILVYSACSVEP"
BASE COUNT         1539 a         2020 c         1741 g         1612 t
ORIGIN      
        1 cacaagaagg acacggcttg gtttcactca cacagcacct gatttctgtt aagatgacac
       61 atgtctcatg attgctggct ttctctatct tgcccaattt ccatcgattc gtccaggcga
      121 cacaggcaaa atgatttgac tcaattccca cgtcattttt ataatttccc aacagatcac
      181 gattcattcc caacatcgac cctcatttgt tccctcagat tggtgcacgc ttctctccct
      241 attcatccgt tcgccccctt ggcccatgca ccaactccac aatcatccat gaaccacctt
      301 tgtcaggaga caccgtgaca cccacttcct ttcgtgtcct ctccgtgatt ggcgctggag
      361 cctggggaac cgcattggct cacctcttgg ccactaaagg attcagcata cgcttatggg
      421 cgcatgaacc cgaggtggcg gagaccatcc agcgcaccag agaaaattcc tggtatctcc
      481 cggaagtagt cctacccaat tctattcagg ccacgatcag tcttccggat tgcgtgcagg
      541 gcacggatgt catcctactc gcagcaccat cccatgccat ggccaacatg gtgaaacaac
      601 tcaacctgtt attaaaggaa ccccttccca tcatcatcgc caccaaaggc attgaggaag
      661 gcacgttgca attaatgagt caggtagtcg aaagtcatct ccccgcatca tggcatccgt
      721 tcatcaccat tctttccggg cccagcttcg catcggaagt gagccgatgg aaacccacga
      781 ccattttact ggccggacgg gatttcaatc tggtgaatgg tctgcaacaa gcctttatca
      841 ctccacagtt tcgcgtgtat gccggacggg atatgatcgg tgcgcaactg gggggagcct
      901 taaaaaatgt catggccatt ggagctggtg tggtcgatgg cctggacctc ggatctaatg
      961 cgagggctgc cttaatcacc agaggactgg cggaaatgat ccggttaggc cgggccatgg
     1021 gcgccgatgt ttccaccttt tacgggctat cggggttagg agatctggtt ctcacctgta
     1081 ccgggacgtt gagccggaat taccaggtcg gcatgcaatt ggccaaaggc gcgaatatga
     1141 ccacggtgcg ctcaaccacc agaaccgtgg ctgaaggggt tcccaccagc cgggccgcca
     1201 tggccctggc tgaacggtat catgtagata tgcccattgt gcgcggcgtg ttccaaatgc
     1261 tgtttgaagg gcgcaatccc cggcatatcg taaccgatct gatgtcccga ttagccaaag
     1321 gcgaaacgga tggccttttc tctgtcagca ccgcaacctt tggccccagg gctcattcat
     1381 gaaccacgac cggttagcgg ccttgcttca acgggttcaa ggcggacatg tttctgtccc
     1441 ccaggctatt caacagctcc gcaccctccc gtttgaagat ctcggatttg cctcggtgga
     1501 tcatcaccgg tcgctacgac agggatttcc ggaagtgatc ctgtgcgagg ggaaaacacc
     1561 cgcacaaatt accgcgatcg ctcgaaaatt attaaaggcc ggcggtccgt ttctcgcaac
     1621 acgcgtcacg cctgctaatg ctcgcagtct caagcgattg gcccgcaaag cggtatatca
     1681 tgagatggcc aggatggtct ctcttgccga gagcaagcgt accaggcagg ggttgatcct
     1741 tattgtcaca gccggcacct ccgacatccc ggtggctgaa gaggccaaag tcaccgccga
     1801 agttatggga agccgggtac aaacactcta tgatgttggg gtcgctggtt tacataggct
     1861 tttggatcgc caggaaagcc ttcaccaggc acgagtcgtc gttgtcgtcg caggaatgga
     1921 cggggtgttg cccagcgtgg tgggagggtt ggtcgatcgc ccgattgtcg ccgtgcccac
     1981 cagtcaaggc tacggtgcaa attttggcgg gctggcccct ctcctcacga tgctgaatgc
     2041 ctgctcatcg ggaatcggcg tggtgaatat cgataacgga tttggagcag gctgtctcgc
     2101 acaccgcatt aatattagcg ggcaggggga atctcctcct cagacgtagg taactcaggc
     2161 atcggctcat tcaaaggttc cgacattggt gcttctccca tttcagggat tgtactctcg
     2221 tctttctcct ccggttcaac gctttcaacc tcggacccgg aaccaccggc ctcttcagac
     2281 tgaagaacat ccgtagcgtc catgagggcc tcttcctctg gaaccaccgg atcgtctgaa
     2341 gacttcaagc tctcctccgt ggccgtgcca ttctcttcag aattctctcc ctcacggaga
     2401 atctcaagtt cttcgggaat atccggctcc ggaggaaggg gaggtaacac ttcacccaca
     2461 taggccaacg cttcgagtgt cccgtgaacc cgacgtgaga cgatcttcgt ccgggggcca
     2521 tccccctgat cctccatcaa tttggcctgg agttcataaa agtaagagcc atcagccacg
     2581 agttgatggt attgatcctt cccatcccag accaactcca ccggaacggt gagattcccc
     2641 cttttcgcct ccggttcgtc caccaatagg cgatgtgcga caaaactcat ggagcgccgc
     2701 gtcggtgagg taatcatggc agacacttca agcacattgg ctccgcgtaa cgaggacggc
     2761 aactccacga gcagggtcaa tttcaaccca cttttcccaa tgataaaggg atcaggagaa
     2821 accactacat cagtaatttt cacgggagaa accgaaaccc gtttggactt cgcttcgctt
     2881 gagctgaagg gggaaagagc cagaaatgac ccgagggcca gcaccaacaa cccagactgc
     2941 caccgccatt gaaatttcct aagtgtcgaa gacatcctta tcattgtgcc aaactttttc
     3001 ccttgagacc aatctaattt gagattaatt ctgccatttt ttttctttgg cctttggcgt
     3061 gcccgagccg tcaagccgat ttcggaagca cctcggcgca ctgcttaagt ccttcgacaa
     3121 ggttcaggac aggctccgca agtttgcgcc gcagccggaa ttggtgaacc gcacgatgaa
     3181 cccgaagggc cacgaaaggg ccaatatggt tttgggtcct tttgccgtca caaaaggacc
     3241 tcgtacgccg gagcgaaacc cggataattt caaaaacatt atcgtgcaat tctcaatgat
     3301 tcgcccagcg tcgcgttgag atttttgata tgctacggtt tcgcctttgt acttttgcag
     3361 atgtaattct atgaccagac atcttcacat cgacgcattt tcaggagtca gcggggacat
     3421 gtttttgggt gccctcgtag ataccggtgt accattgtct gttcttaaaa aaggcctcaa
     3481 agccctaggc attaaaggtt atcggcttcg tgaacagcag gtgatccgca acagcatccg
     3541 ggccaccaag gtggatgtgg acatccaaaa gggatttacc aaacccttat cacttgccac
     3601 catcaaaaaa acgttaaaaa atagtcccct gcccgacgcc attcggactc aggcccttca
     3661 aaccttccaa ctgctggctg aggctgaagg cagggtgcat ggccaagagc tgaataaagt
     3721 acattttcat gaggtgggcg tgatcgattc gctggtggat attgtcggga ccctcctggg
     3781 atttgcccat ctgaacgtga cgacggtgtc attttctccc atcaatctgg gagccggcac
     3841 gatttccacg gcgcatgggc tgcttccggt accgggaccg gcagtggcgc atatggccca
     3901 aggcattccc attctctcca atggaccggc cattgaattc acgactccga ccggtatcgc
     3961 gttggtcaaa actctctctc aggattgtaa accgctccca cccttaacac cacaaacggt
     4021 ggggtatggc gcgggcacgg ccgaccctca tggctggccc aatgtgcttc gactgtttgt
     4081 tggtgctcaa gattctgatt ctaaaaatct tacggaacgc atcattcagc ttgagacaaa
     4141 catcgatgat atgaatccgc aattgtatgg ggtgatcatg gagcgcctct tcgaagccgg
     4201 agctctcgat gtcacattca cgccaaccat catgaaacgg agtcggcccg gcacgattgt
     4261 gaccgtcatc gcgttctcac agaaccttca ggccttaaag tcgattctct tttccgaaac
     4321 ctcgacgctg ggaatacgga tccaggaaat ggaccgagcc attctggctc gctccttcca
     4381 aacgattaaa ctgttggggg gttctgtcct catgaagatt gctaatcttg gacagggccg
     4441 ttcaaaggcc actcccgagt atcgtgattg tgtcgcattg gctgagcgca ccaagcagcc
     4501 tgtgcaaaca atcatcgacc tcgcacgtca gacctatatt agtgcgaaaa tcccatcccc
     4561 ccgcaaaacc gctccttcca aattggccag gcgcaagtaa cccacgcatg tcctcctctc
     4621 aaccgaccaa accgcttctg gcgattacca tgggcgatcc tgccgggatc ggaccggaaa
     4681 ttatcgtgaa ggcgctccag ttatcgaaag tctggcgagt ctgtcgcccc ctggtcattg
     4741 gtagccgggc catcctggaa ctcaccacac aatcattagg ggcctccctt gttcttgtgc
     4801 cggtgagcgg tcacgaatca tcccctcatc ctcgcctgtt ccgccgtggc tctcttcctt
     4861 tcttggatcc cttttccgga tcgagtcgtc cggttaggct tggacgtgtg acggcacggt
     4921 ccggggattt ggcggtcacg tgcatccaaa cagccgtccg attagcccag gccggttgcg
     4981 tgcagggcat tgttacggct cccattaata aacatgccat tcatttagcg gggcacacgt
     5041 atccgggcca taccgaaatg ctggcggacc tgacgaacgc caaggagtcg gggatgatga
     5101 ttgtgggcgg tcccttgaaa attctatttg caaccaccca tctggctctc cgtgaagttc
     5161 cgaacgtgtt gacaccagcc accatccttc gagccattcg attggcccat cagggactca
     5221 cccgcttgtt ccatattcag aaaccgcgca tcggcgtggc gggcctcaac ccccatgccg
     5281 gcgaaaatgg tctgtttgga gacgaggaag accgcgttat tcaaccggcc attcggcaag
     5341 ccaaaaagca gggcattgcc tgcacgggtc ctcatcctgc cgacaccttg ttcggcaagg
     5401 cggtacgagg atcatttgac gggattgtcg ccctgtatca tgaccaggga ttgattcccc
     5461 tcaaaaccgt ggccttcggc cattgcgtga acatcacggt gggcctgcca atcattcgca
     5521 catctgttga ccatggaacg gcctatgaca ttgccgggca tggcaaagcc gaccccacca
     5581 gcttggttga ggccattgaa atggcagcca atttggccac acgctccaca tgatatccaa
     5641 ggcgtaaaga tgggagcgcg acaatcgaca tcctccgcca aaccaccggc atcttcgacc
     5701 ggtcgtgcct ggagtgcgag aaaaatcgct gccgccacgt tgcaagcaat tgaacgatcc
     5761 cgaaggttta gcgatgacgt gttcgatcac atcaccaaag acctcgtggt ctccgaacgc
     5821 gatcggcatc tggcgtttga gttggtctat ggagtgttac gacattacat caccctggat
     5881 tggcgattag atcaggtctc ccgcaaggcc atggtccgcc tgccgctgac ggttgccacg
     5941 acgcttcggg tcgctgccta ccaattgctc tatctcgatc gcattccggc ttccgccgca
     6001 gtgaatgaag ctgttcagct catccggaaa caacccggcc acaattggtc cggcttcgtg
     6061 aatgccgtat tgcgtaatct gcttcggcaa tctgcccccc ccatgcccga tccgggggtc
     6121 gatgccaccc aggccctctc cattcgatat gcctgcccgc catggttagt tgaacgctgg
     6181 cgagaatcct tcggccttat gaaagcggaa cacctttgcc aacagtctct tggccttcca
     6241 ccattaacca ttcgaaccaa ttcccttcgg ggctcccgtt ccgatttact tgcctgcctc
     6301 caatcggaag gcatccttgc acaggaaacc acggtgagcc ctgtcggcct gaccttggac
     6361 aaatgtggta atcctggaca attatctgcc ttgaaggacg gttggtgcta cgtggaagac
     6421 gaagccgccc aactgattcc cttgctcctt gaccctcagc ccggcgaccg ggttcttgat
     6481 gcttgtgcgg ctcctggagg caaaaccacg cacatggctc aaatgatgaa aaaccaaggg
     6541 accatcgtgg cactcgatcg tcatcaagga cggctggacc ggcttgtttc taattgtgtc
     6601 agactgggga tctccattgt tcagccagct cacgttgatc taagcccctc ttccggccac
     6661 ggcccggctg acgattcttc aactactccc ctcagggtcc cagccatgct tgcgcaacca
     6721 tttgaccgta ttctggtcga tgcgccttgt tctgctctcg ggattcttcg ccgacatccg
     6781 gaaggaaaat tactaaaatc gttatcgacc atccaacatg cccaagcttc tcaacgcgag
     6841 attctggatc gggtcagtga cctcttgaga ccagggggaa tcttggtcta tagtgcctgc
     6901 tcagtcgaac cg
//
LOCUS       DAORAQ010000136         6894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1665_length_6894_cov_70.659307, whole genome shotgun sequence.
ACCESSION   DAORAQ010000136 DAORAQ010000000
VERSION     DAORAQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_6894_cov_70.659307"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKM72_10840"
     CDS             complement(<1..672)
                     /locus_tag="PKM72_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNP61330.1"
                     /translation="MIVQPITIQGHIIDSLILAKVLDAIVMLGGTFTLSEVTVGTRRE
                     DTSHATILIEAPTMELLQEILKTIQPHGAVVETEEDCTVEPAPADGILPEDFYATSHL
                     ATQIRWQGSWIEVPQPEMDVAIRLKPSPLSAQMVSMGSVKKGDLVVTGRKGVRVFPLE
                     RPKERDVFGFMEAQVSSERPHHHIIADVAKRMKAIQQQRKEGKESGKVLFAGGPAIIH
                     AGGREA"
     gene            895..1524
                     /locus_tag="PKM72_10845"
     CDS             895..1524
                     /locus_tag="PKM72_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61331.1"
                     /translation="MNHHLCTVLLIAGLAGGYGEEVLQTTAWASSGTDVFQSFTQHIG
                     AQIGKDKQEFAIADSCTVYFYQHLKKKPRPEVERIHFSPISSKTGLDYDCGKLFPEGL
                     EQARQAFGNMQQRLSLSLTFYQMALVGDGDDNQEYSAQEIQDVLAAFGLPYLDGLPHG
                     GYVIALTGLFDTIRQDLQFKILMEGMQELMNKGYRFTKADQAALNRKLN"
     gene            complement(1548..2183)
                     /locus_tag="PKM72_10850"
     CDS             complement(1548..2183)
                     /locus_tag="PKM72_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP61332.1"
                     /translation="MATQPEATRSINLFFGLLVLGLIVAGIWTWHHLTFDTQDYITEE
                     ILPILGGLLVLGVSIWIGTKKWQARKERLQLRDRLLQRFQKEPSAQKRRDLAFTLIEV
                     NQFEVEGLETVSEPMAELFIWTLKTAIGDKQHRIRGMSASYLGILKHQPSIPLLIRAL
                     EDEHAHVRACAALALGRMRAQDAKAKLEEKMKEDWDQTVRSRSQEALERIR"
     gene            2232..>2470
                     /locus_tag="PKM72_10855"
     CDS             2232..>2470
                     /locus_tag="PKM72_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNP61333.1"
                     /translation="MKVTILGSGTNIHPTRAAAGYVVETDRPILLDFGPRTLSNLLQT
                     GIDRHTIQYLLFTHFHADHFSDFIPFFFDAVCHSK"
     assembly_gap    2471..2570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2571..2783
                     /locus_tag="PKM72_10860"
     CDS             <2571..2783
                     /locus_tag="PKM72_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61334.1"
                     /translation="SHQPGGSHMHAEECGKVAEKANAKKLVLSHFYEAADRSNVVAQA
                     RRFFSGPIIKGKDLLTFQIAKASKVA"
     gene            2913..3758
                     /locus_tag="PKM72_10865"
     CDS             2913..3758
                     /locus_tag="PKM72_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61335.1"
                     /translation="MPTWAKMVLGPFAPQQRLRLSGGASENSAAAPPPLPSAMGKPQH
                     MKEKKNVRRGKLPKTDRNFKLMLRATFLLDQIEDFCGTSSCKEKFFWKIKLIPSRYFG
                     TGLEYFKLCNVSITELIFYKGVFMIHISKSLTSILGTMFLVMFGSTLAVAGSTINVTI
                     KDNGYHILGSSSGTRAPGFSMAAGEENVIILKNENHTNHSFVSKAFKAMDVVVTGEAE
                     GVKDGDATGWIVEPGKSIQLKFIPPVGKDFSGSWDVFYCTIHGKDKMMGEIVVADTRG
                     GAGAF"
     gene            complement(4117..4989)
                     /locus_tag="PKM72_10870"
     CDS             complement(4117..4989)
                     /locus_tag="PKM72_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNP61336.1"
                     /translation="MHSPSETKIQALLRLLSDPNEQVAKTIQQEFVRIGPSALPFLEK
                     ARADDTALESRVAFVKDEIRFEQLKEEFSTFVAHCSRQIDWEQGAFLIAKIAYPDVDI
                     PHYQECLDLLTEEFRKKWHSEEAPPGKVARLLSAFLFKDKGFSGNRIHYHDPDNSYLH
                     RVIESRQGIPISLSAIYVFVGNRLNLPLSGVGMPGHFLVKIEGEPIPQFVDCFNGGAF
                     LREQDCEQFITASGLDYTPEFLEKSSTRLILARMLRNLLSIYEREERNPMTGRIQTLL
                     QVLDESSTDEDPSL"
     gene            complement(5077..5856)
                     /locus_tag="PKM72_10875"
     CDS             complement(5077..5856)
                     /locus_tag="PKM72_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP61337.1"
                     /translation="MNEQIQEVLALTNRWVKRLSREKFSMLFTLIQPMLFWLIFFGNL
                     FQRAADVQVVQAPNYMSFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMATPIRR
                     SSVILSRFLFVMAITAMQILVILGVAFLFGVSAETGILGIALILVIGMLFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPGWMKSLAFFNPMTWVIDAIR
                     PLILTGWSEALPHVAIALVVLAVFDGLCLYGGARAFKRSVG"
     gene            complement(5900..6874)
                     /locus_tag="PKM72_10880"
     CDS             complement(5900..6874)
                     /locus_tag="PKM72_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNP61338.1"
                     /translation="MSDAVAIEVKGLGKTYDTVTAVKDLSLSVTQGEIFGLLGPNGAG
                     KSTTLRILITTLSPTSGTATIFGHDVVKEADTIRRLIGYVPQERAIDRFLTGREHLLL
                     LADLYHLPKNEALTRIASLLKLVDLEEHADRVAKGYSGGMKRKLDIACGLLPNPKVLF
                     LDEPTLGLDVQSRLNIWQYLRQLREQGMTIVMTTNYLDEADQLCDRIAIIDRGEIRAI
                     GSPKDLKASLGGDGVWLTLGDPAPQVVETLSTALKALPFVRAIRPTPQGLDIRVDAPE
                     KALPAILETTNRVGCRLDGIEYHRPRLDDVFVAHTGRSLTDQPPEPTT"
BASE COUNT         1695 a         1658 c         1762 g         1679 t
ORIGIN      
        1 ggcttcccgt cctcccgcat ggatgatggc cggaccaccc gcaaacaaca ccttaccgga
       61 ctctttgccc tcttttcgtt gttgttgaat ggccttcatc cgtttggcca cgtcggcgat
      121 gatatggtgg tgagggcgtt cggaagaaac ctgggcttcc atgaatccga acacatcccg
      181 ttcttttggt cgttccagcg ggaatacccg aactcctttt ctccccgtga cgaccaggtc
      241 gccttttttc accgagccca tggaaaccat ctgtgcggaa agtggagagg gcttgagtct
      301 gatcgccaca tccatttcgg gttgcgggac ctcgatccag cttccttgcc accgtatttg
      361 agtggccaga tgagaggtcg cgtaaaaatc ttcaggtaaa attccatcag ccggggctgg
      421 ctcaaccgtg cagtcttctt cggtttcaac cactgcccca tgcggctgaa ttgttttcag
      481 gatttcctgt aataactcca tggtgggagc ttcaatcagg atggtggcat gggatgtgtc
      541 ctcccgccgg gttcccactg tcacttccga gagggtaaag gttcccccca acatgacaat
      601 cgcatccagg acctttgcta agatcaatga atcaatgata tgcccttgga tggtgatggg
      661 ttgaacgatc atttttttat tttaccatga aattctgcaa aggcgtgaag tgaaaatcgg
      721 ttggggttta ttagcctcgc atgatgttga aaaggtgttt ggatagtgtt cgctcaggac
      781 gacatggcaa tgcctatcag cccaacataa aggaaatcgc agaagtatca ttctcatgcg
      841 tttccttaca gtgctatact atgaccatct atcatttacc agggggaatc aagattgaat
      901 catcatcttt gtactgttct tctcattgct ggactcgcag ggggctatgg ggaggaggtc
      961 cttcagacta cggcctgggc ttcttccggc acggatgtct ttcaatcttt cactcaacac
     1021 atcggcgcac aaatcggaaa agataaacag gaatttgcca tcgctgattc ctgtacggtc
     1081 tatttttatc aacatttgaa aaagaaacca cgcccagaag ttgagcgcat tcatttctcc
     1141 cccatctcct caaaaacagg gcttgattat gattgtggga agctctttcc cgagggactg
     1201 gagcaggccc ggcaggcctt tgggaacatg caacagcggc tctccctcag cctcaccttc
     1261 tatcaaatgg ctttagtggg agacggggat gataatcagg aatatagtgc gcaagaaatt
     1321 caggatgtgc tggcagcctt cgggctaccg taccttgatg gacttcctca tggagggtat
     1381 gtcatagccc tgacgggtct gttcgatacc attcgccagg accttcaatt caaaatcctg
     1441 atggagggga tgcaggagtt gatgaacaag gggtatcgct ttaccaaagc tgatcaggcg
     1501 gcattgaatc ggaagctgaa ttgataggtc atggaggggg aagcgttcta tcgtattcgt
     1561 tctaacgctt cttgtgagcg gctgcggaca gtctggtccc agtcttcttt catcttttct
     1621 tccaatttcg ccttcgcgtc ttgagcccgc atgcgcccca gggctaatgc ggcgcaggct
     1681 cgcacatggg catgttcatc ttccaacgcc cggatcaata aagggatcga gggttgatgt
     1741 ttcaagattc ccaggtaact ggctgacatt ccccgtatgc gatgttgttt gtcacctata
     1801 gccgtcttca acgtccaaat aaagagttcc gccatcggtt cggaaacggt ttccaatcct
     1861 tctacttcaa attggttcac ttcaataagg gtaaaggcga gatctcgccg tttttgggct
     1921 gagggttctt tttgaaagcg ttggaggagc cgatcacgaa gctgaaggcg ctctttgcga
     1981 gcctgccatt tttttgtgcc aatccatatg cttacgccca aaacgagaag gcctccgaga
     2041 atcgggagga tctcctcggt gatgtaatcc tgagtgtcga aggtcagatg atgccatgtc
     2101 caaatgcctg ctacgatgag ccccagaact aacaggccga aaaacaggtt gatggaacgg
     2161 gttgcttcgg gttgggttgc catacggggg gtagtcatca gggagttgtt atttaccggc
     2221 aaggtcgaac aatgaaggtc accatactag gttcaggaac gaatattcat cctacccgcg
     2281 ctgcggccgg gtatgtagtt gagacggacc ggcctattct actggatttt ggtccgagaa
     2341 cgctctcaaa tttattacaa accggcatag atcgccatac gatccaatac ttactcttta
     2401 cccatttcca tgcggaccat ttttctgatt ttattccatt tttctttgat gcggtgtgcc
     2461 attcaaagtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctcatcagc
     2581 caggtggatc gcatatgcat gcagaagaat gtggaaaagt agcggaaaag gccaatgcta
     2641 aaaaacttgt gctttcgcat ttttatgagg cagccgaccg gtcgaatgtt gtggcccaag
     2701 cccgccgatt tttctcaggc cccatcatta aaggaaaaga cctcctgacc ttccagatag
     2761 caaaggcctc caaggtggcc tagacttgag ggcgatccta taaccctcgc ctgcaggtcg
     2821 gccaaacggg ctgaggctca aagcaaaaag cggaccttgt ccgatccgcg agttggtccg
     2881 cccctccgta gcttgcgtca gcacgattcc atgtgccgac ctgggcgaaa atggttttgg
     2941 gtccttttgc tccacaacaa aggttgcgcc tttcgggagg cgctagtgaa aactcggctg
     3001 ccgctccgcc cccccttcca agtgccatgg gcaaacccca acacatgaag gagaagaaaa
     3061 atgtcaggcg gggaaaacta ccaaaaacag accgcaattt taaactgatg ctaagagcga
     3121 catttctcct ggaccaaatt gaggattttt gcggcacttc ctcatgcaag gaaaaatttt
     3181 tctggaaaat caaattgatt ccttctaggt attttggcac aggtcttgag tatttcaagt
     3241 tgtgcaatgt ttcaataacc gaattaatct tttacaaagg agtctttatg attcacattt
     3301 ctaaaagcct aacttccatt ttgggcacaa tgtttttagt aatgtttgga tccacgctgg
     3361 cggttgcagg ctccacgatt aatgtcacga ttaaggacaa cggctaccat attttaggta
     3421 gctcctccgg gacacgggct ccaggttttt ccatggcggc tggtgaggaa aacgtgatca
     3481 ttttaaaaaa cgaaaaccat actaatcatt cctttgtctc gaaagcattt aaggcaatgg
     3541 atgtagtggt cactggagaa gcggaggggg taaaagatgg agatgccacc ggttggatag
     3601 tggagccagg caagagcatc cagctcaaat ttatccctcc ggtggggaaa gatttttcag
     3661 gcagctggga tgttttctat tgcaccattc acgggaaaga caaaatgatg ggtgaaatag
     3721 tcgttgccga taccagagga ggagctggcg ctttttgatc ttcaatcagg atgttgaaac
     3781 caatcgggtc gctggaataa taaatttcag cgaccctttt ttatttatac caatgtattg
     3841 caaatggggg gtacccagta gcaacttctg acaagtgagc acaccactcc tctctcgttt
     3901 gttcttttgc taaagccgtc tgcttttctc tgccgtgcgg cctccgattc gggtctgggt
     3961 cttcatggat gaggcgcgcc gaaatccggc atttccaagt gccaagggca aattccacaa
     4021 tggatgagag gctcaatatc aggcggagga aaaatctcaa caaaggatta gaacccgtta
     4081 gtgaggtaaa ggactaacgg atatgacctg tcgtggctag agggaaggat cctcatctgt
     4141 ggaagattcg tccagaactt gaagcagagt ttgtatccgc cccgtcatgg gattccgttc
     4201 ttcccgctca taaatactca acagattccg gagcatgcgg gcaagaatca gacgcgtcga
     4261 actcttttct aaaaactcag gagtatagtc caaaccggat gccgtaataa attgctcaca
     4321 gtcctgttcc cgtaagaatg ccccaccgtt aaaacaatcc acaaattgcg ggataggctc
     4381 tccttcaatc ttcaccagga aatgtcccgg cataccgact ccggagaggg gcaaatttaa
     4441 acggtttccc acaaacacat agatggccga gagagaaatg gggatccctt gccgggattc
     4501 gatgacccga tgcagatagc tattatcggg atcatggtaa tggatgcggt tgccggaaaa
     4561 tcccttgtct ttaaagagga acgcgctgag gagtctggcc actttccctg gaggagcctc
     4621 ttcggaatgc catttttttc gaaattcttc cgtgaggaga tctaggcact cctgatagtg
     4681 ggggatatcg acatccggat aggcgatctt ggcgataagg aatgctccct gctcccaatc
     4741 aatctgcctg gagcaatggg caacgaacgt gctgaattcc tctttgagtt gctcaaatcg
     4801 aatttcatct ttcacaaaag caacacggga ttccaatgcc gtatcatctg cgcgggcttt
     4861 ttccagaaac ggtaaggccg agggaccgat tcggacgaat tcctgttgaa tcgttttggc
     4921 gacctgttcg ttgggatcgg acagcaaacg aagcagggct tgaatttttg tctctgaagg
     4981 tgagtgcaag aagttttgtc ccgggtagat acgacaacgc ctccccaaag aggaagaaac
     5041 gtcccattga ggagttgata atttcgggaa aggccgttag ccgacggagc gcttgaacgc
     5101 gcgggctccc ccatagagac acagcccgtc gaagacggcg agaacgacca gtgcgatggc
     5161 cacatgaggg agcgcttcgc tccagccggt aagaatcaat ggccgtatgg catcaatgac
     5221 ccaggtcatc ggattaaaga aagccagtga tttcatccaa cccggcatcg ccgctaatgg
     5281 gacgagtgcg gagctgagaa agatcatcgg gagcgagagg aagccaagca ctgagaaaaa
     5341 atctccgtga cttttcacgg aaaacgccat ggccatggaa atggcggtga gcccgacacc
     5401 aaatagcatg ccgatgacta aaatgagggc gattcctaaa atcccggttt ccgcactcac
     5461 gccaaacaaa aaggccactc ccagaataac cagtatttgc atggcggtga tggccatcac
     5521 aaagagaaac cgactgagga tgacggacga tcgtcggatg ggagttgcca tcaatcgctc
     5581 cagaaacccg ttttctttat caaacaggag gtctacaccc cctgccagcc cgttattgag
     5641 gacggtcatc acgacgacgc cggctgcaag gaaactcatg taattcgggg cttggacgac
     5701 ctgtacatcg gcagcacgtt gaaaaaggtt gccgaaaaag atgagccaaa acagcatggg
     5761 ctgaattagg gtaaacagca tactgaattt ttcccggctc aaacgcttga cccatcgatt
     5821 ggtcagggca agcacctctt gaatttgctc attcatggag aaacctttcc agaacagtga
     5881 gaatctatag gatatgattt taggtcgtgg gttcaggggg ttggtctgtg agggaccggc
     5941 ctgtgtgagc cacaaacacg tcgtctagtc gcggccgatg atattcgatt ccgtcgaggc
     6001 gacatcctac tcgattggtc gtctcgagga tagcaggcaa tgccttctcg ggagcatcga
     6061 cacggatatc aagaccctgt ggagtaggac ggatcgcacg cacgaagggt aaagctttca
     6121 gtgcggttga gagggtttca acgacctgag gcgcaggatc tccaagagtg agccagacac
     6181 catctccacc caggctggcc ttgagatcct ttggagagcc aatggcgcga atttctcccc
     6241 gatcaatgat ggcgattcga tcacaaagct gatcggcttc atccaggtaa ttggtggtca
     6301 tgacaatggt catgccctgc tcgcgcaatt gccggaggta ttgccaaata ttgaggcggc
     6361 tctgaacgtc aagcccaagc gtgggttcgt ccagaaaaag tactttggga ttcggcaaca
     6421 agccgcaggc gatatctaat tttcgtttca tcccgccgga atagccttta gccacgcgat
     6481 cggcatgttc ctccagatcg accagcttca aaagcgatgc aatccgggtg agggcttcat
     6541 tctttgggag atgatagaga tccgcgagca acagcagatg ttcccgtccg gtaagaaagc
     6601 ggtcgatggc ccgttcctga ggcacgtagc cgatcagccg tcgaatggta tcggcttctt
     6661 tgacgacatc atgtccgaag atcgttgcgg ttccggatgt ggggctcagg gtcgtaatta
     6721 aaattcgaag cgtggtgctt ttccctgccc cgttgggtcc caggaggcca aaaatttccc
     6781 cttgcgtgac ggacaaggag agatccttca ccgcggtcac ggtgtcgtag gttttcccca
     6841 gccctttaac ctcaatggca acggcatcag acatggtgtg ttatccttgt gccg
//
LOCUS       DAORAQ010000137         6854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1676_length_6854_cov_65.014855, whole genome shotgun sequence.
ACCESSION   DAORAQ010000137 DAORAQ010000000
VERSION     DAORAQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1676_length_6854_cov_65.014855"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..880)
                     /locus_tag="PKM72_10885"
     CDS             complement(314..880)
                     /locus_tag="PKM72_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNP61339.1"
                     /translation="MDLQIDSRNVTMTPRWKTEIEARMADLQVGHEDLIHGRVTLTKN
                     AHHKKSENVAEALVVVTMPGRHTLTARKEEKTFEEAIRAAFFAMEIEVKKFRDKRASK
                     EIRVPPIPLRGVITKLFPQEGYGFLLQDGGGEVYFHKNAVHGMKFEELEDGVEVLFNV
                     EDGEKGPQATTVNPLPILPGAVEKAAFS"
     gene            complement(992..1909)
                     /locus_tag="PKM72_10890"
     CDS             complement(992..1909)
                     /locus_tag="PKM72_10890"
                     /inference="COORDINATES: protein motif:HMM:NF039293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61340.1"
                     /translation="MSVVIRRFEPGTLQAMVQARTAHAIYRGAIHTIPTCFELVEQGG
                     VNFLIRIVSNLARKVQSNAEQRPPSRAVNIKQDPFLPYEQDLFVADISDTHVCLLNKF
                     NVLDHHILMVTRSFQEQDSFLTLSDIEAVLLCLAEFEGLAFYNAGEAAGASQRHKHVQ
                     MIPLPLTAEVSHLPIEPLLNAARFEEQIGQIPGLPFSHVLVRMNPEWIAVPAKGAQEL
                     LEQYLYMLRTVGLSGGDVGGETKSPGPYNFLITRQWMLLVPRSRECFEGISVNALGFA
                     GGLLVRDQSQLDRLKECGPMTVLRRVAMC"
     gene            2324..2743
                     /locus_tag="PKM72_10895"
     CDS             2324..2743
                     /locus_tag="PKM72_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP61341.1"
                     /translation="MATPNLQMKARDLMQTRMVAVTRQYSARDLSILIHSGTFSGVPV
                     IEPGNHLVGMVTEFDVLKALIDGKDLHTLKAEDVMTPNPVTVDEAATAEDIVNNMIKH
                     QIIRIPVVREGKLLGMISRTDLLNQLIDDHLINVYGA"
     gene            complement(2974..3516)
                     /locus_tag="PKM72_10900"
     CDS             complement(2974..3516)
                     /locus_tag="PKM72_10900"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNP61342.1"
                     /translation="MRALLVFVFILGCSATGWAKDIELVALGVRAGMNFKDAGLQPGE
                     KEDFEQFDVFGIMGLPWRWEGTSGWEVRSQVWGSAGALRGAGDLGFITTLTPGLALKI
                     PSWHLIIDGGVGGALISKWEFGRQDIGGPFQFIAHVGLGVQLPGNMVVGWRFHHMSDA
                     TMYGDSRGVDFHMLEVAYYF"
     gene            3873..4232
                     /locus_tag="PKM72_10905"
     CDS             3873..4232
                     /locus_tag="PKM72_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61343.1"
                     /translation="MLLIATIDPILRKNLQQALTKKGDPFLLLDEGENIIESVLRHNP
                     SLVVVDLYLVRPSGLEILRQLREKGFSGKVVVLGGQSNQSLTPEASHLGAVQIIGRPF
                     NANQVLGAVRVASGDLD"
     gene            4274..4555
                     /locus_tag="PKM72_10910"
     CDS             4274..4555
                     /locus_tag="PKM72_10910"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNP61344.1"
                     /translation="MKKYRNIGHHERMIRVGVGFLLLALSGFSLLPGWGDLVLLIVGL
                     IALLTGIIGYCPAWQALGINTCPLHKPEHAPSHTHPSAHEHPDPSSHRT"
     gene            4602..5519
                     /locus_tag="PKM72_10915"
     CDS             4602..5519
                     /locus_tag="PKM72_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61345.1"
                     /translation="MKTPTAPLKSKTILIPVDNSSQAEEAVRSVQAMSLPERVLLLHT
                     ISIPQLAYPGTGMSVGHHFSEAAEKALREEGVRILKKAASLLPPECKEVSQRLEIGIP
                     ASVILTLAQQESVDLIIMGSRGLGTIKEHLIGSVSHRVATHASCHVLLVKAPVVPFNS
                     ILLPIEQLLDAEWAVEFLSHKPFRGIPHITVLHVIPFAQPILPVGALLSDTWKKALQN
                     GGVHLTKNVTDKLSNLGYSVESLVQTGAPSSVIQRQISNLQPQLVLMGIPGHSPFQRL
                     THGSVSHATIHHSPCSVLLIREPSPQATA"
     gene            5596..6837
                     /gene="pfp"
                     /locus_tag="PKM72_10920"
     CDS             5596..6837
                     /gene="pfp"
                     /locus_tag="PKM72_10920"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNP61346.1"
                     /translation="MKFAILVGGGPAPGINSVIEAATIRSRLCGVEVLGIEDGFQWLM
                     KSDLSHTQPLTRETVSHLHFRGGSLLRTSRANPTKRPQDLSTTLTSLQKLNVTGLITI
                     GGDDTAFSALKLAELANGRLHVVHVPKTIDNDLCLPHGMPTFGFQTARHMGVELVKNL
                     MIDAHTTSRWYFVVTMGRKAGHLALGIGKAAGATLTLIPEEFKGATLKLGHLTNILVG
                     AMTKRLTQGRSDGVAVLAEGLSEFLDQDDLALLGGVERDEHGNIRLAELDIGKVLKET
                     VTQKLKHSEIKITIVFKNIGYELRCADPIPFDMEYTRDLGYCAAQFLLDGGNGAMVSI
                     VNGRFTPLPFEDIVDSTTGRTRVRMVDIESESYKIARAYMARLEAEDLANKENVSQYA
                     EILNMTPDQFRSTFATVANPT"
BASE COUNT         1799 a         1858 c         1530 g         1667 t
ORIGIN      
        1 agaactacca actatgtgca gaaagcccgc gtacatgtga tcacctgaac tgccgaaacc
       61 gtgcgttcag agatcaggat cctccacatc tccaggcagg gtcattttct ttaacaaaaa
      121 tttctctgca taatttgcgg gctgcccaac aagatcaggc cgagcatgca cggatatcat
      181 ccaatccttc aatgcgcaaa taactacaaa ccatttccat ggggcagaaa tttcggcacc
      241 tgctaaacac ccgggatctg ctcctcaggg gaaaccattc tgcgggaatc cctgcttctt
      301 aaatcaagcc tcgtcaggaa aacgcagctt tttcgacggc acccggtaga atgggaagcg
      361 gattgaccgt ggtggcctgg ggacccttct ccccgtcctc cacattaaac aagacctcca
      421 ccccgtcctc aagttcctca aatttcattc cgtgtaccgc attcttgtga aaatagacct
      481 cgcctcctcc gtcctggaga agaaagccgt acccttcctg gggaaataac ttcgtaatca
      541 ctccacgtaa cggaataggg gggacccgaa tttcttttga ggcccgtttg tcccgaaact
      601 tctttacttc aatctccatg gcaaagaacg ccgcacggat agcctcttca aacgtttttt
      661 cctcttttct ggctgtcagg gtatgacgac cgggcatcgt gacgactacc agggcttccg
      721 ccacattttc ggattttttg tgatgggcat tttttgtaag cgtcacccgc ccgtggatca
      781 aatcctcatg gcctacctgc agatcggcca tccgagcttc aatctccgtt ttccatcgtg
      841 gggtcatcgt cacatttcga ctgtcaatct gaagatccat agtgttttct tccctctcaa
      901 tttgagtaat agaacaacag acacacatcc tcaccacgta cacgttaagt atactcatag
      961 aaatgccaga ggaaaacatg ggaaaattcc cttaacacat ggccactcga cgaagtacag
     1021 tcattggccc acactctttc aaccgatcaa gctgcgactg atccctcacc aacaagcctc
     1081 cggcaaatcc caaggcattc acagaaatcc cttcaaagca ctccctggac cgtggaacca
     1141 ataacatcca ctgtcgtgtg ataagaaaat tatacggacc aggactcttg gtttcccctc
     1201 ctacgtcacc acccgacaat ccgacagtcc gcaacatata cagatattgc tccagcaact
     1261 cttgtgcccc ttttgccgga accgcgatcc attcgggatt catccggacc agcacatggg
     1321 aaaaaggaag accgggaatc tgcccaatct gctcctcaaa cctggctgca tttaataggg
     1381 gttcaatggg aagatgagac acttcagccg tcaaaggaag agggatcatt tgcacatgct
     1441 tatgccgttg actggccccg gcagcttctc ccgcgttata gaaggccaat ccttcgaatt
     1501 cggccagaca caacaacacg gcctcgatat cactcagcgt gaggaaggag tcctgttcct
     1561 gaaaagaacg ggtcaccatg agtatgtgat gatccaggac gttaaattta tttaacaaac
     1621 acacatgggt atcggaaata tccgccacaa acaggtcttg ttcgtaaggc agaaaggggt
     1681 cctgctttat gttgacagcc ctggagggtg gccgttgctc agcgttggac tgaaccttcc
     1741 tggccaggtt ggagacaatt cgaataagaa aattcacccc gccttgttcc accaactcga
     1801 agcatgtggg aatcgtatga atcgcccctc gataaatggc atgagcggtt ctggcctgca
     1861 ccattgcttg caaggtcccg ggttcgaatc gccgaatgac aaccgacatg attattccct
     1921 catctcaaga gaatgtcatc ccttaaaaag ctgacacctc gctcaaccgg gcatcatctt
     1981 gatagagtaa cctccgtttt tccaatgggt gaaggttttc aatcaggact tctctattct
     2041 gccagatttg accaactctt aacacagcag aaccatgaat tacatgatta ggtcagggat
     2101 tataggggtg gccctgcaga atgccttttt gtatagtcat cgtttgcatc cgatcccttc
     2161 caaaatggca gagaaaagat ggtgaagtaa agaaaagcga gaagaacctg gacaatcaac
     2221 tgccctatgc tttgctagaa ccactgtgcg agattgaata tacacgtcca cacgatccca
     2281 aacagagatt gcccccaaag ggaattttct atcaggaggt ttcatggcta caccgaatct
     2341 gcagatgaaa gcgcgtgatc tcatgcaaac ccgcatggtc gcggtgaccc gtcagtatag
     2401 tgctcgtgat ctctcgatcc tgatccattc cggcacgttt agcggagtgc ctgttattga
     2461 accaggcaat catttggtag gaatggtcac ggaatttgat gtcttgaaag ccctgatcga
     2521 cgggaaagat ttgcacacac taaaggccga agacgtcatg accccgaacc cggtgacagt
     2581 tgacgaagcc gcaacggcag aggacattgt caataatatg atcaagcatc agattatccg
     2641 gattcccgtc gtgcgtgaag gcaagctact gggaatgatc tcccgcactg acttattgaa
     2701 tcaattgatt gacgaccacc tgattaacgt ctacggagct taaccgggag ggtatttaat
     2761 tctgaatgcc ctcaagagac catatccccc aacagaggtt gcatagacgg tgcttctcat
     2821 tgggggcacc gtctttctcg cattctgatc cacctgtttc ttacaccccc aggaaaagac
     2881 ccgaaagcca gagaggacgt tatgaaagga tgggacagag agccatctct ccactcatgc
     2941 gaaaacagaa caatcctaaa gacatgaaga ccttcagaag tagtaggcca cctcaagcat
     3001 atggaagtcc acaccacgac tgtctccata catcgtggca tcagacatat ggtgaaatcg
     3061 ccagcccaca accatgttcc ctgggagctg aacacccagg cccacgtggg caataaattg
     3121 aaagggccca ccgatatctt gtcggccgaa ctcccacttg ctgataagtg ctcctccgac
     3181 tccgccatca ataattaaat gccaactcgg aatttttaaa gctaatccag gggtaagagt
     3241 tgtaataaaa ccaagatccc cggctcctct caacgcacca gcggaacccc agacttgcga
     3301 acgtacctcc cacccggacg tcccttccca ccgccatgga agtcccatga tcccaaaaac
     3361 atcaaattgt tcaaaatctt ccttttcgcc aggctgcaaa ccagcatcct taaaattcat
     3421 cccggctctc accccaagag cgactaattc aatatctttt gcccatccgg ttgccgaaca
     3481 tcccaggata aaaacgaaca ccaataacgc cctcattgag ttaccactcc ttttaaagaa
     3541 tttcagaatg caattaacac tgacttcatt tcacaccaac agccccccat accccagccg
     3601 gatgtccggc atgaataatc gtcattgaag aagaaggagt attacaaatc ctggaaaaat
     3661 taattgaaaa aattcatgag tgcaatgctc tcagattccc ggagtggggc caagcgctac
     3721 actcctcctc cactcaattg agattctttg ccccatttct tcatggctga aatctttgtt
     3781 cctgataaat caagggcatc tatctttgac attttggcac aggttttgat gggtatagat
     3841 tgtacaatat ttcaaatacg gatatctctt gtatgttact cattgcgacc atcgacccca
     3901 tcttacgaaa aaatttgcaa caggccttaa cgaaaaaagg cgacccattc cttttactgg
     3961 atgagggaga aaatattatc gaatccgtat tacggcataa cccctccctg gtcgtggttg
     4021 atttatatct ggtccgtccg agcggtctgg aaattttgcg tcaactccgc gaaaaaggat
     4081 tctcaggaaa ggtggtggta ttgggagggc aatctaacca aagcctcact ccggaagcca
     4141 gtcacctggg tgcagttcaa attatcggac gtcctttcaa tgccaatcag gtactgggcg
     4201 cagttcgggt tgccagtggc gacctagact aaatggcgca ataggttatc ttcaccatca
     4261 aaagaggtgt gatatgaaaa agtatcgtaa catcggacac catgaacgaa tgatacgggt
     4321 cggtgtaggg tttctccttc ttgccttatc aggattttca ctgttaccgg gttggggaga
     4381 tcttgtcctc ctgattgtcg gccttatcgc gttgttaacg ggaattattg gatactgtcc
     4441 ggcgtggcaa gctttgggaa tcaatacgtg cccacttcac aaaccggagc atgcaccttc
     4501 ccatacccat ccatcagctc acgaacaccc ggatccctcg tctcacagaa cgtaaagagg
     4561 caggtttcat tttgatggag tatcactaaa ttcgaggaca cgtgaagaca ccaaccgcgc
     4621 cattaaaatc caaaaccatt ttaattcctg tggataattc ctcacaggcg gaggaggccg
     4681 ttcgaagcgt acaggctatg tctcttccag aacgcgtact gctgctccac accatttcca
     4741 ttccccaact cgcctatcca ggaacaggca tgagtgtcgg gcaccatttt tccgaggcag
     4801 cagaaaaggc gttgcgggaa gaaggtgttc gaatcctcaa aaaggccgcc tcccttttac
     4861 caccggagtg caaggaggtc tcccaacgcc tcgaaattgg aattcccgcc tcggtcattc
     4921 taacattggc acagcaggaa tcggtggacc tcatcattat gggttcgcgt gggttaggaa
     4981 ccattaagga acacctgatc gggagcgtct cgcatagggt cgcgacccat gcatcttgtc
     5041 acgtacttct cgtcaaggct cccgttgtcc cgttcaacag catccttctt cccatcgaac
     5101 aacttctaga tgctgaatgg gccgttgagt tcttgtcaca caagcctttt cgaggcattc
     5161 ctcatattac ggtgctgcat gtcattccct ttgctcagcc tattctcccg gttggcgcac
     5221 tgttatcgga cacctggaag aaggcactcc aaaacggagg cgtccattta acgaaaaacg
     5281 tcacggacaa actctcaaac ctgggatatt ccgtagagag tcttgttcaa acaggggctc
     5341 cttcctcagt cattcaaaga cagatctcaa atctgcaacc ccaattagtc ctcatgggta
     5401 tccccggtca ttcacccttt caacgactga cacacgggag tgtgtctcat gcgaccattc
     5461 accattctcc ctgttccgtg ctcttgattc gtgagccctc tccacaagct accgcttgat
     5521 tactcatacg ccctctttct attatggtcc ccattaataa ttcacgtgag aaacgacatt
     5581 atggattccc catctatgaa attcgccatt ttggtcggag gcggaccggc ccccggcatc
     5641 aatagcgtca ttgaagccgc cacgattcga agtcgtcttt gtggggtgga ggtgcttggg
     5701 attgaagatg gatttcagtg gttgatgaaa agcgaccttt cccatactca acctctcacc
     5761 cgtgaaacgg tcagccactt gcactttcgt ggtggctcct tattgcgcac atctcgggcc
     5821 aatcccacta aacgtcccca agatttatca accacgttaa cctcccttca aaaattgaat
     5881 gtgaccggcc tcatcaccat tggcggggat gatacggcct tttccgcatt aaaactggcg
     5941 gaactggcca atgggcgatt gcatgttgtt cacgtgccca aaacgattga taacgattta
     6001 tgccttcccc atggcatgcc gaccttcggg tttcaaacgg cccggcacat gggagtcgag
     6061 ctggtcaaaa atctcatgat tgacgcccat accacctccc gatggtattt cgtggtcacc
     6121 atgggacgta aggccggaca cctggctctc ggcattggta aggcggcggg agcgaccctt
     6181 accctgattc ctgaggaatt caagggggcc acgctcaagc ttggccactt aaccaatatt
     6241 ctggttggag ccatgaccaa acgtctaacc cagggccgtt cggatggtgt ggcggtgttg
     6301 gctgaaggac tttcggagtt tttggatcag gacgacctcg ccctcctagg cggggttgaa
     6361 cgagatgaac atggcaatat ccgactggcg gaactggata tcggaaaggt cctgaaggaa
     6421 accgtcactc aaaagctgaa acactctgag atcaagatta ccatagtatt caaaaacatc
     6481 ggctatgaac ttcgctgcgc cgaccccatt ccgttcgaca tggaatacac ccgggatttg
     6541 gggtattgtg ccgcccaatt ccttttggat ggagggaatg gggccatggt gtccatcgtg
     6601 aacggacgct tcaccccgct gccttttgaa gacattgtgg actccactac gggccgtacc
     6661 cgagtgcgaa tggtcgatat tgaatcagaa tcttacaaaa ttgcccgtgc ctatatggcc
     6721 cgattagaag cggaagattt ggcaaacaag gagaacgttt cgcagtatgc cgaaattttg
     6781 aatatgactc ccgaccaatt ccgtagtacc tttgccacgg tcgctaatcc aacctgaata
     6841 ttcacgatat gttc
//
LOCUS       DAORAQ010000138         6759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1699_length_6759_cov_70.212112, whole genome shotgun sequence.
ACCESSION   DAORAQ010000138 DAORAQ010000000
VERSION     DAORAQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1699_length_6759_cov_70.212112"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKM72_10925"
     CDS             <1..542
                     /locus_tag="PKM72_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61347.1"
                     /translation="VGLDLYLQMVEQAVQQLKGQEVEEEWEPTLQLDVSAFIPEDYVE
                     DASQRLGLYKRLSGSERLGDLALLYGETLDRFGSPPTAMERLFEVMQIKVLAKALQLE
                     YVEVRGGLMNVRFHERAKLPEQGIRALMDSWKDRLDCLSPRAYRLTLPEQDWASMYPR
                     ANTILQELYQSMAKEEVKR"
     gene            544..1464
                     /locus_tag="PKM72_10930"
     CDS             544..1464
                     /locus_tag="PKM72_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNP61348.1"
                     /translation="MKGVRECLKGCSSHTFLIWVVACLLFFPGVGLSLTDRIIAVVNK
                     EVITWSDLDKEIRDEYKRLTAKYHGEELDRRYHQKQREVLNRLIDDRLQIQEAQARGV
                     TVTQDEIDAALQRTPLPPTQTEEEFAHQMLLKKLYDFEVRRNVVVEEEEIKRFYDANP
                     TLFLTSPHYRLKQCLIAGKEGFERERARKKAQTMYAAWTPTTSLEDLATSHFEQVGEL
                     GWVRENELLAPLKQVVKELKPGHISAPIETLLGFHLVLVEEIQQPQPLPLEAVERDIR
                     GMLTKQRSEEAYRNWLADIKQKAFIDVRLK"
     gene            complement(1531..2784)
                     /locus_tag="PKM72_10935"
     CDS             complement(1531..2784)
                     /locus_tag="PKM72_10935"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNP61349.1"
                     /translation="MESKITYQKIASHEAKKGTFDKCVLLYSGGLDTSIMLKWIQEAY
                     ACSIVALTVDVGQLHEDLEAVKAKAIKLGAEEAVVIDAREEFAETLLSRAIKANADYQ
                     GGYPLSTPLARVTLSEIAVRVAKERGIKVIAHGSTGKGNDQVRFENYITTLDATMKVI
                     APVREWSMGRDEQIDYAKQHDIPIKQTKEYLYSHDDNMWGSTNEGGDIEDPACIPDLR
                     KFLQVCIPPEQAPDEPEIVEVGFNKGIPCSINGEELSLCRVIERANAVGARHGVGIVH
                     LTEDRLVGLKVRGVYEAPGAEILIKAHFNLEKLVNTRDLNELKKFIDEKWAYTCYGAK
                     WFDPSMDAIHAFQDSANQRVKGTVKVKLFKGKADVVAMKSPYSLFDANLATFNKDASF
                     NQNASAGFIEIYNLAQKTYRRFDQV"
     gene            complement(2899..4077)
                     /locus_tag="PKM72_10940"
     CDS             complement(2899..4077)
                     /locus_tag="PKM72_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNP61350.1"
                     /translation="MVSGAVAGGVLGALAPEVARHVEILGELWIRMLRMLVVPLIVAS
                     IIQGVGSLGDIRQLGKLGGVTVTYYLITSGMAIGLGMILVSLIKPGAGADIAAAMAPS
                     GVEAAVNIGWLDLIKGIFSPNLFAAASQGELLPLVIFSLAFGAALTTVGENGTLVLKF
                     FEGVFGAIMKLVHLVMWIGPIGVFGLVAGRLANAGGIDGIVALLVGLGLFTITVLLGL
                     AIHAGIILGFILHFGGQRNPLTYVRALGAALTSAFATASSSATLPLTMEGVESAGVSS
                     RSSRFVLPVGATVNMDGSALYEAVAAVFIAQAWGIELSGGALIVLFVVATVSAIGAAG
                     IPEAGLVTMVVVFQAVGLPLEGLGLLLAIDWLIDRFRTAVNVWGDAVGAAVVDRYVTV
                     "
     gene            complement(4169..4441)
                     /locus_tag="PKM72_10945"
     CDS             complement(4169..4441)
                     /locus_tag="PKM72_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61351.1"
                     /translation="MSFVQVRQSTLTGERKTFISASAIAYVQELKPDQDLGWQIGVEP
                     SRALIYLHQKLEPIISLDSFDDIVRQTKTTTLGVGVAGQKLAKKTK"
     gene            complement(4475..6469)
                     /locus_tag="PKM72_10950"
     CDS             complement(4475..6469)
                     /locus_tag="PKM72_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61352.1"
                     /translation="MSDSPQGSSQPELMDATARQEQALLQALEALEQAEPFAKAKYQP
                     EVIRRATDLFESDEGLQIVRGQAHRFDSAGVFHAGPWEHPDRLLAELVGGGLRAEGQY
                     SSLEMLSELRVLSIAAGDSQHPKFSAQLAQAFLQTVMGLNLDLLYGSETEESRLRPKV
                     YARARRILAMIEQEVSSEGLLEHVLDDIDARVAQRPIDVSLTLKMIEQAKNIQGDPDP
                     KLAARLDRYLKATGAPTPLAQKAGSPTNYRNLLAKATTHEIENEAKVVGKLLTLTGLS
                     SEYHVAFLQHVLKNDETGILAIALDLTEVGQAHMEQYREKVFELMRLTIHPATSWIIY
                     GFQQMLERMLLARPEVADGLTKLLNLDLCSTAQQVTKKHLPRGTGITANAAIVGGAIA
                     MLGQPLGVGQGNNPTCQGARALSLWSLHDPGYLLQLLTSAARDDTVEFLFEGQRILSG
                     EIGGGVAEGKFDLKLDPVSRILVPHLDRIYDEKMRRAALRGEDPHKWVNPALYGRWVA
                     TSLASITMVNQTVSGYEEFLRLFYATHHPLYDGGHDLVYPNPVGLLITNVHGVFLGYH
                     AVSIQRVAEDEEGKVRVYFFNPNNEGRQNWGRGVEPSVVGHWEIPGESSLTFEHFAAH
                     IYAFHYNQMEVGDPKAVPAEIIAEVTAHAKEGWGQAFTWL"
     gene            complement(6462..>6759)
                     /locus_tag="PKM72_10955"
     CDS             complement(6462..>6759)
                     /locus_tag="PKM72_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61353.1"
                     /translation="NTSKTELDRFFPKEKDHKVFAANLMVYPRKASVHQLQFPKELEA
                     HPYMDRHTMFEPVTAKVAERVGFGNHYGTVFLAGEDPEALRELVQRYEEYDFYE"
BASE COUNT         1758 a         1889 c         1553 g         1559 t
ORIGIN      
        1 ccgtcgggtt ggacctgtac ttacaaatgg tggagcaggc tgttcagcag cttaagggcc
       61 aggaagtgga ggaagaatgg gaacccactc ttcaacttga tgtctcagcc ttcattcccg
      121 aagactatgt ggaagatgcc tctcagcggc ttggactcta taaacgctta tccgggagtg
      181 aacggttagg ggatttggcc ttattatatg gggagacgct cgaccgcttt ggatcgccac
      241 ctaccgccat ggaacgccta tttgaggtca tgcaaatcaa agtattagcc aaggcccttc
      301 aactcgagta cgtcgaagtc cgtggcggac tcatgaacgt gcgctttcat gaacgggcca
      361 aacttccgga acagggaatt cgtgctttga tggacagctg gaaagatcgt ctggactgtc
      421 tttctcctcg tgcctatcgc ttgactcttc ccgaacagga ttgggcctcc atgtacccca
      481 gggccaatac gatattacaa gaactctatc agtcgatggc caaagaggaa gtgaaacgct
      541 gacatgaagg gcgtgcgcga atgcctgaaa ggctgctctt cacacacgtt tctcatctgg
      601 gtagtggcat gccttctctt ctttcccggc gtaggcctga gtctgaccga ccggattatt
      661 gcggtggtga ataaagaggt gattacctgg tctgatctgg ataaggaaat acgggacgag
      721 tacaagcgct tgaccgctaa atatcacgga gaggaactgg accgccgata ccaccaaaaa
      781 caacgagaag tcctaaatag actcatcgat gatcggctcc aaattcaaga agcccaagcc
      841 aggggggtca ccgtcaccca ggacgagatt gatgccgctt tacagcgaac ccctctgcca
      901 cctacccaaa ccgaagaaga attcgcccat caaatgctcc tgaaaaaact ctatgacttc
      961 gaggttcgcc gaaacgtcgt tgtcgaggag gaggaaatta aacggttcta tgacgccaac
     1021 ccaactctat ttctcacatc cccacactac cgactcaagc aatgcctgat agccggaaaa
     1081 gaaggatttg aacgcgagcg agcaagaaag aaagcccaaa ccatgtatgc cgcatggact
     1141 ccgactacct cgttagagga cctcgctacc agtcatttcg agcaagtggg tgaattggga
     1201 tgggtgcggg aaaacgagtt attggcccct ctgaagcagg tagtcaagga actgaaaccg
     1261 ggacacatct cggcccccat tgaaaccctc ttaggatttc acctcgtcct ggtggaagaa
     1321 attcagcaac cccaaccgct cccccttgaa gccgtggaac gagatatccg tggaatgctg
     1381 accaagcagc gatccgagga agcgtatcgg aattggctgg ccgatataaa acaaaaagcc
     1441 ttcatcgatg tgaggcttaa atagagaggt tcatttcacc cctgctggcc caagaccttc
     1501 ttccatcacg aatccctctc attctccatc ctacacctgg tcaaagcgcc gataggtctt
     1561 ttgggccaga ttgtaaatct caataaaccc tgcagacgca ttctggttga aactcgcatc
     1621 cttattaaac gtggccaaat tggcatcaaa taaagaatag ggagatttca ttgcgaccac
     1681 atcggctttt cctttaaaca gctttacttt caccgttccc ttcacacgct ggttggcgga
     1741 atcctggaaa gcatggatgg catccattga cggatcaaac catttggccc cataacaggt
     1801 gtacgcccac ttttcatcaa tgaatttttt cagttcgttt aagtcacggg tattgacaag
     1861 tttttccaga ttaaaatgcg ctttgatgag gatttccgct ccaggagctt catagacgcc
     1921 acgaaccttc aatccgacta aacggtcctc cgttaaatgc acaatgccta ccccatgcct
     1981 tgcccctaca gcattggcgc gttcaatgac ccggcaaagg gaaagctctt caccattgat
     2041 tgaacaaggg attcctttgt tgaacccaac ttcgacaatt tccggttcat ccggtgcctg
     2101 ttccggggga atgcacacct gcagaaattt tcgtaaatca ggaatgcagg ccgggtcctc
     2161 aatatcccct ccctcattag tcgagcccca catattatca tcatgcgaat acaggtactc
     2221 tttagtttgt ttaatgggga tatcgtgttg tttggcataa tcaatctgtt catcccggcc
     2281 catactccat tcacgaaccg gagcaatcac tttcatcgtg gcatccaatg tcgtgatgta
     2341 attttcaaag cgaacctggt cattcccctt tcccgttgag ccgtgagcaa tgactttgat
     2401 tccccgctcc ttggccacgc gaacggcaat ttccgaaagc gttacccggg ctaggggagt
     2461 cgagagagga tatcccccct ggtaatccgc attggcttta atggcccgag acagcagtgt
     2521 ttcagcaaat tcttccctgg cgtcaatgac caccgcctcc tcagcaccca gtttgatcgc
     2581 cttcgctttg accgcttcaa gatcctcatg taattggcct acatcgactg tcagtgccac
     2641 aatggaacag gcataggctt cctgaatcca tttaagcata atggaggtat ccagtcctcc
     2701 cgagtacagt aaaacacact tatcaaacgt tccctttttg gcttcgtgag atgcaatttt
     2761 ctgatacgtg atttttgatt ccatgccgtg ttcacctaaa ataaggttgt gtacatttat
     2821 tgagccctca ccatgtgtca accgcaagag ctcgcttttt tattgaataa taacccaaga
     2881 agatgaagga attcgatgct agacggtgac atagcgatcg accacggcgg cacctaccgc
     2941 atcaccccag acattcactg cggttcgaaa ccgatcgatc aaccagtcaa tggccaagag
     3001 gagtccgagc ccctcgagtg gaagcccgac agcttgaaag accacaacca tcgttacaag
     3061 cccagcttca ggaatccctg cagcgccaat ggcagacacg gtcgccacca caaacaggac
     3121 aataagcgcc cctccactta attcaatccc ccaggcttgc gcgataaaca ctgcggctac
     3181 ggcttcatac aacgcggatc catccatatt gaccgtcgca ccgactggaa gaacaaaccg
     3241 acttgatcga ctgctcaccc cggcactttc gaccccctcc attgtcagag gcaaggtcgc
     3301 agacgaactc gcggtcgcaa acgcactcgt cagagctgcg ccaagagcac gcacatatgt
     3361 caaaggattc cgttgacctc caaaatgcaa gatgaacccc agaataattc ctgcatggat
     3421 ggccaatccc agtagcacgg taatcgtgaa taaccctaaa ccaactaaaa gcgcgacaat
     3481 accgtcaatt ccaccggcat ttgctaagcg ccctgccacc aagccaaaca cgccaatggg
     3541 accgatccac atcacaaggt gaaccagctt catgatcgcc ccaaacaccc cttcgaaaaa
     3601 cttgagcact aatgtaccat tttccccgac cgtcgtcagg gccgccccga atgccaaaga
     3661 aaagatgacc aatggcaata attcaccctg cgaagctgcc gcgaataaat tcggggaaaa
     3721 gattcccttt atgaggtcga gccatccgat gttcaccgcc gcctcaactc cggaaggggc
     3781 catcgccgcg gcaatatcgg ctcccgctcc aggtttaatg agactgacca ggatcattcc
     3841 cagcccaatg gccataccgg aagtaattaa atagtaggtg accgtcactc ctcctaattt
     3901 tcccaactgg cgaatatcac ccagggatcc aacgccctgg attatcgaag ccacaatcaa
     3961 gggcaccacc agcatccgca acattcggat ccagagctca cccagaattt ccacatggcg
     4021 agcgacttct ggagccaggg ctccgagcac cccgccagcc accgcaccgg acaccatgag
     4081 ccacacgagt ggaccatggc catttccacg atctacagat aagggttgtt tggtaatggg
     4141 acaaacctga ttttagggaa gggaaacgct acttggtctt tttggctaat ttctgcccag
     4201 ctaccccgac acccagagta gtggtctttg tttgcctcac aatgtcatca aaactatcga
     4261 gagaaataat gggctcaagc ttctgatgca agtaaatgag tgccctggat ggttccacac
     4321 caatctgcca accaagatcc tggtcaggct tcagttcttg aacataggca atggccgatg
     4381 cactgataaa ggtctttcgt tcccctgtga gggttgattg cctgacctgt acaaaagaca
     4441 tgctcacccc tttcaatcgt aaccgttcaa aggtctacaa ccaggtgaag gcctgccccc
     4501 acccttcctt ggcatgtgcg gtcacctcag cgatgatttc ggccggaacg gcttttgggt
     4561 cacccacctc catctgatta taatgaaacg cataaatatg agcggcaaaa tgctcgaagg
     4621 ttaatgacga ttctcccgga atttcccaat gaccgaccac agacggttca acccctctcc
     4681 cccaattttg acggccctca ttattcggat tgaagaaata gacgcgaacc ttcccttctt
     4741 catcctcggc aacacgctgg atggagacgg cgtgataacc taaaaagacc ccatgcacat
     4801 tggtaataag gagaccaact ggattcggat acaccaaatc atgccccccg tcgtataaag
     4861 gatgatgggt ggcataaaat aaccgtaaaa attcctcata cccggagacc gtctggttaa
     4921 ccatagtaat tgaagccaga ctggtcgcca cccaccgacc atacaaagcc gggttcaccc
     4981 atttgtgtgg atcttcaccc cgcaacgccg cacgcctcat cttttcatcg tagatacgat
     5041 ccagatgcgg taccagaatg cgtgataccg gatcgagctt caaatcaaat tttccttccg
     5101 cgactcctcc cccgatttct ccggataaga tccgctgtcc ctcaaaaaga aattcaacag
     5161 tgtcgtcccg agccgcgctg gtcaataatt ggagcaaata cccaggatca tggagactcc
     5221 agagactcag ggcacgggcg ccctgacagg tcgggttgtt tccctggccg actcccaacg
     5281 gctgccctaa catggcaatc gccccaccca caatggcagc attggccgta atcccagtcc
     5341 cgcggggcag atgtttcttg gtgacttgtt gggcagtaga acacaaatca agattaagca
     5401 atttagtcag accatcagcg acctctggtc gtgcaagaag catccgctct aacatctgtt
     5461 gaaacccata gatgatccaa ctcgtagcag gatgaatggt caggcgcatt aactcaaaga
     5521 ccttttcccg atactgttcc atgtgggcct ggcctacctc ggtcaaatcc aaggcaatcg
     5581 ccaggattcc cgtttcgtca ttcttgagga catgctggag gaacgcgaca tggtattcgc
     5641 tggatagacc ggtcagcgtc aacaatttcc ccaccacctt ggcctcattc tcaatctcat
     5701 gtgtggtggc tttggccaaa agattgcgat aattcgtggg agagcccgct ttctgggcca
     5761 gtggtgtggg agcgcccgtg gccttgaggt accggtccag tctagcagcc agtttggggt
     5821 ctggatcccc ctgaatgttt ttggcttgct caatcatttt taatgtgagg cttacatcaa
     5881 taggacgctg agccactcgc gcatcaatat catcaagaac atgttccagc aacccttccg
     5941 aggaaacctc ttgctcaatc atggctaata ttctgcgggc tcgtgcatag accttcggac
     6001 gcagacggga ctcctcggtc tcactgccgt acagcaaatc aagattgagc cccataaccg
     6061 tctgtaaaaa tgcttgagca agttgcgcag aaaatttcgg gtgttgcgag tcgccggctg
     6121 caatggataa aacccgaagc tcgctcagca tttctaatga cgaatactgc ccttcggcac
     6181 ggagcccccc acccaccaac tcggctagaa gccggtcagg atgttcccag ggtccggcat
     6241 gaaagacccc tgcggaatca aaacgatggg cctgtcctct tacgatttgc agaccttcgt
     6301 cgctttcaaa caaatccgtg gcacgacgaa taacctctgg ctgatacttg gccttggcaa
     6361 atggttccgc ctgctccaaa gcctcaaggg cctgaagtaa cgcttgttct tgacgggcag
     6421 tggcgtccat taattcaggc tgagaagagc cttgggggga atcactcata gaaatcatac
     6481 tcctcataac gttggacgag ctcacgcagg gcttccggat cttctcccgc cagaaagacg
     6541 gtcccataat gattaccgaa acccacacgc tctgccactt tggcggtgac aggttcaaac
     6601 atggtatgcc ggtccatata gggatgggct tccaattctt ttggaaactg tagttgatgg
     6661 acgctggctt ttcgaggata caccattaaa ttagctgcaa aaactttatg gtctttttct
     6721 tttgggaaaa accggtccaa ttctgttttg gaggtattg
//
LOCUS       DAORAQ010000139         6696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1716_length_6696_cov_66.208553, whole genome shotgun sequence.
ACCESSION   DAORAQ010000139 DAORAQ010000000
VERSION     DAORAQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_6696_cov_66.208553"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="PKM72_10960"
     CDS             <1..1862
                     /locus_tag="PKM72_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNP61354.1"
                     /translation="DTLEHLRMLSNLETPKDKLIQIVLLGQPELDGLLDRYELRQLRQ
                     RIAVRAIIHPLSKRESFEYIQHRLDKAGGEGKSIFTNTALALIVQEAKGIPRRLNIIC
                     DNALVTAFGYNKALVTAKIAKEVIDDLTGRPSHSLWNLVPLTGLALILVLALFALLPL
                     TESKFSDIPTIYETGQLDKERENPKQILVPEQDLVKGGKIIPSPGQTGPTLTERAQTL
                     LTESVSEVFENLRNRVDSHSESTTTPPEERTNTNPDNHEDLLLGFSPDNFQEKVPDAT
                     EELEVAAVTPETVSPGILLPQDTIFDLPSQEESIAKTEADSQEKGARFIRDETQFIPS
                     AQQALSELLVQEEPIATAAEANRMVEAATLIQDEAETPSEKASENSLVHLRPEPLSER
                     MGKPTIVAQEPIRLPSQDSLTSVPIAQTPMTSQEITNKQSEAEASPSPVTRIVKRGDT
                     LAKLLQSIYGTASPSHVQFVLEHNPHIVSARKMYPGQKIMFPPLENIESNNTVAEAKK
                     SLGAGKDTVLPMSTKIFAHSSSQIKPSEKKVEVNRDLPYAVATVQKGDTLEKLVKVVY
                     GSSHPSYVQRVLEYNPKIRNPKEIFPGQDITFPKIAGEMKTRTTQASQSNSNE"
     gene            2092..3552
                     /locus_tag="PKM72_10965"
     CDS             2092..3552
                     /locus_tag="PKM72_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNP61355.1"
                     /translation="MTPQKTATQFISIRDILTLFFKHKNKFLAIFGGTVAIAIGTAFL
                     MTPIYQAQSSVMVKMGRENVYHPEIGAMNPEISMDQKSMIESEVQILLTRDLTKRVLE
                     VLKVGVVYPKITENAPENISSIDAGISEMRKNLSVSKVSESNIIEISFLHQQPEIAEK
                     VVNLLVDLLLEKHLEIFSNPKGLFLSNQLHDYEQKLHKSKSQLQAFKQQNKLTHLDEE
                     KALLLGQRNELNTIHKNLQNEEKGLKSKYESLKGQIKNVPKHVALTSVSEQQKVIDGA
                     QANLLQLGLEEQRLLAKYHETSRFVVNIRNEIRLVEQFIKNQEGSLNDKVTKGINPVY
                     QGLEVEILTVESEINAHVSQINEVTFQLKKLYEQLAKLDFLEKEFETLQMKVDSDKNN
                     YKMYLTKVEEAKVSEAMDQLKMANISVIQPATLPLRPVKPQKGLILFLGIIIGGISAI
                     LWVIATEYLESGYSRPEYASQDLGLPILTSIPYKNL"
     gene            3724..>4493
                     /locus_tag="PKM72_10970"
     CDS             3724..>4493
                     /locus_tag="PKM72_10970"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNP61356.1"
                     /translation="MSKVFEALQYAYDKRELEKKEQRSDFPSVISTYTPSYTPSFTSP
                     LPALEMQDEMARMRQSISSLLPPPGRNIIQFISSGKGEGTSLIVREFGMLLAKQNNKS
                     VLLVDTCPGHADQHQAFHVEPKIPLESTIRHGGSLDLSISRIPHPQQSLSLCFLSENH
                     DSNSSTSWLSRDMVPWEPVQKQFDYILIDSPPISSSHDGLALCSLVDGVVLVMEAVHS
                     RSHVVQSSRDKLIESGGNILGIAFNKQKRYIPDWIYRRL"
     assembly_gap    4494..4593
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4594..6203
                     /locus_tag="PKM72_10975"
     CDS             <4594..6203
                     /locus_tag="PKM72_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNP61357.1"
                     /translation="FYAMGFFCTFLIPLQIFAAIGAPNTLQATQSDRVIHVNNFPPHA
                     SDSNPGTKALPLKTIGMATTLAVENHKRGFSTKILIYPGVYREKIHMEFSSRKLDPTI
                     TFEAIEQGKTIISGSEIWENWEGTKLKNIYTHKWPFKWGVRPYPPGWEGKTILQPIVR
                     RQEMVFVNGIKFNQVDLHSEMKPGDFSVSEENSEVRIFLPDNVDIKQSQVEIAVQKGL
                     FTIKGKSNFVLKGLRFQHDASGLVGHALGITNAKNVYLEDCVFAWNNWGGYQFYNVQN
                     VTTRRNVSTHNGGPGHTAWRIKNYLSEDDETSFNNWRGAKGNFVYWAVAGTKIMGIHN
                     ATFRRHRAQGNHTAGFWLDYDNKNIEIEDGVSCGNLKHGLFLEATQGPLTITNMKIYQ
                     NHEWGIRIVNSSQINVTESQVYHNRGPQIRVQGQKTRGVNDWETGERLNLGAEKWTLE
                     NNIIVGDGLLVQSSFKNPLGPGLKSNGNLWSSASNTKPFQAAADRLTFSEWQISMNQD
                     QTSTFAFPVQPESGQALLDNCTYRGLD"
BASE COUNT         2094 a         1468 c         1363 g         1671 t
ORIGIN      
        1 tggacacatt agaacatctg cggatgttgt caaatttgga aacgccaaaa gacaagctca
       61 ttcaaattgt gctgctgggt caaccggaat tagatggcct tctggatcgt tatgaattaa
      121 gacaattacg gcaacgcatc gccgttcgag ccatcattca ccccctctcc aagcgcgaaa
      181 gttttgaata tattcaacat cgcttggaca aggccggagg ggaagggaaa agtattttca
      241 cgaatacggc cttagcgtta atcgttcagg aagccaaggg gattccccgg cgcctgaata
      301 ttatttgtga taacgccctg gtcacggcgt ttggctacaa taaagccctg gtgacagcaa
      361 aaattgccaa agaagtgatc gatgacctga ccggccgccc ctcccactca ctctggaact
      421 tagtaccttt gacaggcttg gctctcatcc ttgtcctggc actttttgca ctcctacccc
      481 tgactgaatc caaattttcc gatatcccca caatatatga gactgggcaa ctggataagg
      541 aaagggagaa tccgaaacaa atcctcgtac ctgaacaaga cctcgtaaag ggagggaaaa
      601 ttatcccttc ccctggtcaa acaggtccaa ctttgaccga gagggctcaa acgctcctca
      661 cggaatcagt atctgaagtc tttgagaatt tacggaatcg ggtcgactct cattccgaat
      721 caaccacgac tccacccgaa gaaagaacga ataccaatcc cgataatcat gaagacctcc
      781 tgctggggtt ttcacccgat aatttccagg agaaggtacc tgatgctaca gaagaattgg
      841 aggtagctgc tgtaactcca gagacggtat caccaggcat cctgctcccc caagacacaa
      901 tcttcgatct cccaagccag gaggaaagca ttgcgaagac tgaagcagac agtcaggaaa
      961 aaggagcgcg gtttattcgg gatgaaacac aatttatccc gtccgcccaa caggcactct
     1021 ctgagctctt agtccaggaa gaacctattg cgacggctgc tgaggcaaac cgtatggtag
     1081 aagcagcgac gctgattcag gatgaggcag aaacaccatc tgagaaggcc agtgagaaca
     1141 gccttgttca cctgaggccg gaacccctca gtgaacgtat gggaaaacct acgatagtcg
     1201 ctcaagaacc aatccgcctc cctagccaag atagccttac ttctgtccct atcgctcaaa
     1261 cacctatgac atcacaggaa ataacgaaca aacaatccga ggcggaagca tcgccttcac
     1321 ccgttaccag aatagtgaaa aggggtgaca ccctggcaaa gttattgcag agtatttatg
     1381 gtacagctag tccttctcac gttcaattcg ttcttgaaca taaccctcat attgtgagcg
     1441 cgcgaaaaat gtacccaggg caaaaaatta tgtttcctcc tttggaaaac atcgagagca
     1501 acaatacggt tgctgaggct aaaaagtctt tgggggcagg taaagatacg gtattaccca
     1561 tgtctacaaa aatctttgct cactcttcct cacaaattaa accaagtgag aaaaaggttg
     1621 aggtcaaccg agatcttcct tacgcggtgg ccaccgtaca aaaaggcgat actttagaaa
     1681 aattggtcaa ggtcgtatat ggatcctcgc atcctagcta tgttcaacgt gtcctggaat
     1741 acaaccccaa aattcggaat cccaaggaaa tctttccagg gcaggacatt acctttccta
     1801 aaatagctgg agaaatgaaa acccggacaa cccaagcgtc tcaatccaat tcaaacgaat
     1861 agattgaacc ctatgattgc aagggcaccg gatggcccag caattgtttg gagccggaaa
     1921 agtctcgatt gaacaaagcg gagcaaggcc gttcctggat ggagtttccc acaagtatat
     1981 gcaacatttt ttcctttgag gttttgccca aaagtcgcta atccatactt accaaagtac
     2041 cagagaaatt cataaatcct gaaatatctt tcaagggata atttacgtcg gatgacacct
     2101 caaaaaacag ccacccaatt tattagcatc agagatatcc ttactctgtt ttttaaacac
     2161 aaaaacaaat ttcttgccat atttggtggt actgtcgcca tagctattgg tacagctttt
     2221 ctcatgacac caatttacca agcgcaatca tctgtcatgg taaagatggg cagagaaaat
     2281 gtttatcatc ccgaaatagg cgccatgaat ccggaaatat caatggacca gaaaagcatg
     2341 attgagtcag aggtccaaat attacttacc agagatctta cgaaacgtgt actcgaagtc
     2401 ttaaaggtag gagtggtcta tccaaaaatc actgaaaacg ctcctgaaaa catttcttcc
     2461 atagatgccg gcattagcga aatgcggaaa aacctctccg tatctaaagt tagtgagtca
     2521 aatattattg aaatcagctt tcttcaccaa caaccggaga ttgctgaaaa agtggtcaac
     2581 cttcttgttg atctcctttt ggaaaagcat cttgaaatct tcagtaatcc gaaagggttg
     2641 tttttgagca accagttaca tgactacgaa caaaaactgc acaaatccaa gtcgcaactc
     2701 caagcattca aacaacagaa taaacttacc catttggacg aagaaaaagc gctcctgctg
     2761 ggacagcgaa atgaattaaa cacaattcac aaaaacctac aaaacgaaga aaaagggtta
     2821 aaaagtaaat atgaatcatt aaaaggacag ataaaaaatg tccccaagca tgtggccctc
     2881 acgagtgtct ctgagcaaca aaaggtcatt gacggagccc aagccaactt acttcaattg
     2941 ggcttagagg aacagcgtct attagccaaa taccatgaaa caagccgttt tgtggttaat
     3001 attaggaacg agattagact ggtcgaacag ttcataaaaa atcaagaggg ctctctaaac
     3061 gataaagtca cgaagggaat caacccggtt tatcagggat tggaagttga aattcttaca
     3121 gtagagtcag aaatcaatgc tcatgtctcc cagatcaacg aggttacgtt tcaactcaaa
     3181 aaattatatg aacagttagc caaattggat tttttagaga aagaatttga gaccctccaa
     3241 atgaaagttg attcagataa aaataattat aagatgtatt taacgaaagt tgaggaagca
     3301 aaagtatcgg aggcaatgga ccaacttaaa atggccaata tcagtgtgat tcagccggcg
     3361 actttgcccc tgagacctgt caaaccccaa aaagggttaa ttctttttct cggaataatt
     3421 attgggggta tctcagcaat cctctgggtc attgcgaccg aatatttaga aagcggttat
     3481 tctcgacccg aatatgcttc acaggattta gggttaccaa ttctaacaag cattccctac
     3541 aagaacttat gattgtatgc aggcacaaaa aaatcccaaa gatcaaatgg acgaatgtta
     3601 ccaacaacaa acgcgacgaa tgacaaacca ccccatagct ttaaattgaa tcgaaaaggc
     3661 gacaagatgt tcagcaataa ggaaagtcga tgtaaccgaa tggatcaaga agggtttaga
     3721 tgaatgagca aagtttttga agctttacaa tatgcatatg ataagcgtga attggaaaag
     3781 aaggaacaaa gatccgattt cccttctgtc atttctacct atacgccatc ctatacgccc
     3841 tcctttacct cccccttgcc agcactggaa atgcaggatg aaatggcgcg tatgcgccaa
     3901 agtatttctt cacttcttcc tccgccaggg agaaatatca ttcaatttat tagttcaggt
     3961 aaaggtgaag gtacttcgtt aattgtcaga gagtttggaa tgctattagc gaagcagaac
     4021 aataagtcag tactacttgt cgatacatgt ccagggcatg cggatcagca tcaggcattt
     4081 catgtggaac ccaaaattcc attggaatcg accattaggc acggaggctc gcttgatctg
     4141 tcaatttcaa gaatccccca ccctcaacaa tctctttccc tttgcttcct ttcagaaaat
     4201 catgactcga attcaagcac gtcatggtta tcgcgagata tggtcccatg ggaaccagtc
     4261 caaaagcagt tcgactatat cctcattgac tctccaccaa taagctcctc acatgatggt
     4321 ttggccctct gctcgttggt tgatggtgtt gttctcgtaa tggaagcagt gcattcccgt
     4381 tcccacgtag ttcaaagctc aagagataaa ctcatcgaaa gtggtgggaa cattcttggc
     4441 attgcgttta ataaacaaaa acgatacata cctgactgga tttacaggag actnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntattcta cgcaatggga tttttttgta
     4621 cgttccttat accattacaa atctttgccg ccatcggagc accaaacacc ctccaagcga
     4681 cgcaatcgga tagggttatt catgtcaata acttcccgcc tcacgcatca gactccaatc
     4741 caggaacgaa ggcattgcct cttaagacca taggaatggc aacaaccctc gctgtagaaa
     4801 atcataaaag aggattcagt acaaaaattc ttatatatcc cggggtttat cgagagaaaa
     4861 ttcatatgga attcagcagc aggaaactgg atccgactat tacatttgaa gctattgaac
     4921 aggggaagac cattatttcc ggttcagaaa tctgggaaaa ctgggaggga acaaaactta
     4981 aaaatattta cacacataaa tggcctttca aatggggagt gaggccgtat cctcctggat
     5041 gggaaggcaa aaccattctc caaccaattg tccgacgtca ggaaatggtt tttgtgaatg
     5101 gaatcaagtt caatcaagta gatttacatt ctgagatgaa gcctggagat ttttcggtct
     5161 cagaggaaaa ttcggaagtg aggatttttt taccagacaa tgttgacata aagcagagcc
     5221 aggttgaaat agcagttcag aaggggctct ttaccattaa aggaaaaagc aattttgtct
     5281 taaaggggct aagatttcaa catgacgcat ctggtcttgt cggacacgcc ctcggaatca
     5341 ccaatgcgaa aaacgtttac cttgaagatt gtgtgttcgc ctggaataac tggggaggat
     5401 atcaattcta taatgtacaa aatgtgacaa cacgacgaaa tgtctcgaca cacaatggag
     5461 gtcccgggca cacagcctgg agaataaaaa actatctttc tgaagatgat gagacctcat
     5521 tcaataactg gagaggggca aagggtaatt tcgtttactg ggcagttgcc ggaacaaaaa
     5581 ttatggggat acacaatgcc acctttcgtc gtcatcgggc ccaaggaaat cacactgcag
     5641 gtttttggct agactacgat aacaagaaca ttgaaattga agatggggtc tcctgtggaa
     5701 atctcaagca tggcttattc cttgaggcca cccaaggccc tctaacaata acaaacatga
     5761 agatttacca aaaccatgaa tggggaatca ggattgtgaa ttcaagtcag atcaatgtta
     5821 cagaaagcca ggtttaccac aatcgagggc cccagattcg agtccaagga caaaagacca
     5881 gaggggtaaa cgactgggaa acaggagaga gacttaacct tggtgcagaa aaatggacgt
     5941 tagagaataa catcattgtt ggtgatggac tattagtcca aagcagtttc aagaatccct
     6001 taggaccggg attgaagtct aacgggaatc tttggtctag cgcatccaac accaaaccat
     6061 ttcaagcggc tgccgaccgc ctgacattta gtgaatggca aatttcaatg aaccaagatc
     6121 agacatcaac ctttgccttt cccgtacaac ccgagtcagg ccaagccctt ttagataatt
     6181 gcacctatcg gggtctcgac tgaattccat ttggcccaca agatttcatt cgacgtcttt
     6241 tagaatattg caagtttaat tttctgtcct ttttttcccc gttgaaggtc ccttaatcct
     6301 tttgctatgg agtgccgctc tatctgggtt ctcctcttcc ttgaaattct tctaatacat
     6361 ccacaaaatt gatccgccac atttcaggtg tatagttgta ggattataat tgcatgagtt
     6421 caaaaaagaa tgtccataat aattgaaata gaatcttcca ttaagcacca aagttgttta
     6481 gtatgaaatg atgaaggcct cttaatatta ctttttgcaa atttttcagg tgaaatcaaa
     6541 tattgccatt gaaagtattc ctatcgttac cttgaatttt taactcatcc attttcctca
     6601 tccattctct tcattcttta tcattttttc ccttttttct gagttactta aatccctaag
     6661 tcggcattgg tcaagaacaa tagatcgtca gctgag
//
LOCUS       DAORAQ010000140         6667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1725_length_6667_cov_64.152299, whole genome shotgun sequence.
ACCESSION   DAORAQ010000140 DAORAQ010000000
VERSION     DAORAQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1725_length_6667_cov_64.152299"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="PKM72_10980"
     CDS             complement(<1..1781)
                     /locus_tag="PKM72_10980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP61358.1"
                     /translation="MNNGKDNYIAEMRFRAVAQSSHDAIIIADQSGTILFWNKGAKDI
                     FGYDSEETVGQPLTMLMPDRYRLAHQAGLERYSTRGETRILGETVELSGLRRTGEEFP
                     LELTLSAWEEDRRLFFSGIIRDISQRKEAEEALQQSEEKYRAIFNQAVEGIYQATPAG
                     VFLNANAALSHLLGYDSPQILMEAIKDIGRQLYVEPRKREEFCRLLEQKDVITDFESQ
                     VYRADGTPIWISENARVIRNAEGAVQWYQGFLVDISGRKQAEELLERQNRLQAENRYL
                     QEEVLEAGAFGDLVGQSPALHNVIRQIDLVAPTEATVLILGESGTGKELVAREIHKRS
                     QRKDQPLIRVNCASIPRELFESEFFGHVKGAFTGAVKDRAGRFGAADGGTLFLDEVGE
                     IPLDLQGKFLRVLQEQQYERVGEERTRHVDVRVIAATNKNLKQEVETGGFRQDLYFRL
                     NVFPLEMTPLRERKEDIPLLAEYLLGVTSKKLHCVQPRLTEALVGQLQRYNWPGNVRE
                     LQNVIERGLILSGQQGLSFDIPQSGEGRSSSQHREPKASARVLSVVPEQEMRRRERDN
                     TLAALQEADWKIYGKGGAAERLGIKPTT"
     gene            2105..3076
                     /locus_tag="PKM72_10985"
     CDS             2105..3076
                     /locus_tag="PKM72_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNP61359.1"
                     /translation="MKNRHSGRMGLAIALLAMTGSVSTFVSQVEAKKVTVELTAVETE
                     VVIDGKGTTYKAWTFNGQVPGPVVRVTEGDQVHFILKNHENNSRPHSMDFHAAETDFL
                     ANYRDVNPGESLEYTWEAKRPGVFAYHCGAFPMIQHIARGMFGAVIVDPKDPAAMPKA
                     DREYVLVQSELFTKDPDDVQAMMDAKNDHVVFNGGIFKYDPVHATKGGEFLTAKPGER
                     VRFYFVNIGPNNFSSLHPIAEIWDDVWASGNPANRMQGVQTQVIGAADGAILDVVVEQ
                     KGVYPIVTHSLKDALTGAIALLEVSDEAEKLPLMPSVGPKAAETTSK"
     gene            complement(3322..3681)
                     /locus_tag="PKM72_10990"
     CDS             complement(3322..3681)
                     /locus_tag="PKM72_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61360.1"
                     /translation="MGPTKVIAKAGALYEVKTDKLVQDGLPTRTEQESYAAQHYIALP
                     VIDKAGKQWVLEGQPVFCLHGTQYETVDDQKVHLARCPDCGGMGIRDDEPLVESDCIR
                     CVQCGHEFDTRLEMMET"
     gene            complement(4265..4918)
                     /gene="elbB"
                     /locus_tag="PKM72_10995"
     CDS             complement(4265..4918)
                     /gene="elbB"
                     /locus_tag="PKM72_10995"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNP61361.1"
                     /translation="MKKVAVILSGCGFLDGAEITEAISTLIAIGQNGAEYEVFAPNKD
                     VEETNHLTQKPTGQKRNVLQEAARIARGEIQSLEQLKAENFDALAFPGGFGAALHLCD
                     FGKKGSGGQIDPQVARIVKEFSDSHKPIAAICIAPAIMALALGKKGINVTIGEDAGTA
                     FELEKTGAKHQNCAVEKYVVDHSNKVITTPAYMYGSAKPHQIFAGVSGAIAELIKMA"
     gene            5213..5539
                     /locus_tag="PKM72_11000"
     CDS             5213..5539
                     /locus_tag="PKM72_11000"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HNP61362.1"
                     /translation="MNHQRFDFRGTGFSCLWLFVWTWVVTVITFGLFFPWAYSAQQRW
                     ISEHTFIGNRQLAFQGTGIGFFGTWLLIMVLTIITFGLYMPWGFCRLKRWQTNNLAFA
                     DEEFPG"
     gene            5556..>6667
                     /locus_tag="PKM72_11005"
     CDS             5556..>6667
                     /locus_tag="PKM72_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP61363.1"
                     /translation="MTILILAVGSYGDVLPLVGMARQLRQRGHTVTVFTSAHFTELVQ
                     KAGVEFVPLGTAEDYDAMADNPALWHPHKGWRLIMKRLVSSALEETYDLLKSKVIKGN
                     TLLVSSTLGFAARLIQETHHIPHATVHFSPGVFHSAHQSPKIPGLPLPDWLPVAFKRH
                     MWKFLDHTIIDPVVKSRLNRFRRQLGLPPVSRIFHDWLHSPDLVLGLFPEWFAAPQPD
                     WPPGTHVTGFPLYDEAPDSVLPGIVQNFLDAHPEPLIYTPGSANKHGQAFFKEAAEAS
                     QELGQAAIFLTRYPEQLPPSLPTGVTHFSYVPLSQLLPHAAAIIHHGGIGTCSQALRA
                     GTPQLIQPLAFDQFDNAARVEKLGVGRMIRKRNFKAS"
BASE COUNT         1537 a         1839 c         1522 g         1769 t
ORIGIN      
        1 gtggtgggtt taattccgag tcgttcggct gcgccgcctt tcccataaat cttccaatcc
       61 gcttcctgaa gagccgccag ggtattgtcc cgttcccgac gccgcatttc ttgttcgggc
      121 accacactca agactctggc cgaagccttg ggctcccggt gctgtgagga ggatcgccct
      181 tctcccgatt gaggaatatc gaacgacaat ccctgctgtc cggaaaggat cagacctcgt
      241 tcaatgacgt tctgtaactc tcgaacattt cccggccagt tatatcgttg taattgcccc
      301 accaatgctt ccgtcagtct tggttgcaca cagtgtagct ttttggatgt cactccgaga
      361 agatactcgg ctaagagcgg aatatcttcc tttcgttcgc ggaggggagt catttcaaga
      421 gggaagacat tgagccgaaa atacaggtcc tgccggaatc caccggtctc cacctcctgt
      481 tttaaattct tgttggtcgc ggcaatcact ctgacatcga catgtctggt gcgttcctct
      541 cccacacgtt catattgttg ctcctgcaag accctcagaa atttaccttg aagatccaag
      601 ggaatctccc caacctcgtc gaggaacaag gtgcccccat ctgcagctcc aaatcgcccg
      661 gcccgatctt tgaccgctcc ggtgaaggct cccttcacgt ggccgaaaaa ttcactttca
      721 aacagctccc gaggaattga ggcacaattc actcggatta acggttggtc tttccgttga
      781 ctccgtttgt gaatttccct ggcaaccaat tccttaccgg ttccggattc accgagaatc
      841 aacacggtgg cctccgttgg tgccacaaga tctatttgac ggatcacatt gtgcagagcc
      901 gggctttgtc ccacgagatc cccgaaggcc ccggcttcga gcacttcttc ctgcaagtac
      961 cggttttctg cttgcaaccg gttttgccgc tccaagagtt cttcggcctg ttttcgtcca
     1021 ctaatatcaa ccaggaatcc ttggtaccac tgcacagctc cttccgcatt gcggattacc
     1081 ctggcatttt ctgaaatcca gattggtgtc ccgtccgccc gatagacctg cgactcaaaa
     1141 tccgtgatga catccttttg ctccagcaac cgacaaaatt cctcccgttt tctcggttcc
     1201 acatatagtt ggcgaccgat atctttgatg gcttccatta atatttgggg ggagtcgtat
     1261 cccaataaat ggctcagggc ggcattggca ttgaggaaaa caccggcagg tgtcgcctgg
     1321 tagatccctt ctaccgcttg attgaaaatg gcacgatatt tttcttccga ttgctgaagg
     1381 gcttcttcgg cttcttttcg ctgagagata tctcgaatga ttccgctgaa aaaaagccga
     1441 cggtcttctt cccaggccga aagcgtgagt tcgaggggga attcctcgcc ggttctgcgc
     1501 agaccactca gttccacagt ctctccaaga attcgcgtct cccccctcgt gctgtatcgc
     1561 tcaagcccgg cttgatgcgc cagccggtat cgatctggca taagcatggt aagaggttgg
     1621 ccgacggtct cctcactgtc atagccgaag atgtctttgg cccccttgtt ccagaacagg
     1681 atggtccctg attgatccgc gataatgatg gcatcgtgag acgactgggc aacggcccga
     1741 aaccgcattt ctgctatgta attgtctttt ccattgttca ttgactcgtt ggtccttatc
     1801 cggttgtagg tctctttcat ctataaccgg gctactgaaa tgttgaaaga taacgcagca
     1861 ataatcatat taaacaacaa tattatgttg ttgttcagca aaatattgct atgcaacaaa
     1921 ttattgttga ataaaaattt aaattaatca aactaaacac ttataaatca ataacttatg
     1981 aatttttcat gagtggcaca taatctgcta taaagactat acatatggtg ttgcatctat
     2041 gggcatagat aggcagacat catcttcaat catcaaattt atgttcgtat gaaaggatta
     2101 aagaatgaaa aatcgacatt caggccggat gggattggcc attgcgcttt tagccatgac
     2161 tgggagtgtt tctacctttg tctcacaagt cgaggccaag aaagtcacag tggaattgac
     2221 cgcagtggaa acggaagtgg tcattgacgg caaaggcacc acctataaag catggacctt
     2281 taacggacag gttccaggtc ccgtcgtacg cgtgaccgaa ggtgaccaag tccatttcat
     2341 tctcaaaaac catgagaata atagtcgccc ccactccatg gactttcatg cggctgaaac
     2401 ggatttcctc gccaattatc gtgatgtgaa ccctggcgag tcgctggaat atacttggga
     2461 ggctaaacgg ccaggagtct ttgcctacca ctgtggggcg tttccaatga ttcaacatat
     2521 tgcccgtggc atgttcggcg ccgtcattgt tgacccgaaa gatcccgctg cgatgcccaa
     2581 ggctgaccgg gaatatgtgc tggtgcaatc agagttattt acgaaagatc ctgatgacgt
     2641 ccaggcaatg atggacgcaa aaaatgacca tgtcgtgttc aacggcggga tatttaagta
     2701 tgaccctgtt catgccacaa aaggaggaga attcctcacc gccaagccgg gggagcgggt
     2761 acggttttat tttgtcaaca tcggccccaa taatttttcc tcacttcatc cgattgcgga
     2821 aatatgggac gatgtatggg ccagcggaaa tccagctaac cgcatgcaag gggtccagac
     2881 ccaggtgatc ggtgcggcag atggtgccat tctggatgtc gtcgtggaac aaaaaggcgt
     2941 ctatccgata gtgacccact cactgaaaga tgcgctcacc ggcgccattg ctcttcttga
     3001 agtgagtgat gaagccgaga agcttcctct gatgccttcg gtaggtccca aagccgccga
     3061 aacaaccagc aaatagtaga agaggacacc gtcaggcctc ctctcaaact gaacgctcat
     3121 ctctttgtgg gaggcacctt catcgcctcc cacgaagaat cgggcctgcc cctaccctca
     3181 atacacacat tctcgtcttt tctgcccaac agtttttgcc cggccgggac acaatcgaaa
     3241 acacatcctc aatgacctga acgctgcctc ccaaacagcg ccgctgctca agcctgaaaa
     3301 gacagggatg cctttacact ctcaggtttc catcatttcc agccgggtat caaattcatg
     3361 cccgcattgg acacatcgga tacaatccga ttccaccaac ggttcatcat ctcggatccc
     3421 cattcccccg caatcggggc atcgggctaa atgaactttt tgatcgtcga ccgtttcgta
     3481 ttgcgttccg tgaaggcaaa acaccggttg cccctcaagc acccattgct tcccggcctt
     3541 atcaataacg ggcagagcaa tataatgctg tgccgcatag ctttcttgct ccgtacgtgt
     3601 cggaagacca tcttgaacca gtttgtcggt cttgacttcg tagagtgcac ccgctttggc
     3661 aatgactttt gtcggtccca tcttcacctc ctttagatgt aaacgcaccg ttttgaaatc
     3721 atgttgcaat tggcgaactc ctgggtgtct ggtgaatgct cctgatcctc tttgttgcca
     3781 gacactcacc ctcctttggc agccatcccc tttaattttt catggtttga ggccgaaaac
     3841 atctttatcc gaaaacctcg aaagaaacca ctcatcacca ttaaggctca ttcgtatgcc
     3901 agtctcgcct gtaagacttc tgctgctggt cggagggtgg atcagtcttg gtctggggct
     3961 tttaggaata tttgttccac tcgtgcccac gactcccttt atcctccttg ccgcttggtg
     4021 cttctcaaaa agttctcctc gcctgcatgc acagctactc aaacaaccct tcttgggctc
     4081 aatgattcag aattgggaac aagaaggtag catcagtcgg aatgccaagc tcatggccac
     4141 ggcttgcatc attgccctgt tcgggtgctc tcttcttgcc ttcccctttt cgattctcct
     4201 cgccatcttt ctcgtctgca ttggcattgg agttctaatg tttatctgga ctcgacccaa
     4261 gccctcatgc catcttaatc aactcagcaa tcgcaccact gacaccggca aaaatctggt
     4321 gaggcttggc tgagccatac atataggcgg gcgtggtgat aactttattg ctatgatcta
     4381 cgacatattt ttcaaccgcg caattctgat gtttggcacc ggttttttct aattcgaatg
     4441 cggttcctgc atcttctccg atggtcacat taatgccttt tttccccaga gccaatgcca
     4501 tgatggccgg cgcaatacaa atggccgcga tgggcttatg actgtcgcta aactctttca
     4561 cgattctggc cacctgtgga tcaatttggc ccccactccc cttttttcca aaatcacaga
     4621 gatgcaacgc ggctccgaaa cctccaggaa atgccagggc gtcaaaattc tctgctttga
     4681 gttgttctaa cgactgaatc tcgccacgag caatgcgggc ggcctcttgc aacacatttc
     4741 gcttctgtcc tgtgggcttt tgagtgaggt gattcgtttc ctcaacatct ttattgggcg
     4801 caaagacctc atattcagct ccgttttgac cgatggcaat gagcgtgcta atggcttccg
     4861 taatttcagc tccatctaaa aacccacaac ctgaaagaat caccgccact tttttcataa
     4921 tttcctcctt ttccctgaat gcaccgtaga agagacacca gcttcaaccc gaaccgtcca
     4981 aaaatttcgt gataggtgtt acgctagtat gactctggga tgaaggtcaa tctctgaata
     5041 ttcctgaatt ctgttctgag tccggaaact tcactccctg gtatgtccgc aaaaaggaaa
     5101 acgactcctc ttatctccca gcccgcagca aaagcttggt cattcaccca ccagctctct
     5161 catggtacgc tgtgaaaagt ttttctatta caccttttca atgaggaaac gcatgaatca
     5221 ccagcgtttt gattttcgcg gaacaggttt cagttgttta tggttatttg tctggacgtg
     5281 ggtggtgacg gtgatcacct ttggtttatt ttttccatgg gcctattccg ctcaacagcg
     5341 gtggatatcg gaacatacct tcatcggtaa tcgccagctc gcctttcagg gaacaggaat
     5401 tggatttttt ggaacctggc tcctgatcat ggtgctcacg atcattacct tcggactcta
     5461 tatgccttgg ggcttttgcc ggttaaaacg atggcagacc aataatctcg ccttcgcaga
     5521 tgaggaattc ccagggtaac tcctggtatg catcaatgac tattcttatc ctggcagtgg
     5581 ggagttacgg ggatgtgctg cccttggtgg gtatggccag acagctccgg caacgcgggc
     5641 atacggtcac ggtcttcacc agtgcccatt tcacagagct tgttcaaaaa gcgggagtgg
     5701 agtttgtccc cctgggaacg gccgaggatt atgacgccat ggccgataat cccgccttgt
     5761 ggcatcctca caaaggctgg cgactcatca tgaagcgact ggtctccagc gccctggaag
     5821 aaacctacga tcttctcaag tcaaaagtca tcaaaggaaa caccctgttg gtgagttcca
     5881 ccttgggatt tgccgctcgc ctcattcaag aaacccacca tattccgcat gccacggtcc
     5941 atttttcgcc gggcgtcttt cactcagccc accaatcccc gaaaatcccc ggcctgccac
     6001 ttccggattg gttgcccgtt gcattcaaac gccacatgtg gaaatttctg gatcacacaa
     6061 tcatcgatcc tgtggtgaaa tctcggctga atcgttttcg ccgccagctc ggcttaccgc
     6121 cggtgtcgcg gatcttccac gactggctgc attcacccga tctggtctta gggctcttcc
     6181 ctgagtggtt tgccgctcct cagcctgact ggcctccagg aacacatgta accgggtttc
     6241 ctctctatga tgaagctccg gacagcgtcc tccccggaat cgtccagaat ttcttggatg
     6301 cacacccgga accattgatc tacaccccag gctcggccaa taaacatgga caagcctttt
     6361 tcaaggaggc ggccgaagcc agccaggaac tgggacaggc ggcgattttt ctgacgcggt
     6421 atccggaaca acttccccct tccctcccga cgggagtgac gcacttttcc tatgtgcccc
     6481 ttagtcaact cctcccgcat gcggctgcca tcatccacca tggaggcatc ggcacctgct
     6541 cccaagcctt acgggcagga accccgcaac tgattcaacc cctggcgttt gaccaatttg
     6601 ataatgcggc tcgtgtggaa aaattaggag tgggtaggat gattcgaaaa cgaaacttta
     6661 aagcttc
//
LOCUS       DAORAQ010000141         6595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1746_length_6595_cov_68.573547, whole genome shotgun sequence.
ACCESSION   DAORAQ010000141 DAORAQ010000000
VERSION     DAORAQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_6595_cov_68.573547"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKM72_11010"
     CDS             <1..773
                     /locus_tag="PKM72_11010"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP61364.1"
                     /translation="GYYGGSRHKVTSIGQAVTLLGFSTIANLALSFCFYRLIKDMKEV
                     STSGIDHVKFWRRSILASVAARAIGKHFSFPDVELMFLGALLQDIGLLALNEVASDEI
                     ASLQEHNQDNHATLHQYELEHLGVDHSQVGAWLAQHWQLPEEFQVAILYSHTPELFET
                     PLDFQPLIDTVALSGIIADIWTNPDTNAAIASVQKNSQGRLTVEPEDWEPILGHIMAG
                     IPQIASFFHSRIGNFEDMAHIHQLALQQLSSTDPQLFG"
     gene            complement(800..1951)
                     /locus_tag="PKM72_11015"
     CDS             complement(800..1951)
                     /locus_tag="PKM72_11015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNP61365.1"
                     /translation="MLGSWSIGKKDQSAHGSQKEQDAIESRPTGELGESDKAAETLAR
                     VLRCLGRHAIELDHLSEEEIEKEFERWARHVLVGAQIGDGTKNRDASPRRDWGALTQF
                     VNGHRQQEKSYVTRNLQDMRNVLCEFTNIMGRTFVEDQETDQQVSNHLGQLRTAIEDG
                     SLQDVKQALLAVVENISALVDERKERQQQRLESLGEKLRVVEEELGGARKLMTLDALT
                     QLYNRGALDLQLERTAGMSFFSGTLACILIVDVDHFKHVNDTYGHPAGDVVLQQLAKR
                     LVSTFPRKTDFIARYGGEEFCVLLQGAGEELSQRLGERLLDAVRGEEFSYQDITIPVT
                     ASIGLAELLPGEPVFSWIERADRALYRAKDNGRNQLCLATENVGVGKKD"
     gene            2176..2943
                     /locus_tag="PKM72_11020"
     CDS             2176..2943
                     /locus_tag="PKM72_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNP61366.1"
                     /translation="MNQPHPISSRQNAHFKRWVSLLDSHGIKRHRLCLVSGHKLRKEI
                     GSNSEVTVHEILLPPSWSYREDLPSQAIPYQLSHSLFQELDVFGTKEPLLVCGVPEMS
                     QTNLSRPPNGLEVLCPIGDPGNLGALLRCCRAFDVRTVVLLQEAVHPFHPKVIRASSG
                     AVFVQSLQWADSITDLNAPDTLQWITALDLHGTNISTVKWSKHVRLLIGEEGVGVPPY
                     QFSERLCIPQIHPSIPLNATVAGSIALFAYRQYHSQD"
     gene            3015..3272
                     /locus_tag="PKM72_11025"
     CDS             3015..3272
                     /locus_tag="PKM72_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNP61367.1"
                     /translation="MNTPHSPQDQKLPTIQPGRYRHYKGKEYQVIGVAKHSETEEELV
                     VYRALYGDCGLWVRPAKMFCEKIEMEGKLVCRFERIDDVKN"
     gene            complement(3544..4032)
                     /gene="purE"
                     /locus_tag="PKM72_11030"
     CDS             complement(3544..4032)
                     /gene="purE"
                     /locus_tag="PKM72_11030"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNP61368.1"
                     /translation="MAESSFQVAIIMGSQSDWEIMQLASETLTKLHINHESRVLSAHR
                     APDALLEFISEAESCGVQVFIAGAGGAAQLAGVIAAKTQLPVLGVPMQSKSLQGLDSL
                     LSIVQMPRGIPVGTLAIGSAGAVNAALLAAAILALADDSLSRRLQAYREEQTRSVLAE
                     QL"
     gene            4282..4917
                     /locus_tag="PKM72_11035"
     CDS             4282..4917
                     /locus_tag="PKM72_11035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNP61369.1"
                     /translation="MAIRLGDEAPNFTAETTQGTVNFHEWLGDGWGILFSHPKDFTPV
                     CTTELGTMANMTEEFKKRNVKVLAISVDPLDSHNGWVKDINETQHCTVSYPIIADPEK
                     KVANLYDMIHPNALDNMTVRSVFVIGPDKKVKLTLTYPASTGRNFNEILRVIDSLQLT
                     AKHKVATPANWKDGEDCIITPAVTDAEIPALFPKGHRAVKPYLRFTPQPNR"
     gene            complement(5302..5646)
                     /locus_tag="PKM72_11040"
     CDS             complement(5302..5646)
                     /locus_tag="PKM72_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61370.1"
                     /translation="MSVHKSGAFLQQCFSVHPLCLNVKLVSPPQIVGVLCTNCQMRHR
                     LTLPEAPVSSEETTGIDSHELLRLQECVQSHPKEVRVSLVHIEQSAVEFRCGSCHCNY
                     ALNVALFETHQS"
     gene            complement(5733..6185)
                     /locus_tag="PKM72_11045"
     CDS             complement(5733..6185)
                     /locus_tag="PKM72_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61371.1"
                     /translation="MAESSGGMSPHRFDVNLFIPGLVLMLCLGLVACSHWRDSYFDDG
                     VGELTQSDIQAKLGKPHMVKDPLLSDKTTWTYRFAMSESELDPSGVKALGKQAGSLMG
                     GAQGGPREKVFCFMYILTFDKEGILRHWERDVCPIPQPPDPFQSGLSG"
     gene            complement(6185..6367)
                     /locus_tag="PKM72_11050"
     CDS             complement(6185..6367)
                     /locus_tag="PKM72_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61372.1"
                     /translation="MIARVIGILCVAVGFMLGIQGLEHPGSPMLPTALGMIATGLVAQ
                     VFALVKACFAGSQGKG"
     gene            6366..>6595
                     /locus_tag="PKM72_11055"
     CDS             6366..>6595
                     /locus_tag="PKM72_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61373.1"
                     /translation="MMEPDFPLDPATGGDFSEEDGIWTGSFDILKPSGFGQFSVSDLM
                     GTFSSLPQSLLAISLIFTVIAFIPTHLAVPFKG"
BASE COUNT         1744 a         1768 c         1431 g         1652 t
ORIGIN      
        1 cggggtatta cggtgggtct cgacacaagg tgaccagcat cggacaggcc gtgactctgc
       61 ttggattttc aacgatcgcc aatctggctt tgtctttttg cttttatcgt ttaatcaaag
      121 atatgaaaga ggtctcaact tccgggattg accatgtaaa attttggcgg cgatccattc
      181 tggccagtgt ggcagctcgt gccattggaa aacacttttc ttttccagac gtagaactca
      241 tgtttcttgg tgccctgctt caggatatcg gcctcttagc attaaacgag gtggcctcgg
      301 atgaaattgc ttcgctccag gaacacaatc aggacaatca tgccacatta caccagtatg
      361 aactagaaca cttgggagtc gatcattcac aggtgggagc ctggctggcc cagcattggc
      421 aacttcctga ggagtttcag gtggccattc tctatagcca tacacctgaa ttatttgaga
      481 ctccgttgga cttccagcct ctgatagata ccgtggcact ctccggtatc attgcggaca
      541 tctggaccaa tcccgatacc aacgctgcca ttgcctcagt acagaagaac agtcaggggc
      601 gcctaaccgt tgaaccggaa gactgggaac cgattctggg gcacatcatg gcaggaattc
      661 cacaaattgc cagttttttc cacagccgga ttgggaactt tgaagacatg gcccacatcc
      721 atcaattagc cttacaacaa ctgagctcta ctgatcctca attattcggc taaacgaagc
      781 tcatacctac cattctgact cagtctttct tccccactcc aacattctca gttgccagac
      841 acaattgatt ccggccattg tcctttgccc ggtacaacgc acgatctgca cgttcgatcc
      901 atgagaacac gggttcccca gggagtaact ctgccaatcc gatcgatgcc gtgactggaa
      961 tagtgatgtc ttggtaggaa aactcctctc ctctcacagc atccaacaga cgctcaccca
     1021 atcgctggct cagttcctct ccggctccct gtagcaaaac acaaaactcc tcccctccat
     1081 atctggcaat aaaatccgtt tttctgggaa aggtcgacac caggcgcttc gccagttgct
     1141 gtaacaccac atcgcctgcc ggatggccat aggtatcgtt gacatgctta aaatgatcga
     1201 catcaactat taaaatacag gccaacgtcc cggagaagaa actcatgccg gccgtgcgct
     1261 ctaattgaag atccaacgct ccgcggttat agagttgagt gagagcatcc agcgtcatga
     1321 gctttcgtgc gccacccagc tcctcttcaa caacacgcaa tttttctcca agactttcca
     1381 agcgttgttg ctgacgttct tttcgttcat ccacaagagc actgatattt tcaaccacag
     1441 ccaagagggc ttgtttcaca tcctgtaaag aaccgtcctc gattgccgta cgtaattggc
     1501 ccaaatgatt ggatacttgt tgatcagttt cctgatcctc cacaaatgtg cgtcccatga
     1561 tattggtaaa ttcgcaaaga acatttcgca tatcttgaag attgcgtgtc acataggatt
     1621 tttcctgttg acgatgtcca ttgacaaatt gagtgagtgc cccccaatcc cgtcgtgggc
     1681 ttgcatcgcg attttttgtc ccgtcgccga tctgggcacc caccaataca tgtctcgccc
     1741 accgttcaaa ctccttttct atctcctctt ccgaaaggtg gtctagttca atcgcatgac
     1801 ggcccagaca tcgcaaaacc cttgctaagg tttctgcagc cttgtcggat tctcccaatt
     1861 ctcctgtagg cctagattca attgcatctt gctccttttg ggacccatga gcagattgat
     1921 cttttttccc aatggaccaa gatcctaaca tgtcgtcctc ctcttcaagt cacttctgcc
     1981 tatatgtgtt tcagtaaaac atccagcgga tgcatgggcc gagcggggta actgtttcat
     2041 tcggttatcg gcatttactc taggtctctt gagtcacttt ttggtatgct ctctcctaat
     2101 aactacaaac agtaggccta aagggatttc ttggcccccc gtttacctcg tttacttcaa
     2161 tttctctaaa ggctcgtgaa tcaaccacac ccgatctcca gtagacaaaa tgcccacttc
     2221 aagcgatggg tatcattact tgactctcat gggattaaac ggcatcgcct ctgccttgtt
     2281 tctgggcata aactccgaaa ggaaatcgga tcgaattctg aggtgacggt ccatgaaatc
     2341 ttacttcccc catcgtggag ctacagagag gacctcccat ctcaggccat cccttatcaa
     2401 ctgtctcatt ccctgtttca ggaattggac gtctttggaa cgaaagaacc gctcctggtc
     2461 tgcggcgttc ccgaaatgtc tcagacaaat ctaagccgac ctccaaacgg attggaagtc
     2521 ttgtgtccaa ttggagaccc tgggaacctg ggggccttac tccggtgctg tcgagcgttt
     2581 gacgtgcgaa cggtggtgtt gcttcaggaa gccgttcacc ccttccaccc gaaggtcatt
     2641 cgtgccagta gcggcgcggt atttgtccaa tccttacaat gggcagactc cattaccgac
     2701 ctgaatgctc ctgacacatt gcaatggatt accgctttag atttgcacgg gacaaatatc
     2761 tctaccgtga aatggtctaa gcatgtccga ctccttattg gagaggaagg cgtcggtgtg
     2821 ccaccctatc agttttcgga acgcctatgt attcctcaaa tccatccctc tatcccatta
     2881 aatgcgacag tcgcaggaag tatcgccctc tttgcctatc gccaatacca ctcacaggac
     2941 taatcaatgt cgatcggacc gtacttcgta accggttgtg gcaccattcg aacagaacaa
     3001 cacccatcca tcttatgaac acaccacact cgcctcaaga ccagaaactc ccaacgattc
     3061 aacccggtag atatcgtcac tataaaggga aagaatacca ggtcattggc gtcgccaaac
     3121 attccgaaac agaagaagag ctcgtggtct accgcgcact ctatggcgat tgcggccttt
     3181 gggtcagacc ggccaagatg ttctgtgaga aaattgaaat ggagggaaaa ctagtctgca
     3241 gatttgaaag gatcgacgat gtaaaaaact aaaatggacg ccacgaccat caaaccggga
     3301 ggagatgact cgcaactcgt ctgatcagac agaatgtctt tcctgttcgg atacaagaaa
     3361 ggcgatttac atcgtggtac ccatccctcc caattctggg ataggttgcc ttgtaaatat
     3421 cgggcagccg aacatgatat ccaacacgct ggctcgggag gcttctatgc actctcctca
     3481 ccaagcgccc tgcctgtcat acaccttttt tcgcaaacta tttgcgaaat tctccaccga
     3541 cactcacaac tgttcggcta ataccgaacg cgtttgttct tctcgatacg cttgtaaccg
     3601 tctggagagg gaatcatctg ccaatgctaa aatcgcagca gccaacaacg ccgcattcac
     3661 ggcacccgcg ctccctatcg ccaaggttcc tacaggaata cctctgggca tttgaacaat
     3721 cgacaagagg gagtcgaggc cttgcaagga ctttgattgc atgggaacac ccaacaccgg
     3781 caattgggtt ttcgccgcaa tcactccggc taattgggct gcccccccag ccccggcaat
     3841 aaacacctgc actccacaag attccgcctc actgataaac tccaacaaag catccggagc
     3901 cctatgcgca gagaggaccc gactctcatg gttaatatgc agcttagtga gcgtctcact
     3961 ggccaactgc ataatttccc aatccgattg actccccatg ataatcgcga cctgaaatga
     4021 tgactcagcc ataacctctt gtactccttc ttaatttgtt attcttatag gacaatgggt
     4081 ggggaaaaag tccatgtctc aattaaacgg gcttacctta gagcatttcg acccaagttg
     4141 accatataac gggaactatc ttacactaaa atgcctctga attttttgag gaaattccca
     4201 ataggagcca accaattttc tcgcgggttt tctaccgcag agatataata ctcactttat
     4261 tcataatggg agagaaataa tatggcaata cgtcttggag atgaagcacc aaattttaca
     4321 gcagaaacga cgcaaggcac cgtgaatttt catgaatggc ttggggacgg atggggcata
     4381 ctcttttcgc atcccaagga tttcaccccg gtttgcacca cggaattggg aacaatggcg
     4441 aacatgacag aggagtttaa aaaacgaaac gtgaaagtgt tggctatcag cgtggatccg
     4501 ctcgactctc ataatggctg ggtcaaggac attaatgaaa ctcaacactg cacggtgagt
     4561 tatcccatta ttgccgatcc agaaaaaaag gtcgcgaatc tctatgacat gattcatccc
     4621 aatgccctcg acaatatgac cgttcgctcg gtgttcgtga tcggaccaga taaaaaagtc
     4681 aaattaaccc tgacctatcc ggcctcaact ggcagaaatt tcaatgaaat tcttcgtgtg
     4741 attgactcac tgcaactaac cgccaagcat aaagttgcca ctccggcaaa ttggaaagac
     4801 ggagaagact gtatcatcac gccggctgtg acggatgcag aaattcccgc gctatttccg
     4861 aaaggtcatc gtgcggtgaa accctatctt cgcttcaccc cgcaacccaa ccgctagagc
     4921 ccccaaaaaa actgaggcag cgccacacaa gtgtggcgct gcccgtgatt catccattcc
     4981 ttgtcttccc ctcccctgac aatttctgat ctggcaagtc cgtcttatgg ttcataatgc
     5041 agcatttcta ttaagcagaa ctgtgtaatg attcggtcta cggtcagaat ggcaaacgtc
     5101 ctgagacgat aaaaagccag aaggaaatag gtcctcggaa ttgagcgcga ggggggaggg
     5161 cacggacaga tatgttgaat atccccaatc cggaattgtt tccgttcggt ccttctcaga
     5221 acgctcctca ttggaaaaca gctgtggggc aaccaacaag aagttgataa gggacgggaa
     5281 caaattctag aaaaagatca gtcaggattg atgggtttca aatagggcca cattcaacgc
     5341 atagttgcaa tgacaggacc cacatctgaa ttctacggca gactgttcga tatgaaccaa
     5401 gctgaccctg acctctttgg gatggctctg aacacattct tgaagccgaa gtagttcatg
     5461 gctatcaatt ccggtagtct cttctgagga taccggagct tcgggaaggg tcaaacgatg
     5521 gcgcatctga caattcgtac aaagcacccc gacaatttgc gggggagaaa caagtttaac
     5581 attcaggcac agcgggtgta cggagaaaca ttgttgaaga aaagctccgc ttttatgcac
     5641 gctcatatga ttaatcccat caattctctt gtaaaaagaa tgacctcacc tctggaggat
     5701 ttattactca cttactatat ataggaccaa acttacccgg aaagaccact ttgaaatgga
     5761 tcgggaggtt ggggaattgg acagacatcc cgctcccaat gacgcaggat cccctcttta
     5821 tcaaaggtca aaatatacat aaaacaaaac accttttcac gtgggccacc ttgagcccca
     5881 cccatcaggc ttccagcctg cttgcctaag gcctttactc cagacggatc caattcactt
     5941 tcagacatgg caaatctgta ggtccaagtg gtcttgtctg aaagcaatgg atctttgacc
     6001 atatgaggtt tccccaactt cgcttggata tccgactgtg ttaactcccc cacgccatcg
     6061 tcgaaatagc tatcgcgcca gtgagaacag gcaacgagtc caagacagag catgaggacc
     6121 agacctggaa taaataggtt cacatcaaaa cgatgaggcg acatgccccc agatgattca
     6181 gccatcaccc cttgccctgt gaacctgcaa aacaagcctt cactaaagcg aacacttggg
     6241 cgactaagcc tgtcgcaatc attcctaagg cggttggaag catgggagag ccaggatgtt
     6301 ctaatccctg aatacccaac ataaacccta ccgcgacaca cagaatccct ataactctgg
     6361 caatcatgat ggaaccagat ttcccgctcg atcctgcaac gggaggagac ttctctgagg
     6421 aagatggcat atggacaggt tcttttgaca tcctcaaacc atcaggattt ggtcagtttt
     6481 ctgtatctga tttgatgggg acgttcagca gccttcccca gtcgcttctt gcgattagcc
     6541 tcatattcac tgtaattgcc ttcataccaa ctcacctggc tgttcccttc aaagg
//
LOCUS       DAORAQ010000142         6500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1777_length_6500_cov_56.002017, whole genome shotgun sequence.
ACCESSION   DAORAQ010000142 DAORAQ010000000
VERSION     DAORAQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1777_length_6500_cov_56.002017"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKM72_11060"
     CDS             complement(<1..266)
                     /locus_tag="PKM72_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61374.1"
                     /translation="MKMYIGVCFIMMAAGGCASDKPVPLTALAPPPNVDAQVLQYMTE
                     GKRFFEEGQWESARQQFQLAVQQQADLAEAHYNLGLSMDKMGDQA"
     gene            complement(278..3742)
                     /locus_tag="PKM72_11065"
     CDS             complement(278..3742)
                     /locus_tag="PKM72_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNP61375.1"
                     /translation="MTNPSPLSDADARQIAETTFDRNVVVLAGAGTGKTTLLVNRLIH
                     ALLREPHPLRLTDMLALTFTNKAANEMKARLRDRLHGLLADCEAEAGSAELGGTGLEE
                     FKQRYHVSTAHVRGKIQVALSDLEKSQIATLHSFAAHILRLYPLEARVDPHFQEDDGT
                     QFLQIFQDVWDAWLEQELGAKGSAHAQWRDLLNHMGLKEIRSFAFSLCQDQISIPELE
                     RQVDAEGSPAAFRMWLETKQHQVRSLLTQYDRAKPRKIEKLFSLAERVFDWVGQGESP
                     TDFPLSDAEKVLLGSPIGKAPGEWNASDFQDARRAIQGAQQMLQVNEALLSRVIHLLG
                     PFAARVRQGFSDKGWIRFDGLLIRVRDLLRDHPMVREQLKGTYAAIMVDEFQDTDPVQ
                     YEILLYLGERPHACGQFWRDIQLMPGKLFIVGDPKQSIYAFRRADIEAFDQVVEKVTD
                     DGGIVCTLLTNFRSDGSILEAVNAVFDRLFIPQANVQPPNVPLAVGRMREAGSGSTGV
                     EICVMANPQEEDEWDADRATRVEAEWLAGWIEEQLVPGRQWIMEKGVRTSLRPGHIAV
                     LLRKFTNAQVYLEALQRHNIPCITDGERHFYKRQEVIDVVNVLRVLDDPTDALALVGI
                     LRSSLGGLTDQEIMDVMKLGPLDVRQAHQMNEWGNSKQSVIQGLFQRLAWLHIQANRL
                     PIPELLDHLFLQLPLVELAAASSHGEQAVVNVWKLRDLMSEQAAMPYLSFSAWVERLV
                     EALMTHPPEPEAPLAEETLDAVRVLTIHKAKGLEFPVVILPGLHQRATGPDRGAHISY
                     DWISGLYGCTLPPVWNTGQIPLWEKQRIREAAEQRRVLYVGMTRARERLILSGGILAK
                     RGGESLLGLIQEIAEGEFGDTELRVARVGEVSIPHTVVTPAVLQYLKPSQAHSDAESS
                     SEVVLSTAQWDAREMRWQQANHAEAYVNPSMLHQARSLQDQGERGEISRGTSQRLGTL
                     MHQLLQRWDFQIEPGMVRTALVAFCERQLSGELGKDKDEVIRELETLFDQLVASPVYL
                     ELQQSNILGREVPFAVPWPVGQQEPSLPRICVMEGVMDVIYEINGDVWVGDYKTDHVS
                     SRTMAHRAETYREQAYAYARAASQCLGPVVKGCKLFFIRLGEVVTVDLGVDENMYQNK
                     QQVIRRER"
     assembly_gap    3933..4032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4033..4215)
                     /locus_tag="PKM72_11070"
     CDS             complement(<4033..4215)
                     /locus_tag="PKM72_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61376.1"
                     /translation="MVRSPLHPVMNHSRVRLVDYKVSMRRSFRADEWDLVTNTLQGRQ
                     LQPPLYSFLTSLNLERG"
     gene            5100..5612
                     /locus_tag="PKM72_11075"
     CDS             5100..5612
                     /locus_tag="PKM72_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61377.1"
                     /translation="MSKSESEQNLLTMKAAEARDTAARLAKVTKTHAELLEAQKNDGS
                     LNDPSQWPSVWAGAISSGFGKYAFTTDWTGGAPHPWWTAGAHLTGGDWTAWTTGTWAG
                     AGQFYEDPSNYFGKQVWANLKCGGFGAGGVSLALYSSQGGPIIGQFGGIYTGIGGCNI
                     SCPLTYTAGP"
     gene            complement(6259..>6500)
                     /locus_tag="PKM72_11080"
     CDS             complement(6259..>6500)
                     /locus_tag="PKM72_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61378.1"
                     /translation="SFPGSIWDTPTGDQLLRTIRRWIESIRAGQFFILPGSYCRDCSW
                     SVACRFQHHPSWVRAYGIPLAKEFRQGRKQRAVHG"
BASE COUNT         1549 a         1846 c         1519 g         1486 t
ORIGIN      
        1 gcttgatcgc ccattttgtc catggacaaa cctagattat agtgcgcttc ggccaaatcg
       61 gcctgttgct gaacggccaa ctgaaattgt tgtcgtgccg attcccactg gccttcctca
      121 aaaaaacgct tgccttcggt catgtactgc aacacttggg catctacatt cgggggaggg
      181 gccaaggctg tcaacggcac tggtttgtca gacgcgcacc ctcctgccgc catcatgatg
      241 aaacagaccc caatgtacat cttcatcctc caattcctca tcgctccctc cttatcacct
      301 gttgtttatt ttggtacata ttttcatcga ctcccagatc tacggtgact acttcaccga
      361 gacgaataaa aaagagttta caccctttga ccacaggacc caagcattgg cttgccgccc
      421 gcgcataggc ataggcttgt tcccggtagg tctccgctcg gtgcgccata gtgcgggagg
      481 atacgtgatc cgttttataa tcgccaaccc atacgtctcc attgatttca tagattacat
      541 ccatcacgcc ctccatcacg cagatccgtg gcagggaagg ctcctgttgc ccaacaggcc
      601 atggcaccgc aaagggaacc tcccgaccca ggatgttcga ctgctgcaac tccaaatata
      661 ccggtgaagc gaccaattga tcaaaaagcg tttccagttc tctgatcacc tcatccttgt
      721 ctttccccag ctcgcccgag agctgccgct cacaaaaagc gacaagagca gtccgaacca
      781 tccccggctc aatttgaaaa tcccatcgtt gaagaagctg atgcattaac gttccgagac
      841 gctgactggt cccacgggaa atttcccctc tctctccctg gtcctgaaga gatctcgcct
      901 gatgcagcat agagggattg acgtaggcct ccgcatgatt ggcctgctgc cacctcattt
      961 cacgggcatc ccactgtgct gtcgagagta ccacctcact gctactttca gcatcagaat
     1021 gtgcctgtga aggcttcaaa tattgcaata cagcgggtgt cacgacggtg tgtggaatgg
     1081 aaacctctcc cacacgcgcc accctcagtt ctgtatcgcc aaactctcct tcagcaatct
     1141 cctgaataag acccaacagg ctctccccgc cccgcttcgc aagtatgcca cctgataaga
     1201 ttaatcgctc tcgggctctg gtcattccca catacagcac gcgccgctgt tcagcggctt
     1261 ctcgaatccg ttgcttttcc cacaagggaa tctggcccgt attccacaca ggtggcaacg
     1321 tacagccata caagccgcta atccagtcat aggaaatatg tgccccacga tcaggtcccg
     1381 tcgccctttg atgtaatccg ggcaatatca ccacagggaa ttcgagtcct ttggccttat
     1441 gaatcgttaa gacacgtact gcgtctaagg tctcttcggc caagggggcc tcaggttccg
     1501 gaggatgggt catgagggcc tcaaccagtc gttccaccca ggcagaaaag gataaataag
     1561 gcatggccgc ctgttcactc atgaggtcac gaagtttcca gacatttacc accgcctgct
     1621 ccccatgact cgaagccgca gctaattcca cgagagggag ttgcagaaac aggtgatcaa
     1681 gcaattccgg aataggaagt cgatttgcct ggatatgaag ccaggccagc cgttggaaaa
     1741 ggccctgaat gactgactgc tttgaattcc cccattcatt catttgatga gcctgccgga
     1801 catccaaggg tccaagtttc atcacatcca taatctcttg atccgtcagc cctcctaacg
     1861 aagaacgaag gatacccaca agggccaggg catcggtggg gtcatccagg acgcgaagca
     1921 cattcaccac atcaatcact tcctgacgct tgtaaaaatg ccgctcacca tcggttatgc
     1981 aaggaatgtt gtggcgctgc agcgcttcaa gatacacttg cgcattggta aatttcctta
     2041 acaacaccgc gatatgccct gggcgcaatg aggttctcac tcccttttcc atgatccact
     2101 gtcttcccgg caccaattgc tcttcaatcc atccggccag ccattcggct tccaccctgg
     2161 tggcccgatc tgcatcccat tcgtcctcct cttgagggtt agccatcaca cagatctcca
     2221 ctcctgtcga gccggaaccg gcctctcgca tccgtccaac cgccagaggg acattggggg
     2281 gttgcacatt ggcttgggga atgaacaacc ggtcaaacac cgcattgacg gcttcaagaa
     2341 tggacccgtc actacgaaaa ttggttagaa gcgtacagac gatccctcca tcatccgtca
     2401 ctttctccac gacctgatcg aatgcctcga tatcagctcg ccgaaaggca tagatcgatt
     2461 gtttgggatc gcccacaata aacagctttc ccggcataag ctggatatcc cgccaaaact
     2521 gcccacacgc atggggacgc tcacctaaat agagcaaaat ttcatattga accggatcag
     2581 tatcctgaaa ctcatcgacc ataatggctg cataggttcc cttcaactgt tcccggacca
     2641 tgggatgatc gcgcaggaga tccctgaccc ggatcaagag cccatcaaaa cgaatccagc
     2701 ccttatctga aaaaccctgc cggacccgtg ccgcaaacgg cccaagcaga tgaatgactc
     2761 tactcaatag ggcttcattg acttgcagca tctgttgcgc gccttgaatc gcccgcctgg
     2821 catcttgaaa atccgacgca ttccactctc ccggtgcctt gccaattgga gaaccaagca
     2881 aaactttctc cgcatcagac aaggggaagt ccgttggtga ctccccctgc cccacccaat
     2941 caaacacgcg ttcagcgaga gagaagagct tctcaatttt tcgaggtttg gctcgatcat
     3001 attgcgtgag caatgatcgg acctgatgct gtttagtctc tagccacatc cgaaaggccg
     3061 caggggatcc ttcagcatcc acttgccgtt ccaactcagg aatagagatc tgatcctgac
     3121 agagcgaaaa ggcaaaggat cgaatctcct taagccccat gtgattgagc aagtcccgcc
     3181 actgggcatg cgcggaacct ttggcaccta attcctgttc caaccaggca tcccacacat
     3241 cttgaaatat ctgaagaaat tgagtcccgt catcctcttg gaaatgagga tcgacccggg
     3301 cctctaaggg atacaggcga aggatatgag cggcaaaact atgaagtgtg gcgatttgcg
     3361 acttttccag atcactcaag gcgacttgaa tttttcccct cacatgtgcc gtggatacat
     3421 gatagcgttg tttgaattcc tccaggcctg ttccacctaa ctccgcactt ccggcttccg
     3481 cctcacaatc agccagcaac ccatgcaacc gatcgcggag ccgcgctttc atttcattgg
     3541 cagctttatt cgtaaaggtc agagccaaca tgtcggtcaa gcgtaatggg tggggctccc
     3601 tcaaaagcgc atgaatcagg cgattgacca acaatgttgt tttacctgtc ccggctccgg
     3661 ccagcacgac cacattgcga tcaaacgtcg tttccgctat ctgccgggca tcagcatcag
     3721 ataagggaga gggattagtc attggaggcc ttctgtttgc ggatctgtcg aaaggttctt
     3781 gctagcggaa gcccagaggc gcgtgactaa gacggatgag gctgggaacg accggccatg
     3841 gcatattgac agctgcgaca ataggtacca ggtaagatga aaaattcccg gcacgaatcc
     3901 tttgcacccc accctgaagg gtgcgcctca atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nngccacgct caagattcag tgaggtcaag aacgaatata agggaggctg
     4081 caactggcga ccttggagcg tgttggtcac caaatcccat tcatctgctc gaaaagaccg
     4141 acgcattgaa actttataat cgacgagacg aacacgagaa tgattcatca ctgggtggag
     4201 gggggatcgt accatgcagg ataccctctc gcaataaagg gtttggcacg aaacacccag
     4261 tgccctgagg tgacggtgtc ttcgcgatcg accgcacccc ctgtccgcag tgcggcggcc
     4321 ccttgaccat cttcgcggcc attgacgacc ccgccatgat cgtcaacatt ttcggcacct
     4381 cggtttgccc acgcgtgcgc ctacggcatc agccggtctc aacgaattct tcctgacagc
     4441 ctcatcccat cctggattcc ggttcaacgc cgaagccgac gttctgcctt tgtccagttc
     4501 cccaaccccc acgtcccaag tgcgcaacag actaggcctg attcataaaa aatgggccct
     4561 ccggttctct cgaatggtca cctcacggct ttcagacagc acagcctcgc ggatcgattc
     4621 ctcaacgagg tttttggtac attccggttc agtcaaaaag acctttcaaa gctcctattc
     4681 ctcggcccta tccaatgagg ttatttcggg actttatcat tcagcgtcag cttcacctgt
     4741 tacctttgcg cctcgcttcc tttcgtgcct acacatcaag cacctcgtta cccatatgcc
     4801 tgcaaggctc gataccaggc tcgtggctag cgattacctg gacgggattc acacccgcta
     4861 tgtctttgca gcattgccag gccgcaacgg ttaattaacc ccaagaaata aaaaactagg
     4921 tgcgcaaaac ctgagcctca atcccttttt ttattaacct aactggtaca ttattggcgc
     4981 tctctttggc acattttttc tgtccttgac attattcgcg gtttatcgta attccgatca
     5041 ctctagcaaa aaagggaatt tagtttccct atccacttat caggcatgga ggccatgtta
     5101 tgtcaaagag cgaaagcgaa cagaatctgc tcacaatgaa agccgcagag gcacgggaca
     5161 ctgccgcaag attagcaaag gttacgaaga ctcacgccga attgttagaa gcccaaaaaa
     5221 atgacggctc gctcaatgat ccgtcacaat ggcctagtgt atgggcagga gcgatttcat
     5281 cggggttcgg gaagtatgcc tttaccactg actggactgg aggtgcccca catccttggt
     5341 ggacggcagg agcccatctt acaggcgggg actggaccgc atggacgaca gggacatggg
     5401 cgggcgcggg gcagttctat gaagatccca gtaattattt cgggaagcaa gtatgggcca
     5461 atttaaaatg cggagggttc ggcgcgggcg gagtcagttt ggccctgtat agtagccaag
     5521 gcggaccaat aatcggccaa tttgggggga tttacacggg gattggaggt tgcaatattt
     5581 cgtgtccttt gacgtacacc gcggggccat agggctttgg tgagaaacag attcaattgg
     5641 cggacgcggt tcacatcgac cccattccgc atccaccaaa tctcggtaag caaacagaac
     5701 gggattgcct ggttggagtg gaaataatgg gaaggctgat tcatgaaaaa ttggcccctt
     5761 cggcgtctgc cgaaggggca actcacggct ttcagcccgc acagtccggg ggatggattc
     5821 ccaaccaagc cttggctact tttcggtcca gtgaaaaagg ccttttaaca ttcctattcc
     5881 tactcgcccg atggctttgg attctttggc tgaaggacag gacgaatatg agcctgtcgc
     5941 ggctctcgtt tttttgtcga gtttttctgt gaaccggata gtcatttagg gcttatcttc
     6001 cagtggatca ccatggggca gttactctgt gtcccgatgt ttgcagtcgg cttgttcatg
     6061 attatccggg gctataaacg ggcaccccct ccttcccata tccgctcccc tttcaggctc
     6121 tcttttcaga ttacgagaac acctcaataa ccaggttaaa atccaccacc tccgccgccg
     6181 gaaattgcgc ctccaccaaa tcctggcatt accggagccg aggtgacttg cgcagtggca
     6241 tcggaaagaa gttcgagttt acccatggac ggcacgttgc tttcttccct gtcgaaattc
     6301 tttcgccagt ggaataccat aagccctcac ccacgaagga tgatgctgga accgacaggc
     6361 cacagaccat gaacagtcac gacagtagga accaggtaaa ataaagaact gtccggcgcg
     6421 tatgctttcg atccaccgcc ggattgtcct caacaattgg tcaccagtcg gcgtatccca
     6481 gattgaaccg ggaaacgagg
//
LOCUS       DAORAQ010000143         6466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1785_length_6466_cov_70.522383, whole genome shotgun sequence.
ACCESSION   DAORAQ010000143 DAORAQ010000000
VERSION     DAORAQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1785_length_6466_cov_70.522383"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKM72_11085"
     CDS             <1..468
                     /locus_tag="PKM72_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61379.1"
                     /translation="TMPLIRYNTADLAIAASGECSCGRQLPLMGSIEGRERASILLGD
                     GRLLTTRKILDHLAGTLRLGEFRLYQESLKKFRLELTCHALVGHPGIENEDTDSHVHL
                     TILGHLRKLLGEVDLSIRIVKPWPADGTGKTHAIFRGGNLNEGSLRDTTGAAS"
     gene            590..1735
                     /locus_tag="PKM72_11090"
     CDS             590..1735
                     /locus_tag="PKM72_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61380.1"
                     /translation="MMVEWVISRLSGKAQTLVPRYGFYAWACLGLRGVPVVLLRGPTL
                     QSGHIGTLLVAGHDPWVAYLPNRFFIGEPQRELVGNVSLRDLPALLDRLRDAADLTIV
                     RADRLSAEKFFGKDYLAVPEWIDMRLALPGDLDELVRSNRSIREDMRLVRKHKLQPLV
                     TEGEERFDEFYDSMYVPFSRDRHGAMAIIKGRQELRRFIRKGGILWVMGDNQPLAGVL
                     YERKQDTFNLLAIGMASGEIPLKKRGIMAALYYYSIQHARQLGFAEVNFRAARSSLHD
                     GLLRYKRKWGNALFDNPNSYYDLLIRWNSVNGIVKDFFSHTGLIFRDGERLSAIHADE
                     SQSRRSLWIEGLHRLYLLTESGRQPMIDEEVLVKRSRSHTRFSSTLS"
     gene            1980..3824
                     /gene="asnB"
                     /locus_tag="PKM72_11095"
     CDS             1980..3824
                     /gene="asnB"
                     /locus_tag="PKM72_11095"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNP61381.1"
                     /translation="MCGLCGVVYSDPKRPVDRDMLHHMTNMMSHRGPDGAGYFVDPGI
                     GLGFRRLSIIDLETGDQPISNEDNSVTVVCNGEIYNYKELRQDLEAADHRFRTHSDAE
                     VLVHLYEDYGVRCVDFLRGMFGFAIWDSRHRRLMLARDRFGIKPLSYAIKPGALFFGS
                     ELKSILASGCIDRQVDAGAMKELFAVGFVQAPKTLLCPIRRLPPAHYLLHENEKITIQ
                     RFWDHSFPSRGEEGFQRSAQEWAETVRDKLEESVRLHLRSDVPLGCYLSSGIDSSAMA
                     GLMSREISDPIHTFSAAFEDPLFNEIGPKRIFTDFSGYNLRHHITTCRTADFDRLPEM
                     IWHREDPNISSEGIPHMLLARMAAQQVKVVLTGEGSDEPFLNLPLSVRQLIANIPFLR
                     KKYSRSSRAFAAHATMDLSRYTQIIDNASHRSYDDLFSYHLSNIHSSFNDSEPHFFLP
                     KDFEGRNRFAQLQYLDINVRLSDFITRTLDAASMAYGLEARVPFLDHEFVELCSQIPS
                     DLKMRGLQEKHILRRALEGVLPAEILKRRKRGLSAPFWPWQGLLPEFVADALSESRLH
                     TKGYFNPHSVRHMLQQHQNGKANFGRELIGVLNIQLWDDLFVQGCRPS"
     gene            3821..5185
                     /locus_tag="PKM72_11100"
     CDS             3821..5185
                     /locus_tag="PKM72_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61382.1"
                     /translation="MRCTSVLASNPVSTLYSMLRHPHVGREQLVAFQNERVCRLVAHA
                     YRHVPYYRGLFDRHGLKPQDIRTVEDLGAVPITSRSDLQGLPVDEIIARNVDPRSLIT
                     RASSGSSGRPFIVRRTWLEERLHGAFRWRALHALGLRASDKICYVMGNWNIQRQDQLA
                     KRILDTLGMARRKVIDALQPPEDIVQALQKFKPTVVGGYPGVLARIAQTVNCDVLRSL
                     RLHFVNPGGEVLTPLMRQQIQDGFSAPVYETYGSIEFYLLAWECPTTGEYHSCDDGLI
                     IEVLRDGTPADEGEMGELVGTDLHSFAMPLIRYQLGDVVTKGSQTCRCGQPFSTIRSI
                     QGRMIDYFVLPGDRVVHPYELGVRKVPWIREFQVTQERMEFIVMKVVPFHTPSAEEMA
                     ALVQPVIALLGSDVQFQVVLVDTIPREASGKFRVYRSHIRSAYDAFAWTDQKIVPPGV
                     HDDC"
     gene            5772..6230
                     /locus_tag="PKM72_11105"
     CDS             5772..6230
                     /locus_tag="PKM72_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61383.1"
                     /translation="MANQSFPRFNDWAWWLYEHAPAGLDDVAKRCALWVLPWVALRVP
                     VAILRGPTRHSKRLGIIVVAGLHPWADYLQRRFFACAPRREVVGAVPVWALPSFLKRL
                     AVDADLIVARVDRVSAASFLKTVTLWFPKASAADFCCRSILKSLHRPASA"
BASE COUNT         1447 a         1604 c         1788 g         1627 t
ORIGIN      
        1 acgatgccac tcatacggta caatacggct gatctggcca tagctgcatc aggtgaatgt
       61 tcctgcggtc gacaactgcc tttgatgggt tcaatcgaag ggcgcgaacg agcctcaatt
      121 ttactcggag acgggcgtct ccttacgacc agaaagatac ttgatcacct cgcgggaaca
      181 cttcgcctcg gagaattccg cctttaccag gaaagcctaa agaagtttcg gctggagctc
      241 acctgccacg ccctcgtcgg ccatccaggg atcgagaacg aagacaccga ttcgcatgtc
      301 catctaacca ttctgggtca tctccgaaag cttcttggtg aggtcgatct atcaatcagg
      361 atcgttaagc cgtggccggc agatggcacg ggcaagaccc atgcaatctt cagagggggc
      421 aatctcaacg agggctctct cagagacacc acgggagcgg catcttgaga cgcgacattc
      481 ccatggtttc gtggaaacca ggacagaggg tagttgaacc ttgcgaatgt ggttgcggcc
      541 agtcggaaca aaaacagtgg gcacgaatag gatgaaccat ttaaaaaaca tgatggtaga
      601 gtgggtcatc tcccgccttt ccggcaaggc acaaaccttg gttccgcgat atggttttta
      661 cgcgtgggcg tgtctgggat tacgaggggt cccagttgtg ttgctgaggg ggcccaccct
      721 tcaatccggg catatcggga cactattggt ggcgggccat gatccctggg ttgcctattt
      781 gccgaacagg tttttcattg gggaacccca acgtgagctg gtaggaaatg tgtcgctacg
      841 ggacctgccg gcactgctag atcgcctgcg tgatgcagcc gatctgacta ttgttcgtgc
      901 agaccgattg tcagcggaaa aattcttcgg gaaggattat cttgcggttc ctgaatggat
      961 tgacatgcgt ctcgcccttc ctggggatct cgatgagttg gtgcgtagta accgaagtat
     1021 ccgggaggat atgcgtctgg tccgcaaaca taagttgcag ccattggtca ccgaagggga
     1081 ggagcgattt gacgagtttt atgactccat gtacgtccct ttctcgagag accgtcatgg
     1141 agccatggcc attatcaaag gccggcagga actacgtcgg tttatccgaa aagggggaat
     1201 cctctgggtt atgggagata atcagccact tgccggtgtt ctctatgaac gaaaacagga
     1261 cacatttaac cttttggcca taggcatggc ttcgggtgaa atccctctga aaaaaagagg
     1321 aattatggcc gcgctctact attacagcat ccagcatgcc cgccagttgg ggtttgctga
     1381 ggtgaatttt agagccgctc ggtcttccct gcatgacgga ttgcttcgct ataaacgcaa
     1441 atggggaaat gccctatttg acaatcctaa ttcctattat gatctgttga tccgttggaa
     1501 tagcgtaaac ggcatagtga aggacttctt ctctcacacg ggccttatct ttcgggacgg
     1561 agaacgcttg tctgcaatcc atgcggacga atcacagagt cgccgatctc tctggattga
     1621 agggctacat cgactctacc tcctgactga gtccggtcgc caaccgatga tagatgagga
     1681 agttctcgtt aagcgtagcc ggagccacac ccgattctca tcgacccttt catgatgctt
     1741 ggggaggcat cgagggccct atcaacattc ccgctttttg cgccgatgat cagtaagggt
     1801 agctgttgtg gcgagatgcg aggttcgaaa acctagcctt tggctagacg atagtttcta
     1861 actataggaa tgcgctgtat tcagtcgttg atttgaaagt catcaactag gaccacgacc
     1921 aaacgccctt cgccgaggtt tggctaagag gtacgcgaca cccaagagtg ggagtgccta
     1981 tgtgcggatt gtgcggcgtg gtgtattccg atcctaagag gccggtcgat cgggatatgc
     2041 ttcatcatat gacaaacatg atgtcccatc gtggaccgga tggggctggg tactttgttg
     2101 acccgggtat cggtctcggc ttccgtcgac tgagcatcat agacttggaa accggcgatc
     2161 agcccatttc caacgaggat aactctgtca ccgttgtatg taatggagaa atctacaact
     2221 acaaggaatt gcgtcaagat ctagaggctg cagatcatcg tttcaggaca cattcggatg
     2281 ccgaagtcct tgtccattta tacgaggatt atggggtgcg ttgtgtggat tttctgagag
     2341 gtatgttcgg cttcgctatc tgggattccc gtcatcgtcg tctcatgttg gcccgagatc
     2401 ggttcggtat caaacccctc agctatgcca tcaaacccgg tgctctgttt tttggatcgg
     2461 agttgaagtc catccttgca agcgggtgta ttgaccgtca ggttgatgcc ggtgctatga
     2521 aggagttgtt tgcggtcgga tttgttcaag cgccgaagac cctcttgtgt ccaatacggc
     2581 ggctcccacc cgcgcattat cttctccatg aaaacgagaa gatcacgatc cagcgttttt
     2641 gggatcactc ctttccatca aggggtgagg aaggctttca acgaagcgct caggaatggg
     2701 ccgagacggt acgagataaa cttgaagagt ccgtccgcct ccacctccga agcgatgtgc
     2761 cattaggttg ttatttgagc agtggaatcg attcaagtgc tatggcggga ttgatgagcc
     2821 gagaaatctc ggatcctatc cacacttttt ctgcggcgtt tgaagatccg ttgtttaatg
     2881 agataggtcc gaaaaggatt ttcacagatt tctcgggcta caatcttcgc catcacatca
     2941 caacctgcag aaccgccgat tttgatcggt tgccggaaat gatttggcat cgcgaagatc
     3001 ccaatatctc atctgagggc atcccccata tgttgctcgc cagaatggcg gctcagcagg
     3061 tgaaagtcgt cttgaccggt gagggctcgg atgaaccatt cctcaatctt ccactcagtg
     3121 tgcgtcaact catcgcaaac atcccgttcc tgaggaaaaa atattctcga tcaagtcggg
     3181 cattcgccgc ccacgccacg atggacctgt ctcgatacac ccaaataatc gataatgcct
     3241 cccaccgaag ttacgatgat cttttttcct atcaccttag caacatccat agttctttca
     3301 acgattcaga accgcatttc tttcttccga aggattttga aggaaggaat cgttttgctc
     3361 aattacagta tttggatatt aatgtgcgcc tttcggactt cattacaaga accctggatg
     3421 ctgcctccat ggcttatggc ctggaggcac gcgtgccatt cctcgatcat gaatttgtcg
     3481 aattatgcag ccagattcca tccgacttga aaatgcgagg gcttcaggaa aagcatattc
     3541 ttcgacgggc cctggaaggt gttttgcccg cggaaattct gaaaagaagg aagagggggc
     3601 tttccgcccc attctggcca tggcagggtc ttttgccgga atttgtcgca gacgctctgt
     3661 ccgaaagcag gctccacacc aaagggtatt tcaatcctca ttctgttcga cacatgcttc
     3721 agcaacatca aaacggcaag gcgaattttg gaagagaatt aatcggggtc ctcaatattc
     3781 aattatggga tgacctattt gtccaagggt gccgtccgtc gtgagatgca cgagtgtgtt
     3841 ggcctccaat cctgtgtcca cgctctattc catgcttcgc catccgcatg tcggacggga
     3901 gcagcttgtg gcctttcaaa acgagcgagt ttgccggttg gtcgcacatg cctatcgcca
     3961 tgtgccctat taccgtgggc tctttgatcg acatggactg aaaccccagg atatccgaac
     4021 ggtcgaggat ttgggggccg tgcccatcac ctccagaagt gatttgcaag gcttgccggt
     4081 cgatgaaatc atcgctcgga atgtggaccc gaggtctttg atcacccgcg cctccagcgg
     4141 gtcctccggg cgaccattta ttgtgcgtcg cacttggctg gaggaacgcc ttcatggggc
     4201 ctttcggtgg cgtgcgctcc atgcgttggg cctccgtgcg agcgacaaga tttgttatgt
     4261 gatggggaac tggaatatcc aacgacagga ccagctcgcc aagcgtatcc tggacaccct
     4321 cgggatggcc cggcgaaagg tcattgatgc cctgcagcca ccggaggaca ttgtccaggc
     4381 tctgcagaag ttcaaaccta cggtcgtggg ggggtatccc ggcgtgttgg ccaggatcgc
     4441 gcagactgtg aattgcgacg tactacggtc ccttcgactc cactttgtaa atcccggtgg
     4501 agaagtcctg acgccactca tgcgccaaca gattcaggac gggttttccg cgccagtcta
     4561 tgaaacgtat ggaagtatcg aattttacct tctggcctgg gaatgcccaa caaccgggga
     4621 atatcactca tgtgacgatg gcttaattat cgaagtgctg cgggatggga ctccggcgga
     4681 tgaaggagag atgggagagc ttgttggaac cgacttgcat tcgtttgcga tgccgctgat
     4741 ccgataccag cttggcgatg tcgtcacaaa gggaagccaa acctgtcgat gcggacaacc
     4801 gttttcgacg attcgatcga tccagggacg tatgatcgat tacttcgtgc ttcccggcga
     4861 cagggttgtc catccctatg agttgggggt cagaaaggtc ccctggattc gagagtttca
     4921 ggtgacccag gaacgcatgg aattcatcgt gatgaaagtc gtcccgtttc atacaccctc
     4981 cgccgaggag atggcagcgt tggttcaacc ggtgattgcg ttgctgggat ccgacgtgca
     5041 gttccaggta gtgttggtgg acacaatccc gagggaggca agcgggaaat ttcgagtata
     5101 tcgttcccat attcgatcgg cttatgacgc atttgcgtgg acggatcaaa aaatagttcc
     5161 tcctggagtc catgacgact gctaattcta atgccgatag tgcggtggat aaggagcaca
     5221 aggtgacttt tgcttgcacc ctatcatgaa aatttcaaga aacgcggata tctcaatcat
     5281 caagggatga cgatgtctcc acaggcaacg tattgaagag cctggtcatg atgcccgcat
     5341 aaaccgacat gaaggacaga ggggttgtct gcgaggcctc atcgggtgat gattacgtga
     5401 ggatcttttt cgatcgagcg gcgactctca ttcatatgga aacgtccgag gtgtttgctg
     5461 atctttgtga acgtttcagt cttgctgtgt tgcaacatat cgatgagaac gaacggttca
     5521 ttttggagcc gtattgagcg tcaggaagcg gagacacctt aacgattgcg gaggaaatgg
     5581 gatgctccgt tcgtccatcg gtcaccatta accgagatgc cctccacgta ttatggttga
     5641 aaatccaaac aaatgcacag gccatcattt tcggtttata gaacaggaac gaactattcg
     5701 ccggtgaggc cgcctttgtt tgctcgaggt tggtcatctg agtaatgagt ctccgggagt
     5761 tggaaatggg catggctaac caaagttttc cccgcttcaa cgactgggcg tggtggctct
     5821 atgagcatgc tcccgccggc ttggacgatg tagccaagag atgtgcccta tgggtcttgc
     5881 catgggttgc gcttcgcgta cctgtcgcta ttctgcgagg gccaaccaga cattccaagc
     5941 gattgggaat tatcgtagtg gctgggcttc acccctgggc cgattacctg cagcgtcgct
     6001 tctttgcatg tgcgcctcgt cgagaggtcg ttggagccgt gcctgtgtgg gcccttcctt
     6061 cgttcctaaa acgtttggct gttgatgcgg accttattgt cgcgcgcgtc gatcgtgtct
     6121 ctgccgcctc ttttttgaag acggttacct tgtggttccc gaaagcatcg gctgccgact
     6181 tttgctgccg gtcgattttg aaaagcttgc acaggccagc ctcagcgtaa aggaagattt
     6241 gggtacgctc cggcgtgagg gtttcacgat ggaggtctca catagggagg cagactgcga
     6301 ggcgttttac tcctcgatgt attttccctt cgtccagaag cggcacggag agttcgccgt
     6361 gatccataac gtgcatcagc tccgtcgaaa gtttcgtcgt gggggactca tctggctgcg
     6421 gcggggtgac catcgcattg ccggggcgct gtttgagcgg gagggt
//
LOCUS       DAORAQ010000144         6415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1798_length_6415_cov_63.321069, whole genome shotgun sequence.
ACCESSION   DAORAQ010000144 DAORAQ010000000
VERSION     DAORAQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1798_length_6415_cov_63.321069"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1073
                     /locus_tag="PKM72_11110"
     CDS             171..1073
                     /locus_tag="PKM72_11110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNP61384.1"
                     /translation="MNTQTEKIGFVGVGRMGANMARRLQDVGYPVTVVFDVDSERAKA
                     LAKELDCEAVSTPGEVAALSNTIFTVVTDDGAMREIFSESNQGSLLKNAEGRLFVNCA
                     TLSPQVHIDIEKQVEARGGKTLEACMASSIPQAREGTLYLMCGGKPEVFEQAKPILTT
                     LSAQLRLIGPAGQAAKVKALVNMVMNINTAGLAEGLGLGDALGLDLTMLREVFGQTGA
                     TSRVLETDGADMQNRDHECYFSVEHAAKDSGIALAMAQTQGLTLPLAKATLEQYQRLV
                     KLGKGELDKSGVAELTFKDRSSRH"
     gene            complement(1437..1895)
                     /locus_tag="PKM72_11115"
     CDS             complement(1437..1895)
                     /locus_tag="PKM72_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61385.1"
                     /translation="MAVKKAVKSTARQASKKASQVKAAAKKKSAAVAKQAKTAAKTGI
                     KKAKSATKEAKSTISQAKKTATKRAASVSSSAKKTVSQLKKKGAKTVASTTKTAQKKT
                     QKAKNTVSTAAKETGRTLGKTVGRNVGKAIKTVEQLVQQAQEYGKTLLSK"
     gene            2171..2773
                     /locus_tag="PKM72_11120"
     CDS             2171..2773
                     /locus_tag="PKM72_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNP61386.1"
                     /translation="MKIGVWIIHLALLSVGLSGCATFSSSPFSTNQEAQVTTGISFPQ
                     VVQDSSAHTGQIVKVGGVVLSAKRFMDRTEVMVLQIPLDNDSVPEWDRTESKGRFIAT
                     QKTFLDPATLPQGTRLTMIGEVTGQASRKVDEEEKVYPVLAIQALKVWPLIPRNGYGY
                     RPNWGPYPYWGGYWGSYWPYPPYAFGYVGRGYGGGKRFRR"
     gene            2868..3167
                     /locus_tag="PKM72_11125"
     CDS             2868..3167
                     /locus_tag="PKM72_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61387.1"
                     /translation="MKQVLSAVLSVLFVVGLAGVSSAGMKDTLKGAAEKPPETSADAH
                     AGMDMTEHAKEHAIEAQDATKKEAGGMMDQFKDGAKGKVNETQETANEKIDSIGK"
     gene            3233..3997
                     /locus_tag="PKM72_11130"
     CDS             3233..3997
                     /locus_tag="PKM72_11130"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNP61388.1"
                     /translation="MPVLLSLVFIAVVHFGLSGLSWAADAPVFGTFALYEVDKDWNDL
                     PMEERQKGGEEAKALLEAFKSQVTVDGYWTYGLTTESHFLLRLHSAELKANQQLLTQL
                     RSTGLGRHLDLEYTISGVTKELNYAPDFPDLLQQLKGAKYEGDPSTYGIMIPIRKNAE
                     WWNLSKEDRVALMKEHTIPTVAYLKSVKRKLYHSTGISDVDFLTIFETNNLVDFNDLV
                     IALRMVREDIFNVQLGEPTIIGTLKSWNDIVDLLVK"
     gene            4539..5699
                     /locus_tag="PKM72_11135"
     CDS             4539..5699
                     /locus_tag="PKM72_11135"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HNP61389.1"
                     /translation="MIQTLASLGLDSTVSLSIHRVLGKWTTVIVVLLCLLLGSMPHAI
                     AVEDSYIAGYAAAVLQHEFNATNASLNVKDGVVTVYAVSLGTLDRTKVQTALEAIPGV
                     TRVEIIEGIGDSEIPVTPAPDVISHKIPEPESKFLPDGLLFDPLHADPRWPHFSVAYR
                     DISKGSEPKTTGSANFGETFAIYRDAAPFNGQWEIAFQGGVFSVFDLDASSKDLVNAD
                     YTAGLLASYRTGSLSGFIRVHHQSSHLGDEFILNNQPNRINLSYEEFDLKLSYELFNW
                     FRVYGGGGILVNRDPSTLGRGTSQFGAELTSPWTLLSGKIRPVAYADFQVNERSNWTV
                     NRSLMAGLQFENARIGDRKLQLLMEYYKGASPNGQFYSQQTEWIGIGLHLYY"
     gene            5866..>6415
                     /locus_tag="PKM72_11140"
     CDS             5866..>6415
                     /locus_tag="PKM72_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNP61390.1"
                     /translation="MSIRNVDQSGLLMVKSKLDPNSNKNDLLALYRDMLLIRRFEEKS
                     AEMYALAKIAGFLHLYIGQEAVAVGCIHAIRPDDYVIASYRDHGHCLAKGSDPKKVMA
                     ELFGKATGLCKGKGGSMHLIDVERNFMGGYAIVGGHLPLAVGLAFASRYRKEDRVVLC
                     FFGDGAVPSGHTHEAFNLAALWK"
BASE COUNT         1513 a         1484 c         1714 g         1704 t
ORIGIN      
        1 tcaccaacag atctcgttca gggggctact aaggaggaat taaagaagca ccctaagaca
       61 caagaaaata ttctccctct gaaaacaccc cacagcgatt aagagaaggg gttttcgttt
      121 aaatgtttat gcgttttgca agaggctgct aggcaaggat ggaggaggac gtgaatacac
      181 aaactgaaaa aattggcttt gtgggggtgg ggcggatggg ggccaatatg gctcgccggc
      241 ttcaggatgt gggttatcct gtgaccgtgg ttttcgatgt ggacagcgaa cgggccaagg
      301 ctctggccaa agaattggat tgtgaggccg tgtccacacc gggtgaggtg gcagccttat
      361 cgaatacgat ctttaccgtg gtgaccgatg atggcgcgat gcgggaaatc ttttcagaaa
      421 gtaatcaggg aagtcttttg aagaatgccg agggacggtt gtttgtgaat tgtgcgacgc
      481 tgtccccgca ggttcatatt gatatcgaaa agcaagttga agctcgtggt gggaagactc
      541 tggaagcatg tatggccagc agcattcctc aagcgcgaga gggcacctta tacctcatgt
      601 gcgggggcaa gcctgaagtc tttgaacaag ccaaaccgat tttaaccacc ttaagcgctc
      661 agctacgtct cattggcccc gccggtcaag ccgctaaggt caaggccttg gtgaatatgg
      721 tgatgaatat caacaccgcc ggtttggcag aggggttggg actgggggat gcccttgggc
      781 tggatctgac gatgttacga gaagtctttg gccaaaccgg agcgacgtcg agagtgttgg
      841 aaaccgatgg ggccgatatg caaaatcgcg atcacgaatg ttatttttcg gtggaacatg
      901 ccgcaaagga ttccggcatt gccctggcca tggctcagac ccaaggtctg actctgcctc
      961 tggccaaagc gacgttggaa caatatcagc gattggtgaa gttggggaaa ggtgagttgg
     1021 ataaatccgg tgttgccgaa ctgaccttta aggaccgctc atcccggcat tagtattaaa
     1081 ctcgttttct cttcttccct ccgatcaaag aggctattgt catcccgatc tattcgggaa
     1141 tgctcgggac aaggtctccc gaaggatcac gctgagcggt agcattgtcc acctgggcac
     1201 agatgcttcg ccatgggctc agcatgacaa gttgctttgg gactacctga gtggaacgaa
     1261 ggatctggcg gagacgcgga atcgtcaacc ttggcacaga tccttcgccg tggtctcagg
     1321 atgaacggtc ggttgaaggg tatggcaagc acgcattgct tacccgctta ctcttcagga
     1381 tgaaatcaaa attttcctat ttaatcccac cccttcaagt tttgcgggac aaccggctat
     1441 ttgctcagga gggttttacc atattcctgg gcctgttgga ccagttgctc gaccgttttt
     1501 atggcttttc ccacatttct ccctacagtt ttccctaacg ttctcccggt ttccttggct
     1561 gcggtgctaa cggtgttttt cgctttttgg gtcttctttt gagcagtctt agtggtcgag
     1621 gccactgtct ttgcgccttt cttttttaat tggctcacgg ttttctttgc actcgaggaa
     1681 acgctggcag ctcgcttggt tgctgttttc ttggcttggg atatggtgct ttttgcctct
     1741 ttggttgcgc tcttggcttt tttgatcccg gttttggccg cggtcttggc ttgcttggcc
     1801 actgccgctg attttttctt tgccgccgct ttgacttgag aggctttctt ggatgcttgt
     1861 ctcgccgtgc tctttaccgc tttttttaca gccatcgtct gctcctttgg tgtatgtaga
     1921 ggtcattgga tagttatacg tgaaatgtaa cctggaacaa gggaaatcac aagttaaaga
     1981 accaaccgat cataggtgta tgcgattcgt gaatggaaca catgtgtcct gtgatttctc
     2041 ggtggcctct gaagaaacta aatgattcgg aatctcctca atcgggcaga aaagcttttc
     2101 atgccgtttt ttcctcttca aaggcccggg atagggggta taatgcgacc agggggtgag
     2161 agaggaatct atgaagattg gagtatggat cattcatctg gcgcttctgt cggttggcct
     2221 gagtgggtgc gcgacatttt cctcatcccc tttttccacc aaccaggaag cgcaggtgac
     2281 gaccggcata tcatttcccc aggtggttca agattcctcc gcgcatacag gccagattgt
     2341 gaaggtgggc ggggtggtgt tatccgcgaa acgtttcatg gaccggaccg aagtgatggt
     2401 gcttcaaatc cctctagaca acgattctgt tccagaatgg gaccggacgg aatccaaagg
     2461 ccgtttcatt gccactcaga agacttttct tgacccggcc acgcttcccc aaggcactcg
     2521 cttaacaatg ataggagaag tcaccgggca ggcctcaagg aaagtggatg aagaagaaaa
     2581 agtctatcct gttcttgcca ttcaggcgtt gaaagtgtgg ccactgattc cccgaaacgg
     2641 gtatgggtat cgtcccaatt gggggcctta tccatattgg ggtggttatt ggggttcgta
     2701 ttggccttat ccgccttatg cttttgggta tgttggtcga gggtatggtg gcgggaaacg
     2761 gtttcgtcga tagacatggt ccatatccca gctaattgac ttcagtttga ggaaaggtgc
     2821 agagtaattt ttccctctca ttcacgttct tccttaagga ggtttttatg aagcaggtac
     2881 tatctgcagt cctttccgtg ttgtttgtgg tgggtttagc cggagtgtcc tccgcaggaa
     2941 tgaaggatac cttaaagggt gcagccgaaa agccaccgga gacttcagct gatgctcatg
     3001 cgggcatgga catgacggaa catgcaaaag agcatgcgat tgaagcccag gatgccacga
     3061 aaaaagaagc cggtggcatg atggatcaat tcaaggacgg ggccaaggga aaagtaaatg
     3121 aaacgcagga aacggctaac gaaaagattg acagtattgg taaataagac gccttcctaa
     3181 gttggtcttg ggaatgttta aaagtaaaac tcaggaaaga aggagtgaaa atatgcccgt
     3241 attactatcc ttggtgttca ttgcggtcgt gcattttggt ttgtcgggtt taagctgggc
     3301 tgccgatgcc cctgtttttg ggacgtttgc cctttatgag gtggataaag attggaatga
     3361 tttgccgatg gaggaaagac agaaaggcgg ggaggaggcc aaagctctgc tggaggcatt
     3421 caaaagtcag gtgaccgtgg atggatattg gacctatgga ttgacgacag aatcccattt
     3481 tttgcttcgg ctgcattccg ctgaactcaa agccaaccag caattactga cccaacttcg
     3541 gagtaccggc cttggccggc atttggattt ggagtacacg atcagtgggg tgaccaagga
     3601 gctgaattat gctccggatt ttcctgattt gcttcaacaa ctgaaagggg cgaaatacga
     3661 gggtgatccg tctacctatg gaattatgat ccccattcgc aaaaatgccg aatggtggaa
     3721 cctgtccaaa gaggatcgtg tggcgctgat gaaagaacac accattccca ccgttgctta
     3781 tttaaaatcg gtgaagcgga aattgtatca ttccaccggg atctcggatg tggattttct
     3841 caccatattt gaaaccaata atctggtgga tttcaatgat ctggttattg cattgcggat
     3901 ggttcgtgaa gatatcttca atgttcaact cggagagccg acgatcatcg gcacattgaa
     3961 aagctggaat gatattgttg accttcttgt gaaatagccg gatgacggcc tggtgatggg
     4021 cccgtcatcc ttggggcaat caatcttggc atcagaggtg aaaggatttg tacatgccaa
     4081 aatggggaaa atggaaaagc aatccatgaa agaggaaccg acgaaaaaag aaccgatgaa
     4141 aaagtaaccc tcattccttt tatgcatctc taagaaggcc ggctgtggac tgactctgca
     4201 gccggccttc ttattggaaa caaataggac caaccacgtc actgttgaat aaaaataatc
     4261 agtcccctgc acgtgaaatc cggtgtttta cttatttgac aatgtccaaa ttacgcccct
     4321 cgccatgacc ttttgtctgt ccaaccaaac acaaacttcc tgttattttg tgttcagggc
     4381 ttcttcactt tagaccagcc gataagcccc aacctgaccg ctcgaaaagg gtggttgccg
     4441 tgtcagcact gagaactatc gggtatttct ccaagcgttg gttatgtttt agccaggttc
     4501 atcataccac catgtttcgc caaggagagc agattccaat gattcaaacg cttgcttccc
     4561 tcgggctgga ttctaccgtg tccctaagta tccatagagt attagggaaa tggacaacag
     4621 tgatcgtggt cctgctctgt cttctcttgg gctcgatgcc tcacgctatc gcggtggaag
     4681 attcttacat cgccggctat gctgccgcag ttcttcaaca tgagttcaat gccaccaatg
     4741 cctccctgaa cgtgaaggat ggtgtcgtga ccgtctatgc cgtttcgctc ggcacccttg
     4801 accgaacgaa ggtacagacc gctttagaag ccattccggg tgtgacacgt gtggaaatca
     4861 tagagggcat tggcgactcc gagattcccg taactccggc gccggatgtc atttcccata
     4921 aaattcccga gcccgaatcc aaatttcttc ccgatggcct tcttttcgat ccgcttcatg
     4981 ccgacccgcg ttggccgcat ttctccgtgg cctaccggga tatttcaaaa ggttccgaac
     5041 ccaaaacaac cggctcggca aattttggag aaacgttcgc gatttatcgc gatgctgcgc
     5101 cattcaatgg ccaatgggaa atcgccttcc agggcggagt ctttagcgta ttcgatttgg
     5161 acgcgtcgtc caaggacctt gtaaacgcgg attatacagc cggccttctt gccagctatc
     5221 ggaccggttc gctttccggc ttcattcgcg tccatcatca aagttcacat cttggtgatg
     5281 aattcattct caataaccag ccgaacagaa tcaatttgtc ctatgaagaa ttcgacctga
     5341 agttgtccta tgaactattc aactggtttc gtgtgtatgg cggcggcggg attctggtaa
     5401 atcgggatcc cagcacgctc gggcgcggca ccagccaatt cggggccgaa ctgacaagcc
     5461 cttggacgtt gttgagcggg aagattcgcc cggtggcgta tgcagatttc caggtcaacg
     5521 agagaagtaa ttggacagtc aaccgttcgc tcatggctgg gcttcaattc gaaaatgccc
     5581 gcatcggcga ccgcaaactc caattattaa tggagtatta caagggggct tcacccaacg
     5641 gtcaattcta ttctcagcaa accgagtgga tcggaattgg tcttcatctg tattactgac
     5701 accaagcctg gcctaccttg agagccatcc cggcgcacat gcttttgggc tacggatggt
     5761 aaacatgaaa ttgcggtaaa gaccttaaga attgtatcgg tatgcgatct cttgctacag
     5821 ttcttctaag ttcttctaac gttccagaga cagaacctaa ggggaatgtc gatacgcaat
     5881 gttgatcaat ccgggcttct catggtgaag agtaaattgg atcctaactc gaacaagaac
     5941 gatcttttgg cgttgtatcg cgatatgctt ctcattcgac gctttgagga gaagtccgcc
     6001 gagatgtacg ccttagcgaa aattgccggc ttccttcatt tatacatcgg ccaggaagcg
     6061 gtcgccgtgg gatgtatcca tgccattcgc ccggatgact atgtcattgc ctcctatcgg
     6121 gaccacggac attgtctggc caagggctcg gatcctaaaa aggtgatggc tgaattgttt
     6181 ggaaaagcca caggcctgtg caagggcaaa ggtggttcca tgcatctcat cgacgtcgaa
     6241 cgcaatttca tgggcgggta tgccatcgtc ggtgggcatt tgccgcttgc tgtcgggctg
     6301 gcgtttgctt cacgttatcg caaggaagac cgggtggttc tctgtttttt tggtgatggg
     6361 gcggttccca gtgggcatac ccatgaggcc tttaatctgg ctgcgttatg gaaat
//
LOCUS       DAORAQ010000145         6402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1804_length_6402_cov_70.252718, whole genome shotgun sequence.
ACCESSION   DAORAQ010000145 DAORAQ010000000
VERSION     DAORAQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1804_length_6402_cov_70.252718"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..682)
                     /locus_tag="PKM72_11145"
     CDS             complement(182..682)
                     /locus_tag="PKM72_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61391.1"
                     /translation="MPLEKEDGAKVLQLITSRYNDREWRKKIEKTLSLPPSGLKDEDQ
                     RKAFLYLKVGLQMYKSRRADAPSWIVGGFATKEVIDRALFKPTVIDPDFTKDQVAGFG
                     EDPGSEVDAVWWEDMLVNWFEEPEEEGDDDTEAESSEEGELSAEAANASDDSTKSPSH
                     AKKPTT"
     gene            complement(775..2145)
                     /locus_tag="PKM72_11150"
     CDS             complement(775..2145)
                     /locus_tag="PKM72_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNP61392.1"
                     /translation="MSNLSTIHGDRFPELADTVLKRAKALGATEADLLVAEGDSVSVQ
                     VRMSEVDRLSKAREKVLGIRVFFGKRSASSSTSDFSKASLDRLVSDTCSLARAVVEDE
                     TSGLPAPDHMVTDIPDMDMKDDRQLTVDEEIDLARRTEQSAFSADSRITNSEGAECSA
                     GYGSILLANSHGFIGSYASSSYSLSVSPIALDSQNGGMQRDYWYSVKRKFHELESPEK
                     IGQEAARRTLRRLGSRTITTQEVPVIFDPETAKGLLGHISSAVSGYSLYKGASFLLGQ
                     LGKSIASDLVTVIDDGRLAGGLGSRPFDGEGLPTRKTLVVDKGVLSSYMLDTYSGRKL
                     GMASTGNASRSVGENPTVGATNLYLSPGSYSPEEILKSMKRGLFVTDLIGFGVNLVTG
                     DYSRGAVGFWVENGELTHPVEEVTIAGNLQQILKDIEMVGNDLEFRGRLASPTVKIRK
                     MMVAGH"
     gene            complement(2183..3610)
                     /gene="tldD"
                     /locus_tag="PKM72_11155"
     CDS             complement(2183..3610)
                     /gene="tldD"
                     /locus_tag="PKM72_11155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNP61393.1"
                     /translation="MVPSLSDFALQEKDVLQALNKAIARDVDYVDMYVESCVTDSVSM
                     EESLVKRAVKSISQGAGIRAVAGERTGFAYSDDLSVRDLEIAADTARYIADSPQGDNP
                     IAVTHRSQPTQNLYPLSQPLTDITTEARVDLLNKIDVEARRYDPRITKVMASFNTEQK
                     IFLVINSQGQLVGDVQPLSRLQITCIAEDGSNRQVGSFGGGGRVGFTFYLEQNRAMEF
                     AREAARQAILNLGAVEAPAGTMPVVLGGGWPGILLHEAIGHGLEADFNRRKTSAFSDL
                     VGKSVASELCTIVDDGTLPFRRGSMNIDDEGTPTSRTMLIEKGILRGYITDRLNARLM
                     GIPLTGNGRREDFRSIVLPRMTNTFMLAGESDPQDIIRSVKNGLYAVSFGGGQVDITS
                     GKFVFSASEAYLIEDGKVTKPVKGATLIGNGPDVLKKVSMVGHDMKLDEGIGTCGKDG
                     QSVPVGVGLPTIKIDEMIVGGTAMG"
     gene            3849..4616
                     /locus_tag="PKM72_11160"
     CDS             3849..4616
                     /locus_tag="PKM72_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNP61394.1"
                     /translation="MDARQVAEGELLDQLDLTSLPRHIAIIMDGNGRWAAQRGLPRIA
                     GHKEGLRALQDVLEIGHELRIPYITIYAFSQENWKRPHSEIRLLMGLLEQYLNQERQR
                     FQERQVRFLPIGRLEQLPPSVRSLALEVAEETRHLTQRTLAVALSYGGRTEIVDAARK
                     VAIEVQMGSLTPDQIDESLFEQYLSTWGLPDPDLLIRTSGEARISNFLPWQIAYTELY
                     FTKTLWPDFRRAETLLALLDYQKRERRFGRITQTVSP"
     gene            4697..5506
                     /locus_tag="PKM72_11165"
     CDS             4697..5506
                     /locus_tag="PKM72_11165"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNP61395.1"
                     /translation="MDPRRLYSAIVFIPLLYAGIRYSPPWLLSILIGGASLLALWEFL
                     TLYFGRTGTLIPKVISCLCAVMLLVAMYTGYSLALNPWFLGIVGVILTGFFISPTTMR
                     QRLPLWVAYPFGILYVVVLLGHFILLRQLPHGIALIFFVLAITWLADTGGFVVGLSLG
                     RHALAPTLSPKKTIEGLFGGILFSVLGALISHFWFLPFFSLGECAILGMGMAIIGTLG
                     DLAESAIKRSVSVKDSGTIIPGHGGVLDRVDSLLFTAPVIYYYALFTGSNS"
     gene            5540..>6402
                     /gene="dxr"
                     /locus_tag="PKM72_11170"
     CDS             5540..>6402
                     /gene="dxr"
                     /locus_tag="PKM72_11170"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNP61396.1"
                     /translation="MKHIVILGSTGSIGNSTLDVISRFPEEFQVVGLAAGSNDQALEE
                     QIRRFKPDVAALFCQDAANRLRARMGRTSTEILDGEQGLCAVASASKGDLVISAIVGG
                     AGLKPTLTAIQAGRQVALANKEPMVMAGRLMQLEAHKHHVTIFPIDSEHSAIFQSMEG
                     HRKVDIRRIVLTASGGPFWDWPINDLEHVTPEQAMQHPNWKMGAKITTDSATLMNKGL
                     EVIEARWLFDIPQDQIDVVIHRESIIHSLVEYCDGSVIAQLGHPDMRTPISYALKYPE
                     RIPLNPPLLDLG"
BASE COUNT         1545 a         1754 c         1472 g         1631 t
ORIGIN      
        1 agccgacaaa cccaggagtt taatcgatgc aaggaagaat agacgaagcc cttgtgtgtc
       61 gctgaaactt tcaacgatga agaggaaaaa ggccggtgag tcggtataga agtatgtccc
      121 tccatgccct tactgttgaa aataggcaca agagacctgc gagaagcctg acaaaaataa
      181 cttaggtagt gggttttttt gcgtgagatg gactttttgt ggagtcgtca gaggcattag
      241 cggcttcagc agatagttct ccttcctcag atgattcagc ctcggtgtcg tcatcgccct
      301 cttcttccgg ctcttcaaac caattgacga gcatgtcctc ccaccagacc gcatcgactt
      361 ccgaacccgg atcttctcca aagccagcca cctggtcttt ggtaaaatcg ggatcaataa
      421 ccgtgggctt aaacaatgcc cggtcaatca cttccttcgt ggcaaaacct cccacaatcc
      481 aagaaggagc atccgccctg cgggatttgt acatttgcag cccgacctta agatagagaa
      541 atgctttgcg ttgatcttcg tctttcaacc ccgatggggg taagcttaag gtcttttcaa
      601 ttttttttct ccattcccgg tcattgtacc gggaggtgat caactggaga actttggctc
      661 catcctcttt ttccagtggc atcctaactc cttatgggcc tgcggccgtt ttctgtcttc
      721 atgaaatgga taggtatcga tagacccttg aatgagtcct tgtcgtgtat ttctctaatg
      781 accggcaacc atcattttcc gaattttgac ggtaggactg gccaaccggc cacgaaattc
      841 taaatcattc ccgaccattt caatatcctt caagatctgt tgtaaattcc cggcaatggt
      901 gacctcctcc acaggatggg tcagttctcc attttcaacc caaaacccta ctgctcccct
      961 tgaatagtct ccggtgacaa ggtttacgcc aaacccaatc agatccgtaa cgaataaccc
     1021 ccgcttcatt gacttgagaa tttcttccgg tgaataggag cctggcgaga gatataaatt
     1081 cgtggcaccc actgtcggat tttcacccac ggatcgtgac gcattgccgg tggatgccat
     1141 tcccagtttt cgtccagaat acgtatctaa catgtaactc gacagcacgc ctttatcgac
     1201 caccagggtt tttctcgtgg gtaacccttc accatcaaaa ggtcgagacc caagccctcc
     1261 ggctaatcga ccatcatcga tcacggtcac taaatctgag gcaatagact ttccgagttg
     1321 tcccaacaga aatgaggccc ccttatacag ggagtagcct gaaaccgctg aggaaatatg
     1381 ccctaggagg cctttggccg tttccggatc aaagatcacg ggaacttctt gcgtcgtaat
     1441 ggttcgactg cctagccgcc gcagggttcg gcgagctgcc tcctgtccga tcttttccgg
     1501 gctctctaat tcatgaaatt tccgtttcac ggaataccaa taatcccgct gcatgccgcc
     1561 attctggctg tccaatgcga tgggcgacac agagagtgag taagaggaac tggcgtacga
     1621 tccaataaat ccatgggaat tcgccaacaa aatcgaccca taaccagccg aacactcagc
     1681 cccttcagaa ttggtgattc gggaatcagc ggaaaacgcg gattgttcgg ttctcctggc
     1741 caggtctatt tcctcatcaa ctgtcaattg ccgatcatct ttcatatcca tgtcggggat
     1801 atcggttacc atgtgatccg gcgccggcag tcctgacgtt tcgtcttcaa ccacagccct
     1861 ggccaaacta catgtgtcac tgactaaacg atccaacgag gctttggaaa aatccgatgt
     1921 tgaagaactg gctgaacgtt tgccaaaaaa cacccgaatc cccaagacct tctcacgggc
     1981 tttggagagg cggtccactt ctgacatccg cacctgcacc gagactgaat caccttcagc
     2041 cacaagaaga tccgcttctg tcgcccccaa ggccttggct cgcttgagaa ccgtgtccgc
     2101 caattccggg aaacggtcac catgtatggt tgacagattt gacattatta aatggagaaa
     2161 aacaaaacgg aagggttatc catcagccca tcgccgtgcc accgacgatc atttcatcga
     2221 ttttaatggt aggaagtccc acgccgaccg gaaccgattg cccatctttt ccgcaggtgc
     2281 cgattccttc atccaatttc atatcatgtc cgaccatcga aactttcttg agaacatcag
     2341 gaccgttccc aatcaacgtt gcgcctttga ccggtttggt caccttccca tcttcgatca
     2401 gataagcttc gctggctgaa aagacgaatt ttccactggt gatatcaacc tgtcctcccc
     2461 caaacgagac ggcatacaac ccgttcttca ccgaccggat gatatcctgc ggatcggatt
     2521 ctccggctag catgaaggta ttggtcatcc gtggaagaac aatgcttctg aaatcttccc
     2581 gtcttccatt ccctgttaac gggatgccca tcaagcgggc attgagccgg tccgtgatat
     2641 agccgcgcaa aatacctttc tcaatgagca tggttcgtga cgtcggcgtc ccctcatcat
     2701 cgatgttcat agacccacgc cggaatggga gcgtcccatc atccacgatg gtgcataatt
     2761 cggatgcgac ggactttccg actaagtcac taaaggctga ggttttgcga cgattgaaat
     2821 ccgcttccaa cccatgcccg atcgcttcgt gaagcaaaat gccaggccag cccccaccta
     2881 agacaaccgg catggtgcca gccggagctt cgaccgcgcc tagattaaga atcgcctggc
     2941 gtgcagcttc tcgcgcaaac tccatcgcgc ggttctgctc caggtagaaa gtaaatccga
     3001 cccgtccccc tcctccaaat gacccaacct ggcgattgga tccatcttca gcaatgcagg
     3061 taatctgaag acgagaaaga ggctgcacat ctcccaccag ctgcccttgg gagttaatga
     3121 ctaaaaatat tttctgttcc gtgttaaaag acgccatgac cttggttatt cgcggatcat
     3181 aacgcctggc ttccacgtct attttattca gtaagtcaac ccgggcttcg gttgtaatat
     3241 cggtgagcgg ctgagacaac gggtagagat tctgtgttgg ttgagaacga tgggtgacgg
     3301 ctatagggtt gtccccttga ggagaatccg caatatatcg tgccgtatca gcggcaattt
     3361 caagatctcg cacagacaga tcatctgaat acgcaaaccc tgttcgttca ccagccaccg
     3421 ctcgaatgcc tgccccctgg gaaatacttt tgaccgcgcg cttgaccagg ctttcttcca
     3481 tagaaacgga atcggtcacg caggactcga catacatgtc cacgtaatcc acatcgcgag
     3541 cgatggcctt atttaaggcc tggaggacat ctttttcttg gagggcaaaa tctgaaaggc
     3601 tgggtaccat aaacctcaat tacaacaaat tacgtatcaa agaaggaaaa actggagtat
     3661 agcccatctc ctaggtgggc gcaatttccg gtgaaagttc tgcccgcaat ttgacaagaa
     3721 tcggtttcat tattagactg aaattgttgt gaaattttgc tagtttctaa aacctttcaa
     3781 ctgaattgca acgcggttct caccctcaca atcggtttca acacaattca atggatgact
     3841 ttcacgcggt ggatgcaaga caagttgctg aaggcgaatt gctggatcaa cttgacctca
     3901 cctcccttcc ccgacatatc gcgatcatca tggatggcaa tggccgatgg gccgctcaac
     3961 gagggttgcc tcgaattgcc ggtcacaaag aagggcttcg tgcgctccag gacgttctgg
     4021 aaatcgggca tgaactcagg attccctata tcaccatcta tgcattttcc caggaaaatt
     4081 ggaaacgtcc tcattctgag attcgactcc tcatggggtt gcttgagcag tacctcaatc
     4141 aagaacgcca acggtttcaa gagcgacagg tacgcttcct gccgatagga cgacttgaac
     4201 aactgccgcc ttccgtccgg tctctggcct tagaagtagc ggaagaaacc cgccatttaa
     4261 ctcaacgcac tctggccgtc gccctgagct atggaggacg caccgaaatt gtcgatgccg
     4321 ctcgaaaagt tgcgatcgag gttcagatgg gatcgctcac accagaccaa attgatgagt
     4381 ccttatttga gcaatatctc agcacatggg gtctccctga tcccgatttg ctgattcgca
     4441 cttctggaga agcgcgaatc agcaacttcc ttccctggca gattgcctac acggaactct
     4501 atttcaccaa aaccctgtgg cctgattttc gtcgcgctga gactcttctt gccttactcg
     4561 attatcaaaa acgggagcgt cgttttggcc gaatcactca aaccgtttcg ccataacacg
     4621 ggctgtggcc atcccgcatt cctaataccc tacttcttgt tcatctaacc cactgctaat
     4681 tgtactcccc taaacaatgg atccccggag actctactca gccatcgtat ttattccttt
     4741 gctctatgcg ggaatacgct attctccacc ctggctgctc tccatcctga ttgggggcgc
     4801 ttccctcctt gcattgtggg aatttctcac gctctacttt ggacggaccg gcacccttat
     4861 tcccaaggtg atctcctgcc tttgtgcggt catgctcctt gtcgctatgt ataccggcta
     4921 ctccctcgcc ttgaatcctt ggttcttggg aatcgttggg gtcattttga caggattttt
     4981 catatctccg accaccatga gacaacgtct ccccttatgg gtggcctatc cctttgggat
     5041 cctctatgtt gtggttctgc tcgggcattt catactcctc cggcaactcc ctcatggaat
     5101 cgccttaatc ttttttgtct tagccatcac gtggttggct gataccggtg gatttgtcgt
     5161 aggtttatct cttggacgac atgccctggc tccaaccctg agtcctaaaa aaacgattga
     5221 aggcctgttc ggagggattc tcttttccgt actgggagct ctaattagcc acttttggtt
     5281 tctccccttt ttctcgctgg gggaatgcgc tattctcggc atgggcatgg ccataatcgg
     5341 aacccttggt gatcttgcag aatccgcgat caaacgtagc gtgagtgtca aggactcagg
     5401 cacaatcatt ccaggacatg ggggagtgtt agatcgtgtg gatagcctgc tctttaccgc
     5461 cccggtcatt tattactatg ccttattcac gggatcaaac tcataaccag accgtacgca
     5521 accataggga gagaatctgg tgaaacacat cgtcatttta ggatctaccg ggtcgattgg
     5581 gaacagtacc ttagatgtga tctccagatt ccctgaagag tttcaagtcg tcggcctggc
     5641 cgccgggtca aatgatcagg cccttgagga acaaatccgc cgcttcaagc ccgacgtggc
     5701 tgcgcttttc tgccaggatg ccgccaaccg attacgggca cggatgggac gcacgtcaac
     5761 tgaaattttg gacggagaac aggggctctg cgccgtcgcc agtgcatcga aaggcgattt
     5821 ggtgatttct gctatcgtcg gaggagcagg gctcaaacca acattgacag ccattcaagc
     5881 aggccggcag gttgccctcg ctaataaaga gccaatggtc atggcaggtc gattaatgca
     5941 actggaagcc cacaaacatc acgtgacgat tttccccatc gatagcgaac atagtgctat
     6001 tttccaatca atggaagggc accggaaggt cgacattcgc cgaattgtgc taacggcttc
     6061 cggcgggcca ttttgggatt ggcccatcaa tgacctcgaa catgtgaccc ccgaacaggc
     6121 gatgcaacac cctaattgga aaatgggtgc taaaattacc accgattccg ccaccttgat
     6181 gaataaaggc ctggaggtga ttgaagcccg atggctcttt gatatccctc aggatcaaat
     6241 tgatgtcgtc attcaccgag aaagtattat ccactctttg gtagaatatt gtgatgggtc
     6301 agtcattgcg caattaggcc atccggatat gcgaacacca atctcctatg ctctcaaata
     6361 cccggaacgc attcctttga atcctccact cctggattta gg
//
LOCUS       DAORAQ010000146         6397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1806_length_6397_cov_62.975244, whole genome shotgun sequence.
ACCESSION   DAORAQ010000146 DAORAQ010000000
VERSION     DAORAQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1806_length_6397_cov_62.975244"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..693)
                     /locus_tag="PKM72_11175"
     CDS             complement(46..693)
                     /locus_tag="PKM72_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP61397.1"
                     /translation="MVTKPRVILADDHSLVLEGLAKLVEEDCDLVGKVEDGRALLQAA
                     QKLEPDVIVLDISMPKLNGLDAGRQLKKLFPTIKLIFLTMHADPLYAKEAFQIGASGF
                     LLKRSAASELMLAIHAVMKGQFYVTPAIAKDFLGTFSQEMSFPQTETDLLTPRQREVL
                     QLIAEGHTNKEIATILNVSAKTIEFHRTQIIRELNLHSTAELTRYAIVHGLVSPE"
     gene            complement(783..1139)
                     /locus_tag="PKM72_11180"
     CDS             complement(783..1139)
                     /locus_tag="PKM72_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61398.1"
                     /translation="MAQSNKPPKSKRRTEQSGKGGPFIKKATLGRPVGVQSPGPPTHP
                     RLSSRDKKSSRSSSDVKKPQDGLPVEGIGVSPAGVQPFPEMQNEELCRTRLELEVARD
                     RYAVFSGGDGFPECQE"
     gene            1956..2399
                     /locus_tag="PKM72_11185"
     CDS             1956..2399
                     /locus_tag="PKM72_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP61399.1"
                     /translation="MNHLLKLALRNGSLALLVIGMGMSSGWAQEQDVIAGGKQLFQKF
                     CASCHGSQARGNGPLSESLKPKPANLTRLSANHRGSFPFWQAYRMIDGRDHIPSHGTR
                     DMPVFGIWFRIPDDEVSIETEWADQVRGRIWQLLSYLESIQEPEK"
     gene            2469..2873
                     /locus_tag="PKM72_11190"
     CDS             2469..2873
                     /locus_tag="PKM72_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP61400.1"
                     /translation="MMYQPHKHIGLLSFILIMALPILGLSQEVPGNPQNGKVLFEKHC
                     ANCHGKKGTGDGPDTKFLLVQPANFHSLESRSKTDWELMNVITYGAIFSPMHGWSERL
                     TEDERWDVLRYIRFLAPFNPIANLYPLPETHG"
     gene            3325..3759
                     /locus_tag="PKM72_11195"
     CDS             3325..3759
                     /locus_tag="PKM72_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNP61401.1"
                     /translation="MDGLESSTRWEHFPHEADMGIRGFGITKAQAFEQAALALTAVVT
                     DPQRVAAQMEQHFTCEAPDDELLLVDWLNALIFEMACGHMVFAWFDVTITDRHLEGIA
                     YGEPVDRMKHEPAVEIKGATYTELFVRRIEGNGWVAQCVVDV"
     gene            3912..5273
                     /locus_tag="PKM72_11200"
     CDS             3912..5273
                     /locus_tag="PKM72_11200"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNP61402.1"
                     /translation="MPGIIYADESLVRDMDLKVYEQVVNVATLPGIVQASFAMPDAHW
                     GYGFPIGGVAAFDPQQGGVVSAGGVGFDISCGVRTLYTGLRADDITPLKEKLANLLFA
                     KVPAGVGSTGQLRLKPKEMDDMLAGGAQWAVQRGYGTANDLSRIEEHGCMTGAKPGCV
                     SDHAKKRQQDEMGTLGSGNHYLEIQEVVETYHDPLSEKLGIQAGDILVSIHCGSRGLG
                     HQIGTEYLKKMVVTASQYGITLPDRELACAPIDSPLGQEYLGAMRAAMNCALANREII
                     THLVRQAFVEIFPQAALPLLYDVSHNTCKVEEHQIGGRSRRVFVHRKGATRAFGPGHA
                     DIPPDLQPFGQPVLIGGTMGTASYLLVGTKEGMGLAFGSACHGAGRSMSRHQATRQWK
                     GHRVIQDLAAQGILIKSPSARGVAEEAPGAYKNVTAVVDAAEQAHLARKVAKMRPLIC
                     IKG"
     gene            5348..5731
                     /locus_tag="PKM72_11205"
     CDS             5348..5731
                     /locus_tag="PKM72_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61403.1"
                     /translation="MDHAQREQSLLKVLEAFTKGLTSLKEAIALVHSANDQLPPGEQR
                     KTIQKKIEGAERMIQVGHAAMGQSLGFQLCHCTWPPQVMISSHYSNETKVERFTCPNC
                     GKTLSLHGRQAPGEALTAFDPYDVY"
     gene            5889..6397
                     /locus_tag="PKM72_11210"
                     /pseudo
     CDS             5889..6397
                     /locus_tag="PKM72_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
BASE COUNT         1628 a         1633 c         1618 g         1518 t
ORIGIN      
        1 gttctacgat aaaacatccc tggcttggaa aatccgaaga atggcttatt caggtgaaac
       61 gaggccatgg acaatcgcat acctcgttaa ctccgccgtg ctatgaagat ttaactcccg
      121 gattatctgg gttcgatgaa attcaatggt tttggcagac acattcaaga tcgtcgcaat
      181 ttctttgttg gtgtggccct cggcgatgag ctgtagcact tcccgttgtc ggggcgtcaa
      241 caaatccgtc tcggtctggg gaaacgacat ttcttgactg aacgtaccca gaaaatcttt
      301 ggcaatggcc ggcgtcacat aaaactgtcc tttcatgacc gcatggatcg cgagcatcaa
      361 ttcggaagcc gcagaccgtt ttaagagaaa cccggaagcc ccgatttgaa aggcttcctt
      421 cgcatagaga ggatccgcat gcatggtcag aaaaatcaac ttaatcgttg gaaataattt
      481 cttcaattgg cggcccgcat ccagtccatt taattttggc atggaaatat ctaagacaat
      541 cacatccggt tccagctttt gagcagcctg aaggagggcc cgcccatcct ccactttccc
      601 cacaagatca caatcctcct caaccagttt agccaggcct tccaagacca aggaatggtc
      661 atcagctaat atgacacgag gtttggttac catacccaat gtcctctgct gcaatacctt
      721 tgacgtactc gaaaagacat tcccaactcc tttttcttca tgcgttattc cctcccctac
      781 tgtcattctt gacactctgg aaatccatcc ccgccgctga atacggcata acgatcacgt
      841 gcgacttcaa gctcgagtcg agtccggcat aattcctcat tttgcatctc cggaaaaggt
      901 tgaacccctg ccggcgatac ccctattccc tcgacgggta aaccatcctg aggttttttg
      961 acatcagaag aagaacgaga agatttttta tccctggagg aaagcctggg atgtgttggt
     1021 gggcctgggg attgcacccc gactggacgc ccgagagtgg ctttcttgat gaaagggccg
     1081 cctttcccgg actgttcagt tcgcctcttg gattttgggg gcttatttga ctgagccatg
     1141 aaagcttttc cctaaggggt cagttttcaa gcacctcaca gggcccaatc atattcattt
     1201 ttccctgaaa ggtacagctc tgcagttatc ccccttcatt aggcctgcct tattaatgaa
     1261 ttgcattggg catccgtacg cgaatttgct tgaatggata tccccactcc cgctacgagg
     1321 atgatggaat cgaaagatgc aaccccctgt gaggaagact gggacgtcca attctagagg
     1381 tgtggggaaa gatgctacac ttcctttggc tatccttatg aatatttccc ttctttcctc
     1441 acttttcctc aagcagatcc agatccacta accaattttt tcacttattc ttcctcacgg
     1501 ccttcaacgt ttctccacct tctcaacgaa atacaattcc ctttctccaa tccactcttt
     1561 ttgaaacgta ttcttttaga ccataaatca gtacctggca caaagtttga tactaccttc
     1621 tcaatcaggg gggaaccaaa acgtcatttt gccaaaaaat tgtaaattca tagacacaat
     1681 tgaaatacgc cactcattta ttgctttaac aacacgctgc ctgcaggagg tgactcaatg
     1741 gaatgtcctc gctgtcatgg tttaatggtc attgatgcct gtttgaatct ggaaaatgtt
     1801 catcacactg tctggattca tgaatggcga tgtttgaatt gtggagaaat ctctgatccg
     1861 caaactttgg caaatcgcgc tccacgccaa caggatgtca gcaaccgact ccccaagagg
     1921 ttgggtgaat gtttcagtca cgcaaaggat acccaatgaa tcatctcctt aaacttgccc
     1981 tgcggaacgg gagccttgca ttgcttgtta taggcatggg aatgagttcg ggatgggcac
     2041 aggaacagga tgtcatagcc ggaggcaaac agctctttca aaaattttgc gcatcctgcc
     2101 atggaagtca ggccagagga aatggccctc tttctgagtc tttgaagccc aagccggcga
     2161 atctgaccag gttatcagcc aaccacaggg gctcattccc cttttggcag gcgtaccgca
     2221 tgatcgatgg acgcgatcac attccgagcc atggcacccg tgatatgccg gtgtttggaa
     2281 tctggttccg tataccggat gatgaagtct cgattgaaac cgagtgggcc gaccaggtac
     2341 gagggcgaat ctggcaactg ttgtcatacc tggaatctat tcaggagcca gaaaaatgac
     2401 tgtgaaggta ctagctatcc atagatgcca ggtaatccac atccctacag atgaacaagc
     2461 aggaggacat gatgtatcaa ccacacaaac acattggttt gctgagtttc atcctcataa
     2521 tggccttgcc cattttggga ttaagccagg aagttccagg caaccctcag aacgggaaag
     2581 ttctgtttga aaaacattgc gcgaattgtc acggaaaaaa agggactggt gatggaccgg
     2641 acaccaaatt tttgttggta cagcccgcaa actttcactc cttggaatca cgatcaaaaa
     2701 ccgattggga attaatgaat gtgatcactt atggagctat attttctcct atgcatgggt
     2761 ggtcagagcg gctgacggaa gatgagcgtt gggatgtttt gcgatatatc agatttcttg
     2821 ctccatttaa ccccatcgcc aacctttacc ctttaccgga gactcacggg taaaaactca
     2881 gcgaatgcag ccatatcggg tgtccaccat tcatttcccc atcaagggga ttcagccatg
     2941 tcggacatgt taaaagtaat cgaggtgtta gccgaatcag acaagagctg agtggattcg
     3001 gcttcgaacg ccgtgggcca ggcagccaaa agtgtgcacg acctaaaatc aatttacatc
     3061 taggatatgg aagccaaggg gaaaatacca agatttccaa atatcgaatt aacgccaaca
     3121 tttctttcct gctggattag aaagaacaga aggacaggtg gattcccgct cctggtcatc
     3181 ctgccccatt cggcaagcct gccctgagcg tagtcggagg ggttcagggc cgggtccacg
     3241 gaagtacctg gctgggccct agcgccgtca acctgggcac agatccttca cgataaactc
     3301 aggatgataa caggggaata acaaatggac ggccttgagt cttcaacacg gtgggaacat
     3361 tttccccatg aagcggatat gggcattcgc gggtttggaa tcacgaaggc ccaagccttt
     3421 gaacaggctg cgttagcgct gacggctgtg gtcacggatc cccaacgggt ggctgctcaa
     3481 atggaacaac acttcacctg cgaagcaccg gatgatgagc tcttactggt cgattggctg
     3541 aatgccctca tctttgaaat ggcatgcggg catatggtat ttgcatggtt tgacgtcacc
     3601 atcaccgatc gtcacctgga gggcatcgca tatggggaac cagtcgatcg catgaaacat
     3661 gaaccagccg tagaaataaa gggggcgacc tataccgagc tgtttgtcag gcggattgag
     3721 ggaaacggtt gggttgccca atgtgtcgtt gatgtctagt cctgggagtt gaatattgca
     3781 ggcatggtaa ccgactaggt cggggtaccg attgatccac tcttccttct gcgaaaaacc
     3841 gtatggacct gaatctccta tcccgagtct ctgcgtatga atggcacatc cctcaaaagg
     3901 gaaagatgcg cgtgccggga attatttacg ccgatgagtc actcgttcgc gacatggatc
     3961 tcaaggtcta tgaacaggtc gtcaatgtgg ccacactccc gggaattgtc caagcctcgt
     4021 ttgccatgcc cgatgcgcat tgggggtacg ggtttccgat aggcggagtc gccgccttcg
     4081 accctcaaca gggaggagtg gtgtctgccg gcggtgtcgg gtttgatatt tcctgcggcg
     4141 tacggaccct ctacaccggt ttgcgtgcgg acgatatcac ccccctgaaa gaaaaactgg
     4201 cgaacctctt atttgcgaag gtgcccgcag gcgtgggtag tacagggcaa ttgcgcctga
     4261 aacccaagga aatggatgac atgctcgctg gcggtgccca atgggcagtc caacgaggct
     4321 atgggacagc aaacgattta tcgcgcattg aagaacatgg ctgcatgacc ggggcaaaac
     4381 ccgggtgtgt gtctgaccat gccaagaaac gccagcagga cgaaatgggt acattaggat
     4441 ccgggaatca ttatttggaa attcaagagg tcgtggaaac ctaccacgat cctttatcgg
     4501 aaaaacttgg tatccaggcc ggggatattc tggtcagcat tcattgcggt tcacgagggc
     4561 ttgggcatca aatcggcacc gaatacctca agaaaatggt ggtgacggca agccaatatg
     4621 gcataacgtt gccggaccgc gaattagcct gtgccccgat cgactctccc ctgggccagg
     4681 agtatttagg ggccatgcgg gcggcgatga attgcgcctt agccaatcgt gaaattatca
     4741 cccatttagt ccgtcaggca tttgtagaaa tctttcctca ggcggcctta cccttgctat
     4801 acgatgtgtc acataacaca tgcaaagtgg aggagcacca gatcggcggc cggagtcgac
     4861 gcgtctttgt ccatcggaaa ggtgccacgc gtgcgttcgg tcccggacat gccgatatcc
     4921 ctcctgacct ccaacctttc ggtcaaccgg tcctcatcgg tggaaccatg ggcacagcct
     4981 cttatcttct ggtaggaacg aaagaaggca tgggtctggc ctttggatcg gcctgtcatg
     5041 gggcaggacg gagtatgagt cgacatcagg ccactcgcca atggaagggc cacagagtca
     5101 ttcaagattt agcagctcag ggaatcctca tcaaaagtcc ttcggcgcga ggagtggccg
     5161 aagaggcacc gggtgcctat aaaaacgtca ccgccgtggt ggatgcggcc gaacaggcgc
     5221 acttggcccg taaggtggcg aaaatgcgcc cgttaatctg tattaagggc taagggctcg
     5281 accatatcaa acgactaaac ccaggacgcc gcaatcgcat cgttcatcaa gacaagggga
     5341 aattgttatg gatcacgctc aacgggaaca gagtcttctc aaggtcttgg aagccttcac
     5401 gaaggggttg acctccttaa aggaggcgat cgccctcgtc cattccgcga acgatcaact
     5461 gccgccaggc gaacaaagaa aaaccattca aaaaaagatt gaaggagcgg aacggatgat
     5521 ccaggttggt catgcggcca tgggacaatc cctgggcttt caactctgtc actgcacgtg
     5581 gccacctcaa gtgatgatca gcagtcacta ttcgaatgaa acgaaggttg aacgattcac
     5641 ctgtcccaat tgcgggaaaa cactttcact tcatggtcga caagcaccgg gggaggctct
     5701 gaccgccttt gacccttatg atgtgtatta aggtgcaatg ccttttgacc ggttttgaaa
     5761 agtaaagaag gagaatcatc ggcatgaagc cagttaccgc aacatctgaa ccctacatcc
     5821 tctggtttga agatattggc ttgcaggata tcagtcgagt aggaggcaaa aatgcctctc
     5881 ttggtgaaat gtaccggaag ctcactcccc atggggtcaa gatccccaac ggtttcgcca
     5941 tcacggctga agcctatcgc tatgtgcttc gtgaagccgg actcgacagc aaaatccaat
     6001 agatcctggg cgacctggat accggcgata aggctaaact ccgtcaacgg gggcgacaca
     6061 tccgacaagc gattattgga gccaccctgc ctccagctct ggtccaggcc atcgaggagg
     6121 cctatgaccg cctgagtgac caatccaccg aaggagccga cgtcgccgta cgaagcagcg
     6181 cgacagcaga ggatttaccc gatgccagtt ttgccgggca acaggaaacc tatctgaatg
     6241 ttcagggcca tcaggcactc cttgaaacct gcaagcggtg ctttgcgtag ttgttcaccg
     6301 accgggacat ttcctatcga gtcgacaaag ggttcgacca tctccaaatc gcgctgtcta
     6361 tcggggtgca acaaatggtg cgttccgatc tggcctc
//
LOCUS       DAORAQ010000147         6392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1808_length_6392_cov_62.851191, whole genome shotgun sequence.
ACCESSION   DAORAQ010000147 DAORAQ010000000
VERSION     DAORAQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1808_length_6392_cov_62.851191"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1151
                     /locus_tag="PKM72_11215"
     CDS             252..1151
                     /locus_tag="PKM72_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61404.1"
                     /translation="MEHVVGARIQQLRTIDPGEIRLVRLALPEEIEIAGKLYAIQDTM
                     RPGTAFLAKPWGDRTGKYRRRMYTRSNASFNEPRVLETFINYTHREQSDTSGWWQSEM
                     ATDWFEKGKTVEVRLQLDEERSVALVYENTKVCTPANLRLEDDGIWEGMRMVCVAFGT
                     GITPFLSYARYLKANNFGRKGARVGAHMTLVASVRHEGQLMLHEELADLEQQFPQHFR
                     YQPVLTRSWPTAWAYLKGRILRVAQSVEGKDQIDIVPFQTIVPDITQSHLRVCGSVTA
                     CRQLTLGLEQEGLIPLTVRTESW"
     gene            complement(1195..1479)
                     /locus_tag="PKM72_11220"
     CDS             complement(1195..1479)
                     /locus_tag="PKM72_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61405.1"
                     /translation="MKKSAQPDQPIGRTAKNSPKKTLTKKASTEKYPRCSRCGSRTVH
                     LEQLEQELLVTLSLHCLICGHYTFLGRPVIRLLRRPNVTIPDSITRPAGE"
     gene            complement(1677..2117)
                     /locus_tag="PKM72_11225"
     CDS             complement(1677..2117)
                     /locus_tag="PKM72_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61406.1"
                     /translation="MLWTFILLMVLLSACTSQPRKPSPPSRSSLPAITGESQTPALEN
                     QLLATVKHAEGLGPGNPLLLSSLYSLATYYEDRKEYDKAAVQYERALKLKEAASGPNH
                     PDVAAILQRYARLLQAANRQPEAANLLLRSEAILAQTSPPSSSQ"
     gene            2304..3395
                     /locus_tag="PKM72_11230"
     CDS             2304..3395
                     /locus_tag="PKM72_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61407.1"
                     /translation="MTVLWCSISAHGFGHAAQLMPILNELDGLVDDLHVILRTKVPAD
                     FFQRHLQEVSWELQDVQQDVGCVQRGPLDIDVARTWEAYTEFHTDWKSRVEKEAQAIR
                     LARASLVISNISPLAIAGAFQAKCPVVGVASLSWDRVLEPYVQADVPGHHVILEIIRN
                     SYALADSLIRLHPGIEMPAFRSIVDVGPSAPVRKPNGYDLRKTLEVGDQELIVLIAFG
                     GVPLNHLPLEQMAAMSGFHFLVSGLPSSISYARVHRVENLEMPFGEISQQVDIIMTKP
                     GYGTVIAAVQDKTALVYVRRKNFIDEQSLVDYTHRYGRGAELSRDDFESGNWEATLRE
                     VLAVEFPSEPPPSPGTGDVVRQLKPYLAC"
     gene            complement(3418..3945)
                     /locus_tag="PKM72_11235"
     CDS             complement(3418..3945)
                     /locus_tag="PKM72_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61408.1"
                     /translation="MTIFSKFPFLGVWVFVLFVGMGYAPIPAISSEAIQEFTTSAPSK
                     PSLFWPLSPPLTSSTFTTSPEQVSIAMLLSNPASYHQRLVAVRGVVTQPELHIDDSEL
                     VMRFVFRLAEGEQSIVIFGEHDRTQGPPSISLDLSVEVIGVFWKDRELNGSHVINTIE
                     AHTVAPYPSLIPDNA"
     gene            4059..4736
                     /locus_tag="PKM72_11240"
     CDS             4059..4736
                     /locus_tag="PKM72_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNP61409.1"
                     /translation="MAVVDYYAVLGIALQASQDEIKKAYRTLALQYHPDRNRGNRQAE
                     QKIREVNAAYEILGDSDARKTYDRMRLGYAEPMAHRRDRDPEPEPEESVSPSVVLERM
                     EGTLREESRKQLFMVLIRNTQKIKEELVIIRERVIRAQGYDTFLEKIVIQRGREVVDE
                     LVTEEFKQRQERLVEIAVEMVCSAVPGSIRGSDQMDQVRRNLAQAYQEGWVQGYEQAC
                     ELLYERR"
     gene            complement(4874..5131)
                     /locus_tag="PKM72_11245"
     CDS             complement(4874..5131)
                     /locus_tag="PKM72_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61410.1"
                     /translation="MTLPEIGAIQFLQPASSGGDHSRKQVTAVNSDEESGKSKSHRER
                     GVASPIQDQVTLSREAQALATSRNPTSKSNSFQQPPSPFDR"
     gene            5599..5790
                     /locus_tag="PKM72_11250"
     CDS             5599..5790
                     /locus_tag="PKM72_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNP61411.1"
                     /translation="MQEFKAHVSRIKDLTHDVRELELNLTAPDSIEFKAGQWISLNVW
                     HPGLNRHVSRQYSIASPPS"
     gene            5848..6330
                     /locus_tag="PKM72_11255"
     CDS             5848..6330
                     /locus_tag="PKM72_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61412.1"
                     /translation="MFGLHEGDPVTFQGPNGSFYLEEKPGRDLVFVATGTGIAPFRSM
                     ISTFLKQPEAGILTLFWGLRSERDLYYQHELATLAERYSNFRFMTTLSRPQNVWKGST
                     GRVTTLVENHIASVSNVTFYLCGNGGMIRDTTAIVRKKGLCPIRTEQYYDKAGAGTGD
                     "
BASE COUNT         1658 a         1406 c         1752 g         1576 t
ORIGIN      
        1 cattgctgtt cccatcagga gatgacgaaa ttgtgacttt aggaggaagt gatggcctct
       61 ctccttgtac ttttggggag atggaagaaa ctggatccat tatattgggt ggtctcaggt
      121 cgagaagaaa ttttaaagaa tataaatgga agaatcaatg acatcgctaa ctctgctcta
      181 ttggcggtga acttctccct atgaaattac tcggcacttt ttgagagttc tcatatagaa
      241 atgagctgat catggaacat gttgtgggcg ctcggattca gcaattacgg accattgatc
      301 caggagaaat tcgcttggtc cggttagcac tgcctgagga gatcgagata gcagggaagc
      361 tatatgccat ccaggatacg atgcgtccag gaacagcgtt tctcgccaag ccctggggag
      421 accggactgg caagtatcga agacgaatgt atacacggtc aaacgcctcc tttaatgagc
      481 cgcgcgtgtt ggagaccttc atcaattata ctcatcgaga gcaatcggat acctcaggct
      541 ggtggcaatc agaaatggca accgactggt ttgagaaggg aaagacggtg gaggtgcgcc
      601 tccaactgga cgaggaaagg agcgtcgcgt tggtttatga aaacaccaag gtttgtacgc
      661 ctgcgaatct tcgtttggaa gatgatggga tatgggaagg gatgcgaatg gtctgtgtgg
      721 ccttcggcac gggaattacg ccatttttat cctatgcccg ttatctaaaa gccaacaatt
      781 tcggtcgaaa gggggcgaga gttggagccc acatgacgct ggtggcgagt gttcgccatg
      841 agggtcagtt aatgcttcat gaggagttgg ccgatcttga acaacaattt ccccaacatt
      901 ttcgatatca accagtgctc actcgatcct ggccgactgc ctgggcctat ctgaaaggac
      961 gaatcctgcg agtagcgcaa tccgtggagg ggaaggatca aatcgatatt gtaccattcc
     1021 aaactattgt gccggatatc acccaatcgc atcttcgcgt atgtggcagc gtcacggcct
     1081 gccgacagct cacactgggc cttgaacagg aagggctaat tccgctaacg gtgcgcacgg
     1141 agtcctggtg acaaaatcgt catgacaagc cggagaattg gtggggatga ttccctattc
     1201 ccctgcgggc cttgtaatgg agtctggaat cgtgacatta ggacggcgta ataaacggat
     1261 cacagggcga ccaaggaagg tgtaatgtcc acagatcagg cagtgcaagc tgagtgtgac
     1321 aagaagttcc tgctccaact gttctaaatg cactgttctc gacccacatc gagaacaacg
     1381 aggatacttt tccgtggagg ccttttttgt cagggtcttt ttgggagagt ttttggctgt
     1441 cctgccgatg ggttggtctg gctgtgctga ctttttcatg aatgaatcct tggtcaagtt
     1501 attagaagaa ctcatttgga tataaccaat tcttgtgcca aacttaaaca gggaaaagta
     1561 gcaggaaagt cccaaaacgg cctagaaaca ggatgaagga agcggacact gggtaaaatt
     1621 ttccgaaaga gcagggtcaa aaatgctgac ctggagagcc gtcgtccggc taaatttcat
     1681 tgactggaag agggaggcga ggtttgagcg aggatggctt cagaacgaag aaggagattc
     1741 gccgcttccg gttgtcggtt agctgcttgc agcaatcgtg catatcgttg aaggatggcg
     1801 gccacgtctg gatggttggg gccactcgcg gcttctttta atttaagagc ccgttcgtat
     1861 tgaacagcag ctttgtcata ttctttccga tcttcataat aggtggccaa gctatacaga
     1921 ctacttaaga gcaggggatt cccgggtcct aagccttcag catgtttaac ggtggccaat
     1981 aattgatttt ccagggccgg ggtctgtgac tctccggtta ttgccggaag cgaggaccta
     2041 gaagggggag agggttttct tggctgggag gtacatgcgc tgagcaggac catcaggagg
     2101 atgaaggtcc acagcattcc aaaagcacat cgtatattcc tgttcatgga acaccccgat
     2161 caacgcattt aaaaaaagac atgaaaagaa aatcagaggt gattctatga actctgaggc
     2221 tgttctcgca agtgaactcc ccgggttgca tttttcagaa taatccctct catgtggtga
     2281 tcctaaaggg aaaagaataa acgatgacgg ttctatggtg ttcgatctcg gcccatggct
     2341 ttgggcatgc ggcgcaatta atgccaatcc tgaatgaatt agatgggttg gtagatgacc
     2401 tgcatgtgat tctacgcacg aaagtaccgg ctgatttctt tcaacggcat cttcaggagg
     2461 tcagctggga gttgcaagat gtccaacaag atgttggttg tgtccagcga gggcccttgg
     2521 acattgatgt ggcgcgcaca tgggaggcct acaccgaatt tcacacagat tggaagtcca
     2581 gggtcgagaa agaagctcag gccatccgat tggccagggc gagtctggtg atatcgaata
     2641 tttcgcctct tgccatcgct ggcgctttcc aggcgaaatg tccggtggtg ggggttgcct
     2701 cgctatcctg ggatcgggtg ttagagccat atgtgcaagc cgatgttccc ggtcatcatg
     2761 tgattctcga aatcatccgt aacagctatg cgttggcaga ctctctcatc cgtcttcatc
     2821 ccggtatcga gatgccggcg tttcgttcga tagttgatgt gggaccttct gctcccgtga
     2881 ggaaaccgaa tggttatgat ctccggaaaa ccttggaggt gggagatcag gaattgattg
     2941 ttcttattgc ctttgggggt gtaccgctca accatcttcc gctagagcag atggccgcaa
     3001 tgtccggatt tcatttttta gtgagcgggc tgccatcttc gatttcttat gcccgtgtgc
     3061 atcgtgtaga aaatcttgag atgccatttg gggagatcag tcaacaggta gatatcatta
     3121 tgaccaagcc gggatatggt accgtgattg ccgcagtcca ggataaaacg gccctcgtct
     3181 acgtccgtcg caagaatttt attgacgaac agagtttagt ggattatacc catcggtacg
     3241 gccggggcgc ggagttatcc cgtgatgact ttgaatccgg caattgggaa gctacgctta
     3301 gagaggtgct tgcggtagag tttccgtctg agcctccccc gtcaccgggc acgggtgatg
     3361 ttgttcgtca gttgaaacca tatttggcgt gctaagggat gcctgtatcg gatctggtca
     3421 agcattgtct ggtatcaggg agggataggg ggcgaccgta tgggcttcaa tcgtattgat
     3481 aacgtgtgat ccattcaatt ctcgatcttt ccaaaaaact ccgatcactt ccacggaaag
     3541 atccaaggaa attgacggtg gaccttgcgt ccgatcgtgt tcaccaaaga tgacaatgga
     3601 ttgttcaccc tcggcaaggc gaaagacaaa gcgcatgact aactcggagt catcaatgtg
     3661 caactccggt tgggtgacta ctcctcgaac ggccacgagt cgttgatgat aggaagccgg
     3721 gttactcagc agcatggcaa tggagacctg ttcgggtgat gtggtaaaag tggaggaggt
     3781 gagtggagga gaaaggggcc agaagagact gggtttcgat ggcgcagaag ttgtgaactc
     3841 ctggatagct tccgaagaaa ttgccggtat aggcgcataa cccataccaa cgaacaggac
     3901 aaaaacccat actccaagga acgggaattt tgaaaaaatg gtcatgaaag aaaaattgtt
     3961 ttttaaaaac ccgatgcagg ggccagtgaa agtgaatgtt cccaagaaac tgtgcaaaag
     4021 cttgggaaaa gaatttcctc ctgtaaggaa tcatgtgaat ggcagtcgtt gattattatg
     4081 cggttcttgg aatcgcgctt caagcctcgc aggatgagat taaaaaagcg tatcgcactt
     4141 tagcccttca atatcatcct gatcgcaatc gagggaatcg tcaagctgaa caaaaaattc
     4201 gtgaagtcaa tgcggcctac gaaatcttgg gagattcgga tgcccgaaaa acgtacgatc
     4261 gaatgcgact gggctatgcg gaaccgatgg cgcatcggag ggatagagat cctgaacctg
     4321 agccggaaga atccgtttct ccttcagtgg tcctggaacg aatggaagga actctccggg
     4381 aagaatcccg caagcaactc tttatggtgt tgatacgcaa tacacaaaaa atcaaagaag
     4441 aattagtcat tattcgggaa cgggtcattc gtgcccaggg gtacgatacc tttctggaga
     4501 aaattgtcat tcagcgaggc cgggaagtgg tagatgagct ggtaaccgag gagttcaagc
     4561 aacgtcaaga gcgccttgtg gagatagccg tggagatggt gtgctcggcg gttccaggtt
     4621 cgattcgagg ctcagatcaa atggatcagg ttcgacgaaa tttagcgcag gcctatcagg
     4681 aaggatgggt tcaagggtac gaacaagctt gtgaactgct atacgagcgc aggtaatgag
     4741 gccggattta ttgagaaaat tgtcaaggca ttaaggaaag gggaatgatg gactcatgag
     4801 gcaagccttg ttcaaaaatc ggggccagca aacgggatgc cccatttgcc gggcgatcgc
     4861 agtttcagga atgtcagcga tcaaatggag atgggggctg ctgaaatgaa ttgctttttg
     4921 aggtcgggtt ccttgatgtg gccaatgcct gagcctccct cgacaaggtc acctggtcct
     4981 gaatgggtga ggcgacgcct cgttcccgat ggctttttga tttgccactc tcctcatcag
     5041 aattaacggc agtcacctgc tttcgtgaat gatcccctcc tgatgaggcg ggttgcagaa
     5101 attgaatagc gcctatttcc ggtaatgtca tggtcaggac tccttctctc tgccgaatta
     5161 tttgacaaac ttccctttca ttaccctttg tcggtaggga agccaaatgc ttgcgacctg
     5221 ggcttgggtg aggtaagcaa aaagacacga aatagaggac tatcttcaga aaacctgcat
     5281 gaatcggata aaacgcaccc aaggaagaat cagcatcaaa tatcccaaac cggataaaag
     5341 gcatgtgtgg caaaaagaaa ccggtgagca gtgaaactcc gcccagctgg ttaggggaag
     5401 tttcctctct ttctcccctt attatatttc cagatgcatg agtgctcttt gccttgcgaa
     5461 atcagccaat gacgggcaat tgtcatcatt ggtgtttagc gcggcatttt tccataaaat
     5521 tttggatggg gattctggtt tggtaatgtg taatgtagct ctctagtgag tgagctcaaa
     5581 ctgcagggtc ccgaaataat gcaagagttt aaagcccatg tcagtcgaat caaggacctg
     5641 acccatgatg tccgggagtt ggaactcaac cttaccgctc cggattcaat tgaattcaag
     5701 gccgggcagt ggatctctct gaacgtctgg catccgggat tgaaccgaca tgtttcgcgg
     5761 cagtattcaa ttgcctcccc accgagttag taccaacaga tcactctcct ctttaaccgg
     5821 gttcccgatg gcccaggctc cagctatttg tttggccttc atgaagggga tcccgtaaca
     5881 tttcaagggc ccaatgggtc gttttatctc gaagagaaac ccggacgaga cctcgttttt
     5941 gtggccaccg gcaccggtat cgcgccgttt cgttcgatga tctccacatt cctaaaacag
     6001 ccggaagctg gaattctgac gttattttgg gggttgcgga gcgagcgaga cctttactac
     6061 caacatgaat tggctaccct tgcagagcgg tattccaatt tcagatttat gaccactctc
     6121 tcccgtcccc aaaatgtttg gaaaggatcg accggaagag tgacaacatt agtggagaac
     6181 cacattgcat cagtgagcaa tgtgacgttt tatctatgcg gaaatggcgg catgatacga
     6241 gacacgaccg ccattgtgag aaaaaaaggc ctctgtccca ttcgcactga acaatactat
     6301 gacaaagccg gggccggaac aggcgattga atctggcccg actctacgga taaaacaggt
     6361 tgatgccaat gattgttgag ccaaacaaca ag
//
LOCUS       DAORAQ010000148         6376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1814_length_6376_cov_77.810157, whole genome shotgun sequence.
ACCESSION   DAORAQ010000148 DAORAQ010000000
VERSION     DAORAQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_6376_cov_77.810157"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..173)
                     /locus_tag="PKM72_11260"
     tRNA            complement(98..173)
                     /locus_tag="PKM72_11260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(138..140),aa:Thr,seq:ggt)
     gene            complement(243..317)
                     /locus_tag="PKM72_11265"
     tRNA            complement(243..317)
                     /locus_tag="PKM72_11265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(283..285),aa:Gly,seq:tcc)
     gene            complement(408..492)
                     /locus_tag="PKM72_11270"
     tRNA            complement(408..492)
                     /locus_tag="PKM72_11270"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Tyr,seq:gta)
     gene            810..1355
                     /locus_tag="PKM72_11275"
     CDS             810..1355
                     /locus_tag="PKM72_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNP61413.1"
                     /translation="MKRPHLPSQQRKRLKFLTWIVVIPSFLCMGLIIGSYLNAFGEIN
                     DRQAKGLARIATPNGEIIFAEVADTLDKRAQGLMYRTAMDENHGMLFIFPELGYWTFW
                     MKNTKIPLDILWLDQKGTIIHIEANVPICTRVDDHCPRHYSYKQSWQVLELNAGTAEK
                     FQLQPGSRLTISLPSQNLNPS"
     gene            complement(1373..3547)
                     /locus_tag="PKM72_11280"
     CDS             complement(1373..3547)
                     /locus_tag="PKM72_11280"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNP61414.1"
                     /translation="MGATHLTDIEQLMDQVKAYYPEADTNFIRRAYDYSAKAHDGQTR
                     QTGEPYVQHPLAVAGILTFLKLDVPAIVAGLLHDTLEDTVATRAELEKEFGSDVARLV
                     EGVTKIGQIPFKTDEEKQAENFRKMVLSMADDIRVVFIKLADRLHNMQTLEALSDEKR
                     KKIAQETMEIYAPLANRLGMSWIKQQLEDLCFQYLEPDAWSHLKLRLAKKDEERQEYI
                     QSLIHLAQQALLSVGLPGQIDGRPKHLFSIYQKMERRSVSFDEIFDLTAVRIVTDTKM
                     NCYAILGLLHSIWPPVPGRFKDYVATPRSNLYQSLHTTVVGPQGEYVEFQIRTADMHR
                     INEYGVAAHWLYKEPGKVGGRDEQVFSWLRQFVEWHKDLADNRQFMDSVKLDLFHDVV
                     FVFTPTGEVREIPVGATPLDFAYAIHTEIGDHCVGAKINGKLVPLRYQLSSGDMVEIL
                     TSPSQVPHRGWLKFVRTSRAKTKIKHWFKVEEQKRALELGRRLLEQEFRRHNLPVAQS
                     LKSERLSSMAKAKGSETVDELIRQVGFGRLSPDQIVSHFYEGAEQGPSPSTKGVPLFP
                     QQKMPGLLREGSVKFGGTKDVLMQLSKCCKPVPGEPIRGYITRGRGLTIHAIDCPNFQ
                     SLEWDHNRLVEVEWDSELDGTHSVEVSVLTLDRTGVLAKVSAGISQCQANITRAEIST
                     REDRKAVLDFVIEVKNTAHLELILQEIQRVDGILSAKRVRGW"
     gene            complement(3880..4083)
                     /locus_tag="PKM72_11285"
     CDS             complement(3880..4083)
                     /locus_tag="PKM72_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HNP61415.1"
                     /translation="MDKQERKNESRKPSSPKESEDVRADPSVAETGQRLKEEMDELVD
                     EIDKVLEENAAEFVKNYVQKGGE"
     gene            4221..4295
                     /locus_tag="PKM72_11290"
     tRNA            4221..4295
                     /locus_tag="PKM72_11290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4253..4255,aa:Val,seq:cac)
     gene            4475..5656
                     /locus_tag="PKM72_11295"
     CDS             4475..5656
                     /locus_tag="PKM72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61416.1"
                     /translation="MVKSINHHNRLRKKPAISFKAIALFCLLPHCASTPPPSNLPPSE
                     SDLNLLGKATLCQTKAEAQRTWLQPGMQGSPWGGGKEIFRETASPNKQAQWFMFNEDQ
                     TLVGVITVFPLGLSLEDYPKLRHTLAQLPPAREFFFHSSQLLKEQTPDSGTLHRTGEA
                     TTTHQYFMRHTNGKQDQLVMAVFVLDPYETLLDGSHSKFLSYIKDPDSSEKEPPNPSG
                     QSESEKEFLGLQQFARGEIALFASCGNKQPEIALDAYQKAIQYGLSDPKQLAEAHHRL
                     GLALRSMGRLTEATTTLEQALTIQPYSAVILNSYGSVLTELGKLPKAIETYEKALALQ
                     PNYAQARFNLAEAYESHNPKRAIQEYETFLILAGNNPEEATKVDLAKNRIKALQGGVR
                     Q"
     gene            5750..6373
                     /locus_tag="PKM72_11300"
     CDS             5750..6373
                     /locus_tag="PKM72_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNP61417.1"
                     /translation="MKFYAVRRGRTKGIFESWDACRAQVHHFPGAEYKAFPTRAEAEA
                     FLSFRLSSEPIKNKPVAAETTSPSTIEVWVDGSCFPQKDGSLRLGWGLLAKKNGVEIY
                     RDKGNDIPRSAMNHRNVAGEILAILKAVKWCQSQGITEMTIYFDYQGLESWVTGAWRT
                     KLPFTEAYARTVIESGISIHWVKVKAHSGNPENDLVDQLAKEGAQGK"
BASE COUNT         1781 a         1667 c         1323 g         1605 t
ORIGIN      
        1 cacattgaca tgcggcttct tccgctcaaa cttcgccttc gccatgccct acctcttcct
       61 taatgctata cgcaataatt taaaatttta tcgttgctgg agcccacgac gcggatcgaa
      121 cgcgtgacct cgtccttacc aaggacgtgc tctaccaact gagctacatg ggctctacac
      181 cactcactcg aaaagatctt cgcctcaaaa aagcacacaa atctaagccc acccagaaac
      241 aatggagcgg gcgacgggat ttgaacccgc gacagccagc ttggaaggct ggaactctac
      301 cactgagcta cgcccgcagc ttggtttaaa ccctatatac cgagaaactt ttgcctcaac
      361 agcacatttt gtcgtatcca ctagatcccg tccttttcag tcagtcatgg tgggcaggca
      421 agggttcgaa ccttggaagc cgatggcagc agatttacag tctgcccccg ttgaccgctt
      481 gggtacctgc cctttgaatg agtaggaccg tcttctactt aaaccgcttt ggaattacat
      541 catggaaatt attaagatga agatcttttt gaagtgaaga caaacaaggc agaaattagc
      601 ccctgcatgt acagatcaac aacaaaaaca ttcgcgtttt gttctaaaaa caggagcgga
      661 acaaattcac tcttcttgta aacgaatcat tataatttcc aaatatggtc tggtcaagac
      721 ccttgtcctg cattttttca gacaggaaaa tgccttattt gacaaatcct ttctccccaa
      781 gccacattca tgaaataatg gatttcctca tgaaacgccc tcacctacca agccagcaga
      841 gaaagcggct gaagtttcta acttggatcg tcgtcattcc ttctttctta tgcatggggc
      901 taatcattgg atcttactta aacgcttttg gggaaataaa tgatcgacag gcaaagggcc
      961 ttgctcgcat cgcgacaccc aatggagaaa ttatttttgc cgaggtggca gacacgctgg
     1021 ataaaagagc tcaaggactg atgtatcgga ccgccatgga tgaaaatcat ggcatgttat
     1081 ttattttccc agaattgggg tattggacct tttggatgaa gaacacaaaa atcccgctgg
     1141 atattttatg gctggaccaa aagggaacca ttattcacat cgaagcaaac gtcccaattt
     1201 gcacgagggt agatgaccac tgcccacgcc actattcgta caaacagtcc tggcaggtcc
     1261 ttgaactcaa tgccggaaca gcagaaaagt tccaactcca accaggaagc cgactcacaa
     1321 tttccctgcc ttcccagaac ctcaatccct cctaactgcg cctaccattc acttaccacc
     1381 ctctcacccg cttggcagaa agaatgccgt caacccgttg gatctcctgt agtataagtt
     1441 caaggtgtgc agtattttta acttcgatga caaaatctaa cacggctttt ctatcctccc
     1501 tggtagaaat ctctgccctg gtaatgttgg cttgacactg agaaattcca gcggaaacct
     1561 tagccaagac tcccgttcga tccagggtta aaactgacac ttcgactgaa tgggtgccat
     1621 caagctccga atcccactca acctccacca atcgattgtg atcccattct aatgactgaa
     1681 agttcggaca atcgatcgca tgaatagtca atcccctccc acgagtaatg tatccacgaa
     1741 tcggctcacc gggaacaggc ttacaacact tggaaagttg catcaagacg tctttggttc
     1801 ccccaaactt tacagatccc tcccgaagga gacctggcat tttttgctga gggaagagag
     1861 ggacaccttt cgtggaaggc gaaggccctt gttcagcccc ttcgtaaaaa tgactgacaa
     1921 tttgatccgg ggacaaccgg ccaaatccaa cctgccgaat taattcgtcc actgtttcac
     1981 tgccctttgc cttagccatt gacgagaggc gttccgattt taaggattgc gcgacgggaa
     2041 gattgtgtcg ccgaaattct tgttccaaca aacgtcgacc aagttccagt gcccgctttt
     2101 gctcttcaac cttaaaccaa tgcttgattt tggttttagc ccgtgaagtt cggacaaatt
     2161 tcagccaccc gcgatgaggc acctgcgacg gggaagttag aatttctacc atatccccac
     2221 tgcttaactg ataacgaagg ggaactaatt tcccattaat ttttgccccg acacaatgat
     2281 ctccgatttc cgtatgaatc gcatacgcaa agtctaaagg ggtggccccg acaggaatct
     2341 cccgcacttc ccctgttggt gtaaacacaa acaccacgtc atggaataaa tccaacttaa
     2401 ccgaatccat aaactgacgg ttatcggcaa ggtccttatg ccattccaca aactgtctaa
     2461 gccaactgaa cacctgttcg tcccgtccgc caactttccc tggctcctta tacagccaat
     2521 gggctgctac accgtattca ttaattcgat gcatatccgc cgtccgaatt tggaattcca
     2581 catactcacc ttgtgggcct accaccgtcg tatgcaacga ttgatacaag tttgaacgtg
     2641 gagtcgcgac gtagtcttta aagcgaccag ggacgggagg ccaaatggaa tgcagcaagc
     2701 ccaatatggc gtaacaattc attttggtat ccgtcacaat gcgaaccgcc gtcaaatcaa
     2761 aaatttcatc aaaagatacc gaccggcgct ccattttctg ataaatgctg aagagatgct
     2821 tgggtcgccc atcaatctgt cccggcagtc cgacgctcaa gagtgcctgc tgagccaggt
     2881 gaatcaggga ttgaatatat tcctgtcgct cctcatcttt tttggccaaa cgtaacttca
     2941 aatgagacca ggcatccggt tccaaatact ggaaacacag atcctctagc tgctgtttaa
     3001 tccaactcat accaagtcga ttggctaaag gcgcataaat ttccatggtt tcttgcgcaa
     3061 ttttttttcg tttctcgtcc gataaggctt caagggtctg catattatgc agtcggtccg
     3121 ctaatttgat aaacaccact cgaatatcat cggccataga gagaaccatt ttacggaaat
     3181 tttccgcttg cttctcttca tcagttttaa aaggaatttg cccgattttt gtcacaccct
     3241 cgactaaacg agccacgtca cttccaaatt ctttttctaa ctcggctctg gtcgctacgg
     3301 tatcttcgag agtgtcatgt aatagcccag caacaatcgc gggaacgtcc aattttagaa
     3361 aagttaaaat cccagcgact gctaacggat gctgaacata gggttcaccg gtttgtcgag
     3421 tctgaccatc atgcgctttc gcagaatagt cataagctcg tctgataaag ttggtatcag
     3481 cctcaggata ataggctttt acctgatcca taagctgttc aatatccgtc aagtgcgtcg
     3541 cgcccatttt ttttcagtcc ttcatcaaag tgtctcacgg cattgtcaat gaatcataac
     3601 aaaaaagaga gaaaaatgct gttttttcat tccagatgcg gcgatccgcc ctcggcggaa
     3661 taaaagaaag aggtgcaatt gccctccctt aagagataca tcatccaaga gcggattcat
     3721 cgcaccgaac cagaaataat aatatgcgag tagttgagag gcctaagagc ctcactcaga
     3781 aatgacgata aaagaaacag ggaacagcaa tggctctaga atgtctggcg agtagaagag
     3841 ggcaggtaat gattttagaa gtcaacgaat cttacgcatc tattccccac ctttttgtac
     3901 atagtttttg acaaattcag ctgcattctc ttctaacact ttatcaattt catcaaccag
     3961 ctcatccatt tcttctttta agcgctggcc ggtttccgca acactcggat ctgctcgaac
     4021 atcctctgat tcttttggag agctcggttt tcgagattca tttttccgct cttgcttgtc
     4081 catggtgcac ctccttggtc aagtctatga aacccctctc ttcagcctac tccacacttt
     4141 ctccaaccgt caagaaaagc aatgccgccc cctcggaatt cgttgctccc ttcctagcat
     4201 tgtgataaat tgcctcccgc gggcgattag ctcagtggaa gagcgcttcc ttcacacgga
     4261 agaggccact ggttcgatcc cagtatcgcc caccatggta ttgcaacttt tttgaatcaa
     4321 ttccttttcg gcatagttgt gagtcgcgcc atcgcaagta ggatggagac cttcttgatt
     4381 ctgcatttgt cactcacaat taaattcgga ctctagcacc caagtcaccc ttcccttcaa
     4441 ccaatcgacc atccaccccc aagagacggt gataatggta aaatcgatca accatcacaa
     4501 tcgactccgc aaaaagccgg caatttcctt caaagcgatt gcgctttttt gcttactccc
     4561 acattgcgcc agcaccccac ctccctcgaa ccttccgcct tcggaaagcg acctcaatct
     4621 cttaggaaag gccactttgt gccaaaccaa agcagaggct caaaggactt ggctgcaacc
     4681 agggatgcag ggatctccat ggggcggagg caaggaaatc tttcgtgaaa ctgcatcacc
     4741 caacaaacaa gcacaatggt tcatgttcaa tgaagaccag acccttgtgg gggtcatcac
     4801 cgtattcccg cttggccttt ccttagaaga ttatccgaaa ctccgtcata ctctggcgca
     4861 attacccccg gctcgtgaat tcttttttca ttcttcccaa ctcttaaaag agcaaacacc
     4921 cgattctggc actctacacc ggacgggaga agccacgacc acccatcaat atttcatgcg
     4981 acacaccaat gggaaacagg accaactggt catggcagtt ttcgttcttg atccctatga
     5041 aacgctttta gatggcagtc attcaaaatt tctctcctat attaaagatc cggattcctc
     5101 ggagaaagaa cccccaaacc caagtggaca atcagaatcg gaaaaggaat ttttaggact
     5161 tcagcaattt gccagagggg agatcgccct atttgcgtcg tgcggcaaca agcagccaga
     5221 aatagccttg gacgcttatc aaaaagctat tcaatatgga ttatctgatc cgaaacagtt
     5281 ggcggaagcc caccatcgtc ttgggttggc cttacgaagc atgggtagac ttacagaagc
     5341 cacaactaca ttagagcagg ccctgaccat ccagccatac tcagcagtca tcctaaatag
     5401 ttacggctca gtcctgacgg aacttggaaa attacccaag gctatcgaaa cctatgaaaa
     5461 ggctttggcc ttgcagccaa actatgctca ggcccgattc aatttggctg aggcttacga
     5521 atcccataac cccaaacgcg ccattcaaga atatgaaact ttcttgatac tggcggggaa
     5581 caatccagag gaagcgacaa aagttgactt ggctaaaaat agaataaaag cactacaagg
     5641 cggagtcaga cagtagaaaa ttaagaaaca atcgttatga gaaatacccg gtcaacagta
     5701 ttcgtagcaa aaagatctac gttgtgaatg ccccacacgc taagccttca tgaaattcta
     5761 tgccgttcgc cgcggacgca caaaaggaat ttttgagagt tgggatgcct gccgtgccca
     5821 agtacatcat tttccaggag ccgaatataa agctttcccc acccgagcag aagctgaagc
     5881 atttctttct tttcgtttaa gcagtgaacc aatcaagaac aagccagttg cagcagaaac
     5941 aacatcgcct tcgacgatag aagtttgggt agatggatcc tgcttcccac aaaaagatgg
     6001 ctctcttcga ctgggatggg ggttactggc caaaaaaaat ggtgtagaaa tttatcggga
     6061 caaggggaac gacattccgc gatctgccat gaaccaccga aatgtcgctg gtgaaattct
     6121 tgccatcctc aaagctgtca aatggtgtca atcccaaggg attacagaaa tgacgatcta
     6181 ctttgattac caaggattgg aaagttgggt aaccggagcc tggcgcacca aactgccgtt
     6241 taccgaagcc tacgctcgga cggtcatcga atcaggcatc tctattcatt gggtcaaagt
     6301 gaaggctcac tccgggaatc cagaaaatga tcttgtcgat caacttgcga aagaaggggc
     6361 tcaggggaag taagaa
//
LOCUS       DAORAQ010000149         6371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1816_length_6371_cov_67.657536, whole genome shotgun sequence.
ACCESSION   DAORAQ010000149 DAORAQ010000000
VERSION     DAORAQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_6371_cov_67.657536"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..556)
                     /locus_tag="PKM72_11305"
     CDS             complement(239..556)
                     /locus_tag="PKM72_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61418.1"
                     /translation="MTSLPLSFRVKNAVVEKHQLEGMDPSDRYFNRMIPIKRVERGYS
                     GTVMYEALNLQSQVYRTVQETLKDITDQLRELGFSAMRTRLNFKGQAYLAEKETWVDY
                     IDA"
     gene            728..1300
                     /locus_tag="PKM72_11310"
     CDS             728..1300
                     /locus_tag="PKM72_11310"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNP61419.1"
                     /translation="MLRESSGADIPINLPNSLTVLRILLVPVFVGFLLYEYYDYALVT
                     LLVAALTDGLDGAIARITDQRTRLGEYLDPLADKLLLMSAIVTLSVLHFIPVWAVILV
                     VSRDAILLTGTILANLTEIDIDITPTWLGKGTTFAQICYVIMVILFATGRVPSESIIP
                     FLSVMVILTTGSGVHYLFRGIQRLNSSGKK"
     gene            1321..3138
                     /locus_tag="PKM72_11315"
     CDS             1321..3138
                     /locus_tag="PKM72_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNP61420.1"
                     /translation="MNRERVGLQLVDAKVISSDDLSQALDIQQGEGGRLGSILVRMGV
                     LSESTLLEFLSQHYGVATVELSTCSIDGSLRGLVPYDVVCRHLVLPVRKTTSRLSLAM
                     ADPTNASLLDDLRFRTGLHIIPMVATESDLRTAISHMYSQAPDGSFSTTEPLQKDEQG
                     NLTNRGDSDRMSPNSMGKPAGPVCASPNGLNGEERSSLLQGNAEMSRVKRHIKIDKDS
                     SAVEVVNGLVQQAIGMEASDIHIEPMETMIRVRFRLDGVLCPIQNLPKDLHQALLARV
                     KILSDLDIAERRLPQDGRMKVEGFPHVDIRVAILPCLFGEKAVLRLLNQSGLALHLTN
                     IGLNQPDLDRVTTALENPYGMILVTGPTGSGKTTTLYSALQFLNTPQMNIVTVEDPVE
                     YQIQGINQMQIHEEIGLNFAAGLRAFLRQDPDVMMVGEIRDRETAQIAIQASLTGHRV
                     LSTLHTMNAPGAIARLIDMSIEPFLVSSAVSLIVGQRLVRKICDHCREPDTVSQFQLR
                     ELGIDENEFGAVQAMKGRGCASCHLTGFKGRMALFETLPIFEGLHEKILARVSTNDLI
                     TCAISEGFRTLRQAGMAAVQGGLTTVGEVFSETRSDAFV"
     gene            3163..4275
                     /locus_tag="PKM72_11320"
     CDS             3163..4275
                     /locus_tag="PKM72_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNP61421.1"
                     /translation="MELTVLLDELCRQGGSDLHLVSGSVPRLRVDGHLRPMEFPVLVS
                     QDISRLTASLLTEAQHHQVMEAGEWDGAYSVPAIGRFRVHVYTQRGSLALAIRAVSVE
                     IPTFVALGLPPIVEELMRKPQGLILVTGPTGSGKSTTLASMLDHINEERRAHIISLED
                     PIEILHAHKKSLVSQMEVGSDVRDFQSALKGILRQDPDVVFLGELRDLETIQAALTMA
                     ETGHLTVATLHTNSAIHTLTRLVSVFPPHQQQEIRIQLSMVLEGILSQRLLSRSEGKG
                     RVLALEILISSPAIRNLIREDKIHQMYSMMQTGQAQYGMQTMNQALADLAGQGVISSE
                     LAMGLTTLPDELSKLLERTGRDRSGGMSLARLRNRM"
     gene            4330..5562
                     /locus_tag="PKM72_11325"
     CDS             4330..5562
                     /locus_tag="PKM72_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNP61422.1"
                     /translation="MPRFEYRAKNLKGQAVHGEVLAGTSSEALQLLRRQAVLVTSLQE
                     KVEKVFNLSCLVTGWNRQGTWRAVRSKELVVFTHQLATLIRAGVPLLECLDILSTEAE
                     STTLQQVVKHIREDVEGGTLLAHALKRHPTVFHEFYRSMVEVGETTGRLDESLTQLAV
                     YLGKQAQLRAKIFSGLAYPALLVTVAMIVLVFLLIWVVPLFSGLFQDMGESLPWLTQV
                     VIDVAEGVRDYFFLLTIFVGSLVMGIRWMLKNPKSRQAFDGWVLRVPLLGSVIQKAAT
                     VRFSRTLGFLVRRGVPLLSALGVAGTVTGNKVFEQSIKLATTAVQDGMPISEALRARQ
                     VFPPMVPQMLKVGESTGSIDVMLEKIADFFEQEVDRTVATLTSVLEPFIILVVGCGIA
                     LVVVAMYLPIFSIGSVIG"
     gene            5596..>6371
                     /gene="ispE"
                     /locus_tag="PKM72_11330"
     CDS             5596..>6371
                     /gene="ispE"
                     /locus_tag="PKM72_11330"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNP61423.1"
                     /translation="MSVTEIVVRAPAKVNLLIRVLDQLPNGYHNLWSLMHTVDVFDLL
                     RIRLHSDREGLALTCGDAPLPLDKGNLVYRAAELVLQRAEKTVGVDIELTKVIPLSAG
                     LGGGSSDAAATLYGLAHLLGLNWTLSDLCEAGATLGSDIPFFFRAPCAVVRGWGQDVV
                     ACSIQGERWVVLVNPGFPIQTKWAYKQLASQRDAVRPLDDSTKKIDRELSLSWEEVIG
                     TMENDFEAPLFPFYPILGFIKDTLLSFGAQAALLSGSGATV"
BASE COUNT         1432 a         1403 c         1763 g         1773 t
ORIGIN      
        1 ttttttatga aaagagatag atagttggtg agagcatgac cagagaacat caggcacgga
       61 gtcgagaagt gccactcaga cacatgtgcc caaaatttta caatgtttta tacggttaag
      121 ctgttcaggg agtgagtgtg gtaatcaaag gaaggaccag gcggctgtgt gtcattaaaa
      181 aaaactaggc ggaatagaag ggacatcatt gatgatggct tagatgcgag tacccagatt
      241 aggcgtcgat gtaatcaacc caggtttctt tttctgccag gtatgcctgg cctttgaaat
      301 tgaggcgggt gcgcatggcg gaaaatccca attcacgaag ttgatctgta atgtccttca
      361 gtgtttcctg cacggttcga tagacttgtg actggagatt cagcgcttcg tacatgactg
      421 ttcccgagta tccgcgttcc acccgtttaa ttgggatcat tcggttgaaa tatcggtcac
      481 tagggtccat gccctccaat tgatgctttt cgacgacggc atttttgact cgaaaggata
      541 agggaagcga tgtcatgtgc tctagctccg gcttaacgat agggtatagg tgttttttct
      601 ttctatagaa acggattata ggctgcctcg atgggggtcg caagcgtggt tgacttccct
      661 cagaatccca acgtaccctt ccctacgttt gggaaaaatt cgtttgcgag aaaggggttg
      721 tggcgaaatg ctccgcgagt catcgggtgc cgacattcca atcaaccttc ccaacagtct
      781 caccgtcctt cggattctcc ttgttcctgt ctttgttggg tttttgctct acgaatacta
      841 tgattatgcc ctggtgacat tgttagtcgc ggcattgacc gatgggctgg atggggcaat
      901 agcccgtatc accgatcagc gcactcgcct cggcgagtat ctcgatcctc tggctgacaa
      961 attactcctc atgtcggcca ttgtgaccct atctgtgctt catttcattc cggtctgggc
     1021 agtaattctt gtggtgagtc gcgacgccat tctcctcacg gggaccatat tggctaattt
     1081 aaccgaaatc gacatcgata ttactcccac ctggttggga aagggcacca cctttgcaca
     1141 aatttgctat gtcatcatgg tgattctctt tgcgaccggg cgtgttcctt ccgaaagcat
     1201 cattcctttt ctttccgtaa tggtcatcct gacaaccggt tctggagtgc attatctatt
     1261 tcgcggtatt caacggctca attcttcagg caagaaataa gagtacacgg gagaatacag
     1321 gtgaatcgag agcgtgtcgg cctacagctg gtggatgcga aagtgatctc ttccgatgat
     1381 ctgtctcagg cgcttgatat ccagcaggga gaaggagggc gcctcgggag cattctggtt
     1441 cggatggggg ttctgtctga atccacttta ttagagtttc tcagccagca ctatggagtt
     1501 gccaccgtag aattgtctac gtgctcgatt gatggaagtc ttcgcgggtt ggttccttat
     1561 gacgttgtct gccggcacct cgttttaccg gtgcggaaaa ccacttcacg tctgagtctg
     1621 gctatggccg accctacgaa tgcctctttg ctcgatgatt tgcgatttcg gacagggctg
     1681 cacatcattc ccatggtagc taccgagtcg gatttacgga cagcgatctc ccatatgtat
     1741 tcccaggctc cagatggatc tttctcaacc acggaacctc tgcaaaaaga tgaacagggt
     1801 aatttgacaa accgcggaga tagcgaccgg atgtcaccca attcgatggg gaagcctgcc
     1861 ggccctgtct gtgcctctcc gaacgggttg aatggtgaag aaagatcgag ccttcttcag
     1921 ggcaatgcag agatgtctcg tgtgaagcgt catatcaaaa ttgataaaga ttcttcagct
     1981 gtggaggttg taaatggtct tgttcagcag gccattggaa tggaggcgag tgatattcat
     2041 attgagccga tggagaccat gatccgggtt cgttttcgat tggatggcgt cttgtgcccg
     2101 attcaaaatc tgcctaaaga tcttcatcag gcattacttg ccagagtgaa aattctttcc
     2161 gatctggata ttgcggagcg tcggttaccc caagatggac ggatgaaggt ggagggcttt
     2221 ccccacgtcg atattcgagt agcaattctt ccgtgtttgt ttggggaaaa agcggttctg
     2281 cgtcttctga atcagtcagg gcttgcattg catttgacca atatcgggct caatcagcct
     2341 gatttggatc gggtcacgac ggccctggaa aatccctatg ggatgatcct ggtgacaggg
     2401 cctacgggga gcggaaagac gacaaccctt tatagcgcgt tacaatttct gaacactccc
     2461 cagatgaata ttgtcactgt cgaagatcct gtggaatacc aaatccaggg catcaatcag
     2521 atgcaaattc atgaagagat tggattgaat tttgccgccg gattgcgtgc ttttctgcgg
     2581 caggatcctg acgtcatgat ggtgggggaa attcgtgatc gggaaaccgc gcaaatcgcg
     2641 attcaggctt ctctgactgg ccatcgagtt ctttcgaccc tgcataccat gaatgcccca
     2701 ggggcgattg cccgcctcat cgatatgagc attgagccgt ttttggtgtc ttccgccgtt
     2761 tcgctcattg tgggtcaacg attagtacgg aaaatttgtg atcattgtcg ggagccggat
     2821 acggtatccc aatttcagct gcgggaactg gggattgatg agaatgagtt tggtgcggtt
     2881 caagcaatga aagggcgtgg gtgtgcgtcc tgccacctga ctggatttaa aggacgtatg
     2941 gccttatttg agaccttgcc tatttttgaa gggctccatg aaaaaatttt ggcccgagta
     3001 tcgacgaatg acttgataac ctgtgcgata agcgaaggat ttcgcacact tagacaggcg
     3061 gggatggcgg ctgttcaagg tggcttgacc actgtcggcg aagtattttc tgaaacgagg
     3121 tctgatgctt ttgtgtaagt accccagtag accggtcgac taatggaatt gactgtactt
     3181 ctagatgaat tatgccggca gggtggatcc gatttgcacc tggtctccgg tagtgtgccc
     3241 cgacttcgag tggacgggca tttacgtcct atggagtttc cagtattggt tagccaggac
     3301 atttcccggt taacagcgag tctgttaacg gaagcacaac atcatcaggt tatggaggcc
     3361 ggagagtggg atggagcata cagcgttccc gctattggtc ggtttcgggt ccatgtctat
     3421 acccagcggg gatccttggc cctggccatt cgggcagtat ccgttgagat tccgacgttt
     3481 gtagcattag gccttccgcc tattgttgag gagctgatga ggaagcccca agggctgatt
     3541 ttggtaaccg ggccgacggg aagcggaaaa agtacgacat tggcctcaat gctagatcat
     3601 attaatgagg agcggcgtgc tcatattatt tcgttggaag atcccattga gattctccat
     3661 gcacacaaaa agagcctggt gtctcaaatg gaagtcggat ccgatgtccg cgatttccaa
     3721 tccgccttga aaggcatctt acggcaggat cccgatgtgg tgtttttggg agaattgcga
     3781 gatctagaga ccattcaggc ggcccttaca atggccgaaa ccggtcatct gacggtggcc
     3841 acattgcaca cgaattctgc aattcacaca ctgacacgat tggtctcggt gtttccccca
     3901 caccagcaac aagaaattcg cattcagtta tcgatggttc tggaaggtat tctgtctcag
     3961 aggctcctgt ctcgctccga aggaaagggc cgagtcctgg ctttggaaat tctgatttcc
     4021 tccccggcca ttcggaacct gattcgggaa gacaagattc atcaaatgta ttccatgatg
     4081 caaacggggc aagcccaata tggcatgcag accatgaatc aagctctagc cgacttagct
     4141 ggccagggag tgatatcctc tgagctggct atgggactca caacgcttcc cgatgagtta
     4201 tcaaagttat tagaacgcac gggccgggat cggtctgggg ggatgtcgtt ggccaggttg
     4261 cgcaatcgga tgtagatgtc aatgattgct ttttggatct tcttcagaga aagcgagcat
     4321 taattcgaaa tgccgagatt tgaatatcgt gcgaaaaact tgaaaggaca ggcagttcat
     4381 ggggaagtgt tggctggcac ttccagtgag gccttacaac ttcttcgtag gcaggccgtc
     4441 ctggtgacca gtctccagga aaaagtcgaa aaggtattca atctcagttg cctggtgacc
     4501 ggctggaacc gtcaggggac ttggcgagcc gtgagaagta aagaattggt ggtctttacc
     4561 catcaattgg caaccttgat tcgagcggga gtgcctcttt tggaatgcct ggatattctt
     4621 tccacagaag ctgaaagcac gaccttgcaa caggttgtca agcatattcg agaggatgtg
     4681 gaggggggaa ccttgttggc tcatgcttta aagcgccatc caaccgtctt ccatgaattt
     4741 taccggagta tggttgaagt gggggagacg acgggtcgtt tggatgaaag tttgactcaa
     4801 cttgccgtgt atcttggtaa gcaggctcag ttacgggcga aaattttttc tggcttagcc
     4861 tatccggctt tgctcgtaac tgtcgcgatg attgtcctgg tctttttgct gatttgggtt
     4921 gtccctcttt tttctggctt attccaagat atgggtgaat cccttccctg gttgacgcaa
     4981 gtggtgattg acgtggctga aggggttaga gattactttt ttctgttgac gattttcgtg
     5041 ggaagtttag taatgggtat ccggtggatg ctgaaaaatc cgaaaagtcg gcaggccttt
     5101 gatgggtggg tcttacgggt ccctcttctg ggatccgtga tccaaaaggc ggcaacggtg
     5161 cgcttttcca ggaccttagg gtttttagtc cgtcgtgggg tcccgttgtt gtctgccttg
     5221 ggtgtggcag gaacggtgac cggtaataag gtatttgagc aaagtatcaa gctggcgact
     5281 acagcagttc aagatggaat gcccatttcc gaggccttga gggcacgcca ggttttcccg
     5341 cccatggttc cccaaatgct caaagtggga gaatcaaccg gttcaattga tgttatgctg
     5401 gaaaaaattg cggatttctt tgagcaagag gtcgatcgaa cggtagccac tttaacgtcg
     5461 gtgttggagc cctttattat cttggtcgtg ggctgtggga ttgctcttgt cgtggtggcc
     5521 atgtatcttc ccattttttc catcggatca gttattggat aaatttgaag gtatttcgtc
     5581 gtgagtggga gtgctatgtc agttactgag atcgtcgtga gggcgccggc aaaagtaaat
     5641 ctattgattc gggtattgga tcaattgccc aatgggtatc ataatttgtg gtctctcatg
     5701 catacggtcg atgtctttga tctcctccga atcagacttc actctgatcg ggagggcctt
     5761 gccttgacgt gtggagatgc cccgttgccc ttggataagg gaaatctggt ttatcgggcg
     5821 gctgaattgg tccttcagcg ggctgaaaag actgtagggg ttgatattga gctaaccaaa
     5881 gtcattccct tgtcggctgg tcttggaggt gggagtagtg atgccgccgc cactttgtat
     5941 ggcctcgctc acttgctggg gttaaattgg actttatctg acttgtgtga ggcgggtgca
     6001 acactcggaa gtgatattcc cttttttttt agagcacctt gtgcagtggt tcgtgggtgg
     6061 ggccaggatg tggtggcctg ttccatccaa ggtgaacggt gggtggttct tgtcaatccc
     6121 ggctttccta ttcagacaaa atgggcatat aaacagttgg cctctcaacg ggatgctgtt
     6181 cgtcctttag atgactctac aaagaagata gatcgtgagt tgagcctctc ctgggaggaa
     6241 gtgatcggga cgatggagaa tgattttgag gcgccgttgt tcccgtttta tcccattttg
     6301 ggatttatta aagatacttt gctttccttt ggggctcaag ccgcactatt atcaggtagt
     6361 ggagccaccg t
//
LOCUS       DAORAQ010000150         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1825_length_6355_cov_74.409841, whole genome shotgun sequence.
ACCESSION   DAORAQ010000150 DAORAQ010000000
VERSION     DAORAQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_6355_cov_74.409841"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKM72_11335"
     CDS             complement(<1..437)
                     /locus_tag="PKM72_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61424.1"
                     /translation="MAKELPVLPSPKQSETPEKEELTYSRVFCTREDSPPLKLLLDFL
                     KSKNQVPLIPKMDPAALEDWDWVHISLGYSRERKPIQLFCVRNRGTYQDVFEGEKTSF
                     FNRITVFDDVEAEIARECISTARFIATTQMVKKDMTDEGYDFNG"
     gene            772..1062
                     /locus_tag="PKM72_11340"
     CDS             772..1062
                     /locus_tag="PKM72_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNP61425.1"
                     /translation="MGKAMTKSQIADHLAKKADVTKKIATQILDDFAALAYKEAKNAF
                     VVPGVGKLVLAHRKARMGRNPQTGAAIKIPAKRVVKFRVAKAAKDAILGVKK"
     gene            1524..2285
                     /locus_tag="PKM72_11345"
     CDS             1524..2285
                     /locus_tag="PKM72_11345"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61426.1"
                     /translation="MASAASHLGEIFLHRGNLSKAHECYEQATEEARQLGSPKALSDA
                     LGNLGNVSALLERYEQAEACYREVLEIQRTLQEAHAIGETLVNLGSLKADRGEPETAH
                     AFYLEAIDYLTPLGHDRALGILYSNLALQELHLHQSEPAIQAFHRALDYHRKVGNEDG
                     LATTYGQLGKTFLHLGHLERAEACLNNATEHFIKLGNTTGEAGALRLLADVYLERKDT
                     ISALRCLERVVQIDLTYRLPQYEDDRQRLACLRDS"
     gene            2742..4151
                     /gene="ppnN"
                     /locus_tag="PKM72_11350"
     CDS             2742..4151
                     /gene="ppnN"
                     /locus_tag="PKM72_11350"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HNP61427.1"
                     /translation="MENNREYITSKRTSSGQGVSVSITPDTAMHMLSQHEVEKLRETS
                     EGGLHELFRSSALAVLTSGNDTDDTKAFFARYADFDIALEQRDRGVKLELINAPASAF
                     VDGEIIQGIKEHLFSVLRDIIYVHNEIHHNKKFDLTTSNGITNAIFHILRNAEIIHSV
                     EDPHLVVCWGGHSISEVEYRYTKEVGHQLGLRSMDICTGCGPGAMKGPMKGATIAHAK
                     QRIKNGRYLGVTEPGIITAEAPNPIVNELVIMPDIEKRLEAFVRVGHGFVVFPGGAGT
                     LEEILYLLGVLLHPDNNEVPFPLIFTGPQGSASYFETIDTFIKQTLGLHAAQRYQVII
                     DDPQRGAYEITTGIQRVREFRKRTHDAFYFNWHLTIDHEFQKPFEPTHESMAKLNLHA
                     DQPIHMLAANLRRAFSGIVAGHVKPQVLNAIEKNGPFKIHGDSTIMKLMDELLQSFIN
                     QHRMKIDQQNFKPCYEIIA"
     gene            4932..5339
                     /locus_tag="PKM72_11355"
     CDS             4932..5339
                     /locus_tag="PKM72_11355"
                     /inference="COORDINATES: protein motif:HMM:NF025140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HNP61428.1"
                     /translation="MKSIAMRLQKLGVLLVVPSVLGLGFLSVANAQNVNSQSQGVESQ
                     PAQKELIEPNLQPFAGAYKEISQIHSTYKERIMQADDPSKTESLQQEANEKMSQAVTD
                     HGLTISDYNAIFQSIQNDPALKEEFMTVLNRTQ"
     gene            5379..6092
                     /locus_tag="PKM72_11360"
     CDS             5379..6092
                     /locus_tag="PKM72_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61429.1"
                     /translation="MESLLKNGNRFFMWLNDGKGRPLIWEEQMAVMEQVVGRAAILIL
                     LLLVIGGCSNATTDTASEEPKANQSGFTASVSGSVDGEVSGAGVATYLPPKERDPVTG
                     SRPGYFLVANLNSDGTEEREFLFIFRIPDKAEPGDYDLVTPNPLNVGENFDVQVEMVE
                     KGESISYQTNTVGTITLENFAPEPTDSGNSNITGTFQFVTENSEGDQISATGTFDFPL
                     GRKMVSQYSGYSVKEVFRG"
BASE COUNT         1720 a         1391 c         1525 g         1719 t
ORIGIN      
        1 ccgttaaaat catagccctc atcagtcata tcttttttca ccatttgggt ggtggcgatg
       61 aagcgagctg ttgaaataca ttctcgggca atttcagcct cgacatcatc aaagacagtt
      121 atacgattga aaaaagaggt tttttctcct tcaaacacgt cctgataggt gccacggttg
      181 cgcacgcaaa ataactgaat gggtttcctc tcccggctat aaccgaggga tatatgaacc
      241 caatcccaat cctcaagggc ggccggatcc attttgggaa tcagcggaac ctggttcttg
      301 gatttgagaa agtctaaaag caacttcagt ggtgggctat cttcccgcgt gcaaaacacc
      361 cgtgaatacg tcagttcttc cttttcggga gtttctgatt gtttggggga ggggagaaca
      421 ggtaactctt ttgccatgat caatgcctcg tgagtgttgt gccaattgca cgtatgacta
      481 aaataggtaa aatgtgtatt ctcgctactt taccgtggta aaagccgagg cagcaaggca
      541 gaagaatcat ttgagaaatt atggtaaaag aatattcccc gcactgggct tgttttttgt
      601 tgacagtttt tgagccattc ggtagactct ggcgccgttg tgacagaggg gaaaggtgtt
      661 cctgcgttaa actctttctc caaaatgaca gacaaggcaa tccttccttg gaattttaga
      721 atcaacaaat ggtgtctctt actaaacaat tcaattgagg aggaaatgtg tatgggaaag
      781 gccatgacaa agtcccagat tgccgatcac ctggctaaaa aagcggatgt caccaagaaa
      841 attgcgacgc aaattcttga tgattttgcg gctttggctt ataaagaagc caaaaatgcg
      901 tttgtagtac ctggtgttgg caagctcgta cttgcgcacc gaaaagccag aatgggcaga
      961 aatccccaaa ctggagccgc gattaaaatc ccagccaagc gggtagtgaa attccgagtg
     1021 gccaaagcgg caaaagatgc gatcttgggg gttaaaaagt aactgtacac ttcccaagga
     1081 attatcctgt cggcttttcc ctctctaagc cggttggttt aaatgaatgt aggggaaggc
     1141 cacacctttc ccattagaat gtccttcaac agccgagtcc agacatctgg actcggctgt
     1201 tgttttatga ggctgaatgg gctctataaa gactcaaagg ggatgggaca cctttcaggc
     1261 tgaatgtcgc aactgccctt ctcccaaaag cttacatctg ggattttcat gatcaagatg
     1321 gtattcaggc catagaatgt tgctactcct cgatccggtg ataccttcca gctctttgtc
     1381 agagaaaaag tgcattcttg ccaaggtgtg gctccgggta taggcttacg gcaggagcaa
     1441 gcatagtgag tcgttgtcgg atggatgttt taccgaattt tgtctgagaa ctgaacaatt
     1501 gtcacgggat tttctttaac atcatggcca gcgctgcttc gcatctcggc gaaatttttc
     1561 tgcatcgcgg gaatctttca aaagcgcatg agtgctatga acaggctacc gaggaggcaa
     1621 ggcaattggg atctcccaag gccttgtcag atgcattggg caatctggga aatgtcagcg
     1681 ccttgttaga acgctatgag caagccgagg cctgttatcg agaagttctg gaaattcaac
     1741 gaaccttgca agaagcgcat gctatcgggg aaacactggt gaatttagga agccttaaag
     1801 ccgacagggg agagccggaa acggcgcacg cattttattt ggaagctatt gattatttaa
     1861 cccctttagg ccatgaccgg gcgttgggta ttttatatag caatctggcc ctgcaggaat
     1921 tgcatttaca tcagtccgaa cctgccattc aagcctttca cagggcttta gactaccatc
     1981 gcaaagtggg gaatgaagac ggcttggcta ctacctatgg gcaacttggg aaaacctttc
     2041 ttcatctggg ccaccttgaa cgggccgagg cctgtctcaa taatgcgact gaacatttca
     2101 tcaagctagg aaataccacg ggagaggcag gggccttgcg tctattggct gacgtctatc
     2161 tggagcgaaa agataccatc tcagcacttc gctgtctgga gcgtgtggtg caaatcgatc
     2221 tgacctatcg tcttccacaa tatgaagatg atcgtcaacg attagcctgt ttgcgtgatt
     2281 cctaagtgga ctttcctgaa taatagtctt catgctggtg gaatgtgttt gcggaaattc
     2341 ccaagctttt cacgagtctg taatgagagg tcatcacccc ataaaggagt gaaaattatt
     2401 tgttctggtt tccaattgaa cttagaatcc tgccagcact atcctgactt ttttcaactc
     2461 tggacgcggc tccggcgaaa tccttggcgt taggttgggc cgtaaagttc ggtccagaat
     2521 ttccaggcca tgagcccgtg cctcaagggc agaaccagct tctttacgta atcgtagcac
     2581 cgtatatttt gccggatgtg ttcccattcc atttgtgcat tagggcgtgt tctgacaccg
     2641 accatagagc ctgaacacgt cggtgtgata aattgacctc ctcctggggg ccagtatttg
     2701 gccccgttca cacacaaagg gctaggagct agagcgtaag aatggaaaac aatcgagagt
     2761 acataacgtc gaagcgaaca tcctcagggc agggcgtgag tgtgagcatc acccccgata
     2821 cggctatgca tatgctgtcg cagcacgaag tggagaagct cagggaaacc agtgagggcg
     2881 gcttgcatga actattcaga agtagtgccc tggcggtcct aacgagtggg aatgatacgg
     2941 acgacacgaa agcatttttt gcccgctatg cagatttcga cattgccttg gagcaaaggg
     3001 atcgcggagt gaagcttgaa ctcatcaatg cccctgccag cgcgtttgtg gatggggaaa
     3061 ttattcaagg aattaaagag catctctttt cggtgctacg agacattatt tatgtccata
     3121 acgaaataca ccacaataaa aaattcgatt taaccacctc caatgggatc accaacgcga
     3181 ttttccatat cctacgaaat gcggaaatca ttcattcggt ggaggatcca catttggtgg
     3241 tgtgctgggg aggccattca atcagcgagg tcgaatatcg ctacacgaaa gaagtcgggc
     3301 atcaactcgg tttacgttcc atggatattt gcacgggttg tggtccaggg gctatgaagg
     3361 gaccgatgaa aggagcaacg atcgctcacg cgaaacaacg tattaaaaat ggtcgatatt
     3421 tgggcgtcac ggagccgggt atcattacgg ctgaagctcc gaatcccatt gtaaatgaat
     3481 tggtgattat gccggatatt gaaaaacgtc ttgaagcgtt tgttcgtgtt ggacatgggt
     3541 ttgtcgtgtt tcccggcggt gcggggactt tggaggaaat tctctatctc ctcggtgttc
     3601 tactacatcc tgacaataat gaggtgcctt tcccgcttat ttttacgggc ccccaaggca
     3661 gtgccagcta ttttgaaacc attgatacct ttatcaagca gacactcgga cttcatgcgg
     3721 cgcaacggta tcaagtcatc attgatgatc ctcagcgtgg agcctatgaa ataacgacag
     3781 gcatacaacg tgtacgggaa tttcgaaaac ggacacatga cgccttctat ttcaactggc
     3841 acctgaccat cgaccatgaa tttcaaaaac catttgagcc gacacatgag tcgatggcga
     3901 aattgaatct ccatgccgac caaccgattc atatgttggc tgcgaatttg cgtcgggcat
     3961 tttcaggaat tgttgccggc catgtgaagc cacaagtgct caatgccatt gaaaaaaatg
     4021 ggccatttaa aattcacggg gattcaacga ttatgaaact catggatgaa ctcctgcaat
     4081 ccttcattaa ccaacaccgg atgaaaattg atcaacagaa tttcaagcct tgttatgaaa
     4141 ttatcgccta gtcctcggat ggcatctttt gttttcgaga acagcgttct ccccccatct
     4201 ctttaattga agttgggctt tccgctttcc cgcaatacca aattgtagaa ttctacccaa
     4261 catcatcttc atggtttttc gcaattgtca gcttaatctt gccttttaat attccggcgc
     4321 ttgtagacgt gattttttgc aacaaggcat cgagcatggt cccccggtct tagtattccc
     4381 tttgacctgt atagactctt ccttggattt cccctgccaa ttaaagaatc aaatcaaagg
     4441 tgctcccttt acaaaagcaa aatgacgaag tcaatggata aggtattgac tcttaatccc
     4501 aatacatcca atcccaaatg taggcctccc tcgtttattc tcaatagagt gtaggtatat
     4561 cgtttgtata cgtagggtct tccctacgct atgtctggaa aaagcaggat atgcgaaaag
     4621 gaagttttag ggccggattc tgcgcttatg ccttatgtta tgccctgtag gttggtactt
     4681 ctgtgccttg tgcggacatt ttggcctggc ttttgcgtga caagtaaaat agggatgaaa
     4741 aataccgtca tgaaaaagat tacgttggcc cgattctatc catgtcgatc ctaaaggaga
     4801 ttcaaatttt ctggacttat tgcttgagtt tgcaccgtgg ccgtttcgga aaaaaataaa
     4861 aaaacatgta ggtttccttg gtctcagctt gctgaggaat attaatttta atttaaaaag
     4921 ggagaaatgg tatgaaatcc atagcaatga ggttacagaa gttgggtgtg ctgctagtgg
     4981 tcccaagtgt ccttggattg ggatttcttt ctgtcgcgaa tgcgcaaaat gttaattccc
     5041 agtcacaggg agttgaatcc caacctgcgc agaaagaatt gattgagccg aatctccaac
     5101 cttttgctgg ggcttacaag gaaatctccc agattcattc gacatacaaa gagcgaatta
     5161 tgcaagcaga tgacccaagc aaaacggaat ctctccaaca agaggctaac gagaagatga
     5221 gccaggccgt cacggatcat ggcctgacca tttcggacta caatgctatt tttcaatcaa
     5281 ttcaaaatga cccggccctc aaggaagaat tcatgacggt actgaatcga actcagtaaa
     5341 tgaattactc atccccaccc tcacgcttta tggaaggaat ggaatcttta ttaaaaaacg
     5401 gaaaccgttt tttcatgtgg ttaaatgatg ggaaggggag accacttata tgggaggaac
     5461 agatggcagt aatggagcaa gtggttggaa gagcagcaat tttaatttta ctactacttg
     5521 tcatcggtgg gtgttctaat gcaaccactg acaccgcttc cgaagagcct aaggctaatc
     5581 agtcgggatt tactgcaagc gtatccggct ctgttgatgg agaagtatcc ggggctggtg
     5641 tcgctacgta ccttcccccg aaagagcgag atccagtaac cgggagtcgt cccggttact
     5701 ttttggtggc gaaccttaat tcagatggga cggaggaaag agaatttctt tttatcttcc
     5761 gaatccctga taaggcagag cctggcgatt atgacctggt gacgcccaat cctttgaatg
     5821 tgggtgaaaa ctttgatgtg caggtcgaaa tggtggaaaa aggagaatcc atttcctacc
     5881 aaaccaatac cgtgggaacc atcactttag aaaattttgc tcctgaacct actgattcag
     5941 gaaatagtaa tattaccgga acatttcaat ttgtcaccga gaatagtgaa ggggaccaga
     6001 tttcagccac tggcacattt gatttcccct tgggaagaaa aatggtatct cagtattcag
     6061 gctattccgt gaaggaagtg ttcaggggat gaaagaaaac tccataatag cctgttaaaa
     6121 tagtgtggag acctgagggc gatcctgatc tcaaatctat ttctggtgga cgttctccaa
     6181 agcgagagga ggattgcgtt cgatccacag aatgttaagt tactaaattc tttcgtgatc
     6241 tcccgcgtcc tttgccattt gtttaataaa aatttctcgt tgtgatcgat ctctcctatc
     6301 cgaggttcct atccgcttca tccctagatt ttatttgatc attttggcac agttt
//
LOCUS       DAORAQ010000151         6181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1869_length_6181_cov_58.627000, whole genome shotgun sequence.
ACCESSION   DAORAQ010000151 DAORAQ010000000
VERSION     DAORAQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_6181_cov_58.627000"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKM72_11365"
     CDS             complement(<1..322)
                     /locus_tag="PKM72_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61430.1"
                     /translation="MSSAALMTSNGSVKTQSLNARSLTAPTTIEETGVPEELLIQLLL
                     RTLYTQGELTERSAGEIMKLPYGVMKELFTLIQREKLCEVKGHGESLGVLLRYVLTEA
                     GRQRA"
     gene            583..2133
                     /locus_tag="PKM72_11370"
     CDS             583..2133
                     /locus_tag="PKM72_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNP61431.1"
                     /translation="MGGLSKFQLSWGKWLLVFSFLSFVGCSESLPDYTLRFGLANAPT
                     NLDPRYATDATSARLNRLLYSRLVDFGEGTIPIPALATWRKLSPTHYRFDLQEPKPLF
                     PNGTQLSAYDVKATYASILNPVMASPHRGILQIITRIDTPTENTVDFFLKQPDALFPG
                     YLVIGILPANLIANGHPFHDHPMGSGAFQFLDRPDDTRLIIERRADGQRVEFVRIPDP
                     TVRTLKLLANEIHLVQNDLPPELVTYLSAHEGITLQQRQGANFAYLGFNQLDPVVGQE
                     LVRKAIAHAIDREHIIQFVLRGRARVAQALFPPEHWVGHASLPGYTYDPAKAKRLLKE
                     AGYDHDHRPTITYKTSTDPFRLRLATIIQYQLDQVGIDVSIQSHDWGTFYGDIKSGRF
                     QMYSLAWVGVKSPDIFRYAFHSDATPPVGANRGHYADALADRLMTQAEQTLNKQEQQT
                     IYRSLQARLLDTLPYVPLWFEDHYALSSPTITGYQLNADGNFDGLNQVRWLGNPSVPP
                     KYLARLYP"
     gene            2198..3124
                     /locus_tag="PKM72_11375"
     CDS             2198..3124
                     /locus_tag="PKM72_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP61432.1"
                     /translation="MTHFLVTRILSAMLAILGVICLVFLLIHLIPGDPVEVMLGESAQ
                     PTDKESLRHALGLDLPLHQQWWIYFKGLLHLDLGTSLFSGRAIVDLLIERIPATLYLS
                     LVSLLVAIALALPLGLVAAVRQHTPLDYGAMGFALFGMSIPNFWMGPLLILVGALWLG
                     WFPVSGKEGWNSVVLPALTLGTAMAAILARMIRSSVLEVLGEDFMRTARAKGLSSTRA
                     VLHHALPNALLPILTLLGLQLGGLLGGAVITETVFAWPGLGLLMIEAIQQRDYPVVQA
                     AVLCISVTYIVVNLLTDLLYAWLDPRIQFTTD"
     gene            3127..3936
                     /locus_tag="PKM72_11380"
     CDS             3127..3936
                     /locus_tag="PKM72_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP61433.1"
                     /translation="MRLWLPLSIIASWSFLAICLPLFSLTPDAIDLTNILAPPSLEHW
                     LGHDELGRPVADRLISGAQTSFFVSLCVVALGVSLGGILGVSSAYLSGWVDAVFVRMV
                     DIMLAFPGLLLAIALAGALGPGIGNVILALGIVGWVGYARLARGQVLALKHADHVLAA
                     QAIGAPPWRIIFHHLLPLIMAPLIVEASFGIAGAIIAEAGLSFLGLGVQPPAASWGSM
                     IRDGTRYMLVAPHLVIFPGLTLMAVVLAANMLGDWLRDRLDVKEQTSRRQS"
     gene            complement(4038..4670)
                     /locus_tag="PKM72_11385"
     CDS             complement(4038..4670)
                     /locus_tag="PKM72_11385"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNP61434.1"
                     /translation="MRIRMLFLGVLLGAIVLSGSWGKIQAAEEAESEVRMAPGTEVNF
                     SPEVVAGYIHAVIAADRALYTTHVVDRMQENRIVIAAEAWKQRKALPLPAQMLLMGGK
                     VAEMGGSGLRYRLASLWPIYEENGPSTDFEEAGLKSVAENPDEVYSGIIKRGDQRFFK
                     AIFADRAVSKACVECHNGHLLSSKHDFKLGDVMGGIIISFPLPSEKKETH"
     gene            complement(4830..5750)
                     /locus_tag="PKM72_11390"
     CDS             complement(4830..5750)
                     /locus_tag="PKM72_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNP61435.1"
                     /translation="MKGRMICNGIVGVGLIALIAQTAWSYDEITVTNGGTIQGKVTIT
                     GEKPRPMAFNLVTIPDPVFCGTISTGTGWRIVEDFIIGPDKSLKDVVVYLKEIEKGKP
                     FHLPQVRIESVDCDFIPFVNVVRDQDKIEVVNMDPVEHDIQGYETARERGARVLFNRP
                     LPMNPYHKVAGIFGKKHLPGEPMIEKIHLRKGRNVFVMQCGFHPYMFSWGLVLENPYY
                     AITPEDGTFKITNVPPGDYVLTAWHPGMKEFVEQQVTVTAQKATEANFQYEAPQGRRS
                     VHEIEENPRFGLELLEEGLEIVPSVRRQIP"
     gene            complement(5889..>6181)
                     /locus_tag="PKM72_11395"
     CDS             complement(5889..>6181)
                     /locus_tag="PKM72_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61436.1"
                     /translation="EPFLPGADMISTVEFSGSEGLDVFLPSAGGKWSMTGDYVWSNGR
                     LPYSQSGQWGYLRVLPSTDQRIQPLDGSAMSSKQAALEEPNGEPRIIPTVAR"
BASE COUNT         1361 a         1801 c         1433 g         1586 t
ORIGIN      
        1 tggctcgctg acgacccgct tccgtcaaca cataccggag taacacccca agcgattcgc
       61 catgcccctt gacctcgcag agcttctccc gttgaatgag ggtgaacaac tccttcataa
      121 ccccataggg caatttcata atctccccgg ctgatcgctc tgtgagttcc ccctgagtgt
      181 agagggtcct gagcagcagc tggatgagca attcctccgg cacaccggtc tcctcaatcg
      241 tcgtcggagc cgttaaagag cgagcattga gagattgagt cttcacactt ccattagatg
      301 tcatcaaggc agccgaactc attcgtaatc tcctttgatt agtccatacc tggaacaaga
      361 ggacatgacg cggcagaagt tcttcgagtc gtcgatattc gcccttttac aaaagggata
      421 atctctgtaa aattaagaca acatcgccat gcatacgtcc ccacgctatt tctcaatctc
      481 gcgcccacca ctgttttctg agctttcatg aggacttctt tccacgactc ttgcgatccc
      541 agccctgtgt gttttgctgt gcgttcccct atgacttatt tcatgggggg gctttctaag
      601 tttcaattga gctggggaaa gtggctgttg gttttcagct tcttatcatt tgtgggctgt
      661 tccgaatccc ttcccgatta cacccttcgg tttggattag ccaatgctcc cacgaattta
      721 gatccgcgtt acgccactga tgcgacttcg gctcgactca atcggctcct gtattcgcgc
      781 ttagtggact tcggcgaggg cacgattccg attccagctc tggccacatg gcgtaaactc
      841 tctcccaccc attatcgatt cgacttacag gaaccgaaac cgctctttcc caatggcacc
      901 caactctccg cctacgatgt caaagccacc tatgcttcca ttttgaatcc tgtcatggcc
      961 tcacctcatc gcggtatttt gcaaataatt acccggatcg acaccccaac cgagaatacc
     1021 gtcgatttct tcttgaagca gcccgatgcg ttatttcccg gctacttagt tatcggcatt
     1081 ctcccggcaa acctaattgc caatgggcat ccattccatg accatcccat gggaagcggg
     1141 gcctttcaat ttctcgatcg gcccgacgac acccggctta tcatcgagcg acgagccgat
     1201 ggacaacgag ttgagtttgt cagaattccc gatccgaccg ttcgaacact caagcttctg
     1261 gccaatgaga ttcatttggt tcagaatgat ctcccccctg aactcgtcac ctatttgtct
     1321 gcccacgagg gcatcacctt gcaacagcgt cagggggcaa actttgccta cctgggtttc
     1381 aatcaattag atcctgtggt cggccaggag ttagtgcgaa aggccattgc ccatgccatt
     1441 gatcgtgagc acatcattca attcgttctg aggggacgag ccagagtcgc gcaggcgctc
     1501 tttcccccgg aacattgggt tgggcatgct tccttaccgg gatacacata cgatcctgcg
     1561 aaggctaaac gcctattgaa agaagccgga tatgatcatg accatcggcc caccattacc
     1621 tataaaacct ctaccgaccc ttttcggtta cgcttggcga cgatcattca ataccaactc
     1681 gaccaggttg gaattgatgt ctcgatccaa agccatgatt ggggcacatt ttacggtgac
     1741 atcaagtccg gacgattcca aatgtacagt ctggcctggg tgggcgtgaa atctccggat
     1801 atttttcgat atgcctttca cagtgacgcc actcctcctg tcggtgctaa tcgtggtcac
     1861 tatgccgatg ccttggccga tcgtctcatg acccaagccg aacagaccct gaacaaacag
     1921 gaacagcaga cgatctaccg cagccttcaa gcccgtctgt tggacaccct cccctatgtg
     1981 cctctctggt ttgaagacca ttatgcctta tcgtccccta ccatcaccgg atatcaattg
     2041 aacgctgatg gcaattttga cggactcaat caggtccggt ggttgggaaa tccctcagtt
     2101 ccccccaaat acctagcacg gctttaccca tgacacggtg ccataaccag gtcggggaat
     2161 cttctttttt ccaattagcg ccttttccaa tctcgtcatg actcatttcc tggtgaccag
     2221 aatcctgagc gcgatgctcg ccatcctggg agtgatttgc ctggtttttc tgttgattca
     2281 tcttattcca ggcgaccccg tagaagtgat gttgggtgaa tcggctcaac cgacagacaa
     2341 ggaaagctta cgccatgcct taggcttgga tctccctctc catcaacagt ggtggatcta
     2401 tttcaaggga ctcctgcatt tggatttggg cacctcactt ttttcaggtc gggcaattgt
     2461 agatctgctc attgaacgca ttcccgccac cctctactta agtctcgtct ccctgcttgt
     2521 ggcaatcgcc ttggcgctac cccttggcct cgtcgcggcc gtgcgccaac atactcctct
     2581 ggattatggt gccatggggt ttgcgttgtt cggcatgtca attcctaatt tctggatggg
     2641 tcctttactc attttagtcg gcgcactctg gctgggctgg tttcccgtga gtgggaaaga
     2701 gggctggaac tcagtcgtgc tgcctgccct gacactcggc acggccatgg cggccatcct
     2761 ggccagaatg atccggagca gtgttcttga ggtattgggg gaggacttca tgcgaaccgc
     2821 ccgcgccaaa ggcctctcct ccacccgggc ggtcttgcac catgcactcc ccaatgccct
     2881 cttacccatc ctgactctcc tgggccttca gctcggggga ttgcttggtg gagcggtgat
     2941 cactgaaacg gtgtttgcct ggccgggatt aggccttctg atgattgaag ccattcaaca
     3001 acgggactat ccggtcgtgc aagcggcggt gctctgtatc agcgtgactt atatcgtcgt
     3061 gaatctgctg actgatttgc tgtatgcctg gttggaccct cgcatccaat tcaccaccga
     3121 ttaaccatgc gtctttggct cccactttcc atcatcgcct catggtcctt cctggccatt
     3181 tgccttccct tgttttcctt aacccctgat gcgattgatc tcaccaatat ccttgccccg
     3241 ccaagcctgg agcactggtt aggacatgat gaattgggca gaccggttgc cgaccgcctg
     3301 atcagcggcg cacaaacgtc gttttttgtc tctctgtgcg tggtcgccct gggggtcagt
     3361 cttggaggca ttctcggcgt ctccagtgcc tatctcagcg gatgggtcga tgcggtcttt
     3421 gtacgaatgg tggacatcat gctcgccttc cccgggttac tgctggccat cgccctcgcc
     3481 ggagccttgg gaccgggaat cgggaatgtg attctggctt tgggaatcgt gggatgggtg
     3541 ggatatgccc gactggcgag aggacaggtg cttgccctca aacacgccga tcacgtgctg
     3601 gccgcccaag ctatcggcgc gccgccctgg cgaattattt tccaccacct tctgccgtta
     3661 atcatggccc cgctcattgt tgaagccagc ttcggtatcg ccggggccat catcgccgaa
     3721 gcaggccttt cgtttttggg gctgggggtt caacctcctg ccgcatcatg gggcagcatg
     3781 atccgcgacg ggacccgcta tatgttggtg gccccccacc tggtcatctt cccgggtttg
     3841 accctcatgg cggtcgttct cgcggcaaac atgttggggg attggctgag agatcggctg
     3901 gatgtgaaag aacagacctc acgaagacaa tcatgaaaca ggtcggacat caggcagaca
     3961 aacggtgact gcgggatcaa ctcccacagc caccgtcttg atttcggatg taataatcga
     4021 ggaacggtcc gtgatcgtta gtgtgtttct tttttttctg agggaagtgg aaacgaaatg
     4081 atgatgccgc ccatgacatc acccaactta aagtcatgtt tagaactcaa caaatgtccg
     4141 ttgtggcact ctacacaagc tttagaaaca gcccgatccg caaagatggc tttaaaaaac
     4201 cgttgatctc ctctcttgat aattccacta tagacctcat caggattctc ggccacagat
     4261 tttaatccgg cctcttcgaa atccgtactc ggcccgttct cctcataaat aggccacagg
     4321 ctggccagac gataccgcag gccggatccg cccatttcgg ccactttgcc acccatcaag
     4381 agcatctgcg ccgggagcgg aagcgccttc cgttgcttcc aagcttctgc ggcaatcacg
     4441 attcgattct cctgcatgcg atccacgaca tgagtcgtat agagtgcccg gtctgcggca
     4501 atcaccgcat ggatataccc ggccacaact tccggagaga aattcacttc agtgcctggg
     4561 gccattcgca cttccgattc agcctcctcg gcagcctgaa tttttcccca actgccggag
     4621 agcacgattg cccccaacag aactcctaag aacaacatcc gtattcgcat gatggcctcc
     4681 ttttgtccgt catcacttca gtaaatgaat caacatcctg ttgccttgct tcgatcggac
     4741 tccctcagga gacttctcct gttcgtggac tatctgtcat caatttcccc atttgccgaa
     4801 tgaccgctca gggcacacaa aacaggccgc tagggaatct ggcgtctaac cgaaggcacg
     4861 atttcaagcc cttcttccaa taactccaat ccaaacctgg gattttcctc aatttcatgc
     4921 acactacgcc gaccttgtgg agcctcatat tgaaaattgg cctcggtggc cttctgagcc
     4981 gtcactgtga cctgttgctc cacaaactcc ttcatcccgg gatgccaggc agtcaacaca
     5041 tagtctccag ggggaacatt ggtaatttta aatgttccat cctccggggt gatggcataa
     5101 tacgggtttt caagcaccaa tccccaggaa aacatatagg gatgaaatcc gcactgcatc
     5161 acaaacacat tccgtccctt tcgtaaatga attttttcga tcatcggttc gccgggaaga
     5221 tgtttcttgc caaatattcc tgccacctta tgatacgggt tcatcggtaa gggacgattg
     5281 aacaatactc tggcccctcg ttctctggcc gtttcataac cctgaatatc atgctcaacc
     5341 ggatccatat tcacgacctc aattttgtcc tggtcacgga ccacattcac aaagggaata
     5401 aaatcacagt cgactgattc gatcctgacc tggggcagat gaaacggttt ccctttttca
     5461 atctctttga gataaaccac cacatctttg aggcttttat caggcccgat aataaaatct
     5521 tcaacaattc tccaacctgt ccccgtggaa atagttccac aaaacaccgg atcgggaatg
     5581 gtcaccagat tgaaagccat gggtctgggc ttttcccccg taatggtcac cttcccctga
     5641 atggttcccc cattagtcac ggtaatttca tcataggacc aagccgtttg cgcgataaga
     5701 gcaatcagcc caacacctac gattccatta catatcattc ggcccttcat gcttccctcc
     5761 tcaaaataaa ataaatgaaa taatgggcat cctctctgtc attctatcga accatttact
     5821 caaaaaaaag cggggaagcc gaagtgtggc ttccccgctt tcgctaaccc tcggaatgag
     5881 agtccagctt acctcgcaac ggtaggaata attcgcggtt cgccattggg ctcctcgaga
     5941 gccgcttgtt tggaagacat agccgaacca tccaatggct gaatccgttg atccgtgctc
     6001 ggcaacaccc gcagatagcc ccattgtccc gattgtgaat agggtaaccg gccattgctc
     6061 cacacataat ctccggtcat ggaccatttg ccaccagccg agggcaagaa gacatccaga
     6121 ccttctgaac ccgagaactc cacggtgcta atcatatccg caccgggcag gaagggttca
     6181 a
//
LOCUS       DAORAQ010000152         6165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1873_length_6165_cov_74.669231, whole genome shotgun sequence.
ACCESSION   DAORAQ010000152 DAORAQ010000000
VERSION     DAORAQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1873_length_6165_cov_74.669231"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..4063)
                     /locus_tag="PKM72_11400"
     CDS             complement(26..4063)
                     /locus_tag="PKM72_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP61437.1"
                     /translation="MEAARTTPPTILVVDDETDICLALSDLLGNEGYQVDSVETGNEA
                     LRRALLPHPYSAVILDLGLPDLDGLIVLQRLYSQDATLPVIILTAHGDQKEKIATLQH
                     HAFAHLIKPYDRHEVVEMVHRAVAVKNLAWKAVQAEEALTSSEAQRQVEQQRTHTLLS
                     ESERRLNLALKAGNMGIWDWHIPTNHLIWSEEVSRIFGLGSGKFDGTNEAYMNCVHFE
                     DRLLLGESIRRTLENDAPYEVEHRVVWPTGEVRWVACKGQVVEHQQGHPQRMLGTVQD
                     ITIRKQADIELRNSELFLNSIVENIPYTIFVKEAKNLRYLRFNKAGEALVGYPTETLI
                     GKTDYDFFSKSEADFFTGKDREVLASKTLLDVPEEVIHTKSHGTRYLHTKKIPLLDAN
                     GDPLYLLGISDDITAQKQAELERREREILLHLIFETGPGCIKRVAADGTLLHINPAGL
                     DLIDAEHASEVLGHSVFDLVVPEHLDAFKQMHQEVIQGKKRTLQFEVQSIKGTRIWIE
                     TYAVPFKNPITNEVEHLAVTHDITKRKQAELDLMERNRLLELDIEVTTVINQKETIPD
                     LLQGCTDALVRHLGAAFARIWCLDESDRVLKLCASSGLYTHLDGPHSRVPMGKFKIGL
                     IAAERKPILTNMVIGDPRVPDQEWAKQEGMMAFAGYPLVSNQEVLGVMGLFARHPLTE
                     FTLKSLGMVANRITVALERQVTKDEKNKLALFQQRLLASMEEGIYGLDLDGNATFVNP
                     AVLEMTGYEENELLGHSTHVLLHHSKPNGAPYSQEECLIHNSLRDGSVYHIDTEVFWR
                     KDGSSFPVEYVTTPIQNDQGKLEGTVVAFQDITQRKQAEKVLQESEERFRLLNEAIPQ
                     QVWTARADGSLDYVNQRVIEYFGCPFEEMIGDGWQRFLHPEDLPGCLERWEEARNTRQ
                     AYEIEFRLLRAKDKTFRWFLGRALPIFDQHDQVVKWFGTNTDITQLKELENQVRQSQK
                     MEAIGTLAGGIAHDFNNILMAIIGYTELAKQSARENVSAQKNLDEVLVAGQRAKELVQ
                     QILAFSRQTEQSRQPLDLQLVVKEVCKFLRATFPATIDIRQKFSGATSIILGDPIQMH
                     QVLMNLCANAEYAMRGGGGILELQLEHVTGEPDGIGTHPDLQGGSYVCLTVRDSGSGM
                     TPEVVQRIFDPFFTTKEVGEGTGMGLAVVHGIVISHGGVMKVESQPGQGAIFSLYFPE
                     MEAASYPGEDTPLQQEVFMGRGYILFVEDEEPLARLGEEAMKGLGYEVMVRTSSVEAL
                     EVFRADPHRFDAVVTDQTMPHITGEALARQLLELRPDVPIILCTGFSHSMTPEKAKAM
                     GIRAFLLKPLLIKDLARTLRDVLDS"
     gene            4440..5666
                     /locus_tag="PKM72_11405"
     CDS             4440..5666
                     /locus_tag="PKM72_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61438.1"
                     /translation="MTSTFGLWEFLSRDVTFLGGPHSPLLSWLGSGGILLFFLWHILK
                     MRKETASVQRAFERIHPKLQALANERRKMDRDRFTHHHGNGFSTHADRAASSSNRLDS
                     EDIQTLDAGMQQEPIFRSPWAQYRMTLILEQVPWFVDPRIFSTKPAAEVFTQETLIAK
                     HVNLTFYRQFPSLVTSLGLLLTFLALFIGLGKLHADGSEIVGIQGLINGLAGKFLTSI
                     VGLIVANIFSFLEKPIVARLMNAHHACLDLIDQLFPRKTMEQMLEQLTAKQGQSPKDP
                     SPSREENREHLLGWGTNGLIGPTADLTTAIQSLMTLQKEDHAEIRRTVSELPRVITDT
                     LHGPLNELTGAIHDLTILLKDIRPHRVQTETSFEERPPLWKPSANSRGPSMINFFDKI
                     GSWSKRPGLARHRRTG"
     gene            5675..>6165
                     /locus_tag="PKM72_11410"
     CDS             5675..>6165
                     /locus_tag="PKM72_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61439.1"
                     /translation="MDQDNASSPTTGGVIDLMTSLAMIFILLFAAFITQTSSEAQSEL
                     QEHKENVQAALRDHLERLGLSLDSDPQDPLTLLIVVPDNKLTFEFGQSILSHDAVQFV
                     QEAMPFYAAVLCGPLRDKIDSLAIQGHTDDRGDDAFNLKLSQERSLAVMVKGLEAIEA
                     QEPS"
BASE COUNT         1495 a         1693 c         1396 g         1581 t
ORIGIN      
        1 gaacgacaaa gtcacattcg gcacgtcatg aatcaagaac atcccgaaga gtccgcgcta
       61 gatccttgat caacagcggc tttaaaagga aagcacgaat gcccatggct ttggcctttt
      121 ccggggtcat actgtgacta aaccccgtac acagaataat aggcacgtcg ggtctgagtt
      181 ctaacaattg acgggcaaga gcttcgccgg ttatatgagg catcgtttga tcggttacaa
      241 ccgcatcaaa tcgatgggga tcagcccgaa agacttccag agcttcaacg ctgctcgtgc
      301 gtaccatgac ttcatacccc aatcccttca tggcctcctc cccaagcctg gcgagcggct
      361 cctcatcttc cacaaaaaga atataccctc ggcccataaa cacttcctgt tgcaatggcg
      421 tatcttcacc aggataactc gccgcctcca tctccgggaa atacaggctg aaaattgctc
      481 cctgtccagg ttgactctcc accttcatga cacccccatg actaatgacg attccatgga
      541 ccacagctaa tcccattccc gtcccttcac caacctcctt cgtggtaaag aagggatcaa
      601 agatgcgctg gaccacctcc ggcgtcatac ccgatccgga atctcgaacg gtcaggcata
      661 cataggaacc tccctgtagg tcaggatgtg tgccgatccc atcaggttca ccagtcacat
      721 gttccaactg caattcaaga atacctccgc ctccccgcat cgcgtactcg gcattggcac
      781 acaggttcat caacacttgg tgcatttgga taggatctcc taatatgatc gaggtagccc
      841 cactgaattt ttgccgaatg tcgatggttg ccgggaatgt cgctcggagg aatttgcaca
      901 cttctttgac caccaattga agatctagcg gctgccggct ctgctcggtt tgacgactaa
      961 atgccaatat ctgctgaacc aattccttgg cgcgctgccc ggcgaccaac acctcatcaa
     1021 gatttttctg ggcactgaca ttttccctgg cactctgttt agccagttcc gtatagccaa
     1081 tgattgccat aagaatatta ttgaaatcat gggcaatacc tcctgccagt gtcccaatcg
     1141 cctccatttt ttgggattgt cgtacctgat tttccaactc tttcaattga gtgatatcgg
     1201 tattcgtccc aaaccatttc acgacctgat catgttgatc gaaaatgggg agggctcgac
     1261 ccaaaaacca tcgaaatgtt ttatcttttg ctctcaggag tcgaaattca atttcatacg
     1321 cttggcgggt atttcgagct tcctcccacc gttccagaca tccaggcaga tcctccggat
     1381 gtagaaaccg ctgccaccca tccccgatca tctcctcaaa gggacagcca aagtattcga
     1441 taacacgttg attgacataa tcaagggaac catcagcccg agccgtccag acctgctgcg
     1501 gaatggcctc attgaggagc ctaaaacgct cttcactttc ttgaagaact ttttccgctt
     1561 gcttgcgttg ggtgatatct tgaaacgcga cgacggtccc ttccagcttt ccctgatcat
     1621 tttggatagg ggtcgtgaca tattcgaccg ggaagctcga tccatctttt cgccaaaata
     1681 cctctgtgtc gatatgatag acggagccat cccttaagga gttatggatt aggcattctt
     1741 cctgggaata gggggcgcca ttgggctttg agtgatggag aagcacatgc gtggagtgcc
     1801 caagcagttc gttctcctca taccccgtca tttctaatac cgccggattc acaaaagtcg
     1861 catttccatc aagatccaaa ccatagattc cttcttccat tgatgccaat aggcgctgtt
     1921 ggaacagtgc aagtttattc ttttcatctt ttgtgacctg acgttcaaga gctacggtaa
     1981 tacgatttgc aaccatcccc aaacttttga gcgtgaactc cgtcaacggg tgccgagcaa
     2041 ataaccccat cactcccagc acctcctgat tgcttaccaa tgggtagcca gcgaaagcca
     2101 tcatcccttc ttgcttggcc cactcctggt cggggacgcg tggatcaccg atgaccatat
     2161 tggtcagtat gggtttgcgc tcagcggcga tgaggccgat tttgaatttc cccattggca
     2221 cacggctgtg cggcccgtct aaatgggtat atagcccgga gcttgcgcac aatttcaata
     2281 cccggtctga ttcatcaagg caccaaatac gggcaaatgc tgcaccaaga tgtcgtacca
     2341 gagcgtcggt acagccctga agaaggtccg ggatggtttc cttttggttg atgacggtgg
     2401 tcacttcaat atccaactcg agcaggcgat tgcgctccat cagatccaat tcggcctgtt
     2461 tacgtttcgt aatgtcgtgt gtgacagcta aatgttccac ttcgttcgtg atgggattct
     2521 tgaatggcac cgcatacgtt tctatccaaa ttctggtgcc tttgatgctc tggacctcaa
     2581 attgaagagt ccgtttcttc ccctgtatca cctcctgatg catttgctta aaggcatcca
     2641 aatgctcagg gacgacgagg tcaaagacgg aatgtcccag cacctcgctt gcatgttccg
     2701 catcgattaa atccaatccc gcagggttga tgtggagcag ggtcccatcc gcggcgaccc
     2761 gttttatgca accaggtcct gtctcgaaaa tgagatgtaa caaaatttct cgttccctgc
     2821 gctccaactc cgcctgtttc tgcgcagtaa tgtcatcgga gatccctagg agatagagcg
     2881 gatcaccgtt ggcatccaga agcggaattt ttttggtatg cagatatctg gtcccgtgtg
     2941 atttggtatg aataacttcc tcaggaacat ccagcagggt tttgcttgcc aagacttctc
     3001 gatcttttcc tgtaaagaag tcggcttccg atttggagaa aaaatcgtaa tccgtctttc
     3061 caatcaacgt ctccgtagga tagccaacga gtgcctctcc agccttattg aagcgaagat
     3121 atcgaagatt tttggcctct ttgacaaaaa tcgtataagg aatattttcc acaatggagt
     3181 tcaaaaacag ttcactattc cgcaattcga tatctgcctg tttccggatc gtgatatcct
     3241 gaacggtgcc aagcattcgc tgcggatgtc cttgctgatg ttcaacaacc tgtcccttgc
     3301 aagcgaccca ccggacctcc cctgtcggcc acacaactcg atgctcgact tcgtagggcg
     3361 catcgttctc taacgttctt cgaatggatt ctcccaacaa caaccgatct tcaaaatgaa
     3421 cacaattcat atacgcttca tttgtcccat caaatttccc tgaacccaac ccaaaaatcc
     3481 gagagacttc ctcggaccag atgaggtgat tggttgggat atgccaatcc caaatcccca
     3541 tattccctgc cttcaaggcc agattgagtc gccgttcact ttccgaaagc aacgtgtggg
     3601 tgcgttgctg ttcgacctga cgttgagctt cacttgacgt gagcgcctct tccgcctgca
     3661 cggctttcca agccagattt ttgaccgcta ccgcccgatg caccatttcg accacctcat
     3721 gccggtcata cggtttgatc aaatgggcaa aggcatgatg ttgcaaagta gcgatttttt
     3781 ccttttgatc gccatgcgca gtgagaatga tgacggggag tgtggcatcc tgactatata
     3841 accgctgcag gacgatcagg ccatcaaggt ccggcagacc taaatccaga atgaccgcgc
     3901 tgtagggatg aggaaggagt gcacgacgga gggcttcgtt tccggtctcc accgaatcca
     3961 cctgatagcc ttcgttgccc aataaatcgc ttagggccag acaaatatcc gtctcgtcat
     4021 ccacaacaag gatagttggt ggggtggtcc tcgcagcttc catatttctt cctttattgt
     4081 cctactcatt ccgttaaaaa gacgcatttt aaatggagga tttttgacgg aatcagagaa
     4141 ggtattctac cccttttaga aaaaggttca aaccacaggt caacgactag gatgctccta
     4201 cattctttct caatcatttc tccttttatt ttgaggacca acttgccgag gcaagaggca
     4261 ttctttgccc aacgcccttc tcaaggttcc caaacattcg caaaatttcc attattctat
     4321 gagtttctac ttggtttaat gacggcacga aacttgagac tgccgtatct catttggaca
     4381 atcaacaact ctgccatgca taagccgtgg acaatactta ctgaaaggct gaggacaagg
     4441 tgacctcaac gtttggtcta tgggagtttt tgagccgtga cgtgaccttc ctgggaggcc
     4501 cgcacagccc cctgctgagt tggctggggt ctggtgggat tctgcttttt tttctgtggc
     4561 acatcctcaa gatgcgaaag gagacggctt cggttcaacg ggcgttcgag cgcatccatc
     4621 caaaattaca ggctctggcc aatgagcgaa ggaagatgga tcgcgatcgc ttcacccacc
     4681 accacggaaa tgggttctca acccatgctg accgggctgc atcttcttca aaccggctgg
     4741 acagtgagga catccaaacg ctcgatgcgg gaatgcaaca ggaacccatc tttcgcagcc
     4801 cgtgggcgca atatcgaatg actctgattc tggaacaggt cccttggttc gtggacccgc
     4861 gtatattcag taccaaacct gctgcagagg tatttaccca agaaaccctc atcgccaagc
     4921 acgtaaatct tactttctat aggcaatttc cttcacttgt caccagcctc gggcttctcc
     4981 tgaccttcct cgctttgttt atcggccttg gcaagcttca tgcggatggg agtgaaattg
     5041 tagggattca gggtttaatt aacggattag ccggaaagtt cctaacgtcc atcgttggct
     5101 tgatcgtggc gaatatcttc tcgttccttg aaaaaccaat agttgctcgc ctgatgaatg
     5161 cccatcacgc atgcctcgat ctcatcgatc aactcttccc tcgaaaaact atggaacaaa
     5221 tgttggagca actcacagct aagcaaggcc aatccccgaa agatccttcc ccctcaaggg
     5281 aggaaaaccg cgaacacctt cttggatggg ggacaaacgg acttatcggt cctaccgcgg
     5341 atttaacaac ggcgattcag agcttaatga cattgcagaa ggaggatcat gctgaaattc
     5401 gacgaacggt atcggaactt ccacgcgtca tcacggacac actgcatggt cccctgaacg
     5461 aattgactgg ggccattcat gacctcacca tattgcttaa ggatatcagg cctcatcgcg
     5521 tgcaaacgga aacatcgttc gaagagcgcc cacctctctg gaaaccctct gccaactccc
     5581 gtggtccttc catgattaac ttttttgata aaatcggctc ctggtcaaaa cggccgggct
     5641 tagcccgaca ccggcggacg ggatgagttc cccgatggat caggataacg ccagttctcc
     5701 cacgacgggc ggggtcatcg atctcatgac ttcactggcc atgatcttca ttctgctctt
     5761 tgcagccttt atcactcaga cctcctccga agctcaatcg gaattacagg agcacaaaga
     5821 gaatgtgcag gcagccctcc gggaccatct cgaacgttta gggctttcgc tggattccga
     5881 tcctcaagat ccgctcacgc ttctcatcgt cgtccccgac aataaactca cctttgagtt
     5941 cggccaaagt attctttctc atgacgctgt tcagtttgta caggaggcga tgccgttcta
     6001 cgcggcagtt ttatgcggtc ccttacgcga caagatcgat tccctggcca tccaaggcca
     6061 caccgacgat cgcggcgacg atgcctttaa tttaaaatta agccaggaac gatccctggc
     6121 cgtaatggtg aaggggttag aggccataga agctcaagag ccgtc
//
LOCUS       DAORAQ010000153         6123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1885_length_6123_cov_61.988958, whole genome shotgun sequence.
ACCESSION   DAORAQ010000153 DAORAQ010000000
VERSION     DAORAQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1885_length_6123_cov_61.988958"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3000
                     /locus_tag="PKM72_11415"
     CDS             <1..3000
                     /locus_tag="PKM72_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNP61440.1"
                     /translation="PTAIADSDTDLADVDSTTFAYVTVSVSGLLDGNAEVLILDGDTF
                     ALATDAPGQDTTGGNYHVRVSTSAGTAVVTIIKQGGGTFSEVETETLIKAIRYQHQDT
                     DAPSDGDRLIEVRVNDGTTDSAAARTTINVDPGNDQPVFAGLDNTPAFTEGGATVVLD
                     GNATIADPELDAANDYNGATLTLARNGGANAEDMFAESGTLNTLTESGGLVVGGTTIG
                     TVTTNSGGTLVLTFNSNATTALVNSALQQITYANNSSNPPGSVQVDFVINDGNSGGQG
                     SGGARSDTGSITVTINGVNDPPTVDLDANDSSGATGNDYAVTFTEGGGPIGIADSDTD
                     LLDADSTTFAYVKLGVSGLLNGNAETLVLDGNIFTLATAVAGQDSTGGNYHVVIGTGA
                     GTATVTITKQGGGTFTEVETETLLKAIQYQHTDTNAPTDGDRLIDVYVNDGTEDSVAA
                     RTTINVNPVNDPPVAVGDGFIVNEGATTRLNLSNNDGDPDDGLDLTSITIVSAPSNGT
                     ITSVNTDGTVDYTHDGSETLADSFTYTIRDFSGLTSNTVTVDLTVTPVNDPPTAVADS
                     FTVNEGSTNTLNLSINDTDPDNGLDLTSITIVSGPANGAITSINSDGTVTYTHNGSET
                     LADSFTYTIRDLSGTTSNIVTVDLTVTPINDAPVITSNGGGTTANISVTTGTRAVTNV
                     HATDADGNPLTYSIIGGADAALFTMDPVTGVLTFITAPDFQAPGDIGADNIYDVIVQV
                     SDGTSVDTQAIAVAVTQTNVPPQIFIPTPQDPAPSSDPPPRDSGDEPSEDQAPDNGGS
                     FDPGSPGNIPDGGQGSTITGAKNSVTENTLGRHELPAKQEDGERTGFAGMVSDMWSVL
                     KKPLDITAFKDEIRSLLHRSGFLQDLDRVRDDVQEVADTEQAYLASSIAVSTGLSIGY
                     VVWLLRSGVLLTALLSSVPAWQFVNPLLVLDAAAKKKRQSGQKGVEGDSVESLFEKPT
                     ASSEIAEEKTGAPAKAPRARWFNWNKG"
     gene            2997..5525
                     /locus_tag="PKM72_11420"
     CDS             2997..5525
                     /locus_tag="PKM72_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP61441.1"
                     /translation="MNILSTKSRLAFGQVSLLISVLLTASLFGLMPDRTGAIRQGRAA
                     LAESIAANSTILITQEDVERLEGILQLIVDRNPDLLSAGLRTESGDWLVTIGDHENHW
                     RDNQSDYSSNTQVTVAIWEGEDKWGQVELRFTPVSDDGWLAFITGQQTQLLLFVALMC
                     FIGFYLYLGKMLKQLDPSQAVPERVRSALDTMAEGLLVLDNKGQIMLANRAFATLLTS
                     TPEALLGRKVGDFPWSDIEGHPLAPGTHPWHIALNSGEAQKNERVRLTLPDGTRLTFM
                     INCSPILGSGAKNVGVLVSFDDVTQLEEAEIELLKSKEEAVAANQAKSAFLANMSHEI
                     RTPMNAILGFTELIKRGYGRDSVETRKHLEIIHSSGKHLLDLINDILDLSKVESGRLE
                     IERVRFNPYLVIMDVVRVLGVKAHEKGIELELKIPDDVPETIIGDPGRIRQIITNLVG
                     NAVKFTERGAVTVTAHAKQVAGELIQFHIDVADTGIGMNVEATQRIFEDFVQADASVT
                     RKFGGTGLGLAISRKFARALGGDITVDSQPGVGSVFKITLDAGSAVEVAWVTPEQALT
                     IDESGMMQEHTNWVFPSARILVVDDGPENREFVKVVLEGYGLSIDEAGNGLIGVEMAM
                     ASEYDIILMDMQMPEMDGLTATRTLRDRGLQIPIISLTANAMKGFEQELFDAGFSDYL
                     TKPIDIDRFLAKLAQFLHGQPVGESSRPSVLPESSHHQASQIEDSSPIVSKLGSTNPK
                     FANVIARFVGRLSEQLQAMDAAYSNRDMEALAKLAHWLKGAGGTVGFDVLNKPAAELE
                     AAAKAADPAIIEQHLHRIHLLATRIAHGAQRTQEPVTMEMPSAS"
     gene            5770..>6123
                     /locus_tag="PKM72_11425"
     CDS             5770..>6123
                     /locus_tag="PKM72_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61442.1"
                     /translation="METEQTDQRAYPSQNNDGVLLAKPVLNELGCDETSLTQATIMMV
                     DDEETTMEIMRAYLEDAGYQNFVLVEDSSRAMQQIEEYRPDILLLDLMMPNVPGFTIL
                     QLVRDHPKLKHLPVII"
BASE COUNT         1452 a         1698 c         1692 g         1281 t
ORIGIN      
        1 cccacggcga ttgccgacag cgacaccgat ctggcagatg tggacagcac gaccttcgct
       61 tacgtgaccg tgtcggtcag cggcctgctg gatggcaatg cggaagtctt gatcctcgac
      121 ggggatacct ttgctctggc caccgatgcg ccaggccagg ataccaccgg cggcaactac
      181 catgtgcgtg tatccacttc cgccggcacg gccgtagtga cgatcattaa gcaggggggc
      241 gggacattta gtgaggtcga aacggaaacg ctcatcaagg caattcgata ccagcaccag
      301 gatacggacg caccatctga tggtgaccgg ttgatcgagg tgagggtgaa tgacggcacg
      361 acagacagtg ccgccgcccg aacgaccatt aacgtggatc ctggcaatga ccagcctgtc
      421 ttcgccggtc tggacaacac gcccgccttc actgaagggg gtgccacggt ggtgcttgac
      481 ggcaatgcca ccatcgccga ccctgaacta gatgccgcca atgattataa cggcgcgacg
      541 ctcacattgg cccgaaacgg cggcgccaat gcggaagata tgttcgctga aagcggcacg
      601 ctgaataccc tgaccgaaag cggtggcctg gtggttgggg gaacgaccat cggcaccgtc
      661 accaccaaca gcggtgggac attggtcctc actttcaaca gtaacgccac caccgccctg
      721 gtcaacagtg ccctgcaaca gatcacgtat gccaataaca gcagcaaccc accgggtagc
      781 gtgcaggttg attttgtgat taacgacggc aactcgggcg gacaaggcag cgggggcgca
      841 cggagcgata caggatccat taccgtcacc atcaatgggg tcaatgaccc gccgaccgtg
      901 gacctggatg ccaacgacag ttcaggtgcc accggcaacg attatgccgt tacgttcact
      961 gaaggcggcg ggccgattgg catcgccgat agtgacacgg atctgttgga tgcggacagc
     1021 accaccttcg cctatgtgaa actgggcgtg agcggactgc tgaacggcaa tgccgagacg
     1081 ctcgtgctcg acggcaatat ctttactctg gcgaccgccg tggccggaca ggacagcacc
     1141 ggcgggaact atcatgtcgt gattggcacc ggagccggaa cagccactgt cacgattacc
     1201 aaacaaggcg gtggcacgtt tacagaggtc gagacagaaa cgttgctcaa ggcaattcag
     1261 taccagcaca cggatacgaa cgctcccacc gacggagacc gattgatcga cgtgtatgtc
     1321 aacgacggca cggaagacag tgtcgctgca cgcacgacga tcaacgtgaa tcccgtgaac
     1381 gaccccccgg tagccgtggg ggacggtttt attgtcaatg aaggggcaac cacccggctg
     1441 aacctttcca acaatgatgg cgaccctgat gacggactgg atctgaccag catcaccatt
     1501 gtctccgccc ccagcaatgg cactatcacc agcgtaaata cggatggaac ggtagattat
     1561 acccatgatg gctccgaaac ccttgccgac agcttcacgt atacgatccg ggatttctcc
     1621 ggccttacct cgaatacggt cacggtggat ttgaccgtga caccggtcaa cgatccccct
     1681 accgcagtag ccgacagctt caccgtcaac gagggatcca caaatacgct gaacctttcc
     1741 atcaatgata ccgatccgga taacggattg gatctgacca gcatcaccat tgtctccggt
     1801 cccgctaatg gcgccatcac gagcattaat tccgatggga cggtgactta cacccataat
     1861 ggctcagaaa cccttgccga cagtttcacc tatacgatcc gcgatctctc cggtaccacc
     1921 tcgaatattg tcacggtgga tttaaccgtg acaccaatca acgatgcgcc ggtcatcacc
     1981 tcgaacgggg gcggtaccac cgccaatatt tccgttacca ccggcaccag ggcagtgacg
     2041 aatgtgcatg ccacagatgc ggatggaaat cccctgacct acagcattat cggcggagcc
     2101 gatgcggcat tatttaccat ggatcccgtg accggtgtcc tcacattcat cacagctccg
     2161 gatttccagg cgcctggcga tatcggagcc gataacatct atgacgtgat cgtccaagtc
     2221 tccgacggca cgtccgtcga tacacaggcc attgccgtcg cggttaccca aaccaatgtt
     2281 ccgccacaaa ttttcatacc cacgccacag gaccctgcgc cttcatcaga tcccccgccg
     2341 agagattccg gcgacgagcc ttcggaggac caggcgcctg acaatggtgg cagttttgat
     2401 cccggctcgc cagggaatat ccctgatggc ggccagggtt ccacgattac gggtgccaaa
     2461 aattcggtta ccgaaaatac gctgggacga cacgaattgc cggctaagca agaggatgga
     2521 gagcgcacag gcttcgcggg aatggtgagt gacatgtgga gtgtgctcaa aaagcccctt
     2581 gatatcacag ccttcaagga tgaaatccgg tcattgctgc acagatcagg attccttcag
     2641 gacctcgatc gggttcgcga cgatgttcag gaggtcgccg acaccgaaca agcctatttg
     2701 gcatccagca ttgccgtctc caccggcttg tcgattggct atgttgtctg gctcctacgc
     2761 agtggggtgc tcttgacggc cctgctttcg tcggtgccgg cctggcagtt tgtgaacccc
     2821 ctgttggtgt tagatgcggc ggcgaagaag aagcgccaaa gtgggcaaaa aggcgtggaa
     2881 ggcgattcgg tggagtcact gtttgagaaa cccaccgcgt cttccgaaat agctgaggag
     2941 aaaaccgggg ctcccgctaa agcccctcga gcccgttggt tcaattggaa taagggatga
     3001 acattctttc gacaaagtcc cgtctggcct tcggccaggt cagtctcctt atcagtgtgt
     3061 tgctaacagc cagcttgttt ggtttgatgc cggaccgaac cggcgcgatt cgccagggac
     3121 gtgcggccct tgccgagtcc atcgccgcca atagcacaat tctcattacc caggaggatg
     3181 ttgagcgcct ggagggaatt ttacagttga tcgtggaccg aaacccggac ctgttatctg
     3241 ccggattgcg aactgaatcg ggtgattggc ttgtcacaat aggtgaccat gagaaccatt
     3301 ggcgggacaa ccagtcggac tattcttcaa atacccaggt aacggtcgct atctgggaag
     3361 gggaagacaa atggggccaa gtcgaactgc gattcacacc ggttagtgac gacggctggc
     3421 tcgcgtttat caccggccaa cagacacaac tcctgctatt tgtggcccta atgtgtttca
     3481 tcggctttta cctgtatttg ggaaaaatgt tgaaacaact cgatccctca caggctgttc
     3541 ctgagcgtgt gcgatccgct ctggatacga tggcagaagg gctcctggtg ctcgacaaca
     3601 aagggcaaat catgctggcc aatcgggcct tcgctacctt gttgacctct acccctgagg
     3661 cgctcttggg tcgcaaagtc ggagattttc cctggtctga tattgaaggc catccgttgg
     3721 caccggggac acacccctgg catatcgcat tgaattcggg agaggctcaa aagaatgagc
     3781 gcgtccgatt gactctaccc gacggcacac ggctgacgtt tatgattaac tgctccccta
     3841 tccttggcag cggagccaaa aatgtcgggg ttttggtcag ctttgacgac gtcacgcaat
     3901 tggaagaagc ggaaatcgag ttactgaagt ccaaagaaga ggccgttgcg gccaatcagg
     3961 ccaagagcgc tttcctggcc aatatgagtc atgagatccg cacaccgatg aatgccatac
     4021 tgggtttcac agaactgatt aaacgtggtt acggacgcga ctcggttgaa acccgtaaac
     4081 atcttgagat catccattcc agtgggaaac atttgcttga cctcattaac gatattctgg
     4141 atctctcgaa ggtcgagtcg gggcgtctgg agatcgaacg ggtacgattc aatccctatc
     4201 tcgtgatcat ggatgtcgtc agggttctgg gtgtgaaggc tcatgagaag ggcatcgaac
     4261 tcgaacttaa gataccggat gacgtcccgg agaccataat cggcgatccg ggccggattc
     4321 gccagattat caccaacctc gtggggaacg cggtgaaatt cactgagcgc ggcgcggtca
     4381 cggtgaccgc gcacgccaag caggtcgccg gagaactgat ccaatttcat atcgatgtcg
     4441 cagataccgg gattggaatg aatgtggaag ccactcagcg aatttttgaa gattttgtcc
     4501 aggccgatgc gtcggtgacc cgaaagtttg gaggaaccgg cctgggatta gctatcagcc
     4561 gcaagttcgc acgcgccctg ggtggggata ttacggtgga cagtcagccg ggagtcggca
     4621 gtgtattcaa gatcacactg gatgccggca gtgcggtgga ggtggcctgg gtcacacccg
     4681 agcaggcctt aacgatcgac gaatcgggca tgatgcagga acacaccaac tgggtctttc
     4741 catcggccag gatcctggtc gtggacgatg ggccggaaaa ccgcgaattc gtgaaggtgg
     4801 tcctggaggg ttatggtctg agcatcgacg aagcgggaaa tggtctgata ggagtggaaa
     4861 tggctatggc aagcgagtat gacatcattc tcatggacat gcagatgcct gagatggacg
     4921 ggcttactgc cactcgcacg ttacgcgaca gaggcctgca gatccccatc atttcgctca
     4981 ctgccaacgc catgaaaggc ttcgagcagg agttatttga tgccggattt tccgactatc
     5041 tcaccaaacc tatcgacatc gaccggttcc tcgccaaatt ggctcaattc ctccatggtc
     5101 aaccagtcgg tgaatcatcc agaccatccg tcctcccgga gtcctcgcac catcaagcct
     5161 cacagattga ggactcctca cccatcgttt ccaagttagg gtcaaccaac ccgaaattcg
     5221 ccaatgtgat tgcccgtttt gtgggtcgcc tgagtgaaca actgcaggca atggatgccg
     5281 cctattccaa tcgggatatg gaggccttgg ccaagctggc tcattggctg aagggtgcgg
     5341 gtggcactgt ggggtttgat gtgttgaaca agccggctgc tgaactggaa gccgcagcca
     5401 aggctgccga tccggctatc atcgaacagc accttcaccg cattcacctt ctggcgacac
     5461 gaatagctca tggagctcaa aggacccaag agcccgtgac aatggaaatg ccatctgcat
     5521 catagcctgc agggtaaaaa ggccccggca gacctttcca acctcggcgg cggccccgtc
     5581 agaccttcca gctcaataac tgcatgtagt ctcagggcgt ctctatttag tcggccatca
     5641 atttttcggg tttttcgctt tttatacttt attttccctt tcgaaagacc gaaaagtttg
     5701 cacaggaaac gaacaccctt gacctgaagc cggagatacc cgcctttttt aactttgagt
     5761 gatcgcgaca tggaaacaga gcagacagac cagagagcat acccatcaca aaataacgac
     5821 ggggtgctcc tggcgaaacc cgtccttaat gaattgggat gcgatgaaac ctcgctgact
     5881 caggccacga tcatgatggt cgacgacgag gaaacgacca tggagatcat gcgggcctat
     5941 ctggaggatg ctggctatca gaactttgtg cttgtggagg attcctctcg ggccatgcaa
     6001 cagatcgaag agtatcgacc ggatattctg ttacttgatc tgatgatgcc gaacgtcccc
     6061 ggattcacaa tcctgcagct tgtgagggac catcccaaac tgaaacatct tcctgtcatt
     6121 atc
//
LOCUS       DAORAQ010000154         6028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1920_length_6028_cov_65.493052, whole genome shotgun sequence.
ACCESSION   DAORAQ010000154 DAORAQ010000000
VERSION     DAORAQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_6028_cov_65.493052"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PKM72_11430"
     CDS             complement(<1..853)
                     /locus_tag="PKM72_11430"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNP61443.1"
                     /translation="MKIDRRFTKTGESPYQAIPFSTRSSEIRNPDGSAVFHQDNILAP
                     EHWSQLAVDILAQKYFRKAGVPQFDDQGHPLLNEKGGPLLGGERDARQVFHRLAGCWT
                     EWGKTHHYFDTPEDAETFKDELSYMLAWQMAAPNSPQWFNTGLHFAYGLSGPSQGHFY
                     VDPNTHRVKQARNAYERPQVHACFIQSISDDLVNEGGIMDLWVREARLFKYGSGTGTN
                     FSKLRADGESLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDIEE
                     FIDWKVIE"
     gene            1608..1898
                     /locus_tag="PKM72_11435"
     CDS             1608..1898
                     /locus_tag="PKM72_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61444.1"
                     /translation="MYVWSKPLVLGLVLALVLLEGMFFLMIGEGGESQLHVRTTQWIT
                     ANYVLLMIWIFVWMYDQARVRGKNVWLWILPFALFPLPTLAFFIFRLQRRIA"
     gene            complement(1905..3284)
                     /locus_tag="PKM72_11440"
     CDS             complement(1905..3284)
                     /locus_tag="PKM72_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNP61445.1"
                     /translation="MSDGFGKLLIARCVGFRLLPGQIVASAALGLILFGACLLMLPIA
                     THPGVHISFLDALFTATSAVCVTGLIVMDTPQDFTLFGQGVILGLIQIGGLGYALMAT
                     MILLILGRRLGLRDRMMLSESMNTMDLQGLVRFVKLVAIITFGLESLGAVLLTLRFAV
                     DFPWWTAAYYGIFHAISAFNNAGFALFSDSFKTFQTDWTINGIITILVIFGSIGFFVF
                     EDILGNIRGQRFRLQTHTKLVLVTTALLIVGGTIGITILEWNNPSTFQSASIGKKLTI
                     SYFHSVSRTAGFTSMDIIDMRDATLYFLLLLMAIGGSPGSMAGGLKTTTAAIVFLTIL
                     SMLRRDKDVEIFNRRIPQDLITRALCFTILAFVMITGMTLLLDSTESQPFLFLMFEVT
                     SAMGIVGMSLGNGEALSLSALFTDFGKVMIMLSMLLGRFGPLMIGLFAIKTIVSKPYR
                     YAKARVIIG"
     gene            3576..4967
                     /gene="mtaB"
                     /locus_tag="PKM72_11445"
     CDS             3576..4967
                     /gene="mtaB"
                     /locus_tag="PKM72_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNP61446.1"
                     /translation="MPMNSEDFQQPQPRVTFYTLGCRLNQAETAVLKDGFQQSGFLPV
                     EFGQETDVFVINTCTVTEGAEVDCRRIVRQVLRHSPHAFVAVTGCYAQTGLEVLRRMA
                     DIDLIVGNQFKMQLTEIIPPFSQLKKQPVPLVHHGRIDPENFLIEGVGAYTTTRANVK
                     IQDGCQFMCSFCLIPFARGRERSRFLEDAVREAEELVERGHRELVLTGVNIGQYRDGA
                     ADLLVLLHRFEAIKGLERIRISSIEPTTVPESLLDYMSSSTKLCRYLHVPLQSGDDRI
                     LEAMNRRYSVREYSEAMESALKRIPDLCFGTDVLVGFPGEGSREFSNTEAVIRDLPFA
                     YLHVFSYSPRPGTASTKLPHAVSPITTKERSRRLRELSDHKRMAFQQRFIGRRVSVLF
                     EAAEAEGYWPGLTDNYIRVAVRSPHPLRNTIQPVVVTGIMSGTTLGLLEPSSEFSPTA
                     KDLYSVSSLVSVS"
     gene            4981..>6028
                     /gene="miaB"
                     /locus_tag="PKM72_11450"
     CDS             4981..>6028
                     /gene="miaB"
                     /locus_tag="PKM72_11450"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNP61447.1"
                     /translation="MSGPSLLIPTSLKARTHLPDKGRYQVYMETFGCQMNEYDSEIVR
                     SLLKTRGFQFTPYDDEADVILFNTCAIRENAHNRVYGHLARYTNKKQERGLVIGVLGC
                     MAQNLKQELMDTRSFIDVLVGPDGYRRLPDLLMDALEHRKRGLAVDLSEYETYAHIVP
                     DRPVGINGWIAVMRGCDNFCSFCVVPYTRGRERSRDPEGIIQEAHQLVSQGVRQITLL
                     GQNVNSYRSGEWDFARLLIAVGDVPGIQRVRFMSPHPKDFPPALLEAVAEHPAICKTI
                     HLPLQSANDRILERMGRGYTQREYRKLVEAIRKKGPLVLTTDLICGFCGETDEEFQET
                     YRLIQEVGYQAAFVF"
BASE COUNT         1528 a         1346 c         1628 g         1526 t
ORIGIN      
        1 cttctatgac tttccagtca ataaattctt caatgtcggg atgatccaaa tccagacaca
       61 ccattttcgc ggcgcggcga gtggtgcctc ccgatttaat ggcaccggcg gcccgatcgc
      121 cgattttgag aaaggacatg agtcctgacg agcgaccgcc tcctgataag gattctccat
      181 cggcccgtaa cttggagaaa ttggtaccgg ttccagaccc gtatttgaac aggcgagctt
      241 cccgaaccca cagatccata atcccgcctt cattgaccaa atcgtcagaa atcgattgga
      301 tgaagcaggc atggacttga gggcgctcat acgcgttgcg ggcctgtttg acccggtggg
      361 tattcgggtc aacatagaaa tgtccttgtg aaggtccaga caggccgtag gcaaagtgga
      421 gtccggtgtt aaaccattgg ggagagttgg gcgcggccat ttgccaggcc aacatgtaac
      481 tcagttcgtc tttaaaggtt tcggcatctt ccggggtatc aaagtagtga tgagttttgc
      541 cccattccgt ccaacaaccg gcgagtcgat ggaacacttg cctggcatca cgttcccctc
      601 ccagcagtgg gccgcctttc tcattgagga gtggatgtcc ctggtcgtca aattggggaa
      661 ccccggcttt acgaaaatat ttttgggcca aaatgtccac tgccagttgg gaccaatgtt
      721 ccggggccag aatattgtct tgatggaaca cggctgagcc atccgggttt cggatttctg
      781 aggatcgggt tgagaatggg atggcctgat aagggctttc acccgttttt gtgaatcgac
      841 ggtcaatttt catgtaggac tcctttggaa gagactgcaa acctgagggg taattcttcg
      901 gtcatttttt ccgcctcatc tggcatccct gccttccagg agagcacatt ggaattgtgc
      961 ctctctactg cctggtattc tctcacggat cctggagttc atacaacaag cagtgcaacc
     1021 agaagccatc aatgcagcgt atcggcgaat gactggcctc tagcaggaaa gaattggcgt
     1081 atgccaaatg cctgcacaca tcagaaaaat aggaaaaaat aaaaccggaa gaaataattg
     1141 gtataaacca atgccccaca atctatagcg gatagaataa aatgtcaata ctaaatgttg
     1201 tgactggtgc ctacttgatg gggatttctg tgcagaacat gtgagttcaa aaaacctaat
     1261 atttcgtgcg gagttccgaa tgaattttca tggttatcaa cagcctgaaa ttctagccca
     1321 acaagttatg cacacgaaga ttattcaaga cgaaaaaaat taaaaaaaat ttcagatggc
     1381 cttttatggt tctcacgacc attcgtaaaa catgaataat caggaaagtg atagaggaaa
     1441 aaatgtgttc agattgaaag cagtcacgac gggatcggca gtaagtctct gtgcgaacat
     1501 tttgatttgc tgcggtatcc tatgtcacct tatgggtttg tggcaggtgg atcagatatg
     1561 gtccatggga gattaggagg cctatttttt gaagaaggag ccgaatcatg tatgtgtgga
     1621 gtaaaccctt ggttctcggg ttggtgttgg cgttggtctt gttggaggga atgtttttcc
     1681 tcatgatcgg ggagggaggg gaaagccagt tgcatgttcg aaccactcag tggattacgg
     1741 ccaactatgt cttattaatg atttggatct ttgtctggat gtatgatcaa gcaagggtga
     1801 ggggaaaaaa tgtgtggttg tggatcttgc cctttgcttt gttcccactg ccgacactgg
     1861 ccttttttat tttcaggctt cagcggcgga ttgcctgagc agatttaccc gataatcact
     1921 cgggccttag catatcgata gggcttactg acgatggtct tgatggcaaa taatccgatc
     1981 atcaatgggc cgaaccggcc caagagcatg gatagcataa tcatgacttt cccaaaatcg
     2041 gtaaagagtg ccgataagct gagtgcttcg ccgtttccta aagacatgcc cacaatgccc
     2101 atggccgagg tgacctcaaa catcaggaat aaaaacggtt gcgactcggt agaatccagc
     2161 agaagggtca tgccggtaat catgacaaaa gcaaggatgg tgaagcaaag agcccgagta
     2221 atgagatcct gggggattcg ccggttaaag atttcgacgt ctttatctcg acgcagcatg
     2281 cttaagatgg tcagaaagac gatggcggca gtggttgttt tgaggcctcc tgccatactg
     2341 ccgggtgatc ctccgatggc catcagtaat aacagaaaat agagggtggc atcccgcatg
     2401 tcgatgatgt ccatcgatgt aaatcctgca gtacgggaaa ctgagtgaaa gtaggaaatc
     2461 gtcagctttt ttccgatgga ggcggattgg aatgtggagg gattgttcca ctccaatatg
     2521 gtaatgccta ttgttccacc cacgataagc agggcggtgg ttaccaatac caatttggtg
     2581 tgtgtctgta aacgaaaacg ttgcccacga atattgccga gaatatcttc aaagacaaaa
     2641 aacccgatgc ttccaaagat caccaaaatg gtgatgattc cattgatggt ccagtctgtt
     2701 tgaaacgttt taaagctatc agaaaaaagg gcaaaccctg cattgttaaa tgccgaaatg
     2761 gcatggaata ttccgtaata ggcggctgtc caccacggaa agtcgacggc aaagcgaagc
     2821 gtgagcaaca ccgcacctag gctctccaat ccaaaggtaa tgatggctac cagtttcacg
     2881 aaccggacta atccctgtaa atccatggta ttcatcgatt cgctgagcat catcctgtct
     2941 cgtaacccaa ggcgacgccc caatatgagt aaaatcattg tggccatcaa cgcgtaccct
     3001 aatcctccaa tctgaatcaa cccgagaatt actccttgcc cgaacagggt gaaatcctgt
     3061 ggggtatcca tgacgatgag acccgtgacg cagacggcgg aggttgcggt aaagagcgca
     3121 tcaaggaagg agatgtgaac tcctggatga gtggcgatag gaagcatgag caggcaggca
     3181 ccgaagagaa tgagtcctag cgcagcagat gccacaatct ggccaggtag taaccgaaag
     3241 ccgacacaac gggctatcag gagttttccg aaaccgtcac tcattgggta agggaagggg
     3301 gttcgtttga atgtgaatca ggatgggcgg ataggggcat ctggttcgaa ccatcaggaa
     3361 tgccaggctt aattcatgtc caagcgcagc ccttttgtta gaaacaatag gaatgaggtg
     3421 ggagacaaaa ttgaaagacg atagatgact ctcgggtaat ccccgactgg gccagactgc
     3481 atgatgataa tcgaaaaata ctagcatgtt cgcccggcaa tggaaacctg ggttcctgtt
     3541 gtcttgttgt gcggaaaggc cttctttata atggcatgcc tatgaattct gaggatttcc
     3601 aacaacccca accacgggtg acgttctata cgttagggtg tcgactcaat caggctgaaa
     3661 cggctgtcct taaggacggg tttcagcaaa gcgggtttct cccggttgaa tttggtcagg
     3721 aaacggatgt ttttgtgatc aatacctgca ccgtcacgga aggggccgag gtcgattgcc
     3781 gacgaatcgt tcgccaggtc ctccgtcatt cgccccacgc ctttgtggcg gtcaccgggt
     3841 gttatgctca gaccggactt gaagtgctcc gccgcatggc cgatattgac ctcattgtcg
     3901 gcaatcaatt caaaatgcag ctgactgaga tcattccacc attttctcag cttaaaaaac
     3961 agcctgtgcc actggtgcat catgggcgga ttgatcctga gaattttttg atagaaggcg
     4021 taggagccta taccacgact cgcgcaaatg tgaaaatcca ggacgggtgt caatttatgt
     4081 gttcgttttg cctgattccc tttgccagag gccgggagcg gagtcggttt ctggaggatg
     4141 cggttcgtga ggccgaggaa ttggtggaaa gagggcatcg tgagttagtg ctgacgggag
     4201 tgaacatcgg acaatatcgg gatggagctg ccgacctctt ggtgctcctt catcgatttg
     4261 aggccattaa aggcctggag cggatccgta tttcctcaat tgaacccacg acagttcctg
     4321 agagtcttct ggactatatg agcagttcca ccaagctgtg ccggtatttg catgtgccct
     4381 tacagagcgg agatgatcgt atactggagg ccatgaatcg gcgatattcg gtgcgagagt
     4441 attcggaagc aatggaatct gctctgaagc ggattccaga tctgtgcttt ggaacggatg
     4501 tgctggtggg gtttcctggg gagggctcac gagaattttc gaatacggaa gcggtgatcc
     4561 gggatcttcc ttttgcgtat ttgcatgtgt ttagctactc gcccaggcca gggaccgcct
     4621 cgaccaagtt gccacatgcc gtgtctccaa taacgacaaa agagcggagt cggagattgc
     4681 gggaattatc cgatcacaag cgaatggctt tccaacagcg gtttataggg cgacgagtct
     4741 cggtgttatt tgaagcagcc gaagccgagg gctattggcc ggggctgacg gataattata
     4801 ttcgagtggc cgttcgttct ccacatccgc tacgtaatac cattcaaccg gttgtcgtaa
     4861 ccggcatcat gtcgggtacg acgttgggat tgctggagcc atcctctgag ttctctccca
     4921 cggcaaagga tctgtattct gtttcttcac tcgtgagcgt ttcatagaaa ggcgatggtt
     4981 gtgagcggac cctctttact gatccctacc agcttgaagg ctcggaccca tttgcctgat
     5041 aaagggcgct atcaggtgta tatggagacc ttcgggtgtc agatgaatga atatgattcg
     5101 gaaatcgttc gttctctgtt aaaaacccgg ggatttcaat ttactccgta tgatgatgaa
     5161 gccgatgtta ttttgtttaa cacctgtgcc attcgagaga atgcccataa tcgcgtgtat
     5221 ggccatttgg cccggtatac caataaaaaa caggaacgag gcttagttat cggggtatta
     5281 ggctgtatgg ctcagaatct gaaacaagag ctgatggata cgcgctcatt cattgatgtg
     5341 ctcgtcgggc cagatggata ccggcgactg cccgatcttc tcatggatgc gttggagcat
     5401 cgcaaacgag ggttggccgt tgatctgtct gaatacgaaa cgtatgccca tatcgttccg
     5461 gatcggccgg ttggaattaa tgggtggatt gcggtcatgc ggggctgtga taatttttgc
     5521 tctttttgcg tggtgccgta tacgcgagga cgagaacgtt cccgagatcc ggaggggatt
     5581 attcaggaag cccatcaatt ggtcagtcag ggagttcggc aaatcacctt attgggacaa
     5641 aatgtcaatt cctaccggtc gggagagtgg gattttgccc gattgttaat agcggttggg
     5701 gatgttcctg ggattcagcg tgtgcggttt atgtctcccc atcccaaaga ttttccgcca
     5761 gccctgcttg aagccgttgc ggaacatccc gccatctgta aaacaatcca tttgcccctg
     5821 caatcggcca atgatcgaat tctggaacgc atgggtcggg gatacactca aagagagtac
     5881 cgtaagctgg ttgaggccat acgaaagaaa ggccctcttg ttttgaccac cgatctcatt
     5941 tgtggatttt gtggagaaac cgatgaggag tttcaggaga cctatcgtct tatccaagag
     6001 gtgggatatc aggcggcatt cgtattta
//
LOCUS       DAORAQ010000155         5919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1958_length_5919_cov_71.181787, whole genome shotgun sequence.
ACCESSION   DAORAQ010000155 DAORAQ010000000
VERSION     DAORAQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_5919_cov_71.181787"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..569
                     /locus_tag="PKM72_11455"
     CDS             129..569
                     /locus_tag="PKM72_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNP61448.1"
                     /translation="MATTTIAKFMEHDHDRLDEILNEFHRIKKQDAQKARALFQELFN
                     GLHRHITWEEEILFPVFEAQADMVREHGPTEVMRNEHRQIKYFLEQIQTKFAEGDLRT
                     EEGEEGLLQVLTAHNVKEEKILYPWIDNCVSDEERASLLARMGN"
     gene            complement(618..2300)
                     /locus_tag="PKM72_11460"
     CDS             complement(618..2300)
                     /locus_tag="PKM72_11460"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNP61449.1"
                     /translation="MSHSSINLDVANRIPLQPIAEVAWEKLQLDASQIELFGRYKAKI
                     PLASLPPFPSKPESKLILVTAMSPTTAGEGKTTTLIGLVDGLNKIGERAVAAIREPSL
                     GPCFGMKGGGCGGGYAQVVPMTEINLHFTGDFHAITSAHNMLAALVDNHVHWGHKPQI
                     DPRRVTWGRVLDVNDRSLRSVVTGLGGKSNGFTREGRFDITAASEVMAILCLATDLQD
                     LQQRLGNIKVGRTVNNEMIFAKDIQAEGAMTVLLKEALNPNLVQTLEHNPALVHGGPF
                     GNIAHGCSSVVATKAALTLADYVVTEGGFGADLGAEKFFNIKCRRSGLKPACAVVVGT
                     IKALKLHGGAKEKELEQEDLDALRQGLPNLLRHLENVKKFGVPAVVAINQFTSDTKRE
                     LELVEQACADHGVKMALANHWAEGGAGALCLAETVREVIEQEPADFRVLYPDTIPLAE
                     KVRIIAREIYRAEDVEIPPAVHARFAELEKHGFGHFPICMAKTQYSFSTDPTKKGAPT
                     GFTLPVREVRLAMGAGFVVVLTGDMMTMPGLPRVPAAQSIGVDAKGDIVGLF"
     gene            2625..3938
                     /locus_tag="PKM72_11465"
     CDS             2625..3938
                     /locus_tag="PKM72_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNP61450.1"
                     /translation="MRKLFSTVFLFTWFTLCGWSFTFAQDFQVGQSVMLEATKPIGVP
                     LHRNPSPSYLKHVPSGTSATIEETAQNGQWVYLRFPNDDKAWVHKKYLKAGSPQPGPP
                     AKPENSRTAEGGEQAVWASRDQCEAVVKQGNRMAAESSSKIRLATWNIRWFPIGQPTD
                     QSEDHAEPTDLEWLICAIRWMQIDILAVQESLATPEANKAWDTIITSLNQQTGDKWRW
                     YRQPCGRPEDHHVGLLWNDTRVSLSQFESLWQFNAKARSATQACTFGLRPGQYAWVKA
                     REPEGVDFHLIGLHLKSGPTVFAVEDRHNALNGIDEAIGPLLARDRDVVILGDFNTMG
                     AGDWQSRDSELKNLRRKVAKEKPGFTDLTLHPQCSHYFRGRGGWLDHVLVPQEMQEVT
                     VTTVQVTGYCAVAGCERIRGNYPLAYRHLSDHCPVVLEIENTDKD"
     gene            complement(4024..4947)
                     /locus_tag="PKM72_11470"
     CDS             complement(4024..4947)
                     /locus_tag="PKM72_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNP61451.1"
                     /translation="MASSNKKVIYAALIGNSLIAITKFAASAMTGSSVMLAEGIHSLV
                     DTGNQGLLLYGLHRAKRPADERYPFGYGKEIYFWSFAVAIMVFALGAGISLYEGVLHI
                     LYPEPIINPMVNYIVLSLSLLFEGAAWYMAFREFSRAKGKWSYLEAVQRGKDPTIFMV
                     LFEDSAAVLGLAVAFVGIWLGQVTGIVYIDGIATCIIGLILAGIAMWLAYETKGLLIG
                     ESANKEVVQSIRDMAKQLPGVEHVNEVLTMHMGPNFILVNLSVDFRDGVTSEEVERGV
                     ATLDKDIKVKHPLVKRVFVEAEARRILKVLE"
     gene            complement(5135..>5919)
                     /locus_tag="PKM72_11475"
     CDS             complement(5135..>5919)
                     /locus_tag="PKM72_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNP61452.1"
                     /translation="PTTHIFKKPIGRLPNGLDLSNSVENEYYCLKLASAFGLPVAQAD
                     MYTFGNTQALVIERFDRRWTKDNRLLRLPQEDCCQALSVPPTRKYQSHGGPGMVQILD
                     LLRGSDNPAGDQKIVLKAQILFWMIGATDGHAKNFSIFLSPGGSFHLTPLYDVLTAQP
                     SLDAHQIERKQMKLAMSVGTSCHYRIDEILWRHFIQTGEAAGLPKNLVPEAIEEMADT
                     APKAIETIESELPVEFPAAIHTSVKNAITDRLSKLSVGKAKG"
BASE COUNT         1444 a         1675 c         1475 g         1325 t
ORIGIN      
        1 tagaagtaaa gaaggtttgc gcggcgaccg aggagtaatg ccgtggattc tcttgacgct
       61 tttatggtga tcagtccagg ataattcgag accaccgggt gcccccagcc tatatgagag
      121 gagaacctat ggccacaacc actatcgcga agtttatgga acatgaccat gaccgtctgg
      181 atgagatatt aaacgagttt cacagaatca agaagcagga tgctcaaaaa gcccgggcac
      241 tgtttcaaga attgttcaac ggactgcacc gccatatcac gtgggaggag gagattcttt
      301 ttccggtctt tgaggctcaa gcggacatgg tcagagaaca tgggcccacg gaagtcatgc
      361 gaaatgaaca tcggcagatc aagtactttc tggaacagat tcagacaaaa ttcgcagaag
      421 gagatcttcg caccgaggag ggggaagaag gcttactcca ggttctcacc gcccacaatg
      481 tgaaagagga aaaaatcctt tatccctgga ttgataattg cgtcagcgac gaagagcggg
      541 cttccctgct cgcccggatg ggaaattgat tcccgcggcc ggttcccctt ctgccaccca
      601 cgttcacgtg ctccttttca aaacaacccg acaatgtctc cctttgcgtc aaccccgatc
      661 gattgagccg ccggaacacg aggcaagccg ggcatcgtca tcatatcccc ggtaaggacc
      721 accacaaatc ctgcgcccat cgcgaggcga acctcgcgaa cgggaagcgt aaatccggtt
      781 ggagctcctt ttttggtggg gtcggtggaa aagctgtact gcgtcttcgc catgcagatg
      841 gggaaatgac caaatccgtg tttttcgagt tcggcaaaac gggcgtggac agccggcgga
      901 atctcgacat cctcagcccg ataaatctct ctggcaatga tgcgcacttt ttctgccaac
      961 ggaatcgtat caggatagag gacgcgaaag tcggcgggtt cttgttcgat cacctcacga
     1021 accgtttcgg ccaggcacag cgcaccggct ccaccctcag cccaatgatt cgccagggcc
     1081 attttgaccc catgatccgc acaagcctgt tccaccagtt ccaactcccg cttggtatca
     1141 ctcgtgaatt ggttgatggc cacgaccgcc gggacaccga attttttcac attttccaaa
     1201 tggcgcaata aattcggcaa cccctgccgg agggcatcca gatcttcctg ttcgagttct
     1261 ttttctttcg ccccgccatg aagttttaaa gccttgatcg tcccgaccac cacggcacat
     1321 gccggcttga ggccggatcg tcgacattta atattaaaaa acttctcggc tccgagatcg
     1381 gcgccaaacc ctccttcggt caccacatag tccgcgaggg tgagagccgc cttggtcgcc
     1441 acgacggaac tacacccatg ggcaatgttc ccgaacggtc cgccatgcac cagagccgga
     1501 ttatgttcca aggtctggac aaggttggga ttgagcgcct ccttgagtaa aaccgtcatg
     1561 gcaccctccg cctggatatc tttcgcaaag atcatctcgt tgttcaccgt ccgccccact
     1621 ttgatattgc ccaagcgctg ttgaagatcc tggaggtccg tcgccaaaca gagaatggcc
     1681 atgacctctg aggccgcagt gatgtcaaac cgtccttccc tcgtaaatcc gttggatttt
     1741 ccgccaagcc cggtgaccac ggatcgcagt gaccgatcat tcacatccag cacccgcccc
     1801 cacgtgaccc gacggggatc aatctggggt ttgtgccccc aatgcacatg attgtctacc
     1861 agagccgcca acatattgtg ggcagacgtg atcgcatgga aatcccccgt gaaatgcaaa
     1921 ttgatctcag tcatgggaac cacttgggca taccctcccc cacatccacc gcccttcatc
     1981 ccgaagcagg gacccagact tggctcccga attgccgcaa ccgcccgttc gccgatctta
     2041 ttcaatccat ccaccagacc gatgagcgta gtggttttgc cttctcccgc cgtcgttggg
     2101 ctcatggcgg tgaccagaat gagcttgctc tccggcttgc tcggaaatgg tgggagtgac
     2161 gctaaaggaa ttttagcctt ataccgcccg aacaattcga tttgactagc atccagttga
     2221 agtttttccc aggcgacttc ggcgatgggt tgcaatggta tgcggttggc gacgtcgaga
     2281 ttaatggaag aatgactcat caggctgact cctcaaataa acgtgatggg aatacggtct
     2341 caatatttca tgcattatgg catactcgaa gcggacatcc gaccttctcc tgccctggcg
     2401 ccttcttgca tgaaaccatg acctcgtatc gacatgctgt gttgccagcc cggtggattg
     2461 gctctggaat tcccggacaa cacaggaaat acaaccgctc aattcccggt aactgcgagg
     2521 ggatctcgca cagaggaaag aagaagggat atcagctcaa ggactatgac ggagactcca
     2581 ggtcccactt ggtattctcc ctcaagtttt gttaagtaga aggcatgcgt aaacttttca
     2641 gtacagtctt tcttttcacg tggttcaccc tgtgtggctg gtccttcacc ttcgcccagg
     2701 attttcaggt tggacaatcg gtgatgcttg aagccacgaa gcctattgga gtccctctcc
     2761 atcgcaaccc gtcaccgagc tatttgaagc atgttccgtc gggaacttcg gccaccattg
     2821 aagagaccgc acagaatgga caatgggtct accttcggtt ccctaatgat gacaaggcct
     2881 gggtccataa aaaatatctc aaagccggtt caccacagcc gggaccacca gccaaacccg
     2941 aaaattcccg gactgctgag ggcggcgaac aggccgtctg ggccagccgg gatcagtgcg
     3001 aggcggtcgt caagcaaggc aatcgtatgg ctgcggaatc ttcttccaag atccggcttg
     3061 ccacatggaa tatccgatgg tttcccatcg gccagcccac tgatcaaagt gaggatcatg
     3121 ccgagccgac tgatctcgaa tggctgatct gtgcgatccg ttggatgcaa atcgatattc
     3181 tcgcagtcca ggaaagctta gccaccccgg aggctaacaa ggcatgggac acgatcatca
     3241 cttcgctgaa tcaacagacc ggggacaaat ggcggtggta ccgccaaccg tgcggccggc
     3301 ccgaggacca tcatgtcggt ctcttatgga acgacacccg cgtatccttg tcgcaatttg
     3361 aaagcttatg gcaattcaat gcgaaagctc gatcggcaac ccaagcctgc accttcggat
     3421 tacgcccagg gcaatatgca tgggtgaaag ccagagaacc cgagggagtc gattttcatc
     3481 tgatcgggct ccacttaaaa tcaggtccca ccgtatttgc cgtggaagat cgccataacg
     3541 cgttgaacgg gattgatgaa gcgataggtc ccctcctcgc tcgcgatcgg gatgtggtca
     3601 tactcgggga cttcaatacg atgggtgccg gcgattggca atcaagggac tccgaactga
     3661 aaaacttgcg caggaaagtt gccaaggaaa aacccggttt tactgacctg accttacatc
     3721 cgcaatgttc ccactatttt cgcggacgcg gaggctggct ggatcatgtg ctggttccac
     3781 aagagatgca agaagtcacc gtcactacag tccaggtcac cggctattgc gccgtggcag
     3841 gctgtgaacg tattcgagga aactatccgc ttgcctaccg tcatctctcg gatcattgcc
     3901 ctgtggtgtt ggaaatcgag aataccgata aagattaagc ggtgagagtg aaggggcaaa
     3961 tcggcatcca cggagcacat gccccatact tgcgcaacct cacgcacaag aagccgtgat
     4021 ggattactcc aaaactttca gaatccgccg ggcctcggcc tccacaaaca cccgtttcac
     4081 aagagggtgc ttcactttaa tatccttatc caatgtggcc acgccgcgct ccacctcttc
     4141 ggacgtgacc ccgtctcgaa agtccacact cagattcacc aaaataaaat tcggccccat
     4201 gtgcatggtc aagacttcat tcacatgctc aacgcctggg agctgtttcg ccatgtcacg
     4261 aatactctga accacttcct tattggcgct ttcgccaatc aacaatcctt tcgtttcata
     4321 cgcaagccac atggcgatcc cggccagaat caacccgatg atacaggtcg cgatgccgtc
     4381 aatatagaca ataccggtga cctgtcccaa ccagataccg acaaaggcca cagccagacc
     4441 cagcaccgcc gccgaatctt caaagaggac cataaagatc gtcggatctt ttccccgttg
     4501 aaccgcttcc aaatatgacc actttccctt ggcacgggaa aattcacgga atgccatgta
     4561 ccatgcggca ccctcaaaca gaagggagag gctcaacacg atatagttga ccatggggtt
     4621 gataatcggt tcgggataca gaatgtgcaa cacaccctca tacagagaga tccccgcgcc
     4681 tagcgcaaag accataatgg ctaccgcaaa actccaaaaa tagatttcct tcccataccc
     4741 gaacgggtag cgctcatcag ccggacgttt cgcacgatgg agaccataga gcaaaagacc
     4801 ctgattgccc gtatccacca gggaatggat gccctcggcc aacatcaccg aactgcccgt
     4861 catcgccgag gccgcaaatt tggtgatcgc aataaggcta ttgccgatca acgccgcata
     4921 gataactttt ttattggatg aggccatcag acatgcaagc tattggtgaa catcttcaac
     4981 gtgagaattg ggaactgtac ccgatatgcc agataaaaat ccggtcttcg gtgaaagcga
     5041 cggatgaacc tgacatggac gtattttcca tttcccgaca aaaggtatgt acatttggga
     5101 atgcccggga gcatccatcg ttcttcacgc atgcttatcc ctttgcctta cccaccgaca
     5161 acttgctcag acgatcggtg atcgcatttt tgactgatgt gtgaatagcc gcaggaaatt
     5221 ctaccggcaa ttcgctttca attgtttcaa tcgcctttgg agcggtatca gccatttcct
     5281 cgatcgcttc ggggacaagg tttttcggaa gccctgccgc ttctccggtt tgaataaaat
     5341 gccgccagag tatttcatcg attcgataat gacagctcgt accgaccgac atggccagct
     5401 tcatctgctt gcgttcgatc tggtgcgcat caagactcgg ctgagcggtc agcacgtcat
     5461 atagcggggt caggtgaaaa ctgccaccgg gactgagaaa gatactgaaa ttcttggcat
     5521 gcccgtccgt cgcgccaatc atccagaaca ggatttgggc cttcagcaca attttctgat
     5581 caccggccgg gttatcactc cccctcagca gatcgagaat ctgcaccatt cccggccccc
     5641 cgtgactttg atatttccgt gtgggtggca ccgagagggc ctggcaacaa tcctcctgcg
     5701 gcagacgcag cagacggttg tctttcgtcc agcgccggtc gaaccgctca atgaccagtg
     5761 cttgtgtgtt gccgaacgta tacatgtcgg cttgtgcgac cggcaggccg aaagcagatg
     5821 ccagctttag gcagtaatat tcattttcga cgctgttcga aaggtcgagg ccgttcggta
     5881 accggccaat cggcttttta aagatatgag tggtcggcg
//
LOCUS       DAORAQ010000156         5870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1973_length_5870_cov_63.907825, whole genome shotgun sequence.
ACCESSION   DAORAQ010000156 DAORAQ010000000
VERSION     DAORAQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1973_length_5870_cov_63.907825"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(456..1448)
                     /locus_tag="PKM72_11480"
     CDS             complement(456..1448)
                     /locus_tag="PKM72_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNP61453.1"
                     /translation="MADFSILLILVIGLALLFDFSNGWHDSANAVATVVSTRVLSPVS
                     AVLFAGVLNVIGAFMSTAVAKMVGKGIIDPAAINDVVIISALGGAIVWNFITLRMGLP
                     SSSSHALIGAMVGAGFIHGGVEVLQGEGLMKVMEAMVSSPLLGFLVAFFLMVGLSWAF
                     FRTSRGRAMRLFRRLQLLSAGFMALSHGANDAQKAMGIITLALLSAGHIPSAEVPKWV
                     IISCALSMGLGTALGGWKIIRTLGMRIAKLDPVHGFAAETGAGVVLMVTAHIGLPVST
                     THTITSSVMGVGAVNRLSAVRWGVTRRIAYAWIFTLPGSALLAVAIYLLLAAFL"
     gene            complement(1509..2126)
                     /locus_tag="PKM72_11485"
     CDS             complement(1509..2126)
                     /locus_tag="PKM72_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNP61454.1"
                     /translation="MFSFMPRESGFFDLFEQASQNVVEAGQCLKNLMKSFNEPQKQIQ
                     HIKDLEHKGDGFTHDIVFKLNKTFITPLDREDIHELASALDDILDEIDAVAELFLVFK
                     IQRPTPTALRLSEILHESVLEVGKGIYLMRQKKWDMKECGIRVNTLENEADRVLREAI
                     SILFEEEEDPKTVMKWKEIYENFEKGTDSCEDVLNVLERIALKHG"
     gene            complement(2405..3961)
                     /locus_tag="PKM72_11490"
     CDS             complement(2405..3961)
                     /locus_tag="PKM72_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNP61455.1"
                     /translation="MQRLAVIDIGTNSIHMVLAEIGKGFSYKIVDRIKEMARLGDGTF
                     TSHRLSPEAMDRGLTVLKRFSLLATNKGFDPILPIATSAVREAKNGGDFLKLVRKELG
                     LRVRVITGEEEARLIYLGVRNSMDLSKFPAMIVDIGGGSVELMACTQKRLQFVRSLKL
                     GAIRLKDQFLKSDPPDKKMVQRVERLVSQTLKKSLSKKRFTHCQQLVATSGMAGNLAE
                     IIYLARTGRPLPQIDMATIELDEIREVEALLRTKDTPTRLKIPGLDPRRVDTLYPGVL
                     MLRLLMEHLGVKQVRLSDKAIREGVIYDFIQQHQDGLRAEQEIPHVRRRQVLLLARRY
                     QFPKVHAHHVAKLALSLFDQTQSMHHLGEREREWLEYAAFLHDIGHHIRENKHHKHTY
                     YLITHADLPGFSSEEVGIIANVARYHRRGQPNPKHAGYRLLDRDQRRVVSSLSAILRI
                     ADGLDRSHFSVVKKIHVEPGEPLCLHVSFRYDPELELWTTERRKKFFEKNFHCRIELK
                     AAAVASKNVA"
     gene            complement(4628..5425)
                     /locus_tag="PKM72_11495"
     CDS             complement(4628..5425)
                     /locus_tag="PKM72_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNP61456.1"
                     /translation="MDKGLPDNQLTQLLQTAIDAAHVAAVPIRAYFERQNLRITEKND
                     GSPVTQADQEGEALIRSHLLSNAQIGPLDILGEEEGLQGTGTRWQWIVDPIDGTRSFI
                     HGIPLFGTIIALIDSRENFPVLGVIHLPMLGLTYAAARGQGTTCQGKALRLPEDLAIE
                     QAIIGVGDFAQFSSIQREADYHLLLSLSGYVRGYTDCFGHGLVISGALGAMVDPALNP
                     WDILATQVLIEEAGGAAMLRPSKISGKVDAIFGNRSLVQHLAQELSF"
BASE COUNT         1647 a         1543 c         1253 g         1427 t
ORIGIN      
        1 catcgatccg cgccatcccc tggactttca acacaccgct caatcggcac ttctgaccta
       61 aatacatttg atacccttcc tcctcaagcc cagccgtgga aacctgcaat gcgcctgttt
      121 gataggtttc tgccataact cctccttgtg gttacaatgc catgaattga gtgggaccca
      181 gcgagacaac ctttgaacac gtcagactaa agggagcgat gcgaagggaa ccattcccaa
      241 ttggagggac catacagcca cctcccaggc gaccaagttc tttaaaccga atcggatgta
      301 agtaaatcca caaaaacgat cagatcaatg gttctacaaa atgagagtaa acagaggaga
      361 gaagaaacgg ggatatgcgg gaaccacaac agcttcctga tgcgaggcgg caacagatta
      421 ttgaaaatca cagagaatga cggaacacag gacaactaga gaaatgcggc cagcaggagg
      481 taaatggcaa cggctaagag cgccgaaccg ggaagtgtaa agatccacgc ataggcaatt
      541 cgtctggtca ctccccaacg aaccgccgac aaccgattca ccgcccccac gcccatgacc
      601 gaagaggtaa tcgtgtgagt cgtgctgaca ggtaacccaa tatgggctgt aaccatcaat
      661 accacgccgg ccccggtttc ggcggcaaac ccatggacag gatctaattt ggcgattcgc
      721 atccccagcg ttcgaatgat cttccacccc cccagggctg tccccaaacc catagaaagt
      781 gcgcaggaaa taataaccca tttgggcact tcagcagaag gaatatgtcc ggcggaaagc
      841 aaagccaagg taataatccc catcgctttt tgtgcgtcat ttgctccatg actcaacgcc
      901 ataaacccgg ctgataataa ttgaagacgg cggaataacc tcatagccct tcctcgcgaa
      961 gtccggaaga aagcccagct caatccaacc atgagaaaaa aagccacaag aaaccctaat
     1021 aaaggagaag agaccatggc ctccatgacc ttcatcaatc cttcaccctg caaaacctca
     1081 accccaccat gaataaatcc tgcgccgacc atggctccaa tcagggcatg ggaggaactg
     1141 gaaggcaacc ccatacgcaa ggtaataaaa ttccaaacaa ttgctccccc taacgccgaa
     1201 ataatgacca catcattgat tgcggcagga tcgataattc ccttccccac catcttggct
     1261 accgcggtag acataaaggc cccgatgaca ttcaggaccc cggcaaaaag aacagccgag
     1321 acagggctca ggacccgagt cgacacgaca gtagccacag cattggcact atcatgccag
     1381 ccatttgaaa aatcaaacag gagggcgagc ccgatgacca aaatgaggag gatgctgaaa
     1441 tcagccacaa atcccaacaa atgatagatt tactggtata acttaggagg ggcgaggatt
     1501 tgagtgtttt atccgtgttt cagggcaatg cgttccagga cattcagcac atcttcacag
     1561 gaatcggttc ccttctcaaa attctcataa atttctttcc atttcatcac cgttttggga
     1621 tcctcctcct cttcaaacaa tattgagatc gcttcccgaa gaacccgatc ggcttcattt
     1681 tccaaagtat tcacccggat gccacactcc ttcatatccc attttttttg tcgcattagg
     1741 taaatcccct ttccgacctc caagaccgac tcatggagaa tctccgaaag ccttaatgcc
     1801 gtaggagtgg ggcgttgaat cttaaacacc aggaacaact cggccactgc atcaatttca
     1861 tcaagaatat catcaagcgc actggcaagc tcatggatgt cttcacgatc caatggcgtg
     1921 ataaatgttt tattcaattt aaagacaatg tcgtgggtaa atccgtctcc cttgtgctcc
     1981 aagtccttaa tatgttgaat ttgtttttgt ggttcgttaa aggacttcat taaatttttc
     2041 aaacattgtc ctgcttccac cacgttttgg gacgcttgct caaataaatc aaagaatccg
     2101 ctttcccgag gcataaaact aaacataagt cttttccttc agcaaaaacc gccagacatt
     2161 caacactgca ctttagtcta ttcggaacag gggtatctac atccagattt gaagcaggat
     2221 atttcccatc ctttctctta ttgagaaaaa ttctctgcgt gtaaaccttg acaacttagg
     2281 gcaagacaga caatgccctc gaccaagata aatgtacccg attctcgtgg gaaaacgcga
     2341 tacggaagga ctcttccaca acttaatgtg ctaattctag actatttcag atacggatgc
     2401 ccaattatgc cacatttttt gatgccacag ctgcagcctt gagttcaatt cgacaatgaa
     2461 aatttttctc aaaaaacttt tttcgtcgct cggtcgtcca caattccaat tccgggtcgt
     2521 accggaaaga cacgtgcaga cacagaggtt cgccaggttc aacatgaatt ttcttaacga
     2581 ccgagaagtg actgcgatcc aaaccatctg caattcgtaa aatagcacta agactagata
     2641 caacccgtct ttgatcgcga tccaagagcc gatatcccgc atgcttggga tttggttgac
     2701 cccgtcgatg ataccgcgcc acattggcga taatgcctac ctcctcagaa gaaaatccgg
     2761 gaagatcagc atgggtgatt aaatagtacg tatgcttgtg atgtttattc tcacggatat
     2821 gatgcccgat gtcatgaaga aaagccgcat attccaacca ttcccgctcc ctttctccaa
     2881 gatgatgcat ggattgggtc tggtcgaaca agcttaatgc caacttcgca acatgatgag
     2941 catgaacttt gggaaattga tatcgccggg ctagcaataa cacttggcgg cggcgaacat
     3001 gcggaatttc ctgttccgcc cgcaaaccat cctgatgctg ttggatgaaa tcataaatca
     3061 caccttccct aatcgcttta tcactgagac gcacctgttt gaccccgaga tgctccataa
     3121 gtagacgcaa catcagaacg ccgggataga gcgtatcaac tcgtcgggga tccaggccgg
     3181 gaatcttcaa tcgtgtcggc gtatcttttg ttctaaggag tgcctcaacc tccctgattt
     3241 catcaagttc gatggtggcc atgtcaattt gtggcagggg gcgcccggtt cgagctaaat
     3301 aaattatttc agcaaggttc cccgccatac ccgatgtcgc taccagttgc tgacagtgag
     3361 tgaatcgttt cttcgataag gatttcttca gagtctggct cacgagacgt tcgacgcgct
     3421 gaaccatttt tttatcagga ggatcggact tgagaaattg atctttcaac cggatcgcac
     3481 ctaacttaag acttcgcaca aactgtaacc gcttttgcgt acatgccatc aattccacgg
     3541 atccaccgcc aatatccaca atcatggccg gaaactttga gagatccata ctattcctga
     3601 cacctaaata aatcagtcgc gcttcttcct ctccggtgat cacgcgcact ctgagcccaa
     3661 gctctttccg aacgagcttc aaaaaatctc ctccattttt tgcctctcgg accgcactgg
     3721 tggcaatcgg aagtatggga tcaaatcctt tgttcgtggc tagcagggaa aaccgtttta
     3781 aaaccgtcaa cccacggtcc atggcctccg gggacagtcg atgggacgta aaggtcccgt
     3841 cccccaatcg ggccatttcc tttatacgat cgacaatctt gtacgaaaaa cccttcccta
     3901 tttcagccag gaccatgtga atggaattag ttccgatatc aataaccgca aggcgttgca
     3961 tagggttcac ttccttctag aataggttcg gtgatcctct ttcatgacca ccataattcc
     4021 gtggtcaata aaggcatcgg atgttgtatg aaagaaatct tcaggagggg acaaaaaaat
     4081 tatcaatgac attgatacga taccccacac cccaggcagt ttcaatagtt ggactctgat
     4141 cccccaattg ctacagttta ctccggggtc ggaatatatg aggatccacg acccaaaatt
     4201 cgacagatac ctgcggtccc catagatggc cgggcattga gtccccctat cgcttgcgaa
     4261 taattattca ataaccggcc ttcaatgcat gtgagaaaga ctgattgcgt tcgaaaaacg
     4321 actcaaaggt ttgtggctca taaacacaat ccctcagacc atctcgtgac actgtcggat
     4381 aagctgctct ttctcccatg gacaagccta ggtcaggcat ggaaatgcac acgtctgctg
     4441 tccagccttc aggaaagcat atctaaccaa gttttcctac atgttatgag gatgttacat
     4501 acgggtaaat cttagctttg tggagaagga accaaaaaac tggaggcatt cggaagatgg
     4561 atgtccgata tagagatcgg aaatgctcac cggaaataaa tctcctcccg gcaaaacccc
     4621 aagaacttta aaaggagagt tcctgcgcaa gatgttgaac gagtgaacgg ttcccaaata
     4681 tggcatcaac ctttccggat atcttcgagg gacgaagcat tgccgcccct ccagcctcct
     4741 caataagcac ctgggtagcc aggatgtccc aggggttcaa agctggatcc accattgcgc
     4801 caagggctcc actgatcacg agcccatggc caaaacaatc ggtataccct cgcacatacc
     4861 cggataggct taataggaga tggtaatccg cctcccgttg tatactggaa aattgagcaa
     4921 aatcgccaac cccgataatg gcttgttcaa tggccaggtc ttcaggaagc cgcagggctt
     4981 ttccttggca ggtggtccct tgtcccctgg ccgcagcata ggtgagtccg agcattggaa
     5041 gatgaatcac tccaaggaca ggaaagtttt cccgactgtc aatcaaggcg attatggtgc
     5101 cgaataacgg gatcccatga ataaaggatc gtgtgccatc gataggatcc acgatccatt
     5161 gccatcttgt tcccgtcccc tgtaatcctt cctcttcacc gagaatatca agaggtccaa
     5221 tttgggcatt ggacagcaag tgagaccgga tcagagcttc accctcttga tcggcttggg
     5281 tgacgggcga accatcgttt ttttccgtga tacggagatt ctgcctttca aaataggctc
     5341 gaatcggaac tgccgcaacg tgggcggcat cgatagccgt ttgcaacaat tgagtcaatt
     5401 gattatcggg caagccttta tccatcagtt atgctctcct gcgatttttg aacaccgttt
     5461 ttcctcctaa tagtatactg aacttcttgt tttggtgaca caggttcttc cgaatcctgg
     5521 ttcttaatat gggggtacca gccacaatcg gacatgatta catatggtgt tcaaacctgt
     5581 tcaacatggc ttgtccgaaa tggattccca tatcccttag accaagtgcc cacaggatag
     5641 cagcccgtgt cacagagtcc gtcacccaag cacgctggca atctgatagg gccacattga
     5701 gtctgccaca tcacttttca tttaaaaaca attagttaca caataaatac cacactttct
     5761 tgtgatccat tcagcaaaaa tgagatcctt agccttgcct ttccaacatt tcctaaaaaa
     5821 tgaggaattt cctgtgttgt tgatctccac accatcccat gtagatttca
//
LOCUS       DAORAQ010000157         5869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1974_length_5869_cov_72.450808, whole genome shotgun sequence.
ACCESSION   DAORAQ010000157 DAORAQ010000000
VERSION     DAORAQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_5869_cov_72.450808"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="PKM72_11500"
     CDS             complement(<1..2360)
                     /locus_tag="PKM72_11500"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNP61457.1"
                     /translation="MRKTGKILLLILPGLFLLYTLVGFLVIPWAITTKVPPRLSEQLG
                     RPVSIKDASFNPFLFKLQVEGFDIQEQDGSFLLGFNELFIDFEPAASLGKGAYIFAEI
                     RLKLPYGLAWVRPDGSLNLAELGSSSEAQLDDNPSQEPESLPPVRIEHISIQQGMVEF
                     RDHSRPTPFVAHFVPINLTLDDFSTQQGQTNSYSLSAERSAGEKITWEGTITLEPFQS
                     EGRLVFEDYQLPRLWTYIQDLVRFQIPQGQVTVKGHYRLSTTDQGVNVLVDGGSLRIQ
                     DLQIQEKGTITPVLTIPLFEVNGVSVDLAKQDLRITSIQSRDARFTGWVGKDGVVNYH
                     TLFSPVGSESQTAPEPETSPANSWKMVVEDLALDNFTIDFEDRQPEDPVTLLLDMLHF
                     HTSDVSMAMDRPLPIDLSFQLNETGKANLQGTLQIDPLEVALDLSLTEFALKPFQPYV
                     APFVQFEVGDGALMLRGKTHYQAGHKTQPMVTFEGGMGVSKLALDDPMQTKPFLTWEA
                     LELKELNLQIEPTSVKLQEIELTNPELVLLIDEDGGMNLKRLFSPPGPVSQEETTADD
                     KPPKAETTGEPPTPVKIEAVTLTNLMARISDRSISPNVVTNIEGLTGTIKELSSDQLA
                     KADVALQGTIDKHSPFKIAGQINPLSEEAYTDVTLTFNNLDLPTVSPYSAHFVGYPIT
                     KGKLSLDLGYKVSEKTLVGANKVLIDQLTMGKKVENPDAMSLPIPLALALLKDRKGQI
                     DIDLPVRGNMNEPDFSYGGVIWNALGNLLTKVATSPFAMVGGLVGG"
     gene            2748..4136
                     /gene="ffh"
                     /locus_tag="PKM72_11505"
     CDS             2748..4136
                     /gene="ffh"
                     /locus_tag="PKM72_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNP61458.1"
                     /translation="MFTTLTEKIEKVFKQLRGQAVLTEENITDALKEVRLALLEADVN
                     FKIVKDFIENVRGKAVGQEVLKSLTPAHQVVKVVWEELRDLLGHEQSALHLSSQPPTV
                     IMMVGLQGSGKTTTTGKLANYFKTEGKRVLLVAADPRRPAAGDQLASLGTDLDIQVHR
                     GTNEGQPGAQALQTCLDGVARGREHGYDVVLLDTGGRLQIDQELMQELVDIQTGVSPQ
                     EVLFIADSMTGQEAVSVAERFNQTLGLTGVILTKVEGDARGGAVLSIRAATGKPIKFL
                     GIGEKLDALEPFYPDRMASRILGMGDVLTLIEKAQESFSQEQAVALQKKVSSNTLTLE
                     DFRDQIKQVNKLGSFDQILDMLPGGQKIKTMMGSGGAGNAQVPEKEMKRVVAIIDSMT
                     PRERRDHTILNGNRKKRVAKGSGTSVPEVNRLIKQFLDARRMMKSLVGGQMGMGKGKK
                     RGKLIRRAIHAR"
     gene            4197..4475
                     /gene="rpsP"
                     /locus_tag="PKM72_11510"
     CDS             4197..4475
                     /gene="rpsP"
                     /locus_tag="PKM72_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNP61459.1"
                     /translation="MAVHLRLTRVGRHKRPFYRVVAADARMPRDGRFLEVIGTYDPLK
                     ASDKASFKEDRVLNWLQNGAQPTDSVRGLLRQTGILKAFRESKQAPAQ"
     gene            4566..5108
                     /gene="rimM"
                     /locus_tag="PKM72_11515"
     CDS             4566..5108
                     /gene="rimM"
                     /locus_tag="PKM72_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNP61460.1"
                     /translation="MDDPEPSQSSMVTIGRILKPFGVRGEVRIESLSDVPGRFEGLET
                     VTLDLPDGRTLETLVTSVRQINQGVILGLSAFSTPETAALYRGAWIKIPENQNLPRDA
                     DTFYQFELIGLRVEDAEGQPLGTLEEVVEYPQHHVFVIRNEDREILVPASRRTIAMVD
                     VPHGVLRLTSREWWDDTYAL"
     gene            5098..5862
                     /gene="trmD"
                     /locus_tag="PKM72_11520"
     CDS             5098..5862
                     /gene="trmD"
                     /locus_tag="PKM72_11520"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNP61461.1"
                     /translation="MRCDVVTLFPELIHSVGSQSIMKRAQEKGLLTLNVHNLRDYTDN
                     PHRTADDTPYGGGGGMVMKAEPIFQAIDRITQDLDGIRIILPSPQGIRFSHGLAHNLS
                     QETRPLLFICGHYEGIDERVRTHLQVEEISLGDYVLTGGELAALVMIDAAMRLIPGVL
                     GDPHSAQKESFVESLLDFPHYTKPVETRGMSVPDILMSGNHEAIRRWRRKESLRQTLL
                     KRPDLLDHHIWSSEDRQLLEEIEHNYTSTSSRTTRP"
BASE COUNT         1522 a         1435 c         1520 g         1392 t
ORIGIN      
        1 ctgccaccca ccaatcctcc gaccatggca aaaggagatg tcgcaacttt cgtcaagaga
       61 ttccccaagg cattccagat gactcctccg tagctgaaat ccggctcgtt catattgccg
      121 cgaaccggca aatcaatatc gatctggcct ttgcggtctt tgagcaaggc taaggcaaga
      181 ggaatcggca acgacatggc atccggattc tcgacctttt tacccatcgt cagttggtca
      241 atcaacactt tattggcacc aaccagagtc ttttcagaaa ctttatatcc gagatccaac
      301 gatagcttcc ccttcgtgat gggatacccg acaaaatggg ctgaataggg agagacagta
      361 ggaagatcaa gattgttgaa cgtgagggtc acatccgtat aggcctcttc actcaaagga
      421 ttaatttgcc cggcaatctt gaatggggaa tgtttatcaa tcgtcccttg taaggcgaca
      481 tcggctttgg ctaattgatc cgaagatagc tctttaatgg tgccggtcag cccctcaatg
      541 ttcgtaacga cattggggga aatcgatcga tccgaaatac gagccatgag gttggtgagg
      601 gtgacggcct caattttgac cggagtcggt ggctccccgg tggtttctgc ttttggtggc
      661 ttatcgtctg ccgtcgtctc ttcttgagac acgggtcctg gcggggaaaa caaccgcttg
      721 aggttcatcc ctccgtcctc atcaatcaag aggaccagtt cagggttagt caactcgatt
      781 tcctggagtt tgaccgaagt cggttcgatt tgaaggttta actctttgag ttccaatgcc
      841 tcccacgtga gaaaaggttt ggtctgcatg ggatcgtcaa gagccaattt ggacactccc
      901 atccctccct caaacgtcac catcggttga gtcttgtgcc ccgcctgata gtgcgtcttt
      961 ccccgcaaca tgagcgcgcc atcaccgacc tcaaattgaa caaacggggc gacatagggt
     1021 tgaaacggct tgagggcgaa ttcggtcagc gacaaatcca gcgcaacttc caaggggtcg
     1081 atctggagag ttccttgcaa gttggccttt ccggtttcat taagctgaaa cgaaagatca
     1141 ataggaagag gccgatccat ggccatagat acatccgagg tgtgaaaatg caacatgtcc
     1201 aatagcagtg tgacagggtc ttcgggctgg cgatcctcga aatcaatcgt gaagttatcc
     1261 aaggcaagat cctcaacaac catcttccag gaattggccg gggaggtttc cggttcagga
     1321 gcggtctgac tttcagaacc cacgggtgaa aacagcgtgt gataattcac caccccatcc
     1381 ttcccgaccc atccagtgaa acgggcatcc cgagactgta tcgaagtgat gcgcaggtcc
     1441 tgtttggcca agtcaaccga gacgccgttg acctcaaaga gcggaatcgt gagtacggga
     1501 gtaatggtcc ctttctcctg aatctgtaaa tcctgaatcc tcaaacttcc cccatcgacc
     1561 agcacattga ccccctggtc tgtcgtagac aatcgataat gccctttcac ggtcacttgt
     1621 ccttgaggaa tctgaaatcg aaccagatcc tgtatgtagg tccatagcct gggaagttga
     1681 taatcttcaa aaaccaagcg gccttcggac tgaaatggtt ctagagtgat cgtcccttcc
     1741 caggtaattt tctcccctgc actgcgttcg gctgagagtg aataagaatt agtctgacct
     1801 tgttgcgtac taaaatcatc caatgtgaga ttgattggca cgaagtgcgc cacaaatggg
     1861 gtgggacgtg aatgatcccg aaattccacc atcccctgtt gaatggagat atgctcaatc
     1921 ctgaccggag gcaaggattc cggttcttgt gacgggttat cgtcaagctg ggcctctgaa
     1981 gaggagccta attcggccaa attcaaggac ccgtcgggac gaacccatgc caatccataa
     2041 ggaagcttca agcgaatttc cgcaaaaatg taggctccct ttcctaggga agcggccggt
     2101 tcgaaatcaa tgaacagttc attaaacccc agcaaaaatg atccatcctg ttcctgaata
     2161 tcaaaccctt ccacctgcag cttaaataga aagggattaa agctggcatc cttgatcgag
     2221 actggccgac ccagttgctc cgataacctg ggaggaacct tcgtggtaat cgcccaggga
     2281 atcaccagaa aacctaccag agtatagagg aggaacagtc caggaaggat aagcagaaga
     2341 atcttgccag tttttctcat gatttggctt attttttcat ggaagaaaac agaaaggaga
     2401 agattgaatc aattaaaccc aagtaaaatt tcacacgagc cattctagat gacttcaatt
     2461 ggctggacgt atcgattcat gataacgacc tgcctctttg agagctagta tgatccatag
     2521 agtctcggga ttccttcggc attccctacc cacggagccg accttgatag ggcctgcttg
     2581 cccatcgggg gagttcccac aaaagccatg aatccaaaaa ataacccttc gaaaccatct
     2641 agattttgcc cctccaagag gttgcgctca acttgacatg tctgtcgtat gttcactatt
     2701 gtcttgggtt tgcttaaaat ggttcatgtt ccattggatc ccttttcatg tttaccacgc
     2761 tgacagagaa aatcgaaaaa gttttcaagc agttacgcgg ccaggccgtt ctgactgaag
     2821 agaatattac cgacgccttg aaagaggtgc gcctggcgct gctggaagcc gacgtcaatt
     2881 tcaaaattgt taaggacttt attgagaatg ttcggggcaa ggccgtcggg caggaagtcc
     2941 tgaaaagtct cacacccgcc catcaagtgg tgaaagttgt ctgggaggaa ctgcgagacc
     3001 ttctgggtca tgaacaaagt gcccttcatc tctcttcaca accccctacc gtcattatga
     3061 tggtcggatt gcaaggatct gggaaaacca ccacgaccgg gaaactcgcc aattatttta
     3121 aaaccgaagg caagcgggtc ttattagtgg ccgccgatcc ccgtcgccct gccgcaggag
     3181 atcagctggc ctcgctgggc actgacctgg acattcaagt ccatcgggga acaaacgaag
     3241 gtcaacctgg cgcccaggcg ctacaaacct gcctggatgg cgtcgctcgt ggacgggaac
     3301 atgggtatga tgtggtgctc ttggataccg gcggtcgatt gcagatcgat caagagttga
     3361 tgcaggaatt ggtcgatatc caaacaggag tcagcccaca ggaagtcttg ttcatcgccg
     3421 actccatgac cggccaggaa gcggtttctg tagcagaacg ctttaatcaa actctcggtc
     3481 tcactggggt catcttaacg aaagtcgaag gggatgcccg gggtggagcg gtgctctcaa
     3541 tccgcgcggc gacaggcaaa cccattaaat ttctcgggat cggcgaaaag ctggatgcgc
     3601 tagagccctt ttatcccgac cggatggcct cccgtattct cgggatgggt gatgtgctga
     3661 ccttgattga gaaagcccag gagagctttt cgcaagaaca agcggtcgca ttgcaaaaaa
     3721 aggtatcgag taatactctt acgttggaag attttcgtga tcagatcaaa caggtgaata
     3781 aactcgggtc gtttgatcaa attctagaca tgctaccagg cgggcaaaaa attaaaacga
     3841 tgatgggttc cggcggagcc gggaatgcac aggtacccga aaaagaaatg aaacgggtcg
     3901 tggccattat tgattcgatg accccccgcg aaagacggga tcatacgatt ttaaatggaa
     3961 atcgaaaaaa acgtgtggct aaaggcagtg gaacatccgt tccagaggtt aatcggctca
     4021 ttaagcaatt tttggatgcc cgacgcatga tgaagtccct ggtcggaggg caaatgggga
     4081 tgggaaaagg aaaaaagcgc gggaagctga tccgccgggc tatccatgcc cggtaataaa
     4141 ttgaagtctc cctgagcttt ttcattcatt catattcacg acagaggaga ttgccagtgg
     4201 cagtacattt gcgacttaca cgcgtgggcc ggcataaacg gccattttat cgagtagtgg
     4261 cagccgatgc ccgcatgccg cgggacggtc ggttccttga ggtcattggg acctatgacc
     4321 ccctcaaggc aagcgataag gcgtcgttta aagaagatcg ggtgttgaat tggcttcaaa
     4381 atggggctca gcccactgat tccgtcaggg ggttattacg acaaaccggt atcttgaagg
     4441 cctttcggga atccaaacaa gccccagcac aatagtctcg gcatgtcagg gtaatgggag
     4501 agaaggtgcc ctgttgcatc cggcaaagag tcttttagag atgcgacagg actattggct
     4561 aaactatgga tgatcctgag ccatcacaat cttccatggt gaccattggc cgaattctca
     4621 aaccgttcgg agtccggggg gaagtgcgta tcgaatcgct ctcggatgtt cctgggcggt
     4681 ttgaaggatt ggagacggtg acactggacc ttccagatgg gaggactctg gaaaccctgg
     4741 tgacctcggt tcggcaaatc aaccaggggg tgattttagg actttcagcg ttttcgacac
     4801 cggaaaccgc agccttgtac cgaggggcct ggatcaaaat tccagaaaat cagaatcttc
     4861 ccagggacgc tgatacgttt tatcagtttg aattaattgg tctcagggtt gaggatgcag
     4921 aaggacaacc cttaggaaca ttagaagaag tggtggaata cccccaacat catgtgtttg
     4981 tgattcgaaa tgaggatcgt gaaattctgg tcccggccag tcggcgaacc attgccatgg
     5041 tagacgtacc ccatggggtt ttgcgattaa cttcacgtga atggtgggac gacacctatg
     5101 cgttgtgatg tggtgacatt gtttccggag ttgattcact ctgtgggctc acagagcatc
     5161 atgaagcgcg cccaggaaaa aggattgctc acgctcaacg tccacaatct tcgagactat
     5221 accgacaatc ctcatcggac agccgatgat actccctatg gcggcggcgg cgggatggtc
     5281 atgaaggccg aaccaatttt tcaagccatt gaccggataa cacaggacct ggatggaata
     5341 cgcatcattc ttccttcgcc acagggcatc cggttttcac atggcctcgc gcataatctc
     5401 agccaggaga ctcgcccgtt gctatttata tgcgggcatt atgaaggaat cgacgagagg
     5461 gtccggacac atttgcaggt tgaagaaatt tctctcggag attatgtgct gacaggtggt
     5521 gaattggctg ccctcgtgat gatcgatgcg gccatgcgtc tcatccctgg cgtactaggt
     5581 gatccacatt ccgctcaaaa agagtccttt gtggaatcct tactggattt cccccattac
     5641 accaaacctg ttgaaactcg agggatgtcg gttcccgaca ttttaatgtc cggcaaccat
     5701 gaagcgattc gccgttggcg caggaaagaa tcgttacgcc agacgctgct caaacgaccg
     5761 gacttattag accatcatat ctggagtagt gaagatcgtc agttattgga agaaattgaa
     5821 cacaactata cctcgacgtc cagtcgtacg acgagacctt gaaggaggg
//
LOCUS       DAORAQ010000158         5859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1977_length_5859_cov_73.002929, whole genome shotgun sequence.
ACCESSION   DAORAQ010000158 DAORAQ010000000
VERSION     DAORAQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1977_length_5859_cov_73.002929"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PKM72_11525"
     CDS             complement(<1..1322)
                     /locus_tag="PKM72_11525"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNP61462.1"
                     /translation="MSPQEWLNVLKSAYLQGFIRRGGSAVKFAVVDEAQNTKSVSMEV
                     GDLSREQGFVTIHADSCCTKVQLIDLLFKEMARQIDWDALAFEYVQKLFREHQWKIPE
                     RREECCWSSVATLNSCDESMMKREMNAWLEGALFQDFHMSREFRLAMIQLCLAQLELP
                     DSPSKEPAAIRAWLRGELKQMATLKKLLIFEKVTRSNARDLIASLSHWTRLAGKPGMV
                     LTIDISAFTGGKDVGATSLNYSPSAVMDAYEMLRQFIDGSDAIEGLLVVVVTSQAFLS
                     DQRVGLNRYEALKLRIWDDVRDKTRQNPFAPMVRLTDQTEKFTQGSLLARVGSRKEDV
                     QHQRVIESLRAGVPSPGVVKALGCPQPMVKSKFQQMLQDAQASIHKGWTTKGMLIEGG
                     FGTGKSHLLESLSHMALDSRFVCSKVVISKETPLYSPALMFRSAIESA"
     gene            complement(1403..2296)
                     /locus_tag="PKM72_11530"
     CDS             complement(1403..2296)
                     /locus_tag="PKM72_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61463.1"
                     /translation="MSADGIDNRLKACGSSSAIGVLELPDAATPSKAVIKNVNLEQAL
                     ADIEEQAGGLVKTTKQVMASFKKIKDAAKVGDLINLRKLLEEGKEVTLSLGCDFVKTQ
                     ERLNFDEAGYLEGKAFRQELLTTAQQMGVNLYEHDGYLFSYPVLLRILHKERAVAIDR
                     MRENRLRPSVLVKRLKEVQNKPLRFKPQTFLEMVFTAYSIVVAGRGKHLIGKGTVIPL
                     LELYQLLTLLPWQASEYTRQEFGRDVYLLDKSGTITTKNNHRANFHASTGVRDATKTL
                     TVIAQGGREKTYYGISFVQAG"
     gene            2515..3339
                     /locus_tag="PKM72_11535"
     CDS             2515..3339
                     /locus_tag="PKM72_11535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP61464.1"
                     /translation="MVERFIQIFSMENITPFAVITGASRGIGAEYARALATQGFDLLL
                     VARDQNRLHELSKEIQQTTSIQVWTETLDLARPHAAETLYSLAQSFRPHVSLLVNNAG
                     FGLYGLFADMSLPTIQDMLQVHIHVTTESTRLFLPDMMIRRQGAIINVASVAGFFPIP
                     YMAEYAATKAFIISFSEAVAMEARERGVTIQVCCPGYTETDFHKTAGHCPRHMASPHT
                     PRDVVQKSLKALKSKQSLVTIGWQGLATHWITPFIPKKWLMRLSARFVRPPNTLSH"
     gene            3374..3805
                     /locus_tag="PKM72_11540"
     CDS             3374..3805
                     /locus_tag="PKM72_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61465.1"
                     /translation="MPHSHFRSFSTSLSLCLGFLLTLGCSGPRPVLYPNDHLQQVGPD
                     RAEQDIEECKQLAEDYVPEHEASTVAGNTAVGAGAGGAVGAVSGALRGGVGIGAAIGA
                     ATGATIGFIRGLFQASQPTPAHQAFVNRCLAERGYDSIGWE"
     gene            complement(3924..4781)
                     /locus_tag="PKM72_11545"
     CDS             complement(3924..4781)
                     /locus_tag="PKM72_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61466.1"
                     /translation="MFKVKKRPLKKEKPPVLYNILENYTDYDPPGNVMVCGMTEPEDL
                     EMHARVLSESYDIPLEDITGVLQDGGVYLYPHEGTLVTKGAFVCRVDPLGKDPKHTWV
                     MDLEQYAAAERMRQSYGVTLEEAMERVFYRGLPQELQDRLRQKNLGIDLTKVDSGISC
                     GGNIHFIDFRKDWSPHFKRKCVMPDGRLIETSGLHDFAELHGISIEETRALFDHGGTL
                     ALKDGGGLACQIINGQPSVARFNSRQFSKAKALAKEKGLHMLDALSEVAYQDPVLMRA
                     LRRAEIPSF"
     gene            4983..5576
                     /gene="pyrE"
                     /locus_tag="PKM72_11550"
     CDS             4983..5576
                     /gene="pyrE"
                     /locus_tag="PKM72_11550"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNP61467.1"
                     /translation="MSYKLPMPKNSLIQAFHQLEAFKWNQQEGFRLASGKTSPYYVDC
                     RIVLAHPHSRHLVAQLAYHRLKSLDFTLIGGLEIGAIPLATCISDFGYRADPQREWRT
                     FVVRKQPKDHGLGKLIEGTIHHGETALVVDDVLTTGGSLVKAAESARAQGLNVTHGLV
                     VVDRSEDEGKSHLAHMGIQLLPLLTLEDLRQTSPSTR"
BASE COUNT         1495 a         1564 c         1247 g         1553 t
ORIGIN      
        1 tcagcagact caatggcaga tcgaaacatc aatgcgggac tatacagagg cgtttcctta
       61 ctgatcacga ccttgctaca tacgaatctg gaatccaagg ccatgtggct taaggattcc
      121 agtaaatgtg atttccccgt tccaaatcct ccttcaatca acatcccttt tgtagtccaa
      181 cccttatgta tgctggcctg ggcatcctgt aacatctgtt gaaatttgga tttgaccatc
      241 ggttgtggac agcctaacgc cttgacgacg cccggactcg gtacacccgc ccgcaaggat
      301 tcaatgaccc gttgatgctg tacatcttct tttcggcttc ccactcttgc caggagactg
      361 ccctgggtaa atttttcggt ctggtcggtc agtctgacca tgggcgcaaa aggattttga
      421 cgggttttat cccggacatc atcccagatt cgaagcttta aggcttcata ccgattcagc
      481 cccacccgtt gatccgagag aaaggcttgt gaggtgacaa ccacgaccaa caacccttcg
      541 attgcatcac taccatcaat gaattgtctc agcatttcat aggcatccat gaccgcagag
      601 ggcgaataat tcaaagaggt ggcgccgaca tccttcccgc ctgtaaaggc tgaaatatcg
      661 atggtcagaa ccataccggg ttttcccgcc aggcgggtcc aatgggacaa ggacgcaatg
      721 aggtcacgcg cattactgcg ggtgaccttt tcaaatatta ataacttttt gagagtagcc
      781 atttgcttca gttcgcctcg aagccaggcc ctgatcgctg ccggctcttt agacggactg
      841 tcaggtaatt ccagctgggc taaacacaac tgaatcatgg ccaatcgaaa ttcccgactc
      901 atgtgaaaat cctgaaacaa ggccccttcc aaccaggcat tcatttctcg cttcatcatg
      961 gattcatcgc aactattgag agtggcaaca gaagaccagc aacattcctc cctccgttca
     1021 ggaatcttcc attgatgttc tcgaaacagc ttttggacat attcaaatgc caaggcatcc
     1081 caatcgattt gtctggccat ttccttaaac agaagatcaa tgagttgaac tttcgtacaa
     1141 caagaatctg cgtgaatcgt gacaaagccc tgctctcgtg acaggtcccc cacctccatg
     1201 ctcacagact tggtgttctg agcctcgtcc actaccgcaa atttgacggc cgatcctccc
     1261 cgacgaataa acccttggag ataggcggat ttcagaacgt tcaaccattc ctgtggagac
     1321 ataaattttt cctcaatatc ctgttaatct agttcgtctt ctcggctcac cctagggaaa
     1381 cttaaatacc atcccgaccc gcttatcccg cctggacaaa ggaaattcca taataagttt
     1441 tctcccgtcc cccttgcgca atcacggtta acgtcttggt ggcatcccgc accccggtac
     1501 tcgcatgaaa gtttgcccga tgattgtttt tggtggtaat ggttccactt ttatccagta
     1561 aatagacgtc ccgcccaaat tcttgtctcg tgtattcact ggcctgccaa ggaaggagcg
     1621 taagaagttg atagagttcc aaaagtggaa tcacagttcc tttaccaatg agatgcttcc
     1681 ctcggcccgc caccacgatg gaataggctg taaaaaccat ctcgagaaac gtttgtggtt
     1741 taaatcgaag gggtttattt tgaacttcct ttaatcgttt gaccaagacc gaaggacgaa
     1801 gccgattttc ccgcattcgg tcaatggcga ccgcccgttc tttatgaagt atgcgcaata
     1861 agactggata ggaaaacaaa tacccatcgt gttcatagag attgacaccc atttgctgcg
     1921 cagtcgtgag aagttcctgg cgaaacgctt tgccttccag gtacccggct tcatcaaagt
     1981 tcagacgttc ctgggttttc acaaagtcac aacccaaact cagcgtcacc tctttccctt
     2041 cctctaataa ttttcgaaga ttgatcagat cccccacttt tgcggcatct ttaatttttt
     2101 taaacgaggc catcacctgc tttgtcgtct ttactagtcc tccggcttgt tcttcaatat
     2161 cagccagggc ctgctctaaa ttgacgtttt ttatgacagc ctttgatggt gtagcggcat
     2221 caggcaactc aagcacacca atcgccgagc tcgacccgca ggctttcaac ctattgtcaa
     2281 ttccgtcggc actcatatcg gttatcgagt cctctggttt gtggtgaatt caggtaatcg
     2341 gcttcaaata accgattgat cggcgcatac cggttttatt cggcacatcc tcgataattc
     2401 ttaataatat ccggcttatt tcatgatcat gctttgttta aaacccataa cgactttttt
     2461 atgctcagcg cttaacattc aatccacagt tgacctgtag gaagaaagcc ttttatggtt
     2521 gaacgattta ttcagatttt ttccatggaa aatatcacgc catttgctgt catcacagga
     2581 gcttcacgag ggattggagc cgaatatgcc agagccttag caactcaagg ttttgacctt
     2641 cttttggttg cccgggacca aaaccgtcta catgaattat ccaaggagat tcaacagacc
     2701 acttctattc aggtctggac agaaacactg gatttggcca gacctcacgc ggcggaaacc
     2761 ctttacagtt tggctcaatc atttcggcct catgtgtcat tgcttgtcaa caatgccggt
     2821 tttggcctgt atggtttatt tgcagacatg tcactcccaa ctattcaaga catgctacag
     2881 gtccatatcc atgtgaccac agaaagcaca agactcttcc tacccgatat gatgattcga
     2941 cggcagggtg caattatcaa tgtggcgtcc gtagcaggat ttttcccaat tccctatatg
     3001 gcggaatatg ccgccaccaa agcctttatc atatcttttt ctgaagccgt ggcgatggag
     3061 gccagggaaa ggggggtgac catccaggtg tgctgtcccg gctataccga aaccgatttc
     3121 cataaaacgg caggacattg tccacgccac atggcttccc ctcacactcc acgggacgtc
     3181 gtacaaaaat cattaaaggc cctaaaatcc aagcaatccc tcgtgactat tgggtggcaa
     3241 ggactggcaa cacactggat cactccattc attcctaaaa aatggctcat gcgtctaagc
     3301 gccagatttg ttcggccacc aaacaccctc tctcattaaa cacacgattt cccatattaa
     3361 aggagcacag agcatgcccc attcccattt tcggtcattt tcaacttcac tcagcctctg
     3421 tcttggcttc ctcctgacct tgggatgttc aggccctcgc ccggtcctct atcccaacga
     3481 tcatcttcaa caagttggcc ccgatcgagc tgaacaggac attgaggaat gcaaacagct
     3541 tgcagaggat tacgtcccag aacacgaggc ctcgacggtg gctggaaata ctgccgttgg
     3601 ggcgggagca gggggtgccg tcggagcagt cagtggcgcc cttcgaggag gagtgggtat
     3661 tggagcagcc ataggtgccg cgacaggtgc gaccattggc tttatccgtg gcttattcca
     3721 ggcttcgcaa cccacacccg cccatcaggc gttcgtcaac cgatgtcttg cggaacgagg
     3781 ttacgattcg attggttggg aatagatcag ggaaggaaca tgacggaaat ggcgtgaggt
     3841 cagcttgaga cgctatccct ggtggttatc accttgagaa aatccagtat caaaacttgc
     3901 tccacaggaa aatcagggaa catttagaat gaggggattt ctgcacgacg gagcgctcgc
     3961 atcaacacgg gatcttgata agccacttca cttaaggcgt ccaacatatg aaggcctttt
     4021 tccttggcga gagctttggc tttactgaat tggcgtgaat taaagcgagc cacggatggc
     4081 tggccattga taatctgaca ggcaaggccc ccaccatctt tcagcgcaag agtcccaccg
     4141 tgatcgaaca acgcacgggt ctcctctatg ctgatcccat gcagttccgc aaaatcatgg
     4201 agcccactgg tctcaatcaa tcgtccatca ggcatgacac attttcgctt aaaatgaggt
     4261 gaccaatctt ttcggaaatc aataaagtga atatttccac cacacgagat acccgagtcg
     4321 acttttgtta aatcaattcc taaatttttt tgccgcaacc gatcctgtaa ttcctgagga
     4381 agtcctcgat aaaaaacccg ctccatggct tcttccaacg tcacaccata actttgacgc
     4441 atgcgctccg ccgccgcata ttgctcaaga tccatgaccc acgtgtgttt cggatctttg
     4501 ccaagaggat ccacgcgaca cacgaatgcg ccttttgtaa ccagcgtacc ttcatgcgga
     4561 tagagataaa cccccccgtc ttgaagcacg cccgttatgt cttcaagagg aatatcataa
     4621 ctttcagaca ggactcgtgc atgcatctcc agatcttcgg gttccgtcat tccgcacacc
     4681 attacatttc cagggggatc ataatccgta tagttctcta gaatattata gagaaccgga
     4741 ggcttttctt ttttcaatgg acgttttttt actttaaaca ttcgatacac tccttcacgc
     4801 tgagttcttt tttggccttg gagttcttaa cgctcaaggg ggggggaggc aagattcgcg
     4861 caaaaccatc gcattccgat ttcactcgaa cattcccctt atatcaccat aatcttttcc
     4921 gcgccctgtt gcagatcttg aagtcgtaat ggtagaagga attcgcctat gactcaacca
     4981 taatgtccta taaacttcct atgcccaaaa attccctcat tcaggctttc catcagctgg
     5041 aagccttcaa atggaatcag caagaaggct ttcgcctcgc ctcaggaaaa accagcccct
     5101 attatgttga ctgtcgtatt gtattggctc atccgcactc acgtcatctt gtggctcaat
     5161 tagcctatca ccggcttaaa tccttagatt tcacgcttat tggcgggttg gaaatcggag
     5221 cgattcccct ggccacctgc atttcggatt ttggctatag agccgatcca cagcgggaat
     5281 ggcgaacgtt cgtcgtacgc aaacaaccta aggatcatgg attaggcaaa ttaattgaag
     5341 gcactatcca tcacggggaa acagctttag tggtcgacga tgtcttaaca acaggcggat
     5401 cattggtaaa ggccgcagaa tcagctcgcg ctcagggctt aaatgtgaca catgggttag
     5461 tagttgtgga tcgatccgaa gatgaaggaa aaagccatct ggcccacatg ggcattcaat
     5521 tactccccct tctcaccttg gaagatttaa gacaaacttc tccttctacc cggtagtcct
     5581 caaactattc cccccacggt tggacacctc agccaatcct tacagcctta acgggtaggc
     5641 atcctatttt tcaaacacga tccatctcaa attgatcgat gatgcccttt tcaagctcat
     5701 aaccttggcg ggactctcct attcgggata actcgcttga ccgtctctcc taaccaaccc
     5761 tacaatttta aaacaagagt tcaacaatag tctgctcaat atccttttca agtgggacaa
     5821 tgccattttc cttaacttcc tcaaaacaag aaagccatt
//
LOCUS       DAORAQ010000159         5848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_1984_length_5848_cov_59.803211, whole genome shotgun sequence.
ACCESSION   DAORAQ010000159 DAORAQ010000000
VERSION     DAORAQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1984_length_5848_cov_59.803211"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PKM72_11555"
     CDS             complement(<1..1319)
                     /locus_tag="PKM72_11555"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HNP61468.1"
                     /translation="MKVSALAGEPTQPSMLANIPRLVTAYYTGQPDPAVPEQRVAFGT
                     SGHRGSSLKNSFNEAHILATTQAICLYRQQQHIDGPLFLGMDTHALSEPALASTLEVL
                     AANGVIVMVDRDNGYTPTPVISHAILTYNRDRKTGLADGIVITPSHNPPEDGGFKYNP
                     PHGGPADGDVTTWIETQANALLADNLRGVKRISYEKARRSSTTHRHDYVGAYVGDLGA
                     VIDMEAIRDAKLSIGVDPLGGAGVDYWGPITERYGFPVTVVHEGVDPTFRFMNLDWDG
                     KIRMDCSSPHAMAGLIALKDRFDIAFANDTDHDRHGIVTRTAGLMNPNHYLAVAISYL
                     FTQRSGWRKDAAVGKTVVSSSMIDRVAAKIGRRLVEVPVGFKWFVDGLLDGSVGFGGE
                     ESAGASFLRRDGTVWTTDKDGIILDLLAAEMMAKTGRDPSQLYLDLT"
     gene            2389..3759
                     /locus_tag="PKM72_11560"
     CDS             2389..3759
                     /locus_tag="PKM72_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNP61469.1"
                     /translation="MDSLESFVSTVAGWVWGPPMLILLVGTGLYLTILLKGLQFRVLP
                     HALKLVFHKEPSANGDISHFAALMTALSATVGIGNIVGVAAAITLGGPGAVFWMWMTG
                     LVGMATKYSEAVLAVKYREQGEYGMRGGPMYYLAKGAGLPWLGWLFAVFTACAAFGIG
                     NMTQANAVAGIVESTFQIPPWITGVVLMSLTGLVILGGITSIGRFTSVLVPFMIVGYV
                     SSALVVLVLHVTEIPQALASIVYHAWNPIAAGGGFAGATMAAAMRYGLARGVFSNESG
                     LGSAPIAAAAARTDDPVRQALVSMTQTFIDTLVVCSMTALVILTATPWTQGIDPAQLT
                     SASFGETLGHTGELIVTCSITLFAYSTLIGWNYYGEKAIEYLMGTRAIVYYRVIFVGV
                     VLLGATSRLELVWNVSDIMNGLMAIPNLIGLLLLAKIIKQETVRYFSLQKMGAGSSRS
                     LPPPPV"
     gene            complement(3911..4261)
                     /locus_tag="PKM72_11565"
     CDS             complement(3911..4261)
                     /locus_tag="PKM72_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61470.1"
                     /translation="MGENRVFPSPLRHHEILLSELRNTFTINTLFVYTVRMALPSDLS
                     FSDSLKSDRRQAFYEHHKRIALAMILIVFLLPLVGVFMSGLSGAISGVVISVLSFYLA
                     PYALLKLGHWFVHS"
     gene            4474..4989
                     /locus_tag="PKM72_11570"
     CDS             4474..4989
                     /locus_tag="PKM72_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNP61471.1"
                     /translation="MVTKTQKAQAAQVTDMLRKDHKKVKGLFRKFEKTDNAREKQEIV
                     DMVVTELEIHAELEEKIIYPAVRSKMNDEDLMDEAIEEHHVVHGVIGELKKMKPGDQR
                     YDAKVTVLGELCKHHIQEEEEEMLPKAEKRDIDWDGLHQQVMKRKDQLMEKAGLSSNG
                     ASTNSKARKKK"
     gene            5001..5519
                     /locus_tag="PKM72_11575"
     CDS             5001..5519
                     /locus_tag="PKM72_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNP61472.1"
                     /translation="MRALNRMSNKNFNSPLIGRQSCRVTAWLPLRIMLLCLIPAVAIA
                     QGALGEELIVSGRTFSCPDRPNCVSTMAEAESHAIAPYRYQASLEEAKAELKHIFAQF
                     PRTELVREEEDYLQYEVKSFLFGFVDDVELWLDEATKTIHFRSAARSGYYDFGVNRKR
                     MEDVRQILNGKL"
     gene            5613..>5848
                     /locus_tag="PKM72_11580"
     CDS             5613..>5848
                     /locus_tag="PKM72_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61473.1"
                     /translation="MKKAASAVLAIFPCSRTGSTLCAQKCLWPSHRHDSGQGWTNFFE
                     PPPRPLIDNVPGGQFALGNYYYSALPLNKFIVIL"
BASE COUNT         1418 a         1466 c         1577 g         1387 t
ORIGIN      
        1 ttggtgaggt cgaggtagag ttggctggga tcgcgtcccg ttttggccat catttctgcg
       61 gctaacagat cgaggatgat gccgtcctta tcggtcgtcc acacggtgcc gtcacgacgc
      121 aaaaaggaag cgccggcact ttcttctcct ccgaagccga cggacccgtc aagcagccca
      181 tccacaaacc atttgaaccc gaccggcact tcaacgagac gccgacctat cttggcggca
      241 acccgatcaa tcatgctgct actcaccacc gtctttccga ctgcggcatc tttccgccag
      301 ccggagcgtt gtgtgaatag atatgaaatt gccaccgcga ggtaatggtt gggattcatc
      361 agccccgccg tgcgcgtaac aattccgtga cgatcatggt cggtatcgtt ggcgaaggca
      421 atatcgaaac gatcctttaa agcaatcagt ccggccatgg cgtgagggga ggaacagtcc
      481 atgcgaatct tcccgtccca gtccaggttc atgaagcgaa aggtcggatc cacgccttca
      541 tgcacgaccg tgaccgggaa tccgtagcgc tcggtgatgg gtccccaata gtccacgcct
      601 gcgccgccaa ggggatccac cccgatggac agtttcgcat cgcgtatggc ctccatatca
      661 atcacggcac caagatcgcc aacatatgcg ccgacataat catgtcggtg tgtggtcgag
      721 gaccggcggg ccttctcata ggaaatgcgc ttcactccgc gcaggttgtc ggccagtaag
      781 gcgtttgctt gcgtctcgat ccatgtggtc acgtcgccat cagccggccc gccatggggc
      841 ggattatatt tgaatccgcc gtcttcaggc ggattgtgcg agggtgtgat gacaatgccg
      901 tcggctaagc ccgtcttccg gtcacggtta taggtcagga tggcatggga aataacggga
      961 gtcggggtgt acccgttgtc acggtctacc atcacgatca caccattggc ggccagcact
     1021 tccagcgtac ttgccagggc cggctcggag agcgcgtgcg tatccatgcc taaaaagaga
     1081 ggcccgtcaa tatgctgttg ctgtcgatag aggcagatgg cttgggttgt cgccaggata
     1141 tgcgcttcat taaacgaatt ctttaatgaa gagccgcggt gtcccgatgt gccaaaggcc
     1201 acgcgttgtt ccggcacagc cgggtcgggt tgaccggtgt aataggcggt gaccagccgg
     1261 gggatgttag cgagcatgga tggttgggtg ggctctcctg caagggcact gaccttcatg
     1321 ttgatcgctc cgcgtccaga gatgaatgaa tgtgacatct cctgattttc aggaggaggg
     1381 ggcatacctt accgatattt atcaaaggat tcaaagcctt cattctttct catgccgacc
     1441 tggtggtgtc aaagaatcgg gtgcggcctt ctactctgtt tgggacctgt tcggcatggc
     1501 aggaatcttc aacgaaggct tcatgatctc agctcttatt tgagtcttat caacactttt
     1561 ttagaaattc tgtggataag aaaatcattg acatcggcat cttttcaacg gacatcaatg
     1621 ggcatcggtg tggagacaca tgtcaagaaa aaaatacgat ttctaccttt acgcctacgc
     1681 caaaaaaccc acaccactcc ctgtagtggt ataaattttt ttttatacaa tgcctaaaac
     1741 agtggcaatc tgctggtttc ctttttaatc cttagaaaaa catgcggctc acgcctgctt
     1801 tcaccaggtt atacacaaaa tttggggatg agtgtttttg gccatggtct gaattttgcc
     1861 gacctcaaaa atcaaggctt tttatgtgat ggctgccaat cgcgaggagt caaaggggtg
     1921 atggtgctca atcatctcaa aactattccc attcttgttc gtttccaacc ttttcgccag
     1981 cccttaatga attcgaaggg cgaaaattgt tgatgtgcca gtttgttctg accatacctt
     2041 acatcccgat atcctatgaa cgttgtcatc ctgacccatt cggcaaaact cagggcaggg
     2101 tgcaccgaag gatctcgctg agcttccgca tcgtcaacct gggcacagat gcttcgccat
     2161 ggactcagca tgacaagttg gaaagggtct tgcctggttc gggtgttcgt tgcaggtccg
     2221 aatgatgaag tgagatcatt cactttgaat gactgacgct ccttttcttg aagagccgtt
     2281 gcagaaccaa tcacatcata tttgtttaca caatgcacct cattggtaaa ataagcggtg
     2341 ctataccgtc accttcctgc tttttcttct accgggaaat aatgccgtat ggactccctt
     2401 gaatccttcg tgtcgaccgt agccggttgg gtgtggggtc cgcccatgtt gatcctgttg
     2461 gtcggcaccg ggttgtatct gaccattctg ctaaaaggcc tgcagtttcg tgtcctgccg
     2521 catgcgctga agttagtgtt tcacaaagag ccgagtgcca atggcgacat cagccatttt
     2581 gccgcgctca tgaccgcctt atcagccacc gtgggtatcg gaaatattgt cggcgtggca
     2641 gccgccatca cgctcggggg gcccggagct gtcttttgga tgtggatgac cggtctggtg
     2701 ggcatggcga ccaaatatag cgaagccgtg ctggcggtga aataccgtga gcagggcgaa
     2761 tacgggatgc gtggcgggcc catgtattac ctggccaagg gtgccgggct gccgtggtta
     2821 gggtggctct tcgctgtatt cacagcctgt gcggcatttg gcatcggcaa tatgacacag
     2881 gccaatgccg tggccgggat tgttgagtcg acctttcaga tcccgccctg gatcaccggg
     2941 gtggtgttaa tgagcctgac cggccttgtg atattgggcg gcatcacatc cattggccgg
     3001 tttacctccg tattggtgcc attcatgatt gtggggtatg tgtcatcagc cctggttgtc
     3061 ctggtcctgc atgtgacgga aatcccgcag gccttggcta gtattgttta tcacgcgtgg
     3121 aacccgattg cggccggagg cggatttgcc ggcgccacga tggctgccgc catgcgctac
     3181 gggttggcaa gaggcgtgtt ttcgaatgaa tcgggcctgg ggtctgcgcc tattgccgct
     3241 gccgccgcac gcacagacga tccggtcaga caggccctcg tcagcatgac ccagaccttt
     3301 atcgatacgc tggtggtctg cagtatgacg gcgttggtca tcctgaccgc gactccatgg
     3361 actcaaggta tcgatccggc tcaactcacc agcgccagct tcggggaaac ccttggtcac
     3421 acgggagaac tcattgtcac gtgttccatc acgctgtttg cctattcgac cttgatcggc
     3481 tggaattact atggtgagaa agccattgag tatctgatgg ggacgagggc aatcgtgtat
     3541 taccgcgtga tctttgtcgg ggtcgtcctg ctcggcgcca catccaggtt ggaactggtg
     3601 tggaatgtgt ccgatatcat gaacggcctg atggccattc cgaatctcat cgggttgctg
     3661 ctcctggcaa agattatcaa acaggaaaca gtacgctatt ttagcctgca aaagatgggc
     3721 gcaggttctt cgcgttctct tcctcctcct cctgtgtaat gcggggaagg gaaaactgaa
     3781 tacccccatc aaaccggccg atcaacaatc cgtgtgcgga ttattcacgc caccgactct
     3841 tctaaatttt gtctgtaggg ttgagggttg gtacctgtgt tcaccacagg ctgccaggtt
     3901 agatcgggta tcaggagtga acaaaccagt gccccaattt caatagggcg tagggtgcca
     3961 ggtaaaaaga caatacggaa atcacgacac ctgagatggc tccggacagt ccgctcatga
     4021 acaccccgac caggggaagc aaaaatacga tcaaaatcat ggccagggcg atacgcttgt
     4081 ggtgttcgta gaaagcctgg cgtcggtcac tcttgagcga atcggaaaat gataaatcgg
     4141 aaggaagtgc catccgtaca gtatagacaa acagggtgtt gattgtgaat gtattcctca
     4201 attctgacaa gagaatttca tggtgcctta aaggagatgg aaaaacgcga ttctccccca
     4261 tgaaccaccc catgcagacg aaagcgttcc acgcctccca aacgcacacg ctccctggga
     4321 ttcagacatc ctacaggccc ttgaacaaaa gatagtcaac ctcatccgtt ttatacattg
     4381 ggcgaatgac attctcctga tatagatcaa taagaaagat tgggcgaaag atgtattcat
     4441 aatattccta ataaggataa ggagaaattg tttatggtca caaaaactca gaaagctcag
     4501 gcagcacaag taaccgacat gcttcgcaaa gaccacaaaa aagtcaaagg gctttttagg
     4561 aaatttgaaa agacggataa tgcccgggag aagcaggaga tcgtcgacat ggtggtaacg
     4621 gagcttgaaa ttcatgcgga gttggaggaa aagattatct atccggccgt tcgctcgaaa
     4681 atgaatgacg aagacctgat ggatgaggcc atcgaagagc accatgttgt gcatggcgtc
     4741 atcggggaac tgaaaaaaat gaagccgggt gaccaacgct atgatgcgaa agtgacggtg
     4801 ctcggcgaac tctgcaaaca tcatattcaa gaagaagagg aagagatgct gcccaaggca
     4861 gaaaagcggg atatcgactg ggacggtctc caccagcagg tgatgaaacg gaaagaccaa
     4921 ctcatggaga aggcggggtt gtcttccaat ggcgcaagca cgaactcgaa agcgcggaaa
     4981 aagaaatagg aaaagcacac atgcgtgcgc tcaaccgcat gtcaaacaaa aacttcaaca
     5041 gtccattgat cgggaggcaa tcatgccggg tgacggcatg gttgccactc agaataatgc
     5101 tactgtgcct gatcccggcg gtagccattg cacagggagc actaggggag gagttgatcg
     5161 tgagtgggag gacattctca tgcccggaca ggcccaactg tgtttccaca atggctgagg
     5221 cggaaagcca tgcgattgca ccctatcgat atcaagcatc attggaggaa gctaaagccg
     5281 agctgaagca cattttcgct caatttcctc gcacggaatt ggtgagggaa gaggaggatt
     5341 atcttcagta cgaagtcaaa agttttctct ttggttttgt ggacgatgtc gagctgtggc
     5401 tggatgaggc cacaaaaacc attcacttcc gttcagccgc tcgcagcggc tactacgatt
     5461 tcggggtcaa ccgaaagcga atggaagatg tgcgacaaat cctgaatggc aagctatagc
     5521 aatagcaata agtatgcaat attctgcaac ctcagcataa agcccgtatg ggtcctcaat
     5581 gccatatctc ccaaacatgc attaaggggc agttgaaaaa agccgccagc gccgttctcg
     5641 ccatttttcc gtgctcacgt actggaagta cgctctgcgc gcaaaaatgc ctgtggcctt
     5701 cccatcggca tgactcaggg cagggctgga cgaacttttt tgaaccgccg ccgagacctc
     5761 tgatagacaa cgtgcctgga ggtcaatttg cccttggaaa ttattattat tcagcactcc
     5821 cattaaacaa attcattgtc atcttgag
//
LOCUS       DAORAQ010000160         5739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2036_length_5739_cov_57.958304, whole genome shotgun sequence.
ACCESSION   DAORAQ010000160 DAORAQ010000000
VERSION     DAORAQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2036_length_5739_cov_57.958304"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKM72_11585"
     CDS             <1..198
                     /locus_tag="PKM72_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNP61474.1"
                     /translation="SVMILNKIDLLPHLTFDVKTCLEFAHKVNPKLQVFQLSAITGEG
                     LQHWYDWVTSQIKNSKKQVIR"
     gene            237..671
                     /locus_tag="PKM72_11590"
     CDS             237..671
                     /locus_tag="PKM72_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61475.1"
                     /translation="MEADHAAAVATLATKTQCVAHTLKQGLTWGLGHTITLLLCGSMV
                     FLPESAVPERLANWLELVVGAMLVGLGGDVLRKIIQERIHVHEHHQQPPHLHAQSHRG
                     KLNHRKTDHQHSHSTAFPYRTLFVGLMHGMAGSAALIFLSLH"
     gene            complement(951..2837)
                     /gene="acs"
                     /locus_tag="PKM72_11595"
     CDS             complement(951..2837)
                     /gene="acs"
                     /locus_tag="PKM72_11595"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNP61476.1"
                     /translation="MSENIETLQRSTRVIPPSPDITANAHIQDYETAYMQSVVNPEKF
                     WDGIAKELHWFSPWNKVLEWNYPWAKWFVGGTCNIAYNCLDRHVQTWRKNKVAVIWVG
                     EEGQERILTYGELYRQVNKCANGLKSLGLNKGDRVMIYLPKIPEQIVAMLACARIGVI
                     HSVVYSGFSATALAARIEACEARVVITADVGYDRSRKIPLKPVVDEAVAKCLSVEKVI
                     VVQREQSASPLQAPKELDWEDWLKDQSPKCEAEALDSETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYITTKYVFDLKDEDVYFCVADPGWVTGHSYIVYGPLLNGATILTAEGKP
                     DYPNPGRWWDLIERYGVSIFYTTPTAIRLLMKFGEDWPKKYDLSSLKILGSVGEPINP
                     EAWEWFHRVTGGTKPIMDTWWQTETGMIMVTPLPCVPLKPGSATRPFLGIEADVMDRK
                     GNTVEHGGGFGVIKKPWPSMMRTIYKEPDRYLLYWNTIPGVYTAGDVCHKDEDGYLWF
                     MGRADDVVKVAGNRIGTAEVESALVSHEAVVEAAVIGKPHQTAGEQIKAFVILKQGQE
                     ETPDLIKSLQDHVLQELGKIAVPREIEVVPSLPKTRSGKIMRRVLKAKELGQDPGDIS
                     TLEE"
     gene            complement(3097..>3741)
                     /locus_tag="PKM72_11600"
     CDS             complement(3097..>3741)
                     /locus_tag="PKM72_11600"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNP61477.1"
                     /translation="PCKSHPWHGVEIGAEAPFTVTVYVEIVPTDTVKYELDKKSGHLR
                     VDRPQRYSNVCPTLYGLIPQTYCGQSVAEFSAGCTNRPGLVGDLDPLDICVLTEKSII
                     HGDVLLQAIPIGGLRMIDGDEADDKIVAVLKGDALHEQWTDIKACPVHFVERLMHYFL
                     TYKDAPGSQKKNCEITHVYGREEAHEVIRRSQQDYAAKYGHIQAQLAKPRHALN"
     assembly_gap    3742..3841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3842..5127)
                     /locus_tag="PKM72_11605"
     CDS             complement(<3842..5127)
                     /locus_tag="PKM72_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNP61478.1"
                     /translation="MFTPEDHLELIRRGISTFQVESQLQRLRHGVPPITIIRPCRVND
                     GIIQLDPEHFPQYQQQFEAARQADRISKFIPASGAATRMFNDLLKFLSEEVSSEFRSN
                     QTSTLPHAVDQAWTRLQDFPFISDLEKYFHWQGQPPPTDQHTHDLNTILQAVLKTPGL
                     GYAELPKALLSFHRYEEGPRTALEEHIYEALRYQPDQIHSIKIHLTVSPQYEQAIEDH
                     LHTIHQTLAREGWKLNCTVSFQKPSTDTIALDSGNQPFRAEDGTLVFRPGGHGALLEN
                     LNAYQGDIVFISNIDNVVPDHLKDPIVAWRKALGGYLVDLQQHVFQHIEQLSSVPVDA
                     KSVQQAEACILQELLLPLPQSYRELALPDQATLLKQYLDRPIRICGVVPNTGDPGGGP
                     FWVAHPEDAPSRQIVEQSQVNPDSEAQQKLFASGTH"
     gene            5304..>5739
                     /locus_tag="PKM72_11610"
     CDS             5304..>5739
                     /locus_tag="PKM72_11610"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNP61479.1"
                     /translation="MIPGFKTFQGLAALGVFLLGISSGCSIKATSDTTSDATTNVLSS
                     TSGKSWWTEDGLVKHGEHARAFVAVNYDTLLQEIAQGHGEYVQAFGTILGVQSQQQAH
                     FQELIQNEYPLLVEIPISLGDDQLNRFIAHAQQAGTHAASSNL"
BASE COUNT         1373 a         1348 c         1436 g         1482 t
ORIGIN      
        1 agcgtcatga tcctcaataa aatcgatctt ctgcctcacc tcacctttga tgtcaaaaca
       61 tgcctggaat ttgctcataa ggtgaatccc aaacttcagg tgtttcagct ttcagccatt
      121 actggcgagg gacttcaaca ctggtatgac tgggtgactt cacagatcaa aaattcaaaa
      181 aaacaagtca ttcgctgatg tttattttat tgaggcttct tggaaggaga cttgccatgg
      241 aagccgacca cgcggcggct gtcgccacac tcgcgaccaa aacccaatgt gtggcccaca
      301 cccttaagca gggactaaca tgggggctgg gtcacactat cacgttactg ttgtgtggct
      361 cgatggtgtt ccttccggaa tctgcggttc cggagcgatt ggctaactgg ctggaattgg
      421 tcgttggcgc catgctggta ggactgggag gcgatgtatt acggaaaatc atacaagaac
      481 gtatacatgt tcatgaacac catcaacagc ctccgcacct tcatgcccaa tcccatcggg
      541 gaaaactcaa tcaccgaaag acggaccatc aacattcaca ttcaaccgca tttccctacc
      601 ggacgttgtt tgtaggattg atgcacggaa tggcggggtc cgccgcactc atatttctgt
      661 cactccatta aacggtttct ccgctaccag gaattatcta catttctata ttcggtcttg
      721 gatcaaggtt ggaatggcca cattctcgat gggcatcgcc atccctctcc gatattcatc
      781 ccgctcaata cggtggctgc caaggttctc ttgctgacct tagttttaat ttcgtttttc
      841 agtttccgat cgcttcaata cagggaagcg gtggtcaatc cgctgattag aaatcagcgg
      901 gatcaggaaa aaagagtttc tcgaaaaatg aagaatgaaa taatctggct tcactcctcc
      961 aatgtagaaa tatctccggg atcctgaccg agttccttgg cctttaagac gcggcgcatg
     1021 attttgccgg agcgggtttt gggaagcgag gggaccactt caatttcccg ggggacggcg
     1081 atcttgccca attcctgtaa gacatggtcc tgcaaacttt taatgagatc gggcgtttct
     1141 tcctgccctt gcttcaagat gacaaaggct ttgatctgtt ccccggcggt ctgatgcggt
     1201 ttgcctatga cggcggcttc cacaaccgct tcatgactga ctaaagcgct ctcgacttct
     1261 gccgtcccga tgcggtttcc agcgactttt accacatcat cagctcgacc cataaaccat
     1321 aagtagccgt cctcatcttt atgacacaca tcgcctgcgg tatagacgcc ggggatggta
     1381 ttccaataca aaagataacg atccggttct ttgtaaatgg tccgcatcat tgacggccag
     1441 ggttttttga tgacgccgaa tcctccacca tgctctacgg tattgccttt gcggtccatg
     1501 acatcggctt cgatgcctaa aaacggacgg gtggctgaac cgggtttgag tggcacacaa
     1561 gggaggggtg tgaccatgat catcccggtc tctgtttgcc accaggtatc catgatgggt
     1621 ttggtgccac ccgtcacgcg atggaaccat tcccaggctt ccggattaat aggttcgccc
     1681 actgatccta aaattttcag ggaagagaga tcgtattttt ttggccagtc ctcgccaaat
     1741 ttcatcagca gccggatagc cgtcggcgtg gtatagaaga tcgatacccc atagcgttca
     1801 atgagatccc accagcgtcc gggattggga tagtcgggtt taccttctgc cgttaaaatc
     1861 gtggcgccgt ttaagagcgg accatagacg atgtagctgt ggccggtaac ccaccccgga
     1921 tcggctacgc aaaaatagac atcttcatct ttgaggtcaa agacatattt cgtggtgata
     1981 taagtaccca ccatatagcc gccatggaca tggaccacgc ctttaggttt tcccgtggtt
     2041 cctgaggtat agaggatgta caagggcgtt tcggagtcta acgcctcagc ctcacatttt
     2101 ggtgattgat ctttgagcca atcctcccaa tccaattctt tgggagcctg aagaggggag
     2161 gctgattgtt ctcgttggac gacaatgact ttttccactg agagacattt ggcgaccgct
     2221 tcatcaacga ccggcttaag gggtattttt ctactacggt catatcccac atctgcggtg
     2281 atgaccactc gcgcctcgca cgcttctatg cgtgcggcca acgccgtggc gctaaagccg
     2341 gaatagacca ccgaatgaat gaccccgatg cgggcacagg ccagcatggc gacgatttgc
     2401 tcggggattt tcgggagata aatcatgacc ctgtcgccct tgttgaggcc caagcttttg
     2461 agaccattgg cgcacttgtt gacctgccga tataactcgc cataggtcag gattcgttcc
     2521 tgaccttcct ctccgaccca aatgacggcc actttgtttt ttcgccaggt ttggacatgg
     2581 cgatcgagac agttgtaggc aatattgcag gtgcctccca caaaccattt agcccaggga
     2641 taattccact ccaacacttt attccagggg gaaaaccagt gaagttcttt ggcaatgccg
     2701 tcccagaact tttccgggtt cacgacagac tgcatgtacg cggtttcata atcctgaata
     2761 tgagcgttgg ctgttatgtc gggggaggga ggaatgacgc gggttgatcg ttgcagggtt
     2821 tcaatatttt cactcattag gtctgttcct ccttagttac gcattttaga acatcaccaa
     2881 gaacgtgtct tctcagcgaa ttggctgaca agcccattat gctgcccttg gctcgttgct
     2941 tcaagagggt aggaatgaaa gtccgtgata aagagatgga aagtgaataa ggattggagg
     3001 cgatgaatat gagaaccgtc gctgaccgaa agctggttgc cattttttga aaaacgagat
     3061 tggggaaatt caatgggttg agagggactg gttatctcaa ttaagagcgt ggcggggttt
     3121 tgcgagttgg gcctgtatgt gtccatattt ggcggcgtaa tcctgttgac tccgacgaat
     3181 cacctcatgg gcctcctccc gaccatagac atgggtgatt tcgcaattct ttttttgcga
     3241 gccgggagca tctttgtagg tcaggaagta atgcatgagg cgttcgacaa agtggaccgg
     3301 acaggctttg atatctgtcc attgttcatg gagggcatcg cctttgagga ccgccacaat
     3361 tttatcatcg gcttcatccc catcgatcat acgcaagcca cctatgggaa tagcttgcag
     3421 caacacgtct ccatggataa tgcttttctc cgtcaggaca caaatgtcta atggatccag
     3481 atcgccgacg aggccaggcc gattggtaca accggcgctg aattccgcga ctgattgacc
     3541 gcaataggtt tgaggaatta atccgtacag cgtggggcac acattggaat agcgctgagg
     3601 ccgatctacg cgcaaatggc ctgatttttt atccaattca tatttgacgg tgtcggtggg
     3661 tacaatttcg acataaaccg tgacggtgaa tggtgcctct gctcctattt cgacaccgtg
     3721 ccacggatga gatttacagg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 naaatgggtt ccagaggcaa acaatttttg ctgagcttca gaatccggat tgacctgtga
     3901 ttgttcgacg atttgcctgg atggggcatc ctcagggtgg gccacccaaa atggtcctcc
     3961 gcccggatcg cccgtattgg gtacgacacc acagatccgg atgggacgat ctaaatactg
     4021 tttcaataat gtggcttgat cgggaagtgc caattctcga tacgattggg gaagcgggag
     4081 aaggagttcc tgcaggatac aggcttcggc ctgctgaaca ctttttgcat ctaccggaac
     4141 tgagctgagt tgttcgatat gttgaaacac atgttgttga agatcaacca gatagcctcc
     4201 caaggccttt ctccatgcca cgatcggatc tttcaaatgg tcagggacga cattgtcaat
     4261 atttgaaatg aatacgatgt ctccctggta ggcattcaaa ttttccaata aggcaccatg
     4321 tcctccaggt cgaaaaacca gagtgccgtc ttcagctcga aatggctgat tgccagagtc
     4381 caacgcgata gtgtcggtcg acggtttttg aaaggaaacc gtgcaattga gtttccaccc
     4441 ctctcgggcc aaggtctggt ggatggtgtg gagatggtcc tcaatggcct gttcatattg
     4501 aggagaaacg gtcaaatgaa ttttaattga atgtatttga tcgggctgat atctaagggc
     4561 ttcataaata tgttcttcca gtgcggtgcg tgggccttct tcgtatcgat ggaaagagag
     4621 cagcgcctta ggcaattcgg catagccaag tcccggcgtt ttcagtaccg cctgaagaat
     4681 cgtgttgaga tcgtgtgtgt gctgatcggt aggagggggt tgcccctgcc aatgaaaata
     4741 cttttctaag tccgatataa atggaaaatc ctgcaagcga gtccatgcct ggtcgacagc
     4801 atggggaaga gtcgacgtct gattggaacg gaattccgat gagacctctt cggacaaaaa
     4861 tttgaggagg tcattgaaca tacgagtggc ggctccggac gcaggaataa atttactgat
     4921 ccgatccgcc tgccgggcag cttcaaattg ttgttgatac tgcgggaagt gttcgggatc
     4981 aagttgaata ataccatcgt tcacccggca tggacggatg atggtgatcg gtgggactcc
     5041 atgacgcaaa cgctgtagtt gtgattccac ttgaaatgtg gaaattccac gacgtattaa
     5101 ttcgagatga tcctcagggg taaacatagg gttatgtcta tctcaatgtg tctcgagaat
     5161 gttcggagag atcacaccag acaggagttg tgtttcagga tgaaaaatgt caaaattttg
     5221 atgttctcga tccgacgaat atccaattgt aaagtgtcct gccggctatg tcatgttcat
     5281 gaattaagtt aaggagaata atcatgatac caggcttcaa aacgtttcaa ggtcttgcgg
     5341 cattaggggt ttttctgttg ggaatttcat ccggttgttc catcaaggcg acatccgata
     5401 ccacttcgga tgccaccacc aatgttttgt cctccacctc cggcaaatcc tggtggacag
     5461 aagatgggct tgtcaaacat ggggaacatg ccagagcctt tgtggcggtt aattatgaca
     5521 cgctccttca agaaatagcc caaggtcacg gggagtatgt ccaggccttc gggacgatat
     5581 taggagtcca atctcaacaa caggcccact ttcaggaatt aattcagaac gagtatccct
     5641 tgctggttga gatacctatt tcacttgggg atgatcagtt aaacaggttt atcgcccatg
     5701 cccagcaagc aggaacccat gctgcatcct caaatctct
//
LOCUS       DAORAQ010000161         5702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2051_length_5702_cov_67.779529, whole genome shotgun sequence.
ACCESSION   DAORAQ010000161 DAORAQ010000000
VERSION     DAORAQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2051_length_5702_cov_67.779529"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKM72_11615"
     CDS             <1..561
                     /locus_tag="PKM72_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNP61480.1"
                     /translation="TMAKHRDLSGDEATALLRMVGEYSRALDLLDDYDHQRVPAPRAR
                     SQTRYTLTYQEAIDIVARLRERFGSSALFGQEKDESLHSSLQAIMQTFGGQDLYSSLE
                     EKAAHLLYFLVKNHSFVDGNKRIAAALFLWFLERNSVLTNATGTRLISDAALVAMTLM
                     IAESHPEEKDVLVRIVMHLLCQHDPL"
     gene            1302..>4402
                     /locus_tag="PKM72_11620"
     CDS             1302..>4402
                     /locus_tag="PKM72_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP61481.1"
                     /translation="MSPHTRDTGLETLWEEIRRFMGVCPSFFRLASPEPIVARGLFDL
                     AKFAYLESPLPSSFKEKLFTYLSRFCGVRYCVTRHAAFLLGRGNIAGDPTCPPLTPEE
                     VITLLSEPFPEDEQLSDTLSALATMGAPLKDWPAHDSMVERHLRIACAMIFLEPGHST
                     AWCTALRRLLGPSQFEWLLLFLAFVRTAHFWTEVHASYDAVIPMASEQPSPGDDAVID
                     QTPQLVFEEDIELLFREQQALVEPLLQKMQDATRFELGSQLHAELQELRTTHRLAQAL
                     KKSEALLRDLYENAPDMFFSVDAETERVVECNRTLLEKTGFSREEVIGRSVFERYTKA
                     SLPNALKSFEHFRQYGVEHNAELQLRTREGKTIDVSLNTTAVRDASGRIISSRSIWRD
                     ISQQKSLEDQRRLIFEASPNGMLVVNQKGHILFANSRIEEWFGYPREELIGKPFEGLI
                     PAQFRVQQAGERLDFVLIPYVESMEPDPEFRGRGKDGRTIPLTIRLDPMGKEDDETTL
                     VTMTNITERQRDRETIRQSTIALHEQLSLNKTITDNTSTSLLMIDTAGRVTFANPSTE
                     IVMGFKPEELIGRVLHEMVHHTRPNGFPFPIEECPLAGISLLRETLTNHEDVFIHKDG
                     HFYPVRCNTRQIFKQGERIGNVIEVLDITDLKRAEKDRQLFTLELARQVNDRTEELVN
                     SQKLLRELAAALNLTEQRERQRIATELHDYLSQLLVVCRLKLGQIRPAPGMDSESSDL
                     VKETEEVLDQALTYTRTLVAELSPSVLFEFGLLAALQWLAGQMIHHGLKVEMSCEGIQ
                     DWKVPEEQAILLYQSIRELFLNVIKHADTDRATLTLRKEEGVLRISVRDEGKGFHPPK
                     GTATQPFTDQAMKFGLFSIRERMITLGGTFEIESLPDHGTTARLLVPIALKLEKSSTP
                     QQDADGQDIDPTRVDHSFPDAGDLPKISTKNQIRILIVDDHVMVREGLRKMLESHEDL
                     ELVGEAGNGEEAIDLVERLKPALVIMDVNMPRLNGIEATSRIKAHHPDIIIIGLSVNA
                     GGE"
     assembly_gap    4403..4502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4503..4814
                     /locus_tag="PKM72_11625"
     CDS             <4503..4814
                     /locus_tag="PKM72_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61482.1"
                     /translation="FVKSRIEIGEMMTNYFKGGKGYGDGQRPSPEAMKEMGADINTKL
                     SALLAKHDLTIEEYRQRSPEVFADEAAVNGYLNEHPDLKARYDALPLGSMKGGSTGRG
                     Y"
     gene            4971..5180
                     /locus_tag="PKM72_11630"
     CDS             4971..5180
                     /locus_tag="PKM72_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61483.1"
                     /translation="MKLKTSDPSFIDSRRPSQLGERLAKHKIDAARKLTPEQRLLLAL
                     ELSDAAAALQLACSQKRYPTFFPAL"
BASE COUNT         1425 a         1465 c         1417 g         1295 t
ORIGIN      
        1 accatggcga aacacaggga tctttccggc gatgaggcca ccgcgctcct ccgcatggta
       61 ggcgagtata gccgggcact cgacctgctt gacgattatg accaccaacg ggtgccagcc
      121 cccagggcca ggagccagac acgttacacc ttaacttatc aagaagccat cgatatcgtg
      181 gcgcgcctcc gcgagcgctt tggaagctca gccctcttcg gtcaggaaaa agatgaaagc
      241 ctgcacagta gtcttcaagc gatcatgcaa acgttcggtg ggcaagatct ctattcaagc
      301 ttggaagaga aagcggccca cttattgtat ttcctggtga aaaaccactc ctttgtggat
      361 ggcaacaagc gtatcgcggc tgcgttattt ctttggtttc tggaacgcaa cagcgtactg
      421 accaatgcca ccgggacgcg tttgatttcg gatgccgcac tggtagccat gaccctgatg
      481 atcgcggaaa gccatccgga agaaaaggac gtgttggtac gcattgtcat gcacctcctc
      541 tgtcaacacg atccattatg agcaccgaca cagcagacac tttctgtgcc gattgccaca
      601 ctagacgtta gacggcaatg ccagatggat ttggctgggc tgtaccgctt taatattttc
      661 tcgttctgaa aggaattcat gaccatggat ttgacaccca atatgaccgg agtttctttg
      721 actgttgtac tccacgcaga gaaaagtctg gcgcttggct ggacactcaa ttgaaaattt
      781 gtcgggcaag tgcgtgcctc attccctata caaaattcgg cttgtggctc cttttatatc
      841 tactgtctcg taattcccac cctgggtaaa gcatgttggg cagggaccac ggtcaccctc
      901 attttacctt tatcactgtg agtgaaaagg gagcttactc ttacattaga acccagctgg
      961 aggcttaaca agcacagcac cctctcccaa aactcctttt gatacaccac ttccttgcct
     1021 caaatgtaaa aagtgcctct actcagcacc tgtacaaaat gggtcaaaaa aacctgattc
     1081 cgatcttcgc tgacccgggc agcgggtgca tcccctgaaa acgctaattt caacagccct
     1141 tcatcggcaa aattcgatct ccacaatttt gaagatgagt cccctgagat ggattcggat
     1201 gtgttggatg tggcagtttg gctttgtccg atcccctcca agccttgtat aatcggacga
     1261 caagacgccc gataccattt cactaaaatt aggaataaca ggtgagccct catacccggg
     1321 ataccggctt agaaactctt tgggaagaaa tccgccgatt catgggagtc tgtccgtcct
     1381 ttttccggtt ggccagccct gagccgatcg tcgcccgtgg ccttttcgat ctggcaaaat
     1441 tcgcctatct ggaaagccca cttccctcaa gcttcaagga aaagctcttc acctatcttt
     1501 cccgattctg cggggtccgg tattgcgtga cacgacatgc cgcatttttg ttaggacgag
     1561 ggaacatcgc cggcgaccca acctgtcctc ctcttacacc cgaagaagtc atcaccttgc
     1621 tcagcgagcc gtttcccgag gacgaacaac tttccgatac cctgtccgcc cttgcaacca
     1681 tgggtgctcc cctaaaggac tggccggctc acgattcgat ggtggagcga cacctgcgca
     1741 tcgcctgtgc gatgattttt ctggaaccgg gacactccac cgcatggtgc actgcccttc
     1801 ggcgactttt gggaccttcg caattcgaat ggcttctgct ttttctcgcc tttgtgcgga
     1861 ccgcgcattt ctggaccgaa gttcatgcat cgtatgatgc cgtgataccg atggcgagtg
     1921 agcagccttc cccaggggat gatgccgtca tcgaccagac tcctcaactg gttttcgaag
     1981 aggacataga acttctcttt cgagaacagc aagcactggt cgaacctctc ctgcagaaaa
     2041 tgcaggacgc cacacggttc gaactcggct cacagcttca tgccgagttg caggaattac
     2101 gcaccactca ccggctcgca caagccttaa aaaaaagtga ggccctcctt cgcgacttat
     2161 acgagaatgc cccggatatg tttttttccg ttgatgccga gacagagcgc gtggttgagt
     2221 gcaatcggac ccttctggaa aagaccgggt tttcccgcga agaggtcatc ggcagatccg
     2281 ttttcgaacg gtataccaag gcctctctcc cgaacgccct gaaatccttt gaacacttcc
     2341 gccaatatgg agtcgaacac aatgcggaat tgcaattgcg gactcgtgaa ggaaagacga
     2401 ttgatgtcag cctgaatacc acggccgtgc gtgatgcgtc tggacgtatt atctccagcc
     2461 gctcgatctg gcgggatatc agccaacaaa aatcccttga agaccaacgg cgtctcatat
     2521 ttgaagcctc ccccaatggc atgctggtgg tgaaccagaa gggacacatt ctttttgcca
     2581 attcacgaat agaggaatgg ttcggatatc cccgcgagga gctaattgga aagccatttg
     2641 aaggtcttat tcccgcgcaa tttcgagtcc agcaggccgg cgagcgattg gacttcgtgt
     2701 tgattcccta cgttgaatcc atggaacccg atcctgagtt tcgtgggcgt ggtaaagacg
     2761 gaaggaccat cccactcaca atcaggctcg atccgatggg gaaggaagac gatgaaacga
     2821 cattggtgac gatgacgaat attaccgaac gccaacggga cagggaaacc attcgccagt
     2881 cgaccatcgc gcttcatgaa caattgtctc tcaacaaaac catcaccgat aatacctcga
     2941 ccagcctgct tatgattgat accgccggac gagtgacctt tgccaatccc tccacggaaa
     3001 tcgtgatggg gttcaaacct gaagagctga ttggccgggt tttgcatgaa atggtccacc
     3061 atacccgtcc aaatggattc ccatttccca tagaagaatg tcccctggcc gggatatctc
     3121 tcttaagaga gactctgacc aatcatgaag acgtattcat ccataaagat ggccattttt
     3181 atcctgtacg ctgcaacacc aggcagattt tcaagcaagg ggagcggata ggtaatgtca
     3241 tcgaagttct ggatatcaca gacctgaaac gggccgagaa ggaccggcaa ctttttacgc
     3301 tggaactggc gaggcaggtc aatgatcgaa ccgaagaact cgtcaattct caaaaactgt
     3361 tacgggaatt agcggccgcc ctcaatttga ccgaacaacg ggaacggcaa cgaattgcga
     3421 cggaacttca tgattacctc agccaactac tagtggtgtg ccgcttgaaa ctgggccaga
     3481 tcagaccagc ccccggcatg gactctgaaa gctccgatct ggtcaaagaa accgaagagg
     3541 tcctcgatca agccctcacc tacacaagaa cgctcgtcgc ggaattaagc ccttcggtct
     3601 tatttgaatt tggattgctg gcggccttgc aatggttggc cggtcaaatg atccatcatg
     3661 gcctgaaagt ggaaatgagc tgtgagggca ttcaagactg gaaggtcccg gaggaacagg
     3721 cgattttgtt gtatcaatcg atccgcgaac tgtttctcaa cgtgataaaa catgccgaca
     3781 cggaccgcgc cacactcaca cttcgcaaag aggagggggt attacgtatt tccgtccgcg
     3841 atgagggtaa ggggtttcat ccccccaaag gaactgccac ccagcctttc acggaccaag
     3901 cgatgaaatt tggcctgttc tcgattcgcg aacgcatgat tactcttgga ggaacattcg
     3961 aaattgaatc actgcctgat cacggaacca cggcccgcct ccttgtaccc atcgcgttaa
     4021 agttggaaaa gagttctaca ccacaacagg atgcggacgg gcaggacatc gatccaacca
     4081 gggtcgatca ttcgtttcct gatgcaggcg atctcccaaa gatcagtaca aaaaaccaaa
     4141 ttcgcatttt gatcgtcgat gaccatgtga tggtgcggga gggattaagg aaaatgttgg
     4201 agagccatga agatctggaa ctggtaggtg aagccgggaa cggcgaagaa gccatcgatt
     4261 tggttgaacg cctgaaacct gccctggtga tcatggatgt caatatgcca cgactgaatg
     4321 ggattgaggc cacatcgcgg ataaaagcgc atcatccgga tatcattatt atcggattat
     4381 ctgtcaatgc cggcggagaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nntttgtgaa gtcccgtatt gaaatcggcg agatgatgac gaactatttt aagggtggaa
     4561 agggatatgg agacgggcag cgaccctcac ccgaagcgat gaaggaaatg ggcgcggata
     4621 tcaatacaaa actgagtgcg ttattggcaa agcatgattt gacgattgag gaatatcgcc
     4681 agcgcagccc tgaggtgttt gccgatgagg cggccgtaaa tgggtatctc aacgagcatc
     4741 ctgatctgaa agcacgctac gatgctctcc cgttgggcag catgaaaggc gggagcaccg
     4801 ggcgggggta ctgagacctc ccatgatttg taagtagtac tctgctgttg gaaaaattca
     4861 acgggcgcta tgaatgtgac caaggcacgg gggcatgatt acacctgtcg gtaacgatgc
     4921 caatccattt ttccagtgac gaatccgatg tttccatgta agatattttt atgaaattga
     4981 aaacgtccga tccaagtttt attgactcac gccgtcccag tcaacttggc gaaaggctcg
     5041 cgaaacacaa gattgacgcg gctcgaaaac tcacccctga acagcggttg ctcctcgctc
     5101 ttgaactgtc cgatgcggca gccgctctcc aactcgcgtg ttcccaaaaa aggtatccga
     5161 cattttttcc cgctctatga atatctgcca cagaaccata tttccggggg aaagcggaga
     5221 ccattcagga tttatggttt tggccttata gctttatgat aagtatcgga aatccaattt
     5281 aggggtggat ctctggtagg tgaatctctc gtacgtatgt acccggtttt cgtgtcaaag
     5341 tgatgttctg tattgccggt tctcggcccg gcagccgagc cccttttgtt tcggcaaaag
     5401 gagccaaaac cattgacgcc ccgtccggcc ttattagacc gaacggatgc cagcctgtgg
     5461 aggcggacca actcgcggag tttatcctga gccatgtcga aggactcaaa caaggctcgc
     5521 tggcacatga gagcgtccat ctagagggcc ggacggcagg cgtcggaaca ggggaaacga
     5581 acctttccag aacacatatg aaagagcgag gaacccttta cacggaggta gttgaatccc
     5641 atgaacgatg agcagccggc taaggtctgc gccagtctca tagagggtat gcagagtctc
     5701 ca
//
LOCUS       DAORAQ010000162         5611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2096_length_5611_cov_69.579374, whole genome shotgun sequence.
ACCESSION   DAORAQ010000162 DAORAQ010000000
VERSION     DAORAQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_5611_cov_69.579374"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1429
                     /locus_tag="PKM72_11635"
     CDS             203..1429
                     /locus_tag="PKM72_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61484.1"
                     /translation="MQSLKRMKQNSVIVMGSTIIAIFIMEALLRGIGVPSKLNSGWGW
                     ENSAGRKLSKYDALTTNQFGYRGQNINYDADDYVVLLVGDSQVEAYAGRPEHMPEQFL
                     QEFLTSQLHKPVKVFSLAASGWGQDQQLLAIQEYFRVYRADLVLLWATPGNDFWENAF
                     PDRSVTPLAGPLKPTFRLIDGKLSGPYFTSESYLHNSAMAQLIETVMANMQQETLEQR
                     ILRRWLQKMPSSHTTHKPDNENVCGGLTVVNQVEFFNTIFDLNKDIGYTVKSGQDVVN
                     SRSLFSAYMLDPSNRDEYLVAITESLLWHVKTEVKKNHSEFLAFYPVREDFDKRGMQM
                     VRCVADFQGNIFRVSLDFTSRLQDVISSEDLVVFDLLGGNEIVVSPEDRHLNDFGNEL
                     VMKKLSLTLMERSIFN"
     gene            1653..2552
                     /locus_tag="PKM72_11640"
     CDS             1653..2552
                     /locus_tag="PKM72_11640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNP61485.1"
                     /translation="MEGIPTTQKLCSIIIVTYNSSSCIGACLSSLLNISDIELVVVDN
                     DSKDGTAAKLQKEFPQITLIALQNNIGFGRACNIGLAASSGSFALFLNPDTIANEKAI
                     RTLVQFYEKHPRVGIIGGRLVDPCGKPLQSMGDTPSLTGLVLDKPLAWVAKRVGAQGV
                     FRRILGRCSAKFLMPPEAEPVAWVSGAFLCCRRSIWDTIGGFDENFFLYYEDVDLCLR
                     AKQAGWEVWHVPEAVVEHQSGASFGGDLFKQKEIYYANQYYFFRKHFGRPIAWALWIL
                     QSVYSRLGMYRRLGTDRIGRGLL"
     gene            2602..3672
                     /locus_tag="PKM72_11645"
     CDS             2602..3672
                     /locus_tag="PKM72_11645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP61486.1"
                     /translation="MRVLQIVADGKPGGGTTHVLQILRGLRHAVSFQLITEEQSYLMK
                     EAGALGIPCQGLRFFISRLNFALPFHLRALVLALRPDLVHVHGGRAGFFFALSLLKIP
                     MVYTVHGFHFIEKSPRIRRMALMAERWNFRRACHSIFVSKYDVTLAENSRLLSGCDKR
                     VVIYPGFSLKNLPKAHPRTLLHIGFLGRLEYQKDPLLFLEMMAYLPEYSATIVGDGAL
                     APMIKQEIAKRGLCGRVHMVGELSHGEALEILATFSVVVLTSRWEGLPVLVLESMGIG
                     VPVVSMNVSGLEEIIHHEVNGMLVEKRRGEDLADKVRIITHNEGLRAFLIKNAQATIH
                     EEFSMDNMLGSILKIYQEASVA"
     gene            3695..4879
                     /locus_tag="PKM72_11650"
     CDS             3695..4879
                     /locus_tag="PKM72_11650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP61487.1"
                     /translation="MKVALVHDWLTGMRGGERCLEALCELFPDAPIYTLFHVKGSVSD
                     TIERHPIIPSFLNRIPFAKSRYRYLLPFFPSAIQRLEIQQYDLVVSSSHCVAKGIRVP
                     RETCHISYVHTPMRYIWDGFDTYFGNAGFWNVGRLGMALFRARLQQWDMESNAHVSRF
                     IANSHNVAERITRQYGRPAYVVYPPVDWQGHQVSRCHEGFFLMVTAFAPYKKVDLAIA
                     AANELEIRLKIIGQGQDEKRLKRMAGPTVEFLGWQPDHRVRDFYSRCLAVLFPGEEDF
                     GIVPLEAMATGKPVIAYGKGGALETIVPLNPLPKLGENHLERGAQTRESHSGLTGVFH
                     YEQSVQAIIEAIQLFKQHLTEFDPDAIRDHVEPFDRSHFKEQVQQVIMSAYHEFRRVP
                     LC"
     gene            4873..>5611
                     /locus_tag="PKM72_11655"
     CDS             4873..>5611
                     /locus_tag="PKM72_11655"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNP61488.1"
                     /translation="MLKRHSEFLKNLLFLSDLVVICVCWVGAYFIRFSVPLFPITKGI
                     PSIDPYLWLLFPIVAVWGICFYSFNLYRPRRMGSHLAEFVDIAKANTLSILILVALTF
                     FSKPFEFSRLVIMYFWLLNLVVLGFSRMVFREILRVFRRMGYNQRQVVIIGAGKLGQR
                     VGDTLKMHPELGLQVRGYLTRNAEKVGQTLEGTPVIGTFDQAAEVLTSNVDIVFLCLP
                     PEVESEAEGLMKILSATTAGVKIIPSIY"
BASE COUNT         1353 a         1118 c         1453 g         1687 t
ORIGIN      
        1 ggattcgtct gatctgagat ttttggaagc tacacgtgct tttgtccgtc tggttctgga
       61 ggaaccaata gcttgttctt gctgcaattg ttattcgtaa agcagggggt gggtgccgaa
      121 ccggtttcct aaaagtattt catacgaaaa ttattggcag aattgaaacc tccccttccg
      181 gctcattggc gaaaagttag ccgtgcaatc cttaaaacga atgaaacaga actctgtcat
      241 tgttatggga tcaaccatta ttgctatttt tatcatggaa gctttactac gtgggattgg
      301 ggttccatct aaacttaact cgggttgggg atgggaaaat tctgcgggta gaaaattgtc
      361 aaaatatgat gctttgacaa ccaatcagtt tggatatcga ggacaaaaca ttaactatga
      421 tgccgatgac tatgtggtgc tgcttgtggg cgacagccag gtcgaagcgt atgcaggtcg
      481 gccggaacat atgcctgaac agtttcttca ggaattcctt actagtcagt tgcataaacc
      541 agtgaaggta ttttctcttg ctgcttcagg gtggggacag gaccaacagc tcctggccat
      601 tcaagaatat tttagggttt atagggcaga cttggtttta ttatgggcaa ccccgggaaa
      661 tgacttttgg gaaaatgcgt ttccagatag aagcgtgact ccgctagcgg gccctttgaa
      721 gccaaccttt aggttgatag atgggaaact ctctggccct tattttacat cagaatccta
      781 cttgcacaat agtgctatgg ctcaattaat tgagactgtg atggcaaata tgcaacagga
      841 aacattagaa caacgcattc ttcgtcgttg gcttcaaaag atgccatcgt cccatacaac
      901 ccataaaccc gataatgaaa atgtgtgtgg gggactgaca gttgttaatc aagtagagtt
      961 ttttaacacg atttttgatc tcaataagga tattggttat acggttaagt ctggacagga
     1021 tgtcgtgaat agcagaagtc tcttttctgc ttatatgtta gatccatcca atcgagatga
     1081 atatcttgtt gcgattactg aaagtctttt gtggcatgta aaaacggaag tgaaaaagaa
     1141 tcattcggaa tttcttgctt tttatcctgt tcgggaagat tttgataagc gaggaatgca
     1201 aatggtgaga tgtgtggcgg attttcaagg aaatatattt agggtttctt tggactttac
     1261 aagtcgcttg caagatgtaa tctcttctga agacttagtt gtctttgatt tgcttggtgg
     1321 taacgaaatt gttgtttcac cggaagatag gcatttaaat gatttcggaa acgaattagt
     1381 gatgaagaaa ctgtccttaa ctcttatgga gcgttcgata ttcaattgat aggaatggtt
     1441 cccctccggg gcatcaatgc atttatttcg atggggttgg attaggcatg caggcatttc
     1501 cgccagcgcg ctgacgaaat tccctggtcc aatgctatat gtaacccatg gaaaaggatg
     1561 ttatctttag tacatgtccc ccagggtgag tctgttagca ggcagctgtc taatagaccg
     1621 tatctatgta acctaatagg aaatctggat caatggaggg tattccgact acgcaaaagc
     1681 tctgttccat catcattgtg acctataatt cgagttcctg catcggtgcc tgtctgagtt
     1741 ccttactaaa catttccgat attgagctcg tggttgtaga taatgattca aaggatggta
     1801 ctgcagcaaa attgcagaaa gaatttccac agatcactct tattgccctt caaaataata
     1861 ttggttttgg gcgtgcgtgt aatatcgggt tggcggcttc gagtggctcc tttgccctat
     1921 ttttgaaccc ggacaccatt gcgaatgaaa aggccattcg gactcttgtc caattttatg
     1981 agaagcatcc gcgagttgga attatcggag gccgtcttgt tgatccgtgt ggcaagcctt
     2041 tgcaatcaat gggggatacg ccttctttga ctggccttgt gctggataag cccttggcct
     2101 gggtggctaa gcgtgtgggg gctcaagggg tctttcgtcg gatattaggc cgatgttcgg
     2161 caaaattttt aatgcctcct gaagcagagc ccgtggcctg ggtgtcgggt gcatttcttt
     2221 gttgccggcg ttcaatctgg gatacaattg gcggctttga tgaaaatttt tttttgtatt
     2281 atgaagatgt cgatctgtgc ctcagggcca agcaggctgg ttgggaggtg tggcacgttc
     2341 ctgaagcggt tgtagagcat cagtccgggg cttcatttgg aggcgatctt tttaaacaaa
     2401 aagaaattta ttatgctaat caatattatt tttttcggaa acattttggt cgtccgatcg
     2461 catgggcctt gtggattttg cagagtgtct attctcgatt aggaatgtac cgtcgccttg
     2521 gaaccgaccg aattggtcgt gggttgttat agtgttgatg gggagaaaca cagagagtct
     2581 gtatgagaaa ttggggccgt aatgcgggtt ctccaaattg ttgccgatgg taaaccaggg
     2641 ggaggcacaa cgcatgtgct tcagatcctc agaggtttac ggcatgccgt ctcttttcaa
     2701 ctcattacgg aagaacaatc atatttaatg aaggaagccg gtgctcttgg aattccctgc
     2761 caggggcttc gtttttttat tagccggctt aattttgccc ttcctttcca ccttcgtgcg
     2821 ctggttttgg cccttcgacc tgatctggtt catgtccatg gtgggcgtgc ggggtttttc
     2881 tttgcgttat ctctattgaa aatcccgatg gtatataccg tccatgggtt tcattttatt
     2941 gaaaagtccc cgagaattcg ccggatggca ttaatggcgg aacgttggaa ttttcgacgt
     3001 gcttgccatt ctattttcgt gtcgaagtat gatgtgactc ttgcagaaaa ttctcgatta
     3061 ctatcaggtt gtgacaaaag ggtcgtcatc tatcctggtt tttctttgaa aaatcttccg
     3121 aaggctcacc cgcgtacgct tctacatatt gggtttcttg gacgattgga atatcaaaag
     3181 gatcctctct tgtttttaga aatgatggcc tatctgcctg agtattcggc cacgattgtt
     3241 ggtgatgggg cgttggctcc gatgatcaag caggaaattg ccaagagggg tttatgcgga
     3301 cgggttcaca tggttgggga gttgtctcat ggagaggctc tggaaattct cgctactttc
     3361 agcgttgtgg ttttaacctc caggtgggaa ggcttgccgg ttttggtctt agaatctatg
     3421 ggtattgggg ttccggtcgt gagcatgaat gtgagtggat tggaagaaat cattcatcac
     3481 gaggtaaatg gaatgctggt agagaaaaga agaggggaag atcttgcgga taaagtcaga
     3541 ataattaccc acaatgaggg tctcagggct ttcctcatta agaatgcaca agcgactatt
     3601 cacgaggaat tttccatgga caatatgctg ggttcgatct tgaaaatata tcaagaagca
     3661 tctgttgcat agcggccggt tataaaggat acaagtgaag gttgcgctgg ttcacgattg
     3721 gctcactgga atgcgagggg gggagcggtg ccttgaagcg ttgtgtgaac tgtttcctga
     3781 tgcgccaatt tatacgttgt tccacgtcaa aggcagtgtg tcggatacga ttgagcggca
     3841 tcccattatt ccaagctttc taaatagaat cccgtttgcg aagagccgat atcggtattt
     3901 gctccctttc tttccttcag ccattcagcg gttagaaatt caacagtatg acttggtggt
     3961 gagttcgagc cactgcgtgg ccaagggtat tcgagtgcct cgcgagacct gccatatatc
     4021 ctatgttcat acccccatgc gatatatttg ggatggattt gatacctatt tcggaaacgc
     4081 aggattttgg aatgttggca ggttaggaat ggcccttttt cgggcgcgac ttcagcagtg
     4141 ggatatggaa tccaatgccc atgtgagtcg ttttattgcc aattcccaca acgtggcaga
     4201 acgaatcacg cgacagtatg ggagaccggc atacgtggtc tacccacccg tcgattggca
     4261 gggccatcag gtgtcgcgtt gccatgaagg atttttcttg atggtcacgg cctttgcgcc
     4321 ttacaaaaaa gttgacctgg ccattgccgc agcgaatgaa ttagagattc gattgaaaat
     4381 tattggccaa ggacaggatg aaaaacgatt aaagcgaatg gccggcccta ctgtcgagtt
     4441 tcttggctgg cagccagacc atcgtgtcag agacttttac agtcggtgtc tggctgttct
     4501 cttccccggt gaagaggatt ttggtattgt gccattggag gcaatggcca ccgggaaacc
     4561 ggtaattgcc tatggaaagg gtggagccct tgaaaccatc gttcctctca atcctttgcc
     4621 taagctaggc gaaaatcatt tagaacgggg cgctcaaacg agggaatctc attccgggct
     4681 gaccggggtc ttccactatg agcagtcggt ccaagcaatc atcgaagcca ttcagttatt
     4741 caaacagcac ctcactgagt tcgacccgga tgcgattcgg gaccatgttg aaccatttga
     4801 tcgctcgcat ttcaaagagc aggtgcagca agttatcatg tccgcttacc atgagtttcg
     4861 tcgggtccca ctatgttgaa acgtcacagt gagtttttaa aaaatcttct cttcctctcc
     4921 gatttggttg tgatctgcgt atgttgggtt ggcgcctact tcattcgttt ttctgtgccg
     4981 ctatttccca ttaccaaggg cattccttcc attgacccgt atctctggct gctttttcca
     5041 attgtcgccg tatggggaat ttgtttttat tcatttaatc tctatcggcc tagaagaatg
     5101 ggttctcacc tggcggaatt tgtcgacatt gccaaggcca atacgctgag tatcctgatc
     5161 ttggtcgcat tgaccttttt ctcgaagccg tttgagtttt cccgtttggt gatcatgtat
     5221 ttttggttgt tgaatttggt ggtattgggt ttttccagga tggtttttcg ggaaattctc
     5281 agggtattca ggcgtatggg atacaaccaa cgccaggtgg tgattatcgg agcaggaaaa
     5341 cttgggcaga gggtgggaga tacgctcaaa atgcatccag agcttggcct ccaggttcgc
     5401 gggtatttga ccaggaatgc tgaaaaggtt ggtcaaacgt tggaagggac acctgtcatt
     5461 ggaacatttg accaggcggc agaagtttta accagcaatg tggatatcgt atttttgtgc
     5521 ttgcctcctg aagtggaaag tgaagctgag ggactgatga aaattctgtc tgccaccaca
     5581 gcaggagtga agatcattcc atccatttat g
//
LOCUS       DAORAQ010000163         5603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2098_length_5603_cov_66.765501, whole genome shotgun sequence.
ACCESSION   DAORAQ010000163 DAORAQ010000000
VERSION     DAORAQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2098_length_5603_cov_66.765501"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKM72_11660"
     CDS             <1..545
                     /locus_tag="PKM72_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNP61489.1"
                     /translation="LLLLVTFSLDNEQAGHKVHALFGQVEWITIFFFCGLFIIVTGVE
                     HTGLLQSVADWTLSMTAGNFTVTALAILWVSAILSAIVDNIPFVATMIPMVKSMLPVF
                     QQAGIPLEQAETLWWALALGACLGGNGTLVGASANLIVAGIAERNGVPFRFLPFLKIA
                     FPLMILQVLISTLYIWMRYL"
     gene            560..991
                     /locus_tag="PKM72_11665"
     CDS             560..991
                     /locus_tag="PKM72_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP61490.1"
                     /translation="MIRQPHTIPPQLTIGEALRQMADLRLQDFPVMDEKKMLLGTLNF
                     WQILELAMPPYISQGDLPDVRFAPNLVRFHERLGELKPNPVTQVMNPHPPWARPDDSV
                     LACAALIMKTPKTVYLLPVVEGDRQLVGIISAWDLIKEIAG"
     gene            complement(1046..1504)
                     /locus_tag="PKM72_11670"
     CDS             complement(1046..1504)
                     /locus_tag="PKM72_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61491.1"
                     /translation="MKAKGKGRWGVWYTVICLAIAICVAGGGNAVSQEPASSTPRQVV
                     EQWVEVFPGNIELAAELTTTAFREGASKEEWIATRGPYLRNLQLKYVRTNIAHEKMVG
                     DEAHVIVHAHIVTVMGDQPQDELYVLLKNPEGRWVIDQVEVYTETFNSSP"
     gene            1836..2699
                     /locus_tag="PKM72_11675"
     CDS             1836..2699
                     /locus_tag="PKM72_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61492.1"
                     /translation="MRVLLATDGSEQSFHAAKAVMSLASVSTLTILHVIDLPRLTFSM
                     LGPEIAYDLSKVAEEALRKDGEQALTHTNALLSDKVKHVEIRLEEGSPAEHILSVSQE
                     EHPDLILMGARGRGQVEELFLGSVSQRVLTHAACPVLIINGPLTEIKKILIAIQSPDD
                     VQKVGQFLTKHPFQPQTEITLLSVVPIPRSLFRGGVSAPEEKIEQALKSTEDFLDQAV
                     SQLKNSYDSVKGYVGLGAPANTILEQASITEPDLLAMGMHHPSTITRFVLGTVSHTVL
                     HQATRSVLVIR"
     gene            3075..3899
                     /locus_tag="PKM72_11680"
     CDS             3075..3899
                     /locus_tag="PKM72_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61493.1"
                     /translation="MKTTAQANGWINGQRSNGISLADRLRSGYHRFWYEASQAMRWSW
                     GPFRESSKGPLPNLTQTQQQRTDELARSYRSRFENSLPPETALLNYSYLDILDRAKHA
                     LGWRVPTNQRVCDLGSANFAYAAALHTFFHPNRLAGVEIDGHRLYCNGRSRIDYARGH
                     IQDLPQTEYVIADYRRFTRQAEIITAWYPFVTPKPLLAWRLPLSLFHPAAVFAQVANN
                     LVIGGTFIMVNHSAAEAEVARGIAEDVGLVCDGHYLHKAHLRPRIADPVLTIWHHG"
     gene            4144..4707
                     /locus_tag="PKM72_11685"
     CDS             4144..4707
                     /locus_tag="PKM72_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61494.1"
                     /translation="MQNGKEGNRSLQNQQPLAWGISPLLIVGTFYVDLHTPLGVACAI
                     PYILAVLIASFLHPAWPMPMIALVSSILTIVGIFYSPEPLVVPFWKVLTNRGLALFAI
                     WVIAILLYHRNALIARKEEALARIQLLENLLPICAECKKIRDQRDTWHQLETYLTAHS
                     NLTFTHGICPECESQALANFKREQFVQ"
     gene            complement(5058..5516)
                     /locus_tag="PKM72_11690"
     CDS             complement(5058..5516)
                     /locus_tag="PKM72_11690"
                     /inference="COORDINATES: protein motif:HMM:NF027715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5008 domain-containing protein"
                     /protein_id="HNP61495.1"
                     /translation="MLVRISCIFGPLFIFAIGLIGSVSFWGQGASAQAVEAPVDFVIP
                     ESIKGFDEEKVKKDPICDSSVRPQMDHVKPDEMKPGDTVVVEGKSFGKKKECLFGVTF
                     GAEPAKAFTLVDDEHIQVVVPEGLRSGVTFLNIETGGGTARKGVLVRSKD"
BASE COUNT         1437 a         1511 c         1250 g         1405 t
ORIGIN      
        1 tgctcctgtt actcgtcaca ttttccttag acaacgaaca ggcagggcat aaagtccatg
       61 cattattcgg gcaggtggaa tggattacca ttttcttttt ctgtggccta ttcattatcg
      121 tcaccggcgt ggaacatacc ggccttttac aatccgtggc agattggacg ttgagtatga
      181 ccgctggaaa tttcaccgtg acggcattgg ccattctttg ggtttcggca atactttccg
      241 ctattgtgga taatattccc tttgtcgcca cgatgatccc catggtcaaa agcatgctcc
      301 cggtctttca gcaagctggg attccgcttg aacaggccga gaccctctgg tgggcattag
      361 cgttgggcgc ttgcttagga ggcaacggga cgctggtagg ggcctcggcg aatttgattg
      421 tagcggggat tgcggaacga aatggcgtac catttcgatt tttacccttc ttgaaaatcg
      481 cttttccgct catgatccta caagtgctga tcagtaccct ctacatttgg atgaggtatt
      541 tatgaacgcc agggacatca tgatccgcca gccacatacc atccctccac aattgacgat
      601 cggggaagcc cttcgccaaa tggcggatct gcgcctccag gactttccgg tcatggatga
      661 gaaaaagatg ttactgggaa cattgaattt ctggcagatt cttgaattgg ccatgcctcc
      721 ctacatttcc caaggggatc ttcccgatgt tcgttttgct ccgaaccttg tacgattcca
      781 tgaacgcctt ggagaactta aacccaaccc cgtcactcag gtgatgaacc cccatcctcc
      841 atgggcccgg ccggatgact ccgtactggc ctgcgcggct ttgatcatga aaactccgaa
      901 aacggtctat ttactgcccg tggtggaagg agatcgtcaa ctggtaggaa ttatttctgc
      961 ctgggatctc ataaaagaaa tcgcagggta gaaaacaagg cgggacatac ctgcatcatt
     1021 tgatttggtt ctaactcctt ccgtttcagg gactcgaatt aaacgtttcc gtatacacct
     1081 ccacttgatc gatcacccac ctgccctcag gattcttcag caggacgtac aactcatcct
     1141 gcggctgatc ccccatcacc gtgacaatgt gagcatgaac gattacatgg gcttcatctc
     1201 cgaccatctt ttcgtgggct atgttggtgc gcacatattt taactgtaag ttccgaaggt
     1261 aggggcctcg ggtggcaatc cattcttctt ttgacgcacc ttcccggaat gcggttgtag
     1321 tcagctccgc agccagttca atgttccccg ggaatacttc aacccactgc tccaccacct
     1381 ggcggggtgt ggaggaagca ggctcctggg aaaccgcgtt ccccccaccc gccacacaga
     1441 tggcaatcgc taagcagatc actgtatacc atacccccca tcgccctttt ccttttgcct
     1501 tcatgttaaa atcccattca tttgacttag atctgatacc tcaataggtc cctgctcttt
     1561 caatatacta tacgagtaag gtccagggga aaacataaag gttccatggt cattaccacg
     1621 ccttccacag atctcattgt tggaatcgag agtgccacga aatttggtaa ataaaagcat
     1681 ggggtcttct cgacatggta ccgttcacag atgcgcctgg agtcacccag agaaaaaggc
     1741 tcatccccaa gaacacacac gccccataca cagactgtgt gagaagacag gaccatgcaa
     1801 gatcagcata aaaattacaa gaggagaatt aacacatgcg agtactccta gccactgatg
     1861 gatcggaaca atcctttcac gccgcaaagg cagtcatgag tttggcctca gttagcacac
     1921 tgactatttt gcacgtcatt gatctccccc gattaacctt ttcaatgctc gggcctgaaa
     1981 ttgcctatga cctctcaaaa gtcgcagaag aggcactccg caaagacggc gaacaggctt
     2041 taacacatac aaacgctctc ctctcagaca aggtgaaaca tgtggaaatc cgattggaag
     2101 aaggttcgcc ggccgagcat attttgtcgg tgtcccaaga ggaacatcct gatcttattc
     2161 tcatgggcgc ccggggaaga ggacaggtcg aggaactttt tctgggaagt gtctcgcaac
     2221 gggttctgac ccatgcagca tgtccagtcc tgatcatcaa cgggccatta acggaaatca
     2281 agaaaattct gattgccatc caaagccccg atgatgttca gaaggttgga cagtttttaa
     2341 cgaaacaccc ctttcaacca cagacagaga ttaccctctt gtcggtagta cctattcctc
     2401 gttccctctt tcgaggcggc gtctctgcac cggaggaaaa aattgaacag gccctgaaga
     2461 gcacggaaga tttcctcgac caagctgtca gtcaactcaa gaactcttac gattcagtta
     2521 aggggtatgt agggttagga gccccggcga atacgatatt ggaacaggca tcaatcacgg
     2581 aaccggatct cctggcgatg ggaatgcacc atccttcgac cattacacgg tttgtgttgg
     2641 gcaccgtgtc acataccgta ctccaccagg ccacgcgatc cgttctcgtc attcgataac
     2701 aagatatgct gccatcggac taagagttct ttggtttcga ctcctggatg gtaggtggaa
     2761 tagacataca cgaaatcgag aaatcccggt tgcttttcaa taagcaactt gacatccttc
     2821 caatacaatc tcgcactcct gtcataaggt ttagtagaac cacactgctg aacttctcag
     2881 attcaatagt cttgcttcca taagtcaggt cccatggtcc ctttgtccta caaaagaaaa
     2941 actaccattg tgcgagacgc caaaggatcc atgctgacaa ttctcgcatg accatttaag
     3001 accactctcc cctctaccga aaaacataaa agagcgatca agatatcagt tcatcttcaa
     3061 caggtacccc agaagtgaaa actactgcac aagccaacgg ttggattaat ggccaacgct
     3121 caaacgggat atccctggca gaccgcctac gatcgggcta tcaccgattc tggtatgaag
     3181 cctcacaagc catgcgatgg tcatggggac cattccgcga aagctccaag gggccactgc
     3241 ccaatcttac ccagacccaa caacaaagaa ctgatgaact ggcgaggtca tatcgttcgc
     3301 gcttcgagaa ttcccttcct cctgaaactg ctcttctgaa ttattcatat ctggacatat
     3361 tggatcgtgc caaacatgct cttgggtggc gggttcccac caaccaacgg gtctgtgatc
     3421 tcggaagtgc caatttcgcg tatgctgcgg ctctacacac cttctttcac ccaaaccgcc
     3481 tcgcaggcgt ggagatagat ggccatcgtc tgtattgtaa cgggcgaagc cgcatcgatt
     3541 acgcccgcgg gcatattcaa gatttgccgc agaccgagta tgtgatcgcc gactatcgcc
     3601 ggttcaccag acaagccgaa atcattacag cctggtatcc gttcgtcact ccgaagccct
     3661 tactggcctg gcgattaccg ctcagcctat tccatccagc cgctgtattc gcccaagtgg
     3721 ccaataatct cgtgattggc ggtaccttca ttatggtcaa tcacagtgcc gccgaagcag
     3781 aggtcgcgcg aggcattgcg gaagacgtcg gtctcgtttg tgacggccac taccttcaca
     3841 aagctcatct tcgcccgcgc atagcagacc cggtgctaac catctggcat catgggtaat
     3901 tggaattagc tggctagtca ttgcttgaca cagggaactc ccttgggact cgatctcacg
     3961 attctcaatc tcttgttgcc cttacgagac aaaatctcca aagtctctgc taagactcgc
     4021 cccctgatgg aaactatcaa aacgtccttc actcatttat aatgccgcga aatttgctgg
     4081 ctgcccaatg ccggatctca gaaaggcatt tcgattctca cgttccccac ttggagaaaa
     4141 tccttgcaga acgggaagga aggaaaccgc agcctccaaa atcaacaacc gttagcttgg
     4201 gggatttccc ctctcttaat tgtcgggaca ttctatgtag accttcacac accattagga
     4261 gttgcttgtg ccatccccta tatcttagcc gttttgatcg cgtcgttcct gcatcctgca
     4321 tggcccatgc ccatgatagc ccttgtcagt tctatcctaa ccattgtcgg gattttctac
     4381 tcacccgaac ctctggttgt cccattttgg aaggttttaa cgaatcgggg attagctctt
     4441 tttgcgatat gggtcattgc aatactgttg tatcatcgca atgcgctcat cgcccgaaaa
     4501 gaggaagctc tcgcgcgaat tcaattgctg gaaaaccttt taccgatctg cgcggaatgc
     4561 aaaaaaattc gtgatcagag ggacacctgg catcaactgg aaacgtacct taccgctcac
     4621 tccaatttga cctttacaca tggcatatgc ccggaatgtg agagccaagc cttggcgaat
     4681 tttaaacgcg agcaatttgt gcaataaata ttcttatctt tttaagaata gaatatgggg
     4741 acgggacaaa aggaactacc cgcgcggcgt tccggggaaa acccttcggt ctcccctgca
     4801 agatccatta cggattcgct caagtgggag gagcaagcac gtgctgaacc ggtataggtt
     4861 agcggttaaa taggctcaca ggccagtcga aaccgattgg atccagtcaa ccctattgca
     4921 gatgagcgaa gatgcggata gacacaaact ttttattgga agcgagaaat gagaacagtc
     4981 cttaaatacc tcaagactga aaaacccgga atgggttgaa atgtaaaaga gttatttccg
     5041 tcgattacgg aatgcagtta atctttggag cgaaccagaa ccccctttcg tgccgttcca
     5101 ccacccgtct cgatatttag aaatgtaact cccgatcgta acccttccgg cacgaccact
     5161 tgtatgtgtt catcatccac taacgtaaat gccttcgccg gttctgcccc gaaagtcaca
     5221 ccgaaaaggc attctttttt cttcccgaaa ctcttgcctt ccaccacaac ggtatcacca
     5281 ggtttcattt cgtccggttt cacatgatcc atttgaggac ggacgctgga atcacagatg
     5341 ggatcttttt tgaccttttc ctcatcaaac cccttaatcg attccggaat cacaaaatca
     5401 actggcgctt ccactgcttg agccgatgct ccctgccccc agaaactcac cgacccgatc
     5461 aggccaatgg caaaaataaa aagtggtcca aatatgcagg aaatcctaac caacatgagc
     5521 aacctcccaa gttaaattgt taggaagatc gtttttctat actcttcagt caaggcaaca
     5581 tggatcgatt catgggtctt att
//
LOCUS       DAORAQ010000164         5500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2144_length_5500_cov_63.270707, whole genome shotgun sequence.
ACCESSION   DAORAQ010000164 DAORAQ010000000
VERSION     DAORAQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2144_length_5500_cov_63.270707"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            569..1129
                     /locus_tag="PKM72_11695"
     CDS             569..1129
                     /locus_tag="PKM72_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61496.1"
                     /translation="MPVNALRKSGARMSLQEALARVEKLKRLSGSSNPHEAALAAMRL
                     KDFNVEAARYPRSEPVIENVPPPDDQAYLNKVVEILDEMPIVPYETLDEIEVGQSPDK
                     SKVNWDELHFALVKKAQRMGADALIHIQLKGTAEKKVLGATALKYLTPKEVQEIQQST
                     ALDDAEKAYNESQKERLDEDLAPGIG"
     gene            1484..2161
                     /locus_tag="PKM72_11700"
     CDS             1484..2161
                     /locus_tag="PKM72_11700"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNP61497.1"
                     /translation="MRYTNQDLSILPANGDVMSCYHNTTKGPTVERRKIESKVPATIT
                     GVLVMTVCLTVIFSGLAHTKETEVLSVGIRGGINSETLGIPPTEKEDFEQYDVFAVVG
                     TPWSWDFSSGWDIRFRLNVSAGTLRAAGDSGFIGEVGPGLAFSNPSWRFTFDIGGGAA
                     YLSEEKYGRQDIGGPVQIIGHGGITYHLPWRISAGYRFHHMSDAAIYGSNNRGVDLHM
                     LELSYRF"
     gene            complement(2316..3710)
                     /gene="sthA"
                     /locus_tag="PKM72_11705"
     CDS             complement(2316..3710)
                     /gene="sthA"
                     /locus_tag="PKM72_11705"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNP61498.1"
                     /translation="MTSPTPFDLIVIGSGPAGQKAAIQGAKAGKQVALIEQTREVGGA
                     CVYQGTIPSKTLRESALQMVRFLQATEVFEFRMRDDVKITNLMTRLDRVVRAHGKFMK
                     DQLTRNGIDCFHGRAGFVSAHEVRIQKTDGTATLVTAPIIVVATGSRPRVPENIPIDH
                     EHILDSDSILSLVYLPKSLTVLGGGVIASEYASIFALLGVHVTMVDTADRPLKFLDQE
                     LTDEFCEAFEKTGGHYRGGQKIATVQWDGCSKVTTVLESGETLESEKVLVALGRVSNL
                     EHLHLQATGIQPTTRGLIPVNHHCQTTVPHIYAVGDVIGPPSLASCSMEQGRRAVCHA
                     LGLNPGVAPEHIPMGIYTIPEMSSVGLSEQEATAKYGGATVGRARFHEIARGQISGMT
                     HGLLKMIADPKGEQLLGIHIVGEGATELIHIGQMGLLHNMPIDRFIDNIFNFPTLAET
                     YRVAALDIAKQRQH"
     gene            complement(3707..4582)
                     /locus_tag="PKM72_11710"
     CDS             complement(3707..4582)
                     /locus_tag="PKM72_11710"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNP61499.1"
                     /translation="MAHLTHPSLAQEEKETLRSFIADQLERGNLDLPLLPVVANRVLL
                     LSNDPNADASQLSSLIQHDQALAGQILRIANSPAYASRTPIVSLQQAITWLGLTMLAG
                     LAFSVSVQSGVFHTTGYEQEVRNLWRHALATGLFGKEIARRIRHNVENAFLCGLLHTI
                     GKPAILHLLSQSPEKSSEPVSWITVESLFEEFHGMAGSKLAAVWQLPDPVQETIRYYQ
                     DDQYHQAISPTKGAMITCLAHHLASDMLGNPRHDENSLLTLPVVQDLNFYPEDMTALL
                     ELRETVKASVECFLP"
     gene            4866..>5500
                     /locus_tag="PKM72_11715"
     CDS             4866..>5500
                     /locus_tag="PKM72_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP61500.1"
                     /translation="MKRLEGKIAVVTGSSSGIGKAIALRFGEEGATVVVAARRVDLCH
                     QTVAQITEAGGSAYAQQTDISQEAQVDALMQETIARYGRIDILVNNAGLVGGGRVADT
                     STKMFDEVVGTNLRGTFFCCRAGFQHMKRQGGGLIINISSVAGIQAWAGTGIYSASKH
                     GIMALTKSLADEGRAFHIKVSAICPGGVADELVDATPEEILDSEKIDPYDVA"
BASE COUNT         1363 a         1259 c         1543 g         1335 t
ORIGIN      
        1 aaatagacag atcagaatcc aagaggaaca cggttgcgcg aatgaatgag gaataataag
       61 tcgttattgc ttccatgacc gagaacgagt ccggctaaac taatcggaat aaagctgttc
      121 gcgctgatcc cgtgttcatt ccacattttc cgaccaatca gagtctcaca ggggccttaa
      181 atctcaattt tagttaggga agcttctggg gtcaaagaca ttatcctggc tcctaaggtt
      241 ggaatatgag ttcttggtct attagccaaa gtccttgaag gtcgtcggtg gcccaaggtc
      301 aacttgtggt ttccatggcc attggccgtt cgccagccta aggaagggga aggactttgg
      361 ggatggggag tcttattgtc ctttccaaaa gaccatttga caacctgaat cctgggaagg
      421 tagaaaaaac agcaaaagct taggtccatc tgctatttaa cggcttcttc ttgtattcaa
      481 aacctaagga cggttgatta tgaaaatttt cttacctttt taaaatgagc cacggaagac
      541 ctatggcaag cgatcatgga gttgtgaaat gccagtgaat gctctgcgga aaagtggggc
      601 cagaatgagc ctgcaagaag ctcttgcgcg agtagagaaa ctcaagcggt tatcaggaag
      661 cagtaatcct cacgaggcgg cattggccgc catgcgactg aaggatttta atgtggaggc
      721 ggcccgatac cctcgttctg aacctgttat tgaaaatgtc cctccgcccg atgaccaggc
      781 ttacctcaat aaagttgttg aaatacttga tgaaatgccc attgtcccct atgaaacgtt
      841 ggatgagata gaggtcggac aatctcctga taaatcgaaa gtgaattggg atgaactcca
      901 ttttgctctg gtgaagaagg cgcaacgtat gggggcagat gccttgattc atattcaatt
      961 aaagggtacc gctgagaaaa aggtgcttgg cgccacggca ttaaaatatc ttactccaaa
     1021 agaggtccaa gaaattcaac aatccacggc gttagacgat gctgagaaag cctataacga
     1081 gtcccaaaaa gagaggcttg atgaagacct ggcccctggt attggctaac taacacatgt
     1141 ttggtccgag tgaagaaagt ctgttaggcg tgaatggagg aaacgagaaa gggccgtcac
     1201 cttcgaattc gggatagacg gcctcaaaaa atcgaaaagt tcgttgaccg tgttgacttc
     1261 cttcccctga gtctcaaagg ggggacgaca ggcgagtgag aatatgatag aatcgcctca
     1321 tcgtctaccc cctcattaca ccacaccatt gtcattattt ttgtaatccc ctcttataat
     1381 tttttctcac agacctcgtt cagggggcag agaagtttca cgcgtggaaa gatcgtgaat
     1441 aacctctctc tggcctcaag tggcgatgtg tcaagggacg agcatgcggt acacaaatca
     1501 agacttgtcc attttgccgg cgaatggtga tgtgatgagc tgttatcata atacaacaaa
     1561 aggaccaact gtggagagaa ggaaaatcga gtccaaagtc ccagcaacga taaccggtgt
     1621 cttggtgatg acggtgtgtc tcacggtaat tttttcgggc ttggcccaca caaaagagac
     1681 ggaagtactc tccgttggga tacgaggagg gatcaattct gaaacgttag gcattccacc
     1741 caccgaaaaa gaagattttg agcaatacga cgtgtttgcc gtggtcggaa ctccttggag
     1801 ttgggatttt tcctccggat gggatatccg tttcaggctg aatgtttctg ccggaacgtt
     1861 gagagcggca ggagactcag ggtttatcgg ggaggtcggc cctgggcttg cgttttcgaa
     1921 cccaagttgg agattcacct tcgatattgg cggtggcgcg gcgtatctga gcgaggaaaa
     1981 atatggccgc caggatattg gcggacccgt tcaaattatc gggcatggtg gcattaccta
     2041 tcatcttccc tggaggatat ccgccggata tcggtttcat catatgtcag atgccgcaat
     2101 atacgggagc aataatcgcg gagtggatct gcacatgctt gaactcagtt accggttctg
     2161 aggatgtttg agaagatttc tgccattatg gggtagcgca ccgatagggc cttccccctt
     2221 caacctgtgt atctatccat ggccgaatca ttcatcatcc aatgatgacg ggttcgatgc
     2281 tgggaacatt ccatccaaat acagaagcca gttgttcagt gttggcgttg tttggcgatg
     2341 tcgagtgcgg cgacccggta ggtttcggcg agggtcggga aattgaagat gttgtcaata
     2401 aatcggtcga tgggcatatt gtggagcaag cccatttggc cgatatgaat cagttcggtg
     2461 gctccctctc ccacgatatg aatgcctaac agttgttcgc ctttcgggtc ggcgatcatt
     2521 ttgagcaatc catgggtcat gccggagatc tgtccacgcg caatttcatg aaacctggct
     2581 cttcccacgg tcgctccgcc atacttggcg gtggcttcct gttcgcttaa tcccacgctg
     2641 gacatttcag gaatggtata gattcccatg gggatatgct caggagcgac gccggggttg
     2701 agacctaagg catgacagac agcacggcgg ccctgttcca tggagcagga tgccagcgac
     2761 ggggggccga tgacgtcacc gactgcatag atatggggta ccgtagtttg gcagtggtgg
     2821 ttgaccggaa tgagcccacg agtcgtcggc tgtatcccgg tggcttgtaa atgtaaatgt
     2881 tccagattgg acactcgtcc gagagccacc agcacctttt cactttccag cgtttcacca
     2941 ctttctaaaa cggtcgtcac cttagagcat ccgtcccact ggacggtggc gattttttgt
     3001 ccgccccgat agtgccctcc ggttttctca aacgcttcac aaaattcatc ggtgagttct
     3061 tgatccaaaa atttgagtgg gcggtccgcc gtatccacca tggtgacgtg cactcccagt
     3121 aaagcaaaaa tggaggcgta ttcactggca atcacaccac cccccaacac agtcagggat
     3181 ttcggcaaat agacgaggga gagaatggag tcgctgtcca gaatatgttc atggtcgatg
     3241 ggaatattct caggtacgcg tgggcgagag ccggtggcga cgacgataat cggtgccgtg
     3301 acgagagtgg ccgtgccgtc agtcttttgg atccgcactt catgggcgga cacaaagcca
     3361 gcgcgaccat gaaagcagtc aataccatta cgggtgagtt ggtccttcat aaacttcccg
     3421 tgggcgcgca ccacccggtc caagcgggtc atcagattgg tgattttgac gtcgtcccgc
     3481 atgcggaatt caaagacttc agttgcctgg agaaatcgca ccatctgaag ggcgctttcc
     3541 cgtaacgttt tactgggtat ggtgccctga tagacgcacg ccccgcccac ctcgcgggtt
     3601 tgctcgatga gggccacctg ctttccggct ttggcgcctt gaatcgcggc tttttgtcct
     3661 gccgggccgc ttccgatcac aatcagatca aatggggtcg gtgacgtcat ggcaaaaaac
     3721 actccacact tgccttgacg gtttcacgca attccagtag ggccgtcata tcttcagggt
     3781 aaaaattcag atcctgcacg acgggaagcg tgagcaggct attttcgtca tgcctgggat
     3841 tcccaagcat atcagaggct aaatgatgag ctaggcaggt gatcatggct cccttagtgg
     3901 gggaaatggc ttggtgatac tggtcgtcct ggtagtagcg gatggtttcc tgaactggat
     3961 cgggtagttg ccagacagcg gccaattttg aacctgccat accatgaaac tcctcgaaga
     4021 gggattcaac cgtgatccag gaaaccggtt ccgatgattt ctcgggggat tgtgagagaa
     4081 gatgaagaat ggcgggtttc ccgatggtat gcaggagtcc gcacaaaaag gcgttttcca
     4141 cattatgacg gatgcgtctg gcgatttctt ttccgaaaag ccctgtggcc aaggcgtgcc
     4201 gccagaggtt ccgtacttct tgttcatagc cggtcgtatg aaagaccccg ctttgaaccg
     4261 acaccgaaaa tgctaacccg gccaacatcg ttaaccccaa ccatgtgatg gcttgttgaa
     4321 gcgagacgat aggggtgcga gaagcgtagg caggggaatt ggcaatgcgg aggatctgtc
     4381 cggctagtgc ttggtcatgt tgaatcaatg aagatagctg tgaggcgtcg gcattcggat
     4441 cgtttgaaag cagaagaacc cgattcgcca ccacgggcaa cagagggaga tccagattcc
     4501 ctcgttccaa ttgatcggcg ataaaggaac gaagggtttc cttctcttcc tgtgcaagag
     4561 aggggtgtgt taagtgagcc atgtctcaac ttgtcggttt tcaggaaaca acctttagaa
     4621 ttgtctagta atgcccggta ttggattaaa aaatccggct tactttgagt ggtctccagc
     4681 tcaatctctc ggagaggttc ctctatttct gtggagacct ggatgagatc tcattcttac
     4741 cgtcagggta tggttcatca tggttggcct ggactggcgg ggtggcctac agtcactcca
     4801 agagtcggtc tggcgaggaa gacggctaac gtgttttgag gagagtccta aaggaggaga
     4861 acgttatgaa acggctagaa ggtaaaatcg cggtggtcac gggaagcagc agcgggattg
     4921 gcaaagccat tgcccttcga ttcggagaag agggggcaac ggtggtcgtc gctgccagac
     4981 gggtggattt gtgtcatcaa acggtggcgc aaattacaga ggctggcggg agcgcctacg
     5041 ctcaacaaac cgatattagt caggaagcac aagttgacgc gctgatgcag gagacgattg
     5101 cccgctatgg gcgaatcgat attctggtga ataatgccgg gctggttgga ggaggacggg
     5161 tggctgacac atccacaaaa atgtttgatg aggtggtggg gaccaatttg cgggggacct
     5221 ttttctgttg tcgagccgga tttcaacaca tgaaaaggca aggaggcggg ctcatcatta
     5281 atatctccag tgtagccggg attcaggcct gggcaggaac aggcatctac agtgcctcaa
     5341 agcatggcat catggccttg accaagtcgc tggctgatga agggcgagca tttcatatca
     5401 aagtgagtgc gatctgccct ggtggtgtgg cagatgaatt ggtggatgcg acgcctgaag
     5461 aaatcctcga tagtgaaaag attgatccct atgatgtcgc
//
LOCUS       DAORAQ010000165         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2190_length_5397_cov_75.769936, whole genome shotgun sequence.
ACCESSION   DAORAQ010000165 DAORAQ010000000
VERSION     DAORAQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2190_length_5397_cov_75.769936"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /gene="thrS"
                     /locus_tag="PKM72_11720"
     CDS             <1..1511
                     /gene="thrS"
                     /locus_tag="PKM72_11720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNP61501.1"
                     /translation="AIEFFKSRGEPYKVELIQGFPDGEPVSAYTQGDFVDLCRGPHLP
                     ATGYVKAFKLLNSAGAYWRGDERNPMLQRLYGTSFPSKEALQAHLDNLEEIKRRDHRK
                     LGKDLDLFSIQDETGPGLILWHPKGAQIRLLIENFWREQHLANEYELVYSPHVARLDL
                     WKTSGHVDFYKENMFATMSVESSEYQLKPMNCPFHIMVYKSHLRSYRDLPIRYGELGT
                     VYRYERSGVLHGLMRVRGFTQDDAHLFCRPDQLGEEVEKVLKFITSMLGTFGFTEFKM
                     FLSTRPEKAVGTIDQWDQATLALETALKNGGYTYQEDPGEGVFYGPKIDVKIQDALGR
                     SWQCSTVQVDFNNPERFGLTYVGDDGKTHQPIMIHRALLGSIERFFGILLEHFGGAFP
                     AWLAPVQVIVLPISEKHQGYAQEVEKNLRREGCRVSIDLRNEKIGLKIREAEKAKIPF
                     MIVVGDREAEAKMIAVRQRNGKNLGTMPIIDMVTLIREDMPESVKKGSLLFE"
     gene            1508..2026
                     /gene="infC"
                     /locus_tag="PKM72_11725"
     CDS             1508..2026
                     /gene="infC"
                     /locus_tag="PKM72_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNP61502.1"
                     /translation="MTRPVTPKQRINHFIKNPEVRVIGAEGEQLGILKTSEAIRQARE
                     IGYDLVEVAPTADPPVCRIMDYGKFKYEQSKKEHRMRLNQKSTQVKEVKFRPRTDKHD
                     METKVRQIRDFLEEGNKTKVTVMFRGREMANQELGFKAIQKVIEELKGAGNIESPPRM
                     EGRNLYMVVAPK"
     gene            2082..2285
                     /gene="rpmI"
                     /locus_tag="PKM72_11730"
     CDS             2082..2285
                     /gene="rpmI"
                     /locus_tag="PKM72_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNP61503.1"
                     /translation="MAKMKLKNHSGASKRFKRSGSGKWMRRKAGMRHILTSKSPGVKS
                     RLSGAAEVRKEDRTSLSRLLPYK"
     gene            2313..2681
                     /gene="rplT"
                     /locus_tag="PKM72_11735"
     CDS             2313..2681
                     /gene="rplT"
                     /locus_tag="PKM72_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNP61504.1"
                     /translation="MPRVKGGPQTRQRRKKRLKLASGQYGGKSKLFRTATESVDKGQA
                     YAYTGRKQRKRNFRQLWVTRISAAVRAQGVTYSQFMNALKKANVLLNRKMLSEMAIHD
                     PQGFSHLVSSTKGDGQPVPA"
     gene            complement(2678..4372)
                     /gene="pheT"
                     /locus_tag="PKM72_11740"
     CDS             complement(2678..4372)
                     /gene="pheT"
                     /locus_tag="PKM72_11740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNP61505.1"
                     /translation="MPTISIFQKDFCRLVGRNASMSDIEQWMPYVKGEVKDVSQDTGE
                     VRVELQDTNRPDLWCVEGIARQIRSVLNKGMPPYSFFSDKKGAKRRIQVAQGMETVRP
                     YVAACVSLGYAMTAEGLDQCIQTQEKLADAYGRKRETVSIGLYRYSSISFPVTYGLVK
                     PDEIRFTPLGFEEKMTPHEILTVHPKGLEYGSILAECDRLPLLWDSDGQVLSFPPIIN
                     SRELGEVQQGDTDLFVEVTGTDLSMVVLALNIFACNLADRGATIESVNITYPYETEFG
                     TTVKTPLSMNQSQRISLEAIEQALGMPLGSEAIQEALASYGYLVKATRQTVSVTLPAF
                     RNDFMHVMDVAEDVAITRGYDSFSPIMPQSFTVGSLSVEEQTSDRVRDLLVGFGFQEI
                     FSNIMASHQELVDRMRIADTEYAQLVEVDNLMSQTYACLRPSILPCLLRVEALSPRVF
                     YPHLLFEVGETAQLDLEANAGSRTTLSIAAISANSGANFSEIHSYLDLLMYYMVWPYE
                     LEPVTHPSFLEGRVGRIRCNGHSVGYIGEFHPEVLEAWQIDMPTSGFEFEIRKPEA"
     gene            complement(4382..>5397)
                     /locus_tag="PKM72_11745"
     CDS             complement(4382..>5397)
                     /locus_tag="PKM72_11745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNP61506.1"
                     /translation="KRGNTQEPFRIDDHTERTYVLTAEGRTLLPHLREGAAQEISQLT
                     PELLKDGSWRNVSFRKYSINLRPPRLAVGRRHSYREFLDTVKYKLTSMGFQEMRGELI
                     ETEFWDMDALFMPQFHPARAIHDVYFVKNPKMAKNIPEPFLSQVAQVHRDGGTSGSKG
                     WKYEYDSERAKRLVLRSQGTAVSARTLAQNPPIPGKFFSIARCFRYDNVDATHATDFF
                     QVEGIVLGSDINFRTLLGLLQLFATEMAQAKEIRFFPAYFPFTEPSVELHVRHPKLGW
                     MELGGAGLFRSEVTIPLGVTVPVIAWGLGLDRMAMVALGIQDIRDLFTSDLEAVRNMR
                     RQF"
BASE COUNT         1463 a         1238 c         1401 g         1295 t
ORIGIN      
        1 aggcgataga atttttcaaa agtcgtggtg aaccctataa agtagaactc attcaggggt
       61 ttcctgatgg agagccggtg tccgcttata ctcaagggga tttcgtcgat ctctgccgtg
      121 gcccccatct gccagccact ggctatgtca aagcattcaa actcctgaat agtgcaggag
      181 cctattggcg gggggacgaa cgaaatccca tgctgcaacg gctctatggc acctcctttc
      241 cttcaaaaga agccttacag gctcatttgg acaatttaga ggagatcaag cgtcgggacc
      301 atagaaagct tgggaaagac ctcgatttat ttagcatcca ggacgaaacc ggtccaggtc
      361 ttattttatg gcatcccaaa ggagcacaga tccgcctgct gattgaaaat ttttggcggg
      421 aacaacattt ggccaacgag tatgagttgg tctattcgcc acatgttgct cggttggatt
      481 tatggaaaac cagcgggcat gtggatttct acaaagaaaa tatgttcgcc accatgtctg
      541 tcgagtccag tgaatatcag ttaaagccta tgaattgccc gtttcatatc atggtgtata
      601 aatcacattt gcgcagttat cgcgatctgc ctatccgtta cggggaattg ggaacggtct
      661 accggtacga acgatccgga gtcctccatg gcctgatgag agttcggggc tttacccagg
      721 atgatgctca tttattctgc cgccctgacc agctgggtga agaggtggaa aaagtcctga
      781 aatttattac gagtatgctt ggaacttttg gatttacgga attcaagatg tttctctcca
      841 ctcggcccga aaaagctgtg gggacgatcg accaatggga ccaagcgacg ttagcgctgg
      901 aaactgcgct gaaaaacggc ggctatacct accaggaaga tcctggggaa ggggtctttt
      961 acgggcctaa aatagatgtc aaaattcagg acgctttagg ccgatcctgg caatgttcga
     1021 ccgttcaggt cgatttcaat aatcccgaga ggtttggtct aacttacgtg ggtgatgatg
     1081 gaaaaactca tcaacccatt atgattcacc gggccttact cggatcgatt gagcgatttt
     1141 ttgggattct attggaacat tttgggggtg cctttccggc ctggttggcc cctgttcaag
     1201 ttatagtcct cccaatttca gaaaaacatc aagggtatgc ccaagaggtt gaaaaaaatc
     1261 ttcgtcggga gggctgtcgc gtctccatag acttgcgaaa tgaaaagatt ggccttaaaa
     1321 tccgtgaggc agaaaaggca aagatccctt tcatgattgt cgtgggggat cgtgaagctg
     1381 aagcaaaaat gattgcggta cgacaaagga acgggaaaaa tttggggacg atgcctatta
     1441 ttgatatggt gacgcttatt cgggaagaca tgcccgagtc tgttaagaag ggttctttac
     1501 ttttcgaatg actcgtccgg tgacccccaa acaacgtatt aatcatttca tcaaaaatcc
     1561 tgaagtccgg gttattggtg ctgaaggtga acagcttgga atcctgaaga catcggaggc
     1621 gattcggcag gcgagggaaa ttggatatga cttggtggaa gtggctccta ccgcggaccc
     1681 tccggtctgc cgtattatgg attatgggaa atttaagtat gaacagagca aaaaagaaca
     1741 tcgcatgcga cttaaccaga aatccaccca ggttaaggaa gttaaatttc ggcctcggac
     1801 agacaagcac gacatggaga cgaaagtcag gcaaatacgg gacttcctgg aagaagggaa
     1861 taagaccaaa gttacggtta tgtttcgggg gcgagaaatg gccaatcaag agcttgggtt
     1921 caaagcgatt cagaaggtta ttgaggagtt aaaaggcgca ggaaatattg agagtcctcc
     1981 caggatggag ggacggaatc tttacatggt ggtcgccccg aagtaattgg ctaattgtgg
     2041 gagtccggca atttgtcggt cgttgaataa ggaaataaag tatggcaaaa atgaaattaa
     2101 aaaatcactc aggagccagt aaacgattta aacgaagcgg ctcgggtaag tggatgcggc
     2161 ggaaggcagg aatgcgtcat atcctcactt ccaagtcgcc tggagtaaaa tccagactaa
     2221 gcggtgcagc agaggtcaga aaagaagacc ggacttcgct gtctcggtta ctcccctata
     2281 agtaattttt caataataaa ggagaacgtg tcatgccaag ggtaaaaggt gggccacaaa
     2341 ctcggcagcg ccggaaaaag cggctcaagc tggcgagtgg ccaatatgga ggaaaaagta
     2401 agcttttccg gaccgctacc gaatcagtcg ataaggggca agcgtatgct tataccggac
     2461 gaaaacagag aaaacgtaat ttcagacagc tctgggtcac tcgcatcagt gcggcagttc
     2521 gagcgcaagg cgtcacctat agtcagttca tgaatgcgct gaagaaagcc aatgtgttgt
     2581 taaatagaaa aatgctttca gaaatggcga ttcacgatcc tcaaggcttt tctcaccttg
     2641 tgtcaagcac caagggagat ggacaacccg tccccgctta agcttcaggc tttcgtatct
     2701 cgaattcaaa cccggaagta ggcatatcga tttgccaggc ttcgagtact tccgggtgaa
     2761 actctccaat atatcccact gaatgcccat tacaacggat tcggccgact cgtccttcta
     2821 agaatgaggg atgagtgacc ggctccagtt cgtagggcca caccatgtaa tacatcagca
     2881 aatccagata gctatggatc tctgaaaaat ttgctccgga attggcgctg atggcagcta
     2941 tggataatgt cgtgcgagaa cccgcattgg cttccaaatc cagttgagcc gtttctccaa
     3001 cctcaaatag cagatgggga taaaagacgc gaggcgatag ggcttccacc cgaagcaggc
     3061 acggcaaaat ggaaggtctg agacaggcat aggtttggga catcaggtta tccacttcca
     3121 ccagttgcgc atactccgta tcagcaatac gcattctgtc taccaattcc tgatgagaag
     3181 ccatgatatt ggaaaaaatt tcctgaaagc caaatccgac cagaagatca cgaacacgat
     3241 cgctggtctg ttcttccacg gagagacttc caacggtgaa agactggggc ataatagggg
     3301 aaaaggaatc atagcctcgg gtaatggcca catcttccgc cacatccatc acatgcatga
     3361 aatcgtttcg aaaggcgggt aaggtgacgg atactgtttg cctggtggct ttcaccaggt
     3421 atccataaga ggctagggct tcctgaatag cctccgagcc aagcggcatt cctaaagcct
     3481 gttcgatcgc ctctagggaa atacgttgag attgattcat gctcaggggg gttttaacgg
     3541 ttgtgccgaa ctcggtttca tagggatacg taatattcac ggattcgatg gtcgccccac
     3601 gatcggctaa attacaggca aaaatattca aggccaacac caccatactc agatcggttc
     3661 ctgtgacttc cacaaataaa tcggtgtctc cctgctgcac ttcaccgagt tcccggctgt
     3721 tgataatggg aggaaaggac agcacctgtc catccgaatc ccataggagc ggcaacctgt
     3781 cgcattcagc gaggatcgat ccatattcaa ggcctttggg gtggacggtg agaatttcgt
     3841 gaggagtcat tttttcctca aatcccaatg gcgtaaatcg aatttcatcg ggtttcacga
     3901 gcccgtacgt gacaggaaac gaaatggaag agtaccgata aaggccaatg gaaaccgttt
     3961 cacgttttcg tccataggcg tcggccaatt tttcttgggt ttgaatgcat tggtcgaggc
     4021 cctcagctgt catggcatat cccaacgaga cgcaagccgc cacataaggt cgaacggttt
     4081 ccatgccttg ggccacttga atccgtcttt tcgccccctt cttatccgaa aagaaggagt
     4141 aagggggcat ccctttattt aaaacggatc gaatttgtcg ggcaatccct tccacgcacc
     4201 ataaatcagg ccgattggta tcttgaagct ccacgcgcac ttcacccgtg tcctgtgaga
     4261 cgtctttcac ctcccctttc acatagggca tccattgttc aatgtcggac atggaggcat
     4321 ttcgaccgac aagtcgacag aaatcttttt ggaaaatgga aatggttggc atcgtgggta
     4381 tttaaaattg acggcgcata ttgcggaccg cttctagatc ggaggtgaat aaatcgcgaa
     4441 tgtcctgtat gccaagggcg accatggcca tccgatccaa cccgagtccc caggcaataa
     4501 ccggaacggt cactcccaaa ggtatggtga cttcagagcg gaaaagcccc gcgcccccca
     4561 gttccatcca tccaagtttt ggatgacgga catgcaattc gacggagggt tcggtaaatg
     4621 gaaaatacgc tgggaaaaat cgaatctctt tggcttgtgc catttccgtg gcgaacaatt
     4681 gcaggagacc caagagtgtc cgaaagttga tatcggaacc gagcacgatc ccttccacct
     4741 gaaaaaaatc agtggcgtgc gtggcatcga cattgtcata acgaaaacac cgggcaatag
     4801 aaaaaaattt gccgggaatc ggcggatttt gtgccaacgt tcgagcggag actgcggtgc
     4861 cttggctgcg gagaactaag cgcttcgccc gctcgctgtc atattcgtat ttccatcctt
     4921 tcgatccact cgttccgcca tcccggtgga cttgggcgac ctggctgaga aatggctcag
     4981 gaatattctt agccatcttg ggatttttaa caaaatacac atcatggatg gcacgagcag
     5041 gatggaattg aggcataaag agtgcgtcca tatcccaaaa ttccgtttca atgagttcgc
     5101 cacgcatttc ctgaaatccc atgctcgtga gtttgtactt cacggtatcc agaaactccc
     5161 gataggaatg gcgtcggcca actgccaatc ggggaggtcg gagattgatg gagtatttgc
     5221 gaaaggacac gttccgccag gatccgtctt ttaagagttc tggggttaat tgtgaaattt
     5281 cctgggcggc gccttcccgt aaatgaggca gtagagtccg gccctccgct gtgaggacat
     5341 aagtccgttc ggtatgatca tcgatgcgaa agggttcttg cgtattgccg cgtttga
//
LOCUS       DAORAQ010000166         5364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2213_length_5364_cov_53.336410, whole genome shotgun sequence.
ACCESSION   DAORAQ010000166 DAORAQ010000000
VERSION     DAORAQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_5364_cov_53.336410"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKM72_11750"
     CDS             complement(<1..300)
                     /locus_tag="PKM72_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNP61507.1"
                     /translation="MGAADSSDNEMNDVGMQATRDSRPIHPVYVDGFWMDKTEVTNAQ
                     FTLFVDATGYVTVAERTPRAEDFPGAPTENLVAGSVVFSPPDHAVPLDTHFRWWSY"
     gene            329..502
                     /locus_tag="PKM72_11755"
     CDS             329..502
                     /locus_tag="PKM72_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61508.1"
                     /translation="MPSGAGSDGGDECAEPDEAFADGTDVFTDVSGGALGVAGTRPDF
                     AAVIPISQVMPTC"
     gene            879..1085
                     /locus_tag="PKM72_11760"
     CDS             879..1085
                     /locus_tag="PKM72_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61509.1"
                     /translation="MPLEARNPGIPMPRIFSGVWGVGVMDMLPGESRVGRNLGGRGGE
                     VVHLDVTRSRKHFAHGLWLGDELG"
     gene            complement(1430..2515)
                     /gene="ydiK"
                     /locus_tag="PKM72_11765"
     CDS             complement(1430..2515)
                     /gene="ydiK"
                     /locus_tag="PKM72_11765"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HNP61510.1"
                     /translation="MNSPFHTQDITRTVLAVLFIGGLIAGSLWILRPFLPAVIWATMI
                     VVASWPVLLRIQEWLWGRRWLAVTVMTCILLLIFVVPFSLAIVTIVTNSNIIAGWVEA
                     VGTLQLPPPPDWLQTIPLVGRQAAQTWGEWAASGQEDLANRLAPYAAGLVRWSVDQLG
                     SFGMIAMQFLLTVVMAAILFATGETVTESVRRFGRRLAGIHGENAVILAGQAIRGVAL
                     GVVGTAFVQATLGGIGLAVTGVPFATILTAMMFLLCIAQIGPLLVLLFGVGWLYWAGF
                     AGWATALLVWSIMVGTLDNVIRPILIKRGADLPLLLILTGVIGGLIAFGLVGIFIGPM
                     LLAVSYRLLQAWVCEELAEPAPNAVTP"
     assembly_gap    2723..2822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3298..4779)
                     /locus_tag="PKM72_11770"
     CDS             complement(3298..4779)
                     /locus_tag="PKM72_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNP61511.1"
                     /translation="MTTPISPSLYQINTRVWLTEISQSLGRRATLDDLPETELDRLAK
                     MGFDWIWFLSVWRTGPAGQQVSRSHHEWRREFEETLPDLHEKDIAGSGFAITGYTVHP
                     DLGGDTALARLRERLRKRGLKLMLDFVPNHTGLDHPWVETHPEYYIAGSELDLSRAPQ
                     NYTWAGRKNGDLLLAYGRDPYFSGWPDTLQLNYGNPATQEAMIGELIKIAGQCDGVRC
                     DMAMLVLPDVFERTWGIQAELFWPNATRRVREKSPGFCFMAEVYWDLEWTLQQQGFDY
                     TYDKRLYDRLREGQARPVRDHLRAEPGYQNKMARFLENHDESRAAATFSQDIHEAAAV
                     VTFLSTGLRFFHQGQFEGRRKRISPHLVRAPKESTDESLHRFYERLLTVLRQPAIREG
                     QWTLLECLPAWEGNGTSDNFLVFAWQGPKEERLLVMVNVAANPSQCYIRFPFPELMGR
                     TIRLKDQMGDASYDRNGNDLLSQGFYLDLPAWGYHVFEVNVGT"
BASE COUNT         1173 a         1447 c         1483 g         1161 t
ORIGIN      
        1 gtagctccac catcgaaaat gggtatcaag cggcacggca tggtccggtg gagaaaacac
       61 aacggaaccg gccaccaggt tctccgtcgg ggcacctgga aaatcctccg cgcgcggtgt
      121 gcgctccgcg actgtgacat agccggtggc gtcgacgaac agggtaaatt gggcattggt
      181 gacttcggtt ttatccatcc agaatccatc gacgtaaaca ggatggatcg gacgggaatc
      241 gcgcgtggcc tgcatgccca cgtcattcat ttcattatcc gaagaatcgg ctgcccccat
      301 ggagaattcg cccccaggaa tccagaccat gccatccggc gccggatcgg acggaggcga
      361 cgagtgcgcg gagcctgatg aagcatttgc ggatggcacc gatgtgttca cagacgtgag
      421 tggaggcgca ctcggggtgg ccgggactcg tccggacttc gcagcagtaa tccccatatc
      481 gcaggttatg ccgacttgtt gattcggcac ggatgacctg tctgattgcg ccgtgctgta
      541 ccaccagatt cccagtccca ggattatggc gaatgctgca gcgccaaggg gtatgaacgg
      601 gtgggatggc ttgggtccac cttctttttt caagcgacgg tcggatttca tgatgatctt
      661 acaaacattt ggagataacc tcggtagtcc gcttccatta cttttcgatt gtagggaata
      721 ctattgtaac atgcacgctc agggcatcgt cgacatacag gacggtggag gcgcggttcc
      781 cgttctgatg cgtgcatgat gttcagttcg accacgcttt catgcgccaa aatttcaaga
      841 gatagccccg gatcacgata tcgttggttt cgggttttgt gccacttgaa gcccgcaatc
      901 ccggcattcc catgccgcgg atcttttcgg gggtttgggg cgtgggggtg atggacatgt
      961 tgcccggcga atcccgagtc ggtcggaatc tcggcgggcg tggcggcgaa gtcgttcacc
     1021 tagatgtgac caggtcgagg aagcatttcg cccacggtct gtggctgggt gatgaacttg
     1081 gttgaggcgc attcggcagc gatgaacaat acgaagagac aggagtaaat atgaatacaa
     1141 gaattcatgg tgaatgtcct atggtgaagt ggtgggggtg gagttgcttc cgatagtcct
     1201 ggagttttta gaggacgaaa aataggacac cgattttcaa aacgatcaat ggttccatat
     1261 tacgcttctg ttctttctca actttatgcc aggccttttt cccagccatt tcggtatttg
     1321 aaataataga tgatacgttc cggcgcctgc ttgattaact ccttgaacac ggttcatgtg
     1381 ggctacatgt tgttggaaac gcattcttgg taaaacttgg cgagtagggt cacggcgtaa
     1441 ctgcattagg cgcgggctca gccagctctt cacagaccca ggcttgaagc aggcggtatg
     1501 agaccgccaa cagcatcgga ccgataaaga tccctaccag accgaatgcg atgagtcctc
     1561 caatcacccc tgtcaagatt aagaggagcg gcaggtcggc gcctcttttt atgaggatcg
     1621 gccggatcac attgtccaaa gtaccgacca taatcgacca gaccagcaga gccgtcgccc
     1681 agcccgcgaa tcccgcccaa taaagccatc caactccgaa cagcaacaca agtagcggtc
     1741 cgatctgtgc tatgcagagc aggaacatca tggctgtcag gatcgtcgcg aagggcacgc
     1801 cggtgacggc aaggcctatg cctcctagtg tggcctggac gaatgcggtc ccaaccactc
     1861 cgagcgcaac gcctcgaatc gcctgaccgg caaggattac ggcattctcc ccgtggattc
     1921 cggccagcct tcgtccaaag cgacgcacgc tttcggtcac cgtctctccg gtcgcgaaca
     1981 aaatagcggc catgactacc gtgagcagga actgcatggc gatcatgccg aaactaccga
     2041 gctggtccac ggaccaacga acaagccccg ctgcgtaagg cgcgagccta ttggcaaggt
     2101 cctcctgccc tgaggcagcc cattcgcccc acgtctgcgc cgcttgcctg ccgacgagtg
     2161 ggatggtctg aagccaatcc ggtggtggtg gcagttgaag cgtgccaacc gcctcgaccc
     2221 agccagcgat gatgttggag ttggttacga tggtgacgat tgccagcgag aacggcacga
     2281 caaagataag gaggagtatg cacgtcatca cggtgacggc gagccaccgc cgcccccata
     2341 accactcttg aattcgcaag aggaccggcc aggacgcgac gacgatcatc gtcgcccaga
     2401 tcaccgcagg aagaaacggc cgaagaatcc agagcgaacc ggcgatgagc ccgccaatga
     2461 acaacaccgc caataccgtg cgcgtgatgt cttgtgtgtg gaaaggacta ttcatgagcg
     2521 gaacccattc cctattccaa cgaatacgtc acaattcata ggaagcctct taacttgatt
     2581 tggtgtgtga tcacggctca attggcctgg atcgtgacga gcggggatgg gcctgctcgc
     2641 cggtatcgac catggtcata aagccgaaaa gccatgtgaa tgccatttcc cttttgcctc
     2701 cggaccatcc tgacgaagat acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnaaagccat gtgaatgcca tttccctttt gcctccggac catcctgacg aagatacccg
     2881 cgatgatatg ccaaaggatc acgggtctca aaacgattaa caacccatag taaacccaga
     2941 ggatttggag ggcgcccata atggcagcca tcctcggatt cattcccttg cttgacctcc
     3001 gcctgagcat tccgattaag tcgggaaggg cggcaaaggt ggttcctaag cccgccacaa
     3061 agccgatgat ttcagaccgt cgaaagttgt ggagtaacag gctttccgta tccggtggct
     3121 aactccccac agccagcaag aatgaacgcg ctgaatagaa cactaaggag ttgaagtctc
     3181 tttggaaata ccatggttat ctctattttc gtcaggcact ctctttgagt cctgactctt
     3241 tcgatcaatt cggcctccgt tagagccgcc actgtaccac tcctcttgcc tccacgatca
     3301 agtcccgacg ttcacctcga agacgtgata gccccaggcc ggcagatcga gataaaagcc
     3361 ttgtgagagg agatcgtttc cattgcgatc ataggaggcg tcgcccatct ggtccttgag
     3421 ccgtatggtg cgacccatca gttccggaaa cggaaagcgg atatagcatt ggctcgggtt
     3481 ggcggcaacg ttcaccatga ccagcagccg ctcctctttt gggccttgcc aggcaaagac
     3541 cagaaaatta tccgatgttc cgttaccttc ccacgcgggt aagcattcga gcagggtcca
     3601 ttgaccttca cgaatcgccg gttgccggag caccgtgaga aggcgttcgt agaatcgatg
     3661 aagggattcg tccgttgact ctttcggcgc gcggaccaga tgcggcgaga tgcgcttcct
     3721 tcggccttcg aactgcccct gatggaagaa tcgtagaccc gtcgaaagaa acgtcacgac
     3781 agctgcggcc tcgtgaatgt cctgagaaaa tgtggccgca gccctggatt cgtcgtggtt
     3841 ttccaggaac cgggccattt tattctggta gccgggttcg gcacgcaaat gatcgcgcac
     3901 cggtcgggct tggccttcac gcagacggtc gtagagtcgc ttgtcgtagg tataatcgaa
     3961 tccctgttgc tgcaaggtcc attcgagatc ccagtagacc tcggccatga aacagaagcc
     4021 cggagatttt tcccgcactc gccgggttgc gttcggccaa aatagctcgg cctggatgcc
     4081 ccaggtccgt tcgaagacat cgggaaggac gagcatggcc atgtcgcagc gcacaccgtc
     4141 gcattgtccg gcgattttga tcagttctcc aatcatcgcc tcctgggtgg cgggattgcc
     4201 ataattgagc tgcagcgtgt ccggccaccc ggagaagtag ggatcgcgtc cgtaggcgag
     4261 cagcagatcc ccgttcttgc gtccggccca tgtataattc tgcggcgcgc gggacagatc
     4321 tagttcgctt ccagcgatgt aatactcggg atgcgtttcc acccaaggat gatccagacc
     4381 ggtatgattg ggaacgaagt caagcatcag cttcaaaccc cgcttgcgga gtcgttcgcg
     4441 gagacgggcc agcgcggtat cgcctcccag atcgggatgc acagtataac cggtaatcgc
     4501 aaatccggaa ccggcaatat ccttttcatg taaatccggc agcgtctctt cgaactctct
     4561 gcgccattcg tgatggctgc gcgatacctg ttgtcccgcc gggccggttc gccagacgct
     4621 caagaaccag atccagtcga agcccatttt cgccagacgg tccagttcgg tctcaggaag
     4681 gtcgtccagc gtcgcccggc ggccaagaga ttgggatatc tccgtcaacc acacccgggt
     4741 attgatctga tagagtgaag gagagatcgg tgtggtcatg atggggaatc agcacgcgga
     4801 ccgcacggcg ctcaaccatc ccatcgaagt ctcccctaaa ttcccttgac gggatgggca
     4861 gcgcacgccg tggtggtgaa gagcagcaag acggcggcga gacaaagaga ctgcaaaggg
     4921 gggaactctt ttaggtgcgg gatgttgtcc tctgattatc tctggatatc ggagacgctg
     4981 ttcatttttg ctcaatctca ccttcgtcat gacggaatcc aggctcttcc agcgccgcct
     5041 gttaccgttc cggagcctcg gatgaggata aaggcaatga tatacatcat cttcggtatt
     5101 gtaaattgcc ggactgtgag cgatgcgcac tggtgacaac tagaccgatg atcaatgcgg
     5161 atatttatgg ctccccgcgt ggcggctttc atcaagctca actatgttga cggctgttgg
     5221 gatggtgcgg ctctttcggt tgaggggttt ggcttggcgg acccacattc ggtctgatcc
     5281 ggtttggtta cttgatccac ccctgttgct ggaatctggg cttgatctta tcggcgattt
     5341 ctttggcaat cgccttttcc cggc
//
LOCUS       DAORAQ010000167         5358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2220_length_5358_cov_58.912691, whole genome shotgun sequence.
ACCESSION   DAORAQ010000167 DAORAQ010000000
VERSION     DAORAQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_5358_cov_58.912691"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKM72_11775"
     CDS             <1..353
                     /locus_tag="PKM72_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNP61512.1"
                     /translation="SNHIRFRLGPDRVAIAMGARAKVPGERMTGEDIELYVCRQQGDE
                     QGAYERLIGDAMAGDAALFAREDGVEEAWRIVDPVLKMPTPVFEYAAGTWGPNEAQAV
                     VADIGGWHNPKEEE"
     gene            complement(653..1360)
                     /locus_tag="PKM72_11780"
     CDS             complement(653..1360)
                     /locus_tag="PKM72_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNP61513.1"
                     /translation="MTDNKLVEKNKWMIDPDDAVMLLIDHQSGLFQLVRDIDLPVLRS
                     NVTALTKVAHLAGIPTFTTASVPDGPNGPLIPEIHKYNPNAVYVPRTGQINAWDNPAW
                     VEAIKKTNRKTLLIAGTLTSVCMAFPTLSALVAGYKVFTIVDASGNWSKMATDITIAR
                     VVQAGAMPIDTYAVLAEIMSTWNRADAMEFAEIMTDHIVPPYRALIESYEKAQSVGKN
                     GRETKLEQLEAATTRKN"
     gene            complement(1409..2266)
                     /locus_tag="PKM72_11785"
     CDS             complement(1409..2266)
                     /locus_tag="PKM72_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNP61514.1"
                     /translation="MTTKNLTQIIPNTGQHWVGDGFPVTTMFSYDELGAEMSPFLLLD
                     YAGPAEFTPSDKPRGVEQHPHRGFETVTIVYQGEVEHRDNAGNSGKIGPGDVQWMTAA
                     RGILHEEKHGLNFTKNGGTLEMIQLWVNLPAKDKMTAPRYQEISNKDIPVVKLDEKGG
                     RLRVIAGDYTGTPGAAKTFTPINLWDIQLNKGQSLKLQLPDGFTTTLLVLKGTVLINE
                     TKNMNRKELALFERKGEQISLDATSDTLLLVFNGEPINEPIVGQGPFVMNTEEDIRQA
                     MTDYRGGRF"
     gene            2406..2738
                     /locus_tag="PKM72_11790"
     CDS             2406..2738
                     /locus_tag="PKM72_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNP61515.1"
                     /translation="MPTKKNVFCPVECTLDAIAGRWKVLIIHQLLEGTKRFNQLQREL
                     GGITHRTLTKQLREMEERGLVNRKDYKEIPPRVEYRLSPLGQSLESVLLAMHEWGESY
                     GRKVLQKK"
     gene            3403..4767
                     /gene="chrA"
                     /locus_tag="PKM72_11795"
     CDS             3403..4767
                     /gene="chrA"
                     /locus_tag="PKM72_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNP61516.1"
                     /translation="MNKPEAPPVAPPASPPTPVTFWQAFVFWLKLGFISFGGPAGQIA
                     IMHQELVERRRWISERRFLHALNYCMILPGPEAQQLATYIGWLMHRTWGGIVAGALFV
                     LPSLLILIGLSWIYIAFGDVPLVAGLFYGIKPAVTAIVVQAAQRIGSRALKNNVLWAI
                     AAASFVAILALNVPFPAIILLAGLIGHFGGGIAPDTFRAGGGHNQIDMSVGSTLIDDD
                     TPPPEHAHFRWTKLLKITVVGGVLWAAPMALLVANFGWQHTLTQMGWFFTKAALVTFG
                     GAYAVLPYVYQGAVGHYGWLTPTQMIDGLALGETTPGPLIMVVAFVGFVGGYVKVLFG
                     PEFVFLAGAVAAILVTWFTFLPSFLFILAGGPLVESTHGDITFTAPLTAITAAVVGVI
                     LNLALFFGYHVLWPHGFTGSFDWVSALIALAAAVALFRYRRNVIEVIAVCASIGLIIN
                     TLIL"
     gene            complement(4811..>5358)
                     /locus_tag="PKM72_11800"
     CDS             complement(4811..>5358)
                     /locus_tag="PKM72_11800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP61517.1"
                     /translation="HPTMSLHLIKSTGVQKTASLIDVGGGDSTLVDHLLDQGFEHLTV
                     LDISAAASDRAKDRLCDRANRVTWIEGDVTHFRSSKTYDVWHDRALFHFLTEAEDREK
                     YCETMHRAVSAQGHVIIAAFAYEAPPTCSGLPVVCYSPEFLTLAIGNNFELVESVEQI
                     HHTPGGNKQPFIYCRFMRRKP"
BASE COUNT         1167 a         1395 c         1396 g         1400 t
ORIGIN      
        1 catcgaacca tattcgtttc cggttgggtc cggatcgagt cgcgattgcg atgggtgccc
       61 gggccaaggt acctggcgaa cgaatgaccg gggaagacat tgagctctat gtctgtcgcc
      121 aacaagggga tgaacagggg gcctatgagc gcctcatcgg cgatgcgatg gcgggagatg
      181 ccgcgctgtt tgcccgggag gatggagtcg aagaggcctg gcgtattgtg gatccagttt
      241 tgaagatgcc cacccctgtc tttgaatatg cggcagggac gtggggtccc aatgaggcgc
      301 aggctgtcgt ggcggatatt ggtggatggc ataatccaaa ggaggaggaa tgaatcctta
      361 ccctcttttt cagagctcac acggttcgct ccacattcct ggaggtgcta attttattta
      421 gttgaccgaa tgaaatgtca tctttctatc aaccggatct tccggcggga taccgttgat
      481 gctatttggt tgcatgccgc cgattggtct gtgatctgcg ggagaagctg gtaagcttac
      541 caacttctca cgcacagcca gtgaaatgca ggtctagact tttgttattc gcaaattcga
      601 gtttcttctt tggtatagaa aaacccttgt tgaaaaattg ctcttaattt atttaatttt
      661 ttctggtcgt tgcagcctca agttgttcaa gttttgtttc tctgccattt ttcccaacgc
      721 tttgggcttt ttcgtagctt tcaatcagag cacggtaagg cggaacaata tggtctgtca
      781 taatttctgc aaattccatc gcgtccgcac gattccacgt gctcatgatc tcagccagca
      841 ccgcgtaggt atcaattggc atggctccgg cctgcaccac gcgagcaatg gtgatgtcgg
      901 tggccatctt gctccagttc ccggaagcat cgacaatggt aaaaacctta taacccgcga
      961 caagggcgct tagggttgga aacgccatgc aaacacttgt aagtgtgccc gcgattaaca
     1021 gggtctttct attggttttc ttaatggctt ccacccatgc aggattgtcc catgcattga
     1081 tctggccagt tcggggaaca tacaccgcat ttggattata cttatggatc tccgggatca
     1141 gcgggccatt cggtccatca gggacagacg ccgttgtaaa ggttgggatc cctgcaaggt
     1201 gtgccacttt tgtcagagcc gtcacgttgg atcgcaacac cggaagatcg atgtctctga
     1261 ctaactgaaa caggccgctc tgatggtcaa tcaaaagcat gaccgcatca tccggatcaa
     1321 tcatccattt gtttttctct actaacttgt tatctgtcat gcttgctcct tgttggtgct
     1381 ggtggttgat ctgcttccgc taaacatatc agaaacggcc tccacgataa tcggtcatgg
     1441 cctgccgaat atcttcctct gtattcatca cgaacggtcc ctgtccaaca atgggttcat
     1501 ttatcggctc cccattaaat accagcaata acgtatcgct tgttgcgtcg agggaaatct
     1561 gttcgccttt ccgttcgaag agagccaatt ctttcctgtt catgttcttc gtttcattga
     1621 tcagaaccgt ccctttcaga accaataacg tggtcgtgaa tccatccggc agttgcaatt
     1681 tcagggactg tcctttattg agctgtatgt cccatagatt aatgggagtg aaggtttttg
     1741 ccgcacctgg agtgccggtg taatcgcctg cgataaccct gagcctgcct cccttttcat
     1801 ccagcttcac aaccggaata tctttgtttg aaatttcctg ataacgcggc gcagtcattt
     1861 tgtctttagc cggcaagttc acccagagtt ggatcatctc cagggtgccg ccatttttcg
     1921 taaagttgag accatgtttt tcctcatgga gaatgccgcg tgccgcggtc atccattgca
     1981 catcgcccgg accgattttt cctgagttcc cggcgttgtc ccggtgttcg acttcgcctt
     2041 gatacacaat ggtgaccgtt tcaaagccac gatgcggatg ttgttcgact ccccgaggtt
     2101 tgtcggaagg cgtaaactct gcaggaccgg cataatcgag cagcagaaac ggactcatct
     2161 ccgctcccaa ttcgtcatac gaaaacatcg tcgtcaccgg aaacccatct ccgacccaat
     2221 gctgaccggt gtttggtata atttgcgtga ggttttttgt tgtcacgatg attccctctt
     2281 ttcatttggc actagcgtga ttgttcccca ttttttcttg tgggccacga tgatatataa
     2341 ttcttacagg atcattgtgc aagtacgcac ataaatgata ccgctcaaac agaaagagaa
     2401 tttcgatgcc caccaagaag aatgtgtttt gtcccgtaga atgcacactt gacgccatcg
     2461 ccgggcgatg gaaggtctta atcattcatc agttgcttga gggcaccaag cgtttcaatc
     2521 aattgcaacg ggagttgggc ggaatcaccc accggacgtt aacgaaacaa ttgcgtgaaa
     2581 tggaagaacg tgggttggtc aatcgaaaag actataagga aattcccccg cgggtcgaat
     2641 accggttgtc cccccttggc caatcgcttg aaagcgtgtt gctcgcgatg cacgaatggg
     2701 gagaatccta tgggcgaaaa gtattacaga aaaaataaat aattcatgaa cataaccggt
     2761 tttcaatgaa gcctaggaaa acatttgaat acgaaacacc tgcagggaaa tgtcatgtat
     2821 gtgttgcagc gaccaattga gctcacagcc agtagtggac acttgcgatt tcccaaaaaa
     2881 aggttttcga cacccaatca ttgagaaatt gtcaaaaatg atgttgttgc acaaaggatg
     2941 ggttctgcaa agcccggtgt taacccactt tcatgttagt acggcttcat ttgctgggct
     3001 caggtgcccg ttgtcacttg aatgggctta aatgaccata tcagtctcaa agtgctcagg
     3061 acttatctga ctggcttaca attgttttcc cccaatctat tggcaggaat acgcgaaaat
     3121 catccggctt tccttcctac tctattcact gataagtgtt tcggtcggat cggggtcagt
     3181 cgtctgaatg agaagacctc atggcgccca aagcaatctg gttcgcagag tcatgaaggt
     3241 tttgctagtg ttgagtgttc agatcatgcg aaccaatgaa tgggtctgac caatgcgagt
     3301 atggcgaact tctcccagca ccggcgccac tctcctggtg gcgacagcgt gcggggtctt
     3361 ggacggcttg cctgtcgccg ctgaacaaac cgtacccacc cgatgaacaa gccggaagcc
     3421 ccccctgtcg cacctcccgc atccccacca acgccggtga ccttctggca ggccttcgtc
     3481 ttctggctca aactcggatt catcagcttc ggcgggcctg ccggacagat tgccattatg
     3541 catcaggaac tggtcgaacg tcggcgctgg atttccgagc ggcgttttct gcatgcgctg
     3601 aactactgca tgatcctgcc cggcccggaa gcacagcaac tcgccaccta tatcggttgg
     3661 ctgatgcatc gaacatgggg cggaatcgtg gccggggcac tgttcgtcct gccgtcgctt
     3721 ttgatcctga tcggcttgtc gtggatctat atcgcgttcg gtgatgtgcc gctggtcgcg
     3781 ggtttgtttt acggcatcaa acccgccgtg acggccattg tggttcaagc cgctcagagg
     3841 atcggctcgc gggcgctcaa gaacaatgtg ctgtgggcaa ttgccgccgc atccttcgtg
     3901 gcgattcttg cactcaacgt gccgtttccc gccattatcc tcttagctgg actgatcggc
     3961 cacttcggcg gtgggatcgc tccagacacg tttagagccg gtggcggcca caaccaaatc
     4021 gacatgtcgg ttggctcaac gcttattgat gacgacactc cgccacctga gcatgcgcat
     4081 tttcgttgga cgaaattgct caaaatcact gtcgttggag gcgtgttgtg ggctgccccg
     4141 atggcgttgc tggtggccaa ctttggctgg cagcacacgc tgacgcaaat gggctggttc
     4201 ttcaccaaag cggccctggt aactttcgga ggggcctatg cggtgctgcc ctacgtgtac
     4261 cagggcgcgg ttgggcatta tggctggctc acccccacgc aaatgatcga cggtttggcg
     4321 cttggtgaaa ccactccggg gccgctcatc atggtcgtgg cttttgtcgg attcgtcggc
     4381 ggctacgtga aagtattatt cgggccggag tttgtatttc tcgccggggc ggttgccgca
     4441 atactggtga cgtggttcac ctttcttccc tcattcctgt tcattctggc gggaggcccc
     4501 ttggtcgaat cgacccacgg cgacatcaca ttcacagcac cccttaccgc catcacagcg
     4561 gccgttgtgg gagtcatcct caatctggcg ctgttcttcg gctaccacgt tctctggccg
     4621 cacggcttca ccggctcctt cgactgggta tcggcgttaa tcgcgctggc cgccgccgtg
     4681 gccctgttcc gctaccggcg gaatgtcatt gaggtcatcg ccgtctgcgc gtcgatcggg
     4741 ctgatcataa acacattgat tctgtgaggt gagtcatgaa gtggatttcc ggggacgatc
     4801 cccaaaaagt tcagggcttg cgtctcataa agcgacaata gatgaacggt tgcttgttgc
     4861 ccccaggggt gtggtggatc tgttcaactg actcaacaag ctcaaaatta ttcccaatgg
     4921 ctaaggtcaa aaattcaggg gaatagcaaa caacaggtaa gccgctgcat gttggtggcg
     4981 cctcataggc aaaggccgcg atgatcacgt gaccttgcgc actaacagcc ctgtgcatgg
     5041 tctcgcagta tttttctcga tcctccgctt ccgtcagaaa atgaaacagg gcccggtcgt
     5101 gccacacatc gtacgtcttg gacgaacgaa aatgggtgac atcaccttcg atccaggtca
     5161 cccggttcgc gcgatcgcac agtctgtcct tggcacgatc cgaagcggcg gcagaaatat
     5221 cgagaacggt aaggtgctcg aagccctggt cgagaagatg atcaaccagg gttgaatcgc
     5281 cccctcccac gtcgattagg cttgcggtct tttgaacgcc tgttgacttg atcaaatgca
     5341 atgacatcgt gggatggg
//
LOCUS       DAORAQ010000168         5303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2238_length_5303_cov_68.514101, whole genome shotgun sequence.
ACCESSION   DAORAQ010000168 DAORAQ010000000
VERSION     DAORAQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2238_length_5303_cov_68.514101"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKM72_11805"
     CDS             complement(<1..188)
                     /locus_tag="PKM72_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNP61518.1"
                     /translation="MDISFNDIKQKFQGCGLKTTPQRTAIYDALLRSTAHPTAEDLFA
                     QVAPQFPMMSLNTVYYTLG"
     gene            complement(331..1263)
                     /locus_tag="PKM72_11810"
     CDS             complement(331..1263)
                     /locus_tag="PKM72_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNP61519.1"
                     /translation="MSSRIVSLIPSGTEMVCALGCRAQLVGRSHECDFPSDITQLPIC
                     TQATVNSEESSQAIHAQVSERLQTALSLYEVLVDQMQNLRPDIIVTQTQCEVCAVSAN
                     EVQQALHNLVGSSPTLISLGAQDLSGVWDDLTRVAEGLGQQAAGQEFLKQAHQRMGDI
                     ANHSQQQLTPPTVACIEWMEPLMAAGNWVPELVHLAGGYSVFGQQGKHAPWITWEALA
                     ASDPDFLVLMPCGFSMARIQEEMPVMTSHPFWSQLRAVKNQKVYLTDGNQFFNRPGPR
                     LVESLEILAEILHPAHFQFGHEGQGWRQWVSKER"
     gene            1526..1762
                     /locus_tag="PKM72_11815"
     CDS             1526..1762
                     /locus_tag="PKM72_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNP61520.1"
                     /translation="MAAKAYILMKVKAGKVQDVLETLKALSGVEQAHACFGQPDIFGL
                     VNAPDDRALSNLIMAKIHTIPGVEETDTHIVVQD"
     gene            complement(2018..2497)
                     /locus_tag="PKM72_11820"
     CDS             complement(2018..2497)
                     /locus_tag="PKM72_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNP61521.1"
                     /translation="MESLLAWGHFLAGITWIGILYYFNFIQVPFLKAVTPETKAEAFK
                     HLVPNALWWFRWGALFTWIFGAALLMNQHRMGSAFMLQGQDAVIGMGAWLGTIMLLNV
                     WGIIWPNQKKVLGLKEATPEEKAKAGRMALLASRTNTLLSIPMLFFMANSGHASGLF"
     gene            complement(2791..3393)
                     /locus_tag="PKM72_11825"
     CDS             complement(2791..3393)
                     /locus_tag="PKM72_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNP61522.1"
                     /translation="MTPKPPIPLLRDFYDRPTLKVAKELLGKVLIKQTPTRMIKTKIV
                     DTEAYVGPKDKACHASKGRTKRTEIMFGPAGFTYVYLIYGMYHCLNIVTEQEEYPAAV
                     LIRGLEILEGDHSPDVPRRIDGPGRICRFLEIDRTQNGLDATIGTTLWIENHGLAVSP
                     KQIQTLPRIGVDYAGEWAKKLWRFCLPASTPPKTRRIIQK"
     gene            complement(3400..5124)
                     /locus_tag="PKM72_11830"
     CDS             complement(3400..5124)
                     /locus_tag="PKM72_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNP61523.1"
                     /translation="MKTDIAQQEARTALDRVKHYHEQTKHEFNRYARSLGHLDWANQP
                     NPFRRFEGASLIALPHLTLDEDPLSPAYESLFHPHSIPSQPITLNTLSRFFEYGLSLT
                     AWKAYNGTSWALRSNPSSGNLHPTEGYLLTGSLPQLALDPGLYHYAPKEHALEHRWAI
                     PSSLARSILQGLPSEGFLVGLTSIHWREAWKYGERAFRYCQHDIGHAIGTLRIAAATL
                     GWKLLVLSGTTDEAIARLLGLTRKQDFHQAEPEYPELLAAVWPRNLQPLPTLNLSFHE
                     INDAMLSSGEWKGTANRLSKDEPVLWEIIDLVTDASWKSTQEPEFITFHSPTAPTSEP
                     ENGKTEPLAGHIIHQRRSAVAFDGRTGLSADRFFTMLQRIMPNGELPLPDRPMPWDAI
                     PWNPTIHLFLFVHRIDGLPPGLYWLNRSPNHRHISSFKSAMHEQFVWSTPEACPRDLP
                     LYLLEEGNAQNLAKQLSCGQNIAGDSAFALGMVAEFDDSLDTFGPWFYKRLFWETGLI
                     GQVLYLEAEAAGIRSTGIGCFFDDPVHRVLGVHPQTKWQSLYHFTVGGPLDDGRLTTL
                     PPYGPHVT"
BASE COUNT         1303 a         1272 c         1444 g         1284 t
ORIGIN      
        1 ccaagtgtgt agtagacggt gttcagagac atcatgggaa attgcggagc gacttgggca
       61 aagagatctt ccgctgtcgg atgcgcagta ctgcggagga gcgcgtcata aatggccgtt
      121 cgctgaggag tcgtttttaa tccgcatccc tggaacttct gcttgatgtc attaaaagaa
      181 atatccattt tattcctatt taagaataat tactatttaa caaagatctc acgaatgggt
      241 caaccgaagt ccgccgattg cattggcata agcaaagcca agacgaaatt gggagggtcc
      301 gtacttcggt gaatcgtgcc ttagagatag ttaacgttct ttagaaaccc actgccgcca
      361 tccttggcct tcgtgtccaa attggaaatg ggcgggatga aggatctcag ctaaaatttc
      421 gagggattca actaggcgcg gtcccggacg gttgaaaaac tggttcccat ctgtcaggta
      481 cactttctga ttttttaccg ctcgcaactg tgaccaaaaa ggatgggatg tcatgaccgg
      541 catttcttct tgaatgcgag ccattgaaaa cccacacggc atcaaaacca ggaaatccgg
      601 atccgaggcc gccaaggctt cccaggttat ccagggggca tgtttcccct gctgtccaaa
      661 gaccgaataa cctccagcca gatgaaccag ttcgggtacc cagttgccgg cagccatcag
      721 cggttccatc cattcgatgc aggcgacagt ggggggagtc agttgttgtt gggaatggtt
      781 ggcaatgtcg cccatgcgtt ggtgggcctg tttcagaaat tcctgcccgg cagcttgctg
      841 tcctaatcct tccgccactc tggtcagatc gtcccaaacc cctgataagt cctgtgcgcc
      901 caaggaaatc aatgtgggtg acgacccaac taagttgtga agggcctgct gcacctcatt
      961 cgcactcact gcgcacacct cacactgggt ttgggtgacg atgatatccg gtcgtaagtt
     1021 ctgcatctgg tcgaccagga cctcatacaa ggagagggct gtctgaagac gttccgaaac
     1081 ctgggcatgg atagcttggc tgctttcctc agaattcacc gtggcttgag tacaaatagg
     1141 cagctgggta atgtcagaag gaaaatcgca ttcgtgtgac cggccgacaa gctgagcgcg
     1201 gcagcccaat gcgcagacca tttccgttcc gctaggaatt aacgacacaa tgcgagacga
     1261 cataatattt ccttgaaggt gaacaggaat agggagaaag aattgctcat cctacctgag
     1321 gaaggcaggg tgtccgcaac ttcttttcgg ttgcgtaagc tgggcgagag ggagccggaa
     1381 gacaatggaa gctgccttcc atctatggta caaaggtacc ttcaccatga tttggcaaaa
     1441 caatatcgat catgtctggt gtataccaaa atcaacgttg cctttgatca cagaaacctg
     1501 atccgcattt atcaaggagg tatgtatggc agccaaagcg tatattctca tgaaagtcaa
     1561 agcaggaaaa gtccaggatg tgttagagac cctcaaggcc ctttctggcg tggaacaggc
     1621 tcatgcctgt ttcggtcagc cggacatctt tggtctggtc aatgcgcctg atgaccgcgc
     1681 attgtccaac ctcatcatgg ccaaaatcca cacaatcccc ggagtggaag aaaccgacac
     1741 ccatatcgtc gtccaggatt agtttttcct tatccttgcg gtagaagcgg aggccggctc
     1801 aaggggtggg agcaggagca ggggacgaac tgaggattct ggatgagatg aaaaaccccc
     1861 tccagatcct attcagttgc tccctcgtac ttaccatcct tccaggcgct tcttaagccg
     1921 ggaaagaacg agagagaaga ggatgctata aagaaaaagc cggggtcttt agaccccggc
     1981 ttttgttgtg attccgattc gatatgaaat attgagatca gaataatccg gatgcatggc
     2041 cggaattggc catgaaaaat agcataggta tggacagtag ggtattggtg cgtgacgcca
     2101 atagggccat ccgcccggcc ttggcttttt cttcaggtgt agcttccttg agcccaagga
     2161 cttttttctg attgggccaa atgattcccc acacattcag caacatgatg gtacccagcc
     2221 aggcgcccat gccgatcacg gcatcctgtc cctgcagcat aaaggcactt cccatccgat
     2281 gctgattcat cagtaaggcg gcgccaaata tccaggtaaa cagggctccc cagcgaaacc
     2341 accagagcgc attgggcacc aggtgtttaa aggcttcagc tttggtttcg ggtgtgacgg
     2401 ctttgagaaa tggcacttgg atgaaattaa agtaatagag aattccaatc caggtaatgc
     2461 ccgcgaggaa atgtccccac gctaataaac tttccataaa atgtcctcgt attcgttagg
     2521 ttgattaaat taattgttgg aggtgagagg tgtcacacca gcattttgac caggccgtac
     2581 aggaccacgg tcaatacaaa gccagcaatg atggtgccag agatcgtatc gagcggattg
     2641 cccattgtct tcctccttta ttgttcgtag aaatgagaag cgggttgtgt catgtggctt
     2701 acggaatgta gccaacaact atctggtgcc ttagcgtaac gtagaccggc tatcttgttc
     2761 aagatatctg acaaaaggat tcgcaatcat tcacttttgg atgatccgac gcgtctttgg
     2821 tggagtagaa gccggcagac agaaccgcca cagtttcttt gcccattctc cagcatagtc
     2881 cacgccaatt cgaggcagtg tctgaatctg ttttggcgag acagccagac catggttttc
     2941 gatccaaagt gtggtgccga tggtggcatc caatccgttt tgggtgcgat caatttctag
     3001 aaatcgacag atccggccgg gaccgtcgat gcgtcgtggc acatccggcg aatgatctcc
     3061 ttccaggatt tccagtcctc gaattaagac tgcggcagga tattcttcct gttcggtcac
     3121 gatattaaga cagtggtaca ttccataaat cagatacaca taggtgaagc cagccgggcc
     3181 aaacatgatt tctgtccttt ttgttcggcc ttttgaggcg tgacaggcct tatctttggg
     3241 tccgacatag gcttccgtat ccacaatttt agtcttaatc atacgggttg gagtctgctt
     3301 aatcagtact ttccccagca attcttttgc gaccttcaac gtcggtcgat cataaaaatc
     3361 tcgtaataag gggattggag gctttggggt cataaggaat taggtgacat gagggccgta
     3421 gggcggcaga gtggtcaagc gtccatcgtc aagcgggccg ccaactgtga aatgatagag
     3481 cgattgccac ttggtttgag gatgtacgcc gagcacccga tggaccggat catcaaaaaa
     3541 acagccgatc cccgttgacc ggattccagc cgcttcggcc tccaaataga gcacctgacc
     3601 aatcagcccg gtttcccaaa acaaccgttt gtaaaaccac gggccgaacg tgtctaagct
     3661 gtcatcgaat tctgccacca tcccgagggc gaacgcactg tcacctgcaa tgttctgtcc
     3721 gcaactgagt tgtttggcca ggttttgggc gttgccttct tctagcagat agagcggaag
     3781 atcgcgtggg catgcttcag gagtcgacca gacaaactgc tcatgcatcg cgcttttaaa
     3841 agacgagatg tgccggtggt tcggacttcg gttgagccaa tacagacccg gtgggagccc
     3901 gtctatgcgg tgaacgaaca aaaagaggtg aatcgtggga ttccagggaa tggcatccca
     3961 gggcatgggg cgatcaggta agggcaattc tccatttggc ataattcgtt gtagcatagt
     4021 gaaaaaacga tcggcggaga ggccggtccg tccatcgaat gcgactgcgc tccgtcgttg
     4081 atggatgata tgaccagcca ggggttccgt tttcccattt tcgggttcgc tcgtgggagc
     4141 agttggggaa tgaaaggtta tgaattcggg ttcttgagtt gatttccatg acgcgtcggt
     4201 gactaaatcg ataatttccc acaaaaccgg ttcatctttg ctcaaacggt ttgccgtacc
     4261 tttccactct cctgaggaca gcattgcatc attgatctcg tgaaatgaca gattcagcgt
     4321 gggaaggggt tgaaggtttc ttggccagac ggccgccaat agttccgggt attctggttc
     4381 ggcttggtgg aagtcctgtt ttcgagtcaa acccaagagc cgtgcaatag cctcgtccgt
     4441 cgtgccactc agtaccaaca gtttccatcc taaggtggcc gcggcaattc gcaacgtgcc
     4501 gatagcatgg ccaatatcat gttggcagta tcgaaaggcc cgctctccat atttccaggc
     4561 ttcccgccaa tgaatagatg tgagacctac cagaaatccc tcagagggca ggccttgtag
     4621 aattgatcgg gcgagtgagg atggaattgc ccagcggtgt tcgagggcat gttccttcgg
     4681 ggcatagtgg tagagccctg ggtcaagggc aagttgaggc agagaacccg tcaagagata
     4741 accctcggtg gggtggaggt taccgcttga gggattactc cgcagtgccc agctggtccc
     4801 gttgtaggct ttccacgccg tgagtgataa cccatattcg aagaagcgag agagggtgtt
     4861 gagggtaatg ggttgtgagg gtatcgaatg tggatgaaag agagactcat aggctggcga
     4921 aagcggatct tcgtcgagtg tgagatgagg aagcgcgatg agcgaagcgc cttcgaatcg
     4981 tcgaaagggg ttgggttgat tcgcccaatc caaatgtcct aacgagcggg cgtaccggtt
     5041 gaactcatgt ttggtctgct catggtagtg cttaacgcga tcaagagcag ttctggcttc
     5101 ctgttgggcg atgtcggtct tcataaaggc aagtgtagca ccgtgagagg ggagtggaac
     5161 aagaaggcct gtcccgcctc gacgggtcgg ctttagaacc aggtcgattt aggcgggagg
     5221 ggtctacacg tccagattgt cagccagtcc ggcattggcc atcaaaaatt cataccgggc
     5281 ctctgtgcgt ttgcccatca aat
//
LOCUS       DAORAQ010000169         5303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2239_length_5303_cov_67.301639, whole genome shotgun sequence.
ACCESSION   DAORAQ010000169 DAORAQ010000000
VERSION     DAORAQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_5303_cov_67.301639"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1459)
                     /locus_tag="PKM72_11835"
     CDS             complement(317..1459)
                     /locus_tag="PKM72_11835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP61524.1"
                     /translation="MKKLTVLYVYLGFYRDAGGEHSPLGLAEELDRERFSFEIVTLMP
                     TTSVIGAAVQATGCKIHELGLGKPHLTDPVGMIKVIHAYYKTFRRLKPDIVQTQSGFC
                     NIWGRLAAKLAGIPVVIATVNFDEPPPRWFLRPFMHAMHRWLSTSTAAYVCIAKHLMK
                     EQLRPYEWSRAEIIYQFFDLNRFLGGRPHLPEVRAFTDPARPVLGIVGRLEHEKGHQV
                     AITAMRKIVDAVPGARLQIIGAGSLEKELRNQVQALRLSDHVEFMGHRTQICEMMSHL
                     DLLLIPSYNEAFGLVVLESIAAGVPLLGSRSGAFPEILENGKYGMLVDPANPGALADA
                     ALAIIANPAPALVKLKEARAQVLKRYTKEESARRHAGLYARLAEKL"
     gene            complement(1853..2479)
                     /locus_tag="PKM72_11840"
     CDS             complement(1853..2479)
                     /locus_tag="PKM72_11840"
                     /inference="COORDINATES: protein motif:HMM:NF039960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61525.1"
                     /translation="MMKAKGIPGLLAMAIALLAGCAAMPPNHQENLCKIFDQYPEWYD
                     AAKESQDRWGTPPHILMAFVKQESSFRHDAMPPWDWFLFIPLGRQSSAEGYAQAKDET
                     WEEYEDETDGFFQSRSTMEDALDFIGWYNHKSSRELGISKWDPKHLYLAYHEGRGGYA
                     RGSYKRKPKVVRIANRVDWQAKEYGAQLRQCEHRFRCRHWYQFWPFCS"
     assembly_gap    3037..3136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3155..4054)
                     /locus_tag="PKM72_11845"
     CDS             complement(3155..4054)
                     /locus_tag="PKM72_11845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP61526.1"
                     /translation="MSLAKTVAHGPIMIQVSVIVPFYNSERYIQRCVEGLLQQSFPRE
                     AYELILIDNNSTDSSAEKARQYPGIRLLTEPKQGAYAARNRGLQEAKGEILAFIDADC
                     VPAHDWLQNIMMAMEQPNLSIVLGQREYGGDSLVLSMFAAYEDEKHHYVFHSRKKELY
                     YGHTNNMAVKKHLFDELGPFVERARGSDTVFVRQSVNKYSCEVVRYCPQIRVRHLEIE
                     TPKNLYQKFFIYGRSRRSHRLIVEQEPLSNKERLQVFRNVVRTRQYSWMESMMFILYL
                     GIGLAYWNLGAISLAREEKDPVE"
     gene            complement(4065..>5303)
                     /locus_tag="PKM72_11850"
     CDS             complement(4065..>5303)
                     /locus_tag="PKM72_11850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP61527.1"
                     /translation="VIDSCSWVTTFTINEMVEIQRAGHQIILAPLYSGGPSPTHYGKA
                     EHVKPEAVLPDSPFDLHVVGLALLMLLKRPWRVLKTLAALHWAAGLNPYAHISNAIWT
                     PKALATAWRLEKMNVDRIHAPFATRSATCAGIAGRVSEIPFSFTAHAYDIYCTSLQLR
                     NDTLRWKIRHATQVFGISEDGIRLLRRMAPDCPHIHLVHVGIPLTLFTQRPAPPHHGV
                     LQLLCVASFVEKKGLDTLLDACKVLCEKNLKFYLRLFGNGPLKPMLEQQISDLGLLDK
                     VSLGGPITQNEVLEQMTKSHLIVMPCRKDGTGNMDGIPTVFMEAMAVGRPVISCPIGG
                     ISELVRDGETGKLVPSNDPHALAAVIMQLGEDQALRNRLVQQARALVEKHHDIRTTVT
                     LMLRHMNQSQKCGQQKGQCE"
BASE COUNT         1269 a         1270 c         1331 g         1333 t
ORIGIN      
        1 gctatgccaa gagtgcctcc tcgaccggcg acccggcttc ggcgggatac acggagcggt
       61 gtcatatcac acgggaattg ctcaaagata atctcctcct tgcgctcatt tttacaatgg
      121 ctggtgttaa cgcgggtggg tcagtccaac atgcttgtcc catgtaatcc tgtaaaccag
      181 gataacgcta tcgacctgga gtggaaggca accatcctcc atggtgtgga agggcaatca
      241 atccatcgga gcaaactctg aatggaaagc agtgaagcaa attattccat gttggaagat
      301 ttttagagaa tttctcttaa agtttttcgg ctaaacgcgc ataaagaccg gcatgtcgcc
      361 tggcgctttc ctctttcgtg tatcgcttga gcacctgtgc ccgagcctct ttcaacttga
      421 ccaatgctgg ggcagggtta gcgatgatcg caagtgcagc gtctgccaac gcgccaggat
      481 tggctggatc gaccaacata ccgtatttcc cattttcaag aatttctgga aacgccccgc
      541 tacgactacc caataatggt accccggcgg caatcgactc taacacgact aagccaaacg
      601 cttcgttgta agaggggatc aacaagaggt ccaggtggga catcatctcg caaatttgag
      661 tacggtgccc catgaactcg acgtggtccg agagtcggag ggcttgaact tgattcctga
      721 gttctttttc caagctgccg gctccgatga tttggagtcg agcgccgggt acggcatcga
      781 caattttccg catggccgtg atggccacct gatggccttt ttcatgttcg agccttccga
      841 caatgccgag gacgggacgg gcgggatccg tgaaagcccg aacttcgggt aaatgagggc
      901 ggcccccaag aaagcggttt agatcgaaaa attggtagat gatttcggca cggctccatt
      961 cgtagggtcg taactgttcc ttcatcaaat gtttggcaat gcagacgtag gctgctgtgc
     1021 tcgtcgacag ccagcgatgc atggcatgca taaacggtcg caggaaccag cggggtgggg
     1081 gttcatcaaa attgaccgtc gcgatgacaa caggaatgcc ggccagtttg gcagccaggc
     1141 ggccccatat attgcaaaac cctgattgtg tctgaacgat atccggtttg agccgtcgga
     1201 acgtcttata ataggcatgg ataactttga tcatgccgac agggtctgtg agatggggtt
     1261 tgcccagccc gagttcatgg attttgcaac cggtcgcctg tactgcagca ccaatgacag
     1321 aggtggtcgg catcaaggtg acgatctcaa agctgaagcg ctcccgatcg agttcttcgg
     1381 ccagccccag cggagaatgt tctcctccgg catcacggta aaaaccgaga tagacataca
     1441 aaaccgttaa ttttttcatg ttagtcgaaa agtctggatg gttcgtttac attgcccaag
     1501 taggataaat taaatgaagg gttgtagtca cactgtcgcc tggtgttttc atacgaaggg
     1561 ggattggaat ggatttgtag gaatggaaat gagggtgttt ggtatttgta ttgaactcgg
     1621 taatatttat accggaattg actgtgaggt catatgtgca aaaaagtaca cgttccatgc
     1681 gaggcctctt caagtcgcct ttgtccgagg tgaaggactg ctatctgacg ctctatctac
     1741 aagtttgaaa agtgtggatc agcaataaga aacctcagta actggatttt gatcaattcg
     1801 ttggcgaaac caggattatg gaaacttatt actctgatcg gaagcttcag catcaactgc
     1861 agaatggcca gaactgatac cagtgtcgac acctgaaccg atgttcgcat tggcgtaact
     1921 gagcgccata ctccttggct tgccaatcca cacgattagc aatgcgcacg accttcggtt
     1981 ttctcttgta gcttccccgc gcatagcctc cacgaccttc atggtaagcc aggtacaggt
     2041 gcttgggatc ccatttggaa atgccaagct ctctgctgct tttgtggttg taccagccga
     2101 tgaagtccag ggcgtcctcc atggtcgacc ggctttggaa gaacccgtcg gtctcatcct
     2161 catattcttc ccatgtctca tctttagcct gggcatagcc ctccgctgac gattggcgtc
     2221 cgagaggaat gaaaagaaac caatcccatg ggggcatcgc gtcgtggcga aacgacgact
     2281 cctgtttcac gaaggccatg agaatgtggg gcggcgtgcc ccacctgtcc tgagattctt
     2341 tggccgcatc gtaccattcg gggtattggt cgaaaatttt gcacaggttc tcctgatggt
     2401 ttgggggcat tgccgcgcat cccgccaaca gtgcgatggc catcgccagc agaccaggga
     2461 ttcccttcgc cttcatcatg tggttgcccg gaattccaat ggatattgtt accttagaag
     2521 gtgcatgtcg aatgccacag ggcttttcga cgagtcagcg ggtgattctt cggtgttgtt
     2581 gcaacattca caaacgctga tatctgtgag agatcgtttg caagtggcat gaaaactttc
     2641 cagaaattac actgcccgcc taaatcatcg gttcaacgcc cttcatctga ttcttgcatg
     2701 agaaataaac gcatggggct cccggccccg agtatatcaa tggtaacaac atttttccgt
     2761 agcgggattg agtgtttttt ataccttctt tccgcgcatg tacccttatc aattaatttt
     2821 accctggtga tgctatttct tttggtgcga agttttagaa atcgaggagg gaaggggaga
     2881 caattgaaaa ggataaggag gattggtgga ccggttggcc gaaccagacg aattgacagg
     2941 aggggcttcg tgtggcgtta ggattaattt atggttgaaa gccttggttg tgaaccacat
     3001 tccccaaaaa tttaatggaa tgggtagaaa atcttcnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnccat ccatccatgt ttctttattc aactggatct ttctcctccc
     3181 tggcaaggct gattgcaccc aggttccagt aagcaaggcc gattccgaga tacagaatga
     3241 acatcatgga ttccatccaa gagtactgcc gggtacggac gacattgcga aacacctgca
     3301 gccgttcctt attacttaac ggttcttgct ccacaataag tcgatggctt cttctgctac
     3361 gtccataaat gaaaaacttt tggtacagat tctttggggt ttcaatttcc aaatgccgga
     3421 ctcgaatctg tgggcaatac cgaacgacct cacaagaata cttattgaca gattgtcgaa
     3481 caaagaccgt gtcgcttccc ctggctcgct ctacgaaggg acccagctcg tcaaacaggt
     3541 gtttttttac ggccatattg ttcgtatgcc cgtaatagag ttcttttttc ctgctatgga
     3601 acacgtaatg atgtttttca tcctcgtacg ctgcaaacat cgacagcacc agagagtccc
     3661 cgccatattc tctctgaccg agaacaatgc tgaggtttgg ttgttccatt gccatcatga
     3721 tgttttgcaa ccaatcgtga gcggggacac aatccgcatc gataaaggcc aggatttcgc
     3781 ccttcgcttc ctgtagccct cgattcctcg ccgcatacgc gccttgtttc ggttctgtca
     3841 gcaatcgaat gccgggatat tgtctcgcct tttcagcaga ggaatccgta gagttattat
     3901 cgatcaggat cagttcgtag gcctcccgtg ggaagctttg ctgaagtaat ccttcgacac
     3961 atctctggat ataccgttcg gaattataaa agggtacgat gacggagacc tggatcatga
     4021 ttggaccatg cgcgacagtc tttgcaaggg acatgccctt gtcgttactc gcattgtccc
     4081 ttctgttgac cgcatttttg agactggttc atatgacgaa gcatcaaggt taccgtcgtc
     4141 cgtatgtcat ggtgtttttc gaccaaggcg cgagcctgtt ggaccaggcg atttcgaaga
     4201 gcctggtctt ccccaagctg cataattact gcagccaagg catgtggatc attggaaggc
     4261 accaatttac ctgtttctcc atcccgcacg agctctgata tgcctccaat ggggcaactg
     4321 atcacggggc gtccgacggc catggcctcc ataaaaacag tcgggatccc atccatattc
     4381 cctgtgccat ctttacggca tggcatgaca ataagatgac tctttgtcat ttgctccaac
     4441 acttcgttct gtgtgatggg accgccaagt gaaaccttgt caagtaagcc caaatcggaa
     4501 atttgctgct ccaacattgg ttttaacggg ccattaccaa ataagcgcaa ataaaatttg
     4561 aggtttttct cacacagcac cttgcaggca tcaaggagcg tatccaagcc tttcttttca
     4621 acaaagctgg ccacgcataa aagctgaaga actccatggt gaggaggagc aggtctctga
     4681 gtaaataatg tcaatgggat tcccacatga acaaggtgta tgtggggaca atcaggtgcc
     4741 atccggcgga ggaggcgaat cccatcttcg cttatgccaa acacctgggt tgcatggcga
     4801 atcttccatc tgagcgtgtc gttacgcagt tgaagcgagg tgcagtaaat atcgtaagca
     4861 tgggcggtga atgagaaagg aatctcactg acacgtcccg ctataccggc gcaggtggct
     4921 gaccgggtgg caaacggtgc atggatccgg tctacgttca tcttttccag gcgccatgcc
     4981 gtggccagag ctttgggggt ccatatggca ttactgatat gcgcataagg gtttagccct
     5041 gcagcccaat gaagggcagc aagagttttc aagacgcgcc agggacgctt caggagcatc
     5101 aaaagggcta agcctacgac atggaggtca aacggagagt caggcaggac ggcttcaggt
     5161 ttcacatgct cagcttttcc ataatgagta ggagagggcc cgcctgaata aagaggcgcc
     5221 agaattattt gatggcctgc acgctgaatc tctaccattt cattgatcgt aaaggtcgtg
     5281 acccatgagc aggaatcaat aac
//
LOCUS       DAORAQ010000170         5300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2243_length_5300_cov_56.487893, whole genome shotgun sequence.
ACCESSION   DAORAQ010000170 DAORAQ010000000
VERSION     DAORAQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2243_length_5300_cov_56.487893"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKM72_11855"
     CDS             <1..887
                     /locus_tag="PKM72_11855"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNP61528.1"
                     /translation="RKYLRGLYRSREDEQDYRVPLFVGGWTNRFAGTHLHISVANRKL
                     TKQEATALSWHLHDQLPLLIATGANSPIWDKKVTGKASNRFLRGNATYFTPTKRGDLT
                     SVDTRELVYSKGRKTKPPTLEIRVFDSNIPEFVVANLCLVKAICLRWLRGKAAANRMS
                     HADYLLARTEAATKGMKANIPWKREWVPAPEYLDRFLWEHREEIDDMDIPEEIYEVLR
                     LFKRRYNGTRLIHDAVALAIREHPQTWQRRFAKRYRSGLGHLLSGNTLQDFATELGVP
                     FPSTERVWLGRKRASIDE"
     gene            880..1626
                     /locus_tag="PKM72_11860"
     CDS             880..1626
                     /locus_tag="PKM72_11860"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNP61529.1"
                     /translation="MSKRFPAPLIGITCEAVSNRKDFANYDLLCDHRYAAAVTEAGGH
                     PVLLPINHRETLRNRYLEGIDGLVIVGGDDLDPSWYGERPKKRTKVAFKNRSKFEAWL
                     YKAGKTRRLPILGICYGMQLINVLEGGTLHQHIHPPKQGYHVDHEGKKNGLHPIKVIQ
                     GTRLATILGKGRKVVATEHHQGVRTLAPDFIPAAIASDGLIEAMEHPRYPHIFTVQWH
                     PERQLRSQTTQKLLRAFVRSCQHYQRTRNP"
     gene            1993..3603
                     /locus_tag="PKM72_11865"
     CDS             1993..3603
                     /locus_tag="PKM72_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP61530.1"
                     /translation="MISWHHPWKLQAKASLIIILIVGGAILVTELLHQYYVAELAKDV
                     IHSKSLVLLRQIGARFQSEKHFHNATLREKYLNDLMSRNPDLIFLRVYGKGTKADDPP
                     ILLTQVGNTEDTSSEIPLMVTHTFQFEEPQGRFHNQPGDDRLKLSTPLAIQGKTTGVI
                     YAEYWTGQLTTITRFFLNWSLIISLIMGFGIVVALNFFLYRHVIRPLGRLKTGLAEVE
                     KGQWATLVSIEKTDEIGDLARQFNSMAQQIQEVMRENASLNQALAQARDVLQTRVEDA
                     TAELRQRNEELAAVNERLSIAQRDILRQQRLAVLGQLVATIAHKIGTPLTAIFGHLQL
                     LQEDPHLPQEVLERVRTMLKQTDRLTHSIQDLLTFTRTPTISVEQVSLPTLIDQSLLL
                     FRPLLQEHGIQGLTHFSPDLWPIQGDAFHLQEAINNLIDNAIDAMPDGGHLTIQALNA
                     EDTPHAGPEVTVDIHDSGPGIPREHIEKIFDPFFTTKSVGSGTGLGLAITTEIIQLHK
                     GHITVQSEEGQGTRFLIRLPAWRIPDHA"
     gene            3596..4954
                     /locus_tag="PKM72_11870"
     CDS             3596..4954
                     /locus_tag="PKM72_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP61531.1"
                     /translation="MHKATILIVDDEPDTLTLLREIMEKEGYQVHTATSGQAALHTFV
                     DQTPDVVLSDIQMPHMDGLALLAETRKRSPLTQVILLTAYGSLSTAVEGIKAGAFDYL
                     SKPFALEEIRSVVRRACDHKHTLEEARVSIQPLLPKGNGINQIQGNSPPMVAVYKLIA
                     RVAPTESTVLIHGETGTGKELIARAMHANSLRNDGPFIAVDCGTLTENLLESELFGHE
                     RGAFTGAITLKKGLLESANRGTCFLDEIGDISPNLQSKLLRVLQEHEIRRVGGMESIK
                     VDVRIIAATNKHLKKLVESGKFREDLYYRLNVVTLHLPSLRERVEDIPTLIDYFLTKY
                     TQLNNKVITGVHPQALTLLTNYSWPGNVRELEHTIERAVVMTPHSLIMSHDLPLALVA
                     PPALPDQSASHPDWKTLGQLEREHILKVLDAQQGDENRTSELLGIHRKTLQRKLKEYG
                     LR"
BASE COUNT         1400 a         1591 c         1221 g         1088 t
ORIGIN      
        1 tgcgaaaata cctgcgcgga ctgtatcgca gtcgggaaga cgaacaggac tatcgcgtgc
       61 cgttatttgt gggaggctgg acgaaccggt ttgcgggcac ccacttgcac atctctgtcg
      121 ccaatcgcaa actcaccaaa caagaagcca ccgccctcag ttggcatctc catgatcaac
      181 tccccctgct catcgccacc ggagccaatt ctcccatatg ggacaaaaaa gtcacaggga
      241 aagcctccaa caggtttctt cgtggaaatg ccacctactt cacgcccacg aaacgagggg
      301 atctcacttc ggtcgatacc agggaactgg tctatagcaa aggacgaaaa actaaaccgc
      361 ccacgctgga aatccgggtc ttcgattcca atattccgga atttgtcgtg gcgaatttat
      421 gtctggtgaa agccatatgt ttgcgatggc tcagagggaa ggcggctgcc aatcgaatga
      481 gccatgccga ttacctcctc gccagaacag aagccgcgac caaaggcatg aaagccaaca
      541 tcccctggaa acgggaatgg gtaccagctc cggaatattt ggaccgattc ctctgggaac
      601 accgggaaga aatcgatgac atggatattc ctgaagaaat ctatgaggtt ctccggctgt
      661 tcaaacgcag atataacggc acgcgactca ttcatgatgc agtcgccctg gccattcgtg
      721 agcacccgca aacctggcaa cggcggtttg ccaaacgcta tcgctcagga ttaggccatt
      781 tgctcagtgg gaacaccctg caggattttg ccaccgaatt aggggtcccc tttcccagta
      841 ccgaacgggt gtggctgggc cggaagcgag cctccatcga tgagtaaacg ttttcccgcc
      901 ccgttaatcg gcatcacctg tgaagccgta tccaaccgca aagacttcgc caactacgat
      961 ttattatgtg accaccgcta tgcggccgcg gtcaccgaag cgggcggcca tccggtgttg
     1021 ctcccgatta atcacaggga aaccctacgc aaccgttatc tcgaaggcat tgacgggttg
     1081 gtcattgtcg gaggcgacga tctggaccca agctggtatg gagaacgtcc taaaaaacgc
     1141 acaaaagtgg ccttcaaaaa ccggtcaaag tttgaagcct ggctctacaa ggctggaaaa
     1201 actcggcggt tgcctatttt gggaatctgc tatggcatgc aattgatcaa tgtcctggaa
     1261 ggaggaaccc ttcatcaaca tattcatccc ccaaaacagg gctatcatgt ggatcatgaa
     1321 ggcaagaaaa acgggctaca tcccatcaag gtcattcagg gcacccgatt ggcgacaatc
     1381 ctcggaaagg gtcgaaaggt ggtcgccacc gaacatcatc agggcgtccg gaccctcgcc
     1441 cccgatttca ttcctgccgc catcgccagc gacggactga ttgaagcgat ggaacatccc
     1501 aggtaccccc atatttttac cgtgcaatgg catcccgaac gacaactacg aagccaaacc
     1561 acacaaaagc tcctgcgggc gttcgtccga tcctgccaac actaccaacg aacaagaaac
     1621 ccgtaatcgg ccaggttcat caccaatcgt caaacacaca gattctacaa tacaacagac
     1681 aatgagcccg aaataagatg gtcaaacgct atcgcgtggt aatctaagag gccccaaccc
     1741 gacatgaaac attggtaaga tgatccgata agcccgtgct acatggtcat tctgagccgg
     1801 aggcggaaga atctgtgccc aggtcgatta gtcccatgcc tcagcgagat ccttcgattc
     1861 tatcaggctg acaagaacgt tgctgaatag aaccatggga cacacgtctt caccacatcc
     1921 aatcagccat gtggcaaatg gaattcagac gattctttcc ggtataaaaa agataactcg
     1981 aatattcaaa acatgatttc ctggcaccat ccctggaaac tccaagccaa ggcctccctc
     2041 atcatcatcc tcattgtggg cggggccatt ctggtcacag aactcttgca tcaatattat
     2101 gtggcggaat tggccaaaga tgtcattcac tccaagtctc tcgtcctcct tcgccaaatc
     2161 ggcgcacggt ttcaatcaga aaagcatttt cacaatgcga cattgcggga gaaatactta
     2221 aacgatttga tgagccggaa tcccgatctc atcttcctcc gcgtctatgg gaaaggcacc
     2281 aaggccgatg atccgccgat cctgctcacg caagttggga acaccgagga cacttcatcc
     2341 gaaattccgt tgatggtcac ccacaccttc caatttgaag aaccccaagg ccgctttcac
     2401 aatcaacctg gcgatgatcg gctcaaatta tccacgcctc ttgccataca ggggaaaacc
     2461 accggggtca tctatgccga atactggacc gggcaactca ccaccatcac gcgatttttc
     2521 ctcaattggt cactcatcat cagtctgatc atgggattcg gcatcgtggt ggcgttaaac
     2581 ttctttctct atcgacatgt gatccgtccc cttgggcggc tcaaaaccgg actggctgaa
     2641 gtcgaaaagg gccaatgggc cactttggtg tcgatcgaaa aaacagacga aataggcgat
     2701 ttagcccgcc agttcaattc aatggcacaa cagatccaag aggtcatgag ggaaaatgcc
     2761 tcattgaatc aggcgttagc ccaggcacgg gatgtcctac aaaccagagt ggaggatgca
     2821 acagcagaac tccggcaacg gaacgaagag ttggccgccg tcaacgagcg cctctccatt
     2881 gcgcaacgtg acattctccg gcaacaacgg ttagccgtcc tgggtcaact ggttgccacc
     2941 attgcccaca agatcggcac acctttaaca gccatcttcg gacacctcca acttctccag
     3001 gaagatccac atcttcccca ggaagtcctg gaacgagtgc gaaccatgct caaacaaacc
     3061 gatcgcctca cccacagcat ccaggactta ctcaccttca cccgaacacc caccatctcc
     3121 gtcgaacagg tctccctccc gaccctgatc gaccagtcac tgttattatt tcgcccgctt
     3181 ctccaagagc acggcattca agggctcacc cacttttcgc cggatctgtg gccaattcag
     3241 ggagatgcct ttcatcttca ggaggccatc aacaacctca tcgataacgc catcgatgcc
     3301 atgccggacg gcgggcattt gaccattcag gccctaaacg ccgaagatac ccctcacgcg
     3361 ggaccggagg taaccgtcga cattcacgat tcgggaccgg ggattccgcg ggaacacatc
     3421 gaaaaaatct ttgacccctt ctttaccacc aaaagtgtgg gatcaggaac gggattgggg
     3481 ttagccatta ccaccgaaat tattcaatta cataagggcc acatcaccgt gcagagcgaa
     3541 gagggccagg gcacccgctt tctcattcgg ttgcccgcct ggaggatccc ggatcatgca
     3601 taaagccacc attctcatcg tcgatgatga accggacacg ctgactctgc ttcgggaaat
     3661 catggaaaaa gaaggctatc aggtgcacac cgccacgagt gggcaagccg ccttacacac
     3721 cttcgtcgat caaacgccgg atgtggtgtt atcggatatt caaatgccac acatggacgg
     3781 gttggccctc ctggccgaaa cacgaaaacg cagccccctc acccaggtca ttctcctcac
     3841 cgcctatggg tcgttgtcca cggctgtgga aggcatcaaa gccggagcct tcgattacct
     3901 ctccaaaccg ttcgcattgg aagaaattcg ttccgtcgtc cgccgggcct gtgaccataa
     3961 acacaccctg gaagaggctc gggtatcgat tcagccactc ctgccaaaag gaaatggcat
     4021 caaccagata caaggcaaca gtccgccgat ggtggccgta tacaaactca tcgcccgcgt
     4081 cgcaccgacg gaatccaccg tcctcatcca tggtgaaacg ggaacaggaa aagaactcat
     4141 tgcccgtgcc atgcatgcca acagtctgcg aaacgacggg ccgtttatcg ccgtcgattg
     4201 cggcacgctc acagaaaatc ttctggaaag cgaattattc ggtcacgaac gcggcgcctt
     4261 cacgggagcc atcacactca aaaaaggact cctggaatcg gccaatcgag gcacctgttt
     4321 cctggatgaa atcggcgata tctccccgaa cctgcagagc aaacttctcc gggtcctgca
     4381 agagcatgaa attcgccggg tgggcggcat ggaatcgatc aaagtcgatg tccgcatcat
     4441 tgccgccacc aacaaacacc tcaaaaaact cgtggaatcc gggaagttcc gggaagatct
     4501 ctattaccgc ctcaacgtcg tcacactgca cctgccctcg ctacgggaac gggtcgaaga
     4561 catccctaca ctcatagact acttcctcac caaatatacg cagctcaaca ataaggtcat
     4621 caccggagtg catccgcagg cgttaaccct gctcaccaac tattcatggc cgggaaatgt
     4681 gcgtgaactc gaacatacca ttgaacgagc cgtcgtcatg actccacatt ccctcatcat
     4741 gtctcacgac ctcccgttag cactcgtcgc gcctcccgcc ctccccgacc aatcggcatc
     4801 tcatccagat tggaaaacgc tcggacagct cgaacgggaa cacatcctca aagtcttaga
     4861 tgctcagcaa ggcgacgaaa accgcacctc ggaactcctt ggcatccacc gaaaaaccct
     4921 ccagcgcaaa ctcaaagaat acggcctccg ctaacctcaa tccgccctcc ccgtcatcag
     4981 catccgatat ccacctaggt ttctcaccat aagaaaagga aaccagcagg aagcaaaaaa
     5041 tataaagaaa cttcactgac ctatcacctt caggtcagca aactggcctg acgtttgctg
     5101 gtgaccctac ttcacccttt ccttgctcga gatcataggt ggtaagttca gtccttgcct
     5161 tcacacgtac tcccaaaccc cttcccacct tcctgctgat tcagaatggt ttccttttct
     5221 ttcttggttc ttccggggga aaagattgag cccttcggca tgcctcaggc caggcatcgc
     5281 gagtttggtc gccccacgtt
//
LOCUS       DAORAQ010000171         5282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2254_length_5282_cov_71.543715, whole genome shotgun sequence.
ACCESSION   DAORAQ010000171 DAORAQ010000000
VERSION     DAORAQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2254_length_5282_cov_71.543715"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PKM72_11875"
     CDS             complement(<1..166)
                     /locus_tag="PKM72_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNP61532.1"
                     /translation="MIPADVKLDTLGYFCPMPIILTSKKIKELASGQVLEVISDDEGI
                     KKDMPAWCETT"
     gene            complement(190..720)
                     /locus_tag="PKM72_11880"
     CDS             complement(190..720)
                     /locus_tag="PKM72_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNP61533.1"
                     /translation="MNATHVEPATALAQLKETKPDKVTLVVLSGDMDRVMAALIIATG
                     AAAMGMQVTMFFTFWGLNAIRKENMSSSPKDWLRRAFGWLNKGGASRLPLSRFHFGGL
                     GTTMMKKVMKDNRMPGIPELMETAKDLDVKMIACTTTLGLMGISKDTLIDGIDQLAGV
                     STYLAEARHGSVNLFI"
     gene            983..1900
                     /gene="ftcD"
                     /locus_tag="PKM72_11885"
     CDS             983..1900
                     /gene="ftcD"
                     /locus_tag="PKM72_11885"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNP61534.1"
                     /translation="MDRLVECVPNVSEGRDTALLALLTEGIRSVPNVSLLDLHVDSDH
                     HRSVFTLAGEPEAMATALFQFIREAQHGIDLRTHQGQHPRIGAVDVVPWIPLRGVTME
                     ECAGYAKDLGRRVGQELGIPVFLYEQAAIVPLRARLDVIRRGDLSGLQERMYQEADWK
                     PDFGPNVLHPTAGAVAIGARFFLIAFNVVLKSQDIQVARRIARAIRSSGGGLPALKAM
                     GVPLPSKGLVQVSMNLIDFRQTSLRAAFHAVERESHRWGVDIEESEIVGLVPQEAWDV
                     DLSTDLKLKDWNPERVLEVACGKYGLFPL"
     gene            2033..2461
                     /locus_tag="PKM72_11890"
     CDS             2033..2461
                     /locus_tag="PKM72_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP61535.1"
                     /translation="MSDDVAALRASKFGQLTVGSVMEKEVQSGMKDTEAKLLASYMME
                     GFGSVPIIDETSKLVGIVSEFDLLKALRKGKNLEDVTAGDIMTANPVSVTQDTNVLTL
                     MDVLQNNHLIRVPVVDSKGKLIGIVARRDLLRGYLQTSGS"
     gene            2597..3436
                     /locus_tag="PKM72_11895"
     CDS             2597..3436
                     /locus_tag="PKM72_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNP61536.1"
                     /translation="MKDRAKQIVHAPWFEYGIIAFILINGVILGLETSSALVEQYGAL
                     MHVGNHVILGIFILEALIKMIAVAPQIDRYFRDGWNIFDFSVIVFSLIPATGEFAMIA
                     RLARLLRVVRLISTIPELRLIVSTLVRSIPSMIHVMTLMGVIFYVYAIMGYQLFHEHD
                     PTHWRSLGISLLTLFRVVTLEDWTDVMYTAMDFHYLSWIYFVSFVVLGTFVVINLFIA
                     VVINNLDEAKAERLAELQGPVTQKEILKDLRETQIALKRLEERLERTSGENVLPLSKV
                     LKG"
     gene            complement(3460..3693)
                     /locus_tag="PKM72_11900"
     CDS             complement(3460..3693)
                     /locus_tag="PKM72_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61537.1"
                     /translation="MKIETPVAAAAVKETGFDNKVLPDGQTTLSEVEGILSPSNQRQL
                     PESNDFVPDIYKRHTLLACAPDTGLAVMKKESK"
     gene            4615..>5282
                     /locus_tag="PKM72_11905"
     CDS             4615..>5282
                     /locus_tag="PKM72_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61538.1"
                     /translation="MKLNGLFKIVVILGMVAINSSAWGHSPNDAAEGGLQSKVRESGL
                     IMYGQVVDIQYRNSEPTKEQPQGLPHTFVSYQVLEVLRGDVPDKKVTLRIPGGADGQG
                     GVYMVSTAPAFALGQTDVLFVKGGEIDDCQLVECVEGRFRIHENQVLNGWGVPVVEAH
                     KNLRIGGKPRFDLNVMEIPRPSFKALLERPDMKAYIDRVSRESRQSLKDLQARYEREA
                     PKVTT"
BASE COUNT         1256 a         1154 c         1398 g         1474 t
ORIGIN      
        1 cggttgtttc gcaccaggcc ggcatatctt ttttaatccc ttcgtcatcc gaaataactt
       61 caaggacttg ccctgaggcg agttctttga ttttctttga ggtcagaatg atcggcattg
      121 ggcagaaata ccccaatgta tcgagtttaa cgtcggctgg tatcatggtt tatccttttg
      181 tggtggtcgt tagataaata agttcacgga tccgtgccgg gcttccgcca gataggtgct
      241 gactccggcg agttggtcga tgccgtcaat gagcgtatcc tttgaaatgc ccatgagtcc
      301 cagggttgtg gtgcaggcaa tcatctttac atccaggtct ttggcggttt ccatgagttc
      361 agggatgccg ggcatgcgat tatctttcat gacctttttc atcatggtgg ttccgagacc
      421 tccaaaatga aatcgtgaga ggggcagacg gcttgctccc cccttgttca gccatccaaa
      481 ggctcgacgg agccaatcct ttggagagct actcatattt tcctttcgga tggcattcaa
      541 cccccaaaag gtaaaaaaca tggtcacttg catgcccatg gctgcagcgc ccgtggcgat
      601 aatcagggcc gccatgacac ggtccatgtc tccactcagg acaacaaggg tcactttatc
      661 gggtttggtt tctttgagtt gggctaaggc ggttgctggt tcaacgtggg tggcgttcat
      721 gatgtcacct tctttctcac aaaattatgg gtcgcatctt acagagtctt cgataaaggc
      781 ggattgttac ggagtatagc agttcttggc ctttgatggg gttgattctg ccgggatgca
      841 ttatccacgg ctgaggctta cagctgattc gactataata tggtaaattt ttcggtgtca
      901 acaaaagcct gttctggatt gagcctccat gaggcatgaa actcaggctc ttagccgtgt
      961 aggcagatgt agggaaatgg acatggatcg cttggtagaa tgtgtgccga atgtcagtga
     1021 gggccgggac acggccctac ttgctttatt gactgagggt ataaggtcag tacccaatgt
     1081 gtccctactc gatcttcatg tggattcaga tcaccatcgg tctgttttta cccttgcagg
     1141 ggagcctgag gcgatggcca ctgcgctttt tcagtttatt cgagaagccc agcatggcat
     1201 cgatctccgc acgcatcagg ggcagcatcc acgcatcggg gcggttgatg tggtgccctg
     1261 gattccctta cgaggcgtga cgatggagga atgtgcaggc tatgcaaaag atttgggccg
     1321 gcgagtcggg caggaactgg gcattcccgt ctttctctat gaacaagcag ctatcgtgcc
     1381 tttgcgggcc aggttggacg tgatacggcg gggagatctc tcggggttac aggaacggat
     1441 gtatcaagaa gcagattgga aacctgattt tggcccaaat gttttgcatc ctactgcggg
     1501 agccgtcgca atcggagccc gttttttctt aattgccttt aacgtggtgc tcaagagtca
     1561 ggatattcag gtggctcgtc gtattgctcg ggcgatccgg tcttcagggg gaggcttgcc
     1621 tgccctcaag gccatgggtg tgccattgcc atcaaaaggg cttgtgcagg tgtccatgaa
     1681 tctgattgat tttcgacaaa cctccttgcg agcggcattt catgccgtgg agcgggagtc
     1741 ccatcggtgg ggtgtggaca ttgaggaaag tgagattgtg ggcctggtgc cgcaggaggc
     1801 atgggatgtg gatctgtcta ctgatcttaa gttgaaggat tggaaccctg aaagagtgtt
     1861 ggaagtggcg tgtggaaaat atggcctttt ccctttgtaa atcatcaggt acaataaccg
     1921 gaaaatgtga agatgaattc caagtcccgc tgtttttgag cgggggcact gaaactattc
     1981 aaaaaagtaa cgaagtgggg catgaccttc tactcaaaaa ggaataaacc atatgagcga
     2041 tgatgtcgca gctttaaggg ccagtaagtt tgggcagtta accgtgggca gtgtcatgga
     2101 aaaagaagtg caatcgggca tgaaggatac ggaagccaag ttgctggctt cttatatgat
     2161 ggaaggattt ggttcggttc ccattatcga tgaaacctcg aaattggtgg gaattgtttc
     2221 cgagtttgat ttacttaagg ccttgcgcaa agggaaaaat ctggaagacg tgacggcagg
     2281 tgatatcatg actgccaacc cggtgtcggt cacgcaggat actaatgtgc tgacgcttat
     2341 ggatgtcctg caaaataatc atttgattcg ggtgcctgtc gtggattcga aggggaaatt
     2401 gatcgggatc gtggcgcgaa gagatttgct tcggggatat ttgcaaacat cggggagttg
     2461 aagctccccg aggagttgag ctactccttt ccttcactgt gctcattcct cccattttcc
     2521 aatactaatg aatcggtgct cgctctcttc ctgattccag gtcgagaatg tgccataagt
     2581 ttgtgagtta acgacgatga aagatcgagc gaagcagatt gtccacgccc catggtttga
     2641 gtatgggatc attgccttta ttctcattaa cggggtcatt ttgggcctgg aaacctcatc
     2701 agcattggtg gaacagtatg gtgccctgat gcatgtgggt aatcacgtta ttcttgggat
     2761 tttcattctt gaagccctca tcaaaatgat tgccgtggca cctcagattg atcgatattt
     2821 tcgagatggc tggaacatct ttgatttttc cgtcatcgtg ttttctttga ttccggctac
     2881 cggtgaattt gccatgattg cccggttagc gcgcttgttg cgggtggttc gtctcatctc
     2941 gaccatacct gaactccgcc ttatcgtttc aaccctcgtc agatctattc ccagcatgat
     3001 tcacgtcatg actctcatgg gcgtgatctt ttatgtctat gccatcatgg gctatcagtt
     3061 gtttcatgag cacgatccca cgcattggcg atcattgggg atctcgctgt tgaccctctt
     3121 tcgcgtcgtg accctggaag attggacgga tgtaatgtat accgccatgg attttcatta
     3181 tctgtcctgg atctattttg tgagttttgt ggtcttaggg acgtttgtcg tgattaatct
     3241 atttattgcc gtggtgatta acaatcttga tgaggccaaa gccgaacggc tggctgaact
     3301 tcagggaccg gtcacacaaa aagagatact caaagatttg cgagaaacac aaatagccct
     3361 caagcgccta gaggaacgat tggaaagaac atctggagaa aatgtcttac ccctatcgaa
     3421 agtgttgaaa ggttgaattg ccaagaaata tagtgttatt catttgctct cttttttcat
     3481 gaccgccaat ccggtatcag gcgcgcaagc taacaaggtg tgtcgcttat aaatgtccgg
     3541 aacaaaatca ttggattcgg gtagctgtcg ttgattcgaa ggggaaagga tcccctccac
     3601 ctcagagaga gtcgtttgac catccggcag gaccttgttg tcaaatcccg tttccttcac
     3661 ggctgcagcg gctactggag tttcgatttt caatgatttc gatccaaatc tctccggtcg
     3721 atagacgtaa gatcctgata aattccttcc cgtcttccca gcgagagaga atttgaaaat
     3781 cagaatgtac attgagtgcc atatggatgc cattgggaca ttctattaac gtctgacttc
     3841 cctatccggt tttgacctta tcccctccat aaaagggttg gatgtcttac ctctccagtt
     3901 gatcctgaat accatcagtt cgaaatactc tgaattcgcc tatggctttc ctcattctca
     3961 tcttcagcga actactcccg ccgtcgttcc ttatgtaagg ccaaagctgt acaacagttt
     4021 cgatcctgct cccctcttgc gttggtattg attccaatct catttaacgg tcagtaacgt
     4081 gttctctgtg ctctagagct gggccatctc gattttgcct catcgtttgg gcattggctt
     4141 atatggctct atggccttcc tgtgtcgatg gaggcatgga aatggatagg caaaccgttt
     4201 tcgatgaggg aaaaaggcgg gcggaaggat cgttatttaa aaactcggtg tgcgtatctg
     4261 aacagatacg attcgtatct catcttgttt tctttccacg ataaagctga gttgatcgaa
     4321 caacatctgg atcgaaatgt actcaaattg cctatgaata tgggagattt tcgctagcgg
     4381 aagtaatttt cgttcgctga agcttttggc attgaggttg ctatagcaag ctgcagaatg
     4441 atttttttac attaactcac caaggagggt gcatggccag aaagcagatg aacaagattc
     4501 agatggtgcg aatgaaatgg acacacgata tcttcgggga tccataaatg aagattaatt
     4561 cgtcacgatt ccggcaattg tgtattcacg caaatgaaag agaaagggca ttctatgaaa
     4621 ctcaatgggt tgttcaaaat agtcgtaatc cttggcatgg tggccatcaa ttcatcagca
     4681 tggggacata gtccaaacga tgccgccgaa ggcggccttc aatcgaaagt tcgtgagtct
     4741 ggtctgatta tgtatggcca ggtggtggat atccaatatc gaaattcaga accgacgaaa
     4801 gagcaaccac agggccttcc tcatacgttc gtgagttatc aggttttgga agtgttgaga
     4861 ggtgatgtcc cggacaaaaa agtcacgctc cgaattcccg gtggggccga tgggcaggga
     4921 ggtgtctata tggtttcgac agctcccgcg tttgcccttg gacaaaccga tgtgttgttc
     4981 gtcaaaggag gagaaatcga cgactgccag cttgtggaat gtgtcgaggg aagatttcgt
     5041 attcatgaaa atcaggtgtt gaatggctgg ggagtgccgg ttgtggaagc ccacaaaaat
     5101 ttacgcattg gaggaaaacc acggttcgat ctcaatgtca tggaaatacc tcgcccctcg
     5161 tttaaagccc tgctggaacg ccctgatatg aaggcttata tcgatcgagt gagccgggag
     5221 agccgacaga gcttgaaaga tctgcaggcc cgctatgagc gggaagctcc aaaagtcacg
     5281 ac
//
LOCUS       DAORAQ010000172         5225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2280_length_5225_cov_70.471373, whole genome shotgun sequence.
ACCESSION   DAORAQ010000172 DAORAQ010000000
VERSION     DAORAQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2280_length_5225_cov_70.471373"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1518
                     /locus_tag="PKM72_11910"
     CDS             91..1518
                     /locus_tag="PKM72_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNP61539.1"
                     /translation="MDLTPIPVKQTPSQVVFPHKVHLPRVPDRHTHFESWAAVKIPKK
                     LYTGTRILIGFFLVFILILVFVPWTQTIKAQGQLSAYSPAERPQEIHAPLEGRILTWH
                     VNEGMAVKKQDLLLDLVDVDSKFLAPDLLTRLDESIQALEERRETTLARADFLGRQLK
                     EMTPLTEATTRSAESRVQEASRRIQSMVQRQAAAKVAAQTAQLNLQRSQILEAEGLLS
                     RRELELAIQAEAAAQAERKASDAAFQEVSQSRLALTHGRVQIEAELGQRILNIRSQRA
                     AALGEAAQASKELADLALTRSNAAQRRTASRITAPMDGTVVRMARVGAGEIVKQGDSL
                     FTIVPDTATRAVEMWAEPLDAPLLRPGRKVRLMFHGIPTIPLPSWPELMAGTFDGTIL
                     VVDQTPDSQKRFRFWVVQDPEAAAWPPQSQVRQGTQVMGWVLLNRVPLWYELWRRVNL
                     FPADYQGQSTYLPETFLPKAGRPGK"
     gene            1580..3025
                     /locus_tag="PKM72_11915"
     CDS             1580..3025
                     /locus_tag="PKM72_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP61540.1"
                     /translation="MPRNLMTLSCLFAGAVLVWSPLPDTFALETTTSRYDAVTRSLRL
                     ETVLSLVETQHPLLHGSRTEKLEAQGKLLKALGAFEPILVNDLEVERLVKDGKTKSVG
                     FNDTMIQMRHPWGLQGFAGWRTGLGDVEVADLGVNQTNQPLLGLVLPLLRGLGTNPEK
                     GELEKSKLAEQEATLHIQQTRQDLYLGAATQYWRWVAAWKAVDLRQQALEVAKVRLQQ
                     LTRQAEAGAVAEFDVTEAHQEVQRRLENVIKTKREAEQEQFKLSLFVWDHGSPMVFSL
                     QSVPDFPSPTSTSGNQSRETDVDLAQQRRPEILQITLEAARNHIDLGVAKNNLLPDLR
                     AEAEPTRKPGEFVLGLGYRFGLQLSFPFLQKDATGQQMQMKAKAERLLWDQQYRMQQV
                     ALDIDNANSALLRAEERMQAASQALIYARSLVKGERTRFQVGATNLLFVNLRERNVVD
                     AEVIYIQAQAEYHQAQAFYQWATGHWTQPVA"
     gene            3080..4708
                     /locus_tag="PKM72_11920"
     CDS             3080..4708
                     /locus_tag="PKM72_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNP61541.1"
                     /translation="MNKKVLQHVGVFLKQERQLLLTILMYSLAVGAFSLIIPLTVQEL
                     VNTFALAIQPIMVVTLVSIMAGVLAFVGVFKVLQFYATDLLERRIFVRMTLAFARTFP
                     SIQETHFRGEYASRFFEIVFMQRAVAGLLVDLTNVVVSGFIGMTLLVLYHPYFLVFDL
                     LLLLAVGIIGVLGQGGLHTTLHMSETKYATYHWFQEVADNLSHFKSSQSRDLILQKAD
                     SLAHAYVHARESRLRVLIRQYIGSISLQVLLHTGLLGTAGWLLSNGELTLGQLVAAEV
                     IIATLLVNLDSVVKRTYVIFYFLTALTEIQHIFSLPKDHQTSTQFVSLPEVSPKGIGL
                     SISHVGGLPPPWPHAFELTIDLKPGEKWALVTVTESQRLKVSRLLAGLDDPPHGTIRY
                     NGIDIRDLHPDAVNGIRGLVLSRDLSLFEATFLENITLGRKDLSTEDLLWVLNFVDLQ
                     KETETFPEGLQTMVQYGGKNFSPSQTIQILLARALIARPKLLVVDGGLHEIPSPQRET
                     ILGRICAAESPWTVVIVTTDPNVKTFVQQSVSLH"
BASE COUNT         1255 a         1333 c         1333 g         1304 t
ORIGIN      
        1 aggaataaca ccattagcag attcacccat tctcaatgtt tcggctaagt aagaatgatg
       61 actcgaaagt cctcagtaag cacgtaactc atggacctta cccccattcc agttaaacag
      121 actccctctc aagttgtttt tccgcacaag gttcacctgc ctcgtgtccc tgaccgtcat
      181 acgcattttg aatcgtgggc agccgtgaag attcccaaga aactgtatac cggaacccga
      241 atcctcatcg gtttttttct tgtattcatc ctgattctgg tttttgtccc ctggactcaa
      301 accatcaagg ctcaagggca gttatcggcc tattctcctg ctgaacgccc gcaggagatt
      361 catgcacctc ttgaaggccg tatcctgact tggcatgtca acgaaggcat ggcggtgaaa
      421 aaacaggatt tgctccttga tctcgtggac gtcgattcca aattcctggc cccggatctc
      481 ctcacacggc tggacgaatc cattcaggcc ctggaagaaa ggcgggagac cacgttggct
      541 cgtgccgatt tcttaggcag acaactcaaa gaaatgaccc cactgaccga agccaccact
      601 cgatctgccg aatcccgtgt ccaggaagct tcaaggcgaa tccaaagtat ggttcagcgc
      661 caggctgccg cgaaggtcgc cgcccaaact gctcaactga atttgcaacg ttcacaaatc
      721 ctggaagccg aagggcttct ttctcggcga gaactggaat tagccattca agctgaagcc
      781 gccgctcaag ccgaacgcaa agccagtgac gccgcatttc aagaagtgtc tcaatcacga
      841 ctggccctca cccatggacg agtccaaatt gaagccgaac ttggccaacg cattctgaac
      901 attcgttccc aaagggctgc agccctggga gaggctgctc aggcgtcgaa ggagttggcg
      961 gacctggctt taacccgctc caatgcagcc cagcgacgaa cagccagccg tatcaccgcg
     1021 cccatggatg gcacagtggt ccgcatggca cgagtgggag caggtgaaat tgtgaagcaa
     1081 ggggattcgt tattcaccat cgtcccggat acggccaccc gggccgtgga aatgtgggca
     1141 gaaccgttgg atgcgcccct tctgaggcca ggaaggaagg tccgactgat gtttcatggc
     1201 attccgacta tcccacttcc ttcctggccg gagctaatgg ctggaacgtt cgatggcacc
     1261 attctggtgg ttgatcaaac cccggattca caaaaacgat ttcgcttttg ggttgttcaa
     1321 gatccggagg ccgctgcgtg gccaccgcaa agtcaggttc ggcaaggtac tcaggtcatg
     1381 ggatgggttc tgttaaaccg ggtacctctt tggtatgaat tgtggaggcg tgtgaatctc
     1441 ttcccggctg actatcaagg acaatcaacc tatcttcccg agacgtttct ccccaaggcc
     1501 ggacgtccgg ggaaataatt acggctccag cggatacacg gcagtcaatt ctgaaaacta
     1561 ttgtaaggag ggagaaggca tgccacgaaa tctcatgaca ctttcctgcc tgttcgcagg
     1621 cgcggttctt gtatggtctc ctttacccga tacattcgcg ctggagacca cgacttcccg
     1681 gtacgatgcg gtcacacgat cacttcgctt agaaaccgtg ctgtcgttgg ttgagacaca
     1741 acatccactg ttgcatggga gtcggactga aaaactggaa gcgcaaggga aattactgaa
     1801 agctcttggt gcatttgaac ccatcctggt caatgatttg gaggtagagc gactggtcaa
     1861 agacggcaaa accaaaagtg tggggttcaa cgacaccatg attcagatgc ggcatccctg
     1921 gggactccaa ggatttgccg gatggcgaac cggtctggga gatgtggagg tggctgatct
     1981 cggagttaac cagaccaatc aacctctgtt aggcctcgtg ttgcccttac tacgaggatt
     2041 gggcaccaat ccagaaaagg gggaactgga aaaatccaaa ctggcagaac aggaagccac
     2101 tcttcatatt caacaaaccc gccaagatct gtatttgggt gctgctaccc aatattggag
     2161 atgggtggcc gcctggaaag ctgtggattt gcgccaacaa gccttggaag tcgccaaggt
     2221 tcgccttcaa caactgaccc gacaggctga agccggtgcc gtcgcggaat ttgatgtcac
     2281 cgaagcccac caggaagtcc aacgccgact ggagaatgtc attaaaacca aacgggaagc
     2341 cgaacaggaa cagttcaagc tgtccctctt tgtctgggac catggttccc ctatggtctt
     2401 cagtttgcag tcggtccctg actttccttc tcccacttcg acttcgggta accaatctcg
     2461 ggaaactgat gttgatctgg cacaacaacg ccggccggaa attcttcaaa ttacacttga
     2521 ggcggcgcga aatcatattg atcttggtgt cgccaaaaat aatttgctcc ctgatcttcg
     2581 ggcagaggct gaaccaacca ggaaacccgg agaatttgtg ttgggtctgg gttaccgctt
     2641 cgggctccaa ctcagctttc cattcttaca aaaagatgca acaggccaac aaatgcaaat
     2701 gaaagccaag gcggaacgat tactgtggga tcagcaatac cgtatgcaac aagtggcgtt
     2761 ggatatcgac aatgccaact cggctttatt acgagcggaa gaacggatgc aggcggcttc
     2821 tcaagccctc atctacgccc gctctcttgt gaaaggcgaa agaacccggt ttcaggtggg
     2881 ggccactaat ttactttttg tgaatcttcg ggaacgaaat gtagtggatg cagaagtcat
     2941 ctatatccag gcacaagccg aatatcatca ggcccaagcc ttttatcaat gggccaccgg
     3001 ccattggacc cagcccgtgg catagccacc aacaagaacc ggcgtattct taatgatctc
     3061 ctccgagtct gtccggagca tgaacaaaaa agtcctccag catgtgggcg tttttctcaa
     3121 acaggagcgc caacttcttc tgacgattct catgtattcg ttggccgttg gtgcattttc
     3181 acttattatt ccgctcacgg tccaggagct ggtgaatacc tttgcgttgg ccatccaacc
     3241 gattatggtt gtcacgctgg tcagtattat ggccggggtc ctcgcatttg tcggagtgtt
     3301 caaggtcctt cagttttacg ccacagatct tcttgaacgg cgaatttttg ttcgcatgac
     3361 tctggccttt gcccgaacat ttccctctat acaagaaaca catttccgcg gcgaatatgc
     3421 cagccggttt ttcgaaattg tttttatgca acgggccgtt gccggtttat tggtagacct
     3481 taccaacgtg gtggtgagtg ggttcattgg catgacgttg cttgtcctgt atcacccgta
     3541 ctttctggtt tttgatctcc ttctcctcct ggctgtagga atcatcggag tattaggcca
     3601 agggggactt cacactaccc tgcacatgtc ggagaccaaa tatgccacct atcattggtt
     3661 tcaggaagtg gccgacaatc tcagtcactt taaatccagc cagagtcggg atctcatctt
     3721 acagaaagcc gattccctgg cccacgccta tgtccatgcg agagagtcca ggctcagggt
     3781 gctgataagg caatacattg ggtctatttc tcttcaagtc cttctgcata cgggtctgtt
     3841 ggggacagcg gggtggctgt tgagtaacgg ggagctgacc ttaggccaac tcgtggctgc
     3901 ggaagtcatc atcgcgacac ttcttgttaa tttggattcg gtcgttaagc gcacctatgt
     3961 gattttttat tttttaaccg cgttaaccga gattcaacat attttttctc ttcccaaaga
     4021 ccatcaaact tcaactcagt tcgtcagctt gcccgaggtt tcccccaaag ggatcgggct
     4081 ttctatctcc catgttggcg gtttgcctcc tccctggcca catgcctttg aactcaccat
     4141 agatctgaag cctggagaaa aatgggcatt ggtgaccgta acggaatccc aacgattaaa
     4201 ggtttctaga ctgttagcgg gtttggatga tccaccacac ggaactattc gctacaatgg
     4261 gattgatatt cgtgaccttc atcctgatgc cgtgaatggc attcgtggtc tggtgttgag
     4321 tagggatttg tcactttttg aagccacgtt cttggaaaac atcacattgg gacgaaagga
     4381 cctgtcaacc gaggatttac tgtgggtttt aaattttgtt gatttgcaga aagaaacgga
     4441 gactttccca gaaggtttgc aaacgatggt tcaatatggt gggaaaaatt tttcacccag
     4501 tcagactatt caaattttat tagcccgggc cttgatcgct cgcccgaaac tcctggtggt
     4561 cgatggtggg cttcatgaaa ttccttcacc tcaacgtgag accattcttg gcaggatctg
     4621 tgcggctgag agcccatgga cagtggtaat tgtcaccact gatcccaacg tcaaaacgtt
     4681 tgtccagcaa agtgtatcat tacactaacg cgtggttgag gaatgttgtt gaataaccgc
     4741 gaagcatgaa gcatgggcat cacaaacgaa tttgcctccg tatgtagagg tggcagccgg
     4801 ttctctagtt aatgaaccgc ttgataggcc cgggggactt ggaggtgggt gtggtggcag
     4861 tcggcgaaca tgcgccatgc cggtctcgtg ggtttccgta cctaagacga tagatttgcc
     4921 ggctattttt tggagtgaga ctaccagtta gacgagcccg tcaaatactg tacacgtgtg
     4981 gctggtggcg catgaaagcc gccagggccg gacagtttca ggtgatcttc aagtctccac
     5041 cccggcaggc ctccttgacc aactgaattg tcgaccatca tatcctgtca aggcaatcgt
     5101 gtcgacagac cggctagtca tgtctgggga aattcagagt tttggtttct ttgccacatt
     5161 tggcgtataa ggagaaggcg tgttcaatac atgttcggtg aaaatggtgg tattcatgcc
     5221 gaagc
//
LOCUS       DAORAQ010000173         5199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2293_length_5199_cov_67.927488, whole genome shotgun sequence.
ACCESSION   DAORAQ010000173 DAORAQ010000000
VERSION     DAORAQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2293_length_5199_cov_67.927488"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PKM72_11925"
     CDS             complement(<1..1426)
                     /locus_tag="PKM72_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNP61542.1"
                     /translation="MDSSHTHTLFPSFLRSSITQSPHFHLVCVLLLAAWLLGVEGGWL
                     LGPDLGALGLTDRDEGSNAEAAREMLETGDWISPTLNYEPRYAKPAFVYWLISGSYAL
                     FGMNEFAARLPSALSGLCLLFLQYVFIRKWAGPTVAIFASCMLLLNLEFLGINRMVLT
                     DPELVVFTTLAGYSFWHALHHEQTKRWLFAIFYLAMGCGMLVKGPVGIIIPLVGVIPY
                     LTLTRQWGIFWQRGFPLVGIVLVAAVAAPWYVMMFQIHGDAYWAAAQANTTGRFMNPM
                     EGHGGTLLFYLPVLLVGFFPWSAFLFSVLYDTLKRWKAFWQGQGLATQEQHLECFLSL
                     WVVGLLLFFTLSATRLPHYIYPLFPAAALLVALWWKRFLREDAPVGLIASTRVLIAVG
                     YLLGIAMMFVPAIFQLFMKQIAKEFPAAVNVDLAWLPSLVGLVMVVGTMLMRQCMQSD
                     AKRHLALWVGCGMMLIFTVLVARGG"
     gene            complement(1419..1775)
                     /locus_tag="PKM72_11930"
     CDS             complement(1419..1775)
                     /locus_tag="PKM72_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNP61543.1"
                     /translation="MDGTLLAIHSLLLTMFESFTNTLMAMSFEELLWIGFGFLGQGIF
                     FSRWLVQWVVSERNEESQIPMSFWYMSLVGSVIVLTYAIHIADLVFMAGQSVGTVVYV
                     RNIMLLHQANKRVVSG"
     gene            complement(1848..2609)
                     /locus_tag="PKM72_11935"
     CDS             complement(1848..2609)
                     /locus_tag="PKM72_11935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNP61544.1"
                     /translation="MSLHPPRHWISVVIPIKDERENIPLLASQLLKFFESRPESGAAA
                     FELVFVDDGSVDGSGPLLDQLAGQFPAVLVIHLDRNHGQTAAFDAGFREARGELVATM
                     DGDLQYDPNDFAKLLPFVERYDLVCGRRQARHDSLVRRWSSKIANQVRNWAVHDGISD
                     TGCSLKVFRQAVVKRLPPFKNMHRFYPALAQMYGFTVTEIPVQHFPRAHGTSKYGIGN
                     RLFAGLYDLFAVRWMQKRCLNYTKKGIHPPRKSEL"
     gene            2848..3102
                     /locus_tag="PKM72_11940"
     CDS             2848..3102
                     /locus_tag="PKM72_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61545.1"
                     /translation="MQEEILQAYLEIEQAMQRYSMLLQDHVSQLGTQTDEESKNRFHR
                     MKAGSKAMRDSSQIYLSYAKYVAYGMPEGEELAEEEDLQA"
     gene            complement(3185..3261)
                     /locus_tag="PKM72_11945"
     tRNA            complement(3185..3261)
                     /locus_tag="PKM72_11945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3225..3227),aa:Pro,seq:ggg)
     gene            complement(3310..3660)
                     /locus_tag="PKM72_11950"
     CDS             complement(3310..3660)
                     /locus_tag="PKM72_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNP61546.1"
                     /translation="MGGGTKHEDKAFYKIGEVADLTNLPAYVLRFWESEFIFLKPKKS
                     QGKHRVYSKNDIETVFEIKRMLYDEGYTIAGLKRYWYRKKRSEKTAQVPKDRVEKAKV
                     ELQGILKILGRATM"
     gene            complement(3728..4003)
                     /locus_tag="PKM72_11955"
     CDS             complement(3728..4003)
                     /locus_tag="PKM72_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNP61547.1"
                     /translation="MRKADIAEIITETAAIQKTDSMEMVEHVLDLIKSMLQKGEVVKI
                     AGFGNFVVRNKGERKGRNPRTGEEIAITPRRVVTFRPSQLFKKYVNT"
     gene            complement(4171..>5199)
                     /gene="cimA"
                     /locus_tag="PKM72_11960"
     CDS             complement(4171..>5199)
                     /gene="cimA"
                     /locus_tag="PKM72_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNP61548.1"
                     /translation="MPWDIGEIFAKVRKNCSVPLGIHAHNDAEMGVANSLTAVQAGAI
                     QVQGTINGIGERCGNANLCSVMANLELKMERTALGDGRLAQLRTISNYVAEMANLTPN
                     KRQPYVGDTAFAHKGGVHIHAVQKNPLTYEHVNPDVVGNHRRVLISDSSGRSAVFGKM
                     ETFGLDLPQNNPKVLELLDSLKTLEYEGYQFEGAEGSFELLVRKAMGTYTPSFELLGC
                     RIIVEKRKTKEDPISEATIMVKVGEVVEHTAAVGDGPVNALDHALRKALEHFYPQLQE
                     MKLLDYKVRVLTGRHGTGSRVRVLIESGDHKEKWGTVGVSENIIEASWQALADSIEFK
                     LMKEKPQT"
BASE COUNT         1366 a         1450 c         1186 g         1197 t
ORIGIN      
        1 gtcctcctcg tgcgaccaat accgtaaaga tcagcatcat cccgcatccc acccataaag
       61 ccagatggcg tttggcgtca gattgcatgc actgacgcat gagcatggtt cccaccacca
      121 tgacgaggcc taccagtgag ggaagccacg ccaggtctac gttgaccgca gcgggaaatt
      181 ctttggcaat ttgcttcatg aacaactgaa aaatcgccgg cacaaacatc atcgcgatac
      241 ccaggaggta tcccaccgca atgaggactc gagtggaagc gatcagtcca accggcgcgt
      301 cctctctcaa gaaacgcttc caccacagag cgaccagcaa ggctgccgcc ggaaacagag
      361 gatagatgta atggggcagg cgcgtggccg agagggtaaa gaacagcaac aatcccacca
      421 cccagaggct caggaaacac tccaaatgtt gctcttgcgt ggcaaggcct tgtccctgcc
      481 agaatgcctt ccagcgtttt aacgtgtcat acagtacgga aaagagaaag gcactccatg
      541 ggaaaaaccc aaccaacaac accggcagat aaaataagag cgtcccccca tgaccttcca
      601 taggattcat gaaccgtccg gtggtattgg cctgtgccgc ggcccaatag gcatccccat
      661 gtatttgaaa catcatcaca taccagggag ccgcgacagc ggcaactaac acgatcccga
      721 caaggggaaa gccccgttgc cagaatattc cccattggcg cgtcaacgtc agatagggaa
      781 tcacccccac cagcggaatg ataatgccca cgggcccctt gaccaacatg ccgcatccca
      841 tagccagata aaagatggcg aacagccagc gcttggtctg ttcatgatgg agcgcgtgcc
      901 aaaagctata gccggccaat gtggtgaaca cgaccagttc cggatcagtc agcaccatgc
      961 gattgatgcc caaaaactcc aaattcagca gcaacatgca cgacgcaaaa atggccacgg
     1021 tggggccagc ccattttcga ataaacacat attgaaggaa caggaggcag agacctgata
     1081 aagccgaagg caaccgcgcg gcaaactcat tcatgccaaa cagggcatag gacccgctga
     1141 tgagccaata cacaaaagcc ggtttggcat agcgaggttc atagttgaga gtcggggaaa
     1201 tccaatcacc ggtctccagc atttcccttg ccgcttccgc attgctcccc tcatcccggt
     1261 ccgtcaaccc gagggccccc agatccggac caaggagcca tccgccctct actcccaata
     1321 gccaggcagc caggagcaat acacacacca gatggaaatg aggcgattgc gttattgaag
     1381 atcgaagaaa ggacggaaag agagtgtgag tgtgagagct atccactcac gactcgctta
     1441 ttggcctgat ggagcagcat gatattgcgg acatacacca cggttccgac gctttggccg
     1501 gccatgaata ccaggtcggc gatgtgaatg gcataggtca gcacaatcac actcccaacc
     1561 agactcatgt accagaatga catgggaatt tggctttcct cgttgcgttc cgacaccacc
     1621 cattgcacca accagcgtga gaaaaaaatg ccctgcccca aaaacccaaa cccgatccag
     1681 aggagttcct caaacgacat ggccatgagt gtatttgtga atgattcgaa catcgtcagc
     1741 agcagcgaat gaattgccaa aagggttcca tccattcaga accctttaat cgcatgttag
     1801 ttttgtggtg cctcaattgt ttccagcaga tggtcacttc aagaatgcta cagctcggat
     1861 ttccggggag gatggatccc ctttttggtg tagttcaaac agcgtttttg catccacctg
     1921 actgcgaaga gatcatacag accggcaaac agcctgttgc caattccgta ttttgatgtc
     1981 ccatgggctc ttggaaaatg ctggacgggg atttcagtca ccgtaaaccc gtacatttgc
     2041 gccaacgccg ggtaaaatcg gtgcatgttt ttaaatggag gaagtcgttt gaccacggcc
     2101 tgcctaaaga ctttcaagga acagccggta tctgaaatac catcatgcac cgcccaattg
     2161 cgaacctggt tggcaatttt tgacgaccac cggcggacca gactgtcatg tctggcctgt
     2221 cgtcgcccac acactaaatc atagcgttcc acaaagggga gaagtttcgc aaagtcattc
     2281 gggtcatact gcagatcccc gtccatggtc gcaacaagtt ctcccctggc ttcccgaaag
     2341 cctgcatcga atgccgccgt ttgcccatga ttccggtcta aatggatcac cagtaccgca
     2401 ggaaactgtc cggctaattg atccagcaaa ggtccactcc cgtccacact cccatcatcc
     2461 acaaacacga gttcaaacgc cgcggcccca ctttcaggcc tggattcaaa aaacttgagc
     2521 aattggctcg ccaagagcgg gatgttctcc cgctcatctt taatgggaat gacaacggaa
     2581 atccaatggc gcggcgggtg gagactcatg aatcggtcag actatgccac agatagcaaa
     2641 gttatgatgg gatacacgtt gcaaaccctc ctacccagca agcattttac tgaatatcaa
     2701 aaaaaccggt caaacggccg ttcttctcga gtgacccttc ccgagcttcg gtcatcttca
     2761 tgctaaactc ccaacctata actccagaaa gttgaccaag ggaacggcct tttgtaaggt
     2821 ggagaacatt attttggagg aacattcatg caagaagaaa ttctgcaagc ctatttggaa
     2881 atagagcaag cgatgcaacg ctattccatg ttattacagg accacgtgag ccaactcgga
     2941 actcagaccg atgaggaatc caaaaaccga ttccatagaa tgaaggccgg ctcaaaggcc
     3001 atgagagaca gtagccagat ttatctttct tatgcaaaat atgtagcgta tggaatgccc
     3061 gagggtgagg agctggctga agaggaggac ttacaagcct agcgagcctt cgtactgccc
     3121 aaacgactct ccgctctgat cataccgagg agacgaaaaa accccttctg ctggcaggaa
     3181 aacatggtcg gggcgagagg atttgaacct ccgacctctc gcaccccaag cgagtgcgct
     3241 accgggctgc gctacgcccc gacatgattc tcaccagtcg tattcccatc cttcattttg
     3301 tccgatgacc tacatcgtcg ctcgtcccag gatcttcaaa ataccttgca gttcaacctt
     3361 tgctttttcc acacggtctt ttgggacttg agcggttttt tctgaccgtt ttttgcgata
     3421 ccaatatcgc ttcaaacccg caatggtata cccttcatcg tagagcattc gtttaatttc
     3481 aaagaccgtt tcaatgtcgt ttttcgaata gactcgatgc tttccctgac ttttcttcgg
     3541 tttaaggaaa atgaattccg actcccaaaa ccgcaagaca tacgcaggaa gattcgtcaa
     3601 atcggcgact tcccctattt tataaaaggc cttatcctcg tgtttggttc caccccccat
     3661 acctgcactc accccacggt gtatctatca agagagtgaa cgagaccgat agcgaaattc
     3721 ttgccagtca agtattgaca tatttcttaa agagttggct tggccggaaa gtcaccactc
     3781 gtcttggcgt aatggcaatt tcctctccgg ttcttgggtt cctgcctttc cgctctccct
     3841 tgttccggac aacaaagttg ccgaatccgg caattttgac gacctctcct ttttgaagca
     3901 tggacttaat taaatcaaga acatgctcca ccatttccat tgagtcggtt ttttggatag
     3961 ctgccgtctc tgtaatgatt tcagcaatgt cagccttgcg cataaatcaa aaccccctca
     4021 caagtagtat cgaagaccgt tcatacggat tgtgaattca cgcttttgac cgttgttgca
     4081 ggcagcaacg taacgggcga cgcagatgat gtcaagaacc gggctcttca gaggattccc
     4141 ctttgtcagc ctatggtcgg agagacggtt ttatgtttgc ggcttctcct tcatgagttt
     4201 aaactcaata ctatcggcca acgcctgcca actggcttca atgatgttct ccgacacccc
     4261 gaccgtcccc catttctctt tgtgatcacc agactcaatg aggacccgca cacgagaacc
     4321 ggttccatgt cgtcctgtca acacgcggac cttatagtcc agcagcttca tttcctgtaa
     4381 ctggggataa aaatgctcca gagctttgcg aagggcatga tccagagcat tcactggtcc
     4441 atcaccaacc gccgcggtat gttcaaccac ttccccgact ttgaccatga tcgtggcttc
     4501 ggaaataggg tcttctttgg ttttccgttt ctcgacaatg atccggcacc ccaataattc
     4561 aaacgatggc gtataggtcc ccatggcttt ccgcactaac aattcaaaag acccttccgc
     4621 tccttcaaat tggtagcctt catattccaa cgtttttaga gaatcgagta attcgagcac
     4681 cttgggatta ttctgaggaa ggtccaggcc gaaagtttcc atttttccaa acacggcact
     4741 tcggccactg ctatcggaaa taagtacgcg gcgatggttg ccaacgacat ccgggttcac
     4801 atgttcatag gtaagcgggt ttttttgcac cgcatgaata tgcacccccc ccttatgcgc
     4861 aaatgcggta tctcccacat atggctgtcg tttgttggga gtcaaatttg ccatctcggc
     4921 cacataattg gaaatcgtgc gaagctgtgc taatcgacca tctcccagag cggttcgctc
     4981 cattttcaat tctaaattag ccatcacgga acacaggttg gcattcccgc accgttctcc
     5041 aataccattg atggttccct ggacctggat cgctcccgct tgcacggcag tcaatgaatt
     5101 ggcgacgccc atctccgcat cattgtgtgc gtgaatacct aggggaacag aacagttctt
     5161 gcgaaccttc gcaaagatct caccaatatc ccagggcat
//
LOCUS       DAORAQ010000174         5191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2299_length_5191_cov_65.623053, whole genome shotgun sequence.
ACCESSION   DAORAQ010000174 DAORAQ010000000
VERSION     DAORAQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2299_length_5191_cov_65.623053"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PKM72_11965"
     CDS             complement(<1..836)
                     /locus_tag="PKM72_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61549.1"
                     /translation="MHASSSWIVVGFLLFLSMSGMANAAQDFVVLGSEGVWVRQGSTI
                     FSGDVGANQTSIGPYLNGEQEMTIGHDVVVQNSNSGIIGDTVRLKSGSRIQNILVNTL
                     LGPGQILGTQATPVSFPLVSELPPVPPVHPGSKDVDVPAGGVLTLQAGRYGRLKARPG
                     AIVTLSGGLYHFQEWDIREDAQVLATKAVEIRVKGQIDTRRHTVVGPARGAATLTAAD
                     ILIIGIGINGTTGSIDDTPEAVKFGEGSKVRAKVYAPNGLLRFRAESTATGAFVGKWV
                     RMG"
     gene            complement(1618..>1921)
                     /locus_tag="PKM72_11970"
     CDS             complement(1618..>1921)
                     /locus_tag="PKM72_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61550.1"
                     /translation="FSHAGHYSPRVIVTNKQGQTFEATTEVVVENPEDLEAALNARWR
                     GMLAALAKKDVEGAVSYVATRKRDAMRQDWIVLADHLGEIAHLFSVPLQLIDGEGF"
     assembly_gap    1922..2021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2022..2246)
                     /locus_tag="PKM72_11975"
     CDS             complement(<2022..2246)
                     /locus_tag="PKM72_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61551.1"
                     /translation="MANATWNPEKRKLLFANLFGGESCVGFFHHELAQMSDRRFPLCR
                     EEIAELTFGSIAFFAYRLSKPLFPTQPRFLT"
     gene            complement(2370..2912)
                     /locus_tag="PKM72_11980"
     CDS             complement(2370..2912)
                     /locus_tag="PKM72_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61552.1"
                     /translation="MRQLIAFFCQLSVCICLVAGLVFSQTSSVSAQEITVTEDSFGCI
                     LDLPKVRNTRIKHDDPEKLKEAKRILRDSVPETEYPVGTILQLVPHEAMVKHSHDKFP
                     KTNGWEFFFLEVSKEGTKIQDRGDNVVNVSQGVTCLSCHQPAARFDFVCEKGHGCAPI
                     PFDDQKIAAIQKADPRCAKN"
     gene            complement(3321..>5191)
                     /gene="ftsH"
                     /locus_tag="PKM72_11985"
     CDS             complement(3321..>5191)
                     /gene="ftsH"
                     /locus_tag="PKM72_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNP61553.1"
                     /translation="AIQRHTGIEVRIANMEKKYRVSIWYFIIAFWGLIILQEMYFAAQ
                     HMDEVPYSQFKDWVQQDKVAEISITDKVIHGKLKSEKGGDSPQWFQTVRVDDPELVNM
                     LEEKHVEFAGVIVSTLWKDVASWVVPILVFAGIWFWILRKMGQGAGGGFMRIGKSKAK
                     VYIESDIKTRMSDVAGVDEAKVELMEVVEFLKTPEKFTKIGGRIPKGVLLVGPPGTGK
                     TMLAKAIAGEAGVPFFSISGSEFVEMFVGVGAARVRDLFEQATAKAPCIIFIDELDAL
                     GKARGTGPMMHEEREQTLNQLLVEMDGFDPRVGVIMLAATNRPEILDQALLRAGRFDR
                     QVLVDRPDRPGRAAILAIHAKNIKVAPDVDLDKIAAMTPGMVGADLANVVNEAALLAI
                     RRSRETAEHRDFEEAVERVIAGLEKRNRVLSVEERKRVAHHEVGHALVAMSLPGSDPV
                     QKISIIPRGVAALGYTLQLPIEDRYLFTRSELENRIAVLLGGRIAEELVFGEASTGAA
                     DDLQKATRIAKRMVKDFGMSDMLGTVALDDTVQPNFLKSMEGHSAPTYSEHTAQQVDQ
                     EVRRLIEEQGIRVRELLTRLRPVLLTGAETLLKAEIMMGEELMALLHAKEEEAIPS"
BASE COUNT         1307 a         1411 c         1117 g         1256 t
ORIGIN      
        1 cccattcgaa cccatttacc cacaaacgct ccggtagccg tgctttcggc tctgaaccgt
       61 aaaaggccgt taggagcata gaccttcgcc cgcaccttac tgccttcccc aaactttacc
      121 gcctccggtg tgtcatcgat ggaacctgta gttccattaa tcccaatccc aattataagg
      181 atatcagccg cagtcagagt tgctgcacct cgtgctggac caacaaccgt atgtctgcga
      241 gtatcgatct ggcccttcac gcgaatctcg acggccttag tcgctagaac ttgggcatct
      301 tcacggatat cccattcctg aaagtggtaa aggccgcctg aaagtgtgac aatggcacca
      361 ggtcgggctt tgagccgacc atagcgtcca gcctggagtg taaggactcc tccggccggc
      421 acatcgacat ccttcgatcc aggatgaacc ggtgggacgg gaggcaactc ggaaacaagt
      481 gggaagctga ctggtgttgc ctgggtccct aggatctggc ccggtcctaa gagtgtattg
      541 accaaaatat tctgtatccg agaaccactt ttcaatctga cggtatctcc aatgatcccg
      601 ctattactgt tttgaaccac gacgtcgtga ccgatggtca tttcttgctc accgttcaga
      661 taaggaccga tactcgtttg atttgcccca acatctccgc tgaaaattgt actaccctgt
      721 cgaacccaaa ccccctcact ccctagcacc acaaaatcct gtgcggcatt ggccattcca
      781 gacatactca gaaacaaaag gaacccgaca acaatccatg aggaactagc atgcattcga
      841 aaaatttttg tccaactcca tgtcattgaa gatattcccc tctttgctat acacccatga
      901 atattcaaaa tgtaatttcc ttaggagtga aagctccgga agtatgagag cgtaaggtaa
      961 tagcgcatat accccgttat aggggaagca atttttcaaa aaaaggtgaa cttaaaaaat
     1021 tattggatag gaaagatgag ctgaggaatc agctcttcct cccccaatag atagcggcaa
     1081 ggccaagccc cttaacttga tagttcgttc atgctgacca ctatcgggaa gactggggga
     1141 gtaccggtaa aaacatgtga gtgttgctta aattcgttga gaggatcacc aatagatagc
     1201 ctaaaggcta tccccaaaac agacggttcg aatcccaccg tacaatgaaa gtaaaaaaaa
     1261 taatcggatg aacaacacca ttacgaccag aaaacacgga ttggaacgcg gtacgaataa
     1321 tggccttgaa aaggcggcag gtatgatgaa tgaaaatgtt tactcgaggg gaaacactca
     1381 attatgaaag ctgtgaatga cgctgagtag cgttgttctc tcaaatttcc tagaaccgtg
     1441 aagttcccac aatcgccgga tttccagaat acgctcccac tcagagttgg gttttagaac
     1501 tttggtattt tagaaatttt tgatatacca ttgctgatcc gcttcccaca caaactctac
     1561 ctccaaggga ggttggattt tccccatggg caatgggtca actgatttgg ccaccactca
     1621 gaatccttcc ccatctatca gttgaagagg aacggaaaaa agatgcgcaa tctctcctaa
     1681 atgatcagcc agcacgatcc aatcctgtct catggcatca cgctttctgg tcgccacata
     1741 cgagacagcc ccttcaacat ccttcttcgc taaggccgcc agcatgcccc tccaccgggc
     1801 attgagagca gcttcaagat cttccggatt ctctaccacc acctcagtcg tcgcttcaaa
     1861 ggtttgcccc tgtttattgg tgacaatcac ccgaggagaa taatggcccg catgtgagaa
     1921 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgtgaggaa acgcggctgg
     2041 gtggggaaaa gcggcttgga tagcctgtag gcaaagaatg cgatggaacc aaacgtcagt
     2101 tcagctatct cttccctgca caacgggaat cttcgatcgc tcatttgagc aagctcatga
     2161 tggaaaaaac caacacagga ctcaccacca aacaggtttg cgaaaagcaa cttcctcttc
     2221 tccggattcc acgttgcgtt tgccaaacat ggcggtgccg atttctaccg cccgaatgcc
     2281 agccaaaaat taagtcgatg agtctgtgag acttcttatt gggctcgaac cttccggcaa
     2341 gctttatttc acctcaaaac tatccccacc taatttttgg cacagcgggg atctgctttt
     2401 tgaatggccg cgatcttctg atcatcgaac gggatagggg cacagccatg ccccttttca
     2461 cagacgaagt caaacctggc cgctggctga tgacaactta agcacgtgac cccctgcgaa
     2521 acattgacga cgttgtctcc ccgatcttgt atcttggtgc cttctttgga gacctccaaa
     2581 aagaagaatt cccagccgtt cgtttttggg aacttgtcat gcgaatgttt caccatcgct
     2641 tcatggggaa caagttgcag gatggttcca acgggatatt ccgtctccgg gacgctgtcc
     2701 cgtaagatac gcttggcctc tttcaatttt tccggatcgt catgcttgat ccgcgtattt
     2761 ctgactttcg gcaaatcgag aatgcaaccg aacgagtcct cggtcaccgt gatttcctga
     2821 gctgacacgc tggaagtctg actgaacaca agccccgcta caagacatat acaaacagag
     2881 agttggcaga agaacgcaat aagttgacgc attgacctac tcctttcatt ccgccctaac
     2941 gagcatcccg cgaacgtgaa tcttaagctg tccccactgt atacccattc atatttcatc
     3001 tattcgggga gcaattttcg aagcatcata cctcaaggca gaagaattaa ggaacaactg
     3061 actatcgtgc ttcgaaccgc acttcggcaa tgctcgtttt tttgattatt ccactattcc
     3121 gtatcgaacg gagaaatgcc tgagaaaagg cgctttctca agaacgcaaa acggttttga
     3181 gttttttttc gtcgaacggt ataccaggga aaaaagcttg atatcgccgg gaatgaccga
     3241 aaaggcaggt gattgcagag gggataatcc ggacagcatt caggccaatg gcaggtctgc
     3301 ttccggattg tcgggtggga ttaactggga atggcttcct cttccttggc gtggagaaga
     3361 gccatgagct cttcacccat catgatttcc gccttcaaca gggtttcggc cccggtcaac
     3421 aacacaggtc gaagcctcgt caacagttcc cggacgcgaa tcccttgttc ttcaatgagg
     3481 cgtcgcactt cctgatcgac ctgttgagcg gtgtgctcgg aataggttgg ggcggaatgg
     3541 ccctccatac ttttcaagaa gttcggctga acggtatcgt ctaaagccac cgttcccaac
     3601 atgtcactca tgccaaaatc tttgaccatt cgcttagcga tcctggtcgc cttctgcaaa
     3661 tcatctgccg caccggttga ggcttctcca aagaccagtt cctccgcaat acgtccacct
     3721 aataagacgg caatgcgatt ttctaattca gaacgggtga ataaatacct gtcttcgatc
     3781 gggagctgta acgtatagcc caaggctgca acgccgcgcg gaatgatcga gattttttgg
     3841 acagggtctg acccgggcaa cgacatcgcc accaacgcat gcccgacctc atgatgggcc
     3901 actcgtttgc gttcttccac actgaggact cggtttctct tttccagtcc ggcaatcacc
     3961 cgttccacgg cttcctcaaa atcccggtgt tccgccgtct cccggcttcg tcggatggcc
     4021 agtagggccg cctcattcac cacgttggct aagtcggcac caaccattcc cggtgtcatt
     4081 gcggcaattt tgtctaaatc gacatccgga gccactttaa tgtttttggc atggatggcc
     4141 aaaatagcgg ctcttcccgg ccgatcaggg cgatcgacca gcacctgtcg atcaaaacgg
     4201 cctgcacgca acagcgcctg atccaaaatt tcaggccggt tggttgcggc caacataatg
     4261 acccccactc gaggatcaaa cccatccatt tcaaccaata attgattcag cgtttgttca
     4321 cgttcttcat gcatcatggg ccccgttccc cgggcttttc ccaatgcatc caactcatct
     4381 atgaaaataa tacatggcgc cttggctgtg gcttgttcga acaaatcgcg cacgcgggcc
     4441 gctccgactc ccacaaacat ttccacaaac tccgacccgc taattgaaaa aaacggcacg
     4501 ccggcttctc ccgcaatcgc cttcgccagc atagtttttc cggtgccagg gggcccgacc
     4561 aggagcacac ccttggggat ccgtcccccg atcttagtga atttttccgg ggtttttaaa
     4621 aattcaacca cttccatgag ttccacttta gcttcatcga ccccggccac atcactcatg
     4681 cgagtcttga tatcgctttc aatatagact ttagctttgg atttcccgat tcgcataaac
     4741 ccgccccccg cgccttgccc catcttccgc aaaatccaaa accaaatccc ggcaaagacc
     4801 aggataggga cgacccagga agccacgtcc ttccagagcg tactaacgat gacgccggca
     4861 aattccacat gtttttcttc caacatgttc actaattccg gatcgtccac ccggaccgtc
     4921 tgaaaccatt gagggctatc ccctcctttt tccgatttca attttccgtg aataacctta
     4981 tccgtaatgg agatctcagc caccttatct tgctgaaccc aatctttaaa ttgactgtag
     5041 ggcacctcat ccatatgttg ggcagcaaaa tacatttcct ggagaataat gagtccccag
     5101 aacgcaataa tgaaatacca aatagagacc cgatattttt tttccatatt tgctatccta
     5161 acctctattc ctgtgtgacg ctgaatcgct a
//
LOCUS       DAORAQ010000175         5174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2306_length_5174_cov_61.561047, whole genome shotgun sequence.
ACCESSION   DAORAQ010000175 DAORAQ010000000
VERSION     DAORAQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2306_length_5174_cov_61.561047"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKM72_11990"
     CDS             complement(<1..801)
                     /locus_tag="PKM72_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP61554.1"
                     /translation="MLLEYPKGIFLIATLIALIVANHLYFKLALHAGIVDVPNQRSSH
                     LLPTIRGGGVVFVVALLFWFSLNAKLYPWFIAAVLLIAIVSFADDVRSLSASVRFFVH
                     LISIILLGIQVELFAQPLWIFFPVIIVGIGVINSFNFMDGINGITGIYGLVNLLSFFY
                     INHEIVHFIDDSLPLYILMSILVFLFYNFRRRAVCFAGDVGSITLAFIQVFLLVKLII
                     ETQQFSWIFLFLVFGVDSVVTILYRLRKGENIFKPHRSHLYQYLSNEMG"
     gene            complement(782..1888)
                     /locus_tag="PKM72_11995"
     CDS             complement(782..1888)
                     /locus_tag="PKM72_11995"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNP61555.1"
                     /translation="MVFAILDAIAHKVNTNNAADLSICPLSKYSLRFIAAFKMSTNLS
                     VPDPEFRLGNGWGNTLFSSRMTRILLTGASGFLGKQLVEKLKADFELLTIGRSKGNDI
                     AFDFSSGIYRGLGSLDCIIHAAGLAHKIPSSQSEQDEFFRVNLGITDNLLKSLLHERT
                     LPKSFLFISSVSVYGLMRGENISEDHPLQANDPYGKSKVEAEYLLRQWCEVHGVALTI
                     LRLPLLAGAFPPGNLGAMINAMKNGRYFSIDGGKARKSIVMASDVAKIIPTAIDFPGV
                     YHLTDGHHPSFFDLEGVISGQLGVSRPRNIPAFAARCIGFCGKLVDIITPGRSPITPR
                     KVEKIISTLTFNDQKARQVLGWNPSRVIDAFRIS"
     gene            2327..3520
                     /locus_tag="PKM72_12000"
     CDS             2327..3520
                     /locus_tag="PKM72_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP61556.1"
                     /translation="MNIVYFYQYFSTSEGTWGTRVYEFTKRWAEQGHHVTVVTSIYSK
                     SDLHSNTRISDQLIDGIHVKVINVLIDNQQSVLKRIWTFAQYAMISTWYALTLRADVV
                     VASSGPITVGIPGLVARMRGRKLVFEARDLWPEGAVKMGLLKNSIVIKLLYALEKLCY
                     KSSHTIIGLSPGIQQDIQARFPSCRVESVTNAANIELFSNTFPPPAGLQAGSYAIYFG
                     NIGEVNHPGYLLDAASILLQQGRSDIQVVFIGEGQLKKALIERVSKERLINVLSLNLM
                     PKSTLVRYVQHALASVVPLKPLPVFDTSSPNKLFESMAAGVPVIQTTQGWIKDFLNDH
                     EIGFTVNAHNPKELADKLIFMKDNPHLSQEMGKRARQVAKKYFDKDYLADRMLDILKK
                     VAHEN"
     gene            complement(3979..5070)
                     /gene="wecB"
                     /locus_tag="PKM72_12005"
     CDS             complement(3979..5070)
                     /gene="wecB"
                     /locus_tag="PKM72_12005"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNP61557.1"
                     /translation="MSKICTVVGARPNFMKMAPVILELKRRELPQFCVHTGQHYDSQM
                     STIFFDELGMPKPDAFLGVGSCSHAEQTARIMTSFEKLCLEQKFTLVIVAGDVNSTLA
                     CALVAAKLHIPVAHVESGLRSFDRSMPEEINRIVTDHLSSILFTTEPSGNDNLHNEGV
                     FVGQTHFVGNTMIDSLKAHLDKALAGKPWQKLDIEPHSYGLVTLHRPANVDDLPTLSQ
                     IVLALKEISGELPLLFPVHPRTRAGLEQILSDWAPIRIIEPLGYLDFLALMAKAKLVL
                     TDSGGIQEETTILKVPCVTIRPNTERPITVEAGTNRLAGVTKEGIVAAAKAALSQKSL
                     NENPPALWDGQAASRIVDVIEKFCAKTHK"
BASE COUNT         1615 a         1150 c         1072 g         1337 t
ORIGIN      
        1 ccccatttca ttcgaaagat attggtataa atgtgaccgg tgtggtttaa atatattttc
       61 accctttctc aaccgataca aaatagtcac taccgagtca acaccaaaca ccaaaaacaa
      121 aaaaatccaa gagaattgtt gcgtttcaat gatcaacttc actaacaaaa aaacctgaat
      181 gaatgccagg gttatacttc caacatcacc ggcaaagcac acagcacgtc ttcggaaatt
      241 ataaaaaaga aataccaaaa tactcatcaa gatatacagt ggaagactgt catctatgaa
      301 atgaacaatt tcatgattaa tatagaaaaa ggataagaga ttaaccagac catatattcc
      361 cgtaatacca ttgatgccgt ccataaaatt aaaggaattt atgactccaa taccaacgat
      421 aataaccgga aaaaaaatcc atagcggctg ggcaaacagc tcaacctgaa ttccaagcaa
      481 aataatgctg atcaggtgca caaaaaaacg gacggatgca gacaagctcc gaacatcatc
      541 agcaaaacta acgatagcaa ttagcaacac tgcagcaata aaccatggat agagcttggc
      601 attaagagaa aaccaaaaca ggagagctac gacaaaaaca actcccccgc cccgtatggt
      661 tggcaaaagg tgtgaactgc gttgatttgg tacatcaaca attcctgcat gaagggctaa
      721 tttaaaataa aggtggttcg caacaataag tgcaattagt gttgcgatta gaaaaatgcc
      781 tttaggatat tctaaaagca tcaatcactc gactgggatt ccaacctagc acttgccggg
      841 ccttttgatc attgaatgta agcgttgaaa ttattttttc aactttcctg ggggtaattg
      901 gcgatctccc aggagtaatg atatccacta actttccgca aaaaccaata caacgggcgg
      961 caaaggctgg aatatttctc ggcctactaa caccgagctg cccagatatg acaccctcaa
     1021 gatcaaaaaa tgacggatgg tgaccatccg tcaaatgata aacaccagga aaatctattg
     1081 ctgttgggat aatcttagct acatcggaag ccatcactat gctctttcta gcttttcccc
     1141 catcaatact gaaatagcga ccatttttca tggcatttat catggcaccc aggtttcctg
     1201 gtggaaatgc gcccgccaaa agcggcaacc gtaaaattgt aagtgcaaca ccatgaacct
     1261 cacaccattg ccgcaataaa tattctgcct caactttact ttttccatag ggatcattcg
     1321 cctgcaatgg atggtcctcg gagatatttt cacccctcat caatccataa actgaaaccg
     1381 aacttataaa caaaaatgac ttcggcaatg tcctttcatg aagaaggctt ttcagtaaat
     1441 tatcagttat acctagattt accctaaaaa attcatcttg ttccgattgt gatgaaggaa
     1501 ttttgtgggc aagaccggca gcgtgaataa tacaatccaa tgaccccaaa cccctgtata
     1561 ttccagaact aaaatcaaac gctatatcat ttcctttcga tcttccaatc gtaagcaatt
     1621 caaaatctgc cttcagcttt tcaaccaatt gcttgccaag aaaccccgaa gcacctgtga
     1681 gtaagattcg ggtcattctg ctagaaaata aagtatttcc ccatccatta ccaagtctga
     1741 actctggatc gggtacggat aagttggtcg acattttgaa tgcggcaatg aatcttagtg
     1801 aatatttgct taaggggcat atagagaggt ccgccgcatt gttggtattg accttatggg
     1861 cgatcgcatc aagtatggca aagaccattc caggtttcaa tgccgccgtc ccctgcgggg
     1921 gtgaattaaa atctacccag gacgccagta tcgtctcatc gcgatagtta ttacatttct
     1981 tccttcctgt ctgcccagat ctctggcaag aagcaaggcc gcggaaagtt cagaaaatgc
     2041 gggagtggct tcggaatgaa gtcatgaatg agtggcgccc acagttggag agtgccacat
     2101 tgccgaagaa agtgcgatcc atttatcggg tggccgggac ttggtcagga aacgaggaca
     2161 cctctgtcat gatggttttc atcctccgat caggctcgaa actcatcccg cttcagactc
     2221 acttcctgtt gaaaactcgc cagacgctca gactcatttt ctatgggaag agacttaaaa
     2281 tttacatagg caaaagttac gtttagaata cacatttgca ctgcctatga atatcgtcta
     2341 tttctaccaa tatttctcta catcagaagg cacgtggggt acgcgtgtct atgaatttac
     2401 caaacgctgg gcagagcaag gacatcacgt aacggttgtc acctccattt actccaagtc
     2461 tgatcttcat tcaaatacac ggatttctga tcagctgatt gacggaatcc acgtgaaagt
     2521 aatcaacgtg ctgattgata accaacagtc tgttctaaag aggatatgga catttgctca
     2581 atatgccatg atttcgacat ggtatgcact taccctaaga gccgatgtag tggttgcttc
     2641 ttcgggcccc attacggtgg gtatccccgg actagtagca cgaatgcggg ggagaaaact
     2701 ggtctttgaa gcacgtgact tatggcctga gggggctgtg aaaatgggcc tactgaaaaa
     2761 ctccatcgta attaaactct tatatgcttt agaaaaactc tgttacaaaa gttcacacac
     2821 aatcatcgga ctttcccctg ggatacagca ggacatccag gcaagatttc cgtcgtgccg
     2881 ggtggaaagc gttacaaatg ctgccaatat tgagttgttt tcaaatacct ttcctccccc
     2941 tgcgggcctt caagcaggca gctatgcgat atattttggc aacatcggtg aagtgaatca
     3001 tcctggatac ttgctggatg cagcctcaat tttattgcag cagggcagga gtgacatcca
     3061 ggtggtgttt attggagaag gacagttgaa aaaagcattg atagagcgtg ttagcaaaga
     3121 gcgattaatc aatgttttat cactgaatct tatgccgaaa tccactctgg tgcgttatgt
     3181 tcagcatgca ttggcttcag ttgttccttt aaaaccctta ccggtattcg acacctcctc
     3241 acccaataaa ttatttgaat cgatggccgc cggcgtgcca gtaattcaga cgacacaggg
     3301 ttggattaaa gatttcctta acgaccatga aatagggttt acggtaaatg cccataaccc
     3361 taaagagctt gcggacaaat tgattttcat gaaagataat ccgcatctct cgcaggaaat
     3421 gggtaaacgg gccagacagg tagctaagaa atattttgac aaggattatc ttgcggatag
     3481 gatgcttgac atcctaaaaa aagtggcaca tgaaaactga gccacgtttt tcagaaacac
     3541 ccctctatgg acatggcctg aaacttggaa gtaggtggac caaattatag tacttcctaa
     3601 actcaggtat gcgtggattg aggtaaaaaa gagagaagat taatgggcct gatattgggt
     3661 agggtcattt ttttcgcgga caatatttca aaaagaagct ggaggtataa tggtgtccat
     3721 ttgcaaatga agggaataca tctcagaact tcgaacccga aatgactgtc aaaaaggtag
     3781 ccatctcaat ttttaaatta tctcctgcca caaattacac taccattcga tttctgaaca
     3841 gaaatttaaa ggaattggga acccatccaa tctcctgaag aaccagctcc taatcaagag
     3901 tacctgaaac attcatcaga aaaatttatc aacggatgca agggtattac accctaaaaa
     3961 acattcaata aaaccagctt acttgtgtgt tttcgcacaa aatttttcaa tgacatcaac
     4021 tatcctgctg gccgcctgcc catcccaaag agctggtggg ttttcattta aagatttctg
     4081 tgaaagagcg gctttggcgg ctgcaacaat gccttccttg gtgactcctg caagtctatt
     4141 ggttccggct tctactgtga tgggacgctc agtgttgggg cgtatggtta cacaaggaac
     4201 ttttaaaata gtcgtttctt cttgaatccc gcctgaatcg gtaagaacca gtttagcttt
     4261 ggccattaac gccaaaaagt ctaaatatcc taacggttcg atgattctaa taggagccca
     4321 atcagataat atttgctcca gccctgcacg agtacgaggg tgcacgggaa ataaaagtgg
     4381 aagttcgccg gaaatttctt tcagagccaa aacaatctgg gaaagagtgg gaaggtcgtc
     4441 aacattagca ggacgatgca gggtcaccaa accataggaa tgaggctcaa tatccaattt
     4501 ctgccaaggt ttcccagcca aagccttatc caagtgagcc ttcaaactat caatcatcgt
     4561 attgccaaca aaatgagtct gacctacaaa aactccttcg ttgtgcaaat tgtcatttcc
     4621 actgggctcg gtggtaaaaa gtatggagga aagatgatcg gtcacaatcc gattaatttc
     4681 ctctggcatt gaacggtcaa aggaacgcaa tccagattcc acatgggcaa ccggaatatg
     4741 aagtttggcg gcaacgagtg cacaggcgag tgtcgaattg acatcacccg cgacaataac
     4801 caaagtaaac ttttgctcca ggcagagttt ctcaaaagag gtcattatac gtgcagtctg
     4861 ttccgcatgc gaacacgatc caacgcccaa aaaagcatca ggcttgggca tacctaattc
     4921 atcaaaaaaa attgtggaca tttgcgaatc ataatgttgc cccgtatgca cacaaaactg
     4981 cggcaactcg cgccgtttaa gttcaagaat aacaggcgcc attttcataa aattggggcg
     5041 tgccccaaca accgtacaaa ttttactcat agactggttt ccaggtagtt aatgaatctg
     5101 gcatcaatag cagtagctaa aattttgttc agccacggga catctaattt cgccataatt
     5161 tttgtggaga tggg
//
LOCUS       DAORAQ010000176         5089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2359_length_5089_cov_71.254072, whole genome shotgun sequence.
ACCESSION   DAORAQ010000176 DAORAQ010000000
VERSION     DAORAQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2359_length_5089_cov_71.254072"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1039
                     /locus_tag="PKM72_12010"
     CDS             230..1039
                     /locus_tag="PKM72_12010"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61558.1"
                     /translation="MGYKIKDLSNKSPMVEQAQFLSSKERFLFFVEENRALVWGGIFL
                     VLAVIVAIVTMGWLNQTNQEHAWELEGQAQTIYLDRPLDDVKKGQENIQKASSLFKDI
                     LEQFPGTPSAGVSSFLLGNSMMEEKNYQGAIEVYTSWIKEYGQDHIFVGLVQQRLGFA
                     YLLNGDRESALKAFDAVLADPHSLNKDQVVFELAKIAEADENTAEAVGQYKKVIQEFP
                     LSPFASEAALRVKVLAPEEAKDSPSSETGELDGIEKSSPEAPQVQDKGEGK"
     gene            complement(1071..3029)
                     /locus_tag="PKM72_12015"
     CDS             complement(1071..3029)
                     /locus_tag="PKM72_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNP61559.1"
                     /translation="MNHLDKTLQVQRSSVSLRPAAIASNLLFGSSVILITLALCPQSS
                     LGEPSFTPSNDSTEEMTFNYAIAHSHPHQSTSIETQTSPNSTIQNNQQSSILIDQTEK
                     PTPGMTGLSSEDPSNPSSVDDLAQTPSQQIQSSDTGKYITPSSVDVSQEPNTSEDQRI
                     FLSGENTLEALNAQTGFSINSSTKLRFYPADSSEQRSQIVLARFAKAFDQAWESTSLR
                     KPGAYGTIPLVLNDSVEKNLEYFQYGIHDRFQSYLDRFHHYRDLVEPVFHELGLPPEL
                     MYLSLVESGFNPRAFSRSRASGPWQFMKATGRVYGLDVDWYLDERRDPIKSTVAAAHH
                     LRDLYDQFGSWPLALGAYNAGSGKISRAIKKTGTRDFWKIRQTRHIRRETKDYVPRFI
                     AATLIAQNPTAYGFSTPDGDRHEFEEVLITKRVHLSAVTQQTGIPVEELRRLNPELRR
                     SIVPSLTGPGYYLKVPLGMASLVEELHPTLTVWSQPPPPPTEWHTVRRGESLSVVAKR
                     YRMKVSQLKDMNNLRSNIIRVGTKLRVRGEVDDDYADSETTWYRVRSGDSLGSIATRF
                     RQSVNALMRMNNISSHIIHPGDRLRVKGNPSVSPSNKNDSKWYKVRRGDSLWTIAQQF
                     RMSVKDLRALNNLSSSIIQAGHMLMVSQ"
     gene            complement(3740..4084)
                     /locus_tag="PKM72_12020"
     CDS             complement(3740..4084)
                     /locus_tag="PKM72_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNP61560.1"
                     /translation="MSLADNTCVPCRGGVPPLEPGKIQDLLGQLETGWTLNAQGHIEK
                     TYTFDNFADALAFTNRVGNVAELEGHHPDIYLAWGKCKVEIWTHKIQGLTESDFYLAA
                     KADRAFHLPAAH"
     gene            complement(4451..4717)
                     /locus_tag="PKM72_12025"
     CDS             complement(4451..4717)
                     /locus_tag="PKM72_12025"
                     /inference="COORDINATES: protein motif:HMM:NF021169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HNP61561.1"
                     /translation="MNQSSNTVKVFDTWVQGKTGTIHFDVMTTDEDTALRLAKTYLAS
                     LGEAEAVITTEECRFCHNEPLAFVSQTQQQEFTQNGGFILPMPS"
     gene            complement(4714..>5089)
                     /locus_tag="PKM72_12030"
     CDS             complement(4714..>5089)
                     /locus_tag="PKM72_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61562.1"
                     /translation="IPHSFAQPCSSWILICLFPTGCLVVPPPQSYENTLIHYEPLVGM
                     NIRNLQWQTLAVNATQPWQKSNVHLTPGTNLKILAMGKWGLAPGSRNFHQTLECHDCI
                     YSRHFDQELAEPDDGNRKKGHS"
BASE COUNT         1357 a         1036 c         1317 g         1379 t
ORIGIN      
        1 cgacatctgc cgtgtaaagg cagcgctctg ccagctgagc tatccgccta aaaattaaaa
       61 cgctacaatt cgtccattat aacagggagt taggccttgt caactcaaat gaaatagtgt
      121 ggaatactgt ttgacatgaa caggacacgt gggtataagg cagcacaccc aaattctgaa
      181 tgcgagaaaa aatatttggt cgtcctagtg acgagaggga aaagtaatta tgggatataa
      241 aattaaagac ctttctaata aatctcctat ggtggagcaa gctcagtttc tatcgagtaa
      301 agaacggttt ttgttttttg tggaggagaa tcgggctctg gtatggggag gaatttttct
      361 tgttctggct gtgatcgtgg cgattgtgac gatgggttgg ctcaatcaaa ctaatcaaga
      421 gcatgcatgg gaactagaag gtcaggcgca aacgatctat ttggatcgtc cactagatga
      481 tgtcaaaaag ggacaagaga atattcaaaa agcttcaagc ctgttcaagg acatccttga
      541 gcaatttcct gggacgccca gcgccggagt ctcctcattt ttattaggca acagtatgat
      601 ggaggagaaa aattatcaag gggcgataga ggtgtatact tcgtggatca aagagtatgg
      661 gcaagatcat attttcgtcg gattagtcca gcagcgattg gggtttgcct atttacttaa
      721 tggcgatcga gagtccgccc taaaggcatt tgatgccgtg ctggccgatc ctcattcgtt
      781 aaataaagac caggtagttt ttgagctggc gaagattgca gaggctgatg aaaatactgc
      841 tgaagcggtt gggcagtata aaaaggttat ccaggaattt cctttatctc cgtttgcgtc
      901 ggaagctgcc ctccgggtga aagtgttggc accggaagaa gctaaagatt ctccttcctc
      961 ggaaacgggt gaactggacg ggattgagaa gagcagtcct gaagccccac aggttcagga
     1021 taaaggagag gggaaatagt attagttaag tggctcaccc aataggtggg ttattggctc
     1081 accatcaaca tgtggccagc ctgaataatg ctggaagaga gattattgag cgctcgcaaa
     1141 tctttcacgc tcattctgaa ttgttgggca atcgtccata aactatctcc acgcctgacc
     1201 ttataccatt tagagtcgtt tttgttcgac gggctgaccg aaggatttcc ttttacccgc
     1261 aaccggtccc ctggatgaat gatgtggctg gagatattgt tcatccgcat cagtgcattc
     1321 accgattgtc gaaaccgagt cgcaatgctt ccaagtgaat ccccggatct gacgcgatac
     1381 caggtggttt cagaatcagc gtaatcgtca tcaacttctc cacgaactcg gagtttggtg
     1441 cctactcgta tgatattgct ccgcaaatta ttcatgtctt tcagttgact aactttcatt
     1501 ctatatcgtt tagctactac cgataaactc tctcctcttc tgactgtatg ccattccgtt
     1561 gggggaggag gaggttggct ccagacggtg agtgtcggat gcagttcttc gaccaatgaa
     1621 gccatgccga ggggtacttt caggtagtag cctggacccg taagactggg cacgatgctt
     1681 cgtcgaagtt ccgggttgag tcgtcggagt tcctcaaccg gaattcctgt ttgttgtgta
     1741 acggctgaaa gatgcacgcg tttcgtaatc aggacttcct caaattcatg gcgatcgccg
     1801 tcaggagtac taaatccata ggccgttgga ttttgtgcga taagggtcgc cgcaataaag
     1861 cgaggcacat agtctttggt ttcccgtctt atgtgccgtg tctgtcgaat tttccaaaaa
     1921 tcacgggttc ctgttttttt gatggcccgt gaaatctttc cacttcctgc attgtacgct
     1981 ccgagggcga gaggccagga gccaaactgg tcatataaat cacgaagatg atgagcggcg
     2041 gcaactgtgg atttaatagg atcccgccgt tcatccaaat accagtcgac gtcaaggcca
     2101 tacacacgac cggtcgcttt cataaattgc caggggcctg aggcacggga tcgagaaaat
     2161 gcacgagggt tgaacccact ttcaacgagt gaaagataca tgagttccgg tgggaggcct
     2221 agttcatgaa aaacgggttc cacaaggtct cgataatgat gaaagcgatc gagataggat
     2281 tgaaatcgat catgaatccc atactgaaaa tattcaagat ttttctctac cgaatcgttc
     2341 agtacaaggg gaatggttcc atatgcccct ggttttctca gcgaggtgga ttcccatgct
     2401 tggtcaaacg ccttggcgaa tcgggctagg acaatctgcg accgttgctc agatgagtca
     2461 gcagggtaaa aacgtaattt ggtagaggag ttgattgaga acccagtttg agcgtttagg
     2521 gcttcaagtg tgttttcacc agacaaaaag atccgctggt cttctgaagt gttaggttcc
     2581 tgggaaacat caacggatga gggagttatg tattttccag tatccgatga ttggatttgc
     2641 tgtgagggtg tttgagccag atcatctaca gaagacgggt tcgaggggtc ttcggaagat
     2701 aaccctgtca tacctggagt tggtttctca gtttgatcta tcagaataga tgattgctga
     2761 ttgttttgaa tggtgctatt cggagacgtt tgggtctcaa ttgaggtgct ttggtgaggg
     2821 tggctatgtg caatagcata attaaaggtc atttcctctg tggagtcatt tgaaggggtg
     2881 aacgagggtt cgccaagaga ggattgtggg caaagagcca acgtgatcaa aatgacactt
     2941 gagccaaaga ggagattgct ggcaatggca gccggtcgca gagaaacgga ggaacgttga
     3001 acctgtaagg ttttatctaa gtgattcatt ttataaggca tttcggcaaa tctgtgcaca
     3061 acttaatagg ctaccctcgc aagaaaagtt tgtcaaacat atttatgtat ttttgatctt
     3121 ttccataggt agaactaaat tgcacaaacc aaaccaaaag ttaagtgtat gaaataaaga
     3181 aaaatatgat gggagggagg atgagagtgg tgtttttagt ccagttatta ggcaaaaatg
     3241 tcaccgatga ggattccaag gtatgaggat atcggcgaga gatagaatgg cgtcttctca
     3301 gatcctggaa aggagaaacc ctgcaaggct ttgcgaatag gttcaacgga ttggggctgc
     3361 tcattgcaaa attctaggat gaaggcctga ggtccttcaa atcgttcact ggtcggagta
     3421 agcctcttaa atattccagg agggagcaat atctcgatat cgcctacatt ttgtattacg
     3481 aaaccgagat ttatgggtga aatggaaatt gattggatag gaatgactgg atgctaagcc
     3541 cttgtactct tttagccaag cccagggggt cttagttgga tcgaaggaaa tttgattaac
     3601 taaatgtcaa tggcttcatg tgcgaccttt gttctaagag attggaaacc ggtggtgacc
     3661 tgaggcgaag gtggctgaga ttgttttgcc tctaaagaga ccgagtgaat caaattctgg
     3721 ctggttccta gaagagcctt cagtgggcag caggaaggtg aaaggcacga tccgccttgg
     3781 cagctaaata aaagtcactt tccgtgaggc cttgaatttt gtgggtccaa atttctactt
     3841 tgcatttgcc ccaggccaaa tagatatcag gatgatggcc ctcaagttcg gcaacattgc
     3901 ccactcgatt agtaaaagcc aaggcatctg caaaattgtc aaaggtatat gttttttcaa
     3961 tatgaccttg agcatttagg gtccatccag tctccaattg acctaacagg tcctggattt
     4021 tcccaggttc caacggcggt acgcccccac gacatggcac gcatgtatta tctgctaggc
     4081 tcattttctt cctccttacg tcctgaagat ctgcggtgga ttgtcttttt cactctattt
     4141 tttaatctta tacccgagaa aacgtgattt tccccatatc cttcgtgtaa atcagtttcc
     4201 attgagaagt gaaccccatg caatcgtgat gtcacattaa agccctgctt actctactga
     4261 gtatagccat gaccaagtgc ggtgcacaat tccaagggcg cctggctatc ggaatgttct
     4321 gcgcagtcca gcctagcccg gcatcgagcc acactcatat acgggtcaaa tatgactcta
     4381 ccaaatccca aacctatgat ttttgttacc gacccttttg acaaaaaaac agaatgaggg
     4441 caggggaggg tcaggaaggc ataggaagga taaaacctcc attttgggta aactcctgtt
     4501 gctgggtttg agagacaaag gcgaggggtt cattatggca aaatcggcat tcttctgttg
     4561 tgatgacggc ctccgcttca cccaggctgg caagataggt tttggctaat cgaagagcgg
     4621 tatcttcatc tgttgtcatg acgtcaaagt gaatggtgcc agtttttcct tgcacccagg
     4681 tatcaaaaac tttgacggta tttgaggatt ggttcatgaa tgtcctttct tccggttgcc
     4741 atcatcgggt tcggctaact cctgatcaaa atgacgactg taaatacagt catggcattc
     4801 aagtgtctgg tgaaaattcc tggatccagg tgccaggccc cactttccca tggcgagaat
     4861 tttcagattg gttcctgggg tgaggtgcac attggatttt tgccagggtt gggtggcgtt
     4921 caccgcaaga gtctgccatt ggagattgcg gatattcata ccgactaagg gttcatagtg
     4981 aataagggta ttctcgtagg attgaggagg aggaaccacc aggcagccgg tgggaaatag
     5041 acagatcaga atccaagagg aacacggttg cgcgaatgaa tgaggaata
//
LOCUS       DAORAQ010000177         5076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2367_length_5076_cov_65.388170, whole genome shotgun sequence.
ACCESSION   DAORAQ010000177 DAORAQ010000000
VERSION     DAORAQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_5076_cov_65.388170"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKM72_12035"
     CDS             <1..287
                     /locus_tag="PKM72_12035"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP61563.1"
                     /translation="FEWSDWRNGNFWWLQSVYVHPDQRQQHIFRQLYEYVEGQAHLNR
                     EPICGYRLYVEEKNIQAHQTYAKLGFQETAYHMYEKEFSWTSPSFPQSIV"
     gene            342..1628
                     /locus_tag="PKM72_12040"
     CDS             342..1628
                     /locus_tag="PKM72_12040"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNP61564.1"
                     /translation="MRLFRWRQGVKTCLFCIVGLSWAWFPPAVLANPSAALMELEPRP
                     HVRDLGITIGRYSPGEMNAITDVQGVKVGHVTIYEGNGALQVGQGPVRTGVTVIIPRE
                     DVWHHKVPAGAFVLNGTGEMTGLAWVTESGFLEYPIALTNTLNVPRVADGVMSWMMQR
                     YPGIGISDDTLTPVVAECDDSRLNDSQGRHVSSEDVVRALETASIGPVPEGSVGAGTG
                     MVSYQFKGGIGTASRILPSEEGGYRVGVLVNANHGRRHEFMISGVPVGQVYDTDLATV
                     SQQNSQESTMATPGEIPADFPQKRQDSGSIIIIIATDAPLDARQLSRLSRRATIGLAR
                     TGSISHHGSGDFVLAFSTGNVIPHYPEERTFFMAHLADTHINPLFQATVEATEEAILN
                     ALLQATTVTGRDGRRFEAISIDRLRSILNVYRPPTR"
     gene            1720..3000
                     /locus_tag="PKM72_12045"
     CDS             1720..3000
                     /locus_tag="PKM72_12045"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNP61565.1"
                     /translation="MAGIARLKSDLITMQCHVALSQESHPEVAAHAVTASVQEALGEA
                     GCHLAFVFFSPHFSSDIQRMVDIIHETLRPQTLVGCMGQGIIGADQEIEEHPGLVLWG
                     LRSTDMRVVPFMLTPKIEGEVASLEGWPVGLESTPHPYTFFLFADPFSTPIDELLSLL
                     EEQAPGSSAIGGIASGGMDVGESRLVFNRDIIGSGVVGVAVQGGVEIRTVVSQGCQPI
                     GERYVVTKVDQNVIQELGGIPPLERLESTLKSLDEHEQQQAAHGLQVGIAMDEHRPEF
                     GQGDFLIRGLLGADRQSGSLAIADFVQEGQTIQFHVRDAHAASEDFHLLLSKERVTHP
                     DSPPKGALLFSCNGRGRRFFETPHHDVATLQHQMGAIPIAGFFAGGEIGPVSGKNFLH
                     GYTASLALFYEEVSRPSKMDMTSDTFNTSKERGS"
     gene            2997..3458
                     /locus_tag="PKM72_12050"
     CDS             2997..3458
                     /locus_tag="PKM72_12050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNP61566.1"
                     /translation="MKNGMTLQSGLRLPVFIIGSWIILLIIFVLNPLSGKAAEETMVT
                     TTSGLQYVDLAPGSGREAHAGETAIVHYTGTLTDGTKFDSSKDRNEPFSFRLGAGRVI
                     KGWDEGVEGMKIGGIRKLVIPPQLGYGSRGAGSAVPPNATLIFEVELLDLR"
     gene            3458..4555
                     /gene="gcvT"
                     /locus_tag="PKM72_12055"
     CDS             3458..4555
                     /gene="gcvT"
                     /locus_tag="PKM72_12055"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNP61567.1"
                     /translation="MDETPFADAHRALNAKLVDFAGWLMPLQYTGVLEEYHAVRKAAG
                     FFDVSHMGRIEISGDQAEAFLQWISTNDVSRLQIGQAQYGMVCQSSGGILDDVFVYKT
                     GSFTFLVCVNASNREKVLQWFIKHQPEKFPGAIVRDRSEELAQIAIQGPASKAIMQKL
                     LGAQIDGLKPRRCLALEGMEFSGLLSRTGYTGELGYEWYVPAAQASLAWDRLLKAGAE
                     FEAKPAGLGARDLLRLDVGYLLYGNDMDENTSPLEANAEWVVAWDKGEFQGHLALGKQ
                     KKVGLTRQLAAFEMVERGIPRHDMAIFSNGQSVGRVTSGNFSPILQKGIGLGYVPPAL
                     CKEGMLLEIDIRGKRVQAKIVTLPFYKRPKT"
     gene            4586..>5076
                     /locus_tag="PKM72_12060"
     CDS             4586..>5076
                     /locus_tag="PKM72_12060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNP61568.1"
                     /translation="MSTEHIYAGKVLILNRETVFLPNGQSTQLEIIRHPGASAVVPIK
                     GDGTVILIRQFRHAAGGFIYEIPAGKLHPQEDPRLCAARELEEEIGYQAGQLELLTSI
                     WTAPGFTDEVIHIYRGTDLKPGRQHLDEDEVLEVVEWPLKKAMAGIQDGTIRDAKTII
                     GLQL"
BASE COUNT         1216 a         1212 c         1409 g         1239 t
ORIGIN      
        1 cctttgagtg gagcgattgg cgaaacggga atttttggtg gcttcaaagt gtgtatgtgc
       61 atccagacca gcgccaacag catatttttc ggcaacttta tgaatacgtg gaaggccaag
      121 cccatttgaa ccgtgagccc atttgcggat atcgtcttta tgtggaagag aaaaatattc
      181 aagcccacca aacctatgcc aaacttggat ttcaagaaac tgcctaccat atgtatgaga
      241 aagaattttc ctggacgtct ccgtcatttc cccaatcaat cgtataaatg cattttcatt
      301 gttatgggca actgggcttg agaacaggta ggactggcaa tatgcgtttg ttcagatggc
      361 gtcagggcgt aaagacctgc cttttttgca tagtagggct gagctgggca tggttccccc
      421 ctgcggtatt ggccaaccct tcggccgccc tgatggagtt agaaccccgt ccacatgtga
      481 gagatcttgg gattaccatc ggccggtatt ctcctggcga aatgaatgcc atcacggacg
      541 tccaaggggt caaggtgggg catgtcacca tctatgaagg caatggagcc ttgcaggtag
      601 gtcaaggccc tgttcggact ggtgtgactg ttattatccc gcgtgaagat gtctggcatc
      661 acaaagttcc agctggagcg tttgtgttaa atgggacagg ggaaatgacg gggttagcgt
      721 gggtgacgga atcagggttt ttggaatatc ccattgcatt aacgaatacg ttgaatgtcc
      781 ctcgtgtggc agatggtgtc atgagctgga tgatgcaacg ttatcccggt attgggatat
      841 ccgatgatac cctcactcct gttgtggctg aatgcgacga tagtcgactg aatgatagcc
      901 agggaaggca tgtgtcttcg gaagatgtgg tacgcgccct ggagactgcc tcgataggtc
      961 ctgttccaga ggggtcggtc ggggcaggga cggggatggt gtcctatcaa tttaaaggag
     1021 ggattggtac cgcctctcga atacttcctt ccgaagaagg aggatatcgt gtcggggtgt
     1081 tggtgaatgc caaccatggt cgtcggcatg aattcatgat ctcgggagta cctgtaggcc
     1141 aagtctatga tacggacctg gcaaccgttt cccaacaaaa ctcgcaagaa tcgaccatgg
     1201 cgactcctgg cgaaatccca gcagatttcc cgcaaaaacg acaggacagc ggatccatca
     1261 taatcatcat tgcgacggat gctcccctcg atgcacgtca actgtccagg ctctcgcgcc
     1321 gggctaccat aggattggca cgaaccgggt caatctccca tcatggcagt ggagattttg
     1381 ttttggcgtt ttccaccggt aatgtcattc ctcattatcc ggaagaacgg acatttttta
     1441 tggcccatct tgctgatacg catataaacc ccttgtttca agccaccgtg gaggccacgg
     1501 aggaagccat tctcaatgcc ttattgcaag ccacaacggt taccgggaga gatggacgac
     1561 gttttgaggc gatctcgatt gaccgactcc gttcgatttt aaatgtttac cgcccaccaa
     1621 ctcggtaagc gactgacctc tagagttgat tttcttgggt cttatcggaa ctgcctaaaa
     1681 taagggcgaa attcaccaac ctttccgcat ggacatcgtt tggcggggat tgctagactc
     1741 aaatccgatt taattacgat gcagtgtcat gttgcccttt cccaagaatc tcatcctgaa
     1801 gtggccgctc atgcggtgac agcatcagtg caagaggcgt taggcgaagc aggttgtcat
     1861 ctcgcatttg tctttttttc cccccatttt tcctcagaca tccagcgcat ggtcgatatc
     1921 atccacgaga cccttcgtcc tcagacattg gttgggtgca tggggcaagg gataattggg
     1981 gccgatcaag aaatagaaga acatccaggt ctggtgctct ggggtcttag aagcaccgac
     2041 atgcgagtgg tcccattcat gttaacgccg aagatagagg gtgaggtagc ttcactagag
     2101 ggctggccgg ttggattaga atccactccc catccgtaca cgtttttcct ctttgctgac
     2161 ccgttttcta cccccattga tgaattattg tccctgttgg aggaacaggc ccctggctcg
     2221 tccgcgattg gaggtattgc cagtgggggc atggatgtcg gggaaagccg cctggtcttt
     2281 aatcgggaca ttattggatc gggggtggtc ggagtggctg ttcagggtgg agtagaaatc
     2341 cggacagtgg tgtcccaagg gtgtcaacct attggggaac gttacgttgt gaccaaggtc
     2401 gatcagaatg tcattcagga gttgggagga atccccccat tggaacggtt ggaatccact
     2461 ttaaaatcgt tggatgaaca cgaacaacaa caagcggcgc acgggctgca agtgggcatt
     2521 gccatggatg aacatcgacc cgaattcggt cagggggact ttttgattcg cggcctgtta
     2581 ggagccgatc gacaatccgg gagtctcgct atcgcggatt ttgtccagga gggccagacg
     2641 attcagttcc atgttcggga tgctcatgca gcgagtgaag acttccattt actcctatcc
     2701 aaggaacggg tgactcatcc ggacagtccg cctaagggcg cgttgttatt tagttgtaat
     2761 gggcgtgggc ggcgattttt cgaaactccc caccatgacg tggccactct gcaacaccag
     2821 atgggggcca ttcccatagc ggggtttttt gcaggtggag aaattggccc cgtgagcggg
     2881 aagaattttt tgcatggcta tacggccagt ctggcgctgt tttatgaaga agtgtctcgc
     2941 ccctcaaaaa tggacatgac atcggatacg ttcaacacta gcaaagagag aggttcatga
     3001 aaaacggtat gacgttacaa tcaggtcttc gattacccgt attcattata gggtcttgga
     3061 tcattctcct catcatattt gttttgaatc ccctgtccgg aaaagccgcg gaagaaacga
     3121 tggtgaccac gacgtctgga ttacagtacg tcgatctcgc tcctggctcc gggcgcgaag
     3181 cccatgcggg agaaaccgca atagtccatt acacggggac gcttaccgac ggaacaaaat
     3241 tcgatagttc gaaagatcgg aacgaaccat tttcatttcg tttaggagcc ggccgcgtga
     3301 ttaagggttg ggacgaaggg gtcgaaggta tgaaaatcgg tggtatccgc aaattagtca
     3361 ttccgcctca gttgggatac ggttccagag gagctggatc agctgttccc cccaatgcaa
     3421 ccctcatttt tgaagtcgaa ctgctcgacc tgcgttgatg gacgaaacac cgttcgctga
     3481 cgcccatcgc gctctcaatg ccaagctggt ggatttcgcc ggatggctca tgccactcca
     3541 atataccggt gtccttgagg aataccacgc tgtcaggaaa gcggccggtt tctttgatgt
     3601 cagtcacatg ggacgcatcg agatatcagg agaccaggcc gaagcctttc tgcaatggat
     3661 cagtaccaat gacgtgagcc gtctccagat agggcaggcg caatatggca tggtttgcca
     3721 atcttcaggg ggcattctcg atgatgtgtt tgtctataaa accgggtcat tcaccttttt
     3781 ggtttgtgtc aacgcttcca atcgggagaa ggtactccag tggttcataa agcaccagcc
     3841 ggagaaattt cctggagcaa ttgttcgtga tcgatcggaa gaactggccc aaattgcgat
     3901 ccaaggtccc gcatcaaaag ctatcatgca aaaactcctg ggggcacaaa tcgacggcct
     3961 gaagccccgt aggtgtttgg ccctggaggg aatggagttt tccggtttgc tgagtcggac
     4021 gggatatacc ggagagcttg gatatgagtg gtatgtgccg gcggcgcaag cgtccctcgc
     4081 gtgggatcga ttactgaagg ccggcgcaga attcgaagcc aaacccgcag gattaggggc
     4141 gcgagatctt ttgcggttgg acgtgggcta cttgctgtat ggtaacgata tggatgagaa
     4201 cacctcgcca ctggaggcca atgccgaatg ggtggtggcc tgggataaag gtgagtttca
     4261 aggacacctc gcgttaggca agcaaaaaaa ggttggcctc acccgtcagc ttgctgcatt
     4321 cgaaatggtg gaacgcggca ttccccgcca cgacatggct attttcagca atgggcagtc
     4381 ggtgggcagg gtcaccagtg gaaatttttc accaatttta caaaaaggga ttggcttagg
     4441 atatgtgccc cctgcgctgt gcaaagaagg aatgttgctc gaaatcgaca ttcgtggaaa
     4501 acgggtacaa gccaagattg tcacactccc gttttacaaa cgtccaaaaa cctaaaacct
     4561 cataatgagt gaaacgggaa actctttgtc gacggaacac atctacgccg gaaaagtctt
     4621 aatactcaat cgcgaaaccg tgtttttgcc caatgggcag tccacccaat tggaaatcat
     4681 ccgccatcct ggcgcctcgg ccgtcgtgcc gattaaaggt gacggtaccg tcatcctcat
     4741 tcggcaattc cgccatgcgg ctgggggatt catttatgaa atcccagcag gcaaacttca
     4801 tcctcaggaa gacccccgcc tctgcgctgc acgagagctg gaggaagaga ttgggtatca
     4861 ggccggtcag ttggagttgc ttaccagtat ttggaccgct ccaggattta cggacgaagt
     4921 catccatatc tatcgcggaa cggatctgaa gcctggccgt caacatttgg acgaggatga
     4981 agtgttggaa gtggtggaat ggccgctcaa aaaggcgatg gccggcattc aggacggcac
     5041 cattcgtgat gccaaaacca tcattggttt gcagct
//
LOCUS       DAORAQ010000178         5064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2378_length_5064_cov_61.779796, whole genome shotgun sequence.
ACCESSION   DAORAQ010000178 DAORAQ010000000
VERSION     DAORAQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2378_length_5064_cov_61.779796"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /gene="queE"
                     /locus_tag="PKM72_12065"
     CDS             <1..626
                     /gene="queE"
                     /locus_tag="PKM72_12065"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNP61569.1"
                     /translation="EIFHSLQGESTRVGQPCVFVRLTGCPLRCTWCDTEYAFHGGTTM
                     PMEEILAQVQSYGCPLVEVTGGEPLHQPGSLVLLTQLCDAGFQVMLETSGAFDIANVD
                     ERVSIILDVKCPGSGMTDRMRWDTLTHLSGKDEVKFVLKDRADYEWARDIVNQYSLGD
                     RCPVHMSPVFGVLHLQSLAEWILEDRLPVRFQLQLHKVIWDPETRGV"
     gene            683..2188
                     /locus_tag="PKM72_12070"
     CDS             683..2188
                     /locus_tag="PKM72_12070"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNP61570.1"
                     /translation="MNILVKSGEATAATTDMLVCLEYEQDKTWSKVVQSVDQKLGGQL
                     STLRKSEEFSGKPNNTALLHIDGKLPAKRVLLVGLGTRETVTLERIRQAMGTAAKRAR
                     TAKAKGIICVMPDVPKSTGPIDDVAQAMVEGFVLGDYRFNEYRTDRTDNNHSLQSCTL
                     LTPSNAHLNEAKDGAKRGQILGEATCFVRDLCNHPANVMTPSRVVTEAKKIAREAKVR
                     LKVLDRKQQEKLGMGGLLGVSRGSIEPPQFIILEYMGGPRTQKPIVFVGKTVTFDSGG
                     ISLKPSENMEQMKADMTGGAEVLATIRAASRLKLPVNAIGLLPVTENMPGGRATKPGD
                     ILTMLSGKTVEVQNTDAEGRLILADGLAYASRLKPACVIDIATLTGAASVALGQFAIG
                     MLGNDDTIKAELKKAGNHAGERVWEMPLWDEYFEQLKSDVADMRNIGGRGGGMITAAL
                     FLSKFVGDHPWVHLDIASTDWGTTERPYVPKGPTGIGTRLLIQYLLNHANR"
     gene            2204..3292
                     /gene="nagZ"
                     /locus_tag="PKM72_12075"
     CDS             2204..3292
                     /gene="nagZ"
                     /locus_tag="PKM72_12075"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNP61571.1"
                     /translation="MSIAELVGQLCMIGFEGTEVTPDLRAWMQEFRPGGIILFSRNLV
                     DPAQIAHLTNDLQALAGDTPLLIGIDQEGGRVSRLPSGFTIFPPASTVAHSGSTELAY
                     QAAAVTAKELRAVGINMNMAPVLDIHTNPSNPIIGDRAFGTEPEQVCLMGAATLAGLH
                     DQRVLACGKHFPGHGDTSTDSHVELPVVHASRERLESVELKPFQYAIAHGLQAMMTAH
                     VHYPALDPTNPATLSSIILTDMLRQQMGFPGVILSDDLEMGAIVNHATIGEAAVRSLQ
                     AGVDMLLVCKTRRLATDTINAVCGAVESNVLDQSHLEASLARIASVKQQFVSPYHPIN
                     VPSILETVGLPAHRHVLAQIQDQFRTLT"
     gene            3558..3959
                     /locus_tag="PKM72_12080"
     CDS             3558..3959
                     /locus_tag="PKM72_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61572.1"
                     /translation="MALKKGDIIDERKRFPDSCGLVLKVMGGGEGFEKIRCCGHDLTQ
                     EDVVPSFNQERGRRAGALAVGMPLNEKIVATDSCGLRLMVMAGGEGFQEVICCGHSFG
                     ASAIQSLEFGQMRGEDTGQEHSPNTPHQGTS"
     gene            3975..4454
                     /locus_tag="PKM72_12085"
     CDS             3975..4454
                     /locus_tag="PKM72_12085"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNP61573.1"
                     /translation="MLGWTPESIKQWVKWMQFLDRWGYITACLSFLLVGMLVFGYSWV
                     AYAQSVQTGFLPATITLLNDLLFVIILLELFRTVLGFLQNDRIRLEPFLHVGIIASVR
                     RILTAGAEFSHQDSVPDDIFRHYLMDMSLHVVVILVLMIALYLHRKSELPVVPVFNK"
     gene            4615..5022
                     /locus_tag="PKM72_12090"
     CDS             4615..5022
                     /locus_tag="PKM72_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61574.1"
                     /translation="MESLPELVGTVHIIMGPYRGNPIALYMCQEESTCHISPRIYPWN
                     QTVGSGDTPNKAAADFEAKWKERDLADDMYTGPHWEGGIKPEKPKPVPPPKPATPPAA
                     KTAAAQDTKTPSPKSPAPPDPPQPAPEAEDAAP"
BASE COUNT         1212 a         1345 c         1299 g         1208 t
ORIGIN      
        1 ccgaaatctt tcatagcctt caaggggaat ccacccgtgt gggacaaccg tgcgtatttg
       61 tccggttaac ggggtgcccc cttcgctgta cctggtgtga cacggagtat gcctttcatg
      121 gtggaaccac catgccaatg gaggaaattt tggcgcaggt gcaatcatat gggtgtccgc
      181 tcgtcgaggt gacgggtggt gaaccattgc atcaacccgg ctctctggta ttgttaacac
      241 aattatgcga cgccggattt caggtgatgc tggaaaccag cggggcgttt gatattgcca
      301 acgtggatga gcgggtttcg attattctgg atgtgaaatg tccggggagt ggcatgacgg
      361 accggatgcg gtgggatacc ctcacacatc tatccggaaa agacgaagtg aagtttgttt
      421 tgaaagaccg ggccgattat gagtgggcgc gtgatattgt gaaccagtat tcattggggg
      481 accgctgtcc cgtccatatg agtccggtct ttggagtcct ccatttgcaa tccctggctg
      541 aatggattct ggaagatcga ctgcctgttc gatttcaact ccaattgcat aaagtgatct
      601 gggatcctga aacccgcggc gtgtaacgca acgggaaaag ggtcgtttta cggtaggggc
      661 gatttatttt ttgaggaggc gtatgaatat tcttgtgaag agtggtgaag ccaccgctgc
      721 aacaacggac atgttggtat gtttggaata tgaacaagac aaaacctgga gcaaagttgt
      781 tcagtcggtc gaccagaaac tcggtggaca attgagcacc ttgcgcaaaa gtgaagaatt
      841 ttccggtaaa ccgaataaca ccgccttact gcatatcgat gggaaattgc ccgcaaaacg
      901 cgtgcttctt gtcggcctgg gaacacggga gacggtgacc ttggaacgaa ttcgtcaagc
      961 gatggggacc gctgccaaac gcgcccgcac cgccaaggcc aaagggatta tttgcgtgat
     1021 gccggacgtg ccaaagagca ccggccctat tgatgacgtg gctcaagcta tggtggaagg
     1081 gttcgttctt ggggactatc ggtttaatga gtaccgcacg gaccgtacgg acaataacca
     1141 ctccttgcaa tcctgcaccc tactcacccc ctccaacgct cacctgaatg aggccaaaga
     1201 tggcgccaaa cgtggccaaa tccttgggga ggcgacctgt ttcgtccggg atttgtgcaa
     1261 ccaccccgcg aacgtgatga cgccttcccg ggtcgtcacc gaagccaaaa aaatcgccag
     1321 ggaggccaaa gtccggctca aagtcttgga ccgcaaacag caggaaaaac tcggcatggg
     1381 cggattattg ggggtctctc gaggcagcat cgaaccgcct caattcatca ttctggaata
     1441 tatgggcggc cctcgcacac aaaaacccat tgtctttgtc ggcaaaaccg tgacatttga
     1501 tagcggtggg atctccctaa aaccatcaga aaacatggaa cagatgaaag ccgacatgac
     1561 cggtggtgct gaagtcttgg cgacgattcg agccgcctcc cggctcaaac ttccggtgaa
     1621 tgcgatcggc ctcttgcctg tgacggaaaa catgccgggg ggacgagcta ccaaacccgg
     1681 cgatatcctt accatgctct ccggaaaaac ggtggaagtc cagaataccg atgcggaagg
     1741 tcgcctgatt cttgctgacg gcttagccta tgcctcacgg ctcaaacccg cctgcgtgat
     1801 tgacatcgcc acactgaccg gtgcggcctc tgtcgcgttg ggacagttcg cgattggcat
     1861 gttgggtaat gacgacacta taaaggccga actcaaaaag gccggcaatc atgccggaga
     1921 acgggtctgg gaaatgcccc tgtgggatga atattttgag cagcttaaaa gtgacgtggc
     1981 ggacatgcgg aatatcggtg ggcgcggagg cggaatgatt accgcggcac tattcttatc
     2041 aaaattcgtc ggagaccatc cctgggtcca tttggacatt gccagcaccg attggggcac
     2101 aaccgaacgt ccctatgttc ctaaaggtcc gactggtatc gggacccggt tgctcattca
     2161 gtatttacta aatcatgcca atcgctaggt ggccatacac gcgatgtcta tcgctgaact
     2221 cgtagggcaa ctatgtatga tcgggttcga agggacggag gtcactccgg accttcgagc
     2281 ctggatgcag gaattccgac cgggagggat cattcttttt tcccgaaacc tggttgaccc
     2341 tgctcaaatt gcacacctca cgaacgacct ccaagcactt gccggtgaca ccccgcttct
     2401 catcggcatc gatcaagaag gtgggcgcgt ctcccgcctt ccttcaggct ttaccatctt
     2461 ccctcctgca tcaaccgttg cccactcggg atccacggaa ctggcctacc aagccgcggc
     2521 cgtaactgct aaggagttac gcgccgtagg gattaatatg aatatggccc cggtcctgga
     2581 cattcataca aatccctcca atcctattat cggagatcga gcgtttggga ccgagccgga
     2641 acaggtgtgc ctcatgggag cggccactct tgccggcctg cacgaccaac gcgtattggc
     2701 ctgcggaaaa cattttcctg gtcatggcga taccagtacg gattcacatg tggaactgcc
     2761 ggtggtccat gcctcccggg agcgactcga aagtgtggag ctcaaacctt ttcaatacgc
     2821 cattgctcac ggtcttcagg ccatgatgac ggcccatgtc cactatcccg ccctcgaccc
     2881 cacaaatccc gcaaccctct cttccattat tttgacagac atgcttcggc agcagatggg
     2941 atttcccggt gtcattttaa gcgacgactt ggaaatgggc gccattgtga accatgcgac
     3001 aatcggcgag gcggctgtac gctcgttgca agccggagtg gatatgttat tggtgtgcaa
     3061 aacccgcaga ctcgcaaccg ataccattaa cgctgtttgt ggtgccgttg aatccaatgt
     3121 gttggatcaa agccaccttg aggctagcct ggcccgcata gcctccgtca aacagcaatt
     3181 tgtctctccc tatcatccaa tcaatgttcc ctccattctt gaaaccgtcg gccttcctgc
     3241 tcatcgccat gtgttagcac agatccaaga ccaatttcgc actttgacct gaatgcacag
     3301 aaagtttaga gacggattac catccatact ccaataggcg gtcgaggctt cgtctccttc
     3361 tgggccactt tatggatgcc ggccactatc gctcaactga ccaactgaca aaagatggcc
     3421 ataacccaat tctggacatt ccctacaaag atttgtttag ctaataacct tgctgtataa
     3481 accgacacat ccaaacctct attcctattg ctgatgacag aatttcatga ttcatttcga
     3541 aagcaaggag catggccatg gcattgaaaa aaggggacat aatcgacgaa cgcaaacggt
     3601 ttccggattc ttgcgggctg gtactcaagg tgatgggcgg aggggaaggt tttgaaaaaa
     3661 ttcggtgttg cgggcatgac ctgacccagg aagatgtagt tccctcgttt aatcaagagc
     3721 ggggccgcag agcaggagcc ttggcagtcg gcatgccact caatgagaaa atagttgcga
     3781 ctgattcctg tggacttcga ctaatggtga tggcgggagg tgagggcttt caggaagtca
     3841 tctgctgtgg tcattcgttt ggggcgagtg caattcagag tttagagttt ggtcagatgc
     3901 ggggagagga cacggggcag gaacactccc ccaacacccc ccatcaggga acctcctaag
     3961 aggacacatt cgacatgtta gggtggacac cagaatcgat caagcaatgg gtcaaatgga
     4021 tgcagttcct cgaccggtgg ggctatatca cggcctgcct gagttttctc ctcgtgggca
     4081 tgctggtttt cggttatagt tgggtggcct atgctcaatc ggtacagacc gggtttttgc
     4141 cggccaccat caccttactg aatgacctgt tgttcgtcat cattcttttg gagttgtttc
     4201 gcacggtttt aggcttcttg caaaacgacc gtattcgcct cgaacccttc ctccatgtag
     4261 ggattatcgc ctccgtccga agaattctca ccgctggagc tgaattttcg caccaggatt
     4321 ccgtccctga tgacatcttt cgtcattatc ttatggatat gtccctgcat gttgtcgtga
     4381 ttttagtcct catgatcgca ttatatcttc accgaaaatc cgaactcccc gtagtgcctg
     4441 ttttcaacaa atagcccttc cgagttccag taatattctg tttcttattc atcattccta
     4501 catcaaatta ttgtcaaaaa tacctgatga acttctttca ttcatattgc ataccgccga
     4561 gggctgtggc agtatagcct cctttttgga caacaatcgg ttgtgttggt catcatggag
     4621 tctcttcctg aattagttgg cacggtccat attatcatgg gcccttatcg aggaaatccg
     4681 attgcactat atatgtgcca ggaggagtcc acctgccaca tttctcctcg catctatcca
     4741 tggaatcaaa ctgttggatc tggagatacc cccaataaag ccgcagcaga ctttgaagcc
     4801 aagtggaaag aaagggacct ggccgacgac atgtataccg gccctcattg ggaaggtggc
     4861 atcaagccag aaaaacccaa acccgttcct cctcctaaac ccgccactcc tcctgcagct
     4921 aagaccgcgg ccgcacaaga caccaaaact ccctcaccta aatctccagc tccgccggat
     4981 ccacctcaac cagccccaga agctgaagac gcagctccgt aagactccgt cctcttcgcc
     5041 ataccaggta ttctctttcg taaa
//
LOCUS       DAORAQ010000179         5048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2390_length_5048_cov_64.700781, whole genome shotgun sequence.
ACCESSION   DAORAQ010000179 DAORAQ010000000
VERSION     DAORAQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_5048_cov_64.700781"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKM72_12095"
     CDS             <1..341
                     /locus_tag="PKM72_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HNP61575.1"
                     /translation="GLALFTGMAYLLDPLFHLLGLTVLTAPSLEGFWTSLYQSVWWRL
                     EHFNNSIVMGSLVFSVAMFVPVLLLSNLLIRRYRQHVLAWVQKTRIMQMFKASKLYQT
                     YETFSSWRPG"
     gene            341..2131
                     /locus_tag="PKM72_12100"
     CDS             341..2131
                     /locus_tag="PKM72_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HNP61576.1"
                     /translation="MTKWIRWWGLGAFVVFAGIFGCVWIFLVDGWVKSLIEGAGTEAV
                     GAQVELDAADLSLFPAGLSLTRLQVTNPRKPMTNAVEVANVTMGLDGLNLLRRKVIVE
                     EMALEGVRLGTLRTTSGAVKPTPDVSAEAESGMFSLALPALEVPDVKNILEKEDLETI
                     RLIEALKGDVEREKEAWEARLKELPGKAQLAEYKERVKSLKKAAKGGLEGVLGGVGEV
                     QVLKQDIEQEIESLKGAKKEFEEKLALLRTRLDQVKTAPQRDIQHLKAKYNLSPKGLA
                     HVSQTLLGPQIGSWVHQGAAWYERAKPFLEGDRAGGGEDGPQVDKPLRGKGVDVHFQE
                     VHPLPDFLIRLTKVSISLAVGDLAGTIHNITNDQPTLGQPITFVFSGDRLKGVQSISL
                     EGILNHVVPADSKDHLTVQAKGYELDNLALSKNDKWPVTLASGVGGVNVNADLKDRGL
                     AVHGIGNFHSLHFSAGKDGDTNPLTKSLSSAVAGVSEFSVQANVTGTLERHEVTVSSD
                     LDGVLQDAAGKMVNELAAKFGTELQLAISAKIAEPMQQLQNNLGGFEKIAGQLTERVT
                     QHDAVLKSLLEKSLPIKGLKDLPGGLKLPF"
     gene            complement(2151..3569)
                     /gene="sthA"
                     /locus_tag="PKM72_12105"
     CDS             complement(2151..3569)
                     /gene="sthA"
                     /locus_tag="PKM72_12105"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNP61577.1"
                     /translation="MSSSITTPTQFQYDLICIGSGPAGQRAAIQAAKIGKRVAVIEKK
                     RTVGGACLETGTIPSKTFREAVMSFTGPHQHWGRSPDTAKIRPTADQLVARVAEVIRT
                     EGEVMNDQLRRNDVELIQGEATFVDPHTLLVHTDNQSQTLSSAHILIAVGTIPTPPPN
                     IPIDGQYIFDSDGIIHLQQLPRTMTVVGAGIIGIEYASMLGALGVEVTLVDSRIRPLE
                     FLDGEIVDELIYQMRNHQVTFRLGETVEKLEITDTPSRKVVIHLASGKRLVSGLALYS
                     VGRTGATATLQLEKAGLTVDARGRLPVDSQYRTPVPHILAAGDVIGFPSLAATSSEQG
                     RLAACYAFGLEANPMTSLFPVGIYAIPEISMVGATEEQLTLQKIPYEVGIARYREIAR
                     GTIMGDLNGMLKLLFHRNDHRLLGVHVIGTGATELIHIGQAILHTERGLEYFLTTVFN
                     YPTLAECYKVAALDAYNKLCET"
     gene            complement(3864..4829)
                     /locus_tag="PKM72_12110"
     CDS             complement(3864..4829)
                     /locus_tag="PKM72_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNP61578.1"
                     /translation="MTQHHDQEAFAKLVDRIGPEHAGQRMEMQAHYSALLLKGYHLHI
                     HMEEFPAIAWLIKTLLKTTGIWPLAVKNTSQYRIVEHTVSIPHLPESFEGFRIVHLSD
                     LHIEGMIDKGHALQAAMSRLQYDLCVMTGDFRFLTYGHYDKTLILMEELVKTLKAPYG
                     VTGILGNHDWLEMVPGLERCGIRMLLNEAQSLEKGSDAIWLLGLDDVHYYETGDLEKT
                     VRLAPTDAVRILLVHSPEIIPEASNAGMDLYLCGHSHGGQICLPGGNPIITHCRCPRS
                     YKAGPWQYRSMQGYTSRGVGTSLFPIRLFCFPEIIIHTLTRAPQS"
BASE COUNT         1216 a         1141 c         1441 g         1250 t
ORIGIN      
        1 tggggctggc gctgtttacc gggatggctt atctccttga tccgctcttt catctgctgg
       61 ggttgaccgt tctgaccgct ccatcgttgg aagggttttg gacctccctt tatcaatcgg
      121 tgtggtggcg attggagcat ttcaataatt ctattgtgat gggcagcctg gtgttttctg
      181 ttgctatgtt tgtccctgtt cttctgcttt cgaacctctt gattcgtcgc tatcgacaac
      241 atgtcctagc ctgggtacaa aaaacccgga ttatgcaaat gtttaaagcc agcaaattgt
      301 atcagaccta tgaaaccttt tcgtcatgga ggcctggctg atgacgaaat ggattcggtg
      361 gtgggggcta ggggcgtttg tcgtgtttgc aggcattttc ggttgcgtgt ggatattttt
      421 ggtggatgga tgggtgaaaa gtttgatcga aggggcaggg actgaagccg tgggagctca
      481 ggtagagctg gatgcggctg acctcagctt gtttcccgct gggctctcgt tgaccagact
      541 gcaagtgaca aatcccagga agcccatgac caatgcggtg gaagtggcca acgtgactat
      601 gggtttagat ggactgaatc tcctacggcg aaaagtcatt gtggaagaaa tggctttgga
      661 aggtgttcgt ttgggcactc tcagaaccac atccggcgcc gtcaagccaa ccccagacgt
      721 gtctgctgaa gcggagtccg ggatgttttc tcttgcgctt ccggctcttg aagttcctga
      781 cgtgaaaaat attttagaaa aagaagacct ggaaaccatc cgattgattg aggcattgaa
      841 aggggatgtt gagcgggaaa aagaggcatg ggaggcaagg ttgaaagagt tgccggggaa
      901 agcccagttg gccgaataca aagaacgggt gaaaagtctg aaaaaggctg caaaaggcgg
      961 actggaaggc gttttgggcg gagtggggga agtgcaggtc ttgaaacagg atattgagca
     1021 ggaaattgaa tcgctcaaag gggcgaagaa ggagttcgag gagaagttgg cgttactacg
     1081 cacacgcctc gatcaggtga aaaccgcacc gcaacgggat attcaacacc tcaaggccaa
     1141 atacaattta tccccaaaag ggttggctca tgtgagtcaa acattattgg ggccccagat
     1201 tggatcatgg gttcatcagg gagcggcctg gtatgaacga gcgaagccgt ttctggaagg
     1261 ggaccgcgca gggggaggag aagatggccc acaggtcgac aagcctttgc gtgggaaggg
     1321 tgtggatgtg cattttcagg aagtgcatcc gcttcccgat ttcctgattc gtctgacgaa
     1381 ggtatcgatc agtttagcgg taggtgattt ggccgggacc atccataata tcaccaacga
     1441 ccaaccgaca ttgggacaac ccataacgtt tgtgttttcc ggagaccggt tgaagggggt
     1501 acagtctatt tcgttggaag ggattctcaa ccatgtggtg cccgccgatt ccaaggatca
     1561 tctgacagtg caggcaaaag ggtatgaact ggataatctg gcattatcca aaaatgataa
     1621 atggcccgtc acactggcta gcggggtggg tggtgtcaac gtaaacgcgg atctcaagga
     1681 ccggggatta gcagttcatg gcatagggaa tttccacagt cttcacttct cggcaggaaa
     1741 agatggtgat accaatcctt taaccaaatc cctcagctct gccgtggcgg gtgtatctga
     1801 gttttctgtt caggctaatg tgacggggac attggaacga catgaagtca cggtatcctc
     1861 tgatctggat ggtgttcttc aggatgcggc tggcaagatg gtgaatgaat tggctgcaaa
     1921 gtttggcact gaacttcaat tggccatctc ggcgaagatt gccgaaccga tgcaacaact
     1981 tcaaaataat ttgggcgggt ttgaaaaaat tgccggtcaa ttaaccgagc gcgtcacgca
     2041 gcatgatgct gtgctcaaaa gtcttcttga aaaaagcctt cctatcaaag gattaaagga
     2101 tcttcccggc ggactcaagc taccgtttta accgatacgt gaaaagtctg ctatgtttca
     2161 caaagtttgt tgtaggcgtc gagggccgct actttgtagc attccgccag cgtgggataa
     2221 ttaaagacgg tcgtgaggaa gtattcgagc ccacgttcgg tgtgaaggat ggcctgtccg
     2281 atgtgaatga gttctgtggc tcccgttccg atcacatgta cgcccaagag ccggtgatcg
     2341 tttcgatgaa ataacagttt caacatcccg ttcaaatctc ccatgattgt gccacgagcg
     2401 atttcccggt aacgggctat tcccacctca tacggaattt tctggagggt cagttgttcc
     2461 tctgtggctc ccaccatcga aatttccgga atggcataaa taccaacagg gaacaggctt
     2521 gtcatggggt tggcttccag accaaaggca tagcaggccg ctaagcggcc ttgctcggaa
     2581 gatgtggcgg ccaagctcgg aaaaccgatg acatcacctg ccgccagaat atgcgggact
     2641 ggggtgcgat attgagaatc gaccggcagg cggccgcggg catctacagt caatcccgcc
     2701 ttctcgagct ggagtgttgc tgtggctccc gttcgtccga cggaatagag ggcaaggcct
     2761 gagaccagtc gtttcccgga agccaaatgg atcacgactt tccgggatgg tgtgtccgta
     2821 atttctaatt tttcgacagt ttcaccgaga cgaaaagtga cctggtgatt ccgcatttga
     2881 taaatgagct catcaacaat ctcaccatcg agaaattcca agggacgaat tcgtgaatcc
     2941 acaagggtga cttcgacgcc aagtgcccct aacattgagg catattcaat accgatgatc
     3001 ccggctccaa ccacggtcat ggttcgtggg agctgttgca ggtggatgat gccatcactg
     3061 tcaaaaatat attggccgtc tatgggaata ttgggaggag gtgtcgggat ggttcctacc
     3121 gcaatcagaa tgtgggcgct cgacagggtt tgtgactggt tgtcggtgtg aaccaacagg
     3181 gtgtggggat ccacaaaagt ggcttcccct tggatgagtt ccacatcgtt tcttcgtaat
     3241 tgatcgttca tcacctcacc ttcggtgcga atgacctccg ccacgcgcgc gaccaattgg
     3301 tcggccgtgg gtcgaatttt cgctgtatcc ggtgaacgac cccaatgttg atggggtccg
     3361 gtaaaggaca tgacggcttc tcgaaaggtt ttactgggaa tggtcccggt ctctaaacag
     3421 gcaccgccca ctgtacgctt tttttcaatg accgcgaccc gtttccctat tttggcggct
     3481 tgaatcgccg cccgttgacc cgctggtccg ctcccgatac aaatgaggtc gtattgaaat
     3541 tgagtgggtg tagtgattga ggagctcatg aggataagac gaggcaggat gccgttatgg
     3601 atgctccctc agagcatagc gtgagaaaag agggaagtac agatgaaatt taacattgat
     3661 tcgactgcct cacgattcta cagaaattgg atgaggagtc tgcgagtaat ctcgggcaga
     3721 gatagcctgc gtctaaggtg cgattatcaa cattctccgg gaaaagacat atcctcaacc
     3781 atgggtgctg gttgtcaccc ttctacttct ttcgtcagtt ttcccgatgg agaagatcag
     3841 tgtatgaatt gaccaggcga ttttcaagat tggggagctc gtgtgagcgt gtgaatgatg
     3901 atttcgggaa agcagaagag gcgaatggga aatagggacg tgccgactcc tcgagaagtg
     3961 tatccttgca ttgaacggta ttgccatggg cctgccttat aggacctggg gcaacgacaa
     4021 tgggtgatga tgggattgcc cccaggtaga cagatttggc ctccgtgcga gtggccgcag
     4081 agatagaggt ccatgccggc gttcgaggct tcgggaataa tttccggcga atgcactaag
     4141 aggatacgca cggcgtcggt gggtgcgagg cgtacggttt tctccagatc gcccgtttcg
     4201 taataatgga catcatccaa cccgaggagc caaatcgcat ccgacccctt ttccagggat
     4261 tgcgcttcat tgagtagcat tcggattcca catcgctcga gcccaggcac catttcgagc
     4321 caatcatgat tccccaaaat gcccgtgacc ccgtaaggag ccttgagggt cttcaccaat
     4381 tcctccatga gtatcaacgt cttgtcataa tggccatagg tgagaaagcg aaaatcaccg
     4441 gtcatgacgc acaggtcgta ctgcagcctg gacatggcgg cctggagcgc atggccttta
     4501 tcgatcatcc cctcaatatg aagatctgac aaatgcacaa tgcgaaatcc ctcaaaggat
     4561 tctggcaggt gagggatgga gacggtgtgt tcgacaattc ggtattgtga cgtattcttc
     4621 acagctaatg gccaaatacc ggttgttttt aagagagtct tgataagcca ggcaatggct
     4681 ggaaattcct ccatgtgaat atggaggtga tagcctttta agagaagggc cgaatagtgg
     4741 gcctgcatct ccatgcgttg gcctgcatgt tcaggcccga tacggtccac taattttgca
     4801 aaggcttctt ggtcgtggtg ttgggtcatg tgcttgtcag tcaagtggga tcagaggtac
     4861 cattgccatc atgcaacgac tagatctctt tcaacccggg ttgcctgata tccagtttgt
     4921 tctcatggtt ttggcattgt gtactgcaga gctggagacc atgaatgcgc caggatccgt
     4981 gagagagacc gtgttcaatc ggtgttgggc attaatgcat gaaagccctc cgcctgtgaa
     5041 gacacaag
//
LOCUS       DAORAQ010000180         4918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2464_length_4918_cov_59.180136, whole genome shotgun sequence.
ACCESSION   DAORAQ010000180 DAORAQ010000000
VERSION     DAORAQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_4918_cov_59.180136"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /gene="lysS"
                     /locus_tag="PKM72_12115"
     CDS             complement(<1..1450)
                     /gene="lysS"
                     /locus_tag="PKM72_12115"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNP61579.1"
                     /translation="MEELNEQRQQRLEKLHALQDGGLRPFGTRFPTTHTLETILQSYS
                     DLTKEELEGTPISCTVAGRIVAMRRFGKAAFTALQEEGHRLQVYLKKDLLGDQSYELS
                     QSFDLGDWIGVEGKLFRTKTDELTVEVHTLTFLTKGLRPLPEKWHGLTDIETRYRQRY
                     VDLIANPSVHQVFRTRSRIINTIRSFLTEREYLEVETPMMHSIPGGATARPFVTHHNT
                     LDVDLYLRVAPELYLKRLIVGGFRRVFEVNRNFRNEGISTIHNPEFTMLEFYQAYADY
                     HDLMDLTEQLFHTLSLEVCGGPTVDYQGQSISLAPPWRRLPYLDSIVEVNQLDPEVLS
                     DHKKAVDAAKRLGVDCAPQASHVDILVNLFEATVEPTLIQPTFITDFPIEISPLARKK
                     ESNPALTDRFELFIGGRELANGFSELNDPLDQRQRFEAQVALRDAGDDEAHYLDEDFI
                     RALEYGMPPTAGEGIGIDRLVMLLTNQASIRDV"
     gene            complement(1649..2771)
                     /gene="prfB"
                     /locus_tag="PKM72_12120"
     CDS             complement(join(1649..2701,2703..2771))
                     /gene="prfB"
                     /locus_tag="PKM72_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNP61580.1"
                     /translation="MLEDIRIRMEKINDTILEFRRYLDVPRLTTELEELERETSQPDF
                     WNNPRQAAKAGRRQSEIERQLARLHQLDQQRDDLLAILDLIGQQGDPELEAEWSKGIK
                     DIEATLEAWRLEQLLSDEHDQNNAILSINPGAGGTESQDWAQMLMRMYLRWAQERGYK
                     VETIDLQPGDEAGIKSVTLAIGGPLAYGYLHSEAGVHRLVRISPFDANKRRHTSFAAV
                     GVYPELDDDIEIEIDDKDLKIDTFRAGGAGGQNVNKVETAIRMTHLPTGTVVQCQNER
                     SQLQNRIGAMRVLKAKLYELEQQKKDAEFQNIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTNHESGNVAAVMDGALDSFIEAYLTMKASKASHMPAN"
     gene            complement(2792..4420)
                     /gene="lnt"
                     /locus_tag="PKM72_12125"
     CDS             complement(2792..4420)
                     /gene="lnt"
                     /locus_tag="PKM72_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNP61581.1"
                     /translation="MGQTIPPFSFPSRRAGALFFALAFIGILHPLSFPPFDLGWLAWL
                     VLIPLHMIIHDLPPRRALFYGWLSGTIAFAGTVTWVITAMHQFGQVPFIVSALLMLLL
                     AAYLGLYMGIYGWGYAKFQRTCPNLLWLGAPALWVALEFVRTYALSGFPWALLGYSQY
                     QWLAVIQFADVTGVYGVSFLLVMGNVALTSLLLWIYAKTRGLPLPGPWVSVAGFGCAL
                     SLVLAYGAWRMQEQANLDASARTLSIGLVQANIDQAVKWNEAYRDETLRRYTSLSQQA
                     GKNTDLLIWPEAATPFLFEREPAYQSQVLDIAKDTHSSLLFGSPTLRFQQDGRPYLYN
                     SAFLVTAEGRVSDRYDKRHLVPFGEYIPLRSILFFLDKLVVGIGDFKSGQGVMTMQVP
                     TVPPGSGPRFGVAICFEVIFPDLVRRMAREGANFLVTITNDAWFGDSAAPYQHFGMVV
                     FRAVENHLAFARAANTGISGFIAPDGHILSQTSIFSEQAVMGLVPLRSSSPTFYTQFG
                     DVFSWGCVIMAGILFTWCRFTTPLSTRKPRSSPI"
     gene            complement(4638..4829)
                     /gene="rpmB"
                     /locus_tag="PKM72_12130"
     CDS             complement(4638..4829)
                     /gene="rpmB"
                     /locus_tag="PKM72_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNP61582.1"
                     /translation="MAFSCDVCGKNRLVGNNVSHANNRTKRIFRPNLRTVRALVKGAA
                     QRIKVCTRCLRSGLVQKAV"
BASE COUNT         1198 a         1255 c         1318 g         1147 t
ORIGIN      
        1 tgacatcgcg gatcgaggcc tgattggtga ggagcatgac gagtcgatca atcccaatgc
       61 cttctcctgc ggtgggaggc atgccatatt caagggctcg gataaaatcc tcgtccagat
      121 aatgggcttc gtcgtctccc gcatccctca aggccacctg ggcttcaaat cgttgtcgct
      181 ggtccaacgg atcgtttaac tccgaaaatc cattcgctaa ttcccgtcca ccgataaaga
      241 gttcaaagcg gtctgtcagg gccgggttgg attctttttt tctggccaag ggggaaattt
      301 cgatagggaa atcggtaatg aatgttggtt gaatcaatgt tggctcgaca gtggcttcaa
      361 agaggttgac gagaatgtcg acgtggctgg cttgcggagc acaatcaaca cctaaccgtt
      421 ttgccgcgtc cacggctttc ttgtggtcgg acaagacctc agggtctaac tggttgacct
      481 ctacgatcga gtccaaatag gggaggcgac gccagggggg cgccagggaa atgctttgcc
      541 cctggtaatc taccgtcgga cctccgcata cttccagact caaggtgtga aacaactgtt
      601 cggtcaggtc catcagatca tgataatccg cataggcctg ataaaattcc aacatggtaa
      661 attccggatt atgtatggtg gaaatgcctt cattgcggaa atttcggtta acttcaaaga
      721 cgcgccggaa tcccccgaca atgaggcgct tgaggtataa ctcgggtgcc acacgcaaat
      781 ataagtccac gtcgagggta ttatgatgag tcacaaacgg gcgtgctgtg gctccgccag
      841 ggatggaatg catcatcggc gtttccactt cgagatattc ccgttccgtg aggaacgaac
      901 gaatggtatt gatgattcga ctgcgcgtgc ggaatacctg gtgcaccgat ggattggcaa
      961 tcaaatccac atagcgttgc cgataccggg tttcgatgtc ggtgagtcca tgccattttt
     1021 ccggaagagg ccgcagtcct ttcgtgagaa aggtgagtgt atgcacttcc accgtgagtt
     1081 cgtcggtttt ggttcggaat aacttgcctt caactccgat ccaatcccct aaatcaaagg
     1141 actgcgaaag ttcgtaggac tgatccccaa gcagatcttt cttaagatag acctggagac
     1201 gatggccttc ttcctgtaag gccgtgaagg cggctttgcc aaaacggcgc atggccacaa
     1261 tgcgtccggc caccgtgcac gaaatcgggg tcccttcaag ctcttctttg gtcaagtccg
     1321 aatacgattg aagaatggtc tcaagcgtgt gggtggtggg aaaacgtgta ccaaagggac
     1381 ggaggccacc atcctgaaga gcgtggagtt tttccagccg ttgttgtcgt tgttcgttta
     1441 attcttccat aacttacatg gctctactga ttcatgaagg tgaacattct cttatattcc
     1501 tctggtgagg ttcgaccact catatgttaa ggaaacggcc aaatgattga aatatttaac
     1561 aacccctcga tggttgtcta gccagcctat ggagcgcgaa tatgttcggt gatgaatcat
     1621 gaaggccgag tatcggatgt ggatgctgtt aattggccgg catgtgtgaa gctttcgagg
     1681 ccttcatcgt taagtacgct tcaatgaaag agtccagcgc tccatccatg accgcagcga
     1741 cattgcctga ctcatggttg gtccggtggt ccttgaccat ttgatacggt tggaacacat
     1801 aggagcgaat ttggctaccc caggcaatat ctttcttctc cccgacaata ttttgaaatt
     1861 cagcgtcctt cttttgttgt tccagctcat acagcttggc ttttaacacc cgcatggcgc
     1921 cgatccggtt ttgcaattgc gaccgttcat tttggcattg caccacggtt cctgtgggaa
     1981 gatgggtcat gcgaatagcc gtttccacct tgttcacatt ttgcccgcct gccccgcccg
     2041 cgcgaaaggt atcgattttc aaatccttat cgtcaatttc aatttcaata tcgtcgtcca
     2101 attccggata aacccccact gcggcgaacg aggtatgtcg acgcttgttg gcgtcaaagg
     2161 gggagatccg aaccagccgg tgcacgccag cctctgaatg caggtatcca taggccaatg
     2221 gaccgccaat ggccaaggtt acgcttttga tccccgcttc atcgcctggt tgcagatcga
     2281 tggtttccac tttgtatccg cgttcctgtg cccatcgaag atacatgcgc atgagcatct
     2341 gtgcccagtc ctgggattcg gttccccctg ctccgggatt aatgctgaga atggcattat
     2401 tctggtcatg ttcgtccgat aacagttgtt ccaagcgcca ggcttccagc gtggcctcaa
     2461 tgtctttaat gcccttgctc cactcggctt ccagttccgg atcgccctgt tggccaatca
     2521 gatcaagaat ggcgagaagg tcatcccgtt gctggtcgag ttggtgaagg cgtgcgagtt
     2581 ggcgttcgat ctcggattga cgtcgtccgg ccttggcggc ctgacggggg ttgttccaaa
     2641 aatccggttg ggaggtctcc cgctcgagtt cttccagttc agttgttagc cggggaacgt
     2701 caaagatacc tccggaattc cagaatggtg tcgttgattt tttccattcg aatgcgaata
     2761 tcttcaagca tgggcagcac tcctctcgtg gctatatcgg tgaagaccgt ggtttgcgcg
     2821 tggaaagcgg tgtggtaaac cgacaccaag taaacaaaat accagccatt ataacacaac
     2881 cccaactaaa gacatcccca aattgcgtat aaaaggtggg tgatgaggag cggagtggca
     2941 ctaatcccat caccgcctgt tctgagaaaa ttgatgtctg ggaaaggatg tgtccgtccg
     3001 gggcaatgaa gccggaaatg ccggtgttgg ctgctcgggc aaaggccaaa tgattttcca
     3061 cggcacgaaa gacgaccatg ccgaaatgtt gatacggcgc agcagaatcc ccgaaccagg
     3121 catcattggt aatcgtgacg agaaaattag caccctcccg ggccatccgg cgaaccagat
     3181 cggggaaaat cacttcgaaa caaatagcca ccccgaatct cggaccactg ccggggggca
     3241 ccgttgggac ttgcatggtc atgactccct ggccggattt gaagtcgccg atccccacca
     3301 cgagcttatc cagaaaaaag agaattgacc gcaagggaat gtattcccca aagggcacca
     3361 gatgccgctt atcataccgg tctgagaccc gaccttcagc cgtcaccaga aaggcactgt
     3421 tatacaaata cggccggccg tcttgttgaa atcgaagcgt ggggctgcca aatagcaatg
     3481 agctgtgcgt atcttttgca atatcaagga cctggctctg ataggcgggt tcccgctcga
     3541 agagaaacgg tgtggccgct tccggccaaa tgagcagatc ggtattcttt ccggcttgtt
     3601 ggctgagcga ggtgtagcgt cggagtgtct catcccggta ggcctcgttc catttgacgg
     3661 cttgatcaat attggcttgg accaacccaa tcgacagcgt gcgtgccgag gcgtcaaggt
     3721 tggcttgttc ctgcatccgc caggctccat aggccaaaac gagactcagc gcgcagccaa
     3781 agcctgctac ggacacccag ggtccgggaa ggggcaagcc acgtgttttg gcgtaaatcc
     3841 acaagaggag ggaggttagc gcaacatttc ccatgacgag taaaaatgac acgccataga
     3901 ctcccgtcac gtcggcaaat tgaataaccg caagccattg gtattgggaa tagcccaaga
     3961 gcgcccaggg aaacccgctt aacgcatagg ttcgcacaaa ttccaaggcg acccacaagg
     4021 cgggggctcc cagccacaag aggttgggac acgtgcgttg gaacttggca tatccccagc
     4081 catagatgcc catgtatagg ccaagataag cagccaaaag gagcatcaac agggcactta
     4141 cgatgaacgg cacttgacca aattgatgca tggccgtgat gacccatgtc acggttcccg
     4201 caaaggcgat ggttcccgac agccatccgt aaaacaacgc ccgtcttggc gggaggtcat
     4261 gaatgatcat gtggagggga atcaacacca accaggccag ccaaccgaga tcaaagggcg
     4321 ggaagctcag aggatgaagg ataccgatga aggcgagagc gaaaaaaagg gcccctgccc
     4381 tgcgagacgg aaaggaaaaa ggtggaatgg tctgccccac gtcaaaaggg acccacggtg
     4441 gttttcaggg gcgagccctg ctgtcgtatc aggtgcggcc gtatggtcaa tccggaataa
     4501 tacccaggaa tcagtagaga tttcggctgg aaagggaaga agtaatgata acagtcgaac
     4561 tgatgaacac gattcagggc tacaagaacg ccaaatggga cataccgttc acctcgcatg
     4621 aacggcccta ggaacgacta gacggctttt tggaccaatc cggaccgcag gcaacgggtg
     4681 cagaccttga tgcgttgagc ggcacctttc accagtgcac gtaccgttcg cagatttggg
     4741 cgaaaaattc tttttgtccg attattcgcg tggctgacat tatttcccac taagcggttt
     4801 tttccgcaca cgtcacatga aaaagccatg gtcttctccc tggatattta aaatgaccga
     4861 attgtcgatt agagtctgtt tgttaccaca ggtgccattg tacacacaag tacccgaa
//
LOCUS       DAORAQ010000181         4896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2477_length_4896_cov_63.356744, whole genome shotgun sequence.
ACCESSION   DAORAQ010000181 DAORAQ010000000
VERSION     DAORAQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_4896_cov_63.356744"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="ald"
                     /locus_tag="PKM72_12135"
     CDS             complement(<1..964)
                     /gene="ald"
                     /locus_tag="PKM72_12135"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNP61583.1"
                     /translation="MVIGVPKEIKDHEFRVALTPHGVKELCGRGHRVVIQEGAGEGSG
                     FGDDLYRQAGAVLAPTPQATYGDAQLIVKVKEPHSEEWPFLRKDHVVFTYLHLAASKA
                     LTEALLNIGCAGIAYETTEDAKGQFPLLYPMSEIAGQMSVQIGAHYLEKQRGGSGVML
                     GGVAGVPPGEVVILGSGVVGASAVKIALGMGAKVTVLSLDLWQLRMLYDRYPGRLVTV
                     ASNQSMIHHFLTLADLVIGAVYLPAARTPRVITRSMISDMKPGSVLIDVSVDQGGCAE
                     TTHPTTHSDPVYVVDGVLHYAVANIPGIVPYTSTLALTNATLPFI"
     gene            1169..1513
                     /locus_tag="PKM72_12140"
     CDS             1169..1513
                     /locus_tag="PKM72_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNP61584.1"
                     /translation="MSKFTHHIFVCVNERPKGDPRGCCAALGSVGLHGFFKKEVERLG
                     LKGIVRANKAGCLDQCEYGPSVVIYPEGVWYWVGQEADVTEIMERHIGKGEIVERLLM
                     PGKNGSLPTVKS"
     gene            1588..2022
                     /locus_tag="PKM72_12145"
     CDS             1588..2022
                     /locus_tag="PKM72_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61585.1"
                     /translation="MGNEEKWKANLRKVAFLKSFPGWISSWEQGIGATIEQVLPIPGH
                     APHTVLLLSEGRFVVTAPVHDEPQMVTAGLMSARPHLESIHAGAFTEYDHLTRLDQDL
                     GRMARLENILNAIDNNLDRIPELKTRIQELVKQWEKENHQSQ"
     gene            2019..2297
                     /locus_tag="PKM72_12150"
     CDS             2019..2297
                     /locus_tag="PKM72_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61586.1"
                     /translation="MNMLEMFSGALFEGVKPMMVRRDHLVRHPDRCTHNSICIPICPT
                     GAWLSTPPYKFDSSRCMESCRLCLDACPTQAIYGVFLKGEKLLSPKQE"
     gene            complement(2299..3342)
                     /locus_tag="PKM72_12155"
     CDS             complement(2299..3342)
                     /locus_tag="PKM72_12155"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNP61587.1"
                     /translation="MRLSPSDQHLLGDVLKRVSRSFYLTLAILPRAVRSQIGLAYLLA
                     RAADTIADTGDLEDGVRLECLRLLKGQLWGSVPDLAQIKKIQGQVLTQQSNPDERRLL
                     EELEGCFRIYQKFSPTDRSQIAQVLSVLIGGMQFDLHQFPQKTDGEAVRALQAIHDFE
                     YYTYAVAGCVGEFWTKMVCSHLPGFRGWDQACMIPLGIRYGKGLQMVNILRDLPRDLR
                     NGRCYIPTVLLDQAALAPHQLLDESSVQAFRPLFRKLIQEARDHLDQGWRYTMAIPRM
                     EVRLRLACMWPILIGIRTLQQLSVSPLVLSPNSPIKIARSDVYQIVASTGLTGGCGTV
                     GTAYWGYWRKRIV"
     gene            complement(3348..3785)
                     /locus_tag="PKM72_12160"
     CDS             complement(3348..3785)
                     /locus_tag="PKM72_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNP61588.1"
                     /translation="MGIHSEFYYTRLILGLVLVMGYWSLFDGLYAQASESSLSLVEEP
                     FGQQVMISMESYAFTPSEFVVKAGTPVTLTLSNQSFLVPHNFLLDGPSGIRLVDVDIS
                     SGDTKAVTLTLTEPGIYPFYCDKQLLFFPTHREQGMEGRLIVR"
     gene            4010..4894
                     /gene="htpX"
                     /locus_tag="PKM72_12165"
     CDS             4010..4894
                     /gene="htpX"
                     /locus_tag="PKM72_12165"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNP61589.1"
                     /translation="MKKFKGIGLLLIANILIFITLSISFTVLSEMILPAFGIDLRGSL
                     AQQDLLWAFVIGFGGAFISLAFSKQMARAFIDCTQITEPRSQAERLVYDTVQKISEKL
                     SISMPEVWIYESPDPNAFATGPTKNNSMVAVSTGLLNNLNEGEVRAVLAHEMGHVYNG
                     DMFTTTVLAGLMNTFVYFISRFIYRQVAERNEALGFGVYIVLQIVLSVLAMIPISWWS
                     RRREFAADRFAANTVGKEHMISALEGIDRWVQRTQFDYSSQDALATMKISGKSHSFMQ
                     IFATHPPIEARVNALRQL"
BASE COUNT         1303 a         1264 c         1134 g         1195 t
ORIGIN      
        1 ctataaaagg tagcgtggcg ttggtcaagg ctaaagtcga tgtatacggc acaatcccag
       61 gaatatttgc gacggcatag tgcaggactc catcgacaac ataaaccgga tcgctatgcg
      121 tggttggatg ggtcgtctcc gcacacccac cctgatcaac tgaaacatca atcagaactg
      181 atccgggttt catgtcagaa atcatagatc gcgtaatcac tcgaggcgtt ctcgccgcag
      241 gaagatacac ggccccaatg acaagatccg cgagggtgag gaaatggtga atcattgact
      301 gattggacgc aaccgtgaca agacgaccag gataccgatc atacagcatg cgtaactgcc
      361 ataaatccag acttaacacc gtgactttgg ctcccatgcc taaggcaatt ttcacggctg
      421 atgcgcccac aaccccggat cccagaatca cgacctctcc cgggggaacg ccggccaccc
      481 cacctaacat gaccccggaa cctccacgtt gtttttccaa ataatgtgcc ccaatttgga
      541 ccgacatttg accggcaatt tcactcatgg gatacaagag cggaaattgc ccttttgcat
      601 cctcagtggt ttcgtaggcg atgcccgcgc acccgatatt tagcaaggcc tccgtcaagg
      661 ctttggaggc ggccaaatgc aggtaggtaa agacaacatg atcttttctt agaaatggcc
      721 attcttcgga atgcggctct ttcaccttca caattagttg ggcatctccg taggtagctt
      781 gtggggttgg agccagaacc gcaccagctt gccgatagag atcatcccca aacccactcc
      841 cctccccggc cccctcttga atcacgaccc gatgcccacg gccacacaat tccttcacac
      901 catgaggagt cagtgctact cgaaattcat gatccttaat ttcttttggg accccaatca
      961 ccataatctt gtcctcttcc cctctctgca agccttgcct ccggcctgca ccctggtgaa
     1021 tctatcaggt acattttgtt caatgaatac tagggttctc ccgtcaatat tgcccgatga
     1081 cctggacagc ttcctgaacc ctcaggtaag atcggctctt tgttttccaa tttccttgtt
     1141 cggtcatttt aatgaaaatt taaagattat gagtaaattt acccatcata ttttcgtgtg
     1201 tgtcaatgaa cggccaaagg gagatccgcg agggtgttgt gctgccctgg gttctgtcgg
     1261 attacatggg ttcttcaaaa aagaagtcga gcggttaggg ttaaaaggta ttgtccgggc
     1321 aaacaaagcc gggtgtctgg accaatgtga gtatggacca agcgtcgtca tctatcccga
     1381 aggggtctgg tattgggttg gacaggaagc cgatgtcacc gaaatcatgg agcgacatat
     1441 cggcaaagga gaaatcgtgg aacgactcct gatgccagga aaaaatggtt cattacccac
     1501 agtcaaatcc taaggttttg tcttctcaca aagaaaccac tggtcttata accctggcgc
     1561 cacaacctgg cacatggtct tcatcttatg ggcaatgaag agaaatggaa agccaactta
     1621 cggaaagtag cctttttaaa gtcctttccc ggctggatct cctcctggga acaggggatt
     1681 ggagccacca ttgaacaggt ccttcccatt cccgggcatg ctccccatac cgtcctcctt
     1741 ttatctgaag gccgctttgt tgtcaccgca cccgtgcatg acgagccaca aatggtcaca
     1801 gcgggactca tgtccgctcg tccacatctt gagtccattc atgccggcgc gtttacggaa
     1861 tatgatcacc tgacccgtct ggatcaggat cttggacgca tggccaggtt agaaaatatt
     1921 ctgaacgcca ttgataacaa tcttgatcga attcctgagc tcaaaacgcg cattcaggaa
     1981 ttggtcaaac aatgggagaa ggagaatcat caatcacaat gaatatgctt gaaatgtttt
     2041 ccggagccct ttttgaagga gtgaagccta tgatggtgag aagagaccac ttggtccgac
     2101 atccggaccg ttgtactcat aattcgattt gcattccaat ttgtccgacg ggagcctggc
     2161 tctcgactcc tccctacaag ttcgattcgt cacggtgcat ggaatcgtgc cgactctgtc
     2221 tggatgcctg tccaactcag gccatttacg gagtctttct caaaggggaa aaacttctct
     2281 caccgaaaca agaataaatt agacaatacg cttcctccaa tatccccaat aggccgtacc
     2341 cacagtgcca cagccacccg tgagtcccgt ggaggccacg atttgataga catcgctccg
     2401 tgcaattttg atgggtgagt tcggactcag cacgagcggt gacaccgaca attgttgcaa
     2461 agtacggatc ccaatcaaaa tcggccacat gcaggccaaa cgcaggcgaa cctccatgcg
     2521 cggaatggcc atcgtatagc gccaaccctg atctaaatga tcccgagcct cttgaatcaa
     2581 ttttctgaag agggggcgaa atgcctgaac agaagattca tcgagtagtt gatgaggagc
     2641 aagagccgcc tgatccagca acaccgtcgg aatatagcac cgcccattcc ttaaatcccg
     2701 tggcaaatcc cgaagaatat tcaccatctg caatcccttc ccatagcgaa tgcctaaggg
     2761 gatcatgcag gcctgatccc acccacgaaa tcccggtagg tgactgcata ccatcttcgt
     2821 ccaaaattcc cccacgcagc ccgcaaccgc atacgtgtaa tattcaaaat cgtgaattgc
     2881 ctgtaatgct cgtaccgcct ccccatccgt tttttgagga aattggtgga ggtcaaattg
     2941 cattcctcca atcagtactg aaagaacctg cgcaatctgg gaccggtcgg ttggactgaa
     3001 tttttggtaa attcggaaac acccctctaa ttcttccaat aagcgacgct catcgggatt
     3061 cgattgttgg gtaagtactt gcccctggat ttttttgatc tgcgccaaat cagggacgct
     3121 tccccacaac tgccccttta aaagacgtaa acattccaga cggaccccat cctctaagtc
     3181 ccctgtatcg gcaatagtgt ccgcagccct ggccagcaag taggccagac caatttgcga
     3241 acgaacggca cgaggcagaa tggcgagggt taaataaaag gagcgggaaa cccgtttgag
     3301 cacgtcaccg agaagatgtt gatccgatgg agaaaggcgc atgagagcta gcggacgatg
     3361 agacggcctt ccatcccttg ctcacgatgc gtgggaaaga acaggagctg tttatcgcaa
     3421 taaaatggat agatgccggg ctcggtcaag gtaagggtga cggccttagt gtcgccgctg
     3481 gaaatatcca catccaccag acggatgccg ctaggaccat ccagcaaaaa attgtgagga
     3541 actaaaaatg actgattact cagggtcagt gtcaccggtg tacccgcttt gaccacaaat
     3601 tcggatggcg taaaagcgta actttccata ctgatcatca cctgttgtcc gaagggttcc
     3661 tccaccaaag atagggaaga ctctgaggct tgagcgtaaa ggccatcgaa aagactccaa
     3721 tagcccatca ccagaacaag acccaatatc aatcgtgtat aataaaattc agaatggata
     3781 cccatgaaaa atgatctctt catccggaaa gatatcataa cggtttttca tccgagggtc
     3841 acgtctttcc cactcttgac tctcaaggaa aaggtgttat cttccaacat gttgaaccct
     3901 aacagtcatt ttttggacaa tcgtgaagag aacaggaaaa tctttcatga agtcgaacac
     3961 attagcgttt ttccaccaat ggatgtggaa caattaaggg agattgtcta tgaaaaaatt
     4021 taaaggaatt ggtttattat taattgccaa tatcctcatc tttatcaccc tctcaatctc
     4081 gttcaccgtt ttatcggaga tgatcctgcc ggccttcggg attgatctgc gtggatcact
     4141 ggcccaacag gacctgttat gggcctttgt catcggattt ggcggtgcct ttatcagtct
     4201 ggccttttcc aaacaaatgg ccagggcttt tattgattgc acccaaatta cggaaccccg
     4261 ttcccaggct gaaagactcg tgtatgacac tgtccaaaaa atatctgaaa agttaagtat
     4321 ttcgatgccg gaggtctgga tttatgaatc accggatccc aatgcgtttg ccaccgggcc
     4381 aacaaaaaac aactccatgg tggccgtctc aaccggtctg ctcaacaatc ttaatgaagg
     4441 tgaagttcga gccgtcctgg ctcatgaaat gggccatgtc tacaacgggg atatgtttac
     4501 caccaccgtg ttggcaggct tgatgaatac atttgtctac tttatcagcc ggtttatcta
     4561 tcggcaggtg gcagaacgga atgaggctct aggatttgga gtatatatcg ttttgcaaat
     4621 cgttctctcc gtcttggcga tgattccgat tagctggtgg tcccgccgtc gggaatttgc
     4681 agcagaccga ttcgcagcca acacggtagg caaagaacat atgatctctg ctctggaggg
     4741 cattgatcgt tgggtgcaac gtacccaatt tgattatagc tcccaagatg ccctggcaac
     4801 gatgaaaatt tctggcaaat cacacagttt catgcaaata tttgccactc atccgccgat
     4861 tgaagcgaga gtgaatgcat tacggcaact ctaatt
//
LOCUS       DAORAQ010000182         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2494_length_4877_cov_65.158855, whole genome shotgun sequence.
ACCESSION   DAORAQ010000182 DAORAQ010000000
VERSION     DAORAQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_4877_cov_65.158855"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..620
                     /locus_tag="PKM72_12170"
     CDS             339..620
                     /locus_tag="PKM72_12170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNP61590.1"
                     /translation="MAKARARHILVSTEEACNNLKTQIENGGDFAAAAKEHSTCPSGK
                     QGGDLGEFGPGQMVKEFDTVVFSGELGKVHGPVKTQFGYHLLEITSRTP"
     gene            867..2501
                     /gene="aepX"
                     /locus_tag="PKM72_12175"
     CDS             867..2501
                     /gene="aepX"
                     /locus_tag="PKM72_12175"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HNP61591.1"
                     /translation="MPSEHQPSKSTQFKKLLLSPQLEFICEAHNGLSAKIVEEAGFHG
                     IWASGLSISAQFGVRDNNEASWTQVLDNLEFMSDAARIPILLDGDTGYGNFNNMQRLI
                     RKLEQRHIAAVCIEDKLFPKTNSFLKGDTQPMADMEEFCGKIKAGKDAQSDPDFCIIA
                     RVEAFICGWGLAEALRRAEAYRQAGADGILIHSALAVPEEILAFKREWSNRSPVVIVP
                     TKYYSTPTDVFRQHGFSMVIWANHMLRAAVATMQTTARVLKEQENLLSIEDNIVPVSE
                     IFRLQGAAELMEAEQRYLPKSADRASAIVLAASRGDELGELTEDKPKTMVNIRGVPIL
                     SHIVDAYNTVGIKNIAVVRGYKKEAVNLVNLTYVDNNDFATTGELNSLFKALQVKGKH
                     DQDIIISYGDVLFNSYIPQALLQEKDDFVIFVDSDWRGKTSYTRLGGFAECTAPNSRK
                     AFNAQIHLKQLGRAVPSNMIHGVWMGFFKVSAQGVAQLHEILTELLADPIHRKAGMAT
                     LFQELLRRNYSIRVLYTVGHWLDINSLDDVVEAGSF"
     gene            2577..2780
                     /locus_tag="PKM72_12180"
     CDS             2577..2780
                     /locus_tag="PKM72_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61592.1"
                     /translation="MKLSDLSQNEFKELVNSMVDDRLCELLGDPDLGLALDDKVRAQL
                     KQVLESKERVTGDNVAERLGIRW"
     gene            2787..3074
                     /locus_tag="PKM72_12185"
     CDS             2787..3074
                     /locus_tag="PKM72_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HNP61593.1"
                     /translation="MAWYRVSYLPSVLDDLQATDQSMAQRLLDKTKWLASNVANLRHE
                     PLHDDLPGLIQYAVGDWRILYSIDRDEQIVHIYRIATRKELYAGRTAQQQS"
     gene            3071..3400
                     /locus_tag="PKM72_12190"
     CDS             3071..3400
                     /locus_tag="PKM72_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61594.1"
                     /translation="MNKSEQIEYELTKAAMGLQDGNDGLARVCARRAVALASQHWAEE
                     RNLPTWKGDAMHHLRQIQGEKTLPVSVRAAAQRLLTKVTEQTQLPMTTDPITDARIIL
                     DHLNAQI"
     gene            3558..4397
                     /locus_tag="PKM72_12195"
     CDS             3558..4397
                     /locus_tag="PKM72_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61595.1"
                     /translation="MADPRLLMGAASPQFIMEAPESLEPVAAQLKDINPQSLQRMMDF
                     MGLQHPGPPIRVILAPNGSPLAQETPAWMSGYAMGGASTIVLLTDRSLTYPNNSLNDV
                     FLHEIGHILAHRAAGEHPLPRWFDEGLAMMTARRWDLEDRARLVWAMVSDTQISLNDL
                     NTLFVKDEASVRRAYVLAYAFTLDLLEHTGQDTPKRILAKVRQDIPFSEAFAQTTFMT
                     LRQAEEQFWSRQTLWSRWIPVATSSGMVWLAITLLAFWAFTRQRKRAAAIKRQWKEDD
                     FDL"
BASE COUNT         1271 a         1283 c         1175 g         1148 t
ORIGIN      
        1 aattttgatg aattggtttt ggatttcatt tgtgattatc tggcgatgaa tcccgacaac
       61 taatctaacg actcaatcat tacctaccgt tctttcacat cccgtgctct tctgccaagc
      121 actttccccc ttacaccggc cttcagtctc acacgttgac cgggcctcgt ccatccgact
      181 cattcctgaa taccgacaca ttcgcgcttc taccaaacgc ttcaggatcc ggagatttca
      241 ctggtttcat agatagattc tcatgaaatt tgacctcgac tggtcaatca tgggaaaaag
      301 gctattgttc aaatttttag cctaaaagga gaatgctcat ggcaaaagcg cgtgcccgtc
      361 acattttggt ttcaacagaa gaagcatgca ataacttaaa aacacaaatt gaaaatggag
      421 gagattttgc cgccgccgct aaggaacatt ccacctgtcc atcaggcaag caaggtggag
      481 acttgggcga gtttggcccc ggtcaaatgg tcaaagaatt tgatacggtc gtatttagtg
      541 gcgaactcgg caaagtccac ggccctgtca aaacccagtt tggctatcat cttcttgaga
      601 tcaccagccg cactccataa atcgttcact attcatagga caagggcatg gtctcgtggc
      661 tatgccctta tcttatggct ccaccaccct caatcacagt cacaagtttt gggcccatga
      721 ctgcccacac gttcatgcaa cagatgccaa ggccaaaggt attcccggct tccttcagat
      781 cgggaaaggt ttggcatcaa gattcccctt gatgtagact ccagtacatc attgagcaac
      841 cagaaactct gtcacatgta ctgattatgc caagcgaaca ccagccttca aaatccacgc
      901 aatttaaaaa gctcctcttg tctccccagc ttgagtttat ctgtgaagcc cacaatgggc
      961 tcagcgccaa gatcgtggaa gaagcgggat tccatggcat ctgggcgagc ggtctctcca
     1021 tttcagctca attcggcgtg cgggataata acgaggcgag ttggactcag gtcctggaca
     1081 atctggaatt tatgtctgac gcggctcgca ttccgatact ccttgatgga gatactggct
     1141 acggcaactt taataatatg cagcgtctca ttcgtaaatt ggaacagcgg catatcgcgg
     1201 ccgtgtgcat tgaagacaag ttatttccaa agacgaatag ttttctcaag ggtgacacgc
     1261 agcccatggc cgacatggaa gaattttgcg gaaaaattaa agccggaaaa gatgcccaat
     1321 cagatccgga tttctgcatc atcgcccggg ttgaagcgtt catttgcgga tggggactgg
     1381 ctgaagcctt gcggcgggca gaggcttatc gccaggccgg ggctgatggc atcctgattc
     1441 acagtgcact ggcggtgcct gaggagattc tggcattcaa acgggaatgg agcaatcgat
     1501 cacccgtggt gattgtgccg acaaaatact actcaactcc aaccgatgtg ttccggcagc
     1561 atggcttttc catggtcatt tgggcaaacc acatgctccg cgccgccgtc gccaccatgc
     1621 aaacgacggc ccgcgtcctc aaagaacaag aaaatctgtt atcaattgag gataacattg
     1681 tgccggtctc agaaatcttt cgtctgcaag gtgccgctga actcatggaa gccgagcaac
     1741 gctatcttcc aaaaagcgcc gaccgggctt cagccatcgt cctggccgcc tcccgtggag
     1801 atgagctggg ggaactgacg gaagacaaac caaaaaccat ggtaaacatt cgcggagtcc
     1861 cgatcttatc acacattgtg gacgcctata acacggtagg aatcaaaaac attgccgttg
     1921 tccgaggata taaaaaagaa gccgtgaatc tggtaaattt gacctacgtg gataacaatg
     1981 actttgccac aaccggcgaa ttaaactccc tcttcaaagc gcttcaagta aaaggcaaac
     2041 acgatcagga catcatcatt tcctatgggg atgtcctatt caattcctat atcccgcaag
     2101 cgctccttca ggaaaaagat gactttgtga tttttgtgga cagtgactgg cgaggcaaaa
     2161 caagttatac caggcttggt ggatttgccg aatgcaccgc tccaaactcc cgaaaagcct
     2221 ttaacgccca gattcatctc aaacaattag gcagggcagt tccttcgaac atgattcatg
     2281 gcgtatggat gggatttttt aaagtctcgg ctcagggtgt ggcgcaatta catgaaatcc
     2341 ttaccgaact gcttgccgac ccgattcacc ggaaagcagg tatggcaacc ctgttccagg
     2401 aattattacg aagaaattat tcgataaggg ttctctatac cgtcgggcat tggctggaca
     2461 ttaacagcct ggatgatgtg gttgaggccg gaagctttta atgaggatct gaaaatcttc
     2521 ctctattctc ttgaacaata acatgctcgg taattcccac aaaggaagcc ctcacaatga
     2581 aactttcaga tctctctcag aacgaattca aagaactggt gaatagtatg gtggacgacc
     2641 ggctatgtga actgctgggc gatccggacc ttggcctggc ccttgacgat aaggtgcgcg
     2701 cgcagttgaa acaggtcttg gagtccaagg aacgggtgac aggagacaac gtcgccgaac
     2761 gcctcggaat ccgctggtga cataacatgg cttggtatcg tgtcagctat ctgccctcag
     2821 ttctggacga ccttcaagca accgaccaga gcatggctca gcgtttgttg gataagacca
     2881 agtggctggc ctccaatgtt gccaatcttc ggcatgaacc tctgcacgac gacctccccg
     2941 gtctcatcca atatgcagtc ggtgactggc gcattctcta ttccatcgac cgggacgagc
     3001 aaattgtcca catttatcga attgcgacca gaaaagaact ttacgccggt cgcactgcgc
     3061 agcagcagtc atgaataagt ctgaacagat cgaatacgaa cttaccaagg ctgccatggg
     3121 gctccaggat gggaacgatg gtttggccag agtgtgcgca cgacgagccg tggcactggc
     3181 ctcgcagcat tgggcagagg aacgaaatct tccgacctgg aagggagatg ccatgcatca
     3241 ccttcgtcag atccaaggag aaaagacttt acctgtatct gtgagagctg ccgcgcaacg
     3301 actcctgact aaagtgaccg aacaaactca gttgcccatg accacggatc ccatcaccga
     3361 cgcgcgcatc attcttgatc atctgaatgc acagatttga acccaggtca tcggaaaacc
     3421 atccaggttc tcaagctttc ctcactcccc tcgtcgagcc cctgtgtgtt gataccattc
     3481 aacatagttt gcacaagtcc cataggttca ttaggaggtt tggacttcag cttctgttcc
     3541 tgacgatcat tgtcataatg gccgaccctc ggctactcat gggagcggct tccccccaat
     3601 tcatcatgga agccccggaa tcgttggaac cagtagctgc ccaactgaaa gacatcaatc
     3661 cacagtctct tcagcgcatg atggacttta tggggctcca acaccccgga ccaccgattc
     3721 gggtcatatt ggcgccaaac ggatcgcccc ttgcacagga aacgccagca tggatgtctg
     3781 gttacgcaat gggaggggct tccaccattg tgctcctcac cgaccggagt ttgacttacc
     3841 cgaataattc actcaatgat gtcttcttgc atgaaatcgg ccatatcctg gcccatcggg
     3901 cagccggaga acaccctctg ccccggtggt ttgatgaagg attagcgatg atgaccgcac
     3961 gaaggtggga tttagaagat cgagccagat tagtctgggc gatggtttca gacacgcaaa
     4021 tttctctgaa tgatctcaac accctcttcg tcaaagacga ggcctccgtc cgacgggcct
     4081 atgtcctggc ctacgccttt accttagacc ttctggaaca cacaggccag gataccccaa
     4141 aacggatttt ggcaaaagtt cgacaagaca tccccttttc tgaagccttt gctcaaacca
     4201 cctttatgac actcagacaa gcagaagaac agttctggag ccggcagacg ctctggagtc
     4261 gctggatccc ggtggccacc agttccggaa tggtctggct cgcgatcacc ctcctggcct
     4321 tttgggcgtt taccagacag cggaagaggg ccgcagccat taagagacaa tggaaagaag
     4381 atgacttcga tctatgatag gctttattac ggccatcaca tcggtcttct tgaccgtttc
     4441 ggcttcgatt gagaggtgag aatgcaaatc tataggcatt tccctttctt tccgactgat
     4501 accgaaggaa attaaccata ttctcaagca cacttcgtca acgggaattt tttgaattca
     4561 tgaatgttgc cgggtttcgg cccggcagcc gaggtccttt tgtttcggca aaaggaccca
     4621 aaaccattga cgccccgtcc ggcctcataa gagtggacgg acgcaaactt ggggagggcg
     4681 gaccaactcg ccgggctcaa acaaggtccg ccggttcatg agagcgtcct ccctttggcc
     4741 agccggcagg cgtcacagca gcggcgaggg agacacacgg ataaaaagag gtctttggaa
     4801 agtaattgga atcactatac attatcaggc aacaaagata gcccgtcttg aaatcttccc
     4861 tacaccttga aaggaac
//
LOCUS       DAORAQ010000183         4873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2497_length_4873_cov_65.203196, whole genome shotgun sequence.
ACCESSION   DAORAQ010000183 DAORAQ010000000
VERSION     DAORAQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2497_length_4873_cov_65.203196"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKM72_12200"
     CDS             complement(<1..217)
                     /locus_tag="PKM72_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61596.1"
                     /translation="MDNVFAPRVLRELKGEPGEIASYRQEGGYDAWVQCVTKNDPQTI
                     IAQLKEAHLRGRGGAGFPTGLKWDKVVN"
     gene            complement(490..1437)
                     /gene="mdh"
                     /locus_tag="PKM72_12205"
     CDS             complement(490..1437)
                     /gene="mdh"
                     /locus_tag="PKM72_12205"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNP61597.1"
                     /translation="MSRIKVTVVGAGNVGGSIAQRLAEKNWYDIVLVDIVEGLPQGKA
                     LDLLEAGPICAYDSALTGANHYEASKDSDIVVITSGVPRRPGMSRDELLETNTNIVSG
                     VVRETAKRSPNAILIIVSNPLDVMTHVAHRVSGFPRNRVIGMAGVLDSARFRSFIAEA
                     LQVSVENIHAMVLGGHGDSMVPLNRYTTVAGRPVTEWLSPEALEALIKRTREGGIEIV
                     NLLKTGSAYYAPAASVVEMVEAISKDQKKILPCAALCAGEYGFHDLFLGVPVKLGSGG
                     AEEIIEYALTPQEQAALAVSAKAVGELCGQVDLMMKKTT"
     gene            complement(1480..1890)
                     /locus_tag="PKM72_12210"
     CDS             complement(1480..1890)
                     /locus_tag="PKM72_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61598.1"
                     /translation="MTSLPSHPDVPPWLYKLFTGHQYPYVRRQAKFDRKDRQEDGERF
                     EPTQDDIREKFWEIFPRCTTKMLQEVKVGMIVAFKELGGYEAGTYQEFIDNPDEFLSR
                     EYGKKKIKVNFYDGDNFVCTINFKVAGWTSHEDD"
     gene            complement(1908..>4873)
                     /locus_tag="PKM72_12215"
     CDS             complement(1908..>4873)
                     /locus_tag="PKM72_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNP61599.1"
                     /translation="IELKEISSKIQGEAVWALGELNRKGCVASVIPWLDLGITFAQAS
                     GALGLRYFKESPMILGILEGESNRDALLAHVLELADGSNEAAPQCAYEWFKVLPQLCG
                     EMAIPELQEWARLGIDLSEWNYVLGNEFFRECPSIARAVPLESARSWIGFGMKLMVQN
                     SLGKPDYIGTLEFFRTSPSLFLEFNDANVKQRVIDLGTRLADHSPEQAIAFLAKATEV
                     LPGVSTVEWKIRILKFGLLVADRDPEATLAYFARVSEVVGLAGKEDDTGVFDAWFAQG
                     MEALEYSVEAGRAFFGLETQQACSAVEQAMSGVSLRQVARSLKMFARALCGEDVAIEG
                     LPETGGVSVGTSHMSASPVSEKAQVSTDGKTVYLPLVMRRSESREGNRRWYTVMVAHE
                     VGHVEFGTYALSTQVLQRVATGVQTRYEKEPPSQKRGVHTLGNLFQLYPQPEIIRDLW
                     EIVEDARIDYLLRHEYPGLQEDLTSLTKEAMELRTLSHGMTAREIVLDALLLLYAGFT
                     KEDFTRPGLQEVVDEIWQIARTILHPTATVDESVELADRLYQELERRIGTLEKDNQQP
                     EPFSNTTEVSDSGGQPEAAEHLEEAYQPLSNWGYRGILNPDHVKGGEEEEQASKGQTE
                     GQQDQATQIGGNGGGDSPSQFERRHPHQPDSSQDPKKPVFGESPMQQWFQPTLRPGDG
                     QQGSRLREGEYLYQEWDGTVRDYRPQWCRVIEQTGREGSPDFVDETFQTYGPIVRLIR
                     RYFETIRPEAFRRMGRQSHGEDIDLDALVNWMVDRRQGNDSSDQVYATRQKRDRQVAV
                     AFLVDMSGSTGRQIGARARPVIDIEKEGLLLLSEALSAIGDQYAMYGFSGQTREAVDI
                     QVLKDFDQPSGGRVGLKISGVTPKQQNRDGAAIRHATHRLKQQPAKVRLLILISDGKP
                     LDDDYADEYSLEDTKMALREARLQGVHPFCITIDQAPTEYVKRMYGEIGYVVVDEVES
                     LPMKLPKIYQRLTAK"
BASE COUNT         1167 a         1439 c         1066 g         1201 t
ORIGIN      
        1 gattgaccac cttgtcccat ttcagtccgg taggaaatcc agcgcccccc cgtccccgaa
       61 gatgggcctc ttttaattgt gcgataatgg tctgcggatc gttcttcgtg acacactgaa
      121 cccaggcatc atatcccccc tcctggcggt acgatgcaat ttcacctggt tcacccttta
      181 actcccgcaa tacccgtggt gcaaatacgt tatccaccga agcccccttg atcaatggtt
      241 tgggcccata tgcatatatc catgattttc attatttgga ttacggtatt ttttacaaat
      301 cccttcctcg aaaaaccaaa tttccttcca tattcccaag ggaaattagg cgtcaaggtg
      361 ggtcaagagc acgagcacga ctcactatac gaacgggtcg ctaaagtcgt caaggtaaca
      421 agacgatcag aaagcacgcc tatttagtga aggaaaaaat gggcaggttc gagacatgcc
      481 ataggcacat caggtggtct ttttcatcat gagatccacc tgtccacaca attccccaac
      541 agccttggct gagaccgcca gagccgcctg ttcctgtggg gtgagcgcat attcaataat
      601 ttcttccgct ccgccggacc ctaatttcac cggcactccc aaaaagagat cgtgaaaacc
      661 atattccccg gcgcataagg ccgcacaggg aaggattttc ttttgatcct tagagatcgc
      721 ctcaaccatt tccacgaccg aggcagccgg ggcataatag gcactaccgg ttttaagcag
      781 attaacgatt tcgattcctc cctcgcgagt ccgcttaatc agggcctcta gtgcctccgg
      841 cgacaaccat tcggtcaccg gccgccctgc cactgtggta tatcgattca agggcaccat
      901 cgaatcgcca tgtcccccca gaaccattgc gtgaatattt tcaaccgaga cctgcaaggc
      961 ctccgcaata aaggaccgga accgtgcgga atccaacacg cccgccatcc cgatgacccg
     1021 gtttctggga aacccactca cacgatgcgc aacgtgtgtc attacatcaa gagggttgga
     1081 gacaataatg agaatggcat tgggagaacg tttggccgtc tcacggacca cccccgacac
     1141 tatgttagta ttcgtttcca ataattcgtc ccggctcatc cccggccggc ggggcacgcc
     1201 tgacgtgatc accacaatgt cagaatcttt gctcgcctca tagtgattcg ctcccgtcag
     1261 ggcggaatcg taggcacaga ttgggccggc ttcgagcaaa tccaaggctt tgccctgcgg
     1321 cagtccttcg acgatatcga ccaacacaat gtcgtaccaa tttttttctg ccaatcgttg
     1381 tgcgatggat cctccaacat ttcctgcacc caccacagtt actttaatac ggctcatgaa
     1441 accacccatc cctttgaagg ggtcgccagc catgcgggat taatcatctt catggctcgt
     1501 ccaccctgcg actttaaagt tgatcgtaca cacaaaatta tccccgtcat aaaaattcac
     1561 tttgattttt ttcttcccgt attcacggct caagaactcg tccggattgt caatgaactc
     1621 ctgataggtt cccgcctcat accctcctaa ttctttgaag gcgacaatca tgccgacctt
     1681 gacctcttga agcattttgg tcgtacaacg agggaaaatt tcccagaatt tttcacggat
     1741 gtcatcctga gtcggctcaa accgttctcc gtcctcctgc cggtctttcc ggtcaaactt
     1801 cgcctgccgg cgaacataag gatattgatg cccggtaaac aacttataca gccacggtgg
     1861 cacatcaggg tgtgaaggta aactggtcat gtatctccca tatcagttca tttggccgta
     1921 agccgttgat aaattttcgg caacttcatt ggaagtgatt cgacttcatc gacgaccaca
     1981 tacccaattt ccccatacat ccgttttaca tattcggttg gagcttgatc aatagtaatg
     2041 cagaagggat ggacgccttg aagacgagct tcccgaagag ccattttggt gtcttccaac
     2101 gagtattcat ccgcatagtc atcatctaat ggtttcccat cactgatcag aatgagaagc
     2161 cgcactttcg cgggttgctg tttcaagcga tgcgtcgcat gtcggatcgc cgcgccatca
     2221 cggttttgtt gtttgggtgt cactccgctg atcttcaaac cgacacgccc cccggacggt
     2281 tggtcaaaat ccttcaagac ctgaatgtcc accgcttccc gggtttgccc cgaaaagcca
     2341 tacatcgcat attgatcgcc aatcgcggac aaggcttccg agagcaggag caagccttct
     2401 ttttcaatat cgataacggg acgcgctcga gctccgatct ggcgtccggt tgatccgctc
     2461 atatccacca gaaacgccac cgcaacctgt cgatcacgtt tttgtctggt ggcatacacc
     2521 tgatcggacg aatcgtttcc ttgccgtcga tccaccatcc aattcactaa ggcatccaga
     2581 tcaatgtctt ccccatgaga ttggcgtccc attcgacgaa aggcttccgg gcgaatcgtt
     2641 tcaaaatacc ggcgaatgag tcgcacaatt ggcccatacg tttgaaacgt ttcgtccaca
     2701 aagtcgggtg acccttcacg gccagtctgt tcgatcacac ggcaccattg gggtcgatag
     2761 tctcgtaccg tcccatccca ctcttgatag agatactctc cctcacgaag gcgagatccc
     2821 tgttgtccat cgcccggacg aagcgtcggt tgaaaccatt gctgcatagg cgattcacca
     2881 aaaactggtt tcttgggatc ttgcgacgaa tcgggttggt gaggatgtcg acgctcaaat
     2941 tggctgggtg aatctccccc accatttccc ccaatctggg tagcttgatc ctgttgccct
     3001 tctgtctgtc ctttcgacgc ctgctcctct tcctccccgc ctttgacatg gtctggattg
     3061 agaatgccac gataccccca gttactcaaa ggctgatacg cctcttctaa gtgttcagcc
     3121 gcttcaggct gtccccctga atcagagacc tcggtggtat tggaaaaggg ttcaggttgt
     3181 tgattgtcct tctccagagt tcctatccgg cgttctaatt cctgatagag ccggtcggca
     3241 agttccaccg attcatcgac cgtcgcagta gggtgtaaaa tggttcgggc aatctgccag
     3301 atttcatcaa ccacctcttg aagtcccgga cgagtgaagt cctccttggt aaaccctgca
     3361 tagagcaaca acaatgcgtc cagcacaatc tcacgggccg tcattccatg ggaaagcgtg
     3421 cgaagctcca tcgcctcctt ggtcaatgac gtcaaatcct cttgtaaccc cggatattca
     3481 tgacggagca aatagtcaat tcgagcatct tccacaattt cccacaaatc ccggataatc
     3541 tccggttgcg ggtagagttg aaaaaggttt cccaatgtgt gaacgcctct cttctggctg
     3601 ggaggttcct tttcgtatcg ggtctgcacc ccggtagcca cacgttgcaa cacttgggtc
     3661 gagagcgcat aggttccaaa ttccacatgt ccaacctcat gagcaaccat caccgtgtac
     3721 catcgccggt ttccttcccg gctctccgat cgcctcatga ccaggggaag atatacggtt
     3781 tttccatcgg tactcacctg agctttctcg gagacaggac tcgctgacat gtgactggtc
     3841 ccgaccgaaa ctcctcctgt ctcgggaagt ccctcaatgg ccacatcctc cccacacaac
     3901 gcccttgcaa acattttcaa ggatcgggcg acttgacgaa gcgacacacc gctcatcgcc
     3961 tgctctactg ctgaacaagc ctgttgcgtt tccaaaccaa aaaaggccct tccagcctcg
     4021 acactatatt ccaaggcctc catcccctga gcaaaccacg cgtcaaacac accggtatcg
     4081 tcctctttgc cagccaagcc gaccacctcg gaaacgcgag caaaatatgc gagagtcgcc
     4141 tcgggatctc gatccgccac aagaaggccg aattttaata ttcgaatctt ccactcgacc
     4201 gtcgaaaccc caggcagaac ttctgtagcc ttggccaaaa aagctatcgc ctgctcagga
     4261 gaatgatccg caagcctcgt ccctagatca atgacacgtt gtttgacgtt cgcgtcattg
     4321 aactccagaa acagacttgg actcgttcga aaaaactcca aggttccaat ataatcaggc
     4381 ttcccaaggc tgttttgaac catgagcttc atgccaaacc caatccagga cctggccgat
     4441 tccaacggaa cagctctagc tatggaggga cattcacgga aaaactcgtt tcctaacaca
     4501 taattccatt cggacaggtc tatccccagt cgggcccatt cctgaagctc aggaatggcc
     4561 atctccccac ataattgggg aagcaccttg aaccattcgt atgcacattg tggagcggct
     4621 tcatttgacc catcggctag ttccagcaca tgggccaaca atgcatctcg attcgactcc
     4681 ccttccagaa ttccgagaat catgggactt tctttgaagt aacgcagacc gagagccccg
     4741 gaggcttgtg caaaggtaat ccctaaatcc agccatggaa tgacactggc tacgcatcct
     4801 tttcgattca actctcccag ggcccatacg gcttcaccct gtatttttga cgaaatctct
     4861 tttaattcaa tga
//
LOCUS       DAORAQ010000184         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2506_length_4858_cov_60.931709, whole genome shotgun sequence.
ACCESSION   DAORAQ010000184 DAORAQ010000000
VERSION     DAORAQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_4858_cov_60.931709"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKM72_12220"
     CDS             complement(<1..602)
                     /locus_tag="PKM72_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNP61600.1"
                     /translation="MNPSTQLDRKERELEAARRISQALFQHLSVEDVVEQGLKIALEV
                     VNCRAGSVLLANSETKELVFYHSIGDQPLKPGTAFPWTQGIAGSVFVTGDPVVIKDVK
                     EDQRHLEKIDQLSGFHTRDLIAIPLNRWEGHPIGVLEVMNKREDRLNQDDVAILMIIG
                     AFTALAIEQARLFQEAKLAEVARILGDIGHDVKNMLMPVM"
     gene            complement(767..3019)
                     /locus_tag="PKM72_12225"
     CDS             complement(767..3019)
                     /locus_tag="PKM72_12225"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNP61601.1"
                     /translation="MAKLIKAVLFGLLIGIVGVVFSVVPLSRSFEEDVGLGLLFQLRG
                     VRPPPTEVVVIRIDQDSSEHLNVPDNPDKWPRSLHARLTDGLVKAGAKVIVFDVNFLE
                     ARSPEEDVIFQKAIQRAGNVVLADAVHLKKVPMGVMAEAGAGPDSIVKISKPFALFKE
                     AAAGTGPFMLPRIPFRANQYWTFQKGRGDTPTFPILALQLFSSSVYEKFIDLLDQVHP
                     NLTGRLPNDFESIRTSKGLVELVRNIRKVFEGNPHLAEDMLKALGPVGSYRADVKNFQ
                     QLNALIKVYGSAQGRYLNFYGPPRTIPTISYYQILESESDATMGARNDLKDKAVFVGL
                     SEMFLVEGKDSLYTVFSQPNGVVIGGVEIAATVFANMLEDSFVQPLSLHAKISLLVSW
                     GLLVGMICRMVPTIMAAGAVLVLCFLYLGGTVYAFKTAFTWFPFLIPLLVQGPLAFGS
                     AVLWNYYETDRERKNIRKVFGDYLPNNVVDALSCDIGNLKGGPQVVNGTCLFIDTVNY
                     KTLLENLNPQEMNDMLGKYFEALFTPVRHHGGLIVDLKEDSILAIWKAPQDKLSLRKQ
                     ACFAALDIAKSVARFNREVAPYSLRIGIGLHCGQFAVGTVGAVDQSRLAGDVLNTPSR
                     VEGFNKYLGTTIAVTGDVVHGLEAAFLMRELGQFKLNRNEPSFVIYELISRFEEADPN
                     RMRASGIFAKGLGAFRIQDWNIARELFSQCVDLVDGDGPSEFYLQLCEQFIKHPPQEP
                     WDKVVTLEKK"
     gene            complement(3201..4529)
                     /locus_tag="PKM72_12230"
     CDS             complement(3201..4529)
                     /locus_tag="PKM72_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNP61602.1"
                     /translation="MEFECKSSSFQISVTERRKEPRRIEDRGLIQRNRELEAACRMTE
                     VLFQNFHTDEVVKKILTTALEVVGAESGSILLASQDSEELVFRHSIGARPVEIGTAIP
                     WDKGIAGAVFHSGQPIVIPDVKRDQRHYKAIDELTEHVTRDMVALPLKRWEGNPIGVL
                     EVLNKRESLLDEEDVAILTIVSALAASSIEQSRLYREAKLAVILGMLGDVSHDIKNML
                     MPVLSGAQLLEDELDEQFPHWIDVKMKGAETSYANCQELTRMIVNNARRIQRRVREIA
                     DAVKGVTSPPHFAPCLISTVVRAVFATLKTYAAEHAVLLETDGLDTLPVIEADEHRLF
                     TAFYNLISNAIPEIPGGGLISVSGEENNPGEGILITVSDNGQGMCPDVRDRLFHSEAI
                     TTKQEGTGLGTKIVKDVVDAHGGRIDVESELGVGTTFYLHLPVHPPAPRL"
BASE COUNT         1236 a         1357 c         1085 g         1180 t
ORIGIN      
        1 cacatgaccg gcatcagcat attttttacg tcatgcccaa tgtctcccag gattctggcc
       61 acttccgcta atttggcttc ctgaaataat cgcgcctgct cgatggccag tgcggtgaag
      121 gccccgataa tcatgaggat ggccacgtcg tcctgattta atctgtcctc ccgcttgttc
      181 atcacctcca ggacgcctat cggatgtcct tcccatcggt tcagaggaat ggcaatcagg
      241 tccctggtat ggaatccgct cagttgatcg attttctcaa ggtgacgctg gtcctccttc
      301 acatccttaa tcaccacagg gtctcccgtg acaaaaaccg atcctgcaat gccttgcgtc
      361 catggaaatg cggttccggg cttgagtggc tggtcaccaa ttgaatgata aaacaccaat
      421 tctttcgttt ccgaattggc caacaacacg gaaccggccc ggcagttcac cacctccaag
      481 gctatcttta atccctgctc caccacatct tccacgctga gatgctgaaa aagcgcttgg
      541 ctaattctcc gggcggcttc cagttcccgc tcctttcgat ccagttgggt cgagggattc
      601 acggctcgct ccccaatgat gtcaaagttg accagttctt caccatagcg gtaccacttt
      661 tggagaatta tcagaaaagc gtagcaaaat ttcaaaaaaa tagcttgtag ggaatttaag
      721 ggatcaccca ggaggaaggg gcaagcagga tcagacaaaa tcagggctat tttttctcta
      781 gtgtcaccac tttatcccat ggttcctgcg gtggatgttt gatgaactgt tcacagagtt
      841 ggagataaaa ttccgaaggt ccatctccat ctacaagatc aacacactga gagaacaatt
      901 ctcgggctat attccaatct tgtattctaa atgcaccaag gcccttagca aaaatcccac
      961 tcgctctcat tcgatttggg tcagcctcct cgaagcgact gatgagttcg tagatgacaa
     1021 acgacggctc atttctgttg agtttaaatt gtccaagctc tctcatgaga aaggctgctt
     1081 caagtccatg gacgacatcg cctgtcacgg cgattgtcgt tccaagatac ttattaaacc
     1141 cttccacccg ggagggagta ttgagcacat ctccagcgag acgggactga tcgaccgccc
     1201 ccaccgttcc gacggcaaac tgcccgcaat gcaatccgat cccgatgcga agactatacg
     1261 gtgccacttc ccggttaaac cgagcaacag atttcgcaat atccaatgcc gcgaaacacg
     1321 cttgtttcct caagctcagc ttatcctgtg gcgctttcca gatcgccagg atcgaatctt
     1381 ccttcagatc gacgatgagc ccaccatggt ggcggacggg cgtaaagagc gcttcaaagt
     1441 atttcccaag catatcgttc atttcctgag gattcagatt ttccaataat gtcttataat
     1501 tgacggtatc gataaacaga caggttccat tgacgacttg agggccgccc ttgagatttc
     1561 cgatgtcgca ggacagcgca tccaccacat tattgggcag atagtcgcca aaaaccttcc
     1621 gaatattctt tcgctcccta tcggtctcgt aatagttcca caagaccgca ctcccgaaag
     1681 ccagcggacc ctgcactaac aagggaatca ggaagggaaa ccacgtgaag gcggttttaa
     1741 atgcataaac ggttcctccc aggtacagga aacataagac aaggaccgct cctgccgcca
     1801 ttatcgtcgg gaccattcgg caaatcatcc caacaagtag cccccaactc acgagaagcg
     1861 atattttggc atgcagactc aggggttgga cgaaagagtc ctccagcata ttggcaaaaa
     1921 ccgtggccgc aatttcaacg cctccaatga cgacgccatt aggctgggaa aacaccgtat
     1981 acaaactatc ctttccttcc acaaggaaca tttcggaaag tcccacaaag acggctttgt
     2041 ctttcagatc atttcgagca cccatggtgg catcagactc ggattccagg atctgataat
     2101 aggaaatggt tgggatcgtc cgagggggtc catagaaatt gagataccga ccctgagcgc
     2161 ttccgtaaac cttgataagt gcgttgagtt gctggaagtt ctttacatca gctcgatagg
     2221 atcccacagg cccgagggct ttgagcatgt cttctgccaa atgtggattg ccctcaaaga
     2281 cctttctgat attccgtact aactccacca ggcctttgct ggttcgaatt gactcaaaat
     2341 catttggaag tcggccggtt aaattgggat gcacctgatc aagaagatca ataaactttt
     2401 catagaccga tgaagaaaac aattggagtg ccaggattgg aaaagttggc gtatcgcccc
     2461 ttcctttttg aaacgtccaa tactggttcg ccctaaaggg gattcttgga agcataaatg
     2521 gcccggttcc ggcggccgct tccttaaaga gcgcaaatgg cttcgagatt ttcacgatgc
     2581 tatccgggcc agcaccggct tctgccatca cacccatggg aacttttttc aaatgtaccg
     2641 catctgccag caccacatta ccggctcgct gaatagcttt ttggaaaatt acatcctcct
     2701 ccggagatcg ggcttccaga aaattgacgt cgaagacaat gactttggct ccggctttta
     2761 ccagcccgtc cgttagccga gcatgaagag agcgaggcca tttatcagga ttatcaggga
     2821 cgttgagatg ctctgaagaa tcctgatcaa tacgaatgac caccacctca gtcggtggcg
     2881 gcctgacgcc tcgcaattgg aacaagagac ctaatccaac atcttcttca aaactacgag
     2941 agagagggac aacactgaaa acaactccca cgataccaat gagcagacca aacaaaacgg
     3001 ccttaatgag tttggccacg aatgcctctc ctctgattct gggaatctca ttcctgaaga
     3061 taccaccaga accgtcagta cccactgctc catgtgggca tttccgaacg acctatccca
     3121 tcagcttcat actcgtcgtc cactgagaag cacacaccaa tcagccatcc tcacaataag
     3181 accagccgtc gggacaattt tcacaatctc ggtgcaggag ggtgcacagg aagatgcaga
     3241 taaaacgttg tgcccacacc cagctcactt tctacatcaa tgcgtcctcc atgggcatcc
     3301 accacatctt tgacaatttt ggtccccagc cccgtccctt cctgcttggt ggtgatggcc
     3361 tcggagtgaa aaaggcgatc ccgcacgtcg ggacacatgc cctgaccatt gtccgaaaca
     3421 gtgatgagaa tcccctctcc tggattgttc tcctctccag aaaccgagat caggcctcct
     3481 ccgggaatct ccggaatggc attgctgatg agattgtaaa acgcggtaaa aagacggtgc
     3541 tcatccgctt caatgacagg gagggtgtcc aggccgtcgg tttcaagtag gaccgcgtgc
     3601 tccgccgcat aggttttaag ggttgcaaac acggccctca ccacagtgga aatcaggcag
     3661 ggggcgaagt ggggtggact cgtgacacct ttgaccgcat cggcaatttc ccgcacgcgc
     3721 cgttgtatcc gcctggcatt gttcacaatc atcctggtga gttcctgaca attggcataa
     3781 ctggtctcag cccctttcat cttcacgtcg atccaatgag gaaattgctc atccaattca
     3841 tcctccaata attgggcccc tgataacacc ggcatcaaca tattcttaat atcatgacta
     3901 acatcaccca acattcccaa gatgacggcg agcttcgctt cccggtacag acgggattgc
     3961 tcaatcgagc tagcggcaag ggctgacaca attgtcagga tagccacgtc ttcttcatca
     4021 agaagcgatt cgcgtttgtt caggacttcc aacacaccga tagggtttcc ttcccaccgt
     4081 tttaaaggca gagcgaccat atcccgtgtt acgtgctcgg ttaactcatc aatggcttta
     4141 taatgccgtt gatcccgttt gacatccggg atcacgatcg gctgtccgga atgaaacacg
     4201 gccccggcaa tccccttgtc ccatggaatc gccgttccga tttctaccgg tctggctcca
     4261 atggaatgac ggaacacgag ctcctcggaa tcctgactcg cgagaagaat ggatccgctc
     4321 tccgctccaa ctacctccag cgcagtagta agtatttttt taaccacctc atcggtatgg
     4381 aaattctgga aaagcacttc cgtcatccgg caggccgctt ccaactcccg gttgcgctgt
     4441 atcaatcctc gatcttctat ccggcgaggc tcttttcggc gttccgtaac agaaatctga
     4501 aacgaggagg atttgcattc aaattccaca gatatatctc ctatgaaggt tcgcgagcta
     4561 tcccatacag acttacccac gtgattcata aaatttcata cagccggtca atgcatggtt
     4621 tgtttccacg ttaaatacca ttacctctca ccctcctggc tgcctttgaa tttcgaacaa
     4681 ctaacattga ggtatcggcc attcctgttg gctcccttct tcagtatcgg ccatcaccgg
     4741 ataggacgac atgtggtacg ccagcgcatt tacctttgct gaaacgacca atttccaccc
     4801 ggtgagagaa aacgttcggt catcccttgg cgaccacacc cttcccatca aggatggt
//
LOCUS       DAORAQ010000185         4853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2511_length_4853_cov_59.846603, whole genome shotgun sequence.
ACCESSION   DAORAQ010000185 DAORAQ010000000
VERSION     DAORAQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2511_length_4853_cov_59.846603"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..443
                     /locus_tag="PKM72_12235"
     CDS             57..443
                     /locus_tag="PKM72_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61603.1"
                     /translation="MSMSTNMTYLTDVAMITCVVQSGDADVIVKAARDAGATGVLVHQ
                     GRGIGIRERLGILGVAVDASKDIISVLVSFEQTDLVASHIFKAGQLDTPAKGFLYITP
                     LEKVAMYIPEAVREQLRQKGSASERS"
     gene            440..805
                     /locus_tag="PKM72_12240"
     CDS             440..805
                     /locus_tag="PKM72_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61604.1"
                     /translation="MSAGQAADHLMFPEPSQIITCVLPDDGTDRVLLRRLREEKGIIR
                     ADSVSCRGIAALREAFTKNGRLPESTLVRMVNIVVSVTEAEALFEYIYEAAHVGHPGG
                     GMLILSKPVMTTPYSLPID"
     gene            complement(1138..2826)
                     /locus_tag="PKM72_12245"
     CDS             complement(1138..2826)
                     /locus_tag="PKM72_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNP61605.1"
                     /translation="MGLQSKMADLGRLHEIIRAFSVAGFGDIFKKMGLEAAAEQTGKI
                     MGWQYAEDIAHLERPQRIRKLFEVLGPTFVKLGQILATRPDLFGPEWISEFEKLQSQA
                     PQLDFEELRPQIEEDLGAPLEDLFAEVDTKPLAAASMAQVHRATLKDGTKVVLKIQRP
                     GIREKIEADMRLLTHVASLAENNVSELATYHPQKVVQQFVKSLQNELNFMTEGQNAEQ
                     VSANFEDNDRVVIPKIYWEWTKERISVQEFVEGIPGVNIQAIDQARMDRKRLARTGAN
                     AVLKMIMVDGLFHADPHPGNFFILPGERIAFIDFGMVGRVSEIRRQQIMRLLQALLQN
                     DAEGLCSILLQWSEREGEDPGDLLVPVDEFLGKYSGRSMEHLDLSVMVGDLLALIRNN
                     KLTMPPDQAMLLKVFVSLEGAFKKLDPGFDMMAAIQPTLQEAVLNQLSPKALGKRGLK
                     VLIQYLELFADIPKEIRRGIYTVKTGNLKFCIELTQLDELKHVLIRAVSRLAVAAITS
                     ALVIGTSIVMALSKGPIIGGINIYQIFGVGAIIGGVFILLAMMRDKTWKKNLKE"
     gene            3250..4332
                     /locus_tag="PKM72_12250"
     CDS             3250..4332
                     /locus_tag="PKM72_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61606.1"
                     /translation="MTMWVSSSVNLPLVFPSVKKSFLKSGFTTMVEIVVSSCLILVAF
                     TGPAMAVPTAEELLKALPLSDGERRDVLKGEIVRFTTEEGSDRELALGAVLLVPKVPE
                     NLVPLWREAVVFKLVGAVTAFGEISDKANEGAFAGVKLQPNGEKEADRYLEAEPGEKL
                     NLNMKEISAFQALKAKGVGQAEVEALVRKQLLARYQAYRENGLSGIPPYARGKDEMFQ
                     LREDLLLATNQAKLLAKYFPSFHQALLKYPANQPKQLETRFNWANVDVFSRPTLILSH
                     RMLFQEGDAYVVVDRHFYASHEYNGLQSIGAALPAKEGSLLVSLYRISTDGVAGLGSS
                     AKHPVARGLMGPYFEEIFEGIREKAK"
     gene            4407..4808
                     /locus_tag="PKM72_12255"
     CDS             4407..4808
                     /locus_tag="PKM72_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61607.1"
                     /translation="MCWMRWVGMSWIVGGELAIPMNMQPAWAGKAAEIDRDVGAALEK
                     FYAHRPVAKDISTIAQRIPILPDVYKAEFMHAGQFGDEALCKQGKIVGYYRTVAASYG
                     LQAGIHSIHPSIPSIHPFLLLCVILHPGESP"
BASE COUNT         1168 a         1153 c         1235 g         1297 t
ORIGIN      
        1 cgtgcggtta aagaaacgag gggcggctca caactccagg aggtgcaatc catcgtatga
       61 gtatgagtac gaacatgacg tatctcaccg acgtcgccat gattacctgt gtggtgcaat
      121 caggtgatgc cgatgtgatt gtgaaagctg cccgagatgc cggggcaacg ggagtattgg
      181 tccaccaggg taggggaata gggattcgcg aacgtttggg aatcttggga gtggcggtgg
      241 atgcctcaaa ggatatcatc agcgtcctgg tctctttcga acaaaccgat cttgtggcca
      301 gccacatttt taaagccggc cagttggata ctccagcaaa agggttctta tacatcacac
      361 ccttagaaaa ggttgccatg tacatcccag aggctgtgag agaacaattg cggcaaaaag
      421 ggtcagcctc ggaacgatca tgagcgccgg acaagccgcc gatcatttaa tgttccctga
      481 gcccagtcag atcattacat gtgttctgcc ggatgatggc acggacagag tattactcag
      541 gaggttgcgt gaggaaaagg gcatcattcg tgcggacagc gtttcctgtc gaggcattgc
      601 tgcccttcgg gaagccttca cgaaaaatgg tcgtttgccc gaatcaactt tggtgaggat
      661 ggtaaatatc gttgtgtcgg tgacagaagc cgaagcgttg ttcgagtaca tctatgaagc
      721 agcccatgtc ggccatcccg ggggtgggat gttgattttg agcaagcctg tcatgaccac
      781 tccttactct cttcccatag attgatgtca attacacaac aaacatcatc tacccaccat
      841 ggaaaacaaa acctgccttt ttttcgcttt tcgtaacttc aggagaagcc ttttgatcta
      901 gccggatcat gtggggaagc acagtatttt tattccacat cctttcctcg tgagtgcagt
      961 tggccttgaa gtttcgtggg atgagccgtc gatgtgaatg aaagtggttc ggaaactgtg
     1021 ctgctgttgt cttatcatgc tcgttagttg ccagcgtcgc agccagccaa gctttaagtt
     1081 ttcaaaccta tttcattaaa acagcccggg atagccctca gcttgcggag gggagcttca
     1141 ctccttgaga ttttttttcc aggttttgtc ccgcatcatg gcaaggagga taaagactcc
     1201 accgatgatg gctccaaccc caaaaatttg gtagatattg atccccccga tgatcgggcc
     1261 cttggaaaga gccataacaa tcgaggttcc gatgaccaga gctgaggtta tcgcagccac
     1321 ggccagtcga ctgacagctc gtatgagcac gtgtttgagt tcatcaagtt gagtcagttc
     1381 tatacaaaat ttcagattgc cggttttgac ggtatagatt ccccggcgta tctccttggg
     1441 tatgtcagcg aataactcta aatattgtat gagaactttg agaccgcgtt tcccgagggc
     1501 tttaggagac aactggttga gtacagcctc ttgaagggtg ggctgtattg cggccatcat
     1561 gtcaaaacca ggatcaagct tcttaaacgc cccttccagt gacacaaaaa ccttgaggag
     1621 catagcctga tcgggaggca tggtaagctt gttattccgg atcaatgcta acaggtctcc
     1681 caccatgacc gacaaatcca aatgttccat ggatctcccg gaatacttgc ccagaaattc
     1741 atcaacaggt accaagaggt cgcccgggtc ttctccttcc cgctcagacc attgtaaaag
     1801 gattgagcag aggccttcgg cgtcattttg caacaatgcc tgcaacagtc tcatgatttg
     1861 ttgccgtctg atttcagaga cgcgtccaac catgccaaag tcaatgaagg caatacgttc
     1921 acccggcaaa atgaaaaaat ttcctgggtg cggatccgca tggaataatc catcaaccat
     1981 aatcattttg agcacggcat tggcccccgt ccgggcaagg cgtttgcggt ccatccttgc
     2041 ttgatcgatg gcctgaatat tcacgccggg aatcccctcc acaaattcct gcacgctaat
     2101 gcgttccttt gtccattccc agtaaatttt gggaatcacg acccggtcat tgtcctcaaa
     2161 gttggccgag acttgctccg cattctgtcc ttccgtcatg aagttcagtt cattctgcaa
     2221 tgatttcacg aattgctgaa caactttttg aggatgatag gtggcgagtt ccgaaacgtt
     2281 attttctgct aacgaggcca cgtgggttaa tagacgcata tcggcttcga tcttctcccg
     2341 gattccaggg cgttgaattt tgagtacgac tttcgttccg tctttcaacg tggcccggtg
     2401 aacctgggcc atggaagcgg cagccagtgg tttggtgtcc acctctgcga agaggtcttc
     2461 aagtggcgct cccaaatctt cctcgatctg agggcgaagc tcctcgaagt ccagttgtgg
     2521 tgcctgcgat tgaagttttt caaattcact gatccactca ggtccgaaca ggtcagggcg
     2581 agtggccagg atttgaccaa gtttgacaaa tgttggcccg agtacttcaa agagtttccg
     2641 tatacgctga ggacgttcaa ggtgggcaat gtcctctgca tattgccacc ccataatctt
     2701 gcctgtttgt tccgctgcgg cttcaagccc cattttttta aatatatccc cgaatcctgc
     2761 gacactgaat gctctgatga tttcgtgcaa ccgacccagg tcggccattt tggattggag
     2821 ccccatcctc aattcctcct taccatgtcc atatttttgg tgctattgta cttccctcca
     2881 gggttcagcc ggaccccaaa gacaagtttt gaaaaatccc cattcttcag aagaaagggt
     2941 ggaatgcttt tttaatgtct gtttacgact actttgttgt tccaatctgg aatcttccag
     3001 gaaaatttgc tgtaatattg acacaattag ttggagaatg aaaaggaaag gcaaaagatc
     3061 ggatttgatg gtcgggttct ttaagccggg ataaagaaac tattcattgt gaagcccttt
     3121 agactgaggg tgtcctcgtt cttcacttta atggaatagg tgtttgctct ctggcaatct
     3181 tgtccctaac cttcaaaatc caatgcctgg tgtatttcta tcaatggttt ttttattagg
     3241 aaggggaaaa tgactatgtg ggtttcatct agcgtgaatc ttccattggt atttccttcg
     3301 gtaaaaaaaa gttttttgaa atccgggttc acgaccatgg ttgagatagt ggtaagttcc
     3361 tgtctcatcc tggtggcctt caccggtcct gcgatggccg ttcctacagc cgaggaattg
     3421 ctgaaggcgt tgcctctttc cgacggtgaa cggagagatg tcctcaaggg agagattgtc
     3481 cgttttacga ccgaagaagg ctcggatcgg gaactggcct tgggagccgt attgctggtg
     3541 ccgaaagtcc ctgaaaattt agttccactc tggagggagg ccgtcgtgtt caagcttgtc
     3601 ggtgcggtga cggcgtttgg cgagatttct gataaggcaa atgaaggtgc ctttgcagga
     3661 gtgaaattgc aaccaaacgg tgaaaaagaa gccgaccgct acctggaagc tgaaccgggt
     3721 gaaaaattga atctgaacat gaaagaaatc tccgcttttc aggcactcaa ggccaaaggg
     3781 gtagggcagg ctgaagtcga ggcactggtg cggaagcaat tgctggcccg gtaccaggcc
     3841 tatcgcgaaa acggtctgtc aggcattcct ccatacgcgc ggggaaaaga tgaaatgttt
     3901 cagttacgtg aggaccttct tttggctacc aatcaggcca aactcctagc caaatacttt
     3961 ccctctttcc atcaagccct acttaagtat cccgccaacc agcccaagca gctcgaaacc
     4021 cggtttaact gggccaatgt tgacgtattt agccgtccga cattgatctt gagccatcgc
     4081 atgttatttc aagagggtga cgcgtatgtg gtggttgacc gccatttcta tgccagccac
     4141 gagtataacg gcttgcagtc catcggggcc gcacttcccg cgaaagaggg ctcgctgtta
     4201 gtttctcttt atcgtatctc caccgatggt gtggcaggtt taggatcctc ggcgaagcat
     4261 ccagtcgccc gtgggttgat ggggccctat ttcgaagaaa tctttgaggg gattcgtgaa
     4321 aaagcgaaat aatgactccg cacgagttgt taggaaattt gttccacctg catttttcgg
     4381 gttgctttca cattaagggg ttcaaaatgt gttggatgcg atgggtggga atgtcctgga
     4441 ttgttggggg tgaattggct atccccatga atatgcaacc ggcgtgggcc ggtaaggctg
     4501 ctgaaattga ccgtgatgtt ggtgccgcct tggaaaaatt ttatgcccac cgtcccgtcg
     4561 caaaggatat ttctacgatc gctcagagaa ttcccatcct tcctgatgtc tataaggcag
     4621 agttcatgca cgccggacaa ttcggggatg aggcgctttg taaacaaggc aaaatagttg
     4681 ggtattacag gactgttgcg gcttcttatg gattgcaggc aggcatccat tccatccatc
     4741 catccatccc atccatccat ccattccttc tgctatgcgt tattcttcat ccaggagaaa
     4801 gcccttgagt atctcaacaa gagtaaaggt tgggagatcg gcgtaggtcc gag
//
LOCUS       DAORAQ010000186         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2513_length_4851_cov_72.644704, whole genome shotgun sequence.
ACCESSION   DAORAQ010000186 DAORAQ010000000
VERSION     DAORAQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2513_length_4851_cov_72.644704"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PKM72_12260"
     CDS             complement(<1..1351)
                     /locus_tag="PKM72_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNP61608.1"
                     /translation="MTEDHGELSHEKNSSEESPLTRRQLLRAAVGVGGGLLASGLAAC
                     AEGPQRLSDAGSNGMGLATSTPVIDEAGLTANSSGIAHFTQPEVRRSKHGLLNTQLRV
                     AFANNTLNGEPLPPNRSYEGGLTGPTFRIKAGDRMKVYLFNQLPPEHGECGPPNTENC
                     PNTTNLHTHGLHISPTGNSDNVLLEIPPGNDLQFQFDIPDHHHAGTFWYHAHRHGSTA
                     SQLREGMAGALIIEGDIDQVPEIKVARDQVFLFQQLRIPFDKPENQLPTTINGLLEPV
                     LTLQPGEVQRWRLIHAGIDELLTIELVNEEGVPQDLHVIAIDGITLGKIDTSKEVFLA
                     PGNRADVLIKAGPKGIYRLKKKMEEVGLDGNPESEQLLANIQVMGPRINMSLPSNREL
                     RPLAPFRPITDQELTDPPKQPELVFDIANGQFMIDGQEFDHTRVDRTMTLNGVQEWTL
                     "
     gene            1557..3155
                     /locus_tag="PKM72_12265"
     CDS             1557..3155
                     /locus_tag="PKM72_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNP61609.1"
                     /translation="MPAKYDAIVIGSGHNGLTCACYLAKAGLKVLVLEQYHSIGGMTN
                     TEELTLPGFQSDTHAFCVQVGSFSPALHELQLARYGFELIYSDPCFSHVFPNGRSISV
                     RRNVDETCQSIAQFSKMDSATWRALFERFLTVKDQIAASINTPPPSFADQASTLQNLP
                     GGLDEYRFQLQTFRSWCNEMFEVDETKALFGSWDLHVGTSPDDVGGASMAWLFTMVIQ
                     HFGNNVVKGGMRNLPLALAGFLEAHGGEIKTEARVNDIIVENGKAIAVRLAGGEEVEV
                     GTLVASNVDPQQLVLHLLGEDRVGTNLAKKMRQYELGESVMVVYLALDSPVEYKAGPL
                     AGQSVYVHPTPASLDYFSRLFHEVRGGRLPAEPFCLVCNDSAGDSTRVPSGRGLMKLV
                     VQPVPYALKDDATGKIRGRTWNEAKEPFADYVIDRLTRDYIPDLRNKILKRVVHSPKD
                     LEELMPSAPQGTITHGAFVPYQMGSMRPIPELGHYRSPVRNVYLCGSGSHPGAGVTMA
                     PGRNAAQIIYQDLNLDFAKTLATH"
     gene            complement(3542..4537)
                     /locus_tag="PKM72_12270"
     CDS             complement(3542..4537)
                     /locus_tag="PKM72_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNP61610.1"
                     /translation="MNTSRWVVMAGTLFVVCAGGWYGMIWMPGSSYQGALPLATDETR
                     DLEQKLHTHVHMLSIQIGERNLFHYDKLLLAAEYIRDEFKAAGYDVLGQKFDVSGNIC
                     ENIVAEVPGTDRREDILVIGAHYDSVKGSPGANDNGTGIAAMLALARQFAQSPVSRTL
                     RFVAFSNEEPPFFQTTSMGSRVYAQRSRQQNERIILMLSLETIGYYSEASNSQRYPPP
                     LSLIYPSKGNFIGFVSNIGNRDWVQKAVGFFRQHTQFPSEGAALPEWLPGVGWSDHWA
                     FWQEGFPALMVTDTALFRDPNYHTSNDTPDHIHFGHLALVVTGLQHVIRDMANQP"
BASE COUNT         1102 a         1199 c         1295 g         1255 t
ORIGIN      
        1 ataacgtcca ctcctggacc ccattgagtg tcatggtccg atccacacga gtatggtcga
       61 actcttgccc gtcaatcatg aactgaccgt tggcgatgtc aaataccaat tccggctgct
      121 taggcgggtc agtgagttct tggtccgtaa tcgggcgaaa tggagccaaa ggccgaagct
      181 cgcggttgct ggggagactc atgttgattc gtgggcccat tacctgaata ttcgccaaga
      241 gttgctcgga ttctggattt ccgtccagcc cgacttcttc catctttttt tttaatctgt
      301 atatgccttt gggccccgct ttgatcaaca cgtcggcgcg attgccgggg gccagaaaaa
      361 cctccttact cgtatcgatc tttcctaggg taatcccatc aatggcaatg acatggagat
      421 cttgaggaac gccttcttca ttcacaagtt cgattgtgag caattcatcg atgccggcat
      481 ggatcaagcg ccaacgttga acctcgcccg gctgaagggt tagcaccggt tccaataatc
      541 cattgattgt ggttggcaac tggttttcgg gcttgtcgaa cggaatcctg agctgctgaa
      601 aaagaaacac ttgatcgcga gccaccttga tttccggcac ttgatcgata tcgccttcaa
      661 taatcaacgc gccggccatg ccttctcgca attgagatgc cgtcgatcca tgtcggtgcg
      721 cgtgatacca gaaggttccg gcatgatgat ggtcaggaat atcaaactgg aattgaagat
      781 cgtttccggg aggaatttca agcaggacat tgtcactatt gcccgtcggt gagatatgaa
      841 gcccatgcgt gtgtaaattg gtcgtgttcg ggcaattctc ggtgttgggt gggccacatt
      901 ctccgtgttc tggagggagt tggttgaaca agtagacttt catgcgatct ccggctttga
      961 ttcgaaaagt cggaccagtg agcccaccct cataggatcg attcggtggg agaggttctc
     1021 cgttgagtgt gttgttggca aaggccacac gcaattgggt attaaggagc ccgtgcttcg
     1081 atcgacgcac ttccggctgt gtaaagtgtg caatgcctga cgaatttgcg gtgagccctg
     1141 cttcatctat cacaggggtt gaagtggcta gccccatgcc gttcgatcct gcatcggaca
     1201 gtctctgagg cccttcagca catgcagcaa gaccggatgc cagaagtcca ccacccacac
     1261 cgactgctgc ccgtaacaac tgacgtcgag ttaagggaga ttcctctgag gagttttttt
     1321 cgtgtgataa ttccccatga tcctcggtca ttgcgttcct ccttttcaat aggtgatcag
     1381 gatttcacat acatgatatc tagaaaaaaa cggaacagcg gtacttcttc tatggtccat
     1441 tgaagacttc gaggtgagtc tggtggatcc gcggggcatc gctttgtata ctatcgcctt
     1501 gtggattttc aataaagaga tattggcaaa tatctgagag tttgtggaga aaaattatgc
     1561 cagccaaata tgacgcgatt gtgattggaa gtggtcataa cggactgaca tgcgcctgct
     1621 atcttgcgaa agccggactc aaggttcttg tgctggaaca gtatcattca atcggtggca
     1681 tgaccaacac ggaagagctc accctacccg gttttcagtc agacacacat gcgttctgcg
     1741 ttcaggttgg gagcttttcc ccggcgctcc atgagctgca gttagcccga tacggtttcg
     1801 agctgatcta ttccgatcct tgcttttccc acgtcttccc gaacggtcgc tcgatctctg
     1861 tccgccggaa tgtcgatgag acgtgtcaga gtatcgctca attttcaaaa atggactctg
     1921 ccacgtggcg tgcgctattc gaacgatttc tcacggtgaa ggaccagatt gccgcctcaa
     1981 tcaatacccc accaccttct tttgcggatc aagcatccac attgcagaat ttaccgggtg
     2041 gcctggacga ataccgcttt caactgcaaa ccttccggtc atggtgtaac gagatgttcg
     2101 aggtggatga aacgaaggct ctattcgggt cctgggattt gcatgtgggg acttctccgg
     2161 atgatgtggg cggcgcgagt atggcatggc tcttcacgat ggtgattcag cactttggaa
     2221 acaacgtggt gaaagggggt atgcgtaatt tacccctggc acttgctggt ttcctggaag
     2281 cacacggggg agaaataaaa acggaagccc gagtcaatga catcatcgtg gagaatggga
     2341 aggcgattgc cgtacggctt gctggagggg aagaggtgga ggttggcaca ctggtcgctt
     2401 caaatgttga tcctcaacaa ctcgttctcc acctgctcgg cgaagaccgg gtagggacga
     2461 accttgccaa gaagatgcgg cagtatgagt tgggggaatc ggttatggtc gtctaccttg
     2521 cgcttgatag ccctgtcgag tacaaagcgg gaccgctggc cgggcaatct gtctatgtcc
     2581 atcccacccc ggcgtccctg gattactttt ctcgtctttt ccatgaagtt cgtggtggtc
     2641 gtttaccagc agagccattt tgtcttgtgt gtaacgactc tgccggagac tccacgcgcg
     2701 tgccttccgg caggggttta atgaaactcg tcgtgcaacc tgttccatat gccctgaagg
     2761 acgatgccac cggcaagatt agggggagaa cttggaacga agctaaagaa ccctttgcgg
     2821 attatgtcat cgataggtta acgagggatt atatccccga tcttcgaaac aaaattttga
     2881 aaagagttgt ccatagtccc aaggatctcg aggagttgat gccgagtgcc cctcaaggga
     2941 cgatcaccca tggcgccttc gtgccctatc agatgggctc catgcgtccg atacctgagt
     3001 tgggacatta tcggtctccg gttcgcaacg tttatttgtg cgggtccggc agccatccgg
     3061 gggcgggcgt gaccatggcg ccgggacgaa atgccgcaca aattatctac caggatctca
     3121 atctcgattt tgcaaaaacc ctcgccacgc attaattcct agactctcat ccgttctcgg
     3181 gctttctacc gagcaagagg ccattggctc cgggagaacg aattggaata cggcgcacgt
     3241 ctgtgactcc cacttccttc aaccaggccg tgtactcact ccaggtatag ttcctgcttt
     3301 tcgtattgat taacatgcac agtgacatca tggcagccgc gagtggccct gtcttatcgt
     3361 cgtccatcat cagttcgctg acaatgatag aaccacctgg ctcaagagcg tctaaatatt
     3421 tccgcagagc aagtcgaaca gtgataagga attttgtgta gtggtacaat ttgaaaaggt
     3481 gactgacgta agggtgaaac gagaggataa atgagaggtc tgtttctccc cataccgttt
     3541 gtcatggttg gttggccata tccctgatga catgttgcag tcccgtgacc acaagtgcga
     3601 ggtggccgaa gtgaatgtga tcaggtgtat cgttcgaggt gtggtaattc ggatctcgaa
     3661 atagagcggt atccgtgacc ataagggcag ggaatccttc ctgccagaat gcccaatgat
     3721 ccgaccagcc cactccgggc aaccattcag gtaacgcggc cccttctgag ggaaattggg
     3781 tgtgctgccg aaagaatcct accgccttct gcacccaatc cctgtttcca atgttactca
     3841 caaaaccgat gaaatttccc tttgatgggt aaatgagact gagcgggggt gggtatcttt
     3901 ggctgttcga tgcctcagaa tagtagccaa tcgtttcaag gctgagcatg aggataatgc
     3961 gttcattctg ctggcgactg cgttgggcat atacgcggct ccccatactt gtggtttgaa
     4021 agaagggggg ttcctcgttg gagaaggcta caaacctgag ggtgcgagaa acaggggatt
     4081 gggcaaactg ccgggccagg gccaacatcg ctgcgattcc ggtcccgtta tcattcgcgc
     4141 cgggacttcc cttgaccgaa tcataatgcg cgccaatgac caggatgtct tcacgtcgat
     4201 ccgtgccggg tacttccgct acgatgtttt cacagatgtt tccggaaacg tcgaattttt
     4261 ggccgaggac atcatatccg gctgctttga attcatcgcg aatatattcg gctgcaagca
     4321 ggagtttgtc gtagtgaaag agattgcgtt ctcctatttg tatgctcagc atgtggacat
     4381 gggtgtggag tttttgctcc aggtcacgtg tttcgtcggt cgctaatggc aaggctcctt
     4441 gataactact ccccggcatc caaatcattc cgtaccatcc tcctgcacac acaacgaaca
     4501 acgttcccgc catgacgacc caccgtgagg tgttcatctt tcttctccga cctttcagaa
     4561 tcagggtcga tccattcctg cacgtgaatt cgcccacaga ggtgcggtgt tatttggcat
     4621 tcttcagtag gataggctta tgtgatgaaa tagcaatggg atcgccatta ggcacagatg
     4681 cttcgctttc ggctggggat gacatgttgg aaatcatcca tcgttattta agattccatt
     4741 acttttcggg caaccaacca gccatgacac agcgttgtgc gaagagcggc acgaagcttt
     4801 gggtgtcgag ggaaatcgta ttcatgaatt ggtctgccaa tgtttgatcg g
//
LOCUS       DAORAQ010000187         4845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2514_length_4845_cov_71.054071, whole genome shotgun sequence.
ACCESSION   DAORAQ010000187 DAORAQ010000000
VERSION     DAORAQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_4845_cov_71.054071"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..426)
                     /locus_tag="PKM72_12275"
     CDS             complement(76..426)
                     /locus_tag="PKM72_12275"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNP61611.1"
                     /translation="MEINDALLAFAALSQDTRLRVFRLLVEYGPDGAPAGTLGEALSI
                     PHNTLSFHLSHMSNAGLVLSQRKGRSIIYSANFEFFTDLIRYMVKDCCREDMASIRDN
                     KKKKCSIIELSNCC"
     gene            complement(662..1018)
                     /locus_tag="PKM72_12280"
     CDS             complement(662..1018)
                     /locus_tag="PKM72_12280"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNP61612.1"
                     /translation="MGTHKSNNEEKAGKVLTFQLPLTKDEVSTKNSKKGNSKAVSRLA
                     ERRKGVETRQVQSGFPHFILEEALQGRIAKRAYELFEQRGRQHGYDREDWYQAENDML
                     TQKDVEYKEEPGCKNS"
     gene            complement(1287..1784)
                     /locus_tag="PKM72_12285"
     CDS             complement(1287..1784)
                     /locus_tag="PKM72_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61613.1"
                     /translation="MLARILVVDDEADSACSIAEAMEGWGFEAVTTSNGREGLHVLHT
                     MPIDGIFLDLEMPVMNGLTMLDELRWEGSEVPVIVMSNEGDYLKLQKFLREGVQDYLV
                     KPINHLLLMQKTFRHFPWRESVGKRQAFVKDTIHEAKHPLAEGALMIQGCCALSSRKP
                     DWKVA"
     gene            complement(2024..2200)
                     /locus_tag="PKM72_12290"
     CDS             complement(2024..2200)
                     /locus_tag="PKM72_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61614.1"
                     /translation="MMNRPETRRPPLSAPTEEEAVTDRTQILGDYSRPNSANGSMDKV
                     IKRFLIKRQEKVGT"
     gene            complement(2887..4611)
                     /locus_tag="PKM72_12295"
     CDS             complement(2887..4611)
                     /locus_tag="PKM72_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP61615.1"
                     /translation="MDEYERDLLYAKQVEQLYGLAPVGIIASLVNGSILTFIQWNVIA
                     HGILLTWFSCLIVLNGAWSLLWYQFRHGSRNPQDSHRWGRRFLGGTFASGLLWGTTGA
                     FLFPENSITHQIFLAFVLGGMIAGATAVYAALEGAFLAYTLPTITPLLVQFFILDDEH
                     HMAMGAMSLLFVTMMFVTLRRNHTVTIASMTLSLALGRSNQSLQSEVLERKQAEVALR
                     ESQEQLHSVVQSTDEGIISLNSQGKVMLWNAGAESLFGFSMKEMYGQTLERIIPERFR
                     LAHHHGIARASRAGGKTVEGEMFELKGLRRDGSEFPLELSLGYWHKHGEAFFTGIVRD
                     ITARKEAEQALHRRERELEHSQEELRALGAQLISAQEDERRRLSRELHDDMNQRLAVV
                     ALEIQSIQNTLPESNAMQKTLQHLNDQVSSLSDSVRHLAYQLHPSILDDLGLVVALQS
                     SIREFSQWENISVTFRPRDVPHALPQDIASCVYRVTQECLRNVAKHADATRVLVEVMG
                     LEAGLQLIITDNGKGFIPEAVRPGSQGLGLIGMKERIRVVQGTFKVKASKGKGTMITA
                     WIPLSSET"
BASE COUNT         1288 a         1271 c         1083 g         1203 t
ORIGIN      
        1 ccgatggctt cttccaactg ttcgactccg atatgaatat gaagacgttt catgggtgtg
       61 tctccttgtg agtggttagc aacaattcga gagttcgata atgctgcact tctttttttt
      121 attgtcccgg atactggcca tatcctcgcg gcagcagtct ttgaccatat agcgaatcag
      181 gtcggtaaaa aattcaaagt tggcgctata gatgattgac cggccttttc gctgagaaag
      241 gaccagaccc gcgttgctca tgtgggagag gtgaaaggat agggtgttat gcggaatgga
      301 cagagcctca cctaatgttc cggcaggggc cccgtccggg ccatattcca ccagcagacg
      361 aaaaacacgt agccgggtat cttgtgagag ggccgcaaaa gccagcaatg cgtcattaat
      421 ttccatatgt ctagaataat agacatataa aaaaaaagca agtaggtgaa atcccaaatt
      481 tcttcagtca aatcggaagg caagagaaaa aacgagtcga agactacgtg aaacgggaca
      541 gaggagtggg aagattcgtg attaaaacag gtgccttatc gaggccaatg gatccattca
      601 ggccggcacc tccatcccat attggcaaga atgagaatgc ttgggaagtc atgacactaa
      661 tttatgaatt tttgcaacca ggctcctcct tatactccac atctttttgg gttaacatat
      721 cgttttccgc ctgataccag tcctctctat catagccatg ttgtcggcct cgttgttcaa
      781 acaattcata ggcccgcttc gcgattcgcc cctgtaatgc ttcttccaaa atgaaatggg
      841 ggaaacctga ttgaacctgc ctggtctcta cacccttcct acgctcagcc agtcgagaca
      901 cggcttttga attccctttt ttggagttct tcgtggagac ctcatccttg gtaaggggaa
      961 gttgaaatgt taaaactttt ccggcctttt cttcattatt tgatttatga gttcccatag
     1021 tcttcctcct gttggtctgc tgctcacgag tttgtgataa atttcctttt cactctcaat
     1081 caagcttgct taatcctgtg cataccgatc cccattcact cgctttgttg tactacccac
     1141 gagccttcct ttcccggtga cgcgggaata atgtcaggga tttttggtcc ggacatctcc
     1201 acacaatgaa aaggagacaa tgcatcggca ccatgggaga aaagaaaagg tgtgagaatc
     1261 cgacacagac caacccctct cactgctcag gctactttcc aatcaggttt cctcgaagat
     1321 aacgcacaac aaccttggat catgagcgct ccttccgcca gaggatgttt agcctcatga
     1381 atggtatctt tgacgaaagc ctgtcttttc cccacgcttt ctcgccaagg gaaatgtcga
     1441 aatgttttct gcatcaagag aagatgattg atcggtttca ccagataatc ctgaacacct
     1501 tctctcagaa atttctgaag tttcagataa tcgccctcat tcgacatgac aatgacggga
     1561 acctcgctac cctcccatcg cagttcatcc agcatggtca atccattcat caccggcatt
     1621 tccaaatcaa gaaaaatacc atcaataggc atagtgtgaa gcacgtggag accttcccgc
     1681 ccatttgaag tcgtcaccgc ctcgaatccc cacccctcca tcgcctcggc tatggagcag
     1741 gcgctgtctg cctcatcatc aacaaccaag atcctggcca acatcgtttc ttccatattc
     1801 aaaactcagc tcggggtgtt gctctcaggc gcggaaccca ctgtagtcgc aaaactcgct
     1861 gccacaaacc gtctgcccca taatcaacac aatcggtttg gccttgggac tcaaggatag
     1921 caagggcaaa tcggatgtcc ctggttatcc atgttttttt accgtccttt gccgaaaccg
     1981 tcagggatgg gcagagagag ggcattaccc tgtgacggaa atatcaagtc ccaacttttt
     2041 cctgacgttt aatcaaaaat cgtttgataa ccttgtccat gctcccattt gcggaattgg
     2101 gtcgagaata gtcacctaag atctgggtac gatcggtcac agcctcttcc tccgttggcg
     2161 cagacagtgg gggtcttcgt gtttccggtc gattcatcat tttccctcct ctcgaattag
     2221 tgatgggttc aatccaagcg tcagaatctt ttggtatcga tctgttcaga cgcgcaacac
     2281 gtattcgtcg tcactcgctt caatgaggaa aggatcaacc ctcgtctccc tcaagcgttc
     2341 ctagcataat cgataccatc tagccgctag agatttccgc acccaaccta tgcccccaaa
     2401 ctcgtgactg gagtttgcat tggtcgtcaa gggttataat ttttcgaaga ggattaggac
     2461 gaggagagga gcttaaatgg gcgttttcgc actgaacgct cacgcgaatt cactcatata
     2521 ggcgaaacac aggcttgaat acccatgcgg ccacggcgtg acatgacgta tcaaaaaccc
     2581 caccgggtcc aggacaaacg atcgagcaat tatccatgtg agggagagac tcaggcatcc
     2641 aaccccttca actacgcgtt catacggatc tgtcaaacat aatcaatagt ctaatttgtg
     2701 cgaaagtatt cctaaagcgg caggaaaagc aggaggtaaa ctggaacgca tggaaatccg
     2761 gagaagccca aacctacact cttttcacac attcatgaaa taatcaaaag gattctgagt
     2821 ttcatccatt ggcgccaatg agaagtaaca gcacagtcaa ttaaaaatga atgttcggat
     2881 ttggggttat gtttctgaag aaagtggaat ccacgcagtg atcatagtac ctttgccttt
     2941 ggaggctttc accttaaatg tgccctgcac cacacgaatt cgttccttca tcccgatcag
     3001 acctaatccc tgactgcccg ggcgaacagc ctcagggata aatcctttac cattgtccgt
     3061 aataatgagc tgaaggccgg cctccaaccc catcacctcc acgagcaccc gagtggcatc
     3121 cgcatgcttc gccacatttc gtaaacattc ctgagtcacc cggtacacac acgacgcgat
     3181 gtcctggggt aaagcatgcg gcacatcccg gggtcgaaac gtgactgaaa tattctccca
     3241 ttgagaaaat tctctaatag aggactggag tgcgacgacc aaccccaggt catccaaaat
     3301 agaaggatgc aactggtagg ctaaatgccg gacgctatcg gaaagcgagg acacttgatc
     3361 attcaggtgt tgaagagtct tttgcatggc attcgattct ggcagggtgt tttgaatgga
     3421 ttgtatctcg agggcgacca cggccaaccg ttgattcata tcatcatgaa gttcacggga
     3481 aagacggcgt cgctcatctt cctgggcaga aatgagttgg gcgccaagag cccgaagctc
     3541 ttcctggcta tgctcgagtt cccgctctcg gcgatgaaga gcttgttctg cctctttgcg
     3601 tgccgtgata tcccggacaa ttccggtaaa aaaggcctct ccatgcttgt gccaatatcc
     3661 cagagacagt tcaagaggga attcgctgcc gtcccttcga agccccttca gctcaaacat
     3721 ctccccctca acagtctttc cccccgctcg tgaagcccgt gcaattccat ggtgatgagc
     3781 caaacgaaac cgttcgggaa tgatacgctc taacgtttga ccgtacattt ctttcatcga
     3841 aaagccgaac aagctttccg ctccggcatt ccacagcatc accttcccct gactattcaa
     3901 cgaaataata ccctcatcag ttgattgcac caccgaatgt agctgttcct ggctttcccg
     3961 gagagccacc tctgcctgtt tacgctcaag aacttcgctt tgcaatgatt ggttggacct
     4021 acccaacgca aggcttaagg tcatcgatgc tatcgtcaca gtatgattac gtcgtaaggt
     4081 gacaaacatc atagtcacga aaaggaggct catggctccc atggccatgt ggtgttcatc
     4141 atccaggata aaaaattgaa cgaggagtgg ggtaatagtc gggagagtat aggccaggaa
     4201 cgctccttcc agggccgcat acacagcggt cgccccggcg atcatgcccc ctaacacaaa
     4261 ggccaaaaat atctgatgtg taatggaatt ttcaggaaag aggaaggcac ccgttgtccc
     4321 ccagagcagg ccggaagcaa aggtcccgcc aagaaatctg cgcccccagc gatgggaatc
     4381 ctgaggattt cgagacccgt gcctgaattg ataccatagg aggctccagg ccccattgag
     4441 cacgatcagg caggaaaacc acgtaagcaa tatgccatgg gcaatcacat tccattgaat
     4501 aaaagttaga atagatccat tgaccaggga ggcgatgatc ccaaccggcg cgagcccata
     4561 gagttgctct acctgcttcg catacaagag atcgcgttcg tattcatcca actcataccc
     4621 tccacaacat tccaaagaac gttctaagac cctcttggat ttccaatgtc atgtagacat
     4681 tcgtggggga aattttgctt atctgtttag taataatctt actgtagaac tcttattaat
     4741 taatcacaag agtattgcac cactcaacct tagacggacg aacgtcagga ttatagattt
     4801 gcaaagaaca gcagaaggct ccccaagccc agcatcacca acccg
//
LOCUS       DAORAQ010000188         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2531_length_4810_cov_74.274448, whole genome shotgun sequence.
ACCESSION   DAORAQ010000188 DAORAQ010000000
VERSION     DAORAQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_4810_cov_74.274448"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="clpX"
                     /locus_tag="PKM72_12300"
     CDS             complement(<1..811)
                     /gene="clpX"
                     /locus_tag="PKM72_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNP61616.1"
                     /translation="MARQAKSEANLRCSFCGKNRDQVRKLIAGPTVYICDECVGLCNE
                     IISEDWQESRQEISAKLCKPVDIHQHLDQYIIGQDKAKKVLSVAVYNHYKRISANEIG
                     DVELQKGNILIVGPTGTGKTLFAQTLAHYLDVPFTIADATTLTEAGYVGEDVENIILK
                     LLQAADYEVERAERGIVYIDEIDKITRKADSPSITRDVSGEGVQQALLKLIEGTVANV
                     PPQGGRKHPHQEFIQVNTTNILFICGGAFVGLDSIIERRLNQKRMGFGADVQ"
     gene            complement(832..1461)
                     /gene="clpP"
                     /locus_tag="PKM72_12305"
     CDS             complement(832..1461)
                     /gene="clpP"
                     /locus_tag="PKM72_12305"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNP61617.1"
                     /translation="MLIPMVIEQTNRGERSYDIYSRLLKDRIIFVGAPIDDMFANVII
                     AQLLFLESEDPEKDIHLYVNSPGGSVTAGMGIYDTMQYVRSPINTICLGQAASMGALL
                     LTAGTKGKRFALPNARVMIHQPMGGFQGQATEIDIHAREILKIREKLNEIMAFHTGQP
                     LDKISVDTERDYFLSGEEAKAYGLIDEVIVRSQEKGHKDDTKSGSKSKD"
     gene            complement(1543..2868)
                     /gene="tig"
                     /locus_tag="PKM72_12310"
     CDS             complement(1543..2868)
                     /gene="tig"
                     /locus_tag="PKM72_12310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNP61618.1"
                     /translation="MKLEMTELGPVKRSLKIEVPEEAVKSEFQKVYAQLRREAKIPGF
                     RPGKAPIALLEKRYADLVEQDVVQRLVPDYYQRAVKEAGVVPILVDIPPLERMKVKSN
                     SPFTFTATVEIKPTIQLGDYRPPNPISLKRDARTVTDDQVDKALQSLREQHAQLDVVP
                     DGTPLREGLHAVLSIEGFVEDQPLEGTQKKGHLLHIGSKSPILGVELDEYLIGKQAGD
                     MIDIPQPFPANHPDSHLAGKTMVLKVTVDAVKEQKLPELDDEFAKDCGDYDSLEHLKT
                     TINTQLDNFLKQDLEEGYKDQVIERLLQMHHFEIPEVLIERELQTLIRQKMLKNHRHT
                     HREDDVEEPLRLQEEAKRLQQELHPEAKRRVKLSLILDAIVQKEGLHVTEVEVNAEIQ
                     KLATSLKLPVGEIQRMVEAGGQGAQEEFRERMKAEKALQFVYQNAVIQG"
     gene            complement(2922..3004)
                     /locus_tag="PKM72_12315"
     tRNA            complement(2922..3004)
                     /locus_tag="PKM72_12315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2969..2971),aa:Leu,seq:tag)
     gene            3400..3645
                     /locus_tag="PKM72_12320"
     CDS             3400..3645
                     /locus_tag="PKM72_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61619.1"
                     /translation="MNLKQLYQDIYQKYLEKKDDHQGYDFQESPVGSATPPSPTFADT
                     IKWWTLNRPSRLQKIRQDRDQRQQDIINIQSPPPANR"
     gene            3671..4477
                     /locus_tag="PKM72_12325"
     CDS             3671..4477
                     /locus_tag="PKM72_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61620.1"
                     /translation="MQSFIQATGRLYHQALFASIQSFTRAWILIPVLLAYALGLILVT
                     SLIAPLGMIGGFILGALNALVIGSTLSLTEQAVLGARRVSWPDILPSMGQYFWEVISV
                     GFMVWFPLLFLEMGLQTNPYQPLIATAVFFLIFVLLNPVPEVIYQSRNGSALEVVRES
                     YEFVVENWIEWFLPVAIVFGPFGLTFFFQMSSQAGRRGGLDFWHLITLPFSVLSEWLS
                     LLGVSSDVSFVIVLGLTPIVTVFTLLFRGHLFAALHRSSRRQRMFQSRIS"
BASE COUNT         1169 a         1294 c         1042 g         1305 t
ORIGIN      
        1 tctgcacatc ggcgccaaag cccatccgct tttggttcaa cctccgctca atgatagaat
       61 ccaaccctac gaatgccccg ccacaaataa ataaaatatt ggtcgtattg acttgaataa
      121 actcctgatg agggtgtttt cttccccctt gaggcggaac attggctacg gtcccttcaa
      181 tgagttttag caacgcctgc tgaacaccct caccggagac atcccgcgta attgaagggc
      241 tatcggcctt gcgggtaatt ttgtcaattt catcaatgta cacaattccc cgttctgcac
      301 gctcaacttc atagtctgca gcctgcagaa gtttcaagat aatattttca acatcttcac
      361 cgacataccc ggcttctgtc agcgtcgtcg catccgcaat cgtaaacgga acatccaggt
      421 aatgggctag tgtctgggca aataaggttt ttcccgtccc ggtcggcccg acaatcagaa
      481 tattgccttt ttgaagttca acatccccga tttcgtttgc tgaaatacgc ttataatggt
      541 tatagaccgc aaccgagaga accttttttg ccttgtcctg tccgattatg tattgatcaa
      601 gatgttggtg gatatccacc ggcttgcaca gcttggcgga gatctcctgg cgagactcct
      661 gccaatcctc ggaaataatc tcattgcaca agccaacgca ttcatcacaa atatagaccg
      721 tcggccccgc aatgagtttt cgaacctgat cacggttctt gccgcagaag gagcaacgaa
      781 gattggcttc ggacttcgct tgtctcgcca taaaaagtgc ctcctttgcc cttaatcctt
      841 actttttgaa cctgacttcg tgtcgtcctt gtgccccttc tcctgacttc ggacgatgac
      901 ctcatcaatt aatccgtaag ccttggcttc ttcccctgat agaaaataat cccgttccgt
      961 gtctacagaa attttatcca gaggctgccc tgtgtgaaag gccataattt cattcaattt
     1021 ttcgcggatc tttaaaatct cgcgggcatg aatatcaatc tccgtcgctt ggccctgaaa
     1081 tcctcccatc ggttgatgaa tcatgacccg tgcattagga agagcgaaac gcttaccttt
     1141 agtccctgct gttaataaca aggcgcccat gcttgccgct tggcctaaac agatggtgtt
     1201 gatgggcgat ctcacatatt gcatcgtatc atagatcccc atcccagccg tcacacttcc
     1261 gccaggtgaa ttgacataca aatgaatgtc cttttccggg tcttcggatt ccaagaacaa
     1321 cagttgagcg atgatgacat tcgcaaacat atcatcgatc ggcgctccaa caaaaatgat
     1381 ccgatctttt aagaggcgcg aatatatatc atatgaccgt tcacctcgat tcgtttgttc
     1441 aataaccatc ggaatgagca ttccctgaga cctcctcgtg ttgtaaatag gtatcaaata
     1501 cagatatcag aacaggtgta ttcagaagga tcgcaccacg cgttacccct gaataaccgc
     1561 attttgatag acaaactgca aggctttttc cgccttcatc cgctcgcgaa attcttcctg
     1621 agccccctgt cctccagctt cgaccatgcg ttgaatttca ccaaccggga gctttaagga
     1681 ggttgccagt ttttgaattt cggcgttcac ctcaacctca gtcacatgca gtccttcttt
     1741 ctggacgatc gcatccagaa tcaagctcag cttcacacgc cgtttggctt ccggatgcaa
     1801 ttcttgttgg agtcgcttgg cctcttcttg taatcgtaag ggctcttcca catcatcttc
     1861 acgatgagta tggcgatggt ttttcagcat tttttgacga ataagcgtct ggagctcccg
     1921 ttcaatcaaa acctcgggaa tttcaaagtg atgcatctgc aacaagcgtt caatcacctg
     1981 atctttatac ccttcttcaa gatcctgttt caggaaatta tccaattggg tgttgatggt
     2041 ggttttgagg tgttccagtg aatcgtagtc gccgcaatcc ttggcgaatt catcatccag
     2101 ttccgggagt ttctgctctt tcacggcatc aacagtcacc ttgaggacca tcgtctttcc
     2161 ggcaaggtgc gaatcaggat gattggcggg aaagggttgc gggatatcaa tcatatcccc
     2221 ggcctgtttt ccgatgagat actcatccaa ctccacaccc agaatcgggg acttggaccc
     2281 aatgtgaaga agatgcccct ttttctgggt cccttccaaa ggctggtcct ccacaaaccc
     2341 ctcaatagac aacaccgcat gcaatccttc acgaagaggc gttccatcag gaaccacgtc
     2401 aagctgggca tgttgctccc gtaggctctg gagagcctta tcaacttgat catctgtgac
     2461 cgtccgtgca tcccgtttca aggaaatagg attcggtggt cgatagtcgc ctaattgaat
     2521 agtgggcttt atttccaccg tcgccgtaaa agtaaatgga ctattggact tcactttcat
     2581 gcgttccagt gggggaatat caacgaggat aggaactacc ccggcttcct tcaccgctcg
     2641 ctggtaataa tcgggcacca accgttgcac gacatcctgc tccacgagat cggcataccg
     2701 tttttctaat aaggctattg gcgctttccc tggtcgaaat ccaggaattt tggcttcacg
     2761 cctcaactgg gcatacacct tttgaaattc gcttttaacg gcttcttcgg gaacttcgat
     2821 ttttaacgac cgcttgaccg gcccgagctc tgtcatttct aacttcataa gaccattcat
     2881 ccttgtaaaa gggcacaata aagattatag atgttaagaa ttggtgcgag aggggggact
     2941 tgaaccccca cggtttaccc acgagatcct aagtctcgcg cgtctgccat tccgccactc
     3001 tcgcccgtaa ccgtttttgc ttcactccat agcttctata agatgttctt cctctggaat
     3061 gatcagggga aataagaccc tgtcttctac ggaccctccc tattataaaa cgtctatctc
     3121 cttcaggcca tcctaaaatt caggtaaaga acacagtcct ccggagagcg ccgcaactta
     3181 cacccggatc aagcggggaa atattccgat ggatatgatg atgaaggatt caccagaacc
     3241 cccctcaaac caaatcatag ccccaataat tatattagaa cagacaaagg accacccgcg
     3301 caccctcaaa ggaaacgaac gctacagagg tgatttctca accctcctat ggataacccc
     3361 tcttgatcgg ctcgaatgag ttgggtaagg taacactcta tgaacttaaa acagctctat
     3421 caagatattt atcagaaata tcttgaaaaa aaagatgatc atcaggggta tgactttcaa
     3481 gagtccccgg tagggtccgc aactccgccg tcacccactt ttgcggatac catcaaatgg
     3541 tggacattga atcgtcccag tcggctacaa aaaatcaggc aagaccgaga tcaacggcaa
     3601 caggacatta tcaatattca atccccgcct ccagccaacc ggtaagatcc gcggagcatc
     3661 ttctcgtttc atgcagagtt tcattcaggc cacaggtcgc ctttaccacc aagccctgtt
     3721 cgcctcgatc cagtccttca ctcgggcgtg gatcctaatc cccgtcctcc ttgcctatgc
     3781 cctaggcctc attctggtca cctccctcat tgctcccctt ggaatgatcg gagggtttat
     3841 cctgggtgcc ctcaatgccc ttgtcatcgg atctaccctt agtctaaccg aacaggctgt
     3901 cctgggggcg cgtcgtgtga gttggccgga tatcctgcca agtatggggc aatatttttg
     3961 ggaagtcatt tctgttggat ttatggtgtg gtttcccttg ttattcctcg aaatgggtct
     4021 ccagaccaat ccctaccaac cactgattgc cacagccgta tttttcctaa tttttgtgct
     4081 actcaatcca gtaccggaag taatttacca gagccggaac ggatccgccc tcgaggttgt
     4141 tcgggaaagt tatgaatttg tcgtcgaaaa ttggatcgaa tggtttttac ccgtggccat
     4201 cgtcttcgga ccattcggcc tgacattttt ctttcaaatg tccagtcagg cgggaaggcg
     4261 tggggggtta gacttctggc atctcatcac gttgccattt tccgtgttat ccgagtggct
     4321 ctctttgttg ggggtgtctt ccgatgtgag ttttgttatc gtcctcggac ttactcccat
     4381 tgtgacggta ttcacgctcc tctttcgagg ccatttattt gccgccctgc atcgatcttc
     4441 ccgacgccaa cggatgttcc aatcccgaat ctcctaagta agaaaggaag aagatagcat
     4501 gaaacataag gagtgaggag ccagaggcag ggtggatacg cgctctttat ccctcttcgc
     4561 tctactctaa ctattccctt cacttgcttg tcgctctcag acccccatgg taagatggca
     4621 acctttcacc aaacagatcc tcggtgaaga agacaacaaa gatggggttg atagatcccc
     4681 tccaaaagac aaacggcttc atcagaggtt caatcgccgc aaagtagata cccattcgcg
     4741 gagaggtgcg agagtggccg aatcggcgtg actggaaatc acgtgtgcca gcaatggtac
     4801 cgtgggttcg
//
LOCUS       DAORAQ010000189         4801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2537_length_4801_cov_64.496629, whole genome shotgun sequence.
ACCESSION   DAORAQ010000189 DAORAQ010000000
VERSION     DAORAQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_4801_cov_64.496629"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PKM72_12330"
     CDS             complement(<1..1063)
                     /locus_tag="PKM72_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNP61621.1"
                     /translation="MGLVHGLHFNNLRGDIYGGMVAAVVALPLALAFGVASGLGAIAG
                     IYGAIFVGFFAAIFGGTPAQVSGPTGPMTVVMAGIVTLFMGNPGLALMAVILGGGFQI
                     LLGLSKVGQYIALVPYPVVSGFMSGIGCIILILQLGPMVGHAANPEGVVATLKAIPGF
                     FGNPVWDAAMTSALVLAIVYFTPTRMAKLVPPSLLALLVVSPLAYVFLPDAPIIGEVP
                     QGFPTPLLPTLTWDTLQIVLESAITLALLGAIDSLLTSLVCDNMTRTQHDPDRELIGQ
                     GIGNMVAGVFGGIPGAGATMRSVANIRSGGRTPISGALHAVILLAILLGLGPLAEKIP
                     LAVLGGILFKVGIDIIDWRF"
     gene            complement(1247..1822)
                     /locus_tag="PKM72_12335"
     CDS             complement(1247..1822)
                     /locus_tag="PKM72_12335"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNP61622.1"
                     /translation="MTRQFFFILLFSILISLGIGVGIGGKITPRDGIPIRVVADYLHA
                     VLQADRTFYTQHIVERMEDMLIVTATENWREERTLPLPAQFFKEASRGLQAPGKPFRY
                     RLMGLWPLNPENSPHNDRERKALEQVVKYGEVTEQEIQVDNQSYYQVIFPDRAVSRAC
                     VNCHNAHQESPKRDFKLNDVMGGLEILISLD"
     gene            complement(2682..4070)
                     /locus_tag="PKM72_12340"
     CDS             complement(2682..4070)
                     /locus_tag="PKM72_12340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNP61623.1"
                     /translation="MRATIFVTDDDQNVCSALSRRLVKKGHLVRSFHSGASLIEALEH
                     EIPDLLFLDLKMPEMDGLETLRQIRQTIPKTMIFMLTAYGSVEDAVEAMRLGAYDFLI
                     KSIDFSTVEPALHRAITFLELRRRIEFSASEKQRQYSWEHVIARSPAMTKVIEQLKIL
                     DAQDAPLVFLQGEVGTGKEFLARILHYNSRKHLGPFVTVSCNERSGSLLEPQIFGYER
                     GAFSGASQSMPGALEHSDGGTLFVDEIEHLSIPVQGSLAEAIHTRSFCRMGGFDRLPI
                     NNRLVVTSATPQDQTGYKDIFHPELLSLLQKRQLFIPPLRERKEDIIPLVIKTIQVYG
                     EEIGRPKLDIDSSIPPLLDTYPFPGNIRELEAMIRRAVLCSQGPVLTSLDFYSQHPGG
                     GDGLSPNIGRVLFEIGQHDLQDIQVMVIDEVLRFTKQDRERAARYLQISKQTLDDYIQ
                     LRKNFSHTTKRS"
     gene            complement(4357..4608)
                     /locus_tag="PKM72_12345"
     CDS             complement(4357..4608)
                     /locus_tag="PKM72_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61624.1"
                     /translation="MTDNCVEPTNTISGTQLLNTKIPEVPYGYRLCVNSLSEDMPMNA
                     LTKAWLFFAILGLAIGIYLAETYNYIEMVEPLRYGWEPW"
BASE COUNT         1323 a         1056 c         1201 g         1221 t
ORIGIN      
        1 ggaagcgcca gtcaataata tcgatgccaa ctttgaaaag gatgccgccg agaaccgcta
       61 agggaatttt ctctgccagc gggccaagtc ccaatagaat cgccaataaa ataaccgcgt
      121 gaagcgcgcc ggaaatcggt gtccggcctc cggatcggat attggccacc gaccgcatgg
      181 tggctcccgc tcctgggatt cctccaaata cccctgcgac catatttcca atgccctggc
      241 caataagctc gcgatccggg tcatgttgag tccgggtcat gttatcgcaa accaggctgg
      301 ttagcagact gtcgatcgct cccaataatg ccagggtgat ggctgattcc aatacgattt
      361 gcaaggtgtc ccatgtgagt gttggcagga ggggagtggg aaatccctgg gggacttctc
      421 cgatgatagg ggcatcagga aggaaaacat aagcgagcgg ggaaaccacg aggagggcca
      481 ataacgaagg aggcactagt tttgccatcc gagtgggagt gaaatataca atggccaaga
      541 cgagggcgct tgtcatggca gcatcccaca ccgggtttcc aaaaaatccc gggatagcct
      601 tgagcgtcgc gacgacaccc tcagggtttg ccgcatggcc taccatggga cccaattgca
      661 aaatgagaat aatgcagccg atgccgctca tgaacccgga aacgaccgga tacgggacca
      721 gcgcgatata ctgccccacc ttggaaagtc ccaacaggat ttgaaagcct ccgccaagaa
      781 tcaccgccat caaggccaaa ccaggattgc ccatgaagag tgtgacaatt cctgccatca
      841 ccacggtcat agggcccgtg ggaccggaaa cttgagcggg ggtgcctccg aagatcgcag
      901 caaaaaatcc gacgaatatg gccccgtata taccggcaat ggctcctaat cccgaggcga
      961 cgccaaaagc gagtgccaac ggtagagcta cgacagcggc taccattcct ccgtaaatat
     1021 caccacggag gttattgaaa tgtaacccgt gaacaagccc catagggcat atccttgagg
     1081 agtaataaat gatgaaggct ggctgggagg agatttcact ctatctcaag gttcttcgtc
     1141 aaggggcgta agcaaggggt tttcctagaa aagacaaggg ggaaataagg aggattgggg
     1201 gaaaggctga tgccggaatg ggctctaaac tttggtacag gaaaggttag tcaagggaaa
     1261 ttagaatttc caatcccccc atgacatcat ttaatttaaa gtcccgtttg ggactttcct
     1321 ggtgggcatt atggcagttg acgcaggctc ggcttacggc tcgatcagga aaaataactt
     1381 gataataaga ctggttgtcc acctggattt cttgctcggt cacttctcca tatttaacaa
     1441 cttgttccag ggctttacgc tcccggtcat tgtgaggtga attctccgga ttcaaaggcc
     1501 aaagacccat caaccggtaa cgaaacggtt tcccgggggc ctgtaatcca cgagaggcct
     1561 ccttgaaaaa ttgtgcgggc aacggcaatg tccgttcctc tcgccaattt tctgttgctg
     1621 tgacaatcag catgtcttcc atccgttcca cgatatgttg ggtataaaag gtccggtctg
     1681 cttgaaggac ggcatgtaaa tagtctgcga ccacacggat aggaatgcca tcccgtggtg
     1741 taatttttcc cccgatgccc acaccaattc ccaaggaaat gagaatggag aaaaggagta
     1801 tgaaaaagaa ctgacgagtc ataagtctat cattaccttg ttggtctaca tcctctctgt
     1861 cctgcacatt cttcgcttga gggtgaatta tgcaaaacta taacaggaga tctacaagac
     1921 ctcatacttt tacatacatg aaattgtagc tatggcaatc ggatgagggg caacggcgta
     1981 ggaaggcaat caaccgtgga gaattaaaaa aatcggaagg ggttcggact taatccaaac
     2041 ccagccgatt tattaattgg ataacaatga tggtgaaaat tactatgaga gggacatgtt
     2101 ccatgttaga acctcgagag taccgcccaa gtgataagta aaattagggt aaagccttcc
     2161 attctgttca ttctcctgat atttctggat attgtttgaa taaatctgca aggtaaatgc
     2221 caatagatgt tatcttgatt gttattgaaa aattcaaaaa tatcattgac ttatatgttt
     2281 taatacgtag ttcgtctatt attatcattg gaagtgagcg aatttattaa gtaaacactg
     2341 tgcgttatcg ctcgattttt gaagttagta gagtcaaatg agtgagaggt actaaacaca
     2401 cgttggttat ctcatgggag ttggtcgagc agggtttggc ggattagagg aaacgcatca
     2461 ttcgtgaatg aaagagcaca aaagtgggag gattaagtag cgattcagaa agttactaaa
     2521 aaaatttgtt acacaatcgg tggaggcagg gtcatgcttg tcaaaggctt gagccctgag
     2581 tttaagccta gggagttaat ggaggaattt tttccccgta tctagagaag aatgtgtcga
     2641 aagggaaagg cgaatgaatg aggagatccc cggctcatgc cttaggacct ttttgtcgta
     2701 tgagaaaaat tttttcgtaa ttggatgtaa tcgtccagtg tctgtttaga aatctgaaga
     2761 tagcgagccg cccgttctct gtcctgcttg gtgaatcgca ggacttcatc aatcaccatg
     2821 acttgtatgt cttgtaggtc gtgttggcca atctcaaaaa gcacccgacc aatatttggg
     2881 gagagtccgt ctcccccgcc aggatgttgg gagtaaaagt ccagagaagt aagaactggt
     2941 ccttgggagc acaataccgc cctgcgaatc atggcctcca attctcggat gttcccaggg
     3001 aatgggtaag tatccaatag cggggggatg gaagaatcaa tgtcgagttt gggacggccg
     3061 atttcttcac catagacctg aatggttttg atcaccaagg ggatgatgtc ttctttgcgt
     3121 tcacgaaggg gaggaataaa gagttgccgt ttttggagaa gagacagtaa ttctggatga
     3181 aatatgtcct tgtagcctgt ctgatcctgt ggggtcgcac tggtcaccac aagtcgattg
     3241 ttgatgggga gacggtcaaa cccacccatt cgacaaaatg aacgagtatg aatggcctcg
     3301 gccaatgatc cttgaacagg aatggataaa tgttctatct catcaacaaa tagtgttcct
     3361 ccatcagaat gttcaagtgc tccgggcatg ctttggcttg caccactgaa tgctccgcgt
     3421 tcatatccaa atatttgtgg ctcaaggagg gatccactcc gttcattgca actgaccgtg
     3481 acaaacgggc cgaggtgttt gcggctatta taatgaagaa tgcgtgccaa gaattccttt
     3541 cccgtcccta cctccccttg aaggaacact aagggggcgt cctgagcgtc gagaattttg
     3601 agttgttcaa tgaccttggt catggctgga ctcctggcga ttacatgttc ccaggaatat
     3661 tgccgttgtt tctcggaagc agagaactca attcgacgcc ttaactccag gaaggtaatg
     3721 gctcgatgta aggctggctc aaccgttgag aaatcaatgg atttaattaa gaaatcatac
     3781 gctccgagcc gcattgcctc taccgcatcc tcgacgctcc cataagctgt caacatgaaa
     3841 atcatggttt taggaattgt ttgtcgaatc tgtcgtaagg tttcgagtcc atccatttct
     3901 ggcattttaa gatccaggaa cagcaaatca ggaatttcat gttccaatgc ctcaatgagc
     3961 gaggctcctg aatggaagct tctcacgagg tgcccttttt tcactaaccg acgacttaat
     4021 gcagaacata cattttgatc atcgtcggtc acaaaaattg ttgcacgcat agcagtcctt
     4081 gtttaggaga tcaatttaga agttgtaccg ccactttatt gtactttccc atctttggtc
     4141 attgatatca accgcagagg agttctggaa gcagtgattt ttttaggtat ggggtctttt
     4201 gtaataattg gaaagagagc cccagccatc gtcctgaatt caaggggcat tggaaaacta
     4261 tcaggactga ctttcagaag atctcgacct caatgaacca tgaatggggt tgaggcaatg
     4321 tgtttgaatg tcgtatatcc tctttcgtag cagcactcac cacggctccc agccatagcg
     4381 cagaggttca accatttcaa tgtagttgta cgtttcggcc aggtagatgc cgatcgccaa
     4441 cccgagaata gcaaaaaaca gccatgcttt cgttaaggcg ttcattggca tgtcctccga
     4501 aaggctattt acgcaaagtc tatacccata gggtacctct ggaattttgg tgttcaacag
     4561 ttgagtccca ctaatggtat tcgttggttc cacacaatta tcagtcatgt cggtatcaat
     4621 aatttttgcg ccttattgct gaacacaatc agccagcgtg cgatgatgca caggggatta
     4681 tgtgtgaaat ggcaccagtt ttcaaatttt ggtcactatg catgaaggca gaagccaggg
     4741 gaaatttgat aggaacgtaa ggcaaattgt gaaaatgaaa attattatga ccggcttctg
     4801 t
//
LOCUS       DAORAQ010000190         4797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2541_length_4797_cov_69.420919, whole genome shotgun sequence.
ACCESSION   DAORAQ010000190 DAORAQ010000000
VERSION     DAORAQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_4797_cov_69.420919"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKM72_12350"
     CDS             <1..212
                     /locus_tag="PKM72_12350"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61625.1"
                     /translation="LLSFGAQAALLSGSGATVFGIFRTHEEARQASTRLRRDTSWRIF
                     DIPMGSTDLPHDAIHVGSSSQASKM"
     gene            324..1265
                     /locus_tag="PKM72_12355"
     CDS             324..1265
                     /locus_tag="PKM72_12355"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNP61626.1"
                     /translation="MLRDLKLITGNSNLALAREISQYIGQDLSQATVTTFSDGEIRVR
                     IDENVRGGDVFLIQSCCHPVNTSIMELLLLIDAVKRSSASRITAVIPYYGYGRQDRKD
                     QPRVPISAKLIADLITAAGANRILTMDLHAGPIQGFFNIPVDHLYAMPVMLDYIKKQN
                     VSDLVIVSPDAGGVERARAFAKRLNSSLAIIDKRRELPNQAEVMNIIGDVQDKHALLF
                     DDMIDTAGTIVQTAQTCMDNGALSAWAGSTHAVLSGPALERLQSSCLREVMVTNTIPL
                     NGKDQQCPKLKTLSVAPLLGEAILRIHREESVTSLFV"
     gene            1314..2012
                     /locus_tag="PKM72_12360"
     CDS             1314..2012
                     /locus_tag="PKM72_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNP61627.1"
                     /translation="MKYELEVEKRDQAGKGVARQLRRQGKIPGVLYGGGKSEFVAMDH
                     KTARSLVISQVGHTGLLTVRISGAQERIAVLQDHQIDPITGAILHVDLLEVSMKKVIR
                     VKVPVTVIGEVPLGVKEGGILHQVLRELHIECLPDQIPDHIEIDASGFGIGDGVYVKE
                     VAVPAGIKILDDEDLMVAHVATKMSEAKLESLLAREAAEGGAPVATAEKAAGEGAAAG
                     AVAASDSKSKEGKK"
     gene            2025..2594
                     /gene="pth"
                     /locus_tag="PKM72_12365"
     CDS             2025..2594
                     /gene="pth"
                     /locus_tag="PKM72_12365"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNP61628.1"
                     /translation="MVGLGNPGESYKHTRHNIGWMVLEQAADRWNVALAKQEHGFLGK
                     GEVKGHPVLLLLPMAWMNQTGVVIRSVIQDLSQESPNLIVVHDDLDLSLGAIKIKTRG
                     GAGGHNGLRSVLSCVGTEEFSRVKVGIGRPQNNDSLANFVLSPFLSEEWEKITSILPK
                     AIDSLECLICEGPDIAMNRFHVRSSEEGL"
     gene            2805..3164
                     /gene="rpsF"
                     /locus_tag="PKM72_12370"
     CDS             2805..3164
                     /gene="rpsF"
                     /locus_tag="PKM72_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNP61629.1"
                     /translation="MKLYESLCILRPVQAETENERVIEKMKEVLGQAGATILNFDNSG
                     KKKLAYDIQHERKGTYITVQFEGPPTVVFELERFQRMEDQVMKFMTVRLNPSDLVAVA
                     ETEAEASGEDAGDGGIQ"
     gene            3148..3558
                     /locus_tag="PKM72_12375"
     CDS             3148..3558
                     /locus_tag="PKM72_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNP61630.1"
                     /translation="MVGFNKVILIGNLTRDPELRYTPSGTPVVNFPLAVNRRYRQGDE
                     PKEEVCYVDIVVFGRQAEHCGQYLNKGDGAIVDGRLQQRRWETDDGQKRSKHEVVAQT
                     VTFLPKKSSSGSSGSGVSEESPYDSEEYYPDPMP"
     gene            3582..3809
                     /gene="rpsR"
                     /locus_tag="PKM72_12380"
     CDS             3582..3809
                     /gene="rpsR"
                     /locus_tag="PKM72_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNP61631.1"
                     /translation="MEKGRLFQRKRVCRFCFDKTPLDYKDANLLRNFLTERGRIIPRR
                     TSGNCLRHQRKLTQAIKRARHVAILSFAEEH"
     gene            3866..4486
                     /locus_tag="PKM72_12385"
     CDS             3866..4486
                     /locus_tag="PKM72_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNP61632.1"
                     /translation="MSIIFNLLDVPPAGLQIHHEVEPSALLLSSYEGSPIGSLSCHGE
                     LFLTGELSAYFQGKISGRMARECVRCLDQFEEDLSLSCEVEFKKAVKAVKVIVPHRDK
                     GKGVKGGDKPVEEDANEEDVYPISGNEIDLLPAIREQVILASPLQSLCNKNCLGLCQV
                     CGNNLNDGICGCCTPVTDSDPVPNFQQTISKKNPSKRSPASVRRGS"
     gene            4525..4707
                     /gene="rpmF"
                     /locus_tag="PKM72_12390"
     CDS             4525..4707
                     /gene="rpmF"
                     /locus_tag="PKM72_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNP61633.1"
                     /translation="MANPKHKISKARRDKRRSHLKLTPPNLSTCPQCHEPKPSHTTCL
                     NCGTYKGIAIISVEEG"
BASE COUNT         1244 a          971 c         1297 g         1285 t
ORIGIN      
        1 ctttgctttc ctttggggct caagccgcac tattatcagg tagtggagcc accgtgtttg
       61 ggattttccg cactcatgag gaagcccggc aggcttccac caggttgcgc cgtgatacta
      121 gttggcggat ctttgatatc ccgatgggtt cgacggattt accccatgac gcaatccatg
      181 ttggctcctc ttcccaggct tccaaaatgt aaattctgtg atttgagttg acaagccgct
      241 gattgtagaa ttacctttgc tctgcttttt tccagaatat ctgctacggt tcaggttcac
      301 ctaggcaagt tgagactacc accatgctcc gagatttaaa attgatcacg ggtaactcca
      361 accttgcgtt ggccagagaa atttcccagt atattgggca ggatttgagt caggcgacgg
      421 ttacgacttt tagtgatggg gaaattaggg ttagaattga tgagaatgtg cggggagggg
      481 atgtctttct cattcaatcc tgctgccacc cggtgaatac ctcgatcatg gagttgctgc
      541 ttctgatcga tgcggtcaaa cgatcatcag catccaggat tacagcggtc attccttatt
      601 acggatatgg gagacaagat cgaaaagatc aaccccgagt gcccattagt gccaaattaa
      661 ttgcggattt gattacggcc gccggcgcaa atcgcatttt gactatggac cttcatgccg
      721 gacctattca gggattcttc aatattcctg ttgatcacct ttatgcaatg cctgtgatgt
      781 tggactacat taaaaagcaa aatgtttccg atttggtgat cgtttcccct gatgctggag
      841 gagtcgagcg ggcgagagca tttgccaaac gactgaattc ctccctcgcg attattgata
      901 agcggcgaga attgccgaat caggctgaag tgatgaacat tattggtgat gtgcaggata
      961 aacacgcgtt attgtttgat gacatgattg atacggccgg aaccattgtt caaacggctc
     1021 aaacttgtat ggataatgga gctctgtcgg cttgggctgg gagtactcat gcagtcctgt
     1081 ctggtcccgc tctggagcgg ttgcaatcgt cttgccttcg ggaggttatg gtgacgaaca
     1141 ccattccttt gaatggaaag gatcagcaat gtccaaagtt gaagaccctc tcggttgcgc
     1201 ctttgttggg tgaggcgatt ttgaggattc atcgtgaaga atcggtgacg tccttgtttg
     1261 tgtaattagc taaggaattg ttcttttact ccattttaga agaggtcaag acgatgaaat
     1321 atgaacttga agtagaaaaa cgggatcagg ctggaaaagg cgtggcgagg caattgcggc
     1381 gacaagggaa aattcccgga gtcctttatg gagggggaaa gtctgaattt gtggccatgg
     1441 accataagac agccagaagt ctggtgattt ctcaggttgg ccatactggg ttgctgactg
     1501 ttcggatttc cggggcacaa gaacggatag cggtactcca agaccaccaa attgacccta
     1561 ttaccggggc aattctacac gtggatctcc ttgaagtttc gatgaaaaag gtcattcgcg
     1621 tcaaagttcc ggtgacggtt atcggagaag tccctcttgg tgtgaaagag gggggtatat
     1681 tgcatcaggt gttgcgcgaa ttacatattg agtgtttgcc ggatcaaatt ccagatcata
     1741 ttgaaattga tgcctccgga ttcgggatcg gtgatggtgt gtatgtcaaa gaagttgcag
     1801 ttcccgcagg tatcaagatt ttggatgacg aggatctcat ggttgcccat gtagccacga
     1861 agatgtctga ggcgaaatta gagtcccttc tggcccgtga ggcggctgag ggaggggctc
     1921 ctgttgccac tgcagaaaaa gccgcgggtg aaggtgcggc cgctggggct gtggcggcgt
     1981 cagatagtaa atcgaaagaa ggcaagaagt aacccttgca tctggtggtg ggacttggga
     2041 atcccggaga gtcgtacaaa cacacgcgac acaacattgg ctggatggtt cttgaacagg
     2101 cggcggatcg gtggaatgtg gctttggcca aacaggagca tgggttttta ggaaaaggtg
     2161 aggttaaggg ccatccggtt ttgcttcttc ttcccatggc gtggatgaat caaactgggg
     2221 tggtgattcg gtccgtcatt caggatttgt ctcaggaatc gcctaatctt attgttgtac
     2281 atgatgatct ggacttatct ctgggagcga tcaagatcaa aacccgaggg ggcgcaggtg
     2341 gccataatgg tctgcggtcg gttttatcgt gcgtggggac agaagaattt tccagggtca
     2401 aggttggaat agggcgtcct cagaataatg acagccttgc gaattttgta ctctcaccgt
     2461 ttttatcaga agagtgggaa aaaattacct caattcttcc gaaggccatt gattcgttgg
     2521 aatgcctgat ttgtgagggg ccagacattg ccatgaatcg atttcatgtg cgatcttctg
     2581 aggagggtct gtaagtattc gtggattggt cagttttcct ggcaacagcc ctactttact
     2641 tgaaaatttg tatcaagttt ttagtatgtg attgttcgga ttcttttcca gataagagtt
     2701 tgacactcca atttacccta tggtagcttg agtccccttt tgtggtacaa cacgattatt
     2761 ctgcctgaaa actagaaaaa tactgtcaga gggaaaggtt atgtatgaaa ctgtatgaat
     2821 cactgtgtat tcttcgtccc gttcaagcag aaacagaaaa tgagcgagtg attgaaaaaa
     2881 tgaaggaagt cctaggtcag gctggggcca ctattttaaa tttcgataat agcgggaaga
     2941 aaaaattggc ctacgatatt caacacgaac ggaaggggac ctatatcacg gttcaattcg
     3001 aaggacctcc taccgttgta tttgagttgg agcgctttca gcgtatggaa gatcaggtaa
     3061 tgaagtttat gaccgtacgg ctgaacccaa gtgatttggt tgccgtcgct gaaacggaag
     3121 ctgaagcaag tggagaggat gctggagatg gtgggattca ataaagtcat tttgattgga
     3181 aatctgacca gagatcctga gcttcggtat acacccagcg gcactcctgt ggttaatttc
     3241 ccgttagcgg tgaatcgccg ataccgtcag ggcgatgagc caaaagaaga ggtctgttat
     3301 gttgatattg tcgtgttcgg gcgacaggcc gaacattgtg ggcagtacct caataaaggt
     3361 gacggggcca tcgttgatgg tcgtctacaa caacggcgat gggaaacgga cgacgggcag
     3421 aagcgaagca aacatgaagt ggtagctcaa acagtaacgt tcttgccgaa aaagtcatcc
     3481 tccggtagct ctgggtcagg agtttctgag gagtctccct atgattcgga agagtattat
     3541 cctgacccaa tgccctaatt tttagccgag gagaggtttc tgtggaaaaa ggacgattgt
     3601 ttcagcggaa aagagtgtgc cgcttttgct tcgataaaac accattggat tacaaggatg
     3661 caaatctatt gaggaatttc ctcacggaac gcggacgcat tattccacgt cggacatctg
     3721 gaaattgctt gcggcatcaa aggaaattaa cccaagcgat taagcgtgcc aggcatgttg
     3781 ctatcctttc atttgcagaa gaacactgag gtcaacagtt ttctttgata tggggcagta
     3841 gagggtagag tgacggggtt gtgaaatgtc gataattttc aatctactgg atgtgccccc
     3901 ggctgggctt caaattcatc atgaggtaga gccatcggcc ttattgttat ccagttatga
     3961 gggaagccca attggtagct taagctgtca tggagagcta tttttgactg gggagctatc
     4021 agcatatttt caaggtaaaa ttagtggaag gatggccagg gaatgtgttc gttgcctcga
     4081 tcaatttgaa gaagatctgt cattatcgtg cgaggttgaa tttaaaaaag cggtgaaagc
     4141 ggtaaaagtg atcgtgccgc acagggataa aggcaaagga gtgaaggggg gcgataaacc
     4201 cgtcgaggaa gatgccaacg aggaggatgt ttaccctatt tcaggaaatg aaatcgacct
     4261 ccttccagct attcgagagc aagtcattct tgctagccct ctccagtcct tgtgcaataa
     4321 aaattgtctc gggttgtgcc aggtatgtgg gaataattta aatgatggca tttgtgggtg
     4381 ttgtaccccg gttacagact cagacccggt gccaaatttc cagcaaacaa tatcaaaaaa
     4441 aaatccatcc aagcgctctc ccgcttctgt tcgaagggga tcgtgaaggc atcagtcacc
     4501 tcttaattaa aaagttggac tattatggca aatcccaaac acaaaatttc taaagcccga
     4561 cgggataaac ggcgatccca tctcaagctc accccgccta atttgtctac gtgccctcaa
     4621 tgtcatgagc ccaagccatc tcatacgaca tgcttgaatt gtgggaccta taaagggatc
     4681 gccattattt cggttgaaga gggataaacg gcatttttct tctcgagcca gtcatctttt
     4741 agcaccaaac ccatgaaaat agccgtggac gcgatggggg gggatcatgg attatct
//
LOCUS       DAORAQ010000191         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2563_length_4765_cov_77.516985, whole genome shotgun sequence.
ACCESSION   DAORAQ010000191 DAORAQ010000000
VERSION     DAORAQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2563_length_4765_cov_77.516985"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKM72_12395"
     CDS             complement(<1..517)
                     /locus_tag="PKM72_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNP61634.1"
                     /translation="MSSDFPSAESDTFPIPDIVPIFPLPTTVFFPETYLPLHIFEPRY
                     REMVRQTSAQGDCIGMALLKEGWEDEYEESPPIYPIGCVGRIISSHKLPDGRFNIVLQ
                     GLQRCTFEEQEVSTPYRQAQIIPYAQRGAASLDIETRINLEQVTQEYLTWKKAHELCQ
                     VIASGKLTDSIL"
     gene            597..1841
                     /locus_tag="PKM72_12400"
     CDS             597..1841
                     /locus_tag="PKM72_12400"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNP61635.1"
                     /translation="MRGKGFVRNERSWYNRKHEVDEGLHPRDGYTGQEVRARSWWFEI
                     SFLIIFPLLGISSVFAETSNVGLHEFAPARPGYAYVFPRDHGSHKQFQTEWWYFTGHL
                     SSTNGRRFGYELTFFRRGIDSPDAWSNPSAWAMRHLYFAHFALTDEANEQFGFSEKLS
                     RAGIKKAGAEADRLNVWIDRWSVKTVAPDHRQFHLQAKAEDFSIDLTLESSKPPVTHG
                     TDGVSRKGQHPEDTSHYYSLTRLQTAGSVVVQGTPLAVNGVSWMDHEFGSADLSEGLV
                     GWDWFSLQLENDYDIMAYGLRRADGTFDPASSGTLVRPNGSSTSLSLEEIKVSVHGYW
                     TSPLSGARYPNQWTLSIPSEKVELNISPRMANQELVTRRSTDVTYWEGAVDVTGLWKG
                     EEIHGQGYVELTGYAEPYRPGQ"
     gene            2099..3127
                     /locus_tag="PKM72_12405"
     CDS             2099..3127
                     /locus_tag="PKM72_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61636.1"
                     /translation="MFRAHWIFVVFLMFGLAACGQRSDTIVSIALHPTKPNIVYVATD
                     EAVYKSSDTGATWTRFAGELARTRVICLALDPQLSATVFAGTMGDGTYKSPDGGRTWH
                     PYNSGIQKGTISAIVNQIVFNPLGTEMVYAGTTVGVFRSLDGGRHWTERMQGMTEVSF
                     VVTLAIDPQRPNVLYAGTTGGVYRTINATESWEKSSTGMVASDAKMASMALGVNGIVV
                     DPTNSDVVYAGTTDGLYKSTDQAEHWTKIGGSIQHAYISAIQLDPTNPSILYMATSDR
                     VQKSEDGGETWQPKIKGLEAASIRSLQMSPMDPRILYVGTNGGGLYRSMDAGESWIRL
                     PLVPASSE"
     gene            complement(3185..3352)
                     /locus_tag="PKM72_12410"
     CDS             complement(3185..3352)
                     /locus_tag="PKM72_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61637.1"
                     /translation="MASRETIGLDLITTSWTRHWSNTKDGAPTSHFGPTYGAKVMMDL
                     SFLLPESWRER"
     gene            complement(3339..3641)
                     /locus_tag="PKM72_12415"
     CDS             complement(3339..3641)
                     /locus_tag="PKM72_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61638.1"
                     /translation="MLQDIAVVLEIIQLPLAEIERLISLTIACRFHSSLTLVIILITC
                     LSWWISPQPARAEISAPGMHWGALNFPDQEPVFATGFSIFRFTEFNGEGERFNGIP"
     gene            complement(3746..4138)
                     /locus_tag="PKM72_12420"
     CDS             complement(3746..4138)
                     /locus_tag="PKM72_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61639.1"
                     /translation="MVNKRSPSLRKHVLIVDDEPAVRRTVRGALESSGIECAESENGA
                     AALAWLEENHVDLVLADYHMPIMSGLTLLERVNRTLNGRTPRVILLSGVIDEIHKHKA
                     MGLGAYAIIDKPCNFRELVEKVNEALDF"
     gene            complement(4431..>4765)
                     /locus_tag="PKM72_12425"
     CDS             complement(4431..>4765)
                     /locus_tag="PKM72_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61640.1"
                     /translation="FQQFPSDDDSLQPHNITFKLTYRVGSSNIECFIFSSPDGDLFSV
                     KIPPVTFIALQEALPSQTIPRMRVARLAVQQAIASGIPGIELLPGSKIYESVKSQLSG
                     SCLQKTQV"
BASE COUNT         1186 a         1061 c         1327 g         1191 t
ORIGIN      
        1 caagaatgga atccgtgagt ttgccagaag cgatcacctg acacaactca tgagcttttt
       61 tccaggtgag atattcttgg gtgacctgct ccagattgat gcgggtctca atgtccaggg
      121 aggccgcgcc cctttgggca tagggaataa tttgtgcttg tcggtagggc gtcgagactt
      181 cttgttcctc aaaggtgcat cgctgaagac cttgcaagac aatgttaaac cggccgtcag
      241 ggagtttgtg tgagctgata attcgcccca cacatccgat agggtaaatc gggggacttt
      301 cctcatactc gtcttcccag ccctctttga gcagggccat cccgatgcaa tccccctgag
      361 cggatgtctg tcgaaccatt tcacggtatc ggggttcaaa gatgtgtaat ggcaaatagg
      421 tttcagggaa aaaaaccgtg gtgggtagtg ggaaaatcgg cacgatgtcc ggtatcggaa
      481 aggtgtcgga ttcggccgat gggaaatctg aagacatgcg gtacgatagc ggagaatttt
      541 cgagaaagtc aacgtaaaat agtggctcca cgtgtttcaa taaaatgaga aaatccttgc
      601 ggggaaaggg atttgtacgc aatgaaaggt cgtggtataa ccggaagcat gaagtggacg
      661 aaggccttca cccacgcgat ggttatacag gtcaggaggt gagagcgaga tcctggtggt
      721 tcgagatttc gttcctgatc atattcccgc tactggggat ttcgtcggtt tttgcagaga
      781 cctccaacgt tggacttcac gaatttgctc ctgcccgccc tggatacgct tatgtattcc
      841 cgcgggatca tggcagtcat aagcagtttc agacagagtg gtggtatttt accggacatc
      901 tgtcgagcac gaatggtcga cggtttggtt acgaactgac attttttcgt cgcggaatcg
      961 actcacctga tgcctggagc aatccttctg catgggccat gcgacatttg tactttgccc
     1021 atttcgcctt gaccgacgaa gcgaacgagc aattcgggtt ttccgaaaag ctcagtcgtg
     1081 ccggaatcaa gaaggccgga gccgaagcgg atcggttgaa tgtgtggatc gatcggtggt
     1141 cggtgaagac ggtggccccc gatcatcgac aatttcatct tcaggcgaag gccgaggatt
     1201 tttccattga tttgacgctc gaatcaagta aacctcccgt tactcatggc acagatggcg
     1261 taagccggaa aggccagcat ccagaagata cctcccatta ttattcattg acccgtctcc
     1321 agacggcagg atcggtggtg gtacagggga caccgctggc agtcaacggc gtgagttgga
     1381 tggaccacga gtttggatct gctgaccttt cggaaggtct cgttggatgg gattggttca
     1441 gcctgcaact tgagaatgac tatgacatta tggcctatgg gcttcgccgt gcggatggaa
     1501 cgtttgatcc tgcctccagt ggaacgctgg tgagaccaaa tggatcgtcg acatcccttt
     1561 cccttgaaga gattaaggta agcgtacatg gctattggac gagtccgctc agtggggcgc
     1621 ggtatcccaa tcaatggacg ttgtccattc cttctgaaaa ggtcgagttg aacatttccc
     1681 ccagaatggc caatcaagaa ctcgtaacca ggcgtagtac cgatgtgacg tattgggaag
     1741 gagctgtgga tgtcactggg ctttggaagg gagaggaaat tcatggccag ggctatgttg
     1801 aactgaccgg atatgccgaa ccataccgcc ctggacaata agggggctgt tgaaaaaagc
     1861 cgccagcggc gttctcgtca tttttccgtg ctcacgtact ggaagtacgc tctgcgcgca
     1921 aaaatgactg cggccttgct ggacgaaccc ttcgaaaggc tcagggcatg cttttttgaa
     1981 ccgccccgat gcctcgtgca ccgtgcttgt gggtcaatat gctcttggaa attattatta
     2041 ttgaacactc ccataaggtg tgagctcttg ccatttaata atgaaggatg gttctaagat
     2101 gttccgcgct cattggattt tcgttgtttt tttgatgttc ggactggcgg cctgcgggca
     2161 acgaagcgat accattgtgt ccattgcctt acatcccacg aaacccaaca ttgtgtatgt
     2221 tgcgacggat gaagcggtct ataaatcttc ggatacggga gccacatgga ccagatttgc
     2281 cggcgaacta gcccggacgc gagtgatttg tcttgccctt gatccccagt tgtccgccac
     2341 cgtgtttgcc ggaacgatgg gcgatggaac ctataaaagc cccgatgggg gaagaacgtg
     2401 gcatccctat aactccggga ttcagaaagg cacgatctcg gctattgtca atcagattgt
     2461 atttaatcct ctgggaacgg agatggtata tgcgggaacg accgttgggg tgtttcgaag
     2521 tttggatggc ggtcgacatt ggaccgaacg gatgcaggga atgacggagg tgagttttgt
     2581 ggtgaccctg gccatcgatc ctcagcgtcc aaacgtcttg tatgcgggga ctaccggagg
     2641 cgtctatcgc accatcaatg ccacggaaag ttgggagaaa tcatcgacgg gaatggtggc
     2701 ttcagatgcc aaaatggcct caatggcgtt gggagtcaat ggaatcgtgg ttgacccaac
     2761 gaatagtgac gtggtgtatg cagggaccac ggacgggtta tataaatcaa cggaccaggc
     2821 cgagcattgg acaaaaatcg gaggaagtat tcaacacgcc tatattagtg ccatccaact
     2881 ggatcccact aacccttcca ttctgtatat ggcgacgagt gatcgggttc aaaaaagtga
     2941 ggacggagga gagacctggc aaccgaaaat taagggattg gaggcagcca gtattcgtag
     3001 tcttcagatg agccccatgg atccacgcat tctgtacgtt ggcaccaatg gaggagggct
     3061 gtaccgaagc atggatgccg gagagtcctg gatccgatta cctctggttc ctgcctcaag
     3121 tgagtagtcg ccaggattag atgctttttc ccctaccgtg ctcggatgcg tattcgaggg
     3181 cgtcttatcg ctctctccag gattcaggaa gcagaaacga gaggtccatc atgactttgg
     3241 ctccataggt tgggccgaag tgtgaggttg gtgctccatc cttcgtattg gaccagtggc
     3301 gagtccaact ggtggtaatc agatctaatc cgatggtttc acgggatgcc attgaaccgc
     3361 tccccttccc cattaaattc agtaaagcga aatatggaga atccggtagc aaagaccggt
     3421 tcctgatcgg ggaagttcag cgctccccaa tgcatacctg gtgcggaaat ttcggccctg
     3481 gccggttgtg gagatatcca ccaactcaaa caggtgatca ggatgatgac caaagtcaaa
     3541 gatgagtgaa atcggcaagc tatagtcaag gagatgaggc gttcaatttc agcgaggggt
     3601 agttggatga tttccagaac aacggcaatg tcttggagca tctgccctat agtgggggat
     3661 taccctattg gcaggcttac gcatgagatg tccggacttt acgagatata cccaatatgg
     3721 gatgaatcag tgaggaggga ggaaatcaga aatcgagagc ctcgtttact ttttcgacca
     3781 gttcacggaa attacagggt ttgtcaataa tcgcataggc gccaagtccc atggctttgt
     3841 gcttgtgtat ttcgtcaatg actccgctga gcaggattac ccgaggggtt ctgccgttca
     3901 aggtccggtt gactcgttcc aacagagtca gtccactcat aataggcatg tgatagtcag
     3961 caaggacgag atcgacatgg ttttcttcca accatgcgag tgcagccgcg ccgttttccg
     4021 actcagcgca ttcaattccc gaggattcta atgctccccg cacagttcgg cggacagcag
     4081 gttcatcatc cacaatcagc acatgttttc tgagagaggg agaccgtttg ttcaccacga
     4141 tgtgttcctc tgaaacacca taagaacatt caactttttc tcaggatgag ttggtcggtt
     4201 gttttgggaa agtttattct atttttgcat acgtaatgtg acttggggaa taccctcacc
     4261 atatatcttg tgtagatttt tatcaactac acaatgatta aatattcggc aaaatgatga
     4321 aggtgtagtg aatgatgctg gatcacaaat aatcaataac ttacgcacag gattgtccac
     4381 agttttggtg gatgggataa gttgaaataa ttggttaaaa agcgggagag ttacacttgg
     4441 gttttttgaa ggcaggatcc ggacaattgc gatttgactg attcataaat tttacttcct
     4501 ggcagaagtt ctatcccagg tatcccggac gcaatcgcct gctggacagc taaacgggcg
     4561 actctcatgc gtgggattgt ctgagagggt agcgcttctt gaagagcgat gaaagtcaca
     4621 ggaggaatct tgacagaaaa gagatccccg tcaggtgagg aaaagataaa gcattcgatg
     4681 tttgaagacc cgacacgata ggtcaattta aatgtgatgt tgtggggctg gagggagtca
     4741 tcatcagagg gaaattgctg aaagg
//
LOCUS       DAORAQ010000192         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2572_length_4748_cov_81.157255, whole genome shotgun sequence.
ACCESSION   DAORAQ010000192 DAORAQ010000000
VERSION     DAORAQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2572_length_4748_cov_81.157255"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..747)
                     /locus_tag="PKM72_12430"
     CDS             complement(130..747)
                     /locus_tag="PKM72_12430"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNP61641.1"
                     /translation="MNPSHIRFALLTQDPDLKSRVQGPDLQNTITVLDDCSSLEAAMR
                     SQQFTGVILDESGKKFFPALSALNGQLNLSKTFIYAGPLPAWSTMNQIRQVMGDQPSE
                     EAAIDPKDVSLASYVEKKLGDFVRAMNVGSGKNLYPTLMRAVERPLIELALRETHGNQ
                     IKAARLLGLNRNTLRKKITEFQISVSQVKESFAGKRKKPESDSAG"
     gene            complement(842..2329)
                     /locus_tag="PKM72_12435"
     CDS             complement(842..2329)
                     /locus_tag="PKM72_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HNP61642.1"
                     /translation="MSQSGPGSQTAIPTHQFGLFAGPLLAGLMFVLLPESLPESARIL
                     ATILTWVVVMWITEPIPLPITALLGCGLCVVAGLGTMQSVFSAFAHPIIFLFIGSFFI
                     SEALSVHGLDRRFGNWLLSRKCVGSNPIRIMMAMGLAVAGLSMWISNTAATAVMLPIA
                     LGVLRALRQGYPNMGTFDTGFLLCLAYGAGIGGVATLIGTAPNLIGVGLLAQQAHITI
                     SFDQWMLIGLPLACVMLLVMFTLLYWLHPPPSSSSFHEESPILKISAPLPWSLGEQYT
                     FAVFVLAVMLWILPALLSIWFEGDHVVLQWVRAHLPKELVPIFASGLLFLLPLNFRQG
                     KFTLTWKEAANIHWGTILLFGGGIAFGELMVETELAKAIGQGMVNMFGIQSIWGLTGV
                     AILTALVLTELASNTAATSMIVPVLIAIAHTANFSPVPPVLGACMAASLAFVLPVSTP
                     PNAIVYGTGRIPVLRMVQAGLLLDVIGGMLIWGTLRMLGPLFGID"
     gene            2530..3027
                     /gene="rfaE2"
                     /locus_tag="PKM72_12440"
     CDS             2530..3027
                     /gene="rfaE2"
                     /locus_tag="PKM72_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNP61643.1"
                     /translation="MHSKIHTLDSLLQRITIHQQAGESIVFTNGCFDLLHIGHTRYLA
                     EAKELGDRLVVGVNSDSSVRQLGKGTNRPIQSDAQRAEVIAALGCVDYVILFDEPDPL
                     NLIKAIQPNVLVKGGDWNPERIVGKDFIEERGGFVRTIPLVSDVSTTAIIERILTTQG
                     INPSS"
     gene            3068..>4748
                     /locus_tag="PKM72_12445"
     CDS             3068..>4748
                     /locus_tag="PKM72_12445"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNP61644.1"
                     /translation="MNIPFVFHHFANQLRTVVPAIPERTPPPCLLSTQQACLALAMTL
                     FTLPEKKTAGIEGHTHLIITPTQEQAEELYEDLEFFFSFIRRDHETLALFPPWATIPY
                     NPALPSHSVICQRVRSLHRLSRGESTVMVTSLPAILHKLLPPEVFAEACFSLKIGETC
                     EREVLLRSLIRLGYERGSLAEVPGEFSVRGGIVDIFSTAQDHPVRVEFLGDTVESIRT
                     FDPSTQESITHLKETWVLPTREFIPPDSGQPDSAIEHIQASIEWDLPRYYPKLVTLFD
                     YIHTPVTVSFYRPVDLDAAAVQFWNALLETWEQLQPGPGGVAAHADPDQLYEMWETIK
                     TYTSMCPTVWWDSVAPDTNTMPCVPVSLQAQSPSSVGVGIRGLPFKETLSKLDQLRAD
                     SEIFFVARSPGQVERLLSLLHDHGFPANKWEYPFPQPAPDVRAPFYCLEGDLSAGFLS
                     PDGHIAFVTEEELFGKGIRHRPHTKSPTAKFFSSLEDLKEGDLVVHLQHGIGRYLGLR
                     RLEVQGYTSDYLLLQFGGTDTLYVPLDRLNHIHRYRGAEQRSPRLDRLGGTA"
BASE COUNT         1268 a         1255 c         1138 g         1087 t
ORIGIN      
        1 gccgacctct accgtgtcaa ggtagcgctc tccccctgag ctacgcgcct atattcttac
       61 cggccatatg ctagactgca ttggtggtct tgtcaagaaa tggttatttt tcaaggggaa
      121 aaggaaaggt tatccagcag agtccgattc cggcttcttg cgttttccgg caaaagattc
      181 ctttacctga ctaacggaaa tttgaaactc tgtaattttt tttctgagtg tattccgatt
      241 gaggcccaga agcctggctg ctttaatctg attgccatgc gtttccctta aagcgagctc
      301 aatcaagggc cgttcaacag ctctcattaa agtgggatag agatttttcc ctgaaccgac
      361 attcatcgct cgaacaaaat cccccaactt tttttccaca taactggcca ggctcacatc
      421 tttcggatca atagccgcct cttccgaggg ttgatctccc atgacctgcc ggatttgatt
      481 catggtactc caggcgggca ggggtccggc ataaataaat gttttcgaca aattcaattg
      541 gccattcaat gccgacaagg caggaaaaaa tttcttcccg gactcatcca gaatcactcc
      601 ggtaaattgt tgagaacgca tcgccgcttc caaggaagaa cagtcatcca acaccgtaat
      661 ggtgttctga agatctggac cttgaacccg ggacttgaga tcgggatctt gggttagcag
      721 agcaaaccgt atgtgagaag ggttcaaaga cacgttattt tagtcaggtg aaatgcaaac
      781 gaggattatg tcggaacatt atttaaaaat caattgaggt ctatcttctg ggttgggaag
      841 atcaatcaat tccaaaaagt ggacccaaca tacgtaatgt accccaaatc aacatgcctc
      901 ctatcacatc caacaacaat cccgcttgca ccattcggag aaccggaatc ctgcccgttc
      961 catagacaat ggcattcggg ggggtagaaa caggcagcac aaatgccagg cttgcagcca
     1021 tacaggcacc cagcactgga gggacaggag aaaaattggc cgtgtgggca atggcgatga
     1081 gcaccggcac aatcatgctg gtcgctgcgg tatttgatgc aagttccgtg agaaccaggg
     1141 ctgtaagaat agccacaccc gttaaacccc agatcgactg aatgccaaac atattcacca
     1201 taccctgccc gatcgctttc gccaattcag tttcgaccat caactcgcca aaggcgatac
     1261 ctcccccgaa taacaggatg gttccccaat gaatattggc ggcttctttc caggttaagg
     1321 tgaatttccc ctgtcgaaaa ttcaagggca gaagaaacaa aagcccggag gcaaaaatag
     1381 gcaccaactc tttcggcaaa tgagcacgaa cccattgcag gaccacatga tcaccttcaa
     1441 accatattga gaggagggcc gggaggatcc acaacatgac cgccaacaca aagaccgcaa
     1501 atgtatattg ctccccaagt gaccaaggca gtggtgccga aatcttgaga ataggagatt
     1561 cttcgtgaaa agacgaggaa gaagggggag gatgcaacca atataaaaga gtgaacatca
     1621 cgagcagcat cacacaggcc aacggcaacc caatcagcat ccattggtca aacgaaatgg
     1681 taatatgggc ctgctgggct aataagccca ccccgattaa attcggcgcg gtgccaataa
     1741 gagtggccac tcccccaata ccagcgccat aagccaggca aagcaaaaac ccggtgtcaa
     1801 aggtccccat attaggataa ccctgtcgaa gtgcacgtaa tactccgagc gcaatgggta
     1861 acatgaccgc cgttgccgca gtattgctaa tccacatgga caaaccggct actgccaaac
     1921 ccatggccat catgatacga atagggttag agcccacgca cttacgtgac aaaagccagt
     1981 tgccaaaccg gcggtccaac ccatgaacgg atagggcctc agaaataaaa aaactgccaa
     2041 taaacaggaa gatgatcggg tgagcaaagg cagaaaacac ggattgcatg gtccccaatc
     2101 cagcgacgac acacaatcca cagcccagca aagcggtaat gggcagagga ataggctcgg
     2161 taatccacat caccaccacc caggtcaaaa ttgttgccag aattctcgca gattcgggca
     2221 atgattcggg aagcagcaca aacatcaacc cggcaaggag ggggccggca aaaagaccaa
     2281 attgatgcgt aggaatggcg gtttgtgaac ctggaccaga ttggctcatg cagctcttgg
     2341 gatggacgcg atcaaactta ggaagtccgg taggcctaga gacctcgacc gtatatcctc
     2401 ttttccgtgt atgcctaaat ggccttgtcg ggtaacctct ctaggatttg gaagtatgca
     2461 gcaggtatca tacagaagtc aggatatgtg agatagagac ctcatgaaag gattcaacga
     2521 tttatcatta tgcactcaaa aattcatacc ctcgatagtc tattacaacg aattaccatt
     2581 catcagcagg cgggagaatc cattgtcttt acgaatgggt gttttgactt gcttcatata
     2641 ggacatacgc gatatctggc agaagccaag gagttgggag atcgattagt ggtcggagtg
     2701 aatagcgaca gttcagttcg tcaactgggt aaggggacaa atcggccaat ccaaagcgat
     2761 gctcagcgag cagaggtcat tgccgcatta gggtgcgtag attacgtgat actgtttgat
     2821 gagccggatc ccttgaatct cattaaagcg atccaaccca atgtccttgt gaaaggtgga
     2881 gattggaatc cggaacgaat agtcggaaaa gattttattg aggaacgggg aggatttgtg
     2941 agaacaattc cacttgtctc agatgtttct accaccgcga ttattgaacg gattctcacc
     3001 acgcagggaa taaacccatc ctcctagtca gcacgcagat aaacattttc gtggtgtatt
     3061 tcagatcatg aacatccctt tcgtcttcca tcattttgca aaccagctgc gaacggtcgt
     3121 tccagcgatc ccggaacgta cacccccgcc ctgtttactg agcacacaac aggcctgcct
     3181 cgccctggcc atgaccctgt tcacccttcc ggagaaaaag acggccggca ttgagggcca
     3241 cacccacctc atcatcacgc caacgcaaga acaagccgaa gaattatatg aagacctgga
     3301 atttttcttc tcgttcatca ggcgagatca cgagacactg gcattattcc caccatgggc
     3361 caccattccc tataatcctg cactgccgtc ccacagcgtc atctgccagc gcgttcgatc
     3421 tctccaccgg ttgtcccggg gtgagagcac cgtcatggtg acctcactac ccgccatatt
     3481 gcataaactg cttccgccgg aggtcttcgc tgaagcatgt ttttccctca agatcggcga
     3541 gacgtgcgag cgtgaggtgc ttcttcgttc attaatccgg ttagggtatg aacgtggatc
     3601 gttggctgaa gtccccggtg aattcagcgt acgtggcggt atcgtggata ttttctccac
     3661 agctcaggac catcccgtcc gtgttgaatt tcttggggat acggtcgaat ccatcaggac
     3721 gtttgatccc tcaactcaag aatccatcac ccatctcaaa gaaacctggg tcctacccac
     3781 tcgggagttc attcctccag attccggcca accggatagc gctatcgaac acattcaggc
     3841 aagcattgaa tgggatcttc cccggtatta tcccaaactt gtcacattgt ttgattacat
     3901 tcacacgccc gtcaccgtct ctttctaccg cccggttgat ctggatgctg cggcagtgca
     3961 attttggaat gcgttactcg aaacatggga acagctccag cccggcccgg ggggcgtcgc
     4021 tgcccatgct gaccccgatc aactctatga gatgtgggag accattaaaa cctataccag
     4081 catgtgtccg acggtttggt gggatagcgt ggctcctgat accaacacca tgccgtgtgt
     4141 gcctgtttcc ttacaggctc agtctcccag cagcgtcggg gtaggcattc gcggacttcc
     4201 ctttaaggaa accctttcca aactggacca attgcgagcg gactccgaga ttttttttgt
     4261 tgctcgcagt ccaggacagg tggaacgcct gctgtcctta ttgcatgacc acggcttccc
     4321 agccaacaag tgggaatacc ccttccctca gccagcccca gatgttcgcg ccccatttta
     4381 ctgtcttgaa ggagacctct cagcagggtt cctttcacct gatggacata tcgcgttcgt
     4441 caccgaggaa gaattatttg ggaaaggcat ccggcaccgt ccccacacca aatcacctac
     4501 tgccaaattt ttctcgtcgt tggaggatct aaaagaaggc gatctcgtcg tccatctcca
     4561 acatgggatt ggccggtatc tgggtttgcg ccgtttggag gtgcagggct ataccagtga
     4621 ttatttgctg ttgcaattcg gtggaaccga caccctgtat gttcccctgg accgcctgaa
     4681 ccacatccac cggtatcggg gagcggaaca acggtctccc agattggatc gcctgggagg
     4741 cacggctt
//
LOCUS       DAORAQ010000193         4718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2593_length_4718_cov_65.215526, whole genome shotgun sequence.
ACCESSION   DAORAQ010000193 DAORAQ010000000
VERSION     DAORAQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2593_length_4718_cov_65.215526"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1054
                     /locus_tag="PKM72_12450"
     CDS             110..1054
                     /locus_tag="PKM72_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61645.1"
                     /translation="MMELDVWNNWMSMALLTPALWAVSCIIDVCFVGERIFRFPSDGP
                     IISGLFCVLPFFVLATEVGGWEPVTLRTSWPALLAGACYFLHLYFVFRALFTMNDASC
                     SETFNTLSVLFVPVMAFVLLGERLDPIYYFAIVLALGGIFVLIRYHLSGVHRHAVVLL
                     TVAVLCISLTMVLQAWVFEQMGYWNGVVLFSLGTFLTALIVGGVQRRRRQRILGLCWR
                     FGGIFLVAELLQLAAVLASQRATAIGPSVSIVAVVECSLPLFVMVFSAGLLLSRYRKS
                     LSPAICDTLRLQMTSSPVKLTSVTLILIAILIVQITVI"
     gene            1088..1276
                     /locus_tag="PKM72_12455"
     CDS             1088..1276
                     /locus_tag="PKM72_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61646.1"
                     /translation="MDTCTFGEHLTIDGYGGEVAFLNDRYLVEHCLLDLPQLLGMHPL
                     IAPVVRWADANDHKDPGG"
     gene            1489..3363
                     /locus_tag="PKM72_12460"
     CDS             1489..3363
                     /locus_tag="PKM72_12460"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNP61647.1"
                     /translation="MAGAVTLDVCPSACTYSAIQAAVDVAASGDVIDIAPGTYVEVVQ
                     VNIPNLSFVGAGSASTIVDGPLSAFVFNASAGGASLSGVTLHSQVTGACIQNYADLVV
                     RDSVLENCSNDLNGGGIYTSGDLVLERVTVLASHLHYSQINPIEGRGGGLFVDGNPIS
                     GGPTVRIYDSAFGANEALYGGGIYVGTGAKVYIDGTTFSDNSAHDSTGLLPYTGRGGA
                     IRTFGELHVTNSTFFYNWADLFGGAIAVNCGSVPVYIATSVLFENFTNGETFTAGGLP
                     SGGGLYVESGGEVNVFGTEFYGNRSGYGGGIGVYNLLQQGTVNLVQGSLAYNTADGDG
                     GGLWVYGGNVTVNTSTVSLNLAKGNGGGAYGAYGSGIIDFKSSTIVDNLAYGTGGGLN
                     ANSMSLRNSILARNNASMANATGSTDDCNQSFDSRDHNLVGTVDGCGVLNLLPNDLSG
                     TDTALLDPQLGPLTDSDGGIEAGTYTHVHIPLTGSPLVDSGLRCANLDQNGNRRPVDG
                     GTGTRQCDRGAEELNAGCGSPVPTTALEPLDSGSNVALQPLFVWEPKAGANTYNVKLA
                     TQPPPFGTSIVAEVSGITATHWKPEVTLLSNQVYYWQVRAFSLCGGTKKSEVFSFTTS
                     "
     gene            4222..>4718
                     /locus_tag="PKM72_12465"
     CDS             4222..>4718
                     /locus_tag="PKM72_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61648.1"
                     /translation="MRRTILFSACLVFSMGSVASQVNAQEICRTAGFWGSHAGEEKSG
                     STNISEEVIRAWLSTVNSYDQTATDKCTYEMDGTVICNFEPPGQAGSPKACRDAKKND
                     ITLLNNFPIGYEGIDICGQIISVDPNGTDQIPPVLEALCVSPGGDSKLQLGRQLTAAA
                     LNCLL"
BASE COUNT         1054 a         1068 c         1270 g         1326 t
ORIGIN      
        1 tgtcagagta cgaatggttc tatccctcat tcttcatgaa cctatttggt aacgattttg
       61 acagatcaca tagagaacag ccactgatga tagtaacgga aagtagggag tgatggaact
      121 tgatgtgtgg aacaactgga tgtctatggc gttgttgacg ccagctctct gggcagtgtc
      181 ctgcattatt gatgtgtgtt ttgtggggga gcgtatattc cgttttcctt cagatggtcc
      241 cattatctct ggcctcttct gtgtccttcc cttcttcgtg ctcgctaccg aagttggggg
      301 ttgggagcct gtgacattgc ggacaagctg gccggcactg ttggccggtg cctgttactt
      361 cctccatctc tattttgtat tcagagccct tttcactatg aacgatgcct cctgctctga
      421 aacctttaat acgctcagtg tgttatttgt ccctgtgatg gcttttgtcc tgttaggtga
      481 gcgcttggac cctatctatt attttgcaat cgtgctggcg ctgggtggaa tttttgtgct
      541 gattaggtat cacctctcag gggtgcatcg gcatgccgtc gttctgttga ccgttgcggt
      601 gctgtgtata tcgctgacga tggtgctgca ggcctgggtc ttcgaacaaa tgggctattg
      661 gaatggggtc gtgctctttt ctctcggtac attccttact gcgttgattg tcggcggagt
      721 acaacgcagg cggcgtcaac gcattctcgg gttgtgctgg cgtttcgggg ggatctttct
      781 tgtggctgaa ctccttcaat tggcagctgt tttggcatcg caacgggcca cggctattgg
      841 gccttcggtt tcgatcgtcg cagtggttga atgtagtctc ccgctatttg tgatggtgtt
      901 cagtgctggg ctgctgcttt ctcgatatcg gaaatccttg agccccgcta tttgcgacac
      961 attgcgactc cagatgacgt caagccctgt caaattgaca tcggtcacac tcatactgat
     1021 cgccattttg atcgtgcaaa taacggttat ttaaggagcc agaattttta aaaaggagtg
     1081 aaatgtcatg gacacctgta cgtttgggga acatttgaca attgatggat atggtggcga
     1141 ggttgcgttt ttaaatgacc gttatctggt ggaacactgt cttctcgatc tgccccaatt
     1201 actcggcatg cacccattga tcgcgccagt ggttcggtgg gcggacgcga atgatcacaa
     1261 agatcctggt ggatgaagtg gatttgtgct gattgctgaa agccacatca gtattcatac
     1321 ctttccccac cggcattttc tgatcgacaa caaaaggaat cagaaatgaa tttttacatg
     1381 ccccatccaa ttcgacgaca aattcatctt tttccagtgt ttttgaagtt ccaacgactc
     1441 ctctgtcttc tatttctgag caatgtatgt attgctttgg gaggtaccat ggctggggca
     1501 gtaaccttgg atgtgtgccc gtctgcctgc acctattcgg cgattcaagc ggccgtggat
     1561 gttgccgcaa gcggagatgt catcgatatc gcgccaggta cctatgtgga agttgttcaa
     1621 gtgaatatac ccaatctcag cttcgtcggg gctggtagtg cgagcactat tgttgacggt
     1681 ccgctcagtg cgtttgtatt caatgccagc gcaggaggtg caagccttag cggggtgact
     1741 cttcatagcc aagtaacagg agcctgtatt caaaactatg cggacttggt cgtccgggat
     1801 agcgttctgg agaattgttc gaatgatctg aacggtgggg gcatttacac cagtggtgat
     1861 ttggtccttg agcgggtcac cgtgcttgcc agtcatctgc attattctca aattaacccg
     1921 attgaagggc gtggtggagg tctatttgtc gatgggaacc cgatcagtgg tgggccgacg
     1981 gtgcgaattt atgacagtgc gtttggagcc aacgaagcct tgtatggtgg gggaatctat
     2041 gtcgggactg gagcaaaggt ctatattgat gggaccacct tttcggataa tagcgctcat
     2101 gacagtacgg gacttcttcc atacaccgga cggggaggcg cgattcgcac gtttggtgaa
     2161 cttcatgtga ccaatagtac atttttttat aattgggcag atctatttgg tggggccatt
     2221 gccgtcaatt gcggctcagt cccggtttac atcgcgactt ccgtactttt tgaaaacttc
     2281 accaatgggg agacgttcac tgccgggggg ctcccatctg gaggaggact ctacgtggaa
     2341 tcaggcggag aagtgaatgt gtttggcacc gaattctatg gaaatcgatc cggttatggt
     2401 ggaggaatcg gagtctacaa cctgttgcag cagggaacgg tcaacctcgt gcaaggcagt
     2461 ctggcctata acacagccga tggagatggg ggcggactgt gggtctatgg aggcaatgtg
     2521 accgtcaaca cctccacggt ctccttgaac cttgctaagg gtaacggggg aggcgcctac
     2581 ggcgcctacg gatcggggat tatcgatttt aagagttcaa cgatcgtcga taatcttgca
     2641 tacggaactg gcggggggct gaatgccaat agtatgagtt tacgaaactc tattcttgca
     2701 cgaaacaatg cctcaatggc aaatgcaacc gggtcaaccg atgattgtaa tcaatcattc
     2761 gattctcgtg atcataatct agttggaaca gtcgacggat gcggggtcct caaccttctt
     2821 ccgaatgatc tgagtgggac tgataccgca cttttggatc cccaactcgg tccactgacc
     2881 gatagtgacg gcgggattga agccgggaca tacactcacg ttcatatccc gttaaccggg
     2941 agtccgcttg tggattcggg ccttcggtgt gcgaatttgg atcaaaatgg gaatcgtcga
     3001 ccggtggatg gtggaaccgg caccagacaa tgcgaccgtg gagcggagga actaaatgcc
     3061 ggctgtggtt cgcctgtgcc gacgaccgct cttgaacccc ttgacagtgg aagtaatgtg
     3121 gcactccaac ccttgtttgt ctgggaacct aaggctggtg cgaacacata caatgtcaaa
     3181 ctcgcgactc aacctcctcc atttgggaca agcattgttg ccgaggtttc cggtatcacc
     3241 gcgactcatt ggaagccaga ggtgacgttg ctttctaacc aagtttatta ctggcaggtc
     3301 cgggccttca gcctttgtgg tggtacaaaa aagagtgaag ttttcagttt tacgacttcc
     3361 tagttgcacc aaatgtttgt ggaattttag ttccttgtcc gtaggtgtgt cagaatatag
     3421 tgagaaaagt cctgctgttt gcccactcaa acagcgtggt acggtgtaca ccattaaatg
     3481 gtaatccccg atggccttgt ctagacggaa attcttcctc gctgcagcgc tttgggcgtg
     3541 ggtgtttcgc catttttttg agatgccctt aaaaaagtgc gtataatttg gtcccccttg
     3601 gaaggaacgc cctcaatgtg ttcaacatgc aattggccag cggcagacgt gttgaaatga
     3661 tgaggctcct ttctcccaat tacaatcgat ctgttttgag gaatgccgat gaaacccaaa
     3721 gattggatct cactcatcga agcgggcaat tctttagacg ggaatgtgga agtgtggctt
     3781 aagaatgtgt tcgaccaggc ttccccgctg tttgatcgcg gatggtggcc tgagatgtgg
     3841 acttaccgac atgtttcacc attatgcgtc caatgtcttt tttttgataa aagttgcagg
     3901 caagagagct cattaccatg caaccgatag gatgttgcta gatctctttt ctttaatttc
     3961 cctccagtgc tcctgcctgg gttaagttgt cctcccacag gccgataagg cagagggcat
     4021 tgttctccgg gtattccccc aaaatcttat agtcagcggt cttcacaggt ttcaatactc
     4081 ccaagaacgc atgggatggt ttttcgactc catgccacta ttgagggatt ttaactgaag
     4141 ggtttgaaat ctcatcagtt tggggaaatg taacaatcct cacgaaggaa acactcactt
     4201 ttttctcagg ggggtttaac aatgagaaga acgatattgt tttcggcttg tcttgttttt
     4261 agcatgggaa gtgttgccag ccaggtaaat gctcaagaga tttgccggac ggcaggattc
     4321 tggggctctc atgcaggcga ggagaaatcg ggtagtacta acatatctga agaagtcatc
     4381 cgggcctggt tgtccaccgt caattcctat gatcaaacgg cgacggacaa gtgtacctat
     4441 gaaatggatg gcacggtaat ttgtaatttt gaaccgccag gccaggcggg aagtccgaaa
     4501 gcctgtcgag atgcgaagaa gaatgacatc accctgttga ataatttccc tatcggatat
     4561 gagggaatcg atatttgtgg acaaattatt tccgtagacc ccaatggcac cgaccaaatt
     4621 cctcccgttc tcgaagcgtt atgcgtttcc ccaggcgggg attcgaaact tcagctcggt
     4681 cgccaattaa ccgccgcagc gctgaattgc ctgcttag
//
LOCUS       DAORAQ010000194         4697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2609_length_4697_cov_62.372684, whole genome shotgun sequence.
ACCESSION   DAORAQ010000194 DAORAQ010000000
VERSION     DAORAQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_4697_cov_62.372684"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKM72_12470"
     CDS             complement(<1..224)
                     /locus_tag="PKM72_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61649.1"
                     /translation="MNAAEQQVNEVRKLKRSEAHGWLIHLFQVMVVVGLLLPTFALPT
                     VWAEDVPDVIGLSQGAAESAIMGAGLTVGT"
     gene            643..1320
                     /locus_tag="PKM72_12475"
     CDS             643..1320
                     /locus_tag="PKM72_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HNP61650.1"
                     /translation="MEKNTEGKPTTEVQDDPKARELLQAAFAKTARWQPDFKGFQSDL
                     RVNVNGKETKGTVTVKGPRDVTVVIDDAELQTWAQNQIGMIAVHRGPRTFQESDGKHT
                     LTLEGDDQHPLGQRITIHGDGMGSWYRVKDNRITQINRNMPQAAFTINVEDSAVTQDH
                     HHLTTRYTVYYFSPKDRSLKNVDSFSDTHIRVGNSDLPATRRIISYENGEIVVRSLTF
                     ENHKMLS"
     gene            complement(1536..2270)
                     /locus_tag="PKM72_12480"
     CDS             complement(1536..2270)
                     /locus_tag="PKM72_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNP61651.1"
                     /translation="MTMYEKRFVGPLILLLLLVFSAPSMGMTPKEQNLPTPLGYVSDY
                     ANLLDEEWHKQIRSVCKDLESSSGIEMLVVTLPTISPLAHAQEYASRLYEAWRIGTAQ
                     QERGILLLVAVKERQAVVVVGKNLLPILTPQQLDEISLKHLRPMFRSGEYGINIYRSA
                     VSLSTLAARAPIPKDKPTPQRSAAFWLNIGVVVMMLYALWRFTRPERRHPFPRWKNGE
                     YWGTGQGGFGGNFGGFGGGTGGQGLR"
     gene            complement(2267..3031)
                     /locus_tag="PKM72_12485"
     CDS             complement(2267..3031)
                     /locus_tag="PKM72_12485"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNP61652.1"
                     /translation="MPTTTLQSLELPEDLREILEQYLTQLQKDWGADLEGVLLYGSAA
                     RGDFIAGRSNINFLVLVRKLSVSGLQRAGQLHRQWGKHHIVAPLVMTQEDLSRSCYLF
                     PLEYFQMQEHHVLLVGKDPFGAGQIDGTQLGWQCEQELLANVFRVRQRFVEGEGRAEA
                     IQALLILSITAVLPCVRGILRALGHPSKRKDVQTLECLPSALQFEPTVLLEMLQMKRG
                     INSPGSLEWPKVYDRYLQTLEDLCKRVQAVRQNRGL"
     gene            complement(3090..4364)
                     /locus_tag="PKM72_12490"
     CDS             complement(3090..4364)
                     /locus_tag="PKM72_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNP61653.1"
                     /translation="MDLQTQVVIVGAGGGGAVLGLLLARKGIANIVLEQASGPPQGLR
                     GEILQPNGQQILQGLGLLDQLPVHAYKPVRFFHFRQSGGTRLCTIDYGMLPPPFNRAL
                     VMWPHVVHHTILDALRRENPEGLRYGATFQSLIRNGKGIVGVKADIGGSPVTIGSQLV
                     VGADGPFSEVRGALGIPTALHRYLDSYLVAMLESPGPLEEAQYYVGKKSILGIFPAAG
                     QKVYAFYMVSSDSLNQVKADGVSALREKWKTIAPDLAPVFDSLLDWNQTTYMGTGRVR
                     AKTWVQDGAVVMGDAAHGMNPHASQGRMQAMVDAVTLAEVLEECHRTGNWSTSALKRF
                     ETQRRPQVTMLQRLADEEVFFWNTGNPLLGMLRDRVFSTMDKNPRLQYQVLTATAGLR
                     TTSPFGWVDRFQAAGLLPDPRAHQIPDHARST"
     gene            complement(4492..>4697)
                     /locus_tag="PKM72_12495"
     CDS             complement(4492..>4697)
                     /locus_tag="PKM72_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61654.1"
                     /translation="TTVYVRTATLDPEKGVGFLQPLQGRTHQIKSMLSSAQKMYLRQW
                     LSSSYPPAWEESDDHFQSIFSEP"
BASE COUNT         1249 a         1278 c         1131 g         1039 t
ORIGIN      
        1 atggttccta ccgtcaggcc cgctcccata atggccgact ccgccgcccc ctgactcaac
       61 ccaatcacat cggggacatc ttcggcccat actgttggca acgcgaaagt gggtaacaac
      121 agacccacca caaccatgac ctggaaaaga tgtatcagcc aaccatgggc ttcacttcgt
      181 ttgagttttc ggacttcatt tacctgttgc tctgcggcgt tcatgcgcaa tgcctcttgg
      241 gggtggaagc aagcgaaaca tctctgtaaa agggatgaaa aacacggaca tctatgcccg
      301 taattctctc ctcagataaa aaattatgga tgcagcctag ccgaacacca ccttcttttc
      361 aacgtacctt tatctcccac ttttttagcc ttttatatgc atccggggaa agagtgaaac
      421 ctttagcaaa gatgagattc tgacagaggg tttttggaaa atgtaattgt tgccacgaca
      481 aatttcccga tacaaccaag gaggctgagg attgcaagag tcctccggat ctcactccat
      541 ctaaacggtt gtcgacctct tctgagatcc ttataatgat cctcagtgtt cgctcaatca
      601 gcctggtcac ataccgacat cacaggaaaa ggagtcttcg ccatggaaaa gaatacagaa
      661 ggaaaaccaa ccaccgaggt ccaggatgat ccaaaggccc gcgaactgct acaagcggcc
      721 tttgccaaaa cggcccgatg gcaacctgac ttcaagggct ttcagtccga ccttcgggtt
      781 aatgtcaacg ggaaagaaac caaagggact gtgaccgtga aaggaccacg ggatgtgacg
      841 gtggtcatcg atgatgcgga actacaaacc tgggctcaaa accaaattgg aatgattgcg
      901 gtgcaccggg ggcccagaac ctttcaggaa tcagacggaa aacataccct gactttagag
      961 ggagatgacc agcacccttt gggacaacgg atcaccattc atggagatgg catgggttcc
     1021 tggtacaggg tgaaggacaa ccgcataacg cagatcaacc gaaacatgcc acaagcggct
     1081 tttaccatca acgtcgaaga tagtgcagtc acccaggatc accatcacct caccacccga
     1141 tatacggtct attatttttc accaaaagac cggtcactga aaaatgtcga cagtttttca
     1201 gatacacaca tcagggtcgg caactcagat cttccggcca cccgtcgcat tatttcttat
     1261 gaaaatggtg aaattgtcgt gcgatcgtta acgtttgaaa atcataaaat gttgtcataa
     1321 accatatcct cccaggccgg cataaagttg gcctgggaat atcggatttc tagtcgtgat
     1381 cgaccttccc aacaatttca tttccagaag tctcaaaaaa tccatggaga gggttggagc
     1441 atcacatagc cggatttcga gaaaacggtc ccaaaaatcc acccacataa ttcatgatga
     1501 gaaaagggat ggtcgatagg ttcagtccag ccccttcaac gtaagccctg gccacccgtt
     1561 cctccgccaa atccaccaaa gtttccacca aatcccccct ggcccgttcc ccaatactct
     1621 ccatttttcc aacggggaaa gggatgacgc cgttcgggac gggtgaaccg ccataacgca
     1681 tacaacatca tcaccactac cccgatgttc aaccaaaaag cggctgatcg ctgaggggtg
     1741 ggtttgtcct tcggaatagg agcccgcgcg gccaacgtcg aaagggatac ggcagatctg
     1801 tagatattga tcccatactc tccggaacga aacatggggc gcaaatgctt gagggaaatc
     1861 tcatccaatt gttgtggagt gaggatcggc aaaaggttct ttccaaccac caccacggcc
     1921 tggcgttctt tgacagctac cagcagcaga atgccacgct cctgttgcgc cgtaccaatc
     1981 cgccaggctt catagaggcg tgaggcatat tcctgggcat gggcaagagg gctaatggtt
     2041 gggagagtca ccaccagcat ttcaatgccg gaggaggatt caaggtcttt gcacactgat
     2101 cgaatttgtt tgtgccattc ctcatcaagc aggttggcat agtcactcac atatcccagt
     2161 ggggttggga gattttgttc cttgggagtc attcccatcg agggtgcaga gaaaaccagc
     2221 aggagtagca ggatgagcgg accgacaaac cgcttttcat acatggtcat agccccctat
     2281 tctgacgaac cgcctgaacc cgtttgcaca agtcctccaa agtctgaaga tatcgatcat
     2341 acacctttgg ccattcaagg gaacccggac tattgatgcc tcgcttcatc tgaagcattt
     2401 cgaggaggac cgtgggttca aactgcaagg cagaagggag gcattcaaga gtctgcacat
     2461 cttttctctt tgaggggtgt cctaaggccc gaagaattcc tcgcacacag gggagaacgg
     2521 cagtaataga tagaatcagc aaagcctgga tggcctccgc tcgtccttct ccctcgacaa
     2581 accgttgccg cacccgaaac acattggcca ggagttcctg ctcacattgc catcctaact
     2641 gtgtcccatc aatttgcccg gcaccgaatg ggtctttccc taccagaagc acatggtgct
     2701 cttgcatttg aaaatattcc agggggaaca gataacatga tcgggacaag tcctcctgtg
     2761 tcatcaccag tggtgcgacg atatggtgct taccccattg gcgatgcaat tgccctgcac
     2821 gctgaagccc gctcactgaa agtttccgga ccagcaccaa aaaattgata ttggaccggc
     2881 ccgcaatgaa atctcccctg gccgcacttc catacaggag aactccttct aaatcagcac
     2941 cccaatcctt ctgaagctgc gttaaatatt gttcaaggat ttcccgaagg tcctcgggaa
     3001 gttcaagcga ctgcaacgtt gtggttggca taggatacag accaaatatt ggaaaaatga
     3061 tgagtgacga agatggactc cgaagttaat tatgtagacc gggcatgatc gggaatctga
     3121 tgggcccggg gatcgggtaa caatcctgca gcttgaaacc gatccaccca tccaaatggt
     3181 gatgtggtcc gcagccctgc tgtggccgtc aaaacttgat attgcaaacg cggattttta
     3241 tccatagtag agaacacacg gtctcgcagc attcctagga gaggatttcc ggtattccag
     3301 aaaaagacct cttcatcagc caggcgctgc aacatggtga cctgtgggcg ccgttgtgtt
     3361 tcaaaccgct taagagcgga cgtcgaccaa tttccggtcc ggtgacattc ctcaaggact
     3421 tccgcgaggg tgacggcatc gaccatcgcc tgcatccgtc cctgggaggc atgtggattc
     3481 atcccatgcg cggcatcacc catgacgacg gccccatcct gaacccaggt tttcgcccgg
     3541 accctcccgg ttcccatata ggtcgtctga ttccaatcca gtaaggagtc aaaaaccggg
     3601 gcaagatccg gagcaatggt tttccatttt tcccgaaggg ccgacacccc atcggccttg
     3661 acctgattca gagaatcgga cgaaaccata taaaaggcat agaccttttg accggccgca
     3721 ggaaaaattc ccagaataga ttttttgccc acataatact gggcttcctc aagtggacct
     3781 ggtgattcca acatggccac aagataactg tcgagatatc gatgaagcgc cgtgggaatc
     3841 ccaagggctc ctcgaacctc agaaaacggt ccgtctgccc caaccaccaa ttgagatcca
     3901 atagtaaccg ggcttccccc tatgtccgct ttgacgccta cgatgccttt gccattgcgg
     3961 ataagggact gaaaggttgc cccataccga agtccctctg gattctcccg gcgcagagcg
     4021 tcgagaatgg tgtgatgaac gacatgaggc cacatcacca gcgcccgatt aaaaggcggc
     4081 ggcaacatcc catagtcaat ggtacagagc cgcgttcctc cactttgacg gaaatgaaaa
     4141 aatctcaccg gtttgtaggc atgaacaggc agttggtcta agaggccgag cccctgaaga
     4201 atttgctgtc cattgggttg aagaatctcc ccccgcaagc cctgcggagg ccccgaagcc
     4261 tgttccagaa caatgttggc aatccctttt cgggctaaga gaaggcccag gacagctccc
     4321 ccgccaccag caccgacgat cacaacctgt gtctgtaggt ccacgactaa tacctttgaa
     4381 tcccttcaca aaaaaattcg acctgttccc gcgccaccag ggtgagcgag aaaccgattt
     4441 atcaacagtc cgattgcaca aatcgatcgt cctccagcgt ggaggaggag gtcagggttc
     4501 tgaaaaaatt gactgaaagt ggtcatcaga ctcttcccag gctgggggat aggaactcga
     4561 caaccactgc cgaagataca ttttctgcgc ggaggacaac atggatttta tttggtgggt
     4621 ccgtccttga agcggctgaa gaaatccaac ccctttctcg ggatctaacg tggccgttcg
     4681 aacatacacc gtcgtat
//
LOCUS       DAORAQ010000195         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2624_length_4674_cov_69.599264, whole genome shotgun sequence.
ACCESSION   DAORAQ010000195 DAORAQ010000000
VERSION     DAORAQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2624_length_4674_cov_69.599264"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..514
                     /locus_tag="PKM72_12500"
     CDS             236..514
                     /locus_tag="PKM72_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61655.1"
                     /translation="MLRVTPRIDETTITLQLEGRLAGPTVIEAERCWQVTCAENPGRS
                     HRLDLRGVTYLDQEGKSFLNRVFQQGASFLSSGCLTRAYVEEITRTGK"
     gene            552..1424
                     /locus_tag="PKM72_12505"
     CDS             552..1424
                     /locus_tag="PKM72_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNP61656.1"
                     /translation="MTQMKSISGIYKSGSTHMVGDGFPVRNIFPSQNLTEEISPFLLL
                     DYAGPASFPPTNQRLGVGEHPHRGFETVTIVYQGKVAHRDSAGNGGTIGPGDVQWMTA
                     ASGVVHEELHEQAWAKEGGTLQMIQLWVNLPKSLKMGAPRYQTVLNEDIPQSNLGGQG
                     GVLRVIAGEYEGLKGPAKTFTPVHLYDLRLVAGHLGELSLPVGYNTGLFVLSGKLVLN
                     KSHEVGEVEMALCDPQGTKVNFEAMEEARVLVLSGEPIPEPVARMGPFVMNTEEELVQ
                     AVNDFRAGRMGRLE"
     gene            1653..2909
                     /locus_tag="PKM72_12510"
     CDS             1653..2909
                     /locus_tag="PKM72_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNP61657.1"
                     /translation="MNAAILRPVSVYIGNGRAVIRRSVGLLLILLAGCGQGDTSPPAP
                     PPPTVEVVTVTTQDLPDEPEFIGQTEAFRPVEIRSQVTGIIKKVFFTEGRNVKQGDRL
                     YLIDPVPFKAAYLSAKARVTQAEARLVQAKQDFARVKPLLKEQAVSQKDVDDAVAEGL
                     AAKASLEAAHGDLVKSRFDLSNTLIVAPIGGRISKSRFYEGRLVSAQTDLMTTIDQLD
                     PMYVNMSVPETYLLRRRRELADHKVERPDLFQLRGVMTFADGSVYPHEGKLDFADVAI
                     RSETGTLQGRFAFPNPEADLSPGQSYLYPGHFVRIRLKGYIRTDAILIPQRAVQQGPK
                     GTFVNVIGPDDTIEVREVDATGWRGGDWLIEEGLQPGERIVVEGFHRIQPGMQVNPVP
                     FRNGEATSDTPGHENPTPPEATQENS"
     gene            2906..>4674
                     /locus_tag="PKM72_12515"
     CDS             2906..>4674
                     /locus_tag="PKM72_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNP61658.1"
                     /translation="MSSHFFIDRPIFATVISIVIVVVGLVSLQVLPIAQFPEITPPVV
                     QIEADYPGASAEVVAEAVARPIEVQLPGIDNLLYYDSSSTNDGHMTIRLTFEIGTDVD
                     IAQVQTQNRQRLAEPQLPPEVVRQGITVKKVSPDLLGVVALSSSDPRQDTVFLSNFAI
                     LRIVDNIKRLPGVGDALIFGGQNYSMRLILDPIRMAQMDVTPTDIANVVREQNRDFPA
                     GRIGREPAPSGTELTIPVITQGRMSEAKEFEDMIVRAYPDGSMVRLGDVAKVELGAQS
                     YDLEGRWNGKPNVFMLTFLSPGANALETMKRIKDEMKSLTNIFPAGVSYDIPYDTTRF
                     IEVSIQQVVKTLGEALILVILVVYLFLQSWRATLIPAVAVPVSLIGTFAGMVALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENVARHIQNGHPPKEAAKLAMTEVTGPVIALVVVL
                     AAVFLPVAFLGGITGELYQQFAITITLSVVLSGIVALTLSPALCALILKPDQERETGF
                     WKKFNDGFNWTQNRYVGTVGSILKHAVLSLAVFGVLLFVIGGLFQVLPSSFLPDEDQG
                     YFISVVQLPDGASKQRTIDVIEQV"
BASE COUNT         1073 a         1113 c         1361 g         1127 t
ORIGIN      
        1 gcggtggaat ggacgtttga cctgaccttt aaaggcggaa atcgggtgaa gatgcagcag
       61 gtggccgtgc aaacctggaa ggatggaaaa attattcgtg aggactttta tcacgggtaa
      121 aaggattctt atgtgttgaa ccatgtgtgc ccacgacagg tttacgctca aaaacaatgg
      181 cagcctaaga gatcccgggc tcctgttctc aactgttttg ataaggaatg aagttatgct
      241 cagagtcacc ccccgcatag atgagacgac aatcaccctc cagttggagg ggcgattggc
      301 cggaccgaca gtcatcgaag ctgagcggtg ttggcaggtg acatgcgcgg agaatccggg
      361 gcgatcccat cgactggatt tgcggggggt gacatatttg gatcaggaag ggaaatcgtt
      421 tttgaaccgg gtctttcagc agggcgcgag ttttctctct tccggttgtt tgacccgggc
      481 ctacgttgag gaaatcacgc gaaccgggaa ataatctgct gaaaaagcag tattgaggaa
      541 aggaggatgc catgacgcag atgaaatcga tttcaggaat ctacaaatcg ggatctactc
      601 atatggtagg ggacgggttt ccggtacgga atatctttcc aagtcagaat ctgaccgaag
      661 agatcagtcc ctttctgctc ttggattatg cgggtccggc atcgttccct ccgacgaatc
      721 aacggctggg tgtcggtgaa catcctcatc gtgggtttga gaccgtgacc atcgtctatc
      781 agggaaaagt cgcgcatcgg gattcagccg gaaacggagg aactatcgga ccgggcgatg
      841 tgcaatggat gacggcggct tcgggtgtgg tgcatgaaga acttcatgaa caagcctggg
      901 ccaaggaggg tggcacgttg cagatgattc aactctgggt caatcttccc aagtcattaa
      961 aaatgggcgc gccccgctac caaacggtcc tcaatgaaga cattccgcaa tccaatcttg
     1021 ggggacaagg cggtgtgctc cgtgtgatcg ccggcgaata tgagggactc aaagggccgg
     1081 ccaagacgtt cacgcctgtg cacctgtatg atctgagatt ggttgccgga catctgggtg
     1141 agctcagtct tccggtcgga tataatacgg gcctgttcgt ccttagtggg aagttggtct
     1201 tgaataaatc tcacgaagtc ggggaggtcg aaatggctct ctgcgacccg cagggaacga
     1261 aggtaaattt tgaggccatg gaggaggcaa gagtgttggt gctgagtgga gaaccgattc
     1321 cggaaccggt cgcccgcatg ggtccttttg tcatgaatac tgaggaagag ctggtgcagg
     1381 ctgtgaacga ttttcgtgcg gggagaatgg ggcgacttga gtaagccgat aatcgagctt
     1441 cttgatggaa ggaaagcgtg aaaaattttg ttcggctgta ataatagtgg tgaaccttcg
     1501 tctacacatt tacagaagat gatgccctgc aatgaagctg aaggaaagac aacccttatg
     1561 gatgagccaa tcattcagag gacagcacaa attgaaaatt ccgacttaaa tcttgtgaat
     1621 ggaatagaac ggaattggtg ggaaccaacc agatgaatgc agccatactc agacccgttt
     1681 cagtatacat tggcaatggg cgcgccgtga ttcgtaggag tgttggatta ctcctgattc
     1741 ttcttgcagg atgtgggcag ggagatacct ctcctccggc tcctccaccg ccgacggtgg
     1801 aagtggtgac ggtcaccacc caggatctcc ccgatgaacc cgaattcatc ggacaaaccg
     1861 aggcctttcg cccggtggag atcaggtcac aggtaacggg catcattaag aaggtgtttt
     1921 tcaccgaagg caggaatgtc aagcaaggtg accggctgta cctcattgac cccgtgccct
     1981 ttaaggcggc ctatctgagt gccaaagcta gggtgacgca agcggaggct cgactggtac
     2041 aagcgaagca ggattttgct cgagtcaaac ctcttctgaa agagcaagcg gtcagtcaaa
     2101 aggacgtgga cgatgccgtg gcggaaggat tagccgccaa agcctccctg gaagcggcgc
     2161 atggggatct ggtcaagtcc aggtttgacc tgagtaatac cctgatcgtc gctccgatcg
     2221 gtggacgtat ttcgaaaagc cggttttatg aaggacgtct ggtgtccgcc cagacagacc
     2281 tgatgaccac catcgatcag ctcgatccca tgtatgtcaa tatgtccgtt ccggaaacgt
     2341 atttgttgcg gcggcggcgt gagttggcgg accacaaagt cgagaggccc gatctctttc
     2401 aattacgcgg agtcatgacg ttcgctgatg gcagtgtcta tccccatgaa ggcaaattgg
     2461 attttgccga tgtggccatc cggtcggaga cggggacctt acagggccgg tttgccttcc
     2521 ccaatcctga ggctgatctt tctcccggtc aatcctattt gtatcccgga cattttgtcc
     2581 gcatccggct gaaggggtat atccggaccg atgcgatttt gattcctcaa cgtgcggttc
     2641 aacagggtcc taaaggcaca tttgtgaatg tgatcggacc cgacgataca atcgaagtgc
     2701 gcgaagtgga cgcgaccggt tggcgtggcg gcgattggct cattgaagaa ggccttcaac
     2761 ctggagagcg aatcgtcgtc gaagggtttc atcggattca accgggtatg caggtgaatc
     2821 cagtgccgtt ccggaatggt gaggccacat cggatacccc gggtcatgag aatccgacac
     2881 ctcccgaagc cacacaggag aactcatgag ttctcatttt tttattgacc gtcctatttt
     2941 tgccacggtc atttccattg tgattgtggt ggtgggactg gtgtctctcc aggttcttcc
     3001 catcgcccaa tttccggaga tcactccgcc cgtagtccaa attgaagccg actatccggg
     3061 tgccagtgca gaagtcgtgg ccgaagccgt ggcgaggccg attgaggtgc aactccccgg
     3121 gatcgataat ctcctgtact acgattcttc cagcaccaac gacggccata tgaccatccg
     3181 attgaccttt gaaatcggga cggatgtcga tatcgcacag gtccaaactc aaaaccgtca
     3241 acgtttggcc gagccacagc ttccgccgga agttgtccga cagggtatca ctgtcaaaaa
     3301 ggtgtctccg gatctactcg gggtggtggc gttgagttcc agtgatcctc gtcaggatac
     3361 ggtctttctt tctaattttg cgattctgcg gattgtggat aacatcaaac ggcttcccgg
     3421 agtgggggat gcgttgatct ttggcggaca gaactactcc atgcgtctta ttttggaccc
     3481 cattcgtatg gctcaaatgg atgtgacgcc cactgatatt gcgaatgtgg ttcgtgagca
     3541 gaaccgggat tttccggctg gccggatcgg acgcgagcct gccccgagtg gaactgagct
     3601 gaccattccc gtgatcacgc aaggacggat gagcgaggcc aaagaattcg aggacatgat
     3661 tgtccgggcg tatccggatg gttccatggt gcgtctcggc gacgtggcta aggttgagtt
     3721 gggggcacaa tcctatgacc tcgagggacg gtggaatggc aaacccaatg tctttatgct
     3781 gacattcctc tccccgggag ccaatgccct tgaaaccatg aaacgaatca aggacgagat
     3841 gaagagtctc acgaatattt ttccggctgg agtctcatac gacatcccgt acgatacaac
     3901 ccgattcatt gaggtttcta ttcaacaagt ggtgaagacc ctgggagaag cattgatcct
     3961 ggttattttg gtggtctacc tctttcttca aagctggcgc gcaacattaa ttccagccgt
     4021 ggctgtcccc gtttcattga tcggcacgtt tgcgggtatg gtcgccctcg gattttcgat
     4081 taataccctg actttgttcg gcatggtctt ggccatcggg attgtggtgg atgatgcgat
     4141 cgtggtggtg gaaaatgtgg cccggcacat tcagaacgga catcccccaa aggaagccgc
     4201 gaagctggcc atgacggaag tgaccggccc ggtcattgcg ctcgtcgtgg tgttggcggc
     4261 agtctttctg cccgtggcgt ttctgggagg tattaccggc gaactctatc agcaatttgc
     4321 aatcaccatt acgctgtccg tggtcctttc cgggatcgtg gccttgacac tcagtccggc
     4381 cctctgtgcg ttgattctca agcccgatca agaacgggag accgggtttt ggaaaaaatt
     4441 taatgacgga tttaattgga cgcagaatcg atatgtgggg acggttggct cgattcttaa
     4501 acacgcggtg ctctcgctgg cggtctttgg ggttctgctt tttgtcatcg gtggactgtt
     4561 tcaagttctg ccgtccagtt ttttgccgga tgaagatcag ggatatttta tttcggtcgt
     4621 gcaattgccg gacggggcat ccaagcagcg aaccattgac gtgattgaac aggt
//
LOCUS       DAORAQ010000196         4639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2643_length_4639_cov_63.488438, whole genome shotgun sequence.
ACCESSION   DAORAQ010000196 DAORAQ010000000
VERSION     DAORAQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_4639_cov_63.488438"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKM72_12520"
     CDS             complement(<1..763)
                     /locus_tag="PKM72_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61659.1"
                     /translation="MRNRQSLTMSFLVGCMLIAAPLVAQAGGTIKGKVTFAGEVPPPK
                     EFAFSKFPNVEFCKKNESKSADGETRLLKEVEVDGSKGLKNAVVAVTDIQDKAFLGKA
                     DKEAPQLVEAELCEFKPYTGVAVSKGLFKVVNNDADPSDPKSAEGVLHNPHSFDQLGA
                     KSSTEFNIGLAKKGDSLEKSVKLKMSKKGSVLRLQCDQHEFMQGWYLPVTNAHYSVAG
                     ADGTFEIKDVPAGKHKVKAWHPIAGEVEADVDVKDG"
     gene            complement(1065..2933)
                     /locus_tag="PKM72_12525"
     CDS             complement(1065..2933)
                     /locus_tag="PKM72_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNP61660.1"
                     /translation="MLAGFGAWLLVSPVHAEIRPHSESINHPIIQWHEWGNEAFERAQ
                     AEDKLIILDLTAIWCHACHVMDQTTYANPRIVQLLNTKFIPVRVDTDQRPDLDARYRA
                     GGWPTTNVLLPTGEILFQANALGSEEMETMLLEVQSIYVADKGELLKQASHLWERVNE
                     KVKNDSSRGNELQASMAKQSVAMMRAQFDAVNGGFRDAPKFFEPEAIQMVFAYGFFEE
                     DPELLKIGLDTLKRQVRLLDPVWGGFYRYAEQADWSQPHFEKMLSIQALNLRNYVEAF
                     QLTGDLQFKRIALALIEYVSRFLTDSQTGLLYESQDADVLGTEGEAFMPGAEYYSLNE
                     SKRLAVGIPYVDQRVFTGSNALMAWAYLHASLVLEKVEIGNMALQILSRLYEKRFDME
                     MGLAHVETGGVPSLYGLLSDHVLFGQALLEAFKVTGQSQYLQNAEALADVSRRLLHDS
                     ENGGFFDHPQTSGKLGLLKFPAKPVKENLQAAIWYLDLFHLTGKQPYRFLAENALQAM
                     VTSREPLPLALSGLAIDQWFRIPIHIAVVGDFDDAMTKTLLVEGQRMFCPGKIVKSFD
                     SKEGPPKWGDIIFPYEERPVAFVCTDRMCSAPVFHAEDMKKSVADMVALLRDPVQK"
     gene            complement(3146..4489)
                     /locus_tag="PKM72_12530"
     CDS             complement(3146..4489)
                     /locus_tag="PKM72_12530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP61661.1"
                     /translation="MNRYNAMFFLGVLWIFSGSVWAEEAQEETVVQVQKAYEQGLFQQ
                     VVDLVKPLEKEFSSPSQVSRLYILALANLGKTPEAIETYDKEVGVTKREDEPLLRQMA
                     IASILPLRSDMREQMRGAAYTALKEIESDNAVEYLEKGLTDENGMVRTLVAEALGQRK
                     AGLRSKVFREALNDEAGLVRATVLGGLGQSGDVSLVPLIEPSLNDEQHLVQIAAARAL
                     YQLGHKQYWARLEQGAKSEEGYERGAAIRAFGELGDRRAIPILEKTISDSQPSIRAAA
                     IASLGKLKAPESLPTLKAMLFDRIPAVRSVAAFSMGYYEQHQVLTPLTRALADSNSGV
                     QAAAVASLLKSGAPYSVVRNTVQHLLNDQNPAIRSGAVKALGNGKGQDVLNALMLALN
                     DPLPRPRIGASRALGRIGDRTLLPTLKRTLRDTDEAVRVTAAAAIVRILDKEIGI"
BASE COUNT         1231 a         1263 c          970 g         1175 t
ORIGIN      
        1 ccccgtcctt cacatccaca tcggcttcca cttccccggc aataggatgc caggccttta
       61 ctttgtgctt accagcaggc acgtccttga tttcaaaggt tccgtcggca ccggccacac
      121 tataatgcgc attggtcacc ggcaaatacc agccttgcat aaattcatgt tggtcacatt
      181 gcaaccgcaa cacagacccc tttttcgaca ttttcaattt gacgcttttt tctaaagaat
      241 cacctttttt ggccagaccg atattgaact cggtgctgga cttggcacct aattgatcaa
      301 agctatgagg attgtgcaac acaccttccg ccgacttagg gtcgctggga tcggcatcat
      361 tattgacaac cttaaacagt cctttactta cggctactcc ggtatatggc ttaaactcac
      421 acaattcagc ctccaccaat tgcggagctt ccttgtcggc tttgcctaag aatgctttgt
      481 cttgaatatc cgtaaccgcc accaccgcat tctttaaacc cttgcttcca tctacttcca
      541 cttccttcag caatcgtgtc tctccatccg cactcttgct ctcatttttt ttgcaaaact
      601 ccacattggg aaattttgag aaagcaaatt cttttggtgg gggtacttct ccggcaaacg
      661 tgactttccc tttaatagtt ccccccgcct gggcgactaa tggggcagca ataagcatgc
      721 agcccactaa aaaagacata gttaaagact gacggttcct catgattctt cctcctagtt
      781 aagaatgaaa ttgagttgac gtgggaaaac aacctaactt gatgagatca acccctttta
      841 ccccggccag gtataaaaaa gctgatcttg aaatggatcg tccgacagca cccacttccg
      901 tatatatcag gaataaaaaa aacgtgtcaa gaaacccaac aagaactagg tcctttgtgc
      961 taaccattag gccttttagt tcgcggaaac atgaaaagtt taaacagaca atattttccc
     1021 taaaaatcaa tggaatggta accgggcatc tgaacacact cgcatcattt ttgtacaggg
     1081 tccctgagta gggccaccat gtcggccaca ctctttttca tgtcctctgc atgaaagacc
     1141 ggagccgaac acatccggtc agtacagaca aacgcgacag gtcgttcctc atagggaaaa
     1201 ataatgtcac cccatttcgg cggtccctcc ttggagtcaa aacttttcac aatttttcca
     1261 gggcaaaaca ttcgctgacc ttccaccaat agggttttgg tcatggcatc gtcaaaatct
     1321 cctaccaccg caatatgaat agggatccga aaccattgat cgatagccaa tccggacaat
     1381 gcgagaggga gtggctctcg ggacgtgacc atggcctgca aggcattctc agccagaaaa
     1441 cgatatggtt gcttccccgt cagatgaaaa agatcgaggt accaaatggc ggcttgaaga
     1501 ttttccttca ccggtttagc tgggaacttc agaagaccta atttaccaga cgtctgtgga
     1561 tgatcaaaaa atcccccatt ctccgaatcg tgcaataacc gccggcttac atcggcgagg
     1621 gcttcagcgt tttgtaaata ctgagattgc cctgtcactt taaaggcctc aagtaacgct
     1681 tgaccaaaga gcacatgatc agacaataaa ccgtaaaggc ttggaacccc tcccgtctca
     1741 acatgagcaa gccccatttc catgtcaaaa cgcttctcat acaatcggct caatatctga
     1801 agagccatgt tccctatttc gactttttcc aataccaatg aggcatgcaa ataggcccaa
     1861 gccattaatg cattactccc cgtaaaaact cgttggtcca catatgggat accgacggcc
     1921 aatcgtttgc tctcattgag agaatagtac tcggctccag gcatgaaagc ttccccttca
     1981 gttcccagaa cgtcggcatc ttggctttca tacaataacc ccgtttgcga atcggttaaa
     2041 aaccgcgaaa catattcaat gagcgccagg gcgatgcgtt taaattgaag atccccggtg
     2101 agttggaagg cttcaacata gttgcgaaga ttcagagctt gaatcgacaa catcttttca
     2161 aaatgcggtt gactccaatc tgcttgttcg gcatagcggt aaaatcctcc ccaaaccgga
     2221 tcaagtaagc gcacttgtct ttttaaggta tccaatccta tcttgagtag ctcaggatcc
     2281 tcctcaaaga agccataggc aaaaaccatt tggatggctt ccggttcgaa gaatttaggg
     2341 gcatcccgaa accctccatt gacagcgtca aattgagctc tcatcatagc cacactttgc
     2401 tttgccatag acgcctgaag ctcattccct ctggaagaat cgttcttcac cttctcgttt
     2461 acccgctccc ataaatgaga ggcctgcttg agcaactcac ctttgtcggc gacgtaaata
     2521 gactgcacct caaggagcat ggtttccatt tcctcagaac ccaaggcgtt cgcctgaaaa
     2581 agaatttctc cggtcggcag aaggacgttg gtcgtcggcc accctcccgc tcgataacga
     2641 gcatccaaat ctgggcgctg atccgtatcg acccgaacag gaataaattt ggtgttgagc
     2701 aactgtacga tacgagggtt agcgtaagtc gtctgatcca tcacatggca ggcatgacac
     2761 catatggcgg ttagatccaa aataattaat ttgtcttcgg cttgcgcacg ttcgaacgct
     2821 tcatttcccc attcatgcca ttgaataatt ggatggttga tagactcact atgaggccgt
     2881 atttcagcat gaacgggact gacgagaagc catgcaccga atccggccag catccataat
     2941 ctgcaacctc cctggatacg gcataagtca ttcagcacct cagtcaccga gacgaatgcg
     3001 aacttgatga tcgtgcgcca gaattggccg aaaggaatgc agctcccaat gaaccgcaca
     3061 agttggagga acatgtgaac accctttatc tggtcgtacc tgtccatctt caggaagacc
     3121 gcttataggg caaaaaccaa accggttaaa taccgatctc cttatccaaa atcctcacaa
     3181 tggcagcggc ggcggtcacc cgcaccgctt catcagtatc tcgaagtgtg cgcttcaaag
     3241 tcggtaagag agtgcggtcg ccaatacgac ccagtgcgcg ggaagcccca attctgggac
     3301 gaggcaacgg atcattgaga gccaacatga gagcattgag gacatcctga cctttcccat
     3361 ttccaagcgc cttgactgca ccggaccgta tggccggatt ttggtcattg agaagatgct
     3421 ggacggtatt cctgactact gaataaggcg ctcctgactt tagcaatgac gccacggccg
     3481 ccgcctgcac gccggaattg gaatcggcca aggccctggt taacggtgtt aacacttgat
     3541 gttgttcgta ataccccatg ctaaaagccg ccacactccg aactgcagga attcgatcaa
     3601 acaacattgc tttcaatgtc ggtagggatt caggcgcctt caatttccct agggatgcaa
     3661 tagctgcggc acgtatcgaa ggctgtgagt cagaaatagt tttttccaat attggtattg
     3721 cccgacgatc ccctaattcg ccaaaggcac ggatagctgc cccccgttca tacccttcct
     3781 cgcttttcgc accttgctcc aaacgagccc aatattgctt gtgacctaac tgatataatg
     3841 ctcttgctgc ggcaatttgg acaagatgct gctcatcgtt caacgatggt tcaatcaatg
     3901 gcaccagact cacgtctcca gattgtccaa gtccacccag aacggttgcc cgtaccaaac
     3961 cggcctcgtc gttcaatgcc tcacgaaaca ccttggaacg cagtccggct tttctttgcc
     4021 ctaaagcttc tgccaccaat gttctcacca tgccgttttc atcagttaag cctttttcca
     4081 aatattccac ggcattatca gattcgattt cctttaacgc ggtataggcg gcccctcgca
     4141 tttgttcccg catatcggaa cggagaggca atatactggc aatggccatc tgccgtagca
     4201 aaggctcatc ttcccgcttg gttacgccaa cctctttatc ataggtttca atggcttcag
     4261 gagtttttcc caggttagcc aaggcaagaa tgtacaggcg agaaacttgc gagggggaag
     4321 agaactcttt ttctaatggc ttaacgaggt caacgacctg ctgaaaaaga ccctgctcat
     4381 aggctttttg cacctgaaca accgtttcct cctgtgcctc ttccgcccaa accgaacctg
     4441 agaaaatcca caggactccc agaaaaaaca ttgcattata acgattcatc cttttttatt
     4501 tacccccatc tgctagccac atcatatgtc taccccctcg gaatcggggc ttcctgagcc
     4561 tcaataattc gatagcctaa tggtgcttca aagtaataat ctggttgcga agaaagcacc
     4621 acatgatgat attccacaa
//
LOCUS       DAORAQ010000197         4617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2663_length_4617_cov_66.423718, whole genome shotgun sequence.
ACCESSION   DAORAQ010000197 DAORAQ010000000
VERSION     DAORAQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2663_length_4617_cov_66.423718"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKM72_12535"
     CDS             complement(<1..619)
                     /locus_tag="PKM72_12535"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNP61662.1"
                     /translation="MAPHSHLTLLLSLLVLLTTTLGASRSPAPKRTTTVVPVAAHPQE
                     ENIPFYPSAPRLLATAPKGLYLVVDTAQNRVSLRKGNRILYSAVASTGSGARLQDPRN
                     PGNGWVFDTPRGVFTISSKIKNPAWNKPDWAFIEEGQPIPTKPKDRIETGVLGDYALG
                     FGNGYFIHGTLYTRTLGTNVTHGCIRLGDEPLEYVFHHVPLGTTLI"
     gene            complement(633..1868)
                     /locus_tag="PKM72_12540"
     CDS             complement(633..1868)
                     /locus_tag="PKM72_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNP61663.1"
                     /translation="MRENTLITPPTCFESAWRRRWIITLMLVLLPALGYAWWGHTGYP
                     ETFPPLVEDLDRQARANGAEALFPDRYQQFHHQVIKLRSQWRREANHWWTNGDEATFN
                     QQYQQLVEEGSLLIQASRQKVSALRTEVKELLQPEQAHLTRLRELSHLFDLEDDMFAL
                     SLAEGLLQESILRLEQGQYRQARAAGEQALEHLRRVEPHVLAQMNRYRNDGQITLWEQ
                     WVTDTIQRSTGTAGTAVIVLKAPRRLLVYQRGRIIADYPARVGFSGLADKLYEGDGAT
                     PEGQFHVIHKKDGSGTNYYKALLLDYPTVAHQRRFQDAQANGLVPADRSIGSLIEIHG
                     EGPNNENTTSGCIALENSTMDDVFGRTQVGTPVTIVGALNQDNEVVNLVRRMEAHIHE
                     RNERWHTLPTFAASFTSTE"
     gene            complement(1868..2536)
                     /locus_tag="PKM72_12545"
     CDS             complement(1868..2536)
                     /locus_tag="PKM72_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61664.1"
                     /translation="MTERLQLNMAVWKSTSERSPRQVNLLHYTHALFLLWLLLAGCAE
                     PPLSQWNAATQALEDARMSEAQQYAIEKFTEAETAYQQVKQNLDQQGDRIPLFRNYDP
                     AITILSEVFNDATQAKMEAIANKRESKANAEVALAFAKQHLQDVRALLAEVSTPASDH
                     GKLDQLLQAFQDTETLLAEIDSIMAQEHFIDVMTTSHSVESFATRLQARIISLKRLPA
                     KQQA"
     gene            complement(3222..4001)
                     /locus_tag="PKM72_12550"
     CDS             complement(3222..4001)
                     /locus_tag="PKM72_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNP61665.1"
                     /translation="MNTSGPHESYNLDIQTPAGNLAASVSVPTGFIPLTDILPLMRSL
                     GEQAQQLEIDNTTQTGDTISCQKGCAACCRMMIPVAPPEAFALLSVVEALPSPKKERL
                     LERFQAAQEILREAGLEDGLQRLAFSEHQGSDEELEPLNRAYYALRMPCPFLENEICS
                     IYEQRPSACRELLVTSPAELCQDVIQNPVNLIPSPFRIGTVLSKLWADCYQGPIRLIP
                     LPFALDWAKAHESQTTRTWAGPELLSRALDAATQYLQRRSS"
     gene            4437..>4617
                     /locus_tag="PKM72_12555"
     CDS             4437..>4617
                     /locus_tag="PKM72_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61666.1"
                     /translation="MLERVMDRSVFRTKRLLSHPRSEDSHELRLKRDLTVWHLVALGI
                     GAIIGTGVFVLIGTAT"
BASE COUNT         1006 a         1067 c         1368 g         1176 t
ORIGIN      
        1 tgatcagggt ggtgccaagg ggcacgtggt gaaagacata ttcaagcggt tcatcgccaa
       61 gtcggatgca gccatgggtg acgttggtgc ccagcgttcg cgtgtacagc gtgccatgga
      121 tgaaatagcc attgccgaag ccgagggcat agtcgccgag caccccggtt tcaatccggt
      181 ctttgggttt agttggaatg ggttgccctt cttcaatgaa ggcccagtct ggtttgttcc
      241 aggccggatt tttaatttta ctggaaatgg taaagacccc acgcggagta tcgaataccc
      301 atccgttgcc gggattgcga ggatcttgta accgcgctcc actgccggtt gaggctacgg
      361 cactgtagag gattcgattg ccctttcgaa gggacacccg attttgagct gtatcgacca
      421 cgaggtacag gcccttgggt gccgtggcga ggaggcgggg agcggaagga taaaacggga
      481 tgttttcttc ctgggggtgc gccgccaccg ggaccactgt tgtagtgcgt ttgggggcgg
      541 gggagcgtga ggccccaagt gtcgttgtca gcaggacgag taggctcagc agaagtgtca
      601 ggtgtgagtg gggggccatg aagaatgaca ttctattctg tgctggtgaa tgaggccgca
      661 aatgtgggga gggtgtgcca gcgttcattt cgctcgtgaa tatgcgcttc catccggcgt
      721 actaagttca cgacctcatt gtcttggttc agtgccccga cgatggtgac aggcgttcca
      781 acttgtgtcc gaccaaatac gtcatccatg gttgaattct ccaaggcaat acagccgctg
      841 gtggtatttt cgttattcgg tccttcgcca tgaatttcaa tcagactacc gatggatcga
      901 tccgctggaa ccaggccatt ggcttgggca tcctggaagc gcctttggtg ggcaacggtg
      961 gggtaatcta ataataaggc cttataataa ttggtccccg atccgtcttt tttgtgtatc
     1021 acatgaaact gaccttccgg cgtggcgccg tctccctcgt agagcttatc tgccaatcct
     1081 gaaaatccca cgcgtgccgg atagtcggca atgatccggc cccgttggta gacgagtagg
     1141 cgacggggag ccttgagcac aatgaccgct gtgccggctg tcccggttga gcgttgaatg
     1201 gtgtccgtga cccattgttc ccagagggta atctggccat cgtttctgta gcgattcatc
     1261 tgagccagta cgtggggttc cacgcggcga agatgctcaa gggcctgttc tccggctgcc
     1321 cgtgcctgcc ggtattgtcc ctgctctaat cgcaagatac tttcttgtaa cagcccctct
     1381 gccagggaaa gcgcaaacat gtcgtcttcc agatcgaaca ggtgggacag ttcacgtaat
     1441 cgtgtgagat gagcttgttc gggttgaagg agttccttga cctccgtgcg aagcgctgac
     1501 actttttggc gcgaagcttg gatcagcaga gagccttctt ccaccagttg ttgatactgc
     1561 tggttgaagg tcgcttcatc gccgtttgtc caccagtgat tggcctccct ccgccattgg
     1621 gaacggagtt taatcacctg gtgatgaaat tgttggtaac gatcaggaaa taaggcctct
     1681 gcgccgttgg cccgggcttg tcggtccaga tcctcgacca gagggggaaa ggtctcggga
     1741 taccctgtgt gcccccacca ggcatacccc aaggctggaa gcaggaccag catgagcgtg
     1801 atgatccagc gccgacgcca ggccgactca aaacaggtgg gtggagtgat caaggtgttc
     1861 tctctcatca ggcctgttgc tttgcgggaa gccgtttgag agagataatc cgggcttgaa
     1921 gtcgggtggc aaaagattct acggaatggg aggtggtcat cacgtcgatg aaatgttcct
     1981 gggccataat ggagtcgatt tcggcgagga gcgtttcggt atcctgaaag gcttggagaa
     2041 gttggtccag cttcccgtga tcagatgcgg gagtggacac ttctgccagg agggcacgaa
     2101 cgtcttgaag atgctgtttg gcaaaggcga gagccacctc ggcattcgct ttactctctc
     2161 ttttattggc gatggcttcc attttggctt gggtcgcatc gttgaagact tctgacaata
     2221 tcgtgatcgc aggatcataa tttcgaaaga gcggtatccg atctccctgt tgatccagat
     2281 tttgcttgac ctgttgatat gccgtttctg cttcagtaaa tttctcaatc gcgtactgtt
     2341 gggcttccga cattctcgcg tcttcaaggg cttgcgtggc tgcattccat tgggagagag
     2401 gtggttctgc gcaaccggct aagagcagcc agagcagaaa aagtgcatgg gtgtagtgga
     2461 gaagattaac ctgtcgaggg ctgcgttcac tggtggattt ccatacagcc atgttgagtt
     2521 gaagtctttc tgtcacgatt attggagcat cctttgacat cgggaaggtc gttcttcatg
     2581 aagaggaaga ggggccgaaa cgttccggcc cctcttgtcg ttcgcccgca tcaaaacctg
     2641 aacaggtagg gccttttcct caatggtaaa aggtcgggag ttgcaggtcg tggggatcgt
     2701 caggatacct atttgccgaa tggctttttg ggcggatttt gctgtggttt agcagggctg
     2761 ggagcggcag tcggtttccc gattggcata ccctttttgg gatctttttg attggattgg
     2821 ctcacagtag cacctccttt gaaggttagg taagccgtca tggcttacta ctcggagata
     2881 taaaagcaaa ggtaatgcca gtttttgatc cgtggtgcag gggtaatttc cgtatttttt
     2941 gcggataagt cgatgaaatt tcgcgaagga acatgatgcg aagagacatc ctgtccagtg
     3001 caagaaatct tgtccaaaat ttctggaaat tctggtcccg attggtaggt tcgttttaaa
     3061 attttctcct gcggttccag ggagggtcgg caatcacttc tggctagtca tccgaggctt
     3121 gaccagccct taccattctg ttgcctacgc tggaggatac ctggtcggca cacacggagg
     3181 ccgttgaata cgtccaggaa actgaaggtg ttcaatgcct cttatgatga tcggcgttgt
     3241 aaatattgtg tggcggcatc taaggcccgg ctcagtagtt caggccccgc ccaggttcgg
     3301 gtggtttggc tttcatgagc cttggcccag tccagggcga aggggagggg aatgaggcga
     3361 atcgggcctt ggtaacaatc tgcccatagt ttgcttagga ccgtgccgat tcgaaacggg
     3421 gagggtatca gattgacggg gttctgtatc acgtcttggc aaagttccgc aggggacgtg
     3481 accagcaatt cccgacaggc ggagggccgc tgttcataga tggagcagat ttcgttttcc
     3541 agaaatggac agggcattcg aagcgcatag tacgcgcgat taagaggttc cagttcttca
     3601 tcggagcctt gatgttcgga aaaagccaat cgttgcaatc catcctccag gccggcctca
     3661 cgtaagattt cttgagccgc ctgaaatcgc tcaagcaaac gctccttttt tggagaaggg
     3721 agtgcctcga caaccgacag gagcgcaaag gcctccggtg gggcgacggg aatcatcatg
     3781 cgacagcagg cggcacagcc tttttggcag gaaatggtgt caccggtttg agtggtgttg
     3841 tcgatttcca actgttgagc ttgttctcca agggatcgca tgagggggag aatatcggta
     3901 aggggaatga atccagtcgg tacggaaacc gacgccgcca gatttccggc aggggtttga
     3961 atatccaggt tgtacgattc gtgaggtccg gaggtattca tgagaaaatc ttatgaaaga
     4021 acaatcaact aaacagatta cgttaataga cgtcattgcc cggccgtgac atgcactgga
     4081 ggggagccgg agattgaatt gtagaagaac ccgtatgttg aggaaaagct gatggcttgg
     4141 gttcacccga catgccgggg agggcatgat tttctgcatg gcgccattcc aagagaatca
     4201 tgggtcttac aagatacccg ggcttttacc ttgagagttt taattcttga ggaagaacga
     4261 attcaaattc aagtccttct gcttcaaatg ggcttctcgt atccaggggg ctgacatcag
     4321 gcacagttat agcatagtcg ggtcgtgctt agggagtgaa gatctctttt ttctaaaccg
     4381 gtcctcccct taaggcataa aaaccgtacc ttggaaagga aagtcgaacc accactatgc
     4441 ttgaacgtgt gatggatcgt tcagtatttc ggacgaaacg tttactgagc cacccgcgct
     4501 ccgaggattc ccatgaatta aggttaaaac gggatctgac tgtgtggcat ttagtcgcgc
     4561 ttgggattgg cgccattatt gggaccggag tgtttgtctt aataggaacg gccacgg
//
LOCUS       DAORAQ010000198         4608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2667_length_4608_cov_60.428948, whole genome shotgun sequence.
ACCESSION   DAORAQ010000198 DAORAQ010000000
VERSION     DAORAQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_4608_cov_60.428948"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(740..2062)
                     /locus_tag="PKM72_12560"
     CDS             complement(740..2062)
                     /locus_tag="PKM72_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNP61667.1"
                     /translation="MASRLYQWIDQRLKLKPLEQTLLNEPIPGGASWNYIFGSATLFL
                     FGLQALTGMFLMVYYVPATDHAYDTVQYIQHEVWGGWFVRGLHHWGASAVMMAIGLHM
                     LQVFFDGAYKRPRELMWIVGIILLAIMLGFGFTGYLLPWDQNAYWATQVGINMVGSVP
                     LIGDFLVKILRGGETLGALTISRFFAIHVAFLPAAIMFGIMLHLFILRRVGPAGPHDE
                     AKAKAGSETFYPRQVFMDAVVMLVVFGIVSLLAFSVEFPLADRADPSDHSFIPVPEWY
                     FLFFYQLLKYAPGAWGPLATWLLPTLFFTGLLLLPFLDRNPERQPSSRRVVLGAGLGF
                     LIIVFGLLSISLRDLYAVPKRDPSVVRGIALVEQFHCKTCHRIHGEGVNVAPDLSFVA
                     DRRPDRDWHLQHFKDPQSVSPGSFMPKFPLNDKQLNDLTNYILTLKSE"
     gene            complement(2065..2586)
                     /locus_tag="PKM72_12565"
     CDS             complement(2065..2586)
                     /locus_tag="PKM72_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNP61668.1"
                     /translation="MEDGLSTPVGTRRSFFVKTTVFISSLIGISLAVPLIGYIITPAF
                     QRRKKEWMSLGKADALPVGEPKAMDYTMTEKDGWQETQTTKGVWAVKQPNGEVTVFSP
                     ICPHLGCGYRWDQQDRLFKCPCHGSIYDLEGTVKAGPAPRPLDTLPSKIENGELLVQY
                     EEFKSGLAKKVEL"
     assembly_gap    3016..3115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3116..3642)
                     /locus_tag="PKM72_12570"
     CDS             complement(<3116..3642)
                     /locus_tag="PKM72_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNP61669.1"
                     /translation="MSQETEEIWSRRFFLKSSLAGMLILGSRLMFPESAAAHSLPEGR
                     LRLYNAHTDERLQVTYRNQSGRYDQEALKDINYLLRCHHSKKVCQMDIQLLEYVNQVE
                     KLVGQGKEIQIYSGYRSPSYNKLLIRLGRGAAPNSLHTVGQAMDFSIPGVRLSKIRRA
                     AVKLRLGGVGYYGRQG"
BASE COUNT         1197 a         1058 c         1138 g         1115 t
ORIGIN      
        1 ttaaagccat taatcagatg aaacgtgaag aaaaagaggc cccgcctgcg gatccgacaa
       61 cgaaagaatg caaattttgt cagatgacga ttccgataaa ggctctacgg tgtggtcatt
      121 gcacctcgga gttggccaaa actgcctaat ggttcatgag gaaatgccct gtcatcaagt
      181 atagcctaca gaaatgcgga tgatgtgtgt tgaacgggtt gtctggaaat acatttatga
      241 accgggtgct aatcggtagg aaatagcagt tcctcataca gggcgggcca gaaagacggc
      301 attctcagta agcggaatgc cgtcttcgct atgccaaaaa ttcatattcc aataatcaaa
      361 ctttccctat atcgggttga acgaattttt ccagaatctg tggaattggg cttcaggtgg
      421 aattacgttt tcccctttgt tacggttgaa ctctgtgctg gctctataga cttttcgtat
      481 tctctccaga aattagccat aaattctttt gtcgaacagt ccgtccccag aaaatacatc
      541 tgattgggct agtggtgtat ttcctgcttc tctttggttg gctgtgggga attctcctat
      601 ccccatgatc agccttaatc tgatgccggt gtcgtgtctg gccatccaca atggtgagcg
      661 caacattgtg agcaggtagc atcaaattga tgtctgctct caacgatgct caccgatttc
      721 cacaggggag gaagaagcgt tattcactct tgagagtcag tatgtaattg gtgaggtcgt
      781 ttagctgttt gtcgtttaga ggaaattttg gcatgaagga tcctggggaa acggattgcg
      841 gatccttaaa atgttgaaga tgccaatccc ggtccggtcg tcgatcggcg acaaaagaaa
      901 ggtctggggc aacattcaca ccttccccgt gaatgcgatg acaggttttg caatggaatt
      961 gttccactaa ggcaatacct cgaaccaccg aaggatcacg tttgggaacg gcatacaggt
     1021 cacggaggga aatactcaac aggccgaata cgataatcaa aaaccccaat cccgccccca
     1081 atacgacccg gcgtgaggac ggttgtcgct cagggttacg atccagaaag ggcaggagca
     1141 gcaatccggt aaaaaagagt gtaggaagga gccaggtggc cagaggtccc catgccccgg
     1201 gagcatattt gagaagctgg tagaaaaaca gaaaatacca ttccgggacg ggaataaatg
     1261 aatggtccga aggatccgcc cgatcagcca atgggaattc cacgctgaat gccagcagcg
     1321 atacgattcc aaacacgaca agcatcacca cggcatccat gaagacctgc cgtggataga
     1381 atgtttcact cccggctttg gcttttgctt catcatgagg accggcaggt cccaccctcc
     1441 ggagaataaa gagatggagc atgatcccga acataatcgc ggccggaaga aaagcgacgt
     1501 gaatcgcaaa aaaccgtgaa atggtcaagg cgccaagagt ttcgcctcct cgaaggattt
     1561 tcacgaggaa atcaccaata agcggaacgc tgccaaccat gttaatcccg acctgggtag
     1621 cccaataggc gttttgatcc cagggtagca aatacccggt gaatccgaac cccaacatga
     1681 tggctaacaa gatgatcccg acaatccaca tgagttcccg agggcgttta tacgcgccgt
     1741 caaaaaagac ctgcaacata tggagcccga tggccatcat gacggccgag gcgccccaat
     1801 gatgcaggcc acggacaaac catccccccc acacttcatg ttgaatatac tgaacggtat
     1861 cgtaggcatg gtcagtcgcg ggcacgtaat acaccatgag aaacatgcca gtgagtgctt
     1921 gtaatccaaa gaggaaaagg gtggcggagc cgaagatata gttccagctg gcaccgccgg
     1981 gaatgggctc attcaggagg gtttgctcca ggggctttaa tttcaggcgc tggtcgatcc
     2041 actgatacag acgtgaggcc atgcttagag ttcaactttc ttcgccagac cggatttaaa
     2101 ttcttcgtat tgaacgagca attcgccatt ttctattttg gatggcagcg tgtccagagg
     2161 gcgtggagcg ggccctgcct tcacggttcc ctcaaggtca taaatacttc catggcaggg
     2221 gcatttaaag agacggtcct gttgatccca tcgataccca cagcctaaat ggggacagat
     2281 gggtgaaaag accgtcacct ctccattggg ctgtttcacc gcccacacgc ctttcgtggt
     2341 ttgggtttcc tgccatccat ccttttcggt catggtgtaa tccatcgcct tgggttcacc
     2401 aactggtaat gcatcggctt tacccagtga catccattct ttctttcgtc gttgaaaggc
     2461 gggggtgatg atgtatccga ttaacgggac cgcaagggag attccgataa gggaggaaat
     2521 gaacacggtg gtttttacaa agaaggaccg gcgggttccc accggggtgg ataagccatc
     2581 ttccatttta gggttccttt tggataagca gacaataaga tcccggattc acttgaggag
     2641 cgtatgcttc atgctgttca cagaaaaata tcatcgttgg ggatgagaac cggaacgatg
     2701 ccgtattcag attaaagacc atttgaaaaa cgctcccgta aaaggctagc acagaagcat
     2761 ggatagtcaa aatcaaaaaa tttcatcgac agggtcgagt gtgggaagac ctgtattttt
     2821 caacgggctg actgacaccg tcgaagcatc aatgtatgga aataggtaaa gaggtggtaa
     2881 atgtgggtgc ggatcttttc gccaggggaa aatggctcct ctttccggac ttagaataca
     2941 atgtttggct tacttgcgaa gcacccggtc tgtcaggttg aacaccgttt aatatgaagg
     3001 taaaggtggc cggcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccctg
     3121 tcggccgtaa tagccgactc cacccaaccg aagcttcaca gcggcccgtc gtattttgga
     3181 aagtcgaacg ccgggaatcg agaagtccat tgcctgcccg acggtatgaa gactgtttgg
     3241 cgcggcccct cgaccaaggc gaatcagcaa tttgttgtat gagggcgacc ggtagccgga
     3301 gtagatttga atttccttcc cctgtccgac taacttttcc acctgattca catattccag
     3361 caattgaatg tccatctggc agaccttttt ggaatgatgg caccgcaaaa gataattaat
     3421 gtccttcagg gcttcctggt catagcggcc tgattgattg cgatacgtta cctggagtcg
     3481 ttcgtcagtg tgggcgttgt agaggcgaag tctgccttca gggagtgaat gggccgcggc
     3541 ggattctgga aacatcaagc ggctgccaag aatcagcatt cccgcaagag aggatttcag
     3601 aaagaagcgc cttgaccaga tttcttctgt ctcctgagac atgatgacac cctccgaaca
     3661 gtgaatgggg aaatggagcg ggagaataaa acggaatgaa acactgatca tttaacaaaa
     3721 tagaatgatg aggtcaacca atggatggaa attttttaat catcccctca cccacgttta
     3781 ttcatttccg ctgtggactg aatgaaaatg ggcaatcgcc tcctttggtt cctctgggtt
     3841 ttttacggaa actcacttgt gcggttgctg ttcattccga cgttctgcct ttaggactca
     3901 attttcagag gactcatgtc cgatatgttg aattcagcat tgatcgttga ggtgttttgg
     3961 tttgatatct ccttaccgcc atccggaacc aatgcagggt aggattttgc ttctactccg
     4021 ctcagcaaga ggggttggcg ctggaaaggc ctttggcgtt tctacatcaa acctcaactg
     4081 attgcgtgtt cctctgctta agacagagca atccctgcaa cagaaatcgc aaaatctcca
     4141 cttgatttcc gctggtcggt gggtgccggt atcgaaactc accggttcat ttcacgcctt
     4201 cccccctagc cgtaatctcc gaagtctctt tctcattcct ttgagacatg gcttttccat
     4261 tctttggtta acctcgtgcg gtaggggagg atacctgtgg atctgaaagt caatcttgtc
     4321 tcccataaga atgttcaaca aatcaggacg gaggctcagt tatctaaata gataaaaata
     4381 ttccacgatc agatacatcc ttaacgtttt aaatagaaca cgattgtcag gtgagctcag
     4441 gaggcgagtt tggaaatgct tgaaagacaa aggagataca tccgcacgac agtcgtcgtc
     4501 ccgccacatt caaatcggga ataagtcttg ctcacgtcaa taaatatatg ggcccaatta
     4561 tttaatttcc acgtatcctg taaagggtac gtcttactgt aaggagga
//
LOCUS       DAORAQ010000199         4604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2673_length_4604_cov_60.408002, whole genome shotgun sequence.
ACCESSION   DAORAQ010000199 DAORAQ010000000
VERSION     DAORAQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_4604_cov_60.408002"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..730
                     /locus_tag="PKM72_12575"
     CDS             17..730
                     /locus_tag="PKM72_12575"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNP61670.1"
                     /translation="MGVIGGKLYVIGGYKQAGLSVWQPVATVYAYDPDMDTWAERAPM
                     PTPRGALSVTAHDGKLYAIGGYDGKANSPAVEVYDPQTNTWAVRTPLPTPRDHLAAAT
                     VSGKVYAIGGRVNGDYRQNLAVMEVYDPVTDRWSRATDLPAARSGITAAEVGGRIYVF
                     GGEKEEGTFRENEAYDPARDAWQTMAPMPTGRHGLGSAVVDGRIYVISGGPTPGGSFS
                     DLNEVFTPPGSGTSKRSNP"
     gene            870..1148
                     /locus_tag="PKM72_12580"
     CDS             870..1148
                     /locus_tag="PKM72_12580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNP61671.1"
                     /translation="MEWRASHILVKEKGLADDLRKRLKTGGKFAELAKEFSTCPSGSK
                     SGDLGWFGPGKMVKAFEDAVKRLGHGSLSPVVKTQFGYHIIKKTGQKE"
     gene            1966..>4604
                     /locus_tag="PKM72_12585"
     CDS             1966..>4604
                     /locus_tag="PKM72_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61672.1"
                     /translation="MSFEKSDDSKIPVTGLLILLTVLGGFLYYQQPFRSERPVNEQQG
                     KSLIVGDKRVQSRLWQDPFASVKAHKNAEPKANQSQSEAQSSQDSPNGSGTKDHHKLG
                     IQLREITETIGTTEPNFKVLIVVTEGTPFASGTEYRLRHRYAVVSALGEAGYIPEEGE
                     FFRYFGWDRTKLKGNDTCWDESCPALDVPLEWFKPQDKQKNNVLVLWVKDQDLEKKPL
                     ASLEGLVAFVKNPEPDFRKNQQGKHSPDSTMSFTVLGPFHSGTLRAMIGEVLAKRSQE
                     PPSTSPTEGSHAIEYLSFSATASDAVLFEGFPLGREECVSEATCWEYMRSLNFDVHRV
                     IGTDEELARTLVEELTKRRVDLQNGHDHIALIGEWDTLYGRKLPLTFLEVTKTTPDLD
                     LITKIENLGDVDWPDWAHRFSYLRGIDGELPQNMNGSDGNSKGDGTDSGNFLNFRNRG
                     LERPEGPSQFDYLRRLALQIKVEDQKIRERCMDNVGEGSWPICPGFKAMGILAGDVYD
                     KLLLLQALRKEFPGVIFFTTDLDTRLLHPAQYQWTRNLLIASHFGLRLHPQIQGSIPP
                     FRDAYQTSLFLSTLLALDPVTVCRTASSFFSHGTCDDSPDTHSYKRTPRVFEVGLTGA
                     YDLSADPSTIHAARPDFEKGKPAPLISLRTFLLLGCIVLGGWVIYAAMSRRAVFLAKT
                     TCKSHMFWIFLAAIVGMYSLLLVSITDQLKDGEPFVWLEGISAWPTEVLRLAAFFLSL
                     LFLCRGYRTLQAKFRQMEEEFQLHPGQCSGWFEWKKWMEFGPDPIGSRVSPNNTVREC
                     WEVYTALMSLKCAGSRVVVQTMVYLFLFAFPIFFLIGFPTTPCRGDTCFIWDKVILLL
                     SVVTMWLLVFFVVYATRLCARMV"
BASE COUNT         1097 a         1152 c         1202 g         1153 t
ORIGIN      
        1 cctacaccat ctgggtatcg gggtgatagg cggcaagctg tatgtcattg gcggttacaa
       61 gcaggcgggg ctcagcgtct ggcagcccgt ggccacggtg tatgcctacg atccagacat
      121 ggacacttgg gcggagcgcg cccccatgcc gactcctcgc ggggccttgt cggtgacagc
      181 gcacgacgga aaactgtatg ccatcggcgg ctacgacggg aaggccaata gccctgccgt
      241 cgaggtctac gatccccaaa ccaatacctg ggctgtgcga acgccgctgc cgacccctcg
      301 cgatcatctg gcggccgcaa cggtgtccgg taaggtgtac gcaattggcg gacgagtaaa
      361 cggcgactat cggcaaaatc tcgctgtcat ggaagtgtac gatcccgtca ccgaccgctg
      421 gtcgcgggcc acagatcttc cggctgcccg cagtggaatc accgcagccg aagttggcgg
      481 tcggatttat gtcttcggcg gcgaaaagga agagggtacc tttcgtgaaa acgaggcgta
      541 tgatccggcg cgtgacgcct ggcaaaccat ggcacccatg ccgacaggac gccacggcct
      601 cgggtctgcg gtagtggatg gccgtatata cgtcatcagt ggcgggccga ctccaggagg
      661 ctcctttagt gatctcaacg aagtctttac tccacctgga tccggtacct ccaaacgctc
      721 taacccatga ggcgcaaatc agcctgacag atctggtccc tctaaacatt tgagatgaca
      781 tattttcatg gggcatcgta taatttagtc agtagatagg aagtggtgat gtttagcgga
      841 ttttaatgaa gggcgtgaaa ggagaatgta tggagtggag agcgagtcat attttagtga
      901 aagaaaaagg gttggccgac gacctgagaa aacgcctgaa aaccggtggt aaatttgccg
      961 aattggcgaa agaattctcg acctgcccca gtggttccaa aagtggggat cttgggtggt
     1021 ttggtcccgg caaaatggtc aaggcctttg aagatgcggt caaacgcctg gggcatggca
     1081 gtctgagccc ggtggttaaa actcaattcg ggtaccacat cattaaaaaa accgggcaga
     1141 aggaataatc ctcttgccag agacatcttt cgcgattcac acagatcaac tcgaacagat
     1201 gacccggagg ttttcctgcc ctacattttc gggatcatac ataaatgatg gctcgagccc
     1261 ttcaacgtga ctctcaggat aagctctaga aggctccatg cctctgctgg tcatcctcaa
     1321 acgtgtaggc cctccctagt tatcggttca ctaatgatga gcgctgtggg gctggcggtt
     1381 tgaccgcctt gcggcttcgt gattaaggcc gtcatgccga aatagccacc tgatgtgagc
     1441 ctaggccttg tcattgtcga taatggattg acgtgcttgc gaatcgttcc aacgatgaaa
     1501 ctcacgacac atgcttcttc aattaggaca caccccatag gttttcctgg cgtattgtgg
     1561 ccatatcacc aatgggctcc ctatataaat aatgaacgcg cgtttcgtca aagaatttct
     1621 ccatcacgta tctgatcgga tacaggatcg tatctgattg gatacgcaat ctcccactcc
     1681 acacatattc cattttccat tggcgttcct tttgtttctt tctttttcta taatcccgct
     1741 ggtttgtcat gtgatcagcc agtgacggag gagccttccg ttgcgctcgc atgaatcttg
     1801 agtttccagc aggtaggaac gggagaatgg ctgtcatttt ttatcgaatg gaggatgtgt
     1861 tgagttgtcc aaccacacag atgttctttc ccctcagcaa tgagacgaag ccgaaatgcc
     1921 ttatccgaca ggtatcggat tccagggaaa acttgcgagt gaaccatgtc gtttgaaaaa
     1981 agtgatgact caaaaattcc tgttacgggg ttattgatcc tgctaaccgt gcttggagga
     2041 tttctgtatt accaacaacc ctttcgaagt gaacgacccg ttaatgaaca acaaggcaag
     2101 tcgttgatcg ttggggacaa gcgggttcaa agccggctct ggcaggaccc tttcgcatcg
     2161 gtaaaggctc ataaaaatgc cgaacccaag gccaaccaat ctcagtctga ggctcagtca
     2221 tcacaagatt cccccaatgg ttccgggacc aaggatcatc ataaattagg cattcagttg
     2281 cgtgagatca cggagaccat agggacaacc gaaccaaact tcaaagtcct gatcgtcgtg
     2341 acagaaggga ctccctttgc ttccggaaca gaataccggt tacgccatag atacgcggtg
     2401 gtgtccgctc tcggggaggc cgggtatatc cccgaagaag gggagttttt ccgatatttt
     2461 gggtgggacc gcacaaaact taaaggaaat gatacctgct gggatgaaag ttgtcccgcc
     2521 cttgatgtac cgctcgaatg gtttaaacca caggacaagc aaaaaaacaa tgtcctggtc
     2581 ttatgggtca aggatcagga cctggagaaa aagcccttgg cgtccctgga aggcctcgtg
     2641 gcattcgtca aaaatccaga accggacttt cgaaagaatc aacaaggtaa acattcacct
     2701 gattccacta tgtcgttcac ggttttaggc ccctttcatt ccggaacgtt acgcgccatg
     2761 atcggcgaag tgctcgcaaa gcggagtcag gaacctccct ccacctctcc aacagagggt
     2821 tcgcacgcga ttgaatacct ttccttttct gcaacggcgt ctgatgccgt tctttttgaa
     2881 ggtttccctc ttgggagaga agaatgtgtg agtgaagcca cgtgctggga gtatatgagg
     2941 tccttgaact ttgatgtgca tcgtgtcatc ggaacagatg aagaactggc tagaacactc
     3001 gtcgaggaat taaccaaacg aagggtggac cttcaaaatg gacatgatca tattgccttg
     3061 atcggcgagt gggatacgct ctatgggcgg aaattgcctc tgacctttct tgaggtgacg
     3121 aaaacgactc cggatcttga tctgataacc aaaattgaaa atttaggaga cgtcgactgg
     3181 ccggattggg cccatcggtt tagctacctt cgtggaattg acggtgagct tcctcagaat
     3241 atgaatggct ctgacggaaa ctccaagggt gacggaaccg attccggaaa ctttttaaat
     3301 ttccgcaata gaggtcttga acgccccgaa ggcccgtcgc aattcgatta cctgaggcgt
     3361 ttggccttac aaattaaagt cgaagatcaa aagattcgtg aacgctgtat ggacaatgtg
     3421 ggggaaggat cgtggcccat ctgtccgggc tttaaggcca tgggcatttt ggcaggagat
     3481 gtctatgaca aactgctgct cctccaagcg ttacggaagg aatttcccgg ggtcattttc
     3541 tttaccaccg atcttgatac ccgtctcctc catccggccc aatatcaatg gacgcggaat
     3601 cttctgattg cttcccattt tggcctacgc cttcatcccc agattcaggg ctcgattccc
     3661 ccctttcgtg atgcctatca aacctcgtta ttcttatcca cgctgctggc tcttgacccc
     3721 gtcaccgtat gtcggacagc ttcctccttt ttttctcatg ggacatgcga cgactcacca
     3781 gacacccact cttataaaag aacgccgaga gtcttcgagg tcggactgac aggcgcgtac
     3841 gacctgagtg cggaccccag caccattcat gctgcacgac cggattttga aaagggaaag
     3901 ccggcccccc tcattagctt gcggacgttc ttactgctgg gatgtattgt attaggtggt
     3961 tgggtgattt atgcggccat gagccggaga gccgtgtttc ttgccaagac cacatgcaag
     4021 tcccacatgt tttggatttt cttggccgca atcgtcggta tgtactcctt gcttttggtg
     4081 tccatcacag accaactcaa ggatggagaa ccatttgtct ggctggaagg catcagtgct
     4141 tggcccaccg aagtactccg gttagccgcg ttctttttga gtctcttgtt tctttgccgg
     4201 ggatacagaa cccttcaggc caaatttcgt caaatggaag aggaatttca actccatcca
     4261 ggacaatgtt caggatggtt cgaatggaaa aaatggatgg aattcggccc tgatcctata
     4321 gggagtcgcg tatcccccaa caataccgtt cgggaatgct gggaagtcta tacggcatta
     4381 atgagcctca aatgcgcggg atctcgtgtg gtcgttcaaa cgatggtcta cttgtttctt
     4441 tttgcctttc cgatattttt tctcattgga tttcccacca ccccttgccg gggtgacacg
     4501 tgtttcatct gggataaggt gattctctta ttgagcgtgg tcaccatgtg gctcctggtg
     4561 ttttttgtgg tgtatgctac acggctttgc gcccggatgg tgga
//
LOCUS       DAORAQ010000200         4589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2689_length_4589_cov_64.905382, whole genome shotgun sequence.
ACCESSION   DAORAQ010000200 DAORAQ010000000
VERSION     DAORAQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2689_length_4589_cov_64.905382"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKM72_12590"
     CDS             <1..345
                     /locus_tag="PKM72_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNP61673.1"
                     /translation="LFGRAGMADPNVNRIAQYYGGGFIYKGLIPERTIDRTAIGLAAA
                     VNSHEYKQSQRRAGQPVESTEITLELTHSIFVNQNLVIQPDFQYVINPGTVPGRNNAL
                     VLGMRLEVNLNN"
     gene            complement(351..824)
                     /locus_tag="PKM72_12595"
     CDS             complement(351..824)
                     /locus_tag="PKM72_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNP61674.1"
                     /translation="MPHSRKNEIAKFLADLLDRRYSIPGTSIRIGLDPIIGLIPYVGD
                     AIVSVAGAFILVVAAQSQLPKIVLIRMSLNIAINGMIGAIPLLGDLFSIWFKSNVRNL
                     DLLERYAARDRRVSTVGDWAFVVSLLLGIAFVVVGTVVGIIWLLRYVWEILQGIQ"
     gene            complement(1377..3554)
                     /locus_tag="PKM72_12600"
     CDS             complement(1377..3554)
                     /locus_tag="PKM72_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNP61675.1"
                     /translation="MKMYNRMLLACIIILMLAFIATLSYGAETVRVSVSSGNLQANNT
                     SFLPDLNENGRLVSYYSSATNLVPGDTNGVRDIFVYDRQTQQTTRISVATGGNQANGE
                     SLDPAISGNGRYVAFRSAATNLVPGDTNGAIDVFVHDRQTGMTSRVSVATGGGQSNNN
                     SFTPSLSADGRFVAFHSFATNLVPGDTNRVLDVFVHDRQTGATTRVSVATGAIQGNNT
                     SRAGFLSANGRLVAFESDATNLVPGDTNGVRDMFVHDRQTGVTTRVSVATGGGQVNGA
                     SVDAELSADGRYVTFQSDATNLVAGDTNGVGDVFVHDRQTTTTTRVSVASGGLQGGGE
                     SSEGTISGDGRFVAFLSFATNLVPGDTNGEGDVFVHDRQTNTTTRMSVTTGGVQGNDF
                     SCNPAISQDGRIVGLFSDSTNLVPGDTNGLRDIFIHNRTTDFDLDGDGKADIVWRHTI
                     TGATAIWLMNGTTIASPRFPGGVSAVWEIVGIGDVNADGKADVIWRNSTSGTVAVWLM
                     NGHIITSVGFPGTVPTGWVLEQIGDVDGNGTADLIWQNTNSTVVVVWLMNGATIASSK
                     SLGGVPGVWKIVGLGDVNADRKTDIIWWNSNSGAVAIWLMNGLTVQSIGVPGSAILAF
                     EVAGVGDVDGNGTADLIWRNTGSGALAFWLMNGLTIGNTGSLAGKVLEWEIAQVSDRD
                     GSGKDDITWFNTNSGEVEIWLMNGVTVGSTGSPGTTSPSWEIQ"
     gene            3979..>4589
                     /locus_tag="PKM72_12605"
     CDS             3979..>4589
                     /locus_tag="PKM72_12605"
                     /inference="COORDINATES: protein motif:HMM:NF002486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HNP61676.1"
                     /translation="MGRQLTGPTHTFREKLHAEHFDFSGTPDVQDSITICMHLEKESH
                     MQCPCQSGKTFKQCCDPFITGVKQAETAEQLMRARYSAYTQVQMDFIEKTHDPKTKHD
                     IDLEANRKWAETTKWAGLEILATQQGGMNDEAGLVEFKATFETDEGPQIHHELAEFRK
                     HEGTWYFSDGKSPGVRTVVRTEAKIGRNDPCPCGSGKKYKKCCG"
BASE COUNT         1199 a         1305 c         1033 g         1052 t
ORIGIN      
        1 ctctttggtc gagccggaat ggctgacccc aatgtgaacc gcattgccca gtattatggc
       61 ggcgggttca tttataaggg attaatcccg gaacgaacca ttgatcgaac cgcgattgga
      121 ttggccgcgg cagtgaatag ccatgaatat aaacagtctc aacggcgggc aggacaaccg
      181 gttgagagta cggagatcac gttggaattg acacattcca tctttgtgaa tcaaaacctc
      241 gtcattcaac cggattttca atacgtcatt aatcccggga cggttcccgg gaggaataac
      301 gctctggtat tgggaatgcg gcttgaggtc aacctcaata attagaatgt ttattgtatt
      361 ccctgtagaa tctcccaaac atacctgaga agccagataa tccccacgac cgtacccacg
      421 accacgaaag cgattcctaa caaaaggctc accacgaaag cccaatcccc cacagttgag
      481 acacggcgat ccctagcggc atatcgctcc aacaggtcaa ggtttctgac attactttta
      541 aaccagatag aaaacagatc accgagaaga ggaatagccc cgatcatgcc gttgatcgca
      601 atattgagac tcatgcgaat caagacgatt ttgggaagct gcgattgagc agccacgaca
      661 agaatgaatg ccccggcgac actcacgatg gcatcaccca cataaggaat cagaccgatg
      721 atgggatcca agccaatccg gattgaagtc cccggaatcg aataacgccg atccagcaag
      781 tccgccagaa acttggcaat ttcatttttt cgggaatgtg gcacaataag gcttttgctc
      841 cttttattcc tgcaagatta catttgctca tcctatgagg aagattcgga ttgattttca
      901 tttcaacaga tttgacgccg aaatgtcata gacggataag ctgccaaaat gaaagaaata
      961 tctagtcaat gattcgcaaa aagggatagg tcctgtcctt tcccccatga ttgaacccga
     1021 cagattatgg aacatcattt cgcaaagctg ttcctaccgg aaacaggtga tgttgccgcc
     1081 tctcaaaaaa tgagtttaat ttttccattt ttccacaatc gtttccacca ttttcccatg
     1141 aacaacctgt ttccttggac acgatgctct tccttagcct gacaaaggaa gggggctggt
     1201 ctttggcaaa accaggcccc cgttgcactt ccacatcatg gtcgaccatt cgtatttcta
     1261 ttgttccgag tgggattcct ccagcaggcc ccagttagtt aagaaatctc ctttcaccgg
     1321 tctttagcca gtcttataac tttatttgct ccttggggct ttggttgagt ccattcctat
     1381 tgaatctccc aactcggaga ggtggtgcct ggagaacctg ttgaaccaac cgtaactcca
     1441 ttcatcagcc atatttcgac ctcacctgaa ttggtattga accaggtaat atcgtctttc
     1501 ccgctaccat cccgatcact aacctgggca atctcccatt ccaagacctt acccgccaag
     1561 gaacccgtgt tcccaatggt gagtccattc atcaaccaga aagccagcgc accgctaccg
     1621 gtattgcgcc agatcaaatc agctgtcccg ttaccatcga catcccccac cccggctacc
     1681 tcaaaggcca aaatcgcact acctgggact ccgatcgact ggaccgtaag cccgttcatt
     1741 aaccatatgg ccaccgcccc gctgttacta ttccaccaga taatatcggt tttgcgatcg
     1801 gcattgacat cacccaatcc gacaatcttc cacacacccg ggacaccgcc gagggatttg
     1861 gacgaagcaa tggttgctcc attcatcagc cacacgacga ccaccgtgct attggtgttt
     1921 tgccaaatga ggtctgccgt gccattccca tccacatctc caatttgctc aagcacccaa
     1981 ccagtgggga cggtgcccgg gaagccgacc gaggtgatga tatgcccatt cattaaccac
     2041 acggccaccg tgccactggt gctattgcgc caaatgacat cagccttgcc atcggcattt
     2101 acatcgccga taccgacaat ttcccaaacc gcagaaactc ctcctggaaa acgtggggaa
     2161 gcaatggtcg tcccattcat caaccaaatg gccgtcgccc cggtaatcgt atggcgccac
     2221 acgatgtcag ccttgccatc cccatccaaa tcaaagtccg tcgtgcggtt atgaatgaaa
     2281 atatcccgga gtccattcgt gtcaccaggg acaagattgg ttgaatcgga aaacaatccg
     2341 actatccgac catcctgact gatggcagga ttacaactaa agtcattgcc ttgcacccca
     2401 cctgtcgtca cactcatcct ggtggtggtg ttggtctgac gatcatgcac aaacacatca
     2461 ccttcgccat tggtatcgcc aggaacaaga ttcgtggcaa aagaaagaaa tgcgacaaat
     2521 cgtccatccc cgctgatggt cccctctgaa ctctctccac ccccttggag accaccactt
     2581 gccacactca cgcgcgtggt cgtggttgtt tgacggtcat ggacaaacac atcacccact
     2641 ccattggtat cgccagcgac cagattcgtc gcgtccgact gaaatgtcac atagcgacca
     2701 tccgcactca attcggcatc cacactcgca ccattcacct gccctccgcc tgtggcgaca
     2761 ctcacacgcg tcgtcacccc ggtttgtcga tcgtgaacaa acatatctcg cacaccattg
     2821 gtgtccccag ggaccagatt cgtcgcatcg gattcaaacg caaccaagcg accattcgca
     2881 ctgagaaatc ccgctcgact ggtattattc ccctggatgg cacctgtggc cacactcacg
     2941 cgggtggtcg cccctgtttg ccgatcgtga acgaacacat ccaagactct attggtgtcc
     3001 cccggcacca agttagtcgc aaaggagtgg aacgccacga atctgccgtc agcactcaag
     3061 gatggggtga aactattgtt attggactgg cccccgcccg tcgccacact aacccggctc
     3121 gtcatgcccg tctgccgatc gtggacgaac acatcaatgg ccccattggt atctcccgga
     3181 acaaggttcg tggcggccga ccgaaatgcc acataacgtc cattgccact gatggcgggg
     3241 tcaaggctct cgccattggc ctggtttccg cccgtcgcca cacttatgcg cgtggtttgt
     3301 tgggtttgcc gatcgtacac gaatatatct cgtaccccat tggtatcacc gggcaccaga
     3361 ttggttgcgg aggaatagta ggaaaccagt ctgccattct cattaaggtc tggaagaaaa
     3421 ctcgtattat tggcctgtag atttccagag ctgacactga cccggaccgt ctccgcccca
     3481 tagctcagtg tggcaatgaa tgcgagcatc aggatgatta tgcacgcaag gagcattcgg
     3541 ttgtacattt tcatgttcgt tcctccggag cccatgaatg aattttggga taaagccaat
     3601 catgaaatta ttactgctgc gaatgaccaa gtgcagcctg ctcaaattcc tatgccactt
     3661 catcgttttc actcaacgct actttccata cggagctacg tcataagatg tgcaaaaagc
     3721 ctaactcaac tgacaaataa cttccacaca tttcccattt ccctcatcgg gctttcggcc
     3781 caatcataat cctacacttt attaatagag agaaatgacc ggtctgcagg caccaagggc
     3841 tattgagccg gttgcccgcc aaaaaatcca tgctcgccat ccgggttggt acggggtatt
     3901 cttccatatc ccccgggcat ggcttacccc atatagatag accagcgatt cagagggggg
     3961 ggcgagcaga gatagggcgt gggaagacag ctcaccggac ccacccatac ttttcgagaa
     4021 aaattacatg cggagcattt tgacttttcc ggcactcccg acgtacaaga ttccatcacc
     4081 atttgcatgc acttagaaaa ggagtcgcat atgcagtgtc cgtgtcagag tggaaagacc
     4141 tttaagcaat gctgtgatcc tttcatcacc ggagtgaaac aggccgaaac ggccgagcaa
     4201 ctcatgcggg ccagatattc ggcctatacc caagtccaga tggattttat tgaaaagacc
     4261 catgacccca aaaccaaaca cgacatcgac cttgaagcca atcgcaagtg ggcggaaacc
     4321 accaagtggg cggggttaga aattctcgcc acgcaacagg gtggaatgaa cgatgaagcg
     4381 ggcctcgtcg aattcaaggc cacatttgaa acagatgaag gccctcaaat ccatcatgaa
     4441 ctcgccgagt ttcggaaaca tgagggaacc tggtatttct ccgacggcaa aagtcctgga
     4501 gttcgaacgg tcgttcgaac cgaggccaag attggacgga acgatccgtg cccctgcggc
     4561 agcgggaaaa agtataaaaa atgctgtgg
//
LOCUS       DAORAQ010000201         4543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2727_length_4543_cov_63.893717, whole genome shotgun sequence.
ACCESSION   DAORAQ010000201 DAORAQ010000000
VERSION     DAORAQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2727_length_4543_cov_63.893717"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1367)
                     /locus_tag="PKM72_12610"
     CDS             complement(267..1367)
                     /locus_tag="PKM72_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNP61677.1"
                     /translation="MAYPTEWKGSYYDGQSAVPQHVTISVTAVGLSLHFADHTSTTWN
                     YHELRQTQGRYSGEEVRFERGTGIGETLVIPGPNILLAIHEHGGSDTKHFHHPRTRGT
                     RMCWTVLAALASIPIVYGIFIWGIPLLAQPITAAIPISWEIQLGQFVQQEVTAGKPVC
                     QNPKLIHAVESILTALTDSIDHPPYRFQVTVVDSPVVNAMAAPGGYVLVFRGLLQDTA
                     SPEEFAGVLAHEIQHVLLRHTMHLIVRHVSMAFVIAALSGDASGIVAYALQAAQTLQT
                     LSYSRDAENQADEQGFQLLQQSKINPEGMITFFAKLGKEQPGNDVFRYLSTHPETQDR
                     LAHLKSLLPPGSMTYRTFPFQSEWSDITTYCK"
     gene            complement(1465..>2840)
                     /locus_tag="PKM72_12615"
     CDS             complement(1465..>2840)
                     /locus_tag="PKM72_12615"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HNP61678.1"
                     /translation="MELTPALFCTNCGRVRNGGETCSHCGVARHSPLQESVNNSNEPP
                     LATDRPLTPSESQNTLIPNRTGPLAEHGSDLAYLGSAQRKPTEPLRAIPPSNIDNESD
                     DSDLFPVHHPIFWGRGRTLFGIFIVNTFFTLLTLGLYSFWGRVRIRQFLSSQTSFAHT
                     RFSYHGTPQELLKGWSKAFLVFGLPYAFLSLVPLIWDQIPIWIPNALAATMALCFIPV
                     AVVGSHRYRLSRTSLGTIRFSFRGHVKAYMKIWILGTVLTLLTAGIYYPFFENARRKF
                     LVTHTYFGNRPFTYFGTGSGLLKIYAKALALSILIVITLIGTSAGPEGLSVFAQWPSG
                     GWRDLLFNSIFITGILTLLLPWFYLQVAKQRYQWNHSDFGDAHFQFSASTWNLVELRM
                     TNFFMLILTFGLAWPWVQVRNLQFLYYHLTLQGPLDFQRIEQEALDASPTGEELAGYF
                     DAGFDLG"
     assembly_gap    2841..2940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2941..3260
                     /locus_tag="PKM72_12620"
     CDS             <2941..3260
                     /locus_tag="PKM72_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61679.1"
                     /translation="ELAGIWAYADDTGSNTITLNEAGKGHYDWEDGWFETHELKDGVW
                     KGKWMQAGNDREGGFELKWLDDSPVARGRWWYTRIGQDHNPLEPGGTFTMRRISSFLT
                     GGE"
     gene            complement(3326..>4543)
                     /locus_tag="PKM72_12625"
     CDS             complement(3326..>4543)
                     /locus_tag="PKM72_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP61680.1"
                     /translation="AMPGRLIQALRRAGAKNPILMLDEVDKVGQDFRGDPASALLEIL
                     DPEQNHTFRDNYLDLPFDLSKVMFITTANSLDTISRPLLDRMEIIRLGGYSHQEKLEI
                     ANRYLWPRRLKEAGLESKPLTLEEQVIPHIIKRYTREAGVRQLEQMLGRLTRKVALKL
                     AEHPPDQPEESLTIIQSDLPEWLGIERFMPEEARKTLPLGVATGMAWTETGGDVLYVE
                     SALLPGGSDLTITGHIGDVMQESAQAARSYLWSRAESLGLDIAVFKQSGMHIHVPEGA
                     IPKDGPSAGVTMASALASLLLKIPVRNDTAMTGEIGLTGLVLPVGGIKEKILAAHRVG
                     LRRIVLPRANEKDLKDVPDAVRSELFVIFVESIEEALDAVLLEAPDIRERQKNPPPAQ
                     PAESEQKTVMGSR"
BASE COUNT         1107 a         1055 c         1247 g         1034 t
ORIGIN      
        1 tagcgtgtta tttcggttcc catgtgaccc accccttgtt gtgtgttgat gataataccc
       61 tccgactaga cagacggggt ataggtgtat ttggagtcgc ggtacaaaca aagacaccat
      121 acattccggt atgtctcgat tgaaatgatt atgaaggctc cagggctgga gagcaatggg
      181 ctaggtatta aaaaggtgag ttgaacctcc cgtcaagggc gtttgcgcca ggaggcaaac
      241 cgggttcgga aaatgccttt agggggtcac ttgcagtatg tggtgatgtc agaccactca
      301 gattgaaacg ggaaggttcg ataggtcatg gagcctggtg gcagcaaaga cttcaggtgg
      361 gctagccggt cttgggtctc cggatgagtc gaaagatagc gaaagacgtc gttcccaggt
      421 tgttcttttc caagtttggc gaaaaaggtg atcatcccct cgggattaat cttcgactgc
      481 tgtaataatt ggaatccctg ttcatcggcc tggttttcgg cgtcacggct ataggacagg
      541 gtttgaagcg tttgggcggc ttggagtgca taggccacta tgccgctggc atccccgctg
      601 agcgcggcga tgacgaaagc catcgataca tgcctgacga taaggtgcat ggtgtgtcga
      661 agcagaacgt gttgaatttc atgagccagg actcccgcga attcttctgg agaagcggtg
      721 tcctgcagga gaccacgaaa gaccaggaca tacccgcccg gggccgccat ggcgttgacg
      781 acggggctat ccaccacggt cacctgaaat cgataggggg ggtggtcgat ggaatcggtc
      841 agggccgtca atattgattc gaccgcgtgg atcagtttcg gattctggca aaccggtttt
      901 ccggctgtga cttcctgttg aacaaattgt cccaactgga tttcccagga aatcggaatg
      961 gcggcggtga ttggctgagc caataaggga atcccccaga taaagatgcc atacacgatg
     1021 gggatgctgg cgagtgcggc aagcactgtc cagcacatgc gtgtacctcg cgtgcgggga
     1081 tgatggaaat gcttggtgtc cgatccgcca tgctcatgaa tcgccagcag aatattgggg
     1141 ccggggatga ccagggtttc gccaattcct gtccctcgct cgaatcgtac ttcttctccg
     1201 gagtagcgcc cctgggtttg gcgaagttca tggtagttcc atgtcgtgct tgtgtggtca
     1261 gcaaagtgaa ggcttaggcc gacagctgtc acggagatgg tgacatgctg gggaactgcc
     1321 gattgtccgt cataataaga ccctttccat tctgtggggt atgccatgat cgaggtggtt
     1381 cagcctaagg gattcataat ttgtcgatgg atggtgagag gtgaatgtcg ggaaggtcca
     1441 acactgacat gcacaaaggc caaattagcc cagatcgaaa ccagcgtcaa aatatcccgc
     1501 aagctcttct ccggtgggcg aagcatccaa ggcttcctgt tcgatccgtt gaaaatctaa
     1561 agggccttga agggtgagat gataatacaa aaattgaaga ttcctgacct ggacccaggg
     1621 ccaggctaat ccaaaggtaa gaatgagcat gaagaagttc gtcatccgca actccactaa
     1681 attccaggtg gaggcagaaa attgaaaatg cgcatctcca aaatctgaat gattccattg
     1741 gtaccgttgt ttggcaacct gcagataaaa ccacggtagc agcagtgtga gaatcccggt
     1801 gatgaagatg gaattgaaca agaggtctcg ccaaccaccg gatggccatt gggcgaagac
     1861 tgacaggcct tcgggtccgg cgctggtccc gatgagtgtg atcacaatca gtatgctgag
     1921 ggcaagggct ttcgcataga ttttcaagag tccgcttccc gttccgaaat aggtgaatgg
     1981 gcgattgcca aagtaggtgt gagtgacgag aaatttccgt ctggcatttt cgaaaaacgg
     2041 ataatagatg ccggcagtca acaaggtcag taccgttccc aggatccata ttttcatata
     2101 ggctttcacg tgcccccgga aggagaaacg aattgttccc agagaagttc gactcaagcg
     2161 ataacgatgc gatccgacca cagcgacagg aatgaaacac agggccatgg ttgctgcaag
     2221 ggcattggga atccagattg gaatctggtc ccatatgagg ggaacgagac tcagaaaagc
     2281 atagggaaga ccaaaaacta aaaatgcttt tgaccacccc tttaataatt cttgcggagt
     2341 tccatgatag gaaaagcgag tgtgagcaaa actggtttgg ctgctcaaaa attggcgaat
     2401 acgcactctc ccccaaaaag aatacaggcc aagagtcagt aatgtgaaaa aggtattgac
     2461 aatgaaaatg ccaaagaggg ttcgcccacg cccccaaaaa atggggtggt ggacgggaaa
     2521 caaatcggaa tcgtccgact cgttgtctat gttcgaaggg ggaattgcgc gaaggggctc
     2581 ggtcggtttt ctctgagcag aacctaaata tgctaaatct gagccatgtt cagccaatgg
     2641 gccagtccta ttagggatca acgtgttctg agattctgat ggggtcaatg gacggtctgt
     2701 ggcgagtggt ggttcattgg agttattcac ggattcctgt agtggtgaat gacgcgcgac
     2761 cccacaatgc gaacaggtct cgccaccatt tctgaccctc ccgcaatttg tacagaacaa
     2821 agcgggcgtc agttccatcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 aagaacttgc cgggatatgg gcctatgcag acgataccgg gtccaatacc attacgttaa
     3001 atgaggcggg gaaaggacat tatgattggg aggatggctg gtttgaaacc catgaactga
     3061 aggatggggt gtggaagggc aaatggatgc aagccggtaa cgatcgggag ggagggtttg
     3121 agctgaagtg gttagatgat tcgccggtcg ctcggggacg ttggtggtac actcgcattg
     3181 gccaggatca caaccctctt gaacctggtg gtacctttac catgcgacgg atatcctctt
     3241 tcctcacagg tggggagtag tctcgtcgcg tcacgtccag aaagagagaa gaatcaaggc
     3301 tggagagaag agcatctttg aaagcttaac gactgcccat gacggttttc tgttcactct
     3361 ctgcaggctg ggcgggagga gggttctttt gtctctcacg aatgtccggc gcctccaaca
     3421 ggacagcatc taaggcctct tcaatcgatt ccacgaatat gacgaacaac tcagatcgaa
     3481 cagcgtcagg cacatctttc agatcctttt cattagctct aggaaggaca atgcggcgca
     3541 acccgaccct atgtgcggcc agaatctttt ctttaatccc gcccacaggc agaaccaatc
     3601 ccgtcaaccc aatttctccg gtcatcgccg tatcgttcct aaccgggatt ttcagcagga
     3661 gtgaagccag agcactggcc atagtcacac cggcagatgg tccatctttg ggaatggcac
     3721 cttccggcac atgtatatgc atcccgcttt gtttgaacac ggcgatgtcc aggcctagtg
     3781 attcggcgcg cgaccagaga tagcttcgag ccgcttgggc tgattcctgc atcacatcgc
     3841 caatgtggcc cgtaatggtg aggtcgctcc ctcccgggag aagggcactt tccacataga
     3901 ggacatcccc gccggtttct gtccaggcca ttcccgtggc tacccccagg ggcaatgttt
     3961 ttctggcttc ctcaggcata aatcgttcga tccctaacca ttccggaagg tcactttgga
     4021 taattgtgag agattcttcc ggctggtccg gaggatgttc tgccagcttc aaagccactt
     4081 tacgagtgag ccgtcccaac atctgttcca attggcgaac gccggcttca cgggtatacc
     4141 gtttaatgat atggggaatc acctgttcct ctaacgtcag aggtttggac tccaacccgg
     4201 cttcctttaa tcggcgaggc cacaaatagc gattggcaat ttcaagtttt tcctgatgac
     4261 tgtagccacc caagcgtatg atctccatgc gatccaagag aggtcgggag atcgtgtcca
     4321 gggaattggc cgtggtaatg aacatcacct tagagagatc aaagggcaaa tctaagtaat
     4381 tgtccctgaa ggtatgattc tgctccgggt caagaatttc taacaaggct gaggcgggat
     4441 ctcctcgaaa atcctgtccg accttatcga cctcgtccag catcaggatt ggattttttg
     4501 ctccggcacg tcggagagcc tgaatcagac ggccgggcat ggc
//
LOCUS       DAORAQ010000202         4519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2749_length_4519_cov_66.489695, whole genome shotgun sequence.
ACCESSION   DAORAQ010000202 DAORAQ010000000
VERSION     DAORAQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2749_length_4519_cov_66.489695"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..655
                     /locus_tag="PKM72_12630"
     CDS             290..655
                     /locus_tag="PKM72_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61681.1"
                     /translation="MAKLIEQPTRIKPAGNKPKLIDEYIGRVNCDTDQISIARMQSPS
                     GWEEPGQTPEFDEYTLVLSGQLQVETKEGIIEVNTGQAVIAYRNEWVRYSTPGPEGAE
                     YIAVCLQAFSPETVHRDTD"
     gene            complement(765..1304)
                     /locus_tag="PKM72_12635"
     CDS             complement(765..1304)
                     /locus_tag="PKM72_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61682.1"
                     /translation="MNTIMKQFPSKTSRLSRWIGCVLVCGVGVGLSLNDPQAFAEATT
                     IPWECSGYSGDAQDRCVITLRELQQEKIDQLAEKLKAQEGIINQLKEKLDRQEALALN
                     KAQNSKQNSRYRPPYVSPYAYSYGFSYDYAQFPPIGTYLQPPWGYPRYYGDGPGYWGY
                     GPQAFGYNFRFGGGHHHHP"
     gene            1531..2526
                     /locus_tag="PKM72_12640"
     CDS             1531..2526
                     /locus_tag="PKM72_12640"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNP61683.1"
                     /translation="MGRTTRLQTQKPIRCGWVGTKSHFIPYHDEEWGIPVHEDHRHFE
                     MLTLEGAQAGLSWSTILLRREGYRRAFAAFDPEKVARFDSRKIAVLLQDTGIIRNRLK
                     IESTVTNAKAFLTIQETFGSFDQYIWDFVGGAPIINDWKAMAQVPATTPQSDALSKDL
                     KQRGFRFVGSTVIYAHMQAAGLVNDHTTDCFRHPSNLRPQTSSQVAKVPNKSNYRAIK
                     VKRIYDTPAPDDGCRILIDRLWPRGLSKEAAKADLWQKDLAPSTALRNWFHHEPKRWE
                     EFQRRYAKELGQKPKAVEELWQRIQQGPVTLLFGAKNQECNHALALKAYLSQNYE"
     gene            3585..>4519
                     /locus_tag="PKM72_12645"
     CDS             3585..>4519
                     /locus_tag="PKM72_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP61684.1"
                     /translation="MLGNSLPMMAPTVTVEKRQPNLQKALIVALALGIFVADYLTPLG
                     LGMGSLYVLVVVATFPLNNSWTTKIATMSAVLLIFLGYWASISKPGYETAFINRALST
                     ICVLVTGYMTLKNISSRQALQTVKEDLEVKYGLQTKELKEQNHTMTVLLEDMKLIKND
                     LEDKERRLRVFVDAFPSGMLIVDHLGKVVFANKLIETLFGYSQDELMGQSIDMLVPKR
                     FQQEHARHRVAFLENPSTRAMGAGRDLFARRKNGSEFPVEIGLNPINTPDGFMVLSSV
                     VDISARKHQEAELKRLNQQLANQNQELEAYNYAVS"
BASE COUNT         1277 a         1159 c         1019 g         1064 t
ORIGIN      
        1 attcccaacc gatttctaaa gcctaaaggc aggaaccaat cgcttgaact aaacaacttt
       61 gaattcggtc tcgtccttgt tcttgctgct cgaaggtatc tgaggctatt ctaaatttga
      121 cactgatcgg acaagccgtt atgccatata aggacttcaa aagtttttcc tgagagaata
      181 caatggagat ctgtcaggcc agcaggcctg gtataaaaat ttaacgaagg caagcattca
      241 cccagtcaat gcacgcttaa attcacgaag aggaggtcaa cgagacggta tggctaaact
      301 gatcgagcaa cccacaagga ttaaacctgc agggaacaaa cccaaactca tcgacgaata
      361 catcggacgg gtgaattgcg atacagacca aatcagcatc gcgcggatgc aaagtccttc
      421 cggctgggaa gagcctgggc agacgcctga attcgatgaa tataccctgg tcctttccgg
      481 gcaactacag gtagaaacca aagaagggat aattgaggtg aataccggac aggcggtcat
      541 cgcctatcgc aatgaatggg tgcggtacag caccccgggt cctgagggag cggagtatat
      601 cgcggtatgt ctccaggcat tttccccgga gactgtacat cgcgatacgg attaaaacgg
      661 gtttccatga aagcactcag cagagacccg ggactaaacg ccgctaaaat ccttcgcgca
      721 catggcaacc gcagatgcca aactcaggct aaagatgttg atgcttacgg atgatgatgg
      781 tggcccccgc caaaccggaa attatagccg aatgcctgag gcccataacc ccaatatcca
      841 gggccatcgc cataatagcg tggataaccc cagggaggtt ggagataggt gccgataggc
      901 ggaaactggg catagtcata ggaaaaacca taggagtacg cgtagggaga aacataaggc
      961 ggccgataac gggaattctg cttagagttc tgagctttgt tcagggctaa cgcttcctga
     1021 cgatccaatt tctctttgag ctgattgatt atcccctcct gtgccttcaa tttttctgcc
     1081 aactggtcga ttttttcttg ttgaagctcc ctcaacgtga tcacgcatcg gtcttgagca
     1141 tctccagaat atccggaaca ttcccaagga atagtggtag cttctgcgaa ggcctgggga
     1201 tcattcaaag acaaccctac cccgactcca caaactagca cgcatccgat ccaacggctt
     1261 aaccgggagg tctttgacgg gaactgcttc atgattgtgt tcatgtcctc ctcctaattg
     1321 aaggcatatg agattttatc atggctttgc gtggtcctac aaacgagagg ccttcagact
     1381 aaagaactga agagtcatga agtcccgaat gatccattga cacctttttc cggaatccgt
     1441 agcatccacc ccattcccct tgcacagagg tttttattct ttaatgccag ggcacgaaga
     1501 cgtcactccc caaaaagaca ttgatttcgc atgggacgca ccacacggtt acagactcaa
     1561 aaacccatcc gttgtggatg ggtgggcacg aaatcgcact tcattcccta tcatgatgaa
     1621 gaatggggaa tccccgtcca cgaagaccac cgccactttg aaatgctgac attggaaggg
     1681 gcgcaggccg gcctatcctg gtcaaccatt ctgcttcgtc gtgaagggta tcgtcgggcg
     1741 tttgccgcat tcgaccccga aaaagtcgca cgcttcgatt ccagaaaaat cgcggtgctc
     1801 cttcaagata ccgggatcat tcggaatcgc ctcaaaattg aatcgaccgt cacaaacgcc
     1861 aaagcctttc tcaccattca ggaaacgttt gggtcgtttg atcagtacat ctgggatttt
     1921 gtgggaggtg cgccaataat caatgactgg aaagcaatgg cacaggttcc agccacgact
     1981 ccacagagtg acgccctctc aaaagatctc aagcaacgag gatttcgttt tgtgggaagt
     2041 accgtcatct acgcccatat gcaagcggcc ggattggtga atgaccacac cactgattgt
     2101 ttcagacacc cttccaacct ccgaccacaa acttcctccc aagttgctaa agtcccgaat
     2161 aaaagcaatt atcgggcaat caaagtcaaa cgaatatatg acaccccggc cccagatgat
     2221 gggtgtcgga ttctcattga ccggctatgg cctcgagggc tttcaaaaga agcggccaaa
     2281 gcggatctgt ggcaaaagga tcttgcccca tcaacagccc tgcgaaattg gtttcaccat
     2341 gaaccgaagc gatgggaaga atttcaaaga cgttacgcaa aagagcttgg gcaaaaaccc
     2401 aaagcagtcg aagagctatg gcaacgcata caacagggtc cagtcaccct tctcttcggg
     2461 gcaaaaaatc aagaatgtaa ccatgcactc gccctcaaag cctatctctc ccagaattat
     2521 gaataaaagc gccattccta taaccggcca gagcttttgg aacagagcgc atgaaaactt
     2581 gttggacaag atgatgtcac gggatgcggg ccagcttgtg tgaccacatt ggccaaggtc
     2641 tcctaccagg aaatcaaaac gatcgcactg ggaatgtgaa tcaccgtgac ggattcgcgt
     2701 ctctggtctg acatgggctc cgttcaaaca cagggccacc ataatgggat ttaagccctg
     2761 tcgagagaac aacagattgt ttcctggaag gccctgcctt cccatgtact ccttgccacg
     2821 aaatggcacc gcaaaaaaag caagcgttct gacattgggt gatctattgg aaaaattcca
     2881 accaacccgt gcttatggat catacattta ctctccgcaa acacgtccgt accgatttcg
     2941 aaaaattcga cacaaatggc ttatcgccgt ccatcattgg atttaaggaa tttctataaa
     3001 aatattaatc aaatttttca cttttgattg aacgcatttt ccttgcgtac attaaaaaaa
     3061 accgttacta tcccgcatta tttcataatt agcctcattt cacattcatc ctgccttatt
     3121 tttatcatgt cgagagctgt cacaatcccg atccaatcac tcaggggatg atgcagtatg
     3181 atcgaggatt cgccaatgta ccggcagatt caacattctc ctcaaaaatc ggcgtgatac
     3241 aacaaccatt tctctcaggt gcgaatttat gatttccgtg ggtcaacatt aactaaaggg
     3301 ctaaggatat ttgggtccac cttttgaccc aaacaagtcc tccgtcgggg ctccacatta
     3361 tcaccatgcg gcacgctgga gcatgaaccg tttcgtccaa tttctgagcc atagacgacc
     3421 ttcagtcggt atggttaggg gattcagtcc gccatctcac ccagctccag cagtcctaga
     3481 aacacgacac accgaaatat cttttcccca tgtaccgaat cctccggtac tccctgtcat
     3541 ggctcattca ttgaatgaac caatggaaaa agcaagtaga taatatgtta ggcaattctc
     3601 ttcccatgat ggctcccacc gtaacagtcg aaaagcgaca gccaaatctt cagaaggctc
     3661 tcattgtagc gctggcattg ggaattttcg tagcggacta cctgactccg cttggacttg
     3721 gtatgggcag tctgtacgtt ctggtcgtcg tggccacttt ccccctcaat aattcatgga
     3781 ccacaaaaat cgcgaccatg agcgccgtcc tcttaatttt tctgggctat tgggcctcta
     3841 tttcaaagcc aggatatgaa acagccttta ttaatcgcgc cctatcaaca atctgcgtct
     3901 tggtaacagg gtatatgact ttaaaaaata tctcttcccg ccaggcactc caaacggtca
     3961 aggaagatct cgaagtcaaa tatggcttac aaaccaagga actgaaagag caaaatcaca
     4021 caatgacggt tctgctagaa gatatgaaat tgatcaaaaa tgatcttgag gacaaggagc
     4081 gtcggctacg cgtgtttgtt gatgccttcc caagtggcat gttgattgtc gaccatctcg
     4141 gcaaggtggt ctttgccaac aaattaattg aaactctctt tgggtattca caggatgaat
     4201 taatggggca atccatcgac atgctcgttc ccaaaagatt tcagcaggaa catgcccgac
     4261 atcgagttgc atttctcgaa aacccaagta cccgagccat gggggccgga cgggacctgt
     4321 ttgcccgacg gaaaaatgga agcgagtttc ccgtagaaat cggcctgaat cccatcaaca
     4381 cgccggacgg cttcatggtg ctcagttcag tcgtagacat ttcagccaga aaacaccagg
     4441 aagctgagct caaacgctta aaccaacaac tggccaacca gaatcaggaa ctggaagcct
     4501 ataactatgc cgtttcaca
//
LOCUS       DAORAQ010000203         4510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2753_length_4510_cov_64.418855, whole genome shotgun sequence.
ACCESSION   DAORAQ010000203 DAORAQ010000000
VERSION     DAORAQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_4510_cov_64.418855"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PKM72_12650"
     CDS             complement(<1..1306)
                     /locus_tag="PKM72_12650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNP61685.1"
                     /translation="MSKKAFLIIIVLALIAIYLGLIGNARWGDSTAPEISLEQPFEQV
                     GPTTPLVLRIHDPETGLRDISVRIIHNLETFVLADQSFPSAGWLSATGGHDHEFTLDV
                     IPYADGDLPRRQGQAQLIVTARDYSWRNWFEGNGQRLEKEFTPQFTPPRLELLSAPTT
                     IVQGGAGIVRYRVIPEVSIHGVKIGKAFFPGYPAPDKAGMFAFIAFPYNLPSSTPIQV
                     IADDGFGNSAIMDVNFKVKPQAWRTRSINISESFIRKTVPSILAQTPELQSLGDSLKD
                     FLLVNDKLRKMNNDTITNLAKVSRKEFLWKGAFRQLSGSKVEASFADHRKYMYNGQVV
                     DTQDHLGFDLAVTQHYPVEAANHGIVVFAGYLGIYGNTIILDHGYGLLSLYAHLSSIE
                     VKTGEAVTLGQPIGKSGTTGLAAGDHLHFSLILHGEQVNPTEW"
     gene            1778..2806
                     /locus_tag="PKM72_12655"
     CDS             1778..2806
                     /locus_tag="PKM72_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNP61686.1"
                     /translation="MPGLSDILGWFSSDLAIDLGTATTLVYVRGKGIVLSEPSVVAIE
                     KQSNTVLAVGVEAKRMVGRTPGNIVAIRPIKEGVIADFAMTERMLHYFITKAHNRRAF
                     VRPRCIIGVPSRITEVEQRAVRESATQAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     IDIGGGTTDIAVISLGGIVCSESVKVAGDSMDDAIMSYIKRRYNLLIGDHMAEKVKIE
                     VGSAYPLEQAKTMMVKGRDMISGIPRTVVVNDSEIREALEDPIHAIVNALRNALENTP
                     PELAGDIIDKGVVITGGGSLLPGIATRFQEETNLPIITVEDPLTSVVYGVGKALDEVD
                     LFQKIQKF"
     gene            complement(2814..3950)
                     /locus_tag="PKM72_12660"
     CDS             complement(2814..3950)
                     /locus_tag="PKM72_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNP61687.1"
                     /translation="MGIIIGIAVGLWSTLSWSLSGKPAPANGPIGLQPVVEHEFSKPV
                     FLTGSPDQTKRLFVVEQHGRIVILTPGQRDPSLFLDITEKLSTGGERGLLGLAFHPQY
                     STNGRFFVNYTRARDGATVIAEHQVSPAPNQANPQETIRLVIPQPYSNHNGGMIAFGP
                     DALLYIGMGDGGAGGDPENRAQDRQSLLGKILRIDVNGPPPYRIPADNPFIGQQGQPE
                     IFALGLRNPWRFSFDRQTGELWAGDVGQNLWEEIDVIAKGKNYGWRLFEGSHCFKPSK
                     NCELAEGVVRPVTEYRHEQGRCSVTGGYVYRGKHVPWLEGVYVFGDYCTGEIWGYRDG
                     QTTQLLDTDLQIASFGEDREGEIYVVGHQGKIFQIVPNATAGLP"
     gene            4097..4291
                     /locus_tag="PKM72_12665"
     CDS             4097..4291
                     /locus_tag="PKM72_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61688.1"
                     /translation="MILKYESDILDDENARGNFYHVCQGKVQDTPVGIPPDDQPYECP
                     ACGIDLEQEDFMLAQQRGWS"
BASE COUNT         1089 a         1059 c         1241 g         1121 t
ORIGIN      
        1 accattccgt gggattcacc tgttcgccat gcaaaatcaa actaaaatgc aggtggtctc
       61 cggcggcgag gccggtggtg cctgatttgc caatgggttg gcccagagtg acagcctcac
      121 cggttttgac ttcgattgag gacagatgtg catacaaaga aagcaacccg tatccatgat
      181 ctaaaataat tgtgttccca taaattccca ggtatccggc aaacaccaca attccgtggt
      241 tagcagcttc gacgggataa tgctgggtaa cggcaaggtc aaaccctaaa tgatcctggg
      301 tatcgaccac ctgcccgtta tacatgtatt tgcgatgatc ggcgaaggag gcttcaacct
      361 tggaccccga cagttggcga aacgccccct tccagaggaa ttccttgcgg ctaaccttgg
      421 ccaaattggt aatggtatcg ttattcatct tgcgtaactt gtcgttgaca agaaggaaat
      481 ccttcagcga atcacccagg ctttgtaact cgggggtttg agccaggatg gaggggacgg
      541 tttttcgaat gaaggattcg gaaatattta tggagcgggt acgccaggct tgaggtttga
      601 ccttgaaatt cacatccatg atggcggagt tgccaaatcc gtcgtctgca atcacttgaa
      661 tcggtgtcga ggacggcaaa ttatagggaa aggcaatgaa ggcgaacata ccggccttgt
      721 caggtgcagg atatcctggg aagaaggcct ttccaatttt taccccgtga atagacactt
      781 ctgggattac tcgatacctc acaattcctg ctccaccttg cacgatggtc gtgggggccg
      841 ataggagttc caatcggggc ggcgtgaatt gtggggtaaa ttccttttcc agccgttgtc
      901 cgttaccttc aaaccagttt cgccaggaat aatcccgtgc ggtcacaatg agttgagcct
      961 gcccttgtcg tcgcggcaga tccccgtcgg catagggaat gacatccagc gtgaattcat
     1021 ggtcatgtcc gcccgtcgcc gaaagccatc cggcagaagg aaaggactgg tcggcgagaa
     1081 cgaaggtttc caaattgtga atgatgcgaa cggagatgtc gcgaaggccg gtttcaggat
     1141 catgaatacg gagaaccagg ggtgtggttg gaccgacctg ctcgaatggt tgctcgaggg
     1201 agatttcagg agcagtggaa tctccccatc gtgcgttccc aattaatccc aggtaaatgg
     1261 cgataagagc caggacgata ataatcagaa aggctttttt ggacacgatt ggggtaactc
     1321 tttttccaga ggattatgcg agcaggacaa aaaaatgttc ctcttcttat accataccca
     1381 gccttcagag caagtgtgtg gcgcgtttga aatgacggtg aagcgggcga ctgtgaatta
     1441 cgaccttaga aatggctgct ctgtactctt gacatggaca ggatgatcgc ctagcctgtt
     1501 ccgggcttca gtcaggccct aagtctcata tccctcctct cgggcaatgt attccgtcaa
     1561 gatggagatg tgggaattcc cgctccttga ataattgatc aggagtttgg tttcgaggaa
     1621 gaagccggtg ggttcttggt tccaaacaca ttcccgaaca ttttctcgat atttctttat
     1681 tttgacaaat ggtgttccga tatatgccgg tagtgccgaa ccggcttcag cctgatcggc
     1741 attatttgaa tcaaccgtct ttccacatgg ggggctcatg ccagggttga gtgacattct
     1801 agggtggttt tcgagcgatt tagccatcga tttaggaacg gccacgacac tggtctatgt
     1861 tcgtggaaaa gggatcgtcc tgagtgaacc ctcggtggtg gctatcgaaa aacaatcgaa
     1921 tacggtgttg gccgtgggcg tcgaagccaa gcgcatggtg ggaaggacgc cgggaaatat
     1981 tgtggcgatc cggccgatca aagaaggcgt tatcgccgat ttcgccatga ccgagcgcat
     2041 gttgcattat tttattacca aagcgcataa ccggcgggcg ttcgtccggc ctcgctgtat
     2101 tattggtgtc ccatcccgta ttaccgaagt tgagcaacgt gctgttcgtg aatcggccac
     2161 tcaagcgggc gctcgggaag tctatctcat tgaagaaccg gttgcggcag ccatcggtgc
     2221 cgggttaccg atcacggagc cctcagggaa catggtgatc gacattggag gaggtacgac
     2281 cgatattgcc gtcatttccc ttgggggcat tgtctgcagc gagtcggtaa aggtggcggg
     2341 tgattccatg gatgacgcca tcatgagcta tatcaagcgt cggtataatt tgctgatcgg
     2401 tgatcacatg gcggagaaag tgaaaatcga ggtcggatcg gcctatccgt tagaacaagc
     2461 caaaacgatg atggtcaaag ggagggatat gatttcaggt attcccagga cggtggtggt
     2521 gaatgactct gaaatccggg aagcgttgga agatcccata catgccatcg tgaatgcctt
     2581 acgaaacgcg ttggagaata cgccgccgga gttagccgga gatattatcg ataagggtgt
     2641 ggtgattaca ggcgggggtt cgttattgcc gggcatcgcc acgcggtttc aggaggaaac
     2701 caatctgccc atcattacgg tggaggatcc tttgacctcc gtggtgtatg gcgtgggaaa
     2761 agcccttgat gaagtcgatt tatttcaaaa gattcaaaag ttttaatggt ggtctagggc
     2821 aatccggcag tggcgttggg tacaatctga aaaatcttac cttgatgacc gaccacatag
     2881 atttctccct cgcgatcttc tccaaaggac gcaatttgta aatccgtatc cagcagttgt
     2941 gtggtctgcc cgtcgcggta gccccagatt tcaccggtac aataatcccc gaacacatag
     3001 actccttcta gccatggcac gtgtttccca cgatagacat agcccccggt caccgaacat
     3061 cgtccctgtt cgtgccgata ttctgtaacc ggacggacca caccctcggc caattcgcag
     3121 tttttgctgg gcttgaagca gtgactccct tcaaagagtc gccatccata gtttttccct
     3181 tttgcaatta catctatttc ttcccaaaga ttctgtccca catccccggc ccataattcc
     3241 ccggtctggc ggtcaaagga aaaacgccag gggtttcgaa ggccaagggc aaaaatttca
     3301 ggttggcctt gttgcccgat aaatggattg tctgcaggga tgcggtaggg agggggtcca
     3361 ttcacatcaa tccgaaggat ttttcctagc aatgactgtc ggtcttgcgc tcgattctcc
     3421 ggatccccac ctgctccgcc gtctcccatc ccaatgtaca acaatgcgtc cgggccaaag
     3481 gcgatcatgc caccgttatg gttgctgtag ggttgtggaa tgacgagtct gatggtttcc
     3541 tgagggtttg cttgattggg ggctggtgaa acttgatgct cagcaatcac cgtagcgccg
     3601 tcccgtgccc ttgtgtagtt gacaaaaaac cggccattcg ttgagtactg aggatggaat
     3661 gccagtccca ggagccctcg ttcccctcct gtggacagtt tttctgttat atccaagaaa
     3721 agagacggat cccgttggcc tggagtcagg ataacaatgc ggccatgttg ttctaccacg
     3781 aacaagcgtt tggtctgatc cggactgcct gtaagaaaaa caggcttgga aaattcgtgc
     3841 tccaccacgg gttgcaatcc aatgggtcca ttggcaggag caggttttcc agaaaggctc
     3901 caactcaacg tggaccaaag gcctaccgct atccctatga tgattcccat cgtcaggggg
     3961 cctcgtcgcc tttgaggcgg tcgatggaga cgaaatgggt gttttctgga aatgatggag
     4021 gggaatgggg attgcccggg aaatcgacgc cttgttataa cttgagaaac cataagttaa
     4081 tgtaaaggag agtgagatga tcctaaagta tgagtccgat attttagatg acgaaaatgc
     4141 cagagggaat ttctatcatg tctgtcaggg aaaggtccag gacacgccgg taggcattcc
     4201 tcctgacgat cagccctatg aatgccccgc gtgtggcatt gatctggaac aggaagattt
     4261 tatgttggcg caacaacggg gctggtccta attgttagga gaggccaccc ccatcttatt
     4321 gtagggagga ccagatggag ttaccacgta gccacgagag aaaaacggta tcggtcatgc
     4381 gcggccgcat gatgctgtgt tcggcttgtt tcgacagcat tcatgaagaa aacttagtgg
     4441 atgaaaaaaa ctatgtggca gaccatgagg ctttttttga tcttatttgt ccaggctgcg
     4501 aagatttgaa
//
LOCUS       DAORAQ010000204         4492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2771_length_4492_cov_71.098715, whole genome shotgun sequence.
ACCESSION   DAORAQ010000204 DAORAQ010000000
VERSION     DAORAQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_4492_cov_71.098715"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..802
                     /gene="ubiE"
                     /locus_tag="PKM72_12670"
     CDS             53..802
                     /gene="ubiE"
                     /locus_tag="PKM72_12670"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNP61689.1"
                     /translation="MNKIEIPGKAASTWTGPAREQAVQRMFTAIAKFYDLNNSLLSFG
                     LHHSWKRKAISYIPDTSGTRALDLGAGTCDLAILVDQHLRYTSQIIAADLNLAMLRVG
                     QEKVRSQKLANRITCLQMNAEHLTFPSATFDTVTAGFCIRNVGNLPQALSEICRVLQP
                     GGRFVCLEFSRPVSAGLRKLYDWYSFHLLPWIGTKVARDGTGVYEYLPASIRNFPDQN
                     RLAQLLKEAGFQTVEYHNLTGGIVAIHVAVK"
     gene            807..2438
                     /locus_tag="PKM72_12675"
     CDS             807..2438
                     /locus_tag="PKM72_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP61690.1"
                     /translation="MNSVLYVFLPCKKVYPTGITYLADFIHRRRPDVRQQILDLSLFP
                     PNQRQAQLREATKAFQPELVCFSWRDIQIFSPHEGDASLEHAFNFYYASNPLKRVVAS
                     FQGLHNLYRYYTDIRHNLSYPWLIQKEFPSTGMMIGGGAFTAFADQLIEKLPEGIIGI
                     LGEGEDAILKVLNGEPLGEERFIIKEQGKVTKGTKNTPALLDALSVDIPYLTSIFPQY
                     REYIGECIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPAENVVKDIAQYYHQWGARRFW
                     FTDAQFITGKDAYPQCTEILERIISQELHIEWSGYVRTSLINPTLAKLMVQSGVGDLE
                     VAITAGSQKVLNSMHMGFKLEHLYDGCRYLKEAGFNGRVILNYSLNSPDETEETLLQS
                     IESYKMVASILGEDRVFPLMFFLGIQPNTDLEQRLLSEGYLSKGYNPLSLTPWNIKKM
                     LYNPAPLNKIIAKACLAAWGRKHGSQDPRPWSGSLSQSATQQQGHAYADSSLSKGFET
                     NSGRDALLTLEEILRSRRPAASPPLSPASQPNPSR"
     gene            complement(2811..3446)
                     /locus_tag="PKM72_12680"
     CDS             complement(2811..3446)
                     /locus_tag="PKM72_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61691.1"
                     /translation="MYTAQKFFTTLIRKNVNGFHFALPAAAFTLLFLVSNVSGADDPF
                     GSSTLSPNKNDMLKDMAYDQVNWKRPQGVFTWITMATGYDTPWDSLGRPGWMTTGDAY
                     VEPVNPGISEFPLDTQMFYIVFEAQPLDAPGQFAIAWFHMKDGQVASNTPVGQDVIEL
                     EMNQRSGYFQVSKPADGWIPGKYQVKIFYGSPGQALHAVNVIGTMEFTIKG"
     gene            complement(3584..>4492)
                     /locus_tag="PKM72_12685"
     CDS             complement(3584..>4492)
                     /locus_tag="PKM72_12685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNP61692.1"
                     /translation="GPNKAECSSMLTQPHNIIAIIYDFDHTLSPHYMQDHTILRHAEL
                     DPATFWPSCTDLIKEKGYDQELAYMKRMLEYDQIRSLSNQDLKSMGNLLEFFKGVPEC
                     FGELNTILKKPKYEEFGIRLEHYVVSSGLKAILDGSVVATHVKAIFGCEFDEENGHIS
                     FPKRTISHTQKTQFLFRVNKGLLNLDQDVNDHMPEESRRVPFPQMVYVGDGPTDVPCF
                     TLMKKNDGFALAVYNPEDQTRRSFEKCYQLTRHANRVHFMAPADYRPGSHLRLILEKH
                     ITEVADRMVDSRRQSVEGSRIPAPLP"
BASE COUNT         1073 a         1090 c         1172 g         1157 t
ORIGIN      
        1 attcttgaca aaacttgaga tcgggtgcta ggtccttgag gtggttcctg tcatgaataa
       61 aattgaaatt ccaggcaagg cggcctccac atggacaggt ccggctcggg agcaggccgt
      121 ccagcgaatg ttcacggcca ttgccaagtt ttatgacctg aataattccc ttctcagttt
      181 tggtctgcac cattcctgga aacgcaaggc gatttcctat atccctgaca cctctgggac
      241 acgagcctta gatttaggag cggggacctg cgaccttgcc attttggtgg atcaacatct
      301 ccgctatacg agccaaatta ttgcggcgga tttgaattta gccatgttgc gtgtggggca
      361 ggagaaagtc cggtcccaga aattggccaa ccgtattacc tgtttgcaaa tgaatgccga
      421 gcatctcacc tttccgtctg ccacatttga tacggtgact gccggatttt gtattcggaa
      481 cgtcgggaat cttccccaag cgctctccga aatctgccgg gtcttgcaac ccggaggtcg
      541 cttcgtgtgc ttggaatttt cccgtcctgt ttcagccggc ctgcgcaagc tgtacgactg
      601 gtattccttt catttgctcc catggattgg caccaaggtg gcccgtgatg gcacgggggt
      661 gtatgaatat ctcccggcgt cgatacggaa ttttccagat caaaaccggc tagctcaatt
      721 actcaaggaa gccgggtttc agactgtgga gtatcacaat ctgaccggtg gaattgttgc
      781 cattcatgtg gcggtgaaat agcacgatga actccgtatt gtacgtcttt ctcccctgta
      841 aaaaggttta tcccaccggg attacctacc tggccgactt catccaccgg cgccggccgg
      901 atgtgcgtca gcaaattctg gatctctcct tatttccgcc gaatcaacgt caagcccaat
      961 taagagaagc gaccaaggcc tttcagccgg aattggtgtg tttttcatgg cgggatatcc
     1021 aaattttttc cccgcatgaa ggtgacgcct cgctcgagca tgcgtttaat ttttattacg
     1081 ccagcaatcc cctgaaacgg gtggtagcct ctttccaagg tttacacaat ttataccgtt
     1141 attatacgga tatccggcat aatctttcct atccctggtt aattcagaag gaattcccct
     1201 caaccggaat gatgattggc ggcggagcgt tcaccgcctt tgctgaccag cttattgaaa
     1261 aactgccgga aggtatcatt ggtattctgg gagaaggaga ggacgccatc ctcaaagtcc
     1321 tgaacggcga gcctcttggt gaagaacggt tcattattaa ggagcaggga aaggtcacca
     1381 agggcaccaa aaatactcca gctttgttag atgcgctctc cgtggatatt ccttatctca
     1441 cgtcaatctt tccccaatac cgtgaatata tcggcgaatg tatcggtgtg cagagcaaac
     1501 gtggctgccc gtatgattgc gccttttgtc tctatccgta tattgaaggg aagcgggtac
     1561 gctaccgtcc ggcggaaaat gtggtcaagg acattgccca gtattatcac caatggggag
     1621 ctagacggtt ttggtttacc gacgcgcagt tcatcaccgg caaagatgcg taccctcaat
     1681 gtacggaaat tttggaacga atcatctctc aggaattaca cattgaatgg tcgggatacg
     1741 tccggacctc gctgattaat ccaacgttgg ccaagctgat ggtgcagtca ggagtgggag
     1801 atttggaagt ggcgatcacg gcgggctcgc aaaaggtcct gaatagcatg catatgggct
     1861 tcaagcttga acatttgtat gacggatgcc ggtatttgaa agaggcggga ttcaatgggc
     1921 gggtcattct gaattattcg ctgaactcac cggatgaaac agaagaaact ctccttcaaa
     1981 gtattgagtc ctataaaatg gtggcctcga tattgggaga agaccgggtg tttcccttga
     2041 tgtttttctt aggaatccag cccaatacgg atttggagca acgattattg tcggaagggt
     2101 atttatcgaa gggctacaac ccgctcagtt tgacgccatg gaacatcaaa aagatgctgt
     2161 acaatccggc cccgcttaac aagattatcg ctaaagcttg tctggcggcc tggggtcgga
     2221 agcatgggtc gcaggatccc cgcccctggt caggcagctt gagccagagc gcgacgcaac
     2281 agcaggggca tgcttatgcc gattccagtt tgtctaaagg ttttgaaacc aactccggcc
     2341 gtgatgccct tttgactttg gaagaaatcc tccgttcccg tcgccctgcg gcctccccac
     2401 ccctctcccc tgcttcccaa ccgaatcctt cgcggtaatt attacgatat tggaattgat
     2461 gataaatggc gggtttcggc ctagccgtcg aagtccttag ggtttcgaaa aaggatccca
     2521 aacccttgac cgtcatccgg cctcatctga ttgggaggac gcaacctata agtgagggcg
     2581 gagcgattcg cttttcccag actcggtcga ctggttcttc cgagcgtcca cgcagaggtt
     2641 cgggcggcac atgggtcgag gaggggaaac aattgggatt gagaggccta gagaactcca
     2701 ggtcgttatt cagcaggtgc aagggtgttg cggacaatta cccgttggtc ggtaactaag
     2761 caaaaagatt tcggcaattc tcctggaagg actgtaattc ctacgagtat ttaccctttg
     2821 atcgtgaact ccatggtgcc gatgacgttg acggcatgca gcgcctggcc tggcgagcca
     2881 taaaaaatct tgacttgata ctttcccggt atccatccat ccgccggttt tgaaacttga
     2941 aagtatccgg atcgttgatt catttccagt tcgatcacgt cttgtccgac gggcgtattc
     3001 gacgccactt gaccatcctt catgtgaaac cacgcaatcg cgaattggcc aggggcgtct
     3061 agtggttgtg cctcgaagac gatgtaaaac atctgcgtgt ctaaggggaa ttccgaaatg
     3121 ccagggttaa cgggttcaac atacgcatcc cccgtggtca tccatcctgg ccgtccaagg
     3181 gaatcccagg gtgtgtcgta tcccgttgcc atggtaatcc aggtaaagac cccttgtggg
     3241 cgcttccagt tgacctgatc atacgccatg tctttgagca tatcgttttt gttgggagac
     3301 agggtcgagc tgccaaatgg atcatcggcg ccactcacgt tactgaccag gaacaggaga
     3361 gtgaaggctg ccgcaggcag agcgaagtga aaaccattga cattcttgcg aattaaagtg
     3421 gtgaaaaatt tctgtgctgt gtacatgttc cacctcctgg tgggaaggtc gcttttgtta
     3481 taagacattt aactcctttt accttactcg accgaaaata ccttatgcaa tgaggtaccc
     3541 gagaccaagg aatgagggag gccggggata ggataaagaa taattatgga agaggggcgg
     3601 gaatgcggga gccttcaacg ctttggcgtc gactatctac catgcgatcg gcaacctcgg
     3661 taatgtgctt ttcaagaatc aaacgcaaat ggctaccggg tcgatagtcc gccggcgcca
     3721 tgaaatgaac acggttggca tgccgggtaa gctgatagca tttttcaaag gatcttcggg
     3781 tttggtcctc aggattgtaa accgccaagg caaagccgtc gttcttcttc atgagagtga
     3841 aacagggaac gtccgtgggt ccgtccccca catacaccat ttggggaaat ggcactcggc
     3901 ggctctcttc cggcatgtga tcattcacgt cctggtcgag attgagtagg cccttattca
     3961 ctcgaaagag aaactgggtt ttctgggtat ggctgatggt gcgtttggga aaactgatat
     4021 ggccgttttc ttcatcaaac tcgcatccga aaatggcttt gacatgggta gccacgacgc
     4081 ttccatccaa aatggccttt agacctgaac tgaccacgta gtgttcaagc cggatgccga
     4141 actcctcata tttcggtttt ttgagaatgg tattgagttc accaaaacac tcaggaactc
     4201 ccttgaaaaa ctctaaaagg ttgcccatgg acttgagatc ctgattcgac aagcttcgaa
     4261 tctggtcata ttccaacatg cgtttcatgt aggccagttc ctggtcgtag cctttctctt
     4321 tgattaaatc ggtacagctg ggccaaaagg tggcaggatc aagttcggca tgtcgcagga
     4381 tggtatggtc ctgcatgtaa tggggactca acgtatgatc gaaatcatag atgatggcaa
     4441 tgatgttatg cggttgggtc aacatagagg aacactcggc cttgtttggt cc
//
LOCUS       DAORAQ010000205         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2774_length_4483_cov_63.509033, whole genome shotgun sequence.
ACCESSION   DAORAQ010000205 DAORAQ010000000
VERSION     DAORAQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2774_length_4483_cov_63.509033"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1058
                     /locus_tag="PKM72_12690"
     CDS             303..1058
                     /locus_tag="PKM72_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61693.1"
                     /translation="MRKVGYFGLAMIFAGSVSVAVAQERQPQHVGYGTGVGESQGVGD
                     RSRVMDQDDMLRYLSANETIQGEVMAIDLSSHKLYLIKGGSSQDERQTLRGSLNIMTL
                     YLDDKTNYENLKAIGKGDYVTVQAENETTETQKYGTGRKLVRDVYVLDGSQLLGGIDN
                     PNFKGGFGQGPDPNMAQGQNIMNDSMTGVPAGAVQPGSVASGIVSELGVMTGAAPCWQ
                     CAPQPTTVNEKTDKTVATDYGDDRKNYNKGTVQ"
     gene            1198..1422
                     /locus_tag="PKM72_12695"
     CDS             1198..1422
                     /locus_tag="PKM72_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNP61694.1"
                     /translation="MEELDLRGVICPYNFVKTKLKLESLEVGSQLRVLLDEGEPIRNV
                     PQSITNEGHQILKQEKVEHYYLVVIQKVGV"
     gene            complement(1404..2150)
                     /gene="rsmI"
                     /locus_tag="PKM72_12700"
     CDS             complement(1404..2150)
                     /gene="rsmI"
                     /locus_tag="PKM72_12700"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNP61695.1"
                     /translation="MTGTLYIVSTPIGNLEDMTFRAIRILKEVAIIAAEDTRRTQKLC
                     TYYHIGTPLTSYHDFNKEEKTEILLHRLQEGSSVALVCDAGTPLISDPGFYLVRQAIK
                     ANIPLIPIPGPSSVLTALCVSGLPTDRFIFEGFVPRKPGAREKFFQNLKEEKGTIIVF
                     ETQHRILKTLQVITTVMGKRNIVLTRELTKINEEILRGEIEEVTQQLKAKSIKGEITL
                     LIQGVPQRSKRQFEPEPESTSSSAHHTPTF"
     assembly_gap    2405..2504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2777..3586
                     /gene="ccsA"
                     /locus_tag="PKM72_12705"
     CDS             2777..3586
                     /gene="ccsA"
                     /locus_tag="PKM72_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNP61696.1"
                     /translation="MPELLPFLTMGFYFGGTLLFLLYLWHRSDVIAKISLVATGLGFF
                     SHTSALIMALVSTGHIPIMTFKDAMSFFAWGLVLVFLVVGLKRGLAVLGAFILPLAFL
                     SLVSATLAPQSSGTLAPVFQTVWVHVTLSILGTIGFAVAFVAGLMYVMQERLLKSKQF
                     NVLSFKLPPLDFLDSLNQRSILFGFPLLTLGILTGAVSAQLTIGSYLSWNSEQIWALI
                     TWFFYFAVLMGRVTAGWRAKKAAYLTIVGFAGVILTFVGILIKSPQPMSPL"
     gene            3583..>4483
                     /gene="hemA"
                     /locus_tag="PKM72_12710"
     CDS             3583..>4483
                     /gene="hemA"
                     /locus_tag="PKM72_12710"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNP61697.1"
                     /translation="MNIIVLGLNHRTASVELRELLAIQDSRMGEALGRLMAYSGIKEA
                     MYLGTCNRVEVYAVVENGEWGFRQLEDFLVSTHFSLSSEDLLPHLYRYSEDEAIAHLF
                     RVSASLDSMVVGEPQVLGQVKEAYELALTHRSSGVILNKVVKKAISVGKSVRTNTRIG
                     EYAVSVSYAAVELAKKVFSNLKQRVVLLVGAGEMGKLAAQHLVNQGVKEVLITNRNHH
                     RAFALAERFHGQAVPYEQLRSTLPKVDIVICATGAPHYVITKDDIEMAVYHRMNRPMF
                     LIDITVPRNIDPAVKDVDNAYVFD"
BASE COUNT         1078 a          847 c         1134 g         1324 t
ORIGIN      
        1 ctcaaaatcc ggttctttca cgagagtggg agttcgaccc tccctccggg caccagcata
       61 acttctgtaa gatatttttg tgacatggat aaagtaaaat tcatcatagg ttacatatcg
      121 gattcgggtg agattcatca ggggtaattc gagtatggag aatggagaat tggtgtcaaa
      181 tattgacttg acaggggcta tcgccttgac tatagttggt gcccttgatc ctgaaataaa
      241 atcgtatatt tgtattagtt cgtttttttg attattaact cacacttcca aggaggagcc
      301 acatgcggaa ggtagggtat tttggattag caatgatttt tgcaggctcg gtcagcgtgg
      361 cggtggcgca agagcgtcaa ccacagcatg tagggtatgg tacaggggtc ggggaatccc
      421 agggtgttgg tgacagatca cgggtgatgg accaagacga tatgttacgg tatctctcgg
      481 caaatgagac cattcaggga gaagttatgg ctattgatct gtcaagccac aagttgtacc
      541 tcattaaagg ggggagttcg caggatgaac ggcagaccct cagagggtct ttgaacatca
      601 tgaccttgta tttagacgac aaaacgaatt atgagaattt gaaggcaatt gggaagggcg
      661 actatgtaac cgttcaagcg gagaacgaga caacagagac ccaaaaatat ggaacgggtc
      721 gaaagttagt tcgtgatgtg tatgtattag acgggagtca attgctagga gggatcgaca
      781 accccaactt taagggcggg tttggtcaag ggcctgatcc taacatggcc cagggtcaaa
      841 atattatgaa tgacagcatg actggtgtgc ctgctggggc tgttcagcca ggatcagtag
      901 cttctggcat cgtgagtgag cttggtgtga tgaccggtgc tgctccttgc tggcaatgtg
      961 cccctcaacc tacaactgtc aatgaaaaga cggacaaaac cgttgcgact gactacggag
     1021 atgatcgcaa gaactataac aaggggactg ttcaataggt tcctggttat aaaatagttt
     1081 tgtgcttgaa aagagcgtgc cgggcaatat ccggcacgct cttttattat ctaggtaatc
     1141 attcgaagaa agtgcgattc tgttaataag ctgacggtgg gagaagattg agtgaacatg
     1201 gaagaattgg atcttcgagg tgtgatttgt ccgtacaatt tcgtgaagac gaagcttaag
     1261 ctggaatctt tagaagtcgg ttcccaattg agagtgttgt tggatgaagg cgaaccaatt
     1321 cgaaatgtcc ctcaaagtat taccaatgag ggtcaccaaa tccttaaaca ggaaaaagtt
     1381 gaacactatt accttgtggt tattcagaag gtaggtgtgt gatgggcgct cgagctagta
     1441 ctttcgggtt ctggttcaaa ttgcctttta cttcgttgag gtaccccttg aattaaaagc
     1501 gttatttctc cttttattga ttttgctttt aactgttgag tcacttcttc tatttcccct
     1561 cgcagtattt cttcgttgat ttttgtcagt tctcgtgtca atacgatgtt tctttttccc
     1621 atgacggtag tgatgacctg taatgtttta agaatccgat gctgggtttc aaagacaata
     1681 atggttcctt tctcttcttt aagattttga aaaaattttt cccttgcccc tggttttctt
     1741 ggaacaaatc cctcaaagat aaatcgatcc gttggtaggc ctgacacaca taatgccgta
     1801 agaacagagg aaggtcctgg aattggaata agtggaatgt ttgccttgat ggcctgacga
     1861 accagataaa aacctggatc agaaataaga ggggttccgg catcgcacac caaagcaacc
     1921 gaacttcctt cctggagacg atggaggaga atttcggttt tctcctcctt attgaaatcg
     1981 tggtaactgg tgagtggtgt gccgatatga taataggtgc agagtttttg tgtacgccga
     2041 gtgtcctcag ctgcaataat ggccacttct tttagaatgc ggatagcccg aaaggtcata
     2101 tcttccagat taccgatagg agtgctgaca atgtagagtg ttccggtcat ggtgtaggga
     2161 atatgatgta aggcaaggtt gagatggtac tgggtgagtc tgccattgtc cagaaaggtt
     2221 cagtcctact tgcctcgtcc ctttgtttca atatgaaatg gcatgaatgg atggataact
     2281 gacgaccata gattgcagga caggtagctt tgggttcggg atgtggatga tttgaaatat
     2341 caggatattc ttcgggtgat ttcaacctcc ttattatcaa atttcgttgt tacccatatg
     2401 ctcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaatga atttcgtgaa
     2521 atcgtgatta gtgcgattcc tataaataga atggcggaag acgagaatat tgcagcgtcg
     2581 tcataaattt ttgataggag gctagtgtga ttgggcaagc ttaaaacttt tcccagcact
     2641 gcataagtta tgttggggaa ttccggtttg tattaaatag gtgaaattgg taactcgttg
     2701 attttcttga ccttctatag agccagatct atactcgaca tgggtttctc tgcatgcttc
     2761 tttactagga tattcaatgc ctgaattgct gccatttctc accatgggct tttattttgg
     2821 aggcacgctc cttttccttt tatatctctg gcatcgttct gatgtcatcg ccaaaatttc
     2881 acttgtggcc acgggattag ggtttttctc tcatacttcg gcgcttatta tggctctggt
     2941 atcgacgggg catatcccta ttatgacctt taaggatgcg atgtcatttt ttgcctgggg
     3001 gttagtgctg gtttttttgg tggttggcct aaaaaggggt ttagcggtgt tgggcgcatt
     3061 tatccttccc ttggcatttc tgtctctcgt ttcggcaact cttgcgcctc agtcatctgg
     3121 cactcttgcc ccggtttttc agacggtatg ggtgcatgtg accctgagta ttttgggtac
     3181 catcggattt gctgtggcct tcgtggctgg gttgatgtat gtgatgcagg agcgcctttt
     3241 gaagtccaag caatttaatg tgttatcttt caagctcccg ccgctggatt ttttggattc
     3301 attaaatcaa cgatctatcc tttttgggtt tcccctatta accttgggaa tcctgacagg
     3361 ggcagtttcc gctcaattga cgattggttc atatttaagt tggaactctg agcaaatttg
     3421 ggcgcttatt acctggtttt tttatttcgc ggtcttgatg ggaagagtga cggccggatg
     3481 gagggcgaaa aaggccgcct atctaaccat tgtgggattt gccggagtaa ttttaacttt
     3541 tgttggcatc cttatcaaaa gtccgcaacc gatgtcaccg ctatgaatat tatcgtgtta
     3601 ggcctcaatc atcggacggc ttctgttgaa cttcgtgaac ttttggctat ccaggatagc
     3661 cggatgggag aggcgttggg acggctcatg gcttattcag gtattaaaga agccatgtat
     3721 ctggggacct gtaaccgggt tgaagtatat gccgtcgtgg aaaatggtga atggggcttt
     3781 cgtcaattag aagatttttt ggtctcaacc catttctcct tatcctccga ggatttactc
     3841 cctcatctgt atcgatacag tgaggatgaa gccatcgcgc atttgtttcg tgtgtcggcg
     3901 agtttagatt ccatggtcgt gggagaaccc caggtcctag ggcaggtcaa ggaagcctat
     3961 gagctggctt tgactcatcg ttcctccgga gtcattctta ataaggtcgt taagaaggcg
     4021 atttcggttg gcaaaagtgt tcggacgaat acgcggattg gggaatatgc ggtttccgtc
     4081 agctatgccg ctgtggaatt agccaaaaaa gttttttcga atctgaagca acgagtcgta
     4141 ttattagtgg gggcagggga gatgggaaaa ttggcggccc aacacctggt gaatcagggt
     4201 gtgaaggagg tcctcattac gaatcggaat catcatcgtg cttttgcact ggccgaacgc
     4261 ttccatggac aggcggttcc gtatgaacag cttcgaagca cgcttcccaa agtggatatt
     4321 gtgatttgcg ccaccggtgc tccccactat gtcattacca aagatgatat cgagatggcg
     4381 gtgtatcatc gaatgaaccg accaatgttt ttgattgata tcaccgttcc tcgaaatatt
     4441 gatccggcgg tcaaggatgt tgataacgcc tatgtgtttg aca
//
LOCUS       DAORAQ010000206         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2788_length_4466_cov_61.676944, whole genome shotgun sequence.
ACCESSION   DAORAQ010000206 DAORAQ010000000
VERSION     DAORAQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_4466_cov_61.676944"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /gene="ettA"
                     /locus_tag="PKM72_12715"
     CDS             complement(<1..1607)
                     /gene="ettA"
                     /locus_tag="PKM72_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNP61698.1"
                     /translation="MSTNDRQIIFSLVGVGRIHPPKKQVLKDIYLSFFYGAKIGVLGL
                     NGSGKSTLLRIIAGLDTNYIGNITFSKGYSIGLFEQEPYLEPGKTVKEVVEEGRAEVA
                     KLLRDYEEISQAFSGDVSPEEMEKLLERQGRLQEQIEAVNGWELEHQLEIAMDALRCP
                     PPDTKVDLLSGGERRRVALCRLLIQEPDILLLDEPTNHLDAETVGWLEQHLQEYKGTV
                     IAVTHDRYFLDNVAGWILELDRGQGIPFQGNYSSWLEQKQARLAQEEKSESKRQKTLE
                     RELEWIRMSPKGRQAKGKARVTKYEQLVSEEQSQQTDDLEIYIPSGPRLGDVVVEASN
                     LSKSYGDCLLFDGLSFSLPKGGIVGVIGPNGAGKSTLFKIIAGMVKPDTGDLILGETV
                     TLGYVDQNREIDGNLNVWECISDGKETVALGKMEVNSRGYVSRFNFSGSDQQKMVKDL
                     SGGERNRVHLARTLKEGANLLLLDEPTNDLDVNTLRALEEGLNHFGGCAVISSHDRWF
                     LDRVATHILAFEGNSQVSWYEGNYSEY"
     gene            1860..2489
                     /locus_tag="PKM72_12720"
     CDS             1860..2489
                     /locus_tag="PKM72_12720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNP61699.1"
                     /translation="MKHAQASRSTTCSSKFRSTTLPNNIKYCAQCGSVVEEKIPSGET
                     LLRAVCPSCHIIHYENPKIVAGSIPEWEDKILLCRRAIEPRLGLWTFPAGFMELGEST
                     EEAAARETLEEAHAEITIHSLLGIFSLPHVSQVYVVYRAHLQNLNFKPGPESLDVQLV
                     TLADIPWEHLAFPVIHESLTRYVKDIQTATAQTHSAVVPTKKPPSFYSS"
     gene            complement(2648..3151)
                     /locus_tag="PKM72_12725"
     CDS             complement(2648..3151)
                     /locus_tag="PKM72_12725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNP61700.1"
                     /translation="MTQKSSKELEVSYWISPSPVGRLLLVGSPYGLHALQFQDGAHPL
                     DILPTWKKSREPFHAALEQLKEYFDGSRTQFQIALNLQGTSFQRHVWKALQGIPYGRT
                     VSYGEIARQVGSPKASRAVGAANGQNPVSIIVPCHRVIGSSGKLVGYGGGLPIKTALL
                     ALEQLRH"
     gene            3260..3727
                     /locus_tag="PKM72_12730"
     CDS             3260..3727
                     /locus_tag="PKM72_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNP61701.1"
                     /translation="MSIHQHLGQWCQTRGLSLADLSQQTGVPLTTLQDFQNGNWDPPL
                     SIMTLIAQSLNVPDAWLHHDPRTLLRLWNDPDEDNPELPSLSSPDPLFQRLIQTSRDH
                     QDLFVLLTSLLHHGDPKLIRAAQVNLQSLFKQIRPTTVPWGSRPPGHFEPPND"
     gene            complement(3819..4187)
                     /locus_tag="PKM72_12735"
     CDS             complement(3819..4187)
                     /locus_tag="PKM72_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61702.1"
                     /translation="MNRILVIDDDIQVRESLSLSLESAGYTVMAAGTCQEGLQNHVNF
                     QASLIIMDVVAMEEREDEPTGLGSGPSPSIPMITLTGNIPTPGGTMKGPHQQFQPICT
                     LQKPFTVDELLQTVKRALVY"
BASE COUNT         1210 a         1218 c          932 g         1106 t
ORIGIN      
        1 tcatattcac tgtaattgcc ttcataccaa ctcacctggc tgttcccttc aaaggctaaa
       61 atatgggtgg ccacacgatc cagaaaccat cggtcatgac tgctgatcac cgcacacccc
      121 ccgaaatgat tcaagccctc ctctaaagcc cgcagagtat tgacatccag atcattcgtc
      181 ggctcatcca gtaataacaa gttcgcaccc tccttcaggg tgcgggctaa atgaacccgg
      241 ttgcgttctc caccggataa atccttaacc attttttgtt gatcagaccc ggaaaaatta
      301 aaccgtgaca catagccacg cgaattcact tccattttcc ctaatgcgac ggtttctttt
      361 ccatcagaaa tacattccca gacattcaga ttgccatcaa tctcgcgatt ctgatcgaca
      421 tagcccaggg taaccgtttc acccaaaatc agatcgcccg tatccggttt aaccatcccg
      481 gcaatgattt taaacagggt ggactttccc gccccattcg gcccaattac accaacgata
      541 ccgcctttgg gcaaagaaaa tgagagccca tcaaatagca gacaatcccc atagctcttt
      601 gagagattgg aggcttccac cacgacatca ccaaggcgtg ggccggaagg gatataaatc
      661 tccaaatcat cagtttgttg gctctgctct tcactcacca attgttcata cttcgtcaca
      721 cgggccttcc ccttagcctg acgacccttg ggagacatgc gaatccattc aagttcccgc
      781 tccagagttt tctgtcgttt cgactcggac ttttcttcct gggccagtct ggcctgtttc
      841 tgctctaacc atgaagaata gttgccctgg aacgggatac cttgcccacg gtccaactcg
      901 agaatccaac cagccacatt atctaaaaaa taccggtcat gggtcaccgc aatcaccgtg
      961 cccttatatt cctgaagatg ctgctccaac caccccactg tctccgcatc taaatgattg
     1021 gtgggctcgt caagcagcaa gatatcgggt tcttggatca gtaatcgaca caaagccact
     1081 ctgcgacgtt cgccgccgga caagaggtca actttagtat ccggtggtgg acagcggagg
     1141 gcatccatgg cgatctctaa ttgatgctcc aattcccacc cattgactgc ctcgatttgc
     1201 tcttgcagtc tcccctgccg ctccagtaac ttttccatct cctcaggact cacatctccc
     1261 gaaaaagctt gactgatctc ttcgtaatcc cggagcagct tggcaacctc ggcccgccct
     1321 tcctcaacga cttccttcac cgtttttcca ggctccaaat acggctcctg ctcgaaaagg
     1381 cctatggaat agcccttcga gaaggtaata ttcccgatat aattggtatc caagccggca
     1441 ataattctga ggagcgtact ttttcccgaa ccattcaatc ccaacactcc gattttggcc
     1501 ccgtaaaaaa atgagaggta gatatctttc aagacctgtt tcttcggagg atgaatgcgt
     1561 cccacaccca ccaatgaaaa aataatttgc cgatcattcg tactcatgcc attcgctcca
     1621 atcagttctc acgcgatcca aaatttgtca gggttgcaag aagacacatg cagaggccaa
     1681 ccttaccaaa ggaatacgag gattcacaat ccaggctggg aagtgagccc cttcccttca
     1741 aaacgtgact gagatatgct ccgcataatg gaaagggctc attgtaaagc acggcattga
     1801 attacaggcg agacagagta tgataaggaa agacccacgc aacccaggtc tttcagatcg
     1861 tgaaacacgc tcaagcatct cgctcaacga cctgttcatc taaatttcga tctaccacct
     1921 tgcccaacaa catcaaatac tgtgctcaat gcggatccgt cgttgaagaa aaaattcctt
     1981 caggcgaaac ccttctcaga gccgtctgtc catcctgcca tatcatccac tatgaaaacc
     2041 caaaaatcgt ggcgggcagt attcctgaat gggaagataa aattctttta tgccggcggg
     2101 ccattgagcc acgattaggg ctctggactt ttccggctgg atttatggaa ttaggcgaga
     2161 gcaccgaaga ggcggcagcc cgggaaaccc tggaggaagc gcatgcggaa atcaccattc
     2221 atagcttatt gggtattttt agccttccac atgtttctca ggtctacgtg gtctaccggg
     2281 cacatttaca aaacctgaac tttaaacccg gcccggaaag cttggatgtc caactggtca
     2341 ccttggccga catcccctgg gagcatctgg cctttcctgt catccatgaa tcgttaactc
     2401 gatacgtcaa ggatatccaa acagccactg cgcaaaccca ttccgccgtc gtccccacca
     2461 aaaagcctcc gtcattttat tcgtcctaac cgtcgaccgg cagtgaagtg gttcaatcaa
     2521 gataaatggt taaaaaaaag attcatagga ataattgtgg acaataaaat ggggcaaagg
     2581 attagtttac ggtcgaagct actattccag acgtgaggta aggggcctgg cgaaaatgac
     2641 aagcgtttta atgcctcaac tgttctaatg ccagaagtgc ggtttttatg ggtaacccgc
     2701 cgccgtagcc caccagcttg ccactagatc cgatcacccg gtggcatggc acgataatag
     2761 agacagggtt ttggccatta gcagccccca cggcccgtga agctttcggg ctgcccacct
     2821 gcctggcaat ttcaccatac gacaccgtgc gaccataggg aatgccttgg agagctttcc
     2881 acacatgtcg ttggaaggag gtcccttgaa ggttcaatgc tatctgaaac tgtgttcggg
     2941 aaccgtcaaa gtattccttc agttgttcca atgcggcatg aaatggctca cgcgattttt
     3001 tccaggtcgg caggatgtcg agggggtggg ctccatcttg aaactgcaga gcatgcaacc
     3061 catatgggct tcccaccaat aaaagccggc caaccggact tggactaatc cagtaggata
     3121 cctctaattc cttggatgac ttttgagtca ctggtggctt cctcatagtg agtttcacga
     3181 ttgtagaaag tgaagcacgt tcgggtttgg taaaaattga ggatggacag gctaatcaat
     3241 attttcctat gatcgaatca tgtccattca tcaacatttg gggcagtggt gtcaaacaag
     3301 aggactctct ctggccgacc ttagccaaca aacgggagtg ccactcacga ccttacagga
     3361 tttccaaaat ggaaactggg acccaccgtt atccattatg accttgattg cccaatcgtt
     3421 gaatgtgcct gatgcctggc tccatcatga tccccggacc cttctgcgat tatggaatga
     3481 cccggacgag gataatcctg aacttccgag cctgtcttct cccgatcccc tgtttcaacg
     3541 actgatccaa acaagccgtg accaccagga cctcttcgtc ctactcacca gcctgcttca
     3601 ccacggtgat cctaaattaa tccgcgcggc tcaggttaat cttcaaagtc tcttcaaaca
     3661 gatacgcccc accactgtcc catgggggtc gagacctcca ggacatttcg aacctcccaa
     3721 tgattaacca cccaacctca tcttactcaa tgattgagtt gagccaaagg gagaaatgac
     3781 tgttatgaaa atggagcaga aacaggagaa gtaccgactt aatataccaa agcccttttc
     3841 acggtttgga gaagctcatc tacagtaaag ggtttttgca aggtacagat cggctggaat
     3901 tgttgatgag ggcccttcat cgttccgccc ggtgtgggaa tattgcccgt caaagtaatc
     3961 atcggaatac ttggagatgg tccggaacca agccctgtgg gttcgtcttc cctctcttcc
     4021 atggccacca catccataat aatcagggac gcttgaaaat ttacatgatt ttgaagacct
     4081 tcctgacagg tccctgcggc cataactgta tacccggctg attccaaaga aagagaaaga
     4141 gactctctca cctgaatatc atcatcaatc accaaaattc tattcattga gttaaccata
     4201 agttattcac aaataaaatg aaaataaccg ttaccataga acaaaacgca actttctcct
     4261 ccggtaaacg agcaatttac gttccttctc aattgaaatg gcctaatgta attattcact
     4321 cataaaaaca aatacttacg aattaaactc ggaaatattt tcgtttgcaa tatgaaggtc
     4381 taaacattca atatgcaggg attagcattc aatgatcccc atccactccc taatgaacct
     4441 tggaataacc tgacgggacc tagaac
//
LOCUS       DAORAQ010000207         4444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2805_length_4444_cov_66.052632, whole genome shotgun sequence.
ACCESSION   DAORAQ010000207 DAORAQ010000000
VERSION     DAORAQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_4444_cov_66.052632"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /gene="modC"
                     /locus_tag="PKM72_12740"
     CDS             complement(<1..833)
                     /gene="modC"
                     /locus_tag="PKM72_12740"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNP61703.1"
                     /translation="MSELTARFEVAFPSFHLQVKASVPAEGITVVFGPSGCGKTTFLR
                     CLAGLERSPTGFLALGEHVWQDESNNNFLPLSQRPVGYVFQEPRLFPHLSVRSNLLYG
                     WKRIPESQRRISLDQVVEILGLDSLLERRPTHLSGGEQQRVAIGRALLTSPRLLLMDE
                     PLSSLDVQRKREIITFIQRLDKEFRIPIIYVSHALHEVVQLARTLILLKEGSVAAIGP
                     LAEVFSQVGSRRVIPDSHIGAVIDTRVAEHDVEFGLTTLNFSGGQLSVPHQHSSIGES
                     LR"
     gene            complement(830..1513)
                     /gene="modB"
                     /locus_tag="PKM72_12745"
     CDS             complement(830..1513)
                     /gene="modB"
                     /locus_tag="PKM72_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNP61704.1"
                     /translation="MTLSEVDLGPLWLTLRLAGVTVIVLLIIGTPLAWWLAHTRSRAR
                     TAIEAIVAMPLVLPPTVLGFYLLVLLGPHGWIGSASQAVTGSALSFTFTGLVIASALY
                     SLPFVVQPLHGAFESIGRKPLEAAWSLRASKLDTFLTIVSPMASRGYLTAIVLGFAHT
                     LGEFGVVLMVGGNIPGRTKVLSIAIYDHVEVLEYTQAHVLSAGLLVFSFLVLLMVYTV
                     NRRLPIHVS"
     gene            complement(1581..2345)
                     /gene="modA"
                     /locus_tag="PKM72_12750"
     CDS             complement(1581..2345)
                     /gene="modA"
                     /locus_tag="PKM72_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNP61705.1"
                     /translation="MMFLRVHTFIFMFFFYVCVTTAAAEEVRVAVAANFLATLNEIIT
                     SFQKDMGHTILVSSGSSGKLYAQIKNGAPFDVLFSADAERPQLLEREGLAVKGSRFVY
                     SVGRLTLWSSDSNLVQGEAHHLLSGGHFDHLAIANPKTAPYGRAAEQTLKKMGLWDSL
                     KARIVQGENIGQAFQFVYSKNAQLGFVALSQVVDPKINGSGSRWDVPTSFHDSLEQEA
                     VLLTTGQNHAGARAFLDYVKGEKSRAIIERFGYGLP"
     gene            complement(2426..3946)
                     /locus_tag="PKM72_12755"
     CDS             complement(2426..3946)
                     /locus_tag="PKM72_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNP61706.1"
                     /translation="MEKGASHVKVWMLTALGMALVISCTVVLAAEPTTSHPTSIASEK
                     GEAFGDFFKEVKERAETQKGKNWKETADASLIQDKETQWDRYLKAAMGLPDFVDLGIE
                     NRTRFESVSHPWRSTAKIGGGRTDSQLALRTRVRFGLGNGPLRFLFEGQDSREYGAQT
                     GGFVNNTTVDEWDILQLFGSLTVDDVLGSGLRTDLHFGRMTLDLGSRRYVARNDFRNT
                     QNAFDGLHWQIGKADTWRFRTFITEPVIREEVKLDEQNKKFLFWGAYVENLQISWMNI
                     NIFYYGLNDQRQQNKNFHRTYGTYGFRLFKDSAIGEFDYELEGAVQVGQLGQVDHFAY
                     NPNAQVGYTFNVPWTPQFLVQYTYASGTRTPGGSQNGTFDPLFGARRWDLMATGIFGP
                     FLRSNISSPGWRLVAKPVNNVTMQIKQRFWYLAQGGAVNGGILLQDPSGGAGNYLGHD
                     IELRATWTVSQNLEFDAGYDHWFKGSYFKRLPASANLPQGGDKDTDYFYLSMRIRL"
BASE COUNT         1187 a         1163 c         1054 g         1040 t
ORIGIN      
        1 cgtaatgatt ctcctatgga tgaatgttga tggggcactg acaattgacc gccggaaaaa
       61 ttgagagtgg taagcccgaa ttccacatca tgctccgcta cacgagtgtc aatgactgca
      121 ccaatgtggc tgtccgggat gacgcgacga ctgcctactt gagaaaacac ttcagcaaga
      181 ggtccgattg cggccacgct cccctccttc agcaggatga gagtcctggc gagttgcacc
      241 acttcatgca gcgcatgact cacatagata atgggtatcc tgaattcctt atccagccgt
      301 tggataaatg ttatgatttc ccgctttcgt tgaacatcaa gggagctgag cggttcgtcc
      361 atgaggagca ggcgcgggct ggttaataaa gcgcggccaa tggccactcg ttgctgttct
      421 ccgccagaca gatgggttgg cctgcgttcc aacaacgagt ctaaacccag gatctcgacg
      481 acctgatcga gtgaaatacg acgctgcgat tccggtatcc gtttccatcc gtacagcaag
      541 tttgatcgaa cgctcaaatg agggaacaag cgaggctctt gaaagacgta accaaccgga
      601 cgttgggaaa gtggaagaaa attattgttg gactcatcct gccagacgtg ctctcccaag
      661 gcgagaaagc cggtcggcga tcgttccaat ccggcgagac agcgcaagaa ggtggtctta
      721 ccgcatccgg aaggaccgaa tacgaccgtg atcccctcgg cggggacact ggccttcacc
      781 tgcaggtgga atgagggaaa agctacctcg aatctggctg tcaactcact catgagacgt
      841 ggataggcaa gcggcggttc accgtataga ccataagaag gaccagaaat gaaaagacaa
      901 gaagccctgc ggacaggaca tgagcctggg tatattccaa cacttccaca tggtcgtata
      961 tggcaatgga gagtaccttc gttctccctg gaatattccc tcccaccatc aagaccacac
     1021 caaactcgcc aagggtatgt gcgaatccaa ggacaatcgc cgtgagatag ccgcgagagg
     1081 ccatggggga aacaatggtc agaaatgtgt cgagctttga ggctctgaga gaccaggccg
     1141 cctctaacgg ctttctcccg attgactcaa aggcaccatg caagggttga acgacgaaag
     1201 ggagcgaata caaggcagaa gcaatgacga gtccggtaaa ggtgaacgaa agcgccgatc
     1261 cggttaccgc ctgcgaggcg ctcccgatcc atccatgcgg acccaataag acaagtaaat
     1321 aaaatccgag cacggtgggg ggcaatacga gaggcatggc gacaattgcc tcaatggccg
     1381 tcctggctcg ggatcgggta tgtgcaagcc accaggcaag cggcgttcca atgatcagaa
     1441 gcacgatcac cgtcactccg gccaggcgca aggtcaacca gagaggtcct aaatccacct
     1501 cagataatgt catggcttcc tatgtagcat tatcaccttt ggaatggaag atcttttctc
     1561 attggtcgat atgtggtcga ttatggcagt ccatagccaa atcgctcaat aatggcccgg
     1621 cttttctccc ctttgacata atccaaaaag gctctggcac ctgcatgatt ttgtccagtt
     1681 gttaacagga ccgcttcctg ttcgagtgaa tcgtgaaaag aagttggcac atcccaccgg
     1741 ctgccgcttc cgttgatttt tggatccact acttgggaaa gggcgacgaa accaagttgg
     1801 gcgttctttg aatacacgaa ttgaaaagcc tgtccgatgt tttccccctg aacgatccga
     1861 gccttgaggg aatcccagag gcccatcttc ttgagtgttt gttccgcagc cctgccatag
     1921 ggcgccgtct tcggattggc aatggccaga tgatcgaagt gcccacctga gaggagatga
     1981 tgtgcctccc cttgaaccag gttcgaatcc gaactccata aagtcagacg ccccacagag
     2041 taaacaaatc ggcttccctt aacggccaac ccttctcgtt ccaacaattg tggacgctcg
     2101 gcatctgctg aaaacaagac atcgaacggt gcaccgtttt tgatttgtgc atagagtttt
     2161 ccgctcgatc cggaactgac cagtatcgtg tgccccatgt ctttctgaaa actcgtgata
     2221 atttcgttaa gagtcgccaa aaaattcgcg gcaacggcca ccctgacttc ctcggcggcg
     2281 gccgtcgtga cacaaacata aaaaaagaac atgaagataa aagtatggac tcggagaaac
     2341 atcatacacc tcccaattca atagatgtcg ccaagtggct cagtctagag aacaggctga
     2401 ccctgattaa gggtttagag ccgtttcaca atctgatgcg catggaaaga taaaaataat
     2461 ccgtatcctt gtccccgcct tgaggcagat tcgccgaagc gggcagccgc ttaaaatagg
     2521 atcctttaaa ccaatgatcg tacccggcat caaattcaag gttttggctg acggtccacg
     2581 tcgcacgtaa ttcaatatca tgccccaaat agtttcctgc ccctcctgag ggatcttgta
     2641 aaaggatccc cccattcacg gcaccgcctt gagccaaata ccagaatcgc tgcttgattt
     2701 gcatggttac gttattgaca ggcttagcaa ccaacctcca accgggggaa ctgatgttgg
     2761 accggagaaa tggtccgaaa attcccgtcg ccatcaaatc ccaacgccga gccccaaaca
     2821 gaggatcaaa cgttccgttc tgacttcctc ccggcgtgcg agttccgctg gcataggtat
     2881 attgcaccaa aaattgagga gtccagggca cattaaacgt atatccaact tgggcattgg
     2941 gattataggc aaaatgatcg acctgcccta attgtcccac ttgaacggcc ccctctaatt
     3001 cataatcgaa ttcaccaatg gcagaatcct tgaataggcg aaatccatac gtcccatacg
     3061 tacgatgaaa attcttattt tgttgtcgtt gatcgtttaa tccgtaataa aagatattga
     3121 tattcatcca ggagatctga aggttttcca catatgcgcc ccaaaataaa aattttttat
     3181 tctgctcgtc gagtttgacc tcctcacgaa taaccggttc ggtaataaag gtgcggaatc
     3241 gccaagtatc ggctttccca atttgccaat gcaggccgtc gaaggcattc tgggtatttc
     3301 ggaagtcatt gcgggcgaca taccgacggc ttcccaaatc cagggtcatg cggccgaaat
     3361 gcaagtccgt tcgcaatccg cttcctaaca catcgtccac ggtcaatgac ccgaacaatt
     3421 gtaaaatgtc ccattcatcg accgtggtgt tattcacaaa gcctccagtc tgcgctccgt
     3481 actcacgaga atcctgacct tcaaagagaa accggagagg accatttccc agtccaaagc
     3541 gcaccctagt cctgagggcc agctgggaat ccgttcgtcc ccccccaatt tttgcagtgg
     3601 atcgccaggg atgactgaca ctttcgaatc gggtccggtt ttcaattccc aaatcaacaa
     3661 aatccggcag gcccatggcc gccttcagat accgatccca ttgagtctct ttgtcctgga
     3721 tgagcgaagc atcagccgtt tccttccagt tttttccttt ctgtgtttct gctcgttctt
     3781 taacctcttt aaaaaaatcc ccaaacgctt cccctttttc actagctatg gaagtgggat
     3841 gtgaagtcgt cggttccgcc gccaaaacca ccgtgcaaga aatcaccaag gccattccta
     3901 atgcagtcaa catccagacc ttcacgtgac tcgccccttt ctccatacat tccacattcc
     3961 cttcatgcat cggaatgacc agaaagatga gacggttctt cgcatgaaat tcagcataat
     4021 gtaattttta aacatacaaa tgtcaattat aaattacgct tatagaatga ccagggttag
     4081 ctcgggtgtt tggatttcca tacgaaacag aacaacaaaa ttccagatgg accgatgatt
     4141 ggggaaacct gctttttccc aagatatcct tctcacaaga attcaaagtt gggcgaaaag
     4201 atggcatgcc atgcgcacgg cgggaggatt tcacattagg cgtaagactt atggaaaggg
     4261 gacaggagta gtatagtacg aaaatcggaa atgtcacttc tcaggattcg caacggactt
     4321 ttctggcgat ggattcgata tcttttccag gttcaggatg accagatgag tccagtcttt
     4381 aggtctgggc tccggtcggc aaatattgac ccagcacaat ctcactgact ttcttattcc
     4441 agaa
//
LOCUS       DAORAQ010000208         4382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2847_length_4382_cov_66.192281, whole genome shotgun sequence.
ACCESSION   DAORAQ010000208 DAORAQ010000000
VERSION     DAORAQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_4382_cov_66.192281"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKM72_12760"
     CDS             complement(<1..593)
                     /locus_tag="PKM72_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNP61707.1"
                     /translation="MEKLENTRNQKNQLVSIVIPVYNERKTIETIVCRVQNVKISKEL
                     IIVDDGSTDGTREWLIEQFGQGTSDEEASQKELGESGADSSGVRVLTHQRNQGKGAAL
                     KTGFLCAKGSIVLVQDADLEYSPDDYHRLLEPVMTGRADVVYGSRFLGARKGNWLLVS
                     YLGNKIFTALTNAATGLSLTDVWTGYKVFKREVLTDIS"
     gene            1010..1441
                     /locus_tag="PKM72_12765"
     CDS             1010..1441
                     /locus_tag="PKM72_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61708.1"
                     /translation="MMNITISSPQTLEQVSGTVSVTILLIDDSPDNLMILGDILERIG
                     YQIVCAEDGESGLEQAKRVQPDLIFMDVKMPGLDGFQVCEQLRAQKSFQRTPIILMST
                     QSDSTNSELALRAGASEFWPKPISPGKILNKLAGFLRNGPE"
     gene            3423..3638
                     /locus_tag="PKM72_12770"
     CDS             3423..3638
                     /locus_tag="PKM72_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61709.1"
                     /translation="MNATMTIGRSREEGMFLLRESDYVWRVHEDGKVDCALHGDATAF
                     ERLSFIQKWRTPSRRIFQEYPFQDFSH"
     gene            complement(3919..>4382)
                     /locus_tag="PKM72_12775"
     CDS             complement(3919..>4382)
                     /locus_tag="PKM72_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNP61710.1"
                     /translation="SSFNDVSASHWRRTGASGEYSSHDRVRVRNSANTGTLTGITIAG
                     EPGYRVNRGSVVRPEFTIENNGKQTHANVSFGLYVSTNDFISYSDTRIGGGSFGSIHP
                     ADVFTTTIPVIIPNFLNAGQNYWLGIIVDEDNDINEVNGSNNRAYIPIRVQ"
BASE COUNT         1216 a         1099 c          859 g         1208 t
ORIGIN      
        1 gagatatctg ttaagacttc ccgtttaaac actttgtacc cagtccatac atccgtcaag
       61 gataaacccg tcgcagcatt ggttaacgca gtaaaaatct tgttacccag atatgagacg
      121 agtaaccaat tccctttccg tgcccccaaa aatctggacc catagacgac atcagcccga
      181 ccggtcatca ctggctccaa cagccgatga taatcatcag gactatattc caaatccgcg
      241 tcctgcacga ggacaatgct ccccttggca cacagaaatc ccgttttcaa ggccgcgccc
      301 ttcccttgat tgcgttggtg agtcagcact cggacaccag aactatcagc accagattca
      361 cccaattctt tctggcttgc ttcttcgtcc gaagtcccct gaccgaactg ctcgatgagc
      421 cattcgcgag ttccatctgt ggatccatca tccacaatga tcagttcttt tgaaattttc
      481 acattttgca cacggcatac aatcgtctca attgttttcc gctcgttgta tacgggaatg
      541 acaatactta ccaattgatt tttttgattt cttgtatttt ccagcttttc catgtcaatt
      601 tcgattaacc aattatgata aagagatttc aggtagacta ccatgaacgt tcagacaacc
      661 ctcatcaaat atcatttttt ggtaaagccc accccactcg tggtctctga tatcggccta
      721 tgatatctcc gtatcaaatg ccccagtgac caccaggaac agacggatat cacccatttg
      781 tgaatggttt tctcctggac gtcccaaatt tgctttgtct caaacccatt agctgtccca
      841 tcctatttgt ctcatggaac acctcacaat acccgttcca acaagaaaaa ttctataaaa
      901 atagttatat ttcaaatagt tatacaacaa aatcaacata gcactatcct tgcttattac
      961 atgggtaaca aaagtagatg aggcaatacg tcaaagattg gagggaggca tgatgaacat
     1021 aacaatatct tcaccacaga cattagaaca ggtatccggc accgtttccg tcaccatact
     1081 tctcattgat gattcccccg ataatttgat gattctcggg gatattttag aacgaattgg
     1141 ctatcagatt gtgtgtgcag aggatggcga gagcggcctc gaacaggcga aacgcgttca
     1201 acccgacttg atatttatgg atgtgaaaat gcccggcctt gatggattcc aagtctgtga
     1261 acaacttcgc gcacagaaga gtttccaaag aactccgatt atcctcatga gcacacaatc
     1321 cgatagcaca aacagcgagc ttgccttgag ggctggtgcc tctgaatttt ggccaaagcc
     1381 catttccccg ggaaagattc tgaataaact agctggtttt ttgcgaaacg gtccagagta
     1441 acgaggctcc ttttccatcc tttacgcact cctcctattc agccccaact tacattcttt
     1501 tgatggtact catatcccac atgaggtcta aggatacata aaatcctgaa ttgtctggca
     1561 tataaattgc tgtttaattt aaaatggtgt tttttcctta aaaacatcct attttagata
     1621 cattttgaag ggaaaatcac acagacagag tcatttacca aacgactatt tatcctattc
     1681 acctttcaca acatggagaa agtacgcatg gtcacccgtc taagaaaaaa ttccaccatc
     1741 aatctgtcca catggggcca acgaggaacg gcaggattgg tcgaggaatg ggaaccgcga
     1801 atacccattc cggaagaaaa gttacaatcc cgtcgagggt caacgatctc aattccgaaa
     1861 tcaaccgagc cgcccacaga agagaaaaat ctaaaaaaat aagtactttc ccaattccct
     1921 tcgtgactcg tgtatccagc caccaccccc atggactggt tttctgataa taaattttaa
     1981 accttcaacc cgaatagcat tcggttcacc cccatcgtaa gagtccacca catcccagac
     2041 aatatttctc tggagtcctg aaacaatttc gtttacccca aacaacgcag aacatcaatt
     2101 gaccgcattg cccttactgg tctgctccgg aaaaagaacg taattacctg acagccatat
     2161 tgggattaaa gaccggcttc caagaattcc ccggtcacat ccatattcca aagaaaatcc
     2221 ccttgcctcg gtttgcccta cctttttaag ttcccgaaag tagagcattg aaattctact
     2281 tttatagctt acaggtgcag acatgaagag actctccctg gccatgcatt gtttggtgtg
     2341 aagtcctata ggattgtttc ctgccctaga gagaggaatt taggttgtat ttaaaggtga
     2401 tagctggctt ttggagtaat ccgagatcgc ggcctcgccc tacgaaatca ctgaatgacc
     2461 tataggacga ccaacgctaa ttaaacaaaa gggattttat attttcagac cgttgaaaga
     2521 agtatggtct agcgctcgca gttcgacttg accggcgggt caggacttac cgggaggatt
     2581 aaaggatttt gttcgatcaa gtctatttgt tgatggtggg gcgtttcttt cccagacaaa
     2641 attgtttccc cgtccaacaa caaactcgat attctaaaat ccgtaattcc aaatcctgaa
     2701 tttcggaaat tctgcagatg gacatttaga aaaaccacgc tattgctata cagcaaattc
     2761 ttcacatatt ttcgttcaat gtcggaccgg cacaatgctc ccttcctcat cattgcatta
     2821 ggaccagtca atatattttt acctgccacc tatgtttacg gcatgtaccc cttctggtac
     2881 ttccccacat cacacaggac aaaggttcct agaatatacg attttctcct ctttgttttt
     2941 cccctttaca attagttatt tagcttgcat tcctggttgt atagaagcca aaaacgaccg
     3001 acggcatcaa ggttgctttt attgtgaacc aggaacagat gacagacgac tgaataaaaa
     3061 gagaggcaac accatggaag gtattttact catcatcatc agctgtgtaa gtctgttggg
     3121 actggtttct ttgttttggt tatggcgacc ctaggcatga actctaaatt ggggaagacc
     3181 tccatatcat gccttcctta acaactttca tgctcgcgat tgatacagtt aattgaagtg
     3241 aattattgcc cacctatacc tatagatttt atgctgcagg atatttgttt tgcgttcgat
     3301 gagaacattt tatccagctg gtgatttttt acgaacgaag aagtccgttg ttcggaatgc
     3361 accaacgtgt taacaaattc tgtgagcagc aacgcctcaa tgtccaaaag gagaggcaag
     3421 aggtgaacgc caccatgaca atcggtcgat cacgtgagga gggaatgttt ctacttcgag
     3481 aaagcgatta tgtgtggaga gtgcacgaag acgggaaggt ggactgtgcc ctccacggag
     3541 atgccaccgc attcgaacgt cttagcttca ttcagaaatg gagaacccca tcccgtcgga
     3601 tttttcaaga ataccccttt caggattttt ctcactgact gacaggactt ttatgccaga
     3661 actctaattt ttatcatcct gctttagact gcctgattct acgggcacat gattaacaac
     3721 gatccctttg gacaagtcga cgtgaacatt cttgaaaagc cgatgccgtg caagattcct
     3781 ccatgaatcc tgacggcatc cgctaccttg acctcccatt gaccaggccc ggggatccgg
     3841 ccaagactca ttccattttt gagccaaaaa ccacaggcgt tcttgtacct gcatccctca
     3901 cttgcatctt cacctttttc actggacccg gatgggaata taagcccgat tgttcgaacc
     3961 atttacctca ttgatatcgt tatcctcatc aacgatgatt cccagccagt aattttgccc
     4021 cgcattcaga aaattgggga tgatcaccgg aatcgtggtg gtgaaaacat cggcagggtg
     4081 aatcgaccca aagcttccac caccgatacg ggtatcgcta tagctaataa aatcattggt
     4141 tgagacatag agtccaaaac tcacgttggc atgagtctgt ttcccattat tttcgatcgt
     4201 aaattcaggc cgtaccacac ttccccggtt tactcgatac cccggctccc cggcgatcgt
     4261 aatacccgtc aacgtccctg tgttggcact attccggacc cgtacccggt catgacttga
     4321 atactcaccg ctggctccgg ttcttcgcca gtgcgaggcg gacacatcgt taaaggagga
     4381 gc
//
LOCUS       DAORAQ010000209         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2850_length_4378_cov_72.645385, whole genome shotgun sequence.
ACCESSION   DAORAQ010000209 DAORAQ010000000
VERSION     DAORAQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2850_length_4378_cov_72.645385"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..979)
                     /gene="sppA"
                     /locus_tag="PKM72_12780"
     CDS             complement(140..979)
                     /gene="sppA"
                     /locus_tag="PKM72_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNP61711.1"
                     /translation="MILGGLVLFSIGKSLIPELLLAGKDGVAIVRVEGPILDSFQTVE
                     ELKKFADDPLVKAIVVRIDSPGGGVAPSQEIYNAVKRVRKEKNKTVIASMGTVAASGG
                     YYIAVATDRILANPGTLTGSIGVIMQLANFHELLEKIGVKNFVVKSGKYKDIGSPFRP
                     MNDEDRKVLELVMNDVHRQFIDAVAEGRSLDVAEVEQLADGRVFTGQQAKSILLVDDI
                     GDLHDAIKLAGELGGIEGEPRVVEVSKPFSFRDLLENTFLGSVRTFATTHFSTPLMYL
                     WVS"
     gene            complement(1082..2785)
                     /locus_tag="PKM72_12785"
     CDS             complement(1082..2785)
                     /locus_tag="PKM72_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNP61712.1"
                     /translation="MNTLTQQPEKMDRETLAALYDETFKNIEEGTITEGRVIDIQRDR
                     VVVDIGYKSEGMIPADQFSPEELEQLAVNDPVQVYIEQCEDADGNLLLSKEKADKMKV
                     WEDLEVAHKDEKQVQGKVISRIKGGMIVDIGVKAFLPGSQIDLTPVRDLDGLVGKTYN
                     FKIIKINHRRGNVVVSRRALLEETRDKRRQSTLSTLSEGQLIEGTVKNITDYGAFLDL
                     GGIDGLLHITDISWGRVGHPSDMFSIGDRVEVLVLKYDRETGRISLGVKQKSPDPWTK
                     VEEKYPSRSRVNGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSKVVSVGDT
                     IEAQVLHVDQHSRKISLGMKQTAPNPWDIIEDKYPEGTEIEGKVKSVTDFGAFVGLDE
                     GVDGLIHISDMSWTKHIKHPSELFKKGQSVKAIILKIDKEKERLSLGYKQLISDPWVS
                     DIPQQYHVGEVTSGKVSKIADFGVFVELDGGVEGLIHISEIGPDVQERLEEKFAVGQD
                     VSAKIVKVDCDDRKIALSLWEHLKDLEQSEVEEFNSSQGSVDQSFGRVVQKKDQGDEG
                     E"
     gene            complement(2846..3511)
                     /locus_tag="PKM72_12790"
     CDS             complement(2846..3511)
                     /locus_tag="PKM72_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNP61713.1"
                     /translation="MSGAAYGLLWILFNGLARLLFRFRTEGLQHFPRTGGIIVAANHA
                     SYLDIPLLGCAIPRRVFFLGRASLFPNRYMNWALQKLGWIPLKTHKLDRKAFGQALSH
                     LQAGEPVVIFPEGTRTEDGALQIGKPGLGYLVAESNCQVIPVYISGTFTVLPIRARWP
                     RLFPLRVSFGKPLKFCKKDDGESAKSFYEDVSVTVMDHIAQLGGVPSPTRQAYETDLR
                     SKL"
     gene            complement(3508..4167)
                     /gene="cmk"
                     /locus_tag="PKM72_12795"
     CDS             complement(3508..4167)
                     /gene="cmk"
                     /locus_tag="PKM72_12795"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNP61714.1"
                     /translation="MITIDGPAGVGKSTVAKCLATRLGYVYLDTGALYRAIAWKVQED
                     QIDPNNTAHIQGLLARTDLRLMLSGDGLSVWVDGQAINQEAIRTPAISQLASCVAAIR
                     SVRDWLLPIQRKIGGEVGVIAEGRDMGTRVFPSADVKFFLDAELEVRANRRQQELGQQ
                     GKAVDLETVRDAMAVRDDRDRTRTVDPLRPAPEAIMINTSRQSVEEVVGRMMQIIVDR
                     L"
BASE COUNT         1148 a         1247 c          840 g         1143 t
ORIGIN      
        1 cttttccgcc aacttttcga tgagatccgc ttttgtcatc cgatcctcct tctcagaaag
       61 actcgctttt caacacttga ttcttgcgaa taagtataac tcctcaattg atgtacttga
      121 ttcatcttgc ttgtccggat caggaaaccc aaaggtacat caaaggggtg gaaaaatgag
      181 tcgtggcaaa ggtgcgaacg cttccaagga atgtgttttc aagtaaatcc ctgaatgaaa
      241 acggcttgct cacctccaca acccgtggct ccccctcaat cccgcctaat tcgccagcta
      301 acttaatggc atcgtgcaga tcgccaatat catcaactaa taagatggac ttagcctgtt
      361 gacccgtaaa gactcgccca tcggctaact gctcaacttc cgccacatcc agagaccgcc
      421 cctcggccac agcatcaata aattgacgat ggacatcatt catcaccaac tccaatacct
      481 tgcgatcttc atcattcatg ggccgaaaag gagagccaat atctttatat ttcccacttt
      541 tgactacaaa atttttgacg ccgatttttt caagcaactc gtggaaattc gccaattgca
      601 taatgacgcc aatgcttccc gttaaagtcc cagggttagc cagaatgcga tcggtggcca
      661 ccgcaatgta atagcctccg gaagccgcca ccgtgcccat ggaggcgatc accgttttat
      721 tcttttcttt tcttactctc ttgacggcat tgtaaatttc ttgggaaggc gccacgcccc
      781 caccaggact atcaattctc accacgatcg ctttgaccaa aggatcgtca gcaaatttct
      841 tcaattcctc gacagtctga aacgaatcaa gaatgggacc ctcaacccga acaatggcca
      901 caccgtcctt acctgctaac aataactcag gaattaggct tttcccaata ctaaacagga
      961 caaggcctcc gagaatcatc cacagaaccc gtttccatcg tggataccca ttactcatag
     1021 tattcgggat tggccatcct gaagaagccc catattcatc caatcattct gcccatgatg
     1081 cctattcgcc ctcatcccct tgatctttct tttggacaac gcgcccaaag ctctggtcca
     1141 cactaccttg agagctatta aactcttcaa cctcactttg ctccagatcc ttcaaatgct
     1201 cccaaagact taacgcaatt ttccgatcat cacagtcaac cttgacgatt tttgcggaga
     1261 catcctgacc aacagcaaac ttttcctcga gtcgctcctg cacatccggc ccgatctcac
     1321 tgatatgaat taacccttct accccgccat ctaattccac gaatacacca aagtcagcga
     1381 tttttgaaac tttgccggaa gtgacttccc caacatgata ttgctgagga atatcagaga
     1441 cccatgggtc cgagatcagc tgcttatacc ccaaggataa ccgctctttt tctttatcaa
     1501 ttttcaaaat aatggcttta accgattgtc ccttcttgaa cagttccgaa ggatgtttga
     1561 tatgcttcgt ccacgacata tcagaaatat ggatcaggcc atcgacaccc tcgtccaaac
     1621 ctacaaaggc accaaagtcg gtcacgcttt ttactttacc ctcaatctct gtaccttccg
     1681 ggtacttgtc ctcaatgata tcccaagggt taggagccgt ttgcttcatt ccaagagaaa
     1741 ttttccggct atgttggtca acatgaagca cttgagcctc aattgtatcg cccacggaga
     1801 ccacctttga gggatggcga acttcatgag tccaagacat ttccgacaca tggaccaaac
     1861 cttcaacccc tggctccaac tcgacgaatg ccccataatc ggtcaggctc acaaccctac
     1921 cattcacgcg acttcttgac ggatactttt cttcaacttt agtccagggg tctggagatt
     1981 tctgcttaac acccagggag atacgacccg tttcccggtc gtatttcaat actaagactt
     2041 caacacgatc tccaatggag aacatgtctg agggatgccc cacacggccc caggaaatat
     2101 cggtgatatg cagcagacca tcgatgccac ccaaatccaa gaaagcccca taatccgtta
     2161 tgttcttcac ggtcccttca atcaactgcc cttcagacaa cgtagacaaa gtcgattgtc
     2221 ttcgtttgtc acgggtttct tctagaagag cccgtcggga gaccaccaca tttccccgac
     2281 gatgattaat cttaataatt ttaaagttat acgttttccc gaccagacca tccaggtctc
     2341 tgactggtgt caagtcaatt tgcgatcctg gtaaaaaggc cttcacccca atatcaacga
     2401 tcatgccacc cttaatacgc gaaatgactt tcccttgcac ttgcttttca tctttgtggg
     2461 caacttccaa atcctcccaa accttcattt tgtcagcttt ttctttggag aggagcaaat
     2521 taccatccgc atcttcgcat tgctcgatat acacttgaac cgggtcattg accgccaact
     2581 gttccaactc ttccggagag aattggtcgg cagggatcat gccttcagac ttatagccaa
     2641 tatccaccac cactcgatct cgctggatat caatgaccct gccttcggtt atcgtacctt
     2701 cttcaatatt tttaaacgtt tcatcgtata atgcggcaag ggtttcgcga tccatttttt
     2761 caggttgttg agtaagagta ttcatccgac ttttgatacc tccagcagag tttggcgggt
     2821 taaatggtta ttgccatgat tagtctcaca atttacttct taaatctgtc tcatacgcct
     2881 gccgggtcgg agagggaacg ccacccaatt gagcaatatg atccatcaca gttaccgata
     2941 cgtcttcata aaatgatttg gcactctccc catcatcttt tttacaaaat ttcaatggtt
     3001 tcccaaagct gacccttaac ggaaacaacc gtggccatct tgcccggatg ggaagaactg
     3061 taaaggtacc agaaatataa acaggaatca cctggcaatt tgattcagcc actaaatagc
     3121 ctagtccagg ctttccgatt tgcaaggccc cgtcctcggt ccttgttccc tcagggaaaa
     3181 taactacggg ttcgcctgcc tgcaagtgag agagtgcctg tccaaaggcc ttcctgtcca
     3241 atttgtgggt tttcagtggg atccagccaa gcttttgcaa ggcccaattc atgtagcgat
     3301 tggggaataa gctcgctctc cctaaaaaaa aaacgcgccg cggaatcgcg caccctaaca
     3361 ggggaatatc aaggtagctg gcatggttag ccgcaacgat gattccgcct gtcctgggaa
     3421 agtgttgtaa accctctgtc ctaaatcgaa acaggagtcg cgccaaccca ttaaacagga
     3481 tccataacag cccgtaagcc gctccactca caaacgatcc acaatgattt gcatcatacg
     3541 tccaacaact tcttcaacag attgtcggga ggtgttgatc ataatcgcct caggcgccgg
     3601 ccgtaacgga tcaaccgttc tggtccggtc acgatcatct cggacggcca tggcgtctcg
     3661 caccgtctcc agatcaacgg ctttcccttg ttgacccaat tcttgttggc gccggtttgc
     3721 tcgaacctcc aattccgcat caagaaaaaa cttcacatca gcacttggga acacccgtgt
     3781 ccccatatcc cttccttcag cgataacgcc aacttcacca ccaatttttc gctgaatcgg
     3841 gagtaaccag tccctgaccg atcgaattgc cgcaacgcag gaagccaatt ggctaatggc
     3901 tggagttctg atggcctcct gattaatggc ctgcccatca acccaaacgg aaaggccatc
     3961 cccactcagc atcagtcgca gatctgtccg tgcaagaagt ccttggatgt gagcagtatt
     4021 gttcgggtct atttggtctt cctgaacttt ccaggcaatg gcccgatata aagcacccgt
     4081 atccaggtaa acatatccga gacgtgtagc caaacacttg gccacagtgc ttttcccgac
     4141 tcccgctgga ccgtcaatag tgatcattaa ccgatgacgt tgaacggccc tattctcctc
     4201 ttaccaacta atttctggag cattataatc gtcgctaaga atttgtcaat aaatccaaca
     4261 gcttatcttt aaagcccggg aaggaggttt caacgcaagc cacatcctca atggtaaccg
     4321 gagactctgc cactaatccg caaatggcca atgacatcac cacccgatga tcgccata
//
LOCUS       DAORAQ010000210         4328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2886_length_4328_cov_70.640300, whole genome shotgun sequence.
ACCESSION   DAORAQ010000210 DAORAQ010000000
VERSION     DAORAQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_4328_cov_70.640300"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKM72_12800"
     CDS             complement(<1..798)
                     /locus_tag="PKM72_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61715.1"
                     /translation="MATTAPIPSSSPILVVDDEQDIVLTLRDLLEAEGHQINVASTGV
                     TALECVKHHSPSVVILDVKLPDMDGLLVLEKLTKAVPGLPIIILSSYTTLDDVTGPLE
                     EQGAFAYLHKPINRSEIRSTVRQALKAYALAKKMERARHALYESEIRFQAVFQAAIDA
                     IVLADHTGRIMSWNKAAESMFEYTAEEMFGKPLTLIMPSRYREAHTKGIQRLQTTGEA
                     HVIGKTVVLHGLRKSGQEFPIELSLNSWTTGTHPSYSGFIRDLSLREP"
     gene            complement(1147..1335)
                     /locus_tag="PKM72_12805"
     CDS             complement(1147..1335)
                     /locus_tag="PKM72_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61716.1"
                     /translation="MHRFPRSITSISSHILPTGLSKYFYVSAINDKITDSDFEADEMS
                     AHHNFAFDKVRLLDLEGK"
     gene            1611..>1840
                     /locus_tag="PKM72_12810"
     CDS             1611..>1840
                     /locus_tag="PKM72_12810"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNP61717.1"
                     /translation="MVIPIQERLIVALDVSSVEEARRYVKDLEGVVWFYKIGLELFLA
                     VNVDFIKELRAQNCRIFLDLKMNDIDETVRRAV"
     assembly_gap    1841..1940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1941..2156
                     /locus_tag="PKM72_12815"
     CDS             <1941..2156
                     /locus_tag="PKM72_12815"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNP61718.1"
                     /translation="PGVRPAGQNTHEHQRSLTPFDAIRAGADYLVVGRPIRDADNRRK
                     MAQAIQEEIVLAVKAGPSKKQALVGGR"
     gene            2962..>4328
                     /locus_tag="PKM72_12820"
     CDS             2962..>4328
                     /locus_tag="PKM72_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNP61719.1"
                     /translation="MTMRREWLRILIVEDRPSQLEMNVNDLAEEGYQVVGARTAAEAS
                     EFARCESFAVAVVNVQAPDAAGVSLLSLLKSKNAEIQMVLSAAFASLDSAKGVIGESA
                     VSYIKREGSPSELLGIIRRTFRDQLTEYTKGLERALTERDERFRQLVDHIKEVFWVFA
                     PDQSEVWYVSPQYETVWGRSCASFREDPQSLWNAIHPQDQPRFLTHQIEPDGNGVQGE
                     CRIVRPDNTIRWVRIQTVPIRKSNGDLLSLAGVAEDITERKRVEEALTKTERQFRQSS
                     RMEAIGTLAGGIAHDFNNILTAILGYTELALATVPKESRTQRNLQEVLTAGHRAKHLV
                     LQILTFSRQAGQGKKPTPLHMVVREALKLLRSTIPTTIEIRQSLKTESTILADPTQMH
                     QIIINLCTNAEYAMREPGGILYVALEDVEVTKDLTGAISGLHIGPHVRLTVEDTGSGM
                     TPEVL"
BASE COUNT         1102 a          857 c         1118 g         1151 t
ORIGIN      
        1 tggttctcgc aaggagaggt ctcgaataaa tccggaatag gaaggatgcg ttcctgtcgt
       61 ccaggagttt aaagaaagtt caatggggaa ttcctgaccg cttttcctta atccatggag
      121 tacgaccgtt tttccgatga cgtgagcttc gcctgtcgtc tgcaatcgtt gtatgccttt
      181 ggtatgagct tcccgatacc gactgggcat gatcagtgtc agtggttttc caaacatttc
      241 ctctgcggta tattcgaaca tcgattcagc agccttattc cacgacatga tgcggccggt
      301 gtgatcggct aatacaatgg catcaatggc tgcttgaaag acggcttgaa agcggatttc
      361 gctttcatac aaggcgtgac gggcgcgttc catttttttt gccagggcat aagccttgag
      421 tgcctgccgg accgtggacc taatttcgga acgattgatg ggtttatgca aataggcgaa
      481 agcgccttgt tcttcaaggg ggccggtcac atcatcaaga gtggtataac tcgacaaaat
      541 aatgatggga agacctggca ccgcttttgt taacttttct agaacgagta agccatccat
      601 gtcagggagt ttgacgtcca ggatcacgac actaggcgag tgatgtttca cgcattccaa
      661 ggcggtcact cctgttgagg ccacattaat ttgatgtcct tcggcctcga gaagatctcg
      721 taaggttagg acaatatcct gttcatcgtc gacaacgaga attggggaag aggacggtat
      781 tggagcggtg gttgccatgg tttaaacctc atgcctgggg aaactgacgt gttcaatatg
      841 gatcttcatg agaatggccg tatgatgaat acccacgcaa tctatgtacc attaacggga
      901 agggtgaatc ggttaggcga taagtctttg aatgaagcgg taatttttga aaattattgc
      961 aaggaaaagt ccatttatcg tagattttca tttatgagag acggaaattt gatatctgtt
     1021 tggatacatc catatatcca aatggataca caaccgtgat ctgtatactg cccgggatga
     1081 aattgacgga gaagggtact gcagaatttg acctggcttt cccatccgtt tcttttacta
     1141 gatcgactat ttaccttcca gatccaacaa tcgaacttta tcaaacgcaa aattgtgatg
     1201 ggcactcatc tcatcggctt cgaaatccga gtcggtgatt ttgtcattta tggcagaaac
     1261 ataaaaatat ttggacaatc cggtgggtag gatgtgtgag gaaatagagg taatagatcg
     1321 aggaaatcta tgcatccatg gtgcttaagt tcggatcgat gagccaaaaa tggcaggttc
     1381 ggtcccgatg tgttgtcgct tgagttcatc gattggatat ggcactctcc gctggtgatt
     1441 aatatgattt ggcccatatt catggggcaa tcgggtctgt tgctaattct taattgagaa
     1501 agcctgatat atgtccacgg atctctctcc ttggactgta cacagattcc tcatcctctc
     1561 attcccataa atgggtagtc actaaagtat atagtgagtt tggagaaaat atggttatcc
     1621 caatacagga gcggttaatt gtcgcacttg atgtgtcctc tgtagaggaa gctcgtcgat
     1681 atgtgaaaga tttagagggt gtggtctggt tttataaaat tggccttgaa ttatttctgg
     1741 ccgttaatgt ggattttatt aaggaattac gcgcacaaaa ttgccggatc tttttagatc
     1801 tgaaaatgaa tgatatcgat gaaaccgtgc gtcgagccgt nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn ccaggtgtga gaccggccgg gcagaacacc catgaacatc
     1981 aacgttcgtt aactcctttt gatgccattc gagccggggc ggattattta gtcgttgggc
     2041 gacctattcg cgatgcggat aatcggcgga aaatggcgca agcgattcaa gaggagattg
     2101 tcctggcggt caaggctggt ccctcgaaaa agcaggcatt ggttggggga agatagctgg
     2161 ggtgccacag cagcaggcta aagctatcaa agtggccttc atgaaattac caaattgccg
     2221 gcacgttttt tttcttccct tccttccttt agggatgtct aacctttttc catgtttcct
     2281 tttgtctcta ggctattcgc catacttccc ttaagggtta gcatgatgaa acgagtcctt
     2341 ttctattgag taatcatgag tgaactgtga tgtcttgtcg taacggattg tgatccaccg
     2401 tttttttgaa catcacagag gagcaattgg aattgccaat gaatctctcc ctgatattca
     2461 ttcctttcct taaccttctc ccttttctta agggggagta tagtgcgtga ccattaatgc
     2521 ggggattggt atgatggaat cggatgagtg gaaaaaacag ggaattgatc ttcagagggc
     2581 tgggagccat gatcaattcc atgagggaaa cagtatcata tgtcgtggat gctgaaattc
     2641 ggtgtgtcgg agtagcctga gtattcctga tatttcagaa aaagagtgat gtggaaatag
     2701 aactgtgaga cttggggtgt atcgttgaag ttttttccaa agtgggttca aaaacaagta
     2761 tatgccgcta atcacctggg caacgggact atctcccaca tgatgagaag agtaaatgtc
     2821 tgataggtcg atgaaatgta gaccttggtt ttggatggct ggatccaaat gattcaaagg
     2881 gaagaagctc caaatagacc ttcgaataga ttttaaggat gacgaacagg gttctggacg
     2941 gtaggcatta gggagggatg tatgacgatg cgaagggaat ggttgcgaat tctcattgtg
     3001 gaagaccgtc caagccaatt ggaaatgaat gtcaatgatc tggctgagga aggctatcag
     3061 gtggtggggg ccaggacggc tgcggaagct tcggagtttg ccagatgtga aagttttgcg
     3121 gtcgcagtgg tcaatgtgca agcgccagat gccgccggag tttcattact ttctcttctc
     3181 aagtcgaaaa atgcagaaat tcagatggtc ctgagtgcag cctttgccag cctggattca
     3241 gcgaagggtg tcataggtga gagcgccgtt tcctacataa aacgggaagg gtctccatcc
     3301 gaattacttg gtattattcg tcgaacgttt cgtgaccaac tgacggaata caccaaaggc
     3361 ctggagagag ctctgacaga gcgtgatgaa cggtttcggc aactggtgga tcatattaag
     3421 gaagtgtttt gggtctttgc ccccgaccag tccgaagtct ggtatgtgag ccctcaatat
     3481 gaaaccgtct ggggtcgcag ttgtgcaagt tttagagagg accctcaaag cctatggaat
     3541 gctattcatc ctcaagatca accacgattt ctcactcacc aaattgagcc ggatgggaat
     3601 ggggttcagg gagaatgccg gattgtgaga ccggataata ctattcggtg ggtgcgaata
     3661 caaacagttc caattcgaaa gtccaatggg gacttgctga gtcttgcggg agttgcggag
     3721 gacattacag aacggaagcg tgtggaggag gctctcacga aaacggagcg ccaatttcgg
     3781 caatcaagcc gcatggaagc tattgggacg ttggcaggtg gcatcgccca tgattttaat
     3841 aatattttaa cggcaatatt gggctacacc gaattggccc tggccacggt tccaaaggaa
     3901 agccgtactc aacgaaatct gcaagaagtg ctgactgcgg gtcaccgagc caagcactta
     3961 gtgcttcaaa ttttgacctt tagtcgtcaa gctggtcagg ggaagaagcc cacacctctt
     4021 cacatggtcg tccgggaagc cttgaaactc ttacgatcta cgattcccac caccattgag
     4081 atacgtcagt cattgaaaac agagtccacc attctcgcgg atccgaccca aatgcatcag
     4141 atcattatca atctatgcac caacgctgaa tatgctatgc gagaacccgg ggggattttg
     4201 tacgttgctc tggaggatgt ggaggtgaca aaggacctga ccggagctat ctcgggatta
     4261 catattggtc cgcatgttcg gttgacggtg gaagataccg gttcgggcat gactcctgag
     4321 gtacttga
//
LOCUS       DAORAQ010000211         4305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2901_length_4305_cov_63.477412, whole genome shotgun sequence.
ACCESSION   DAORAQ010000211 DAORAQ010000000
VERSION     DAORAQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_4305_cov_63.477412"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1614)
                     /locus_tag="PKM72_12825"
     CDS             complement(244..1614)
                     /locus_tag="PKM72_12825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP61720.1"
                     /translation="MGTFIDLVDVAKQAIRHHGGVSMVFRRLWQVIAFEGFCGLQKKI
                     RQLLANRKAFSSSHESDGTVRIDAAINSEEIFSSRRAVLTSGALLLGHPFGVLGMGEH
                     VRLSASAFSAAEVPFSIRNIYGEYGLHLAEIHQDFPFRDKIAQNGSYRANVFHINADE
                     MVNAKIHLGKDIFADRYNIGYWAWELSRFPHAWCSALQLVDEVWAPSRFIEQAIADAT
                     SSPVIRMPLAVEFPAPNGMTRESFGLPDDRFLFLFFFDFTSYVQRKNPEGAIRAFLQA
                     FPDVDDERVGIVIKMNGMDLRPQEYQAFIQSIDCEDPRIILMDRVLTDRETKSLVNLC
                     DCFLSLHRSEGFGRGLAEAMYLGKPVIATGYSGNLDFTNASNSCLVDYRLIPVREHEY
                     PFAEGQKWADPDIEHAVWFMKRIVKDTHYANTIGMHAADFIKTNHSLRAVGAKYRGRL
                     AALNLI"
     gene            2736..3725
                     /locus_tag="PKM72_12830"
     CDS             2736..3725
                     /locus_tag="PKM72_12830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP61721.1"
                     /translation="MLLYVDTIDACNLKCPTCVRGSRALPNTGEKMSLAMFRQIVEKA
                     KDDGDVHQVDLFNWIEPFLNKNLHEYTNVVKDAGLPCGISSALSLPRIHNLEETLVNT
                     NSLTVSVSGIDQEVYEVNHVGGNIDYVKHHLETISRLLRTGAIRTLVSLRFLKFDYNA
                     DQETKLSDYASNLGLNFEVLPASGHPIKWALDDQAGEAVRDRLAHFSAERPHESHGHV
                     CPLIFEHIAINHAGDVYLCCAYGNFDFLRIGPYLELSKEEILLRRYTHPMCNSCAWER
                     RPATFKEQETLKKSFANRFKETPDLARKSVLDKLIQIIPAPTKGILQERIKQS"
     gene            complement(4028..>4305)
                     /locus_tag="PKM72_12835"
     CDS             complement(4028..>4305)
                     /locus_tag="PKM72_12835"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNP61722.1"
                     /translation="TWPSYKLFKGVMPSWDNTARRQDVSHVFVGATPERYEYWLRRVV
                     EQTRRLHFGDERIVFINAWNEWAEGNHLEPDREFGHQYLVATKNALG"
BASE COUNT         1265 a         1093 c          859 g         1088 t
ORIGIN      
        1 cattgcaatc cccatcctct ggcatagcca tcgcgaacat tccgggactt ccggatgggg
       61 tagtctcctc cgaaaggggg aactaataaa ctaaagacca tttccacttc actcaggtca
      121 ggcttcattt cactggcatc cctgtgttac acacaatcaa agtccttata cagcaccgct
      181 tgagaaccgg gcctatcaag atccatttat ccaaaaaggg gatcgtattt cacttcacaa
      241 ctcttaaatt aagtttaaag ctgcaagcct tcctcgatac tttgcaccca ccgcccgtaa
      301 gctatggttg gttttaatga aatctgccgc atgcatgcca attgtattgg cataatgcgt
      361 atccttgacg attcgcttca taaaccacac agcatgctca atgtcaggat cagcccattt
      421 ctgaccctct gcaaaaggat attcatgttc cctgaccgga ataagccgat aatcgacgag
      481 acacgaattg gacgcgttcg taaaatcaag attccccgaa tatccggtcg caattaccgg
      541 ttttcccaga tacatggctt ccgccaaccc ccgcccaaaa ccttctgacc gatgcagtga
      601 caaaaaacaa tcgcacaggt ttaccagaga cttcgtttcc cggtcagtca acaccctatc
      661 catcaaaata attcgaggat cctcacaatc aattgattga ataaatgcct ggtattcctg
      721 aggacgtaag tccatgccat tcatttttat cacaataccc acccgttcat catccacatc
      781 cggaaatgcc tgcaaaaatg cccgaatggc tccctctgga tttttacgtt ggacatacga
      841 ggtaaaatcg aaaaagaata aaaacaaaaa ccgatcatcg ggcaatccga aagattccct
      901 ggtcatcccg tttggcgccg gaaactcaac ggccagtggc attctgatga cgggtgaaga
      961 ggtcgcatcg gcaatcgcct gttcaataaa ccgtgacgga gcccacacct catcgacaag
     1021 ctgaagagcc gaacaccacg catgagggaa ccggctcagc tcccatgccc aatatccaat
     1081 attatatcga tccgcaaaaa tatcctttcc taggtgtatt ttcgcattca ccatttcgtc
     1141 tgcatttatg tgaaagacat tggctctgta ggaaccattt tgggcgatct tgtctcgaaa
     1201 cgggaaatcc tgatggatct cggcaagatg caatccatat tctccataaa tattacgaat
     1261 actgaatggg acctctgcag ccgaaaacgc tgaagccgac aatcgaacat gctcacccat
     1321 cccaagcacc ccaaaaggat gtccaagtaa tagggctcca gaagtaagga cagccctcct
     1381 ggagctaaaa atttcttcag aattgatcgc tgcgtctatt cgcaccgttc catctgattc
     1441 gtgactggac gagaacgctt ttcgattagc cagcaactgc ctaatttttt tttggagccc
     1501 acagaatccc tcaaaggcga tgacctgcca cagacgccga aacaccatgg aaacccctcc
     1561 atgatgacgg atagcttgct ttgccacatc cactaaatca ataaaagttc ccattgatta
     1621 tgctagccca atttcaattg ataataactc actgttgttt gtccaaaaca gaatatgaag
     1681 aggggctccc ccaatatcca taggctcaca cactttcttt tagacagcaa aggggtccaa
     1741 ctttgaaaag caggaatcct ccggccagac cttaccaaaa tatcgcccta gttgtgctaa
     1801 ttttggctgt cttgatccac agttatttga gtccaataat aaataatata tgactggcag
     1861 agggagtcat gatgaatgag cgagttttac ctagtgacgt agagactttt tcacttgctt
     1921 gggagaacca tcgtgaaacc accattcacc ttcttttcag aacaggactg ttgtgtgaga
     1981 atacctttga attgaggcaa atgacgtttt accgaattcc caaagttctt gatagaatgt
     2041 gggagggggg tgtccacaaa gagtcagcgg tggttcattc gagaatgctt caactcggat
     2101 ttatctaatg ccttgttgga tcaataaggg gcattatggg caattctaag cgcaacttct
     2161 cccggatgat gggcaaatga ttctgagagt gtggcagcaa aaatgacatt gggtgacacc
     2221 ttacctcccc gttgcaaaat accaaaagcg ggcaccctgc ctgcggttcc cttcgcgtct
     2281 accaccaata ggaatcatcc agctcaatgg ctcctacaac cgtccaaacc ctttcgatct
     2341 gccaagccat gcttattcgt caacgatggt aacagtaaat acctatttaa cgatgaatgc
     2401 cgaccaaatc ttcggccgtc cggtaagtca ctccccctaa acaagggttt cagcagccga
     2461 ttcgactgct aatggccgag ttgagaactt aaattgcaca aacccagcat agaaaattca
     2521 aatttcctac gccagcccaa aaattatttc ctgcaagcat ttgagacaag gatatacatt
     2581 taaccaagtg gtccacatca gttttattat atgccttgac caacgtgccc ccttccaacg
     2641 ccctgaaaat ttaaaaaaat catatcttaa actgattcac ccatgctaac ctctcagttg
     2701 catcgccttc cacaaatcca aatatgaggt gcaccatgtt gctttatgtt gacactatag
     2761 atgcatgtaa tttgaagtgt cccacatgcg ttagaggttc aagagcactc ccaaacacag
     2821 gcgagaaaat gtccttagca atgttccgac aaatcgttga aaaggccaag gatgatgggg
     2881 acgttcatca ggtggattta ttcaattgga tagagccatt tctcaacaaa aatctacatg
     2941 aatacaccaa tgtcgtcaag gacgctggac tgccttgtgg gatctccagc gcactgtccc
     3001 tcccaaggat tcataattta gaagaaaccc tcgtcaacac caatagccta accgtctcag
     3061 tctcaggcat tgatcaggag gtttacgagg ttaatcacgt cggggggaac atcgactatg
     3121 tcaaacacca tcttgaaact atctcccgtt tgctcaggac gggagcaatc aggactttgg
     3181 taagcctaag gtttcttaaa ttcgattaca acgctgacca ggaaacgaaa ctttcagatt
     3241 acgcatccaa tctcggccta aacttcgaag tacttcccgc atcaggccat ccaatcaagt
     3301 gggcattaga tgatcaagcg ggtgaggcag tccgtgaccg cctggcacat ttctcagctg
     3361 aacgtcccca tgaaagccat ggacacgtct gccccctgat tttcgagcat atcgcgatta
     3421 atcacgccgg tgacgtctat ctgtgttgtg cctatggaaa tttcgatttt ctcaggatcg
     3481 gcccctacct agaattgtcg aaggaggaga tccttctgag gcgttatacc catccaatgt
     3541 gcaattcatg cgcctgggaa cgaagaccag caactttcaa agaacaggaa acattgaaaa
     3601 agtcttttgc gaatagattt aaggaaactc cagatctggc aaggaaatcc gttctagata
     3661 aattaataca aatcattcct gccccaacca agggaattct tcaagaacga ataaagcaat
     3721 cataagctcc tttgttcgca tatccgaggg aaagaacaag gcctataacg ccttgttcat
     3781 gacgataagg tcttaaaaaa aatactcaca aacaaagcct tcctaaacac aaagatggtc
     3841 agtcaaacat tctgcataac tttcttcgaa ggcttttagc cataaagctt ccacgcatct
     3901 cagattgaga aagcaaccca ctcaacctcg agttggctat atctgttcca tgtaatgggg
     3961 attttgcgtt ttccaagaaa ccaattaaac atgtacagtt tttcggataa aaaatgtgaa
     4021 tagtagttta tccgagagca tttttggtgg ccaccagata ttgatgacca aattcccgat
     4081 caggctctaa gtgattacct tcggcccatt cgttccacgc attaataaag actatccgtt
     4141 catctccaaa gtgaagccga cgtgtttgtt caacgacccg tcgcaaccag tattcatatc
     4201 gttcgggagt tgcacccaca aagacatgag agacatcttg ccgacgcgcg gtattgtccc
     4261 acgaaggcat cacgccttta aagagtttgt aggatggcca agttc
//
LOCUS       DAORAQ010000212         4291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2911_length_4291_cov_63.653211, whole genome shotgun sequence.
ACCESSION   DAORAQ010000212 DAORAQ010000000
VERSION     DAORAQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2911_length_4291_cov_63.653211"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="PKM72_12840"
     CDS             complement(<1..2399)
                     /locus_tag="PKM72_12840"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNP61723.1"
                     /translation="MKPLLALEPRILFDGAALVTGAEVIQDQTSQDQSIPGPDAPAEP
                     ISGEIMDPDSENSWASGLSSATPTDRREIVFIDTRVEDYQTLMKGIHPSAEVILLDAG
                     RDGLEQIAEVLKDRNAVDAIHLIGEGTEAELHLGTAFLTNDSISNQYAAVLSQIGQSL
                     SSEADLLIYGCNFGRGQAGLSAMQTLGELTGADIAASTDRTGHVSEYANWNLEASTGF
                     IETSIVIGEAAQAAWEGVLATFTVTTTTDGGAGSLRQAIMDANANSGTDTITFAGSGT
                     YLLTIAGTGEDSAATGDLDITDNLILIGNGAANTIIDASGLGVDGVFHILGSNTTTIS
                     GITIQGGNANDGGGIFVDGSSILNLTDAIVQNNSSDRGGGLHVHGTANVNRVLFFNNS
                     ATVEGGAIHFHGADGGSLTNITVTGNTSGIDGGGLWTDTSITITNSTFTLNHANDDGG
                     GIVADAGTITISNTIVSDNTATTANPDVDGTFSSNGFNLIKDVTGGTGFGSDITGVSA
                     NLGALTNNGGPTRTHALMAGSLALNAGTTTGAPAIDQRGITRDAVPDIGAFEYTASAF
                     VSKSEFMVNDPSGDNEQTSGPLRGAERAVDIAPNGDYVVVWTNGTSNDKVFAKVFDSG
                     GNEKIAQFQVNIGGGTNAWTDVAVDDSGNFLVTWTQGGDIFMRRFLANGTAIDAGDVI
                     VNTGTSNTQQNPSVNINGAGDFVIAWEGDGGGNEGIFVRRGSMGGGLTGSDITVNTAA
                     AAKDPSVGIADSGGFVVVWDNGSDVFFQLYNSGGVSQNSGQVDVLLQLSAGAAAVDMS
                     G"
     gene            3069..>4291
                     /locus_tag="PKM72_12845"
     CDS             3069..>4291
                     /locus_tag="PKM72_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNP61724.1"
                     /translation="MREISHAGQRSHSGSYYPEASLRGHKTRAGVNCIRWIINHLAMA
                     LHIQKFLIDVVVIGLVLLDSGCAVMPTPLTQEEIQQRVQEDLAQLTQYQEPVARPITL
                     FEAMARALKYNLETRVQGLKEMVTHRQLDLAHYDMLPKVVADAAYNGRSNFAGASSQS
                     LETGQQSLVSSTSSDKDIYTSNLTLSWDVLDFGLSYVRAKQAADDVLIAEEDKRRIAN
                     RVIQDVRSAFWKAVGAERALGRLAFLQDWVTQALGEAHLIRERALADPLTSLQYEREL
                     LSAQREIQQLYQELSLSRIHLAELMNLNPGEPYELAVPEHPPLVSKVEEKLEDLEYRA
                     LMNRPELRKVDYQKRINAKETKAAILELLPNLNVYFGGNYDSNNFLFHNNWLNYGAKV
                     SWNLLNVFRHPVRLQV"
BASE COUNT         1027 a         1108 c         1079 g         1077 t
ORIGIN      
        1 tcgccactca tatccactgc ggcggcaccg gcactgagct gtaagagcac gtcaacctga
       61 ccgctattct gtgaaacccc tccgctgtta tagagctgaa agaaaacatc ggacccgtta
      121 tcccagacca cgacaaatcc accagaatcc gcaatcccta ccgagggatc cttcgcagcc
      181 gctgcggtgt tgacggtgat gtcactgccg gtcaaccctc cgcccatgct gccgcgtcgt
      241 acaaaaattc cttcgttgcc cccgccgtcg ccttcccatg cgatcacgaa atcaccggcg
      301 ccattgatgt tcacggatgg attttgctga gtgttggatg tgccggtgtt gacgatcacg
      361 tctccggcgt cgatcgctgt gccgttggcc aaaaagcgtc gcatgaaaat atccccgcct
      421 tgggtccagg taacgagaaa attcccgctg tcgtcgacgg cgacatcggt ccacgcattc
      481 gttccgcccc cgatgttgac ttgaaactgc gcaatcttct cgttgccacc actatcgaac
      541 accttggcga acactttgtc gtttgaagtt ccgttcgtcc agaccaccac gtaatcgccg
      601 ttgggtgcga tatcaaccgc ccgctcggcc cctcgcaagg gcccgctggt ttgttcattg
      661 tccccggaag gatcgttgac cataaattca ctcttcgaga caaaggcgga ggccgtatat
      721 tcgaacgccc cgatgtccgg tacggcatca cgcgtgatgc cccgctgatc aatcgcaggt
      781 gcccctgttg ttgtgccggc gttgagtgca aggctaccgg ccattagcgc atgtgttcga
      841 gtcggcccgc cgttgttggt aagtgccccg agattggcgc tgacgccggt aatgtcgctg
      901 ccaaaaccgg tccctcctgt tacatctttg atcaggttga agccattgct gctgaaggtg
      961 ccatcgacat cgggatttgc agtcgttgca gtattgtcac tcacaatcgt gttggaaatt
     1021 gtgatggtgc ctgcgtccgc aacaattcca cctccatcat cattggcatg gttaagtgtg
     1081 aatgtggagt tcgttatggt aattgatgta tctgtccaca atcccccacc gtcaatcccg
     1141 ctagtgttac cggtgactgt aatgtttgtt aacgatccac cgtcagcccc atgaaagtgt
     1201 atggcacctc cttcaacagt ggcggagtta ttaaaaaaca ggacccgatt cacattggca
     1261 gtcccgtgca cgtgtaagcc tccgccacgg tcgctagaat tgttttgcac aatcgcgtcc
     1321 gtcagattca ggatgctgga accatctacg aaaatcccac caccatcgtt cgcattacct
     1381 ccttgaatgg tgatcccgct tattgtcgta gtattggatc ctaagatgtg aaacacccca
     1441 tccacaccga gaccagaagc atcaattatg gtattcgccg cgccgttccc gataaggatg
     1501 aggttatccg tgatgtccag gtcacccgtg gctgcagaat cttcccccgt tccggcaatg
     1561 gtgagcaggt aggtcccgct tccggcgaag gtaatggtat cggtgccgga attcgcattg
     1621 gcatccatga tggcttggcg gagtgaccct gcgcccccat ccgtggtcgt cgtaaccgtg
     1681 aaagtcgcca agaccccttc ccatgcggct tgggcggctt cgccaatcac aatggacgtt
     1741 tcaataaacc cggtggaggc ttccaaattc cagttggcat attcgctgac gtgcccggtc
     1801 cggtccgtac tggctgcgat atcagcccct gtcaattcgc ccaaggtttg cattgccgat
     1861 aggccagcct gaccacggcc aaagttacag ccgtagatca ataggtcggc ctcggaagac
     1921 agactctgcc caatctgact taacaccgcc gcatactgat tgctgatcga atcattggtg
     1981 agaaaggctg tgcctaaatg cagttccgct tcggtgcctt ctccaatgag atgaatggca
     2041 tccacagcat tccggtcctt gagcacctct gcaatttgtt caagtccatc ccgccccgca
     2101 tccagaagaa tgacttctgc gctagggtgg atacctttca taagggtctg gtaatcctca
     2161 acccgcgtat caatgaagac aatttcccga cgatcggttg gcgttgccga tgagagaccg
     2221 gaggcccatg agttttcact gtcaggatcc atgatttcac cggaaatggg ttccgcgggg
     2281 gcatcggggc ccggaatcga ttgatcttgg ctggtctgat cctggatcac ttccgccccg
     2341 gtcacgagag cggccccatc aaataagatc ctcggttcga gtgctaacag tggtttcatt
     2401 ctaccgacca tctccatatc aagtgatcac tggcaaagat gtttctttct gtaaatattc
     2461 tccctggttg tgttggtgta tctgccatta cgaacctatt ttttggcata attctttcat
     2521 ttgaattttt tgggtagcga taaacccaat cgaacttact tagcacctct agatcattgt
     2581 ttttcctttt gacgcattct ctcaacataa aactcctcca tctccctatt cggctattgg
     2641 cttcattgac tggaagacat gaggggaaat tttcaccatc aaaaaaattt tggggtggtg
     2701 gatctgaatg gatgctggaa tatgggtgtc ctatctagaa gatcggatcg gattcttgat
     2761 tcctcaaaaa acagtcaaaa tagggttcat aataaaaatg acatcacgtg tggtggaaca
     2821 atgaggtgaa tttgaaaata aattttggat gccatatagc tcaaacaatg agatcacaag
     2881 acgttgtgat ggattggtaa agattgtttc acatctgggc agttctcgta taaatgagaa
     2941 cgatctacga tattaatctt gccaaaagaa cgtccgagaa acagagctgg aaagctgtta
     3001 aacggcagct ctggtttcta ttgtgaacaa aaatgctcga agggcccgta aggataaacg
     3061 gcctagagtt gagggaaatt tcacatgccg gtcagcggag tcattcgggc agctactatc
     3121 ctgaggcaag tttgcgcggg cataagacgc gtgctggagt gaattgtatc agatggatca
     3181 ttaaccattt agcgatggca ttacacattc aaaaatttct gatcgatgtg gtggtgatag
     3241 gattggtcct gctggactcc ggttgtgcgg tcatgcccac tccactcact caggaagaaa
     3301 ttcaacagcg ggtccaagag gacctggcgc aactcacgca atatcaggag cccgtggctc
     3361 gtcccattac gctctttgaa gcgatggcgc gggcgttgaa atataatctg gaaacgcggg
     3421 tgcagggatt aaaggaaatg gtcacgcatc gccaactgga tctggcgcat tacgatatgc
     3481 tgccaaaagt ggtggcggat gcggcctata atggtcgcag taattttgcg ggagcgagca
     3541 gccagtcctt agaaacaggc caacaatcac tcgtgtcctc cacttcatct gataaggata
     3601 tttacacatc gaatttaaca ttgagctggg atgtcctgga tttcggtcta tcctatgtac
     3661 gggctaaaca agccgctgac gatgtactca ttgcggaaga agataaacgt cgcattgcca
     3721 atcgggtaat ccaggatgtt cgatccgcgt tttggaaggc cgtgggagcg gagcgggcat
     3781 tgggtcgcct ggcgtttctt caggattggg taacgcaagc gctgggtgaa gcgcatttga
     3841 tccgggaacg cgccttggcg gatccgctga cctccctgca atatgaacga gagctcttga
     3901 gcgcacaacg ggaaattcaa caactctatc aggaattatc gttatcaaga attcatcttg
     3961 ccgaactcat gaatttaaac cctggtgagc cctatgaact ggctgtgccg gaacatccgc
     4021 cccttgtatc gaaagttgag gagaaactgg aagatttgga ataccgggca ctcatgaatc
     4081 gtccggaact gcgaaaagtc gactatcaaa aacgaattaa cgccaaggaa accaaagccg
     4141 ccatcctgga attgctcccg aacctgaatg tgtattttgg aggtaattat gatagcaaca
     4201 atttcctgtt tcataataat tggctgaatt acggagccaa agtcagctgg aatctgttga
     4261 atgtgttccg gcatcctgtc cgcttgcagg t
//
LOCUS       DAORAQ010000213         4287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2918_length_4287_cov_66.155009, whole genome shotgun sequence.
ACCESSION   DAORAQ010000213 DAORAQ010000000
VERSION     DAORAQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2918_length_4287_cov_66.155009"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="tenA"
                     /locus_tag="PKM72_12850"
     CDS             <1..585
                     /gene="tenA"
                     /locus_tag="PKM72_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HNP61725.1"
                     /translation="KGTLPAKKFQYYILQDARYLEELARIFALGSAKATDGDTALRFA
                     KLVEDTITVERGLHESYGKQWALSAKDMRSTPLSPTNYAYCRHMRSVAQTGTLGEITT
                     VALPCAWVYCEVGQHLLGKATPKRSHPYYEWLQLYGSPEFAEVTQWMREVVDRCAKTA
                     GRAEKARMEEAFLISSQYEWMFWDMAWREEKWPI"
     gene            693..1400
                     /locus_tag="PKM72_12855"
     CDS             693..1400
                     /locus_tag="PKM72_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61726.1"
                     /translation="MKKVKFPFLYILVLFNVCAGIQFPVQVNGQPHMAKGIQPLIQLE
                     GQSLHQLIPRDTRPLLLPEEQEAFLQELEGHPPDWPTLQSPDHTEQSKRLFQLNRARD
                     EARLIHKNILQKPIAFLWSGFLRQYMPEYEGYSVALGPELTSTSWGIVRFKPVGLPDY
                     LVAIPSLELVTLIRRQQQTGKQIAIGILFFGTLVADESLIYSFSHDQKGDGMILPVVQ
                     IEGVRYFLFPPNPSSSH"
     gene            1460..1849
                     /locus_tag="PKM72_12860"
     CDS             1460..1849
                     /locus_tag="PKM72_12860"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNP61727.1"
                     /translation="MLLKYWGPVVLYALVIVYLSSQSNPAQQLHLPSFLFKINDKILH
                     ACEYGVLGILLYRAFKYTTPMAGNMGLAIICVIAFGISDEIHQWFVPQRQADIWDLVA
                     DTFGAAFLILGWVLITEKGRKRLTIFK"
     gene            1922..2563
                     /locus_tag="PKM72_12865"
     CDS             1922..2563
                     /locus_tag="PKM72_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61728.1"
                     /translation="MRKSIKKSPDDRMAILGNQNGVTYLFVMMLVVVMSIGTMEVTQQ
                     WSVIMQRDREAELQFRGTSIAKAIERFVADWEIQKSTRPNQWPRTLEELTKKSPKRYL
                     RKAYADPITGNKFALIKIGQEIHGVRSTSTETPYNQNDFKGAKTYQAIRFEAKGNTNC
                     QINPLNPNAPTNCAPAANTLPSGEPSLGNTPSTNSPIQSPSTEAPIEETSPLQ"
     gene            2759..>4287
                     /locus_tag="PKM72_12870"
     CDS             2759..>4287
                     /locus_tag="PKM72_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNP61729.1"
                     /translation="MPRNKPFCVLAMVFMMLLMSCTLRETQLGDELEQKGDFDGAVAA
                     YRDALRKDPFNKEIEDKFQSVKIRAADQHFSRGRQMLKERKIGDALQEFQMALGFDPQ
                     NIEHHTALNDVWRLKTAHQNLLDAKHMEGLGRYDEAMTLYESSVEFDPSLSEAVEGIT
                     RVVQLQKTTQAIGGSAEPVTLRFQNTRLKQVFEILARTANIDILFDKDVRDDLVTIFT
                     KDTPFDEALNLILTTNQLFAKRVGPDRLLVIPDTKQKREQYDDLQIRTFYLSNAKAKD
                     IANLLRTILETKRVYVNEPLNTVVIRDEPAKVSLAEQIILANDRSDSEVLFEVEVLEV
                     NRTNKQNLGLQFAKEAGFGIFPPGTESPLSPAFSLPSTFSFKQLTDIGQESFMFRFPS
                     SVLLNFFKQESQAKTLASPKIRVLNNQKASINVGDKQPILLSTTNVLPGQASTGAVPT
                     TSTVTSIEFKDTGIKLTVEPTIHLNNSISLRLQIEVTRLGAREILQTSPLITQFKFGT
                     RT"
BASE COUNT         1194 a         1127 c          926 g         1040 t
ORIGIN      
        1 aaaggcacgt taccggcaaa aaagtttcaa tattacatcc tacaagatgc aaggtattta
       61 gaagaattgg ccagaatctt tgctctagga tcggccaaag cgacggatgg ggacacggcc
      121 cttcgctttg caaaattagt tgaagatacc attaccgtcg aacgaggact tcatgaatcc
      181 tatggaaagc aatgggctct ttccgccaag gacatgcgat ccacccccct ttcccccacg
      241 aattatgcct actgtcggca tatgagaagc gtggctcaaa ccgggacact cggtgaaatc
      301 actacggtgg ccctaccctg tgcatgggtc tattgtgaag tcggacaaca tcttttaggg
      361 aaggcaacac ccaaacgctc acatccgtat tacgaatggt tacaactcta tggatcaccc
      421 gaatttgcgg aagtgactca atggatgcgc gaagtcgtcg atcgatgcgc caagacagca
      481 ggccgggcag aaaaagccag aatggaagaa gcctttttaa ttagttcaca atacgaatgg
      541 atgttttggg atatggcctg gcgggaagag aaatggccca tataggacat tacattcctt
      601 aatctttaaa caacatgacg tggacagcat tcactgcttt caaaactctg cccgttcatt
      661 cacgcaattt ctcccggtga caacgcaagc acatgaaaaa agtcaaattc ccctttcttt
      721 atattctggt gttattcaat gtatgtgcag ggattcaatt tccggtccag gtcaatggac
      781 aacctcacat ggcaaaagga atccaacccc ttatccaact ggaaggccag tccttgcacc
      841 aactgatccc cagggatacc cgtcccctcc tccttccaga agaacaggaa gcatttcttc
      901 aggaacttga aggccatcca ccggattggc ccaccttaca gagcccggat cacactgagc
      961 aaagcaagcg tctattccaa ctgaatcgcg ctcgggacga ggcccgcctc atccacaaaa
     1021 atatccttca aaaacccatt gcctttctat ggtcaggctt tctccggcaa tatatgccgg
     1081 aatacgaggg atattccgtg gccttaggtc cagaactcac ctcgacatcg tggggtattg
     1141 tgcgctttaa gcctgtgggc ttgcccgact atttggtggc catcccctct cttgaattgg
     1201 ttacgctaat tcggcgtcag caacaaacgg gtaagcaaat tgccatcggc atcctttttt
     1261 ttggtactct agttgccgat gaatctctca tctatagctt ttctcatgat cagaaggggg
     1321 acggaatgat tttgcccgtc gtccaaatcg agggtgtaag gtatttcctc ttccccccga
     1381 atccatcatc atcacattaa tccactgtta acaaaggacg tgtcagattt ctccgaccca
     1441 agccatatct aaaccattcg tgctattaaa gtattggggg ccagtcgtcc tctatgctct
     1501 tgttattgta tatctctcct cccaatccaa tcctgcccaa caactccacc tcccttcatt
     1561 tctttttaaa attaatgata aaattttaca tgcctgcgaa tatggcgtac tcgggatttt
     1621 gctctatcgg gcattcaaat acacaacccc gatggctgga aatatgggcc tcgcaatcat
     1681 ctgcgtcatc gcattcggga tttccgatga aattcatcag tggtttgtcc cccagcgtca
     1741 ggctgacatt tgggatctcg tcgccgatac ctttggcgcc gcctttctga ttcttggatg
     1801 ggtgctcatt accgagaaag gacgaaaacg gctgactatc ttcaagtaac actcacccag
     1861 caccatggca cagttgtcac caaactgata tccgcactca acaaacagac agcatcacta
     1921 catgaggaaa tctatcaaga aatccccaga tgaccggatg gccattctcg gcaaccagaa
     1981 tggagtcacc tatcttttcg tcatgatgct ggttgtcgtc atgtccattg gaacgatgga
     2041 agtcactcaa cagtggtcgg ttataatgca acgggatcga gaggccgaac tacaatttcg
     2101 tggaaccagt attgcaaaag ccattgagcg atttgtggcg gactgggaaa ttcagaaaag
     2161 cacacgtccc aaccaatggc cgcgaacgct tgaagaatta acgaagaagt caccaaaacg
     2221 gtatctccgg aaggcctacg ctgatcccat aaccggaaac aaatttgcgt tgataaaaat
     2281 tggccaggag atacatggag tcagaagtac cagcaccgaa accccatata atcagaatga
     2341 cttcaagggg gccaaaactt accaagccat ccgcttcgaa gccaagggaa acaccaactg
     2401 ccagatcaat ccgctcaatc ctaatgcgcc aaccaactgt gcccctgcgg ccaacacact
     2461 cccttctgga gaaccttccc tagggaatac cccttcgaca aattccccga ttcaatctcc
     2521 atctacagaa gccccaatcg aggaaacttc acctttacag taatcctgaa cccattcgag
     2581 cccgcaggga cgggactcaa tattgacgaa gggatatccg atacagaaat tagagtagct
     2641 taaagaaacg agcaagtaac tctagcccgt atttgacaaa tatgctaggg taaggaggat
     2701 ttgtgagatc ttcgtcttac ccccccagtc attctcatta catatagtga aggatataat
     2761 gcctagaaat aagccgtttt gcgtgttggc catggttttt atgatgcttc tgatgtcctg
     2821 cactcttcgt gaaacccagc ttggggatga acttgaacaa aaaggtgatt tcgatggagc
     2881 cgtggcggct tatcgggacg ccctgaggaa agaccccttc aataaagaaa ttgaggataa
     2941 attccaatcg gttaaaatcc gtgccgcaga tcagcatttc tcccgtggaa ggcagatgtt
     3001 aaaagagcgg aaaatcggtg acgcccttca agaatttcaa atggctctgg ggttcgatcc
     3061 acagaatatt gaacaccata ccgccctcaa cgatgtctgg cgtttaaaga ccgctcatca
     3121 aaacttgttg gatgccaaac acatggaagg tcttggccga tatgacgaag ccatgacgct
     3181 gtatgaatca tctgtggaat tcgacccctc cctgtcggag gcggttgagg gcatcacgag
     3241 agtggtccaa ttacagaaaa ccacccaagc catcggtggc tcggccgaac ccgtgaccct
     3301 tcggtttcag aatacccgtc ttaagcaagt ttttgaaatt ttggcgagaa ccgccaatat
     3361 cgatatttta tttgataagg atgtgcggga tgatctcgtg accattttca ccaaagacac
     3421 ccccttcgat gaagccctca atcttatatt aaccaccaat caactgtttg ccaaacgcgt
     3481 cgggcctgac cgactgttgg tcatccccga taccaagcaa aaacgggagc aatatgatga
     3541 cctgcaaatc cgcacgtttt atctttccaa tgccaaggcc aaagatatcg cgaatcttct
     3601 ccggacgata ctggaaacca agcgcgtcta tgtgaatgaa ccgttgaaca ccgtggtcat
     3661 acgagacgaa ccggccaagg tcagcctggc cgagcagatc atcctggcca atgatcgtag
     3721 cgactcggaa gtcctgtttg aagtggaagt cctggaagtc aaccgtacca acaaacaaaa
     3781 cctgggctta cagtttgcca aagaagccgg atttggtatt ttcccgcccg gcactgagtc
     3841 tccgttatct cccgcatttt ctcttccttc aacattttcg ttcaaacaat taacagacat
     3901 tggtcaggaa tcgtttatgt ttcggtttcc cagcagtgtc ctactgaatt ttttcaaaca
     3961 ggaatctcag gccaaaaccc tggcttcgcc taaaatcaga gtgctcaata atcagaaagc
     4021 ctccatcaat gtcggggaca aacaaccgat tcttctgtcc accacgaatg tgttaccagg
     4081 tcaggcatcc acaggtgcag ttcccaccac ttcgaccgtg acctccattg aatttaagga
     4141 tacgggcatc aaactcaccg tcgagccgac catccacttg aataacagca tttccctgcg
     4201 gttgcagata gaagtcacac gcctgggagc cagggaaata cttcaaacat ctcccttaat
     4261 tacccaattc aagtttggaa cccgtac
//
LOCUS       DAORAQ010000214         4286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2919_length_4286_cov_62.854881, whole genome shotgun sequence.
ACCESSION   DAORAQ010000214 DAORAQ010000000
VERSION     DAORAQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2919_length_4286_cov_62.854881"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKM72_12875"
     CDS             complement(<1..328)
                     /locus_tag="PKM72_12875"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNP61730.1"
                     /translation="MIGKKNLQCTSGKGWYLSGMVTLSLSALFIAGVSAAELPREAVL
                     PLSLATKAASAAVEQCTKDGYRVSAAVVDRAGVVRAILREDGAGPHTVDSSRKKAYTS
                     ASLKRPT"
     gene            complement(421..1101)
                     /locus_tag="PKM72_12880"
     CDS             complement(421..1101)
                     /locus_tag="PKM72_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNP61731.1"
                     /translation="MKGLGILVMVSFLLMFAGPGFTKEMVTKTGLNEALFEAVGRNDV
                     AMVRQLLNQGASIHAQDGKGQTALLVAVDRNHLESAKVLIEAGADVNAQDRQLDSPLL
                     LAGARGYLEILQLILEAKPDFGIYNRFGGTALIPACERGHVETVRRLLETEVDINHVN
                     QLGWTALLEAIILSDGGPRHQEIVQMLMKAGADVNLPDGDGISPLQHARTKGFTRIVN
                     ILESAGAR"
     gene            complement(1298..2602)
                     /locus_tag="PKM72_12885"
     CDS             complement(1298..2602)
                     /locus_tag="PKM72_12885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNP61732.1"
                     /translation="MITRVVMPKLTDTMEEGVVVAWKKHEGDAVDSGDILAEIETDKA
                     VLDLESFGSGVLRKVLVPEGETVKAGKLIAVIGEQDDDIEATLAETSEDKTAATEPQK
                     DYAAPSPKQKPSQPSPAPSTPTKGEKSGSAKTETQATKADTSQEVRIKASPRAKALAK
                     EQEIDLSTITGSGPDGRIVEADIQRRVKTPKVLEGEAQDIPLSQMRKAIARVTTSSKA
                     PVPHFYLTTEVAMDQAERLRKQMEELGNGPLSLTVLFVRAAALALARHPEMNVSFMGE
                     ALRRHATIDIGIAVALEEGLITPVIRDCAGKNILQVAEEIRDLFVRAKSQQLTPEEYA
                     GATFSISNLGMYEVDNFIAVLMPPQAASLAIGAVNIVPVARGRAIKLSRRMKVTLSCD
                     HRALDGAQGARFLQSFKRALEKPLELFLPLKTRSKRDHEIDQ"
     gene            complement(2694..3662)
                     /locus_tag="PKM72_12890"
     CDS             complement(2694..3662)
                     /locus_tag="PKM72_12890"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNP61733.1"
                     /translation="MLISYREALNQAMREEMQRDSRVFLIGEEVGYYQGAFKVSKGFV
                     EEFGVERVLDTPITEAGFTGLAVGAAMAGLRPIVELMTFNFGILALDQIVNNAAKVRY
                     MSGGQLSAPMVIRGPGSAAHQLAAQHSQSLEAWFCHVPGLKVIAPATPSDAKGLLKSA
                     IRDENPVIFIEAQLLYGTKGEVPDHDYSLPIGKAAVTRTGSDVTIVAYSKMALLALEA
                     AEALSKEGINAEVIDLRTLKPLDTDTLLESVKKTGRVVIVEEGWEFCGLGAQIAETIY
                     SKGFDLLDGPIVRVAGEEVPMPYTRPLEDLAIPDAARVISAVKKIL"
     gene            complement(3753..>4286)
                     /locus_tag="PKM72_12895"
     CDS             complement(3753..>4286)
                     /locus_tag="PKM72_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNP61734.1"
                     /translation="AVPSGHTHEAFNLAALWKLPVIFICENNRYGMGTPVEREMLLYK
                     DIAERARAHGIEAEQVDGMDVLAVKELTARVVKKLRKDPQPFFIEASTYRFMGHSMSD
                     PAHGHYRTKEEVGEARKQDPLIFLKTRILQDGLAKEADFKQMDKEVIQTVTESVKFAD
                     ESPFPDPGALHEDVLVE"
BASE COUNT          997 a         1263 c          965 g         1061 t
ORIGIN      
        1 ccgtaggcct tttcagactg gccgaggtat aggccttctt gcgactgcta tccacggtat
       61 gcggaccggc accatcttcc ctcaatatcg ccctcaccac cccggcccga tcgacaaccg
      121 ccgcactcac ccgatatcca tcttttgtgc attgctccac cgccgcactt gcagccttcg
      181 tggccagtga gagtggcagg accgcctcac gcggcagctc ggccgccgac acgcccgcta
      241 tgaatagtgc gcttagactg agggtaacca ttccagagag ataccaacct tttcccgatg
      301 tacattgcag attttttttg ccgatcatga aacgctcctt tgataaacat gagatcattc
      361 atgtgctgac ctgacgattg tcttgttcag gctgcaacgt tgtcaatata tctcacaccc
      421 ctaccgggct cccgccgatt ccaggatatt aacaattcgt gtaaaacctt tggtccgggc
      481 atgttgcagc gggctgatgc catccccatc cggcaggttc acatcggcac ccgccttcat
      541 aagcatttgt acaatctcct gatgtctggg ccccccgtcg ctcaggataa tggcttccaa
      601 cagggcggtc caccccaatt gattcacatg attgatatcc acctcggtct ctaataaacg
      661 cctcaccgtt tccacatgcc cacgctcaca tgccggaatc aaggccgttc ccccgaaccg
      721 gttataaatt ccaaaatcgg gtttggcctc taatatcaac tgtaaaatct cgaggtaccc
      781 tctcgctccg gctaagagca atggactgtc cagttgacga tcctgcgcat tcacatccgc
      841 gccggcctca atcaacacct tggcactttc aagatggttg cgatcaacag ccacgagtaa
      901 cgccgtttgt ccctttccgt cttgagcatg gatgctggca ccttggttca acagttgccg
      961 gaccatagcc acatcattac gtcccacagc ctcgaataag gcttcattca gccctgtctt
     1021 cgtgaccatc tctttggtaa atcccggtcc ggcaaacatc agaagaaacg acaccatcac
     1081 aaggattccc aatcctttca tatcttcgta catccccttt gtaattaatt tcggcttgga
     1141 ctcaggacag ggagggacca gataagacca tctctcaatc cggcaaagaa aatgaggaac
     1201 aggacagccg tattatctga accataaaac tccggcttat taccgcctca acacaccggc
     1261 cccaagattg aaatacccgc catcaccgca caaccggtta ttgatcgatt tcatggtccc
     1321 ttttggatcg ggttttcaac ggaagaaaca gctccaatgg tttttccagg gctcgtttaa
     1381 atgactggag aaaccgtgcc ccttgcgcac catccagtgc ccgatgatca caggaaagcg
     1441 tgactttcat ccgacgcgac agtttgatgg ctcgccctcg tgccacggga acaatattca
     1501 ccgcgcctat ggccagagaa gccgcttggg gcggcatcaa gacggcgata aaattgtcca
     1561 cctcatacat gccaaggtta gaaatggaga atgtagcccc ggcatattct tcgggagtga
     1621 gttgttggct tttggcccgg acaaacagat cacgaatctc ttcggcgact tggagaatat
     1681 tttttccggc acaatcccgt atcacagggg taatgagtcc ctcctccaga gccaccgcaa
     1741 ttccgatatc gatcgtggca tgtcgacgca aggcttcccc catgaatgaa acattcatct
     1801 cgggatgacg agctaacgcg agcgcggccg ctcgaacgaa caagaccgtt aaactcaatg
     1861 ggccattgcc caattcctcc atttgcttcc ggagacgctc ggcctgatcc attgccactt
     1921 ctgttgtgag ataaaaatgt gggacagggg ccttacttga cgtggtcact cgagcgatcg
     1981 ccttgcgcat ttggctcagg ggaatgtctt gggcttctcc ctcaaggact tttggtgttt
     2041 taacccgtct ttgaatatcg gcctctacga tccggccatc aggaccgctt cctgtgattg
     2101 ttgacaaatc aatttcctgt tctttcgcca acgccttggc tcgaggcgat gctttgatcc
     2161 tgacctcctg gctcgtatcc gccttcgttg cctgggtttc tgtcttcgca ctgccggact
     2221 tttcaccctt ggtcggggtg gatggtgccg gcgaaggttg agagggcttt tgctttggag
     2281 aaggagccgc ataatctttt tgaggttcag ttgctgcagt tttgtcctca gaggtctcag
     2341 ccagcgtggc ctctatgtca tcgtcctgtt ccccaatcac ggcaataagc ttcccggctt
     2401 tcacggtttc cccctcgggc actaacactt ttctcagcac accggaccca aaggattcca
     2461 gatcaaggac cgctttgtcc gtttcaattt ccgccagaat atcccctgag tcgacagcgt
     2521 caccctcatg ttttttccaa gccaccacga ccccttcttc catggtatcc gtgagtttgg
     2581 gcatgacaac gcgcgtaatc ataggcctct cctatttatg gcaggggaat tcttccaaag
     2641 aatctgtagt gatgacgttc tgaggaacaa ccatttcaaa aatgtgttgc cggttagaga
     2701 atttttttca ccgccgagat gacccgagct gcatctggaa tggccaggtc ttctagcggc
     2761 cgggtatacg gcatgggaac ttcctcacca gcgaccctga cgataggccc atccaataaa
     2821 tcaaatcctt ttgaatagat ggtctcagca atctgagcgc ctaacccaca aaactcccac
     2881 ccctcttcta caatgacaac acggcctgtc ttcttgacag attccaggag cgtgtcggta
     2941 tccaaaggct ttaatgttct caggtcgatg acctcggcat tgatgccttc ctttgataag
     3001 gcttcagcag cctcaagcgc cagtagtgcc atcttggaat aggcgacaat ggtcacatcg
     3061 cttcctgttc gcgtcacagc ggccttccca atcggcaaag agtaatcatg gtcgggcacc
     3121 tcgcctttgg tgccatacag aagttgcgct tcgataaaaa tgaccgggtt ctcatcacga
     3181 atggcgcttt tcaataaacc tttcgcatca gagggcgtcg ccggggcaat cactttcaga
     3241 ccagggacat ggcaaaacca ggcttcaaga ctttgcgaat gctgggcagc caactgatga
     3301 gccgcactcc ccggcccacg gatcaccatg ggagcagaca actggccgcc ggacatatag
     3361 cgaactttcg ccgcgttatt cacgatttgg tcaagggcaa gaatgccgaa attaaaggtc
     3421 atcaattcga caatgggacg caatccagcc atcgccgcgc caacagccaa acccgtaaag
     3481 cccgcttcgg taatcggggt atccaacacc cgctcaacgc cgaattcctc tacaaagcct
     3541 ttgctgacct taaaggcccc ttggtaatac ccgacttctt ctccgattaa aaaaacacga
     3601 gaatcccgtt gcatttcctc acgcatcgcc tgattcagtg cttccctata ggagatcaac
     3661 atggttggcc gtcattcaag aattgttgat aatcgagtgt cataatgaag gtaacttaat
     3721 acctttccga attcttatta agtccatgct cttcactcga ccaacacatc ctcatgaagt
     3781 gcaccgggat caggaaaggg gctttcatcg gcaaacttca cagattcggt caccgtctga
     3841 attacctctt tatccatttg cttaaagtcc gcctccttgg ccaggccatc ctgaaggatc
     3901 cgggtcttga gaaaaatcaa cggatcctgc ttccgtgctt ctcccacctc ctccttggtt
     3961 cgataatggc catgggcagg atcggacatg gaatggccca tgaaccggta ggttgacgcc
     4021 tcaataaaga atggctgagg gtcctttcgc aacttcttca ccacacgcgc cgtcaactcc
     4081 ttcaccgcca gcacgtccat cccatccact tgttccgctt caatgccatg cgctctggct
     4141 cgttcggcaa tatctttata cagcagcatt tcgcgttcaa cgggcgtacc cattccgtac
     4201 cggttgtttt cgcaaataaa gataaccggc aatttccata acgcagccag attaaaggcc
     4261 tcatgggtat gcccactggg aaccgc
//
LOCUS       DAORAQ010000215         4258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2944_length_4258_cov_59.980490, whole genome shotgun sequence.
ACCESSION   DAORAQ010000215 DAORAQ010000000
VERSION     DAORAQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2944_length_4258_cov_59.980490"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKM72_12900"
     CDS             <1..897
                     /locus_tag="PKM72_12900"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61735.1"
                     /translation="VTGSVFAAVGTALPETMVPIFAILLSSGSETLRHEVGVGAILGA
                     PLMLATLAFFLMGFFAARKRGWHGTLNPEPTGFARDLLWFNWAFCLGVIAVFVPQEWG
                     WVRSVIAGALVILYVVYLYQTIRSSANLVDDGHGTEADHPLYMTKVGLPDNLLVILFQ
                     VGCGLGLIVLGAKGFVHGIEHLAPKWGVSALTLSLLIIPIATELPEKVNSIIWIRRDR
                     DTLAVGNITGAMVFQGSLLPALGIMLTAWVPQHEILMGMAMTLVAASFLTWVVRRGGL
                     KPIHLVVNGLCYVVFVGSVLFW"
     gene            952..2010
                     /locus_tag="PKM72_12905"
     CDS             952..2010
                     /locus_tag="PKM72_12905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNP61736.1"
                     /translation="MSLPTVVVTRRLPQHALDVLRAQADLIVWEQDCPVDRKWLLKHL
                     PEAEGLYCLLTDRVDREVLQVGKRLRVISTMAVGYDHIDVAGCTARGIPVGHTPGILT
                     ETTADLAFALLLSAARRIVEGAEYVRKGQWTTWSPDLLLGQDVHGATLGIVGFGRIGE
                     AMARRAQGFRMNVLAVRSPQSSASSRSGTHHFATAILPREGSSAGNNEGESVSASFQR
                     VDLHEALAQSDFVSLHVPLTPHTHHLIGENEFRAMKPSSILINTARGAVVDPEALYHA
                     LVHGHIGGAALDVTDPEPIPMSEPLLTLPNCLVIPHLGSASVATRTRMACIAAENIVA
                     GLRGDALPHCVNPEVYRV"
     gene            2149..2460
                     /locus_tag="PKM72_12910"
     CDS             2149..2460
                     /locus_tag="PKM72_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HNP61737.1"
                     /translation="MIHITTPAVEKLKILIEEHPEDPIVRLAVRDRDDKVLVFSITLE
                     DAVTQEDSVLDIEGLIIAIDGSCATRLEGVTVDYEDVGGFSFHHPEPPDPYKLNLILP
                     E"
     gene            2573..3757
                     /locus_tag="PKM72_12915"
     CDS             2573..3757
                     /locus_tag="PKM72_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNP61738.1"
                     /translation="MEIFFATCPRGLEEVLSQELQELGGQEIRLTPGGVECRGPFPLC
                     YRLNLESRIASRILWRVGQGWYRDEEDLYRLASSLPWPEYFSVDCHIKVRIIAQHSPL
                     KSLEFATLRVKDAICDRFVRATHARPEVSKRQPDIQIVVFVDEEHVVWYIDTSGDPLF
                     KRGWRKVAGEAPIRENLAAGIIRLSGWTGEQVLLDPMCGAGTFLIEAALIARGIAPGS
                     GREFAFRHLENFDHTIWDHLRQESIKPVAPGTGLSIIGYDEDANALTMARRNLEGLGL
                     EEIQLGQVDVLDVTPPGPKGFLVTNPPYGVRMGDRSELEEWYPKFGNLLKQRFAGWDV
                     YVLTADSRLPKLIHLAPSRKIPLFNGPLESRLYEFQMVAGGNRKSARQARQQAVNNPG
                     LK"
     gene            complement(3750..>4258)
                     /locus_tag="PKM72_12920"
     CDS             complement(3750..>4258)
                     /locus_tag="PKM72_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61739.1"
                     /translation="HQTEGWAVIFNQKDGHRIMRGAVKNTIKILTIEDDTAQIEALKE
                     ALTVAQIICVEILEAEDGEQAMSFLNQEYPFMDAPQPDLIFLSNHLPDVSGEEIIKGL
                     QRDRRLQSIPVALFSEEAPRQSKDFPFPPNCHLFKRPKTCDEWIYVLRCIEDVWVSLL
                     NMAGKPLI"
BASE COUNT          925 a          962 c         1259 g         1112 t
ORIGIN      
        1 gtcaccggtt cggtgtttgc ggcagtggga acggcattgc cggaaacgat ggtgccgatt
       61 tttgccatct tgttgtcatc aggttctgaa acgttgcggc acgaagtggg ggtgggggcc
      121 attctcgggg cgcccctgat gctggccacg ttagcattct ttctgatggg gttttttgcc
      181 gcacggaaaa gagggtggca tggcacgtta aatcctgagc caacaggatt cgcccgtgat
      241 ctgctctggt ttaattgggc gttttgtttg ggagtgattg ccgtatttgt tccccaggag
      301 tggggttggg tgcggtcagt cattgcgggg gcgctggtta tcctgtatgt ggtgtatttg
      361 tatcaaacca ttcgatcttc agccaattta gtggatgatg gacatgggac ggaagcggat
      421 catccgcttt atatgaccaa agtgggcctt cccgataatt tattggtgat cttgttccaa
      481 gtgggatgcg ggctgggcct cattgtgcta ggggcgaaag gctttgtgca tggaattgaa
      541 catcttgccc caaaatgggg agtttcggcc ttaaccctgt ctctcctcat tattccaatt
      601 gcgacagaat tgccggaaaa agtgaacagc attatttgga tccggcggga tcgggatact
      661 ttggctgtcg ggaatatcac cggggccatg gtctttcagg ggagtttgtt gccggccttg
      721 ggaattatgc tgacagcctg ggtgccgcaa catgaaattc tgatgggaat ggcgatgacg
      781 ttggtggcgg cctccttttt aacctgggtc gtgcggcgtg gtgggttgaa gcccattcac
      841 ctggttgtta acggactgtg ttatgtggtg tttgttggct ctgtgctctt ctggtaggtc
      901 ctgttctttt ggtcattcaa gagccatgaa tcgtaaaaaa agagagtaac gatgtccctt
      961 ccgacagttg ttgtcacgcg acgtctccca caacatgcct tggatgtgtt acgggcgcaa
     1021 gcggatctta ttgtctggga gcaggattgc cccgttgatc ggaaatggtt attgaagcat
     1081 cttcctgagg cggagggcct gtattgcttg ttgacggacc gggttgatcg ggaggtgctt
     1141 caggttggga aacggcttcg ggtgataagc actatggctg tcggatatga tcatattgac
     1201 gtggcaggat gtacagcccg tggtatcccg gttgggcaca cgcctggtat tttaaccgaa
     1261 accactgcgg acctggcctt cgcgctgctc ctatctgcag ccaggcggat tgtagaagga
     1321 gccgagtatg tacgaaaggg acaatggaca acctggagcc cggatctctt gctgggacag
     1381 gatgtgcatg gtgcaacgtt aggcattgtg gggtttggca ggatcgggga ggcgatggcc
     1441 aggcgggctc aaggatttcg gatgaacgtg ctcgcggtaa ggtcacccca atcttctgcc
     1501 tcatccaggt ccggcacgca tcactttgcc acagcgattt tgccacggga aggctcttct
     1561 gcagggaata atgaggggga gtcggtgagt gcgtcattcc agagggttga tctgcatgag
     1621 gcattagctc agtcggattt tgtgagtctg catgtccccc tgactcctca cacgcatcat
     1681 ttgattgggg agaatgagtt tcgcgccatg aagccatcaa gtattttgat caatacggct
     1741 cgtggggcgg tggttgatcc tgaggccctc taccacgctc tagtgcatgg ccatattggc
     1801 ggtgcggcct tagatgtgac tgatccggag cccattccta tgtcagaacc gcttctcacg
     1861 cttcctaact gtctggttat cccgcacttg gggagtgctt ctgtggcgac aaggaccaga
     1921 atggcttgca ttgccgctga gaatatcgtt gccgggttac gtggagatgc gcttccacat
     1981 tgtgtcaacc ctgaagtcta ccgcgtgtaa cgccattcca aaacctggga ttgtaagaat
     2041 acacaaggaa attgatggag gattgtttat gctgggaagg gagttcgatg aaaggcgaga
     2101 cctggcaggt caagctgatg attggccgct tcgtcaagag gagaaatcat gatccacatc
     2161 acaacaccgg ctgtggaaaa attgaaaatc ttgatcgagg agcatcctga ggatcccatt
     2221 gtccgtttag ccgttcggga ccgggatgac aaagtcctcg ttttttccat tacacttgaa
     2281 gatgccgtaa cacaggagga ttcggtctta gatattgaag ggctcatcat cgcaatagac
     2341 ggctcctgtg caacacggct ggaaggtgtc actgttgatt atgaggacgt tggtggattc
     2401 agcttccatc acccggaacc gcccgatcct tataaattaa acctgatcct acctgaatga
     2461 ggccgataga atgggattgg ggcaccaatt gaacccgaat gcgggaaggt tcttcatcaa
     2521 cgattgttgt taacgtcaac cagtaacttc cacccccgcg tttattcgta ttatggaaat
     2581 attttttgcg acatgtcctc gtggcttaga agaagttctc agccaggaac tccaagaatt
     2641 gggcggacag gaaattcgat tgactcctgg cggggtcgaa tgtcggggcc cttttcctct
     2701 ctgctaccgg ctcaacttag aaagtcgcat tgccagccgt attttgtggc gagtcgggca
     2761 ggggtggtac cgggatgagg aggatctcta tcgccttgcc tcaagtcttc cctggcccga
     2821 atatttttcc gtggactgtc acatcaaagt tcgcatcatt gctcagcatt ctccactgaa
     2881 aagcctggag tttgcgacgt tgcgagtgaa agatgccatt tgtgaccgat ttgttcgggc
     2941 gacacatgca cgccccgagg tgagcaaacg ccaacccgat attcaaattg tggtctttgt
     3001 cgatgaagag catgttgtgt ggtatatcga cacctcggga gatccgctgt ttaaacgtgg
     3061 atggcggaag gtggccggag aagcgccgat acgagaaaat ttggccgccg gcatcatccg
     3121 gctttcaggt tggaccggcg agcaggtctt acttgatccg atgtgtggcg cgggcacatt
     3181 tttaatcgag gcggcgttga tagccagagg gattgcccca ggtagtgggc gggaatttgc
     3241 ctttcggcac ctggagaatt ttgatcacac gatctgggat cacctacggc aagagtcgat
     3301 caaaccggtc gctcccggta cgggtctctc aattattggg tatgatgagg atgcgaatgc
     3361 cttgaccatg gcaagaagaa atctggaagg cttggggctt gaggaaattc aattgggtca
     3421 ggttgatgtg ttggatgtaa cgcctccagg gccaaagggt tttctggtca ccaatcctcc
     3481 ctatggagtg agaatgggag atcggtcgga gctggaggag tggtatccga agtttgggaa
     3541 tctcttgaag cagcggtttg ctgggtggga tgtctatgtg ctcacggccg attcgcgtct
     3601 tcctaaactc attcatttag cgccgtcacg caaaatcccc ttgttcaacg ggccgttgga
     3661 atcaagattg tatgaatttc agatggtggc cggaggtaac cggaaatcgg ccaggcaggc
     3721 caggcaacag gcggtgaata accccggact taaatgagag gtttcccggc catgtttaaa
     3781 agcgagaccc agacatcttc aatgcatcgc agcacataga tccattcgtc gcaggtcttc
     3841 ggtcgtttga atagatggca attgggaggg aaaggaaaat ctttcgattg acggggagcc
     3901 tcctccgaaa acaacgccac cggaatggac tgtaagcggc ggtcgcgttg gagtccctta
     3961 ataatctctt ctcccgacac atcggggaga tgattggaga gaaaaatcag gtcgggttgt
     4021 ggggcatcca taaagggata ttcctgattt aaaaaggaca tggcttgttc gccgtcttcg
     4081 gcctcaagaa tttccacaca gatgatttgg gctaccgtca gggcttcctt gagggcttct
     4141 atctgggcgg tatcatcctc aatcgtcagg atctttattg tgtttttgac ggctccgcgc
     4201 atgatccgat gcccatcttt ctggttgaaa atgactgccc acccttcagt ctggtgaa
//
LOCUS       DAORAQ010000216         4250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2950_length_4250_cov_68.967580, whole genome shotgun sequence.
ACCESSION   DAORAQ010000216 DAORAQ010000000
VERSION     DAORAQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2950_length_4250_cov_68.967580"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            520..1506
                     /locus_tag="PKM72_12925"
     CDS             520..1506
                     /locus_tag="PKM72_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNP61740.1"
                     /translation="MLKATWVAEKPFSNQIRQPAKHHSYKRFRSSQALAGSFILHALF
                     LSICYSVAFQASEPKREIELSTFRWNVSLTTAPIPEPVTSDTPSPLLSAPPQETVVAH
                     SESSHHLTRNASSKHSRSTLEGGRKKLSTDLSVQSKTIAFPLTEFPVKQSLNTEKQAR
                     PPSKTTTAHSQPSTQEQINTVPFTHGQTAKLPPPVKRVVDRAERVMQPSAQREPKIIH
                     RTRVFHPPIQRRETRPDYGWLITDLRKTLERLKFYPEIARLNKWEGKVLVQMKVLEGG
                     HLIDMEIQESSGFEVLDQTALTIVRQASPLDLGHPLPSNSVSLSVPLTFQLD"
     gene            1985..2410
                     /gene="exbB"
                     /locus_tag="PKM72_12930"
     CDS             1985..2410
                     /gene="exbB"
                     /locus_tag="PKM72_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HNP61741.1"
                     /translation="MDILHQLIDYGIIGLLIALSFWAVAVAIERWLFYRQVDPRKYPD
                     QQTFEVALTKRLVIIGTVAANAPYIGLLGTVLGIMLTFHNMGTSGTMAVSTIMVGLSL
                     ALKATAIGLLVAIPCVVLNNVLRRRVAELLSLYKVQHGA"
     gene            2400..2774
                     /locus_tag="PKM72_12935"
     CDS             2400..2774
                     /locus_tag="PKM72_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNP61742.1"
                     /translation="MERELNQINVIPLVDVMLVLLVIVMTTATFITTGQIPVDLAKAK
                     EAGTKKDTPLVVTLTAEGEIFLNDRPMTRPDLLLALQSHSKDSLILVRADRVIILERF
                     VEVVDEVKGLGFEHISLEVIRS"
     gene            2771..3910
                     /locus_tag="PKM72_12940"
     CDS             2771..3910
                     /locus_tag="PKM72_12940"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNP61743.1"
                     /translation="MTLATLEALSVAPDHCRVQAWAGSTFVHILFVSLAILLITDLQP
                     TSQSEPLKLDMAFIKPAPPKIHNQPEPQELPKPTPVHQQVVPEKVPLPTPVQQPMMKR
                     EPVIEEHKPLPTKKTQEVLHTDTVPMPRQEQTPEPVQAKPILESTPIEQEVVSEPAKH
                     ILSPSTHSKSVEQVKPVMQRETSLTQQAEPSPQALPTPVAEVRERVDSPMQTTLVAER
                     VTESVPTTRPEASVQSQAIQTKAEVVEPTVPIAQEKKIARERPAPQIHQRTAAVERPI
                     QSYPEAQADYGWLAQTIWHQIEKYKSYPKKARQKEWEGKVVLEAVIRQDGTILHLRVA
                     ETSGHEILDKDALSLLWKLSPLTLDHALGHPQLTILVPLTYKLDG"
     gene            4003..>4250
                     /locus_tag="PKM72_12945"
     CDS             4003..>4250
                     /locus_tag="PKM72_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61744.1"
                     /translation="MCNKKMNRRLTNPYVVLLFGTFLCVAAPVAWSASESGESFRAVA
                     TDTEGLQTEMDNIIFYYEEKVSETSFIPHELRELPVKRG"
BASE COUNT         1185 a         1115 c          944 g         1006 t
ORIGIN      
        1 atcagttgtt gaagcttctc gaccgaacaa ccaagacacc gcattctcct ttggtagcga
       61 gaaaattttc attgatttat ttgaaacagt attgaacgta aaacgcagga ttcataagta
      121 tgttccgaaa aagtccgagt tctcggaatc atattgacag catctgatac ataatgatat
      181 ttattttcaa tatcaaaaaa tatcatacat tcatcctaaa ttaataaaaa ctatctttta
      241 ccattagatc ttaaataaga ggattctgtt atttaaatat caccaaggca gacaatccct
      301 cattcattga aggaattaga ggctagaagc ccatgaggct agagcccaaa ggtccatata
      361 cctttgggct ctgcttgttt ttaggcattg ggccttgagt gataagtggg gagaacgaca
      421 gtcagccgcg atgcttttcc tccgttcatc ccagggaaga agcattgggc aaaatcccta
      481 tagccctctt cctaatggtt aaagcatgaa agtcagagca tgcttaaagc cacttgggtt
      541 gctgagaagc cgttttccaa tcaaattcgg caacctgcca agcaccattc ctataagcgc
      601 ttcagatcct ctcaggcgct tgcaggttct tttattttgc acgccctgtt cttatcgatt
      661 tgttattctg tggcctttca agccagcgaa ccaaaaaggg aaattgaact ttccacgttt
      721 cgttggaatg tttcactgac caccgctccc atcccggagc ctgtgacatc cgataccccg
      781 tctcccttgc tttcagcgcc acctcaagaa accgtcgtag cccattctga atcctcgcac
      841 cacttgacgc gtaacgcgtc aagcaaacac tcacgctcaa cattagaagg cggtcggaag
      901 aagctttcga cagatttatc cgtccaatcc aaaacgatcg ccttcccctt aaccgaattc
      961 cctgtcaaac aatctctgaa taccgaaaaa caggcccgac caccatcaaa aacaaccacc
     1021 gcacactctc aaccatcgac tcaagagcag atcaacacgg tgccttttac ccatggccaa
     1081 accgcaaaat taccgccacc ggttaaaaga gtcgtagacc gggctgagcg agtaatgcaa
     1141 ccgagtgccc agagagaacc gaagataatt catcggacac gggtatttca cccaccgatt
     1201 cagagacggg agacacgacc cgattatggg tggttaatca cagatctgag gaaaacatta
     1261 gaacgattaa agttctaccc cgaaattgca cggttaaata aatgggaggg aaaagtcctg
     1321 gtccagatga aagtccttga aggtggtcat ctgatcgaca tggaaatcca ggaaagttcg
     1381 ggatttgagg tgctcgacca aaccgctttg acgatcgtac gtcaggcatc cccgctggat
     1441 ttgggtcacc ctcttccgtc aaacagtgtc agtttatccg tacctctgac ttttcaattg
     1501 gattgacgag tcttttttag cgatccgggg tgaatgtatc catcgaggcc tttcatcatc
     1561 aaatggcacc gactggtgag caagcaaagg caacaaaaca aggggatacc ttcaacctcg
     1621 aggattggac atcagtgtca ataaaattca ttaaaaatat tggtcgggca tgtccggttt
     1681 ttccacgttc atacgtctaa tcctagtatg acgatatctt cattattaaa taaatatcct
     1741 cagttgcggt gttgggcatg attgacgcgt tttcctttca tggacgagtc gaacacgaca
     1801 tggtcagatt cgaccgatcc aagtcctata tgtcatctcg tcttctctgc caaagagttc
     1861 atgcagtccc atcacgggat tcccgcaaaa ggccctacct agcgtaacac aaacccattt
     1921 cccataaacc caacgaggta ccaccgcgaa ggtcacgaac cttaggaagt caggaaaaac
     1981 actcatggac attttgcatc aactgattga ttacggaatt attgggcttt tgatcgcctt
     2041 aagtttttgg gctgtcgctg ttgccattga acgctggttg ttttaccggc aggtcgatcc
     2101 ccgaaaatat cctgaccaac aaacgtttga ggtagctctg actaaacggt tggtgatcat
     2161 cggaaccgtc gcagccaatg ctccgtatat cggcttattg ggaactgtgt tgggtatcat
     2221 gctgaccttt cataacatgg gaacctccgg cacgatggcc gtcagtacca tcatggtcgg
     2281 attgagtctg gcattgaaag cgacggccat cggattgctg gtcgccattc cctgcgtagt
     2341 tctgaacaat gtgctacgcc gccgagtggc cgaattactc tctttgtaca aagtgcaaca
     2401 tggagcgtga actcaatcaa atcaacgtca tccccctggt ggatgtgatg ttagtgctat
     2461 tggtcatcgt gatgaccact gcgacgttta ttacaactgg tcaaatccct gtagaccttg
     2521 ccaaagccaa agaagccgga accaaaaaag atacgccttt ggttgtgact ctgacagcag
     2581 agggagagat ttttctgaat gaccggccga tgacccgccc ggacctgctc cttgcgctcc
     2641 aatctcactc caaggactct ctcatactcg tgcgtgccga tcgagtcatt atcttagagc
     2701 ggttcgtcga ggtcgtggac gaggtgaaag gattaggatt tgagcatatt agccttgagg
     2761 tcattcgatc atgaccctcg ccacgttaga agctttaagt gtggctccgg accattgccg
     2821 ggttcaagcc tgggcagggt ccacctttgt tcacatatta tttgtcagtc tcgccattct
     2881 cctcattaca gatctgcaac cgacgtcgca atcagaaccg ttgaaattgg acatggcctt
     2941 catcaaaccg gctcctccca aaatccacaa ccaaccggaa ccccaggaac tgcccaaacc
     3001 gacgcccgtt caccaacaag tcgtaccgga gaaagtgcct ctcccaacac ccgtccagca
     3061 accgatgatg aaaagagagc ccgtcatcga agaacacaaa cccctgccta ccaagaaaac
     3121 ccaggaggtc ctccataccg acaccgttcc aatgcccaga caggaacaaa ctccagaacc
     3181 ggtgcaagcc aagccgatcc tggaatcgac gccgattgaa caggaggtcg tctcggagcc
     3241 tgccaaacac attctttcgc catccaccca ttcaaaatcc gttgaacagg tgaaaccggt
     3301 catgcaacga gagaccagtc tcactcagca ggcggagccc tctcctcaag cactccctac
     3361 ccccgtagcc gaggtcaggg aaagggttga ttctcccatg cagaccaccc tggtcgccga
     3421 acgggtgacc gaatccgtac ccacgacgcg ccctgaggct tcagtgcaat cccaggcaat
     3481 tcagacgaaa gcggaagtag tcgaaccgac cgtgccgata gcccaagaaa agaagattgc
     3541 ccgtgaacgc ccagcaccac agattcatca gcgaaccgct gcggtggaac gccccatcca
     3601 gtcgtatcct gaggctcagg cagattatgg gtggttagca caaactatct ggcatcaaat
     3661 tgagaaatac aaaagctatc cgaagaaagc gcgtcagaag gaatgggaag gaaaagtcgt
     3721 gctggaagcc gtcattcgcc aggacggcac aattttacat ctccgggtgg cagaaacgtc
     3781 agggcatgaa attcttgata aggatgcctt gagcctgctg tggaaacttt ctccgttgac
     3841 acttgatcat gcattagggc acccgcaatt aaccatttta gtgcctctga cctataaact
     3901 cgatggatag caacaagagg aatccaatgc atccctgata tccgacatac caagacaacc
     3961 attcatccca caaccaaaga atttaaccta aaggagtatg cgatgtgtaa caaaaaaatg
     4021 aatcgaaggt tgaccaaccc atacgttgtt ctgttgttcg gaaccttttt atgtgttgcg
     4081 gcaccggtag catggtccgc ctccgagtcc ggagaatcct tcagggccgt ggcaactgat
     4141 acagaaggtc ttcagacgga gatggacaac attatctttt attatgaaga aaaagtcagt
     4201 gaaacgtcct ttattcccca cgaacttcgg gaattaccgg tcaagcgagg
//
LOCUS       DAORAQ010000217         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2958_length_4242_cov_68.837593, whole genome shotgun sequence.
ACCESSION   DAORAQ010000217 DAORAQ010000000
VERSION     DAORAQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2958_length_4242_cov_68.837593"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKM72_12950"
     CDS             <1..515
                     /locus_tag="PKM72_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP61745.1"
                     /translation="VAATNRDLMQMVEAGEFRSDLYYRLNVFPITVPPLRERQEDIPI
                     LVRYFAQRYATRMKKPIETIPAKTMERLEGYPWPGNIRELENLIERAVILSQGSELAV
                     PLLDLKAGLQRGTQSGATLEGAERDHILRALQDTKWVIGGPTGTAARLGMKRTTLISK
                     MKKLGISRPT"
     gene            632..1729
                     /locus_tag="PKM72_12955"
     CDS             632..1729
                     /locus_tag="PKM72_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNP61746.1"
                     /translation="MFQRFSINRLPWRGGLGPLLITVLFLPGAPGALAANAQPRESCK
                     VVSTPYQLKIPFGLEDEIKPFIPADNPLTVEKVELGKLLFFDPRLSRDNTMSCASCHK
                     PELAWTDGTKLSMGIDNQVATRNGMTVVNRLYGHAQLWHGKMATLEAQAVNPLTKAVR
                     MGMPSTDAEVEKLNAIKGYRERFQQVFCTDVTIDGVAKAIAAFERTLLSGNSPADRFD
                     MGGEEDAISESAKRGLQVFQGKGRCTRCHSGFNFADEEFHNLGIDWDTAKADLGRYSV
                     EKHPGTVGGFKTPTLREIARTAPYMHDGRFATLEDVVDFYDQGGIQNPHLSNLIIPLG
                     LTDQEKKDLVEYMRALNGEGWQMTAPTQFPQ"
     gene            1868..2308
                     /locus_tag="PKM72_12960"
     CDS             1868..2308
                     /locus_tag="PKM72_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNP61747.1"
                     /translation="MQGFLDHIVLNIEHDESMIDFYTRILMLPAERLEDYRAGKAPFS
                     SVRINRDTVIDLFPKKLWEQNISAGTGFSNLNHFCLSLTKTDWEDLCGRLRDNNIAIS
                     EGPVQRWGARGTGTSIYFLDPEGNAIEARFYEVNDQPEKCLLGT"
     gene            2858..3352
                     /locus_tag="PKM72_12965"
     CDS             2858..3352
                     /locus_tag="PKM72_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNP61748.1"
                     /translation="MKTLFQTEESWAPVILRVMLALVIFPHGAQKLLGWYGGNGFEGT
                     MGFFTEQMGLPWVIAFLVIMGESVGALAMAAGFMTRFTAASLGIIMLGAITTVHWSNG
                     FFMNWFGQQAGEGFEYHLLVIGMGLSLMVTGAGKWSVDQVIGKWLARQESLHADGTRR
                     TAAA"
     gene            3474..4019
                     /locus_tag="PKM72_12970"
     CDS             3474..4019
                     /locus_tag="PKM72_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61749.1"
                     /translation="MNRTMNLHVIMLSAVMGMFLIMSGIGYADSKSVSHGHKQVTGVV
                     TTEKGGVLTVKTPTGDYSLTENASHRHGHAVPKVGDEVTLVLDENNTVIEAHPKGEEG
                     IHQFYTGKLVYMGKMNKEIKLQTPDGEKVFPLGRLEIKTKPIEEGAMVTVEVNEGGTV
                     IDLHRASHDETSINAKGKTHE"
BASE COUNT         1093 a         1011 c         1132 g         1006 t
ORIGIN      
        1 tggtcgcggc caccaaccgc gatttaatgc agatggtgga agccggcgag tttcgcagtg
       61 acttgtatta ccggttgaat gtctttccca tcacagtccc gccccttcgc gaacggcagg
      121 aggatatccc gattcttgtt cggtattttg cccaacggta tgccaccagg atgaaaaaac
      181 ccatcgagac cattcctgcc aagacgatgg aacgtctgga aggctatccc tggccgggga
      241 atattcgcga actggaaaac ctgattgagc gcgcggttat tctttcccag ggttccgaac
      301 tggctgtgcc gttattagac ctcaaagccg gtcttcagcg agggactcag tctggtgcga
      361 ccttagaagg tgcggaacgg gaccatattc tccgtgccct gcaagacacc aaatgggtaa
      421 tcgggggccc tacaggcacc gcggcccggc tcggaatgaa acgcaccacg ttgatctcga
      481 agatgaaaaa actcggaatc tcccgcccga cctaatatgg tgtgttttct gcaaattctc
      541 tccaactttt catgtgagtg actatccccg gtctcctgct cgaagaaaaa agctctggcc
      601 aaacatgttt cattcaatct ggaggttcat catgtttcag cgattctcaa tcaatcgcct
      661 cccatggaga gggggcttgg gtcctctcct catcacggtc ctatttcttc ctggagcgcc
      721 aggggctctc gctgccaatg cacaacctcg tgagtcgtgc aaagttgtca gtaccccata
      781 tcaattgaaa attccgttcg gtctggagga tgagatcaag ccatttattc cggcagacaa
      841 tccgctgacg gtcgaaaagg tcgaactcgg caagctgtta ttcttcgatc cacgcctgtc
      901 acgtgacaat acgatgtcct gcgcgagctg ccacaaaccc gagctggcct ggactgacgg
      961 caccaagctc tccatgggta tcgataacca ggtggccacc cggaacggaa tgactgtcgt
     1021 caaccgactg tacggccatg cccagctctg gcatgggaaa atggccacgt tggaagcgca
     1081 agcggtcaat cccctcacca aggcggtacg gatgggaatg ccgtccacgg atgcggaggt
     1141 ggagaagctc aatgccatca agggataccg ggagcgattt cagcaggttt tctgcacgga
     1201 tgtcaccatt gatggggtgg ccaaagcgat tgcggcgttt gaacggactc ttttgtccgg
     1261 caacagtccg gcagatcgat tcgacatggg cggagaagag gacgcgatct ccgagtccgc
     1321 caaacgaggg ctccaagtgt ttcagggcaa gggccgctgc acacgctgcc attcgggatt
     1381 caatttcgcc gacgaagaat ttcacaatct cggcatcgat tgggatacgg cgaaagccga
     1441 tctgggacgg tattcggtgg agaagcatcc ggggaccgtt ggcggattca aaacgccaac
     1501 cctacgggag attgccagga ccgctccgta catgcatgac gggcgtttcg ccacccttga
     1561 agacgtcgtc gatttttacg accaaggggg aatccagaac ccgcatctgt ctaatttgat
     1621 tattcctttg ggattaaccg accaggaaaa gaaggatctg gtggaataca tgcgggctct
     1681 caatggtgaa ggatggcaga tgaccgcgcc aacacaattt ccacaatgac gcagcgttct
     1741 agagaccagg ggcgtattca cgcccgtatt acccttcacc ctctcgcctc taaaaaggtc
     1801 cttgggctgt gcagatccgg gaacgtgttg tacgtaccaa cagcttttaa agatggagca
     1861 ttcgcaaatg caaggatttc tcgatcatat tgtcctgaat attgaacatg atgaatcgat
     1921 gatcgatttt tataccagaa ttttgatgct accggcagag cgactcgaag actatcgtgc
     1981 cgggaaagcg ccgttttctt ctgtgcggat caatcgtgat accgtcatcg atttatttcc
     2041 caagaagtta tgggagcaaa acatttcagc agggacaggc ttcagcaatc tgaaccactt
     2101 ttgtctttca ctcaccaaaa cggattggga agacttatgc ggtcgacttc gtgacaataa
     2161 tattgcaatc agtgaagggc ctgtccaacg gtggggagcg cggggaacag gaacctccat
     2221 ttattttctc gatcctgaag gaaatgcgat cgaggcacga ttttatgaag taaatgatca
     2281 gccagagaaa tgcctgcttg gcacttaagg aagtcgttgc caatctctca tagcaagact
     2341 ctcaataaac aatctcggac atgcccaagg gtctcctcct ctgtcatcct ctcagttcgt
     2401 cagcggaggt ccaattggaa cgacaccaaa atggattccc gatgactact gtcggaaatg
     2461 tccgcgatgg agggattcac gagacatggc atcccgagta ctttgccaaa ctgctcgatg
     2521 gatagtccgc ctgccttccg tcatcccgaa tgagagaagc gaaggtatcg ggaatttcat
     2581 gaaaatccct tctacctaac catcttccgt tcgtctgtct gtcgccccct cgacacgagt
     2641 cgacatatcg tcgcatttga ttcgacacac ctcttctctt tcttttgcat cttcgataga
     2701 aatatcaatt cattcaactg tttgtgcatt tttttgatct tttgtgaagg tggaacggct
     2761 cttgccatat gtaagaggta agagttcaac aaagctttcg ttagaacaaa ttatgacaaa
     2821 ccacacacat ttattcattc acaaaggaga ggaagccatg aaaacattat ttcaaacaga
     2881 agagagttgg gcgccggtta ttttgagagt gatgctggcc ttggtcattt ttcctcacgg
     2941 cgctcagaag ctgctgggat ggtatggagg aaacgggttt gaaggcacga tgggattctt
     3001 tactgagcaa atgggtttgc cttgggtgat tgcatttctg gtcattatgg gagaatcggt
     3061 tggcgcactg gcaatggcag ccggattcat gacgcggttc actgccgcga gtttaggcat
     3121 catcatgtta ggcgccatca caacggtgca ctggtctaat gggtttttta tgaactggtt
     3181 tggccaacaa gccggtgagg gatttgaata tcacctgctc gtcattggca tgggtctgtc
     3241 gttaatggtg acgggcgccg gcaaatggtc ggtcgatcag gtcattggaa agtggcttgc
     3301 acggcaggaa tcattgcacg ctgatgggac tcgtcggact gcggcagcct aagtgtgaca
     3361 gatctaaatg tacgactaga aaatgaataa ttatggaaag gagtcaagac atgaataggc
     3421 aacttcgtca ggatagaagc agggatctct caataaccat aaaggaggaa gtgatgaacc
     3481 gtacaatgaa tcttcacgtg attatgttat cagcggtgat gggcatgttt cttatcatgt
     3541 ccggtatcgg ttacgcagac tctaagagtg tcagccatgg acataagcag gtcaccggag
     3601 tcgtgacaac ggagaagggt ggtgttctca cggttaaaac acccacggga gactattctc
     3661 tgaccgaaaa tgcttctcat cgtcatggcc atgccgtccc caaggtggga gatgaagtaa
     3721 ccttggttct ggatgagaac aatactgtca tagaagccca ccctaaaggc gaggaaggaa
     3781 tacatcagtt ctatacgggg aaattggtgt acatggggaa aatgaataaa gagatcaaat
     3841 tgcagacccc tgatggggaa aaggtcttcc ctcttggtcg gctggaaatc aaaaccaagc
     3901 ccatcgaaga aggtgccatg gtcacggttg aagtaaatga aggcggcact gtgatcgacc
     3961 ttcatcgtgc gtcccatgac gaaacaagca ttaatgctaa agggaagact cacgagtaac
     4021 gactcgaatg ttcataatga tgtggctcga tctcttgccg ggtggtgaga gatcgggctc
     4081 atcttctgat aaagaagaca gtgggatgag aaaaaggcaa agagacaacg aaggagccga
     4141 aagatgatat cccttaacgg aaaagttgtc atgatcacgg gggcattggg tggtttaggc
     4201 cagacggtga ccgaggcatt cgcccaggcg ggagccaagg tg
//
LOCUS       DAORAQ010000218         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2968_length_4231_cov_62.136734, whole genome shotgun sequence.
ACCESSION   DAORAQ010000218 DAORAQ010000000
VERSION     DAORAQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_4231_cov_62.136734"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="lgt"
                     /locus_tag="PKM72_12975"
     CDS             complement(<1..722)
                     /gene="lgt"
                     /locus_tag="PKM72_12975"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNP61750.1"
                     /translation="MMAFDPVMLLADLARLPYPDIDPVFFRMGPLALRWYGLMYLLGL
                     AGAYWLIKSRAATRNVSLPPQQLSDLIVYAAFGVFLGGRLGYVLFYNFPFYLDHPLKV
                     FAVWEGGMSFHGGLIGTCIAIWIFCKTRGFPVWPIADLAAGAAPIGLGFGRMGNFING
                     ELFGRSTDVEWCMVFPHGGPECRHPSQLYEAGLEGVTLFVILTILNRWPTPPGTMFWT
                     FICGYGVARFFVEFFREPDSHLG"
     gene            1068..2447
                     /locus_tag="PKM72_12980"
     CDS             1068..2447
                     /locus_tag="PKM72_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP61751.1"
                     /translation="MDEIGSSSRSNGAEIILDCISDGVITIDLQKRVTFLNRAMQKML
                     GYKVDAAGTLLACDVLIQSNICSTKECVLERALQGERVSNFEAVVRRRDGVHIPVSIN
                     TDFLLDKEGKLIGLIEVIRDISLFRELSAKVEEVSQLKHRLGEQTKLDNMVGRCPRMQ
                     DIMAKLPIIAASKSSVLITGESGTGKELIACALHAHSPRKHAPFVVVNCSSLSEGILE
                     SEIFGHVKGAFTNAYFDKPGRFEIANGGTIFLDEIGEMSVATQVKLLGVLERGQFERV
                     GSNDTVTVDVRVVAATNRNLEEAVQQGRFREDLYFRIRVIPMELPPLRERTGDIPLLV
                     NHLLEKFNREMGKHVISLSPQCLTALSQYPFPGNIRELQNMIEHAFVCCEEDTIQFEH
                     LPADLHRYCWEHREWTDSETLEALERQAICRALEKSGWRLKEASQQLGIGRSTLWRKV
                     KQFGITQVI"
     gene            2713..2916
                     /locus_tag="PKM72_12985"
     CDS             2713..2916
                     /locus_tag="PKM72_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61752.1"
                     /translation="MDCPRCQGIMIQDKFGDVADESGAMYFSGWRCITCGEILDPVIS
                     ENRRHHHEPLIGRSRKKFATQLG"
     gene            3115..4011
                     /locus_tag="PKM72_12990"
     CDS             3115..4011
                     /locus_tag="PKM72_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNP61753.1"
                     /translation="MAAEQNPAGPATPPNQPEGNPLPGVKHIIAVSSGKGGVGKSTVT
                     VNLAVALKQQGYAVGLMDADIYGPNIPMMIGVMKEPGKEGEKILPAEGQGVKVISMGF
                     FVPEDTPVVWRGPMVHSAIQQFFRDVVWGELDYLLIDLPPGTGDVPLTLSQLVPLTGA
                     ITVTTPQEVALQDVRKGMTMFKKVNVPLLGVIENMSYFVCGHCHERTEIFSTGGGERA
                     AQKFEIPFLGRIPIDPAIREGGDKGTPIVSNDPSSPQAQAFLQIAKSLTANIDNAEKG
                     NGDAAPSLSSLLKKITDPLKKS"
     gene            4044..>4231
                     /locus_tag="PKM72_12995"
     CDS             4044..>4231
                     /locus_tag="PKM72_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNP61754.1"
                     /translation="MSELVRIAKIEEVEEGKGMVVEAGEKCLAVFKVDGAFHVIDNTC
                     LHRGGPLGEGDVEGETVT"
BASE COUNT         1121 a          887 c         1160 g         1063 t
ORIGIN      
        1 cctaaatgac tatccggttc gcgaaaaaat tccacaaaaa atcgggcaac tccatatccg
       61 cagatgaagg tccaaaacat ggttcccggt ggggttggcc agcgattgag gatcgttaat
      121 atgacaaaga gtgtcacgcc ttccaggcct gcctcataga gttgcgatgg gtgtcggcat
      181 tccgggccgc catggggaaa taccatgcac cattcgacgt cggtcgaccg cccgaataat
      241 tctccattga taaaattccc catccgtcca aaccccaacc cgattggagc cgctcctgct
      301 gccaggtcgg cgatgggcca aacaggaaac ccacgggttt tgcaaaagat ccagatagcg
      361 atacaggtcc cgatcagccc gccatgaaat gacattcccc cttcccatac cgcaaaaact
      421 ttgaggggat ggtcaagata aaatggaaag ttataaaaga gcacatatcc caagcgccca
      481 ccgagaaaaa caccaaaggc ggcatagaca attagatcgg ataattgttg aggtgggaga
      541 gagacattcc ttgtggcagc tctggacttg atgagccaat aggcccctgc caaccccagg
      601 aggtacatga ggccatacca tcgaagagcc aggggtccca ttcgaaaaaa gaccggatca
      661 atgtccgggt atgggagtcg agcaaggtca gccaaaagca tgacagggtc gaaggccatc
      721 atgcgttaac gtgccttccc ccaggaccga tgtgcccgga acaagtgcat gctctaaata
      781 aagaagaatc cgaaattagt cgccaaatgg gaaaatggcg tgcggcattc ttccctcgga
      841 gcgagccggt tggtagaaca tcgttcttct tggaaattgg caagataact atcggcaagg
      901 gtttgtggag cagattaggg ttgccttaca ctaacagaaa aacaggagaa gggtctggtc
      961 aattgttctg aagacctcac cacatctacc taccataaaa tgcagatgaa agaaaagcat
     1021 gttggttgta accggaagtc tttatcttga cgagaggcat agacgtcatg gatgaaattg
     1081 gatcatcgtc ccgttcaaat ggtgcagaaa tcattctgga ttgcataagt gacggggtga
     1141 taacgattga cctgcagaaa cgggtgacct ttttgaaccg ggcgatgcag aaaatgctgg
     1201 ggtacaaagt tgatgcagcg gggacactct tagcctgtga tgtgctgatc caaagcaata
     1261 tttgctcgac caaggaatgt gtcctagagc gggcgcttca gggagagcgg gtgtcgaatt
     1321 ttgaagcagt ggttcgtcgt cgtgatggcg tacatattcc ggtgagtatc aataccgact
     1381 ttttattgga caaggaagga aagctgatcg ggttaatcga agtcatcaga gatatctcgc
     1441 tgtttcgtga gttaagcgcc aaggtagaag aggtctcgca gctcaagcat cggctgggag
     1501 agcagaccaa gctggataat atggttggtc gatgtcctcg aatgcaggac attatggcga
     1561 aattgcccat tattgccgct tcgaaatcct ccgtcctcat cactggagaa agcggaacgg
     1621 gcaaggagtt aattgcttgt gcattacatg cccatagccc gagaaaacat gctcccttcg
     1681 tagtggtgaa ttgctcaagt ctatctgagg gaattctgga gagtgaaata ttcggtcatg
     1741 tcaaaggggc gtttaccaat gcctattttg ataaaccggg ccgatttgaa attgccaatg
     1801 gcgggacgat cttccttgat gaaatcgggg agatgagtgt tgccacccaa gtgaagttgt
     1861 tgggcgttct tgaacgggga cagtttgaac gggttggatc caatgacacg gtcacagtgg
     1921 atgtgagggt ggtggcggcc acgaatcgga atctggaaga agctgttcag caaggacgat
     1981 ttcgggaaga tttgtatttt cgtatacggg tgattcccat ggagctgcct ccactgcgcg
     2041 agcgaacggg agatattccc ctactcgtga atcaccttct cgaaaaattt aaccgtgaaa
     2101 tgggcaagca tgtcatcagt ttgtctccgc aatgcttaac ggctctcagt cagtacccct
     2161 ttcccggcaa tatcagggaa cttcaaaata tgattgagca tgcctttgtg tgctgtgaag
     2221 aggatacgat tcaatttgag catcttccag ccgacctcca tcggtattgt tgggaacatc
     2281 gggaatggac cgattccgaa acactggaag ccttggagcg acaagccatt tgccgggcgt
     2341 tggaaaagtc aggttggcgg ttgaaagaag cttctcagca gttaggcatt ggtagatcga
     2401 ccttgtggcg gaaggtcaaa caatttggaa taactcaggt catttaggag tgtttcattt
     2461 tggacttggt tgttacctag ttgtgccatt cgtatttaag tgttatattt tcaatgtctt
     2521 atacattgat gattcgcaca atttagtttt attggtttca ttttgaaaca ttgaggaaag
     2581 cgggaaatat agaaagtctt tattaattaa gtgcttgtgg ggaatatatt acggcatatg
     2641 gattgctttt ctaattaact cagatacgca ttgatattat tattcttttt tcagtgagaa
     2701 ggagtgcttc atatggattg tccacgttgt cagggaataa tgattcaaga taagtttggt
     2761 gatgtggctg atgaatccgg cgccatgtat ttttcgggtt ggcggtgtat tacctgtggt
     2821 gaaatcttgg atcccgtgat ttctgaaaat cgtcggcatc atcatgaacc cctcatcggg
     2881 cgatcgcgta aaaagtttgc cacgcagcta gggtaggtgt tactgccctt cgtatttctg
     2941 ttcgtacccc cctattgtca ttgtgtgtct gttgagaaag gcccgaaact ctcttccttg
     3001 agggtttcgg gttctctttg attcatgttc taatggtgag tgagccaaaa gatcgaaaaa
     3061 agcctcgcac atgggaaaat tttatgagtg tctgtgaaat aaggagaatg tcgaatggct
     3121 gctgaacaaa accccgctgg tcccgcgacc ccacccaatc aacctgaagg aaatccattg
     3181 ccgggcgtga aacacatcat cgcggtgagc agtggcaagg gcggcgtggg caaatccacg
     3241 gtcacggtga atttggcggt cgcgctgaag cagcagggct acgcggtagg gttaatggat
     3301 gcggatattt atggaccgaa tatccctatg atgatcggcg tgatgaaaga gccggggaaa
     3361 gaaggggaga aaattctgcc tgccgagggg cagggagtca aagtcatttc catgggattt
     3421 tttgttccgg aagacacgcc tgtggtgtgg cggggaccca tggtccattc ggcaatccag
     3481 caatttttcc gtgatgtggt atggggggaa cttgattatt tactgatcga tttgccccct
     3541 ggtacgggag atgtgccgtt aacgttatcc caattagtcc cgttgaccgg agccattact
     3601 gtgaccactc cgcaagaagt ggctcttcag gatgtgagaa aaggcatgac gatgttcaaa
     3661 aaagttaatg tcccgttgct cggcgtgatt gaaaatatga gttattttgt gtgtgggcat
     3721 tgtcacgaac ggacagaaat tttttcgaca ggaggaggcg agcgcgctgc tcaaaaattt
     3781 gagattccat ttttgggacg gattccgatt gatccggcca ttcgggaggg aggagacaaa
     3841 ggcaccccga ttgtgagcaa tgatccctcg tcccctcaag ctcaggcgtt tcttcagatt
     3901 gcaaaatccc ttacagccaa tatcgacaat gcggaaaaag gaaacggcga tgctgcgccc
     3961 tccttatcca gtttactcaa aaaaattaca gatcccttga agaaatctta agtggacagg
     4021 ctctttggca ttgaggagga cctatgtcgg agttagtgcg tatcgcgaaa atagaggaag
     4081 ttgaagaggg aaaaggcatg gtggtggagg cgggtgaaaa atgtttggcc gtctttaaag
     4141 tggatggagc ctttcatgtc atcgataaca cttgtcttca taggggcggt cctttaggcg
     4201 agggcgatgt cgaaggggag acggtgacct g
//
LOCUS       DAORAQ010000219         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2993_length_4183_cov_74.655766, whole genome shotgun sequence.
ACCESSION   DAORAQ010000219 DAORAQ010000000
VERSION     DAORAQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_4183_cov_74.655766"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..850
                     /locus_tag="PKM72_13000"
     CDS             77..850
                     /locus_tag="PKM72_13000"
                     /inference="COORDINATES: protein motif:HMM:NF039455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HNP61755.1"
                     /translation="MSNYIRSDLLALNPHQTVSIQRVGLGQHQVVIVDDFYQYPEEIL
                     KIALNLPYTDRFELVGNFPGVRARLNYEHRELVESLSALWGCPLFTFFSPQPVVFQGI
                     KTDNYRLNVGQRQPHIDQDVTAMVYLNPADSCAGGTGLYRHQPTGLERVPPIPDATIR
                     QLANQLELSDEFFTSPEGYENFQNSMIFNPLFACRDNRYINDGNEYWELLKLIEMRPN
                     RLMMFDGRCFHSQYIQSGHYNQAFRVNQVLYLSQKRKLP"
     gene            complement(1265..2611)
                     /locus_tag="PKM72_13005"
     CDS             complement(1265..2611)
                     /locus_tag="PKM72_13005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HNP61756.1"
                     /translation="MKKHEYRTEPMTTASMPSGVLHLVVNEGAERFSYYGMRSILVVF
                     MTGMLLNTQGSLDPMGEAEAKSYFHLFASAVYFFPFLGAILADAFLGKYKTIILLSLV
                     YCGGHVVLALDHTRFGLLLGLILIAIGSGGIKPCVSANLGDQFGIANAHLLTKAFAWF
                     YFAINVGAFGSVMLTPWLLEHYGPGLAFGVPGILMFVATIIFWSGRTHYVHIPPDRAG
                     VLQALSSPQAWRAIGKLAGIYVFVAFFWSLYDQTASAWVLQAQHMNRVWMGIEWLPSQ
                     IQAVNPVLIILFIPLFTYVVYPVLNRIVLLTSLRKMALGFFMTAGAFLISAYIEQQIE
                     LGMKPAIGWQLLAFVVITAAEVMVSITCLEFSYTQAPLKLKSLLMGLFLLSVSLGNGF
                     TSFVNFWIQNADGTVALTGPDYYWFFAGVMFLVACLFLLVVKGYEEETYLQGTGQG"
     gene            2734..>4183
                     /locus_tag="PKM72_13010"
     CDS             2734..>4183
                     /locus_tag="PKM72_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNP61757.1"
                     /translation="MPTPFLPFPGKILLISLSVIRIWLPTPVQSGESSVPTLDTMIVT
                     GSAQPTQSHQSTQSITTLNAEQISPLQPNRVTTILQQVPGLHTDEMSGRSGISSVYLR
                     GADPNFTLIMLDGVPMNDSTNQRGGSVDLSTIPIDRIERVEIVRGPLSAFYGSEAMAG
                     AINFITKSASTTPSFRLLGEGGRYDYLKGLVQTGGSLGPLSANLTLSHTQNGEQIEKD
                     SFLMDSAGWNFSIQTDPNFDIQLNGQYTDSTVRSFPEGSGGSEFALLRETERRKTQEF
                     LTGLSASFHMPSGWQHQVFLSVTRRLQDVSNPGILATPATYALPPADFETLYTRFQTR
                     MTTTWTINPQWKFSLGEQLTHERGRRNGTQNLSSFGGSSDQPDDFAIRRTLGGTFAEL
                     TSVLWQKLTLNSGVRADISQGFQPKINPRIGAKYQLVDSIQIRGGYGRGFKLPSLSSL
                     GDPLIGNPSLKPETSVGWDLGLDYHTVDERFTA"
BASE COUNT         1183 a         1103 c          909 g          988 t
ORIGIN      
        1 tgactcctct tgaaacaagg ttcactatag gcccgttttt ttaacgtcat cctagtcgaa
       61 taccaatagg gactccatgt ccaattacat tcgctcggat cttctggccc tcaatccaca
      121 ccaaacagta tcaattcaac gcgttggact gggacaacat caggtcgtga tcgtagatga
      181 tttctatcaa taccctgaag aaatcctcaa aatcgcgtta aacctcccct atacggaccg
      241 tttcgaactc gtagggaatt ttcctggagt tcgagcacga ctcaattatg agcaccgaga
      301 attggtagaa tcgctaagcg ccctttgggg atgccctctc ttcacatttt tttctcccca
      361 accagtcgtc tttcaaggta tcaagactga caactaccga ctaaacgttg ggcaacgaca
      421 acctcacatc gatcaggatg tcaccgccat ggtctatctt aatcctgcag attcttgtgc
      481 gggtggaacc gggttatatc gccatcaacc gacaggactc gaacgggtac ctccgatacc
      541 ggacgcgacg atccgacaac ttgcgaacca attggaactg agcgacgaat tttttaccag
      601 cccggaagga tatgaaaact tccaaaatag catgattttc aatccccttt ttgcatgtcg
      661 agacaatagg tatatcaacg acggaaatga atattgggaa ttgctgaaac tcatcgaaat
      721 gcgcccaaac aggttgatga tgtttgatgg tcgatgtttt cattctcagt atatccaatc
      781 cggccactac aaccaggcct ttcgggtgaa tcaagttctg tatcttagtc aaaaaagaaa
      841 attgccttga ttcttcaatg acctgtcttc gattttttct cccgtttttt ttgtagattt
      901 cccctcttcc ttataaagct tgtatctcca tcagatggtt ataggattgt tcgtagggcc
      961 ctggtttaca ggaaggggct gttgaaaaaa gccgccagcg gcgttctcgc catttttccg
     1021 tgctcacgta ctggaagtac gctctgcgcg caaaaatgac tgcggccttg ctggacgact
     1081 tttttgaacc gtcccgaggc ctctaatatg caacgtgcct gtgggtcaat aggcccttgg
     1141 aaattataat ttttcaacat tcacaggaag gaaaagaata ttcccctact cctcacggtt
     1201 ataaggaagg atcgtgagga ttattaatgt ctggatgggc gagagacttg aatctccggt
     1261 cagatcatcc ctgtcctgtt ccctgcaaat acgtttcttc ctcgtaacct tttaccacca
     1321 aaagaaacaa gcaggccaca agaaacatga cgcccgcaaa aaaccaatag tagtcgggac
     1381 cggtcaacgc aacagtccca tctgcattct gaatccagaa gttcacaaag gaggtaaacc
     1441 cgtttcctaa agacaccgaa agcaaaaaca agcccatgag aagcgatttg agtttaagtg
     1501 gagcctgggt ataggaaaat tcgagacagg taatagacac catgacttcc gcagccgtaa
     1561 tcaccacgaa ggccaataac tgccagccaa ttgctggctt catccccaat tcaatctgtt
     1621 gctcaatata ggctgagatc agaaaagccc cagccgtcat aaaaaagccc aaggccatct
     1681 ttctcaatga tgtgaggagg actattctat tcaaaaccgg atagaccaca taggtaaaca
     1741 aaggaataaa cagaatgatc aagacgggat tcactgcctg aatttgggaa ggcaaccatt
     1801 caatacccat ccaaacacgg ttcatgtgtt gggcctgaag aacccatgct gaagccgttt
     1861 ggtcatagag agaccaaaaa aatgcgacaa acacataaat ccccgccaac tttccaatcg
     1921 ctcgccaggc ctgtgggctt gaaagcgctt gaagcacccc tgcccggtcc ggaggaatat
     1981 ggacatagtg agtccggcca gaccaaaaaa tgatggtggc cacaaacatc aagatgccag
     2041 gcaccccaaa ggctagaccc gggccataat gctccaaaag ccagggggtc agcattacag
     2101 acccaaaagc tccgacgttg atggcgaagt aaaaccaggc aaaagccttg gttaaaagat
     2161 gcgcattggc aataccaaac tgatctccca gattcgctga tacacagggc ttaatgcccc
     2221 cggagccgat cgcaatcaaa atcaacccaa gaagtaggcc gaatcgggtg tgatcgagag
     2281 ccaaaacgac gtgcccccca caatacacca aagaaagaag aatgatcgtt ttatatttcc
     2341 ccaaaaaggc atcagccaag atcgctccca aaaacgggaa aaagtacact gccgaggcaa
     2401 acagatggaa ataacttttt gcttcagcct cgcccatggg atccaggctt ccctgggtat
     2461 tcagtaacat cccggtcata aacacgacca gaatggaacg catgccatag taactaaatc
     2521 gctcagcgcc ttcattgaca actaaatgca ggacccccga tggcatggaa gccgtggtca
     2581 tgggttctgt tcgatattca tgttttttca tgaaccaact ttcatctgta agaagtggac
     2641 aaccattgaa aattcgtata gatcctatcc ggtgagaacg gctgtgtcag tccgacaggc
     2701 aaacctccgt ccccaccgga atgtcattct ataatgccga ccccatttct gccattccca
     2761 ggcaaaattc tcttaatcag tctttcagtt ataagaatat ggcttcccac gccggtacag
     2821 tccggggaat catcggttcc gactcttgat acgatgattg tcacaggttc cgcccaaccc
     2881 acccaatctc accaatcaac tcaaagcatt acgaccctca atgccgaaca gatttccccc
     2941 cttcagccca atcgggtaac caccatcctc cagcaagtcc caggactcca taccgatgag
     3001 atgagcggga gaagtggaat cagctcagtg tatctccgag gtgccgatcc caacttcacc
     3061 ttgattatgc tggatggggt ccccatgaat gactcaacca accagcgagg cggatcagtt
     3121 gatctttcca ctattcctat tgatcggatc gagcgagtag aaattgttcg ggggcctctc
     3181 tcggcatttt atggttctga agctatggcg ggcgcaatca atttcattac caaatctgct
     3241 tccacgactc cctcctttcg ccttttgggc gaaggaggtc gatacgatta tctcaaaggg
     3301 cttgtccaaa ccggaggatc acttggacca ttgtccgcca atctgacgct gtcccatacc
     3361 caaaatggag agcaaatcga gaaagattca tttttgatgg actcggctgg atggaatttt
     3421 tccattcaga cagatcccaa cttcgacatt caactcaacg ggcaatatac agattcaacg
     3481 gtacggagtt ttccagaagg cagcggtggt tcggaattcg ctttgctccg ggaaaccgaa
     3541 cgtcgaaaga cccaagaatt tctgaccggt ttgtcagcct catttcacat gccttcgggt
     3601 tggcagcatc aggtatttct gagcgtcact cggagacttc aagacgtgtc aaaccccgga
     3661 attcttgcga ctcccgccac ctatgccctg cctccggctg actttgaaac cctctacacc
     3721 cgttttcaaa cacgcatgac gacaacatgg acgatcaacc cacaatggaa gttttccctt
     3781 ggtgaacagc tgactcatga acgcggaaga cggaacggaa cacaaaactt gagttccttc
     3841 ggaggctcct ccgaccaacc agatgacttt gccattcgcc gaacactcgg tggaaccttt
     3901 gccgaactca catcggtctt gtggcaaaaa ctaaccctga actccggtgt tcgagccgat
     3961 atctcacaag ggttccagcc gaagattaat cccaggattg gagcgaaata tcaactggta
     4021 gactcaatcc agattcgtgg tggctatgga agagggttca aattgccgag cctatccagt
     4081 ttaggagacc ccttaattgg gaacccgtcc ttaaaacccg aaaccagtgt cgggtgggat
     4141 ctcggcctag actatcacac agtcgatgaa agatttaccg caa
//
LOCUS       DAORAQ010000220         4182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_2994_length_4182_cov_63.537194, whole genome shotgun sequence.
ACCESSION   DAORAQ010000220 DAORAQ010000000
VERSION     DAORAQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2994_length_4182_cov_63.537194"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1436)
                     /locus_tag="PKM72_13015"
     CDS             complement(<1..>1436)
                     /locus_tag="PKM72_13015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNP61758.1"
                     /translation="PDKAEYSDKPQFVNSFHVKELYFEEFQELLLNYFGHAQFYGQRV
                     RYGSCIGPLHNASTPPANFRLSQGRAHKFLSIPEPVYFVAIVGNGELPVLPSGIFVPE
                     VPEYWHQHLALQAGIEERDQQLSHLQSQLLSATADHARVLEDRDQRFSNLHARLLTKE
                     EELRCIHSSLLWRNISKVRILRERLCPEGSRRGRACASLVHWLKASGGSGNFNGKSNG
                     GSLDRILEVAKSMVPVSSHRKHQLVSFVFSRFGSFFQQTASYRRWKDMQLPALKESGN
                     LDYLTQDPPRKMPSLIQLGPGGVTALVQSFNFTDVKQPLVSIVIPVYNHLEYTVCCLA
                     SILKHVPQCSFEVIVVDDGSQDETSNILSTIDNIRYCRNEENVGFLRSCNTGASLAKG
                     QYLLFLNNDTQVLEGWLDELVNTFQEQSDVGLVGSKLLYPNGVLQEAGAIIKPDGSAE
                     LVGLNGDPDRPEYNVVREVDYCSGASVL"
     assembly_gap    1437..1536
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1537..1832)
                     /locus_tag="PKM72_13020"
     CDS             complement(<1537..1832)
                     /locus_tag="PKM72_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61759.1"
                     /translation="MKKIEEQVSSGRMPSLPDADQEREVIESDSISLSSTETRQGIMA
                     DVTEPPKLPWTGERYVPGVIGDIELEHLHRYYLARELAVGKDVLDIACGEGYGSA"
     gene            complement(1971..3323)
                     /locus_tag="PKM72_13025"
     CDS             complement(1971..3323)
                     /locus_tag="PKM72_13025"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP61760.1"
                     /translation="MSSELAIRVQGLGKAYQIFRKPEDRLKQMLWRGHRQFYEEFWGL
                     QGVDLSVYRGETVGIIGRNGSGKSTFLQMVCGTLSPTCGDLTVKGRVAALLELGSSFN
                     PEFTGKENVYLAASILGLTSEQIDARYESITEFAAIGDFINHPVKIFSSGMYARLAFS
                     LVAHVDADILIIDEILAVGDAAFTQKCMRFLHQFREKGTLLFVSHDTASVTNLCNRVV
                     WLDNGAVREIGPAKEVCHNFLAALYHEQENANTFRIGGSRKAPTDLAMPVKDPRSEML
                     KNSSHRNELEIFDFDPNAPWFGERGASICEVALSDQHGSLLTTLDGGEEVTLTIRCVS
                     ERPLARPILGFYVKDRLGQYLFGDNTFLMYREVDRSIEKGQRFHAMFRFQLPYLPTGD
                     YSVIAAIAEGTPENHALHHWIDDALFFRVHSSHVLRGLIGIPMLAIEFESSVMPLAPS
                     "
     gene            complement(3313..4128)
                     /locus_tag="PKM72_13030"
     CDS             complement(3313..4128)
                     /locus_tag="PKM72_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP61761.1"
                     /translation="MNNAYNPFSPLICAWEHRTLIARLAKREIDARYRGSMLGVIWAF
                     VVPMLMLGVYTFVFSVVFRIRWEVPMEENGAFALLLFSGLIIFNLFSECVTRAPGLML
                     ENVSYVKKVVFPLEIMPWVSMQVALFNAAVSLLILLVFYLVVHGLPPLTVLLLPVVLF
                     PFVLLIVGLGWFLASVGVFLRDVQPLVGVMATMMMFLSPIFYPLSAIPEAYRGLIQLN
                     PLTPVLNASKSVIFWGQMPEWLDWFLYMVVFWMVGWLGYAWFQKTRKGFADVV"
BASE COUNT         1206 a         1114 c          853 g          909 t
ORIGIN      
        1 atgagcacac tagccccgga gcaataatcg acctcccgca ccacattata ttcaggcctg
       61 tcagggtcac cattcaaccc gacaagttcc gctgaaccat ctggcttaat aatggcccca
      121 gcctcctgaa gcacaccatt gggatacagc aattttgagc ccacaaggcc aacatccgat
      181 tgttcttgaa acgtgtttac taattcatct aaccatcctt caagcacctg tgtatcatta
      241 ttcagaaaca gcagatattg gcctttggcc aatgacgcgc ctgtattgca cgaacgcaga
      301 aacccaacgt tttcctcatt ccgacaataa cggatattat ctatggttga gagaatattt
      361 gaagtctcat cctgagatcc atcgtcaacg acgatcacct caaaactaca ctgaggcaca
      421 tgtttcaaaa tagaagccaa acaacagacg gtgtattcca agtggttata gacagggatc
      481 acaatggaca ccaaaggctg cttcacatca gtgaaattga atgactggac gagcgctgtc
      541 accccacccg gcccaagctg aatgagagaa ggcatcttgc gaggagggtc ctgagtaagg
      601 taatccagat ttccagactc cttaagcgca ggcaattgca tgtccttcca ccgccgataa
      661 ctcgccgtct gttggaaaaa ggagccaaac cgtgaaaaga caaacgacac caattgatgt
      721 tttcgatgag aagaaacggg caccatcgat tttgcaacct cgagtattcg atcaagcgac
      781 ccaccgtttg atttcccatt aaaattgcca gaaccacctg acgcctttaa ccaatgcaca
      841 agactggcac aggcccggcc acgacgtgag ccctcaggac aaagccgttc tctcaaaatc
      901 cttactttgc tgatgtttcg ccatagcaag gaggaatgta tgcaacgtaa ttcttcctct
      961 ttcgtcagaa gtcgggcatg caggttgcta aagcgctgat cccggtcctc aaggacacgt
     1021 gcatggtcag ccgtggcact caacaactga ctttgaagat ggctaagctg ttgatcgcgt
     1081 tcttcaatgc cagcctgcag cgccaggtgt tgatgccaat actctggaac ttccggaaca
     1141 aatatcccag aaggaaggac gggtaactct ccgttcccga cgatggccac gaaatagaca
     1201 ggctcaggaa tactcaaaaa cttatgggct cgcccttggg ataatcgaaa gtttgccggc
     1261 ggcgttgaag cgttatgaag cggaccaata catgacccat atcgcactcg ctggccataa
     1321 aactgcgcat gcccgaaata attcaataac agctcttgga actcttcgaa atagagttcc
     1381 ttcacatgaa aggaattgac gaactgtggc ttatcggaat attcagcctt atccggnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg aaccataccc ctccccacat
     1561 gcgatatcga gcacatcctt ccctaccgcc agttcacggg caaggtaata gcgatgcaaa
     1621 tgctctagct caatatctcc aatgactccc ggaacatagc gttcgcctgt ccacggaagc
     1681 tttggcggtt ctgtcacatc ggccataatg ccctgccttg tttcagtgga ggacagagag
     1741 attgaatcgg attcaatgac ctccctttcc tgatctgcgt cgggtaacga tggcatcctc
     1801 ccggaggaga cctgttcctc aatctttttc atgttttggc tctatacatc attgatcacc
     1861 accaagccag ggttaaatta gcaccttcac ccccatcccc ccaaagcctc attccggccg
     1921 gccaggaacc acacagaaca ctttaaacct gaccgtgtat ccctgagttc tcaggacggg
     1981 gccaagggca tcaccgaaga ttcaaattca atcgccaaca ttggaatgcc aataagccct
     2041 cgtaggacgt gacttgagtg aactcgaaaa aacagggcat catcgatcca atgatgaaga
     2101 gcgtgattct ctggtgtccc ctcagctatc gccgcgatca ccgaataatc accggttggg
     2161 agatatggta gctggaaacg aaacatcgcg tgaaatcgtt gccccttctc aatggagcgg
     2221 tcaacttcac gatacatcag aaacgtattg tcgccaaaca aatactggcc tagtcgatct
     2281 ttcacgtaaa acccaaggat gggtcgcgct aaaggtcgtt ccgacacaca ccggatggtc
     2341 aacgtgactt cctctccgcc gtctagcgtt gtcaggagag agccatgttg atcggaaagc
     2401 gccacttcgc aaatactggc tccccgctca ccaaaccacg gagcatttgg atcgaaatcg
     2461 aaaatctcca actcatttcg atgtgaagag ttcttcaaca tttcactccg tggatctttt
     2521 accggcatgg ctaaatctgt tggagctttg cggcttccac caatacgaaa ggtattggca
     2581 ttttcctgtt cgtgatacag cgcagcaagg aaattatgac agacctcttt tgcgggacca
     2641 atttccctga cggccccatt atcaagccaa acaacccggt tgcaaagatt ggtcaccgaa
     2701 gccgtatcat gggagacaaa caggagcgtg cctttttccc gaaattgatg aaggaaacgc
     2761 atacattttt gagtaaaggc ggcatcaccc acggcaagga tttcatcgat aatcagaata
     2821 tcggcatcca catgagcgac aagagaaaag gccaaccgcg catacattcc actcgaaaaa
     2881 attttaaccg gatgattaat gaaatctcca atggcggcaa attcggttat ggattcatac
     2941 cgggcatcga tctgctcact ggttaggcca agaatggagg cagccaaata cacattttct
     3001 ttcccggtaa attcaggatt aaaactcgat cccaattcca gcagcgccgc gactcggccc
     3061 ttgacagtca aatcgccaca ggtcggcgag agtgtgccgc acaccatttg taaaaacgtc
     3121 gacttcccgg atccatttcg accaataatc ccgaccgttt ctcctcgata cacgctcaga
     3181 tccacaccct ggaggcccca aaactcttca tagaactgtc ggtgccctct ccataacatc
     3241 tgcttcaacc gatcttcagg ctttcgaaag atctgatagg cctttccaag tccttgaacg
     3301 cgtatagcca gttcagacga catcagcaaa tccttttcgc gttttttgga accatgcata
     3361 acccaaccaa cccaccatcc aaaacacaac catatacagg aaccagtcta accattccgg
     3421 catttgtccc caaaatatga ccgatttcga cgcattcaga acaggtgtca atggattgag
     3481 ctgaatcaac cctcgatagg cttcaggaat agcagacagc ggataaaaaa taggactgag
     3541 aaacatcatc atcgtcgcca tcaccccgac caaaggttgg acatcacgaa gaaaaacccc
     3601 cactgaggcc agaaaccagc ccaaccctac aatcaacaac acaaatggaa agagtacgac
     3661 cggcaacagc aacacggtta gtggtggaag tccatggact acgaggtaaa aaaccaacaa
     3721 aatcaaaaga ctcacggcgg cgttaaacag tgccacctgc atagacaccc aaggcattat
     3781 ttccagagga aacactacct ttttaacata tgacacattt tcgagcataa gacccggagc
     3841 acgggtgaca cattcggaaa ataaattaaa aataatcaat ccagaaaata ataacagagc
     3901 aaaggcgcca ttttcctcca tgggcacctc ccatcgaatt cgaaacacca cagaaaaaac
     3961 aaacgtatac acccccagca tcaacatcgg gacaacaaag gcccagatga ccccaagcat
     4021 cgaccctcga taacgcgcat caatctctcg cttggcaaga cgagcaatca atgttctgtg
     4081 ctcccaggcg caaatcaatg gcgaaaaggg attatacgca ttattcattg gttaattatc
     4141 aatctcattc aagattatcg ttgaactaat ttcagtaaat tg
//
LOCUS       DAORAQ010000221         4113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3054_length_4113_cov_63.761952, whole genome shotgun sequence.
ACCESSION   DAORAQ010000221 DAORAQ010000000
VERSION     DAORAQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3054_length_4113_cov_63.761952"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKM72_13035"
     CDS             <1..662
                     /locus_tag="PKM72_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNP61762.1"
                     /translation="YAVVGVFESGMFEYDSSLSYISLEEAQKFFNLGETVTGIQVKVN
                     DVFLAQQIAQRLEATLGSTFIARDWMQLNRNLFSALKLEKTMMFLLLVLITLVASFNI
                     VGTLTMIVNEKQREIAILKAMGATPGAIMRIFMLNGLVIGIVGTGIGVPLGYTFLWLI
                     QNYWTFDASVYYISRIPVHILPLDVLLVATSAILISFAATFYPSWQAAKLDPVSALRY
                     E"
     gene            655..1404
                     /locus_tag="PKM72_13040"
     CDS             655..1404
                     /locus_tag="PKM72_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP61763.1"
                     /translation="MNNPIAPEFTPQVPLVSIDNLCRSFQMGKQTVHVLNGITMTLAR
                     KEMVSIVGASGAGKSTLLHIMGTLDRPTSGEVLFEGKNMFRVSETALAGFRNRSIGFV
                     FQFHHLLPEFTALENTYLPALIQKIPKQQAMDAAKSLLSEVGLSHRLHHKPGELSGGE
                     QQRVAVARALIRHPDLVLADEPTGNLDTHTGDELFALLRKLNHTHGTAFVIVTHNEKL
                     SLQTDRLIQLQDGQIIEDRCLTDGSTTAKMS"
     gene            complement(1575..2333)
                     /locus_tag="PKM72_13045"
     CDS             complement(1575..2333)
                     /locus_tag="PKM72_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNP61764.1"
                     /translation="MNRWVVISIVLLMGLSGCMVSKSKYEASVADMESAKAELEKSRM
                     HQEALEEQIRNLKGLNEKVSTDLETMTTEVQRIKEGRKNEHTLLETRERELDQEKEQL
                     TSKLRVVVDQYQKVKAQNKALKETVLRYQKELKDTRESSIGMAAGAMKPSENPDMAKS
                     GISPPGASPIVSSKSPSAKIVSSPTLPKMGGDLVNINKAPVSDLVLTLGLTREVAEEV
                     VSNRPYRLRGELVAKNIIPKATFDGIKDRITASP"
     gene            complement(2500..3300)
                     /locus_tag="PKM72_13050"
     CDS             complement(2500..3300)
                     /locus_tag="PKM72_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNP61765.1"
                     /translation="MSTISGGFFQFSPVFAEVQRNLETILNAIHTFEGQLLVFPELAL
                     SGYQFISKDEVRELSEDIPSGPSIKALEEAIRGRDLHVVVGLAERDHDRLFNSAVLIG
                     PKGYIGTYRKTHLFFEETLWFAPGDTGFLVWDIGRAKVGLLVCFDWFFPEAARSLAIK
                     GADILCHPSNLVLPYCPDAMVTRCLENRVFAITANRVGREQRGEKPALPFIGMSEIVA
                     PNGRILCRSSQKNEELGLYEMDVAEARDKRINTYNDLLKDRRPEWYVL"
     gene            complement(3370..3726)
                     /gene="smbP"
                     /locus_tag="PKM72_13055"
     CDS             complement(3370..3726)
                     /gene="smbP"
                     /locus_tag="PKM72_13055"
                     /inference="COORDINATES: protein motif:HMM:NF028095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNP61766.1"
                     /translation="MRKQVVKKAIGIIAILGILVAGAWGSVALAGANPHVAEAIAHAE
                     GAVKHGEQGHADALVEHAQEALTHAQGAQKDVKNPHLDEGVHELMEAVEHGKAGHADV
                     GTKHAKSAVMHLKEVK"
     gene            complement(3802..>4113)
                     /locus_tag="PKM72_13060"
     CDS             complement(3802..>4113)
                     /locus_tag="PKM72_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNP61767.1"
                     /translation="HIPSAINIPWNRFLQPDATVKPPEQVREIFEDHGLRDNQEIMTY
                     CLGGVRAAWVFCLLRYVGFPRVKVYPGSWWEWSRDFAAPAEKDVKLLFRVTNQDQMKS
                     S"
BASE COUNT         1017 a         1140 c          882 g         1074 t
ORIGIN      
        1 cctatgccgt tgtcggtgtg ttcgaatccg gcatgttcga atatgattcg tcgttatcgt
       61 atatcagcct ggaagaagcc cagaaatttt tcaatctcgg tgaaacggtg acagggattc
      121 aagtcaaagt caacgatgta tttttggcgc agcagattgc ccaacgcctg gaagccacac
      181 tcggttccac cttcattgcc cgtgattgga tgcaattaaa tcgcaatttg ttttcggcat
      241 tgaaactcga aaagaccatg atgtttcttc tgctcgtctt aatcaccctg gtcgcgtcgt
      301 tcaatatcgt ggggaccttg acgatgattg ttaatgagaa acaacgggaa attgccatcc
      361 tcaaagccat gggcgccacg ccaggagcca tcatgcggat tttcatgcta aacggcttgg
      421 tgatcggtat cgtgggaacg ggcattgggg tccctctcgg ctataccttt ttgtggctca
      481 ttcagaacta ttggaccttc gacgcctccg tctattacat atcccgcatt cccgtgcata
      541 tcctgccctt ggatgtgctg ttggtcgcca cttcggccat cctcatcagt tttgccgcca
      601 cgttctatcc ctcatggcaa gcagcgaagc tagatccagt cagcgcacta cgttatgaat
      661 aaccccatcg ccccagagtt cactccccaa gtgccattgg tctccattga caatctttgt
      721 cgctcatttc agatgggcaa gcaaacggtg catgtcctca acggcattac catgacctta
      781 gcgcgaaagg aaatggtgtc gattgtcgga gcatcaggag cagggaaaag tacgttgctc
      841 catattatgg ggacattgga tcgcccgacc agcggagagg tcctgtttga aggcaagaat
      901 atgttccggg tcagcgaaac cgcgttagcg ggattccgga atcgatccat cggatttgtc
      961 tttcaatttc atcaccttct gccggaattt accgcgttgg aaaacaccta ccttccagcg
     1021 ctcattcaaa aaattcccaa acagcaggcc atggacgcgg caaaatcgct cctgtctgaa
     1081 gtggggctct ctcaccgcct gcaccataaa ccaggagaat tatccggagg agagcagcaa
     1141 cgtgtcgccg tagccagagc cttgatacgg caccctgacc tggtgttggc ggatgaaccg
     1201 acaggcaatc tcgacacgca taccggtgac gaattatttg cacttttacg aaaactcaat
     1261 cacacgcatg gaacagcctt cgtgattgtg acccacaacg aaaaactctc ccttcaaact
     1321 gaccggctga ttcaactgca ggatggacag atcatcgaag atcgctgtct gacggatggc
     1381 tccaccactg cgaaaatgtc ataagaccag gcacataaaa atggggaact ccatccctgt
     1441 tgatgaagtt ccccttgttg aggaatgcta tactctccag tcggtatcta tttattccac
     1501 aatcccttcc cccttttagc ttgaaggcaa tcttgtgggg tttatggcct caccaaccac
     1561 gccaattgga cctattacgg agaagccgtg atacggtcct tgattccgtc aaacgtggcc
     1621 ttgggaataa tatttttcgc cacaagttcg ccacggagcc gataaggccg gtttgaaacc
     1681 acttcctcgg ctacttcacg ggttaaccct agcgtcaaca ccaggtcact cacgggagcc
     1741 ttattaatat tgactaaatc ccctcccatt tttggaagtg ttggagaaga cacaattttg
     1801 gccgagggtg acttggacga aacgattgga ctagcccctg gcggagaaat tccgctcttg
     1861 gccatatctg gattttccga tggcttcatc gcgccagcag ccatcccgat ggaagactca
     1921 cgggtatctt ttaattcctt ctgatagcgc aacacagtct ctttgagggc tttattttgc
     1981 gccttcacct tttgatattg gtccacaacc acccgtaatt ttgatgtgag ttgctctttc
     2041 tcctgatcta attcccgttc acgtgtttcc aataaagtgt gctcattctt tcgcccttct
     2101 tttatccgtt gcacctcggt ggtcatggtc tctaaatccg tagaaacttt ttcgttcaat
     2161 ccctttaaat tgcgtatttg ttcttcaagg gcttcctgat gcatccggct tttttccaac
     2221 tcggccttgg ccgactccat atccgccacc gatgcctcat atttcgactt cgaaaccata
     2281 cacccagaca atcccatcag aagcacaatc gaaatgacca cccacctgtt cataatatcc
     2341 tcctcatccc taccaataga acgattcatt cttgattaaa gctcttcctg accataatct
     2401 ggttcttggg tttacacccc tatttcagga tttatgtcat cctcgcggtt atgtcaagcc
     2461 agtccaccgc cctggcctct agattccgca acttgcggct tacaagacat accattcagg
     2521 acgtcggtct ttgagcaaat cattataggt attgattcgt ttgtcccgag cttcggccac
     2581 atccatctca tacagaccaa gctcctcgtt cttttgggaa gatcgacata aaattcttcc
     2641 attgggggcc acaatctcac tcatgccaat aaatggcaat gccggttttt ccccacgctg
     2701 ttccctgccg actcgattgg ctgttatggc aaacaccctg ttttctaaac accttgtcac
     2761 catggcatca gggcaatagg gcaacaccaa attgctgggg tggcaaagaa tatcggcgcc
     2821 cttgatggcc agacttcttg cggcttccgg gaaaaaccaa tcaaaacaga cgagaagacc
     2881 aaccttggcc cttccaatat cccagacaag aaatcccgta tctccaggag caaaccagag
     2941 agtttcctca aaaaacaaat gggttttccg ataggtcccg atatacccct tgggcccaat
     3001 cagtactgcc gaattaaata accggtcatg gtctcgttca gccaacccga ccaccacatg
     3061 cagatctcgt cctcgaatgg cctcttccaa ggctttgatg gagggaccag aaggaatgtc
     3121 ttctgacagc tcccggactt cgtccttgga aatgaattga tatcccgaga gggcaagttc
     3181 gggaaagaca agaagctgtc cctcaaaggt atggatggca ttcaggattg tctccaaatt
     3241 gcgttgcact tctgcaaaca caggagaaaa ttggaaaaac cctccactga tcgttgacat
     3301 caccacccca caaaaaaagc gggcagaggt ggactctacc cgctttttta tcagattaag
     3361 actttatgat tacttaacct ctttcaaatg catcactgca cttttcgcat gcttggtccc
     3421 gacatcggca tgaccggctt tgccatgctc caccgcttcc atgagttcgt gaacgccttc
     3481 atctaaatga ggattcttca cgtctttttg agcaccctgg gcgtgggtca aagcttcttg
     3541 agcatgctca accaacgcat cagcatgtcc ctgctcgcca tgtttcacgg caccctcggc
     3601 atgagcaatc gcttcagcta catgtggatt ggctcctgca agcgcgacag acccccacgc
     3661 ccctgcaacc agaatcccga gtatggcgat gatgccaatt gccttcttaa ccacttgctt
     3721 tctcatccct gacctccttg aaaaagtaaa tagaaattga actgagagat taagttatcc
     3781 tctcattcct catacacaag atcaagatga tttcatctga tcctgattgg tgacccgaaa
     3841 taataacttc acgtcttttt ccgccggtgc cgcgaaatcc cgggaccatt cccaccacga
     3901 tcctggatag actttcactc gtggaaaccc gacgtatcga agcaaacaaa acacccaagc
     3961 cgcccggacc ccacccaggc aatacgtcat gatttcctga ttatcccgta gcccgtgatc
     4021 ttcaaatatt tccctgacct gctcgggagg tttcaccgtg gcatccggtt gtaaaaaccg
     4081 attccacggg atattgatgg cagaaggaat atg
//
LOCUS       DAORAQ010000222         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3063_length_4101_cov_61.683638, whole genome shotgun sequence.
ACCESSION   DAORAQ010000222 DAORAQ010000000
VERSION     DAORAQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_4101_cov_61.683638"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..591
                     /locus_tag="PKM72_13065"
     CDS             364..591
                     /locus_tag="PKM72_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNP61768.1"
                     /translation="MNADQFKGKWMQFKGEFKQRYGKFTDDVLRQIKGIDDTIIGKVQ
                     ERYGDKKDALMKWEKQGQNSSTAETAHPNIP"
     gene            complement(716..916)
                     /locus_tag="PKM72_13070"
     CDS             complement(716..916)
                     /locus_tag="PKM72_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61769.1"
                     /translation="MNGPPPVVELHRWVTLLRRLVVNSIVIDRAFEPCLSISGLVLIR
                     PPDALPRVSNRGLLTRLAYDVR"
     gene            1269..1640
                     /locus_tag="PKM72_13075"
     CDS             1269..1640
                     /locus_tag="PKM72_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61770.1"
                     /translation="MIKQHILVIDDNAAIRNLLKIRLEYSGHTVDLAENGGEGLMMLA
                     QADYDLVLLDYMMPGITGLTVLEDIKERYPSLAVVMLTGHSGCHIEGQAIAAGARACL
                     YKPFNWVELEDILRCSVGTAS"
     gene            1704..2369
                     /locus_tag="PKM72_13080"
     CDS             1704..2369
                     /locus_tag="PKM72_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNP61771.1"
                     /translation="MAPKRRPSFNPKTFLRQVGNGKTIMSCGKNHVLFSQGDAADAVY
                     YILEGKVTLTVVSKRGKVAVVAILESATFFGEACLTGQVLRLEAATTVGVAKIVRIGK
                     AAMIQVLHEEPTFSELFLAHLLVRNLRIQEDLVDQLFNSSEKRLARLLLLMAHFGKES
                     EPETVIAKLSQETLADMIGTTRSRVSFFMNKFRKLGFIEYDSGSDKELHVHRSLLNVV
                     LHD"
     gene            2642..2857
                     /locus_tag="PKM72_13085"
     CDS             2642..2857
                     /locus_tag="PKM72_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61772.1"
                     /translation="MMNCVRCGGFQMLDQFYGMENNGSVWMYDAVRCLNCGSIEEPMR
                     EKVETISSPRAERLNQYALSVRAVDHQ"
     gene            3225..3854
                     /locus_tag="PKM72_13090"
     CDS             3225..3854
                     /locus_tag="PKM72_13090"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNP61773.1"
                     /translation="MNTYFRHLYRFTIGFWNPQPLLCLVPLSVTGEEPCDSRKHPSPC
                     HAIQCLCVVMLTGGFLTGCITDATTDLTKAPFDASTELTDASTDSFTRLTDGTSQATS
                     DLTEPTREFLSSTTPGAWFNADGMLNADHKKIAFVVVNFDNLQEDIARGSGEYLVSLS
                     MLMGVPPDSRDRFLQSAQRQHGYIFDESQSRPESFRRLIKTLHVTSPYS"
BASE COUNT         1034 a         1028 c         1041 g          998 t
ORIGIN      
        1 taagtacgtc caatggcaaa tgacagccta tcggtcccga gtccgggact ttacactcct
       61 tgtgggtgaa tgacctcacc attcaactcc aggccgtgtc tacagtagca gcccgcgtcc
      121 gtcacccctg cgattggcag ggttccagtc ggatgcagga cagtctgtgt ctgagcaggc
      181 cgttttttct gaatgctccc tccaaatccc ttccttaagg taaattttcc aacgtgagca
      241 atattgacca gacatcgtcc agctttcccc ccatagtgat ccatcattgg aagattagga
      301 atatcagggg ccgatcgagg cggtaccttg gtgtttcaat tccattaaga gagaggtaaa
      361 cgcatgaacg cagatcaatt caaaggaaaa tggatgcagt tcaaaggtga attcaagcag
      421 cggtacggca agttcaccga tgatgtttta cggcagatca aaggaattga cgacaccatt
      481 atcggcaagg tgcaggaacg gtacggcgac aaaaaagatg cactcatgaa gtgggagaaa
      541 caaggccaga actcgtctac ggccgagacg gcacacccaa atataccctg acgagaaatt
      601 tccaagaaga gtgcactgcg gaagcatcca atttagaaaa gaaaaaatca tgaaaatacc
      661 ctatctcatc atcgtgtgca ctgttttact tgccagcggg gccatcagcg tcggatcaac
      721 ggacatcata ggcaaggcgg gtgagaagac ctcgattact gacccgaggg agggcgtcgg
      781 gtggccgaat caagacgaga ccacttatag acaagcaggg ctcaaacgca cggtcaatca
      841 cgatggagtt aaccaccagg cgccggagaa gagtcaccca tcggtggagt tcgacgacag
      901 ggggtggtcc gttcatccca gtcggcttca tgccagccgt gtacaattgt caattttctc
      961 cttgtgtctt ctttcgatga agccattagg gcctgcgtaa cggagcggat ggccatgaat
     1021 gctcatatga ttccacagac caaagcataa aggacatcat gcacgagcga ggtccagatt
     1081 attctgatcc gttgctcaag gtagtgtgga cagcctttcg atttcaggag gtagtgttca
     1141 cggaaaagta tctcagaaaa acgagaatga ctcacgtatc gggaagcaca tgatctattt
     1201 cgaactaaaa ggggcgatac ctcgcaatcg catgccaatc catttctaat aggaaaggtc
     1261 ctcttgaaat gatcaagcaa cacattctcg tgatcgacga caacgctgcc attcgtaatc
     1321 tgctaaaaat ccgcctggaa tattccgggc acacggtaga tcttgctgaa aatggtggtg
     1381 aaggattgat gatgctcgca caggcggact atgacctcgt cctccttgat tatatgatgc
     1441 ctgggatcac gggtttgacg gtcttagagg acatcaagga gcggtatcct tccctagctg
     1501 tcgtgatgct caccggccat tctggctgcc acattgaggg ccaagcgatt gcggcagggg
     1561 cgcgggcctg tctgtataaa ccctttaatt gggtggaatt ggaagacatc cttcgttgtt
     1621 cggtcggcac ggcttcgtaa gaaggatggt ctccgagcgc ctgttacccg gagttaagcg
     1681 ccttttccat atcaaaagga tgtatggcac ctaaacggag accctcgttc aatccgaaaa
     1741 ccttcctgag acaagtcggg aatggcaaaa ccattatgtc gtgtggaaaa aaccatgttc
     1801 ttttttcgca aggagatgcg gcggatgccg tctattatat tctggaggga aaagtcacgc
     1861 tcacggtcgt ttccaaacgc ggcaaagtgg ccgttgtggc cattctagag tctgcgacct
     1921 tttttgggga agcctgtctc accgggcagg tattgcgttt ggaggccgcg acgacggtag
     1981 gtgtcgcaaa gatcgtacgc attgggaaag cggccatgat ccaggtgctt cacgaggaac
     2041 ccactttctc cgaactcttc ctggcccatc tcctggttcg aaacctccgc atccaggagg
     2101 acttggtcga ccaactcttc aattccagcg agaagcggtt ggcgcgcctg ttgctcctca
     2161 tggcccattt tggaaaagaa agcgaaccgg aaaccgtcat tgcgaaatta agtcaggaaa
     2221 cgcttgcgga tatgatcggc accacgcgct ctcgcgtgag tttctttatg aataagtttc
     2281 gcaaactggg cttcattgaa tacgacagcg gatcggacaa ggagttgcac gtgcacaggt
     2341 ctctcctgaa tgtcgtcctc cacgactaag tttcctcctt cggtttggtt tcccttccgt
     2401 tttcctttcc gttcagtcgg ttcaatgaag gtgagccgca acacctctag cggagcctcc
     2461 gtttccgttc tcacccctga gcaatattga ccagacaggc cttgcccggg ggcctataat
     2521 tttatggatg tgatgatatc gggtagtgat atcgggtagg ccgatcatag gccgagtttg
     2581 tacagaagag gcctgcagag agggccatct tgtgggaatg atcaaagata accaggaggt
     2641 tatgatgaat tgtgtacgtt gcggaggatt tcagatgttg gaccagttct acggcatgga
     2701 aaacaatgga tctgtttgga tgtatgatgc cgtgcggtgc ctcaattgtg gatccataga
     2761 agagcccatg agggaaaaag tggaaacgat ctcatcaccc agagcggaac gcctcaatca
     2821 atatgccttg agcgtgcggg cggtagacca ccaataggaa aaatcgggct cgttcctcat
     2881 cggcattctt gccatctatc tgaatcgggg agaccgtggc cgtatgaaat ccatgccgtc
     2941 tggtctattc aaaattccgg atttctcgca ataagctcac accgggagaa tgtcagtaag
     3001 gagccgtgaa cgtcccgacc gtccacctga acctgggtgg gtgtggtgag tcccgcccga
     3061 tccatcccgc atcaagacct gctacagaaa aaattaatgg gtgagtcaag aatacaaaaa
     3121 tccatccttc cagcatgaat ttggggaacg aacgggagca gggtggttgg atataatttc
     3181 tattcattca tccattcatt ttcctactca agggggaacc gcgcatgaat acctattttc
     3241 gccacctcta tcgtttcacc atagggtttt ggaatccaca gcccctcctc tgtcttgtgc
     3301 ccctttccgt tacaggagag gagccctgtg atagccgcaa gcatccgtct ccctgccatg
     3361 ccatacaatg tttgtgtgtc gtcatgctta caggaggatt tctcaccggg tgtattaccg
     3421 atgccactac tgacctgacc aaagctcctt ttgatgcttc caccgagctc acagatgctt
     3481 cgacagattc atttaccaga ctgaccgatg ggaccagtca agcgacgagc gatctcaccg
     3541 agcccaccag agaattcctc tccagcacga caccgggggc ctggtttaat gcagatggca
     3601 tgctcaatgc tgaccataaa aagattgcgt ttgtggtcgt gaactttgat aatttgcaag
     3661 aggatatcgc caggggatcc ggagaatatc tggtttcgct ttcgatgtta atgggtgtcc
     3721 ccccggattc ccgagaccgg tttttacaat ccgcacaacg gcaacatggc tatatttttg
     3781 atgagagcca atccaggcct gaatcattta gacgtttgat caaaacccta catgtgacgt
     3841 caccctactc gtaaagaacg gtctgatcag ccgaccttta gcctggggta tgcctcctgc
     3901 atgctgttga tcaaggccgg tatcgttttt gttccttctg cttctcgttt tcatttcgca
     3961 caaagggggc catactataa tttccgaagt tcattatcga gtagcagaaa aaacgaatag
     4021 attcccccta tccatccacc cttcaggtta gccgaatgac ctgacgtgcc aatcaatggg
     4081 ccgatcttcc cttacccctg c
//
LOCUS       DAORAQ010000223         4091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3079_length_4091_cov_57.454163, whole genome shotgun sequence.
ACCESSION   DAORAQ010000223 DAORAQ010000000
VERSION     DAORAQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_4091_cov_57.454163"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..781
                     /locus_tag="PKM72_13095"
     CDS             323..781
                     /locus_tag="PKM72_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61774.1"
                     /translation="MKNKMMLFAALIFMFGAVDYASGATTKPSFQFDPIEWPINTAEF
                     HLTQAQKLEQEATDLAAKVTELNQKIDKYEKKPYLDTKGFHRDGLKRIIGTTLQKVSA
                     LRGQVAWHRDEASRLATLDQVPQDNANDSEDRTTTLGDTSRSRSHSESPS"
     gene            933..1064
                     /locus_tag="PKM72_13100"
     CDS             933..1064
                     /locus_tag="PKM72_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61775.1"
                     /translation="MLGEAIVAMLTLMFLMWSAAIWASFKEDTEQKPGSLHSRKKVA"
     gene            complement(1218..1415)
                     /locus_tag="PKM72_13105"
     CDS             complement(1218..1415)
                     /locus_tag="PKM72_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61776.1"
                     /translation="MQKAFGQAGKGVFPGSTIMKAEICRQYQVEVVGDAGHVKQRFYA
                     QTVDRRIKHPSVLVITELAKQ"
     gene            complement(1959..2393)
                     /locus_tag="PKM72_13110"
     CDS             complement(1959..2393)
                     /locus_tag="PKM72_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61777.1"
                     /translation="MIQIQCQKKRRDWEWPKSLPHATIILATPLIYAMFIPLLVFDFC
                     VELYQRVVFPLLGLPLLSRREYIRLDRHRLPYLNPIQKAGCLYCGYANGLLQYASRIA
                     AETEKVFCPIRHQSGGKFHPPAHHIDFAPYGNAKEFQRKMGI"
     gene            complement(2516..3241)
                     /locus_tag="PKM72_13115"
     CDS             complement(2516..3241)
                     /locus_tag="PKM72_13115"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNP61778.1"
                     /translation="MCLIILGFLALTGFGTYYDKVPDEQLSSAIKERFRLDGRINADQ
                     IIIEVKRGHVMLSGVVDTVTEKILAEGLVAGSIIGVKSVVNNISVRPAVSQDDAIKQE
                     IDEYLKNTPALQGKTITVSVNHGIVKLEGLVETVFQRLAAEKAVELAKGVKGIVSLVK
                     INPSRPDREIEKEVALYLLWSPIVNIDGVDLSVHDGVVKLEGAVEHSAHILSLEQDIG
                     NIMGVVKVDVSKMTVKSRKSRAA"
     gene            complement(3385..>4091)
                     /locus_tag="PKM72_13120"
     CDS             complement(3385..>4091)
                     /locus_tag="PKM72_13120"
                     /inference="COORDINATES: protein motif:HMM:NF022391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HNP61779.1"
                     /translation="LEDGNTKDALAALAEVTGQLEIVISRDPDLAFVPVDVEVTTTDI
                     YADLDSIKKANEQAEDFLQNGEVQQARVLLSGLASEVVMTRVSLPLATYPDAIRAVAP
                     LIDQGEIEEAKAALQAVLENLVVTNERVVPLPILRAEAFIKTADDLAKQIETPALNKA
                     KSGDERDEAKNSENEKSQKEQVLEQLQNARRQLKMAEALGYGLEENRYKEFHESIEAI
                     EKKVQVRKAHKGYLPL"
BASE COUNT         1108 a         1013 c          854 g         1116 t
ORIGIN      
        1 tcgactgatc ttctcgtcta atgtaagccc ctactcgata tctttcagtc tcaccggata
       61 tttattctct ccgtttttcg aaattattat aaggttattc ttacagtgcc gtaggccgta
      121 aacctccatg attgggaaac atggattgaa attttcgatt ctgattataa gttttttcaa
      181 tttgcctctt tgcttttagg gcttatctta cttcacagaa ggcaccataa aagccctgtc
      241 gaaacaataa taaatgacac cacggaactg agaaaagtta ttgagcattc agatccttaa
      301 ctcaaaggaa ggaggtcata cgatgaagaa caagatgatg ttattcgcag cattgatctt
      361 catgtttgga gcggtggact atgcgtctgg tgccacaacg aagccctctt ttcagtttga
      421 ccccatagaa tggccaatca atacggctga gtttcaccta acccaggccc aaaaactcga
      481 acaagaggcc acagacttgg cggccaaggt gacggagttg aatcaaaaaa tcgacaagta
      541 cgagaagaag ccctacctgg ataccaaggg atttcaccgg gatggtctaa aaaggattat
      601 tgggacaact ttacaaaagg taagtgccct tcggggccaa gtcgcctggc atcgtgatga
      661 agcctcacgg ttggcgactc ttgaccaagt gcctcaagac aatgccaatg attcagaaga
      721 caggactact actttgggtg atacttctcg ctcacgctcg catagtgaga gtccatcatg
      781 aatataccgt gatgaagtgg attgtttgaa tgggttgtga gtaaaaaagc cttcgtgaca
      841 atgagcattg gaaatcgatt ggaaagactg gggaatacct attcaaaaac aggaggagtg
      901 ggaatcatca caaaatcgaa aggaggatga gcatgttagg agaagcaatc gtcgccatgc
      961 tgacgctcat gtttctgatg tggagtgccg ccatttgggc ttccttcaag gaggataccg
     1021 aacagaaacc cgggtccctc cattcaagga aaaaagtagc atagtcggat gagtgaggga
     1081 gccgggcctc atgttgtgaa cggagcattt ctcttgggac aagttggata acgtcaactc
     1141 caaaaaaaaa ggaaattatc acatgaccgg tccggctctt tcccatgatt cccttatttt
     1201 ccagccaatc caaatgttca ttgtttggcc aattctgtaa tcactagcac tgacgggtgc
     1261 ttaatccggc gatcgactgt ctgcgcatag aatcgttgct taacgtgacc cgcatctccc
     1321 accacctcaa cttgatattg gcgacaaatt tcagccttca tgattgtgga accagggaaa
     1381 actccctttc ctgcctgccc aaaggccttt tgcatggcac tatcttcaaa ctctcccacc
     1441 agttgcggat gaatatcatg aagagcaaat caattatcta aatcttggcg aaggactgta
     1501 ttggctttgg gtaataggaa cggtgctgaa tttagggact tccgataccc ctttcgataa
     1561 accttggcca actctgaagt cgcaaaaaaa catacgccgg attcaccaag aagatgatta
     1621 taggcttgaa ctcgaagaat cggaggaatc ggggtatcgg ccagaaccac atcaacggtt
     1681 agggatcact caattaagaa ttgttcaggc aatgtcacgg ctcttatcca gaattaatac
     1741 atatgctttt ccgtagccac atcggcctaa cggaatagat ccctccaatc ttcccaacca
     1801 attcactttg gtccaatatt tcctcataac ctaaataagc aagtataaac agtacaaaga
     1861 agtcgggaca aaatcaaaag gtaggctgac gaaggtgttt gcccaaatct gactcaatca
     1921 atcacatcga gttggaattc atgcgttgtg catgtaggtt agatacccat ttttctttgg
     1981 aattctttgg catttccata gggagcaaaa tcgatgtgat gagccggtgg atgaaatttg
     2041 ccgccgctct ggtgtcgaat gggacagaac accttttctg tttcggctgc aattcgagaa
     2101 gcatattgga gtaatccatt ggcatatcca caatacagac aaccggcttt ctgaatcgga
     2161 ttcagatagg gaagccgatg ccggtcaagt cggatatact ctcgacgcga taggagcggg
     2221 agtccgagta aagggaaaac cacccgctga tacaattcca cacagaaatc aaaaaccaat
     2281 agagggatga acatcgcata gatcaatggt gtggccagaa taattgtcgc atgaggtagg
     2341 gattttggcc attcccaatc tctacgtttc ttttggcatt ggatctgtat cacaaccttg
     2401 ttcattttat gataccccct ggcaacttcg gttggcctga cactgcccaa aagaacctat
     2461 tcaaatttct acaacttgca atctccaccg cctgaatccc atttgcccat cctcctcaag
     2521 ccgcacgaga tttcctgctt ttcactgtca ttttcgacac atcgactttc acaaccccca
     2581 taatatttcc aatgtcctgt tccaacgaca aaatatgcgc actatgctcg acagctcctt
     2641 ccaacttcac aacaccgtca tgtacggata aatccacacc atcgatattc acgataggcg
     2701 accacaataa atacaaggcg acttcttttt caatttcccg atctggacgt gaagggttaa
     2761 tcttcacaag gctgactatg ccctttacgc ccttggccaa ttcaactgcc ttttccgcgg
     2821 ctagccgctg aaacacggtt tccactaatc cttcaagctt aacgatgcca tggttcacac
     2881 tcacggtaat cgtttttccc tgaagggctg gcgtgttttt caaatactcg tctatttcct
     2941 gtttgattgc atcatcctgg ctcacggccg gtcgaacaga gatattattc acgaccgatt
     3001 tcaccccgat gatactccca gcaaccaggc cttcagcgag gattttttct gtcacggtat
     3061 ccactacccc acttagcatg acgtggcccc ttttcacttc aatgattatt tgatcggcgt
     3121 ttatccggcc atccaatctg aaacgctcct ttatggccga ggatagttgc tcatcaggaa
     3181 ctttatcgta atatgtcccg aagcccgtca gagcaaggaa ccccaggata attaaacaca
     3241 tccacgcgta tctttctctt tcacttacgg ttcgcattcg catttctcat ttcctctttc
     3301 caatcagcta ttgtcataaa tctgtgacgc gaggaaaaaa ttattcttcg aaaagaaatt
     3361 gcttaaactg agatactgac cccttcaaag aggcaaatat cccttgtggg ctttcctcac
     3421 ctgcaccttt ttctcaatcg cctcaatgga ctcgtgaaat tccttgtaac gattctcttc
     3481 taacccgtat cctaaggcct cagccatttt taactgacgt cgagcgttct gaagctgttc
     3541 cagaacttgt tctttttggg atttttcatt ttccgaattt tttgcctcat ccctctcatc
     3601 accacttttc gccttgttca acgctggagt ctctatttgt ttggctaaat cgtcagccgt
     3661 tttgatgaac gcttcagccc gaagaatggg cagtggaacg acgcgctcat tggtcacgac
     3721 taaattttct aataccgctt gaagagccgc cttcgcttcc tcaatttccc cttgatcaat
     3781 caatggcgct acagccctga tagcatcagg gtaggtagca agcggaagac tgaccctagt
     3841 catgaccacc tcacttgcaa gacctgagag gaggactctg gcttgctgca cttccccgtt
     3901 ctggagaaag tcttccgctt gttcattcgc ttttttaata ctgtcaagat cggcatagat
     3961 gtcagtcgta gtcacttcga catcgacggg aacaaacgcc aggtcgggat cccgcgaaat
     4021 aacaatttcc aattgtccgg tcacttcggc caatgcggcc agagcatctt tggtgttgcc
     4081 atcttccaaa g
//
LOCUS       DAORAQ010000224         4080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3092_length_4080_cov_101.001491, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000224 DAORAQ010000000
VERSION     DAORAQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3092_length_4080_cov_101.001491"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..200)
                     /locus_tag="PKM72_13125"
     CDS             complement(24..200)
                     /locus_tag="PKM72_13125"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNP61780.1"
                     /translation="MTSKKEMDRVCGMWIEPKEASFTSFYKDETYYFCAEECKVKFDL
                     NPAYYMVGVEGRKP"
     gene            complement(231..512)
                     /locus_tag="PKM72_13130"
     CDS             complement(231..512)
                     /locus_tag="PKM72_13130"
                     /inference="COORDINATES: protein motif:HMM:NF039498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HNP61781.1"
                     /translation="MRTLNLFVVTWSLATFCALSFTICVIYGLLTPHSLHMHEFLEMV
                     LPAYEWGSLPLFFVGLVESFLYGAYAGLVYVPVYNFFLKKCGPRCTLTQ"
     gene            complement(678..1655)
                     /locus_tag="PKM72_13135"
     CDS             complement(678..1655)
                     /locus_tag="PKM72_13135"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP61782.1"
                     /translation="MEPELLQPGDSASKPHRYDLIIIGGGPAGLTAAVYASLLRMRAF
                     LITTNIGGQTVEGSKIENYMGYEFISGEALIANFQDQLLQHHYLDHRIGEVTTVQRFG
                     DNFKVLTKEGEQYEASVLIIATGMRRRFLEVPGEAHLQRRGVFYQHLEEGALVTGLQV
                     AIVGGGNSALQGAIELSGLCPRVYVVSLGEWTADAAVQEDVKARGNVVALKGYAVTEI
                     YGDQRVTGVKVKCQKTGNERDLAVKGVFIEIGWRPFADVVSSLVDLNGQGEIQIRPDC
                     STSCPGIFAAGDVTDAFGKRVIIATGEGAKAALSAYEYLIKTGPRKVKG"
     gene            1884..2603
                     /locus_tag="PKM72_13140"
     CDS             1884..2603
                     /locus_tag="PKM72_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNP61783.1"
                     /translation="MQLQSPLFKAEGHTAANSHPIAFTVQELQAIFQSMKIQKEVSFL
                     NYYVFRHDTTPQPVFPSEIASLLAPHIATALSKAQPEETVVFVLSRPRKDGIPLITSG
                     GLFVRGEQFIIILAKVETPMTSKRKRAQVQANPLAPLNNPDFHFVVDPSQTIITPTDA
                     SELRPGSSAVPTVSIRYKALLAQSKDHALPLETVSDSPPSSPPAMVEEKLRQLKSWHQ
                     QGLIGEQEYRKKREEILASFQ"
     gene            complement(2667..3875)
                     /locus_tag="PKM72_13145"
     CDS             complement(2667..3875)
                     /locus_tag="PKM72_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61784.1"
                     /translation="MRKMLLTMMGVTFIASTITILILPNTASAVPSFSRLHGLPCHEC
                     HSAFPRLNPFGMDFKQRGFRLPREEGTYIWDQTTLPISALAVGRLRISHTDDPVTGAR
                     LSTKSAFEIEEVELMVAGTAAPKVGYLLEFAQEVADGEEFGTEQAWIQFSDLLPGSLL
                     NLRGGMMLNENYYLSQKRRLTFQRYLSPITFNVTGVELNGAWSGLRYAGGIVNDERES
                     GDYTPAVNLDKKLAGFYLWSTYTIADQVLGIRYINTKDNSDNPSPIIDGRTRQQLDVT
                     MNLWHGPLELILGYFQNWDIGGVNRQQRQNYLAEAIVEILPEKLFLDGRFELQNTAPV
                     GGSPNNPSTANGTQVSVNLTYYLIPNIRLIAEFDKVSGEGMDIYSFPSPNSSATDSEE
                     RYMLGFQIGL"
BASE COUNT         1020 a         1118 c          892 g         1050 t
ORIGIN      
        1 tcgctccctt ttgatccgta aagtcaaggc ttccttccct caacccctac catgtagtaa
       61 gcagggttaa ggtcaaattt gaccttacat tcttccgcgc agaagtaata tgtttcatcc
      121 ttgtaaaaac tggtaaacga ggcttctttg ggctcaatcc acattccaca cactcggtcc
      181 atttcttttt ttgatgtcat gggaatctcc ttttccgctc catatttggt ttattgagta
      241 agtgtgcaac gaggcccaca ttttttaagg aagaagttat aaacgggcac atacactaat
      301 cctgcatagg ctccatacaa aaagctttcc acaagaccca caaaaaatag aggcaaactc
      361 ccccactcat aagccggaag caccatttct aaaaattcat gcatgtgcag gctatgggga
      421 gtcagaagac catagatcac acagatggtg aacgaaaggg cgcagaaagt cgccaaagac
      481 cacgtcacga caaaaaggtt tagggttctc atttctggtg gctcccatca ggaggggttt
      541 ttttaccatg ccctccatga ctatgatgtg aaaaatgaca ccagaacatc agtaacgcca
      601 cgacaataaa aaaaacattt tcttgaagga attccatcat ttcatgttcc ctttgttata
      661 atctttttca aaatgattca tccttttacc ttcctaggtc cagtctttat caagtattca
      721 taagcgctta gcgcagcttt tgccccttcg ccggtggcga taatgacccg tttgccaaag
      781 gcatcggtca catcccccgc cgcaaagatt ccaggacaag aagtcgaaca atccgggcga
      841 atttggattt cgccttggcc gttcagatcg acaagcgagg agaccacgtc ggcaaatggt
      901 ctccaaccta tctcgataaa gactcccttt actgccaaat ctcgttcgtt tcccgtcttc
      961 tgacatttca cctttacccc cgtaactcgt tgatccccat aaatttctgt cacggcgtaa
     1021 cccttcagcg cgacaacatt tccacgtgct ttcacatcct cttgaacagc agcatcggcg
     1081 gtccattctc cgagtgaaac cacatacacc cttgggcata ggcctgacag ttcaatggca
     1141 ccttgaaggg ccgaattgcc gcctcccact atggcaacct gcaaaccagt cacgagcgca
     1201 ccttcttcca gatgttgata gaacacccca cggcgctgta agtgcgcttc tcccggcacc
     1261 tccaagaaac gacggcgcat gccggtagcg atgattaaga cggaggcctc atactgctcc
     1321 ccctctttgg ttagaacttt aaagttgtcc ccaaaccgtt gaacggtcgt cacctcccca
     1381 atccggtggt ccaggtaatg gtgttggagc aattgatctt ggaagttggc aatcagcgct
     1441 tcgccggaaa tgaattcgta ccccatgtag ttttcgatct tcgatccctc gacggtctgg
     1501 cccccaatgt tggtggtgat caagaaggct cgcatcctta gaagagaagc atacaccgct
     1561 gcggtgagac cggctgggcc acccccaatg atgatcaggt cgtaccgatg tggcttggac
     1621 gcactatctc caggctgtag gagttctggt tccactagag acccctgcct cttagacgag
     1681 agaacgttcc atcgtctcca tcgttgctct tgcctctctc tctacctata atttggctca
     1741 tgaaagttct cgtagtttct agccgtagaa gattgacgaa gcggcattcg agtttcgtag
     1801 tctttatcat aatatttctt ctaaccggct gtgccggcgc acccattttt tctcgaccgg
     1861 tctaccaaga ccaaaatctt atcgtgcagc ttcaaagtcc cttatttaag gcagagggac
     1921 acacggccgc caacagtcat cccattgcct tcacggtgca agaacttcag gcaatcttcc
     1981 agtccatgaa gatccaaaag gaagtgagtt ttcttaatta ctatgtcttt cgacacgaca
     2041 cgacccccca acccgtcttt ccctccgaga tcgccagcct tttggctccg catatagcaa
     2101 ccgccctgag caaagcccag cctgaggaaa ccgtcgtctt tgttctgagt cgacctcgaa
     2161 aagacggcat tccgctcatc acttctggcg gactgtttgt taggggggag caattcatta
     2221 ttatcctcgc gaaggttgaa acgcccatga catcgaaacg gaagcgagcg caagtccagg
     2281 caaatccact cgcaccgcta aataatcctg atttccactt tgtggtcgat ccctctcaga
     2341 cgattatcac gccaaccgac gcctcggaat tgcgcccagg aagctccgct gtacccacgg
     2401 tttcgatccg ttacaaggcc ttgttggccc aatccaaaga ccatgctctc cccctcgaaa
     2461 ccgtttctga ttccccgcct tcctctcccc cggccatggt tgaggagaag cttcgacaac
     2521 tcaagtcatg gcatcagcaa ggattaatcg gtgaacaaga ataccggaaa aagcgtgaag
     2581 agattctggc ctccttccag taaacccttt tccccaaaac aagggcatga gcgcccgcca
     2641 gcctaatcca taatcattga ggtctttcat agtccgatct gaaacccgag catgtaccgc
     2701 tcttcgctat ctgtagcact actatttgga ctaggaaaac tataaatatc cattccctcg
     2761 ccgcttactt tgtcaaattc cgcgatcaat cggatattcg gaatcaggta gtaggtgaga
     2821 ttgaccgaaa cctgtgttcc attggccgta gatggattat tggggctccc tccgacaggg
     2881 gcggtattct gtaattcaaa gcgtccatcc agaaataatt tttccgggag aatttctaca
     2941 atagcttccg ctaagtaatt ttgacgttgt tgccggttca ccccaccaat gtcccaattt
     3001 tggaagtacc caagaatgag ttctagaggg ccatgccaga gattcatggt gacatcaagt
     3061 tgctgccgtg ttcggccatc gataataggc gaagggttat cggaattgtc tttggtattg
     3121 atatatcgaa ttcccagaac ttgatctgcg atggtatagg ttgaccaaag gtagaatccc
     3181 gccaacttct tatccaaatt aacggctgga gtataatccc cggattcccg ctcgtcgttg
     3241 acgattcctc ccgcgtaccg cagtccactc caggctccat tcaactccac accggtgaca
     3301 ttaaaggtga tcggggaaag ataccgttga aaggtgagtc gccgtttctg ggaaagataa
     3361 taattttcat tcagcatcat tcctccacga agattgagga ggctcccagg taacagatcg
     3421 ctaaattgga tccaggcctg ttccgtgcca aattcctcac catcggccac ctcttgtgca
     3481 aactccaaca agtatcccac tttgggcgca gccgtaccgg caaccatcag ttcgacttct
     3541 tcgatctcaa acgcactttt ggtactcagg cgtgccccgg tgacggggtc gtccgtatgg
     3601 ctgatgcgca accgccctac ggccaaggct gaaatgggaa gcgtagtctg atcccaaatg
     3661 taggtgccct cttcgcgggg aagtcgaaac cctcgttgtt taaaatccat gccaaacggg
     3721 ttcaatcgag gaaatgccga atggcattca tgacatggca agccatgcaa acgggaaaaa
     3781 gatggaacgg ctgatgctgt gttgggaaga atcaaaattg tgattgtgct ggcaatgaag
     3841 gtgaccccca tcatggtgag caacattttt ctcatggcgc cctccttgaa aaataatgac
     3901 cctctttgat tttccgttac gctctggctg tctgggatgg ccagaccttt ttgatactac
     3961 gaccaggaaa ggtcttctct atctattggt cacttttttc ccatcaaggt ccgcatgtaa
     4021 gcgaggacct gccaacgctg ctcttccgta aggtgtttcg catgaccttg catcttggtc
//
LOCUS       DAORAQ010000225         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3114_length_4053_cov_58.691596, whole genome shotgun sequence.
ACCESSION   DAORAQ010000225 DAORAQ010000000
VERSION     DAORAQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3114_length_4053_cov_58.691596"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKM72_13150"
     CDS             <1..359
                     /locus_tag="PKM72_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNP61785.1"
                     /translation="VITEPVVCAHLLGQILLAFGEDHLLWGTDSIWYGTPQWQIEAFR
                     RFQIPEELQEKFHYPALTKTLKAKVFGLNAAKLFNVDVEAKRKDVPKDYLSHIKMAYL
                     DENPSPSHHAYGWVMA"
     gene            387..1547
                     /locus_tag="PKM72_13155"
     CDS             387..1547
                     /locus_tag="PKM72_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNP61786.1"
                     /translation="MSAKSGKVHTEEQLGQPHNESIEMEQHIGNSQTSEENKQQKLDR
                     ERTVLLSRLAEINFEDTRTKVAFILNQYRTTRDSDIALTLKYWQIFHRDLLGSNSISF
                     ENFHKLPKFTSISRARAKIQNEYGLFQASKDISKYRANLDSEQRDIQVEDKPEDSLIS
                     IYCDESGKTGRYKIVASLWINDGYRFMEVWNELSSWKADKGIEKELHFTKLGGQDIHN
                     VEEFFERVLCHSDALGFKAVILDSHPVQGISPEDLNFRLHYQLIVNGIEHEVQSGRFS
                     LPRRFSMTKDKDDGADKLRLVELEQKLRTDCQAYFNQNIIIEKVKAEESNSSLWIQLA
                     DLFAASINRKLNSPGSNYKDEIANKVLYLLGIRDIMDKTQDSHQDFATILWI"
     gene            1922..2365
                     /locus_tag="PKM72_13160"
     CDS             1922..2365
                     /locus_tag="PKM72_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61787.1"
                     /translation="MPTRRFSRFSILFLSLAGSLGLSLTAVIAHAQLETEPHPMDTFI
                     KGVTNFSEPTGVRGTVRYIEKDRETLWLNWKQRSDARPLFETGWKFVPGDATLAVQPR
                     DSEQWEKLLNLSQDLPLELIIQDDGKGHRHILSYEDISGPPKTPL"
     gene            complement(2425..2862)
                     /locus_tag="PKM72_13165"
     CDS             complement(2425..2862)
                     /locus_tag="PKM72_13165"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNP61788.1"
                     /translation="MTTAQYPRSPKEQVGGLCHLGRLIDKIRMRNAGQIQDYNYLTAG
                     LDKYLLDKLEIQGADIEKRVLQGGTDAEIADWVKSNGKSLTEEDKAEWNNMVLTFGPK
                     AEMAQKAFDRNKAALAEKRGVSVEELSHITTWTGLIDHDEDRM"
     gene            complement(3106..>4053)
                     /locus_tag="PKM72_13170"
     CDS             complement(3106..>4053)
                     /locus_tag="PKM72_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNP61789.1"
                     /translation="LSLLERDVTISWWGNVRFEPAFTPDLCRLLAASGCIALSAGLEA
                     ACDRLLALVKKGITVDQTVQVVQACHEAGILVHAYLMYGIPSETIGETIESLERVRQL
                     FAHDLIQSAFWHRFTTTAHSPIGLHPQTYGIQLVGPAFGGFAENDLVHVDALASIPEW
                     IGEGLRAALWHYKEGLELTRDVREWFPERVPKPKVKRTWVQQVLESEQREDHSRLERK
                     FVWIGGQPDVDRGNGEQYRIMLSNRTTDEEVRLKGGQGEWLLNLIERAVPVHQTGGHR
                     YPIYKEVRAGFPFGGPKGFDKWWQSASAIKARSVGLLLV"
BASE COUNT         1113 a          992 c          888 g         1060 t
ORIGIN      
        1 tcgtcatcac cgaacccgtc gtctgcgccc acctgctcgg acagattctt ttggctttcg
       61 gtgaagatca tcttctctgg ggaacagatt cgatttggta tggcacgcct caatggcaga
      121 tcgaagcctt ccggcgtttt cagattcccg aagaactcca ggaaaaattc cactatcctg
      181 ccctgaccaa aacactcaaa gccaaggttt ttggtctcaa tgcggccaaa ctattcaacg
      241 tagatgtcga agccaaacgg aaagatgtcc cgaaggatta tctcagccac atcaaaatgg
      301 cctatctgga tgaaaaccca tcccccagcc accacgccta tgggtgggta atggcctgat
      361 cactagagag tgacagattg tcctaaatgt cagcaaaatc aggaaaagtt cataccgagg
      421 aacaacttgg ccagcctcat aatgaatcga ttgagatgga acaacacatc ggaaatagcc
      481 aaacttccga agagaacaaa cagcaaaaac tcgatcgaga aagaacggtc ctactatcac
      541 gtctggctga gatcaacttt gaagacactc gcaccaaagt tgcgtttata ttaaatcaat
      601 atagaactac acgtgactcg gatatagcac tcacattgaa atactggcag atatttcatc
      661 gcgatttgct aggatctaat tccatttctt ttgaaaattt ccataaacta ccaaagttta
      721 catcaatatc tagggctaga gccaaaattc aaaatgagta tgggcttttc caggcgagta
      781 aggatatttc aaaatatcgg gcaaatcttg acagcgagca gcgagatatt caggtagagg
      841 ataaaccaga agattcttta atttctatct attgtgatga aagcggaaaa actggacgat
      901 ataaaatcgt tgccagttta tggattaatg atgggtatag atttatggag gtatggaacg
      961 aactttcgag ctggaaagcc gacaagggca tcgaaaagga acttcatttc acaaagttag
     1021 ggggacaaga catacataat gtcgaagaat tttttgaaag agttctctgc cattccgatg
     1081 ctcttggttt taaagcagta attttggatt ctcatccagt gcagggcata tccccagagg
     1141 atctcaattt tcgtttacat tatcagctaa tagttaacgg catcgaacat gaggttcaat
     1201 ctggccgttt tagtttaccc agacgttttt caatgaccaa agacaaggat gatggagcgg
     1261 ataaattgcg attagttgaa ttggagcaaa agctgagaac agactgtcaa gcctatttca
     1321 atcaaaatat aataattgaa aaagttaagg ctgaagaatc aaattcgagt ttatggattc
     1381 agttggctga tctctttgct gcaagcatta acaggaaact taactctcca ggttcgaact
     1441 acaaggatga aattgctaat aaggtattat atttattagg gataagggac ataatggata
     1501 aaacacaaga ttcccatcaa gattttgcga ctatcctttg gatataaatc ccttaatgtt
     1561 tcattgaaga tggagggaaa ttactttttt tgcaaattac attcggcctt tgtcttattc
     1621 gatgagttca aaccagactt aataatcaaa tcatggtcgg tcgacagttg ttgtcaaact
     1681 ccccattata gccatcctaa aaatctctga acttgttcag tcctggaatt gaaggtgagc
     1741 actatctgac tccatacccc ctgacatcat aaatccaatt ccatgaagtc ccaactttct
     1801 aaaaccccaa gttctagttg aatgttccaa ttcaaaagct cggcatttgt gaatcgtctc
     1861 tgcttttgat taaattaaac agtcgagaat atcattcttc ctcttacctg tgaggtgacc
     1921 catgccgaca cgacggttct cacggttttc aattctcttc ctcagcttag ccggatcctt
     1981 gggattgagc cttacggccg tcatcgctca tgctcagctc gaaactgaac ctcacccaat
     2041 ggataccttc ataaaagggg ttaccaattt ttctgaaccc acgggtgtcc ggggcaccgt
     2101 gcgttatatc gaaaaagacc gtgaaacact ctggttgaat tggaagcaac gatcggatgc
     2161 aagaccgttg ttcgaaaccg gatggaagtt cgttcccggg gatgccacct tagcggtcca
     2221 accacgggat tccgaacagt gggaaaaact gttaaacctt tcgcaagatc tgcctctgga
     2281 actcatcatc caggacgacg gaaaaggtca ccgccatatt ctctcctacg aggatatttc
     2341 cggtccgccg aaaactccgc tctaactcca aaccggtatt tctccagccg cacagatttt
     2401 tcctgccaga tattaagcca atggctacat ccggtcttcg tcgtggtcaa tgaggccggt
     2461 ccaggttgtg atgtgtgaga gttcctccac tgaaacccca cgcttctcag ccaaggccgc
     2521 tttatttcta tcaaatgctt tctgagccat ttcagccttt ggtccgaacg taaggaccat
     2581 gttgttccat tcggccttat cttcttcagt caggcttttc ccgttggatt tcacccagtc
     2641 ggcaatctcc gcatccgtac caccttgcaa cacgcgcttt tcaatatcgg ctccttgaat
     2701 ttccagttta tccagtaggt atttgtcgag tccggccgtc aaataattgt aatcttgaat
     2761 ttgtcccgca ttgcgcatac ggattttatc gatgagccgt ccgagatggc aaagcccacc
     2821 gacctgttct tttggactgc gtggatattg tgctgtggtc atcggtcata ctcctttgtg
     2881 ataaatgaat ctatgaaaac gtcaaatgtg gctcctacgt tatacctcgt gagaggcctg
     2941 aaaggaaatg ccggcccagg accaagctga gtcatatcaa cgggcttgca ctctctccag
     3001 aatcttctgg cagatggact ctggatagca gccggaaggt gaggacaaac ctaacgatgg
     3061 attccggtga atgatgtcgg aaatgataga ggagcttaat ccagcttaga ccaacaggag
     3121 accgaccgat ctggctttga tggctgaagc ggactgccac catttgtcga atcctttggg
     3181 gccgccaaac ggaaagcctg ctcgcacttc cttatagatg ggataccgat gtccccctgt
     3241 ttgatgaacc ggcacggcgc gttcgatgag attgagtaac cattcgcctt ggcctccctt
     3301 caacctcact tcttcatctg tggttcggtt cgacaacatg attctgtatt gctcgccatt
     3361 tccccgatca acgtcaggct gcccgccgat ccagacaaac tttcgctcta acctggaatg
     3421 gtcttcccgc tgttcgctct ccaggacctg ctggacccag gtgcgtttga cctttggttt
     3481 tggtacccgc tccggaaacc attccctgac atcccgggtc aattccaacc cttctttgta
     3541 atgccaaagc gccgctcgca acccctctcc gatccattcc ggtatagacg ccagagcatc
     3601 cacatgaacc agatcatttt ccgcaaaccc tccgaatgcc ggtccaacaa gctggattcc
     3661 ataggtttga ggatgtaagc cgatgggact gtgggcagta gtcgtaaaac gatgccaaaa
     3721 ggctgattgt atgagatcat gggcaaacaa ttgacgcaca cgttcgaggc tttcaatggt
     3781 ttcaccaatc gtctcactgg gaattccata catgagatag gcatgcacca aaatgcctgc
     3841 ttcatgacaa gcctgaacga cttgcacggt ttggtcgacg gtaatgcctt ttttgaccaa
     3901 ggccagcaga cgatcgcatg ccgcctccaa gccggcactc aacgcaatgc aaccggaggc
     3961 ggccagcaga cggcagaggt ccggcgtgaa tgccggttcg aaccggacgt ttccccacca
     4021 ggaaatagtg acgtcccgtt caagcagaga cag
//
LOCUS       DAORAQ010000226         4034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3125_length_4034_cov_71.613471, whole genome shotgun sequence.
ACCESSION   DAORAQ010000226 DAORAQ010000000
VERSION     DAORAQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_4034_cov_71.613471"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKM72_13175"
     CDS             complement(<1..860)
                     /locus_tag="PKM72_13175"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNP61790.1"
                     /translation="MPTTPTTAARPLLGTSLLQDGWITQEQLDLAVRETKRKGVMLGQ
                     TLIGLGFITEKILAHYLAEGTQTGVVDLSTIFIPPEIINLIPYDIATQFQVLPLSKQG
                     NVLNLAMADPLNIVAVDSIESRIGLNVHTHTAPAQELIEAIERHYAHRESIKQLLDEL
                     MAKGISDLGEDGSRVAPMIRLCNQLITAAIQSRVTDIHVEPDEYYLRVRMRIDGILTQ
                     EILIPKPLQAPITARFKLMANLDLTEKRTPQDGRIPFTLGTRRIDLRVSTLPTNFGES
                     IVLRILDKGA"
     gene            complement(991..1674)
                     /locus_tag="PKM72_13180"
     CDS             complement(991..1674)
                     /locus_tag="PKM72_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNP61791.1"
                     /translation="MPRKIFRPLHHSQGFTLVEMIGVLAIIAILIALLLPKIFTLIAS
                     SKASSLAAAVRTYETAVAKYYGDIGTIWPLNAAGTPAQETGGNSATVTSLAARLTLDA
                     SDPLNTGTNQWSRFRGPYLEKFNTNTPPGLGTTMYIPAQAAIALGTATTATNVGWDLK
                     GDDGNSDLPTGARVAYLRVDGVSDTEFNEIDGIIDAGIGSSLAERQLRGRVKYAAAND
                     RLYIYLTHQ"
     gene            complement(1813..2592)
                     /locus_tag="PKM72_13185"
     CDS             complement(1813..2592)
                     /locus_tag="PKM72_13185"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61792.1"
                     /translation="MRHPISKPRAKAKGTSRAPKASSVTFILSMIIGLGMLTWSSALG
                     NHLTLPGSQDLSHGDESDPQPFIRQLEKINNYIPPHAQADLENITSSRIEHSALPTNP
                     LHPSAIELKAVGSQQSHFTPTKKPIHTTVLFHNNATQDLSNSGWRSLLNGRPEDAITA
                     YRESLKTHPKSADAYIGMGIALKSLGFIEDAKDAIHHALAINPQLSSALVHLGYLYAD
                     GQIGPSDPKAAHRLFHQASQLGDPFASIALLDLQSRSRPQS"
     gene            complement(3196..3528)
                     /locus_tag="PKM72_13190"
     CDS             complement(3196..3528)
                     /locus_tag="PKM72_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNP61793.1"
                     /translation="MSTPIEDEINKEIAQHKILIYGKGTKTAPQCGFTVETMEFFNKF
                     GYPYELINALPNPEKREALSKMTNWPTLPKVFINGQFYGDTDVLGPMEEKGELQPLLK
                     AAFPDQAK"
     gene            complement(3533..3781)
                     /locus_tag="PKM72_13195"
     CDS             complement(3533..3781)
                     /locus_tag="PKM72_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNP61794.1"
                     /translation="MMTFEDLSTQLQQALGDAEVSVLDRTGTMDHFTVKVISNAFEGK
                     NLLDRHRMIYQALDAPMKDGRIHALEIQAKTRNEAQGG"
     gene            complement(3849..>4034)
                     /locus_tag="PKM72_13200"
     CDS             complement(3849..>4034)
                     /locus_tag="PKM72_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61795.1"
                     /translation="QGSLLPLLFSSILLLPPFLSLTFHALPQLGPGTLVAQNKQVKTS
                     EPHKEPFILGRPQNHIA"
BASE COUNT          940 a          866 c         1154 g         1074 t
ORIGIN      
        1 aatgcccctt tatccagaat tcggaggaca atgctttctc cgaaattcgt tggaagggtc
       61 gacacgcgaa gatcaatccg gcgggtgccc aaggtaaagg ggatacggcc gtcctgagga
      121 gttcgctttt ccgtcagatc gagattggcc ataagtttaa accgggcggt aataggcgct
      181 tgaaggggtt tcggaatgag aatttcttgt gtgagtatgc cgtcaatgcg catccgaacc
      241 cgtaaatagt actcgtccgg ctcgacatgg atgtcggtga cccgggattg aatggcggcg
      301 gtaatgagtt gattgcacaa gcgaatcatt ggggccaccc ggcttccatc ttctccaaga
      361 tcggagatgc ctttagccat taattcatca agcagttgct tgatggactc ccggtgcgca
      421 tagtgccgct cgatcgcttc gatcagttcc tgggcggggg cggtgtgagt atgtacattg
      481 agcccgatgc gactttcgat gctatccacc gcgacaatat tcaacggatc tgccatggcc
      541 aggttcaaca cattcccctg tttgctaaga gggaggactt gaaactgggt ggcgatatcg
      601 taggggatga gattgataat ttccggtggg atgaaaatag tggagaggtc gactacgccg
      661 gtttgggtgc cctcggccag ataatgggcc aggatttttt cagtgatgaa tcccaggcca
      721 atgagcgttt ggcccagcat tacgcctttt ctctttgtct ctcggaccgc caggtccaat
      781 tgctcttgag tgatccagcc gtcttgaagg agagacgttc cgaggagagg ccgtgcggct
      841 gtggtaggtg tggttggcat agaccaggct gtcaggagaa atttatgggc gcaaggaaat
      901 gggtgttgtc ctgtgaggga taccttggag ggaggagcac agccatcgtt gtaatttcct
      961 cactctttcc tcattgaggg aggacacgac ttattggtgc gtcaggtaaa tatacaggcg
     1021 atcattggcc gccgcatact tcacgcgtcc gcggagttgg cgttcggcca agcttgatcc
     1081 tatacccgca tcaatgatgc catcgatttc attgaattca gtatcagaca ctccatcgac
     1141 cctgagatag gcgactcggg ctccggtagg gagatcgctg tttccgtcat cgcccttcaa
     1201 gtcccatccc acatttgttg cggtggtcgc tgttccgagt gcgattgccg cttgtgctgg
     1261 aatgtacatg gttgttccta atcccggagg cgtattcgta ttaaactttt ccagataggg
     1321 tcctcggaat cggctccatt gattggtgcc ggtattcaat gggtccgagg cgtcgagcgt
     1381 taaccgggcg gcaagggaag tgacagtggc actgtttcct ccggtctctt gtgctggagt
     1441 tcctgccgca ttgagaggcc agatggttcc gatgtctcca taatatttgg cgacggcggt
     1501 ctcgtacgtt cttactgcgg cagccaggga acttgcctta gacgacgcaa tcagggtgaa
     1561 aatctttggc aataacaggg caatcagtat ggcaatgatg gccaggaccc cgatcatttc
     1621 caccaaggta aacccctggg aatgatgaag tggccgaaaa atttttcttg gcatctgtca
     1681 gttctccttt acgaatggtg cttcatttgg ccgtgtttca ggtgaaatag ggaaaatggt
     1741 atggatgaca cgtccataaa aaatccatcg gcatatgata tggccttctt aaggccaggc
     1801 gttccccgga aattaggatt gcggtcgtga gcgggattgc aggtctagaa gtgcgatgga
     1861 ggcaaatgga tcaccgagtt gggatgcttg atggaaaagc cggtgggcgg ccttgggatc
     1921 cgaaggtccg atctggccat ccgcatagag gtatcccaga tgaacaagtg cggaagagag
     1981 ttgagggttg atggcaagag cgtggtgtat ggcgtctttg gcatcttcaa taaagcccag
     2041 acttttaagt gcgatgccca ttccgatata ggcatcggct gatttgggat gggttttgag
     2101 ggattctcga tacgcagtta tcgcgtcttc cggccgacca ttaagcaatg accgccatcc
     2161 cgaatttgaa aggtcttgtg tggcattgtt gtgaaatagg actgttgtgt gaatgggttt
     2221 ctttgtggga gtgaaatggg attgttgaga tcccacagct ttgagttcga tggctgacgg
     2281 gtgaagggga ttggttggaa gagcggaatg ctcaattcgt gatgaggtga tgttttcgag
     2341 atccgcctga gcgtgggggg gaatatagtt gttaatcttt tctagttgcc ggatgaaagg
     2401 ttgagggtca gattcatctc catgactcaa gtcttgggag cccggtaggg tcaaatggtt
     2461 tcccaaggca gaagaccagg tcaacattcc cagaccaatg atcatactga gaataaaggt
     2521 gactgaggat gcttttggcg ctcttgatgt tcctttggct tttgcccttg gttttgagat
     2581 gggatgtctc atggttgctc attgcctcag tggttccaga ttgaacatat cggacaatag
     2641 gcaacatgct taaggaatta cgcgtgaagt gttgaaccaa ttcaaaaaaa cacagatcaa
     2701 aaggttcgca acggccaaca agagaacata tgtgcctgag agttgatgga gagcttagag
     2761 atgaatgaac ccatgcgatg gttcaacctc tgcgtcaggc agaagtaaca atgggtctgt
     2821 tgaaaaaagc cgccagcggc gttctcgtca ttttgccgtg ctcacgtact ggaggtacgc
     2881 tctgcgcgca aaaatgactg cggccttgct ggacgaaccc ttcgaaaggc tcagggcatg
     2941 cttttttgaa ccgcccccga aacctctaat atgcaacatg ccattggaaa ttattattca
     3001 acactccctc aatgtcctaa ggcataaatg cactgaggag tctgggctat gtatgtgaag
     3061 gtgcgtgatc gacaatggga aaagtctagg cttggtggca gagtgtgccg acgcaggtaa
     3121 ggtctaaaac aagcaacaag acccaccctt tattgaggtg ggccttgcag gaccgaaggc
     3181 cttttggctc aatgcctatt tggcttgatc gggaaaggcg gcctttaaca gtggctgcaa
     3241 ttcccctttt tcttccatcg gacccaagac atccgtatca ccataaaact ggccattgat
     3301 gaagactttt ggcaaagtcg gccagttggt catttttgaa agggcctccc gtttttcagg
     3361 gtttggcaag gcgttgatca attcataggg gtagccgaat ttgttaaaaa attccatggt
     3421 ctccaccgta aatccgcatt gaggggcagt cttcgtgcct ttcccgtaaa tgagaatttt
     3481 gtgttgggcg atttctttgt tgatttcgtc ttcaatgggt gtactcattg gcttatcctc
     3541 cttgggcttc attgcgggtt ttggcctgaa tttctagggc atgaatgcgt ccgtctttca
     3601 tgggcgcgtc gagggcttgg tagatcattc gatgacggtc taataaattc tttccttcaa
     3661 aggcattgga aatgactttg accgtaaaat ggtccatggt acctgttcga tcgagaaccg
     3721 agacttcggc atcgcctaag gcttgctgaa gttgagtgct gagatcctca aatgtcatca
     3781 tagaaaattt tgatcctggt gaaaaatgat ggaatattct gtccccacta taccggagaa
     3841 agaattttct acgcaatgtg gttctggggc cttcccagaa taaatggttc cttgtggggt
     3901 tccgatgttt tgacttgttt attctgggcg acgagtgttc ctggtccgag ttggggtaag
     3961 gcgtgaaacg tcagggataa aaatggcgga agtaagagga tagatgagaa gagaagcggg
     4021 aggagggaac cctg
//
LOCUS       DAORAQ010000227         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3149_length_4006_cov_58.828904, whole genome shotgun sequence.
ACCESSION   DAORAQ010000227 DAORAQ010000000
VERSION     DAORAQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3149_length_4006_cov_58.828904"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..886
                     /gene="cobS"
                     /locus_tag="PKM72_13205"
     CDS             110..886
                     /gene="cobS"
                     /locus_tag="PKM72_13205"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNP61796.1"
                     /translation="MRSLWASFSLAWQLLTIIPLPGGSGSKIPSVTFGASLRWFPLVG
                     FLLGASLVIIDRFLASLFPPVVLNMVMLTLYVLVTGGLHLDGWADTIDALSGGRDHEH
                     RLMILRDSRIGALGATGLILILGLRYAGLLSLPAGFREGMLFCMPAIGRWAMVIGCWG
                     VVYPRSEGLAAQFIRTVMRRDVLMATMVVGLGVWAMFDAVTAAMLLIIVCLVVRLVVW
                     WMSRKFGGITGDILGAMNEGVEVLFLILCPVLLVFSEFGK"
     gene            1133..2089
                     /gene="cbiB"
                     /locus_tag="PKM72_13210"
     CDS             1133..2089
                     /gene="cbiB"
                     /locus_tag="PKM72_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNP61797.1"
                     /translation="MADDLTGFKFLAVCVVDLMIGDPRWLPHPVRLMGVIIQRYERLT
                     LERISSTWAKKLAGMGLAVGLPLGAFFVTKGILVWAELVHEQFGMVIWVLLGSTTLAG
                     RDLWDHAMRVHRELKTGSLVSARVEVGRLVGRDTVDLSEEEIVRATVESVSENTSDGI
                     VAPLIYLALGGPACAMAYKAISTLDSMVGYRNDRYRDFGWASARADDLVNWVPARLTA
                     VAMSIAAAIRLGTGISAWKICWRDARDHPSPNSGWPEAAMAGALGVQLGGGNVYGGVR
                     EARARLGDRITTCSMALIPVALQVMGMAYGLLFLGIMGWVMW"
     gene            2083..3216
                     /gene="cobD"
                     /locus_tag="PKM72_13215"
     CDS             2083..3216
                     /gene="cobD"
                     /locus_tag="PKM72_13215"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNP61798.1"
                     /translation="MVTQPVVHGGRVHQVAKAFRRSVDSFMDFSASINPLGPPASVLR
                     AMQQAIPACGHYPDPSAEGLRKRLAKEHGISSDSIALGNGSSELIRNLPLALSLRHGY
                     VAAPTFMEYEESLYLAGARCTHVLANSGEQYAPPMEELSLLVDAICFSSQKAACKDGE
                     SHTAVFVCNPNSPTGRVVTARSLRTLYRKIEEAGLWMVVDEAFIDFCPSYSLIKEIST
                     ARRLLILRSFTKFYGMPGIRLGYLVGAPETVARIRRLLPPWSVSHFAQVAGVAAVGDV
                     RYRQRSVKFMRQERPRFIRQLRAVSGLRVIPTSANFVMVELPSTCVTAGLVARLARQG
                     ILVRDCQTFSGMTQPALRLAIRHPRDNNRVIHALKKALREGWD"
     gene            3426..3950
                     /locus_tag="PKM72_13220"
     CDS             3426..3950
                     /locus_tag="PKM72_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61799.1"
                     /translation="MSTQFKKIFGYLLVVFLVIGFFVLYRYVQPTGYQLPDDPLAMEA
                     LELVKKHRSREGYTLDEAVQLMVDDLKTKGIPFREGDWQVKAQGEDKYMVRKIVREKG
                     SVEWIEREYAWRVDSKEKSIRVISLAAQQLMPFENLPPLPHGDQISSLPHRMLKVTVL
                     TNVEGKSANGVLLT"
BASE COUNT          822 a          892 c         1210 g         1082 t
ORIGIN      
        1 cctgtttggc tatcggattg cttcaatcga gtttggcctg tttgacgcaa atggccacat
       61 tcgaaagcgc gggtgtggat gaggccgtac gaccgttccc ggaattggca tgaggagtct
      121 atgggcgtcg ttttctttgg cgtggcaact gctgacaatc attcctcttc caggaggttc
      181 ggggtcgaag attccttcag tgacgttcgg agcctcactt cgctggtttc ctcttgtcgg
      241 atttctgctt ggcgcgagtc ttgtcataat cgatcgattc ttggcgagcc tctttccgcc
      301 cgtcgtgttg aatatggtga tgctgactct ctacgtgctg gtcacaggag gtctccatct
      361 tgatggttgg gctgatacga ttgatgccct gtccggggga agggatcacg aacatcggct
      421 catgattctc cgggattctc gaatcggcgc gcttggggcc acaggcttga tattaattct
      481 tgggcttcgc tatgccggac ttctgtccct gccagcagga tttcgagagg gcatgttgtt
      541 ctgtatgccg gctattgggc gatgggcgat ggtgatcggt tgctggggtg ttgtctatcc
      601 ccgatcagaa gggttggctg cgcagtttat ccggacggtg atgcggcggg atgttctgat
      661 ggccacaatg gttgtggggt tgggggtgtg ggcaatgttt gatgcggtca ctgcggctat
      721 gctgctgatt attgtgtgcc tggtggttcg attggtcgtc tggtggatgt ccaggaagtt
      781 tggcgggatc acgggagata tattgggagc aatgaatgag ggggttgaag tgctctttct
      841 aattttatgt ccggtcctcc tcgtgttttc agagtttggg aaataatgaa caggacaatt
      901 cataaacggg aggccgattc cgtatgacac gaacctatcc taaacagtgt gaacgttgcg
      961 gtcgggcctt tgagtgcgga ctctcacgat gctggtgtaa ggatgttccc gtgagtgacg
     1021 cccaatatga cagcatcgcg aaacgttatg atgactgcgt ttgcccgact tgccttgcag
     1081 aattgaccgg aaacacgtct gacccttccg tatcaaaact cccgggctaa cggtggcgga
     1141 tgacctgacg gggttcaaat ttcttgcggt gtgcgtcgtt gatctgatga taggcgatcc
     1201 ccgctggttg cctcaccctg tccgtctgat gggggtcatc attcaaagat atgaacgcct
     1261 gacgttagag cgaatttcga gcacatgggc aaaaaaattg gccgggatgg gattggccgt
     1321 ggggcttccc cttggagcct tttttgtaac caagggtatc ctggtatggg ctgaactggt
     1381 gcatgaacaa tttggcatgg tgatatgggt gctgctgggc tctaccactc ttgccggtcg
     1441 ggatttgtgg gatcatgcca tgcgggtcca tcgggaattg aaaacggggt cactcgtatc
     1501 ggctcgggtt gaggtcggcc ggttggtcgg gcgggacacc gttgaccttt cagaagaaga
     1561 aattgttcgg gcgacagtag aatccgtatc tgaaaatacc tccgatggca tcgtggcgcc
     1621 gctgatctat ctcgcgttgg gcggacctgc ctgtgccatg gcctataaag ccatcagtac
     1681 gttggactcg atggttggct atcgtaatga tcgctatcgt gactttggct gggcatcggc
     1741 gcgagctgat gatcttgtta attgggttcc ggctcggctt acggcggtgg cgatgagtat
     1801 agccgcggcg attcgattgg gtaccggtat ttcagcctgg aaaatctgtt ggcgggatgc
     1861 tcgggatcac cccagtccga atagtgggtg gcccgaagcg gccatggctg gggctcttgg
     1921 agtccaactc ggtgggggta atgtgtatgg tggggtgcgg gaagcgaggg ccagattggg
     1981 tgatcggatc acgacctgtt caatggcact tattcctgtc gcgttacaag tcatggggat
     2041 ggcctatggg ctattgtttt tggggatcat gggttgggtg atgtggtgac gcagccggtt
     2101 gtgcatggag ggcgggttca tcaggtcgcg aaggcgttcc gacgatccgt ggattccttt
     2161 atggatttta gtgccagtat taatcccttg ggtccacccg cttccgtgtt gagggctatg
     2221 cagcaagcca tacccgcctg cgggcattat cccgatcctt ccgctgaagg gttgcgtaaa
     2281 cgactggcca aagagcatgg aatctcatcg gactcgattg cgttgggtaa cgggtcgtcc
     2341 gaattgattc ggaatttacc tctggcttta tcccttcggc atggatatgt ggcagctcct
     2401 acgtttatgg agtatgagga atctctttat ttagcaggtg cgcgttgtac gcatgtcctg
     2461 gccaactccg gcgaacagta tgctcctcct atggaagagc tttcgcttct cgtcgacgcg
     2521 atttgtttta gttctcagaa ggccgcgtgc aaggatggag aatcgcatac ggccgtgttt
     2581 gtttgtaatc cgaatagtcc gacgggaaga gtcgtgacgg cccgatccct tcgaacgctg
     2641 tatcggaaaa ttgaagaagc cgggctttgg atggtagttg acgaggcctt tatcgatttt
     2701 tgcccttcgt attccctcat caaggagatt tcgaccgcca gacgattgct cattctcaga
     2761 agctttacga aattttatgg aatgccggga attcgccttg ggtacctggt gggggcgccg
     2821 gagacggttg ccagaatacg tcggctactc cctccgtggt cggtcagcca ttttgcgcag
     2881 gtggcgggtg tcgcggcggt aggtgatgtc cggtatcggc aacggagtgt aaaatttatg
     2941 cgccaggaac gaccacgttt tataagacaa ttgcgggcag tctcggggtt gcgggtcatt
     3001 cccacctccg cgaattttgt gatggtggag ttaccttcga catgtgtgac cgccggcctt
     3061 gttgcacgac tggcccgaca agggattctg gttcgggatt gtcagacatt ctctggaatg
     3121 actcagccgg cacttcggct tgccatccgt cacccacgtg ataataatag ggtgattcat
     3181 gccctaaaaa aggccttgcg ggagggatgg gattaggaac ggtagacgga agatcaggct
     3241 gactactcct gattcccata atggcaagca acctcaggga ggatacctat agaagcacgc
     3301 aggaatcatg tgtgctataa catcaataga aatcgggtcc ccataggatc agatctctct
     3361 ggtcagtgga tgatcccctc taagtagata agaggttttt cagaaaataa caatggataa
     3421 atggcatgtc gactcaattt aaaaagatct tcggatatct tctcgttgtt ttcctggtta
     3481 taggattttt tgtgctgtat cgctatgtgc agccgacagg gtatcagctt ccagatgatc
     3541 ctcttgcgat ggaggccttg gagttggtca aaaaacaccg atcacgggag ggctatacgc
     3601 tggacgaagc ggttcaattg atggtggatg acttgaaaac caaaggtata ccttttcgtg
     3661 agggagattg gcaggtaaag gctcaaggtg aagataaata tatggtgcgt aagattgtgc
     3721 gggagaaagg ctccgtggag tggatcgagc gggaatatgc ctggagagtg gatagtaagg
     3781 aaaaatcgat ccgggtgatt tcgttagcag cccaacagtt gatgccgttt gagaatttgc
     3841 cgccacttcc ccatggagat caaatttcct cccttcctca tagaatgctg aaagttactg
     3901 tcctgaccaa tgtcgaagga aaatctgcta acggtgttct tcttacttaa acccctcacg
     3961 ttacccaccc gaacatttct ccttacccgt cttattcgtg aatggg
//
LOCUS       DAORAQ010000228         4004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3152_length_4004_cov_56.288427, whole genome shotgun sequence.
ACCESSION   DAORAQ010000228 DAORAQ010000000
VERSION     DAORAQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_4004_cov_56.288427"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PKM72_13225"
     CDS             complement(<1..827)
                     /locus_tag="PKM72_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNP61800.1"
                     /translation="MATELNMIQPSSSSSGDVCTYMWACAICDETETCQKDREGHSRW
                     LVGKRMERIDYKILVMSNKGGVGKSTMTTNLAISLALKGYEVGICDMDIHGPNIPKMV
                     GAEGQKLKISTGGGIIPHQAYNLKIASMSFLLQNSDDPIIWRDAYKFEFINQLLGGVE
                     WQDLNFLLIDLPPGTGNESVTTIDLIGDVTGCVIVSTPQEVALLDSRKSVTFARDSEI
                     PIIGIVENMSGLDCPHCHQSIELFRKGGGEASAGDMGVPFLGRVPLDPEMVLQCDRG"
     gene            complement(894..1373)
                     /locus_tag="PKM72_13230"
     CDS             complement(894..1373)
                     /locus_tag="PKM72_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNP61801.1"
                     /translation="MIRVAAILISSKIQSGQSHDENRKPLERILTNHQLTLLSYDVLA
                     DDRQAISRRLVTLCETTAPHIILTLGGTGVRPTDWAPEATRDVIEKEIPGIGEAMRAE
                     SLKKVRTAMLSRGTAGIRGTTLILNLPGSVKGMQENLDMVLPLLEHMVEKMGNVATQ"
     gene            complement(1398..2354)
                     /gene="tatC"
                     /locus_tag="PKM72_13235"
     CDS             complement(1398..2354)
                     /gene="tatC"
                     /locus_tag="PKM72_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNP61802.1"
                     /translation="MAKQSKMGSWFEEKVFKPLEDKKMPVMEHLHEFQWRLTRAVIVM
                     ACVFVGTFFYADTLVSWLRIPLQNYFILDSWEWMPSDLPKIPFVFLSPAEALWQNVKV
                     AGLCALVLSTPHWLWEVWQFVLPGLHAQERRFVGPFTAISTVAFYLGLTFCFFVVLPF
                     ALHFLISYGLASGFIAQISIANYVGFILWFMLVFGLIFEVPLALTLMAKLGWVDAPLL
                     RKYRKWAFLGSFLFAAILTPTPDPFNQCIMAIPMYFFYEVGIISAQVFGKKKGPEGED
                     ASAPAPATGPPAVRRPPLAPQPVGAGSGGDEEYVDVPDSGPR"
     gene            complement(2418..3968)
                     /locus_tag="PKM72_13240"
     CDS             complement(2418..3968)
                     /locus_tag="PKM72_13240"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNP61803.1"
                     /translation="MMHMSRWFAGAARPFRIAPPWGVLSLFLGLGMVGGFFGNGWLVP
                     AQTQAAIPGAFIEGFSEIVEKVGPAVVNVAVTGGGGGPSRGLPPGPFGGPPGGGPPGG
                     GPGGPPGGGPPGGGPGGPPGMSAGSGVIIDSRGYILTNNHVVEDASDIKVSFHDGREM
                     SATVVGTDPKTDLAVIKVKLENGPLPSVAWGEYESLRVGDVVLALGSPFGLRNTVSLG
                     IISALGRGSVGITEYEDFIQTDAAINPGNSGGALVNMKGELIGINTAIFSRTGGSEGV
                     GFAIAVSIAKDIAASLIETGKVVRGWMGVAIQELTPALAQSFQLPEGRQGGVLISEVH
                     EDGPSAKAGLQRGDVILEYGGEAVKDVNHLRNIVARTKVGKKKEIKVLREGKETILTL
                     ELGERPSDQALAKTGPVEEEKAPAMEKLDNVLSGMTVAPISGEHRSEFNIPEHIKGVV
                     VSKVESGSAVEAAGIQPGDVIQEVSRGSVKTIDDFKQIASKIAKDELAVLLVNRRGNN
                     LFIAVNPQ"
BASE COUNT         1044 a         1132 c          861 g          967 t
ORIGIN      
        1 tcaccacgat cacattgaag aaccatttca ggatccagtg gcacgcgacc gaggaagggg
       61 acacccatgt ccccggctga ggcctcgccg cctccttttc gaaacaactc aatcgattga
      121 tggcagtggg gacaatctaa gccgctcata ttttccacga tgccgatgat aggaatctcg
      181 ctgtctctgg cgaaagtgac cgattttcga gagtccaaga gagccacttc ttgcggagtg
      241 gacacaatca cgcagcctgt gacgtcacca atgaggtcaa tcgtcgtcac cgattcattg
      301 ccggtcccgg gcggaagatc gatgagcaga aaattgaggt cctgccattc gactccaccc
      361 aacagttgat taatgaattc aaacttatac gcatcccgcc aaatgatggg gtcatcggag
      421 ttttgcaaaa gaaaggacat cgaggcaatt ttcaaattgt aggcttgatg gggaataatc
      481 ccgccgcctg tactgatttt gagcttttga ccttcggctc ctaccatttt tggaatattg
      541 ggaccatgaa tatccatgtc gcaaatccct acttcatacc ctttaagtgc cagactaatg
      601 gccagattcg tcgtcatcgt acttttcccg accccgcctt tattactcat caccaaaatt
      661 ttgtaatcga tacgctccat ccgttttcca accaaccagc gactatgacc ttcacggtcc
      721 ttttggcagg tctcggtttc atcacagatt gcacaggccc acatataggt acagacatcc
      781 ccactcgaac ttgaggatgg ttgaatcata ttcagctcag ttgccatgaa tccctccaca
      841 tacattcaaa acgataattt tttggataat ataaattcgg ccttgcgcca atctcattgg
      901 gtggccacgt ttcccatttt ctccaccata tgctctaaaa gcggtaacac catatctaaa
      961 ttctcttgca taccttttac gctacccggc aaatttaaaa tcagggttgt ccccctaatt
     1021 cccgccgttc cgcgtgagag catggcagtc cgaacttttt taagactttc agccctcatg
     1081 gcttctccga ttccagggat ctctttttca atcacgtctc tagttgcctc tggagcccaa
     1141 tctgttggtc gcacccccgt tcccccaaga gtcaaaatga tatgaggagc cgttgtctca
     1201 caaagtgtga ctaatcgacg agaaatggcc tgacgatcgt cggctaggac atcataggat
     1261 aaaagggtca attggtggtt cgtcaagatc cgttccaatg gtttccgatt ttcatcgtgg
     1321 ctttggcctg actgtatttt gctactgatc aaaatggcag ctacccgtat catcagaagt
     1381 ccccaccggc gtaaatatta tcgaggacca gagtccggga catcgacgta ttcctcgtcc
     1441 cctccagatc cggctcccac aggttggggg gctaatgggg gacgccgaac ggccggtggg
     1501 ccggtggctg gagcgggtgc cgaagcatcc tcaccttccg ggcctttttt cttgccaaag
     1561 acttgggcac tgataatccc cacttcataa aagaaataca tcgggatggc cataatgcat
     1621 tggttaaatg gatcaggggt gggtgtcaga attgccgcaa agagaaagga gcccagaaat
     1681 gcccatttcc ggtattttcg taatagagga gcatccaccc accctaattt agccatcaag
     1741 gtcagagcga gcggaacttc aaaaatcagg ccaaaaacca acataaacca gagaataaaa
     1801 cccacataat tggcaataga tatttgtgca ataaatcccg aagccagccc ataggaaatc
     1861 aaaaagtgca acgcgaatgg caacaccaca aaaaagcaaa aggtcaatcc cagataaaag
     1921 gccaccgtgc taatggcagt gaaaggcccg acaaatcgac gttcctgggc atgcaagcca
     1981 ggaagaacaa attgccagac ttcccatagc cagtgcggcg tcgacaaaac cagtgcacat
     2041 aatccggcca ccttcacatt ttgccataag gcctcagccg gggagagaaa aacaaaagga
     2101 atttttggca aatccgatgg catccattcc caggaatcta aaatgaaata attttgcagg
     2161 ggaatccgta gccaggatac tagcgtatcc gcataaaaaa atgtcccaac aaagacacag
     2221 gccatgacaa tgactgcccg tgtgagccgc cattgaaact cgtgcaaatg ctccatgacg
     2281 ggcatttttt tgtcttccag tggcttgaag actttctctt caaaccagga acccattttg
     2341 ctttgtttgg ccatgtttgt tgtgtatccc aaaaatccga ggggggctca cgtcgccccc
     2401 ctccgaattt acatccctta ttgaggattc actgcgataa acagattatt gcctcgccgg
     2461 ttgaccagta gaacggccaa ctcatccttg gcaatttttg acgcaatctg cttgaagtcg
     2521 tcaatcgttt taacgcttcc gcgactcact tcttgaatga cgtcaccggg ttgaatccca
     2581 gcagcctcca cggcgctacc tgattcaacc ttcgaaacaa ccacaccttt aatgtgttcc
     2641 ggaatattga attcactccg gtgttcccca ctaatcggtg cgaccgtcat tcccgacaac
     2701 acattatcga gtttttccat ggccggagct ttttcttctt ctaccggacc agtctttgcc
     2761 aatgcttgat cggagggccg ctcgcccaac tccagggtca ggatcgtctc cttgccctct
     2821 cgtaacactt taatttcttt tttctttccc accttcgttc gagccacaat attacgaaga
     2881 tgattcacat ccttgacggc ttctcctccg tattcaagaa tgacatcgcc ccgctggagt
     2941 ccggcctttg ccgaagggcc atcttcatgc acttcgctga ttaacacccc gccctgtcga
     3001 ccttcaggta attggaaaga ttgcgctaag gcgggtgtta attcctgaat ggccactccc
     3061 atccatcctc ttaccacctt ccctgtttca atcagacttg cggcaatatc cttggcaata
     3121 ctaactgcga tggcaaatcc gactccttcg gacccgcctg tcctggagaa aatggccgta
     3181 ttgatcccga tgagctcccc tttcatattt accagggccc caccggaatt tccgggatta
     3241 atggcggcat ctgtctgaat gaaatcttca tattccgtaa taccgacact acctcgcccc
     3301 aaggcactga taattcccaa agacaccgta ttgcgtaatc caaatggact ccccagggcc
     3361 aggaccacat ctccaacacg aagggattca tattctcccc aggccactga aggaagaggg
     3421 ccattttcaa gtttgacttt aatgaccgct aaatcagtct ttggatcggt tcccacaact
     3481 gttgctgaca tctctcgtcc atcatgaaag gacactttga tatcgcttgc atcctccaca
     3541 acgtggttat tcgtcaaaat atatccgcga gaatcaataa tcactccaga ccccgcactc
     3601 attcctggag ggccgccggg accgccaccg ggagggccgc cgcctggagg gccaccggga
     3661 ccaccaccgg gagggccgcc gcctggaggg ccaccaaacg gcccgggagg taagcctctg
     3721 gatggtccac ctccaccacc agtcactgcc acattgacaa ctgcgggacc gaccttttca
     3781 acaatttctg aaaaaccttc aataaacgct ccgggtatag ccgcttgcgt ctgggcggga
     3841 accaaccacc catttccaaa aaatccacct accatcccca gtcctaaaaa taaagaaaga
     3901 accccccatg gaggggcaat tcgaaacggc cgggcagcgc cagcaaacca ccgtgacatg
     3961 tgcatcatcc cgatctcctg ttcaaaaaat ttaatagaaa cgga
//
LOCUS       DAORAQ010000229         3939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3221_length_3939_cov_73.362255, whole genome shotgun sequence.
ACCESSION   DAORAQ010000229 DAORAQ010000000
VERSION     DAORAQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3221_length_3939_cov_73.362255"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1430)
                     /locus_tag="PKM72_13245"
     CDS             complement(102..1430)
                     /locus_tag="PKM72_13245"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNP61804.1"
                     /translation="MKQKSHWLRRWGLAPTDRKEWALLFGLCGALGGCASLEFPDSAE
                     QESIEFNCAVGDVTPDEAIVWIKTSDAQTLTIQYSTDPQWSSFQETAMVSTTPETDFT
                     AQIPLGALKPKTRYWYRSLMQGKNPSQPCQFLTAPLAEDSTTVTFVISGDTRHSFQPF
                     SILESVRQVNPDFFVFLGDTIYADKDPPARELSAYWNKYRENRDGFAKRLFAETGVYV
                     MWDDHEVDNDFTSTHLLLPIGRQAFFDYWPIRRQRQDSTRLYRNFRWGKAVELFLLDT
                     RQYRNPATNSMLGEDQKQWLFAQLAASSAKFKFIISSVPFSDPRVDKWGEYPEERDEI
                     LEFLRKNGITGLLFLAGDVHYAAVSPMPGIINLKEYIFGPLAAPMHLDVSSEDPRFEY
                     LNNTSPNFGKITVQSDQFNPSVHIEWFDANRTLLHEVIIEDDRDHSLTSR"
     gene            1670..2236
                     /locus_tag="PKM72_13250"
     CDS             1670..2236
                     /locus_tag="PKM72_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61805.1"
                     /translation="MTPSPFLCIALLGAFHLTSQEEAVFAHQADHQPSYRVQSVAMKA
                     TPFSSIQPRTKLPIEPDEFDPREINAYYDWRNDMFFREFDLTGSGSVNFMTARRTYKV
                     WLDEFGTPVVLTVGDPVFFWIDLNGNGKFEQELGEMFKDSYEDGVTGNEEPYDNSDLQ
                     EPAGPGPQWNPPPQGGFKQPPCDPRFGC"
     gene            complement(2278..3087)
                     /locus_tag="PKM72_13255"
     CDS             complement(2278..3087)
                     /locus_tag="PKM72_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNP61806.1"
                     /translation="MKNNNVLWILLASLSLVMGLGASHAYEVGNVQAGGSIHGRVSFL
                     GSPPEPLHFKVEKNPEVCGQERSLMKVESRDGFLTGAVVVLEGVNMGKSYSPKTFTGN
                     LPGEGAFRYLGGESLGLQVKTKGCNFGPFTGVITANEAVRFQNQDSVKHVLHSFVSKD
                     TKGKILRTVHNQDLQPHDAIERIFPPDKMEDGVVVRIICNRHDFMQNWLYVVDNPYFA
                     ISDQEGRFTINEIPPGTYTLLVRHPVLGEQRQVVTIDAQANLQVVFQFADK"
     gene            complement(3148..>3939)
                     /locus_tag="PKM72_13260"
     CDS             complement(3148..>3939)
                     /locus_tag="PKM72_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61807.1"
                     /translation="KNMTTQMHMFSAMYGLNDTVTLMAMLPYLMKDMEHQNRAGANFS
                     TNSSGFGDTKLAALWRLYAFETPSIGSHRFHLNTGISLPTGDITPTDNTPLGNNSLLP
                     YPMRLGSGTVDLLPGLTYTGVRNDVSWGFQALGTVRIGHNNQGYKEGNRYKVNTWGAY
                     RWADWISTSARLNWQQWYNIKREDGQLQTTVGPAQIPLVPTADPDRQGGQRLDLMGGV
                     NILFPEFMGLENHLSVEAGAPIYQYLDGPQLRQAWSFWVGWQMVM"
BASE COUNT          963 a         1003 c          949 g         1024 t
ORIGIN      
        1 ggaacagatt tttttaacgg ccgcaatggc acctttccat attcccattt agaagggcag
       61 agatttaaag tcagtccaac agcttttatg gatttcctca tttaccggga ggtcaacgaa
      121 tggtcccgat catcctcaat gatgacttcg tgaagcaatg ttctgttcgc atcgaaccac
      181 tctatgtgga ccgacggatt gaattgatcg gattgaaccg taatttttcc gaaattgggt
      241 gaagtgttat tcaagtactc aaatcttggg tcctcactag aaacatccag gtgcatcggg
      301 gcagccagtg ggccgaaaat atattccttg agattaatta tcccaggcat cggacttacc
      361 gcagcataat gcacatctcc ggcaagaaat agcaggcccg tgatgccatt ttttcttaaa
      421 aattccagaa tctcatcacg ctcttcggga tattctcccc atttatcgac gcggggatct
      481 gaaaacggta ctgaagagat aataaactta aatttggcgg aagaagccgc gagctgtgca
      541 aacagccatt gtttttgatc ttctcccaac atggaattgg ttgccggatt tcgatattgg
      601 cgggtgtcca agagaaagag ttccacggcc tttccccagc gaaaatttcg atagaggcgc
      661 gttgagtctt gcctctgtcg acgaattggc caataatcaa aaaaggcctg gcgaccaata
      721 ggtaggagga gatgcgttga ggtgaagtca ttgtccacct cgtgatcgtc ccacatcaca
      781 tagacacccg tttcggcgaa aagccgtttg gcaaatccat cccggttttc tcgatactta
      841 ttccaatagg ctgataattc cctcgcgggc ggatctttat ctgcgtaaat ggtgtcgccc
      901 aaaaacacaa aaaaatcggg attcacctga cgcacgcttt ccagtatcga aaacggctga
      961 aaactatgtc gcgtatcgcc gctaatgaca aaagtaaccg ttgtggaatc ctctgcgagg
     1021 ggtgccgtaa ggaactggca tggctgactg gggtttttcc cttgcatcag gctccgatac
     1081 caataccggg tttttggttt cagagctcct aggggaatct gtgcggtgaa atcggtttcc
     1141 ggagtcgttg agaccattgc ggtttcttga aaggaagacc actgtgggtc ggtggaatat
     1201 tggattgtca gggtttgagc gtcggaggtt ttgatccaga cgatggcttc atctggtgtc
     1261 acatctccca cggcgcaatt gaattcgatg ctctcctgct ctgcactatc cgggaattct
     1321 agggaggcac accctcccag cgctccgcaa agcccgaaga gcaatgccca ttcttttctg
     1381 tcagtcgggg cgagtcccca acgccttagc cagtgggatt tctgcttcat tgttatggtg
     1441 aatcctttta aatttttcca gccggtggga atccccagtg agtcatagag ataggagtcg
     1501 gaaggggatg aagccgattt cgaataattt cctgaaacct tttagcgcgc gacaagcact
     1561 ccggataaaa tgagagagtg aaggtctctc gtcaaaattt gcgcctttgc gcttgcaacg
     1621 aatacaattt tgctagtctc cctgaaagac gaggaaaaga gcaacatcca tgacgccgtc
     1681 tccctttctc tgtattgcct tgcttggggc attccatctc acttcccagg aggaggctgt
     1741 ctttgctcat caagctgatc accaaccttc ttaccgggtt cagtctgtgg cgatgaaagc
     1801 caccccgttt tcttcgattc aaccacggac aaagcttccg attgagccgg atgaattcga
     1861 ccctcgggaa atcaatgcct actatgattg gcgcaacgat atgtttttca gggagttcga
     1921 cctgaccggt tctgggtccg tgaatttcat gacggcacgt cgaacctata aggtctggct
     1981 tgacgagttt ggcactccgg tcgttctgac ggttggcgac ccggtatttt tttggattga
     2041 tttaaacgga aacgggaagt ttgagcagga gttgggtgaa atgttcaaag attcctatga
     2101 ggatggggtt accgggaacg aagagcccta tgataatagc gatcttcaag agcctgccgg
     2161 tcctggcccc caatggaatc caccaccaca gggaggcttt aagcaaccgc cctgtgaccc
     2221 ccggttcggg tgttgaaggc tatcagatcc cgtgaccatc caggcttcag gtccctttca
     2281 tttatccgcg aactgaaata ccacctgcaa atttgcttgg gcgtctatgg tgaccacctg
     2341 gcgctgttca cccaacaccg gatgccggac caataaggtg tacgttcccg gaggaatttc
     2401 gttaattgtg aaccgaccct cctgatcgga aatggcgaaa tacggattgt ccactacata
     2461 cagccaattc tgcatgaagt catggcgatt acaaatgatc cgcaccacga caccgtcttc
     2521 cattttgtct gggggaaata tccgttcaat tgcatcatgg ggctggaggt cctgattatg
     2581 gaccgtacgc agaatttttc ccttggtatc cttggataca aaggagtgca ggacatgctt
     2641 gaccgaatcc tggttttgaa atcggacggc ttcgttggct gtgatcacac ccgtgaaggg
     2701 accaaaatta catcctttgg ttttgacttg aagcccgagg gactcccctc ccaggtatcg
     2761 aaatgcaccc tcgcccggta aattccccgt gaaggttttt ggggagtaag attttcccat
     2821 attgacgccc tccagcacga ccacagcacc agtcaagaat ccatctcgag attcaacttt
     2881 catgaggctt cgttcctggc cacaaacctc aggatttttt tctaccttaa agtgtaaagg
     2941 ctctggcggg gagcctagga aagaaaccct tccatggatg gatccaccag cctggacatt
     3001 ccccacttca taagcatggg acgcaccgag ccccatgacc agggatagac tagccagtaa
     3061 tatccacaac acattgttgt tcttcatgat ccatctcctt tttcctaggc tctccggaat
     3121 ctgagccatc cctatccaat cttttttcta catgaccatt tgccatccaa cccaaaaaga
     3181 ccaggcttgc ctcaattgcg gaccgtcgag atattgataa ataggcgcgc cggcttcaac
     3241 actcagatga ttctcgaggc ccataaactc cggaaacaga atattcacac ctcccataag
     3301 gtccaaccgc tgtccccctt gtcgatcagg atcggcggtt gggactagag ggatttgtgc
     3361 gggtccaact gtcgtttgga gttggccgtc ttctctcttg atgttgtacc attgttgcca
     3421 gttcaatcgc gcagacgtgc tgatccaatc agcccaacgg taggcccccc aggtgttcac
     3481 cttatatcga ttgccctcct tataaccctg attattgtgt ccaatacgaa cggttccaag
     3541 tgcctgaaat ccccacgaaa catcgttccg gacccccgta taggtcagcc cgggtaacag
     3601 atcgacggtt ccggaaccca agcgcatcgg atagggcagc aatgagttat tccctaacgg
     3661 tgtgttatcc gttggagtga tatctccggt cggcagagat atacctgtat tcaaatgaaa
     3721 ccgatgggaa ccgatactgg gcgtttcaaa ggcatataat cgccaaaggg ccgccaactt
     3781 ggtatccccg aatccactgg agttggtcga aaaatttgcc ccggctctgt tttgatgctc
     3841 catatctttc atgagatacg gaagcatggc catcagggta accgtatcat tcaagccata
     3901 catcgcactg aacatgtgca tctgcgtcgt catgttttt
//
LOCUS       DAORAQ010000230         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3242_length_3918_cov_71.160238, whole genome shotgun sequence.
ACCESSION   DAORAQ010000230 DAORAQ010000000
VERSION     DAORAQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_3918_cov_71.160238"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1180)
                     /locus_tag="PKM72_13265"
     CDS             complement(227..1180)
                     /locus_tag="PKM72_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNP61808.1"
                     /translation="MKRFKTILKPLIGILAIMPFSLIGLLVVGATGSKSGDKEPVTAQ
                     DTSVRVSYPTLHKTIEVDGLEIFYREAGPKDAPRILLLHGFPTSSHMFRNLIPQLSDR
                     FHVVAPDYPGFGNSSMPTVDEFSYTFDHLATVMEKFTRKVGLQSYSLYLMDYGAPVGF
                     RLAVSHPERVESLIIQNGNAYEEGLRDFWKPFRAYWKEHTPENAKALKGFLKLAATKW
                     QYTHGVRDPESISPDNWLVDQYLLDRTGNNDIQLQLFDSYGSNPPLYPKWQAYLREHQ
                     PPTLIVWGKNDHIFPAEGAYPYQRDLTNVEFYLLDTGHFAL"
     gene            complement(1356..1724)
                     /locus_tag="PKM72_13270"
     CDS             complement(1356..1724)
                     /locus_tag="PKM72_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNP61809.1"
                     /translation="MSTSTTIMLQDRLEDLFSYIREGRILDAITEFYAEDAVMQENAL
                     PPTVGRKANLKREKEFLSTVKEWKRFDVTAKSVGEDVTFYEAVMDWVATDGTPVHVEQ
                     VVVSKWQDGQIIHERYYHNP"
     gene            complement(1789..2337)
                     /locus_tag="PKM72_13275"
     CDS             complement(1789..2337)
                     /locus_tag="PKM72_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNP61810.1"
                     /translation="MSRLPAINPEHATGQAQRLLKGVESKLGFAPNIMRTMANSSSVL
                     QGYLDFSGALSKGSLSPKLREQIALAVSEANDCQYCLAAHSAIGRSVGLSEEALGDSR
                     RGESPDPKEATLLAFTRNVVTNRGWVSDEEVAKLRKAGFSEGDIVELIANISLTLFTN
                     YFNHIAATEIDFPAVSELEARV"
     gene            2507..>3918
                     /locus_tag="PKM72_13280"
     CDS             2507..>3918
                     /locus_tag="PKM72_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNP61811.1"
                     /translation="MRIHEISFTVSPMDSTITTTPECHEPTTLDSLKCLLLDIAQQRS
                     LNDLLWLIVRRLADRPTVVLARIWLIRPGDICSTCRLREECPDQTRCLHLVASAGRSG
                     VGDKTEWMNTDGYFARFPLGVCKVGRIGKTGEQLVINEMDNNLAWIARPDWARQEGIQ
                     GFHGQPIIYKGETLGVLAVFEREPVTNEATVWFRMIADHVAVAIANARAFEEIERLKA
                     QLELENTFLKEEVLEAQNFGEIVGRSPAIANLVQQIELVAPTDATALISGESGTGKEL
                     VAREIHRRSQRKDHPLVRVNCASIPRELYESEFFGHAKGAFTGALKDRAGRFQAADGG
                     TLFLDEVGEIPLEMQSKLLRVLQEGEYERVGEETTRKVNVRIIAATNRKLAQEVEAKR
                     FRQDLYYRLNVFPIEVAPLRNRKEDIPLLAARFMAHVRKKLNCTGRELTQAEVVKLQN
                     YHWPGNVRELQNIIERAVISS"
BASE COUNT          947 a         1040 c         1005 g          926 t
ORIGIN      
        1 gctgctgtga tcgcatccgt gttaacggtg ccatgatgat gcgattgggt aaggtaaggg
       61 aacctatggt taatggctca aatagttttg tcatgatctg tttccttgtg atgaaataaa
      121 tattacgtga cccgagcttt tcaatcatgg tgatcattca ccggtggtcc accattctgg
      181 tatcaaagaa acggcgtata tgaccggcaa tcacctctcc atcttcctaa agagcaaagt
      241 gtccggtgtc cagtaaataa aactccacat tcgtgagatc ccgttgatac ggatatgcgc
      301 cttcggcagg aaagatatga tcattcttgc cccacacgat taacgtcggt ggttgatgtt
      361 cacgcaggta cgcctgccat ttgggataca acggcggatt cgagccgtaa ctatcgaata
      421 gctgcaactg aatgtcgttg tttcccgtcc ggtccagcaa atactggtcc accaaccaat
      481 tgtccggact gatcgactcc ggatccctca cgccatgcgt gtactgccat ttggtggctg
      541 ccagcttgag aaatcccttg agagccttgg cgttctccgg cgtgtgctct ttccagtagg
      601 cccgaaaggg tttccaaaaa tcccgcaagc cctcttcgta cgcatttcca ttctggataa
      661 tgagcgactc aacccgttcg ggatgactga ccgccagccg gaagcctacc ggcgccccat
      721 aatccatgag atagaggctg tacgattgca agcccacttt ccgggtaaat ttctccatga
      781 cagtggcgag atggtcgaac gtgtagctga actcatccac cgtcggcatg gagctatttc
      841 caaatcctgg ataatccggt gccaccacat gaaaccgatc cgataattgt ggaatcagat
      901 tgcggaacat gtgagaggaa gtgggaaagc catggagcaa cagaatcctt ggcgcatctt
      961 tcggccctgc ttcccgataa aagatctcca gtccatccac ctcgatggtt ttgtggagag
     1021 tcggatagct gaccctcacg gaagtatctt gagcggtgac cggttccttg tctcccgact
     1081 tggagcccgt cgccccgacc accaacagac caatgaggct aaatggcatg atcgctaaaa
     1141 ttccaatcaa aggcttgagg attgttttga atcgtttcat gatggcggtt tccttttata
     1201 gggcgataac atttggagta ttcagaattg tggtttattc atcccccgaa tccacttttc
     1261 cctctcccta ttcaaaggga gacggaaagg gtaagggcgt gaactccggg actccctttc
     1321 ctcaccccaa atggccaaag aagtcctcaa aattttcacg ggttgtgata ataccgttcg
     1381 tggatgatct ggccgtcctg ccatttggag accaccacct gctccacatg gaccggcgtt
     1441 ccatccgtag ctacccaatc catgacggct tcataaaacg tcacatcttc gccgacgctt
     1501 ttggccgtca catcgaagcg cttccactct ttcaccgtgc tcagaaattc cttctcccgt
     1561 ttcaaattgg ctttgcggcc gactgtcggc gggagagcgt tctcctgcat aacggcatct
     1621 tccgcataga actcagtgat cgcatccaaa atgcggcctt cacgaatgta cgaaaacagg
     1681 tcttccagtc tgtcttgaag catgatcgtg gttgaggtgc tcatgggtat tctccttaat
     1741 taaaaaatga atgaatcatg atgaagtcaa atcagggttg ccggtgtttc acacgcgagc
     1801 ctcaagttcc gaaaccgccg ggaaatctat ttccgtcgcg gctatgtggt tgaaatagtt
     1861 cgtgaacagc gtcaaggata tattggcaat gagttccacg atatcccctt ctgagaatcc
     1921 ggctttgcgg agcttggcta cctcctcatc gctgacccac ccacggttcg tgaccacatt
     1981 ccgggtaaag gccaagaggg tcgcttcctt cgggtcgggg gactcccctc tccggctatc
     2041 cccgagagcc tcttcgctca gcccgaccga tcgtccaatg gccgaatgcg ctgccaggca
     2101 atactgacag tcattggctt ccgaaaccgc caaagcgatc tgctcacgaa gcttcgggga
     2161 aagactccct ttggaaaggg cgccagagaa atcgaggtag ccctgaagga ccgaagacga
     2221 attggccatc gtccgcataa tattgggggc aaacccaagt ttgctctcca cacctttcag
     2281 taatcgttgg gcctggccgg tggcgtgctc tgggttgatg gctggtaatc gtgacatcgg
     2341 gtaatccttt ctgttgattt tttaaaacca attttcattc cactgtttcg tggtattaca
     2401 atgaacatgc caaacaacga aaaatcgtaa gctgttgttt ttgtactaaa tttaatattt
     2461 aattcattcc tacttaccac gaattttcgt gtctaacgaa aaatcattgc gtatacacga
     2521 aatttcgttt accgtttcgc ctatggactc aacgattacg accacacccg agtgccacga
     2581 acccacaacc ctggattctc tcaaatgcct gttgctggac attgcccagc aacgttcatt
     2641 gaatgacctc ttgtggctga ttgtgcgacg gctggcggat cgacccacag tggtcctggc
     2701 ccgaatctgg ctgatccggc caggcgatat ctgctccacc tgccggttga gggaggaatg
     2761 cccggatcag acaaggtgcc tccatctggt ggccagtgcg ggtcgttcag gcgtggggga
     2821 taaaacggaa tggatgaaca ccgacggcta ctttgcccga tttccgcttg gcgtctgtaa
     2881 ggtagggcgg atcggaaaga ccggcgagca gctcgtcatc aatgaaatgg acaataatct
     2941 ggcctggatc gcgcggcccg actgggccag acaggaaggc atccagggct ttcacggtca
     3001 gcccatcatc tataagggag agactttggg cgtcctggcg gtgttcgaac gagaacccgt
     3061 caccaatgaa gccacggtct ggttccgcat gattgccgat catgtagccg tggcgatcgc
     3121 caatgcccgg gcctttgaag aaatcgaacg tctcaaagcc caactggaac tggaaaacac
     3181 cttcttaaaa gaagaagtcc tggaagcgca gaactttggc gaaattgtgg gtcggagtcc
     3241 ggccatcgcc aacctggtac agcagatcga actggtcgca cccaccgatg ccacggcgct
     3301 catttccgga gagtcgggaa caggaaaaga actggtcgcc cgcgaaatac atcgccgcag
     3361 tcaacggaaa gaccatccgc tcgtgcgcgt gaactgtgcg tccattccca gagaactcta
     3421 tgaaagtgaa tttttcgggc acgccaaagg cgccttcacc ggtgccttaa aagatcgggc
     3481 cgggcggttt caagcggccg atggcggcac gttgtttctg gatgaagtgg gagaaatccc
     3541 cttggagatg caaagcaaat tgttacgcgt cctacaggaa ggggaatatg aacgggtggg
     3601 agaggaaacc acgagaaagg tcaatgtccg catcatcgcg gccaccaacc ggaagctcgc
     3661 ccaggaggtt gaagctaagc ggttccggca ggatctctat taccgcctga atgtatttcc
     3721 catcgaagtg gcgccccttc gcaaccgcaa agaagacatc ccgctgctgg cggccaggtt
     3781 catggcccac gtccgcaaaa agctgaactg caccggaagg gaattgaccc aggcggaagt
     3841 ggtgaaattg caaaactacc attggccggg caacgtgcgg gaattacaaa atattattga
     3901 acgggccgtg atttcttc
//
LOCUS       DAORAQ010000231         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3250_length_3913_cov_68.653188, whole genome shotgun sequence.
ACCESSION   DAORAQ010000231 DAORAQ010000000
VERSION     DAORAQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3250_length_3913_cov_68.653188"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..819)
                     /locus_tag="PKM72_13285"
     CDS             complement(199..819)
                     /locus_tag="PKM72_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HNP61812.1"
                     /translation="MTTSYRVMPGPEHFLPPSAASMGIRLPDPGQGHIHGSIVSEEEA
                     LEEAARQFLSAKVPTIFPGPLVLWAWNDKAAKKAKAIRHLYETIKECVDSSCQQAMLI
                     PMPDYRPKYPKINPEIEINPNHPNLTIWHNKIDACMFVGVHCHQANLSLKIIRGGTNC
                     YTIAMCAQAGHEDAMLSVRDATADKIIRLADWVKKLKGSVPPPVKH"
     gene            complement(1187..1630)
                     /locus_tag="PKM72_13290"
     CDS             complement(1187..1630)
                     /locus_tag="PKM72_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61813.1"
                     /translation="MILVASYLPKFRRVLGVILMILFGAIAFIIWQDTQERELEFQRI
                     PISEVQLSHMDVRPGLNSRSFVLTGRLENAAQIFTIISVTIQASIEDCHATECEIVGQ
                     ETAEISLEIPPKQARDFSLTIPFSAIPKIIGEATWRYAILKVRAR"
     gene            1760..2584
                     /locus_tag="PKM72_13295"
     CDS             1760..2584
                     /locus_tag="PKM72_13295"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNP61814.1"
                     /translation="MPDFSHEIEIASHLARKAGEIIMEIYKNGFAVMYKGINDPVTKA
                     DQQANALIVDELHIHFPQDSIVAEESPLPCNSLTTGRTWYIDPLDGTKEFIAHNGEFS
                     IMIGLTIDAHAKLGVVYWPTRDELYAGLTDQTAWVERNGDRQKLFACESKCPTNISLV
                     TSRSHRSPALSTIKQSLPIHKEQQMGSVGLKIAQIAQGTADVYIEPSPYTKAWDACAP
                     EAILRGAGGYFTDIHGKPIQYGLNNFRNLHGLVGSTPDIHQRVIHALTGRCSSCES"
     gene            2665..>3913
                     /gene="trmFO"
                     /locus_tag="PKM72_13300"
     CDS             2665..>3913
                     /gene="trmFO"
                     /locus_tag="PKM72_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNP61815.1"
                     /translation="MRKDLVIIGGGLAGSEAAWQAANSGARVTLYEMRPKEETAAHKT
                     DHLAEIVCSNSLGSNDPLSAPGMLKEEMRQLNSLIIRIADEVRIPAGTALAVDRDQFA
                     SKVTQALAEHPNIKILREEIHEIPEDALCIIATGPLTSPKLSEAIIQFTQSKNLFFFD
                     AISPIVDADSLNTDRTFLASRYEKGTADYVNCPMDEETYNAFYNALIEADRVQPKSFE
                     NVPYFEGCLPIEVMADRGRQTLLFGPLKPVGLIDPKTGQRPYAVLQLRPENAYGSCYN
                     LVGFQTKLTYPEQRRIFRMIPGLEQAEFLRCGSIHRNTYINAPILLQNTLQVKKQPRI
                     FFAGQLVGVEGYVEAAASGGIAGINAARILSGRSPVTPPPSTAHGALLHYITTCEPKH
                     FQPINSNFGLYPPLDTPVRDKQKK"
BASE COUNT         1129 a          978 c          820 g          986 t
ORIGIN      
        1 gtatacacct gctctttttt gtttttttgt cctataaaag aagcttgagc ggccataact
       61 ttgacctcct ttttctgctt cagggtgaaa aacttcagat tttcctaaaa attatactca
      121 caatcacaga agaaatttta ttagaacaag cacttcatat tttcatctac aataaagaca
      181 gacccagaac gattccccct aatgtttcac cggaggagga accgacccct tcaacttctt
      241 tacccaatct gccaatctaa ttattttatc agctgtcgcg tcacgaacgg acaacatggc
      301 atcttcatgg cctgcttgcg cacacatagc aatagtataa cagttcgtgc caccacgaat
      361 gatcttgaga gaaagattgg cctgatggca atggacgccc acaaacatac aggcatcaat
      421 tttattatgc cagatggtca aatttggatg gttcgggtta atttcaattt caggattaat
      481 tttaggatac ttcggtctat aatccggcat gggaataagc atagcctgct gacacgaaga
      541 atccacacat tccttaatgg tttcatacaa atggcgaatt gctttggctt tcttggcagc
      601 cttgtcattc caagcccata ggaccaatgg tccagggaaa attgtaggaa ctttggccga
      661 aagaaactgt ctggctgctt cctccaaggc ttcttcttca gaaactatgc taccatgaat
      721 atgtccctga cctggatcgg ggagtcgaat ccccatagaa gccgccgacg gtggcaaaaa
      781 atgttcgggt cctggcataa ccctataaga agttgtcatc gtaccctcat gtctccatta
      841 aaaaaaaatt gacaatccta cccgttgcat ctccttactg taagaagaca ataagcaaat
      901 ctcaagatca aaaaagaccc accggtcaaa aatatttgac tttttgagaa tatgcctatg
      961 agtcttttgc cctataatta agaatattcc ttaataacct agcctagtat aagaaggcgc
     1021 attaaaaatt gtcaatttga caattgtcga taacagatgc aataggcaat ttttcacagt
     1081 aaagagcttc atgactgtca atttgttatt tgtttgaaga agtaaagacc taagattgag
     1141 aaaaaaggga atgaaaaccc ccggcaggca aaaaagagaa catggcctac ctggctctta
     1201 ctttcaaaat ggcgtatcgc cacgtagctt caccgataat ttttgggatc gcggaaaaag
     1261 gaatggttaa agaaaaatct ctcgcctgct tcggagggat ttccaacgag atctctgcgg
     1321 tttcttgccc aacaatttca cattccgtcg catggcaatc ctcaatacta gcctggatag
     1381 tcacggaaat gatggtaaag atttgagctg cattttcaag cctcccggtc agcacaaacg
     1441 atcgggagtt cagtccgggt cgaacgtcca tgtgagaaag ttggacctcg ctaataggaa
     1501 tccgctgaaa ctccaattcc cgctcctggg tatcctgcca aataatgaac gcgatggctc
     1561 caaacagaat catcaggatc acccctagga cgcgacgaaa tttcggcaaa tacgacgcga
     1621 ccaaaatcat gattaatatc aataggataa tagtaaatgg cagcatatat atcacaggcc
     1681 gtaggtgcga gaaaaattct cattcctctt ctccaccctt atcggccttc tgattcccct
     1741 tctttcaccg gaggaagtta tgccagattt ttctcatgaa atcgaaatcg cctcgcatct
     1801 ggcccggaag gctggggaga ttatcatgga aatctataaa aatggttttg cggtcatgta
     1861 caagggcatt aacgatcccg tcaccaaggc cgatcaacag gccaacgctc ttatcgtaga
     1921 tgaattacac atacatttcc cacaagattc aatcgtggcg gaggaaagcc ccctaccctg
     1981 caactcttta acgacaggcc ggacctggta catagatccc ctggatggaa cgaaagagtt
     2041 tattgcacac aacggggaat tttccatcat gatcggactg acgattgatg ctcatgccaa
     2101 acttggcgtc gtctactggc ctacccgtga cgaactctat gccggtctga cggaccagac
     2161 ggcttgggta gaacgcaacg gggaccgcca aaaactcttc gcttgcgaat ccaaatgccc
     2221 caccaacatt tcattagtta cctctcgctc ccatagatcc ccagcacttt ctaccattaa
     2281 acaatcatta cccatccaca aagaacaaca gatgggaagt gtgggactga aaattgctca
     2341 gattgcccaa ggaacagctg atgtctacat tgaacccagt ccgtatacca aagcatggga
     2401 tgcctgtgca cctgaagcca ttttgcgagg tgccggtggg tactttaccg atattcacgg
     2461 gaaacccatc caatatggcc tcaataattt tcgaaatctc cacgggttgg tcggaagcac
     2521 tccagacatt catcaacgcg tgattcatgc cttgacgggt cgatgttcct cgtgtgaaag
     2581 ttgacacctt tttccggcga ttcttagaat ttattcttta ataccttatt cagcgacttt
     2641 ttcaattatt attctcacac aattatgaga aaagacctcg tcattatcgg aggcggctta
     2701 gccggatcag aagcggcttg gcaggcggcc aactctggag ccagagtgac cctgtatgaa
     2761 atgcggccca aggaagagac agccgcgcat aagaccgatc acctagccga gatcgtctgc
     2821 tcaaattccc tgggatcgaa tgatcctctc agcgctccgg gtatgttgaa agaggagatg
     2881 cgccaattga actctttgat catccgaatt gccgacgagg tgcggatccc ggcagggacg
     2941 gcattagcag tagatagaga ccaatttgcc tccaaggtga cccaggcatt agccgaacac
     3001 ccgaatatca aaatacttcg agaagagatc catgaaattc ccgaggatgc gctgtgcata
     3061 attgccaccg gccccttgac ctcaccaaaa ttatctgagg cgatcatcca gttcactcaa
     3121 tcgaaaaacc tgtttttctt cgacgccatc tcaccaatcg tggatgccga ttccctcaac
     3181 acagaccgga cgtttctggc atcacgatat gaaaaaggta cggcggatta cgtgaattgt
     3241 cctatggacg aagaaaccta taacgccttt tataatgcat tgatcgaagc tgatcgcgtc
     3301 caaccaaagt ctttcgagaa cgtgccgtat tttgaagggt gtctcccaat tgaggtcatg
     3361 gccgaccgtg gtcggcaaac cttgttgttt ggtcccctca aacctgtggg attaattgat
     3421 cctaaaaccg gccaacgacc ctatgctgtg ctacaattac gtccagaaaa cgcatatggc
     3481 tcctgctaca atctggtagg atttcagaca aaactcacct acccggagca acgccgaatc
     3541 ttcagaatga tcccgggctt ggaacaggca gaatttttac gatgtggaag catccaccga
     3601 aatacgtata tcaatgcacc catcctctta caaaacacct tgcaagtaaa aaaacagcct
     3661 cgcatctttt ttgccggaca attggttgga gtagaaggtt atgtagaggc tgcagctagt
     3721 ggagggatag ccggaatcaa tgccgctcgc atcttatcag gccgttctcc agtcaccccc
     3781 ccaccctcta ccgctcatgg agcacttctc cattacatta cgacctgtga gccaaagcac
     3841 tttcaaccga ttaattccaa ctttgggcta tacccgccac ttgatacacc ggtgcgagac
     3901 aaacagaaaa aac
//
LOCUS       DAORAQ010000232         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3263_length_3901_cov_68.480499, whole genome shotgun sequence.
ACCESSION   DAORAQ010000232 DAORAQ010000000
VERSION     DAORAQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_3901_cov_68.480499"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..686
                     /locus_tag="PKM72_13305"
     CDS             153..686
                     /locus_tag="PKM72_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNP61816.1"
                     /translation="MVCLSSLLLGVRFLPGIIMVYLQAFLLIMAANAAPIFGRLLMKG
                     RWIAPLDGGATFFDGQPLLGPSKTYRGVAFSLVGTVLAADVLAMPWEIGLLTGGLAMT
                     GDCFSSFIKRRLGYASGGMALGLDQIPESLFPLLGLWGPLSLSPIGIMATAGAFMVFE
                     LFISPILYRFRIRKNPH"
     gene            complement(709..1026)
                     /locus_tag="PKM72_13310"
     CDS             complement(709..1026)
                     /locus_tag="PKM72_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61817.1"
                     /translation="MTFEELRTRWPWRPIRNCPGRLVLPRLEPPVSFEMLLGMPCSPQ
                     AFSSHQTKDRVLVWPLQDGGLITYEQPDGRLIHTLNTPAGFTRKIDQLGITLPKTKNL
                     SKE"
     gene            1161..1601
                     /locus_tag="PKM72_13315"
     CDS             1161..1601
                     /locus_tag="PKM72_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61818.1"
                     /translation="MNVDPSSEEESSTNSRAPLMSIGLAVFAVLMVPVILFSMGPDGP
                     AKVGDVVFVTDRHRVRLMTSDGDAIGPNPETCVLEPRVQLVVQKMGIPSGGSMIAEPV
                     VTEKVGRPFCFPGRPIVVYAHQVTLRPDLWGGLRDSLSHFFSGR"
     gene            complement(1637..2203)
                     /gene="msrA"
                     /locus_tag="PKM72_13320"
     CDS             complement(1637..2203)
                     /gene="msrA"
                     /locus_tag="PKM72_13320"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNP61819.1"
                     /translation="MSDSAIASSTGKELATLGGGCFWCLEAVFDQVKGVESVESGYMG
                     GSRPNPTYEAVCSGNTGHAEVIQITFNPGIITYRELLEIFFGIHDPTTLNRQGNDAGT
                     QYRSAIFTHSSAQEATAHEVIRTLTEAKLFDDPIVTQVEPASPFYMAEAYHQEYFVQN
                     PSQPYCAYLINPKVAKFRQRFSDKLKPS"
     gene            2429..3325
                     /locus_tag="PKM72_13325"
     CDS             2429..3325
                     /locus_tag="PKM72_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNP61820.1"
                     /translation="MRVVWATDIHLNFLGVEGRDAFFLSIRAQQPDVVFVTGDIAEAP
                     SLTHLIHEMRRAIHVPMYFVLGNHDFYYGSISQIRNDLKCWCQSQPGLIYLSTSGLVE
                     LTPTTALVGHDGWGDGRYGNYHLSPVRLSDQELIADFQDLDREAVLRKLRALGDEAAC
                     YLRDRLDEALSSYQRVICLTHVPPFKEACWYQGKMGNDDWLPYFACQAVGEVLLNVSR
                     ERPDCQITVLCGHTHHAGTVHLRPNLRVLTGSAEYGAPCIQESFDLEKLFPQSMLVGG
                     GEGEGGQSIEPDAAGRPSGPIK"
     gene            complement(3318..>3901)
                     /locus_tag="PKM72_13330"
     CDS             complement(3318..>3901)
                     /locus_tag="PKM72_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNP61821.1"
                     /translation="FSNVDLPWLVRRYRLRKPVWLSCIIMEGKLLIAERPKQIKDRSE
                     KRACYVKQGSSLPIHDWGKHEKVIVFDGVCNFCNAFVNFVLERDSRGLFKFGTLQSQP
                     AQDILTHLHLSTQDYETFLLLEHGKVFTKSTAALKILRCLPGAWPWLYGFIIIPRPLR
                     DMVYSFIARHRYQWMGRSNSCRVPTPNERTRFI"
BASE COUNT          814 a          906 c         1118 g         1063 t
ORIGIN      
        1 gacagtgtgt ttgcggcctt gtccaaggtc atacccagtc atctcctcga tcgtcgatta
       61 tttgatttcg aggcttttcg cgagcccgaa ccgtagatgg gcctgacggt ggttcccgtg
      121 cgaatgatga ccgacctgcc agttcctgtt ccatggtgtg tctttcctcg cttctcctgg
      181 gtgtgcggtt tttgccgggc atcattatgg tctatctcca ggcttttttg ttaattatgg
      241 cggccaatgc cgccccgatt ttcggtcggc tgcttatgaa agggcgttgg attgctcctc
      301 ttgatggggg agcgaccttc ttcgatgggc agccgttgtt gggaccctcg aaaacctatc
      361 gaggtgtggc tttctctttg gtgggtacgg ttctggcggc ggatgtgctg gctatgccat
      421 gggagatagg cctcctgacg ggggggttag cgatgaccgg cgattgcttc tccagcttta
      481 tcaagcgacg attgggctat gcctcaggcg ggatggcctt gggcttagac caaattccgg
      541 agagcctatt tcccctgtta gggctatggg gacccctatc gttatcgcct attggcataa
      601 tggccaccgc aggtgcgttt atggtgttcg aattgtttat ttctccgatt ttatacagat
      661 tccggattcg taaaaatccg cattaaattt gtgttcccgt tgccgagttt attccttgga
      721 gagatttttg gtcttgggga gggtaatccc caattggtca attttccggg taaacccggc
      781 cggcgtgttg agggtatgga ttaagcgccc atccggctgt tcgtaggtaa taaggccacc
      841 atcttgtagc ggccagacga gtactcgatc tttggtctgg tggctggaaa aggcttgagg
      901 ggaacatggc atgccgagta gcatttcgaa ggatacggga ggctccaacc tggggagaac
      961 aagccgtccc ggacaatttc gaatgggccg ccagggccaa cgggttcgta attcctcaaa
     1021 ggtcatcgga cagcctcctc gaagttctca caccagccca tcataactcc tgctcgggcc
     1081 ttttgggaga gaatacggtt agaatgtttt cgctctggat tttgccaaca actccaacac
     1141 agtgaaatgg tggagtcctg atgaatgtgg atccctcatc ggaagaagaa tcttcgacca
     1201 attcccgggc cccccttatg tcgatcggtt tggcggtttt tgctgtcctc atggtaccgg
     1261 taattctttt ctctatgggg ccggatgggc ctgccaaagt cggggacgtg gtttttgtca
     1321 cggatcgtca tcgtgtacgg ctgatgactt ccgatggtga tgcgattgga ccaaatcccg
     1381 agacgtgtgt cctcgagcct cgcgtgcagt tagtggtcca gaaaatgggc ataccctcag
     1441 gaggctcgat gattgcggaa ccggttgtca cggaaaaggt cggacgacct ttttgctttc
     1501 cgggaaggcc aattgtcgtg tatgcccatc aggtgacctt acggccagat ctgtggggtg
     1561 gcctccgaga ctcactttcg catttcttct ctggtcgctg agcaggacgc ccacaccgtc
     1621 gtttctctcc aggcgctcag gagggtttga gcttatctga aaagcgttgc cggaacttcg
     1681 ccactttggg attaatgagg taggcgcagt aaggttgtga cgggttctgc acaaaatatt
     1741 cctggtggta ggcttcggcc atgtaaaagg gtgacgcggg ttccacctgg gtcacaatgg
     1801 gatcgtcgaa caacttggct tccgtcagcg tgcgaatgac ctcgtgtgcg gtcgcctctt
     1861 gtgctgagga atgggtgaaa atggcggagc gatattgggt gcccgcatca ttcccctgtc
     1921 ggttcagggt ggttgggtca tggatgccga aaaagatttc caggagttcc cggtaggtga
     1981 tgattccggg attaaaggtt atttgtatca cctcggcatg tcccgtgttt ccgctgcaga
     2041 cggcctcata agttggattg gggcgactac cgcccatata gccggattct acggactcga
     2101 cacccttcac ctgatcgaat acggcttcga ggcaccagaa gcagccgcca cccaatgtgg
     2161 cgagttcttt tcctgttgat gaggctatgg ccgagtcaga catgcgttac tccttgctca
     2221 tgaaaaaggc taaagttgtg cgattgaagt ggatggtttc aggtttatat cacgacctgg
     2281 tttcttgggc cactgctggt tggactggtg aataggcaag agtgaaattt ttccctgaaa
     2341 ttcttcgctt tactttttgt tcggcccctt tataggcttg gacgattttc ttttagtctg
     2401 ttgagtggtg aggagaggag caagtcatat gcgggttgtg tgggccacgg atatccactt
     2461 gaattttctg ggtgtcgagg gacgggacgc atttttttta tccattcgtg ctcaacagcc
     2521 ggacgtcgtg tttgtgacgg gagacattgc tgaggctcca tcgttgaccc atctgataca
     2581 tgaaatgcgt cgagccattc acgtgccgat gtatttcgtc ctggggaatc atgattttta
     2641 ctatggttcg atttcccaaa ttcgaaatga tttgaaatgc tggtgtcaga gccagccagg
     2701 actcatctat ctttcgacat cggggttggt cgaattgact ccgacgacgg cgttggtcgg
     2761 gcatgacgga tggggagacg ggcgctatgg aaactatcat ctgtccccgg ttaggctcag
     2821 tgatcaggag ttgatcgcgg attttcagga tttagatcgg gaggccgtat tacgcaaact
     2881 ccgtgcgttg ggagatgagg cggcttgcta tttgcgggat cgattggatg aggccttgtc
     2941 atcatatcag cgcgtcattt gcctgacgca tgtcccacca tttaaagaag cctgctggta
     3001 tcagggaaaa atggggaacg atgattggct accatatttt gcctgccagg cagttggcga
     3061 ggtgttgctg aatgtgtcgc gagaacgacc ggattgtcaa atcactgtct tgtgcggcca
     3121 cactcatcat gcggggaccg ttcacctgcg gccaaatctg cgggttctta cgggatctgc
     3181 cgaatatggt gctccgtgta ttcaagagag cttcgacctt gagaaattgt ttccccaatc
     3241 aatgttggta ggaggcggtg agggtgaagg aggccaatcg atagaacctg atgccgccgg
     3301 aaggccatca ggtcccatta aataaatctg gtccgttcgt ttggagtagg tacgcggcac
     3361 gagttggatc ttcccatcca ttgatagcgg tgccgggcga tgaaactgta taccatatcg
     3421 cgaagtggcc gggggatgat gatgaagcca tacagccaag gccaagcccc aggtaggcag
     3481 cgaagaattt tgagagctgc agtggatttt gtgaagacct tcccgtgttc cagcagtaaa
     3541 aaggtttcat aatcctgggt cgaaagatgc aggtgagtga gaatgtcctg tgccggttgg
     3601 gattgaagcg ttccgaattt aaataatcct cgggaatccc gctcgaggac aaaattgaca
     3661 aacgcattgc aaaaattaca caccccatca aagacaatca ccttctcgtg tttgccccag
     3721 tcatgaatcg gtaaggaaga tccctgtttc acgtaacatg cccttttctc tgatcgatct
     3781 tttatctgtt ttggtcgttc ggcaatcaat aacttccctt ccattataat acatgagagc
     3841 catacgggtt ttctaaggcg gtagcgccta accagccatg gaaggtcgac gtttgaaaag
     3901 t
//
LOCUS       DAORAQ010000233         3876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3291_length_3876_cov_69.547239, whole genome shotgun sequence.
ACCESSION   DAORAQ010000233 DAORAQ010000000
VERSION     DAORAQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_3876_cov_69.547239"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKM72_13335"
     CDS             complement(<1..440)
                     /locus_tag="PKM72_13335"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNP61822.1"
                     /translation="MDLPELKTSLHECQRCALSSGRTQVVFGTGHPQADIMFVGEAPG
                     FYEDQKGEPFVGAAGKLLNELLQSIGLSRADIFIANVIKCRPPNNRDPLPEEIETCKP
                     FLLQQIELIKPKLVCTLGNFATQTLLERKVGITKVRGQVIRMPN"
     gene            complement(475..1863)
                     /locus_tag="PKM72_13340"
     CDS             complement(475..1863)
                     /locus_tag="PKM72_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP61823.1"
                     /translation="MTRHILILSGNLPGLLTAFRLVPYGFRITILEHHRSFSPTTSSP
                     QFSESKEPEPPTSFQHLMTQPSPLILPRFYHATWALFQEMAFEHAAQSIQPVELEFGT
                     SEGLTETLSNPHGIATLHPMIRLAGFRGLPWADRWRLINFLEKKWEEPHSPDHHPDML
                     TVESWLNSTQQSTLARQRIWNPFCRLFLGCDVTQASLEYFLEVLSRFWLSKPNGPETF
                     LASPDMLDHLQQQLKEVLLKKGVTFYPSNEITLIQADTERIQAIVLDKGERLTADIYV
                     SPLSPPELLRLLPERAPARFSSFSHLAHLQESSGTTVRFTLNDTFLPPRLILNSGIFD
                     WITSQAVRDADGARTSITGINLTPSPSSGHTDDWLKETVWPHIKNMLHISPGQSLPSG
                     KPPVMHSAYPYLPCLAGFRTFRPLPHTPIPNLFLAGPWTASLLPPSLESTVLSASACA
                     RAVTEWVQTSSH"
     gene            complement(1860..2750)
                     /gene="hpnD"
                     /locus_tag="PKM72_13345"
     CDS             complement(1860..2750)
                     /gene="hpnD"
                     /locus_tag="PKM72_13345"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNP61824.1"
                     /translation="MKPMTPIEAQTFCTKYTKESGSNFYYSFLFLPQQRREAMYTIYA
                     FCKMVDSAVDEPAPGSHPIEEVRKWRQELTATYQGHPTQPVTTSLAAHLQTFDIPEAL
                     LQELISGVEMDLTTNRFATFADLYQYCYRVASVVGLICLKIFQTQSPAAEDYAVNLGL
                     AFQLTNILRDLKGDAEQNRIYLPLEDLQRFGYSEEALLGQQESPALVELVKFECERAH
                     TYYRQAQEILQTLPPTDQKSLVVSEIMRGVYSRILTQLEDQHYQVFGPRIRVAPIQRL
                     GIAANIWIRSFLSHNIAPSV"
     gene            complement(3194..3727)
                     /locus_tag="PKM72_13350"
     CDS             complement(3194..3727)
                     /locus_tag="PKM72_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNP61825.1"
                     /translation="MMTSTIGTVFTPEQPSRWNVLFAQEPDKDLEFDEGLEEDDLNQS
                     KPPSRKPLFWIVLLLVIGGVAYWALNSNTFLPQETATETTAITRNPKGNESKIGMTDP
                     TFHENQQVTLTNILGRSLLMGDPANATPGPIVKLGETLTILDGAYQPTGWVYQVRTQT
                     GKTGWISADKLEPQAKK"
BASE COUNT          942 a          835 c         1101 g          998 t
ORIGIN      
        1 aagttgggca ttcgaatgac ctgcccgcgt actttggtga taccgacttt tctttccagt
       61 aacgtttggg tggcaaagtt tcctaacgtg cataccaatt taggtttgat gagttcgatt
      121 tgttgaagca ggaacggttt gcatgtctcg atttcttccg gaagaggatc tcgattgttg
      181 ggtggccggc atttaatgac gttggcaatg aaaatatctg cgcgactgag cccgattgat
      241 tgcagcaact cgttgagtaa tttccctgcc gctccgacaa aaggctctcc cttctggtct
      301 tcataaaaac ccggggcttc tccgacaaac atgatgtcag cctgaggatg tcctgtccca
      361 aacaccacct gagtgcgtcc tgaagacaat gcacatcgtt ggcattcgtg aagggatgtt
      421 ttgagttcag gaagatccat tgagggggga tcttaaattt gacggtttag gatgtcaatg
      481 tgaagaggtc tgaacccatt cagtcacagc ccttgcacag gcagaggcac tcaagacggt
      541 gctttccaga gaaggaggga ggaggctggc cgtccaagga ccggcaagaa aaagattggg
      601 aatgggcgtg tggggcagag ggcgaaatgt gcggaaacct gccaggcaag gaagataagg
      661 gtaagctgag tgcatgacgg gtggcttacc ggacggcaag gattgcccag gagaaatgtg
      721 aagcatgttt ttgatatgag gccagaccgt ttctttcagc cagtcgtcgg tatgtcctga
      781 ggatggcgaa ggggtaaggt taatgcctgt gatcgacgtc ctggcgccat cagcatcccg
      841 cacggcctgg ctggttatcc aatcaaatat accggagttg aggatgaggc gaggaggaag
      901 gaatgtgtca ttcagggtga accggaccgt tgttcctgag gattcctgca ggtgagccaa
      961 atgggaaaaa gaagaaaaac gagccggggc tctttccggc aagagtcgaa gaagctctgg
     1021 aggggagaga ggcgagacgt agatgtcagc ggtgagacgt tcgcccttat ccaggacaat
     1081 ggcttggatg cgttccgtgt cggcttgaat gagggtgatt tcatttgagg gataaaacgt
     1141 cacgcctttt ttaagtaaga cttctttaag ctgttgttgt aggtggtcga gcatgtcggg
     1201 tgaggctaga aatgtctctg gtccattggg tttggataac cagaatcgcg agagtacctc
     1261 cagaaaatac tcgagcgatg cctgggtcac atcacatcct aaaaataatc gacaaaatgg
     1321 gttccagata cgttgccggg caagagtcga ttgttgagtc gaattcagcc aggattcaac
     1381 tgtgagcatg tcagggtggt ggtccggtga atggggctct tcccattttt tttctaaaaa
     1441 atttatcaaa cgccatcgat cagcccaggg aagaccgcga aagccggcga gtcggatcat
     1501 gggatgaaga gtcgcaattc cgtgcgggtt ggatagcgtc tctgttaagc cttcggatgt
     1561 cccaaactcc agctcaaccg gttgaatgga ttgggctgca tgttcgaagg ccatttcctg
     1621 aaaaagtgcc catgtagcat ggtaaaaccg gggaagaatg agcggggagg gctgtgtcat
     1681 gaggtgttga aaggatgttg gaggttccgg ttctttcgat tcggaaaatt gaggagagga
     1741 tgttgtcgga gaaaaagatc tgtggtgttc gagaatggtg atccggaatc cgtaggggac
     1801 gagccgaaaa gccgtgagta atccgggcag atttccactc agtatcagaa tgtggcgtgt
     1861 cacactgatg gggcaatgtt atgggagaga aatgatcgaa tccagatatt ggcggcaatc
     1921 ccgagtcgtt gtatgggggc gacccgaatc cgtgggccga acacctgata atgttggtct
     1981 tcgagttgtg tgaggatgcg gctgtagacc ccacgcatga tttcggaaac caccagggac
     2041 ttctggtcgg tggggggaag ggtttggaga atttcttgag cttggcggta ataggtgtga
     2101 gctctttcgc attcaaattt cacaagttca accagcgctg gcgattcttg ttgacccagg
     2161 agagcttcct ccgagtagcc gaatcgctgc aagtcctcta gcggaagata gatgcggttc
     2221 tgttcggcat cccctttgag atcgcgaaga atattggtta attgaaacgc caatcccaaa
     2281 ttgaccgcat agtcttctgc cgcaggggat tgggtttgga atatcttgag gcaaatgagt
     2341 ccaaccaccg acgcgaccct gtagcagtat tgatagagat cggcaaacgt cgcaaaccga
     2401 ttggtcgtca aatccatttc gacgccagaa attaattctt gaaggagtgc ttccggaata
     2461 tcgaaggtct gtaggtgggc ggcgaggctc gtcgtgaccg gttgggtcgg gtggccttga
     2521 taggtggccg tcaattcttg tcgccatttg cggacttctt cgatgggatg gcttcccggc
     2581 gccggttcat caaccgcgct atccaccatt ttgcaaaagg cataaatggt atacatcgct
     2641 tcccgccgct gctgtggcag gaacagaaaa gagtaataga aattgctgcc actttctttc
     2701 gtatattttg tgcaaaacgt ctgcgcttca ataggagtca tcggcttcat gaagtatttt
     2761 ccaaaggcgg tggtcagtca atgatcgtga actggcggta caaatgaatg gcggcaactt
     2821 ttcaatttca aggcatgtta tgtcggatta agtcgctaac gaggttcccg cgccgccctg
     2881 gagggaaatt ttgagtcccc cttttctacg cataactctg ggtgactcat ctattgatca
     2941 acaagctcaa tatggcaaca atccgattgc cggatacaca actgataaac gtcagtgcag
     3001 agtagcaagg attgcgaagc tcttcaatgg atgggaagag ggtacttttc tggattgagc
     3061 gggatgtttt ccaggtgatt caacagcaag ccggatgatt ctcggcaatc gactccggct
     3121 ccttaagtgg aacaagtcct gccatcccag taaaaaaatg ggaaggttcc tccttaccgg
     3181 gtgggaacct ctcctatttt tttgcctgtg gttcgagctt atcggcagag atccagcctg
     3241 tttttcccgt ttgtgttcga acctggtaga cccatccggt tggctgatag gctccatcca
     3301 gaatcgttaa tgtttctcct aatttcacga tgggaccagg cgtggcgttg gcaggatctc
     3361 ccatcagcaa ggaccttcca agaatattcg taagtgttac ctgctgattc tcgtgaaagg
     3421 ttggatctgt cattccaatc ttggattcat taccctttgg attcctggtg atggcagttg
     3481 tctcagtggc ggtctcttgg ggcaaaaagg tatttgaatt gagggcccaa tacgctactc
     3541 caccaatcac caataaaagg acgatccaga aaagcggttt ccttgaagga ggcttggatt
     3601 ggttgaggtc gtcctcttcc aatccttcat cgaattccaa atctttatca ggctcctggg
     3661 caaagagcac gttccatcga gaaggttgtt ctggggtgaa caccgtaccg attgtcgagg
     3721 tcatcatgcc tatctccttt ttattcgtga ggtctgcaag cgagtgtcag tgccggattg
     3781 gcgtgggatg aatttgccaa atcagcgggt tgtgtacgaa gaaataccca tccatgaagt
     3841 ttattaacat cacttctatt tcctgtcaaa tcttcc
//
LOCUS       DAORAQ010000234         3871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3296_length_3871_cov_67.691038, whole genome shotgun sequence.
ACCESSION   DAORAQ010000234 DAORAQ010000000
VERSION     DAORAQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_3871_cov_67.691038"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="PKM72_13355"
     CDS             <1..1782
                     /locus_tag="PKM72_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNP61826.1"
                     /translation="TYHGQIDDSPTASGGLRFVRPDDTNGHIGPNGVYLTYETFWFPT
                     WEQTLSTYDLTLRLPSDWEAITQGREVANAVTDGHRTTQWRVNLPSEALTLAANHFVV
                     QKQEWKGIQLATYLYPEDTHLATQYIEATINYLQMYTELLGPYPFTKFAVVENFFPSG
                     LGLPSFTLLGEGVIRRGYTQPYSLGHEIVHSWFGNSVFNNSAQGNWVEGLTTYLSNYY
                     YDEARGNQQEAFNTRRRMVYEYNLYAEPDKEYPVRAFHHKETRLDNAIGYQKTAMAFH
                     MLRQEMGDVAFFKGVRGIIQEGTGTYLEWNDLLRIFSKAAERDLGWFFHQWIDQPGAP
                     TLDIQDILIQEDPTQQGRFTLTGTILQDEPVFTIRLPLHLHLQGERIHEAVLNVNQAA
                     QPFTLHLPANPTTLAIDPEHHLLLGLHRAQIPPMLNRWETDTRRILIRPQTMTTDEAQ
                     SLETLLQRLETQPGIETIQSDDPVISESASYLVIGPSAQRLLESGSFNNCEKSMDIHP
                     GHISIQEQTFEGPEMAFLISCAHPRDANHTVTFFFGWSPDAVAPVSRLLFFYGWDSYL
                     VFQQGKVIARGMFQPVHSAREIIRHSP"
     gene            1792..2901
                     /locus_tag="PKM72_13360"
     CDS             1792..2901
                     /locus_tag="PKM72_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61827.1"
                     /translation="MHPTFSIHPSKISIQNMLLFSLLSFLLLWNTALAGEPPAKQDQP
                     EPHLANIRQLTFSGKNAEAYFSAEGDRLVYQSTLEPDGKTLRSCYQIYIMDLDGTNVR
                     RVSLGLGGSTCGYFFPGGRRVLFSSTHIMSQFCPPAPERTERYRWALDDYDIFSMNIG
                     GRDLQRLTSAQGYDAEATISPDGKRIVFTSIRDGDLDLYTMDIDGKNLKRLTQEVGYD
                     GGAFFSPDNKRIVYRAHHPKTDEDLKAYRDLLSQNLVEPSNLEIFIMNADGSDKIQVT
                     HNGRSNFAPFFAQDGKRIIYSSNLSTPPDHQGRPSFHLHIINEDGTHPEQITFNGHFN
                     SFPMFSPDGKQVVWSSDRNAANPKEFNIFLADWVP"
     gene            3141..>3871
                     /locus_tag="PKM72_13365"
     CDS             3141..>3871
                     /locus_tag="PKM72_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNP61828.1"
                     /translation="MTDQSPASPSPRRSQFLLPQSPLVVFGLSGLLFFGLFASLALVD
                     GPTIQIVHSFTHPAIDHIGNVGNHLGDGLTLVLISLGIGAVGFRLGWPTWKSACIHTL
                     LAHGVAGLFTQILKHTIGRPRPRLKQGQDWEIAPSFESGWDSFPSGHTTASFSVATVL
                     AHYFPNARYLWFGLAAFGGICRVITGAHFPTDVLGGILVGIGTALIIIHPPHKWKRLS
                     QHTLTHGLPWLVTAFGMVWIIVPHPG"
BASE COUNT          921 a         1106 c          894 g          950 t
ORIGIN      
        1 acgtaccatg ggcagattga tgattctcca acagcctcgg gcggacttcg ctttgtcaga
       61 ccggatgaca ccaacggaca tattggaccc aacggcgtgt atctgacgta tgaaacattc
      121 tggtttccca cctgggagca gaccctctca acgtatgatc tcaccctccg cctcccttca
      181 gactgggaag cgatcaccca gggccgggaa gttgccaatg ccgttaccga cgggcaccga
      241 accacccaat ggagagtaaa ccttccgagt gaagcgttga cgttggctgc caatcatttt
      301 gtggtgcaga aacaggaatg gaaaggcatt caactcgcca cctacctcta cccggaagac
      361 acccacctgg ctacgcaata tatagaagcg accatcaact accttcaaat gtataccgag
      421 ctcctgggtc cttacccgtt tacaaaattt gctgtcgtgg aaaatttttt cccaagcgga
      481 cttggactcc cctcattcac cctcctagga gaaggagtga ttcggcgcgg atatacccaa
      541 ccctattctc ttggccatga aatcgtccat tcctggttcg gaaattctgt ctttaataac
      601 tctgctcaag gcaactgggt ggaagggctc accacctatc tctccaatta ctattatgac
      661 gaggccaggg gcaatcagca ggaggccttc aacacccgac gacggatggt ctatgaatat
      721 aatctgtatg cggagccgga caaggaatat ccggtacggg ccttccacca caaagaaacc
      781 aggctggata atgccattgg gtaccaaaaa accgccatgg catttcacat gcttcgccag
      841 gaaatgggag acgtggcatt tttcaagggg gttcgcggga ttattcagga agggaccgga
      901 acctatctgg agtggaacga tcttctccgg atattttcaa aagccgctga aagagacctc
      961 gggtggtttt ttcatcaatg gatcgatcaa cccggtgctc caaccctgga tatccaggac
     1021 atcctcatcc aggaagatcc cacgcaacag ggacgattca cattgacggg aacaatcctg
     1081 caggacgaac ccgtattcac catccgtctt cccctccatc tccatctcca aggagaacgc
     1141 attcatgaag ccgttctcaa tgtgaatcag gccgcacaac cttttaccct gcatctcccc
     1201 gcaaatccaa cgacactcgc cattgatccc gagcaccatc ttctcctcgg ccttcaccgt
     1261 gcacaaattc cgcccatgtt gaataggtgg gaaaccgaca cccgtcgcat tctcatcaga
     1321 cctcagacca tgaccacgga tgaggcgcaa agcctggaaa cgctgcttca acgtcttgag
     1381 acacaacccg gcattgagac catccaatcc gatgatccgg ttatttctga atcggcctca
     1441 tatctggtca tcggaccctc tgcccaacgc ttattggaat ctggttcctt caataattgt
     1501 gaaaaaagca tggacattca tccgggacac atctccattc aggaacagac attcgaaggc
     1561 ccggagatgg ccttcctgat ttcttgcgcc catccccggg atgcgaacca taccgtcaca
     1621 ttttttttcg ggtggtctcc tgatgccgtt gcgcccgttt cacggctgtt gtttttttac
     1681 gggtgggata gctatctggt tttccaacaa ggaaaagtga ttgcccgggg gatgtttcaa
     1741 cccgtacact ctgcacgcga gattattcgc cattcaccgt gaggttcatg aatgcatccg
     1801 accttctcta ttcatccatc aaaaatttcg atacaaaaca tgctcctgtt tagcctgctg
     1861 tccttcctgc tgttgtggaa tacggctctg gccggggaac cccccgccaa gcaggatcaa
     1921 ccggaaccgc atctggccaa tatccggcag ctcacgtttt cagggaaaaa tgctgaagcc
     1981 tatttttcag ccgagggtga ccggttggtg taccaatcaa ccctggaacc ggatggcaaa
     2041 acgctgagat cctgctatca aatctatatc atggacctgg acggtacaaa tgtccggcga
     2101 gtcagcctcg ggctgggcgg ctccacatgc ggatactttt tccccggtgg ccggcgtgtg
     2161 ctcttttcct ccacccatat catgagtcaa ttttgtccac ccgcccccga gaggacggaa
     2221 cgctaccggt gggccctgga tgattatgac atcttctcca tgaatatcgg cggacgggac
     2281 ttacaacggt tgacctccgc ccaaggctac gatgcagaag ccaccatctc cccggatgga
     2341 aaacgcattg tcttcacttc catacgtgat ggggatttag atctgtacac gatggatatc
     2401 gatggaaaga atctgaaacg gctgacgcag gaagtcgggt atgatggcgg agcctttttt
     2461 tctcccgaca acaaaaggat tgtctatcgg gcccaccatc cgaaaactga cgaagacctg
     2521 aaggcctatc gtgacctgct cagtcaaaac cttgtggagc cgtcaaacct ggaaatcttc
     2581 atcatgaatg ctgatggttc cgacaaaata caggtcactc acaacgggcg atcgaatttt
     2641 gctcccttct ttgctcagga tggaaaacgc atcatctatt cctccaactt gtcgaccccg
     2701 cccgatcatc aggggcggcc atcctttcac ctgcatatca tcaacgagga tggcacgcac
     2761 cccgaacaaa ttaccttcaa tgggcacttc aatagttttc cgatgttctc gccagacggg
     2821 aaacaggtgg tatggtcatc cgatcggaat gccgccaacc caaaagaatt caatatcttt
     2881 ctcgctgact gggtaccgta aatcatcttg aattttttcc tcctttgtaa ggaggggtga
     2941 tggggaagta gaaacatgca aaaatcaact agtctcattt tgcggtatgc tctcttcttt
     3001 tcgtatcgct accccacctc tttcattcct cttcggaatg aaacccctta caaaggggag
     3061 gattcagaaa ccaaaatcct ctctgtgcgg gccttcctgc cagggtagaa atccatccat
     3121 atggttctcg ctagtatcct gtgactgacc aatcacccgc atctccttca cctcgacgtt
     3181 cgcaatttct cctcccccag tcccctcttg tggtattcgg actatccggg ctcctctttt
     3241 ttggcttgtt cgcttctctg gctctggtcg atggcccgac catccagatc gttcacagtt
     3301 tcacgcatcc ggccatcgac cacattggca atgtaggaaa tcatttagga gacggactga
     3361 ctctggtgct catttcattg ggaattgggg cagtaggatt ccggcttgga tggccgacct
     3421 ggaagtcggc atgtattcat accttactcg cccatggcgt agccggactt ttcactcaaa
     3481 tattgaaaca caccatcggc cgtccccgcc caagactcaa gcaaggtcag gactgggaaa
     3541 tcgccccgtc atttgaaagt ggatgggact cctttccatc cggtcatacc accgcttcat
     3601 tttccgtggc cacggttctt gcgcattatt ttcccaatgc ccgatacctg tggttcgggt
     3661 tagccgcgtt tggtggaata tgccgtgtca tcaccggcgc ccattttcct acggatgtgc
     3721 tagggggaat actggtgggg atcggaaccg ccctgatcat cattcatcct ccacacaagt
     3781 ggaagaggct ctctcagcac acactcacac atggactccc ctggctggtc acggcatttg
     3841 gaatggtttg gattattgtt ccccatccag g
//
LOCUS       DAORAQ010000235         3869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3297_length_3869_cov_67.226009, whole genome shotgun sequence.
ACCESSION   DAORAQ010000235 DAORAQ010000000
VERSION     DAORAQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3297_length_3869_cov_67.226009"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKM72_13370"
     CDS             complement(<1..237)
                     /locus_tag="PKM72_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNP61829.1"
                     /translation="MNHIRQLEDQSVYILREAYKNFDNLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSFKIPEMIEYRDRLAREWRL"
     gene            complement(234..1322)
                     /locus_tag="PKM72_13375"
     CDS             complement(234..1322)
                     /locus_tag="PKM72_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61830.1"
                     /translation="MDHLIAKIGKGLKGAKDLTWDEAKFALNAIIEGQATDVQVGAFL
                     MAMRIKTESISELAAFTTTVRKWAPPIPLPHAENIVDVPLYGEKHNTIHVVVAASLVA
                     ASAGAGLFMHGVENPDTPYDLPQVLHHLQLPIARTAAEAIEQGAQSPWVYMDLAGYHA
                     PLTKLLDLRQAFGLQNLSHQVSRLLNPARVGSQVIGISHPPYLDKMVEALDMLGAKRA
                     LIIQGVEGFPELSLSAPSMARELRSGHISSLVFRPDDAGFRSGPFQAMSSTINGPGSG
                     TPEHEARLMIQVLVDRQKGDRRNWVIMNAALLMYAGGLASSLAQATPLAQETLDSGKA
                     GAFFTALVQGRPPQSKFVVPPSPAVVPA"
     gene            complement(1337..1750)
                     /locus_tag="PKM72_13380"
     CDS             complement(1337..1750)
                     /locus_tag="PKM72_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNP61831.1"
                     /translation="MKKIAVIITKGGWNNLFQACEWIALAAASGMEVSGYFRDEAAGR
                     MTKDKIKELTMSPDFKGREGFVRDLLKKEDKTDLPKMMQTSKEKGNVKFSVCRDSLKY
                     FGVNVEELIPELDEVQTAEAFWKEAVLPADQVLTF"
     gene            complement(1827..2543)
                     /locus_tag="PKM72_13385"
     CDS             complement(1827..2543)
                     /locus_tag="PKM72_13385"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNP61832.1"
                     /translation="MALFLNTHPTPEELVAVNRSMEGQAPRQIVETAIAEYGGTVILA
                     CSFGAEDVVLADMMFRSNPGSTLFYLDTDFLFPETHAVRDRIIQHYQLQDDQIIQVKS
                     TLSPSEQATQFGEALWQREPDQCCSLRKVEPLTRILAKYAAWMTGIRRDQSPTRANAR
                     IIEWDTKFGLVKFNPLATWSWEQVWEYIRMNDVPYNALHDRHYPSIGCTHCTAPVMPG
                     EDPRSGRWKSSEKTECGLHR"
     gene            complement(2550..>3869)
                     /locus_tag="PKM72_13390"
     CDS             complement(2550..>3869)
                     /locus_tag="PKM72_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNP61833.1"
                     /translation="MVRWVPDIRLRHFYDELVAHGLGDPGAEGVADIVSCPGTDTCGL
                     GITSSKGLARALAEVFPAGKIAEDLEGVNVKISGCHNSCAQHHIATIGLHGVGKRIGD
                     HVAPVYELHLGGRVNGTAKIGQLIVKVPAKNVPAAVQHLLDLYRRDKNNGESLLGFID
                     RTGKIKLKDELIPYTILPTYQEDPQFYVDWEGDEEFSVEDLGPGECAGGALEMIDNRI
                     LEAEQELYQARILAEKHQYAFAINKAYRAVVAGAKAILVTEGIDPNTDADTLAEFNKL
                     IVAKGLMPAEYHNLASIVGDLGNKDASADLTQGKIAFTKGFVDLCRTVSDQIGQDLKL
                     APAELGQKPTESTKEATTPPEKASSPSGVPVYDLRGVACPMNYVKTKLKLEMMDHGDQ
                     LEVWLDGGDPIRNVPVSLRNDGHNVLEQEPMDADEQHFRVVVEKVEL"
BASE COUNT          862 a          992 c         1020 g          995 t
ORIGIN      
        1 taatcgccac tcgcgtgcga ggcgatcccg gtattcaatc atttcaggaa ttttgaagct
       61 ggtgtcaaca tggagtaagg ggaaggggac atggccgaaa aacgcttttc tggccagcca
      121 caataacacg gttgaatcct tgcccatgga ccagagcatg gccaggttat caaagttttt
      181 atacgcctca cgcagaatat agacgctttg atcttctaat tgacgaatat ggttcatgcg
      241 ggcaccaccg ccggtgaagg ggggacgaca aattttgact gtggggggcg tccttggacc
      301 aaggcggtaa agaacgctcc ggcttttccg gagtccagtg tctcctgggc gagcggtgtg
      361 gcttgcgcga gagaggaggc caaccctccg gcatacatca gtaaagctgc attcataatt
      421 acccagttcc gtctgtcgcc tttctggcga tcgacgagta cttgtatcat caatcgggct
      481 tcatgctcag gggtgcccga tcccgggccg ttaatggttg aactcatggc ttgaaatggt
      541 ccggatcgga acccggcatc atcgggacga aagaccaacg atgaaatatg tccacttcgt
      601 aattcacgag ccatggaagg tgcagaaagg gacagttcgg gaaatccttc gactccttga
      661 atgatcagtg cccgtttcgc tcccagcatg tccaaggctt ccaccatttt atccagatat
      721 ggcggatggg aaatgccgat gacttgtgag cccaccctgg cagggttcaa tagtctggat
      781 acctgatggg acaaattttg taaaccgaat gcctgacgca ggtccagcaa tttggttaat
      841 ggtgcatggt atccggctaa gtccatatac acccagggcg attgagcgcc ttgctcaatg
      901 gcttctgccg cagtacgggc gatgggaagc tgtaagtgat gaaggacttg agggagatcg
      961 tacggggtgt ccgggttttc aacaccgtgc atgaacagtc ctgcgccggc cgaagctgcg
     1021 acaaggcttg ccgcgacgac cacatggatg gtgttatgtt tttctccata gagcggcacg
     1081 tccacaatgt tttcggcatg cgggagtggt atcggtggtg cccacttgcg aaccgtggtg
     1141 gtgaacgcgg cgagttcgct gatggattcg gttttgatac gcatggccat caaaaaggct
     1201 cccacctgaa cgtcggttgc ctgcccttca atgatggcgt taagcgcaaa tttggcctca
     1261 tcccaggtta aatccttggc acccttcagc ccttttccaa tttttgcaat caggtgatcc
     1321 atgcgatgat tccgggttaa aaggtcaaaa cctggtcagc gggaagaaca gcttctttcc
     1381 agaaggcctc ggcggtttgc acttcatcta attccggaat gagttcttcg acattcactc
     1441 cgaaatattt cagagaatct ctacacaccg aaaatttgac gtttcccttt tctttagagg
     1501 tctgcatcat tttcggaagg tccgttttgt cctctttttt caagagatca cgcacaaagc
     1561 cttcccgtcc tttaaaatcc ggggacatgg tcagctcttt gattttgtcc ttggtcattc
     1621 ggcctgcggc ttcgtcacgg aaatacccac tcacttccat gccgctggcg gctgccaggg
     1681 ctatccactc acaggcttga aacaagttgt tccatccccc ttttgtaata atgacggcaa
     1741 tttttttcac tgagggtctc cgatagttac ttgattgagg aaaatgaatg taaagtcatg
     1801 aacgacgaaa tggtcacatg gccggattat cgatgaagtc cacattcggt tttctccgaa
     1861 cttttccatc gtccggagcg tgggtcttct cccggcatga cgggcgcggt gcaatgggta
     1921 catccaatac tgggataatg ccgatcatgc aaggcattgt acggaacgtc attcatgcga
     1981 atgtattccc agacctgttc ccatgaccag gtggctaagg ggttaaattt tacaaggcca
     2041 aatttggtat cccactcaat gatgcgggcg ttggcgcgag tgggtgattg atcacgccgg
     2101 attcccgtca tccacgcggc atactttgcc aggatgcgag tgaggggctc aacctttcgc
     2161 agggagcagc attggtcggg ctcccgttgc cataaggctt ccccaaactg tgtcgcttgt
     2221 tcggaaggac tgagtgttga tttaacctgg ataatctgat catcctgtaa ttgataatgt
     2281 tgaatgattc gatcccgaac agcatgggtt tcaggaaata aaaaatcggt atctaaataa
     2341 aagagtgtgc tgcctggatt tgagcggaac atcatatcgg ccaggacgac atcctcggcc
     2401 ccaaaactac aggccagaat aaccgttccc ccatattccg cgatcgcggt ctcgacaatc
     2461 tgtcgaggcg cctgaccttc catggatcga ttgaccgcga cgagttcttc gggagttgga
     2521 tgggtattga ggaaaagggc catggcgttt tacagttcca ctttctccac taccaccctg
     2581 aagtgctgct cgtcggcgtc catgggttcc tgttcgagca cgttgtgccc atcgtttcga
     2641 agactgaccg ggacgttgcg aataggatcg ccgccgtcca accatacttc caactggtca
     2701 ccgtggtcca tcatttctaa tttgagcttg gttttcacgt agttcatggg acatgccacg
     2761 ccccggaggt cgtaaacggg cacacctgat ggggaggatg ccttttcagg tggcgtggtt
     2821 gcctccttgg ttgattcagt gggcttttgt cccagttcag cgggagccag cttgaggtcc
     2881 tggccgatct ggtctgagac ggtccggcag aggtccacaa aacctttggt aaacgcgatt
     2941 tttccctgtg tcaggtcagc cgaggcgtcc ttattcccca gatcgcccac tatagaggcc
     3001 agattatggt attccgcagg catgagccct ttggccacga tcaacttgtt aaattcggct
     3061 aaggtgtcgg catcggtatt cggatcgatt ccttctgtga caaggatggc tttcgctcct
     3121 gccaccacgg ctcgataagc cttgttaatg gcaaacgcat actggtgttt ttcagccaaa
     3181 atgcgtgctt ggtacagttc ctgttccgct tcaagaatcc gattgtcgat catttccaag
     3241 gccccacccg cacattcgcc gggtccgagg tcctcaaccg aaaattcctc gtcgccttcc
     3301 cagtcaacat agaattgtgg atcctcttga taggtgggaa ggatggtgta ggggatcagt
     3361 tcatctttca gtttgatttt tccggtgcgg tcaataaaac ctaataatga ctccccatta
     3421 tttttgtccc ggcgatagag atcgagaaga tgctggacgg cagcgggaac gtttttcgca
     3481 ggaaccttca caatgagttg gccaattttg gctgtgccgt tgacccgtcc cccaaggtgc
     3541 agttcgtaca ctggcgcgac atggtctcca atacgtttgc cgactccatg taagccgatc
     3601 gtggcgatat gatgttgcgc gcaggaattg tggcagccac tgattttcac gttcacccct
     3661 tccaaatcct cagcgatttt cccggcaggg aatacttctg ccagtgctcg tgccagtcct
     3721 ttagaagagg tgattcccag tccgcaggtg tcggtccccg ggcaggaaac gatatcggct
     3781 actccctcgg ctccagggtc ccccaatcca tgagcgacta attcgtcata gaagtgccgg
     3841 aggcgaatat cgggaaccca acgtaccat
//
LOCUS       DAORAQ010000236         3852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3317_length_3852_cov_60.914406, whole genome shotgun sequence.
ACCESSION   DAORAQ010000236 DAORAQ010000000
VERSION     DAORAQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_3852_cov_60.914406"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1213)
                     /locus_tag="PKM72_13395"
     CDS             complement(323..1213)
                     /locus_tag="PKM72_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61834.1"
                     /translation="MKNVKKWRVIKNENKVLACVDQSHFADYVADYAAWAACRMGAPL
                     EFLHVIDRHPEIATGHDHSGAIGIDAQEVLLNELSNEDESRSKAARERGRIFLNELRE
                     RAMVSGVESPDVRQRYGLLEETLVEQEESVRLFVLGRRGVSAEATQRDLGRNVERVVR
                     ALHKPILAVTDSFSEPRRVMIAFDGGIVTRRGVEMVAASPLFRGLPVDLLMSGKESQS
                     ASKQLEWAKTTLEAASFDVSPSLIPGDAERVIAKAVHEQGIDMLIMGAYSHSPLRSLV
                     FGSKTSDLLRSAKVPTLLLR"
     gene            complement(1228..2712)
                     /locus_tag="PKM72_13400"
     CDS             complement(1228..2712)
                     /locus_tag="PKM72_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNP61835.1"
                     /translation="MIQEIVGKIREGMNIRNDLLAGLVVALALIPEAIAFSVIAGVDP
                     KVGLYASFCMATVIAFAGGRPGMISAATGAMALLMVTLVKDHGLQYLLAATVLTGVLQ
                     VVAGWLRLGSLMRFVSRSVITGFVNALAILIFMAQLPELTGVSWEAYAMVGAGLAIIY
                     LFPYLTKSVPSPLVAITVLTGVSIGLGLDIRTVGDMGQLPDTLPIFLLPDVPLHWDTL
                     RIIFPVSATLAVVGLLESMMTASIVDDLTDSSSNKNRECVGQGIANMTSGFIGGMAGC
                     AMIGQSVINVKSGGRGRLSTLVAGVFLLLMVVYIGDWVARIPMVALVAVMIMVAIGTF
                     NWASIRNLREHPKSSNVVMIATVVVVVATHDLAKGVLVGVLLSGFFFAHQVGQILHIG
                     WRSENQGRLRTYIITGQVFFASAERFVNSFDFKEVIEKVQIDVSRAHFWDLTAVSALD
                     KVILKFRREGTEVEVIGLNEASATMVDRFAVHDKPDAVEQVMGH"
     gene            complement(3031..>3852)
                     /locus_tag="PKM72_13405"
     CDS             complement(3031..>3852)
                     /locus_tag="PKM72_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61836.1"
                     /translation="VVNREGKEFLLLVAQTAQPNQVNLLISASALEKQGYLNHPDQLV
                     PVLAREFQWVVSKSDTTSKRQTNMPPGNFKQVPIKTNQEIAEMSGEDRDHLLQLLFRT
                     YLTTTDRHNSLDGQSYYETGSTTRIPPAQSDSTTKLYDIRVREALQKIVREPYFQEQT
                     PKAIRSLLNGKVWNVTFADIQNRDWATRTRVVPKEQSITVGEQERTIQPAMILINIHR
                     SAAPEDPFYTETNGLPMGALPVDKLARVIAQEIQNNITEKSMRGHVAQDELSAPQ"
BASE COUNT          885 a         1114 c         1010 g          843 t
ORIGIN      
        1 gccttcagga gaccacggag ggtttgcaca aaaaaatcgt gtaagtccct gtttcattct
       61 cgaggggtct gtcgccccgg aaagaagagg tcttacacga tacgtttgac ggtatcatag
      121 ggtttgtatt ctgacaagct ggacctatga cagcccggct tccttttttc ttctacgtcc
      181 accccttcgt catgatgaac atcaccgagg gttcccgtcc tccattccgt ggctgcgccg
      241 tgtctgtcac cagtcaccgc ctctccgcat tcactatttt tatttggtgg tatcagattg
      301 aaacgaagca acggcagatg atttatcgga gcaatagcgt cggaacttta gccgaacgca
      361 acaaatcgga ggttttgctg ccgaatacca gactgcgaag cggagaatgg ctgtacgctc
      421 ccatgatcag catatcaatg ccctgctcat ggaccgcttt ggcaatgacg cgttccgcgt
      481 cgccgggaat gagtgaaggc gaaacatcaa agcttgccgc ctccaatgtg gtcttggccc
      541 attcaagctg tttggaggca ctctgacttt ctttgcctga catcagaagg tccaccggca
      601 ggccacggaa gagcgggctg gccgccacca tttcgacacc tcgtcgggtg acgattccgc
      661 catcgaaggc aatcatgacc cggcggggct cgctgaagct atcggtgacc gcgagtatcg
      721 gtttgtgcag ggcgcgcacc acccgctcaa cattgcgccc caaatcacgc tgggtcgcct
      781 cagccgagac accccggcga cccaacacaa acagacgcac ggattcttcc tgctccacca
      841 aggtctcttc caacagcccg tagcgctggc gaacatcggg agattcgact cccgagacca
      901 tggcccgctc ccgtaattcg ttcaagaaaa tccggccacg ctcacgggcc gccttgctgc
      961 gggactcatc ctcattggac aattcgttca acagcacttc ctgcgcatcg atcccgatgg
     1021 caccgctgtg atcgtggccg gtcgcaatct cgggatgccg atcgatcaca tggagaaatt
     1081 ccagcggtgc ccccatgcgg catgccgccc aggcagcata gtcggccacg taatcggcga
     1141 aatgcgattg atcgacgcag gccagcacct tattctcgtt cttgatgacc cgccactttt
     1201 tcacgttttt caccagatgt gttcctctca gtggcccatc acctgttcca cggcatcggg
     1261 tttgtcatgg acggcgaagc gatccaccat cgtggcgctg gcttcattca ggccgatgac
     1321 ctcgacttcc gttccctcgc ggcgaaactt gagaatgact ttatccaacg cgctgacagc
     1381 agtcagatcc cagaagtggg cacggctcac gtcgatttga accttttcga tcacttcctt
     1441 gaaatcaaaa gaattgacaa accgctcagc cgaggcaaag aacacctgcc cggtgatgat
     1501 atacgtgcgc agacggccct gattctccga cctccagcca atatgcaaaa tctgtccgac
     1561 ctggtgagcg aaaaagaacc cggacaacag cacgccaacg agcacgccct tcgccaggtc
     1621 atgagtggcc acgaccacca ccacggtggc gatcatcacc acgttggagc ttttgggatg
     1681 ctcccgcaga ttgcggattg aggcccagtt gaaggtgccg atcgcgacca tgatcatcac
     1741 ggcaaccagt gcgaccatcg ggattcgggc cacccagtca ccgatgtaca ccaccatcag
     1801 caacagaaat acgcctgcaa ccagggtcga gagccgccca cggccgcccg atttcacgtt
     1861 aatgaccgac tgcccgatca tggcgcatcc ggccatgccg ccgatgaaac ccgaggtcat
     1921 attggcgatg ccctgtccca cacattcacg attcttgttg cttgaggaat ccgtcaggtc
     1981 atcgacgatg gaggcggtca tcatggattc cagcagcccc accacggcca gtgtggccga
     2041 caccgggaag atgattcgga gggtgtccca atgcagcggc acatccggca gcaggaatat
     2101 cggaagggtg tcagggagtt ggcccatgtc acccacggtg cggatatcca agccaagacc
     2161 aatggacaca ccggtcaaca ccgtaatggc taccagcgga gacggcacac tcttggtgag
     2221 gtatggaaag aggtagatga ttgccaggcc ggcgcccacc atcgcatagg cctcccagct
     2281 cacaccggtc agctcaggca gttgcgccat gaagatgagg atggccaacg cattgacgaa
     2341 gccggtgatc acggagcgcg acacaaagcg catcagggaa ccaagccgga gccagccggc
     2401 aacgacttgc agcacacccg tcagcaccgt agcggccaac agatattgca acccatggtc
     2461 tttgaccagg gtgaccatca acagcgccat cgcacccgtg gcagccgaga tcatgcccgg
     2521 ccgaccgccg gcaaaggcga tgacggtggc catacagaac gaggcgtaca gtcccacctt
     2581 ggggtctacc ccggcgatga ccgaaaaggc aatcgcctcg gggatgagtg ccagtgcgac
     2641 gacgagacct gcaagcagat cgtttctgat attcatacct tcacggattt tgccaacgat
     2701 ctcttgtatc attcatcatc ctttttcgat ttagacagac cttctgcaca aaaaatacac
     2761 gccaagagac cattgggggt gcgggctacg cccgaccacc ctttctgtcc catcacacaa
     2821 ctcctggatg cttgctggaa tgttcctgtt ttcaggagac caaggagggt tcgaacaaaa
     2881 aaatcgtgta aggccttact ttactttcaa gaggtctgtt gccccaggaa gaagaggtct
     2941 tacacgatac gtttgacagt atcacagact attcacacac acaagcggca ttgttgagtg
     3001 agaaaatgaa gattgggggc accgcactga tcattgaggg gcggagagtt catcctgggc
     3061 cacatggccc cgcatcgact tctccgtaat attattttgg atttcctggg caatcacccg
     3121 tgccaacttg tcgacgggaa gtgcacccat cggaagacca ttggtttccg tatagaaagg
     3181 atcttccggt gcggcagatc gatggatatt aatgaggatc atggcgggtt gaatggttct
     3241 ctcttgttcg ccgaccgtga tcgattgctc tttgggaacc acccgggtcc gcgtggccca
     3301 atcccgattc tgaatgtccg cgaatgtgac gttccaaact ttgccattga gcagactcct
     3361 gatcgccttc ggagtctgtt cctgaaagta tggttctcgc acaatttttt gcagcgcctc
     3421 acgcacccga atgtcataca atttggtcgt ggaatcagat tgtgccgggg ggatccgtgt
     3481 ggtggaaccg gtttcgtaat aggattgtcc atccaaactg ttatgcctgt cggtggtagt
     3541 taaataggtg cgaaacaaaa gctgaagcag atgatcgcgg tcctctcctg acatttccgc
     3601 aatttcttga ttggtcttga tcggaacctg cttgaagttt ccggggggca tatttgtttg
     3661 acgtttcgag gtcgtgtcgc ttttgctcac cacccattga aattctctcg ccaataccgg
     3721 caccagttga tccggatgat tgagatagcc ctgtttctcg agtgcggatg cgctgataag
     3781 cagattgacc tggttgggtt gtgcggtttg ggccaccaac aataaaaatt ctttaccctc
     3841 ccgattcacg ac
//
LOCUS       DAORAQ010000237         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3318_length_3851_cov_67.502107, whole genome shotgun sequence.
ACCESSION   DAORAQ010000237 DAORAQ010000000
VERSION     DAORAQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3318_length_3851_cov_67.502107"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKM72_13410"
     CDS             <1..210
                     /locus_tag="PKM72_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNP61837.1"
                     /translation="QTVQTLVNPIPVITIHMAKDLQGPQPDTDLIFAGPENFDDCTHQ
                     IIDLLKSRGILTEPPKGRADFQYSI"
     gene            complement(485..3523)
                     /locus_tag="PKM72_13415"
     CDS             complement(485..3523)
                     /locus_tag="PKM72_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61838.1"
                     /translation="MKNRRLSFSLTCLCFLLQACISSSTLLAESSNDTLQRKSIMIMS
                     PTNASAHTTAPITIQVRVAHHAAKAMQVLLDGVDITSRFMAPGRNGVRRAEVDRPLVN
                     WGKNQILVRSGSHEVNSHFFLVDHPDSSPAGANLPLLIPIKTRVVTGSGDKAADYNIA
                     LYKDLDDPTGATLIPASVPSDGSNAGFQVVFMRRWDLSVISNVSVVNQRPPSDGRWHD
                     SPLYQSIVYTPEDCLSAGCILLIQSLGTIGFTPCSKAYSKDFPKDCESFKNMLNGLGL
                     SSRLAYANGDSKQIAFSSISNKVPFPPTSGPPKPPPGTFFESLTCSGSNYGSGPACDS
                     LGSPNTSFTAPKGAEPSQLGDIAGVLIRDNHHNFTFARNDRLVSFSTATIPDGPDGVM
                     NTITVDGVPHASGSLNGSQGGFHLLILNSKDLTVVRHQTFPSKLDASEVNRLYDAITG
                     YKSYGYLFFIAGFGYTNYNCQSSTSTGLCGARVNWYTASQLMGDLGGTQQVFYLANNP
                     NADPYLYDDYTLVGSFVDGVGGESTDTGLDQVGAEMSSIIARETEAFPPPTGAGHGAD
                     MEGFLRVNHQGYYSPASFGHRLNLSKTTIADILSASLLNPTPWPFPGPDPAGSKAAYD
                     WISQQLCCVDIRSAYVNLNISPAIWLAELRHLTYDSSRIPNSSPADFDAMVQQLTTEF
                     QYVTLARLFQSNVIGLYQDQQANVSLLLQDDGDKILHNLQVGLTTQAHAASWTGILSD
                     VFGVGSSLLNAIPGGGIVAAGTEVALTVGTAIANQAASHTNSAAGTPLQAQENQEIAA
                     GDLANQLANQFSATLVSLGSEFDRVMTDWGRLKTLAAPLLADQVPWDNNATGLLLQSY
                     DRAVQRELYAQLLPTNFVVQHLPYTGNNLHVVNTYFDRGGSECEWEHFINNKAVLYYP
                     NGHINNDPNENSHSGKYPWNYQWGIWALTLAKYQNDSCPGHSQNYPKTYDYPDTFGLF
                     KPLDPGNPDALGAYRYWFYTRGSFPIIENNNNTPCYDGSC"
     gene            complement(3592..>3851)
                     /locus_tag="PKM72_13420"
     CDS             complement(3592..>3851)
                     /locus_tag="PKM72_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61839.1"
                     /translation="SKRKPGERAWNDITFNGAARWNSMNLEIIDADLAVIKFLSSHVP
                     SFFILLSVICIRDEERPIFVDSVRFFRYVGHGIRFRSSAEG"
BASE COUNT          924 a          994 c         1061 g          872 t
ORIGIN      
        1 caaaccgtgc aaacgttggt gaacccgatt ccggtcatca ccatccacat ggccaaagat
       61 ttacaagggc ctcaaccgga taccgatcta atttttgcag ggccggaaaa tttcgacgac
      121 tgcacccacc aaatcattga tttgctcaaa tccaggggga ttctcactga acctcccaag
      181 ggccgtgccg acttccagta ctccatttaa ggcaatcgtc atttcccgca aggatgactg
      241 actgtaccca ccattctcac tcgagatgtg ggtcaatcag ggacgaaact cgcccattca
      301 tccagacgta tcattttggt agacaagatt ccatccgttt tctttttttt aggcaccgtc
      361 gaactgaaaa cttgagtcac acgagtgtga aagacgatga gtagcaatta ttatttcgtc
      421 ccagtgagca catggacgtg tgagtcaggc tgcgaaggct tcatgtcatg acgacaattg
      481 cagatcagca acttccatcg tagcacggcg tgttgttgtt attttcaata attggaaagc
      541 tgcctcgcgt atagaaccag tagcgatagg cacccaaggc atccggattg ccgggatcca
      601 gcggcttgaa caggccaaac gtgtccggat agtcatacgt cttcgggtaa ttctgactgt
      661 ggccggggca tgaatcgttt tgatatttgg caagtgtcaa tgcccagatg ccccattgat
      721 agttccatgg gtatttgccg gaatgagaat tttcgttggg gtcattgttt atgtgaccgt
      781 tcgggtagta gagcaccgct ttattgttaa tgaaatgttc ccattcgcat tcagagccac
      841 cccggtcgaa atacgtattg accacgtgca gattattgcc cgtgtacgga agatgctgaa
      901 ccacgaagtt ggttgggagg agctgggcgt acagttcgcg ctggaccgcc cgatcgtagc
      961 tctgcaaaag caggcctgtg gcattgttgt cccagggaac ctgatcggca agcagcggcg
     1021 cggccagggt cttgagccgt ccccagtcgg tcatgacgcg atcgaattca cttccgagag
     1081 agacgagtgt ggccgagaac tgattcgcca attgattcgc taaatccccg gccgctattt
     1141 cctggttctc ctgcgcctgc aggggcgtac cggcggccga attggtatga gacgcggctt
     1201 gattggcaat tgcggtgccg acggtcagcg cgacctcggt gcccgccgcc acaatcccgc
     1261 cgccgggaat ggcattgagc aagctggacc caacaccgaa aacatcactc aatatgcctg
     1321 tccatgaggc ggcatgcgcc tgggtggtga ggccgacttg caggttatgc aaaatcttgt
     1381 cgccatcgtc ctgcaacagc agcgacacgt tggcctgctg atcctggtac aggccgatga
     1441 cattgctttg gaagaggcgc gccagcgtga cgtattggaa ctcggtggtg agctgctgca
     1501 ccatggcatc gaaatctgcg ggactggaat tggggattct cgaggagtcg tacgtcagat
     1561 gtcgcagttc cgccagccaa atggcaggtg aaatattcag attaacatag gctgaacgaa
     1621 tatcgacgca acacagctgc tggctgatcc aatcgtaggc ggccttcgat ccggccggat
     1681 caggtccggg gaacggccag ggcgtggggt tcagcaacga cgccgacaag atatccgcga
     1741 tcgtcgtctt gctcaggttg aggcggtgtc cgaacgaggc cggactgtaa tacccctggt
     1801 ggttcacccg caggaagcct tccatgtccg cgccatggcc cgcaccggta ggtggcggaa
     1861 acgcttccgt ctcacgcgcg atgatggagc tcatctcagc gcccacctga tctagacccg
     1921 tatccgtgct ttctcctccg acaccgtcga caaacgatcc aaccaaggta tagtcgtcat
     1981 acaggtatgg gtcggcattg ggattgttgg cgaggtagaa gacctgttgc gttcccccca
     2041 aatcccccat caattgtgag gcggtgtacc aattgacgcg ggccccacag agcccggtgc
     2101 tggtactgga ttggcagtta taatttgtat atccaaaccc ggcgatgaaa aagagatacc
     2161 cataggattt gtagccggtg atggcgtcat acagcctatt gacttcactc gcgtcaagct
     2221 tgctgggaaa ggtctggtgc cggacaaccg tcaaatcctt gctgtttaag atcagcagat
     2281 ggaacccgcc ttggctgccg ttcagtgagc ccgacgcatg gggtacaccg tccacggtaa
     2341 tggtattcat cactccatcg ggaccatcgg ggatcgtggc ggtcgaaaaa ctgaccaggc
     2401 ggtcgtttct cgcaaatgtg aaattatgat gattatcgcg aatgagaaca ccggcgatat
     2461 ccccgagttg tgatggctcc gcccccttgg gggcggtgaa cgacgtattg ggagagccga
     2521 gactgtcaca tgccgggccg ctgccataat tactgccact gcaggtcagg ctttcaaaaa
     2581 aagtaccggg tggtggtttc ggtggcccgg acgtgggtgg gaagggtact ttgtttgata
     2641 tggaagagaa cgcgatctgc ttcgagtccc cgttggcgta agcaagtctc gaggaaagac
     2701 ctagcccatt cagcatattc ttgaaggatt cgcaatcctt tggaaaatcc ttactataag
     2761 ccttagagca cggggtaaaa ccaatggttc caaggctttg gatgagtaga atgcatccgg
     2821 cagaaagaca atcttcaggc gtgtaaacta ttgattgata aagcggggag tcgtgccacc
     2881 ggccatcact gggaggccgc tgattaacga ccgaaacatt ggatatcacc gacaaatccc
     2941 agcgcctcat aaagactacc tggaaaccgg cattcgaacc gtcgctaggg acggacgccg
     3001 gtattagcgt tgctcccgtg ggatcgtcta gatccttgta gagcgcgatg ttgtagtccg
     3061 ctgccttgtc gcctgagccg gtaaccacgc gcgtcttgat ggggatcagc agcggcagat
     3121 tggccccggc aggactgctg tccggatgat caaccaggaa aaaatgactg tttacctcgt
     3181 gcgatcctga tctgactaaa atctgatttt tgccccaatt gaccaagggc cggtcaacct
     3241 cggcccggcg aacaccattg cggccaggcg ccataaaccg cgaggtgatg tcgacgccat
     3301 ccaacaatac ctgcatggcc tttgccgcat gatgtgcaac gcgcacctgg atggtgatgg
     3361 gtgccgtggt atgcgccgag gcattcgtcg gggacatgat catgattgat ttccgctgca
     3421 atgtatcgtt actggattcc gccagcaaag ttgagctact gatacatgcc tgcagcagaa
     3481 aacacaaaca cgtgagagaa aaggacaacc gacgattctt catatgatcc cccaatattg
     3541 atcagatgtc atgaatcggt atatttattt tgcgcgtatg cgcatcaaga actagccttc
     3601 agcactcgat cggaatcgta tcccgtggcc aacataacga aagaaacgaa cagaatcaac
     3661 aaatataggc cgttcctcgt cacgaataca gatgactgaa aggagaataa aaaaagacgg
     3721 tacgtgtgag gacagaaact tgatgacggc aagatcagcg tcaataattt cgaggttcat
     3781 ggagttccaa cgggcagcgc cattgaatgt gatgtcattc catgcgcgct cgccgggttt
     3841 ccgcttcgat g
//
LOCUS       DAORAQ010000238         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3353_length_3818_cov_56.658517, whole genome shotgun sequence.
ACCESSION   DAORAQ010000238 DAORAQ010000000
VERSION     DAORAQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3353_length_3818_cov_56.658517"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="PKM72_13425"
     CDS             <1..1470
                     /locus_tag="PKM72_13425"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNP61840.1"
                     /translation="LIPAIADALCWIVPRSVLQDQGARGFQDPSHRALLGHRLEAMMT
                     TNQENPTRGCAAYVTTYQALAADTRKINAKEFRRKRYILVLDEPHHLEEGGLWHEAIQ
                     PLYDRAALRVLMSGTFERGEGTPIAFLPYSTTDRGNRLDWDNTESRAVIHYTLADALR
                     EHACIDLTVHYANCQATWLDAQGAEHHVESLAHAGKQTAAALLTALKTEAALELLSTG
                     VKDWQVHRTTHSRSKLLVVAANIEQAQKYTAWLQERGVPARIATSEDSTAAYQAIKAF
                     KRQGSGAVDCLITVAMAYEGLDVPAITHLICLTHIRTKPWIEQVVHRATRMDPLAGPY
                     AEQRAYIYAPDDRPFRECLGYILAQRTTALADSPSSTQPLGEGTSPDAPGLLPTDRAE
                     SSIQPLRSGVLEMRNQGLWANLAAPGQMETNQETPKERLDRLRKKIEQHVRAHEQSKK
                     LSHGTVNRAVYSQFRKSRTAMTETELHKVMQWVHKIYPL"
     gene            1500..2357
                     /gene="ylqF"
                     /locus_tag="PKM72_13430"
     CDS             1500..2357
                     /gene="ylqF"
                     /locus_tag="PKM72_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNP61841.1"
                     /translation="MSIQWFPGHMNVARKEAAKAMEAIDVLVEILDARMPDASSNPLI
                     TELRLHRQRPCLKVLNKADLADPAVTQTWLNEFNREPDVNAVALSCNHAGQAAKIPGL
                     CQLLAPHRHSTSKPLRMLIMGIPNVGKSTLMNSLLKRRLAKVGNEPAVTKVQQRHTLN
                     DHMTLIDSPGLLWPKIEDPAVGLLLATIHAVTAKVMVEEEIAEFLVTILLARYPTLLT
                     KRYGFLTEGLDSMGVLEAIATKRGCLRKGKGGELDREKAAKILLTEFRNGTLGRISLE
                     TPETRDALL"
     gene            complement(3070..3429)
                     /locus_tag="PKM72_13435"
     CDS             complement(3070..3429)
                     /locus_tag="PKM72_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61842.1"
                     /translation="MGPTKAVVKDFAIYDAKTGKLIKDGFPSPEAIQDYAAHHYLVLP
                     VVNKDCQPWMLDGQPIFCLRGTRYENIKDEVLHLARCPDCGGMGIRADEPVVESDCIR
                     CVSCGHEFDTRLEMMES"
BASE COUNT          985 a         1093 c          966 g          774 t
ORIGIN      
        1 ctgattccag ccattgccga cgccctgtgc tggattgtcc ctcgcagtgt cttacaggac
       61 cagggtgcgc gcggttttca ggatcccagc caccgggcgc tcttagggca tcggctggag
      121 gccatgatga ccaccaacca ggaaaatccc acaagagggt gtgcggccta tgtcaccacc
      181 tatcaggccc tggcagccga cacgcgcaag atcaacgcca aagaattccg ccgcaagcga
      241 tacatcctcg ttctggatga accacaccat ctcgaagagg gtggcctgtg gcatgaggcc
      301 attcagccgc tctacgaccg ggcagcactg cgagtattga tgagcggaac gttcgaacgt
      361 ggtgaaggca cacccatcgc cttcctgccg tattccacca ccgaccgggg aaaccggctt
      421 gattgggaca atacagaatc ccgggccgtc attcattaca ccttagccga tgcgctgcgt
      481 gaacatgcct gcattgattt gaccgtccat tacgcgaact gtcaggcgac ctggttagac
      541 gctcagggag ccgagcacca tgttgaaagc ctggcacatg caggaaaaca gaccgcagca
      601 gcacttctga cggcgctgaa aaccgaagca gccctggaac tcttgagcac gggcgtgaag
      661 gattggcagg tccatcgcac gacccattcc cgatcgaagc tcctcgtagt ggcggccaac
      721 attgagcagg cgcaaaagta tacggcatgg cttcaggaac gaggcgtgcc ggccagaatt
      781 gccacgagcg aggattccac ggcagcctat caggcaatca aagcctttaa acgacagggt
      841 agcggagccg tggattgcct gattaccgtg gccatggcct atgaaggcct ggatgtgccg
      901 gccattaccc accttatctg tttaacccat atccggacca agccatggat cgaacaggtc
      961 gtgcatcgag ccacacgcat ggatccgctg gcgggacctt atgcagagca acgggcttat
     1021 atttatgctc cggacgaccg gccattccgt gaatgcttgg gatatatcct ggcccaacgg
     1081 accacggcgc ttgccgacag cccctcatcg acacagcctc tcggagaagg gacatccccc
     1141 gatgcaccgg gcctccttcc gaccgatcgg gctgaatcaa gcattcaacc cttgcggtcc
     1201 ggcgtcctcg aaatgcgcaa tcagggattg tgggcgaacc tggccgcacc gggtcaaatg
     1261 gaaaccaacc aggagacccc gaaagaacgg ttagatcgtt tgcggaaaaa aattgaacag
     1321 catgtccgtg cgcacgagca atcaaaaaaa ttgtcccatg gcacggtgaa ccgggcggtc
     1381 tattcgcagt ttcgaaaatc gcgcacagcc atgaccgaaa ccgaactcca taaagtcatg
     1441 caatgggtcc ataagattta tcccctctga ggtcattttt cccaggaagt ctcttacaca
     1501 tgtccataca atggtttccc ggccacatga atgtcgcgcg caaggaagcg gccaaagcga
     1561 tggaagccat cgatgtcctg gtcgaaatac tggatgcgcg tatgccggac gccagcagca
     1621 acccgttaat cacggaattg cggctgcacc gccagcgtcc ctgtttgaaa gtgctcaaca
     1681 aagccgacct cgccgacccc gccgtcactc aaacctggct gaacgaattc aaccgggaac
     1741 ctgacgtcaa cgccgtcgca ctctcctgta accatgccgg ccaggccgcc aaaataccag
     1801 gtctgtgtca acttcttgcc ccgcaccgtc acagcaccag caagccgctt cgcatgctga
     1861 tcatgggcat ccccaacgtc ggcaaatcca cgctcatgaa tagcctgctc aaacgccgcc
     1921 tcgccaaagt cggcaatgaa cctgccgtga ccaaagttca gcaacgccac accctgaacg
     1981 accacatgac gcttatcgac tcacccggcc tcttgtggcc aaaaatcgaa gatcctgccg
     2041 tcggactctt gctcgccacc atccacgcgg tcaccgccaa ggtgatggtc gaggaagaaa
     2101 tcgccgagtt tctggtcacc atccttctcg cgcgctaccc tactttgctg acgaaacgtt
     2161 acggcttcct cacagagggt ctggatagca tgggggtgct cgaagccatc gccacaaaac
     2221 gcggatgtct gcggaaaggc aagggcggag aattggatcg ggaaaaagcg gcaaaaattc
     2281 tcttgacgga attccgcaac ggcacgctgg gtcgcatcag cctggagaca ccggaaaccc
     2341 gagacgctct cctctaatgt cagacttccc caacggattt tctgcagacg gaatatgcct
     2401 cagtcgtaag gaggatcgat ctgaacacag atccttcgcc atgcctgccc tgagtccttc
     2461 gccttttcct caggataaac tccgtcgaag ggggctcagg aagaccaatg aatgcaagat
     2521 tgttttactg agaaattggc ccctcatgag ctcaatggtg gaagactgcc tttttcagaa
     2581 cctctaggtt ttttcgttcc cttcgagaca tttgatacat tcatccattt gcgggtcgcc
     2641 atgaaagcga caaaagtgcc gggaacattg tatgcaaacc aaatagctta gactttcaga
     2701 ccctgattcc ttacacaaat agcagggctt ttcccgtttc gcattgggca tggcttttct
     2761 cctggaaagg atgaggatga gggtatctcc agagattcga ggcgaccgct aatccactgc
     2821 tacgcccagg atcaggccaa cgctcgagac aagtcactat cggaagagaa tatggagaac
     2881 atggaaatag ggatgttcgg aaggaaagag catcatgaaa ggagtgtaaa gataaggacc
     2941 ccatgaagat ggagaaagac ggtatgaggc tagacggtct cctgtgaact tgaaggcagt
     3001 cgccaatcaa catagttgtt caaagcgaaa caatcgggaa gaatattcca ggttacagac
     3061 tacattacct caactttcca tcatctccaa gcgggtatcg aactcatggc cgcaggacac
     3121 gcaccggata caatcggatt caaccaccgg ttcgtcagcc cggataccca taccaccaca
     3181 atccggacat cgtgccagat gcaatacttc atccttgatg ttttcatagc gagtgccgcg
     3241 taaacaaaaa atgggttgac catcgagcat ccatggttga cagtctttat tcaccacagg
     3301 aagaaccagg taatgatggg ccgcatagtc ctgaatcgcc tcaggagaag ggaacccgtc
     3361 cttaatcagt ttcccggttt tggcatcata gatcgcgaag tcttttacga cagctttcgt
     3421 tggccccata agatacctcc ttgcgacagg atgaatctgt tcttcaacag tgagacaccg
     3481 ccaagtatga cctaacgatc tgataaaaga ttatgaagtc caagggccaa ggttcacaac
     3541 cacgaacatg ccatggaaaa acaaacaggc gcctgacggc gaacgaggcc ttcctctctt
     3601 acagaacaga aaagtcacgc attggccggc ccgattacaa aggatgtgga gaaaaggcca
     3661 ctatcacttc ttctttttgg atttgcccca ggtttcactc aggaccgatt tccactcatc
     3721 gcgggatacc gccggcattg ttttaatgcg aatgtgcccc attgaggcat tgagcacacc
     3781 ggccttcatg gccgcgcgat tattgggaaa ttctaaaa
//
LOCUS       DAORAQ010000239         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3356_length_3812_cov_69.574927, whole genome shotgun sequence.
ACCESSION   DAORAQ010000239 DAORAQ010000000
VERSION     DAORAQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3356_length_3812_cov_69.574927"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKM72_13440"
     CDS             <1..608
                     /locus_tag="PKM72_13440"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNP61843.1"
                     /translation="DKAYGDVEVLPYTEDMPPLGRFPYDVSKSCTDLLARSYSQTYDL
                     PVTIARCGNIFGGGDLNWSRIVPGTIRSFLRQECPIIRSDGKFTRDYIFVLDVVQAYL
                     LLALHAREEGVRGEAFNFSGEQPWTVLDVVREIQIQMECHQLAPIVMNQANAEIRDQY
                     LDSSKAKRMLKWAPLYSLEKGLAETITWYKEFLEQKSMTKC"
     gene            771..2009
                     /locus_tag="PKM72_13445"
     CDS             771..2009
                     /locus_tag="PKM72_13445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP61844.1"
                     /translation="MKNSQSCRFCRTVLEHTFLDLGTSPFSNSYVKAECLGEMEPFFP
                     LYAFVCSRCLLVQLKDCSTPERIFSDYAYFSSYSDLWLQHARAYTDMIQERIKLDERS
                     LVVEVASNDGYLLQYFVKKGIPVLGIEPAENVAKVATEKGIPTRVSFFGERSAQILVE
                     EGKQADLLIGNNVLAHVPDLNDFVRGLQTLLKPQGVITMEFPHVMRLMDENQFDTIYH
                     EHFSYFSFGTVEQVFRKHGLILFDVEELPTHGGSLRIYARHEADHSKPVESRVAALQD
                     REKKAGLQTLDHYLSFAEKVHHTKRQLLSFLIEAKEKGKTIVGYGAPAKGNTLLNYCG
                     IRTDFIDYTVDRSPHKQGLYLPGTHIPIHDPDSIRRTKPDYVLILPWNLKDEIMQQMA
                     CIREWGGAFVVPIPEVRVYP"
     gene            1958..2545
                     /gene="rfbC"
                     /locus_tag="PKM72_13450"
     CDS             1958..2545
                     /gene="rfbC"
                     /locus_tag="PKM72_13450"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNP61845.1"
                     /translation="MGRGIRGADSGSPGVSVIFTESTIKGAFLIDPEPIEDSRGFFAR
                     TVCQREFEDHGITFRQVQCNLSFNKKKGILRGMHYQCAPYEEAKLVSCIKGAIYDVLI
                     DLRPLSQTYTQHAAVVLTAENRRMHYIPEGCAHGFQTLEENTEIFYQISAFYDPRCAK
                     GVRWDDPAFGIQWPADERLISDRDLSYPDFIEPQH"
     gene            2569..>3812
                     /locus_tag="PKM72_13455"
     CDS             2569..>3812
                     /locus_tag="PKM72_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNP61846.1"
                     /translation="MDNKVSNQIGHEIYQFLAELYPICRSITGHGVRETLQGIARHVP
                     VKMHEIPSGTEVFDWVVPQEWNIQDAYIKNKKGERIVDFKQSNLHVVSYSQPIHTTMT
                     LEELKPHLYSLPENPDWIPYRTSYYKESWGFCLTHRQLMAMKDEEYEICINSTLESGH
                     LTYGEYIIKGETEEEILISCHTCHPSLCNDNLSGIAVTTFLAKTLSGRSLRYSYRFLF
                     IPGTIGSITWLALHETQSSTITNGMVVTGVGDPGPLTYKKSRQGNAGIDRAFTHILKT
                     SGLEHAIIDFFPYGYDERQYCSPGFNLPVGCFMRTPHGEYPEYHTSGDNLTFVQPQSL
                     EESLICCERALNILERNKTFLSQNPKCEPRLGKRGLYRAIGGQSEGAKREMAMLWVLN
                     FSDGMHSLLDIAERSGLEFESI"
BASE COUNT         1005 a          841 c          963 g         1003 t
ORIGIN      
        1 gtgataaagc ctatggtgat gttgaggtct tgccttatac ggaagatatg ccgcctttgg
       61 gacggtttcc ctatgatgtg agtaaaagtt gtaccgattt gctcgcgcgc tcgtatagcc
      121 agacatatga tttgcctgtc acaattgcaa ggtgtggaaa catttttgga ggaggcgatc
      181 taaattggag tcgcatcgtt ccgggcacga ttcgaagctt cctgaggcag gaatgcccca
      241 tcattcggag cgatggaaaa tttacacggg attacatctt cgtccttgat gttgtgcaag
      301 cctatcttct tctggcctta cacgcacggg aggagggagt tcgtggcgag gcgtttaatt
      361 ttagcggaga acagccgtgg acggtcttgg atgttgtgcg cgaaattcaa atacagatgg
      421 agtgccatca gttagcccca atcgtaatga accaggccaa tgccgagatc cgggatcaat
      481 atctcgactc cagcaaagct aaacggatgc tcaagtgggc tcccttgtat agtttggaaa
      541 aagggctggc tgagacaatt acatggtata aagaatttct cgagcaaaaa agtatgacga
      601 agtgttaaag atgaaggccc ttcacattgg gccctgttaa aaatattcct cccaatacac
      661 aatacgtgac tggtaaatcg tcgaaatgtt tcgtttttgg cgtggattgc agccggcccc
      721 ataatccccg atttccggag tgaaggcggt cgactcaaga ggggaaaact atgaaaaatt
      781 cacagtcttg tcggttttgc cgtacggtac tagagcatac gtttcttgat ctagggactt
      841 cccctttttc aaattcctat gtgaaagccg aatgcttggg ggagatggaa ccgtttttcc
      901 cattgtatgc ctttgtctgt agccggtgtc ttctggtgca gttaaaggac tgttcaactc
      961 ctgagcgaat ctttagcgat tatgcctatt tttcttccta ttctgatttg tggcttcaac
     1021 atgcgcgcgc ctatacagat atgattcagg aacggataaa actggatgaa cggagtcttg
     1081 tggttgaagt cgccagtaat gacggctact tacttcaata tttcgtcaaa aagggcattc
     1141 ccgttttggg aatcgaaccg gcggaaaatg tggccaaagt ggctaccgaa aaagggatac
     1201 ctacacgggt atcgtttttt ggggaacgtt cggctcaaat tttggttgaa gaaggaaagc
     1261 aggcagattt gttgattggc aataacgtct tagctcacgt cccggacttg aatgattttg
     1321 tccgtgggct tcaaacgctg ctcaaacctc agggtgtcat caccatggaa tttccccatg
     1381 tgatgcgcct catggatgaa aatcaatttg acacgattta ccacgaacat ttctcgtatt
     1441 tctcatttgg caccgtagaa caggtattcc ggaagcacgg gttaattctt ttcgatgtcg
     1501 aagagctccc aacccatggc ggatctcttc gaatctatgc tcgccatgaa gccgatcact
     1561 caaagccagt ggagtccaga gttgcggcct tgcaggatcg ggagaaaaag gcaggcttac
     1621 aaaccttaga tcattatctt tcttttgccg aaaaagtcca tcataccaag aggcaactcc
     1681 tgtccttctt gattgaggcg aaggaaaagg ggaagactat cgtagggtat ggtgctccgg
     1741 ccaaagggaa taccctcttg aattattgtg ggattcgtac ggatttcatt gattacaccg
     1801 ttgatcgtag ccctcacaag caagggcttt acctgccagg gacgcatatt ccaatccatg
     1861 accccgattc gatcaggaga acgaagcctg attacgtctt gatccttccc tggaatttga
     1921 aagatgaaat tatgcagcaa atggcctgta tccgcgaatg gggcggggca ttcgtggtgc
     1981 cgattccgga agtccgggtg tatccgtgat tttcaccgaa tcaacaatca agggagcttt
     2041 cttaattgat ccggagccta ttgaagacag tcgggggttc tttgcccgaa cagtgtgtca
     2101 acgagagttt gaggatcatg gaatcacatt cagacaggta caatgcaacc tttcttttaa
     2161 taaaaagaaa gggatccttc gggggatgca ctaccaatgt gctccgtatg aagaagccaa
     2221 gctcgtctcg tgcatcaagg gcgcaatcta cgatgtgctg atcgatctgc ggcctttatc
     2281 tcaaacgtat actcagcatg cggcggtcgt tctcaccgcg gaaaaccgac ggatgcatta
     2341 tatccctgaa ggatgtgccc atggatttca gactcttgaa gaaaatacgg agatttttta
     2401 ccaaatatcg gcgttctacg atcctcgatg tgccaaaggg gtgcggtggg acgatcctgc
     2461 gtttgggatt caatggccag ccgatgaacg cctcatatct gaccgcgacc taagttaccc
     2521 cgattttatc gaaccccaac attgaacacg ataaggctgc gtggccgtat ggataacaaa
     2581 gtttcaaatc aaatcgggca tgagatctat cagtttcttg ctgaactgta tcccatctgt
     2641 cggagtatta caggtcatgg cgttcgggag acactacaag gaattgcacg ccatgtcccc
     2701 gtgaagatgc atgagattcc aagcggaacc gaggtgtttg attgggtagt gccacaggag
     2761 tggaacattc aggatgcgta tattaaaaat aaaaagggag aacggattgt cgattttaag
     2821 caatcgaacc tgcatgttgt cagttatagt cagcccattc acaccacgat gacgcttgaa
     2881 gaattaaaac cccatctgta ttcattgccc gagaatcctg attggattcc ataccgaacc
     2941 tcctactaca aggaaagctg gggattctgt ctgactcatc ggcaattgat ggcgatgaaa
     3001 gatgaggaat atgagatatg tatcaattca accttagaat ccggacattt aacctacggg
     3061 gaatacatca taaaagggga aactgaagaa gagatcttga tctcctgtca cacctgccat
     3121 ccatccttgt gcaatgacaa cctgtccgga attgccgtta cgacgtttct ggccaagacg
     3181 ttgagcgggc gctcattacg gtattcttac cgctttctct ttattccagg gacgatcggg
     3241 tctattacct ggctggcgtt gcatgaaacc caatcgtcca caatcaccaa tggcatggtg
     3301 gtgaccgggg tgggagatcc cggaccatta acgtataaaa agagccggca aggcaatgcc
     3361 gggattgata gggccttcac gcatatcctg aaaacctcgg ggttggagca tgccatcatc
     3421 gatttcttcc cctatgggta tgatgaaagg caatattgtt ccccgggctt caatttgcca
     3481 gtgggctgtt tcatgaggac acctcacgga gaatatcccg aatatcatac gtcaggcgac
     3541 aatttaacgt ttgtccagcc tcagtcgttg gaagagtcct tgatctgctg tgaaagggcg
     3601 ttaaatattt tggaaaggaa caaaacattt ctcagtcaaa atcccaagtg cgaaccccgt
     3661 ttaggaaaaa ggggcctcta ccgggccatt ggtggtcaat cggaaggcgc gaaaagggag
     3721 atggcgatgt tatgggtatt aaatttttcg gatggaatgc attcgctctt agacattgcc
     3781 gaacgatctg gattggagtt tgagtccatt ga
//
LOCUS       DAORAQ010000240         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3375_length_3798_cov_72.420251, whole genome shotgun sequence.
ACCESSION   DAORAQ010000240 DAORAQ010000000
VERSION     DAORAQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_3798_cov_72.420251"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKM72_13460"
     CDS             <1..335
                     /locus_tag="PKM72_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61847.1"
                     /translation="HDQNLPALVSAGSRVQFEHGRLSRPFSVDLSTVSAWLNGRLIVN
                     GVPLEQVIDEVRRYYPGTILLLNQPIRDMHVTGTYNLEDPSKSLSLLAKTFPTHMFNL
                     ANRFVILF"
     gene            432..3020
                     /locus_tag="PKM72_13465"
     CDS             432..3020
                     /locus_tag="PKM72_13465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNP61848.1"
                     /translation="MRSDNQSIVEPYLRQHANRSHPKTWLGRVLVVMLLIVMHLSPAM
                     VQAVLAETGPTQIVNFDIPSQPVGSALNAFADATGWQISLPTELAAGLTSPGVIGSYT
                     PEVALQTLLSGTGLTYQLADTNAVILVQQSSTGAVPSALPSQRPVVSRESPPSSSKAT
                     PKTVKVPEIVVKEVRERDDAKTYVAEESHTATRTDTPIRDVPQSIQVITRKVIEEQRT
                     YRLQNTLENISGINATESTASLYDSLIIRGFAATDRSYYRNGLLDPFAQFMASDTYNI
                     RRIEVLKGPASVLYGQGDPGGIINLVTNKALPNAEYSASTTLGNYNFYRSDLNATGPL
                     NKNKTLLYRLNVAGQKANSFIDYANRDLVAVAPSLTWLIGSRTSFTVDFDYMRRWTND
                     PYGLPAQGTYLPNPNGPISRKTSTTLGSFSTFNRSSYRVGYDLSHQFNDKWSVRNAYR
                     YSIVEDDKDNLYAGFDSLDPDQRTIQRFQVLQPVVARRHAHSMVTNVVGHLQFLNMEH
                     TLLTGVELRQEKTDQFTFAFGEAPPLDLYNPDYSLRPNPFDGPRISNVGDTKSVGVYM
                     QDQVTILPNLKFLGGVRYDYVDQYQKFTFAGDSTKDSLDNDAWSPRLGLVYQPIEPVS
                     LYTSWTKGFQPSSASSVNPDGESFKPERSTQYEVGIKAFLLDNRISATLAWFHLTRKN
                     LLTPDSNPAQAAQGFMVQTGEQRSQGVEFDVTAQLTAGWNVIAGYAYTDAEVTKDNDP
                     TLEHKRLANVPYNKFTLWSTYHFQEGVLKGFGVGGGIFAYTDRNATIFENMPTMPGYV
                     RADAALYYNRELPPGNWLGAKGVNAALNFRNLLDQRYVATSYNGSSSFFFGEPLTVLG
                     TLGIRF"
     gene            3124..3384
                     /locus_tag="PKM72_13470"
     CDS             3124..3384
                     /locus_tag="PKM72_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61849.1"
                     /translation="MKIAFLMVVLALTACAEGRWVHPTKTSVEETQEDWDTCKAEVLS
                     GKEHGKSSLAGGINLSGCMQSKGYRYEVPPPPNSSVDIPTNH"
BASE COUNT          941 a         1040 c          933 g          884 t
ORIGIN      
        1 tgcatgatca aaatttgccc gcgctcgtat ccgccggatc ccgggttcaa tttgaacacg
       61 gtcgattaag tcgtcctttc tccgttgatc tctcgacggt atcggcctgg ttaaacggac
      121 ggctcatcgt caatggagtc ccgctggaac aggtgattga cgaggtacgc cggtattatc
      181 caggcacgat cctgcttttg aatcaaccca tccgcgacat gcatgtcaca ggtacataca
      241 atcttgaaga tccctccaag agcctctccc tccttgccaa gacctttcca acccacatgt
      301 tcaatcttgc caaccggttc gtcatcctct tttaaaaaaa atcgttttcc tcacaatctt
      361 tggcagaaga agtgaggaaa tttttgtggg cttccgtcta aacagacata cgaacaacca
      421 ggaggcgccg catgcgatca gacaatcaat cgatagttga accttatctg cgacaacacg
      481 cgaatcggtc acatccgaag acatggcttg ggagagtgct tgtcgtgatg ctgctcatag
      541 tcatgcattt gtcacccgcc atggttcaag cagtcctcgc cgagacaggg ccaacgcaga
      601 tagtcaattt cgacattccc tctcaaccgg tcgggtcggc gttgaatgcc tttgccgatg
      661 cgaccggttg gcagattagt ttgccgacgg aactcgccgc cgggctgacg tcgccaggag
      721 tcattggcag ttacaccccg gaagtggcgt tgcaaacgtt gctctcagga acaggattga
      781 cctatcaact tgcagatacc aacgctgtca tcctggttca gcaatcatca acgggagctg
      841 ttccctcagc cctcccatcg caacgtccgg tggtctcgag ggaatcccca ccttcaagca
      901 gtaaggccac acccaaaacg gtgaaggtgc ctgagatcgt ggtgaaagag gtgcgagagc
      961 gggacgacgc aaaaacctat gtggccgaag agtcccatac ggccacccga acggatacgc
     1021 cgattcgtga tgtgccgcag tcgatccagg tcatcaccag gaaggtgatt gaagaacagc
     1081 gaacctaccg cctgcaaaac acgctggaaa atatttcagg catcaatgcg acggagtcca
     1141 cagcctccct ctacgattca ctcatcattc gaggattcgc tgccaccgac cggtcctatt
     1201 atcgcaatgg cttgctcgac cccttcgctc agtttatggc gtcggacaca tataatatcc
     1261 ggcgaatcga agtactgaaa gggccggcat cggtgctgta cggacaggga gatccaggcg
     1321 gcatcattaa tctcgtcacc aacaaagctt taccgaatgc ggagtattcc gccagcacca
     1381 cgttggggaa ttataacttt tatcgatctg atctcaacgc cacgggtccg ctcaataaga
     1441 ataagacact gctctatcgt ctgaatgtgg cgggccaaaa ggcgaatagc ttcattgatt
     1501 atgccaaccg cgatcttgtg gcagtcgctc caagcctgac ctggcttata ggttcacgga
     1561 cctccttcac ggtcgatttc gattatatgc ggcgttggac caacgacccc tacggacttc
     1621 cggcccaggg cacctacctc ccaaatccta acggccccat atctcgaaag acctcgacga
     1681 ctctcggcag cttcagcacg tttaaccgta gctcctaccg ggttggttac gatctctccc
     1741 atcagttcaa tgacaaatgg tccgttcgca atgcgtacag atattcgatc gtggaagatg
     1801 acaaagacaa cttgtacgcc ggttttgatt ctttggaccc tgaccaacga accatccaaa
     1861 gatttcaggt gctgcagccg gtggtggccc gccggcatgc ccactcgatg gtgacgaatg
     1921 tggtgggaca tctgcagttc ctgaatatgg agcataccct attgaccggc gtcgaactca
     1981 ggcaggaaaa aaccgatcag ttcacctttg ccttcgggga agcgccaccg ttggatctct
     2041 ataatcccga ctattccctg cgcccgaacc cgtttgacgg acccaggatc agcaatgtgg
     2101 gtgacacaaa aagcgtcggg gtctatatgc aagaccaggt gaccattctc ccgaatttaa
     2161 aattcctggg tggcgtgcga tacgactatg tcgaccagta ccaaaagttt acctttgcag
     2221 gagactccac aaaggattcc ttggataacg atgcctggag tcctcggtta gggttagtgt
     2281 atcaaccgat cgaaccagtg tccctctata cctcctggac gaaaggtttt cagccaagct
     2341 cagcctcgtc ggttaatcct gatggtgaat ctttcaagcc ggaacggtcc acccaatatg
     2401 aagtggggat caaagccttc ctgttggata atcgtatttc cgccacgctc gcctggttcc
     2461 acctgactcg aaagaatctg ctgacccctg attctaatcc tgcgcaggca gcccaagggt
     2521 tcatggtaca aacgggcgag caacgcagtc agggagttga atttgatgtc actgcccaac
     2581 tgacggctgg ctggaacgtg atcgccggct atgcctatac cgatgccgag gtgacaaaag
     2641 acaacgatcc gacattagaa cataagcgcc tggccaatgt gccgtacaat aaatttactc
     2701 tttggtccac gtaccatttt caggaagggg tcctcaaagg attcggagtt ggcggcggca
     2761 ttttcgcata caccgatcgc aatgccacaa tttttgaaaa tatgcccacc atgcccggct
     2821 atgtcagggc cgacgccgcc ttgtactaca atcgtgaact gccaccggga aactggttgg
     2881 gagcgaaggg agtgaatgca gcgcttaact tccgcaacct gctcgatcag cgatacgtcg
     2941 ccacgtcata taacggttca tcaagtttct tcttcggcga gccgcttaca gtgctgggaa
     3001 ccttgggaat acgattctag gagtgccaga caaggagaga acgaaaacac ccagtcagtt
     3061 ggagaagttg ccaaccggat cacggctttc ctcaagagaa agtccattac gaaggagtca
     3121 catatgaaaa tcgcattcct catggtggtg cttgcgctca ctgcctgcgc cgaaggccgg
     3181 tgggtgcatc cgaccaagac ctcggtggaa gaaacccagg aagattggga tacttgcaag
     3241 gccgaagtgc tgtcgggaaa ggagcatggg aaaagttcgt tggcgggcgg catcaatctg
     3301 agtggatgca tgcaatcaaa agggtatcgc tacgaagtcc caccgccgcc taattccagc
     3361 gtggatattc ccacgaacca ctgacgctct ccttttcact ccgactggaa taaaatgtgg
     3421 gaatttgtac tattttgaga ggagcttcaa tttttggtct ttgattctcc ctccaagtac
     3481 cctcctcatg tttaacttga tcatcggatt tttgagtgaa ccttagggct aggccctttc
     3541 ccctcttgtg cgttttccct ccatttgata gacaagactc ctgtttgtgt ggccctaaaa
     3601 aattcccatc tgaaagattt gaacccgcca agatgacgga accataaaat caccaaaaac
     3661 gaccgacacc atgaaggcca taacctggtt cattaaaaat cctgttgcag ctaacgtcct
     3721 catggggctt attgtcctgt ccgggctcgt cggccttttc acggtagaga aagaagtgtt
     3781 tccccgtttc agtcagca
//
LOCUS       DAORAQ010000241         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3392_length_3778_cov_57.992748, whole genome shotgun sequence.
ACCESSION   DAORAQ010000241 DAORAQ010000000
VERSION     DAORAQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_3778_cov_57.992748"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..287
                     /locus_tag="PKM72_13475"
     CDS             156..287
                     /locus_tag="PKM72_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61850.1"
                     /translation="MDRDAVSERENEKSKRPNAGMGIVGGVVGLKKKDNPRQSVICT"
     gene            complement(284..1075)
                     /locus_tag="PKM72_13480"
     CDS             complement(284..1075)
                     /locus_tag="PKM72_13480"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF014199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNP61851.1"
                     /translation="MNIRVLGAHGSDLLLNGPTSHHICRSVGFLVNEELMVDAGTLAS
                     GLTLEEQKRITHILLSHLHLDHVKGIPPLVDNMSGRVDHQIVVASLPSVLVGLQKHVF
                     NDLVFPNFFHLPESQYSMLRGEELEPKREWRLPGRIRVTPIPVNHTVETAGFIIQDDE
                     VAWIYSGDTYVTENLWQEAARIPNLKGVFIEVSFPDSMKDMASRSKHLTPTLLAHEYR
                     KIGKPDLPIYVYHMKPTVRDQIIDEIIHLDIPHVTILEEGQELKF"
     gene            complement(1362..2030)
                     /locus_tag="PKM72_13485"
     CDS             complement(1362..2030)
                     /locus_tag="PKM72_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNP61852.1"
                     /translation="MVMYKQAIKNVPLFSELTDQELNLLAASGSRQKFPDKNVIFQEG
                     DSGEVLFIILSGKVKVLLTGKNGQEFILSHLGPGNFFGEMAILESAPRSASVITAEPS
                     EFFLLGQKELKELLTNHPDIVIKILKNLSQRLRKVSEQVRSLVMFDMYGRVGRCLLDL
                     AEFQDGVETHGQLLISNRPSFQELANMVGCSRETLSRTLKALKENGSLTVTRNTIYIN
                     RLWE"
     gene            2986..3489
                     /locus_tag="PKM72_13490"
     CDS             2986..3489
                     /locus_tag="PKM72_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61853.1"
                     /translation="MDSLPGLQNLETTVEPLRQQPGTLVDALMDVPTHRPESLPHFAS
                     RFTRPEGLQPENKFEPISLLTIPSPVHPGQTGHIDFTLINDDPTETAEHTLYTTNLVG
                     MSGHRIPETSIAVLPNPVRIPPGESVDGRIEIQAPTGTSPGSYSGLLQTEDITRPQAV
                     IRLSVCS"
BASE COUNT         1009 a          927 c          827 g         1015 t
ORIGIN      
        1 gtatccattt ggatacataa tttatctatt tagatacatg tcttggttga cgactgtttg
       61 tctttgaatc tcccaaggtc aatcattgcg atctctgtat ggtcttttag tccgaatgct
      121 ccatggtccc cctcaaatta ctcaggacag gactgatgga tcgagatgct gtttctgaga
      181 gagagaatga aaaatccaaa agacccaacg caggcatggg gattgttgga ggcgtggtcg
      241 gtttgaagaa aaaggacaat ccacgtcaat cggttatctg cacctaaaat ttcagttcct
      301 ggccttcttc caatatggtg acgtgaggga tgtccagatg gattatttca tcaataattt
      361 ggtctcgtac ggtaggcttc atgtggtaga cgtatatggg cagatcaggt ttcccgattt
      421 ttcgatattc gtgggctaaa agagttggcg tgagatgttt acttcgagag gccatatctt
      481 tcattgaatc gggaaaggat acctcaataa agacgccttt taaatttggt attcgggcgg
      541 cctcttgcca aagattttcc gtcacatagg tatctccact ataaatccag gccacctcgt
      601 catcctgaat aataaaccct gcggtttcga cggtatggtt aacgggaatg ggggttactc
      661 taattcttcc aggcaaacgc cattcccgtt tgggctctaa ctcctcgcca cgtaacatgg
      721 aatattgaga ttctggaaga tggaagaaat tcggaaatac taaatcgtta aagacatgct
      781 tctgaagtcc aactaaaacc gagggtaaac ttgctaccac gatttgatga tccacccgtc
      841 ctgacatgtt atcgacaagt gggggaatgc cttttacgtg atctaaatgc aagtgggaaa
      901 ggagaatatg ggtgatccgc ttttgctcct ctaaagtgag tcctgatgct aatgttccag
      961 catctaccat cagttcttca ttaaccaaga accctacgct ccgacaaata tggtgactgg
     1021 tcggtccatt caagagcaga tcagacccat gggctcccaa tacccgaatg ttcacaaaaa
     1081 atcccttgaa tgacagcaat gatccatacc cttgccttcc tattgtgcca tattcaaaaa
     1141 atgcagggat gagcgaccaa tttcaagtcg tggaatccta gggggtaacc acctttccga
     1201 tggaaagaca atctgaggat cttttctagt ttttaattta ttccattcca gacctcctgt
     1261 ctgtgatatt catcacattc tttgtgtgat tctcctcaca ctccggccac gccgtatttc
     1321 cataaaatct atcaatagca ctaaaccaaa cttgttgggt tttattccca caacctattg
     1381 atatagatcg tgtttctggt gactgtaaga cttccgtttt ctttgagagc ctttagggtc
     1441 cgactcaagg tttccctgga gcatcctacc atgttggcta attcctggaa ggatggacga
     1501 ttggaaatca ataattgtcc atgagtctcc accccatcct ggaactctgc taaatcaaga
     1561 agacagcgac ccacacgccc atacatatca aacatcacga gactgcgaac ttgttcgctg
     1621 acttttcgca atcgctggga aagattcttg agaattttta tgacaatgtc tggatggttt
     1681 gttaacagtt ccttcaattc cttttgtccc aataaaaaaa attcactcgg ctccgctgtt
     1741 atcactgatg cggatcgagg agccgactcc agaatggcca tctcaccgaa aaagtttcct
     1801 ggacctaagt gcgagaggat aaattcctgc ccgtttttcc cggttaacag cacctttact
     1861 ttgcctgaca aaataatgaa caacacttcc cctgaatcgc cttcttgaaa tatcacattt
     1921 ttgtcaggga acttttgacg actcccggaa gcggccaaca gattgagttc ctgatccgtt
     1981 aattcggaaa agagtgggac atttttaatc gcttgtttgt acatcaccat gacaagaaac
     2041 ttcctgacga gtcattcttc ccttggattg agcagggatt agctctactt cgaaaaaaaa
     2101 agcacaataa ttctggcatc agcccatttc aagatttcaa atggagccat catgcaaaaa
     2161 taattcgata aagacaacac ccttgtgcat ggcagccact atccactgag cctccaggga
     2221 tacacgcttg ctcgatcaaa ctggtgacta ttgccgggac catcgtcagg ttggccaaat
     2281 tttcctcatg atttcaatgc atttcttctc atgtctcttt tgtctgatga tgagacacca
     2341 tccacaaaaa cctcctcgaa gagtccagta atccccaaac gtgtcccacc atgtcatgcc
     2401 tgggcttcct ggttcaccag cgccggcaag aacccgtttc cttaaaccta ttggatttgg
     2461 ttttgaataa ttctaggttt ccccttccag atttatcgct attgatatat ttgcgttctt
     2521 tagctagcct aaaaaaaatc ggatgcgctt tttgatcatc caatcctatt gaagttgatt
     2581 atttcggttt ttgggaatgg atgagctcag tgaagtattg atagaggaat gaacgaaaac
     2641 gactattctc cctgacttcg tatttgtatt cgatgtaacc agagcgatga ccatgagtag
     2701 attggatgtt tcagcaaata tcagcattga ttagcttaat ttttcacgtt ctcatttatc
     2761 gttatggtac ttcccctggg cagaccatca acgacttcag gctctaggca cacaagggta
     2821 gatagtgcca tcattccagc agggtcagtt tctcatacac cccgccagcc ggaaactgca
     2881 ccgggcaatt ccacgaaacc gccgtcgaat ctaattcgaa aagcccacgc cgttcttcaa
     2941 aacgaactcg aatcacgtag aagtctggga acggaggtcc tgccaatgga ttccctccct
     3001 ggcttgcaga atctcgaaac cacggtggag ccattacgcc aacagccagg gacgttggtc
     3061 gacgctttga tggatgtgcc gacacatcgg ccagagtcct tgccccactt tgcatcccga
     3121 ttcaccaggc ctgaagggct ccagcctgag aataaattcg aaccaatatc cttgctcaca
     3181 attccctcac ctgtccatcc agggcaaacc ggtcacatag acttcaccct gatcaatgac
     3241 gaccccacag aaacagccga gcataccctg tatacgacga atcttgttgg catgtccggg
     3301 cacagaattc cagagacatc catagccgtg cttccgaatc cggtacgaat cccccctggg
     3361 gaatccgtag acgggcgcat tgagattcaa gcccccaccg gcacttcccc agggagctat
     3421 agcgggttgc tacagacgga agacattacc cggccccaag ccgtgatccg cctttctgtt
     3481 tgttcctaag aaagattcag gctctggaca ggattgcggt gatccaggat tctcaacaca
     3541 cgtgcatgga ccagtgcgtg gcgaaaaggg aaatggaact ggacggtcac gactgcggta
     3601 tgaccaatcg tgagagcggg gtgaaactaa gcctccggaa agtatgggtc ggtgcatgag
     3661 gagcccaagc ggccaagaag gccgcgggca atcttcttaa gcattctgtt cagagaggcg
     3721 agtcatgacg ttcgacgaga tcctggacgc gacaatgcga ttgcttgaga gccgaaag
//
LOCUS       DAORAQ010000242         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3400_length_3766_cov_78.036917, whole genome shotgun sequence.
ACCESSION   DAORAQ010000242 DAORAQ010000000
VERSION     DAORAQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_3766_cov_78.036917"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="sucC"
                     /locus_tag="PKM72_13495"
     CDS             complement(<1..1046)
                     /gene="sucC"
                     /locus_tag="PKM72_13495"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNP61854.1"
                     /translation="MNIHEFQAKQLFAQFGIPVPKGKEIKNVKAAEKWASVLDTPVFV
                     VKAQIHAGGRGKAGGVKLTKNKAEVPELAKEILGKTLVTHQTGPKGRKVRRLLIEEGA
                     GIAKELYLSLLVDRESGFPTFIASTEGGMDIEEVAEHTPDKLLKESIDPAVGFQSYNG
                     RNLAFGLGLPELEPAVVKPFFQMLENLYRLFMEKNASLVEINPLVVTTDKRLVALDGK
                     VSFDDSGLFKHPDVQKFRDLHEEEPLEIEAGNNNLNYVKLDGDIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKETVAAGFRILLKDKKVKGIFINIFGGIVRCERIAHG
                     VIDAAKEVGIKLPVV"
     gene            complement(1130..2707)
                     /locus_tag="PKM72_13500"
     CDS             complement(1130..2707)
                     /locus_tag="PKM72_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNP61855.1"
                     /translation="MELTDQRNLVHQTRDVRRSKSFPKLSPAERDGLIKKYHPDHRAH
                     AYRPIIFGPNAGESTVTEVATLLEGESPVPADLNLTPDYSVDVLVVGGGGAGCASALH
                     AHAQGANVLLATKLRLGDSNSVMAQGGMQIAVGSDDSPVQHFLDTLKGGHMKNDHQLL
                     KVMVEEGPSIAKWLLELGVLFDRDADGNLHVKKGGGSSKARLLTCSDYTGLEIMRVLK
                     DEVLNQKVPLLEFVAAVELLSDAKGACTGAVLKDLDNNRYIVVAAKTVILATGGIGRL
                     HIQGFPTSNHFGATGDALVLAYRLGVPLVQIDTFQYHPSGGVYPEQLVGALVTEGIRS
                     EGGHLVNGKGERFVNELDTRDVVSSSIIRECEEGRGVRTPSGRVGVWLDTPLLDVEHG
                     PGTLDKHFPAMVRQYERYQVDIRKDPVLIYPTLHYQNGGVKIDVNGETPVANLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRTGTLAAERSKSLPKCSLTLDHLKRFRAEAKRHH
                     VSAGIISPMVLPAYTNK"
     gene            complement(2729..3445)
                     /locus_tag="PKM72_13505"
     CDS             complement(2729..3445)
                     /locus_tag="PKM72_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNP61856.1"
                     /translation="MPDTLDKKNTLDQEENLQPKMVTVEIEGKPFQVPAGITLIKALW
                     YAGQDVIRGVGCLGGFCGACATYYRTKDDPKVKTCLACSMAVEDGMSFSFMPAFPARK
                     ATYNLLELKDPKQDLFTLYPEAPLCRNCNACTEACPQHIDVREGVWKAVFGDFKAVSE
                     MFMDCVMCGMCAPVCIADIAPNLVALYASRAQGVHFTEKPEGLATRIQEITDGRFQQE
                     WDRILKLSDEELQNTTAATT"
     gene            complement(3529..>3766)
                     /locus_tag="PKM72_13510"
     CDS             complement(3529..>3766)
                     /locus_tag="PKM72_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNP61857.1"
                     /translation="LKKQGILALTFADPKDYDKIEQEDRISVKGLSGLAPGKPVQVVL
                     HKKDGQEVVIQANHSMTEQQLTWFKAGSALNALN"
BASE COUNT          908 a         1082 c          829 g          947 t
ORIGIN      
        1 acaacaggta gcttgatgcc gacttctttc gccgcatcaa tcactccatg ggcaatacgc
       61 tcgcaacgca caatcccgcc gaaaatatta atgaatatgc ctttgacctt tttatctttc
      121 aagagaatgc gaaacccggc tgcaaccgtc tctttcgtcg ccccaccgcc cacatccaaa
      181 aaattcgccg gctctgaacc cgccaacttg atgacatcca tggtggccat tgccaaaccc
      241 gccccgttga ccatacagcc gatgtcccca tcaagtttga cgtagttcaa attattattg
      301 cccgcttcga tctccaacgg ttcttcttcg tgtaaatctc tgaacttttg aacatcggga
      361 tgcttaaaca gcccgctgtc atcaaaggag acttttccat ccaacgcgac tagacgctta
      421 tccgtggtga ccaccagtgg attaatttcc acaagcgatg catttttttc catgaacagg
      481 cgatacaggt tttccaacat ttgaaaaaac ggcttcacca cagccggctc aagctctggt
      541 agacctaagc caaatgccag atttcttcca ttgtacgact ggaatcccac cgccggatca
      601 atggactctt tcagcaattt gtccggtgta tgttcggcta cttcctcaat gtccatgcca
      661 ccttcggtac tggcaataaa cgtcggaaat cctgattcac ggtcaaccag taaactcaaa
      721 tataattcct tagcaatccc tgcaccttct tcaatgagga ggcgccgcac ttttcgcccc
      781 ttggggccgg tctgatgcgt aactaaggtt ttgccgagaa tttccttcgc cagctcaggc
      841 acttccgctt tatttttggt caatttgact ccgccggctt tcccacgtcc accggcatgg
      901 atctgcgctt tcacgacaaa cacgggggta tcaagaactg aggcccattt ttcagccgct
      961 ttgacgtttt tgatttcctt gcctttgggt acaggaatcc caaattgcgc aaagagctgt
     1021 ttggcttgaa actcgtgaat attcataaat cctcttcgta ggttctacag ctcaaatcgg
     1081 aacattcggc gatgacaata tcccggttgg tgatcaatcg acttgataat tacttgttgg
     1141 tgtaggccgg caagaccatg ggtgagatga tgccagcgga cacatgatgc cgtttggctt
     1201 ccgctcgaaa ccgcttcaga tggtctaatg tcaatgaaca tttcggcaga gacttcgatc
     1261 gctcggctgc gagagtaccc gtccgcttcc caaacaccat taagtccaac aatgaattcc
     1321 ccatgagccg atttcgccca tgcaatcccc cggaggcctc tcctgccaca aataaattag
     1381 ccaccggcgt ttccccattc acatcaatct tgacgccccc gttttggtaa tgcagcgtgg
     1441 gataaatcaa cacggggtct ttccggatat caacctggta gcgctcatac tgccgaacca
     1501 tcgcgggaaa atgtttatcc aatgttccag ggccatgttc cacatccaac aatggtgtgt
     1561 cgagccagac tcccaccctt cctgatggtg ttcgaacgcc tcggccttct tcacattctc
     1621 gaatgatcga ggaggacacg acatctcggg tatccaattc attcacaaaa cgctcgccct
     1681 tcccattcac caaatggccg ccctcagatc gaatgccttc tgtgaccagc gcaccgacta
     1741 attgttccgg atacacgcca cctgaaggat gatattggaa ggtatcgatt tgaacgaggg
     1801 gcaccccgag gcgataggcc agaaccaggg catcccctgt cgccccgaaa tgattgctgg
     1861 tcggaaaacc ctgaatgtgc aaccggccta ttccgcctgt tgccaggatc accgttttgg
     1921 ccgcaacgac gatgtaccgg ttgttgtcta agtccttcag caccgccccg gtgcaggcgc
     1981 ccttggcatc gcttaacaat tccaccgccg cgacaaattc caacaacgga accttctggt
     2041 tcagcacttc gtctttcagc acacgcatga tttccaaacc ggtgtaatcg gagcaggtta
     2101 acagcctggc ctttgagctt cctccccctt ttttcacatg cagatttcca tcggcatccc
     2161 gatcgaataa gacacccaat tccagcagcc atttcgcaat agacggtccc tcttccacca
     2221 tgacctttaa caactggtgg tcatttttca tgtgcccgcc ctttaaggtg tccaagaaat
     2281 gctggacagg ggaatcgtcc gagccgacgg caatttgcat cccgccttgg gccatgaccg
     2341 agttggaatc cccgagccgt aacttcgtgg ccaacaacac attggcaccc tgggcatggg
     2401 catggagcgc cgatgcacac cccgcccctc ctcccccaac gaccaggaca tccacggaat
     2461 aatcaggagt aaggttaaga tcagccggaa ccgggctctc cccttccaac agagtcgcca
     2521 cttcggtcac ggttgattca ccggcattcg ggccaaaaat aattggccgg taggcatgag
     2581 cccgatgatc aggatggtat ttcttgataa ggccgtcccg ttcggctgga gaaagcttgg
     2641 gaaaactttt agaccggcgg acgtctcggg tttgatgaac taaatttctc tgatcggtaa
     2701 gttccatggg aaaagcgcag aaaactgttt aggttgtggc ggcggtggta ttttgtaatt
     2761 cctcatcgga caacttaagg atacgatccc attcctgctg aaaacgccca tcggtaatct
     2821 cttgaatacg tgtcgccaga ccttcaggtt tctcggtgaa atgtacgcct tgggcccgac
     2881 tggcatagag cgcgaccagg ttgggagcta tatcagcaat acacacgggc gcacacattc
     2941 cacacatcac acaatccatg aacatttccg agaccgcttt aaaatcccca aacacggctt
     3001 tccacacccc ttcccgcaca tcgatatgtt gcgggcaggc ttctgtgcag gcattacaat
     3061 ttcggcataa gggggcttca ggatagaggg taaacaaatc ctgtttggga tctttgagct
     3121 caagaagatt gtaggtcgct tttctcgcag gaaaagccgg catgaacgaa aaggacatgc
     3181 catcttcaac tgccatcgag cacgccaaac aggttttgac cttgggatcg tctttcgtac
     3241 ggtaataggt cgcacaggct ccacaaaaac cgcctaggca tccaacccca cggatcacat
     3301 cctgcccggc ataccacaag gccttgatca gggttatacc cgctggaacc tgaaacggct
     3361 tcccttcaat ctccaccgtg accatcttgg gttggagatt ctcttcttga tccagcgtat
     3421 tttttttatc caatgtgtca ggcatagctt agagatagta ccttaactct taacaaattt
     3481 ctcgtgatgc gttccaatca cctagcccca cttaattgag gggtagggtt aatttaacgc
     3541 attcaatgct gaaccggcct taaaccacgt aagctgttgt tccgtcatac tatgattggc
     3601 ttgaatgacc acttcctgcc catccttctt gtgaaggaca acctgaaccg gcttacctgg
     3661 ggcaagtcca cttaaccctt tcacactgat ccggtcttcc tgttcaattt tgtcataatc
     3721 cttcggatcc gcaaatgtca gtgcaaggat gccttgcttc ttgaga
//
LOCUS       DAORAQ010000243         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3401_length_3764_cov_58.767862, whole genome shotgun sequence.
ACCESSION   DAORAQ010000243 DAORAQ010000000
VERSION     DAORAQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_3764_cov_58.767862"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKM72_13515"
     CDS             complement(<1..193)
                     /locus_tag="PKM72_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61858.1"
                     /translation="MELKNLLTPWNWFKKEEEQSQSARSQNVSRSPDHPLARFHRDLD
                     QLFDNVFQGFPLSPGRRENG"
     gene            512..679
                     /locus_tag="PKM72_13520"
     CDS             512..679
                     /locus_tag="PKM72_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61859.1"
                     /translation="MGGIIEGVLALMGLTAGVIVALTALLVGDDPNSSEPKGSCRKSR
                     TILPGDERVAA"
     gene            782..1090
                     /locus_tag="PKM72_13525"
     CDS             782..1090
                     /locus_tag="PKM72_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61860.1"
                     /translation="MKKFIVVATVINLVLIGSLAFGNPGLLPKHPGYPMGNFKDPVLG
                     IPTANDPGETPPSPQEGLKQAAAFHDAQAINPMNEIRPNVVHEEQKTAPQGSATNSEK
                     "
     gene            complement(1251..1937)
                     /locus_tag="PKM72_13530"
     CDS             complement(1251..1937)
                     /locus_tag="PKM72_13530"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNP61861.1"
                     /translation="MIMQPQADDRTQTLLKVLVGSHAHGLASEESDRDYRRVYVIPTE
                     EMFQLGYKYPATKWTKGDGDETAWEVGNFLLLGTQGHPLILETLLAPIITADAWGEQL
                     RMLLPALWSPPKAFEAFTNYADNQRTKFLNKKDGRPAKYAAAYIRVLYNLCELLESGS
                     FHIRIADTPAGERLFRIKNGQYRLGEVMDWGEELTAQAGRLIAVCRDESDLPRINHFL
                     ATIRRAFLPP"
     gene            complement(1957..3252)
                     /locus_tag="PKM72_13535"
     CDS             complement(1957..3252)
                     /locus_tag="PKM72_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNP61862.1"
                     /translation="MMTIEESILGFLVALASGALIGLERQQNLRVERKTGIGGVRTFP
                     LIALAGAMSAFISQILGVWPIIATFLILGALLVVAYDKEWGRKDPPGIITPLAALITF
                     LLGVLALLPALPIDPPHRYLLIVGSAGVVMALLSLKEQLHHVVQQVSDGDIYATAKFV
                     ILALVVLPLLPNRTFGPLDVLNPFHTGVMVVLIAGVSFLGYLCTRIIGSHKGLATTGI
                     VGGLVSSTAVTISMATQAGENPRLVLPGATAILGASSTMFARMLVIVSLLQPDLFLML
                     LAPLGGMSLCGYVMSFILFNKAQRIPPDGPDISHRNPFELRPALGFGVLYAGVLFISK
                     AAQTYLGDQGLYASSLLAGTTDVDAIMLSIVRLQQDGLLAWTAATAITLAAMTNTIVK
                     LLLAGWFGGKPLIKYVAPGMIAILATGSVILIVFGFTHP"
BASE COUNT          959 a          889 c         1028 g          888 t
ORIGIN      
        1 ttccgttttc tcttcgtccc ggcgataggg ggaatccttg gaagacgttg tcaaacagtt
       61 ggtcgaggtc acgatggaac cgggccagag gatggtcggg cgacctggat acgttttgac
      121 ttcgtgccga ttgagattgt tcttcctctt ttttgaacca attccaggga gtaagaagat
      181 ttttgagttc catggtggat gtcctccttt catctaaaaa aactgttttg gaagaagtgg
      241 ttttcatgat tttcgcactg gttatgatat cccggtgaaa tgtggccggc catgcttaac
      301 tccttcagta gcaacaactt ctacaataga gctaatacat tttcgaaaaa agtcaagccg
      361 tcaacacggg agggaaatac catgtgcact cagccaataa agggaaagac atggtccagg
      421 gttttcccct cttgactttt tttggtcaga aattattaaa aatacagcaa cgaaagttga
      481 ttgaaattta cgatgaaagg aggtgaatgt tatgggtggt attattgaag gcgtgcttgc
      541 ccttatggga ctaacggcgg gtgtgatagt agccctcacg gctttgttag ttggagatga
      601 cccaaactca tcagagccaa aagggtcttg ccgaaaatcc cgtaccattc tccccggtga
      661 tgagcgggtt gctgcctaac gcagagattt tcaggcccgc ttatgagcgg gcctttattg
      721 attttctttt ttcctcgctc ctgttcctgt ttcggccaag agagaaagga ggatgtcaat
      781 tatgaaaaaa tttatcgtcg ttgcaacagt gataaatctg gtgttgattg ggtcactggc
      841 attcggaaat cccggtctac tcccgaagca tccgggctat cccatgggta atttcaaaga
      901 ccccgtccta ggcatcccca cggcgaatga cccaggagaa accccaccat caccgcagga
      961 gggattgaaa caggctgcag cgtttcatga tgcccaggca atcaacccca tgaatgagat
     1021 tcgcccgaat gtggttcatg aggagcaaaa gactgcgcct caaggatctg ctacaaatag
     1081 cgaaaagtaa tttgacgcta atgttcaacc acgaaaccat aaactcggag tcaggaccag
     1141 gggtatggaa agaggggatc gaatgatccc ctctttttgt gatgtatcat cgaaaggccc
     1201 gagaagatga aacgttatca tggaaaaggg caggcatttt taacgtcttg ttaaggtggg
     1261 agaaatgccc ggcgaatggt tgccagaaag tgattgatgc gtggcagatc gctttcatcg
     1321 cggcatacag caattaatcg acctgcctgc gctgtaagtt cctcgcccca atccattact
     1381 tcccccaacc ggtattgtcc atttttaatg cgaaacagtc gttcccctgc cggcgtgtct
     1441 gcaatgcgga tgtgaaaact tcctgattca agcaattcac agaggttgta gagaacccgg
     1501 atataggcgg ccgcatactt tgccggacgt ccatctttct tattcagaaa tttggtgcgt
     1561 tgattgtcgg catagttggt gaaggcttca aaggctttgg gaggcgacca tagggccggc
     1621 aacagcatgc gcaattgctc tccccatgca tcggctgtga tgatgggagc cagcaacgtc
     1681 tcaagaataa gcggatggcc ctgtgttccc agcagaagaa aatttccgac ctcccaggcc
     1741 gtttcatcgc catccccttt ggtccacttc gtggcagggt atttatatcc taactgaaac
     1801 atctcctccg tcgggatgac atagacccgg cgataatccc ggtcgctctc ctcggacgct
     1861 aagccatggg catgggaacc cacgagaact ttgagaagag tctgtgtacg atcgtctgcc
     1921 tgaggctgca tgatcatttg tcgtaaattt ccattgttaa gggtgtgtga agccaaagac
     1981 gatcagaatg acactcccgg ttgccaaaat ggcgatcatt cccggcgcca catattttat
     2041 gagcggtttt cctccaaacc atcctgctag cagaagcttt acaatcgtat tggtcatggc
     2101 tgcgagggtg atggccgtgg cggcagtcca ggccaaaaga ccgtcctgct gtaatcggac
     2161 aattgacaac atgatggcat ccacgtcggt agtgccggcc agaagactgg acgcatacaa
     2221 tccttgatcg cccaggtagg tttgggcggc tttcgaaatg aacaggactc cggcatacaa
     2281 tacaccaaat cccagggccg gacgtaattc gaacgggttg cgatgggaga tgtccggacc
     2341 atccggcggt atgcgttgcg ctttgttaaa taagatgaaa ctcatgacat acccgcagag
     2401 ggacatgccc cccaaaggcg cgagaagcat gagaaacaga tccggttgca ggaggctcac
     2461 gatgaccagc attctggcaa acatggtact ggacgcgcct aggattgccg tcgctccagg
     2521 gaggaccaga cgtgggtttt ccccggcttg ggtagccatg ctgatggtga cggcggtgga
     2581 agagaccagg cctcccacaa tgcctgttgt ggcaaggcct ttatgggaac cgatgatgcg
     2641 agtgcagaga tatcctaaaa aactcacccc cgcaatgagg accaccatga cgccggtatg
     2701 gaagggattg aggacatcca gaggaccgaa agtgcggttg gggagaagag gcaaaacgac
     2761 gagggctaaa atgacaaatt ttgccgttgc atagatatca ccatcggata cctgttgcac
     2821 cacatgatgg agttgttctt ttaacgacag cagggccatc acgacccctg cgctgccgac
     2881 aatgaggaga taccgatgtg gtggatcaat cggaagagct gggagtaggg cgagcactcc
     2941 cagaagaaac gtgatcaggg cggcgagtgg ggtgatgatc ccaggtggat cctttcgacc
     3001 ccattcctta tcgtacgcga caaccaggag cgcgccaaga atcaggaatg tggcaataat
     3061 gggccacacg ccaaggatct gggagataaa cgccgacatg gctccggcaa gggcgatcag
     3121 gggaaacgtc cgcaccccgc cgataccggt tttgcgctcg accctcagat tctgctgacg
     3181 ttccaagcca attaaagccc ctgaggcgag ggctaccaga aagccaagaa tggattcttc
     3241 aatagtcatc atgggcaggt cttattgaag caggaaatcc agaggaaaag attgagaact
     3301 atcatctatc tgatggtcac caatactcac tcaatatgaa atgagaagca agaagcgggc
     3361 tcaatcgggt agagaacaga tttaagcgtt cttgaaaagc gcccttgtga aattgtccga
     3421 tatggaccgg ctcccttttt tagtggacca caacatgtcg agtttttttc aggcggtggg
     3481 aggacgttcc ggtccgggga tcatttgatc cggtatgatc tggcgtggtt gcttcaataa
     3541 atttcgagta aggggcggag gggttctgtg gggcgtccga acccggcaaa cccgagtctc
     3601 tgtccgccgg gtattattca gcagataggg tcaaggggat agggggcggg tagtatcggc
     3661 gatagcaatc ccggcctgtt tttcaagcga gagttgaggt ctttatctga catgtaagac
     3721 tcccgcgccc tggatggttc cggcctttaa ttgttggagt gcca
//
LOCUS       DAORAQ010000244         3758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3416_length_3758_cov_64.384013, whole genome shotgun sequence.
ACCESSION   DAORAQ010000244 DAORAQ010000000
VERSION     DAORAQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3416_length_3758_cov_64.384013"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1174
                     /locus_tag="PKM72_13540"
     CDS             380..1174
                     /locus_tag="PKM72_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNP61863.1"
                     /translation="MILFGPSWCYGQESNREFSSPRPWIMGQAGAFTEPIETDRPTFS
                     LSPATITKGRIQIETGYTFSFEHASPGVKTHNFPETLVRIGLTERTEFRLEWPNLTFI
                     DNGRDVHGFNDLGLGFKVQVWQQQGLRPRLSFVGRLSIPTGDKNFSSDKLDPILRTIL
                     TYALNEKVGLFGTVNIGSPTSQGTRFVQVSSSLGLSAALRDRLTGFVEYFGLYPRDVA
                     SGSANFLQTGLIYRLTYNLQIDARIGGGLTHGSDDLLTGAGISWRF"
     gene            complement(1247..2008)
                     /locus_tag="PKM72_13545"
     CDS             complement(1247..2008)
                     /locus_tag="PKM72_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP61864.1"
                     /translation="MHTCSASRASICCQSILLLSLTVWLIGPSPSIRPETLQGVPASP
                     PSSEICTAHAPVTNECVEAGLRARREVIRAEPAFQVKTPATHLQLAERLNQQGDPNGA
                     IEEYLAAIQLDPDLAAAFQGMGAVYLDQHEWKKAEEVLERAAQLDSGNNQTEYWLGRS
                     LLAQHKFPEAQEVLRKTTRLKPDDAEAFSDLGLTYMAQGQSQNAMKALRQAIRLRPDF
                     SEAHSRLELAETFKLNKDQLVLETDKILRILFRRE"
     gene            2369..>3758
                     /gene="kaiC"
                     /locus_tag="PKM72_13550"
     CDS             2369..>3758
                     /gene="kaiC"
                     /locus_tag="PKM72_13550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNP61865.1"
                     /translation="MPRKKSSSNTVHSEFPKALTGIQGLDEITGGGLPRGRTTLVCGS
                     PGCGKTLLGMEFLVRGIVESGEPGIMLAFEETAEELTQNVRSLGFDLDRLIKDNKLIL
                     DTIPIEATEFEENGEYDLEGLFVRLGDSIDTIGAKRIVLDTIEVLFGGLSNQGIIRSE
                     LRRLFQWLKDRNMTSIVTGERGEGLLTRQGLEEYVSDCVILLDHRVNDQMSTRRLRVV
                     KYRGSMHGTNEFPFLIEQHGLSVLPITSLKLEHKISKEKISTGVPQLDEMFGGEGVYR
                     GSTVLVTGGVGTGKTSLVSHFADAACRQGEQALYFAFEESSPQIMRNMKSIGLSLDKW
                     VNKGLLHILASRPTLFGLESHLVNFYNAVKTHHPKLVIMDPINDFLSIGSTREVKALF
                     LRMVDFLKSQQITLVCTGLSAPSEPAQAAEIGVSSLADTWILLSNREFDGEHQRQLYI
                     LKSRGMAHSHQVR"
BASE COUNT          900 a          856 c         1016 g          986 t
ORIGIN      
        1 gggcggccat gtatgtgacc aaaagcagtg tcgccaacac actggtcgat gccatccgtg
       61 atgtcgtaaa acaaacttgc taaacctttt gtgtaatgac ttatgagaat cgttttagac
      121 gggcttcatt gagacaacac ctcacagtca aagccggata ttacgccagg gtgtgatctc
      181 ataggcgcgt ttgggattgc tacccggcgt aaaaggtggc cccatcttat ctctccctga
      241 acgacagcca atcaaggtca ttaagggtag gctttagagt ttttttaaca gaactccagt
      301 ttctatgcgt atctcctatg ttgtaaccat ttcaagaatt ggacggtcgc tctctggtgt
      361 tctagggtgg tcgttgtgtg tgattctgtt cggaccatcc tggtgttatg gtcaagaaag
      421 taatcgtgag ttttcttcgc ctcgcccgtg gatcatgggc caggccggcg cttttaccga
      481 gccgattgag acggatcggc cgacatttag cctgagtcct gccacgatca ccaagggacg
      541 cattcaaatc gaaacgggat ataccttttc ttttgaacat gcatcacccg gtgtcaaaac
      601 gcataacttc cctgagaccc ttgtccgtat tggattgacg gagaggactg aatttcgcct
      661 ggaatggcca aatctgacct tcattgacaa tggcagggat gtgcatggtt tcaatgatct
      721 tggcctggga tttaaagtcc aggtctggca gcaacaaggg ctcagacccc ggttgagttt
      781 tgtcggccga ctgtcgattc ccacgggaga taagaatttc tcttcggata agctcgaccc
      841 gatcttgcgg acgattttga cgtatgcgtt gaatgaaaag gtagggttgt tcgggacggt
      901 gaatattggc agtcccactt ctcagggaac gcggtttgtg caggtttctt cgtcactggg
      961 gctcagcgca gcccttcgag atcggttaac cggattcgtg gaatatttcg gattgtatcc
     1021 cagagatgtg gcgagtggga gcgccaattt tcttcaaacg ggattgatct atcgtttgac
     1081 gtacaatttg caaattgatg cacgcatagg aggcgggctg acccatggtt ctgatgatct
     1141 gttgacgggt gccgggattt cgtggagatt ttaacccgat cgaaaacgaa gacaggtgtc
     1201 gttcgttcac ctgacacaga gttgcgctca ggaacgaatg atacctttat tcccttcgga
     1261 aaagaatccg taaaatttta tcggtctcaa ggacgagttg atctttgttg agtttgaagg
     1321 tctcagccag ttccaggcgg gaatgggcct ctgaaaaatc cggtcgtaag cgaatggctt
     1381 gccggagtgc cttcatggca ttctgggatt gtccttgggc catgtacgtc aggcctaaat
     1441 cggaaaaagc ttccgcatca tcgggcttca gcctcgtcgt cttcctcagc acttcctgag
     1501 cctcaggaaa tttgtgttga gctaaaagtg atcgacccag ccagtattcg gtctggttat
     1561 ttccgctatc taactgcgcg gctctttcaa gcacttcttc tgccttcttc cattcatgtt
     1621 gatcaaggta gacggctccc ataccctgaa acgcagcggc cagatcggga tcaagttgaa
     1681 tcgcggctag atattcctcg atggcgccgt tcggatcgcc ctgctgattg agtcgttccg
     1741 ccaattggag gtgagtcgcg ggggtcttaa cctggaatgc gggttccgct ctgatcactt
     1801 cacgtctggc ccgtagacca gcttccacgc attcgttggt gaccggagcg tgcgcagtgc
     1861 agatctctga agatggtgga gaagcaggga ccccttgaag ggtttccggg cgaatggaag
     1921 gtgaaggacc tatcaaccat accgttagag aaagcaggag aatggattgg caacagatgg
     1981 aggcccgaga agctgaacaa gtatgcatga gccaaaccac taggggatga agggtaaagg
     2041 ttttttaatg agacggatga atgtacggaa tgaagaaaaa attgccgaaa tattcgacaa
     2101 aatactccga attcggtcta ttctgtccat tcttctgtgt ccacatgaac tgatacggtg
     2161 tctcctaacc tgctaaagtg acacataaaa gtgactgccc gacgctgaaa aggatttgag
     2221 atagggtaac caattgatga gttgtagggg aatcaatcat ttatcgttgc cgttggaatc
     2281 tcgagttttt cgccaatttg tattattttt taaagatatt atgttagatt ttgacgtcaa
     2341 ttttttccat ctcaggaaac tataaatcat gccccgaaag aaatcatcaa gtaacacggt
     2401 ccattcagaa tttcccaaag cgctcaccgg tattcaaggc ctggatgaaa taaccggcgg
     2461 cgggcttccc cgaggacgaa cgacgttggt ctgcggaagt cctggctgtg ggaaaacact
     2521 tctgggcatg gaatttcttg tccgcgggat tgtggaaagc ggagagcccg gtatcatgtt
     2581 ggcgttcgag gaaacggccg aggaattgac ccaaaacgtc cggtccctgg gatttgatct
     2641 agatagatta atcaaggata acaaattgat tttggatacg attcccattg aagccaccga
     2701 atttgaagag aatggagaat acgatctcga gggcctattt gtgcggctgg gtgattccat
     2761 cgatacgatc ggcgccaaac ggattgtgtt ggacaccatc gaggtcctgt tcggcgggtt
     2821 atccaatcag gggattatcc gttccgagtt gcggcggctg tttcaatggc tgaaagaccg
     2881 gaacatgacc tccattgtca ccggcgagcg gggagagggg ttgttgaccc gtcaggggct
     2941 tgaggaatat gtgtccgact gcgtcattct gctggatcat cgagtgaacg atcagatgtc
     3001 cacccgtcga ttgcgtgtgg ttaaataccg agggagcatg catggcacca atgaatttcc
     3061 ctttctcatt gaacaacatg ggctgtctgt cctgcccatc acgtccttga aacttgaaca
     3121 caaaatttca aaagaaaaaa tctcgaccgg cgttccacag ttggatgaga tgttcggggg
     3181 agaaggtgtg tatagggggt cgacggttct ggtcacaggc ggagtcggga cggggaaaac
     3241 gagcctggtt tcgcattttg cggatgctgc ctgtcgccag ggagaacagg ccttgtattt
     3301 tgcctttgaa gagtccagcc ctcagattat gcggaacatg aaatcgatcg ggctctcgct
     3361 ggacaaatgg gtaaacaagg gcttattgca cattctcgcc tcacgaccca cgctattcgg
     3421 cttggaatcc catctcgtga atttttataa tgccgtgaaa acccatcatc cgaagctggt
     3481 gattatggat ccgatcaacg attttctatc cattggttca actcgcgagg tcaaggcgtt
     3541 atttttacgg atggtggatt ttctgaaatc ccaacaaatt accttggttt gcacgggcct
     3601 cagcgcgcct tcggaacctg ctcaggcggc ggagatcggg gtttcctcac tggccgacac
     3661 ctggattctc ctctcaaacc gggaatttga tggtgagcac cagcggcaac tgtatatttt
     3721 gaaatcccgg ggtatggcgc attcgcatca ggttcggg
//
LOCUS       DAORAQ010000245         3751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3426_length_3751_cov_64.037067, whole genome shotgun sequence.
ACCESSION   DAORAQ010000245 DAORAQ010000000
VERSION     DAORAQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_3751_cov_64.037067"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1186
                     /locus_tag="PKM72_13555"
     CDS             131..1186
                     /locus_tag="PKM72_13555"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNP61866.1"
                     /translation="MGSTKGYAALQAGATLEPFSFDRRPVGSNDVLIKIDYCGVCHSD
                     IHQARDEWGGSIFPMVPGHEIVGTVTIVGKDVRKVRQGERVGVGCFVDSCRTCPACTQ
                     GVEQYCEPGMIPTYNGLDKNGHPTYGGYSHHIVVNEQYVLKIPETLSMEGAAPLLCAG
                     ITTYSPLRQWGVGKGHKLGVVGLGGLGHMAVKFGKALGAEVTVFSRTDQKRVEAERLG
                     ATAFAATSQEGTFRRLAGQFDFVIDTVSGQHDYNAHLELLKTDGTYILVGAPSEPIAL
                     GAFQLIMRRRRLVGSLVGGIRETQDMLNFCGEHGITSDVEVIPIQQINEAYERVLRGD
                     VRFRFVIDMASLPDTPE"
     gene            1492..2583
                     /locus_tag="PKM72_13560"
     CDS             1492..2583
                     /locus_tag="PKM72_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP61867.1"
                     /translation="MMGMVVHLVILLMLVWPTDSLARRASFTSEQKTQLSSIQIIRVS
                     TLALSENGFVSSEIILNVVRRRFEELGFQVVTDAAQPHDVEFHVICEERKHEQSVARY
                     GGDAELTHTPDRLWYGPACQLSYRLERKDLGWHKEVRASIQEDSVLPREAQAVDIGAS
                     VFEQLTRELETLDFPVMLLSEWGQTHRLVALLTDPETPQTRQMVILDLLRQFPDREAL
                     PYLLKRIEQGHSPHESIAALSGLGHEVVPHLQHLFECQDQPVPIRAAAAKGLGRILRA
                     DGDAEVMTMLLKYLSHAVLEIRSSSDIEFPVLTEVVWAVGSIHHQPTVKLIGTLQDRI
                     WLIYDNSPEMKKLRDVVSVVHKYLDPSQI"
     gene            complement(2870..3184)
                     /locus_tag="PKM72_13565"
     CDS             complement(2870..3184)
                     /locus_tag="PKM72_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61868.1"
                     /translation="MTLHAHAEALYAGIRSLIGQHDANQVQATLERDLRHMIPTWNHT
                     TPDCPGWYWFKAPGVSEPRLLYVGHGRIDTKLMADLGPPNNLVDVQRIKGQWAGPLLP
                     PT"
     gene            3372..3605
                     /locus_tag="PKM72_13570"
     CDS             3372..3605
                     /locus_tag="PKM72_13570"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HNP61869.1"
                     /translation="MSRNLSKVIHGNNRIGLLGNHTGGTMDWVPVMGYVAEACTTLLF
                     LPQAIQIVKTKQTKDFSLSMYSMLTSGLVLWSV"
BASE COUNT          856 a          901 c         1032 g          962 t
ORIGIN      
        1 tgggaatttt gtccgtgagg gatctgatgc gaatgatttc cgtcagggat cgcccaagat
       61 ttctcaatca gccatcataa tgctccttcc ctctggcagg attgctttga ccaaagaaag
      121 gagcgcaacc atgggaagca ccaaagggta tgcggcgttg caagccggcg ccacactcga
      181 accgttcagc tttgaccggc gaccggtcgg atcgaatgac gttctgatca agatcgacta
      241 ttgcggagtc tgccattccg acattcatca ggcgcgtgac gaatggggcg gatcaatttt
      301 tcccatggtc ccgggccatg agattgtcgg gaccgtgacg attgtgggga aagacgtcag
      361 gaaggttcgg caaggtgaga gggtcggagt aggatgtttt gtcgactcat gtcgcacctg
      421 tccggcatgc acccagggcg tagaacaata ttgtgaaccg ggaatgattc cgacctataa
      481 cgggctggat aaaaacggtc accccaccta tggcgggtat tcccaccaca tcgttgtcaa
      541 tgaacagtac gtgctgaaaa tcccggaaac tctctcgatg gaaggtgcag ccccactctt
      601 gtgtgccgga attacgacgt actctccgct gcgacaatgg ggtgtgggaa aaggacataa
      661 gctcggggtc gtggggttgg gggggcttgg ccatatggct gtgaaatttg gaaaggccct
      721 tggagcggag gtcactgttt ttagccgcac cgaccaaaag cgagtggagg ctgaacgtct
      781 tggggccaca gcatttgctg cgacttcgca ggaggggacg tttaggcgtt tggcgggtca
      841 gtttgatttt gtcattgata cggtttcggg ccagcatgat tacaatgctc atctcgaatt
      901 actgaaaacc gatggcacat acattcttgt cggtgcgccg agtgagccga ttgcccttgg
      961 ggcgtttcag cttatcatgc ggcggcgacg tctggtgggc tccctggttg gcgggattcg
     1021 cgagacgcag gacatgctga atttttgcgg ggagcatggc atcacatctg atgtggaagt
     1081 cattcccatc caacaaatta atgaagccta tgaacgggtc ctgcggggcg acgtccgttt
     1141 ccgtttcgtc atcgatatgg catcgttacc tgatacgcca gaatgaattc gttttttttc
     1201 gtgttcgttc ggtctcatcg ttttccactt ctcttaggaa atcttcggga agtgagccct
     1261 ggtgtataat gaggccgtag tgtccggctg tatgagtgat gatgcggtcc cattggaaat
     1321 tgtctgcaat tgtccactat tccgcctgaa atacttgttt ccctgctgct tgtgaagcaa
     1381 taggcgttgg gagagggaat ctagccttta tccgcaaact ttttccttcc accaaaaaaa
     1441 tgtttgcgat taaacgaatc tctggtagtc ttgaccataa gggagattgc catgatggga
     1501 atggtggtgc atctcgttat tcttctgatg ttggtctggc ccacggacag tttggcccgc
     1561 cgcgcctctt tcacttctga acaaaaaaca cagctctcca gcattcaaat aattcgggtt
     1621 tccaccctgg cgctgagcga aaatggcttt gtgtcctcgg agattatcct caacgtggtc
     1681 cgacggcgat tcgaagagct cggatttcag gtggtcacgg atgccgccca accgcatgat
     1741 gtggaattcc acgtaatctg tgaagaacgg aagcacgaac aaagcgttgc acgctatggc
     1801 ggagatgctg agctgaccca tacgccggac cggctctggt atggcccggc ctgccagtta
     1861 tcttatcgcc tggagaggaa ggaccttggg tggcacaaag aagtgcgtgc ctccattcag
     1921 gaggattccg tccttccgcg agaggctcag gccgtagaca tcggagcttc tgtctttgaa
     1981 caactcacac gggaacttga aacacttgat tttccggtta tgctactgag tgaatgggga
     2041 cagacgcatc gactggtggc cttgctcacc gatcctgaga caccccaaac ccgtcaaatg
     2101 gttattcttg atttgctccg tcagttccct gaccgggaag cccttcctta tcttctgaaa
     2161 cgaattgaac aggggcattc ccctcatgaa tccattgccg ctctgtccgg gttgggccat
     2221 gaggtggttc cgcatctgca acacctgttt gaatgtcagg accaaccggt cccgattcga
     2281 gctgccgctg ccaagggttt aggaagaatt ttgcgagcag acggggatgc tgaggtgatg
     2341 acgatgttgc tgaagtatct gtcccatgct gtgttggaga ttaggtcttc ctcggatatc
     2401 gaatttcctg tgctcacgga agtcgtgtgg gctgtcgggt ccattcatca tcaaccaacg
     2461 gtgaaactga ttggcactct ccaagaccgg atctggctca tttacgataa ctcaccggag
     2521 atgaagaagc tgcgggatgt ggtgagcgtt gtccacaaat atttggaccc cagtcagatt
     2581 tgaaatgggg aacgggagtt ggaccatatg accactagga gtggctgaag aaacaaggta
     2641 gattttaaga ctgttgaatc attttaaatt ccccctagtt ccagccctaa ctatttcatt
     2701 gctgagcaat cgagagcaat gcggagagtg ttcaaaaagc ccgtccagta aggcatcagc
     2761 aagttagcgc gcggagcgta cacgcaagta cgtgagcatg acaacgaggc gagaacgccg
     2821 ccggcgggcc cttcggcccg actcagggca tgttttttaa cactctcact taggttggtg
     2881 gaagaagggg ccccgcccat tgacctttaa tgcgctgcac atccacaaga ttattcggcg
     2941 gacccaaatc tgccatgagc ttggtgtcaa tcctgccatg acccacatac aatagtcggg
     3001 gttcactcac gcccggcgct ttaaaccaat accatccggg acagtcaggt gttgtgtgat
     3061 tccaggttgg aatcatgtgg cgcaggtcac gttccagtgt agcttgcacc tggttcgcgt
     3121 catgctgacc gattaatgat cggatgcctg catatagtgc ttccgcatga gcgtgcaatg
     3181 tcattgtcct gagaatcctt tcttttaaac ctttgtgaaa gatcgccatc caacatacgt
     3241 cattttcttt ccgggactca actggttgca tgatccggaa aacatgtgac gcctctttcg
     3301 gctcaatggt tttgccaatc aaaaatctgt catctcgttc gtcctatctg acaatgggta
     3361 gagtgcacgg catgagcaga aatttgagca aagtgattca cgggaacaac cgcattggct
     3421 tattgggaaa tcacacagga ggcaccatgg attgggtacc cgtgatgggg tatgtcgcgg
     3481 aggcatgcac gaccttattg tttcttcctc aggcgattca aatcgtaaaa acgaaacaaa
     3541 ccaaagattt ttccctgagt atgtattcaa tgttgacttc gggcttggtt ctctggagcg
     3601 tgtagggatt gatcaatcca gattggccat tcgtcatcgc cattatggtg gcgctgtttc
     3661 tagctggttg gattttcatc ctcaagctcc ggtacgggta gagtgcaaag atgcggaaat
     3721 ttcgccttgt ttcagaatgg tgatggtcgt g
//
LOCUS       DAORAQ010000246         3747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3432_length_3747_cov_67.213164, whole genome shotgun sequence.
ACCESSION   DAORAQ010000246 DAORAQ010000000
VERSION     DAORAQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_3747_cov_67.213164"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1851..2120
                     /locus_tag="PKM72_13575"
     CDS             1851..2120
                     /locus_tag="PKM72_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61870.1"
                     /translation="MVKFGAAPAIVEEEIIDSIRKRVHNGAVFLPPLASFESGQIIRI
                     NHGPFHGLEAIFEQNRTEWDPTSFTSYETMALQGRMVIGCKCVVL"
BASE COUNT         1058 a          693 c          886 g         1110 t
ORIGIN      
        1 ggatcaagcc cggaatgggc agtggccagc tggacgaggc caaagcatcc gagtgccagt
       61 gccaaagagg ctattgccac gacggaagat ctgtgtcgag atctgacttg ctggtcaatt
      121 ttaatggaaa cagacatgag tctttccttt tcattattgg gaaatagggg catcttgctc
      181 atccgtgcgc ctctctataa gggggtactg ctttagattg atgcatcttc gatttgaaaa
      241 tagttgtcat gttggtctgg tcgacgataa atttttcctg gttttcgcga gccttttgta
      301 caggcggtaa cttctttgat taagagaaac ataattgtag acagtttatt atgaaattgt
      361 aaaagaagcc aatttccgaa gctaaatttt cagggatata taaacccctg ggcaatgcct
      421 cttttaacct atcggttttt ttatctccga aagtacggta tgttcagcga ggcactcgac
      481 ttggtgtttg agtcctcggc cgaccccctt actcccacct ggtaagaggg tctggcctat
      541 ttgctgcctt acactttttt agcgcaaatg ggaggacata aggatctgtg ttacgtcatg
      601 accgtccagt ccaggaagtt acataaggtc aggccatctt cagtgtcgac cgaattggtg
      661 aacaatttgt ggttcagact tgggaaacta tgctgaagag gtttttttgg cgatcgtagg
      721 tgtctcctca aatcgccgtc tggatatatc cagaaattgg ttgtggaact cccgaaaatc
      781 taggcattgt gattacctaa tccagggccc gactagtcta tgccaacgat ttttgcctaa
      841 agcaagaaca cttatctggt gcaaaacatc atggtttcct ttggaacctg cagtcgtacg
      901 tcctaacggt caataacggt aatgaggatt ctctggttct ctccctcaat aataccctcc
      961 atcagccttg ttctttccct gacctgattg ttaacagcaa gggtagaggt gaaaacacta
     1021 gcttttctac gtttactccc tagcagtgat aggcacctat ggctcaaacg ccacctagat
     1081 cagtcagttg acggcatccc tatcgcgctc tgttgtgtgg tgtggtcgta gaagctgcat
     1141 tgcagcagtc ctgaatggga gcgcatagca gtttgtttag cctggtctga ggcagggatc
     1201 gtcaaaaact cccaatagac aattggggac caaaaaacct ggaagaaaat taacgtggag
     1261 ggagtctttt tctatttcat cgttcctcct gaatgagtac tgccgagtat tctgccacag
     1321 atctaaaaca atattacaga tttcgttgcc tggaaccaaa tacaggatgg cttaagaaat
     1381 tagagcctca agctccgttg atttatccac ttcaatatgg ttggaggttg aaccaactaa
     1441 aaggtcgggg caattgttat tggagcagaa aaatgcctat gaggttaaag gagaagtgtt
     1501 gaatgagaaa tacattcctg tttatttaag aaatgctaac gaaaaaattg gagtaaagcg
     1561 ttgagttaat tgaattttct gatatgctct aatgaagatg gagacaatat aatgtcggac
     1621 tctcggtggg atctggttca tagtaaagct cgtcaggaag acgtggcgga ggccaactta
     1681 cagttattgg gtgtttatat cttctgtcca agttaagagg aaagagatta aaatcgactc
     1741 gaaataaggc gaaaagtgat ggagaggtat tattcccagg gtatatcttt atatgagttg
     1801 acatgaagac cgatatcgga aagtgcctta tgctcatggg gttctgaggg atggtcaagt
     1861 ttggtgccgc accagcaata gtggaagaag aaatcattga ctcaattagg aagagagttc
     1921 acaatggagc tgttttcctt ccccctttag cttcctttga atctggacaa attattcgta
     1981 ttaatcacgg gccttttcat ggccttgaag caattttcga acagaacaga acggaatggg
     2041 acccaacgag tttcacttct tatgaaacca tggcgttaca aggccgtatg gttattggtt
     2101 gcaagtgcgt ggtgttgtaa tgagaattga tacggcccat ttggccgtta cggggaaaat
     2161 tgggtcgaag gcaaatttag tcaatagggt ttaataactc attaattata ttgagcgact
     2221 atacctactt ttttcttcat acatgaagaa gcattcaagt ataatagcct aagttacgac
     2281 aaggacaatt caaaaatggt aagaaacaat ttgccattac atgccatagt ttcttgatca
     2341 ttttgaataa tggaatcaga agggtaggtg ccgtgagttc taaagtgctc ccattagtct
     2401 tcttcttgtc gggctgagta attttggtat ttgtttgagc gggaaaattt gaccacaagg
     2461 ttgttaacgc agttagctag gtatggcgtc agcttggaaa tttttgtcac agggaggaat
     2521 tgccatcacc tcaaggctaa acgtgaagtc atgatttttt tcgggtaaat ttggtggaaa
     2581 tggcaattat tgttgcacaa ttgagttcgg atgttttttc ttcagggaaa actaggccaa
     2641 gccaagtagg aatttagaag gacttaaatc ggcggtcaga tgaaatgccg actgtatttt
     2701 tcttgatttc ccacatgtag gaatgaatcg tctatgtagt ggcctgtttc aatgtgtacg
     2761 aatttttctc cttgtttaat agatggataa tcttagtaaa ttttgtgagg atgtcattga
     2821 caaagttctc aaggttgatt ctgtcagggg tttatggctt aaaatttctt ctcgagtctg
     2881 ttctaggcaa ataaatgata acctaagatt ttctggttat tggtgctgga atcatcggta
     2941 ttaatgcccc cccgacaact gaaacggcag ttttacgatg cgacggggac tgttattgaa
     3001 aacggaaatc actgtgacac tctcgccagt tgtcggaata gcggggtgtt gcacgttggt
     3061 ttttattaat tcaccggaaa gtcaaattta cacgcatagg aagtaaacgg ctaatgggat
     3121 ggtgtgaatc caagcagatt tcgatgaata ggcttggaaa gttcattgtt accaaagatg
     3181 gtggcgaaca tttggctcaa gaagaacttc tcaggtgagg gaaaaaacga cattgatata
     3241 caggctatta ataccgagga agaagcaaac ccattgagcc tagtgtgaaa cttaccaaca
     3301 agctctgttt tattaacaat atcaataatc gatccaattg aagatatgca atcaatgcct
     3361 agggatgcca ttgatggatg ggtacaggtt cgttgcgcaa attagtatct caggataagg
     3421 aaggaatgtc tttacctcag gcggtgcctt tatatgtagg gtatttcatt tttgagccta
     3481 gactttatgc agacaaaatt gaaaaggatt tcggtttttt cgagaattat aaaaatttgc
     3541 cattttttaa aggactctcc tactcggatg agccaagagg agccatccga acaaattttc
     3601 atccatctcc aaatttgcga aatccttttc tcagagctcg tgtcacagtc aactcggagg
     3661 gaaagcgaag attagaccaa ccgcgattcc gaccttttgg cgggaacaat ttttctgaat
     3721 cgagaaattt agccttcaag agttgag
//
LOCUS       DAORAQ010000247         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3452_length_3731_cov_71.895267, whole genome shotgun sequence.
ACCESSION   DAORAQ010000247 DAORAQ010000000
VERSION     DAORAQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3452_length_3731_cov_71.895267"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1971
                     /locus_tag="PKM72_13580"
     CDS             418..1971
                     /locus_tag="PKM72_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNP61871.1"
                     /translation="MGVEIAISVTREETRVAVLDNRVVTELYVDRPRKKDFVGDIYKG
                     KVIKVLPGMQAAFVDIGLDRAAFIHVSDLSVGTEPGDTLVDSEEDERSQDLPRPRRQT
                     ARPIENLLTEGQELLVQISKGPIGTKGPRITTYVSLPGRFLVLMPNVDHIGVSRRITK
                     EEERHRLKDLMRRIRKPEYGYIVRTVCEDVPEDDLQTDVRFLTALWEDTLKAAGKQPA
                     PSLLHTDLSLPLRVVRDLFTKKVDRLLVDAKPDFEEVKDFVRRYLPEQASRVHFYDKK
                     QEGLFDHLGIELEITRALSRKVWLKSGGHIVIDHTEAMTVVDVNTGRFVGKRDQEETI
                     LKNNLEAAKEIGYQIKLRGIGGIIIIDFIDMERERNREKVYQATLDALAGDKAQTRIS
                     RISDLGLIEISRERVREDLLRTLSEICGDCEGRGYTKSTMTVAYEIFRDIRRIGITRG
                     QPQQIVVGANPKVIELIFETEHSSIEQLEQEFQQQILFEADPLLHLEQYDIVVVGKLA
                     SRAESRTAS"
     gene            complement(1984..2148)
                     /locus_tag="PKM72_13585"
     CDS             complement(1984..2148)
                     /locus_tag="PKM72_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61872.1"
                     /translation="MRLETTVRWFVRMALYQEIIPPSTPDDGVFRIFQELKEGFGSPL
                     LSVHGGKWYS"
     gene            2175..3311
                     /gene="dprA"
                     /locus_tag="PKM72_13590"
     CDS             2175..3311
                     /gene="dprA"
                     /locus_tag="PKM72_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNP61873.1"
                     /translation="MTSPLQGWLELLAVKGLGPVTYTRLINRFGSPDAIRSSTAHTLE
                     SIGEISPSLARALHQPISTDTQNQIAKELQAVQDGRFSILTLVDALYPPRLKTITDPP
                     PLLWYTGQLQDRDQQALAIVGSRKGSHIGRAFTRQLSGDLAALGFTIVSGMARGIDAA
                     AHEGALATSGRTLAVLGCGIDQTYPPEHDPLRQRIEQHGAVLSEFPMGTPPHSYHFPQ
                     RNRIISGLSLGVIVTEATSRSGSLITARMALDQNREVFAVPGNVTNSLTRGPHRLIKE
                     GAKLVENSLDVVEEILPMLEPSFRDQLEKQQGGFQPQPSTPALGAEEQHLFDCISLEP
                     VSLDDLINQCSYAPPEVMSILLSLEIKGLIKQIPGLQYLRMSIG"
BASE COUNT         1031 a          940 c          882 g          878 t
ORIGIN      
        1 atgagctatg gaggcagcgc catggtggtg aatctcacag ctttggggct cttgctcaac
       61 gtaaaacgga gacgcctgtc actgctttag gtatcatgta ccctgcggtt tggttttgat
      121 agtgttggac tggttatcgc ataaagaggg aaacagggaa aaattcaatt cagaaaattc
      181 caaaattccg tggattttca ccacacttgc aggaacaagg aagtgtatac acagaaccat
      241 cttcagttgg ttccatacag tactcaatta caattattac cagaaggggc acgaagctat
      301 ctcagatttt gacaggcaga tccaacaaac tggctccgcc acaccaggaa catctcttca
      361 ccaaagaggt gtcgaggaat agcttctggt cccttctgag aatgtaggga gatcactatg
      421 ggagtagaaa ttgcgattag tgtcactcgt gaagaaacac gggttgccgt gctcgacaat
      481 cgtgtcgtaa cagaattgta tgtcgaccgt ccaagaaaaa aagactttgt gggagacatt
      541 tacaaaggca aggtcattaa agttctgcct ggtatgcaag cggcctttgt cgatattgga
      601 ctggatcggg ccgcgtttat ccacgtatcc gatctttcgg tcggaaccga acccggggat
      661 accctcgtgg atagtgaaga ggatgaacga agtcaagacc tcccgcgtcc acgacgccaa
      721 actgcacgcc ccattgaaaa cctgttgaca gaaggccagg aacttcttgt gcaaatttcc
      781 aaaggcccca tcggtaccaa gggacctcgg attaccacat acgtctccct tcccggacga
      841 ttcttggtcc tcatgcccaa tgtcgaccat attggcgtgt cgcgaagaat tacaaaagaa
      901 gaagagcgcc atcgtttgaa ggacctcatg cggcgcatcc ggaaacccga atatggatat
      961 attgtcagaa ccgtgtgtga agatgtaccg gaggacgatc tccaaaccga cgtaagattc
     1021 ctcacagcat tatgggaaga cacgctcaaa gctgccggta aacaaccagc cccgtccctc
     1081 ctgcatacgg atctctccct cccgctccgg gtggtacggg acttattcac caaaaaagtc
     1141 gaccgtctgc tagttgatgc aaaaccggat tttgaggaag tcaaagattt tgtccggcgc
     1201 tatcttccgg aacaagcctc ccgcgtacat ttttatgaca agaagcagga agggttattc
     1261 gatcacttgg ggatcgaatt ggaaattacc cgagccctaa gccggaaagt ctggctcaaa
     1321 tccggagggc acattgtcat tgaccacaca gaggccatga ctgtggtaga cgtcaatacg
     1381 ggacggtttg tggggaaacg cgaccaggaa gaaacgattt taaaaaacaa tttggaggcc
     1441 gcgaaagaga ttggctacca gataaaatta cgcggcattg gcggcattat cattattgat
     1501 ttcattgaca tggaacggga acggaaccgt gaaaaagtgt accaggctac attggacgct
     1561 ctggcggggg ataaggctca aacccgtatc tcacgtattt ccgatcttgg gctcattgaa
     1621 atttcccgtg aacgagtccg tgaagatctc ttacggacgt tatcggaaat ttgtggtgac
     1681 tgcgagggaa gagggtacac caaatctacc atgaccgttg cgtatgaaat ttttagggat
     1741 atccggcgca ttggaattac tcggggacaa ccccaacaaa ttgtggtcgg tgccaacccc
     1801 aaagtcattg agctcatttt cgaaaccgag cacagtagca ttgagcaact cgaacaggaa
     1861 ttccaacaac aaatattatt tgaggcagat ccattactgc accttgaaca atatgacatt
     1921 gttgtagtag ggaaattggc gtcacgcgcc gaatctcgga ccgcatccta ggagtctact
     1981 ctgttaactg taccatttgc ctccatgcac agaaaggaga ggagacccaa atccctcctt
     2041 caactcttga aaaatccgga acactccatc atccggagtc gaaggtggaa taatttcttg
     2101 gtagagagcc atgcggacga accatctgac ggtggtctcc agacgcatac tccctatcct
     2161 cacagagaat ctgaatgacc tcacccttac aaggatggtt ggaacttttg gcagtcaaag
     2221 gacttggccc tgtcacttac accagactca tcaaccggtt cggctcgcct gatgccattc
     2281 gctcctcgac cgcccacaca ctcgaatcaa tcggggaaat ttctccctca ttggccaggg
     2341 ctcttcatca gcctatatct accgatacac aaaatcagat tgccaaagaa ttgcaggccg
     2401 ttcaggatgg gcgattctcc atcctgacac tcgtggacgc actctacccg ccacgattaa
     2461 aaaccattac agatcctcct ccgttactct ggtatacagg ccaactccag gaccgcgatc
     2521 aacaggccct tgccatagtt ggctcaagaa aaggctccca cattggacgg gcattcacgc
     2581 gacaactgag tggcgattta gctgcactgg gctttacgat tgtgagcggc atggcaagag
     2641 gaattgatgc ggcagctcac gaaggagcac tggccacatc cgggagaacc ctggccgtgc
     2701 ttggatgtgg catcgatcaa acctatccgc ctgagcacga tccgctacga caacgcattg
     2761 aacaacatgg ggccgttctt tctgaatttc cgatgggaac ccctccacat tcttaccatt
     2821 tccctcaacg taatcggatt atcagtggac tctcacttgg tgtgatcgtc accgaagcca
     2881 cctcgcggag cgggtccctc attaccgctc gaatggcttt agaccaaaat cgtgaggtct
     2941 ttgccgtacc aggaaatgta acaaattcat tgactcgtgg ccctcaccgc ctcattaaag
     3001 agggagcgaa attagtcgaa aattctttag atgttgtaga agaaattctt cctatgttag
     3061 aaccctcctt ccgggatcaa ctcgagaaac aacaaggcgg gtttcagcct caaccgtcca
     3121 cgcctgcact aggagcagag gaacaacatc tatttgattg catttcactg gagcctgttt
     3181 ccctggacga tttaattaat caatgttcct atgccccacc tgaagtgatg agcattctct
     3241 tgtctttgga gatcaaaggg cttataaagc agataccggg actacagtat ctcaggatga
     3301 gcattggata gtacttcaac aggtcaattg tgctcgtaaa cgattcatga ttttcatcac
     3361 tgtctatgac tagatcactc gtcatggtcg aatcgcccat caaaccacta tccaaatatc
     3421 ttggacgcca taaccaagca ttggcatcgg tgggggcatc ttacatgcgc acggaagagt
     3481 aaaccgggca ttgacctcga acaccatttt gacggggctg tcgaaaagaa tagatttgta
     3541 agatacgcca ctgattttca tgttggacca agttaaaaaa cccctagtaa aattcgcaac
     3601 ttttatccgt cagacacgga gttacaaccc ctcatcgcca tcaaaaacat tttttgcgac
     3661 agaaccgtcg gcaggctagc cttggcggct gactgaaacg gctgcgcccg ctggaaaaaa
     3721 tgacttgaac a
//
LOCUS       DAORAQ010000248         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3493_length_3697_cov_75.679023, whole genome shotgun sequence.
ACCESSION   DAORAQ010000248 DAORAQ010000000
VERSION     DAORAQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_3697_cov_75.679023"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKM72_13595"
     CDS             <1..550
                     /locus_tag="PKM72_13595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP61874.1"
                     /translation="GLVENLLDMSKVEAGTLVPHREWHLLEDLVEGAIRRVEMSLESR
                     SLEVNLGEKISPIFVDGIEIQQVLINLLDNATKYSPAGSLVSIVGRMMPNQVEVRVSS
                     EGEGIPQEELVSIFDRFYRLKGPRTRLIRGTGLGLAICKGMIEAHGGRIWAESQNRVV
                     TICFTVPTPEAPTMTIFDREEE"
     gene            562..1275
                     /locus_tag="PKM72_13600"
     CDS             562..1275
                     /locus_tag="PKM72_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNP61875.1"
                     /translation="MAPGSRILAVDDERQIRRSLQINLEAKGYEVLTAETGEEALQIM
                     SHRLPDLALVDLLLPGMDGIELTRHLCETYQIPIIILSAIGEEAKKIEALEVGADDYV
                     TKPFSMEELTARIRSVLRRSSSISGNDPVFTFGNLQINFESRNVCIRNAPVKLTPTEY
                     DLLKFLIQNSGKVLTHGTILRAIWGAGYSEQAQYLRVFIGNLRKKLEKNTARPQYILT
                     DPGVGYRFATEFVEDQKPV"
     gene            1711..2160
                     /locus_tag="PKM72_13605"
     CDS             1711..2160
                     /locus_tag="PKM72_13605"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNP61876.1"
                     /translation="MKILNGTTFATTLIMVGCPLLLFSTLNPVWASTQPPEVKVSMDY
                     WAPYYSPALAIVPDGASIHVVNPTSSPHSLTHDGCRGTGSCAFDTGAIQPGQEFTIHS
                     LPPGRYSYYCVLHPIMKGEIIVIPDHTVINQGATDDYRTATHAKKTQ"
     gene            2254..2619
                     /locus_tag="PKM72_13610"
     CDS             2254..2619
                     /locus_tag="PKM72_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61877.1"
                     /translation="MKSQKCVLIVDDDADVRLFLRDRLNAMGFEVLTAANGKEGVETL
                     RHHSVDGVLLDLYMPVMGGILMLEQLAQFSVFPPVIVMSSTTHRSELQFAIIKGAMDF
                     LIKPIAPEELTDKCMRLFY"
     gene            2663..3562
                     /locus_tag="PKM72_13615"
     CDS             2663..3562
                     /locus_tag="PKM72_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61878.1"
                     /translation="MKKGKLNIAPGLPTADISRIQESFGMLAAHAEQMVSRFYNVLFD
                     KFPEFQTFFPQSQLSQQHAAFLRGLHTLVLGIENPQELRSTLVQLGERHQRYGIKNKH
                     YPPVVYALMHVLTEFGGDGIDGRTYDAWENFLHLMRAIMLESYSRERVTPEDQKEKSL
                     TTVAGGHTKRILLIDDDHQLLELYQSYLELEGYLCSRVSEVAWAFTHIQMSHYDLVLT
                     DFQMPVMNGIQLRRNLEYVGNGCCPPFVLVTGSLNQEIRRQALDSGFEAVLKKPHDLS
                     ELGSLIGKVLKKSNGYQGNGQNL"
BASE COUNT          945 a          802 c          914 g         1036 t
ORIGIN      
        1 tggattggtt gaaaatcttc tggacatgtc aaaggttgaa gcgggaacac tggttcccca
       61 tcgtgaatgg catttattgg aagatttggt cgaaggtgca atcaggcgag tggaaatgtc
      121 attggaaagc cgatctcttg aagtgaacct tggggaaaaa atctctccta tttttgtgga
      181 tgggatagaa attcagcaag tcctgatcaa cctgttagat aatgccacta aatattctcc
      241 tgcaggatcc ttggtcagca ttgttgggcg aatgatgccg aaccaggtgg aggtacgggt
      301 ctccagcgaa ggagaaggaa tccctcagga agaattagtc agtattttcg atcgatttta
      361 ccgactaaag ggtccgcgga cacgactcat tcgtggtaca ggtttgggat tggcgatctg
      421 taaaggtatg atcgaagcac atggtggtcg catttgggcc gaatctcaga atcgagtcgt
      481 gacgatttgc tttacggtgc cgactcctga ggcacccacg atgaccattt ttgaccgaga
      541 agaggagtaa gcgccctacc tatggctcct ggatcccgaa ttttagctgt tgacgatgag
      601 cggcaaatac gtcgttcctt gcagatcaat cttgaggcaa agggatatga ggtcttgacg
      661 gctgaaaccg gagaagaggc cctccaaatt atgagccacc gccttccgga cctggctctg
      721 gttgatttgc tgcttcccgg aatggatgga atcgaattaa cccggcattt gtgtgaaacc
      781 tatcaaatcc cgatcattat tctttccgcg attggggaag aagcgaaaaa aattgaagct
      841 cttgaagtag gtgccgatga ctatgtgacc aaaccgttta gtatggaaga gctgacggcg
      901 agaatccggt cagtcctgcg acgttcttcc tcaatttctg gtaacgatcc tgtgttcaca
      961 tttgggaatt tacagatcaa ctttgaaagt cgtaatgtct gcattcgaaa cgcgcccgtc
     1021 aagttaaccc cgacggaata tgatctgttg aagttcctca ttcagaattc cggaaaagtt
     1081 ttgacgcatg gaaccattct ccgggcgatt tggggggctg gatatagtga acaggctcaa
     1141 tatttacgtg tcttcattgg aaacttaaga aaaaaattgg aaaaaaacac ggcccgtcct
     1201 cagtatattc tcacggatcc cggagtcggc tatcgatttg ccaccgaatt tgtggaagat
     1261 cagaaacccg tctgactttc cgttttcatg tgcggtgttc cttatccgac atggtccctt
     1321 gctattccat ctcctttaac agccatccct tcttttcgtt cctcttgtaa ttctccacct
     1381 ttttccacgt cgtctttttt gactttcctt tgtaccagtt cccttcaagg gagcgggttt
     1441 gcagaccttg aattgctcgt gctgatgggg atcatggcag tttgactctc tccggtcatg
     1501 gtttttgcaa ttttcgtcaa gcgtcattag aaggaatcct aatcctccca acgaccggat
     1561 ggtctctcca ggtgtcagaa agagaggcca tgaaatcttt atgaaatctt tattggaacg
     1621 gaatgcaaat caaatagatt ctttatggtt ttggaaatta ccatttaaat gaaggctttc
     1681 gaggagcttg agaaatcgcg aggtgaacag atgaaaattt tgaacggaac caccttcgct
     1741 acgacactaa ttatggttgg atgccctctt ctattgtttt cgaccttgaa tcccgtttgg
     1801 gcttccacgc agcctccaga ggtgaaggtc tcaatggatt attgggcgcc gtattatagc
     1861 ccagctttgg ccattgtccc tgatggagct tcgattcacg tcgtgaatcc tacttcctct
     1921 cctcacagtt tgacgcacga cggttgtcga ggaactgggt cctgcgcatt cgataccggt
     1981 gccattcaac ccgggcagga attcaccatt cattcactcc cgcccggtcg gtattcctat
     2041 tattgcgtac tccatcccat tatgaagggg gaaattatcg tgattcctga tcacacggtt
     2101 attaatcaag gtgcgactga cgattatcgg acggccactc acgccaagaa aactcagtaa
     2161 gggattggtg gtggtcaatc attccatact cacccgttta ttcgggccgc gggacgccaa
     2221 tgaatgaatg atttctcaag ctgctcatta ccgatgaaaa gccaaaaatg cgttcttatt
     2281 gtcgacgatg atgcggacgt ccgtttattt cttagggatc gcttgaatgc catgggtttt
     2341 gaggttctca ctgcggctaa tgggaaggaa ggggttgaga ctcttcgcca tcactccgta
     2401 gatggcgtct tgctcgattt atatatgccc gtcatgggag gcattcttat gctggaacag
     2461 ttagcacagt tttcagtttt tccgccggtg atcgtgatgt cgagcaccac acaccgatcg
     2521 gaactgcagt tcgctattat taagggagcg atggactttc tcatcaaacc gatagctcct
     2581 gaggaattaa cggataaatg catgcgactc ttttactagt cctgtaaagc caagaaggaa
     2641 tcttatcctc ggaaaggaaa cgatgaagaa aggtaaatta aacattgctc cgggacttcc
     2701 cactgctgat atatcacgga tccaagaaag ttttgggatg ttagccgctc acgccgagca
     2761 gatggtttct cgattttata acgtgttgtt tgataaattt cccgaatttc agaccttttt
     2821 ccctcagagt cagttatcgc aacaacatgc ggcatttcta aggggattgc acactctcgt
     2881 tttgggcata gaaaaccctc aggaattacg ttctacgctc gttcagttag gggaacgcca
     2941 tcaaaggtat ggaattaaaa acaaacatta ccctcctgtt gtttatgccc tgatgcatgt
     3001 cctgacggaa ttcggggggg atggcataga tggaagaaca tatgacgcat gggaaaattt
     3061 ccttcacctg atgagagcta ttatgcttga aagctactct cgggagaggg tgactccgga
     3121 agatcagaaa gagaagtccc tcaccactgt agctggaggt cacacaaagc gaattttgct
     3181 cattgatgat gaccaccaac ttttggaact ttatcaatca tacttggagc ttgaagggta
     3241 tttatgcagt cgggtttcgg aagtggcttg ggcatttacc catattcaaa tgagtcacta
     3301 tgacctcgtc ctgacggatt tccaaatgcc ggtgatgaac gggatccagt tacggagaaa
     3361 tcttgaatat gtggggaatg ggtgctgtcc gccatttgtc ttggtaacag ggagtctcaa
     3421 tcaggagatt cgaagacaag cgttggactc cggattcgag gcggtgctta aaaaaccaca
     3481 tgaccttagt gaattgggtt ctctcatagg aaaagtctta aagaaatcta acgggtatca
     3541 aggaaatgga cagaatctgt gaagcgggat gtaggaaaat gcggaagtgg tgtttgctga
     3601 aattaaccag ctaaataagc tgtctggacg gaaatttttt tagagggatt tgttctccat
     3661 gttcccctgt catatcggat ttcgtggtgc cgtgatt
//
LOCUS       DAORAQ010000249         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3509_length_3685_cov_69.226722, whole genome shotgun sequence.
ACCESSION   DAORAQ010000249 DAORAQ010000000
VERSION     DAORAQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_3685_cov_69.226722"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PKM72_13620"
     CDS             complement(<1..719)
                     /locus_tag="PKM72_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP61879.1"
                     /translation="MNERVALITGGARGIGRGIALDLAAKDWKVAICYRTSQLEAQDV
                     VRTIEEKGGKALAIQCDVSDAIAAVQLVQQVENTWDRIDALINGAGPYHRVPLFDETT
                     DGWSEMFNNNLHPIFYLAKAVAPGMKARQSGRIISFGMANADKMEAQSHVTAHYIAKA
                     GVLILTRTLAKILAPDGITVNAVSPGFIDSGSAPPGELDGIAKKIPAGYIGEIQDTVQ
                     AVRYFLSDEARYVTGANLHVSG"
     gene            complement(780..1553)
                     /locus_tag="PKM72_13625"
     CDS             complement(780..1553)
                     /locus_tag="PKM72_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNP61880.1"
                     /translation="MTPSGNDPARRRSRPSPQGQGKGHRSLPPAKSAIIVFAKAPVAG
                     QVKTRLCPPLTPDEAASLHGSLVLDILERCQSLKEYDRILAGTPSHHHPFFRAMEARF
                     KIPVWDQQGEDLGERMAGVFKQALGAPYRSAVVIGTDIPGIHGPLLKSAVKGLEDHDV
                     VLGPTGDGGYYLIGLRSPVPDLFENMPWSTDQVFANTKQRVQELGLSLKVLPPLRDLD
                     TVEDLQLFIREAKDRQNQTFSSRTKNVLQELAARLTNRE"
     gene            complement(1537..2238)
                     /locus_tag="PKM72_13630"
     CDS             complement(1537..2238)
                     /locus_tag="PKM72_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNP61881.1"
                     /translation="MDIAVIIPVLNEQKALAVLLPELLSSFGFEELIIVDGGSWDQTT
                     DVARTICQSAPNVHTQIINGPRGRASQMNAGAALAKSDILVFLHADTQLPRNARQVVT
                     QAMDDQGCVGGRFDVRFPQDTGYAWMVSRLMNLRSRWSGISTGDQTMFVRRSVFEELG
                     GFANIPLMEDIEFSRRLKRVGPIAALRAKVVTSFRRWDQHGPLRTIVRMWILRAWYWL
                     GWDPRRLQQYYDTVR"
     gene            complement(2293..2724)
                     /locus_tag="PKM72_13635"
     CDS             complement(2293..2724)
                     /locus_tag="PKM72_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61882.1"
                     /translation="MSTETDPPQSTDPVVPKPVPPRPPVMEEDQEDQENLQMQVAMRM
                     VGSAMVFIGFLQVFLSASTGAEISIFPMIIYFSGMAMWAYSSVDNLTVRYTVMALSLL
                     CAMAFMHFGEVLFWHKYLIYWGTIGVVVFFMFKNPKKPADS"
     gene            complement(2727..>3685)
                     /locus_tag="PKM72_13640"
     CDS             complement(2727..>3685)
                     /locus_tag="PKM72_13640"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HNP61883.1"
                     /translation="EDLYRSDMVMYDMDLTNGAILEKGHVYLVPLMERVKLPKDIRGR
                     TNPKSSTGRLDIFTRVVTDLHVGFDEIRSGYEGPLFLEIVPRSFTIRVQAGLALNQLR
                     LMSGKPMVSDSGLRTVHRKTPLLFHNGDSGKTDLPLSGKELRMNNGLFLSVDLQGSSD
                     HSEAIVGYRAKKNSHVIDLSLVGHYAATDFWEPLKRNATATMLLEPEEFYILASKERI
                     QVPPGYSSEMVAYEAACGELRTHYAGFFDPGFGYANPNRKGTQVVLEVRPHDVPFRIH
                     DGQTFFKVVYEKMRDIPMQLYGSALGSSYFQQGLTLSKHFKW"
BASE COUNT          937 a         1041 c          875 g          832 t
ORIGIN      
        1 ccgctcacgt gcaggttggc tcccgtcacg tatcgagcct cgtcagatag gaaataacga
       61 acggcttgaa cggtatcttg gatttccccg atataaccgg ccggaatttt ttttgctatc
      121 ccgtcaagtt ctccaggtgg tgcgctccct gaatcaataa atcctggtga aaccgcattc
      181 acggtaatac catcgggagc gaggattttc gccaacgtcc tggtaagaat gagcacgccg
      241 gccttggcaa tataatgggc ggtcacatgc gattgggctt ccattttgtc cgcgttggcc
      301 atgccgaacg aaatgatccg tccggattgc ctagccttca tccctggggc gacagccttc
      361 gcgagataga agatcgggtg aagattattg ttgaacatct ccgaccagcc atcggtggtt
      421 tcatcaaaaa gcggcacccg atggtaaggt cccgccccgt tgatcagcgc atcaatccgg
      481 tcccatgtat tttccacttg ctgaacaagc tggacagcag caatggcatc tgagacatca
      541 cactgaatgg ccaaggcctt tccgcctttc tcttcaatgg tgcgcacgac atcctgcgcc
      601 tccaactgac tggtgcgata acaaatcgcc accttccaat ccttggcggc taagtccagg
      661 gcgataccac gcccaattcc ccttgctcca ccagtgatca acgccacccg ctcgttcatg
      721 gcttccaacc cctgttcttc tcataatcac acaccagtcg cacaggaatc atttccccat
      781 tattcacggt tggtcaatcg tgctgctaat tcttgcaaca cgtttttcgt acgagaagaa
      841 aatgtttgat tctgccggtc cttggcttcc cggataaata gttgaagatc ctcaaccgta
      901 tcgagatctc gtagaggcgg taaaaccttc aaagataaac ccaactcctg aactctctgt
      961 ttggtattcg caaacacctg atcagtggac catggcatat tctcaaacag gtctggaaca
     1021 ggtgatcgca gaccgattaa ataatatccg ccatcccctg tggggcccag caccacatca
     1081 tgatcctcca agcccttcac ggctgatttc agcagtggtc catgaatccc gggaatgtcg
     1141 gtgccaataa ccacagccga acgatagggt gctccaagcg cctgtttaaa aaccccggcc
     1201 atgcgctctc ccaggtcctc cccctgttga tcccagacag ggattttgaa tcgagcctcc
     1261 atggcccgaa agaatggatg atggtgagag ggcgttccgg ccagaatccg atcgtattct
     1321 ttgagagatt gacatcgctc taagatatcc aagacgagac ttccgtggag actggctgcc
     1381 tcatcaggcg tgagaggagg acagagacgt gttttgacct gcccggcaac cggtgccttc
     1441 gcaaagacaa taatcgccga cttcgcaggc gggaggcttc gatgtccttt cccctggccc
     1501 tgcggtgatg ggcgtgatcg acgtcgagct gggtcgttac cggacggtgt cataatactg
     1561 ctgaagacga cgaggatccc atcctagcca ataccacgcg cggagtatcc acatgcgcac
     1621 gatcgtgcgg agtgggccgt gctggtccca ccgtcgaaac gaggtgacga ctttagctcg
     1681 cagagccgcg atcggaccaa ctcgcttcaa ccggcggctg aattcaatat cttccatcag
     1741 aggaatattt gcaaagcctc ccaactcttc aaacactgaa cgtcgcacaa acatggtttg
     1801 atcgccggtg gaaatccccg accagcgtga gcgaagattc atgagccggc tcaccatcca
     1861 tgcataaccg gtgtcctgtg ggaaacgtac atcaaaccgg ccaccaacac acccctgatc
     1921 gtccatagcc tgtgtcacca cttgtctggc attgcggggc aattgagtgt ctgcatgcaa
     1981 aaacaccaga atgtccgatt tggccagagc ggcccctgca ttcatttggg aggcccgacc
     2041 acgtggcccg ttgataattt gagtgtggac attaggtgcc gattgacaga tcgtccttgc
     2101 cacatccgtc gtttgatccc aactcccgcc atccacaatt atcagctcct caaatccgaa
     2161 tgatgacagc aattcaggca acagcacagc caatgccttc tgttcattca agaccggaat
     2221 gatcacggcg atgtccatga agaccctaaa tgtcggcgca cacaggaaag ataaaaaacc
     2281 aagaacgtac gtctacgagt cggcgggttt cttgggattt ttgaacataa aaaacacgac
     2341 gactccgatc gtgccccagt aaattagata cttatgccaa aataagactt ccccaaaatg
     2401 cataaatgcc atcgcacaaa gcagagacaa cgccatcacc gtataacgga ccgtgagatt
     2461 gtccacggag gaataggccc acatcgccat gcctgaaaaa taaataatca tgggaaagat
     2521 gctgatttcc gcaccggtac tggcggacaa aaaaacctgc aaaaacccga taaagaccat
     2581 agcggatccc accatacgca tggccacctg catctgcaga ttttcttgat cctcctgatc
     2641 ctcctccata actggcggac ggggaggcac gggcttggga acaacaggat cagtcgactg
     2701 aggtggatcg gtttctgtac tcataattac cacttaaaat gtttgctgag tgtgagaccc
     2761 tgctggaaat aggatgaccc caaagccgat ccatataact gcatgggaat atcacgcatc
     2821 ttttcgtaaa caaccttaaa aaatgtctgt ccgtcatgaa tacggaaggg cacatcgtga
     2881 gggcgaacct ccaaaaccac ctgggtgcct ttccggtttg ggttggcata gccaaagccc
     2941 ggatcgaaga acccggcata atgggttcgt aattccccgc aggcggcttc atacgccacc
     3001 atttccgatg aatacccagg tggaacctgg atacgttcct tggaggccag gatataaaac
     3061 tcttccggct ccaatagcat cgtagccgtg gcattgcgtt tgagcggttc ccaaaaatcg
     3121 gtcgcggcat agtggcccac cagactgaga tcaatgacat gactgttctt tttggcccgg
     3181 taccccacaa tcgcctcgga atggtcactc gatccttgca gatcgacact taaaaacaga
     3241 ccgttattca tccggagttc cttgcctgat aggggcagat ccgtttttcc cgaatctcca
     3301 ttatgaaaca gcaagggagt cttgcgatga acggtccgca accctgaatc cgagaccatg
     3361 ggctttcctg acattaatcg caattgattg agagccaatc cagcctgcac tcgtatcgta
     3421 aacgaccgcg gcacgatttc caaaaataac gggccttcat accccgagcg gatttcatcg
     3481 aagcccacat ggagatccgt caccacgcgg gtaaaaatat ccaaccttcc cgtggaactt
     3541 tttggattgg ttcgaccgcg gatatctttg ggcagcttca cccgctccat caaggggacg
     3601 aggtagacat ggcctttttc cagaatcgcc ccattggtca gatccatatc gtacatcacc
     3661 atatcggacc ggtagaggtc ctcaa
//
LOCUS       DAORAQ010000250         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3514_length_3683_cov_62.761301, whole genome shotgun sequence.
ACCESSION   DAORAQ010000250 DAORAQ010000000
VERSION     DAORAQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3514_length_3683_cov_62.761301"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..757)
                     /locus_tag="PKM72_13645"
     CDS             complement(8..757)
                     /locus_tag="PKM72_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNP61884.1"
                     /translation="MTNILFDFTITQLLLVGVLFIWTGFVRSGLGFGGAALGLPLMLF
                     LYDQPLFWLPVIGTHLLFFSSLTLRTGIHDVDWPYLRHATLFVIPTTLIGVFGLITLP
                     TAWLLIFIYSISLAYGLMWLLNLSINSRKSWSDVLLLALGGYVAGTSLTGGPLMVAVT
                     MKNVATNQLRNTLYVLWFTIVTIKMSTFVILGVELHLLTALVLLPIAAIGHVIGLKFH
                     DAILKNDQWFKQVIGGMLVLISGLGLMNNLA"
     gene            complement(1175..1420)
                     /locus_tag="PKM72_13650"
     CDS             complement(1175..1420)
                     /locus_tag="PKM72_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNP61885.1"
                     /translation="MAWTVYMLECADTSLYTGITVDLARRLKEHSKGKGAKYTRRRGP
                     FTVVFTETQETRGQALIREAAIKAMTREGKLGLLGTG"
     gene            complement(1537..1902)
                     /locus_tag="PKM72_13655"
     CDS             complement(1537..1902)
                     /locus_tag="PKM72_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61886.1"
                     /translation="MKLTVGQGGLAQRSDPDEYDVSAIPPFKTLLNFLNSTHVYVGTA
                     AIAAMLLHIPIVGLHADTLFFPAVLAMVLWQGVFWFFLTCRFSPQELKKVSYVVHAQF
                     VTGIMIGIFSGLGHPLLDD"
     gene            complement(2374..2895)
                     /locus_tag="PKM72_13660"
     CDS             complement(2374..2895)
                     /locus_tag="PKM72_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61887.1"
                     /translation="MAKAVFCIATTFLQAEEIVDKLKNAGFASTDISVLFPDTHGAKD
                     LAHEKHTKAPEGAVTGAGAGGVLGGALGWLVGIGALALPGIGPFVAAGPIMAALGGVA
                     VGATVGGLTGGLVGMGIPEYEAKLYEGKIKEGKILISVHSENPDTTDCAKTIFKNENA
                     EDIAISDEAAVKS"
     gene            complement(2930..3346)
                     /locus_tag="PKM72_13665"
     CDS             complement(2930..3346)
                     /locus_tag="PKM72_13665"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNP61888.1"
                     /translation="MKHVKGCLMILACVGLAGLGDVTFVSGHTLPGSVVVAVDSVEDS
                     EKNLRDNDSNVLTPFDQSKGSEEDVEMTRRIRQALMADTTLSMNAHNIKIVTLNGKAT
                     LRGPVDTQDERRRILKIATLIVGLNNVINLLEVEND"
BASE COUNT          956 a         1007 c          863 g          857 t
ORIGIN      
        1 ggctgtctta ggccagattg ttcattaatc ccaatccgct gatgaggacg agcatgcctc
       61 ctatgacctg cttgaaccac tgatcgtttt tcaaaatcgc atcatgaaat ttgagtccga
      121 tcacatggcc gatggctgcg atggggagga gtacaagggc ggtgagcaga tgtagctcga
      181 cgccgagaat cacgaaggta ctcattttaa tcgtgacaat ggtaaaccag agaacgtaca
      241 aggtgttacg tagttgattg gtcgcgacat ttttcatggt gaccgcaacc atcagtggtc
      301 caccggtcag tgacgttccc gccacatacc caccaagcgc aagcaacagc acgtcgctcc
      361 agctcttcct gctattgatg gaaagattca atagccacat caatccatac gcgagtgaga
      421 tggaataaat aaagatgagc agccacgcag tcggcaacgt aatcaggccg aacacaccaa
      481 tcaaggtcgt ggggatgaca aaaagcgtgg cgtgccgcaa ataaggccaa tcgacatcgt
      541 gaataccggt acgcaaggtc agagaagaaa aaaacaggag atgcgttccg atgaccggca
      601 accaaaatag tggttggtca tagagaaaca gcatgagtgg gagtccaagc gcagcaccac
      661 cgaacccaag cccgctgcgt acaaaccctg tccagataaa cagaactccc acgaggagta
      721 gctgagtgat cgtgaagtca aagagtatgt tcgtcatgga tcgtgcgttt actgaaaaaa
      781 gccgccaacg gccttccctg ctttcacgtt cgcccctgaa gccagctttg gcgcgcaagc
      841 gcgcaggtcg gtcgcaaatt ttgaatgccc tcgtactatc ccaaatacgg gtcaagtcag
      901 tttatacagg ttatatcatg caacacatca cgaattttcc ctgcagaact tctcaacgta
      961 ccatttccct cacagtcaga tctgttttaa agaattatct atcccctaca ggcaaagatg
     1021 gtcacataaa tttgaggtat tacacctctg aggggtccga ccgagaagtg tagatggatt
     1081 cacacttact tctggcggaa atgactgagg cgaaaaagat taggaatgcg gaatgaagag
     1141 tagaagaggg agggtcctcg tccaagcgat ccttttatcc agttccgagt aagcccaatt
     1201 ttccttctct ggtcatggct ttgattgccg cttctcttat gagcgcctgg cctctcgtct
     1261 cttgagtttc ggtgaacacc accgtgaacg gccccctgcg tctggtatat ttggccccct
     1321 tacctttcga atgctcctta agacgacgcg ccagatccac ggtgatcccc gtgtacaaac
     1381 tggtatcggc gcattccaac atatacactg tccaagccat caacaccctc ccccttctct
     1441 ttttgctgcc cccgtttgcc tgtgagtgac atgaacccta cccattcgag gccacaggga
     1501 tctcacctga caaatcgtgc tcttccccct gaacgactaa tcgtcaagca gcggatgccc
     1561 caaccccgaa aagatgccga tcatgattcc ggtcacaaat tgggcatgca cgacatagga
     1621 aacctttttc aactcctgag gagaaaatcg gcatgtgagg aaaaaccaaa acacaccttg
     1681 ccataacacc atggccagaa cggctgggaa aaataaagta tctgcatgca ggccgactat
     1741 cgggatgtgc aatagcatgg ccgcaatggc ggcagtcccc acatagacat gagtgctatt
     1801 taaaaaattg agaagggttt tgaatggggg gatcgccgac acgtcatact cgtccgggtc
     1861 acttcgttgc gcaagcccac cctgaccaac cgtcaatttc accaatgttc ttccataaat
     1921 aataaccagg acccagacgc cgagggtccc gaagacatcc cccatttcgc gcagaaacgt
     1981 ttcgtgctcg ggattctcag gtcgattcgg atagatggag aggaagtttc ggacaaagga
     2041 cccaaaacca tattcacctc ggtcgggctg atggaatcga gctcccgtag atcccagggg
     2101 aggcggccag actcgccagg ctcaaacaac ggccgcccac tgttgagagc ggcagcatgg
     2161 gtgatcgacc tgaaggtggt ggagagagga atttatttat tttttctgct acccgattag
     2221 gaacgtcgag aataatagtc tggacttcct ttgggcggac tgcaggaacc tgtcccgcca
     2281 tttccatggg aaaaatgcgc tccgaggaca agagggtcaa aaggtttccg gcacatcttc
     2341 cgcctaacta cccgtttcgg tccttacccg tttttatgat ttgactgcgg cttcgtccga
     2401 aatcgcaata tcctctgcat tctcattttt aaagatcgtt ttggcacaat ccgtagtatc
     2461 tggattttct gaatgcaccg agatcagaat tttgccttct ttaatcttcc cttcatagag
     2521 cttggcttca tactccggga tacccatacc gacaagcccg ccggttaggc ctcctacggt
     2581 agctcccact gccactcctc ccaacgcagc cataattgga ccggcagcca caaacggacc
     2641 tatgcccggc agcgccagtg ccccgatccc caccaaccaa ccaagagccc cgcctaacac
     2701 ccccccggct ccggctcccg tcacagcccc ctccggagcc ttcgtgtgct tttcgtgtgc
     2761 taaatccttg gccccatggg tgtccggaaa aagcacagag atatccgtac tcgcaaatcc
     2821 tgcatttttg agtttgtcga caatttcctc ggcttgcaaa aaagttgtcg caatacaaaa
     2881 tacggcttta gccatcgcgc actccttttc cgggggataa agaggttatt taatcgtttt
     2941 ccacttcaag cagatttatg acattgttca ggccaacaat taacgtggcg attttcaaaa
     3001 tccgccttcg ctcatcctga gtatccaccg gtccgcgtag ggtggctttt ccattcaacg
     3061 tgacaatttt aatattatgg gcattcatgg ataaggtggt atcggccata agtgcctgcc
     3121 gaatacgccg ggtcatttcc acatcctctt cactgccttt ggactggtcg aatggagtca
     3181 atacattgct atcattgtcc cgaaggtttt tttccgaatc ctcaaccgag tctacggcga
     3241 caacgacgga gcccgggagt gtatgaccgg aaacgaaggt gacatcccct agccctgcca
     3301 gaccaacaca ggccagaatc atcagacaac cttttacatg cttcatcctg tctccgccat
     3361 aaggttagtg tagtcgcaat caagggagat gagccgcctc cgatcagatt gatgaacgtg
     3421 accgtcgttt ccctttgctc ggtgggcata ccttacccaa acctacctaa aaatgccacc
     3481 ctttccgggg cataaaacac cgatgcgtag agtcacgatg gataaccgct gaggaaggga
     3541 aagccctgaa aattcggcct agctggcaaa ggaaaatgca aggatattgt cgtccacaaa
     3601 gatctttcgg tgaaggtatc aatgacaggg tatcatcctt gcccattccc tcttgccctg
     3661 tgtgccggaa agaggtctca atc
//
LOCUS       DAORAQ010000251         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3539_length_3665_cov_57.544044, whole genome shotgun sequence.
ACCESSION   DAORAQ010000251 DAORAQ010000000
VERSION     DAORAQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3539_length_3665_cov_57.544044"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKM72_13670"
     CDS             complement(<1..920)
                     /locus_tag="PKM72_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNP61889.1"
                     /translation="MRDDQKFLWNTAEPGMGDHKSAWSRRPREWWALAKEVWAASCED
                     DVFGRAAQLGFYFLLALFPAMLGVTALIGMLPRQVVVPNVMPYAREILPAESLILVER
                     YVDQMIQGSGGGLFSLSLLGSLWAASWGMMAIINTLNAVYGVKETRPLWKAGLTAVLL
                     TIGAAVFFITSLILILAGEEVSQWVTEVTGLEWWATIAWPLLQWPVIVLFMLMAINLV
                     YYWAPNSDHAWQWVKPGSVLAVFLWIILSLGLKFYVENFINYNAVYGPITGVIILMMW
                     LYVGGLTLLLGGELNFILKRPHIMDGSDSS"
     gene            complement(1006..2550)
                     /locus_tag="PKM72_13675"
     CDS             complement(1006..2550)
                     /locus_tag="PKM72_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNP61890.1"
                     /translation="MKPALAILGTGSDVGKSLVTAGLCRLLHRAGVRVAPFKAQNMSL
                     NSYVTPDGGEMGRAQVLQAQACGLAPHVDMNPILLKPEADAKSQVIVQGKVWRSQNAR
                     DYFDRKSELIEFVRASYDRLCKQYDVMVLEGAGSAAEMNLRDRDIVNWPMVEMADAAV
                     VLVADIDRGGVFAQVIGTMNLLAPEERQRVIGVVINKFRGDLTLFEDGVTFIEKHTGV
                     PVFGVLPYLRNLELDQEDSVDIDRFRKTLFEDDTVNIAVVLLPHMSNFTDFNRLAAEP
                     DVALRYVASPRELQGADAIVLPGSKTTIADLEYLRKVGFEEALKTHVQRGSELMGVCG
                     GFQMLGKEITDPQHVETGGSSKGFGLLEVETELLAHKKTVQVRACSLPESWGHECPVE
                     GYEIHMGATRGANDIPSCFGISPNQEKFSLEKEDVRLDGAMSPDGRVWGTYIHGVFDQ
                     PEFRRRWLNRMRIRKDLAPLDLEISAKVSQRMSQALDRWADHLKTHLDVDRIFSTLGL
                     GGSVPD"
     gene            complement(2646..>3665)
                     /locus_tag="PKM72_13680"
     CDS             complement(2646..>3665)
                     /locus_tag="PKM72_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61891.1"
                     /translation="VQIEVWDPWDYMGDTWHIQPYNPRNNINYTIEKSGLAERHPDPP
                     YLNTQPFFFTTPRQRNNRVVLQYQQRFVDKMLDYSLRYGHVLYCMDNETTGEEEWSRY
                     WATYLKHRADKDDKMIFVTEMWGNKDIIAEIHRRTFDHPEVYDFVDVSQNNHQSGQKH
                     WDQFLHVRNYLSKNPRPINLTKTYGATGQRFGTDQDGIERFWRNLLGGAASIRFHRPT
                     AGLGLHDKAVASIRGARKLESQIPLWTLEPADDLLTGHKPNHAYLSARRGSAFALYLP
                     VGGGVRIDVSSAEEPLVVRWIEIATGDWGPEQQIQGGGKIYLTSPGSGNWAAAITGQH
                     SAGTD"
BASE COUNT          901 a         1120 c          798 g          846 t
ORIGIN      
        1 tctgacgaat cagatccatc catgatgtga ggacgtttga gaatgaaatt cagctctcct
       61 cctagcaaaa gagtcaaccc gcccacatac agccacatca tcaggataat cactcccgta
      121 atgggtccat agactgcgtt gtaattgatg aaattctcca cataaaattt aagccccagg
      181 gaaagaataa tccagagaaa cacggcaagc acggatcccg gcttgaccca ctgccatgca
      241 tgatcactgt tcggcgccca ataatagacc aggttaatag ccatcagcat gaaaagaacg
      301 atgaccggcc actgcaacag tggccaggca atcgtcgccc accactccaa cccggtcacc
      361 tctgtaaccc attggctcac ctcttcaccc gctaagatca agattaacga ggtgatgaaa
      421 aagaccgcag cccctattgt cagcaacacc gccgtcaggc cggctttcca aagcgggcga
      481 gtttctttca ccccgtagac agcattgagt gtattaataa tcgccatcat gccccaggaa
      541 gccgcccaaa gagaaccaag gagactcaaa gaaaacaatc cccccccact tccctggatc
      601 atctgatcaa catagcgctc aacaaggatc aaggattcag cgggaagaat ttccctggcg
      661 tagggcataa cattaggcac gaccacctgt ctgggcaaca taccaatgag cgccgtcact
      721 cccaacatgg cgggaaataa ggccaataaa aaatagaacc cgagctgcgc agcccgccca
      781 aagacatcgt cctcgcatga ggcggcccac acctccttgg ccaatgccca ccattccctt
      841 ggccgtctgg accaggcgga tttatgatcg cccatccccg gctccgcagt attccataaa
      901 aacttctgat cgtccctcat ccgtatttct tacaccccct cgtatatgat caaaacatgg
      961 caggctcctg gaaaattcga ggcctcttca tttcaatttt tggtttcagt ctgggacgga
     1021 accacctaat cccaatgtcg agaaaatacg atccacatca aggtgggttt ttagatggtc
     1081 agcccaacga tccaatgcct gcgacattcg ctgggaaacc ttcgctgata tttccaaatc
     1141 aagcggcgcg aggtctttac ggattcgcat tcgatttaac catcgacggc gaaattccgg
     1201 ttgatcaaaa accccatgaa tgtaagtccc ccagacacgc ccgtccggac tcatcgcccc
     1261 gtccaatcgt acgtcttcct tttccaagga aaatttctcc tggttaggag aaatgccaaa
     1321 gcaggatgga atgtcgttcg cacccctcgt ggcccccata tgaatttcat acccctcaac
     1381 cggacattca tgcccccagg actccggtaa ggagcaggca cggacttgca ccgttttctt
     1441 gtgagcaagc aattccgtct cgacttccag taacccgaat cctttcgacg agccacccgt
     1501 ttcaacatgc tgtggatccg tgatctcttt ccctagcatc tggaatccac cacatacacc
     1561 cataagctcg cttccacgtt ggacatgtgt cttaagcgct tcttcgaatc caaccttccg
     1621 gagatactcc aaatcggcaa tcgtcgtttt acttcccggc agaacaatag catccgcccc
     1681 ctgaagttcc cggggtgaag ccacataacg aagcgccaca tccggttccg cagccagtcg
     1741 attgaaatcc gtaaagttac tcatatgtgg cagcaacacg acggcgatat tgacggtatc
     1801 gtcttcaaaa agcgtcttac ggaatcgatc aatatccacg ctgtcctcct gatccaattc
     1861 cagattccgc aaatacggta atacgccgaa caccgggact cccgtgtgtt tttctataaa
     1921 tgtgactcca tcttcaaaca acgtcagatc cccccgaaac ttattaatga cgaccccgat
     1981 cacccgctgc cgttcttccg gggctaacaa attcatggtc ccgatgacct gggcaaacac
     2041 gccaccccga tcaatatcgg ccaccaacac caccgccgca tcagccatct cgaccatcgg
     2101 ccaattcaca atatcacgat cccgcaaatt catttctgcg gcactccctg ccccttccaa
     2161 caccatcaca tcatactgct tgcatagccg gtcataactc gccctcacaa attcaatcag
     2221 ctccgatttt cgatcaaaat aatcccgggc attctgactt cgccatactt ttccttgaac
     2281 aatcacttga gatttggcgt cggcttccgg cttcaacaaa atcggattca tgtccacatg
     2341 cggagccaac ccgcaggctt gagcctgaag cacttgggct cgccccatct ctcctccatc
     2401 cggcgtgaca taggaattca acgacatgtt ctgtgcttta aagggcgcca ccctgactcc
     2461 cgcccgatgt aacagccgac acaagccggc cgtgaccaga ctttttccca catcagagcc
     2521 cgtccccaat atcgctaatg ccggtttcat gcctattgtc tccttcgaaa tagctgtata
     2581 tcttgtccca cacgtgtgcg acattgcctg gaacgatgac ctacgctcag gacacagtgt
     2641 gctgcttagt cagtccccgc tgaatgttgc ccggtgattg ccgccgccca attcccagac
     2701 ccgggagacg tcaggtaaat cttcccgccg ccttgaatct gttgctcggg tccccagtct
     2761 cctgtagcaa tttcaatcca acggaccacc aacggctctt ccgcgctgga cacatcgatt
     2821 cgcactccac cgccaaccgg taggtacaag gcgaaggcgc tgcccctgcg ggctgacaga
     2881 taggcgtgat tcggcttgtg accggtgagc aggtcgtccg cgggttccag cgtccataaa
     2941 ggaatctgcg actccagctt tctggccccg cgaatactgg ccacggcctt gtcatgtagt
     3001 cccagtcctg ccgtgggacg atggaaacga atagacgccg ccccgcccaa cagattccgc
     3061 cagaaccgtt caatcccgtc ctggtcggtg ccgaatcgct gaccggtcgc gccataggtc
     3121 tttgttaaat tgatcggtcg cggattcttg gacaggtaat tccggacatg aagaaattgg
     3181 tcccaatgct tttgtccgct ctggtgattg ttttgcgaaa catcgacgaa atcgtacacc
     3241 tccggatgat cgaacgttcg cctatgaatc tcggcgataa tatccttatt cccccacatc
     3301 tcggtgacaa agatcatttt gtcgtccttg tccgcgcgat gtttgaggta tgtcgcccag
     3361 taacggctcc attcttcttc gccagtggtt tcgttgtcca tgcaatagag cacatgaccg
     3421 tatcgcaggg aataatccag catcttatcg acaaatcgtt gctggtactg cagtaccaca
     3481 cggttgtttc gctgccttgg cgtcgtaaag aagaacggtt gtgtgttgag ataggggggg
     3541 tcgggatggc gttctgccaa tcccgatttt tcgatcgtgt agttaatatt attgcgtggg
     3601 ttatacggct gaatatgcca ggtgtcgccc atgtagtccc aagggtccca aacctcgatc
     3661 tgcac
//
LOCUS       DAORAQ010000252         3656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3549_length_3656_cov_72.656207, whole genome shotgun sequence.
ACCESSION   DAORAQ010000252 DAORAQ010000000
VERSION     DAORAQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3549_length_3656_cov_72.656207"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="smpB"
                     /locus_tag="PKM72_13685"
     CDS             complement(<1..287)
                     /gene="smpB"
                     /locus_tag="PKM72_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0070929 - trans-translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HNP61892.1"
                     /translation="MAKQKTDPSSKVLSTNRKAFYDYMIEEKLEAGIVLVGTEVKALR
                     EGRLNLKESYAAIINGKAILHNCHIGTYSHGNQMNHEPLRPRTLLLHKKEI"
     gene            657..1148
                     /locus_tag="PKM72_13690"
     CDS             657..1148
                     /locus_tag="PKM72_13690"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNP61893.1"
                     /translation="MSYFESIQSLIQDFSKGNPLGLKLSEVQLQQEWEHLVGSTMAKH
                     SYPESIRFKKLHLVADNSIWLQQLMFLKPAILEAIHSMMPELALTEVVLRIGSIPQAP
                     PQPDPISQAPPPFVGESSPFATGLAKRLSDPDLQVLLSQTITKALAEPPSSTTEPAKK
                     PGV"
     gene            complement(1117..1728)
                     /locus_tag="PKM72_13695"
     CDS             complement(1117..1728)
                     /locus_tag="PKM72_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61894.1"
                     /translation="MDFPHKAPEQSAKRKKLDPIVIMGLGILIGGIGVISLGMFLSRP
                     DRTIPPYSIGAQEGSLVAVHVPSYTSDPEIKTLIMRFGDVGRATRDFGDMKIRPTTPD
                     DPRGRYQTLQVIIFSDPFWTEPDNLHRYVANEADDASEKTFRKNFEEAVRAGYRADAE
                     GQAGWIGPWNRSGSTDRTLTMQWVFQETWEEGSSKPQASSPAL"
     gene            complement(1993..>3656)
                     /locus_tag="PKM72_13700"
     CDS             complement(1993..>3656)
                     /locus_tag="PKM72_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNP61895.1"
                     /translation="HEKRVEGISDLRDESDRDGLRVVIELKRNENASVLLNQLYKHTQ
                     MQTTFGVIMLALVGNRPEVLTLKQILAAFLEHRKEVVIRRTAYDLRKAEERAHILEGL
                     RRALEFLDEVIALIRGASSPETARHGLMSQFTLSEVQATAILEMRLQRLTQLEQDKLT
                     QEYNELVSRIAHLRTILASDAMVQQIVKQELIEIKDQYSDERRTQILPAEAELQMEDL
                     IAEEEVVVTITHAGYIKRNAVSIYRAQRRGGKGKVGMGVKEEDFVEQIFTASTHDYLL
                     FFTDAGRVYWLKVYQLPDVGRSSKGKAIVNLLTISQGERVTATLPVRVFAEDQYVVMA
                     TQKGYIKKTDLAAFSHPRQGGIIALTLEEGDRLIGVDITDGTREILLGTRMGIVIRFR
                     EEDVRPVGRTARGVRGITLAKTNEVIGMVTITSETQSSILTVTERGYGKRTQVEEYRL
                     QSRAGKGVISVKVTEKNGPAISFHQVWETDEIMLMSAEGMILRTRMGDIREIGRNAQG
                     VRLIHLPDDDRVVGVAKLAETDESDQPDPDELNGEMPDEPASSTE"
BASE COUNT          765 a         1011 c          927 g          953 t
ORIGIN      
        1 tcgatctctt tcttatgcaa cagcagggtt cttgggcgta acggctcgtg attcatttga
       61 ttgccgtgac tgtaggtgcc gatatggcag ttatgcagga tggctttgcc attgatgatg
      121 gcagcgtaac tctccttcaa attcaggcgg ccctcccgta aggctttgac ttcggtcccg
      181 accaggacga tgccggcttc gagcttttct tctatcatgt agtcatagaa ggccttccgg
      241 ttggtactga ggactttgct ggaggggtct gttttttgtt tggccatggg taattatatc
      301 ttgaagtatg ggatgatgcc gctatcggga ctgttgaaaa tggccgccag cgtcgttttc
      361 attgcttcga agggctcaac gtacctaccc ggtacgcctc gccctcctcg cgacttgcgg
      421 ccttgctgga cgaccttcct tcggcaaagc tcaggacaag ctttgaacag cccctcttta
      481 ttttatcgta aacctaacca gagtcgtctg aaataacaga atttcaatta ttcgataggc
      541 ccccgctgcc tacaagatgc cgggaaaagg atctctccct cgggtcgaga tgacaaaacg
      601 ggtataggcc caagacggat gaatcattct ggacgatacg atacaatagc tgcacgatga
      661 gttattttga gtcgatccag agccttatac aagatttttc caaaggtaat ccactcggcc
      721 tcaagctttc cgaggttcaa cttcaacagg agtgggaaca ccttgtgggt tcaacgatgg
      781 ccaagcattc ctaccctgag agtatccgct ttaaaaaact gcacctggtg gcggataact
      841 ccatttggct tcagcaactc atgtttttga aaccggctat tttggaagcg attcactcga
      901 tgatgccgga actggcctta accgaggtcg tcttacgtat cggctccatt ccacaggctc
      961 cccctcaacc ggatccgata tcccaagctc ctcctccctt tgtcggtgaa tcatcgccct
     1021 ttgccaccgg cttagctaaa cgcctgagtg accctgacct tcaagtcctt ctttctcaga
     1081 ctatcactaa agccctggct gaaccaccgt cctccactac agagccggcg aagaagcctg
     1141 gggtttagaa ctcccctcct cccaggtctc ttgaaatacc cactgcatgg taagtgttcg
     1201 atccgttgaa ccgctccggt tccagggacc gatccatccc gcctgacctt cggcatcagc
     1261 acgatagcct gctcgaacgg cctcttcaaa gttttttcga aaggtctttt ccgaagcgtc
     1321 atcggcctca ttggcgacat accgatgaag gttatccggt tccgtccaga atggatccga
     1381 aaaaatgatg acctgtaagg tttggtatcg tccgcgagga tcgtccggag tggttggacg
     1441 gattttcatg tccccaaaat cgcgggtggc ccggccgaca tcgccgaacc gcataatcag
     1501 ggtttttatt tcgggatcac tggtatagga agggacgtgg acggctacca acgacccctc
     1561 ttgggccccg atactatagg gcgggatggt gcggtctggt cttgagagga acataccgag
     1621 ggagatgaca cctattcccc cgatcaaaat gcccaatccc atgatgacga tggggtctaa
     1681 ctttttgcgt tttgctgatt gctcgggggc tttgtgaggg aaatccaacg ctacgacctt
     1741 ccaggatgca atctcttttt cctgcttcct cccgttttcc aggtgaggta gaggtagggg
     1801 agtgatgatg ggggagaaac gagcaggcga acggcagttc gatctcccat cccttgcacg
     1861 gcctcttcga atcttgcgtc cctccttacc aaggataggg attgccgtat tattgatgac
     1921 cgctcatttt ctctggtcat ctcggaacac accaacgtct tcggtttgca ggtttgagaa
     1981 ggtcgcgtga ccttattccg ttgaggaggc cggttcgtcc ggcatctcgc cattgagttc
     2041 atccggatcc ggttggtcac tttcatcggt ctctgcgagt ttggccacgc ctaccactcg
     2101 atcgtcatcg ggcaagtgga tcagccgaac cccttgggca ttgcgcccaa tttccctgat
     2161 atcccccatg cgggtacgaa ggatcatgcc ctctgcggac atgagcatga tttcatccgt
     2221 ttcccagact tgatggaacg agatggccgg tccgtttttc tcggtgactt tgacgctgat
     2281 gacgcccttg cccgcccgag attggaggcg atattcctcg acctgcgtcc gtttcccata
     2341 tccgcgttcg gtgacggtca gaattgagga ttgcgtttca ctggtaatcg tgaccatccc
     2401 gatgacctca ttagtcttcg ccaacgtgat gcccctgact ccacgggccg ttcgtcccac
     2461 cggacggaca tcttcctcac gaaagcgaat gacaataccc atgcgggtgc ccaaaagaat
     2521 ttccctcgtg ccatcggtga tatccacacc gatgagccgg tcaccttcct ccagcgtcaa
     2581 cgcaataatt ccgccttgcc ggggatggct gaatgcggcc agatcggttt ttttgatata
     2641 gcctttttgc gtggccatca ccacatactg gtcttcggca aacacccgga ccggcagagt
     2701 cgcggtgacc cgctccccct ggctgatggt caacaaattc acaatggctt tccctttgct
     2761 ggaccgtccg acatccggca actgatacac tttgagccag tagacccgac cggcatcggt
     2821 aaagaacaac aggtaatcat gggttgaggc cgtgaagatt tgttcgacga agtcctcttc
     2881 tttgaccccc atgccgactt tcccttttcc gccacggcgt tgggcccgat agatggacac
     2941 ggcatttcgc ttaatatacc cggcatgcgt aatggtgacg acgacttctt cctcggcgat
     3001 cagatcttcc atttgtaact cggcttcggc cgggagaatc tgcgttcgtc gctcatcgga
     3061 atattgatct ttgatttcta ttaattcctg tttcacgatt tgttgcacca tcgcatcaga
     3121 ggctaatatg gtgcgcaaat gggcaatgcg ggagaccagt tcgttatatt cctgcgtcag
     3181 cttgtcctgt tccagttggg tcaggcgttg caaccgcatt tccagaatgg cggtggcttg
     3241 cacctcgctt aaggtaaact ggctcatcag gccgtggcga gcggtttccg gagaagacgc
     3301 tccacgaatg agggcaatga cctcatccag gaattccaag gcacgtctta agccctctaa
     3361 gatgtgggcc cgctcttcgg ctttgcgaag gtcgtacgcg gtccggcgaa tcaccacttc
     3421 tttacgatgt tcaaggaacg cggccagaat ctgtttgagg gttaacacct caggacggtt
     3481 ccccaccaag gccagcatga tgaccccgaa ggtggtttgc atctgggtgt gtttatataa
     3541 ttgattcagt aagaccgatg cgttttcatt tctcttgagt tcgatcacca cgcggagtcc
     3601 gtcccggtcg gattcgtcgc gaagatcgga aatcccttcc acccgttttt cgtgga
//
LOCUS       DAORAQ010000253         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3585_length_3635_cov_66.494134, whole genome shotgun sequence.
ACCESSION   DAORAQ010000253 DAORAQ010000000
VERSION     DAORAQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3585_length_3635_cov_66.494134"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKM72_13705"
     CDS             complement(<1..181)
                     /locus_tag="PKM72_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61896.1"
                     /translation="MTTQTEPDHWLKNFCLLLASPKMLVMLLTGFSSGLPLLLIGSTL
                     KFWMREEGLDLTTIGF"
     gene            complement(272..832)
                     /locus_tag="PKM72_13710"
     CDS             complement(272..832)
                     /locus_tag="PKM72_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNP61897.1"
                     /translation="MDTPLGKGVFLIATPALRDPNFRQAVVLLCEHGPDGALGVVVNR
                     PTEMNIAEVLPQVPVLEGQEHRVYAGGPVQKNSLLVLYRLNEEIEDTHAVLDGVYLGG
                     NMETLERILEVPGEHESFRAYMGYSGWGPGQLESEMESGSWLTMPARPHLVFDEDSQD
                     LWGEVLRSFGDEYTMYAHMPVDPNLN"
     gene            complement(1059..1331)
                     /locus_tag="PKM72_13715"
     CDS             complement(1059..1331)
                     /locus_tag="PKM72_13715"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HNP61898.1"
                     /translation="MAQIHCRKCDKDADPITDNLFMGKLEEEIKQKVCQTCWNEWAGP
                     GGTKTMVINEYQLNLGDENARQTLKTQMRTFLKLDDATGEFKDYRH"
     gene            1576..2511
                     /locus_tag="PKM72_13720"
     CDS             1576..2511
                     /locus_tag="PKM72_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNP61899.1"
                     /translation="MLLAGIDIGTLTCRLLVAEVNPPGEFTVVDADRRILRLGEGVDQ
                     HKRLSQAAMDRVVSTLIDWKEKTTKYPLDGVVVVATSAVRESENRKDFLARVTQETGL
                     EVEILTGDEEARRTLLGIRFGLPPAIANFFGLDIGGGSTECILAHSGMAPAVISLDLG
                     VVRLLERVLRLDPPTIQEIHQAEACIDEELAKVSKAFGTFSGIPLVGTAGSVTTLAAM
                     SQGLPRYESARVHNYELTLSSIKGLEQDLTTKTGPQRLAMPGLEPGREYVIVAGTVIL
                     RRVMETFGFDRCLVSDFGLREGILVDKAGKLKNLG"
     gene            complement(2530..>3635)
                     /locus_tag="PKM72_13725"
     CDS             complement(2530..>3635)
                     /locus_tag="PKM72_13725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP61900.1"
                     /translation="WHQNVQAETEDHLSKFHVQGIQGPHGHFEIIETTVSHLFREPCE
                     AMNGLTYGRLLGKTLRQMGWLSPKPKRIVEVGAGLGYVSKELARELSAEERKDIQYTF
                     LDLTGPFLGSQTSLARQAGWTATAIQANAERMPLADRSVDLLIDNENLADMTPIQFTG
                     DELLTFKGENPLHDEALDLIRRMRLPLRPPFPDEVIFNYGAIQFLQEVWRVLKPGGRA
                     ILVEFGIEDDWPSPVRLPGHTEYEVQFSHLRHVAKWLGFREQYCTLPQLLGMKRDINV
                     LCTGAAYTLRRFCRELEKPFSVRAYTEKELGTALGALLPKLTGLHYHNVLDPAWFGLW
                     DFKVLLVEKPGGMSIGQQPAFKESGGFRWYTQR"
BASE COUNT          882 a          885 c          980 g          888 t
ORIGIN      
        1 aaaatccgat ggtggttaaa tcgagccctt cttcccgcat ccagaatttg agggtagagc
       61 caatgagcag gaggggaaga ccggaagaaa agccggtcaa gagcatgacc agcattttgg
      121 gactggcaag aagaaggcag aaatttttga gccaatgatc cggctcggtc tgggtggtca
      181 tacaccagac cttatcaaag aaatgggctt aggtcttctt taggctgaag gtctgtgaga
      241 ggtgaggatg cggttcgtgg caggggttcg attagtttaa atttggatca accggcatat
      301 gcgcatacat cgtgtattca tctccgaagg atcgaaggac ctcgccccat aagtcctgtg
      361 aatcctcatc aaagacgagg tggggtctgg caggcatcgt gagccaggag ccactttcca
      421 tttcagactc caactgacct ggtccccagc ccgaatagcc catatatgcc cgaaacgatt
      481 cgtgttcacc gggaacttca agaatgcgct ccagcgtttc catattcccg ccgagataca
      541 ccccgtcaag aaccgcatgg gtatcttcaa tttcttcatt cagacggtac aggacgagca
      601 aactgttttt ttgaaccggt cctccggcat acacgcgatg ttcctggcct tcgagcactg
      661 gaacttgggg caggacttcg gcaatattca tttccgtcgg acgattgacg acaaccccga
      721 gggcgccatc gggtccatgc tcacaaagca gcacaaccgc ttggcggaaa ttgggatcac
      781 gcaatgctgg agtggcgatg aggaaaaccc ctttgcctaa tggtgtatcc atagtctaat
      841 cctaacgtgc cactgatgat ccatcaagag gcatttcttg ggaaaatggc ggcatggttg
      901 tccgtgaacc atcgtcaatc ctgtgaaatc caaccggtct tcaagcatca tgagactctg
      961 aactccgtcc tcagtgcctc acaaagccgg aggtagcact acgtgtgaaa agagtcctaa
     1021 tccaaaccct tcagaaattt gtagaaaata ttgatatatt aatgacggta atctttaaat
     1081 tcaccggtcg cgtcgtctaa tttcaaaaaa gttcgcatct gcgtcttaag ggtttgcctg
     1141 gcattttcat cccccaagtt caactggtac tcattaatga ccatcgtctt cgtcccacct
     1201 ggaccggccc attcattcca gcaggtttga cagactttct gcttaatttc ttcttccagt
     1261 tttcccatga aaagattgtc cgtgatcggg tccgcgtctt tatcgcattt tcggcaatga
     1321 atttgcgcca tccttttctc cttttgtaag tgataggctg aaagcctatc agtattgata
     1381 ggcaaaacaa tcaggaatcc taacacagaa atctcgggtg ggaaaagaag ccagaaagga
     1441 tttcaatgcc ctggtttttt tcgtgtcttt caggtatgga agtcgatccc acgtttcagt
     1501 ctcgttcaag tcaggacgag gattaccttg aaggggaagt tctgagatat atgtcacgat
     1561 tggagaagag acaacatgtt actcgcagga attgatattg gcacattaac ctgccgcttg
     1621 ttggtggcag aagtgaatcc accaggtgag tttacggttg ttgatgcgga tcggcgaatc
     1681 ttacgactcg gggagggagt ggaccaacac aaacggttat cacaagccgc gatggatcgc
     1741 gttgtttcca cacttataga ctggaaagaa aagaccacca agtatccgct cgacggagtc
     1801 gtagtcgtgg cgactagcgc cgtgcgcgaa tcagagaatc gtaaggactt tctcgcccgt
     1861 gtcactcaag aaaccgggtt ggaagtggag atcttaacgg gagacgagga agcccggcga
     1921 accttgctgg gtattcgttt tggcctgcct cccgccattg cgaatttttt tggattggat
     1981 attggcggcg gcagtaccga atgtattctg gctcattccg gaatggctcc ggccgtcatt
     2041 tcgttggatc tgggagtggt gcgcttatta gagcgggtgc ttcgtctgga tccacccaca
     2101 atccaggaga ttcaccaagc agaagcctgt attgatgagg aattggccaa ggtgtcgaaa
     2161 gctttcggga ccttttcagg catcccgctg gtcgggacgg cagggtccgt caccacactt
     2221 gccgcgatgt ctcaaggatt gccgagatat gagtcggcac gtgtccataa ttatgagtta
     2281 acgttgtcga gcatcaaagg attggagcag gatctcacca ccaaaaccgg tccgcaacgt
     2341 ttggccatgc cagggcttga acccgggcgg gaatatgtca tcgtggcagg caccgtgatt
     2401 ctccgaagag tcatggaaac cttcggcttt gacagatgtc tggtcagcga ttttggtctt
     2461 cgggaaggga tattggttga taaagccgga aaattgaaaa acttgggatg agaagggtgg
     2521 ggatgaatcc tatcgctggg tgtaccagcg gaagccgccg gattctttga aggccggttg
     2581 ttgtccgatg ctcataccgc cgggcttttc gaccaacagg actttgaaat cccagagacc
     2641 gaaccaggcc gggtccaaga cgttatggta atgcaggccg gtgagtttgg ggagaagcgc
     2701 accaagcgcc gtgcccagtt ctttttcggt gtaggcccga acggagaaag gcttctccaa
     2761 ttcacggcaa aatcgcctca gggtataggc tgcgccggta cataagacgt tgatgtcccg
     2821 tttcatgccg agcaattgtg gcaatgtgca atattgttcc cggaaaccca gccatttcgc
     2881 cacatgacga agatgactga attgcacttc atattcagta tgaccgggca atctgacggg
     2941 agacggccaa tcatcttcaa tcccgaattc gaccagaatg gcccgtccgc ccggcttcag
     3001 cacgcgccag acttcctgta aaaattgaat ggcgccataa ttgaaaatga cttcgtcggg
     3061 aaagggggga cggagcggca gacgcatgcg ccggataagg tccagggctt catcatgtag
     3121 gggattctcg cctttaaacg tgaggagttc gtccccggta aattggattg gcgtcatgtc
     3181 ggcgaggttt tcattgtcga tcaacaaatc aacggaacga tccgccaacg gcattcgttc
     3241 ggcattggct tgaatggccg tcgccgtcca ccctgcctgt cgcgccagcg atgtttggga
     3301 acccaggaac gggccggtca gatcgagaaa ggtgtactgg atgtctttcc tctcttcagc
     3361 cgagagttcc ctggctagtt ctttcgagac atacccgagg ccggcgccca cttccacgat
     3421 gcgtttgggc ttgggagaga gccagcccat ttgccgaagg gttttgccga gtagccgacc
     3481 ataggtaagc ccgttcatgg cttcgcaggg ttcacgaaat aaatgcgaaa cggtggtttc
     3541 gattatttca aaatgcccat ggggtccttg aataccctga acgtgaaatt tggacagatg
     3601 atcttcggtt tcagcctgga cgttttgatg ccacc
//
LOCUS       DAORAQ010000254         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3590_length_3631_cov_77.286074, whole genome shotgun sequence.
ACCESSION   DAORAQ010000254 DAORAQ010000000
VERSION     DAORAQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_3631_cov_77.286074"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PKM72_13730"
     CDS             complement(<1..1226)
                     /locus_tag="PKM72_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNP61901.1"
                     /translation="MMKRIVGLESEYGLTFSPNGRVYLPIEKILGYIFEGLIPNSWPS
                     NAFLTNGARFYQDTGCHPEYSTPECDDLLDLIIHDKAGERILESCLPIAEERLREEGL
                     SGEIFIFKNNTDSLGNTYGCHENFLMRRDVDFWKVSEQLIPFFVTRQIFSGAGKILKV
                     SGKSQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIILGDSNMSEF
                     STYLKVGTAMIVLSMIEDGFTIPHIELEEPVKAIRDISRDPTLKKTVKLEDGSSLTAL
                     EIQQTFWERAGEYLALQPPNKIMTEVHYEWGRVLKALNTSPMSLVREIDWITKQWLLE
                     NYMDKKSCGWDDPRLGMMDLQYHDINRQRSLFHLLAERNHIRKMIDEDAIEQAKTIPP
                     QTTRAKVRGDFIRFARA"
     gene            complement(1510..2193)
                     /gene="prcA"
                     /locus_tag="PKM72_13735"
     CDS             complement(1510..2193)
                     /gene="prcA"
                     /locus_tag="PKM72_13735"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNP61902.1"
                     /translation="MALPYYVSPEQMMQDRAEYAKKGIAKGRSIIAMEYSDGVLFVAD
                     NPSASLFKISEIYDSIAFSGAGRYSEFENLRKAGIQHADVKGMMYSREDVTARSLANG
                     YSQILGTLFSQEMKPYEVELLLAQVGETPERNELYRISFDGSIVDERRFTAIGGRSET
                     ILTLLRKEIPETCPSLKEALTLCQQAFEQGADSRENLEGLEVALLDRTRIGRRFRRVP
                     IIEASQILS"
     gene            complement(2197..3039)
                     /gene="prcB"
                     /locus_tag="PKM72_13740"
     CDS             complement(2197..3039)
                     /gene="prcB"
                     /locus_tag="PKM72_13740"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HNP61903.1"
                     /translation="MNFKDIPFPNFSADPCSSFYQYLTSQRPELLPMERWKTSLSQQG
                     QTDPSLMSSNQFPWPHGTTVLAFTYDNGVLIAGDRRATEGYQIAANRMEKVFQTDGYS
                     AMAIAGAAGPCVEMAKLFRIELEHYEKLDGQPLTCEGKANRLGHMVKANFPMVLHGLV
                     VIPLFVGYDHKREVGRLFKYDITGGRYEDTDFHAVGSGGKDARSTLKEYFRKNLPEDD
                     AVKVALRALINAADEDVGTGGPDLVRRIFPTVKLVDSQGVRDVEDTQLAALCEAIVNQ
                     KQET"
     gene            complement(3081..>3631)
                     /locus_tag="PKM72_13745"
     CDS             complement(3081..>3631)
                     /locus_tag="PKM72_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNP61904.1"
                     /translation="LQSAHDFYRTREVSPITKEVLVRWESTLATLERDPFELRREVDW
                     VAKHALIQSYMDRKQCSWKDPRVAMMDLQYHDVRPTKGLYYTLERTGQIERLTLDQEV
                     ERARQTAPAFTRAYFRGQCLKHLPKQVYGMSWTSVLLHSGNSTIKRIPLMDPYRGTQQ
                     LTQELFRDITSVDQLISRLGKS"
BASE COUNT          854 a          873 c          922 g          982 t
ORIGIN      
        1 gcacgggcaa atctgatgaa atctcctcgc acttttgcgc gtgtggtttg aggaggaatc
       61 gtttttgctt gttcaatcgc gtcctcatcg atcatttttc gaatatgatt acgttcagct
      121 aaaagatgaa acaggcttcg ttgtcgatta atgtcatgat attgcaaatc catcatgcct
      181 aatcgggggt cgtcccatcc acaagacttt ttgtccatgt aattttcgag aagccattgt
      241 ttggtgatcc agtcgatttc gcggacgagt gacatggggg acgtattcaa cgccttcaac
      301 actcgacccc attcataatg cacctctgtc atgattttat tgggaggttg caaggctaaa
      361 tattcacctg cccgttccca gaaggtctgt tgaatctcca atgcggtcag gctgctccca
      421 tcctcaagct tgactgtttt ttttagcgtg ggatcccgag agatatctcg aatcgcttta
      481 acaggttctt ctaactctat atgaggaatg gtgaaaccgt cttcgatcat ggagaggacg
      541 atcatggctg tccccacctt taagtaagtg gaaaactcag acatattgga atcgccgagg
      601 atgatatgca gtcgacggta tttttcggca tcagcatgag gctcgtcgcg ggtgttgata
      661 atgcttcggg aagacgtcgt ggaggaagac gttttttcat ggatatgttg cgcgcgttgg
      721 gagatgaagt attgagactt ccctgagact ttcaagattt ttccggcccc agaaaaaatt
      781 tgacgagtga cgaaaaaggg gatgagttgt tcgctgactt tccagaaatc cacatcccgg
      841 cgcatgagga aattttcatg gcacccatag gtgttgccga gggaatccgt attgttttta
      901 aagataaaga tctcgccgga tagtccctct tcgcgcaagc gctcttcggc aatgggcaga
      961 caactttcca ggatccgctc accggcttta tcgtggatga tcagatcaag cagatcgtcg
     1021 cattcgggag tggagtattc tggatggcag ccggtgtcct ggtaaaacct cgctccattc
     1081 gtcaggaagg cattggaagg ccagctattg gggatgagtc cttcaaaaat gtacccgagg
     1141 attttttcaa taggaaggta gacccgacca ttcggggaaa atgtcagacc atattcactc
     1201 tcaaggccaa caatgcgttt catcatactc ggcccgtccc tctccgcaga cactctaagg
     1261 acagatcgtg ccctgtcctc caccggcctc cttctcgttt aaaggaccta cccgccaatc
     1321 aatagaaatc ctcctcgagt ccttcggtct tgctgaatag gccttctcaa gagattcgat
     1381 ctttcacttt ccgtgttttg gaaatgaatc atggttgaaa acatggggat gactacactg
     1441 attcccatac tcagaataca cccagatcca tcaatgatca actgaatgag gggagcaaaa
     1501 caggagatgt tacgacagaa tttgagaggc ctcgatgatc gggacccggc gaaatcttct
     1561 acctattctg gtgcggtcca ggagagcgac ttccaagcct tctaaatttt ctcgagaatc
     1621 agccccctgt tcaaaggcct gttggcagag cgttaaggct tcttttaggg acgggcaggt
     1681 ttccggaatt tccttcctga ggagggtcaa gattgtttcg gaccgtcccc caatggcggt
     1741 gaatcttcgt tcatccacaa tactcccatc aaaggagatg cggtataact cattgcgctc
     1801 gggtgtctcg ccgacctggg ccaggaggag ttccacttcg tacggtttca tttcctggct
     1861 aaagagggtg ccgagaattt gtgaatagcc atttgccagg gatcgtgccg tcacatcttc
     1921 gcgactgtac atcatgcctt tgacgtcggc atgttgaatg ccggctttcc ggagattctc
     1981 aaattcgctg tatctccccg cccctgaaaa tgcgatgctg tcatagatct cagaaatttt
     2041 aaacagagaa gcactggggt tatcggcgac aaacagcact ccatccgaat attccatggc
     2101 tatgatggat cgacctttgg caataccttt cttggcatac tccgcccggt cctgcatcat
     2161 ttgttctggt gaaacgtaat agggcagggc cactgtttaa gtctcctgtt tttggttcac
     2221 tattgcttcg cacaatgccg ctaattgggt gtcttccaca tcccgaactc cctgagagtc
     2281 aactaatttt acggtcggga aaattcgtcg gaccaaatca ggtcccccgg ttccgacatc
     2341 ctcatctgcg gcattgatta aggcacgaag ggccactttg accgcgtcgt cttctggtaa
     2401 gtttttccga aaatattcct ttaaagtgga tcgggcatct tttccgcctg agccgactgc
     2461 gtgaaaatcc gtgtcttcgt agcgtcctcc ggtaatgtca tatttgaaga gccgcccaac
     2521 ctctcgtttg tggtcatagc cgacaaatag gggaatgacc acgagtccat gcaagaccat
     2581 agggaaattt gctttaacca tatgcccaag cctgttggct ttcccttcgc aggttaaggg
     2641 ctgtccgtca agtttctcgt aatgttccag ctcgattcgg aacagcttgg ccatttccac
     2701 gcagggccct gcagcccctg caatagccat ggcggaatag ccatcggttt gaaagacctt
     2761 ttccatgcga ttcgcggcga tttgatatcc ttccgttgcg cgacggtctc cggcgatgag
     2821 gacgccgtta tcataggtga aggcgagaac cgtggtgccg tggggccagg gaaactgatt
     2881 ggaggacatc aaggatgggt ctgtttggcc ttgttgggag agtgaggttt tccatcgctc
     2941 catggggagc aattcgggtc gttgggaggt gagatattgg taaaaactgg aacacggatc
     3001 ggctgaaaag tttggaaagg ggatgtcttt aaaattcatg tgacctcaat ggtaccttgc
     3061 cgaatgggtt ctctgttcat ttaggacttc ccgagtcggg atattaactg atccaccgag
     3121 gtaatgtccc taaacaattc ttgcgtgagc tgttgagtgc ctcgataagg atccatcaat
     3181 ggaatacgct taatagtgga atttcccgaa tgaaggagga cggacgtcca actcatgccg
     3241 tacacctgtt ttggcaggtg ttttaagcat tgacctcgaa aataagcccg agtgaaagcc
     3301 ggtgcagtct ggcgagcccg ttcgacttcc tgatcaagag tcagacgttc tatttggcct
     3361 gttcgctcca aggtgtagta cagccctttg gttggtcgca cgtcgtgata ctgtaaatcc
     3421 atcatggcca ctctgggatc tttccaggag cattgtttgc gatccatgta ggattgaatt
     3481 aatgcgtgtt tggcgaccca atcgacttcg cgccgaagct caaagggatc acgctccaag
     3541 gtagccaggg tggattccca gcgtacaagc acctctttgg tgatagggga gacctctctg
     3601 gttcgataaa agtcatgggc tgattgcaga a
//
LOCUS       DAORAQ010000255         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3611_length_3609_cov_153.949634, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000255 DAORAQ010000000
VERSION     DAORAQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3611_length_3609_cov_153.949634"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKM72_13750"
     CDS             complement(<1..224)
                     /locus_tag="PKM72_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNP61905.1"
                     /translation="MTLFQHILVPVDGSKHAAEALKFGLELAKLTEAQVHVLHVIDTA
                     SVSQISRLFGKSQAHIQEELRERAMGILVDA"
     gene            complement(229..846)
                     /locus_tag="PKM72_13755"
     CDS             complement(229..846)
                     /locus_tag="PKM72_13755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNP61906.1"
                     /translation="MRLELAATKANLLAVRETLALAREGHELLSQKREVLLTELLHLV
                     NDVKRVGKEANAALEEAYTSLKLVLTQTGHDGAQRLAQGCRERAEVTVRERSVMGVSV
                     PFVQVKTIPKRPMWGLGESPAVLDEMVERFQRALVLLGERAEIETAVWRLAQELAKTQ
                     RRTNALEYLVIPQHQETLKFIQETLEERERESHFQLKRIKALGRI"
     gene            complement(843..>1769)
                     /locus_tag="PKM72_13760"
     CDS             complement(843..>1769)
                     /locus_tag="PKM72_13760"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNP61907.1"
                     /translation="PVFSGSGLPHNELAAQIIRQAKLPTDTEAFAIVFVAMGIKHDEA
                     ELFRDTFETGNGLQQVVMFLNLADEPSMVRIIAPRVALTVAEYLAFDLGMHVLVILTD
                     MTAYCEAVREIASAKGEMPSRKGYPGYLYSDLASLYERAGRITGLPGSITQLPILTMP
                     NDDITHPIPDLTGYITEGQIVLDRHLLRKGILPPINVLPSLSRLMKDGIGPNRTRSDH
                     ANLASQLYAAYSRVRQLENLAAIIGEEELSAVDKQYITFGTRFEHQVIAQGVNEDRSI
                     VETLDHGWGALAELPPEELTRVTEDELKEHLT"
     assembly_gap    1770..1869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1978..>3609)
                     /locus_tag="PKM72_13765"
     CDS             complement(1978..>3609)
                     /locus_tag="PKM72_13765"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNP61908.1"
                     /translation="KGEEAVIQVYEDTTGLMAGVPVYGTGRPLSVELGPGLVGTIYDG
                     IQRPLARLIEKSGPWIDRGGQLAALDRNRAWNFQPRVSNGKSVAEGEVLGSVQETPLI
                     EHRILVPPGIHGNLVWLAPEGAYRVEEPVARIRADSGEIPITMLSRWPIRQARPGRRR
                     LLPTIPLITGQRVIDALFPLPKGGAAMIPGGFGTGKTVTQHNLAKWCEADLIVYIGCG
                     ERGNEMTGVLTEFPTLEDPKTGRPLIERTIMVGNTSNMPVAAREASIYTGVTIAEYFR
                     DMGLHVAVMADSTSRWAEALREISGRLEEMPAEEGFPAYLATRLAAFYERAGRVETLG
                     GVEGSMTIIGAVSPPGGDFSEPVTQQTRRFVRCFWALDRELASARHFPAIHPVDSYSE
                     YAQDLMAWWTEREPDWALLRSEALDLMQKDRRLQQMVKLVGEDSLPDDQKLALFGARL
                     WREAFLQQNAFDPIDRYTTPEKQVAMMKVLQHWIARATDVMKIHIPFYRVRELPVTEK
                     IARMKAIPNEELDTIGQIVTQIDAEMNQLEQTMQE"
BASE COUNT          766 a         1007 c          929 g          807 t
ORIGIN      
        1 gcatcgacga gaatccccat ggctcgctcc cgcaattctt cttggatgtg ggcttggctc
       61 ttgccgaaaa ggcggctaat ttgggaaaca gaggctgtgt caatcacgtg aaggacatga
      121 acttgggctt ccgtcagttt ggcaagttct agcccaaact tgagcgcttc cgcggcatgc
      181 ttcgatccat cgaccggcac cagaatgtgt tgaaacagtg tcattgaatc aaatcctccc
      241 cagggctttg atacgtttta actggaaatg tgactcccgc tcccgttcct ccaacgtttc
      301 ctgaataaat ttcagggttt cctggtgttg gggtatcacc aaatattcca aggcattggt
      361 tcgacgttgc gttttggcca actcttgggc taaacgccag actgccgttt caatttctgc
      421 gcgctctccc agaagaacca gggcgcgctg aaaccgttcc accatctcat ccaacaccgc
      481 cggggattct cctaatcccc acatcggtcg tttaggaatc gtttttactt gcacgaaggg
      541 cacggagacg cccatcacac tccgctcccg cacggttacc tcggcacgtt cccggcatcc
      601 ctgagccaat cgttgggcac catcatgccc agtctgagtc aaaaccagtt tcaatgatgt
      661 ataggcttcc tcgagagccg cgttggcctc cttccccact cgcttcacat cgttgacgag
      721 atggagcaac tctgtcagca atacttcccg tttttgggaa agcaattcgt ggccctcccg
      781 ggccaaggcc agggtttccc ggaccgctaa aagattggct ttcgtcgcag cgagctccag
      841 cctcacgtca gatgctcctt cagttcgtct tcagtgaccc ttgtcagttc ctcaggcgga
      901 agctcggcaa gagcgcccca tccatggtcc agggtctcca ctatcgaacg gtcttcattg
      961 accccttgtg caatcacctg gtgttcaaag cgggtgccaa aggtaatgta ttgcttgtcc
     1021 acagcggaga gttcttcttc cccgatgatg gcagcgaggt tctctagctg gcggacccgg
     1081 gaatatgcgg cgtacagttg gctggctaga ttcgcatgat cggaacgggt gcgatttggc
     1141 ccaatgccat ccttcataag acgggacagc gagggtagaa cattaatggg gggcagaatg
     1201 ccctttcgga ggaggtgccg gtccaagaca atctgtcctt ccgtgatgta ccccgtgagg
     1261 tctggaatgg gatgagtaat gtcatcgttg ggcatggtga ggatgggcag ctgggtgatt
     1321 gatcctggca atccagtgat acgcccggca cgctcataca aggatgccag atcgctatac
     1381 agatatcctg gatacccttt gcggcttggc atctcacctt tggcggaggc aatttcccgc
     1441 acagcttcac aataggccgt catatcggtc aggataacta agacatgcat gccgagatca
     1501 aaggccagat actcggcaac ggtgagggca acacgcggcg caatgattct gaccatcgaa
     1561 ggctcatcgg caaggttcaa aaacatgacg acctgttgca agccatttcc cgtttcaaag
     1621 gtatccctga aaagttcggc ttcatcatgt ttgattccca tcgccacgaa gacaatagca
     1681 aaggcttcag tgtccgttgg caatttggct tggcgaatga tttgcgcggc cagctcattg
     1741 tggggaagcc cactccccga gaagaccggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnt cgaaccatct cgccataccc gacacccgca ataccgttga tgacgagtaa
     1921 ggggccgttc agttcgctga ggccgtggca ttcttttccg gaaatccgcc gcacgtttca
     1981 ctcctgcatt gtttgttcga gctgattcat ctcggcatcg atctgcgtca caatctgacc
     2041 aatagtatcg agttcttcat tgggtatcgc tttcatccgc gctatttttt ctgtgacagg
     2101 aagttcccgc acacgataaa acgggatgtg gattttcatg acgtcggttg cccgagcaat
     2161 ccaatgctgc aaaactttca tcatcgcaac ctgcttctcc ggggtagtgt accggtcaat
     2221 gggatcaaag gcattctgct ggagaaaggc ttcgcgccac agccgtgccc caaagagtgc
     2281 caacttttgg tcatccggca gcgagtcttc gcccaccagt ttgaccatct gctgaaggcg
     2341 acgatccttt tgcataagat ccaaggcttc tgaacggagt aacgcccagt ccggttctcg
     2401 ctcagtccac cacgccatga gatcttgtgc atattcagag taactgtcga cagggtgaat
     2461 agccggaaaa tgacgtgcac tcgccagttc acggtcaagg gcccagaaac accggacaaa
     2521 tcgacgagtt tgttgagtga ccggctcgga aaaatcaccg ccaggcggtg agactgcccc
     2581 aataatcgtc atggagcctt caactccgcc aagggtctcc acacgcccgg cacgttcgta
     2641 gaatgcggct aaccgggtgg ccaggtaggc aggaaagccc tcttcggccg gcatttcttc
     2701 caatcgtccg gaaatttccc gcaaggcttc tgcccaacgc gaggttgaat cggccatgac
     2761 ggccacatgg aggcccatat cccgaaagta ctcggctatc gtgacgccgg tatagatact
     2821 cgcttcccgt gcggcaaccg gcatgttcga agtattgccc accatgatcg tccgttcgat
     2881 gagggggcga ccggttttgg gatcttccaa tgtcggaaac tccgtcagaa ccccggtcat
     2941 ttcattccca cgctccccac atccaatgta gacaatgagg tccgcctcac accatttggc
     3001 caggttgtgc tgggtgacgg tcttgcccgt gccgaaaccg cctgggatca tggcggctcc
     3061 tcctttgggg aggggaaaca aggcatcgat gacacgctgg ccggtgatca gggggatggt
     3121 gggaaggagt cgacgacgtc ccggccgtgc ctggcgaatc ggccatcggg aaagcatggt
     3181 gatcgggatt tccccggaat cagcgcgaat gcgcgcaacc ggttcttcca cgcgataggc
     3241 tccttccggt gcgagccaga cgaggttacc gtggattcct ggaggaacca agattcgatg
     3301 ttcgatgagt ggagtctcct gcacacttcc cagtacctcc ccctcggcca cggatttgcc
     3361 attggaaacc cgtggttgaa aattccatgc ccggttccga tccaatgcgg ccaattgccc
     3421 cccacggtca atccaggggc cactcttttc aatcagacgg gcaaggggtc gttggatgcc
     3481 gtcataaatg gtgcccacta aaccagggcc aagttcgacc gaaaggggcc tcccggtgcc
     3541 atagactgga actccagcca tgagcccagt cgtatcctca tagacttgaa tcacggcctc
     3601 ctctccctt
//
LOCUS       DAORAQ010000256         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3663_length_3565_cov_72.990883, whole genome shotgun sequence.
ACCESSION   DAORAQ010000256 DAORAQ010000000
VERSION     DAORAQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3663_length_3565_cov_72.990883"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKM72_13770"
     CDS             complement(<1..1007)
                     /locus_tag="PKM72_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNP61909.1"
                     /translation="MSSQPGRHLVDGTVRVFLAGLLFPLTGLITAGFLTRRLGTDGYG
                     LLVLSATLFGWVELGINSFFARPAIKFIGEAKNWLPVGSTLIRLQFVAGVVGGVLLGL
                     LAFPLAWLLDEPLLAPYLALFALHIPISSLTQAHQSILVGIGNFRQKAVTNACRWIAR
                     LLLILVLVEMGLSVPGAIIGSLGASLVELAVSRRYVRPPLFGKVAILTRPFYDFGILL
                     CVSSILFLIFSSMGLVMLKILGGTLEAAGIYGAAQNLSIIPGLFGMSFSPLLLSTVSR
                     LLFEGRIDQAKSMGFGAMRMVVGLFPFGALVAGAAPEIVTLVFGQSFELAGPILGVLI
                     MG"
     gene            complement(1013..2011)
                     /locus_tag="PKM72_13775"
     CDS             complement(1013..2011)
                     /locus_tag="PKM72_13775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNP61910.1"
                     /translation="MPKRLKRILLYAGYLLLVTLPLLEIGVRIWGYSEHHIHDPIYTS
                     FESSEDIPYIHKPNLLQARARGLAVINTDSLGLRSKAAGAVYGLKQPQEYRIAIAGDS
                     VTFGEGIPNTDGTFPEVLEHILNQQQNIQTVKVFNFGVSAYSVREMAALLQHRMLAIQ
                     PDLVIMAIIPPDLDLKRTPIIDAAGYLVGQNVAMLLDSRVGEVLRGIRLLYVLRDIGS
                     RWISPPQYIDPLLSHGKIPDSYRYIQQFKETADQYGLPYLIVLLPRMEENAWGILSDR
                     LTQDTIKHLDLSYLRKEFTMNKYMASRFDRHPSPAVHRRIGEELALYVEHQPEFPQ"
     gene            complement(2915..3409)
                     /locus_tag="PKM72_13780"
     CDS             complement(2915..3409)
                     /locus_tag="PKM72_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61911.1"
                     /translation="MFISAKWVIQHFDLTLSRCMVAIEGFSKVGSALVTLLDEVNAKV
                     VAISTERGAITNPHGLNVRQLNQLAAQYGSHLMDFYTDAEGICCADLFELTVDLLCPC
                     ATHPSLHADDASRVPARLICPGANEPVTAEAEKILFELGLMDLPGFVTNCGGMLGSTM
                     ALRR"
BASE COUNT          990 a          964 c          821 g          790 t
ORIGIN      
        1 cccataatca acacccccag gattggacca gcaagctcga aagactggcc aaacaccaat
       61 gtgacaatct caggagcagc acccgcaacg agagctccaa aggggaataa acccacaacc
      121 attctcattg caccaaatcc catacttttt gcctgatcaa ttctcccctc aaaaagcaga
      181 cggcttactg tcgagagcaa taatggagaa aacgacatcc cgaacagacc aggaatgatc
      241 gacaaattct gagccgcacc ataaattcct gcggcctcca gtgttccgcc aagaattttc
      301 agcataacca agcccatgct agaaaaaata aggaacaata tggatgaaac acacaggaga
      361 ataccgaagt cataaaatgg gcgagtgaga atggcaacct tgccaaagag gggaggcctg
      421 acatatcgac ggctgacagc gagttcgaca agggacgccc caagacttcc aattatagcg
      481 ccaggaacag acaggcccat ttcaaccagg accagaatca acagcaacct ggcaatccaa
      541 cggcaagcat tggtcacggc tttttgccga aaattaccaa ttcccacgag aatactctgg
      601 tgcgcttgag tcaggctact gattggaata tggagggcaa agagggcgag atacggagcg
      661 agcagcggtt cgtcgagcag ccaggcaaga ggaaacgcca gcagtcctag taacacgcct
      721 ccgacaaccc ctgcaacgaa ttgcaaccgg atgagggtgc taccaactgg cagccagttt
      781 ttcgcttccc caataaactt aatcgctgga cgcgcaaaaa atgagttaat cccgagctcc
      841 acccatccaa acaacgttgc ggagagcacc aataagccat agccatccgt tccaagtcgt
      901 cgggtaagga accctgcggt aatgagtccg gtaagcggaa acaggagccc ggcgagaaag
      961 actcgtactg tcccatccac aaggtgacgg cctggttggg acgacaaggg actcactggg
     1021 ggaactctgg ttggtgttcc acatacagcg ctaattcttc cccgattcga cgatgaaccg
     1081 caggggaagg atgccggtca aaccggcttg ccatatactt gttcatagta aattctttac
     1141 gtaagtatga caaatcaaga tgcttgatcg tgtcttgtgt cagtctgtca gaaagaatac
     1201 cccaggcatt ttcctccatt ctgggaagga gcacgatcaa gtagggaagt ccgtattgat
     1261 ccgctgtctc cttgaattgc tgaatgtacc gataagagtc gggaatcttc ccatgtgaaa
     1321 gcagtgggtc aatgtattga ggtggggaaa tccagcgtga gccgatatcc cttaggacat
     1381 acaaaaggcg aattcctctc aaaacctctc cgacccgaga atccagaagc atagcgacat
     1441 tctgtccaac cagataccca gctgcatcaa taatgggggt ccgcttgaga tccaaatctg
     1501 gtggaatgat tgccatgata accagatccg gctgaatggc cagcatccgg tgttgaagca
     1561 gggcggccat ctctctcacg ctataggccg acaccccgaa attaaacact ttcacggtct
     1621 ggatattttg ttgctgatta agaatatgtt ccagaacttc tgggaatgtt ccatcagtat
     1681 tggggatgcc ctcaccaaac gtgacggaat ccccggcaat ggcaatgcga tattcctgtg
     1741 gttgtttgag gccatagacc gccccggcgg cttttgaacg caatcccagg ctatccgtgt
     1801 tgatgacagc aagccctcgg gctcgggctt gcagaagatt gggcttatgg atgtagggaa
     1861 tgtcctcgct cgattcaaat gacgtataga tcggatcatg aatatgatgc tccgaatacc
     1921 cccagatcct gaccccaatt tccaaaaggg gtaaggtcac gaggagcaga taacctgcat
     1981 acaatagaat ccttttcaat ctttttggca ttgatgattc tttcttaact aagccaaccg
     2041 tggcggcttc gactgccccg ttttcaattc gtaaaatgat tcagtatcga taaaacagga
     2101 ccttcctaga aggaatcaat cctaacacgg catcaaaatt ccggcattgg aattaaaatg
     2161 actcgacaag cataaatttc tctggtggca tcttttcttg gtccgcccat gccggcaggg
     2221 ctcgataaag gtttcccaca attcctcctc ccatgctgcg tagcccgaaa ccacaatgac
     2281 cgataggcca gcttctttgc tatttgttgc ctgtcattgc ttgatcatgg cgattaatag
     2341 ccaacccacc ccactggaat tgtcagaatc aaggaatgaa gcggagtctt gccctgatcc
     2401 atatttcctg catgaacttg ttgatcctcc actttgcctt ttttttcgta aaccatttca
     2461 agggatgcca cgcccattcg ctccgggccc gagtgatctc tttcactttt ctccacaagt
     2521 catatcaata atgggagtgt taaaaaattc tgcctggccg gacacgcaca aggaatcggt
     2581 aaatatcaca gaccaccgac gaagaaccat atacacctgg gacaccgtct cattcaccac
     2641 cattcatgcc catctctttg ctaattgcga ttgcgccagg accgccaaaa gagaccccgg
     2701 cacccaaccc cgagtataga gttctacccg agcggcaacg agcttattgc gtagagatgg
     2761 ggggcagcgc ttcgctgaat ctccccaaag cgttgtcgtg cgaactgttc ggcaatctcg
     2821 cgcgatgata cctgccggtg catcgcctcc cttactaggc acgcaacgcg atcgccgaaa
     2881 tgaagctcca taaccgacat gatctgctat ctgctcatcg acgcaaagcc atcgtactcc
     2941 cgagcatccc gccacagtta gtcacgaaac cgggaaggtc catgagtccc agttcaaaca
     3001 gaattttctc ggcttccgcc gttactggtt cattggctcc tggacagata agacgcgcgg
     3061 gcacacgaga agcatcgtca gcatgaagac tgggatgggt cgcacaagga cacagcagat
     3121 cgacggtcaa ctcaaacaag tcagcgcaac aaattccctc ggcatctgta taaaaatcca
     3181 tcaaatggct gccatactga gcagcgagtt ggttgagctg tctcacattc agaccatgcg
     3241 gatttgtaat cgctcccctc tctgtagaga ttgccaccac tttggcattc acttcgtcca
     3301 acagggtgac caacgcgctc cccaccttac tgaatccttc aatggctacc atacaacgag
     3361 aaagcgtcaa atcgaaatgc tggattaccc atttggcact gatgaacaca atcgccgccg
     3421 tataatgccc ctattgcgta tcgcgtaact tcgtcaactt aagcttcaca ccaaccgcat
     3481 tgagcatcgc gcggatgtct gtgtcatttg tacccatatc agagccaaga ataaagatgc
     3541 ggttaattag aagcggagca atcgc
//
LOCUS       DAORAQ010000257         3560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3669_length_3560_cov_62.876462, whole genome shotgun sequence.
ACCESSION   DAORAQ010000257 DAORAQ010000000
VERSION     DAORAQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3669_length_3560_cov_62.876462"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKM72_13785"
     CDS             complement(<1..788)
                     /locus_tag="PKM72_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61912.1"
                     /translation="MCGIAGELRFNQAVESKADWEKISALMARRGPNDQGIWTDHKTC
                     TLVFRRLAIIDLSMNAHQPMTAHNGRYTLVFNGEIYNFQDLRKKLEGRGVRFRSTSDT
                     EVVLYALMEWGTTALERFNGMFALGFFDSVEKRLLLARDHAGMKPLYFLKKAEGMVFA
                     SQYNQILAHPLSHNLEVSPEALGLYLRLAYIPAPYALMQDSQMLEPGTWVQFTGNGQI
                     EKGQYFKFPQYEMPKLKGQEALEAVDSAITGAVKRHLISDVPVGA"
     gene            complement(875..2053)
                     /locus_tag="PKM72_13790"
     CDS             complement(875..2053)
                     /locus_tag="PKM72_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNP61913.1"
                     /translation="MSSHQSVVFQYIKIPNENKRFFCLQQNILVLAWMLFFIKDSLTP
                     IVRPDSQISRVGLLIKQMHSYLGFVKRNVSCLSFFGGYKLQLLSRQGVVELPCYGQIC
                     VPIHKGYKVFDFHRGIAIKVFDHDVNASAIRDEIEKLKVVSKIEFASSIKKWSIEERW
                     YEEEYVNGALDSSYKPLDSVALLEKFFNEIIQYLTHLILFQKPISKNAVEHALDRNRE
                     LRSKLSSKPTFTGSEHQRITLFIDSIMNRLQTEGNCPIFLVFAHGDFVPANMLNTQHG
                     MKIIDWEGAGYWSALFDFYSYFFYRPACREVPVSMLGSEVMKALPMFISKLTEHAPEV
                     SSSLKAQEKTYRWIFYIEMIYRLVDRQMTDTNLDVMHYIQEYIKAFNLYEELAIGKNV
                     "
     gene            complement(2261..>3560)
                     /locus_tag="PKM72_13795"
     CDS             complement(2261..>3560)
                     /locus_tag="PKM72_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP61914.1"
                     /translation="CYRIGLKETIQDNLFNTVWNFIKNPLKNYRVYRSLYNFDDVNPD
                     QDTNPPDVIWAVKNVSFDVKEGEVIGIIGSNGAGKSTLLKILSKITYPTSGQVTIRGR
                     LSSLLEVGTGFHPELTGRENVYLNGTILGMRKKEIDRKFDEIVDFSGVEKFIDTPVKR
                     YSSGMKVRLAFAVAAHLEPEILIVDEVLAVGDARFQQKCLDKMKEVGQHGRTVLFVSH
                     NMPAVTRLCSRSILLDGGRVQQDGPTHEVITSYMNSDKCTKAERRWTNVMEAPGDEVV
                     RLCGVRVRTQDGLVSDVANISQPVRIEIEYEILQPGYVLRIYYHVFNEEGIEAFLPIE
                     NDMAWRQKPRPIGRYVSTSVIPENLLSEGRYFISPTIGTENPWVKRLRVNDVVAFHVI
                     DSMDGNGARADSVGHIPGVVRPLLKWETQFNPKEAYVEKT"
BASE COUNT         1006 a          861 c          667 g         1026 t
ORIGIN      
        1 gctcccaccg ggacatcact aattagatgc cgtttcacag cccctgtgat tgccgaatcc
       61 accgcctcca gagcctcttg cccttttaat tttggcattt cgtactgtgg aaatttaaag
      121 tactgcccct tctctatttg tccattcccc gtgaattgga cccaggttcc aggctcaagc
      181 atctgggaat cctgcatgag agcataaggc gcaggaatgt atgccaaccg caaatagagg
      241 ccaagtgctt ctggtgaaac ttcaagattg tggctcaagg gatgcgccaa tatttgatta
      301 tattgtgatg cgaataccat cccttcggcc ttctttagaa aatatagcgg tttcatccca
      361 gcatggtcgc gagccaatag caatcgcttt tctacagaat caaaaaaacc caaagcaaac
      421 atcccgttaa aacgttctaa cgcagtcgtt ccccattcca ttagggcata cagcacaact
      481 tcggtgtcac tagttgaccg gaatcgtact ccacggcctt ctaatttttt ccgcaagtcc
      541 tgaaaattat aaatctcccc attaaaaacg agtgtatagc gaccattatg agccgtcata
      601 ggttgatggg cattcatact taaatcaatg atggccaaac ggcggaagac caaggtacag
      661 gttttatggt cggtccaaat tccctggtca ttgggtcccc gacgtgccat caatgcacta
      721 atcttttccc aatcggcttt cgactccacc gcctgattga atcgcaattc tccagctatc
      781 ccgcacatgt tctatttttt tccctcaatt caacaaaact tcaagatgaa tatcggcgat
      841 gccgctaaaa tgtctggctt agatttctga aacgtcatac gtttttaccg atagccagtt
      901 cttcgtaaag attaaaagcc ttaatgtatt cttggatata atgcataaca tccagatttg
      961 tatctgtcat ttgccgatcc actaatctat agatcatctc aatataaaat atccatcgat
     1021 aggttttctc ctgcgccttt agactacttg aaacttcggg agcgtgttcg gttaattttg
     1081 aaataaacat cggcagggct ttcattactt ctgaacctaa catgctcacc ggcacttccc
     1141 tacaagcagg tcgataaaaa aaataactgt aaaaatcaaa taatgcactc caatatccag
     1201 caccctccca atcaataatt ttcattccat gttgcgtatt gagcatattg gctggaacaa
     1261 aatcaccatg ggcaaacacc aagaatattg ggcaatttcc ttccgtttgg agccgattca
     1321 taatggaatc aataaataat gtaattcttt gatgctctga tccagtgaac gtcggctttg
     1381 acgatagctt tgaccttaat tccctgttcc tatcaagagc atgttctaca gcatttttgg
     1441 atattggctt ttgaaacagt attaaatgtg tcaaatactg aataatctca ttaaaaaact
     1501 tctctaataa cgcgacggaa tctaaaggct tatatgagga atccaatgct ccattcacat
     1561 attcttcctc gtaccatcgt tcttcaatgc tccacttttt tatagaagag gcaaattcga
     1621 tcttggagac caccttcaat ttttcaattt catcacggat agcggaagca ttcacatcat
     1681 gatcaaacac cttaatagcg atcccccgat ggaaatcgaa tactttatac cccttatgaa
     1741 taggcacaca tatttgacca tagcatggca attcaacgac cccttgcctg cttaacaatt
     1801 gaagcttata acccccaaaa aagctaagac agctaacatt tcttttcaca aatcccagat
     1861 aactatgcat ctgctttatc aacaacccca cacgagaaat ttgggagtca gggcggacaa
     1921 taggggttaa ggaatccttt atgaaaaaca acatccaagc taaaacaaga atgttttgct
     1981 gtaaacaaaa aaaccgttta ttctcatttg ggatctttat atattgaaag actactgact
     2041 gatgagagct catctatgaa ttatccgaat cttttttatt tgggataggt aaaatcaagc
     2101 tggtgcttca gtcacaccac catgtgtgtg attcactttt gaaattttgt ggattacagt
     2161 attttctgga gtaagttgaa ggaaatgttg cgcagtgaga tattggagaa agagcgaatc
     2221 ctctggagaa aatagaaatg ttatattcct cagtttacac ttaggtcttt tctacgtatg
     2281 cttcttttgg gttaaattgg gtttcccact ttaggagcgg ccggactacg cccgggatat
     2341 gtccgacaga atcagcacga gccccattac catccatact gtcaatcaca tgaaatgcca
     2401 ccacatcatt aactctcaag cgcttcaccc agggattttc ggtgccaatc gtcggcgaaa
     2461 taaaatacct cccctcggaa agcaaatttt cagggatcac ggacgtactc acgtatcgtc
     2521 caatggggcg cggtttctgc ctccaggcca tatcattttc gatcggtaaa aaagcttcaa
     2581 taccctcttc attgaaaaca tggtaataga tccgcagcac atagcccggt tgaagtattt
     2641 cgtattctat ttcaattcga acaggttgac taatatttgc aacgtccgac accaggccat
     2701 cctgtgtccg cactcttact ccgcataatc gaacgacttc gtctccaggt gcctccataa
     2761 cattagtcca tctgcgctca gctttggtac atttatctga gttcatgtaa cttgttatga
     2821 cttcatgggt tggaccatcc tgttgcactc ttcccccatc aagcaaaatc gacctggaac
     2881 atagtcgagt gacggccggc atattgtgcg agacgaacag tacggtgcgc ccatgttggc
     2941 caacctcttt cattttatcc agacatttct gttggaaacg tgcgtcgccg accgccaaca
     3001 cctcatcaac aatcaaaatc tccggctcaa gatgagcggc gacggcaaat gccaggcgca
     3061 ctttcatgcc actcgaatac cgtttcacag gggtatcgat aaacttttct actcctgaaa
     3121 aatccacaat ttcatcaaat ttcctgtcga tttctttctt ccgcatgcct aaaatggtcc
     3181 catttaggta gacattttct ctccctgtca gctccggatg aaatccggtt ccaacctcga
     3241 gaagacttga aagccttcca cggatcgtga cctgcccact ggttggataa gtaattttcg
     3301 acagaatttt caggagagtg gatttgccag cgccattact gccgataatg ccgataactt
     3361 ccccttcttt cacgtcaaac gagacatttt tgacagccca gatgacatcg ggaggattgg
     3421 tatcttgatc gggattcaca tcgtcgaaat tatagaggga gcgatagacc cggtaattct
     3481 tcagaggatt tttgatgaaa ttccatacgg tgttaaataa attatcctgg atggtctctt
     3541 tcaatccgat ccggtagcat
//
LOCUS       DAORAQ010000258         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3681_length_3552_cov_69.190735, whole genome shotgun sequence.
ACCESSION   DAORAQ010000258 DAORAQ010000000
VERSION     DAORAQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_3552_cov_69.190735"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3314)
                     /locus_tag="PKM72_13800"
     CDS             complement(<1..3314)
                     /locus_tag="PKM72_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNP61915.1"
                     /translation="MKKNSPIPDPDFEHTSEVNPPNSATNLDVPDIQEGHPKTGVENR
                     LIQGALEHTREALIVCDASGTIIRTSPAVNHYCQHPCVNRPFDEAFHLSNFNGKKSPS
                     GASSPNLSRKLLSQVFSGVTLYREKVHLEKQDGRVEQIVFSARPIKDDRQHIIGGVVT
                     LMEIDSGKRTDQILRHQNEVLEQIINDTSLSDILDNLCLESERHLGSDVLAAILLLDE
                     DGCHLRHGAGPSVAESYKKAIDGVAIGPEVGACGAAAYLKEPVIIPDISTHPNWAPFK
                     DLAERHGLRACWSMPILASDGHTLGTFGMYYTVPRLPTDEETEYVELLAHSASIAIEK
                     VRAKEQLRQQTHALEVVNQIGRSLAAELNLEKLVQAVTDACRELTGAEFGAFFYNVMD
                     EKGESFVLYTISGVPREAFEKFPMPRNTPIFAPTFSGQAIVRIDDVTVDPRYGQLPPF
                     HGMPPGHLPVRSYLAVPVVSRTREVLGGLFFGHSQPKVFTHTAETMVNGIASGAAIAI
                     DNARLYQSAQNEIEQRKHTAENLKRLASIVESSQDAIYSINTDGIIMSWNNGAEKIYG
                     YTASEMFGSSFSCLLLPDARQDASSQLEFILKQNGNMTHEVLRMKKNGVVFPVSLSIS
                     PIVDVDGVLSGHSLIERDVSEQKRIGEALQSSESRLHAIWENSPTVKFVKDLEGKYQL
                     VNKRFESILQKSREMIIGKTDWDLLSPEFAGQLVAHDQQVLSSRKPQEFEEQIQSPHG
                     VCTYLATKFPLYNADGIPYAVCGIATDITAQKRAEQDLKELNETLERRVTERTRDLVG
                     YQANLRGMASELVVTEQRERRRLATELHDYLAQLLVVCRMKLTQARNDWSLPALKSNL
                     EEIDKILSDSLSYTRTLIAELSPTILYELGLVPALKWLGQQMERHGLHVQVQASDQSV
                     RLPEDHAIFVFQAVRELLFNVIKHSGVSNATVSLDGQSEQNLEVLVQDGGHGFQPTLE
                     STDYTNPGKFGLFSIRERAEALGGLLFIESAPGQGTRAKLVVPFQATSAPVSANSAFT
                     ESLKPPNESLPRPQKKKVVRILLVDDHAMIRQGIRTLLEHHDDFMIVGEAKNGEEALK
                     VTNLVLPDVVVMDVNMP"
BASE COUNT          923 a          910 c          820 g          899 t
ORIGIN      
        1 ggcatgttga catccatgac caccacatca ggaagaacca ggttcgtcac tttcagagct
       61 tcttctccat ttttcgcttc accaacgatc ataaaatcat catggtgttc caataacgtc
      121 cgaattcctt gacgaatcat ggcatgatca tcaaccagaa gtattcgaac gactttcttt
      181 ttctgaggtc gtggcaacga ttcattcggc ggcttgaggg attccgtaaa tgcggaattc
      241 gccgataccg gtgcagaagt cgcttggaag ggaacgacga gtttggcccg agtcccctgc
      301 cctggggcgg attcgatgaa aaggagacca cccaacgcct ccgctcgttc tcgaatacta
      361 aacaagccga attttcccgg attcgtataa tccgtggatt ccaaggtcgg ttgaaatcca
      421 tggcccccat cctgaaccag gacttcaagg ttctgctcgg actgaccatc aagagaaaca
      481 gtcgcattgc tgacaccgga atgcttgata acattgaaga gcaattcgcg aacagcctga
      541 aacacaaaaa tggcatgatc ttcaggaagg cggacggact gatcacttgc ctgcacctgc
      601 acatgtaggc catgtcgttc catttgctgc cccaaccatt tcagggccgg caccagaccc
      661 aattcgtaca aaatggtggg actgagctcg gcaatcaacg tgcgggtgta ggaaagcgag
      721 tcagacagga ttttatctat ttcttccaga ttcgacttta atgcagggag actccaatcg
      781 ttcctggcct gagtcagttt catccgacac accacaagta attgcgccag atagtcatgc
      841 agttcagtgg ccaatcgacg gcgttcccgt tgttccgtaa caaccaactc agaggccata
      901 cctcgcagat ttgcctgata tcccaccaga tcgcgagtcc gttccgtcac ccgccgctcc
      961 agggtttcgt tcaactcttt caggtcctgc tctgcccgtt tttgcgccgt aatatcggtc
     1021 gcgattccac aaacggcata gggaatgcca tcggcattgt ataaggggaa tttcgttgcc
     1081 agataggtac atactccatg cggtgattgt atctgttctt caaattcctg aggttttcga
     1141 ctgctgagaa cctgctgatc atgcgcgact aattgaccgg caaactccgg actcaagagg
     1201 tcccaatctg tttttccaat aatcatttca cgcgattttt ggagaattga ttcaaaccgt
     1261 ttattaacca gttgatattt tccttctaaa tccttcacaa atttcacagt aggcgaattc
     1321 tcccaaatcg catggagacg actttcactc gattgaaggg cttctccaat gcgtttttgt
     1381 tcggaaacat cccgttcaat caaggagtgc ccactcagga ctccatccac gtctacgatt
     1441 ggagaaatcg ataacgacac aggaaacacc acaccgttct ttttcatccg gagcacttca
     1501 tgagtcatgt taccgttttg tttcagaatg aattccaact gggaagaagc gtcctggcga
     1561 gcatccggga gaaggagaca ggaaaaagac gaaccaaaca tttcagaagc ggtatagcca
     1621 tatatttttt cggcaccatt attccaactc ataatgatac cgtccgtatt gatactataa
     1681 atggcatcct gagatgattc gacaattgac gccaaccgtt taaggttctc tgctgtgtgt
     1741 tttcgctgtt ctatttcatt ctgggcggat tggtagaggc gggcattatc gatggcaatg
     1801 gcagctcccg atgcgatgcc gttcaccatc gtctccgcag tgtgggtaaa gactttgggc
     1861 tgtgaatgac cgaaaaataa tcctcctaac acttcccggg tgcgggaaac gaccggaacc
     1921 gcaaggtagc ttctgacggg aagatggccg ggcggcatgc catggaaggg aggcaactgt
     1981 ccatatcggg gatcgaccgt cacgtcgtca attcgaacga tggcctgacc cgaaaaggtc
     2041 ggagcaaaaa ttggtgtgtt tcttggcatc gggaattttt caaatgcctc acgaggaact
     2101 ccggaaatcg tatataaaac aaaagattcc cctttttcat ccatgacatt gtaaaaaaat
     2161 gccccgaact ccgccccggt taattcacga cacgcatccg tgaccgcttg aacgagtttt
     2221 tctaagttca attccgctgc caacgacctg ccaatttgat tcaccacctc caacgcatga
     2281 gtctgctgcc gtagctgttc cttcgcccgc actttttcaa tggcaatgga ggcagaatga
     2341 gcaagaagct ccacatactc tgtttcttca tccgttggta aacgggggac cgtataatac
     2401 atgccaaagg taccaagcgt atgaccgtcg ctggctagaa tcggcataga ccagcatgcc
     2461 cgtaacccat gtctttctgc gagatccttg aatggagccc aattcggatg ggtggaaata
     2521 tccggaataa tgacgggttc cttcagatac gcagccgcac cacacgcacc cacctcagga
     2581 cctatggcaa cgccatcaat agctttcttg taactttcag ccacggaggg gcctgcccca
     2641 tgtcgaagat gacagccatc ctcatccagc aaaagaatag ctgccagcac atctgaaccc
     2701 aaatggcgct cactttccag acagagatta tccaaaatgt ccgaaagaga ggtatcgttg
     2761 ataatttgtt ccagcacctc attttgatgc cgaaggattt ggtcggtccg tttcccggag
     2821 tcgatctcca tcaacgtcac tactccacca ataatatgtt gacggtcatc ttttataggc
     2881 ctggcactga atacgatctg ctcaaccctt ccatcctgtt tttccagatg aactttttcg
     2941 cggtaaaggg tcacgccgct gaagacctgt gaaaggagtt ttcgactcag atttggagaa
     3001 gaagcgcccg aaggagactt cttcccatta aagttagata aatgaaaagc ttcatcaaaa
     3061 ggccgattca cacacggatg ttgacagtaa tgattgactg ccgggctggt tcgaatgatg
     3121 gttcctgatg catcacagac gatcagggct tctctcgtgt gctctaaggc accctgaata
     3181 agtcgattct cgaccccggt cttaggatga ccttcttgta tgtccggcac atcaagattt
     3241 gtagccgaat tgggaggatt gacctccgat gtatgttcaa aatctgggtc ggggattggc
     3301 gaatttttct tcatggaaca cattccgggt acggcctctt ggaagtcatt cgtatggagg
     3361 cctaggtgtg gtatttatac aataggttct ggccactatc ctcaactttc ctgagaaagt
     3421 caaactgtca aaaacccttg attgccatcc taaaatcccc cgggaggaac agggcgacca
     3481 ttacacgcat agagggaacc agagcttgga atttaaaaag aaccgccaca gatggtgtac
     3541 atttcaagga gc
//
LOCUS       DAORAQ010000259         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3722_length_3521_cov_68.769186, whole genome shotgun sequence.
ACCESSION   DAORAQ010000259 DAORAQ010000000
VERSION     DAORAQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3722_length_3521_cov_68.769186"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..842
                     /locus_tag="PKM72_13805"
     CDS             273..842
                     /locus_tag="PKM72_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNP61916.1"
                     /translation="MRKASRNARSFRCSLRSGRRRFLQRVISRAVVGVAAQAGINFVS
                     PQDLRAQTNLNPDGALQELLAGNRRFAADQLTSIEPDLAILKAHTVDKQEPFAAILAC
                     ADSRIPVELVFDQTIGHIFVTRVAGNVVTPQIIASLEYGVAVLGVKVLVVVGHSGCGA
                     VRAAMKPDTVPEQISVLYQHLLPNGTVWR"
     gene            1590..2444
                     /locus_tag="PKM72_13810"
     CDS             1590..2444
                     /locus_tag="PKM72_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNP61917.1"
                     /translation="MTRTWFWGIVGILFIINGLSQPAWSQESKPGEAPEQDDGPPGSM
                     VLIPEGDFLMGDNDGSRNERPEHTVWLDAYFIDRYEVSMAEYQKLLDTDYSIEPPPLW
                     DDGIATGEFGIRPAVGISWNDAKRYCEWVEKRLPTEAEWEKAARGTDGRRYPWGHMQP
                     FVDIANYNQGTTGWVSYKLTLSPVTSGTKGMSIRHGLKQGGKSAYGLYNMAGNAAEWV
                     NDWYDRFYYEESPKKNPQGPEEGDYKVLRGGSWEDQPVNLRVTARSKGEVDFQDLTTG
                     FRCAKDAK"
     gene            complement(2486..2833)
                     /locus_tag="PKM72_13815"
     CDS             complement(2486..2833)
                     /locus_tag="PKM72_13815"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNP61918.1"
                     /translation="MKSHPIIDRSTVSKNWHARGFSCGVWIDHSGREWRAVTQDMEEI
                     FMAMSGELELEIAGERVHPSVGEEIHIPGGAPYTIRNIGGTTARWLYGQKRGPSTPIQ
                     PIESLRDLIQTPR"
BASE COUNT          908 a          756 c          910 g          947 t
ORIGIN      
        1 cgtagcaagt cccctcatgt attttccgct ctttgccctg accttttaga aacgcgccac
       61 ccgttttcag aaattaattt ggtatcttcc caattcgttc ataggacgct tctcctatgg
      121 ataatcattt ataggctctt aggaatcgga aattctaagt atggggtatg tcgtgtgtgc
      181 atggagaatg ttgttctgag tcgaatcgtc acagatattc tgttctacac aatacctcac
      241 caatatccgt ttcgccctat tcacctgtaa ttttgaggaa ggcatcaaga aatgccagat
      301 cattccggtg ctcactccga tcaggccgga gaagatttct tcaaagggtt atttcacgtg
      361 ctgtcgttgg ggttgcggcg caagctggaa tcaatttcgt ttctcctcaa gatcttcgcg
      421 cccagaccaa tcttaatccc gatggagctt tacaggaatt actcgcaggc aatcggcggt
      481 tcgctgcaga tcaactcaca tcgattgagc ccgatctggc catcttgaaa gcgcataccg
      541 ttgataaaca agagccattt gcggccatac ttgcctgtgc cgattctcgc atacctgtgg
      601 aactcgtctt tgaccagacg atcggtcaca tcttcgtcac acgtgttgcg gggaatgtgg
      661 tgacaccgca gattatcgcc agcctggaat atggagtggc tgtcctaggg gtcaaagttc
      721 ttgttgtggt tggtcatagt ggctgcgggg cagtaagagc tgcgatgaaa cccgacacgg
      781 taccagaaca aatcagcgtg ttgtatcagc atcttctgcc aaatgggaca gtctggcgat
      841 aatctcgata aagccatcga ggccaatgca aggtttcagg ccgaattgct acgcacgtcc
      901 tctacagtca tacgggatgc tgctaaggcc ggcaaactaa aggttgaagc tggagtttat
      961 gatcttgcca caggcatagt cagtttgagc taaccgcctc aaccctgata ttttcaactg
     1021 aaccggattt ttgcagattg gggtgatggg aatccgtgtg gtcacgaaac ttgaactcgc
     1081 tatttctttg ggaggcactt taaaagcata gttttttggt ctctcttgtc tggctctcct
     1141 aggatcaccg atacttcagt ggggaattgt aatggtgagt actgggaatg tcatgattct
     1201 gtgccaagtc atgtatggaa tattgattct tgagcctaac actcccaatt tttccacatt
     1261 ctgaacaggc cgctttatgc ctagcataag taatcgtcaa gatatttgga aaataccgct
     1321 cctcattgaa tcccgttggg gacgcaaggc tcaaactgtt gaagatttgt tgagaggtat
     1381 tggtttgtta tgcggtatat taggtttcat aagaatgtgg ctttgccccc ttcactcagc
     1441 cactcaagtt cgatatggta aaacacaaga ccagagctta attcttaagt gagtatgcca
     1501 ctaatggtgc gaaacttttt ctgtcctggc ggatggtccc tgacagattc tcgtactctt
     1561 aatcattcct aatctttggc gaggattgta tgacgcgtac atggttttgg ggaattgttg
     1621 gaatcctttt catcataaat ggtctgagcc agcctgcctg gtctcaagag tccaaaccag
     1681 gagaggctcc agagcaggat gatggcccgc cgggttcaat ggtgcttatt cctgaagggg
     1741 attttctcat gggagataat gacggttctc gaaatgagcg accggaacat acggtttggc
     1801 tggatgccta ttttatcgat cggtacgaag tgagcatggc ggaataccag aaattattgg
     1861 ataccgatta tagtattgaa cctccgccgc tgtgggatga tggtattgcc acgggagaat
     1921 ttgggatcag accggctgtg ggaatttcct ggaacgatgc caaacggtac tgtgaatggg
     1981 tggaaaaacg tttgccgaca gaagccgaat gggaaaaagc ggcccgaggc acggatgggc
     2041 gaaggtatcc atggggtcac atgcagccct ttgtggatat tgctaactat aaccagggga
     2101 cgacggggtg ggtgagttat aaactgaccc tttcgccggt tacgagtggg accaagggca
     2161 tgagtattcg tcatggctta aaacagggcg gaaaaagcgc ctatggcttg tataatatgg
     2221 cagggaatgc tgccgaatgg gtcaatgatt ggtatgaccg tttttattat gaagagagtc
     2281 ccaagaagaa tcctcaaggg ccggaggaag gcgattataa agtattacgc gggggctcgt
     2341 gggaggacca gcctgtaaac ctacgggtaa ccgcccgctc aaaaggggaa gttgattttc
     2401 aggatctcac aaccggattt cgttgtgcca aggatgcgaa gtgacggtgc ggctactcgt
     2461 aacagtggcc gcacccggta aacttttacc ttggggtttg gatgagatcg cgaagtgatt
     2521 caatcggttg aatgggagtg gaagggcctc gtttttgccc atagagccaa cgagcggttg
     2581 ttcctccaat attccgaatg gtatagggtg caccgcctgg aatatgaatt tcttctccca
     2641 cggatgggtg aacccgctct cccgctatct ccaattccaa ttctcctgac attgccataa
     2701 aaatttcttc catatcctgc gtgacggctc tccattctcg tccggaatga tcaatccaca
     2761 ccccacaaga aaatccgcga gcatgccaat tcttcgaaac ggttgagcga tcaatgatgg
     2821 ggtggctttt catgataggc ctcttagagg ataaaagact tataaaaaat tccgatgaat
     2881 aagctggcga tcgccgcaat ggagccattg attcctgccg aagaagaagt gcggagatgg
     2941 atgcaagtga caaggacatg cgccagtcac tcgtattgaa ggaaatccat ggaaaagcac
     3001 cggcagtgca ttcatggggg tagaaagaag agaatgaaag agagtgaaat tttctccgaa
     3061 tgcgtccact tatatagagg ggacataccc ctgtcaatgt cacaaattgt ggaaaaggtg
     3121 tgaacgatgg tggataaatt caccgtgaaa tcacgaactc ctttgtgaaa agggaattca
     3181 ctatgtggat tatggggata aatatttttt tgacggcttt gtccaataaa agagtgcgaa
     3241 gccaaataaa atccatcatt gatgtataag gtttgtggtg tcacggtcca tggggcaaca
     3301 ctattgcggc atctaaagta attggggaaa atggctgatt tcctattcag ggcactgtca
     3361 tctctggtgt ggggccctgt ccttcgcgta tatttttaca cgaatatggt ctgtggtccg
     3421 catcgaactt taatctactc ttagaacaag atcatgattg acggattgaa ctattaccaa
     3481 attctgggga ttcctgaaga tgcactcctg aaggaagtgc a
//
LOCUS       DAORAQ010000260         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3726_length_3518_cov_61.610742, whole genome shotgun sequence.
ACCESSION   DAORAQ010000260 DAORAQ010000000
VERSION     DAORAQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_3518_cov_61.610742"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKM72_13820"
     CDS             <1..440
                     /locus_tag="PKM72_13820"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="catalyzes the conversion of dihydroorotate to
                     orotate in the pyrimidine biosynthesis pathway; subclass
                     1A is a dimer formed by two identical PyrD subunits each
                     containing an FMN group; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HNP61919.1"
                     /translation="YFDPIHHQKIAEVLQRSGVAFVTVINSVGNTLVVDPEREMVVIK
                     PKGGFGGLGGRMIKPVALANVRAFWKIWGDRIPIIGTGGVENGVDVFEHVLCGASAVQ
                     IGTVLVEEGCEVFARLEREIADCLQRKGYPSLMACRGKLREMD"
     gene            complement(543..833)
                     /locus_tag="PKM72_13825"
     CDS             complement(543..833)
                     /locus_tag="PKM72_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNP61920.1"
                     /translation="MPKGKVTESESTEELLEPNSEGQSLTARQKEILSLVAQGFTNRE
                     IGEQLDISVRTVEVHRFNLMRRLRVRNVAQLLRQAIALRIIPKGPPTPTTTA"
     gene            906..3485
                     /locus_tag="PKM72_13830"
     CDS             906..3485
                     /locus_tag="PKM72_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNP61921.1"
                     /translation="MIRILLLLVLDHVRHRPFRAFLTVVGVAIGVSAWLAIRLANGEV
                     YQSFEQSVDSVVGKASITLSRGTEGMDEAIIELIQRHPGVKSAHPILKIVVTIQQEGL
                     STGQTLVIWGVDLLNYEKVRLSEDSTPSIPDNRWKQLFSPQTVFLGKEFAKELGMREG
                     QTLTVTGQGVSHDLVVGGLLGSSDALLQGTERQAIMDIAAAQWLFSRLGRLHSIAVVP
                     ELGVGGATLIQALQAVVPPDIRISQSSRRTGQVESMLKAFQFNLTMLSGIALLVGVFL
                     VYNTMAFSVAHHRREIGILRALGMERRAITGLFLLEASLLGIVGGVMGCWFGVFLASG
                     LTTLIGQSVGELYGVTSLAVSQIHPWLLAEAITIGSGVSLLGALRPSWDASTIAPVQA
                     LTVGHSEDEETGSYRTSGWIAIIAFGASVALGSMAPLDGIPAGGYAAAFCLLVGGTAL
                     GPVLCGLIHEGRGTWQSGQWSLLPSLAAEQLSRNPGRTSVTMAAIVVGLSIMVGVGIM
                     IQSFRHTVEIWIEQTMLADIIVAPVSWLGEQEVENNTTGLPLTLVKTVMSIPGVEAVD
                     PYMETMGEVAGESVSLVARDMALHAGRSRYLFVTGDSTPILQETIEDEGVIVSEVLAG
                     RLGLAVGSHLDVKTPLGQQAFPVKGIFYDYATDGGKVVMHDQLFRRHWGETDATIFAV
                     YLKAGQALPIVRREIEQVLKNAIPIVTISNGELKTEILEIFDRTFRVTYVLELIALSV
                     AVLGIINTLMTAIAERRRELATLRALGVSRPQIQGLIFWESCYVAGLGAGLGILVGLA
                     LSVLLINVINKQSFGWTIQFTLPWETLGMAVLVALLAAFLGAWGPARWASRQSIAEDL
                     RYE"
BASE COUNT          737 a          778 c         1099 g          904 t
ORIGIN      
        1 cctactttga tccgatccat catcagaaaa ttgccgaggt gcttcagcga agtggcgtgg
       61 cctttgtgac ggtcatcaat tcagtgggga ataccctggt cgttgatccg gaacgggaaa
      121 tggtggtgat taagcccaaa gggggatttg gcggattggg tggtaggatg attaagcccg
      181 tagccttggc caatgttcgc gcgttttgga agatctgggg tgatcggatt cctattattg
      241 gtacaggcgg ggtggagaat ggagtggatg tgttcgagca tgtgttgtgt ggggcctcag
      301 cagttcagat aggcacggtg ctggttgagg aaggctgcga ggtgtttgca aggctcgaac
      361 gggaaattgc tgactgcttg caacggaaag gctatccgtc gttaatggca tgtcgaggga
      421 aattacggga gatggattga aaaagtacga attttgatct gcctgaatag gtaaagtcaa
      481 aatgagctac agctactgcg ctcttgacat ttttataaca ggataggcga gagatgcctc
      541 atctaggccg ttgtggtcgg ggttggtggg cctttgggaa tgattcgaag cgcaatggct
      601 tgccggagta actgagctac gtttctgacc cgtagacgcc gcatcaagtt gaagcggtgg
      661 acttcgactg ttcgcacgct aatgtctagc tgttcgccaa tttcacgatt agtaaagcct
      721 tgagccacca ggctcaggat ttccttttgt cgggcggtga gagattggcc ctcggaattg
      781 ggttccaaga gttcttctgt tgattcggat tcagtcactt tcccttttgg catattgagt
      841 cacaacccca cgcatatttt tctagtaaat ttaacatacg tcccttactt ccgacaaccg
      901 ctctcatgat acgaatcctt ctcctattgg tccttgacca tgttcgccat cggccttttc
      961 gagcctttct cacggtggtc ggggtagcaa ttggagtctc ggcctggttg gccattcgtc
     1021 tggccaatgg ggaagtctat caatcctttg aacagtcggt agactccgtc gtgggaaagg
     1081 cgtctatcac cctgtcccga ggcacagaag ggatggatga agcgatcatc gagctgattc
     1141 aacgtcatcc tggggtcaaa tcggctcatc ctattcttaa aattgttgtc acaatccaac
     1201 aagaaggcct ctccacgggt cagacgctcg tgatttgggg ggtagatctt ctgaattatg
     1261 agaaagtcag gctatcagag gattcgaccc catcgattcc ggacaatcgg tggaaacagc
     1321 tgttttctcc gcagacggtt tttttaggga aagaatttgc caaagaatta gggatgaggg
     1381 aagggcagac gctgaccgtg acgggtcagg gcgtctccca tgatctggtc gtgggaggcc
     1441 tgttgggatc gtctgatgct cttctgcaag gaaccgaacg acaggcgatc atggatattg
     1501 ctgcagccca atggttgttc agtcggttgg gacgtctgca ttccattgcc gtggttcctg
     1561 agttgggtgt ggggggggcc acgctcatcc aagccttgca ggccgtggtg cctccggata
     1621 ttcgaatcag tcagtcctcg cgacggacgg ggcaagtgga atcgatgttg aaggccttcc
     1681 agtttaatct caccatgctt agtggaatag cgctattagt gggggtgttt ttggtctata
     1741 acacgatggc attttcggtg gcacatcatc gtcgggagat tggcattctt cgggcactcg
     1801 gcatggaacg gcgagccatc acgggattgt ttctcttgga ggcgagtctg ctcggcattg
     1861 tggggggagt catgggctgt tggttcgggg tctttctggc cagcgggttg acgacgctca
     1921 tagggcagag tgttggggaa ttatatggcg tgacatcttt ggctgtctcg cagattcatc
     1981 cctggctgtt agcggaggcg ataacgattg ggtctggggt ttccctcctc ggagcgctcc
     2041 gtccttcctg ggatgccagc acgatagccc cggttcaagc cttaacggta ggccattcag
     2101 aagatgaaga aaccggttcg tacaggacgt cagggtggat cgcgataatc gcatttggtg
     2161 caagtgtggc cttgggttct atggccccgt tggatggcat tccggccgga gggtatgctg
     2221 cggcattttg tttactggtg ggcggaaccg ctctgggacc tgtactctgc gggttaattc
     2281 atgaggggag gggcacatgg caaagcgggc aatggagcct cttgccgtcc cttgcggcag
     2341 aacagctcag ccgcaatccc ggtcggacca gtgtgactat ggcggcgatt gtggtcgggt
     2401 tgtccattat ggttggagtg ggcatcatga ttcaaagttt ccggcacacc gtagaaattt
     2461 ggattgagca aacgatgctg gcggatatca tcgtggctcc ggtgtcatgg ttgggggaac
     2521 aagaggtcga gaataatacc acgggtctgc cgttgactct ggtcaaaacg gtcatgtcga
     2581 taccaggggt cgaggcggtt gatccctaca tggaaaccat gggtgaggtt gcaggggagt
     2641 cggtgtcgtt ggtcgcacgc gacatggccc ttcatgccgg gcgcagccga tatctgtttg
     2701 tgacaggtga ttccactccc attttacaag aaacgatcga agatgaaggc gtgatcgtgt
     2761 ctgaagtgtt agccgggcgg ctggggctgg cggtaggaag ccacttagac gtgaagacgc
     2821 ctttaggaca acaggccttt cccgtaaagg gcatttttta tgattatgcc accgatgggg
     2881 gaaaagtggt catgcacgac caactctttc gtcggcattg gggggagacc gacgcgacca
     2941 tctttgcggt gtatctcaag gccggtcagg cgctcccgat tgtgcgacgt gaaatcgaac
     3001 aggtgttaaa aaatgccatc cccatcgtca cgatcagtaa cggggaactg aagacagaga
     3061 ttctggaaat ttttgatcgg acgtttcgag ttacctatgt gttggagttg attgccctga
     3121 gcgtggccgt gctggggatt atcaataccc tcatgaccgc catcgcggaa cgccggcggg
     3181 aactggccac gctacgagcg ttgggtgtga gtcgcccaca aatccaaggc ctgatttttt
     3241 gggaatcctg ctatgtggct gggttgggtg ccggcttagg aattctggtg ggattggcgc
     3301 tgtcggtgct actgatcaac gtaatcaata agcaatcatt tggctggacc attcagttca
     3361 cactgccatg ggaaacgttg gggatggctg tgctcgtggc gttgttggcg gcgtttctag
     3421 gcgcgtgggg gccggccaga tgggccagcc gtcaatcgat cgccgaggat ttacggtatg
     3481 aatgaaatgt gtgaatggtc gtgctgaaaa aagtctta
//
LOCUS       DAORAQ010000261         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3795_length_3471_cov_49.238583, whole genome shotgun sequence.
ACCESSION   DAORAQ010000261 DAORAQ010000000
VERSION     DAORAQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_3471_cov_49.238583"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1696
                     /locus_tag="PKM72_13835"
     CDS             359..1696
                     /locus_tag="PKM72_13835"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNP61922.1"
                     /translation="MPRNPGNFRRSVRRSIAFRVGMTAILLGAVFAGITAVTERQRME
                     EGVVALARATAAHWNVTVRDQLDQFGLLKPGALQDSLKAFMAGNGEQVLEQGRFVYAR
                     VHDADGLELAQLEDTSFISLEVIRQAVDAAGFRLPESDDHFVVRTGLDGRPYVALQIA
                     LTNKQGKIRAWLDGIFALSATEQARLQANIWRTVLHVLTGILITSLILYPVVSQLLGR
                     LSGLTDRLLDANLETIQVLGGAIAKRDGNTDTHNYRVTVYSIRLAEVVGLDDAQIRAL
                     IKGALLHDVGKLGIPDKVLLKPERLDEVEFEIMKAHVQHGLDITGRAAWLAEAQDVVG
                     AHHEKYNGSGYPGGLARDAIPLNARIFAIADVFDALTSWRPYKEPISSDDAMEILEKG
                     RGEHFDPRLLDLFTGIAREVYDQFGGLKGDDARGELGRIMQRYFRRDTAALMP"
     gene            complement(1864..>3471)
                     /gene="flgK"
                     /locus_tag="PKM72_13840"
     CDS             complement(1864..>3471)
                     /gene="flgK"
                     /locus_tag="PKM72_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNP61923.1"
                     /translation="GIQAHQQGLATTAQNISNINTPGYSRQEVVLETNRPSEGVIGSG
                     VQVANVRRTVDVFLENRLTSENEDLGFLSARTTFLSQADGIVAETESSGLSNGVTEFF
                     NALRDVATNPESPIQRTVLLAKAQTLTQGFVDQAQAFNQIRLDANQEISRHLETINGL
                     AERIASLNDDIFKAESSAREALDLRDQRAVLIKDLAELVDIEQVQLRDGIGINVGGQL
                     LVGGNHANGLTTIPDADNPPFQDVAFVRSDGTAFAISHKIQGGKIGGLLTVRDSDIVA
                     FQDRVDRLAAVLVNEFNQQHQVGFALDGTTNNPFFAALSPPGPVPSDTNGGNALGSSV
                     AITDATLLTFQNYEVQFSSVSAYSIVNTQTGGTVTSGTYASGVAINFDGLDVVLSGTP
                     AAGDFFQVSAHKGAAQRLGLAVTNTDKIAASSTALGVPGNNSNALALVDIHTTRQTML
                     GSLTLNDYQTITLGNVGSAARESNVALTNTTLILDQLQSLRESVSGVSLDEELTNLLS
                     FQRSFEASARLLTVADELLQIVLAMGR"
BASE COUNT          835 a          905 c          923 g          808 t
ORIGIN      
        1 cccactgcca ccaggcgccg cccagccatt atatgatgca tttcaccatg atttcgcaaa
       61 gagtggctgg gcagtcccgg gcccgagtgg agcagtgttt tttatgtcat cagaccactt
      121 catggccgga tatcaaacag gccgggatgt ataaacatca ttaataacga agcgtaagat
      181 tattgagaga gaagggctca ccgggaatac acggccttga tgttcagacg agaatcgaag
      241 aagcatttgc cttactcgac gaatgggcaa ttgaatgctg tctgtggaac agaaattttt
      301 ttaacctttt gatcttgcga gctattgaca acagctgtct ggaaatggtt ctagcttgat
      361 gcccaggaat ccagggaatt tccgccgttc tgttcgccgt tccatcgcat ttcgtgtcgg
      421 catgacggcg atcctgctcg gagctgtttt cgctggaatc accgcggtca cggagcgcca
      481 acgaatggaa gaaggcgtcg tcgcactggc ccgcgcgaca gccgcccatt ggaatgtcac
      541 cgtccgtgac caactcgatc agttcggctt gctgaagcct ggagcactgc aggattcact
      601 gaaggcattc atggctggaa acggcgaaca ggtgcttgag cagggacggt ttgtatacgc
      661 gcgagtgcat gacgccgatg gtctggagct tgctcaactg gaagatacaa gcttcataag
      721 cctggaggtg atccgccaag cggtggacgc ggctggcttc cggctacccg aatcggacga
      781 ccactttgtc gttaggacgg gactcgacgg ccgtccgtac gttgccctac agattgcgtt
      841 aaccaataaa caaggaaaga ttcgcgcatg gcttgatggc atttttgcac tatccgctac
      901 cgagcaggca cgattgcaag ctaacatctg gcgcacggtt ttgcatgtac ttacagggat
      961 tctgatcacc agcctgatac tgtacccagt cgtctcgcaa ctgctcggac gtctaagcgg
     1021 actgaccgac cgcctgctcg acgccaattt ggagactatc caggtgcttg gcggagcgat
     1081 tgcgaaacgt gacggcaaca ccgatacgca taattaccgt gtgacggtgt attcgatccg
     1141 gctggcggag gtggtcggcc tggacgacgc gcaaattcgg gcactgatta agggtgcgct
     1201 gctgcacgat gtcggaaaac tcggcattcc tgacaaagtg ctgctcaagc cagagcgtct
     1261 ggatgaggtg gagtttgaga ttatgaaagc ccacgtgcag cacgggcttg acataaccgg
     1321 ccgggcagcg tggctggccg aagcgcagga tgtcgttggc gcgcatcacg agaagtacaa
     1381 tggcagtgga taccctgggg gtttggccag agacgccata cctcttaacg cacggatttt
     1441 tgctatcgcg gacgtattcg atgccttgac ctcatggcgg ccatacaagg aaccaatctc
     1501 atccgacgac gcaatggaga ttctggaaaa gggacgcggg gagcacttcg acccgcgctt
     1561 gctggacttg ttcacgggga tcgcacgaga ggtgtacgac caattcggtg gcttgaaagg
     1621 cgacgacgca cgtggggaac tcggccgcat catgcaacgt tattttcgcc gagatacggc
     1681 cgcattgatg ccttagccgc atcggtggtt gtacaatagg gttgaggctt gacacccaaa
     1741 gggcaatgga tggagatgga atcatggtga gacattcgta tcctatcgga ctttcctggc
     1801 ttgtgagatt ttcacacaag attggtgaaa gattcgtgaa gagtctcctt gtcatttgac
     1861 cttctatcga cccatcgcca agacgatttg aagtaattca tcagcgacgg taagcaatcg
     1921 ggcggaagcc tcaaacgaac gttgaaaact caacagattc gtgagttctt catctaaaga
     1981 cactcctgac acgctttccc gtaaactttg aagttggtcg agaatgagcg tcgtattggt
     2041 caaagccaca ttcgattccc gcgctgcgct gcccacattc cctaacgtaa tcgtctgata
     2101 atcgtttaac gtcaaactcc cgagcatggt ctggcgagtc gtatggatat caacgagtgc
     2161 caacgcattg gaattattgc cgggaacacc aagggctgtt gaggaagccg caattttatc
     2221 ggtattcgtt acagccaggc ccaaacgttg cgccgcgccc ttatgcgcac tcacctggaa
     2281 aaaatcgcct gctgcgggtg tgccgctcaa caccacatcc agtccatcaa agttaatggc
     2341 cacacctgat gcgtaagttc ccgacgtgac cgtccctccc gtctgcgtat tcacaatgga
     2401 ataggcagaa acacttgaaa attgcacctc gtaattctgg aaggtcagaa gagtcgcatc
     2461 tgtaatcgca acggaactgc ccaaggcatt tccaccattg gtatcacttg gaacagggcc
     2521 tggaggggat aatgctgcga aaaaggggtt attggttgtt ccatctaacg caaaccccac
     2581 ctgatgctgc tggttaaatt cattcacgag gaccgccgct aaccgatcga ctcgatcctg
     2641 gaaggccaca atatcggaat cccggactgt cagcagtccc ccaatctttc ccccttgaat
     2701 tttgtgagaa atggcaaatg ccgtgccgtc actgcgcaca aaagccacat cctgaaatgg
     2761 gggattatcg gcatccggaa tcgtggtcag cccattagca tgattcccgc caaccaataa
     2821 ttggcctcca acattaatgc caatcccatc gcgtaattgc acttgttcga tatcgaccaa
     2881 ttccgccaaa tccttaatca acacggctcg ctggtctcgt aaatccaggg cttcacgcgc
     2941 actggattcc gctttaaaaa tgtcatcgtt cagtgaagca attcgctcag ccaggccatt
     3001 aatggtttca agatgtcgac tgatttcttg attggcgtct aaacggattt gattgaacgc
     3061 ttgggcttga tccacaaagc cttgggttaa ggtttgtgcc ttggcgagaa gcaccgttcg
     3121 ctgaatggga ctttccggat tggtcgccac atcccggagg gcattaaaaa attccgtcac
     3181 accattggaa agcccagaac tctccgtttc agccacgatg ccatcggcct gagataagaa
     3241 cgtggtgcgc gccgaaagga atccaaggtc ttcattttcg ctggttaatc gattttctag
     3301 aaaaacatcg accgtccgtc ggacattggc gacttgcacc ccgctcccaa tgaccccttc
     3361 agaggggcga ttggtctcta acaccacttc ctgacgagag tagcctgggg tattgatgtt
     3421 ggaaatattc tgggcagtcg tggccagccc ttgctggtgg gcctgtatcc c
//
LOCUS       DAORAQ010000262         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3808_length_3461_cov_71.949501, whole genome shotgun sequence.
ACCESSION   DAORAQ010000262 DAORAQ010000000
VERSION     DAORAQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3808_length_3461_cov_71.949501"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKM72_13845"
     CDS             complement(<1..226)
                     /locus_tag="PKM72_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNP61924.1"
                     /translation="MPLSATEYHSLTRIAIQAARQGGAILLDYAKKGFQVHQKDQAIN
                     LVTEADLRSEEAVIQTIRQAFPEHQILSEEQ"
     gene            353..550
                     /locus_tag="PKM72_13850"
     CDS             353..550
                     /locus_tag="PKM72_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61925.1"
                     /translation="MGGPTPSGTAGGLYSYFFLVFAFALGFAAAAAFFFGAAFFFVAA
                     NSLTPFQIIDHARHCGSDGFG"
     gene            complement(533..1057)
                     /locus_tag="PKM72_13855"
     CDS             complement(533..1057)
                     /locus_tag="PKM72_13855"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNP61926.1"
                     /translation="MEDQNDQLKDISDGLEQRISVYERKLKDLQKKREQVGEEIATVE
                     KYLELAKTLYRVEADKAKLASLSSQIFSNKEGHLSSADTDVASKSREILLGRSKYVGM
                     SVPDAVYMVLQEIGRPLHAKELFQRLKEGGMPIRGKTPVTSVATSLKRDPRFRKVGPN
                     TFEVNHEKTLTKAV"
     gene            1797..2900
                     /gene="rlmN"
                     /locus_tag="PKM72_13860"
     CDS             1797..2900
                     /gene="rlmN"
                     /locus_tag="PKM72_13860"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNP61927.1"
                     /translation="MTANTPPLNLLALNANELSAFIQELGWPRYRAGQILRWLYQHRV
                     VDIESMTNLSKTDRGRLQEVATIHRMESLPPTIASDGTVKFVWNLEDGLAVETILIPD
                     GRRRTLCLSSQVGCTLDCGFCLTAEMGLKRSLRAHEIVGQVLNVQAYLPEDEHLSSLV
                     FMGMGEPLVNFEPVADAIQRLTNIEWGLGFSPRRITVSTAGWIPRFPDVRRLGVNLAI
                     SLNGTTNEQRGQLMPAVNGRYDLSELLDACRQYTQFSERPLTFEYVLLAGVNDSPEDA
                     RRLVRLVQGIRCKVNLIPFNEFPGNPFRRPSPGVVDAFQDIVRNKGLDVFLRKSRGDD
                     VLGACGQLGRSASEVLNQTTTNKKRSLPLYAQP"
     gene            2887..>3461
                     /gene="larE"
                     /locus_tag="PKM72_13865"
     CDS             2887..>3461
                     /gene="larE"
                     /locus_tag="PKM72_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNP61928.1"
                     /translation="MLSLEPTLQGKYEALQELFSDMESVVVAFSGGVDSTLVLKVGFD
                     ILGSQARAVTAVSPTFPALEVEEVKQLSAAIGAPLQLVETDQLQIESFVKNDATRCFH
                     CKTDLYRLLSSLRDAVGFHTIVDGTNVDDLGEDRPGINAARQLGVRSPLVEAGLSKAD
                     IRELAKGLGLANWNKPAAACLSSRITRGLPIT"
BASE COUNT          800 a          736 c          914 g         1011 t
ORIGIN      
        1 cctgttcttc gctgaggatc tggtgttcgg gaaacgcctg tcggatggtc tgaatgacgg
       61 cttcctctga acgcaggtcc gcttcggtga ccaggttgat ggcttgatcc ttttgatgga
      121 cttgaaatcc ttttttggcg tagtcgagaa gaatcgctcc gccctgccgg gcggcttgaa
      181 tggcgatgcg ggttaacgag tgatattcgg tagcagatag tggcatagat aatgtgggaa
      241 agaacgtttt ccagcatacc tgaagtctgc ggaaatccca agcgtcaggt tacgttcatg
      301 cgaatcagtg atgtgagtgt ttaagggagt tagaaggaaa aacacactgg gagtgggtgg
      361 ccccactccc agtggcactg caggtgggct ctacagctac ttcttcttgg tctttgcttt
      421 tgctttgggt ttcgcggcgg ccgcggcctt tttctttgga gcggctttct tttttgttgc
      481 tgccaattca ctcaccccct ttcagatcat cgatcatgcc cgtcattgcg ggtcagacgg
      541 ctttggttaa tgttttctcg tgattgactt caaatgtatt gggccccact tttcgaaacc
      601 gaggatcgcg ttttaaggat gtggcaactg atgtgactgg agtttttccc cggatcggca
      661 tcccgccttc tttgaggcgt tgaaaaagtt ctttggcatg tagtggacga ccgatctctt
      721 ggagaaccat gtacactgca tcaggtacgc tcatacccac gtatttgctt cggcctagga
      781 gaatttccct cgacttagac gcgacatccg tatcggctga ggacagatgc ccttctttat
      841 tcgagaagat ctggctggag agactcgcca gcttcgcttt gtcagcttcg acgcgataaa
      901 gggtttttgc caactccagg tatttctcaa ccgtcgcgat ttcctcacct acctgctccc
      961 gttttttttg aagatccttg agttttcgct cataaacact aatgcgctgt tccagcccat
     1021 cactgatatc tttaagctgg tcattttgat cctccatttc gcgaactcca ccatatgttg
     1081 agtctaattc tattcgtgtt ttaaagcaaa gtcaacaagc aagctttaaa tttttattat
     1141 taaaggttgc cttcaaacaa atatgcaaat tgcgattggt ttattataag tcaatggtct
     1201 gtttgtgaag tgataattat gaaatttatc tattaaaata aaaagcctaa acttagtggt
     1261 ttatgattct gtgaattcaa catttggtag gatagtcgtt cttgcagtat gtgaatgtgc
     1321 cttattaact aggtattgta tcgtggtatt ggcgtttgga atggttggtt tgagagtgat
     1381 gataattggc ataaatttct acaataatga gcgataatgt aagcggctca ttttttgacg
     1441 gaatttaagt cagaaaatat gctatggagt aaaccgttgt ttaaagcatt tcatgaatga
     1501 gttaacaaca tattgtggtg tagtcctacc taggaaaatt tctgaattgg gaggaatcgg
     1561 ctgtaaaggt ttctaagcgg tcaaaatttc tccgttttct tgaaccatcc acgatgggcc
     1621 agtgaaatcg tcaaacttcg gctttgctca gtcgccattt tgctctgatc accccggaca
     1681 gacaggctct aggtgtaaac cgagcctatt gagacaggct ggaatatttt taagatggct
     1741 tgggaagata aggatgctat gattgatgaa ttatttgatt gttggactta aacgtgatga
     1801 ctgcaaatac cccccctctc aatcttcttg ccctcaacgc aaatgaactg agtgccttca
     1861 tacaggaact cggttggcca cgatatcggg caggtcaaat tctgcgatgg ctctaccagc
     1921 atcgtgtagt ggatattgag tcgatgacca acttgtctaa gaccgatcgg ggcaggttgc
     1981 aggaggtagc gactattcac cgtatggaat ctcttcctcc cacgattgcc agcgatggca
     2041 cagttaagtt tgtatggaat ttagaggatg gcttggctgt agagacgata ttgattccgg
     2101 atggccgtcg gcggaccttg tgtctgtctt cccaagtcgg gtgcaccctg gattgtggtt
     2161 tttgtttgac ggccgagatg ggcctgaagc gaagcttgcg cgcccatgaa attgtcgggc
     2221 aggtgttgaa tgtccaagct tatctgccgg aagatgagca tctgtcttca ctggtgttca
     2281 tgggaatggg agagccgttg gtgaattttg agccggtcgc cgatgcgatc cagcgtctca
     2341 ccaatattga gtggggcttg ggtttttccc cacgacgcat cacggtgtcc acggcgggct
     2401 ggatcccgcg atttccagat gtgagacgat taggcgtgaa tctggccata tccttaaatg
     2461 gcacgaccaa tgaacaacgg ggacaactga tgcctgcagt gaatgggcgc tacgatttat
     2521 ctgaattgct tgatgcctgt cgtcaatata ctcaattcag cgaacgccct ctgacgtttg
     2581 aatatgtgtt gttagcgggc gtcaatgatt cacctgaaga tgccagacgt ttggtacggc
     2641 ttgtacaggg gatccggtgt aaggtgaatt tgattccgtt taatgagttt ccgggtaacc
     2701 cctttcgacg tccgtcacca ggtgtggttg atgcctttca agatattgtt agaaataaag
     2761 gcctggatgt gtttctccga aaaagccggg gagacgatgt gttgggagct tgcggccaat
     2821 taggtcgatc agcttccgaa gtcttaaatc agacgactac gaacaagaaa aggagtctcc
     2881 ctctctatgc tcagccttga accaactctg caggggaagt atgaggcctt gcaagaactc
     2941 ttttccgata tggaatcggt ggtggttgca ttttctggcg gggtcgatag cacactcgtt
     3001 ttaaaggttg gatttgacat acttgggtct caggccaggg ccgtgacggc ggtttcgcca
     3061 acatttccgg cactggaagt ggaagaggtc aaacaattaa gtgcagcgat cggcgcgcct
     3121 ttgcagttgg tcgaaacgga tcagttgcaa attgagtcat ttgtcaaaaa tgatgcaacc
     3181 cgatgtttcc attgcaaaac ggatttatac cgattgcttt ccagtcttcg ggatgccgtg
     3241 gggtttcata ccattgtgga tgggaccaat gtggatgact tgggtgaaga tcgcccaggc
     3301 attaatgcgg ccagacagct aggtgtgcgg agtcctttgg ttgaggcggg actcagtaag
     3361 gcggatattc gggaattggc caaagggttg gggctagcca attggaataa gccggccgcc
     3421 gcttgtttgt cttcacgaat tacccgtggg ctccccatta c
//
LOCUS       DAORAQ010000263         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3809_length_3461_cov_69.500294, whole genome shotgun sequence.
ACCESSION   DAORAQ010000263 DAORAQ010000000
VERSION     DAORAQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3809_length_3461_cov_69.500294"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1702)
                     /locus_tag="PKM72_13870"
     CDS             complement(<1..1702)
                     /locus_tag="PKM72_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNP61929.1"
                     /translation="MPLLPKSHRILERPLMEFRPFGKDTQGATIQDVSGIAIRANLEY
                     LEEAIKQRQGPEAAEAALGTLVALLNERIPDRTYHVTVDFLKNHWNSYSYEFAMFLAE
                     FSVQLSHEENFHFNLGREKFLSPIIQILGRPFSIAQIYRLFPHFVEKFTKGSLKPEVV
                     SVTNGHAVMRLQLSESTIKQFGPYLQGCAERICHTTKATVAEVPARMFGKRAATIQDR
                     SCIAEGAPYCEWAFTWDPERRTMRGWIVGGLALGLATVALLRVFAPEQPWWISAGLSL
                     LPLLTLPLAKRLWDDRLELQERGKIIQEQLESAEQRHEELREAYLAQEHTLIEIRRRV
                     DELTMLHQLTLHIGSTLDRETIIGSGLKAIVGSLSFEHAWAAVWDAPHQLFHKIQSEG
                     MSAQWNTLTHNTHIPSAPQDLLHRTLHTKEPIVIEDITDILSQCHPITRQILAQAGTK
                     SGFSVPLISQNQPLGVLIIGSTHRKPIPITEQNLLSTVAHEMAIALDTALAYDEIEAL
                     NIGLESKVQERTLALQQANTDLESANHRLKELDRMKSQFLSHCSHELRTPLTSIKGFT
                     E"
     gene            complement(1796..3172)
                     /locus_tag="PKM72_13875"
     CDS             complement(1796..3172)
                     /locus_tag="PKM72_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNP61930.1"
                     /translation="MTTLIYCRLVVFCLFLGLLNTFIIPQTIHAEGFRILDHSASATG
                     QGAAFTAQADDASALHYNPAGMTQLKGIQFYAGTLLIGGTVRFKSDLGPNVKGGVGGT
                     IANPPPSTLFLTANLPALGFDNLPNWTVGIGVTTPYALKIEYPDDSLISPILTSASLP
                     LIDIKPTLAYKVNDYIAVGGGLDIYTFASFIGEGQAELQQNAAPGNAFGIPAGTGLEA
                     NGSDTALGFNASVLWTPFRNQDGHPLVNLAFVYRNGPNLDLKGDFLAGGTRLAGAATN
                     IELPDIYTWAIAAWPIRNAQHEWKVEVDLDYADWSGFKDLNLQLSNGAAIQNPRDYGD
                     AWVLMVGTEFKALSPSALPHWDVAFRTGYVRSESPIPDRTFEPAVPDSDFNAFSAGMG
                     FLCHGPGKFLGILPCDIFGAKAIGVDLAYQALLYDERNVQNNQQPFLNGKWDTTLHVG
                     AVSLRMNF"
BASE COUNT          842 a          799 c          996 g          824 t
ORIGIN      
        1 tttccgtgaa tcctttaatc gaggttaagg gtgtgcgcaa ttcatgagag caatgcgaga
       61 ggaattggga tttcatccgg tcaagttcct tgagccggtg gttggcggat tccaagtctg
      121 tgttggcctg ttgtaatgcc agtgttcgct cctgcacttt gctttctaac ccgatattca
      181 aggcttcaat ttcgtcatag gccaatgccg tatcaagggc aatcgccatt tcgtgcgcga
      241 ccgtggataa taagttttgt tcggtgatgg gtatgggttt ccggtgggta ctaccgatga
      301 tcagcacacc aagtggttga ttctgactaa tcaggggaac cgagaatccg cttttcgtcc
      361 cggcttgcgc caatatctgt cgagtgatgg gatggcactg actcagaata tccgtgatgt
      421 cctcaatgac gatgggttct ttcgtgtgga gggttcggtg aaggaggtcc tgtggtgctg
      481 agggaatgtg ggtgttgtga gtcaaggtat tccactgtgc cgacatgcct tctgattgaa
      541 tcttgtgaaa gagttgatgt ggtgcgtccc atacggccgc ccaggcatgc tcaaaggaga
      601 gggagcctac aatggctttg agtcctgatc caataatggt ttcccggtcc agggttgagc
      661 cgatgtggag ggtgagttga tgcagcatgg ttaattcatc gactcgtcgt cggatctcga
      721 tcaaggtatg ctcttgtgcg agataggcct cccgtaattc ttcgtgccgt tgttcagccg
      781 actccaattg ttcttgaatg attttcccgc gctcttgaag ctctagtcgg tcatcccata
      841 gccgttttgc caagggaagg gtgagcaagg ggagcaggga gagtccggca ctgatccacc
      901 agggctgctc gggagcaaac accctcaaga gcgcgaccgt ggcaagcccc aatgcgaggc
      961 ctcccacaat ccatccccgc atggtccggc gctcggggtc ccaggtaaag gcccattcgc
     1021 aatagggggc accctcggcg atacagctcc gatcctggat ggtggcagcc cgtttaccaa
     1081 acatgcgggc cgggacttct gcaacagttg cctttgtggt gtggcaaatt cgttcagcac
     1141 agccctgcag atacggtccg aactgtttga tcgtggattc ggataattgt agccgcatca
     1201 cggcatggcc attggtgacc gatacgacct ccggcttgag cgaacctttg gtgaattttt
     1261 ccacaaagtg ggggaagagg cggtagatct gggctatgga aaagggtcgc ccgaggattt
     1321 gaataatggg cgagagaaat ttttcccgtc ccagattaaa gtggaaattc tcttcatgcg
     1381 agagctgaac agaaaattcg gccaaaaaca tggcaaactc ataggaatag ctgttccagt
     1441 ggttcttgag gaaatcgacg gtcacatgat aggtccggtc gggaattcgc tcgttcagca
     1501 aggcgacaag agtgcccaac gccgcctctg ccgcctcggg tccctgacgt tgctttatgg
     1561 cctcttcaag atattcaaga ttggcgcgaa tagcgatccc gctcacatcc tgaatggttg
     1621 cgccctgagt atccttcccg aatggacgaa actccatgag tggacgttca agaatgcgat
     1681 gggattttgg caagagcggc ataagcatcc cttataaaga aggtaaaccg gtagagggac
     1741 tgtaatggaa tcggagaaaa tggtgaaggg gggacaaaaa ttcagggaag aggcattaga
     1801 aattcattcg cagggacacg gctccgacgt ggagggtggt atcccattta ccgtttaaga
     1861 aaggttgttg gttattctga acattccgtt catcatacag taacgcttga taggctaaat
     1921 ccaccccgat ggctttcgcc ccaaaaatgt cacaggggag tatccccaaa aatttccctg
     1981 gaccatggca gagaaatccc atcccggctg aaaaggcatt gaagtcggaa tctggaaccg
     2041 caggctcgaa tgtccggtcg ggaataggac tttccgatct gacgtatccc gttcgaaagg
     2101 ccacatccca atgggggagc gcagaggggg agagggcttt gaattccgtt cccaccataa
     2161 ggacccaggc atctccatag tcgcggggat tttggatggc agctccatta gagagttgca
     2221 gattgaggtc tttgaaccct gaccaatccg catagtcgag atcgacttcg accttccact
     2281 catgttgcgc gttccgaata ggccatgcgg cgatggccca cgtgtaaata tcggggagct
     2341 caatgtttgt ggcggcaccg gcgagtcggg ttcctccggc taaaaaatct cctttgaggt
     2401 ccaaattggg tccgttgcga tagacaaatg cgagattgac gagtggatga ccgtcctgat
     2461 tacggaaagg tgtccaaaga acactggcgt taaatcccaa ggcggtgtcg gacccgttcg
     2521 cttcaagacc tgttccagcg ggtataccaa acgcattgcc tggtgccgca ttttgttgaa
     2581 gttctgcctg accttctcca ataaagctgg caaatgtata aatatccagc ccgcctccaa
     2641 cagcaatata gtcgttgacc ttgtaggcca gagtgggctt gatatcgata aggggaaggg
     2701 acgcactcgt gagtatggga gaaatcaggc tatcatcggg atattcgatt ttcagggcat
     2761 acggggtggt caccccgatt cctacggtcc aattcgggag attgtcgaag cctaatgcag
     2821 ggaggttcgc ggtgagaaac aaggtgctgg gtgggggatt ggcgatggtc ccaccaaccc
     2881 cacccttcac gtttggtccc aggtcgcttt taaatcgaac cgtacctccg atcaagaggg
     2941 tccccgcata aaattgaatg cctttcaatt gggtcatgcc cgctgggtta tagtgaaggg
     3001 cagacgcatc atcggcttgg gcagtgaagg cagccccctg gcccgtagcg gaagcgctat
     3061 gatcgaggat acgaaatcct tcagcatgta tggtttgggg aatgatgaaa gtattgagga
     3121 gcccaaggaa gaggcaaaaa accacaagcc ggcaatagat caatgtcgtc acgtctcctc
     3181 cttgagaacg gaaaccgatc agaaattccg ggaagtctat gaaaaacaag agaagatagc
     3241 aatcttgatc agggatgagc aacgagtaaa atagcgaaaa gtgttgcctc ctgaccggtg
     3301 atcgcggatt tcctctctat tttgagagga aattcgatgt atgcaggggg tttttgttca
     3361 tgaagtcggg tgagtaaata ttgaaaagga gaatggcatg cgggtaaaaa aactgttgca
     3421 tacgcgaatg cgggtgagtg acatggagca gacgctgggc t
//
LOCUS       DAORAQ010000264         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3846_length_3426_cov_64.539899, whole genome shotgun sequence.
ACCESSION   DAORAQ010000264 DAORAQ010000000
VERSION     DAORAQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_3426_cov_64.539899"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..849)
                     /locus_tag="PKM72_13880"
     CDS             complement(127..849)
                     /locus_tag="PKM72_13880"
                     /inference="COORDINATES: protein motif:HMM:NF037471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61931.1"
                     /translation="MASFNTVLSTHFPKAIPQEDFVKRSYDLLQAEGFRAENTIAFVS
                     LCRDELTLPFVEDVKKTWGEAFIFSSLGGMLFLGKTGFLAAQHHAPNEDGRERYVYFA
                     LPHIGVDTQGEIGRHDRPGRLQPSHACGALHGFQAELANRCVSLGIDLDDIEQSLLKQ
                     HLFRKLTYGDIPDLLSLTKTAYAVILEELERMIQLTVDPVQNDYAVLTGIQIHGPQGM
                     EYIWPGETYWVRDGNRHTIVLE"
     gene            complement(862..1929)
                     /gene="cax"
                     /locus_tag="PKM72_13885"
     CDS             complement(862..1929)
                     /gene="cax"
                     /locus_tag="PKM72_13885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HNP61932.1"
                     /translation="MGLNLLLVFIPIAIGLDWYEANPILVFIVSGLAIVPLAGLMGRS
                     TENLSVYIGATLGGLLAATMGNAPELIISIFALKAGLYDVVKASITGSILGNLLLGMG
                     LAILLGGLRHKQQKFNATAAGMSSGLLTLAGVGLIVPALFHFTSKQETELSTEIAVVL
                     FIAYLLSLVFTLKTHQHLFRTEAEEPIEGSAPQKEEEKEEVWSKNKAIGMLAAVTVGI
                     AFMSEILTGAIEPAAAGIGLTPIFAGVIFLAVFGNAAELMNSVRFARNNQMDLVMGIA
                     VGASIQVVMFVAPMLVFVSYLLGPKPLDLLFVPFEIAAIVIAILVNGNLTKDGESHWM
                     EGVMLLGVYVILGIGFFFLPM"
     gene            complement(2081..3070)
                     /locus_tag="PKM72_13890"
     CDS             complement(2081..3070)
                     /locus_tag="PKM72_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61933.1"
                     /translation="MNIIFYVGGFLCAVLIILWGCSVFTNAVEWLGKRTSTSEGAVGS
                     IYAALGTTLPETAIPVSAFFLTAGAPKTDVGIGAILGAPFTQSTLILPILAILLLVFS
                     RYGRRPPTFKLNVLAVRTDLRCFLLAFSLGIGCAFLPYRWLHLIGAVFLIGLYVYYVV
                     KKLSGQSGGDFNPVPLIFARKTTLPTYGIILLQGVVGVTTLILGSQIFVYVAENLAST
                     LAVSPLFLTLLIAPLATELPELANSVLWLQHRKDTLAISIITGAMVFQGTIPVAIGLV
                     GTDWNLDSQALTPMILALLAMAVCYVHLKWLGHWRPWLLSGSAILYFGFLFSL"
BASE COUNT          946 a          844 c          845 g          791 t
ORIGIN      
        1 ttaaaatcaa attttttgca agggattttt catgaaatag gtgaggtttt cagggaatta
       61 cacaatccat attccaaatt cgtcactaag gtattagcac ttaggtgttt gccgggcgca
      121 ttcactctac tccaaaacaa tggtatgtcg atttccgtcg cgaacccagt aggtctctcc
      181 aggccagata tactccatgc cctggggccc atgaatttgg atgcccgtga gaactgcata
      241 gtcattctgc acgggatcaa cggttaattg gatcattctt tcaagttcct caagaatgac
      301 agcatacgcg gttttcgtca gactcagcag gtccggaatg tcaccgtagg tgagtttgcg
      361 aaagagatgt tgcttcagca ggctttgttc gatgtcatca agatcgattc ccaaagagac
      421 gcaccgattg gccagttcag cttggaatcc gtgcaacgcc ccgcaggcat gggacggttg
      481 caaacgcccg ggacgatcat ggcgaccaat ttcgccttgt gtatccacgc cgatgtgcgg
      541 cagggcaaaa tacacatagc gttcacgccc atcttcattc ggcgcatggt gttgcgcggc
      601 taaaaatccg gtctttccca gaaagagcat gccgccaaga ctggaaaaaa taaaggcttc
      661 tccccaggtt tttttcacgt cctcgacgaa gggcaatgtc agttcatccc ggcacaggct
      721 gacgaaggca atggtgtttt cagcccggaa gccttcagcc tgcaagagat cataagagcg
      781 tttgacaaaa tcctcttggg gtatcgcctt cggaaagtga gtggagagga ctgtgttaaa
      841 gcttgccatg gatttgcctg gctacatcgg gagaaagaaa aatccaatgc ccaaaattac
      901 atagactccc aataacatca cgccttccat ccagtgggat tcgccatctt tggtgaggtt
      961 cccgttgacg agaatcgcga tgacaatggc cgcaatttca aagggcacga aaagcaaatc
     1021 caagggtttg gggccgagaa ggtaactgac gaataccagc atcggcgcca caaacatcac
     1081 cacctgaata ctcgccccga cggcaatgcc catgaccaga tccatttgat tattcctggc
     1141 gaaccgcacc gagttcatca gctctgccgc attcccgaat acggccagaa aaatcacacc
     1201 ggcaaagatg ggagtgaggc cgatgcctgc ggcggcgggt tcaatcgctc cggttaatat
     1261 ttcgctcatg aaggcaatgc ccactgtcac ggcggccaac attccaatgg ctttgttttt
     1321 actccatacc tcttcttttt cttcctcttt ttgtggtgcg ctcccttcga tcggttcttc
     1381 ggcttccgtg cgaaataaat gctgatgagt tttcagggta aataccaggc ttaggaggta
     1441 ggcgatgaag agcacaacgg cgatttcggt actgagttct gtttcctgtt tggaagtgaa
     1501 atgaaacaga gcagggacga tgagtccgac acctgcgagg gttaagagtc ccgaactcat
     1561 cccggccgcc gtcgcattga atttttgttg tttatggcga agaccgccga ggagtatcgc
     1621 aagccccatg ccgagcaaca ggtttcccaa aatggatccc gtgattgagg ctttgaccac
     1681 atcgtacagc cccgctttca aggcaaagat cgaaataatc aattccgggg catttcccat
     1741 tgtggcggcg agtaatcctc ccagggtggc cccgatgtag accgacaggt tttccgtcga
     1801 tcgccccatc aacccggcta agggcacaat ggcgaggccc gagacgatga agacgagaat
     1861 ggggttggct tcataccagt ccagtcctat tgcaatgggg atgaagacca ggagtaaatt
     1921 aagtcccatc gttgccctct tattgaagtg gttaagggaa cccgaaattt cggcagtgta
     1981 cttaatacct accacactat tcattacctg tctgtataaa agtattttcg cgcgcgagaa
     2041 ttgaaacctc cttgagggcg ggatgtaatg gacctccaac tcacaaagag aaaaggaatc
     2101 cgaaatagag tatggcgctt ccgctcaaca gccatggccg ccaatgtcca agccatttca
     2161 aatggacata acatacggcc atagccagga gcgccaatat cattggcgtc agagcttgag
     2221 agtccaggtt ccagtcggtc cccaccagtc ctatggccac aggaatggtc ccctgaaaca
     2281 ccatggcccc ggtgataata ctaatcgcga gggtatcttt tcggtgttgg agccagagca
     2341 cactattggc taattccggc aactccgtgg ccagtggggc aattaataag gtgagaaaaa
     2401 gtggtgagac cgctaaggtc gatgcgagat tctccgccac gtacacgaag atttgagagc
     2461 ctaaaatcaa ggttgtgacg ccaaccactc cttgcaaaag aatgatcccg taagttggga
     2521 gtgtggtctt tcgtgcaaaa atcaggggaa cgggattgaa atctccaccg gactgaccgg
     2581 aaagcttttt tacgacatag taaacataca gaccaattaa aaatactgcg ccaatcaggt
     2641 gcagccagcg ataagggaga aaggcgcaac caattcccaa actaaatgcc aggaggaaac
     2701 aacgcaaatc ggttcgtacc gctaggacat tcagcttaaa ggttggtgga cgtctgccgt
     2761 atcgagagaa caccaaaagc aggatcgcca ggattggcaa aatcagcgta ctctgggtga
     2821 agggagcacc aagaatcgcg ccaattccca catctgtttt gggagcaccc gcagttaaaa
     2881 agaatgcact aacaggaatg gccgtttccg gtagagtcgt acccaacgcc gcataaatgc
     2941 tacccacggc accttctgaa gtgctggtcc gtttccccag ccattcaaca gcattggtaa
     3001 aaacggaaca accccaaagg atgatcaata ccgcgcatag gaaaccaccc acataaaaaa
     3061 taatgttcat gattcaacgc ggaaacgata ttcggattat gtgtaagggg agaaaacggt
     3121 aggagtaaag gtccgcccgc gactataaat aattcaattt ttcaatgaca aaaaagggat
     3181 gatagataac aaaagtgcgt ggggagtagc aagtgtttcc agcacaaaaa aaaatccatg
     3241 aatttttatg cctgaaaatc tgaggcatga actcccagac agcatctcat gaagggggat
     3301 catgttgagt gtcgaaaatg gaagaccttt tacacttttc tttcgggtaa atcgtgatct
     3361 ctcttcttcg tcattcccga caacctttgc tatctaggct ttcctttgga tgggtacccg
     3421 cctgaa
//
LOCUS       DAORAQ010000265         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3852_length_3424_cov_52.970021, whole genome shotgun sequence.
ACCESSION   DAORAQ010000265 DAORAQ010000000
VERSION     DAORAQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3852_length_3424_cov_52.970021"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PKM72_13895"
     CDS             complement(<1..1247)
                     /locus_tag="PKM72_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNP61934.1"
                     /translation="MKTSGPHNLLIGMLLGISTGVLLGGLFPSFGLGIQFLGDLFLQA
                     LFALVVPLVVSSMIVGIASLGDVRQLGPLGIRTVLFFMTTTGLAVLVGLILVIVLHPG
                     VPLEQLPNGTAPAPPSEVSLQMKDQPTSLVELFKTILTSLVPKNLFAAMAETQILPLI
                     VFALIFGGVLTTIGEKGILVIRLFEGINDAMMAIVHLLMWVAPVGIGALIAGRLGEAG
                     GFSGFWPQLSSLGTYVATVLIALGIHGFLILPLALRILGKQSVPSYAKAMTTPLMTAF
                     STSSSSATLPLTMESLIEEAGVSRRVVSFVVPLGATINMNGTALYEAVAAMFIAQTYG
                     IELGLGETLIVLITATLSAIGAAGIPEAGLVTMVIVLKAVDLPIEGISLILVVDWFLD
                     RCRTAVNVWGDAVGAAVVDRWESG"
     gene            complement(1314..>3424)
                     /gene="gcvP"
                     /locus_tag="PKM72_13900"
     CDS             complement(1314..>3424)
                     /gene="gcvP"
                     /locus_tag="PKM72_13900"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNP61935.1"
                     /translation="GEWGADIAVGSSQRFGVPLGYGGPHAAFLATKEAFKRHIPGRMV
                     GVSKDAHGQKAYRLALQTREQHIRRDRATSNICTAQVLLANIAGMYAVYHGPEGLKSI
                     ANKVHHLTTLTAEGLRRLGCQLHSNDFFDTLRVRCPHLSQAKIIKNGQQKFINLRQYA
                     DGSFGIALDEATTITDVSQLLTLFTDKESLPFSLSDLADQLGSSIPTTFKRQSPFLTH
                     PVFHDYHSEHELLRYMYLLQSKDLSLVHSMIPLGSCTMKLNATAEMVPVTWPEFSRLH
                     PFAPSNQAQGYQSLFQQLEAWLAEITGFEAVSLQPNAGSQGEYAGLMVIRAYQEERGQ
                     EQRNICLIPVSAHGTNPASAVIAGLKVVPVACDNHGNIDLHDLEAKSAQYHDTLACLM
                     VTYPSTHGVFEESIRKVCEIVHAHEGQVYLDGANMNAMVGLVRPGAIGADVCHLNLHK
                     TFCIPHGGGGPGMGPIAVAPHLAPFLPGHPLGQVGGSKGIGPIAAAPYGSPNILPISW
                     VFIKLMGSEGLTKATQVAILNANYMASRLIKYYPVLFTGKNGMAAHEFILDLRPFKKS
                     AEVEVEDVAKRLMDFGFHAPTISWPVPGTLMIEPTESESKVELDRYCDALILIRQEIA
                     EIEEGRLPRDNNPLKHAPHTIEVIAQSEWPHPYTRETAAFPAPWLRQHKFWPSVSRID
                     NVYGDRNLVCTCPPMESYPS"
BASE COUNT          849 a          872 c          936 g          767 t
ORIGIN      
        1 ccagattccc agcgatcaac tactgcggcc ccgacggcat ctccccagac attcacggcg
       61 gttcggcagc gatccagaaa ccagtccacc accaaaatca gtgaaatccc ttcaatgggc
      121 agatccacgg ctttcaggac gatgaccatg gtgaccagac ccgcttccgg gattccagct
      181 gcgccaatgg cggagagtgt cgcggtgatc aggacgataa gcgtttcacc caatcccaac
      241 tcgatgccat aggtctgtgc gataaacatg gctgcgaccg cttcatagag ggcggtccca
      301 ttcatgttga tcgtggcgcc cagtgggacc acaaaactta ccacacgtcg ggacaccccg
      361 gcttcttcga tcagactttc catcgtcaaa gggagggtgg ccgaactcga acttgtcgag
      421 aaggcggtca tgagcggcgt ggtcatcgct ttcgcatagg aaggaaccga ttgctttccc
      481 aatattcgta atgccaaggg aaggatcaag aacccatgaa tgccgagggc gataagtacc
      541 gtagcgacat aggtgcccag gctggagagc tggggccaaa aacctgaaaa tccacctgcc
      601 tctcccaatc gccctgcaat caaggctccg attcccaccg gtgccaccca catcagtaga
      661 tggacgatgg ccatcatggc atcgtttatt ccttcgaata agcgaatgac caggatgccc
      721 ttttctccga tggtggtaag gacgccacca aaaattagcg cgaagacaat caacggtaag
      781 atttgcgttt ccgccatagc ggcaaacagg ttttttggca ctaagctggt cagaatagtt
      841 ttgaacagct caacgaggga ggttggttgg tccttcatct gtaaggaaac ttccgatggc
      901 ggagccgggg cggttccgtt gggtagttgc tctagtggca ccccgggatg aagaacaatc
      961 accaggatca atccgaccaa gacggccagg cccgtggtgg tcatgaagaa cagcacggtt
     1021 cgaatcccga gaggtcccaa ttgtcgaaca tcacccaaac tggcgattcc cacaatcatg
     1081 gaactcacta ccaatggcac gacaagagca aataacgcct gaagaaatag atcacccagg
     1141 aattggatgc ccaatccaaa ggacgggaaa agacctccca gcaggacacc tgtggaaatc
     1201 ccaagcagca tgccgatcaa caggttgtgg gggccggagg tcttcaaatg actaggcctg
     1261 gttagttata gggagagaga ctagatggag ttctcgtatg gcaagccagt gatttaggaa
     1321 ggataggatt ccatgggtgg acaggtacag accaaatttc tatctccata aacgttatcg
     1381 attctggata cggatggcca aaatttatgc tgccgtaacc agggagctgg aaatgcagcg
     1441 gtttcgcggg tataggggtg tggccattcg gattgggcga tgacctcaat agtatgaggc
     1501 gcatgtttta agggattatt gtcgcgaggg aggcgtccct cctcaatctc ggcaatttcc
     1561 tggcggatca ggatgagtgc gtcgcaatac cggtctaatt ccaccttgga ttcactttcg
     1621 gtcggctcga tcatcagggt tccggggacc ggccacgaaa tcgtgggagc atggaatcca
     1681 aagtccatga gccgtttggc cacatcttcg acttctacct ctgcggattt tttgaaaggt
     1741 cgcaggtcta agataaattc gtgagccgcc atgccgtttt tcccggtaaa aagcacagga
     1801 taatatttaa tcaatcggct ggccatatag ttggcattta agatggccac ttgcgtggct
     1861 tttgtcagtc cctcactgcc catgagtttg atgaagaccc acgagatagg aagaatattt
     1921 gggctgccat atggagcagc ggcgatgggc ccgatgcctt ttgatcctcc cacttgcccc
     1981 aagggatgac ctggtagaaa gggggccaag tgaggtgcga cggcaatggg gcccatccct
     2041 ggacctcctc caccgtgtgg gatgcaaaaa gttttatgca gattgagatg gcacacatcg
     2101 gcgccaatcg cccccgggcg gaccaggccg accatggcgt tcatattagc gccatccaaa
     2161 tacacctgac cttcatgagc atggacaatt tcacagacct tgcgaatgct ttcttcaaac
     2221 acgccatggg tcgatgggta tgtcaccatt aaacaggcca aggtgtcatg gtattgggca
     2281 gactttgctt ccaaatcatg aaggtcgatg tttccatggt tatcacaggc gacaggaacg
     2341 accttcaatc cggcgataac ggcactcgca gggttggtcc cgtgcgccga taccggaatg
     2401 agacagatat tccgttgttc ttgaccccgt tcctcttgat aggcccgaat gaccatcaat
     2461 ccggcatatt ccccttgaga accggcgttg ggttgaagtg aaacagcctc aaacccggtg
     2521 atctcggcga gccatgcttc caattgctga aacagcgact gatacccttg cgcctggttg
     2581 ctgggggcaa aagggtgtag ccgactgaat tccggccatg tcacaggaac catctccgcc
     2641 gtcgcattga gtttcatcgt gcatgatccc aatggaatca tggaatggac gagtgagagg
     2701 tctttggatt gtagtagata catatatctg agcaattcgt gctcggaatg ataatcatga
     2761 aaaacaggat gggtgagaaa gggagactgt cgcttaaagg tcgttgggat ggatgaaccg
     2821 agctgatccg ccagatcgga taaggagaag ggaagtgact ccttgtcggt aaagagggtc
     2881 agtagctgac tcacatctgt gatggtggtc gcttcatcca acgcgatgcc aaaggacccg
     2941 tcggcatatt gccgaagatt gatgaatttc tgctgaccat ttttgattat ttttgcctgg
     3001 ctcaggtgag ggcaccgaac ccgtagggta tcaaagaaat cattggagtg gagttgacat
     3061 cccaagcgac gaagaccttc ggctgtgagc gtagtgaggt gatgaacttt gttagcaatg
     3121 ctctttaatc cttccgggcc atggtagacc gcatacatgc cggcaatatt ggccagcagc
     3181 acctgagcgg tacaaatatt actcgtcgct cgatcgcgcc ggatatgttg ttctcgggtt
     3241 tggagggcca ggcgatacgc cttttgaccg tgggcgtcct tggagactcc caccatccgg
     3301 cctgggatgt gtcgtttaaa ggcctctttt gttgccagaa atgctgcgtg gggaccgcca
     3361 tagccaagcg gaactccaaa tcgttgcgac gaccccaccg caatatccgc tccccattca
     3421 cccg
//
LOCUS       DAORAQ010000266         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3882_length_3408_cov_63.700268, whole genome shotgun sequence.
ACCESSION   DAORAQ010000266 DAORAQ010000000
VERSION     DAORAQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_3408_cov_63.700268"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKM72_13905"
     CDS             complement(<1..319)
                     /locus_tag="PKM72_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNP61936.1"
                     /translation="MTPLSLHIPLSTYRLQFNSSFTFQDASRILPYLSQLGITDCYAS
                     PYLKAAPGSTHGYDVVDPTELNPEIGTETEYQAFIQALHQHGMGQILDVVPNHMGIAG
                     STNL"
     gene            complement(330..1622)
                     /gene="glgC"
                     /locus_tag="PKM72_13910"
     CDS             complement(330..1622)
                     /gene="glgC"
                     /locus_tag="PKM72_13910"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNP61937.1"
                     /translation="MKSSSGSRRKKITERTPQKILAMIMAGGKGERLMPLTEQRSKPS
                     VPFAGTYRIVDFVLSNFINSGIQSMYVLVQYRSQSLIEHLRRAWRFGGTNRQNFITVV
                     PPQMKGRGKWYEGTADAVYQNINLIQDFAPGLVAVFGADHIYRMDVRQMIQFHLDQEA
                     DVTVASLPVPIQAAKGFGIVEVNANHQIIGFEEKPASPKPMPENPEMAFSSMGNYIFN
                     ADILVKILEQDASQAGTHDFGRNIIPSLIKSHRVLAYDFMDNVVPGIHPYEEWGYWRD
                     VGTLEAYWQANMDILGESPILDLRNVKWPILTDASVEPVASLVRSHMDDAMVGQGSLV
                     VDSTVKRSIIGRNVRIGEGCTIEESIILDGTLIGSNCHLHRCIIDRFNIISSGTTLGD
                     KHGRDGRRSTAGKLGLTLFPRGQSYGGRAIHSSPSSLT"
     gene            complement(1629..1910)
                     /locus_tag="PKM72_13915"
     CDS             complement(1629..1910)
                     /locus_tag="PKM72_13915"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNP61938.1"
                     /translation="MYSALLSLLLLLFFAVAFSIQNSEPIILNFFAWTFHGSRAVMLL
                     SALGLGVSLTLLASLPSYIKKSRLIAKQQKTIAALERSVAELTNPPSNT"
     gene            1967..>3408
                     /gene="glgB"
                     /locus_tag="PKM72_13920"
     CDS             1967..>3408
                     /gene="glgB"
                     /locus_tag="PKM72_13920"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HNP61939.1"
                     /translation="MPILSNRFQQLVSANEWNPFSILGPHQDDSRDSSDICIRAFLPE
                     ASEVVVLPGINESTPIPMKLVHPDGVFEARWNGHPLGTDYQFRIVDRQGKASQRHDPY
                     AFPPLISDFDLHLFGEGKLYKAYEQLGAQLCIHQGIQGVNFAVWAPNAKRVSVVGDFN
                     EWDGRRHPMRSRGGGGIWELFIPDMNDGAVYKFEILPQNGAGPFLKADPYASSAELRP
                     KTASVVRDLSGYEWQDGQWMTARQTCNPLTQPWSIYEVHLGSWKRVPEEGSRWLTYSE
                     LAQNLIPYVKEMGFTHIELMPVTEHPFDGSWGYQATGYFAPTSRFGTPVDFMAFVDAC
                     HQAGIGVLMDWAPAHFPEDPHGLAWFDGTNLYDHSDPRLGFHPEWNSRIFNYGRTEVK
                     NFLINSALSWFDRYHIDGLRVDAVASMLYLDYARKSGEWIPNKFGGRENLEAVEFLKE
                     LNTVAHQEHPGIVMIAEESTAWPGVSRPTYV"
BASE COUNT          839 a          775 c          919 g          875 t
ORIGIN      
        1 aaagattcgt cgatccggct attcccatat gatttggcac gacgtccaga atctgtccca
       61 tgccatgctg gtgcagtgct tggataaatg cctgatactc ggtttccgtt ccaatctctg
      121 gatttaattc cgtgggatca accacgtcgt atccatgagt gctccctgga gcggctttca
      181 gatagggcga ggcgtagcag tcggtgatac caagttgaga cagataggga agaatgcgag
      241 aggcgtcctg gaatgtgaac gacgagttaa attggaggcg ataggtcgag agcggaatgt
      301 gcagagacag aggagtcatg ctccctcctt tatgttaaag aggagggtga ggagtgaatg
      361 gcccgtccgc catacgattg cccacgaggg aaaagggtga ggccgagctt cccggctgtg
      421 gatcgtcgac cgtctcgtcc gtgtttgtca cctagggtgg ttcctgacga gataatatta
      481 aatcgatcaa tgatacaacg atggagatgg caattggagc caatgagtgt cccatcaaga
      541 ataatcgatt cttcaatggt acagccttct ccaattctca cattacgtcc gatgatggaa
      601 cgtttcaccg tactgtctac gacgaggctg ccttggccga ccatggcatc atccatgtgg
      661 gatcggacta gactggcaac gggttcaacg gacgcatcgg tcagaatagg ccatttgaca
      721 tttcgtaagt caaggatcgg cgactctcct aagatgtcca tgttggcttg ccaataggcc
      781 tccagcgttc cgacatcgcg ccagtacccc cattcttcat aaggatgaat gccgggaacc
      841 acattgtcca tgaagtcata agctaaaacc cggtgagatt tgatgaggga tggaatgatg
      901 ttcctgccaa aatcgtgagt gccggcttga ctggcgtctt gttccagaat tttcacaaga
      961 atgtcggcgt taaaaatata attccccatc gaactaaagg ccatctcagg attttcaggc
     1021 atgggtttcg gcgaggctgg cttttcctca aacccgatga tttgatggtt ggcattgact
     1081 tcgacaattc caaacccttt tgctgcctga atcggaacgg gaagcgacgc gacggtcaca
     1141 tccgcttcct ggtcaagatg aaattggatc atttgccgga catccatccg gtaaatatga
     1201 tcggctccga agacggccac caatcctggc gcgaagtcct gaatgaggtt aatattctga
     1261 tagacggcat ccgctgtccc ttcataccat ttgcctcgtc ccttcatttg tggagggacg
     1321 accgtaataa agttttgacg gttggtgcca ccaaaacgcc aggcccttct gagatgttca
     1381 attaaggatt gcgaacggta ttgcaccaaa acatacatgg attggattcc ggaattaatg
     1441 aaattgctca gaacaaaatc gacaatgcga taggtgccgg caaagggcac ggatggctta
     1501 cttctttgtt cagttaacgg cataagccgt tcaccttttc cgccagccat gatcatagcc
     1561 aggatctttt gtggcgtccg ttctgtgatt tttttgcgac gggatccaga agagcttttc
     1621 atatgggttt aggtgttgga tggagggttg gttaactcgg cgacggatcg ttccagtgcc
     1681 gcgatcgttt tttgttgttt ggcaatcaac cggctttttt taatgtagga gggcaatgaa
     1741 gccagtagag tcaggctaac acctaacccc aaagccgaga gtaacatgac cgcccgcgac
     1801 ccgtggaagg tccaggcaaa aaaattcaga atgatgggtt cggagttttg tatagaaaag
     1861 gcaacagcaa aaaatagtag aagcagcaac gacaacagtg cagaatacat aatccctttt
     1921 ggtatgttag gatattgtta attaacatag aaaggattca ttggcaatgc caatactctc
     1981 aaatcgtttt caacagctgg tttctgccaa cgaatggaat ccgttttcga tattaggtcc
     2041 tcatcaggac gattcgaggg attcttctga tatctgcatc agggcatttc tgcctgaggc
     2101 ttccgaggtg gtggtgcttc ctgggataaa tgagagcacc cctatcccca tgaagctcgt
     2161 ccaccctgac ggggtttttg aagcaaggtg gaacggtcat ccacttggca cggattatca
     2221 attccgcatc gtcgatcgtc aggggaaagc cagccaacgt catgacccct atgcgtttcc
     2281 cccgctcatc tcagattttg acttacattt gttcggtgag ggcaaactct ataaagctta
     2341 tgaacagctt ggggcgcaac tctgcatcca tcagggaatt caaggggtga attttgctgt
     2401 gtgggctcca aatgctaagc gagtgagcgt ggtcggagac ttcaatgaat gggatggccg
     2461 tcggcatccg atgaggagtc gtggaggggg gggaatctgg gagttattta ttccggatat
     2521 gaatgatggt gcggtatata agttcgaaat tttaccgcaa aatggtgctg ggccgttttt
     2581 gaaggctgac ccgtatgcct cttccgctga actccgacca aaaacggcct ctgttgtgcg
     2641 tgatctttca ggatatgaat ggcaggacgg gcaatggatg accgccaggc aaacctgtaa
     2701 tcccctcact cagccttggt ccatctatga ggtgcatttg ggatcatgga aacgtgttcc
     2761 agaggaagga tcccgatggt tgacctactc cgaattagct caaaacctca ttccgtatgt
     2821 gaaggaaatg gggtttaccc acattgagtt gatgccggtg accgaacatc cgtttgacgg
     2881 gtcctggggg tatcaagcca ccgggtactt tgcaccaaca agccggtttg gcactcctgt
     2941 ggactttatg gcatttgtgg atgcctgtca tcaggcgggt attggggtgc tgatggattg
     3001 ggctccggcg catttccccg aagatcccca cgggttggcc tggtttgatg ggacaaattt
     3061 atatgaccat tccgacccga ggctggggtt tcatccagaa tggaatagcc ggatctttaa
     3121 ttacggccgg actgaagtca aaaacttttt gatcaatagc gcactgagtt ggttcgaccg
     3181 gtaccacatc gatggtcttc gcgtggatgc tgttgcgtcg atgctgtatc tcgattatgc
     3241 gcgaaaatcc ggtgaatgga ttcccaataa attcggaggc agagaaaatc tggaagcggt
     3301 ggaatttttg aaagagctga acaccgtggc tcatcaggaa catccgggta ttgtgatgat
     3361 tgcggaagaa tccaccgcat ggcctggggt ctccaggccg acctatgt
//
LOCUS       DAORAQ010000267         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3938_length_3360_cov_68.523449, whole genome shotgun sequence.
ACCESSION   DAORAQ010000267 DAORAQ010000000
VERSION     DAORAQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3938_length_3360_cov_68.523449"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKM72_13925"
     CDS             <1..370
                     /locus_tag="PKM72_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61940.1"
                     /translation="VLRPGKLCMKIEAVFFRALFGVIVFTELGLADVFEVKEAVEGVI
                     CRKLATDYATDPTTLTIQAIAQLQICLAQTLKNSASSSALPLPRNFDIQPSRSSTSIG
                     DTTLPTPPTPPTPPKSLKIR"
     gene            complement(652..1842)
                     /locus_tag="PKM72_13930"
     CDS             complement(652..1842)
                     /locus_tag="PKM72_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNP61941.1"
                     /translation="MQNIRHDHILEGILITLRGSLAACPEVAGSEVMTSTLIANFLHS
                     YAPDKILTHLGGYGIAAVYEGQHPGPTVMIRCELDGLPVEEADKNQWLVPGANIAHRC
                     GHDGHMAIVAGLAPLLHRTPPSCGRVVLLFQPAEETGAGARAIIDDPNFEKVRPDYAI
                     TMHNLPGFPSGHVVYRRGTFASASVGMRVRLLGQASHAAEPEQARSPANAVGQLLAEL
                     PRLSRITDNPYRMLTLTHAQLGLMSFGLTPGEATVCATLRAASAASLQTLQREAEQCV
                     RDCAAAELLFAEVEWVQDFPATVNDDALVDTLERVCLDAGIVAIEIPKPFRWSEDFGH
                     FAEICPILHFGLGIGEHAPGLHQPDYEFCDETILIAVSGFYEMVKSLLIGYGNRGENA
                     ATGT"
     gene            complement(1981..2997)
                     /locus_tag="PKM72_13935"
     CDS             complement(1981..2997)
                     /locus_tag="PKM72_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNP61942.1"
                     /translation="MRIFITGGAGFLGSHLCETLILQGHSVICLDNLLTGRTENIGAL
                     MGHPKFSFIHYNVCDYLHVDGPVDAVMHFASPASPQDYLEFPIATMKVGALGTHKALG
                     LAKAKQARFLLASTSEVYGDPLVNPQPETYWGNVNPISPRGVYDEAKRFAEALTTAYH
                     RFHGLDTRIVRIFNTYGPRMRPKDGRVVSNFIVQALQGHPLTVYGDGSQTRSFCYVDD
                     LVAGIVALLQAPADELRKNQEFQDSVRFGLTQANLGSIHDPVNIGNPHELTVMDIAET
                     VIKLTNSSSTITQHPLPIDDPKVRRPNIEKAKTLLKWEPRITLEQGLDKAIDYFRSHF
                     DLAK"
     gene            3093..>3360
                     /locus_tag="PKM72_13940"
     CDS             3093..>3360
                     /locus_tag="PKM72_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61943.1"
                     /translation="MPFSNHPPFSFPLTRRALFKLGGLASIGFGIPGCDMGSMFAVPP
                     RETTYFTSNDKFYTVNFMDASYNFTRDLDVEQWKMVVKGAVERPV"
BASE COUNT          786 a          846 c          866 g          862 t
ORIGIN      
        1 tgtccttcga ccaggaaaat tatgcatgaa aatagaggcg gtgttcttta gagcgttgtt
       61 tggggttata gtctttacgg agttgggttt ggccgatgtg ttcgaagtca aggaagctgt
      121 ggaaggggtc atttgccgaa agctggccac ggattatgca accgatccta ccacccttac
      181 catacaagcc atcgcgcaac tacaaatttg tcttgctcaa acattgaaaa attcggcctc
      241 gtcttcggca ttaccattac ctcgaaattt tgacattcaa ccttctcgct cttcgacaag
      301 tattggcgac accacgcttc caactccacc aactcctcca acacctccaa agtccctgaa
      361 aattcgatga ggattgtctt gtcgcccttc tcttaaagta ctgtccaatt aagcaagata
      421 tattaagaag gctaatggaa atggtcgtgc agccttctcc cttttgctgt tgtgtcgtac
      481 tcgcccgaaa tcaagccacc cgtcaggcca attcctgtct atattgacga tggttccggc
      541 cgaacccttg aacggttcgt agatccctcc accggtttct ggtgttgatt agagttgtct
      601 ctggaattac tgtcttagta gaagtaggtt tggcgatctt ctcgaacctg atcaggtacc
      661 cgtcgcggca ttctctcctc gatttccata tccgattaaa agcgatttga ccatttcata
      721 aaaaccactc acggcaatca ggatcgtctc gtcgcagaac tcataatccg gctgatggag
      781 gcccggtgca tgctcaccaa tgccgagtcc gaaatgcagg atagggcaaa tctcggcaaa
      841 atgcccaaag tcctccgacc agcggaacgg tttgggaatt tcaatggcca caattcctgc
      901 atccagacac acgcgctcca aagtatcaac caaggcatca tcgttgacgg tcgctggaaa
      961 atcctgcacc cactcgactt ccgcaaagag cagttccgct gcggcacaat cgcgaacaca
     1021 ctgttcagct tcgcgttgca aggtttgaag gctcgccgca gaagctgcac gcagcgtggc
     1081 acacacggtc gcctctcccg gtgtaagccc aaacgacatg agccccagct gggcatgagt
     1141 caacgtgagc atacgatagg gattgtccgt gattctgctc agtcggggga gttcagcgag
     1201 tagctgtccg accgcatttg caggtgaccg agcttgttca ggctcggccg cgtgggacgc
     1261 ttgaccaagg agacgaactc tcattccgac agatgcactg gcaaatgtcc cccttcgata
     1321 cacgacatgc ccgctgggaa agcctggtag attatgcata gttatggcgt agtccggccg
     1381 gaccttctca aaattcggat catcgataat cgcccgtgcg cctgcccctg tttcctctgc
     1441 gggctggaat aatagcacca ctcggccaca gcttggtggc gtgcgatgaa gcaggggtgc
     1501 caggcccgcc acgatggcca tatgtccgtc atgaccgcat cggtgggcga tgttcgctcc
     1561 aggaacgagc cattggtttt tgtctgcttc ttctacagga aggccatcca gttcacatcg
     1621 aatcatcacc gttggcccag gatgttgtcc ttcataaacc gctgcgattc cataaccacc
     1681 gagatgagtc aggattttgt ctggtgcata ggaatgcaga aagtttgcga taagtgttga
     1741 ggtcatgacc tctgatccgg ctacttccgg gcaagccgca agggaaccgc gaagggtgat
     1801 taaaatgcct tcaagtatgt ggtcatgacg aatgttctgc atgtttttat tctatggtgg
     1861 caaggtgtct ctgtccactg gcccagatca gttgcagtcg gagcattggc ggaacgcggg
     1921 cttatcgagc ccataatggg atacagattt cccggaatcg atcaacctct aatccttgat
     1981 tcactttgcg aggtcgaaat gagagcggaa atagtcgatg gctttgtcga ggccttgttc
     2041 taaagtaatc cgaggttccc acttaagcag ggttttggcc ttctcgatat tgggccggcg
     2101 cactttgggg tcgtcgatgg ggagggggtg ttgcgtaatc gtgctcgatg agttggtgag
     2161 tttgatcact gtctcggcaa tatccataac cgtgagctca tgtggattgc ctatgttaac
     2221 tgggtcatgg atagatccaa ggttagcttg agtcaatccg aaccgtaccg aatcctgaaa
     2281 ttcttgattc tttcgaagtt cgtccgccgg ggcctggagg agggcaacaa ttcctgcgac
     2341 cagatcatca acgtaacaaa agctacgggt ctgagaccca tcgccataca cggttaacgg
     2401 atggccttgt aatgcttgaa cgataaaatt ggacaccacc cgcccatcct ttgggcgcat
     2461 ccgtggacca taggtattga aaattctgac aattcgagta tcgaggccat ggaaccgatg
     2521 ataggcggtg gttaaggcct cggcaaatcg tttggcttcg tcatacactc cacggggact
     2581 aatgggattg acattgcccc aataggtttc aggctgtggg ttgaccagtg gatccccata
     2641 cacttcagag gtactggcta gaagaaatcg cgcctgtttc gccttggcta agcccaaggc
     2701 tttgtgcgtg cctaacgcac cgactttcat ggtggcgatg ggaaattcca gatagtcctg
     2761 tgggctcgcc ggtgaggcaa aatgcatgac ggcatcgact ggtccgtcga catgaaggta
     2821 atcgcagaca ttgtagtgaa taaatgagaa cttcggatgg cccatgaggg caccgatatt
     2881 ctctgttcgt ccggtaagga gattgtccag gcagataact gaatgtcctt gaaggataag
     2941 cgtttcacat aaatgacttc ctaggaatcc ggcgcctccg gttataaata tccgcatgcg
     3001 atttctcctt ctatacttct gaatttgtgt gatccaatga acgggagacc ttgtccgaag
     3061 ctgcaagagt gttagataat agggtaatgt ctatgccatt ttcaaaccat cctccttttt
     3121 cttttcctct gactcgtcgg gctctcttca agcttggggg actggcttcc attggcttcg
     3181 gaattcctgg atgtgatatg gggtcgatgt ttgcggtccc tccccgggaa accacgtatt
     3241 tcacctcgaa cgacaaattt tataccgtga atttcatgga tgcttcctat aattttacgc
     3301 gggatctgga tgtggagcaa tggaaaatgg tggtcaaagg cgccgtggaa cgccctgtgg
//
LOCUS       DAORAQ010000268         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_3976_length_3335_cov_61.474390, whole genome shotgun sequence.
ACCESSION   DAORAQ010000268 DAORAQ010000000
VERSION     DAORAQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3976_length_3335_cov_61.474390"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..460
                     /locus_tag="PKM72_13945"
     CDS             188..460
                     /locus_tag="PKM72_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61944.1"
                     /translation="MKLTMKWGGKVRNRKTFKPEPKIRWDLLGLADPSKEKIPMPIDE
                     VRRQVLMMSSPGFDQDSATRLDVVPQKISRSSTSAKPVTKTRREST"
     gene            631..819
                     /locus_tag="PKM72_13950"
     CDS             631..819
                     /locus_tag="PKM72_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61945.1"
                     /translation="MGRPTKVTQAKRNREMVKRMKQQDKEARREQRKSENEEPPRVLD
                     GEDPDLVGIRPGPQPPQY"
     gene            1588..2151
                     /locus_tag="PKM72_13955"
     CDS             1588..2151
                     /locus_tag="PKM72_13955"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNP61946.1"
                     /translation="MKLKTLIECVVLGLGCVMGGCAASPMEIPETLRNQIDPALTFSQ
                     VIQHPGSYQGKILLLGGEVLSVKRLKEGTRLEVLQLPLDEYRRPLMTRTDSQGRFLAM
                     EKTFLDPAMFPPHTLITLVGEVTESVAGKLDEMDYQFPAVVIKDLYVWKDPSLTQASN
                     SGPWSSIFGGGSTGGRVGGGIGVGIGF"
     gene            2466..3092
                     /locus_tag="PKM72_13960"
     CDS             2466..3092
                     /locus_tag="PKM72_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNP61947.1"
                     /translation="MQKEPEESTACVSSEGERRAQNMMGTGRRAAAFYRNQMESELND
                     NMQSFIARQEMVFLSTANAKGECDCSFRAGTVGFVRVLNPRMLAYPEYRGNGVMASVG
                     NILENPQIGLMFIDFLQTTVGLHVNGKAKVLTNDDIMRLPNVTAAILEDVTVAGGRHP
                     ECWTLVEVEEAFIHCSKHIPLLKKLDKPLHWGTDDESLKGSGFFKEIR"
BASE COUNT          802 a          787 c          902 g          844 t
ORIGIN      
        1 cacggtgcaa gcgatggtgg ttaccctgcc ttttccgcgc tttcattcct aggcggattg
       61 atttttacaa ttgattcttg agggtgtgaa accttcatta accccttcgc ttcctgcccg
      121 gttcgacagg acggttcgtg ttctttgtca tgattgggat ggtttttttg ccagaaggag
      181 gcttcttatg aagctgacaa tgaagtgggg tggtaaggtc aggaatagaa aaacctttaa
      241 accggagccc aagatccgtt gggatctcct tggcctcgct gatccatcta aagaaaaaat
      301 ccccatgccg atcgatgagg ttcgccggca agttctgatg atgtcgagcc ctgggtttga
      361 tcaggatagc gctactcgcc ttgatgtggt gcctcaaaaa atttcccgtt cttccacttc
      421 tgcaaagccg gtgaccaaga cgcgcaggga gtccacgtag gacggctatg ccgcgtaggt
      481 ggacaagaga cgggtacatg ggagcatggc aggttcggtg gctcgacaag tgggtgtgat
      541 ggtctctcaa acaggcatag acttaaagag tcctaacact caagtgtgga agccggaggc
      601 gtgataaccc atatcaaagg aggaagataa atgggaagac ccacaaaagt aacccaggcc
      661 aaacgaaatc gtgaaatggt caaaaggatg aagcaacagg ataaggaagc ccgacgtgaa
      721 caacgcaagt ctgaaaatga agaacctcct agagtactcg atggggaaga tcccgactta
      781 gttgggatac ggccgggccc gcaaccacct caatactagt gagacctgtc ccttctcact
      841 aatcgttcga cacaaatacc ggtattcttc cggggatctt ttccccaact caagtttttc
      901 tgccaaggtt ggctctagta tccgaaaaat ctgcctcact ctgcttttct gacagcccgg
      961 tagtacccgt agggggagaa atggcatgtc aggtcgtgga tattactgta gttcacctta
     1021 ctgtgggtga ccattcccga acaaccctgt tgaataggcg tgcgtctaca tggaatctaa
     1081 agaaaggcca gcctacttcc aggttgttat tttataacct gtttgtctgc atcggttggc
     1141 cggttcctgt gagagggtgg cacccttccc attgggagtt catatagaga gtatcgggcc
     1201 acttcatgct ccaaggcaga ttccctggag gtccggtgat gcgggttaag ttctcccaat
     1261 ttttggtcgt cacagtctct tgttatgtga gacgcatcgc tacttccgtt ggttgttccg
     1321 aggccataat tagcattacg atttgtctga gttgagtctt cctgttgagc ttggtcaaat
     1381 cggaccaggg tgaaaatccc gttgcgcctc tttgcatcac cgcgccagcc aggcgaagtt
     1441 aagatgggtc acggagaatt ctaaatgttg gtatcatctg tgagggcttt aactaatttc
     1501 atgccgtttt tcaggcccgg ttggagtgat tattttccga ggggatacca tccgccaatc
     1561 atccatagga tataatagaa taggcttatg aaactgaaaa ctctcattga gtgtgtggtt
     1621 ctcggtctgg ggtgtgtaat gggaggatgc gctgcctcgc ccatggaaat accggaaacg
     1681 cttcggaatc agattgatcc tgctctcacg ttttcgcaag tgattcaaca tcccgggtct
     1741 tatcaaggaa agatactcct gcttggaggg gaagtccttt cggtcaagcg ccttaaggag
     1801 gggacccggc ttgaggtgtt gcaattaccc ttggatgagt accgacgtcc cctaatgacc
     1861 cgcaccgact cacaaggccg gttcctcgcc atggagaaaa ctttccttga tcccgccatg
     1921 tttccgccgc atactctgat cacccttgtg ggagaagtga cagagagtgt cgccggcaag
     1981 cttgatgaga tggactatca gttccccgcc gtagtgatta aagacctcta tgtgtggaaa
     2041 gacccgtctc tgactcaagc aagcaattcc ggtccctggt ccagcatctt tggtggtggg
     2101 agcaccggag gccgggtcgg tggggggatc ggagtgggca ttggctttta gtgtcaattt
     2161 gttcttcggc cgtagaattt tcccctcatg accacctccg gctatacgtt gagggatgag
     2221 cctcgatacg gcgaggcatg gactgtattc ctcacacccc tccgtcaaca cctttagcgg
     2281 ttccatcaaa taattgatcc tgatcctcct tcctccattt tctgaaaggt gatggggatg
     2341 tggttctttc cttcagcaaa cctgtgcgtc taagataacg ggtgccctcc cactagagaa
     2401 tttcattaat ttacgaaggc aaggccggcg caggtttggc cttgaagata aactgaaggt
     2461 gtaagatgca aaaagagccg gaagaatcga cagcgtgcgt gagttcagaa ggagagcgtc
     2521 gagcccaaaa tatgatgggg acaggccggc gggccgcggc tttttatcgg aaccagatgg
     2581 aaagcgaatt aaacgacaat atgcagtcgt ttattgccag acaggagatg gtttttcttt
     2641 cgaccgccaa tgccaagggc gaatgtgatt gttcgtttag ggcggggacc gtcggattcg
     2701 ttcgagtgct caatcccagg atgttggcct atcccgagta tcgcggaaac ggggtcatgg
     2761 cgagtgtcgg caatatactt gagaacccac aaatcggcct gatgttcatc gattttttgc
     2821 aaaccacggt cggtctgcat gtcaatggaa aggcaaaagt cctcaccaac gatgatatca
     2881 tgcgtcttcc caacgtcacg gcggcgattc tcgaggacgt gaccgtggcc ggaggacgtc
     2941 atcccgaatg ctggacgttg gtcgaggtgg aggaagcctt tatccactgc tccaagcata
     3001 tcccactgct caaaaaatta gacaaacctc ttcattgggg taccgacgat gaatccctca
     3061 aaggcagcgg gtttttcaaa gagatccgct gagaacccat attgaatatt ggaccaagat
     3121 cacgtgacga tatgcacaaa gacggttgta ggagaaggat gtgttgtact caccacatca
     3181 accatgtctg attgaaggga tcttatgaaa tcacccgtac gggagtggat cattggatgc
     3241 gtgatcatat tgttctgtcc acttccggtg ttggccacgg ttggctggga aacgggggac
     3301 atcgttctgg atttcgggct gaatctggaa tccgg
//
LOCUS       DAORAQ010000269         3298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4022_length_3298_cov_67.101758, whole genome shotgun sequence.
ACCESSION   DAORAQ010000269 DAORAQ010000000
VERSION     DAORAQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_3298_cov_67.101758"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKM72_13965"
     CDS             complement(<1..363)
                     /locus_tag="PKM72_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61948.1"
                     /translation="MTDQEHIRIFDSQGEAYKQAFQIFLNHTDQKRNAKRWLRQVIND
                     LPGRQVFVDAGAGNGELTRAFTDAFDRTIAIEPNVFLMNQLQQAIPTAEAIGLPILSA
                     QPSTPGDLVLSSHTFYYIP"
     gene            738..1406
                     /locus_tag="PKM72_13970"
     CDS             738..1406
                     /locus_tag="PKM72_13970"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNP61949.1"
                     /translation="MCGRFTRKENFQHLAKQLGLQVVPPMGPRYNIAPSQFIACVRTN
                     LETRQRECTELKWGLVPSWAKDPGIGNKLINARGETVAEKPAFRKAFKQQRCLVLADG
                     FYEWKREGKTKQPFYVRFKDHRLFAFAGLWERWKQNEEDTLETCTLITTTPNAVMEPI
                     HHRMPVILSSKDFADWLDPTLQAVERVNTLLRPFPPEEMEAYSVSQLVNNPRNDRPEC
                     INPI"
     gene            1808..2296
                     /locus_tag="PKM72_13975"
     CDS             1808..2296
                     /locus_tag="PKM72_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61950.1"
                     /translation="MKMHTSKSVWSLTMFLVLSLGMWGTSTSAQESESSKDSAAFSDV
                     GPSLQELGEAQRDIDRYEEAQDNFNENVDQFMNDRMRYNEARAKFDRSRSIYEQEEHR
                     FNKTYEKFSRDRDRYLEARDKFVGARERFQDARSNFFGSSINASSRSNDGRMKDNPAD
                     KR"
     gene            2414..2725
                     /locus_tag="PKM72_13980"
     CDS             2414..2725
                     /locus_tag="PKM72_13980"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNP61951.1"
                     /translation="MKYSIKMKMVACLFFVFLAVGCAGGPQKESFGEHIDDSVITTKV
                     KTALLSDPEVSGTDINVTTFKGRVQLNGLVNDAQQIDQAVQLARRVGGVQAVENNLSI
                     K"
     gene            2827..3096
                     /locus_tag="PKM72_13985"
     CDS             2827..3096
                     /locus_tag="PKM72_13985"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNP61952.1"
                     /translation="MMMMSMGCASNPLHESTGEYLDDSVITTKVKTALLRCPLVSGTD
                     VHVETFKNRVQLSGFVDEMRQIDHAILLSREVEGVGLIENRMSVK"
BASE COUNT          903 a          703 c          820 g          872 t
ORIGIN      
        1 agggatgtaa taaaatgtat gggaggagag caccaaatcg ccaggggtgg atggctgggc
       61 ggacagtatt ggcagaccta tggcttctgc agttggaatg gcctgttgta gttgattcat
      121 cagaaagacg tttggttcga tggcaatggt gcggtcgaag gcgtcggtga aggcgcgggt
      181 gagttcgccg ttcccggccc cggcatcgac gaacacctgt cgccccggca gatcatttat
      241 gacttgccgc agccaacgct ttgcgttccg tttttggtcg gtatgattta agaaaatttg
      301 aaaggcctgt ttgtaggcct ccccctggga atcaaagatg cgaatgtgtt cctggtcggt
      361 catcgttggg gcctccggtg atctttcgca atgaagaggt atttcctgca gtttcctgga
      421 aaatgaaaac atggtcctgt atttttttgt tccacatccg tgaataacag atagtttgtg
      481 atctgaaaaa ttacgctcaa aaaaatctca acaaccgcga tgaaaagaat gatatcagga
      541 accagggaat ttaacatcca tgattggtta aagaaaaaac gaggttcagt catcccacat
      601 acatatggtc gagttgaata accgtgaaag agaccctcga ctcattttcg ttaatggcgg
      661 cctttatgaa ttttcttcca tttattccca tttttttgta gggttgggtt caaccggaaa
      721 cttcatagga cccaattatg tgcggtcgct tcacacgcaa agagaatttt cagcatttgg
      781 cgaaacaatt ggggctacag gtcgtccccc caatgggtcc tcgctataat atcgctccct
      841 cgcagttcat agcctgtgta cgcaccaatc ttgagactcg acaacgagaa tgtacggaat
      901 tgaaatgggg actggtgccc tcctgggcca aggatcccgg catcggaaat aagttgatta
      961 atgctcgcgg agagactgtc gcggagaagc cagccttccg caaagcgttt aaacagcaac
     1021 gctgtctggt cttggccgac ggcttttatg aatggaaacg ggaagggaaa acaaagcaac
     1081 ctttttacgt tcgcttcaaa gatcaccggc tctttgcgtt cgcaggcttg tgggaacggt
     1141 ggaagcaaaa tgaggaggat actttggaaa cctgcacact tattacgact actcccaatg
     1201 cggtgatgga acctatccat catcgtatgc cggttatatt atcttccaag gactttgctg
     1261 actggcttga tccaactttg caagctgttg agcgagtcaa tacgttgctc agaccatttc
     1321 ctcccgaaga gatggaagcc tactccgtca gtcaactcgt gaataatccc agaaatgatc
     1381 gcccggaatg tataaacccc atttagtcgc cccactggtt taatcccgat ctttctcatt
     1441 cagatgaaag aaaggaaatg accaatctga ttcttgaatg aagtcttttt ttgtatgccc
     1501 ccatgcggta aaaatccact aacgccaatc tgcatccact gtctatccca aacttttttc
     1561 ctccgtttta aaaaaaccct cgggccatgg tgatgacacg tcacgattcc ctccctctta
     1621 cgaatccctc ccatttcgtg tagacgggga atagacgatg ccgttccatt tgtagcatct
     1681 tttttaaaga ccgggaaagc aagggtttct gtcagttggt ctgaacattc ccccgtggta
     1741 ctcatagggt atacacagtg tcatcccccg atcggaatta accacaaatg ccaaggatgg
     1801 aggttttatg aaaatgcata cgtcgaagtc agtttggtcg ctcaccatgt ttctggtgct
     1861 ttcattgggt atgtggggga cgtctacctc tgctcaagaa tctgaatcat cgaaagattc
     1921 ggcagcattc tcggacgtgg ggccttccct gcaggaacta ggggaagccc aacgggacat
     1981 tgatcggtat gaagaagcac aggacaattt caatgaaaat gttgatcaat ttatgaatga
     2041 ccggatgcgg tacaacgaag ccagggcaaa atttgatcga tcgcgatcca tatatgaaca
     2101 ggaagagcat cggttcaata aaacctatga aaaattcagc cgagaccgcg atcggtattt
     2161 agaggcacgg gacaaatttg tgggtgcccg agaaagattt caagatgccc gaagtaattt
     2221 ctttggtagt tcgattaacg catccagccg gtctaatgac ggacgcatga aggataaccc
     2281 ggccgataag cggtaacctg tttatcgagg aacagggaag gaacgaagga gtactgatct
     2341 tacaaacttt caacgagaca ttctgatgaa ttgtttcatt cacgaatgaa tttaaaaaaa
     2401 ggagtaaagg tttatgaaat actcaatcaa gatgaaaatg gttgcttgtc ttttctttgt
     2461 atttttggcg gtgggatgcg cgggtggtcc tcaaaaggaa agtttcgggg aacatattga
     2521 tgacagcgtc atcacgacca aagtcaaaac cgctcttctc agtgatcccg aagtgtctgg
     2581 cacggatatc aatgtgacga ccttcaaagg tcgggtacaa ttaaacggtc ttgtgaatga
     2641 cgctcagcaa attgatcagg ctgtacaatt ggcacggcgg gtcggtgggg tccaggcagt
     2701 cgaaaacaat ctgtccatca agtaaatgaa ccatgtcacg atgagttcaa cttgaatatc
     2761 aaaggttttt actacgagga aacgtagaaa atgcaaaatg gcctggttat cggtgggatc
     2821 actttcatga tgatgatgtc catgggatgt gcaagcaatc ctcttcatga aagtacggga
     2881 gaataccttg atgatagcgt cattaccacg aaagtgaaga ccgctcttct ccgatgtccc
     2941 ctggtgtcgg gtaccgatgt tcatgtcgaa acatttaaaa atcgtgttca actcagcggg
     3001 ttcgtggatg aaatgaggca aattgaccat gcgatccttt tatcgagaga ggtggaggga
     3061 gtgggcctga ttgaaaatcg aatgagtgtt aaatgaagaa taccttttcg gttgaaatgg
     3121 attaaaaata tgcagtgagt gaaggtggct caggaagtaa aggggacctt tggtattgtt
     3181 cctcttatgg aattttcctt ggtcatacat gagaggaaaa aattgctcga tggaccttgt
     3241 tcatctttgc gtgcccttcg ttttaaatca agggatgggg gccttccccc atccttag
//
LOCUS       DAORAQ010000270         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4027_length_3296_cov_64.837396, whole genome shotgun sequence.
ACCESSION   DAORAQ010000270 DAORAQ010000000
VERSION     DAORAQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_3296_cov_64.837396"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..2303
                     /locus_tag="PKM72_13990"
     CDS             120..2303
                     /locus_tag="PKM72_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNP61953.1"
                     /translation="MEQPLPKTPRLSLSAKILLGMGLGIIAGIFFGELTQPIGIVGQA
                     FIQLLQMAILPFLMASLMSGLGSLTYEDAISMGKKAGGILALLWVIALTVVAVLPLTF
                     PQWPSAMFFSPSLIEETQPLDFLQLYIPSNPFFSLANSIVPAVVLFSIVVGIALIGIE
                     KKQSMLDLLSVIMKVMTRVTEFVMKLAPVGVFAIAASAAGTLNFEELGRLQIFLISQM
                     VAAVFLTFWVLPALVASLTPLPYMKILRSVRGALVTAFATGNSMIILPLLTEGGRKLL
                     EEAELANEETTSAIEVIVPTSYTFPSSGLLLSLAFIPFAGWFAGSTLSPAHYPGFLVS
                     GLASFFGGSMIGIPFLLDLFRIPADMFQLFVTVDVFTGRLGTMTAAMHMWVLGLLGSC
                     ALAGKLRVRWRKLAGYLTVCFLTGIVLLGGTRLFFTYAIESEYTKYQSFVEMALRFEP
                     AETGIRELTPDEKTVAPSENTDMEAIRQRGVLRVGFFKNSLPFVFQNETSELVGFDIE
                     MAHLLANDLGVNVEFVRLPDRDKIVEYLNDNVCDIVMSGSALRPEMTESVIFSDGYMD
                     ATIAFLVRDHDHDVFSSWEKIRSAGDLKIGVSSHSQYYQAFARSLLPQATLIPVNSPR
                     EFFKEHGKDFDALMYIAEAGSAWTLIYPAYSVAVPLPNPIKIPLVYPMPKSHRNFADF
                     VNAWLRLKKRDGTLDAVFEHWILGRGAKKKTLRWSVIRDVLHWVN"
     gene            complement(2738..>3296)
                     /locus_tag="PKM72_13995"
     CDS             complement(2738..>3296)
                     /locus_tag="PKM72_13995"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HNP61954.1"
                     /translation="KAIHRIARTLVFMVLIVPGIGLAPAQAMTAAEINSEVQEALAKL
                     YAHSKTAKDFGAVAKGILVFPDVYKAGFLVGASFGDGALLENGKTAGYYRTVEASYGL
                     QAGVQSFGYVLFFMQDEALDYLDKSDGWEIGVGPSLVVVDKGFAGSMTTTTAKDDIYA
                     FIFDQKGLMGGIGIKGSKITRIHPD"
BASE COUNT          757 a          824 c          833 g          882 t
ORIGIN      
        1 aaaggtctac atttgacttg tcctcgattg ggggaagctt acaatctgta cttgagtgaa
       61 agaattaggg taaaagaggt cgcatggaaa gatatcggga ttcctcttag ttcataacta
      121 tggaacagcc tcttcccaaa actccccgcc tgagcctttc tgccaaaatt cttcttggaa
      181 tgggtctagg cattatcgcc ggaatttttt tcggggaact tacccaaccg atcggtattg
      241 tcggccaggc ttttattcaa ttattgcaga tggctattct gccgttcttg atggcctctc
      301 tgatgagcgg actagggagc ctgacgtatg aagatgctat ctccatgggc aagaaggccg
      361 gagggatttt agcgctctta tgggtaatcg cactaaccgt ggtggcggtt cttcccctga
      421 cctttcctca gtggccatcg gcaatgtttt tcagcccctc cctgatcgaa gagactcaac
      481 cgctcgattt ccttcaactg tatatccctt ccaatccatt tttttctctt gccaacagta
      541 tcgttccggc cgtggtgctg tttagcatcg tggtaggcat tgcgttgatc ggcatcgaaa
      601 aaaaacagtc catgcttgat ctgctatcgg tcatcatgaa agtcatgacc agagtgacgg
      661 aatttgtgat gaaattggcc cccgtcggtg tgttcgccat cgccgcgagc gcggcgggaa
      721 ccttgaactt cgaggaattg ggacgtttac aaatattcct catttcccag atggttgcgg
      781 cggtcttcct cacgttctgg gtcctgccgg ctctggtagc tagcctcacc ccgttgcctt
      841 acatgaaaat tcttcggtcg gtgcgtggcg cactcgtgac agcctttgcg acgggtaatt
      901 ccatgatcat tcttcccctg ctgactgagg gaggccggaa attgctggaa gaagccgaat
      961 tggccaatga agaaacgacc tccgcaattg aagtcattgt gccgacttcg tatacgtttc
     1021 cttcctccgg cttgttactc agcctggcgt tcattccgtt tgccggatgg tttgccgggt
     1081 cgactctttc tccggcgcat tacccggggt tcctggtatc gggtctggcg agtttcttcg
     1141 ggggctcgat gatcggtatt ccctttttgt tagacctatt ccgtattcct gcagacatgt
     1201 ttcagctttt cgtgaccgtg gatgtcttca ccgggcgctt ggggaccatg acagccgcca
     1261 tgcacatgtg ggtgcttggt cttcttggaa gttgcgccct ggctggaaaa ttgcgggttc
     1321 gatggagaaa actagcgggc tatctgactg tctgtttcct gacgggcatc gtccttctcg
     1381 gaggaacacg tctgtttttt acctatgcga tcgagtccga atacaccaag tatcaatcct
     1441 ttgtcgaaat ggcattgaga tttgaacctg cagaaaccgg gattcgcgag ttgactcccg
     1501 atgagaaaac cgttgcaccg tccgaaaaca cggacatgga agccatacgg cagaggggcg
     1561 tgcttcgagt cggatttttc aagaacagtc tgccgtttgt gttccagaat gagacgagcg
     1621 aactagtagg attcgacatc gaaatggccc acttgctcgc caatgatctc ggagtgaatg
     1681 ttgaatttgt ccggcttccg gatcgggaca agattgtgga atatttaaac gacaatgttt
     1741 gtgatatcgt gatgtccggt tcagcgctca gaccggaaat gaccgaaagt gtaatttttt
     1801 ccgacggcta catggatgcg acaatagcct tcctggtgag agatcatgac catgatgtct
     1861 ttagttcgtg ggaaaaaatt cggagtgctg gtgatttgaa gatcggagtt tcctctcatt
     1921 cacagtacta tcaagccttt gcccgaagtt tgttgcccca ggctacgtta atacctgtca
     1981 attcgccgcg tgagttcttc aaggaacacg gaaaggattt tgacgcactc atgtatatcg
     2041 ccgaagccgg ctcggcttgg accttaatct acccggctta ttcggtggca gtccctctac
     2101 ccaaccccat caaaatccct cttgtgtatc ccatgcccaa aagtcatcgg aactttgcgg
     2161 attttgtgaa tgcctggctg agattaaaaa aacgggacgg aacgcttgac gcggtttttg
     2221 aacattggat tttgggcagg ggggcaaaga agaaaactct ccggtggtcc gtcatccgtg
     2281 acgtcctgca ctgggtgaat taaccgggat tgcacctgct gactcctgaa tgtccagact
     2341 cagcgttcag aagagttgtc aagcaggtga catcaggaca atccaagaaa ccgtgaaaaa
     2401 caccggtcga tgggaaccca ttcgcatccg gatgtattgc gaggacggcg aggaaagcgt
     2461 cggctgcgaa attacctttg gcctctacat tccccacagt ttttggagtg gatggcaaat
     2521 tccggtttga ttgaaacaag acaggcttcg gaagcacgtg ggatttccac gatgactctg
     2581 ggagtgatgg gaacaatagc tcgcttcgta ccggcgccgc ctttcagatc aaccaggacc
     2641 tgtaatggga ttttcccggt atgacttcaa cttcaaagga gaccgccgtt tcgacgaaga
     2701 cttgtactta cacgttgtaa ttcatcacgg gctttcccta gtcaggatga atacgggtaa
     2761 ttttggagcc tttgatcccg atacccccca tcaacccttt ctgatcaaaa atgaatgcat
     2821 agatgtcgtc tttcgcagtt gtggtcgtca tggatcctgc aaaacccttg tccacgacga
     2881 cgaggctcgg gcccaccccg atttcccatc catcactttt gtcgagataa tcaagtgcct
     2941 catcctgcat aaaaaacaac acatagccaa aagattggac accggcctgt aatccatatg
     3001 aagcctcaac cgtacggtaa taccctgcgg tctttccatt ttccagaagc gcaccgtcgc
     3061 caaaacttgc acctaccaaa aaaccagcct tatagacgtc cggaaaaaca agaattccct
     3121 tggcgaccgc gccaaagtct ttggcggttt tgctatgcgc atagagtttg gccagggctt
     3181 cttgaacttc cgaattaatt tcagctgcgg tcattgcctg tgccggtgcg aggccaatgc
     3241 ccggaacgat aaggaccatg aagaccagtg ttcgtgcgat tctgtgaata gccttg
//
LOCUS       DAORAQ010000271         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4043_length_3288_cov_67.503557, whole genome shotgun sequence.
ACCESSION   DAORAQ010000271 DAORAQ010000000
VERSION     DAORAQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_3288_cov_67.503557"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKM72_14000"
     CDS             <1..552
                     /locus_tag="PKM72_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61955.1"
                     /translation="GMGMAVVHGIITGHRGAIVVQSVVNKGTKIEVYLPTVPTQVFEP
                     IHDPKAIQNGKESILFVDDEETIVRLGKELLTQLGYTVEVHTSSIEALRTFSEDPHRF
                     DLVITDQAMPGLTGEALSRELLRIRPDLPIILCTGFSHVISAERAKALGIQGYLMKPL
                     AIRDLVPIIRHVLDKTPSSLAES"
     gene            845..1216
                     /locus_tag="PKM72_14005"
     CDS             845..1216
                     /locus_tag="PKM72_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNP61956.1"
                     /translation="MANILVVDDDRQVCDLLKQALEDKGYTVDCATNGVEGIKEYRNR
                     PADLIILDILMPEKEGLETILDLRREFPQVKIIAMSGGSERAKLDLLDLARRLGAQHT
                     IDKPFQLHAITDLVTQALHEN"
     gene            1246..1824
                     /locus_tag="PKM72_14010"
     CDS             1246..1824
                     /locus_tag="PKM72_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61957.1"
                     /translation="MLYFRTQPDAVFIPFVRQALDHELQAVRGLASHNDVEAWLVEYP
                     DVGTLFTPYSALITLEELLVAHEAAMVYRLTDYHWLLVYECLKSYCLWHNDQIYEGSV
                     SFTMLGGYRFGLVDFETMTNRYFWDHDFMELLYADTDEPECCPCGEHTDRSDIDLSYG
                     LRPHPEKLKLTQVEEIAWCIPEPEECGQWRLP"
     gene            1875..2474
                     /locus_tag="PKM72_14015"
     CDS             1875..2474
                     /locus_tag="PKM72_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNP61958.1"
                     /translation="MEKPADVQFHIHDLLGRRWSPRAFADQPVEREKIQRLLEAARWA
                     SSCFNEQPWVFILATIEHPENHQKLLSCLVEGNQIWAKRAPLLLLTVAKLHFDHNGEV
                     NRHAYHDIGLAVGNLVMQATAMDLSVHQMAGILPDAIRERYALPAGYEAVTGIAIGYQ
                     GDASTLPDGLRERELAPRSRKPLREFVFSETWGQTPEYL"
     gene            2703..>3288
                     /locus_tag="PKM72_14020"
     CDS             2703..>3288
                     /locus_tag="PKM72_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNP61959.1"
                     /translation="MITAIGDVLRRCYERGWITSRDGNCSLRRARSVYLYVTPSGWRK
                     TIVHPEHMVKIKFVAGKLQVAPGTNPSGELHMHYLLTRGMNRTRAVVHVHPTHVVAAI
                     YRGFDLQKICKDFPEIYRYTRIGPTAPALPAISTELGEATAKALGVTEANPAGDYDIV
                     GQANHGVCSMAPDPWSAYEHIERLDHICEIVLKSG"
BASE COUNT          808 a          762 c          902 g          816 t
ORIGIN      
        1 gggatgggga tggccgtggt gcatggaatt attacgggtc atagaggagc cattgtcgtg
       61 cagagtgtcg tgaataaggg gacaaagatc gaagtctatc tcccgactgt tccgacccag
      121 gtctttgagc ctatccacga tccaaaagct attcagaatg ggaaagaatc aattcttttt
      181 gtggatgatg aagagactat tgtgaggctt gggaaggaac ttctcacgca actgggatat
      241 acggtggaag tgcacacgag ttcaattgaa gctctgagga catttagcga agacccgcat
      301 cgttttgatc tcgtcattac tgaccaagca atgccgggat tgaccggcga ggccttatcc
      361 cgtgagcttc tgcggattcg cccggacctc cccataattt tgtgtacagg attcagtcat
      421 gtgatttccg cggaacgagc gaaggcattg ggaattcagg gctatttaat gaagccattg
      481 gccattcggg acctggttcc cattattcgc catgtgcttg ataaaacacc ttcgtcgtta
      541 gccgagtctt gacttatccg aagatgaatc catgggtaag ccagggttct cttggttggg
      601 tggtgcgcgg tgaatcactc cagcagatgc cgggtattcc gggaatgtta ttcaataatc
      661 atgaatacca gaagtggatg gttcattggc gataacgttc gagggctgag ccgggaggtt
      721 ttgagcttgg cccataccat taatttttgg aggagccaca gttggtcgca cagttcgtgg
      781 tggatctgtt cctagggaaa aggaagagga ccatccggag tggggcgtgg aaagagacaa
      841 aataatggca aacattcttg ttgtggatga tgatcggcag gtttgtgact tgttgaagca
      901 ggccttggaa gataagggat atactgttga ctgtgctaca aacggagtcg aaggcattaa
      961 ggaatatcgc aaccgcccag ccgatctgat tattttagac attcttatgc ccgaaaaaga
     1021 ggggcttgag accatactgg atttgcggcg tgaatttcct caggtgaaaa tcatcgcgat
     1081 gtccggtgga agtgagcgag ccaaactcga tttgttagac cttgctcgtc gcttaggagc
     1141 tcaacacacc atcgataagc ctttccaatt gcacgccata acggacctcg ttacacaagc
     1201 ccttcacgaa aattagccag ggtttaccta tttggcccac gagcaatgct gtattttcgg
     1261 actcaacccg atgccgtgtt tatccctttt gtgaggcaag cgcttgacca tgaactgcaa
     1321 gccgtgcggg gtctcgcgtc tcacaatgat gttgaggcct ggttggtgga gtatccggac
     1381 gtggggacgc tgtttactcc ctatagtgca cttatcacgc ttgaggaatt gctggtggct
     1441 catgaggccg caatggtcta tcgattaact gactatcatt ggcttctggt atacgaatgt
     1501 ctcaaaagtt attgtctgtg gcacaacgat caaatttatg aaggatcggt gtcgtttacc
     1561 atgctagggg ggtaccggtt tgggttggtg gatttcgaga cgatgaccaa ccggtatttc
     1621 tgggatcatg actttatgga actgttgtat gctgatacgg atgaacctga atgctgtcca
     1681 tgtggagaac acacagacag gtccgatata gatctctctt atggattacg tccgcatccc
     1741 gagaagctca aattaaccca ggtagaggag atcgcctggt gcattccgga gccagaagaa
     1801 tgtggccagt ggcgtctacc ataagaccga attatcaata agtgaagtgg gggtaatgtc
     1861 cctcaggaga aaccatggaa aaacccgcgg acgtgcaatt ccacattcat gatcttttag
     1921 gccgtcgctg gagtccaagg gcctttgccg atcagccggt agaacgagaa aaaatccaac
     1981 gcttgttgga agcagcacga tgggcttcct cctgttttaa cgaacagccc tgggtcttta
     2041 ttctcgcgac catcgagcac cccgagaacc atcaaaagct tctgagttgt ctggtggagg
     2101 gaaatcaaat ctgggcgaag cgagctccac tccttctgct caccgtggca aaactccatt
     2161 ttgatcataa tggagaggtc aaccgccatg cctatcatga tatcgggttg gcggtgggaa
     2221 acctggtcat gcaagccaca gccatggatc tgtcggttca tcagatggct gggattcttc
     2281 ccgacgccat tcgcgaacgc tatgcccttc ccgccggcta tgaagccgtc accgggattg
     2341 ccatcgggta ccagggagat gcttccacac ttcctgatgg gcttcgtgaa cgggagttgg
     2401 cgccacggtc tcgtaagccg ttgagggagt ttgtgttttc cgagacgtgg ggacaaaccc
     2461 cggaatattt gtaacgcatt gaatcccccc cgatctatgt atcggagcga tccatcccac
     2521 gttcttggac tgcttccctc gctcatcaga agcagtgagt tctctctgta tatctacggg
     2581 atcatgtctg ataaagccag gcatgtgctc tgtgaaaggg taagacgaaa tgaaaacagg
     2641 atggtaaact tgacctgaga aaatgaaggc ctattcaaca gtaatggagg ggaaatgaat
     2701 ctatgattac agccataggc gatgtgttac ggcgatgtta tgagcggggg tggattacca
     2761 gccgtgacgg aaattgtagt ctcagacgag cgaggagcgt ctacctgtac gtcactcctt
     2821 caggctggcg aaagacaatt gttcatcccg agcatatggt caaaattaag tttgtcgctg
     2881 ggaagttgca ggtggctccc ggaaccaatc cttccggtga gctgcatatg cattatctcc
     2941 tgaccagagg gatgaatcgg acgcgggctg tcgtgcatgt gcatccaacc catgtggtcg
     3001 cagccatcta tcgcgggttc gatcttcaga aaatctgcaa agactttccc gagatttacc
     3061 gctacacccg tatcgggcca accgcgcccg ctcttcctgc catcagtacg gaacttggag
     3121 aagccacggc gaaggcctta ggtgttacag aagccaaccc ggcaggcgat tacgatattg
     3181 tcggccaagc caatcacgga gtctgctcca tggcaccgga cccctggtca gcctatgagc
     3241 atatcgaacg gttggatcat atatgcgaaa ttgtcctgaa aagcggcg
//
LOCUS       DAORAQ010000272         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4057_length_3274_cov_56.845915, whole genome shotgun sequence.
ACCESSION   DAORAQ010000272 DAORAQ010000000
VERSION     DAORAQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4057_length_3274_cov_56.845915"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKM72_14025"
     CDS             complement(<1..910)
                     /locus_tag="PKM72_14025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP61960.1"
                     /translation="MDILLLAPHPFYQDRGTPIAVNLVLKVLSERGDHVDVLTFHEGR
                     AVDYPNVSIYRIPAWPFLRNIRPGFSLKKLLCDGLLGVKLILLLCQKRYHLVHAVEES
                     AFMALVVKWILRIPYLYDMDSSLAQQMIEKYPRLAILSQAFTYCERRIVKSASVVLPV
                     CEALVKRIEPYRPLKVILLEDVPLLGESTNSFTAVNHDLDVPGIRILYVGNLEAYQGI
                     DLLLESFAVALREVSTITLFIVGGEKDDRDSYCRKAGKLGVDGHVHFLGPRPLDQLSV
                     YLSQADILVSPRIKGNNTPMKLYSYLA"
     gene            complement(1363..2727)
                     /locus_tag="PKM72_14030"
     CDS             complement(1363..2727)
                     /locus_tag="PKM72_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61961.1"
                     /translation="MRNQNVFRPGMVFLCLAIAYPSSLVLAALTLESSFNLTETYTDN
                     LFYNDKDKEDDFGTLAGPNLELRYDNPDIVLGATYFGRFAAFVNNPDQNRYIQNANIL
                     LDLPFLTKRYKKLTVTIDETMNFTPQLDAFSLSGAQDASTTYSGRGGTDSGGTGGTVG
                     AAGGALGGSGISQGTGGTQGVFTSRASAFSNMSGITVGYAMTPSVTPTFSYKNQYLHF
                     FSSGFQDQLTHIGRFALSYGKSEQTTIIPSYTYLQTDYLGTPTQVTVSDKLISHQPRL
                     TISHNFTPLLTGALQGGAAFSKQIGAKETQDNGATTQELSDKWQSTFIGGATITKSYR
                     NGNISLNVDQTIGGGGGLASQETRTQTLTGRIQHSLSTKLNGFASFGYARNKSTQDGN
                     AFDTNTYRVQGGISYAFLNWLSGNVSYSHINQKSKGLAVGDIKVNQVFFGLTAFADPW
                     VLMR"
BASE COUNT          960 a          753 c          738 g          823 t
ORIGIN      
        1 tggcgagata ggaatacaat ttcatcggag tattattgcc tttgattctt ggcgacacca
       61 aaatatccgc ttgcgaaaga taaacagata attggtcaag aggccgtggc cctaggaagt
      121 gcacgtggcc gtcgacacct aattttccgg ctttccggca atatgaatct ctatcgtcct
      181 tttccccacc aacaataaac aacgtgatcg ttgagacctc cctcaaggcc acagcaaagc
      241 tttccaacaa caaatcgatg ccttgatagg cctcgagatt tccgacatat aaaattcgta
      301 tgcctgggac atccagatca tggttcaccg ctgtgaagga atttgtcgat tctccaagaa
      361 gggggacatc ttcaaggagt atgactttta acgggcgata cggttcaatt cgcttgacca
      421 atgcttcaca gacaggcagc acgacagagg cagacttgac gatccgacgt tcgcaatatg
      481 tgaatgcctg ggacagaata gccagacgag gatatttctc aatcatttgt tgtgccaacg
      541 aagagtccat gtcatagaga tagggaatcc gtaatatcca cttcaccacc agcgccataa
      601 aggccgattc ttccacagca tgaaccaggt gatatcgctt ttgacaaagc agcaagatca
      661 acttgacgcc cagcaatcca tcgcacagca actttttcaa cgaaaatcct ggtcgaatgt
      721 tgcggaggaa cggccatgcg ggtattcggt aaatactgac gtttggatag tccacagccc
      781 ttccttcgtg gaacgtaagc acatcaacgt gatccccccg ttcggataag accttgagga
      841 caagattgac tgcaatggga gtccctcgat cctgataaaa aggatggggt gctagcagca
      901 aaatatccat atcagacttt catgaaattc ttaataagga aatccgtagc gaatgtttac
      961 gattattcac acctgggttt ccttaccgag ggattaagcc tcacgaaaat cgggatcagg
     1021 atttttctca gccatgactt gaccactagg attcctacct gttgccaatt accaaacctt
     1081 ccacaggttc taggtttaaa agagaaaatc ctaaagttac ccagacaaaa cacttttaga
     1141 aatctatcgg ttaattcgcg agacgaattg aaggtttgtc acgaagaaga tgagcaaatc
     1201 cacaggcggg cgcggttaga ggcgtggtca ctgaatttgt attcagacaa ccgattgagc
     1261 atattgatgg gataaggaat ggtgcgggca ggatgaagct tgatgcgatt ggcaacgaaa
     1321 ggattttttg gtcccaattc tgaacatttt ctggacccat cctcatcgca tcagcaccca
     1381 aggatcagca aaggccgtca acccaaaaaa gacctgattg actttaatat caccgaccgc
     1441 caggcccttg gacttctgat taatatggga gtagctcaca ttgccggaaa gccagttcag
     1501 aaaggcataa gagattcccc cctgaacccg ataggtattg gtatcaaagg cattgccgtc
     1561 ttgagtcgat ttgttcctcg cgtaaccgaa agacgcaaag ccatttaact ttgtgctcag
     1621 gctgtgttga atccttcccg taagggtttg tgtgcgagtc tcttgagagg ccaaccctcc
     1681 accacccccg atagtttgat ccacattcag ggaaatgttt ccgtttcggt agcttttcgt
     1741 aattgtcgcc cccccaataa atgtgctctg ccacttatca gaaagctctt gggtggttgc
     1801 cccattatcc tgagtctctt tagcacctat ttgtttgcta aacgcggccc cgccctgtag
     1861 agctcctgtc aacaacggag tgaagttatg ggaaatggtt aatcgaggct ggtgagaaat
     1921 cagtttatcc gaaacggtga cctgggtcgg tgtgccgaga tagtctgttt gcaaataggt
     1981 ataggaaggg atgatcgtgg tttgctcaga cttcccatag ctcaatgcaa atcttccgat
     2041 atgtgtcaac tgatcctgga aaccactgga aaaaaagtga aggtactggt ttttgtagct
     2101 aaaagtcgga gtcaccgaag gcgtcatggc atagcctacg gtaattccgg acatattcga
     2161 aaacgcactg gctcgggaag taaacactcc ctgggtgcca ccggttcctt gtgaaattcc
     2221 agatcccccc aaagctcctc ctgcggcgcc cacagttcct cccgtgcctc cagaatcagt
     2281 tccccctctc ccagaatacg tagtagaagc atcctgtgct ccagacaaag agaaggcatc
     2341 taattgagga gtaaaattca tagtttcatc aatagtcacg gtcaatttct tatatcgttt
     2401 agtaaggaaa gggaggtcca acagaatatt cgcgttttga atatagcgat tttgatcagg
     2461 attgttgacg aatgccgcaa acctgccgaa atacgtcgca cctaacacaa tatctggatt
     2521 gtcatatctc agctcaagat tagggcctgc taaggttcca aaatcatcct ctttatcttt
     2581 atcattataa aatagattgt ctgtataggt ttcagtaagg ttgaaggagg attccagagt
     2641 aagagcagct aaaacgagac tcgaagggta agcgatagcc aagcaaagga aaaccatacc
     2701 tggacgaaag acgttttgat ttcgcatgtt ctaaatcaca tcctcctcat ctggtcaaac
     2761 agaaactacc ccaagccctg caaaaatctg gcactgtcgc ctcccaacct caatacaagg
     2821 gaaacaaccg ataaaaccgt gcataatgtt atgtatcggt caaaaagatg aaaaaataag
     2881 ccacaagtcc aaaaataatt cagtctcaat attgggccat gaaaaattct ttctgacttg
     2941 actgcagttc acgacgagaa acaactattt cccccgagaa ctgatgaggt ggcttaattt
     3001 gtggctcact tcagaaggtt taaggagatt tgcaataaag atgtgggcct actattggaa
     3061 ggacatgggt gcgttagaca attggaggaa taaatgtaaa ggtcccctac taatgcccct
     3121 tagcacgaaa aaaattatta aatagaggat gcgcgtgatt tcctgacagg caccgtctac
     3181 gaattaggag gtaatgagat tgcgatacgg cccgaaggaa aaatcctgaa tgagttgaat
     3241 gagtcgacca tgaacctttt ctagattcaa gtaa
//
LOCUS       DAORAQ010000273         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4096_length_3250_cov_72.858842, whole genome shotgun sequence.
ACCESSION   DAORAQ010000273 DAORAQ010000000
VERSION     DAORAQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4096_length_3250_cov_72.858842"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PKM72_14035"
     CDS             complement(<1..1511)
                     /locus_tag="PKM72_14035"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HNP61962.1"
                     /translation="MKNPRQAVLHNVQLLFLLIFFVAGSSSAGSEFENRHHNRDRHYD
                     RDRHHNGDGDQITQPKFANPPQESSIGGILDTELVVNRHEKVVAGRRVLLRSYNGLYI
                     PQTLRVNPGDTLYLRLINKLSDDLLEHTNLHTHGWNVSPQPGSDDIFMQVPADGQFNY
                     SISVPDNHPQGLYWYHPHGHLPQLNPDAPTRGNTEFQIMSGMSGGLIVDGILDPFPEL
                     KGIQEQILLLKDIQITDEGTIPLQIDSNAGTTRMVNGLVNPRMKIRPGELQFWRVGNI
                     GPDIYYKLTLDRHEFFQIARDGNRQNRIWRREEILLPPSSRVEFFVRGEKPGIYTLRT
                     QALTTGPAGDQYPEATLATLVSSGHPDHTMRIPRNLPPVEDFRDAQPIPPGKRRSFVF
                     SEDNDGNSFFVNEKQFNINRVDFTVPVGFIEEWTIQNVTKEFHVFHIHQTDFQVVEIN
                     GVQQEFIGHQDTVNIPFQTGDVPGEVKVLIDFRDPIIVGKFVAHCHIGAHEDNG"
     gene            2544..>3250
                     /locus_tag="PKM72_14040"
     CDS             2544..>3250
                     /locus_tag="PKM72_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61963.1"
                     /translation="MGLLKKLGGMFNRRKRISTNPLYEIVLTYLQKDIHDSPHESIQN
                     LSQASKEKILQEIYNVTETLWQAPDRVLANREGLLESMLHQVDCEIFMIEPGHKLAGF
                     NGISGELKDFLPEFAQKRIDTGELDWKQKTSPTKDEAYKLVWSKWLRANQYCKIFNEI
                     RLYLKDYHTNQERDWFFPLQCASAAFAEYNFRKEYGLTQIIDGTRAQQYGSFLEIVSK
                     GHKDPLEEWEKTYNELFP"
BASE COUNT          898 a          753 c          714 g          885 t
ORIGIN      
        1 ccattgtcct catgggcacc aatatgacaa tgcgcaacaa atttgccgac gataatagga
       61 tcccggaaat caatgagcac cttgacttct ccgggaacgt ctcctgtttg aaatggaata
      121 ttcacggtat cctgatggcc gatgaactct tgttgtactc cgtttatttc cactacttgg
      181 aaatcagttt ggtgaatatg aaagacatga aattcctttg tcacattttg tatcgtccac
      241 tcttcgataa atccaactgg aacggtaaag tccacccggt tgatattgaa ttgtttctcg
      301 ttcacaaaaa acgagttgcc gtcgttgtct tcggaaaaga cgaaggatct tcgcttgcct
      361 ggtggtatgg gctgagcgtc cctgaaatct tctacaggag gcaagtttct gggaattctc
      421 atggtatgat cgggatgtcc tgatgaaacc aatgtggcca gcgtcgcctc cggatattgg
      481 tcgccggcag gcccggtggt cagcgcttgg gtacgcaagg tataaattcc aggtttttct
      541 cctctaacga aaaactcgac acgggaactc ggcggcaata agatctcttc acggcgccat
      601 attctgttct gacgattacc atccctggca atttgaaaaa attcatgccg gtctagcgtc
      661 aatttgtaat aaatgtcggg gccaatattt ccgaccctcc agaactgcaa ctcgccggga
      721 cgaattttca tccgtgggtt aacgagacca ttcaccatgc gtgtagtacc ggcattgcta
      781 tctatttgta gtggaatggt gccttcatcg gtaatctgga tgtccttaag aagaaggatt
      841 tgttcctgga ttccctttaa ttccgggaaa gggtccagaa tgccgtctac aataagtcct
      901 cccgacattc cactcatgat ttggaattca gtgttccccc tggttggtgc atcgggattc
      961 agttgtggca aatgtccatg cgggtgatac caatacaatc cctgaggatg gttgtccgga
     1021 acggatatgc tgtaattgaa ttgcccgtca gccgggactt gcatgaaaat gtcatcactg
     1081 ccgggttgtg gggaaacatt ccatccatgg gtatggagat tggtatgttc cagaagatcg
     1141 tcagacaatt tgttaatcag gcgtagatag agagtatcgc cgggattgac tcttaacgtc
     1201 tggggaatat acagcccatt atacgatcgc agcagaaccc ttcggcctgc cacaactttt
     1261 tcatgtcggt taacaaccag ctctgtatcg agtattccgc caatgctcga ctcctgggga
     1321 ggattggcaa attttggctg agtgatttgg tcgccatcgc cattatgatg tcgatcccga
     1381 tcataatgtc ggtccctatt atgatgacgg ttttcaaatt cggaaccggc tgacgacgaa
     1441 cccgcaacaa aaaaaatcag caagaacaat aactgcacat tgtgtaatac tgcttgtctg
     1501 ggatttttca tacacgttct cctttcccaa ggccatgcaa agcattattt cctttgtgag
     1561 aagtcccgga ttttcgagcc gccgtgttac aacaatgccc gtcaacaaaa acgatctgaa
     1621 cttatatctc cagattcctt gctcccttcc ccacgcacgt ccatggaaag aaaataaaaa
     1681 gtagatcaat agaatacagc tttgggaatg tcaagttgac tgaatcttga tgggcaactg
     1741 tgccgtaaat ccaacacccc tctattgggg tattttctaa taagaaagct cacaagtaac
     1801 cagggccagt tcccgtttga gccgaattct cgatcgtgag cgtcccgatt tcctgaaggt
     1861 ctataattcc gcactctgct ccatactctg tgccccttcg cggcaccatc gccttggtaa
     1921 cacagcattg ctgctaagat cccgggtaac tttcatcacg gcgccatccg attgacatgt
     1981 gaggtgaacg atctccttcc tacactcctg gttcgtacgt cctccgtatc tccctgatct
     2041 acctcaccac aaatgaggct attccaacgc ccccgaaaga caggagaaaa acccggaagg
     2101 aaaagaatag tctttgtcgc aggaagatca aaaaaactga agaattaatc ttgttgaaga
     2161 agttagagag atttttttcg gttcgttcat tatgtgtagt ggaatcgctg ggcactatat
     2221 cacccatttc cccaaagaaa cctaggttga acctccctgc ttcctttatt cgactacatt
     2281 cattctctgg ccattaagac aaatcctggc tatattaaaa taagtagctg gtaaggccgc
     2341 tactactcac taaccattgg atagcctcga tgacatggaa tagaaagcca attttttgct
     2401 caatcccact ctgccagaaa ccagtagaca gcaaataaat tcgtgagagc attaccagag
     2461 gttggaaaag atttcatgtc gattccttat cttcatgaat ctccgggtat gggaaaacta
     2521 ctccttactc gtggaggtct tgtatggggt tgttaaaaaa actagggggt atgttcaatc
     2581 gcagaaaaag aatatccacc aatcccctat atgagattgt tctgacttat ttacaaaaag
     2641 acattcatga ctcacctcat gaatccattc aaaacctttc tcaagcatcg aaggagaaga
     2701 tccttcagga aatttacaat gtgacagaaa ccctatggca ggctcccgat agagtattgg
     2761 ccaaccgcga agggttgctt gagtccatgt tgcaccaggt ggattgtgaa atatttatga
     2821 ttgagcctgg ccataagcta gccggtttca atggtatttc tggcgaattg aaagacttcc
     2881 tgcctgaatt tgcccagaaa cgcatagaca ctggcgaatt agactggaag caaaaaacga
     2941 gcccgacaaa agatgaggcc tataagttgg tgtggagcaa atggttgagg gccaatcaat
     3001 attgcaaaat atttaatgaa atacggctgt atttaaagga ttatcatacc aatcaggagc
     3061 gagactggtt ctttcccctt caatgtgcct cggcagcttt tgcagaatat aattttcgca
     3121 aagaatatgg gctcacacag atcattgatg gtactcgagc ccaacaatat ggttcctttc
     3181 ttgaaatcgt ttcaaaaggc cacaaagacc ccttagaaga atgggaaaag acctataatg
     3241 aattattccc
//
LOCUS       DAORAQ010000274         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4098_length_3247_cov_72.076128, whole genome shotgun sequence.
ACCESSION   DAORAQ010000274 DAORAQ010000000
VERSION     DAORAQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_3247_cov_72.076128"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1965
                     /gene="dnaX"
                     /locus_tag="PKM72_14045"
     CDS             145..1965
                     /gene="dnaX"
                     /locus_tag="PKM72_14045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNP61964.1"
                     /translation="MEFQVSARKYRPTNFKEVLGQSHVVQTLTNAIDRKRVAHAYVFS
                     GMRGVGKTTVARILAKSLNCEQGPTSHACGTCPSCVEITRGNSVDVIEIDGASNTGVD
                     DVRELRENVKFAPFHGTYRVYIIDEVHMLSNSAFNALLKTLEEPPNHCVFIFATTEVH
                     KIPATILSRCQHFTFRRISRLEIIAQLRHVATQTGVTVEDRSLSALARASEGSMRDAL
                     SLLDQAVSFGGERIQHSDLEMLIGSVPDELVRLMIDAILGHQAAQTMSLVGQLVDQGF
                     DVRVYCRELLERCRNLLVACVVPSRSQVQTLLELGDEEVDQAIAQAQQFSEPYLQALF
                     AIFSRTEDGLRGSSHPRFLIEAAVVRAALLDPVTVTSTTTEQKANAPAPSVSHAKVLP
                     TAASTPSPAQPAKVQVPAEKTVAPPSRSRPSGGATTMFPSSTPPGAEKAASTTVSSDN
                     GEPKASPNSAAQHSSDSPMTLNWELVVERMINDHPNIGSFLEKGSVVSMSAGSIVLGY
                     SKKDSIARWRTDKPENRVLIGQICEEFAGRPVKVQVIEFQEGQAYPPSTSELRAKKKL
                     EQDQDLIENVKAHPLVKQALELFGGEVVSAERTPQKEEVR"
     gene            1965..2291
                     /locus_tag="PKM72_14050"
     CDS             1965..2291
                     /locus_tag="PKM72_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNP61965.1"
                     /translation="MSKNPFSNMGNLLKQAQAMQEKMGKIQEEAATKSVEASAGGGMV
                     TVEANGAMQLTKLTIDPEVLKSGDHDMLQDLIVAASNEALRKAKELMAEEMKSLTGGM
                     GIPGMF"
     gene            2311..2949
                     /gene="recR"
                     /locus_tag="PKM72_14055"
     CDS             2311..2949
                     /gene="recR"
                     /locus_tag="PKM72_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNP61966.1"
                     /translation="MLETTPTKHTRANSQPQGLVARLSRELVRLPGIGQKTAQRLAFH
                     LMKTDKEEALRLADAIRDVKEGVSFCTQCRNIAEGPLCEICLDPKRDTTTILVVEEPS
                     TLHAIEQSRAFRGLYHVLYGALSPLDGIGPADIRTHDLENRVQNGEFKEVIVATNPTI
                     EGEATAIYLTKRLKPLGVKVSRIAYGIPVGMDIEYADEVTLLKSIEGRRDLG"
     gene            complement(2963..>3247)
                     /locus_tag="PKM72_14060"
     CDS             complement(2963..>3247)
                     /locus_tag="PKM72_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61967.1"
                     /translation="EFKRLRAVFRHLDLPSQEKDNLLRLSQGFTIPKLFADGVGQDKV
                     SGILSSLISFASAEGGYEKKWRDEIRQVGIWLGYFPQQFEQIETKVLAKR"
BASE COUNT          821 a          912 c          825 g          689 t
ORIGIN      
        1 aagcaacggt acgtgggcta gtctgtgtgc cgcaggatca cctggccccg actaacttta
       61 tgggctgcct ctccgtatct tgataccaga ggtcagtctt cgagggtgct caaggaaaaa
      121 cccttttcct ggttaacgca cattatggaa tttcaggttt cagcacggaa ataccgcccg
      181 actaacttca aagaagtcct ggggcaatct catgtcgtgc aaacacttac caatgcgatc
      241 gaccgcaagc gggtagccca cgcctatgtc ttttccggca tgcggggagt cggcaaaacc
      301 accgttgcgc gcatcctggc caaatccctt aattgtgaac agggcccgac cagccacgcc
      361 tgcggaacct gcccaagttg cgtggaaatc accagaggca attctgtcga tgtcattgaa
      421 attgacggtg cttccaatac cggcgtggac gatgttcggg aacttcggga aaacgtcaag
      481 tttgccccgt ttcatggcac ataccgggtc tacatcatcg acgaagtaca tatgttgtcc
      541 aactcggcct tcaacgccct cctcaaaaca ctggaagaac cgccgaatca ttgtgtcttt
      601 atttttgcca caacggaagt tcataaaatt cccgctacca ttctttcgcg ctgtcagcac
      661 ttcacctttc gacgcatttc tcgcttagaa atcatcgccc aactacgcca cgttgccact
      721 caaaccggcg tgactgtcga agatcgaagc ctatcggccc tggctcgcgc cagcgaaggc
      781 agtatgcgag acgcgttgag tctgctggat caggccgtct ccttcggtgg agaacgcatc
      841 cagcatagcg atttggaaat gctgattggg tccgtgccgg acgaactcgt ccggctcatg
      901 attgacgcca ttcttgggca ccaggctgcc caaacgatgt ctctcgtggg tcagttagtc
      961 gaccaggggt ttgatgttcg ggtgtattgc cgggaactgt tggagcgttg ccgcaatctg
     1021 ctggtagctt gcgtggtgcc ctcacgctca caagtgcaaa cgcttcttga attgggagat
     1081 gaggaagtcg atcaggccat cgcacaagcc caacaattct ccgagcccta tttacaggct
     1141 ctgtttgcca ttttctcgcg aacagaggac ggactccgag gaagttctca tccacggttc
     1201 ctgattgaag ctgcagtagt acgagcagca cttcttgatc ctgtgacagt gacttccacc
     1261 acaacagaac agaaggccaa tgcacccgcc ccatctgttt cccacgcaaa agtattgccg
     1321 actgcagcat cgactccgtc tcccgcacaa ccggctaagg tccaagtacc tgcggaaaaa
     1381 acggtggctc ctccaagccg atccaggccc agtggcggcg ccacgacgat gtttccttcc
     1441 tcaacaccac ctggtgcgga aaaggcggct tcaacaacag tgtcatctga caatggcgag
     1501 cccaaggcca gccccaactc ggctgcccag cattcgtcag actcacctat gacgttaaat
     1561 tgggaactcg tcgtggagcg catgattaac gaccatccga atatcgggag tttcctggag
     1621 aagggaagtg tcgtgtccat gagcgcaggc tccattgtcc tgggttattc caaaaaagat
     1681 tcgattgctc gctggcggac ggacaaaccg gaaaatcgcg tgctgatcgg tcaaatatgc
     1741 gaggaattcg ccggccgccc ggtaaaagtc caagtcattg aatttcaaga agggcaggct
     1801 tacccgccgt caaccagtga attacgagca aaaaaaaaac ttgagcagga ccaggatctg
     1861 attgaaaacg tgaaggccca ccctttggtg aaacaagcat tggaactatt cgggggggaa
     1921 gtcgtatcgg cagaaagaac cccgcaaaag gaggaggtac gctaatgtct aaaaatccat
     1981 tctctaatat gggcaatctt ttaaagcaag cccaggccat gcaggaaaaa atgggaaaga
     2041 ttcaagagga agcggccacg aaatccgtcg aggcttcagc cggaggcggg atggtgaccg
     2101 tggaagccaa cggcgccatg caattaacca agctgaccat cgatccggaa gtactgaaat
     2161 ccggtgacca tgacatgttg caagacttaa tcgtcgccgc atccaatgaa gccttgcgaa
     2221 aggcgaaaga actcatggct gaagaaatga aatctctcac gggagggatg ggcattccgg
     2281 gcatgttcta atcatcaaac gaggtttatc atgctggaaa ccactccgac caaacatacc
     2341 agggccaata gccaacctca ggggctggtc gctcgtctca gccgggaact cgtgcggttg
     2401 cccggtattg ggcaaaaaac ggctcaacgg ttggcctttc atctcatgaa aacggacaag
     2461 gaagaggcgc ttcgcctggc cgatgccatt cgtgacgtga aagaaggggt ctccttctgc
     2521 acccaatgcc ggaatattgc cgaaggtccc ctctgcgaaa tttgcctgga cccgaaacgg
     2581 gacaccacga ccatcctcgt ggtggaagaa cccagcaccc tccatgcgat cgaacaaagc
     2641 cgggcctttc gaggcctcta tcacgtgctc tatggggcgc tttcaccgct ggacggcatc
     2701 gggccggcag atatccgcac ccatgacctg gaaaaccggg tccagaacgg tgaattcaag
     2761 gaagtcatcg tggccaccaa ccccacgatt gaaggagagg ccaccgccat ttacctgacc
     2821 aagcgactaa aacccttggg ggtcaaggtc tcccgtatcg cctatggcat tccggtcggc
     2881 atggatatcg aatatgccga tgaggtcact ctactcaaat ccattgaagg gcgccgcgat
     2941 ctcgggtagc cccagcaccc cgttaccgtt tcgctaaaac cttcgtttca atctgttcga
     3001 actgctgggg aaaatacccc aaccatatcc caacctgtct gatttcatcc cgccatttct
     3061 tctcatagcc cccttcagcc gacgcaaacg aaattaaact cgacaggatt ccactgacct
     3121 tgtcctgtcc tacgccatcc gcaaacaatt tcggaatcgt gaacccttgc gacaagcgaa
     3181 gaaggttatc tttttcttgg gagggaagat ccaaatgacg aaagacggca cgaagccgtt
     3241 tgaattc
//
LOCUS       DAORAQ010000275         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4112_length_3241_cov_63.020088, whole genome shotgun sequence.
ACCESSION   DAORAQ010000275 DAORAQ010000000
VERSION     DAORAQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_3241_cov_63.020088"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKM72_14065"
     CDS             <1..1011
                     /locus_tag="PKM72_14065"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNP61968.1"
                     /translation="HPPPGPWYRSRQLVALARSCLSPLPALPLFTRQPELTRRIRAIA
                     RSQPFDAALINGGDMLWAITELPPEMPTVLIAHNLEHQVLTQKLANYPFLSHVLKREI
                     SKQRQYEIEGFRRSRGVIFLSSTEMAWSCDQVPGLRTLHIPPLFTNPPVIRQPQLNSP
                     LRLGFLADFAWWPNRQSWKWVMENILPKVRRPITVHVFGRQSDQIPTCDNVISHGFVK
                     DLREVWEQVDIMICPIREGAGVNIKLAESLYNRMPVLATNQAVRGLTCSSGSGLVTID
                     SAEEWVTFLNSSEAERLATQVPSEALSQQFAIDQYREKLNNFILEVVVDGLRNQEQST
                     KP"
     gene            complement(1457..3061)
                     /locus_tag="PKM72_14070"
     CDS             complement(1457..3061)
                     /locus_tag="PKM72_14070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNP61969.1"
                     /translation="MSNQLMSKHEITHVTGLTRTLRALILVGMVCIFLPSQGLAYKIE
                     IGVSGPIGGDSVAEENGRTEIQLDTGPVIGPDGEAGSAFATANLATGTLALKVTSTTP
                     DASSQSGMGLASIHIDDHLTFKLDPTVSVANIKFSMSVDGFPSCQDLSSNCFTNAIIR
                     LGDVSDQALGLPLPEPKVLEVRVKVVDGQELPIEAFLSARVEMNNLIDVSDPSATIRL
                     ILPKGVSFTSESGVFLGLQAPSEEPSPRAPVNSDLNGDGKADIVWRNVRTGEVAVWLM
                     NGGLIGSSGFLGGVSSDWQIVGVGDVDNDGMSDIVWRNSLNGAVAVWFLNGLTITSAS
                     FPGSASLDWVIKGVGDMDGNGSADLIWHNTQNGAVAVWLMDGTSISRFRSLPGVPLAW
                     QIVGIGDMNADGLADIIWRHTGGVSAVWVMDESTIASVGFLGGTAPEWEKAGFGDLNG
                     DGTTDMVWRHTRTGKVGIWLMNGGAIEVSGFLAGMPSGWRIAQVGDADGDGKADVIWH
                     NRSTGQVAVWLMDGLTVSSTEFPGSI"
BASE COUNT          823 a          954 c          739 g          725 t
ORIGIN      
        1 cacccgcctc caggcccctg gtatcggtcg cgccagctcg tggcgttggc ccggtcgtgc
       61 ctctcccccc tgcccgcctt gccgttgttt acccggcagc cggagttgac gcggcgaatc
      121 cgcgccatcg cccgctctca gcccttcgat gccgccctga tcaacggcgg ggacatgttg
      181 tgggcgatca cagaactccc gccagaaatg ccgaccgtgc tcatcgcaca taatctggag
      241 caccaggtgc tgactcagaa gctggccaat taccccttcc tttcacatgt cctcaaacgg
      301 gaaattagca aacaacgtca gtatgagatc gaggggttcc gtcgttcgcg aggagtgata
      361 tttctttcgt caactgaaat ggcttggagt tgcgaccagg tcccagggtt gcgaacgctt
      421 catattccgc ccttatttac taatcctccc gtgattcgtc agccgcaact caacagccct
      481 ctgcggcttg gattccttgc cgatttcgca tggtggccca atcgccaaag ttggaagtgg
      541 gtgatggaga atatccttcc caaggtacgc cgcccaatca ccgttcacgt ctttgggcga
      601 cagagtgacc agatacccac ttgtgacaac gtgatctccc atggctttgt caaagatctt
      661 cgagaggtat gggaacaggt ggatatcatg atctgcccca tacgcgaagg agccggtgta
      721 aacatcaaac ttgcggagag tctgtacaat cgaatgcccg ttcttgcgac caaccaggct
      781 gtacgtggcc tcacatgctc atccggatcg gggctggtga ccatcgatag tgctgaggaa
      841 tgggtgacct tcttgaattc gtccgaagct gaacgacttg ccactcaagt cccatcagag
      901 gcattaagcc aacaattcgc tatcgaccag tatagggaga agttgaataa cttcatacta
      961 gaggtggtcg ttgatgggct gaggaaccaa gagcagtcca caaaaccatg atcctgattc
     1021 gactatggac acatgacaaa tcgtatgggc aatacccgtt tttcccatgg cacgactccc
     1081 acagatccaa cctgatttgt tgggatctat cacctgcctc atttctcaca ctccatacaa
     1141 aaaaccgaat acccccatgc ccagtaggga agcaatacaa actcggaatt gacccaccac
     1201 aattacggag aaccttgacc aatagccctt ttcaaaaaaa atttgattaa gggacagccc
     1261 attaggaatc tcttgcgaga ttttcctctt tcatgattat ttttgttgaa aagaacaatg
     1321 aggtgagtgg gaacttgcag aagagcgaaa caaagaactg tatagacaga tcctggtgat
     1381 tcaagctcaa ggggattggg ttgaataaga tcggttgtga aggaggacta cgcgtagcgt
     1441 aaacgcccca ttcggtctaa atgctgccgg ggaactctgt cgaggacacg gtcagtccat
     1501 ccatgagcca gacagccact tgtcctgtac tgcgattatg ccagatcaca tcggcctttc
     1561 catcgccgtc ggcgtcaccc acctgtgcaa tacgccaccc cgaaggcata cctgccaaga
     1621 agcccgacac ttcaatcgcc cctccgttca tcaaccagat ccccaccttc ccggtacggg
     1681 tatgacgcca gaccatatcg gtcgtcccat cgccgttcaa atcaccaaaa cccgctttct
     1741 cccattccgg tgcggtccct ccgaggaagc ccactgaggc aatcgtcgac tcatccatca
     1801 cccacacggc ggataccccg ccggtatggc gccaaatgat gtccgcaaga ccatccgcat
     1861 tcatatcacc tatcccgacg atctgccacg ctaatggcac gccgggcaag gaacggaatc
     1921 ggctaattga ggtcccgtcc atgagccaga ccgcaaccgc tccgttttgc gtattgtgcc
     1981 agatgagatc ggcactgcca ttgccgtcca tatcgcccac tcccttaatg acccaatcca
     2041 aagaagcgct cccagggaaa ctggccgacg taatcgtcaa cccgttcaaa aaccagacag
     2101 ccaccgcacc attgagtgaa ttgcgccaga caatatcaga catgccgtca ttatccacat
     2161 cacccactcc aacgatctgc caatcggatg aaaccccacc caggaatccg gacgaaccaa
     2221 tcagaccgcc attcatcaac cacaccgcca cctcacctgt tctcacattg cgccagacaa
     2281 tatccgcctt gccatctcca ttgagatcgg aatttacagg ggcacgggga gagggttcct
     2341 ccgaaggagc ctgaagaccg agaaacaccc cagattctga ggtaaagctg accccctttg
     2401 gcagaatcaa tctgatcgtc gcacttggat cgctgacatc aatgagatta ttcatctcca
     2461 ccctggccga caaaaatgcc tcaatgggta gctcctgtcc atcaacaact ttgaccctca
     2521 cctccaacac ctttggctcc ggtaaaggaa ggccgagggc ctgatcactc acatctccca
     2581 gacgaatgat cgcattggta aagcaattgg acgacaaatc ctgacaagag ggaaacccat
     2641 cgacgctcat ggaaaactta atattcgcaa ccgagacggt gggatccagc ttaaatgtta
     2701 aatgatcatc gatatgaatg gaggccagtc ccattcccga ttgtgagctg gcatcgggag
     2761 tcgtcgaggt cactttaagg gcgagtgttc cagtcgctaa attggctgtg gcaaaagcag
     2821 agccggcttc accatccggt ccgatcactg gacccgtgtc taattggatt tcagtacgtc
     2881 cattttcctc ggcaactgaa tcccccccaa tcggccctga tacaccaatc tcgattttat
     2941 aggcgagacc ttgtgacgga agaaatatgc aaaccattcc taccaggatg agagccctga
     3001 gggtcctggt cagaccagtt acgtgggtta tttcgtgctt ggacatcagt tgatttgaca
     3061 taagtgatta catgagcaac gctgactacc cttatcggat agacaactcc attcttaaaa
     3121 ttcgtgtggc acagtccaga tcttccctcc ctcgccattt ctgtattctc tcaagactcc
     3181 ttaaaaacgt tggaatttct ccctcgcata gaacaccaat agaggcattc ccctattatt
     3241 t
//
LOCUS       DAORAQ010000276         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4160_length_3215_cov_67.448734, whole genome shotgun sequence.
ACCESSION   DAORAQ010000276 DAORAQ010000000
VERSION     DAORAQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4160_length_3215_cov_67.448734"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKM72_14075"
     CDS             complement(<1..673)
                     /locus_tag="PKM72_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61970.1"
                     /translation="MQHPMKDHSGWKPSLLLFSCLSFAVCVAPVCAGEQNPVQTNNSQ
                     ANKQSNDNENQAGAQEKQNTLLSSHRMITGVVEGVAADQAKVNSGDSGEISPRYLSLE
                     RAKEKGFTLKQGDKVNIVVNAKNHVVDYHLSKEKGQSYHKVIKGRLAQPLKVGQDHAV
                     IKTEEGKEQSFPVRPLARSEVAAIPLDTDGLFLIDETNKIASATLTNDVIAEDDWARS
                     TAYNVY"
     gene            complement(889..1473)
                     /locus_tag="PKM72_14080"
     CDS             complement(889..1473)
                     /locus_tag="PKM72_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61971.1"
                     /translation="MNNFASKFSVQIFSSLLLVSGMMLLPGTAMADEGMGTTSSALGS
                     EGSVPEVQSTPSSGADVQWSDQLKIERDQAKKSRDQAQNDRDTAKETRDQALQASKSQ
                     NTPSGTKSIQGQSRSAQTGEQSHDQEVASLIQERDKYLKERDALQQETATIRKERDRA
                     MHSGGSGFEAVDRTLDAVDRTLDAVDRLPITPRQ"
     gene            complement(2101..>3215)
                     /locus_tag="PKM72_14085"
     CDS             complement(2101..>3215)
                     /locus_tag="PKM72_14085"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNP61972.1"
                     /translation="EFIRQLLADDLQSIDPSLHRHYVVLGSTETEQEVQISPYGPNLL
                     LVGSSGSGKSTLSTGIIERLTDKHYQCCIIDPEGDYESLEQAVVLGDMNHIPNTKEVI
                     NILENPDTNLVINLVGLPIQERPSFFQNIFKDLQDFRARTGRPHWIVLDETHHLMPAE
                     WDTPFPTPSPHTLLMITVHPDQMAADALAAIDCVVVIGKSPGERLEAFTNSVGESLPS
                     FEDHPLQPGEAIFWKRSAGTLPVRFRIHPNRGERRRHRRKYAEGELGPDRSFYFQGPE
                     RKLNLRAQNLMLFLQLAEGLDPETWMYHLKRHDYSSWAQDCIKDKTLAAEIREIESTE
                     SLEHGQSLAHIKSAIESRYVLSPHPSSPPPPSPLST"
BASE COUNT          776 a          767 c          799 g          873 t
ORIGIN      
        1 ggtacacatt ataggcggtg gaacgcgccc aatcgtcttc ggcaatcacg tcattagtca
       61 acgtcgcact cgcaatttta tttgtttcat cgatgagaaa aagtccatct gtatccaatg
      121 gaatagcagc gacctcactt ctggccaggg ggcgtaccgg aaacgattgt tctttgccct
      181 cttcggtttt aatcaccgca tggtcctgtc cgaccttcag cggttgcgca agtctgcctt
      241 tgatgacttt gtggtaactc tggcctttct ctttcgagag gtgataatcg acaacgtgat
      301 tcttggcgtt taccacaatg ttcaccttat cgccctgctt cagcgtgaat cctttttctt
      361 tcgctcgttc aagggagaga tatctaggac ttatttctcc ggagtccccg gaattgactt
      421 tagcttgatc cgctgccact ccttccacaa cgccggtgat catacgatgg ctcgaaagta
      481 acgtattctg tttttcctgg gcgcctgcct ggttttcatt gtcgttggac tgcttgtttg
      541 cctgactgtt attcgtctga acgggattct gttcaccggc gcaaacgggt gctacgcaca
      601 cagcaaatga caaacatgaa aacaagagga gacttggttt ccatccggag tggtctttca
      661 tgggatgttg catcgttgac cctttcgatc attttgcggc cggttgccga gccccatgca
      721 aaatgattat gcagaagttc gaaaaccagc cacacaaaaa atttttaagc tcaagatccc
      781 ggagaatttt atttggctca taaatccccg caacgttctg caagctcatt taacgagaac
      841 tgtaatgtat ggctccgtat aataatccgt tcctggcggc ggagattatt attgtctggg
      901 tgttatcgga agacgatcaa cggcatcgag tgttcgatcc acagcatcca gagtacgatc
      961 aaccgcctcg aacccagaac cgccggagtg catggcccga tcacgctctt ttcgaatagt
     1021 ggctgtctcc tgttggaggg catcgcgctc cttcagatac ttgtctctct cctggataag
     1081 gctggcgact tcttggtcgt gactctgctc acctgtctga gcactacgac tttgtccttg
     1141 aatagatttt gtgcccgagg gggtattctg gctcttgctg gcttggaggg cctggtcgcg
     1201 tgtctctttg gccgtgtctc gatcattttg agcttgatca cggctcttct tagcttgatc
     1261 ccgctcaatt tttagctggt cgctccattg cacgtcagcg ccagaactgg gggttgactg
     1321 tacctccggc accgagcctt ctgaccccag tgccgaagaa gtcgtaccca tgccctcatc
     1381 ggccatggcg gttcctggca gaagcatcat gcctgaaacc agcaggagac tggaaaatat
     1441 ctgaaccgaa aattttgacg cgaaattgtt catgatagca acctcttcat taaggtgatg
     1501 aatttgtctc gccctttggt ttgggatttt aaggtcgaat attgtaaaaa ctcggcattt
     1561 tgatcgatag ggatgaaaag agggagttta ctcataaaag ttcgagtaac ctttcatttc
     1621 tttctttaat tgaaaatggg gcgaagctct gattaaaggc atagcttcgt ccccgatttc
     1681 aaattttcgt tcagacgact aaggcgaatc ggcttaccat gctaatgacc tccaacaatt
     1741 ggaagatcac cacaaatact ccaagcactg tttgtaatac tttcatgatt cttgcccccc
     1801 tgttgaatgc gatggtttca cgaaaaaaat ataaaaaact ttcatgacac taaaggtagt
     1861 atgaagaaaa tgaaggagac ggctaactag taaaaacccc tgaacaggcg atatttaccc
     1921 ctaacgttga aatccctatt cagtcagggg gaagaactcc tttctgtacc gctcagaaca
     1981 tccagaacca caggctggct ggagggtttg tcaatattaa tgagaatacg tgagtgatga
     2041 atttgtgcgt ggtgaatgac ttccagccgt ggcacattgt gttttgaggg aaatagaaaa
     2101 ctaagtagat aagggggaag gaggaggtgg ggaggaagga tgtggagaga ggacataccg
     2161 ggattcaatg gcggatttga tatgagccaa actttgccca tgttctagcg attcagtgga
     2221 ttctatttcc cgaatctcgg cggccagcgt tttatcttta atgcaatcct gagcccagga
     2281 agaataatca tgccgcttca aatgatacat ccacgtttca ggatccagtc cttctgccag
     2341 ttgaagaaag agcatcagat tttgagctcg aaggttcagt tttctttccg gaccttgaaa
     2401 gtaaaagctg cgatcagggc ctaattctcc ttccgcgtac tttcgacgat ggcgcctccg
     2461 ctcgccccta ttggggtgta tccggaatcg aaccggcaga gtcccagcgg accgtttcca
     2521 gaatatcgcc tcccccggtt ggagaggatg gtcctcgaag gaaggcaggg attcccccac
     2581 cgaattggtg aaagcctcca atcgctcgcc tggagatttg ccaatcacca cgacacaatc
     2641 aatagcggca agggcgtcgg cagccatttg atcgggatgc accgtgatca tcagcagggt
     2701 atgtggtgat ggggtgggga aaggcgtatc ccattctgcc ggcatcagat gatgggtctc
     2761 atccagtaca atccaatggg ggcgcccggt tcgtgctctg aagtcctgaa gatccttaaa
     2821 gatgttttga aaaaaagaag gacgttcctg tatcggcaga cccaccagat tgatgacgag
     2881 gttcgtatcc ggattttcca gaatattgat gacttccttt gtgttgggga tgtgattcat
     2941 gtcacccagg accacagcct gttctaacga ttcataatcg ccttcaggat cgataatgca
     3001 gcattgataa tgcttatctg tgagacgctc aatgatgccg gttgagagcg tggattttcc
     3061 gcttcctgag gaaccgacca ggaggaggtt cggaccatac ggactgatct ggacttcctg
     3121 ctccgtctct gtggatccca gcaccacgta atgtcggtgt agggacgggt caattgattg
     3181 taagtcgtca gccagaagtt gccggataaa ctcca
//
LOCUS       DAORAQ010000277         3204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4179_length_3204_cov_72.036520, whole genome shotgun sequence.
ACCESSION   DAORAQ010000277 DAORAQ010000000
VERSION     DAORAQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4179_length_3204_cov_72.036520"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(748..1746)
                     /locus_tag="PKM72_14090"
     CDS             complement(748..1746)
                     /locus_tag="PKM72_14090"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNP61973.1"
                     /translation="MSFQFHNLRSGVAAPPSDDLFAQTVLKGLSKSPKCLPSWLIFDD
                     RGSEIFTEITQLKGYHPAQCEYEIFSKQKQAITDILRNQTFRMIELGAGDGGKTQILL
                     QHCIQSQLIFEYVPIDISEGAIKTLLSSLESRFGEGPMSVTGLAADYFDGLRAIGEQS
                     RVRNFVLFLGSTIGNFTLPAAEEFIGKLRECLNEGDYIMIGFDLMKHPKTLYAAYNDS
                     TGVFEKFNLHLLDVINQKLGANFVKERYVQQGQYNPQSHAVESFVYSTRDQVVSIETL
                     DKEFHFEAWEPMQTEHSYKYTQLEIEMLAEKNGCRLVTHLFDTNNHFVNSIWEVKK"
     gene            1883..2920
                     /locus_tag="PKM72_14095"
     CDS             1883..2920
                     /locus_tag="PKM72_14095"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNP61974.1"
                     /translation="MSDLEYCEAQEIFTQFLQQENLDQYVAHMPKCLKPFEDVLDQAM
                     MEAYEDQSSSSTAHLFLQQILYRINRLKLFWYDDLENYTNEDSVFLLSIRKKIATAWQ
                     AWEAQNIDISALKGLDIEAALRERTAEDLNPELSQAGAFYRNDMSPVGYRQLLAIASL
                     DGLVEASQLSRVIGGVGNEIQTMLTKILFEEYGGAKLERKHTSFFSAMLKELGMDPTP
                     EAYFDIVPWEVLANINHSFTVSERKKFFLRYIGSLLYFEISVPAAFQHYKIAGERLGL
                     SEKAIGYWDIHIKEDLRHGQWMLNEVALPLITRYQDSAWEIVMGYDQQRSLSTRAGLA
                     IAKSVQQAENA"
BASE COUNT          828 a          814 c          677 g          885 t
ORIGIN      
        1 tcaaatgagc tacagcaaaa caggaacaag gaaggacaaa caccaacccg cagattctga
       61 ggattgcatg tattgtccgt tgagaaagcc tgtcttctgg cctattctgt ttcttctttt
      121 tatgttcagt cctgttatct tttcttcaaa aatttcgaat acatcctcaa atcctcagat
      181 tgaatcgtcc ccacctgttc tcaaagaagc ccagcctcaa gaggtactcg gtaaggattc
      241 ttcatttcaa cacgttcaac tccagaaccc ggcttctaaa aaatgagcag aaattctttg
      301 ttagttaaaa aatttcagaa aacccgtgac gtcccaagcc tcaagttatc ttgaaggttc
      361 tttacgagtg ccagtcctaa tttgccagtt tttaactttt cttccatgtg ttgtctccac
      421 gcactgaatg gccctaacaa tcggtacagt ttaagtgttt agtggaaatg tttttcttct
      481 gaaagactcc aaacccattt ctggcatgtt ttgtaacgag gtcgaaccct ctttcctgat
      541 gtggccactg ttgatactta attatggtaa ataactggtc agtaacccca tgcactcgac
      601 tgagtttctt atagcggcat ggcccctttt tgtctattga ttcgaccacg tcaaagaatt
      661 ttccttatac tctcgccgga accagaaggg atccacacct aaacctcctc ttttaccact
      721 aaccgtgaag cagcaactcg atggcaacta tttcttcact tcccagatcg agttcacaaa
      781 gtggttgttg gtatcaaaca gatgcgttac gagcctgcac ccattctttt cagccaacat
      841 ttcaatctct aattgcgtgt atttatagga atgctcggtt tgcattggtt cccatgcctc
      901 aaaatgaaac tccttatcca gtgtctcaat ggacacaacc tggtcacggg tactgtaaac
      961 aaagctttcg accgcatgag attgtggatt atactggccc tgttggacat accgttcttt
     1021 cacaaaattt gcccctagtt tctgattgat gacatcaaga agatgcaggt tgaatttttc
     1081 gaaaactcct gttgaatcat tataggctgc atacagggtc ttcggatgct tcataagatc
     1141 gaacccgatc atgatatagt ccccttcatt caaacattcc cgcagctttc caataaactc
     1201 ctcggccgcc ggcaacgtaa aattcccgat ggtcgaaccc aaaaagagca caaagtttcg
     1261 gaccctcgat tgctcaccaa tcgcgcggag tccatcaaaa taatcggcgg ccagtcctgt
     1321 caccgacata ggcccttctc caaatcgtga ctcgagagaa gatagaagtg tcttgattgc
     1381 gccctcggaa atatcaatcg gcacatactc gaatatcagc tggctctgta tgcagtgctg
     1441 aagaagaatt tgggttttcc ctccatctcc cgcccccagt tcaatcatcc gaaaagtctg
     1501 attcctgagg atatcggtga tcgcctgttt ctgcttgctg aaaatctcat attcacattg
     1561 agcgggatga tagcccttca gttgggtgat ttcggtaaag atttcgctgc ctcgatcatc
     1621 gaaaatcagc cacgatggca ggcatttcgg acttttcgac agccccttca gcacagtttg
     1681 agcgaacagg tcatcagatg gcggtgctgc aactcccgat cggagattgt gaaattgaaa
     1741 tgacattagc ttccttcttc catggtgaaa atgattggta tttgctcccc ctgctcaggc
     1801 ttgctagtat gaagtcgccc accctttata catccttttt aattttttat cttccctgat
     1861 gagtgtttct ttcgttcgca acatgtctga cctcgaatac tgcgaggccc aagagatctt
     1921 tacgcagttc cttcagcagg agaatctcga tcagtatgtc gcgcacatgc ccaaatgcct
     1981 gaagccgttc gaggatgtct tggatcaggc gatgatggag gcctatgaag accagtcttc
     2041 atcttccacg gcacatctgt ttctccaaca gattctctat cgaatcaacc gattgaaact
     2101 gttctggtat gacgaccttg agaattatac gaatgaggat tccgtatttc ttctctccat
     2161 acggaaaaag attgcaaccg cctggcaggc ctgggaagct cagaacatcg atatctctgc
     2221 cttaaaggga ctggacatcg aggccgcgct tcgagaacgc acggcagaag atcttaaccc
     2281 ggaactctcc caagccgggg ccttctatcg caacgacatg tccccggtgg ggtaccgtca
     2341 attgttagcc atcgcctctt tagatggttt ggtggaagca agtcaattgt cacgagtaat
     2401 tggcggcgtt gggaatgaaa tccaaacgat gctcacgaag attctctttg aggagtatgg
     2461 aggggccaaa ttagagagaa aacatacgtc gtttttttcg gcgatgctga aggaattggg
     2521 tatggacccc acgccggaag cctactttga catcgtgcca tgggaagtcc tggccaatat
     2581 caaccacagc ttcaccgttt ccgaacgcaa aaaatttttc ttacggtata ttggcagtct
     2641 tctctacttt gagatctctg ttcctgccgc attccaacat tataaaatcg ccggagaacg
     2701 gcttggcctc agcgaaaaag cgatcggcta ctgggatatc cacattaagg aggatcttcg
     2761 gcacggtcaa tggatgttga atgaagtcgc ccttcccctg attacccgct atcaagatag
     2821 cgcgtgggaa attgtgatgg gctatgatca gcaacgatcc ctcagcacac gcgccggtct
     2881 ggctattgcc aagtccgtcc aacaagccga gaacgcctaa tcgtatttgc tgcctgtcaa
     2941 cccggcagct ctccagacca gagaacagtc ccaatgactc agccttttct ctaaagaagg
     3001 acaggcatcc tccccgccaa tcaggtcaaa actaatttaa tttattcatt ttcccaatct
     3061 ataaaaccgc tccttcacaa ttttgccgtc agaatatgta tcaaaggtct ctcccatgga
     3121 atcgccgagt tctgccaatc tcgtgtcggg atgagggtga gtcttaaaca gtaaggcgac
     3181 gctgctgtca ttgagacccg catg
//
LOCUS       DAORAQ010000278         3198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4184_length_3198_cov_68.114222, whole genome shotgun sequence.
ACCESSION   DAORAQ010000278 DAORAQ010000000
VERSION     DAORAQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4184_length_3198_cov_68.114222"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1319)
                     /locus_tag="PKM72_14100"
     CDS             complement(249..1319)
                     /locus_tag="PKM72_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNP61975.1"
                     /translation="MWVCPKRSALIVVVTLGVLMASGCSSHSVRTGANSEGDGMASSE
                     DAVDTNRIMGQANGSGEGRGINGGLVNGVKPSQHSNGSNSQDMVNGEDPNSNPEGMQG
                     TQRAFGLGGSNYKHQYPGVDGSDPNHGPVSGFENGMASSQNPDPEAWANAYLGDGKNR
                     REFYGDPSKSGIPMDGSSQGGVNPANWGEAYRKEHGGPSPEYVDPDSSIAMGSGQQGR
                     VSVGIGRTDKNGSIFESNYSGSMGIQRGQVQDIYFAFDSWNISGDAAKFLEEGAQWLQ
                     ANPGKALTIEGHCDQRGTQDYNLVLGQKRADAARTYLVNLGVQPDRIKIVSYGKERPF
                     CQHDNEDCFQENRRNHMVVRVN"
     gene            complement(1708..2730)
                     /locus_tag="PKM72_14105"
     CDS             complement(1708..2730)
                     /locus_tag="PKM72_14105"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNP61976.1"
                     /translation="MGKTILVTGAAGFIGSHAVEALIRRGDQVIGIDNLNDYYDPARK
                     EKNLREVTEHAKTEKWPGTFQFLKGDIRDRELVANLFSDHRFDAVIHLAAMAGVRVSI
                     DDPGLYYDVNVMGTMALLDACVGRIGSQGKRSPSPIFVFASTSSVYGNTKTIPFVPED
                     SCDKPLAPYAASKRASELLGYTYHHLYGLDCTVFRFFTVYGPRGRPDMMAYKVLDNIF
                     TGCEVPLYNNGNMHRDWTYVEDIVSGLTAAVDRPMGYEILNLGRGEPVHLVDFVKGIE
                     QLAGRAAHLVPAPMLDADIAYTFADISKTRKLLGYNPQTSVPEGVAKFWAWYQSEILP
                     ASASKP"
     gene            2984..>3198
                     /locus_tag="PKM72_14110"
     CDS             2984..>3198
                     /locus_tag="PKM72_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNP61977.1"
                     /translation="MLYPITYGIVKDPETLETIDTGTGKDFEAEVVVYNCDCHTYQQV
                     VSLFCQVIPRMTPKKAFELAWQIDHHG"
BASE COUNT          872 a          843 c          657 g          826 t
ORIGIN      
        1 gattttaagt cccttgcgtc tgcctattcc gccactccgg catcgcacag attggggtgg
       61 agactctacc actcattaaa aatcggtgca agattttcag acagaaagat taggagagga
      121 taacctaact attccagcct ttttgtcttt ttggttgaaa aacggatgga caagaaaaat
      181 gaagaggatg cgagtgcttc gcatcctctt attcgaagac cacagagggt cctataagag
      241 ccctactttc aattgacccg aacaaccata tgatttcgtc gattttcctg gaaacaatct
      301 tcattgtcgt gttgacaaaa tggtcgctct ttgccgtagg agacaatttt aatccggtcc
      361 ggttgcacac cgagattcac taaatatgtc cgtgcggcat cggcccgttt ttgacctaaa
      421 accaaattat aatcctgagt ccctctttgg tcgcagtgcc cttcaatcgt caaagccttt
      481 ccaggattcg cctgcaacca ttgcgcccct tcctcaagaa acttggcggc gtcgccagaa
      541 atattccaac tatcaaaggc gaagtaaatg tcctggacct gaccccgttg gatccccatg
      601 gaaccactat aattcgactc aaatatcgag ccattcttat cggttctccc aattcccact
      661 gaaacacgcc cttgctggcc ggagcccatt gcaatgctcg aatcgggatc gacatactca
      721 ggagaaggac cgccatgttc cttcctatac gcttcgcccc agttcgcagg attcactcct
      781 ccttgtgaag agccgtccat gggaatcccg gactttgacg gatctccata aaactcacgg
      841 cgattcttgc catctcccaa ataggcattt gcccaagctt ctggatcagg attctgactc
      901 gacgccattc cattttcaaa tccagaaaca ggtccatgat tagggtcaga tccatctaca
      961 ccggggtatt gatgcttata gtttgagcca cctaggccaa atgctcgttg cgttccctgc
     1021 atcccttcag gattcgaatt tggatcttcc ccattgacca tatcttggga attcgaacca
     1081 ttcgaatgct gagaaggttt gactccatta accaacccgc catttattcc tcttccctca
     1141 cctgatccat ttgcctgacc cataatccta ttggtgtcaa ctgcatcttc acttgaggcc
     1201 atcccatccc cttccgagtt ggccccagtt cggaccgaat ggctactaca ccccgaagcc
     1261 ataaggactc ccaacgtgac aacgacaatt aaagcgctac gttttggaca aacccacatg
     1321 agatcctcct ggtgcaatgt tgactattcg tatcatgaga gaaagaagaa tatttctcct
     1381 tcatctcgaa tactgaatgg cctctgaaag actgccaaaa ttatctttat tcttctaaga
     1441 tggacacgtg taaatccttt tcggcagttc cgtagaaaag ttaaggcttc ccgcaacatg
     1501 cgaaaaaatg tccccttgag catcataccc gaaggaccag atatcattat tcaacgtcaa
     1561 caacaggcac gaaatgggag gaaaccttcc gtcgctcgtt ttcaagcagg aatagaggca
     1621 tagattcccc ggtccagttg aaccggggaa taggaacaga ctctgaaaga atgacagggc
     1681 aaaatggcgg aattgagaaa ttcggggtta gggctttgag gctgaagccg gcaatatctc
     1741 cgattgatac catgcccaaa atttcgcgac tccttcgggc acagatgttt gaggattata
     1801 ccccaatagc ttgcgggtct tggaaatgtc ggcaaaggtg taggctatat cggcatccaa
     1861 catgggcgcg ggaactaggt gcgccgcacg tcctgctaat tgctcaatgc ctttcacaaa
     1921 gtcgacaagg tggacaggtt cacctcgtcc aagattcagg atttcataac ccataggacg
     1981 atccaccgcc gcggtaagac cactgacaat atcttcgaca tacgtccaat ctcgatgcat
     2041 gttcccattg ttatagagag ggacttcaca gccagtaaat atattatcca atactttata
     2101 ggccatcata tcgggacgac cacgtgggcc atacacggta aaaaatcgaa atacggtgca
     2161 atccaaccca tacaagtgat gataggtata ccctagcaat tcacttgctc gcttacttgc
     2221 cgcatacggg gcaagcggct tatcacacga atcctccgga acaaagggaa tcgtcttggt
     2281 attgccatac acagaggaag ttgatgcaaa cacaaatatc ggagaaggtg aacgtttgcc
     2341 ctgtgaacca atccgcccca cacacgcatc caataaggcc atcgtcccca tcacattgac
     2401 atcgtaatat agtccagggt catcaatgga tacccgaact cccgccatcg ctgctaaatg
     2461 gataacagcg tcaaaacgat gatcagaaaa aagatttgca accaactctc gatcgcgaat
     2521 atctcctttg agaaactgaa atgtgccggg ccatttctcc gttttggcat gttcggtcac
     2581 ttctcgaaga tttttctctt tacgcgcagg atcatagtaa tcattgagat tatcgattcc
     2641 aatcacttga tctccacgcc tgatcaatgc ttccaccgca tggcttccaa taaaccccgc
     2701 agctccagtt accagtatcg ttttacccac aaacactcct ttatgttact tagagggttc
     2761 ctatttggca catcattttt tcacgctagc tgtcgaattt ggtacacccc tgcattagtt
     2821 ttcttcggca acaattctgc catgcttgat tcaggaagga caatcatagg tcaagtatct
     2881 ctgacgatca atttcctgat tgttctgtaa tcggggcttc ttcaaaataa cggttcccta
     2941 agcattcact tgagggacct cagctccatt gctacaatgc agaatgctct accctattac
     3001 ctatggaatc gtaaaagacc cggaaaccct agaaaccatt gacacaggaa ccggaaaaga
     3061 ttttgaggct gaagtcgtcg tctacaattg cgattgtcat acctaccaac aagtcgtcag
     3121 tcttttttgt caggtgattc ccaggatgac cccgaaaaaa gcctttgaat tagcctggca
     3181 gattgatcac cacggaag
//
LOCUS       DAORAQ010000279         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4192_length_3193_cov_55.974506, whole genome shotgun sequence.
ACCESSION   DAORAQ010000279 DAORAQ010000000
VERSION     DAORAQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_3193_cov_55.974506"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKM72_14115"
     CDS             complement(<1..670)
                     /locus_tag="PKM72_14115"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNP61978.1"
                     /translation="MNIPLQELAQAIHATIHGSSDIQISGLSHLEGASSGDISFLLKP
                     SFQKAARQSQAAAFIVTEPIPDDPRPQLIVPNPLVAVTTLAQKFFLPPLPPRGIHPTA
                     VSGLDVRIGPDVSIGALVTIGDRVLIGSGVTIHAGVHIGDDASIGDECILYPHVSLLT
                     KCVLGNRVIVHSGAVIGSDGFGYVQHEGRHHKIPQLGHVIIEDDVELGANVTVDRATF
                     GSTVI"
     gene            complement(694..1065)
                     /locus_tag="PKM72_14120"
     CDS             complement(694..1065)
                     /locus_tag="PKM72_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNP61979.1"
                     /translation="MKSSVNKSSQLSQQVYHTLGKYLQRDPKNLHPEDSLRDDLGLDS
                     LQTIELVYEVESAFDLQIPDEDFGRLTTIGAVILYLDERTHAQGNALSDPHPTPPAKN
                     SRPAPVVKKAKTRPTSKESRT"
     gene            complement(1062..2315)
                     /gene="fabF"
                     /locus_tag="PKM72_14125"
     CDS             complement(1062..2315)
                     /gene="fabF"
                     /locus_tag="PKM72_14125"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNP61980.1"
                     /translation="MAVRVVITGLGIVSPIGVGVPTFWKSALHGKSGITAISSFGDFP
                     MESYRSRIAGQVSDFRLPDPSEDKFSSRVDRYAQFALAATREAIQDSGLDLEKEPAER
                     MGVMAGVGMGGMMMGERELTTLYQSRKPHRVHPNFIPTITLNSASGILALAFGAKGPN
                     LTISTACSSSIHSIGQALQAIRLNQADIVIASGADASITPLVFAGFCSLRALSTRYND
                     HPDQASRPFDQGRDGFVMGEGAGTLILESLRHATRRKAKIYAELAGYAATNEAYHMVI
                     PREDGSDIARTVSLALKDAGISPSQVDYVNAHATSTVVGDDVEVKGLRAVFDKRLNAI
                     KVNATKSLIGHTLGAAGAIGTIVSALSIQTGVIHPTVNYDNPDPHCALPGLSTKVQKK
                     SVRVGLVNAFGFGSNNAVLVLKKFT"
     gene            2779..>3193
                     /locus_tag="PKM72_14130"
     CDS             2779..>3193
                     /locus_tag="PKM72_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61981.1"
                     /translation="MNKKELVSQLLAAKKASGKTYDELAAALGLCNVYVAQLFRLQAQ
                     LKKETEGKLVKLVPGLTGNLLDAMREFPLRSYDPAVLQEPHIYRMTEVCAHYGESILD
                     IMHEQFGDGIMSAIDFKLTVEKVKGDKGEDRVVMTW"
BASE COUNT          789 a          768 c          911 g          725 t
ORIGIN      
        1 taatgacggt gctgccaaat gttgcccgat ccacggtcac attggccccg agttcaacgt
       61 catcttcaat gatcacatgg cccaattgcg gaattttatg gtggcggcct tcatgttgca
      121 catagccgaa gccgtcgctc ccgatgacgg cgccgctgtg cacaatgact cgattgccga
      181 ggacgcactt ggtcagtaat gagacatgcg ggtagaggat gcactcatcc ccgatactgg
      241 catcgtcgcc aatatgtacc ccggcatgaa tggtgacccc ggagccgatg agcacgcggt
      301 ccccaatagt gacgagagcg ccaattgaga catccggacc aatccgcaca tcgagtcctg
      361 acacagccgt ggggtgaatg ccacgtggtg gaagaggagg aaggaaaaat ttctgcgcca
      421 aagtggtgac ggcgaccagc ggattcggaa caattaattg cggtcgagga tcgtcgggaa
      481 taggctctgt cacaatgaag gctgccgcct gtgactgacg tgccgctttc tgaaacgaag
      541 gcttcaggag aaaggagata tctcccgagg aggctccttc aagatgagag aggccggata
      601 tctgaatgtc agaggatccg tgaatggtgg cgtgaatggc ttgggctaat tcctgaagag
      661 gaatattcat gattcctgag gaagacgata tcgtcatgtg cgcgattctt ttgaagtggg
      721 acgggttttg gcttttttga caactggagc agggcgcgaa ttctttgccg gtggtgtggg
      781 gtgcgggtcg gagagggcgt tcccttgagc atgtgtccgt tcatccagat aaaggatgac
      841 cgccccgatg gtcgttaatc gcccaaaatc ctcatccggt atttgaagat caaacgccga
      901 ttcgacttca tagactaatt caatggtttg tagcgagtcc aatcccaaat catcacggag
      961 cgaatcctca ggatgcagat ttttcggatc gcgctgaagg tatttaccga gggtgtggta
     1021 cacctgctga ctgagttgag aggatttgtt tacggaagat ttcatgtaaa cttttttaag
     1081 acgagaaccg cattgttact tccaaatccg aaggcattca ccaagcccac ccgaacggat
     1141 tttttttgta ccttagtaga aagtcccggc aaggcacaat gggggtccgg attatcatag
     1201 ttcaccgtgg ggtggatgac accggtttga atggataagg ccgagacaat tgtgccgatt
     1261 gctcctgctg ctcccaaggt atggccaatg agcgatttgg tggcattaac cttgatggca
     1321 ttcaggcgtt tgtcgaacac cgcccgtagc cccttgacct ccacatcgtc accgaccaca
     1381 gtggatgtcg catgggcatt gacgtagtcc acctgggatg gcgaaatccc tgcatctttt
     1441 aacgccaggc tgacggtccg agcaatatcc gacccatcct ctctgggaat caccatatga
     1501 tacgcctcgt tggtggcggc ataccccgcc agttcggcat atattttcgc tttacgacgg
     1561 gtggcatggc gaagcgattc cagaatcagg gtgccggccc cttcccccat cacaaaaccg
     1621 tcccgtcctt gatcgaatgg tctcgaagcc tgatcggggt ggtcattata ccgggtggat
     1681 aaggcacgta atgagcaaaa gccggcaaag accaacgggg taatgctggc gtccgctcct
     1741 gaggcaatga cgatatctgc ctggttcaag cgaatggcct gcagagcttg tccgatagaa
     1801 tgaatgctgg aggaacaggc ggtggaaatg gtgagattcg gtccctttgc cccaaaggcc
     1861 aacgccagaa tgcctgaggc ggaattcagc gtaatggtcg gaataaagtt tggatggacc
     1921 cgatggggct tccgggactg atagagggtg gtgagttcac gctcccccat catcatgcca
     1981 cccatgccca caccggccat gacccccatc cgttcggccg gctccttctc aaggtccagc
     2041 ccactatcct gaatggcttc ccgtgtggca gcaagggcaa attgcgcata gcggtccaca
     2101 cgcgatgaga atttatcttc tgaaggatcc gggagacgaa agtcggaaac ctgccctgca
     2161 atcctggagc gataggattc catggggaaa tccccaaaag atgagatcgc cgtaatccct
     2221 gatttgccat gaagggcgga cttccaaaaa gtaggaaccc cgacaccaat gggggacacg
     2281 atgccgagtc cggtaatgac cacgcgcact gccatagttt tctccaaaaa agaaggttct
     2341 aacctaccag aacctttggt caaataggga gggggcatgg tgcgataaat tctgttttta
     2401 tgctcttttt tcttcatata gagcatttca gtttaggatt tggtgcctgg aagggtcgcg
     2461 cggtaaaggg gaatttgagt tgaataaatt cacgatttgc ctaaaacgca agcagaatgg
     2521 cggaatcatg aaaaatggct ggcggaaatt acttgagtcc ccgcttgaga acttcccctt
     2581 tcgggggaag tctggaaagg catcaaggac tctccagcac gtactggaag ccgtttcagg
     2641 tgggggaata ttggattgac acgtccatgc tcaccgccta acgtattgtc aggcgtgagg
     2701 cagagaagaa cgtctcagct caaggaggac catgggaata tcaatcattc ggagacttta
     2761 aaggaggaga gggtgaaaat gaataagaag gaacttgtca gccagttgct tgcagcgaaa
     2821 aaagcctcag gaaagacgta tgacgagctg gcagcagccc ttggcctgtg taacgtctat
     2881 gtggcccagt tattcagatt gcaggcgcaa ctcaagaagg agacggaggg caagctcgtc
     2941 aagctggtac cgggactcac ggggaatttg cttgatgcca tgcgggagtt ccccctgcga
     3001 tcctatgatc cggcggtctt gcaggagcca catatttatc gcatgaccga ggtttgcgcc
     3061 cattatggag agtccattct ggacatcatg catgagcagt tcggtgatgg gattatgtcg
     3121 gcgattgatt ttaagctcac cgtcgagaag gtaaagggtg acaaaggaga ggaccgtgtc
     3181 gtcatgacgt gga
//
LOCUS       DAORAQ010000280         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4197_length_3189_cov_63.123803, whole genome shotgun sequence.
ACCESSION   DAORAQ010000280 DAORAQ010000000
VERSION     DAORAQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_3189_cov_63.123803"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..663)
                     /locus_tag="PKM72_14135"
     CDS             complement(145..663)
                     /locus_tag="PKM72_14135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNP61982.1"
                     /translation="MASISWISYVVWKVRQDPTRWKSWGFRTDNLRQAFIWPSVLFAW
                     SWLAMVWYAIVNGRVLWQDHMLFLLFLYPLWGILQQFLVQALGVDNLIKIFPQHGLWL
                     AIPIGVILFAVVHFPDWWLMLATGLMACFFIPFYIRDGNLWPLGLYHGWLGTFFYLWV
                     LNRDPWVSVFGK"
     gene            complement(800..1252)
                     /locus_tag="PKM72_14140"
     CDS             complement(800..1252)
                     /locus_tag="PKM72_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61983.1"
                     /translation="MSSKRWSRVMWEEFLSRIFSGDHVSFVPHKIFRWDENGIYRDCF
                     FPVPCQHLLGGEHLIGRATNEVLPKDSARSLKKALTKTLKTQRPQDVQLVFPTLGKTV
                     VAVVRLFPYESDALGFITDHYLDGSPVITVSPQDPSVAFLKGSSAYTL"
     gene            complement(1658..2887)
                     /locus_tag="PKM72_14145"
     CDS             complement(1658..2887)
                     /locus_tag="PKM72_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNP61984.1"
                     /translation="MQTSVVNSGFENEGVSVLASAPSLRHVWSQIDCRSLTPKLEQFL
                     AGRPETPFLALDLEQVRHKYLELQGEFPQAAIHYAVKANPAPEIIQLLATLGSRFDIA
                     SRYELDLCLSLGVPATWLSYGNTVKKESDIGYAFACGVRLFAFDSVAELEKLGRQAPG
                     AGVQCRVLSQGKNADWPLSRKFGCDLAMAYELLIKGCDLGLKPLGVTFHVGSQQTDPQ
                     QWVEPMQQAAELYRQLRRQGLELEWINIGGGLPATYQTTLQPFSHYAQTIQQALQQSF
                     GVTPPQLMIEPGRFLVADAGIIQSEVILVSHKSREDVRRWVYLDIGKFGGLAETMDEC
                     IKYRLRTFRQGPTGPVILAGPTCDSADVLYEKTSYELPMDLAIGDRIEVLSAGAYTSS
                     YASVGFNGFPPLRTYCL"
     gene            complement(2954..>3189)
                     /locus_tag="PKM72_14150"
     CDS             complement(2954..>3189)
                     /locus_tag="PKM72_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61985.1"
                     /translation="SCAPFEASSGSRQSKLQALWCNGTWDFLPHGPKISQTNFPFAIG
                     LEKLCARDQGVWFQAREWFRTPLKISWILSRCI"
BASE COUNT          933 a          853 c          693 g          710 t
ORIGIN      
        1 aaaccgttca tacaaaagct gcaaccgttc ttgatcagaa attacgagcg gattactttc
       61 catagctata catctccctt tacatcgtca acagtggttc tttttctact cccctctcat
      121 caaactcgtc aagtgggtgg cgagctattt tccgaaaacg gatacccagg ggtcgcggtt
      181 aagaacccag agataaaaaa acgtgcccag ccagccatga tagagcccca gcggccaaag
      241 gtttccatca cggatataga acgggataaa aaagcaggcc ataaggccgg tggccagcat
      301 cagccaccaa tcggggaaat gaaccacggc aaacaggatc accccaatgg gaatggccaa
      361 ccacagtcca tgctgaggaa aaattttgat gagattatcg acacccaggg cctgcacaag
      421 aaactgttga aggattcccc acaacggata caggaacaat agaaacagca tgtggtcttg
      481 ccaaaggacc cgaccgttca ctatggcata ccagaccatg gctaaccacg accaggcaaa
      541 taacaccgat ggccaaatga aggcttgtcg aagattgtcg gtgcgaaatc cccaactctt
      601 ccacctcgtt ggatcttgcc gaaccttcca gacaacatag cttatccaac tgatgctggc
      661 caaggctatg aacagtccct ttgcaccaag acttttgaat acaagatgca gcagccctgt
      721 caagacaacc gcagagaatt cccaccaatt gccaacccga tcatcgggag aaattttcag
      781 aaaatctctc ccccatgaat tacagggtat aggccgagga gcctttgaga aaagccacgg
      841 agggatcctg gggggaaacc gtgatcactg gtgaaccatc taggtaatga tccgtaatga
      901 agcccaatgc atcagactca taaggaaaaa gccggaccac agcaacaacc gtctttccca
      961 atgtaggaaa aaccaattga acatcttgcg gccgttgagt tttcagtgtt tttgtcaggg
     1021 cttttttcaa acttctggct gaatctttcg gtaagacctc atttgtggcc cgcccaatca
     1081 gatgctctcc acctaaaagg tgttgacagg gaacagggaa aaaacaatct cgatagatcc
     1141 cattttcatc ccaacgaaag attttatgcg gaacaaacga gacatgatca ccggaaaaaa
     1201 tcctgctcaa aaattcttcc cacatcaccc tcgaccaccg tttactggac atagaaccct
     1261 ccatggtttc tatccatttc ctctatcata taggtcaatg atcattacac caaaacttca
     1321 gggctcttta agagagtaaa taagagttct cttctcatgc acaactaggg aaagtcctag
     1381 ttattccaaa aaaattccct agtatcctat ctactgtatt aaaaaaggaa cgatttagaa
     1441 caacgcttct ttcctctata cctaaaatag acataaattg cctggttcac caggaaggcc
     1501 ctggtccaat tcactcagac tttggtattt gaccacccca ccctgcggcg tcatctgagt
     1561 cagaaaatac tgtaggaaac ccagggaaat aaatcaacgg cctctaaaat gcaataagca
     1621 gcaaagcgag cacacggtcc attgaccagt ctatccgcta gagacagtag gtccgcaagg
     1681 gtggaaatcc attaaacccc acagacgcat agctgctggt ataggcgccg gcgcttaaga
     1741 cttcgattcg atcaccgatc gccaaatcca tgggcaactc ataagatgtt ttttcatata
     1801 acacgtctgc gctgtcacag gtggggccag ccaatatcac cggtcccgtt ggaccctgac
     1861 ggaaggtacg taggcgatac tttatacatt catccatcgt ttcagccaaa ccgccaaact
     1921 ttccgatatc gaggtacacc catcgccgaa catcttcgcg agatttatga gaaaccagga
     1981 tcacttcgct ctggataatt cccgcatccg ccaccaaaaa ccgcccaggc tcaatcatca
     2041 gttgaggagg tgtgacacca aatgattgct ggagagcctg ttggatggtt tgagcgtaat
     2101 gcgaaaatgg ctgaagagtc gtttggtagg tagccggcaa tcctccgcca atattaatcc
     2161 attccagctc cagaccctgt cgtctcaatt ggcgatacaa ttccgctgcc tgctgcatgg
     2221 gctcgaccca ttgctgcgga tcggtttgtt gagaccccac atggaacgtc acacccagcg
     2281 gtttcaaccc gagatcacaa cctttgatga gtaattcata tgccatggcc aggtcacaac
     2341 cgaattttct agaaagaggc caatccgcgt ttttcccttg gctcaggact cgacattgca
     2401 ctccagcacc aggcgcctgt ctccccaatt tttccagttc ggccacacta tcaaaagcaa
     2461 agagtctaac cccacaggca aaagcatagc caatgtccga ctctttttta accgtgttgc
     2521 catacgacag ccaagtggcc ggcaccccga gagacaaaca caaatccagt tcataccggc
     2581 tcgcaatatc aaagcgggag cccagcgtcg ctaatagttg aatgatttcc ggggcgggat
     2641 tggctttcac ggcataatgg atcgctgcct ggggaaactc tccttgaagc tcaagatact
     2701 tgtgtcgtac ctgctcaaga tcaagagcta aaaacggtgt ctcaggacgc ccggccagaa
     2761 attgctcaag ctttggggtc aggctgcggc aatcaatctg tgaccaaaca tgtcgaagcg
     2821 atggagcact tgcgagaaca gaaaccccct cattttcgaa cccggaatta acaacactgg
     2881 tctgcacatt ccactcctat gacgatggtg attaaaaggg atcaaacgcg ccaagcctaa
     2941 agtccaaaag ccttcaaata catcggctaa gtatccagga aatcttaaga ggagtccgga
     3001 accattcacg tgcctgaaac cagactccct gatccctggc gcaaagcttt tccagtccga
     3061 tggcaaacgg gaaattcgtt tgagaaattt tgggaccatg cggtaagaaa tcccaggtcc
     3121 cgttacacca taatgcctga agttttgatt ggcgggaccc ggaggatgcc tcgaaagggg
     3181 cgcaactaa
//
LOCUS       DAORAQ010000281         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4227_length_3175_cov_65.252244, whole genome shotgun sequence.
ACCESSION   DAORAQ010000281 DAORAQ010000000
VERSION     DAORAQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4227_length_3175_cov_65.252244"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKM72_14155"
     CDS             complement(<1..201)
                     /locus_tag="PKM72_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61986.1"
                     /translation="MADSLEQLQKIADDLKRQRDEVQVKLHLAKADARDEWAKLETRW
                     EEVKSKMEAVKKEASQTTESVSS"
     gene            complement(448..852)
                     /locus_tag="PKM72_14160"
     CDS             complement(448..852)
                     /locus_tag="PKM72_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61987.1"
                     /translation="MRTTIPIQACAIGLVLVLVGGEIAEAEMPPPEPDEAYLVDEDIN
                     IVTGLYMREYSLRKDGIVDYKTARQILISEYNEHWNSVVETKEWPLFYWHDVNRDGHW
                     SMYVDRKVNGCTCDIVPYEVKTEDTIAQNDLY"
     gene            complement(1106..2077)
                     /locus_tag="PKM72_14165"
     CDS             complement(1106..2077)
                     /locus_tag="PKM72_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNP61988.1"
                     /translation="MKYVHLGRSGLKVSRLCLGTMNFGPETTEADSGHIMDTALELGI
                     NFFDTADVYGWEVGEGITEQIIGRWFAQGGNRREKVVLATKVFGRMGDWPNQSRLSAL
                     HIKRACEASLRRLQTDYIDLYQMHHVDRHTPWEEIWQAMEQLMREGKVLYVGSSNFAG
                     WHLAQAQEMARHRNFLGLVAEQSLYNLNDRMIELEVIPACEAYGIGLIPWSPVARGLL
                     AGALEPATSGRRADEDLRKNVEKYRPRLEAYETLCREIGEAPAHVALAWLLHQPAVTA
                     PIIGPRTMDQLKGTMRTTEFTLSKDVLKKLDTIFPGPGGPAPEAYAW"
     gene            2324..2872
                     /locus_tag="PKM72_14170"
     CDS             2324..2872
                     /locus_tag="PKM72_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNP61989.1"
                     /translation="MSIQYSIDSTDHIRFINEAWLQFACQNDGAHLSRAYVIGKSLWE
                     FIDGEDNRQIHRLILNTVRATQRVQEIKFRCDSSVCRRYFLLTVSPLPEGELMFSTHP
                     LREDSRSPVPLLDPDVKRGRQYLRICSWCIKACLSNQNWVELEEALSRLDLLGGGIMP
                     NLTHGVCPDCQVTIEKELKNPK"
BASE COUNT          741 a          803 c          795 g          836 t
ORIGIN      
        1 ggaggacacg gattcggtgg tctgactagc ttcctttttg accgcttcca tcttgctctt
       61 gacctcttcc caccgtgttt caagtttcgc ccattcatca cgcgcatcgg ctttggccag
      121 gtggagtttc acctgaactt cgtcacgctg acgtttcagg tcatcagcaa ttttttgaag
      181 ctgttctaat gagtctgcca tgaacgtctc ctttcattat tcgaaccgta tgaataattc
      241 tattctagat taacaagaaa ttagcctaag agtgtacccc gtcacatagg tgaaagggac
      301 caacgttaat cagccttcta actgtgatgt gcaggaaggc aggaaactgg gggatttcca
      361 ggcataggcg ggggtgcgtg gcgcctggtc ctgtgaagcg ggagattatg ttgggtcgag
      421 taacggttga ccagtgcgag tggatattta ataaagatcg ttctgcgcga tcgtgtcctc
      481 agtcttcact tcgtaaggaa cgatatcgca ggtgcagccg ttgacctttc gatccacata
      541 catggaccaa tgaccatccc ggtttacatc atgccagtag aataagggcc attctttggt
      601 ttcgacgacc gaattccaat gttcattata ttccgagatg aggatttgac gggcggtttt
      661 gtaatccacg attccgtctt ttcgaagtga atattcccgc atgtagaggc cggttacaat
      721 attgatatcc tcgtcgacca ggtaggcctc gtccggttcg ggtggcggca tttcggcttc
      781 ggctatttct cctccaacga gaacaagaac taatccgatg gcgcatgctt gaatggggat
      841 tgtcgttcgc atgacatcct ccttggctaa aggccctttg cccgactacc ttggtgtgtg
      901 tgagcttcct aacttattta gagcaactgt tatgccaaag gagaagtggg taattgtttt
      961 gtctggaggg aaaccaacct cttgagttgt aaggaattag aatggtgacg gggcgattga
     1021 gggcagaatg cactcgtcaa gattatgcct atgggttata catttatgca caatattatc
     1081 aatatttgac acctattgat ggctttcacc aggcgtaagc ttcgggggcc ggacccccag
     1141 gtccggggaa gatcgtatcg agctttttta acacatcctt ggagagagtg aattcggtgg
     1201 tgcgcatggt gccttttaac tggtccatgg ttcgaggacc aatgatgggt gccgtgacgg
     1261 ctggttgatg aaggagccag gccaaggcga catgtgcagg cgcttcgcct atttcccggc
     1321 acagggtttc gtaggcttcc aatcgtggac ggtatttttc cacgtttttt cgcagatcct
     1381 catcggcccg tcgtccgctc gtcgccggtt caagcgcccc ggccagcagg ccgcgcgcga
     1441 ccggactcca gggaatcaaa ccgatgccgt aggcttcaca ggccggaatg acctcaagct
     1501 caatcatgcg gtcattcagg ttgtacaaac tttgctcggc caccagtccc aggaaattcc
     1561 ggtgcctggc catttcctgc gcttgggcca gatgccatcc ggcaaagtta ctactcccca
     1621 cgtataacac ttttccctcg cgcatgagtt gttccatggc ctgccagatt tcctcccatg
     1681 gagtatgccg gtccacatgg tgcatctgat acaggtcaat ataatcggtt tggagccgcc
     1741 gcaaacttgc ttcacacgcc cgcttgatat ggagcgcgga taaacgggat tggttgggcc
     1801 agtctcccat ccgcccgaac actttggtgg ccagcacgac tttttcccgg cggttgcctc
     1861 cttgtgcaaa ccaacgcccg ataatctgtt cggtaatccc ttcccccacc tcccaaccat
     1921 atacgtctgc ggtatcgaaa aaattgatgc cgagttccaa tgccgtatcc atgatgtgtc
     1981 cactgtcggc ttctgtggtt tccggaccaa aattcattgt gcccaaacat aaccgactga
     2041 cttttaatcc tgatcgacca agatggacat atttcattca gccactcctc atgaaagatt
     2101 tgggataccg ggaaaaagtt ctttcacacc ctaccaagaa taccgaaact tcttccagaa
     2161 gatggggacc accagccgtg gaatgggtgc ggtgtcgggt gcacggtcta cccaagacgt
     2221 ccctcctgtc gattttgaat tggtttacca tttggcgtaa gatgaaccca cccttcacga
     2281 ttgttgtggg aagtcccata caagcaggag gtcctgcagc gtcatgtcga ttcaatacag
     2341 catcgacagc accgatcata tccggtttat caatgaggcc tggttgcagt tcgcctgcca
     2401 aaatgacgga gcacacctga gtcgtgccta tgtcatcgga aagtcgcttt gggagttcat
     2461 cgatggggaa gataacagac agattcatcg gttgattttg aatacggtta gggccacaca
     2521 aagagttcag gaaatcaaat tccgctgcga ttcttcagtc tgtcgccgat attttctcct
     2581 taccgtgtct cctctccctg agggcgaact gatgttctca acgcatcccc ttcgtgagga
     2641 ctcacggagc cccgttccct tacttgaccc tgatgtgaag aggggaaggc aatatctcag
     2701 gatctgtagt tggtgtatca aagcctgcct ctcgaatcag aattgggtgg aactggaaga
     2761 agctctttcc cgtcttgatc ttctaggtgg cggaatcatg ccaaatctta cgcacggggt
     2821 ttgccccgac tgtcaggtta ccattgagaa ggagttgaaa aacccgaagt gaccaggtag
     2881 aacagtgctg gctctgttca ttgccatgga atcccgtgtg ctcgaaaaaa tctgacatgg
     2941 tggtgtatag tcgtttagcg tattcatgga caaaaggacg caagtctctg tgccccgttg
     3001 ttctttgtga gattcatttt tggttaattg gagggtagta tggccgtaaa aaagaagtcg
     3061 atccgtcaag cacctaacaa acgcactcct tcttccaaca aaaaacagac gccttctcgc
     3121 aaaaaaacac cagcttcctc caagaaagga tcagcctcct ccaaaaaagg gacag
//
LOCUS       DAORAQ010000282         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4284_length_3142_cov_57.839002, whole genome shotgun sequence.
ACCESSION   DAORAQ010000282 DAORAQ010000000
VERSION     DAORAQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_3142_cov_57.839002"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKM72_14175"
     CDS             <1..1213
                     /locus_tag="PKM72_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61990.1"
                     /translation="IPKPLPVCPEEAPVKSFNVVAMDFPGMSFNPSAPESIEIDFERT
                     IELRNPEGKIYVLEDEVTKVAGDYHPMPLTIRANVGDCIKVNLKNKMKESRASFSAIS
                     LAFNPQDSLGANVGNNKGDQTIAPGESKTYTYYADPFNGEISSLVWDWGNVMLNPRNG
                     LFGAVIIGPKGSQYRDPKTGADISLKNSWEADVIVDKTIPGQENRKNYRDVALLFQDE
                     DNIIGTSFMPYVQNVAGLTGVNYRSEPYLHREEAGCTLGRMFQPCEVENPQDPVTPLI
                     QAHAGDPVRIHVFGASSEQNGMFSVEKHEWPIEPFMPGADEISVVEFAGSETIDVFIP
                     SAGGRDMLPADYVWNNQRLPYSQSGQWGYLRVLPKNDQRILPLERGASEIKQASIDAV
                     ETGRVLPVAAK"
     gene            1654..2595
                     /locus_tag="PKM72_14180"
     CDS             1654..2595
                     /locus_tag="PKM72_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNP61991.1"
                     /translation="MRGRQSKKRLVATGLLVIGFTLISVMMAWALDVSDIRPEWTEAG
                     KKLALQRAQWPTKEKRVVIPAGWFTMGSNKKIDRKAYRPEMPQRQVYLDAFEIDAYEV
                     TALEYLRFVVDTGRPPLPDWKFDGGNFQESMAHHPVMHVSWFEADAYCRWAGKRLPTE
                     AEWEKAARGTDARIYPWGNTPAGLTRANFGRSGISGPVRDRPERLLLYPPIISVDKYE
                     IGVSPYNVFQMAGNVAEWVNDWYDPEYYQTAPDRNPQGPSTGEHRVFRGGGWMDSTPT
                     VRTAQRNGKAPEAQANWLGFRCAAGLKNDLTHSISTP"
     gene            2960..>3142
                     /locus_tag="PKM72_14185"
     CDS             2960..>3142
                     /locus_tag="PKM72_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNP61992.1"
                     /translation="MMMRVLVVVIFLMMVSSMGWAYQESQVSNGGAIQGHVRLLGEPP
                     RPMAFNLVTIPDAVYCG"
BASE COUNT          809 a          636 c          862 g          835 t
ORIGIN      
        1 gattcccaag ccccttcctg tttgtcctga agaggctccg gtgaaatcat ttaatgtggt
       61 cgcgatggac tttccaggga tgagttttaa tcccagtgct ccggaatcca ttgagattga
      121 ctttgagagg accattgagc tgagaaatcc tgagggcaag atctacgtcc ttgaggatga
      181 ggtcactaaa gtggcagggg attatcaccc gatgccattg actattcgag caaacgtggg
      241 agattgcatt aaagttaatc tcaaaaataa aatgaaggag agccgggctt ccttctcggc
      301 aatatcattg gcctttaatc cccaagattc attgggggca aatgtcggta ataacaaagg
      361 ggatcaaacg attgccccag gagagtcaaa gacctatacc tattatgcag atccttttaa
      421 cggagagatt tcttccctgg tatgggactg gggaaatgtg atgttaaacc cacgaaacgg
      481 tttgttcggg gcggtcatca tcgggcctaa agggtcgcaa taccgtgatc cgaagaccgg
      541 tgcggacatt tccttaaaaa atagctggga agcggatgtg attgtcgata agaccattcc
      601 aggtcaagaa aaccgcaaaa attaccgtga tgtggcttta ctctttcaag atgaagacaa
      661 cattatcggc accagcttta tgccgtatgt tcagaatgta gccggtctca ccggtgtcaa
      721 ttatcgttct gagccttatc ttcacagaga agaggctggt tgtacgcttg gacgcatgtt
      781 tcagccttgt gaggtagaaa atccacaaga tccggttaca ccgcttatcc aggcgcatgc
      841 tggcgacccc gtgaggattc atgtgttcgg tgccagcagt gagcaaaacg ggatgtttag
      901 tgtggaaaaa catgaatggc cgatcgaacc attcatgccg ggagccgacg aaatcagtgt
      961 ggtggagttc gccggttcag aaacgatcga tgtatttatc ccgtccgctg gcggacgtga
     1021 tatgcttccc gcagattatg tctggaataa tcagcggctg ccttactctc aatcaggtca
     1081 gtggggctat ttaagggtac tcccgaaaaa tgaccaacgg atcctgcctc tggaaagagg
     1141 agcgagtgag atcaagcaag cttcgatcga cgcggtggag actggaaggg tgttgccggt
     1201 ggcagccaaa taaccaactt catcattaag gttttaagaa tgggggattc gcaaggatcc
     1261 cccatttttt tatatttgta ttggatttct tttatcggtt gtgaaacgaa acggatcaaa
     1321 gttttaattt aagccaaatt gaaaatgcca ttaggtaagc tgttatgata tttctacaag
     1381 aagtatccct catttaagcg aatctcggat tatcaattct aatgggtttt tttaagccaa
     1441 ttatggcttt tggcaaatgc cttgacctcg tcgaaaggtg aagctacgat ttgctcaatt
     1501 aaatgaccat actgacataa aaatattggg gttcccagcg aatgccttct tttccgggcg
     1561 aaatgaagtc atccgaattc ggccttgggg tggtgaattc tccaattttt ccatcagtgg
     1621 cgtcttagtc atcggattaa tttggagggg tttatgcgtg gaagacaaag taagaaacgt
     1681 cttgttgcga ccggtttgtt ggtgatcggg tttactttga tatcggtgat gatggcatgg
     1741 gcgttggacg tgtcagacat acgaccggaa tggactgaag ctggtaagaa attggcttta
     1801 cagcgagccc aatggccgac taaagaaaag agagtagtaa ttccagcggg atggtttacg
     1861 atggggagta ataaaaaaat tgatcggaag gcctaccggc ctgagatgcc ccaacgccag
     1921 gtgtacctgg atgcctttga gattgatgcc tatgaagtca cggctttgga gtatttgcgt
     1981 tttgtcgtgg acacgggtcg tccgccatta ccggattgga agttcgacgg aggaaacttt
     2041 caggagagta tggcccatca tcccgtgatg catgtctcgt ggtttgaagc cgatgcctat
     2101 tgccggtggg caggaaagcg gcttcctacg gaagcggagt gggaaaaagc tgccagagga
     2161 acggacgcac gaatttatcc atggggaaat accccggcag ggcttacgcg agcaaatttt
     2221 gggagatcgg gcatttccgg cccagttcgt gatcgacctg aacggctgct tctttatcca
     2281 cctataatct ccgttgataa gtatgaaatc ggggtcagtc cttataacgt ttttcaaatg
     2341 gcagggaatg tcgcagaatg ggtgaacgat tggtacgatc cggaatatta tcagacggct
     2401 ccggaccgga atccgcaagg cccgtcgacc ggtgaacatc gggtatttcg tggcggtggc
     2461 tggatggata gtactccgac tgtgcggaca gcccagcgca atggaaaagc tcctgaggca
     2521 caggccaact ggctgggctt tcggtgtgct gctggattga agaatgatct cactcattcg
     2581 atttccactc cctaatggtg agaaatggat gcgttctcgt tgctgagttg ctctccattt
     2641 cctctcacaa agattattga tgtatttttc aatacttctt actaaacgca ggggacggtc
     2701 ctaatttcag agggtttgag ttcgcaaaag gtttaaatct gaatttctgg tgattcgaat
     2761 gtgatagggt gatatgagag gagtggctca ccaaagaaag ctaccaatca ctatgagatg
     2821 ctcgcaagga tattttaatg ctcgagtgtc ttgtcgtatg tgaccgggag tacttcctga
     2881 ttatagggtt tttgggaaga gggcagatat aacacattga tatttttggg ccggcagtga
     2941 tgagaaggag ggcgttggaa tgatgatgag agttttggtg gtcgtgatct tcctgatgat
     3001 ggtgtcatcc atggggtggg cctatcaaga gagccaggtc agcaatggag gggcaattca
     3061 agggcatgtc cgtttacttg gtgaaccccc aagaccgatg gcgtttaatc tcgtcacgat
     3121 cccggatgcc gtatattgtg ga
//
LOCUS       DAORAQ010000283         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4315_length_3127_cov_63.056641, whole genome shotgun sequence.
ACCESSION   DAORAQ010000283 DAORAQ010000000
VERSION     DAORAQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4315_length_3127_cov_63.056641"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1979
                     /locus_tag="PKM72_14190"
     CDS             309..1979
                     /locus_tag="PKM72_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNP61993.1"
                     /translation="MPMIHGLHFKNIRGDIYGGVVAAVVALPLALAFGVASGAGAIAG
                     LYGAIFVGMFASLFGGTPAQCSGPTGPMTVIMAGILVQFSDNPALAFTVVIMGGGLQI
                     IFGLLGFGYYIALVPYPVISGFMSGIGCIILILQLGPLVGLEAKPEGIVENLMAIPQF
                     LANPLPQAAILGVVTLSIMYLTPVRISQFIPPQLLALCLGTFAAYMFFPQVPLIGHVP
                     EGFPSLIFPDIEPHAFVIMAEGAVVLALLGSIDSLLTSLVCDNMTRTQHNSNRELIGQ
                     GIGNMVAGIFGGLPGAGATMRSVANIRSGGRTPLSGMLTALVLLAMLLGLGPLAEKIP
                     LAVLAGILFKVGLDIIDWRFLRHLLKAPRTDVIIMGVVLVITVLVNLITAVGVGIVLA
                     SLLFVKRMADLELANLRIISAPTTEIPFGPEEKAILERNNGRIVLIHVDGPMSFGSAK
                     NMVRRLESIKGFNNFTHVLLDLSDVSAIDGTAALAIEDMLRMVQAHRQNLYFVGIKAG
                     VLKVLEGLGVLDQIGPEQRYDLRLDALRHADQGDNFLPTNEFSRAQRA"
     gene            complement(2151..2555)
                     /locus_tag="PKM72_14195"
     CDS             complement(2151..2555)
                     /locus_tag="PKM72_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61994.1"
                     /translation="MGFIILLEVAQIFRGERHRLQAHSKLVLVISGMLIVGGTLGLLL
                     LEWDIPKTLGNLPIMDEILVGAFLSVSARAAGFISFGVMPLSTSLSFSSGGVAGGTAG
                     GIKTPTFGIIGRFVCIALPHRQDVNFLPGCQR"
     gene            2851..>3127
                     /locus_tag="PKM72_14200"
     CDS             2851..>3127
                     /locus_tag="PKM72_14200"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNP61995.1"
                     /translation="MKNFSIEKLIQIGYRFVTKSQGHFYLYLAIFCSVLVILDAGSVE
                     LIRGMKLKTFDAIMKNRVLFHKADPDIVIVDIDEASLEAMAQEYGRWP"
BASE COUNT          753 a          738 c          776 g          860 t
ORIGIN      
        1 tcctgattcc cgttatttta tccacataga ggttcaaagc tagatgtggg cccccttgtc
       61 gtattccggc tattggaaat tcagttgctg aaaggcgggg caagaacgcc tcctattcac
      121 agatcaattc tttttcattg cccataatgt tctctgaatt atgtactgta aagaacatct
      181 accgtttaga tacccatttt tcccctctca gatcagtcaa tagtccatgc gcctcaacac
      241 gctagtttgc tcagttgcga taggtgatgg tttcgaaaaa cctcattcat gaaggagtaa
      301 gagagacgat gcccatgatt catggactgc atttcaagaa cattcgagga gatatatacg
      361 ggggtgtggt cgcggcagtc gtcgcccttc ccttggcgct ggcatttggt gtggcctccg
      421 gtgccggtgc gattgccgga ctgtatggcg ctatctttgt cggcatgttt gcctccttgt
      481 tcggaggaac acctgcgcaa tgttccggcc ccaccggccc tatgacggtc atcatggcgg
      541 gtatactcgt tcagttttct gacaatcccg ccctcgcgtt tactgtggtg attatgggtg
      601 gtggattaca aattattttc ggtttacttg gctttggtta ttatattgcg cttgttccct
      661 atccggttat ttcaggattc atgagtggaa ttggatgcat tattcttatt ttacagctgg
      721 gaccacttgt cggattagag gccaaacctg agggaattgt cgaaaatctg atggccattc
      781 ctcagttttt ggccaatcct ctgcctcagg cagcgattct gggtgtcgtc accctctcca
      841 ttatgtattt gacaccggta cgaatcagtc aattcattcc tccccaacta ctcgcgttat
      901 gcctgggtac ctttgccgcc tatatgtttt tcccccaggt gccgttaatc gggcatgtcc
      961 cagaggggtt cccctctcta attttccctg atattgagcc tcatgccttt gtcatcatgg
     1021 ccgaaggagc ggttgtgctg gctctcctgg gcagcatcga tagcctcttg acctcgctgg
     1081 tgtgtgacaa tatgactcgt acccaacata attccaatcg tgaattgatt ggtcagggaa
     1141 ttggcaatat ggtggcaggc atatttggtg gccttcccgg agcaggagcg acgatgcggt
     1201 ccgtggcgaa tattcggagc ggcggtcgta cccctctgtc agggatgctg accgcgttgg
     1261 tcttattggc catgttactg ggattggggc ccttagcgga aaagattccc ttagccgtgt
     1321 tagccggcat tttgtttaaa gtgggattag atattattga ttggagattt ctcaggcact
     1381 tactcaaggc cccacgaaca gatgtgatca tcatgggagt ggtgctggtc attacggttc
     1441 tcgtgaattt gatcaccgct gttggcgtgg gaatcgtact ggccagtctg ttgtttgtaa
     1501 agcggatggc cgaccttgag ttggcaaatt tgcggatcat cagcgcgccg acaaccgaaa
     1561 taccttttgg gccggaggaa aaggccattc ttgaacgcaa taatgggcga attgtcttaa
     1621 tccacgtgga tggacccatg agtttcggat ccgctaaaaa tatggtacgc cggctggagt
     1681 cgataaaagg cttcaacaac tttactcatg tccttctcga cctgtcggat gtctcggcca
     1741 ttgacggaac tgccgctctt gccattgaag atatgctgcg tatggttcag gcccatcgcc
     1801 agaatcttta ctttgttgga attaaagcgg gagtgcttaa agttctggaa ggattggggg
     1861 tcttggatca gattggccct gaacagcgat acgaccttcg attggatgcg ttgcgtcatg
     1921 ccgatcaagg ggataatttt ttacccacaa atgaattctc cagagcgcaa cgggcatgac
     1981 tgaaatatta gcacgccgcc atcgccaatg gagtacccgg ttgttcccaa ggccgacgcg
     2041 acctcaaaca agagagagaa acggatgagg ttcaaccatg gataagaaat gcgtgaacag
     2101 ggttactagg aacatggcga gaaaaattaa agccagagcc cggtgaacga ctatcgttgg
     2161 caccctggca aaaaattcac gtcttgacga tgcggcaagg caatacacac aaaccttccg
     2221 ataattccga acgtgggcgt tttaattccg ccagcagtcc ctcctgctac cccccccgat
     2281 gaaaatgaca atgaggtaga gagaggcatt accccaaatg atatgaagcc tgccgccctg
     2341 gcagaaactg aaagaaaggc ccccaccaaa atttcatcca ttatcggcaa attccctagc
     2401 gttttaggga tatcccattc aaggagtaag agacccaaag ttcctccgac aatcagcatg
     2461 ccggaaatga caagcaccag cttggaatgg gcctgaagac ggtgacgttc tcctcgaaaa
     2521 atttgagcaa cctcaagcag gataataaat cccaaccctc aaaaaacaac caaaatctac
     2581 gtgatgagat tcacggtcag atcggtctga taggagattg aattggttga aaatgaggga
     2641 aatccgggaa ggacaaatca ttaaaaattc cgccccatgt ttcctctgta catttcttca
     2701 gttgttggct accatttcta cttgaatgtt ttaattccta cctttctgat cactcctgcg
     2761 gaatttctgt ttttttgtta acccaagtcg tgggtgccgt gagatgacag cgattcaggc
     2821 caaacccagg tttaaggtct tgctcaagag atgaagaact tttctatcga aaaactcatt
     2881 cagatcgggt atcgctttgt cacgaaatcc caaggtcatt tttatctcta tctggcgatt
     2941 ttctgtagcg ttctcgtgat cttggatgcc ggctccgttg aactgattcg gggaatgaag
     3001 ctgaaaacct ttgatgcgat catgaagaac cgggtgttgt ttcacaaagc cgatcccgac
     3061 attgtgattg tcgatattga tgaagccagt ttggaggcca tggctcaaga atatggccgg
     3121 tggccct
//
LOCUS       DAORAQ010000284         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4334_length_3114_cov_70.207257, whole genome shotgun sequence.
ACCESSION   DAORAQ010000284 DAORAQ010000000
VERSION     DAORAQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4334_length_3114_cov_70.207257"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKM72_14205"
     CDS             <1..601
                     /locus_tag="PKM72_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61996.1"
                     /translation="IMTDGNARADLTEYIYFREFSFGGDLGMVTTTLTFFADPDKETS
                     PIPDFTDSRLNFSWNIRRNQTMPGIDITPAPCSSGCQFDLTDPTSDANLNFSLGSGTI
                     YTIGAQKIILTELRIGPTEGNRPLLFLGNPDLRPPLQTSALGTKGRWTAIPAGSLPGS
                     SSQPIPEPDTAWLMLTGLFGFGTYVYWSRSIRRLFKKTW"
     gene            827..1306
                     /locus_tag="PKM72_14210"
     CDS             827..1306
                     /locus_tag="PKM72_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61997.1"
                     /translation="MMNLKFPARDRRYSFMMGFAMVSMLTMLFGCQQQHRVDSVVIKP
                     NTCPLPEDLSTEDQHKVKKFGAEISGLMSSLAGGKLDTDLQSALQKNYPDAGDVNRIY
                     AMSYAACVSCRVDPNDVKGCAQRFDDIIDNFTAKDTAPTHPAKGYRSKLLNPLRGPK"
     gene            1851..>3114
                     /locus_tag="PKM72_14215"
     CDS             1851..>3114
                     /locus_tag="PKM72_14215"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNP61998.1"
                     /translation="MPTPSSLSEIPIQFAKGVGPRRARLLEKLGVLTLEDAFWFLPWR
                     YENRLEVLPIGNLLPGMKATIKGRVQKVLVKTTSRRGLVVVTVTVRDETGLIECVFFN
                     QPYLEKTFAPGVSLLLTGSVLPNSSGSLPMTMRGPEYELLDEDELPDMSGGRIVPVYH
                     ETNGLSSKQIRRIIRLIFDHYADQLQEMLPTAMRMQLGVPTLPEALPVLHFPNQERSV
                     EALNQQATPEHRRLAFEEMFLLQLALAMRRQGQTQDIEGIAFAVRNPLVEQLKTVLPF
                     SLTSAQERVIQEISQDMGRPTCMNRLLQGDVGCGKTVVALHAIVMACGSGYQAALMAP
                     TEVLSEQHYLSLFPLFEALGIRCLLLKGGQTGRERGKILAGIQSGHVQVVVGTHALLQ
                     PDVNFAKLGLVIVDEQHKFGVLQRAQLRG"
BASE COUNT          735 a          753 c          799 g          827 t
ORIGIN      
        1 aattatgact gatggcaatg cgagggcaga tctcactgaa tacatctatt ttagagaatt
       61 cagtttcgga ggggatctcg gtatggtgac cacgaccctc acgttctttg ccgacccaga
      121 caaggagaca tcccctatcc cggatttcac cgacagtcgt ttgaattttt cctggaacat
      181 tcgtcggaat caaaccatgc cgggaattga tatcacgcct gctccatgtt catcgggctg
      241 tcagttcgat ctcaccgacc ccacatcgga tgctaattta aatttcagcc ttggctccgg
      301 tactatctac acgataggcg cgcaaaaaat aatccttacg gaactaagaa taggtccaac
      361 agaagggaat aggccgctcc tgtttctggg gaatcctgac ctgcgcccac ctctacagac
      421 ctcagccctc ggtaccaaag ggcgttggac agcgatacct gctggttcgt tgccaggatc
      481 aagttcgcag cccattcctg aacctgatac ggcgtggttg atgctcacgg gactctttgg
      541 ctttggaacc tatgtctact ggagccgatc tatccgtcgc cttttcaaga aaacatggtg
      601 aaaaacctgc aagttactta gaagttctag ctggttccgg gactttcatc tttaaaagaa
      661 atgcagaatg ccttcatttt tgcatccgct ttttgcggaa gtcgttcgct tcgattgagg
      721 aagaaccctt cattcatctc tgtcgtggtt gccatccaac gtgcctcaac tcataatgtt
      781 gctcttcgaa cgtatgcttc taacctatct tgacaaagga ggattcatga tgaatctgaa
      841 atttcctgca agggatcggc gttattcgtt tatgatgggc tttgcgatgg tcagcatgct
      901 gaccatgctc ttcggatgtc agcagcagca cagagtcgat tcggttgtca ttaaacccaa
      961 tacctgcccg cttccggaag acttgtcgac agaagatcaa cataaggtca agaaatttgg
     1021 tgcggaaata tcaggtctca tgtctagctt ggctggaggc aagcttgata ctgatttaca
     1081 aagcgctctc caaaaaaatt atccggacgc cggagatgta aatcgtattt atgccatgag
     1141 ttatgccgcg tgtgtctctt gccgggtcga cccgaatgat gtgaaggggt gtgcgcaacg
     1201 tttcgatgac attattgata atttcacggc gaaagacaca gctccgaccc atccggcgaa
     1261 ggggtatcgt tccaaactcc tgaatccgtt gcgaggcccc aagtagtctc ttcccatcca
     1321 taggattggg tcttgcgaaa tcttttcttt ggtgtcctca ccactttttt cagaaggtgg
     1381 ggcatccagt attttcttga tgtgaaaact accgggtatg caatgtgtcc attgcatcaa
     1441 ggtacggata ttctggaact gacttgccaa tagtgatttg aagaggacaa gctcaccaat
     1501 tccttgcaag tttggctgtt gccggtcacc ggaatcgggt tccggaagaa gaccagcgga
     1561 atcgcaaaga caatccacac acaagacggc tattttaaat tgtcggctaa ctcgcctgta
     1621 tcgcgggact acacggctgg caaaaaatga cgcatgtcaa tcttctcttg acaccaatcc
     1681 attctcttcg tacgtttcac aggcaatctg tgatatctgg attaatcgtt ttccaccctc
     1741 aaatttctca taagcccttt tatggagatt gggctggttc gagcacattc ccgcagggtc
     1801 tttcggtcta agacttccgg ttctggtgca ataggctttt tgtaatcatc atgccgactc
     1861 catcctccct ctctgagatt cccattcaat ttgccaaagg ggtggggccc agaagggctc
     1921 gcttgctgga aaaattggga gtactgacat tagaggatgc tttctggttt ttaccctggc
     1981 gatacgaaaa ccgcttggaa gtgttgccca tcgggaacct tctcccaggc atgaaggcca
     2041 ccattaaagg cagagtacag aaagtgttgg tgaaaaccac ctctcgaaga ggtctggtcg
     2101 tcgtcacagt tacggtgagg gatgagacgg gtcttatcga atgcgtgttt tttaaccagc
     2161 cctacctgga gaaaactttt gcgccaggtg tctccttgct attgaccggt tcagtcttgc
     2221 ccaattcttc cggaagccta ccgatgacga tgcgcgggcc ggagtatgaa ttgttggatg
     2281 aggacgaatt gccggacatg tcggggggac ggattgttcc ggtctatcat gagacgaatg
     2341 ggttgagctc caagcagatt cgacgaatta ttcgattaat ttttgaccat tatgccgacc
     2401 agctgcaaga gatgttgccg acggcgatgc gcatgcaact gggggtccca acccttccag
     2461 aggcgttacc tgtcttacat tttcccaatc aggagcgttc tgtggaggcc ctgaatcaac
     2521 aggcaactcc cgagcatagg cggttggcgt ttgaagaaat gtttctcctg caattagcac
     2581 tcgccatgcg gcgccagggg cagactcagg acattgaagg catcgccttt gctgttcgca
     2641 atccgttggt cgaacagctg aaaactgtgt tgcctttctc gctcacttcc gcacaggagc
     2701 gtgtgatcca ggaaattagc caggatatgg gacggccaac ctgtatgaat cgcctcctgc
     2761 aaggagatgt cgggtgtggg aaaacggtgg tggctcttca tgccatcgtg atggcctgtg
     2821 ggtccgggta tcaagccgcg ctgatggcac cgacagaagt gttgagtgag cagcattatt
     2881 tatcgttgtt tccgttgttt gaggccctag gtatccgttg tctcctgttg aaaggcgggc
     2941 aaacaggccg cgagcgaggc aaaattctcg caggaatcca gtcaggtcat gtgcaggtgg
     3001 tggtgggtac acatgcgtta ttacagcctg atgtgaattt tgccaagctt gggttggtga
     3061 ttgtggatga gcaacacaaa ttcggcgtgc tgcagcgcgc acagcttcgt ggaa
//
LOCUS       DAORAQ010000285         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4352_length_3107_cov_84.853211, whole genome shotgun sequence.
ACCESSION   DAORAQ010000285 DAORAQ010000000
VERSION     DAORAQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4352_length_3107_cov_84.853211"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKM72_14220"
     CDS             complement(<1..351)
                     /locus_tag="PKM72_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP61999.1"
                     /translation="MRTAPTYLLRAMLIAVSAFCASCDLLGGAFDHEPKDLEKEMSAS
                     ARALIEQAFDGIHGNARRDYHVHMLGMNEDINRTFVNEEWQSPWSGLTHFFQFEIYKS
                     AAGITDEQQADAKYL"
     gene            1190..1738
                     /locus_tag="PKM72_14225"
     CDS             1190..1738
                     /locus_tag="PKM72_14225"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNP62000.1"
                     /translation="MEHIFFDNWQALFRTGLISILAYVSLITLLRLFGKRTLSKMNAF
                     DLVVTVALGSTLATILLNKDVTLAQGVLALALLIGMQFVVTWSSLRVRWVRKLATGEP
                     TMLLYQGTFLPVALGWTRVTEDEVRAAVRNAGAATLDEVKAVVLETDGSFSVVRRGGG
                     SGESSLIGVNRPSGIPPPHTNR"
     gene            1759..>3107
                     /gene="fumC"
                     /locus_tag="PKM72_14230"
     CDS             1759..>3107
                     /gene="fumC"
                     /locus_tag="PKM72_14230"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNP62001.1"
                     /translation="MTKKTTSARIETDSFGSIAVPADKYWGAQTQRSLENFQIGQERL
                     PRPLIRALGIVKRCAALSNIHLNAIDETTGKAIADAAQDVIDGKLDDHFPLVIWQTGS
                     GTQSNMNANEVIANLAIERLGGTLGSKTPVHPNDHCNRSQSSNDVFPTAMHIATVEQL
                     HHVLLPSLEHLHEALMTKSQAWKDIIKIGRTHLQDATPLTLGQEFSGYAAQVQLGIDR
                     IQDGLKRLYPLAQGGTAVGTGLNAKPGFGEKFAEEVAKYTQLPFLSASNKFEALATHD
                     AMVEISGVLNVIAVSLNKIANDIRLLSSGPRSGLGELSLPENEPGSSIMPGKVNPTQC
                     EAMTMVCAQVIGNHTTITISGAQGHLELNVFKPVIAYNVLQSIRLLGDAVMSLTDKCI
                     VGIEPNIARISELMEQSLMLVTALVPHIGYDKAAKIAQGALKNGRTLREEAIASGFVT
                     "
BASE COUNT          756 a          739 c          824 g          788 t
ORIGIN      
        1 aaggtacttg gcgtcggcct gttgttcgtc ggttatccct gctgcactct tatagatttc
       61 aaattggaaa aaatgagtta atccgctcca cggtgattgc cattcctcat tgacgaaggt
      121 tctattgatg tcctcattca tgcccagcat atgcacgtga taatcgcgac gggcattacc
      181 gtgaatgccg tcgaatgcct gttcgatgag cgcgcgtgca ctggcgctca tctctttttc
      241 caaatccttg ggttcatgat cgaaggcgcc ccccaaaaga tcgcaggaag cacagaaggc
      301 gctcacagca atcagcattg cccggagaag atatgtcggt gccgttctca tatgcttgtg
      361 tggaaccata ttgagtgcat ctggttcgtg gagacgtggt tgatctcact gacagacgat
      421 attgggtatg gaacgaaagg ttttaggaaa gtacacacgt aatttcttta aacaaagaat
      481 gcccactcta tgttggcttc ctttgaggga aaaagggggg tggatggatt tgccttgtgc
      541 atctttcggg aaggcagaat ggtaaataag catttcattt caaccctatc gtaaaaaatg
      601 gtttaaggaa atattttttc atgctttttg gggtatttct gaaggcttgg tttacaaact
      661 ttataccttg actaattttc agaattaacc aagaatcttt ccattcatgc acatgtgaaa
      721 aaacaatttt gactacagcg attctgtggt ggaagaaggt ttatgactaa ttcggcaaaa
      781 tcggaaactt ggccccccct aattctgaac gagtgggagg atacatatgg aaccttgcac
      841 atgtggatgc agatcgtcgg caaggttcgc ttgacccgga ccccctggat cgatctgata
      901 cacttcttga attcctgcaa ataatgtatg aagccgcagc caatttaata aaatgggatc
      961 gcgcctcttt tgaaccaagg atcgatccca aaattacagg ggcatgagaa tccggccggg
     1021 ataatgctcg gatctttgct ggctcggtcc ggtgttcccg tcttcatctt ggaaaagcat
     1081 gggaatttcc tgcgtgtctt tcgtcgatag aaaatagtta gtagttccct caatggaggc
     1141 gagtgtttaa acttcaattc tgctggcgac agaaagcagg gaggtgcaag tggaacatat
     1201 cttctttgat aattggcagg cgctgttccg aacgggattg atcagtatcc tggcatatgt
     1261 cagccttatc acgcttttga ggttgttcgg caaacgtacc ctctccaaga tgaatgcctt
     1321 tgatttagtc gtgacggtcg cgcttgggtc gacgctggca actattttgc tcaataaaga
     1381 cgtgaccttg gcccagggag tcctggcctt ggctctgctg ataggaatgc agttcgttgt
     1441 gacgtggtcc agtcttcgcg tccgatgggt gcgtaagctg gccacagggg aaccgacaat
     1501 gctgttgtat cagggtacat ttttacctgt tgctttgggg tggacacggg tgaccgaaga
     1561 tgaggtgcga gccgcggttc ggaacgcagg agcggcgacg ttggatgagg tcaaggcggt
     1621 ggtgttggag accgacggct ccttcagcgt agttcgaagg ggtggaggtt ccggagagtc
     1681 cagcctcata ggggtcaacc gaccgtcggg tattccaccc ccacatacca acagataagc
     1741 tatgccgtta gaggagccat gacaaagaag acaactagcg cacgtatcga aaccgactcc
     1801 tttggttcga ttgctgtgcc agccgacaaa tattggggtg cacagaccca acgttccttg
     1861 gaaaattttc aaataggaca ggagcgtttg cctcgcccat tgatccgggc gttggggatc
     1921 gtcaagcgtt gcgcggctct atcgaatatc cacctgaacg ccattgatga aacaaccggc
     1981 aaggccattg ctgatgcggc acaggatgtg atcgacggca agttggacga tcactttccc
     2041 cttgtgatct ggcaaactgg ttccggcacg cagtccaaca tgaacgccaa tgaggtgatt
     2101 gccaatctgg ccatcgaacg tctgggcggg acgctcggat caaaaacgcc ggttcatccg
     2161 aatgatcact gcaatcgcag ccagtcttcc aatgatgttt ttcccacggc catgcatatt
     2221 gccacggttg agcaactgca ccatgtcctg cttccatccc tggagcattt gcacgaagcg
     2281 ttgatgacga aatcacaggc atggaaggac ataatcaaaa tcggacgaac ccatttgcag
     2341 gacgccactc ctctcaccct tgggcaggaa ttctcaggat atgcggccca ggtgcaattg
     2401 ggaattgatc ggattcagga tggccttaaa cgactgtatc cccttgcgca aggcggtacg
     2461 gctgtgggta cgggattgaa cgcgaagccc gggtttggtg agaagtttgc tgaagaagtc
     2521 gcaaaatata cgcagctgcc ctttctttcg gcttccaata agttcgaagc gttggccacg
     2581 catgatgcca tggtggaaat ctccggcgtg ttgaatgtga tcgccgtcag tctgaataaa
     2641 attgccaatg atattcgcct gttaagttcc ggtccgcggt ccggtcttgg agaactctcc
     2701 ctgccggaaa atgagccggg ttcttccatc atgccgggaa aggtgaatcc cactcaatgc
     2761 gaagccatga ccatggtctg tgcgcaggtt atcggaaatc acacgactat cacgatttcc
     2821 ggcgcacagg ggcatcttga actcaatgtg ttcaaacccg tcattgcgta caacgtgctt
     2881 caatccatcc gcctcctcgg cgatgccgtc atgagcctga ccgataaatg cattgtgggc
     2941 attgagccga atattgcccg tatttccgag ctgatggaac aatccctgat gctggtcacg
     3001 gcattggttc ctcacatcgg ctacgataag gctgccaaaa ttgctcaagg ggccttaaaa
     3061 aatggacgca ccctgcgaga agaagctatc gccagcggat ttgtgac
//
LOCUS       DAORAQ010000286         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4373_length_3095_cov_69.066447, whole genome shotgun sequence.
ACCESSION   DAORAQ010000286 DAORAQ010000000
VERSION     DAORAQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_3095_cov_69.066447"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKM72_14235"
     CDS             complement(<1..1007)
                     /locus_tag="PKM72_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNP62002.1"
                     /translation="MALHVALNHQTHYRYDRLVQMGPHVIRLRPAPHSRTPILSYALK
                     IEPTGYFLNWQQDPHGNYLARIIYPEKVGEFHVEVDLVADMVVYNPFDFFLEPDAETL
                     PFTYDPMVKEDLLPYLAKEPPGPRLQEWLRSFTAPHEDPTVPFLMSLNQQLKSQIDYV
                     VRMEPGVQTPDETLELGRGSCRDTGWLLVQILRHLGIASRFVSGYLIQLVADVKSLDG
                     PSGADHDFTDLHAWVEAYLPGAGWVGLDATSGLLAGEGHIPLACTPHPLTAAPISGVM
                     DICETTFSHEMSVTRIVETPRVTKPYTEEQWQAIDTFGQRLDQEMAANDVRLTIGGEP
                     T"
     gene            1291..2751
                     /locus_tag="PKM72_14240"
     CDS             1291..2751
                     /locus_tag="PKM72_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNP62003.1"
                     /translation="MKSLFDRYQMKSGFDEMFESSGVPRRHYRRLVERLRNFSSKDLE
                     LKRRQADQAFLRQGITFTVYGDLQQTEKIFPFDLMPRIIPDLEWRVIEEGIRQRVMAL
                     NMFLVDLYGDQRILNDQVVPRELIESSVHFQKDLVGFCPPKEVFIHVSGIDLIRNHEG
                     SYYVLEDNLRTPSGVSYVLENRLVMKQLFPNLFADMQVRPVDQYPTQLLENLKWLAPL
                     GKENPTVVLLTPGVFNSAYFEHCFLSLQMGIQMVEGRDLVVESDDKVYMKTTQGLEQV
                     DVIYRRVDDAFLDPEVFRKDSVLGVPGLVRAYRAGNVGLANGIGNGIADDKAVYTFVP
                     KMIDYYLSEKPILQNVPTYLCGNPEDRKYVLDHLESLVVKAVGESGGYGMLMGPSSTK
                     AEQEEFRKKIETDPRSYIAQPVVTLSHHPCIVDGEENMRLVGRHIDLRPFVLWGEEIS
                     LSLGGLTRVALKEGSLVVNSSQGGGSKDTWVLYGDE"
     gene            2755..>3095
                     /locus_tag="PKM72_14245"
     CDS             2755..>3095
                     /locus_tag="PKM72_14245"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNP62004.1"
                     /translation="MLSRTAESFFWIGRSVERAEYTARFVNVHFHLLTEIATREDQAN
                     IWTRYLEDTGELEAYTKIFGEVQTRPVMEFVTLCRDNPNSLTNLIAAARNSARGIQDQ
                     LSSEVWHFMND"
BASE COUNT          728 a          725 c          884 g          758 t
ORIGIN      
        1 aaggtgggtt ccccgccaat ggtgagccgg acatcattgg ccgccatttc ctgatccaac
       61 cgttgtccaa atgtatcgat ggcttgccat tgttcctcgg tgtagggctt ggtgacccgt
      121 ggcgtttcaa caatacgagt gaccgacatc tcatgggaaa atgtcgtttc gcaaatatcc
      181 ataaccccgc taatgggcgc ggcggtcagc ggatggggcg tgcaggcaag cggaatatgc
      241 ccttctccgg ccaacaggcc ggaggtggca tccagtccca cccacccggc tccaggcaga
      301 taggcctcga cccaggcgtg aaggtctgtg aagtcatgat cggcaccaga tggaccatcc
      361 aacgatttca catcggcgac aagctgaatc aaatagccgg agacaaatcg actggcaatt
      421 ccaaggtggc gaagaatctg cacgaggagc caaccggtgt cccggcacga tccacgacca
      481 agttcaagcg tctcatcagg ggtttgcacg ccgggttcca tgcggacgac gtagtcgatt
      541 tgagacttga gttgctgatt gagactcatg agaaagggaa cggtcggatc ttcgtgtggc
      601 gcggtgaatg atcgcagcca ttcctgcagt cgagggccag gtggttcttt ggccaaatac
      661 ggcagcaggt cttctttgac cataggatca taggtgaagg gaagtgtctc ggcgtcaggc
      721 tccagaaaaa aatcaaaggg gttatagacg accatgtcgg ccacaaggtc gacttccaca
      781 tgaaattccc caaccttttc ggggtaaata atccgtgcca gatagttgcc gtgaggatcc
      841 tgttgccagt tcagaaaata cccggtgggc tcaatcttga gtgcgtaact cagaatgggc
      901 gtccggctat ggggagccgg ccgcagccgg atgacgtgag gccccatctg aacgagccga
      961 tcgtagcgat aatgagtttg gtgattgagt gcgacgtgga gtgccatgaa gaattaagag
     1021 ccgatggaat acctgtgttg gtgataagga aagcggaaga atgcaatagc catcttcacg
     1081 tgaactgatt gaggaatctt agcaccctcc ctaatcgttt tgcatcatcc aacttgatga
     1141 gactgtgtaa tcaccaatcc aagacaagcg ttgcatgagc atttttctcc tgttgaaatt
     1201 gcccttgtgc cggccggcta tggtacactc ttttccgtaa attcggggga tcgttttttt
     1261 gccaagaggc cactgatcaa ggatagggtt gtgaaatcgt tatttgaccg ctatcagatg
     1321 aaatcaggtt tcgatgaaat gtttgagtcg tccggcgttc cgcgtcgtca ttatcggcgt
     1381 ctcgtcgaac ggttacggaa tttctcttcg aaagatctgg aattgaaacg gcgtcaggcc
     1441 gatcaggcct ttttgcggca ggggatcacc tttaccgtct atggcgatct ccaacaaacc
     1501 gagaagattt ttccttttga tctgatgcca cggatcatcc cggatctcga atggcgagtc
     1561 attgaagagg ggattcgcca acgcgtcatg gccttgaata tgtttttagt ggacttgtat
     1621 ggcgatcaac ggattctgaa cgatcaggtg gtgcctcgcg aactcataga aagttccgta
     1681 catttccaaa aagatctggt gggtttttgc ccgcccaaag aagtgttcat ccatgtatcg
     1741 gggatcgatt taattcggaa ccatgaaggc agctattacg tgcttgagga taatctccgg
     1801 acaccttcag gtgtgtcgta cgtgctggaa aaccgactgg tcatgaagca actgtttccc
     1861 aacctgtttg ccgacatgca agtccggccg gtggatcaat atccgacgca gctcctggag
     1921 aatctcaagt ggctggctcc ccttggcaag gaaaatccca ccgtcgtcct tctgactccc
     1981 ggtgtgttta actccgccta ctttgagcat tgctttttgt ctttacaaat gggaattcaa
     2041 atggtggaag gccgggatct tgtcgtggaa tctgatgata aggtgtatat gaagaccacc
     2101 caaggtctcg agcaggtgga tgtgatttat cgtcgggtgg atgatgcctt cctcgatccg
     2161 gaagtttttc gcaaggattc cgtgctaggt gttcccggcc tggtccgggc ctatcgggca
     2221 ggaaatgtgg gtttggccaa tggcattggc aacgggattg cggacgataa ggcggtctat
     2281 accttcgtac cgaagatgat cgactactat ttaagcgaga aaccgattct gcagaatgtc
     2341 ccgacctatt tgtgcgggaa tccggaagac cggaagtatg tgttggatca tctcgagtcg
     2401 ttggtcgtca aagccgtagg ggagtcaggc gggtatggga tgttgatggg acccagttcg
     2461 accaaagccg agcaggaaga gtttcggaag aaaatagaaa ccgatccacg cagctacatt
     2521 gcccagccgg tggtgaccct ctcgcaccat ccctgtatcg tggatggaga agagaacatg
     2581 cgattggtcg ggcgccatat cgatctccgg ccgtttgtgc tctgggggga ggaaatttcc
     2641 ttgtccttgg gagggttgac ccgtgtggcc ttaaaggaag gctccctggt ggtgaattct
     2701 tcccaaggcg gaggaagtaa ggacacgtgg gttctctatg gggatgaata gaccatgttg
     2761 agtcgaacgg cagaatcgtt tttctggatc gggcgctcgg tggagcgggc tgaatatacc
     2821 gccaggttcg tgaatgtgca ttttcatttg ctcaccgaga tcgcaactcg agaagaccag
     2881 gccaatatct ggactcgata tttagaggat accggggaac ttgaggccta taccaagatt
     2941 tttggggaag tgcagactcg accggtcatg gaatttgtga ccctctgccg tgacaatccg
     3001 aattcgctga ccaatctgat tgcagctgcg agaaatagcg cccgtggaat ccaggatcag
     3061 ttgtccagcg aagtctggca ttttatgaat gattt
//
LOCUS       DAORAQ010000287         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4396_length_3085_cov_61.873267, whole genome shotgun sequence.
ACCESSION   DAORAQ010000287 DAORAQ010000000
VERSION     DAORAQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4396_length_3085_cov_61.873267"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKM72_14250"
     CDS             <1..272
                     /locus_tag="PKM72_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNP62005.1"
                     /translation="LSAGFITLPGGFGTLEECCEMVTWAQLQLHHKPCGLLNCFGFFD
                     GLLRFFDHQVQEGFLIQANRELLLEASTPEDLLTLVLSRLEPDIR"
     gene            complement(284..1093)
                     /locus_tag="PKM72_14255"
     CDS             complement(284..1093)
                     /locus_tag="PKM72_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62006.1"
                     /translation="MKPFLPNLLSDQRVVDKQTTPTGRARVFVLAMSCLVMTASVGYS
                     EEQPHELPKPFHKTTPPLFQEWKFDQSALSDLPEGFTKAVGEPSGKGEWSVKEDPSAP
                     SRPHLVEQRLHCAEDWCYEILIAEGAVAEYVDLSVQIKLNLGTPSGKAGLAYGVQDNK
                     NFYAAVVEPSTNEVVVYVVKNGAPTELGRESLIPRSAEWHFLRIQRNTIISKEITEIS
                     FDNHLVLSVADQSFKKGQLGIVAIGDGSFSFDNLRAMELQSNRPLSRPSAY"
     gene            2238..2834
                     /locus_tag="PKM72_14260"
     CDS             2238..2834
                     /locus_tag="PKM72_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNP62007.1"
                     /translation="MNMRGFWLGLVVGTTFTFLLGQWAFPAASKETDPPNGLSPAIVA
                     DYVHSVVQADRTFYQMEIVDRMQEKGLAFASENWKMDGDLPLPAQFLQESGRLMAEQQ
                     KLIQFRLISSWAINKEHRPTTVFQRTGLTKILENPDRPYTGVTTEGKARIFQALYADK
                     AVSQHCADCHNGHPQSLKRDFKAGDVMGGVLVTIALPQ"
BASE COUNT          841 a          784 c          697 g          763 t
ORIGIN      
        1 atctgtcagc cggattcatc acgttacccg gtggatttgg aacattggaa gaatgttgcg
       61 aaatggtgac ctgggcacag ttacaacttc accacaaacc ctgtggactg ctgaattgct
      121 ttggtttctt tgatggtctc ttgagatttt ttgaccatca agtccaagaa gggtttctca
      181 tccaggcaaa cagggagtta ctccttgaag cctccactcc tgaagacttg ttgacccttg
      241 tcttaagccg actcgagcct gatatccgct aatagggtgc cgctcaatag gctgatggcc
      301 tggaaagcgg tcgattcgac tggagttcca tcgcccgaag attatcaaac gaaaaactcc
      361 cgtccccaat tgccactatt cccagctgac cctttttgaa tgattgatca gccacgctca
      421 gcaccaaatg gttatcaaaa gagatttcgg taatctcctt gctgataatc gtattccgtt
      481 gaattcggag aaagtgccat tcagcagatc gaggaatcag ggactccctg cctaattctg
      541 ttggtgctcc attcttgact acataaacca caacctcgtt agtgctaggt tcaacaaccg
      601 cggcgtagaa gttcttatta tcttggaccc cataggccaa tccagctttt cctgacggag
      661 ttcccaaatt aagtttaatc tggactgaga ggtccacata ttcggccacc gcgccttctg
      721 cgatcaatat ctcgtagcac caatcttcag cacaatgtaa gcgctgttcc accagatggg
      781 gacgactggg ggcagaggga tcttctttga ctgaccattc cccttttcct gaaggttccc
      841 cgaccgcctt ggtaaagccc tcgggtaagt cacttaaagc cgattgatca aatttccatt
      901 cctgaaaaag agggggagtc gttttatgaa atggcttagg aagctcatga ggttgttctt
      961 cggaatatcc cacggaagcc gtcatcacaa ggcaactcat ggccaacaca aagaccctcg
     1021 ctcgaccggt aggagtcgtt tgtttgtcca ccaccctttg atcagacaag agatttggca
     1081 gaaaaggctt catagacgga ctcagctcct catgtttcat aaccatcctt accctcaaat
     1141 gatatgaata ttaaatagac aaaaaatcca tcgcgcctag gatgatacgc attctcaccg
     1201 gaagtccaca acccctgaat ggcaacccac atccatccca atcacggcat caaaatccct
     1261 cctccctcga tcctcccata gagcagtctt tgctcaggtc ttctgaggaa aattaatcat
     1321 cccattcact cgcggcattg ctatagaaaa gaggctatct catctgacca ggtagccaag
     1381 gcgttctctg tccaactatt cccctgaaaa attgcagaaa tatacggctt tgataaattc
     1441 aggatcaagc agatcgtgga aacattaaac aggcatacgt tggatcatgc catcttaagc
     1501 tttgatccga cttccttttg taaccgacct cactctcaag aaactggcct agccgagcat
     1561 cagtgtgagg agtccccgca ttctttctga gagtgaaaat tatgtatttc ggaatcacta
     1621 aataagactg aatttcttca caccataaga ggaggaaatt gatatctgga attcaaatac
     1681 actattcccc ccacaccaac ctccaagtgg tcttggaagc ctgccatcat ggattggtat
     1741 agtcggcgca tgcatttgtt aatggcgaaa acccattcaa gtcggcatag atggcctcgg
     1801 acgggtgttc aataacatct ttttcaaacg actctggcgg aaggggttgt acacaaaagt
     1861 ctatctgagt gtgagtgact ttcagagcat gcagaaaact tatgctgatc aaggcctatt
     1921 ttgaatttca ttattacgaa aggttacact aaacagccta tagaacgttg gcgcaggtat
     1981 acgcggtcac ttagaccaaa agagcccggc cagacagagc aagagcctcg gacaggagga
     2041 ttgggataag aatacgaaca agtcagtaaa gggagcaata actccagaaa tacccatttt
     2101 aaaaagcgct ggttttggtc ccgacaatgg gagcaccata gttcaaaaac tgttcggact
     2161 tacttgtttg agagcaccgc tatcggagcc aatgagcgaa tcaagccaca aacagaaaaa
     2221 gaaatggaga acgcatcatg aacatgagag gcttttggtt gggcctcgtg gttggaacca
     2281 ccttcacgtt cctactcggt cagtgggcgt tccccgccgc gagcaaggag acagatccgc
     2341 ctaacggcct atcgcccgca atcgtggccg attatgtgca tagtgtagtc caagcggatc
     2401 gaacctttta ccagatggag attgtggatc ggatgcagga gaaaggcctt gcctttgcct
     2461 cggagaactg gaaaatggat ggagacctcc cattacccgc ccaattcctg caggaatcgg
     2521 ggcgactcat ggcagagcag caaaaactga ttcaattccg gttgatcagc agttgggcga
     2581 taaataaaga gcatcgccca accacagtgt tccagcggac cgggcttacg aagatccttg
     2641 agaatcccga tcgtccctat acaggcgtta caaccgaggg caaggctcga atcttccaag
     2701 cactctatgc cgataaagcc gtatctcagc actgcgcaga ttgtcacaat ggacatcccc
     2761 aaagcctcaa gagggatttc aaggcgggag acgtgatggg tggggttctt gtgacaattg
     2821 cactccccca atagagaatg ccgcctgaag gtaagcagct ttcatcgccc ttgagtgtgg
     2881 gataacctgg ctgttgtggg ctgcttgagt ctgattaggc tgaaaaagga ccatgaaacc
     2941 ggttttccaa tcgcttcgag gggattgtag aagggcttga cctaccaagc tatccctcac
     3001 ccctgcataa acactactga atctctttat tcccctactc cacggtttac ctctcttcac
     3061 cccaatttgt cactttttct cacac
//
LOCUS       DAORAQ010000288         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4398_length_3084_cov_62.973919, whole genome shotgun sequence.
ACCESSION   DAORAQ010000288 DAORAQ010000000
VERSION     DAORAQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_3084_cov_62.973919"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1628)
                     /gene="cobA"
                     /locus_tag="PKM72_14265"
     CDS             complement(57..1628)
                     /gene="cobA"
                     /locus_tag="PKM72_14265"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNP62008.1"
                     /translation="MTKSVPNHGRVFLVGAGPGDAKLLTLRGRECLEQADVVLYDHLA
                     NPELLKFAPSHAECIYVGRKGRGVYRDQAEIHVLLVSKAQEGKCVVRLKGGDPFVFGR
                     GGEEAEVVADAGIPFEVVPGVTAAVAVPAYAGIPVTHRTLASTVAFVTGHEDPTKPSS
                     AMEWPRLASAEGTLVFLMGMKNLPQIVARLIQEGKPATTPVAVIRWGTYARQRTVVGT
                     LSDIVGLASRADMSPPSVIVVGEVVRLRERLNWHESRPLFGQGILVTRPRAQAPALSN
                     LLAGQGAEPVECPTLEIHPPDSWSPVDEAIAAISTYDWVIFTSVNGVQSFIKRLWFHH
                     KDVRSLAGIRVCCIGPRTREEAARWGIASDLVPQDFQAEGILQALGGLGVSGQRVLIP
                     RAKVAREILPDQLEAMGATVHVVHAYQALPPSVDIGLLRDRLRNREIQYVTFTSSSTV
                     RNFCQLFENRQELQELTRHLIVAVIGPITAQTVQEEGLSVDVMALESTVPALVDAIII
                     HAQQAPKDTRLVSSS"
     gene            complement(1625..2575)
                     /gene="hemC"
                     /locus_tag="PKM72_14270"
     CDS             complement(1625..2575)
                     /gene="hemC"
                     /locus_tag="PKM72_14270"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNP62009.1"
                     /translation="MNQTDSRARSTLIVGTRGSQLAIWQAEWVQRQLKALAPDISVIL
                     KRIQTSGDKIQDVPLAKIGGKGLFVKEIEEALLRQDIDLAVHSMKDVPSELPEGLEIV
                     CVPEREDPRDALIAHEGHILATLPVGARVGTSSLRRQAQLLHARPDLEIAMLRGNVDT
                     RLRKVREGHYDAIILAASGLKRLGWDQEVTEYLSVDVSVPAIGQGSLGLEGRKDDIWV
                     RDLVGQFEHRSTRIAVTAERALLAGLEGGCQVPIAGYATIHGDTLTLVGLVASLDGKR
                     YIRQVLSGPNGEAASIGTRVAEQLLDGGAKPILQEIYGMA"
     gene            complement(2572..>3084)
                     /locus_tag="PKM72_14275"
     CDS             complement(2572..>3084)
                     /locus_tag="PKM72_14275"
                     /inference="COORDINATES: protein motif:HMM:NF012947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNP62010.1"
                     /translation="PRNIDPAVKDVDNAYVFDIDDLKAHVGHNQEERLKEAETAESMV
                     REEVGSMVAWVRGLEATPTIVALRKKAEEIKRGELEKVFGRLGELSPKERAAIEGLAS
                     AIVNKLLHGPVVTLKTEAQSQNGFAFIEAARRFFELREREIHSEEVQLLEDETGDGVS
                     GEPLSQKDGM"
BASE COUNT          774 a          972 c          646 g          692 t
ORIGIN      
        1 tcccatcaat cggttaacaa aacgtggtgt ctgaaacttc ctccaccata gaataattat
       61 gaagacgaga ccaatcgcgt atccttcgga gcttgctggg catgaatgat tatggcatcg
      121 accaacgccg gaaccgtact ctccaacgcc atgacatcca ccgacaaccc ttcttcctga
      181 acggtttgag ctgtgatcgg gccaatgacc gccacgatca aatgccgggt taattcctgc
      241 aattcttggc gattctcaaa caactgacaa aaattcctca ccgtggaaga actggtaaag
      301 gtcacatatt gaatttccct attgcgaagg cgatcgcgaa gaaggccaat atcgaccgaa
      361 ggagggagcg cttgatacgc atgaaccaca tgaaccgtgg cacccatggc ttccaactgg
      421 tcagggagaa tctcgcgtgc cactttggct cgaggaatca aaacccgttg accggagacc
      481 cccaacccgc cgagcgcctg caaaattcct tctgcctgaa aatcctgagg aaccagatcc
      541 gatgcaatcc cccaacgagc agcctcttcc cgcgtccgag gaccaataca acacacacgg
      601 attccagcca aactccttac atctttatga tgaaaccaga gacgttttat aaatgattgc
      661 acaccattca cactcgtaaa gatcacccaa tcgtatgtcg aaatggcagc aatcgcctca
      721 tccacaggag accaactatc aggaggatga atctccaacg ttggacattc caccggttcc
      781 gccccctgcc ccgccaacaa gtttgaaaga gccggggcct gggcacgagg tctggtcact
      841 aagatgcctt gtccaaacag aggccttgat tcatgccagt tcagtcgttc acgcaaccga
      901 acgacttcac ccacaacaat cacggatggc ggactcatat ctgcccgaga ggctaatccc
      961 acaatgtcgg ataaggtacc caccaccgtt cgttgacgag cataggtccc ccacctgatc
     1021 accgccacgg gagtcgtcgc aggcttgcct tcttgaatga gccgggccac aatctgcgga
     1081 agatttttca tgcccattaa aaataccagg gtcccttcag ccgaagcgag acgcggccat
     1141 tccatcgcac tcgatggttt cgtcggatct tcatggccgg tcacaaatgc gaccgtcgag
     1201 gcgagagtcc ggtgcgtcac gggaatacca gcataagccg gcacagccac ggcagcagtc
     1261 actccaggca caacttcgaa tgggatccct gcgtcagcaa ccacttcggc ttcttcacct
     1321 ccacggccaa acacaaacgg atccccaccc ttgagtcgca ccacacactt accttcttga
     1381 gccttactca ccaacaagac atgaatctct gcctgatctc ggtagacccc acgccctttt
     1441 cgcccgacat aaatacactc ggcatgagaa ggggcaaatt tcaacaactc cggattagcc
     1501 aggtgatcgt agagcaccac atccgcctgc tctaaacatt ccctcccccg taaggtcagc
     1561 aacttggcat ctccgggccc agctcctact aaaaaaaccc gaccatgatt tggaactgat
     1621 ttggtcatgc catcccataa atttcctgga gaatgggctt cgccccccca tccaacagtt
     1681 gttcagccac cctcgtgcca attgaagcag cctccccatt tggccccgat agcacctgac
     1741 ggatatatcg tttcccatca agactggcga ccaaccccac aagagtcaac gtgtccccgt
     1801 ggatagtcgc ataacctgca attggaacct gacatccgcc ttcaagacct gcaagcaatg
     1861 ctcgttcagc agtaaccgca atcctggtag accgatgttc aaactgccca accagatctc
     1921 gaacccagat atcatctttt cgtccttcca gccctaacga accttgccca attgccggca
     1981 cactcacatc cacggacaaa tactccgtca cctcttgatc ccaacccaag cgcttgaggc
     2041 ctgaggcggc cagaataatg gcatcataat gaccttcgcg aaccttgcga agtcgggtat
     2101 caacattccc acgcaacatc gcaatctcaa gatccggacg cgcatgtaac aactgcgcct
     2161 gtcttcgcaa actactggtt ccgactcgtg caccaaccgg caatgtggct aaaatatggc
     2221 cttcatgcgc aatcaacgca tctctgggat cctctcgctc gggaacacaa acaatctcca
     2281 gtccttcagg taactctgaa ggcacgtctt tcatgctatg caccgctaga tcaatatctt
     2341 gtctcagcag ggcttcttct atttctttaa caaacagccc ctttcctcca attttggcca
     2401 aaggtacatc ttgaatttta tccccactgg tttggatgcg cttcagaatg acagaaatat
     2461 ccggagcaag ggctttcagc tggcgttgca cccattcagc ttgccatatg gctaattggc
     2521 ttcctcgtgt tcccacgatg agcgtcgatc gtgctcgact atccgtctga ttcacatgcc
     2581 atccttttgc gacaggggct ccccactgac gccatcaccc gtctcatctt ctagaagctg
     2641 gacctcttca gagtgaatct cccgttcccg cagttcaaaa aaccggcgag cggcttcaat
     2701 aaacgcaaat ccattttgag attgcgcttc ggtttttaag gtcacgaccg gaccatgcag
     2761 taatttattc acaattgcgg aggccagccc ttcaatggca gcccgctcct tcggcgacaa
     2821 ttcccccaaa cgaccgaaga ctttctccaa ctccccccgc ttgatctctt ccgctttttt
     2881 tctcagggcc actatggtgg gggttgcctc cagtccgcga acccaggcca ccatgctgcc
     2941 gacttcttct ctcaccatgg actcggcggt ttcggcttct ttcaatcgtt cttcctgatt
     3001 atgccccaca tgggccttga gatcatcaat gtcaaacaca taggcgttat caacatcctt
     3061 gaccgccgga tcaatatttc gagg
//
LOCUS       DAORAQ010000289         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4418_length_3077_cov_63.444739, whole genome shotgun sequence.
ACCESSION   DAORAQ010000289 DAORAQ010000000
VERSION     DAORAQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_3077_cov_63.444739"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PKM72_14280"
     CDS             <1..1075
                     /locus_tag="PKM72_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNP62011.1"
                     /translation="SWAGEVVRMKNRVQSSRLSKPWGFFILSSCVRQVICGIVAWWVI
                     FPCGFPGVMVLAFDGEMMKRRQQGILTGMPFMANVAPRTFVDDLGRKLYLANPPTRIV
                     SLAPSVTEILFAIGLNEEIVGVTEFCDFPPEALAKPKVGYATPNLETILGLQPQLVLA
                     PRSFLRADLITKLEQLKIPIFILEAHTVEDILAHIQLLGRMVGHSEEANAQAALMRKQ
                     LASLSSRLVDLPRPTLLYILNSSPLITVGPGSFIHRLIELAGGRNAAEQAVAPYPRLT
                     MEEVLRQNPDILLFPVGRQEGISQVEQDSWKRWTTLAAVQHGKLVQVDSDLLNRPGPR
                     ILEGLKYLVKILHPEVSQDNLPN"
     gene            1193..2335
                     /locus_tag="PKM72_14285"
     CDS             1193..2335
                     /locus_tag="PKM72_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNP62012.1"
                     /translation="MAKVPPLTISPSTTGSKEWVHSPPTMPMPVPSLPPLTIRRWCVW
                     VGISIMLAVAALWACLGFGTESIAFSRIWSILMGALMGRESVMIGTDPASVILLQVRL
                     PRLILAFFVGGSLAMVGVALQALLRNPLADPFIIGISSGAALGAAIAILFGVGISVWS
                     VSALPVCAFAGAVLSLLIMYRISSAGTGFSIYTLLLAGVVLNAIFSAFIMFLTSVADP
                     NRAFGMYAWLMGSLTGPDFSTLGVLALYLGVGLFVLGTQAQSLNLLTLGEETARSLGV
                     EVERVKQMVFVASALLTGAVVSFSGIIGFVGLIVPHAVRLVLCADHRLLLPVAGFAGG
                     IFLMFADTIARTLLSPAEFPVGVVTALVGGPVFLFLLMNRNMRVGM"
     gene            2335..>3077
                     /locus_tag="PKM72_14290"
     CDS             2335..>3077
                     /locus_tag="PKM72_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP62013.1"
                     /translation="MLNPDSFIAAPAYALRDVTFRYATSSRDTSESVLKNLTCSIDAG
                     KILGILGPNGSGKSTLLKLLARVLLQGSGTIELFGNSFSLLSQAEVARRVALVPQETM
                     QIFPFTIAEMVLMGRFPHHRGWGGWHWEDSDDWRIAHQAMEELDVTHLGSRLVTDVSG
                     GERQRAVIARALTQEPQILLLDEPTAFLDLHHQLDIARILRRLNRERGLTVILVSHDL
                     NLASQYCDRLMLLHHGQIVEVGSPLEVLRP"
BASE COUNT          587 a          724 c          896 g          870 t
ORIGIN      
        1 ctcgtgggct ggggaagtgg tgaggatgaa gaatcgggtg caatcctcaa ggttgagtaa
       61 gccttgggga ttttttattt tgagcagttg tgttcgccag gtgatctgcg gaatcgtggc
      121 ttggtgggtg atcttcccat gcggatttcc tggcgtgatg gttttggcct ttgacggaga
      181 gatgatgaaa cggcgccaac agggtatttt gaccggcatg cccttcatgg ctaatgtggc
      241 tccccgcacc tttgtggacg atcttggacg gaaactctat ctggccaatc cccctacgcg
      301 cattgtttca ttggctccta gtgtgaccga aatccttttt gccatcggat tgaatgagga
      361 aattgtcggg gtcaccgaat tttgcgattt ccctcccgag gcattggcta agccgaaggt
      421 cggatatgcc acccctaatc tggagacgat tctgggactt caaccgcaat tggtgttggc
      481 cccaaggtct ttcctgcgtg ccgatctcat caccaaattg gaacaattaa agattcctat
      541 ctttatcctt gaggcgcata cggtggaaga cattcttgcg cacattcagt tgttggggcg
      601 gatggtcggt cattctgaag aagcgaatgc ccaagcggcc cttatgcgga aacaactcgc
      661 cagtttgtcg agccggttgg tggatctgcc tcgccccaca ttattgtata ttttgaattc
      721 ctcaccactg attacggtcg ggcccgggag ttttattcat cgactgattg agctggcggg
      781 tggcagaaat gctgctgagc aagctgtggc gccttatccg cgtctgacca tggaagaagt
      841 cttacgacaa aatcctgata ttctgttgtt tccggtgggg cgacaggaag ggatttctca
      901 ggtggaacag gattcatgga aacggtggac gacattggca gcggtgcaac acggcaagct
      961 ggttcaagtt gatagcgatc ttctcaatcg tcccggtcca cggattcttg aagggctgaa
     1021 gtatttggtg aaaattctgc atccggaagt gagtcaggat aaccttccaa attgagtgat
     1081 cttgtgaaga atcatcgaga tccgggaatg ggaaatacct ttccggtcgg atccggaaca
     1141 tttcaccatt gcgctcactg aatccagaaa ataaaccttg ttggtggacc ccatggcaaa
     1201 agttccgcca ctgaccattt ccccatcgac aacaggttcg aaggaatggg tacattctcc
     1261 tcccactatg ccaatgccgg tcccctctct accgccactc acgatccgtc ggtggtgtgt
     1321 gtgggtgggc atatcgatca tgttggcggt agcagctctg tgggcctgtc ttggatttgg
     1381 gaccgagtct attgcgttct cccggatctg gtccattctg atgggggcat tgatgggaag
     1441 agagtccgtg atgatcggga cggatccggc atcagtcatt ctgcttcagg tgcgtttgcc
     1501 tcgcctgata ttggcatttt tcgtaggtgg gagtctggcg atggttggcg tcgcacttca
     1561 ggccctgttg cgcaatcccc tggctgatcc gtttatcatc ggtatttcca gtggagcggc
     1621 gctgggcgcc gctattgcca ttttgtttgg cgtgggaata tccgtgtgga gtgtgtccgc
     1681 tttgcccgtg tgcgcttttg cgggagccgt tctgtccttg ttgattatgt atcgcatttc
     1741 atcagcgggc actggttttt cgatttacac cctgttactc gccggggtgg tgttgaatgc
     1801 gattttttcc gcattcatta tgtttttgac cagtgtggca gatcccaatc gggccttcgg
     1861 gatgtatgcc tggttgatgg gatcattaac cggcccggat ttctccaccc ttggcgttct
     1921 ggctctatat ttgggagtag gattatttgt tttggggaca caagcccagt cattaaatct
     1981 cttgaccctt ggcgaagaaa cggcccgatc gttgggagtt gaagttgagc gggtcaaaca
     2041 aatggtattt gtcgcatcag ctctgttgac aggggcggtc gtgagtttta gtggcattat
     2101 cggatttgtc gggctgatcg tgccgcatgc tgtccgtctg gtcttgtgtg cggatcatcg
     2161 actcttactc ccggttgccg gatttgcggg aggcattttt ctgatgttcg ccgatacgat
     2221 cgcgcgaacc ctcttgagtc ccgctgaatt tcctgtcggt gtcgtcacgg cgttggtggg
     2281 cggccccgta ttcctgtttc tgctgatgaa tcggaatatg cgggtgggga tgtgatgctg
     2341 aacccggatt cttttattgc cgcgccggcc tatgcgcttc gagatgtgac ctttcgctat
     2401 gccacgtctt cccgcgacac atctgaatcg gtgctgaaga acctcacctg ctcgattgat
     2461 gccgggaaga tccttgggat tcttggaccg aatggatcag gcaaaagtac cttactcaag
     2521 cttctagcgc gtgtcctgct tcaggggtcc ggtacgattg agttgttcgg gaattcattt
     2581 tccctactgt ctcaggctga ggtggccagg cgggtggcgt tggttccgca ggagaccatg
     2641 cagatttttc cctttaccat cgcggaaatg gtgttgatgg gacgatttcc tcaccatcgc
     2701 ggttggggtg gctggcattg ggaggattct gacgactggc gaatcgcaca tcaggctatg
     2761 gaagagctgg atgtcacaca tctggggtcc agactggtca cggatgtgtc gggaggggaa
     2821 cgtcagcggg cagtgattgc gcgagccctt actcaggagc cacaaatatt gctcttggat
     2881 gagcctaccg cttttttgga tttgcatcat cagttggata ttgcgcgaat tcttcggcga
     2941 ctgaataggg agcgtggcct cacggtgatt ctggtgtctc acgatttaaa tctggccagt
     3001 caatactgtg accgcctgat gttgttacat catggacaga ttgtggaggt cggatcgcct
     3061 ttagaagtcc tgcgtcc
//
LOCUS       DAORAQ010000290         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4494_length_3034_cov_69.194696, whole genome shotgun sequence.
ACCESSION   DAORAQ010000290 DAORAQ010000000
VERSION     DAORAQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4494_length_3034_cov_69.194696"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            802..2241
                     /locus_tag="PKM72_14295"
     CDS             802..2241
                     /locus_tag="PKM72_14295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNP62014.1"
                     /translation="MHFRRQILIKLMHFGDLLLLGMSFLIVLWVFYAPNNSLMAFEEF
                     LALRIKIENFLVGAVMVGIWHVGCASLGLYESKRLSTIAQEVPDIIKATLLVTFVTAG
                     IALLFRIDMVSAEVLLSFWILSTVLICLSRVGVRVTLAWMRRKGRNLRNILIIGTNPR
                     ALAFAKEIQSKPELGYCLLGYVDEPWDGLNHEHLEDVPILTDLKKFPGFIRTFSVDEV
                     LLALPFRSFYQEAAQVVAQCEEQGILVRVLGDLFSPKLAKSTIDEVGLHPTFSLNTGA
                     IGDQAVLIKRAIDLIIAIPSVILLLPFFAIVAAAIKLSSPGPVFFIQERVGLNKRLFP
                     FIKFRTMVPDAELQQAELEHLNEVDGAAFKITNDPRVTPLGRLLRKASIDELPQLINV
                     INGDMSLVGPRPLPVRDLQEFYEDWHRRRFSVRPGLTGLWQVSGRSSTSFAKWMELDL
                     HYIDKWSIWLDLKILFKTIPAVMKGSGAV"
     gene            2277..>3034
                     /gene="gmd"
                     /locus_tag="PKM72_14300"
     CDS             2277..>3034
                     /gene="gmd"
                     /locus_tag="PKM72_14300"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNP62015.1"
                     /translation="MSKTALITGITGQDGSYLAELLLSKGYSVHGIIRRASTFNTARI
                     DHIYADPHDPHTKLFLHYGDLTDSSQLTNLIYNTQPQEVYHLGAQSHVRVSFDTPEYT
                     GDVTGLGTTRILEAIKRSEVSCKFYQASSSEMFGNASPPQSEDTAFYPRSPYGAAKVY
                     AYWMTVNYREGTNLFACNGILFNHESPRRGETFVTRKISRAVAHIKAGKQKDLYMGNL
                     EAKRDWGFAPEYAEGMHHMLQMDTPCDFVLGTGET"
BASE COUNT          772 a          644 c          681 g          937 t
ORIGIN      
        1 aatggcagtc aggtgtatcg cgtgttcaat ccttccttgt ttctctgagt aggctgaaag
       61 ccacctcacg gacgtttcta tcccgcacac acgtttgggt atatccgctc cttgataaat
      121 tatccgcttg ctggaaggcg ttcctttata tatgctgttt tctgatggtg gatggttttc
      181 aaagtgggat tgagttttcg atgtagggat aagcctacgt tgctgaaata ttgaggacga
      241 tggttgaaaa cgttggaaac actaggaata tggcggaagt taagcgcatg gcgggccgag
      301 attgcgtagt attactgttt ctttattaat acatacttct tgtgctatta aaaaaaattc
      361 ttggaacgaa tcgccaaaca gtgggtatta aactggaatg gcctgcatta ctccctgatt
      421 tcctcttagg tacatatttt gctttatata atattcattc attaaatttt taagaaatgg
      481 ttgctttgca caaatcgatt tgtgtatgtt tgcttagaat ttgcacttaa ttatttaata
      541 ttacgcatca ctatttatat tttgtgtttt aaaaagtgcg atggatttta ttttaatttc
      601 atttaattct cctcatacca attttttcat ctccccactt ttcatttccc cctattcatt
      661 ttcatccatt ttttatcgtg atgatgtatt acacgatgtt gcaacgtaat actcccggca
      721 tgtattcgtt cagattctga attaggttca ttcaattagg gcagggtatt cgactgtttt
      781 aagtaacggg cgggtttgtg tatgcatttt cgaagacaaa ttctaattaa attaatgcat
      841 ttcggtgacc tgctgttact cggcatgtct tttctgattg ttttgtgggt gttctatgca
      901 ccgaacaaca gcctaatggc ctttgaggaa tttttggcat tgcgaattaa aattgaaaat
      961 tttctcgtag gggctgtcat ggtgggaatc tggcatgtgg ggtgtgcctc cttggggctg
     1021 tatgaatcga aacgactgtc tacgatcgcc caagaggtac cggatatcat taaagccact
     1081 ttgctcgtga ccttcgtcac ggcagggatc gctctcctat ttcgaatcga tatggtgtcc
     1141 gccgaagtac ttctaagctt ttggatcctt tcgactgtac tgatatgtct cagccgggta
     1201 ggtgtgcgag taacgttggc atggatgaga cgaaagggaa gaaatttacg caatattctc
     1261 atcattggca ccaatccacg tgcattagca ttcgctaaag aaattcaatc caagcctgaa
     1321 ttgggatact gcctactagg atatgtggat gagccgtggg atggactcaa tcatgagcac
     1381 cttgaggatg ttcctattct aactgatctc aaaaaattcc ctggatttat tcgaacgttt
     1441 tcggttgatg aagtgctcct cgctcttcct tttcgctctt tttatcaaga agctgctcaa
     1501 gtcgtggcac aatgtgaaga gcagggtatt ctcgttcgtg tgttgggaga tcttttttct
     1561 ccgaagctgg caaagtctac cattgatgag gtgggactcc atcccacttt cagcctcaat
     1621 accggagcaa ttggagatca ggctgttttg attaaacgag ctattgattt aattattgcc
     1681 atcccttcgg ttattctcct tctcccattt tttgcaatcg tcgctgcggc cataaaattg
     1741 agttcacccg gaccggtctt tttcatacag gaacgagtcg ggttgaataa acggttattt
     1801 ccttttatta aatttcggac catggtgccc gatgctgaat tacagcaggc ggaactcgaa
     1861 catttaaacg aagtcgatgg agctgcattt aaaattacca acgatcctcg ggtgacacca
     1921 ttagggcgct tgttgcgcaa agccagtatt gatgaattac cgcaattaat aaatgtcatc
     1981 aatggagaca tgagcctggt tggcccacga ccgcttccgg ttcgagatct tcaggagttc
     2041 tatgaggatt ggcatcgtcg acgctttagc gtcaggccag gtctcaccgg tctatggcag
     2101 gtcagtgggc gaagttctac ctcctttgcg aaatggatgg aacttgacct gcactatatc
     2161 gataagtggt caatctggtt ggatctaaaa atcttgttca agaccattcc ggctgtaatg
     2221 aaaggaagtg gggcggttta agatctcttt tttaaatttc cccccaaact taatatatgt
     2281 caaaaactgc gcttattacc ggtatcaccg gacaagatgg gtcttatctg gccgagcttc
     2341 ttctttccaa gggatactcc gttcatggaa taatcagacg ggccagcacc tttaatactg
     2401 ctcgcattga tcacatctac gccgaccctc atgatcccca taccaagctt ttccttcatt
     2461 atggcgatct taccgattca agccaactga ccaatctcat ctataatacg caacctcaag
     2521 aggtctatca tctcggagcg caaagccatg tccgggtgag ttttgatact ccagaatata
     2581 ccggagatgt gacaggccta ggcaccaccc gtatccttga ggctataaag cgaagcgagg
     2641 tatcctgcaa attttatcaa gcttcctcga gcgaaatgtt tggaaatgcc agcccccctc
     2701 aatcggagga taccgccttt tatcccagaa gcccctatgg agcggcaaag gtctatgcct
     2761 attggatgac agtcaattac cgggaaggga ctaatttatt tgcctgcaat gggattcttt
     2821 ttaaccatga atcccccaga cgaggagaaa cctttgtcac gcggaaaatc agccgcgcag
     2881 ttgctcatat aaaagctgga aagcaaaaag atttgtatat gggaaatctg gaagctaaaa
     2941 gggactgggg ttttgcgcca gagtacgcag agggcatgca ccatatgctt caaatggata
     3001 caccgtgtga ttttgttctg ggtactggag aaac
//
LOCUS       DAORAQ010000291         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4535_length_3014_cov_67.533964, whole genome shotgun sequence.
ACCESSION   DAORAQ010000291 DAORAQ010000000
VERSION     DAORAQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4535_length_3014_cov_67.533964"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKM72_14305"
     CDS             <1..194
                     /locus_tag="PKM72_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62016.1"
                     /translation="LYVSDHKLPAFHLKRLFFLKKDLQPSNEGIQRNQLEKQLLRIFP
                     YSLLFIGSGKAETLFHLIY"
     gene            239..685
                     /locus_tag="PKM72_14310"
     CDS             239..685
                     /locus_tag="PKM72_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62017.1"
                     /translation="MNRTSQRRMIQEGIIQTVSRMMGLFMLTIALSLSGAAAWADGDG
                     HGYSHDPKSKLEKLTKKLDLTKEQQDKILPIIEEKHQKMEGLHQQMKDMRQQAMAKIE
                     AELTPEQKEKWKEMQEKRAEKMKEYKEKHGKECDKKGKHGKDEDDD"
     gene            1132..1449
                     /locus_tag="PKM72_14315"
     CDS             1132..1449
                     /locus_tag="PKM72_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62018.1"
                     /translation="MLEMVEIRVSYRYVKEHSWVVQGITGFLSAYFMEKPGFALKRHF
                     EELETGMHVWLCDIPPKMKVPTLLRRLKDDIPPCQYKQLETSPPARPQFLIDSLESET
                     ATS"
     gene            complement(1559..1999)
                     /locus_tag="PKM72_14320"
     CDS             complement(1559..1999)
                     /locus_tag="PKM72_14320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNP62019.1"
                     /translation="MTEPSCKICVGAWPLKDHFLVDCGLTRAYVFEDQFFAGWTVLVL
                     KEHVSELFDLSRVARSMLMEEVSHVAQTLAQVFDAKKINYELLGNQVPHMHWHLIPRL
                     KTDPDPLKPVWCVPHEPVGLPAEQLAERIHLLRSALCIDPTQSL"
     gene            2308..>3014
                     /locus_tag="PKM72_14325"
     CDS             2308..>3014
                     /locus_tag="PKM72_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62020.1"
                     /translation="MTIETHRLSRRTLLKRGMGIFGLLAGGFSGASSLAEILKEPTPR
                     QTLGPFFPDEGDPIDAIRENHAIGIPISQANDQDLTFVKGRRGKAKGQVIYLKGKVLS
                     AKSGKPIPHTSIIMWSASASGRYNHKEDGGMLKFPHPTTGEIIHRTYDAYFQYWGQVV
                     TNEQGDYWFKTIVPGFYPIDLQAGLYRPSHLHFQLVPPGHPKLVTQLYFRGDQIPNNE
                     LNQKLLPMDVVILDAGLT"
BASE COUNT          902 a          766 c          685 g          661 t
ORIGIN      
        1 ccctgtatgt gtcagaccac aaactgccgg cgtttcattt aaagcgtctt tttttcctca
       61 aaaaagatct tcaaccatca aatgagggca ttcaacgcaa ccaattagaa aaacaattgt
      121 taagaatttt cccttactct ttacttttta tagggtcagg caaagcagag acactctttc
      181 atttgatata ctagaaaaat aacccttcca attactggtt ttcacacagg aggtaggaat
      241 gaatcggaca agtcaacgcc gcatgatcca agagggaatc atccaaacag tttcgcgcat
      301 gatggggctt ttcatgctga ccatcgcctt gagtctgtct ggcgcagcag cctgggccga
      361 tggagacggt catggttaca gtcatgatcc aaaatccaaa ctggagaagc tcacgaagaa
      421 gctggatctt acgaaagaac agcaagacaa gatcctcccc attattgagg aaaaacacca
      481 gaagatggag ggacttcacc aacaaatgaa agacatgcgc caacaggcca tggctaaaat
      541 tgaagctgaa ctcactcctg aacaaaagga aaaatggaag gaaatgcaag aaaaacgggc
      601 ggaaaagatg aaggaataca aagaaaagca tggaaaagaa tgtgacaaaa aaggcaagca
      661 tggcaaggac gaagacgacg attaacgcct aggctaccca cgggggtcgg attaattcgt
      721 catccgggtt atctccgacc cccgctccca agccccttgg cttactcctg cttcttatcc
      781 tgctctcgaa aacgtttcag catacatcaa tctgtcccat cctccatcgc cctcaccgat
      841 ggtgataacg catagctttc tgtcatctcc gccatgagta agtagggatc tatgaataga
      901 tgccgttagc ttctagggaa tgactcatat ggagagacag aaaacaagat gaaggcccgc
      961 cgataccacc cgccggacat agtgggaacg aaccggtaca aatccaagaa tgcctggcaa
     1021 cacagaagcg aaagaaatcc tgatgatttt ccttaatttc ctaatagacc acataattgg
     1081 tagggtagaa ccatccataa gaggaaacgt actgtatagg gggctcaatt catgcttgaa
     1141 atggtagaaa ttcgggtcag ttatcgatat gtgaaagaac attcctgggt cgtacagggc
     1201 atcacaggat ttctttctgc ctatttcatg gaaaaaccag gatttgcatt aaaacgccac
     1261 tttgaagaat tggaaaccgg catgcacgtc tggttgtgcg atatcccgcc caaaatgaaa
     1321 gttcccacgc tcctgaggcg gttgaaggat gacatccctc cctgccaata taaacagctc
     1381 gaaaccagcc cgccggcgcg accgcaattt cttatcgatt cactggagtc ggaaaccgca
     1441 acctcctaac ctatttccaa aacaaggatt tcgaacggat agggggcgga aggagaacag
     1501 ggcatcacga aaacccctgt gagatgtgct gaaaatttaa ttgagtaggc tgagaagact
     1561 agagggattg tgtgggatcg atgcacaacg ccgaccgaag aaggtgaatc cgttcggcca
     1621 gttgttcagc gggaagccct acgggctcat gcggcacaca ccacaccggt tttaacggat
     1681 cgggatcggt cttgagcctt ggaatcagat gccagtgcat atgaggaacc tgattaccga
     1741 gcaattcata gttgatcttc ttcgcgtcaa acacctgcgc cagtgtctgc gccacgtggc
     1801 taacttcctc catcaacatg ctgcgcgcca ctctggataa atcaaacagt tcggaaacat
     1861 gctccttcaa caccaacacc gtccatccgg caaaaaactg gtcctcaaac acatacgctc
     1921 tggtcagacc acaatcgaca agaaaatgat ccttgagcgg ccatgctcca acacatattt
     1981 tgcaagaggg ttcggtcacg gcaatacgaa aagcgagggg atcaagaatt caccatgaga
     2041 gaaaaaagat tttatagacc taccagggaa tagacgaacc caattgactc atgacacttt
     2101 ctcctattct gggacaacat accgcacctt cccttcaccg ttaaagtctt aattcttctt
     2161 catggtatat tggaatgtgt ggagaatatg taaaaaaatg accatagaaa ccaacaattc
     2221 agagacgttc cagagttgtt acctgtttgc catcagcagt acatcttcca tcgtgttgcc
     2281 acttccttat gtggagaagg aatccatatg acaatagaaa ctcatcgctt gtccagaaga
     2341 accctgttaa agcggggaat gggcatcttc ggcttattag ctggaggttt cagtggcgcc
     2401 tccagccttg cagaaatact caaggagccc actccacgcc aaaccctggg gcccttcttc
     2461 cccgacgagg gagatccgat cgatgccatt cgcgaaaacc acgccatcgg cattcccatt
     2521 agtcaggcga acgatcagga tctcacgttt gtcaaaggcc gcaggggaaa agccaagggg
     2581 caggtcattt atttgaaagg aaaagtcctg agcgcaaaaa gcggcaagcc gatacctcac
     2641 acctcgatca tcatgtggag cgcctcggcc tccggccggt ataaccacaa agaggatggc
     2701 ggcatgttga aattccccca tcccacgacc ggagaaatca ttcaccgaac ctacgatgcg
     2761 tattttcaat actggggaca agtggtcacc aatgagcagg gagactattg gttcaaaacc
     2821 atcgtgcccg gcttttatcc tatcgacctg caagccggcc tgtaccgccc gtcccaccta
     2881 cactttcaac tggtacctcc agggcacccg aaactggtca cacagctcta tttccgtggc
     2941 gaccaaatcc ccaacaatga actgaaccag aaattactgc caatggatgt ggtcattctg
     3001 gatgccgggc tcac
//
LOCUS       DAORAQ010000292         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4568_length_3001_cov_66.809572, whole genome shotgun sequence.
ACCESSION   DAORAQ010000292 DAORAQ010000000
VERSION     DAORAQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_3001_cov_66.809572"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..894)
                     /locus_tag="PKM72_14330"
     CDS             complement(82..894)
                     /locus_tag="PKM72_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNP62021.1"
                     /translation="MKGLEFGIEQFRVTEEPFYQEVNGEIDLFSVGAKNKIPVMLKGP
                     TGCGKTRFVQHMAHRLNRPLITVACHEDLTASDLIGRYLLKGEETVWVDGPLTLGVKH
                     GALVYLDEVVEARKDTTVLIHPLSDDRRFLPIEKKGQILEAVDDFLLVISYNPGYQSV
                     LKELKQSTKQRFMAIEFGYPPKDIETRIVEHEAQVSRDIAVRLVKLGEKVRNLRNHGL
                     EEGVSTRLLIYAGTLMQQGVAPERACDAAITRPITDDTDMQRSIQELVKAIF"
     gene            complement(1030..1986)
                     /locus_tag="PKM72_14335"
     CDS             complement(1030..1986)
                     /locus_tag="PKM72_14335"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNP62022.1"
                     /translation="MSIHLSPLTLKDQVHLNQAIASTSIGGKTPLSTWSFPPHYIWKD
                     LFTFSWTDIDGWLCLFAEYSDGIFMPLPPVGPCSDSGSSSGSPLKNVLRQVMAYMEIR
                     NRGSHVTRIENIPAALKEEIQGWGYTLTPKDSDYLYRTADLIHLKGNPYKSQRAACNR
                     FIRTHRIRIAPYQSTDREECLALFHRWVHQKENIPISRLGPNEDVARMMLRDSANAHR
                     VTLQEHRELGLAGRIVWVDGAVKAYTFGFPQSSEVFCVLLEIADRSVYGLAQYLFREF
                     CREFQAYPLINTMDDSGLPALARTKLAYHPCQLMPNYIAQPS"
     gene            complement(2043..2666)
                     /locus_tag="PKM72_14340"
     CDS             complement(2043..2666)
                     /locus_tag="PKM72_14340"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNP62023.1"
                     /translation="MPQTIPVIPGPQVVPSAVCFRCDVCCRFPEQDSFLRPYFTEDEI
                     RQAVTHGILSSSFPDHRGSQIEVVRHPNDEGFLCPAFDPITQHCRIYEVRPLDCQLYP
                     FALMWNAQHEKVVLGWDTLCPFLLEQAGEENPFRKADPQPSALTIPKAIMEQAQRVAI
                     YLESDNVLNALAGHPRLVTPFQPDVVVLHTLDRLTETLQSSTTHDKR"
BASE COUNT          758 a          695 c          801 g          747 t
ORIGIN      
        1 gggggaaaat catgaaggat ttgatggaat cgtattcgaa gctcttgtaa gcatctgagg
       61 tgttacagta atcctgatgg tctagaaaat agccttcacg agttcctgaa tgctgcgttg
      121 catatcagtg tcgtccgtaa tcgggcgggt tatggcggca tcgcaagccc gctcgggggc
      181 aacgccctgt tgcatcaagg tcccggcgta aattaatagt cgggtactca cgccttcttc
      241 caggccatgg ttacggagat ttcgtacctt ttcacccaat ttgactaacc ggacggcaat
      301 gtccctggag acctgggctt catgctccac tatcctggtt tcgatatctt tggggggata
      361 accaaattcg atggccatga agcgctgctt ggtgctttgt ttcaattctt ttaagacgct
      421 ttgatatccg gggttatagg agatcaccag taaaaagtca tccacggctt caagaatctg
      481 tccttttttt tcaatgggca aaaaccgtcg gtcatcactt aatggatgga tcagcaccgt
      541 cgtatctttt ctggcttcga ccacctcatc cagatagacc agggcgccat gtttgactcc
      601 caaagtgagc ggaccatcca cccacacggt ttcttcacct tttaataggt atcgtccgat
      661 caaatcggag gccgtcaaat cttcatggca ggcaacggtg atcaagggcc gattaagccg
      721 atgagccata tgctggacaa accgggtttt cccgcatcca gtagggcctt tcagcatgac
      781 ggggatcttg ttttttgccc cgacggagaa caaatcaatt tctccgttca cttcctgata
      841 aaacggctcc tcggttaccc ggaattgttc aataccgaat tcaagaccct tcataaatca
      901 atccaatgtc atgtaaggat gaaaaagtga aacaggtgaa tgacttgacc agcattatac
      961 ggatgttaaa tcagagagga caacctgcaa atgctatcca tgaaatttca aatgggagtt
     1021 cacgaacgct catgaaggct gggcaatgta gtttggcatt aattggcagg gatgataggc
     1081 cagttttgtt cgtgctaaag ccggcagacc tgaatcatcc atcgtattta tcaatggata
     1141 ggcctgaaac tcgcgacaga attcacggaa aagatattgg gctaacccgt agactgaacg
     1201 atctgctatc tccagcaaga cacaaaacac ttcagaggat tgtgggaacc cgaacgtata
     1261 ggctttgaca gctccatcca cccacacgat tcgcccggca agaccgagtt cacgatgttc
     1321 ctggagggtg actcgatggg cattagcgga atcccgaagc atcatgcgag ccacatcttc
     1381 gttgggtcct agtcgggaaa tagggatatt ttccttttga tggacccaac ggtgaaaaag
     1441 ggcgaggcac tcctcacgat cggtgctctg gtaaggggca atacgaatgc ggtgtgtgcg
     1501 aataaatcga ttgcacgcgg cgcgttggga tttgtaggga ttgcctttca ggtgaatgag
     1561 atcggcagtg cgatagaggt aatccgaatc ttttggtgtg agggtgtatc cccatccttg
     1621 aatctcttct tttaaagcag cgggaatgtt ttcgattcgc gtcacatggg atccacgatt
     1681 ccggatttcc atatacgcca tgacctgcct tagcacattt ttgagagggc tgccggatga
     1741 ggatccactg tctgagcaag gtccgacagg gggcagaggc ataaagatcc catcggaata
     1801 ttccgcgaag aggcatagcc agccgtcaat gtccgtccag gaaaaggtaa acaggtcttt
     1861 ccagatataa tggggtggaa atgaccaggt actcaagggg gttttccccc cgatagatgt
     1921 ggaggcaatg gcctgattca gatgaacctg atcttttagg gtcaagggac tgaggtgaat
     1981 ggacatggat gaaagtttta cggggatgga gggtctggga gaaaagagtg agaccgtggc
     2041 cgtcaacgct tatcgtgtgt agtggaggat tgaagagtct cagtcaggcg atcgagtgtg
     2101 tgcaacacga cgacatcggg ttgaaacgga gtcactaatc ggggatgacc ggctagggca
     2161 ttgagaacat tatccgattc cagatagatc gccacacgtt gggcctgttc catgatcgcc
     2221 tttggaatgg tcagcgctga aggttgtggg tcagctttcc tgaaagggtt ttcttccccg
     2281 gcttgttcga gaagaaacgg gcacagagta tcccatccaa gcacgacttt ctcgtgttgg
     2341 gcattccaca taagtgcgaa gggatataat tgacaatcca gaggacgcac ctcataaatg
     2401 cgacagtgtt gtgtgatggg atcaaaggcg ggacagagaa atccttcatc attgggatgt
     2461 cggacgactt caatctgact gccccgatga tcaggaaagg aagaggaaag gatgccgtgt
     2521 gtgacggcct gtcgaatttc atcttccgta aagtacggtc tgaggaagct gtcctgttcg
     2581 gggaaacgac agcagacgtc gcatcgaaaa cagacggccg agggaacgac ttgtggacca
     2641 ggaatgacgg ggattgtctg aggcatatgc acatcacttt catggcgact tggcccgcca
     2701 tttccttcga gggcgtgcct tgtctggttt caagagagcg tgctacgatc tccctattct
     2761 agcaaaaggg ccgtcaccaa ggtccaagct tttcatatta ggcccgattt gaagtatttc
     2821 gtacaggtcc aacctctttt gatcgacgag tgaaggacgt cagagtgtct ggtccgtcac
     2881 atacgtcttc cacgattaac ccaggatcgg ttatttcttc ccaagtagaa cagtccggcc
     2941 ccattctgcc attgagtccg tcaacatctg acagccccta tcctctctat cgggacaggc
     3001 t
//
LOCUS       DAORAQ010000293         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4592_length_2990_cov_69.968313, whole genome shotgun sequence.
ACCESSION   DAORAQ010000293 DAORAQ010000000
VERSION     DAORAQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_2990_cov_69.968313"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PKM72_14345"
     CDS             complement(<1..1246)
                     /locus_tag="PKM72_14345"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNP62024.1"
                     /translation="MSTSPVISHPPIHTAPIPQEILEEIETFEDEVNRLQSGDTPSDV
                     FKPFRLQYGIYGQRQPDVQMVRIKIPFGGLNANQLRQVADIADTFATGVGHVTTRQDI
                     QLHFVPLRHVGTIMRKLAEVGLTTREACANTVRNVTACALAGVCPGEVFDVTPYAKTV
                     AYHLLRNPLNQSLPRKFKIAFSGCKQDCALTPIHDVGLLAARNSEGVPGFKMVVGGGL
                     GSTPRLAKVLHEFVPMEELIPATEAMLKVFDNLGNRKNRSKARMKFVVDKLGFEEFSK
                     RWKETYDAMLQSGATKFGLETAPYPDDPPLIMPTKPGNGTSHQGNGQSQLQGSSINED
                     PASQEAYDHWCATNVITQRQTGFRTAAVRLPSGDITSEQMFALAELTERYANGNLRTT
                     INQNIMVRWVPDIRLRHFYDELV"
     gene            complement(1347..1805)
                     /locus_tag="PKM72_14350"
     CDS             complement(1347..1805)
                     /locus_tag="PKM72_14350"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNP62025.1"
                     /translation="MNKFPIKVTYGILATIELARQDRSIPLQAKIIAQRQGIPSRFIE
                     QILQHLKQAGIIRSLRGAQGGYTLAQHPNQISLADLVNSMNGWATEPLLQQGHANGHS
                     ESRQAHNVLLSNIWQQVQEAEQAVLRGISLQKLVEQYQTLETQRCVMYHI"
     gene            2102..2488
                     /locus_tag="PKM72_14355"
     CDS             2102..2488
                     /locus_tag="PKM72_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62026.1"
                     /translation="MKDIADLLEEVQSIVQAMRGEKMMLEERLKTLEDDVSTMEKSKE
                     AMTRELSETKEKLGRELQATKKKLDQVHPEIQSLQDELQNVEVQNNLLVMDLKQKDEE
                     IKTLELRLQGYTSLTSPSFRIEEPIG"
BASE COUNT          777 a          653 c          764 g          796 t
ORIGIN      
        1 cgactaattc gtcatagaag tgccggaggc gaatatcggg aacccaacgt accataatgt
       61 tctgattgat ggtggtacga aggtttccgt tggcataccg ttcggtgagc tcagccagcg
      121 caaacatttg ctcagatgtg atatcacccg aggggagtct caccgccgct gttctaaatc
      181 ctgtctggcg ttgagtgatc acatttgtcg cgcaccaatg atcgtacgct tcttgagatg
      241 cgggatcttc attgatggat gagccctgaa gttggctctg tccattgccc tggtgggatg
      301 tgccgtttcc gggtttcgtc ggcattatta agggaggatc atcaggatac ggggccgttt
      361 ccaatccaaa tttggtggcg ccggattgca acatggcatc gtaggtctct ttccatcgtt
      421 tggaaaattc ctcgaaccct agtttgtcaa cgacaaattt catgcgggct ttgcttcgat
      481 ttttccgatt gcctaaatta tcaaacacct tgagcatggc ttcggtggcg ggaatcaact
      541 cttccatggg aacaaattca tgtagcactt tggcgaggcg cggagtggac cccaacccgc
      601 ctccgacgac cattttgaac cccggaaccc cttcagaatt gcgagcagcc agaagcccga
      661 catcgtggat gggggttaag gcgcagtctt gcttgcagcc ggaaaacgca attttgaact
      721 tgcgagggag gctttggttg agcgggtttc gtaatagatg ataggccacg gtttttgcat
      781 agggtgtcac gtcaaacact tcgccaggac acacccctgc caaggcacag gctgtgacat
      841 ttcgcacggt attggcacag gcttcccgag tcgtgagtcc aacttccgcc agttttcgca
      901 tgatggtgcc aacgtggcgg aggggaacaa agtgcagttg aatatcctgc cgcgtggtga
      961 catgtcccac gccggtcgcg aaggtatccg caatgtcagc cacttggcgt agttgatttg
     1021 cgttaaggcc gccaaaggga atcttaatcc ggaccatttg gacatccggc tgtcgctgac
     1081 cataaatgcc gtattggagt cgaaacggtt taaatacgtc ggacggagtg tcacctgatt
     1141 ggaggcgatt cacctcatcc tcgaatgttt ctatttcctc aagaatctcc tggggtatcg
     1201 gagccgtgtg aataggaggg tggctgatga ctggtgaggt gctcatgata gttctccaaa
     1261 tcctgtattc ggatcaaaat tttctatagg ttgctgagaa tgcactgatc tgttcgatct
     1321 tggtactgat gaatcttttg ccaaaactaa atatgataca tcacgcagcg ctgggtttcg
     1381 agcgtttgat actgttcaac caatttttgt aacgaaatgc cccgaaggac tgcctgctcc
     1441 gcctcctgaa cctgttgcca aatatttgaa agtaagacgt tgtgagcttg tcggctttct
     1501 gaatgaccat tggcatgtcc ttgttgaagc aatggttctg tcgcccatcc attcattgaa
     1561 ttcactaaat ccgccaggga aatttgattg gggtgttgag ccagggtata ccctccttga
     1621 gctcctcgca gactcctaat aatcccagct tgttttaagt gctgaagaat ttgctcaata
     1681 aaacgagagg gtatcccctg tcgttgagcg atgatttttg cctgaagcgg aatggatctg
     1741 tcctgccttg ctagctcgat tgtggccaaa atcccatagg tgactttgat ggggaacttg
     1801 ttcattttca attcatacta aaacgatagg aataatatgc aatataaaag gcgcagaaaa
     1861 catttgtcaa gaactgtgag ccgtactttt tctctcaaaa aatgcatggg attaaaaatg
     1921 ggtatggacg attgcctcct ggacggatct gtcatcagga gtccatggaa ttaatcaaac
     1981 gaaaagctga acaacaatcg catggtctct aaatattggt gaaaaatggg ccgatacctg
     2041 actgggaacg tattatgaaa agcaacggtt gcaacctttc ggagctgtga ggaccattga
     2101 gatgaaagat attgctgatc tgcttgaaga agttcagtcc atcgtccagg cgatgcgggg
     2161 agagaagatg atgctagagg aacgactgaa aaccttggag gatgatgtct cgacgatgga
     2221 gaaatcgaaa gaggctatga ctcgtgaact aagcgaaaca aaggaaaaac tgggacggga
     2281 actacaagct accaagaaaa aattggacca ggtgcatccg gaaatacaaa gtttgcaaga
     2341 tgaacttcag aatgtcgaag tacagaataa tcttctggtt atggatctca aacaaaaaga
     2401 cgaggaaata aaaaccttag aattgcgcct tcaaggatac acttcgttga cttctccctc
     2461 ttttcgaatt gaagagccca tcggttaagt ccgagggaat gccttccaat ttaaattccc
     2521 tggcgacggg atgaggcctt taattccgat caagagtttg tcccttcctg gatcgaattc
     2581 gtaactccag ccagttttct tcttcctgtt attatggtgt ctttagagga ccggcaagtg
     2641 tggggaagct cctctactta gtcacgttcc aataaggtgt gtgagacaaa aatagaaata
     2701 cgcgtagagt tgaaatcaca agtctgagcg ttgtgatgtc aggattcgtt gggtgaaaag
     2761 aggttcaccc ttatggtcaa tcttttcccc gttcattggg agaaataccc tgtcctttgg
     2821 cattaatgag gtggtctggg gacaaaggat tatctgctaa aaggatttga agctatccac
     2881 ggcatacccg attcgttcgt gagcaaggat tcgaagcatg ttgcctgttt ccttgaaaat
     2941 agaggaatgg aacctgtcaa ccattgtaca atgggctctc atcaccaggg
//
LOCUS       DAORAQ010000294         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4629_length_2973_cov_65.337217, whole genome shotgun sequence.
ACCESSION   DAORAQ010000294 DAORAQ010000000
VERSION     DAORAQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4629_length_2973_cov_65.337217"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1911
                     /locus_tag="PKM72_14360"
     CDS             211..1911
                     /locus_tag="PKM72_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNP62027.1"
                     /translation="MTYAVQESKFNFLHVKGDILGGLTAGVVTLPLALAFGLQSGLGA
                     VSGLYCAILLGAIAILFGGTRTLISTPTGPMTVVAALTISQAISFSGSLESAMGTIIG
                     IFILAGVVQIAFGLLKIGGNIKYIPYPVLSGFMTGIGIILILFEVFPLMGLSAPSTIY
                     GVFTGGAQALRDMNLHALGLGTLTLVILYTAPKVSTTIPAALMALLVGTFVALATGFS
                     VPLIGEVAQGLPTLQLESLWHVNVSQPSFIISAAFTLGALGCIDTLLTAVIVDKITET
                     KHQSNRELIGQGMGNVASALFGGLPGAGTTMSSIVNVKAGGRTQLAGVISSLFLLAVL
                     LGLGKYVQYVPIPVLAAILITVGLDIIDYKGLKEVVKVDRAESAILFIVLFLTVFVDL
                     ITAVGAGMTLSVFIFMKKMGDIGEEKIVLFPLRSLKIRKPCDLREEFVLPQDYLETVY
                     IKTFTGPIFFGFSHFLIDNVKQLPSVKILIFEMNRVPYLDQSAAHALELVFEYMQKRD
                     IRVLLANINPQPLEMLRAVDLTPRLIPAHHMFDDIFDCVRWLEEEFVSGETPLNPRVI
                     "
     gene            2003..>2973
                     /locus_tag="PKM72_14365"
     CDS             2003..>2973
                     /locus_tag="PKM72_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNP62028.1"
                     /translation="MTTPSIKIPGFSIPTIGYVVLIVGVALIISLVDGLFPLGVSISE
                     AYTVLVLLGFMAKDKRLIYGGAIAGTILTLEGAFISDPGLPLWIGEINRALSIFVIWL
                     VAVFALGQLRFMEEQKESEKMKMAYELLKQETTFLKLNQEIAVLSNTNDPVEDALKGA
                     MKIICDYTGWPVAHLYIRDGDTDLLSPSKIWVLREWGQFERFREVTEATKFGPGEGLP
                     GRVLASGKVAWIKNVTKDPNFPRARLAKEIGVKAGFAFPILIGPKVVAVMEFFSEEAM
                     EPNAKLLDVMEIIGYLLGRIFERDHAGLKRGEYEDHLRRLYSRIKAVR"
BASE COUNT          671 a          684 c          795 g          823 t
ORIGIN      
        1 cctgcaatcg gacatcgttc ccatcattcc ccaagcttgc tttgggttta aaacatttgg
       61 ctcatagcct atactatcgc gtcgttggct ggtgtcacgc gaaacacata tacaggctgg
      121 caaggctccg gacggctttc aagcaacacg gctttcaaac aacgcatggg tgtaggactg
      181 aagtcctgac cggatgatcc caaatttcct atgacatacg cagtacagga atcaaaattt
      241 aattttcttc atgtaaaggg ggatattctc ggcggtctga ccgctggagt ggtgacctta
      301 cctttggccc tggcctttgg ccttcaatcc ggactgggag cggtgtccgg gctgtattgt
      361 gcaattctgc tgggagccat tgccattctc tttggcggca cgcgcacgct catcagtact
      421 ccaaccggcc ccatgacggt ggtcgccgcc ctgaccattt ctcaagccat cagtttctcg
      481 gggagcttgg aatcggccat ggggacgatt atcggaatat tcatcctggc gggtgtggtg
      541 caaatcgcct ttggactcct caaaatcggc gggaatatca aatatatccc ctatcccgtg
      601 ctctcgggct ttatgaccgg cattggcatt attctgatct tatttgaagt gtttccgtta
      661 atgggcttgt cggctccctc taccatctat ggagtgttca ccgggggggc gcaggctttg
      721 cgtgacatga atcttcatgc gcttggtctt gggacactga ccctggtgat tctgtatacg
      781 gctccgaagg tcagtaccac cattccggcg gcgctgatgg ccttgttggt tggaaccttt
      841 gtcgcattgg cgacgggttt ttctgttccc ctcatcgggg aggtggctca agggttgccg
      901 acccttcaac tcgaatcgtt atggcatgtg aatgtttctc aaccatcttt tatcatcagc
      961 gcggccttca cccttggagc cttgggatgc attgacactc tcttgacggc tgtaattgtc
     1021 gacaaaatca cggaaaccaa gcatcagagt aatcgtgaat taattggtca gggtatgggc
     1081 aatgtggcat cggcactctt tggtggatta cccggtgcgg gaacgaccat gtcgtcgatc
     1141 gtcaatgtga aggctggagg tcgtacacag ctcgccgggg tgatttccag tctttttctg
     1201 ctggcggtgt tattaggctt ggggaaatat gttcaatatg tgcccattcc cgttctggcg
     1261 gccattctca ttactgtcgg tttagacatc attgactata agggcctcaa agaagtggtg
     1321 aaagtggatc gggctgaaag cgccatcctc tttattgtgt tgttcctgac cgtgtttgtt
     1381 gatttgatca cggccgtcgg ggcagggatg acgctttcgg tttttatttt catgaaaaaa
     1441 atgggggaca tcggagagga aaaaatcgtt ttgtttcccc ttcggtcgct aaaaattcga
     1501 aaaccctgcg acctgagaga agaattcgtc ttaccccagg attatttgga gacggtgtat
     1561 atcaaaacgt ttacggggcc gattttcttc ggcttttctc attttttaat cgataacgtt
     1621 aaacaattgc cctcagtcaa gattcttatt tttgagatga atcgcgtgcc gtatctcgat
     1681 caatccgccg cgcatgccct ggaattagtc tttgaataca tgcaaaagcg ggatattcga
     1741 gtcctgttgg ccaacattaa tccccaaccg ctggagatgc tccgcgcggt tgatctgacg
     1801 ccaagactca ttccagcaca tcatatgttt gatgatattt tcgattgcgt ccgatggctg
     1861 gaggaagagt ttgtgtcagg cgaaactcct ctgaacccac gggttattta actctagaaa
     1921 gtcgattggc aaaaaaggtg tcggcaaaaa atgaccacgc tcacgaatct caatcacact
     1981 gtggagccat cgtaggtacc ttatgaccac accctcaata aaaattcccg gtttttcgat
     2041 cccgaccatt ggctatgtgg tcctcatcgt cggtgttgcg ctgatcattt ctcttgttga
     2101 tggcctgttt ccgttgggcg tgtcaataag tgaagcctat acggttttgg tgttattggg
     2161 cttcatggcc aaagataagc ggttgatcta tggcggggcc attgccggga cgattctcac
     2221 cttggaaggg gcgtttattt ccgatccagg cctgccttta tggataggag aaatcaatcg
     2281 ggcactttct attttcgtca tatggttggt ggccgtgttt gccctggggc aattgcggtt
     2341 tatggaagag cagaaagaat cggaaaaaat gaaaatggcc tatgaactct tgaaacagga
     2401 aacgaccttt ctgaaattaa atcaggagat tgccgtctta tccaatacca atgaccctgt
     2461 agaagatgca ttaaaaggtg ccatgaaaat catttgcgac tataccgggt ggccggtcgc
     2521 acatctctat atccgtgacg gcgatacgga tctgttgagt cccagcaaaa tttgggtttt
     2581 gagggaatgg gggcaatttg aaaggtttcg agaggtgacg gaagcgacga aatttggtcc
     2641 tggagagggt ttgccaggtc gggtgttggc tagtggaaag gtggcctgga ttaaaaatgt
     2701 gacgaaggat cccaatttcc ctcgcgcccg cctggcaaag gaaataggcg tgaaggccgg
     2761 ttttgcgttt ccgatcctca ttggaccgaa agtggtggcg gtgatggagt ttttctcgga
     2821 agaagcgatg gaaccgaatg ccaagctcct ggacgttatg gaaatcatcg gctatctgct
     2881 gggtcgcatc tttgaacgag accacgctgg gctcaagcgg ggcgaatatg aggatcactt
     2941 gagacgtctc tacagccgca tcaaagcggt gcg
//
LOCUS       DAORAQ010000295         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4636_length_2970_cov_57.622642, whole genome shotgun sequence.
ACCESSION   DAORAQ010000295 DAORAQ010000000
VERSION     DAORAQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4636_length_2970_cov_57.622642"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKM72_14370"
     CDS             <1..378
                     /locus_tag="PKM72_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNP62029.1"
                     /translation="MKRVLNIVLCVLLGIFFIAAGGAKLMGSESQVEHFAQWGYPIWF
                     LYLTGVIEVGGGLCLFIPKTQWYGMVVLSITMVGAALTHLKAGEMGAVPVPLVLLGLL
                     LLLAWMIREPSRKNGANQWPPQK"
     gene            728..>2970
                     /locus_tag="PKM72_14375"
     CDS             728..>2970
                     /locus_tag="PKM72_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62030.1"
                     /translation="MIPTFGLARSVFLRKFPIAVTAMTMMILACTFGMLSVHAGQHPH
                     SQTLRGTVEGNGHAQRGYKVSLYAAEAGGHGKGQVLGTARTDGDGKFKIRYRVPHGQQ
                     FVLFVIAEYGPVMLAGVIGTGHHVYDAVVVNERTTVATGTAFAQFVDLWKIHGNTYGM
                     INAVKMAENMANPETGDIGEVLFNTPNGTETSTYPTFNSLTNIVASCVADDTNCYSLF
                     MATTPEGGPAPTTVLQAVANIAKYPSYPGYPTDTDDPLYQLSLLDPVNQPALMKRPTS
                     WLLFIKFNGGQHNIYDPFNLMSGPGQIAFDEGGFAWINDNYRPTFSNEIACAGQRLIK
                     TYPWGETFPGSPYFGGGLSGAGFGIGLDPHGKVWVGNFGFEAPACADTTVEPLPDPAL
                     KIPAAHDSVSVFRPDGSPISGFDGFTEGHIWWPQATVSDKKGNIWIANCGNDTVTFIP
                     KGKPGHARNIAFPGGRGAEGIYAPTIPDTEPDSDAPLLKPFAIAIDPKGRAWVTANQV
                     GYVADSGTADPTEAIGGVYRVSSDGTVEMLPNLNAKSGPVLSWPMGISGDSQGNMWVS
                     NSNSVKVPCVDPLDPQDGMDGPSVTLYPADDSAPSTHIGGGLTIPWGNAVDGNDTLWV
                     FNFGQKPTRDVDENTVWPDTPLSHFCGADPSKCPSGKGTGDPISRDPGYVSDALDRVT
                     GGGIDPSGNVWLLNNWKKTGPFGPVYNTNPGGNSFVIVPGAAGPVKTPLIGPPQSFDQ
                     RGHRRDHR"
BASE COUNT          641 a          807 c          833 g          689 t
ORIGIN      
        1 atgaagcgcg tgttgaatat tgtcctatgt gtgctgttgg ggattttttt tattgcagcg
       61 gggggagcca agctcatggg cagtgagtcc caggttgaac attttgcgca atgggggtat
      121 ccgatttggt ttttgtattt gaccggggtc attgaagtcg gcggcggact ttgtttgttt
      181 attccgaaga cgcaatggta tggcatggtg gtcttgagca tcaccatggt gggtgcggcg
      241 ctgacacact tgaaggctgg agaaatgggt gctgtgcccg ttccacttgt gcttctgggt
      301 cttttgctcc tgttggcgtg gatgatcagg gaaccgtcca ggaagaatgg agccaatcaa
      361 tggcctcccc aaaaatgaat ggcctgaagc ttcatcaatg gtattaccgc ccaaaattct
      421 caaaaaatct cttgagttgt ttattcacat ttcagtaggg attcgtttgt agggctcttc
      481 tgtgttggct gtgcctgcat caaacgttgg gcttgccaag ctgtgatgtg tttgaataac
      541 tcgccgctga cccatcaata caggggcttt ttgtgctgcc ccattgctgg ttgaccactc
      601 acatttttcg gactcctttt ttctttccta atcttgaccc ttcaagcggt catttcctac
      661 cctcagtaac aggcaacatt gaccattttt cccatgtgca gagggaagag aataaggagg
      721 tctgatcatg atacctacat ttggactcgc gcgatcggtc tttttgagaa aattcccgat
      781 agctgtcaca gccatgacca tgatgatact ggcgtgcacg tttggcatgc tgtccgtgca
      841 tgctggccaa catccgcatt cccaaacgct ccgtggcact gtagagggca atggccatgc
      901 gcaacgcgga tataaggtct cgctttatgc ggctgaggca ggtggccatg ggaagggtca
      961 agtgcttgga acggctagaa ccgatggcga cgggaaattc aagatccgct accgggtgcc
     1021 gcatggtcag caattcgtcc tcttcgtcat tgctgaatac ggcccggtca tgctggccgg
     1081 tgttatcggc acgggccacc atgtttacga cgccgtggtg gtcaacgaac gcacgacggt
     1141 tgccacggga accgctttcg ctcagttcgt cgacctctgg aaaattcacg gtaatactta
     1201 cggcatgatc aacgccgtga agatggcgga gaacatggcc aatccggaaa ccggagacat
     1261 tggcgaagtg ctcttcaata caccaaatgg aaccgagaca tcgacatacc cgaccttcaa
     1321 ttccctcacc aacatagttg cgagttgcgt cgcggatgac accaactgtt actccctgtt
     1381 catggcgacg acaccggagg gaggacccgc gccgaccacg gtgttgcaag ccgtcgccaa
     1441 catcgccaaa tatccgtcgt atccaggtta tccgacagat acggacgatc cgctctatca
     1501 gctgtcgcta ttggatccgg tcaatcagcc ggcattgatg aaacgtccaa ccagctggct
     1561 gttgttcatc aaattcaacg gggggcagca caacatctac gatccattca acctcatgag
     1621 cggccctggc caaattgcct ttgacgaagg gggcttcgcc tggatcaacg acaactatcg
     1681 tccgacattc tcaaatgaaa ttgcctgtgc cgggcagcgc ctgattaaaa cgtatccctg
     1741 gggtgagacc ttccccggat cgccgtactt cggcggtggt ctcagcgggg cggggtttgg
     1801 catcgggctg gacccgcatg gcaaggtctg ggtgggcaat ttcggatttg aggcgccggc
     1861 ctgtgcggat accaccgtcg aacctctgcc tgacccggca ttaaagattc cagccgccca
     1921 tgatagcgtc tcggtgttcc gtcccgatgg cagcccgatc tccggcttcg atggcttcac
     1981 tgaaggccat atctggtggc cccaggctac cgtctcagac aaaaagggga acatctggat
     2041 tgctaactgc ggaaacgaca cggtcacatt cattccaaaa ggtaaacctg gccatgcgag
     2101 gaacatcgcg ttcccgggtg gacgaggagc agaaggcatc tacgccccca ctatcccgga
     2161 tacggagccg gattcggacg cgccacttct caaaccgttt gccatcgcga tcgatccgaa
     2221 gggccgcgcg tgggtaaccg ccaaccaggt cggatatgtg gccgattccg gcacggccga
     2281 tccaaccgag gcgattggcg gggtctaccg cgtgtcgtcc gatggcacag tggagatgct
     2341 tccgaacctc aatgccaaat ccgggccggt gttgtcctgg ccgatgggaa tttccggcga
     2401 cagccaggga aacatgtggg tctctaactc gaattcggtg aaagtgccct gtgtcgaccc
     2461 actcgatcct caggacggaa tggatggtcc ttcagtcact ctgtatcccg ccgatgacag
     2521 cgctccctct actcatattg gtggcggtct gacgattccc tggggcaacg cggtcgacgg
     2581 aaatgacacg ctatgggtgt ttaattttgg acagaaacca acccgcgacg tcgacgagaa
     2641 cacggtgtgg cccgacacac cgctttcgca tttttgcggt gcggatccca gcaaatgtcc
     2701 ctcgggtaaa ggcacgggcg atcctatctc acgggacccc ggttacgtca gtgacgcgtt
     2761 ggatcgggtc acaggtggtg gtatcgatcc gtctggcaat gtctggctcc tgaacaattg
     2821 gaaaaagacc ggtcctttcg ggccggtcta taacaccaac ccagggggca attccttcgt
     2881 gatcgttccc ggcgcggccg gacccgtcaa gacaccattg atcgggccac cgcaatcatt
     2941 tgatcagcga ggccacagga gagaccaccg
//
LOCUS       DAORAQ010000296         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4662_length_2956_cov_69.392279, whole genome shotgun sequence.
ACCESSION   DAORAQ010000296 DAORAQ010000000
VERSION     DAORAQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4662_length_2956_cov_69.392279"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..256)
                     /locus_tag="PKM72_14380"
     CDS             complement(68..256)
                     /locus_tag="PKM72_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62031.1"
                     /translation="MGNFFTNDQGTAAIEYSLLLSLIGMAVIGSLQLLGTNVFDGIST
                     LNQVFVSTQLKDDSLPHP"
     gene            1158..1613
                     /locus_tag="PKM72_14385"
     CDS             1158..1613
                     /locus_tag="PKM72_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNP62032.1"
                     /translation="MLVQEVMSTGVVTAFPTDSVRSVVIKMLSRHCGAVPVIDEHQNL
                     IGLVALRDVMLPLFPNFGDYIHDNVHSRDFTEMEEGYPDILRKKIESIMSKNPLTVAP
                     HTPILEAASFMGRKHFRRIPVAENGKLLGMISIGDINRGLFFQRGFLGQ"
     gene            complement(2054..2500)
                     /locus_tag="PKM72_14390"
     CDS             complement(2054..2500)
                     /locus_tag="PKM72_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNP62033.1"
                     /translation="MSGSRILVFSLSIFWLVSLPWSSYSAESDFATHDSDHEDDREVE
                     TLPAVHVHGLRLNKDQQIGPVPQRTPWPTVPPTLDGQVLDDWLKARILVSKSSEVTVV
                     ILQPATHRELTQSSLAALKQWTFLPQMDGDEPIDGEVSLRIHFRTQ"
     gene            complement(2507..>2956)
                     /locus_tag="PKM72_14395"
     CDS             complement(2507..>2956)
                     /locus_tag="PKM72_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62034.1"
                     /translation="NVAWGQCRGKKLQSGFMLLEQEEINDINSSSGVYCDHMQLVTSS
                     AVLTYRPFTKTTITGQMLFGSGMRTTDGGGKTNATHSPSYTTYNLSIEHLLPLWDKYK
                     LLLGFDVINLLDQHVFLNDGEGSIGLGVAHANMPRSFFFRGQFFFGG"
BASE COUNT          908 a          677 c          621 g          750 t
ORIGIN      
        1 ggaggaaggc acaaggaagg gaacccctta ggtcgcagag ggttaaggta tggtatgaaa
       61 tttctctcta agggtgaggc aacgagtcat cctttaattg ggtcgataca aacacctgat
      121 tcagagtaga aataccatca aacacattgg tgcctaataa ctgaagagat ccgataaccg
      181 ccatcccgat taaacttaaa agtagggaat attctatcgc tgccgtcccc tggtcattgg
      241 taaaaaaatt acccatgctc gcaatctcct tctaaaacca cttttgttct gtaggctttg
      301 ctgtatacaa ccataatgca aacggtaaac cgaattccca gaacgcctcg tttgcattac
      361 ttccacttgc ttgtctgaat tctcttctcc cagctagtcc ttacgaggaa gagtcggaaa
      421 cacccgtgcc ttactcagtt gacaaacacc tcgacacatc ctggaaagac gcagataatt
      481 ccgccaacat ctaaataaaa gttcctcata gtcaacaaaa cagatgcaaa atttgtcagc
      541 gaatatgaca aatttgaaat aaaggccaaa aagatctaac agattatgta tttttcaaat
      601 aagagtagat cagaaattgg aggtattctt gatgaccagt cggaacgagg ctctaagata
      661 atccctgatc caatagtatc gaattttgac tatcccaact tttttgaata ggaaggttca
      721 tcataggctc atcgactcgt tatggtaggg gtactgcaat ccacgccggg agctaattgg
      781 gatgagttcg aatggtctca ccaagatttc ggagttaatc gaaattttcc acctcaagaa
      841 ggcattttca gagggatcct cagaacttgt ctccctaacc tgcacggtag caaaatccga
      901 gatgcctgat ttgagaggca caaaaaaaac ccttaattct cttccaaccc cttcagacct
      961 tgttattgcg gagaatgaaa agaacgccgt aaaattcaaa ggtaaaaaac actcccaata
     1021 ttgttgttta aaattgcact tatttaaaaa aactggcttc acgatggatg catcaggagg
     1081 agaaaagaaa ttccctcatg actctgtggt tccgtctggg ggatatccct ctatcatgtt
     1141 cacagaagga ggatgccatg ttagtccaag aagtcatgtc cactggagtc gtaaccgcgt
     1201 tcccaacaga ttcagtacga tcagtcgtca ttaaaatgct cagccgccat tgcggagcag
     1261 tacctgtcat tgacgaacat caaaacttaa tcggactcgt cgcccttcga gatgtcatgc
     1321 ttcccctctt ccctaatttt ggggattata tacatgacaa tgttcatagc cgagatttca
     1381 cggaaatgga ggagggatac ccggacatcc ttcgaaaaaa aatcgaatca attatgagca
     1441 aaaatcccct taccgtagcc ccacataccc caattcttga agccgcctca tttatgggga
     1501 gaaaacattt tcgtcgcatc ccggttgctg aaaatgggaa gcttctggga atgatcagca
     1561 ttggagatat taaccgtggc ctgtttttcc agagagggtt tctgggtcag taacctgaat
     1621 caatcgtcaa gcagcttcaa atataagatt aggacaaaca cgtgacagaa aaataaatca
     1681 aagaaacaat cacgtgtctt gaagaggata agagcgaacg cgatttacct tgaagcgtct
     1741 ccgctctatc gactatttac aacggcttgg acaaatacac ttggggaccc atagcaaaaa
     1801 aattttcttt attcattgta gaacgaaatc ctgcccacga agtaacggag aattctggac
     1861 atttcctcat gacttgatga gccagcaatt cggccggcat cccatgcatg ggtcgattcg
     1921 ccacaatcac atcaaattaa tcagtatact tcttgaaaga ctgcaacgtt tcaggaccat
     1981 tgggaaattg gtttctagcc atctgatgga aactggcctg caaattgtcc tgcatttagg
     2041 ggacaggaat ggcttactga gtgcggaagt gaatacggag tgagacttca ccatcaattg
     2101 gttcatctcc atccatttga ggtaagaacg tccattgttt gagagctgcc aatgaggatt
     2161 gagtgagttc tcgatgcgtg gcaggttgaa ggatcacgac cgtcacctcg gaagatttac
     2221 taaccaaaat tcgagctttg agccaatcat ctaggacctg cccatctaag gtgggcggaa
     2281 ccgtcggcca gggtgtccgt tgagggaccg gtcctatttg ttggtcttta tttagtcgta
     2341 atccatgaac atgaacagca ggaagagtct caacctctcg atcatcctcg tggtcagaat
     2401 catgagtcgc aaaatcggat tcagccgaat acgatgacca gggaagactc accagccaga
     2461 aaatggacaa agagaagaca agaattcgcg aaccactcat aaaaaattat ccaccgaaaa
     2521 agaattgccc acgaaagaaa aaagatcgcg gcatattagc atgtgccaca ccaagcccaa
     2581 tactcccctc tccatcattt aaaaacacat gttggtcgag cagattaatg acatcaaacc
     2641 caagcaacag tttatactta tcccacagag gaagcagatg ctcaatggaa agattataag
     2701 tggtatacga cgggctatgg gtggcattgg tctttcctcc cccgtctgtt gtccgcatcc
     2761 ccgacccaaa taacatttgc ccagtgatag ttgttttcgt gaacggacga taagttagga
     2821 cagccgaact ggtgaccagt tgcatatgat cacaatatac tccactgctg gaattgatat
     2881 cattaatttc ttcctgctct aacaacataa aacctgattg aagttttttc cctcgacact
     2941 ggccccatgc gacatt
//
LOCUS       DAORAQ010000297         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4676_length_2948_cov_66.855513, whole genome shotgun sequence.
ACCESSION   DAORAQ010000297 DAORAQ010000000
VERSION     DAORAQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4676_length_2948_cov_66.855513"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            579..1769
                     /locus_tag="PKM72_14400"
     CDS             579..1769
                     /locus_tag="PKM72_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62035.1"
                     /translation="MSGLGCRHYRNLIGLLGAFGLVLGCVSGPGGNEAYKKEGFQVTR
                     PNPGARVVLRGNHAAAVNQAFHWLNDHQLLVVDRRVDPNMTAQKFSRQNDTERQTQFL
                     AVDRMVGATLWVFAQVDETSLLNSTVDSMNGESQNLNAATVDIQGIDAKTGEVMFGAK
                     AWNSDPLVVSDQVIQDLTIVALEQAWQKSAVQPSSPQEVKSEDTTELPALQVAPVSAD
                     SSSSPTTTQSAKVYSESPGSATVAQSEPVESSATSPEQTPVIADPVSEERSPVSVETP
                     VSVEPSSDTPMTTQPEPADLEPSAEPAVDQPADAENSGNSDDSSLGLQVASGALSILY
                     TPLKVVYAGVGGIFGGFVYLVTAGNEQAAQSVWDASLKGDYWLTPDHLQGNEPVRFKG
                     ETTR"
     gene            complement(1766..2452)
                     /locus_tag="PKM72_14405"
     CDS             complement(1766..2452)
                     /locus_tag="PKM72_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNP62036.1"
                     /translation="MVHVWRVHLDMSPRQAALYRADLSEEERARAERYRLPHHQYQFI
                     STRGILRNLIGHYVGIPPSTLGLGTNLQGKPSLTEPSHPTLQFNVSHTSGMALLAFTV
                     KHAVGIDVEEVNRNVSDQDIATRYFSPREAAYLATLSPDKRTQEFLTYWTCKEAYLKM
                     QGIGLSGGMAQCEIALDQDGWKATVLSTHKPDRRNDSSLFRINAGQAHIGALAVACPS
                     VEVLFWDWKD"
BASE COUNT          720 a          695 c          776 g          757 t
ORIGIN      
        1 tcgccactcg ccaggtgacg ccttcgatga aaaatgacag aattcgtaaa gccatcacaa
       61 ttttaggaag tgagagtata tcgttcctgc aggccttacg gccgaatcag gcaattcact
      121 agcatactag ggaagcgggg gctcaagcaa ttccagccac ttttgatacg atggaaagac
      181 aatgtgagaa atgggttggc cttgttttat ttgacttgtt ttgtaagatt aaggagaaaa
      241 agtcagggaa tttactccgt gaaggcacca tttgtggtat tttaagagct ccggttctct
      301 gtagcgtgct tcggcgttac cgtatgagaa ctgtaattga ttgatcccct gaataccctg
      361 ctgcttgagg gatcttctaa ttttacataa gattttcatt tgctaatttt tcccctacca
      421 tatggaaggt atctccaatt tctttcctta ttctaatgtg actatttggc aagtaaacct
      481 ggtccggacc ttttttgagc ggggttcagg aaaaaattct tatctggtct acacatgcgt
      541 atttttacaa aagaattgat aacgaaggga acattgctat gtcgggttta ggttgtcgtc
      601 actatagaaa cctgatcggt ctgcttggag cttttggact ggtgttggga tgtgttagtg
      661 gtcccggcgg gaatgaagcc tataaaaaag aagggtttca agtgactcgt cctaatccgg
      721 gagccagggt ggtgttgagg ggaaatcatg cggcggccgt caatcaggcc tttcattggt
      781 taaacgacca tcaactactg gtggtcgatc ggcgggtgga tccgaacatg accgctcaga
      841 aattttcccg tcaaaatgac accgagcggc aaacccaatt tttagcagtc gatcgtatgg
      901 ttggggcgac tttatgggtc tttgcccagg tcgatgaaac ttcgttgtta aattccacgg
      961 tagattccat gaatggtgag tctcaaaacc tgaacgcggc taccgtagac attcaaggga
     1021 tcgatgcaaa aaccggggag gtcatgttcg gagccaaggc ctggaattct gacccactgg
     1081 tggtatccga tcaagtgata caagatttga ccatcgttgc ccttgaacag gcttggcaga
     1141 aatcagcggt ccagccttcc tcgccacaag aggtgaaatc agaggatacc accgagcttc
     1201 cggcacttca ggtagcgccg gtatcggctg attcttccag ttcacccacc acaactcagt
     1261 cggcgaaggt atattcagag tcgcctggat cagccactgt ggcccagtca gagccggtgg
     1321 aatcaagcgc gacttctcca gagcagactc cggtcattgc agacccggtt tctgaggaaa
     1381 ggtctcccgt gtctgttgag acaccggtat cggttgagcc atcttccgat acacctatga
     1441 caactcagcc agaaccggca gatttggagc cgtctgctga gccagccgtg gatcagccgg
     1501 cggatgccga aaattcaggg aactcagacg attcgtcctt gggacttcaa gtcgcgagtg
     1561 gagccttgtc gattttatat acgcccctca aagtagtcta tgccggcgtg ggtgggatat
     1621 ttggagggtt tgtttatctg gtgaccgcag ggaatgaaca ggcggctcaa tcagtttggg
     1681 acgccagtct taaaggggat tattggctca ccccggacca tttacaaggg aacgaacctg
     1741 ttcgcttcaa gggagaaacg acgcgttaat ccttccaatc ccaaaacaat acttcaaccg
     1801 aagggcatgc cacggctagc gctccgatgt gggcctggcc tgcattgatg cgaaagagtg
     1861 aagagtcgtt cctccgatcc ggtttatgtg ttgacagcac agtagctttc catccgtcct
     1921 ggtcgagtgc aatctcacat tgagccattc cacccgataa tccaattccc tgcattttga
     1981 gatacgcttc cttgcaggtc cagtaggtca aaaactcttg ggtccgcttg tcaggtgaaa
     2041 gggtggcaag gtacgccgcc tcccgtggag aaaaatacct tgtggcaata tcctgatcgc
     2101 tgacattccg gttgacctct tccacatcaa tccccacagc atgtttaacc gtgaaggcca
     2161 gcaatgccat tccactcgta tgagagacat tgaattgtaa tgtcggatgg gatggttcag
     2221 tcagagaggg tttcccttgt aagttggtcc caagtccgag agtactgggg ggaattccca
     2281 catagtgacc gatcaaattt cgaagaatgc ctctggttga aataaattgg tattggtggt
     2341 gaggaagccg ataccgttct gcccgtgccc gttcttcctc agataggtcg gcacggtaaa
     2401 gagcagcctg ccggggtgac atatccagat gaacccgcca cacatggacc acgcctggcg
     2461 acaggctcct aaaaatgtca ggagaggagg ggcaagccca gtgggtggaa ggcagcattg
     2521 atttcagagg gattgtatcg gaagggcggc ttggcttaaa ccctttgagc cttctataaa
     2581 gagtcgatga ccggtgtgtt ccacgttctt atttgtcgtc atctttccta tactgccgac
     2641 ttctgcaaga atatgagggt aggaaccagg tatccttgca gagttctcga gagggcaacc
     2701 accgaccaca cctgaaaggc tttcccagat gacagctcgg atcatatgtt ttatttaaaa
     2761 tcccggggtg ccacaactac attacattgg ttcttcggtt tcgtcgtgtt tactcttctt
     2821 gtctcatgtt cggagaaagg cctgtctgat atgacccccg ggcaactgtt agcctcaatc
     2881 gaaataggca gggcgccagt gatcgtggat gtccgctcgc aaagtgaata cgatgccggg
     2941 catgtgcc
//
LOCUS       DAORAQ010000298         2946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4682_length_2946_cov_65.291249, whole genome shotgun sequence.
ACCESSION   DAORAQ010000298 DAORAQ010000000
VERSION     DAORAQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4682_length_2946_cov_65.291249"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /gene="smbP"
                     /locus_tag="PKM72_14410"
     CDS             complement(<1..281)
                     /gene="smbP"
                     /locus_tag="PKM72_14410"
                     /inference="COORDINATES: protein motif:HMM:NF028095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNP62037.1"
                     /translation="MSSTFSTFIRIVGCVGVLGFLAVPAVTLAEGDKHLHEAIGHAGE
                     AKAHGEMGHAGEATKHAQEALSHADAAKQEGGNAHVGEGISHLGEAVDHG"
     gene            1153..2229
                     /locus_tag="PKM72_14415"
     CDS             1153..2229
                     /locus_tag="PKM72_14415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP62038.1"
                     /translation="MLNPSHGNSPHTFSPRMKWQRIFLCLIFLWPLPALGHQAGNLYR
                     DALSAVENQDFKTAQSLLEQAIQEFPGFSEAHHLYGLVEFQLTQQPERAIAALQQAIR
                     LNPNLAQGQYDLALLFIKQDQIEEAQKAAQQALAIYPRFWEARLILAKLLDKRELTNQ
                     AIQEYQTILTQQPFQEEALYHLATRFMQAQDFDQAQLLLTQLTEHHPHQTDAWLLLGR
                     IAERQNQPSQALHAYQEVIQANPDNSEAHANLGFLYQQQGNLPEAIEHFQRVSELKPQ
                     DAEAFLNLGVLFAGNHQLVEAEQAYQTGIALQPDSLEGHFNLGAFYEFHKKDLPQARI
                     HYQKYLDLGGKDARIQQLLTQLGQ"
     gene            2713..>2946
                     /locus_tag="PKM72_14420"
     CDS             2713..>2946
                     /locus_tag="PKM72_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNP62039.1"
                     /translation="MGRNSIKGLQNPLRNQQGAYILITGIAVVALFGFAALGIEVGRW
                     YAIQGEMSKAIDGAAFAGAKNVNNPNITDLHGFV"
BASE COUNT          782 a          808 c          582 g          774 t
ORIGIN      
        1 ccatggtcca ccgcttcgcc gagatgactg atgccctcac cgacatgggc attgccacct
       61 tcttgcttgg cagcatcggc atgcgaaagg gcctcctgcg catgcttggt ggcttcaccg
      121 gcatgcccca tttctccatg agcttttgcc tcaccggcat gcccgatcgc ttcatgcaaa
      181 tgtttatccc cttccgccaa tgtaacagcc ggaactgcca aaaagcccaa taccccaaca
      241 caaccgacaa ttcggatgaa tgtagagaat gtactgctca tgcctttcct cctttgatta
      301 ataacgtacc aaccatactg cagcctattt cctcacggtc ctcccggctc cgtcttctaa
      361 tacactgttt gtcataatcc attatgccat tcacaggaag aaacgtgaat gcataattct
      421 ttacccgatg aacaaagcca acggcaagct catataaggt ccttgcggca aaggcccctt
      481 atttacgacc gcgacacttt gacgaattga ccattgacca catacgaatt ttctgactat
      541 tcgatttttc gagatttttt attttcacat gaccacggac aaattttcta aacccattgg
      601 gaaatgtgtt gcatacatga gcaacgatct gtagacgatt tctgtggcta ttcctatact
      661 ctctccacgt caggtagcct ttttatgtga ttcagctatg atctgaccca taggaatcct
      721 gaccaatatt accacttgtt gtttttttgg atagaaggtg cgatagggaa ccctgacccc
      781 ctggaaaaac cttcttcctt ctgtggagaa cgatcgggat ggagcctggt atcgactacc
      841 tccgctcttc atcccgatct tcacggaacc tgaaatttgc acaccagaag atgtgattca
      901 cgccctgcct ttcatcatga aaacagggct tttctgcctg actggcagaa tattcccact
      961 cttgagaaaa atgaatttcc ccctgatcgg ggctccctgt caggtggaca atatcccctg
     1021 gctccgagcc ttccctcttc catttttctg attcgaattt tggcaaatca gtagacaata
     1081 cgatacaatt ttcaagattt gcttttctga atccgaccaa tggatcaaca cttcgactga
     1141 aagaaaatcc tcatgctcaa tccgagtcac ggcaattccc cgcatacttt ctcgccacgc
     1201 atgaagtggc aaagaatatt cctatgcctt atcttccttt ggcccttgcc agcgttgggg
     1261 caccaggcgg gaaaccttta tcgtgatgct ctttcagccg tggaaaatca ggacttcaag
     1321 accgcccaat ccttactcga acaggccatc caggaatttc ctggtttttc cgaagcccat
     1381 cacctgtatg gcttggtgga gtttcaactg acccaacaac ctgaacgcgc aattgctgcc
     1441 cttcaacagg ccatccgcct caacccgaat ttagctcaag ggcagtatga tttggcgtta
     1501 ctctttatca agcaagatca aatagaagaa gctcaaaaag ccgctcagca agcgctcgct
     1561 atctatccac gtttttggga ggcccgcctc atactcgcga aattattgga taagcgagaa
     1621 ctcacgaatc aggctattca ggaataccag acaattctca ctcaacaacc cttccaggaa
     1681 gaagcgctct atcatctagc tacccggttt atgcaagctc aagattttga ccaagcacag
     1741 ctcctcctta cccaattgac agaacaccac cctcaccaga cagacgcatg gttgttgctg
     1801 ggccggattg cggaacggca gaatcaaccc tcacaggcct tacatgctta ccaagaagtc
     1861 attcaggcca atcctgacaa ttcggaagcc cacgccaatc ttggatttct ttaccaacaa
     1921 caagggaacc taccagaggc cattgaacat ttccaacgcg tcagcgaact caagcctcag
     1981 gatgccgagg cctttctcaa tctcggagtc ctcttcgcag gaaaccatca gctcgtggag
     2041 gcagaacaag catatcaaac aggaatcgct ttgcaaccag attcactgga aggccatttc
     2101 aatcttggag ccttttacga attccacaag aaggatcttc ctcaagcacg aatccactac
     2161 cagaaatacc ttgaccttgg cggaaaagac gccagaatcc aacaattgct tacacaactt
     2221 gggcagtaga tcatccccat cggttcaagg aaatcctcga attcttcttc aagacctctc
     2281 caaccgctca atttgatatc cactccattt tttagtgctt ccatattctt cctcactagc
     2341 tcagaggctt ccctctttga tactttcagc ccctgaaacc ttattcgatg ctgttttcat
     2401 cttctccctc catgcctttt gattcacgcc tggaacccgc tgatcacatt taggctttgc
     2461 ttaagggcgg aatgccaacg tttttgtcca aaaagacctt cccgtaatgt tcgggaagga
     2521 cattctttcg atcacaacta caccaaaaat gaggaaaact agccatttgt agaacatttc
     2581 caatcggcaa gtttttcaat aatcagtcga tattctccta ccaaagaacc tccgcgttga
     2641 atcaacgaga aatgaatgag ttggtgtgag gtttgtgagg ttattgatcc ttttatccag
     2701 gagacacgga aaatgggacg caattcaatc aaagggttac agaatccgtt acgaaaccaa
     2761 caaggtgcat acatcctgat tacaggcatt gctgttgtgg ccctatttgg atttgcagcc
     2821 ctgggtattg aagttggacg gtggtatgcc atccagggag aaatgagtaa agcgatcgat
     2881 ggagccgcct ttgcgggagc caaaaatgtg aacaatccca atattaccga ccttcacggg
     2941 tttgtc
//
LOCUS       DAORAQ010000299         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4688_length_2945_cov_67.406920, whole genome shotgun sequence.
ACCESSION   DAORAQ010000299 DAORAQ010000000
VERSION     DAORAQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4688_length_2945_cov_67.406920"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..872)
                     /locus_tag="PKM72_14425"
     CDS             complement(114..872)
                     /locus_tag="PKM72_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNP62040.1"
                     /translation="MEHNDTIITQFTLQAVPFSELPGHSESMDMLVQFSGVCATDTVL
                     DVACGPGIVACEFAKHAAHVTGLDLTPTMIAEAGKRQQEQNLTNVSWHIGDVLPLPFP
                     DAHFSCVVTRYSFHHFLDPVAVLLEMIRVCKPGGRVMVVDAVLPSEKIDAYNRMEQIR
                     DPSHTKALSFDGMAAAIETSGLTNIKTGWYKVEMELEQQLAASFPHSGDKDKLRQMFQ
                     SDIGKDAMGVGVHRMGSEIRFAYPILITVGQKVA"
     gene            complement(964..1461)
                     /locus_tag="PKM72_14430"
     CDS             complement(964..1461)
                     /locus_tag="PKM72_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP62041.1"
                     /translation="MTIRELTSTDVSAYQTLMLSGLREHPESFRISSQDAGEPMVPFA
                     SICSDSFTLGAWLDEGQLAGAVSFARETQAKLRHKGLLYRMYVRAESSGRGIGRKLVQ
                     EVVKRARTIEGLEHINLTVVSLNVHAKSLYVSEGFTSFAREERGLKMGDSYFDEEQMA
                     LRLFK"
     gene            2040..2669
                     /locus_tag="PKM72_14435"
     CDS             2040..2669
                     /locus_tag="PKM72_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNP62042.1"
                     /translation="MKGSLLTYLLPIVMGLIVFNELSSHAQAKVIEPEESETVVLSGG
                     GGIFYPFQGKTGGSGAIQAMGIISPQERMGVELEYRNYKNDLLHAKNIDMQSFIFRGI
                     GQYFFRTHGISPYIGLGLNIALNVFDEKDIEKKRDSINVKGDKGFGYGVMGLVGVEAP
                     VAQGVALFAEGRVSADFQLTRYKNGSGKNKLDVENLSGLTGMIGIRFHF"
BASE COUNT          777 a          757 c          651 g          760 t
ORIGIN      
        1 gcaattaaaa gttgcattta aattttgcca ataactttag atttccaaaa tcattcctgc
       61 aaactcatga ctcatgtata actcccactc ttttccccgc tcagagaacc aggtcacgca
      121 accttttgtc caaccgtgat aaggatcggg taggcaaagc gaatctcact ccccatgcga
      181 tgcaccccaa cacccatggc atctttccca atgtcagact ggaacatctg gcgaagcttg
      241 tctttgtcac cagaatgggg gaaggaagca gccagttgct gttccaactc catctcgacc
      301 ttataccatc ccgttttgat attggtcaga cccgacgtct caatggctgc cgccatcccg
      361 tcaaaactaa gggctttagt atgcgaaggg tcccggattt gttccatccg attgtatgca
      421 tcgatttttt ctgatggaag aacagcgtca accaccatga ctcttccccc aggcttgcaa
      481 acccgaatca tctccaacaa cactgccacg ggatcgagga agtgatggaa gctatagcgc
      541 gtcaccacac acgaaaaatg ggcatcggga aatgggagtg gcaaaacgtc cccaatgtgc
      601 caggaaacat tggtgagatt ctgttcttgc tgccgtttcc ccgcttccgc aatcatggtt
      661 ggagtgagat caagaccggt cacatgagcg gcatgtttgg caaattcgca ggccacaatg
      721 ccgggaccgc aagccacatc gagaaccgta tcggtggcac acaccccact gaattgaacc
      781 agcatatcca tcgattcgga atggccggga agttctgaga aaggtacggc ctgtagggta
      841 aattgcgtaa tgatcgtgtc gttgtgttcc atagtttctt gctctcctaa acataccgac
      901 ctcatgccag gttcagggac aaacctttac tgacagatcg gctggaatgc cgtatgaggt
      961 tcatcacttg aacaagcgca aagccatttg ttcttcgtca aagtaggaat cccccatttt
     1021 taaaccgcgt tcttcacgtg cgaacgatgt gaagccttct gaaacataga gactcttcgc
     1081 atgaacgttg agcgaaacca ccgtcaaatt gatgtgttcc aggccttcga tggtccttgc
     1141 ccgcttcacg acttcctgga caagcttcct gccaattcct ctgccggagg actccgcacg
     1201 cacatacatt cgatacaaca agcctttatg tctcagcttt gcctgcgttt ctcgtgcaaa
     1261 actcaccgct ccagccaatt gcccttcatc aagccaggcc cccagggtaa aggaatctga
     1321 acagatcgac gcaaagggaa ccatgggttc tccggcgtct tgcgaactga tgcgaaagga
     1381 ttcaggatgc tctcgtaacc ccgaaagcat gagggtttga tacgcactca catccgtcga
     1441 tgttaactcg cgaatggtca tgctttcccc tcagacctcc tcgtgtttct ttggcgccaa
     1501 aaaatgaata gatatatcgg cataaggtgc agctctcgga ttttgggact gtattataga
     1561 ccatccaatt ctctggaaaa tacgcgatca tcggtcttat ttcacttcag ttgccttccc
     1621 aacggccatt gtttacagcc tcatctttcg acatccggta tcagatccgc ctagctcacc
     1681 tctcccttca attgggtaaa aataaatgcg gcgggattcc cggtgaattg ccaaaagcga
     1741 tcaactagtt gtccatgata agaacatttt ttccaaacaa gggaaggcgg agcattccac
     1801 aagcagtatc actgctagaa aatttaagtt tgtccatgcc ttccgccgat aaaaaactta
     1861 gcgattcctt ccctgctcat cagggacgtc aatgaccatc tcaataattt cctctcttcc
     1921 tgaaattgca tgacaagtcc ttcccttccg atatcaccag cacattctta tagtcttcat
     1981 cagaattttg tcattttctt tgcccattac agtgggcaaa tacaacccac cgggggaaca
     2041 tgaaaggatc gcttttaacg tatctgttac ccatcgtgat gggactgatc gtctttaacg
     2101 aactatcgag tcacgcacag gcaaaggtca tagaacctga ggaatcggag acggtggtgc
     2161 tttcgggtgg tggaggaatc ttttatccgt ttcaaggcaa aaccggggga agcggtgcca
     2221 ttcaagccat gggaatcatc tctcctcaag aacgaatggg tgtggagctg gaatatcgca
     2281 attacaaaaa cgaccttctt catgcgaaaa atatcgatat gcaatccttt attttcaggg
     2341 gaatagggca gtactttttc cgtacccacg gcatttcacc gtatatcggc cttggactca
     2401 atatcgcact caatgttttt gatgaaaagg atattgaaaa aaagagggat tcaattaacg
     2461 taaaaggaga caaaggtttt gggtacggcg tcatgggact cgtgggggtt gaggcccccg
     2521 tcgcacaggg agtggcccta tttgccgaag gccgggtgag tgcggacttt cagttaacgc
     2581 ggtataagaa tggatcagga aaaaataaac ttgatgttga aaatttaagt ggcctgaccg
     2641 gaatgatcgg cattcgtttt catttttgac cttcccatgc tatcgaacat tcacagcaaa
     2701 tagtcagata aaatggaaaa cgatccattc ttactacaga attcgtctgg tgccacagac
     2761 cccatacagt acatgaccga ctgcgcccgt cagacattcg gattgcagat ctcggctttc
     2821 tgctgatctg cccccaacta gtctaaaagt tccttcggaa tattgcctcc gttctcagcg
     2881 agtttttgca acacggtttt atgcaaccac atattcatct gggcggaatc ccccattttg
     2941 tcagc
//
LOCUS       DAORAQ010000300         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4777_length_2903_cov_63.930478, whole genome shotgun sequence.
ACCESSION   DAORAQ010000300 DAORAQ010000000
VERSION     DAORAQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_2903_cov_63.930478"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..574
                     /locus_tag="PKM72_14440"
     CDS             374..574
                     /locus_tag="PKM72_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62043.1"
                     /translation="MLPHTHSWYPIETVNWGKLSPTYPFRFNGPGYLYCMWCAHYIRL
                     VSVSKEGAKCSDCRRDLAVAIS"
     gene            complement(1077..1421)
                     /locus_tag="PKM72_14445"
     CDS             complement(1077..1421)
                     /locus_tag="PKM72_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP62044.1"
                     /translation="MLQVREKLNNQSTVIIFTGRFDRQSTIGIGDLILGSKDMRCQHI
                     ILDFMGITGIDSVGLGLLYLWYHKLQPQHIGLSIVGPSPNVQAALEGCHLPACVPVYA
                     TEHEAIRQEVYC"
     gene            complement(1763..2200)
                     /locus_tag="PKM72_14450"
     CDS             complement(1763..2200)
                     /locus_tag="PKM72_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNP62045.1"
                     /translation="MPALSESCLLPTKPLHSLLCLTDKPLMQDFDNSKHPTTRVLTLS
                     GHLDSLQTLRLEKFIEEAQQGTCRHVILNLSKVATMDLGGVGNLFTWYHALHINQVRL
                     SIVAPSPMVRNTMESLHLTELISIHSSIPEAISHHHVPNSVSS"
     gene            2312..2665
                     /locus_tag="PKM72_14455"
     CDS             2312..2665
                     /locus_tag="PKM72_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62046.1"
                     /translation="MALKRIRQAQGLSIRALAERAMAHYVNLAQVIDNHPFPQSIRSL
                     MGLIKIKKERYVKFRVVDNGKILRVAGETLGAKLKRWKTSTLNMTLSKKQRAKYNGLN
                     DGKNCERSSQRANDV"
     gene            2655..>2903
                     /locus_tag="PKM72_14460"
     CDS             2655..>2903
                     /locus_tag="PKM72_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62047.1"
                     /translation="MTFKALTDAYLTDPEFQRQRIFQKKSNWFSRRFLPVLGGSTLLD
                     KITEDKIEKYLEVRRFKLLLWLVPETGVEPVRAGKPEGF"
BASE COUNT          865 a          606 c          703 g          729 t
ORIGIN      
        1 ttcctcagcg aatgagatac gatctgtatc tattccgata cttcggcctc catggttaga
       61 tcgcaacttt ccttccccca cttttactgt ttcttttaat aaatcaataa gttgatagta
      121 gggcagccgg tcggtccgat gcgccggatt atatggcatt aaggttgcta atttatgagc
      181 aaagacgaga aaaaggacaa aaaagaaacc aggggggaca tttttatgga ctggacgacc
      241 ttatgggtaa tcgaaacggt gatagctttt ttgggagtct tttcccttca ctggttcttg
      301 aattagcaaa acctcacatt tcagcatagg ggtcggaacc aagaccattt tattgagaaa
      361 ggatttttag gtaatgctgc cacacacaca ctcatggtat cccatagaaa cggtcaattg
      421 ggggaagctc tcccccacct atccgtttcg attcaatgga ccagggtatc tctactgcat
      481 gtggtgcgca cattacattc gattggtatc ggtttcgaaa gaaggagcca agtgttccga
      541 ctgtcgacgt gacctagccg tggccattag ttaacttgag ccccttcaca gtagtttcta
      601 gttcttcatc agttttttct tcgtaggaaa aattttgggg aatatagaaa catctaatta
      661 agaaccattc agtccaatag tttcttgata ggggaaaaac cggccagcta cattttgatg
      721 gaagaaaaca aaaaaaccac acaacgaaat cacacaggga aagtaatacg cgaatgattc
      781 aattgtaagg gttacgactc aaggagaaat accactttgg catactcaaa aatccttact
      841 caaatctcct cgccacctga agaaatttgc cttcggtttc aatcacctct taagtcagtt
      901 gtggagcttt tcactaggat ttttgcagat caataccagg actcccagtg aattaaagta
      961 ggaggaaggc gacatattcc caaaaaaatg agagtttgat gactggtgtt cctataaccc
     1021 gggattagtg gatcaaagaa cagacgacca tactgatgtc cgaacgatga agccctctaa
     1081 cagtaaactt cctgtcggat tgcctcgtgc tcagtggcat agaccggaac gcaggcagga
     1141 aggtgacagc cttccagcgc agcttgaaca ttaggagaag gcccaacaat acttagccca
     1201 atgtgttgag gttgtagttt atggtaccaa agatacagga gaccgagacc caccgaatca
     1261 atcccggtga tgcccataaa atccaaaatg atgtgctgac acctcatgtc cttggatcca
     1321 agaatcagat cccctatacc aatggttgat tgtcgatcaa accgtcccgt gaagatgatg
     1381 acggttgatt ggttatttag tttttcgcgg acttggagca tcgaatctac ctcttaattt
     1441 gtaaagaata aaaatgagat gttaatacat agggctatat tcaatctttt ccgggtaggc
     1501 cgaacaatta ctcgttaaga ctagaggtac gtactggtgg tcctaatgag caaactactc
     1561 aaaaagtaaa gtgatgtcgt tgaactacca aaaagggtga gatcattcgt ggagtttccc
     1621 accgtgataa tgggtgaata ttgcagtttg cgacctgagc aacgaaagcc aagtgcaagg
     1681 gctaaaactc taaacttgct acgaaccagg tagagcgatg gattcagaaa tagacagtgc
     1741 tgccgatgac accaggaggt atctaggaag aaacggaatt ggggacatga tgatgactga
     1801 tcgcttcagg gatggacgaa tggattgaaa tgagttccgt caaatggaga gattccatcg
     1861 tattgcggac catgggggac ggagccacga tactgaggcg cacctgattg atatgcaaag
     1921 cgtggtacca cgtgaagagg ttgcccactc cgcccaggtc catcgtggcc actttggaaa
     1981 gattgaggat gacgtgacga caggttcctt gctgggcctc ctcaataaat ttttcaagcc
     2041 tcagggtttg aagggaatcc agatgtccgg aaagggttaa tactctggtg gtaggatgct
     2101 tggagttatc aaagtcctgc ataagaggtt tatcggtaag acagaggaga gagtgaagag
     2161 gctttgtggg taaaagacaa ctctcggaaa gtgcgggcat atttgaaccc accgcactgg
     2221 atggttatat caaaacgcaa atcgtgatgc cgcttccgag aaaactcagc taaccacttt
     2281 tagtctaaac actcacatga agaatccgga gatggcatta aagcgaattc ggcaagccca
     2341 agggctttcc attcgtgcat tggcagagcg ggccatggcg cattatgtca acctggccca
     2401 agtcattgat aatcacccat ttcctcaatc tataagaagt cttatgggac tgatcaaaat
     2461 aaaaaaagag aggtacgtga agtttcgtgt cgtggataac gggaagatac ttcgggtagc
     2521 aggagagaca ctgggagcca agctaaagcg gtggaaaacc tcaactctca acatgacttt
     2581 gtctaagaaa caaagggcca aatataacgg acttaatgat gggaaaaatt gtgagcgatc
     2641 aagccaaagg gccaatgacg tttaaggccc tcacagacgc ttatctgaca gaccccgaat
     2701 ttcaacgcca acggattttt caaaaaaagt ccaattggtt ttccaggcgg tttcttccgg
     2761 ttttgggcgg ctctactctt ctagacaaga ttactgaaga caagatcgag aaatatttag
     2821 aggttcgtcg cttcaagcta ttgctttggt tggtgccgga gaccggggtc gaaccggtac
     2881 gagctggtaa gcccgaggga ttt
//
LOCUS       DAORAQ010000301         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4809_length_2881_cov_71.762916, whole genome shotgun sequence.
ACCESSION   DAORAQ010000301 DAORAQ010000000
VERSION     DAORAQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_2881_cov_71.762916"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKM72_14465"
     CDS             <1..231
                     /locus_tag="PKM72_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62048.1"
                     /translation="QKDYSAEVVEIGPRVDPVSQTIRVFGRFHKHPTGVLAGMSGSAK
                     FPEGTFPTQSTFSPSTKPLASSTSMSKKTAKP"
     gene            280..1725
                     /locus_tag="PKM72_14470"
     CDS             280..1725
                     /locus_tag="PKM72_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNP62049.1"
                     /translation="MVQTATQTESPARGTGKDFRSSSGLSGGFSIAPKNKTEKDSSIF
                     LALLQLESQVRKAQTVKELCFLLANETRRLVDFRQACIFSITPKGRMRGRVEAVSSVA
                     VVDRNAPMITWIERVISELWGQGSLKDPGPLSSTHCSTELQRDWKSFAFPHVLWCPLI
                     RADQQVIGGLWLTREIPWQDGEVQLIRRFAETVAHAWQALGGTKRRVKSWKVLKNWLW
                     GVLVVVMAAMWLPVRLSTLAPVEVVPRDPSVVTAPLDGVLGEILVHPNTLVQTGQTLF
                     RYEDTTFRNQFEVAEKNLSVTWMEYRKVSQGAFVDHDTGAGMPVQASEVRLKEAERDY
                     AWDMLQQVEVKAPRAGIVIFTDKSEWIGKPVVVGERIMEVADPQEFELKIDLPVEDAI
                     VLREGAEVEVFLNAHPLRAIPAQLIHASYHASILPTNVLAYRVEAEFTDPPEDIRIGW
                     QGTAKIYGEPVTLFFYLFRRPLGVLRQTLGF"
     gene            1725..>2881
                     /locus_tag="PKM72_14475"
     CDS             1725..>2881
                     /locus_tag="PKM72_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNP62050.1"
                     /translation="MGQPKPQEDISLPPLREDLQILPGTPTPDGVPTWTIVDMVRNQF
                     FQIEWSAFQLLSHWQAGTAKGLVDLVTRTTSAKVSTGNVMELVQFLYRNNLTRDSLAG
                     GSQGFLGQAEQAKQHWLLRVLHNYLFFRIPLCNPDRFLRTTLPFVIPLYSRCALWCVV
                     ILGVIGCMGVVRQWESFANVFLYFFTFQGMTGYIIGLVAIKILHELGHAYTAVRYGCR
                     VPTMGLGFLIMVPVLYTDTTDSWRLTVRKQRAAIAAAGMVVELSVAMLATFLWNFCPD
                     GIVKSMLFVLATTSWVTGLLINLNPLLRFDGYYVLSDWLGVPNLQSRAFGFGRWKLRE
                     WLFAWGDAPPEHMPPQRQSVLIAYAWAVWVYRAVVFVGIAVLVYYFFFKVLG"
BASE COUNT          607 a          733 c          825 g          716 t
ORIGIN      
        1 caaaaggact attcggcgga agtggtggaa attggtcctc gtgttgatcc tgtgagccaa
       61 accattcggg tctttggccg gtttcacaaa catcccactg gtgtcttagc cgggatgagt
      121 ggaagcgcca aatttccgga aggcacgttt cccactcaat caacattttc gccaagcacg
      181 aaacccttag cctcatcgac gtccatgagt aaaaaaactg ccaaacctta actccttccc
      241 ggatcaacct ccctgttgat ccaacggaga gaaacgagcg tggtgcaaac agcaacccag
      301 acagagtccc ctgcgcgagg gaccgggaag gacttcagat cctcctccgg tctgtccggc
      361 ggcttttcca tcgctcccaa gaacaaaacg gaaaaagatt ccagtatttt tctggcgctt
      421 cttcaactgg aaagtcaggt tcgcaaggct caaacggtaa aagaactctg ttttcttctg
      481 gccaatgaga ccagacgtct cgtggacttt cgacaggcct gtattttttc catcacgccg
      541 aaggggcgga tgcggggaag ggtggaagcg gtgtccagtg tggccgtggt tgaccgcaat
      601 gcgccgatga tcacttggat tgaacgcgtc atttccgaat tatggggaca aggcagtctc
      661 aaagatcccg gtcctctctc ctccactcat tgttcgacag aactccaacg tgattggaag
      721 tcctttgcat ttccgcatgt cctctggtgt ccattaatcc gcgcagatca gcaggtgatt
      781 ggtggcctgt ggttgaccag ggagataccc tggcaggatg gggaggtgca attgatccgg
      841 cggtttgccg aaacggtggc tcatgcctgg caggcgctcg ggggcacgaa acggagagtg
      901 aaaagctgga aggttcttaa gaactggcta tggggagtgc tggtggtcgt gatggcggct
      961 atgtggctgc ccgtgcgatt gtccacgctg gctcctgtgg aagtggtgcc gcgggatccg
     1021 tctgtcgtga cggccccatt ggatggggtg ctgggagaaa tccttgtcca cccgaatacc
     1081 cttgttcaaa cagggcaaac cctctttcgc tacgaggaca ccacattccg gaatcaattt
     1141 gaagtggcgg aaaagaatct gtccgtcacc tggatggagt atcgcaaagt gtcgcagggt
     1201 gcatttgtcg accatgacac cggtgcgggt atgcccgtgc aggcctcgga agtccggctg
     1261 aaagaggccg aacgggatta cgcctgggac atgttacagc aggtggaggt caaagctccc
     1321 cgggcgggca ttgtgatttt cacggataaa tccgaatgga tcggcaagcc ggtggtggtg
     1381 ggcgaacgca ttatggaagt ggccgatccc caggagtttg aattgaaaat tgatctaccg
     1441 gtggaagacg ccatcgtgtt acgggaaggc gccgaggttg aggtgttttt gaatgcccat
     1501 cccttacgag ccatacctgc ccaattaatt catgccagtt accatgcgag tattctcccg
     1561 accaatgtcc tggcctatcg ggtggaagcc gagttcacgg atccgcctga agatattcga
     1621 attggctggc aaggcaccgc aaaaatctat ggggaaccgg tcaccctctt tttctatctc
     1681 ttccggcgcc cactgggagt tcttcgacaa acgttaggtt tctaatgggt cagccaaagc
     1741 ctcaagaaga catttccctt ccacccttgc gcgaagatct tcagatcctg cccgggaccc
     1801 ccacgcctga tggcgtcccc acctggacga ttgtggacat ggtgcgcaat caattttttc
     1861 aaattgaatg gtccgccttt caactgcttt cccattggca ggccggaacg gcaaagggac
     1921 tggtggatct ggtgacccga accacctcgg ccaaggtctc aaccgggaat gtgatggagt
     1981 tggtccagtt tctttatagg aacaatttaa cccgggattc cttagccggt ggaagtcagg
     2041 ggttcttagg acaggccgaa caggcgaaac aacattggct gctgagagta ctccacaact
     2101 atttattttt cagaattccc ctatgtaatc ccgaccggtt cttacgaaca acgcttccgt
     2161 tcgtcattcc gctgtatagt cgctgcgcct tatggtgtgt ggtcatcctg ggtgtgatcg
     2221 ggtgtatggg agtcgtgcgg cagtgggaat cgtttgccaa cgttttttta tactttttca
     2281 cgtttcaagg gatgaccggt tatatcatcg ggttggtggc cattaagata ttgcatgaac
     2341 tggggcatgc ctataccgcc gttcgatacg ggtgccgggt ccccacgatg ggcctcggat
     2401 tcctcattat ggtgcctgtc ctctataccg atacaaccga ttcctggcgg ctcacggtcc
     2461 ggaaacaacg cgcggcgatt gccgctgccg gaatggtggt tgaactgagt gtggcgatgc
     2521 tcgcgacctt tttatggaac ttctgtcctg atggcattgt caaaagcatg ctctttgtgt
     2581 tggcgaccac cagttgggtg acggggctgc tgatcaattt gaatccgctg ctccggtttg
     2641 acggctacta cgtcctctcc gattggctgg gtgtcccgaa tttgcaatca cgggccttcg
     2701 ggtttggtcg ttggaaacta cgggaatggt tgtttgcctg gggagatgct cctccggaac
     2761 acatgccccc gcaacggcaa tcggtgctga tcgcctatgc gtgggcggtg tgggtctacc
     2821 gggccgtcgt gtttgtcggc attgcggtcc tggtgtatta cttttttttc aaggttttgg
     2881 g
//
LOCUS       DAORAQ010000302         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4854_length_2859_cov_62.115906, whole genome shotgun sequence.
ACCESSION   DAORAQ010000302 DAORAQ010000000
VERSION     DAORAQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4854_length_2859_cov_62.115906"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..487
                     /locus_tag="PKM72_14480"
     CDS             38..487
                     /locus_tag="PKM72_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62051.1"
                     /translation="MGGFRSGPLIFMVFGVAPIFLFASPTPALPCESLLNPLAFQVTT
                     FTQNTLLPNGMMAIRYPATEQEVSPHVSVHRILMVIPGLQESLPEASDIHYRIVEGDG
                     TIGWITYIVSAHALFYGVGEDSLGFPEKMWIDPEEDGLNGNEHINGR"
     gene            complement(542..766)
                     /locus_tag="PKM72_14485"
     CDS             complement(542..766)
                     /locus_tag="PKM72_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNP62052.1"
                     /translation="MPIFAYKCQGCSHHFEMLVHSSTIPECPKCQANTLEKQLTGFAV
                     GGTGSGMNIVDPGSCGSCGDPRGPGACSMN"
     gene            833..1147
                     /locus_tag="PKM72_14490"
     CDS             833..1147
                     /locus_tag="PKM72_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62053.1"
                     /translation="MEDREGSGFTVMMEKEKKMYQEEESFNLRFSLEANFPDEYEGEE
                     DERAWLAHWEEKVKPDIVKGIFRTLEQYPDWMARFRNRGMAATDEIEIVLEKTYTDST
                     LN"
     gene            1212..2660
                     /locus_tag="PKM72_14495"
     CDS             1212..2660
                     /locus_tag="PKM72_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNP62054.1"
                     /translation="MAGGPAYAQNLIYSPTPGNPVGRAPQAITAGDFNGDGLWDMATV
                     NSTSDDVSVLLGNGNGTFQSAVSFGVGKIPLAVVAADMDGDGRLDLVLALTGSDQVVV
                     LKGEGTGMFQKLASQGAGKGTTFLAVRDLNGDGWSDVVAVNSGRFGYYPPFDLAVLLN
                     DGKGGLLAPVTYEQNGRDGMFPTGVLVEDLTGDGKPDLAVTWSQPSWRSPNGLVSILK
                     NTGNGTFALEKELKSGFTLSAVQGADINHDGLVDLAVTSLFSDSVRILLQRNGGTFTE
                     LDPIKVGFAPVGVTFRDFNGDQEIDMVVVNRDSNSLSILLGDGAGLFTSAGHFGVGAA
                     PSAMVVEDFDQDQFPDLATASTNADGVSVLLSGDAAIPLPSLSTDVLVYEMESGQETD
                     PSSSTHVVRVSNIGLGLLKIVDVKITGNEADAFSVVENTCADVTLHTGDSCMLNVGFA
                     LHEPGTHYAMLMVHDNASGSPRRVVLKALVKS"
BASE COUNT          649 a          657 c          808 g          745 t
ORIGIN      
        1 aaagtcgtcg tgaagattcc cgagaaggag gtgtcctatg ggaggcttca gatctggtcc
       61 acttatcttt atggtctttg gggttgcccc cattttcctg tttgcctccc ccactcccgc
      121 gttgccgtgt gaatctctac tcaatcctct ggcgtttcag gttacgacct tcacccaaaa
      181 caccctgctt cccaatggca tgatggccat tcggtatccg gcaactgagc aggaagtttc
      241 tccgcatgtc agtgtgcatc gaatattgat ggtgattccg ggattacagg aatcacttcc
      301 ggaagcctct gatatacact atcgaattgt tgagggagat ggcacgatcg gctggataac
      361 ctatatcgtt tccgcccatg cattgttcta tggcgtgggt gaggattcct taggctttcc
      421 ggaaaagatg tggattgatc ccgaagagga tgggctgaac ggcaacgaac acatcaatgg
      481 caggtaaact gattcaggga aatgaggcgt catacgcctc cagggtagat tgccagcccg
      541 tttaattcat cgagcaggcg ccgggtcccc ggggatctcc acaggatccg caactgcccg
      601 gatcaacaat attcattcca ctacctgtcc cgcctacggc aaatcctgtc agttgttttt
      661 ctaacgtgtt ggcctgacac tttggacatt ccggaattgt cgagctatgg acaagcattt
      721 caaaatgatg cgaacatcct tgacacttat aggcaaatat cggcatgggt tctggacctc
      781 cttttcaatg aagtggtaga tctattatag gcgaccacat gagataagga caatggagga
      841 tcgcgaaggg agcgggttta cggtcatgat ggagaaggag aaaaagatgt atcaagaaga
      901 ggaatcattt aatctgcggt tttccctgga agccaatttc ccggatgaat atgagggcga
      961 ggaagatgaa agggcgtggc ttgcccattg ggaagaaaaa gtgaagcctg atatcgtgaa
     1021 aggtatattt cgcacattgg aacagtaccc tgactggatg gcacgttttc gcaatcgagg
     1081 gatggcggcc acggacgaga ttgaaattgt cttagaaaaa acctataccg attctacatt
     1141 gaattaaccg tattatgatc tttaggccat tcgtaaaacg gtatggaatc ttagttattg
     1201 cctgggtttg tttggctgga ggtcctgcct atgcccagaa tcttatctat tcccccacac
     1261 cgggcaatcc ggttggacgc gctccgcaag ccatcacagc gggagatttc aatggagacg
     1321 gattgtggga tatggcgacg gttaacagca cctccgacga cgtctcagtg ctcttaggca
     1381 acggcaatgg cacctttcaa tctgcggtat cctttggagt cgggaaaatt ccgctggcgg
     1441 tggtggccgc cgatatggat ggcgacggcc gtctcgactt ggtcctggcg ttaaccggct
     1501 ctgatcaggt ggttgtgctg aaaggtgagg gcacaggaat gtttcagaag ttggccagcc
     1561 agggggcagg caaggggacg acctttttag cggtccggga tcttaacggg gatgggtgga
     1621 gtgatgtggt ggcagtgaat agcggacgat ttggttacta cccgcctttt gatcttgcgg
     1681 tgctcttaaa tgatggcaag ggaggcctgt tggcccccgt gacttatgaa cagaacgggc
     1741 gtgacggaat gtttcctacc ggtgtgctgg tcgaggatct gacaggcgat ggaaaaccgg
     1801 atcttgcggt aacatggagt cagccgagtt ggcgttctcc caacgggctt gtctccatac
     1861 tgaagaatac cgggaatggg acatttgccc ttgagaagga actcaagtct ggttttacct
     1921 taagcgcggt tcagggggcc gacatcaacc atgatggatt ggttgacctt gccgtgacca
     1981 gcctcttttc ggacagcgtt cgtattctgc tacagcgaaa tggaggaacg tttaccgaat
     2041 tggatccaat taaagttggc tttgccccgg tgggcgtgac gtttcgggat tttaatgggg
     2101 atcaagaaat agacatggtt gtggtcaatc gtgattcaaa tagtctgtcg attttgttgg
     2161 gggatggagc cgggcttttt acctctgcgg gacactttgg tgttggagcc gcgccgtcag
     2221 ccatggttgt ggaggatttt gatcaagacc aatttcccga tttggccacg gccagcacca
     2281 atgccgatgg cgtttcggtc ctgcttagcg gggatgcggc tatccccctc ccaagcctga
     2341 gcaccgatgt cctggtctat gagatggagt cgggccagga gacggatccc tcctcctcta
     2401 ctcatgtggt gcgggtctcc aacatcggat tgggacttct caaaattgtg gatgtgaaga
     2461 tcaccggcaa tgaagcggat gccttttctg ttgtggagaa cacttgcgcg gatgtcacgt
     2521 tgcatacggg cgattcgtgc atgctcaatg tcggattcgc tctacatgag ccgggaaccc
     2581 actatgccat gcttatggtt cacgataatg ccagcggtag tccgcgtcgt gtggtgttaa
     2641 aagccctggt gaaaagttaa gaaggtgctc tcctgagcat gaatgtgaat ctgagtcagc
     2701 cattttttga acctcgcttc atcccgccat ttgaattctg cgcttcaaac ctttcccaaa
     2761 atccggtatg ctcttcttcc gtttcctgaa gcagagtgac tttcatgtcg acttccggtc
     2821 aatccagtca aaatgtgtgg gtgggtatcg atattgggg
//
LOCUS       DAORAQ010000303         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4942_length_2817_cov_74.291818, whole genome shotgun sequence.
ACCESSION   DAORAQ010000303 DAORAQ010000000
VERSION     DAORAQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4942_length_2817_cov_74.291818"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKM72_14500"
     CDS             <1..911
                     /locus_tag="PKM72_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNP62055.1"
                     /translation="EILQTSPLITQFKFGTRTADTALNMRDGETVVLGGLLSEDDTKT
                     RDSVPGVDDIPGLGDLLSNTNKEKITTEVILVITPHIVRNVQPQQLAKQTLWSGTANQ
                     YTTKPLFSQPNPAVALMNTQDFPTEMTNSSDSSAFPQNPQFTEGKTPTQESVEQDTAE
                     ASQPMADEPHLQILPSAASIKMGEEISVTLQGENFSSTGNSSLTLSYDPAVVTFKNAV
                     EGPFWSNQSVPSSLTVSVVPNIGKIVLQMGQQGKSVQGSGSLATVVFEAAGAGNSNID
                     IQQTTVLGANGQPIPVMVQHGRILVE"
     gene            889..1428
                     /locus_tag="PKM72_14505"
     CDS             889..1428
                     /locus_tag="PKM72_14505"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNP62056.1"
                     /translation="MAEFWWNKSSPTQGGVTLIELLVTLVIMFILASVALPIAKISIK
                     RSQELELRHTLRTVRSAIDAFHLDWARDGTVRTGKLCVENVTSCQEATGATGYPKTLE
                     TLLKVKLTGEKAQLGEESEIKRYLRKIPFDPITESTEWGLRCFQDEPDETRWCGEDVF
                     DIYTTSEKKAIDGTPYREW"
     gene            1428..1829
                     /locus_tag="PKM72_14510"
     CDS             1428..1829
                     /locus_tag="PKM72_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNP62057.1"
                     /translation="MTHFVVIGKKGYTLLELMIVVAIVGILVSIAIPTFQHTAIKAKE
                     AALKQNLFTMRAVIDQFYADRKEYPATLEDLVEEKYLRAIPVDPFTRSSTTWSEIYEE
                     QEEGEDSPAGIFDIKSGSNGQALDGTPYTEW"
     gene            1837..2766
                     /locus_tag="PKM72_14515"
     CDS             1837..2766
                     /locus_tag="PKM72_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNP62058.1"
                     /translation="MKRNSWTHMVLSGTFILALGGCTQKLTKPVAVDSSIGHASQPSQ
                     HQILLQEARTENENLRAEIGAIKVLMAKQAGELQSLRGHTQSIQHREQDQGVELQHLR
                     GELLNSQAERDELRRHNMELEGRVAGIPDTTQLMADIQTLNQSFHQIMTSIKQLSTDV
                     TLIKQQIHKPSGKGQPLQTKFPISQPPVSSGDKRMPDSQGQIVIQSGDSLWKLSEMYQ
                     VSIAQLREWNDLDSDVIMTGLKIQVTSPTVPQLKSVNTSSSPSEVPTSPAIQEPLTEP
                     VHETIPLKDNRVESVPEPKHILSLGNPHSPESP"
BASE COUNT          802 a          727 c          664 g          624 t
ORIGIN      
        1 gggaaatact tcaaacatct cccttaatta cccaattcaa gtttggaacc cgtactgcgg
       61 acaccgcact caatatgcgg gatggtgaga ccgtcgtgct cggcgggctc ctttcagagg
      121 atgacacaaa gacgcgggat agcgttcccg gggtcgatga cattcccggc cttggtgacc
      181 tcctcagtaa caccaataag gaaaaaatta cgactgaagt cattttggtg attacccccc
      241 atatcgtccg aaacgtccaa cctcagcagt tagcgaaaca gactctctgg tccgggacgg
      301 ccaatcagta caccaccaag ccgctgtttt cacaaccaaa cccggctgtg gcccttatga
      361 acacccagga cttccctaca gaaatgacca attcttcgga ctcgtccgca tttccgcaaa
      421 atccgcaatt cactgagggc aagacgccca cccaagaatc tgtagaacag gatacggcag
      481 aggcatctca gccgatggcc gatgaacctc accttcaaat actaccgtca gccgcatcga
      541 ttaaaatggg ggaagaaatt tccgttactc ttcaagggga aaatttctcc agtaccggaa
      601 attcgtctct cacgctatcg tatgatccgg ccgttgtgac ttttaaaaat gcagtggaag
      661 gtcccttctg gagcaaccag tcggttccct ccagtctcac ggtttctgtc gtccccaata
      721 ttggcaaaat cgtgctccaa atggggcaac aaggaaagtc tgtacaagga agtggctcgc
      781 tagccacggt ggtattcgaa gccgcaggag ctgggaattc aaacattgac attcaacaaa
      841 caacggtctt gggagccaat ggccaaccta tcccggtcat ggtccaacat ggcagaattt
      901 tggtggaata aatcctcgcc aactcaaggc ggggtaacct taatcgaact attagtcacg
      961 ttggtcatta tgtttatatt agcctccgtc gccctaccca ttgcgaaaat cagcattaaa
     1021 cgatcgcaag agttagagct gcgccatacc ttacgaacag tacgaagcgc cattgatgcg
     1081 tttcatctgg attgggcgag agatgggacc gttcgtacag ggaagctctg cgtggaaaac
     1141 gtgacgtcat gtcaggaggc caccggagcg accggctatc ctaagacgct ggaaacactc
     1201 ttgaaggtta aattaacagg ggagaaagcc caactgggtg aggaatcgga aattaaacgg
     1261 tacctccgga aaatcccatt tgatccaata acagagtcca ccgaatgggg gctccggtgt
     1321 ttccaggatg aaccagacga aacgcggtgg tgcggggaag atgtgtttga catctatacc
     1381 accagcgaaa aaaaggcaat tgatgggacc ccctatcgtg aatggtaatg acccactttg
     1441 tagttattgg caaaaagggc tacactttac ttgaactcat gattgttgtg gcaatcgtgg
     1501 ggattctcgt ttccatcgcg attcccacgt ttcaacacac ggcaatcaaa gccaaggaag
     1561 cggctctgaa acaaaattta ttcacaatgc gagcggtcat cgatcaattt tatgcggata
     1621 gaaaggaata tcccgcaacc ttggaggacc tggtggaaga gaagtacctt cgagccatcc
     1681 cggtagatcc atttaccagg tcctcgacca catggtccga aatttatgaa gaacaggagg
     1741 agggcgagga ctctcctgct ggaatttttg atattaaaag tgggagtaac ggccaggcgc
     1801 tggatggcac tccctataca gaatggtagg tagcatatga aacgtaatag ttggacacac
     1861 atggtactga gcggcacatt tatcctggcc ttaggcgggt gtactcagaa attgacgaaa
     1921 ccggtcgcgg tcgattcatc aatagggcat gcctcccagc cttctcaaca tcagattctc
     1981 cttcaggagg ctagaaccga aaacgaaaat ttacgggctg agattggggc aataaaagtt
     2041 ctcatggcca aacaagccgg tgaattgcaa tcgctacgag gccatactca atccattcag
     2101 catcgagaac aggatcaagg ggttgaactg caacaccttc gtggagaact tctgaactct
     2161 caagcggagc gggatgaact gcgaagacac aacatggaac ttgaaggccg ggttgccggt
     2221 attccggaca ccacgcaact catggcagac attcagaccc tcaaccaatc ttttcaccag
     2281 atcatgacaa gcataaaaca gctttccact gatgtaactc ttattaaaca acaaatccac
     2341 aagccctcag gtaagggaca gcctcttcaa acgaaattcc caatctccca accaccggtc
     2401 tcttccggag ataaacgcat gccagattca cagggacaga tcgtcattca aagtggcgac
     2461 tcactctgga aactctcgga aatgtatcaa gtctccatag cccaattacg cgaatggaat
     2521 gatctcgatt ctgatgtaat tatgaccggg ttgaaaattc aggtcacctc cccgacagtc
     2581 ccccagttga aatcggtaaa tacctcgagt agtccaagtg aggtgccgac ttcccctgct
     2641 atccaagagc ccctgactga accggtacat gaaaccattc ccctaaagga caatcgtgtg
     2701 gaaagtgtcc ctgagccaaa acatatactc tcgcttggca atccccattc gcctgagtca
     2761 ccctagtgcc ccggtttcat tacaaagccg ctcgtccgga tggaacgatt attgaaa
//
LOCUS       DAORAQ010000304         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4983_length_2798_cov_66.446591, whole genome shotgun sequence.
ACCESSION   DAORAQ010000304 DAORAQ010000000
VERSION     DAORAQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4983_length_2798_cov_66.446591"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PKM72_14520"
     CDS             <1..398
                     /locus_tag="PKM72_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP62059.1"
                     /translation="PSVMTAIGGIAVLSRYVRSQMLEVLSQDYVRTARAKGLQEDTVI
                     YGHALGNALLPFVTMFGLLLPGLIGGSVIFEQIFAWPGLGRLAYEAILSRDFPIIMTL
                     NFIAAVLTLLGTLISDILYAVVDPRIRLH"
     gene            427..1419
                     /locus_tag="PKM72_14525"
     CDS             427..1419
                     /locus_tag="PKM72_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNP62060.1"
                     /translation="MNHSKTGASLKTKMASLDAEGLAPQETPWEEFLRVFTKDYQALF
                     GFWVLVLLLLMAVSGKVLTEWWVVFDPEAVRLTDKFLPPLSVPSPDSIPEDRPFGGVY
                     LMGTDELGRDVFARMFQGSFVSLSIGFVAVGISLGIGILLGGLSGFYGHVKLWFITVD
                     TLIMRFTDAMMCFPTFFLILTAVALLPPSIYTIMIIIGLFSWMGTARFVRAEFLSIRE
                     QDYVTAAKALGIPEGRIIFRHMVPNALAPVFVSATIGVASAILTESGLSFLGFGVPPP
                     YATWGNILSDGKGFVFDAPWLFFIPGLAIFLVVLAFNLVGEGLREALNPKLRSR"
     gene            1420..2406
                     /locus_tag="PKM72_14530"
     CDS             1420..2406
                     /locus_tag="PKM72_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP62061.1"
                     /translation="MESRVHPLLQVSNLRTSFFTDKGEIKAVDDVSFSIRGGQTLALV
                     GESGCGKSVAALSIMRLISAPGRVIGGTIHFKGQNLLDLPEKDMRRIRGKSIGMVFQE
                     PMTSLNPVMSIGDQIGEVLKIHTNLSDRDIRKEVISLLEKVRISAPESRIDQYPHEMS
                     GGMKQRVMIAMALACKPDLLIADEPTTALDVTIQAQILDLLSALQKDLGMAILLITHN
                     LGVVAQFAQDVIVMYASKIAERATVKELFRKPSHPYTSALLKSLPRPGERQARLEAIP
                     GTVPSPLQYPPGCHFSSRCPEVMEHCPLQPPPTVQVGESHETVCWLYDKKET"
     gene            2479..>2798
                     /locus_tag="PKM72_14535"
     CDS             2479..>2798
                     /locus_tag="PKM72_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNP62062.1"
                     /translation="MTDSTKSLLQVTGLKKYFPVRSGLFSRVSAWVQAVDDVTFHLNQ
                     GETLGLVGESGCGKTTVGRSILRLMEPTAGTVTFEGKDVLALSSKELRQTRRRMQIIF
                     QDPY"
BASE COUNT          593 a          682 c          775 g          748 t
ORIGIN      
        1 ttccatccgt catgacggca atcgggggga tcgcggtgct gtctcgctat gttcggtctc
       61 agatgttaga ggtattgagc caggattatg tccggaccgc cagggcgaaa ggtcttcagg
      121 aggataccgt gatatatggc catgcgttgg ggaatgcgct actgccgttt gtgaccatgt
      181 tcggtcttct gctccccggg ttgattggcg ggtccgtgat cttcgagcag atttttgcct
      241 ggccggggtt gggaaggtta gcctatgaag ccattctttc aagagatttc ccgatcatca
      301 tgacactcaa ctttatcgcc gcagttctga ccctcttggg caccttgatc tcggatattc
      361 tgtatgcggt ggtggatccg aggatccgcc tgcattaatc acgagcacca gagtaacgat
      421 cccttcatga atcactcaaa gaccggcgct tcacttaaaa ccaagatggc ttcccttgat
      481 gcggaaggcc tggctccaca ggaaacgcca tgggaagaat tcctccgcgt atttaccaag
      541 gactaccagg ctctcttcgg tttttgggtc cttgtgcttc tcttgctgat ggcggtatcc
      601 gggaaagttc tgacagaatg gtgggtcgta ttcgatccgg aagcggtgcg actgacagat
      661 aaatttcttc ccccgttgtc tgtgccttct ccggatagta ttccggaaga tcggcccttt
      721 ggcggggtct atctcatggg caccgacgaa ttggggcgtg acgtgtttgc ccggatgttt
      781 cagggctcgt ttgtatcgct gtctatcggg tttgtggccg tcggaatttc cttggggatt
      841 ggcatcttgt tgggcggact ttccggtttt tacgggcacg tgaagttgtg gtttattacc
      901 gtcgatacgc tgatcatgcg attcaccgat gccatgatgt gtttcccgac atttttcctc
      961 atactgactg ccgtggctct cctcccgccc agcatctaca ccatcatgat cattattgga
     1021 ttgtttagtt ggatggggac ggcccgattt gtccgggcgg agtttttgtc tatccgtgag
     1081 caggactatg tgaccgcagc taaagcatta gggatacccg aggggcggat tattttccgg
     1141 catatggtgc ccaatgctct ggcgccggtg tttgtgtcag ccacaatcgg cgtcgcctcg
     1201 gcaattttaa cagagtcggg tttaagcttt ctgggttttg gagtacctcc gccctatgcc
     1261 acatggggca atatcctgtc ggatggcaaa gggtttgtgt tcgatgcgcc ctggttattc
     1321 tttattcccg gcctggcgat ttttttggtg gtgctggcct ttaatcttgt tggagaaggc
     1381 ctgcgtgagg ccttaaatcc gaaattgcga agccggtaaa tggaatcccg cgtacaccca
     1441 ctattgcagg tgtccaacct tcggacctcg tttttcacgg ataaaggtga aatcaaagcc
     1501 gttgatgatg tgagcttttc cattcgaggc gggcaaacgc tggccttggt gggagaatcc
     1561 ggctgtggga agtccgtggc agccctgtcg atcatgcgtc tgatctctgc tcctggtcgt
     1621 gtgattggtg gcacgattca tttcaaaggg cagaatttgc tggatctacc cgaaaaggac
     1681 atgcgccgga ttcgtggaaa atccattggc atggtgtttc aagagcccat gacctcgctg
     1741 aatccagtca tgtcgatcgg cgatcagatc ggtgaagtcc tgaaaatcca tacgaaccta
     1801 tcggatcgcg atattcgaaa agaagtgatc agcttgttgg agaaagtgcg tatttctgcg
     1861 cctgaaagcc gtatcgatca atacccccat gaaatgtccg gcgggatgaa gcagcgggtg
     1921 atgatcgcca tggccctggc ctgtaaacct gacctgttga ttgccgacga accgaccacg
     1981 gccttggatg tgaccataca agcgcaaatt ctggatctgc tgtcggctct tcagaaggat
     2041 ttgggtatgg ctattttgct gatcacacat aacttgggcg tggtcgcgca atttgcccag
     2101 gatgtcattg tcatgtatgc gagcaaaatt gccgaacggg caaccgtgaa agaattattc
     2161 cgcaagccca gccatcccta cacgagcgcg ctcttgaaat cgctgccgcg accgggtgaa
     2221 cgtcaggcca gattagaagc cattccggga accgtgccct ctcccttaca gtatccgccc
     2281 ggttgccatt tttcttcccg ttgtccggaa gtgatggagc attgccctct tcagcctccg
     2341 ccaacagtgc aagtggggga gtcgcatgaa accgtgtgtt ggttatatga caaaaaagag
     2401 acatgatgaa aacgaactgc atgactattc ctgttgtaaa aagattctac atcccacttt
     2461 aaaaattcgt cgttactgat gactgattcc acgaaatcat tactccaagt tacagggttg
     2521 aaaaaatatt tccccgtccg aagcggactc ttttcccgtg tgtcggcatg ggtccaggcg
     2581 gttgacgatg tcacctttca cttgaatcaa ggggaaacgt taggcctggt cggagagtcg
     2641 ggttgtggaa aaaccacggt gggacgaagt attctcaggc tcatggaacc gacggcggga
     2701 acggtaacct tcgagggaaa agatgtgttg gccctctctt cgaaggagtt acggcagaca
     2761 cgtcgccgga tgcaaatcat ttttcaggac ccgtatag
//
LOCUS       DAORAQ010000305         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_4999_length_2791_cov_69.654605, whole genome shotgun sequence.
ACCESSION   DAORAQ010000305 DAORAQ010000000
VERSION     DAORAQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4999_length_2791_cov_69.654605"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..276)
                     /locus_tag="PKM72_14540"
     CDS             complement(31..276)
                     /locus_tag="PKM72_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62063.1"
                     /translation="MKKIKINLLIGLMTILSSVSCASDPVKWYRSGTSQETFTRDKSD
                     CEEALLSTGSTQREKDIYSLEGCLESKGYTAIPLSSQ"
     gene            complement(434..1207)
                     /locus_tag="PKM72_14545"
     CDS             complement(434..1207)
                     /locus_tag="PKM72_14545"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNP62064.1"
                     /translation="MLPFPTSPAILIGAICFYGLIGCVIESPPEPPETVTERLIPLLT
                     DTQPDIRRTAALSLGKIGDPQSVPALVLALSDPDDQVRQSSAWALGTMAESLSDQSLV
                     ALVQHLTDPSDSVKKAVVLALGRTAVHEDLLQVLTEAYAISTIDTQRTIIQSLAHFEF
                     PFSYSVYLQALESPDSLTRQSAIAGLGELGDPRGLPYLRTHLLQDPSVGVRSEAAFRL
                     GKLGTSADVFALKQAMETDPTPNVHFWSSWALVQIGPNT"
     gene            complement(1215..2003)
                     /gene="tatA"
                     /locus_tag="PKM72_14550"
     CDS             complement(1215..2003)
                     /gene="tatA"
                     /locus_tag="PKM72_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNP62065.1"
                     /translation="MFGTMGFSELMIILVIILIIFGAGRLPQIGEGVGKALKGFKKEV
                     ADIPQPVDPNEPLPSAPPEPAQAQSQPTTPVGTQTNQPFQPGPEQTPGTTAALLYQGA
                     GPQVVQPSAKSTVAPNSPQTTTAPSAPLPMSMEDRQAQPAPMASRQYPALPAGAQAKP
                     VLKRPAAVVNKQAVARVQAQQAAMKAQTSQQSGLAPRDMQSLGEGLGSAVRTFRDAAA
                     DIKNSIDPQMRTIQAELESAEKEMQDSIEVAKEPMNPKERSGSA"
     gene            2329..2649
                     /locus_tag="PKM72_14555"
     CDS             2329..2649
                     /locus_tag="PKM72_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62066.1"
                     /translation="MKKNQITKLEGILQHSSWLEVVLGSVPVEDQESYREKSHDLLVQ
                     LLCFHKLHAFIHQGIKQENIQVSGDALVAETDKVVELEAEEIKRQIFIELDLVETEDG
                     PHSL"
BASE COUNT          718 a          576 c          736 g          761 t
ORIGIN      
        1 ttattttaaa accgagtttt ggatttttcg ttattgagaa gaaagaggga tagccgtata
       61 gcctttagac tccaggcacc cttctaagga ataaatatcc ttttcccttt gagtgcttcc
      121 tgttgagagc aaagcttcct cgcaatccga tttatcacgg gtaaaggttt cctgtgaggt
      181 gccagacctg taccacttca ccgggtctga ggcacaactc acgctcgaaa gtattgtcat
      241 taagcctatg agcagattta tttttatttt cttcatgtat tgcctcgttg agaaaaagaa
      301 ggcgaattgt aataacttta tctcgagtat tcaatcaggc gcattgaata tggaaagatt
      361 ctaatctagg gacagtattg actgggaatc tgagagaatc atgaataggt cgaccaggaa
      421 aaattggtgg agattaggta ttcggaccaa tttggacaag agcccaggat gaccaaaaat
      481 gcacattggg ggtcgggtct gtttccatgg cttgtttcag tgcaaacaca tccgcactgg
      541 ttcccaattt gcctaaacga aatgcggctt ccgatcgcac tcccacgctt gggtcttgga
      601 gaagatgggt gcgtaagtat gggagtcctc tgggatcacc taattcaccg agccctgcaa
      661 ttgccgattg acgtgtgaga gaatctggac tttccagagc ttggagatac acggaatagg
      721 aaaatggaaa ttcaaagtga gcgagagatt ggataatggt tctttgggtg tcaattgtgg
      781 aaatagcgta ggcttcggtt aatacctgca ataaatcctc atgcactgct gttcgaccca
      841 aggccaatac cacagctttt ttgactgaat cggatggatc tgtaaggtgc tgaaccaatg
      901 caactagaga ctgatccgat aaggattcgg ccattgttcc caaggcccat gcacttgatt
      961 gtcgaacctg gtcatccggg tccgataagg cgagaacaag ggctggtacg ctctgaggat
     1021 caccaatttt ccctagtgat aaagctgcgg tccgtcgaat atcaggctga gtatcggtca
     1081 gaagtggaat aagtcgttcg gtgacagttt cgggtggttc aggtggggat tcaataacac
     1141 atccaatgag tccatagaaa caaatggctc cgataaggat agcaggactg gtagggaatg
     1201 gtagcaagaa aagattaggc agatcctgaa cgttctttgg ggttcatagg ctctttagcc
     1261 acttcgatgg agtcttgcat ctctttttcg gcagactcca attcggcctg aatggtgcgc
     1321 atttgaggat cgatcgaatt tttaatatct gctgcggcat cccggaaggt gcgtacggca
     1381 cttccaagac cttctcctaa tgattgcata tctcgtggag ctaatccgga ctgctgggaa
     1441 gtctgggctt tcattgcagc ttgctgggct tggactctgg ccacggcctg tttattgaca
     1501 acagcggcag ggcgcttcag cacgggcttt gcctgtgccc cagccgggag ggcggggtat
     1561 tgcctcgaag ccataggggc gggttgcgct tgcctatcct ccatggacat cggcaacggt
     1621 gcagaggggg ctgtcgtggt ttgaggagag ttaggggcta cagtagattt agctgaaggc
     1681 tggaccactt gaggccctgc tccttggtaa agtaacgctg ccgtggttcc aggcgtttgt
     1741 tcaggaccag gctggaatgg ttgattggtc tgggtgccta ccggggtagt cggttgggat
     1801 tgagcctgtg ctggttcggg cggtgcagat ggaagtggct cattggggtc cacaggttga
     1861 gggatatcgg cgacttcctt cttaaagcct tttagagctt tcccgacacc ttcccctatt
     1921 tgcgggagac gacctgcccc gaaaataatc aggatgatca ccagaatgat catgagttca
     1981 gagaacccca tggttccgaa catagagaag cccctttccc ttcagcttac tgtgaaaata
     2041 ttcaactaaa aagactctat ctttcaagat tgacgagtgt caacctaaat acggtaggct
     2101 tagaaggcta tgcccaagcc aagtttttgc gtattcttta tactgtgtta tgccctttaa
     2161 ttagtgagtg ggtccagaat gtcattactc tcaccacctc agattcccct ctttcggatt
     2221 accctttttt acggccccga agccgttgaa ggtgactcag attcagtgca ctgtgttttt
     2281 aatgttaaaa agcgcagctg gaaaggcggg gtgcaagttg aggtggtaat gaaaaagaac
     2341 caaataacca agcttgaagg aatacttcag cattcatcct ggttggaagt cgttctaggg
     2401 agcgttccgg tggaggacca ggagagctat cgcgaaaaaa gtcatgattt gttggtacag
     2461 ttgttgtgtt tccacaagct ccatgccttt attcatcagg gaatcaagca ggagaatatc
     2521 caggtttccg gagatgctct tgttgctgag acagataagg ttgttgagct ggaggcagag
     2581 gaaatcaaga ggcagatttt cattgagttg gatctggttg aaacagaaga tggcccccat
     2641 tcactatgaa tgtattaaat ttgctataat tttttgtttt ttatttaaaa acagaagact
     2701 gacgaatcat tcatgattag aataacaacc agaaggtttg cataaaaatt aaacaggaag
     2761 gagtattcct tatgcggcaa tccaagggtt t
//
LOCUS       DAORAQ010000306         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5030_length_2779_cov_78.189060, whole genome shotgun sequence.
ACCESSION   DAORAQ010000306 DAORAQ010000000
VERSION     DAORAQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5030_length_2779_cov_78.189060"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PKM72_14560"
     CDS             <1..174
                     /locus_tag="PKM72_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNP62067.1"
                     /translation="YEKELRRAREIAFQEIQEEALRVGANAIVGIDLDYEVMGETGSM
                     LMVSISGTAVRVE"
     gene            398..871
                     /gene="bfr"
                     /locus_tag="PKM72_14565"
     CDS             398..871
                     /gene="bfr"
                     /locus_tag="PKM72_14565"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNP62068.1"
                     /translation="MKAKKGVVDFLNKVLTNELTAINQYFLHGEMCGNWGYEQLHNEI
                     RKHSIDEMKHAEEIIEHLLYLEGVPNMQRLGTIKIGETVPEQFKSDLALEHEAVVLLA
                     EAIAHCTTVGDFTTRAKLEGIIKSEEEHIDWIETQLETIKQVGVENYLSQHMHEE"
     gene            complement(1031..>2779)
                     /locus_tag="PKM72_14570"
     CDS             complement(1031..>2779)
                     /locus_tag="PKM72_14570"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNP62069.1"
                     /translation="ALHVDGMPINMRSHAHGQGYGDINFIIPETIEEITVKKGPYHVE
                     YGDFATAGAANYVTRETVPQTVVQSAGGNFNTQRHLFMTSPTHDRFRTLLAGEFYYTD
                     GPYDFVNRNTRYNGLAKLTFDPSATSQLSVTLSQYYGRWNGSGQLPLRDVRSGGVDRF
                     GSIDPSEGGKSLRSTGRLDYHYDLPGGGTVFANLWAQYYYLSLFSNFTFYLNDPVNGD
                     GIEQTDRRWLTGSDIGYRQIFRLLDYEGTMTAGLQTRFDQIQVRLGTQQKRSSLAITQ
                     ESDIFEASYSPYLKLDLQFLPWMRFVGGGRVDVFTYNVKDRCGADCSERPNGTASNAI
                     ASGKANLVFGPWYQTEFFLNVGTGFHSNDARDAVENPSTNSLTRAIGYEIGIRSRPWA
                     WSEFLATFWLLDLGSELVFVGDEGTTEPRGKTRRLGTEFSSRITPLDWLTIRGDVTYT
                     HAEFRKTGDAVPLAPQFTAFSSVTARFPIGLSGTLQMLTVGSRAGTEDDNVKLEPFTI
                     FDLVLRYKIPLAPPTGRLEAFFSIRNLTDTDWRQAQFYYESRLPGEPAGGVADIHFVP
                     GTPRMFMGGLTWFFPT"
BASE COUNT          756 a          631 c          722 g          670 t
ORIGIN      
        1 tacgaaaagg aattgcgacg cgcgcgtgaa attgcctttc aggaaattca ggaagaagcc
       61 ttgagggtag gagccaatgc cattgttgga atcgatttgg attacgaggt tatgggggaa
      121 accgggagta tgctcatggt gagtataagc gggactgccg tgagggttga gtgaccgaat
      181 gggggacgga gggagagctt cacaccttca taaactcgaa aggttgaact tgaatattca
      241 gatcaacgaa gttgagaaag gaattcaatg ttaaataatt ttccctaaat aaaaacattg
      301 accaaaatga agttgggccg ataaactatg tcagtcgaat gaaacagaaa ttctcatttc
      361 ttacgtctga aacaatcttt aataaagggg gacttccatg aaggcaaaaa aaggcgtcgt
      421 cgattttctg aataaagttc ttaccaatga actcaccgcg atcaatcaat atttcctaca
      481 cggcgaaatg tgtggaaatt gggggtacga acaattacat aacgaaatta gaaaacactc
      541 aattgatgag atgaagcatg cggaagagat tattgagcat cttctttatt tagagggtgt
      601 tccgaatatg cagcggttgg gaacgattaa aattggggaa acggttcctg agcaatttaa
      661 atcagatttg gctcttgagc acgaagcggt tgttcttctc gccgaggcca ttgcccattg
      721 taccactgtg ggagatttca ctacacgggc aaagctcgaa ggtatcatta aaagtgaaga
      781 agagcatatc gactggatag aaacccaatt ggaaacaatt aagcaggtgg gagttgaaaa
      841 ttatctcagc caacatatgc atgaagagta gccgtttctt tttctcaaac ttgacagggc
      901 tttccgggat tgccgatgca tcctggaaag cccttttttc atggaatgat tgagggtgag
      961 ctcgatcgtg aaacagtctg catggaaggc cggttataga gtctggtgaa tgcgatcatg
     1021 gtcctgcccg ttatgtcgga aaaaaccacg tcagcccacc catgaacatt cgtggagtgc
     1081 cggggacaaa gtgaatatcg gcaacacctc cggcgggttc cccgggaaga cgggactcat
     1141 aataaaactg ggcctgacgc caatcggtat cggtcaaatt gcggatgcta aaaaatgcct
     1201 ccaggcgtcc ggttggtggc gcaagaggga ttttgtagcg aagaaccaga tcgaaaatcg
     1261 taaatggttc cagtttcaca ttgtcatctt ccgtgccggc acggcttccc acggtcaaca
     1321 tttgcaacgt tcccgatagt ccaatcggaa agcgggctgt gacggatgaa aacgccgtga
     1381 actgcggcgc caatgggacg gcatcccccg tttttcggaa ctcggcatgc gtatacgtaa
     1441 catcgcctcg aatggtgagc caatccagag gggtgattcg agagctgaat tctgttccca
     1501 ggcgacgcgt ctttcctcgc ggttccgttg tcccttcatc ccccacgaat accaattcag
     1561 atcccagatc cagcagccaa aatgtggcga gaaattcgga ccaggcccac gggcggctcc
     1621 tgataccgat ttcatagcca atagctctgg ttaagctgtt agtcgatgga ttttccacgg
     1681 catcgcgcgc atcattgctg tgaaaaccgg ttccaacatt caagaagaat tccgtctggt
     1741 accagggacc aaagacgaga ttggcttttc cactggcgat ggcattagag gctgtcccgt
     1801 ttggtctttc tgagcaatct gccccgcaac gatctttcac attatacgtg aagacatcaa
     1861 cacgaccgcc tcccacaaat cgcatccagg gaaggaattg aaggtccaat ttcagatagg
     1921 gagaatagga ggcctcgaat atgtctgatt cctgcgtgat cgccagggac gaacgtttct
     1981 gttgcgtgcc caaacgcacc tggatttgat caaagcgtgt ctgaagtcct gccgtcatgg
     2041 tgccttcata atcgagcagg cgaaaaatct gccgataacc gatatcactt ccggtcaacc
     2101 atcgtcggtc ggtctgttca atcccgtcac catttactgg atcattaaga taaaacgtga
     2161 agttgctgaa cagggacagg taataatatt gagcccacag attggcgaat actgtccctc
     2221 cgccgggtag atcgtaatgg taatccaatc gaccggtgct ccgtaacgat ttgccgcctt
     2281 ctgaaggatc aatggaacca aagcggtcca cgcccccgga cctcacgtcc cgcaagggaa
     2341 gctggcctga cccgttccat cgtccataat attgagaaag cgtcacactg agttgagatg
     2401 tggctgaggg atcaaacgtc agtttcgcca gcccgttgta tcgggtattg cgattgacaa
     2461 aatcataggg gccgtcggtg taataaaatt ctcccgcaag aagggtgcgg aatctatcgt
     2521 gagtgggaga agtcatgaac agatggcgtt gggtattaaa attccctccg gcggattgga
     2581 ccacggtttg gggaactgtc tcacgggtga cataattggc agcgccggcg gtggcaaaat
     2641 caccatattc cacatggtag ggtccttttt ttaccgtaat ttcttcgatg gtttccggaa
     2701 taatgaaatt gatgtccccg tatccttgac catgagcatg gctgcgcatg ttgatcggca
     2761 tgccatccac atgaagggc
//
LOCUS       DAORAQ010000307         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5031_length_2779_cov_67.196769, whole genome shotgun sequence.
ACCESSION   DAORAQ010000307 DAORAQ010000000
VERSION     DAORAQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5031_length_2779_cov_67.196769"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKM72_14575"
     CDS             <1..1041
                     /locus_tag="PKM72_14575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNP62070.1"
                     /translation="LFSTKPGQPIPYYHYNRQLSVVMARTFTDLGFAVDVISNQNKGF
                     LPKKKYDFFIDTRMNFERLAPHLNKDCIKILHATTAHPHFNNFAETKRLLALQERRNV
                     TLRSRRFMEPSFAMAIEYADCVVVHCAFGVKNFNYANKPIYLVPNAVPYRYPWSDSKD
                     FESCRFRFLWLGSEGMVHKGLDLVLEAFAGMPEYHLTVCGPVAREKDFEQAYFQELYH
                     TPNIHTRGWIDVESAEFTDLTNRCLGLVYPSCSETNAGSVLTSLHAGLIPIISYESGV
                     DVGKDEGVVLEDCSIEAIREAVRQTSSRSTQELRRMSRKSWEFAQENHMVNNFEKNYR
                     DVIEKLISENKK"
     gene            1146..1898
                     /locus_tag="PKM72_14580"
     CDS             1146..1898
                     /locus_tag="PKM72_14580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP62071.1"
                     /translation="MVLKLKQLIKKIPGAYSAYQWIVKICKEVASRLKSTEAIFTEIY
                     KENYWRGENSVSGQGSDLEQTQTIVKELPILFDKLKISTMLDIPCGDFFWMKRVDLRG
                     IHYTGADIVGELVERNRYLYQTHNIIFTKLNLLRNDLPQVDLIFCRDCLGHFSFSDIF
                     MALQNMSRSGSTYLLTTTFPERQANRDIKTGQWRTLNLEAVPFQFPPPQSLLVEHCTQ
                     KTSRGEYKDKSLGLWKISDIEIISKRLPSSYL"
     gene            2061..>2779
                     /locus_tag="PKM72_14585"
     CDS             2061..>2779
                     /locus_tag="PKM72_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62072.1"
                     /translation="MKKFTISPLVLVGVIIISLVIGALIWRLSSPYFGVVRGFLSPQL
                     ENVGSGPWSIGIYKGESPFELHDPEDISNPVLTAKDVKDVDAKFVADPFMTTKDGRFY
                     LFFEVLNRANNQGDIGYAESEDGKKWEYKNILINEPFHLSYPYVFEWESNDYLILESH
                     EDLSIRLYRAKSFPAEWQFVKSIFNGEHYVDPSIFRYENIWWLFYTNPQNDALNLYYS
                     DELMGDWRPHPMNPIVKLNKH"
BASE COUNT          818 a          508 c          623 g          830 t
ORIGIN      
        1 cttttttcaa ccaaaccggg acaaccaatc ccttattatc actataaccg tcaattgtcc
       61 gtcgtgatgg caagaacctt tactgatctc gggtttgctg tggatgtgat tagtaatcag
      121 aataagggtt ttcttcctaa aaagaaatat gattttttta tcgatacccg gatgaacttt
      181 gaaagactcg cccctcacct taataaagac tgtattaaga tacttcatgc gactaccgcc
      241 catccccatt ttaacaattt cgccgaaacg aaacgacttt tggctttaca ggaaagaaga
      301 aatgtaaccc tacgctccag gagattcatg gagccaagct ttgccatggc aattgagtat
      361 gcagattgtg tggtcgttca ttgtgcattt ggtgtcaaaa atttcaatta tgctaataag
      421 ccaatttatc tagttccgaa tgcggttccc tacaggtatc cctggtcgga ttcaaaggat
      481 ttcgaatcct gtcgattccg atttttgtgg ttggggagtg agggaatggt gcataaaggt
      541 cttgatttgg ttttagaggc attcgcagga atgccggaat atcatttgac ggtttgcggt
      601 ccggtcgcga gagaaaagga ttttgagcag gcatattttc aggagttgta ccatactcct
      661 aatatacata ctcgtggatg gattgatgtt gaaagcgctg agtttaccga cctgaccaat
      721 cgctgtctcg gattggtcta tccatcatgc tctgaaacga atgccggttc ggtgttgaca
      781 tcgctgcatg ctggccttat cccaatcata agctatgagt ccggtgtgga tgtgggtaag
      841 gacgaaggtg ttgtccttga ggattgctca atcgaggcga ttcgggaggc ggttcgtcaa
      901 acttcgtctc gttctactca ggaactcaga cgtatgtcca ggaaatcatg ggagtttgct
      961 caagaaaatc atatggtgaa taatttcgaa aaaaattatc gagacgtgat agaaaaattg
     1021 attagtgaga ataaaaaata atattcaaga tttagttgtg gttattagtc tgagtttaaa
     1081 cgcgaaatag ccaattgcta aaagaccgtc tagagaatta aaaaaggatt ttaacgatat
     1141 tagagatggt attaaaatta aaacaattga taaaaaaaat tccgggtgcc tattctgcct
     1201 accaatggat tgtgaaaatt tgcaaagaag tggcttcacg tctgaaatcc acggaggcta
     1261 tattcactga aatatacaag gaaaattatt ggaggggtga aaatagtgta tccgggcaag
     1321 gttcagatct tgagcaaaca cagacgattg tgaaagaact gccaattctc tttgataaat
     1381 tgaaaatttc cacaatgctt gatatccctt gtggcgattt tttctggatg aaaagggtgg
     1441 atttaagagg aattcattat acaggagcag atatagttgg agaattagtt gagcggaatc
     1501 gatacttata tcagacacat aatatcattt ttacgaagct aaatctttta cgaaatgact
     1561 tacctcaagt tgatcttatt ttttgtcggg attgtttagg gcatttttcc ttttcggata
     1621 tttttatggc cttgcagaac atgagtagga gtggatcgac ctatttgctc acaacaacat
     1681 ttcctgaacg acaagccaac cgcgacataa aaaccggcca atggaggacc ctgaatcttg
     1741 aagcggtccc tttccaattt cctccccccc aatcacttct tgtagaacat tgcactcaaa
     1801 agactagtag gggggagtac aaggataaat ctttggggct ctggaaaatt agtgatattg
     1861 aaatcatttc aaaacgactg ccctcctctt atttatgagg gagaattttg tggccacaga
     1921 tttcctgacg aatgttggtg gcagttttag gtttttactc tgagtggaaa aaatgaaatt
     1981 ttttatgctc ctatagacgg acagggaaga tcgctggacg ctttctctct aggcactgac
     2041 taattggaag ttagccatta atgaagaaat ttacaatatc cccacttgtc cttgttggag
     2101 taatcatcat tagcctggta ataggagcat tgatctggag gttgagttct ccgtactttg
     2161 gggttgtacg aggcttttta agtccgcagt tagagaacgt tggttccggt ccctggtcga
     2221 ttggaattta caagggtgaa tctccatttg aattacatga tcctgaagac atctcgaatc
     2281 ccgttcttac ggcaaaggat gttaaagatg tcgatgccaa atttgtagcc gatcccttta
     2341 tgacaaccaa agatggtcgt ttttatctgt ttttcgaagt gttgaatcgg gccaataatc
     2401 agggagatat tggatatgcc gaaagcgagg atggtaaaaa atgggaatac aagaatattc
     2461 ttatcaatga accgttccac ctatcctatc cttacgtgtt tgaatgggaa agcaatgatt
     2521 acttaatact ggaaagccac gaggatctct cgatcagact atatcgggcc aagtcatttc
     2581 ctgcggaatg gcaattcgtg aaaagcatat tcaatggaga acactatgtg gacccctcaa
     2641 tttttcgata tgaaaatatc tggtggttgt tttatacaaa cccgcaaaat gacgcattaa
     2701 acctctacta ttctgacgag ttaatggggg attggaggcc tcatccgatg aatccgatcg
     2761 tgaaattaaa taagcatat
//
LOCUS       DAORAQ010000308         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5194_length_2722_cov_65.594676, whole genome shotgun sequence.
ACCESSION   DAORAQ010000308 DAORAQ010000000
VERSION     DAORAQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5194_length_2722_cov_65.594676"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PKM72_14590"
     CDS             complement(<1..631)
                     /locus_tag="PKM72_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62073.1"
                     /translation="MANMTDSSGDPFKEFESPVALGEWLAEQDKEGFATLWEKWRSDV
                     TGVPIELATMDRKLVFTYTGMGVVKPPLKIDVKGDLLEQTYALLTAVQQSVSRLPRET
                     DTGTLVLPTTEPGLIGLPPAKSERSGLPAESLPSPPGFEPPSIASPLLDPGIPSVIVL
                     PLPAVDGPVRPGLPEPAAVTAVISASTVSTVRLLPDEAPSAVVPLPVSAG"
     gene            complement(708..1193)
                     /locus_tag="PKM72_14595"
     CDS             complement(708..1193)
                     /locus_tag="PKM72_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62074.1"
                     /translation="MALKFKYALVEINSKPVLVDKKAFAEASQALRDALAKVSAITKE
                     VKEKGFALQTPEGMIPVNLGSASDFSKWVTNKLEIKNFDLGKEVAELPSPARDVANIL
                     INTDLVLYSAALFYTGEPLKKLYGELVIGFKLPENAMDFPLALKEIVIAVDNYPKDPK
                     G"
     gene            complement(1292..1516)
                     /locus_tag="PKM72_14600"
     CDS             complement(1292..1516)
                     /locus_tag="PKM72_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62075.1"
                     /translation="MGAMSTHTSNTETNEKPTNKKQKKEIEDDIIGKLTHYSELMRES
                     NSIKTSMNFHLDAKQINSDDKVEIQMKFLA"
     gene            complement(1597..1839)
                     /locus_tag="PKM72_14605"
     CDS             complement(1597..1839)
                     /locus_tag="PKM72_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62076.1"
                     /translation="MQEQQDELINVVQERTSSLLVTNACLKQEIAEHRQAEHKLREQH
                     AQLRALASEVLEAEERERRYMPGGLAMQGLTLNTFL"
BASE COUNT          616 a          725 c          686 g          695 t
ORIGIN      
        1 ggccggccga cacaggcagg ggcacgacgg cggatggggc ctcgtcgggc aacaggcgca
       61 cagtcgagac cgtggacgcc gatatgactg cagtcacggc agccggctcg ggcaggccgg
      121 gccgaaccgg tccatcgacg gcgggcagtg ggagaactat caccgacgga attccggggt
      181 ccaacagagg gctggcgatt gacggcggct caaacccagg aggagaaggg agggattccg
      241 ccggcaggcc ggatctctcg gacttcgccg gcggcaaccc gatgagaccg ggctcagtcg
      301 tcggcaaaac gagtgtaccg gtgtctgttt cccggggaag acggctaacg gattgctgaa
      361 ccgccgtcag cagtgcgtag gtctgctcca gaagatcccc ctttacgtcg atcttgagcg
      421 gcggcttaac gacccccata cccgtataag tgaagacaag cttgcgatcc atcgtcgcga
      481 gttcgatggg cactcccgtt acatcactcc gccacttttc ccagagggtc gcgaagcctt
      541 ctttgtcctg ctctgcgagc cactccccca gcgcgacggg actctcgaac tctttgaaag
      601 gatctccaga tgagtcagtc atgtttgcca aagcgatgac ctcggttgga ggatttgtct
      661 ggagccatta tcgccttgtt cctctacgaa cttttttatt ttttagttta accttttgga
      721 tcttttggat aattgtccac cgcgatcacg atctccttca atgcgagtgg gaaatccata
      781 gcattttccg gtagtttgaa cccaatcacc agctcaccgt agagtttctt caagggttcg
      841 ccagtataga acagcgccgc agaatagagc acaaggtcgg tattaattaa aatatttgcc
      901 acgtccctag ctgggctggg gagttctgct acctcttttc caagatcgaa attctttatt
      961 tctaatttat tggttaccca tttagaaaaa tcactcgccg accccagatt gacaggtatc
     1021 atcccttccg gggtctgcaa ggcgaatcct ttctccttca cttctttggt gatggccgat
     1081 acctttgcca atgcgtccct tagggcctgg gaggcctcag caaacgcttt tttgtccacc
     1141 aatacgggtt tgctgttgat ctcgaccaag gcgtatttaa atttaagtgc cataggagta
     1201 ttcctttctt tcccagccat tcaaccgagt ttattatggg tgacgtattg tgtgctatgt
     1261 gaactcgaac actttgcggt ggagggagag ttcatgcaag gaatttcatt tgaatctcga
     1321 ccttgtcgtc gctattgatt tgttttgcat ccagatggaa gttcatgctc gtcttgatgc
     1381 tgttgctctc ccgcattaac tcggagtagt gcgtcagctt gccgatgata tcgtcttcga
     1441 tctccttctt ctgcttcttg ttcgtcggtt tctcattcgt ttccgtgttt gaagtatgag
     1501 ttgacatagc tcccattgtt tctctctgcg gaaggcgaat ggtctgttga gcatccaatt
     1561 tcggtcaagt tgcaaattcc cgctttcttg tggaatctaa aggaacgtgt taagggtcag
     1621 gccttgcatt gcaagaccac cgggcatgta ccgccgttcc cgctcttcgg cctctaagac
     1681 ttctgacgcc aacgcccgga gttgggcgtg ctgttcacgc agcttatgct cggcctgcct
     1741 atgctcggcg atctcctgtt tcaggcaggc attggtcacc agcagggagg aggtgcgctc
     1801 ctgcacaaca ttaatcagtt cgtcttgctg ctcttgcaaa gcccctttgc accgtctgag
     1861 atcagtgaca tcaacgcacg accctaagta tccgccaggg cccccctctt atgtgcgcga
     1921 tggcacactc atatgcaaga cccaacaata ttggccatcc ttccggcgct gacggtcatc
     1981 gggatgaacc gcttccgttc acgtcgtcct cagcgtttcc tccagagttc gccccgtaaa
     2041 catcagccat gacttattga aatacgtgcc gcgcgtatcc attccagcaa tccagatcat
     2101 gccaggcatc gtatcggcca tgatgcggaa tttctcttgc tcgcttggca gtgtgttgtt
     2161 gacgagcttc tcgtctcatt tcttttgtag gaaaatgcaa gattcgccct ctttttagca
     2221 gaatgaagta attatagaaa ccccaaaggg ataaactttg cctctgaaac cacagcaaca
     2281 aatctggaag gcggtagcgc tatggctgca atcacaacat ctggttagat caaagccggt
     2341 ggccgcccac aaagccataa aactttcagg tagttgtcag atgataagta tgcgacaccc
     2401 aggtgcccac ggcgaatgct gcctggggca cggcagtcag ggggaacacc agtgacacaa
     2461 agaatgtcta cactgacaac cggcggggat aacagaagaa gaaggttgcc cataaaggca
     2521 cgaggcttgc ttcaattact gtacatgcag agctcctgat ttcatgattc ttacgagaaa
     2581 gataccatcc acatggttca accttttaaa gacaaaggac aacacatgca ccccgataac
     2641 gcggaaatgt taaaagtgaa aatttgagga aaaaccatcc tgtctgttga gccatccaga
     2701 tggtccggtg aggtcaattc tc
//
LOCUS       DAORAQ010000309         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5198_length_2720_cov_71.866792, whole genome shotgun sequence.
ACCESSION   DAORAQ010000309 DAORAQ010000000
VERSION     DAORAQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5198_length_2720_cov_71.866792"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(618..1394)
                     /locus_tag="PKM72_14610"
     CDS             complement(618..1394)
                     /locus_tag="PKM72_14610"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNP62077.1"
                     /translation="MSKIYDALQIAYGEKLGSGNESKKEPIEVPSVSIPSPSLLQVRN
                     TTSCPRLYNESELMVMAQNIAALLPSPDKNVIQFIGSRKGEGTSTLVREFALVSSQHS
                     NKPVLLVEADLMHPSQYQAFGIEAKSPLDHVLKDGKALDGVVSQVDQSNLFLATLSSK
                     VQSALTTRTFYNPTDMWKIVREQFSLILIDSSPANVTADSLALCETVDGIVLVVEAEQ
                     TRSAVAKKVRDQILMQEGNLLGMVFTKRHLYIPESLYKFI"
     gene            complement(1404..>2720)
                     /locus_tag="PKM72_14615"
     CDS             complement(1404..>2720)
                     /locus_tag="PKM72_14615"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP62078.1"
                     /translation="TPDPNFLYLSPSHKEAMGAIIYGIERRKGFVAIYGEVGVGKTTI
                     IRAYLEKTSDRKQKTVYILNPVLSFHGLLTDIFRSLDLVPSHDDSAEMVNQLQEALIQ
                     EYRRDSTVVLVIDEAQNMPVKTLEQLRLLSNLETSTDKLIQIVLIGQPELTTLLNHPT
                     LKSLNQRIALRATIHPLQQKESQAYIEHRIALSSSTGAPLFTQGAMKLIIREAQGIPR
                     RINILCDNALITGYGKQQKPVSINEVREILVDMNGSRSSYLVKWAVGVAVVILLAIGA
                     FLLSPIFLPGNAGVSQVGLSERDSQNVRATSSEKMSQESIGQPGEPLMPMASSGSEID
                     RPIPHTDRGGDGVMTKTPVEKVIESAKSDGKIRDHTEAASKSSSVLPVTRAVREGDNL
                     SQIANEAYGSSSRQYVEWLRRHNPEISDPDIILPGQKIVLPEYVKE"
BASE COUNT          748 a          624 c          623 g          725 t
ORIGIN      
        1 gcttggcatc gcggtcccgc tcccacgccc atgggaccaa ctatgcaccg gagcatgaat
       61 ttcgtgcaag atactctagc ggggagacgg tcgtttcgga ttctccctgt cttggataac
      121 tgaaggtgcc acagaccagc gctagaggcg gggtttcaaa tgtcgggaaa attgcgggcc
      181 gggccttgga ttgtgcgctg aaagggaaac caggaccgcg gtcgataact gtggatcatg
      241 acagcgaatt cctatcacgg gcagttgcga gacgagtgct caagcattca tcaggtcgag
      301 tcgctcaatg ccgttcaaac gatcatcgaa acgtgggaac aggaatccat gccgcctaca
      361 tagatcgctc ggacatctga caccgaatga gtttatgaga caacgtcatg tcaatcaaac
      421 agccgaaatc gccctctttt ctggttgaga actacctctg aatgggaccg acggcacaaa
      481 acagaaaaat ctagttttca tcaaactgta tttggggaaa ccttactacc gcagagctcc
      541 tcagatgaaa caataaaaaa agtaatgtga agttaaacga cagaggaaaa acaggcagtt
      601 tggaaggaaa gggaaaccta aatgaattta tagagagatt ccgggatgta taaatgccgt
      661 ttggtaaaca ccataccaag gagatttcct tcctgcataa gaatctgatc tctgactttt
      721 tttgcgacag ccgaacgggt ttgttcggct tcaaccacca acacaatacc atcaacagtt
      781 tcacaaagtg ccaagctatc tgctgtgaca ttggccggag acgaatctat gagtatgagg
      841 gaaaactgtt cacgaacaat tttccacata tcagtaggat tgtaaaaagt tcgtgttgta
      901 agagctgatt gaactttgga agataatgta gctagaaaaa gattcgattg gtcaacctga
      961 gaaacgaccc catcaagggc tttcccatcc tttaatacat gatccaaagg ggatttggcc
     1021 tcgatcccga atgcctgata ctgcgagggg tgcataagat ctgcttcaac aagaaggaca
     1081 ggcttattgc tgtgctgaga ggaaaccaga gcaaattccc gaacaagcgt ggacgtacct
     1141 tcgccctttc ttgacccgat gaactgaatg acatttttgt cagggcttgg aagaagtgcg
     1201 gcaatgtttt gtgccatgac catcaattcg gattcattat aaagccttgg acaagacgtt
     1261 gtatttctaa cttgtaataa agaaggcgac gggatcgata cagaaggaac ttctatgggt
     1321 tcttttttgg actcattccc ggaacccaac ttttccccat aggcaatctg caaagcatcg
     1381 taaattttac tcataatgtt gtcttattct ttcacgtact caggtaacac aattttttga
     1441 cctggaagaa tgatatcggg gtcgctgatt tctgggttat gccgacggag ccattccaca
     1501 tattgccggc ttgaagaacc ataggcttca ttggctattt gactcaaatt atccccctct
     1561 ctgacggccc tggtaactgg taaaactgag cttgactttg aagccgcttc agtgtgatcc
     1621 ctgattttac catcagactt ggctgattct attacctttt cgactggggt tttcgtcata
     1681 acaccatccc ctcctctatc tgtatgagga ataggtctat caatttctga gccagatgag
     1741 gccataggca tcaacggctc tccaggttga cctatgcttt cctgagacat tttttcagag
     1801 gaggtcgccc tcacgttctg actgtcacgt tcgctcaggc ctacttggga tactccagcg
     1861 tttccaggaa gaaaaatcgg gcttaataaa aaagcaccaa ttgctaagag tatgaccaca
     1921 gcaacaccga ccgcccactt cacaagatat gaggaacgag agccattcat gtcaacaaga
     1981 atttctcgca cctcgttaat agagaccggt ttttgttgct tgccatatcc agtaatcagc
     2041 gcattatcac aaagaatatt aattctccgt ggaatcccct gagcctcacg aataatcagt
     2101 ttcatggctc cttgcgtaaa taaaggcgcc ccggtggatg atgatagtgc aatccgatgc
     2161 tcaatatagg cttggctttc tttctgttgc agcggatgga ttgttgcccg gagggcaatg
     2221 cgctggttta aggatttcag ggtaggatga ttcagcaatg ttgttaattc tggttgcccg
     2281 attaacacaa tttggataag tttgtctgtg gatgtttcca gattagaaag cagccgcaac
     2341 tgctccaatg ttttaaccgg catattttga gcttcgtcaa ttaccagcac aacggtgcta
     2401 tcccgccggt attcttgaat caacgcttcc tgaagttgat tgaccatctc agcggaatca
     2461 tcatgagacg ggacaaggtc caggcttcga aatatgtcgg tcagaagccc atgaaaagat
     2521 aatactggat taagaatgta gactgttttc tgtttccggt cactcgtctt ttcaagatac
     2581 gcacgaataa tcgtcgtttt ccctaccccg acttcgccat aaatggcgac aaaacctttt
     2641 cttctctcaa ttccatagat aatagccccc atcgcttcct tatggctggg gctcagataa
     2701 aggaaatttg ggtcaggagt
//
LOCUS       DAORAQ010000310         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5324_length_2672_cov_66.557126, whole genome shotgun sequence.
ACCESSION   DAORAQ010000310 DAORAQ010000000
VERSION     DAORAQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5324_length_2672_cov_66.557126"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKM72_14620"
     CDS             complement(<1..760)
                     /locus_tag="PKM72_14620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNP62079.1"
                     /translation="MTHAFDLGDALKQLGQPNETTTGKPKEGTPEKKTGKKAPSLTDL
                     TDLVKGTSTEEEIAIGQEIAGRLLGAAPLVEDERLQRYVNQVGKWLSLQSGRTDLDWY
                     FGVIDSDDINAFAAPGGFIFLTKGLYTQLHSEAELAGVLGHEIGHVIKQHQLTIIKQS
                     QAIEMGSNLFSRKLGKMKNEQAKLAVNNAIGSGAEVMARGLDKDAEYQADRIGVVLAT
                     RAGYDAYGLPMVLQEIGHAGLNDSSVALLFKTHPH"
     gene            complement(917..1432)
                     /locus_tag="PKM72_14625"
     CDS             complement(917..1432)
                     /locus_tag="PKM72_14625"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNP62080.1"
                     /translation="MKNWVAFLTIVLIWVFPAGGNAADPGIAVRSCQVMSEPFKDAQE
                     VTSLKEGDTVEILKRKGGWLQVSQKGHTGWVRMLYIRRGASAENVSAATEASGLLGLA
                     TGRAGSGNVVAATGVRGLNEEELKEAKFNEHELQKLKTYRASKKQAQEFANQAGLTVQ
                     KVPFIQPTDGN"
     gene            complement(1515..>2672)
                     /locus_tag="PKM72_14630"
     CDS             complement(1515..>2672)
                     /locus_tag="PKM72_14630"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNP62081.1"
                     /translation="IALVLPAFTGILESGRMGTNNVYPDRDGIVRQYPIYRDHYGWRV
                     PSLPAKLGETLGWRLPDDSDVYLNWRGKFGAFRSVRFSEVFDDFLRRDGLRAPDEFTN
                     KIVIIGSSASALFDTKPTPMEKIHPGVEILATAIGNIKNGDWITQPKNPWLFTALALG
                     LIWVTALAFLTGVKRKAIDMVFAGSQVGLMAFTFASLNLTTYYLDLTAPITAGLIYFS
                     LARVYAYAEATIMEKYVWINVEEGVDGWQYTVVVVLQPESLEKISEARFLTLLKRGLY
                     GKKVGFTIEAFSRKPAGIEKAFQNMFLIYWVENHVETKPWEVGKSGEQTVSLVREVAR
                     SICENDRIGCKLGWCQGPLTYGDEKSRLEAWQQLVVKAMVNLKDQPLVITS"
BASE COUNT          642 a          752 c          582 g          696 t
ORIGIN      
        1 gatgagggtg agtcttaaac agtaaggcga cgctgctgtc attgagaccc gcatgaccaa
       61 tttcctgtaa caccatgggc aggccataag cgtcatagcc ggccctggtg gccaacacca
      121 ccccaatcct gtcggcctga tattcggcat ctttgtccaa cccacgagcc atcacctccg
      181 ctccactccc gatagcatta ttcacagcca gcttggcttg ttcatttttc attttgccaa
      241 gttttcggga aaacaggttg cttcccattt caatcgcctg gctttgttta ataatggtca
      301 gctgatgctg tttgataaca tgaccgatct catgccccag gactccagcc aattccgctt
      361 cagaatgaag ttgtgtatac aaacctttcg tcagaaaaat aaatcccccc ggcgcagcaa
      421 aggcgttgat gtcatcggaa tcaatcaccc caaaatacca atccaggtcc gttcttccac
      481 tctgtaaact gagccatttc ccaacttgat tgacataccg ctgcaggcgt tcatcctcga
      541 ccaggggggc cgctccaagg agtcgtccgg caatctcttg ccctatggct atttcctctt
      601 cggtggaagt ccctttgacc agatcggtca agtctgtcag cgaaggagcc ttcttgccgg
      661 tttttttctc cggagtacct tcctttggct ttccggtggt ggtttcattc ggctgcccca
      721 attgtttgag ggcatcaccc agatcaaagg catgagtcat ggttgccacc ccggagaaca
      781 cacatgtcag aatgaaagca cgcagaagga atcctttcgg gccaggaatt gcctgttgct
      841 tgtggaatat cagagaaaca gaccgtaacg attcagaggg tagacagttc ttcatagtat
      901 gctgattgct cattttttaa ttcccgtctg tcggctgaat gaacggaacc ttttgcacgg
      961 ttaaccctgc ctgattagca aattcttgtg cctgtttctt tgaagcccga taggttttca
     1021 gtttctgcag ttcatgttca ttaaacttag cctcttttaa ttcctcttca tttaatccac
     1081 gcactcccgt agcggccaca acatttccac tcccagctct cccggtagcc aggcctaaaa
     1141 gtcctgaagc ttccgtcgcg gccgaaacat tttccgcgga ggccccccga cgaatatata
     1201 acatcctgac ccatccggta tgacctttct gggacacctg taaccagcca cctttccttt
     1261 tcagaatttc tacggtgtcg ccttctttta atgatgtcac ttcttgagca tctttaaagg
     1321 gttcgctcat gacttggcag gatcggacgg ctatccccgg atccgcagcg tttccccctg
     1381 caggaaagac ccaaataagg acaattgtca ggaatgctac ccagtttttc atcaacgtcc
     1441 tccttcttgc atggaaccga ccaggcggtt cccggtgaac cactgtctga ggcatggggc
     1501 ttcaggataa cggattatga tgtaatcaca agcggctggt ctttaagatt caccatggct
     1561 ttcaccacta attgttgcca agcctccagc ctcgatttct catctccata cgttagaggt
     1621 ccttggcacc atccgagctt acatcctatt ctatcgtttt cacagatgct gcgagcaact
     1681 tctcgaacca gagataccgt ctgttcgccg gatttcccca cctcccatgg ctttgtttcc
     1741 acatgatttt ccacccaata aatgagaaac atattttgaa aggctttttc aatgccggca
     1801 ggtttccgcg aaaaggcttc tatcgtaaac ccgacttttt tgccatagag accccgcttt
     1861 aacagggtca ggaaccttgc ttccgaaatt ttttccaaac tctccggttg aaggaccacc
     1921 accacggtat attgccaacc gtccacaccc tcttccacat taatccagac atatttttcc
     1981 atgattgtgg cttcggcata ggcatacacc cgcgcgagag aaaaatagat caaccctgcc
     2041 gtgatgggtg cggttaaatc aagatagtag gtggtgagat tcaagctcgc gaaggtaaag
     2101 gccatcaaac ccacttgcga accggcaaac accatatcaa tggctttacg tttgaccccg
     2161 gttaaaaatg ccaacgccgt gacccagatg aggccaagcg ccagagcggt aaagagccag
     2221 ggattcttcg gttgggtgat ccaatctcca ttcttgatat tcccgatcgc ggtagccaga
     2281 atttccacgc cgggatgaat cttctccata ggcgtgggtt tggtatcgaa cagtgcagag
     2341 gcgctggacc cgataatcac aattttattc gtgaattcat ccggcgcccg cagcccgtct
     2401 ctacgcaaga aatcatcaaa cacttcgctg aagcggacag accggaaggc cccaaatttc
     2461 ccacgccagt tcaaatacac atctgaatca tccggtagtc tccatcccag ggtttccccc
     2521 agctttgctg gaagggaagg cacacgccac ccatagtgat cccgatagat agggtactgt
     2581 cgaacaatgc catcccgatc aggataaaca ttgttggtgc ccattcttcc actctcaaga
     2641 atgccggtga aggcgggcag aaccagggca at
//
LOCUS       DAORAQ010000311         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5369_length_2653_cov_68.156274, whole genome shotgun sequence.
ACCESSION   DAORAQ010000311 DAORAQ010000000
VERSION     DAORAQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5369_length_2653_cov_68.156274"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..672)
                     /locus_tag="PKM72_14635"
     CDS             complement(193..672)
                     /locus_tag="PKM72_14635"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNP62082.1"
                     /translation="MGVSGSGKTTIGQLLARDLGWSFYEGDNFHSQKNVAKMREGMPL
                     NDEDRLPWLQALHNLVLDLITKAQRAVITCSALKQTYREWFTEGQPQVALVYLRGNYT
                     LIHRRLQSRTMHFMKADLLASQFRALEEPHDAPFVDVSQAPEVIVEQIKRMLPLHPA"
     gene            complement(724..1263)
                     /locus_tag="PKM72_14640"
     CDS             complement(724..1263)
                     /locus_tag="PKM72_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNP62083.1"
                     /translation="MNDQPFLTDVKTLRKRAREHIEKGAVTQGYAADRDTVIKLLNEA
                     LATEIVCVLRYKRHYYMASGIHAQSVAAEFLQHANEEQGHADQLAERIVQLGGEPNLS
                     PDGMLMRSHSEYVEGTSLVDMIKEDLVAERVAIDSYREVINYLSTNDSTSRRMLEGIL
                     AVEEEHADDLVSLLEEMGS"
     gene            1674..>2653
                     /locus_tag="PKM72_14645"
     CDS             1674..>2653
                     /locus_tag="PKM72_14645"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HNP62084.1"
                     /translation="MKAVVYNGPQDVSVKEVPDAKIERPTDVLVKMTTTNICGSDLHM
                     YEGRTAVESGTVFGHENLGEVIEVGKGVTQVQVGERVCLPFNISCGFCKNCERGLTAF
                     CLTVNPGTAGGAYGYAGMGPYPGGQAEYLRVPYGDFNCLRLPEDAQEKEADYVMLSDI
                     FPTGYHATEMAGVQPGDSVVIYGAGPVGLLAAYSAILKSASKVMIVDRHPDRLRLAEK
                     IGAIPIDDSKVCPVDQVLELTGGEGADTGCECVGYHAHTPQGHEQANMTMNNLVGSVR
                     ATGGIGVVGVFMPEDPKSFDALAQKGQLTFDWGLFWSKGQRIGTGQANVKA"
BASE COUNT          635 a          648 c          688 g          682 t
ORIGIN      
        1 ttaatctatc cgcatgggag caatgaaacc atctggtata agtggaaaaa ggaaaaagac
       61 caatccccca gaatgtccta atgctctgat agatgtgata gggaaatgac ctcgctcaag
      121 gtcaaaatct ctgcaaacgg gcgcaacata cctcttattt tcagccgttc ttatcatgac
      181 gtcgcagaga tgtcatgctg gatgtaaggg cagcattctt ttaatttgct ccacaatcac
      241 ttcaggtgct tgagaaacat caacgaatgg agcgtcgtga ggctcttcga gcgctctgaa
      301 ctgactggct aacaggtcgg ctttcataaa gtgcattgtt cgggattgca ggcgtcgatg
      361 gataagtgtg taattgcctc gcaaatacac taatgccacc tggggttggc cctcggtaaa
      421 ccactcgcgg taagtctgct ttaaagcgga acacgtaata acggcgcgct gggccttcgt
      481 gatcaggtcg agaacaaggt tatgcaaagc ctgaagccag ggaagccgat cttcgtcatt
      541 caaaggcatc ccttcacgca ttttggcaac gttcttttgt gaatggaaat tatcaccttc
      601 gtaaaatgac catccgagat ctctggccag gagttgacca atggtcgttt ttcctgaacc
      661 agagacaccc atcaggatta caatccatat gtcccgttcc tttgtctctc tcatagccaa
      721 actttaggaa cccatttcct ccagaagcga gacgaggtca tcagcatgct cttcctcgac
      781 agccaaaatg ccctccagca ttcggcggga tgtcgaatcg tttgtgctca gataattgat
      841 cacttcccga tagctatcaa tggcaacacg ctctgccacc aggtcctcct tgatcatgtc
      901 gaccagggat gttccctcca catattcgga atgactacgc atcagcatgc catccggaga
      961 gagattcggt tcgcctccca gttgtactat ccgctccgcc aattggtcgg catgaccctg
     1021 ctcttcattg gcatgttgaa gaaactccgc cgccacactc tgagcatgaa ttcccgatgc
     1081 catgtaatag tgccgcttat atctgaggac acagacgatt tcagttgcca gggcttcatt
     1141 gagtagctta atgaccgtgt ctcggtcggc cgcataaccc tgggtcaccg ctcctttttc
     1201 gatgtgttct cttgcgcgtt tgcgtaaggt tttcacgtct gtgagaaatg gttggtcatt
     1261 cattcccgcc tcctaacgtt gttgattctt cttcatgagc agccatgcca gtccataaac
     1321 cttccttagc cttttcccat aacaggcgta tatagacggc cttcatttga ataatcagaa
     1381 ttttctcagt cctgtcgtca gtggacaatg cacaaaattt ccaattcgtt ctggatttta
     1441 tccaaaatac caaagaaacg tggaaaacgt aggcggagag ttacttattt gggtcagcca
     1501 gggttcttct cagtgccatc ttcagcctgt ttcctctcta atactatgta tattgactcc
     1561 gtcgagcttc agatgtggtt tgccgataat gtcgtccacc attcactgtg gcagcggctg
     1621 tgcctctcac acaaattgga atatccctca caaacagagg aaggaattat gccatgaaag
     1681 cagtcgtgta taacggtccc caagatgtca gtgtgaaaga agttcccgac gcaaaaatcg
     1741 agcgaccgac ggatgtgctc gtgaaaatga cgacgaccaa tatttgcggg tctgatttac
     1801 acatgtacga gggacggacg gcggtcgagt caggcacggt gttcggacac gagaaccttg
     1861 gcgaggtgat cgaggtcggc aagggggtca cacaggtcca ggttggcgag cgtgtgtgtc
     1921 tgcccttcaa catcagttgc ggattttgca aaaactgcga gagaggtttg actgcgttct
     1981 gtctgacggt caatccggga accgctggtg gagcttatgg ttatgcggga atgggtccgt
     2041 atccgggtgg acaagcggag tacttgcgtg ttccttacgg tgatttcaat tgtttgcgtt
     2101 tgcctgagga tgcacaggaa aaggaagccg actatgtcat gctttcggac attttcccta
     2161 ccggatacca tgcgacggag atggctggtg ttcagccggg cgactcggtt gtcatttatg
     2221 gcgccggacc cgttgggctg ctggcggcct attcggcgat cctcaaaagc gccagtaagg
     2281 tcatgattgt ggatcgtcac ccagatcgat tacgactggc cgagaaaatc ggtgctattc
     2341 ccatcgacga ctcgaaggtt tgtccggttg atcaggtcct tgagctaacc ggcggagagg
     2401 gagcggatac gggatgtgag tgcgtgggat atcatgctca tacccctcag ggtcatgagc
     2461 aggccaatat gactatgaat aatcttgtag gatcagtgcg agcgaccggc ggcattggtg
     2521 tggtgggcgt gtttatgcct gaagacccca agtcattcga tgcattggct caaaagggac
     2581 agttgacatt cgactggggg ctcttctggt ctaagggaca gcggatagga accgggcaag
     2641 ccaacgtgaa ggc
//
LOCUS       DAORAQ010000312         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5397_length_2645_cov_65.437452, whole genome shotgun sequence.
ACCESSION   DAORAQ010000312 DAORAQ010000000
VERSION     DAORAQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5397_length_2645_cov_65.437452"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKM72_14650"
     CDS             complement(<1..773)
                     /locus_tag="PKM72_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNP62085.1"
                     /translation="MPQKCGQLDASTFVDPSNKVIHRGGRVHPAFTIDLFIYDISIVN
                     PLTRIRNYRFMLQDFWQEFLIVWHIGILNTSLGDIFLALSIFVMFLFARRIVFRFLSH
                     VFKKLATRTQTDTDARILDAIERPLEFTFVIIGLYISGQVVSLSPPLNAVFGQIIRSL
                     IAFTIFWSIFRILDPLSILLDRFITFFGNQTMHETIKGFFLKVSKFIVVCLGVAAVFQ
                     EWGFNVAAVLGSLGLVGMAVALGAQDFIKNMFAGLTIFM"
     gene            995..1381
                     /locus_tag="PKM72_14655"
     CDS             995..1381
                     /locus_tag="PKM72_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62086.1"
                     /translation="MAYTYPSWLGRIFVLMGLVGCGGTQEPGDLRQADIGFGIVEGTK
                     GFMQIQEEALSPYGSLRVVEGQVFQIEGAAYVVHINDQIEARLPFDENTRIDRPAHVG
                     DWIEAQLDQEGRARIIRNIDDRISLE"
     gene            complement(1560..>2645)
                     /locus_tag="PKM72_14660"
     CDS             complement(1560..>2645)
                     /locus_tag="PKM72_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62087.1"
                     /translation="GRCIVVFAYDVGASIQIDEAEQRITATKERGRIKRKRPAPEYFD
                     YHPLPLRITQELEPISLGAHYTLHTVDTVLYDFGALMVTYQVPLAGPFASLLPLSEVL
                     YENAQLLEGTFLHIQSLLNTLGPAIEKPNISELVEDYAIFHIFSTQPALDLSKFVKQH
                     GTLLSQILRCEDQPLSEQEICDATTSQIAFGTQDLTLIDWNASLMIGQEMDDVRAVLE
                     FANVELVERRFLDHQLDEALDEAYAALTQETRHGFRWPGSSDVNLRRIAGMQVDSAIL
                     FERVTNTLKLFGDQYLARVSRLASQRFHLHSWDTSILRKLDTLDNLYDKMAGQTGNRR
                     MEILEWIIIILIAISILLPFIPGFPGY"
BASE COUNT          681 a          574 c          741 g          649 t
ORIGIN      
        1 tccatgaaaa ttgtgagtcc ggcaaacata ttcttaatga aatcctgtgc cccaagggcg
       61 accgccatgc cgaccagtcc taagctacca aggaccgccg cgacattgaa tccccattct
      121 tgaaatacgg cggccacgcc taagcagacc acgatgaatt ttgaaacttt gaggaaaaag
      181 cctttgatgg tttcatgcat ggtttggttg ccgaagaacg tgatgaaccg atctagcagg
      241 atggaaagcg gatcaagtat tcgaaaaata gaccagaaaa ttgtgaaggc aatcaaagat
      301 ctgatgattt gtccaaagac cgcatttaac gggggagaca aggacacgac ctgtcctgaa
      361 atatagagtc cgataatcac aaaggtaaat tcaagggggc gctcaatggc gtccaggatt
      421 cgggcatccg tatccgtttg agttcgtgtc gcgagctttt tgaagacatg tgacagaaag
      481 cgaaacacaa ttcgtctggc aaacaggaac atcacaaaga tgcttaaggc taagaaaatg
      541 tcacccaagc tggtattcaa aatgccgatg tgccatacga taagaaattc ttgccaaaaa
      601 tcttggagca taaatcgata gttcctgatt cgagttaaag ggttgacgat ggagatgtcg
      661 tagataaaga ggtctatcgt aaaggcagga tgaacgcgtc cacccctgtg aatgaccttg
      721 ttggacggat cgacgaatgt cgatgcatca agttgtccac atttctgcgg caagggtaaa
      781 agaattttcc ggtaaggtca atttttatgc tggaattgga aagatgtgtt ggtgccacca
      841 gtgagggatt gggccagagg aagcaacgtg tctggcacaa gatggtgtga aatgaatcca
      901 cgttcatgga cagcctgaat gagtgtgtta ggatgcattc tcggagtgat gttcatcagt
      961 catctgaggg gcaagcaagg agggagggct gtccatggca tacacgtatc caagttggtt
     1021 gggcaggatt tttgttctca tgggtttggt gggttgcggg ggaacacagg aacctgggga
     1081 cttgcgtcag gcggatattg gatttggcat tgttgaaggg accaaaggtt tcatgcagat
     1141 tcaagaagaa gccttgtctc cctatggatc gttgcgagta gtggaggggc aggtgtttca
     1201 gattgaaggg gcagcgtatg tcgtgcatat caacgatcag attgaggccc gattgccttt
     1261 tgatgaaaat acgcgaattg atcgaccggc tcatgtcggt gattggattg aggcgcagct
     1321 tgatcaggag gggcgtgcca ggatcattcg aaatatcgat gaccgtattt cactcgaata
     1381 agctccttcc tcttcacatt ttcccttttg cggtttctcc ttgtggcgtc ccaatagtcc
     1441 gctctcagca tgctgttgtt gcaccttttc tcatctcaga acgttgagtg gtgtcgttgc
     1501 ccccatgggg gttgttggag ggatcaaggg cggggaacag ggagaaaggg ggaggccgtc
     1561 taatagccgg gaaatcccgg aataaacgga aggagtatgg agatggcaat gagaatgatg
     1621 atgatccatt caagaatttc catccgccga ttgccggttt gaccggccat cttgtcatac
     1681 aaattatcca gcgtgtccaa cttgcggaga atgctggtat cccaactgtg caaatgaaag
     1741 cgttgcgagg ccagtcgaga gactctggcc aggtattgat cgccgaataa tttcagcgtg
     1801 ttggtgaccc gttcaaagag aatcgcgcta tccacctgca tgcctgcgat tcggcggaga
     1861 ttaacatccg atgatcccgg ccaacggaac ccatgccggg tttcctgtgt gagggcggca
     1921 taggcctcat ccaacgcttc atccaattgg tggtcaagaa atcggcgctc gacgagttcc
     1981 acattggcga attctagaac cgcccggacg tcatccattt cctgtccaat catgagcgag
     2041 gcattccaat caatgagggt caggtcctgt gtcccgaagg caatttgact cgtggtggca
     2101 tcacaaattt cctgctctga caatggttga tcctcacaac ggaggatttg agacagaagc
     2161 gtgccgtgtt gtttgacaaa tttcgacaga tccaacgcag gttgcgtcga gaaaatatga
     2221 aatatcgcat agtcctcgac caattcggat atattcggtt tttcaattgc cggacccagc
     2281 gtgttcagga gagattggat atgcaggaat gtgccttcga ggagttgagc attttcatag
     2341 aggacttcac tgagcgggag caacgacgca aacggcccgg ccaggggaac ctggtaggtc
     2401 accatgagcg cgccgaagtc atagagtacg gtgtcgacgg tgtgaagcgt gtaatgtgct
     2461 ccgagactga tgggttccag ctcttgcgta atacggaggg gcagggggtg ataatcaaaa
     2521 tattccggtg ccggccgttt ccgtttgatt cgtcctcgtt cttttgtcgc ggtaatccgc
     2581 tgttccgctt cgtcgatttg aatggacgca cctacgtcat aggcaaagac gacgatgcat
     2641 ctccc
//
LOCUS       DAORAQ010000313         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5438_length_2630_cov_116.942524, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000313 DAORAQ010000000
VERSION     DAORAQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5438_length_2630_cov_116.942524"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..82)
                     /locus_tag="PKM72_14665"
     tRNA            complement(7..82)
                     /locus_tag="PKM72_14665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Lys,seq:ctt)
     gene            complement(230..305)
                     /locus_tag="PKM72_14670"
     tRNA            complement(230..305)
                     /locus_tag="PKM72_14670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(270..272),aa:Arg,seq:ccg)
     gene            complement(455..>1341)
                     /locus_tag="PKM72_14675"
     CDS             complement(455..>1341)
                     /locus_tag="PKM72_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62088.1"
                     /translation="GLAILVSLISVNMPSQAWSAEDTTSSRPNPRHPWPALLQQADTL
                     GLPTGFLKHIDPTFVTVHFEDLRTYAAEYHPHDHTMILNLRLSFNEAGGALADLARMT
                     HHDVALLYHELLHAYLDYLYATDHGQALTAEDQQVLEAANNQMACHYRFVRINPIRQL
                     KGATELRFLSDQDTWEVMNETWAVFVGWVLWTKLELFQDHLTTKNWTPALIDQWTQRL
                     TDAVQSGELLGYYEPDDPEERQIARKRFIAPSNGLTPQDVELLLTTVLGESPELVQAS
                     TAVFEQYQGSMEPAPSCE"
     assembly_gap    1342..1441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1442..>2630)
                     /locus_tag="PKM72_14680"
     CDS             complement(<1442..>2630)
                     /locus_tag="PKM72_14680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNP62089.1"
                     /translation="VRNLAATHDNVMVVGDDSQSIYAFRGATFRNIMEFPDLFPGAVI
                     YKLEENYRSTQPILRLANKLIEAAPEKYSKTLFTRKEEGPLPTVVEAMGENAQSRFIA
                     QKILELREEGIPLHEIAVLFRSSFHAFDLELELTRRNLPFIKRGGFKFIETAHVKDLM
                     AHLRIIHNPLDTVSWNRLLLLLEGVGPKKAKDLIASILQAHGQYEVLKHSTGRSASGL
                     RQLAATLDQVTEDPLTPAALLGEMMEYYVPLLKDQYDDYPKRIRDLEHLSVMAERYES
                     LNDFLADLTLEPPNESVVDVDAPDRDDERLILSTIHSAKGLEWQCVFVIWLVDGRFPS
                     AYSFMGPEELEEERRLLYVAVTRAKHHLFLTYPINVYDKMTGSVLTKPSRYLDHIPPS
                     YFDT"
BASE COUNT          545 a          616 c          749 g          620 t
ORIGIN      
        1 taaatatggt gggccgtgta ggggtcgaac ctacggcccg ctgattaaga gtcagctgct
       61 ctaccaactg agctaacggc cccaccgaga tgcagaatat tttagaaaag tattttacgg
      121 tttttccaga acctcgtctg gatatcggag aattctccct cccctatccc ctccttacaa
      181 atgaaaggga tcatttttat ctctttattc gctcaaaaat tcatgtgcat ggtgcgcctg
      241 acaggatttg aacctgtggc cccttgctcc ggaggcaagt gctctatcca ctgagctaca
      301 ggcgcaagat tgtgttcatg tgcaattaca tccggcctct agttcttgtg gcctctccgc
      361 catgcgagag gaaaaacaga gggcaaccgc cccggaaaag ttctgaagct agcatggatg
      421 tttaggtgat gtctacctgc cggaggccat gaatttattc gcaggagggc gccggttcca
      481 tgctgccttg gtactgctca aagaccgcgg tggaggcctg gaccagttcg ggtgattccc
      541 ctaagaccgt ggttaacaat agttcgacat cctgtggcgt aaggccattg gacggggcaa
      601 tgaagcgttt acgcgcgatc tggcgttcct ccggatcatc gggttcataa tagcccaata
      661 attctcctga ttgcaccgca tctgttaagc gttgggtcca ttggtcgatg agtgcgggtg
      721 tccagttttt tgtggtcagg tgatcctgga agagttccaa ttttgtccag agcacccagc
      781 cgacaaagac ggcccaggtt tcgttcatga cttcccacgt atcctgatcc gagagaaatc
      841 gcagttcggt tgcccctttg agctggcgaa taggattgat gcggacaaat cggtaatggc
      901 aggccatctg gttgttggcg gcctccaata cctgctggtc ttccgcggtc agagcctgcc
      961 catggtcagt ggcgtagaga tagtccagat aggcatgcag gagttcatga tagagcaggg
     1021 caacatcatg gtgagtcatc cgggccagat cggccaacgc gcccccggct tcattaaagg
     1081 acagacgcag gttgagaatc atggtgtgat cgtgtggatg atattctgcg gcataggtgc
     1141 gaaggtcttc gaagtgtacc gtgacaaatg tggggtcgat gtgtttgagg aaccccgttg
     1201 gcagccccag ggtatcggct tgctgcagaa gggcaggcca cggatgccgg gggttgggac
     1261 gacttgaggt ggtgtcctca gcggaccagg cttggcttgg catgttcaca cttatcaggc
     1321 tgaccagaat ggccaggccc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 ngtgtcgaaa taggatggcg ggatgtgatc cagataccgt gagggtttcg tgaggaccga
     1501 ccctgtcatt ttatcgtaca cattgatggg gtaggtgagg aagaggtggt gtttggctct
     1561 ggtgaccgcg acgtagagca gacggcgctc ttcttccagt tcttcagggc ccataaagga
     1621 gtaggcggag ggaaaccgtc catccaccag ccagatgaca aagacgcatt gccactctaa
     1681 gccttttgcc gaatgaatgg tggaaagaat cagccgctca tcgtcccgat cgggggcatc
     1741 cacgtccacc acactttcat tcgggggctc gagtgtgaga tcggccagga agtcgttgag
     1801 actttcgtaa cgttccgcca tgacggataa atgttccagg tcccgaatcc gcttggggta
     1861 atcgtcatac tggtctttga ggagaggaac atagtattcc atcatttccc cgagcagcgc
     1921 ggcgggggtg aggggatctt ccgtgacctg atccagtgtg gcggccagtt ggcgcaatcc
     1981 cgaggcggag cggccggtgc tgtgcttcaa gacctcatac tggccatggg cttggaggat
     2041 ggacgcgatc aggtctttgg cttttttggg gccgacccct tcgaggagca agaggagccg
     2101 gttccaactg acggtgtcca acggattgtg aatgatccgc aaatgggcca tgaggtcttt
     2161 gacatgggcc gtttcgatga atttgaaccc gcctcgtttt atgaatggga ggtttcggcg
     2221 ggtgagttcc aattcgaggt caaaggcatg aaagctggat cggaacagca ctgcgatctc
     2281 atgtaagggg ataccttctt cccttaattc cagaattttt tgagcgatga atcgcgattg
     2341 ggcattttcc cccatcgcct ctacgacggt gggtaatggt ccttcctcct tccgcgtaaa
     2401 cagcgtttta ctgtattttt ccggcgctgc ttcgatgagt ttgttggcca gtcggagaat
     2461 cggttgcgtg ctccggtagt tttcctccaa tttgtaaatg acggccccag ggaagaggtc
     2521 gggaaattcc ataatattgc ggaaggtggc gccgcgaaag gcgtagatag attgcgaatc
     2581 gtcgcccacc accatgacat tgtcatgggt ggccgccaga ttgcggacca
//
LOCUS       DAORAQ010000314         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5444_length_2629_cov_62.537296, whole genome shotgun sequence.
ACCESSION   DAORAQ010000314 DAORAQ010000000
VERSION     DAORAQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5444_length_2629_cov_62.537296"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /gene="groL"
                     /locus_tag="PKM72_14685"
     CDS             <1..1159
                     /gene="groL"
                     /locus_tag="PKM72_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055846.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNP62090.1"
                     /translation="MDKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAD
                     RMEASLEDAFLLIVEKKISAMKDLLPILEQVAKMGKPLVIVAEDVEGEALATLVVNKL
                     RGTLNVAAVKAPGFGDRRKAMLEDLATLTGGQVISEEVGLKLESVKLTDLGRAKRVNI
                     DKDNTTIVEGAGDPKRIEGRVKQIKTQVEETTSDYDREKLQERLAKMVGGVAVINVGA
                     ATETEMKEKKARVEDALHATKAAVEEGIVPGGGVALLRCVPALDKMKLNHDQQVGVNL
                     VKRSLEEPIRQISINAGAEGSIIVEKVRSETTNRGYNAGTGEFVDMVEAGVIDPTKVA
                     RCALQNAASVAGLMLTTEVMVTEIPEAKPEGAGMPGGHDHGMGGMGGMGGF"
     gene            1489..1863
                     /locus_tag="PKM72_14690"
     CDS             1489..1863
                     /locus_tag="PKM72_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62091.1"
                     /translation="MVFLITGNGLLGIINKGEAADSRSRTEANCTIGMMKGETKAECR
                     VPIPNGCAVAQFPEYPEPWVDISQGGGTSCQFNEKQTDWKTSIVGSCQACTTEQCTGR
                     FMVMFNCVNNVPPANPGTPGQK"
     gene            1897..>2629
                     /gene="queG"
                     /locus_tag="PKM72_14695"
     CDS             1897..>2629
                     /gene="queG"
                     /locus_tag="PKM72_14695"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNP62092.1"
                     /translation="MTPRSILANQIKEEARRLGFDAVGIARIPTPLPSTPCPPAQSTI
                     PLAEEPITLRLWGRLKKWLDLRFHGTMEWIAKDPKRRSNPAEVLPGCQSLVMVGMNYY
                     TDHTPDESKLSGRIARYAWGKDYHGFMKDRLTQLESFLHSHVPDIKTRSYVDTGPIME
                     KAWAQEAGIGWIGKHTNLVSTDFGSWLLLGEILTTLSLDGDEPAVDLCGTCSLCIQAC
                     PTGAIVEPYLLDAERCISYLTIEYRG"
BASE COUNT          711 a          582 c          700 g          636 t
ORIGIN      
        1 aatggataaa gtgggaaaag atggcgtcat caccgtagaa gaagccaaat ccatgactac
       61 gtcattagat gtagttgaag gaatgcagtt tgaccgcggg tacatctctc cctatttcgt
      121 gaccaatgca gaccggatgg aagcttcctt ggaagatgcg tttttgctaa tcgttgaaaa
      181 gaaaatcagt gccatgaaag acttgctccc tattcttgag caggtagcca aaatgggcaa
      241 accattggtc attgtggctg aagatgtcga aggcgaagct ctggcaacgt tggtggtcaa
      301 taagcttcgt ggaacattga atgtggcagc ggtgaaggct cctggctttg gtgatagacg
      361 gaaagccatg ctggaggatc ttgccaccct gacaggcgga caggtgattt ctgaggaagt
      421 aggcctcaag cttgaaagtg tgaagctgac agaccttgga cgcgccaaac gagtgaacat
      481 tgacaaggat aataccacga ttgtggaagg tgccggggat cccaagcgga ttgaaggtcg
      541 agtcaagcaa attaagacgc aggttgagga aaccacatcc gattacgatc gggaaaaatt
      601 acaagagcga ttagcgaaaa tggttggtgg tgtggcagtc attaacgtag gcgccgcgac
      661 cgaaaccgaa atgaaggaga agaaagctcg tgtagaggat gcccttcatg cgaccaaggc
      721 tgcagtagag gaaggcattg ttcctggtgg aggcgtggct cttcttcgtt gcgttcctgc
      781 tttggataag atgaaattaa atcacgacca acaagtcgga gtgaacctcg tcaagcgttc
      841 cctggaagag cctattcgac aaatctccat caatgcaggt gctgagggat ccattattgt
      901 ggaaaaagtt cgaagcgaaa ccacaaaccg aggatacaat gccggcaccg gagaatttgt
      961 ggatatggtt gaggctggtg taattgatcc aaccaaggtc gcacgttgtg cgttgcaaaa
     1021 cgctgctagt gtggcaggcc tcatgctcac cacggaagtt atggtgacag aaatcccaga
     1081 agcgaagcca gaaggggctg ggatgcctgg cggccatgac cacggcatgg gcggcatggg
     1141 cggcatgggc ggattctaaa attctgctta cagatatagt aaaagccccc tggaggattg
     1201 aatcttccag ggggcttttc tactcctgaa cctttattcc ttccccgttc agacctcatc
     1261 atacaatttt gcaataccca gaattagtct gcataaggga tagatttttg acaggttgtt
     1321 tgggtgatgt ggtataagga aatccatctt tgcagaactc cccagtcgta ttcgtgcaga
     1381 tttccacacc cttttacccc gctacaggtc gctatgaatt cgtttttaag cacaatcaaa
     1441 ataagatttt cccaacaaaa aaccagtcat ctattgatgt gggcaattat ggtgtttctc
     1501 attaccggga acggcttgtt ggggattatc aataaggggg aagcagctga ttcccggtca
     1561 cggacggaag cgaattgtac cattggcatg atgaaaggcg agacaaaggc tgaatgccgt
     1621 gttccaattc ccaatggctg tgctgttgcc cagtttcccg agtatcccga accctgggtt
     1681 gatatttccc aaggaggggg gacttcttgt caatttaacg agaagcaaac cgactggaaa
     1741 acgtcgattg tcggatcctg tcaagcttgc acaactgagc aatgtacagg aagatttatg
     1801 gtgatgttta attgcgtaaa caatgttcct cctgctaatc cgggaacccc tggacagaaa
     1861 taaatctcgt tttttgcttg agaaggagga gtactcatga ctcctcggtc aattctggcg
     1921 aatcaaatca aagaagaggc ccgccgctta ggttttgatg cggtaggaat tgctcgcata
     1981 ccaacgcctc ttccaagcac gccctgccct ccggctcaaa gtacgattcc ccttgcggaa
     2041 gaaccgatta cattgcggtt atggggacgc ctaaaaaaat ggcttgactt gcgatttcat
     2101 ggaactatgg aatggatagc caaggacccg aaacgccgtt ccaacccggc agaagtgctt
     2161 ccaggttgcc aatcactggt aatggtcggg atgaattatt acaccgatca tactccagat
     2221 gagtcaaagc tttctggacg cattgcccga tatgcctggg gaaaagacta tcacgggttc
     2281 atgaaggacc gattgacaca attagaaagc ttccttcact cacacgtacc ggatattaag
     2341 acgaggagct atgtggatac gggtcccatc atggaaaaag catgggctca agaggccggc
     2401 atcggctgga ttggcaaaca taccaatctg gtttccaccg attttgggtc ctggctgtta
     2461 ctcggagaaa ttctaacgac cctgtcattg gatggtgatg aacccgccgt ggatttatgt
     2521 ggaacctgct cgctctgtat ccaggcttgc cccactggag ccattgtgga accttatctc
     2581 cttgatgcgg aacgatgcat ctcgtatttg acgattgaat accgtggta
//
LOCUS       DAORAQ010000315         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5459_length_2624_cov_61.571429, whole genome shotgun sequence.
ACCESSION   DAORAQ010000315 DAORAQ010000000
VERSION     DAORAQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5459_length_2624_cov_61.571429"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKM72_14700"
     CDS             <1..339
                     /locus_tag="PKM72_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB family protein"
                     /protein_id="HNP62093.1"
                     /translation="NLLFWILANGIPSGIGGLLAMAHPLTIVAAFFSAPFTSLTPVIG
                     VGYVTAFVQAYLQPPLVREFQTVAEDIAIPRKWWKSRLLRVFLAFLLPTIGSLIGTWV
                     GGTRIVSNLF"
     gene            606..1352
                     /locus_tag="PKM72_14705"
     CDS             606..1352
                     /locus_tag="PKM72_14705"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNP62094.1"
                     /translation="MNATKILKGMVLGVVLLLPASGWSETNDEDLPRLTVSAEGTINV
                     PPDKAVLSFAVETVGEKLSDVQQGNQERVAKVLEECRRFNIPSEFIQTTSLNVIPEYP
                     PPPRRQADGTLENNVPRIIGYRVVHQVNVEVRNLEIVGKVVDRVLHVGANKFSGIAWG
                     LQNEQPTKLEVLKQASAKAQAKAEALAQALNLKLVRMINVSEGGNSPSPPEGRYRMAM
                     AMDSGGDASVSAGEISIRGSVLLVYEISQK"
     assembly_gap    1439..1538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1539..2440
                     /locus_tag="PKM72_14710"
     CDS             <1539..2440
                     /locus_tag="PKM72_14710"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNP62095.1"
                     /translation="ETEKAGTDLGVPALVVSGFLGAGKTTLVKHLIADAQRQGLKLAV
                     ISNEFGELGIDRALLQEEGGPGYVELEGGCVCCQLSGELLNTLQNLWETIHPDRVVVE
                     TSGVALPFETLLTFWREPVTEWVGESLAVVVVNAEQVAQGRDLEGTFEQQVSSADLLV
                     INKVDLVPSDSFGRLEDLLHDMAPGAPVIRSIQGEIDTTIVFPSIPAAKTSSNAQRKP
                     ELLPHTHEEFEAEELSFPDNITPEQLLKHLQPLNTLRIKGIVNTSEGPTLVQGVGPRI
                     DLSAPPSQFPQEMLGRLVVIRRK"
BASE COUNT          598 a          597 c          675 g          654 t
ORIGIN      
        1 aatttattgt tttggatttt ggccaatggt attcccagcg ggattggcgg gttgttagct
       61 atggctcatc cgctcaccat tgtcgccgca tttttctcgg caccgtttac cagcctcacc
      121 ccggttattg gagtcgggta tgtgacggcg tttgtccagg cttacttgca accgccattg
      181 gtgcgggagt ttcaaaccgt agcagaggat atcgccattc cacgcaaatg gtggaagagt
      241 cgactcctgc gtgtgttctt agcgtttctc ctgcccacga ttggaagtct catcggcacc
      301 tgggtgggag gcacgcgtat tgtgtctaat ttgttttgat caggagcaaa ttaaagccaa
      361 gcacagagag gctaggactg gggcgcggcg actttagacg tgtgctggaa aatacatttc
      421 gggcaggtat agtgaataaa gcgaccatcg ccaacgagac gtttcagcat agacgtttga
      481 caccaggtgc agagacggtc cggcagatct gatgtagaag gtgtcaaggg aggcatggaa
      541 tccgtgttag tcataatagg ggtcattctc aaaaacatac agggggtttg tcaatgttta
      601 gggctatgaa tgccacaaaa atattgaagg gtatggtgct gggggttgtg ctgttgcttc
      661 cggcatccgg gtggtccgaa acgaatgatg aggatcttcc acgattaact gtttccgcag
      721 agggaacgat caatgtgcct cccgataaag cggtattaag ttttgcggtt gaaacagtcg
      781 gggaaaagtt gtccgatgtt caacaaggaa accaggagcg tgtggcgaag gtcttggagg
      841 aatgccgacg gtttaatatc ccctctgagt tcattcaaac cacgtccctg aatgtgattc
      901 ctgaatatcc gccacctccg cggcgtcaag cggatggaac cttggagaac aatgttcccc
      961 ggatcattgg ctatcgtgtt gtccaccagg tcaatgtgga agtacgtaac ctggagattg
     1021 tggggaaggt cgttgaccga gttttacacg ttggggccaa caaattttca ggcatcgcct
     1081 ggggcttgca aaacgaacaa cccacaaaac ttgaagtctt gaaacaggct tctgccaaag
     1141 cccaagccaa ggctgaagcg ttggctcagg ctttgaatct caagttggtt cgtatgatca
     1201 atgtttcgga aggtggaaat tccccatccc ctcctgaggg tcgatatcgg atggcgatgg
     1261 ccatggatag tggcggagat gcctcggtgt cggcaggaga aatctccata cgtggttccg
     1321 tcctcttggt gtatgaaatc agccagaagt aatcaatcct tttttttatc agtcttgtat
     1381 cccccgtcct tgtcctcatg agcattccct tcatgaacaa tgaaaagcca gaaactgann
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gaaactgaaa aagccggaac
     1561 ggatcttggg gttccggcac tggtggtgtc cggcttcctg ggggcgggta agacgactct
     1621 tgtcaaacat ttgattgccg atgctcaacg acagggtctg aagttggctg tgatctccaa
     1681 tgaatttggt gaattaggca ttgatcgagc cctgcttcaa gaagaaggcg gtccgggata
     1741 tgtggagctt gaaggcgggt gtgtgtgttg ccagctttcc ggtgagttgc tcaacaccct
     1801 gcagaatctg tgggaaacca ttcatccgga tcgggtcgtc gtggaaacat ccggtgtagc
     1861 attacccttc gaaacgcttc tgacgttttg gcgcgagccc gtcactgagt gggtagggga
     1921 aagtttagcc gtggtggtcg tcaatgccga acaagtggcc caaggtcggg atttggaagg
     1981 cacgtttgag caacaggtat cttcggcaga tttgttggtc attaataaag tcgatttagt
     2041 tccatcagat tccttcggtc gtttggaaga tcttctccat gatatggccc ctggcgctcc
     2101 agtgattcga agcattcagg gtgaaatcga tacgacgatt gtttttcctt ccattcctgc
     2161 ggcaaaaact tcatccaacg ctcaacgaaa gcccgaactc cttcctcaca cccatgagga
     2221 atttgaggcg gaggaactct cctttcccga caacatcacg cccgaacagc tccttaaaca
     2281 ccttcaacca ctcaacactc ttcgcataaa aggcatcgtg aacacgtcag aagggccgac
     2341 gcttgtccaa ggcgttggtc cccggattga tctcagtgcc cctccctcac aatttcctca
     2401 agaaatgctt ggccgtctgg tcgttatccg cagaaaatag agacttcggc cttgccaatc
     2461 tctccacgga tcgggttctc attcatgcag gacttgtgga aatcatcaac ttttattgtc
     2521 gactacccca aaaacacaca tggtgttgtc ttgacaccct tctatttagg gtatagcgtg
     2581 agtgtatgac gtatgaatct acgttgactc tcctatccct cgcg
//
LOCUS       DAORAQ010000316         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5479_length_2618_cov_70.914553, whole genome shotgun sequence.
ACCESSION   DAORAQ010000316 DAORAQ010000000
VERSION     DAORAQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_2618_cov_70.914553"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      <1..162
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            192..2219
                     /locus_tag="PKM72_14715"
     CDS             192..2219
                     /locus_tag="PKM72_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNP62096.1"
                     /translation="MFSGILRSCFLVVIFQIFGLLVIFLFFPIIVEAADSQHSESVPE
                     LDPMVVTATKTPVPLSQVTSAVEVITEESMQRRHSRTLVDAISLSQGVSTFSSGGPGT
                     NNTVRIRGGSSAQTLVLIDGAIVNSATLGEFNFGTVTTDNIESVTVLRGAQSMLWGSD
                     AAGGVVDIRTKRGTGEPVARIFSEYGSYNSIREGGSFAGQMGPVDFSATLTRWDMTKF
                     SSVNYRRGATERDAFRNWQASSLLGVKGPWDGRLELAFRWINNDIDLDNPSTFGGPFD
                     VFKAKSANEQFIYSGSYHQPITDWWDQKVTLAHAQERLITQAGDNQRSITTGLESTPG
                     AFGNSDIRTKSNRIEWQSNFKIGKPLLLTVGYQYREQIGENKGTFSEKKLSSNAGFAQ
                     LQLNLFDRFFATGGFRQDSYNTFGDATTYRVTGAYLLKETGTKLRTSYATGFRAPSIN
                     ELFFPNYGNSNLKPEKSQSFDIGIDQTFWDKRVTLSAGYFWNRYRQLIVTAFDPAGCA
                     PFSPFGFCAQNIGSAKSQGWEGSAKLILAEGLPYMKLLDLQGQYTYTITRDLETGSRL
                     PRWPVHQGSVVLTYQPIAPLSMTTTFRYVGSRFNTTGDQQPLPDFYLINVSASYDITP
                     SLQGYVRVDNILNRHYEEVLYFGTPVRSVFGGVRVTFDLPVSKTSSPTDNS"
     gene            2216..>2618
                     /locus_tag="PKM72_14720"
     CDS             2216..>2618
                     /locus_tag="PKM72_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNP62097.1"
                     /translation="MSFPRLVIAGTHSGVGKTTVTLALLSAFRAQGRTVQPFKIGPDF
                     LDPGHHQLASGRESRNLDGWMLGAVLNRTIFQEAAQDADLSIIEGMMGLFDGSSPVNA
                     TGSTAEMAHQLNAPILLVVDGSAMARSAAAMV"
BASE COUNT          618 a          606 c          730 g          664 t
ORIGIN      
        1 cgacggttgt gacggggaag ctggtgtaaa tccagcacgg tgccgccact gtaagcgggg
       61 agtaattctg caagagtcca ttgtggacct gatgtccatg agaaggcgca gaaacgcgaa
      121 gatccgcaag tcaggatacc cgacaatcag tctcttccat aaaacccctt cgtgcgaaag
      181 ggaggtggat catgttttca ggaattttgc gttcatgttt tctcgtcgta atttttcaaa
      241 tttttggatt gctcgtcata tttctatttt ttccaatcat tgttgaggca gcagattcgc
      301 aacattccga gtctgtcccg gaattagatc ccatggtggt gacggctacg aaaacacccg
      361 ttcctcttag tcaagtcacg agcgcagtgg aagtcatcac agaagagagc atgcagcgtc
      421 ggcatagtcg aacccttgtg gatgccatta gtttgagcca aggtgtatcg accttttcct
      481 ctggtgggcc agggacgaac aataccgtga ggattcgcgg agggagttcg gctcaaacgt
      541 tagtactgat tgatggtgcc attgtgaaca gtgcgacact tggggagttt aattttggta
      601 ccgtgaccac ggacaatatc gaatccgtga ccgtcctccg gggggcgcag agcatgctgt
      661 ggggttctga tgctgccggt ggggtggtcg acattcgaac caagcgcggt acgggtgagc
      721 cggttgccag aatcttttca gagtatgggt cctataattc gattcgagaa ggagggagtt
      781 ttgccggaca aatgggaccg gtagactttt ctgccacctt aacgcgttgg gatatgacca
      841 aattctcttc ggtcaattac cggcgtgggg caactgaacg ggatgcgttt cgcaattggc
      901 aggcgtcttc cctgttaggg gttaagggac cctgggatgg acgtttggaa ctggcgttcc
      961 ggtggatcaa caacgatatc gatctcgata atcccagtac attcggtggt cctttcgatg
     1021 tgttcaaagc gaaatccgcg aatgaacaat ttatttatag cgggagttat catcaaccga
     1081 ttactgactg gtgggatcag aaggtaaccc tcgcgcatgc ccaggaaagg ttaatcactc
     1141 aggccgggga taaccaacgt agcattacca cagggctgga gagtacccct ggagcgtttg
     1201 gcaattcaga tattcgaacc aaaagcaatc ggattgaatg gcagagtaat tttaaaatcg
     1261 ggaagcctct ccttttgacc gttggttatc aatacagaga acaaattgga gaaaataaag
     1321 gcacgttttc tgaaaaaaag ctttcaagta atgccggatt tgcacagctc caattgaatt
     1381 tgtttgaccg gttttttgcc accgggggat tccgacagga ttcctataat acatttggcg
     1441 atgcgacgac ctatcgtgtg acgggggcgt atcttctcaa agaaaccggc acgaaacttc
     1501 ggaccagtta tgccaccgga tttcgagcac cctcgattaa tgaactattt ttcccgaatt
     1561 atgggaactc gaatctcaag cctgaaaaaa gtcagagctt cgatatcggg atcgatcaaa
     1621 cgttttggga caagcgcgtt accttaagtg caggatattt ctggaaccgc tatcgccaac
     1681 ttattgtcac agcgtttgat cctgcaggct gtgccccatt ctcaccgttt ggcttttgtg
     1741 cacagaacat cgggtcggcg aaaagtcagg ggtgggaggg aagcgctaag ctcattctgg
     1801 ctgaaggcct tccctatatg aagttgctgg atcttcaggg acagtatacc tatacgatca
     1861 cgagggacct ggagacaggg tcccggctgc cgcgctggcc ggttcatcag gggagtgttg
     1921 tgttgaccta tcaacccatt gctcctttga gtatgaccac cacgttccgc tatgtgggtt
     1981 ctcggtttaa cacgaccggc gatcaacagc cactcccgga tttttatctc atcaatgttt
     2041 cggcctcgta tgacattacg ccctccttgc aaggatatgt gcgcgtggac aatattctga
     2101 accggcatta tgaagaagtg ctgtactttg gaaccccggt gcggtctgtg ttcggtgggg
     2161 ttcgagtgac gtttgatctt ccggtgtcaa aaacttctag tcccacggac aactcatgag
     2221 ctttccgaga ctcgtcattg cgggcacgca tagtggagtc ggcaaaacga cggtgacatt
     2281 agcgttgttg tcggcgtttc gcgcccaggg gcgcacggtt caaccgttta aaatcgggcc
     2341 ggattttctg gatccggggc atcatcagtt ggccagtggc cgtgaatcca ggaaccttga
     2401 tgggtggatg ctcggggcag tattgaacag aacgattttc caagaggcag cccaagatgc
     2461 cgacctctcg attattgaag gcatgatggg gctgtttgat ggaagttccc cggtcaacgc
     2521 aacgggaagt acggccgaaa tggctcatca actgaatgcg ccgatcctcc tggtcgtgga
     2581 tgggagtgcc atggcccgct cggcagccgc gatggtgt
//
LOCUS       DAORAQ010000317         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5502_length_2610_cov_86.679061, whole genome shotgun sequence.
ACCESSION   DAORAQ010000317 DAORAQ010000000
VERSION     DAORAQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5502_length_2610_cov_86.679061"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PKM72_14725"
     CDS             complement(<1..1286)
                     /locus_tag="PKM72_14725"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNP62098.1"
                     /translation="MVEVPLKLYVQALLKGAKEAARPMACISPSAKNQALLAMAESLV
                     DHTQEILEANAKDLAGISKATDQQAHREASERIRISEDTIHLMQQRLLDLQALPDPIG
                     EVSHAWLTTDGMQVHTVRSPLGIVAVVSDMGPLVTVESFGMCMKTNNVCVFRGGTEWF
                     HTNGAIVHYLQKAAVSNGVPEGGLTFLDRSSPEAALEMVRYPQYVQAVITRGSAGLRN
                     GIMEQSRVPVIGFEGGLCHVYVDQDVDLPLAQTIAVNAKLQSPFEANSMDTLLVHQGV
                     SRHLLPGLTRRLLQEFRVTLHGCPKTVSMLGVLEMSGHLGIKVAEETDWGKKYQSLTL
                     NIKVVKDSQEAIDHIGTYAPGHTDTIVTRDYQLAMRFVREVDSSAVMVNASTRLHGGP
                     QFGLGPDIGSNSTRMQGRGPLILSKLTIEKYMVLGTG"
     gene            1699..2481
                     /gene="rpsB"
                     /locus_tag="PKM72_14730"
     CDS             1699..2481
                     /gene="rpsB"
                     /locus_tag="PKM72_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNP62099.1"
                     /translation="MVTIKSLLEAGVHFGHQTNRWNPKMKRYIFGEKNGIYIIDLQIS
                     LQCFQKAYEFTRDTVAAGESVLFVGTKRQAMGIVEEEANRCGMFFVNQRWLGGMLTNF
                     QTLRRSINQLKKLEAAETDGTYERLKKKEIVRMKKEQAKLEKYLSGIKGMNDIPGCIY
                     ILDTRIQHITVKEANRLGIPVIALVDTNCDPEGVDFPIPGNDDAIRSLKLFTGQIADA
                     CIEGTEIRRKRQESVANGEGFLPDNPEQGQPSVAANQNSQPV"
BASE COUNT          730 a          655 c          571 g          654 t
ORIGIN      
        1 ccagttccca ataccatata tttttcaatc gtgagcttcg agagaataag aggacctcgc
       61 ccttgcattc gagtgctatt actcccgata tccggaccaa gaccgaactg aggtcctcca
      121 tgaaggcggg tcgaagcatt taccataacg gcgctcgaat caacctctcg gacaaagcgc
      181 atggccagct gataatcccg ggtcacaatc gtatccgtat gtcctggggc ataggtccca
      241 atatgatcga tggcctcctg actatccttc accactttaa tattcagggt caaggattga
      301 tattttttcc cccagtcggt ttcctccgcc accttaatcc ctaaatgccc gctcatttcc
      361 aacacaccca acatggaaac cgttttagga cagccatgca aggtcacccg aaattcctga
      421 agaagtcttc gtgttaatcc tggcaataaa tgccgagaaa cgccctgatg caccagaagg
      481 gtgtccatag aattcgcttc gaatggcgat tgaagtttgg cattgactgc aatcgtttgt
      541 gctaggggaa gatcaacatc ctgatcaaca tacacatgac acagacctcc ctcaaagcca
      601 atgaccggaa ctctggactg ctccatgatt ccattgcgca aaccagcgct ccctcgggta
      661 atcacagctt gaacgtattg aggatatcgc accatttcca gggctgcctc cggggaactc
      721 cggtcgagaa acgttaatcc cccttcgggg accccattgg aaaccgcagc cttctggaga
      781 tagtgaacaa ttgcaccatt cgtatgaaac cactcagttc ccccacgaaa aacgcagaca
      841 ttattggtct tcatgcacat cccaaacgat tcaactgtca ccagaggacc catatccgac
      901 accaccgcca caatacccag gggggaacgg accgtatgaa cctgcatgcc atcagtagtc
      961 agccaggcat gactcacttc tccgattgga tcaggaagag cctgtaaatc taacagccgt
     1021 tgctgcataa ggtgaatcgt gtcttctgaa attcgaatcc gttctgacgc ctcacgatgg
     1081 gcttgttgat cagttgcctt ggaaatgccg gccaaatctt ttgcattggc ctctaaaatt
     1141 tcctgagtat gatcgactaa actctcggcc atcgccagta acgcctgatt tttcgcacta
     1201 ggagatatgc atgccatggg gcgagcagct tccttcgctc ccttcagaag cgcttgcaca
     1261 tataatttca gaggtacttc aaccatcctt cgcactcctt catcatgaaa atgtttgaaa
     1321 accggattga ctatagccat gaagactcat ggagtcaaaa attgcattga ttcaaaaaga
     1381 ttttttacat gtccttcctt atattctacg atgcccgccg agggccaagc tcccgtcgca
     1441 tacctattaa aagataatca gggttgaatt agaatttcca cctatgctaa cctcgtttgg
     1501 tttaatggaa aattattaag catcaattaa attgcatgaa tgtctcttca cttggaacag
     1561 cctgcctgat aagacaatcc gcctttattg tctcctgccg gtcccatttg agccaaatta
     1621 ctgttttgaa tttcgttaaa aaatccactg tttcgtgtta aatcagtata tctctgaaag
     1681 ggggtgggaa ggtccgttat ggtcacgatt aaatcgttat tagaagctgg ggttcacttc
     1741 gggcatcaaa caaatcggtg gaacccgaag atgaaacgct atatctttgg ggaaaaaaac
     1801 ggcatttata ttattgacct ccaaatttct cttcaatgtt tccaaaaagc ctacgaattt
     1861 acccgagata cggtggctgc aggggaatcc gtcttattcg tggggacgaa gcggcaagcc
     1921 atgggcattg tggaggaaga agccaatcgt tgtggaatgt tttttgtcaa ccagcgatgg
     1981 ctgggtggca tgttgacgaa tttccagacc ctccgtcgca gcatcaacca actaaaaaaa
     2041 ctcgaagcag cagaaaccga cggcacatat gaacgactga agaaaaaaga aatcgttcgt
     2101 atgaaaaaag agcaagccaa acttgaaaaa tatttgagcg gcatcaaagg catgaacgat
     2161 attcctgggt gcatttatat cttagacacc cgtatccaac atattacggt caaggaagcc
     2221 aatcgtcttg gcattccggt tattgccctc gtggacacca actgtgaccc tgaaggcgta
     2281 gactttccta ttcctgggaa tgacgatgct attcgatccc tcaaattatt cacgggacaa
     2341 attgccgacg cttgcatcga aggtactgaa attcgccgga agcgacagga gtcagtcgca
     2401 aatggcgagg ggttccttcc agacaaccct gaacaaggtc aaccatccgt tgcggcaaac
     2461 cagaattccc agcctgtcta aggatcccgg gttcatcaat ttgaaccgaa atttataata
     2521 caatactcag taggagagca tgcatatgtc tagcttgagt gccttggtga aggagttgcg
     2581 aggcaaaaca ggggcaggaa tccttgattg
//
LOCUS       DAORAQ010000318         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5519_length_2603_cov_67.600078, whole genome shotgun sequence.
ACCESSION   DAORAQ010000318 DAORAQ010000000
VERSION     DAORAQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5519_length_2603_cov_67.600078"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKM72_14735"
     CDS             complement(<1..512)
                     /locus_tag="PKM72_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62100.1"
                     /translation="MRSTRLNLFSIMIAVVFSLCASLVFAGGATAKTGFSQAQESARK
                     WQADAVLVQIITVSGDMEGTSRKWSYLFYSPQAKKGYKVDVKDSKIDQALEVPSSFTD
                     AVDGDFIDSAQAITEGKKKGLKGKNRAMMTLHIMQQGTKSQGAYWNIVSDQADGRSTL
                     INAKTGKFFR"
     gene            complement(629..1480)
                     /locus_tag="PKM72_14740"
     CDS             complement(629..1480)
                     /locus_tag="PKM72_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNP62101.1"
                     /translation="MGEDKNQIVSGILRARAELEQVLYEMEKLPAISESAVHFAAHAL
                     NNYLTVTGGTVELLLLMLADHPDKQIRTGLESLQQATNLMAHTVSRLVNTETGLDAEM
                     RFEKVELPIMAQRFRLFYQRIADQKQIQCLLGSTGGVPPVWTDRVATAAALDNLFSNA
                     VKYSPPGKRIWVDVVPQQDWVVCRVRDEGPGLSQEDLAKLFQRGVLLTPRPTGGEPST
                     GYGLAVAKELIEKVGGEIWCESVVGQGACFSFRLPQYQELVHGSGRTLPGLHENKEHE
                     SRTVRHT"
     gene            2400..>2603
                     /locus_tag="PKM72_14745"
     CDS             2400..>2603
                     /locus_tag="PKM72_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNP62102.1"
                     /translation="MEIGIVGLPNVGKSTIFNALTSGNAAASNYPFTTIEPNVGVVAV
                     PDPRLARLTELVKPKKTVPTSCRF"
BASE COUNT          614 a          748 c          588 g          653 t
ORIGIN      
        1 tgtctgaaga acttccccgt tttggcgttg atgagggtac ttctgccatc cgcctggtca
       61 ctgacgatat tccaataggc gccctggctt ttggtgcctt gctgcataat gtggagggtc
      121 atcatcgctc gatttttccc cttgagccct ttctttttcc cttctgtgat cgcctgcgca
      181 ctgtcaataa aatctccatc caccgcatcc gtgaagctcg acggcacttc aagcgcttga
      241 tcaatcttgc tatccttcac gtccaccttg tacccctttt ttgcctgagg tgaataaaag
      301 agatagctcc acttcctcga cgtgccctcc atatctcctg atacggtgat gatttggacc
      361 agaaccgcgt ccgcctgcca tttcctggcc gattcctggg cttgtgagaa tccggttttt
      421 gccgtcgctc ctccagcaaa caccagtgat gcacaaagcg aaaataccac ggcgatcatg
      481 atcgaaaaga ggttgagtcg tgtcgatctc ataggaattt cccctttaaa ggtgttgcag
      541 agatggatgg cacgcccctg attgaactac aacaaaccgc ttacctgtcg aagaccatga
      601 acattgagta ttctgaataa ccctcagatc aggtgtggcg tacggtccgg ctctcatgct
      661 ccttgttctc gtgtaaaccc ggcaacgtcc gcccggatcc atgcaccagt tcctggtatt
      721 gaggcaatcg aaaggagaaa caggccccct gccccaccac gctctcacac cagatctccc
      781 cccctacctt ctcgatcaat tccttggcga cggccaagcc ataccctgtt gaaggctctc
      841 ctcccgtagg tcgaggggtg agtagtaccc cgcgctggaa gagtttggcc aggtcttcct
      901 gactcaggcc cgggccctcg tcgcgaacac gacataccac ccaatcctgc tggggcacca
      961 catcgaccca gattcgcttc ccgggtggtg agtacttcac ggcgttcgag aacaggttat
     1021 ccaacgcagc agcggtggcg acacggtcag tccacacagg aggaacaccg cccgtggaac
     1081 ccaaaagaca ctgaatctgc ttctgatccg caatgcgttg ataaaagagg cgaaacctct
     1141 gcgccatgat cggcaactcc accttttcga aacgcatttc ggcgtccaga ccggtctcgg
     1201 tattcaccag tcgactgacc gtgtgcgcca tcaaatttgt ggcctgttgc aaggactcaa
     1261 gcccggtacg gatttgcttg tccggatggt ccgctagcat caacaggagt aattcaactg
     1321 ttccgccggt cacggtgaga taattgttca gggcatgtgc agcaaaatgg acagcactct
     1381 cactgattgc cggcagtttc tccatttcgt acaacacctg ctctaactcc gcccgggctc
     1441 tgagaatgcc ggacacgatt tgattcttat cttcccccat gaacgctcct ccctgcgatg
     1501 gccatcgcga tcttctcgga gtaagtgaag cccaaacaac ttgagactga tccgcctaaa
     1561 ctgcggaatg ccgtcttctt caatatcatc agggtcattg ggatctcatg ataaatacag
     1621 gtgaacctcg tttgcatgac tatggtgcct caatagttgc cgcaatgcct ggctacattc
     1681 aatgatggga tcatcatcac atccccagga gcctagggct tgagacaatg tgccaatcag
     1741 cttctcctgc ccgcatcccg acacgcaaca gctacacagc ttgtgtcaat ttttcttcaa
     1801 acagcgaatt aagtcaagaa aaaacaagcc ggttgctaca cggttctgaa aaagagaaag
     1861 ccaaaggaac gacccacaat tgctcaccaa tttcgaggtc tgctgtgctt atgaaacacc
     1921 ccgttcgaat ccaggactgc cgggaatttt catacgaaac ctttacggca tcagttgatc
     1981 aacttactct tcaaaaatgg ccgtcattcc ctttctgcac atgccactta ccatgtttgc
     2041 tatggttgtc agctcctctc tcgatagggt cttcctgagg ccaggccgtt tcccggaatc
     2101 tctggacgac cgattgaagc ggatgtttta aaaacgatcc acaccgtttt tctttcggaa
     2161 acacgagggt gagtaagttc atcattttct attctgaaat cacctgaaaa ttgcgccttt
     2221 tctaagcctg aattgatcgg cacaaaactt tctatttcaa cctaaggaac tgatgcggtg
     2281 taaaccagag aaagattatt caccatccga aaatttttaa gagaagggaa cgaccggcta
     2341 cgtataggcc ttttggctca aggaccgaca ggttcctgat ttgttacctt gcgtcttcta
     2401 tggaaattgg tatcgtcggt ctacccaacg tcggcaaatc cacgattttt aacgcgttga
     2461 cctctggaaa cgcggccgca tcgaattatc catttacgac aatcgagccg aacgtcggcg
     2521 tcgtagccgt tcctgacccg cgtcttgcgc gcctgaccga gttggtcaaa cccaagaaaa
     2581 ccgtgccaac ttcctgccgt ttc
//
LOCUS       DAORAQ010000319         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5627_length_2569_cov_58.002784, whole genome shotgun sequence.
ACCESSION   DAORAQ010000319 DAORAQ010000000
VERSION     DAORAQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5627_length_2569_cov_58.002784"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKM72_14750"
     CDS             <1..266
                     /locus_tag="PKM72_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62103.1"
                     /translation="QDDNVQRDCVTLIVLPADVRRIHIDQCEPYQVVILSQRRRICAQ
                     GDESTAKPDSSFHLALSLDEWGRMTKSPQRDKISWVILRPFGE"
     gene            complement(695..2149)
                     /locus_tag="PKM72_14755"
     CDS             complement(695..2149)
                     /locus_tag="PKM72_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNP62104.1"
                     /translation="MPRLCLLGLDAGDFDYIQSRASALPCLQEKLKSGNLVKLDVPKA
                     LSGSVWPTFYNGADPGVHGMYQHLVWDAKRMGLRRIGADWSFYHPFWQDIENAGHHVV
                     ILDVPYSFPVALNKGLEITDWATHGQTYPLGCSQERVKAIIQDMGNSPIGRETPIQKT
                     SGELKTIQQQLIASAELKSRLIIELMQKVEWDLFVAVFAETHRGGHVFFSNEDEKAFG
                     PDTPLLKIYQAVDRALARIFGHVDEETTVVVFSAHGMTRDYAQGHIVRPLMKRINEIF
                     LEKYCGVSPKRTFRMNGLVPYLRNVVPARLQYAIGASSPDFVRQWVVEKEIIGGLDWS
                     LTPGFALRTDIRTELRLNLIGRESQGMLAPHSELHNHYVAFLKNVFLELRDRDTNVHL
                     VDEVVDTQEIFSGDRSGLLPDYVITWNARPFVKRVYSPLVGELTFAQLPGARGGDHTD
                     YGFAIIPDSVRDLHPLHHIKDLAGWSKRFVNMDG"
     gene            complement(2184..>2569)
                     /locus_tag="PKM72_14760"
     CDS             complement(2184..>2569)
                     /locus_tag="PKM72_14760"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HNP62105.1"
                     /translation="MMAKTGRDPSQLYLDLTKELGVPVYQRIDAPATPAQKAILKKLA
                     PQQIQASELAGEKITGILTSAPGTGSAIGGLKVMTENGWFAARPSGTEDVYKIYAESF
                     KGDTHLKQIQEEAQALITKVLKSAS"
BASE COUNT          615 a          636 c          646 g          672 t
ORIGIN      
        1 ctcaggatga caatgttcaa cgggattgtg tgactttgat tgttctccct gctgacgtta
       61 ggagaataca tattgaccag tgcgagccat accaggtggt catcctgagc cagcggcgaa
      121 ggatctgtgc ccagggtgac gagtcaacgg ctaagccaga ttcttcgttt caccttgccc
      181 tgagccttga cgaatggggc agaatgacaa aatctcctca gagggacaag atctcttggg
      241 tcattctgcg cccttttggg gaatagtcgg tgtccaggtc gaacgtcatc gcaactcagc
      301 tagatacttc gtttccccca tctctgaaag tgttttcaag tgttacgtct atgccggctc
      361 gtcgtcggcc gaattgccta tgcgaaaagg caacgttgcc gggaatcgtc aatggccccc
      421 agcgggggct tcaaagacac ccaaaagtga aacgtggggg ggaattctag agcgtattga
      481 atctttgcaa cggagatcat ttttcctcac cgaggcctct gaacgttcct tttcgacatg
      541 cgctcaagct cgattatggt aagggctgag ttgtgtttgt ctgaagccag tatccaccgc
      601 gactgggtaa gtcggattat gctcgcccga atcttctgac cacaacaccg ggtaggtggc
      661 ggtttgagtg agttgcgttg gcgggaggaa tggtttagcc gtccatattc acaaaacgct
      721 tactccagcc cgctaaatct ttaatatgat ggagagggtg aaggtcccgg acagaatcag
      781 gaatgatggc aaacccgtaa tcggtatgat cccctccccg ggcgccagga agttgtgcga
      841 atgtaagttc tcccaccagg ggagagtaga cccgtttaac gaatggccgg gcattccagg
      901 tgatgacata gtcgggaagc agtccactcc gatctcccga aaatatttcc tgggtatcaa
      961 cgacttcatc tacgaggtgt acatttgtat cccgatcgcg caattccaag aaaacgttct
     1021 tgagaaaagc aacatagtgg ttatgtaatt cactgtgggg tgccaacatt ccttgtgatt
     1081 ctctgccgat aagattgaga cgtaattccg ttctaatatc tgttcgcaag gcgaatccag
     1141 gggtcaatga ccaatctaat cccccaataa tttccttttc gaccacccat tgtctgacaa
     1201 aatccggtga gctggcaccg atggcatatt gtaatcttgc tggcaccacg ttacgtaggt
     1261 agggaacaag gccgttcatt cgaaacgttc gtttagggga aaccccgcaa tatttctcta
     1321 gaaaaatctc gttaatcctt ttcataagcg ggcggactat atggccctgg gcataatccc
     1381 tcgtcatacc atgagcggaa aacacgacga ccgtggtctc ctcgtctaca tgaccaaaaa
     1441 ttcttgctaa ggcccgatcg acggcttggt aaatctttaa taacggagtg tcagggccaa
     1501 atgccttttc atcttcatta ctgaaaaata catgtcctcc acggtgtgtt tctgcaaaca
     1561 cggcaacgaa taaatcccat tccacctttt gcattaactc tataatcaaa cggcttttta
     1621 gctctgcact ggcgatcagt tgttgttgaa tcgtcttcag ttctccggac gttttttgaa
     1681 taggggtttc tcgtccaata gggctgttgc ccatatcctg gatgatggct tttacacgtt
     1741 cctggctaca cccaagagga taggtttgcc cgtgtgtggc ccagtcggta atttctagac
     1801 ccttgttcag ggcaactgga aaactatagg gaacatcgag gatgacgaca tggtggcctg
     1861 cattttctat gtcctgccaa aacggatgat aaaaagacca atcagctcca atccggcgaa
     1921 gccccatacg ttttgcatcc cacacgaggt gctggtacat cccatgcacg cctggatcgg
     1981 ctccattata aaatgtcggc cagacggagc cactcaaggc cttcgggaca tcaagcttga
     2041 ccaggtttcc ggatttcaat ttttcctgta aacacggtaa ggcagaagct cgggattgaa
     2101 tatagtcgaa atcaccggca tccaaaccca gtaaacataa acgtggcatg gtcaacctaa
     2161 agatctttaa tacacggagt tgattacgat gctgatttca aaaccttggt aatcagggct
     2221 tgggcttctt cctggatttg cttgagatgt gtgtcgcctt tgaagctctc cgcataaatt
     2281 ttatagacat cctcggtgcc cgaagggcgc gcggcaaacc aaccgttttc cgtcatgact
     2341 ttcagcccgc caatggcact gcccgttccc ggtgccgagg tcaggatgcc ggtaatcttt
     2401 tccccggcga gttcggatgc ctgaatttgc tgtggcgcaa gttttttcag aatggccttc
     2461 tgcgcgggtg tggccggggc atcaatgcgc tggtagaccg ggacgcccag ttctttggtg
     2521 aggtcgaggt agagttggct gggatcgcgt cccgttttgg ccatcattt
//
LOCUS       DAORAQ010000320         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5659_length_2564_cov_65.836987, whole genome shotgun sequence.
ACCESSION   DAORAQ010000320 DAORAQ010000000
VERSION     DAORAQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5659_length_2564_cov_65.836987"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1098)
                     /locus_tag="PKM72_14765"
     CDS             complement(22..1098)
                     /locus_tag="PKM72_14765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNP62106.1"
                     /translation="MAEPINNRNIIDISPLPTPKQMQSSLSLPESTGQMVLQSRQAIR
                     DILHGQDSHRLLVIIGPCSIHDPDAAILYAERLKRVADRIQEHALIVLRTYFEKPRTT
                     VGWKGLINDPHLDGSCEIGGGFELARSILLRINNLGLPCATEFLDPVTPQYISDLISW
                     AAIGARTTESQTHREMASGLSMPVGLKNGTDGGLQVALNAMIAARHPQSFIGVNADGL
                     TSIIKTNGNPDRHLVLRGGGGRVNYNPEDISEAVRCLTKEGIRRPIMVDCSHGNSEKD
                     HTRQVPVARSVVEQFTNGQNAILGLLIESNLKPGNQKWEAGKPLEWGISITDACIGWE
                     ETEQLLDDLAETMDKSAKSKILTS"
     gene            complement(2265..>2564)
                     /locus_tag="PKM72_14770"
     CDS             complement(2265..>2564)
                     /locus_tag="PKM72_14770"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNP62107.1"
                     /translation="PSLPPKVTISSSPDGNSNVSAIGSNPAKDEGLLSLTPPPEVLSY
                     LERISQIPNVRQARIAQIQQAIDSQTYAIAAEKLADSLIQELHPHPQETRPATKS"
BASE COUNT          697 a          523 c          673 g          671 t
ORIGIN      
        1 cgttaaagag tatcaaataa gttaagaagt caaaatctta cttttagccg acttatccat
       61 tgtttcagcc aaatcatcaa gaagctgttc tgtttcttcc cacccgatac aggcatctgt
      121 aatagatatt ccccactcca ggggtttccc cgcttcccat ttttgattgc ccggcttcag
      181 attactttca attaataggc ctaggatagc gttttgccca ttggtgaatt gctcgaccac
      241 ggatcgagcc accggaacct gacgcgtatg atctttttct gaattgccat gagagcaatc
      301 aaccatgatc gggcgacgaa tgccttcttt cgttaaacaa cgcaccgcct cggaaatatc
      361 ttccggatta tagttgactc ggccaccgcc accacgaaga actaggtgtc ggtctggatt
      421 gccatttgtt ttgataattg aagtgagacc gtccgcattt acccctataa agctttgagg
      481 gtgtcgtgcg gcaatcatgg cattgagagc gacttggagc ccaccatccg ttccattttt
      541 caggccaaca ggcatggaga gaccactggc catttcccgg tgggtttgac tttcggtggt
      601 tcgtgctcca attgccgccc agctgattaa gtctgaaata tattggggtg tgacaggatc
      661 caaaaactcc gtcgcacagg gcaaccccaa gttattgatc ctgagtaata tagaacgggc
      721 cagttcaaat cctcctccaa tttcgcagga tccatctaaa tggggatcgt tgataaggcc
      781 cttccaaccc actgtcgtgc gaggcttttc gaaataggtt ctaaggacaa tcagggcatg
      841 ttcttgaatg cggtcagcaa cccgtttgag gcgttcggca tagaggatgg ccgcatccgg
      901 gtcatggatt gaacatgggc caataataac tagcaggcga tgtgagtcct gcccgtgaag
      961 aatatctcga atggcttgcc gggattgaag aaccatttgt ccggtgcttt caggcaagga
     1021 cagtgaactt tgcatctgtt ttggggtagg aagtggagaa atatcgataa tgtttcggtt
     1081 attaatgggt tctgccatgt gacacgtcct gggtaaaaag agagaatatc tgaaactgca
     1141 gaatgaaaga ccgtaaacaa attatgccgg aataggcgtt attgtaataa gaaaccctat
     1201 atccaggcaa ccagttaatg gcagtctgtc atactcctat tgtgaacagg gttggtagat
     1261 ttgctatcaa ggccgtgcaa agaggaaaag agtatgaatc atgtaagaat ccttgggatt
     1321 ggaatgggaa gttattggga gccccgggtt ctgtttgggg aatgaaagag ttcaattgag
     1381 gcttgatagc gcgtcaggca gtggtgttga tcagactggc ttgttgagag gtaaaccatc
     1441 gcatgacatc tcgattggcg gggtcctgaa gagagctaaa tgaaaatttg gtgccaataa
     1501 ctaattccgt tgtggggtca acccactcaa tagatgcgga aaattttgaa aagtggtcga
     1561 atcccggcaa acgacaactt atgtggagat gggccgaaga gacgagtggc catgcggaag
     1621 gggagacacg aaattgacaa cccgttggag atagattgca gagctgaaca ggcagtacag
     1681 cgaggattct gcctttgtca ctgagaggcc ggacggctcc agtcatattg accgtcaaac
     1741 gcggaaaagt gcgaggatgt tgtcgtgaaa cgttttgtgg ggcagctaat ctaatgatcg
     1801 gaggcaatgt gagtgtttct tggatgactg tttcaaattc ataaatgtct ccatcgatac
     1861 atgaacgacc tttacaagat gtcccacatt ttagtttgag gagtgccgga tcattaggca
     1921 gggcacagag gagggagtga tcggctccca gccccaataa ataggtttgg attaggaatt
     1981 tctctggtgt tgagaaaaag gtcaaccaaa ttgtgtttga ccctattgcc aggtaggaat
     2041 ttgacgtatt catgagatcc ttttcggtag cgtgaggtca tgttacaagc ccaaactcca
     2101 gcccgcaaga tgatataggg ataacaaaaa aataccaccc acaatgtaca ggaacaggtt
     2161 gaaaacttta caatattctc caaaagtgct catgcggcga cagagttttc caccaactcg
     2221 ggatgggaat gagtgaagca ctaggccgtt aagaggcgaa aaaattatga cttggttgcc
     2281 gggcgagttt cttgtgggtg aggatgaagt tcctgaatga gtgagtctgc taacttttca
     2341 gcagcaatag cataggtctg tgagtcaatg gcttgttgaa tctgtgcaat tctggcttgt
     2401 cgaacattcg gaatctgaga tatgcgctcg aggtaagata agacctctgg gggcggagtt
     2461 aaagataata gtccctcatc tttggctgga tttgatccta tagcggatac attgctgttc
     2521 ccatcaggag atgacgaaat tgtgacttta ggaggaagtg atgg
//
LOCUS       DAORAQ010000321         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5793_length_2523_cov_72.128444, whole genome shotgun sequence.
ACCESSION   DAORAQ010000321 DAORAQ010000000
VERSION     DAORAQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_2523_cov_72.128444"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKM72_14775"
     CDS             complement(<1..603)
                     /locus_tag="PKM72_14775"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP62108.1"
                     /translation="MQKKWEVVILGGGFAGLACARRLEKLWGNDTAKRVLLVSAENYF
                     VYQPFLSEVIGAGIEPRHVMNPIRLALRRCKVQRAEVTNIDLEKRRVEFNADEGASLE
                     PVEAEHLVIALGSSTNLRAVPGMMEHGLFLKTLADALGIREHIIKRLEEADLEPDPDI
                     RRSLLHFVVVGGGYSGVETAGEILDLLQDARGFYPAFDPSE"
     gene            1023..1397
                     /locus_tag="PKM72_14780"
     CDS             1023..1397
                     /locus_tag="PKM72_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNP62109.1"
                     /translation="MSIELNHTIVPAHDKVASAKFYAKIFGLVFDEGKVGYFAPLRIN
                     ETLTFDFDDDEVGTFDIHHYAFKVGEEDFDRIFGRIQAEGIPYGSGPSAREDMTINHR
                     GGGRGVYFCDQNGHILELLTVE"
     gene            1433..>2523
                     /gene="zwf"
                     /locus_tag="PKM72_14785"
     CDS             1433..>2523
                     /gene="zwf"
                     /locus_tag="PKM72_14785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNP62110.1"
                     /translation="MMGNALSDALVLFGATGDLAQKKIFPALQAMIQRGKIDVPIIGV
                     ARGGKDVDHLRQRARDSLEKFGGGVDEAAFAKLSDLLRCVDGDYQEEDTFTRLRQTLD
                     TAQRPLFYLAIPPSLFPAVVKSLGKSGCASGARVVVEKPFGRDLASAQALNGTLRSVF
                     DESAIFRIDHYLGKESIQNLLFFRFANSFLEPIWNRNYVESVQITMAEQFGVDGRGKF
                     YEETGAIRDVVQNHLLQVVANLAMEPPVSSGGEALRDERVKAFKGIRPLTGNSLVRGQ
                     FRGYREEAGVRPDSQVETFASMQLHIDSWRWEGVPFFIRTGKCLPVTATEVLVKLKHP
                     PHQLFDDRRSDASNHIRFRLGPDRVAIAMG"
BASE COUNT          552 a          659 c          702 g          610 t
ORIGIN      
        1 ctccgacggg tcaaatgcgg gataaaaccc tctggcatcc tgtaacaaat caagaatttc
       61 accagccgtt tcgaccccgc tgtatccacc accgacgacc acgaaatgga ggagagatcg
      121 gcggatatcg gggtcgggtt caagatccgc ctcctccaag cgtttaataa tgtgttcgcg
      181 gattcccagg gcatcagcca atgtttttaa aaatagcccg tgctccatca tgcccggtac
      241 cgcgcggaga ttggtgctac ttcccaacgc gatgaccagg tgttcggcct cgacgggttc
      301 caggcttgcc ccctcatccg cattgaattc gactcgtcgt ttttccagat caatgttcgt
      361 gacctcggca cgttgcacct tacagcggcg caaggctagt cgaatgggat tcatgacatg
      421 gcgcggctcg atacccgccc cgatcacttc cgaaagaaag ggttgataga cgaaatagtt
      481 ctcagcgctg accagcagca cgcgtttggc cgtgtcgttc ccccaaagtt tttcgagtcg
      541 acgggcgcag gccagaccgg caaatccacc accaagaatg acgacttccc attttttttg
      601 catacggcct atcgtctttt cttttgttga tcaggtgtcg tgattacgtc aatcttgtgc
      661 aggaacactt ctcatcgcca aaattttttc gttcgcccaa gatgctaccg agcttaacat
      721 gcttctactc ctcatcgccc ctcaagggaa ctgggaattt ccctggttgg gcagatgaaa
      781 gacccttgct gagtctcccg ttctgacctg ttagccgaga agatcctgtt tcagcttgcc
      841 gggccttcgc cgaaacagct agattttcct attgatgacc gtgtccagga agcacggtca
      901 aatgaaccat gggtcaacgt gtgagaggat agacaaaact ttcactgtgg ctcttcgcct
      961 gccggtcctg ccaccgcgag gccaggtgat tcccgaccat cgcgacgata aggagatatg
     1021 ccatgtcgat tgaactcaat cacactatcg tcccggcgca tgacaaagtg gcatcggcaa
     1081 aattctatgc gaaaattttc gggctcgtgt tcgacgaagg aaaggtcgga tatttcgcgc
     1141 cgctacggat taatgagacg ctcacgtttg atttcgatga tgatgaggtc ggcaccttcg
     1201 acattcatca ctatgcattc aaggtcggtg aagaggattt cgaccggatc tttggtcgca
     1261 ttcaggccga aggcattccc tatgggagcg ggccaagtgc gcgggaggat atgaccatca
     1321 accaccgggg tggcggacgc ggagtatact tttgcgatca gaacggacac atccttgaac
     1381 tccttacggt ggagtagggt gggatcaatt gttttgatgg tttagggagg gaatgatggg
     1441 gaatgctctt tcagatgctc ttgtgctttt cggggccaca ggcgacctcg cacagaagaa
     1501 aatttttccg gcgcttcagg ccatgattca acgtgggaag atcgacgttc cgattatcgg
     1561 tgtcgccaga ggcggcaagg atgtggacca cctccgacag cgtgccaggg acagtctgga
     1621 aaaattcggc ggcggggtcg atgaggccgc ctttgcaaag ctttccgatt tgctcagatg
     1681 tgttgatggg gattatcagg aggaagatac gtttacccgt ttacgtcaga ccctagatac
     1741 cgcgcaacgc cctctcttct atttggccat ccctccgtct ctgtttccag ccgtggtgaa
     1801 aagcttagga aagtcaggct gtgcctccgg cgcacgagtc gtcgtggaaa aacccttcgg
     1861 gcgcgatctg gcttccgccc aagccctgaa tgggaccctg cgcagcgttt ttgatgaatc
     1921 ggcaattttc cgcatcgatc attacctcgg aaaggagtcg attcaaaatc tcctgttttt
     1981 tcgttttgcc aattcgtttc ttgagcccat ctggaaccgc aactacgtcg aaagtgtgca
     2041 gatcacgatg gcggagcagt tcggggtaga cgggcgtggc aagttttatg aggaaaccgg
     2101 tgcgatccgg gacgtcgtgc agaatcactt attgcaagtg gtggccaatc tggccatgga
     2161 gccacctgtc agttccggtg gggaagctct gcgcgatgaa cgggttaagg ccttcaaggg
     2221 cattcggcca ctcacgggaa attccctggt gcgggggcag ttccggggct atcgtgagga
     2281 agccggggtg aggccggatt cgcaggtaga aacctttgcc tcgatgcagc ttcacatcga
     2341 ttcgtggcgg tgggaaggcg tccccttttt cattcgaaca gggaaatgcc ttccggtgac
     2401 ggccacggaa gtgttggtca aactgaaaca tccgcctcat cagctctttg acgacaggcg
     2461 gtcagatgca tcgaaccata ttcgtttccg gttgggtccg gatcgagtcg cgattgcgat
     2521 ggg
//
LOCUS       DAORAQ010000322         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5826_length_2513_cov_67.591945, whole genome shotgun sequence.
ACCESSION   DAORAQ010000322 DAORAQ010000000
VERSION     DAORAQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5826_length_2513_cov_67.591945"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PKM72_14790"
     CDS             complement(<1..1493)
                     /locus_tag="PKM72_14790"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNP62111.1"
                     /translation="MKAEAENLEFVTINGLLTYGEAIARRPPVEGAEPHSPLTSLEDR
                     PQRTLEAMAAIRLFVQEAQKGFANAAAYREARQALIRQACGGDELVFFAAWNQLLAQG
                     ELAPLFRAPIGATNKPIRRRPVAIVPREHMTPNLAEGRIVLDIGDDRFWLMPRDLSAR
                     TLFFTMRHGVSRMDSKKFRVGRRLRNVLDAERGIPKADAIGTALVRTLGLVGKQLDFL
                     QLDNYLDSKSFVHMVSQSPNTRQLFERVVSILSPETAEATQPFTEWALESQDFGWATG
                     IEKTIEIEEAARAFGVDTKMAQRLIKHPLYSYPGGHSFFELYVELVDGFHQLGQSHQG
                     KVLCLYTHSSTLRALLIFLDPRPFSEAFSEFGAYKEGQDNVVLLTYEHGQLSGYSTAV
                     GLSERERAVREALMTAEQGRRGKVILKPRQIRRIVALVSGGDFAGAGAALKELRVTGN
                     RLGLEVYFVQHGFLGLANNWIELVTEQDTRGMSNHASSPIGSSRFED"
     gene            1952..>2513
                     /locus_tag="PKM72_14795"
     CDS             1952..>2513
                     /locus_tag="PKM72_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNP62112.1"
                     /translation="MQKTKIICTIGPVTESYEMLRKMYDAGMNIVRLNMSHGTHESHA
                     KVIKHIKTLNTKVPFPIPILLDTQGPEIRTGDLSIDLNLQEGTIVSISARGPMDVEES
                     SIHINYADLMESVNVGDKITVDNGLINFEVLEKQDRLMQCRVLDGGVLKSKRHVNLPG
                     IRVNLPAITQKDEKDIAFGLAADVDFI"
BASE COUNT          635 a          700 c          592 g          586 t
ORIGIN      
        1 aaatcttcaa accggctgct gccaatcggg ctgctggcat gattgctcat gccacgtgtg
       61 tcctgttccg tcaccaactc aatccaatta ttggccaacc ccagaaatcc gtgttgcacg
      121 aaatagactt cgaggcctaa ccggttcccc gtcacgcgga gctccttcaa tgccgctccg
      181 gctcccgcaa aatccccacc ggagaccagg gcaacgattc gccgaatctg gcgtggtttc
      241 aggatgacct ttccccgacg gccttgctcc gcagtcatca aggcctcgcg gacggcacgt
      301 tcccgctctg aaaggcccac ggcggtggaa tagcctgaca attgcccatg ttcataggtg
      361 aggaggacga cgttgtcctg tccttcctta taggcgccaa attctgagaa ggcttcactg
      421 aatggacgag gatccagaaa aattaagagc gcacgcaagg ttgagctgtg cgtatataaa
      481 cacaacactt ttccctggtg gctttgaccc aactgatgga acccgtcaac caattccaca
      541 tagagttcaa agaaagagtg accgccggga taactgtaca gggggtgttt aatcagacgt
      601 tgcgccatct tcgtatcgac cccaaaggcc ctggcggctt cttcgatttc aatcgttttc
      661 tcaataccgg tggcccatcc gaagtcttgc gattctaagg cccattccgt gaatggctga
      721 gtggcctcgg ctgtctcggg agacaggata gagacgacgc gttcaaaaag ctgccgggta
      781 ttgggactct gactcaccat atgcacaaag gatttcgaat ccaggtagtt atccaactga
      841 aggaagtcca gttgttttcc caccaagcct aaggtacgga caagggcggt tccgatggcg
      901 tcagccttgg gaattccccg ctcggcatcc aggacgtttc tcaaccggcg ccccacccga
      961 aacttcttac tatccatccg ggagacaccg tgccgcatgg tgaaaaacag cgtgcgggca
     1021 cttaaatccc ttggcatcaa ccagaagcgg tcatcgccaa tatccaggac gatccgaccc
     1081 tccgccagat tgggcgtcat atgctcgcgc ggaacgatcg ccacgggtcg acgtcgtata
     1141 ggtttattcg tggccccgat tggtgctcga aacaagggag caagctcgcc ctgagcgaga
     1201 agctgattcc aggcagcgaa aaacaccaac tcatctcctc cgcaggcttg ccggatcaac
     1261 gcctgccttg cctctctgta agctgcggca ttggcaaacc ctttttgagc ctcctgaaca
     1321 aacagacgta tggcggccat ggcctccaat gtccgttgcg gacgatcttc caatgaagtt
     1381 aaaggggaat gaggctccgc accctctacg ggcggacgtc tggcaatagc ctccccatag
     1441 gttaacagcc cgttaatcgt gacaaattcc agattttctg cttctgcttt catctattca
     1501 cctcaccaaa tccttcagct attattggtt tcatacccat cttgcatcat cataatatga
     1561 ttcgacttta tcgacaacgc acaccctcct ccataaacat ttgagtcatc tcctgtcgat
     1621 acaatgatcc tgtgcatccg gttctcccgc ctcgtgggaa aaacgccctt cgttcactca
     1681 cccggagggt gaaaacacac catcacaatg gagtcccgac cggtgatcca ccatgctaga
     1741 gtttaacgct gtagtgggct acacttcata agtacccaca gggaggtggg atatcttcca
     1801 cgccaagcag cactccaacg acacgaatac cgacaatccg atgaacctca accggaaact
     1861 ggggcaccca atcccttttt gacacccggg tttaggatga tgagatcgtt tccaccattc
     1921 acgatcatga aaaactgata gggacccatt catgcaaaag acaaaaatca tctgcacgat
     1981 cggaccggta accgaatctt atgaaatgtt gcgcaaaatg tatgatgccg gcatgaacat
     2041 cgtgcggttg aacatgtccc atggaaccca cgaatcccat gccaaagtga tcaaacatat
     2101 taagacgctc aataccaagg tcccatttcc tattccgatt cttttagata cccaaggccc
     2161 tgaaatccgg acaggggatt tatcgattga cctcaatctt caggaaggca cgatagtttc
     2221 tatttctgcg cgtggtccga tggatgtgga agaaagctct attcacatca actacgccga
     2281 tttaatggaa tctgtgaatg tcggcgataa gattaccgtg gataacgggt tgattaattt
     2341 tgaggttctg gaaaaacagg atcgtctcat gcagtgccgg gtccttgatg ggggggtatt
     2401 gaaaagtaaa cgccatgtca atttacctgg aatccgcgtc aatctccctg ccattaccca
     2461 aaaggacgaa aaagatattg cctttggttt agccgccgat gtggatttca ttg
//
LOCUS       DAORAQ010000323         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5835_length_2510_cov_66.635845, whole genome shotgun sequence.
ACCESSION   DAORAQ010000323 DAORAQ010000000
VERSION     DAORAQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_2510_cov_66.635845"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKM72_14800"
     CDS             complement(<1..664)
                     /locus_tag="PKM72_14800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNP62113.1"
                     /translation="MSKTLKKNSLVLTEGPDRAPARAMLRAVGLTDEDFTRPLIAIAN
                     TWSEITPCNYHLRDLAASVKQGIREAGGTPIEFNTIVVSDGISMGTEGMKASLISREV
                     VADSIELVVRGHLFDGVIALSGCDKTIPGCVMALARLNLPSIMLYGGSIMPGEFHGKP
                     VSIQDVFEAVGSHAKGKVTTEELIELERRACPGAGACGGQFTANTMSIAFEFLGISPM
                     GFN"
     assembly_gap    1210..1309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1310..2074
                     /locus_tag="PKM72_14805"
     CDS             <1310..2074
                     /locus_tag="PKM72_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62114.1"
                     /translation="VKDMSSLIYQGGNRKVWWISLTVWGVVTVLPVWGAGESRSEGGL
                     ENELSSGVENLEPFQKEKVNVSFADIYKQRVPGYVFLAHSEIASSPQPSPDIVSKGDG
                     SALVGSVMALLATFGDANVLPPEGTSQANQLIHGLIQLQSALVKSQSSELSEYFIAAV
                     EQRFDKEGAGILQSIYQRGLTSKMLDALVSYNHKIPMWEKPALTELFQRYNVSRQDWE
                     LIEKIFGEADVAYRRKGSSIHDAYELWRAQMPGGRP"
     gene            complement(2086..>2510)
                     /locus_tag="PKM72_14810"
     CDS             complement(2086..>2510)
                     /locus_tag="PKM72_14810"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNP62115.1"
                     /translation="VRVLTQDEIHSVSADISRPGPDDPENRAFIKLVTQKGLPCYLDI
                     SRVSQGRVTRAEIMGSYGQAFADWTTNIVRKISMGNEILDHSCPPSATLIEVLRDFCQ
                     AIRTGGPMPVTAEDGLRAVKLADACYRSAQTGKPVFLN"
BASE COUNT          608 a          549 c          635 g          618 t
ORIGIN      
        1 cattgaatcc cattggggaa attcctaaaa actcaaacgc aattgacatg gtattcgccg
       61 taaattgacc accgcacgcg ccggctcctg gacaggctcg gcgttctaat tcaatcagct
      121 cctccgttgt cactttccct ttggcgtgtg atccgacggc ttcaaagaca tcctggatcg
      181 acacaggttt tccatggaat tctccgggca tgatggagcc tccatagagc atgattgatg
      241 gcagattcag acgggccagg gccatcacgc atcccggaat ggttttgtcg caacctgata
      301 aggcaatgac tccgtcaaac agatgtcctc gaaccactaa ttcaatggag tctgccacca
      361 cttcccggct aatcaaggag gctttcatgc cctcggtccc catgctgatg ccgtctgaga
      421 ccacgatcgt attgaattcg atgggggtcc ctcccgcctc acgaatgcct tgcttcacac
      481 tcgccgcgag atcgcggaga tgataattgc aaggagtaat ttcagaccag gtattggcga
      541 tcgcgataag gggcctggta aagtcttcgt cggtcaagcc gaccgccctg agcatcgcac
      601 gagcgggggc acggtcgggt ccctcagtca ggaccaggct attttttttc aaggtttttg
      661 acatgaaacg gtgctccttc gatcataacc gttgatgaat ggaatattgc gtcagccaat
      721 gattcgatgc ctgaatgtct cataatgcta ggaaaatggt caatgttata gggccaaaat
      781 attgcacgta ttttagccat tagcgatcat gctttcccat gaaatcagat ggagaagaga
      841 agaggatggg cggtgagtaa cgccggacag tcctttcctc tgaaatggaa agaggaggga
      901 ggtcttgatc ttgaaattcc cgggaggtgt gtttacgagg tgtcaccgat gaaaggctac
      961 gttgtagccg gaacttccat ccattcctgg ggatgaggga gtaaacaata tgagccaata
     1021 aggattggag aaatgatagg tctcaccatg acggtaaagc tctacaagct gtcgtaatct
     1081 ggattgcttc aactaatttc gcaggggctt tgattccatg gccccttctt gaaggtattc
     1141 caagagtcga tcatgccgga ttgaaatgct gtgggtgcag gattttctca aaaattaatt
     1201 atcgcttttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tgaaagacat
     1321 gagttcgttg atctaccagg gcggcaatcg taaagtgtgg tggatctccc tgactgtatg
     1381 gggcgtggtg actgtcctgc ccgtgtgggg agctggtgaa agtagaagtg aaggaggctt
     1441 ggagaatgaa ctctcatcag gagtcgaaaa tcttgagcca tttcagaagg agaaggtaaa
     1501 cgtttccttc gccgatatct ataaacagcg ggtaccgggt tatgtatttc tcgctcacag
     1561 cgaaattgcc tcgtcgcccc agccttcgcc ggatattgtt tcaaagggag atggttcagc
     1621 tttagttggt tccgttatgg ccctgttagc cacgtttggg gatgccaatg ttcttccgcc
     1681 cgaggggacc tctcaggcca atcaactgat tcatggcctc atccaattgc agtcggcttt
     1741 agtgaaatcc caatcttcag agttgagcga atattttata gcggcggttg agcagcgttt
     1801 tgataaggaa ggtgcaggga ttttacagtc aatttatcag agagggttga catcaaaaat
     1861 gttagatgcc ctcgtgagtt ataaccacaa aataccaatg tgggaaaagc cagctcttac
     1921 tgaactcttt caacgttata atgtttcccg gcaagattgg gaattaattg agaaaatctt
     1981 tggcgaagcc gacgtagcct atcgtaggaa aggatcatcc atccatgatg cctatgagct
     2041 gtggagagca caaatgccgg gaggtcggcc atgaagatac aggaattaat tgaggaaaac
     2101 gggtttcccg gtttgtgctg atcggtaaca cgcatcggca agtttgactg ctcgtaaccc
     2161 atcttccgca gtaacgggca tcggtcctcc ggttcgaatc gcctgacaaa agtctcgaag
     2221 tacttcaatt aacgtggcac tgggagggca ggaatggtct aatatctcat tgcccatgga
     2281 aatttttcga acgatgttgg tcgtccaatc ggcgaatgct tggccatatg accccataat
     2341 ttctgcgcgg gtgactcgtc cctggcttac gcgtgaaata tcgaggtaac agggaagtcc
     2401 tttttgcgta accagtttaa tgaaagcccg attttcgggg tcatcgggcc caggtcgact
     2461 aatatcagcc gagaccgaat ggatctcatc ctgagtgagg acacggacca
//
LOCUS       DAORAQ010000324         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5865_length_2502_cov_63.681242, whole genome shotgun sequence.
ACCESSION   DAORAQ010000324 DAORAQ010000000
VERSION     DAORAQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5865_length_2502_cov_63.681242"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKM72_14815"
     CDS             <1..872
                     /locus_tag="PKM72_14815"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNP62116.1"
                     /translation="LNAYSSTSQQVDTWLEAYHRAKELCGSKTRPPASEVEQASLDGL
                     RRGVFAKRLIKKGRELTRDLVYFAMPYVEGQMESGAWKEGYTAVQDMTPDQPVMRDAV
                     EITVNQGLVTLKQAIHEVKALLNQANIQLGSEFKVEYSHHYGLENFRQTGAVLIECIN
                     REYCKKLVIQLPGQRHPSHYHARKEETFQILFGILHVNIDGYPRILHPGETILILPGV
                     WHSFWTDTGVVFEEVSTTHYNNDSFYADKRINKLQRSERKTMVDHWGRFQIAQQPSSE
                     KAPEVPLPDPQAQ"
     gene            869..1552
                     /locus_tag="PKM72_14820"
     CDS             869..1552
                     /locus_tag="PKM72_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNP62117.1"
                     /translation="MIACVAFDFDGTLVDSNQVKVQSFYKIVEEYDPSGCTVTEVLQR
                     CSQNDRYGITRELAREFKAKGLIPPPTSTEILGLQWAETYTATCETAIVRCQEVPGAS
                     GILTWLLNEGIPLYLNSKTPTNALNRLVTLRNLTHYFSGIYGAPASKLENLRHIQELT
                     QAKPDEMLFVGDSEDDLEAAGKFGCHFAGVILGENSRFRLIPSLHVTSLDNLRGIVQN
                     LQENGNAPT"
     gene            1677..2384
                     /locus_tag="PKM72_14825"
     CDS             1677..2384
                     /locus_tag="PKM72_14825"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNP62118.1"
                     /translation="MIGTQRILAVCPARGGSKGIPLKNLTPFLGIPLVVRVGQLVKEI
                     PFIDRAVVSTDHPEIAECAKKSGLDAPFFRPPDLSGDRISDAPVLIHALEEMEHQDNV
                     QYDIVIMLQPTSPLRRPEHVIQAIEMLVNEQWDAVWTLSETDSKSHPLKQLTLDSGRI
                     DYYDPNGSQIIARQQLTPVYHRNGVAYVMTRNCLLEGRSIMGQRTGALLLDEDLVSID
                     TMWDLELAEYIYAKKQA"
BASE COUNT          689 a          676 c          586 g          551 t
ORIGIN      
        1 aattgaatgc ctactcgtcc acctcacagc aagtggacac ctggctggaa gcctaccatc
       61 gggcaaaaga attgtgtggc tcaaaaaccc gtccaccggc ctcagaagtg gaacaggcct
      121 cattggatgg tcttcgacgt ggagtctttg ccaaacggct cattaaaaaa ggccgggaac
      181 tcacacggga tcttgtgtat tttgccatgc cctacgtcga agggcagatg gaaagcggag
      241 cctggaaaga gggctatacg gccgtccagg acatgacgcc ggatcaaccg gtcatgcggg
      301 acgcggtcga gatcacggtc aaccaaggtc tcgtgaccct caagcaagcg attcacgaag
      361 tcaaagcctt gctcaaccaa gccaacattc aactaggcag tgagtttaaa gtcgaatatt
      421 cgcatcatta cggacttgaa aatttccgac aaaccggcgc agtccttatt gaatgtatca
      481 atcgggagta ttgcaaaaaa ctcgtcattc aattgccggg acagcgacac ccctcacatt
      541 atcatgcgag gaaagaggaa acctttcaaa tactgtttgg catcctccac gtgaatatcg
      601 acggctatcc ccgcatttta catcccggtg aaaccattct catcctacct ggagtgtggc
      661 attccttttg gacagacacc ggggtggttt ttgaagaagt gtccaccacc cattacaaca
      721 acgactcctt ctatgccgac aaacgaatca ataagctgca acgatccgag cgaaaaacca
      781 tggttgatca ttggggacgg tttcaaatcg cccaacagcc atcatcggag aaagctcctg
      841 aagtgccttt gcccgatcct caggcgcaat gattgcttgc gtcgcattcg attttgacgg
      901 aacgctggtt gattcaaacc aggtgaaggt tcagtccttt tacaagattg tcgaggaata
      961 cgatcccagc ggttgcactg tcactgaagt tcttcagcga tgctctcaaa atgatcgata
     1021 cggaatcacc cgtgaacttg cccgcgagtt caaggcgaaa gggctaatcc cgccacccac
     1081 aagcacagaa atcctcggct tacaatgggc ggagacctac acagctacct gcgaaaccgc
     1141 cattgtcagg tgccaggaag tacctggggc ctcagggata ctgacatggc tgttgaatga
     1201 agggattcct ctatacctca actcaaaaac gcccaccaac gccctcaatc gacttgtgac
     1261 cctacgaaac ctcacccact atttctcggg gatatacgga gctcccgcaa gcaaactgga
     1321 aaacttgcgt cacatccaag agctgactca ggcaaaacca gatgagatgc tttttgttgg
     1381 agacagtgag gatgatctgg aggctgctgg taaatttggc tgtcactttg cgggagtcat
     1441 tcttggtgag aacagccgct tcagactgat cccatcactc catgtgacaa gtctggataa
     1501 tctcagagga atcgtgcaaa accttcagga aaacggaaat gcacctacct aaaaccattg
     1561 agcatctact cgcaccattg cagttgaaaa ttctttacac ttttgccaca ggaagttatg
     1621 atttacaccc aaccgatggc tatatcaaca atgcggtata tccctaaaaa aacccaatga
     1681 taggtacgca acggattctg gctgtgtgtc cagccagggg cggcagcaaa gggattccgc
     1741 tcaaaaatct gactcccttt ctaggaatcc cgttagtcgt cagagttggc cagttggtta
     1801 aggagatccc cttcatcgac cgagccgtag tctcgaccga ccatcctgaa attgccgagt
     1861 gtgcgaaaaa atctggccta gacgcccctt ttttccggcc tcccgacctt tcaggggacc
     1921 gaatatccga cgccccggta ttgatccatg cattagaaga aatggagcat caagacaatg
     1981 tccaatacga catcgtgatc atgctgcagc caacatctcc tcttcgccgg ccggaacacg
     2041 tcatccaggc catcgaaatg ctggtgaatg aacaatggga tgccgtctgg acgctgtccg
     2101 aaaccgattc caagtcacac cctctcaaac aactgactct tgactcaggc aggatcgact
     2161 attatgaccc caacgggagc caaatcattg cccggcagca actcactccc gtctatcacc
     2221 ggaacggggt ggcctatgtt atgacacgaa actgccttct ggaaggaagg agcatcatgg
     2281 gtcaacgaac tggagccctc cttctcgacg aggatctggt cagtatcgat accatgtggg
     2341 atttggaact tgcagaatac atctatgcca agaaacaggc ataacatctg aatccagtca
     2401 agatgaggat gaaccatctg ctaatcagag gtcatctcgc aggtcaaatg gctcggttgg
     2461 ctatgaacac cccctatgtt cagcccctga cgaaccaacc ag
//
LOCUS       DAORAQ010000325         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5869_length_2501_cov_65.897383, whole genome shotgun sequence.
ACCESSION   DAORAQ010000325 DAORAQ010000000
VERSION     DAORAQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5869_length_2501_cov_65.897383"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..830)
                     /gene="lptE"
                     /locus_tag="PKM72_14830"
     CDS             complement(144..830)
                     /gene="lptE"
                     /locus_tag="PKM72_14830"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNP62119.1"
                     /translation="MRWLLSGWGVVLLCLSAGCGYQFTVEGPGPVVGDSADHVSQGPP
                     VRLVFPVLKNNSFEPNLEFKYTRYLRQAFQSVGSAEFVDDASADFIMEGAILSVSLPS
                     LAFTRTQTQESRVMVRVVVRVKDRKTGRVRWSQAGTSTAEFFVGAKSTSDSETGLQFN
                     RVLQDRAVEQAGIQVAADLADQFLGARDQGVFDRNRNDAVPDPESSEDESLDNSAPQG
                     DFLPPGSQMP"
     gene            complement(862..>2501)
                     /gene="leuS"
                     /locus_tag="PKM72_14835"
     CDS             complement(862..>2501)
                     /gene="leuS"
                     /locus_tag="PKM72_14835"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNP62120.1"
                     /translation="IWVANFVLYEYGTGAIMAVPAHDQRDFEFAQTYHIPIRLVIQDP
                     EGKLASETLEAAYVEQDDAGLLVNSGNFSSLSVSQAKQAIGEYVETQGWGKRTINFRL
                     RDWGISRQRYWGTPIPMLYCDRCGIVPVPEGDLPVLLPDDVPFTGKGGSPLKESPSFA
                     QATCPTCGGPARRETDTMDTFVDSSWYFLRYCSPKDTTQAVNPQAAQHWMAVDQYVGG
                     IEHAVLHLLYARFFTKVLRDLGLTTADEPFTHLLTQGMVTKDTYWCEEHRWLLPTEIK
                     KNGEQRVCIHCGQAIVTGRTEKMSKSKKNIASPEDLCDRYGADTARMFSLFAAPPEKD
                     LEWSDTGVEGCYRFLNRVWRLVQDLLPVMNGAASNQGSGESVLLPQLQRATHQTIKKV
                     TEDLERGFQFNTAIAALMEFVNELYKFWKEHPGETLTPAERAEWRTTMETLVMLLAPF
                     APHVTEELWEILGKRPGMSRQAWPKFDPVLVASAEWTIPLQVNGKLRSKIVVPAGSTK
                     EQIITEAQADPKLVEWLQGKVARKIIYVEQKLVNFVI"
BASE COUNT          604 a          738 c          586 g          573 t
ORIGIN      
        1 cctgatcgcg aaaatagggc tcttccccaa tcaccaaata cagaggggat aacccttgcc
       61 ggttgatctg actttgaagt tcatgaactt tcatggtgaa tgcgcccaaa gcaggagaga
      121 aacctgccaa accattccgt taatcaaggc atctgggaac ctgggggcaa aaagtctccc
      181 tgaggcgctg agttatcgag ggattcgtcc tcactggatt ccggatccgg gacagcgtca
      241 tttctattcc tgtcaaaaac cccctgatcc ctggccccca aaaattgatc ggctaagtcg
      301 gcagcaacct gtatccccgc ttgttcaacg gcccggtcct gcaagacccg gttaaattgc
      361 aatccagtct cggaatctga ggttgatttg gcccccacaa agaactctgc cgtactggtt
      421 ccggcttgag accatcggac tcttcccgtt ttccgatcct tcaccctcac caccaccctc
      481 accatcactc ggctttcttg tgtctgcgtg cgggtaaagg ccaacgaagg aagcgagacc
      541 gataggatcg cgccttccat aataaaatcg gcggaggcat catcgacaaa ttcggcgctt
      601 ccgaccgatt gaaacgcctg acgaaggtac ctggtgtatt tgaattccag gtttggttca
      661 aagctgttat tttttagaac aggaaagacc aaacggaccg gcggtccttg cgaaacatgg
      721 tcggcactat ccccgaccac cggacccggc ccctcaacag tgaattggta cccacatccg
      781 gcactcagac acaggagcac gacaccccat ccgctcagca gccatctcac ccgcttaaga
      841 agcctacctc gtaggagggc gttaaatgac aaaattcacc agcttctgtt ccacgtaaat
      901 aattttgcgg gcgaccttgc cctgcaacca ttccaccaat ttgggatccg cctgagcctc
      961 tgtaatgatt tgctcctttg tcgaaccggc tggaaccacg atcttactcc ggagttttcc
     1021 attcacctgc aaaggaatcg tccattcggc gctggccact agaacgggat caaatttggg
     1081 ccaggcctgc cggctcatcc ctggccgctt ccccaggatt tcccacaact cctccgtcac
     1141 atgtggagca aagggtgcaa gcaacatcac caaggtttcc atcgttgttc gccactcggc
     1201 tcgttcggca ggggttaacg tctcccccgg atgttctttc cagaacttat acaactcatt
     1261 cacaaattcc atcagagccg caatggccgt attaaactga aaaccccgct caagatcctc
     1321 cgtcaccttc tttatcgtct gatgcgtggc ccgctgcagt tgagggagaa gcacggactc
     1381 gccggatccc tgatttgaag ccgccccatt catgaccgga aggagatcct gcaccagccg
     1441 ccagacccgg ttgagaaaac ggtagcatcc ttccactccc gtatcgctcc attccaaatc
     1501 cttttccggt ggggcggcaa ataacgaaaa cattctggcc gtgtccgccc catatcggtc
     1561 acacaagtcc tcaggggagg caatattctt tttagacttg gacattttct cagtgcggcc
     1621 tgtcacaatg gcctgcccac agtgaataca aacgcgctgt tcaccatttt ttttgatttc
     1681 agtgggaagc agccatcgat gctcttcaca ccaataggta tccttggtca ccatgccttg
     1741 cgtaagcaaa tgggtgaagg gttcatcagc cgtcgtgaga cccaggtcac gtaatacctt
     1801 ggtaaagaag cgtgcataca acagatgcaa cacggcatgc tcgattccac cgacatattg
     1861 gtccacagcc atccaatgtt gtgcggcctg gggattcacc gcttgtgtgg tgtctttagg
     1921 cgagcaatag cgaaggaaat accatgacga atccacaaag gtatccatgg tatcggtttc
     1981 acggcgtgcc ggaccgccac atgtgggaca ggtggcttgt gcaaacgaag gagattcctt
     2041 aagtggagac ccacccttcc cggtgaacgg cacatcatca ggcaacagca ccggcaagtc
     2101 gccttcaggg accggaacaa tgccacaccg atcacaatac agcatcggaa tgggcgtgcc
     2161 ccaatagcgt tgtctggaga tcccccaatc tcttaaccga aaattgatgg ttcgttttcc
     2221 ccatccctgt gtttccacat attcgccgat ggcctgtttg gcttgcgaca cggacaagct
     2281 ggaaaaattc cctgaattca ccaaaaggcc tgcgtcatcc tgttctacat aggcggcctc
     2341 cagggtttca gaggccaact tgccttccgg gtcttggatt accagccgaa tcgggatgtg
     2401 ataggtttgt gcgaattcaa aatctcgctg gtcatgcgcg ggaacagcca taatcgcccc
     2461 cgtgccatat tcatacaaca caaaattcgc cacccaaatc g
//
LOCUS       DAORAQ010000326         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5887_length_2495_cov_59.796311, whole genome shotgun sequence.
ACCESSION   DAORAQ010000326 DAORAQ010000000
VERSION     DAORAQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5887_length_2495_cov_59.796311"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKM72_14840"
     CDS             complement(<1..277)
                     /locus_tag="PKM72_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP62121.1"
                     /translation="MGIILLPPAFAEQEQAQPGKALFLQYCQDCHGPQRDGRGALRPF
                     LEQDPANLTSQMTQTKTDQELFSIIKKGRGEMHGWADTFTDEQVLDLV"
     gene            complement(448..897)
                     /locus_tag="PKM72_14845"
     CDS             complement(448..897)
                     /locus_tag="PKM72_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNP62122.1"
                     /translation="MRVATLFVIILPAVLGLPSMWMSEAKAQSSPSPQNDTAIMGTSE
                     SPTVSSEQTLKDDPVCDPTYRPTITKIEPDEFQAGAKIVIMGEHFGKKKDCLHEVTFG
                     PVKATEFTLQGDDKIEVTVPDSVATGMLFVSVVTGGGAAKTAVLVKK"
     gene            complement(1023..1388)
                     /locus_tag="PKM72_14850"
     CDS             complement(1023..1388)
                     /locus_tag="PKM72_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNP62123.1"
                     /translation="MKSRIMLWSLILLIGWAIPSGAQEPQADLFQGEAVYQAHCVRCH
                     GVQGKGDGPDAASLIVPPANFQRMESRAKSETDLRSAIIWGLAFSPMHGWWDTLSIED
                     IRAVTAYIRRIAPYEPSSP"
BASE COUNT          571 a          575 c          646 g          703 t
ORIGIN      
        1 gcaccaggtc caggacctgt tcatcggtga aggtgtccgc ccagccatgc atttcgcccc
       61 ttcctttctt gataatggaa aagagttcct ggtcggtttt ggtctgagtc atttgcgagg
      121 tcaaattggc tgggtcctgt tccaggaaag gacgtaaggc gcctctgccg tcccgctgtg
      181 ggccatgaca gtcctggcaa tattggagaa agagcgcttt gccgggttgg gcctgttcct
      241 gttcagcgaa agccggggga aggaggatta tacccatggc tgtgatcagg agaacaaagc
      301 acatcaatgg gcccaagcgt ctcatggaaa aaagattccg gaatccacca aatatcctta
      361 ggggcagaaa aggaatgtgc accgtcccgg tcgagttcgc tatcttatcg ccctgaatgg
      421 tgcacgggcg aatgtttgtt cccttgacta tttcttgacc agtacggcgg tttttgccgc
      481 acctcccccc gtcaccacgc tcacaaataa cattccagtg gcgacgctgt cgggcacggt
      541 tacttctatt ttatcatcac cttgaagggt gaattccgtt gcctttacgg gtccaaacgt
      601 gacctcatgc aggcaatctt ttttctttcc gaaatgttct cccataatga ctattttagc
      661 gcctgcctga aactcatccg gttcgatttt ggtgattgtg ggtcgatagg tcggatcgca
      721 tactggatca tctttgaggg tttgctcact ggacaccgta ggcgattcgg atgtgcccat
      781 aatcgcggtg tcgttttgag gggaagggct tgattgggct tttgcctcgc tcatccacat
      841 cgagggtaat cctaaaacag ctggcagtat gataacgaat aatgtcgcga ccctcatata
      901 gtaatccttt cagaaaaaaa tgagaataaa gccgcaccgt ttgagttagg cgtattaaag
      961 cctgtttgaa tccacgaaag ctagcccccg gaaaaatcag tgatcctctt tcgggggtaa
     1021 ttttagggcg atgaaggttc ataaggcgct attcgtctga tgtaggcggt gacggcgcga
     1081 atatcttcga tgctgagtgt gtcccaccat ccgtgcattg gactgaatgc cagcccccag
     1141 atgatcgctg accgaaggtc cgtctccgat ttggctcggg attccatgcg ttgaaagttg
     1201 gcgggtggca cgatgagcga ggccgcgtcc ggtccatcgc cttttccctg gaccccgtga
     1261 catctcacgc agtgggcttg atacacggcc tctccttgga agagatcggc ttgcggctcc
     1321 tgggctccac tgggtatggc ccacccgatc agcagaatta aactccaaag catgatgcga
     1381 gatttcatat ggtgtctctc cttgtgagtg tccatgcggt atgatcgagt gaggcatggg
     1441 atcggacggg gttcatcatt atttttagtt tgagatatct tgggtttccg gcgcgggcgt
     1501 gccggggggt tggttccctg ccattcgact ctagccattt agtcttgatg cgaccattgg
     1561 taggtgatct tctctttgaa atcccgataa agatatttgc ccaggcgttg ggcaaagtgt
     1621 tcggtggtcg tgtcggtatc cgccccgtca gttctttatt cttgtacaaa atggaacgcc
     1681 tttgcctttt gggtatcgtt atcatctttg cctcgttttt atgatgatta cattactctg
     1741 taaactgcat tcctataaat ggataaatcc aattttcatg ccgtaaaggg gaaccgaatc
     1801 gttctggata gaattaatgg aaaaagaccc acacagggtt tttgaaagtt gacttgctgg
     1861 ttggatagga aggcggatta aaactgtacc atcatgcccc atgccggaag agttcatccg
     1921 tcccactttt ccccaaaatc aagttccagg tggtgaagga tttctggaga aattgaatta
     1981 ggaaatggtt cccaacgtcc tgtcgcctaa tttccatgag aggatatacc caccatcgcc
     2041 ttggattgca ggcttcagtt tttccacggt atcaagatgt gtcgtgaggg ctatagcttg
     2101 gtcgttgtgg agggctgtgg tctttgagct gtcttgattt gtgcgaaggg cgcaatctta
     2161 ttggattaat atctcattca ctgttaaaaa gagccgatta atcagttccc cagataagga
     2221 accaacgcct gttgccacta aagctgacaa cagaatgctg atgaaaatga tgaatagaaa
     2281 tctcatgacg ttctcttttt ctggggttat cctgctttat ccaattatta tttgatgact
     2341 tcagtgtgcc tttcaatcat tctcttgaga gccaatagac gatgtgttct ccacatgatc
     2401 tcagatgtca tggagtgccg cagtggttgc aatttatagc tggagtaaga ccgtcatgaa
     2461 tatgactcaa aggaagggga atcacggtcc atggc
//
LOCUS       DAORAQ010000327         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5940_length_2481_cov_73.501649, whole genome shotgun sequence.
ACCESSION   DAORAQ010000327 DAORAQ010000000
VERSION     DAORAQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_2481_cov_73.501649"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1463
                     /gene="mnmE"
                     /locus_tag="PKM72_14855"
     CDS             9..1463
                     /gene="mnmE"
                     /locus_tag="PKM72_14855"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNP62124.1"
                     /translation="MGSLDDTICAVATPMGEGGVGIVRVSGVRALPVASSILQLRSGK
                     SLEQIKPYQLYLGQFIWNPIQSGENEGRAIPSVLDEVLVVTMRAPRSYTGEDVVEVHA
                     HGGPVIVNAICEALTQTGARLAEPGEFTKRGFLNGRMDLTQAEGVLDTIRANSLQSLK
                     IAQEHLQGRLSLLIQGQRDRLVKLVAHLEAGMDFVDDDIQFIDQSELKQDTQEILGEI
                     LQLLESAEEGRMIREGIRTVIVGRPNVGKSSLLNGILGTNRAIVSHIPGTTRDVLEEA
                     IMVEGVMIRLFDTAGLRETTDELENEGMHRANQAIEDADVLIVSFDGSLPPNDKDLSF
                     IEKYIQKPCVMVLNKIDLPAKFSLKELQSIVNQNRISSEDGFVGEKKYVEVSALTGEG
                     LDRLKRAIKSLAVGRQFEANDSVLVSRLRHKTLLHNAGVALKNAIVAIDDGLSAECVA
                     LELRMALNSLGEIVGAITNEDILDQIFREFCIGK"
     gene            1508..>2481
                     /locus_tag="PKM72_14860"
     CDS             1508..>2481
                     /locus_tag="PKM72_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP62125.1"
                     /translation="MSRECDIVVVGGGHAGCEAALAAARMGCRVVMLTIDLKKIATMP
                     CNPAVGGIGKGHLVKEIDALGGEIGWNTDQSGIQFRVLNMRKGPAVRATRVQCDKQIY
                     ANRMQDTVSRQDNLSIIQGTVDRILTQGGAVNGVKTNSGETILTKAVVLTSGTFLKGL
                     MHIGLNHLPGGRAGEPSAENLSDCMRDFGFEIGRLKTGTPPRIDRDSIDFNVMIPQPG
                     DLVPRPFSIRTTTLPNPQIHCHITYTNDETHRIIRENLDRSPMYCGIIESTGPRYCPS
                     IEDKVVRFADKNRHQIFMEPETLESRSFYPNGISTSLPVDVQQAFVRT"
BASE COUNT          682 a          518 c          650 g          631 t
ORIGIN      
        1 tctaagctat gggctcttta gacgacacca tctgtgccgt ggccacccct atgggagaag
       61 ggggtgtagg aattgttcgg gttagtggtg ttcgtgccct tccggttgcc tcaagcattc
      121 ttcaattgcg ctctggtaag tctcttgaac agattaagcc ctatcagttg tatttgggcc
      181 aatttatttg gaaccctatt caatctgggg aaaatgaggg aagagcgatt ccctcggtat
      241 tggatgaggt cctggttgtg accatgcgag ccccccgatc ctatacagga gaagatgtcg
      301 tagaagtcca tgcacatgga gggccggtca ttgtgaatgc catttgcgaa gccttgacgc
      361 agactggtgc cagattggca gaacctggtg aatttaccaa gcggggcttt ttgaatgggc
      421 ggatggattt aacccaagca gagggagtct tagataccat acgagcaaat tcccttcaga
      481 gtttgaagat tgcccaggaa caccttcagg gaaggttatc tctgctcata caaggtcaac
      541 gggaccgtct cgtgaaactc gttgcccacc tggaagccgg aatggatttt gtggatgatg
      601 acatccagtt tattgaccag agcgaattga aacaggatac acaggagatc ttgggagaaa
      661 tattgcagct gttggagtcg gccgaagaag gcagaatgat tcgtgaaggt attcgtaccg
      721 tcattgtagg gcgacccaat gtgggtaagt ccagtttgtt gaatggcata ctgggaacca
      781 atcgagctat tgtgtcccat attcctggga ccacacgtga tgttctcgaa gaggccatca
      841 tggttgaagg agtcatgata cgtctctttg ataccgccgg attacgagaa accacagatg
      901 aattagagaa cgagggaatg catcgcgcta atcaggccat tgaagacgcg gatgtgttga
      961 ttgtgtcatt tgacggaagt ttaccaccta atgataaaga cttaagtttt attgaaaaat
     1021 atatacaaaa accctgtgtc atggttctca ataaaatcga cttgccggca aaattttccc
     1081 tgaaggaact tcagagtatt gtcaaccaaa atagaataag ctctgaggat ggtttcgtgg
     1141 gagaaaaaaa atatgttgaa gtatcggcct taaccggtga aggacttgat agattaaaga
     1201 gagccatcaa gtccttggct gtggggcgcc aatttgaagc caatgattca gtgctcgtat
     1261 caagactgcg acataagacg cttcttcaca acgcgggtgt ggccttgaaa aacgccattg
     1321 tagccataga tgatgggctt tctgccgaat gtgtggcatt agaattacgg atggcactga
     1381 attctcttgg agaaattgtc ggcgcgatca cgaatgaaga tattttagac caaatttttc
     1441 gagagttttg cattgggaag taaaaggcat tccggccaat tttttcaaaa aaatagaata
     1501 ctaactcatg agtagggaat gtgacattgt cgtcgtcgga ggaggtcatg ccgggtgtga
     1561 ggctgccctt gcagccgcca ggatggggtg tagagtggtg atgttaacta tagatttaaa
     1621 aaaaatcgcc acaatgccat gcaatcctgc tgtcggaggg attgggaagg gccatctcgt
     1681 taaagaaatc gatgccctcg ggggagaaat aggatggaac accgaccagt ccggcattca
     1741 gttccgtgtg ctcaatatgc ggaagggacc ggccgtccgc gccacacgag tccaatgtga
     1801 caagcaaatt tacgccaatc gcatgcagga tacggtaagc aggcaggata atttgtcaat
     1861 tatccaaggc accgttgatc ggattcttac gcagggcggg gccgtcaatg gtgtgaagac
     1921 caatagcgga gaaacaattc tcacgaaagc ggttgtcctg acttcgggaa cctttctgaa
     1981 gggacttatg catattggat tgaaccatct tcccggcggg cgagcgggcg agccttctgc
     2041 ggagaatcta tcggattgca tgagagactt tggttttgaa attggacggc taaaaacagg
     2101 aacccctccg aggattgatc gggattcaat tgattttaac gtcatgattc cgcaacctgg
     2161 ggatttagtc cctcgaccat tttccattcg aaccactacc cttcccaacc cgcaaattca
     2221 ctgccacatt acttatacga atgatgaaac acatcgaatt ataagggaaa atcttgatcg
     2281 gtctccgatg tactgcggaa tcattgaatc aacaggtcca cgatattgtc cctctattga
     2341 agataaagtg gtcaggtttg cggataaaaa cagacatcaa attttcatgg aacctgaaac
     2401 tcttgagtca cggtcatttt atccgaatgg aatttcaacc agcctgccgg tagacgtcca
     2461 gcaggcattt gtccggacta t
//
LOCUS       DAORAQ010000328         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5950_length_2479_cov_67.352723, whole genome shotgun sequence.
ACCESSION   DAORAQ010000328 DAORAQ010000000
VERSION     DAORAQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5950_length_2479_cov_67.352723"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKM72_14865"
     CDS             <1..904
                     /locus_tag="PKM72_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNP62126.1"
                     /translation="SFIDFCCRIDSQKVNKRVLEGLIKVGAFDSMGLTRASTFQVLDE
                     ALEHASTIQRNKREGQTSLFEILAPEPATPKQDGFGFVIPQIPEWSQNDLLKYERELT
                     GFYITAHPLNRHSVGITHFSTCSTQTIRDVGDGREVKVCGVISSLKITTTKKGNRMAY
                     AQLEDLHGTVEAIIFPETFKQHEELLGPESVVRISGTVDLMDNGARIKATKVESLSQL
                     ENETVKHVTLQVHEQDVLPHNLLDLKDIFERHRGPTPISLAFHLHPDLTASMSGLSDI
                     GISPSPEFLEEVEHLLGTSTVTFQ"
     gene            930..1919
                     /locus_tag="PKM72_14870"
     CDS             930..1919
                     /locus_tag="PKM72_14870"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNP62127.1"
                     /translation="MREYLDFEKPLKELEERIAKLVKSKSKKVSVQEAIVKSTEQLAN
                     LEREIFANLSPWQHSQIARHPQRPSISDYLEHMTEGFMELHGDRLFGDDRAIMGGFAT
                     WKGRSIMAIGHEKGKSLKERMRRNFGMAKPEGYRKALRLMKLAERFNRPIITFIDTPG
                     AYPGVDAEQRGQAEAIARNLLEMSRLQVPILAVVTGEGGSGGALALGVADRILMLEHA
                     IYSVISPEGCAAILWGDASKSADAATALRMTGPELRKLNIIEEVIPEPMGGAHRDPKT
                     MADRISQALDTHFLQLQKLSPAALRQQREAKFRSLGTFGSSQATPKQVKTVNS"
     gene            complement(2198..>2479)
                     /locus_tag="PKM72_14875"
     CDS             complement(2198..>2479)
                     /locus_tag="PKM72_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62128.1"
                     /translation="FNRCWALMHESPPPVKTQERVLDLTLSDEITLEALVHVIRQTFE
                     EHGFVELTWDHPPSEPRRESTPEAGPLVERLRQWEPPSDKLNPPSSSSN"
BASE COUNT          640 a          649 c          621 g          569 t
ORIGIN      
        1 ctcctttatt gatttttgtt gccgcattga ttcgcagaag gtcaacaaac gggtgttgga
       61 aggactcatt aaggtcggtg cctttgattc tatgggtctc acgcgcgcca gcacgtttca
      121 ggtcctggat gaagcccttg agcatgcctc gacgattcaa cgcaataaac gggaaggcca
      181 aaccagtctg tttgaaatat tggctccgga accggccacg ccaaagcaag acgggtttgg
      241 tttcgtcatt ccacagattc ccgaatggtc tcagaatgac ttactcaaat atgaaagaga
      301 gctgaccggt ttttatatta ctgcgcatcc actcaaccgc cacagcgtgg ggatcactca
      361 tttttctacc tgctcgaccc agacgattcg cgacgtcggc gacggacgag aggtcaaagt
      421 ctgcggggtc atttcaagcc tcaaaattac cacgacgaaa aaaggcaaca ggatggccta
      481 tgcccaattg gaagatttac acggaaccgt tgaagccatt attttccctg aaacctttaa
      541 gcaacacgaa gaattattgg gaccggaatc ggtcgttcgg atatcgggta ccgttgacct
      601 gatggataac ggcgcccgca tcaaggccac caaagtcgaa tcgctctccc aattagaaaa
      661 tgaaaccgtg aagcatgtga cgctccaggt ccacgaacag gacgttctcc cccataatct
      721 gctagactta aaggacatat tcgaacgtca ccgtggtccg acccctattt cgttggcgtt
      781 tcacttgcat ccggatctga ccgccagcat gtcaggattg tcggacatcg gcatttctcc
      841 gtccccggag tttttagaag aagtggaaca tctcctcggc acatccacgg tcaccttcca
      901 ataatttccc tctaccaagg agcctgtccg tgcgtgaata cctggacttt gaaaaacccc
      961 tgaaagaact tgaggaacgc attgccaagt tggtgaaatc aaaatctaaa aaggtttcgg
     1021 ttcaggaggc catcgtcaaa tcaaccgaac aactggccaa tcttgagcgt gagatctttg
     1081 ccaatctctc gccatggcaa catagccaga tcgccagaca cccacagcgc ccttcgattt
     1141 ctgattatct ggaacacatg acggaaggtt tcatggaact ccatggtgat cgcctgttcg
     1201 gtgacgaccg cgcgatcatg ggagggtttg ccacatggaa gggccgctca atcatggcga
     1261 tcggtcatga aaaaggaaaa tcattgaagg aacgcatgcg ccggaatttt ggtatggcca
     1321 aaccagaggg ttaccgaaaa gcgttgcgtc tcatgaaatt agcggaacgc ttcaaccgac
     1381 ccattatcac ctttatcgat actccaggag cataccccgg cgttgatgcg gagcagcgcg
     1441 gccaagccga agctatcgcc agaaatttgc tggaaatgtc cagattgcag gtgccgattc
     1501 tggctgtggt cacaggagaa ggcgggagtg gtggagcgtt ggccctggga gtggcagatc
     1561 gtattctgat gctcgaacat gccatttatt cggtgatttc tccggaaggc tgtgccgcaa
     1621 ttctgtgggg ggacgcctca aaatctgccg atgccgctac cgccttacga atgacgggac
     1681 cggagttacg caaacttaat attatcgagg aggtcatacc cgaaccaatg ggaggtgccc
     1741 atcgggatcc aaaaactatg gccgaccgta tctcgcaggc actggatacc catttcctgc
     1801 aattacagaa attgtcgccg gcagcgctcc gacaacaacg ggaggccaaa ttcaggagcc
     1861 tgggaacctt tggctcttct caggccacgc caaaacaggt aaagacagtc aactcgtaac
     1921 ctgcatgaag tcagtaacca tttccaagct accaagggcc cacaggatac cttttttttg
     1981 attggttcgc acgttctcca tgcgattgtt catctccccc cattagacac cacgaagtcc
     2041 acgtattgca gtccgattat ttttttgcgc tccagacaaa cgccaccatt gaccgatcgc
     2101 catagctcag agggtgattc tccgctgggc aggaaagatc ttcatccgcc agaaagggga
     2161 accggtccaa gacatcttcg gggatcagga tggaggacta gtttgatgaa gatgatggag
     2221 gattcaactt gtctgaagga ggttcccact gtcgtaaccg ctcgaccaaa gggccggctt
     2281 cgggagtcga ttcgcgtctg ggttcacttg gtggatgatc ccaggtcaat tcaacaaagc
     2341 catgctcctc gaaggtctgt cgaatgacat ggaccaaagc ctccagagta atctcatcac
     2401 taagcgtcaa atcaaggacc cgctcttgtg tcttcacagg cggagggctt tcatgcatta
     2461 atgcccaaca ccgattgaa
//
LOCUS       DAORAQ010000329         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_5978_length_2470_cov_61.171429, whole genome shotgun sequence.
ACCESSION   DAORAQ010000329 DAORAQ010000000
VERSION     DAORAQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_2470_cov_61.171429"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PKM72_14880"
     CDS             complement(<1..1322)
                     /locus_tag="PKM72_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62129.1"
                     /translation="MSVEPFGVLEHLKVLSPNHVYFLASKAFVLRGYEYYAQGRLESY
                     SWDRTQTILSATVRGATQYLVRFGVHNGQLTFSCTCPVWTPESHCKHVICALLTTVNL
                     LLPDTFRMPSSNSTQRALLLRQLMERVGSPLASKPPTPAAPPRFEITLRNRHGVSSIS
                     ITNHGNICQSFAGMPTELAVLLRATQDPAWSVQEGLRDFLKHHGQNHALFFEHADGKY
                     PVEWAPDALYTTKTELDILGSHVSIAACCLRFGEVQRDTHACMGFAADLGAKKLGPLE
                     HEGGWAVYDLLYENSQRERVIEPEDVLSRPLALPSTSHNRDGWLSSERPTGTDRPTFS
                     IPVDQFMALQFDIPLHEQDRILRSVLLKCAGQEVSLTDHRHSGETPIYQYRLTFIPET
                     IPMDASVLDLRTGILRAECWLGESKGRPSIPIFRMFPFFENAKTVSSG"
     gene            complement(1902..>2470)
                     /locus_tag="PKM72_14885"
     CDS             complement(1902..>2470)
                     /locus_tag="PKM72_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNP62130.1"
                     /translation="TWKLNAKNCLLHIQRSTDGEGVPTTILVVDDGLFNGVVSHAVFP
                     KVDEVVVTAREGQRCEIQPSQDEFLVIARAPRAPLASEKEWANVPIGGVVVLFRATTF
                     YLPHDVTIMPGQKVLWIYADGAQEPHSVTSGGCRVNDCSGGGKQFDSRLNLNKPGQRF
                     EHVFKHPGTFPYHCDLHLGSMQGTVIVK"
BASE COUNT          656 a          650 c          613 g          551 t
ORIGIN      
        1 aacccgctcg agaccgtttt ggcattctca aaaaatggaa acatcctgaa aatagggata
       61 ctgggcctcc ccttgctttc cccaagccaa cattccgcgc gtagaatccc tgtccttaaa
      121 tccaacaccg aggcatccat cggaatggtc tctggaatga atgtgagacg atactgataa
      181 attggcgtct ctccggagtg gcgatgatca gtgagtgaga cttcttgacc ggcacacttg
      241 agcaataccg accgcaaaat tcgatcttgt tcgtggaggg gaatgtcgaa ttgtaaggcc
      301 ataaactggt ctaccggaat ggaaaaagtt ggtctgtccg ttcctgtggg gcgttccgat
      361 gaaagccaac catcgcgatt gtgggatgtc gaaggcaggg ccaacggacg gctgagaaca
      421 tcctcaggct caataacccg ttcccgttga ctgttttcat acaacaaatc gtacacggcc
      481 cacccacctt catgttcgag tggtccaagt tttttcgcgc ccaaatcagc cgcaaagccc
      541 atacatgcgt gggtgtcccg ttgcacctct ccgaaacgca aacaacaggc ggcaatcgag
      601 acatgcgaac cgagaatgtc caactcggtt ttcgtggtat ataacgcatc cggcgcccat
      661 tccacaggat atttcccatc cgcatgctca aagaacaagg catgattctg tccgtgatgt
      721 ttaaggaagt cacgcagccc ttcctggacc gaccatgcag gatcctgggt ggcacgaagc
      781 agcacggcta gctcggtggg cattcccgca aaggattggc aaatgttccc atggttggta
      841 atgctaatgg atgacactcc atgacggttt cgaagagtaa tctcgaagcg tggaggagcg
      901 gccggagtgg gtggttttga ggccaaaggg ctcccgacgc gttccatgag ttgtcgcagg
      961 agcagcgctc gttgggtgga attggatgaa ggcatacgga aggtatcggg aagcaggaga
     1021 ttgactgtgg tcaatagggc acaaatgaca tgtttgcaat gcgactccgg cgtccacacc
     1081 ggacaggtgc aggaaaaggt cagctgcccg ttgtgcacac cgaaccgaac cagatactgg
     1141 gtcgcccctc gcaccgtagc cgacaagata gtctgcgtcc ggtcccagct atacgactcg
     1201 agccttcctt gggcgtaata ctcatacccg cgaaggacaa aggccttgga agccagaaag
     1261 tatacatgat taggagaaag aactttcagg tgttccagga caccaaaggg ttctacggac
     1321 atcgtccccc ccccgttcta tgggaccctg gccaatcaat acacaggatc ggcttcagac
     1381 accaggacat tcccggtcgt gatggacaac gcatcactcg gcagcagcgc agataagaca
     1441 tatggatgtt ctataaaaca caggcaccgg tcgaccaaac acccccggtt gatgtcacga
     1501 attgaacggg ttttcccact ccagatgcgt atcggccaaa acgctaatta tacaccactt
     1561 atcctctgat gtatgcccat ccggaccggg tagatgaaaa aacgctggac cgtctcaatc
     1621 ccgcaaaaaa cgtttcgtga aaagaagatc aatcctttgt tctgaagggg agaacacctt
     1681 ttggccagaa gcctcaaaaa ttttaagcca agccataccg cggtacaaaa tggtgagtat
     1741 tcaacgcgga tgcctctggt attcttatgc acccatggga agattcatct gtgggattat
     1801 cagcctgcag aaaaaacatt tttccatcaa ccccgattat cctcaggctt tcactcgtgc
     1861 aggcatagag gaaggagggt tagttaatgg attggaaaag cttatttgac gatcacggtg
     1921 ccctgcatag atcctaaatg aaggtcgcaa tggtagggaa atgtaccggg atgcttaaac
     1981 acatgctcga accgctgtcc gggtttattg agattcaatc tactatcgaa ctgttttcca
     2041 ccaccagagc aatcgttcac ccgacagcca ccacttgtga cactgtgggg ttcttgggcg
     2101 ccatcggcat aaatccacaa gaccttttgg cccggcataa tcgtgacgtc atgaggtaaa
     2161 taaaatgttg tagcccggaa caggaccacc acccctccaa tcgggacatt agcccactct
     2221 ttttcggaag ccaggggagc acgtggagcc ctggcaatca cgagaaattc atcttgagac
     2281 ggctgaattt cacacctctg cccttcccga gccgtgacca ccacttcgtc tactttagga
     2341 aagaccgcat gggaaacaac tccattaaat agaccatcat ccaccaccaa tatggtggtg
     2401 ggtacacctt caccatccgt agatctttgt atatgcaaaa ggcaattttt cgcattcagt
     2461 ttccaggtca
//
LOCUS       DAORAQ010000330         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6043_length_2452_cov_58.849395, whole genome shotgun sequence.
ACCESSION   DAORAQ010000330 DAORAQ010000000
VERSION     DAORAQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_2452_cov_58.849395"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PKM72_14890"
     CDS             <1..1286
                     /locus_tag="PKM72_14890"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNP62131.1"
                     /translation="AGGIAGIIKIFNEAILTIPKMATGLAIPLKGALNKAGLSTVSLS
                     NLGFVLDPSLLMVGFGAIIGLRAGLSLLLGAILAWGIIGPWVLTQGWIPAESLAPDGY
                     WFGPMVEWLLWPGVTLMVMASLTSFACSWGSGLTGGILSRKAPASLVTPNDPHVIPRQ
                     WFVIGLCVSLVFGVLTQITLFNISIGIAILAVLLSFVLAIVAGRVSGETGITPIGAMG
                     KVTQLTFGFLIPGNVTTNLMAANVTGGAAGQCADLLHDLKTGLLLGASPRFQALAQIF
                     GVLTGSLVGSAVYLVLIPDPQSMLLTIEWPAPAVATWKAVAEVFQLGSEAIPQGSLLA
                     MGIAGLLGVGMVVLDQALPRFISRWIPSASTMGLAFVIPAWNSLSLFLGALLGTFLMR
                     YAKTWAERFLMALAAGLVAGESLAGVASVLVKILF"
     gene            complement(1430..1654)
                     /locus_tag="PKM72_14895"
     CDS             complement(1430..1654)
                     /locus_tag="PKM72_14895"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNP62132.1"
                     /translation="MNEHQQTGGVKMKRKRQLHDGIAGALITAGVALGYYVNPLWLLV
                     PGILGVTLLQSGFTGFCPVYFILDRTCPEE"
     gene            1978..>2452
                     /locus_tag="PKM72_14900"
     CDS             1978..>2452
                     /locus_tag="PKM72_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62133.1"
                     /translation="MPFGYYHQLNAKAKRIYRASDKVSDFQLPNVSVVRPVVRKIFEA
                     LQAESHLRTQKWTQRLLNRLTSQLHIRPIRFELLDIRPSNPRMELYGLYYPMEGRRIP
                     RIQVWMRTAKRHQVVAFRTFLRTLLHELCHHLDYDCLGLKDSFHTKGFYGREASLA"
BASE COUNT          498 a          606 c          622 g          726 t
ORIGIN      
        1 cggctggtgg gatcgccggg atcataaaaa ttttcaacga agctattctg accattccca
       61 agatggccac gggcctggcc attccactta agggagcgct caacaaggcg gggttatcca
      121 ctgtgtccct ttccaatctt gggttcgttc tcgatccctc gctcctgatg gtgggctttg
      181 gagccattat cgggcttcgc gccggactct ctctactcct tggagcgatc ctcgcctggg
      241 gcatcattgg tccttgggtc ttaacgcagg gctggattcc tgcggaaagt ctggcgcccg
      301 acggctattg gttcggaccc atggtggaat ggttgctttg gccgggggtt accttgatgg
      361 tcatggcctc actcacgtct tttgcctgct cttgggggtc agggctgacg ggcggcatac
      421 tttctcgaaa agctccggca tcacttgtaa ccccgaatga tccccatgtc attccacgtc
      481 aatggtttgt gatcggactg tgtgtctctt tggtgtttgg ggtgttgact cagatcacgt
      541 tattcaatat ttcgataggt attgcgatcc ttgcggtcct actctctttt gtcttggcca
      601 ttgtggcggg acgagtttca ggggaaaccg ggattacgcc aatcggtgcc atggggaaag
      661 tcacacagct cacgtttggt ttcctcattc ctggcaatgt cacgaccaat ttgatggcag
      721 ccaatgtcac aggtggagcg gctggccagt gcgcggatct ccttcatgat ttaaagaccg
      781 gtctcctcct cggtgcgtcg ccgcgatttc aagccctggc gcaaattttt ggagtgttaa
      841 ccggctctct ggtgggtagt gcggtgtatc tggtgctcat tcctgatcct caatccatgc
      901 tgttaaccat tgaatggccg gctcctgctg ttgccacatg gaaggctgtc gcggaggtct
      961 ttcaactcgg tagtgaggct attcctcagg gaagtctgtt ggccatgggc attgcaggtc
     1021 tgctaggcgt ggggatggtt gttctcgacc aggctctccc acgattcatc agccgatgga
     1081 tccccagcgc ctcgacgatg ggactggcgt ttgtcatccc ggcctggaat tctttgtcac
     1141 tttttctcgg tgctcttctt ggaacctttt tgatgaggta tgcgaaaacc tgggcggaac
     1201 gatttttaat ggcgctggcc gccgggcttg tcgctggtga aagcctcgca ggtgtggcga
     1261 gtgttcttgt taaaatattg ttttgaactg attcaaaaaa atggccacag aggtttttga
     1321 aggtcgttgc caccttgctg tggtttcata aattccaagc cggtttcccc ttttcctcta
     1381 aaaaccactc gtcatatctg accatccttc gccgatatct gacctgcttc tattcctcgg
     1441 gacaggttct gtcgagaatg aaatagactg ggcaaaatcc ggtgaacccg ctttgcagca
     1501 gtgtgactcc aaggattccc ggaactaaca accacaaagg atttacgtag taccccagtg
     1561 cgacgcctgc agtaatgagc gcaccggcga ttccgtcatg gagttggcgt tttcgcttca
     1621 tcttcactcc tcctgtttgt tgatgttcat tcattctctc cctggattcc ttcttgccac
     1681 acaggatttt tcctgttttc ccgtggctac caaactgccc attttttgta tgtatgtttc
     1741 ttggaatgta ggaacctatg ggaggagtct aactccaggt acttttttga aaaatacgta
     1801 tttaaatcta ccttattttg cttgttttta aattgatcaa ttactgggca atacattgaa
     1861 aataaagcat aatttaatgt tttacctcag tatatctccc gtcttttctg ccaggatcaa
     1921 ggggtgctac tcttggggtg ctcgatgaaa ttcattaaac agaatggaga ccaagacatg
     1981 ccattcggct attaccacca gcttaacgcc aaggcgaagc ggatctatcg ggcaagcgac
     2041 aaggtctcgg attttcagtt gcccaatgtt tctgtcgttc ggcctgtcgt gcgaaaaata
     2101 tttgaggctt tacaggccga gagtcatttg cggacccaaa aatggactca acgccttctg
     2161 aatagattaa cgagccaact tcatattcgg cccattcggt ttgaattgtt ggatatccga
     2221 ccttccaatc cccggatgga actgtatggt ttgtattacc ccatggaagg acggcggatt
     2281 ccgagaatcc aagtatggat gcgcacggcc aaacggcatc aagtcgtcgc ttttcgcacg
     2341 tttctgcgca ccttgcttca tgagctttgt catcatttgg attatgattg tttaggcctg
     2401 aaagactctt ttcataccaa gggcttttat ggtcgtgaag ccagtttagc ct
//
LOCUS       DAORAQ010000331         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6155_length_2420_cov_66.556448, whole genome shotgun sequence.
ACCESSION   DAORAQ010000331 DAORAQ010000000
VERSION     DAORAQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6155_length_2420_cov_66.556448"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(912..1772)
                     /locus_tag="PKM72_14905"
     CDS             complement(912..1772)
                     /locus_tag="PKM72_14905"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HNP62134.1"
                     /translation="MSQMMTRSRGVGVIGVILGLSVQLVGGAELTGQANRQQMRDVAT
                     VAIEPRKNHAAVFLEETFPIVDRRFVQVADDKQRVSSGKEALYQPPQRGAPGGRVGGG
                     TRGPSADLPLMWALVPDHVGLSAEVQPRLVWYLSKKTTYPLELTIIEATGVNPIIEKG
                     ISSPTEPGIQIIRLADYDLRLEKGKTYQWSVALISDPEHRSRDLIVGGMIQVGDVPVS
                     LGENMRNANPVEATRLWGQAGFWYDAIGVISTQIQSNPSDPEMHNVRASLLEEVDLDM
                     PAQVDRQHGL"
     gene            2227..>2420
                     /locus_tag="PKM72_14910"
     CDS             2227..>2420
                     /locus_tag="PKM72_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62135.1"
                     /translation="MLKCGFLKRLDYGPLVFILILGSGGLGFTNSSGEEISPQTLMED
                     GSRHYQQGQLEQATRSWTQAA"
BASE COUNT          656 a          662 c          554 g          548 t
ORIGIN      
        1 atcgccccgc acagaagggc ccatgccatt aaaaacttgt ggaatctatt cagatggagg
       61 agaaacatgc cagtgaaaag gaaagagggc ccatttgaaa gctaaacacg aaaacgcctc
      121 agggttggta tggttttggc cacacccctt tggagtcaat atcggctcga caaaatgaga
      181 cttaattcgt ataagaaaaa cggagcatca ccattcctac caagtaccag aagacataca
      241 tggctgaaac gaagtgatga atctggtgag aggattaaga aaaaaattcc caatcagaat
      301 gcgatgacga ggaacggaat ctacaggtga gcctgtcagc gcccaggaag agcggagtac
      361 aagggatttt ggtatctcgc agaagcaacc gttcgatgca ccggaaagca gcttaccgga
      421 agaacggtgt cgatgaagac gttcaagtcc tctaaaaaat gggctgttga atatttcaag
      481 tttttgcccc gcttattcac aattgaattg gtggtttagg ctcacaggga gtaaataatg
      541 gactattcaa aaatgcgcgt ccagtcctgt cctgaggctt cgcgaaggga aggccgcagg
      601 cacttggacg cgcggagggt atggaaaagt acgtgagcac ggccaagagg cgaaaacacc
      661 gctggcgaca tttctcaaca ttccaaaaaa tgaaaacctg tccccgccac aacggcaggg
      721 acaggttcca taactgcggg tacgatgaag ggggaaggga gccggctccc ctagaacgga
      781 agaagccggc tcaataactc acgggcatca cggactgaag gaaatttctg gtcctgtcaa
      841 cacccgccga gacaagacct tcgtgggctt gcccatacaa acctccaatt cccactgtca
      901 aaaagccgca gtcataatcc atgctggcga tccacctgcg ccggcatgtc caaatccacc
      961 tcttcgagca aggacgctcg cacattatgc atctcgggat ctgaaggatt agactgaatc
     1021 tgggtggaaa tcacgcctat ggcgtcatac caaaaccccg cttgccccca taacctcgtc
     1081 gcttcaacgg gattggcgtt cctcatattc tcacccagag agaccgggac atctcctacc
     1141 tggatcatcc ctccgacaat cagatctctt gatcggtgtt cgggatcgga gatcagagcc
     1201 acagaccatt gataggtttt tcctttttcc agcctcaggt catagtctgc taaccggatg
     1261 atctgaatcc cgggctcagt cggtgaggaa atccctttct caattatcgg attcactccc
     1321 gtcgcctcta tgatggtcaa ttccaaaggg taggtcgtct tctttgatag ataccagacc
     1381 aatcgtggtt gcacctcggc agacaatccc acgtggtcag gaaccaaggc ccacattaat
     1441 ggaagatcag ccgaaggacc tctggttcct ccaccgaccc gtcctcctgg agcccctcgt
     1501 tgtgggggct ggtacaatgc ctccttacca gatgaaaccc gctgcttatc atctgcaacc
     1561 tgtacaaacc gccggtcgac aataggaaat gtctcctcca gaaaaaccgc cgcgtgattt
     1621 ttccttggtt caatggctac agtcgcaaca tccctcattt gttggcgatt tgcctgacca
     1681 gttagttccg ctcctcccac taattgaaca ctaagaccaa ggatcacgcc tatgacaccc
     1741 acacccctgc ttcgcgtcat catctggctc atgatggtcc tcctctcttc gcccggaccg
     1801 tctttccgga acatttacac gtgagacatt ttcagatcta ctcacgctca ttaataatta
     1861 cataaagcaa gcttcgttcc ataagccaaa ttttcctcaa atggcgacaa tcgtttcgaa
     1921 cccaatgcta ttgcaaaaga tttcatggca aacctctcac acctggactt tctccttcaa
     1981 tgcggcaaac acctcacctg tacacatgat gtattgtgtg tccactttga gaacaatttc
     2041 aagcgcagat ggatcaccct tccaagccag cgacagaaga cgtgcatggt aaaaatcccc
     2101 gtcctccacc cgatccactg gaaccccaaa accctcccta tcacaatccc tcgctttgac
     2161 cttattgaag ggagttccta gaatagattc cgtaaaagga tcgtttcgaa agaagggact
     2221 gcaacgttgc tcaaatgcgg gttcctcaaa cgtcttgatt atggtcctct ggttttcatt
     2281 ctcatcctgg ggtcaggtgg attagggttc accaactcca gtggagagga aatatcccct
     2341 caaaccctga tggaggatgg aagtcggcat taccagcaag gacagcttga acaggccacc
     2401 cggtcctgga ctcaagccgc
//
LOCUS       DAORAQ010000332         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6209_length_2404_cov_58.669647, whole genome shotgun sequence.
ACCESSION   DAORAQ010000332 DAORAQ010000000
VERSION     DAORAQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6209_length_2404_cov_58.669647"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKM72_14915"
     CDS             complement(<1..734)
                     /locus_tag="PKM72_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62136.1"
                     /translation="MRIWPGRSYPLGATWDGQGVNFALFSENATGVDLCLFDDELQDR
                     ETHLIPIEERTDQVWHVYLPEVRPGQLYGYRVHGPYDPGAGHRFNPAKLLIDPYAKSL
                     TGVVRWSDSMFGYRLGDPAGDLSIDDRNNAANIPKGVVVDQAFSWGGDQLLNTPWEQT
                     VIYEVHVKGMTMRHPDVPESLRGTYAGLASPPIIEYLQSLGITAVELLPVQHFVNDLY
                     LKEKGLTNYWGYNSIGYFAPDIRYSA"
     gene            936..>2404
                     /gene="treZ"
                     /locus_tag="PKM72_14920"
     CDS             936..>2404
                     /gene="treZ"
                     /locus_tag="PKM72_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNP62137.1"
                     /translation="MEKLKAVRWQLELGASCIDASAVEFRVWAPKAQSVAVHIMGSAE
                     EPTPLSQEPFGYWKGSVQDISPGTRYQYVLNHTIERPDPASRSQPDGVHGPSEIIDPL
                     AFSWTDPSWRGLPLEQYIIYELHTGTFTKDGTFDAIINRLPYLRDQIGITAIELLPVA
                     QCPGSRNWGYDGTFLFAPQHNYGGPDGLKRLVNACHAAGLAVIMDVVYNHLGPEGNYL
                     GDFGPYFTDTYKTPWGSALNYDGPESDAVREFMISNAVYWIQEYHIDALRLDAIHGIF
                     DFSAKHLLQDIGEAVHAAGQRLNRQVQVIAESDLNDVRIISPISRGGHGLDGQWSDDF
                     HHALHAVLTKERKGYYEDFGQLKDLVKAIREGVVYSGQYSPHRRRKHGNSFKPCSPTQ
                     LVVCAQNHDQIGNRAQGDRLSTMVSFEALKVANATVLLSPYLPLLFMGEEYGETAPFL
                     YFIDHGDPALIEAVRQGRTREFAAFGWTDIPDPYAQETF"
BASE COUNT          623 a          639 c          592 g          550 t
ORIGIN      
        1 aaagccgaat accggatgtc cggggcaaaa tacccaatgg agttgtaccc ccaataattg
       61 gtgagaccct tttccttcag ataaagatca ttcacaaaat gttggaccgg caacaattcc
      121 acagcggtaa ttccgagcga ttgcaaatat tcaataatcg gcggagacgc caaacccgca
      181 taggttcccc gcaatgattc aggaacgtcc ggatggcgca tcgtcatgcc cttgacgtgg
      241 acttcataga tcacagtctg ctcccaggga gtattcagaa gttggtcgcc tccccagcta
      301 aaggcctggt cgacaactac ccctttcgga atgtttgccg cattgttgcg atcatcgatg
      361 gagagatcac ctgccggatc tccaagacgg tagccaaaca tcgaatcgga ccatcgcacg
      421 acccccgtca aggacttcgc ataaggatcg atgagaagct tggcaggatt aaaccggtgc
      481 ccagccccag gatcataagg cccatgcact cggtatccat aaagttgacc gggacgcacc
      541 tcaggtaaat atacatgcca gacttgatcc gttcgttcct caataggaat cagatgggtt
      601 tcccgatcct gaagttcatc gtcaaacaga caaagatcca caccagtcgc attttcggag
      661 aacagcgcaa aattgactcc ctgcccgtcc catgtggcac caagcgggta tgatcgacct
      721 ggccaaattc tcatagattc tgctctcctg gaaatgtgtt cctgattatg acaaattgcg
      781 gaggacgata atacagtata tcttaagatg cagcaaaggg aataagccgc tgccggggtt
      841 ccctggtctt cgcctatttg ttaagctttc catcagcagc aggcatttcc cccacaatta
      901 aaattatcga aaagaccagc agggaggaaa cacgtttgga aaaattaaag gccgtacgat
      961 ggcaattaga attgggggca tcgtgcatcg acgcctctgc tgtagagttc agggtatggg
     1021 cacccaaggc tcagtcggtg gccgtccaca tcatgggaag tgctgaggaa ccaactcctc
     1081 tcagccagga accctttgga tattggaaag gctccgtgca agacatttct ccaggcaccc
     1141 gctatcaata tgtattaaat cacaccattg agcgtcctga tccggcttcc cggtcgcaac
     1201 cggatggggt gcatggccca tcagaaatta tcgatcccct tgcattttcc tggacagatc
     1261 cgtcctggcg tggcctgcct ctggagcaat acattatcta cgagcttcat accggcacct
     1321 tcacaaagga cgggaccttt gacgccatca tcaatcgtct gccctatttg cgtgaccaga
     1381 ttggtatcac cgccattgaa cttttgccgg tagcccaatg ccctgggtcc agaaactggg
     1441 gatacgacgg aacctttctc tttgcacccc agcacaacta tggtggaccc gacggcctca
     1501 agcgattggt caatgcctgt catgcagccg gtcttgcggt gatcatggac gttgtctata
     1561 accacctggg accagaggga aattaccttg gtgattttgg cccatacttt accgatacct
     1621 ataaaacccc atgggggagc gccctcaact acgatggacc cgaaagcgac gctgtcagag
     1681 agttcatgat cagcaatgcg gtctattgga ttcaagagta tcacattgat gcgttacgcc
     1741 tggatgcgat tcacggcatc ttcgatttca gcgccaagca tcttcttcag gatattgggg
     1801 aagcggtcca tgcggcggga caacggctca atcggcaggt acaagtgatt gccgaaagcg
     1861 acctgaatga tgtccgcatc atttcaccca tctcacgagg ggggcatggg ctggacgggc
     1921 aatggagtga tgactttcat catgccctgc atgccgtact cacaaaagag cgcaagggct
     1981 actatgaaga ctttgggcaa ctaaaagatt tggttaaggc catacgagaa ggcgtggtct
     2041 attcaggaca gtattcgccg catcgacgac gaaaacatgg caattccttc aagccatgct
     2101 caccaacgca acttgtggtg tgtgctcaaa atcatgatca aatcgggaat cgcgcacaag
     2161 gagatcgcct cagtaccatg gtgagctttg aagccctcaa agtcgccaat gccacggtgc
     2221 tgctttcacc ttatcttcct ctcttattta tgggagaaga atacggcgaa accgcgccat
     2281 ttttatattt tatcgaccac ggggatcctg cgttaattga agccgtccga caggggagaa
     2341 caagagaatt cgcggcgttc ggatggacgg acattcccga cccctatgct caagagacct
     2401 tcga
//
LOCUS       DAORAQ010000333         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6254_length_2393_cov_64.171086, whole genome shotgun sequence.
ACCESSION   DAORAQ010000333 DAORAQ010000000
VERSION     DAORAQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6254_length_2393_cov_64.171086"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..671
                     /locus_tag="PKM72_14925"
     CDS             258..671
                     /locus_tag="PKM72_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNP62138.1"
                     /translation="MGGSNPYIEKSETAVAKKPFKLTFITPEKPIEIEVDPEKFPYGE
                     TGLPGSILDIALGNGIDVEHACGGVCACSTCHIIVKQGLDTCNEATDDENDQLDEAPG
                     LTLQSRLGCQCVPNGEKDLIIEIPEWNKNLVKESH"
     gene            887..>2393
                     /locus_tag="PKM72_14930"
     CDS             887..>2393
                     /locus_tag="PKM72_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNP62139.1"
                     /translation="MAARNALFGSGPLSYSSVKRIFRAADIGKGLLVLLILSLQYFPG
                     VGQEDQLLAQVRPGFDPTGRFGQPPPLEKKTEPLPPKPAPDITLPPVETIPELEGAPP
                     QIRVMVRKIQVKGSTVFSEEELAKVTAPFENRELSTNDLEELRRALTLLYVNKGYVNS
                     GAVIPDQTLQDGVVTIQIIEGKLTDIQIEGTKYFLPFYFEDRIALSSGPPLNINPLKE
                     KLQLLLQDPRTERLNTELKPGLKPGEGVLHVQVEEASPFRAWVEFNNFQSPTVGESRG
                     LGNIAVQNPFGLGDAFHFTYGQSKGLQPLIEANYIIPLTARDTTLELNFRFNDFNVVT
                     KPFDDLDIRTKSQIYKIALRQPIYRTLNDEIALSLVGEHLENQSFLGGTGFSFQRGAT
                     DNGKAIVSALRFGQEWIHRESNQVLAIRSRFSVGLNVLDATNNKQTSDDPDGEFFVWL
                     GQAQYVRRVDPLGIEFLGGLTLQIANDSLFALEQFAVGGRYSVRGYWENYLV"
BASE COUNT          663 a          596 c          554 g          580 t
ORIGIN      
        1 catatattgg agagccatca gggcataatc tgcttttttc gaaagcttca gcataattaa
       61 gtattaatat tggtaaattt caattagcca taataccgac ttatatgatc ggacttaaca
      121 tagcattgac ctctttggtc agtcaagact aaaatggtcg gagatccatt tagtcggatt
      181 agtaaggacg aaaaatgacc caacgtgaaa ttttttgaaa tttaattttt aacatattga
      241 tttataagga gaatttaatg ggaggcagta atccatatat tgagaaaagt gaaacggcgg
      301 ttgcaaaaaa accatttaaa cttaccttta tcacaccaga aaagcctatt gaaattgagg
      361 ttgacccgga gaagttccct tatggagaga caggattacc tgggagcatc ttagatattg
      421 cgctgggaaa tggcatcgat gtggaacatg catgcggcgg agtctgtgcc tgctcaacct
      481 gtcatatcat tgtgaaacaa gggctcgaca cctgtaacga ggccactgat gacgaaaatg
      541 accaattaga tgaagctcca gggctcaccc tgcaatcaag actcggatgc cagtgcgtcc
      601 cgaatgggga aaaagatctc atcatcgaaa tcccggaatg gaacaaaaat ctcgtaaaag
      661 aaagtcacta accaaaccgg attgcttaca cgaaacagag gttgatggag taggttttct
      721 tccactcact ctatcccatt agaacttctt tgaataaggg gggccaccct caatagcgac
      781 gagaaccagc cttcatcgat catcagatgg ttccctagta attattgact ctttcctgta
      841 taacaacatc cctagagcag acaacctctt ctactgaatg ccattcatgg cggcgaggaa
      901 tgcgttgttt ggttcaggtc ccctctccta ttcatccgtc aaacggattt tccgggcggc
      961 agacatcggg aagggacttc tcgtcctcct catcctatcc ctacaatatt ttccgggagt
     1021 cggccaagaa gatcaactct tggcacaggt ccggcccggg tttgatccta cgggacgatt
     1081 cggtcaaccc ccgccgctcg aaaaaaaaac ggaaccccta cccccaaaac ctgctcccga
     1141 tattacactg cctccggtgg aaaccatacc tgaattggaa ggggcccctc cccagattcg
     1201 ggtcatggtt cggaaaattc aagttaaggg aagtaccgtc ttttcagagg aagagctagc
     1261 caaggtcaca gccccattcg aaaaccggga gttgtccacc aatgatctgg aagaactgcg
     1321 ccgggcttta accctgctct atgtcaataa aggatatgtg aactccggag cagtgatccc
     1381 ggatcagacc ctacaggatg gagtcgtcac gattcagata attgagggaa aactgacgga
     1441 tatccaaatc gaaggcacca aatattttct accgttttat tttgaagacc ggattgccct
     1501 gagcagcgga ccaccgctca atatcaaccc gttgaaggaa aaactccagc tccttcttca
     1561 agacccgcgc acggaacggt tgaacacgga gttaaaaccc ggcctgaaac ccggcgaagg
     1621 ggtccttcat gtgcaggtag aggaagcttc accattcagg gcatgggttg aattcaacaa
     1681 ctttcagtca cctacggttg gcgagtcgcg tggtctcgga aatatcgctg tgcagaaccc
     1741 gttcgggttg ggcgatgcct ttcactttac ctacggccaa tccaaaggat tgcagcctct
     1801 cattgaagcc aactacatca tcccgctcac cgcacgggat actaccctcg aactgaattt
     1861 ccgattcaat gactttaacg tcgtcacaaa accattcgat gatctggata tcaggaccaa
     1921 atcccaaatt tataaaatcg ctttacgtca gcccatctat cgcacactca atgacgaaat
     1981 tgccctttct ctcgtcggag aacatttgga gaatcaaagt ttcctcggtg gaacggggtt
     2041 ttcctttcag cgcggcgcga ccgataacgg caaggctatc gtgtcagcac tccggttcgg
     2101 gcaggaatgg attcaccgcg agtccaatca ggtcctcgcg attcgatccc gatttagtgt
     2161 gggattgaac gtactggatg ccaccaacaa taaacaaacc tcggatgatc cggacgggga
     2221 attttttgtc tggttaggcc aggcccaata tgttcgacgc gtcgatcctc tcggcatcga
     2281 atttctcggc ggcctgacct tacagattgc caacgacagc ttattcgcac tggaacagtt
     2341 tgccgtcggc ggccggtaca gcgtccgggg gtactgggaa aattatctgg tgc
//
LOCUS       DAORAQ010000334         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6263_length_2391_cov_73.746147, whole genome shotgun sequence.
ACCESSION   DAORAQ010000334 DAORAQ010000000
VERSION     DAORAQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6263_length_2391_cov_73.746147"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..345)
                     /gene="rpsU"
                     /locus_tag="PKM72_14935"
     CDS             complement(151..345)
                     /gene="rpsU"
                     /locus_tag="PKM72_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNP62140.1"
                     /translation="MEVRVINNNVEKALKVAKKKLAAEGLFRELKRRRFYEKPSVKKK
                     GKEREAARRRQKWLSKNRMF"
     gene            complement(525..1916)
                     /locus_tag="PKM72_14940"
     CDS             complement(525..1916)
                     /locus_tag="PKM72_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNP62141.1"
                     /translation="MYDFIINFSYLVASVLFIFGLKGLTHPRTAVRGNLLGACGMLLA
                     VIVTLTDRHIVSYEYIILGMVVGGAIGAVLALRIEMTSMPEMVALLNGFGGAASIFVA
                     GAALIEGSSLTDSPILQLTIATAASGLIGAVTFWGSLVAFGKLKGLISENAVLFSGQQ
                     VINSILAVLVLGLSIGVVLNPESFSLYWMLVGVASILGILLVLPIGGADMPVVVALLN
                     SYSGLAAAATGFVLSNNVLIITGSLVGASGIILTHIMCKAMNRSLTNVLFGVMGPSGE
                     GGQTADEVYGGKVKSASPEEVALLFDAARRVAIIPGYGMAVSQAQHPVASLAALLESR
                     GTTVEYGIHPVAGRMPGHMNVLMAEADVPYDSLKDMDDINQTIDQVDVVLVIGANDVV
                     NPLARTDPSSPIAGMPIIDVDKAKIVVVIKRSLSPGFAGIPNPLFALDNSLMLFGDGK
                     KMILDIIAALKDS"
     gene            complement(1923..2186)
                     /locus_tag="PKM72_14945"
     CDS             complement(1923..2186)
                     /locus_tag="PKM72_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HNP62142.1"
                     /translation="MSFTVFVLAIFVGFEVITKIPPTLHTPLMSGSNAISGITLVGAI
                     VSTGTELWVTTVLGFLSVVLASINVIGGFMVTNRMLAMFKKKN"
BASE COUNT          719 a          611 c          469 g          592 t
ORIGIN      
        1 aaggattggg gtaggaagtg gccttatggc cttcttaacc agactgagga agacaacatt
       61 gtcatcctca ttcatatttt ccttaaatta gacttggaga gaaaaattga ggagaaattg
      121 cgatgttgca ggcaaatagt tactggaagg ctaaaacatt ctattctttg aaagccattt
      181 ttggcgacga cgtgcagctt cacgctcttt accctttttc ttaacgctgg gtttttcata
      241 aaatctgcgc cgtttcaatt cacgaaataa accctctgcc gctaactttt tcttagcaac
      301 tttcaatgct ttttcaacgt tgttattgat aactcgtact tccacgtttc cctcaaccta
      361 aagattaaat caacatttta cagttcaatg gcctgctacc gagtctggca ctttatcacg
      421 agttggaatc ctatgcaaga cgaaaataat tcgaatattc agacatttta ttttaaaatc
      481 aacagactat gcgttttcaa tagctttgcc taaaaagact gcactcagga gtcttttaag
      541 gcggcaataa tatccaatat catttttttg ccgtccccaa ataacattaa agaattatcc
      601 agggcgaata atggattagg aattccagca aaaccagggc ttaagctgcg cttaatcacc
      661 acaacaatct ttgccttatc aacatcaatg ataggcattc ccgcaatggg actgcttgga
      721 tcagtcctgg ctaatgggtt caccacatcg ttggcaccaa taaccaggac tacatcaacc
      781 tgatcgatgg tttggttgat gtcatccata tcctttaggg aatcataagg aacatcggct
      841 tctgccatca gcacattcat atgacctggc attcttcccg ccacagggtg aatgccatat
      901 tccacggttg ttccgcggct ctcaaggagc gcagcaaggc ttgccaccgg atgctgtgct
      961 tgagatacag ccattccgta acctggaata atggccactc gccgcgccgc atcaaacaac
     1021 aaggcaactt cctcaggaga tgccgactta acttttccgc catacacttc atctgcagtt
     1081 tggccgccct cgcccgaagg ccccatgacc ccaaacaaaa cattggtcaa cgagcggttc
     1141 atggccttgc acataatatg ggtcaggatg atcccggacg caccgactaa tgaaccggta
     1201 ataatgagga cattattgga aagcacaaaa cctgttgcgg cggcagctaa acccgaataa
     1261 gaattcaata atgcgaccac cacaggcata tccgcaccac caataggaag cacaagcaaa
     1321 attcctagaa tagatgccac cccaaccaac atccagtaca gcgagaagct ttcaggattt
     1381 aggaccaccc cgatacttaa tcccagcacc aatacagcca atattgaatt aataacctgt
     1441 tgaccggaaa acaataccgc gttttcggag atcagtccct tcagctttcc aaaggctacc
     1501 aaactacccc aaaacgtcac ggccccgatt aaacctgatg cagctgtggc aatggttaat
     1561 tgaagaatgg gtgaatctgt taaggaagaa ccttcaatca atgcggcccc agccacaaaa
     1621 atcgaggcag cgccaccaaa cccatttaaa agggcgacca tctcgggcat ggaggtcatc
     1681 tctatgcgca gcgctaacac cgctccaata gcgccaccca ccaccattcc caaaataatg
     1741 tactcataac tgacaatgtg tcgatcagta agggtcacta tcacggccag cagcattcca
     1801 caggcaccca gaagattccc gcgaactgcc gttcgaggat gggtaagccc tttcaaacca
     1861 aaaataaaca ggacggacgc aacgaggtaa gaaaaattta tgataaaatc gtacatatag
     1921 ttttaattct tctttttgaa catggccaac attcggttcg tcaccataaa cccaccaatc
     1981 acattgatgc tcgccaagac cacagacaga aaacccaaaa ctgtagtaac ccacagttcg
     2041 gttcctgtag aaacaatggc tcccactaag gtgatacctg agatggcatt agaacctgac
     2101 attaaaggag tgtgaagagt gggcgggatc ttcgtgatga cctcgaagcc cacgaagatc
     2161 gcgagaacaa atactgtaaa cgacatgaga aacatttcca tagcaattcc ctcgttctta
     2221 cgccgattga gctggaagcc ccaataactc tcgaatgcgc ggctgaacaa cttccccatt
     2281 tcgcgtcatc atggtttctt ttgtaatttc gtcgttcata tcaagttgaa tctccccttt
     2341 tttcactaaa tggaggagaa aggtcgccac atttttggca tacatttgac t
//
LOCUS       DAORAQ010000335         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6298_length_2381_cov_58.755374, whole genome shotgun sequence.
ACCESSION   DAORAQ010000335 DAORAQ010000000
VERSION     DAORAQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_2381_cov_58.755374"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1970
                     /locus_tag="PKM72_14950"
     CDS             <1..1970
                     /locus_tag="PKM72_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNP62143.1"
                     /translation="VVCSSSDQSLPLQRFKILSTPADPAEAVLQGLSRLSHHPGRHIV
                     HGSTVATNAILERKGARTALITTKGFRDVLLIGRQNRPDLYDLFPAIPPTLVPREWSF
                     EILERVDCAGNILTPLQEQDLVSILEMFRREAIQSVGVSFLFSFVNPAHEQWVTARLR
                     DEGFFVTGSSEILPEFREYERTSTTVVNAYVSPVLDAYLGRLEREIKPTEFHIVQSNG
                     GRISVAQARGHGVRSILSGPAGGVVGARYLAGLAGFTRILTYDMGGTSTDVALVNETI
                     HVTTEAKVGGNPIRVPVIDIHTVGAGGGSLATVDAGGNLRVGPQSAGAQPGPVCYGRG
                     GTIPTVTDAHVVLGRLPTDGFLGGRMRLNVEAAQQAFDELSRQMVLTPRSGLDIRQTL
                     ALGMIQIANVHMERALRVISVERGEDPRETVLVAFGGAGGLHACDLARALGIPHVMVS
                     PMAATLSALGMLAADVQLDYVQTVMLPGSTPMEILEQSTQPLVDQGQEDLHREGVDPD
                     QWVLSRTVDVRYVGQSFDLAVPLTSKFHEEFHRIHHARYGYSQTETPIEIVNVRVRAV
                     GHVTPPTIPTRPLGSADPTVAKWEDRPVVLPQGIQPVPHYWGTRLCPGHEINGPAILV
                     LDDTTVYLGPTDHAAIDTYSNILIKVGATDG"
     gene            1963..>2381
                     /locus_tag="PKM72_14955"
     CDS             1963..>2381
                     /locus_tag="PKM72_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNP62144.1"
                     /translation="MDDPIRTEMIKHRLVSVAEEMGARLQRAAFSPNIKERCDFSCAV
                     FDASGNLVAQAAHIPVHLGAMPLSVQACLESLTLAPGDVAMVNDPYRGGTHLPDLTVV
                     SPIFMTNTSPPTLLGLVANRAHHADIGGMSAGSMPLS"
BASE COUNT          463 a          616 c          708 g          594 t
ORIGIN      
        1 ttgtggtctg ttcctcctcg gatcaatctc ttcctcttca gcgatttaaa attctctcga
       61 ccccggccga tcctgcggag gcggttctgc aagggttatc ccgtctttct caccatcccg
      121 gtcgccatat tgtgcatggt tccaccgtgg ccactaatgc cattctggaa cgcaaagggg
      181 cgcgaacggc tctcatcacg acaaaaggct ttcgcgatgt gctactcatc ggacggcaaa
      241 atcgtcctga tttgtatgac ctgttccctg ccattcctcc gacgttagtc ccgcgtgaat
      301 ggtccttcga aatcttggag cgggtggatt gtgcagggaa catcctgacg cctcttcagg
      361 aacaagatct tgtctcaatt cttgaaatgt tccggcgtga ggcgattcag tctgtgggcg
      421 tgtcgtttct gttttccttt gtgaatcctg ctcatgagca atgggtgacg gcgcgcttgc
      481 gtgacgaagg attctttgtg acgggttcct ctgaaatcct gccggaattt cgggaatatg
      541 aacgaaccag tacgacggtg gtgaatgcct atgtgtctcc cgtgctcgat gcctatttgg
      601 gccgtttaga acgggagata aaaccgaccg agtttcatat tgtgcaatcg aacgggggac
      661 gtatttccgt cgcccaagcg cgagggcatg gggtccgctc cattctgtcc ggtccggccg
      721 ggggtgtggt gggtgcgcga tacctggcag ggttggcggg cttcaccagg attctgacct
      781 atgatatggg cgggacgtca acagatgtgg cgttggttaa tgagacgatt cacgtcacga
      841 cagaagccaa agttgggggg aatcccattc gtgtgcctgt catcgatatc catacagtcg
      901 gtgccggagg tggctccctg gctaccgtcg atgcgggcgg taatcttcgt gtgggacctc
      961 aaagtgccgg agcgcaaccg ggtccggtgt gttatgggcg gggcgggacg attccgacgg
     1021 tcaccgatgc ccatgtggtc ctgggtcgat tgccgactga cgggttttta ggtggccgga
     1081 tgagattgaa tgtcgaagcg gcgcaacagg cgttcgatga actgagccgg cagatggttc
     1141 tgacacctcg ttccggtttg gatatccggc agaccttggc cttgggcatg atccaaattg
     1201 ccaatgtcca tatggagcgc gcgttgcgtg tcatctcggt ggagcgggga gaagatccga
     1261 gggaaaccgt cttggtggca tttggtggtg caggcggatt acatgcgtgt gatctggcca
     1321 gggcgttggg tatcccacac gtgatggtgt cacccatggc cgccacgctc tcggctctgg
     1381 gcatgttggc tgcggatgtg caattggatt acgtccagac ggtgatgttg ccgggaagca
     1441 cgccgatgga gattcttgaa cagtcgactc aaccgttggt tgatcagggg caagaagact
     1501 tacatcggga aggcgtggat cctgaccaat gggttctctc tcgaaccgtc gatgttcgat
     1561 atgtcggtca atccttcgac ttagcggtgc cgctgacttc caaatttcac gaggaatttc
     1621 atcggatcca tcacgcacgc tatggctata gccagacaga gacgcctatc gaaattgtaa
     1681 atgttcgagt ccgggcagtc ggccatgtga cgccgccgac cattcccacc aggccgctag
     1741 ggtcagccga tccgactgta gccaagtggg aagatcgtcc ggtagttttg cctcaaggga
     1801 ttcaaccggt cccgcattat tggggaaccc gcttgtgtcc gggacatgaa atcaatggac
     1861 cggccattct ggttctggat gataccacgg tctatctcgg acccacggat cacgccgcga
     1921 ttgataccta ttcgaatatt cttataaagg tgggagccac cgatggatga tcccattcgc
     1981 acggaaatga ttaagcaccg cttggtgtct gtggctgaag aaatgggcgc tcgcctgcaa
     2041 agggctgcct tctcgcccaa catcaaagaa cgctgtgatt tttcctgtgc ggtgtttgat
     2101 gcatcaggaa atctggtcgc gcaggcggcg catatcccgg ttcatcttgg agccatgccg
     2161 ctgtctgtgc aagcctgtct tgagtccttg accttggcgc ctggtgatgt ggccatggtc
     2221 aacgatccct atcgcggggg cacacatctt cccgatctca cagtggtctc gccaattttt
     2281 atgacaaaca cctctcctcc tactctatta gggttggtcg ccaatcgtgc gcaccatgcc
     2341 gatattggcg ggatgtcggc agggtcgatg ccgttgtcac a
//
LOCUS       DAORAQ010000336         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6303_length_2380_cov_65.397849, whole genome shotgun sequence.
ACCESSION   DAORAQ010000336 DAORAQ010000000
VERSION     DAORAQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6303_length_2380_cov_65.397849"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="lipB"
                     /locus_tag="PKM72_14960"
     CDS             complement(<1..668)
                     /gene="lipB"
                     /locus_tag="PKM72_14960"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNP62145.1"
                     /translation="MRMTLSDSRQPGMILTFPHLPYAEAWELQQSLATQRLEDHRPDT
                     LVLTEHEPVITLGRTTKPEHWEPHWPELQDMGIHLHQTGRGGSVTYHGPGQLIGYPIL
                     RLRNFCPGPKIYVQQLEEVLIRTLGEWGIAGCRHESFRGVWVDSEAGHLEKIASIGVR
                     ISRGVTMHGFALNVNVDLKPFPLLAPCGIEGCTMASMAKNLGRSIDSHLVRTQIIHHF
                     GEVFG"
     gene            complement(675..1079)
                     /locus_tag="PKM72_14965"
     CDS             complement(675..1079)
                     /locus_tag="PKM72_14965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNP62146.1"
                     /translation="MEVRIYYEDTDCGGVVYYANYLKYFERARTHYLDNHGLSVAALI
                     KEGTEFRVIHAELSYRSAATYGDTLVVETTVAADRRTSLTFSHVITDRTSKRLVVEGS
                     ATLVAVNTNGKIKRLPPPILEALGLPLVSFSS"
     gene            complement(1098..2360)
                     /locus_tag="PKM72_14970"
     CDS             complement(1098..2360)
                     /locus_tag="PKM72_14970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HNP62147.1"
                     /translation="MSAHIPTKALLLAIQSDPTSAIAIVRHLNPDMVCFFLHESHKEV
                     VESQIPPALSRMPQRWDWIFTPSPESFSACQKALAQGLGPLLTIWKVTPGELVIDITS
                     ATVGMASAMVLTGFPFSTKVLLFPGHPFPSVDQAIEAITKVLSQSEASANPWDEEATR
                     LRHEACYHFNHGSYDAAVKGFHTLEHRISGGLKPFYRALADVAQAYGLWDQLLYRTAW
                     EKLKGGIKALELASVWGGPPGMDRLLHLLKGNLTFLERIVLDSKDIKPGVSLDLLAWA
                     KRRGDRVRDLEAATHVLLRALEAFAQSRLFTQYHLKSWDVSLEQLPEDLRDTCRRQFL
                     NEIDGKYRLPLQAQFQALAALGDPMGERFVTDWSKMKSLFDAADHALLGYGFEPIKPE
                     RFHQLYELVIKLSGVAPTDLPEFPTLNV"
BASE COUNT          592 a          590 c          638 g          560 t
ORIGIN      
        1 ccaaaaacct ccccaaagtg gtgaatgatt tgtgtccgaa ccagatgcga atcaattgat
       61 ctcccaaggt tctttgccat ggaggccatg gtacagcctt caatgccaca tggcgctaag
      121 aggggaaacg gtttgagatc gacgttcacg tttaaggcaa atccatgcat ggttacccca
      181 cgggaaattc gtacaccgat ggacgcaatt ttttctaaat gacctgcctc actgtcgacc
      241 cacaccccgc gaaacgattc atgacgacat ccggcaatac cccattcccc taaggtgcga
      301 attaggacct cctccaattg ctggacatat atttttggac caggacagaa atttcgaagt
      361 cgtagaattg gatatccgat caactgtccg gggccatgat aggtcaccga tccgcctcgc
      421 ccggtctgat gaaggtggat tcccatgtcc tgcagttcag gccaatgagg ctcccaatgc
      481 tccggtttgg ttgttcgccc caaggtgatg acgggttcgt gttcggtcaa gaccaatgtg
      541 tcgggtcgat ggtcctcaag acgttgggtt gccaaagatt gttgaagctc ccaggcttct
      601 gcatagggca ggtggggaaa cgtcagaatc attcccggtt gtcgagaatc ggagagagtc
      661 atacgcatgt gttctcagga agaaaaggat actaacggga ggcccaaagc ttccaggata
      721 ggaggtggga ggcgtttgat tttgccattg gtattgactg ccacaagtgt tgccgatcct
      781 tccacgacca atcttttgct ggtgcgatct gtgatgacat gggaaaacgt gagagacgtt
      841 cgtcgatcgg ccgccactgt cgtctcgacc actagggtat ctccataggt ggcggccgat
      901 cgataggaaa gttcagcgtg tatcacgcga aattcggttc cttcttttat caatgcggca
      961 accgacaggc catggttatc caagtaatgc gtgcgggctc gttcaaagta tttcagatag
     1021 ttggcgtaat agacgactcc cccacagtcc gtatcttcat aatagatccg tacttccatt
     1081 tcggcctgtt gcctccgcta cacatttaga gttggaaatt ccggaaggtc ggtgggagcc
     1141 acgccactta attttatcac cagctcatag agttggtgaa agcgttcagg tttaatgggt
     1201 tcaaatccgt agcccaacag cgcatgatca gcggcatcaa ataaggactt catttttgac
     1261 caatcagtga caaagcgttc ccccatggga tctccgagcg cagccaacgc ctgaaattgc
     1321 gcctgcagag gaaggcgata tttcccatca atttcgttga gaaattgtct gcgacaggtg
     1381 tctcgcaggt cctcagggag ttgttcgaga ctcacatccc aacttttcag gtgatactgg
     1441 gtaaacaatc tggattgagc aaaggcctcc aaggctcgaa gcaggacatg agtggcggct
     1501 tcaagatccc gaacccggtc tcctcggcgt ttagcccatg ctaataagtc gagagacact
     1561 cccggtttaa tgtcttttga atccagcaca attcgttcaa gaaacgtgag atttcctttg
     1621 agtaagtgaa ggagacgatc cattccagga gggccgcccc acacagaggc cagttccaag
     1681 gctttgatcc cgccctttaa tttttcccag gcagtacgat agagcagttg atcccagagg
     1741 ccataggcct gtgcgacatc ggccaaggcc cgatagaagg gtttcagccc gccgctgatg
     1801 cgatgttcca gtgtgtgaaa ccctttaacg gccgcatcat atgagccatg gttgaaatgg
     1861 taacaagctt cgtgtcgtag gcgcgtcgcc tcttcgtccc aaggattggc agaggcttca
     1921 gattgcgaca gtaccttggt gatcgcctca atggcctggt caacagatgg aaatggatgg
     1981 ccgggaaaca acaacacttt ggtggaaaaa ggaaatcctg tcagcaccat ggcggaagcc
     2041 ataccaactg ttgcgctggt tatatcaatc accagttcgc ccggcgtcac cttccagatg
     2101 gtcaacagag ggccaagacc ctgggctaga gccttctggc aggctgaaaa actttccgga
     2161 gaaggagtga agatccagtc ccaacgttgg ggcatgcggg acagggcggg agggatttgg
     2221 gactccacga cctctttatg ggactcgtgc agaaaaaaac agaccatgtc aggattcaag
     2281 tgccggacaa tagcgatggc ggacgtagga tcggattgaa tggcaagtag gagtgctttg
     2341 gtggggatat gggcactcac aatgggtcga ctatatcatc
//
LOCUS       DAORAQ010000337         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6309_length_2379_cov_66.743546, whole genome shotgun sequence.
ACCESSION   DAORAQ010000337 DAORAQ010000000
VERSION     DAORAQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6309_length_2379_cov_66.743546"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..917
                     /locus_tag="PKM72_14975"
     CDS             105..917
                     /locus_tag="PKM72_14975"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNP62148.1"
                     /translation="MNPLPWNILTLPLGYPIAGILLVLFVGLWLLHLRVGNASLADVG
                     FCLGFGFVVFVCGIEGEGSPWRRALVVSMGGAYACRLSWHLWKNRIWRKSEDPRYKTI
                     RAVLGKWESIGIFWYFVLQVPVCLFFGFLLCWIMGHPESAVRSWDFLGLGIFLLAFFG
                     EALADIQLERFRSDPTKQGKVLQTGLWRYSRHPNYFFEILQWCAYVPLAVGLPGAWMA
                     IVWPLLMMSSLLWVTGVPLAEAQAMNSRGEGYRLYQRTTNKLFPWLPRRNKS"
     gene            935..1354
                     /locus_tag="PKM72_14980"
     CDS             935..1354
                     /locus_tag="PKM72_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62149.1"
                     /translation="MLWDSQAGFRHCWKNPRGFLVLAGLLVLMTLAPGCAFVRGEVGE
                     PFPEDGLQTIEKGITNRQEVAQQFGAPDDIVQANGHEIFHYRRFDSKLGWLLFFSRLN
                     IGSDHLWVFFNPQGIVDDVVFGNRTKNVEFQIWPFGE"
     gene            1359..1778
                     /locus_tag="PKM72_14985"
     CDS             1359..1778
                     /locus_tag="PKM72_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62150.1"
                     /translation="MDGVFGKAGILGVLLLFSLSGCDFRRVVVNDPLVADSLEVLVPG
                     KSTMQDIATALGAPDEIEEGASGMVFRYRYGDSKTMRVNFGWLLRVLLPFAPSMNLGR
                     GEGVTYILHVALRPDLTFDHSVIQPPPDTPHFWFWPF"
     gene            complement(1892..>2379)
                     /locus_tag="PKM72_14990"
     CDS             complement(1892..>2379)
                     /locus_tag="PKM72_14990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNP62151.1"
                     /translation="GVSKTDYAMIVKVFGMIALMAGGLIGGLVVYRMGIVPSLFILGF
                     LQMISTAGFVILAYTGNHLPTLTAVIAIETISSGLGQTAFVAFMASLTNKRFTATQYA
                     LLTSFMGIPRVFAGSTTGFLATWLGWEGFFLLCTLIALPGLFLIPYLRRLEENNPSLN
                     R"
BASE COUNT          523 a          504 c          689 g          663 t
ORIGIN      
        1 cggaagagca aaccgttcgg agtcctttgg ccttagccgc gaattgagtc cctttccaaa
       61 gattctcatg gtcgtggtgt tgatcgtgtg ttttctgatt taaggtgaat ccgcttccct
      121 ggaatatcct cactctgccg cttgggtatc cgattgctgg tatcctcctg gtgctcttcg
      181 ttggcttgtg gcttttacat ttgcgagtgg gcaatgcctc attagcggat gtggggtttt
      241 gtttgggatt tggctttgtc gtttttgttt gcggaataga gggcgagggc agtccttggc
      301 gacgcgctct tgttgtgagc atggggggtg catatgcctg ccggttgagc tggcacctgt
      361 ggaagaatcg catttggaga aagtcggagg accctcggta taaaactatt cgggcagtgc
      421 tgggcaaatg ggagtcgatc ggaatctttt ggtacttcgt gttgcaagtt cctgtttgtc
      481 tgttctttgg atttctcctg tgctggatta tgggccatcc cgaatcggcg gtaagaagct
      541 gggattttct gggcctcggg atatttttgc tggccttctt tggagaggct cttgcggata
      601 ttcaacttga acggttccgc tcagatccga cgaaacaggg caaggtctta cagacagggt
      661 tgtggcggta ttcccgccat ccgaattatt tttttgaaat cctacagtgg tgtgcctatg
      721 ttccgttggc agttggactg ccaggggcat ggatggctat cgtgtggcct ttgctcatga
      781 tgtcgtcact cttatgggtg acaggagtgc ctttggccga ggctcaggct atgaacagca
      841 ggggggaagg gtatcgcctc tatcaacgaa ccacgaacaa attgtttccc tggctgccca
      901 gacgaaacaa gtcataaaaa agaggaggag aactatgttg tgggattctc aagccggctt
      961 tagacattgt tggaaaaatc cacgaggatt tctggttttg gccggtcttt tggttttgat
     1021 gaccctggca cctggctgtg catttgtccg gggagaagtg ggtgagcctt ttcccgagga
     1081 tgggcttcag acgattgaga aaggcatcac gaatcgccaa gaagtggccc agcaatttgg
     1141 cgcccctgat gacattgtcc aggcaaatgg ccatgagatt tttcactacc gccgctttga
     1201 cagcaagttg gggtggttgc tgtttttttc tcgcctcaat attggcagtg atcatctctg
     1261 ggtatttttt aatcctcaag ggatcgtgga tgacgtggtg tttggcaata ggaccaagaa
     1321 tgtggaattt caaatctggc cgtttgggga gtagggcaat ggatggggtc tttggaaaag
     1381 ctggaattct tggagtcctg ttgcttttct ccctgtcggg ctgcgacttt cgtcgtgtgg
     1441 tggtgaatga tccattggtg gccgattctc tcgaagtgtt ggtgccagga aaaagcacga
     1501 tgcaggatat agccacggcc ttaggagctc ctgatgagat tgaggaagga gccagcggaa
     1561 tggtctttcg ctaccgatat ggagatagca agaccatgcg ggtaaatttt ggttggcttc
     1621 ttcgtgtatt gttgcccttc gcgccatcca tgaatttggg gagaggtgaa ggggtcacct
     1681 atattttaca tgtggcgttg cgaccggatt tgacctttga tcattcggtc attcagcccc
     1741 cacccgatac cccccatttc tggttttggc cgttttaact gacactgggc gaatggtgcc
     1801 catgaggagt cttttaccct cttttggata ttagggaatc ttgatgtttg tgattatgag
     1861 aattttatct tggcgagttc aacagggcct attacctatt aagggatggg ttgttttctt
     1921 ccaagcgccg gagataggga atgaggaata atccgggcag cgcaattaag gtacagagaa
     1981 gaaaaaaccc ttcccatccc aaccaggtgg ccagaaatcc ggtggtggaa ccggcaaaga
     2041 cgcgaggaat ccccatgaaa ctggtcaaca gggcatattg tgtggcggta aaacgcttgt
     2101 tggtgaggct ggccataaag gccacgaacg cagtttgtcc cagtccactg gaaatggttt
     2161 caatggcaat gacggcagta agagtaggga ggtgattgcc ggtataggcg agaatgacaa
     2221 aacccgctgt tgaaatcatt tggaggaatc ccagaataaa aagagaaggc acaatcccca
     2281 tgcgatagac cacgagaccc ccaatcaatc cgcctgccat taatgcaatc atgccaaaaa
     2341 ccttgacgat catcgcatag tcggttttcg agacgccga
//
LOCUS       DAORAQ010000338         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6335_length_2372_cov_64.690980, whole genome shotgun sequence.
ACCESSION   DAORAQ010000338 DAORAQ010000000
VERSION     DAORAQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6335_length_2372_cov_64.690980"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..646)
                     /locus_tag="PKM72_14995"
     CDS             complement(47..646)
                     /locus_tag="PKM72_14995"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNP62152.1"
                     /translation="MRLLATIQKTGVIFLLFWVGAVWAGFEEGEEAYLQENFSAALSE
                     WRPLAEEGHAEAQNMLGYMYRYGQGVTQDFEQARLWYRRAADLGNARAQNNLGAMYRQ
                     GLGMPQDYQEAFRWFLRAAEQGNGGAQNHVGLMYYKGEGVPKDLVQAYKWAYLAAQQG
                     LDPSIQALDLLSQEMTTAQINKATDPARKWNPKGEEVAL"
     gene            complement(736..>2372)
                     /locus_tag="PKM72_15000"
     CDS             complement(736..>2372)
                     /locus_tag="PKM72_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNP62153.1"
                     /translation="GRVTRRQHLRIAYIPQQADFDSDSTVAEEIERAALASGLHAHDC
                     VARVQETLGRMGFGQGDQIVGPLSGGWKKRLAIACGFVQSPDVMLVDEPTNHLDLEGM
                     RWLEQVMSQAPWPWVMVSHDRWFLGHATNKVAEINSRYPDGIFLVSGGYEEFLVKREE
                     FLNSQTQQAQALAGKVRREEEWLRRGPKARSTKAKARIDSAHALQAELAETQVRLRQE
                     ETSIDFVGSGRRTKQLMVVHQLRKAFAKRTIIQKLDWVISPGTATGVLGHNGSGKSTL
                     LKLLAKELEPDQGSVTHADKLQVVYFDQNRDQLDPKMTLRRTLSDSGHTVMYRGQTVH
                     LAGWAKRFQFQPEQLDLPIELLSGGEQARAVIARLMLQSADVLIVDEPTNDLDIPTRE
                     VLEESLKEFPGALILVTHDRYMMEEVCTEFVGLDGQGGYGQFADYGQWESWLRRQRSG
                     KDQSSTQTKGTRVATERPKAKKLSFRDQQEYDTIEKRVLDAEAVLELCRTETENPKIV
                     SNHLKLQEAYENLKSAEQEVERLYTRWAELEAKQQA"
BASE COUNT          528 a          711 c          543 g          590 t
ORIGIN      
        1 aagggatgac aaaactcttc tcaacacatg gacgtcagac cttcacctaa agagcgacct
       61 cttcaccttt tggattccat ttccgtgcgg gatcagttgc cttattgatc tgtgcagtgg
      121 tcatctcctg tgagagtagg tccaaggctt ggattgatgg atccaggccc tgctgggcag
      181 ccagatacgc ccacttatag gcttgcacca ggtcctttgg gactccttct cccttgtaat
      241 acatgagacc cacatgattt tgcgcaccac cgtttccctg ttcggccgcc cgtagaaacc
      301 atcgaaaggc ctcttgataa tcctgcggca tccccaaacc ctggcggtac atcgcgccaa
      361 gattgttctg agctctggca ttgccgagat ccgcagcccg acggtaccac agtcgagcct
      421 gctcaaaatc ctgggtgact ccctggccat accggtacat atagcccaac atgttctgtg
      481 cttcggcatg tccctcttct gctaatggtc gccattcaga cagagcggcg gaaaaattct
      541 cctgcaggta ggcctcctcc ccttcctcaa aaccggccca taccgctccc acccaaaaga
      601 gtaaaaaaat cacgcccgtt ttctggatag tggccagaag tctcatggtg tcatttcttt
      661 ctttccctca ccgtgcggtg gactcagtaa acagcatgca tcaaccctga cagacgctca
      721 tatcttcatt gtactctatg cctgttgctt cgcttcaagt tccgcccacc gggtatacag
      781 gcgttccacc tcctgttcag cggacttgag attttcataa gcttcttgga gcttgagatg
      841 attggaaacg attttgggat tttctgtttc cgtgcggcac aattcgagga cagcctcggc
      901 atcaagaacc cgtttttcta tcgtgtcata ctcttgctga tcacgaaatg agagtttttt
      961 ggcttttggc cgttccgtcg cgacacgggt acccttggtt tgagtgcttg actggtcttt
     1021 gcccgatcgt tgacgtcgaa gccatgattc ccattgtccg taatccgcaa attgtccgta
     1081 ccctccctgc ccgtctaaac cgacaaattc cgtgcagact tcctccatca tataccggtc
     1141 atgggtaacc aggattaatg ccccgggaaa ttcctttaaa ctctcttcca ggacttctcg
     1201 cgtcggaata tccaaatcgt tcgtcggttc atccacaatc aacacatctg cggactgaag
     1261 cattaatcgg gcaatgaccg ccctggcctg ctcaccgcct gagagtaact caatcgggag
     1321 atccagttgc tcaggctgaa attgaaagcg tttagcccat ccagccagat gcacggtctg
     1381 cccccggtac atgacggtgt gcccggaatc cgacaacgtc cgtctcaagg tcatcttcgg
     1441 atccaattga tcacgatttt gatcaaaata caccacctgc aatttatccg catgcgtcac
     1501 gcttccctga tcaggctcca attccttggc gagtaatttc aagagggtgg acttcccgga
     1561 gccattatgg cctaagaccc cggtagcggt tcccggtgag atcacccagt ccaatttttg
     1621 aataatcgtc cgcttggcaa atgccttccg aagctgatgg acgaccatca attgtttggt
     1681 gcgccgacct gagccgacaa aatcaatgga ggtttcttcc tgtcgcaacc gcacttgggt
     1741 ttcagccaac tccgcctgca acgcatgcgc agaatcgatg cgagccttcg ctttagtgga
     1801 tcgagccttg ggacctcgac gaagccactc ttcctccctg cggacttttc cggccaaggc
     1861 ctgagcttgt tgagtttggc tgttcaggaa ttcttcccgc ttaaccaaaa actcctcata
     1921 gcctcccgaa accagaaaga tgccatcagg atagcgggaa ttaatttctg ccaccttatt
     1981 ggttgcatgg ccgagaaacc atcgatcatg gctcaccatg acccacggcc atggagcctg
     2041 ggacatcacc tgctcaagcc atcgcatccc ttccagatcg agatgattgg tcggctcatc
     2101 caccagcatg acatccggcg actgaacaaa cccacaggca atggcaagac gttttttcca
     2161 tccgcctgaa agaggaccca caatctgatc tccctgacca aaccccatgc gccctagtgt
     2221 ctcctgcacc cgagccacgc aatcatgcgc atggaggccg gaggccaacg cggcccgttc
     2281 gatttcctcc gcgactgtgg agtcggaatc gaaatccgcc tgttggggaa tataggcaat
     2341 gcgaagatgc tgccgtcgag tgactcttcc ct
//
LOCUS       DAORAQ010000339         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6346_length_2369_cov_61.636128, whole genome shotgun sequence.
ACCESSION   DAORAQ010000339 DAORAQ010000000
VERSION     DAORAQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6346_length_2369_cov_61.636128"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(510..1526)
                     /locus_tag="PKM72_15005"
     CDS             complement(510..1526)
                     /locus_tag="PKM72_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HNP62154.1"
                     /translation="MEKMKAVEVRQAKGPLQLVEREVPKPGTGQVLIKVQACGICHSD
                     VFTKDGLWPGLEYPRIPGHEIAGIVEEMGSGVEGWKQGQRVGVGWHGGHCGRCEPCRL
                     GDFVLCQRGLVPGISYDGGYAEYMVAPVEALARIPEDLSNLEAAPLLCAGITTFNALR
                     HSGARAGDVVAILGIGGLGHLAVQFASKMGFKTVAIARGKDKEALAKKLGARHYIDSR
                     AQNVSETLKGFGGAKVILATVTSGKAMSATIGGLAIDGKLIMVGASEEPVEVPIVQFI
                     MGRHSVQGWPSGTSSDSQDTLAFSVMTGIKPMIEEYPLERAAEAYERMMSGEARFRVV
                     LQVS"
     gene            1856..2215
                     /locus_tag="PKM72_15010"
     CDS             1856..2215
                     /locus_tag="PKM72_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNP62155.1"
                     /translation="MARFVIAGSKGTDDPTMATLPFIASQAAHEQGHEVVLWLQAEAV
                     VLAKKDGVDGVQGVGLPALKVLANTILSQGIPLWVCSACAIARQINESDLEVGAVMKG
                     MDDYVKAVAERERSLSF"
BASE COUNT          606 a          631 c          558 g          574 t
ORIGIN      
        1 atttcggatg acgaagggat taaaaaagat atgccggcct ggtgcgaaac aaccggacac
       61 gccatggtgg gtatggaaga agaaggggcc gcagacaaaa aagtctacaa agcctatgtc
      121 aaaaaggcct gagagtctgc ccttgtagaa tatctctttg ccgggtctca aacttgatca
      181 actaccgagt aagtagacgc tgggggaaga tccatccatc gaaatttcca caacgaatac
      241 cctagtcaga acctcagttt ttttggaatg tcccccgcct tccctcctac agtcattccc
      301 tgaggtgaga aaatatacat gccagggtaa cacgcatcag ttggaaaatg ccttgttcag
      361 aatatggaaa gtcgagcggt cgtgggagga acttgtctcc ttatacacgg attaacttgg
      421 ctcttttcat gcgcactgct tctttattat gcacattccc gtttttgccg aaggaaaagc
      481 attggaggag tgaccagtga gtgagctctt caactaacct gtaatactac tctgaacctt
      541 gcttcaccac tcatcatacg ctcataggct tcagccgcgc gttcaagagg atactcctct
      601 atcattggct ttatacctgt catcacacta aaggccaacg tatcctgcga atccgacgac
      661 gtaccggacg gccagccctg aactgaatgt cgccccataa taaactgcac gatgggtact
      721 tccaccggtt cttcagaggc gccaaccata atcaattttc cgtcgatggc cagtcccccg
      781 atagtcgcac tcatggcctt tccactcgtg acggttgcga ggattacctt cgctcctcca
      841 aaacccttaa gcgtttccga tacattttgg gccctgctgt cgatataatg ccgcgccccc
      901 aatttcttcg ccaatgcctc tttatctttt cctctggcaa tggcgaccgt cttgaagccc
      961 atcttgctcg caaattgtac ggccaaatgt ccaagaccgc cgatgccaag aatagcaacc
     1021 acgtcaccag cgcgcgcacc gctatggcga agcgcattaa atgtcgtaat gcccgcacaa
     1081 agcagaggag ccgcttcaag gttggagaga tcttctggaa tgcgcgctaa agcttcgacg
     1141 ggagcgacca tgtattcggc atagcctccg tcataactaa tacccggaac caagccgcgt
     1201 tggcatagaa caaaatcccc taagcggcat ggttcacagc ggccgcaatg tcctccatgc
     1261 catcccacgc caacccgttg gccctgtttc catccttcca cccccgaacc catctcctca
     1321 acaattccgg caatttcatg tccaggaata cgaggatact caagacccgg ccatagaccg
     1381 tccttcgtaa acacatcgct gtgacagatc ccgcaggcct ggacctttat aagaacctgc
     1441 cctgtgccag gcttaggcac ctcacgctcc acgagttgca aaggcccctt tgcttgtcgg
     1501 acttccacgg ctttcatctt ttccatggat gttctccttt tcattcacta tcaaatttct
     1561 catggcctgg acccgatcga accacaagcc tcaggcaggt accgaaaaat gtcacgccag
     1621 ctatcatgac agatcagaaa actctaagaa agatggatat aggcggaaac gggggagcca
     1681 acattattca cccgccccat ctaaaaccag ggttctacag aggtgtagcc ttaaattcat
     1741 cactgatacc atcaaattag gggttcccgt cttaatcctg atccagcttt cctgtgatat
     1801 gagatgaggg tgtcggagaa gcatgaaaga aaaaaggtaa taaaaggaga aaactatggc
     1861 acgctttgtc attgccggaa gtaaggggac ggatgatcca acaatggcca cccttccgtt
     1921 tatcgcctca caggcggctc acgaacaagg gcatgaggtc gttttgtggc ttcaggccga
     1981 ggccgtcgtc ttagcaaaaa aagatggggt agatggtgta cagggagtcg ggcttccggc
     2041 attgaaggtg ttagccaata ccattctgtc tcagggtatt cctctgtggg tctgttcggc
     2101 atgtgcgatc gcccgacaga ttaacgaatc tgatctcgag gtgggtgcgg taatgaaggg
     2161 catggatgat tatgttaaag ctgtggcgga aagagaacgc agtctttcgt tttaaattcc
     2221 ttccccgaag gatccagaat gctggaagtg aggttggggt tactctggac gaatcatctg
     2281 ttccggacga atgtaggtat tgaattcttc ttcagtcaca aatccgctgg cgatagcttc
     2341 ttctcgcagg gtgcgtccat tttttaagg
//
LOCUS       DAORAQ010000340         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6390_length_2356_cov_64.219035, whole genome shotgun sequence.
ACCESSION   DAORAQ010000340 DAORAQ010000000
VERSION     DAORAQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6390_length_2356_cov_64.219035"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKM72_15015"
     CDS             <1..268
                     /locus_tag="PKM72_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNP62156.1"
                     /translation="VTDVLDRIKASGYTVFSDVSGKGHSGYHKGHLMFNDTSSLQMVL
                     TVVPEDKVEPILSALKPFLERHSGSLFVLDASVLRPDHFESTNS"
     gene            complement(1010..>2356)
                     /locus_tag="PKM72_15020"
     CDS             complement(1010..>2356)
                     /locus_tag="PKM72_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNP62157.1"
                     /translation="GTMNTTGAFQYHATTLGTDSVLSRIVHLMREAQGSRAPIQKLAD
                     RVSGIFVPVVLSLAIATFMVWFVMADTAPAIRALVASVSVLIIACPCAMGLAVPTAVM
                     VATGKGAELGVLIKGGEALQRASQVTTIVMDKTGTLTKGQPAVTEILSAPGTKYTEQE
                     ILRLVASVESSSEHPLGQAIVRHAATRKYSLFPVHAFQAMTGRGAVGIVEGRKVAVGN
                     LTLMSELDIRTSAIQQDVERFAREGKTPVYVAIDGTVEALIAIADPLKASSREVVDRL
                     HRLGYQVTMLTGDHQRTAEAVARMAGITRVVAGVLPEGKVAEITRLQSAGEVVSMVGD
                     GMNDAPALAQADIGIAMGAGSDIAIEASDVTLMRNDLRAVGSAIELARQTMKTMKQNL
                     FWAFIYNVVGIPVAAGILFPIWGIMLSPILASAAMAFSSVSVVTNSLRLRMWSPEN"
BASE COUNT          499 a          636 c          648 g          573 t
ORIGIN      
        1 tgtcaccgat gtgctggatc gaatcaaggc cagcgggtat accgtcttca gtgatgtttc
       61 aggtaagggg cacagcggat accataaggg tcatctgatg tttaatgata cgagcagttt
      121 gcaaatggtc ctgactgtgg tgcctgagga taaggtcgag cctatcctct cggcgctgaa
      181 gcccttcctc gagcggcatt cgggaagttt gtttgtgctc gatgccagtg tcttgagacc
      241 cgatcatttt gaatccacca attcttaatt ctccgggata ctctaatgtg aagcatatgg
      301 tggatatgac aacatcctcc cgccggtgca aatagaagac gacaataacg tatgtcacgc
      361 tggtaggagg tatcctggtt tcccaaagat gtgttattga aggcagcaag gtttggacga
      421 gggtagcctg tgctcgcatg tatggtattt cctgtttgcc atgaatcagt agaactgttg
      481 gtcctgctaa agctttacat aacatcgaaa ccctcactca cccattttcc tatcaaatta
      541 atagcaatct gttaaatttc ggaatgcggc acgttccctc tcctgctgat attccggccg
      601 tgttgtgaat gtctgaaacg cgagatggct gtggttgtct gcaccgggtt tcgattgttg
      661 caggggctgt tgaaaaaagc cgccagcggc gttctcgcca tttttccgtg ctcacgtact
      721 ggaagtacgc tctgcgcgca aaaatgactg cggccttccc ttcggcatga ctcagggcag
      781 gactggacga acccttcgaa aacctcagga catgcttttt tgaaccgccc tgaggcctct
      841 catatgcaac gtgcctgttg gtcaatagac ccttggaaat tattattcaa cacttcattg
      901 ttccatttaa gatgacaaga atataacggt cttgtcgcgg acgaagccgg gaggaaacgg
      961 gtccgtttct tcccggctgc ctgatgcccc tcaagatggc ttacgatgtt cagttctcag
     1021 gcgaccacat gcggagccgg aggctgttgg tgacgacact gacggaactg aaggccatgg
     1081 ccgcactggc cagaatgggg ctgagcataa tgccccagat gggaaaaagt atgccggccg
     1141 caaccggaat gcccacaaca ttatagataa aggcccagaa caaattttgc ttcatggtct
     1201 tcatggtctg ccgtgccaac tcgatggcgg atccgaccgc acgcagatca ttccgcatga
     1261 gcgtgacatc gctggcctcg atggcaatat cggatcccgc tcccatagcg atgccgatat
     1321 ccgcttgggc gagggcgggt gcgtcgttca ttccatcacc gaccatggac acgacttctc
     1381 ctgccgattg gagccgcgta atttcagcca cctttccttc gggtaagacg cccgccacta
     1441 cccgtgtgat tccggccatt cgtgccaccg cttctgccgt gcgttggtga tcgcccgtga
     1501 gcatcgtgac ttgatacccc agccggtgca ggcggtccac cacctcgcga gaagaggctt
     1561 tcagcgggtc ggcaatggct attaaggcct ccaccgtacc gtcgatcgcc acatacacgg
     1621 gtgtttttcc ttctctggcg aatcgctcga catcctgttg gatggcgctg gtccggatgt
     1681 ccaactcaga catgagtgtg agattcccta ctgcgacctt tcgtccttca actatgccta
     1741 cggcgcctcg cccggtcatc gcttgaaacg cgtgcaccgg aaataaggag tatttcctgg
     1801 ttgcggcatg tcgcacgatg gcttgcccaa gtggatgttc cgacgaactt tccactgagg
     1861 ccaccagacg aaggatctct tgttcggtgt atttcgtgcc gggggcggag agaatttcgg
     1921 tgacggccgg ttgtcctttg gtgagcgtac cggttttgtc catgacgatg gtcgtgacct
     1981 ggcttgcccg ttggagcgct tctccacctt taatgagcac gcccagttcg gctccttttc
     2041 cggtggccac catgacggct gtgggcaccg ccaatcccat cgcacagggg caggcgatga
     2101 tcagtactga caccgaggcg accagggcgc gtatggcggg ggcggtatcc gccatgacaa
     2161 accacaccat aaatgtggca atcgccaacg acaggactac gggaacaaaa atcccgctga
     2221 cacggtcggc cagtttttga atcggcgcgc gtgacccctg ggcctcgcgc atgaggtgta
     2281 ctatgcgaga gaggacactg tctgtgccta aggttgtggc gtgatattga aacgcgccgg
     2341 tggtattcat ggttcc
//
LOCUS       DAORAQ010000341         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6435_length_2344_cov_79.367409, whole genome shotgun sequence.
ACCESSION   DAORAQ010000341 DAORAQ010000000
VERSION     DAORAQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_2344_cov_79.367409"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKM72_15025"
     CDS             complement(<1..533)
                     /locus_tag="PKM72_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62158.1"
                     /translation="MAWFPPGWAFADSPNISDHSPREFSARLIWKTGGHIAKAQLFAK
                     KDRYRIEPAGGIRTGLGYAGVLIIRLDKQKTWYMLSQRRMILSVPLILDYLLPFSIRL
                     EGETSRMVIGESVVGDQPALLYDVEVKDRFGQLERFFEWVDPQREVLLKLLSQDRDWF
                     VEYHHVVLSSQPDYYFEA"
     gene            complement(617..1540)
                     /locus_tag="PKM72_15030"
     CDS             complement(617..1540)
                     /locus_tag="PKM72_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNP62159.1"
                     /translation="MKQKRVMEIVVIIGLLWITSSTAFGYEVIDVTDGATIVGTVTLK
                     GIPPSPKAYNLVTFPDPEYCGRISDGQGWRLLKDFVVNDHNQLQGVVIVVEGVNAGKP
                     FSLSIPKVEARDCQFLPFTTVVRDEHGIEVVNMDPVMHDIQAYETSLSQGTRVLFNSP
                     LPFNPRHQREDIHATHEHLPGKSLVHQFQLSKGRKTFVMQCGFHAYMESWGIAVDNPY
                     FNFTNERGEYVISSLPPGSYRIRAWHPSVKHEQVYEFSVEPNQTVRRDFSLESPVGRW
                     TAHTIRTPPRFSAAALGHPVLIEPLVEHQRP"
     gene            complement(1555..>2344)
                     /locus_tag="PKM72_15035"
     CDS             complement(1555..>2344)
                     /locus_tag="PKM72_15035"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNP62160.1"
                     /translation="PRPMAFNLVTIPDAVYCGRISTGTGWRIVEDFIIGPQGSLKDAV
                     VMVKGVDKGKPFSLPKVEVEAIDCDFLPFVNVLKDQDEITVINMDPVEHDIQGYETAR
                     ERGARVLFNRPLPMNPFHNVVGMFGENVHTHLPGKPMIEKIHLRKGRDVFVMQCGFHP
                     YMFSWGLVVENPYYSISKEDGAYEISDIPAGEYEVAVWHPGMKTYLEKTVRVESGKTL
                     TLDFQYESPQGRRSVHEMQENPHFGLEMLGEGVEIIPSLRRQVP"
BASE COUNT          644 a          636 c          486 g          578 t
ORIGIN      
        1 gcttcaaagt aataatctgg ttgcgaagaa agcaccacat gatgatattc cacaaaccag
       61 tcccgatcct ggcttaagag tttcaagaga acctcccgtt gaggatccac ccattcgaaa
      121 aatcgttcga gttggccaaa acgatctttg acctccacgt catagagcag ggcaggttga
      181 tcccctacca cggattcccc aataaccatt cgactcgtct ctccctccaa ccggatggaa
      241 aatggtaaca aataatccaa aattaaggga acgcttaaaa tcattcgccg ttgcgacaac
      301 atataccagg ttttttgttt atccaatcga ataatgagaa cgcccgcata gcctaaccct
      361 gttcgtattc cccctgccgg ctcaatgcga tagcgatcct ttttcgcaaa caactgggct
      421 ttggcgatat ggcctcccgt cttccatatg agtcttgcgg agaattccct gggactgtgg
      481 tcagagatgt tgggagagtc ggcgaaggcc cacccgggcg ggaaccaagc cactattcca
      541 aaaatggcac aacccaccac gcccgccaat ttcttatcac aagttcggga gaaaagaagg
      601 gaaacgtcta tccatttcat ggtctttggt gttcaaccaa aggctctatt agaacagggt
      661 ggcccagagc agcagcagaa aatcgaggcg gggtcctgat ggtatgtgcc gtccaacggc
      721 ctacgggaga ttccaaggaa aaatcccttc tcacggtttg attcggttcc acggaaaatt
      781 cataaacctg ctcatgcttc acacttggat gccatgcacg aatgcgataa cttccaggcg
      841 ggagtgatga aataacatac tctccccttt cattggtaaa gttgaaatag ggattatcca
      901 ccgcaatccc ccagctttcc atgtaggcat gaaacccgca ctgcatcaca aaggttttac
      961 gtcccttgct cagttgaaat tgatggacca aggatttccc cggcagatgc tcatgtgtcg
     1021 catgaatatc ttcacgttga tgccggggat taaagggcag aggagaatta aataacaccc
     1081 gtgtaccttg gctcaacgac gtctcatagg cttgaatatc atgcatgacc ggatccatat
     1141 tcaccacttc aatcccatgc tcatcacgaa caaccgtcgt aaatgggaga aattgacagt
     1201 cgcgagcttc caccttcgga atagaaaggg aaaacggttt cccggcattt acaccttcaa
     1261 ccacaatcac gaccccttga agttgattgt gatcattcac cacaaagtcc ttaaggagtc
     1321 gccaaccctg gccatcagaa atgcggccgc aatactctgg atcagggaac gtcaccaggt
     1381 tgtacgcttt aggagaaggc ggaatgccct tgagagtcac cgtgccgaca atggtggccc
     1441 catccgttac gtcaatcact tcataaccga aagcggtcga actggtgatc cagagcaagc
     1501 ctattatcac gacaatttcc atcactcgct tctgtttcat gctcacccct tctctcaggg
     1561 aacttgtcgt cgtaacgacg ggataatctc aacgccctct ccaagcatct ccagcccaaa
     1621 atgcggattt tcctgcattt catggacgct tcgccgtccc tggggagact catattgaaa
     1681 gtctaatgtc agcgtttttc ccgattccac ccggactgtt ttttctaaat aggttttcat
     1741 accaggatgc caaacagcca cctcatattc tcctgctgga atatcgctaa tttcgtaggc
     1801 tccatcctct ttggaaatcg aataataggg attttccacc actaatcccc aagaaaacat
     1861 atagggatga aatccacatt gcataacaaa cacatcacgg ccctttcgca aatgaatttt
     1921 ttcaatcatc ggttttccgg gaagatgggt atggacattt tccccaaaca taccaactac
     1981 attgtgaaag gggttcatgg gcaacggacg attaaacaag accctggccc ctcgttctct
     2041 tgccgtttca tatccctgga tatcatgttc taccggatcc atattaatca cggtgatttc
     2101 atcctggtcc tttaagacat tgacaaatgg caaaaaatcg caatcaatcg cctcaacctc
     2161 gacctttggc aaagaaaacg gtttgccttt gtctacccct ttcaccatca ccaccgcatc
     2221 cttaagcgat ccctgaggcc caataataaa atcctcaaca atgcgccacc cagttccggt
     2281 agaaattctt ccacaatata cggcatccgg gatcgtgacg agattaaacg ccatcggtct
     2341 tggg
//
LOCUS       DAORAQ010000342         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6491_length_2334_cov_61.196139, whole genome shotgun sequence.
ACCESSION   DAORAQ010000342 DAORAQ010000000
VERSION     DAORAQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_2334_cov_61.196139"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..944
                     /locus_tag="PKM72_15040"
     CDS             309..944
                     /locus_tag="PKM72_15040"
                     /inference="COORDINATES: protein motif:HMM:NF018712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HNP62161.1"
                     /translation="MADQWMLRGVEFANCNCSYGCGCQFNAPSTNGFCEAMGSGYIEE
                     GYFNDTRLDGLNYVMLLQWPGEIAQGNGTQQIFIDERANALQREALRKILHGEATSPG
                     ATHFFVFNSTMSNVLEPQFVPIDLSIDVDARKATVNVPGLVESTGTPISNPHTGRDHR
                     ARINLPEGFEYTVAEVGNGSTKAQAGIALNLSNSYGQFNILHMNQDGVIRA"
     gene            1058..1855
                     /locus_tag="PKM72_15045"
     CDS             1058..1855
                     /locus_tag="PKM72_15045"
                     /inference="COORDINATES: protein motif:HMM:NF021465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HNP62162.1"
                     /translation="MSPSPAPSFSSLHRRDRITILSALIGVIVLAWAYVLYLWAKMPM
                     MNADAPEAILQLHPWMPIDFVFVYLMWAVMMVGMMLPGAIPMTLLYAGMVRKAERQST
                     PMAPTGAFVGGYLALWSVFSVGATVVQWGLHETAMLSPMMKANSQVVGAALLILAGIY
                     QMTPWKAACLDHCRAPAHFISEHWRPGVCGAFRLGLHHGAFCLGCCWALMGLLFVGGV
                     MNVLWIALITVFVFFEKILPPGIWSGGLGWVAGIGLIVCGLGMLIVT"
     gene            complement(1900..>2334)
                     /locus_tag="PKM72_15050"
     CDS             complement(1900..>2334)
                     /locus_tag="PKM72_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNP62163.1"
                     /translation="YEANMDLVGVDPHLNLYDEVWPIRTYQGQYPPAKFVFADEFTGG
                     RRGMALDSIISGGCIISGSLVQNCVLSPNVRIEAHAEVRDSVIMENVKIGERSRIRRA
                     IIDKGVVIPPDTIIGHDIEHDSQRFSVTKDGVAVVTQDSPGK"
BASE COUNT          481 a          508 c          695 g          650 t
ORIGIN      
        1 ttctttttgg tttctttgcg tgcgtcctgg ctcttgccca tggtgtgctc cttgtgttgt
       61 tagtagtacg gcttttctat ggtgccgttc ggaggcgaat ggtagcgaac ctgagagaaa
      121 aagtatagat ggcgcgatgt ggaaagttgg aatttagaag taaataaaaa gcaggacaga
      181 ggttctcgcc cgagaactct cgtgattggg aaatgctcct ggtgatgatg gaaacattgg
      241 tggcccgcat ggtaaaagat gaaaagaact gagttggtat ccggccttgc ttttgaaagg
      301 agcgcacgat ggctgatcaa tggatgctgc gcggtgtcga gtttgccaat tgcaactgta
      361 gctatgggtg tggatgccag tttaatgccc cctcgacgaa cggattctgc gaggcaatgg
      421 gctcgggata tattgaagag gggtatttca atgacacgcg tttggatgga ttgaactacg
      481 tcatgctctt gcaatggccg ggggaaattg cacaaggaaa tggaacgcag cagattttca
      541 ttgatgaacg ggcgaatgct ttgcaacgcg aagccctccg gaaaatatta catggcgagg
      601 cgacgtcccc gggggctact catttttttg tattcaatag tacgatgtcc aacgtgttgg
      661 aaccgcagtt tgtcccaatt gatctttcca tagatgtgga cgccagaaag gccacggtga
      721 atgtgccggg cttggtcgag tcgacgggta cgcccatctc caatccccat accggacgag
      781 accatcgcgc caggattaat ttaccggagg gatttgaata caccgtggcc gaagtgggca
      841 atgggtcgac gaaggctcaa gccggaattg cattgaatct gtctaatagt tatgggcaat
      901 ttaatattct tcacatgaat caggatggag tgatcagggc ctgattgtgg cgggatgcgt
      961 ttatccttgt cggttacttc ttcgtcggtc atcatcgtag tttttcaact taccggtcag
     1021 ttgctgaatg ttgcaggggt tcttatagtc catttccatg agcccctctc ctgccccttc
     1081 attttcttcg ttacaccggc gtgatcgaat aacgattctc tcagccctga ttggggtgat
     1141 tgttttagcc tgggcgtatg tcctgtatct ctgggcaaag atgcccatga tgaatgctga
     1201 cgcgcctgag gccattctgc agttgcatcc gtggatgccg atagactttg tgtttgtgta
     1261 tttgatgtgg gccgtcatga tggtcgggat gatgttgccg ggtgccatcc cgatgaccct
     1321 gttgtatgca ggcatggttc gcaaagccga gcggcagagc actcccatgg ctccgacggg
     1381 ggcctttgtt ggtggctatt tggctctatg gagtgtgttt agtgtgggcg cgactgtcgt
     1441 gcagtgggga ttgcatgaaa cggccatgct ttctcccatg atgaaggcca acagtcaggt
     1501 cgtaggagcc gcgctgttga tcctcgcagg catctatcaa atgaccccct ggaaggctgc
     1561 ctgcttagat cattgtcggg cacctgctca ttttattagc gagcattggc gacccggcgt
     1621 gtgtggcgcg tttcgtttgg gacttcatca tggtgcgttt tgtctgggct gctgttgggc
     1681 cttgatgggg ttgttgtttg tgggtggagt gatgaatgtg ctctggatcg cgttgattac
     1741 ggtctttgtt ttttttgaaa agatattgcc ccctggcatc tggagtggag gcttgggatg
     1801 ggtcgccggg atcggcttga ttgtgtgcgg actgggtatg ctgattgtga cctgagggat
     1861 tgcccgatat tgggatgtgc ctgttccggt tggacttgat tactttccgg gtgaatcttg
     1921 tgtgacgacg gccacgccat ctttggtgac agaaaagcgt tgactgtcgt gctcgatatc
     1981 gtgaccgatg atggtatcgg gtggaatgac gacgccctta tcgatgatgg cccggcgaat
     2041 acgcgaccgt tccccgattt taacattttc cataatgacg gaatcgcgga cttcggcatg
     2101 agcctcaatg cgaacatttg gtgagagcac acaattttga accagacttc cggagataat
     2161 gcagcctcct gagatgatag aatccagtgc catgcctctg cgccctcccg tgaactcatc
     2221 ggcaaagaca aatttggctg gcgggtattg gccctgatac gtgcgaattg gccagacctc
     2281 atcatagagg ttgaggtggg gatcaacccc gacgagatcc atgttggctt cgta
//
LOCUS       DAORAQ010000343         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6548_length_2320_cov_71.265784, whole genome shotgun sequence.
ACCESSION   DAORAQ010000343 DAORAQ010000000
VERSION     DAORAQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6548_length_2320_cov_71.265784"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PKM72_15055"
     CDS             complement(<1..1361)
                     /locus_tag="PKM72_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62164.1"
                     /translation="MNPHIHFWKTGLSLVVMVMMTIVPPVTATYAEHKEVFPRRSFSN
                     AMFFGDSLTDIGNGPASLTFDGEPSDGEYFTSDSGIFNNIYVPISNPVNPRKDTILPG
                     TKLRFPPTSRDSRIFRLTLPPQLPLCSNQGCVEKTHHSLNWTEYFVYNGVLKQLLKHG
                     ADLRPWIIQFNDTLPSEVSIRQSVNYAFYSALSGEGCANVNLFPLPCTFEGKSLQESI
                     FTRQDVYRNNQSETNAEANSLLRETVIIPAFRTQIDMFEVDKRLRQVEINDHTLFIVY
                     TGANDLAAAFFLFVEGTITFDQFIQALTVGIPMEIAGGVERLISLGARNILVLGQFNL
                     GLTPRLLSMEDVDGPLAKHEVAAEFDQLLTLFNQSLKTQIENFESKRIAFVDIQTPIR
                     NAANTFFIGRHAGYFFSIGEQCVDETAGIIQLGKAASCFSNNVSLPIGFWNASHLSTQ
                     FNQL"
     gene            2029..>2320
                     /locus_tag="PKM72_15060"
     CDS             2029..>2320
                     /locus_tag="PKM72_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62165.1"
                     /translation="MIDEKGTVSGFIETDGTIGLLIASNFIDWNLRVNDGSHIFNLVG
                     PKSGANSTITLTGNSTIASAKDIQFDFSGDQARIEIINPSATSPENGWALVGD"
BASE COUNT          664 a          489 c          542 g          625 t
ORIGIN      
        1 agttgattga actgtgtgct caggtgagac gcattccaaa acccaatagg gagtgaaaca
       61 ttgttgctaa agcaggaagc cgccttccct agttgaatga ttccagcggt ttcgtcgaca
      121 cattgttcgc caattgaaaa aaaatagcct gcgtgcctgc caatgaaaaa tgtattggcg
      181 gcatttcgta tcggtgtttg aatatcaaca aaggcaatac gcttgctctc aaaattttca
      241 atctgtgttt tcagggattg attaaaaagt gtcaataact ggtcaaattc cgcggccacc
      301 tcatgcttgg ctagggggcc atcgacatct tccattgaaa gtaagcgagg agtgagcccc
      361 aaattaaatt gcccaagaac caaaatgttc ctggctccga gggaaatgag ccgttcaacc
      421 cctccggcaa tctccatggg aattccaacg gttaaggctt gaatgaattg gtcgaatgta
      481 atcgttccct cgacaaagag aaagaacgcg gcggcgagat catttgcacc cgtatagaca
      541 atgaatagtg tgtgatcatt gatctcaact tgccttaaac gcttatccac ttcaaacatg
      601 tcgatttgtg tacggaaggc cggaataatg acggtctcgc gcaaaagact gtttgcctct
      661 gcattcgtct cactttggtt gttacgataa acatcttgtc gggtaaagat ggactcttgg
      721 agtgacttgc cctcaaaggt gcaaggcaac gggaagagat tcacattggc acatccctca
      781 cctgagagcg cagaataaaa cgcataattt acagattgtc gaattgaaac ttcagaagga
      841 agggtatcgt tgaattgaat gatccagggg cgtaaatcag caccgtgctt caacaattgc
      901 ttcaaaactc cgttatagac aaaatattcc gtccaattga gtgaatggtg ggttttttcc
      961 acgcatcctt ggttagaaca caatggcaat tgtgggggca atgttaaacg gaatatccga
     1021 gagtctctgc tggttggtgg aaatcttaat ttggtcccag ggagaatggt atccttgcga
     1081 gggttcacgg gattcgagat aggaacgtag atattgttga aaataccgct atccgaggtg
     1141 aaatattcgc catccgacgg ttccccatca aaggtcaggc ttgctgggcc attcccaata
     1201 tcggtgagac tatccccaaa aaacatggca ttggaaaaac tgcgtcgagg aaaaacttcc
     1261 ttgtgttcgg cataggtggc agtcactggg gggacaattg tcatcattac catgacgacg
     1321 agtgaaaggc ctgtcttcca aaaatggatg tgtgggttca tgttccctcc ttctaagttt
     1381 aggtaacaag tcctgcctat caaggttatt ttggaattca gttgttcttt agcgatatgg
     1441 cgttgatgag tatagcaaag ttggaaactg atccaactca ttctcttctc ctcttgataa
     1501 gtctgaacgc cgcatcgtgg ttcttctgag ttgaatgggt tcaatccttg cacgagttat
     1561 gggaaccaca cagtcaggct gatcaacata tccgccaaga ctacagatgt tatgttgtct
     1621 atcgctaata aatgaagtgg tatatgcagt agacagcttt ccattattag accaggaata
     1681 gagatcacca gggacttacg tatatgatta atagttccta tcaagagcat ggtaaacaaa
     1741 ttcattaaat ctcccctttt gcaggagtca gttagcagaa tcgccaggtt tacttctctg
     1801 agaacgttga caatagtagt tcaccaggtt caccattccg tgttggaaca taatggcgaa
     1861 cttttcttat tttggggagg agaggggcca cgtcgatcac gttcactaag gaatataacc
     1921 atgaggaaat ttaaaaaagg ttcgcaccat tttgggagta tgattgtaat ttttttcctt
     1981 gcgcagatgc ccctacaatc cttagcgata acatacgatg tgaatcgagt gattgatgaa
     2041 aaggggactg tctcaggttt tattgagacg gatggaacaa taggcctgct catcgcatca
     2101 aacttcatag attggaatct ccgtgtaaac gacggctccc atatatttaa ccttgtgggc
     2161 ccaaaatctg gagcaaattc gacaatcacc ctaacgggca attcaacgat agcatccgcc
     2221 aaagacattc aatttgattt ttcgggagat caagcccgaa tcgaaatcat caacccatca
     2281 gccacaagcc cggagaatgg atgggcgctt gttggagact
//
LOCUS       DAORAQ010000344         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6556_length_2318_cov_64.226248, whole genome shotgun sequence.
ACCESSION   DAORAQ010000344 DAORAQ010000000
VERSION     DAORAQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6556_length_2318_cov_64.226248"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKM72_15065"
     CDS             complement(<1..887)
                     /locus_tag="PKM72_15065"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HNP62166.1"
                     /translation="MLNRRQFLFVMGALSALTALPKARSGAGTSGIQFDTAKPFSFDE
                     LKQRAKAMATLPYEEPVVRHDAVLESIDYDAFQEIVFKRERGLGEDGSVNFPAQFFHL
                     GRYFKVPVKLHALDDGRSREILYRSEYYTFGGTSLGKVFPDDLGFSGFRLQDGPGATT
                     DWMAFLGASYFRSSGELNQYGLSARGVAIDPALPTPEEFPRFTEFWLEPGTPDSKEFV
                     VYALLDGPSVTGAFRMVCIKNQQILMDIDANLYPRQDIKRLGIAPLTSMFWYAENNRH
                     QIRDWRPEIHDNDGLTMWTG"
     gene            1645..>2318
                     /locus_tag="PKM72_15070"
     CDS             1645..>2318
                     /locus_tag="PKM72_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62167.1"
                     /translation="MRKAHGKVLPLGALVFVLVMLGGVFPSMAQATGLTINFAGSGTG
                     RVEANSPGTDTLNIFSSNTLDYDQNSSVTLTAAPTGIGASQSVFSGWAGCTNTSGNEC
                     TVLMDGARSVTVTFNLVPVSPPVTPPVNLSLNFAGTGTGQVAGSGAGTAPFTIFNSQT
                     ISYDVVQPPSSVTLTATPTGLGASQSTFAGWSGAGCTGTGTCTVSMSAARTVTATFTL
                     VQRTLTV"
BASE COUNT          571 a          566 c          628 g          553 t
ORIGIN      
        1 ccggtccaca ttgtcagacc gtcgttatca tggatttctg gccgccagtc cctgatctga
       61 tggcgattat tttcggcata ccagaacatg ctggtcagcg gtgcgatccc caggcgtttg
      121 atgtcctggc gcgggtagag attggcgtcg atatccatga gaatctgctg gtttttgatg
      181 cagaccatgc ggaacgcacc ggtgacgctc gggccgtcga gcagagcata gaccacaaat
      241 tccttgctgt ccggggtgcc cggctccaac cagaattcgg tgaatcgggg aaattcctcc
      301 ggggtgggca gggctggatc gattgccacg cctcgcgccg agaggccgta ttgattgagt
      361 tccccggaac tgcggaaata agaagcaccg agaaacgcca tccaatcggt ggttgctcct
      421 ggtccatcct ggaggcgaaa gccggagaag ccaagatcat ccggaaagac cttgccgaga
      481 ctggttccgc caaaggtgta gtactcggaa cggtaaagaa tttctcggga ccggccatca
      541 tcaagagcat gaagcttaac gggcactttg aaatagcggc cgagatggaa gaactgagcc
      601 ggaaaattca cgctgccgtc ttcgcccagc ccccgctcgc gtttgaatac gatttcctga
      661 aaagcgtcgt agtcgatcga ttccaagacc gcgtcatgac ggaccacggg ctcctcatag
      721 ggcaacgtgg ccattgcctt ggcgcgttgc ttcagctcat caaatgagaa aggtttggcc
      781 gtatcgaact ggattccgga ggtcccggct ccacttcgcg ccttggggag ggcagtcaag
      841 gcactgagag cgcccattac aaagagaaat tgtcggcgat tcaacatatt tggaccatct
      901 gcgaggggaa agaaaactgt gaagacagca taggattttg ccctaaccct tgtggcgcat
      961 tgtcagccaa tcccaaaaca cgggtactca ttagagaatt accacccaca gccatatttt
     1021 ggtctccgta gagggcttcc gattctggcc ctcggctcga cgtttggtga taagaatagt
     1081 aaatggattt ggggagggtc agataaatac caaagaattc accggagggt caatcagcgc
     1141 ggccagggat cggaggggaa aacgcttgaa ataaacatgt atgcgcgaat ttttgataaa
     1201 tttgaggggg attgaaacac gctaccctgg attgtcagac gaagaagtta tcggaatggt
     1261 gttttggaga tgatcatgaa taggttgatc aatagagtgt tctcttaaag cgaatttttt
     1321 tgttccatta atatgtagaa tgatgtagac tacgatatct ttcttcataa ttaagctgag
     1381 ttatcgtgga ccaggatagt gtcatgcccc caacgtcctt tcctgacatg cctacctgcc
     1441 tgctccaggc aatttccaaa ctatttccat atattcctca ggatcatggc ttccctgatc
     1501 cgaatcacga cgccttgaaa attaattgaa aacccatata cggtggacag aaatgccttt
     1561 ttgttaacca gacagcttga agatatgtaa aagccataag cggtctagag aatattgatg
     1621 aaacgaaatg agaaaggacg cacaatgcgg aaagctcacg ggaaggtatt gcctttaggg
     1681 gctctcgttt ttgtcctggt aatgctaggg ggagtgtttc cgtccatggc tcaggccact
     1741 ggcctcacaa tcaactttgc aggatcaggc actggaaggg tagaagccaa ttctccgggg
     1801 actgatacgt tgaacatttt ttcgagtaac acactagact atgatcaaaa ttcctctgtc
     1861 acgcttaccg ccgctccgac cgggatcggg gcatcacagt cagtcttcag cggttgggca
     1921 ggttgtacaa acacatccgg aaatgagtgc accgtgctta tggatggtgc tagatcggta
     1981 accgtgacct ttaatctcgt cccggtcagt ccaccggtca cccctcccgt taatctttct
     2041 ctcaattttg cgggaacggg aacgggacag gttgctggaa gtggtgcggg gacagcgccg
     2101 ttcactatat ttaatagcca aacaatatcc tatgacgtag tgcaaccacc ttcttcggtt
     2161 accctgacgg ctactccgac cggtttaggg gcatcccagt caacctttgc tggttggagc
     2221 ggtgccgggt gtacgggcac gggcacttgt acggtgagta tgtcggccgc acggacggtg
     2281 acggccacgt ttactctcgt gcaacggacg ctgacggt
//
LOCUS       DAORAQ010000345         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6662_length_2286_cov_65.603317, whole genome shotgun sequence.
ACCESSION   DAORAQ010000345 DAORAQ010000000
VERSION     DAORAQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6662_length_2286_cov_65.603317"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2050)
                     /locus_tag="PKM72_15075"
     CDS             complement(<1..2050)
                     /locus_tag="PKM72_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNP62168.1"
                     /translation="MLIGDDKWGEWCALVLWIFGLGTFMGVGLVQAVEQDNDKKVWIV
                     TGIVQYEDFRRVPQVTVELRDQEGTIIDTQVSNEGGEFIFAPPESGIYSIQATQSDLS
                     SESTILKIENDPLLEVKLTLAHRQELTLDVVASLPPIQSRLSGESYSVSRKDIEELPR
                     GNNIALNDLLLTLPSAVDGGLKQVHIRQDHANFQFRIDGVPVPDTVSSVFTDVISPRT
                     WERADIILGGPEAQFGNKTAAVIDITTKSGTQPDSGSLQFFGGSNETLNPSFEYGGTV
                     GSEFRYYMMNSFISTNRGINPPTLGKSVFHDQSNQNQSLFRGDWQIDNQNSVTVLFLN
                     SIAKFEIPTQPGLAANPTLVGLIQGDNPGFEPVNSQNINEYQKENNQYSQLVWRQDWS
                     HDRFFSLAGFFQHSRATFTTDPFNALSYTSDPDEPFSAGDQDRWAYTGGIRFDYSHRL
                     NSNHLVKTGFQIQRTQAVNKTRLSAFLRDEAGDPVGGVISRNGDNRTIGWREEFWIQD
                     QWTPWAQWTFNLGVRFDQIQALTNDMQVSPRIGATYAMTPNHVFHAFYGRLFTPPNIE
                     SVPFLQLNTVGTTAEPENLTNTTVKPERAHYFEVGSAHALGDSLSIELTGYYKLSKNL
                     SDAGQFGTTPLLNYFAFKRGWQRGIDLAVKAKFLENLYGRGNVAWGQCRGKKLQSGFM
                     L"
BASE COUNT          650 a          556 c          508 g          572 t
ORIGIN      
        1 acaacataaa acctgattga agttttttcc ctcgacactg gccccatgcg acattcccac
       61 gcccatatag attttctaaa aacttggctt tcacagccaa gtcgattccc cgctgccaac
      121 cgcgtttaaa cgcaaaataa ttaaggagcg gggttgttcc gaattgtccg gcgtcggata
      181 aatttttact taatttataa taaccggtca attctattga caaagaatcc cccaaggcat
      241 gggcagaacc gacctcaaaa taatgagccc gttcgggttt caccgtggta ttagtcaaat
      301 tctctggctc cgccgtagtt ccaaccgtgt tcagttgaag aaaaggcaca ctttctatat
      361 ttggcggagt aaacaatctc ccgtagaagg catgaaagac atgatttgga gtcatggcgt
      421 atgtagcacc tatccgcgga ctcacttgca tatcattggt taacgcctgg atttgatcga
      481 agcgaacacc aagattaaag gtccattgag cccagggagt ccattgatcc tgaatccaga
      541 actcctcgcg ccatccaatg gtgcgattat cgccattccg agagatgact ccccctaccg
      601 gatcaccggc ttcatctctc aaaaatgccg ataggcgggt tttattcaca gcttgggtcc
      661 tttgaatctg aaatcccgtt ttcactaaat gattcgaatt taatcgatga ctgtaatcaa
      721 accgtatccc tccagtatag gcccatcggt cctgatctcc ggccgaaaaa ggttcgtcag
      781 gatctgacgt atacgataag gcattgaagg ggtcggttgt gaatgtggct cgggaatgct
      841 gaaaaaaccc ggccagactg aaaaaccgat catgactcca atcctggcgc cagacgagct
      901 gggaatattg attattctcc ttttggtatt catttatatt ttgagaatta actggctcaa
      961 aacctggatt atctccttgg atcagtccaa ctaaagtagg attggccgct aggccaggtt
     1021 gagtgggaat ctcaaatttg gcaatcgaat tcaggaacaa caccgtgaca ctgttctgat
     1081 tatcaatctg ccaatctcca cggaaaaggg attggttttg gttactttgg tcatgaaata
     1141 cgctttttcc taaagtggga ggattaattc ctctgtttgt agagataaag ctattcatca
     1201 tgtaataccg aaattccgag ccaacggttc ccccatattc aaaagaaggg ttcagtgttt
     1261 cgtttgagcc accaaagaat tgcaatgagc cagagtcggg ttgggtcccg cttttcgtcg
     1321 ttatgtcaat taccgccgcg gttttattcc cgaattgcgc ttccggccca cccaaaataa
     1381 tgtctgcgcg ctcccaggta cgtggggaaa tgacatccgt aaagacagaa gatacggtat
     1441 ccggaacggg gaccccatca atccgaaatt gaaaattagc gtgatcctga cgaatgtgca
     1501 cttgcttcag tcccccgtct acagcactag ggagggttaa aagcagatca ttaagggcaa
     1561 tattattccc tcggggtaat tcctcgatat ccttacgact caccgaatag gattctccgg
     1621 agagacgact ctgaatggga ggcaatgacg ccacaacatc aagagtgagt tcctggcgat
     1681 gtgccagggt taacttgacc tcgagcaaag gatcattttc aatcttaaga atagttgact
     1741 cgctcgataa gtcagactgg gttgcttgaa tagaataaat accagactca ggaggagcaa
     1801 aaataaactc ccccccttca ttcgacacct gagtatcaat gatggtgcct tcctgatcgc
     1861 gcaattccac cgttacctga ggaactcggc gaaaatcctc gtattgcact ataccggtta
     1921 caatccagac ttttttgtca ttgtcttgtt ccacagcttg gacaagacca acacccataa
     1981 acgtccccaa tccgaaaatc cagagaacaa gggcacacca ctccccccat ttatcatccc
     2041 cgatcaacat acagaaacct ccagcatcta ataatcaatt aggcgtatct ccccttgcgt
     2101 catcacaggc aacccccgtt aggaaagccg cgaatggatg aatcgacggt gaaagaccgt
     2161 tgtaaaaaag aaaattagga gaagaaggaa gctggaggac cgcgagggtt accgagggga
     2221 atgatgagag agggatacct ctcttctcga tcaggggata ggaaaccggc aactacagaa
     2281 caatga
//
LOCUS       DAORAQ010000346         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6749_length_2265_cov_63.713575, whole genome shotgun sequence.
ACCESSION   DAORAQ010000346 DAORAQ010000000
VERSION     DAORAQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6749_length_2265_cov_63.713575"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKM72_15080"
     CDS             <1..591
                     /locus_tag="PKM72_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62169.1"
                     /translation="SKSFVLHLDMWYAQSHPDEDFAETFAVWLTPHSHWRERYAGWPA
                     LKKLEYMDGLMKELVGVAPIVTTREQVDPIHRLHKTLREHYQGKRERYGLDYPNFYDR
                     DLRRLFSGDPDQSTNLSAENFIRKFRKEVRRKVAAWTGEYQYTIDQVLQDMMAHCRER
                     NLRLAVSEEQAKVDFTILLTVQTMNYLHGGRHRVVL"
     gene            588..1610
                     /locus_tag="PKM72_15085"
     CDS             588..1610
                     /locus_tag="PKM72_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNP62170.1"
                     /translation="MRKLRVMALMHQDLVPPDDVEHADLAEVEWKTEFDVVSTLRDLG
                     HEVMAVGVRDDLSVIDNLVTDWKPHIAFNLLEEFNGNPEFDQNVVSYLELLGVPYTGC
                     NPKGLVLTRDKGWSKKIMAYHGIRCPEFVVYPMGRVIKWAEEFPFPVIVKSISEEASL
                     GISQASIVHDEEKLKERVEFVHQSVGTSVIVERYIEGRELYVGVLGNRRLQALPVWEL
                     HLKNLPPDALPIATARVKWSTKYQKKYGIQSGEVEGLSLKLVRHIQQTAKRVFHILGL
                     NGYARMDFRLDPQEHLYILEANPNAQLAYGEDLAESAERAGISYEALIQRILNIGLSW
                     KPESQR"
     gene            complement(1814..>2265)
                     /locus_tag="PKM72_15090"
     CDS             complement(1814..>2265)
                     /locus_tag="PKM72_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNP62171.1"
                     /translation="NGSWDGRWGVNYTYGTFLAVRGLRASGSPTANSAIQRAATWVRS
                     IQNQDGGWGESCASYEKQEFVPASSTPSQTAWGLLALEAAGDRTSKNVHQGIEWLLSH
                     QNQQGGWDEELMTGTGFPNVFYLKYHLYAHYFPLLALATWQAGLKNS"
BASE COUNT          557 a          492 c          619 g          597 t
ORIGIN      
        1 agtaaaagtt ttgtgttgca tcttgatatg tggtatgccc agagtcatcc cgacgaggat
       61 tttgccgaaa cctttgcggt ctggctcacc ccgcactcac attggcgtga gcgttatgcc
      121 ggatggccgg cacttaaaaa attggagtat atggacgggc ttatgaagga gttggtgggg
      181 gttgcgccca ttgtgacgac cagggagcag gtggatccga ttcaccgtct tcacaaaacg
      241 ctccgggaac attaccaggg caagcgggaa cgatacgggc tggactatcc caacttttat
      301 gatcgggact tacggcgtct cttttccggg gatccggatc aatcgacaaa tctgtccgcg
      361 gagaatttta ttaggaagtt cagaaaagaa gtccggcgga aggttgctgc atggacggga
      421 gagtatcaat ataccattga tcaggttttg caggatatga tggcgcattg ccgagaacgg
      481 aacttacggt tagccgtttc tgaagagcag gcaaaagtgg attttaccat tttattgacg
      541 gtgcaaacga tgaactattt gcatggtgga cgccatcggg tggtgttatg agaaaacttc
      601 gtgtcatggc ccttatgcat caagatctgg tgccccccga cgatgtggaa catgccgatt
      661 tggccgaggt cgaatggaaa accgaatttg atgtggtctc cactctacgc gatcttggcc
      721 atgaagtgat ggccgtcggc gtgagggacg atttaagcgt cattgataac ctggtaacgg
      781 attggaaacc tcacatcgcc ttcaatctgt tggaagaatt caatggaaat ccggagttcg
      841 atcaaaatgt ggtgtcctat ttggagctcc tgggagtgcc ttataccggt tgtaatccca
      901 aggggcttgt gctcactcgt gataaaggct ggtcaaaaaa aattatggcc taccatggca
      961 ttcgatgccc ggagtttgtg gtctatccta tggggcgggt gatcaagtgg gcagaagagt
     1021 ttccctttcc ggtcattgtc aaatccatta gtgaggaagc atccctcggt atttctcaag
     1081 cgtctatcgt ccatgacgag gaaaaattaa aagaacgtgt tgaatttgtg catcagagtg
     1141 tgggcacgag tgtcattgtc gagcggtata ttgaaggacg ggaattatac gtgggtgtgt
     1201 tagggaatcg acgtttgcaa gccctcccgg tctgggaact tcacctgaaa aatcttcctc
     1261 ctgatgctct gccgattgcg actgccagag tgaaatggag cacgaaatat caaaaaaaat
     1321 atgggatcca atcgggtgag gtcgaggggt taagtcttaa gttggtgcgt catattcaac
     1381 aaacggccaa acgggttttt catattttgg gcttaaacgg ctatgcacga atggattttc
     1441 ggttagaccc tcaggaacat ctctatattc tggaagccaa tcccaatgcc cagctggcct
     1501 atggtgaaga tctcgccgag tcggctgaac gagccggtat ttcctatgaa gcgctgattc
     1561 aacgaattct gaatatcggt ttgagctgga aacccgaaag ccagcggtaa ggggagtcct
     1621 agaataatat gccccgagca aggtcgaagg aattgtcggg tgggactttt tgtctattcc
     1681 ctcttgctct gagtcgtagg ccgtaaaatg atttccttga gcccctgtct tgcgtttctg
     1741 cgagaaaagg acccttgtgt aaatattgcg tggttataac aaacggacta gtttgttcga
     1801 gagaaatgca tggtcatgaa ttctttaatc ctgcttgcca ggtggcaagg gccaacaagg
     1861 gaaagtagtg tgcgtacaaa tgatatttca aatagaaaac gttagggaac ccggttcctg
     1921 tcataagttc ttcgtcccac cctccctgtt gattctgatg tgaaagtagc cattcgatcc
     1981 cctgatgaac attttttgaa gttcgatcgc cagccgcttc aagtgcgagt aagccccagg
     2041 ctgtttgtga aggtgtgctt gatgcgggaa caaattcctg cttttcatag ctggcacagc
     2101 tttcccccca accaccatcc tggttttgga ttgagcgtac ccatgtggct gcacgctgga
     2161 ttgcggagtt tgcagttggg gaaccacttg cgcgaaggcc gcgtacggcg aggaatgttc
     2221 cataggtata atttacgccc cagcgtccat cccaactccc gttgg
//
LOCUS       DAORAQ010000347         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6806_length_2255_cov_62.386364, whole genome shotgun sequence.
ACCESSION   DAORAQ010000347 DAORAQ010000000
VERSION     DAORAQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_2255_cov_62.386364"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..1290)
                     /locus_tag="PKM72_15095"
     CDS             complement(352..1290)
                     /locus_tag="PKM72_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62172.1"
                     /translation="MMRRTPNRHFVNEILRAGVFGMALVLVLTGGSQNVLAVSQELNH
                     MVLVATPKGRIAIQKSGHKEPSLPTLIKPSVNPTANILRARSGSTSPLDALQTSNNPI
                     LAEECLVDFSDYDALDLLEFSLHPYAETTFAYTPWWHQACNGLMYVEVRPIWTPLSVS
                     HFHLGYENPHITTCPGSFTDWGIIQDDGTCQEIEPRDEPRSLHPHEGHRPTHIFVTDG
                     GINDKKLFKLNRIRVESTQGIQLCYKPMQEIDGPWISSGVAGSTHPGIWYCWDELPTG
                     NWDLSDWTDHITEVKIRASHGSFGIYQIDDIGIGIY"
BASE COUNT          558 a          528 c          550 g          619 t
ORIGIN      
        1 accaattttc aatgaggacg gccatgtcat tggccttgtg agttccggta tggattccaa
       61 cagtgaggat gacggggtgg gatgatcatt ttgggttcaa ccattacggt tttttgaaac
      121 atggcttccc gctgtcgatc cccaaaaccc gggaaggatt cgtggttggg cagtgctaag
      181 gcgtgatcct tggcatttgc aaggcttata tgcggagagc ttgcaagctg aagagatctt
      241 gcaaaccatt ggggttgact acgaaattat atatggatca aaccaatatg gatccgatga
      301 ttttttttgg agtggaactt aagacttaaa tatctgcctt ctccatattt tctaataaat
      361 tccaatcccg atgtcatcaa tctgatagat accaaagcta ccgtgactgg ctcgaatttt
      421 cacttcggtt atatgatccg tccaatctga taaatcccaa ttcccagtgg gaagctcatc
      481 ccagcaatac caaatacctg ggtgagtgga tccagctacg ccgctggaaa tccaggggcc
      541 atcaatttct tgcatcggtt tgtaacacaa ttggatgcct tgggtgcttt caaccctgat
      601 ccggttgagc ttaaacagtt tcttgtcatt gattccgcca tccgtcacaa agatgtgtgt
      661 cggtctatgg ccctcatgtg gatggagact tcgaggttca tcccggggtt caatttcttg
      721 gcaggtacca tcatcctgaa taatgcccca gtccgtaaag cttccgggac aagtcgtaat
      781 atgtggattt tcgtagccta aatgaaaatg gctaacgctg agaggggtcc atattgggcg
      841 aacctcgaca tacataagcc cattacaggc ttggtgccac cagggggtat aggcaaatgt
      901 cgtttcggcg tatggatgaa gactgaactc tagaagatct aaggcatcgt aatccgagaa
      961 atctaccaaa cattcttcgg ccaggatcgg attattcgat gtttgcaaag cgtccaaggg
     1021 ggaagtggag ccactgcgcg ctcgtaagat attggcagtt gggtttacgg acggctttat
     1081 taaggtaggg agagaaggct ctttatgtcc agatttctga atggcaattc tgccttttgg
     1141 agttgccacc agaaccatgt gatttaattc ttgagaaact gccaaaacat tctggcttcc
     1201 tccggtcagg actagcacca aagccatacc aaatactcca gctcgaagta tttcgttcac
     1261 aaaatgtcta ttgggtgtcc ttcgcatcat cgtctccttc catcattctc agttaagttt
     1321 tgcagggatg tttcaagcca tctatggcta attctcttta tgttttccta cacagaaacc
     1381 ataaaaagca attcgtatgc cattgaagct acctattaga ataatcccta tcatcatttg
     1441 atttcacctg gttctatgca gcctggaggt agggggaagc tcggcacctt cgatatccgt
     1501 cagacattaa gaggagattg gtccgtatcg aaataggtaa aataggtatc tgtttggata
     1561 attgtctcgg agagttcaaa aggcaattgt caacttaagt caaatttggc tctatcccgc
     1621 attggccgtg cttacatagt ccaccgtatg ctccgctcgt ccaagcggct gcggcctttt
     1681 catcgcggga cccagaacaa ggcgtttgag aggccttgtt atggtgttgt atcggtcatt
     1741 cttgagaagg gacaaggagc ccccaactgg catctgccgc tttgtggaca gacttcgtaa
     1801 ggactctcac acccacaccc caaacgtcga tcaggaatct cgcggcaatc actctgaccg
     1861 gcgattgacc cgcaagttta tgtcggtgtg tgccacccca acgtcattca tttactttct
     1921 gaaggctccc atcatgcaaa ctttcttgaa cgtgggcgta gatgtggcca aagacgccgt
     1981 cgtggtcgcg tgcgctgagg acagctttct gtccacaggg tcacccatta gaaggctccg
     2041 gtctagtggg cacaggtatc caatggtgaa gggaccaatg gctctctacc aatgagatgt
     2101 gttgattcaa cccatgcgtg tctttgaggt ttgcggcagg aaccaatgca ctccccagtg
     2161 ccgtcgttat tcgacgacct ttcgcatgag caccaggttg tcgaggatca gattcgatga
     2221 ccagatgaag tcggcgtcgg ctatcgggtc atcga
//
LOCUS       DAORAQ010000348         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6870_length_2240_cov_68.400915, whole genome shotgun sequence.
ACCESSION   DAORAQ010000348 DAORAQ010000000
VERSION     DAORAQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_2240_cov_68.400915"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKM72_15100"
     CDS             <1..721
                     /locus_tag="PKM72_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62173.1"
                     /translation="EGMQSFNSSRFAEWSDFLLDVLGAVGALGLSFTYDQRFTGRVAI
                     WRVAPRKQLVQVGVGLLVVVALSPVLVWSYVYWDRDARFPLLVQFSAAWEMMLVNGRD
                     AAVQIVPPPLGWGKPQVDTVGLVLFYPKPYPGIRLREPYPDWKGFSHFHFEVYSELPE
                     AQSLIIRIDDAQHNNDYTDRFNQAITISPGLNHIHIPLDDIRLAPVGREMDLSAIKNV
                     RLFAINPPEEFSLYLDNFRLE"
     gene            1045..1521
                     /locus_tag="PKM72_15105"
     CDS             1045..1521
                     /locus_tag="PKM72_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62174.1"
                     /translation="MVANILTFSSAFRFRCFLWILVCVQLGLVGCHVTLISSYDPEMD
                     RTATSLQKKMDAFLTRLETHAGESQAHYSWDQVFYQDYLVQLRSLRLRAQSDPTNEFT
                     AKQLTLMMDNFEQLRLAHEAGPLPIPTIQATRDLFNQGWQAIIAKEIAKRRGMDSQ"
     gene            1664..1939
                     /locus_tag="PKM72_15110"
     CDS             1664..1939
                     /locus_tag="PKM72_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62175.1"
                     /translation="MPRPPFPTGPRPTPRMGGARPPAPPGQAKQFDSLRASLLFCPKC
                     QTATPARERLLLVLPTGSLYEYLCAHCGTSTGSKTDQAQSEVRLITP"
BASE COUNT          499 a          547 c          587 g          607 t
ORIGIN      
        1 ggaaggcatg caatcgttca attcgagtcg ttttgccgaa tggtcggact tccttttaga
       61 tgtgttggga gctgtgggtg ccctggggtt gtctttcacg tacgatcaaa gatttacggg
      121 acgtgttgca atatggcggg tggctcctag gaaacaactg gttcaggtcg gggtcggatt
      181 gcttgtggtg gtcgccctga gtccggtgct cgtctggtcc tatgtctatt gggaccggga
      241 tgcccgattc cctttactcg ttcagttttc tgccgcatgg gaaatgatgt tggtcaatgg
      301 gagggacgct gccgttcaga tcgttcctcc gcccttaggc tggggtaaac cacaggtcga
      361 taccgtgggg ctcgtgctgt tttatccaaa accctatcca ggtatccgcc ttagggagcc
      421 ctatccggat tggaaaggat tttctcattt ccattttgag gtgtattccg aattgccgga
      481 agcccaatcg ctgatcatcc gaattgatga tgcacaacat aataacgact atacggaccg
      541 gtttaatcag gccatcacca tttcgccggg cctcaatcac attcacattc cgttagatga
      601 cattcgcctc gcccccgttg gtcgggaaat ggatctcagc gcgattaaga atgttcgact
      661 ttttgccata aacccaccgg aagaattttc cctgtatctc gataatttcc gtctggaata
      721 gagttgtcag ggttcgtgga gtggaagccg ggcgagcgaa catacagagt atcccaataa
      781 ttggcagatg catctgtgaa gtcggcgctg gcatctaagt caacaatcct gtgtggcggt
      841 tgagaatatg aggttgggtt agggtcgatt ttttcattga agcccggtag acagatgcgc
      901 acaggtgagt agaatatatt ttgatgaagc tgtttcattt ttcaatatgg cgcgtcattc
      961 gactgggaag gaatgggttg caaaactatt catgtccggt ggctatcccc accatggaat
     1021 ctcaacaaaa aggcgattat cccaatggta gctaacatcc tgacgttttc ctccgcattt
     1081 cgttttcgat gttttctatg gattttggtg tgtgtgcaac tgggtctggt tggttgccac
     1141 gtcacgttaa tttcttctta tgatccggaa atggatcgga cggcaacatc gctccaaaaa
     1201 aagatggacg cttttttaac ccgactcgaa acccatgccg gggaatccca ggctcactat
     1261 tcatgggatc aggtctttta tcaggactat ctggtccagc ttcgctccct gcgactgcgc
     1321 gcacagagcg atcccacgaa tgagtttacg gccaaacagt tgacgctcat gatggataat
     1381 tttgagcaac tccgtttggc ccatgaagcg gggcctcttc ccattcccac gatacaggcc
     1441 acacgagatt tatttaatca aggctggcag gccattattg cgaaagaaat cgccaagcgg
     1501 cggggaatgg actcacagtg atcatatgca gtgacaggaa gcacgggtgg gtggttgtca
     1561 cgcgtgagtg gtgtggcttg aatgttgggt catgcctcta tggtcttcct caatgatgcg
     1621 aagcgatctt gaccgagtca tggcccttcg ctaagatctg cgtatgccta gaccgccatt
     1681 tcctacaggt cctcgaccca caccacggat gggtggggct cgtcctccgg ctcctccagg
     1741 ccaagctaaa caattcgact cgctccgggc gtcgttgctg ttttgtccaa aatgtcagac
     1801 tgcgactccg gctcgtgagc gtcttctttt agtgctccca accggaagtc tctacgaata
     1861 cctctgtgcc cattgcggga cgtccaccgg ttctaaaacc gaccaggccc aaagcgaagt
     1921 tcgcctcatc actccctaaa tttctcttgc atcgtttttt gacggaggat tttttgtgct
     1981 atggctccgt ccttcccgtc tttccatttc gtcatcatcg cctgtaggta gtggggagtc
     2041 atcttcttga ggatatgcaa cgaaagaaaa aggtatgcca tttcaaggag gcagaaggat
     2101 ctggctggac ctcaaggtgt ttcgacctgg gcacagattc ttcgccgtgg gctcagaatg
     2161 acaatgtgca atcaggtggc cccgcaagta ctaagaggag agccaaattc gacagaatta
     2221 cgccaagatt gattcccaat
//
LOCUS       DAORAQ010000349         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6922_length_2229_cov_69.782429, whole genome shotgun sequence.
ACCESSION   DAORAQ010000349 DAORAQ010000000
VERSION     DAORAQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6922_length_2229_cov_69.782429"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..513)
                     /locus_tag="PKM72_15115"
     CDS             complement(214..513)
                     /locus_tag="PKM72_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNP62176.1"
                     /translation="MKQKLMRYMAGNFSCQEIAQLITDYLDGSLNLGERVRFQIHLGL
                     CFACRNYLRQMKHTIATLRQLPPEPVPQHVKEELLERFRNWKQHQHQSSVKSENE"
     gene            complement(531..1208)
                     /locus_tag="PKM72_15120"
     CDS             complement(531..1208)
                     /locus_tag="PKM72_15120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNP62177.1"
                     /translation="MPISPSPHTLHDTLKLDEPGLVRALQAGDEKVFAAVMDWYSGSL
                     LRLALSFVPSRAVAEEVVQETWMGVFEGIHRFESRSSFKTWLFRILTNRAKTRGIRES
                     RYEPFGLSASSGEIDEGLSLEDSLFITEGSRTGHWKDPPNAWEPDTPERALLSKECRA
                     AIETAIESLPATQRQVMTLRDIEGVGSEEICNILGISETNQRVLLHRARTKVRRELHP
                     YVQGDNT"
     gene            complement(1452..2093)
                     /locus_tag="PKM72_15125"
     CDS             complement(1452..2093)
                     /locus_tag="PKM72_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNP62178.1"
                     /translation="MSRRSIGIPCTTVPKSTDGGGLSRLQEIADRRAIPGVLLFSGSG
                     DVLFMNPEAEALNRQIMGDAYDAEARGVWPAEVLELCGSLRTLLDSPENQMAEGQHEL
                     RRVTGDGKSPVLLRGFPLAAHTEKDEACMLIIMEKIGRERRVVPNQAKEQYRLTSREV
                     EIVKYISEGWTNKEIARHLEISEHTVKEHVRHLLKKTKTTTRTGILAQIFQDT"
BASE COUNT          565 a          595 c          545 g          524 t
ORIGIN      
        1 gacaccagat gaccggtccg gacatccggc agttcaaagt gaggcaaggg cgtccccagc
       61 gggagcatcg ttgaatcttt caaggccatg aatgtctcct ttaatgaaaa caggacgatt
      121 aacacacgaa gggttgtgac actccataag ccttatccac cacattgggt cgaaagggag
      181 aaggagaaat cgcacagacc atcatccggc agattattcg ttttcagatt tcacggaaga
      241 ttggtgttga tgctgtttcc aattccgaaa ccgttcaagc agttcctctt ttacatgttg
      301 ggggacgggc tcaggaggca attgtcgtaa ggtcgcaatg gtatgtttca tctgacgtaa
      361 ataattacgg caggcaaaac acaagcccaa atggatttga aatcgcactc gctcgccaag
      421 attcagggac ccatccaggt aatccgtgat aagttgcgcg atttcctggc aggaaaaatt
      481 tcccgccata tatcgcataa gtttctgttt caaattcgta tccattgtgg ttacgtgtta
      541 tccccctgaa catagggatg gagctctcgg cgcactttag tgcgcgcccg gtgtaacagc
      601 acccgttgat tggtctcact gatcccaagg atgttacaga tttcttccga accgacaccc
      661 tcgatatcgc gcagagtcat cacctgccgc tgcgtagccg gcaaactttc aatcgcggtt
      721 tcaatggctg ctcgacattc cttcgagagc aacgcccgtt ctggagtatc cggttcccac
      781 gcgttagggg ggtctttcca gtgacccgtt cgggaaccct ctgtgataaa caaggaatcc
      841 tctaaggaaa gaccttcgtc tatctctccc gaagacgcac ttaatccgaa cggctcatag
      901 cgactttccc gtatccctcg cgttttcgcc cggttcgtca aaatccggaa gagccaggtc
      961 ttaaaagacg atcgactttc aaaccgatgg atgccttcaa acacccccat ccaggtttcc
     1021 tgaacgacct cttcggccac ggcccgactc ggcacaaagg acaaggccaa ccgcaacaac
     1081 gaaccggaat accaatccat cacggccgcg aatacctttt catctccagc ttgaagagcc
     1141 cgaacgaggc ctggctcatc aagcttcaac gtatcgtgca gagtgtgggg agagggagaa
     1201 attggcaacc tctgagagga tcaagagaca tcaaacacga accatgagat tatcctcagc
     1261 ttgagagtga caatcaaggg tttcctgaga agaaccctcg gataggaaac cggtcattct
     1321 aaacaggcac aagaaactag taggaaaaca tagccaggca ctgcagattg acagtcaaac
     1381 ggggccatgg aacattgtag gatcgaaggg aaaaaaggga gaaggggcag tcgggcggac
     1441 gcctggaggc gttaggtatc ctggaagatt tgggcaagaa tgccggtgcg agtggttgtt
     1501 ttcgtttttt tcagaagatg gcgaacgtgt tctttcacgg tatgttcgct tatctccaga
     1561 tgtcgagcaa tctctttatt ggtccacccc tcactgatat atttcacaat ttctacttcc
     1621 cgactcgtca aacgatactg ctctttggcc tgattaggca ccactcgacg ttcacgtccg
     1681 attttctcca tgatgatgag catacaggct tcatcttttt ctgtatgggc tgctaatgga
     1741 aaaccacgca gcaatacagg cgatttccca tcacccgtca ctcgtcgtaa ctcgtgctgg
     1801 ccttcggcca tctggttctc gggactgtct aataatgtcc gaagggaccc acataactcc
     1861 aggacctcgg cgggccacac acctcgcgcc tcggcatcat acgcgtctcc catgatctgg
     1921 cgatttaacg cctctgcctc ggggttcata aacaacacgt ctccggatcc tgagaagagc
     1981 aacactccgg gaattgctcg tctgtcggca atctcctgga gccgggacag accacctccg
     2041 tcggtggact ttggaacagt cgtgcaagga attccgatag atcgtcgact caaggtgtac
     2101 tccgtggatc aggttatgca gcgatgaaca atctcctcac agccatctgg aaaggggcaa
     2161 caactcacgg tgcaaatgca cccctaccgc tacctatgat cttattgaac tgttgtcgga
     2221 ttgtggcag
//
LOCUS       DAORAQ010000350         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_6972_length_2218_cov_68.003236, whole genome shotgun sequence.
ACCESSION   DAORAQ010000350 DAORAQ010000000
VERSION     DAORAQ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6972_length_2218_cov_68.003236"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PKM72_15130"
     CDS             complement(<1..1213)
                     /locus_tag="PKM72_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNP62179.1"
                     /translation="MSNFNPQLTLAPSPLPTEPNGAPRGFLTLRAKFSLFVSLVIVLA
                     CSSLSGYLIQQEAEVMKAALLKTGTILVKTLNNVSVNRLIIQDTHYLETMLDGALSAP
                     EVVYAIARDPDGHVLVGKSKGKLVNTTKLTRDETQPLFPDDSLTEGLITPTSQNFIIE
                     PILTVLHTSPLKKGLLSDRSAERKGAEGFRIGSETIYDFALPVYRQDRRLSTMELLLS
                     ENLQDRLTVSPKPSGIIGIIQIGISTAHMQQSLNQTVWHIGLLTVVIILVGILLTILL
                     ANRIVTPLHRLVEASQKIAEGDLAVTVTADTQDEVGQLTTSIDRMARALQQREEAISH
                     YVHTITKQVTQLSTLHQTGIVITSTLDVQKLFSTVLKLLRKNLGFQRMILILQDPGST
                     KCMISDVSGVPP"
     gene            complement(1200..2090)
                     /locus_tag="PKM72_15135"
     CDS             complement(1200..2090)
                     /locus_tag="PKM72_15135"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNP62180.1"
                     /translation="MGIFLNLSTVSLAEEVVILKSADINAYSEAISAYTSALPPSFRV
                     TGEYDLQGDMAKGRILSQRIRASNANVVLAVGLKAALSAKLDILDIPVIFCLVLDPEK
                     YGLPTANMVGLSLNVPFRQHLKSLHTLTPKASRIGVLFDPQKTNEMHSQLLHDAKTLG
                     ITIVSKEVHQEQDIASALNALKGHIDALWLLPDSTVLTENTLDFLLSTTLEAKIPVIG
                     FSAGLVRSGAVVGAYFHYEDIGKQAAQLTQQLAGQTTSSLLGTIIPPERVHQSINQKS
                     ARYLSLSLTPDILRQFDEQF"
BASE COUNT          544 a          465 c          620 g          589 t
ORIGIN      
        1 ccggaggtac cccggacaca tcagagatca tgcacttggt gcttccaggg tcctgaagaa
       61 tcaggatcat acgttggaat cccagatttt ttcgaagaag cttcaggacg gtggaaaaca
      121 gtttttggac atctaaggtc gaggtgatca caattccggt ctggtgcaag gtgctgagtt
      181 gtgtgacctg ctttgtgatg gtgtgaacat aatgggagat ggcttcctca cgttgttgaa
      241 gggctcgtgc cattcgatcg atactggtgg tgagttgtcc tacttcatcc tgggtgtcag
      301 cggtaaccgt cacggccaga tccccttcag cgattttttg tgaggcttcc accaaacgat
      361 gaagaggcgt aacgatgcga ttggctaaca gaattgtgag aagaatgccc acgaggatga
      421 ttaccacggt gaggagaccg atgtgccata ccgtttggtt gagggattgt tgcatgtgtg
      481 cggtgctgat gccgatttga ataatgccga tgatgccgga cggtttaggt gataccgtca
      541 aacggtcctg aaggttctct gacaacaaca attccatggt ggagagccgt ctatcttgcc
      601 ggtagaccgg aagggcgaaa tcataaatgg tttctgatcc gattctgaat ccttctgcac
      661 cctttcgttc tgcggatcta tcagagagaa gtcctttctt gagtggtgag gtatgcaaga
      721 ctgtcaaaat tggctcaatt atgaaatttt gggaagttgg cgtgatcagt ccctcagtga
      781 gggaatcatc cgggaataag ggttgcgttt cgtcacgagt gagcttcgtc gtattcacta
      841 attttccttt tgattttccg actaacacgt gaccgtccgg gtctcgtgcg atggcgtaga
      901 cgacttccgg tgctgaaagc gcgccatcta acattgtttc cagataatgt gtatcttgaa
      961 taatgaggcg attgacgctg acattgttca gtgttttaac cagaatggtt cccgtcttga
     1021 gtagcgcagc tttcatgacc tcagcctcct gctgaatgag atatccactg agacttgagc
     1081 aggcgagaac gataaccaga ctgacaaata aggaaaattt cgcgcgtaac gtgagaaatc
     1141 cacgcggtgc cccattgggt tcagttggga gaggcgatgg cgctagagta agttgggggt
     1201 taaaattgct catcgaattg cctcagaatg tctggagtga gagagaggct caagtacctc
     1261 gcggacttct gattgatgga ttgatggacg cgttcaggag gaataatcgt tcccaataaa
     1321 gatgaagtgg tttgtccggc cagttgttgg gtaagctgag cagcctgttt gccaatgtct
     1381 tcataatgga agtatgcgcc gacaactgcg ccgctacgca ctaatccggc ggaaaaacct
     1441 atgaccggaa tcttggcttc caacgttgta ctcaacagaa agtccagagt gttttcggtg
     1501 agaacggtac tgtctggtaa cagccacaag gcgtcgatgt ggcctttgag ggcattgagt
     1561 gcggaagcga tatcctgttc ttgatgtact tctttggaga cgatcgtgat gcccaatgtc
     1621 ttggcgtcat ggagaagttg actatgcatc tcgtttgttt tttgcggatc gaagagcact
     1681 ccgatgcgtg aggccttggg tgtcaaagtg tgtagggact ttaggtgttg tcggaatggg
     1741 acattcaaag agagaccgac catgttggcg gttggcaagc catatttttc cggatcaaga
     1801 accagacaaa atatgacagg gatatccagg atatcgagtt tggcagaaag agctgctttt
     1861 aatcccacgg ccagcacgac attggcatta gatgcgcgaa tccgctggga tagaatgcgg
     1921 ccttttgcca tgtctccctg gaggtcgtat tccccagtca cacgaaaaga gggagggaga
     1981 gccgatgtat aagcggaaat ggcttcagaa taggcgttga tgtctgccga ctttagaatg
     2041 acgacttcct cggcaaggga cacagtggac aggttaagga aaattcccac acacaagctc
     2101 acccggataa actgcatcca atgaggtgat cggtgggata tatcccttaa attgttcatt
     2161 atttctcagc gacgattatg aggtaattcg ttctagattt accttgtttt cgcctcgg
//
LOCUS       DAORAQ010000351         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7116_length_2188_cov_87.706517, whole genome shotgun sequence.
ACCESSION   DAORAQ010000351 DAORAQ010000000
VERSION     DAORAQ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7116_length_2188_cov_87.706517"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKM72_15140"
     CDS             complement(<1..248)
                     /locus_tag="PKM72_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62181.1"
                     /translation="MSDLNTIVNFNMALAKETENRTLDVQVLESGVKALLKDAGKGWY
                     AVAEKSFQSGRLIVVGQILITFEWSDWRNGNFWWLQSV"
     gene            complement(301..939)
                     /locus_tag="PKM72_15145"
     CDS             complement(301..939)
                     /locus_tag="PKM72_15145"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNP62182.1"
                     /translation="MNGLSDHTFKRYFLTGLFLIIPAWGTLLILYTLLEALEHMTVDI
                     VWALYGVRIPGSGITFFCLLVLWVGMGTTHLLGQRIHRKLENSLERIPFVRSIYYTLK
                     SMADVIKFRDRFGQSKVVAFPFPRDGLWALGFDMGLPPHKLQVAPEGPLMMVFVPTAI
                     HPFTGYLAFVPEGSVNRIQLRPEEAMKMEFSAGLYRPQPGWLSAANFAKKDT"
     gene            1139..1441
                     /locus_tag="PKM72_15150"
     CDS             1139..1441
                     /locus_tag="PKM72_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNP62183.1"
                     /translation="MRPENTSTGKGEIAADLLAILCCPETKQEVCLLDQTIVERLNKR
                     ISKGELKTKGGRPVTEQIDGGLLRTDRTVAYPIRDQIPIMLIEEGIILEKSDLSSI"
     gene            complement(1796..>2188)
                     /locus_tag="PKM72_15155"
     CDS             complement(1796..>2188)
                     /locus_tag="PKM72_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62184.1"
                     /translation="NFFCEERIMNSILHRAVASFYNLIYEGQAFASTMARIDAAEQER
                     YFGRVEGLNWVLDRCQELEDVDMNLTTASLQKLLGDVKSDLEHELSIQRREKGRRADG
                     REEALIFVTDYLISLITATEIESTKSPV"
BASE COUNT          650 a          540 c          453 g          545 t
ORIGIN      
        1 tacacacttt gaagccacca aaaattcccg tttcgccaat cgctccactc aaaggtaatc
       61 aatatctgtc caactacaat gagtcggcca ctttgaaaag acttttctgc aaccgcatac
      121 catcccttac cagcatcttt aagaagtgcc tttacaccag actcaagaac ctgcacatcc
      181 aacgttcgat tttcagtttc tttggctaaa gccatattga aatttacaat agtattcagg
      241 tcagacatcg ttgctcgcct gataaagact ggggatggtt tattcacttc tgctgacagg
      301 ttaagtatcc ttcttcgcaa agtttgctgc tgataaccat cccggttggg ggcgatagag
      361 tccggcagaa aactccattt tcatggcctc ttccgggcgc aactgaattc gatttacgct
      421 tccttctggg acaaaggcca gatagccggt aaagggatgg attgctgtcg gaacaaagac
      481 catcatgaga ggtccttcgg gagcaacttg cagcttatgt gggggtaatc ccatgtcaaa
      541 acctaaagcc caaagcccgt cacgaggaaa cggaaatgcg acgaccttgc tttgaccaaa
      601 ccgatcacgg aacttgatga catccgccat actttttagg gtatagtaaa tactacgcac
      661 gaatggaatc cgctctaaag aattttctag ttttcgatga atccgctggc caagtaaatg
      721 ggtcgttccc atgcccaccc agaggaccag aagacaaaaa aaggtaatcc ctgacccggg
      781 aatcctcact ccatataagg cccacacaat gtctaccgtc atatgttcta aggcttcgag
      841 tagcgtatag aggattaata acgttcccca tgccggaata attagaaata agccggtaag
      901 gaaataccgc ttgaaggtat ggtcagaaag tccattcata caatgaaaat agggcggtca
      961 ttgaaattga gagcagtcta gcaaaaacct acccactccg cagcaccagc ggcgcgactg
     1021 cggattgctc acgattccga atttcctgag aatttcttgc tacaaacgaa ggtgggtgct
     1081 actgttctaa tgaaaaaagt gagtcaattt attggtacgt attgaaagga gccatagagt
     1141 gcgacctgaa aacacatcca caggaaaagg cgagattgct gctgacctat tggccattct
     1201 ctgttgtcca gagaccaagc aagaggtgtg tctattggac caaacgattg tggaacgact
     1261 gaataaacga atttcaaaag gagaattaaa gactaaggga gggcgacccg tcactgaaca
     1321 aatcgatgga ggactccttc gaacagatag aacagtagcc taccctatcc gcgatcaaat
     1381 tcccattatg cttattgaag aaggcatcat cttagagaaa tctgatcttt catccatcta
     1441 aggccaaggc cccacaaatt ctgcaaaaaa gccgtaattg tttttgtgga atggactttt
     1501 tatgaacctt gagtgctccg accgcctttt ctaacccacc atagcgatcg accaatctgg
     1561 actacaacat agataaacac accaaaaaaa atcccatagt atccatacga ttcaatcggc
     1621 caattctgtc ctccatgcaa gagaaagccc aaggccacat gagctcctaa ccctaaacat
     1681 agggctaaga taatccattc acggaccatt gtttttcggg acaaccccat gctagttccg
     1741 agtacaaaga gcagtttgga ccatcccact aatggcccaa ctggtaggta ggcaattaca
     1801 cagggctttt cgttgattcg atttccgtgg cagtgatcaa gctgatcagg taatcagtta
     1861 caaaaattag cgcttcttct ctcccatcag cacggcgccc cttttccctg cgttgtatgg
     1921 agagttcgtg ttccagatct gacttgacgt cccctaaaag cttctgaaga gaggccgtag
     1981 tcagattcat atccacatcc tctaattctt gacatcggtc caggacccaa ttgagccctt
     2041 ctacccgacc aaaatacctt tcttgttcag ctgcatcgat tcgggccatg gttgaggcaa
     2101 atgcttgccc ttcataaatg agattgtaaa aactcgccac ggcacgatgt aaaattgaat
     2161 tcattatccg ttcctcacaa aaaaaatt
//
LOCUS       DAORAQ010000352         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7163_length_2178_cov_57.678285, whole genome shotgun sequence.
ACCESSION   DAORAQ010000352 DAORAQ010000000
VERSION     DAORAQ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_2178_cov_57.678285"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKM72_15160"
     CDS             complement(<1..389)
                     /locus_tag="PKM72_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62185.1"
                     /translation="MRNFSQHRNIFGIALLIGGLAGCFLTVEKSEAQKLPFFDRNVVL
                     LDCVESATNTFVVENMSSTVDVEINRGLDCAAAVQKLFVKGYVLGPGAGGVTSGDANS
                     LDHFLMLFLLNPNDLLERRIDTSTLNPS"
     gene            complement(987..1334)
                     /locus_tag="PKM72_15165"
     CDS             complement(987..1334)
                     /locus_tag="PKM72_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62186.1"
                     /translation="MESLVFFAVLLFLSGLALAIRWLNERIRRDIAFKNLKDLFLPSN
                     DSHEQDDRKAAATTKTSPTKPQQAVQQFRPITSPKRRTASQLRLRNRADLRQGIVIMT
                     VLGPCRALQHPDD"
     gene            complement(1348..>2178)
                     /gene="floA"
                     /locus_tag="PKM72_15170"
     CDS             complement(1348..>2178)
                     /gene="floA"
                     /locus_tag="PKM72_15170"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNP62187.1"
                     /translation="GMRLRRVPPVTVINARISAVKAGLEIPVNDLEAHFLAGGNVVKV
                     VNAMISADKANIPMTFKRAAAIDLAGRDVLEAVKMSVLPKVIETPRVTAVAKDGIQLI
                     AVSRVTVRANIDRLVGGAGEETIIARVGEGMVSTIGSSASHKDVLENPDYISKNILGK
                     GLDAGTAFEILSIDIADVDVGENIGAKLQIDQANADKQIAQAKAEERRAMAVALEQEM
                     RARVVEAEAEVPRAMAEAFRQGNLGIMDYYRMKNVQADTSMRDAIAGTDEESSGPARK
                     "
BASE COUNT          501 a          578 c          542 g          557 t
ORIGIN      
        1 gaggggttta aagtcgaagt gtcgatcctt cgctcaagca aatcattggg gttcaggagg
       61 aataacatca agaaatgatc cagggaattt gcatcacccg aggttacacc tccggctcca
      121 ggccccaaga catacccttt gacgaatagc ttttgtaccg ctgctgcaca atccagtcct
      181 ctattgattt ccacatcgac ggtcgaagac atattttcaa ccacaaaggt attggttgcg
      241 ctctcaacgc aatccaaaag aacaacattc cgatcgaaaa atgggagctt ttgcgcctca
      301 gatttctcta cggttagaaa gcatcccgcc agacctccaa ttaacagcgc tattccaaag
      361 atattcctgt gttgtgaaaa gttcctcatt gggtcctcca taggtgtaat tgtaagagcc
      421 atacccgaac acttctgaag catgttgact cagctaaccg tgattgacgg ttgttcttct
      481 tggcctttca gctctcgctc cggcttgtgg atgtaatgaa accagatccg gttcaatcgt
      541 gggtgatgca tggatgaaaa cgaggaattc ctcctttgct gaaatagaac gaaaaaaggg
      601 cctttgaggc ttacatattc ctcaaaggcc ctccattgcc ttgccctatg tgctcaaccc
      661 acccatgaac tggttctagg atggttgaat gggatttcta ccacaatctg aataagaatg
      721 tcccgccctg tttaggccgg cgggttggat tcaaagagaa cgccgttcct tgggtccgta
      781 tagcgtatgg taactttctc ttgatgattt ccctcgccgg cacccttgtc ccaatagggg
      841 cgagcgaccc ttcaagactt atcgttcaaa attgcaattc tttagttcca tagacaggtt
      901 ggtgggatga gggccaatgg gtaacaacgc cgatggctgc ccattcaact tcagccaggg
      961 cagcctttca aagccatagc attgctctaa tcatcggggt gttgcagcgc ccggcagggg
     1021 ccaagaacgg tcatgataac aattccctgt cggagatcgg cccgattgcg taaacggagt
     1081 tggctggcgg tccgccgttt tggagaggtg attggtcgga attgctgtac ggcttgttgg
     1141 ggttttgtcg gggacgtttt tgtggtggca gccgccttac ggtcgtcctg ttcatgggaa
     1201 tcattggatg gaagaaaaag atccttaaga tttttgaagg cgatgtcccg ccgtattcgc
     1261 tcattcagcc atcgaatggc caaggcgagc ccggataaaa aaagcaggac agcgaaaaac
     1321 accaaagatt ccatgctcac catcctctca ctttcttgct gggccggaag attcttcatc
     1381 cgtcccggca atggcgtccc gcatggacgt atcggcttgc acatttttca tccggtaata
     1441 atccatgatg cctaagtttc cctgacgaaa tgcttcggcc atggccctgg gaacctctgc
     1501 ctcggcttcg acgacacggg ctcgcatttc ctgttccaag gcgaccgcca tcgcccgccg
     1561 ttcttccgct ttggcctggg caatttgttt gtccgcattg gcctggtcga tttgcagttt
     1621 tgcgccaata ttttcaccga catcgacatc cgcaatatca atggacaaga tttcgaatgc
     1681 tgtgccggca tcgagtcctt tccctaaaat attctttgaa atgtaatccg ggttttccag
     1741 aacatccttg tgagaggcgg aagatccgat cgtgctaacc atgccttctc ccacgcgggc
     1801 aatgatcgtc tcttcacccg ctccgccgac tagccggtca atattggcgc gaacggtcac
     1861 ccgggacacc gcaatgagct gaatgccatc cttagcgaca gcggtcaccc gaggtgtttc
     1921 gatgactttg ggaaggaccg acattttgac cgcctccagc acgtcacgac cggcgaggtc
     1981 aattgcagct gcgcgtttaa acgtcatggg gatattggct ttgtcggccg agatcatggc
     2041 attgaccact ttgaccacat ttcctccggc cagaaagtgg gcttccaaat cgttcacggg
     2101 aatttcgagt ccggccttca ccgcgctgat tctggcattg atcacggtga ccggaggaac
     2161 ccggcgcaat cgcatgcc
//
LOCUS       DAORAQ010000353         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7235_length_2163_cov_68.406546, whole genome shotgun sequence.
ACCESSION   DAORAQ010000353 DAORAQ010000000
VERSION     DAORAQ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_2163_cov_68.406546"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKM72_15175"
     CDS             complement(<1..1061)
                     /locus_tag="PKM72_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNP62188.1"
                     /translation="MMSQSQLAQKWNGRRVGIFTFIGMALLIAGICLGLGIAGDSSLL
                     PGLSPQLKVSGTPLSDSSSQPLVSGALSFGQQDAQAQSTALIRVAKLARPAVVNISTT
                     GTAKSPETLRSPFFDDPFFRRFFGDEFERRFEPPPSPRQEGGGSGVIVSDDGYIITNN
                     HVIENSDAIEVLLSDKRTFSAKVIGTDPKTDLALLKIDAQDLPVLPWGDSHQLQVGEI
                     VMAVGNPFGLNQTVTMGIVSAVGRANVGIVDYEDFIQTDAAINPGNSGGALVNLQGEL
                     IGINTAIFSRSGGYMGVGFAIPSNMAKSIQASLIEHGKVVRGWLGVSIQDLTPDLQTQ
                     FDTPDTQGALVSEVMEDSPA"
     gene            1848..2021
                     /locus_tag="PKM72_15180"
     CDS             1848..2021
                     /locus_tag="PKM72_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62189.1"
                     /translation="MSLHQAIKSLIIGALVVLGVSLGQHLWAKEPTKNQITNGSTKHE
                     GVVSSVQSEVEST"
BASE COUNT          623 a          505 c          505 g          530 t
ORIGIN      
        1 gcagggctgt cttccatcac ttcgctgacc agggccccct gcgtatcagg agtatcaaat
       61 tgggtttgga gatctggggt gaggtcctga atcgaaacgc ccagccatcc tctgaccacc
      121 ttcccatgtt caataaggct ggcctgaata gacttggcca tgttgctggg aatggcaaaa
      181 ccaacgccca tatatccccc ggagcggcta aagatggcag tattgatccc gatcaactct
      241 ccctggagat tcactaaggc cccacccgag ttaccgggat tgatcgccgc atcggtctga
      301 atgaagtctt cataatcgac aatccccaca ttggcccgac ccaccgcact gacaattccc
      361 atggtgacgg tttggttcag cccaaatgga ttgccaacgg ccatcacaat ttcaccgacc
      421 tgtaattgat gggaatctcc ccatggcaag acaggcagat cctgggcatc tattttgagt
      481 agagccaaat ccgttttggg atcagtgcct atgaccttgg ctgaaaaggt tcgcttatcg
      541 gataacagca cttcaatggc atcactattt tcaatcacat gattattcgt gatgatatac
      601 ccatcgtcac tcacaatgac tcccgaacct cctccttctt gacgagggga gggaggcggt
      661 tcgaaccgcc gttcaaattc atccccaaaa aatcgacgga agaagggatc atcaaaaaat
      721 ggtgatcgca atgtttccgg acttttggcg gtgccggttg tggaaatatt gaccacagcg
      781 ggacgcgcca gcttggccac ccgaataagg gcggtgctct gagcctgggc gtcttgttgc
      841 ccaaatgaga gtgcaccaga cactaagggt tgtgatgagg aatccgaaag aggcgtcccg
      901 ctaactttca attgtggtga gaggcctggc aggagactgg agtccccagc gattcccagg
      961 cccaaacata taccagcgat gagcagggcc atgccaatga aggtgaagat acccacgcgc
     1021 cgtccattcc atttttgagc tagttgggat tgactcatca ttggacctca cgaagaaaat
     1081 tcgactatgt gaaaagaact tcatacacgc agtcttgttt cacatcaaga atgttcaagc
     1141 ctaaaggttc taaaataaat aaaagtttat ttctgtttcc tgactagttc attcaataaa
     1201 tccaaggaac catttcagaa tcagcgcatc gataaatatg atcgcgacaa tacaacccat
     1261 ggtaagcaat tcaaacatcg gacacctccc tttatttgat acataggggt tggagccggt
     1321 tgtatcccga atgcgtggaa tgctcctcca tgtctttcaa ataagactaa aaattcctat
     1381 ggtcaagagg tgggagtcct gaaaattccg ttccacggga acgttcgaaa tcctatatcc
     1441 ggactatatc tatattgatg taggcgccca tggtttatga atttagacag ttctaaggcc
     1501 tgcacagatg ccttttctag cacacactgg ttacgaaacg ccctaattgc ctaattctgc
     1561 tgaacatttt tccttaaaat caccatggaa cattgccggt ccgaagcagt ttcttgactt
     1621 gatataaaaa atgattatcg aaattttaat tgaagacgat actgtggatt cttggaagcc
     1681 gttttcctcc acctctaatc agagagggat aagccatatt gagcaaagga gagtggtgaa
     1741 tggtttcctg aatacaaaga cccttcgagg atcaagcaca gaatcgtaga accatacgga
     1801 tttcatttaa aattatgtca gagaggaagg ttaagaaagg aggcgacatg agtcttcatc
     1861 aagctatcaa aagtctcata ataggagctc tcgtggtgct gggtgtaagt ctggggcaac
     1921 acctatgggc gaaagaaccg actaaaaatc agattaccaa cggatcgacg aaacatgaag
     1981 gggtagtttc ttctgttcaa tcagaggtgg aatctacatg aaaagaagaa gtcgtaaaaa
     2041 agcggaaagc tttggtaaaa gaagccctca atgctctttc agagaccaag aaagcgttga
     2101 agtcttttga agatggcaac accaaagatg ctctggccgc attggccgaa gtgaccggac
     2161 aat
//
LOCUS       DAORAQ010000354         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7236_length_2163_cov_56.148482, whole genome shotgun sequence.
ACCESSION   DAORAQ010000354 DAORAQ010000000
VERSION     DAORAQ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7236_length_2163_cov_56.148482"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKM72_15185"
     CDS             <1..186
                     /locus_tag="PKM72_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62190.1"
                     /translation="GTFLTGKVPKAILAVACPNELPSRYADTGGVQTRGAQPESARRA
                     QTVPAFAPVTVARLARP"
     gene            1047..1853
                     /locus_tag="PKM72_15190"
     CDS             1047..1853
                     /locus_tag="PKM72_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62191.1"
                     /translation="MNKNLIPLLSAIILTLLCGAHTYAEQNAEELAKKTQNPVADLIS
                     VPFQNNLNFGLGPHNRVQNILNIQPVVPFNLTENLNLITRTIAPIIRQPDLTQDSGDT
                     NGLGDINISLFFSPAKSGQVIWGLGPILQFPTATDEVLGTRKWSAGPTGVALTMQGPW
                     VVGALANQIWSYAGNDDRDKVSAFLFQYFINYNFKGGLYLTSAPIITANWKATSGNKW
                     VIPFGGGIGKVFRIGKMPLNAQTQAFYNVQQTTFGPDWTLRLQVQFLFPK"
BASE COUNT          551 a          535 c          509 g          568 t
ORIGIN      
        1 ggtactttcc ttacggggaa agtacccaaa gccatattgg ccgtggcctg tccaaatgag
       61 ttgccttcgc ggtacgccga caccggcggt gtgcaaactc gcggagctca gcctgagtcc
      121 gctcgaaggg ctcaaacagt gcccgccttt gctccggtga cggttgcacg gctggccagg
      181 ccataaggcc aacgaagact gcgaaagcaa tgaatccttg tttatcgtgg aaaactaact
      241 cgaagtgcct gtcctgagtc tttgggaatg actcggacaa ggtcggtctt tagattcaag
      301 cgtccgaccc ttgggtcaat cagtaggcgt gggaaagtgg aaagagaaaa gggtgttttt
      361 gcctgataag ttggcagaga tgattgcaat tgctcgttca atctaaccga tcgctgtaga
      421 cggttacatt tttagactat ttctgttgcc ctggttctat tttcgctttg gcaaaaagca
      481 aaaattggat gcgtgcgaca gtccgctccc gacccaaagc ggtcatactg ttgatgtaat
      541 tgtgagcctt ttgaatttta atcagcggac cacaggttcg atccttgcac ggcccatcaa
      601 ttcaatcaac cactaactcg tccacgccaa aaccccattc gtggaaaaac ctgttataaa
      661 cttgtttctg gaaattgggc ggcggcgtta tcctgtatta caagagtggg tgtagagatt
      721 tttgaccata tggcgagaat ttctggccct atggcatcga ggccaaccga accacgttag
      781 aagccttatt ccagtattcc tttgagcagg gcatggccac acgccagctg acggttgaag
      841 aacttttcca tggatcaact cttaagctaa ttgagaaatg atcatcatgt gtgttttgat
      901 gattcccttt gtagctattt attctagggt gcaggtgatc ttgtaaatga ataaggaaca
      961 tatacatagc gaaactttca tcttgatatg ggaaaaatcg gttaatattc tctgataacc
     1021 gcaatattct atatagtgag gtgtacatga acaagaatct gattcccctt ttgtctgcca
     1081 tcatactcac gctattatgt ggagcccaca cctatgccga acaaaatgcc gaagagttgg
     1141 ccaagaaaac gcaaaacccg gtcgccgatc tcattagtgt cccctttcaa aataatttga
     1201 attttgggct gggtccgcac aatcgggtac agaacatctt aaacatccaa ccagttgtgc
     1261 cgtttaacct gacggagaac ctcaacctca tcacccgcac catcgctcct atcatccgcc
     1321 agcctgacct cactcaagat agcggggata ccaatggcct gggcgacatc aatatctcct
     1381 tgtttttttc tccggctaag tctggccagg tgatctgggg gcttggcccc atcctgcaat
     1441 ttccgaccgc caccgatgaa gtcctgggga ccaggaaatg gagtgccggg cccacagggg
     1501 tggctctcac catgcagggc ccctgggtcg ttggtgcgct tgcgaaccaa atttggtcct
     1561 atgcaggtaa cgatgatcgc gataaggtga gtgcgttcct gttccaatac ttcattaact
     1621 acaatttcaa agggggcctc tatctaacct ctgctcccat catcacggcc aattggaaag
     1681 ccaccagtgg caataaatgg gttatcccct ttggaggagg catagggaaa gtctttagga
     1741 ttggcaagat gcctctcaat gctcagactc aggcatttta caatgtgcag cagacgacgt
     1801 ttgggcccga ctggaccctt cgtcttcaag tgcaattcct ctttcccaaa taacccgtat
     1861 gatctcaagc aactcacctg accttctgct cattccaacc tcggcttcgc aaaacagatc
     1921 tcaaaacagg tttttatcaa ataaaatttc tgtaagccat tcactgtaaa agacttttct
     1981 ctcgcagacc cactgactgt tgagcagctg ctcttggcag acgcttccat aacttgagct
     2041 agggatggta tgaagattcc ggggaatgat agcgtgaatg tgtgaaagcc cgctcacgac
     2101 tgcggtttaa ccggtcgctg caacacatgg gttaaatcgt tcagcaggtg tttcaaagtt
     2161 taa
//
LOCUS       DAORAQ010000355         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7264_length_2155_cov_59.467143, whole genome shotgun sequence.
ACCESSION   DAORAQ010000355 DAORAQ010000000
VERSION     DAORAQ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7264_length_2155_cov_59.467143"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKM72_15195"
     CDS             complement(<1..664)
                     /locus_tag="PKM72_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNP62192.1"
                     /translation="MRSQWRTYFFTLAPKRWPKKRVLIVGDGVLAQALARVLVFDRSY
                     RYDVKGFLSNNPALVGTSLVNPMVLGTTEQLFEVVEKCEIETVAVCVEDRRGGLPLDS
                     LLDVKSMGLEVIDGHRLYETECGRLSIDELKPSFLIFSSGFRRKPIVMFLKRVGDVLG
                     AFLGLIALAPLFGLIALLVKSDSPGPIFYRQTRVGHHGYPYVLLKFRSMRNDAEAEGI
                     QWA"
     gene            complement(959..>2155)
                     /locus_tag="PKM72_15200"
     CDS             complement(959..>2155)
                     /locus_tag="PKM72_15200"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62193.1"
                     /translation="ERDYDNIQRHYQRLHENRINARISENLDKRQKGERFRILDPANL
                     PTKPEGKPRELIAVGGFIVGTGLGFGLAFLLDLLWPTFRRSEEAEISLGLATLATIPS
                     FKMAYGKSMKMVASELEAQNGSNGKHLALSQCVDSLLKGNGSDTEKEETESGASGHRH
                     KTFQEAFLPQLNLVTKWRPQSIVAEQYRVAATRLDLLDPGASNQVVLITSAMKGEGKT
                     STSANIAYTLARDLNEPTLIIDCDFKCPNLHRVLGLNQAPGVAEYFSGTNRLLEPCLQ
                     KIPDLPLWCMTVGDVGRYPVPLAKLQNLSSMLEMMTSRYRFIILDAPPVLPLADVNVL
                     SGLAQVVLMVVRSGVTPKDAVQKAAEMIHHAGPTRLVLTDAWTQGVPYYVRQSYIIPY
                     SLGSKG"
BASE COUNT          597 a          608 c          452 g          498 t
ORIGIN      
        1 tcgcccactg aattccttcg gcctccgcat catttcgcat cgaacgaaat ttcaacagga
       61 catagggata cccatgatgt cccactcgag tctggcgata aaagatcggt ccaggagaat
      121 ctgattttac caatagagcg attaaaccaa acaagggggc taaggcaatc agccccagaa
      181 acgcgccgag gacatccccc acacgcttaa gaaacataac gattggtttt cttctgaacc
      241 ctgacgaaaa aatgagaaag ctgggcttga gctcatcaat cgacaggcga ccacactcag
      301 tctcatacag gcggtgtcca tcaatcacct ccaggcccat ggattttaca tccagaagag
      361 aatccagggg caagcctccc cgacgatcct ctacacagac cgcaaccgtc tctatttcac
      421 atttttcaac gacttcaaac aactgctcgg tcgtcccaag aaccatggga ttgaccaatg
      481 aagttccgac caaggcagga ttattcgaca aaaacccttt gacgtcatac cggtaggatc
      541 gatcgaacac taagacacgc gcaagggctt gggccaaaac cccatcccct acaatgagaa
      601 cacgtttttt gggccaccgc tttggggcca atgtaaaaaa atacgtcctc cattgacttc
      661 gcataaaaag ccttagcaat ccgaggcgag tccacctcat cagcatcaca ttcaatatac
      721 tcggtcaggc cctggttgga tattggattg agcctccctg ctttgtctca cacccggtgg
      781 taggtattta agcagcctca tgccctagca catgcctcaa tgtctccaac aacagactgg
      841 aagacgacta agcaggcaat ccgaatgaag tcaaaataaa aatcacccgt cgcacaccgg
      901 atctctcaca ttcagcctac gacccatcgt ggaaatttta cttccccaca atctatctct
      961 accccttgct acccaaggaa taaggaataa tgtaactttg ccgcacatag taaggcacac
     1021 cttgcgtcca agcatcggtc aacacgagtc ttgtggggcc tgcatgatga atcatttcag
     1081 cagctttttg caccgcatcc tttggggtga ccccggaccg aacgaccatc aacacgactt
     1141 gagccaatcc gcttaagaca ttcacatccg ccaatggcaa cacgggtggg gcgtcgagaa
     1201 taataaaccg gtaacgtgat gtcatcattt ctaacataga agagagattt tgcaacttgg
     1261 caagcggaac aggataccga ccaacatccc ccaccgtcat gcaccacaac ggcagatcag
     1321 gaattttttg cagacagggt tccaacaacc gattagtccc ggaaaaatat tctgcaacac
     1381 ctggcgcctg atttaaacct agaaccctgt gcaaattcgg acatttaaag tcacaatcaa
     1441 tgatgagggt cggctcattc aaatcccttg ccagagtata ggcgatattc gcagatgtgc
     1501 tggtttttcc ctctcccttc atggcactcg taattaacac cacctggttg gaagcaccgg
     1561 gatccagaag atccagccga gtcgcggcca cccggtactg ctcagccaca atggattgag
     1621 gacgccattt ggtcactaaa ttcaactgag ggagaaacgc ctcctgaaag gttttgtggc
     1681 ggtgtccaga ggccccactt tcggtttcct ccttttccgt gtcagaacca ttccctttca
     1741 acagggaatc gacgcactga gacaatgcaa ggtgcttccc attggagcca ttctgagctt
     1801 ccaattcact tgccaccatc ttcatcgatt tcccgtaagc cattttaaaa ctgggtatgg
     1861 tggccagggt ggcaagccct aaagatattt cagcttcctc cgaacgtcgg aacgtcggcc
     1921 ataacaaatc caaaagaaag gcgagtccaa agcctaatcc ggtccctaca ataaacccac
     1981 ccaccgcaat taattcccga ggctttccct ctggcttggt cggcaaattg gccggatcta
     2041 aaatacgaaa tcgctctccc ttttgtcgtt tatctagatt ttcagaaatc ctggcattaa
     2101 tccgattttc atgcaggcgt tgatagtgcc tctggatatt gtcgtagtcc cgttc
//
LOCUS       DAORAQ010000356         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7433_length_2119_cov_68.132267, whole genome shotgun sequence.
ACCESSION   DAORAQ010000356 DAORAQ010000000
VERSION     DAORAQ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7433_length_2119_cov_68.132267"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..862)
                     /gene="purU"
                     /locus_tag="PKM72_15205"
     CDS             complement(5..862)
                     /gene="purU"
                     /locus_tag="PKM72_15205"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNP62194.1"
                     /translation="MSNAIAILLITCKDQKGLVARVAGFIHEFGGNILDSDHHTDRET
                     GDFLMRTEFDLAGFQIPHEEITPAFAPLAKIFNMNFQIYFTDRMPRIGILVSQYDHCL
                     VDVLQRHRRGELAIDIPVIVSNHQNSEWWADLFHIPFRYIPVTPQTKEQQEQEVLAAF
                     REQRVDLVVMARYMQIVSEKFLQEIGCPVMNIHHSFLPAFVGAKPYHQAYDRGVKIIG
                     ATAHYATPELDEGPIIEQDVVRVGHRDTVQDLIRKGRDLEVLVLARAIRLHVEHRVLV
                     YGRKTVVFD"
     gene            complement(1016..>2119)
                     /locus_tag="PKM72_15210"
     CDS             complement(1016..>2119)
                     /locus_tag="PKM72_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNP62195.1"
                     /translation="SLGVLLRYVLTEAGRQRATAFMELCRYVGPAPVPLNQYIDMIKS
                     QPVNSLTIDRRTVTAATEHLVLTPGTVDQLGEAVNSGWAIFLYGPPGNGKTVLAEAIG
                     KMLEAVGGGEVYIPYAMEVDGQIIQVFDPITHVRVEAPQEAARSLIEAKVEKDTRWVL
                     CKRPIEFAGGELTMATLDLSFNASAKYYKAPPHIKANGGVFLIDDFGRQLVRPRDLLN
                     RWIVPLEKRVDYLTLHTGKVFQIPFDTIVLFATNLEPAKLADEAFLRRIRNKIYIADP
                     TPERFTQIFEEVCRKKGVPYDPEAVQYLLDGVYVKYQLNMRSCHPRDLIEQIVSIAKF
                     NGVPPTLSKTFIDRACHTYFFVEAAEYDQNTSQ"
BASE COUNT          554 a          560 c          506 g          499 t
ORIGIN      
        1 ccccctaatc gaacacgacc gtttttcgtc catagaccaa cacccgatgc tcaacatgca
       61 gtcggatcgc tctggccagg accagcactt caagatcacg gcctttacga atgaggtctt
      121 gcacggtatc cctatgaccg acacggacca cgtcctgctc gatgataggg ccttcatcca
      181 attcgggtgt agcataatgc gcggtcgctc cgatgatctt gactccacga tcatacgctt
      241 gatgataggg cttggccccg acaaatgccg gcagaaagga gtgatgaata ttcataaccg
      301 ggcacccgat ttcctgcaaa aatttctcac tgactatttg catgtagcgg gccatcacaa
      361 ccaaatccac tcgttgctcc cgaaaggctg ccaaaacctc ctgttcctgc tgttcctttg
      421 tttggggggt aacaggaatg taccgaaagg gaatatgaaa gagatcagcc caccattccg
      481 aattctgatg attggaaaca atcaccggaa tatcgatcgc cagctcccct cgccgatgtc
      541 gttgcaacac atcaaccaga caatggtcat attgagagac cagaatacca attcgtggca
      601 ttcgatccgt aaaatatatt tgaaaattca tattgaaaat tttggcaagt ggagcgaagg
      661 cgggggttat ctcttcatga ggaatctgaa atcccgccag atcaaactca gtccgcatga
      721 gaaaatctcc ggtttcccga tccgtatggt gatcggaatc cagaatgttt ccgccaaact
      781 catggataaa cccagccacc ctcgcgacga ggcctttctg gtctttgcag gtaatcaata
      841 aaatagcaat ggcattagac atagggtttc cttcagggca atatccagga catagaggtc
      901 gggtagccaa cccacccggc aattgtgaat ggtggaggag agaaaaaacg attacccaac
      961 actccctccc tggagcattg ggcaatcata acggaaggca tcggtagggt aaacgttact
     1021 gactcgtgtt ttgatcgtat tcggcggctt ccacaaagaa ataggtatgg caggcacgat
     1081 cgataaacgt cttggagagt gtcgggggca ctccgttaaa cttcgcaata ctgacaatct
     1141 gctcgattaa atcccgggga tggcaacttc gcatatttaa ttgatacttg acatacacac
     1201 catccagtaa atactggaca gcctccggat catagggaac cccttttttc ctgcagactt
     1261 cttcaaaaat ctgtgtgaac cgttcgggag tcgggtcggc gatgtagatt ttatttctga
     1321 tccggcgaag gaacgcttca tcggccagct tggccggttc aagattggtg gcaaacaaga
     1381 caatggtatc aaacggaatt tgaaagacct tccccgtatg caatgtcagg taatccaccc
     1441 gcttttccaa tggcacaatc catcggttta acaaatctcg cggacggacc aattgtcgcc
     1501 cgaagtcgtc aatcagaaac actccgccat tggctttaat atgagggggc gctttgtaat
     1561 acttcgcgga agcattaaat gagagatcaa gcgtggccat cgtcaactcg ccgccggcaa
     1621 attcgatcgg acgtttgcag agcacccagc gagtatcttt ttccactttg gcttcaatga
     1681 gggatctggc cgcttcctgt ggagcctcga ctctgacgtg ggtgatggga tcgaacactt
     1741 gaatgatttg gccatccacc tccatggcat aggggatata cacttcgccc ccgcctaccg
     1801 cttccaacat tttcccaatg gcttcggcca ggaccgtttt cccgtttccg ggaggcccat
     1861 ataaaaagat ggcccagccg gaattgaccg cttccccgag ttgatcaacg gtgccaggag
     1921 tcaggaccag atgttccgtg gccgctgtca cagttcgtcg gtcaatggtc aagctattga
     1981 ccggctgact tttgatcata tcaatatact gattgagggg aacgggagca ggtccaacat
     2041 accggcacag ctccataaac gccgtggctc gctgacgacc cgcttccgtc aacacatacc
     2101 ggagtaacac cccaagcga
//
LOCUS       DAORAQ010000357         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7491_length_2108_cov_72.395519, whole genome shotgun sequence.
ACCESSION   DAORAQ010000357 DAORAQ010000000
VERSION     DAORAQ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7491_length_2108_cov_72.395519"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2108)
                     /locus_tag="PKM72_15215"
     CDS             complement(<1..>2108)
                     /locus_tag="PKM72_15215"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNP62196.1"
                     /translation="TFGLTDPPNILIVLLDDVGYGQMGTFGGAIPTPTLDRIANNGLR
                     YTRFHTTALCSPTRAALLTGRNHHSVGTGVIGEAGTGFPGYTGIIPASAATFAEVLRE
                     YGYANAWFGKNHNVPDWETSLVGPFDRWAGGLGFDYFYGFVGGDTDQFHPALVENMKR
                     IEPPDTNEDGSPYHLTTDLADHAIRMIRATKAVAPQRPFFVYFSTGATHAPHQVPQEW
                     IAKFKGQFDMGWDQYREQTFARQQRLGVVPAHAQLTPRPASLPAWDSLPEDERRVYAR
                     MMEVFAAFTAHTDHEVGRLIDAIENLGQLDNTLVIYMAGDNGSSAEGGLHGLLNEMTF
                     FNGLPESLEAKVASLDTLGGEKHYNHFPAAWAWAMDTPFQWTKQIASHFGGTRNGLAI
                     SWPQRIKARGEIRNQFHHVIDIAPTILEAVGVQAPAQFNGVAQKPIEGISMAYTFDNP
                     DIPDRRTTQYFEMLGNQGIYHDGWMASAIRGIPWLSENTPADLLDMSWELYHVEEDFS
                     QAVNLADKNPEKLNNLVKLFFAEAAKYNVLPLDDRKTERLNVANRPSLTEGRTSFTYP
                     NLLRLPEGASPNLKHKDHTITAKVVIPESKAEGMLFTQGGRFGGYGLYVQKERLIYHY
                     NLVGVARYTIMSKEPIPVGDVTLKAVYKSDTDKPFAGATVTLYANGNQIGEGRVEKSI
                     PNRVTLDETLDIGFDTGTPLT"
BASE COUNT          457 a          586 c          586 g          479 t
ORIGIN      
        1 gtcaacggcg tacccgtgtc aaaaccaatg tcgagcgttt cgtcaagagt cacgcggttc
       61 ggaatacttt tctccactcg gccttccccg atttggttcc cgttcgcata aagggtcacg
      121 gtggctccgg caaacggttt gtccgtgtcc gatttataca cggctttgag cgtcacgtct
      181 cctaccggaa tgggctcctt agacatgatg gtataccgtg cgacaccaac gaggttgtag
      241 tgatagatga gccgttcctt ctgcacatac agcccatacc ccccgaagcg acctccctga
      301 gtgaagagca tgccctcagc cttactctca gggattacca ccttggcggt aatcgtatgg
      361 tccttgtgct tgagattagg ggaggccccc tcaggaagcc gaagcaagtt aggataggtg
      421 aaagaggtac gaccttctgt gaggcttggg cggtttgcca cattcaaccg ctcggttttc
      481 cgatcatcga gaggcaacac gttatatttc gcagcttcgg caaagaagag tttgacgagg
      541 ttgttgagct tctccggatt cttatctgca agattcaccg cttggctgaa gtcctcctcc
      601 acatggtaga gctcccagga catgtccagc aggtcggcag gggtgttctc gctgagccag
      661 ggtatcccac gtattgcact ggccatccat ccgtcatggt aaatgccttg gttcccgagc
      721 atctcgaaat actgggtagt tcgccgatcg ggaatatccg gattatcaaa ggtgtaggcc
      781 atgctgatcc cctcaatggg cttttgggca actccattga attgagccgg ggcttgcacg
      841 ccaacggctt ccagaatagt cggggcgata tcgatgacat ggtggaactg atttcggatt
      901 tcccctcgcg ccttgatgcg ctgcggccat gaaatggcga ggccattgcg cgttccacca
      961 aaatggctgg caatctgctt cgtccactgg aacggcgtat ccatggccca agcccaagcc
     1021 gcgggaaaat gattgtaatg tttctctccc cctagggtgt cgagggaagc gaccttagcc
     1081 tccaacgatt cgggaagccc gttaaagaac gtcatttcat tgagcagccc atgtaagcct
     1141 ccttcggcac tcgacccgtt gtccccggcc atgtagatca cgagggtatt atccagttgc
     1201 cccaaattct cgatcgcatc gatgagccgt cctacctcat ggtcggtatg agcggtgaag
     1261 gcggcaaaga cctccatcat cctggcatac accctccgtt catcttcagg caatgaatcc
     1321 caggctggta acgaggcagg gcgcggggtg agttgtgcgt gtgcaggaac aaccccgagg
     1381 cgctgttgtc gcgcaaaggt ctgctctcga tattggtccc accccatgtc aaactgtccc
     1441 ttgaatttgg cgatccattc ctgtggcacc tggtgcggag catgggtggc accggtggag
     1501 aagtacacaa agaacggtcg ttggggggcc acggctttag tcgcccggat catccggatg
     1561 gcgtggtcag ccagatctgt cgtcaagtga tacggcgagc catcttcatt ggtatcaggg
     1621 ggttcaatac gtttcatatt ttcgaccaag gccggatgaa actggtcggt atcaccaccg
     1681 acgaagccgt aaaagtaatc aaaccccaat ccacccgccc aacgatcgaa gggccccaca
     1741 aggctggtct cccagtctgg aacattgtgg tttttgccaa accaggcatt ggcgtatccg
     1801 tactcccgaa gcacctccgc aaatgtcgcg gccgatgctg ggataatgcc ggtataccca
     1861 gggaagccag ttccggcttc gccaatgacc cccgtgccca cggagtggtg gttacgccct
     1921 gtcaggagtg ccgcccgagt tgggctgcag agcgccgtgg tgtggaaccg ggtgtaacgc
     1981 aacccattat tggcaatgcg gtctaaggtg ggagtcggaa tggcgccacc gaaggtgccc
     2041 atctgcccat aacccacgtc atcaagcaga acaatgagaa tattgggtgg atcggtcaag
     2101 ccaaacgt
//
LOCUS       DAORAQ010000358         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7611_length_2086_cov_65.191531, whole genome shotgun sequence.
ACCESSION   DAORAQ010000358 DAORAQ010000000
VERSION     DAORAQ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_2086_cov_65.191531"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKM72_15220"
     CDS             complement(<1..574)
                     /locus_tag="PKM72_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62197.1"
                     /translation="MCISDSLRTRSGVPIALRKSQSPLKILCVLLLIMSLSGCSRQHV
                     GATDHVPKTESAIIVVPGYYGTRLVREADRSVVFISLTQALFGRQSLTLPVPELGFKG
                     TIDLQPDGILDEVRVVPLLYSIDVYGSLLDRLRVSNQRSGEVIPFTYDWRGDLMEAVR
                     SLDVLIRRLRGEGKKDISIVAHSMGGLIVSY"
     gene            complement(990..1157)
                     /locus_tag="PKM72_15225"
     CDS             complement(990..1157)
                     /locus_tag="PKM72_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62198.1"
                     /translation="MPKAKREKNCYVCENAELIRMSFMTCRQCARHFCSRHGDPKMDE
                     CTNCLDGDEET"
BASE COUNT          546 a          536 c          518 g          486 t
ORIGIN      
        1 agtagctgac aatcaggcct cccatactgt gcgcgacaat agagatatct ttctttccct
       61 cgccccggag ccggcgtata agaacatcca aacttcgaac agcttccatg agatccccac
      121 gccaatcata ggtaaagggg atcacttccc cactcctctg gttggagacc cggagtcggt
      181 ccagcaagga gccatagacg tcaatggagt acaaaagcgg gacgactcga acctcatcaa
      241 gaatgccatc gggttgcaaa tcgattgtcc ccttaaatcc caattcagga acgggaagtg
      301 ttaacgattg acgaccgaac aacgcctggg tgagggaaat gaaaaccaca ctgcggtccg
      361 cttcgcggac taaacgcgtg ccgtaatacc ccggcaccac aataatcgcc gattcggttt
      421 tgggaacatg atccgtcgcg cccacatgct gtcgactgca cccggacagc gacatgatca
      481 gcagcagcac acacagaatt ttcagggggg attgtgattt ccgaagggca atcggaacac
      541 cactccgggt gcgtaacgaa tcagagatac acatagaatg ttctgaccgt cgttgcccta
      601 agcctgcaga ttaagaccca tggctatacc caacacgtgt ggcggacttg atagaagtcc
      661 taccccattc tctccttcta aggagttaga gcagattggg tcggagaata gcgtctcaat
      721 gcccgcttag tgtataaaat tgagaggatg tgtggaaaga cgattgacct cagatgtggg
      781 gtgaatgttc gcgatctcaa tgggattgag accagacatc cgaacacgaa tcagtccgga
      841 tgattgccat tcccaagccg cgcactcctg cccctttcat tcgctgctca tcgttcggat
      901 tgcaccataa ggtgcaatcc cggtttctgg atggcccgag tcaatgctcc ctgctggaga
      961 aggcccgacc caactgttgg tgagccggtc taagtttcct catcgccatc aagacaattg
     1021 gtacattcat ccattttggg gtcaccatga cgactacaaa aatgacgagc gcattgccgg
     1081 caagtcataa aactcattcg aattaactcg gcattctcac acacataaca attcttttct
     1141 cgtttcgctt ttggcatcgt tcttctcctt aggtagccat cttctcaagt catttcaaga
     1201 cataggacgc acaggcaaag ccccgagggc acagatatga cgccaacgat ttcagtctgt
     1261 catagccggg agaggcgtga gaccatcatc atgcgattgg cgtgaacgtc agggagggcg
     1321 tgcatctcgc accgacgcgg ggagcggacg ctgatccgta acaagggaaa gggagcctac
     1381 cgcatgaccg aacctcctgg caatgggaag ccaatctttg tcacctctac cccgggaacg
     1441 ggatgtaggg ctcggaataa tttccggggg gtctagcctc gctcagtgga tgggtttaca
     1501 atccatatta ttctcaggtc aaaagacacc tcagccattc ttgggatatc gatagggaat
     1561 ccgaccgtgt aaatcgctca atttttaagg cagcgcgtca tttttcgatt gcaaattgtt
     1621 gacattttgg gtatgtctat ccaccctaaa caagtgcttg attgaaaagt ttctatcttg
     1681 ttcacagaca tgagcgaaag gcaaaagcag aaagtgatac agatcgggta attcccctac
     1741 ggtggccttg aagagtatgg ccacatgccg gcagagaatg taacgctgac ccatagtttc
     1801 cgggtccatt ccatcaacag ttggatccct gggagaggga agaccgcttc gctttccagg
     1861 ttcatttcac aatcatcgat tgaaattttc tcaatggttc acagagggat ggggaaccag
     1921 aaaccttccg gtacacctgt gccacttttg tatcggtcgc atgttcccaa ataagggagt
     1981 gttgaataat aatttccaag agcatattga cccacaggca cgttgcatat tagaggcctc
     2041 ggggcggttc aaaaaagcat gtcctgaggc tttcgaaggg ttcgtc
//
LOCUS       DAORAQ010000359         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7621_length_2085_cov_68.557143, whole genome shotgun sequence.
ACCESSION   DAORAQ010000359 DAORAQ010000000
VERSION     DAORAQ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_2085_cov_68.557143"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKM72_15230"
     CDS             <1..683
                     /locus_tag="PKM72_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNP62199.1"
                     /translation="KSGESEELLALLPFIRKMGARIIAITAQVNSTLARGSDLVLVTP
                     IQEEACPLNMAPTCSTTAALVLGDALAMALMKLRNFQPADFAMFHPGGQLGKRLLLNV
                     GDLMRTGEGNAVIRLSHTIQFMLCEMTSKRAGAVSVLDEEDRLLGLITDFDIRRVLEG
                     GQNIFALTIATVMNPKPSWVYQDEKAVKVLQFMENREKPISVLPVLDRQEKVVGMIHI
                     HDLISRGL"
     gene            917..>2085
                     /locus_tag="PKM72_15235"
     CDS             917..>2085
                     /locus_tag="PKM72_15235"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNP62200.1"
                     /translation="MSIHLDVGQVGNNPSSKVFSSSDLWAALGWALCAVILLGFSPRL
                     FHIQEYFFFPLLGMAILLCLWERKPFWIHASLLIPFICFVSWVAFTIPFSIDPMISLR
                     EWQKVVAQFAVFYGTCLIVQEQRNEYFLKKVLPVVLVGAIACYTYSLLDFFDRGGNLL
                     DRTIRAGFPKIDGADFTWLSTNVLMVFPLLITGFLAVTNPWKRVMLVCGILLSFLALL
                     LSYSRGVWLAALAQIIVGGYLVHKRHTLRLLIIGIAVFLSIGVVLSQLGLHRETFNSW
                     TIKARLAVWNLSVSDVLNHPIVGVGYGVPILEKRHGQNIVDLEAVNLGIPDIPEKPHN
                     WYLMVVTGTGLPGLVLFLWLLAKVGLAIYEKWTKAMTSAHRWLQMGVFLMVLGFS"
BASE COUNT          480 a          431 c          520 g          654 t
ORIGIN      
        1 gaaagtcagg cgaaagcgaa gaattgctag cactgcttcc atttattcga aaaatgggcg
       61 cacgaattat tgccattacc gcgcaggtaa attccaccct ggcacgcggg agtgatcttg
      121 ttcttgtgac gcccattcaa gaagaagctt gtccgcttaa tatggctcct acgtgcagca
      181 ccactgcagc cttggtctta ggggatgcgt tggccatggc cttgatgaaa ttgcgaaact
      241 ttcagccagc cgactttgct atgtttcacc ctggtggaca attgggaaaa cgactgcttt
      301 tgaacgttgg ggatctgatg cgaacaggtg agggaaatgc ggtaattcgt ctttctcata
      361 cgattcaatt catgttgtgt gaaatgacga gtaaacgtgc tggcgcagtg tctgtgctgg
      421 atgaggaaga tcgtttattg ggattgatta ctgactttga tattcgtcgg gtgctggaag
      481 gggggcaaaa catttttgct ctcacgattg caacggtcat gaatccaaag cccagttggg
      541 tatatcagga tgaaaaagcc gtcaaggttc ttcaatttat ggagaaccgg gaaaagccca
      601 tctccgtgtt acccgtcctt gatcgacagg aaaaagtcgt cggcatgatc catattcatg
      661 acctgatttc ccgtggtcta tagcaaatat tcgtgttcaa tgcgaagagt cttggatttg
      721 attcaagagg tgcgtcgtag tagggggaca gcgtgatggt tagcatccat atcgattcct
      781 taaattcggg tagccggttt gtctgcgtat ggggcaacag aatttcaaat ttttttgccc
      841 accgctgaat ggctattgag gattggttca aaagcgcacc ttctgccaga ctgcttgtct
      901 caaatgaatt gaaacaatga gcatccactt agacgtggga caagtcggta acaacccctc
      961 ttcaaaggtt ttctccagct ccgatctttg ggctgccctg gggtgggccc tttgtgcggt
     1021 cattctttta ggattctctc ccagactatt tcatattcaa gaatatttct ttttccctct
     1081 tctgggtatg gcaatcctcc tttgtttatg ggagcgaaag cctttctgga ttcatgcctc
     1141 actccttatc ccgtttatct gttttgtatc ctgggttgcg tttacgattc ctttttcaat
     1201 tgatcctatg atcagtctac gggaatggca aaaagttgtg gcacagtttg cggttttcta
     1261 tggcacctgt ttgattgtgc aggaacaacg gaatgaatat tttctaaaaa aagttctccc
     1321 cgttgtcctt gttggagcca ttgcttgtta cacctattcc cttctggatt tttttgaccg
     1381 aggagggaat cttttagatc gcacaattcg tgcgggtttt ccaaaaatag acggtgcaga
     1441 ctttacctgg ttgagtacca atgtgttgat ggtttttcct ctgcttatca cgggattttt
     1501 ggcggtgact aatccatgga aacgggtgat gttggtctgt ggcatacttc tctctttctt
     1561 ggccctcctg ctttcctata gtcgtggtgt ctggttagca gccctggcgc aaataattgt
     1621 cggaggatat ctagttcata aacggcatac gctccgattg ctgatcatag gaatagcggt
     1681 attcctttca attggagtcg tccttagtca attggggcta catcgtgaaa ccttcaattc
     1741 ctggactatt aaggctcggt tagcggtatg gaatcttagt gtttcagatg ttttgaacca
     1801 tcccattgtt ggagtcggtt atggcgtacc tattttagaa aaacgtcatg ggcaaaatat
     1861 tgttgacttg gaagcagtga atctcggaat tcctgatatt ccggaaaaac cgcataactg
     1921 gtatctcatg gtggtcacgg ggacgggatt gccagggctg gtcttgtttc tttggttact
     1981 cgccaaagtg ggattagcta tttatgagaa atggactaaa gctatgactt ccgcacatcg
     2041 gtggttacag atgggagtat ttctgatggt tcttggattt tccat
//
LOCUS       DAORAQ010000360         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7799_length_2051_cov_67.097695, whole genome shotgun sequence.
ACCESSION   DAORAQ010000360 DAORAQ010000000
VERSION     DAORAQ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7799_length_2051_cov_67.097695"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..1287
                     /locus_tag="PKM72_15240"
     CDS             433..1287
                     /locus_tag="PKM72_15240"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNP62201.1"
                     /translation="MDINAQTQLCGLLGNPVDHSLSPAIHNAAFRELGLNFVYLAFPV
                     QDLERAVLGIKALGNIRGLSVTIPHKVTILPLLDTVETTAKHIGSVNTIIKDRGLLVG
                     SNTDASGALQALQQGGVERTGQRVVILGSGGAARAIAFGLCVEGKIEHLTLLGVDDQE
                     RTTLARDLKAKTPIHLHDASLTPETLHSALGQAQLLIHCTPIGMHPKIDESCVPKHLL
                     HRDLTVMDIVYNPLNTRLLQEAQAAGCRTIQGINMFLYQAVGQFELWTGKSAPTEVMR
                     NVLTSHFT"
     gene            1535..2041
                     /locus_tag="PKM72_15245"
     CDS             1535..2041
                     /locus_tag="PKM72_15245"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNP62202.1"
                     /translation="MNIVLIGYRGTGKSTVATILGQRLGRTVISTDEEIVKEAKQSIP
                     QLIEQFGWDHFRNLETGVCQKLTGQENLVIDTGGGLILKEENVRMLKENGKIFWLTAE
                     VSTIASRISGDTQRPSLSGTKSFVEEIEEILEIRTPHYQAAADHVIQTDQISPEHIAD
                     AILSHISI"
BASE COUNT          561 a          559 c          451 g          480 t
ORIGIN      
        1 agaccattcc ctcaaacctc tttcttctaa tcgaccggtc accggtcagc aaaaaagtcc
       61 ttccaatccc tcaggatttt tatcgtccta ccgataaacg tggatagtat tgcttacgac
      121 attattggaa gggaggtgga accatgaaaa aagttgcaaa aaagaaaccg gcaaaaaaag
      181 ccgcggtgcg aatgaaaaag tctccggcaa aaaaaaaggc gtcacgataa cgcccccgga
      241 catcacactg tgtagatccg gggagtcgaa tctactcccc ggattacacc ctcccttctc
      301 tcaaccctaa tttcacttcc tcattcatct cgacctccca gacaacgttc gcattcacac
      361 aagttgagtt tccagccagg ttatgtgata actctcgatc actatttctc atctatttcg
      421 agagcacctg ttatggacat taatgcacaa acccaactct gcggcctgtt aggcaatcct
      481 gtcgatcact ccctttctcc cgctattcat aatgcggcct ttcgtgaatt gggattaaat
      541 tttgtctatc tggcctttcc cgtccaggat cttgaacgag ccgttctagg catcaaggca
      601 ttaggcaata tccggggact cagcgtgacc attccgcata aggtcaccat tctacctttg
      661 ttggataccg tcgaaaccac cgcgaaacac attggctcgg tgaataccat catcaaagac
      721 cgtggcctgt tagtgggctc caacactgac gcatcgggcg ccctccaagc cttacagcaa
      781 ggtggagtgg aacgcaccgg acaacgggtc gtcattctag gatccggtgg agccgctcgc
      841 gccattgcct tcggtctctg cgtggaaggc aaaattgaac atctcaccct ccttggtgtt
      901 gatgatcagg aacggacaac cttggcaaga gacctcaaag ccaaaactcc catccacttg
      961 catgacgcat ccctaacacc cgaaaccttg cattccgcgt tgggacaagc ccaactactc
     1021 attcattgca cccccattgg catgcaccca aagattgatg aaagctgtgt ccccaagcat
     1081 ctgttacacc gcgatctgac ggtgatggat attgtctaca acccactcaa tacgcgttta
     1141 ttacaagagg cgcaggcggc aggatgccgc accatacaag ggatcaacat gtttctctat
     1201 caggctgtcg ggcagtttga actttggact ggcaaatccg caccgaccga ggtcatgcgt
     1261 aacgtgctca cctcccactt cacttgaata aagcatatcg agtttggata tggttcccag
     1321 cgtcaacgag agacttttcc agggagattt caataggaga aagaatggcc gtggcgacat
     1381 tctcccaaag atgtcccctg cccgaaggcc ggaacaaaaa gttccctggc gtcctctccg
     1441 ctattcccaa cggctttaaa gagaaattac tttcccgtta cgggccaacc tgtccgaaca
     1501 tgtttttcaa ccaacacttc aaaacaggag atgtatgaat attgtcttaa taggatacag
     1561 aggaacggga aaaagcactg tggcaacaat tttgggacaa cgtctcggac gaacggtcat
     1621 ctccactgat gaggaaatcg tcaaggaggc gaagcaatcc attccacagc ttatcgagca
     1681 attcgggtgg gatcatttcc gcaatcttga aacgggagta tgtcaaaagc ttaccggtca
     1741 ggagaatctg gtcatcgaca cgggcggggg actcatcctt aaggaagaaa acgtcagaat
     1801 gctgaaagaa aatggaaaga ttttctggtt gactgccgaa gtttcaacga ttgccagccg
     1861 gatttccggc gatacgcagc ggccctcgct gtcaggaact aagtccttcg tggaagaaat
     1921 agaagaaatt cttgaaattc gcacacccca ttaccaagcc gccgccgacc atgtgattca
     1981 aaccgaccaa atttctccgg aacacattgc tgatgccatt ctttcccaca tttcgatcta
     2041 gaacgttcac t
//
LOCUS       DAORAQ010000361         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7803_length_2050_cov_63.650627, whole genome shotgun sequence.
ACCESSION   DAORAQ010000361 DAORAQ010000000
VERSION     DAORAQ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7803_length_2050_cov_63.650627"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..120)
                     /locus_tag="PKM72_15250"
     tRNA            complement(47..120)
                     /locus_tag="PKM72_15250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Gln,seq:ttg)
     gene            complement(174..869)
                     /locus_tag="PKM72_15255"
     CDS             complement(174..869)
                     /locus_tag="PKM72_15255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP62203.1"
                     /translation="MGRNVAEKEGEMMERVLEPEIMDEPEQVQAYAEADFQEENQGFV
                     DNFLRLYEDRDGPHVVDVGCGPGDISIRLARSHPTCRITGIDASIPMITWAEQAVKQA
                     GLAHRIEFLSQRFQDVSLATPADAVMSNSLAHHVPNPLRFWYEIKKMLKPGGLVLVMD
                     LLRPDSPEEAQALVDQYAAQEPKRLQQDFFHSLLAAFTEDEVAAHLAELNLSRLMVDV
                     PDDRHWIVYGRVY"
     gene            complement(833..1378)
                     /locus_tag="PKM72_15260"
     CDS             complement(833..1378)
                     /locus_tag="PKM72_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNP62204.1"
                     /translation="MATILIVLDHYIAELNKFMGSVPYLYQINCSPGGLPKLPVSKAW
                     ITFNGINGDRSRNRVLHGGPDRALCLFSFEVLKALQREGHTVHAGASGENLTVAGLNW
                     AQLKPGDHLKIGEEVQIELTSYCEPCRHNAQWFVNGDFFRISHRQHPGWSRLYARVLS
                     EGRVRQGDGVWVETLPKRKVK"
     gene            complement(1413..>2050)
                     /locus_tag="PKM72_15265"
     CDS             complement(1413..>2050)
                     /locus_tag="PKM72_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNP62205.1"
                     /translation="GPTKPVLLWCHGNAGNISHRLDNIRELYRWGLSVFIFDYRGYGR
                     STGTPSESGLYQDALAGYDYLLNRRGIDSNRIVLFGRSLGACVAGDVAIRRASAGIIL
                     EGAFPSIQAMSDHHYLGLPARWLLNVDFNLAEKVAKLRRPLLVIHGEKDSIVPVSLGR
                     QVYDAAHEPKQWSVVVGAEHNDVPFVGGTAYFQKIMGFVQTVVSKGHGGSQ"
BASE COUNT          544 a          567 c          473 g          466 t
ORIGIN      
        1 ggaatagagt ttgtcgtaga tatgttgatg ggaggaggat attgactggc tgggggacca
       61 ggaatcgaac ctgggttcta agatccaaaa tcttatgtct tgcccctaga cgatccccca
      121 accgaacgag cactttaaga cttacaggac caaagcgtca agatctttac cggttagtac
      181 acccttccat agacgatcca atgccgatca tccgggacat ctaccatcaa acgacttaaa
      241 ttaagttctg cgaggtgggc agccacctca tcctccgtaa atgccgccaa cagggagtga
      301 aagaaatcct gctgtaatct ctttggttct tgagccgcat actgatcgac gagcgcttga
      361 gcctcctcgg gagaatccgg acgaaggaga tccatgacca gcactaaccc gccgggtttg
      421 agcatttttt ttatctcata ccaaaatcgt aggggattgg gaacatggtg ggccaaactg
      481 tttgacatta ctgcatcagc tggagtggcc aaactgacat cttgaaaccg ttgagagaga
      541 aattctattc gatgagctaa cccagcttgc ttcacggcct gttccgccca ggtaatcatg
      601 gggatggagg catcaattcc tgtaatcctg caggtgggat gactccttgc caggcggatg
      661 gagatatcac cggggccgca acccacatcc acaacatgag ggccatcacg atcctcatag
      721 agtcgaagaa aattatccac aaaaccttga ttttcttctt gaaaatccgc ctcggcatag
      781 gcctgaactt gctcaggttc atccataatt tccggttcga gcacccgttc catcatttca
      841 ccttcctttt cggcaacgtt tcgacccaca cgccatcacc ttgccgtacg cgtccctcgg
      901 acaacactcg agcatataac cggctccatc caggatgctg ccggtgagag atccggaaaa
      961 agtctccatt cacaaaccat tgcgcattat gacggcatgg ttcgcaatag ctagtcaatt
     1021 cgatctgcac ctcttctcca attttcaaat ggtcccctgg cttcagttgt gcccagttca
     1081 gaccggccac ggtcaggttt tctccggagg ctccagcgtg aacagtgtgt ccctcgcgtt
     1141 gtaaggcctt aaggacctca aacgaaaaca ggcataatgc ccgatccggc ccgccatgta
     1201 gtacccgatt ccgagaccgg tctccattta tcccattgaa ggtgatccag gccttggaca
     1261 cgggcagctt ggggagtccg cctggagaac aatttatttg atagagatac ggaacagaac
     1321 ccatgaactt gtttaattcg gcaatatagt ggtccagaac aatgaggatg gtagccatca
     1381 atcaaaatga tggtcaagca ggattacatc attcattgcg atcctccatg tcctttggag
     1441 accacagtct gaacaaagcc catgattttc tgaaaatagg cggttccccc aacaaacggc
     1501 acatcattat gttccgcacc cactaccaca gaccattgct taggttcatg tgcggcatca
     1561 tagacctgcc gccccaagga caccggaaca atggaatcct tttccccatg aatcaccaat
     1621 agcggacgcc tgagcttggc aaccttttca gccagattga agtccacatt tagcaaccac
     1681 cgagcgggca atcctaagta atggtgatcc gacatggcct ggatagatgg aaacgcccct
     1741 tcaagaatga ttccggccga tgccctgcga atagccacat ccccagctac gcaagcgccc
     1801 agagaccgtc caaacaggac aatgcggttc gagtcaatcc cgcgacggtt caaaagatag
     1861 tcatatccgg ccaaggcatc ttgatacagg ccggattcag aaggagtgcc cgtgctccgg
     1921 ccatagcccc gataatcaaa aatgaaaaca gataatcccc accgatacag ctcacgaata
     1981 ttgtctaacc gatggctgat attgcccgca ttgccatgac accacaggag caccggcttg
     2041 gttggtccag
//
LOCUS       DAORAQ010000362         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_7947_length_2025_cov_67.375127, whole genome shotgun sequence.
ACCESSION   DAORAQ010000362 DAORAQ010000000
VERSION     DAORAQ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_2025_cov_67.375127"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKM72_15270"
     CDS             complement(<1..682)
                     /locus_tag="PKM72_15270"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNP62206.1"
                     /translation="MARVLQQVPQPRTSLDIRIVTYNIHRCCGMDRRIMPRRIAEVLS
                     ALHPDVIALQEVLGLGPKGRGQDELLGALLGMGWVMASARQRHGYPFGNMILSRFPIV
                     DDARHDLSWRTRRGRCCQRADLRLGNQTLHVYNVHMGTGMRERKYQAERLAMILDQHQ
                     ATGPKIILGDFNEWGRGVTTDILTPRFQSLDIRPFVKRRRTYPGFLPLLHLDHIFYEG
                     QIDVTHVHL"
     gene            complement(1181..1426)
                     /locus_tag="PKM72_15275"
     CDS             complement(1181..1426)
                     /locus_tag="PKM72_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62207.1"
                     /translation="MPLVLGGGIGERAPDIRTRMGEGIGWCGPSWDSPRNFEGSDVQA
                     GEAIPIHRKESAIQFPVIGTDKESWVASETCQYLKNP"
     gene            complement(1395..1598)
                     /locus_tag="PKM72_15280"
     CDS             complement(1395..1598)
                     /locus_tag="PKM72_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62208.1"
                     /translation="MNSRSGLLNLSSLTSDLPHLLQALREQPDPRATLAIDVFCQRAR
                     KYLGAYLAILGGTDAISFGGRHW"
     gene            complement(1775..>2025)
                     /locus_tag="PKM72_15285"
     CDS             complement(1775..>2025)
                     /locus_tag="PKM72_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62209.1"
                     /translation="LCLLLLWRHRADDINMSSVWLCSRNDIIANVSVLCAAAGVWLTS
                     SGWPDILVGFGLAALFLRSSLYVLRGAHTELRAVSPSN"
BASE COUNT          529 a          485 c          527 g          484 t
ORIGIN      
        1 gaagatgcac atgagtcacg tcaatctggc cttcataaaa aatatggtcg agatgtaaca
       61 ggggcaaaaa tcccggatag gtccggcgtc gtttcacaaa ggggcgaatg tccaggctct
      121 gaaatcgtgg ggtgaggata tcggtggtga cgccgcgccc ccattcgtta aaatcgccga
      181 gaatgatttt cggaccggtt gcttgatgct ggtccagaat catagccagg cgttccgcct
      241 gatacttgcg ttcccgcatt cctgtgccca tatgcacgtt gtagacatgt aaggtctgat
      301 tacccagccg gaggtcagcg cgttgacaac aacggccgcg ccgggttctc caggacaggt
      361 catgtcgggc atcatctact ataggaaatc ggctcagaat catatttccg aagggatacc
      421 cgtgacgttg ccgggcagat gccattaccc atcccatacc caacaatgca ccaaggagtt
      481 cgtcctgacc acggcctttg ggtccaagtc ccaggacttc ttgcagagca ataacatccg
      541 gatgcagggc cgataggact tccgcgatcc gtctgggcat gatccgacga tccatcccgc
      601 aacaccggtg aatgttatag gtcacgattc gaatatcgag ggaagtccgt ggttgaggaa
      661 cctgttgaag tactctggcc atgagcgaat agaaagtcct tttaaatcac tgagctatca
      721 ttgctctata gaccatatca tgacgggaaa agatgtgagg cataatacaa aatggctcat
      781 tatcatgatt ggcaaaatgc agaccagaat agaaaaagac cgtccacaaa ttttgtggat
      841 aacctggtgg ctaccggcaa caaaagcggt atttttgaag gattgataga gaaatgagca
      901 aattgcgtaa attttaggca ttgtgttgat aatatttttc aatactaaat atagtggtgt
      961 gaattttttg gcgtagtcgt aaaggtagaa tgatgatttt atcttgactt ttttgattca
     1021 gttgatcctt ttgagtttca tcaaaagctc ataatgattt cctgaaaatg gtttatccac
     1081 agaaaggttt ttcccttgtt gatagtgaac aaaatgagaa tggttttgtc gacgcctgta
     1141 gggtcttttc gccttggcaa taaattttgg cctgtgttca tcatggattt ttcagatatt
     1201 gacacgtttc gctcgctacc cacgattctt tgtcggttcc gatcaccgga aattgaatag
     1261 cggattcctt gcgatgaatg ggaatggctt ctcctgcctg cacatcggag ccctcgaaat
     1321 ttcgagggct atcccacgag ggaccgcacc aaccgattcc ttcgcccatt cgagtacgga
     1381 tgtcgggagc ccgctcacca atgccgcccc ccaaaactaa tggcatcggt gccacccaag
     1441 atggccagat aggctcccag gtattttcgc gcgcgctgac agaacacatc aatggcgaga
     1501 gtggcccgag gatccggttg ctcgcggagg gcttggagaa ggtggggcaa gtcggaggtc
     1561 aggctggaca agttcaacag gccggatcgg gaattcaact gagggtcgat cttttcaagg
     1621 gaaagccgat tatgcccggc gacctatccg ataatggtca ggtcgggatt tccacgtgtg
     1681 cccatcacga ggccttctag ccggtgaatc ccattgaggc ctcgcccaca gctctatttt
     1741 tgatggcgct aactgagcac cattttccta aaggttaatt tgaaggagag acggcgcgaa
     1801 gctccgtatg agccccgcgc aagacatata gggaagaccg gagaaaaaga gccgcgagtc
     1861 caaaacccac gagaatgtca ggccagcctg aactggtaag ccatacccct gcggcggcac
     1921 acagcaccga gacattcgca ataatgtcat tgcgggaaca tagccacacg gaactcatat
     1981 tgatgtcatc ggcccgatgg cgccagagca atagaaggca aaggc
//
LOCUS       DAORAQ010000363         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8084_length_2004_cov_70.630067, whole genome shotgun sequence.
ACCESSION   DAORAQ010000363 DAORAQ010000000
VERSION     DAORAQ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_2004_cov_70.630067"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /gene="dnaE"
                     /locus_tag="PKM72_15290"
     CDS             complement(<1..1189)
                     /gene="dnaE"
                     /locus_tag="PKM72_15290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNP62210.1"
                     /translation="MPAQFVHLHLHTQYSLLDGANQLTPLFQQVKNFGMPAVAMTDHG
                     NLFGAIDFYQKAKAHDVKPIIGCEAYMAPGNRTQRAGHLAHNEYFHLILLATNQVGYH
                     NLIKLSSKAYLEGFYYKPRMDKELLQEHQEGLIALSGCLSGEVPQLINHQDMEGALRV
                     ADEYRSIFGKDRYYLEVQANGLDHQLVANRGLLEIHKKLGIPLVGTNDCHYLNKRDAR
                     PHEIMLCLQTGKTLKDPNRMKFDTDQLYVKSTEEMIAEFSEFDRAVLNTTQVAEQCDL
                     QLEFGTTYLPDYHVPEGQSHDSYLQHLSEEGLRARLRERPTAIAPEVYQQRLHTELAV
                     LNAMGYAGYFLVVWDIINFARSRKIPVGPGRGSAAGSLVAYALRITDLDPLTYNLLFE
                     RFLN"
     gene            1803..>2004
                     /locus_tag="PKM72_15295"
     CDS             1803..>2004
                     /locus_tag="PKM72_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNP62211.1"
                     /translation="MILTTTHNVEGHQIKDYLGVVAGEAILGTNFFKDFFANIRDIVG
                     GRSGAYEKELRRAREIAFQEIQE"
BASE COUNT          539 a          415 c          534 g          516 t
ORIGIN      
        1 ggttgagaaa gcgttcaaag agcaggttgt aggtgagggg atcgaggtcc gtaatgcgca
       61 gagcgtaagc cacgaggctg ccggcggccg atcctcgtcc tggcccgact ggaatttttc
      121 gggatctggc gaaattgatg atatcccaca caaccaaaaa gtatccggca taccccatgg
      181 cattcagcac ggcgagttcg gtgtggagtc gctgctggta gacctcgggg gctatggcag
      241 tcgggcgttc ccgtaaacgg gctcgaaggc cttcttctga aagatgctga aggtaggagt
      301 catgtgattg accctcaggt acgtgataat caggcagata tgtggttccg aattccaatt
      361 gaagatcgca ctgctcggcc acctgggttg tattcaagac tgcacggtca aattctgaaa
      421 attccgcaat catttcttct gtggacttca catataactg gtcggtgtca aacttcatcc
      481 ggttaggatc tttgagcgtt tttcccgttt gaagacacag catgatttca tgcggacgcg
      541 catctcgttt attgagataa tgacaatcgt tggtgcccac cagcgggatg ccaagcttct
      601 tatgaatctc cagtaatccc cgattcgcaa caagttgatg atccaggccg ttggcctgca
      661 cttccaggta atagcggtct tttccaaaga tggaccgata ctcatcggcc acccggagag
      721 ccccttccat atcctgatgg ttaatgagct gaggcacctc accgctcaaa caccctgaaa
      781 gggcaatgag accttcttga tgttcctgta ataattcctt gtccatgcga ggcttataat
      841 aaaagccttc aagataggct ttgctggaga gtttaatcag gttgtgatag ccaacttgat
      901 tggtcgcaag aagaatcaga tgaaaatact cgttatgcgc aaggtggcct gcccgctggg
      961 tacggttgcc tggagccata taggcttcac acccgataat cggtttcaca tcatgagctt
     1021 tggctttttg ataaaaatca atggcgccaa agagattgcc atggtcggtc atggcgacgg
     1081 cgggcatgcc gaaattcttc acctgttgga acaggggtgt gagttggttg gccccgtcga
     1141 ggaggctata ctgggtgtga agatggagat gaacgaattg tgccggcact catatgatcc
     1201 ttccgtaaaa aaattctaat gagcacttcg agtgaagtca aatgaaatca ccatctgaaa
     1261 aatatcgcgg tgtccggttt ctgaattttt tgagaattgc aagaataaaa gacaaaatta
     1321 tgaggagaaa taaggataaa gtaatccaga aaaaatgggc acaagtatgt ggataaatcg
     1381 gcagtatccg attgaaaggc aagagagaga atgtgttaag cagattgctt atttggtggg
     1441 cattgtattg aacgatgaaa cccctaccac aggttgggtc atcaggatgt tcagaaaaat
     1501 atctgattta agagtcaaat tctgcgccat tcgttctcga ccatttgggg actgttgaat
     1561 attgtaaaaa ttttgctatt tcattcgctg ctgaggcacc gaggcccacc agaaaatagt
     1621 ggagtgttca aaaaggccgc tagcggcatt tttcaacact cccctttgtc gtgcagacat
     1681 actggcatgc gcgcgcgctt tgcggaaaag ttgtgagggg aaacccagga tgaatatagt
     1741 atagtcagca ggagagagcg gatctgaagt atgtgttttt catgtaaaag gagtgggagg
     1801 acatgatact gacaacgacg cacaatgttg aaggccatca gattaaagac tatttaggtg
     1861 tggtcgccgg agaggctatc ttgggcacta attttttcaa ggattttttt gccaatattc
     1921 gtgatattgt tgggggccga tcaggggcat acgaaaagga attgcgacgc gcgcgtgaaa
     1981 ttgcctttca ggaaattcag gaag
//
LOCUS       DAORAQ010000364         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8125_length_1996_cov_77.228748, whole genome shotgun sequence.
ACCESSION   DAORAQ010000364 DAORAQ010000000
VERSION     DAORAQ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8125_length_1996_cov_77.228748"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1091
                     /locus_tag="PKM72_15300"
     CDS             114..1091
                     /locus_tag="PKM72_15300"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNP62212.1"
                     /translation="MPKIIPDPTVEVVLPPNFNHEYFAEASQHPFRFKARRFQLVNAW
                     WLAEAALLAYAENEFAIPQYSKVGLNVEGNQPFSRGGSTQCYVAHNQDVVIVAFRGTQ
                     VPKPVAGKLPGEIWRQVVKDLWTDGKFRLVVSGQGGSVHEGFKMALDEVWEPLKSYLD
                     GLKEEKPTRTFWFTGHSLGAALATLAADRYGNAQGLYTFGSPLVGDEGFARDFYVGGY
                     RFVNNNDVVARIPLWGPSAMNLMKWGRYEHVGLLEYIDEAGMLFDNPSMLERLQHGVG
                     GQVQLLRQLMNQWANGDFGAIPIDCFNDHAPLYYALRIWNCYEQELQGV"
     gene            1698..>1996
                     /locus_tag="PKM72_15305"
     CDS             1698..>1996
                     /locus_tag="PKM72_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNP62213.1"
                     /translation="MGSKLYVGGLPYSATQAELTDLFSAHGTVESANVISDKFTGQSR
                     GFGFVEMSSGEEAQAAISALNSTEFGGRTLTVNEAKPQAPRTGGGGYGGGGGDFGG"
BASE COUNT          468 a          462 c          529 g          537 t
ORIGIN      
        1 cagtttcccc agcaatgcct catgccttcc ttggaaaagt aaatggcatc tgaatcattc
       61 tatggttctg tttttaatcc catgtaggtt cagtccataa ggaattccaa tatatgccaa
      121 agattattcc tgatccaact gtagaggtgg tgttacctcc aaatttcaat catgaatatt
      181 ttgccgaggc cagccagcat ccgttccggt ttaaggctcg aaggtttcaa ctggtgaatg
      241 cctggtggtt ggccgaggcc gccttgttgg catatgccga gaatgaattt gcgattcctc
      301 aatactcaaa ggtgggctta aacgtggaag gcaatcagcc ctttagccgt ggtggaagta
      361 cccaatgcta tgtggctcac aatcaggatg ttgtgatagt ggcctttcgc ggcacgcagg
      421 tgccgaagcc ggtggcaggg aaacttcctg gtgagatttg gcgtcaggta gtaaaagatc
      481 tgtggacgga cgggaaattc cgtttagtcg tatctggtca aggtgggtct gtgcatgaag
      541 gctttaaaat ggcgttggac gaagtttggg aacccctcaa gtcatattta gatggattga
      601 aagaggaaaa gccgactcga acattttggt ttaccggcca tagccttggc gcggctctgg
      661 caacattagc tgctgaccgc tatgggaatg cgcaagggct ctataccttt ggatctccgc
      721 tggtagggga tgagggtttt gccagagatt tctatgtggg tggatatcgg tttgtgaaca
      781 ataacgatgt ggttgctcgg ataccactct ggggtcccag tgcgatgaac ctgatgaagt
      841 ggggtcgcta tgaacatgtc ggtctcctcg aatacatcga tgaagctggc atgcttttcg
      901 ataacccctc catgttggaa aggctacaac atggtgtggg agggcaggtt caactccttc
      961 gccagttgat gaaccaatgg gccaatggag actttggggc catacccatt gattgtttca
     1021 atgatcacgc tcccctgtat tatgcccttc gtatttggaa ttgttatgag caggaactcc
     1081 aaggtgtcta ggagtagatt tccccaaggt ttccaatgtg aatgcggtat ctgtgacttc
     1141 attccatcat gggaagccac gcttatcccc ctctcttcgc aacgacaaat cagtatctaa
     1201 gccaggtagc tgaggccaaa tccgtccatc gggccgggaa cagcctgacc gttccggaaa
     1261 tcctgggaaa aatagcatgg aagccgatga gaaattccgg tttggatacg gtatttctga
     1321 cgtgtttccc cactgcactt ttgtgccagg aagggttggc tttctcgtag aatgctgaaa
     1381 atgctgaaga aaaggacctt cttgcctcaa ttgctcagag ccaggtccat atttcagtca
     1441 aaattttccg ttcccttcta ctggaattgg ctatcttctt cccttctctt gtgctagttt
     1501 gaatgaactt aacgcctagg gcttggcgat gagcaatcaa attaccaaag cccgtaaccg
     1561 gtgttgtttt gcccgagaga taactcgaaa gtgtctgtcc actgtcgcat gaatgcagcg
     1621 tccgcggcct actccttctc caggttttac cctttctcga gcctaagaca atatcatttt
     1681 tcaaaaggag gacccccatg ggttcaaaat tgtatgtagg tggtttgccg tattccgcga
     1741 ctcaagcaga attaaccgac ttgttttcgg cacacggcac cgtcgagtca gccaatgtca
     1801 tcagcgacaa gttcaccggc caatcgcgcg ggttcggatt tgtcgaaatg tcttcgggag
     1861 aggaagcgca agccgcgatt tcagccctga actctaccga gtttggtggc cgcactttga
     1921 cggtcaacga agctaaaccc caagctccac gcacgggtgg tggagggtat ggtggcggcg
     1981 gtggagattt tggcgg
//
LOCUS       DAORAQ010000365         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8192_length_1985_cov_86.200518, whole genome shotgun sequence.
ACCESSION   DAORAQ010000365 DAORAQ010000000
VERSION     DAORAQ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8192_length_1985_cov_86.200518"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PKM72_15310"
     CDS             <1..1542
                     /locus_tag="PKM72_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62214.1"
                     /translation="IMGKIYNVLTNGDDTVPKSEDNFFSWCTPGLAVEPDDFDFLTQG
                     LTGVVKKKALKDMVIEGEGGESNTPELTPAVLEGLRAQDASKQYMNAESFARLVDFVP
                     DLAATTNNQFAAMSIMNNEGSLSERFEYILRMSQIMETKLDDKTKEKIEKFRGLLSTT
                     RVKKNLVTDEEVEVSEPSALVNLYNQKLTEYEAAALAYNGARIDALTADNVKAVHYWG
                     MNANILRNRVKAAMGDWVSNGYKNDYEGINAFIDQVMRRDMALLKAQYQDDLEKARIT
                     GISSGSDFFYTSLVPSNPMQAAGWTNFSFQSTDYNNSSNSSYQMKRSKTSAGGGWLGI
                     FGGGGSASSASGQSESKVTFDSNHFYMNFSITQCQIVRPWFKTSFINSKLWRFDQNNP
                     ESSKQIVSDGGKPATGLCPAYPTAIIFIKDLVIGCRNSSGFSEAAQSWQRSSASGGGA
                     VHFGPFHFGGSHGRSSASGERSSKFHYDSEKQEMRVPGHQIIGFKCHVLPKSPDPLSE
                     ITDWI"
     gene            1639..>1985
                     /locus_tag="PKM72_15315"
     CDS             1639..>1985
                     /locus_tag="PKM72_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62215.1"
                     /translation="MAIDSIVQLALMSKAKKVFEKEGTFLSFPVTPLAYTKEDLDFFS
                     QETGSDLLQSKANLAAFSTLVNLIPRDEAWLPTDARFLWNELEYVLREGNLALSTRTQ
                     EEEVAYREALTFL"
BASE COUNT          560 a          421 c          512 g          492 t
ORIGIN      
        1 attatgggga aaatctacaa tgtgctgacc aatggggatg ataccgtccc caaatcggaa
       61 gacaattttt ttagttggtg cacacccgga ttggctgttg aacctgatga ttttgatttt
      121 ttaacacaag ggttaacggg tgtggtgaaa aagaaagctc tgaaggacat ggtgatcgaa
      181 ggtgagggag gggaatcgaa tacaccggag ctgacaccgg cagtcttaga aggattgcgt
      241 gcgcaagatg ccagcaaaca gtatatgaat gcagagagct tcgcgcggct tgtcgatttt
      301 gtccccgacc tggcggccac aacgaataat caatttgcgg ccatgagcat catgaacaat
      361 gagggatcgc tttcagagcg gttcgaatac attttacgca tgagtcaaat catggaaacc
      421 aaattggatg acaaaacaaa agaaaaaatt gaaaagttca ggggactgct ctcgacgaca
      481 agggtcaaaa aaaatcttgt gactgacgag gaggttgagg tttcagaacc cagtgcctta
      541 gtgaatctgt ataaccaaaa gctgacggaa tatgaggcgg cggcattggc ctataacggt
      601 gcccgcatcg atgccctgac agccgacaat gtcaaagcgg ttcattattg ggggatgaac
      661 gccaacattc tgcgcaacag ggtcaaggcg gctatgggtg attgggtcag taatgggtat
      721 aaaaatgatt atgaaggcat taacgctttt attgatcagg ttatgaggcg ggatatggcg
      781 ctgctcaaag cccaatatca ggatgatttg gaaaaagcca gaatcaccgg gatatcctcc
      841 ggcagcgatt ttttctatac ctcgctggtg cccagtaacc ccatgcaggc cgcaggatgg
      901 acaaacttta gttttcaatc cacggattat aataattctt ccaattccag ctaccaaatg
      961 aaacggtcta aaaccagtgc cggaggcgga tggttaggca ttttcggcgg tggcgggtct
     1021 gccagcagcg ccagtggaca atcggaatcc aaagtcacat ttgacagcaa ccatttttat
     1081 atgaattttt cgattaccca atgccaaatt gtgaggccgt ggttcaagac aagttttatt
     1141 aatagtaagt tatggcgttt tgatcaaaat aatcccgaat cctcaaagca aatagtcagc
     1201 gatggcggca aacctgctac aggactctgc ccggcttatc ccaccgcaat cattttcatc
     1261 aaggatcttg tcattggctg ccggaatagc agtggctttt cagaggcggc acaaagttgg
     1321 caacggtcgt cggcgagtgg tggtggggcc gtgcattttg gtccatttca tttcggtgga
     1381 tcgcatggcc gtagttccgc cagtggagag cgttcctcaa aattccacta tgattcagaa
     1441 aagcaagaaa tgcgagtgcc gggtcatcaa atcattggat ttaaatgcca tgtcttacca
     1501 aagagccctg atccgttaag cgaaatcacg gattggattt aactttccag gtagccccgc
     1561 cccaaaatta aaaaaggggt ggggctacca taggagaaaa caatattcca ccaccatatc
     1621 gacttgatga ggtaatacat ggctatcgac tccatcgtgc aattggcctt gatgtccaaa
     1681 gccaaaaagg tctttgaaaa ggaaggaacg tttttgagtt ttcctgtgac accgttggcc
     1741 tataccaaag aggatctgga tttcttttca caggaaaccg ggagtgatct tctccagagc
     1801 aaggccaacc tggcggcttt ttccacgctc gtgaatctga ttccccgtga tgaggcctgg
     1861 ttgcccactg atgcacgctt tttgtggaat gagttggaat atgttctgag agaaggaaat
     1921 cttgcattgt cgactcgtac acaggaagaa gaagtagcct atcgggaggc cttgaccttt
     1981 ttgaa
//
LOCUS       DAORAQ010000366         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8238_length_1976_cov_56.416970, whole genome shotgun sequence.
ACCESSION   DAORAQ010000366 DAORAQ010000000
VERSION     DAORAQ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8238_length_1976_cov_56.416970"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..705
                     /locus_tag="PKM72_15320"
     CDS             31..705
                     /locus_tag="PKM72_15320"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNP62216.1"
                     /translation="MMQSVYRRSVQVFFLVTGLLTLNLTGLACADSNMGAISDEAKRA
                     ESRAEKRALIVIDDYIIGPEDILEISVWRNPDLSREVMVRPDGKISLPLIGDVKAVGR
                     STSELRDDITQKLKSYKENPTVAIVVKAVNSYYFYVQGAVGKIGKLPLLSRTTLIQAI
                     TLAGGMAPDAVRSRITIFRLGMDNEAPQKLVVSYDDIILRGADNVELKPGDTIVVPSE
                     TMVLIP"
     gene            736..1347
                     /locus_tag="PKM72_15325"
     CDS             736..1347
                     /locus_tag="PKM72_15325"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNP62217.1"
                     /translation="MRNTFPSIGSKVCILLMGFLTCMGCLKPDVVVSPGHPEEGFVLG
                     PEDVIEVIVWKNPDLSRQVVIRPDGKISLALIGDVVASGLTADQVAKKIAEKFKAVKE
                     NPSVSVNVIEVNSYYVFIVGEVVKPGKLPLKSYTTILQAMSLAGGFTQFASRNDIIVV
                     RTEKDEKGHLIETRIPLRYSDLISEDGGVYNITLRSGDTVIVP"
     gene            1424..>1976
                     /locus_tag="PKM72_15330"
     CDS             1424..>1976
                     /locus_tag="PKM72_15330"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNP62218.1"
                     /translation="MSGPTAAHNNTDPALIVQKYLKDFWDLAVRRKWLILSFVILGIA
                     IGGTLAWLKVDMYRSETVILIEQQKIPEKYVSSVVGGSTADRVSTMTQQVLSRTSLLK
                     IIEEFHLYSDDIKARGYEGVIEALRTNILIETKGSGGQIEAFNISFAHRDPKVAMKVT
                     ERLASQYIDENIKIREQFIEGAME"
BASE COUNT          528 a          373 c          541 g          534 t
ORIGIN      
        1 ggagaacttt cacggggaat gaggtgagtt atgatgcaga gtgtgtatcg taggagtgtc
       61 caggtcttct ttctggtaac tggtttgttg actctaaacc ttaccgggtt ggcttgtgct
      121 gattcaaaca tgggtgcaat atccgacgag gcgaaacgag ccgaaagtcg tgccgaaaaa
      181 cgggcattaa ttgtgattga tgattacatc attggtccgg aagacatttt ggaaatcagt
      241 gtttggcgca atcccgattt gtcgagagaa gtgatggttc gacccgatgg gaaaatttcg
      301 ttgccactga ttggggatgt gaaagcggtg gggcgctcga cttctgaact tcgcgatgac
      361 attacgcaaa aactcaagtc ctataaggaa aatccgacag tggccattgt ggtcaaggca
      421 gtgaatagct attactttta tgtgcaaggg gccgtcggaa aaattggaaa attgcctttg
      481 ttaagccgaa cgactcttat ccaggccatt acattggcgg gtggaatggc gcctgatgcg
      541 gtgcggagtc ggattaccat ttttcgattg ggaatggata acgaggcacc tcagaaatta
      601 gtggtcagct atgatgatat tattttgagg ggagctgaca atgtggagtt aaagcccgga
      661 gatacgattg tcgtgccttc tgagaccatg gttctgattc cgtaaatact cattttttag
      721 tttgattgag ggcatatgag gaataccttt ccatccatag gcagcaaggt gtgtattctc
      781 ctgatgggtt ttctgacctg tatggggtgt ttaaagcctg acgtggttgt ttcaccaggt
      841 caccctgaag agggctttgt attgggtccg gaagatgtca ttgaagtgat cgtctggaaa
      901 aacccggatt tgtcgaggca ggtggtgatc cggccggacg gaaaaatttc tttagcctta
      961 atcggtgatg tggtagccag cgggttgacg gctgatcagg tcgccaaaaa aattgcagaa
     1021 aaatttaagg cagtaaaaga aaatccttcg gtgtctgtga atgtgatcga agtcaatagt
     1081 tactatgtct ttattgttgg agaagtggta aagccaggga aattgccatt gaaatcctat
     1141 acgacgatcc tccaggccat gtctttagcc ggtggattta cccaattcgc ttcccgaaat
     1201 gacatcattg tcgttcgcac ggaaaaagat gagaaggggc atctcataga aacacgtatc
     1261 ccgcttcgat attccgatct tatttcggag gatgggggag tgtataacat cactctccgg
     1321 tccggggata cagtcatcgt cccgtaaatc aggaagccgc ttaaaaaccg gattggccag
     1381 gtagatcctg ggatctcgca atagaaagaa tatcatgggc tccatgtctg gtcctactgc
     1441 agcgcataac aacaccgatc ctgcacttat tgtccaaaaa tatctcaaag atttttggga
     1501 tttggcggtt cgacggaagt ggttgattct gagttttgtc atactgggaa tagccattgg
     1561 tggaactttg gcttggctga aagtggatat gtatcgctcg gagacagtca ttctcatcga
     1621 acaacaaaaa attcctgaaa aatatgtctc gtccgtggtc ggaggcagta ccgcggaccg
     1681 cgtgtccact atgacccagc aggtgttaag ccgtaccagt ttgctgaaaa tcatagaaga
     1741 atttcactta tactctgacg atataaaagc ccgggggtat gagggtgtga ttgaggcatt
     1801 acgaacaaac attctgattg aaacgaaggg gagtggtggg caaatcgagg cttttaacat
     1861 atcgtttgcg caccgtgatc cgaaggtggc catgaaagtg acggaaaggt tggcttctca
     1921 atatatcgat gaaaatatca agattcgcga acaatttatt gaaggggcga tggagt
//
LOCUS       DAORAQ010000367         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8295_length_1968_cov_66.083116, whole genome shotgun sequence.
ACCESSION   DAORAQ010000367 DAORAQ010000000
VERSION     DAORAQ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8295_length_1968_cov_66.083116"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1200)
                     /locus_tag="PKM72_15335"
     CDS             complement(271..1200)
                     /locus_tag="PKM72_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNP62219.1"
                     /translation="MHANTNKDSGILLLDSFTPSMSRLRKIRRILCWILVLNLAVAAA
                     KWGYGVVTDSLGMQADGFHSTFDGFSNVIGLIGLWLTAPPPDANHPYGHKKFEALTAG
                     AIGGFLVMTCVYLLWKAYQSWTLDLHPQVTGFSFLIMIMTMGVNVFVTRWERKKATEL
                     KSDILKADSYHTASDVLTSCSVLVGLLAVKVGYAFVDPLVAVLIAAVIAWTAFIVLKD
                     VLSSLTDANRLDPEEVRKVVMSIAGVRDCHDIRTRGLSHHVFMDLSVHVDANLTVKKS
                     HSLATQIEEQLIEMFEPLEDVVVHIEPEGHERR"
     gene            complement(1205..1900)
                     /locus_tag="PKM72_15340"
     CDS             complement(1205..1900)
                     /locus_tag="PKM72_15340"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HNP62220.1"
                     /translation="MRMRHSERHRTDRIGWLRAAVLGANDGIVSTASLLLGVSAAHAT
                     HSDVLIAGVAGLVAGAMSMAAGEYVSVQSQADTEKADLALEHTELQSDPSGEHKELAA
                     LYVERGLDPAMAKQVAAQLMAHDAIGAHARDELGISENLRARPVQAAMASAGSFAFGA
                     ALPLLTATITPEASLIPVVSGTSLVFLAGLGALAAQAGGASVMTGAMRVTLWGALAMG
                     LTAGVGTFFGAAG"
BASE COUNT          453 a          545 c          526 g          444 t
ORIGIN      
        1 atccgttttt ttccaccaag gccgtggggg aggggaccgg cttagggctc accgtggttc
       61 atggcattct ccaggaacat caaggtgcca tccgtgtaac gagtgtgccc ggccagggga
      121 cgacctttat cgtttccctg cccattcatt ctcaagaggt aaggcgtgag gggtaggtcg
      181 agcatgagtc taagtttaat ctgctgaatg cttcattttc cctttttctc cttactgctt
      241 actcctcaca atttcccatc ccgattccaa tcaccttcgt tcgtgtcctt ccggttcgat
      301 gtggacgacc acatcctcca ggggttcaaa catctcaatg agttgctcct caatttgggt
      361 tgcgagcgag tgcgattttt tgacggtgag attggcatct acgtgaaccg agagatccat
      421 gaacacatgg tgggacagtc cgcgtgtcct aatatcatga cagtcccgga cgccggcgat
      481 cgacatcacc acttttcgaa cttcttcagg gtccaagcga ttggcatcgg ttaaggaaga
      541 caaaacgtcc ttgagtacga taaatgcggt ccaggcgatc acggcagcaa tcaggaccgc
      601 taccaacgga tcaacgaagg catatccgac tttcacagcc aggagcccta cgaggactga
      661 acatgatgtg aggacatcac tggccgtgtg ataggagtcg gctttgagaa tgtcgctttt
      721 cagttcggtg gcctttttcc gttcccatcg ggtcacaaaa acattgacac ccatggtcat
      781 gatcatgatc aagaaactga agccggtgac ctgagggtgc aggtccagtg tccaagactg
      841 ataggctttc caaagaagat acacacatgt catgaccagg aatcctccaa tggctccggc
      901 ggtcaaggct tcaaattttt tatgaccata gggatggttg gcatcgggag gaggggcggt
      961 cagccagagc ccgatcaatc caatgacatt tgagaagcca tcgaaagttg aatgaaaacc
     1021 atccgcctgc atacccaatg agtccgtcac aacgccatag ccccacttgg ctgcggcgac
     1081 agcaaggttc aaaaccagaa tccaacagag aatccggcga atttttcgaa gacggctcat
     1141 gcttggtgta aaggaatcaa gaagaagtat gcccgagtct ttgttcgtgt tcgcatgcac
     1201 tcatttatcc cgctgcaccg aaaaatgtgc caacccctgc ggtcagtccc atggccagtg
     1261 caccccataa tgtgacgcgc atggcgcccg tcattaccga cgcaccgccc gcctgtgcgg
     1321 ctaacgctcc taatcccgcg agaaacacca gtgatgttcc ggaaaccacg ggaatcaggc
     1381 tggcctccgg ggtgatggtt gcggtcagaa gaggcagggc ggctccgaaa gcgaagctcc
     1441 cggccgacgc catggcagcc tgaaccggac gcgcccggag gttttcggaa atgccgagtt
     1501 cgtcacgtgc atgcgctcca atagcatcat gggccatgag ttgcgcggcg acctgtttcg
     1561 ccattgctgg atcgaggcct cgctcaacat aaagagcggc gagttccttg tgctcaccgg
     1621 aaggatccga ttggagttcc gtatgctcaa gggcaaggtc ggctttttcg gtgtcggcct
     1681 gtgattggac agagacatac tcaccggcag ccatcgacat ggctcccgcg accaggccgg
     1741 ctactcccgc gatcaagaca tcgctgtgag tggcatgtgc cgctgacaca ccgagcagca
     1801 ggctggctgt cgatacgatc ccgtcgttgg cgcccaagac tgctgcccgg agccatccga
     1861 tgcgatcagt cctatggcgt tcactatgtc gcattcgcat gtgtctattt tccactaagt
     1921 gcaggggctc actaccaaaa ccggacttct caagatttgt ttactcag
//
LOCUS       DAORAQ010000368         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8399_length_1956_cov_67.347186, whole genome shotgun sequence.
ACCESSION   DAORAQ010000368 DAORAQ010000000
VERSION     DAORAQ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8399_length_1956_cov_67.347186"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /gene="sucC"
                     /locus_tag="PKM72_15345"
     CDS             <1..185
                     /gene="sucC"
                     /locus_tag="PKM72_15345"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNP62221.1"
                     /translation="AHGVIDAAKEVGIKLPVVVRLQGTNAEEGRKLLAESGLKVEGVA
                     DLWEAAQRIVALRKKK"
     gene            236..1108
                     /gene="sucD"
                     /locus_tag="PKM72_15350"
     CDS             236..1108
                     /gene="sucD"
                     /locus_tag="PKM72_15350"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNP62222.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTQVVAGVTPGKA
                     GQEVEGIPVFNTVREAVKKTEATTSLIFVPPPFAADAILEAADAGIWLIICITEGIPV
                     NDMVRVQRALYGKTTRLIGPNCPGIITAEECKIGIMPGFIHKKGRVGVISRSGTLTYE
                     AVNQLTNLGLGETTCVGIGGDPLIGTGYIDLLHMFQEDDETEAVVMIGEIGGDAEERA
                     AAYIQKEMTKPVVSFIAGITAPPGRRMGHAGAIITGGKGTAADKMAALESAGVIVVKN
                     PAEIGEAVRSVLHA"
     gene            1487..>1956
                     /locus_tag="PKM72_15355"
     CDS             1487..>1956
                     /locus_tag="PKM72_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNP62223.1"
                     /translation="MSKLHNNSATFFTWRIVGGLLGSLLVFPLLLFAQEPSIPSSDET
                     TDVFILRQLIEIQNELKDVRKELAELRQSVDESKKNFNLPLAPASLPSVVGNVELKDH
                     DPTLGNQRAKVAIVEFTDYQCPYCAQYHSETFENLKKEYIDTGKVQYVLRDFPL"
BASE COUNT          530 a          432 c          489 g          505 t
ORIGIN      
        1 ttgcccatgg agtgattgat gcggcgaaag aagtcggcat caagctacct gttgtcgttc
       61 gacttcaagg aaccaatgcg gaagaaggcc gaaaacttct ggctgaatca ggcttgaaag
      121 tggaaggggt cgctgacctc tgggaagcgg cacaacgcat tgtcgctctc agaaaaaaga
      181 aatagtcaac ctgattgtga aattattctc cttgattggt gaaaggtgac gtggcatgag
      241 cattttggtg aataaaaata cccgggttgt cgttcaagga atcaccggca aagaagggtc
      301 ctttcatgcg acccaatgca aagcgtatgg aacgcaggtt gtggccggtg tgacgccagg
      361 caaggccggg caagaagtcg agggcatccc ggtgtttaat accgttcggg aagccgtcaa
      421 gaagacggaa gccaccacgt cgttgatctt tgtgccacct ccgtttgccg ccgatgcgat
      481 tttagaggcc gccgatgcgg ggatatggct catcatctgc attacagagg ggattccggt
      541 caatgatatg gttagggtcc agcgggcact atatgggaaa accacgcgtc tgattggccc
      601 caattgtccg ggaattatca cggccgagga atgcaaaatc gggatcatgc ctggatttat
      661 tcataaaaaa gggcgggtcg gcgtaatttc acgaagtggc accttaacct acgaagccgt
      721 caatcagctc accaatttgg gtcttggcga aacgacctgc gtcggcatcg gtggcgaccc
      781 cctcattgga accggatata tcgatttgtt gcatatgttc caggaggatg acgaaaccga
      841 agcggtggtg atgatcgggg aaattggtgg agatgccgag gaacgggctg ccgcctatat
      901 tcaaaaagag atgaccaagc cggtcgtgag tttcattgcc ggaataacgg cacctcccgg
      961 aagacgaatg ggccatgccg gtgccattat taccggagga aagggcaccg ccgccgataa
     1021 aatggccgcc ttagaatctg ctggggtgat tgtcgtcaaa aatccagcag aaatagggga
     1081 agccgtgaga agcgttctac atgcctagcc ttattcgttc ttaacgcctc ttcattttct
     1141 tcataagttt aatcccgttc catgaactga gtggacacgt ttgtcctgat gagaacatgg
     1201 agtttgcgga attttagcaa cttcttccac aaaaccccgc aaatccaatt tttcgcttac
     1261 tttcccaaaa atttgtgtcc tgcttcagct gggaatcact agaactatta ggtgaaggcc
     1321 atgcaaaggc agggaaaatt ctgaacgtat tatgtggtgg ggcaataatt atcctcaata
     1381 tctgtccccg ttatatttga agtcttatag agttccatta ttttggcaag attctttatt
     1441 ttatttaaaa agaatagtaa ggtctttcac tggaaagtga ggacctatgt caaaactaca
     1501 taataattct gcgacatttt tcacgtggcg gatagtcgga gggctactcg gatcccttct
     1561 ggtctttccc ctcctgctct ttgctcagga accttcaatt ccttcctccg atgaaacaac
     1621 agatgttttt attcttcggc aactcatcga aattcaaaac gaactgaaag atgttcgaaa
     1681 agaacttgca gaattacgtc aatcggtgga cgaatcaaag aaaaatttca atcttcccct
     1741 tgctcctgct tcacttcctt ctgttgttgg caatgttgaa ctcaaggatc atgatcctac
     1801 ccttggtaat cagcgagcaa aagtggcgat agtggagttt acggattatc aatgtcccta
     1861 ttgtgctcaa taccattcag aaacatttga aaacctgaaa aaagaatata tcgacacggg
     1921 aaaagtgcaa tatgtccttc gagattttcc gttgga
//
LOCUS       DAORAQ010000369         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8460_length_1947_cov_60.995243, whole genome shotgun sequence.
ACCESSION   DAORAQ010000369 DAORAQ010000000
VERSION     DAORAQ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8460_length_1947_cov_60.995243"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PKM72_15360"
     CDS             complement(<1..163)
                     /locus_tag="PKM72_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNP62224.1"
                     /translation="MPIYEYRCQDCRKRNSFLILTSNAPVPSCKHCLSANLERIMSRF
                     AAPKSEEARM"
     gene            complement(461..1201)
                     /locus_tag="PKM72_15365"
     CDS             complement(461..1201)
                     /locus_tag="PKM72_15365"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNP62225.1"
                     /translation="MTPLFSTRLLRSLLHVIFPTACAGCQNPLWEDPVPFFCRRCWDG
                     LKPIPDPVCPRCGRPFASSIALLHSPAHQCGACRARPLALTQVWSLFPYQSPLKEAIT
                     LFKYRGKLSLTHPLAQAMIEALPALPPLDAIISVPLHPQRLREREYNQSLLLANELRH
                     ATGIPLLLGCLLRIRPTVPQTSLSRKERLTNLRRAFSVAKPANIQGKRILLVDDVFTT
                     GTTLHECAKTLRRAGSGPVYGLTLARMV"
     gene            1356..>1947
                     /locus_tag="PKM72_15370"
     CDS             1356..>1947
                     /locus_tag="PKM72_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNP62226.1"
                     /translation="MIQFQHVYKEYRRGSTPVVALRDVCVDIQQGEFCALMGPSGSGK
                     STLLHLVAGLDWVTSGDILLDGRSCLAFGDQEWTRWRREQVGVVFQAFHLIPGLTIEE
                     NVALPLRLRGEVSGSVRTRTADILERVGMSHRLGHRSHELSGGEQQRVALARAFVHRP
                     RLIVADEPTGNLDGETGASIVSLLRQCAKEFGQTVLL"
BASE COUNT          467 a          416 c          620 g          444 t
ORIGIN      
        1 ccattctggc ttcttccgat ttgggtgcag caaagcggga catgatgcgt tccagattgg
       61 cactcaggca atgcttgcag gatgggactg gagcgtttga ggtgaggatt aaaaaggaat
      121 tacgtttccg gcaatcctga caacggtatt cataaatggg catcgagtcg ttctccttct
      181 gatcgcaatg tacgggctgt atatatcgca aacccggcca tttgggaaga gtggggtttt
      241 ggtttctgag aattgaaaga tctcatatga tttaatagag cgaatccctc tccctcattc
      301 tgggaagtct cacgaaaaga agatggtcaa aagggctgtt gagaaaggtg agggaaaggg
      361 agaagaaaag gatctgcaga ttcatttaga taaaacaacc agcagaagaa ccttactgga
      421 aacctcttcg ccttgatgca ggtattggga attaattccc ctacaccatt ctagcgagtg
      481 tcagcccata caccgggcct gatccggcac gccgaagcgt cttcgcgcat tcatgcaagg
      541 ttgtgccagt ggtaaagaca tcgtccacca ataaaattcg tttgccttga atgttggcag
      601 gcttggccac ggaaaaggct cgacgcagat tcgtgagtcg ctcttttctt gataatgaag
      661 tttgggggac cgtgggtcgg atcctgagca ggcatcccag taacagagga atcccagtgg
      721 cgtgacggag ttcgttggct agcagcaggg attgattgta ctcacgttcc cgtaatcgtt
      781 gcggatgaag ggggacggag atgatggcat ctaagggtgg taaagctggc agcgcctcaa
      841 tcatggcttg ggcgagtggg tgagtcaacg acagttttcc acggtatttg aatagggtaa
      901 tggcttcttt gagtggggat tggtagggaa agagactcca gacttgcgtg agggccaatg
      961 gtcgtgcgcg gcaggctccg cactggtggg cggggctatg aaggagggca atggaagaag
     1021 caaaagggcg tccacatcgc gggcaaacgg ggtctggaat aggtttgagc ccatcccaac
     1081 accggcgaca aaaaaatgga acgggatctt cccacaaggg attctgacag ccggcacagg
     1141 cggtgggaaa aatgacatga aggagcgatc ggagaaggcg agtcgagaaa agcggagtca
     1201 tggaggcgaa ctttcagagc tgaggccgac agggatgtaa acctaatatt cgtggggcgc
     1261 cttggtccat ttaccttcgg tatgatatag gagggatagc aaataaacca tgattcaagt
     1321 gcgtttctga ctgccgcatt tccggatggg tgcccgtgat tcagtttcaa catgtctata
     1381 aggaataccg gcgcggatcc actccggtcg tagcgttgcg ggatgtctgt gtggacattc
     1441 agcaaggaga attttgtgcg ctcatgggac ccagcgggag cggaaaaagc acgctattgc
     1501 atttggtggc ggggctggac tgggtgacat ccggcgacat cctgcttgac gggcgatcct
     1561 gcctggcgtt tggtgatcag gaatggaccc gatggcgtcg ggaacaggtt ggggtcgtgt
     1621 ttcaggcatt tcatctgatc ccgggattga cgattgaaga aaatgtagcg cttcccttgc
     1681 gtttacgagg tgaggtatcc gggtcggtgc gaacccggac agccgacata ttggagaggg
     1741 tggggatgag tcatcggctg ggtcaccggt cgcatgaatt atccggagga gagcagcagc
     1801 gggtggcgct tgcccgtgcc tttgtgcatc gccctcgact gatcgtggcc gatgaaccca
     1861 ccggcaattt agatggtgaa accggggcga gcattgtgtc gttgctgcgt caatgtgcga
     1921 aggaattcgg ccagacggtg ctcttgg
//
LOCUS       DAORAQ010000370         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8484_length_1943_cov_60.447034, whole genome shotgun sequence.
ACCESSION   DAORAQ010000370 DAORAQ010000000
VERSION     DAORAQ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8484_length_1943_cov_60.447034"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKM72_15375"
     CDS             complement(<1..386)
                     /locus_tag="PKM72_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNP62227.1"
                     /translation="MKPLEHAMIDKFKTHFGGEALLPGDAGYDEVRQIWNAMIDRRPA
                     LIARCVSTEDVVQAVGFARKHNLLVSIRGGGHNIAGNAVCDDGLMIDLSLMKNVQVDP
                     KARRATVEPGCTLADFDAAAQAHGLAT"
     gene            complement(557..688)
                     /locus_tag="PKM72_15380"
     CDS             complement(557..688)
                     /locus_tag="PKM72_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62228.1"
                     /translation="MMATPSKFSDSFLPSGTSTCFRILSVFSRYGLITNTGLRVAIR"
     gene            complement(1038..1409)
                     /locus_tag="PKM72_15385"
     CDS             complement(1038..1409)
                     /locus_tag="PKM72_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNP62229.1"
                     /translation="MSQQSLYERLGGYEGIVGFTNNLLPRLQSDPQLGRFWQNRGDDG
                     IARERQLLIDYLCANAGGPMYYTGRDMKLSHKGMKISERDWAIFLEHAGATMKALDVP
                     RQECDEVASFVSSLKPDIVEV"
BASE COUNT          436 a          575 c          502 g          430 t
ORIGIN      
        1 gtggcgaggc catgggcctg cgcggccgcg tcgaaatcgg caagggtgca gccgggctcg
       61 acggtcgccc ggcgagcctt cggatccact tgaacattct tcatcagcga gaggtcaatc
      121 atgaggccgt cgtcgcagac ggcattcccc gcgatgttgt gcccgccccc tcggatcgag
      181 acaagcaggt tgtgcttgcg cgcgaacccg accgcctgga cgacatcttc agttgacaca
      241 cagcgggcaa tcaacgcggg acggcggtcg atcatggcgt tccagatctg ccgcacctca
      301 tcatagccgg cgtcgccagg aagaagtgcc tcccccccga agtgtgtctt gaatttgtcg
      361 atcatggcgt gttctaacgg tttcatacct gcctcctttg cataggtctc acgtttttgc
      421 ctagtttttt atggaaacac cttcagaaaa gaccgtgagg cctgttgggc caattcctgc
      481 cggcctcgac gcccgcccaa acagtcgaac ggcaggcccg agagccattc ttccggcacc
      541 cgacagggcc acaaacctat cgaatcgcca cacgaagtcc ggtattggtg atcagaccat
      601 agcgggagaa tacggaaaga attcgaaagc aggtggatgt gccggaaggc agaaaagaat
      661 cggaaaattt cgaaggagtc gccatcatct gtttccacgg aggcggtatt ctataccaaa
      721 aataaccgcc tacgcatctt ccccccaaac agatcgctga acagcttgtc accgccattg
      781 agtgaagcag gaggagacgg gctacctcgc gaggctcgga caagcccgcc aagggaaaga
      841 gcgtccgatc atgggacggg cggaaggcgt cggacccaga gaacaacctc gtacctcaac
      901 acttccttat acgcgctgag ttggtgtcag cgtgatgttc tgctggggtt cgccccggca
      961 gctgagctaa tgcttggcgc gtcccaaagt gcgccacctt atagtggtgg actggataga
     1021 tcttgtcatc cctcgactta gacttcgacg atatccggct tcaagctgga gacaaacgac
     1081 gccacctcgt cacattcctg ccgcggaacg tccaacgcct tcatcgtcgc cccggcatgt
     1141 tcaaggaaaa tcgcccaatc acgttcgctg attttcatgc ctttatggga gagcttcata
     1201 tcgcggccgg tgtagtacat cggacctccg gcattggcac acaaataatc aataaggagt
     1261 tggcgctccc tggctatccc gtcatcacct cgattctgcc agaatcgccc caattggggg
     1321 tcggattgca accggggaag caaattattt gtaaacccga caatgccctc gtacccgcca
     1381 agacgttcat acagtgactg ttgactcata ctgttctcct catcgttgca aggtgaaaaa
     1441 agtaggctag actcaggaca tctctatccc gttgccaggc agattgatcc tgccctctcc
     1501 attagtgtgg attttctgtg gacaacacat tgggcaccat gcgccccatc gtccggccat
     1561 cgttgtatca ggtgatgttt ggattccaga tccgaagcat gagagttctg gttggccgaa
     1621 gatcgtcagt gttcggctaa cttcatttcc ctagaacaaa atttcaccat tggttttcac
     1681 agcccaatct cctttctcat gagtccggag actggcgcga aagatcagcc ccctcgtcag
     1741 cgatagggtt ggtcgtacgt ctcccgtccg gcctagaatt ctcgcaacaa tcttccccca
     1801 cccggtaacg gtccttcgaa tccgttttcc cgtaacgcgt accagaaggt caccgcgtta
     1861 atccccagga tgggaatccc tagcaccggt tccaatttct ccgtcacgtt gatcaagctc
     1921 atgttcgtgc cacactggac gac
//
LOCUS       DAORAQ010000371         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8507_length_1940_cov_68.189390, whole genome shotgun sequence.
ACCESSION   DAORAQ010000371 DAORAQ010000000
VERSION     DAORAQ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8507_length_1940_cov_68.189390"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1485)
                     /locus_tag="PKM72_15390"
     CDS             complement(310..1485)
                     /locus_tag="PKM72_15390"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNP62230.1"
                     /translation="MAIPKFLMKGLFLLGIIWMVFGHEFSMAEGFTPREECSPAQSEL
                     STPVPCTVRFLSYNVHGISPILNSMYVGVDVRNDDTRSGLMAWLLMDYDFILLQEDFE
                     FHRQIVDEFTYELIEQGNGPRIALAPLWLVTLPIKIVNVGFQLFNSPGIPFRVPYGSG
                     LTIASPYYKAVEGGVAQVPLPDCEGWFSRKNDCWSTKGLLRVPVRLPNGAEFDVYNTH
                     LDAGRDEEDRSVRKDQLDFIADCIKKLSKDRAFILGGDFNSQYDHEGTEASGEDYRIL
                     KRDLLDKLALNDSNARPEPNGYWKNRVDYIFFRGGQGARIELIDSGSEDQRFRHPVNR
                     GRQAPNEKYEDLLSDHPAIRAEFKILPSPISAHTSRGGERDSCAKKQPVENDMERIW"
     gene            complement(1631..>1940)
                     /locus_tag="PKM72_15395"
     CDS             complement(1631..>1940)
                     /locus_tag="PKM72_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62231.1"
                     /translation="KPAVQISTTLVIPLDKNVLRRILASHYHPDQTDGPSCLTCLGHT
                     KDSLWGMYFFRCESLPPKWKRPTALRRLLSFLVAIILSGIISDSKSSMISNSPGTALE
                     "
BASE COUNT          527 a          465 c          425 g          523 t
ORIGIN      
        1 gttgagttta taagatacct tccagagaaa cagcgagttc gaggtttttg accatactgg
       61 aagtcccatc atccgagaca aaacctgttt tcataattat tttaacggta gtctccaaac
      121 cccgccgact tgcgcaaatc gttgcttgaa aattatcaac tggcagagcc aaatatcact
      181 ttgggtttac ttaatatagg gcatctctaa aaactcctga caggcccgga aagggaggtc
      241 ctaaagctat gggttttttg aggtgcccta tagggttaat ttccttataa cgacaacatt
      301 tggtctcact caccaaatcc tttccatatc gttctcgaca ggttgtttct ttgcacacga
      361 atctctttcc ccaccccgtg aagtatgagc ggagattggc gagggtaaga ttttgaattc
      421 cgcgcgaatg gcagggtggt cgctaagaag atcttcgtac ttttcattag gagcttgtct
      481 gcctcggtta acaggatgcc tgaaacgctg atcttccgat ccagagtcga ttaactcaat
      541 ccttgcccct tgacctcctc gaaagaaaat gtagtccact cgatttttcc aatatccatt
      601 gggttctgga cgtgcattgc tgtcattgag agccaactta tcaaggagat cgcgttttaa
      661 aattcggtag tcttctcctg aggcctcggt tccctcatgg tcatattgtg agttgaaatc
      721 acccccgaga atgaaggccc gatctttcga taattttttg atacagtctg caatgaaatc
      781 aagctgatct tttcggacgg atcggtcttc ctcatctctt ccggcatcaa ggtgagtatt
      841 gtacacatca aattctgcgc cattcgggag acgaaccggc actcgaagaa gccccttggt
      901 cgaccagcaa tcattttttc tactaaacca tccttcacaa tctggaagcg gtacctgtgc
      961 gactccccct tccacagctt tatagtaggg tgaagctatc gtcagcccag agccataggg
     1021 aacacggaaa ggaataccgg gactattaaa taactgaaaa ccaacattga cgattttaat
     1081 gggaagggtt acgagccata gtggggccaa agcgatacgt ggcccgttcc cctgttctat
     1141 gagctcataa gtaaattcat cgacaatttg ccggtgaaat tcaaaatcct cttgtaataa
     1201 tatgaaatca taatccatga gcaaccaggc catcaggcca cttcgggtat catcatttct
     1261 cacatctacc ccgacataca tggaattcag gatcggagaa attccgtgaa cattgtacga
     1321 taggaaccga acggtacagg gcacaggtgt cgatagttcc gattgggcgg gagaacattc
     1381 ttctcttggc gtaaaacctt ctgccattga aaactcatgc ccgaatacca tccatataat
     1441 gccaagtaaa aagagccctt tcatcaagaa ttttggtatg gccatctttg acaactcctt
     1501 tttatgccag aactcttatc aataacgact aggtccttag acgaactaga gactacagcc
     1561 ccaacgatga aaccactcct actgtacctt cctcagctca aaaatcaagt gcccctcaca
     1621 tgcattcaca ttattctaag gctgtccctg gtgaattgct gatcatgctg ctcttggaat
     1681 ccgaaataat ccctgacaat atgattgcca ccaaaaatga aagaagtcta cggagtgcgg
     1741 tgggcctttt ccatttgggt ggcagcgatt cacaccggaa gaaatacatg ccccacaggc
     1801 tatcctttgt gtggccgagg caagtcagac acgaggggcc atcggtttga tcggggtgat
     1861 agtgagaggc gaggattcgt cgcaggacat ttttatcgag aggaatgacc aaggttgtgg
     1921 atatctgtac ggcaggtttc
//
LOCUS       DAORAQ010000372         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8609_length_1926_cov_67.820951, whole genome shotgun sequence.
ACCESSION   DAORAQ010000372 DAORAQ010000000
VERSION     DAORAQ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8609_length_1926_cov_67.820951"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="PKM72_15400"
     CDS             complement(<1..1853)
                     /locus_tag="PKM72_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNP62232.1"
                     /translation="MLHIEQISKSHPTKPLFIKASAHLKPNTRVGLVGPNGSGKTSLM
                     RMILTEEEFESGKIRQRPFLRLGYLPQELESLPGNTILDATHRNQFPDHEAKRILAGL
                     GFQESDWERKIDTLSGGYRMRVALAHLLLSAPDVLMLDEPTNHLDKPTQRWLESFLLT
                     SNFTLLIISHDTGFLDNMVTHIWEVRNHTLQEYRGNYTRFQKLRAERDAQEEAAANRQ
                     SKEISRVQNFVDRFRYQANKASQVQSRIKQLEKVKRIELQRDTKRLRFKFPDPPASGK
                     DVFELRGIGKRYDEKIIYDGLDFSVERGQRVALVGENGAGKSTLLKILAGVLEFDKGS
                     RTLGHGVTLHYFAQHQAEILKSEHSILDSLEEAAPMTERNFLRGLAGAFLFSGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPTSVDMLTDALLQFPGTMVFIS
                     HDPTFLMRVATRVVEIDDGQARDYIGDYEYYCWKRAKELEDQVPPEAKDDKKKSKKKE
                     KPQKEKPTTEHSTNGTARTTVAPPDGPSRRDLSKSIARLEKQVTTVEKDIAQLEEQIK
                     ARDVELADPATYQDYSRWNSLHHEREQWGHDLDRLTHKWGDLSAQLEKQQS"
BASE COUNT          392 a          451 c          542 g          541 t
ORIGIN      
        1 tgggattgtt gtttttcgag ttgggcgctg aggtcgcccc atttgtgggt gagacgatcg
       61 aggtcatggc cccattgttc gcgttcgtgg tggagggaat tccatcggga gtagtcttgg
      121 taggtggccg ggtcagcgag ttccacatcc cgggctttga tttgttcttc gagctgggca
      181 atgtcttttt ccacggtagt gacttgtttt tccaaacggg cgatgctttt tgagaggtct
      241 cgtcgagacg gaccgtcagg aggtgcgacg gttgttctgg ctgtcccatt ggtggaatgc
      301 tcggtcgtgg gtttttcctt ttgaggtttt tcttttttct tggatttttt cttgtcgtct
      361 ttggcttccg gcggaacctg gtcttcaagt tctttggccc gtttccagca gtaatattca
      421 tagtcgccga tgtaatcgcg ggcttgtcca tcgtctattt ctacgacgcg tgtagccaca
      481 cgcatgagga atgtggggtc atgcgagata aacaccatgg tccccggaaa ttgcagcagg
      541 gcgtccgtca gcatatccac ggatgtggga tccaaatggt tggtcggctc atctaagagc
      601 aaggtattgg ccggttcgac gagcatacgg gctaaggcga ccctgttgcg ttccccgccg
      661 ctcaaggctt tgatgggttt tttctgatca tctcctgaga ataaaaatgc gccggctaaa
      721 ccccgtaaga agtttcgctc agtcatgggc gcggcttctt ccagtgaatc caatatggaa
      781 tgttctgatt tcagaatttc ggcctggtgc tgggcaaagt agtggagggt gactccgtgc
      841 cccaaggttc tggacccttt gtcgaattct aaaactccag ccaggatttt gaggagcgta
      901 gattttccag ccccgttttc tccgactaag gccacgcgtt gtcctcgctc aaccgaaaaa
      961 tccaagccat cataaatgat cttctcgtca tagcgtttcc cgataccccg tagctcgaac
     1021 acgtcttttc cactagccgg gggatccggg aatttaaatc gcaaccgttt cgtgtcccgc
     1081 tgcaattcaa tgcgtttcac tttttcaagt tgtttgatgc gggattgcac ctggctggct
     1141 ttgttggcct ggtaccggaa ccggtcgacg aagttttgga cgcgtgaaat ttctttagat
     1201 tgtcgattgg cggccgcctc ttcctgggcg tcccgttctg cccggagctt ttggaagcgc
     1261 gtataattgc cgcggtattc ctgcagagta tgatttcgga cttcccagat atgggtgacc
     1321 atgttgtcta aaaaacccgt atcatggctg atgatcaaca gcgtgaaatt cgaagtgagt
     1381 aggaatgatt ccagccaacg ctgtgttggt ttatcgaggt ggttggtcgg ctcgtccagc
     1441 atgaggacgt cgggagctga cagcaataag tgggcaaggg ccacccgcat gcgatagcca
     1501 ccggagaggg tatcaatttt tcgttcccaa tccgactcct gaaatcctaa gccggcaaga
     1561 atccgcttgg cttcgtgatc ggggaattgg ttccgatgtg tagcatcgag aatggtgttt
     1621 cccggcaggg actccagttc ctgtggcaga tagccaaggc gaagaaaagg acgttgtcgg
     1681 atttttccgc tttcgaattc ctcctcagtg aggatcatgc gcatcagtga ggttttgcct
     1741 gacccattcg gaccaacaag tcccacacgg gtgttgggtt tgagatgtgc cgaggcctta
     1801 ataaataggg gttttgtggg gtggctcttg gaaatttgtt caatatgcaa catgcggaag
     1861 caccatccaa tatgtgagat gaaaaccgag tcaaaacccc tttgtcatgg tcaaaaagac
     1921 ggaaag
//
LOCUS       DAORAQ010000373         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8654_length_1918_cov_73.305958, whole genome shotgun sequence.
ACCESSION   DAORAQ010000373 DAORAQ010000000
VERSION     DAORAQ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8654_length_1918_cov_73.305958"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PKM72_15405"
     CDS             complement(<1..1553)
                     /locus_tag="PKM72_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNP62233.1"
                     /translation="MEKAQKVVLLGIDAGSRDLLKRWAQDGTLPTLQALFAKSLVGAT
                     TSTPGLFVGSTWPTWYTGVNPGKHGIHSLHQLQPGTYELHPTYAGESVKREPFWNHLS
                     RAGRRVAILDIPLTALSHNLNGIQLVEYGAHDAHSGFMTWPPSLAAEVEQRFGSPCSH
                     PIQGDCDAKRDAAGVAAFRDTLINGVARKTAVTKHFLQQDNWDLFAQVFTESHCIGHQ
                     YWHVHDPTHQWHDADLARIVGDPIKDVYKAIDTAIGEILKDIDEETTVIVFVSHGMGQ
                     TNIPYGFMEKFLLQLKVAVPPRIPANNVTGSLLQRRSLAFLYRMWQKIPSSVQKFLAP
                     IIYPLSHKLSADSGPLVDRAASKCFSIHDTPSHAGIRVNLVGREPEGKVQPGPDYQQF
                     CMELEKDLLGIINVKTGKPVIQRIIRTADHYSGENLAHLPDLLVEWYEEDPIPAVFSE
                     KFGKISTNFWHPRTGHHRAGGMFLAFGPSIQPGTVDRTVSLMDYAPTIAQLLNVSLPD
                     VDGQPIEELL"
BASE COUNT          487 a          472 c          480 g          479 t
ORIGIN      
        1 agaagttcct caatcggctg gccatcaaca tcaggaaggg agacattgag caactgtgca
       61 atcgtggggg cataatccat aagggacacc gttcgatcga cagttcccgg ttgaatagat
      121 ggtccgaagg ccaggaacat gccccccgct cggtgatgcc ctgtacgggg atgccagaaa
      181 tttgtcgaga ttttcccgaa tttttctgaa aaaaccgcag gaattggatc ttcttcatac
      241 cattccacca ggaggtcggg cagatgagca aggttctcac cggaataatg atcggctgtg
      301 cgaataattc gctggatcac cggctttcct gtcttgacgt tgatgatccc taacaaatcc
      361 ttttcgagct ccatacaaaa ttgttgatag tccggccccg gctggacctt gccttcgggt
      421 tcccggccaa ccagattcac tctgatcccg gcatgggaag gcgtgtcatg aatggaaaag
      481 cacttgctcg ccgctcgatc cacaagtggg ccagagtctg cggacagttt atgagaaaga
      541 ggatatatta tgggcgcaag aaatttctgc acgctcgatg ggatcttctg ccacattcgg
      601 tacaggaagg cgagtgatct tcgttgaaga agagagcctg tgacattgtt cgccggtatt
      661 cttggcggca cagccacctt taactgaagc aggaatttct ccatgaatcc atatggaata
      721 ttcgtctgcc ccataccatg gctgacaaaa acgatcaccg tggtttcttc gtcaatatcc
      781 ttgagaattt ctccaatggc cgtgtcgatg gctttataaa catccttgat cggatcccca
      841 acgatcctcg caagatccgc atcatgccat tgatgggtgg gatcgtgtac atgccagtac
      901 tgatgaccaa tacagtgact ttcggtgaaa acctgggcaa acaaatccca attatcttgt
      961 tgcaaaaaat gtttcgtgac cgcagtcttt ctggcgaccc cattgataag agtatcccga
     1021 aaggccgcga ctccagccgc atcccgtttt gcatcacagt ctccctggat aggatgagag
     1081 cacggtgaac caaagcgttg ttccacctca gcagccagcg agggtggcca cgtcataaat
     1141 ccggaatgtg catcgtgggc gccatattca accaattgaa tcccgttgag attgtgcgac
     1201 agggcagtca gaggaatatc aaggatggct acccttcgcc ctgcccggct tagatggttc
     1261 caaaaaggtt cacgtttaac gctttctcct gcatacgtgg gatgcagctc atacgttccc
     1321 ggctgaagct ggtgtaggga atgaattccg tgcttacccg ggttgacgcc ggtataccat
     1381 gtgggccacg tcgaaccgac aaaaagccct ggggtgctcg tggtggctcc caccaaactt
     1441 tttgcgaata gggcttgcag ggtcggcagg gttccatctt gcgcccatcg ttttaataaa
     1501 tctctcgatc ccgcatcaat acccagcaat acaacttttt gggctttttc caacggatta
     1561 ctcctcagaa attcaaccgc ggaatttcta caccatgcaa tgagaagagg tttgaagttg
     1621 tcacaaaatt ttcaatacga tggttcagtt cccgctcgtc tgaccgagac ggcctaaaga
     1681 cattaattga aaaaatgcga aatatcaaac cattcctgga taatagtaga agcctgcgtt
     1741 tggaatggac cgtaaagtat tggggtaccg ggaagacgct agtagtttct cgccagggca
     1801 gcttaaacgg atttctctat tagccaatgg aaggtaggta gggttgcgat gcgattggca
     1861 tacgttattg attcctgctc atgggtcacg acctttacga tcaatgaaat ggtagaga
//
LOCUS       DAORAQ010000374         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_8845_length_1890_cov_64.524796, whole genome shotgun sequence.
ACCESSION   DAORAQ010000374 DAORAQ010000000
VERSION     DAORAQ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8845_length_1890_cov_64.524796"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1019)
                     /locus_tag="PKM72_15410"
     CDS             complement(78..1019)
                     /locus_tag="PKM72_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62234.1"
                     /translation="MQYFEGIPCPPDVNIPTEDGDAWAWYPQYKWIYNKLMIAESQNL
                     PCAPHGLDPSFFPVFSKPIYNMRGMGAGSRVLRSLKEYKHLQRPGHLWMPLLEGDHVS
                     SDVAVVKGEPTWWRHATGKPLEGGMFDYWTVLAAPNPTIETYCGDWLRRHLAGYTGML
                     NFETIGGKIIEVHLRFADQWPDLYGNGWVEALIRLYAEEKWHFTESHRQEGYSVVLFG
                     AHGVHYQHPPKDVIDQLRHTPQVSSIQITFHEEKPPKDHSMPPGGFRLAIVNCWDLET
                     GFEVRKKLALSFWSTHEFQPNRFPAPKTIRDSAAESH"
     gene            1539..>1890
                     /locus_tag="PKM72_15415"
     CDS             1539..>1890
                     /locus_tag="PKM72_15415"
                     /inference="COORDINATES: protein motif:HMM:NF023621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HNP62235.1"
                     /translation="MSFFSKILDKLGFSGSEAQAAPSPQSPAPKSPAPQAAPATPAPS
                     PGTTVVDVKGKLEGLAASNSQKLNWKTSIVDLLKLLDLDSSFAARKELATELGCPADK
                     MGDSAQMNMWLHKTV"
BASE COUNT          448 a          472 c          491 g          479 t
ORIGIN      
        1 gttttcgatt tccgtaatac ggtcgagtat ggtgtggtcg atgaacatca aaaatctctg
       61 agtttttcga caatcggtta atgactttca gcagccgagt cgcggatagt ttttggagcg
      121 gggaatcgat tgggttgaaa ctcatgggtg gaccaaaaac tcagcgccag cttttttctg
      181 acttcgaatc ccgtttcaag atcccagcag ttgacgatgg ccagacggaa tccgcctggc
      241 ggcatggagt ggtccttcgg aggtttttct tcatggaacg tgatttgaat gctggagacc
      301 tgtggcgtgt ggcgcaactg gtcaatcacg tccttgggtg gatgctgata atggacaccg
      361 tgggcaccaa acaagacgac gctgtatcct tcctgtcgat gggattcggt gaagtgccat
      421 ttttcttccg catataggcg gatgagagcc tcaacccaac cattgccgta caaatcaggc
      481 cattggtctg caaaacgcaa atgcacctcg atgattttgc cgccgatcgt ctcaaagttg
      541 agcataccgg tgtatccggc aagatgccgg cgcagccagt caccgcaata ggtttcgatg
      601 gtgggattgg gcgccgctaa caccgtccaa taatcgaaca ttcctccctc cagcggtttg
      661 ccggtggcat gccgccacca tgttggttcg cccttaacta cggcgacgtc gcttgagaca
      721 tgatcgccct ccagaagcgg catccacagg tgtccggggc gttgtaagtg tttgtactct
      781 tttaatgagc gtaggacgcg acttcccgct cccataccgc gcatgttgta aatcggtttt
      841 gaaaacacgg gaaagaagga tggatccagc ccgtgcggag cacacggcag gttttggctt
      901 tccgcaatca tcagtttgtt atagatccac ttgtactgtg ggtaccatgc ccaggcatcg
      961 ccgtcttcag tcgggatgtt gacatcaggc gggcagggaa tgccttcgaa atactgcatt
     1021 ctccatggat caccttcaca aatcggcata tgcgccattt cgtttaatat cattcatttc
     1081 tgatgcggaa ggcataccat acccagtgag atgaggtcat gcaacacgca ttttcaagtc
     1141 cattggccat aggttcatca ccttggttga ggccttgggg attctcactc ctcatattcc
     1201 agcatcctac ccacgaattc tttggaacca ttacgtacag gtgagtgggg cgttcccctg
     1261 cgcggtctgt tacaagtgga cgaaatgcaa tgaatcttcc tgtttcccag cacatccgga
     1321 aagatgtccc gaatagacga atccatgatc gagtagacaa aagtatccat ttcaatcaag
     1381 atgggaatgt ctcatgatga tcgctgtagt cttgaacaat gttgaggggc gcctgtggag
     1441 cacgtgagga ggaaaatgaa gaaccggatt atacgagtgg aaaaacagtt ttctgttcct
     1501 tccgacatgg ttattatctc aaaaagaaag gacctgccat gagttttttt agcaaaatcc
     1561 ttgataagct cggtttcagc ggttccgaag cacaagctgc tccttcgcca caatcaccag
     1621 ccccgaaatc gcccgctcct caggctgccc ctgctacgcc ggctccttca cctggcacca
     1681 cagtggtgga tgtcaaaggg aagctagagg gcctcgcggc ctcgaactct caaaagctta
     1741 attggaaaac ttcgattgtg gatctgttga agctcctgga tcttgatagc agcttcgcag
     1801 ctcgtaagga actcgcgact gagctcgggt gtccggctga caaaatgggg gattccgccc
     1861 agatgaatat gtggttgcat aaaaccgtgt
//
LOCUS       DAORAQ010000375         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9142_length_1852_cov_72.558709, whole genome shotgun sequence.
ACCESSION   DAORAQ010000375 DAORAQ010000000
VERSION     DAORAQ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9142_length_1852_cov_72.558709"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..372)
                     /locus_tag="PKM72_15420"
     CDS             complement(37..372)
                     /locus_tag="PKM72_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNP62236.1"
                     /translation="MASLLKGSDCVDAHREMRDDNQLVCTVARSSEVMGYVVVDSTVC
                     GISREGLRMSPNVTEAEVRGLALTMTLKGGFLRQLQGGAKAGVRFNPEAQLAECRQHL
                     EAFGRLLRF"
     gene            complement(393..527)
                     /locus_tag="PKM72_15425"
     CDS             complement(393..527)
                     /locus_tag="PKM72_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62237.1"
                     /translation="MKFGIYPISRWQEMPKRFLREQGFSTYQDIPHAISPATSWLFEY
                     "
     gene            complement(652..1398)
                     /locus_tag="PKM72_15430"
     CDS             complement(652..1398)
                     /locus_tag="PKM72_15430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNP62238.1"
                     /translation="MFSEYRAIFKKRGHLYHQAMTLYPGARAEEFFHIVRMADMKDGD
                     IVCDIPSGGGYLSNFVDRTTTLCHIETSKVFAALCRANGVPQVLLSTLEDIPIETGAV
                     DKIISLAALHHVDEKNRFFSEAYRMLRKGGTLTIADVQAGSAVSEFLDGFVNEHNTMG
                     HKGTYISAETQDEIEGWGFTVRESSLIPFHWEFDSPQAMGRFSQLLFGIDKADSAQIL
                     EGIRKHLGYDFDSTACRMNWELLFLNAKKP"
     gene            1630..>1852
                     /locus_tag="PKM72_15435"
     CDS             1630..>1852
                     /locus_tag="PKM72_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62239.1"
                     /translation="MPHGRLVCVGYVIVSDGGRPRQVTVVSENQRRVSKEVEDLLIRS
                     LPPDQVVIGSLVFIHELDSPLPLEAKKSVI"
BASE COUNT          461 a          507 c          430 g          454 t
ORIGIN      
        1 tacccatatc agagccaaga ataaagatgc ggttaattag aagcggagca atcgcccaaa
       61 cgcttccaag tgctgccggc attctgcgag ctgggcctca gggttgaaac gcacgcctgc
      121 cttcgcgcca ccttgtagtt gtcttaagaa gccgcccttg agggtcatcg taagggctag
      181 accacgcact tctgcttcgg tcacattggg tgacatgcga agcccctctc gcgagatgcc
      241 acatacggtg gaatcaacga cgacgtaccc cataacttcg gagcttcgag cgaccgtgca
      301 gacaagttgg ttgtcatccc tcatctctcg atgcgcatcg acgcagtcac ttcccttgag
      361 taaagatgcc atcgagaatc ccattcaaaa cttcaatact cgaacaacca tgaagtggcc
      421 ggcgagatgg catgcgggat atcttgatag gtgctgaatc cttgctcacg caaaaaccgt
      481 ttgggcattt cttgccagcg gcttatagga taaattccaa acttcatgtt caaatgaatc
      541 atcaaagatc agactttggt ttaaaaggat cttttcattc gttcgcagca tccactaacc
      601 gagctattgc ctgtttacca ggtaccccat atcgctggac acgattgctg tctagggttt
      661 ttttgcattg aggaataaca gctcccaatt catgcgacac gcagtgctgt cgaaatcata
      721 gcccaaatgc tttcggatac cctcgaggat ctgggcagag tctgctttat cgatcccgaa
      781 caacagttga gaaaatcgtc ccatggcctg gggagaatca aactcccagt gaaaagggat
      841 caacgaagac tccctcacag taaatcccca tccttcgatc tcatcttgag tttcggcgga
      901 gatatatgtc cctttgtgtc ccattgtatt gtgttcgttg acaaaaccgt ccaaaaattc
      961 ggaaacggcc gaccccgctt gcacgtcagc aatggtaagc gtccctccct ttcttagcat
     1021 tcgataggcc tctgagaaga accggttctt ttcatcgaca tggtgcaatg ccgcgaggct
     1081 gataatttta tccaccgccc cggtctcgat aggaatatcc tccagagttg aaagcaacac
     1141 ctggggcacg ccattcgctc tgcagagagc agcaaacacc tttgaggttt cgatatggca
     1201 aagagtcgta gtccgatcca cgaagttact taagtaccct ccgccggaag gaatatcaca
     1261 aacgatatct ccgtctttca tgtcggccat acgtacgata tgaaagaatt cttctgcacg
     1321 agcgcccgga tacagggtca ttgcttgatg atatagatgg cctcgttttt tgaaaattgc
     1381 gcgatattcc gaaaacatca atcacctcgt attctttttg cccgtctcgc aattccaatg
     1441 cgattgtgac gatgtcagct gattcacgcc taacatcgga agaatccgtt caaaattgca
     1501 gaattccatc ccaccaacat gaggtatccc gcctttctcc tgtccagaaa gcgcacccac
     1561 ctcctgtgaa ccatatccgg ttggcaccgc cacaaagagt cgttgcgggc ctgccatcca
     1621 caacgagcga tgcctcatgg acgcttggtc tgcgtgggct acgtcattgt gtctgatggc
     1681 ggacggcccc gccaggtgac cgtggtttct gaaaatcaac ggcgcgtcag caaggaagtc
     1741 gaggacctgc tcattcggtc gctcccaccg gaccaggtag tcatagggag tctggtattc
     1801 atccatgagc tggactcccc acttcctctt gaagcgaaga agtccgtcat tg
//
LOCUS       DAORAQ010000376         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9178_length_1847_cov_81.387277, whole genome shotgun sequence.
ACCESSION   DAORAQ010000376 DAORAQ010000000
VERSION     DAORAQ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9178_length_1847_cov_81.387277"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..463
                     /locus_tag="PKM72_15440"
     CDS             146..463
                     /locus_tag="PKM72_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNP62240.1"
                     /translation="MSDTLNSMSMKLYHTEWCPECQVVREKLDELGLPYEHEVVPDVR
                     PFRKKLYEVSGQNYVPVLVHGETVLTETSDILAYLDDYDDHGAPSGEATKPPSSHLDE
                     RNH"
     gene            467..1591
                     /locus_tag="PKM72_15445"
     CDS             467..1591
                     /locus_tag="PKM72_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNP62241.1"
                     /translation="MDDWKKVLAASITKPKDLAKHLGVDPKEVEAVVGPYPMRITPTV
                     LATIKSKGDAIWKQVVPEAIELDDIDAPDDPLEEDTDSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDRAVAYLRERTEVRDVILSGGDPLLLPDRLIER
                     ILKGLRSIPHLELIRFGTRVPGTLPQRITPELCDIVKRFHPIYMNLHFNHPDELTPEV
                     KEACGRLADAGVPLGAQTVLLKGVNDDPEIMKRLMHQLLLARVKPYYLYQADLTKGTN
                     HFRTTVETGLKIIQSLQGHTSGMAVPHFVIDAPGGGGKIPLLPNDYLLNLDENGAVLK
                     NYENKTYHYPQPTSGNGRELPMVGAPASQDMCGAGAMDDY"
     gene            1591..>1847
                     /locus_tag="PKM72_15450"
     CDS             1591..>1847
                     /locus_tag="PKM72_15450"
                     /inference="COORDINATES: protein motif:HMM:NF018287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HNP62242.1"
                     /translation="MLDSKKTGILSFQHLRALVRQRVIRADVPITPKQIQPASLDLRL
                     GTKAYRLLSSFLPEPSDQQSQFTIEDLYRSDMVMYDMDLTNG"
BASE COUNT          459 a          454 c          487 g          447 t
ORIGIN      
        1 ataaaaaaat tattcatgga atgcgggatg ccgtcatggc tccctgttcc gtcatggaaa
       61 gtcacctgag ctcataaggc ccaggcctcc tggttctttc ggtcagagtg taatattcct
      121 ctcatgtgga agacccctag ttgacatgtc tgatactctt aacagcatga gcatgaaact
      181 gtatcacacg gagtggtgtc cggaatgcca agttgtccgc gagaaattgg acgaattggg
      241 actgccctac gaacatgaag ttgtaccgga tgttcgcccg ttccggaaaa aactatatga
      301 agtctcagga caaaattatg tcccggtgtt ggttcatgga gaaacggtgt tgacggagac
      361 atccgatatc ttggcatatc tggacgacta cgatgaccat ggcgctccct ccggggaagc
      421 caccaagcca ccctccagcc accttgatga aaggaatcat taagctatgg atgattggaa
      481 aaaggtcctc gccgcaagta tcaccaaacc caaggattta gccaaacatc ttggggtcga
      541 tcccaaggag gtggaagccg tcgtcggtcc ctatccgatg cgaattaccc cgactgtgct
      601 ggcaacaatt aaaagtaaag gggatgcgat ctggaaacag gtcgtccccg aggccattga
      661 actggatgat attgatgccc cggacgatcc gttggaggaa gatacggaca gcccggttcc
      721 tcacttggtc caccgatatc ctgatcgcgt cttgctgatg gtgaccaacc aatgtccaat
      781 ctattgccgg ttctgcacga gaaaacggct ggttggaaaa ccggggtttc tcaagaaggg
      841 tgagttggat cgagcggtgg cgtacctcag agagcgtact gaagttcggg acgtcatcct
      901 gtctgggggt gacccgttgt tgttgcctga tcgcctcatt gagagaattc tgaaaggcct
      961 tcgatccatc ccgcatttag aattgattcg gtttgggacc cgtgtgccgg ggactctccc
     1021 tcaacggatt actccagaat tgtgtgatat cgtgaaacgc ttccatccca tctatatgaa
     1081 tttgcatttc aaccatccgg atgagttgac gccggaagtc aaggaggctt gcggtcgatt
     1141 ggccgatgcc ggggtgccct tgggagccca aaccgtcttg ctgaaagggg tgaatgatga
     1201 tcctgaaatc atgaaacgcc tgatgcatca actgttgttg gccagagtga aaccctatta
     1261 cctctatcaa gctgacctca ccaaggggac gaatcatttt cggaccacgg tggaaacggg
     1321 gttgaaaatt attcaatccc ttcaggggca taccagtggc atggctgttc cccactttgt
     1381 cattgatgcg cctggaggtg gcggaaagat tccactgttg cccaacgatt atttattgaa
     1441 tttagatgag aacggggcag tcttaaaaaa ttacgaaaat aaaacctatc attatcctca
     1501 gccgacttcg ggaaacgggc gtgaattgcc aatggtaggc gccccagcgt cccaggatat
     1561 gtgcggcgcc ggcgccatgg atgattattg atgctggatt cgaagaaaac aggcattctg
     1621 tcttttcaac atcttcgtgc gctggttcgt cagcgcgtga tccgagccga tgtgcccatc
     1681 acccccaagc aaattcaacc ggccagtctc gatttacggc tgggaactaa ggcctaccgt
     1741 ctcctgagca gttttctgcc agagccatcc gatcagcaat cacagtttac gattgaggac
     1801 ctctaccggt ccgatatggt gatgtacgat atggatctga ccaatgg
//
LOCUS       DAORAQ010000377         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9280_length_1833_cov_70.604049, whole genome shotgun sequence.
ACCESSION   DAORAQ010000377 DAORAQ010000000
VERSION     DAORAQ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9280_length_1833_cov_70.604049"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..985)
                     /gene="msrA"
                     /locus_tag="PKM72_15455"
     CDS             complement(359..985)
                     /gene="msrA"
                     /locus_tag="PKM72_15455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNP62243.1"
                     /translation="MKRVLSHLFSLGLVALLIGGMTVIHHGNLAAEESQYAKATFAGG
                     CFWCMEEVYEKVDGVVSVVSGYTGGQLSNPTYEQVSAGGTGHTESVEVTYDPTKVTYQ
                     HLLEVFWHNVDPTTPNAQFCDHGNQYRTAIFYHDEGQKMLIDESKKALESSKSFPQPI
                     VTEIVPVSVFFSAEAYHQDFYKKNPVRYKFYKWNCGRSQRLEELWGKS"
     gene            1550..>1833
                     /locus_tag="PKM72_15460"
     CDS             1550..>1833
                     /locus_tag="PKM72_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62244.1"
                     /translation="MVRTVLWLVVGSLLLPFVSGSAFSADSIPLGDRLGLRTGSHSNV
                     CGSPTSSSLGRSSHFFSQSTPCNESTGEYSLTRFNFTQKREPFNLQGARD"
BASE COUNT          507 a          435 c          369 g          522 t
ORIGIN      
        1 ttaggcagga acaatgcaat cgaatcttca tacagtgatg gaatatatca gaccacatca
       61 gaagatgtcg gacaggaatc gtttctgtgg caatgacgag ggtggaagaa ccccgatcaa
      121 acagcccaac gagaaaatga cattttcatc ccatctacct cctccccatt atcccataga
      181 agaaaataac ttaagagtgg ttttaggaca aaacgacgag ggggggagga gtctcatgga
      241 gttttggcag tactcttgcg aagagtcccc tgaatcaaaa ggcagcctag ccccctttca
      301 atcccaagac tataaaacaa aatccagaaa ggggtggggc gggatgacta caaaaagatt
      361 atgactttcc ccataactct tccaaacgct gggaacgacc acaattccat ttataaaatt
      421 tgtagcggac cggattcttt ttataaaaat cctgatgata ggcttcagca gagaagaaga
      481 ccgaaactgg aacaatctcg gtcacaattg gttggggaaa ggactttgaa ctttctaacg
      541 cctttttgga ttcatcaatt aacatttttt gcccttcgtc atggtagaat attgcggtcc
      601 gatattgatt accatgatca cagaactggg cattcggggt tgtaggatca acattatgcc
      661 aaaacacctc caataaatgt tgatacgtga ccttggtagg atcgtaggtc acttcaacgg
      721 attcagtatg acccgttcct ccggccgaaa cttgttcata tgttggattg gacaactgcc
      781 ctcccgtata ccctgaaaca accgatacca cgccatcaac tttttcatac acttcttcca
      841 tgcaccagaa acaacctcct gcaaatgtag ccttggcata ttgactttcc tcggcggcaa
      901 gatttccatg atggatgact gtcattcccc ctatcaacag tgccaccaat cctagactga
      961 ataaatgaga tagtacccgc ttcattagta cctccagatt ttcacccctt cgttcaagaa
     1021 ttatcggcct cttagttaag caaaatatct ttattcccat gaagatagga ttactcctta
     1081 gtctcaatac actgaattgc gttacatggc ctgattccta gtgaatggcc acagtcctca
     1141 cttgtatcat tttgggtatc ctttgtgact tttggatact gatattcgat tatttcaaca
     1201 cttgctcatc agaaataggc accggagtta ctgattttca gatgggtcgg ctgcctgcca
     1261 acaccttatt tagttcttat ttcaccacaa ttcctctgtg gttataaacg cacagggaac
     1321 ctgtgcctgg aatcacgcag attcactcaa gatttttcca cataggaaaa ttcctccagc
     1381 tcctgagatt gttaccatcg ggaatattag taatgagatc aactcttttc tttttcttct
     1441 gaaaaaaatc aacaattatg gaggaaattt ttgcccgttt tgcactacgg catccgggtt
     1501 gcttaccgat aaggaatagg agaaaaaatc ataaataagg gggcaggtga tggtgcggac
     1561 tgttctctgg ttggttgttg gaagtttgct tctcccattc gtgagtggct cagccttttc
     1621 tgcggattcg atcccgctgg gtgatcgcct aggtctgcga acgggctcac attccaatgt
     1681 atgtggttcc ccgacctctt cctcattagg acgttcttcc cattttttct ctcaaagcac
     1741 tccctgtaat gaatccacgg gtgaatattc tctcactcgt ttcaacttta cccaaaaacg
     1801 tgaacccttc aaccttcagg gcgctcgaga tat
//
LOCUS       DAORAQ010000378         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9306_length_1830_cov_72.096338, whole genome shotgun sequence.
ACCESSION   DAORAQ010000378 DAORAQ010000000
VERSION     DAORAQ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9306_length_1830_cov_72.096338"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1193)
                     /locus_tag="PKM72_15465"
     CDS             complement(393..1193)
                     /locus_tag="PKM72_15465"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNP62245.1"
                     /translation="MSRLLMCPPDYFGIEYEINPWMRVSNQSNGDRARTQWKTLTQVL
                     ENEVGVKLEFMEPVPGLPDLVFTANAGVLHEGKAVPSQFRHPERQGEERHFIDWFTRK
                     GYEVINLDPDINFEGAGDLLGFPDFWIGGYRQRSDIRAYVELSEIFQKEIMPVELVDQ
                     RFYHLDTCFCPLSGGELLYFPPCFDTYAQTVLASRIEPDKRFAVPSKEGERFACNAVC
                     IDRHVVLPTGCPETMAWLHKRGYITHPVELDEFLKAGGSAKCLTLALD"
     gene            complement(1209..>1830)
                     /locus_tag="PKM72_15470"
     CDS             complement(1209..>1830)
                     /locus_tag="PKM72_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNP62246.1"
                     /translation="KVLFAGGPAIIHAGGREALAWIIEAGYIHVLFCGNALAAHDMEA
                     SLYGTSLGYNLGIGRSMPHGHEHHLRTINRVRALGSIRKAIESGLIKDGIMAACIRQG
                     VQMVLAGTIRDDGPLPDVITDSIKAQEAMRAAIPGVGLALLVASTLHAVATGNLLPAS
                     SPTVCVDINPAVPTKLSDRGSFQAVGLVMDSSSFLWELARELGWKG"
BASE COUNT          416 a          485 c          488 g          441 t
ORIGIN      
        1 tttccgctcc cacctgctga tgacaaaagt ggcactcccc tttttggatc tgggctttcc
       61 accatggggt tttccgtaat tttttcccgc tttcgcgctc aatccgtata tgccttcgga
      121 tcgcgtcatc gtcaccgagt gagtagaatt gttctggttg ggtcataagg gaagggagcc
      181 tctgttcata ttaattgatt cggactggtg atcacggaaa tcaggtgccg ggtcaacctg
      241 ttttggaaga ttgtagcgct tgggtcggcg ctttttaaag acaaggaggt ttttaggcct
      301 gggcacagat ccttcgcaag gcctcaggat cacaatgtag gagaggtcaa ctgcagtttg
      361 caggattgat gtgggaattg aagctggtgc gattaatcca gcgcgagagt taggcatttg
      421 gcggagccac cggccttgag gaattcatcc agttccacag gatgggtgat atagccccgt
      481 ttgtgtaacc atgccatcgt ttccggacat ccggtcggca gcacgacatg gcggtcgata
      541 cagaccgcat tgcaggcaaa tcgctcgcct tctttggatg ggactgcaaa tcgtttgtct
      601 ggttctatac gagaagccag cacagtttgg gcgtacgtat cgaaacaggg gggaaagtag
      661 aggagctctc ctccgctgag gggacaaaag caggtatcca gatgataaaa ccgttgatcc
      721 accagttcca caggcatgat ttctttctgg aaaatttccg acaactcgac gtaagcgcgg
      781 atatcactcc gttgccgata gccgccgatc cagaaatccg gaaatcccaa taagtccccg
      841 gccccttcaa agtttatgtc ggggtcaagg ttgatgactt catagccctt cctggtgaac
      901 caatcaatga aatgccgttc ctcaccctgt cgttccgggt ggcggaattg gctgggaaca
      961 gccttaccct catggaggac acccgcattg gcggtaaaca ccaaatcagg tagtcccggt
     1021 accggttcca taaattcgag tttgactcca acctcgttct ccagtacctg ggtcagcgtt
     1081 ttccattggg ttctggcccg atctccattg gattgattcg acactctcat ccatggatta
     1141 atttcatatt cgataccaaa ataatccggg gggcacatca ataaacggct catggtatgg
     1201 gtaccccgct atcctttcca gccgagttca cgggccaatt cccataaaaa agaagaagaa
     1261 tccatcacca gcccgacggc ttggaagctt ccacggtcac tcagttttgt gggaacggca
     1321 ggattgatat cgacacagac ggttggtgat gacgccggta gcaaatttcc agtcgccacg
     1381 gcatgcaggg tggaggccac caacagggcc aaccccacgc cgggaatggc agcgcgcatg
     1441 gcctcttggg ctttgatgga atcggtgatg acgtcaggca aagggccatc gtcgcgaatc
     1501 gtcccggcca gcaccatctg gaccccctgt cgtatgcagg ccgccatgat tccatctttg
     1561 attaatccgc tttcgatggc ttttcggatg cttcccaggg cccgcacacg attgatggtg
     1621 cgaaggtggt gctcatgtcc gtgaggcatg gaacggccaa tacccagatt gtatcccaag
     1681 gacgttccat ataggctggc ttccatgtcg tgagcggcca gggcgtttcc gcaaaacagg
     1741 acatggatgt atcccgcttc gatgatccat gccagggctt cccgtcctcc cgcatggatg
     1801 atggccggac cacccgcaaa caacacctta
//
LOCUS       DAORAQ010000379         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9423_length_1817_cov_49.674801, whole genome shotgun sequence.
ACCESSION   DAORAQ010000379 DAORAQ010000000
VERSION     DAORAQ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_1817_cov_49.674801"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1425
                     /locus_tag="PKM72_15475"
     CDS             313..1425
                     /locus_tag="PKM72_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNP62247.1"
                     /translation="MTTLFEPLHIGSLTLPNRIIMAPLTRMRSQQPGNVPHELNAEYY
                     AQRASAGLIISEATQISQQGQGYPGTPGIHSPDQVQGWKLVTDAVHKAGGRIFLQLWH
                     VGRISHTSHQPDGALPVAPSALAAENSGTYTVDWQETPIEVPRALETDEIPGIVGDFK
                     AGAENAKAAGFDGVEVHGANGYLLDQFLQDGSNSRTDHYGGSIQNRARLLLEVVDSVI
                     EVWGKDRVGVRLSPFNAFNGMKDSDPIKLFTYVLGALDSRGVAFVHLIEPRSSFAGMQ
                     DDSLEDQPHTASIFRPALKTILISAGGHTPVSAYEYVENNLADAVAFGRHFISNPDLP
                     ERIRQSSPLTPYDRATFYGGDTKGYTDYLPLNEQAA"
     gene            1459..>1817
                     /locus_tag="PKM72_15480"
     CDS             1459..>1817
                     /locus_tag="PKM72_15480"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNP62248.1"
                     /translation="MKPPISDIAFTPSVKAVQERMGSRKPYARMEEGDGWSSTITQEL
                     ADFVRKQDSLYLGTANKDGRPYIQHRGGPKGFLKVLDDHTLAFVDFAGNRQYISLGNL
                     AENHQAFLFLMDYATQTR"
BASE COUNT          473 a          465 c          444 g          435 t
ORIGIN      
        1 ggttacggac gaagaggttg ccaaactccg caaggccggg tttacggacg gggagatcac
       61 agaaatcctg gccaatatcg ggctgacact gtttaccaat tattttaacc acgtggcggg
      121 aacggaagtg gattttcctg cggtttccga actggaagtc agggtctaaa accgtccagg
      181 tctaaattca gaatcaccat tactcaatca ttcatgtcat gaaagaaaaa aaccaagacc
      241 acacaaactg cccttaacgt tcaagacaga ctgaagatca agaaatattt atttcattaa
      301 aggaaacgga tcatgacaac actatttgag cccttacaca tcggttccct aaccttaccc
      361 aatcgcatca tcatggcacc gttaacacgg atgcgatcac agcagccagg aaatgttcct
      421 cacgagctca atgccgaata ttatgctcag cgtgcgtcag ccggtttgat tatttctgaa
      481 gccacgcaaa tctcccaaca gggacaaggc taccctggta cccctggcat acattcccct
      541 gatcaggtcc aaggctggaa actcgtgacc gatgccgtgc ataaagctgg cggccggatc
      601 tttctccaac tctggcatgt ggggcgtatt tctcacacat ctcatcaacc cgatggtgcg
      661 ttgccggttg cgccctctgc tcttgctgcg gaaaacagcg ggacgtatac ggtcgattgg
      721 caggaaacgc cgattgaggt tccgcgcgca ttagaaacag atgaaattcc gggaattgtt
      781 ggcgatttta aagcaggggc tgaaaacgcc aaagcggcgg gttttgatgg cgtggaagtc
      841 catggagcca atgggtattt actcgatcag ttccttcagg atggttccaa ctcacgcact
      901 gaccactatg gtggatccat tcagaatcgt gcccgcctgt tgttggaggt tgtcgattcc
      961 gtcatcgaag tttgggggaa agatcgtgtc ggcgtgcgtt tatctccctt taacgcgttt
     1021 aacggcatga aggatagcga tcccatcaag ctgttcacgt atgtccttgg tgcgctggat
     1081 tcacgaggcg tggcctttgt ccatttaatt gagccacgtt catcattcgc gggcatgcaa
     1141 gacgatagcc ttgaagacca accacacacc gcctcaatat ttcgtcctgc acttaagacc
     1201 atcctgattt ccgccggagg ccatacccct gtttcagcct atgaatatgt agaaaataat
     1261 ttggctgatg ctgtcgcgtt tggaaggcac tttatttcca acccagacct cccggagcgt
     1321 atccgccagt cctcacctct caccccctat gatcgtgcca ctttttatgg cggagatacg
     1381 aagggatata ccgactatct tcctctgaat gaacaggcgg cctaacaacc acttgagtga
     1441 ggaaaaggag actaagccat gaaaccacca atcagtgata tcgcgttcac cccatccgta
     1501 aaggccgtcc aagagaggat gggatcacgt aaaccttatg ctcgaatgga agaaggagac
     1561 ggatggtcga gcaccatcac gcaagagctt gcggattttg tgaggaagca ggattcattg
     1621 tatctgggca cggccaacaa ggacggaagg ccgtacatcc aacatcgagg aggccccaaa
     1681 ggttttctca aggtactgga cgaccacact ctggcttttg ttgattttgc aggcaatcgc
     1741 caatatatct cattagggaa tttggccgaa aatcatcagg cttttttgtt cctgatggat
     1801 tacgcgaccc aaacccg
//
LOCUS       DAORAQ010000380         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9519_length_1806_cov_66.608795, whole genome shotgun sequence.
ACCESSION   DAORAQ010000380 DAORAQ010000000
VERSION     DAORAQ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9519_length_1806_cov_66.608795"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKM72_15485"
     CDS             <1..440
                     /locus_tag="PKM72_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNP62249.1"
                     /translation="LEFYDRDIRGVSISFKLPSVMRLLNMVKLRSNHHAVKFSRINIF
                     TRDRYTCQYCSTRFRTEELTFDHVVPIAKGGRKTWENIVTACWRCNNRKSGRTPKEAG
                     MKLLKEPVKPKWTPRLTLMIGIRHAPESWRDYLYWNVELDADS"
     gene            complement(715..867)
                     /locus_tag="PKM72_15490"
     CDS             complement(715..867)
                     /locus_tag="PKM72_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62250.1"
                     /translation="MYRCEKCQGTMLLDREVDMESGMSLLVFWCINCGLRKQAERAPI
                     PLIEVS"
     gene            1293..>1806
                     /locus_tag="PKM72_15495"
     CDS             1293..>1806
                     /locus_tag="PKM72_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62251.1"
                     /translation="MDLLPNIQPPVPAEPEWVSWSDEQLLNLRLSDLKIRISGTELGQ
                     CVRQLYRELKDQGLVFRPHIWLSDDWYSPDGTPGLAVPFYMGHPRLAKLELNQMLEVE
                     GGTPEWCMRILRHETGHAIENAYRLRRRRSRQQMFGHTSVPYPDCYLPKPYSKSFVLH
                     LDMWYAQSHPD"
BASE COUNT          480 a          395 c          458 g          473 t
ORIGIN      
        1 tccttgaatt ttatgatcgg gatattcgag gggtgtccat ttcctttaaa ttgccatccg
       61 tgatgcgtct cctcaatatg gtcaaactcc ggtcgaatca tcatgcggtg aaattttctc
      121 gcatcaatat ttttacacgg gatcggtata cctgccaata ttgctccaca cggtttcgaa
      181 cggaggaatt aacctttgac catgtggtgc ctattgccaa aggcgggagg aaaacctggg
      241 agaatatagt gacggcatgc tggcgatgta ataaccggaa aagcggtcgt actcctaaag
      301 aggcagggat gaagttgctc aaggagcccg tcaagcctaa atggactcca cgcttgacac
      361 tcatgatcgg catccgccat gccccggaaa gttggcggga ttatttatat tggaatgtgg
      421 aattggatgc ggattcctag attctctaat tctcctggtt cctgatggtt gttagcagga
      481 acattgttct atggtttctg ctgagggacg accaaaacgg aacttcatgc cctcacaaac
      541 agcgaattct acacattagg ttggacccga gctattgcgc gatgagaagg gtcctcactg
      601 atctgtccac ttcctcacaa ttctttgaaa atttataccc tagacccgaa caggcaggac
      661 gttcaggttt tatctgccaa tccgggtcaa ggaattgagg aagcaagcac taagctagga
      721 cacttcgatg aggggaatcg gtgctcgctc ggcttgtttc cgtaggccac aattaataca
      781 ccagaagacc agaagagaca ttcccgattc catgtcgact tcccgatcaa gtaacatcgt
      841 tccctggcat ttttcacagc gatacataga tgaatctcct gtggttctac cggtcaggct
      901 taaggagtca tcagaatcaa tatggaagag acttaccggc gtttggccaa aaatgtatga
      961 agaccaaaaa aatgaatgaa gccttgggca ctgattcttc agacgcgctg actcggagtg
     1021 tgaataaaaa actgcatggt actgaatgaa ggatttctca aaaagagggg caatctacca
     1081 taggcaaaaa ggtaaaagct atggaattca agggggcaac tgagaattgg tgaagcatgg
     1141 tttcactttt gacttatagc ggaaatggag tgaagattta cacatcatct gccggttgat
     1201 gcgtcatggt ttgtcaaatg ttctagacgg attccagcga aagcgtatga gatgatgatc
     1261 aaaaaccgac actggcagag gaggttgtga gtttggatct attgcctaat attcagcccc
     1321 ctgtcccagc agagcctgaa tgggtttcct ggtctgatga acagctgttg aatctccggt
     1381 tgtccgattt aaagatccgt atttcaggaa ctgaattggg tcaatgtgta cgccagctat
     1441 accgcgaatt gaaagatcaa gggttggttt ttcgtcccca catatggttg tctgacgatt
     1501 ggtattcacc cgatggcacg ccgggtcttg ccgtcccgtt ttatatgggg catccgcggt
     1561 tagcgaaact ggaactcaat caaatgttgg aagtggaggg cggaaccccg gaatggtgta
     1621 tgcggattct tcgacacgaa acgggtcatg cgattgagaa tgcctaccgc ttacggcggc
     1681 ggcgcagccg acaacaaatg ttcggacata cttcggttcc gtatcccgat tgttatttgc
     1741 caaagcccta cagtaaaagt tttgtgttgc atcttgatat gtggtatgcc cagagtcatc
     1801 ccgacg
//
LOCUS       DAORAQ010000381         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9607_length_1793_cov_54.699655, whole genome shotgun sequence.
ACCESSION   DAORAQ010000381 DAORAQ010000000
VERSION     DAORAQ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9607_length_1793_cov_54.699655"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKM72_15500"
     CDS             <1..798
                     /locus_tag="PKM72_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNP62252.1"
                     /translation="PLVLFAIYGLYFYVSNQEIVPLTGRSQLVDMTHEQEMALGLQSY
                     QEILSQSQVIPEGQVVDLVRTIGRRLANAAADVDPGFDWEFNVIESQQANAFALPGGK
                     TAVYTGLIPVAENQSGLAVVMGHEIAHAIARHGAERMAHQKLVQMGTIAATVAVGDMD
                     YDTQRMVMGALGVGAQYGVLLPFSRDHESEADYMGLLFVARACFDPTEAPKLWERMGE
                     MSQGKQPAEFISTHPSHGTRIKQFQEWMPEALALRQQHCGNSPPSSS"
     gene            complement(859..1383)
                     /locus_tag="PKM72_15505"
     CDS             complement(859..1383)
                     /locus_tag="PKM72_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HNP62253.1"
                     /translation="MSPINLDTLKALLKEYVTQRQYSIDLILVGGLALQAYGVHERVT
                     VDVDGELAGDLEPLLEFLRARQVPADLGENISGWSVVAMPPGYRNRTSVWYEAGGLRI
                     RLLHPVDFVIAKLRRGTEQDLDDAQEVVTRFHLSPADIQVAADSAIQASPKDTALFTF
                     RKILQVFDQRISGS"
     gene            complement(1364..1639)
                     /locus_tag="PKM72_15510"
     CDS             complement(1364..1639)
                     /locus_tag="PKM72_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62254.1"
                     /translation="MNTVEAILQVPRARRLLWLEILFNDQLDLKPWQDLPEVQDAYQK
                     ACQWFTTYRSLIQSVLPRVSLPLDNGKIDQRDYRTFAEALQFVTHQP"
BASE COUNT          455 a          490 c          448 g          400 t
ORIGIN      
        1 ccgctcgtcc tattcgcgat atacggactg tatttttacg tctcgaacca ggaaatcgtg
       61 ccactgaccg gccgttccca attggtggac atgacccatg agcaggaaat ggccttaggg
      121 ctgcaatcgt atcaagaaat cctcagccag tcccaagtca ttccggaagg gcaggtcgtg
      181 gatctggtcc gaaccatcgg acgccgtctg gcaaatgcgg cagccgatgt ggatccagga
      241 ttcgactggg aatttaatgt cattgaatct caacaggcca atgcctttgc cttacccggg
      301 ggaaaaaccg ccgtgtatac cggacttatt ccagttgcgg aaaaccaaag cgggttagcc
      361 gtcgtcatgg gccatgaaat cgcccatgcc atcgcccgtc atggggccga gcgcatggcg
      421 catcaaaaat tagtgcagat gggcaccatc gccgccacag tggcggtcgg cgatatggat
      481 tacgacacac agagaatggt catgggggca ctgggtgtcg gcgcacaata tggcgtgctc
      541 ctcccctttt cccgcgacca cgagtcggaa gccgattaca tgggcctgct ttttgtggcc
      601 cgtgcctgtt tcgaccccac agaagcacca aaactctggg agcgcatggg agaaatgagt
      661 caagggaaac aaccggccga attcatttcc acccatccca gccacgggac ccgcatcaaa
      721 caatttcaag aatggatgcc cgaagccctc gcccttcgcc aacaacattg tggcaactcg
      781 ccaccttcct catcataacc taacaacccc attcctgctt ttcttcgttg tctcctgacc
      841 ctgccttagg ttgccgagtc aactcccaga gattcgctgg tcaaaaactt ggagaatttt
      901 ccgaaaggta aacaaggcag tgtctttagg agaggcctgt atggctgaat ctgcggcaac
      961 ttgtatatcg gcaggagaaa gatgaaatcg agtgacaact tcttgagcat catccaaatc
     1021 ttgctccgtt ccacgacgga gtttggcaat cacaaaatca accggatgaa ggagtctgat
     1081 tcgaaggcct ccggcttcat accacactga agtccggttg cgatatcctg gaggcatcgc
     1141 caccactgac catccagata tgttttcccc aagatccgcc ggcacctgac gggctcggag
     1201 gaattccaaa agcggctcaa ggtctccagc caactcccca tcaacatcga ccgtgacgcg
     1261 ttcatgaacc ccataagcct gaagagcgag tccaccaacg aggatcagat caatagaata
     1321 ttgcctctga gttacgtatt ccttaaggag tgctttgagc gtatcaaggt tgatgggtga
     1381 caaattgtaa agcctccgca aacgtccgat agtctcgttg gtcaattttt ccgttgtcga
     1441 gagggagcga cacccgaggc aacacggatt gaatcaaact tctatacgtg gtgaaccact
     1501 ggcaagcttt ctgataggcg tcctgaacct ccggaagatc ctgccagggc ttgaggtcaa
     1561 gttgatcgtt aaaaagaatt tcaagccaaa gtaaccgtct ggcccggggg acttgaagaa
     1621 tcgcctcaac agtattcatc cccgtatagg ccggtaaaat tttgagggca ggagatgagg
     1681 agggaattga attgttcggc atcccggcaa gtgtaagcca ataaggcctt tccatcaagc
     1741 tcaaactctg cctcccgttg cagaatgact acgggtttcc tccttctttc aat
//
LOCUS       DAORAQ010000382         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_9825_length_1767_cov_66.306075, whole genome shotgun sequence.
ACCESSION   DAORAQ010000382 DAORAQ010000000
VERSION     DAORAQ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9825_length_1767_cov_66.306075"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1467
                     /locus_tag="PKM72_15515"
     CDS             25..1467
                     /locus_tag="PKM72_15515"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HNP62255.1"
                     /translation="MPWHIHQTLEEEKKFRIPPGFQLPPDMGEPIPPRVFTSTYFDSE
                     HHRLGQLGLTLRKRVEQSHGVWQLKLPSGGNRLELEIASGSRSIPGEFQDLLRSFFRK
                     QEAVQIGKLRTRRKGVRIRKDGQVIAELVQDSVALIRDKRIVHSFQEVEVELQGGTDT
                     QLNPVRKILLHAGAEEKPLQPKIFQALQLPYPLTVKFSDSSAPPTDHIRESLQSQYLQ
                     MLQNDPGTRLGRDSEALHQMRVATRRMRAIIRAVSAFLAPQWTEPVRQELAWVGSLLG
                     EVRDWDVLLESFHQNFHDFSPSEQRSFHTILKNFDDQRSVARAKLLEGLGSDRYLNLL
                     THFENSLIHLPFQPNPFTLTELARKAFQKIQDRANTSDSLFRKSELHHTRRLLKRARY
                     AVELAEPLLGKRAKRFIQQAKVVQDLLGFHQDAVVAEQRLLAFKNHSRGTGVAYVTGL
                     MVERLRNQQSQVYQQIPKQWQKLEKRGKKL"
     gene            1596..>1767
                     /locus_tag="PKM72_15520"
     CDS             1596..>1767
                     /locus_tag="PKM72_15520"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62256.1"
                     /translation="MTHQTKPKASNTPEVIDNLDDPKLYLNRELSWLEFNKRVLEEAE
                     DPSHPLLERVKFL"
BASE COUNT          509 a          435 c          407 g          416 t
ORIGIN      
        1 ataaagattg ccgggtaatt ttatatgcca tggcacattc accaaaccct tgaagaggaa
       61 aagaagtttc gtattccacc cggctttcaa ttgcctcccg acatggggga accgatcccg
      121 ccacgcgtat tcacatccac ctactttgac agcgagcacc atcgactcgg ccaactgggt
      181 ctgacactcc gaaaacgagt ggaacagtcc catggggtct ggcaattaaa gctaccgagc
      241 ggcggtaatc ggctggagtt ggaaattgcg tcaggcagtc gcagtattcc aggggaattt
      301 caggacttat taaggagttt tttcagaaaa caggaagctg ttcaaatcgg aaaactcaga
      361 acaaggagaa aaggggtacg cattcggaaa gacggccagg tcatcgctga acttgtccag
      421 gactccgtcg ccctcatcag agacaaaagg attgtgcatt cattccaaga agtggaggtt
      481 gaattacagg gaggaacaga cactcaatta aatcctgtcc gcaagattct gcttcatgcc
      541 ggggcagaag agaaaccatt acaacccaag atttttcaag cacttcaact tccctatccg
      601 cttaccgtta agtttagtga ttcttcagca ccaccgaccg accatattcg ggaaagcctt
      661 cagtcccagt atcttcaaat gttgcaaaat gatccaggta cccgattggg tcgggatagt
      721 gaagcgctcc atcaaatgcg ggttgccacg cgccgcatga gagccatcat tcgagccgtg
      781 agtgctttcc tggcgccgca atggacggaa ccggtccgcc aggaactggc ctgggttggt
      841 tcgttgttgg gcgaggtcag agattgggat gttcttttag aatcgtttca tcaaaatttc
      901 catgacttct ctccatccga acagcgttcc tttcacacga ttctcaagaa ttttgacgat
      961 cagaggtctg ttgcccgcgc caaactactt gaaggtttag gtagcgaccg ttatctcaat
     1021 ctcctgactc attttgaaaa ttcactgatt catttaccat ttcaaccaaa tccattcacg
     1081 ttaacagagt tggcgagaaa agcttttcag aaaattcagg atagggcgaa tacgtcagat
     1141 agcctattcc ggaaatctga actgcatcac actcgaagac tcctgaagcg agcccgatat
     1201 gcggttgaac tcgctgagcc cttgctcggg aaacgtgcca aacggttcat tcaacaagcg
     1261 aaggtcgtcc aggatttact gggattccac caagatgcgg tggttgctga gcaacggctc
     1321 ctcgccttca aaaatcactc acgaggaacc ggagtagcct atgtcacggg actaatggtc
     1381 gaacgcttac gaaatcagca atctcaggtg tatcagcaaa tccccaagca atggcaaaaa
     1441 cttgaaaaac gaggaaaaaa actgtaaagc cttacgccca atcttcttca aactgtttac
     1501 gtctccatat acccgtttaa tgataatggg acggaataac cttaatcagg tacaatgcct
     1561 cttaactcat aaaaggcata ataggagatc tgatcatgac tcatcagaca aaaccgaagg
     1621 catccaatac accggaagtt atcgacaatc tggatgaccc caaattgtat ctcaaccggg
     1681 aattgagttg gctggagttc aataaacggg tcctggaaga ggcagaagat ccgagccacc
     1741 ccctcctgga acgagtaaaa tttcttt
//
LOCUS       DAORAQ010000383         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_10185_length_1729_cov_64.523297, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000383 DAORAQ010000000
VERSION     DAORAQ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10185_length_1729_cov_64.523297"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKM72_15525"
     CDS             complement(<1..850)
                     /locus_tag="PKM72_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNP62257.1"
                     /translation="MTPMLDIYTMILAGGRGERLLPLTQHRAKPAVPFGGKYRIIDVT
                     LSNCLNSGLRKISVLIQYKSHSLDRHIRRGWSIFNPELGEFIFSIPPQQRISAEWYRG
                     TADAVHQNLFLLEQERPKFLLVLAGDHLYKMNYADMLRFMQETEAEAVVGALEWPVEE
                     ARQFGVLGVDASNRVTRFEEKPANPFHLPDDPTHAFISMGIYLFRTDHLYEELRRDAE
                     MATAHDFGKNIIPNMIQNNKKVFAYNFEDKNKKQVKYWRDIGTLDAYYEANMDLVGVD
                     PHLNLYD"
     gene            complement(961..1461)
                     /locus_tag="PKM72_15530"
     CDS             complement(961..1461)
                     /locus_tag="PKM72_15530"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNP62258.1"
                     /translation="MSTIYDIPCQSIQGKDETLAAKKGKLLLIVNTASKCGFTPQFQG
                     LEDLYQKYYAQGLEILGFPCNQFGSQDPGENEEILTFCQTQYGVTFPMFAKVDVNGPD
                     AHPLFKHLKTEAPGVLGSEAIKWNFTKFLVDQTGAVKERFAPATKPSDLEPAIKALLN
                     TPAKPA"
BASE COUNT          373 a          383 c          494 g          479 t
ORIGIN      
        1 catcatagag gttgaggtgg ggatcaaccc cgacgagatc catgttggct tcgtagtagg
       61 cgtccagggt accaatgtcc cgccagtact tgacttgttt tttgttttta tcttcaaaat
      121 tataggcgaa aacttttttg ttgttctgaa tcatgttggg aatgatattt ttcccaaagt
      181 catgggccgt ggccatctcg gcatctcttc gcagttcttc atagagatgg tctgtgcgaa
      241 aaaggtaaat gcccatggaa ataaacgcat gtgttggatc atcgggtaag tgaaacgggt
      301 tggccggctt ttcttcaaac ctggtgacac ggttggatgc atcgaccccg aggaccccga
      361 actgtcgtgc ttcttcgact ggccattcaa gggcgcctac gacggcctcc gcttccgtct
      421 cctgcatgaa tctgagcatg tcagcgtagt tcattttgta aaggtgatcg ccggccagca
      481 ctaataaaaa cttcgggcgt tcctgttcca gcaggaacag gttttgatgc accgcgtcgg
      541 ctgtgccccg gtaccattca gcgctgatac gttgctgcgg tgggatggaa aaaatgaatt
      601 cgcccagctc cgggttgaaa atcgaccaac ctcttcggat gtggcggtct aatgagtggg
      661 atttatattg aatgagcacg gaaattttgc gaagtcctga attgaggcaa ttgctcaagg
      721 tcacgtcgat gatgcggtat ttacctccaa agggcacagc aggtttggcg cggtgttggg
      781 tgagtgggag gagccgttca ccgcggcccc cggcaaggat catggtatag atatcaagca
      841 tgggtgtcat gatatcaaat gacccggatg gaaggaatgg gttgcgttcc ggctgttggt
      901 tggcgcgcaa gctccgagtt gtcaatggaa gttggatgag ctggaaaagc ctcgcgtcac
      961 ttatgccggc tttgccggag tattcagaag tgccttgatg gccggctcga gatctgaggg
     1021 tttggttgcg ggcgcaaagc gttctttgac ggcaccggtt tggtccacga gaaatttggt
     1081 gaagttccac ttgatggctt ctgatccgag tactcccggt gcctcggttt tgagatgttt
     1141 gaagagggga tgggcatccg gcccattgac gtcaactttg gcaaacatgg gaaaggttac
     1201 tccatactgt gtttggcaga acgtgaggat ttcttcgttc tctcccggat cctgacttcc
     1261 gaactggttg caaggaaatc caagaatttc caggccctgg gcgtaatatt tctgatagag
     1321 atcttcgagc ccttggaatt gaggggtgaa cccgcatttg gaggccgtat tgacgataag
     1381 taggagcttg cccttttttg cggcgagagt ctcatccttt ccttgaatgc tttggcaggg
     1441 aatatcgtaa atggtgctca tgggttttgt tcctttaaca ttatggagtt cgagtgcatg
     1501 aagtgctgct gtctttcact ctactcagtt gattggcccg agatcgtatc atttggatag
     1561 aggtttggcc atgcgatggt atcgtgaagg gaaatttgac acaatatgga ttgtgctgta
     1621 acctgtggtc tgttcatgtc gattcggtcc gacgacggga acatcctata aattttcggg
     1681 ccccgcgatc gtatcgcgac cagatcctgc tcggagcgga cttcccacc
//
LOCUS       DAORAQ010000384         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_10605_length_1682_cov_69.193608, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000384 DAORAQ010000000
VERSION     DAORAQ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10605_length_1682_cov_69.193608"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            623..>1682
                     /locus_tag="PKM72_15535"
     CDS             623..>1682
                     /locus_tag="PKM72_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62259.1"
                     /translation="MKNWIKIFLINIGVVILIFVLAEIGLRAYSTLKSCLMEQCDGSL
                     FTRFNLFHENKMIGLSTLDPLLGYKPNPGFRGLIHHPPYWDNVYVSIDRHGFRQHSDD
                     NTMSDHSGTKVLTVGDSFTFGDQVNNSQTWPACLAQKTGHKVLNGGVGGYGVLQSVLR
                     AEQEVAKHGPFDTIIWSITVGADSRRARLMVRSNFPKPHIATVEGKTRMVPPMDFNPS
                     PDFMWLLGYSVILQRHLLPILQKRWNFEYDGRYEIPGRNAAEIEDIIDFTFQQYAKLT
                     GTKRKVILLQYGQGIFLDEGFERRNNERELIKNYGKKYAIEIIDSFDEIYVPERREEL
                     WFGYRDHGHHTPYGNRVVC"
BASE COUNT          469 a          325 c          405 g          483 t
ORIGIN      
        1 tttggttacc ccaacctcaa cctcttatcc taaaccctta ttcaagtacc gccgggatgg
       61 gtgacatttg ctgatcggtt ggtgaaccag ctctgtcaag ctgtcccttc tttgtcggag
      121 cccatgatgg gatttccaaa ttgaagggac cctcaacctc ctagccgagg atgttcggga
      181 atggcagtaa gacaggcagc aaaaatcata agaggaaatc ttaaaagcct aaggctggga
      241 gcatttcggg acgatagata aaaggagtct gaatgcgctg tttctgaaat gggtgcaatt
      301 attctccagt cggcaatatg tcatccagcg gattgaaact ctgagtgagt aattcgttac
      361 ttctcatata atctgagaaa aggtttcgtc tattgatggt ttagcccgga gttgaatgaa
      421 ggttttccca tgcaaatttg ctgtagatga ttattcggtc atgggttcaa cccccaaatt
      481 aatagaaatg ccagcaatat ctatgaatta tctactacct tgctcaacgt aaagaaagga
      541 atgagttttg ctttcattgt aatctcggtt gagtagctag gtccctcacc caagttataa
      601 gttgaattag gatagggcaa ttttgaaaaa ttggattaaa atttttctca ttaatattgg
      661 cgtggtgatc ctaatttttg ttctcgccga aatcgggtta agggcctatt ctaccctcaa
      721 atcttgtctg atggagcaat gtgacggatc cttattcacg aggttcaatc tcttccatga
      781 aaataagatg attggccttt ccaccctcga tccccttctg gggtataagc ccaatccggg
      841 ttttaggggg ctcattcatc atccaccgta ttgggataac gtgtatgtct ctattgaccg
      901 ccacggattt cgtcaacata gcgatgacaa cacgatgagt gatcattccg gaacaaaagt
      961 gttaaccgtg ggtgatagtt tcacttttgg cgaccaagtg aataactctc aaacctggcc
     1021 ggcttgtctc gcacaaaaaa caggtcataa ggttttaaat ggcggagtgg gtggttatgg
     1081 agttcttcaa tcggtcttga gagctgaaca ggaagtcgcc aagcatggac ctttcgacac
     1141 cattatctgg tcgattacag tgggagcaga ttctcgtcgc gctcgattaa tggttcggtc
     1201 gaatttccct aagccccata tcgccacggt ggaaggaaag acgaggatgg tgccgccaat
     1261 ggattttaat ccttctccgg atttcatgtg gttgcttgga tattcggtga tccttcagag
     1321 gcatttgttg ccgatacttc agaaaagatg gaattttgaa tatgacggac gatatgaaat
     1381 tccagggagg aatgctgcag aaatagagga tatcattgat tttacctttc aacaatatgc
     1441 caagctaacg gggacgaaaa gaaaggtaat cttactacag tatggtcagg gaatttttct
     1501 ggacgagggc tttgaaagaa gaaacaatga acgagagctg attaaaaatt atgggaaaaa
     1561 atatgccatt gaaataattg attcatttga tgagatttat gttcctgaaa ggagggagga
     1621 gctttggttt ggatataggg accatggtca tcacacccct tatgggaata gagtggtctg
     1681 cg
//
LOCUS       DAORAQ010000385         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_11039_length_1638_cov_68.601390, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000385 DAORAQ010000000
VERSION     DAORAQ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11039_length_1638_cov_68.601390"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKM72_15540"
     CDS             complement(<1..1005)
                     /locus_tag="PKM72_15540"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNP62260.1"
                     /translation="MEKKAPVVKKARIGKRAPVALLGYSLETMEAAKEHNIPFVAVVP
                     EGFDIALKEDGVDVVTWDFYVHNQNSDRLYEKLVERGVSHAVPLYEETVEWAGSLNTR
                     LLKDPRAFQRALLFRDKAMMKRNAQMNGIRVGVFEEIRSREEALAFFDDINEARLRLQ
                     EEAPEPVHLKPLSAAGSVGHVFVRSRQDIISLPDDAFPCLAESHLAGQEFSVEAFIHK
                     GKIEFMNITEYVHLGYSQIVPETPKLSKHRTKIRKAIEKLMKAFGIVNGMIHPEYFLD
                     PEGELHFGEVAARVPGGHIFELIQRAHGFNPYAALLLCSDPNTSKTELDRFFPKEKDH
                     K"
     gene            complement(1123..>1638)
                     /locus_tag="PKM72_15545"
     CDS             complement(1123..>1638)
                     /locus_tag="PKM72_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNP62261.1"
                     /translation="RRIIHRRLDIQPAKRHEMVIAANILRSSADWYRPFLHEKDMAQH
                     EVDESWGEREFARRQFYIGREGNAPVGIVSTQSVGDMFYIGYIYVYDHQTGKGFGPQL
                     LNHVRDVARREGKRGMVLIAHPKAFWATRSYRRFGFECIASTRDEVLEWGGGWLKPYH
                     EEGFELYQYLF"
BASE COUNT          415 a          454 c          375 g          394 t
ORIGIN      
        1 tttatggtct ttttcttttg ggaaaaaccg gtccaattct gttttggagg tattgggatc
       61 agagcataac aacagagccg catacggatt aaagccatgg gcgcgctgaa tgagttcaaa
      121 aatatggccg ccgggaacac gggccgctac ctccccaaaa tgcaactccc cctccggatc
      181 caaaaaatat tccggatgaa tcataccatt aacgatgcca aatgccttca taagtttttc
      241 aatcgcctta cgaatctttg tccggtgttt gctgagcttc ggcgtttccg gcacgatctg
      301 ggaatagccc agatgcacgt actcggtaat attcataaat tcgatttttc ccttatggat
      361 aaaggcttcg accgaaaatt cctgaccggc caaatggctc tcggccagac aaggaaaggc
      421 atcgtcggga agggagatga tgtcttgtcg acttcgaaca aacacatggc ccacactccc
      481 agccgcggag agaggtttca gatgaaccgg ttccggcgct tcctcttgca agcgaagtct
      541 agcctcattg atatcatcaa aaaacgcgag tgcttcttcc cgtgagcgaa tttcttcaaa
      601 aaccccgaca cgaatgccgt tcatttgcgc attgcgcttc atcatggcct tgtcgcggaa
      661 cagcagagcc cgttgaaagg cacgtggatc tttaagcaga cgggtgttga gggacccggc
      721 ccattctacc gtttcttcat acagcggaac agcatgactc accccccgct ccaccaactt
      781 ttcatagagt cggtcggaat tctgattgtg cacataaaag tcccaggtca ccacatccac
      841 cccatcctct tttaatgcga tatcaaaacc ctccggtacc accgcgacga aggggatgtt
      901 gtgttccttt gcggcttcca tcgtttccaa gctatagccg agcagagcca ctggggccct
      961 ctttcctatt cgggctttct tgaccactgg ggctttcttt tccatgtcaa atctccttaa
     1021 gtgggggaca tatcgaataa actcctccat gctgtcgctg ctagcagaca aatggagaac
     1081 ccatcagcaa tgataattcg ctcgtttggg aggaaggacg tgttagaaca gatattggta
     1141 cagctcaaaa ccctcttcat ggtagggctt caaccaaccg ccgccccact ctaagacttc
     1201 gtcacgagtg gacgcaatac attcaaaacc gaaacggcga taggagcgcg tggcccaaaa
     1261 ggccttggga tgggcaatta acaccatgcc acgttttccc tcgcgccggg ccacatcgcg
     1321 gacgtgattg agtaattgag gcccaaaccc tttgccggtc tggtgatcat agacatagat
     1381 gtacccaata taaaacatgt ctccgactga ctgggtagat acgatgccga ccggcgcatt
     1441 cccttctcgc ccgatataaa actgccgccg agcaaactcc cgttcccccc aggattcatc
     1501 cacctcatgc tgtgccatat ccttctcatg aagaaagggt cgataccagt cagcagaaga
     1561 tctgaggata ttggcagcaa taaccatctc atgacgtttg gccggctgaa tatccagccg
     1621 ccgatgaata attctcct
//
LOCUS       DAORAQ010000386         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_11105_length_1631_cov_60.980330, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000386 DAORAQ010000000
VERSION     DAORAQ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11105_length_1631_cov_60.980330"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKM72_15550"
     CDS             <1..690
                     /locus_tag="PKM72_15550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNP62262.1"
                     /translation="GPFACLTGDFPMIRVVIFDVDGTLMDTNYLHVEAWARAFSVVKH
                     PVPRAAIHRQIGKGSDLMLGALLTDEALHGKANELHSEFFEELKDHTYPLPGAKEILA
                     SLSSQDIAIWLATSAKSEELEHHVQALEAKDKLAGMVSSADVDSAKPAPDIFQLTLER
                     AGCTPQESIVVGDTIWDIQSAQGCGLQTIAVRTGGAFSREELHAAGAVAVYQDCAELL
                     ASGFPTSFETR"
     gene            complement(846..1100)
                     /locus_tag="PKM72_15555"
     CDS             complement(846..1100)
                     /locus_tag="PKM72_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNP62263.1"
                     /translation="MSIIGWIVFGLIVGVVGKILMPGKDPGGFFATVAIGIIGALFGG
                     MLGRMVGMYGQDDPVGFVMAVIGAILFLWLYRVITRRGDT"
     gene            complement(1113..>1631)
                     /locus_tag="PKM72_15560"
     CDS             complement(1113..>1631)
                     /locus_tag="PKM72_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNP62264.1"
                     /translation="IMERLDHVMVAVVQGTMLTALAQGFAAGLTYGALGVPFAVFLGA
                     LSALFSLLPFGGTAFVWGPVAVYLFWTAPVWKGIVMIGIGIGLVGLMDNILQPLLVGS
                     KADLPVLFLFFASLGGLAYFGFIGLFLGPILLGIALAVFQIYRENYQEQAGLLIKAGS
                     GHPQEPENPTIK"
BASE COUNT          418 a          428 c          437 g          348 t
ORIGIN      
        1 ggccctttcg cctgtctgac aggagatttt cctatgatac gagtggtgat tttcgacgtg
       61 gatggcacgc ttatggatac gaattattta cacgttgagg cctgggcccg tgctttttcg
      121 gtagtcaaac atccagtccc gcgtgccgcg attcatcgtc agattggtaa gggatcggac
      181 ctgatgctgg gtgcgcttct tacggatgag gccttacacg gaaaggccaa tgagttgcac
      241 agcgaatttt ttgaggagtt gaaagatcat acctatcctt tgcctggagc caaagaaatc
      301 cttgcatctc tctcatccca agacatcgcc atctggttgg cgacgagtgc gaagtctgaa
      361 gaattggaac atcatgttca agcgcttgag gccaaggata agctggccgg gatggtgtca
      421 tcagcggatg tggacagtgc gaaacccgca ccggatattt ttcaactcac tctggagcgg
      481 gccggatgta ccccgcagga gagcattgtg gtcggggaca cgatttggga tattcaatcc
      541 gctcaaggtt gtggacttca gacgattgcg gtcagaaccg gaggggcctt tagtcgggag
      601 gaactccatg cggctggagc ggtggctgta taccaggatt gtgcagaact gctggcatcc
      661 gggtttccca cttcatttga aacgagatag gaaggagggg gcattcagcg atgggtgata
      721 ttaactgccc cgcggaatcc agacgaaatc ccctcattgt ttattctgct catgtggtag
      781 ctggaaacag acgagtcagc gaggggaaat aactgtcccc gacgatccgg ggaaaattgc
      841 tgacgtcagg tgtcaccacg gcgggtaatt acccgataaa gccagagaaa caggatggct
      901 ccgattacgg ccatcacaaa ccccacggga tcatcctgtc catacatgcc gaccattcgg
      961 ccgagcatcc caccaaaaag cgccccaatg ataccgatag caacagtggc aaaaaaccct
     1021 ccagggtctt tcccgggcat taagattttt ccaactactc ccacaattaa tccaaagacg
     1081 atccaaccga tgatactcat acctcatccc tcctatttaa ttgttgggtt ctccggctct
     1141 tgtgggtgtc ccgatcctgc ttttattaaa agacctgcct gctcctgata attttcacgg
     1201 taaatttgga aaaccgccag ggcgatccca agcagaattg gacccagaaa taatccgatg
     1261 aatccgaagt aggcgaggcc gcctaaggaa gcgaagaaga gaaacaggac cggcaggtcc
     1321 gctttggagc cgaccagaag aggttgcaga atattatcca tgagtccgac caatccgatt
     1381 cctatgccga tcatgacgat ccctttccat accggcgcgg tccaaaaaag atagacggcg
     1441 accggacccc agacgaatgc cgtgcctccg aacgggagaa gagaaaagag tgcgctcaga
     1501 gctcccagaa aaacggcaaa cgggacgccc agcgccccat aggtgaggcc agccgcgaac
     1561 ccctgtgcca acgcggtgag catggtaccc tgaacgaccg ccaccatcac atggtccagg
     1621 cgttccatga t
//
LOCUS       DAORAQ010000387         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_11414_length_1602_cov_85.012282, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000387 DAORAQ010000000
VERSION     DAORAQ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11414_length_1602_cov_85.012282"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1602
                     /locus_tag="PKM72_15565"
     CDS             <1..>1602
                     /locus_tag="PKM72_15565"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNP62265.1"
                     /translation="ALEDFHFREKMFHFDHERIPERVVHARGFGAHGYFENYESLADV
                     TRADLFQRPRDKTPAFVRFSTVAGNKGSFDLARDVRGFAVKLYTKEGNWDLVGNNMPV
                     FFIQDAMKFPDLVHAAKAEPDRGFPQAQTAHDNFWDFISLTPESMHMIMWTMSDRAIP
                     RSFRFMEGFGVHTFRLINGQGKSTFVKFHWKPKLGMQSVVWNEAVKINGADPDFHRRD
                     LWSAIQQGDYPEWELGLQLFDDNFAEQFNFDVLDATKLIPEEILPIRRVGRLVLDRCV
                     DNFFAETEQVAFCTQNIVPGIDFSNDPLLQGRNFSYLDTQLKRLGSPNFTHIPINAPK
                     CPFSHFQQDGHMAMQNPKGRANYEPNSWPGEAGGPRESPEKGFTSYPADEEGPKVRVR
                     SETFADHYSQARQFYISQTEVEQTHIANALVFELSKVEHPEIRTRMVSHLLNIHQDLA
                     KQVAKGLRLKDMPKPADAAKPTREDLEQSPALSILLNSPNTFKGRKLGVLITDGVDIK
                     IFKSLKAAVKSEGAMMEIVAPGVGGV"
BASE COUNT          411 a          402 c          403 g          386 t
ORIGIN      
        1 cgcattggaa gactttcact ttcgggagaa gatgtttcat tttgaccatg aacggattcc
       61 agaacgggtt gtgcatgccc gagggttcgg agctcatggg tattttgaaa actacgaatc
      121 tctggcagat gtcaccagag ccgacctctt tcaacgacca cgtgataaaa ctccggcatt
      181 cgtccgcttt tctaccgtag ccgggaataa gggctccttc gatttggcaa gagacgtccg
      241 gggatttgcc gtaaagctgt ataccaaaga ggggaattgg gatcttgtgg gcaacaatat
      301 gcccgtgttc tttattcagg acgccatgaa atttccagac ctcgttcacg cagccaaagc
      361 ggaaccggat cgtggctttc cccaggcgca aaccgcgcat gataactttt gggactttat
      421 ctctctgacc cccgaaagta tgcatatgat catgtggacc atgtccgaca gggctattcc
      481 ccgatccttt cgtttcatgg aagggtttgg cgttcacaca ttccggctta tcaatggtca
      541 gggaaaatcg acgttcgtga aatttcactg gaaacccaaa ttggggatgc aatccgtggt
      601 gtggaacgaa gccgtaaaaa ttaatggagc cgacccggac ttccatcgcc gggatttatg
      661 gagtgccatt cagcagggtg attatccgga atgggaactg ggccttcagc tttttgatga
      721 taattttgct gaacaattca attttgatgt gctcgatgcg accaaactca ttccggaaga
      781 aattctacca attcgacgcg tgggccggct cgttctggat cgctgtgtgg acaatttctt
      841 tgctgagacc gaacaggtcg ccttttgtac gcaaaacatt gtgccgggca tcgatttttc
      901 aaatgacccc ttattacaag ggcggaactt ttcctacctc gacacgcagc tcaaacggct
      961 tggcagtccg aatttcactc acatccccat caacgctccc aaatgtccgt tcagtcattt
     1021 tcaacaggac ggccatatgg ccatgcagaa tccgaaaggc cgtgccaact atgaaccaaa
     1081 ttcctggccc ggggaagcgg gaggtcccag ggaatccccg gagaagggat ttacctcata
     1141 tcctgccgat gaggaagggc caaaagtacg ggttcggtcc gaaacttttg ccgatcatta
     1201 cagccaggcc cgacaatttt atatcagcca gaccgaagtg gaacaaaccc acatcgcgaa
     1261 tgccttagtc tttgaattaa gcaaagtgga acatcctgaa atccggactc gtatggtttc
     1321 tcatctgctg aatattcacc aggatttggc taaacaagta gccaagggtc tgcgcctgaa
     1381 ggacatgcct aagcctgcgg atgcggcaaa acctacacga gaggaccttg agcaatcacc
     1441 cgcgctcagc attcttctca atagtcccaa taccttcaaa gggcgaaagc ttggagtgct
     1501 catcaccgac ggagtcgata taaaaatatt taaatcactc aaagccgccg tcaagtcgga
     1561 aggggcgatg atggagattg tggcccccgg cgtgggggga gt
//
LOCUS       DAORAQ010000388         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_11780_length_1571_cov_101.914908, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000388 DAORAQ010000000
VERSION     DAORAQ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11780_length_1571_cov_101.914908"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..522
                     /gene="rplS"
                     /locus_tag="PKM72_15570"
     CDS             64..522
                     /gene="rplS"
                     /locus_tag="PKM72_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNP62266.1"
                     /translation="MNLLERLEQSLAQGTVPFFEIGDTVRVKVRVVEERQYGKKKEEA
                     KERIQVFEGLVIARKGRKVCENFTVRKISFGVGVERIFPLHSPNLAGIEVIRKGQVRR
                     AKLYYLRDKKGKAAKVSEREFSRTPKTVKNAAVAVDVPESGESAPTEVSE"
     gene            491..1201
                     /locus_tag="PKM72_15575"
     CDS             491..1201
                     /locus_tag="PKM72_15575"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNP62267.1"
                     /translation="MNQHPRKYRNNAGEGAHLSLPAASPSFMGAGPTDFFEQAARARG
                     FRRIAGLDEAGRGPLAGPVVAATVILPRCWSPVLLDDSKLLSERQRQVLYEAITTRAI
                     AWAIGLASEQEIDELNILGATRLAGFRALTQLAITPDYLLLDALHLPTVSLPQRPVIK
                     GDQLSRSIAAASILAKVSRDRMMISYHERFPQYQFHLHKGYPTQEHLRRLIQFGPCPG
                     HRRSFQPVQACLNARSLA"
     gene            1198..>1571
                     /locus_tag="PKM72_15580"
     CDS             1198..>1571
                     /locus_tag="PKM72_15580"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNP62268.1"
                     /translation="MTTPSQEFGRSAEELAEDCLRKKGYRILERNLRVGGGELDLIAQ
                     HHDTLVFIEVKARRTDQFGGAPYAIDARKKRQIIKLASYYLSQHGLSNQICRFDVILI
                     TGTRQGSPKLTHLEQAFEVSSS"
BASE COUNT          394 a          428 c          396 g          353 t
ORIGIN      
        1 attgaacaca actatacctc gacgtccagt cgtacgacga gaccttgaag gagggagact
       61 ctcatgaatt tattggagcg tttagagcaa tcgttagcac agggaaccgt gccgtttttt
      121 gaaataggtg acacggtcag agtcaaggtc cgcgttgttg aagaacgaca atacgggaaa
      181 aagaaggaag aggccaaaga acgcattcag gtgtttgaag ggctcgtgat cgctcgcaaa
      241 ggccgaaaag tatgtgagaa ttttaccgtt cgtaagattt cctttggagt cggagtcgag
      301 cgaattttcc cccttcactc ccctaatctg gccggcatcg aagtcataag aaaaggtcaa
      361 gtgcgacggg caaagctgta ttaccttcga gacaaaaaag gcaaagccgc caaagtttca
      421 gaacgggaat tttcgcgtac accaaaaacc gtgaaaaatg ctgcggtggc agtggatgta
      481 cctgagtccg gtgaatcagc acccacggaa gtatcggaat aacgcaggag aaggggctca
      541 cctttcattg ccagccgcct ctccctcatt catgggtgcg ggtccgacag acttttttga
      601 acaagcggcc agagcccgcg gattccggcg tattgcagga ctggacgagg ctggccgggg
      661 tcctctggcc gggccggtgg tcgccgcgac agtgatcctt ccccgttgct ggtcaccggt
      721 ccttctcgac gattccaagc tcctgtctga acgtcagcgt caggtattat atgaggccat
      781 taccaccagg gccatcgcct gggccattgg cctggcatca gagcaggaga ttgatgaact
      841 gaacatcctg ggcgccacac gattggccgg attccgggct cttacccaat tggccattac
      901 acctgattat ctcctccttg acgccctcca cctccctacc gtttctctcc ctcaacgacc
      961 ggtcatcaag ggcgaccagc tttccagatc gattgccgcc gcatccatcc tggccaaagt
     1021 atccagagat cgcatgatga tctcctacca cgaacgcttc cctcagtatc aatttcacct
     1081 ccacaaaggg tatcccacac aggaacatct cagacgatta attcaattcg gcccttgtcc
     1141 tggacatcga cgaagcttcc agcctgtcca agcctgcctg aatgcccgct cgctcgcatg
     1201 accacgcctt ctcaggagtt tggacgatcg gcggaggaat tagcagagga ttgtcttcga
     1261 aaaaaagggt accggattct tgagcgaaat ctgcgagtag gtgggggaga attggatctc
     1321 attgcacaac accatgacac actcgtattt attgaggtga aggcacgacg caccgatcaa
     1381 ttcgggggcg ccccctatgc cattgatgct cgcaaaaaac gccaaatcat taaattggcg
     1441 tcctattacc tttcccaaca tggcctcagc aaccaaattt gccggtttga tgttatcctg
     1501 attacaggaa ccagacaggg ttctccgaaa ttaactcacc tggaacaggc ttttgaggtt
     1561 tccagctccg a
//
LOCUS       DAORAQ010000389         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_12375_length_1525_cov_91.331293, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000389 DAORAQ010000000
VERSION     DAORAQ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12375_length_1525_cov_91.331293"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKM72_15585"
     CDS             <1..241
                     /locus_tag="PKM72_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62269.1"
                     /translation="DLLRPGGILVYSACSVEPEETTEIISDFCQKHHEFSQESVTPWV
                     PVAGQSLITDPGHLCTAFQTLNMDGFFACRLKKSE"
     gene            238..918
                     /gene="rpe"
                     /locus_tag="PKM72_15590"
     CDS             238..918
                     /gene="rpe"
                     /locus_tag="PKM72_15590"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNP62270.1"
                     /translation="MTMPITPLISPSILSADFARLAEAVQMVEAAGADWIHVDVMDGH
                     FVPNLTVGPPMVEALRKVTSLPLDVHLMMTNPDAFISEFVDAGADLLTVHVEACPHLH
                     RTVQSIKERQVKAGVSLNPATSVTAIEEILGDVDLILVMSVNPGFGGQQFISSTLEKI
                     RRIRTMMTNSHSSAHLEVDGGVNLTNVASVIHAGANVLVAGSAIFGSKNIPETIRQMR
                     TAAQTVIV"
     gene            927..>1525
                     /locus_tag="PKM72_15595"
     CDS             927..>1525
                     /locus_tag="PKM72_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62271.1"
                     /translation="MNSPNNTDSLHPLEIQVLRALGKDPKPLSDLQLATHTELAPSQV
                     SMAVGWLLTKQLVSLSTESTTTYVSLTTVGTQYHQPDSSPLEWIIQTVKNADPEGSPP
                     TVKDLQATGKFQPSELSRAIGILKKEGVLQMGAGGMLEITSKDSDTVRDLRSLINQVH
                     EGTRPLDSFTSDHQALLRQYAVKRGNTQEPFRIDDHTERT"
BASE COUNT          355 a          430 c          378 g          362 t
ORIGIN      
        1 tgacctcttg agaccagggg gaatcttggt ctatagtgcc tgctcagtcg aaccggagga
       61 gaccacggag attatctccg atttttgtca gaaacatcat gaattttctc aggaatccgt
      121 cacaccttgg gtgcccgttg cggggcaatc actcattact gaccctgggc atttgtgtac
      181 ggcctttcag acgttgaaca tggacggatt ttttgcgtgc cgattgaaaa aatctgaatg
      241 acaatgccaa tcaccccttt aatatcccct tctattcttt ctgctgactt tgcccggctc
      301 gcggaagccg tacagatggt agaagccgcc ggtgcggatt ggattcatgt ggatgtgatg
      361 gatggccatt tcgttcccaa tttgaccgtc ggtccgccca tggttgaagc gctccgcaag
      421 gtgacctcct tgcccttgga tgttcatctg atgatgacga atccggatgc cttcatttct
      481 gagttcgtcg atgccggcgc tgatctgttg accgtgcatg tcgaagcctg tccacattta
      541 caccgaaccg ttcaatctat taaggaacga caggtcaaag ccggagtgtc cttaaaccct
      601 gcgacctcgg tcaccgccat tgaggaaata cttggcgacg tggatttaat cctggtgatg
      661 tccgtgaatc ccgggtttgg tggccaacaa tttatctcct ccaccctgga gaagatccgg
      721 cgtatccgga cgatgatgac caattcccac agttcggccc atttagaagt ggatggggga
      781 gtcaacctga ctaacgtggc ttcggtgatt cacgcgggcg cgaatgtcct ggttgccggt
      841 tccgccattt ttggcagcaa gaatatcccc gaaaccattc gccagatgcg aacagccgct
      901 cagaccgtga ttgtctaatc gggcccatga attcccccaa taacaccgac agccttcatc
      961 ctctcgaaat tcaggtccta cgtgcactgg ggaaagaccc aaagccactc tcagaccttc
     1021 aacttgccac ccacaccgag cttgctccct cacaagtcag catggctgtc gggtggctac
     1081 tcacgaaaca attagtgagc ctgtcgaccg aatccacgac gacctatgtg tccctcacta
     1141 cggttgggac ccaataccac cagccggact cttctcctct tgagtggatc atccagacgg
     1201 tgaagaatgc tgatcccgaa ggctccccac ccaccgtaaa ggaccttcaa gccacgggga
     1261 aattccaacc ctccgagcta agccgggcaa tcgggattct taagaaggaa ggcgtccttc
     1321 agatgggtgc gggtggaatg ttggaaatta ccagcaagga cagtgacact gtgagggatt
     1381 tgcgaagcct catcaatcag gttcatgaag gcacacgccc cctggattcg tttacttccg
     1441 atcaccaggc attgcttcgc caatacgccg tcaaacgcgg caatacgcaa gaaccctttc
     1501 gcatcgatga tcataccgaa cggac
//
LOCUS       DAORAQ010000390         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_12488_length_1516_cov_56.529090, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000390 DAORAQ010000000
VERSION     DAORAQ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12488_length_1516_cov_56.529090"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..602)
                     /locus_tag="PKM72_15600"
     CDS             complement(6..602)
                     /locus_tag="PKM72_15600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNP62272.1"
                     /translation="MVPEATPEIAHDAEHHPLLDEVMKQLTDHRQAFHAFLTRRLGNP
                     AFAEDLLQQCFMKALAHFHSIKQMDRIIPWFYQILRHALIDYYRSKETDTKRFQAFLV
                     ETETLGTHQVPSLDELQPTICACLDRLVLTLKPEYAAIIRRIDLSGESLKSVAKDLQI
                     TTNNLTVRLHRARHALRKSLEDSCGICSKHGCLNCTCE"
     gene            complement(824..>1516)
                     /gene="deoC"
                     /locus_tag="PKM72_15605"
     CDS             complement(824..>1516)
                     /gene="deoC"
                     /locus_tag="PKM72_15605"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNP62273.1"
                     /translation="LIGACRMTRDEFTKIIDHTALKPETTKDQIRQLCEEAMTFGFGA
                     VCIAPLWVPFATRCLTGSPVRIATVIGFPHGNTLPDVKAYEAKLAIEAGAHELDMVIQ
                     LGLLRAGNHGAVLDDIQGVVNVARRAHSSKILVKVILETSLLNKEEQQIGCQLVEKAG
                     ADFVKTSTGFTGSGATIEGVGFLRGIVGQRLGVKAAAGIRDLISAVALVNAGASRLGS
                     SSSVSIMKMWPG"
BASE COUNT          355 a          377 c          429 g          355 t
ORIGIN      
        1 ggggcttatt cgcaggtgca attcaagcag ccatgcttac tgcaaatccc gcaggaatct
       61 tccaaggact ttcgcaaggc atgacgcgct cgatgcaaac ggaccgtgag gttattcgtg
      121 gtgatttgta aatccttggc gacggacttc agggattctc cggacaaatc aatccgacga
      181 atgatggcgg catattcggg cttgagggtc aatacgaggc gatccagaca ggcacaaatc
      241 gtcggttgga gttcatccag cgaagggacc tgatgggtgc ccagtgtctc ggtctcgaca
      301 agaaatgcct gaaaccgttt ggtgtccgtt tctttgcttc gataataatc aatcagcgca
      361 tgtcgaagga tttggtagaa ccaggggatg atccggtcca tctgtttgat ggaatggaaa
      421 tgggccaaag ctttcatgaa gcattgttgc aggaggtctt cggcaaaggc aggattgccg
      481 agtcggcggg tcaggaaggc gtgaaaggcc tggcgatgat cagtgagctg cttcatcacc
      541 tcatcgagaa ggggatggtg ttccgcatcg tgtgctattt caggtgtggc ttcgggtacc
      601 atttggcgct ccaggtcttc gatcaatcca aacggaattg gaccatatca tgcccggccg
      661 gcattcggga agaacggtac ttttaatgga agtgatgacc taaaaaaata gagtcccgct
      721 tacgaccggc gggaatgatg actcaaaagg gaaaaggaga aggaaaagga tagctgttca
      781 ttcaaaatcg tgagcgggtt tgaatgtgta atgaaggctg gctttaccct ggccacattt
      841 tcatgattga cacggacgac gaacttccca ggcgggaggc tccggcattg accaacgcca
      901 cagccgaaat caggtcgcgt atcccggcgg cagccttgac ccctaaccgt tgtccgacaa
      961 tgccccgcag aaaacccacg ccttcaatcg tggctccgct gccggtgaat cctgtcgatg
     1021 tttttacaaa gtcggccccg gccttctcta ccagttggca ccctatttgt tgttcttcct
     1081 tatttaagag tgacgtttcg agaataacct tgaccaggat ttttgaagag tgggcacgcc
     1141 gtgcgacgtt cacgacgccc tgtatatcgt ccaggacggc tccatgattg cctgctcgaa
     1201 gcaagccaag ctggatgacc atatccaatt catgcgcccc ggcttcaatc gccagtttgg
     1261 cctcataggc tttcacgtcc ggaagcgtgt tgccgtgagg aaagccaatg accgtggcaa
     1321 ttcggaccgg agatcccgta agacaccgcg tggcaaacgg aacccacagc ggggcgatgc
     1381 acaccgcccc gaagccgaac gtcatggctt cttcgcagag ctgtcggatc tggtctttgg
     1441 tggtttccgg ttttaaggcg gtatggtcaa taattttagt gaattcgtcg cgggtcatcc
     1501 tgcaggctcc gatgag
//
LOCUS       DAORAQ010000391         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_12578_length_1510_cov_62.394502, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000391 DAORAQ010000000
VERSION     DAORAQ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12578_length_1510_cov_62.394502"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1510)
                     /gene="uvrC"
                     /locus_tag="PKM72_15610"
     CDS             complement(<1..>1510)
                     /gene="uvrC"
                     /locus_tag="PKM72_15610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNP62274.1"
                     /translation="IYIGKAAVLSDRVRSYFQHGADLTPKSRVLYSQIADLETIITHS
                     EFEALLLESNLIKRHRPRFNVVLRDDKQYPYLRLPILDDFPRLSIVRRVKQDKALYFG
                     PYVPTGAMRDTLKVIRKVFPLATCKIDINGTAERACLEFEIKRCMAPCTGNQTKEDYH
                     QIVKQVRWFLEGRDTELLESLRSGMQEAAEREEYEEAARLRDQIGNIERMLAKQRVAQ
                     ITAREQDIMGLARLGGAADVQILFVRGGLLIGRRDFFWSDAHGVTDEELVRSTLEQFY
                     AKSVLPPKEILLPVGFDDLSLIQRWLSEKKEKTVRVLVPERGAKYDLLQLAQENAAVA
                     LKEHLRVQSESSDAVEELQTILGVPQIPARIECFDISNTMGTHSVASMVVWDQGRMKK
                     SDYRRFRIKTVEGANDFASMHEVVKRRYGGSLATKPGKTLPEPNLVVIDGGAGQLAAA
                     MEAMAEVGLSHVSICGLAKAKGEKDERIFLPGHKTPIILPLKSPATRLVQTIR"
BASE COUNT          343 a          428 c          373 g          366 t
ORIGIN      
        1 cggatggttt ggaccagccg cgtggcgggg gatttgaggg gcaggataat gggagtcttg
       61 tgacctggca aaaaaatccg ttcgtctttt tcacccttcg ctttggcgag gccgcagatg
      121 gagacatgcg aaagcccaac ttccgccatg gcttccatgg ccgcggccaa ttgtccggct
      181 ccaccatcaa tcacgaccag gttgggctcc ggcaacgttt ttcctggctt ggtagccaac
      241 gatccgccgt atcggcgttt caccacttca tgcatactgg cgaagtcgtt ggcgccttct
      301 accgttttaa tgcgaaatcg ccgatagtcg gacttcttca tcctgccttg atcccagacc
      361 accatggagg cgactgaatg ggttcccatg gtattggaaa tatcaaaaca ttcaatgcga
      421 gctggaatct gtggaacccc caggatcgtc tgaagctctt ccaccgcatc actggattcg
      481 gattgcaccc gaagatgttc cttgagagcc actgcggcat tttcctgtgc gagctggagc
      541 aaatcatatt tcgctccccg ttccggaacc aggacgcgaa cggttttctc ttttttctcg
      601 gacaaccagc gctggattag cgatagatca tcaaaaccca cgggaagtaa aatttctttg
      661 gggggaagga cggatttggc ataaaactgt tcaagcgtcg accgtaccag ttcttcatca
      721 gtgacgccgt gagcgtcgga ccagaagaaa tcccgacggc cgattaacaa gccgcctcgc
      781 acaaacagta tctgcacatc cgctgctcca ccaagtctag ccaaccccat gatatcctgc
      841 tcccgtgcag tgatttgcgc cacccgttgt ttggccaaca tccgttcaat gttccctatc
      901 tgatcacgta gacgcgcggc ttcttcatac tcttcccgct ctgccgcttc ctgcatgcct
      961 gaccgcaatg actcaagtaa ttcggtgtcc cgtccctcta aaaaccaacg tacctgcttc
     1021 acaatctgat gatagtcctc cttcgtctga tttcccgtgc agggggccat gcatcgcttg
     1081 atttcaaatt ccaggcaggc ccgctcagcg gtcccattta tgtcgatttt gcaggtggcc
     1141 agggggaaca ccttgcgaat cactttcaga gtgtcacgca tggcccctgt aggaacatac
     1201 gggccgaaat ataacgcctt gtcctgtttg acccggcgga cgatagacag cctcgggaaa
     1261 tcgtccagaa tcggcaaccg gagataggga tattgtttat catcccgaag gaccacattg
     1321 aaccggggac gatgacgctt gatcagattg ctttctaaca gaagggcttc gaattccgag
     1381 tgagtgatga tggtttccaa atccgcgatc tgcgaataca acacccggct tttaggtgtc
     1441 agatcggccc catgctgaaa atacgatcgc acccggtccg ataacacggc ggcttttcca
     1501 atatagataa
//
LOCUS       DAORAQ010000392         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_12589_length_1509_cov_59.268913, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000392 DAORAQ010000000
VERSION     DAORAQ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12589_length_1509_cov_59.268913"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PKM72_15615"
     CDS             <1..936
                     /locus_tag="PKM72_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNP62275.1"
                     /translation="ASWGIFREIIEYYKEQCRDNHFKPSISLSTNGVFGQSRLDYIAE
                     NCTHLTFSLDGVREVHDEHRVLPNGGSSFDRIIATMKGLEVRGRNYDIRSTVTAAGSA
                     TLAEFVAFVGENLQCKSLHFEPVFDATTVTNLKDQIAHLDAQMFVENFRTARQVAAGF
                     GIQLHYSAASLKHRETFCGASDASNFLVTSRGIVTSCNEVLQPTDPRSKLFQYGAWNQ
                     EDGEFVLDHEAIDRLGKLNVHDMPKCQGCIAKYNCAGDCYAKSASSTGDPASAGYTER
                     CHITRELLKDNLLLALIFKMAGVNAGGSVHHACPM"
     gene            complement(1081..>1509)
                     /locus_tag="PKM72_15620"
     CDS             complement(1081..>1509)
                     /locus_tag="PKM72_15620"
                     /inference="COORDINATES: protein motif:HMM:NF039960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62276.1"
                     /translation="ESAFRHDATPPWDWFLFIPLGPQSSAEGYAQAKDETWEEYEDEA
                     GGFFNSRSSMEDALDFIGWYNHKSSRDLGISKWDPKRLYLAYHEGRGGYARRSYKRKP
                     KVVRIANRVDWQARQYGAQLRQCEHRFKCRHWYQLWPFCS"
BASE COUNT          358 a          375 c          406 g          370 t
ORIGIN      
        1 gccagttggg gtatatttcg ggaaattatt gaatactaca aagaacagtg cagggacaac
       61 catttcaaac cctccatttc gttaagcacg aatggtgtgt ttggccaatc acggctggat
      121 tacattgcag aaaactgcac tcatctgacc ttctccttgg atggcgtgcg agaagtgcat
      181 gatgagcatc gggtcctgcc gaatggcgga agctccttcg atcgcattat tgccaccatg
      241 aaggggcttg aggtgcgtgg gaggaactac gatatccgat ccacggtaac cgcagcaggt
      301 agcgcgacgt tggcggaatt tgttgcgttt gtcggggaga acctacaatg caaaagtctt
      361 cacttcgaac cggtgttcga tgccacaaca gtgacaaacc tgaaggacca gattgcacat
      421 cttgatgcgc agatgttcgt tgagaacttc cgcaccgcaa gacaggtggc tgccggattt
      481 ggaatccaac tgcactattc agccgcttct ctgaagcacc gggagacgtt ttgtggagcc
      541 agtgatgcga gcaatttcct ggtcacctcg cgtggaattg tgacttcatg taacgaagtt
      601 cttcaaccta cggatccgcg gtccaagttg tttcaatatg gagcgtggaa tcaggaggat
      661 ggtgaattcg ttctggatca tgaagcgatt gatcgtttag ggaaactcaa tgttcatgat
      721 atgccgaagt gccaggggtg catcgcaaaa tataattgtg caggagactg ctatgccaag
      781 agtgcctcct cgaccggcga cccggcttcg gcgggataca cggaacggtg tcatatcacc
      841 cgggaattgc tcaaagataa tctgctcctt gcgctcattt ttaaaatggc tggtgttaac
      901 gcgggtgggt cagtccacca tgcttgtccc atgtaatttt taaaacgcgg acattatttt
      961 tcagaggtgt cattttgaga cagacaggcc taatcggaat ccggcaggat agagtaatcg
     1021 aatagagcaa attctgattg cccccgattc aatgccctgc cgtgatcaga ggctggagat
     1081 tcagctacaa aatggccata gctgatacca gtgccgacac ttgaaccgat gctcgcattg
     1141 acgcaactgc gcgccgtact gtctggcttg ccaatctaca cgattcgcga tgcgcacgac
     1201 ctttggcttt ctcttgtagc ttcgccgcgc atagcctccg cgaccttcat ggtaagccag
     1261 atacaggcgc ttgggatccc atttagagat gccgaggtct cggctgctct tgtggttgta
     1321 ccagccgatg aagtccaggg cgtcctccat ggatgagcgg ctattgaaga acccaccggc
     1381 ctcatcctca tattcctccc atgtctcatc tttagcttgg gcataacctt cggccgacga
     1441 ttggggtccg agtggaatga acaggaacca atcccatggg ggcgtcgcgt cgtggcgaaa
     1501 cgccgactc
//
LOCUS       DAORAQ010000393         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate AMR_MDS_0954
            NODE_12590_length_1509_cov_57.705640, whole genome shotgun
            sequence.
ACCESSION   DAORAQ010000393 DAORAQ010000000
VERSION     DAORAQ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852297
            Sequence Read Archive: SRR609198
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (wastewater metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:51:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,102
            Genes (coding)                    :: 3,096
            CDSs (with protein)               :: 3,096
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12590_length_1509_cov_57.705640"
                     /isolate="AMR_MDS_0954"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKM72_15625"
     CDS             <1..704
                     /locus_tag="PKM72_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62277.1"
                     /translation="EAMNTETTQVVVSRRKFSGKQVILVVGLAMLVMALGVVLWMNQY
                     VNAAMFQPTRLGESEQQVLNVKMAHLLHTADSPSSSVSQPSPSTVNLPIEPVPYSETD
                     ANRQIQLTEREVNALIATDSYMARHMAVDMADDLLSVQLVVPVKREMPLVGGRMLKVN
                     FGLALSYADGKPVVAMRGISLGGIPLPSAWWGDIKNTNLVEEFGGSGGFWDQFSKGVK
                     DMKIQDGQLYVMLKE"
     gene            complement(992..>1509)
                     /locus_tag="PKM72_15630"
     CDS             complement(992..>1509)
                     /locus_tag="PKM72_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNP62278.1"
                     /translation="MAQTKSLYVIVDTDKNIVLLKARGIPLRTFPLTRAEWIGDPLSH
                     STVLHLKTKDPSVSPLPISPPSASSPDSPPVDPITPLTVNNMPNRYELIFQESVTILV
                     QPSHLPSFWANMGHKIAGWGRRVTARIGTWSGSHQYLVLSLDPVEAQALYWAAIPPMT
                     CLVIPGNSTAP"
BASE COUNT          318 a          332 c          457 g          402 t
ORIGIN      
        1 gagaggcgat gaatactgag acaacccaag tcgtggtttc acggcgaaag tttagtggga
       61 aacaggttat ccttgtagtc gggcttgcga tgttggtcat ggcgttgggc gttgtattgt
      121 ggatgaacca gtacgtgaat gccgcgatgt ttcagccgac ccgattgggt gagtcggaac
      181 agcaggtcct gaatgtcaaa atggctcatt tgcttcatac ggccgattcc ccttcttcat
      241 ccgtgtccca accttctccc tccacggtaa acttgcccat tgagccggtg ccgtattctg
      301 aaacggatgc gaatcggcag attcagttaa ccgagcggga ggtgaatgcc ttgattgcca
      361 ccgattcgta tatggccaga catatggctg ttgatatggc ggatgatctt ctgagtgtcc
      421 aactggtggt cccggtaaaa cgcgagatgc cccttgtggg tggaaggatg ttgaaggtca
      481 attttggact ggcgctgagt tatgccgatg gaaagccggt ggtggcgatg cgcgggatta
      541 gtttaggcgg aattccgctt cccagtgcct ggtggggaga tatcaaaaat accaacctgg
      601 tggaagaatt tggcggctca ggcgggtttt gggatcaatt ttcgaagggc gtgaaggata
      661 tgaaaatcca ggatgggcaa ttgtatgtga tgttgaaaga gtagccgtcc ccatcaccag
      721 tgtaagcctg gtcatttgtc tggaacaggc ttgattttca agcatcccgg taacgtaccc
      781 tctccgactt tcgtgtcgat ccatgcgaag gggattttcc tacccggtgc tgttcctatt
      841 cagttgtttg aatcgaagtg cttcctgcct gcctggagtt cctgttctcc ggttgttatt
      901 ttaagtcccc gtctcccttg ttgttttgta atccctcgtt gatccttcag atggtcctac
      961 ccctctttct gttcactgcc ggagtctggt tttatggggc tgtggagttt cccggaatca
     1021 ccagacacgt cataggcggg atggccgccc aatacaaagc ctgggcttcc acaggatcaa
     1081 gtgagagcac caggtattgg tgggacccgc tccaggtgcc tattctggct gtgacccgtc
     1141 gcccccagcc ggctattttg tgtcccatgt ttgcccaaaa ggatgggagg tgagacggtt
     1201 gtaccaaaat agtcacggat tcttgaaaga tgagctcata gcggttgggc atattgttga
     1261 cggttaacgg agtgatggga tctaccggcg gagagtccgg agaagaagct gatggagggg
     1321 aaatgggcag gggggagacc gatgggtctt tcgtcttgag atgaagcacg gttgagtggg
     1381 agagagggtc tccgatccac tcggcgcgtg tgaggggaaa tgtccgcaag gggatgccac
     1441 gagctttgag caatacgatg ttcttgtcgg tatcgacgat gacatagaga gattttgttt
     1501 gtgccattt
//
